title: DNA ploidy analysis and cell proliferation in congenital sacrococcygeal teratomas.
abstract: BACKGROUND
  Congenital sacrococcygeal teratoma is the most common germ cell tumor in infants and children. It usually is diagnosed at birth, is benign, and consists of fully differentiated mature tissues. Congenital sacrococcygeal teratomas (SCTs) also may contain immature tissues, most commonly of neural origin. The proportion of malignant teratomas increases with advancing age, but the relation between mature and immature SCTs is not well understood. Thus, it is very important to determine proliferative activity, DNA ploidy, and DNA index to predict biologic behavior of these tumors.
  
  
  METHODS
  DNA ploidy and cell proliferation were analyzed by flow cytometry, and the expression of proliferating cell nuclear antigen (PCNA) and Ki-67 were analyzed immunohistochemically on paraffin embedded tissue.
  
  
  RESULTS
  All the tumors that were surgically treated within 3 months after birth, including immature teratoma, were diploid. Strongly positive PCNA immunostaining was found in both immature teratomas, and weakly positive PCNA was found in nine cases. Weak positivity for Ki-67 was observed in 2 cases, and moderate positivity was observed in 6 cases including immature teratomas.
  
  
  CONCLUSIONS
  The value of flow cytometry in the prediction of biologic behavior of congenital SCT should be analyzed further. Our results suggest that Ki-67 and especially PCNA may reflect the proliferative activity of these tumors.
authors.affiliation: Department of Pathology, School of Medicine, University of Zagreb, Zagreb, Croatia.
authors.forename: B
authors.initials: B
authors.lastname: Kruslin
authors.forename: A
authors.initials: A
authors.lastname: Visnjić
authors.forename: A
authors.initials: A
authors.lastname: Cizmic
authors.forename: I
authors.initials: I
authors.lastname: Tomicić
authors.forename: M
authors.initials: M
authors.lastname: Kos
authors.forename: S
authors.initials: S
authors.lastname: Jukić
authors.forename: S
authors.initials: S
authors.lastname: Seiwerth
mesh_terms: D002455:Cell Division / Q000235:genetics; D002675:Child, Preschool; D004247:DNA / Q000032:analysis; D004273:DNA, Neoplasm; D004171:Diploidy; D005260:Female; D005434:Flow Cytometry; D018237:Germinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D019394:Ki-67 Antigen / Q000032:analysis; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D012445:Sacrococcygeal Region / Q000473:pathology; D013724:Teratoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: D004273:DNA, Neoplasm; D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen; D004247:DNA