Isolation of a new laccase isoform from the white-rot fungi Pycnoporus cinnabarinus strain ss3.
Two extracellular laccase isoforms (Lac I and Lac II) produced by the white-rot fungus Pycnoporus cinnabarinus from the monokaryotic strain ss3 were purified from ferulic-acid-induced liquid culture medium using ammonium sulphate precipitation, followed by anion-exchange chromatography on a Mono Q column. Strain ss3 is the first generation of the parental strain P. cinnabarinus I-937. The new isolated isoform, Lac II, consists of an 86,000 molecular weight protein as determined by SDS polyacrylamide gel electrophoresis. The N-terminal amino acid sequences of both isoforms were determined, and compared to known laccase protein sequences of other organisms.
Otterbein
L
L
Laboratoire de Biotechnologie des Champignons Filamenteux, INRA, CESB-ESIL, Faculté des Sciences de Luminy, Parc Scientifique et Technologique, Marseille, France. otterbein@esil.univ-mrs.fr
Record
E
E
Chereau
D
D
Herpoël
I
I
Asther
M
M
Moukha
S M
SM
D000595:Amino Acid Sequence; D001487:Basidiomycota / Q000201:enzymology* / Q000254:growth & development; D003470:Culture Media; D007527:Isoenzymes / Q000737:chemistry / Q000302:isolation & purification; D042845:Laccase; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000737:chemistry / Q000302:isolation & purification*
D003470:Culture Media; D007527:Isoenzymes; D010088:Oxidoreductases; D042845:Laccase