Cytosensor techniques for examining signal transduction of neurohormones.
This review describes the principles of microphysiometry and how they can be applied, using the Cytosensor, to the investigation of the signal transduction mechanisms activated by both G-protein and non-G-protein coupled hormone and neuropeptide receptors. The use of the Cytosensor to study desensitisation and cross-talk is also discussed, as are the benefits and limitations of this technique.
Smart
D
D
SmithKline Beecham Pharmaceuticals, Neuroscience Research, Harlow, Essex, UK.
Wood
M D
MD
D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation; D002460:Cell Line; D019204:GTP-Binding Proteins / Q000378:metabolism; D006728:Hormones / Q000378:metabolism; D006801:Humans; D008024:Ligands; D009479:Neuropeptides / Q000378:metabolism; D015398:Signal Transduction; D013329:Structure-Activity Relationship
D006728:Hormones; D008024:Ligands; D009479:Neuropeptides; D019204:GTP-Binding Proteins