Evaluation of ceftazidime concentration released in agar from an E test strip.
The aim of this study was to evaluate, using high-performance liquid chromatography, the concentration of ceftazidime in agar released from an E test strip, sampling at the edge of the strip at different points (1, 2, 4, 8, 16, 32, 64, and 128 microg/ml) at 6, 15, and 24 h after its deposition on uninoculated plates. From 6 to 24 h, the ceftazidime concentration in agar increased at the graduations 1, 2, and 4 microg/ml (+140, +82, and +58%, respectively), remained fairly constant at 8 microg/ml (-1.9%), and decreased at 16, 32, 64, and 128 microg/ml (-25, -44, -36, and -58%, respectively). In the 6-24 h range, the ceftazidime concentrations between 16 and 1 microg/ml were +/-1 serial dilution of the values reported on the strip, confirming the accuracy of the E test in agar.
Vaiani
R
R
Department of Laboratory Medicine, Scientific Institute H.S. Raffaele and School of Medicine, Milan, Italy.
Arcelloni
C
C
Comuzzi
B
B
Gesu
G
G
Bonato
C
C
Paroni
R
R
D000362:Agar; D002442:Ceftazidime / Q000032:analysis*; D002511:Cephalosporins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D008826:Microbial Sensitivity Tests / Q000379:methods*; D011934:Reagent Strips
D002511:Cephalosporins; D011934:Reagent Strips; D000362:Agar; D002442:Ceftazidime