Molecular cloning, expression, and antigenicity of Seto virus belonging to genogroup I Norwalk-like viruses.
The viral capsid protein of the Seto virus (SeV), a Japanese strain of genogroup I Norwalk-like viruses (NLVs), was expressed as virus-like particles using a baculovirus expression system. An antigen detection enzyme-linked immunosorbent assay based on hyperimmune antisera to recombinant SeV was highly specific to homologous SeV-like strains but not heterologous strains in stools, allowing us type-specific detection of NLVs.
Kobayashi
S
S
Laboratory of Virology, Aichi Prefectural Institute of Public Health, Tujimachi, Kita-ku, Nagoya 462-8576, USA. shinkoba@he.mirai.ne.jp
Sakae
K
K
Suzuki
Y
Y
Shinozaki
K
K
Okada
M
M
Ishiko
H
H
Kamata
K
K
Suzuki
K
K
Natori
K
K
Miyamura
T
T
Takeda
N
N
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D015139:Blotting, Southern; D002139:Caliciviridae / Q000145:classification* / Q000235:genetics / Q000276:immunology*; D017250:Caliciviridae Infections / Q000821:virology*; D002213:Capsid / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D009663:Norwalk virus / Q000145:classification / Q000235:genetics / Q000276:immunology; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000956:Antigens, Viral; D011994:Recombinant Proteins