title: [Construction of reference panel of immune serum against hepatitis C virus with standard level of IgG antibodies].
abstract: A panel of anti-HCV sera (lot 03HC) was prepared from human sera obtained at blood transfusion centers and infectious hospitals. Donor sera and high-titer sera from patients infected with HCV were used. For positive samples, specific sera reactive with the core and/or NS proteins of HCV 1b and 2 were selected. Positive sera were standardized by the concentrations of IgG with a pool of negative sera containing no HBsAg and antibodies to HIV, HCV, and syphilis. The sera for the panel were selected and titered in screening and specific tests. The anti-HCV panel includes negative and positive sera with low and high titers. The panel sera are stabilized and can be stored for a short time at room temperature. The anti-HCV panel of sera, lot 02HC, was certified at L. A. Tarasevich Institute for Standardization and Control as anti-HCV reference panel intended for sensitivity, specificity, and stability control of diagnostic systems for detection of antibodies to HCV in Russia.
authors.forename: A N
authors.initials: AN
authors.lastname: Kanev
authors.forename: N V
authors.initials: NV
authors.lastname: Shalunova
authors.forename: S V
authors.initials: SV
authors.lastname: Netesov
authors.forename: O N
authors.initials: ON
authors.lastname: Iastrebova
authors.forename: E E
authors.initials: EE
authors.lastname: Musina
authors.forename: N G
authors.initials: NG
authors.lastname: Bocharova
authors.forename: V P
authors.initials: VP
authors.lastname: Mishin
authors.forename: N N
authors.initials: NN
authors.lastname: Kiselev
authors.forename: A V
authors.initials: AV
authors.lastname: Shystov
authors.forename: M A
authors.initials: MA
authors.lastname: Smerdova
authors.forename: T A
authors.initials: TA
authors.lastname: Bektimirov
mesh_terms: D000914:Antibodies, Viral / Q000097:blood*; D000918:Antibody Specificity; D016174:Hepacivirus / Q000276:immunology*; D006801:Humans; D007106:Immune Sera; D007118:Immunoassay; D007074:Immunoglobulin G / Q000097:blood*; D012015:Reference Standards
chemical_list: D000914:Antibodies, Viral; D007106:Immune Sera; D007074:Immunoglobulin G