Expression of a tetraheme protein, Desulfovibrio vulgaris Miyazaki F cytochrome c(3), in Shewanella oneidensis MR-1.
Cytochrome c(3) from Desulfovibrio vulgaris Miyazaki F was successfully expressed in the facultative aerobe Shewanella oneidensis MR-1 under anaerobic, microaerophilic, and aerobic conditions, with yields of 0.3 to 0.5 mg of cytochrome/g of cells. A derivative of the broad-host-range plasmid pRK415 containing the cytochrome c(3) gene from D. vulgaris Miyazaki F was used for transformation of S. oneidensis MR-1, resulting in the production of protein product that was indistinguishable from that produced by D. vulgaris Miyazaki F, except for the presence of one extra alanine residue at the N terminus.
Ozawa
K
K
Department of Chemistry and Biotechnology, Faculty of Engineering, Yokohama National University, Hodogaya-ku, Yokohama 240-8501, Japan.
Tsapin
A I
AI
Nealson
K H
KH
Cusanovich
M A
MA
Akutsu
H
H
D000332:Aerobiosis; D000693:Anaerobiosis; D003574:Cytochrome c Group / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D016969:Desulfovibrio vulgaris / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D020592:Shewanella / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
D003574:Cytochrome c Group; D011994:Recombinant Proteins; C017165:cytochrome c(3)