Expression of a retinoid-inducible tumor suppressor, Tazarotene-inducible gene-3, is decreased in psoriasis and skin cancer.
Tazarotene-induced gene-3 (TIG-3), isolated from human keratinocytes treated with the retinoic acid receptor-selective retinoid Tazarotene, is homologous to H-rev, a class II tumor suppressor. TIG-3 gene localized to chromosome 11q23, a site of loss of heterozygosity in several malignancies. Retinoids influence epidermal differentiation and are used to treat and prevent skin cancer. Therefore, we studied TIG-3 mRNA expression in psoriasis and in basal and SCCs by in situ hybridization and a quantitative QT-RT-PCR assay. Psoriasis lesions had significantly lower staining (median, 3) than paired normal control skin (median, 4; P = 0.012). TIG-3 mRNA was significantly higher in normal control skin (P = 0.001), in paired adjacent skin (median, 3; P = 0.007), and in overlying epidermis (median, 3.0; P = 0.0001) than in 21 SCC specimens as a group (median, 1.5).
Duvic
M
M
Department of Internal Medical Specialties, M. D. Anderson Cancer Center, Houston, Texas 77030, USA. mduvic@mdanderson.org
Helekar
B
B
Schulz
C
C
Cho
M
M
DiSepio
D
D
Hager
C
C
DiMao
D
D
Hazarika
P
P
Jackson
B
B
Breuer-McHam
J
J
Young
J
J
Clayman
G
G
Lippman
S M
SM
Chandraratna
R A
RA
Robinson
N A
NA
Deucher
A
A
Eckert
R L
RL
Nagpal
S
S
D000368:Aged; D000369:Aged, 80 and over; D002280:Basal Cell Carcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics*; D004817:Epidermis / Q000378:metabolism / Q000502:physiology; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D016147:Genes, Tumor Suppressor; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D011565:Psoriasis / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D018168:Receptors, Retinoic Acid; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000378:metabolism; D012878:Skin Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D012879:Skin Physiological Phenomena
D002352:Carrier Proteins; C437968:PLAAT4 protein, human; D012333:RNA, Messenger; D018168:Receptors, Retinoic Acid