Screening of wound-responsive genes identifies an immediate-early expressed gene encoding a highly charged protein in mechanically wounded tobacco plants.
In order to identify genes that are temporally and spatially regulated during wound response, a cDNA population in mechanically wounded tobacco leaves was screened by the fluorescence differential display method. Of 28 clones initially identified to have altered levels of transcripts within 3 h of wounding, eight were characterized. Although each clone showed a unique pattern of transcript accumulation, one distinct clone was further characterized because of its immediate-early response. Its transcripts began to accumulate 10 min after wounding, reached a maximum level within 1 h and disappeared after 2 h. The response, which occurred repeatably and systemically, was observed by the treatment with propionic acid or erythrosin B, indicating that cytosolic acidification could be one of the signals for immediate-early response of this gene. The cDNA encodes a polypeptide of 513 amino acids with a relative molecular mass of 60,952. The putative polypeptide is rich in lysine (K), glutamic acid (E) and aspartic acid (D), which constitute up to 70% of total amino acids, and was therefore designated as KED. The KED polypeptide is composed of a highly hydrophilic N-terminal region and a relatively hydrophobic C-terminal region, suggesting that KED may function through electrostatic interactions with cellular components.
Hara
K
K
Research and Education Center for Genetic Information, Nara Institute of Science and Technology, Ikoma, Japan.
Yagi
M
M
Koizumi
N
N
Kusano
T
T
Sano
H
H
D000595:Amino Acid Sequence; D003513:Cycloheximide / Q000494:pharmacology; D003600:Cytosol / Q000502:physiology; D018076:DNA, Complementary; D004533:Egtazic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D004923:Erythrosine / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017781:Genes, Immediate-Early; D017874:Immediate-Early Proteins / Q000737:chemistry / Q000235:genetics*; D007700:Kinetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D019319:Okadaic Acid / Q000494:pharmacology; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D010947:Plants, Toxic; D011422:Propionates / Q000494:pharmacology; D017386:Sequence Homology, Amino Acid; D019311:Staurosporine / Q000494:pharmacology; D013997:Time Factors; D014026:Nicotiana / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic
D018076:DNA, Complementary; D017874:Immediate-Early Proteins; D010940:Plant Proteins; D011422:Propionates; D019319:Okadaic Acid; D004533:Egtazic Acid; D003513:Cycloheximide; D019311:Staurosporine; C029658:propionic acid; C025603:1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid; D004923:Erythrosine