Temporal and spatial expression of the cell-cycle regulator cul-1 in Drosophila and its stimulation by radiation-induced apoptosis.
Cul-1 protein is part of the ubiquitin ligase complex that is conserved from yeast to humans. This complex specifically marks cell-cycle regulators for their subsequent destruction. Two null mutations of the cul-1 gene are known, in budding yeast and in nematodes. Although in both these organisms the cul-1 gene executes essentially the same function, the manifestation of its lack-of-function mutations differs considerably. In yeast the mutation causes arrest at the G(1)/S-phase transition, whereas in nematodes excessive cell divisions occur because mutant cells are unable to exit the mitotic cycle. We isolated cul-1 orthologues from two model organisms, Drosophila melanogaster and mouse. We show that the Drosophila full-length cul-1 gene restores the yeast mutant's inability to pass through the G(1)/S-phase transition. We also characterize expression of this gene at the transcript and protein levels during Drosophila development and show that cul-1 gene is maternally supplied as a protein, but not as an RNA transcript. Zygotic transcription of the gene, however, resumes at early stages of embryogenesis. We also found an increase in cul-1 transcription in cultured cells treated with a lethal dose of gamma-irradiation.
Filippov
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V
Department of Cell Biology and Neuroscience Graduate Programs in Biochemistry and Molecular Biology, University of California, Riverside, CA 92521, USA.
Filippova
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Sehnal
F
F
Gill
S S
SS
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D001483:Base Sequence; D002453:Cell Cycle / Q000235:genetics; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D044842:Cullin Proteins; D017931:DNA Primers / Q000235:genetics; D004331:Drosophila melanogaster / Q000166:cytology* / Q000235:genetics* / Q000528:radiation effects; D005260:Female; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D005816:Genetic Complementation Test; D006801:Humans; D017403:In Situ Hybridization; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D009866:Oogenesis / Q000235:genetics; D010802:Phylogeny; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid
C100988:Cdc53 protein, S cerevisiae; D018797:Cell Cycle Proteins; C100318:Cullin 1; D044842:Cullin Proteins; D017931:DNA Primers; D019476:Insect Proteins; D029701:Saccharomyces cerevisiae Proteins