Serial analysis of gene expression identifies putative metastasis-associated transcripts in colon tumour cell lines.
We have used serial analysis of gene expression (SAGE) to identify gene expression differences between a primary colon tumour cell line (SW480) and an isogenic lymph-node metastasis cell line (SW620). Differential expression was confirmed for the following genes: keratin K5, cystatin S, serum amyloid A, the human homologue of yeast ribosomal S28 and the p32 subunit of human pre-mRNA splicing factor SF2. Expression of confirmed differences were also analysed in other metastatic cell lines.
Parle-McDermott
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A
Department of Biochemistry, Royal College of Surgeons in Ireland, 123 St Stephen's Green, Dublin 2, Ireland.
McWilliam
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P
Tighe
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Dunican
D
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Croke
D T
DT
D015152:Blotting, Northern; D002454:Cell Differentiation; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured