title: Cytosolic [Ca(2+)] modulates basal GLUT1 activity and plays a permissive role in its activation by metabolic stress and insulin in rat epithelial cells.
abstract: The aim of this work was to investigate the role of cytosolic free calcium ([Ca(2+)]c) in the stimulation of GLUT1 by metabolic stress and insulin. Chelation of [Ca(2+)]c with bapta, introduced in rat liver epithelial Clone 9 cells in the acetoxymethyl (AM) form, decreased their basal rate of 2-deoxyglucose uptake in a dose-dependent fashion. Maximal inhibition at 75 microM bapta was by 38 +/- 8% (n = 8). The effect was partially reversed by ionomycin. Basal sugar uptake was also decreased by lowering extracellular [Ca(2+)] in ionomycin-permeabilized cells. Increasing [Ca(2+)]c over its resting level of 168 +/- 32 (n = 27) had no affect on sugar uptake. Chelation of [Ca(2+)]c did not change the abundance of surface GLUT1 and had no significant effect on the affinity of GLUT1 for sugars. In addition, calcium chelation abolished the activation of GLUT1 by azide, arsenate, 2,4-dinitrophenol and insulin. However, [Ca(2+)]c did not increase in the presence of azide. We conclude that [Ca(2+)]c, near or below its resting level, modulates GLUT1 activity over a considerable range and plays a permissive role in the activation of the carrier by metabolic stress and insulin.
authors.affiliation: Centro de Estudios Científicos (CECS), Valdivia, Chile.
authors.forename: R A
authors.initials: RA
authors.lastname: Quintanilla
authors.forename: O H
authors.initials: OH
authors.lastname: Porras
authors.forename: J
authors.initials: J
authors.lastname: Castro
authors.forename: L F
authors.initials: LF
authors.lastname: Barros
mesh_terms: D019297:2,4-Dinitrophenol / Q000494:pharmacology; D000818:Animals; D001149:Arsenates / Q000494:pharmacology; D001386:Azides / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002463:Cell Membrane Permeability / Q000187:drug effects; D002478:Cells, Cultured; D002614:Chelating Agents / Q000494:pharmacology; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism*; D003847:Deoxyglucose / Q000493:pharmacokinetics; D004305:Dose-Response Relationship, Drug; D004533:Egtazic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D051272:Glucose Transporter Type 1; D007328:Insulin / Q000378:metabolism* / Q000494:pharmacology; D015759:Ionomycin / Q000494:pharmacology; D007476:Ionophores / Q000494:pharmacology; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D051381:Rats
chemical_list: D001149:Arsenates; D001386:Azides; D002614:Chelating Agents; D051272:Glucose Transporter Type 1; D007328:Insulin; D007476:Ionophores; D009004:Monosaccharide Transport Proteins; C495679:Slc2a1 protein, rat; D004533:Egtazic Acid; D015759:Ionomycin; D003847:Deoxyglucose; C025603:1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid; C025657:arsenic acid; D019297:2,4-Dinitrophenol; D002118:Calcium