Rat multispecific organic anion transporter 1 (rOAT1) transports zidovudine, acyclovir, and other antiviral nucleoside analogs.
Organic anion transporter 1 (OAT1) is a p-aminohippurate/dicarboxylate exchanger that plays a primary role in the tubular secretion of endogenous and exogenous organic anions. OAT1 is located in the basolateral membrane of the proximal tubular cells and mediates the uptake of various organic anions from the peritubular fluid. In this study, we investigated the transport of antiviral nucleoside analogs via rat OAT1 (rOAT1) using a heterologous expression system in Xenopus laevis oocytes. Oocytes injected with rOAT1 cRNA showed significantly higher uptake of zidovudine (AZT) and acyclovir (ACV) than control oocytes. rOAT1-mediated uptake of AZT and ACV was probenecid-sensitive and increased by the outwardly directed gradient of glutarate. The affinity of rOAT1 for AZT and ACV was determined to be 68 and 242 microM, respectively. Five other antiviral agents that we studied (zalcitabine, didanosine, lamivudine, stavudine, and trifluridine) were also shown to be transported by rOAT1, whereas foscarnet, a phosphate analog, was not. The aforementioned nucleoside analogs lack a typical anionic group and are not very hydrophobic. This study demonstrates extension of the substrate spectrum of rOAT1 and provides a molecular basis for the pharmacokinetics of antiviral nucleoside analogs.
Wada
S
S
Department of Pharmacology and Toxicology, Kyorin University School of Medicine, Mitaka, Tokyo, Japan.
Tsuda
M
M
Sekine
T
T
Cha
S H
SH
Kimura
M
M
Kanai
Y
Y
Endou
H
H
D000212:Acyclovir / Q000378:metabolism*; D000818:Animals; D027321:Anion Transport Proteins; D000998:Antiviral Agents / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D016049:Didanosine / Q000378:metabolism; D066298:In Vitro Techniques; D019259:Lamivudine / Q000378:metabolism; D009865:Oocytes / Q000378:metabolism; D051381:Rats; D018119:Stavudine / Q000378:metabolism; D014162:Transfection; D014271:Trifluridine / Q000378:metabolism; D014982:Xenopus laevis; D016047:Zalcitabine / Q000378:metabolism; D015215:Zidovudine / Q000378:metabolism*
D027321:Anion Transport Proteins; D000998:Antiviral Agents; D002352:Carrier Proteins; D019259:Lamivudine; D015215:Zidovudine; D016047:Zalcitabine; D018119:Stavudine; D016049:Didanosine; D014271:Trifluridine; D000212:Acyclovir