Inhibitory effect of mast cell-mediated immediate-type allergic reactions by sulfapyridine.
We examined the effect of sulfapyridine on mast cell-mediated immediate-type allergic reactions. Sulfapyridine (1 and 10 microg/kg) significantly inhibited systemic allergic reaction induced by compound 48/80 in rats. Sulfapyridine (1 and 10 microg/kg) also inhibited significantly local mast cell-mediated immediate-type allergic reactions activated by anti-dinitrophenyl (DNP) IgE. Moreover, sulfapyridine inhibited histamine release dose-dependently in the rat peritoneal mast cells (RPMC) activated by compound 48/80 or anti-DNP IgE. When sulfapyridine was added, the level of cAMP in RPMC, transiently and significantly increased about 4-fold compared with that of basal cells. These results indicate that sulfapyridine inhibits mast cell-mediated immediate-type allergic reactions in vivo and in vitro.
Kim
H M
HM
College of Pharmacy, Professional Graduate School of Oriental Medicine, Center of Oriental Medicinal Science, Wonkwang University, Iksan, Chonbuk, South Korea. hmkim@wonnms.wonkwang.ac.kr
An
N H
NH
Yi
B H
BH
Chae
H J
HJ
Kim
H R
HR
Moon
S J
SJ
Kim
J J
JJ
Park
S T
ST
Baek
S H
SH
D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D004140:Dinitrophenols / Q000276:immunology; D006636:Histamine Release / Q000187:drug effects; D006969:Hypersensitivity, Immediate / Q000517:prevention & control*; D007111:Immunity, Cellular / Q000187:drug effects; D066298:In Vitro Techniques; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects; D010529:Peritoneal Cavity / Q000166:cytology; D051381:Rats; D017208:Rats, Wistar; D012709:Serum Albumin / Q000276:immunology; D013427:Sulfapyridine / Q000494:pharmacology*; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D004140:Dinitrophenols; D012709:Serum Albumin; C058064:dinitrophenyl-human serum albumin conjugate; D003189:p-Methoxy-N-methylphenethylamine; D000242:Cyclic AMP; D013427:Sulfapyridine