title: Antibodies to the junctional adhesion molecule cause disruption of endothelial cells and do not prevent leukocyte influx into the meninges after viral or bacterial infection.
abstract: A hallmark of infectious meningitis is the invasion of leukocytes into the subarachnoid space. In experimental meningitis triggered by tumor necrosis factor-alpha and interleukin-1beta, the interaction of leukocytes with endothelial cells and the subsequent migration of the cells through the vessel wall can be inhibited by an antibody to the junctional adhesion molecule (JAM). In contrast to the cytokine-induced meningitis model, anti-JAM antibodies failed to prevent leukocyte influx into the central nervous system after infection of mice with Listeria monocytogenes or lymphocytic choriomeningitis virus. Furthermore, in bacterial meningitis, anti-JAM IgG antibodies, but not Fab fragments, caused disruption of the endothelium. Likewise complement-dependent antibody-mediated cytotoxicity was observed in cultured brain endothelial cells treated with anti-JAM IgG but not with its Fab fragment.
authors.affiliation: Cytos Biotechnology AG, CH-8952 Zurich-Schlieren, Switzerland.
authors.forename: F
authors.initials: F
authors.lastname: Lechner
authors.forename: U
authors.initials: U
authors.lastname: Sahrbacher
authors.forename: T
authors.initials: T
authors.lastname: Suter
authors.forename: K
authors.initials: K
authors.lastname: Frei
authors.forename: M
authors.initials: M
authors.lastname: Brockhaus
authors.forename: U
authors.initials: U
authors.lastname: Koedel
authors.forename: A
authors.initials: A
authors.lastname: Fontana
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules / Q000276:immunology*; D004727:Endothelium / Q000276:immunology*; D062726:Junctional Adhesion Molecules; D008089:Listeria monocytogenes / Q000276:immunology; D008088:Listeriosis / Q000276:immunology; D008578:Meninges / Q000276:immunology*; D008581:Meningitis / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D051381:Rats; D019108:Tight Junctions / Q000276:immunology*
chemical_list: D015815:Cell Adhesion Molecules; D062726:Junctional Adhesion Molecules