title: Measuring conformational dynamics of biomolecules by single molecule fluorescence spectroscopy.
abstract: Dynamic structural changes of macromolecules undergoing biochemical reactions can be studied using novel single molecule spectroscopy tools. Recent advances in applying such distance and orientation molecular rulers to biological systems are reviewed, and future prospects and challenges are discussed.
authors.affiliation: Materials Sciences and Physical Biosciences Divisions, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA. sweiss@lbl.gov
authors.forename: S
authors.initials: S
authors.lastname: Weiss
mesh_terms: D000818:Animals; D002477:Cells / Q000737:chemistry / Q000378:metabolism; D004735:Energy Transfer; D006801:Humans; D008968:Molecular Conformation; D017510:Protein Folding; D006180:Proton-Translocating ATPases / Q000737:chemistry / Q000378:metabolism; D016337:RNA, Catalytic / Q000737:chemistry / Q000378:metabolism; D015203:Reproducibility of Results; D012399:Rotation; D013050:Spectrometry, Fluorescence / Q000379:methods* / Q000639:trends; D013816:Thermodynamics
chemical_list: D016337:RNA, Catalytic; D006180:Proton-Translocating ATPases