Protein kinase C alpha-dependent phosphorylation of Golgi proteins.
Golgi-enriched membranes were phosphorylated in order to understand the mechanism for protein kinase C (PKC) regulation of exocytic vesicle formation at the trans-Golgi network. Two of the main PKC substrates were identified as MARCKS and Mac-MARCKS by two-dimensional electrophoresis (2-DE) and mass spectrometric sequencing. Annexin IV and profilin I, two other Golgi-associated proteins--although known as in vitro PKC substrates--were not phosphorylated in the Golgi-bound state.
Radau
B
B
Cell Biology, Max-Delbrück-Center for Molecular Medicine, Berlin, Germany.
Otto
A
A
Müller
E C
EC
Westermann
P
P
D017314:Annexin A4 / Q000378:metabolism; D015153:Blotting, Western; D002148:Calmodulin-Binding Proteins; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology; D003285:Contractile Proteins; D015180:Electrophoresis, Gel, Two-Dimensional; D006056:Golgi Apparatus / Q000378:metabolism*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D007527:Isoenzymes / Q000502:physiology*; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology; D008565:Membrane Proteins / Q000378:metabolism*; D008840:Microfilament Proteins / Q000378:metabolism; D000076250:Myristoylated Alanine-Rich C Kinase Substrate; D009363:Neoplasm Proteins / Q000378:metabolism; D010766:Phosphorylation; D051304:Profilins; D011493:Protein Kinase C / Q000502:physiology*; D051571:Protein Kinase C-alpha; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000378:metabolism*; D050683:R-SNARE Proteins; D021241:Spectrometry, Mass, Electrospray Ionization; D013379:Substrate Specificity; D014407:Tumor Cells, Cultured / Q000378:metabolism
D017314:Annexin A4; D002148:Calmodulin-Binding Proteins; D003285:Contractile Proteins; D047908:Intracellular Signaling Peptides and Proteins; D007527:Isoenzymes; C000619710:MARCKS protein, human; C070177:MARCKSL1 protein, human; D008565:Membrane Proteins; D008840:Microfilament Proteins; D009363:Neoplasm Proteins; C495738:PFN1 protein, human; D051304:Profilins; D011506:Proteins; D050683:R-SNARE Proteins; C112938:VAMP8 protein, human; D000076250:Myristoylated Alanine-Rich C Kinase Substrate; C496628:PRKCA protein, human; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha