Successful storage of RBCs for 9 weeks in a new additive solution.
BACKGROUND
This study explored the effect of storing packed RBCs suspended in 200 mL of an alkaline, hypotonic, experimental additive solution (EAS 61).


METHODS
Packed RBC units prepared from RBCs collected from healthy donors in CPD were stored for 8 (n = 10) and 9 (n = 10) weeks under blood bank conditions after the addition of 200 mL of EAS 61 (adenine, 2 mM:; dextrose, 110 mM:; mannitol, 55 mM:; NaCl, 26 mM:; Na(2)HPO(4), 12 mM:). Standard methods were used for in vitro assays. The 24-hour in vivo autologous recoveries were measured with (51)Cr.


RESULTS
Mean +/- SD recoveries at 8 and 9 weeks were 81 +/- 7 and 77 +/- 7 percent. After 9 weeks, the ATP of the RBCs was 81 percent of the initial value, hemolysis was 0.35 percent, supernatant potassium was 46 mEq per L, and the morphologic index was 94.1.


CONCLUSIONS
Packed RBCs suspended in 200 mL of EAS 61 can be stored satisfactorily for 9 weeks. Longer RBC storage should reduce outdating, increase availability of transfusions in remote locations, and improve the efficiency of autologous donor programs.
Hess
J R
JR
Blood Research Detachment, Walter Reed Army Institute of Research, Washington, DC 20307-5100, USA. John.Hess@AMEDD.Army.Mil
Rugg
N
N
Knapp
A D
AD
Gormas
J F
JF
Silberstein
E B
EB
Greenwalt
T J
TJ
D000328:Adult; D001782:Blood Donors; D001793:Blood Preservation; D004912:Erythrocytes; D005260:Female; D006801:Humans; D007038:Hypotonic Solutions / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D009996:Osmotic Fragility / Q000187:drug effects / Q000502:physiology; D013997:Time Factors
D007038:Hypotonic Solutions