The Dlk1 and Gtl2 genes are linked and reciprocally imprinted.
Genes subject to genomic imprinting exist in large chromosomal domains, probably reflecting coordinate regulation of the genes within a cluster. Such regulation has been demonstrated for the H19, Igf2, and Ins2 genes that share a bifunctional imprinting control region. We have identified the Dlk1 gene as a new imprinted gene that is paternally expressed. Furthermore, we show that Dlk1 is tightly linked to the maternally expressed Gtl2 gene. Dlk1 and Gtl2 are coexpressed and respond in a reciprocal manner to loss of DNA methylation. These genes are likely to represent a new example of coordinated imprinting of linked genes.
Schmidt
J V
JV
Howard Hughes Medical Institute and Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA.
Matteson
P G
PG
Jones
B K
BK
Guan
X J
XJ
Tilghman
S M
SM
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D019175:DNA Methylation; D017931:DNA Primers; D005260:Female; D018507:Gene Expression Regulation, Developmental; D008040:Genetic Linkage; D018392:Genomic Imprinting; D009115:Muridae / Q000235:genetics; D010542:Peromyscus / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017931:DNA Primers