p53 analysis of laryngeal cancer in exon 4 to 9.
p53 gene mutations are a common genetic alteration in human cancer and codon 72/exon 4 polymorphism of the p53 gene has been implicated in cancer risk. Therefore in this study the p53 gene status of 32 shock-frozen tumor specimens from larynx carcinomas was analyzed by PCR and sequencing of exon 4 through 9. Four mutations (12.5%) in exon 5, 7, 8 and 9 were detected in the carcinoma specimen. Analysis of codon 72 revealed in eight cases a homozygosity for proline (CCC) and in 24 cases heterozygosity or homozygosity for arginine (CGC). The group with the proline/proline genotype had a median age 10.3 years lower than the remaining patients and included the only two non-smokers. Firstly, these results confirm the p53 mutational status of laryngeal cancer without any clinical correlation and secondly may suggest an oncogenic potential for the proline/proline genotype of codon 72 for laryngeal cancer as has already been assumed for lung cancer.
Gottschlich
S
S
Department of Otorhinolaryngology, Head and Neck Surgery, University of Kiel, Germany.
Maune
S
S
Preugschat
J
J
Hoffmann
M
M
Werner
J A
JA
Maass
J D
JD
Görögh
T
T
Heidorn
K
K
Rudert
H
H
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001120:Arginine / Q000235:genetics; D003062:Codon; D005091:Exons; D005260:Female; D016158:Genes, p53; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011392:Proline / Q000235:genetics
D003062:Codon; D001120:Arginine; D011392:Proline