Nucleotide-evoked ion transport and [Ca(2+)](i) changes in normal and hyperhidrotic human sweat gland cells.
Apical and basolateral application of ATP and UTP evoked [Ca(2+)](i) and short circuit current (Isc) increases in normal and hyperhidrotic human eccrine sweat gland cells grown into functionally polarised epithelia on permeable supports. Basolateral application to hyperhidrotic cells exhibited a markedly greater increase in Isc than in normal cells. Hyperhidrotic cells also demonstrated differences from the normal in [Ca(2+)](i) and Isc responses to ATP when pre-treated with thapsigargin. The data demonstrate the presence of apical and basolateral receptors that allow nucleotides to increase [Ca(2+)](i) and Isc. The results suggest that changes from the normal in transepithelial ion transport contribute to the characteristic excessive fluid production of hyperhidrotic sweat glands.
Bovell
D L
DL
School of Biological and Biomedical Sciences, Glasgow Caledonian University, G4 0BA, Glasgow, UK. d.bovell@gcal.ac.uk
Clunes
M T
MT
Elder
H Y
HY
Wong
C H
CH
Ko
W H
WH
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D016764:Cell Polarity; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D006801:Humans; D006945:Hyperhidrosis / Q000378:metabolism* / Q000503:physiopathology; D017136:Ion Transport / Q000187:drug effects; D008564:Membrane Potentials / Q000187:drug effects; D013545:Sweat Glands / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D019284:Thapsigargin / Q000494:pharmacology; D014544:Uridine Triphosphate / Q000494:pharmacology*
D019284:Thapsigargin; D000255:Adenosine Triphosphate; D002118:Calcium; D014544:Uridine Triphosphate