An extrachromosomal tetracycline-regulatable system for mammalian cells.
We have modified the tetracycline-regulatable system so that all components are present on a stable extrachromosomal vector that can replicate in a wide variety of mammalian cells. An EBV/human ori vector is used to carry the system, overcoming the species specificity of conventional Epstein-Barr virus vectors. By placing the transcriptional transactivator gene under autoregulation, better induction characteristics are obtained. This system offers greater speed and sensitivity than previously reported methods. It can be applied within 3-4 weeks and produces an induction range of several hundred-fold with a low background.
Sclimenti
C R
CR
Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305-5120, USA.
Baba
E J
EJ
Calos
M P
MP
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002460:Cell Line; D004318:Doxycycline / Q000494:pharmacology*; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D018741:Replication Origin; D015534:Trans-Activators / Q000235:genetics; D014162:Transfection
D000900:Anti-Bacterial Agents; D015534:Trans-Activators; D008156:Luciferases; D004318:Doxycycline