title: Analysis of genetic heterogeneity in Chlamydia trachomatis clinical isolates of serovars D, E, and F by amplified fragment length polymorphism.
abstract: Amplified fragment length polymorphism (AFLP) fingerprinting of clinical isolates of Chlamydia trachomatis serovars D, E, and F showed a low percentage of genetic heterogeneity, but clear differences were found. Isolates from index patients and partners had identical AFLP patterns and AFLP markers. Characterization of these AFLP markers could give more insight into the differences in virulence and clinical course of C. trachomatis infections.
authors.affiliation: Department of Pathology, Section of Molecular Pathology, University Hospital Vrije Universiteit, Amsterdam, The Netherlands.
authors.forename: S A
authors.initials: SA
authors.lastname: Morré
authors.forename: J M
authors.initials: JM
authors.lastname: Ossewaarde
authors.forename: P H
authors.initials: PH
authors.lastname: Savelkoul
authors.forename: J
authors.initials: J
authors.lastname: Stoof
authors.forename: C J
authors.initials: CJ
authors.lastname: Meijer
authors.forename: A J
authors.initials: AJ
authors.lastname: van den Brule
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D002690:Chlamydia Infections / Q000382:microbiology*; D002692:Chlamydia trachomatis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016172:DNA Fingerprinting / Q000379:methods*; D005260:Female; D052776:Female Urogenital Diseases / Q000382:microbiology; D014644:Genetic Variation; D006801:Humans; D008297:Male; D052801:Male Urogenital Diseases; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D018272:Porins; D012703:Serotyping; D014566:Urogenital System / Q000382:microbiology*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D018272:Porins; C071677:omp1 protein, Chlamydia trachomatis