title: Total tumor cell elimination with minimum damage to normal tissues in musculoskeletal sarcomas following photodynamic therapy with acridine orange.
abstract: Acridine orange (AO) has unique biological actions enabling tumor visualization (fluorovisualization) and a strong cytocidal effect (photodynamic therapy: AO-PDT) under illumination with blue light. Accordingly, in this study, we attempted to develop a new surgical technique for total tumor cell elimination using these photodynamic reactions with AO in a mouse osteosarcoma model. The results showed that local tumor recurrence was significantly inhibited (23%) in the group treated with curettage under fluorovisualization and AO-PDT, compared to that (80%) in the control group treated with curettage alone under ordinary light. Therefore, we concluded that the combination of curettage under fluorovisualization and AO-PDT may be useful for total tumor cell elimination with minimum damage to normal tissue in musculoskeletal sarcomas.
authors.affiliation: Orthopedic Surgery, Kyoto Prefectural University of Medicine, Kyoto, Japan. kusu@basic.kpu-m.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Kusuzaki
authors.forename: K
authors.initials: K
authors.lastname: Aomori
authors.forename: T
authors.initials: T
authors.lastname: Suginoshita
authors.forename: G
authors.initials: G
authors.lastname: Minami
authors.forename: H
authors.initials: H
authors.lastname: Takeshita
authors.forename: H
authors.initials: H
authors.lastname: Murata
authors.forename: S
authors.initials: S
authors.lastname: Hashiguchi
authors.forename: T
authors.initials: T
authors.lastname: Ashihara
authors.forename: Y
authors.initials: Y
authors.lastname: Hirasawa
mesh_terms: D000165:Acridine Orange / Q000627:therapeutic use*; D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D001859:Bone Neoplasms / Q000188:drug therapy*; D003475:Curettage; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009140:Musculoskeletal Diseases / Q000188:drug therapy; D009153:Mutagens / Q000627:therapeutic use; D012516:Osteosarcoma / Q000188:drug therapy*; D010778:Photochemotherapy; D016896:Treatment Outcome
chemical_list: D000970:Antineoplastic Agents; D009153:Mutagens; D000165:Acridine Orange