Studying heterotrimeric G-protein-linked signal transduction using replication-deficient adenoviruses.
Plasma membrane-spanning G-protein-linked receptors transduce approximately 60% of all extracellular stimuli in higher animals. Many G-protein-linked receptor pathways are yet to be elucidated, with the receptor, G-protein or effector system as yet unidentified. In addition, many fundamental issues pertaining to G-protein signalling remain unresolved, such as the factors governing the specificity of G-protein receptor coupling and the control of signal amplitude in response to G-protein activation. In order to address some of these issues, the use of replication-deficient adenoviruses as gene transfer vectors for investigations of G-protein signalling has been developed, facilitating dissection of G-protein-linked signal transduction pathways in an extensive range of cultured cells, as well as in vivo. The present review focuses on the versatility and utility of adenoviruses for the investigation of signalling by heterotrimeric G-proteins and explores some of the recent advances in adenoviral technology as they relate to the study of signal transduction.
Cummins
M M
MM
Department of Physiology, University of Sydney, New South Wales, Australia.
Poronnik
P
P
O'Mullane
L M
LM
Cook
D I
DI
D000256:Adenoviridae / Q000235:genetics* / Q000254:growth & development; D000809:Angiotensins / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D004705:Endocytosis; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000502:physiology; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D020962:Heterotrimeric GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D011941:Receptors, Adrenergic / Q000378:metabolism; D011966:Receptors, LHRH / Q000378:metabolism; D011976:Receptors, Muscarinic / Q000378:metabolism; D018035:Receptors, Odorant / Q000378:metabolism; D018048:Receptors, Purinergic P2 / Q000378:metabolism; D058473:Receptors, Purinergic P2Y2; D011985:Receptors, Serotonin / Q000378:metabolism; D018025:Receptors, Thyrotropin-Releasing Hormone / Q000378:metabolism; D017483:Receptors, Vasopressin / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D015398:Signal Transduction; D014779:Virus Replication / Q000235:genetics
D000809:Angiotensins; C546385:P2RY2 protein, human; D011941:Receptors, Adrenergic; D011966:Receptors, LHRH; D011976:Receptors, Muscarinic; D018035:Receptors, Odorant; D018048:Receptors, Purinergic P2; D058473:Receptors, Purinergic P2Y2; D011985:Receptors, Serotonin; D018025:Receptors, Thyrotropin-Releasing Hormone; D017483:Receptors, Vasopressin; D011994:Recombinant Proteins; D019206:GTP-Binding Protein alpha Subunits, Gi-Go; D020962:Heterotrimeric GTP-Binding Proteins