title: Activation of cellular interferon-responsive genes after infection of human cells with herpes simplex virus type 1.
abstract: Previous studies have shown that infection of human fibroblasts with human cytomegalovirus (HCMV) results in activation of cellular interferon-responsive gene expression. We demonstrate here that infection of human fibroblasts with herpes simplex virus type 1 (HSV-1) in the absence of de novo protein synthesis also induces the expression of interferon-responsive genes. Five genes tested (encoding ISG54, IFI56, ISG15, 9-27 and MxA) were activated by infection with HSV-1, although the degree of response varied between the individual genes. HSV-1 was a less efficient inducer than HCMV. The effect was a consequence of binding of the virus particle to the cell surface or of the presence of virion components within the infected cell. Induction was mediated by a pathway other than the mechanism through which interferon-alpha mediates its effects on cellular gene expression.
authors.affiliation: Medical Research Council Virology Unit, Church Street, Glasgow G11 5JR, UK1.
authors.forename: Mary Jane
authors.initials: MJ
authors.lastname: Nicholl
authors.affiliation: Medical Research Council Virology Unit, Church Street, Glasgow G11 5JR, UK1.
authors.forename: Laurence H
authors.initials: LH
authors.lastname: Robinson
authors.affiliation: Medical Research Council Virology Unit, Church Street, Glasgow G11 5JR, UK1.
authors.forename: Chris M
authors.initials: CM
authors.lastname: Preston
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000998:Antiviral Agents / Q000096:biosynthesis; D002352:Carrier Proteins; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis; D005347:Fibroblasts / Q000821:virology; D019204:GTP-Binding Proteins; D015967:Gene Expression Regulation, Viral; D006561:Herpes Simplex / Q000235:genetics*; D018259:Herpesvirus 1, Human; D006801:Humans; D007372:Interferons / Q000502:physiology*; D016350:Leucine Zippers; D064547:Myxovirus Resistance Proteins; D014176:Protein Biosynthesis; D016601:RNA-Binding Proteins; D014407:Tumor Cells, Cultured; D014452:Ubiquitins / Q000031:analogs & derivatives*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D000998:Antiviral Agents; D002352:Carrier Proteins; D016207:Cytokines; C489550:G1p2 protein, mouse; C484290:IFIT1 protein, human; C579071:MX1 protein, human; D064547:Myxovirus Resistance Proteins; D016601:RNA-Binding Proteins; D014452:Ubiquitins; C074034:ISG15 protein, human; D007372:Interferons; D019204:GTP-Binding Proteins