Gangliosides induce cell apoptosis in the cytotoxic line CTLL-2, but not in the promyelocyte leukemia cell line HL-60.
Gangliosides induce apoptosis in the cells of the IL-2-dependent cytotoxic mouse line CTLL-2. Upon incubation with gangliosides for 24 h, their effect resulting in appearance of apoptotic cells, falls in a series GM2 > GM3 > GM1 > GD1a > GD1b > GT1b. In the presence of rIL-2, apoptosis induced by GM1 is suppressed, whereas that induced by GM2 is enhanced (the effect of intracellular agent C2-Cer is independent of this cytokine). The GM1-induced apoptosis is cancelled by the caspase I inhibitor. The gangliosides under study are not able to induce apoptosis in the promyelocyte leukemia cell line HL-60. Physiological aspects of the phenomenon found are discussed.
Molotkovskaya
I M
IM
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow. immol@ibch.siobc.ras.ru
Kholodenko
R V
RV
Zelenova
N A
NA
Sapozhnikov
A M
AM
Mikhalev
I I
II
Molotkovsky
J G
JG
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D061945:Caspase Inhibitors; D002460:Cell Line; D005434:Flow Cytometry; D005732:Gangliosides / Q000145:classification / Q000502:physiology*; D018922:HL-60 Cells; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D051379:Mice; D009842:Oligopeptides / Q000494:pharmacology; D015398:Signal Transduction; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects / Q000502:physiology*
D061945:Caspase Inhibitors; D005732:Gangliosides; D007376:Interleukin-2; D009842:Oligopeptides; C096429:L 709049