Local and systemic changes in squash gene expression in response to silverleaf whitefly feeding.
Squash genes (SLW1 and SLW3) induced systemically after silverleaf whitefly feeding were identified. Differences in the local and systemic expression of SLW1 and SLW3 after feeding by the closely related silverleaf and sweetpotato whiteflies were observed. Temporal and spatial studies showed that SLW1 and SLW3 were induced when second, third, and fourth nymphal instars were feeding. Although only barely detected after wounding and bacterial infection, SLW1 and SLW3 RNAs were abundant during water-deficit stress. Treatments with wound/defense signal molecules showed that SLW1 RNAs accumulated in response to methyl jasmonate and ethylene, whereas SLW3 was not regulated by known wound/defense signals, suggesting utilization of a novel mechanism for defense signal transduction. SLW1 RNAs accumulated during floral and fruit development, whereas SLW3 RNAs were not detected during vegetative or reproductive development. The potential roles of SLW1, an M20b peptidase-like protein, and SLW3, a beta-glucosidase-like protein, in defense and the leaf-silvering disorder are discussed.
van de Ven
W T
WT
Department of Botany and Plant Sciences, University of California, Riverside, California 92521-0124, USA.
LeVesque
C S
CS
Perring
T M
TM
Walling
L L
LL
D000595:Amino Acid Sequence; D000818:Animals; D016384:Consensus Sequence / Q000235:genetics; D019662:Cucurbitaceae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000469:parasitology*; D004175:Diptera / Q000502:physiology*; D004435:Eating / Q000502:physiology*; D010450:Endopeptidases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics*; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology; D008666:Metalloendopeptidases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009569:Nitric Oxide / Q000378:metabolism / Q000494:pharmacology; D009758:Nymph / Q000502:physiology; D010935:Plant Diseases / Q000235:genetics / Q000469:parasitology; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000469:parasitology; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D018749:RNA, Plant / Q000032:analysis / Q000235:genetics; D016415:Sequence Alignment; D015398:Signal Transduction / Q000187:drug effects; D001617:beta-Glucosidase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D010937:Plant Growth Regulators; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D009569:Nitric Oxide; D006861:Hydrogen Peroxide; C416514:SLW3 protein, Cucurbita pepo; D001617:beta-Glucosidase; D010450:Endopeptidases; C416513:SLW1 protein, Cucurbita pepo; D008666:Metalloendopeptidases