title: Detection of spatial localization of Hst-1/Fgf-4 gene expression in brain and testis from adult mice.
abstract: HST-1, a member of the fibroblast growth factor (FGF) family (FGF-4), has been shown to be a signaling molecule whose expression is essential for embryonic development. However, HST-1/FGF-4 expression has not been detected or reported in adult tissues so far analysed. To investigate whether there is a possible role of HST-1/FGF-4 in adult stage, we have carried out a highly sensitive RT-PCR analysis of Hst-1/Fgf-4 gene expression in adult mice tissues. Results show Hst-1/Fgf-4 gene expression in the nervous system, intestines, and testis of normal adult mice. In situ hybridization technique was used to localize Hst-1/Fgf-4 gene expression in the cerebellum and testis from 10-week-old mice. Cell type-specific gene expression was detected: Purkinje cells in the cerebellum and Sertoli cells in testis. These findings suggest that the Hst-1/Fgf-4 gene also plays an important role in adult tissues, and may offer insights into the biological significance of HST-1/FGF-4 in cerebellar and testicular functions.
authors.affiliation: National Cancer Center Research Institute, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yamamoto
authors.forename: T
authors.initials: T
authors.lastname: Ochiya
authors.forename: Y
authors.initials: Y
authors.lastname: Takahama
authors.forename: Y
authors.initials: Y
authors.lastname: Ishii
authors.forename: N
authors.initials: N
authors.lastname: Osumi
authors.forename: H
authors.initials: H
authors.lastname: Sakamoto
authors.forename: M
authors.initials: M
authors.lastname: Terada
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001921:Brain / Q000196:embryology / Q000378:metabolism*; D002478:Cells, Cultured; D005260:Female; D051519:Fibroblast Growth Factor 4; D005346:Fibroblast Growth Factors / Q000235:genetics*; D015870:Gene Expression; D017403:In Situ Hybridization / Q000379:methods; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D012333:RNA, Messenger; D013737:Testis / Q000196:embryology / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: C496446:Fgf4 protein, mouse; D051519:Fibroblast Growth Factor 4; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D005346:Fibroblast Growth Factors