Halotolerance of the yeast Arxula adeninivorans LS3.
The non-pathogenic, dimorphic, ascomycetous yeast Arxula adeninivorans LS3 is halotolerant. It can grow in a minimal medium containing up to 20% NaCl. The growth parameters are only weakly influenced by 10% NaCl. However, NaCl in a concentration higher than 10% causes a decrease in the specific growth rate, a longer adaptation phase and a lower cell count in the stationary growth phase. Concentrations of glycerol and trehalose, which differed 100-fold in magnitude in a salt free medium, are also influenced differently by salt. NaCl induces accumulation of intracellular glycerol in exponentially growing cells but a reduced concentration of intracellular trehalose in stationary cells. Transcripts of the genes ARFC3, encoding a component of the replication factor C, and GAA, encoding a secretory glucoamylase, can be detected only in cells cultured in media with NaCl concentrations below 10%. Furthermore, NaCl in high concentration reduces the level of secreted proteins including glucoamylase end invertase.
Yang
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Institut für Pflanzengenetik und Kulturpflanzenforschung, Gatersleben, Germany.
Wartmann
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Stoltenburg
R
R
Kunze
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G
D000220:Adaptation, Biological; D001203:Ascomycota / Q000187:drug effects / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000096:biosynthesis; D005656:Fungal Proteins / Q000378:metabolism; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism; D005990:Glycerol / Q000032:analysis; D006026:Glycoside Hydrolases / Q000378:metabolism; D003904:Mitosporic Fungi / Q000187:drug effects / Q000502:physiology; D020648:Peptide Elongation Factor 1 / Q000096:biosynthesis; D012331:RNA, Fungal / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D012965:Sodium Chloride / Q000494:pharmacology*; D014199:Trehalose / Q000032:analysis; D015003:Yeasts / Q000187:drug effects / Q000502:physiology*; D043324:beta-Fructofuranosidase
C118424:ARFC3 protein, Arxula adeninivorans; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D020648:Peptide Elongation Factor 1; D012331:RNA, Fungal; D012333:RNA, Messenger; D012965:Sodium Chloride; D014199:Trehalose; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase; D005087:Glucan 1,4-alpha-Glucosidase; D005990:Glycerol