A C. elegans mediator protein confers regulatory selectivity on lineage-specific expression of a transcription factor gene.
The Caenorhabditis elegans caudal homolog, pal-1, is required for neurogenesis in the male tail. We show that expression of pal-1 in the postembryonic neuroblast cell V6 can be initiated by two alternate pathways. One pathway, acting in wild type, requires a regulatory element in the fifth pal-1 intron. The other pathway, independent of this element, is normally repressed by the newly identified gene sop-1, which encodes a homolog of the mammalian Mediator complex protein TRAP230. In sop-1 mutants, pal-1 is activated by a pathway that is stimulated by bar-1/beta-catenin, a component of the Wnt signal transduction pathway. The results support a physiological role of the Mediator complex in conveying regulatory signals to the transcriptional apparatus.
Zhang
H
H
Department of Molecular Genetics, Albert Einstein College of Medicine, The Bronx, New York 10461, USA.
Emmons
S W
SW
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism*; D019070:Cell Lineage; D002467:Cell Nucleus / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D005838:Genotype; D015801:Helminth Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology*; D018398:Homeodomain Proteins; D007438:Introns; D008297:Male; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008954:Models, Biological; D008957:Models, Genetic; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010641:Phenotype; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D013489:Suppression, Genetic; D015534:Trans-Activators; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics*; D014158:Transcription, Genetic; D051153:Wnt Proteins; D029961:Zebrafish Proteins; D051176:beta Catenin
D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; D015801:Helminth Proteins; D018398:Homeodomain Proteins; D011518:Proto-Oncogene Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D051153:Wnt Proteins; D029961:Zebrafish Proteins; C115014:bar-1 protein, C elegans; D051176:beta Catenin; C413707:dpy-22 protein, C elegans; C068665:pal-1 protein, C elegans