title: Production and characterization of antibodies directed against the human melatonin receptors Mel-1a (mt1) and Mel-1b (MT2).
abstract: We report the production of polyclonal antibodies directed against the human melatonin receptors Mel-1a (mt1) and Mel-1b (MT2) by means of antigenic synthetic peptides with sequences unique to these proteins. Immunostaining on NIH3T3 cells stably transfected with Mel-1a and Mel-1b cDNA gave intense reactions. Neither the preimmune serum nor cross-tested antisera showed any reactivity. These polyclonal antibodies will be essential immunocytochemical tools to study the human melatonin receptors distribution at subcellular level.
authors.affiliation: Department of Pharmacology, University of Milan, Italy. andreazzolid@mail.ncifcrf.gov
authors.forename: D
authors.initials: D
authors.lastname: Angeloni
authors.forename: R
authors.initials: R
authors.lastname: Longhi
authors.forename: F
authors.initials: F
authors.lastname: Fraschini
mesh_terms: D016475:3T3 Cells; D000818:Animals; D000917:Antibody Formation; D006801:Humans; D051379:Mice; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D018160:Receptors, Cytoplasmic and Nuclear / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D044094:Receptors, Melatonin
chemical_list: D011956:Receptors, Cell Surface; D018160:Receptors, Cytoplasmic and Nuclear; D044094:Receptors, Melatonin