Decrease of neuronal nitric oxide synthase in the cerebellum of aged rats.
Nitric oxide (NO) is produced as an important neurotransmitter in the central nervous system (CNS) to participate in some pathophysiological pathways. In the present study, change of neuronal nitric oxide synthase (nNOS) was examined in isolated cerebellum of Wistar rats aged from 2 to 24 months. Northern blot showed a lower mRNA level of nNOS in rats aged 6, 12 and 24 months than that in rats aged 2 months. Western blot analysis also indicated that the expression of nNOS protein was lower in rats aged 6, 12 and 24 months than that of 2 months rats. However, the activity of nNOS determined by conversion of [(3)H] L-arginine to [(3)H] L-citrulline was decreased significantly in rats aged 24 months only. These results indicate the decrease of NOS expression in cerebellum of aged rat that seems helpful to explain the causes of malfunction in CNS of aged mammalian.
Yu
W
W
Department of Pharmacology, College of Medicine, National Cheng Kung University, Taiwan 70101, Republic of, Tainan City, China.
Juang
S
S
Lee
J
J
Liu
T
T
Cheng
J
J
D000375:Aging / Q000378:metabolism*; D000818:Animals; D001120:Arginine / Q000378:metabolism; D015152:Blotting, Northern; D015153:Blotting, Western; D002531:Cerebellum / Q000201:enzymology*; D002956:Citrulline / Q000096:biosynthesis; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D008297:Male; D019001:Nitric Oxide Synthase / Q000235:genetics / Q000378:metabolism*; D052248:Nitric Oxide Synthase Type I; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D012333:RNA, Messenger; D002956:Citrulline; D001120:Arginine; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; C496322:Nos1 protein, rat; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases