Ultrastructural analysis of v-myb oncogene product cooperation with components of avian cell nuclear matrix.
The cooperation of the v-Myb oncoprotein with extracted nuclear matrix of avian haematopoietic cells expressing the v-myb oncogene was studied by means of immunoelectron microscopy. The nuclear matrix was extracted by a gentle method of detergent treatment at moderate ionic strength and visualized either in ultrathin LR White sections, in unembedded resin-free sections, and in addition by the aqueous spreading technique. Using anti-Myb polyclonal antibody we have shown interaction of the v-Myb protein product with extracted nuclear matrix. This oncoprotein, however, was easily released from the structure by a detergent as well as by DNAase treatment and ammonium sulphate extraction. Prefixation of structures before detergent treatment prevented this extraction. The v-Myb protein marker was distributed in clusters or associated with fibrillar structures in most cases. Single markers decorating these fibrillar or less dense structures were also detected.
Korb
J
J
Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague.
Stokrová
J
J
Karafiát
V
V
D000818:Animals; D001717:Birds; D015153:Blotting, Western; D002458:Cell Fractionation; D002460:Cell Line; D003902:Detergents; D006413:Hematopoietic System / Q000166:cytology; D016253:Microscopy, Immunoelectron / Q000379:methods*; D015530:Nuclear Matrix / Q000378:metabolism* / Q000648:ultrastructure*; D020626:Oncogene Proteins v-myb / Q000378:metabolism*
D003902:Detergents; D020626:Oncogene Proteins v-myb