Genetic analysis of an incomplete mutS gene from Pseudomonas putida.
We genetically characterized the Pseudomonas putida mutS gene and found that it encodes a smaller MutS protein than do the genes of other bacteria. This gene is able to function in the mutS mutants of Escherichia coli and Bacillus subtilis. A P. putida mutS mutant has a mutation frequency 1,000-fold greater than that of the wild-type strain.
Kurusu
Y
Y
Laboratory of Molecular Microbiology, School of Agriculture, Ibaraki University, Ami, Japan. krsy@ipc.ibaraki.ac.jp
Narita
T
T
Suzuki
M
M
Watanabe
T
T
D000251:Adenosine Triphosphatases; D001412:Bacillus subtilis / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D004260:DNA Repair / Q000235:genetics; D004268:DNA-Binding Proteins; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D008969:Molecular Sequence Data; D051719:MutS DNA Mismatch-Binding Protein; D009154:Mutation; D016133:Polymerase Chain Reaction; D016958:Pseudomonas putida / Q000235:genetics*; D017384:Sequence Deletion
D001426:Bacterial Proteins; D017931:DNA Primers; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D000251:Adenosine Triphosphatases; D051719:MutS DNA Mismatch-Binding Protein; C497175:MutS protein, E coli