Comparison of rapid centrifugation assay with conventional tissue culture method for isolation of dengue 2 virus in C6/36-HT cells.
A rapid centrifugation assay was compared with conventional tube cell culture for dengue virus isolation in both sera and autopsy samples from dengue and dengue hemorrhagic fever/dengue shock syndrome fatal cases. The rapid centrifugation assay allowed isolation of virus from 16.6% more samples than the conventional method, and it shortened the time for dengue virus detection. Finally, it allowed the isolation of dengue 2 virus in 42.8% of tissue samples from five fatal cases. Our results suggest that the rapid centrifugation assay may be useful for detection of dengue virus in clinical specimens.
Rodríguez  Roche
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R
Department of Virology, PAHO/WHO Collaborator Center for Viral Diseases, "Pedro Kourí" Tropical Medicine Institute, Havana, Cuba.
Alvarez
M
M
Guzmán
M G
MG
Morier
L
L
Kourí
G
G
D000330:Aedes; D000818:Animals; D002478:Cells, Cultured; D002498:Centrifugation; D003715:Dengue / Q000097:blood / Q000175:diagnosis* / Q000821:virology; D003716:Dengue Virus / Q000254:growth & development* / Q000302:isolation & purification*; D006801:Humans; D019595:Severe Dengue / Q000097:blood / Q000175:diagnosis* / Q000821:virology; D014773:Virology / Q000379:methods*; D014776:Virus Cultivation