title: Formation of hydrogen bonds precedes the rate-limiting formation of persistent structure in the folding of ACBP.
abstract: A burst phase in the early folding of the four-helix two-state folder protein acyl-coenzyme A binding protein (ACBP) has been detected using quenched-flow in combination with site-specific NMR-detected hydrogen exchange. Several of the burst phase structures coincide with a structure consisting of eight conserved hydrophobic residues at the interface between the two N and C-terminal helices. Previous mutation studies have shown that the formation of this structure is rate limiting for the final folding of ACBP. The burst phase structures observed in ACBP are different from the previously reported collapsed types of burst phase intermediates observed in the folding of other proteins.
authors.affiliation: Department of Protein Chemistry, Institute of Molecular Biology, University of Copenhagen, Oster Farimagsgade 2A, Copenhagen K, DK-1353, Denmark.
authors.forename: K
authors.initials: K
authors.lastname: Teilum
authors.forename: B B
authors.initials: BB
authors.lastname: Kragelund
authors.forename: J
authors.initials: J
authors.lastname: Knudsen
authors.forename: F M
authors.initials: FM
authors.lastname: Poulsen
mesh_terms: D000577:Amides / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism*; D002417:Cattle; D017124:Conserved Sequence; D026261:Diazepam Binding Inhibitor; D006859:Hydrogen / Q000378:metabolism; D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D007532:Isoleucine / Q000378:metabolism; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D017510:Protein Folding; D017433:Protein Structure, Secondary; D011522:Protons; D012694:Serine / Q000378:metabolism
chemical_list: D000577:Amides; D002352:Carrier Proteins; D026261:Diazepam Binding Inhibitor; D011522:Protons; D007532:Isoleucine; D012694:Serine; D006859:Hydrogen