Contents of sulfur amino acids, and cystathionine beta-synthase and gamma-lyase activities in various tissues from agkistroden blomhoffi (mamushi).
The concentrations of sulfur-containing amino acids, taurine, cystathionine, methionine and cystine, as well as cystathionine beta-synthase and gamma-lyase activities in various tissues of Agkistrodon blomhoffi (mamushi) were measured. The concentration of taurine in examined tissues was greater than the concentration of other sulfur-containing amino acids. The concentration of cystathionine in various tissues was also much higher than those of methionine and cystine, but the concentration of cystathionine in the brain was lower than that of methionine. In all tissues examined in this study, cystathionine beta-synthase activity was much higher than that of cystathionine gamma-lyase. The ratios of cystathionine beta-synthase to gamma-lyase activities in various tissues were 5.6 to approximately 85.6. The concentration of sulfur-containing amino acids in muscle and skin divided into eight portions of the body were also determined. The concentrations of methionine and cystine in each portion of muscle and skin were almost the same, but the concentrations of taurine and cystathionine in each portion of the body were varied.
Nakayama
K
K
Department of Chemistry, Kochi Medical School, Kohasu, Oko-cho, Nankoku-shi, Kochi 783-8505, Japan.
Awata
S
S
Zhang
J
J
Ebinuma
H
H
Mariyama
T
T
Kodama
H
H
D017836:Agkistrodon / Q000378:metabolism*; D000603:Amino Acids, Sulfur; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D003541:Cystathionine beta-Synthase / Q000378:metabolism*; D003542:Cystathionine gamma-Lyase / Q000378:metabolism*; D004789:Enzyme Activation; D009928:Organ Specificity; D014018:Tissue Distribution
D000603:Amino Acids, Sulfur; D003541:Cystathionine beta-Synthase; D003542:Cystathionine gamma-Lyase