Comparison of poultry processing equipment surfaces for susceptibility to bacterial attachment and biofilm formation.
During processing of poultry meat products, broiler carcasses come in contact with many solid surfaces. Bacteria from the carcasses can attach to wet equipment surfaces, form biofilms, and provide a source of cross-contamination for subsequent carcasses. In this study an array of common equipment surface materials was compared for susceptibility to bacterial attachment and biofilms. To model mixed microbial populations relevant to poultry processing, samples were taken directly from the processing line and exposed to the surface materials. Whole carcasses were rinsed with phosphate-buffered saline (100 mL), and the rinse was diluted in nutrient broth. Absorbance values (412 nm) of the suspensions at varying dilutions containing test surfaces were compared hourly with controls without test surfaces. The kinetics of bacterial attachment and biofilm formation on test surfaces were determined under the influence of pH, time, and bacterial cell density, and the elemental composition of the surface materials was determined by energy-dispersive X-ray analysis. Our results showed that surfaces vary in affinity for bacterial attachment and biofilm formation. Analysis by spectrophotometry and scanning electron microscopy confirmed that attachment to stainless steel, polyethylene, and belting was not significantly different from controls. Attachment to picker-finger rubber was significantly less than attachment to stainless steel and the other surfaces. In fact, picker-finger rubber inhibits bacterial contamination. An increased understanding of bacterial attachment and biofilm formation will assist in the development of interventions to counteract these processes and, thereby, enhance plant sanitation and pathogen control.
Arnold
J W
JW
US Department of Agriculture, Agricultural Research Service, Russell Research Center, Athens, Georgia 30604, USA. jarnold@ars.usda.gov
Silvers
S
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D000818:Animals; D001422:Bacterial Adhesion; D018441:Biofilms; D015169:Colony Count, Microbial; D005511:Food Handling / Q000295:instrumentation*; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D011200:Poultry / Q000382:microbiology*; D013053:Spectrophotometry; D013232:Steel; D013499:Surface Properties; D013997:Time Factors
D013232:Steel