Reduction in formation of peritoneal adhesions by methylene blue in rats: a dose response study.
OBJECTIVE
To find out if the ability of methylene blue given intraperitoneally to reduce surgically-induced adhesions in rats is concentration dependent.


METHODS
Random, controlled prospective study.


METHODS
Teaching hospital, Israel.


METHODS
Seventy female Wistar rats.


METHODS
Intraperitoneal adhesions were generated in rats by scraping the anterior wall of the uterine horn. They were then given either saline or serially diluted methylene blue intraperitoneally. Additional rats received identical treatments, but without the serosal damage.


METHODS
Graded formation of adhesions, two weeks later.


RESULTS
In the rats treated with methylene blue 1%, adhesion formation was similar to that in sham operated rats and was significantly lower (p < 0.001) than in all other groups.


CONCLUSIONS
Methylene blue 1%, and to lesser degree 0.5%, had the best anti-adhesion potential. Lower methylene blue concentrations were only partially effective, and much high concentrations may cause adverse effects.
Kluger
Y
Y
Department of Surgery B-C, The Rabin Trauma Centre, Sourasky Medical Centre, Tel Aviv, Israel.
Weinbroum
A
A
Ben-Avraham
R
R
Galili
Y
Y
Klausner
J
J
Rabau
M
M
D000005:Abdomen / Q000601:surgery; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D007274:Injections, Intraperitoneal; D008751:Methylene Blue / Q000008:administration & dosage / Q000009:adverse effects / Q000627:therapeutic use*; D010532:Peritoneal Diseases / Q000517:prevention & control*; D011183:Postoperative Complications / Q000517:prevention & control*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D000267:Tissue Adhesions / Q000517:prevention & control
D008751:Methylene Blue