title: Chemiluminescence derivatization of methylglyoxal using 2-aminonicotinic acid.
abstract: To develop a sensitive and selective chemiluminometric method for the determination of methylglyoxal, we used 2-aminonicotinic acid as the chemiluminescence derivatization reagent. 2-Aminonicotinic acid reacts with methylglyoxal in an acidic solution at 37 degrees C for 4 h and gave a chemiluminescence in N,N'-dimethylformamide containing sodium tert-butoxide. The detection limit (blank intensity plus three-times its standard deviation of methylglyoxal) for methylglyoxal is 233 fmol in the reaction mixture.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyushu University, Higashi, Fukuoka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Ohba
authors.forename: M
authors.initials: M
authors.lastname: Sakata
authors.forename: K
authors.initials: K
authors.lastname: Zaitsu
mesh_terms: D007700:Kinetics; D008163:Luminescent Measurements; D009539:Nicotinic Acids / Q000737:chemistry*; D011765:Pyruvaldehyde / Q000737:chemistry*; D013696:Temperature
chemical_list: D009539:Nicotinic Acids; C110415:2-aminonicotinic acid; D011765:Pyruvaldehyde