title: Anhydrobiotic engineering of gram-negative bacteria.
abstract: Anhydrobiotic engineering aims to improve desiccation tolerance in living organisms by adopting the strategies of anhydrobiosis. This was achieved for Escherichia coli and Pseudomonas putida by osmotic induction of intracellular trehalose synthesis and by drying from trehalose solutions, resulting in long-term viability in the dried state.
authors.affiliation: Institute of Biotechnology, University of Cambridge, Cambridge CB2 1QT, United Kingdom.
authors.forename: A
authors.initials: A
authors.lastname: García De Castro
authors.forename: H
authors.initials: H
authors.lastname: Bredholt
authors.forename: A R
authors.initials: AR
authors.lastname: Strøm
authors.forename: A
authors.initials: A
authors.lastname: Tunnacliffe
mesh_terms: D001709:Biotechnology / Q000379:methods; D015169:Colony Count, Microbial; D003890:Desiccation; D004926:Escherichia coli / Q000254:growth & development* / Q000378:metabolism; D005612:Freeze Drying; D009997:Osmotic Pressure; D016958:Pseudomonas putida / Q000254:growth & development* / Q000378:metabolism; D014199:Trehalose / Q000378:metabolism*
chemical_list: D014199:Trehalose