Baculoviral display of functional scFv and synthetic IgG-binding domains.
Viral vectors displaying specific ligand binding moities such as scFv fragments or intact antibodies hold promise for the development of targeted gene therapy vectors. In this report we describe baculoviral vectors displaying either functional scFv fragments or the synthetic Z/ZZ IgG binding domain derived from protein A. Display on the baculovirus surface was achieved via fusion of the scFv fragment or Z/ZZ domain to the N-terminus of gp64, the major envelope protein of the Autographa californica nuclear polyhedrosis virus, AcNPV. As examples of scFv fragments we have used a murine scFv specific for the hapten 2-phenyloxazolone and a human scFv specific for carcinoembryonic antigen. In principle, the Z/ZZ IgG binding domain displaying baculoviruses could be targeted to specific cell types via the binding of an appropriate antibody. We envisage applications for scFv and Z/ZZ domain displaying baculoviral vectors in the gene therapy field.
Mottershead
D G
DG
Department of Biosciences, University of Helsinki, Helsinki, FIN-00014, Finland.
Alfthan
K
K
Ojala
K
K
Takkinen
K
K
Oker-Blom
C
C
D000818:Animals; D000918:Antibody Specificity; D016367:Baculoviridae / Q000235:genetics* / Q000378:metabolism; D001666:Binding Sites, Antibody / Q000235:genetics / Q000276:immunology*; D015153:Blotting, Western; D002272:Carcinoembryonic Antigen / Q000235:genetics / Q000276:immunology; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D006241:Haptens / Q000235:genetics / Q000276:immunology; D006801:Humans; D007128:Immunoglobulin Fragments / Q000235:genetics / Q000276:immunology*; D007074:Immunoglobulin G / Q000235:genetics / Q000276:immunology*; D051379:Mice; D010081:Oxazolone / Q000031:analogs & derivatives / Q000276:immunology; D019151:Peptide Library; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D018411:Spodoptera / Q000821:virology; D014760:Viral Fusion Proteins / Q000235:genetics / Q000378:metabolism
D002272:Carcinoembryonic Antigen; D006241:Haptens; D007128:Immunoglobulin Fragments; D007074:Immunoglobulin G; D019151:Peptide Library; D011993:Recombinant Fusion Proteins; D014760:Viral Fusion Proteins; C018361:2-phenyloxazolone; D010081:Oxazolone