title: The lactoperoxidase system: the influence of iodide and the chemical and antimicrobial stability over the period of about 18 months.
abstract: The lactoperoxidase (LP) system is a natural antimicrobial system, the use of which has been suggested as a preservative in foods and pharmaceuticals. The effect of adding iodide to the LP system, the chemical stability and the change in antimicrobial effectiveness during storage was studied. Addition of iodide with thiocyanate increased the fungicidal and bactericidal effect against Candida albicans, Escherichia coli and Staphylococcus aureus. Pseudomonas aeruginosa showed the same inhibition in the LP system with iodide and without iodide. Storage of the LP system in completely filled airtight containers for 18 months caused a 35% loss of the initial thiocyanate concentration. The antimicrobial activity of this LP system was strong enough to kill inocula of 106 cfu ml-1 of the four test organisms within 2 h of contact time. During storage of the air-containing LP system, the concentration of thiocyanate was reduced below detection limit within 7 d, the concentrations of hypothiocyanite and hypoiodite within 350 d. After 516 d the antimicrobial activity of air-containing LP system was strong enough to kill inocula of 106 cfu ml-1 Ps. aeruginosa within 2 h, Staph. aureus within 4 h and Candida albicans and E. coli within 1 week of contact time.
authors.affiliation: Pharmaceutical Technology and Biopharmacy, University of Groningen, The Netherlands.
authors.forename: E H
authors.initials: EH
authors.lastname: Bosch
authors.forename: H
authors.initials: H
authors.lastname: van Doorne
authors.forename: S
authors.initials: S
authors.lastname: de Vries
mesh_terms: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects* / Q000254:growth & development; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development; D015169:Colony Count, Microbial; D004795:Enzyme Stability; D007454:Iodides / Q000737:chemistry*; D007784:Lactoperoxidase / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D013861:Thiocyanates / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D007454:Iodides; D013861:Thiocyanates; C014607:hypothiocyanite ion; D007784:Lactoperoxidase; C031760:thiocyanate