Paramecium GPI proteins: variability of expression and localization.
In Paramecium primaurelia, the two major classes of cell surface proteins, the surface antigen (SAg) and the surface GPI proteins (SGPs), are linked to the plasma membrane through a glycosylphosphatidylinositol (GPI) anchor. In the present study, we have characterized the expression of the SGPs in several geographical strains of P. primaurelia and P. tetraurelia at different temperatures, 23 degrees C and 32 degrees C. The identification of the expressed SGPs was performed on purified cilia, by establishing the SGP SDS-PAGE profiles under four different conditions: with or without their anchoring lipid, cleaved with a Bacillus thuringiensis phosphatidylinositol-specific phospholipase C (PI-PLC), and either in a reduced or in an unreduced state. This screening revealed the existence of specific sets of ciliary SGPs, as a function of temperature and the geographical origin of the strains. The SGPs the most abundant at 23 degrees C and 32 degrees C displayed a rapid turnover. We also looked for the presence of PI-PLC releasable proteins in purified cortices. In addition to the SAg and SGPs, the cortical fraction was shown to contain other PI-PLC releasable proteins, not found in the ciliary fraction, thus localized exclusively in the interciliary region.
Capdeville
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Centre de Génétique Moléculaire, Centre National de la Recherche Scientifique, associé à l'Université Pierre et Marie Curie, Gif-sur-Yvette, France. capdeville@cgm.cnrs-gif.fr
D000818:Animals; D000953:Antigens, Protozoan / Q000096:biosynthesis / Q000302:isolation & purification; D000954:Antigens, Surface / Q000096:biosynthesis / Q000302:isolation & purification; D005591:Chemical Fractionation; D017261:Glycosylphosphatidylinositols / Q000378:metabolism*; D008565:Membrane Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D010247:Paramecium / Q000254:growth & development / Q000378:metabolism*; D015800:Protozoan Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D013696:Temperature
D000953:Antigens, Protozoan; D000954:Antigens, Surface; D017261:Glycosylphosphatidylinositols; D008565:Membrane Proteins; D015800:Protozoan Proteins