Combined effect of titanium particles and TNF-alpha on the production of IL-6 by osteoblast-like cells.
To clarify the role of tumor necrosis factor (TNF)-alpha on osteoblast functions in the presence of metal particles, two human osteoblast-like cell lines (MG-63 and SaOS-2) were cultured with TNF-alpha in the presence or absence of titanium particles in vitro. A combination of TNF-alpha and titanium particles showed additive effects on inhibition of cell proliferation and alkaline phosphatase production. On the other hand, production of interleukin-6, which is well known to induce osteoclastogenesis and to directly stimulate bone resorption, was additively stimulated by the combination of TNF-alpha and titanium particles. These results suggest that the association of TNF-alpha and titanium particles may play an important role in the pathogenesis of periprosthetic osteolysis through two different pathways: a reduced periprosthetic bone formation due to inhibition of osteoblast proliferation and alkaline phosphatase production, and osteoblast-mediated activation of osteoclastic bone resorption as suggested by the enhancement of interleukin-6 production.
Takei
H
H
Department of Orthopaedics, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093-0412, USA.
Pioletti
D P
DP
Kwon
S Y
SY
Sung
K L
KL
D001672:Biocompatible Materials / Q000009:adverse effects; D002460:Cell Line; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis*; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism*; D014025:Titanium / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D001672:Biocompatible Materials; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D014025:Titanium