Establishment and characterization of a cell line (HCDB-1) derived from a hamster buccal pouch carcinoma induced by DMBA and Taiwanese betel quid extract.
This study identified that the carcinogenesis of hamster buccal pouch (HBP) induced by 7,12-dimethylbenz[a]anthracene (DMBA) was greatly enhanced (18 folds) by a combination treatment with Taiwanese betel quid (BQ) extract. A new cell line, HCDB-1, has been established from induced carcinomas. The cultured monolayer cells were epithelioid in shape with irregular nuclei. They demonstrated abundant cytokeratin and tonofilaments; however, ultrastructural well-organized desmosomes were lacking. The HCDB-1 cell exhibited population doubling in 19 h and was highly tumorigenic in nude mice. A C-->T transition at codon 141 (Ala to Val) of the p53 gene was detected in this cell. This mutation is equivalent to a specific temperature-sensitive mouse p53Ala135Val mutant that causes transformation by shifting to 37.5 degrees C. HCDB-1 is the first cell line established from the HBP model of oral carcinogenesis induced by DMBA/Taiwanese BQ extract. It might be valuable for exploring the molecular pathogenesis of oral cancer.
Lin
S C
SC
Institute of Oral Biology and Department of Dentistry, National Yang-Ming University, Taipei, Taiwan, ROC.
Chang
K W
KW
Chang
C S
CS
Yu
S Y
SY
Chao
S Y
SY
Wong
Y K
YK
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D001114:Areca; D002291:Carcinoma, Papillary / Q000139:chemically induced* / Q000235:genetics / Q000473:pathology* / Q000648:ultrastructure; D002455:Cell Division; D006224:Cricetinae; D016158:Genes, p53; D051379:Mice; D009062:Mouth Neoplasms / Q000139:chemically induced* / Q000235:genetics / Q000473:pathology* / Q000648:ultrastructure; D010936:Plant Extracts / Q000633:toxicity*; D010946:Plants, Medicinal; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D013624:Taiwan; D014407:Tumor Cells, Cultured
D010936:Plant Extracts; D015127:9,10-Dimethyl-1,2-benzanthracene