Molecular studies of the intestinal mucosal barrier physiopathology using cocultures of epithelial and immune cells: a technical update.
Peyer's patch lymphocytes cocultured with Caco-2 cells trigger the phenotypic conversion of enterocytes into cells that express morphological and functional M-cell properties. We report a technical update for setting up this model, which will enable the study of M-cell biology, the identification by biochemical approaches of molecules involved in the interaction of microorganisms with M cells, and the development of vectors that would efficiently target the mucosal immune system.
Kernéis
S
S
Laboratoire des interactions lympho-épitheliales, département de bactériologie et de mycologie, Institut Pasteur, 28, rue du Dr. Roux, 75015, Paris, France.
Caliot
E
E
Stubbe
H
H
Bogdanova
A
A
Kraehenbuhl
J
J
Pringault
E
E
D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D018938:Caco-2 Cells / Q000276:immunology*; D002454:Cell Differentiation; D018920:Coculture Techniques / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000276:immunology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D010581:Peyer's Patches / Q000276:immunology*; D010587:Phagocytosis; D012269:Ribosomal Proteins / Q000032:analysis; D014734:Vibrio cholerae
D001426:Bacterial Proteins; D012269:Ribosomal Proteins