A novel hemocyte-specific membrane protein of Sarcophaga (flesh fly).
Extensive tissue remodeling takes place during metamorphosis of holometabolous insects. It has been shown that hemocytes play crucial roles in the recognition and elimination of apoptotic cells and larval tissue fragments produced during metamorphosis. We report the immunoaffinity purification, cDNA cloning, and characterization of a prepupal hemocyte membrane protein of Sarcophaga (flesh fly) with a molecular mass of 120 kDa. This protein is a novel type I transmembrane protein with 18 repeats of an epidermal growth factor-like domain in the predicted extracellular region. Expression of the protein was restricted exclusively to prepupal hemocytes. This protein is suggested to be a scavenger receptor for tissue remodeling.
Hori
S
S
Graduate School of Pharmaceutical Sciences, The University of Tokyo, Bunkyo-ku, Japan.
Kobayashi
A
A
Natori
S
S
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001798:Blood Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004175:Diptera / Q000254:growth & development; D006434:Hemocytes / Q000737:chemistry* / Q000276:immunology; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D008675:Metamorphosis, Biological; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
C414751:120-kDa hemocyte-specific membrane protein, flesh fly; D000911:Antibodies, Monoclonal; D001798:Blood Proteins; D018076:DNA, Complementary; D008565:Membrane Proteins