title: Sequence comparison of avian interferon regulatory factors and identification of the avian CEC-32 cell as a quail cell line.
abstract: Interferon (IFN) regulatory factor-1 (IRF-1) is a well-characterized member of the IRF family. Previously, we have cloned cDNA of several members of the chicken IRF (ChIRF) family and studied the function of ChIRF-1 in the avian cell line CEC-32. The IRF-1 proteins from primary chicken embryo fibroblasts (CEF) and CEC-32 cells differed in their electrophoretic mobility. To characterize the different forms of IRF-1 in avian cells, we compared the sequences of IRF-1 cDNA from CEC-32 cells, primary CEF, and quail fibroblasts (QEF). The deduced amino acid sequences of IRF-1 cDNA from chicken and quail show high similarity. Comparison of genomic sequences of IRF-1 and IFN consensus sequence binding protein (ICSBP) also confirm the relatedness of the members of the IRF family in quail and chicken. Based on these data, it is concluded that the avian fibroblast cell line CEC-32 is derived from quail. This conclusion is further supported by deoxynucleotide sequence comparison of a DNA fragment in an avian MHC class II gene and by fluorescence in situ hybridization (FISH) using the vertebrate telomeric (TTAGGG) repeat. Chromosome morphology and the lack of interstitial hybridization signals in macrochromosomes suggest that the CEC-32 cell line has probably been derived from Japanese quail.
authors.affiliation: Institute for Virology and Immunobiology, Würzburg, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Zöller
authors.forename: I
authors.initials: I
authors.lastname: Redman-Müller
authors.forename: I
authors.initials: I
authors.lastname: Nanda
authors.forename: M
authors.initials: M
authors.lastname: Guttenbach
authors.forename: E
authors.initials: E
authors.lastname: Dosch
authors.forename: M
authors.initials: M
authors.lastname: Schmid
authors.forename: R
authors.initials: R
authors.lastname: Zoorob
authors.forename: C
authors.initials: C
authors.lastname: Jungwirth
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002642:Chick Embryo; D003001:Cloning, Molecular; D003370:Coturnix; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D005802:Genes, MHC Class II; D017404:In Situ Hybridization, Fluorescence; D050856:Interferon Regulatory Factor-1; D050835:Interferon Regulatory Factors; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000235:genetics*; D011784:Quail; D012339:RNA, Ribosomal, 28S / Q000235:genetics / Q000302:isolation & purification; D012097:Repressor Proteins / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D000099185:Interferon Regulatory Factor-8
chemical_list: D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D050856:Interferon Regulatory Factor-1; D050835:Interferon Regulatory Factors; D010750:Phosphoproteins; D012339:RNA, Ribosomal, 28S; D012097:Repressor Proteins; D000099185:Interferon Regulatory Factor-8