Cholinergic modulation of inhibitory avoidance impairment induced by paradoxical sleep deprivation.
1. Male Wistar rats were submitted to paradoxical sleep deprivation for 96 hr by a modified multiple platform technique. 2. Training of step-through inhibitory avoidance was performed immediately after the last day of paradoxical sleep deprivation. Twenty-four hr after training the animals were submitted to the retention test. 3. In Experiment 1, pilocarpine (4 mg/kg, i.p.) or atropine (4 mg/kg, i.p.) were administered daily during the paradoxical sleep deprivation period. Pilocarpine, but not atropine, reversed the impairment induced by PS deprivation. 4. In Experiment 2, pilocarpine (4, 8 and 12 mg/kg, i.p.) was injected 1 hr before training in order to verify if the reversal of memory impairment was an effect secondary to residual enhanced blood levels of pilocarpine during training. Acute treatment with pilocarpine, in any dose, did not reverse the impairment produced by paradoxical sleep deprivation 5. Activation of the cholinergic system during the period of deprivation is able to prevent memory deficits induced by paradoxical sleep deprivation.
Bueno
O F
OF
Department of Psychobiology, Universidade Federal de São Paulo, SP, Brazil. mgabi@psicobio.epm.br
Oliveira
G M
GM
Lobo
L L
LL
Morais
P R
PR
Melo
F H
FH
Tufik
S
S
D000818:Animals; D001285:Atropine / Q000008:administration & dosage / Q000494:pharmacology; D001362:Avoidance Learning; D008297:Male; D018721:Muscarinic Agonists / Q000008:administration & dosage / Q000494:pharmacology; D018727:Muscarinic Antagonists / Q000008:administration & dosage / Q000494:pharmacology; D010862:Pilocarpine / Q000008:administration & dosage / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D011950:Receptors, Cholinergic / Q000502:physiology; D012892:Sleep Deprivation; D012895:Sleep, REM / Q000502:physiology*
D018721:Muscarinic Agonists; D018727:Muscarinic Antagonists; D011950:Receptors, Cholinergic; D010862:Pilocarpine; D001285:Atropine