Characterization of hch, the Podospora anserina homolog of the het-c heterokaryon incompatibility gene of Neurospora crassa.
The het-c locus controls heterokaryon formation in Neurospora crassa. It is subject to balancing selection operating to maintain polymorphism at that locus in natural populations. We have isolated hch, the het-c homolog from the related species Podospora anserina (hch for het-c homolog), in order to determine if this gene also functions as a het gene in that species. The het-c and hch sequences are highly similar but differ in the region defining allele specificity in N. crassa het-c. Analysis of hch variability in 11 natural P. anserina isolates with different het genotypes revealed no polymorphism. This suggested that hch does not function as a het gene. However, heterologous expression of the N. crassa het-cPA allele in P. anserina triggers a growth defect reminiscent of the het-c incompatibility reaction.
Saupe
S J
SJ
Laboratoire de Génétique Moléculaire des Champignons Filamenteux, IBGC UMR CNRS, Bordeaux, France. sven.saupe@ibgc.u-bordeaux2.fr
Clavé
C
C
Sabourin
M
M
Bégueret
J
J
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D005838:Genotype; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000235:genetics*; D014176:Protein Biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D020037:Sordariales / Q000166:cytology / Q000235:genetics* / Q000254:growth & development; D014170:Transformation, Genetic
D005656:Fungal Proteins; D011994:Recombinant Proteins