Effect of coumarins on HL-60 cell differentiation.
Twenty-eight coumarins, including 7 furocoumarins, were examined for their activity of induction of terminal differentiation of human promyelocytic leukemia cells (HL-60) by nitro blue tetrazolium (NBT) reducing, nonspecific esterase, specific esterase and phagocytic activities. Esculetin, nordalbergin, 6,7-dihydroxy-4-methylcoumarin and imperatorin had strong activity among the coumarins examined. HL-60 cells treated with these coumarins differentiated into mature monocyte/macrophage. The structure-activity relationship established from the results revealed that 6,7-dihydroxy moiety had an important role in the induction of differentiation of HL-60.
Kawaii
S
S
National Institute of Fruit Tree Science, Shizuoka, Japan.
Tomono
Y
Y
Katase
E
E
Ogawa
K
K
Yano
M
M
D002454:Cell Differentiation / Q000187:drug effects; D003374:Coumarins / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D018922:HL-60 Cells / Q000187:drug effects*; D006801:Humans; D013329:Structure-Activity Relationship
D003374:Coumarins