The p73 gene is not mutated in oligodendrogliomas which frequently have a deleted region at chromosome 1p36.3.
An allelic loss of the chromosome 1p36 region is frequently found in oligodendrogliomas, which suggests the presence of putative tumor suppressor gene(s) in the region. Since the p73 gene, which encodes a protein with significant homology with p53, is mapped to the 1p36.33 region, we examined genetic alterations of the p73 gene in oligodendrogliomas. We screened 10 specimens for mutation throughout the p73 coding regions by polymerase chain reaction (PCR)-single strand conformation polymorphism analysis and by sequencing aberrantly migrated PCR products. We found several polymorphic nucleotide changes, but no somatic mutations that caused an amino acid change. The p73 gene is thus unlikely to be a tumor suppressor gene for oligodendrogliomas.
Tsujimoto
T
T
Department of Biology, Toho University, Chiba, Japan.
Mochizuchi
S
S
Iwadate
Y
Y
Namba
H
H
Nagai
M
M
Kawamoto
T
T
Sunahara
M
M
Yamaura
A
A
Nakagawara
A
A
Sakiyama
S
S
Tagawa
M
M
D002872:Chromosome Deletion; D002878:Chromosomes, Human, Pair 1; D004268:DNA-Binding Proteins / Q000235:genetics*; D016147:Genes, Tumor Suppressor; D006801:Humans; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics*; D009837:Oligodendroglioma / Q000235:genetics*; D016133:Polymerase Chain Reaction; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C000606460:TP73 protein, human; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins