Interactions between liposomes and hydroxypropylmethylcellulose.
The characteristics of the adsorption process of hydroxypropylmethylcellulose (HPMC) of molecular weight 35400 Da and nominal viscosity 100 cps onto liposomes prepared with different egg lecithin-cholesterol molar ratios were examined. Adsorption isotherms were constructed and analysed to investigate the mechanisms implicated in the incorporation of the polymer to the interface. Only the isotherms obtained with cholesterol-free liposomes were fitted with Langmuir model. When cholesterol is present in the composition they present a sigmoidal slope. The mechanism of adsorption depends on liposome composition being the main force that drives polymer adsorption of hydrophobic nature. The apparent volumes of HPMC indicate that the conformation of the adsorbed macromolecules depends on liposome composition. Hydration enthalpy values show that adsorbed polymers do not give more hydrophilic systems after freeze-drying as expected with the hydrophilic characteristics of the HPMC.
Gutiérrez de Rubalcava
C
C
Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, Universidad de Santiago de Compostela, Campus Universitario Sur, 15706, Santiago de Compostela, Spain.
Rodriguez
J L
JL
Duro
R
R
Alvarez-Lorenzo
C
C
Concheiro
A
A
Seijo
B
B
D000327:Adsorption; D004337:Drug Carriers; D007785:Lactose / Q000008:administration & dosage / Q000031:analogs & derivatives*; D008081:Liposomes / Q000008:administration & dosage*; D008747:Methylcellulose / Q000008:administration & dosage / Q000031:analogs & derivatives*; D008968:Molecular Conformation; D010078:Oxazines; D012995:Solubility; D013816:Thermodynamics
D004337:Drug Carriers; D008081:Liposomes; D010078:Oxazines; D008747:Methylcellulose; C071191:MK 458; D007785:Lactose