Cathepsin B inhibitory peptides derived from beta-casein.
Two cathepsin B inhibitory peptides were isolated from a commercial pancreatic digest of casein. The peptides were identified as the Pro-Phe-Pro-Gly-Pro-Ile and the Gly-Pro-Phe-Pro-Ile corresponding to the sequence 61-66 and 203-207 of bovine beta-casein. These peptides showed competitive inhibition for cathepsin B with the K(i) values of 2.31 and 3.30 mM, respectively. Two related analogues, Tyr-Pro-Phe-Pro-Gly-Pro-Ile and Val-Tyr-Pro-Phe-Pro-Gly-Pro-Ile, were synthesized but their cathepsin B inhibitory activity was not detected.
Lee
H S
HS
Department of Microbiology, College of Natural Sciences, and Research Centre for Molecular Microbiology, Seoul National University, 151-742, Seoul, South Korea.
Lee
K J
KJ
D002364:Caseins / Q000494:pharmacology*; D002401:Cathepsin B / Q000037:antagonists & inhibitors*; D009842:Oligopeptides / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology
D002364:Caseins; D009842:Oligopeptides; D010446:Peptide Fragments; D002401:Cathepsin B