title: Head-to-tail polymerization of coagulin, a clottable protein of the horseshoe crab.
abstract: A clottable protein coagulogen of the horseshoe crab Tachypleus tridentatus is proteolytically converted into an insoluble coagulin gel through non-covalent self-polymerization. Here we identified binding sites for the polymerization. A tryptic fragment, derived from the coagulin polymer chemically cross-linked by a bifunctional cross-linker, was isolated. Amino acid sequence analysis indicated that the fragment consists of two peptides cross-linked between Lys(85) and Lys(156). The two lysine residues are oppositely located at the head and tail regions of the elongated molecule separated by a much greater distance than the length of the cross-linker, which suggests that the cross-linking occurs intermolecularly. Based on the x-ray structural analysis, exposure of a hydrophobic cove on the head in response to the release of peptide C has been postulated (Bergner, A., Oganessyan, V., Muta, T., Iwanaga, S., Typke, D., Huber, R., and Bode, W. (1996) EMBO J. 15, 6789-6797). An octapeptide containing Tyr(136), which occupies the tail end of coagulin, was found to inhibit the polymerization. Replacement of Tyr(136) of the peptide with Ala resulted in loss of the inhibitory activity. These results indicated that the polymerization of coagulin proceeds through the interaction between the newly exposed hydrophobic cove on the head and the wedge-shaped hydrophobic tail.
authors.affiliation: Department of Biology and Department of Chemistry, Graduate School of Sciences, Kyushu University, Fukuoka 812-8581, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kawasaki
authors.forename: T
authors.initials: T
authors.lastname: Nose
authors.forename: T
authors.initials: T
authors.lastname: Muta
authors.forename: S
authors.initials: S
authors.lastname: Iwanaga
authors.forename: Y
authors.initials: Y
authors.lastname: Shimohigashi
authors.forename: S
authors.initials: S
authors.lastname: Kawabata
mesh_terms: D000409:Alanine / Q000737:chemistry; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002851:Chromatography, High Pressure Liquid; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006737:Horseshoe Crabs; D007700:Kinetics; D008239:Lysine / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010452:Peptide Biosynthesis; D010455:Peptides / Q000737:chemistry; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D020349:Surface Plasmon Resonance; D013925:Thromboplastin / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism*; D013997:Time Factors; D014443:Tyrosine / Q000737:chemistry; D014965:X-Rays
chemical_list: D003432:Cross-Linking Reagents; D010455:Peptides; D014443:Tyrosine; D013925:Thromboplastin; D008239:Lysine; D000409:Alanine