title: Conditions required for a hybrid artificial liver support system using a PUF/hepatocyte-spheroid packed-bed module and it's use in dogs with liver failure.
abstract: We studied the effects of a hybrid artificial liver support system we developed on dogs with hepatic failure. The system consisted of a multi-channel polyurethane foam packed-bed culture module, including primary dog hepatocyte spheroids. Blood ammonia was well metabolized by 20 g hepatocytes, but the other functions such as glucose concentration, total bile acid concentration, and survival time required 30 g hepatocytes to improve conditions. We found that we should use a culture substratum that easily forms spheroids, and that an artificial liver module should be used as soon as possible after spheroid formation by hepatocytes in the module.
authors.affiliation: Department of Chemical Systems and Engineering, Graduate School of Engineering, Kyushu University, Fukuoka, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ijima
authors.forename: K
authors.initials: K
authors.lastname: Nakazawa
authors.forename: S
authors.initials: S
authors.lastname: Koyama
authors.forename: M
authors.initials: M
authors.lastname: Kaneko
authors.forename: T
authors.initials: T
authors.lastname: Matsushita
authors.forename: T
authors.initials: T
authors.lastname: Gion
authors.forename: K
authors.initials: K
authors.lastname: Shirabe
authors.forename: M
authors.initials: M
authors.lastname: Shimada
authors.forename: K
authors.initials: K
authors.lastname: Takenaka
authors.forename: K
authors.initials: K
authors.lastname: Sugimachi
authors.forename: K
authors.initials: K
authors.lastname: Funatsu
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004285:Dogs; D005260:Female; D022781:Hepatocytes; D017093:Liver Failure / Q000628:therapy*; D019164:Liver, Artificial; D008297:Male; D011140:Polyurethanes
chemical_list: D011140:Polyurethanes; C028279:polyurethane foam