Evidence of the existence of a new circulating recombinant form of HIV type 1 subtype A/J in Cameroon. The European Network on the Study of In Utero Transmission of HIV-1.
Several genetic subtypes and circulating recombinant forms (CRFs) of HIV-1 have been identified. The greatest degree of genetic diversity is displayed by variants from Central and West Africa. HIV-1 env C2-V5 and protease sequences were obtained from 15 HIV-1-infected pregnant women, who were selected from a larger cohort study in Yaoundé, Cameroon. Fourteen of 15 virus variants were shown to be recombinant, whereas a single variant appeared to be nonrecombinant subtype A. Five viruses were subtype A/J recombinants, with env genes derived from subtype A and protease genes derived from subtype J. Seven viruses clustered with reference sequences for CRF02 AG(IbNG) in both the env and protease gene fragments, and were thus subtype A/G recombinants. Two variants displayed even more complex recombination patterns. Phylogenetic analyses indicated that the five subtype A/J recombinants might be the first representatives of a previously unrecognized CRF.
Tscherning-Casper
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Microbiology and Tumor Biology Center, Karolinska Institute, Stockholm, Sweden. charlotte.tscherning@mtc.ki.se
Dolcini
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Mauclère
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Fenyö
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Barré-Sinoussi
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Albert
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Menu
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D002163:Cameroon / Q000453:epidemiology; D004279:DNA, Viral / Q000097:blood; D005260:Female; D015686:Gene Products, env / Q000235:genetics; D014644:Genetic Variation; D015658:HIV Infections / Q000453:epidemiology / Q000821:virology*; D016333:HIV Protease / Q000235:genetics; D015497:HIV-1 / Q000145:classification* / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000821:virology*; D011995:Recombination, Genetic / Q000235:genetics*; D017422:Sequence Analysis, DNA
D004279:DNA, Viral; D015686:Gene Products, env; D016333:HIV Protease