Rapid identification of Enterobacteriaceae using a novel 23S rRNA-targeted oligonucleotide probe.
The aim of this study was to rapidly identify bacteria of the family of Enterobacteriaceae using fluorescent in situ hybridization (FISH). A comparative sequence analysis was carried out and a 23S rRNA signature sequence for Enterobacteriaceae was identified. A 23S rRNA-targeted oligonucleotide probe (EBAC1790) was constructed and subsequently tested against 40 reference strains. Nearly all of the Enterobacteriaceae used in this study yielded positive results with EBAC1790, except for Edwardsiella tarda (ATCC 15947). None of the non-Enterobacteriaceae reference strains gave positive signals with the probe. The possibility of a rapid detection of Enterobacteriaceae in groundwater was demonstrated using colony hybridization.
Bohnert
J
J
Abteilung für Allgemeine Hygiene und Umwelthygiene, Eberhard-Karls-Universität Tübingen, Germany. Juergen.Bohnert@uni-tuebingen.de
Hübner
B
B
Botzenhart
K
K
D004755:Enterobacteriaceae / Q000235:genetics* / Q000302:isolation & purification*; D004784:Environmental Monitoring / Q000379:methods; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015345:Oligonucleotide Probes; D011237:Predictive Value of Tests; D012338:RNA, Ribosomal, 23S / Q000302:isolation & purification*; D017421:Sequence Analysis; D014871:Water Microbiology
D015345:Oligonucleotide Probes; D012338:RNA, Ribosomal, 23S