Pathophysiological role of T lymphocytes in renal ischemia-reperfusion injury in mice.
Mononuclear cell infiltrates are found in human renal ischemia-reperfusion injury (IRI), and peritubular T lymphocytes have been identified in experimental IRI. However, the role of T cells in the pathogenesis of renal IRI is unknown. We hypothesized that T cells are one of the important mediators of renal IRI. To test this hypothesis, we used an established mouse model of renal IRI, and evaluated mice with genetically engineered deficiency of both CD4+ and CD8+ T cells. At 48 h postischemia, CD4/CD8-knockout (KO) mice had marked improvement in renal function compared with control C57BL/6 mice (serum creatinine: 0.7 +/- 0.4 vs. 2.5 +/- 0.3 mg/dl, respectively; P < 0.05). Neutrophil infiltration into postischemic kidney was reduced in CD4/CD8 KO mice, compared with control mice, at both 24 h [polymorphonuclear neutrophils (PMNs)/10 high power fields: 714 +/- 354 vs. 3,514 +/- 660, respectively; P < 0.05] and 48 h (88 +/- 32 vs. 1,979 +/- 209, respectively; P < 0.05). Tubular necrosis score in CD4/CD8 KO mice, compared with control mice, was significantly less at 48 h (0.4 +/- 0.1 vs. 2.4 +/- 0.2, respectively; P < 0.05). Because adhesion between T cells and renal tubular epithelial cells (RTECs) may underlie the pathophysiological role of T cells in renal IRI, we also measured T cell adhesion to primary murine RTECs in vitro. Exposure of RTECs to 2 h of hypoxia followed by 1 h of reoxygenation increased T cell adhesion more than twofold. Phorbol ester treatment, which activates integrins, increased T cell adhesion threefold. These data suggest that T lymphocytes can mediate experimental renal IRI. Moreover, adhesion of infiltrating T cells to renal tubular cells may provide a potential mechanism underlying postischemic tubular dysfunction.
Rabb
H
H
Department of Internal Medicine, Hennepin County Medical Center, University of Minnesota Medical School, Minneapolis, Minnesota 55415, USA. rabbx003@tc.umn.edu
Daniels
F
F
O'Donnell
M
M
Haq
M
M
Saba
S R
SR
Keane
W
W
Tang
W W
WW
D058186:Acute Kidney Injury / Q000276:immunology* / Q000503:physiopathology*; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002448:Cell Adhesion / Q000276:immunology; D002465:Cell Movement / Q000276:immunology; D015194:Diffusion Chambers, Culture / Q000295:instrumentation / Q000379:methods; D004195:Disease Models, Animal; D000860:Hypoxia / Q000276:immunology / Q000503:physiopathology; D007668:Kidney / Q000098:blood supply / Q000502:physiology; D007683:Kidney Tubular Necrosis, Acute / Q000276:immunology / Q000503:physiopathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009393:Nephritis / Q000276:immunology / Q000503:physiopathology; D009504:Neutrophils / Q000166:cytology / Q000276:immunology; D015427:Reperfusion Injury / Q000276:immunology* / Q000503:physiopathology*