title: A novel oxylipin-associated 'ghosting' phenomenon in yeast flocculation.
abstract: Research on the distribution of oxylipins (3-hydroxy fatty acids) in flocculant strains of the yeast Saccharomyces cerevisiae led to the uncovering of a novel 'ghosting' phenomenon observed during assumed lectin-mediated aggregation. We found that intracellular oxylipin-containing osmiophilic layers migrate through yeast cell walls in a 'ghostlike' fashion without visually affecting the cell wall structure or the layers. This migration resulted in the binding of these layers to cell walls of adjacent cells. Consequently, 'ghosting' seems a prerequisite for flocculation to occur. However, 'ghosting' alone may not be sufficient to ensure flocculation.
authors.affiliation: Department of Microbiology and Biochemistry, University of the Orange Free State, Bloemfontein, South Africa. Kockjlf@micro.nw.uovs.ac.za
authors.forename: J L
authors.initials: JL
authors.lastname: Kock
authors.forename: P
authors.initials: P
authors.lastname: Venter
authors.forename: D P
authors.initials: DP
authors.lastname: Smith
authors.forename: P W
authors.initials: PW
authors.lastname: Van Wyk
authors.forename: P J
authors.initials: PJ
authors.lastname: Botes
authors.forename: D J
authors.initials: DJ
authors.lastname: Coetzee
authors.forename: C H
authors.initials: CH
authors.lastname: Pohl
authors.forename: A
authors.initials: A
authors.lastname: Botha
authors.forename: K H
authors.initials: KH
authors.lastname: Riedel
authors.forename: S
authors.initials: S
authors.lastname: Nigam
mesh_terms: D002473:Cell Wall / Q000648:ultrastructure*; D005227:Fatty Acids / Q000302:isolation & purification*; D005427:Flocculation; D006880:Hydroxy Acids / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D005227:Fatty Acids; D006880:Hydroxy Acids