Projection pattern of vomeronasal neurons to the accessory olfactory bulb in goats.
Goats have a well-developed vomeronasal (VN) system and exhibit pheromone-induced reproductive facilitation, but there are no reports on the projection pattern of VN neurons in this species. Rodent, guinea pig and opossum accessory olfactory bulbs (AOBs) have been shown to have a segregated pattern of projection of the VN neurons, which express the two alpha-subtypes of the G-protein, namely Gi2 and Go, to the rostral and caudal regions of the AOB, respectively. In this study we investigated the projection pattern of VN nerve terminals by immunocytochemical staining of the goat vomeronasal organ (VNO) and the AOB with antibodies to Gi2 and Go. Gi2-immunoreactivity was found on the luminal surface of the sensory epithelium of the VNO, and in the VN nerve and glomerular layer throughout the AOB. On the other hand, Go-immunoreactivity was not identified in either the VNO or the VN nerve layer of the AOB. These results indicate that the projection pattern of VN neurons from the VNO to the AOB in the goat is considerably different from that in rodents which show a distinct segregated pattern.
Takigami
S
S
Department of Anatomy and Embryology, Tokyo Metropolitan Institute for Neuroscience, 2-6 Musashidai, Fuchu, Tokyo 183-8526, Japan.
Mori
Y
Y
Ichikawa
M
M
D000818:Animals; D015153:Blotting, Western; D005260:Female; D006041:Goats; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000502:physiology*; D009830:Olfactory Bulb / Q000166:cytology*; D051381:Rats; D019147:Vomeronasal Organ / Q000166:cytology*