Evaluation of osteoblast response to porous bioactive glass (45S5) substrates by RT-PCR analysis.
Previous studies have shown that neonatal rat calvaria osteoblasts elaborate substantial amounts of extracellular material with bone-like characteristics when cultured on porous bioactive glass substrates in vitro. However, the osteoblastic response to this material has not been fully characterized. The objective of this study was to characterize osteoblast response to porous bioactive glass substrates following the expression of the classical markers for osteoblast differentiation. In this study we synthesized porous bioactive glass substrates, seeded them with osteoblast-like cells (ROS 17/2.8) and followed the temporal expression of alkaline phosphatase (AP) activity, as well as the expression of mRNA for collagen type I (Coll-1), osteonectin (OSN), osteopontin (OPN), osteocalcin (OCN), and bone sialoprotein (BSP). The data confirm that porous bioactive glass substrates are capable of supporting the in vitro growth and maturation of osteoblast-like cells. At a porosity of 42% and an average pore size of 80 microm, the substrates promote the expression and maintenance of the osteoblastic phenotype. The results additionally suggest that there is both a solution-mediated and a surface-controlled effect on cell activity.
Effah Kaufmann
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Department of Bioengineering, School of Dental Medicine, Center for Bioactive Materials and Tissue Engineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
Ducheyne
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Shapiro
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D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D001672:Biocompatible Materials; D001698:Biomedical Engineering; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D003094:Collagen / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D005898:Glass; D058574:Integrin-Binding Sialoprotein; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology*; D015675:Osteocalcin / Q000235:genetics; D015676:Osteonectin / Q000235:genetics; D053495:Osteopontin; D010641:Phenotype; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012795:Sialoglycoproteins / Q000235:genetics
D001672:Biocompatible Materials; D017931:DNA Primers; C546873:Ibsp protein, rat; D058574:Integrin-Binding Sialoprotein; D015676:Osteonectin; D012333:RNA, Messenger; D012795:Sialoglycoproteins; C506897:Spp1 protein, rat; D015675:Osteocalcin; D053495:Osteopontin; D003094:Collagen; D000469:Alkaline Phosphatase