title: Low-potassium dextran solution ameliorates reperfusion injury of the lung and protects surfactant function.
abstract: OBJECTIVE
  This study was designed to compare the effect of lung preservation with low-potassium dextran solution and Euro-Collins solution on reperfusion injury and surfactant function by using an in situ model of warm ischemia.
  
  
  METHODS
  The left lungs of 6 minipigs were selectively perfused with Euro-Collins solution. In an additional 6 animals low-potassium dextran solution was used for flush perfusion. After 90 minutes of warm ischemia, the lungs were reperfused, and the contralateral pulmonary artery and bronchus were clamped. Hemodynamic and respiratory measurements were obtained for 7 hours of reperfusion. Surface tension of bronchoalveolar lavage and surfactant small and large aggregates were determined before perfusion (right lung) and after 2 hours of reperfusion (left lung).
  
  
  RESULTS
  In the group receiving Euro-Collins solution, right heart failure developed within 215 +/- 39 minutes of reperfusion. An increase in minimal surface tension (P =.03), surfactant small aggregates/large aggregates ratio (P =.003), and bronchoalveolar lavage protein content (P =.012) were found after 2 hours of reperfusion. In the group receiving low-potassium dextran solution, all minipigs survived (P =.0001). Dynamic lung compliance (P =.034) and oxygen tension/inspired oxygen fraction ratios were higher (P =. 0001). Lung water content was lower (P =.049). The increase of minimal surface tension (P =.02) and bronchoalveolar lavage protein concentration (P =.015) were significantly less.
  
  
  CONCLUSIONS
  Preservation of the lung with Euro-Collins solution leads to a reduction of physical surfactant function during reperfusion. Low-potassium dextran solution protects surfactant function and metabolism, thereby reducing reperfusion injury of the lung.
authors.affiliation: Division of Thoracic and Cardiovascular Surgery and the Department of Pneumology, Hannover Medical School, Hannover, Germany. strueber@thg.mhhannover.de
authors.forename: M
authors.initials: M
authors.lastname: Strüber
authors.forename: J M
authors.initials: JM
authors.lastname: Hohlfeld
authors.forename: S
authors.initials: S
authors.lastname: Fraund
authors.forename: P
authors.initials: P
authors.lastname: Kim
authors.forename: G
authors.initials: G
authors.lastname: Warnecke
authors.forename: A
authors.initials: A
authors.lastname: Haverich
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D003911:Dextrans / Q000494:pharmacology*; D006982:Hypertonic Solutions / Q000494:pharmacology; D008168:Lung / Q000098:blood supply* / Q000502:physiology; D019188:Organ Preservation Solutions / Q000494:pharmacology; D010477:Perfusion; D011506:Proteins / Q000032:analysis; D011663:Pulmonary Surfactants / Q000032:analysis / Q000502:physiology*; D011897:Random Allocation; D015427:Reperfusion Injury / Q000517:prevention & control*; D013500:Surface Tension; D013552:Swine; D013556:Swine, Miniature; D014021:Tissue Preservation / Q000379:methods
chemical_list: D003911:Dextrans; C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D019188:Organ Preservation Solutions; D011506:Proteins; D011663:Pulmonary Surfactants