title: Growth of Steptomyces hygroscopicus in rotating-wall bioreactor under simulated microgravity inhibits rapamycin production.
abstract: Growth of Streptomyces hygroscopicus under conditions of simulated microgravity in a rotating-wall bioreactor resulted in a pellet form of growth, lowered dry cell weight, and inhibition of rapamycin production. With the addition of Teflon beads to the bioreactor, growth became much less pelleted, dry cell weight increased but rapamycin production was still markedly inhibited. Growth under simulated microgravity favored extracellular production of rapamycin, in contrast to a greater percentage of cell-bound rapamycin observed under normal gravity conditions.
authors.affiliation: Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.
authors.forename: A
authors.initials: A
authors.lastname: Fang
authors.forename: D L
authors.initials: DL
authors.lastname: Pierson
authors.forename: S K
authors.initials: SK
authors.lastname: Mishra
authors.forename: A L
authors.initials: AL
authors.lastname: Demain
mesh_terms: D019149:Bioreactors; D003470:Culture Media; D020123:Sirolimus / Q000378:metabolism*; D013302:Streptomyces / Q000254:growth & development* / Q000378:metabolism; D014893:Weightlessness
chemical_list: D003470:Culture Media; D020123:Sirolimus