The inhibitory effect of anti-allergic agent suplatast tosilate (IPD-1151T) on methacholine- and allergen-induced bronchoconstriction in sensitized mice. asakazu@med.showa-u.dc.jp.
The influence of an anti-allergic agent, suplatast tosilate (IPD-1151T; (+/-)-[2-[4-(3-ethoxy-2-hydroxypropoxy)phenyl-carbamoyl]-ethyl] dimethylsulfonium p-toluenesulfonate) on allergic bronchoconstriction induced by allergen and methacholine (MCh) were examined in mice. BALB/c mice were sensitized by intraperitoneal injection of dinitrophenylated-keyhole limpet hemocyanin (DNP-KLH) mixed with A1(OH)3 (DNP-KLH). IPD-1151T was administered orally once a day for either 5 or 14 days in doses of 10, 30 or 100 mg/kg. Bronchoconstriction was measured 24h after the final drug administration. IPD-1151T inhibited both antigen- and MCh-mediated bronchoconstriction in actively sensitized mice. The inhibition induced was closely related to the dose and frequency of oral administration of the agent. We also examined the effect of IPD-1151T on IgE production in response to DNP-KLH immunization. IPD-1151T inhibited dose-dependently both total and specific IgE concentrations in serum prepared from mice 15 days after immunization. These results strongly indicate that IPD-1151T inhibits IgE production in vivo and results in attenuating effect on bronchoconstriction.
Asano
K
K
Department of Physiology, School of Medicine, Showa University, Tokyo, Japan. asakazu@med.showa-u.dc.jp
Mizutani
T
T
Shimane
T
T
Hisano
M
M
Hisamitsu
T
T
Suzaki
H
H
D000485:Allergens / Q000008:administration & dosage / Q000276:immunology*; D000818:Animals; D018926:Anti-Allergic Agents / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D000941:Antigens / Q000008:administration & dosage / Q000276:immunology*; D001190:Arylsulfonates / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D016084:Bronchoconstriction / Q000187:drug effects / Q000276:immunology*; D002478:Cells, Cultured; D006433:Hemocyanins / Q000008:administration & dosage / Q000276:immunology*; D006633:Histamine Antagonists / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D007073:Immunoglobulin E / Q000096:biosynthesis; D016210:Methacholine Chloride / Q000008:administration & dosage / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D015394:Molecular Structure; D013154:Spleen / Q000166:cytology; D013452:Sulfonium Compounds / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*
C042158:2,4-dinitrophenyl keyhole limpet hemocyanin; D000485:Allergens; D018926:Anti-Allergic Agents; D000941:Antigens; D001190:Arylsulfonates; D006633:Histamine Antagonists; D013452:Sulfonium Compounds; D016210:Methacholine Chloride; D007073:Immunoglobulin E; D006433:Hemocyanins; C073028:suplatast tosilate