title: Intrapair resemblance in very low calorie diet-induced weight loss in female obese identical twins.
abstract: OBJECTIVE
  To assess intrapair resemblance in changes of body weight, total body fat, fat distribution, resting metabolic rate, fasting respiratory quotient and cardiovascular disease risk factors in response to therapeutic weight loss in female obese identical twins.
  
  
  METHODS
  Patients stayed for 40 days on an inpatient metabolic unit under careful supervision. The stay was divided into three parts: an initial period of 7 days for adjustment to the hospital environment and for baseline measurements, 28 days of the weight reduction regimen when negative energy balance was achieved mainly by a very low calorie diet (1.6 MJ per day) and 5 days of testing after weight reduction.
  
  
  METHODS
  Fourteen pairs of premenopausal female obese identical twins (age: 39.0+/-1.7 y; body weight (BW): 93.9+/-21.2 kg; body mass index (BMI): 34.2+/-7.8 kg/m2) participated in the study.
  
  
  METHODS
  Before and after weight loss, the following measurements were made: body composition by anthropometry and hydrodensitometry, intra-abdominal fat by ultrasonography, resting metabolic rate by indirect calorimetry. Total cholesterol, high-density lipoprotein-cholesterol, triglycerides and uric acid were determined by standard laboratory procedures. Blood pressure was measured in the morning in the recumbent position.
  
  
  RESULTS
  Subjects lost 8.8+/-1.9 kg of weight, from 93.9+/-21.2 to 85.1+/-10.9 kg (P<0.0001) and 6.5+/-2.3 kg of body fat (P<0.001). Weight losses varied widely among subjects, with a high correlation between losses of members of twin pairs for body weight (r=0.85; P<0.001) and for body fat (r=0.88; P<0.0001). Changes in uric acid resulting from weight loss were also correlated among members of twin pairs whereas changes in blood pressure, cholesterol and triglycerides were not.
  
  
  CONCLUSIONS
  The great intrapair resemblance observed in very low calorie diet-induced weight and fat losses in female obese identical twins suggests an important role of genetic factors in response to the weight reduction regimen.
authors.affiliation: Obesity Unit, 3rd Department of Internal Medicine, The First Medical Faculty, Charles University, 128 08 Prague, Czech Republic. vahai@lf1.cuni.cz
authors.forename: V
authors.initials: V
authors.lastname: Hainer
authors.forename: A J
authors.initials: AJ
authors.lastname: Stunkard
authors.forename: M
authors.initials: M
authors.lastname: Kunesová
authors.forename: J
authors.initials: J
authors.lastname: Parízková
authors.forename: V
authors.initials: V
authors.lastname: Stich
authors.forename: D B
authors.initials: DB
authors.lastname: Allison
mesh_terms: D000273:Adipose Tissue / Q000000981:diagnostic imaging; D000328:Adult; D001823:Body Composition / Q000235:genetics*; D001835:Body Weight; D002318:Cardiovascular Diseases / Q000235:genetics; D002784:Cholesterol / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D004038:Diet, Reducing; D005260:Female; D006801:Humans; D008875:Middle Aged; D009765:Obesity / Q000178:diet therapy* / Q000235:genetics* / Q000378:metabolism; D012307:Risk Factors; D014280:Triglycerides / Q000097:blood; D014463:Ultrasonography; D014527:Uric Acid / Q000097:blood; D015431:Weight Loss / Q000235:genetics*
chemical_list: D008076:Cholesterol, HDL; D014280:Triglycerides; D014527:Uric Acid; D002784:Cholesterol