Increased immunoreactivity of glutathione-S-transferase in the retina of Swiss Webster mice following inhalation of JP8 + 100 aerosol.
The current study was designed to determine whether exposure of mice to aerosolized jet fuel (JP8 + 100) resulted in changes in the cellular distribution or immunoreactivity of the enzyme glutathione S-transferase (GST), a biomarker of toxicant exposure. Male mice were exposed to JP8 + 100 at 1000 mg/m3 or 2500 mg/m3 in aerosol for 1 h per day for 7 days and then sacrificed. The retinas were studied by immunohistochemical methods. The JP8 + 100 exposure caused a marked increase in the immunoreactivity of anti-GSTM antibodies with the radial glial cells of the retina, the Müller cells. These results are consistent with the hypothesis that JP8 + 100 acts as a toxicant to mouse retina by permitting the flux of materials across the blood-retina barrier. The findings are relevant to humans because recent studies indicate that Air Force personnel assigned to clean and maintain fuel pods may be exposed to concentrations of JP8 + 100 exceeding 1000 mg/m3.
McGuire
S
S
The Waisman Center, University of Wisconsin-Madison, 53705, USA.
Bostad
E
E
Smith
L
L
Witten
M
M
Siegel
F L
FL
Kornguth
S
S
D000336:Aerosols; D000818:Animals; D000906:Antibodies; D015415:Biomarkers / Q000032:analysis; D005982:Glutathione Transferase / Q000276:immunology*; D006838:Hydrocarbons / Q000633:toxicity*; D007150:Immunohistochemistry; D019570:Inhalation Exposure; D008297:Male; D051379:Mice; D009457:Neuroglia / Q000187:drug effects; D012160:Retina / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology*
D000336:Aerosols; D000906:Antibodies; D015415:Biomarkers; D005982:Glutathione Transferase; D006838:Hydrocarbons; C098607:JP8 aviation fuel