Isolation and characterization of low-indole-3-acetic acid-producing mutants from Bradyrhizobium elkanii.
We isolated 11 low-indole-3-acetic acid (IAA)-producing mutants of Bradyrhizobium elkanii by Tn5 mutagenesis. The amount of IAA produced by each mutant was 2.2-13.6% of that of the wild-type. It was found by resting cell reactions that the biosynthetic step to convert indole-3-pyruvic acid to indole-3-acetaldehyde was blocked in all the mutants.
Yagi
K
K
Biotechnology Research Center, The University of Tokyo, Japan.
Matsumoto
T
T
Chujo
T
T
Nojiri
H
H
Omori
T
T
Minamisawa
K
K
Nishiyama
M
M
Yamane
H
H
D020369:Bradyrhizobium / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D004269:DNA, Bacterial / Q000032:analysis; D007210:Indoleacetic Acids / Q000378:metabolism*; D016296:Mutagenesis; D015183:Restriction Mapping / Q000379:methods; D019895:Transposases
D004269:DNA, Bacterial; D007210:Indoleacetic Acids; C030737:indoleacetic acid; D019895:Transposases