Production and characterization of antibodies directed against the human melatonin receptors Mel-1a (mt1) and Mel-1b (MT2).
We report the production of polyclonal antibodies directed against the human melatonin receptors Mel-1a (mt1) and Mel-1b (MT2) by means of antigenic synthetic peptides with sequences unique to these proteins. Immunostaining on NIH3T3 cells stably transfected with Mel-1a and Mel-1b cDNA gave intense reactions. Neither the preimmune serum nor cross-tested antisera showed any reactivity. These polyclonal antibodies will be essential immunocytochemical tools to study the human melatonin receptors distribution at subcellular level.
Angeloni
D
D
Department of Pharmacology, University of Milan, Italy. andreazzolid@mail.ncifcrf.gov
Longhi
R
R
Fraschini
F
F
D016475:3T3 Cells; D000818:Animals; D000917:Antibody Formation; D006801:Humans; D051379:Mice; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D018160:Receptors, Cytoplasmic and Nuclear / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D044094:Receptors, Melatonin
D011956:Receptors, Cell Surface; D018160:Receptors, Cytoplasmic and Nuclear; D044094:Receptors, Melatonin