Differential gene expression in nasopharyngeal carcinoma cells.
Nasopharyngeal carcinoma (NPC) is a common cancer among southern Chinese. The profile of gene expression in NPC cells is largely unknown. In this study, we have examined differential gene expression in non-malignant and malignant nasopharyngeal epithelial (NPE) cells using a cDNA array hybridization method. A total of 42 genes were identified to be expressed in either non-malignant and malignant NPE cells or both. Thirteen of these genes were overexpressed in malignant NPE cells. These includes nuclear factor (NF90), FOS-related antigen 1 (FRA- 1), cytoplasmic dynein light chain (HDLC1), replication factor C (RFC1), nucleoside diphosphate kinase B, UV excision repair protein (RAD23A), insulin-like growth factor receptor II, transcription initiation factor TFIID subunit (TAFII31), growth factor receptor-bound protein 2 (GRB2), UV excision repair protein (RAD23B), glutathione peroxidase, Y box binding protein 1 and heat shock protein 86. In contrast, expression of nine genes was suppressed in malignant NPE cells. These includes calgranulin A, calgranulin B, neutrophil activating protein (ENA-78), heat shock protein 27, integrin beta-1, integrin beta-4, cyclin-dependent kinase inhibitor 1A (p21), interleukin-8 and tyrosine protein kinase receptor (RET). Differential expression of calgranulin A, calgraunlin B, ENA-78, FRA-1 and NF90 in non-malignant and malignant nasopharyngeal epithelial cells was confirmed by RT-PCR analysis.
Fung
L F
LF
Dept of Anatomy, Faculty of Medicine, University of Hong Kong, SAR, People's Republic of China.
Lo
A K
AK
Yuen
P W
PW
Liu
Y
Y
Wang
X H
XH
Tsao
S W
SW
D002135:Calcium-Binding Proteins / Q000235:genetics; D040501:Calgranulin A; D040502:Calgranulin B; D054365:Chemokine CXCL5; D019743:Chemokines, CXC; D018076:DNA, Complementary / Q000032:analysis; D004268:DNA-Binding Proteins / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016209:Interleukin-8 / Q000031:analogs & derivatives / Q000235:genetics; D050778:NFATC Transcription Factors; D009303:Nasopharyngeal Neoplasms / Q000235:genetics*; D009305:Nasopharynx / Q000378:metabolism; D050779:Nuclear Factor 90 Proteins; D009687:Nuclear Proteins; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics; D014407:Tumor Cells, Cultured; D000099169:Fos-Related Antigen 1
D002135:Calcium-Binding Proteins; D040501:Calgranulin A; D040502:Calgranulin B; D054365:Chemokine CXCL5; D019743:Chemokines, CXC; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D016209:Interleukin-8; D050778:NFATC Transcription Factors; D050779:Nuclear Factor 90 Proteins; D009687:Nuclear Proteins; D016760:Proto-Oncogene Proteins c-fos; D014157:Transcription Factors; D000099169:Fos-Related Antigen 1; C486263:CXCL5 protein, human