Total tumor cell elimination with minimum damage to normal tissues in musculoskeletal sarcomas following photodynamic therapy with acridine orange.
Acridine orange (AO) has unique biological actions enabling tumor visualization (fluorovisualization) and a strong cytocidal effect (photodynamic therapy: AO-PDT) under illumination with blue light. Accordingly, in this study, we attempted to develop a new surgical technique for total tumor cell elimination using these photodynamic reactions with AO in a mouse osteosarcoma model. The results showed that local tumor recurrence was significantly inhibited (23%) in the group treated with curettage under fluorovisualization and AO-PDT, compared to that (80%) in the control group treated with curettage alone under ordinary light. Therefore, we concluded that the combination of curettage under fluorovisualization and AO-PDT may be useful for total tumor cell elimination with minimum damage to normal tissue in musculoskeletal sarcomas.
Kusuzaki
K
K
Orthopedic Surgery, Kyoto Prefectural University of Medicine, Kyoto, Japan. kusu@basic.kpu-m.ac.jp
Aomori
K
K
Suginoshita
T
T
Minami
G
G
Takeshita
H
H
Murata
H
H
Hashiguchi
S
S
Ashihara
T
T
Hirasawa
Y
Y
D000165:Acridine Orange / Q000627:therapeutic use*; D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D001859:Bone Neoplasms / Q000188:drug therapy*; D003475:Curettage; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009140:Musculoskeletal Diseases / Q000188:drug therapy; D009153:Mutagens / Q000627:therapeutic use; D012516:Osteosarcoma / Q000188:drug therapy*; D010778:Photochemotherapy; D016896:Treatment Outcome
D000970:Antineoplastic Agents; D009153:Mutagens; D000165:Acridine Orange