Detection of antigen-specific human serum proteins related to the T-cell receptor in infectious disease and in an immune response to milk proteins or chemicals.
A monoclonal IgG2 antibody, MG3C9-1 A12, was prepared by immunization of mice with human serum Cohn Fraction III proteins enriched for TCR Ca+ proteins. MG3C9-1 A12 bound to Mr 28,000, antigen-specific TCR Ca+, beta-, and TCR Ca+, beta+ serum proteins associated with TGF-beta1, 2. The IgG2 monoclonal antibody also bound to T-lymphocyte proteins but did not bind to B lymphocyte proteins, human albumin, IgM, IgG, IgA, or TGF-beta1, 2, 3 immunogenic peptides. Monoclonal MG3C9-1 A12 detected TCR-related proteins specific for filarial extract, milk proteins, or benzoic acid in the sera of individuals with chronic or asymptomatic filariasis, milk intolerance, or sensitivity to toluene, respectively. TCR-related serum proteins were also detected intracellularly in mononuclear cells in frozen sections of ileum from a patient with milk intolerance and reactive mesenteric lymph nodes from a patient with a gastric ulcer. The results suggest that antigen-specific TCR-related serum proteins may be elevated during an immune response to oral, environmental, or infectious stimuli.
Little
C H
CH
Specialist Allergist, Mt. Waverly, Melbourne, Victoria, Australia 3149.
Georgiou
G M
GM
Fey
G
G
Ravindran
B
B
Levine
J
J
Ogedegbe
H
H
Yamase
H
H
Cone
R E
RE
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology; D019817:Benzoic Acid / Q000008:administration & dosage / Q000276:immunology; D001798:Blood Proteins / Q000032:analysis / Q000276:immunology*; D004781:Environmental Exposure; D000939:Epitopes / Q000276:immunology; D005260:Female; D005368:Filariasis / Q000276:immunology*; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology*; D007082:Ileum / Q000276:immunology / Q000473:pathology; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology; D051379:Mice; D008875:Middle Aged; D016269:Milk Hypersensitivity / Q000276:immunology; D008894:Milk Proteins / Q000276:immunology*; D010437:Peptic Ulcer / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology; D014050:Toluene / Q000008:administration & dosage / Q000276:immunology
D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D001798:Blood Proteins; C028500:Cohn fraction III; D000939:Epitopes; D008894:Milk Proteins; D011948:Receptors, Antigen, T-Cell; D014050:Toluene; D019817:Benzoic Acid