title: The effect of apolipoprotein B xbaI polymorphism on plasma lipid response to dietary fat.
abstract: OBJECTIVE
  Lipid response to dietary fat and cholesterol is, to a large extent, genetically controlled. Apolipoprotein B (apo B) plays a dominant role in cholesterol homeostasis. Several polymorphic sites within or adjacent to the gene locus for apo B have been detected. The X+ allele of the XbaI restriction fragment polymorphism of the apo B gene has been found to be associated with higher serum cholesterol and/or triglyceride levels. In order to study the influence of this mutation on the plasma lipid response in diets of varying fat content, 72 healthy male subjects were studied, 21 X- X- (X-) and 51 X+ (X+ X- or X+ X+).
  
  
  RESULTS
  These subjects followed three consecutive 28-day diet periods: one rich in saturated fats (SAT diet; 38% fat, 20% saturated); a National Cholesterol Education Program type I diet (NCEP-I diet) (28% fats, < 10% saturated); and a third monounsaturated (MUFA diet) (38% fats, 22% monounsaturated). The different genotypes can be observed to have significant effects on total and LDL cholesterol concentrations (P < 0.017). X+ individuals had higher levels of total and LDL cholesterol after the consumption of a SAT diet (P < 0.012; P < 0.006, respectively), NCEP diet (P < 0.060; P < 0.054, respectively) and MUFA diet (P < 0.022; P < 0.042, respectively) in comparison with X- individuals. A significant interaction between genotypes and dietary effects was observed for diet-induced changes in plasma triglycerides (P < 0.032). Significant decreases in the absolute values of triglyceride concentrations (-0.18 mmol L(-1), P < 0.024) were noted in the X- subjects after the high intake of a MUFA diet, while no significant differences were observed in the X+ individuals (0.006 mmol L(-1), P < 0.858).
  
  
  CONCLUSIONS
  Our results suggest that the total triglyceride response to diet is influenced by the apo B XbaI polymorphism.
authors.affiliation: Servicio de Medicina Interna, Hospital Alto Guadalquivir, Andujar, Spain.
authors.forename: J
authors.initials: J
authors.lastname: López-Miranda
authors.forename: C
authors.initials: C
authors.lastname: Marín
authors.forename: P
authors.initials: P
authors.lastname: Castro
authors.forename: P
authors.initials: P
authors.lastname: Gómez
authors.forename: A
authors.initials: A
authors.lastname: González-Amieva
authors.forename: E
authors.initials: E
authors.lastname: Paz
authors.forename: D
authors.initials: D
authors.lastname: Bravo
authors.forename: J M
authors.initials: JM
authors.lastname: Ordovas
authors.forename: J
authors.initials: J
authors.lastname: Jimenez-Pereperez
authors.forename: F
authors.initials: F
authors.lastname: Pérez-Jiménez
mesh_terms: D000328:Adult; D000483:Alleles; D016632:Apolipoprotein A-I / Q000097:blood; D001055:Apolipoproteins B / Q000235:genetics* / Q000378:metabolism; D015992:Body Mass Index; D002784:Cholesterol / Q000097:blood; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D015930:Diet Records; D004041:Dietary Fats / Q000378:metabolism*; D005227:Fatty Acids / Q000097:blood; D005838:Genotype; D006801:Humans; D008055:Lipids / Q000097:blood*; D008297:Male; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D016632:Apolipoprotein A-I; D001055:Apolipoproteins B; D004041:Dietary Fats; D005227:Fatty Acids; D008055:Lipids; D002784:Cholesterol; C025580:endodeoxyribonuclease XBAI; D015252:Deoxyribonucleases, Type II Site-Specific