title: Effects of testosterone and 17, beta-estradiol on the polyamine metabolism in cultivated normal rat kidney epithelial cells.
abstract: Ornithine decarboxylase (ODC) and diamine oxidase (DAO) are important enzymes involved in the metabolism of polyamines (putrescine, spermidine and spermine). The influence of testosterone (T) and 17, beta-estradiol (E2) on the activity of ODC and DAO was examined in cultivated normal rat kidney (NRK) epithelial cells. The results showed an increase in enzyme activities 4 hours or 12 hours after hormonal treatment. Both T and E2 led to a significant increase (1.6-fold) in ODC protein level as compared to the controls. Cellular concentration of spermidine and spermine increased (2.2- and 2.6-fold respectively) 4 hours after T addition. A higher levels in concentrations of putrescine (1.4-fold) and spermine (1.5-fold) 12 hours after E2 treatment were observed. These results suggest that the biosynthesis and terminal oxidation of the polyamines in NRK epithelial cells are androgen- and estrogen-mediated and depend on the hormonal sensitivity of the cells.
authors.affiliation: Department of Human and Animal Physiology, Faculty of Biology, Sofia University, Bulgaria.
authors.forename: I
authors.initials: I
authors.lastname: Jotova
authors.forename: C
authors.initials: C
authors.lastname: Wang
authors.forename: A
authors.initials: A
authors.lastname: Tabib
authors.forename: O
authors.initials: O
authors.lastname: Dimitrov
authors.forename: U
authors.initials: U
authors.lastname: Bachrach
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D002457:Cell Extracts / Q000737:chemistry; D002478:Cells, Cultured; D004847:Epithelial Cells; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D007668:Kidney / Q000166:cytology; D008297:Male; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D011073:Polyamines / Q000378:metabolism*; D011700:Putrescine / Q000096:biosynthesis / Q000378:metabolism; D051381:Rats; D013095:Spermidine / Q000096:biosynthesis / Q000378:metabolism; D013096:Spermine / Q000096:biosynthesis / Q000378:metabolism; D013739:Testosterone / Q000494:pharmacology*
chemical_list: D002457:Cell Extracts; D011073:Polyamines; D013096:Spermine; D013739:Testosterone; D004958:Estradiol; D006631:Amine Oxidase (Copper-Containing); D009955:Ornithine Decarboxylase; D013095:Spermidine; D011700:Putrescine