title: New aqueous two-phase system based on cashew-nut tree gum and poly(ethylene glycol).
abstract: The characterisation of a new system based on cashew-nut tree gum, a branched acidic heteropolysaccharide found in Brazil, and poly(ethylene glycol) (PEG) was studied. Phase diagrams are provided for the PEG-cashew-nut tree gum system. The influence of PEG molecular mass, tie-line length and pH on bovine serum albumin (BSA) partition was investigated. Protein partition coefficient was little influenced by changing PEG molecular mass. Increasing the tie-line length decreased the partition. Increasing the pH also raised the BSA partition coefficient. It is shown that systems formed by PEG and cashew-nut tree gum may be considered as an interesting alternative for use in protein purification.
authors.affiliation: DESQ-FEQ, UNICAMP, Campinas-SP-Brasil, Brazil.
authors.forename: L A
authors.initials: LA
authors.lastname: Sarubbo
authors.forename: L A
authors.initials: LA
authors.lastname: Oliveira
authors.forename: A L
authors.initials: AL
authors.lastname: Porto
authors.forename: H S
authors.initials: HS
authors.lastname: Duarte
authors.forename: A M
authors.initials: AM
authors.lastname: Carneiro-Leão
authors.forename: J L
authors.initials: JL
authors.lastname: Lima-Filho
authors.forename: G M
authors.initials: GM
authors.lastname: Campos-Takaki
authors.forename: E B
authors.initials: EB
authors.lastname: Tambourgi
mesh_terms: D002240:Carbohydrate Sequence; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011092:Polyethylene Glycols / Q000737:chemistry*; D012116:Resins, Plant / Q000737:chemistry*; D014197:Trees / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D012116:Resins, Plant; D014867:Water; D011092:Polyethylene Glycols