title: Sequence analysis of Pns11, a nonstructural protein of rice gall dwarf virus, and its expression and detection in infected rice plants and vector insects.
abstract: The nucleotide sequence of genome segment S11 of rice gall dwarf virus (RGDV), a member of Phytoreovirus, was determined. The segment encodes a putative protein of 40 kDa that exhibits approximately 37% homology at the amino acid level to the nonstructural proteins Pns10 of rice dwarf and wound tumor viruses, which are other members of Phytoreovirus. A band of a protein with an apparent molecular mass of 40 kDa was specifically detected in an analysis of cells transfected with S11 cDNA. An antiserum raised against this protein reacted with a protein of approximately 40kDa after fractionation by SDS-PAGE of materials prepared from infected plants and from viruliferous vector insects. However, the antiserum did not react with purified viral proteins. These results suggest that S11 encodes a nonstructural protein of RGDV. This protein was named Pns11.
authors.affiliation: National Agriculture Research Center, Tsukuba, Ibaraki, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Moriyasu
authors.forename: K
authors.initials: K
authors.lastname: Ishikawa
authors.forename: A
authors.initials: A
authors.lastname: Kikuchi
authors.forename: S
authors.initials: S
authors.lastname: Imanishi
authors.forename: S
authors.initials: S
authors.lastname: Tomita
authors.forename: K
authors.initials: K
authors.lastname: Akutsu
authors.forename: T
authors.initials: T
authors.lastname: Omura
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D007303:Insect Vectors / Q000378:metabolism*; D008969:Molecular Sequence Data; D012275:Oryza / Q000821:virology*; D010940:Plant Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D012087:Reoviridae / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D014764:Viral Proteins
chemical_list: D010940:Plant Proteins; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; C070922:dwarf phytoreovirus segment 11 protein, rice dwarf phytoreovirus