An antibody assay predictive of BRCA1 mutations in ovarian tumors and normal tissue.
To determine whether immunohistochemistry can identify BRCA1 mutations, immunohistochemical (IH) analysis was undertaken on paraffin sections of paired ovarian cancer and normal tissue using antibodies against both terminal regions of the BRCA1 protein. Ten patients at risk for BRCA1 mutations were studied. The results of BRCA1 mutation analysis and IH were compared. In tumor, IH correctly identified the presence or absence of loss of heterozygosity in all specimens. In all uninvolved specimens, IH correctly identified the presence or absence of a germline mutation. The sensitivity, specificity, positive and negative predictive values were 100% suggesting promise as a rapid and inexpensive screen.
Byrne
T J
TJ
Department of Plant & Soil Sciences, University of Massachusetts, Amherst, MA, USA.
Reece
M T
MT
Adams
L A
LA
Lane
M A
MA
Cohn
G M
GM
D000328:Adult; D000906:Antibodies; D019313:BRCA1 Protein / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D004252:DNA Mutational Analysis; D005260:Female; D019398:Genes, BRCA1 / Q000235:genetics*; D018095:Germ-Line Mutation; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology; D010053:Ovary / Q000378:metabolism / Q000502:physiology; D016612:Paraffin Embedding; D010446:Peptide Fragments / Q000276:immunology*; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
D000906:Antibodies; D019313:BRCA1 Protein; D010446:Peptide Fragments