Degradation of outer membrane protein A in Escherichia coli killing by neutrophil elastase.
In determining the mechanism of neutrophil elastase (NE)-mediated killing of Escherichia coli, we found that NE degraded outer membrane protein A (OmpA), localized on the surface of Gram-negative bacteria. NE killed wild-type, but not OmpA-deficient, E. coli. Also, whereas NE-deficient mice had impaired survival in response to E. coli sepsis, as compared to wild-type mice, the presence or absence of NE had no influence on survival in response to sepsis that had been induced with OmpA-deficient E. coli. These findings define a mechanism of nonoxidative bacterial killing by NE and point to OmpA as a bacterial target in host defense.
Belaaouaj
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A
Department of Pediatrics, Washington University School of Medicine, St. Louis, MO 63110, USA. belaaouaja@msnotes.wustl.edu
Kim
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KS
Shapiro
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SD
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D001770:Blood Bactericidal Activity; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D015169:Colony Count, Microbial; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D004927:Escherichia coli Infections / Q000276:immunology / Q000382:microbiology; D006801:Humans; D019272:Leukocyte Elastase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008854:Microscopy, Electron; D009154:Mutation; D009504:Neutrophils / Q000201:enzymology* / Q000382:microbiology; D010588:Phagosomes / Q000201:enzymology / Q000382:microbiology; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins / Q000494:pharmacology; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology
D001425:Bacterial Outer Membrane Proteins; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins; D015842:Serine Proteinase Inhibitors; C078167:OMPA outer membrane proteins; D019272:Leukocyte Elastase