Fluorescent studies on the binding-Ca2 + in fibrinolytic principle separated from snake venom.
By using equilibrium dialysis, atomic absorption spectrometry, fluorescence titration and determination of fluorescence lifetime, it can be determined that each fibrinolytic principle (FP) molecule contains one Ca2+-binding site and one Ca2+ ion, which can be substituted by a Tb3+ ion completely. The intramolecular energy transfer between Tb3+ and the tryptophan (Trp) residue in FP has been investigated through fluorescence spectroscopy. In the FP molecule, the excited energy can transfer from the Trp residue as an energy donor to the Tb3+ ion substituted as an acceptor. The distance between Tb3+ and the Trp residue, approximately 0.38 nm, has been calculated with the experimental data and Forster theory.
Wang
S
S
Department of Chemistry, University of Science and Technology of China, Hefei, Anhui Province, PR China.
Liu
Q
Q
Chen
Z
Z
Zhang
Y
Y
D017836:Agkistrodon; D000818:Animals; D002118:Calcium / Q000032:analysis*; D004735:Energy Transfer; D005343:Fibrinolytic Agents / Q000737:chemistry* / Q000302:isolation & purification; D008968:Molecular Conformation; D012910:Snake Venoms / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013725:Terbium / Q000737:chemistry; D017186:Titrimetry; D014364:Tryptophan / Q000737:chemistry
D005343:Fibrinolytic Agents; D012910:Snake Venoms; D013725:Terbium; D014364:Tryptophan; D002118:Calcium