[The influence of epidermal growth factor, neurotensin on cytosolic calcium and membrane fluidity in carbon tetrachloride-injured primary cultured hepatocytes].
OBJECTIVE
To study the influence of epidermal growth factor (EGF), neurotensin (NT) on cytosolic calcium and membrane fluidity in carbon tetrachloride-injured primary cultured hepatocytes.


METHODS
A micro-model of carbon tetrachloride -induced primary cultured hepatocytes injury was established. EGF and NT were added to the cells 1 h ahead of carbon tetrachloride given. Twenty-four hours later, cytosolic calcium and membrane fluidity in hepatocytes were detected with fluorescence probe of Fura-2/AM and 1, 6-dihenyl-1, 3, 5-hexatriene (DPH).


RESULTS
EGF and NT could significantly antagonize the increase of cytosolic Ca(2+) and decrease of membrane fluidity in carbon tetrachloride-injured hepatocytes.


CONCLUSIONS
EGF and NT have a function of maintaining membrane fluidity and steady-status of cytosolic calcium of hepatocytes.
Zhang
L
L
Institute of Gastrointestinal Disease, Fujian Provincial Hospital, Fuzhou 350001, China.
Cai
L
L
Zheng
Y
Y
Wei
Y
Y
Pan
X
X
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002251:Carbon Tetrachloride / Q000633:toxicity; D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects*; D009496:Neurotensin / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D009496:Neurotensin; D004815:Epidermal Growth Factor; D002251:Carbon Tetrachloride; D002118:Calcium