title: Comparison of separation behavior of benzodiazepines in packed capillary electrochromatography and open-tubular capillary electrochromatography.
abstract: Packed column capillary electrochromatography (CEC), open-tubular CEC and microcolum liquid chromatography (LC) using a cholesteryl silica bonded phase have been studied to compare the retention behavior for benzodiazepines. It has been found that packed column CEC gives better resolution, faster analysis time than microcolumn LC for benzodiazepines maintaining similar selectivity except for some solutes which are charged species under the separation conditions. However, open-tubular CEC gave different selectivities to a larger extent for charged benzodiazepines from that which should be produced by the chromatographic properties of the cholesteryl silica phase. Charged species migration times are mainly influenced by electrophoretic mobility rather than the chromatographic interactions.
authors.affiliation: School of Materials Science, Toyohashi University of Technology, Japan. jinno@chrom.tutms.tut.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Jinno
authors.forename: H
authors.initials: H
authors.lastname: Sawada
authors.forename: A P
authors.initials: AP
authors.lastname: Catabay
authors.forename: H
authors.initials: H
authors.lastname: Watanabe
authors.forename: N B
authors.initials: NB
authors.lastname: Sabli
authors.forename: J J
authors.initials: JJ
authors.lastname: Pesek
authors.forename: M T
authors.initials: MT
authors.lastname: Matyska
mesh_terms: D001569:Benzodiazepines / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000379:methods; D019075:Electrophoresis, Capillary / Q000379:methods*; D013997:Time Factors
chemical_list: D001569:Benzodiazepines