Somatic mutation of PTEN in vulvar cancer.
PTEN, a candidate tumor suppressor gene located at chromosome 10q23.3, has been shown to be mutated in approximately 40% of endometrial cancers. Such mutations have also been identified in endometrial hyperplasia, indicating that inactivation of the PTEN tumor suppressor gene is an early event in the genesis of some endometrial cancers. In this study, we have extended the analysis of PTEN in gynecological cancer to include adenocarcinoma of the cervix and vulvar carcinomas. Microdissected tissue (including normal tissues), preneoplastic, and neoplastic lesions were analyzed from 9 patients with cervical cancer and 10 patients with vulvar cancer. Only 1 cervical adenocarcinoma displayed a PTEN mutation. In contrast, five of eight vulvar carcinomas studied harbored PTEN mutations. Alterations were identified in carcinoma in situ as well as squamous cell carcinoma of the vulva. In two patients, PTEN mutations were identified in mucosal regions with mild or focal dysplasia. These results suggest that PTEN is frequently altered in vulvar carcinomas and can be found associated with early dysplastic changes in vulvar mucosa.
Holway
A H
AH
Department of Research, Robert E. Wise M. D. Research and Education Institute, Burlington, Massachusetts 01805, USA.
Rieger-Christ
K M
KM
Miner
W R
WR
Cain
J W
JW
Dugan
J M
JM
Pezza
J A
JA
Silverman
M L
ML
Shapter
A
A
McLellan
R
R
Summerhayes
I C
IC
D000230:Adenocarcinoma / Q000235:genetics; D002278:Carcinoma in Situ / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics; D016889:Endometrial Neoplasms / Q000235:genetics; D005260:Female; D006801:Humans; D006965:Hyperplasia / Q000235:genetics; D009154:Mutation; D051059:PTEN Phosphohydrolase; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics*; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D012680:Sensitivity and Specificity; D025521:Tumor Suppressor Proteins; D002583:Uterine Cervical Neoplasms / Q000235:genetics; D014844:Vulva / Q000473:pathology; D014846:Vulvar Neoplasms / Q000235:genetics*
D025521:Tumor Suppressor Proteins; D010744:Phosphoric Monoester Hydrolases; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human