title: The effects of cryopreservation on semen from men with sarcoma or carcinoma.
abstract: OBJECTIVE
  This study evaluated prefreeze and postthaw semen quality before treatment in patients with different types of sarcomas and carcinomas to determine whether cryopreservation would be of value for these patients.
  
  
  METHODS
  Semen specimens were obtained from 50 normal donors and from 21 patients with carcinoma and from 14 patients with sarcoma. The specimens were cryopreserved by a standard freezing procedure using TEST-Yolk buffer. Prefreeze and postthaw sperm motion characteristics were measured.
  
  
  RESULTS
  Prefreeze total motile sperm count was significantly higher in donors (median: 129.6 x 10(6)/ml) than in men with carcinoma (46.9 x 10(6)/ml, P < .001) or sarcoma (66.3 10(6)/ml, P = .04). The percent motility and percent linearity were significantly lower in patients with carcinoma. In postthaw specimens, total motile sperm count, curvilinear velocity, and linearity were significantly lower in patients with carcinoma.
  
  
  CONCLUSIONS
  The two patient groups in this study had poor semen quality when compared with healthy donors both before and after cryopreservation. Sarcoma patients had better semen quality than carcinoma patients. As cancer therapy in these men could significantly impair their reproductive potential, these men should be advised to preserve their semen before starting treatment.
authors.affiliation: Center For Advanced Research in Human Reproduction and Infertility, Cleveland Clinic Foundation, Ohio 44195, USA.
authors.forename: J
authors.initials: J
authors.lastname: Hallak
authors.forename: A
authors.initials: A
authors.lastname: Mahran
authors.forename: J
authors.initials: J
authors.lastname: Chae
authors.forename: A
authors.initials: A
authors.lastname: Agarwal
mesh_terms: D000328:Adult; D002277:Carcinoma / Q000150:complications*; D015925:Cryopreservation; D006801:Humans; D007248:Infertility, Male / Q000209:etiology / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D012509:Sarcoma / Q000150:complications*; D012661:Semen; D012662:Semen Preservation; D013074:Sperm Banks; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000473:pathology / Q000502:physiology