Growth of rotaviruses in continuous human and monkey cell lines that vary in their expression of integrins.
Rotavirus replication occurs in vivo in intestinal epithelial cells. Cell lines fully permissive to rotavirus include kidney epithelial (MA104), colonic (Caco-2) and hepatic (HepG2) types. Previously, it has been shown that cellular integrins alpha 2 beta 1, alpha 4 beta 1 and alpha X beta 2 are involved in rotavirus cell entry. As receptor usage is a major determinant of virus tropism, the levels of cell surface expression of these integrins have now been investigated by flow cytometry on cell lines of human (Caco-2, HepG2, RD, K562) and monkey (MA104, COS-7) origin in relation to cellular susceptibility to infection with monkey and human rotaviruses. Cells supporting any replication of human rotaviruses (RD, HepG2, Caco-2, COS-7 and MA104) expressed alpha 2 beta 1 and (when tested) alpha X beta 2, whereas the non-permissive K562 cells did not express alpha 2 beta 1, alpha 4 beta 1 or alpha X beta 2. Only RD cells expressed alpha 4 beta 1. Although SA11 grew to higher titres in RD, HepG2, Caco-2, COS-7 and MA104 cells, this virus still replicated at a low level in K562 cells. In all cell lines tested, SA11 replicated to higher titres than did human strains, consistent with the ability of SA11 to use sialic acids as alternative receptors. Levels of cell surface alpha 2 integrin correlated with levels of rotavirus growth. The alpha 2 integrin relative linear median fluorescence intensity on K562, RD, COS-7, MA104 and Caco-2 cells correlated linearly with the titre of SA11 produced in these cells at 20 h after infection at a multiplicity of 0.1, and the data best fitted a sigmoidal dose-response curve (r(2)=1.00, P=0.005). Thus, growth of rotaviruses in these cell lines correlates with their surface expression of alpha 2 beta 1 integrin and is consistent with their expression of alpha X beta 2 and alpha 4 beta 1 integrins.
Londrigan
Sarah L
SL
Department of Microbiology and Immunology, The University of Melbourne, Parkville 3052, Victoria, Australia1.
Hewish
Marilyn J
MJ
Department of Microbiology and Immunology, The University of Melbourne, Parkville 3052, Victoria, Australia1.
Thomson
Melanie J
MJ
Department of Microbiology and Immunology, The University of Melbourne, Parkville 3052, Victoria, Australia1.
Sanders
Georgina M
GM
Department of Microbiology and Immunology, The University of Melbourne, Parkville 3052, Victoria, Australia1.
Mustafa
Huseyin
H
Department of Gastroenterology and Clinical Nutrition, The Royal Children's Hospital, Parkville 3052, Victoria, Australia2.
Coulson
Barbara S
BS
Department of Gastroenterology and Clinical Nutrition, The Royal Children's Hospital, Parkville 3052, Victoria, Australia2.|Department of Microbiology and Immunology, The University of Melbourne, Parkville 3052, Victoria, Australia1.
D000818:Animals; D019556:COS Cells; D018938:Caco-2 Cells; D005434:Flow Cytometry; D000882:Haplorhini; D006801:Humans; D016023:Integrins / Q000096:biosynthesis*; D007413:Intestinal Mucosa / Q000821:virology; D051379:Mice; D012401:Rotavirus / Q000254:growth & development*; D013499:Surface Properties; D014162:Transfection; D014779:Virus Replication
D016023:Integrins