title: Isolation and characterization of low-indole-3-acetic acid-producing mutants from Bradyrhizobium elkanii.
abstract: We isolated 11 low-indole-3-acetic acid (IAA)-producing mutants of Bradyrhizobium elkanii by Tn5 mutagenesis. The amount of IAA produced by each mutant was 2.2-13.6% of that of the wild-type. It was found by resting cell reactions that the biosynthetic step to convert indole-3-pyruvic acid to indole-3-acetaldehyde was blocked in all the mutants.
authors.affiliation: Biotechnology Research Center, The University of Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yagi
authors.forename: T
authors.initials: T
authors.lastname: Matsumoto
authors.forename: T
authors.initials: T
authors.lastname: Chujo
authors.forename: H
authors.initials: H
authors.lastname: Nojiri
authors.forename: T
authors.initials: T
authors.lastname: Omori
authors.forename: K
authors.initials: K
authors.lastname: Minamisawa
authors.forename: M
authors.initials: M
authors.lastname: Nishiyama
authors.forename: H
authors.initials: H
authors.lastname: Yamane
mesh_terms: D020369:Bradyrhizobium / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D004269:DNA, Bacterial / Q000032:analysis; D007210:Indoleacetic Acids / Q000378:metabolism*; D016296:Mutagenesis; D015183:Restriction Mapping / Q000379:methods; D019895:Transposases
chemical_list: D004269:DNA, Bacterial; D007210:Indoleacetic Acids; C030737:indoleacetic acid; D019895:Transposases