Anti-HSP70 autoantibody formation by B-1 cells in Toxoplasma gondii-infected mice.
Formation of anti-Toxoplasma gondii HSP70 (TgHSP70) antibody cross-reactive to mouse HSP70 (mHSP70) was observed in the sera of BALB/c (a resistant strain) and C57BL/6 (B6; a susceptible strain) mice after peroral infection with T. gondii cysts of the Fukaya strain. The levels of anti-mHSP70 immunoglobulin G (IgG) autoantibody production in B6 mice were higher than those in BALB/c mice. The isotype and subclass of IgG of anti-TgHSP70 monoclonal antibodies cross-reactive to mHSP70 were mu and gamma3. Anti-mHSP70 autoantibody in T. gondii-infected BALB/c and B6 mice was shown to be produced by the CD5(+) subset of B cells (B-1a cells) but not by conventional B cells (B-2 cells). The epitopes recognized by anti-mHSP70 autoantibody were located primarily in the C-terminal fragment of mHSP70.
Chen
M
M
Department of Parasitology, Chiba University School of Medicine, Chuo-ku, Chiba 260-8670, Japan.
Aosai
F
F
Mun
H S
HS
Norose
K
K
Hata
H
H
Yano
A
A
D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis* / Q000276:immunology; D001323:Autoantibodies / Q000096:biosynthesis* / Q000276:immunology; D003429:Cross Reactions; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D014124:Toxoplasmosis, Animal / Q000276:immunology*
D000913:Antibodies, Protozoan; D001323:Autoantibodies; D000939:Epitopes; D018840:HSP70 Heat-Shock Proteins