title: Decreased monosaccharide transport in renal brush-border membrane vesicles of spontaneously hypertensive rats.
abstract: Na(+)-dependent D-glucose and D-galactose transport were studied in brush-border membrane vesicles (BBMVs) from kidney cortex isolated from both spontaneously hypertensive rats (SHR) and their normotensive genetic control Wistar-Kyoto (WKY) rats. Initial rates and accumulation ratios of Na(+)-dependent D-glucose and D-galactose transport were significantly lower in SHR compared with WKY, the observed decreases being similar for both substrates. To explain the reduction in sugar transport by renal BBMVs, the density of Na(+)-dependent sugar cotransporters was studied in BBMVs from kidney cortex isolated from SHR and WKY rats. Phlorizin-specific binding and Western blot analysis indicated a reduction in the density of the cotransporters in SHR relative to WKY rats. This reduction was similar to those found for the initial rates and accumulation ratios for D-glucose and D-galactose in SHR. Na+ uptake, studied using 22Na+, was significantly increased in SHR, so the observed reduction in sugar transport could be due to disruption of the Na+ gradient between renal BBMVs in SHR. Furthermore, a significant decrease in the activity of Na(+)-K(+)-ATPase was observed in SHR. In conclusion, changes in the density of the Na(+)-dependent sugar cotransporter and in the Na+ gradient across the brush-border membranes might be involved in the observed reduction in sugar transport by renal BBMVs from SHR.
authors.affiliation: Departamento de Fisiología y Biología Animal, Facultad de Farmacia, Universidad de Sevilla, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Mate
authors.forename: M A
authors.initials: MA
authors.lastname: de la Hermosa
authors.forename: A
authors.initials: A
authors.lastname: Barfull
authors.forename: I
authors.initials: I
authors.lastname: Sánchez-Aguayo
authors.forename: J M
authors.initials: JM
authors.lastname: Planas
authors.forename: C M
authors.initials: CM
authors.lastname: Vázquez
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D001794:Blood Pressure; D001835:Body Weight; D002135:Calcium-Binding Proteins; D004305:Dose-Response Relationship, Drug; D005690:Galactose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006973:Hypertension / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D007672:Kidney Cortex / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D008871:Microvilli / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D009004:Monosaccharide Transport Proteins / Q000378:metabolism; D009005:Monosaccharides / Q000378:metabolism*; D009994:Osmolar Concentration; D033902:Periplasmic Binding Proteins; D010695:Phlorhizin / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D012964:Sodium / Q000378:metabolism / Q000494:pharmacology; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D014617:Vacuoles / Q000187:drug effects / Q000378:metabolism*
chemical_list: D002135:Calcium-Binding Proteins; D009004:Monosaccharide Transport Proteins; D009005:Monosaccharides; D033902:Periplasmic Binding Proteins; C023550:galactose-binding protein; D012964:Sodium; D010695:Phlorhizin; D000254:Sodium-Potassium-Exchanging ATPase; D005947:Glucose; D005690:Galactose