A broad-host-range plasmid for isolating mobile genetic elements in gram-negative bacteria.
Plasmid pGBG1 was constructed to isolate mobile genetic elements in a wide variety of gram-negative bacteria. The mutation target, carried on a broad-host-range vector, allows positive selection for tetracycline resistance. In tests using several gram-negative bacteria we could detect transposition events of either insertion sequences or transposons. A new insertion sequence (IS) element was identified in Ralstonia eutropha.
Schneider
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Plasticité et Expression des Génomes Microbiens, CNRS EP2029, CEA LRC N degrees 12, Université Joseph Fourier, 460 rue de la Piscine, Grenoble Cedex 9, F-38041, France.
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Noirclerc-Savoye
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Barrière
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Coursange
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D016960:Agrobacterium tumefaciens / Q000235:genetics; D016949:Azospirillum / Q000235:genetics; D016956:Burkholderia cepacia / Q000235:genetics; D003227:Conjugation, Genetic; D020118:Cupriavidus necator / Q000235:genetics*; D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D004926:Escherichia coli / Q000235:genetics; D005821:Genetic Techniques; D006090:Gram-Negative Bacteria / Q000235:genetics*; D016297:Mutagenesis, Site-Directed; D010957:Plasmids; D015183:Restriction Mapping; D018626:Retroelements / Q000235:genetics*
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D018626:Retroelements