title: Application of PFGE performed with XbaI to an epidemiological and phylogenetic study of Salmonella serotype typhimurium. Relations between genetic types and phage types.
abstract: PFGE performed with XbaI was evaluated and applied as a typing method in a series of 68 Salmonella serotype Typhimurium strains collected in a Spanish region throughout 1984-1994, and four reference strains. Using bands > 100 kb as a separation criterion, 26 pulsed-field profiles (PFPs) were defined, with a discrimination index of 0.87. The XbaI-profiles were grouped into three clusters and two additional branches in a dendrogram of similarity (significance level = 0.7). Strains belonging to PFP-X1 and PFP-X2 predominated (26.4% and 23.6%, respectively) and fell into the major cluster. The series had been previously analysed by phage typing and a three-way ribotyping procedure, and a certain degree of relation among the three methods was revealed, PFGE being the most discriminatory. Combining data from the three methods a further differentiation into 46 clonal lines was obtained, and four lines, at least, could be considered as endemic in the region under study. This procedure proved to be a powerful epidemiological tool for characterization of Typhimurium and in the investigation of outbreaks.
authors.affiliation: Departamento de Biología Funcional, Universidad de Oviedo, Facultad de Medicina, Spain.
authors.forename: B
authors.initials: B
authors.lastname: Guerra
authors.forename: P
authors.initials: P
authors.lastname: Schrörs
authors.forename: M C
authors.initials: MC
authors.lastname: Mendoza
mesh_terms: D015373:Bacterial Typing Techniques; D001434:Bacteriophage Typing; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D005069:Evaluation Studies as Topic; D006801:Humans; D010802:Phylogeny; D015183:Restriction Mapping; D012480:Salmonella Infections / Q000453:epidemiology* / Q000382:microbiology; D012483:Salmonella Phages; D012486:Salmonella typhimurium / Q000145:classification* / Q000235:genetics / Q000821:virology
chemical_list: C025580:endodeoxyribonuclease XBAI; D015252:Deoxyribonucleases, Type II Site-Specific