title: Changes in macrophage and lymphocyte functions in guinea-pigs after different amounts of vitamin E ingestion.
abstract: Vitamin E is the main biological lipid-soluble antioxidant and plays a crucial role in the maintenance of the immune system. In the present work, twenty-one guinea-pigs (3-weeks-old) were distributed into three groups, which during 5 weeks ingested different amounts of vitamin E (/kg diet): 15 mg (low vitamin E diet), 150 mg (medium vitamin E diet; control) or 1500 mg (high vitamin E diet). The function of lymphocytes and macrophages were then studied. In macrophages obtained from the peritoneum several steps of the phagocytic process (chemotaxis, ingestion and superoxide anion production) were assayed, as well as chemotaxis and proliferation of peritoneal and spleen lymphocytes. The results indicate that with respect to the medium vitamin E diet, low ingestion of vitamin E causes a decrease in chemotaxis and production of superoxide anion by macrophages and an increase in the phagocytic capacity. With the high vitamin E diet an increase in macrophage and lymphocyte chemotaxis, superoxide anion production and lymphoproliferative capacity, as well as a decrease in phagocytosis, were observed. Therefore, diet supplementation with higher than usual levels of vitamin E appears to be beneficial for the immune system.
authors.affiliation: Department of Animal Physiology, Faculty of Biological Science, Complutense University of Madrid, Spain. mondelaf@eucmax.sim.ucm.es
authors.forename: M
authors.initials: M
authors.lastname: De la Fuente
authors.forename: M
authors.initials: M
authors.lastname: Carazo
authors.forename: R
authors.initials: R
authors.lastname: Correa
authors.forename: M
authors.initials: M
authors.lastname: Del Río
mesh_terms: D000818:Animals; D002455:Cell Division; D002633:Chemotaxis / Q000187:drug effects; D006168:Guinea Pigs; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000276:immunology; D010587:Phagocytosis / Q000187:drug effects; D013481:Superoxides / Q000378:metabolism; D014810:Vitamin E / Q000008:administration & dosage / Q000276:immunology / Q000494:pharmacology*
chemical_list: D013481:Superoxides; D014810:Vitamin E