title: Attachment and penetration properties of bovine herpesvirus 1 recombinants expressing pseudorabies virus glycoproteins gC and gB.
abstract: OBJECTIVE
  Bovine herpesvirus 1 (BHV-1) has a relatively narrow host cell range in vitro and in vivo when compared to pseudorabies virus (PrV). The aim of this study was to elucidate whether homologous glycoproteins gC and gB from PrV can function in a heterologous BHV-1 background and whether the expression of these PrV glycoproteins influences the in vitro host cell specificity of BHV-1.
  
  
  METHODS
  We constructed BHV-1 recombinants in which PrV gC and gB were expressed either individually or in combination, and examined their attachment and penetration properties in permissive Madin-Darby bovine kidney (MDBK), semipermissive hamster lung (HmLu-1) and nonpermissive murine embryo fibroblast (A31) cells.
  
  
  RESULTS
  Two BHV-1 recombinants which expressed PrV gC exhibited remarkable competence in virus attachment to cells. The expression of PrV gB improved the virus attachment only a little but penetration, especially into HmLu-1 and A31 cells, was enhanced.
  
  
  CONCLUSIONS
  These results indicate that PrV gC and gB can function in a BHV-1 environment and facilitate virus attachment and penetration by BHV-1.
authors.affiliation: Department of Global Agricultural Sciences, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nakamichi
authors.forename: H
authors.initials: H
authors.lastname: Otsuka
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D006224:Cricetinae; D004279:DNA, Viral / Q000032:analysis; D007242:Herpesvirus 1, Bovine / Q000235:genetics* / Q000502:physiology*; D011558:Herpesvirus 1, Suid / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000378:metabolism; D011995:Recombination, Genetic; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D004279:DNA, Viral; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; C070915:glycoprotein gII, pseudorabies virus; C042966:pseudorabies virus glycoproteins