Preparation protocols for high-activity photosystem II membrane particles of green algae and higher plants, pH dependence of oxygen evolution and comparison of the S2-state multiline signal by X-band EPR spectroscopy.
Photosystem II (PS II) membrane particles are particularly well suited for various types of spectroscopic investigations on the PS II manganese complex. Here we present: (1) a preparation protocol for PS II membrane particles of higher plants, which yields exceptionally high oxygen-evolution activity due to the use of glycinebetaine as a PS II-stabilizing agent; (2) preparation protocols for highly active PS II membrane particles for the green algae Scenedesmus obliquus and Chlamydomonas reinhardtii; (3) a determination of pH dependence of oxygen evolution for spinach and Scenedesmus; (4) a comparison of the EPR multiline signal observed in the S2-state of green algae and higher plants of PS II membrane particles. A clearly broader type of multiline EPR signal is observed in green algae.
Schiller
H
H
FB Biologie, Philipps-Universität Marburg, Germany.
Dau
H
H
D000818:Animals; D001622:Betaine; D002696:Chlamydomonas / Q000378:metabolism*; D000460:Chlorophyta / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D001467:Hordeum / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007700:Kinetics; D010100:Oxygen / Q000378:metabolism*; D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism*; D045332:Photosystem II Protein Complex; D018724:Spinacia oleracea / Q000378:metabolism*
D007202:Indicators and Reagents; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D001622:Betaine; D010100:Oxygen