Longitudinal dynamics of Epstein-Barr virus-specific cytotoxic T lymphocytes during posttransplant lymphoproliferative disorder.
Epstein-Barr virus (EBV)-associated lymphoproliferative disorder (LPD) is a serious complication after allogeneic bone marrow transplantation (BMT). Dynamics of EBV-specific cytotoxic T lymphocytes (CTL), which are important in controlling EBV during the LPD, have not been fully elucidated. A patient with Wiskot-Aldrich's syndrome was diagnosed as suffering from LPD on day 47 after BMT. Fluorescence-activated cell sorter (FACS) analysis for interferon-gamma production revealed that >70% of the patient's CD8(+) T cells were EBV specific. The patient's lymphocytes were directly cytotoxic to donor-derived EBV-positive lymphoblastoid cells, which was blocked by an anti-class I antibody. EBV-specific CD8(+) T cell counts declined in parallel with EBV genome load, and full recovery of LPD was obtained with relaxation of immunosuppressive drugs. The results illustrate longitudinal dynamics of EBV-specific CTL during the posttransplant LPD; they also illustrate the advantages of using FACS analysis for EBV-specific CTL to make decisions about treatment.
Kuzushima
K
K
Division of Virology, Aichi Cancer Center Research Institute, Nagoya 464-8681, Japan. kkuzushi@aichi-c.c.pref.aichi.jp
Kimura
H
H
Hoshino
Y
Y
Yoshimi
A
A
Tsuge
I
I
Horibe
K
K
Morishima
T
T
Tsurumi
T
T
Kojima
S
S
D000954:Antigens, Surface / Q000032:analysis; D016026:Bone Marrow Transplantation / Q000009:adverse effects*; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism; D002469:Cell Separation; D004279:DNA, Viral / Q000032:analysis; D020031:Epstein-Barr Virus Infections / Q000276:immunology*; D005434:Flow Cytometry; D006650:Histocompatibility Testing; D006801:Humans; D007223:Infant; D007371:Interferon-gamma / Q000096:biosynthesis; D008137:Longitudinal Studies; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008297:Male; D016133:Polymerase Chain Reaction; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000954:Antigens, Surface; D004279:DNA, Viral; D007371:Interferon-gamma