title: Cholinergic nerve fibres associated with the microvessels of the human cerebral cortex: a study based on monoclonal immunocytochemistry for choline acetyltrasferase.
abstract: The distribution of cholinergic nerve fibres associated with the microvasculature of the human parietal cerebral cortex was investigated by immunocytochemistry, employing monoclonal antibodies against choline acetyl-transferase, the acetylcholine-synthesizing enzyme. The results revealed strongly immunoreactive nerve fibres in the tunica adventitia of arterioles penetrating the superficial cortical layers from the pial vasculature. Networks of stained nerve fibres were seen within the tunica muscularis of the radially directed arterioles that cross the intermediate and deep cortical laminae, and of their transverse and recurrent branches. Tiny positive nerve fibres were also seen around the cortex capillaries, some reaching the endothelial cells. The morphological data support the involvement of acetylcholine in microvasculature local regulation, possibly with a differentiated role in the arterioles and capillaries.
authors.affiliation: Istituto di Anatomia Umana Normale, Facoltà di Medicina e Chirurgia, Università degli Studi di Bari, Policlinico, Italy. benagiano@anatomia.uniba.it
authors.forename: V
authors.initials: V
authors.lastname: Benagiano
authors.forename: D
authors.initials: D
authors.lastname: Virgintino
authors.forename: A
authors.initials: A
authors.lastname: Rizzi
authors.forename: M
authors.initials: M
authors.lastname: Errede
authors.forename: M
authors.initials: M
authors.lastname: Bertossi
authors.forename: V
authors.initials: V
authors.lastname: Troccoli
authors.forename: L
authors.initials: L
authors.lastname: Roncali
authors.forename: G
authors.initials: G
authors.lastname: Ambrosi
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D001160:Arterioles / Q000294:innervation* / Q000473:pathology; D002196:Capillaries / Q000294:innervation; D002536:Cerebral Arteries / Q000294:innervation* / Q000473:pathology; D002795:Choline O-Acetyltransferase / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D009412:Nerve Fibers / Q000201:enzymology* / Q000473:pathology; D010296:Parietal Lobe / Q000098:blood supply* / Q000473:pathology
chemical_list: D000911:Antibodies, Monoclonal; D002795:Choline O-Acetyltransferase