A mouse IgG1 monoclonal antibody specific for N-glycolyl GM3 ganglioside recognized breast and melanoma tumors.
14F7 murine monoclonal antibody (MAb) is an IgG1 immunoglobulin that is generated by immunizing Balb/c mice with GM3(NeuGc) ganglioside hydrophobically conjugated with human very-low-density lipoproteins and in the presence of Freund's adjuvants. 14F7 MAb binds specifically to GM3(NeuGc), whereas neither N-glycolyl or N-acetyl gangliosides, nor a sulfated glycolipid, are recognized as assessed by enzyme-linked immunosorbent assay or immunostaining on thin layer chromatograms. Immunohistochemical studies in fresh tumor tissues showed that 14F7 MAb strongly recognized in antigen expressed in human breast and melanoma tumors.
Carr
A
A
Center of Molecular Immunology, Havana, Cuba. adriana@ict.cim.sld.cu
Mullet
A
A
Mazorra
Z
Z
Vázquez
A M
AM
Alfonso
M
M
Mesa
C
C
Rengifo
E
E
Pérez
R
R
Fernández
L E
LE
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D000918:Antibody Specificity; D014408:Biomarkers, Tumor / Q000276:immunology*; D001943:Breast Neoplasms / Q000737:chemistry / Q000276:immunology*; D015243:Cholesterol, VLDL / Q000276:immunology / Q000378:metabolism; D005260:Female; D005679:G(M3) Ganglioside / Q000031:analogs & derivatives* / Q000276:immunology* / Q000378:metabolism; D006017:Glycolipids / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry / Q000378:metabolism*; D008545:Melanoma / Q000737:chemistry / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009928:Organ Specificity
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D015243:Cholesterol, VLDL; D005679:G(M3) Ganglioside; D006017:Glycolipids; D007074:Immunoglobulin G; C055763:de-N-acetylneuraminyllactosylceramide; C044298:N-glycolylneuraminyllactosylceramide