Partitioning of xylanolitic complex from Penicillium janthinellum by an aqueous two-phase system.
This work evaluates the influences of five parameters (pH, PEG molecular mass, PEG concentration, concentration of buffer K2HPO4-KH2PO4 and NaCl concentration) on xylanolitic complex partitioning produced by P. janthinellum in aqueous two-phase systems, using a 2(5) factorial experimental design. A mathematical model to quantify the influence of these parameters was attained and statistically tested. The optimum point for total protein extraction was obtained under the following conditions: pH 7.0, PEG 10 000, 3.67% PEG, 10% potassium phosphate and 12.4% NaCl. The partition coefficient (K) value experimentally obtained was 5.25 and that predicted by the model was 5.89.
Costa
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Department of Biotechnology, Faculty of Chemical Engineering of Lorena, Lorena/SP, Brazil.
Pessoa
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Roberto
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D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008970:Molecular Weight; D010407:Penicillium / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D017680:Potassium Compounds / Q000737:chemistry; D012965:Sodium Chloride / Q000737:chemistry; D014990:Xylans / Q000378:metabolism*
D005656:Fungal Proteins; D010710:Phosphates; D017680:Potassium Compounds; D014990:Xylans; D011092:Polyethylene Glycols; D012965:Sodium Chloride; C013216:potassium phosphate