Regulation of cell cycle and apoptosis by human immunodeficiency virus type 1 Vpr.
Biological effects of HIV-1 Vpr on CD4(+) cells were studied by an infection system. High-titered HIV-1 stocks pseudotyped with vesicular stomatitis virus G protein were prepared and used to inoculate into CD4(+ )T cells at high multiplicity of infection. Both cell- and virion-associated Vpr were demonstrated to arrest the cell cycle at the G2/M phase, and to induce cell apoptosis. Of note, morphologically apoptotic cells were shown to be arrested at the G2/M stage. No appreciable effect of Vpr on the anti-Fas antibody-mediated apoptosis was observed in this system.
Fukumori
T
T
Department of Virology, The University of Tokushima School of Medicine, 770-8503, Tokushima, Japan.
Akari
H
H
Yoshida
A
A
Fujita
M
M
Koyama
A H
AH
Kagawa
S
S
Adachi
A
A
D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects*; D002460:Cell Line; D005434:Flow Cytometry; D016195:G2 Phase; D019204:GTP-Binding Proteins / Q000235:genetics; D016336:Gene Products, vpr / Q000494:pharmacology*; D015497:HIV-1; D006367:HeLa Cells; D006801:Humans; D019169:Jurkat Cells; D008938:Mitosis; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics; D054325:vpr Gene Products, Human Immunodeficiency Virus
D016336:Gene Products, vpr; D054325:vpr Gene Products, Human Immunodeficiency Virus; D019204:GTP-Binding Proteins