Vacuole acidification is not required for survival of Salmonella enterica serovar typhimurium within cultured macrophages and epithelial cells.
Phagosome acidification is an important component of the microbicidal response by infected eukaryotic cells. Thus, intracellular pathogens that reside within phagosomes must either block phagosome acidification or be able to survive at low pH. In this work, we studied the effect of phagosomal acidification on the survival of intracellular Salmonella enterica serovar Typhimurium in different cell types. Bafilomycin A1, a specific inhibitor of the vacuolar proton-ATPases, was used to block acidification of salmonella-containing vacuoles. We found that in several epithelial cell lines, treatment with bafilomycin A1 had no effect on intracellular survival or replication. Furthermore, although acidification was essential for Salmonella intracellular survival in J774 cultured macrophages, as reported previously (13), it is not essential in other macrophage cell lines. These data suggest that vacuolar acidification may play a role in intracellular survival of salmonellae only under certain conditions and in specific cell types.
Steele-Mortimer
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Biotechnology Laboratory, Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia V6T 1Z3.
St-Louis
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Olivier
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Finlay
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D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000382:microbiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D018942:Macrolides; D008264:Macrophages / Q000382:microbiology*; D051379:Mice; D006180:Proton-Translocating ATPases / Q000502:physiology; D012486:Salmonella typhimurium / Q000502:physiology*; D014617:Vacuoles / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D018942:Macrolides; C040929:bafilomycin A1; D006180:Proton-Translocating ATPases