Investigation of the susceptibility of equine autonomic neuronal cell lines, clonally derived from the same paravertebral ganglion, to toxic plasma from equine dysautonomia (grass sickness) cases.
In the autonomic nervous system (ANS) of equine grass sickness (GS) cases, some neurones show abnormal changes while neighbouring neurones are unaffected. To test whether noradrenergic neurones showed variable susceptibility to the GS toxin in culture, clonally-derived populations isolated from the same fetal thoracic sympathetic chain ganglion were challenged with plasma from GS cases previously shown to induce ANS damage when injected into normal horses. During the early stages of exposure to toxic plasma, cells within a clonal population showed variable susceptibility ranging from no obvious effect to characteristic patterns of pathology. However, after 3 days of exposure to toxic plasma all cells were killed. Dose response analysis on selected clonal populations showed no significant difference in TD(50) values.
John
H A
HA
Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian EH26 0PZ, Scotland, UK. Johnh@mri.sari.ac.uk
Marrs
J
J
Laffling
A J
AJ
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000378:metabolism; D001341:Autonomic Nervous System / Q000378:metabolism / Q000473:pathology*; D001342:Autonomic Nervous System Diseases / Q000097:blood / Q000662:veterinary*; D002460:Cell Line; D002999:Clone Cells; D004299:Dopamine beta-Hydroxylase / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D005728:Ganglia, Sympathetic / Q000378:metabolism / Q000473:pathology*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006734:Horse Diseases / Q000097:blood*; D006736:Horses; D009474:Neurons / Q000378:metabolism / Q000473:pathology*; D009478:Neuropeptide Y / Q000378:metabolism; D010949:Plasma / Q000502:physiology*; D013909:Thorax / Q000294:innervation
D000952:Antigens, Polyomavirus Transforming; D005904:Glial Fibrillary Acidic Protein; D009478:Neuropeptide Y; D004299:Dopamine beta-Hydroxylase