title: Prevention of pleuropneumonia in pigs by in-feed medication with sulphadimethoxine and sulphamethoxazole in combination with trimethoprim.
abstract: The prophylactic effect of in-feed medication of conventional pigs with sulphadimethoxine (SDM), sulphamethoxazole (SMX), and trimethoprim (TMP) was tested by using an Actinobacillus pleuropneumoniae infection model. In each of five experiments, six pigs were given medicated feed twice daily and three pigs received antibiotic-free feed and served as positive (unmedicated, infected) controls. The following drugs or drug combinations were tested (in mg per kg feed): 500 SDM + 100 TMP, 500 SMX + 100 TMP, 125 SMX + 25 TMP, 125 SMX (alone) and 25 TMP (alone). After six days of feed medication, all animals were endobronchially inoculated with A. pleuropneumoniae in a dose of 1-3.10(4) colony-forming units (CFU). The response to the challenge in all control pigs was characterized by fever, lethargy, anorexia, reduced water consumption, and laboured breathing. At autopsy all controls manifested a fibrinous haemorrhagic pleuropneumonia. In-feed medication with 500 SDM + 100 TMP, 500 SMX + 100 TMP as well as 125 SMX + 25 TMP resulted in an effective protection against the challenge in all treated animals. After consumption of feed medicated with 125 mg per kg SMX or 25 mg per kg TMP, pleuropneumonia was evident in all challenged pigs. The results of this study indicate an in vivo potentiation of SMX and TMP in pigs against this respiratory tract pathogen.
authors.affiliation: State Institute for Quality Control of Agricultural Products, Department of Toxicology, Wageningen, The Netherlands.
authors.forename: M J
authors.initials: MJ
authors.lastname: Mengelers
authors.forename: H A
authors.initials: HA
authors.lastname: Kuiper
authors.forename: A
authors.initials: A
authors.lastname: Pijpers
authors.forename: J H
authors.initials: JH
authors.lastname: Verheijden
authors.forename: A S
authors.initials: AS
authors.lastname: van Miert
mesh_terms: D000189:Actinobacillus Infections / Q000188:drug therapy / Q000517:prevention & control / Q000662:veterinary*; D016977:Actinobacillus pleuropneumoniae; D000821:Animal Feed; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000494:pharmacology / Q000627:therapeutic use; D001831:Body Temperature; D004195:Disease Models, Animal; D004338:Drug Combinations; D008297:Male; D011001:Pleuropneumonia / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D013412:Sulfadimethoxine / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D013420:Sulfamethoxazole / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D013552:Swine; D013553:Swine Diseases / Q000188:drug therapy / Q000382:microbiology / Q000517:prevention & control*; D014295:Trimethoprim / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D000900:Anti-Bacterial Agents; D004338:Drug Combinations; D013412:Sulfadimethoxine; D014295:Trimethoprim; D013420:Sulfamethoxazole