A microfluidic cartridge to prepare spores for PCR analysis.
A prototype cartridge system is described that rapidly disrupts Bacillus spores by sonication, adds PCR reagent to the disrupted spores, and dispenses the mixture into a PCR tube. The total time to automatically process the spores in the cartridge and then detect the spore DNA by real-time PCR was 20 min.
Belgrader
P
P
Cepheid, Sunnyvale, CA 94089, USA.
Okuzumi
M
M
Pourahmadi
F
F
Borkholder
D A
DA
Northrup
M A
MA
D001407:Bacillus / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods; D013170:Spores / Q000302:isolation & purification