title: Crystallization and X-ray diffraction analysis of peroxisomal Delta3-Delta2-enoyl-CoA isomerase from Saccharomyces cerevisiae.
abstract: The purification, crystallization and X-ray diffraction analysis of Saccharomyces cerevisiae Delta(3)-Delta(2)-enoyl-CoA isomerase is described. Delta(3)-Delta(2)-Enoyl-CoA isomerase is a member of the hydratase/isomerase protein family and is an auxiliary enzyme required for the beta-oxidation of unsaturated fatty acids. It is a hexameric enzyme consisting of six identical 32 kDa subunits of 280 residues each. In crystallization trials three crystal forms were obtained, with tetragonal and hexagonal lattices. A 2.5 A data set was collected from the unliganded hexagonal crystals with an R(merge) of 6.6%. The crystal, with unit-cell parameters a = 116.0, b = 116.0, c = 122.9 A, is likely to have P6(3)22 symmetry.
authors.affiliation: Biocenter Oulu and Department of Biochemistry, University of Oulu, BOX 3000, FIN-90014 Oulun Yliopisto, Finland.
authors.forename: A M
authors.initials: AM
authors.lastname: Mursula
authors.forename: D M
authors.initials: DM
authors.lastname: van Aalten
authors.forename: Y
authors.initials: Y
authors.lastname: Modis
authors.forename: J K
authors.initials: JK
authors.lastname: Hiltunen
authors.forename: R K
authors.initials: RK
authors.lastname: Wierenga
mesh_terms: D019748:Carbon-Carbon Double Bond Isomerases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D064006:Dodecenoyl-CoA Isomerase; D004926:Escherichia coli / Q000235:genetics; D008970:Molecular Weight; D020675:Peroxisomes / Q000201:enzymology; D020836:Protein Structure, Quaternary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
chemical_list: D011994:Recombinant Proteins; D019748:Carbon-Carbon Double Bond Isomerases; D064006:Dodecenoyl-CoA Isomerase