title: Fibroblast growth factor-10: a stromal mediator of epithelial function in the ovine uterus.
abstract: Fibroblast growth factor-10 (FGF-10) is a stromal-derived paracrine growth factor considered to be important during embryogenesis; however, its expression by cells in the female reproductive tract has not been investigated. Therefore, an ovine FGF-10 cDNA was cloned from an ovine endometrial cDNA library to investigate expression and potential paracrine characteristics of FGF-10 in the ovine uterus. The ovine FGF-10 cDNA encodes a protein of 213 amino acids and possesses an unusually long 5' untranslated region (UTR). In situ hybridization demonstrated that ovine FGF-10 mRNA was expressed by endometrial stromal cells and by mesenchymal cells of the chorioallantoic placenta. The mRNA for FGF-7, a homologue of FGF-10, was localized in the tunica muscularis of blood vessels in endometrium and myometrium. In contrast, FGF receptor 2IIIb, the high-affinity receptor for both FGF-10 and FGF-7, was expressed exclusively in luminal epithelium, glandular epithelium, and placental trophectoderm. The in vivo spatial expression pattern suggests that FGF-10 is a novel endometrial stromal cell-derived mediator of uterine epithelial and conceptus trophectodermal functions. The nonoverlapping spatial patterns of expression for FGF-10 and FGF-7 in ovine uterus and conceptus suggest independent roles in uterine function and conceptus development.
authors.affiliation: Center for Animal Biotechnology and Genomics, and Department of Animal Science, Texas A&M University,College Station, Texas 77843-2471, USA.
authors.forename: C
authors.initials: C
authors.lastname: Chen
authors.forename: T E
authors.initials: TE
authors.lastname: Spencer
authors.forename: F W
authors.initials: FW
authors.lastname: Bazer
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D004717:Endometrium / Q000737:chemistry / Q000378:metabolism; D004848:Epithelium / Q000502:physiology; D005260:Female; D051526:Fibroblast Growth Factor 10; D005346:Fibroblast Growth Factors / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D015870:Gene Expression; D006801:Humans; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D012756:Sheep; D017154:Stromal Cells / Q000502:physiology; D014599:Uterus / Q000737:chemistry / Q000502:physiology*
chemical_list: D018076:DNA, Complementary; C496463:FGF10 protein, human; D051526:Fibroblast Growth Factor 10; D012333:RNA, Messenger; D005346:Fibroblast Growth Factors