title: A high-throughput screening utilizing intramolecular fluorescence resonance energy transfer for the discovery of the molecules that bind HIV-1 TAR RNA specifically.
abstract: A 16-residue peptide, including the Tat(49-57) sequence was labeled with a fluorescein and a tetramethylrhodamine at its N- and C-terminus, respectively. This double dye-labeled peptide was prepared as a tracer for high-throughput screening utilizing intramolecular fluorescence resonance energy transfer (FRET). The binding of the competitor molecules for HIV-1 TAR RNA were monitored and dissociation constants of those molecule were determined by using this tracer. This novel screening system might be useful to discover the drug for HIV-1 TAR RNA.
authors.affiliation: Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Yokohama, Japan.
authors.forename: C
authors.initials: C
authors.lastname: Matsumoto
authors.forename: K
authors.initials: K
authors.lastname: Hamasaki
authors.forename: H
authors.initials: H
authors.lastname: Mihara
authors.forename: A
authors.initials: A
authors.lastname: Ueno
mesh_terms: D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005452:Fluoresceins / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D015696:Gene Products, tat / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016325:HIV Long Terminal Repeat / Q000502:physiology*; D006801:Humans; D015394:Molecular Structure; D010446:Peptide Fragments / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D010455:Peptides / Q000138:chemical synthesis* / Q000378:metabolism; D013050:Spectrometry, Fluorescence
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; D015696:Gene Products, tat; D010446:Peptide Fragments; D010455:Peptides; C418105:fluorescent Tat protein (49-57)