title: Characterization of CD1e, a third type of CD1 molecule expressed in dendritic cells.
abstract: Dendritic cells express several alternatively spliced CD1e mRNAs. These molecules encode proteins characterized by the presence of either one, two, or three alpha domains and either a 51- or 63-amino acid cytoplasmic domain. Moreover, mRNAs encoding isoforms lacking the transmembrane domain are observed. Several of these CD1e isoforms were expressed in transfected cells, and two of them, with three alpha domains, displayed a particular processing pathway. These latter isoforms slowly leave the endoplasmic reticulum due to the presence of atypical dilysine motifs in the cytoplasmic tail. These molecules are associated with the beta(2)-microglobulin and accumulate in late Golgi and late endosomal compartments. In the latter compartments, they are cleaved into soluble forms that appear to be stable. In dendritic cells, these isoforms are mainly located in the Golgi apparatus, and upon maturation they are redistributed to late endosomal compartments. This work demonstrates the existence of CD1e molecules. As compared with other CD1 molecules, CD1e displays fundamentally different properties and therefore may represent a third type of CD1 molecules.
authors.affiliation: Equipe Propre INSERM 99-08 and Unité INSERM 311, Etablissement Français du Sang-Alsace, Strasbourg 67065, France and UMR CNRS 144, Institut Curie, Paris 75005, France.
authors.forename: C
authors.initials: C
authors.lastname: Angenieux
authors.forename: J
authors.initials: J
authors.lastname: Salamero
authors.forename: D
authors.initials: D
authors.lastname: Fricker
authors.forename: J P
authors.initials: JP
authors.lastname: Cazenave
authors.forename: B
authors.initials: B
authors.lastname: Goud
authors.forename: D
authors.initials: D
authors.lastname: Hanau
authors.forename: H
authors.initials: H
authors.lastname: de La Salle
mesh_terms: D017398:Alternative Splicing; D018949:Antigens, CD1 / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D017931:DNA Primers; D003713:Dendritic Cells / Q000276:immunology*; D011992:Endosomes / Q000276:immunology; D006056:Golgi Apparatus / Q000276:immunology; D008969:Molecular Sequence Data; D012322:RNA Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology
chemical_list: D018949:Antigens, CD1; C417738:CD1e antigen; D017931:DNA Primers; D012322:RNA Precursors; D012333:RNA, Messenger; D011994:Recombinant Proteins