title: Molecular cloning, expression, and antigenicity of Seto virus belonging to genogroup I Norwalk-like viruses.
abstract: The viral capsid protein of the Seto virus (SeV), a Japanese strain of genogroup I Norwalk-like viruses (NLVs), was expressed as virus-like particles using a baculovirus expression system. An antigen detection enzyme-linked immunosorbent assay based on hyperimmune antisera to recombinant SeV was highly specific to homologous SeV-like strains but not heterologous strains in stools, allowing us type-specific detection of NLVs.
authors.affiliation: Laboratory of Virology, Aichi Prefectural Institute of Public Health, Tujimachi, Kita-ku, Nagoya 462-8576, USA. shinkoba@he.mirai.ne.jp
authors.forename: S
authors.initials: S
authors.lastname: Kobayashi
authors.forename: K
authors.initials: K
authors.lastname: Sakae
authors.forename: Y
authors.initials: Y
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Shinozaki
authors.forename: M
authors.initials: M
authors.lastname: Okada
authors.forename: H
authors.initials: H
authors.lastname: Ishiko
authors.forename: K
authors.initials: K
authors.lastname: Kamata
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Natori
authors.forename: T
authors.initials: T
authors.lastname: Miyamura
authors.forename: N
authors.initials: N
authors.lastname: Takeda
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D015139:Blotting, Southern; D002139:Caliciviridae / Q000145:classification* / Q000235:genetics / Q000276:immunology*; D017250:Caliciviridae Infections / Q000821:virology*; D002213:Capsid / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D009663:Norwalk virus / Q000145:classification / Q000235:genetics / Q000276:immunology; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000956:Antigens, Viral; D011994:Recombinant Proteins