title: In vitro purgative effect of Ruellia praetermissa. Sceinf.ex.Lindau (Acanthaceae).
abstract: Methanol, ethylacetate and aqueous extracts of Ruellia praetermissa initiated spontaneous contractions in the quiescent and increased contraction on the electrically stimulated ileal strip at a concentration of 30 microg/ml. The extracts produced concentration-related contractions both in amplitude and tone up till 750 microg/ml with IC(50) of 360 microg/ml (methanol extract), 425 microg/ml (ethylacetate extract) and 540 microg/ml (aqueous extract). Acetylcholine also produced a concentration-related (IC(50)=18 microg/ml) contractions of the isolated ileum. Atropine in concentrations of 3.4 x 10(-6)-3 x 10(-3) microg/ml antagonized progressively the response of the isolated ileum to acetylcholine (32 x 10(-2) microg/ml) and the methanol extract (650 microg/ml) induced contractions suggesting a mode of action via cholinergic system. Luteolin and apigenin and iridoid glucosides (taxiphilin and 8-epi-deoyganic acid) might be responsible at least in part for the observed effect.
authors.affiliation: Institute of Pharmaceutical Biology, University of Munich, Butenandtstr. 5-13, D-81375 Munich, Germany.
authors.forename: A M
authors.initials: AM
authors.lastname: Salah
authors.forename: A B
authors.initials: AB
authors.lastname: Dongmo
authors.forename: A
authors.initials: A
authors.lastname: Kamanyi
authors.forename: M
authors.initials: M
authors.lastname: Bopelet
authors.forename: W
authors.initials: W
authors.lastname: Vierling
authors.forename: H
authors.initials: H
authors.lastname: Wagner
mesh_terms: D000818:Animals; D002163:Cameroon; D002400:Cathartics / Q000737:chemistry / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D000431:Ethanol; D005260:Female; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects; D066298:In Vitro Techniques; D008297:Male; D016488:Medicine, African Traditional; D000432:Methanol; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry*; D012997:Solvents; D014867:Water
chemical_list: D002400:Cathartics; D010936:Plant Extracts; D012997:Solvents; D014867:Water; D000431:Ethanol; D000432:Methanol