Phenotypic characteristics of Kaposi's sarcoma tumour cells derived from patch-, plaque- and nodular-stage lesions: analysis of cell cultures isolated from AIDS and non-AIDS patients and review of the literature.
BACKGROUND
Kaposi's sarcoma (KS) is commonly thought to be derived from endothelial cells because of the predominant expression of endothelial markers in KS lesions. However, the heterogeneity of the spindle-cell compartment makes the precise lineage relationship of KS tumour cells unclear. Cultured KS-derived spindle cells constitutively overexpress antiapoptotic proteins and exhibit invasive properties, which suggests that they may adequately represent the tumour cells of KS.


OBJECTIVE
We aimed to investigate the expression of a wide variety of immunohistochemical markers by spindle cells derived from patch-, plaque- and nodular-stage lesions from patients with iatrogenic, sporadic and acquired immune deficiency syndrome-related KS, and to review the data reported by other laboratories.


METHODS
Cells from six KS cell cultures derived from four subjects were examined by immunostaining.


RESULTS
Comparison of these data indicates that KS-derived spindle cells generally express myofibroblast antigens but lack endothelial and/or leucocyte markers.


CONCLUSIONS
As the myofibroblast phenotype is not the predominant feature of KS tissues, our findings further substantiate the view that the in vivo dominant endothelial population represents a reactive hyperplasia rather than the true KS tumour process.
Simonart
T
T
Departments of Dermatology, Immunology and Internal Medicine, Erasme University Hospital, 808 Route de Lennik, B-1070 Brussels, Belgium. simonart@erasme.ulb.ac.be
Hermans
P
P
Schandene
L
L
Van Vooren
J P
JP
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications*; D004727:Endothelium / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism*; D009367:Neoplasm Staging; D010641:Phenotype; D012514:Sarcoma, Kaposi / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D012878:Skin Neoplasms / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D014407:Tumor Cells, Cultured
D009363:Neoplasm Proteins