Comparison of different immunoaffinity clean-up procedures for high-performance liquid chromatographic analysis of ochratoxin A in wines.
Three immunoaffinity clean-up procedures to analyse ochratoxin A (OTA) in wines were compared. The direct wine clean-up with Ochraprep and OchraTest columns gave equivalent results in terms of recovery and precision if compared with the reference procedure involving a preliminary extraction of OTA with chloroform. OTA quantification limit in wine ranged from 0.020 to 0.045 microg/l. The 'on-flow' OTA emission spectrum (excitation 333 nm) showed a maximum at 460 nm and could be used to confirm the quantitative results. The analysis of 11 red and white wines gave no significant quantitative differences between the three clean-up techniques.
Castellari
M
M
Università degli Studi di Bologna, Corso di Laurea in Scienze e Tecnologie Alimentari, Cesena (FC), Italy. cast@foodsci.unibo.it
Fabbri
S
S
Fabiani
A
A
Amati
A
A
Galassi
S
S
D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009183:Mycotoxins / Q000032:analysis*; D009793:Ochratoxins / Q000032:analysis*; D012680:Sensitivity and Specificity; D014920:Wine / Q000032:analysis*
D009183:Mycotoxins; D009793:Ochratoxins; C025589:ochratoxin A