title: Enhancement in the perfection of orthorhombic lysozyme crystals grown in a high magnetic field (10 T).
abstract: Orthorhombic crystals of hen egg-white (HEW) lysozyme were grown in a homogeneous and static magnetic field of 10 T. All crystals grown at 10 T were oriented such that their crystallographic c axes were parallel to the magnetic field direction and showed a narrower average full-width at half-maximum (FWHM) of the rocking curve than those grown at 0 T. Rocking-width measurements were made at the BL-10A station at the Photon Factory, Tsukuba, Japan, using a high-resolution vertical-type four-circle diffractometer. Crystal perfection was evaluated using the FWHM of the rocking curve; the effects of the magnetic field on the quality of the crystals were examined by comparison of the FWHM of seven crystals grown at 10 and 0 T. The FWHMs of the reflections along the a, b and c axes decreased by 23.5, 35.3 and 27.8%, respectively, and those of other general reflections decreased by 17.4-42.2% in the crystals grown at high magnetic field. These results clearly showed that a magnetic field of 10 T improved the crystal perfection of the orthorhombic lysozyme crystals. As a result, the maximum resolution of X-ray diffraction increased from 1.3 A at 0 T to 1.13 A at 10 T. The magnetic field also affected the dimensions of the unit cell, increments being 0.2% for the a and c axes and 0.1% for the b axis, respectively. These facts suggest that the application of a high magnetic field during crystallization might result in remarkable enhancements in the diffraction power of protein crystals having magnetic anisotropy.
authors.affiliation: Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sato
authors.forename: Y
authors.initials: Y
authors.lastname: Yamada
authors.forename: S
authors.initials: S
authors.lastname: Saijo
authors.forename: T
authors.initials: T
authors.lastname: Hori
authors.forename: R
authors.initials: R
authors.lastname: Hirose
authors.forename: N
authors.initials: N
authors.lastname: Tanaka
authors.forename: G
authors.initials: G
authors.lastname: Sazaki
authors.forename: K
authors.initials: K
authors.lastname: Nakajima
authors.forename: N
authors.initials: N
authors.lastname: Igarashi
authors.forename: M
authors.initials: M
authors.lastname: Tanaka
authors.forename: Y
authors.initials: Y
authors.lastname: Matsuura
mesh_terms: D000818:Animals; D002645:Chickens; D003460:Crystallization; D018360:Crystallography, X-Ray; D005260:Female; D008280:Magnetics; D009113:Muramidase / Q000737:chemistry*
chemical_list: D009113:Muramidase