title: Successful large volume leukapheresis on a small infant allogeneic donor.
abstract: We successfully performed a hematopoietic stem cell apheresis on the smallest allogeneic donor reported to date, a 6.1 kg female. After placement of a dialysis catheter in the left femoral vein, the COBE Spectra was primed with one unit of paternal whole blood. Heparin and anticoagulant citrate dextrose, solution A (ACD-A) were slowly administered to the patient. Ionized calcium levels were checked hourly and calcium gluconate was given for hypocalcemia. Coagulation parameters were checked throughout the procedure. We collected 4.4 x 10(6) CD34+ cells/kg (recipient). The donor tolerated the procedure well and was discharged the following day. Five months later, the child manifests no obvious late effects.
authors.affiliation: Pediatric Blood and Marrow Transplant Program, Department of Pediatrics, Rainbow Babies and Children's Hospital and Case Western Reserve University School of Medicine, Cleveland, Ohio, USA.
authors.forename: J
authors.initials: J
authors.lastname: Pahys
authors.forename: V
authors.initials: V
authors.lastname: Fisher
authors.forename: M
authors.initials: M
authors.lastname: Carneval
authors.forename: R
authors.initials: R
authors.lastname: Yomtovian
authors.forename: R
authors.initials: R
authors.lastname: Sarode
authors.forename: M
authors.initials: M
authors.lastname: Nieder
mesh_terms: D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007223:Infant; D007937:Leukapheresis / Q000379:methods*; D014019:Tissue Donors