Alterations of adenomatous polyposis Coli (APC) gene in oral squamous cell carcinoma.
The present study assessed the roles of Adenomatous Polyposis Coli (APC) tumor suppressor gene during oral carcinogenesis. Reduction of APC transcript levels and APC loss of heterozygosity (LOH) were found in 39% (7/18) and 29% (10/34) cases of oral squamous cell carcinoma (OSCC), respectively. The apparent APC heterozygosity (27%) in non-cancerous matched oral tissue (NCMOT) adjacent to OSCC at an exon 11 locus was significantly lower than normally found in the Taiwanese population (49%). These findings suggest that the allelic status of APC could indicate a cancer risk. No polymorphism of 11307 allele of APC was identified in NCMOT or OSCC. Our data indicated that alterations of APC are frequent molecular changes of OSCC. Advances in understanding of the APC alterations that accompany OSCC development might provide a means for early diagnosis and possibly new therapeutic strategies.
Chang
K W
KW
Faculty of Dentistry and Institute of Oral Biology, National Yang-Ming University, Taipei, Taiwan, ROC. ckcw@ym.edu.tw
Lin
S C
SC
Mangold
K A
KA
Jean
M S
MS
Yuan
T C
TC
Lin
S N
SN
Chang
C S
CS
D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D016009:Chi-Square Distribution; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D017491:Genes, APC; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D018807:Polymorphism, Single-Stranded Conformational; D012334:RNA, Neoplasm / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013624:Taiwan
D004273:DNA, Neoplasm; D012334:RNA, Neoplasm