Genomic construct and mapping of the gene for CMAP (leukocystatin/cystatin F, CST7) and identification of a proximal novel gene, BSCv (C20orf3).
It is proposed that CMAP (leukocystatin/cystatin F, HGMW-approved symbol CST7) expression is correlated with the metastatic potential of malignant tumors. FISH analysis of human and murine CMAP revealed the genomic loci 20p11.21-p11.22 of the human family 2 cystatin cluster and mouse chromosome region 2G1-G3, respectively. Like murine CMAP, the human CMAP gene is constructed from four divided exons, all of which encode the functional domains of the putative translational product. Based on the computational analysis, a novel gene weakly similar to the plant strictosidine synthase, named BSCv (HGMW-approved symbol C20orf3), was identified on the opposite allele at a distance of a few kilobases from the human CMAP gene. In between human CMAP and the BSCv gene, there is a unique tandem repeat sequence. CpG-rich island characteristics and GC-box features normally observed in housekeeping genes were not seen around exon 1 of the CMAP gene, reflecting the restricted expression of CMAP in hematopoietic cells.
Morita
M
M
Banyu Tsukuba Research Institute in collaboration with Merck Research Laboratories, Tsukuba, Ibaraki, Japan.
Hara
Y
Y
Tamai
Y
Y
Arakawa
H
H
Nishimura
S
S
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D014408:Biomarkers, Tumor; D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2; D019295:Computational Biology; D015891:Cystatins / Q000235:genetics*; D017931:DNA Primers / Q000737:chemistry; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics*; D005091:Exons; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D020080:Tandem Repeat Sequences
D014408:Biomarkers, Tumor; C116891:CST7 protein, human; D015891:Cystatins; D017931:DNA Primers; D004273:DNA, Neoplasm; C550521:cystatin F, mouse