title: Cross-protection and reassortment studies with avian H2 influenza viruses.
abstract: In order to assess the degree of immune cross-protection among avian H2 influenza virus strains, mice were immunised with beta-propiolactone-inactivated virus preparations and infected intranasally with mouse-adapted variant of A/Black Duck/New Jersey/1580/78 (H2N3) strain. The experiments with 11 avian H2 strains revealed that both Eurasian and American H2 avian influenza viruses exhibit either high or moderate degree of cross-protection. The grouping of the strains in accordance with their cross-protection efficiency does not coincide with H2 phylogenetic branches. Several reassortant clones were obtained with the use of A/Pintail Duck/Primorie/695/76 (H2N3) strain and high-yield X-67 reassortant as parent viruses, among them a high-yield H2N3 reassortant. Taking into account the data on cross-protection among avian H2 strains, the high-yield H2N3 reassortant may be regarded as a prototype strain to be used for the preparation of killed vaccines in the case of a new appearance of avian H2 haemagglutinin in circulation in humans.
authors.affiliation: The DI Ivanovsky Institute of Virology RAMS, Moscow, Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Kaverin
authors.forename: Y A
authors.initials: YA
authors.lastname: Smirnov
authors.forename: E A
authors.initials: EA
authors.lastname: Govorkova
authors.forename: I A
authors.initials: IA
authors.lastname: Rudneva
authors.forename: A K
authors.initials: AK
authors.lastname: Gitelman
authors.forename: A S
authors.initials: AS
authors.lastname: Lipatov
authors.forename: N L
authors.initials: NL
authors.lastname: Varich
authors.forename: S S
authors.initials: SS
authors.lastname: Yamnikova
authors.forename: N V
authors.initials: NV
authors.lastname: Makarova
authors.forename: R G
authors.initials: RG
authors.lastname: Webster
authors.forename: D K
authors.initials: DK
authors.lastname: Lvov
mesh_terms: D000818:Animals; D002642:Chick Embryo; D003429:Cross Reactions; D007114:Immunization; D009980:Influenza A virus / Q000235:genetics* / Q000276:immunology* / Q000472:pathogenicity; D007252:Influenza Vaccines / Q000276:immunology*; D051379:Mice; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000517:prevention & control*; D016865:Reassortant Viruses / Q000235:genetics / Q000276:immunology*; D014613:Vaccines, Attenuated / Q000276:immunology
chemical_list: D007252:Influenza Vaccines; D014613:Vaccines, Attenuated