title: Bovine herpesvirus 1 glycoprotein G is required for viral growth by cell-to-cell infection.
abstract: The bovine herpesvirus 1 (BHV-1) US4 gene encodes glycoprotein G (gG), which is conserved in the majority of alphaherpesviruses. In order to identify the role of BHV-1 gG in the viral infection cycle, a gG minus BHV-1 mutant and its gG-positive revertant were constructed and their growth characteristics in Madin-Darby bovine kidney (MDBK) cells were compared. The gG minus mutant formed smaller plaques than the gG-positive BHV-1 in MDBK cells. When a monolayer culture of MDBK cells was infected with BHV-1 at a low multiplicity of infection and overlaid with semi-solid growth medium, under which adsorption of the mature virion released in the medium was inhibited, gG-positive BHV-1 multiplied, while the growth of the gG negative BHV-1 was severely inhibited. These data suggest that BHV-1 gG functions in direct cell-to-cell transmission mechanism of BHV-1 in tissue culture.
authors.affiliation: Department of Global Agricultural Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, 113-8657, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nakamichi
authors.forename: K
authors.initials: K
authors.lastname: Ohara
authors.forename: D
authors.initials: D
authors.lastname: Kuroki
authors.forename: H
authors.initials: H
authors.lastname: Otsuka
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D007242:Herpesvirus 1, Bovine / Q000235:genetics / Q000254:growth & development*; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D014764:Viral Proteins; C046998:bovine herpesvirus type-1 glycoproteins