title: Adenosine triphosphate mobilizes cytosolic calcium and modulates ionic currents in mouse taste receptor cells.
abstract: By using photometry and the patch clamp technique, we identified P(2Y)-like receptors in mouse taste receptor cells (TRCs) and found them to be coupled to Ca(2+) mobilization and ionic current modulation. Particularly, adenosine triphosphate (ATP) and the P(2Y) agonist 2-methylthio-ATP increased intracellular Ca(2+) by stimulating the phosphoinositide pathway, whereas beta, gamma-methylene-D-ATP, a P(2X) agonist, was ineffective. In a distinctive TRC subpopulation, ATP closed Ca(2+) channels. This regulation may underlie the negative feedback tuning neurotransmitter release. By mobilizing intracellular Ca(2+), ATP activated Ca(2+)-dependent Cl(-) channels, the intracellular event that may universally occur upon taste stimulation triggering IP(3) formation and Ca(2+) release in the TRC cytoplasm.
authors.affiliation: Institute of Cell Biophysics, Russian Academy of Sciences, Moscow Region, 142290, Pushchino, Russia.
authors.forename: Y V
authors.initials: YV
authors.lastname: Kim
authors.forename: Y V
authors.initials: YV
authors.lastname: Bobkov
authors.forename: S S
authors.initials: SS
authors.lastname: Kolesnikov
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects* / Q000502:physiology; D003600:Cytosol / Q000187:drug effects* / Q000378:metabolism; D007473:Ion Channels / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D011983:Receptors, Purinergic / Q000187:drug effects / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D013650:Taste Buds / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D007473:Ion Channels; D011983:Receptors, Purinergic; D000255:Adenosine Triphosphate; D002118:Calcium