Purification and characterization of lipase from psychrophilic Acinetobacter calcoaceticus LP009.
A lipase-producing bacterium, Acinetobacter calcoacetius LP009, was isolated from raw milk. The optimum conditions for growth and lipase production by A. calcoaceticus LP009 were 15 degrees C with shaking at 200 rpm in LB supplemented with 1.0% (v/v) Tween 80. The crude lipase was purified to homogeneous state by ultrafiltration and gel filtration chromatography on Sephadex G-100. Its molecular weight determined by SDS-PAGE was 23 kDa and it exhibited maximum activity at pH 7.0 and 50 degrees C. It was stable over the pH range of 4.0 to 8.0 and at temperatures lower than 45 degrees C. It was a metalloenzyme that is positionally non-specific and had the ability to improve fat hydrolysis in soybean meal and in premixed animals feed.
Pratuangdejkul
J
J
Center for Biotechnology, Institute for Research and Development in Science and Technology, Mahidol University, Salaya, Nakornpathom, Thailand.
Dharmsthiti
S
S
D016954:Acinetobacter calcoaceticus / Q000737:chemistry / Q000201:enzymology* / Q000254:growth & development; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D002413:Cations, Divalent / Q000737:chemistry; D002850:Chromatography, Gel; D003902:Detergents / Q000737:chemistry; D004492:Edetic Acid / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D008892:Milk / Q000382:microbiology*; D012997:Solvents; D013379:Substrate Specificity; D014462:Ultrafiltration
D001426:Bacterial Proteins; D002413:Cations, Divalent; D003902:Detergents; D012997:Solvents; D004492:Edetic Acid; D008049:Lipase