title: Axonal regeneration of retinal ganglion cells depending on the distance of axotomy in adult hamsters.
abstract: OBJECTIVE
  To examine the relationship between the distance of axotomy and axonal regeneration of injured retinal ganglion cells (RGCs) systematically and the effect of a predegenerated (pretransected or precrushed) peripheral nerve (PN) graft on axonal regeneration of RGCs axotomized at a definite distance (0.5 mm from the optic disc) in comparison with a normal PN graft.
  
  
  METHODS
  The optic nerve (ON) was transected intraorbitally at 0.5, 1, 1.5, 2, or 3 mm or intracranially at 6 to 8 mm from the optic disc, and a PN graft was transplanted onto the ocular ON stump in adult hamsters. Four weeks after grafting, the number of RGCs regenerating their injured axons into the PN graft was investigated in all animals.
  
  
  RESULTS
  The number of regenerating RGCs decreased significantly when the distance of axotomy increased from 0.5 to 7 mm. A precrushed PN graft was shown to enhance more injured RGCs to regenerate axons than a normal or pretransected PN graft.
  
  
  CONCLUSIONS
  The distance of axotomy on the ON of adult hamsters is critical in determining the number of regenerating RGCs. Thus, experimental strategies to repair the damaged ON by PN transplantation is to attach a precrushed PN graft as close to the optic disc as possible to obtain optimal axonal regeneration of the axotomized RGCs.
authors.affiliation: Department of Anatomy, The University of Hong Kong, China.
authors.forename: S W
authors.initials: SW
authors.lastname: You
authors.forename: K F
authors.initials: KF
authors.lastname: So
authors.forename: H K
authors.initials: HK
authors.lastname: Yip
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology*; D019771:Axotomy; D002452:Cell Count; D002470:Cell Survival; D006224:Cricetinae; D005260:Female; D008647:Mesocricetus; D009416:Nerve Regeneration; D009900:Optic Nerve / Q000033:anatomy & histology*; D012165:Retinal Ganglion Cells / Q000502:physiology*