[5'CpG island methylation of the suppressor gene p16/CDKN2/MTS1 in renal carcinoma].
OBJECTIVE
To determine the methylation status of the tumor suppressor gene p16 in patients with renal carcinoma by analysis of microsatellite polymorphisms.


METHODS
40 patients with sporadic renal cancer were studied. Tumor and normal tissue from each patient were tested for methylation status of the p16 gene by analysis of microsatellite polymorphisms.


RESULTS
20% showed methylation of p16. No correlation was found between this genetic alteration and tumor features.


CONCLUSIONS
Methylation of p16 was found in 20% of the patients in this series. According to the analysis, 18.5% of renal cell carcinomas showed methylation, 50% of chromophobe cell carcinomas and 25% of carcinomas of Bellini's tubules. The rest of the histological types showed no methylation.
Maestro
M L
ML
Servicio de Análisis Clínicos, Hospital Clínico San Carlos, Madrid.
Del Barco
V
V
Sanz-Casla
M T
MT
Moreno
J
J
Zanna
I
I
Redondo
E
E
Fernández
C
C
Resel
L
L
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019175:DNA Methylation; D005260:Female; D019942:Genes, p16 / Q000235:genetics*; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics*; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D008875:Middle Aged; D011446:Prospective Studies