Metabolism of some radiolabeled essential fatty acids in isolated rat hepatocytes is affected by dietary ethanol.
The metabolism of the essential fatty acids [1-14C]20:4n-6, [1-14C]20:5n-3 and [1-14C]22:6n-3 was studied in rat hepatocytes fed ethanol in two different diets. Using a diet with a low lipid content ethanol (1) reduced the elongation of eicosapentaenoic acid, (2) reduced the esterification of docosahexaenoic acid (DHA) in phospholipids (PL), (3) increased the oxidation of DHA, (4) increased the ratio of esterification of DHA in phosphatidylethanolamine (PE) compared to phosphatidylcholine (PC) (PE/PC ratio), (5) altered the formation of PL molecular species, and (6) induced a decrease in the endogenous content of the hepatocytes of arachidonic acid and linoleic acid and an increase in oleic acid, 20:3n-9 and DHA. Using a high lipid diet, only the above-mentioned effect (4) was induced by ethanol, not the effects (1)-(3) and (5)-(6).
Retterstøl
K
K
Institute of Clinical Biochemistry, University of Oslo, Rikshospitalet, Norway. kjetil.retterstol@rh.uio.no
Lund
A M
AM
Tverdal
S
S
Cristophersen
B O
BO
D000818:Animals; D002492:Central Nervous System Depressants / Q000494:pharmacology*; D004041:Dietary Fats / Q000378:metabolism / Q000494:pharmacology*; D004281:Docosahexaenoic Acids / Q000378:metabolism*; D000431:Ethanol / Q000494:pharmacology*; D005228:Fatty Acids, Essential / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D002492:Central Nervous System Depressants; D004041:Dietary Fats; D005228:Fatty Acids, Essential; D004281:Docosahexaenoic Acids; D000431:Ethanol