Influence of enteral diets supplemented with key nutrients on lymphocyte subpopulations in Peyer's patches of endotoxin-boostered mice.
OBJECTIVE
This study was undertaken to compare the effect of different key nutrients on lymphocyte subsets of Peyer's patches (PP) and spleen in endotoxemic mice.


METHODS
Female Balb/c mice were fed over a period of 10 days either with an isocaloric and isonitrogenous control diet (Control), a glutamine enriched diet (Diet I) or a diet containing glutamine, arginine, glycine, and n-3 fatty acids (Diet II). On day 7 the mice were challenged intraperitoneally with 25 microg LPS. The lymphocyte subpopulations (B cells, T cells, CD4+ and CD8+) of PP and spleen were analysed by flow cytometry. Glutathione content of small intestinal mucosa and spleen was determined by HPLC and luminal small intestinal IgA by ELISA.


RESULTS
Both experimental diets increased the number of B and T cells in the PP and that of T cells in the spleen (P<0.01). Glutathione content in PP and spleen was higher under administration of key nutrients (P<0.05). Diet II reduced luminal small intestinal IgA content in comparison to the two other groups.


CONCLUSIONS
The addition of arginine, glycine and n-3 fatty acids to a glutamine supplemented diet does not enhance lymphocyte numbers in PP and spleen, but reduces intestinal IgA content.
Manhart
N
N
Department of Surgical Research, University of Vienna, Austria.
Vierlinger
K
K
Akomeah
R
R
Bergmeister
H
H
Spittler
A
A
Roth
E
E
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D019587:Dietary Supplements; D019446:Endotoxemia; D004750:Enteral Nutrition; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D005973:Glutamine / Q000008:administration & dosage / Q000097:blood / Q000276:immunology; D005978:Glutathione / Q000008:administration & dosage / Q000032:analysis / Q000276:immunology; D007070:Immunoglobulin A / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D010581:Peyer's Patches / Q000737:chemistry / Q000187:drug effects / Q000276:immunology*; D013154:Spleen / Q000737:chemistry / Q000187:drug effects / Q000276:immunology*
D007070:Immunoglobulin A; D005973:Glutamine; D005978:Glutathione