High-speed counter-current chromatography of apple procyanidins.
Apple procyanidins were separated by high-speed counter-current chromatography using a type-J multilayer coil planet centrifuge. Several two-phase solvent systems with a wide range of hydrophobicities from a non-polar hexane system to polar n-butanol systems were evaluated their performance in terms of the partition coefficient and the retention of the phase. The best separation of procyanidins B and C was achieved with a two-phase solvent system composed of n-butanol-methyl tert.-butyl ether-acetonitrile-0.1% trifluoroacetic acid (2:4:3:8) using the lower phase as a mobile at a flow-rate of 1.0 ml/min.
Shibusawa
Y
Y
Department of Analytical Chemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Japan.
Yanagida
A
A
Ito
A
A
Ichihashi
K
K
Shindo
H
H
Ito
Y
Y
D044946:Biflavonoids; D002392:Catechin / Q000302:isolation & purification*; D002845:Chromatography / Q000379:methods*; D005638:Fruit / Q000737:chemistry*; D044945:Proanthocyanidins
D044946:Biflavonoids; D044945:Proanthocyanidins; C017674:procyanidin; D002392:Catechin