Adherence in tissues of immunocompromised mice of a non-mycelium producing strain of Candida albicans.
A non-mycelial strain of wild-type Candida albicans strain 3153 A was produced by repeated subculturing on Sabouraud glucose agar, and maintained on yeast extract-peptone-glucose medium resulting in hydrophobic cells at 26 degrees C and hydrophilic cells at 37 degrees C. The behaviour of cells of this strain was studied in male BALB/c mice, immunocompromised by treatment with cyclophosphamide and cortisone acetate. An ex-vivo assay of cell adherence to tissue sections of liver, spleen, kidney and lymph-nodes was used. The adherence of yeast cells at 26 and 37 degrees C was predominantly produced by hydrophobic cells and was significantly greater in spleen and liver of immunosuppressed mice compared with the organs of control animals. Adhesion was observed in the white and red pulp as well as in the marginal zone of the spleen.
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Department of Microbiology and Immunology, Texas Technical University Health Science Center, Lubbock, USA.
D000818:Animals; D002176:Candida albicans / Q000472:pathogenicity* / Q000502:physiology*; D002177:Candidiasis / Q000382:microbiology*; D002448:Cell Adhesion; D016867:Immunocompromised Host; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D014774:Virulence