title: Nucleotide sequence of the structural protein-encoding region of foot-and-mouth disease virus A22-India.
abstract: Nucleotide sequence of the structural protein-encoding region of foot-and-mouth disease virus (FMDV) A22-India 17/77 was determined using non-radioisotopic technique. Comparison of nucleotide and deduced amino acid sequence with A22-Iraq 24/64 revealed 175 synonymous (silent) and 42 non-synonymous nucleotide changes resulting in 34 amino acid substitutions along the capsid proteins (VP1-VP4). Out of the 4 structural proteins VP4 is highly conserved. The highly variable and immunodominant protein VP1 showed 47% of the total amino acid substitutions. VP2 and VP3 contain 38.2% and 14.7% of the amino acid substitutions, respectively. The VP1-based phylogenetic analysis of 18 different type A viruses including A22-India 17/77 divided them in to two broad genetic groups (Asian and European/South American), and each group is further subdivided in to two separate genotypes. A22-India 17/77, A22-Iraq 24/64 and A22-Azerbaijan/65 formed one genotype and the 4 Chinese strains formed a separate genotype in the Asian group of viruses. In the European/South American group, A-Argentina/87 represents one genotype and the remaining 10 strains formed the second genotype in this group.
authors.affiliation: Central Laboratory, All India Coordinated Research Project on Foot-and-Mouth Disease, Indian Veterinary Research Institute, Mukteswar-Kumaon, Nainital.
authors.forename: C
authors.initials: C
authors.lastname: Tosh
authors.forename: R
authors.initials: R
authors.lastname: Venkataramanan
authors.forename: D
authors.initials: D
authors.lastname: Hemadri
authors.forename: A
authors.initials: A
authors.lastname: Sanyal
authors.forename: A R
authors.initials: AR
authors.lastname: Samuel
authors.forename: N J
authors.initials: NJ
authors.lastname: Knowles
authors.forename: R P
authors.initials: RP
authors.lastname: Kitching
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005537:Aphthovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D015678:Viral Structural Proteins / Q000032:analysis / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D015678:Viral Structural Proteins