title: Human milk fat globule antigen 1 (HMFG1) expression in prostatic carcinoma and immunotargeting with a radiolabelled monoclonal anti-HMFG1 antibody.
abstract: BACKGROUND
  Prostatic cancer is the leading cause of death in Swedish men. Approximately 50% have disseminated disease at diagnosis. Radiolabelled antibodies could possibly be a treatment modality for disseminated prostatic cancer, so that in this study the expression of the human milk fat globulin 1 (HMFG1) antigen in prostate cancer was examined.
  
  
  METHODS
  An immunohistochemistry technique with a murine monoclonal antibody was used, as well as the human prostate cancer cell line DU-145, which expresses this cell surface antigen. TUR specimens from patients with prostate cancer were also examined.
  
  
  RESULTS
  Eighteen out of 22 (82%) patients exhibited an HMFG1-positive tumour. An inhomogenity in the immunostaining could occasionally be seen, with smaller apparently negative areas. The immunolocalisation properties of the antibody were investigated using a radiolabelled antibody injection into nude mice bearing heterotransplants of the DU-145 cell line. The highest accumulation of the antibody was seen in the tumour tissue and the liver.
  
  
  CONCLUSIONS
  The results obtained form a basis for further investigations with the goal of using the antibodies for staging and therapy for prostate cancer.
authors.affiliation: Department of Oncology, Sahlgrenska University Hospital, Göteborg, Sweden.
authors.forename: G
authors.initials: G
authors.lastname: Ljung
authors.forename: B
authors.initials: B
authors.lastname: Lennernäs
authors.forename: H
authors.initials: H
authors.lastname: Nordgren
authors.forename: A
authors.initials: A
authors.lastname: Holmberg
authors.forename: J E
authors.initials: JE
authors.lastname: Westlin
authors.forename: S
authors.initials: S
authors.lastname: Nilsson
authors.forename: M
authors.initials: M
authors.lastname: Edgren
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D006017:Glycolipids / Q000032:analysis* / Q000276:immunology; D006023:Glycoproteins / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D066292:Lipid Droplets; D008297:Male; D051379:Mice; D008819:Mice, Nude; D011471:Prostatic Neoplasms / Q000737:chemistry / Q000000981:diagnostic imaging*; D016719:Radioimmunodetection; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D006017:Glycolipids; D006023:Glycoproteins; C110367:milk fat globule