Complete nucleotide sequence and infectious cDNA clone of the RNA1 of a Chinese isolate of broad bean wilt virus 2.
The nucleotide sequence of the RNA1 of broad bean wilt virus 2 (BBWV2) isolate B935 has been determined from overlapping cDNA clones. It contains 5956 nucleotides in length excluding the 3' terminal poly(A) tail and contains a single long open reading frame (ORF) of 5613 nucleotides extending from nucleotide 234 to 5846. A repeated motif has been found in the 5' non-coding region. The predicted polyprotein encoded by the long ORF is 1870 amino acid in length with a molecular weight of 210 K. Amino acid sequence comparisons between portions of the BBWV2 RNA1-encoded polyprotein and proteins encoded by several species in Comoviridae revealed the putative functions of BBWV2 RNA1-encoded proteins and the same general genetic organization as that of comoviruses and nepoviruses. Based on the determined sequence, full-length cDNA clone of RNA1 designated as pU1FL was constructed. Together with transcripts from full-length cDNA clone of RNA2 (pU2FL), transcripts from pU1FL infected Chenopodium quinoa successfully.
Qi
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Institute of Biotechnology, Zhejiang University, Huajiachi, Hangzhou, People's Republic of China. xzhou@mail.hz.zj.cn
Zhou
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Li
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D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary / Q000302:isolation & purification; D007887:Fabaceae / Q000821:virology; D019181:Fabavirus / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010946:Plants, Medicinal; D020815:Polyproteins / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis*; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000032:analysis
D018076:DNA, Complementary; D020815:Polyproteins; D012367:RNA, Viral; D014764:Viral Proteins