Allelic diversity of Mhc-DRB alleles in rhesus macaques.
The Biomedical Primate Research Centre (BPRC) rhesus macaque colony was started with a large number of wild-caught animals originating mainly from the Indian subcontinent. The contemporary self-sustaining colony comprises approximately 800 individuals. We screened a large section of the colony for Mamu-DRB polymorphisms by applying the denaturing gradient gel electrophoresis (DGGE) technique. Based on disparate DGGE profiles, animals were selected for nucleotide sequence analysis. This approach allowed the detection of 25 unreported Mamu-DRB alleles, bringing to 126 the total number of alleles documented in the literature. This communication demonstrates that rhesus macaques, like humans, display extensive allelic polymorphism at the DRB region. Phylogenetic analyses illustrate that humans and rhesus macaques share several Mhc-DRB loci and lineages. Identical exon 2 sequences, however, which are shared between humans and rhesus macaques, were not observed. This indicates that most primate Mhc-DRB alleles are of post-speciation origin.
Otting
N
N
Department of Immunobiology, Biomedical Primate Research Centre, Rijswijk, The Netherlands. otting@bprc.nl
de Groot
N G
NG
Noort
M C
MC
Doxiadis
G G
GG
Bontrop
R E
RE
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D004252:DNA Mutational Analysis; D004587:Electrophoresis, Agar Gel; D006684:HLA-DR Antigens / Q000235:genetics*; D006650:Histocompatibility Testing; D006801:Humans; D008253:Macaca mulatta / Q000235:genetics*; D008969:Molecular Sequence Data; D009691:Nucleic Acid Denaturation; D010802:Phylogeny; D011110:Polymorphism, Genetic
D006684:HLA-DR Antigens