Comparative studies on cell stimulatory, permeabilizing and toxic effects induced in sensitive and multidrug resistant fungal strains by amphotericin B (AMB) and N-methyl-N-D-fructosyl amphotericin B methyl ester (MFAME).
N-Methyl-N-D-fructosyl-amphotericin B methyl ester (MFAME) is a new derivative of amphotericin B, which is characterised by low toxicity to mammalian cells and good solubility in water of its salts. The antifungal activity and effects of MFAME towards Candida albicans and Saccharomyces cerevisiae multidrug resistant MDR(+) and sensitive MDR(-) strains was compared with those of parent compound. The results obtained indicate that MDR(+) S. cerevisiae was sensitive to MFAME as well as to AMB. MFAME exhibited the same effects on fungal cells studied as parent antibiotic. The two antibiotics, depending on the dose applied induced cell stimulation, K+ efflux, and/or had a toxic effect.
Szlinder-Richert
J
J
Department of Pharmaceutical Technology and Biochemistry, Technical University of Gdańsk, Poland.
Cybulska
B
B
Grzybowska
J
J
Borowski
E
E
Prasad
R
R
D000666:Amphotericin B / Q000031:analogs & derivatives / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects; D018432:Drug Resistance, Multiple; D012441:Saccharomyces cerevisiae / Q000187:drug effects
D000935:Antifungal Agents; C108557:N-methyl-N-fructopyranosylamphotericin B methyl ester; D000666:Amphotericin B