Replacement of the phosphodiester bond between U4 and G5 in the U-turn of a chemically modified hammerhead ribozyme by an amide bond.
The phosphodiester bond between U4 and G5 in the U-turn of a chemically modified hammerhead ribozyme was substituted by an amide backbone without compromising the ribozyme's cleavage activity. Furthermore, the modified ribozyme proved to be completely stable against endonucleolytic digestion at this position.
Dunkel
M
M
Nigu Chemie GmbH, Postfach, Waldkraiburg, Germany. mdunkel@nigu.de
Reither
V
V
D000577:Amides / Q000737:chemistry*; D001665:Binding Sites; D002384:Catalysis; D004720:Endonucleases / Q000378:metabolism; D019791:Guanidine / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D016337:RNA, Catalytic / Q000737:chemistry* / Q000378:metabolism*; D013329:Structure-Activity Relationship; D014529:Uridine / Q000031:analogs & derivatives* / Q000138:chemical synthesis
D000577:Amides; D016337:RNA, Catalytic; D004720:Endonucleases; D019791:Guanidine; D014529:Uridine