New aqueous two-phase system based on cashew-nut tree gum and poly(ethylene glycol).
The characterisation of a new system based on cashew-nut tree gum, a branched acidic heteropolysaccharide found in Brazil, and poly(ethylene glycol) (PEG) was studied. Phase diagrams are provided for the PEG-cashew-nut tree gum system. The influence of PEG molecular mass, tie-line length and pH on bovine serum albumin (BSA) partition was investigated. Protein partition coefficient was little influenced by changing PEG molecular mass. Increasing the tie-line length decreased the partition. Increasing the pH also raised the BSA partition coefficient. It is shown that systems formed by PEG and cashew-nut tree gum may be considered as an interesting alternative for use in protein purification.
Sarubbo
L A
LA
DESQ-FEQ, UNICAMP, Campinas-SP-Brasil, Brazil.
Oliveira
L A
LA
Porto
A L
AL
Duarte
H S
HS
Carneiro-Leão
A M
AM
Lima-Filho
J L
JL
Campos-Takaki
G M
GM
Tambourgi
E B
EB
D002240:Carbohydrate Sequence; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011092:Polyethylene Glycols / Q000737:chemistry*; D012116:Resins, Plant / Q000737:chemistry*; D014197:Trees / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D012116:Resins, Plant; D014867:Water; D011092:Polyethylene Glycols