title: The domineering non-autonomy of frizzled and van Gogh clones in the Drosophila wing is a consequence of a disruption in local signaling.
abstract: The frizzled (fz) gene is required for the development of distally pointing hairs on the Drosophila wing. It has been suggested that fz is needed for the propagation of a signal along the proximal distal axis of the wing. The directional domineering non-autonomy of fz clones could be a consequence of a failure in the propagation of this signal. We have tested this hypothesis in two ways. In one set of experiments we used the domineering non-autonomy of fz and Vang Gogh (Vang) clones to assess the direction of planar polarity signaling in the wing. prickle (pk) mutations alter wing hair polarity in a cell autonomous way, so pk cannot be altering a global polarity signal. However, we found that pk mutations altered the direction of the domineering non-autonomy of fz and Vang clones, arguing that this domineering non-autonomy is not due to an alteration in a global signal. In a second series of experiments we ablated cells in the pupal wing. We found that a lack of cells that could be propagating a long-range signal did not alter hair polarity. We suggest that fz and Vang clones result in altered levels of a locally acting signal and the domineering non-autonomy results from wild-type cells responding to this abnormal signal.
authors.affiliation: Biology Department and Cancer Center, University of Virginia, Charlottesville 22903, USA. pna@virginia.edu
authors.forename: P N
authors.initials: PN
authors.lastname: Adler
authors.forename: J
authors.initials: J
authors.lastname: Taylor
authors.forename: J
authors.initials: J
authors.lastname: Charlton
mesh_terms: D000483:Alleles; D000818:Animals; D019521:Body Patterning / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D004330:Drosophila / Q000235:genetics* / Q000254:growth & development*; D029721:Drosophila Proteins; D051157:Frizzled Receptors; D017344:Genes, Insect; D060588:LIM Domain Proteins; D008565:Membrane Proteins / Q000235:genetics*; D008954:Models, Biological; D009030:Mosaicism; D009154:Mutation; D011679:Pupa / Q000254:growth & development; D043562:Receptors, G-Protein-Coupled; D015398:Signal Transduction; D014921:Wings, Animal / Q000254:growth & development*
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D051157:Frizzled Receptors; D060588:LIM Domain Proteins; D008565:Membrane Proteins; D043562:Receptors, G-Protein-Coupled; C058784:fz protein, Drosophila; C121600:pk protein, Drosophila