Humoral immune response of BALB/c mice to a vaccinia virus recombinant expressing Brucella abortus GroEL does not correlate with protection against a B. abortus challenge.
This work is a part of an ongoing effort to develop vaccinia virus recombinants expressing various Brucella abortus proteins. The B. abortus groEL gene encoding the antigenic heat shock protein GroEL was subcloned into vaccinia virus via homologous recombination and expression confirmed by Western blotting. Female BALB/c mice inoculated with recombinant vaccinia virus/GroEL produced GroEL and vaccinia virus specific antibodies. Mice were challenged 8 weeks post-inoculation with virulent B. abortus strain 2308 and protection measured by the rate of clearance of live Brucella from spleens. Although induction of specific immune response to GroEL and vaccinia virus was demonstrated by the appearance of antibodies in mice, no significant level of protection was demonstrable.
Baloglu
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S
Center for Molecular Medicine and Infectious Diseases, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, 1410 Princes Fork Road, Blacksburg, VA 24061-0342, USA.
Toth
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TE
Schurig
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GG
Sriranganathan
N
N
Boyle
S M
SM
D000818:Animals; D000917:Antibody Formation; D015153:Blotting, Western / Q000662:veterinary; D002003:Brucella abortus / Q000276:immunology*; D002006:Brucellosis / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D018834:Chaperonin 60 / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D014162:Transfection; D014611:Vaccination / Q000662:veterinary; D014616:Vaccinia virus / Q000276:immunology
D018834:Chaperonin 60; D011994:Recombinant Proteins