Comparison of inhibitory effects of local anesthetics on immune functions of neutrophils.
Immunological effects of a variety of local anesthetics on adhesion, phagocytosis, and the production of superoxide anion and hydrogen peroxide by neutrophils were compared. Neutrophils were isolated by peritoneal lavage from rats, 4 h after injection of 1% glycogen. Lidocaine, mepivacaine, procaine, prilocaine and tetracaine at 1 mg/ml inhibited adhesion, phagocytosis, and the production of superoxide anion and hydrogen peroxide in neutrophils. Moreover, lidocaine, mepivacaine, procaine and prilocaine at 0.1 mg/ml inhibited the production of superoxide anion and hydrogen peroxide but not adhesion or phagocytosis. In contrast, tetracaine at 0.1 mg/ml inhibited phagocytosis, and the production of superoxide anion and hydrogen peroxide but not adhesion. At 0.01 mg/ml, however, tetracaine inhibited the production of superoxide anion and hydrogen peroxide; in contrast, other drugs failed to affect neutrophil function. These results suggest that the local anesthetics may affect adhesion, phagocytosis, and the production of superoxide anion and hydrogen peroxide by neutrophils.
Azuma
Y
Y
Department of Pharmacology, Osaka Dental University, 8-1 Kuzuhahanazono-cho, Hirakata, 573-1121, Osaka, Japan. aazuma@cc.osaka-dent.ac.jp
Shinohara
M
M
Wang
P L
PL
Suese
Y
Y
Yasuda
H
H
Ohura
K
K
D000779:Anesthetics, Local / Q000494:pharmacology*; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003864:Depression, Chemical; D006861:Hydrogen Peroxide / Q000378:metabolism; D007111:Immunity, Cellular / Q000187:drug effects*; D066298:In Vitro Techniques; D008012:Lidocaine / Q000494:pharmacology; D008297:Male; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology*; D010587:Phagocytosis / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013481:Superoxides / Q000378:metabolism
D000779:Anesthetics, Local; D013481:Superoxides; D008012:Lidocaine; D006861:Hydrogen Peroxide