Activation of cellular interferon-responsive genes after infection of human cells with herpes simplex virus type 1.
Previous studies have shown that infection of human fibroblasts with human cytomegalovirus (HCMV) results in activation of cellular interferon-responsive gene expression. We demonstrate here that infection of human fibroblasts with herpes simplex virus type 1 (HSV-1) in the absence of de novo protein synthesis also induces the expression of interferon-responsive genes. Five genes tested (encoding ISG54, IFI56, ISG15, 9-27 and MxA) were activated by infection with HSV-1, although the degree of response varied between the individual genes. HSV-1 was a less efficient inducer than HCMV. The effect was a consequence of binding of the virus particle to the cell surface or of the presence of virion components within the infected cell. Induction was mediated by a pathway other than the mechanism through which interferon-alpha mediates its effects on cellular gene expression.
Nicholl
Mary Jane
MJ
Medical Research Council Virology Unit, Church Street, Glasgow G11 5JR, UK1.
Robinson
Laurence H
LH
Medical Research Council Virology Unit, Church Street, Glasgow G11 5JR, UK1.
Preston
Chris M
CM
Medical Research Council Virology Unit, Church Street, Glasgow G11 5JR, UK1.
D048868:Adaptor Proteins, Signal Transducing; D000998:Antiviral Agents / Q000096:biosynthesis; D002352:Carrier Proteins; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis; D005347:Fibroblasts / Q000821:virology; D019204:GTP-Binding Proteins; D015967:Gene Expression Regulation, Viral; D006561:Herpes Simplex / Q000235:genetics*; D018259:Herpesvirus 1, Human; D006801:Humans; D007372:Interferons / Q000502:physiology*; D016350:Leucine Zippers; D064547:Myxovirus Resistance Proteins; D014176:Protein Biosynthesis; D016601:RNA-Binding Proteins; D014407:Tumor Cells, Cultured; D014452:Ubiquitins / Q000031:analogs & derivatives*
D048868:Adaptor Proteins, Signal Transducing; D000998:Antiviral Agents; D002352:Carrier Proteins; D016207:Cytokines; C489550:G1p2 protein, mouse; C484290:IFIT1 protein, human; C579071:MX1 protein, human; D064547:Myxovirus Resistance Proteins; D016601:RNA-Binding Proteins; D014452:Ubiquitins; C074034:ISG15 protein, human; D007372:Interferons; D019204:GTP-Binding Proteins