[Correlation between DNA topoisomerase II alpha expression and sensitivity to etoposide in human glioma cell lines].
The expression of topoisomerase II alpha (Topo II alpha) was investigated in six human glioma cell lines as a function of doubling time, cell cycle distribution, and sensitivity to an antineoplastic agent, etoposide (ETP). The Topo II alpha level was determined by immunohistochemical and flow cytometric studies using monoclonal antibody, 8D2, specific for Topo II alpha. In all cell lines, the Topo II alpha level correlated directly with sensitivity to ETP, but not with doubling time or percentage of cells in any phase of the cell cycle. These findings suggest that the cytocidal activity of ETP may be mediated by qualitative and quantitative changes in Topo II alpha in human glioma cells. We speculate that better knowledge of Topo II alpha expression in surgical specimens may lead to more individualized use of ETP in patients with malignant glioma.
Hao
Y J
YJ
Dept. of Neurosurgery, Nagoya University School of Medicine.
Natsume
A
A
Mizuno
M
M
Taniguchi
K
K
Wakabayashi
T
T
Yoshida
J
J
D000951:Antigens, Neoplasm; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D001932:Brain Neoplasms / Q000201:enzymology* / Q000473:pathology; D002453:Cell Cycle; D002455:Cell Division; D004250:DNA Topoisomerases, Type II / Q000378:metabolism*; D004268:DNA-Binding Proteins; D004354:Drug Screening Assays, Antitumor; D005047:Etoposide / Q000494:pharmacology*; D005434:Flow Cytometry; D005910:Glioma / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000951:Antigens, Neoplasm; D000972:Antineoplastic Agents, Phytogenic; D004268:DNA-Binding Proteins; D007527:Isoenzymes; D005047:Etoposide; D004250:DNA Topoisomerases, Type II