Identification of the glycoprotein 41(TM) cytoplasmic tail domains of human immunodeficiency virus type 1 that interact with Pr55Gag particles.
We investigated the protein/protein interactions that occur during human immunodeficiency virus (HIV-1) budding. We evaluated the binding to Pr55Gag particles of peptides mapping to the cytoplasmic tail of gp41TM and of host-cell proteins, in a cell-free, in vitro assay. Host-cell proteins and irrelevant viral envelope peptides did not bind. Peptides corresponding to a large central domain of the gp41TM cytoplasmic tail (93 residues) bound to Pr55Gag particles. This demonstrates that a Gag/Env interaction is responsible for the specific incorporation of the Env glycoprotein into nascent HIV-1 virions, and defines more accurately the gp41TM domain involved in this interaction.
Hourioux
C
C
Laboratoire de Virologie, EA 2639, Faculté de Médecine, Université François Rabelais, Tours, France.
Brand
D
D
Sizaret
P Y
PY
Lemiale
F
F
Lebigot
S
S
Barin
F
F
Roingeard
P
P
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D002462:Cell Membrane / Q000648:ultrastructure / Q000821:virology; D006224:Cricetinae; D015683:Gene Products, gag / Q000737:chemistry* / Q000378:metabolism*; D015700:HIV Envelope Protein gp41 / Q000737:chemistry* / Q000378:metabolism*; D015497:HIV-1 / Q000254:growth & development / Q000502:physiology* / Q000648:ultrastructure; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D010449:Peptide Mapping; D011498:Protein Precursors / Q000737:chemistry* / Q000378:metabolism*; D014162:Transfection
D015683:Gene Products, gag; D015700:HIV Envelope Protein gp41; D010446:Peptide Fragments; D011498:Protein Precursors; C059767:p55 gag precursor protein, Human immunodeficiency virus 1