title: Comparative study of the coupling between topoisomerase I activity and high-mobility group proteins in E. coli and mammalian cells.
abstract: It is now well established that the HMG box DNA-binding motif can alter the topology of double-stranded DNA in several ways. Using the spermatid-specific tsHMG as a model protein of the HMG-1/-2 family, we have demonstrated that its expression in E. coli produces an increase in plasmid supercoiling density that is likely a consequence of its ability to constrain free supercoils in vivo. As demonstrated in vitro, stabilization of free DNA supercoils by tsHMG prevents topoisomerase I from gaining access to the template and could represent a mechanism for the apparent inhibition of topoisomerase I in bacteria. A similar modulation of eukaryotic topoisomerase I activity was not detected after expression of the tsHMG in mammalian cells. This differential response is discussed in terms of the marked difference in DNA packaging and accessibility of free supercoils in prokaryotic vs. eukaryotic cells.
authors.affiliation: Département de Biochimie, Faculté de Médicine, Université de Sherbrooke, Québec, Canada.
authors.forename: S
authors.initials: S
authors.lastname: Veilleux
authors.forename: N
authors.initials: N
authors.lastname: Caron
authors.forename: G
authors.initials: G
authors.lastname: Boissonneault
mesh_terms: D002460:Cell Line; D004261:DNA Replication; D004264:DNA Topoisomerases, Type I / Q000378:metabolism*; D004278:DNA, Superhelical / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005057:Eukaryotic Cells / Q000166:cytology / Q000378:metabolism; D017930:Genes, Reporter / Q000235:genetics; D006609:High Mobility Group Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D010957:Plasmids / Q000235:genetics; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D004278:DNA, Superhelical; D006609:High Mobility Group Proteins; D011993:Recombinant Fusion Proteins; D004264:DNA Topoisomerases, Type I