Metabolic effects induced by epinephrine in Rana balcanica erythrocytes under normoxic and hypoxic conditions.
The metabolic effects of epinephrine on Rana balacanica erythrocyte suspension were studied under normoxia and hypoxia. After epinephrine treatment, a 1.2-fold increase of lactate formation and a 20 per cent decrease of ATP concentration was found under normoxic conditions. These effects were rapid and specific to beta, alpha(1) and alpha(2) antagonists. Glycolysis was stimulated to almost the same extent by both epinephrine and forskolin as normoxic conditions. The stimulation of glycolysis was probably due to stimulation of phosphofructokinase (PFK) as well as to activation of Na(+), K(+)-ATPase. The decrease of ATP was a contributing factor to PFK activation. Despite the high levels of c-AMP at hypoxia, glycolysis was not further induced by epinephrine.
Kaloyianni
M
M
Laboratory of Animal Physiology, Department of Zoology, Science School, Aristotle University of Thessaloniki, Thessaloniki 54006, Greece.
Papaefthimiou
I
I
Simeonidou
V
V
D000255:Adenosine Triphosphate / Q000097:blood; D000322:Adrenergic Agonists / Q000494:pharmacology*; D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D004837:Epinephrine / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects*; D005947:Glucose / Q000378:metabolism; D000860:Hypoxia; D019344:Lactic Acid / Q000097:blood; D010042:Ouabain / Q000494:pharmacology; D010100:Oxygen / Q000378:metabolism*; D010472:Perchlorates / Q000494:pharmacology; D010732:Phosphofructokinase-1 / Q000378:metabolism; D011224:Prazosin / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D019289:Pyruvic Acid / Q000097:blood; D011898:Ranidae / Q000097:blood*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013997:Time Factors; D015016:Yohimbine / Q000494:pharmacology
D000322:Adrenergic Agonists; D000317:Adrenergic alpha-Antagonists; D000319:Adrenergic beta-Antagonists; D010472:Perchlorates; D005576:Colforsin; D015016:Yohimbine; D019344:Lactic Acid; D010042:Ouabain; D019289:Pyruvic Acid; D000255:Adenosine Triphosphate; D011433:Propranolol; D000242:Cyclic AMP; D010732:Phosphofructokinase-1; D000254:Sodium-Potassium-Exchanging ATPase; D005947:Glucose; D010100:Oxygen; D011224:Prazosin; D004837:Epinephrine