title: Release of monocyte chemoattractant protein (MCP)-1 by a human alveolar epithelial cell line in response to mycobacterium avium.
abstract: Clinical strains of Mycobacterium avium isolated from patients with acquired immunodeficiency syndrome, but not a non-clinical laboratory strain (ATCC 25291), were found to stimulate the human alveolar epithelial cell line A549, to produce monocyte chemoattractant protein (MCP)-1. A549 cells were also found to produce elevated levels of MCP-1 in response to sonicates of the clinical strains of M. avium, and surprisingly, the non-clinical strain as well. However, sonic extracts of the clinical strains were found to induce significantly higher levels of MCP-1 production compared to extracts of the non-clinical strain (P < 0.001). These data suggest the existence of strain-related differences in antigen expression by M. avium. The clinical and non-clinical strains of M. avium were found to attach and invade, but not replicate in A549 cells indicating that MCP-1 production by A549 cells does require the presence of viable, replicating organisms. Activation of alveolar epithelial cells by exposure to M. avium resulting in the production of chemokines which recruit inflammatory cells to the site of infection may be an important regulatory pathway for the activation of pulmonary host defense.
authors.affiliation: Department of Medicine, University of California, San Diego 92103-8374, USA. srao@ucsd.edu
authors.forename: S P
authors.initials: SP
authors.lastname: Rao
authors.forename: T
authors.initials: T
authors.lastname: Hayashi
authors.forename: A
authors.initials: A
authors.lastname: Catanzaro
mesh_terms: D018932:Chemokine CCL2 / Q000378:metabolism*; D006801:Humans; D015269:Mycobacterium avium Complex / Q000254:growth & development / Q000276:immunology*; D011650:Pulmonary Alveoli / Q000276:immunology / Q000382:microbiology; D020545:Respiratory Mucosa / Q000276:immunology* / Q000382:microbiology*; D013010:Sonication; D014407:Tumor Cells, Cultured
chemical_list: D018932:Chemokine CCL2