title: Early steps of polyomavirus entry into cells.
abstract: The mechanism by which murine polyomavirus penetrates cells and arrives at the nucleus, the site of viral replication, is not well understood. Simian virus 40 and JC virus, two closely related members of the polyomavirus subfamily, use caveola- and clathrin-mediated uptake pathways for entry, respectively. The data presented here indicate that compounds that block endocytosis of both caveola- and clathrin-derived vesicles have no effect on polyomavirus infectivity. Polyomavirus does not appear to colocalize with either clathrin light chain or caveolin-1 by immunofluorescence microscopy. Additionally, expression of a dominant-negative form of dynamin I has no effect on polyomavirus uptake and infectivity. Therefore, polyomavirus uptake occurs through a class of uncoated vesicles in a clathrin-, caveolin-1-, and dynamin I-independent manner.
authors.affiliation: Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA. jgilbert@hms.harvard.edu
authors.forename: J M
authors.initials: JM
authors.lastname: Gilbert
authors.forename: T L
authors.initials: TL
authors.lastname: Benjamin
mesh_terms: D016475:3T3 Cells; D000818:Animals; D051242:Caveolin 1; D022461:Caveolins; D002460:Cell Line; D002966:Clathrin / Q000378:metabolism; D018699:Coated Vesicles / Q000378:metabolism; D034284:Dynamin I; D034281:Dynamins; D004705:Endocytosis; D019084:Fluorescent Antibody Technique, Indirect; D020558:GTP Phosphohydrolases / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D011120:Polyomavirus / Q000472:pathogenicity* / Q000648:ultrastructure
chemical_list: C495541:Cav1 protein, mouse; D051242:Caveolin 1; D022461:Caveolins; D002966:Clathrin; D008565:Membrane Proteins; D034284:Dynamin I; D020558:GTP Phosphohydrolases; D034281:Dynamins