title: A hypotonic storage solution did not prolong the viability of RBCs.
abstract: BACKGROUND
  Hypotonic storage solutions and WBC filtration are both reported to improve RBC viability. This study tested the ability of an investigational hypotonic storage solution (AS-24, Medsep Corp.) to extend the viability of liquid-stored RBCs to 8 weeks.
  
  
  METHODS
  In a pair of crossover trials, 11 RBC units, WBC-reduced by filtration and stored in AS-24 for 8 weeks, were compared with units from the same donors that were stored for 6 weeks in AS-3, and 13 RBC units, WBC-reduced by filtration and stored in AS-3 for 8 weeks, were compared with units from the same donors that were stored for 6 weeks in AS-3. Viability was measured by the (51)Cr/(99m)Tc double-isotope method.
  
  
  RESULTS
  RBC viability at 8 weeks averaged 64 +/- 3 percent in the AS-24 units and 67 +/- 2 percent in the AS-3 units. It was equal at 77 +/- 3 percent and 77 +/- 2 percent after 6 weeks' storage in AS-3 in both trials.
  
  
  CONCLUSIONS
  Prestorage WBC reduction and storage in AS-24 did not extend RBC viability to 8 weeks. The improved viability previously demonstrated with storage of dilute suspensions of RBCs in hypotonic solutions is probably caused by factors other than the hypotonicity.
authors.affiliation: Blood Research Detachment, Walter Reed Army Institute of Research, Washington, DC 20307-5100, USA.
authors.forename: J G
authors.initials: JG
authors.lastname: Babcock
authors.forename: L E
authors.initials: LE
authors.lastname: Lippert
authors.forename: C P
authors.initials: CP
authors.lastname: Derse-Anthony
authors.forename: M
authors.initials: M
authors.lastname: Mechling
authors.forename: J R
authors.initials: JR
authors.lastname: Hess
mesh_terms: D000225:Adenine / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000097:blood; D002470:Cell Survival / Q000187:drug effects; D002951:Citrates / Q000494:pharmacology; D018592:Cross-Over Studies; D004909:Erythrocyte Indices / Q000187:drug effects; D004912:Erythrocytes / Q000737:chemistry / Q000166:cytology*; D005374:Filtration; D005947:Glucose / Q000494:pharmacology; D006461:Hemolysis; D006801:Humans; D007038:Hypotonic Solutions / Q000494:pharmacology*; D007962:Leukocytes; D010710:Phosphates / Q000494:pharmacology; D011310:Preservatives, Pharmaceutical / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: C058647:AS-3 preservative solution; D002951:Citrates; D007038:Hypotonic Solutions; D010710:Phosphates; D011310:Preservatives, Pharmaceutical; D012965:Sodium Chloride; D000255:Adenosine Triphosphate; D005947:Glucose; D000225:Adenine