Molecular cloning and characterization of FX3, a novel zinc-finger protein.
In a screen for cyclin G1 interacting proteins using the yeast two-hybrid system, we identified a cDNA clone encoding a novel zinc-finger protein. In addition to a C-terminal zinc-finger domain, the 18 kDa protein, designated FX3, contains a bipartite nuclear targeting sequence and a number of putative protein kinase phosphorylation sites. The physical interaction of FX3 with cyclin G1 was confirmed in vitro by co-immunoprecipitation. Further studies demonstrated that the zinc-finger domain is not required for the observed interaction between FX3 and cyclin G1. FX3 is an essential gene expressed in numerous human tissues, with the highest levels observed in the liver.
Xu
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F
Department of Molecular Pharmacology and Toxicology, School of Pharmacy, University of Southern California, Los Angeles, CA, USA.
Wang
Y
Y
Hall
F L
FL
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D003001:Cloning, Molecular; D056746:Cyclin G; D056766:Cyclin G1; D016213:Cyclins / Q000235:genetics / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D006367:HeLa Cells / Q000737:chemistry; D006801:Humans; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D020319:Oligodeoxyribonucleotides, Antisense / Q000235:genetics / Q000494:pharmacology; D009928:Organ Specificity; D017434:Protein Structure, Tertiary; D051381:Rats; D017386:Sequence Homology, Amino Acid; D016335:Zinc Fingers / Q000235:genetics* / Q000502:physiology
C534702:CCNG1 protein, human; C534704:Ccng1 protein, rat; D056746:Cyclin G; D056766:Cyclin G1; D016213:Cyclins; D018076:DNA, Complementary; C417362:FX3 protein, human; D009124:Muscle Proteins; D020319:Oligodeoxyribonucleotides, Antisense