title: Expression and secretion of scytalidopepsin B, an acid protease from Scytalidium lignicolum, in yeast.
abstract: An expression and secretion system for scytalidopepsin B, an acid protease from Scytalidium lignicolum, was constructed in yeast. Saccharomyces cerevisiae AH22 was transformed with an yeast-E. coli shuttle vector, pAM82, in which an yeast invertase signal segment and the cDNA encoding the pro- and mature enzyme regions were inserted. The transformant was found to secret a pepstatin-insensitive acid protease, when cultured aerobically in a low phosphate (Pi) medium. Amino terminal amino acid sequencing analysis indicated that the recombinant acid protease was accurately processed and secreted as a mature form.
authors.affiliation: Department of Applied Microbial Technology, sojo University, Kumamoto, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Shimuta
authors.forename: N
authors.initials: N
authors.lastname: Oda-Ueda
authors.forename: M
authors.initials: M
authors.lastname: Washio
authors.forename: H
authors.initials: H
authors.lastname: Oyama
authors.forename: K
authors.initials: K
authors.lastname: Oda
authors.forename: D
authors.initials: D
authors.lastname: Tsuru
mesh_terms: D000595:Amino Acid Sequence; D016282:Aspartic Acid Endopeptidases / Q000235:genetics*; D001483:Base Sequence; D004271:DNA, Fungal; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004792:Enzyme Precursors / Q000235:genetics*; D015870:Gene Expression; D003904:Mitosporic Fungi / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000378:metabolism
chemical_list: D004271:DNA, Fungal; D004792:Enzyme Precursors; D016282:Aspartic Acid Endopeptidases; C041236:Scytalidium lignicolum acid proteases