Rapid analysis of amplified double-stranded DNA by capillary electrophoresis with laser-induced fluorescence detection.
DNA amplification technology has been applied to clinical diagnosis of infectious disease, genetic disorder, and cancer. After in vitro amplification of a particular DNA region, the methods of analysis for these amplified samples play a pivotal role in clinical diagnosis. Conventional gel electrophoresis has been routinely used in the lab for checking DNA. The whole procedure is time consuming and requires more than 1 ng of DNA for detection. To achieve greater performance in DNA diagnosis, we demonstrated capillary electrophoresis with laser induced fluorescence detection for analysis of amplified DNA. The analysis of DNA could be completed within 3 min and the data is directly entered into the computer. Considering the automatic and rapid process, we believe that this method could be routinely utilized for the clinical diagnosis of amplified DNA products.
Liu
M S
MS
Beckman Coulter Inc., Fullerton, CA 92834, USA.
Chen
F T
FT
D004247:DNA / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000706:statistics & numerical data; D005453:Fluorescence; D005784:Gene Amplification; D006801:Humans; D007834:Lasers; D016133:Polymerase Chain Reaction / Q000379:methods
D004247:DNA