title: A rapid procedure for the isolation of plasmid DNA from environmental bacteria.
abstract: The INSTA-MINI-PREP method, a rapid protocol for plasmid DNA extraction, was originally developed to prepare plasmid DNA from 1 to 3 ml miniprep Escherichia coli cultures. Direct extraction of plasmid DNA is achieved by a two-phase solution which is separated by centrifugation in the presence of the INSTA-PREP gel barrier material. This method has been successfully tested on various environmental Salmonella strains, although it was not suitable for Pseudomonas aeruginosa and enterococci strains. The INSTA-MINI-PREP method is a new alternative procedure to screen plasmid contents of Salmonella and E. coli strains rapidly and easily.
authors.affiliation: Department of Biotechnology, Polytechnic University of Valencia, Spain. mferrus@btc.upv.es
authors.forename: M A
authors.initials: MA
authors.lastname: Ferrús
authors.forename: J L
authors.initials: JL
authors.lastname: Alonso
authors.forename: I
authors.initials: I
authors.lastname: Amorós
authors.forename: M
authors.initials: M
authors.lastname: Hernández
authors.forename: J
authors.initials: J
authors.lastname: Hernández
mesh_terms: D001431:Bacteriological Techniques; D002498:Centrifugation / Q000379:methods; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics; D005618:Fresh Water; D005782:Gels; D010957:Plasmids / Q000235:genetics*; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000302:isolation & purification; D012475:Salmonella / Q000235:genetics* / Q000302:isolation & purification; D014871:Water Microbiology; D014876:Water Pollution
chemical_list: D004269:DNA, Bacterial; D005782:Gels