Characterization of a monoclonal antibody panel shows that the myotonic dystrophy protein kinase, DMPK, is expressed almost exclusively in muscle and heart.
Myotonic dystrophy (DM) is a multisystemic disorder caused by an inherited CTG repeat expansion which affects three genes encoding the DM protein kinase (DMPK), a homeobox protein Six5 and a protein containing WD repeats. Using a panel of 16 monoclonal antibodies against several different DMPK epitopes we detected DMPK, as a single protein of approximately 80 kDa, only in skeletal muscle, cardiac muscle and, to a lesser extent, smooth muscle. Many earlier reports of DMPK with different sizes and tissue distributions appear to be due to antibody cross-reactions with more abundant proteins. One such antibody, MANDM1, was used to isolate two related protein kinases, MRCK alpha and beta, from a human brain cDNA library and the shared epitope was located at the catalytic site of DMPK using a phage-displayed random peptide library. The peptide library also identified an epitope shared between DMPK and a 55 kDa muscle-specific protein. The results suggest that effects of the repeat expansion on the DMPK gene may be responsible for muscle and heart features of DM, whereas clinical changes in other tissues may be due to effects on the other two genes.
Lam
L T
LT
MRIC Biochemistry Group, PP18, North East Wales Institute, Mold Road, Wrexham LL11 2AW, UK.
Pham
Y C
YC
Nguyen
T M
TM
Morris
G E
GE
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D001483:Base Sequence; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism; D020134:Catalytic Domain; D018076:DNA, Complementary / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism; D009130:Muscle, Smooth / Q000378:metabolism; D009132:Muscles / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D009223:Myotonic Dystrophy / Q000235:genetics / Q000378:metabolism; D065636:Myotonin-Protein Kinase; D019151:Peptide Library; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D019680:Trinucleotide Repeat Expansion
D000911:Antibodies, Monoclonal; C585517:DMPK protein, human; D018076:DNA, Complementary; D000939:Epitopes; D019151:Peptide Library; D065636:Myotonin-Protein Kinase; D017346:Protein Serine-Threonine Kinases