MALDI-MS and HPLC quantification of oligosaccharides of lichenase-hydrolyzed water-soluble beta-glucan from ten barley varieties.
This study is the first to apply matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) to both qualitative and quantitative analyses of oligosaccharides of lichenase-hydrolyzed water-soluble beta-glucan from barley. Compared to high-performance liquid chromatography (HPLC) with an evaporative light-scattering detector, MALDI-MS is a rapid technique with high accuracy and sensitivity and could be used to assess primary structural features of water-soluble beta-glucan from different barley varieties.
Jiang
G
G
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Canada.
Vasanthan
T
T
D002851:Chromatography, High Pressure Liquid; D005936:Glucans / Q000737:chemistry*; D006026:Glycoside Hydrolases / Q000737:chemistry*; D001467:Hordeum / Q000737:chemistry*; D006868:Hydrolysis; D009844:Oligosaccharides / Q000032:analysis*; D012995:Solubility; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014867:Water / Q000737:chemistry
D005936:Glucans; D009844:Oligosaccharides; D014867:Water; D006026:Glycoside Hydrolases; C028020:licheninase