Conditions required for a hybrid artificial liver support system using a PUF/hepatocyte-spheroid packed-bed module and it's use in dogs with liver failure.
We studied the effects of a hybrid artificial liver support system we developed on dogs with hepatic failure. The system consisted of a multi-channel polyurethane foam packed-bed culture module, including primary dog hepatocyte spheroids. Blood ammonia was well metabolized by 20 g hepatocytes, but the other functions such as glucose concentration, total bile acid concentration, and survival time required 30 g hepatocytes to improve conditions. We found that we should use a culture substratum that easily forms spheroids, and that an artificial liver module should be used as soon as possible after spheroid formation by hepatocytes in the module.
Ijima
H
H
Department of Chemical Systems and Engineering, Graduate School of Engineering, Kyushu University, Fukuoka, Japan.
Nakazawa
K
K
Koyama
S
S
Kaneko
M
M
Matsushita
T
T
Gion
T
T
Shirabe
K
K
Shimada
M
M
Takenaka
K
K
Sugimachi
K
K
Funatsu
K
K
D000818:Animals; D002478:Cells, Cultured; D004285:Dogs; D005260:Female; D022781:Hepatocytes; D017093:Liver Failure / Q000628:therapy*; D019164:Liver, Artificial; D008297:Male; D011140:Polyurethanes
D011140:Polyurethanes; C028279:polyurethane foam