Regeneration of PAPS for the enzymatic synthesis of sulfated oligosaccharides.
This paper describes the study of 3'-phosphoadenosine-5'-phosphosulfate (PAPS) regeneration from 3'-phosphoadenosine-5'-phosphate (PAP) for use in practical syntheses of carbohydrate sulfates which are catalyzed by sulfotransferases. Among the regeneration systems, the one with recombinant aryl sulfotransferase proved to be the most practical. This regeneration system was coupled with a sulfotransferase-catalyzed reaction, using a recombinant Nod factor sulfotransferase, for the synthesis of various oligosaccharide sulfates that were further glycosylated using glycosyltransferases.
Burkart
M D
MD
Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
Izumi
M
M
Chapman
E
E
Lin
C H
CH
Wong
C H
CH
D015239:Arylsulfotransferase / Q000737:chemistry*; D001483:Base Sequence; D002240:Carbohydrate Sequence; D002384:Catalysis; D017931:DNA Primers; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000138:chemical synthesis* / Q000737:chemistry; D010724:Phosphoadenosine Phosphosulfate / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry; D013464:Sulfuric Acids / Q000737:chemistry
D017931:DNA Primers; D009844:Oligosaccharides; D011994:Recombinant Proteins; D013464:Sulfuric Acids; D010724:Phosphoadenosine Phosphosulfate; D015239:Arylsulfotransferase