title: Acellular hemoglobin solution enters compressed lung capillaries more readily than red blood cells.
abstract: High lung inflation pressures compress alveolar septal capillaries, impede red cell transit, and interfere with oxygenation. However, recently introduced acellular hemoglobin solutions may enter compressed lung capillaries more easily than red blood cells. To test this hypothesis, we perfused isolated rat lungs with fluorescently labeled diaspirin cross-linked hemoglobin (DCLHb; 10%) and/ or autologous red cells (hematocrit, 20). Septal capillaries were compressed by setting lung inflation pressure above vascular pressures (zone 1). Examination by confocal microscopy showed that DCLHb was distributed throughout alveolar septa. Furthermore, this distribution was not affected by adding red blood cells to the perfusate. We estimated the maximum acellular hemoglobin mass within septa to be equivalent to that of 15 red blood cells. By comparison, we found an average of 2.7 +/- 4.6 red cells per septum in zone 1. These values increased to 30.4 +/- 25.8 and 50.4 +/- 22.1 cells per septum in zones 2 and 3, respectively. We conclude that perfusion in zone 1 with a 10% acellular hemoglobin solution may increase the hemoglobin concentration per septum up to fivefold compared with red cell perfusion.
authors.affiliation: Department of Surgery, University of Wisconsin-Madison, and William S. Middleton Memorial Veterans Hospital, Madison, Wisconsin 53705, USA.
authors.forename: R L
authors.initials: RL
authors.lastname: Conhaim
authors.forename: L A
authors.initials: LA
authors.lastname: Rodenkirch
authors.forename: K E
authors.initials: KE
authors.lastname: Watson
authors.forename: B A
authors.initials: BA
authors.lastname: Harms
mesh_terms: D000818:Animals; D001241:Aspirin / Q000031:analogs & derivatives* / Q000493:pharmacokinetics*; D002196:Capillaries / Q000378:metabolism; D002199:Capillary Permeability; D004912:Erythrocytes / Q000502:physiology*; D006454:Hemoglobins / Q000493:pharmacokinetics*; D066298:In Vitro Techniques; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D011650:Pulmonary Alveoli / Q000378:metabolism; D011652:Pulmonary Circulation; D051381:Rats; D014018:Tissue Distribution
chemical_list: D006454:Hemoglobins; C083478:diaspirin-cross-linked hemoglobin; D001241:Aspirin