title: Evidence of subtype B-like sequences in the V3 loop region of human immunodeficiency virus type 1 in Kilimanjaro, Tanzania.
abstract: The aim of this study was to determine the nucleotide sequence of the third variable (V3) domain of HIV-1 gp120 from strains circulating in the Kilimanjaro region of Tanzania. DNA from this region was amplified from patient peripheral blood lymphocytes using polymerase chain reaction and then subjected to automated DNA sequencing. Subtype A, B, C, and D-like sequences were identified. Subtype B has not previously been described in Tanzania.
authors.affiliation: Centre for International Health, University of Bergen, Norway.
authors.forename: I E
authors.initials: IE
authors.lastname: Kiwelu
authors.forename: H L
authors.initials: HL
authors.lastname: Nakkestad
authors.forename: J
authors.initials: J
authors.lastname: Shao
authors.forename: M A
authors.initials: MA
authors.lastname: Sommerfelt
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D016000:Cluster Analysis; D004279:DNA, Viral / Q000235:genetics; D015699:HIV Envelope Protein gp120 / Q000737:chemistry* / Q000235:genetics*; D015658:HIV Infections / Q000097:blood / Q000276:immunology / Q000821:virology*; D015497:HIV-1 / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008214:Lymphocytes / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D013636:Tanzania
chemical_list: D004279:DNA, Viral; D015699:HIV Envelope Protein gp120