Steroid profiles determined by capillary electrochromatography, laser-induced fluorescence detection and electrospray-mass spectrometry.
Macroporous, monolithic capillary electrochromatography (CEC) columns, featuring a hydrophobic stationary phase, have been applied to the separations of steroids with good column efficiency. Using isocratic and gradient elution runs, mixtures of neutral or conjugated steroids could be resolved. While dansylated ketosteroids were detectable through laser-induced fluorescence at attomole levels, the CEC columns coupled to electrospray-ion-trap mass spectrometry featured femtomole detection limits.
Que
A H
AH
Department of Chemistry, Indiana University, Bloomington 47405, USA.
Palm
A
A
Baker
A G
AG
Novotny
M V
MV
D019075:Electrophoresis, Capillary / Q000379:methods*; D005260:Female; D006801:Humans; D007834:Lasers; D013058:Mass Spectrometry / Q000379:methods; D009994:Osmolar Concentration; D011247:Pregnancy; D012015:Reference Standards; D013050:Spectrometry, Fluorescence / Q000379:methods; D013256:Steroids / Q000032:analysis* / Q000652:urine
D013256:Steroids