Ligand-presenting assembly: a method for C- and N-terminal antigen presentation.
Achiral dicarboxylic acids were coupled with 2 eq. of the free alpha-amino groups of two fully side-chain protected peptide chains while these were still attached to a synthesis resin. Cleavage from the resin with simultaneous side-chain deprotection afforded two assembled peptide chains with free C-terminals. Suitable functionalization of the achiral dicarboxylic acid alternatively permitted continued peptide synthesis in a C to N orientation leading to a final peptide assembly which, after cleavage from the resin, may have multiple N to C and C to N presentation of one or more epitopes.
Holm
A
A
Research Center for Medical Biotechnology, Chemistry Department, The Royal Veterinary and Agricultural University, Copenhagen, Denmark. arho@kvl.dk
Jørgensen
R M
RM
Ostergaard
S
S
Theisen
M
M
D000595:Amino Acid Sequence; D000818:Animals; D000942:Antigens, Bacterial / Q000096:biosynthesis / Q000737:chemistry / Q000276:immunology*; D001898:Borrelia / Q000276:immunology; D002244:Carbon / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D018985:Epitopes, B-Lymphocyte / Q000737:chemistry*; D008024:Ligands; D051379:Mice; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000737:chemistry
D000942:Antigens, Bacterial; D018985:Epitopes, B-Lymphocyte; D008024:Ligands; D002244:Carbon; D009584:Nitrogen