Global analysis of gene expression in cells of the immune system II. Cell-free translation products and high-density filter hybridization data.
We have developed an experimental system for linking information on cell-free transcription and translation products from cDNA clones with data obtained from hybridization signals from complex probes. The work described in this paper consists of two distinct processes, one being the construction of a system of clonal addresses and the other the identification of expressed genes involved in the studied processes. We describe the use of this system to identify genes involved in thymus development. Complex probes from fetal thymuses (GD15, 17 and newborn) of Balb/c mice were used to identify genes, which are up- or downregulated during the process of differentiation. The full set of information is available in the Clone-base of the Basel Institute for Immunology and will be retrievable from the website of the collaborating laboratories.
Frey
J R
JR
Basel Institute for Immunology, Switzerland.
Nguyen
C
C
Houlgatte
R
R
Jordan
B
B
Auffray
C
C
Fol
B
B
Kuhn
L
L
Meyer
S
S
Hartmann
K U
KU
Coleclough
C
C
Kettman
J R
JR
Lefkovits
I
I
D000818:Animals; D000831:Animals, Newborn; D001402:B-Lymphocytes / Q000737:chemistry; D001483:Base Sequence; D002474:Cell-Free System; D018076:DNA, Complementary / Q000235:genetics*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D004926:Escherichia coli; D005374:Filtration; D020869:Gene Expression Profiling / Q000379:methods*; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D007091:Image Processing, Computer-Assisted; D008163:Luminescent Measurements; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization / Q000379:methods*; D014176:Protein Biosynthesis; D020543:Proteome; D012333:RNA, Messenger / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000032:analysis*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D013950:Thymus Gland / Q000737:chemistry / Q000166:cytology* / Q000196:embryology / Q000254:growth & development
D018076:DNA, Complementary; D020543:Proteome; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins