Manifestation of thrombocytopenia in dengue-2-virus-infected mice.
Dengue virus infection causes dengue fever, dengue haemorrhagic fever and dengue shock syndrome. No animal model is available that mimics these clinical manifestations. In this study, the establishment is reported of a murine model for dengue virus infection that resembles the thrombocytopenia manifestation. Dengue-2 virus (dengue virus type 2) can infect murine cells either in vitro (primary cell culture) or in vivo. Viraemia detected by RT-PCR was found transiently at 2 days after intravenous injection of dengue-2 virus. Transient thrombocytopenia developed at 10-13 days after primary or secondary infection. Anti-platelet antibody was generated after dengue-2 virus infection. There was strain variation in dengue-2 virus infection; the A/J strain was more sensitive than BALB/c or B6 mice. This dengue-2-virus-infected mouse system accompanied by thrombocytopenia and anti-platelet antibody will be a valuable model to study the pathogenicity of dengue virus infection.
Huang
Kao-Jean
KJ
Department of Microbiology & Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Republic of China1.
Li
Shu-Yi J
SJ
Department of Microbiology & Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Republic of China1.
Chen
Shiour-Ching
SC
Department of Microbiology & Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Republic of China1.
Liu
Hsiao-Sheng
HS
Department of Microbiology & Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Republic of China1.
Lin
Yee-Shin
YS
Department of Microbiology & Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Republic of China1.
Yeh
Trai-Ming
TM
Department of Microbiology & Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Republic of China1.
Liu
Ching-Chuan
CC
Department of Microbiology & Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Republic of China1.
Lei
Huan-Yao
HY
Department of Microbiology & Immunology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Republic of China1.
D000818:Animals; D002478:Cells, Cultured; D003715:Dengue / Q000150:complications*; D003716:Dengue Virus / Q000472:pathogenicity; D004195:Disease Models, Animal; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013921:Thrombocytopenia / Q000821:virology*; D014766:Viremia / Q000150:complications