The synthetic peptide related to the central part of human interleukin-2 molecule accelerates growth and vascularization of sarcoma 180 in mice.
The synthetic peptide C-1-6 related to the central part of human interleukin 2 molecule (sequence 59-72; N- and C-modified) had been shown previously to inhibit cytotoxic activity of macrophages converting them to synthesis of growth factors. In this paper the effect of C-1-6 on growth of sarcoma 180 in mice was studied. C-1-6 significantly accelerated tumor growth having been injected into mice in dose 5 or 50 microg per animal since the 4th day after tumor cells transplantation. Supernatants of Mphi in vitro activated by C-1-6 (10 microg/ml) and injected into mice also accelerated significantly sarcoma mass diurnal increasing as compared to mice treated with supernatants of non-activated Mphi or activated with bacterial lipopolysaccharide. A single injection of C-1-6 into mice either at the day or at the next day of tumor cells inoculation increased significantly the number of vessels growing up to transplant, thus the forming of the vascular bed had preceded tumor volume enlargement.
Okulov
V B
VB
Laboratory of Oncoimmunology, N. N. Petrov Research Institute of Oncology, St. Petersburg, Russia.
Ushmorov
A G
AG
Voytenkov
B O
BO
Onoprienko
L V
LV
Mikhaleva
I I
II
Ivanov
V T
VT
D000818:Animals; D006801:Humans; D007376:Interleukin-2 / Q000737:chemistry* / Q000494:pharmacology; D008297:Male; D051379:Mice; D009368:Neoplasm Transplantation; D009389:Neovascularization, Pathologic; D010446:Peptide Fragments / Q000008:administration & dosage / Q000494:pharmacology*; D010529:Peritoneal Cavity; D012510:Sarcoma 180 / Q000098:blood supply* / Q000473:pathology*
D007376:Interleukin-2; D010446:Peptide Fragments