11q23 allelic loss is associated with regional lymph node metastasis in melanoma.
Genetic alterations of the long arm of chromosome 11 have been implicated in melanoma pathogenesis, and we recently identified two distinct regions of common allelic loss in chromosomal band 11q23. To establish the point in time of melanoma tumorigenesis at which these two putative tumor suppressor loci become relevant, we investigated allelic loss [loss of heterozygosity (LOH)] in both chromosomal regions in tumors of progressing patients. We analyzed 102 tumor samples from 23 patients for whom at least two (10 patients) or three (13 patients) tumor samples from different clinical progression steps (such as primary tumor and/or in-transit metastasis and/or regional lymph node metastasis and/or distant metastasis) were available. We detected no 11q23 LOH at any stage in 3 of 23 patients and detected LOH at all stages tested in 8 of 23 patients. In 8 of the remaining 12 (67%) patients with 11q23 LOH at some stage during tumor progression, we found this to occur first at regional lymph node metastasis. Two of these patients retained constitutional heterozygosity in several in-transit metastases that developed up to 7 months after lymph node metastases that already had loss. We therefore conclude that 11q23 LOH is associated with regional lymph node metastasis in melanoma. Finally, we detected an allele shift restricted to a histomorphologically distinct part of a primary melanoma and found that the same parental chromosome was affected by allelic loss in a subsequently occurring lymph node metastasis. These findings support our conclusion and give additional evidence for the hypothesis of molecular heterogeneity of early tumor cell populations in melanoma.
Herbst
R A
RA
Department of Dermatology and Allergology, Hannover Medical University, Germany. herbst_ra@gmx.de
Mommert
S
S
Casper
U
U
Podewski
E K
EK
Kiehl
P
P
Kapp
A
A
Weiss
J
J
D000483:Alleles; D002455:Cell Division / Q000235:genetics; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics*; D018450:Disease Progression; D006801:Humans; D019656:Loss of Heterozygosity; D008207:Lymphatic Metastasis; D008545:Melanoma / Q000235:genetics* / Q000473:pathology / Q000556:secondary*