Retroviral-mediated gene transfer in primary murine and human T-lymphocytes.
Recombinant retroviruses are efficient vectors for introducing genes into many mammalian cell types. They are useful in the context of clinical as well as experimental applications, owing to the ability to generate high-titer and helper-free viral stocks. Retroviral vectors are especially appropriate for the transduction of primary lymphocytes, because gene transfer is stable and mediated by nonimmunogenic vectors. Stable integration in chromosomes of cells undergoing clonal expansion ensures that the foreign genetic material will be faithfully transmitted to the cells' progeny. However, oncoretroviral vectors derived from murine leukemia viruses (MLV) require target cell division to integrate. Here we review factors that determine retroviral-mediated gene transfer efficiency in primary T-lymphocytes, in particular, T-cell activation status, viral receptor expression, and culture conditions.
Rivière
I
I
Department of Human Genetics, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.
Gallardo
H F
HF
Hagani
A B
AB
Sadelain
M
M
D026923:Amino Acid Transport Systems, Basic; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D002470:Cell Survival; D002478:Cells, Cultured; D005434:Flow Cytometry; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D009052:Leukemia Virus, Murine / Q000235:genetics; D008213:Lymphocyte Activation / Q000502:physiology; D008565:Membrane Proteins / Q000378:metabolism; D028044:Phosphate-Binding Proteins; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism; D012190:Retroviridae / Q000235:genetics*; D013601:T-Lymphocytes / Q000166:cytology / Q000502:physiology* / Q000821:virology*; D014162:Transfection / Q000379:methods*
D026923:Amino Acid Transport Systems, Basic; D002352:Carrier Proteins; D008565:Membrane Proteins; D028044:Phosphate-Binding Proteins; D011991:Receptors, Virus