Inhibitory effect of interleukin-1alpha-induced apoptosis by Polygala tenuifolia in Hep G2 cells.
A human hepatoma cell line, Hep G2 cells are reliable for the study of alcohol-induced hepatotoxicity. In this study, we investigated the effect of an aqueous extract of Polygala tenuifolia WILLDENOW (Polygalaceae) roots (PTAE) on ethanol (EtOH)-induced cytotoxicity in Hep G2 cells. PTAE (0.01-1 microg/ml) dose-dependently inhibited the EtOH-induced interleukin-1alpha (IL-1alpha) secretion. PTAE (0.01-1 microg/ml) also inhibited the EtOH- and IL-1alpha-induced cytotoxicity. Furthermore, we found that PTAE inhibited the IL-1alpha-induced apoptosis of Hep G2 cells. These results suggest that PTAE may prevent the EtOH-induced cytotoxicity through inhibition of the apoptosis of Hep G2 cells.
Koo
H N
HN
Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Chonbuk, South Korea.
Jeong
H J
HJ
Kim
K R
KR
Kim
J C
JC
Kim
K S
KS
Kang
B K
BK
Kim
H M
HM
Kim
J J
JJ
D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D000431:Ethanol / Q000633:toxicity; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism / Q000494:pharmacology*; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D010936:Plant Extracts / Q000494:pharmacology; D010946:Plants, Medicinal; D011994:Recombinant Proteins / Q000494:pharmacology; D019685:Rosales
D007375:Interleukin-1; D010936:Plant Extracts; D011994:Recombinant Proteins; D000431:Ethanol