Purification and characterization of extracellular superoxide dismutase in mouse lung.
Extracellular superoxide dismutase (EC-SOD) is the major isozyme of SOD in arteries, but is also abundant in lungs. In particular, mouse lungs contain large amounts of EC-SOD compared to lungs in other mammals. This suggests that EC-SOD may have an amplified function in the mouse lung. This study describes the purification and characterization of mouse EC-SOD as well as its localization in mouse lung. Mouse EC-SOD exists primarily as a homotetramer composed of a pair of dimers linked through disulfide bonds present in the heparin-binding domains of each subunit. In addition, mouse EC-SOD can exist in active multimeric forms. We developed and utilized a polyclonal antibody to mouse EC-SOD to immunolocalize EC-SOD in mouse lung. EC-SOD labeling is strongest in the matrix of vessels, airways, and alveolar septa. This localization suggests that EC-SOD may have important functions in pulmonary biology, perhaps in the modulation of nitric oxide-dependent responses.
Fattman
C L
CL
Department of Pathology, University of Pittsburgh Medical Center, Scaife Hall, Seventh Floor, Pittsburgh, Pennsylvania 15261, USA.
Enghild
J J
JJ
Crapo
J D
JD
Schaefer
L M
LM
Valnickova
Z
Z
Oury
T D
TD
D000595:Amino Acid Sequence; D000818:Animals; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D007150:Immunohistochemistry; D008168:Lung / Q000201:enzymology*; D051379:Mice; D008969:Molecular Sequence Data; D013482:Superoxide Dismutase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism
D013482:Superoxide Dismutase