Selective uptake by cancer cells of liposomes coated with polysaccharides bearing 1-aminolactose.
We investigated the selective uptake of liposomes chemically modified by polysaccharides-cholesterol derivatives with 1-aminolactose (lactose) in two human hepatoma cell lines (HUH7 and Alexander), a human colon cancer cell line (FCC) and a human lung cancer cell line (KNS). The uptakes of the labeled liposomes alone (conventional liposomes), those with cholesterol pullulan (CHP) and with lactose (lactose CHP) were compared in four cancer cells and normal rat hepatocytes after 3 hours of incubation. The radioactivities of the lactose CHP were 4.4, 4, 3.4 and 4.4 times greater than those of CHP in HuH7, Alexander, FCC and KNS cells, respectively, after 3 hours of incubation. All the above differences were statistically significant (p < 0.01). No statistically significant differences were seen in the case of hepatocytes. Thus, cancer cells have a common affinity with lactose CHP liposomes, however, these mechanisms appear to have no connection with the galactose-specific asialoglycoprotein receptors of hepatocytes.
Matsukawa
S
S
Department of Surgery II, Nagasaki University School of Medicine, Japan.
Yamamoto
M
M
Ichinose
K
K
Ohata
N
N
Ishii
N
N
Kohji
T
T
Akiyoshi
K
K
Sunamoto
J
J
Kanematsu
T
T
D000818:Animals; D002784:Cholesterol / Q000493:pharmacokinetics*; D005936:Glucans / Q000493:pharmacokinetics*; D006801:Humans; D007444:Inulin / Q000493:pharmacokinetics; D007785:Lactose / Q000493:pharmacokinetics*; D008081:Liposomes / Q000493:pharmacokinetics*; D008099:Liver / Q000378:metabolism; D008113:Liver Neoplasms / Q000378:metabolism; D008297:Male; D009369:Neoplasms / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014407:Tumor Cells, Cultured
D005936:Glucans; D008081:Liposomes; C009109:pullulan; D007444:Inulin; D002784:Cholesterol; D007785:Lactose