title: The manganese cation disrupts membrane dynamics along the secretory pathway.
abstract: The endoplasmic reticulum and Golgi apparatus play key roles in regulating the folding, assembly, and transport of newly synthesized proteins along the secretory pathway. We find that the divalent cation manganese disrupts the Golgi apparatus and endoplasmic reticulum (ER). The Golgi apparatus is fragmented into smaller dispersed structures upon manganese treatment. Golgi residents, such as TGN46, beta1,4-galactosyltransferase, giantin, and GM130, are still segregated and partitioned correctly into smaller stacked fragments in manganese-treated cells. The mesh-like ER network is substantially affected and peripheral ER elements are collapsed. These effects are consistent with manganese-mediated inhibition of motor proteins that link membrane organelles along the secretory pathway to the cytoskeleton. This divalent cation thus represents a new tool for studying protein secretion and membrane dynamics along the secretory pathway.
authors.affiliation: Department of Biochemistry, University of Dundee, Dundee, DD1 5EH, United Kingdom.
authors.forename: M C
authors.initials: MC
authors.lastname: Towler
authors.forename: A R
authors.initials: AR
authors.lastname: Prescott
authors.forename: J
authors.initials: J
authors.lastname: James
authors.forename: J M
authors.initials: JM
authors.lastname: Lucocq
authors.forename: S
authors.initials: S
authors.lastname: Ponnambalam
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001692:Biological Transport / Q000502:physiology; D015415:Biomarkers; D019556:COS Cells; D002412:Cations / Q000494:pharmacology; D003594:Cytoplasmic Granules / Q000187:drug effects* / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism* / Q000648:ultrastructure; D006056:Golgi Apparatus / Q000378:metabolism* / Q000648:ultrastructure; D006367:HeLa Cells; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism / Q000648:ultrastructure; D008345:Manganese / Q000494:pharmacology*; D008854:Microscopy, Electron; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism
chemical_list: D015415:Biomarkers; D002412:Cations; D008345:Manganese; D000255:Adenosine Triphosphate