title: Inoculation of Sarcocystis neurona merozoites into the central nervous system of horses.
abstract: Equine protozoal myeloencephalitis (EPM) is a neurologic syndrome in horses from the Americas and is usually caused by infection with the apicomplexan parasite, Sarcocystis neurona. A horse model of EPM is needed to test the efficacy of chemotherapeutic agents and potential vaccines. Five horses that were negative for antibodies to S. neurona in their serum and cerebrospinal fluid (CSF) were injected in the subarachnoid space with living merozoites of the SN2 isolate of S. neurona. None of the horses developed clinical disease or died over a 132-day observation period. All five horses developed antibodies to S. neurona in their CSF and serum 3-4 weeks after injection. Two of the horses were examined at necropsy and no parasite induced lesions were observed in their tissues and no parasites were recovered from portions of their spinal cords inoculated on to cell cultures. Results of this study demonstrate that merozoites of the SN2 isolate of S. neurona will induce seroconversion but not clinical disease when inoculated directly into the CSF of nonimmune horses.
authors.affiliation: Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine and Infectious Diseases, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, 24061-0342, USA. lindsayd@vt.edu
authors.forename: D S
authors.initials: DS
authors.lastname: Lindsay
authors.forename: C C
authors.initials: CC
authors.lastname: Dykstra
authors.forename: A
authors.initials: A
authors.lastname: Williams
authors.forename: J A
authors.initials: JA
authors.lastname: Spencer
authors.forename: S D
authors.initials: SD
authors.lastname: Lenz
authors.forename: K
authors.initials: K
authors.lastname: Palma
authors.forename: J P
authors.initials: JP
authors.lastname: Dubey
authors.forename: B L
authors.initials: BL
authors.lastname: Blagburn
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood / Q000134:cerebrospinal fluid; D015153:Blotting, Western / Q000662:veterinary; D004679:Encephalomyelitis / Q000097:blood / Q000134:cerebrospinal fluid / Q000469:parasitology / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D018397:Equidae; D005260:Female; D006734:Horse Diseases / Q000469:parasitology*; D006736:Horses; D007278:Injections, Spinal / Q000662:veterinary; D008297:Male; D012522:Sarcocystis / Q000472:pathogenicity*; D012523:Sarcocystosis / Q000097:blood / Q000134:cerebrospinal fluid / Q000469:parasitology / Q000662:veterinary*
chemical_list: D000913:Antibodies, Protozoan