Determination of morphine 3-esters in rabbit plasma by high-performance liquid chromatography with ultraviolet detection.
A sensitive high-performance liquid chromatographic (HPLC) method for the quantitation of the morphine 3-esters 1[3-(2, 2-dimethylvaleroyl)-morphine (A), 3-(2-phenylbenzoyl)-morphine (B) and 3-(2,2-diphenylpropionyl)-morphine (C)] in rabbit plasma is described. Sample preparation was based on reversed-phase solid-phase extraction. The compounds were separated on C(18) reversed-phase analytical columns and then determined by ultraviolet detection. The recovery from plasma was 78.7 +/- 7.4%, 69.1 +/- 6.9% and 75 +/- 7.2% (mean +/- SD) for A, B, and C, respectively. The present method enabled the detection limit of 0.2, 0.2 and 0.1 ng and quantification limit of 20, 10 and 10 ng/ml for A, B and C, respectively. The developed method was used for determination of the plasmakinetics of these morphine 3-esters in rabbits.
Otter
K
K
Department of Pharmacology, Christian-Albrechts University, Hospitalstrasse 4, D-24105 Kiel, Germany.
Mignat
C
C
Heber
D
D
Ziegler
A
A
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004952:Esters; D008297:Male; D009020:Morphine / Q000097:blood* / Q000737:chemistry; D011817:Rabbits; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D004952:Esters; D009020:Morphine