title: Effect of combined supplementation with vitamin E and alpha-lipoic acid on myocardial performance during in vivo ischaemia-reperfusion.
abstract: Reactive oxygen species (ROS) contribute significantly to myocardial ischaemia-reperfusion (I-R) injury. Recently the combination of the antioxidants vitamin E (VE) and alpha-lipoic acid (alpha-LA) has been reported to improve cardiac performance and reduce myocardial lipid peroxidation during in vitro I-R. The purpose of these experiments was to investigate the effects of VE and alpha-LA supplementation on cardiac performance, incidence of dysrhythmias and biochemical alterations during an in vivo myocardial I-R insult. Female Sprague-Dawley rats (4-months old) were assigned to one of the two dietary treatments: (1) control diet (CON) or (2) VE and alpha-LA supplementation (ANTIOXID). The CON diet was prepared to meet AIN-93M standards, which contains 75 IU VE kg-1 diet. The ANTIOXID diet contained 10 000 IU VE kg(-1) diet and 1.65 g alpha-LA kg(-1) diet. After the 14-week feeding period, significant differences (P<0.05) existed in mean myocardial VE levels between dietary groups. Animals in each experimental group were subjected to an in vivo I-R protocol which included 25 min of left anterior coronary artery occlusion followed by 10 min of reperfusion. No group differences (P>0.05) existed in cardiac performance (e.g. peak arterial pressure or ventricular work) or the incidence of ventricular dysrhythmias during the I-R protocol. Following I-R, two markers of lipid peroxidation were lower (P<0.05) in the ANTIOXID animals compared with CON. These data indicate that dietary supplementation of the antioxidants, VE and alpha-LA do not influence cardiac performance or the incidence of dysrhythmias but do decrease lipid peroxidation during in vivo I-R in young adult rats.
authors.affiliation: Center for Exercise Science, University of Florida, Gainesville, USA.
authors.forename: J S
authors.initials: JS
authors.lastname: Coombes
authors.forename: S K
authors.initials: SK
authors.lastname: Powers
authors.forename: H A
authors.initials: HA
authors.lastname: Demirel
authors.forename: J
authors.initials: J
authors.lastname: Jessup
authors.forename: H K
authors.initials: HK
authors.lastname: Vincent
authors.forename: K L
authors.initials: KL
authors.lastname: Hamilton
authors.forename: H
authors.initials: H
authors.lastname: Naito
authors.forename: R A
authors.initials: RA
authors.lastname: Shanely
authors.forename: C K
authors.initials: CK
authors.lastname: Sen
authors.forename: L
authors.initials: L
authors.lastname: Packer
authors.forename: L L
authors.initials: LL
authors.lastname: Ji
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001555:Benzene Derivatives / Q000378:metabolism; D001794:Blood Pressure / Q000187:drug effects; D019587:Dietary Supplements; D004195:Disease Models, Animal; D005260:Female; D005609:Free Radicals / Q000378:metabolism; D006321:Heart / Q000503:physiopathology; D015227:Lipid Peroxidation / Q000187:drug effects; D009200:Myocardial Contraction / Q000187:drug effects*; D015428:Myocardial Reperfusion Injury / Q000178:diet therapy* / Q000378:metabolism / Q000503:physiopathology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013438:Sulfhydryl Compounds / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D008063:Thioctic Acid / Q000494:pharmacology*; D016276:Ventricular Function / Q000187:drug effects; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D001555:Benzene Derivatives; D005609:Free Radicals; D013438:Sulfhydryl Compounds; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D008063:Thioctic Acid; C007164:cumene hydroperoxide