An NMR spectroscopic identity test for the control of the capsular polysaccharide from Haemophilus influenzae type b.
We describe the use of Nuclear Magnetic Resonance (NMR) spectroscopy to control the identity of purified bulk capsular polysaccharide [called poly(ribosylribitolphosphate) or PRP] from Haemophilus influenzae type b (Hib), and derivatised forms, used in the production of Hib polysaccharide-protein conjugate vaccines. We describe the approaches we have developed to validate this test.
Lemercinier
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Laboratory for Molecular Structure, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Herts, EN6 3QG, UK.
Jones
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C
D018073:Haemophilus Vaccines / Q000737:chemistry / Q000592:standards*; D006193:Haemophilus influenzae / Q000145:classification / Q000276:immunology*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D011135:Polysaccharides, Bacterial / Q000276:immunology* / Q000302:isolation & purification*; D011786:Quality Control; D012680:Sensitivity and Specificity; D018074:Vaccines, Conjugate / Q000737:chemistry / Q000592:standards*
D018073:Haemophilus Vaccines; D011135:Polysaccharides, Bacterial; D018074:Vaccines, Conjugate