title: The role of higher-order chromatin structure in the yield and distribution of DNA double-strand breaks in cells irradiated with X-rays or alpha-particles.
abstract: OBJECTIVE
  To determine whether the non-random distributions of DNA double-strand breaks in cells observed after alpha-particle irradiation are related to the higher-order structure of the chromatin within the nucleus.
  
  
  METHODS
  Chinese hamster V79 cells were irradiated as either cellular monolayers, nuclear monolayers with condensed chromatin or nuclear monolayers with relaxed chromatin, and the yields and distribution of DSB measured using two pulsed-field gel electrophoresis protocols capable of separating fragments of 10kbp to 5.7 Mbp.
  
  
  RESULTS
  Using conventional FAR analysis, the effect of isolating nuclear monolayers and changing the chromatin condensation state was less for alpha-particle irradiated substrates than for X-irradiated ones. When the total number of breaks was measured by separating and quantifying all the fragments produced in the 10kbp to 5.7Mbp region, the difference between the observed yields of breaks in X-irradiated cells (7.3 x 10(-9) DSB/Gy/bp) relative to alpha-particles (12.1 x 10(-9) DSB/Gy/bp) was largely removed when nuclear monolayers with decondensed chromatin were exposed. The yields, although similar, increased to 44.4 x 10(-9) DSB/Gy/bp for X-irradiated decondensed nuclear monolayers and 46.6 x 10(-9) DSB/Gy/bp for alpha-particle irradiated monolayers. However, the alpha-particle DSB distributions remained non-random.
  
  
  CONCLUSIONS
  Our results suggest that the non-random distribution of breaks observed in cells with alpha-particle irradiation, which leads to a high probability for the production of regionally multiply damaged sites, is not related to the underlying chromatin condensation state present in the nucleus.
authors.affiliation: Gray Laboratory Cancer Research Trust, Mount Vernon Hospital, Northwood, Middlesex, UK.
authors.forename: H C
authors.initials: HC
authors.lastname: Newman
authors.forename: K M
authors.initials: KM
authors.lastname: Prise
authors.forename: B D
authors.initials: BD
authors.lastname: Michael
mesh_terms: D000512:Alpha Particles; D000818:Animals; D002460:Cell Line; D002843:Chromatin / Q000737:chemistry* / Q000528:radiation effects*; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000737:chemistry* / Q000528:radiation effects*; D004249:DNA Damage / Q000528:radiation effects*; D014965:X-Rays
chemical_list: D002843:Chromatin; D004247:DNA