Establishment of a cell line with phenotypes of chondrocyte from a human osteogenic sarcoma of the mandible.
We have succeeded in transplanting a human osteogenic sarcoma of the mandible into athymic mice. The transplanted tumor showed marked chondrogenesis and mineralization. Recently, a cell line (USAC) with phenotypes of chondrocyte has been established from the transplanted tumor. USAC cells were stellate or spindle-shaped in sparse culture, but polygonal or spherical at sub-confluency to confluency. In long-term culture, the cells were condensed and calcified nodules were formed. Production of types I, II and X collagen were detected by immunohistochemical staining and Western blot analysis. Type I collagen was strongly expressed in the stellate or spindle-shaped cells. Although type II collagen was usually present in all cells during culture, it was strongly stained in polygonal cells at confluency. Type X collagen was seen in large polygonal cells around calcified nodules. Marked [35S]-sulfate uptake and metachromasia were seen at the confluent stage and in the nodule. The cells around the nodules were positive for alkaline phosphatase, and the center of the nodules was stained with alizarin red. The potentiality of cartilage formation was confirmed by in vivo experiments using a diffusion chamber in athymic mice. These observations indicate that USAC cells maintain characteristics of chondrocyte progenitor cells and thus may serve as a useful model to study the sequential events of chondrogenesis and the process of morbid endochondral calcification. This experiment also demonstrated that transplantation of tumor tissue into athymic mice is a convenient strategy for establishment of a cell line.
Yagami
K
K
Second Department of Oral and Maxillofacial Surgery, School of Dentistry, Showa University, Tokyo, Japan.
Kakuta
S
S
Tachibana
H
H
Kimura
Y
Y
Nagumo
M
M
D000704:Analysis of Variance; D000818:Animals; D002455:Cell Division; D002460:Cell Line; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism; D020219:Chondrogenesis; D002999:Clone Cells; D003094:Collagen / Q000096:biosynthesis; D006801:Humans; D007621:Karyotyping; D008339:Mandibular Neoplasms; D051379:Mice; D008819:Mice, Nude; D012516:Osteosarcoma; D010641:Phenotype; D011509:Proteoglycans / Q000096:biosynthesis
D011509:Proteoglycans; D003094:Collagen