Crystallization and preliminary crystallographic studies of bar-headed goose fluoromethaemoglobin with inositol hexaphosphate.
Bar-headed goose fluoromethaemoglobin (fluoromet-Hb) complexed with inositol hexaphosphate (IHP) has been crystallized using PEG 6000 as precipitant. The crystal belongs to space group P2(1), with unit-cell parameters a = 59.8, b = 72.0, c = 79.8 A, beta = 102.1 degrees, and diffracts to 2.5 A resolution. To prove the presence of IHP, the structure was determined by the molecular-replacement method. IHP was observed at the entrance to the central cavity between the N and C termini of two beta subunits.
Wang
H C
HC
The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, People's Republic of China.
Liang
Y H
YH
Zhu
J P
JP
Lu
G Y
GY
D000494:Allosteric Regulation; D000818:Animals; D001665:Binding Sites; D003460:Crystallization; D018360:Crystallography, X-Ray; D005777:Geese; D006454:Hemoglobins / Q000378:metabolism; D008706:Methemoglobin / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000378:metabolism; D010100:Oxygen / Q000097:blood; D010833:Phytic Acid / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding
D006454:Hemoglobins; C030944:fluoro-methemoglobin; D010833:Phytic Acid; D008706:Methemoglobin; D010100:Oxygen