A new FISH protocol with increased sensitivity for physical mapping with short probes in plants.
Fluorescence in situ hybridization (FISH) is a well-established technique used for the detection of specific DNA regions, that has been applied to interphase nuclei, pachytene and metaphase chromosomes as well as to extended DNA fibres. This technique allows the physical mapping of specific DNA sequences both on individual chromosomes and extended fibres. A new FISH protocol is described here that enhances the sensitivity of the method. Probes for small unique DNA sequences of less than 2 kb give high signal-to-noise ratio with this method, and can be visualized easily by means of conventional fluorescence microscopy.
Guzzo
F
F
University of Verona, Dipartimento Scientifico e Tecnologico, Strada le Grazie 15, Cà Vignal 1, 37134 Verona, Italy.
Campagnari
E
E
Levi
M
M
D027761:Asparagus Plant / Q000235:genetics; D032461:Chromosomes, Plant / Q000235:genetics*; D015342:DNA Probes / Q000235:genetics*; D005434:Flow Cytometry; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D020161:Physical Chromosome Mapping; D012680:Sensitivity and Specificity
D015342:DNA Probes