title: Simultaneous determination of eleven ingredients in ophthalmic solutions by cyclodextrin-modified micellar electrokinetic chromatography with tetrabutylammonium salt.
abstract: Cyclodextrin-modified micellar electrokinetic chromatography was applied to determine simultaneously 11 active ingredients in ophthalmic solutions. All the ingredients were successfully separated by using the mixed carrier system containing sodium dodecyl sulfate with beta-cyclodextrin and tetrabutylammonium phosphate. The effects of the cyclodextrin type on selectivity were also examined. Excellent separation of the all ingredients was obtained by the use of dimethyl-beta-cyclodextrin. The established method was validated and confirmed to be applicable to the determination of the active ingredients in a commercial ophthalmic solution. These results suggest that capillary electrophoresis can be applied to the quantitative analysis as well as qualitative analysis in pharmaceuticals.
authors.affiliation: Analytical Laboratory, Taisho Pharmaceutical Co., Ltd., Omiya-shi, Saitama, Japan. s17127@ccm.taisho.co.jp
authors.forename: H
authors.initials: H
authors.lastname: Okamoto
authors.forename: A
authors.initials: A
authors.lastname: Uetake
authors.forename: R
authors.initials: R
authors.lastname: Tamaya
authors.forename: T
authors.initials: T
authors.lastname: Nakajima
authors.forename: K
authors.initials: K
authors.lastname: Sagara
authors.forename: Y
authors.initials: Y
authors.lastname: Ito
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003505:Cyclodextrins / Q000737:chemistry*; D009883:Ophthalmic Solutions / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*
chemical_list: D003505:Cyclodextrins; D009883:Ophthalmic Solutions; D000644:Quaternary Ammonium Compounds; C009405:tetrabutylammonium