Seeing is believing: recent trends in the measurement of Ca2+ in subcellular domains and intracellular organelles.
The role of Ca2+ in the regulation of the cell cycle has been investigated mostly in studies assessing global cytosolic free Ca2+. Recent studies, however, have used unique techniques to assess Ca2+ in subcellular organelles, such as mitochondria, and in discrete regions of the cytoplasm. These studies have used advanced fluorescence digital imaging techniques and Ca2+-sensitive fluorescence probes, and/or targeting of Ca2+-sensitive proteins to intracellular organelles. The present review describes the results of some of these studies and the techniques used. The novel techniques used to measure Ca2+ in microdomains and intracellular organelles are likely to be of great use in future investigations assessing Ca2+ homeostasis during the cell cycle.
Monteith
G R
GR
School of Pharmacy, The University of Queensland, St Lucia, Australia. greg@pharmacy.uq.edu.au
D000818:Animals; D002118:Calcium / Q000032:analysis* / Q000378:metabolism; D015220:Calcium Channels / Q000378:metabolism; D002453:Cell Cycle; D003593:Cytoplasm / Q000737:chemistry*; D006706:Homeostasis; D008856:Microscopy, Fluorescence; D015388:Organelles / Q000737:chemistry*
D015220:Calcium Channels; D002118:Calcium