title: Therapy of acanthamoeba keratitis--an experimental study.
abstract: The study was conducted to determine the efficacy of various drugs/agents in the treatment of experimentally induced Acanthamoeba Keratitis (A. keratitis). Thirty rabbits were divided into 5 equal treatment groups and polyhexamethylene biguanide (PHMB) 0.02%, fluconazole 0.2%, povidone iodine 5%, aprotinin 40 IU/ml and normal saline were administered in a double blind manner, at a dose of one drop, 5 times/day. Neomycin 1700 U/ml was used as a common drug in all groups, with same frequency. Post therapy subsidence of lid oedema, mucopurulent discharge, conjunctival congestion, corneal epithelial defect and anterior chamber reactions were noted. Average healing time in days (Mean +/- S.D.) were 13.67 +/- 1.21, 22 +/- 1.41, 23.5 +/- 1.05, 17.0 +/- 1.41 and 28.0 +/- 1.90 for PHMB, fluconazole, povidone iodine, aprotinin and normal saline respectively. Lid oedema, mucopurulent discharge and conjunctival congestion improved faster in PHMB and aprotinin treated eyes. The results of this study suggest that PHMB is a better drug for A. keratitis, while aprotinin may be used as an adjuvant drug in the management of A. keratitis.
authors.affiliation: Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, Ansari Nagar, New Delhi.
authors.forename: A
authors.initials: A
authors.lastname: Panda
authors.forename: G K
authors.initials: GK
authors.lastname: Das
authors.forename: N R
authors.initials: NR
authors.lastname: Biswas
authors.forename: D
authors.initials: D
authors.lastname: Mittal
authors.forename: S
authors.initials: S
authors.lastname: Mohanty
mesh_terms: D015823:Acanthamoeba Keratitis / Q000188:drug therapy*; D000818:Animals; D001645:Biguanides / Q000627:therapeutic use; D005260:Female; D015725:Fluconazole / Q000627:therapeutic use; D008297:Male; D009355:Neomycin / Q000627:therapeutic use; D011206:Povidone-Iodine / Q000627:therapeutic use; D011817:Rabbits
chemical_list: D001645:Biguanides; C031233:polihexanide; D011206:Povidone-Iodine; D015725:Fluconazole; D009355:Neomycin