Chemiluminescence derivatization of methylglyoxal using 2-aminonicotinic acid.
To develop a sensitive and selective chemiluminometric method for the determination of methylglyoxal, we used 2-aminonicotinic acid as the chemiluminescence derivatization reagent. 2-Aminonicotinic acid reacts with methylglyoxal in an acidic solution at 37 degrees C for 4 h and gave a chemiluminescence in N,N'-dimethylformamide containing sodium tert-butoxide. The detection limit (blank intensity plus three-times its standard deviation of methylglyoxal) for methylglyoxal is 233 fmol in the reaction mixture.
Ohba
Y
Y
Graduate School of Pharmaceutical Sciences, Kyushu University, Higashi, Fukuoka, Japan.
Sakata
M
M
Zaitsu
K
K
D007700:Kinetics; D008163:Luminescent Measurements; D009539:Nicotinic Acids / Q000737:chemistry*; D011765:Pyruvaldehyde / Q000737:chemistry*; D013696:Temperature
D009539:Nicotinic Acids; C110415:2-aminonicotinic acid; D011765:Pyruvaldehyde