title: Biogenesis of the protein storage vacuole crystalloid.
abstract: We identify new organelles associated with the vacuolar system in plant cells. These organelles are defined biochemically by their internal content of three integral membrane proteins: a chimeric reporter protein that moves there directly from the ER; a specific tonoplast intrinsic protein; and a novel receptor-like RING-H2 protein that traffics through the Golgi apparatus. Highly conserved homologues of the latter are expressed in animal cells. In a developmentally regulated manner, the organelles are taken up into vacuoles where, in seed protein storage vacuoles, they form a membrane-containing crystalloid. The uptake and preservation of the contents of these organelles in vacuoles represents a unique mechanism for compartmentalization of protein and lipid for storage.
authors.affiliation: Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340, USA.
authors.forename: L
authors.initials: L
authors.lastname: Jiang
authors.forename: T E
authors.initials: TE
authors.lastname: Phillips
authors.forename: S W
authors.initials: SW
authors.lastname: Rogers
authors.forename: J C
authors.initials: JC
authors.lastname: Rogers
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000502:physiology; D017124:Conserved Sequence; D019662:Cucurbitaceae / Q000502:physiology; D004721:Endoplasmic Reticulum / Q000502:physiology; D006056:Golgi Apparatus / Q000502:physiology; D008969:Molecular Sequence Data; D015388:Organelles / Q000502:physiology* / Q000648:ultrastructure; D010940:Plant Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D010944:Plants / Q000648:ultrastructure*; D030821:Plants, Genetically Modified; D011993:Recombinant Fusion Proteins / Q000032:analysis; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014617:Vacuoles / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D010940:Plant Proteins; D011993:Recombinant Fusion Proteins