title: Tau pathology in diffuse neurofibrillary tangles with calcification (DNTC): biochemical and immunohistochemical investigation.
abstract: Diffuse neurofibrillary tangles with calcification (DNTC) represents a primary and sporadic presenile dementia that is characterized by temporal or fronto-temporal atrophy with diffuse neurofibrillary tangles (NFTs), neuropil threads and Fahr-type calcification without senile plaques. We examined the tau pathology in five autopsy cases of DNTC by immunoblotting and immunohistochemistry using phosphorylation-dependent and -independent anti-tau antibodies. The pattern of staining for different epitopes of beta-amyloid (A beta) was also investigated. NFTs were immunopositive with all the anti-tau antibodies used in this study. On the immunoblots, sarkosyl-insoluble tau appeared as three major bands of 60, 64 and 68 kDa, and as a minor band at 72 kDa. The majority of extracellular NFTs were weakly immunopositive only with the antibody recognizing the 40 carboxyl-terminal of A beta in DNTC. These results suggest that Alzheimer's disease-like tau pathology could exist independently of A beta deposits in DNTC.
authors.affiliation: Department of Neuropsychiatry, Okayama University Medical School, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Tanabe
authors.forename: H
authors.initials: H
authors.lastname: Ishizu
authors.forename: K
authors.initials: K
authors.lastname: Ishiguro
authors.forename: N
authors.initials: N
authors.lastname: Itoh
authors.forename: S
authors.initials: S
authors.lastname: Terada
authors.forename: T
authors.initials: T
authors.lastname: Haraguchi
authors.forename: K
authors.initials: K
authors.lastname: Kawai
authors.forename: S
authors.initials: S
authors.lastname: Kuroda
mesh_terms: D000368:Aged; D000544:Alzheimer Disease / Q000378:metabolism* / Q000473:pathology* / Q000503:physiopathology; D016229:Amyloid beta-Peptides / Q000276:immunology / Q000378:metabolism; D001284:Atrophy / Q000473:pathology / Q000503:physiopathology; D001921:Brain / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D002114:Calcinosis / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D000939:Epitopes / Q000276:immunology / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009410:Nerve Degeneration / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D016874:Neurofibrillary Tangles / Q000378:metabolism* / Q000473:pathology* / Q000648:ultrastructure; D010766:Phosphorylation; D016875:tau Proteins / Q000276:immunology / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D016229:Amyloid beta-Peptides; D000939:Epitopes; D016875:tau Proteins