Genomic structure and in vivo expression of the human organic anion transporter 1 (hOAT1) gene.
The human organic anion transporter 1 (hOAT1) plays a key role in the secretion of an array of potentially toxic organic anions including many clinically important drugs. Here we report on the genomic cloning of hOAT1. A human genomic library was used for screening of a PAC (P1 artificial chromosome) clone applying PCR techniques. Sequencing of several restriction subclones and of a PCR-generated clone revealed that the hOAT1 gene spans 8.2 kb and is composed of 10 exons divided by 9 introns. RT-PCR studies in a human kidney specimen led to the detection of two new splice variants, hOAT1-3 and hOAT1-4, showing a 132-bp in-frame deletion. Using fluorescence in situ hybridization (FISH) we mapped the hOAT1 gene as a single signal to chromosome 11q13.1-q13.2. Additionally, 600 bp of the 5' flanking region was analyzed, illustrating the probable transcription start site at nt -280, a NF-kappaB-site at nt -397 and several putative transcription factor binding sites.
Bahn
A
A
Zentrum für Physiologie und Pathophysiologie, Humboldtallee 23, Göttingen, 37073, Germany. abahn@veg-physiol.med.uni-goettingen.de
Prawitt
D
D
Buttler
D
D
Reid
G
G
Enklaar
T
T
Wolff
N A
NA
Ebbinghaus
C
C
Hillemann
A
A
Schulten
H J
HJ
Gunawan
B
B
Füzesi
L
L
Zabel
B
B
Burckhardt
G
G
D027321:Anion Transport Proteins; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D004247:DNA; D017931:DNA Primers; D005091:Exons; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007438:Introns; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction
D027321:Anion Transport Proteins; D002352:Carrier Proteins; D017931:DNA Primers; D004247:DNA