Role of reconstituted basement membrane in human granulosa cell culture.
The effects of Matrigel, a reconstituted basement membrane, on human granulosa cells were investigated. Cells were obtained from follicular aspirate in the course of oocyte retrieval for in vitro fertilization and were cultured on either a surface coated with Matrigel or uncoated plastic. Light and electron microscopy showed that granulosa cells cultured on Matrigel demonstrated three-dimensional aggregated cells with well differentiated morphology: numerous lipid droplets, microvilli, junctional complexes and lumen-like structures were seen. In contrast, cells cultured on plastic were flattened, poorly differentiated and showed apoptotic cells. Immunocytochemistry showed that the proportion of immunopositive cells for 3beta-hydroxysteroid dehydrogenase was increased in cultures on Matrigel. The results of the present study suggest that culture on Matrigel promotes the differentiation of human granulosa cells and provides a useful tool which may improve the efficiency of in vitro fertilization.
Hwang
D H
DH
Department of Anatomy, College of Medicine, Seoul National University, Jongro-Gu, Korea.
Kee
S H
SH
Kim
K
K
Cheong
K S
KS
Yoo
Y B
YB
Lee
B L
BL
D015096:3-Hydroxysteroid Dehydrogenases / Q000032:analysis; D017209:Apoptosis; D002448:Cell Adhesion; D002454:Cell Differentiation; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology*; D003470:Culture Media; D004338:Drug Combinations; D005260:Female; D006107:Granulosa Cells / Q000201:enzymology / Q000648:ultrastructure*; D006801:Humans; D007150:Immunohistochemistry; D007365:Intercellular Junctions / Q000648:ultrastructure; D007797:Laminin / Q000494:pharmacology*; D008055:Lipids / Q000032:analysis; D008854:Microscopy, Electron; D008871:Microvilli / Q000648:ultrastructure; D011509:Proteoglycans / Q000494:pharmacology*
D003470:Culture Media; D004338:Drug Combinations; D007797:Laminin; D008055:Lipids; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen; D015096:3-Hydroxysteroid Dehydrogenases