A review of fluorescence in situ hybridization (FISH): current status and future prospects.
Fluorescence in situ hybridization (FISH) is a powerful technique for detecting DNA or RNA sequences in cells, tissues and tumors. This molecular cytogenetic technique enables the localization of specific DNA sequences within interphase chromatin and metaphase chromosomes and the identification of both structural and numerical chromosome changes. FISH is quickly becoming one of the most extensively used cytochemical staining techniques owing to its sensitivity and versatility, and with the improvement of current technology and cost effectiveness, its use will surely continue to expand. Here we review the wide variety of current applications and future prospects of FISH technology.
Nath
J
J
Genetics and Developmental Biology Program, West Virginia University, Morgantown 26506-6108, USA. jnath@wvu.edu
Johnson
K L
KL
D000818:Animals; D002467:Cell Nucleus / Q000737:chemistry / Q000235:genetics; D002869:Chromosome Aberrations / Q000235:genetics; D020732:Cytogenetic Analysis; D004273:DNA, Neoplasm / Q000235:genetics; D019143:Evolution, Molecular; D005554:Forensic Medicine / Q000379:methods; D015894:Genome, Human; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods* / Q000639:trends*; D020161:Physical Chromosome Mapping
D004273:DNA, Neoplasm