Matrix-assisted laser desorption/ionization time-of-flight analysis of Bacillus spores using a 2.94 microm infrared laser.
The performance of infrared (2.94 microm) and ultraviolet (337 nm) lasers were compared for analysis of purified spores of B. subtilis, B. cereus and B. globigii on a four-inch end-cap reflectron time-of-flight instrument. Infrared matrix-assisted laser desorption/ionization (IR-MALDI) mass spectra of these microorganisms displayed a larger number of biomarker peaks above m/z 4000, compared with UV-MALDI. Biomarker peaks were observed at higher m/z values with the IR laser.
Ryzhov
V
V
Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA.
Bundy
J L
JL
Fenselau
C
C
Taranenko
N
N
Doroshenko
V
V
Prasad
C R
CR
D001407:Bacillus / Q000737:chemistry*; D007259:Infrared Rays; D007834:Lasers; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013171:Spores, Bacterial / Q000737:chemistry