Identification of a plasmid-borne chloramphenicol-florfenicol resistance gene in Staphylococcus sciuri.
The 16.5-kbp plasmid pSCFS1 from Staphylococcus sciuri mediated combined resistance to chloramphenicol and florfenicol. The gene responsible for this resistance property, cfr, was cloned and sequenced. The amino acid sequence of the Cfr protein revealed no homology to known acetyltransferases or efflux proteins involved in chloramphenicol and/or florfenicol resistance or to other proteins whose functions are known.
Schwarz
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Institut für Tierzucht und Tierverhalten der Bundesforschungsanstalt für Landwirtschaft Braunschweig (FAL), Celle, Germany. schwarz@ktf.fal.de
Werckenthin
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Kehrenberg
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D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D002701:Chloramphenicol / Q000494:pharmacology; D002702:Chloramphenicol Resistance / Q000235:genetics*; D004352:Drug Resistance, Microbial; D018432:Drug Resistance, Multiple / Q000235:genetics*; D008969:Molecular Sequence Data; D011815:R Factors / Q000235:genetics*; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D013210:Staphylococcus / Q000187:drug effects / Q000235:genetics*; D013839:Thiamphenicol / Q000031:analogs & derivatives* / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; C413412:CFR protein, Staphylococcus sciuri; D002701:Chloramphenicol; C035534:florfenicol; D013839:Thiamphenicol