Contribution to the biological assessment of orthodontic acrylic materials. Measurement of their residual monomer output and cytotoxicity.
The acrylic materials used in orthodontics for the fabrication of removable appliances are subjected in the oral cavity to processes of change which influence their physical, mechanical and biological properties. It is therefore essential that every newly developed material must be judged in terms of its clinical value. In the present study, 2 orthodontic cold-cure acrylics, Orthocryl and Forestacryl, and 4 orthodontic photocure acrylics, Triad, Wil-O-Dont, Odontolux and Lux-A-Tech, were investigated and compared with 2 prosthetic acrylic materials: the cold-cure acrylic Palapress and the hot-cure acrylic Paladon. The quantity of residual monomers from methyl methacrylate (MAA) or urethane dimethacrylate (UDMA) eluted from the sample in a given time after the processing was estimated by high pressure liquid chromatography (HPLC). The cytotoxic properties of the materials were examined by Mosmann's proliferation-inhibition test with an established culture of fibroblasts (= MTT test). The hot-cure acrylic Paladon produced by far the smallest amount of eluted residual monomer and the least growth inhibition in the MTT test. The prosthetic cold-cure acrylic Palapress achieved significantly better results than the orthodontic cold-cure materials Orthocryl and Forestacryl. The photocure acrylics released less UDMA than did the cold-cure acrylics MMA. In the cell culture test, all the orthodontic materials examined were assessed as "slightly cytotoxic"; the prosthetic acrylics were graded under ISO-standard 10993-5 as "noncytotoxic". After soaking the plastic material in water for 3 days its cytotoxic properties, as exemplified by the cold-cure acrylic Forestacryl and the photocure acrylic Triad, were reduced, and during the following investigation no more inhibition of growth was observed. It was possible to confirm with the tests used that, for Triad, it is necessary to carefully remove the oxygen-inhibition layer of the photocure acrylic in order to improve the biological properties. The influence of the plastic material on fibroblast cultures was assessed, among other methods, by the quantity of residual monomers liberated. These were significantly reduced after soaking the manufactured substance in water for 3 days. Careful laboratory treatment of the photocure acrylics is necessary in order to improve their biological properties.
Rose
E C
EC
Department of Orthodontics, University of Freiburg, Germany. rose@zmk2.ukl.uni-freiburg.de
Bumann
J
J
Jonas
I E
IE
Kappert
H F
HF
D000180:Acrylic Resins / Q000032:analysis / Q000633:toxicity*; D000818:Animals; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D003764:Dental Materials / Q000032:analysis / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D007739:L Cells; D008422:Materials Testing / Q000379:methods / Q000706:statistics & numerical data; D051379:Mice; D009968:Orthodontic Appliances, Removable
D000180:Acrylic Resins; D003764:Dental Materials