title: Comparison of a ligase chain reaction-based assay and cell culture for detection of pharyngeal carriage of Chlamydia trachomatis.
abstract: In 264 genitourinary medicine clinic attenders reporting recent fellatio, the prevalence of pharyngeal Chlamydia trachomatis determined by an expanded standard including cell culture and two in-house PCR tests was 1.5% in 194 women and zero in 70 men. The ligase chain reaction (Abbott LCx) had a specificity of 99.2% and a positive predictive value of 60%.
authors.affiliation: Department of Genitourinary Medicine, Whittall St. Clinic, Birmingham, United Kingdom. Andy_Winter@talk21.com
authors.forename: A J
authors.initials: AJ
authors.lastname: Winter
authors.forename: G
authors.initials: G
authors.lastname: Gilleran
authors.forename: K
authors.initials: K
authors.lastname: Eastick
authors.forename: J D
authors.initials: JD
authors.lastname: Ross
mesh_terms: D000328:Adult; D001431:Bacteriological Techniques; D002353:Carrier State / Q000175:diagnosis* / Q000382:microbiology; D002690:Chlamydia Infections / Q000175:diagnosis* / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D003470:Culture Media; D005260:Female; D006801:Humans; D022521:Ligase Chain Reaction; D008297:Male; D010614:Pharynx / Q000382:microbiology*
chemical_list: D003470:Culture Media