title: Regulation of cell cycle and apoptosis by human immunodeficiency virus type 1 Vpr.
abstract: Biological effects of HIV-1 Vpr on CD4(+) cells were studied by an infection system. High-titered HIV-1 stocks pseudotyped with vesicular stomatitis virus G protein were prepared and used to inoculate into CD4(+ )T cells at high multiplicity of infection. Both cell- and virion-associated Vpr were demonstrated to arrest the cell cycle at the G2/M phase, and to induce cell apoptosis. Of note, morphologically apoptotic cells were shown to be arrested at the G2/M stage. No appreciable effect of Vpr on the anti-Fas antibody-mediated apoptosis was observed in this system.
authors.affiliation: Department of Virology, The University of Tokushima School of Medicine, 770-8503, Tokushima, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Fukumori
authors.forename: H
authors.initials: H
authors.lastname: Akari
authors.forename: A
authors.initials: A
authors.lastname: Yoshida
authors.forename: M
authors.initials: M
authors.lastname: Fujita
authors.forename: A H
authors.initials: AH
authors.lastname: Koyama
authors.forename: S
authors.initials: S
authors.lastname: Kagawa
authors.forename: A
authors.initials: A
authors.lastname: Adachi
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects*; D002460:Cell Line; D005434:Flow Cytometry; D016195:G2 Phase; D019204:GTP-Binding Proteins / Q000235:genetics; D016336:Gene Products, vpr / Q000494:pharmacology*; D015497:HIV-1; D006367:HeLa Cells; D006801:Humans; D019169:Jurkat Cells; D008938:Mitosis; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics; D054325:vpr Gene Products, Human Immunodeficiency Virus
chemical_list: D016336:Gene Products, vpr; D054325:vpr Gene Products, Human Immunodeficiency Virus; D019204:GTP-Binding Proteins