Clarithromycin targeting to lung: characterization, size distribution and in vivo evaluation of the human serum albumin microspheres.
Microspheres of clarithromycin have been prepared from human serum albumin using the emulsion polymerization technique. Albumin microspheres containing the active substance were injected into the tail vein of mice. Mice were sacrificed at intervals and microspheres collected from lungs and livers. The clarithromycin amount in microspheres was determined by reversed phase high performance liquid chromatographic (HPLC) method from the mice organs. Morphological and histopathological observations were also reported. The microsphere accumulation began at 10 min, and increased gradually until 6 h, then a decrease was observed. The microspheres were still present after 24 h. In the liver sample, no microsphere accumulation was observed at any time.
Ozkan
Y
Y
Gülhane Military Medical Academy, Department of Pharmaceutical Technology, Etlik-Ankara, Turkey. yozkan@obs.gata.edu.tr
Dikmen
N
N
Işimer
A
A
Günhan
O
O
Aboul-Enein
H Y
HY
D000418:Albumins / Q000378:metabolism*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000378:metabolism*; D017291:Clarithromycin / Q000008:administration & dosage / Q000378:metabolism*; D004337:Drug Carriers; D006801:Humans; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008863:Microspheres
D000418:Albumins; D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D017291:Clarithromycin