Preparation of specific antisera to digoxin by using digoxin C-3' and C-3" hemisuccinate-bovine serum albumin conjugates.
The preparation and antigenic properties of digoxin C-3' and C-3" hemisuccinate-bovine serum albumin (BSA) conjugates are described. The antisera were prepared by immunizing rabbits with each of the digoxin-BSA conjugates, and properties of the antisera were characterized by RIA with 3H-labeled digoxin. The antidigoxin antiserum from immunization with digoxin 3'-hemisuccinate-BSA conjugate possessed high specificity for digoxin, exhibiting fairly low cross-reactions with dihydrodigoxin (2.1%), digoxigenin monodigitoxoside (0.9%), digoxigenin bisdigitoxoside (0.6%), and digoxigenin (0.1%).
Ikeda
Y
Y
Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, Japan. y-ikeda@hokuriku-u.ac.jp
Fujii
Y
Y
D003429:Cross Reactions; D004077:Digoxin / Q000276:immunology*; D007106:Immune Sera; D011863:Radioimmunoassay; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000276:immunology; D013386:Succinates / Q000737:chemistry* / Q000276:immunology
D007106:Immune Sera; D013386:Succinates; D012710:Serum Albumin, Bovine; D004077:Digoxin