Interaction of cellular proteins with the 5' end of Norwalk virus genomic RNA.
The lack of a susceptible cell line and an animal model for Norwalk virus (NV) infection has prompted the development of alternative strategies to generate in vitro RNAs that approximate the authentic viral genome. This approach has allowed the study of viral RNA replication and gene expression. In this study, using mobility shift and cross-linking assays, we detected several cellular proteins from HeLa and CaCo-2 cell extracts that bind to, and form stable complexes with, the first 110 nucleotides of the 5' end of NV genomic RNA, a region previously predicted to form a double stem-loop structure. These proteins had molecular weights similar to those of the HeLa cellular proteins that bind to the internal ribosomal entry site of poliovirus RNA. HeLa proteins La, PCBP-2, and PTB, which are important for poliovirus translation, and hnRNP L, which is possibly implicated in hepatitis C virus translation, interact with NV RNA. These protein-RNA interactions are likely to play a role in NV translation and/or replication.
Gutiérrez-Escolano
A L
AL
Departamento de Patología Experimental, Centro de Investigación y de Estudios Avanzados del IPN, Mexico City, Mexico. alonso@mail.cinvestav.mx
Brito
Z U
ZU
del Angel
R M
RM
Jiang
X
X
D001324:Autoantigens / Q000378:metabolism*; D001483:Base Sequence; D018938:Caco-2 Cells; D002457:Cell Extracts; D017124:Conserved Sequence; D004268:DNA-Binding Proteins / Q000378:metabolism*; D006367:HeLa Cells; D034664:Heterogeneous-Nuclear Ribonucleoprotein L; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D006801:Humans; D009663:Norwalk virus / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D038941:Polypyrimidine Tract-Binding Protein; D011485:Protein Binding; D012367:RNA, Viral / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000378:metabolism*; D014157:Transcription Factors; D000097585:SS-B Antigen
D001324:Autoantigens; D002457:Cell Extracts; D004268:DNA-Binding Proteins; D034664:Heterogeneous-Nuclear Ribonucleoprotein L; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D038941:Polypyrimidine Tract-Binding Protein; D012367:RNA, Viral; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; D014157:Transcription Factors; D000097585:SS-B Antigen