cDNA cloning and expression analysis of new members of the mammalian F-box protein family.
F-box proteins are critical components of the SCF ubiquitin-protein ligase complex and are involved in substrate recognition and recruitment for ubiquitination and consequent degradation by the proteasome. We have isolated cDNAs encoding a further 10 mammalian F-box proteins. Five of them (FBL3 to FBL7) share structural similarities with Skp2 and contain C-terminal leucine-rich repeats. The other 5 proteins have different putative protein-protein interaction motifs. Specifically, FBS and FBWD4 proteins contain Sec7 and WD40-repeat domains, respectively. The C-terminal region of FBA shares similarity with bacterial protein ApaG while FBG2 shows homology with the F-box protein NFB42. The marked differences in F-box gene expression in human tissues suggest their distinct role in ubiquitin-dependent protein degradation.
Ilyin
G P
GP
INSERM U522, Hôpital Pontchaillou, Rennes, France. Guennadi.Iline@rennes.inserm.fr
Rialland
M
M
Pigeon
C
C
Guguen-Guillouzo
C
C
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D017173:Caenorhabditis elegans; D003001:Cloning, Molecular; D016208:Databases, Factual; D006801:Humans; D016350:Leucine Zippers; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics* / Q000502:physiology; D010453:Peptide Synthases / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D011506:Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014452:Ubiquitins / Q000378:metabolism; D015003:Yeasts
D011506:Proteins; D014452:Ubiquitins; D010453:Peptide Synthases