EGTA treatment causes the synthesis of heat shock proteins in sea urchin embryos.
Paracentrotus lividus embryos, at post-blastular stage, when subjected to a rise in temperature from physiologic (20 degrees C) to 31 degrees C, synthesize a large group of heat shock proteins (hsps), and show a severe inhibition of bulk protein synthesis. We show, by mono- and two-dimensional electrophoresis, that also EGTA (ethylene glycol-bis[beta-aminoethyl ether] tetraacetic acid) treatment induces in sea urchin embryos both marked inhibition of bulk protein synthesis and the synthesis of the entire set of hsps. Furthermore, EGTA-treated sea urchin embryos are able to survive at a temperature otherwise lethal (35 degrees C) becoming thermotolerant. Because incubation with a different calcium-chelator, EDTA (ethylenediaminetetraacetic acid), or in calcium-free medium did not induce hsps synthesis we conclude that the stress response caused by EGTA is not related to its calcium chelator function.
Roccheri
M C
MC
Dipartimento di Biologia Cellulare e dello Sviluppo "A. Monroy,", Università di Palermo, Viale delle Scienze, Palermo, 90128, Italy. mariroc@unipa.it
Onorato
K
K
Tipa
C
C
Casano
C
C
D000818:Animals; D002614:Chelating Agents / Q000494:pharmacology*; D004492:Edetic Acid / Q000378:metabolism; D004533:Egtazic Acid / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional; D004625:Embryo, Nonmammalian / Q000187:drug effects* / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000378:metabolism; D012617:Sea Urchins / Q000196:embryology* / Q000502:physiology
D002614:Chelating Agents; D006360:Heat-Shock Proteins; D004533:Egtazic Acid; D004492:Edetic Acid