Detection of antigen-specific cytotoxic T lymphocytes: significance for investigative dermatology.
Novel recombinant tetrameric complexes of HLA class I molecules allow the direct visualization of antigen-specific CD8+ T cells using flow cytometry. By facilitating the quantification, isolation and phenotypic analysis of CD8+ T cells, the use of HLA tetramers has extended our understanding of the role of cellular immunity in various disease settings. Recently the technique has also been applied to the study of cutaneous disease and provides insights into mechanisms of dermatopathology.
Ogg
G S
GS
MRC Human Immunology Unit, Institute of Molecular Medicine, Oxford, UK. gogg@molbiol.ox.ac.uk
D016827:CD8 Antigens / Q000032:analysis / Q000276:immunology; D000939:Epitopes / Q000032:analysis / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D008280:Magnetics; D008545:Melanoma / Q000276:immunology; D012878:Skin Neoplasms / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014820:Vitiligo / Q000276:immunology
D016827:CD8 Antigens; D000939:Epitopes