title: Assembly of tight junctions during early vertebrate development.
abstract: Tight junction formation during development is critical for embryonic patterning and organization. We consider mechanisms of junction biogenesis in cleaving mouse and Xenopus eggs. Junction assembly follows the establishment of cell polarity at 8-cell (mouse) or 2-cell (Xenopus) stages, characterized by sequential membrane delivery of constituents, coordinated by embryonic (mouse) or maternal (Xenopus) expression programmes. Cadherin adhesion is permissive for tight junction construction only in the mouse. Occludin post-translational modification and membrane delivery, mediated by delayed ZO-1 alpha(+)isoform expression in the mouse, provides a mechanism for completion of tight junction biogenesis and sealing, regulating the timing of blastocoel cavitation.
authors.affiliation: Division of Cell Sciences, School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, UK. tpf@soton.ac.uk
authors.forename: T P
authors.initials: TP
authors.lastname: Fleming
authors.forename: T
authors.initials: T
authors.lastname: Papenbrock
authors.forename: I
authors.initials: I
authors.lastname: Fesenko
authors.forename: P
authors.initials: P
authors.lastname: Hausen
authors.forename: B
authors.initials: B
authors.lastname: Sheth
mesh_terms: D000818:Animals; D019521:Body Patterning; D002970:Cleavage Stage, Ovum / Q000502:physiology* / Q000648:ultrastructure; D005260:Female; D008565:Membrane Proteins / Q000502:physiology; D051379:Mice; D008954:Models, Biological; D010750:Phosphoproteins / Q000502:physiology; D011247:Pregnancy; D019108:Tight Junctions / Q000502:physiology* / Q000648:ultrastructure; D014981:Xenopus; D029867:Xenopus Proteins; D062826:Zonula Occludens-1 Protein
chemical_list: D008565:Membrane Proteins; D010750:Phosphoproteins; C569045:TJP1 protein, Xenopus; C569043:Tjp1 protein, mouse; D029867:Xenopus Proteins; D062826:Zonula Occludens-1 Protein