CREB-binding protein/p300 activates MyoD by acetylation.
The myogenic protein MyoD requires two nuclear histone acetyltransferases, CREB-binding protein (CBP)/p300 and PCAF, to transactivate muscle promoters. MyoD is acetylated by PCAF in vitro, which seems to increase its affinity for DNA. We here show that MyoD is constitutively acetylated in muscle cells. In vitro, MyoD is acetylated both by CBP/p300 and by PCAF on two lysines located at the boundary of the DNA binding domain. MyoD acetylation by CBP/p300 (as well as by PCAF) increases its activity on a muscle-specific promoter, as assessed by microinjection experiments. MyoD mutants that cannot be acetylated in vitro are not activated in the functional assay. Our results provide direct evidence that MyoD acetylation functionally activates the protein and show that both PCAF and CBP/p300 are candidate enzymes for MyoD acetylation in vivo.
Polesskaya
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A
Laboratoire Oncogenèse, Différenciation et Transduction du Signal, CNRS UPR 9079, Institut Fédératif André Lwoff, 7 rue Guy Moquet, Villejuif, France.
Duquet
A
A
Naguibneva
I
I
Weise
C
C
Vervisch
A
A
Bengal
E
E
Hucho
F
F
Robin
P
P
Harel-Bellan
A
A
D000107:Acetylation; D000123:Acetyltransferases / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D050881:E1A-Associated p300 Protein; D051548:Histone Acetyltransferases; D051379:Mice; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D017570:MyoD Protein / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators / Q000378:metabolism*; D015533:Transcriptional Activation
D017570:MyoD Protein; D009687:Nuclear Proteins; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D000123:Acetyltransferases; D050881:E1A-Associated p300 Protein; C494361:Ep300 protein, mouse; D051548:Histone Acetyltransferases