title: Maurotoxin versus Pi1/HsTx1 scorpion toxins. Toward new insights in the understanding of their distinct disulfide bridge patterns.
abstract: Maurotoxin (MTX) is a scorpion toxin acting on several K(+) channel subtypes. It is a 34-residue peptide cross-linked by four disulfide bridges that are in an "uncommon" arrangement of the type C1-C5, C2-C6, C3-C4, and C7-C8 (versus C1-C5, C2-C6, C3-C7, and C4-C8 for Pi1 or HsTx1, two MTX-related scorpion toxins). We report here that a single mutation in MTX, in either position 15 or 33, resulted in a shift from the MTX toward the Pi1/HsTx1 disulfide bridge pattern. This shift is accompanied by structural and pharmacological changes of the peptide without altering the general alpha/beta scaffold of scorpion toxins.
authors.affiliation: CNRS UMR 6560, Boulevard Pierre Dramard, 13916 Marseille Cedex 20, the Architecture et Fonction des Macromolécules Biologiques, CNRS UPR 9039, 31 Chemin Joseph Aiguier, 13402 Marseille, France.
authors.forename: Z
authors.initials: Z
authors.lastname: Fajloun
authors.forename: A
authors.initials: A
authors.lastname: Mosbah
authors.forename: E
authors.initials: E
authors.lastname: Carlier
authors.forename: P
authors.initials: P
authors.lastname: Mansuelle
authors.forename: G
authors.initials: G
authors.lastname: Sandoz
authors.forename: M
authors.initials: M
authors.lastname: Fathallah
authors.forename: E
authors.initials: E
authors.lastname: di Luccio
authors.forename: C
authors.initials: C
authors.lastname: Devaux
authors.forename: H
authors.initials: H
authors.lastname: Rochat
authors.forename: H
authors.initials: H
authors.lastname: Darbon
authors.forename: M
authors.initials: M
authors.lastname: De Waard
authors.forename: J M
authors.initials: JM
authors.lastname: Sabatier
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000737:chemistry; D004220:Disulfides; D004305:Dose-Response Relationship, Drug; D004594:Electrophysiology; D007700:Kinetics; D007928:Lethal Dose 50; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008810:Mice, Inbred C57BL; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D009865:Oocytes / Q000378:metabolism; D010452:Peptide Biosynthesis; D017354:Point Mutation; D015221:Potassium Channels / Q000737:chemistry; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D051381:Rats; D012604:Scorpion Venoms / Q000737:chemistry* / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013574:Synaptosomes / Q000378:metabolism; D013997:Time Factors; D014981:Xenopus
chemical_list: D004220:Disulfides; D008024:Ligands; C104751:Pi1 toxin; D015221:Potassium Channels; D012604:Scorpion Venoms; C109471:TX1 toxin, Heterometrus spinnifer; C104498:maurotoxin; D003545:Cysteine