title: Regeneration of functional bladder substitutes using large segment acellular matrix allografts in a porcine model.
abstract: OBJECTIVE
  We previously reported on the short-term (4 weeks) morphometric analysis of a large bladder acellular matrix allograft used as a bladder bioprosthesis (average size 24 cm.2). We demonstrated cellular repopulation through the entire thickness of the graft. We now present the long-term (12 weeks) morphometric results of graft regenerated porcine bladders using segments measuring an average of 40 cm.2.
  
  
  METHODS
  Bladders harvested from pigs were subjected to detergent and enzymatic extractions to render them acellular. Partial cystectomy was performed in 21 pigs and the defect was repaired with a bladder acellular matrix allograft (average size 40.52 cm.2). Of the animals 8 were sacrificed at 1, 2 and 4 weeks and 13 were sacrificed at 8 and 12 weeks. To evaluate cellular repopulation and matrix reorganization the native bladder and graft were analyzed using standard histological and immunofluorescent techniques. To evaluate for calcium deposits in the grafts a radiological evaluation of the graft was performed after explantation.
  
  
  RESULTS
  All animals survived the surgical procedure and there were no significant urinary leaks. No stones were noted in any of the bladders. At 1 week there was a diffuse infiltration with acute inflammatory cells. At 2 weeks the luminal surface of the graft was lined with a single layer of urothelium, and there was stromal infiltration with unorganized smooth muscle cells and angiogenesis. At 4 weeks the urothelium was multilayered with organizing groups of smooth muscle cells and angiogenesis. At 8 and 12 weeks there was repopulation throughout the bladder acellular matrix allograft implant with all native cellular components participating.
  
  
  CONCLUSIONS
  We present evidence that large patch bladder acellular matrix allograft implantation is technically feasible and may prove to be a viable surgical alternative to bladder augmentation with intestinal segments. Its advantages may include the potential for complete and functional regeneration of a bladder substitute.
authors.affiliation: Division of Urology, Department of Surgery, The Hospital for Sick Children, University of Toronto, Ontario, Canada.
authors.forename: P P
authors.initials: PP
authors.lastname: Reddy
authors.forename: D J
authors.initials: DJ
authors.lastname: Barrieras
authors.forename: G
authors.initials: G
authors.lastname: Wilson
authors.forename: D J
authors.initials: DJ
authors.lastname: Bägli
authors.forename: G A
authors.initials: GA
authors.lastname: McLorie
authors.forename: A E
authors.initials: AE
authors.lastname: Khoury
authors.forename: P A
authors.initials: PA
authors.lastname: Merguerian
mesh_terms: D000818:Animals; D005240:Feasibility Studies; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D019651:Plastic Surgery Procedures; D013552:Swine; D014184:Transplantation, Homologous; D001743:Urinary Bladder / Q000473:pathology / Q000601:surgery*; D013520:Urologic Surgical Procedures; D019459:Urothelium / Q000473:pathology