title: Helix deformation is coupled to vectorial proton transport in the photocycle of bacteriorhodopsin.
abstract: A wide variety of mechanisms are used to generate a proton-motive potential across cell membranes, a function lying at the heart of bioenergetics. Bacteriorhodopsin, the simplest known proton pump, provides a paradigm for understanding this process. Here we report, at 2.1 A resolution, the structural changes in bacteriorhodopsin immediately preceding the primary proton transfer event in its photocycle. The early structural rearrangements propagate from the protein's core towards the extracellular surface, disrupting the network of hydrogen-bonded water molecules that stabilizes helix C in the ground state. Concomitantly, a bend of this helix enables the negatively charged primary proton acceptor, Asp 85, to approach closer to the positively charged primary proton donor, the Schiff base. The primary proton transfer event would then neutralize these two groups, cancelling their electrostatic attraction and facilitating a relaxation of helix C to a less strained geometry. Reprotonation of the Schiff base by Asp 85 would thereby be impeded, ensuring vectorial proton transport. Structural rearrangements also occur near the protein's surface, aiding proton release to the extracellular medium.
authors.affiliation: Institut de Biologie Structurale, CEA-CNRS-Université Joseph Fourier, UMR 5075, Grenoble, France.
authors.forename: A
authors.initials: A
authors.lastname: Royant
authors.forename: K
authors.initials: K
authors.lastname: Edman
authors.forename: T
authors.initials: T
authors.lastname: Ursby
authors.forename: E
authors.initials: E
authors.lastname: Pebay-Peyroula
authors.forename: E M
authors.initials: EM
authors.lastname: Landau
authors.forename: R
authors.initials: R
authors.lastname: Neutze
mesh_terms: D001436:Bacteriorhodopsins / Q000737:chemistry* / Q000378:metabolism; D018360:Crystallography, X-Ray; D006860:Hydrogen Bonding; D008958:Models, Molecular; D010777:Photochemistry; D017433:Protein Structure, Secondary; D017494:Proton Pumps / Q000737:chemistry* / Q000378:metabolism
chemical_list: D017494:Proton Pumps; D001436:Bacteriorhodopsins