title: Natural genetic exchanges between vaccine and wild poliovirus strains in humans.
abstract: In a previous study of poliovirus vaccine-derived strains isolated from patients with vaccine-associated paralytic poliomyelitis (VAPP) (9, 11), we reported that a high proportion (over 50%) of viruses had a recombinant genome. Most were intertypic vaccine/vaccine recombinants. However, some had restriction fragment length polymorphism (RFLP) profiles different from those of poliovirus vaccine strains. We demonstrate here that five such recombinants, of 88 VAPP strains examined, carried sequences of wild (nonvaccine) origin. To identify the parental wild donor of these sequences, we used RFLP profiles and nucleotide sequencing to look for similarity in the 3D polymerase-coding region of 61 wild, cocirculating poliovirus isolates (43 type 1, 16 type 2, and 2 type 3 isolates). In only one case was the donor identified, and it was a wild type 1 poliovirus. For the other four vaccine/wild recombinants, the wild parent could not be identified. The possibility that the wild sequences were of a non-poliovirus-enterovirus origin could not be excluded. Another vaccine/wild recombinant, isolated in Belarus from a VAPP case, indicated that the poliovirus vaccine/wild recombination is not an isolated phenomenon. We also found wild polioviruses (2 of 15) carrying vaccine-derived sequences in the 3' moiety of their genome. All these results suggest that genetic exchanges with wild poliovirus and perhaps with nonpoliovirus enteroviruses, are also a natural means of evolution for poliovirus vaccine strains.
authors.affiliation: Molecular Epidemiology of Enteroviruses, Institut Pasteur, Paris, France.
authors.forename: S
authors.initials: S
authors.lastname: Guillot
authors.forename: V
authors.initials: V
authors.lastname: Caro
authors.forename: N
authors.initials: N
authors.lastname: Cuervo
authors.forename: E
authors.initials: E
authors.lastname: Korotkova
authors.forename: M
authors.initials: M
authors.lastname: Combiescu
authors.forename: A
authors.initials: A
authors.lastname: Persu
authors.forename: A
authors.initials: A
authors.lastname: Aubert-Combiescu
authors.forename: F
authors.initials: F
authors.lastname: Delpeyroux
authors.forename: R
authors.initials: R
authors.lastname: Crainic
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002648:Child; D002675:Child, Preschool; D002522:Chlorocebus aethiops; D004527:Egg Proteins; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D010243:Paralysis / Q000209:etiology / Q000821:virology; D011051:Poliomyelitis / Q000150:complications / Q000821:virology; D017955:Poliovirus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D011055:Poliovirus Vaccine, Oral / Q000009:adverse effects / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D012367:RNA, Viral / Q000032:analysis; D016865:Reassortant Viruses / Q000235:genetics*; D011956:Receptors, Cell Surface / Q000235:genetics; D011995:Recombination, Genetic; D016415:Sequence Alignment; D017423:Sequence Analysis, RNA; D014709:Vero Cells; D014774:Virulence
chemical_list: D004527:Egg Proteins; D011055:Poliovirus Vaccine, Oral; D012367:RNA, Viral; D011956:Receptors, Cell Surface; C030474:vitellogenin receptor