Intraocular T cells of patients with herpes simplex virus (HSV)-induced acute retinal necrosis recognize HSV tegument proteins VP11/12 and VP13/14.
It has previously been shown that T cells specific for the triggering virus infiltrate the eye of patients with herpes simplex virus type 1 (HSV-1)-induced acute retinal necrosis (ARN). The T cells were mainly directed against 0.67-0.73 HSV-1 map region encoded antigens. The fine specificities of genetically different T cell clones (TCC), obtained from affected eyes of 3 patients with HSV-induced ARN and reactive toward this genomic region of HSV-1, were analyzed with recombinant HSV viruses and synthetic peptides. For 1 patient, the HSV-1 UL46 gene encoded tegument protein VP11/12 was identified as the target antigen. Two separate CD4(+) T cell epitopes were defined in VP11/12. TCC from the other 2 patients recognized the HSV-1 UL47 gene encoded tegument protein VP13/14. Two separate CD4(+) VP13/14 T cell epitopes were identified in these patients. Analysis of the data indicates that HSV-1 VP11/12 and VP13/14 are major target antigens for T cells obtained from vitreous fluid samples of the HSV-induced ARN patients studied.
Verjans
G M
GM
Institute of Virology, Erasmus University Rotterdam, 3000 DR Rotterdam, The Netherlands. Verjans@viro.fgg.eur.nl
Dings
M E
ME
McLauchlan
J
J
van Der Kooi
A
A
Hoogerhout
P
P
Brugghe
H F
HF
Timmermans
H A
HA
Baarsma
G S
GS
Osterhaus
A D
AD
D000956:Antigens, Viral; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D005123:Eye / Q000276:immunology* / Q000473:pathology; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000276:immunology; D006801:Humans; D008213:Lymphocyte Activation; D010641:Phenotype; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology; D015882:Retinal Necrosis Syndrome, Acute / Q000276:immunology* / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology*; D014760:Viral Fusion Proteins / Q000276:immunology*; D014764:Viral Proteins
D000956:Antigens, Viral; D000939:Epitopes; D016693:Receptors, Antigen, T-Cell, alpha-beta; C414831:UL46 protein, Human herpesvirus 1; C085822:VP13-14 protein, herpes simplex virus type 1; D014760:Viral Fusion Proteins; D014764:Viral Proteins