Expression and genomic characterization of protein phosphatase inhibitor-1: a novel marker for mesothelium in the mouse.
Protein phosphatase inhibitor-1 (inhibitor-1 or I-1) is involved in signal transduction and is an endogenous inhibitor of protein phosphatase-1. The mouse I-1 protein sequence has been deduced from cDNA and is strongly homologous to the published rat sequence. A mouse genomic library was screened, and the I-1 gene was characterized and localized by fluorescent in situ hybridization (FISH) to chromosome 15F. Protein expression in a range of embryonic and adult tissue was analysed using confocal microscopy. Inhibitor-1 is expressed by: the coelomic epithelium; the epithelial bounding layer of cells of the kidney, lung, liver, heart, intestine and gonad; and the surface ectoderm. The blast cells of the kidney do not express I-1. We conclude that I-1 is a marker for mesothelium.
McLaren
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L
Department of Biomedical Sciences and Centre for Developmental Biology, Hugh Robson Building, University of Edinburgh, EH8 9XD, Edinburgh, UK. lornam@holyrood.ed.ac.uk
Boyle
S
S
Mason
J O
JO
Bard
J B
JB
D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins; D004791:Enzyme Inhibitors / Q000378:metabolism; D004848:Epithelium / Q000196:embryology* / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D005819:Genetic Markers; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D047908:Intracellular Signaling Peptides and Proteins; D007668:Kidney / Q000196:embryology / Q000378:metabolism; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors*; D054645:Protein Phosphatase 1; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D002352:Carrier Proteins; D004791:Enzyme Inhibitors; D005819:Genetic Markers; D047908:Intracellular Signaling Peptides and Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C021042:protein phosphatase inhibitor-1; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1