title: Enhanced expression of translation factor mRNAs in hepatocellular carcinoma.
abstract: Several studies have demonstrated elevated expression of translation factor mRNAs in malignant tissues. In this study, using primary human hepatocellular carcinoma (HCC) tissues, we examined gene expression of translation factors, including 2 eukaryotic initiation factors (eIFs-4A1, -4E), 4 elongation factors (eEFs-1 alpha, -1 gamma, -1 delta, and -2) and 10 ribosomal proteins (Rps P1, P2, S10, L35, L5, L39, L9, L6, S3a and S17), whose mRNA expression has never been examined in HCC. Our results demonstrated that all the mRNAs examined were up-regulated in HCC tissues. Among 7 HCC tissues of different histological grades, the expression of these mRNAs remained at basal levels in a well to moderately differentiated (W/M-) HCC, was coordinately up-regulated in moderately differentiated (M-) HCCs. In moderately to poorly differentiated (M/P-) HCCs, the expression of eEFs-1 gamma, -1 delta, -2, Rps P0 and L9 mRNAs was further up-regulated along with the histological grading. These results therefore suggest that coordination and specific activation of translation factor genes might be involved in the process of liver carcinogenesis.
authors.affiliation: Department of Microbiology and Molecular Virology, Tokyo Medical and Dental University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Shuda
authors.forename: N
authors.initials: N
authors.lastname: Kondoh
authors.forename: K
authors.initials: K
authors.lastname: Tanaka
authors.forename: A
authors.initials: A
authors.lastname: Ryo
authors.forename: T
authors.initials: T
authors.lastname: Wakatsuki
authors.forename: A
authors.initials: A
authors.lastname: Hada
authors.forename: N
authors.initials: N
authors.lastname: Goseki
authors.forename: T
authors.initials: T
authors.lastname: Igari
authors.forename: K
authors.initials: K
authors.lastname: Hatsuse
authors.forename: T
authors.initials: T
authors.lastname: Aihara
authors.forename: S
authors.initials: S
authors.lastname: Horiuchi
authors.forename: M
authors.initials: M
authors.lastname: Shichita
authors.forename: N
authors.initials: N
authors.lastname: Yamamoto
authors.forename: M
authors.initials: M
authors.lastname: Yamamoto
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D005786:Gene Expression Regulation; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D010445:Peptide Elongation Factors / Q000235:genetics; D010448:Peptide Initiation Factors / Q000235:genetics; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000032:analysis*; D012269:Ribosomal Proteins / Q000235:genetics
chemical_list: D010445:Peptide Elongation Factors; D010448:Peptide Initiation Factors; D012333:RNA, Messenger; D012269:Ribosomal Proteins