Crystallization and preliminary X-ray crystallographic analysis of water channel AQP1.
Aquaporin-1 (AQP1), a water channel from bovine red blood cells has been deglycosylated, purified to homogeneity and crystallized in a form suitable for X-ray crystallographic study. Crystals are grown using polyethylene glycol as precipitant and belong to the tetragonal space group I422, with unit-cell parameters a = b = 93.4, c = 180.4 A. The crystals diffract beyond 2.2 A resolution.
Sui
H
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Life Sciences Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, California 94720, USA.
Walian
P J
PJ
Tang
G
G
Oh
A
A
Jap
B K
BK
D000818:Animals; D051398:Aquaporin 1; D020346:Aquaporins / Q000097:blood / Q000737:chemistry*; D002417:Cattle; D011232:Chemical Precipitation; D003460:Crystallization; D018360:Crystallography, X-Ray; D004910:Erythrocyte Membrane / Q000737:chemistry; D006031:Glycosylation
D020346:Aquaporins; D051398:Aquaporin 1