Assessment of the effect of Maytenus ilicifolia (espinheira santa) extract on the labeling of red blood cells and plasma proteins with technetium-99m.
We are trying to develop a model to assess properties of products utilized in popular medicine. Maytenus ilicifolia is used in herbal medicine. Red blood cells (RBC) labeled with technetium-99m (99mTc) are employed in nuclear medicine. This labeling procedure depends on a reducing agent and stannous chloride is used. There is evidence that this labeling may be altered by drugs. We have investigated the possibility of M. ilicifolia extract being capable to alter the labeling of blood elements with 99mTc. Blood was incubated with M. ilicifolia extract. Stannous chloride solution and Tc-99m were added. Blood was centrifuged and plasma (P) and blood cells (C) were isolated. Samples of P or C were also precipitated, centrifuged and insoluble (IF) and soluble (SF) were separated. The percentages of radioactivity (%ATI) in C, IF-P and IF-C was calculated. The %ATI decreased on C from 93.6+/-2.3 to 29.0+/-2.7, on IF-P from 77.6+/-1.2 to 7.5+/-1.0 and on IF-C from 80.0+/-3.4 to 12.6+/-4.8. Once in RBC labeling procedure with 99mTc depends on the presence of stannous (+2) ions, the substances of the M. ilicifolia extract could increase the valence these ions to stannic (+4). This fact would decrease the %ATI on blood elements and indicate the presence of oxidant agents in the M. ilicifolia extract.
de Oliveira
J F
JF
Universidade do Estado do Rio de Janeiro, Instituto de Biologia Roberto Alcantara Gomes, Departamento de Biofísica e Biometria. Av. 28 de Setembro, 87, Rio de Janeiro, RJ 20551-030, Brazil.
Braga
A C
AC
de Oliveira
M B
MB
Avila
A S
AS
Caldeira-de-Araújo
A
A
Cardoso
V N
VN
Bezerra
R J
RJ
Bernardo-Filho
M
M
D001798:Blood Proteins / Q000737:chemistry*; D001938:Brazil; D004912:Erythrocytes / Q000187:drug effects* / Q000648:ultrastructure; D006801:Humans; D007553:Isotope Labeling; D016877:Oxidants / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D013667:Technetium; D017971:Tin Compounds / Q000097:blood
D001798:Blood Proteins; D016877:Oxidants; D017971:Tin Compounds; C023599:stannous chloride; D013667:Technetium