title: The effectiveness of monoenergetic neutrons at 565 keV in producing dicentric chromosomes in human lymphocytes at low doses.
abstract: The induction of dicentric chromosomes in human lymphocytes from one individual irradiated in vitro with monoenergetic neutrons at 565 keV was examined to provide additional data for an improved evaluation of neutrons with respect to radiation risk in radioprotection. The resulting linear dose-response relationship obtained (0.813 +/- 0.052 dicentrics per cell per gray) over the dose range of 0.0213-0.167 Gy is consistent with published results obtained for irradiation with neutrons from different sources and with different spectra at energies lower than 1000 keV. Comparing this value to previously published "average" dose-response curves obtained by different laboratories for (60)Co gamma rays and orthovoltage X rays resulted in maximum RBEs (RBE(m)) of about 37 +/- 8 and 16 +/- 4, respectively. However, when our neutron data were matched to low-LET dose responses that were constructed several years earlier for lymphocytes from the same individual, higher values of RBE(m) resulted: 76.0 +/- 29.5 for (60)Co gamma rays and 54.2 +/- 18.4 for (137)Cs gamma rays; differentially filtered 220 kV X rays produced values of RBE(m) between 20.3 +/- 2.0 or 37.0 +/- 7. 1. The results highlight the dependence of RBE(m) on the choice of low-LET reference radiation and raise the possibility that differential individual response to low-LET radiations may need to be examined more fully in this context.
authors.affiliation: Institute of Radiobiology, GSF-National Research Center for Environment and Health, Neuherberg, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Schmid
authors.forename: D
authors.initials: D
authors.lastname: Regulla
authors.forename: S
authors.initials: S
authors.lastname: Guldbakke
authors.forename: D
authors.initials: D
authors.lastname: Schlegel
authors.forename: M
authors.initials: M
authors.lastname: Bauchinger
mesh_terms: D000328:Adult; D002869:Chromosome Aberrations; D002877:Chromosomes, Human / Q000528:radiation effects* / Q000648:ultrastructure; D003037:Cobalt Radioisotopes / Q000009:adverse effects; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays / Q000009:adverse effects; D006801:Humans; D018499:Linear Energy Transfer; D008214:Lymphocytes / Q000528:radiation effects* / Q000648:ultrastructure; D008297:Male; D009502:Neutrons / Q000009:adverse effects*; D012062:Relative Biological Effectiveness
chemical_list: D003037:Cobalt Radioisotopes