title: Crystal structure of human survivin reveals a bow tie-shaped dimer with two unusual alpha-helical extensions.
abstract: Survivin is a mitotic spindle-associated protein involved in linking mitotic spindle function to activation of apoptosis in mammalian cells. The structure of the full-length human survivin has been determined by X-ray crystallography to 2.7 A. Strikingly, the structure forms a very unusual bow tie-shaped dimer. It does not dimerize through a C-terminal coiled-coil, contrary to sequence analysis prediction. The C-terminal helices contain hydrophobic clusters with the potential for protein-protein interactions. The unusual shape and dimensions of survivin suggest it serves an adaptor function through its alpha-helical extensions.
authors.affiliation: Laboratoire de Cristallographie Macromoléculaire, Institut de Biologie Structurale Jean-Pierre Ebel, CEA-CNRS, Grenoble, France.
authors.forename: L
authors.initials: L
authors.lastname: Chantalat
authors.forename: D A
authors.initials: DA
authors.lastname: Skoufias
authors.forename: J P
authors.initials: JP
authors.lastname: Kleman
authors.forename: B
authors.initials: B
authors.lastname: Jung
authors.forename: O
authors.initials: O
authors.lastname: Dideberg
authors.forename: R L
authors.initials: RL
authors.lastname: Margolis
mesh_terms: D000595:Amino Acid Sequence; D017209:Apoptosis / Q000502:physiology; D018360:Crystallography, X-Ray; D019281:Dimerization; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D000077022:Survivin
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D011506:Proteins; D011994:Recombinant Proteins; D000077022:Survivin