Polymorphisms of the beta2-adrenergic receptor gene (ADRB2) in relation to cardiovascular risk factors in men.
OBJECTIVE
To investigate the effect of polymorphisms in codon 16 (Arg16Gly) and codon 27 (Gln27Glu) of the beta2-adrenergic receptor gene (ADRB2) on anthropometric, endocrine, metabolic and haemodynamic variables.


METHODS
A cross-sectional study.


METHODS
A subgroup of 284 Swedish men from a population sample of 1040 at the age of 51 years.


METHODS
Genotype examination of ADRB2 polymorphisms in codon 16 and codon 27 with polymerase chain reaction and restriction fragment length polymorphism. Anthropometric measurements included body mass index, waist-to-hip ratio and abdominal sagittal diameter. Endocrine measurements included blood levels of testosterone, insulin-like growth factor I, and leptin plus salivary cortisol. Overnight fasting values of serum insulin, blood glucose, triglycerides, total, low and high density lipoprotein cholesterol, as well as blood pressure and resting heart rate, were also determined.


RESULTS
Polymorphisms were frequent in both codon 16 and codon 27. The Arg16Gly genotype showed significant relationships to elevated central distribution of body fat and to systolic blood pressure, whilst the Glu27Glu genotype was associated with elevated leptin and triglyceride levels but not to other measurements, including obesity variables.


CONCLUSIONS
We conclude that only a few cardiovascular risk factors are associated with DNA sequence variation in the ADRB2 in Swedish men.
Rosmond
R
R
Department of Heart and Lung Diseases, Göteborg University, Göteborg, Sweden. rolandrosmond@hotmail.com
Ukkola
O
O
Chagnon
M
M
Bouchard
C
C
Björntorp
P
P
D000483:Alleles; D000886:Anthropometry; D001794:Blood Pressure / Q000235:genetics; D002318:Cardiovascular Diseases / Q000235:genetics; D016009:Chi-Square Distribution; D003062:Codon; D015331:Cohort Studies; D003430:Cross-Sectional Studies; D005838:Genotype; D006339:Heart Rate; D006801:Humans; D020738:Leptin / Q000097:blood; D015810:Linkage Disequilibrium; D008055:Lipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000453:epidemiology / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D018343:Receptors, Adrenergic, beta-2 / Q000235:genetics*; D012307:Risk Factors; D013548:Sweden / Q000453:epidemiology
D003062:Codon; D020738:Leptin; D008055:Lipids; D018343:Receptors, Adrenergic, beta-2