Smooth muscle cell adhesion to tissue engineering scaffolds.
Synthetic polyesters of lactic and glycolic acid, and the extracellular matrix molecule collagen are among the most widely-utilized scaffolding materials in tissue engineering. However, the mechanism of cell adhesion to these tissue engineering scaffolds has not been extensively studied. In this paper, the mechanism of adhesion of smooth muscle cells to these materials was investigated. Vitronectin was found to be the predominant matrix protein adsorbed from serum-containing medium onto polyglycolic acid, poly(lactic co-glycolic) acid, and collagen two-dimensional films and three-dimensional scaffolds. Fibronectin adsorbed to both materials as well, although to a much lower density. Smooth muscle cell adhesion was mediated through specific integrin receptors interacting with these adsorbed proteins, as evidenced by both immunostaining and blocking studies. The receptors involved in adhesion included the alpha(v)beta5 to vitronectin, the alpha5beta1 to fibronectin and the alpha2beta1 to collagen I. Identification of the specific receptors used to adhere to these polymers clarifies why smooth muscle tissue development differs on these scaffolds, and may allow one to design tissue formation by controlling the surface chemistry of tissue engineering scaffolds.
Nikolovski
J
J
Department of Biomedical Engineering, Dental School, University of Michigan, Ann Arbor, MI 48109, USA. janeta@umich.edu
Mooney
D J
DJ
D000327:Adsorption; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001672:Biocompatible Materials; D001798:Blood Proteins / Q000378:metabolism; D002448:Cell Adhesion / Q000276:immunology; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D016023:Integrins / Q000276:immunology; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000378:metabolism; D051381:Rats
D000911:Antibodies, Monoclonal; D001672:Biocompatible Materials; D001798:Blood Proteins; D016326:Extracellular Matrix Proteins; D016023:Integrins