PCR for diagnosis of paracoccidioidomycosis.
A PCR assay based on oligonucleotide primers derived from the sequence of the gene coding for the 43,000-Da (gp43) antigen was developed to detect Paracoccidioides brasiliensis DNA in sputa. In the standardized conditions, it could detect 10 cells/ml of sputum, providing sufficient accuracy to be useful for diagnosis of paracoccidioidomycosis.
Gomes
G M
GM
Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo (UNIFESP), São Paulo, SP, Brazil.
Cisalpino
P S
PS
Taborda
C P
CP
de Camargo
Z P
ZP
D000946:Antigens, Fungal / Q000235:genetics; D017931:DNA Primers; D004271:DNA, Fungal / Q000032:analysis; D005656:Fungal Proteins / Q000235:genetics*; D006023:Glycoproteins; D006801:Humans; D010228:Paracoccidioides / Q000235:genetics / Q000302:isolation & purification*; D010229:Paracoccidioidomycosis / Q000175:diagnosis* / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D013183:Sputum / Q000382:microbiology*
C103180:43 kDa protein, Paracoccidioides; D000946:Antigens, Fungal; D017931:DNA Primers; D004271:DNA, Fungal; D005656:Fungal Proteins; D006023:Glycoproteins