Myeloid dendritic cells induce Th2 responses to inhaled antigen, leading to eosinophilic airway inflammation.
The aim of this study was to investigate whether dendritic cells (DCs) can induce sensitization to aeroallergen in a mouse model of allergic asthma. Ovalbumin-pulsed (OVA-pulsed) or unpulsed myeloid DCs that were injected into the airways of naive mice migrated into the mediastinal lymph nodes. When challenged 2 weeks later with an aerosol of OVA, activated CD4 and CD8 lymphocytes, eosinophils, and neutrophils were recruited to the lungs of actively immunized mice. These CD4(+) lymphocytes produced predominantly IL-4 and IL-5 but also IFN-gamma, whereas CD8(+) lymphocytes produced predominantly IFN-gamma. Histological analysis revealed perivascular and peribronchial eosinophilic infiltrates and goblet cell hyperplasia. Studies in IL-4(-/-) and CD28(-/-) mice revealed that production of IL-4 by host cells and provision of costimulation to T cells by DCs were critical for inducing the response. Lung CD4(+) T cells strongly expressed the Th2 marker T1/ST2, and signaling through this molecule via a ligand expressed on DCs was essential for the establishment of airway eosinophilia. These data demonstrate that DCs in the airways induce sensitization to inhaled antigen and that molecules expressed on the surface of these cells are critical for the development of Th2-dependent airway eosinophilia.
Lambrecht
B N
BN
Department of Pulmonary Medicine, Erasmus University Rotterdam, Rotterdam, The Netherlands. lambrecht@lond.azr.nl
De Veerman
M
M
Coyle
A J
AJ
Gutierrez-Ramos
J C
JC
Thielemans
K
K
Pauwels
R A
RA
D000280:Administration, Inhalation; D000485:Allergens / Q000008:administration & dosage; D000818:Animals; D000941:Antigens / Q000008:administration & dosage*; D001249:Asthma / Q000209:etiology* / Q000276:immunology / Q000473:pathology; D018106:CD28 Antigens / Q000235:genetics / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology*; D004195:Disease Models, Animal; D015847:Interleukin-4 / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008817:Mice, Mutant Strains; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology; D011657:Pulmonary Eosinophilia / Q000209:etiology* / Q000276:immunology / Q000473:pathology; D015398:Signal Transduction; D018418:Th2 Cells / Q000276:immunology*
D000485:Allergens; D000941:Antigens; D018106:CD28 Antigens; D016207:Cytokines; D015847:Interleukin-4; D010047:Ovalbumin