Determination of defensin HNP-1, HNP-2, and HNP-3 in human saliva by using LC/MS.
The mass spectral profiling of saliva by liquid chromatography mass spectrometry in relation to particular types of pain is being examined. The aim is to develop a profile that could be useful for the assessment of patients and their treatment programs, as well as identifying unknown compounds observed in saliva. Defensin human neutrophil peptide-1 (HNP-1) and defensin HNP-2 were identified and confirmed, whereas defensin HNP-3 was tentatively identified. Linear calibration range of defensin HNP-1 and HNP-2 was 0.25 to 3 microg/ml with R(2) values of > 0.99 for both. The detection limit for defensin HNP-1 and HNP-2 was estimated at 0.1 microg/ml. The healthy subjects surveyed in this study had readily measurable salivary concentrations of defensin HNP-1 (8.6 +/- SD 8.0 microg/ml) and defensin HNP-2 (5.6 +/- SD 5.2 microg/ml).
Goebel
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Research and Development Section, Australian Government Analytical Laboratories, 1 Suakin St., 2073, Pymble, New South Wales, Australia. catrin.goebel@agal.gov.au
Mackay
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Vickers
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Mather
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LE
D000328:Adult; D000890:Anti-Infective Agents / Q000032:analysis*; D023082:Defensins; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011506:Proteins / Q000032:analysis*; D012463:Saliva / Q000737:chemistry*; D023084:alpha-Defensins
D000890:Anti-Infective Agents; D023082:Defensins; D011506:Proteins; D023084:alpha-Defensins; C406413:human neutrophil peptide 1; C406416:human neutrophil peptide 2; C406427:human neutrophil peptide 3