title: Genomic sequence analysis of the mouse Naip gene array.
abstract: A mouse locus called Lgn1 determines differences in macrophage permissiveness for the intracellular replication of Legionella pneumophila. The only regional candidate genes for this phenotype difference lie within a cluster of closely linked paralogs of the Neuronal Apoptosis Inhibitory Protein (Naip) gene. Previous genetic and physical mapping of the Lgn1 phenotype narrowed it to an interval containing only Naip2 and Naip5, suggesting that there is not complete functional overlap among the mouse Naip loci. In order to gather more information about polymorphisms among the Naip genes of the 129 mouse haplotype, we have determined the genomic sequence of a substantial portion of the 129 Naip gene array. We have constructed an evolutionary model for the expansion of the Naip gene array from a single progenitor Naip gene. This model predicts the presence of two distinct families of Naip paralogs: Naip1/2/3 and Naip4/5/6/7. Unlike the divergences among all the other Naip paralogs, the splits among Naip4, Naip5, Naip6, and Naip7 occurred relatively recently. The high degree of sequence conservation within the Naip4/5/6/7 family increases the likelihood of functional overlap among these genes.
authors.affiliation: Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: M G
authors.initials: MG
authors.lastname: Endrizzi
authors.forename: V
authors.initials: V
authors.lastname: Hadinoto
authors.forename: J D
authors.initials: JD
authors.lastname: Growney
authors.forename: W
authors.initials: W
authors.lastname: Miller
authors.forename: W F
authors.initials: WF
authors.lastname: Dietrich
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D005819:Genetic Markers; D016678:Genome; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D009419:Nerve Tissue Proteins / Q000235:genetics*; D051616:Neuronal Apoptosis-Inhibitory Protein; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D005819:Genetic Markers; C496804:Naip 3 pseudogene, mouse; C496801:Naip1 protein, mouse; C496802:Naip2 protein, mouse; C496805:Naip4 protein, mouse; C496806:Naip5 protein, mouse; C496807:Naip6 protein, mouse; C496808:Naip7 protein, mouse; D009419:Nerve Tissue Proteins; D051616:Neuronal Apoptosis-Inhibitory Protein