Sequence analysis and deletion of porcine adenovirus serotype 5 E3 region.
A 3000 basepair (bp) region corresponding to the E3 region, the flanking pVIII and part of the fiber protein genes, of the two prototype strains (HNF-61 and HNF-70) of porcine adenovirus serotype five (PAdV-5) was sequenced. A potential E3 promoter and poly-A signals were identified. The size of the E3 region was 2039 (strain HNF-61) and 2020 bp (strain HNF-70) the largest E3 so far reported among PAdVs. Three open reading frames (ORF2-4) were identified within the E3 region. Based on the predicted amino acid (aa) sequences ORF2 was similar to other adenovirus E3 ORFs, ORF3 showed some similarity to a bovine adenovirus (BAdV-1) ORF. ORF4 was unique to PAdV-5. E3 mRNA transcripts were detected early in infection by Northern blot analysis. Genomic clones of HNF-70 with a 1505 or 1237 bp deletions in the E3 region were constructed to map non-essential regions. After transfection of the DNA into swine testicle cells, virions were recovered for only the shorter 1237 bp deletion. At least 60% of the E3 region was not essential for virus replication, bringing the theoretical vector capacity of a helper independent PAdV-5 to 2.9 kb.
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Department of Pathobiology, University of Guelph, Ont., N1G 2W1, Guelph, Canada.
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D017503:Adenovirus E3 Proteins / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D002417:Cattle; D004261:DNA Replication; D004279:DNA, Viral; D017921:Mastadenovirus / Q000235:genetics*; D008969:Molecular Sequence Data; D017421:Sequence Analysis / Q000379:methods; D017386:Sequence Homology, Amino Acid; D013552:Swine; D014158:Transcription, Genetic; D014779:Virus Replication
D017503:Adenovirus E3 Proteins; D036022:Capsid Proteins; D004279:DNA, Viral; C018315:hexon capsid protein, Adenovirus