title: Non-conventional yeasts as hosts for heterologous protein production.
abstract: Yeasts are an attractive group of lower eukaryotic microorganisms, some of which are used in several industrial processes that include brewing, baking and the production of a variety of biochemical compounds. More recently, yeasts have been developed as host organisms for the production of foreign (heterologous) proteins. Saccharomyces cerevisiae has usually been the yeast of choice, but an increasing number of alternative non-Saccharomyces yeasts has now become accessible for modern molecular genetics techniques. Some of them exhibit certain favourable traits such as high-level secretion or very strong and tightly regulated promoters, offering significant advantages over traditional bakers' yeast. In the present work, the current status of Kluyveromyces lactis, Yarrowia lipolytica, Hansenula polymorpha and Pichia pastoris (the best-known alternative yeast systems) is reviewed. The advantages and limitations of these systems are discussed in relation to S. cerevisiae.
authors.affiliation: Departamento de Microbiología y Genética/Instituto de Microbiología Bioquímica/CSIC, Universidad de Salamanca, Spain. ado@gugu.usal.es
authors.forename: A
authors.initials: A
authors.lastname: Domínguez
authors.forename: E
authors.initials: E
authors.lastname: Fermiñán
authors.forename: M
authors.initials: M
authors.lastname: Sánchez
authors.forename: F J
authors.initials: FJ
authors.lastname: González
authors.forename: F M
authors.initials: FM
authors.lastname: Pérez-Campo
authors.forename: S
authors.initials: S
authors.lastname: García
authors.forename: A B
authors.initials: AB
authors.lastname: Herrero
authors.forename: A
authors.initials: A
authors.lastname: San Vicente
authors.forename: J
authors.initials: J
authors.lastname: Cabello
authors.forename: M
authors.initials: M
authors.lastname: Prado
authors.forename: F J
authors.initials: FJ
authors.lastname: Iglesias
authors.forename: A
authors.initials: A
authors.lastname: Choupina
authors.forename: F J
authors.initials: FJ
authors.lastname: Burguillo
authors.forename: L
authors.initials: L
authors.lastname: Fernández-Lago
authors.forename: M C
authors.initials: MC
authors.lastname: López
mesh_terms: D015966:Gene Expression Regulation, Fungal; D012331:RNA, Fungal; D011994:Recombinant Proteins / Q000096:biosynthesis*; D015003:Yeasts / Q000235:genetics / Q000378:metabolism* / Q000502:physiology
chemical_list: D012331:RNA, Fungal; D011994:Recombinant Proteins