Determinants of umbilical cord arterial 8-iso-prostaglandin F2alpha concentrations.
OBJECTIVE
To determine the concentration of 8-isoPGF(2alpha) in cord blood as a measure of oxidative stress during labour, and to compare them with other established parameters of in vivo lipid peroxidation and with the acid-base status of the newborn.


METHODS
Umbilical cord arterial and venous blood samples were collected from 81 singleton term deliveries for determination of 8-isoPGF(2alpha), malondialdehyde and organic hydroperoxides. In addition, metabolites derived from the oxidative metabolism of purines during hypoxia-reoxygenation and routine cord blood of oxygen saturation, pH, pO2, pCO2, HCO3 and base excess were measured.


RESULTS
Arterial concentrations of 8-isoPGF(2alpha) were significantly higher in cases with fetal distress, tight nuchal cord (P < 0.001), the umbilical coiling index, and male sex (P < 0.05) (R2 = 0.48). No correlation was found with any parameter of acid-base status. In arterial and venous blood the concentrations of organic hydroperoxides and hypoxanthine significantly correlated with the fetal nuchal cord (P < 0.001) (R2 = 0.26 and 0.16, respectively).


CONCLUSIONS
Our findings indicate that 8-isoPGF2(alpha) in cord arterial blood is a suitable parameter to quantify a possible oxidative stress in the fetus during labour. Measurements of the F2-isoprostane concentrations in cord blood at labour provide a clinically useful method to assess the perinatal outcome.
Qin
Y
Y
Department of Obstetrics and Gynaecology, Prince of Wales Hospital, The Chinese University of Hong Kong.
Wang
C C
CC
Kuhn
H
H
Rathmann
J
J
Pang
C P
CP
Rogers
M S
MS
D000137:Acid-Base Imbalance / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000097:blood; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000097:blood; D028441:F2-Isoprostanes; D005260:Female; D005312:Fetal Blood; D006801:Humans; D007231:Infant, Newborn; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D007744:Obstetric Labor Complications / Q000175:diagnosis*; D018384:Oxidative Stress; D011247:Pregnancy; D011687:Purines / Q000097:blood; D014469:Umbilical Arteries / Q000378:metabolism*; D014471:Umbilical Veins / Q000378:metabolism*
D015415:Biomarkers; D028441:F2-Isoprostanes; D011687:Purines; C075750:8-epi-prostaglandin F2alpha; D008315:Malondialdehyde; D015237:Dinoprost