title: Differential distribution of spicule matrix proteins in the sea urchin embryo skeleton.
abstract: Spicule matrix proteins are the products of primary mesenchyme cells, and are present in calcite spicules of the sea urchin embryo. To study their possible roles in skeletal morphogenesis, monoclonal antibodies against SM50, SM30 and another spicule matrix protein (29 kDa) were obtained. The distribution of these proteins in the embryo skeleton was observed by immunofluorescent staining. In addition, their distribution inside the spicules was examined by a 'spicule blot' procedure, direct immunoblotting of proteins embedded in crystallized spicules. Our observations showed that SM50 and 29 kDa proteins were enriched both outside and inside the triradiate spicules of the gastrulae, and also existed in the corresponding portions of growing spicules in later embryos and micromere cultures. The straight extensions of the triradiate spicules and thickened portions of body rods in pluteus spicules were also rich in these proteins. The SM30 protein was only faintly detected along the surface of spicules. By examination using the spicule blot procedure, however, SM30 was clearly detectable inside the body rods and postoral rods. These results indicate that SM50 and 29 kDa proteins are concentrated in radially growing portions of the spicules (normal to the c-axis of calcite), while SM30 protein is in the longitudinally growing portions (parallel to the c-axis). Such differential distribution suggests the involvement of these proteins in calcite growth during the formation of three-dimensionally branched spicules.
authors.affiliation: Department of Biology, Tokyo Metropolitan University, Hachioji, Japan. VYB04604@nifty.ne.jp
authors.forename: T
authors.initials: T
authors.lastname: Kitajima
authors.forename: H
authors.initials: H
authors.lastname: Urakami
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000954:Antigens, Surface / Q000378:metabolism; D002478:Cells, Cultured / Q000166:cytology / Q000201:enzymology; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D016326:Extracellular Matrix Proteins; D019084:Fluorescent Antibody Technique, Indirect; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008648:Mesoderm; D051379:Mice; D010980:Platelet Membrane Glycoproteins; D012617:Sea Urchins / Q000196:embryology* / Q000378:metabolism; D060149:Tetraspanin 30
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000954:Antigens, Surface; C554587:Cd63 protein, mouse; D003598:Cytoskeletal Proteins; D016326:Extracellular Matrix Proteins; D008562:Membrane Glycoproteins; D010980:Platelet Membrane Glycoproteins; C051691:SM50 protein, sea urchin; D060149:Tetraspanin 30; C071119:SM30 protein, Strongylocentrotus purpuratus