Ectodermal markers delineate the neural fold interface during avian neurulation.
The formation and morphogenesis of the neural folds are important processes underlying neurulation. We showed previously that these processes comprise four key events in avian embryos: epithelial ridging, kinking, delamination, and apposition. Collectively, these events establish the paired, bilaminar neural folds, which fuse in the dorsal midline during late neurulation to close the neural groove and to establish the neural tube. Here, we use an antisense riboprobe for a new gene called Plato, as well as an antibody for a previously cloned transcription factor, AP-2, as markers to identify critical subpopulations of ectodermal cells during the formation and morphogenesis of the avian neural folds. Plato antisense riboprobe marks the cranial neural ectoderm and premigratory cranial neural crest cells, whereas AP-2 antibody marks the epidermal ectoderm and the early migratory neural crest. We show that subpopulations of ectodermal cells at the forebrain and midbrain levels undergo considerable rearrangement within the neural fold transition zone, which redistributes incipient neural crest cells from the neural ectodermal side of the forming neural fold interface to the epidermal ectodermal side. Additionally, we show that Plato and AP-2 provide useful markers for delineating the incipient neural fold interface.
Lawson
A
A
Department of Neurobiology and Anatomy, University of Utah School of Medicine, Salt Lake City, Utah 84132, USA.
Colas
J F
JF
Schoenwolf
G C
GC
D000818:Animals; D015415:Biomarkers; D001717:Birds / Q000196:embryology*; D004475:Ectoderm / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000378:metabolism / Q000502:physiology; D015870:Gene Expression / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009420:Nervous System / Q000196:embryology*; D013997:Time Factors; D014018:Tissue Distribution; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D015415:Biomarkers; D009419:Nerve Tissue Proteins; D014157:Transcription Factors