Determination of the sensitivity of a rapid Escherichia coli O157:H7 assay for testing 375-gram composite samples.
Both 25-g single-size ground beef samples and 375-g composite ground beef samples were tested by a method combining an immunomagnetic separation (IMS) technique with a sandwich enzyme-linked immunosorbent assay (ELISA) system (IMS-ELISA). The results demonstrated that IMS-ELISA could detect the target, Escherichia coli O157:H7, at the level of 10(-1) CFU/g of sample in either the 25- or 375-g sample size.
Tsai
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Richter International, Inc., Columbus, Ohio 43201, USA.
Miller
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Richter
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D000818:Animals; D002417:Cattle; D015169:Colony Count, Microbial; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D019453:Escherichia coli O157 / Q000302:isolation & purification*; D005516:Food Microbiology; D018189:Immunomagnetic Separation / Q000379:methods*; D008461:Meat Products / Q000382:microbiology*; D012680:Sensitivity and Specificity