Analysis of neurotoxin 3-N-oxalyl-L-2,3-diaminopropionic acid and its alpha-isomer in Lathyrus sativus by high-performance liquid chromatography with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) derivatization.
A method was developed for the quantitative determination of the neurotoxic nonprotein amino acid, 3-N-oxalyl-L-2,3-diaminopropionic acid (beta-ODAP), and its nontoxic alpha-isomer, 2-N-oxalyl-L-2, 3-diaminopropionic acid (alpha-ODAP), in the plant samples of Lathyrus sativus after derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) by reversed-phase high-performance liquid chromatography (HPLC). Hippuric acid was used as an internal standard. A linear response was recorded in the concentration rang 0.32-32 nmol with r > 0.999. The RP HPLC detection limit for both isomers is 1.8 ng. According to different experimental needs, a ternary gradient system can be used to determine toxin and other nonprotein amino acids. The RP HPLC method and a colorimetric method were compared for measuring ODAP.
Chen
X
X
National Laboratory of Applied Organic Chemistry and State Key Laboratory of Arid Agroecology, Lanzhou University, Gansu, PRC.
Wang
F
F
Chen
Q
Q
Qin
X C
XC
Li
Z
Z
D000599:Amino Acids, Diamino / Q000032:analysis*; D000634:Aminoquinolines / Q000737:chemistry; D002219:Carbamates / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007887:Fabaceae / Q000737:chemistry*; D007536:Isomerism; D009498:Neurotoxins / Q000032:analysis*; D010946:Plants, Medicinal
C078178:6-aminoquinolyl-N-hydroxysuccinimidyl carbamate; D000599:Amino Acids, Diamino; D000634:Aminoquinolines; D002219:Carbamates; D009498:Neurotoxins; C100126:oxalyldiaminopropionic acid