In situ imaging of detergent-resistant membranes by atomic force microscopy.
Purified detergent-resistant membranes (DRMs) are powerful tools for the biochemical study of plasma membrane domains. To what extent these isolated DRMs correspond to native membrane domains remains, however, a matter of debate. The most immediate question to be answered concerns the in situ size range of DRMs, a determination that escapes classical microscopy techniques. In this study we show that in situ three-dimensional images of a material as fragile as Triton X-100-treated cells can be obtained, in buffer, by tapping mode atomic force microscopy. These images establish that, prior to the isolation procedure, the detergent plasma membrane fragments form domains whose size frequently exceeds 15-20 microm(2). This DRMs size range is about 1 order of magnitude higher than that estimated for the larger microdomains of living cells, which strongly suggests that membrane microdomains rearrange into larger DRMs during Triton X-100 treatment. Concomitantly, the images also reveal the presence of the cytoskeleton, which is resistant to detergent extraction, and suggest that, in situ, DRMs are associated with the membrane cytoskeleton.
Giocondi
M C
MC
Centre de Biochimie Structurale, INSERM U414, 29 rue de Navacelles, Montpellier Cedex, France.
Vié
V
V
Lesniewska
E
E
Goudonnet
J P
JP
Le Grimellec
C
C
D000818:Animals; D002021:Buffers; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure*; D002522:Chlorocebus aethiops; D003080:Cold Temperature; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D003902:Detergents / Q000494:pharmacology*; D005557:Formaldehyde; D018625:Microscopy, Atomic Force; D017830:Octoxynol / Q000494:pharmacology*; D010316:Particle Size; D011108:Polymers; D012995:Solubility / Q000187:drug effects
D002021:Buffers; D003902:Detergents; D011108:Polymers; D005557:Formaldehyde; D017830:Octoxynol; C003043:paraform