A two dimensional electrophoresis database of a human Jurkat T-cell line.
About 2000 protein spots of human Jurkat T-cells were detected by high resolution two-dimensional gel electrophoresis (2-DE) and were characterized in terms of their isoelectric point and molecular mass. A 2-DE database was constructed and is available at http://www.mpiib-berlin.mpg.de/2D-PAGE/. At present the database contains 67 identified protein spots. These proteins were identified after tryptic digestion by peptide mass fingerprinting with delayed extraction-matrix assisted laser desorption/ionization-mass spectrometry (DE-MALDI-MS). Proteins with a sequence coverage of at least 30% were introduced in the database. This sequence coverage could not always be obtained by using only the matrix alpha-cyano-4-hydroxycinnamic acid (CHCA) for the mass analysis. Therefore, an additional mass spectrum was recorded by using 2,5-dihydroxybenzoic acid (DHB). Usually, additional mass peaks were detected and together with the mass spectrum of CHCA this resulted in the desired sequence coverage.
Thiede
B
B
Max-Planck-Institut für Infektionsbiologie, Berlin, Germany.
Siejak
F
F
Dimmler
C
C
Jungblut
P R
PR
Rudel
T
T
D004396:Coloring Agents; D003373:Coumaric Acids; D016208:Databases, Factual; D015180:Electrophoresis, Gel, Two-Dimensional; D005841:Gentisates; D006801:Humans; D062385:Hydroxybenzoates; D020407:Internet; D007526:Isoelectric Point; D019169:Jurkat Cells / Q000737:chemistry*; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000032:analysis*; D010449:Peptide Mapping; D020543:Proteome; D012394:Rosaniline Dyes; D016622:Silver Staining; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013194:Staining and Labeling
D004396:Coloring Agents; D003373:Coumaric Acids; D005841:Gentisates; D062385:Hydroxybenzoates; D009363:Neoplasm Proteins; D020543:Proteome; D012394:Rosaniline Dyes; C007175:alpha-cyano-4-hydroxycinnamate; C004692:coomassie Brilliant Blue; C010925:2,5-dihydroxybenzoic acid