Anti-GM1 antibody facilitates leakage in an in vitro blood-nerve barrier model.
Using an in vitro blood-nerve barrier (BNB) model, the authors tested the effect of various monoclonal antiganglioside antibodies on BNB function. Only anti-GM1 antibody significantly facilitated BNB leakage in a concentration-dependent, complement-independent manner. This study provided evidence that anti-GM1 antibody, frequently detected in sera from patients with inflammatory neuropathies, may participate BNB dysfunction and contribute to development of neuropathy.
Kanda
T
T
Department of Neurology and Neuroscience, Tokyo Medical and Dental University Graduate School of Medicine, Japan. t-kanda.nuro@med.tmd.ac.jp
Iwasaki
T
T
Yamawaki
M
M
Tai
T
T
Mizusawa
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D000918:Antibody Specificity; D001812:Blood-Brain Barrier / Q000187:drug effects* / Q000276:immunology; D002250:Carbon Radioisotopes; D002417:Cattle; D002420:Cauda Equina / Q000098:blood supply* / Q000166:cytology; D002478:Cells, Cultured; D003165:Complement System Proteins / Q000494:pharmacology; D015194:Diffusion Chambers, Culture; D004305:Dose-Response Relationship, Drug; D017097:Electric Impedance; D016228:Endothelial Growth Factors / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D005677:G(M1) Ganglioside / Q000037:antagonists & inhibitors / Q000276:immunology*; D007444:Inulin / Q000493:pharmacokinetics; D008222:Lymphokines / Q000494:pharmacology; D008657:Metabolic Clearance Rate / Q000187:drug effects; D008954:Models, Biological; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D000911:Antibodies, Monoclonal; D002250:Carbon Radioisotopes; D016228:Endothelial Growth Factors; D008222:Lymphokines; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D005677:G(M1) Ganglioside; D007444:Inulin; D003165:Complement System Proteins