[Construction by using retrovirus vector of recombinant with synthetic bradykinin "gene" for investigations of human gene expression in mammalian cells].
The synthetic gene of bradykinin was built into the retrovirus vector pPS-3-neo under the guidance of LTR promotor, followed by pPS-3-neo (brd) vector transfection of strain 293 cells. The physiological activity of the expressed bradykinin was tested on cultured neonatal rat cardiomyocytes. The culture medium of strain 293 cells transferred by pPS-3-neo (brd) produces a positive chronotropic effect that is directly related to the time parameters of preparation of recombinant bradykinin, which are comparable with the curve of chronotropic effect of synthetic bradykinin at concentrations of 10(-17) to 10(-16) M. The control of bradykinin "gene" expression was due to the lack of chronotropic responses of cardiomyocytes to the kinin receptor blocker parmidine and the transfection of strain 293 cells with the retrovirus vector without bradykinin "gene".
Neverova
M E
ME
Budanov
A V
AV
Adamskaia
E I
EI
Prasolov
V S
VS
Kalinin
M E
ME
D000818:Animals; D000831:Animals, Newborn; D001920:Bradykinin / Q000235:genetics* / Q000494:pharmacology; D002472:Cell Transformation, Viral / Q000187:drug effects / Q000235:genetics*; D002478:Cells, Cultured / Q000821:virology; D015870:Gene Expression / Q000187:drug effects / Q000235:genetics*; D005822:Genetic Vectors / Q000494:pharmacology*; D006321:Heart / Q000821:virology; D006801:Humans; D000960:Hypolipidemic Agents / Q000494:pharmacology; D007668:Kidney / Q000166:cytology* / Q000187:drug effects / Q000821:virology; D009206:Myocardium / Q000166:cytology; D011727:Pyridinolcarbamate / Q000494:pharmacology; D051381:Rats; D011994:Recombinant Proteins; D012190:Retroviridae / Q000235:genetics*; D014162:Transfection / Q000379:methods*
D000960:Hypolipidemic Agents; D011994:Recombinant Proteins; D011727:Pyridinolcarbamate; D001920:Bradykinin