Overexpression of phospholipase Cbeta-1 protects NIH3T3 cells from oxidative stress-induced cell death.
Oxidative stress has been implicated in a wide range of cellular damage which includes DNA oxidation, membrane lipid peroxidation, and apoptosis. In our study, we found that overexpression of PLC-beta1 in NIH3T3 fibroblasts protected them from cell death occuring in response to oxidative stress. Cell death caused by treatment with prooxidant tert-butylhydroperoxide (TBH), H2O2, or CdCl2 was considerably suppressed in PLC-beta1 overexpressed NIH/beta1-14 cells in comparison to control NIH/neo cells. However, overexpression of PLC-beta1 failed to protect the cells from toxicity by diamide or KCN. In addition, while accumulation of c-fos mRNA was observed within 30 min of TBH treatment in vector transfected NIH/neo cells, TBH-induced c-fos mRNA generation was completely suppressed in NIH/beta1-14 cells, while that of c-jun and GAPDH was not affected. These findings suggest that PLC-beta1 may play a role in process that can protect cells from oxidative stress-induced cell death.
Lee
Y H
YH
Department of Biochemistry, College of Medicine, Yeungnam University, Taegu, South Korea.
Kim
S Y
SY
Kim
J R
JR
Yoh
K T
KT
Baek
S H
SH
Kim
M J
MJ
Ryu
S H
SH
Suh
P G
PG
Kim
J H
JH
D016475:3T3 Cells / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D000818:Animals; D002118:Calcium / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D016762:Genes, fos / Q000187:drug effects / Q000502:physiology; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D051379:Mice; D018384:Oxidative Stress / Q000502:physiology*; D054799:Phospholipase C beta; D014162:Transfection; D010738:Type C Phospholipases / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D020122:tert-Butylhydroperoxide / Q000633:toxicity
D018076:DNA, Complementary; D007527:Isoenzymes; D020122:tert-Butylhydroperoxide; D010738:Type C Phospholipases; D054799:Phospholipase C beta; C518034:Plcb1 protein, mouse; D002118:Calcium