title: Correlation between endotoxin level and bacterial count in bronchoalveolar lavage fluid of ventilated patients.
abstract: OBJECTIVE
  To assess the predictive value of the endotoxin level in the bronchoalveolar lavage (BAL) and to propose to the clinician a guide in the diagnosis of gram-negative bacterial (GNB) pneumonia.
  
  
  METHODS
  Retrospective and prospective studies to investigate the relation between endotoxin level and quantitative bacterial culture of BAL and to test the predictive value of a defined threshold.
  
  
  METHODS
  University hospital general intensive care unit.
  
  
  METHODS
  In the first part of the study, 77 consecutive ventilated patients with clinical suspicion of nosocomial pneumonia between January 1995 and January 1996. In the second part of the study, 93 consecutive ventilated patients studied prospectively between February 1996 and April 1997.
  
  
  RESULTS
  Quantitative cultures for aerobic bacteria were performed directly from the fluid. Bacterial species were determined with standard techniques. The detection of endotoxin in BAL was made using a quantitative chromogenic Limulus assay. In the retrospective analysis, a significant correlation between quantitative GNB cultures and BAL endotoxin levels was observed (r2 = 0.60, p < .0001). An endotoxin level > or = 4 endotoxin units/mL (EU/mL) distinguishes patients with a significant GNB count from colonized patients with a sensitivity of 92.6%, a specificity of 81.4% and a correct classification rate of 84.9%. In the prospective analysis, the 4 EU/mL threshold permits identification of infected patients with a sensitivity of 82.2%, a specificity of 95.6%, and a correct classification rate of 90.3%. The receiver operating characteristic curve analysis showed that the Limulus assay still had a good discrimination power in the prediction of significant bacterial count in BAL fluid.
  
  
  CONCLUSIONS
  Endotoxin detection immediately after bronchoscopy is a distinct advantage to the clinician because antimicrobial gram-negative therapy may be immediately justified according to the results.
authors.affiliation: Department of Anaesthesiology and Intensive Care Medicine, University Hospital of Liège, Belgium. Monique.Nys@chu.ulg.ac.be
authors.forename: M
authors.initials: M
authors.lastname: Nys
authors.forename: D
authors.initials: D
authors.lastname: Ledoux
authors.forename: J L
authors.initials: JL
authors.lastname: Canivet
authors.forename: P
authors.initials: P
authors.lastname: De Mol
authors.forename: M
authors.initials: M
authors.lastname: Lamy
authors.forename: P
authors.initials: P
authors.lastname: Damas
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry* / Q000382:microbiology*; D002648:Child; D015169:Colony Count, Microbial; D004731:Endotoxins / Q000032:analysis*; D005260:Female; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D018410:Pneumonia, Bacterial / Q000175:diagnosis* / Q000382:microbiology; D011237:Predictive Value of Tests; D011446:Prospective Studies; D012121:Respiration, Artificial; D012189:Retrospective Studies
chemical_list: D004731:Endotoxins