Construction of a multihybrid display system: flagellar filaments carrying two foreign adhesive peptides.
A multivalent, bifunctional flagellum carrying two different adhesive peptides in separate flagellin subunits within a filament was constructed in Escherichia coli. The inserted peptides were the fibronectin-binding 115-mer D repeat region of Staphylococcus aureus and the 302-mer collagen-binding region of YadA of Yersinia enterocolitica. Western blotting, immunoelectron microscopy, and adhesion tests with hybrid flagella from an in trans-complemented DeltafliC E. coli strain showed that individual filaments consisted of both recombinant flagellins.
Tanskanen
J
J
Division of General Microbiology, Department of Biosciences, FIN-00014 University of Helsinki, Finland.
Korhonen
T K
TK
Westerlund-Wikström
B
B
D018829:Adhesins, Bacterial / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001709:Biotechnology / Q000379:methods; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005407:Flagella / Q000378:metabolism*; D005408:Flagellin / Q000235:genetics* / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013211:Staphylococcus aureus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015008:Yersinia enterocolitica / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D002352:Carrier Proteins; D010455:Peptides; D011993:Recombinant Fusion Proteins; C064832:YadA protein, Yersinia; C078635:fibronectin-binding proteins, bacterial; D005408:Flagellin