title: Histological rearrangement in the facial nerve and central nuclei following immediate and delayed hypoglossal-facial nerve anastomosis.
abstract: The timing of hypoglossal-facial nerve anastomosis is controversial. The present study was performed to clarify the influence of the timing of hypoglossal-facial nerve anastomosis on histological changes in the facial nerve and central nuclei using guinea pigs. The facial nerve was transected first at the labyrinthine portion, and then transected again at the stylomastoid foramen. Hypoglossal-facial nerve anastomosis was carried out immediately or 3 months later. Nerve regeneration and survival of the neurons in the facial and hypoglossal nuclei were evaluated by toluidine blue staining and horseradish peroxidase (HRP). Immediate anastomosis resulted in better nerve regeneration of the facial nerve, but the numbers of surviving neurons in the facial and hypoglossal nuclei were almost the same in both the immediate and delayed anastomosis groups.
authors.affiliation: Department of Otolaryngology, National Taiwan University Hospital, Taipei, Republic of China. sos7071@ms7.hinet.net
authors.forename: Y S
authors.initials: YS
authors.lastname: Chen
authors.forename: C J
authors.initials: CJ
authors.lastname: Hsu
authors.forename: T C
authors.initials: TC
authors.lastname: Liu
authors.forename: N
authors.initials: N
authors.lastname: Yanagihara
authors.forename: S
authors.initials: S
authors.lastname: Murakami
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D005154:Facial Nerve / Q000473:pathology / Q000502:physiology / Q000601:surgery*; D006168:Guinea Pigs; D007002:Hypoglossal Nerve / Q000473:pathology / Q000502:physiology / Q000601:surgery*; D009416:Nerve Regeneration; D013997:Time Factors