title: Phase I pharmacokinetic trial of perillyl alcohol (NSC 641066) in patients with refractory solid malignancies.
abstract: Perillyl alcohol (POH) is a monoterpene with anticarcinogenic and antitumor activity in murine tumor models. Putative mechanisms of action include activation of the transforming growth factor beta pathway and/or inhibition of p21ras signaling, leading to differentiation or apoptosis. In this Phase I trial, 17 patients took POH p.o. three times daily for 14 days of each 28-day cycle. The starting dose of POH was 1600 mg/m2/dose, with escalations to 2100 and 2800 mg/m2/dose in subsequent cohorts. Chronic nausea and fatigue were dose-limiting toxic effects at 2800 mg/m2. Grade 1-2 hypokalemia was common at 2100 and 2800 mg/m2. Although POH could not be detected in plasma, two of its metabolites, dihydroperillic acid (DHPA) and perillic acid (PA), were measured in plasma and urine on days 1 and 15 after the first and last doses of POH, respectively. Both area under the concentration versus time curve and peak plasma concentration (Cmax) values increased with dose and exhibited high intersubject variability. Day 15 DHPA Cmax values ranged from a mean +/- SD of 22.6+/-12 microM at 1600 mg/m2/dose to 42.4+/-15.24 microM at 2800 mg/m2/dose. Corresponding mean +/- SD Cmax values for PA were 433.2+/-245.8 and 774.1+/-439.6 microM. One patient treated at the 2800 mg/m2/dose had markedly prolonged plasma levels of both PA and DHPA and developed grade 3 mucositis. POH treatment did not consistently alter the expression of p21ras, rap1, or rhoA in peripheral blood mononuclear cells obtained from patients treated at the highest dose level. The metabolites PA and DHPA did not change expression or isoprenylation of p21ras in MCF-7 breast or DU145 prostate carcinoma cells at concentrations that exceeded those achieved in patient plasma after POH treatment. We conclude that POH at 1600-2100 mg/m2 p.o. three times daily is well tolerated on a 14-day on/14-day off dosing schedule. Inhibition of p21ras function in humans is not likely to occur after POH administration at safe doses of the present oral formulation.
authors.affiliation: Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA. g_hudes@fccc.edu
authors.forename: G R
authors.initials: GR
authors.lastname: Hudes
authors.forename: C E
authors.initials: CE
authors.lastname: Szarka
authors.forename: A
authors.initials: A
authors.lastname: Adams
authors.forename: S
authors.initials: S
authors.lastname: Ranganathan
authors.forename: R A
authors.initials: RA
authors.lastname: McCauley
authors.forename: L M
authors.initials: LM
authors.lastname: Weiner
authors.forename: C J
authors.initials: CJ
authors.lastname: Langer
authors.forename: S
authors.initials: S
authors.lastname: Litwin
authors.forename: G
authors.initials: G
authors.lastname: Yeslow
authors.forename: T
authors.initials: T
authors.lastname: Halberr
authors.forename: M
authors.initials: M
authors.lastname: Qian
authors.forename: J M
authors.initials: JM
authors.lastname: Gallo
mesh_terms: D000284:Administration, Oral; D000328:Adult; D000368:Aged; D000972:Antineoplastic Agents, Phytogenic / Q000009:adverse effects* / Q000493:pharmacokinetics* / Q000627:therapeutic use; D053138:Cyclohexenes; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D039821:Monoterpenes; D009369:Neoplasms / Q000097:blood / Q000188:drug therapy / Q000378:metabolism*; D016283:Proto-Oncogene Proteins p21(ras) / Q000096:biosynthesis / Q000097:blood; D013729:Terpenes / Q000009:adverse effects* / Q000097:blood / Q000493:pharmacokinetics* / Q000627:therapeutic use / Q000652:urine; D014407:Tumor Cells, Cultured
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D053138:Cyclohexenes; D039821:Monoterpenes; D013729:Terpenes; C032208:perillyl alcohol; C078707:dihydroperillic acid; C078706:perillic acid; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)