Immunohistochemical analysis of BRCA2 expression in normal human buccal cells.
Previously we demonstrated that protein coded by the BRCA1 gene was expressed in normal human buccal cells. The present study confirmed that BRCA2 protein was similarly expressed in these cells. Messenger RNA for BRCA2 was detected with sequential use of two primer sets. Pooled cell samples from healthy donors reacted strongly with two commercially available antibodies, I17 and C15. Immunoreactivity was present in both cytoplasmic and nuclear compartments. We conclude buccal cells will provide a suitable model for exploration of normal BRCA function.
Byrne
T J
TJ
Department of Plant & Soil Sciences, University of Massachusetts, Amherst, MA, USA.
Cohn
G M
GM
Lane
M A
MA
Adams
L A
LA
Hoffman
D E
DE
Reece
M T
MT
D000328:Adult; D019313:BRCA1 Protein / Q000276:immunology / Q000378:metabolism; D024682:BRCA2 Protein; D002610:Cheek; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008297:Male; D009061:Mouth Mucosa / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology
D019313:BRCA1 Protein; D024682:BRCA2 Protein; D018076:DNA, Complementary; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D014157:Transcription Factors