Specific preinduction of 60-kDa heat shock protein (chaperonin homolog) by TRH does not protect colonic mucosa against acetic acid-induced lesion in rats.
In order to study the cytoprotective function of colonic heat shock proteins (HSPs) in vivo, the effect of specific preinduction of HSP60 by thyrotropin-releasing hormone (TRH) administration on the development of acetic acid-induced colonic mucosal lesion was investigated. Expression of 60-kDa, 72-kDa, and 90-kDa heat shock proteins (HSP60, HSP72, and HSP90, respectively) in rat colonic mucosa was investigated by western blot and immunohistochemical analyses before and after TRH administration. Following pretreatment with or without TRH administration, the rats received intrarectal infusion of 5% acetic acid. The colonic mucosal damage was macroscopically evaluated 24 hr after the intrarectal infusion of acetic acid. Expression of HSP60 was significantly increased by TRH administration in the colonic mucosa, whereas HSP72 and HSP90 did not increase. Immunohistochemical study also showed a significant increase in HSP60 in colonic mucosal cells, especially at the surface of the colonic mucosa after TRH administration. No histopathologic alteration was observed in the colonic mucosa after TRH administration. The colonic mucosal damage caused by intrarectal infusion of 5% acetic acid was not prevented by preinduction of HSP60. We demonstrated that specific preinduction of HSP60 by TRH administration did not show cytoprotective function in the colonic mucosa, although this protein plays a crucial role for cytoprotection in the pancreatic acinar cells. Our results indicate that the role of HSP60 may be different in each organ with respect to cytoprotection.
Iwabuchi
A
A
Department of Internal Medicine-1, Akita University School of Medicine, Akita City, Japan.
Otaka
M
M
Otani
S
S
Jin
M
M
Okuyama
A
A
Itoh
S
S
Sasahara
H
H
Odashima
M
M
Ivan
P
P
Itoh
H
H
Tashima
Y
Y
Masamune
O
O
Watanabe
S
S
D019342:Acetic Acid / Q000494:pharmacology*; D000818:Animals; D018834:Chaperonin 60 / Q000378:metabolism*; D003106:Colon / Q000187:drug effects* / Q000473:pathology; D003108:Colonic Diseases / Q000139:chemically induced* / Q000473:pathology / Q000517:prevention & control*; D004305:Dose-Response Relationship, Drug; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000473:pathology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013973:Thyrotropin-Releasing Hormone / Q000494:pharmacology*
D018834:Chaperonin 60; D013973:Thyrotropin-Releasing Hormone; D019342:Acetic Acid