title: Overexpression of GluR6 in rat hippocampus produces seizures and spontaneous nonsynaptic bursting in vitro.
abstract: We hypothesized that overexpression of specific glutamate receptors within the hippocampus would induce seizures and the associated cellular changes seen in temporal lobe epilepsy (TLE). The GluR6 kainate receptor was overexpressed by injecting rat hippocampi with HSVGluR6, a viral vector transducing fully edited GluR6. These animals experienced limbic seizures approximately 4 h following the injection. Control animals injected with HSVlac, a vector expressing beta-galactosidase, did not have seizures. Recordings from hippocampal CA1 pyramidal cells were performed 12 to 48 h and 1 week to 1 month postinjection. We observed nonsynaptic Na(+)-mediated bursting in 77.5% of cells 12 to 48 h following injection of HSVGluR6 but not HSVlac. The synaptic responses were normal in both groups. However, the physiological properties of cells from HSVGluR6-injected hippocampi changed over time. Two weeks following HSVGluR6 injection, synaptic bursts could be evoked, but intrinsic bursting became rare. These changes persisted for at least 1 month. We postulate that this transition from intrinsic to synaptic hyperexcitability may be important in the development of TLE.
authors.affiliation: Department of Neurosurgery, Yale University School of Medicine, New Haven, Connecticut, 06520, USA.
authors.forename: A E
authors.initials: AE
authors.lastname: Telfeian
authors.forename: H J
authors.initials: HJ
authors.lastname: Federoff
authors.forename: P
authors.initials: P
authors.lastname: Leone
authors.forename: M J
authors.initials: MJ
authors.lastname: During
authors.forename: A
authors.initials: A
authors.lastname: Williamson
mesh_terms: D000200:Action Potentials / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D004833:Epilepsy, Temporal Lobe / Q000209:etiology / Q000378:metabolism; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D005822:Genetic Vectors / Q000494:pharmacology; D018259:Herpesvirus 1, Human; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D018092:Kainic Acid Receptors / Q000187:drug effects / Q000378:metabolism*; D012640:Seizures / Q000209:etiology* / Q000378:metabolism; D015222:Sodium Channels / Q000187:drug effects / Q000378:metabolism; D014162:Transfection; D000097806:GluK2 Kainate Receptor
chemical_list: D018691:Excitatory Amino Acid Antagonists; D018092:Kainic Acid Receptors; D015222:Sodium Channels; D000097806:GluK2 Kainate Receptor