Current applications of single-cell PCR.
The advent of the polymerase chain reaction (PCR) has revolutionised the way in which molecular biologists view their task at hand, for it is now possible to amplify and examine minute quantities of rare genetic material: the limit of this exploration being the single cell. It is especially in the field of prenatal diagnostics that this ability has been readily seized upon, as it has opened up the prospect of preimplantation genetic analysis and the use of fetal cells enriched from the blood of pregnant women for the assessment of single-gene Mendelian disorders. However, apart from diagnostic applications, single-cell PCR has proven to be of enormous use to basic scientists, addressing diverse immunological, neurological and developmental questions, where both the genome but also messenger RNA expression patterns were examined. Furthermore, recent advances, such as optimised whole genome amplification (WGA) procedures, single-cell complementary DNA arrays and perhaps even single-cell comparative genomic hybridisation will ensure that the genetic analysis of single cells will become common practice, thereby opening up new possibilities for diagnosis and research.
Hahn
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Department of Obstetrics and Gynaecology, University of Basel, Switzerland. shahn@uhbs.ch
Zhong
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Troeger
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Burgemeister
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Gloning
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Holzgreve
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D000818:Animals; D005312:Fetal Blood / Q000166:cytology / Q000378:metabolism; D005333:Fetus / Q000166:cytology* / Q000378:metabolism* / Q000473:pathology; D015321:Gene Rearrangement / Q000235:genetics; D030342:Genetic Diseases, Inborn / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D006801:Humans; D007834:Lasers; D008846:Micromanipulation / Q000379:methods; D018895:Microsatellite Repeats / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D011296:Prenatal Diagnosis / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods