Monosaccharide compositions of Danaus plexippus (monarch butterfly) and Trichoplusia ni (cabbage looper) egg glycoproteins.
Monosaccharide compositions of eggs from Danaus plexippus (monarch butterfly) and Trichoplusia ni (cabbage looper) were analyzed. Analyses were performed mainly with high performance anion exchange chromatography (HPAEC) using crude extracts of eggs or SDS-PAGE separated and PVDF-blotted protein bands. Man and GlcN were the major components in all cases, but low levels of Gal and Fuc were possibly present in some samples. Some T. ni egg glycoproteins even contained GalN. Although a peak comigrating with Neu5Ac could be detected with HPAEC-PAD or RP-HPLC (fluorometry) after derivatization with 1,2-diamino-4,5-methylenedioxy-benzene, the quantities were too small to be significant as an integral part of the analyzed glycoproteins. These data suggests that most of glycans on the glycoproteins are pauci-Man type N-glycans, but a small portion of N-glycan may be either hybrid type or complex type.
Park
Y I
YI
Biology Department, Johns Hopkins University, Baltimore, Maryland 21218, USA.
Wood
H A
HA
Lee
Y C
YC
D000117:Acetylglucosamine / Q000032:analysis; D000818:Animals; D002457:Cell Extracts; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000737:chemistry*; D006031:Glycosylation; D019476:Insect Proteins / Q000737:chemistry*; D007915:Lepidoptera / Q000737:chemistry* / Q000166:cytology; D008358:Mannose / Q000032:analysis; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis*; D010063:Ovum / Q000737:chemistry* / Q000166:cytology; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry; D012794:Sialic Acids / Q000032:analysis
D002457:Cell Extracts; D006023:Glycoproteins; D019476:Insect Proteins; D009005:Monosaccharides; D011134:Polysaccharides; D012794:Sialic Acids; D008358:Mannose; D000117:Acetylglucosamine