title: Lactococcus lactis as a cell factory for high-level diacetyl production.
abstract: We report the engineering of Lactococcus lactis for the efficient conversion of sugar into diacetyl by combining NADH-oxidase overproduction and alpha-acetolactate decarboxylase inactivation. Eighty percent of the carbon flux was found to be rerouted via alpha-acetolactate to the production of diacetyl by preloading the cells with NADH-oxidase before their use as a cell factory.
authors.affiliation: Wageningen Centre for Food Sciences and NIZO Food Research, 6710 BA Ede, The Netherlands. hugenhol@nizo.nl
authors.forename: J
authors.initials: J
authors.lastname: Hugenholtz
authors.forename: M
authors.initials: M
authors.lastname: Kleerebezem
authors.forename: M
authors.initials: M
authors.lastname: Starrenburg
authors.forename: J
authors.initials: J
authors.lastname: Delcour
authors.forename: W
authors.initials: W
authors.lastname: de Vos
authors.forename: P
authors.initials: P
authors.lastname: Hols
mesh_terms: D002262:Carboxy-Lyases / Q000037:antagonists & inhibitors; D003931:Diacetyl / Q000378:metabolism*; D005818:Genetic Engineering; D007773:Lactates / Q000378:metabolism; D013294:Lactococcus lactis / Q000235:genetics* / Q000378:metabolism; D009097:Multienzyme Complexes / Q000235:genetics / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics / Q000378:metabolism
chemical_list: D007773:Lactates; D009097:Multienzyme Complexes; C006359:alpha-acetolactate; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases; D002262:Carboxy-Lyases; C023746:acetolactate decarboxylase; D003931:Diacetyl