TEM-72, a new extended-spectrum beta-lactamase detected in Proteus mirabilis and Morganella morganii in Italy.
A new natural TEM-2 derivative, named TEM-72, was identified in a Proteus mirabilis strain and in a Morganella morganii strain isolated in Italy in 1999. Compared to TEM-1, TEM-72 contains the following amino acid substitutions: Q39K, M182T, G238S, and E240K. Kinetic analysis showed that TEM-72 exhibits an extended-spectrum activity, including activity against oxyimino-cephalosporins and aztreonam. Expression of bla(TEM-72) in Escherichia coli was capable of decreasing the host susceptibility to the above drugs.
Perilli
M
M
Dipartimento di Scienze e Tecnologie Biomediche, Cattedra di Enzimologia, Università di L'Aquila, Italy.
Segatore
B
B
de Massis
M R
MR
Riccio
M L
ML
Bianchi
C
C
Zollo
A
A
Rossolini
G M
GM
Amicosante
G
G
D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D006868:Hydrolysis; D007558:Italy; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D020613:Morganella morganii / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D011513:Proteus mirabilis / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D001618:beta-Lactamases / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D047090:beta-Lactams; C413415:beta-lactamase TEM-72; D001618:beta-Lactamases