title: Aspartate aminotransferase activity in crevicular fluid from dental implants.
abstract: BACKGROUND
  Aspartate aminotransferase (AST) is an enzyme normally confined to the cytoplasm of cells, but released to the extracellular environment upon cell death. Its levels are associated with the severity of experimental gingivitis and the loss of periodontal attachment. The aim of the present study was to investigate the presence and activity levels of AST in peri-implant crevicular fluid (PCF) from healthy and diseased endosseous implants in order to assess if AST in PCF can be further studied as a possible objective diagnostic aid in oral implantology.
  
  
  METHODS
  Eighty-one fixtures from 81 systemically healthy subjects were divided into 3 groups, 27 healthy implants (HI), 27 implants with mucositis (MI) and 27 implants affected by peri-implantitis (PI) according to well-defined clinical and radiographic criteria. PCF was collected by the insertion of a #40 standardized endodontic paper point to the base of the crevice or pocket for 30 seconds. AST activity was determined spectrophotometrically at 25 degrees C. The results were expressed as AST Units/ml in PCF.
  
  
  RESULTS
  An AST activity was detected in each sample from HI, MI and PI. The mean AST activity in HI was 0.26 +/- 0.16 U/ml; in MI, 0.38 +/- 0.27 U/ml; in PI, 0.62 +/- 0.29 U/ml. ANOVA showed that the difference among HI, MI, and PI was statistically significant at P <0.01 level. Post-hoc tests demonstrated that a significant difference in AST activity existed between HI/PI (t = 5.14; P<0.01) and MI/PI (t = 3.09; P<0.01). No statistically significant difference was found between HI/MI (t = 1.07; P >0.1) AST activity was significantly (P <0.01) associated with probing depth (r = 0.55), the amount of bone loss (r = 0.60) and bleeding on probing (r = 0.67). When the threshold for a positive AST test was set > or =0.4 U/ml, a sensitivity = 0.81 and a specificity = 0.74 were found in the detection of peri-implantitis; the positive predictive value was 61% and the negative predictive value was 88%.
  
  
  CONCLUSIONS
  Within the limits of this study, our results may suggest that PCF analysis could be further investigated in longitudinal studies as a suitable diagnostic strategy in the evaluation of dental implants.
authors.affiliation: University G. D'Anunzio School of Dentistry, Department of Periodontology, Cheti, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Paolantonio
authors.forename: G
authors.initials: G
authors.lastname: Di Placido
authors.forename: V
authors.initials: V
authors.lastname: Tumini
authors.forename: M
authors.initials: M
authors.lastname: Di Stilio
authors.forename: A
authors.initials: A
authors.lastname: Contento
authors.forename: G
authors.initials: G
authors.lastname: Spoto
mesh_terms: D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D001219:Aspartate Aminotransferases / Q000032:analysis / Q000097:blood / Q000378:metabolism*; D004796:Clinical Enzyme Tests; D003430:Cross-Sectional Studies; D003758:Dental Implantation, Endosseous / Q000009:adverse effects; D015921:Dental Implants / Q000009:adverse effects*; D019232:Dental Restoration Failure; D005260:Female; D005883:Gingival Crevicular Fluid / Q000201:enzymology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000201:enzymology; D016348:Osseointegration; D010518:Periodontitis / Q000175:diagnosis* / Q000209:etiology; D011237:Predictive Value of Tests; D011379:Prognosis; D018709:Statistics, Nonparametric; D013280:Stomatitis / Q000175:diagnosis* / Q000209:etiology
chemical_list: D015921:Dental Implants; D001219:Aspartate Aminotransferases