Virtual cloning and physical mapping of a human T-box gene, TBX4.
The Tbx2/3/4/5 subfamily is one of the largest subgroupings within the T-box gene family, the members of which encode developmentally critical transcription factors. TBX4, a human member of the Tbx2/3/4/5 subfamily, has been identified and characterized from a high-throughput genomic sequence. The genomic organization of TBX4 was elucidated by computational sequence analysis, and the putative cDNA sequence was assembled. The genomic organization of TBX4 is very similar to that of TBX5, as is the situation for TBX2 and TBX3. The physical configuration of the TBX4-TBX2 cluster on human chromosome 17q21-q22 is similar to that of the TBX5-TBX3 cluster on chromosome 12q23-q24. The assembled TBX4 cDNA sequence was searched against the EST databases, and a TBX4 EST was identified.
Yi
C H
CH
Institute of Genetics, University of Nottingham, Queen's Medical Centre, United Kingdom.
Russ
A
A
Brook
J D
JD
D001483:Base Sequence; D022201:Chromosomes, Artificial; D002876:Chromosomes, Bacterial; D002886:Chromosomes, Human, Pair 17; D003001:Cloning, Molecular; D019295:Computational Biology; D016208:Databases, Factual; D005091:Exons; D020224:Expressed Sequence Tags; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D005810:Multigene Family; D020161:Physical Chromosome Mapping; D020825:T-Box Domain Proteins / Q000235:genetics*
C097348:T-Box Domain Protein 2; D020825:T-Box Domain Proteins; C103172:T-box transcription factor 5; C106884:TBX3 protein, human; C491193:TBX4 protein, human; C491460:Tbx3 protein, mouse