title: A new look at the nuclear matrix.
abstract: The concept of the nuclear matrix, a karyoskeletal structure that serves as a support for the genome and its activities, has stimulated many studies of the association of nuclear components and functions with this structure. However, certain experimental findings are not consistent with the existence of the nuclear matrix in vivo, including our inability to visualise a corresponding structure in intact cells, the demonstrated mobility in vivo of chromatin and messenger ribonucleoprotein particles, which are claimed to be bound to the nuclear matrix, the paradoxical extractability from nuclei in low ionic strength buffers of enzymes that are found in the 2 M NaCl-insoluble matrix, and the extractability, in conditions which reproduce the intranuclear milieu, of regions of DNA (matrix or scaffold attachment regions, MAR/SARs) postulated to be bound to the nuclear matrix in vivo. This review considers the nuclear matrix model in the light of sometimes overlooked evidence that each step in its isolation may cause nuclear components to bind to it by new liaisons that do not exist in vivo. This is illustrated by experiments where nuclear-targeted green fluorescent protein is found in the nuclear matrix, and raises the possibility that MAR/SARs actually bind to DNA-binding proteins or multiprotein complexes, including replicational, transcriptional and processing machinery, and topoisomerases that are incorporated into the nuclear matrix during its preparation. Considering that the nuclear lamina forms a rigid exoskeleton, the necessity for internal skeletal structures is raised; the major roles that macromolecular crowding, phase partitioning, and charge effects are likely to play in organisation of the intranuclear space may provide new models for the compartmentalisation of proteins and functions into different nuclear domains and of chromosomes into territories.
authors.affiliation: Centre de Recherche en Cancérologie de l'Université Laval, Hôtel-Dieu Hospital, Québec, Canada. ronald.hancock@crhdq.ulaval.ca
authors.forename: R
authors.initials: R
authors.lastname: Hancock
mesh_terms: D004247:DNA / Q000378:metabolism; D015530:Nuclear Matrix / Q000201:enzymology / Q000378:metabolism / Q000648:ultrastructure*; D009994:Osmolar Concentration; D010084:Oxidation-Reduction; D012965:Sodium Chloride / Q000737:chemistry
chemical_list: D012965:Sodium Chloride; D004247:DNA