Immunoblot analysis of trypomastigote excreted-secreted antigens as a tool for the characterization of Trypanosoma cruzi strains and isolates.
An analysis of antibody recognition of Trypanosoma cruzi exoantigens by immunoblotting revealed a unique banding pattern that seems to be characteristic of each strain or isolate. Trypomastigote excreted-secreted antigens (TESA) present in supernatants of LLC-MK2 cells infected with 5 strains and 10 isolates of T. cruzi produced 13 different immunoblotting patterns. The same bands were observed when probed with acute-phase Chagas' disease serum or with serum from a rabbit immunized with the repetitive domain of T. cruzi transialidase recombinant protein (anti-shed acute-phase antigens). Three similar patterns were observed with TESA from 3 human isolates that probably belong to the same T. cruzi strain. When clone CL Brener, clone CL-14, and CL parental strain were analyzed, the same bands were observed, although they presented different biological behavior. These results suggest that immunoblotting analysis of TESA may be a useful tool for characterization of T. cruzi strains and isolates.
Kesper
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N
Instituto de Medicina Tropical de São Paulo, Faculdade de Medicina da Universidade de São Paulo, Laboratório de Protozoologia, Brazil.
de Almeida
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KA
Stolf
A M
AM
Umezawa
E S
ES
D000208:Acute Disease; D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis; D014355:Chagas Disease / Q000276:immunology / Q000469:parasitology*; D002908:Chronic Disease; D006801:Humans; D015151:Immunoblotting; D014349:Trypanosoma cruzi / Q000145:classification* / Q000276:immunology; D014643:Variant Surface Glycoproteins, Trypanosoma
D000953:Antigens, Protozoan; D000954:Antigens, Surface; D014643:Variant Surface Glycoproteins, Trypanosoma; C070842:trypomastigote specific surface antigen