Vitamin E level changes in serum and red blood cells due to acute exhaustive exercise in collegiate women.
The purpose of this study was to investigate the change in vitamin E level in both serum and red blood cells (RBC) during exercise and to clarify the effect of vitamin E supplementation. Ten young sedentary female subjects received 200 mg D-alpha-tocopherol acetate daily for 1 wk after the initial exercise bout. After 1 wk of vitamin E supplementation, the same subjects repeated the same exercise. Before vitamin E supplementation, the alpha-tocopherol level in the serum (serum-alpha-tocopherol) did not change after exercise, but a significant decrease in the alpha-tocopherol level in RBC (RBC-alpha-tocopherol) was observed after exercise (p < 0.05). On the other hand, after vitamin E supplementation, the serum-alpha-tocopherol level decreased significantly after exercise (p < 0.05), while the RBC-alpha-tocopherol level was maintained after exercise. Furthermore, a negative correlation between the changes in serum- and RBC-alpha-tocopherol levels was observed only after vitamin E supplementation (r = 0.667, p < 0.05). The present results suggest that as RBC suffers oxidative stress, vitamin E in RBC is consumed to protect RBC from oxidative damage during exercise. These results also suggest that when there is a sufficient amount of vitamin E in the serum, vitamin E is shifted from the serum to RBC, resulting in a steady RBC-alpha-tocopherol level and a decrease in the serum-alpha-tocopherol level under oxidative stress such as exercise.
Kawai
Y
Y
Department of Preventive Medicine and Public Health, Tokyo Medical University, Japan. kawai-y@tokyo.med.ac.jp
Shimomitsu
T
T
Takanami
Y
Y
Murase
N
N
Katsumura
T
T
Maruyama
C
C
D000328:Adult; D004041:Dietary Fats / Q000008:administration & dosage; D019587:Dietary Supplements; D004912:Erythrocytes / Q000378:metabolism*; D015444:Exercise / Q000502:physiology*; D005260:Female; D006801:Humans; D018384:Oxidative Stress; D013334:Students; D014810:Vitamin E / Q000097:blood* / Q000494:pharmacology
D004041:Dietary Fats; D014810:Vitamin E