title: An improved rapid procedure for fluorescence in situ hybridization that is applicable to intraoperative cancer cytodiagnosis.
abstract: Fluorescence in situ hybridization (FISH) is among the most simple and useful methods for detecting numerical and structural aberration of chromosomes but it requires 12-24 h to complete. We devised a rapid FISH method that can be performed within 2 h. Here we describe the technique, which we have found to be extreme simple and as sensitive and specific as standard FISH, making it highly suitable for clinical use.
authors.affiliation: Department of Digestive Surgery, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Hirokohji, Kawaramachi-tori, Kamigyo-ku, Kyoto, Japan. koide@iwa.att.ne.jp
authors.forename: K
authors.initials: K
authors.lastname: Koide
authors.forename: C
authors.initials: C
authors.lastname: Sakakura
authors.forename: A
authors.initials: A
authors.lastname: Hagiwara
authors.forename: T
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: H
authors.initials: H
authors.lastname: Yamagishi
authors.forename: T
authors.initials: T
authors.lastname: Abe
authors.forename: J
authors.initials: J
authors.lastname: Inazawa
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D022981:Allelic Imbalance; D002869:Chromosome Aberrations; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics; D002887:Chromosomes, Human, Pair 18 / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics / Q000473:pathology; D003581:Cytodiagnosis; D003937:Diagnosis, Differential; D004938:Esophageal Neoplasms / Q000235:genetics / Q000473:pathology; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000235:genetics / Q000473:pathology*; D012680:Sensitivity and Specificity; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology; D013997:Time Factors; D020298:U937 Cells; D014998:Y Chromosome / Q000235:genetics