Towards high performance two-dimensional gel electrophoresis using ultrazoom gels.
Proteomics is the analysis of protein expression in cells or tissues, e.g., to study cellular processes at the molecular level. Ultimately, a proteome analysis should encompass most if not all protein species in a biological sample, including those present in low copy numbers. We are developing two-dimensional gel electrophoresis technology by applying narrow pH range ultrazoom gels to enhance resolution and to improve the detection of low abundance proteins. Ultrazoom gels in the acidic pH range allow the detection of proteins down to 300 copies per cell of a B-lymphoma cell line. Protein separation in the alkaline pH range, however, still requires optimization, especially in conjunction with high sample loads.
Hoving
S
S
Novartis Pharma AG, Functional Genomics Area, Protein Sciences Unit, Basel, Switzerland.
Voshol
H
H
van Oostrum
J
J
D004396:Coloring Agents; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D016393:Lymphoma, B-Cell / Q000737:chemistry / Q000473:pathology; D009363:Neoplasm Proteins / Q000302:isolation & purification; D009994:Osmolar Concentration; D020543:Proteome; D012394:Rosaniline Dyes; D012680:Sensitivity and Specificity; D016622:Silver Staining; D013194:Staining and Labeling / Q000379:methods; D014407:Tumor Cells, Cultured / Q000737:chemistry
D004396:Coloring Agents; D009363:Neoplasm Proteins; D020543:Proteome; D012394:Rosaniline Dyes; C048139:Coomassie blue