title: Membrane association of presynaptic cytomatrix protein bassoon.
abstract: Components of the specialized cytomatrix at active zones of presynaptic nerve terminals are thought to be involved in organizing synaptic events such as immobilisation or translocation of synaptic vesicles and assemblingactive zone components. The 420-kDa non-transmembraneprotein Bassoon is a specific componentof the presynaptic cytomatrix that shares features with both cytoskeleton-associated and peripheral-membrane proteins. Using immunogold electron microscopy we show here that synapse associated Bassoon is distributed in a subregion of active zones. Using a biochemical assay we show that a fraction of Bassoon is membrane associated. Electron microscopy performed on the same biochemical fraction further revealed that Bassoon is associated with vesicular structures. Together these data suggest that at least a fraction of Bassoon is associated with a membraneous compartment in neurons.
authors.affiliation: Department of Neurochemistry/Molecular Biology, Leibniz Institute for Neurobiology, Brenneckestr. 6, Magdeburg, D-39118, Germany.
authors.forename: L
authors.initials: L
authors.lastname: Sanmartí-Vila
authors.forename: S
authors.initials: S
authors.lastname: tom Dieck
authors.forename: K
authors.initials: K
authors.lastname: Richter
authors.forename: W
authors.initials: W
authors.lastname: Altrock
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: W
authors.initials: W
authors.lastname: Volknandt
authors.forename: H
authors.initials: H
authors.lastname: Zimmermann
authors.forename: C C
authors.initials: CC
authors.lastname: Garner
authors.forename: E D
authors.initials: ED
authors.lastname: Gundelfinger
authors.forename: T
authors.initials: T
authors.lastname: Dresbach
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002458:Cell Fractionation; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism* / Q000648:ultrastructure; D002499:Centrifugation, Density Gradient; D003599:Cytoskeleton / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D006624:Hippocampus / Q000166:cytology; D008565:Membrane Proteins / Q000378:metabolism*; D016253:Microscopy, Immunoelectron; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D017729:Presynaptic Terminals / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D016708:Synaptophysin / Q000378:metabolism
chemical_list: C113688:Bsn protein, rat; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D016708:Synaptophysin