title: Antibodies to parvovirus B19 non-structural protein are associated with chronic but not acute arthritis following B19 infection.
abstract: OBJECTIVE
  To determine the incidence and significance of antibodies to the parvovirus B19 non-structural (NS1) protein in B19-infected persons during acute infection and convalescence.
  
  
  METHODS
  The B19 NS1 protein was expressed in SF9 cells using the baculovirus expression system and was used to prepare immunofluorescence slides. These were used in a fluorescent antibody test to determine anti-B19 NS1 IgG in a well-characterized cohort of 53 persons at the time of acute B19 infection and again after a follow-up period of 26-85 months. Results were examined for statistical significance by the use of Fisher's exact test.
  
  
  RESULTS
  NS1 antibodies were detected in five of 32 persons with acute B19 infection (four with arthritis) and 10 of 53 persons with past B19 infection (six with chronic arthritis and two with chronic arthritis and chronic fatigue syndrome). Regarding the correlation of NS1 antibodies and arthritis, at the time of acute infection four of 24 persons with arthritis had NS1 antibodies detected compared with one of eight persons with any other symptoms (P: = 1). During convalescence, eight of 20 persons with chronic arthritis had NS1 antibodies compared with two of 33 with symptoms of any other category (all except one were asymptomatic) (P: = 0.007). All 10 patients with NS1 antibodies during convalescence had arthritis during acute infection, which persisted in eight persons until the time of follow-up.
  
  
  CONCLUSIONS
  Antibodies to parvovirus B19 NS1 protein are associated with chronic but not with acute arthritis after B19 infection.
authors.affiliation: Departments of Medical Microbiology and Virology, Manchester Royal Infirmary, Oxford Road, Manchester M13 9WL, UK.
authors.forename: J R
authors.initials: JR
authors.lastname: Kerr
authors.forename: V S
authors.initials: VS
authors.lastname: Cunniffe
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000032:analysis*; D001168:Arthritis / Q000276:immunology* / Q000382:microbiology*; D002648:Child; D002908:Chronic Disease; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010322:Parvoviridae Infections; D016732:Parvovirus B19, Human / Q000276:immunology*; D017361:Viral Nonstructural Proteins / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; C106202:NS1 protein, parvovirus; D017361:Viral Nonstructural Proteins