title: Effect of nitric oxide synthase inhibitor on experimentally induced burn wounds.
abstract: BACKGROUND
  Nitric oxide (NO) may have an important role in the healing of burn wounds. This study investigated the effect of NO on experimentally induced burn wounds by preventing NO synthesis.
  
  
  METHODS
  A total of 40 mice weighing 25 to 30 g were used in this study. The shaved skin on the back of the mice was immersed in 100 degrees C water for 10 seconds to achieve a partial-thickness scald burn. The mice were divided into two groups of 20. In group I (control group), 17.5 mg/kg of serum physiologic (placebo) was injected intraperitoneally two times a day for 15 days. In group II (study group), 17.5 mg/kg of aminoguanidine (NO synthase inhibitor) was injected intraperitoneally two times a day for 15 days. On day 15 of the burn, the animals were killed and the burn areas were investigated histologically. Histologic changes such as epithelial proliferation, abscess, collagen, and granulation tissue were evaluated.
  
  
  RESULTS
  Epithelial proliferation, formation of collagen, and granulation tissue with rich capillaries observed in the control group were statically significantly higher than those observed in the study group (z = -2.022, p < 0.05; z = -2.02, p < 0.05; and z = -2.022, p < 0.05; respectively).
  
  
  CONCLUSIONS
  We concluded that healing of the burn wound is delayed by preventing NO synthesis.
authors.affiliation: Department of General Surgery, Atatürk University Medical School, Erzurum, Turkey.
authors.forename: M N
authors.initials: MN
authors.lastname: Akçay
authors.forename: O
authors.initials: O
authors.lastname: Ozcan
authors.forename: C
authors.initials: C
authors.lastname: Gündoğdu
authors.forename: G
authors.initials: G
authors.lastname: Akçay
authors.forename: A
authors.initials: A
authors.lastname: Balik
authors.forename: K
authors.initials: K
authors.lastname: Köse
authors.forename: D
authors.initials: D
authors.lastname: Oren
mesh_terms: D000818:Animals; D002056:Burns / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D004791:Enzyme Inhibitors / Q000008:administration & dosage / Q000494:pharmacology*; D006146:Guanidines / Q000008:administration & dosage / Q000494:pharmacology*; D007274:Injections, Intraperitoneal; D051379:Mice; D009569:Nitric Oxide / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D012867:Skin / Q000187:drug effects / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
chemical_list: D004791:Enzyme Inhibitors; D006146:Guanidines; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; C004479:pimagedine