title: Vascular and stromal features in the skin of the lower limb in patients with chronic critical limb ischaemia (CLI) and oedema.
abstract: OBJECTIVE
  peripheral oedema is often observed in limbs affected by chronic critical limb ischaemia (CLI) and is mainly subcutaneous in distribution. Previous work has shown that capillary filtration coefficient (CFC) in limbs with CLI and oedema was twice as great as that in the contralateral limb. These changes might be due to morphological changes. Transmission electron microscopy (TEM) was used to examine the morphological features of the capillary walls and surrounding stromal tissues in the skin of these limbs.
  
  
  METHODS
  eight patients with unilateral CLI and peripheral pitting oedema (four men, four women, a mean age of 81+/-6.9 years) was studied. Skin biopsies were taken from the pulp of the first toe, interdigital space between the first and second digits and dorsal part of forefoot just prior to amputation.
  
  
  RESULTS
  stromal oedema and dilated capillaries were most prominent in the distal part of the foot. Some of the capillaries were filled with blood cells and some were empty. The endothelium of the dilated vessels was elongated and distended. In some patients a number of capillaries were collapsed with degenerate endothelial cells. <<<<Gaps>>>>, i.e. large openings, were found between the elongated oedematous endothelial cells. The basal lamina was thickened in all patients. Stromal haemorrhage and degeneration were seen in approximately 50% of patients.
  
  
  CONCLUSIONS
  CLI causes ultrastructural changes in the capillary endothelium and surrounding stroma. The presence of large gaps between endothelial cells as well as an increased capillary pressure may enhance transcapillary transudation, and are most likely the causative factors in the formation of the ischaemic oedema. The stromal haemorrhage as well as degeneration probably signifies a terminal stage of CLI.
authors.affiliation: Department of Vascular Surgery, Akar Hospital, University of Oslo, Norway.
authors.forename: M D
authors.initials: MD
authors.lastname: Anvar
authors.forename: H Z
authors.initials: HZ
authors.lastname: Khiabani
authors.forename: J M
authors.initials: JM
authors.lastname: Nesland
authors.forename: E
authors.initials: E
authors.lastname: Stranden
authors.forename: A J
authors.initials: AJ
authors.lastname: Kroese
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001485:Basement Membrane / Q000648:ultrastructure; D001706:Biopsy; D002196:Capillaries / Q000503:physiopathology / Q000648:ultrastructure*; D002199:Capillary Permeability; D002200:Capillary Resistance; D002908:Chronic Disease; D004487:Edema / Q000209:etiology / Q000473:pathology*; D004730:Endothelium, Vascular / Q000648:ultrastructure; D005260:Female; D006801:Humans; D007511:Ischemia / Q000150:complications / Q000000981:diagnostic imaging / Q000473:pathology*; D007866:Leg / Q000098:blood supply*; D008297:Male; D008854:Microscopy, Electron; D016491:Peripheral Vascular Diseases / Q000150:complications / Q000000981:diagnostic imaging / Q000473:pathology*; D012867:Skin / Q000098:blood supply* / Q000648:ultrastructure*; D017154:Stromal Cells / Q000648:ultrastructure; D014463:Ultrasonography