title: Babesia bovis and B. bigemina DNA detected in cattle and ticks from Zimbabwe by polymerase chain reaction.
abstract: From blood collected from 94 cattle at 12 locations in the eastern and northeastern areas of Zimbabwe, DNA was extracted and analysed by polymerase chain reaction with primers previously reported to be specific for Babesia bigemina and Babesia borvis. Overall, DNA of Babesia bigemina was detected in the blood of 33/94 (35%) cattle and DNA from B. bovis was detected in 27/58 (47%) of cattle. The prevalence of DNA of B. bigemina was significantly higher in young animals (<2 years) (23/46) than in animals over 2 years of age (10/48; chi2= 8.77; P <0.01%). Although tick sampling was not thorough, Boophilus decoloratus could be collected at 7/9 sites sampled and Boophilus microplus at 4/9 sites. Of the 20 B. decoloratus allowed to oviposit before PCR analysis, 1 (5%) contained DNA that could be amplified with primers for B. bigemina while 12 (60%) were positive with primers for B. bovis. Of the B. microplus allowed to oviposit, 11/16 (69%) were positive for B. bovis DNA by PCR and 2/16 (12%) were positive for B. bigemina.
authors.affiliation: Division of Parasitology and Tropical Veterinary Medicine, Faculty of Veterinary Medicine, Utrecht University, The Netherlands.
authors.forename: I
authors.initials: I
authors.lastname: Smeenk
authors.forename: P J
authors.initials: PJ
authors.lastname: Kelly
authors.forename: K
authors.initials: K
authors.lastname: Wray
authors.forename: G
authors.initials: G
authors.lastname: Musuka
authors.forename: A J
authors.initials: AJ
authors.lastname: Trees
authors.forename: F
authors.initials: F
authors.lastname: Jongejan
mesh_terms: D017677:Age Distribution; D000818:Animals; D001096:Arachnid Vectors / Q000469:parasitology*; D001403:Babesia / Q000235:genetics / Q000302:isolation & purification*; D016793:Babesia bovis / Q000235:genetics / Q000302:isolation & purification; D001404:Babesiosis / Q000097:blood / Q000453:epidemiology / Q000469:parasitology*; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000453:epidemiology / Q000469:parasitology*; D016054:DNA, Protozoan / Q000097:blood*; D005260:Female; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D013987:Ticks / Q000469:parasitology*; D015030:Zimbabwe / Q000453:epidemiology
chemical_list: D016054:DNA, Protozoan