Simple method of isolation and purification of alpha-galactosides from legumes.
A simple method for the isolation and purification of alpha-galactosides, raffinose family oligosaccharides (RFOs), from legumes has been developed. The method includes (i) imbibition of seeds, (ii) extraction with 50% ethanol, (iii) precipitation of RFOs, (iv) purification of RFOs on diatomaceous earth and charcoal, and (v) cation-exchange chromatography. The described method allows one to obtain high purity RFO preparations (90% for lentil and 80% for pea seeds, determined by HPLC-RI analysis) in the form of white, fine powder. Yields of alpha-galactosides isolated from 100 g of seeds of lentil and pea were 5.6 and 4.3 g, respectively.
Gulewicz
P
P
Department of Biotechnology and Food Microbiology, Agricultural University, Poznań, Poland.
Ciesiołka
D
D
Frias
J
J
Vidal-Valverde
C
C
Frejnagel
S
S
Trojanowska
K
K
Gulewicz
K
K
D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D007887:Fabaceae / Q000737:chemistry*; D005697:Galactosides / Q000302:isolation & purification*; D010946:Plants, Medicinal
D005697:Galactosides