Introduction of a foreign gene into medakafish using the particle gun method.
We developed a procedure to introduce a foreign gene into fertilized eggs of medakafish (Oryzias latipes) using the particle gun method, which is one of the easiest and most reliable techniques for gene transfer. A plasmid construct with the green fluorescence protein (GFP) gene driven by the madakafish beta-actin gene promoter was successfully introduced into eggs, and the expression of GFP was observed in 20% of the primary transfectant (chimera) fish. In addition, germ line transmission of GFP was observed in 13% of the GFP-positive primary transfectant fish. The new application described here should enable us to investigate gene expression using the fish model on a routine basis without high technical sophistication. J. Exp. Zool. 287:285-293, 2000.
Yamauchi
M
M
Genome Research Group, National Institute of Radiological Sciences, Chiba 263-8555, Japan. m_yam@nirs.go.jp
Kinoshita
M
M
Sasanuma
M
M
Tsuji
S
S
Terada
M
M
Morimyo
M
M
Ishikawa
Y
Y
D000818:Animals; D030801:Animals, Genetically Modified; D019470:Biolistics / Q000379:methods*; D003001:Cloning, Molecular; D005260:Female; D015870:Gene Expression; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins; D007202:Indicators and Reagents / Q000008:administration & dosage / Q000378:metabolism; D008164:Luminescent Proteins / Q000008:administration & dosage / Q000235:genetics* / Q000378:metabolism; D008297:Male; D009990:Oryzias / Q000235:genetics*; D010957:Plasmids / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism; D015053:Zygote / Q000378:metabolism
D007202:Indicators and Reagents; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins