Expression and cellular distribution of perchloric acid-soluble protein is dependent on the cell-proliferating states of NRK-52E cells.
To clarify the biological role of kidney perchloric acid-soluble protein 1 (K-PSP1), its expression and intracellular distribution were examined in normal rat kidney epithelial NRK-52E cells. K-PSP1 expression was low during the proliferating phase and high in the stationary phase, and shown to have a negative relationship with the protein-synthesizing activity of the cells. Immunocytochemical studies revealed that K-PSP1 is predominantly located in the cytosol, especially in endoplasmic reticulum and Golgi apparatus of proliferating cells. In the stationary phase, K-PSP1 was not detected immunologically even though protein and mRNA expression were high. This disappearance of reactivity with anti-serum seems to be due to a conformational change in K-PSP1 induced by unknown factors. These results suggest that the role of K-PSP1 is to regulate cell proliferation, and this may be related to a previously reported ability to inhibit protein synthesis.
Kanouchi
H
H
Department of Bioscience and Biotechnology, Graduate School of Kyushu University, Fukuoka, Japan.
Oka
T
T
Asagi
K
K
Tachibana
H
H
Yamada
K
K
D000818:Animals; D002452:Cell Count; D002453:Cell Cycle; D002455:Cell Division; D002460:Cell Line; D003593:Cytoplasm / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D006056:Golgi Apparatus / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D007668:Kidney / Q000166:cytology* / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D011487:Protein Conformation; D011500:Protein Synthesis Inhibitors / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012260:Ribonucleases
D006360:Heat-Shock Proteins; D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; C097211:Rida protein, rat; D012260:Ribonucleases; D007930:Leucine