[Construction of reference panel of immune serum against hepatitis C virus with standard level of IgG antibodies].
A panel of anti-HCV sera (lot 03HC) was prepared from human sera obtained at blood transfusion centers and infectious hospitals. Donor sera and high-titer sera from patients infected with HCV were used. For positive samples, specific sera reactive with the core and/or NS proteins of HCV 1b and 2 were selected. Positive sera were standardized by the concentrations of IgG with a pool of negative sera containing no HBsAg and antibodies to HIV, HCV, and syphilis. The sera for the panel were selected and titered in screening and specific tests. The anti-HCV panel includes negative and positive sera with low and high titers. The panel sera are stabilized and can be stored for a short time at room temperature. The anti-HCV panel of sera, lot 02HC, was certified at L. A. Tarasevich Institute for Standardization and Control as anti-HCV reference panel intended for sensitivity, specificity, and stability control of diagnostic systems for detection of antibodies to HCV in Russia.
Kanev
A N
AN
Shalunova
N V
NV
Netesov
S V
SV
Iastrebova
O N
ON
Musina
E E
EE
Bocharova
N G
NG
Mishin
V P
VP
Kiselev
N N
NN
Shystov
A V
AV
Smerdova
M A
MA
Bektimirov
T A
TA
D000914:Antibodies, Viral / Q000097:blood*; D000918:Antibody Specificity; D016174:Hepacivirus / Q000276:immunology*; D006801:Humans; D007106:Immune Sera; D007118:Immunoassay; D007074:Immunoglobulin G / Q000097:blood*; D012015:Reference Standards
D000914:Antibodies, Viral; D007106:Immune Sera; D007074:Immunoglobulin G