A neutralizing antibody Fab-influenza haemagglutinin complex with an unprecedented 2:1 stoichiometry: characterization and crystallization.
The haemagglutinin HA is a trimer of identical subunits and is the more abundant viral surface glycoprotein of the influenza virus. It is the target of antibodies that neutralize viral infectivity. Antibodies that bind to HA with 3:1 and 1:1 stoichiometries have been identified. Here, an antibody whose Fab binds to HA with an unprecedented 2:1 Fab:HA stoichiometry is characterized. The complex has been crystallized and synchrotron data to 3.5 A resolution have been collected. Molecular replacement confirms the stoichiometry of the complex.
Gigant
B
B
Laboratoire d'Enzymologie et Biochimie Structurales, UPR 9063, Bâtiment 34, CNRS, 1 Avenue de la Terrasse, 91198 Gif sur Yvette CEDEX, France.
Barbey-Martin
C
C
Bizebard
T
T
Fleury
D
D
Daniels
R
R
Skehel
J J
JJ
Knossow
M
M
D000914:Antibodies, Viral / Q000737:chemistry*; D000936:Antigen-Antibody Complex / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry*; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry*; D009500:Neutralization Tests
D000914:Antibodies, Viral; D000936:Antigen-Antibody Complex; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007140:Immunoglobulin Fab Fragments