Immunization with recombinant surface antigens p26 with Freund's adjuvants against Babesia rodhaini infection.
The surface proteins of Babesia rodhaini have previously been shown to induce a high degree of protective immunity. In the present study, one of those proteins, B. rodhaini antigen p26 was expressed in Escherichia coli and in insect cells infected with a recombinant baculovirus. These proteins were recognized by immune serum from a drug-cured BALB/c mouse. While BALB/c mice immunized with both recombinant antigens and Freund's adjuvants showed 40-100% survival rate against challenge infection with B. rodhaini, saponin failed to induce protection, although significant levels of B. rodhaini-specific antibodies were produced in both immunized mice (1:1,000-2,000 by indirect immunofluorescent antibody test). The immunization of IFN-gamma-deficient mice with the recombinant proteins was not protective against B. rodhaini infection, indicating that IFN-gamma is one of the important factors for the survival against lethal B. rodhaini infection.
Igarashi
I
I
The Research Center for Protozoan Molecular Immunology, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
Asaba
U
U
Xuan
X
X
Omata
Y
Y
Saito
A
A
Nagasawa
H
H
Fujisaki
K
K
Suzuki
N
N
Iwakura
Y
Y
Mikami
T
T
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D001403:Babesia / Q000276:immunology*; D001404:Babesiosis / Q000276:immunology* / Q000517:prevention & control; D003001:Cloning, Molecular; D004912:Erythrocytes / Q000469:parasitology; D004926:Escherichia coli; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005620:Freund's Adjuvant; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D019444:Vaccines, DNA / Q000276:immunology*
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D007074:Immunoglobulin G; D019444:Vaccines, DNA; D005620:Freund's Adjuvant