Purification of rabbit lacrimal gland plasma membranes by aqueous two-phase affinity partitioning.
We describe the purification of lacrimal gland plasma membranes by affinity partitioning using a two-phase system containing polyethylene glycol and dextran in which wheat germ agglutinin conjugated to dextran is used as affinity ligand. When partitioning a microsomal fraction, the plasma membrane marker 5'-nucleotidase was obtained in the affinity ligand-containing bottom phase, whereas the endoplasmic reticulum marker NADH-ferricyanide reductase remained in the top phase. The affinity partitioning behaviour of components involved in exocytosis and cellular signalling was also examined.
Ekblad
L
L
Department of Biochemistry, Center for Chemistry and Chemical Engineering, Lund University, Sweden.
Jergil
B
B
Gierow
J P
JP
D019870:1-Phosphatidylinositol 4-Kinase / Q000378:metabolism; D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D002846:Chromatography, Affinity / Q000379:methods*; D004721:Endoplasmic Reticulum / Q000201:enzymology; D005089:Exocytosis; D005260:Female; D007425:Intracellular Membranes; D007765:Lacrimal Apparatus / Q000648:ultrastructure*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D011817:Rabbits; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D009247:NADH, NADPH Oxidoreductases; C014227:ferricyanide reductase; D019870:1-Phosphatidylinositol 4-Kinase; D000254:Sodium-Potassium-Exchanging ATPase