title: Cells of the yeast Saccharomyces cerevisiae are transformable by DNA under non-artificial conditions.
abstract: Transformants of bakers' yeast (Saccharomyces cerevisiae) can be generated when non-growing cells metabolize sugars (without additional nutrients) in the presence of plasmid DNA. These results suggest that there is a mechanism by which DNA can naturally be taken up by the yeast cell. Natural transformation does not take place in common complete or minimal yeast culture media such as YPD and YNB. The starvation conditions used in our experiments thus seem to be an important prerequisite for such transformation events.
authors.affiliation: Institut für Biotechnologie, Fachgebiet Mikrobiologie und Genetik, Technische Universität Berlin, Seestrasse 13, D-13353 Berlin, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Nevoigt
authors.forename: A
authors.initials: A
authors.lastname: Fassbender
authors.forename: U
authors.initials: U
authors.lastname: Stahl
mesh_terms: D003470:Culture Media; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D013395:Sucrose / Q000378:metabolism*; D014170:Transformation, Genetic
chemical_list: D003470:Culture Media; D013395:Sucrose