title: Multiple, closely spaced alternative 5' exons in the DmCKIIbeta gene of Drosophila melanogaster.
abstract: Drosophila melanogaster casein kinase II (CKII) is composed of catalytic alpha and regulatory beta subunits. Using the two-hybrid system, we have isolated a number of cDNAs that are related to a previously published cDNA encoding the beta subunit, but exhibit divergent 5' sequences. To determine the source of this sequence variation, we have isolated the gene encoding the beta subunit of CKII. The beta gene contains five exons encompassing the complete open reading frame, as well as five alternative exons in the 5' untranslated region (UTR). Only one 5' UTR exon is contained in each cDNA, implying five distinct classes of transcript. In addition, the beta gene contains at least two poly(A) addition signals which generate additional complexity at the 3' end. The complex pattern of transcription may serve a role in the spatial and/or temporal expression of the beta subunit since, with one exception, all transcripts encode the full-length beta polypeptide. Phylogenetic comparison of the beta genes of Drosophila, C. elegans, and mammals reveals three invariant introns as well as evidence of recent intron gain/loss.
authors.affiliation: Department of Biology, West Virginia University, Morgantown, West Virginia 26506-6057, USA. abidwai@wvu.edu
authors.forename: A P
authors.initials: AP
authors.lastname: Bidwai
authors.forename: A
authors.initials: A
authors.lastname: Saxena
authors.forename: W
authors.initials: W
authors.lastname: Zhao
authors.forename: R O
authors.initials: RO
authors.lastname: McCann
authors.forename: C V
authors.initials: CV
authors.lastname: Glover
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D047390:Casein Kinase II; D002874:Chromosome Mapping; D002875:Chromosomes / Q000648:ultrastructure; D018076:DNA, Complementary / Q000032:analysis / Q000302:isolation & purification; D004268:DNA-Binding Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics*; D005091:Exons / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D017385:Sequence Homology; D020798:Two-Hybrid System Techniques
chemical_list: D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases