Variation in the frequency of micronucleated hepatocytes following time course treatment in the mouse: liver micronucleus assay of partially hepatectomized tissues.
The time-course pattern of the frequency of micronucleated hepatocytes in vivo after partial hepatectomy (PH) was studied in mice using N-nitrosodimethylamine (DMN), N-nitrosodiethylamine (DEN), and 1,2-dimethylhydrazine (DMH), which are rodent liver carcinogens with potent clastogenic activity in the liver. With all chemicals, production of micronucleated hepatocytes was not clearly observed at 3 d after PH, but was clear 4 or 5 d after PH. We propose that it is preferable to perform a preliminary assay prior to the main assay when estimating the clastogenic potential of certain chemicals towards hepatocytes in vivo.
Ohsawa
K
K
Research Center, Taisho Pharmaceutical Co., Ltd., Ohmniya, Saitama, Japan. s12724@ccm.taisho.co.jp
Suzuki
H
H
Hirano
N
N
Hirashio
T
T
Kimura
M
M
D000818:Animals; D006498:Hepatectomy; D022781:Hepatocytes / Q000648:ultrastructure*; D008297:Male; D051379:Mice; D048629:Micronuclei, Chromosome-Defective; D009152:Mutagenicity Tests