Quantitative determination of isoflavones and coumestrol in soybean by column liquid chromatography.
Four different stationary phases and a variety of solvents in varying proportions were examined in this study. Daidzein, genistein, formononetin, biochanin A and coumestrol were separated within 24 min on a phenyl column with acetonitrile-water (33:67, v/v) as eluent. The proposed method showed an acceptable repeatability with a RSD of quantitation <6%. The mean recoveries of daidzein, genistein, formononetin, biochanin A and coumestrol from soybean ranged from 89 to 104%. The identity of the individual analytes was confirmed by LC-MS-MS. The four isoflavones and coumestrol were isolated from soybean by hydrolysis with acid and heat. Neutralization of the soybean samples prior to identification did not alter the concentration of daidzein and genistein in soybean.
Hutabarat
L S
LS
Department of Food Science and Technology, University of New South Wales, Sydney, Australia.
Greenfield
H
H
Mulholland
M
M
D002853:Chromatography, Liquid / Q000379:methods*; D003375:Coumestrol / Q000032:analysis*; D007529:Isoflavones / Q000032:analysis*; D013058:Mass Spectrometry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013025:Glycine max / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D007529:Isoflavones; D003375:Coumestrol