Effect of lead, cadmium and zinc on the activity of enamel matrix proteinases in vitro.
Environmental contamination with heavy metals leads to their uptake by the body. Dental tissues are known to harbor metals in concentrations related to the exposure at the time of dentin or enamel formation. Several enzymes involved in the metabolism of extracellular matrix components have been shown to be inhibited by excess metal ions. Enamel matrix proteinases seem to play a central role in the maturation of dental enamel, and inhibition of these enzymes by metals could interfere with amelogenesis. We have investigated the effect of lead, cadmium and zinc on the activity of enamel matrix proteinases using a colorimetric assay, casein and gelatin zymography, and an assay of enamel protein degradation in vitro. All three metals inhibited the proteolysis, as shown by the three methods employed, in concentrations as low as 110 microM. This study showed that contaminant metals can inhibit proteinolysis when a crude enamel extract containing enamel proteases is tested in vitro. Therefore, the presence of heavy metals in enamel matrix could interfere with amelogenesis due to alteration in normal proteolysis.
Gerlach
R F
RF
Department of Morphology, Dental School of Piracicaba, University of Campinas, São Paulo, Brazil.
de Souza
A P
AP
Cury
J A
JA
Line
S R
SR
D000566:Amelogenesis / Q000187:drug effects*; D000704:Analysis of Variance; D000818:Animals; D002104:Cadmium / Q000493:pharmacokinetics / Q000633:toxicity; D003124:Colorimetry; D003743:Dental Enamel / Q000187:drug effects* / Q000201:enzymology* / Q000378:metabolism; D003746:Dental Enamel Proteins / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D010450:Endopeptidases / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D066298:In Vitro Techniques; D007854:Lead / Q000493:pharmacokinetics / Q000633:toxicity; D019216:Metals, Heavy / Q000493:pharmacokinetics / Q000633:toxicity*; D011480:Protease Inhibitors / Q000378:metabolism; D051381:Rats; D015032:Zinc / Q000493:pharmacokinetics / Q000633:toxicity
D003746:Dental Enamel Proteins; D016326:Extracellular Matrix Proteins; D019216:Metals, Heavy; D011480:Protease Inhibitors; D002104:Cadmium; D007854:Lead; D010450:Endopeptidases; D015032:Zinc