title: Detection of Epstein-Barr virus and human herpesvirus 7 and 8 genomes in primary cutaneous T- and B-cell lymphomas.
abstract: BACKGROUND
  Several studies have investigated the possible involvement of viral agents, particularly herpesviruses, in primary cutaneous lymphoma (PCL).
  
  
  OBJECTIVE
  Our aim was to screen for the presence of human herpesvirus 7 (HHV-7) and 8 (HHV-8) genomes in samples of PCL, and to determine if their presence was independent of Epstein-Barr virus (EBV).
  
  
  METHODS
  Screening was performed using polymerase chain reaction assay in 64 skin samples from historical lesional tissues with PCL.
  
  
  RESULTS
  Only nine cases showed positivity for HHV-7: four of 29 mycosis fungoides (MF), two of four CD30-positive large-cell cutaneous T-cell lymphoma (CTCL), two of 12 follicle centre cutaneous B-cell lymphoma (CBCL) and one of nine marginal zone CBCL. Fifteen cases tested positive for EBV: seven of 29 MF, two of four pleomorphic small/medium sized CTCL, three of three angiocentric CTCL, one of 12 follicle centre CBCL and two of nine marginal zone CBCL. All cases were uniformly negative for HHV-8. No simultaneous positivity was found for EBV and HHV-7. Controls tested negative for all viruses.
  
  
  CONCLUSIONS
  The findings indicate that EBV, HHV-7 and HHV-8 seem not to be involved in the pathogenesis of PCL.
authors.affiliation: Department of Dermatology, Hospital General Universitario, C/Denia 20-6(a), 46006 Valencia, Spain. eduyame@meditex.es
authors.forename: E
authors.initials: E
authors.lastname: Nagore
authors.forename: E
authors.initials: E
authors.lastname: Ledesma
authors.forename: C
authors.initials: C
authors.lastname: Collado
authors.forename: V
authors.initials: V
authors.lastname: Oliver
authors.forename: A
authors.initials: A
authors.lastname: Pérez-Pérez
authors.forename: A
authors.initials: A
authors.lastname: Aliaga
mesh_terms: D004279:DNA, Viral / Q000032:analysis; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D016199:Herpesvirus 7, Human / Q000302:isolation & purification*; D019288:Herpesvirus 8, Human / Q000302:isolation & purification; D006801:Humans; D016393:Lymphoma, B-Cell / Q000821:virology*; D016410:Lymphoma, T-Cell, Cutaneous / Q000821:virology*; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies; D012878:Skin Neoplasms / Q000821:virology*
chemical_list: D004279:DNA, Viral