[Effects of ischemic preconditioning on apoptosis of hepatocytes in liver transplantation in rats].
OBJECTIVE
To evaluate the effects of ischemic preconditioning (IP) on hepatocyte apoptosis in ischemia/reperfusion injury of grafted liver and clarify its mechanism.


METHODS
Orthotopic liver transplantation with cuff anastomosing method for the portal vein, suprahepatic vena cava and infrahepatic vena cava in the rats were divided into two groups: with and without IP treatment. The parameters of liver function were determined by auto-biochemical analyser. The concentrations of malondialdehyde (MDA) were measured with chromometry. The apoptotic hepatocytes in grafts liver were detected by flow cytometry combined with the in situ terminal deoxynucleotidyl transferase-mediated dUTP-FITC nick end-labeling (TUNEL) method.


RESULTS
The levels of AST, ALT, LDH in the serum, and MDA and apoptotic cells in the liver were significantly increased compared with normal values after ischemia/reperfusion to the grafted liver. After IP treatment, the levels of AST, ALT, LDH, MDA and apoptotic cells were significantly decreased.


CONCLUSIONS
IP can attenuate ischemia/reperfusion injury by alleviating lipid peroxide and decreasing apoptosis of hepatocytes.
Zhang
X
X
Department of General Surgery, Renji Hospital, Shanghai Second Medical University, Shanghai 200001, China.
Cao
H
H
Jiao
Z
Z
Ling
W
W
Wu
Z
Z
Chen
Z
Z
Kuang
Y
Y
D000818:Animals; D017209:Apoptosis; D007511:Ischemia / Q000378:metabolism / Q000473:pathology*; D019194:Ischemic Preconditioning; D015227:Lipid Peroxidation; D008099:Liver / Q000098:blood supply / Q000473:pathology*; D016031:Liver Transplantation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000517:prevention & control*