title: Probenecid interferes with renal oxidative metabolism: a potential pitfall in its use as an inhibitor of drug transport.
abstract: The anionic drug probenecid has been traditionally used as an inhibitor of renal organic anion transport. More recently the drug was found to inhibit organic cation transport as well, and it is used to retain intracellularly loaded fluorophores. In these investigations it is implicitly assumed that probenecid performs its activity through competition for transport. Here we studied the possibility that probenecid provokes its effect through inhibition of cellular oxidative metabolism. Oxygen consumption was measured in isolated rat kidney cortex mitochondria. At concentrations of 1 mM or higher, probenecid increased the resting state (state 4) and decreased the ADP-stimulated respiration (state 3). A complete loss in respiratory control was observed at 10 mM probenecid. After incubating isolated rat kidney proximal tubular cells (PTC) for 30 min with probenecid a concentration-dependent reduction in ATP content was observed, which was significant at concentrations of 1 mM and higher. Using digital image fluorescence microscopy the membrane potential in PTC was measured with bisoxonol. The mitochondrial effects of probenecid were paralleled by a depolarization of the plasma membrane, immediately after drug addition. All events are likely to be a result of membrane disordering due to the lipophilic character of probenecid, and may explain, at least in part, the various inhibitory effects found for the drug. We recommend to be cautious with applying probenecid in cellular research.
authors.affiliation: Department of Pharmacology and Toxicology 233 University Medical Centre Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands. R.Masereeuw@farm.kun.nl
authors.forename: R
authors.initials: R
authors.lastname: Masereeuw
authors.forename: A P
authors.initials: AP
authors.lastname: van Pelt
authors.forename: S H
authors.initials: SH
authors.lastname: van Os
authors.forename: P H
authors.initials: PH
authors.lastname: Willems
authors.forename: P
authors.initials: P
authors.lastname: Smits
authors.forename: F G
authors.initials: FG
authors.lastname: Russel
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis / Q000378:metabolism; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010101:Oxygen Consumption / Q000187:drug effects; D011339:Probenecid / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D014528:Uricosuric Agents / Q000494:pharmacology*
chemical_list: D014528:Uricosuric Agents; D000255:Adenosine Triphosphate; D011339:Probenecid; D002118:Calcium