In vitro cultivation of schizonts of Sarcocystis speeri Dubey and Lindsay, 1999.
Schizonts of Sarcocystis speeri Dubey and Lindsay, 1999 were cultured in vitro in bovine monocyte and equine kidney cell cultures inoculated with infected tissues of nude and gamma-interferon knockout mice fed sporocysts from opossums, Didelphis albiventris. At least 1 asexual cycle was completed in 3 days. In vitro-grown merozoites were structurally and antigenically distinct from those of Sarcocystis neurona and Sarcocystis falcatula. Culture-derived merozoites of S. speeri were not infective to budgerigars (Melopsittacus undulatus).
Dubey
J P
JP
United States Department of Agriculture, Agricultural Research Service, Livestock and Poultry Sciences Institute, Beltsville, Agricultural Research Center, Maryland 20705-2350, USA.
Speer
C A
CA
Lindsay
D S
DS
D000818:Animals; D001921:Brain / Q000469:parasitology; D002417:Cattle; D002460:Cell Line; D002522:Chlorocebus aethiops; D006651:Histocytochemistry; D006736:Horses; D007150:Immunohistochemistry; D007668:Kidney / Q000166:cytology / Q000469:parasitology; D008099:Liver / Q000469:parasitology; D051379:Mice; D018345:Mice, Knockout; D008819:Mice, Nude; D008858:Microscopy, Phase-Contrast; D009000:Monocytes / Q000166:cytology / Q000469:parasitology; D009893:Opossums / Q000469:parasitology; D010311:Parrots; D012522:Sarcocystis / Q000254:growth & development* / Q000472:pathogenicity; D012523:Sarcocystosis / Q000469:parasitology*