title: Optimized procedures for analyzing primary alkylamines in wines by pentafluorobenzaldehyde derivatization and GC-MS.
abstract: Biogenic primary alkylamines in wines are toxicologically significant and affect sensory properties. An optimized method for analysis in wines involving derivatization with pentafluorobenzaldehyde (PFB) to corresponding pentafluorobenzylimines, liquid-liquid extraction, and gas chromatography with mass selective detection is presented. Reaction parameters including pH, temperature, time, and derivatizing agent and amine concentration were varied in simulated wine solution (15% ethanol) to determine effect on reaction efficiency. Optimal reaction efficiency was characterized (pH 12, 24 degrees C, 30 min, and 10 mg/mL PFB), and parameters were used for the analysis of 10 biogenic alkylamines in 12 California wines. Alkylamine concentration in wines ranged from 0.048 to 91 mg/L. Amine recoveries from wines at five fortification levels (0.1-85 mg/L) were generally 81-100%.
authors.affiliation: Department of Environmental Toxicology and Department of Viticulture and Enology, University of California, Davis 95616, USA.
authors.forename: K K
authors.initials: KK
authors.lastname: Ngim
authors.forename: S E
authors.initials: SE
authors.lastname: Ebeler
authors.forename: M E
authors.initials: ME
authors.lastname: Lew
authors.forename: D G
authors.initials: DG
authors.lastname: Crosby
authors.forename: J W
authors.initials: JW
authors.lastname: Wong
mesh_terms: D000588:Amines / Q000032:analysis* / Q000737:chemistry; D001547:Benzaldehydes / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007202:Indicators and Reagents / Q000737:chemistry*; D014920:Wine / Q000032:analysis*; D005464:Fluorobenzenes; D005466:Fluorocarbons
chemical_list: D000588:Amines; D001547:Benzaldehydes; D007202:Indicators and Reagents; C413389:pentafluorobenzaldehyde; D005464:Fluorobenzenes; D005466:Fluorocarbons