title: Mapping of leukaemia-associated breakpoints in chromosome band 3q21 using a newly established PAC contig.
abstract: Chromosome aberrations affecting band 3q21 are associated with a particularly poor prognosis in patients with acute myeloid leukaemia. To facilitate the molecular characterization of such rearrangements, we established a PAC contig covering the relevant genomic region. Using these PACs as probes in fluorescence in situ hybridization (FISH) experiments, we showed that a number of 3q21 breakpoints in patient samples map to a previously defined 'breakpoint cluster region'. Others, however, are located at varying distances centromeric of it. These results have important implications in the search for genes affected by 3q21 rearrangements.
authors.affiliation: Institut für Medizinische Biologie der Universität Wien, Austria. rotraud.wieser@univie.ac.at
authors.forename: R
authors.initials: R
authors.lastname: Wieser
authors.forename: A
authors.initials: A
authors.lastname: Volz
authors.forename: S
authors.initials: S
authors.lastname: Schnittger
authors.forename: U
authors.initials: U
authors.lastname: Jäger
authors.forename: H
authors.initials: H
authors.lastname: Grüner
authors.forename: J G
authors.initials: JG
authors.lastname: Meran
authors.forename: K
authors.initials: K
authors.lastname: Wimmer
authors.forename: A
authors.initials: A
authors.lastname: Ziegler
authors.forename: C
authors.initials: C
authors.lastname: Fonatsch
mesh_terms: D000208:Acute Disease; D000328:Adult; D000368:Aged; D019457:Chromosome Breakage / Q000235:genetics*; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics*; D020451:Contig Mapping / Q000379:methods*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007951:Leukemia, Myeloid / Q000235:genetics*; D008297:Male; D008875:Middle Aged