Cross-protection and reassortment studies with avian H2 influenza viruses.
In order to assess the degree of immune cross-protection among avian H2 influenza virus strains, mice were immunised with beta-propiolactone-inactivated virus preparations and infected intranasally with mouse-adapted variant of A/Black Duck/New Jersey/1580/78 (H2N3) strain. The experiments with 11 avian H2 strains revealed that both Eurasian and American H2 avian influenza viruses exhibit either high or moderate degree of cross-protection. The grouping of the strains in accordance with their cross-protection efficiency does not coincide with H2 phylogenetic branches. Several reassortant clones were obtained with the use of A/Pintail Duck/Primorie/695/76 (H2N3) strain and high-yield X-67 reassortant as parent viruses, among them a high-yield H2N3 reassortant. Taking into account the data on cross-protection among avian H2 strains, the high-yield H2N3 reassortant may be regarded as a prototype strain to be used for the preparation of killed vaccines in the case of a new appearance of avian H2 haemagglutinin in circulation in humans.
Kaverin
N V
NV
The DI Ivanovsky Institute of Virology RAMS, Moscow, Russia.
Smirnov
Y A
YA
Govorkova
E A
EA
Rudneva
I A
IA
Gitelman
A K
AK
Lipatov
A S
AS
Varich
N L
NL
Yamnikova
S S
SS
Makarova
N V
NV
Webster
R G
RG
Lvov
D K
DK
D000818:Animals; D002642:Chick Embryo; D003429:Cross Reactions; D007114:Immunization; D009980:Influenza A virus / Q000235:genetics* / Q000276:immunology* / Q000472:pathogenicity; D007252:Influenza Vaccines / Q000276:immunology*; D051379:Mice; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000517:prevention & control*; D016865:Reassortant Viruses / Q000235:genetics / Q000276:immunology*; D014613:Vaccines, Attenuated / Q000276:immunology
D007252:Influenza Vaccines; D014613:Vaccines, Attenuated