title: An NMR spectroscopic identity test for the control of the capsular polysaccharide from Haemophilus influenzae type b.
abstract: We describe the use of Nuclear Magnetic Resonance (NMR) spectroscopy to control the identity of purified bulk capsular polysaccharide [called poly(ribosylribitolphosphate) or PRP] from Haemophilus influenzae type b (Hib), and derivatised forms, used in the production of Hib polysaccharide-protein conjugate vaccines. We describe the approaches we have developed to validate this test.
authors.affiliation: Laboratory for Molecular Structure, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Herts, EN6 3QG, UK.
authors.forename: X
authors.initials: X
authors.lastname: Lemercinier
authors.forename: C
authors.initials: C
authors.lastname: Jones
mesh_terms: D018073:Haemophilus Vaccines / Q000737:chemistry / Q000592:standards*; D006193:Haemophilus influenzae / Q000145:classification / Q000276:immunology*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D011135:Polysaccharides, Bacterial / Q000276:immunology* / Q000302:isolation & purification*; D011786:Quality Control; D012680:Sensitivity and Specificity; D018074:Vaccines, Conjugate / Q000737:chemistry / Q000592:standards*
chemical_list: D018073:Haemophilus Vaccines; D011135:Polysaccharides, Bacterial; D018074:Vaccines, Conjugate