title: Characterization of gene expression of NsERFs, transcription factors of basic PR genes from Nicotiana sylvestris.
abstract: Three genes of NsERFs (EREBPs), transcription factors for GCC box of basic PR genes, were isolated from Nicotiana sylvestris. Analyses of transgenic tobacco carrying the NsERF promoter::GUS genes showed that expression of all NsERF genes in leaves was induced by ethylene. Sequence analyses indicated that the 5'-upstream region of NsERF3 gene has the GCC box. In contrast, the promoter regions of NsERF2 and 4 have no GCC box, whereas NsERF2 has a putative EIN3 binding site. Tissue/cell specific expression is also discussed.
authors.affiliation: Radioisotope Research Center, Kyoto University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kitajima
authors.forename: T
authors.initials: T
authors.lastname: Koyama
authors.forename: M
authors.initials: M
authors.lastname: Ohme-Takagi
authors.forename: H
authors.initials: H
authors.lastname: Shinshi
authors.forename: F
authors.initials: F
authors.lastname: Sato
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005966:Glucuronidase / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; C053376:pathogenesis-related proteins, plant; D005966:Glucuronidase