Fas ligand and Bax gene transcription contributes to Ro60 ribonucleoprotein redistribution in UV-A irradiated human keratinocytes.
OBJECTIVE
Ro ribonucleoproteins are of particular interest because they are serologic markers of photosensitive variants of lupus such as the subacute cutaneous lupus erythematosus (SCLE), in which the polycyclic skin lesions are triggered by exposure to the sun. We study the role of apoptosis in the expression of Ro antigen.


METHODS
We used UV-A irradiated keratinocytes.


RESULTS
We demonstrate in cultured human UVA-irradiated keratinocytes that the enhanced expression of Ro60 ribonucleoprotein is caused by antigenic redistribution consecutive to Fas-L and Bax gene activation.
Bollain-y-Goytia
J J
JJ
Department of Immunology, CBE, Universidad Autónoma de Zacatecas, Guadelupe, Mexico.
Avalos-Díaz
E
E
Herrera-Esparza
R
R
D017209:Apoptosis / Q000235:genetics*; D001324:Autoantigens / Q000378:metabolism* / Q000528:radiation effects; D015153:Blotting, Western; D002478:Cells, Cultured; D053222:Fas Ligand Protein; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007091:Image Processing, Computer-Assisted; D017403:In Situ Hybridization; D015603:Keratinocytes / Q000378:metabolism / Q000528:radiation effects*; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D019253:Proto-Oncogene Proteins c-bcl-2; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins / Q000378:metabolism* / Q000528:radiation effects; D014158:Transcription, Genetic; D014466:Ultraviolet Rays; D051028:bcl-2-Associated X Protein; D000099207:SS-A Antigen
D001324:Autoantigens; D053222:Fas Ligand Protein; D008562:Membrane Glycoproteins; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D051028:bcl-2-Associated X Protein; C494826:BAX protein, human; C505661:FASLG protein, human; C116006:RO60 protein, human; D000099207:SS-A Antigen