HLA-DQB1 and HLA-DPB1 genotypes in severe preeclampsia.
OBJECTIVE
To clarify whether there are susceptible HLA-DQB1 or HLA-DPB1 alleles in women with preeclampsia.


METHODS
The frequency of HLA-DQB1 and HLA-DPB1 alleles was analyzed in 47 women with previous severe preeclampsia and compared with that in 85 normal fertile women. The types of HLA-DQB1 and HLA-DPB1 alleles were assessed using a polymerase chain reaction-restriction fragment length polymorphism method.


RESULTS
The frequency of HLA-DQB1*04 allele in previously preeclamptic women was 21.3% (20 of 94 loci) and in the controls was 11.2% (19 of 170 loci). Thus, the HLA-DQB1*04 allele frequency was significantly higher in preeclamptic women compared with controls (P <.05 by chi(2) test, OR 2.15, 95% confidence interval 1.08, 4.27). The frequency of other HLA-DQB1 alleles and all HLA-DPB1 alleles was not significantly different between groups. The incidence of homozygosity of HLA-DQB1 or HLA-DPB1 alleles in preeclamptic women was not significantly different compared with that of normal fertile women.


CONCLUSIONS
These data suggest that women who have the HLA-DQB1*04 allele might be susceptible to preeclampsia.
Honda
K
K
Department of Obstetrics and Gynecology, Niigata University School of Medicine, Japan.
Takakuwa
K
K
Hataya
I
I
Yasuda
M
M
Kurabayashi
T
T
Tanaka
K
K
D000328:Adult; D000483:Alleles; D002874:Chromosome Mapping; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006682:HLA-DP Antigens / Q000235:genetics*; D059868:HLA-DP beta-Chains; D006683:HLA-DQ Antigens / Q000235:genetics*; D059866:HLA-DQ beta-Chains; D006801:Humans; D007231:Infant, Newborn; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011225:Pre-Eclampsia / Q000235:genetics*; D011247:Pregnancy
D006682:HLA-DP Antigens; D059868:HLA-DP beta-Chains; C071170:HLA-DPB1 antigen; D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen