title: Molecular cloning and characterization of a metal responsive Aedes aegypti intestinal mucin cDNA.
abstract: We have isolated a cDNA from Aedes aegypti that is transcribed in the larval midgut in response to metal exposure, and in the adult female midgut in response to iron or cadmium exposure, or a blood meal. The cDNA encodes a protein, designated Aedes aegypti intestinal mucin 1 (AEIMUC1), which has similarities with invertebrate intestinal mucins and peritrophins, and vertebrate mucins. Proline, serine and threonine comprise 30% of the amino acid composition of AEIMUC1, a characteristic of mucins. AEIMUC1 contains three cysteine-rich domains, two of which flank a proline/serine/threonine-rich domain, a feature shared by many mucin genes. This is the first report on the isolation of a metal-responsive gene from an aquatic insect.
authors.affiliation: Arthropod-borne & Infectious Disease Laboratory, Center for Environmental Toxicology & Technology, Department of Microbiology, Colorado State University, Fort Collins, Colorado, USA. arayms@lamar.colostate.edu
authors.forename: A
authors.initials: A
authors.lastname: Rayms-Keller
authors.forename: M
authors.initials: M
authors.lastname: McGaw
authors.forename: C
authors.initials: C
authors.lastname: Oray
authors.forename: J O
authors.initials: JO
authors.lastname: Carlson
authors.forename: B J
authors.initials: BJ
authors.lastname: Beaty
mesh_terms: D000330:Aedes / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004064:Digestive System; D005260:Female; D006801:Humans; D008670:Metals; D008969:Molecular Sequence Data; D009077:Mucins / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D008670:Metals; D009077:Mucins