title: In vitro cultivation of schizonts of Sarcocystis speeri Dubey and Lindsay, 1999.
abstract: Schizonts of Sarcocystis speeri Dubey and Lindsay, 1999 were cultured in vitro in bovine monocyte and equine kidney cell cultures inoculated with infected tissues of nude and gamma-interferon knockout mice fed sporocysts from opossums, Didelphis albiventris. At least 1 asexual cycle was completed in 3 days. In vitro-grown merozoites were structurally and antigenically distinct from those of Sarcocystis neurona and Sarcocystis falcatula. Culture-derived merozoites of S. speeri were not infective to budgerigars (Melopsittacus undulatus).
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, Livestock and Poultry Sciences Institute, Beltsville, Agricultural Research Center, Maryland 20705-2350, USA.
authors.forename: J P
authors.initials: JP
authors.lastname: Dubey
authors.forename: C A
authors.initials: CA
authors.lastname: Speer
authors.forename: D S
authors.initials: DS
authors.lastname: Lindsay
mesh_terms: D000818:Animals; D001921:Brain / Q000469:parasitology; D002417:Cattle; D002460:Cell Line; D002522:Chlorocebus aethiops; D006651:Histocytochemistry; D006736:Horses; D007150:Immunohistochemistry; D007668:Kidney / Q000166:cytology / Q000469:parasitology; D008099:Liver / Q000469:parasitology; D051379:Mice; D018345:Mice, Knockout; D008819:Mice, Nude; D008858:Microscopy, Phase-Contrast; D009000:Monocytes / Q000166:cytology / Q000469:parasitology; D009893:Opossums / Q000469:parasitology; D010311:Parrots; D012522:Sarcocystis / Q000254:growth & development* / Q000472:pathogenicity; D012523:Sarcocystosis / Q000469:parasitology*