A rapid and efficient system of Agrobacterium infection-mediated transient gene expression in rice Oc cells and its application for analysis of the expression and antisense suppression of preprophytosulfokine, a precursor of phytosulfokine-a, encoded by OsPSK gene.
A rapid and efficient system for Agrobacterium infection-mediated transient gene expression in rice has been developed. Using this system, transient expression of preprophytosulfokine, a precursor of phytosulfokine-a, encoded by OsPSK gene was analyzed. The results suggest that the Agrobacterium infection-mediated transient gene expression system is as efficient in rice Oc cells as in tobacco BY-2 cells and might be useful for rapid analysis not only of foreign gene expression, but also of antisense gene suppression.
Yang
H
H
Laboratory of Bioactive Natural Product Chemistry, Graduate School of Bio-Agricultural Sciences, Nagoya University, Chikusa, Japan. hyang@agr.nagoya-u.ac.jp
Morita
A
A
Matsubayashi
Y
Y
Nakamura
K
K
Sakagami
Y
Y
D000199:Actins / Q000235:genetics; D016960:Agrobacterium tumefaciens / Q000235:genetics*; D002478:Cells, Cultured; D018920:Coculture Techniques; D016373:DNA, Antisense; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D017343:Genes, Plant; D005966:Glucuronidase / Q000235:genetics; D007438:Introns; D007700:Kinetics; D012275:Oryza / Q000166:cytology / Q000235:genetics* / Q000378:metabolism*; D036361:Peptide Hormones; D010937:Plant Growth Regulators / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics*; D013489:Suppression, Genetic; D014162:Transfection / Q000379:methods
D000199:Actins; D016373:DNA, Antisense; C100975:PSK-alpha protein, plant; D036361:Peptide Hormones; D010937:Plant Growth Regulators; D010940:Plant Proteins; D011498:Protein Precursors; D005966:Glucuronidase