General effect of Sam68 on Rev/Rex regulated expression of complex retroviruses.
We have previously demonstrated that overexpression of Sam68 functionally substitutes for, as well as synergizes with, HIV-1 Rev in RRE-mediated gene expression and virus replication. In addition, C-terminal deletion mutants of Sam68 exhibit a transdominant negative phenotype in HIV replication. We now report that Sam68 also enhances the activities of Rev-like proteins of other complex retroviruses (e.g. HTLV-1 and EIAV) on their respective RNA targets. Furthermore, we demonstrate that Sam68 can function alone as well as synergize with Rev-MS2 and/or Rex-MS2 chimeric proteins on expression mediated by the corresponding RRE-MS2 fusion RNA element. Additionally, dominant negative mutants of Sam68 also repressed the synergistic activation of Sam68 with Rex, E-Rev, and/or Rev-MS2/Rex-MS2 on their corresponding RNA targets. Thus, Sam68 may play an important role in the post-transcriptional regulation of all complex retroviruses. Oncogene (2000) 19, 4071 - 4074
Reddy
T R
TR
Department of Medicine, University of California San Diego, La Jolla, California, CA 92093-0665, USA.
Xu
W D
WD
Wong-Staal
F
F
D000818:Animals; D001692:Biological Transport; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D015694:Gene Products, rev / Q000502:physiology*; D016370:Gene Products, rex / Q000502:physiology*; D005799:Genes, Dominant; D017930:Genes, Reporter; D015497:HIV-1 / Q000235:genetics / Q000502:physiology; D015368:Human T-lymphotropic virus 1 / Q000235:genetics / Q000502:physiology*; D004860:Infectious Anemia Virus, Equine / Q000235:genetics / Q000502:physiology*; D017909:Levivirus / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D016601:RNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000502:physiology; D015533:Transcriptional Activation; D014162:Transfection; D054321:rev Gene Products, Human Immunodeficiency Virus
D015694:Gene Products, rev; D016370:Gene Products, rex; D012367:RNA, Viral; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D054321:rev Gene Products, Human Immunodeficiency Virus; D015500:Chloramphenicol O-Acetyltransferase