Mapping of leukaemia-associated breakpoints in chromosome band 3q21 using a newly established PAC contig.
Chromosome aberrations affecting band 3q21 are associated with a particularly poor prognosis in patients with acute myeloid leukaemia. To facilitate the molecular characterization of such rearrangements, we established a PAC contig covering the relevant genomic region. Using these PACs as probes in fluorescence in situ hybridization (FISH) experiments, we showed that a number of 3q21 breakpoints in patient samples map to a previously defined 'breakpoint cluster region'. Others, however, are located at varying distances centromeric of it. These results have important implications in the search for genes affected by 3q21 rearrangements.
Wieser
R
R
Institut für Medizinische Biologie der Universität Wien, Austria. rotraud.wieser@univie.ac.at
Volz
A
A
Schnittger
S
S
Jäger
U
U
Grüner
H
H
Meran
J G
JG
Wimmer
K
K
Ziegler
A
A
Fonatsch
C
C
D000208:Acute Disease; D000328:Adult; D000368:Aged; D019457:Chromosome Breakage / Q000235:genetics*; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics*; D020451:Contig Mapping / Q000379:methods*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007951:Leukemia, Myeloid / Q000235:genetics*; D008297:Male; D008875:Middle Aged