[Effects of infant formula feeding on the distribution of copper in the body of 8-day-old rats].
The oxidase activity of ceruloplasmin (Cp, EC 1.16.3.1), the content of immunoreactive Cp and copper ion concentration were measured in the serum of eight day-old rats receiving either breast feeding (control group) or commercial nutritive mixture which has been recommended for the newborn children beginning from zero age (experimental group). It was shown that the artificial feeding caused almost 3-fold increase of Cp oxidase activity and copper content in the serum when compared to age-matched controls. No changes in the copper content per Cp molecule were observed. Dot-hybridization of the total liver polyribosomal RNA with Cp [32P]cDNA showed that the increased Cp level in the blood of the rats of experimental group correlated well with the level of expression of Cp gene. The copper content in the liver of experimental rats was two times lower that in control animals while no differences was found in the brain copper content between two groups of rats. The role of the regulation of Cp gene expression in the lactating mammary gland and of milk Cp in the copper homeostasis in the newborn body is discussed.
Puchkova
L V
LV
Zhiguleva
E A
EA
Mokshina
S V
SV
Sasina
L K
LK
Tsymbalenko
N V
NV
Platonova
N A
NA
Giulikhandanova
N E
NE
Sviridova
T A
TA
Mishchenko
B S
BS
Gaĭtskhoki
V S
VS
D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000378:metabolism; D002570:Ceruloplasmin / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D003300:Copper / Q000097:blood / Q000378:metabolism*; D015870:Gene Expression; D007225:Infant Food; D008099:Liver / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D011132:Polyribosomes / Q000235:genetics; D012313:RNA / Q000032:analysis; D051381:Rats
D012313:RNA; D003300:Copper; D010088:Oxidoreductases; D002570:Ceruloplasmin