title: Dietary nucleotides increase the proportion of a TCR gammadelta+ subset of intraepithelial lymphocytes (IEL) and IL-7 production by intestinal epithelial cells (IEC); implications for modification of cellular and molecular cross-talk between IEL and IEC by dietary nucleotides.
abstract: We have investigated the effects of dietary nucleotides on intraepithelial lymphocytes (IEL) and intestinal epithelial cells (IEC) in weanling mice. The proportion of T-cell receptor (TCR) gammadelta+ IEL in BALB/c mice fed a diet supplemented with nucleotides (NT(+) diet) was significantly higher than that in mice fed the nucleotide-free diet, while the proportion of TCR alphabeta+ IEL in NT(+) diet-fed mice was significantly decreased. The change of the TCR alphabeta+/TCR gammadelta+ ratio was mainly observed in a CD8 alphaalpha+ subset of IEL. IEC from NT(+) diet-fed mice produced a higher level of IL-7, which is important in the development of TCR gammadelta+ IEL, than those from control diet-fed mice. The expression levels of IL-7 and IL-2 receptors on IEL were not different between the two dietary groups. Our findings suggest that the increased population of a TCR gammadelta+ IEL subset by feeding nucleotides may be caused by the increased production of IL-7 by IEC.
authors.affiliation: Nutrition Science Institute, Meiji Milk Products Co., Ltd., Odawara, Kanagawa, Japan. n-shinya@mx7.mesh.ne.jp
authors.forename: S
authors.initials: S
authors.lastname: Nagafuchi
authors.forename: M
authors.initials: M
authors.lastname: Totsuka
authors.forename: S
authors.initials: S
authors.lastname: Hachimura
authors.forename: M
authors.initials: M
authors.lastname: Goto
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
authors.forename: T
authors.initials: T
authors.lastname: Yajima
authors.forename: T
authors.initials: T
authors.lastname: Kuwata
authors.forename: S
authors.initials: S
authors.lastname: Kaminogawa
mesh_terms: D000818:Animals; D003568:Cytidine Monophosphate / Q000378:metabolism*; D019587:Dietary Supplements; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D006157:Guanosine Monophosphate / Q000378:metabolism*; D006801:Humans; D007291:Inosine Monophosphate / Q000378:metabolism*; D015851:Interleukin-7 / Q000096:biosynthesis*; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism; D018655:Lymphocyte Count; D051379:Mice; D008807:Mice, Inbred BALB C; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism*; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis; D020395:Receptors, Interleukin-7 / Q000096:biosynthesis; D016176:T-Lymphocyte Subsets / Q000145:classification / Q000166:cytology / Q000378:metabolism*; D014542:Uridine Monophosphate / Q000378:metabolism*
chemical_list: D015851:Interleukin-7; D016692:Receptors, Antigen, T-Cell, gamma-delta; D015375:Receptors, Interleukin-2; D020395:Receptors, Interleukin-7; D007291:Inosine Monophosphate; D006157:Guanosine Monophosphate; D014542:Uridine Monophosphate; D003568:Cytidine Monophosphate