[Use of cryopreserved sperm in the microagglutination test].
OBJECTIVE
The aim of this study was to find out the behavior of sperm cryopreserved in microagglutination test (TAT).


METHODS
TAT using native sperm pertains to the screening methods in spermagglutinating antibodies. Spermatozoa cryopreserved in egg-yolk medium, just in seminal plasma or in the commercial made medium--Medi-Cult (Denmark) were used in this study.


METHODS
Department of Gynaecology and Obstetrics, Institute of Clinical Immunology and Allergology Medical Faculty of Charles University, Pilsen.


RESULTS
367 tests were performed together. It includes 108 examinations with native spermatozoa, 59 with spermcryopreserved in egg-yolk medium, 109 with spermcryopreserved just in seminal plasma and 91 in Medi-Cult medium. The correspondance of results with native and cryopreserved sperm was found in 24% for egg-yolk medium, in 69% for seminal plasma and in 77% for commercial medium Medi-Cult.


CONCLUSIONS
The results show that spermcryopreservation in Medi-Cult medium or just in the seminal plasma are able to be used in TAT for detection of spermagglutinating antibodies.
Seifertová
P
P
Gynekologicko-porodnická klinika LF UK a FN v Plzni.
Ulcová-Gallová
Z
Z
Panzner
P
P
Krizanovská
K
K
Rokyta
Z
Z
D000906:Antibodies / Q000032:analysis; D015925:Cryopreservation / Q000379:methods; D006801:Humans; D008297:Male; D013073:Sperm Agglutination; D013094:Spermatozoa / Q000276:immunology
D000906:Antibodies