A new method for one-step, no-carrier-added synthesis of cholesteryl 4-[18F]-fluorobenzoate ([18F]-CFB), a radiotracer used in detection of adrenal malignancies.
OBJECTIVE
Cholesteryl 4-[(18)F]-fluorobenzoate, a potential radiotracer used for adrenal and ovarian imaging, was prepared in no-carrier-added form from cholesteryl 4-N,N,N-trimethylanilinium trifluoromethanesulfonate.


METHODS
The reaction was performed in one step using Kryptofix2.2.2/[(18)F], carbonate as the counter ion and dimethyl sulfoxide as the solvent at 110 degrees C. Purification was performed using commercially available C(18) and Si Sep-Paks.


RESULTS
Column purification afforded the desired compound in 75-85 % radiochemical yield (EOS) with a specific activity about 74 KBq/mmole in about 20 minutes, with greater than 95% radiochemical and chemical purity (HPLC and TLC analysis).


CONCLUSIONS
This compound was prepared through a novel method which can be easily performed at distant locations from the main radionuclide production centers using Sep-Paks. The biodistribution of this compound in mice was confirmed to be similar to that reported in the literature.
Jalilian
A R
AR
Cyclotron Department, Nuclear Research Center for Agriculture & Medicine (NRCAM), Atomic Energy Organization of Iran (AEOI), P.O. Box: 31585-4395, Karaj, Iran.
Afarideh
H
H
Najafi
R
R
Shafiee
A
A
Bineshmarvasti
M
M
D000310:Adrenal Gland Neoplasms / Q000000981:diagnostic imaging; D000311:Adrenal Glands / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D001565:Benzoates / Q000138:chemical synthesis* / Q000737:chemistry / Q000493:pharmacokinetics; D002788:Cholesterol Esters / Q000138:chemical synthesis* / Q000737:chemistry / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004997:Ethinyl Estradiol / Q000494:pharmacology; D005260:Female; D005462:Fluorine Radioisotopes / Q000737:chemistry*; D007553:Isotope Labeling / Q000379:methods; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008815:Mice, Inbred Strains; D010053:Ovary / Q000378:metabolism; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000138:chemical synthesis* / Q000737:chemistry / Q000493:pharmacokinetics; D013055:Spectrophotometry, Infrared; D014018:Tissue Distribution
D001565:Benzoates; D002788:Cholesterol Esters; D005462:Fluorine Radioisotopes; D019275:Radiopharmaceuticals; C120325:cholesteryl 4-fluorobenzoate; D004997:Ethinyl Estradiol