title: Dietary fish oil reduces microvascular thrombosis in a porcine experimental model.
abstract: Microvascular thrombosis plays a significant role in the pathophysiology of ischaemic reperfusion injury. A fish oil-supplemented diet containing n-3 polyunsaturated fatty acids (PUFA) reduces thromboxane A(2) (TxA(2)) synthesis and, thus, vasoconstriction and platelet aggregation. The aim of this study was to elucidate whether n-3 PUFA in a porcine model of ischaemia and reperfusion injury 1) inhibit accumulation of platelets and fibrinogen in ischaemia-reperfusion injured tissue, 2) prolong the bleeding time, and 3) inhibit TxA(2) synthesis. Nine pigs were fed a standard diet supplemented with 7 g n-3 PUFA/day for 3 weeks. Nine pigs on the standard diet served as controls. Unilateral myocutaneous flaps were exposed to ischaemia for a period of 6 hours. Contralateral flaps were nonischaemic. Tissue contents of radioactive-labelled platelets and fibrinogen were measured after 4 hours of reperfusion. Platelet count, serum TxB(2), and the cutaneous bleeding time were measured before and after 3 weeks of diet. In the fish oil group, the accumulation of platelets was significantly reduced in all the myocutaneous flaps, except in the ischaemic skin part, when compared to control animals. Fibrinogen was significantly reduced in nonischaemic flaps, but not in ischaemic flaps. After the feeding period, the level of TxB(2) was significantly lowered in the fish oil group (p<0.01). No difference in the bleeding time was observed. Thus, dietary supplementation with n-3 PUFA inhibits the formation of microvasculatory thrombosis in this model.
authors.affiliation: Institute of Experimental Clinical Research, University of Aarhus, Aarhus, Denmark.
authors.forename: M
authors.initials: M
authors.lastname: Thorwest
authors.forename: E
authors.initials: E
authors.lastname: Balling
authors.forename: S D
authors.initials: SD
authors.lastname: Kristensen
authors.forename: S
authors.initials: S
authors.lastname: Aagaard
authors.forename: A
authors.initials: A
authors.lastname: Hakami
authors.forename: S E
authors.initials: SE
authors.lastname: Husted
authors.forename: J
authors.initials: J
authors.lastname: Marqversen
authors.forename: V E
authors.initials: VE
authors.lastname: Hjortdal
mesh_terms: D000818:Animals; D001760:Bleeding Time; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology; D004195:Disease Models, Animal; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology; D005340:Fibrinogen / Q000187:drug effects / Q000378:metabolism; D005395:Fish Oils / Q000494:pharmacology*; D008833:Microcirculation / Q000503:physiopathology*; D010976:Platelet Count / Q000187:drug effects; D015427:Reperfusion Injury / Q000097:blood / Q000473:pathology; D013552:Swine; D013927:Thrombosis / Q000517:prevention & control*; D013929:Thromboxane B2 / Q000097:blood
chemical_list: D004042:Dietary Fats, Unsaturated; D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D013929:Thromboxane B2; D005340:Fibrinogen