Detection of Toxoplasma gondii by polymerase chain reaction in sera of acutely infected mice.
The presence of Toxoplasma gondii DNA was detected in sera of acutely infected mice by polymerase chain reaction. Adult mice were inoculated intraperitoneally with 5 x 10(3) T. gondii RH strain tachyzoites. Five mice were killed every 3 hr from 3 to 21 hr post infection (PI) and every day from 1 to 7 days PI. Toxoplasma gondii DNA was first detected in 60% of the infected mice 18 hr PI and in 100% of the animals 21 hr PI and from 1 to 7 days PI. No mice survived longer than 7 days.
Hafid
J
J
Groupe immunité des muqueuses et agents pathogènes, Faculté de médecine Jacques Lisfranc, Saint Etienne, France.
Guichard
D
D
Flori
P
P
Bourlet
T
T
Raberin
H
H
Genin
C
C
Sung
R T
RT
D000208:Acute Disease; D000818:Animals; D016054:DNA, Protozoan / Q000097:blood*; D004587:Electrophoresis, Agar Gel; D005260:Female; D051379:Mice; D018512:Parasitemia / Q000175:diagnosis* / Q000469:parasitology; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D014122:Toxoplasma / Q000235:genetics / Q000302:isolation & purification*; D014124:Toxoplasmosis, Animal / Q000175:diagnosis* / Q000469:parasitology
D016054:DNA, Protozoan