title: Selective malformation of the splenic white pulp border in L1-deficient mice.
abstract: Lymphocytes enter the splenic white pulp by crossing the poorly characterized boundary of the marginal sinus. In this study, we describe the importance of L1, an adhesion molecule of the Ig superfamily, for marginal sinus integrity. We find that germline insertional mutation of L1 is associated with a selective malformation of the splenic marginal sinus. Other splenic structures remain intact. Immunofluorescence analysis of the extracellular framework of the spleen, using an Ab to laminin, reveals that L1 knockout mice have an irregularly shaped, discontinuous white pulp margin. Electron microscopic analysis shows that it is associated with bizarrely shaped marginal sinus lining cells at the periphery of the white pulp. These abnormalities correlate with the localization of L1 in normal mice in that L1 is normally expressed on marginal sinus lining cells at the white pulp border. These L1-immunopositive lining cells coexpress high levels of mucosal addressin cell adhesion molecule-1 and vimentin, indicating that they are of fibroblastic lineage and express a well-characterized addressin. Our findings are the first to implicate L1 in splenic lymphoid architectural development. Moreover, these findings help define the poorly characterized sinusoidal boundary across which mononuclear cells cross to enter the splenic white pulp.
authors.affiliation: Department of Pathology and Laboratory Medicine, Boston University School of Medicine, Boston, MA 02118, USA.
authors.forename: S L
authors.initials: SL
authors.lastname: Wang
authors.forename: M
authors.initials: M
authors.lastname: Kutsche
authors.forename: G
authors.initials: G
authors.lastname: DiSciullo
authors.forename: M
authors.initials: M
authors.lastname: Schachner
authors.forename: S A
authors.initials: SA
authors.lastname: Bogen
mesh_terms: D000818:Animals; D004912:Erythrocytes / Q000276:immunology; D005109:Extracellular Matrix / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000473:pathology; D005260:Female; D017353:Gene Deletion; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D007150:Immunohistochemistry; D039841:Leukocyte L1 Antigen Complex; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D019006:Neural Cell Adhesion Molecules / Q000096:biosynthesis / Q000235:genetics*; D012756:Sheep; D013154:Spleen / Q000002:abnormalities* / Q000737:chemistry / Q000166:cytology / Q000276:immunology*; D013194:Staining and Labeling
chemical_list: D007074:Immunoglobulin G; D007075:Immunoglobulin M; D039841:Leukocyte L1 Antigen Complex; D008562:Membrane Glycoproteins; D019006:Neural Cell Adhesion Molecules