Alteration in dopamine metabolism in the thermoregulatory center of exercising rats.
To examine the role of monoamines and amino acids in thermoregulation, we measured their concentrations in the preoptic area and anterior hypothalamus (PO/AH) in exercising rats, using an in vivo microdialysis technique. Body temperature (Tb) was monitored using a telemetry system. Tb increase by about 1.0 degrees C in the first 15 min of treadmill exercise (10 m/min; for 60 min), and was maintained thereafter at a steady high level possibly due to activation of the heat loss system. The levels of dopamine metabolites (3,4-dihydroxyphenylacetic acid and homovanillic acid) in the PO/AH significantly increased during exercise. However, exercise did not induce an increase in the level of either serotonergic substances (5-hydroxytryptamine and 5-hydroxyindoleacetic acid) or amino acids (aspartate and glutamate). Our data indicate that dopamine breakdown processes in the PO/AH are activated during exercise. Dopamine in the PO/AH may be involved in the heat loss mechanisms for thermoregulation when Tb rises during exercise.
Hasegawa
H
H
Department of Biology, Faculty of Science, Tokyo Metropolitan University, Hachioji, Tokyo, Japan. hasehiro@hiroshima-u.ac.jp
Yazawa
T
T
Yasumatsu
M
M
Otokawa
M
M
Aihara
Y
Y
D000818:Animals; D007025:Anterior Hypothalamic Nucleus / Q000166:cytology / Q000378:metabolism*; D001831:Body Temperature / Q000502:physiology; D001833:Body Temperature Regulation / Q000502:physiology*; D004298:Dopamine / Q000378:metabolism*; D018846:Excitatory Amino Acids / Q000378:metabolism; D008297:Male; D017551:Microdialysis / Q000706:statistics & numerical data; D009434:Neural Pathways / Q000166:cytology / Q000378:metabolism; D010805:Physical Conditioning, Animal / Q000706:statistics & numerical data; D011301:Preoptic Area / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000378:metabolism
D018846:Excitatory Amino Acids; D012701:Serotonin; D004298:Dopamine