Role of the outer membrane in the accumulation of quinolones by Serratia marcescens.
Accumulation of four quinolones by Serratia marcescens was measured fluorometrically. The passage of quinolones through the outer membrane was studied in both lipopolysaccharide-deficient and porin-deficient mutants. The lipopolysaccharide (LPS) layer formed a partially effective barrier for highly hydrophobic quinolones such as nalidixic acid. Quinolones with a low relative hydrophobicity coefficient seemed to pass preferentially through the water-filled Omp3 porin channels. Results were confirmed when Omp3 was cloned in a porin-defective Escherichia coli.
Berlanga
M
M
Laboratory of Microbiology, University of Barcelona, Spain.
Ruiz
N
N
Hernandez-Borrell
J
J
Montero
T
T
Viñas
M
M
D042462:4-Quinolones; D000890:Anti-Infective Agents / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005470:Fluorometry / Q000379:methods; D008070:Lipopolysaccharides / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D018272:Porins / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D012706:Serratia marcescens / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*
D042462:4-Quinolones; D000890:Anti-Infective Agents; D008070:Lipopolysaccharides; D018272:Porins; D011994:Recombinant Proteins