Inhibition by dietary tea polyphenols of chemical mediator release from rat peritoneal exudate cells.
Male Wistar rats were given purified diets containing safflower (SAF), perilla (PER), or palm (PAL) oils with or without 1% tea polyphenols (TP) for 3 weeks, and chemical mediator releasing activity from rat peritoneal exudate cells (PEC) was measured. Histamine releasing activity was not influenced by TP, while histamine release and intracellular histamine content were significantly increased in the PAL-fed group. On the contrary, leukotriene B4 (LTB4) release was significantly lower in rats fed PER than in those fed SAF and PAL, and TP significantly decreased the release in all fat groups. TP also significantly inhibited the release of LTB5, which was generated only in rats fed PER. TP significantly decreased the proportion of arachidonic acid (AA) in PEC in the SAF-fed group and that of eicosapentaenoic acid (EPA), the precursor of LTB5 in the PER-fed group, but did not influence that of AA in the PAL- and PER-fed group. These results suggest that ingestion of TP improves type I allergic symptom through the inhibition of LT release though the inhibition by TP could not be totally explained by the reduction of substrate fatty acid.
Matsuo
N
N
Department of Biochemistry, Oita Medical University, Japan. matsuon@oita-med.ac.jp
Yamada
K
K
Mori
M
M
Shoji
K
K
Ueyama
T
T
Yunoki
S
S
Yamashita
K
K
Ozeki
M
M
Sugano
M
M
D000818:Animals; D002478:Cells, Cultured; D004041:Dietary Fats; D019587:Dietary Supplements; D004435:Eating; D015118:Eicosapentaenoic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D005419:Flavonoids; D006128:Growth; D006636:Histamine Release; D007975:Leukotriene B4 / Q000031:analogs & derivatives* / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008297:Male; D009929:Organ Size; D010537:Peritoneum / Q000166:cytology; D010636:Phenols / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism; D011108:Polymers / Q000378:metabolism*; D059808:Polyphenols; D051381:Rats; D017208:Rats, Wistar; D013662:Tea
D004041:Dietary Fats; D005227:Fatty Acids; D005419:Flavonoids; D010636:Phenols; D010743:Phospholipids; D011108:Polymers; D059808:Polyphenols; D013662:Tea; D007975:Leukotriene B4; C040212:leukotriene B5; D015118:Eicosapentaenoic Acid