The Kluyver effect for trehalose in Saccharomyces cerevisiae.
Saccharomyces cerevisiae cells are able to grow using trehalose as a sole source of carbon and energy. However, the biomass yield obtained with trehalose was higher, and the specific growth rate lower, than that obtained with glucose or maltose. The respiratory inhibitor antimycin A prevented cell growth on trehalose, and no ethanol or glycerol was formed during batch growth on this carbon source. Thus, S. cerevisiae exhibits the KLUYVER effect for trehalose: this disaccharide is assimilated and respired, but, in contrast to glucose or maltose, it cannot be fermented. The high-affinity trehalose-H+ symporter encoded by the AGT1 gene is required for growth on trehalose. Analysis of the differences in the metabolism of maltose and trehalose (both disaccharides of glucose transported by active transport systems) indicated that the absence of trehalose fermentation is a consequence of low sugar influx into the cells during growth on this carbon source.
Malluta
E F
EF
Departamento de Bioquímica, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.
Decker
P
P
Stambuk
B U
BU
D000935:Antifungal Agents / Q000494:pharmacology; D000968:Antimycin A / Q000494:pharmacology; D018533:Biomass; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D003470:Culture Media; D000431:Ethanol / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D009004:Monosaccharide Transport Proteins; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000254:growth & development*; D029701:Saccharomyces cerevisiae Proteins; D027981:Symporters; D014198:Trehalase / Q000378:metabolism; D014199:Trehalose / Q000378:metabolism*
D000935:Antifungal Agents; D002352:Carrier Proteins; D003470:Culture Media; D005656:Fungal Proteins; D009004:Monosaccharide Transport Proteins; D029701:Saccharomyces cerevisiae Proteins; D027981:Symporters; C067575:maltose transport system, S cerevisiae; D000431:Ethanol; D000968:Antimycin A; D014199:Trehalose; D014198:Trehalase; D005990:Glycerol