title: Optimization of the cultivation medium for natamycin production by Streptomyces natalensis.
abstract: The effects of certain nutrients on natamycin production by Streptomyces natalensis in submerged batch culture were studied. The production of this antibiotic required glucose in the cultivation medium with a concentration of 20 g/l. On the other hand, the highest antibiotic production was obtained in a cultivation medium containing 0.05 g/l of potassium dihydrogen phosphate. Further increase in phosphate concentration resulted in a significant increase in biomass concomitant with lower antibiotic production. Among different N-sources tested, only ammonium sulphate, sodium nitrate and beef extract were the suitable nitrogen sources in supporting the antibiotic production. Furthermore, a mixture of beef extract and yeast extract (8 g/l and 2 g/l, respectively) exhibited a synergistic effect in enhancing the natamycin production reaching about 1.5 g/l.
authors.affiliation: Natural and Microbial Products Dept., National Research Centre, Dokki, Cairo, Egypt. nrcfarid@yahoo.com
authors.forename: M A
authors.initials: MA
authors.lastname: Farid
authors.forename: H A
authors.initials: HA
authors.lastname: el-Enshasy
authors.forename: A I
authors.initials: AI
authors.lastname: el-Diwany
authors.forename: A
authors.initials: A
authors.lastname: el-Sayed el-S
mesh_terms: D002244:Carbon / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D005947:Glucose / Q000378:metabolism; D010866:Natamycin / Q000096:biosynthesis*; D009584:Nitrogen / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D013302:Streptomyces / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D003470:Culture Media; D010710:Phosphates; D002244:Carbon; D010866:Natamycin; D005947:Glucose; D009584:Nitrogen