title: p53 analysis of laryngeal cancer in exon 4 to 9.
abstract: p53 gene mutations are a common genetic alteration in human cancer and codon 72/exon 4 polymorphism of the p53 gene has been implicated in cancer risk. Therefore in this study the p53 gene status of 32 shock-frozen tumor specimens from larynx carcinomas was analyzed by PCR and sequencing of exon 4 through 9. Four mutations (12.5%) in exon 5, 7, 8 and 9 were detected in the carcinoma specimen. Analysis of codon 72 revealed in eight cases a homozygosity for proline (CCC) and in 24 cases heterozygosity or homozygosity for arginine (CGC). The group with the proline/proline genotype had a median age 10.3 years lower than the remaining patients and included the only two non-smokers. Firstly, these results confirm the p53 mutational status of laryngeal cancer without any clinical correlation and secondly may suggest an oncogenic potential for the proline/proline genotype of codon 72 for laryngeal cancer as has already been assumed for lung cancer.
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, University of Kiel, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Gottschlich
authors.forename: S
authors.initials: S
authors.lastname: Maune
authors.forename: J
authors.initials: J
authors.lastname: Preugschat
authors.forename: M
authors.initials: M
authors.lastname: Hoffmann
authors.forename: J A
authors.initials: JA
authors.lastname: Werner
authors.forename: J D
authors.initials: JD
authors.lastname: Maass
authors.forename: T
authors.initials: T
authors.lastname: Görögh
authors.forename: K
authors.initials: K
authors.lastname: Heidorn
authors.forename: H
authors.initials: H
authors.lastname: Rudert
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001120:Arginine / Q000235:genetics; D003062:Codon; D005091:Exons; D005260:Female; D016158:Genes, p53; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011392:Proline / Q000235:genetics
chemical_list: D003062:Codon; D001120:Arginine; D011392:Proline