title: A microfluidic cartridge to prepare spores for PCR analysis.
abstract: A prototype cartridge system is described that rapidly disrupts Bacillus spores by sonication, adds PCR reagent to the disrupted spores, and dispenses the mixture into a PCR tube. The total time to automatically process the spores in the cartridge and then detect the spore DNA by real-time PCR was 20 min.
authors.affiliation: Cepheid, Sunnyvale, CA 94089, USA.
authors.forename: P
authors.initials: P
authors.lastname: Belgrader
authors.forename: M
authors.initials: M
authors.lastname: Okuzumi
authors.forename: F
authors.initials: F
authors.lastname: Pourahmadi
authors.forename: D A
authors.initials: DA
authors.lastname: Borkholder
authors.forename: M A
authors.initials: MA
authors.lastname: Northrup
mesh_terms: D001407:Bacillus / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods; D013170:Spores / Q000302:isolation & purification