Factors influencing the biosorption of gadolinium by micro-organisms and its mobilisation from sand.
The present work was devoted to the study of the biosorption capacities of various microbial species (Bacillus subtilis, Pseudomonas aeruginosa, Ralstonia metallidurans CH34 previously Alcaligenes eutrophus CH34, Mycobacterium smegmatis, Saccharomyces cerevisiae) for ions of the lanthanide gadolinium (Gd3+). The uptake by sand of this element was also measured. Saturation curves and Scatchard models were established for all biosorbants used in this work. The results enabled us to determine the binding affinities and the maximum capacities for biosorption of Gd3+, which ranged from 350 micromol g(-1) for B. subtilis to 5.1 micromol g(-1) for S. cerevisiae. This study demonstrated the usefulness of optimisation of experimental conditions in biosorption investigations. Experimental results showed that biosorption could be influenced by the growth stage and by the composition of the growth medium of microbial cells. Finally, particular attention was given to the transfer of gadolinium ions from a loaded sand to a bacterial suspension.
Andrès
Y
Y
SUBATECH UMR 6457, Université de Nantes, Ecole des Mines, IN2P3/CNRS, France. andres@subatech.in2p3.fr
Thouand
G
G
Boualam
M
M
Mergeay
M
M
D000327:Adsorption; D001412:Bacillus subtilis / Q000254:growth & development / Q000378:metabolism; D001419:Bacteria / Q000254:growth & development / Q000378:metabolism*; D001665:Binding Sites; D003470:Culture Media; D020118:Cupriavidus necator / Q000254:growth & development / Q000378:metabolism; D005682:Gadolinium / Q000378:metabolism*; D007700:Kinetics; D020102:Mycobacterium smegmatis / Q000254:growth & development / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*; D012822:Silicon Dioxide
D003470:Culture Media; D012822:Silicon Dioxide; D005682:Gadolinium