title: Development of a 1.4-Mb BAC/PAC contig and physical map within the critical region for complete X-linked congenital stationary night blindness in Xp11.4.
abstract: A physical map internal to the markers DXS1368 and DXS228 was developed for the p11.4 region of the human X chromosome. Twenty-four BACs and 10 PACs with an average insert size of 149 kb were aligned to form a contig across an estimated 1.4 Mb of DNA. This contig, which has on average fourfold clone coverage, was assembled by STS and EST content analysis using 46 markers, including 8 ESTs, two retinally expressed genes, and 22 new STSs developed from BAC- and PAC-derived DNA sequence. The average intermarker distance was 30 kb. This physical map provides resources for high-resolution mapping as well as suitable clones for large-scale sequencing efforts in Xp11.4, a region known to contain the gene for complete X-linked congenital stationary night blindness.
authors.affiliation: Department of Medical Genetics, University of Calgary, Alberta, T2N 4N1, Canada.
authors.forename: R L
authors.initials: RL
authors.lastname: Sparkes
authors.forename: C G
authors.initials: CG
authors.lastname: Summer
authors.forename: K M
authors.initials: KM
authors.lastname: Boycott
authors.forename: R J
authors.initials: RJ
authors.lastname: Zahorchak
authors.forename: N T
authors.initials: NT
authors.lastname: Bech-Hansen
mesh_terms: D001435:Bacteriophages / Q000235:genetics; D002876:Chromosomes, Bacterial / Q000235:genetics; D004247:DNA / Q000235:genetics*; D008040:Genetic Linkage; D006801:Humans; D018895:Microsatellite Repeats; D009755:Night Blindness / Q000151:congenital / Q000235:genetics*; D020161:Physical Chromosome Mapping; D016324:Sequence Tagged Sites; D014960:X Chromosome / Q000235:genetics*
chemical_list: D004247:DNA