Neurofilament H immunoreaction in oligodendrogliomas as demonstrated by a new polyclonal antibody.
We have characterized a new polyclonal antibody against heavy chain (H) of neurofilament which can be used to demonstrate neurofilament H in normal brain tissue and oligodendroglioma cells immunocytochemically and immunochemically. Using this antibody we found neurofilament H-immunoreactive tumor cells in 13 oligodendrogliomas (6 WHO grade II, 7 WHO grade III) out of 84 oligodendrogliomas investigated (59 WHO grade II and 25 WHO grade III). Double immunolabeling and confocal laser scanning microscopy showed colocalization of neurofilament H and glial fibrillary acidic protein in certain oligodendroglioma cells. Colocalization of neurofilament and synaptophysin was observed only rarely. The results support the notion that oligodendrogliomas consists of a heterogeneous cell population displaying various stages of differentiation and dedifferentiation. The occurrence of neurofilament H-immunoreactive tumor cells in oligodendrogliomas is not related to the survival of the patients.
Dehghani
F
F
Johann Wolfgang Goethe-Universität, Frankfurt/Main, Germany.
Maronde
E
E
Schachenmayr
W
W
Korf
H W
HW
D000293:Adolescent; D000818:Animals; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D018613:Microscopy, Confocal; D008875:Middle Aged; D016900:Neurofilament Proteins / Q000378:metabolism*; D009837:Oligodendroglioma / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D016019:Survival Analysis
D005904:Glial Fibrillary Acidic Protein; D016900:Neurofilament Proteins; C051774:neurofilament protein H