Enantioseparations in non-aqueous capillary electrochromatography using polysaccharide type chiral stationary phases.
Enantioseparations of chiral compounds with different structures were studied in non-aqueous capillary electrochromatography (NAQ CEC). Three different polysaccharide derivatives, cellulose tris(3,5-dimethylphenylcarbamate) (Chiralcel OD), amylose tris(3,5-dimethylphenylcarbamate) (Chiralpak AD) and cellulose tris(4-methylbenzoate) (Chiralcel OJ) were used as chiral stationary phases (CSPs). Methanolic or ethanolic ammonium acetate solutions served as a mobile phase. The effect of the type of the CSP, the loading of the chiral selector on wide-pore aminopropyl derivatized silica gel and operational parameters such as apparent pH, applied voltage, etc. on the EOF and chromatographic characteristics (alpha, N, Rs) were studied. NAQ CEC represents a valuable alternative and an extension to chiral separations by HPLC with common-size columns as well as to capillary LC and CEC in aqueous buffers.
Girod
M
M
Institute of Pharmaceutical Chemistry, University of Münster, Germany.
Chankvetadze
B
B
Blaschke
G
G
D000438:Alcohols / Q000737:chemistry; D002219:Carbamates / Q000737:chemistry; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D048448:Phenylcarbamates; D011134:Polysaccharides / Q000737:chemistry*; D058428:Silica Gel; D012822:Silicon Dioxide / Q000737:chemistry; D013237:Stereoisomerism
D000438:Alcohols; D002219:Carbamates; D048448:Phenylcarbamates; D011134:Polysaccharides; C065943:cellulose tris-3,5-dimethylphenyl-carbamate; D058428:Silica Gel; D012822:Silicon Dioxide; D002482:Cellulose