title: Specific cellular features of atheroma associated with development of neointima after carotid endarterectomy: the carotid atherosclerosis and restenosis study.
abstract: BACKGROUND
  The purpose of this study was to investigate whether some cellular and molecular features of tissue retrieved at carotid endarterectomy are associated with the extent of neointima formation at ultrasound follow-up.
  
  
  RESULTS
  One hundred fifty patients were studied. Endarterectomy specimens were tested by immunocytochemistry with the use of (1) monoclonal antibodies that identify smooth muscle cells (SMCs) and fetal-type SMCs on the basis of smooth muscle and nonmuscle myosin content, (2) the anti-macrophage HAM 56, and (3) the anti-lymphocyte CD45RO. The maximum intima-media thickness (M-IMT) of the revascularized vessel was assessed by the use of B-mode ultrasonography 6 months after surgery. The M-IMT values were related positively to the number of SMCs (r=0.534, P<0.0005) and negatively to that of macrophages and lymphocytes (r=-0.428, P<0.0005, and -0.538, P=0.001, respectively). Patients were classified as class 1 (M-IMT </=1.0 mm), class 2 (1. 0<M-IMT</=1.3 mm), and class 3 (M-IMT >1.3 mm). An abundance of SMCs, mostly of fetal type, was found in the plaque of class 3 patients, whereas lesions from class 1 patients were rich in macrophages and lymphocytes. In the multivariate analysis, factors related to M-IMT were the number of SMCs and the percentage of fetal-type SMCs present in the plaque.
  
  
  CONCLUSIONS
  Although the classic risk factors did not play a role, an abundance of SMCs and a scarcity of macrophages characterized the primary lesion of patients in whom neointima developed after surgery. In patients in whom neointima did not develop, lesions were rich in macrophages and lymphocytes. This approach can be useful in defining patients at risk of restenosis.
authors.affiliation: Dipartimento di Medicina Clinica e Sperimentale, Università degli Studi di Padova, Padova, Italy. pauletto@ux1.unipd.it
authors.forename: P
authors.initials: P
authors.lastname: Pauletto
authors.forename: M
authors.initials: M
authors.lastname: Puato
authors.forename: E
authors.initials: E
authors.lastname: Faggin
authors.forename: N
authors.initials: N
authors.lastname: Santipolo
authors.forename: V
authors.initials: V
authors.lastname: Pagliara
authors.forename: M
authors.initials: M
authors.lastname: Zoleo
authors.forename: G P
authors.initials: GP
authors.lastname: Deriu
authors.forename: F
authors.initials: F
authors.lastname: Grego
authors.forename: M
authors.initials: M
authors.lastname: Plebani
authors.forename: S
authors.initials: S
authors.lastname: Sartore
authors.forename: G B
authors.initials: GB
authors.lastname: Bon
authors.forename: C
authors.initials: C
authors.lastname: Heymes
authors.forename: J L
authors.initials: JL
authors.lastname: Samuel
authors.forename: A C
authors.initials: AC
authors.lastname: Pessina
mesh_terms: D000368:Aged; D002339:Carotid Arteries / Q000000981:diagnostic imaging / Q000473:pathology* / Q000601:surgery*; D002340:Carotid Artery Diseases / Q000000981:diagnostic imaging / Q000473:pathology* / Q000601:surgery*; D004691:Endarterectomy; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008214:Lymphocytes / Q000473:pathology; D008264:Macrophages / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009131:Muscle, Smooth, Vascular / Q000473:pathology; D011184:Postoperative Period; D012008:Recurrence; D012307:Risk Factors; D017539:Tunica Intima / Q000000981:diagnostic imaging / Q000254:growth & development / Q000473:pathology*; D017540:Tunica Media / Q000000981:diagnostic imaging; D014463:Ultrasonography