title: Influence of glial growth factor and Schwann cells in a bioresorbable guidance channel on peripheral nerve regeneration.
abstract: Using an established rat peripheral nerve regeneration model, we investigated the role of glial growth factor (GGF) in nerve regeneration in combination with a novel bioresorbable poly(lactic-co-glycolic) acid (PLGA) guide in vivo. Schwann cells, established from a 1-cm segment of excised rat sciatic nerve, were isolated and seeded onto nerve guides with or without GGF (n = 24/group). Living nerve guides were re-established in these animals, and nerve regeneration was assessed over a period of 12 weeks. Histological studies revealed a reduction in the total axon count and the number of myelinated axons in the presence of exogenously added Schwann cells compared to saline controls. In contrast, the addition of GGF alone enhanced the total number of axons and significantly increased the number of blood vessels. Although combining GGF with Schwann cells negated the enhanced numbers of axons and blood vessels seen with GGF alone, this combination resulted in the highest myelination index and the fastest conduction velocities recorded. The PLGA guide material did not trigger any histologically detectable host response and was permissive for nerve regeneration in this animal model. The results from this study demonstrate the potential utility of this guide in vivo and establish a promotional role for GGF in nerve regeneration.
authors.affiliation: Tissue Engineering Laboratory, Robert E. Wise M.D. Research and Education Institute Lahey Clinic, Burlington, Massachusetts, USA.
authors.forename: D J
authors.initials: DJ
authors.lastname: Bryan
authors.forename: A H
authors.initials: AH
authors.lastname: Holway
authors.forename: K K
authors.initials: KK
authors.lastname: Wang
authors.forename: A E
authors.initials: AE
authors.lastname: Silva
authors.forename: D J
authors.initials: DJ
authors.lastname: Trantolo
authors.forename: D
authors.initials: D
authors.lastname: Wise
authors.forename: I C
authors.initials: IC
authors.lastname: Summerhayes
mesh_terms: D000818:Animals; D001369:Axons / Q000187:drug effects / Q000648:ultrastructure; D001672:Biocompatible Materials; D001698:Biomedical Engineering; D004594:Electrophysiology; D005260:Female; D019344:Lactic Acid; D009186:Myelin Sheath / Q000187:drug effects / Q000502:physiology; D018919:Neovascularization, Physiologic / Q000187:drug effects; D009416:Nerve Regeneration / Q000187:drug effects* / Q000502:physiology; D020890:Neuregulin-1 / Q000494:pharmacology*; D010525:Peripheral Nerves / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology
chemical_list: D001672:Biocompatible Materials; D020890:Neuregulin-1; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid