Rapid degradation of ferulic acid via 4-vinylguaiacol and vanillin by a newly isolated strain of bacillus coagulans.
A new strain Bacillus coagulans BK07 was isolated from decomposed wood-bark, based on its ability to grow on ferulic acid as a sole carbon source. This strain rapidly decarboxylated ferulic acid to 4-vinylguaiacol, which was immediately converted to vanillin and then oxidized to vanillic acid. Vanillic acid was further demethylated to protocatechuic acid. Above 95% substrate degradation was obtained within 7 h of growth on ferulic acid medium, which is the shortest period of time reported to date. The major degradation products, was isolated and identified by thin-layer chromatography, high performance liquid chromatography and 1H-nuclear magnetic resonance spectroscopy were 4-vinylguaiacol, vanillin, vanillic acid and protocatechuic acid.
Karmakar
B
B
Department of Biotechnology, Panjab University, Chandigarh, India.
Vohra
R M
RM
Nandanwar
H
H
Sharma
P
P
Gupta
K G
KG
Sobti
R C
RC
D001407:Bacillus / Q000145:classification / Q000378:metabolism*; D001547:Benzaldehydes / Q000378:metabolism*; D001673:Biodegradation, Environmental; D001709:Biotechnology / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000737:chemistry / Q000493:pharmacokinetics*; D006139:Guaiacol / Q000031:analogs & derivatives* / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D014197:Trees / Q000382:microbiology; D014934:Wood
D001547:Benzaldehydes; D003373:Coumaric Acids; D006139:Guaiacol; C014245:4-vinylguaiacol; C004999:ferulic acid; C100058:vanillin