Comparison of the inactivation of canine and bovine parvovirus by freeze-drying and dry-heat treatment in two high purity factor VIII concentrates.
The inactivation of bovine parvovirus (BPV) and canine parvovirus (CPV) by freeze-drying and terminal dry-heat treatment at 80 degrees C for 72 h has been investigated in two high purity factor VIII concentrates. In one product, CPV was slightly more resistant to freeze-drying compared to BPV, i.e. 0.7 vs. 1.4 log. However, BPV was substantially more resistant to heat-treatment compared to CPV, i.e. 1.3 vs. > 3.1 log inactivation after 72 h at 80 degrees C. In a second product, CPV was also slightly more resistant to freeze-drying than BPV, i.e. 0.2 vs. 1.3 log inactivation. However, heat-treatment gave essentially similar inactivation for both viruses, i.e. 2.8-3.4 log after 72 h at 80 degrees C. In conclusion, the resistance of these parvovirus models is dependent both on the type of virus and on the specific product involved.
Roberts
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PL
Bio Products Laboratory, Elstree, England. peter.roberts@bpl.co.uk
Hart
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H
D000818:Animals; D002417:Cattle; D004285:Dogs; D004340:Drug Contamination; D005169:Factor VIII / Q000302:isolation & purification / Q000592:standards*; D005612:Freeze Drying; D006358:Hot Temperature; D006801:Humans; D017992:Parvovirus / Q000302:isolation & purification*; D017993:Parvovirus, Canine / Q000302:isolation & purification*
D005169:Factor VIII