Lactococcus lactis as a cell factory for high-level diacetyl production.
We report the engineering of Lactococcus lactis for the efficient conversion of sugar into diacetyl by combining NADH-oxidase overproduction and alpha-acetolactate decarboxylase inactivation. Eighty percent of the carbon flux was found to be rerouted via alpha-acetolactate to the production of diacetyl by preloading the cells with NADH-oxidase before their use as a cell factory.
Hugenholtz
J
J
Wageningen Centre for Food Sciences and NIZO Food Research, 6710 BA Ede, The Netherlands. hugenhol@nizo.nl
Kleerebezem
M
M
Starrenburg
M
M
Delcour
J
J
de Vos
W
W
Hols
P
P
D002262:Carboxy-Lyases / Q000037:antagonists & inhibitors; D003931:Diacetyl / Q000378:metabolism*; D005818:Genetic Engineering; D007773:Lactates / Q000378:metabolism; D013294:Lactococcus lactis / Q000235:genetics* / Q000378:metabolism; D009097:Multienzyme Complexes / Q000235:genetics / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics / Q000378:metabolism
D007773:Lactates; D009097:Multienzyme Complexes; C006359:alpha-acetolactate; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases; D002262:Carboxy-Lyases; C023746:acetolactate decarboxylase; D003931:Diacetyl