Age-related differences in the expression of receptors for TGF-beta in human osteoblast-like cells in vitro.
The cell surface expression of receptors for TGF-beta was studied in human osteoblasts derived from femoral trabecular bone of a total of 19 patients aged 2-83 years. All cell populations investigated showed a similar profile of expression of TGF-beta receptors (TbetaR) I, II and III (betaglycan). There were no significant differences in cell differentiation or proliferative behaviour between the age groups. The TGF-beta receptor number per cell significantly increased with age, while the receptor affinity tended to decrease. IGF-I did not influence TbetaR expression in vitro. The results indicate an age-dependent and IGF-I independent increase of osteoblastic TGF-beta receptors in human osteoblast-like cells in vitro.
Bätge
B
B
Medizinische Klinik I, Medizinische Universität zu Lübeck, Germany. BBaetge@schoen-kliniken.de
Feydt
A
A
Gebken
J
J
Klein
H
H
Notbohm
H
H
Müller
P K
PK
Brinckmann
J
J
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism*; D001667:Binding, Competitive; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010006:Osteoblasts / Q000378:metabolism*; D020033:Protein Isoforms / Q000378:metabolism; D018125:Receptors, Transforming Growth Factor beta / Q000378:metabolism*
D020033:Protein Isoforms; D018125:Receptors, Transforming Growth Factor beta