Strongyloides venezuelensis: heparin-binding adhesion substances in immunologically damaged adult worms.
Immunologically damaged Strongyloides venezuelensis adult worms were examined for their mucosal invasion ability and secretion of heparin-binding adhesion substances. S. venezuelensis was expelled from male Wistar rats 4 to 5 weeks after infection. Four-week-old adult worms were smaller and had fewer eggs than 1-week-old adult worms. One-week-old, 4-week-old, and 5-week-old adult worms equally established in the recipient mouse intestine when surgically implanted. Adult worms of 4 and 5 weeks of age secreted adhesion substances as much as 1-week-old adult worms. There was no difference in the heparin-binding activities and the lectin-binding profile of adhesion substances among adult worms of different ages. The rate of secretion of adhesion substances from the mouth was also identical. Heparin-binding activities were detected in crude adult worm proteins; however, proteins of 5-week-old adult worms had weaker heparin-binding activities than those of 1-week-old adult worms. Western blotting revealed that a number of heparin-binding proteins were lost in 5-week-old adult worms. A heparin-binding protein of 42. 0 kDa, which was consistently expressed in adult worms, was a possible component of heparin-binding adhesion substances which are secreted from the mouth.
Maruyama
H
H
Department of Medical Zoology, Nagoya City University Medical School, Nagoya, 467-8601, Japan.
Hatano
H
H
Kumagai
T
T
El-Malky
M
M
Yoshida
A
A
Ohta
N
N
D000818:Animals; D015153:Blotting, Western; D002448:Cell Adhesion / Q000502:physiology; D004386:Duodenum / Q000276:immunology / Q000469:parasitology*; D015801:Helminth Proteins / Q000378:metabolism*; D006493:Heparin / Q000378:metabolism*; D007411:Intestinal Diseases, Parasitic / Q000276:immunology / Q000469:parasitology*; D007814:Larva / Q000276:immunology / Q000378:metabolism; D037102:Lectins / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D051381:Rats; D017208:Rats, Wistar; D013321:Strongyloides / Q000276:immunology / Q000378:metabolism*; D013322:Strongyloidiasis / Q000276:immunology / Q000469:parasitology*
D015801:Helminth Proteins; D037102:Lectins; D006493:Heparin