MK800-62F1, a new inhibitor of apoptotic cell death, from Streptomyces diastatochromogenes MK800-62F1. I. Taxonomy, fermentation, isolation, physico-chemical properties and biological activity.
A new compound, MK800-62F1, was isolated from a cultured broth of Streptomyces diastatochromogenes MK800-62F1. It inhibited H2O2-induced apoptosis in human small cell lung carcinoma Ms-1 cells as well as in human T-cell leukemia Jurkat cells. In addition, MK800-62F1 also inhibited camptothecin-induced apoptosis in Jurkat cells, which was mediated by intracellular H2O2 generation. MK800-62F1 did not exhibit antioxidative activity in vitro, suggesting that inhibition of apoptosis by MK800-62F1 was not due to the scavenging of H2O2, rather it was due to the modulation of the downstream event of H2O2 generation.
Yoshimoto
Y
Y
Department of Applied Chemistry, Faculty of Science and Technology, Keio University, Yokohama, Japan.
Sawa
T
T
Kinoshita
N
N
Homma
Y
Y
Hamada
M
M
Takeuchi
T
T
Imoto
M
M
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D002166:Camptothecin / Q000494:pharmacology; D018288:Carcinoma, Small Cell / Q000188:drug therapy / Q000473:pathology; D004353:Drug Evaluation, Preclinical; D005285:Fermentation; D005452:Fluoresceins / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D019169:Jurkat Cells / Q000187:drug effects; D008175:Lung Neoplasms / Q000188:drug therapy / Q000473:pathology; D015394:Molecular Structure; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D013302:Streptomyces / Q000145:classification / Q000378:metabolism*
D000972:Antineoplastic Agents, Phytogenic; D005452:Fluoresceins; C413564:MK 800-62F1; D012503:Saponins; C029569:diacetyldichlorofluorescein; D006861:Hydrogen Peroxide; D002166:Camptothecin