title: Mapping and determination of the cDNA sequence of the Erc gene preferentially expressed in renal cell carcinoma in the Tsc2 gene mutant (Eker) rat model.
abstract: The Eker rat develops hereditary renal carcinomas (RCs) due to two hit mutations of the tumor suppressor gene, Tsc2. We previously identified using representational difference analysis (RDA), four genes that were expressed more abundantly in an Eker rat RC cell line than in normal kidney tissue. One gene, Erc (expressed in renal carcinoma) showed sequence homology to the mouse and human megakaryocyte potentiating factor (MPF)/mesothelin gene. The present study determines the full sequence of the cDNA and the exon-intron structure of the rat Erc gene and maps its locus in the chromosome by fluorescence in situ hybridization. Rat Erc and its human homologue were localized in chromosomes 10q12-21 and 16p13.3, respectively, both of which coincided with the locus of the Tsc2/TSC gene. We also found that Erc was expressed at higher levels in primary RCs compared with the normal kidney of the Eker rat. Erc may be related to carcinogenesis in the Tsc2 gene mutant (Eker) rat model.
authors.affiliation: Department of Experimental Pathology, Cancer Institute, Tokyo, 170-8455, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Yamashita
authors.forename: M
authors.initials: M
authors.lastname: Yokoyama
authors.forename: E
authors.initials: E
authors.lastname: Kobayashi
authors.forename: S
authors.initials: S
authors.lastname: Takai
authors.forename: O
authors.initials: O
authors.lastname: Hino
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002292:Carcinoma, Renal Cell / Q000235:genetics*; D002885:Chromosomes, Human, Pair 16 / Q000235:genetics; D003001:Cloning, Molecular; D004195:Disease Models, Animal; D005091:Exons / Q000235:genetics; D058851:GPI-Linked Proteins; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007438:Introns / Q000235:genetics; D007680:Kidney Neoplasms / Q000235:genetics*; D008562:Membrane Glycoproteins / Q000737:chemistry; D000090204:Mesothelin; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D015513:Oncogene Proteins / Q000737:chemistry / Q000235:genetics*; D020161:Physical Chromosome Mapping; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012097:Repressor Proteins / Q000235:genetics*; D016415:Sequence Alignment; D000077005:Tuberous Sclerosis Complex 2 Protein; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins
chemical_list: C413325:Erc protein, human; D058851:GPI-Linked Proteins; C000715527:MSLN protein, human; D008562:Membrane Glycoproteins; C000715528:Msln protein, mouse; C000715529:Msln protein, rat; D015513:Oncogene Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins; C000624653:TSC2 protein, human; C000624654:Tsc2 protein, mouse; C000624655:Tsc2 protein, rat; D000077005:Tuberous Sclerosis Complex 2 Protein; D025521:Tumor Suppressor Proteins; D000090204:Mesothelin