Cardiac troponin I and cardiac troponin T increases in pigs during ischemia-reperfusion damage.
In this study we addressed the question of whether the measurement of cardiac Troponin T (cTnT) and cardiac Troponin I (cTnI) is able to detect myocardial cell damage in an ischemia-reperfusion model in pigs. To answer the question 3 pigs were anaesthesized and a cardiac arrest was induced by electric fibrillation. After 5 minutes of global ischemia the cardiac arrest was reversed by electric defibrillation until normal perfusion was restored. We could clearly demonstrate an increase of cTnT and cTnI 30 minutes after reperfusion indicating myocardial injury during ischemia and subsequent reperfusion. The cTnT as well as the cTnI serum levels increased till 180 minutes after reperfusion. This ischemia-reperfusion injury is likely induced by oxygen radicals generated during hypoxia and subsequent reperfusion We conclude from our first results that troponin measurements with commercial available test kits may also reflect myocardial cell damage in pigs as it was recently demonstrated in rats. Further studies are needed for correlation of troponin serum levels and histopathological damage in this model especially if it is used to test beneficial or toxicological effects of radical neutralizing drugs.
Bertsch
T
T
Institute for Clinical Chemistry, Faculty of Clinical Medicine Mannheim of the University of Heidelberg, Mannheim, Germany. thomas.bertsch@ikc.ma.uni-heidelberg.de
Janke
C
C
Denz
C
C
Weiss
M
M
Luiz
T
T
Ellinger
K
K
Korth
U
U
Hannak
D
D
Bartelt
U
U
Krieter
H
H
D000818:Animals; D016887:Cardiopulmonary Resuscitation; D004554:Electric Countershock; D005609:Free Radicals; D006861:Hydrogen Peroxide / Q000378:metabolism; D007118:Immunoassay; D015428:Myocardial Reperfusion Injury / Q000097:blood*; D009206:Myocardium / Q000737:chemistry; D013552:Swine; D019210:Troponin I / Q000097:blood*; D020107:Troponin T / Q000097:blood*
D005609:Free Radicals; D019210:Troponin I; D020107:Troponin T; D006861:Hydrogen Peroxide