Anaerobic degradation of ethylbenzene and toluene in denitrifying strain EbN1 proceeds via independent substrate-induced pathways.
Denitrifying strain EbN1 utilizes either ethylbenzene or toluene as the sole source of organic carbon under strictly anoxic conditions. When cells were grown on ethylbenzene, 1-phenylethanol and acetophenone were detected in the culture supernatant. However, these two compounds were not observed when cells were grown on benzoate. Growth on ethylbenzene, 1-phenylethanol, or acetophenone strictly depended on the presence of CO2, whereas growth on benzoate did not. These results suggest that strain EbN1 degrades ethylbenzene via 1-phenylethanol and acetophenone as intermediates, and that acetophenone is subsequently carboxylated. In suspensions of benzoate-grown cells, induction was required for degradation of ethylbenzene, 1-phenylethanol, and acetophenone. Induction was also required for toluene-grown cells to gain activity to degrade ethylbenzene, and, conversely, for ethylbenzene-grown cells to degrade toluene. In accordance with our findings from these studies, two-dimensional gel electrophoretic analysis of extracts of cells grown on benzoate, acetophenone, ethylbenzene, or toluene showed that a number of substrate-specific proteins were induced in strain EbN1. Growth on toluene or ethylbenzene induced a distinct set of proteins. However, some of the induced proteins in ethylbenzene or acetophenone grown cells were identical. This agrees with the finding that acetophenone is an intermediate in the degradation of ethylbenzene.
Champion
K M
KM
Max-Planck-Institut für Marine Mikrobiologie, Bremen, Germany.
Zengler
K
K
Rabus
R
R
D000098:Acetophenones / Q000378:metabolism; D000222:Adaptation, Physiological; D000595:Amino Acid Sequence; D000693:Anaerobiosis; D001426:Bacterial Proteins / Q000032:analysis; D001555:Benzene Derivatives / Q000378:metabolism*; D020562:Betaproteobacteria / Q000254:growth & development / Q000378:metabolism* / Q000502:physiology; D001673:Biodegradation, Environmental; D002245:Carbon Dioxide; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D006841:Hydrocarbons, Aromatic / Q000378:metabolism; D008969:Molecular Sequence Data; D017421:Sequence Analysis; D014050:Toluene / Q000378:metabolism*
D000098:Acetophenones; D001426:Bacterial Proteins; D001555:Benzene Derivatives; D006841:Hydrocarbons, Aromatic; D002245:Carbon Dioxide; D014050:Toluene; C004912:ethylbenzene