Genetic analysis of the ycgJ-metB-cysK-ygaG operon negatively regulated by the VirR/VirS system in Clostridium perfringens.
The 5'-flanking region of the metB-cysK-ygaG operon, whose expression is negatively regulated by the VirR/VirS system in C. perfringens, was analyzed. The region contained the ycgJ, mscL, and colA genes encoding a hypothetical protein, a large conductance mechanosensitive channel protein, and kappa-toxin (collagenase), respectively. Northern analysis revealed that the ycgJ gene was transcribed as a 4.9-kb operon together with the metB-cysK-ygaG genes and that this operon was negatively regulated by the VirR/VirS system. It is indicated that the pfoA (theta-toxin or perfringolysin O), colA, and ycgJ-metB-cysK-ygaG genes that belong to the VirR/VirS regulon are situated very close together in a 26.5-kb region of the chromosome, but do not form a pathogenic island.
Ohtani
K
K
Department of Microbiology, Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki, Japan.
Takamura
H
H
Yaguchi
H
H
Hayashi
H
H
Shimizu
T
T
D001426:Bacterial Proteins / Q000235:genetics*; D019757:Carbon-Oxygen Lyases / Q000235:genetics*; D003016:Clostridium perfringens / Q000235:genetics*; D003547:Cysteine Synthase / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D009876:Operon; D012329:RNA, Bacterial; D012333:RNA, Messenger
D001426:Bacterial Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; C068267:mry protein, Streptococcus pyogenes; D003547:Cysteine Synthase; C022696:O-succinylhomoserine (thiol)-lyase; D019757:Carbon-Oxygen Lyases