title: Cloning, crystallization and preliminary characterization of a beta-carbonic anhydrase from Escherichia coli.
abstract: Carbonic anhydrases are zinc metalloenzymes that fall into three distinct evolutionary and structural classes, alpha, beta and gamma. Although alpha-class enzymes, particularly mammalian carbonic anhydrase II, have been the subject of extensive structural studies, for the beta class, consisting of a wide variety of prokaryotic and plant chloroplast carbonic anhydrases, the structural data is quite limited. A member of the beta class from E. coli (CynT2) has been crystallized in native and selenomethionine-labelled forms and multiwavelength anomalous dispersion techniques have been applied in order to determine the positions of anomalous scatterers. The resulting phase information is sufficient to produce an interpretable electron-density map. A crystal structure for CynT2 would contribute significantly to the emerging structural knowledge of a biologically important class of enzymes that perform critical functions in carbon fixation and prokaryotic metabolism.
authors.affiliation: Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109-1024, USA.
authors.forename: J D
authors.initials: JD
authors.lastname: Cronk
authors.forename: J W
authors.initials: JW
authors.lastname: O'Neill
authors.forename: M R
authors.initials: MR
authors.lastname: Cronk
authors.forename: J A
authors.initials: JA
authors.lastname: Endrizzi
authors.forename: K Y
authors.initials: KY
authors.lastname: Zhang
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D002256:Carbonic Anhydrases / Q000737:chemistry* / Q000235:genetics; D002412:Cations; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D007527:Isoenzymes / Q000737:chemistry* / Q000235:genetics; D012643:Selenium / Q000737:chemistry; D012645:Selenomethionine / Q000737:chemistry; D015032:Zinc / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D002412:Cations; D007527:Isoenzymes; D012645:Selenomethionine; D002256:Carbonic Anhydrases; D012643:Selenium; D015032:Zinc