title: Binding kinetics of triclosan (Irgasan) to alloplastic vascular grafts: an in vitro study.
abstract: The aim of this study was to investigate the binding kinetics of triclosan (Irgasan) to alloplastic vascular grafts and to examine its antimicrobial activity against various microbial pathogens in vitro. Vascular grafts made by Intergard (Intervascular), Fluoropassiv (Vascutek), and Gore-tex (Gore) were examined. Grafts were incubated in 10 g/L triclosan (Irgasan), dried, sterilized, and incubated in RPMI medium. One-centimeter segments of the grafts were resected under sterile conditions at intervals of minutes, then hours, followed by days and up to 4 weeks. Samples were stored frozen at -20 degrees C for the measurement of triclosan bound to the vascular graft by high-performance liquid chromatography (HPLC). The binding kinetics under perfusion conditions were determined for Intergard grafts, which were perfused with 50 mL of nutrient medium for 24 hr. Samples were taken at various time intervals for the measurement of triclosan. The antimicrobial activity of triclosan against Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans as well as Enterococcus faecium was determined. Triclosan effectively binds to vascular graft without the use of intermediate binding substances. It stayed on the graft for the duration of 4 weeks. Under both static and perfusion conditions, the binding kinetics are similar. Triclosan binds most effectively to Intergard grafts, less so to Fluoropassiv grafts, and not at all to Gore-tex material. Antimicrobial activity of triclosan is very effective against S. aureus and E. faecium but not against P. aeruginosa.
authors.affiliation: Chirurgische Klinik und Poliklinik, Klinikum Grosshadern, Ludwig Maximilians-Universität München, Munich, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Hernández-Richter
authors.forename: H M
authors.initials: HM
authors.lastname: Schardey
authors.forename: F
authors.initials: F
authors.lastname: Löhlein
authors.forename: C T
authors.initials: CT
authors.lastname: Fleischer
authors.forename: A K
authors.initials: AK
authors.lastname: Walli
authors.forename: K S
authors.initials: KS
authors.lastname: Boos
authors.forename: F W
authors.initials: FW
authors.lastname: Schildberg
mesh_terms: D000891:Anti-Infective Agents, Local; D001807:Blood Vessel Prosthesis; D002176:Candida albicans / Q000187:drug effects; D020099:Coated Materials, Biocompatible; D016984:Enterococcus faecium / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D008826:Microbial Sensitivity Tests; D016459:Prosthesis-Related Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D014260:Triclosan
chemical_list: D000891:Anti-Infective Agents, Local; D020099:Coated Materials, Biocompatible; D014260:Triclosan