Detection of 14-3-3 brain protein in the cerebrospinal fluid of patients with paraneoplastic neurological disorders.
The detection of 14-3-3 protein in cerebrospinal fluid by immunoblotting is useful for the diagnosis of Creutzfeldt-Jakob disease (CJD). We found 14-3-3 protein in 10 of 80 (12.5%) patients with paraneoplastic neurological disorders (PNDs), whose presenting symptoms may mimic those of CJD. In 47 of 48 CJD patients, the 14-3-3 protein was detected as a single band, and it was detected as a double band in 1 patient. The double-band pattern was observed in 9 of 10 14-3-3 protein-positive patients with PNDs. The 14-3-3 protein assay may be positive in PND patients, but the immunoblotting pattern distinguishes most PND samples from those of CJD.
Saiz
A
A
Service of Neurology and Institute d'Investigació Biomèdica August Pi i Sunyer, Barcelona, Spain.
Graus
F
F
Dalmau
J
J
Pifarré
A
A
Marin
C
C
Tolosa
E
E
D048948:14-3-3 Proteins; D000368:Aged; D001921:Brain / Q000378:metabolism*; D007562:Creutzfeldt-Jakob Syndrome / Q000134:cerebrospinal fluid / Q000175:diagnosis; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D015151:Immunoblotting; D020363:Limbic Encephalitis / Q000134:cerebrospinal fluid*; D008297:Male; D008875:Middle Aged; D020362:Paraneoplastic Cerebellar Degeneration / Q000134:cerebrospinal fluid*; D020361:Paraneoplastic Syndromes, Nervous System / Q000134:cerebrospinal fluid* / Q000175:diagnosis; D011237:Predictive Value of Tests; D011506:Proteins / Q000032:analysis*; D014446:Tyrosine 3-Monooxygenase
D048948:14-3-3 Proteins; D011506:Proteins; D014446:Tyrosine 3-Monooxygenase