Isolation and preliminary characterization of the human and mouse homologues of the bacterial cell cycle gene era.
Era is an essential GTPase that is required for proper cell cycle progression and cell division in Escherichia coli and is found in nearly all bacteria sequenced to date. To determine whether Era is also present in eukaryotic organisms, we searched the dbEST database and found EST clones coding for proteins that were similar to Era. Full sequencing of these ESTs from human and mouse identified a conserved homologue, ERAL1 (Era-like 1). ERAL1 maps to 17q11.2 in human and is located in the syntenic region of mouse chromosome 11. ERAL1 may be an attractive candidate for a tumor suppressor gene since ERAL1 is located in a chromosomal region where loss of heterozygosity is often associated with various types of cancer.
Britton
R A
RA
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA.
Chen
S M
SM
Wallis
D
D
Koeuth
T
T
Powell
B S
BS
Shaffer
L G
LG
Largaespada
D
D
Jenkins
N A
NA
Copeland
N G
NG
Court
D L
DL
Lupski
J R
JR
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D002453:Cell Cycle / Q000502:physiology; D002455:Cell Division / Q000235:genetics; D002886:Chromosomes, Human, Pair 17; D003001:Cloning, Molecular; D016208:Databases, Factual; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins; D020224:Expressed Sequence Tags; D020558:GTP Phosphohydrolases / Q000235:genetics* / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D016147:Genes, Tumor Suppressor; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D016601:RNA-Binding Proteins; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D029968:Escherichia coli Proteins; D016601:RNA-Binding Proteins; C068027:era protein, E coli; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins