Organometallic photonucleases: synthesis and DNA-cleavage studies of cyclopentadienyl metal-substituted dendrimers designed to increase double-strand scission.
[reaction: see text] A series of metal complex-substituted polyamine dendrimers have been synthesized and examined for their ability to cleave plasmid DNA in a double-stranded manner. While photolysis of the dimetallic spermine derivative and the tetrametallic DAB-Am-4 complex led to double-strand scission, in the larger DAB-Am-8 and DAB-Am-16 systems, DNA aggregation/precipitation was the predominant competing process observed.
Hurley
A L
AL
Department of Chemistry, Emory University, 1515 Pierce Drive, Atlanta, Georgia 30322, USA.
Mohler
D L
DL
D011232:Chemical Precipitation; D003517:Cyclopentanes / Q000138:chemical synthesis / Q000494:pharmacology; D004247:DNA / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D004249:DNA Damage; D004277:DNA, Single-Stranded / Q000187:drug effects / Q000378:metabolism; D003851:Deoxyribonucleases; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000494:pharmacology*; D010782:Photolysis; D010957:Plasmids / Q000235:genetics; D011073:Polyamines / Q000138:chemical synthesis* / Q000494:pharmacology*; D013096:Spermine / Q000031:analogs & derivatives / Q000138:chemical synthesis
D003517:Cyclopentanes; D004277:DNA, Single-Stranded; D009942:Organometallic Compounds; D011073:Polyamines; D013096:Spermine; D004247:DNA; D003851:Deoxyribonucleases