Application of a chromogenic medium and the PCR method for the rapid confirmation of L. monocytogenes in foodstuffs.
Detection of Listeria monocytogenes in foodstuffs by conventional cultivation methods carried out according to EN ISO guidelines is rather time-consuming. Therefore, two alternative methods were applied for rapid confirmation of L. monocytogenes in foodstuffs. Inoculum from liquid selective broth was plated on PALCAM and OXFORD agar and on chromogenic agar medium RAPID L. mono. Suspect colonies from PALCAM were confirmed according to EN ISO standards and by the multiplex PCR method. In total, 990 samples of foodstuffs were investigated and 63 strains of L. monocytogenes were isolated. The chromogenic medium RAPID L. mono provided results comparable to PCR, it is easier to handle and provides considerable financial savings.
Karpísková
R
R
National Institute of Public Health Prague, Centre of Food Chains Hygiene, Palackeho 1-3, 612 42, Brno, Czech Republic. karpi@chpr.szu.cz
Pejchalová
M
M
Mokrosová
J
J
Vytrasová
J
J
Smuharová
P
P
Ruprich
J
J
D002863:Chromogenic Compounds; D003470:Culture Media / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D008089:Listeria monocytogenes / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012703:Serotyping; D013045:Species Specificity
D002863:Chromogenic Compounds; D003470:Culture Media; D004269:DNA, Bacterial