In vitro and in vivo uric acid lowering by artificial cells containing microencapsulated genetically engineered E. coli DH5 cells.
Increase in systemic uric acid occurs in renal insufficiency, gout, chemotherapy, and other diseases. Dialysis can lower this metabolite but is expensive. The use of drugs can, sometime, result in side effects. Therefore, a suitable affordable method for this is required. In this article, for the first time, we report the use of artificial cells containing micro encapsulated genetically engineered E. Coli DH5 cells for lowering uric acid in vitro and in vivo. Results show that this novel approach has the ability to significantly lower uric acid from 84.80 +/- 3.40 mg/dl to 9.32 +/- 0.05 mg/dl in vitro and from the plasma of the experimental animals from the control levels of 71.00 +/- 27.49 mg/dl to 20.33 +/- 17.92 mg/dl in vivo. Continued daily oral administration maintained the plasma uric acid concentration of experimental uremic rats to the normal plasma uric acid level range during the entire test period.
Prakash
S
S
Artificial Cells and Organs Research Center, Faculty of Medicine, Mc Gill University, Montreal, Quebec, Canada.
Chang
T M
TM
D000818:Animals; D002477:Cells / Q000378:metabolism / Q000382:microbiology; D004926:Escherichia coli; D005818:Genetic Engineering; D008297:Male; D008567:Membranes, Artificial; D051381:Rats; D017208:Rats, Wistar; D014527:Uric Acid / Q000097:blood*
D008567:Membranes, Artificial; D014527:Uric Acid