Effects of hepatocyte growth factor anti-sense oligodeoxynucleotides or met D/D genotype on mouse molar crown morphogenesis.
Hepatocyte growth factor (HGF) is considered to be one of the mediators of epithelio-mesenchymal interactions during early organogenesis and to be also involved in the development of murine molars. In the developing tooth, HGF is expressed in the cells of the dental papillae, and c-Met, its receptor, in the cells of dental epithelia. In order to study the functional role played by HGF in tooth development, we tested the effects of HGF translation arrest by anti-sense phosphorothioate oligodeoxynucleotides on E-14 molars cultured in vitro. We also analyzed the histo-morphogenesis and crown cytodifferentiation of transgenic met E-14 molars cultured in vitro. 3D reconstructions revealed perturbations of the cusp pattern. However, histo-morphogenesis and crown cytodifferentiation were normal at the histological level.
Schmitt
R
R
INSERM U424, Institut de Biologie Médicale, Faculté de Médecine, Strasbourg, France.
Fausser
J L
JL
Lesot
H
H
Ruch
J V
JV
D000818:Animals; D005838:Genotype; D017228:Hepatocyte Growth Factor / Q000037:antagonists & inhibitors* / Q000235:genetics* / Q000502:physiology; D066298:In Vitro Techniques; D051379:Mice; D008822:Mice, Transgenic; D008963:Molar / Q000196:embryology*; D009805:Odontogenesis / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D020319:Oligodeoxyribonucleotides, Antisense / Q000235:genetics* / Q000494:pharmacology*; D019859:Proto-Oncogene Proteins c-met / Q000235:genetics* / Q000502:physiology; D019228:Tooth Crown / Q000196:embryology
D020319:Oligodeoxyribonucleotides, Antisense; D017228:Hepatocyte Growth Factor; D019859:Proto-Oncogene Proteins c-met