[Preparation, evaluation of specificity and use of anti-Le monoclonal antibodies].
Anti-Le(a) and anti-Le(b) monoclonal antibodies were obtained and attempts at evaluating their epitope specificity have been made. A method for identification of Lewis phenotypes in salivary specimens by dot-I enzyme immunoassay has been developed. Analyses of salivary samples from 72 donors detected donors with phenotypes Le(a-b-) and Le(a+b+), which was confirmed by hemagglutination test.
Lapenkov
M I
MI
Nikolaeva
T L
TL
Aborina
E A
EA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007124:Immunoenzyme Techniques; D007983:Lewis Blood Group Antigens / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010641:Phenotype; D012463:Saliva / Q000737:chemistry / Q000276:immunology*; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D007983:Lewis Blood Group Antigens