title: Role of the outer membrane in the accumulation of quinolones by Serratia marcescens.
abstract: Accumulation of four quinolones by Serratia marcescens was measured fluorometrically. The passage of quinolones through the outer membrane was studied in both lipopolysaccharide-deficient and porin-deficient mutants. The lipopolysaccharide (LPS) layer formed a partially effective barrier for highly hydrophobic quinolones such as nalidixic acid. Quinolones with a low relative hydrophobicity coefficient seemed to pass preferentially through the water-filled Omp3 porin channels. Results were confirmed when Omp3 was cloned in a porin-defective Escherichia coli.
authors.affiliation: Laboratory of Microbiology, University of Barcelona, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Berlanga
authors.forename: N
authors.initials: N
authors.lastname: Ruiz
authors.forename: J
authors.initials: J
authors.lastname: Hernandez-Borrell
authors.forename: T
authors.initials: T
authors.lastname: Montero
authors.forename: M
authors.initials: M
authors.lastname: Viñas
mesh_terms: D042462:4-Quinolones; D000890:Anti-Infective Agents / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005470:Fluorometry / Q000379:methods; D008070:Lipopolysaccharides / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D018272:Porins / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D012706:Serratia marcescens / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*
chemical_list: D042462:4-Quinolones; D000890:Anti-Infective Agents; D008070:Lipopolysaccharides; D018272:Porins; D011994:Recombinant Proteins