Comparison of aqueous and non-aqueous capillary electrochromatography for the separation of basic solutes.
The analysis of basic compounds by capillary electrochromatography (CEC) on silica-based materials using conventional HPLC stationary phases has failed to address the problem of severe peak tailing and non-reproducible chromatography. Several new generation stationary phases were evaluated using aqueous and non-aqueous mobile phases. The best results were obtained in the aqueous mode using Waters Symmetry Shield RP-8, a material in which the residual silanol groups were shielded by an octylcarbamate function. For comparison, experiments were carried out using unmodified silica.
Smith
N W
NW
Imperial College of Science, Technology and Medicine, Zeneca/SmithKline Beecham Centre for Analytical Sciences, Department of Chemistry, South Kensington, London, UK. n.w.smith@ic.ac.uk
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D004364:Pharmaceutical Preparations / Q000302:isolation & purification; D013696:Temperature; D014867:Water / Q000737:chemistry
D004364:Pharmaceutical Preparations; D014867:Water