An improved rapid procedure for fluorescence in situ hybridization that is applicable to intraoperative cancer cytodiagnosis.
Fluorescence in situ hybridization (FISH) is among the most simple and useful methods for detecting numerical and structural aberration of chromosomes but it requires 12-24 h to complete. We devised a rapid FISH method that can be performed within 2 h. Here we describe the technique, which we have found to be extreme simple and as sensitive and specific as standard FISH, making it highly suitable for clinical use.
Koide
K
K
Department of Digestive Surgery, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Hirokohji, Kawaramachi-tori, Kamigyo-ku, Kyoto, Japan. koide@iwa.att.ne.jp
Sakakura
C
C
Hagiwara
A
A
Yamaguchi
T
T
Yamagishi
H
H
Abe
T
T
Inazawa
J
J
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D022981:Allelic Imbalance; D002869:Chromosome Aberrations; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics; D002887:Chromosomes, Human, Pair 18 / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics / Q000473:pathology; D003581:Cytodiagnosis; D003937:Diagnosis, Differential; D004938:Esophageal Neoplasms / Q000235:genetics / Q000473:pathology; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000235:genetics / Q000473:pathology*; D012680:Sensitivity and Specificity; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology; D013997:Time Factors; D020298:U937 Cells; D014998:Y Chromosome / Q000235:genetics