title: Determination of sugar compounds in olive plant extracts by anion-exchange chromatography with pulsed amperometric detection.
abstract: We describe a chromatographic method that uses isocratic elution and pulsed amperometric detection to determine soluble carbohydrates in plant tissues. Such a method provides a rapid and convenient means to obtain a complete profile of the sugar components of leaves and roots from olive (Olea europaea L. cv. Coratina) plants. A simple purification of plant extracts using pure water was developed, which is far less time-consuming and retains a high level of accuracy. Excellent separation of myo-inositol, galactinol, mannitol, galactose, glucose, fructose, sucrose, raffinose, and stachyose was achieved with an anion-exchange column and 12 mM NaOH spiked with 1 mM barium acetate as an eluent. At a flow rate of 1.0 mL/min, the time of analysis was less than 25 min, and repeatability of the method on the order of 2.2% as RSD or better for retention times and lower than 5.2% for peak areas. Recoveries approximated 100% (range 97.2-104.5%), and the method provided good precision with a coefficient of variation which ranged between 0.9 and 3.3%. Among identified carbohydrates extracted from leaves and roots of olive plants, glucose and mannitol were major compounds. Their molar ratio was estimated to be 1.2+/-0.1 and 2.2+/-0.3 for olive leaves and roots, respectively. The occurrence of soluble galactinol in plant tissues was also validated.
authors.affiliation: Dipartimento di Chimica, Università degli Studi della Basilicata, Potenza, Italy. cataldi@unibas.it
authors.forename: T R
authors.initials: TR
authors.lastname: Cataldi
authors.forename: G
authors.initials: G
authors.lastname: Margiotta
authors.forename: L
authors.initials: L
authors.lastname: Iasi
authors.forename: B
authors.initials: B
authors.lastname: Di Chio
authors.forename: C
authors.initials: C
authors.lastname: Xiloyannis
authors.forename: S A
authors.initials: SA
authors.lastname: Bufo
mesh_terms: D000837:Anion Exchange Resins; D002241:Carbohydrates / Q000032:analysis*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004563:Electrochemistry; D005638:Fruit / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry
chemical_list: D000837:Anion Exchange Resins; D002241:Carbohydrates; D010936:Plant Extracts