Antenatal glucocorticoids alter premature newborn lamb neuroendocrine and endocrine responses to hypoxia.
Glucocorticoids are administered for preterm labor to improve postnatal adaptation. We assessed the effect of antenatal betamethasone (Beta) treatment on preterm newborn lamb neuroendocrine [catecholamine, arginine vasopressin (AVP)] and endocrine [triiodothyronine (T(3)), ANG II, and atrial natriuretic factor (ANF)] adaptive responses following delivery and a hypoxic challenge. Beta treatment included direct fetal injection at 0.2 (F(0.2); n = 8) or 0.5 (F(0.5); n = 7) mg/kg estimated fetal body weight or maternal injection with 0.2 (n = 8) or 0.5 mg/kg (M(0.5); n = 8). Control animals received fetal and maternal intramuscular injections of saline (n = 8). After 24 h, lambs were delivered by cesarean section, surfactant treated, and ventilated for 4 h. Relative to the control lambs, 3 h after delivery, there was a marked suppression of plasma cortisol, epinephrine, norepinephrine, and ANG II levels and elevated plasma T(3) and ANF levels, systolic blood pressure, and left ventricular contractility (dP/dt; F(0.5) and M(0.5)) values in F(0.5) and both maternal Beta-treated groups. However, Beta treatment augmented the cardiac output, cortisol, norepinephrine, AVP, and ANF responses to 20 min of hypoxia (PO(2) = 25-30 mmHg). We concluded that short-term (24 h) antenatal glucocorticoid exposure 1) alters preterm newborn postnatal blood pressure regulation in the face of marked depression of plasma cortisol, catecholamine, and ANG II levels and 2) augments the postnatal neuroendocrine and endocrine responses to a hypoxic challenge.
Ervin
M G
MG
Department of Biology, Middle Tennessee State University, Murfreesboro, Tennessee 37132, USA. mervin@mtsu.edu
Padbury
J F
JF
Polk
D H
DH
Ikegami
M
M
Berry
L M
LM
Jobe
A H
AH
D000804:Angiotensin II / Q000097:blood; D000818:Animals; D000831:Animals, Newborn; D001127:Arginine Vasopressin / Q000097:blood; D009320:Atrial Natriuretic Factor / Q000097:blood; D001623:Betamethasone / Q000494:pharmacology*; D001784:Blood Gas Analysis; D001794:Blood Pressure / Q000187:drug effects; D004837:Epinephrine / Q000097:blood; D005260:Female; D005865:Gestational Age; D005938:Glucocorticoids / Q000494:pharmacology*; D006339:Heart Rate / Q000187:drug effects; D006854:Hydrocortisone / Q000097:blood; D006863:Hydrogen-Ion Concentration; D000860:Hypoxia / Q000097:blood / Q000503:physiopathology*; D009490:Neurosecretory Systems / Q000187:drug effects* / Q000502:physiology*; D009638:Norepinephrine / Q000097:blood; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D012756:Sheep; D014284:Triiodothyronine / Q000097:blood*; D014469:Umbilical Arteries
D005938:Glucocorticoids; D014284:Triiodothyronine; D000804:Angiotensin II; D001127:Arginine Vasopressin; D009320:Atrial Natriuretic Factor; D001623:Betamethasone; D006854:Hydrocortisone; D009638:Norepinephrine; D004837:Epinephrine