FTIR spectroscopic and HPLC chromatographic studies of carbon tetrachloride induced acute hepatitis in rats: damage in liver phospholipid membrane.
Carbon tetrachloride (CCl4) induced rat hepatitis was studied by observing an FTIR spectrum of the liver microsomal or homogenate extract compared with those of model compounds. The microsomal extract from the liver of healthy control rats showed almost the same spectrum as a mixture of phosphatidylcholine and phosphatidylethanolamine (2:1 by weight). Intraperitoneal injection of CCl4 decreased the absorption intensity due to th --C--H in the--C==H at 3012 cm(-1) in the microsomal extract, and it developed a new 1,2-diacylglycerol band at 1070 cm(-1) in the homogenate extract. An HPLC study was added to assign the 1070 cm(-1) band to 1,2-diacylglycerol. These findings were interpreted from the peroxidation of the microsomal membrane and the regenerative proliferation of the damaged cell.
Yoon
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S
Department of Veterinary Environmental Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Kazusaka
A
A
Fujita
S
S
D000208:Acute Disease; D000818:Animals; D002251:Carbon Tetrachloride; D002462:Cell Membrane / Q000737:chemistry / Q000473:pathology; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004075:Diglycerides / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008862:Microsomes, Liver / Q000737:chemistry; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017550:Spectroscopy, Fourier Transform Infrared
D004075:Diglycerides; D010743:Phospholipids; D002251:Carbon Tetrachloride