Direct evidence for involvement of NF-kappaB in transcriptional activation of tumor necrosis factor by a spirochetal lipoprotein.
Variable major lipoprotein (Vmp) is a major tumor necrosis factor (TNF)-inducing component of Borrelia recurrentis, the agent of louse-borne relapsing fever. B. recurrentis Vmp rapidly stimulates nuclear translocation of NF-kappaB and proinflammatory cytokine gene expression in the human monocyte-like cell line MonoMac 6. By overexpressing disabled mutant IkappaBalpha in MonoMac 6 cells cotransfected with a reporter gene, we provide evidence that NF-kappaB is essential for the transcriptional activation of TNF in this system.
Udalova
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IA
Molecular Infectious Disease Group, Institute of Molecular Medicine, The John Radcliffe Hospital, Oxford OX3 9DS, United Kingdom. iudalova@molbiol.ox.ac.uk
Vidal
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V
Scragg
I G
IG
Kwiatkowski
D
D
D001426:Bacterial Proteins / Q000502:physiology*; D001898:Borrelia / Q000502:physiology*; D002460:Cell Line; D016207:Cytokines / Q000235:genetics; D006801:Humans; D016328:NF-kappa B / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D015533:Transcriptional Activation; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
D001426:Bacterial Proteins; D016207:Cytokines; D016328:NF-kappa B; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; C116109:VMPA1 protein, Borrelia recurrentis