title: An activated O --> N acyl transfer auxiliary: efficient amide-backbone substitution of hindered "difficult" peptides.
abstract: Overcoming the phenomenon known as "difficult" synthetic sequences has been a major goal in solid-phase peptide synthesis for over 30 years. In this work the advantages of amide backbone-substitution in the solid-phase synthesis of "difficult" peptides are augmented by developing an activated N(alpha)()-acyl transfer auxiliary. Apart from disrupting troublesome intermolecular hydrogen-bonding networks, the primary function of the activated N(alpha)()-auxiliary was to facilitate clean and efficient acyl capture of large or beta-branched amino acids and improve acyl transfer yields to the secondary N(alpha)()-amine. We found o-hydroxyl-substituted nitrobenzyl (Hnb) groups were suitable N(alpha)()-auxiliaries for this purpose. The relative acyl transfer efficiency of the Hnb auxiliary was superior to the 2-hydroxy-4-methoxybenzyl (Hmb) auxiliary with protected amino acids of varying size. Significantly, this difference in efficiency was more pronounced between more sterically demanding amino acids. The Hnb auxiliary is readily incorporated at the N(alpha)()-amine during SPPS by reductive alkylation of its corresponding benzaldehyde derivative and conveniently removed by mild photolysis at 366 nm. The usefulness of the Hnb auxiliary for the improvement of coupling efficiencies in the chain-assembly of difficult peptides was demonstrated by the efficient Hnb-assisted Fmoc solid-phase synthesis of a known hindered difficult peptide sequence, STAT-91. This work suggests the Hnb auxiliary will significantly enhance our ability to synthesize difficult polypeptides and increases the applicability of amide-backbone substitution.
authors.affiliation: Centre for Drug Design & Development, The University Of Queensland, Brisbane, Queensland 4072, Australia. Les@crc.dk
authors.forename: L P
authors.initials: LP
authors.lastname: Miranda
authors.forename: W D
authors.initials: WD
authors.lastname: Meutermans
authors.forename: M L
authors.initials: ML
authors.lastname: Smythe
authors.forename: P F
authors.initials: PF
authors.lastname: Alewood
mesh_terms: D000215:Acylation; D000577:Amides / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*
chemical_list: D000577:Amides; D010455:Peptides