'Glowing' chromosomes in cells undergoing rapid division.
Previous investigations in which metaphase plates of cells in rapid division were incubated in phosphate buffer at high temperature revealed numerous heterochromatic dots in chromosomes after Giemsa staining. In contrast, chromosomes from cells with a reduced capacity for reproduction were devoid of such dots, or the dots were sloughed-off into rings and patches of heterochromatin. In two types of cells which were rapidly dividing, namely HeLa cells (cervical cancer) and cells from regenerating planaria, phosphate incubation followed by Giemsa staining revealed an 'aura' or 'glowing' effect on the chromosomes, consisting of a densely staining core surrounded by a lightly stained periphery. This finding might be developed into a diagnostic test for certain malignancies, for cells undergoing dedifferentiation, or for tissues undergoing regeneration.
Edelman
J R
JR
Department of Science, Borough of Manhattan Community College, City University of New York, New York 10007, USA.
Lin
Y J
YJ
D000818:Animals; D001399:Azure Stains / Q000494:pharmacology; D002455:Cell Division / Q000235:genetics; D002875:Chromosomes / Q000737:chemistry / Q000187:drug effects* / Q000648:ultrastructure; D006367:HeLa Cells / Q000166:cytology / Q000648:ultrastructure; D006570:Heterochromatin / Q000187:drug effects; D006651:Histocytochemistry; D006801:Humans; D008677:Metaphase; D010932:Planarians / Q000166:cytology / Q000648:ultrastructure
D001399:Azure Stains; D006570:Heterochromatin