title: Successful storage of RBCs for 9 weeks in a new additive solution.
abstract: BACKGROUND
  This study explored the effect of storing packed RBCs suspended in 200 mL of an alkaline, hypotonic, experimental additive solution (EAS 61).
  
  
  METHODS
  Packed RBC units prepared from RBCs collected from healthy donors in CPD were stored for 8 (n = 10) and 9 (n = 10) weeks under blood bank conditions after the addition of 200 mL of EAS 61 (adenine, 2 mM:; dextrose, 110 mM:; mannitol, 55 mM:; NaCl, 26 mM:; Na(2)HPO(4), 12 mM:). Standard methods were used for in vitro assays. The 24-hour in vivo autologous recoveries were measured with (51)Cr.
  
  
  RESULTS
  Mean +/- SD recoveries at 8 and 9 weeks were 81 +/- 7 and 77 +/- 7 percent. After 9 weeks, the ATP of the RBCs was 81 percent of the initial value, hemolysis was 0.35 percent, supernatant potassium was 46 mEq per L, and the morphologic index was 94.1.
  
  
  CONCLUSIONS
  Packed RBCs suspended in 200 mL of EAS 61 can be stored satisfactorily for 9 weeks. Longer RBC storage should reduce outdating, increase availability of transfusions in remote locations, and improve the efficiency of autologous donor programs.
authors.affiliation: Blood Research Detachment, Walter Reed Army Institute of Research, Washington, DC 20307-5100, USA. John.Hess@AMEDD.Army.Mil
authors.forename: J R
authors.initials: JR
authors.lastname: Hess
authors.forename: N
authors.initials: N
authors.lastname: Rugg
authors.forename: A D
authors.initials: AD
authors.lastname: Knapp
authors.forename: J F
authors.initials: JF
authors.lastname: Gormas
authors.forename: E B
authors.initials: EB
authors.lastname: Silberstein
authors.forename: T J
authors.initials: TJ
authors.lastname: Greenwalt
mesh_terms: D000328:Adult; D001782:Blood Donors; D001793:Blood Preservation; D004912:Erythrocytes; D005260:Female; D006801:Humans; D007038:Hypotonic Solutions / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D009996:Osmotic Fragility / Q000187:drug effects / Q000502:physiology; D013997:Time Factors
chemical_list: D007038:Hypotonic Solutions