Immune system activation by CDDP and "poly-plat".
Murine peritoneal macrophages, human fibroblasts and human ovarian teratocarcinoma cells were treated with CDDP (10 micrograms/ml) and "poly-plat" (10 micrograms/ml) for 2 h and cultured for 2-96 h. After "poly-plat" treatment macrophages developed cytoplasmic extensions much faster and secreted higher levels of interleukin-2 (IL-2), compared to CDDP. "Poly-plat" treated human fibroblasts demonstrated a significant release of IL-2 (138 pg/ml), compared to CDDP (64 pg/ml) or the untreated normal cells (31 pg/ml) after 8 h. Correspondingly there was a 53% increase in cells after "poly-plat" treatment compared to only 37% after CDDP. However, human ovarian teratocarcinoma cells didn't show any significant increase in IL-2 levels after "poly-plat" treatment and there was instead a decrease of 15% in cell numbers at 8 h. Based on our studies, we propose that "poly-plat" is more effective in activation of the macrophages and human fibroblasts in terms of IL-2, but not so with human ovarian teratocarcinoma cells.
Burkitt
K
K
Department of Zoology, Michigan State University, East Lansing 48824-1115, USA.
Aggarwal
S K
SK
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002945:Cisplatin / Q000494:pharmacology*; D005260:Female; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism; D008262:Macrophage Activation / Q000187:drug effects*; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000473:pathology; D051379:Mice; D009944:Organoplatinum Compounds / Q000494:pharmacology*; D010051:Ovarian Neoplasms / Q000473:pathology; D018243:Teratocarcinoma / Q000473:pathology
D000276:Adjuvants, Immunologic; D000970:Antineoplastic Agents; D007376:Interleukin-2; D009944:Organoplatinum Compounds; C111406:poly(trans-1,2-diaminocyclohexane)platinum-carboxyamylose; D002945:Cisplatin