Novel natural products from soil DNA libraries in a streptomycete host.
As a route to accessing the potential chemical diversity of uncultivable microbes from the soil, combinatorial biosynthetic libraries were constructed by cloning large fragments of DNA isolated from soil into a Streptomyces lividans host. Four novel compounds, terragines A (1), B (2), C (3), and D (4), were isolated from recombinant 436-s4-5b1, and another novel compound, terragine E (5), was isolated from 446-s3-102g1. The structures were determined by a combination of spectroscopic techniques, primarily 2D NMR.
Wang
G Y
GY
TerraGen Discovery Inc., 300-2386 East Mall-UBC, Vancouver, B.C., Canada V6T 1Z3.
Graziani
E
E
Waters
B
B
Pan
W
W
Li
X
X
McDermott
J
J
Meurer
G
G
Saxena
G
G
Andersen
R J
RJ
Davies
J
J
D000577:Amides / Q000737:chemistry / Q000378:metabolism*; D001688:Biological Products / Q000737:chemistry* / Q000235:genetics*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics*; D015723:Gene Library; D015394:Molecular Structure; D012988:Soil Microbiology; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism
D000577:Amides; D001688:Biological Products; D004269:DNA, Bacterial