title: Muscle fiber type composition and morphometric properties of denervated rat extensor digitorum longus muscle.
abstract: OBJECTIVE
  Analysis of fiber type composition and fiber size of extensor digitorum longus (EDL) muscle in rats during 30 days of denervation.
  
  
  METHODS
  Fiber types were defined immunohistochemically using monoclonal antibodies specific for slow (type I) and fast fibers (type IIA, IIX, and IIB). Antibodies were applied on transverse sections of denervated extensor digitorum longus muscles 6, 12, and 30 days after denervation. Cross sectional area of muscle fibers was analyzed morphometrically by computerized image analysis.
  
  
  RESULTS
  Control EDL muscle was composed of 41% of type IIB, 32% of IIA, 23% of IIX, and 4% of type I muscle fibers. The most profound effect of denervation was observed 30 days after the transection of the sciatic nerve. Denervation decreased the percentage of both type IIB and type IIX muscle fibers to 36% and 13% of the control muscle, respectively (p?0.001 for both), and increased the percentage of types IIA and type I muscle fibers to 42% and 9% of the control, respectively (p<0.001 for both). Morphometric analysis revealed progressive atrophy of all fast muscle fibers, which started 6 days after denervation (p<0.001). Thirty days after the sciatic nerve transection, a strong reduction in fiber size of type IIA, IIX, and IIB muscle fibers was observed (p<0.001 for each type). Type I muscle fibers initially showed the reduction in fiber size (p<0.001) but regained the size of control fibers until day 30.
  
  
  CONCLUSIONS
  Denervation decreased the percentage of type IIX and IIB muscle fibers, with concomitant increase in type IIA and type I muscle fibers. The reduction in fiber size was observed in type IIA, IIX, and IIB muscle fibers.
authors.affiliation: Department of Anatomy, Rijeka University School of Medicine, Brace Branchetta 22, 51000 Rijeka, Croatia. Dragica.Bobinac@mamed.medri.hr
authors.forename: D
authors.initials: D
authors.lastname: Bobinac
authors.forename: D
authors.initials: D
authors.lastname: Malnar-Dragojević
authors.forename: S
authors.initials: S
authors.lastname: Bajek
authors.forename: T
authors.initials: T
authors.lastname: Soić-Vranić
authors.forename: R
authors.initials: R
authors.lastname: Jerković
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D008297:Male; D009121:Muscle Denervation; D018485:Muscle Fibers, Skeletal / Q000145:classification* / Q000473:pathology; D018482:Muscle, Skeletal / Q000033:anatomy & histology*; D009133:Muscular Atrophy; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000911:Antibodies, Monoclonal