Nucleotide-induced movements in the myosin head near the converter region.
Structural changes in subfragment 1 of skeletal muscle myosin were investigated by cross-linking trypsin-cleaved S1 with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide. In the absence of nucleotide the alkali light chains are cross-linked to the 27 kDa heavy chain fragment; the presence of MgATP reduces the efficiency of this reaction. On the other hand, MgATP promotes the cross-link formation between the N-terminal 27 kDa and C-terminal 20 kDa fragments of the heavy chain. The chemical cleavage of the cross-linked heavy chains fragments with N-chlorosuccinimide and hydroxylamine indicates that the cross-links are formed between the regions spanning residues 131-204 and 699-809. These results indicate that the two regions of the heavy chain that are relatively distant in nucleotide-free skeletal S1 [Rayment et al. (1993) Science 261, 50-58] can potentially interact upon addition of nucleotide.
Pliszka
B
B
Department of Muscle Biochemistry, Nencki Institute of Experimental Biology, 3 Pasteur St., PL-02-093 Warsaw, Poland. basiap@nencki.gov.pl
Karczewska
E
E
Wawro
B
B
D000255:Adenosine Triphosphate; D000873:Anthracenes; D003432:Cross-Linking Reagents; D005022:Ethyldimethylaminopropyl Carbodiimide; D005456:Fluorescent Dyes; D008958:Models, Molecular; D018995:Myosin Heavy Chains / Q000737:chemistry; D018994:Myosin Light Chains / Q000737:chemistry; D015879:Myosin Subfragments / Q000737:chemistry; D009218:Myosins / Q000737:chemistry* / Q000235:genetics; D009711:Nucleotides; D010446:Peptide Fragments / Q000737:chemistry; D011487:Protein Conformation; D013388:Succinimides; D014357:Trypsin
D000873:Anthracenes; D003432:Cross-Linking Reagents; D005456:Fluorescent Dyes; D018994:Myosin Light Chains; D015879:Myosin Subfragments; D009711:Nucleotides; D010446:Peptide Fragments; D013388:Succinimides; C015752:N-chlorosuccinimide; C066291:9-anthroylnitrile; D000255:Adenosine Triphosphate; D014357:Trypsin; D018995:Myosin Heavy Chains; D009218:Myosins; D005022:Ethyldimethylaminopropyl Carbodiimide