Augmentative effect of polyethylene glycol, polyvinyl alcohol and dextran on thyroid-stimulating antibody stimulated cAMP production in FRTL-5 cells and CHO cells expressing human TSH receptor.
Previously we reported the augmentative effect of nonionic hydrophilic polymers such as polyethylene glycol (PEG), polyvinyl alcohol (PVA) and dextran on thyroid-stimulating antibody (TSAb) activity in porcine thyroid cell assays. We examined whether a similar phenomenon occurs in FRTL-5 thyroid cells and CHO cells expressing the human (h) TSH receptor (CHO-hTSHR cells). As with porcine thyroid cells, PEG 22.5% precipitated crude IgG from serum of patients with Graves' disease, significantly increased cAMP production as compared with PEG 12.5% precipitated crude IgG in both FRTL-5 cells and CHO-hTSHR cells. PEG 5% augmented purified-TSAb-IgG-stimulated cAMP production in both cell assays. TSAb activities and positivity by the direct assay using whole serum (0.05 ml) in the presence of 5% PEG in untreated Graves' patients were significantly increased as compared with the absence of 5% PEG. The augmentative effects of PVA 10% or dextran T-70 10% on TSAb-IgG-stimulated cAMP production were also observed in both cell assays. PVA 10% did not augment TSH-stimulated cAMP production in spite of weak augmentation by dextran 10% in both cell assays. Lack of the augmentative effects of PEG 5%, PVA 10% and dextran 10% on cAMP produced by GTPgammaS, forskolin and pituitary adenylate cyclase activating polypeptide was observed in both cell assays. The augmentative effects of these polymers in both cell assays similar to porcine thyroid cells suggest that there is no apparent species specificity among human, porcine and rat thyroid cells as far as TSH receptor linked cAMP production in cell membranes existed.
Ochi
Y
Y
Research Institute for Production Development, Kyoto, Japan.
Kajita
Y
Y
Wu
L
L
Iidaka
M
M
Nagayama
Y
Y
D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D011232:Chemical Precipitation; D006224:Cricetinae; D000242:Cyclic AMP / Q000096:biosynthesis*; D003911:Dextrans / Q000008:administration & dosage; D015870:Gene Expression; D006111:Graves Disease / Q000276:immunology; D006801:Humans; D018828:Immunoglobulins, Thyroid-Stimulating / Q000008:administration & dosage*; D011092:Polyethylene Glycols / Q000008:administration & dosage; D011108:Polymers / Q000008:administration & dosage*; D011142:Polyvinyl Alcohol / Q000008:administration & dosage; D051381:Rats; D011989:Receptors, Thyrotropin / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013552:Swine; D013961:Thyroid Gland / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D013972:Thyrotropin / Q000494:pharmacology
D003911:Dextrans; D018828:Immunoglobulins, Thyroid-Stimulating; D011108:Polymers; D011989:Receptors, Thyrotropin; D011994:Recombinant Proteins; D011092:Polyethylene Glycols; D013972:Thyrotropin; D011142:Polyvinyl Alcohol; D000242:Cyclic AMP