Characterization of tachyzoite and bradyzoite antigens of Toxoplasma gondii recognized by immunoglobulins G, M and A in an experimental model of reactivated toxoplasmosis.
Toxoplasma gondii tachyzoite and bradyzoite antigens that are recognized during reactivated toxoplasmosis were studied in comparison with those recognized during acute and chronic infections, in murine models of toxoplasmosis. Both tachyzoite and bradyzoite antigens of the RH and KSU T. gondii strains, respectively, were resolved by sodium- dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) then probed by immunoblot (IB) (Western blot) with mice antisera elicited by different states of toxoplasmosis to allow parallel analysis of antigen recognition by immunoglobulin G, M, and A antibodies. T. gondii tachyzoite and bradyzoite antigens were well studied by IB recording some specific and commonly shared antigens. A complex array of T. gondii tachyzoite and bradyzoite antigens was revealed in the three states of toxoplasmosis. During reactivated toxoplasmosis the commonly shared tachyzoite and bradyzoite antigens were the dominantly recorded antigens. Also, antigens of tachyzoite at 48 KDa and bradyzoite at 40 KDa were recognized in reactivated toxoplasmosis by IgM and IgG, respectively, and not in other states by the same immunoglobulin. The virulent RH and avirulent KSU strains of T. gondii showed some similarities.
el-Shennawy
S F
SF
Department of Parasitology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
el-Gozamy
B M
BM
Mahmoud
M S
MS
Thabet
H S
HS
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007070:Immunoglobulin A / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis*; D051379:Mice; D008970:Molecular Weight; D014122:Toxoplasma / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000276:immunology*
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M