[Low molecular weight heparin (LMWH) modulates migration of peripheral blood mononuclear cells and neutrophils of asthmatics].
Evidence has now accumulated that heparin can significantly affect immune response including allergic inflammation. Cell migration is supposed to be very crucial in this process. Thus the aim of that study was to investigate whether low molecular weight heparin-nadroparine is chemoattractant for some inflammatory cells in asthmatics. Peripheral blood mononuclear cells (PBMC) and neutrophils from 15 asthmatics were obtained by gradient centrifugation. Chemotaxis was compared with that induced with known chemoattractants-fMLP and IL-8. We found that nadroparine caused significant and dose dependent chemotaxis of PBMC and comparable with influence of fMLP and IL-8. Nadroparine amplified chemotaxis of neutrophils but not significantly. Chemotaxis induced by fMLP and IL-8 was diminished when blood cells were incubated earlier with 50 mg of nadroparine.
Krasnowska
M
M
Katedra i Klinika Chorób Wewnetrznych i Alergologii AM we Wrocławiu.
Zak-Nejmark
T
T
Krasnowski
R
R
D000328:Adult; D000368:Aged; D001249:Asthma / Q000188:drug therapy / Q000276:immunology; D002633:Chemotaxis / Q000187:drug effects*; D005260:Female; D005343:Fibrinolytic Agents / Q000493:pharmacokinetics* / Q000627:therapeutic use; D006495:Heparin, Low-Molecular-Weight / Q000493:pharmacokinetics* / Q000627:therapeutic use; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D017762:Nadroparin / Q000493:pharmacokinetics* / Q000627:therapeutic use; D009504:Neutrophils / Q000378:metabolism*
D005343:Fibrinolytic Agents; D006495:Heparin, Low-Molecular-Weight; D016209:Interleukin-8; D017762:Nadroparin