Sequence of 16S rRNA gene of rat-origin cilia-associated respiratory (CAR) bacillus SMR strain.
The 16S rRNA gene of the SMR strain of cilia-associated respiratory (CAR) bacillus, which was isolated from a spontaneously infected rat at our institute, was sequenced. Its 1,521 nucleotides were determined. On the basis of the results of the sequence analysis, the SMR strain was found to be most closely related to members of the Flavobacter/Flexibacter group. This sequence was compared with the previously determined 16S rRNA gene sequences (rat-origin: three; mouse-origin: one; rabbit-origin: one) of CAR bacillus isolates. The SMR strain showed the highest sequence similarity (99.9%) to the rat-origin CARB-NIH strain (Schoeb et al., 1993), and it was concluded that the strains are identical.
Kawano
A
A
Division of Education and Scientific Services, National Institute of Radiological Sciences, Chiba-shi, Japan.
Nenoi
M
M
Matsushita
S
S
Matsumoto
T
T
Mita
K
K
D000818:Animals; D000830:Animals, Laboratory; D001407:Bacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001483:Base Sequence; D002923:Cilia / Q000382:microbiology; D017931:DNA Primers; D004275:DNA, Ribosomal / Q000235:genetics*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology / Q000662:veterinary*; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D051381:Rats; D020545:Respiratory Mucosa / Q000382:microbiology; D012376:Rodent Diseases / Q000382:microbiology*; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
D017931:DNA Primers; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S