Investigation of mathematical methods for efficient optimisation of aqueous two-phase extraction.
Mathematical strategies were applied to optimise the extraction of recombinant leucine dehydrogenase from E. coli homogenates and endoglucanase 1 from culture filtrates of Trichoderma reesei in polyethylene glycol-phosphate systems. The goal was to test mathematical tools which could facilitate the optimisation procedure in aqueous two-phase systems. A modified simplex approach, the method of steepest ascent and a genetic algorithm were successfully applied and compared. The methods differ in the height of the optimum found, the number of experiments and the time required. The genetic algorithm proved to be an optimisation procedure which can be used well in aqueous two-phase systems. The simplex procedure has to be further improved.
Selber
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Instituts of Enzyme Technology, Heinrich-Heine-Universität Düsseldorf, Jülich, Germany.
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Thömmes
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D000465:Algorithms; D000594:Amino Acid Oxidoreductases / Q000302:isolation & purification*; D002480:Cellulase / Q000302:isolation & purification*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005285:Fermentation; D050803:Leucine Dehydrogenase; D011994:Recombinant Proteins / Q000302:isolation & purification; D014242:Trichoderma / Q000201:enzymology; D014867:Water / Q000737:chemistry
D011994:Recombinant Proteins; D014867:Water; D000594:Amino Acid Oxidoreductases; D050803:Leucine Dehydrogenase; C056374:endoglucanase 1; D002480:Cellulase