title: Argifin, a new chitinase inhibitor, produced by Gliocladium sp. FTD-0668. II. Isolation, physico-chemical properties, and structure elucidation.
abstract: A new chitinase inhibitor, named argifin, was isolated from the cultured broth of a fungal strain Gliocladium sp. FTD-0668. Argifin was purified from the cultured mycelium by the combination of cation exchange, anion exchange, adsorption, and gel filtration chromatographic methods. The structure of argifin was elucidated as cyclo(N(omega)-(N-methylcarbamoyl)-L-arginyl-N-methyl-L-phenylalan yl-beta-L-aspartyl-beta-L-aspartyl-D-alanyl) by NMR experiments and other spectroscopic analyses.
authors.affiliation: Research Center for Biological Function, The Kitasato Institute, Tokyo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Arai
authors.forename: K
authors.initials: K
authors.lastname: Shiomi
authors.forename: Y
authors.initials: Y
authors.lastname: Iwai
authors.forename: S
authors.initials: S
authors.lastname: Omura
mesh_terms: D002688:Chitinases / Q000037:antagonists & inhibitors*; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010456:Peptides, Cyclic / Q000737:chemistry* / Q000302:isolation & purification*; D016339:Spectrometry, Mass, Fast Atom Bombardment
chemical_list: D004791:Enzyme Inhibitors; D010456:Peptides, Cyclic; C413566:argifin; D002688:Chitinases