[Analysis of DNA excreted from the body as an approach to diagnosis and monitoring tumor growth].
Proceeding from their early data showing that some portion of DNA originating from apoptotic cells can enter the blood stream and pass through the renal barrier by preserving its template capabilities, the authors analyzed urine DNA from 29 patients with colorectal cancer. PCR was used to compare DNA samples from the normal mucosa surrounding the tumor and from the urine collected just prior to surgery. Six microsatellite loci were studied with oligonucleotide primers. The following results were obtained: i) 3 cases showed differences in one of the studied loci in normal and tissue DNA; ii) some patients displayed changes in urine DNA microsatellite loci, namely: disappearance of some alleles (loss of heterozygocity) and appearance of new ones; iii) there were no differences in microsatellite patterns of lymphocytic DNA (taken as a control) and urine DNA in healthy donors. The findings are discussed in view of current concepts of tumor clonal heterogeneity and interpreted as a promising approach to diagnosing and monitoring tumor growth.
Moliaka
Iu K
IuK
Botezatu
I V
IV
Potapova
G I
GI
Kosorukova
I S
IS
Serdiuk
O I
OI
Alekhina
R P
RP
Shelepov
V P
VP
Anan
V S
VS
Ogorodnikova
E V
EV
Melkonian
O S
OS
Umanskiĭ
S R
SR
Knysh
V I
VI
Likhtenshteĭn
A V
AV
D000230:Adenocarcinoma / Q000473:pathology / Q000652:urine*; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis; D015179:Colorectal Neoplasms / Q000473:pathology / Q000652:urine*; D017931:DNA Primers / Q000737:chemistry; D004273:DNA, Neoplasm / Q000652:urine*; D018450:Disease Progression; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D016482:Urinalysis
D014408:Biomarkers, Tumor; D017931:DNA Primers; D004273:DNA, Neoplasm