title: Molecular analysis of Boophilus spp. (Acari: Ixodidae) tick strains.
abstract: Boophilus spp. (Acari: Ixodidae) parasitize cattle and other farm and wild animals in tropical and subtropical regions of the world. Ticks belonging to the genus Boophilus have undergone evolutionary processes associated with habitat adaptation following biogeographical separation, resulting in strains with marked morphological differences. We have characterized at the molecular level B. microplus strains from Latin America and Australia, employing sequences derived from the bm86 coding region, an intron located within the bm86 gene, and DNA short tandem repeats (STR). A B. annulatus strain was employed for comparison. The results indicated that variation within the bm86 coding region is higher between B. microplus strains than between some B. microplus strains and B. annulatus. The sequence of the intron was not informative for phylogenetic analysis, varying among individuals of the same strain. Two STRs were identified in B. microplus (STRs BmM1 and BmM2) and one in B. annulatus (STR Ba1). Southern hybridization experiments with STRs BmM1 and BmM2 as a probe revealed the prevalence of dispersed moderately repeated DNA in the genome of B. microplus. The analysis of polymorphism at STR locus BmM1 evidenced differences within and between populations of B. microplus. These results support at the molecular level the existing differences between B. microplus strains and suggest tools to characterize these populations.
authors.affiliation: Division of Mammalian Cell Genetics, Centro de Ingeniería Genética y Biotecnología. P.O. Box 6162, Havana, Cuba. jose_delafuente@yahoo.com
authors.forename: J
authors.initials: J
authors.lastname: Fuente
authors.forename: J C
authors.initials: JC
authors.lastname: García-García
authors.forename: D M
authors.initials: DM
authors.lastname: González
authors.forename: G
authors.initials: G
authors.lastname: Izquierdo
authors.forename: M E
authors.initials: ME
authors.lastname: Ochagavia
mesh_terms: D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000737:chemistry; D014644:Genetic Variation; D016678:Genome; D018884:Ixodes / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment
chemical_list: D004247:DNA