title: A randomized study comparing the effect of standard versus short incubation of sperm and oocyte on subsequent pregnancy and implantation rates following in vitro fertilization embryo transfer.
abstract: Some data suggest that some sperm are associated with a toxic product that lowers embryo implantation rates when these sperm come in contact with the zona pellucida. The possibility exists that shortening the incubation time could improve pregnancy rates. A randomized prospective study was initiated to evaluate pregnancy and implantation rates with transfer of embryos formed by conventional overnight vs short (2-h) co-incubation of sperm and oocytes. The clinical and viable pregnancy rates were 41 and 29% for conventional co-incubation versus 30 and 30% for short co-incubation. Implantation rates were also similar (17.9 vs 17.8%). Short incubation does not seem to impact any advantage over conventional co-incubation techniques in couples undergoing in vitro fertilization where the male factor is normal.
authors.affiliation: The University of Medicine and Dentistry of New Jersey, Camden, USA.
authors.forename: K
authors.initials: K
authors.lastname: Swenson
authors.forename: J H
authors.initials: JH
authors.lastname: Check
authors.forename: D
authors.initials: D
authors.lastname: Summers-Chase
authors.forename: J K
authors.initials: JK
authors.lastname: Choe
authors.forename: M L
authors.initials: ML
authors.lastname: Check
mesh_terms: D010064:Embryo Implantation; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D011247:Pregnancy; D018873:Pregnancy Rate; D013084:Sperm-Ovum Interactions