title: Microfabricated elastomeric stencils for micropatterning cell cultures.
abstract: Here we present an inexpensive method to fabricate microscopic cellular cultures, which does not require any surface modification of the substrate prior to cell seeding. The method utilizes a reusable elastomeric stencil (i.e., a membrane containing thru holes) which seals spontaneously against the surface. The stencil is applied to the cell-culture substrate before seeding. During seeding, the stencil prevents the substrate from being exposed to the cell suspension except on the hole areas. After cells are allowed to attach and the stencil is peeled off, cellular islands with a shape similar to the holes remain on the cell-culture substrate. This solvent-free method can be combined with a wide range of substrates (including biocompatible polymers, homogeneous or nonplanar surfaces, microelectronic chips, and gels), biomolecules, and virtually any adherent cell type.
authors.affiliation: Center for Engineering in Medicine, Massachusetts General Hospital and Harvard Medical School, 55 Fruit St., Bigelow 1401, Boston, MA 02114, USA.
authors.forename: A
authors.initials: A
authors.lastname: Folch
authors.forename: B H
authors.initials: BH
authors.lastname: Jo
authors.forename: O
authors.initials: O
authors.lastname: Hurtado
authors.forename: D J
authors.initials: DJ
authors.lastname: Beebe
authors.forename: M
authors.initials: M
authors.lastname: Toner
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000295:instrumentation*; D020099:Coated Materials, Biocompatible; D004129:Dimethylpolysiloxanes; D005347:Fibroblasts; D006801:Humans; D012828:Silicones
chemical_list: D020099:Coated Materials, Biocompatible; D004129:Dimethylpolysiloxanes; D012828:Silicones; C013830:baysilon