title: Escherichia coli K1 aslA contributes to invasion of brain microvascular endothelial cells in vitro and in vivo.
abstract: Neonatal Escherichia coli meningitis remains a devastating disease, with unacceptably high morbidity and mortality despite advances in supportive care measures and bactericidal antibiotics. To further our ability to improve the outcome of affected neonates, a better understanding of the pathogenesis of the disease is necessary. To identify potential bacterial genes which contribute to E. coli invasion of the blood-brain barrier, a cerebrospinal fluid isolate of E. coli K1 was mutagenized with TnphoA. TnphoA mutant 27A-6 was found to have a significantly decreased ability to invade brain microvascular endothelial cells compared to the wild type. In vivo, 32% of the animals infected with mutant 27A-6 developed meningitis, compared to 82% of those infected with the parent strain, despite similar levels of bacteremia. The DNA flanking the TnphoA insertion in 27A-6 was cloned and sequenced and determined to be homologous to E. coli K-12 aslA (arylsulfatase-like gene). The deduced amino acid sequence of the E. coli K1 aslA gene product shows homology to a well-characterized arylsulfatase family of enzymes found in eukaryotes, as well as prokaryotes. Two additional aslA mutants were constructed by targeted gene disruption and internal gene deletion. Both of these mutants demonstrated decreased invasion phenotypes, similar to that of TnphoA mutant 27A-6. Complementation of the decreased-invasion phenotypes of these mutants was achieved when aslA was supplied in trans. This is the first demonstration that this locus contributes to invasion of the blood-brain barrier by E. coli K1.
authors.affiliation: Division of Infectious Diseases, Childrens Hospital Los Angeles, Los Angeles, California 90027, USA. jhoffman@chla.usc.edu
authors.forename: J A
authors.initials: JA
authors.lastname: Hoffman
authors.forename: J L
authors.initials: JL
authors.lastname: Badger
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: S H
authors.initials: SH
authors.lastname: Huang
authors.forename: K S
authors.initials: KS
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D001192:Arylsulfatases / Q000235:genetics* / Q000502:physiology; D001483:Base Sequence; D001921:Brain / Q000098:blood supply / Q000382:microbiology*; D002478:Cells, Cultured; D002874:Chromosome Mapping; D004251:DNA Transposable Elements; D004730:Endothelium, Vascular / Q000166:cytology / Q000382:microbiology*; D004926:Escherichia coli / Q000235:genetics* / Q000472:pathogenicity; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D006801:Humans; D007223:Infant; D008969:Molecular Sequence Data; D014774:Virulence
chemical_list: D004251:DNA Transposable Elements; D001192:Arylsulfatases