title: Cytological and molecular evidence that the whitefly-transmitted cucumber vein yellowing virus is a tentative member of the family Potyviridae.
abstract: Cucumber vein yellowing virus (CVYV) is widespread in cucurbits in the Middle East. CVYV has filamentous particles and is transmitted by Bemisia tabaci by the semi-persistent mode. It has not yet been assigned to a specific genus or family. Ultramicroscopic observations revealed numerous cylindrical cytoplasmic inclusions in melon and cucumber cells infected by CVYV isolates from Israel and Jordan. Depending on the section orientation, the inclusions appeared as pinwheels or as bundles. In addition, a 1.9 kb DNA fragment was amplified by RT-PCR from CVYV-infected plant extracts using primers designed to detect all potyvirids. Sequence comparisons with the amplified fragment indicated that CVYV is more closely related to Sweet potato mild mottle virus than to any other virus in the family Potyviridae: These results suggest that CVYV can be considered as a tentative new member of the genus Ipomovirus:, family Potyviridae:
authors.affiliation: Station de Pathologie Végétale, INRA, Domaine Saint Maurice, BP 94, 84143 Montfavet cedex, France1.
authors.forename: H
authors.initials: H
authors.lastname: Lecoq
authors.affiliation: Station de Pathologie Végétale, INRA, Domaine Saint Maurice, BP 94, 84143 Montfavet cedex, France1.
authors.forename: C
authors.initials: C
authors.lastname: Desbiez
authors.affiliation: Station de Pathologie Végétale, INRA, Domaine Saint Maurice, BP 94, 84143 Montfavet cedex, France1.
authors.forename: B
authors.initials: B
authors.lastname: Delécolle
authors.affiliation: Department of Virology, Agricultural Research Organization, The Volcani Center, POB 6, Bet Dagan 50250, Israel2.
authors.forename: S
authors.initials: S
authors.lastname: Cohen
authors.affiliation: Horticulture and Plant Protection Department, Faculty of Agriculture, University of Jordan, Amman, Jordan3.
authors.forename: A
authors.initials: A
authors.lastname: Mansour
mesh_terms: D000595:Amino Acid Sequence; D019662:Cucurbitaceae / Q000821:virology*; D004279:DNA, Viral / Q000737:chemistry; D007557:Israel; D007597:Jordan; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D010936:Plant Extracts / Q000737:chemistry; D019176:Potyviridae / Q000145:classification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D004279:DNA, Viral; D010936:Plant Extracts; D014764:Viral Proteins