Antioxidant adaptive response of human mononuclear cells to UV-B: effect of lipoic acid.
Supplementation of human mononuclear cells with 3 and 6 mM of lipoic acid produces an inhibition of the antioxidant adaptive response triggered by treatment with UV-B light (0.30 W/m2 for 15 min). Supplementation with 1.5 mM of lipoic acid gives no conclusive results. The adaptive response is characterized by an increase in the activities of superoxide dismutase, catalase, glutathione peroxidase and DT-diaphorase. Catalase (5.5 +/- 0.6 pmol/mg prot) increases its activity by up to 22 +/- 3 pmol/mg prot, after irradiation with UV-B. Supplementation with 3 and 6 mM of lipoic acid completely inhibits the adaptive response. The activities of the membrane-bound mitochondrial enzymes succinate dehydrogenase and cytochrome oxidase do not increase after UV-B exposure. Moreover, their activities are found to decrease and the addition of lipoic acid does not prevent this effect. The inhibition of the antioxidant response by lipoic acid in human cells appears as indirect evidence of the existence of oxidative stress in the development of this response. As lipoic acid behaves as an effective antioxidant, it seems that its action decreases the intracellular oxidative signals necessary to develop the adaptive response in human mononuclear cells.
Alvarez
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S
Laboratory of Free Radical Biology, School of Pharmacy and Biochemistry, University of Buenos Aires, Argentina.
Boveris
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A
D000328:Adult; D002374:Catalase / Q000097:blood* / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D008058:Dihydrolipoamide Dehydrogenase / Q000097:blood / Q000528:radiation effects; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood* / Q000528:radiation effects; D006801:Humans; D066298:In Vitro Techniques; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000201:enzymology / Q000528:radiation effects*; D008297:Male; D013482:Superoxide Dismutase / Q000097:blood* / Q000528:radiation effects; D008063:Thioctic Acid / Q000494:pharmacology*; D014466:Ultraviolet Rays
D008063:Thioctic Acid; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D008058:Dihydrolipoamide Dehydrogenase