title: Partitioning of whey proteins, bovine serum albumin and porcine insulin in aqueous two-phase systems.
abstract: Partitioning of the proteins from cheese whey, bovine serum albumin and porcine insulin were analysed using aqueous two-phase systems (ATPS) prepared with PEG-phosphate, PEG-citrate and PEG-maltodextrin (MD). Proteins were quantified through one of the following methods: FPLC, Bradford and spectrophotometry at 280 nm. Results showed that whey proteins partitioned unevenly on the phases of the systems used, with alpha-lactoalbumin (alpha-La) concentrated in the upper phase and beta-lactoglobulin (beta-Lg) in the lower. Albumin in PEG-MD systems concentrated in the MD-rich lower phase. Porcine insulin showed great affinity with the PEG-rich phase, its partition coefficient was always over 10 and increases with PEG molecular mass.
authors.affiliation: Food Engineering Department, FEA, State University of Campinas, SP, Brazil.
authors.forename: J G
authors.initials: JG
authors.lastname: Alves
authors.forename: L D
authors.initials: LD
authors.lastname: Chumpitaz
authors.forename: L H
authors.initials: LH
authors.lastname: da Silva
authors.forename: T T
authors.initials: TT
authors.lastname: Franco
authors.forename: A J
authors.initials: AJ
authors.lastname: Meirelles
mesh_terms: D000818:Animals; D002417:Cattle; D002951:Citrates / Q000737:chemistry; D007328:Insulin / Q000302:isolation & purification*; D008894:Milk Proteins / Q000302:isolation & purification*; D011092:Polyethylene Glycols / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000302:isolation & purification*; D000077559:Sodium Citrate; D013552:Swine; D000067816:Whey Proteins
chemical_list: D002951:Citrates; D007328:Insulin; D008894:Milk Proteins; D000067816:Whey Proteins; D000077559:Sodium Citrate; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols