Changes induced by oxygen in rat liver proteins identified by high-resolution two-dimensional gel electrophoresis.
Molecular oxygen (O2) regulates the expression of a variety of genes. Several of the proteins that respond to changes in oxygen concentration have been identified in a variety of cell lines. We extend these previous studies by analyzing the effect of oxygen on the entire protein expression profile of an intact organ using high-resolution two-dimensional gel electrophoresis. To this end, we used an isolated, in vitro perfused organ preparation to produce two groups of rat livers perfused with high (95% O2, 5% CO2) or low (95% N2, 5% CO2) oxygen concentrations. Using two-dimensional gel electrophoresis we compared the protein expression profiles of both groups of livers. Computer analysis of the files obtained after laser densitometry of the two-dimensional gels revealed two spots that were strongly up-regulated in high PO2 perfused livers compared with low PO2 perfused livers. These spots were analyzed by peptide mass fingerprinting analysis. These spots were identified as arginase 1 (liver-type arginase; EC 3.5.3.1) and mitochondrial enoyl-CoA hydratase 1 (EC 4.2.1.17). The possible role of these proteins in its new context of oxygen availability is discussed.
Miralles
C
C
Servei de Pneumologia, Unidad de Investigación, Hospital Univeritari Son Dureta, Palma, Mallorca, Spain.
Agustí
A G
AG
Aubry
C
C
Sanchez
J C
JC
Walzer
C
C
Hochstrasser
D
D
Busquets
X
X
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D008099:Liver / Q000378:metabolism*; D008297:Male; D010100:Oxygen / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D011506:Proteins; D010100:Oxygen