title: Identification of the clam species Ruditapes decussatus (Grooved carpet shell), Venerupis pullastra (Pullet carpet shell), and Ruditapes philippinarum (Japanese carpet shell)by PCR-RFLP.
abstract: PCR-RFLP analysis has been applied to the identification of three clam species: Ruditapes decussatus (grooved carpet shell), Venerupis pullastra (pullet carpet shell), and Ruditapes philippinarum (Japanese carpet shell). PCR amplification was carried out using a set of primers designed from the DNA nucleotide sequences reported for alpha-actins from humans and various animals. Restriction endonuclease analysis based on sequence data of the PCR products of each clam species revealed the presence of species-specific polymorphic sites for MaeIII and RsaI endonucleases. Electrophoretic analysis of the amplicons digested with MaeIII and RsaI produced species-specific profiles that allowed the genetic identification of the three clam species.
authors.affiliation: Departamento de Nutrición y Bromatología III, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Fernández
authors.forename: T
authors.initials: T
authors.lastname: García
authors.forename: L
authors.initials: L
authors.lastname: Asensio
authors.forename: M A
authors.initials: MA
authors.lastname: Rodríguez
authors.forename: I
authors.initials: I
authors.lastname: González
authors.forename: A
authors.initials: A
authors.lastname: Céspedes
authors.forename: P E
authors.initials: PE
authors.lastname: Hernández
authors.forename: R
authors.initials: R
authors.lastname: Martín
mesh_terms: D000818:Animals; D001483:Base Sequence; D049872:Bivalvia / Q000145:classification* / Q000235:genetics; D004247:DNA; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
chemical_list: D004247:DNA