Pyruvate dehydrogenase activity and energy metabolite levels following bilateral common carotid artery occlusion in rat brain.
The influence of chronic cerebral hypoperfusion on cerebral energy metabolism was studied. The bilateral common carotid arteries of Wistar rats were occluded for 0, 2, 7, and 28 days. Cerebral energy metabolism was evaluated by assaying adenosine triphosphate (ATP), phosphocreatine (PCr), and lactate levels and measuring pyruvate dehydrogenase (PDH) activity (each time point, n = 6). Pathological changes were assessed light-microscopically by Klüver-Barrera staining and immunohistochemical labeling for astroglia (each time point, n = 3). There were no changes in ATP and PCr levels or PDH activity; there was slight but significant transient lactate accumulation at 2 days. Myelin pallor and increase in immuno-reactive astroglia were only observed at 28 days. These results indicate that chronic cerebral hypoperfusion induces delayed white matter changes in the corpus callosum of rat brain, but does not affect energy production.
Ueda
M
M
Second Department of Internal Medicine, Nippon Medical School, Tokyo, Japan. ueda@nms.ac.jp
Muramatsu
H
H
Kamiya
T
T
Muramatsu
A
A
Mori
T
T
Terashi
A
A
Katayama
Y
Y
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000098:blood supply* / Q000201:enzymology / Q000378:metabolism*; D002545:Brain Ischemia / Q000201:enzymology / Q000378:metabolism; D017536:Carotid Artery, Common / Q000502:physiology; D002540:Cerebral Cortex / Q000098:blood supply / Q000378:metabolism; D003337:Corpus Callosum / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D003342:Corpus Striatum / Q000098:blood supply / Q000378:metabolism; D004734:Energy Metabolism / Q000502:physiology*; D019344:Lactic Acid / Q000378:metabolism; D008297:Male; D010725:Phosphocreatine / Q000378:metabolism; D011768:Pyruvate Dehydrogenase Complex / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D011768:Pyruvate Dehydrogenase Complex; D010725:Phosphocreatine; D019344:Lactic Acid; D000255:Adenosine Triphosphate