Functional parathyroid hormone receptors are present in an umbilical vein endothelial cell line.
Acute parathyroid hormone exposure induces vascular smooth muscle relaxation. In contrast, continuous infusion of parathyroid hormone leads to vasoconstriction and an elevation of blood pressure. Despite the known effects of parathyroid hormone on vascular smooth muscle, possible direct effects on the vascular endothelium have not previously been investigated. Using a human umbilical vein endothelial cell line, we found that parathyroid hormone increased both intracellular calcium and cellular cAMP content in these endothelial cells. Furthermore, exposure of these cells to increasing concentrations of parathyroid hormone stimulated both [(3)H]thymidine incorporation and endothelin-1 secretion. Parathyroid hormone/parathyroid hormone-related peptide receptor mRNA could be detected at low levels in these cells. In summary, these data demonstrate that endothelium-derived cells contain functional parathyroid hormone receptors. The potential physiological role of these receptors remains to be determined.
Isales
C M
CM
Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta 30912, USA. cisales@mail.mcg.edu
Sumpio
B
B
Bollag
R J
RJ
Zhong
Q
Q
Ding
K H
KH
Du
W
W
Rodriguez-Commes
J
J
Lopez
R
R
Rosales
O R
OR
Gasalla-Herraiz
J
J
McCarthy
R
R
Barrett
P Q
PQ
D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D000242:Cyclic AMP / Q000096:biosynthesis; D019332:Endothelin-1 / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005456:Fluorescent Dyes; D016257:Fura-2; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D006801:Humans; D007295:Inositol Phosphates / Q000378:metabolism; D010281:Parathyroid Hormone / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D018016:Receptors, Parathyroid Hormone / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology; D013936:Thymidine / Q000378:metabolism; D014471:Umbilical Veins / Q000166:cytology / Q000378:metabolism*
D019332:Endothelin-1; D005456:Fluorescent Dyes; D007295:Inositol Phosphates; D010281:Parathyroid Hormone; D012333:RNA, Messenger; D018016:Receptors, Parathyroid Hormone; D000242:Cyclic AMP; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D002118:Calcium; D016257:Fura-2; D013936:Thymidine