title: Inhibition of wheat leaf nitrate reductase activity by phenolic compounds.
abstract: Phenolic acids inhibited the activities of wheat leaf nitrate reductase depending on phenolic structure and concentration. Possible conformational change(s) in the enzyme induced by hydrogen bonding and/or hydrophobic interactions might be the cause of the enzyme inhibition. NADH:cytochrome C reductase partial activity was unaffected, which indicates that terminal nitrate-reducing domain of NR may be the site of polyphenol binding.
authors.affiliation: Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia. bbassam@ksu.edu.sa
authors.forename: B A
authors.initials: BA
authors.lastname: Albassam
mesh_terms: D002109:Caffeic Acids; D003373:Coumaric Acids; D004791:Enzyme Inhibitors / Q000378:metabolism; D047348:Hydrolyzable Tannins / Q000378:metabolism; D009245:NADH Dehydrogenase / Q000378:metabolism; D050895:Nitrate Reductase (NADH); D009565:Nitrate Reductases / Q000037:antagonists & inhibitors*; D010636:Phenols / Q000378:metabolism; D018515:Plant Leaves / Q000201:enzymology; D011422:Propionates; D014908:Triticum / Q000201:enzymology*; D014641:Vanillic Acid
chemical_list: D002109:Caffeic Acids; D003373:Coumaric Acids; D004791:Enzyme Inhibitors; D047348:Hydrolyzable Tannins; D010636:Phenols; D011422:Propionates; D009245:NADH Dehydrogenase; D009565:Nitrate Reductases; D050895:Nitrate Reductase (NADH); D014641:Vanillic Acid; C495469:p-coumaric acid; C040048:caffeic acid