title: Isolation and partial characterization of the Kluyveromyces lactis homologue of SKP1.
abstract: The SKP1 gene of Kluyveromyces lactis was isolated as a suppressor of a lethal temperature-sensitive mutation in the Saccharomyces cerevisiae CTF13 gene (Chromosome Transmission Factor 13). KlSKP1 was localized at chromosome V, adjacent to KlPAS3. A similar arrangement of the two genes is present in S. cerevisiae. Disruption of the KISKP1 gene was lethal, whereas overexpression of KlSKP1 lead to a decreased growth rate, to swollen and chain-forming cells with an increased DNA content, and to decreased plasmid stability. In both yeasts, promoter constructs lacking most of the purported binding sequence showed increased transcription levels of KlSKP1 in comparison to constructs with the entire promoter.
authors.affiliation: Clusius Laboratory, Institute for Molecular Plant Sciences, Leiden University, The Netherlands.
authors.forename: A A
authors.initials: AA
authors.lastname: Winkler
authors.forename: R H
authors.initials: RH
authors.lastname: Goedegebure
authors.forename: B J
authors.initials: BJ
authors.lastname: Zonneveld
authors.forename: H Y
authors.initials: HY
authors.lastname: Steensma
authors.forename: P J
authors.initials: PJ
authors.lastname: Hooykaas
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000235:genetics*; D004268:DNA-Binding Proteins; D044783:F-Box Proteins; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics; D015321:Gene Rearrangement; D006801:Humans; D007438:Introns; D007716:Kluyveromyces / Q000235:genetics*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics; D010957:Plasmids; D015183:Restriction Mapping; D044786:S-Phase Kinase-Associated Proteins; D044843:SKP Cullin F-Box Protein Ligases; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; C081354:CTF13 protein, S cerevisiae; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D044783:F-Box Proteins; D005656:Fungal Proteins; D009687:Nuclear Proteins; D044786:S-Phase Kinase-Associated Proteins; D029701:Saccharomyces cerevisiae Proteins; D044843:SKP Cullin F-Box Protein Ligases; C120507:SKP1 protein, S cerevisiae