title: Mediator-nucleosome interaction.
abstract: Mediator, a multiprotein complex involved in the regulation of RNA polymerase II transcription, binds to nucleosomes and acetylates histones. Three lines of evidence identify the Nut1 subunit of Mediator as responsible for the histone acetyltransferase (HAT) activity. An "in-gel" HAT assay reveals a single band of the appropriate size. Sequence alignment shows significant similarity of Nut1 to the GCN5-related N-acetyltransferase superfamily. Finally, recombinant Nut1 exhibits HAT activity in an in-gel assay.
authors.affiliation: Department of Structural Biology, Stanford School of Medicine, Stanford, California 94305, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Lorch
authors.forename: J
authors.initials: J
authors.lastname: Beve
authors.forename: C M
authors.initials: CM
authors.lastname: Gustafsson
authors.forename: L C
authors.initials: LC
authors.lastname: Myers
authors.forename: R D
authors.initials: RD
authors.lastname: Kornberg
mesh_terms: D000123:Acetyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051548:Histone Acetyltransferases; D046911:Macromolecular Substances; D056892:Mediator Complex; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D009707:Nucleosomes / Q000378:metabolism*; D012319:RNA Polymerase II / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid
chemical_list: D017931:DNA Primers; D005656:Fungal Proteins; D046911:Macromolecular Substances; D056892:Mediator Complex; D046912:Multiprotein Complexes; C113769:NUT1 protein, S cerevisiae; D009687:Nuclear Proteins; D009707:Nucleosomes; D029701:Saccharomyces cerevisiae Proteins; D000123:Acetyltransferases; D051548:Histone Acetyltransferases; D012319:RNA Polymerase II