[IL-1 and TNF-alpha-mediated regulation of IL-6, IL-8, and GM-CSF release from cultured nasal epithelial cells].
IL-6, IL-8 and GM-CSF regulate inflammatory cell infiltration and survival at sites of inflammation. IL-1, TNF-alpha, IL-6, IL-8 and GM-CSF have been reported to be released from cultured nasal epithelial cells (CNEC). Here, focusing on IL-1 and TNF-alpha as key cytokines, we examined whether these two cytokines could regulate the release of IL-6, IL-8 and GM-CSF from CNEC. CNEC were treated with antibodies to IL-1 alpha and TNF-alpha at various doses for a period of 48 hrs. The levels of IL-6, IL-8 and GM-CSF in the culture supernatants were then estimated by ELISA. Treatment of CNEC with antibodies to IL-1 alpha and TNF-alpha inhibited the release of IL-6, IL-8 and GM-CSF from them in a dose-dependent manner. The inhibition of IL-6 and GM-CSF release was significantly greater after treatment of the CNEC with the antibodies to IL-1 alpha. Furthermore CNEC expressed IL-1 receptors and TNF receptors. These results suggest that the epithelial cell-derived IL-1 alpha and TNF-alpha can regulate the release of other cytokines like IL-6, IL-8 and GM-CSF in an autocrine manner.
Yamagishi
S
S
Department of Otolaryngology, Nippon Medical School, Tokyo.
Ohnishi
M
M
Pawankar
R
R
D000328:Adult; D019898:Autocrine Communication; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism*; D006801:Humans; D007375:Interleukin-1 / Q000502:physiology*; D015850:Interleukin-6 / Q000378:metabolism*; D016209:Interleukin-8 / Q000378:metabolism*; D008297:Male; D009297:Nasal Mucosa / Q000166:cytology / Q000378:metabolism*; D017472:Receptors, Interleukin-1 / Q000378:metabolism; D018124:Receptors, Tumor Necrosis Factor / Q000378:metabolism; D012221:Rhinitis, Allergic, Perennial / Q000209:etiology; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology*
D007375:Interleukin-1; D015850:Interleukin-6; D016209:Interleukin-8; D017472:Receptors, Interleukin-1; D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha; D016178:Granulocyte-Macrophage Colony-Stimulating Factor