title: An anchored YAC-STS framework for the rat genome.
abstract: We report here the first YAC-STS framework for the rat genome. A total of 417 anchor microsatellite markers were used to screen a 10-fold redundant YAC library. One or more unambiguous YACs were identified for 372 markers. Assuming the genetic length of the rat genome to be 2,000 cM (Bihoreau et al. 1997b), the YAC-STS framework will provide, on average, one informative YAC clone every 5.4 cM. A total of 111 anchor markers used in this study were derived from known gene regions. We also demonstrated one of the important and immediate uses of this YAC-STS framework, which is to establish a correlation between the genetic and cytogenetic maps in the rat through FISH analysis.
authors.affiliation: Cardiovascular Division, Brigham and Women's Hospital, Department of Cardiology, Children's Hospital, and Department of Medicine, Harvard Medical School, Boston, MA, USA. kl@calvin.bwh.harvard.edu
authors.forename: L
authors.initials: L
authors.lastname: Cai
authors.forename: K
authors.initials: K
authors.lastname: Lindpaintner
authors.forename: J
authors.initials: J
authors.lastname: Browne
authors.forename: F
authors.initials: F
authors.lastname: Gruetzner
authors.forename: T
authors.initials: T
authors.lastname: Haaf
authors.forename: M R
authors.initials: MR
authors.lastname: James
authors.forename: M
authors.initials: M
authors.lastname: Bihoreau
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics*; D018244:Chromosomes, Artificial, Yeast; D016678:Genome; D017404:In Situ Hybridization, Fluorescence; D018895:Microsatellite Repeats; D051381:Rats; D016324:Sequence Tagged Sites