title: B-type granule containing protrusions and interconnections between amyloplasts in developing wheat endosperm revealed by transmission electron microscopy and GFP expression.
abstract: Starch granules in mature wheat endosperm show a bimodal size distribution. The formation of small starch granules in wheat endosperm cells was studied by transmission electron microscopy (TEM) and confocal laser scanning microscopy (CLSM) after expression and targeting of fluorescent protein into amyloplasts. Both techniques demonstrated the presence of protrusions emanating from A-type granules-containing amyloplasts and the presence of B-type starch granules in these evaginations. Moreover, CLSM recordings demonstrated the interconnection of the amyloplasts by these protrusions, suggesting a possible role of these protrusions in interplastid communication.
authors.affiliation: TNO department of Plant Biotechnology, Center for Phytotechnology UL/TNO, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands. Langeveld@rulbim.keidenuniv.nl
authors.forename: S M
authors.initials: SM
authors.lastname: Langeveld
authors.forename: R
authors.initials: R
authors.lastname: van Wijk
authors.forename: N
authors.initials: N
authors.lastname: Stuurman
authors.forename: J W
authors.initials: JW
authors.lastname: Kijne
authors.forename: S
authors.initials: S
authors.lastname: de Pater
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D015388:Organelles / Q000378:metabolism / Q000648:ultrastructure*; D014908:Triticum / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure*
chemical_list: D017931:DNA Primers; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins