Sequential analysis of adhesion molecules and their ligands in rat renal allografts during the development of chronic rejection.
Intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) are important in endothelial cell-leukocyte interactions. In this sequential study, the expression of ICAM-1 and VCAM-1 and their ligands LFA-1 and VLA-4 as well as major histocompatibility complex class II antigens (MHC class II), and interleukin-2-receptor (IL-2R) were investigated during the development of chronic renal allograft rejection in a rat model. The time-related expression of adhesion molecules and their ligands in the graft was correlated to the chronic allograft damage index (CADI). In association with an initial short immune activation, there was a significant ICAM-1 and VCAM-1 induction in the vascular endothelium and the tubular epithelium. In the interstitium, there was infiltration of lymphocytes expressing ligand molecules VLA-4 and LFA-1, as well as activation markers MHC class II and IL-2R. Thereafter, the expression declined together with the increase of CADI-values. In end-stage chronic rejection, there was practically no expression of ICAM-1 and VCAM-1. In the interstitium, there were only few ligand-expressing leukocytes. In conclusion, adhesion molecules and their ligands are involved in the induction phase of the process but no longer in the later stages of chronic rejection.
Kauppinen
H
H
Fourth Department of Surgery, Helsinki University Central Hospital, Finland.
Soots
A
A
Krogerus
L
L
Loginov
R
R
Holma
K
K
Ahonen
J
J
Lautenschlager
I
I
D000818:Animals; D015815:Cell Adhesion Molecules / Q000032:analysis* / Q000378:metabolism*; D002908:Chronic Disease; D006084:Graft Rejection / Q000378:metabolism* / Q000473:pathology*; D007150:Immunohistochemistry; D039041:Integrin alpha4beta1; D016023:Integrins / Q000032:analysis / Q000378:metabolism; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis / Q000378:metabolism; D016030:Kidney Transplantation / Q000473:pathology*; D008024:Ligands; D016169:Lymphocyte Function-Associated Antigen-1 / Q000032:analysis / Q000378:metabolism; D008297:Male; D051381:Rats; D011914:Rats, Inbred BN; D011919:Rats, Inbred Strains; D016201:Receptors, Lymphocyte Homing / Q000032:analysis / Q000378:metabolism; D013194:Staining and Labeling; D014184:Transplantation, Homologous; D019010:Vascular Cell Adhesion Molecule-1 / Q000032:analysis / Q000378:metabolism
D015815:Cell Adhesion Molecules; D039041:Integrin alpha4beta1; D016023:Integrins; D008024:Ligands; D016169:Lymphocyte Function-Associated Antigen-1; D016201:Receptors, Lymphocyte Homing; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1