title: Detection of Toxoplasma gondii by polymerase chain reaction in sera of acutely infected mice.
abstract: The presence of Toxoplasma gondii DNA was detected in sera of acutely infected mice by polymerase chain reaction. Adult mice were inoculated intraperitoneally with 5 x 10(3) T. gondii RH strain tachyzoites. Five mice were killed every 3 hr from 3 to 21 hr post infection (PI) and every day from 1 to 7 days PI. Toxoplasma gondii DNA was first detected in 60% of the infected mice 18 hr PI and in 100% of the animals 21 hr PI and from 1 to 7 days PI. No mice survived longer than 7 days.
authors.affiliation: Groupe immunité des muqueuses et agents pathogènes, Faculté de médecine Jacques Lisfranc, Saint Etienne, France.
authors.forename: J
authors.initials: J
authors.lastname: Hafid
authors.forename: D
authors.initials: D
authors.lastname: Guichard
authors.forename: P
authors.initials: P
authors.lastname: Flori
authors.forename: T
authors.initials: T
authors.lastname: Bourlet
authors.forename: H
authors.initials: H
authors.lastname: Raberin
authors.forename: C
authors.initials: C
authors.lastname: Genin
authors.forename: R T
authors.initials: RT
authors.lastname: Sung
mesh_terms: D000208:Acute Disease; D000818:Animals; D016054:DNA, Protozoan / Q000097:blood*; D004587:Electrophoresis, Agar Gel; D005260:Female; D051379:Mice; D018512:Parasitemia / Q000175:diagnosis* / Q000469:parasitology; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D014122:Toxoplasma / Q000235:genetics / Q000302:isolation & purification*; D014124:Toxoplasmosis, Animal / Q000175:diagnosis* / Q000469:parasitology
chemical_list: D016054:DNA, Protozoan