title: Serum level of soluble tumour necrosis factor receptor II (75 kDa) indicates inflammatory activity of sarcoidosis.
abstract: OBJECTIVE
  Tumour necrosis factor alpha (TNFalpha) is a key cytokine involved in granuloma formation of sarcoidosis. Since soluble TNF receptors (sTNF-R) are known to inhibit TNF effects, we were interested in whether they are elevated in the serum of sarcoidosis patients.
  
  
  METHODS
  We determined serum levels of sTNF-R I (55 kDa) and sTNF-R II (75 kDa) in 49 patients with sarcoidosis and 22 controls. The clinical course of the disease was re-evaluated in a follow-up after (mean +/- SE) 6.8 +/- 6.6 months.
  
  
  RESULTS
  sTNF-R I (3.1 +/- 1.1 ng mL-1, P < 0.05) and sTNF-R II (5.5 +/- 2.7 ng mL-1, P < 0.0005) were significantly elevated in sarcoidosis compared with controls (2.4 +/- 0.7 and 3.0 +/- 1.3 ng mL-1, respectively). Interestingly, both sTNF receptors were significantly higher in the serum of patients with active compared with inactive sarcoidosis (P < 0.005 and P < 0.0005, respectively). Furthermore, serum sTNF-R II levels were significantly higher in sarcoidosis patients with advanced radiological types II and III. In 10 patients, serum sTNF-R levels were obtained before and after systemic corticosteroid therapy and we observed a significant decrease of sTNF-R II (P < 0.02), whereas sTNF-R I levels were not reduced significantly.
  
  
  CONCLUSIONS
  Both types of sTNF receptors are elevated in the serum of sarcoidosis patients with active disease, but only the sTNF-R II seems to be useful for monitoring the inflammatory activity of the disease.
authors.affiliation: Research Centre Borstel, Medical Hospital, Borstel, Germany. zeigenhagen@fz-borstel.de
authors.forename: M W
authors.initials: MW
authors.lastname: Ziegenhagen
authors.forename: J
authors.initials: J
authors.lastname: Fitschen
authors.forename: N
authors.initials: N
authors.lastname: Martinet
authors.forename: M
authors.initials: M
authors.lastname: Schlaak
authors.forename: J
authors.initials: J
authors.lastname: Müller-Quernheim
mesh_terms: D000305:Adrenal Cortex Hormones / Q000627:therapeutic use; D000328:Adult; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007249:Inflammation / Q000097:blood / Q000276:immunology; D008297:Male; D008970:Molecular Weight; D011859:Radiography; D018124:Receptors, Tumor Necrosis Factor / Q000097:blood*; D012507:Sarcoidosis / Q000097:blood* / Q000000981:diagnostic imaging / Q000188:drug therapy / Q000276:immunology*
chemical_list: D000305:Adrenal Cortex Hormones; D018124:Receptors, Tumor Necrosis Factor