title: Neutrophil transmigration across human airway epithelial monolayers: mechanisms and dependence on electrical resistance.
abstract: To examine neutrophil transepithelial migration in the basolateral-to-luminal direction, bronchial epithelial cells (16HBE) were grown at an air-medium interface on the lower face of permeable supports, and resistance across each membrane was recorded before measuring neutrophil transmigration over 2 h. Subconfluent monolayers (resistance < 250 Omega) permitted high spontaneous migration of neutrophils (7.4+/-1%), which was further enhanced (29.7+/-3%) in response to interleukin (IL)-8 (100 ng/ml). Confluent monolayers (250 to 700 Omega) showed low spontaneous migration (2+/- 0.5%) but responded markedly to IL-8 (12.4+/-1.3%). Left in culture, 16HBE resistances continued to increase and were associated with minimal spontaneous migration (< 0.5%) or responses to IL-8. Using cells in the 250 to 700 Omega range, neutrophil migration to IL-8 was dose-dependent and was enhanced when epithelial cells were incubated with a combination of tumor necrosis factor-alpha and interferon-gamma. Neutrophil migration was stimulus-specific and was reduced by preincubation of epithelial cells with a F(ab')(2) anti-intercellular adhesion molecule (ICAM)-1, or by preincubation of neutrophils with anti-CD18, anti-CD11a, anti-CD11b, or anti-CD11c, but not by anti-CD11d, indicating a role for beta(2)-integrin-ICAM-1 interaction in the migration process.
authors.affiliation: Divisions of Pulmonary and Critical Care Medicine and Clinical Immunology, Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
authors.forename: J C
authors.initials: JC
authors.lastname: Kidney
authors.forename: D
authors.initials: D
authors.lastname: Proud
mesh_terms: D000906:Antibodies / Q000494:pharmacology; D001980:Bronchi / Q000166:cytology / Q000276:immunology; D018845:CD11 Antigens / Q000276:immunology; D018929:Cell Culture Techniques / Q000379:methods; D002461:Cell Line, Transformed; D002465:Cell Movement / Q000187:drug effects / Q000276:immunology*; D017097:Electric Impedance; D006801:Humans; D039001:Integrin alpha Chains; D016167:Integrin alphaXbeta2 / Q000276:immunology; D016023:Integrins / Q000276:immunology; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology; D007371:Interferon-gamma / Q000494:pharmacology; D016209:Interleukin-8 / Q000494:pharmacology; D007975:Leukotriene B4 / Q000494:pharmacology; D016169:Lymphocyte Function-Associated Antigen-1 / Q000276:immunology; D016177:Macrophage-1 Antigen / Q000276:immunology; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000166:cytology* / Q000276:immunology*; D020545:Respiratory Mucosa / Q000166:cytology / Q000276:immunology*
chemical_list: D000906:Antibodies; D018845:CD11 Antigens; C406812:ITGAD protein, human; D039001:Integrin alpha Chains; D016167:Integrin alphaXbeta2; D016023:Integrins; D016209:Interleukin-8; D016169:Lymphocyte Function-Associated Antigen-1; D016177:Macrophage-1 Antigen; D018799:Intercellular Adhesion Molecule-1; D007975:Leukotriene B4; D009240:N-Formylmethionine Leucyl-Phenylalanine; D007371:Interferon-gamma