A rapid procedure for the isolation of plasmid DNA from environmental bacteria.
The INSTA-MINI-PREP method, a rapid protocol for plasmid DNA extraction, was originally developed to prepare plasmid DNA from 1 to 3 ml miniprep Escherichia coli cultures. Direct extraction of plasmid DNA is achieved by a two-phase solution which is separated by centrifugation in the presence of the INSTA-PREP gel barrier material. This method has been successfully tested on various environmental Salmonella strains, although it was not suitable for Pseudomonas aeruginosa and enterococci strains. The INSTA-MINI-PREP method is a new alternative procedure to screen plasmid contents of Salmonella and E. coli strains rapidly and easily.
Ferrús
M A
MA
Department of Biotechnology, Polytechnic University of Valencia, Spain. mferrus@btc.upv.es
Alonso
J L
JL
Amorós
I
I
Hernández
M
M
Hernández
J
J
D001431:Bacteriological Techniques; D002498:Centrifugation / Q000379:methods; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics; D005618:Fresh Water; D005782:Gels; D010957:Plasmids / Q000235:genetics*; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000302:isolation & purification; D012475:Salmonella / Q000235:genetics* / Q000302:isolation & purification; D014871:Water Microbiology; D014876:Water Pollution
D004269:DNA, Bacterial; D005782:Gels