title: Palm vitamin E is comparable to alpha-tocopherol in maintaining bone mineral density in ovariectomised female rats.
abstract: Vitamin E has been shown to affect bone metabolism. In this study we determined the effects of palm vitamin E and alpha-tocopherol on bone metabolism. Sprague-Dawley female rats fed with normal rat chow were divided into 4 groups and supplemented with either palm vitamin E 30 mg/kg rat weight, palm vitamin E 60 mg/kg rat weight or alpha-tocopherol 30 mg/kg rat weight. One group was not supplemented. Half of these rats were ovariectomised before supplementation was given for 10 months. As expected, bone mineral density of the ovariectomised rats fed on normal rat chow diet was lower compared to the intact rats. However, these changes were not seen in the supplemented group of rats. Both intact and ovariectomised rats supplemented with palm vitamin E 30 mg/kg rat weight had a lower bone calcium content in both femoral and vertebral bones whilst rats fed palm vitamin E 60 mg/kg rat weight or alpha-tocopherol 30 mg/kg rat weight were able to maintain bone calcium content. Alkaline phosphatase activity was elevated in ovariectomised rats supplemented with palm vitamin E 30 mg/kg rat weight and alpha-tocopherol 30 mg/kg rat weight compared to the intact rats. Alpha-tocopherol also reduced the activity of tartrate-resistant acid phosphatase post-ovariectomy. These findings indicate that both palm vitamin E and alpha-tocopherol maintained bone mineral density in ovariectomised rats but caused conflicting effects on bone calcium content. Further study is needed in order to determine the mechanisms involved.
authors.affiliation: Department of Pharmacology, Faculty of Medicine, Universiti Kebangsaan, Kuala Lumpur, Malaysia.
authors.forename: M
authors.initials: M
authors.lastname: Norazlina
authors.forename: S
authors.initials: S
authors.lastname: Ima-Nirwana
authors.forename: M T
authors.initials: MT
authors.lastname: Gapor
authors.forename: B A
authors.initials: BA
authors.lastname: Khalid
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D015519:Bone Density / Q000187:drug effects*; D001842:Bone and Bones / Q000201:enzymology; D002118:Calcium / Q000378:metabolism; D005260:Female; D007527:Isoenzymes / Q000378:metabolism; D010052:Ovariectomy; D000073878:Palm Oil; D010938:Plant Oils / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000071681:Tartrate-Resistant Acid Phosphatase; D014810:Vitamin E / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D007527:Isoenzymes; D010938:Plant Oils; D014810:Vitamin E; D000073878:Palm Oil; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D002118:Calcium