Selective isolation and distribution of Actinobispora strains in soil.
A simplified enrichment method for selective isolation of Actinobispora strains from soil is described. Actinobispora spores were tolerant to dry-heat treatment at 110 degrees C for 15 min. Actinobispora was more resistant to 1 microgram/mL leucomycin, 1 microgram/mL novobiocin, and 0.5 microgram/mL tunicamycin than Streptomyces dominant in soil, which prevents selective isolation of Actinobispora. Percentages of Actinobispora colonies on the isolation plate were increased by addition of antibiotics and dry-heat treatment of the soil samples. By combining the techniques described above, this genus was isolated from 105 out of 574 soil samples (18% of the samples tested). It was recovered from the soil samples with pH values ranging 5.0 to 8.9, and 78% of strains were isolated from neutral soil (pH 6.0-8.0). A number of Actinobispora strains were isolated from various soils around the world. Actinobispora strains are widely distributed in the world at relatively high frequency.
Suzuki
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S
Basic Technology Department, Tanabe Seiyaku Co., Ltd., Saitama, Japan. s-suzuki@tanabe.co.jp
Okuda
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T
Komatsubara
S
S
D000192:Actinomycetales / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001431:Bacteriological Techniques / Q000379:methods; D002122:Calcium Chloride / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D006358:Hot Temperature; D008826:Microbial Sensitivity Tests; D011135:Polysaccharides, Bacterial / Q000378:metabolism; D012988:Soil Microbiology
D000900:Anti-Bacterial Agents; D003470:Culture Media; D011135:Polysaccharides, Bacterial; C048288:gellan gum; D002122:Calcium Chloride