Differential cytokine mRNA expression in Dermatophagoides farinae allergen-sensitized and respiratory syncytial virus-infected mice.
The interaction between mite allergen sensitization and respiratory syncytial virus (RSV) infection at the level of cytokine mRNA expression was examined in a murine model in the present study. Primary RSV infection enhances expression of inflammatory cytokines such as IL-6, IFN-gamma, and eotaxin in the lung and upregulates the expression of Th2-like cytokines IL-10 and IL-13 in the spleen in BALB/c mice. Mite antigen-sensitized and RSV-infected (ASRSV) mice show enhanced (P < 0.05) total serum IgE compared to antigen-sensitized mice. However, the levels of viral mRNA in the lung tissues are comparable between RSV-infected and ASRSV mice. It is concluded that compartmentalization of cytokine expression following RSV infection plays a role in the augmentation of Th2-like and IgE antibody response to RSV.
Matsuse
H
H
The Joy McCann Culverhouse Airway Disease Center, Department of Internal Medicine, University of South Florida and James A. Haley VA Hospital, Tampa 33612, USA.
Behera
A K
AK
Kumar
M
M
Lockey
R F
RF
Mohapatra
S S
SS
D000485:Allergens / Q000276:immunology*; D000818:Animals; D054413:Chemokine CCL11; D019742:Chemokines, CC; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D005260:Female; D007114:Immunization; D007073:Immunoglobulin E / Q000096:biosynthesis; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D018793:Interleukin-13 / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008925:Mites / Q000276:immunology*; D012333:RNA, Messenger / Q000032:analysis; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000378:metabolism; D018418:Th2 Cells / Q000378:metabolism; D015854:Up-Regulation; D014779:Virus Replication
D000485:Allergens; C515457:Ccl11 protein, mouse; D054413:Chemokine CCL11; D019742:Chemokines, CC; D016207:Cytokines; D018793:Interleukin-13; D012333:RNA, Messenger; D016753:Interleukin-10; D007073:Immunoglobulin E; D007371:Interferon-gamma