title: Broad spectrum antimicrobial activity of a new multi-purpose disinfecting solution.
abstract: OBJECTIVE
  There has been increasing awareness of the need for more powerful contact lens disinfectants, ideally having low toxicity and allergenicity to ocular tissue. Opti-Free Express with ALDOX Multi-Purpose Disinfecting Solution (MPDS) was recently marketed as a new multi-purpose disinfecting solution for soft contact lenses. MPDS contains two antimicrobial agents, polyquaternium-1 and myristamidopropyl dimethylamine, to broaden the range of antimicrobial activity. The purpose of this study was to evaluate the antimicrobial activity of MPDS against microorganisms that have been reported as contact lens and lens case contaminants and causative agents of microbial keratitis.
  
  
  METHODS
  MPDS was challenged with high numbers of gram-positive and gram-negative bacteria, yeast, mold, and Acanthamoeba. Several lots of product were challenged with at least three separate inocula of each isolate. After exposure to the disinfecting solution, a sample was removed from the product and plated for survivors by suitable recovery methods. The plates were incubated and the number of survivors was enumerated.
  
  
  RESULTS
  The results showed that MPDS was bactericidal, fungicidal and amoebicidal against an extensive variety of environmental contaminants of lens care accessories and ocular pathogens. MPDS was capable of killing a broad spectrum of microorganisms. The log reduction exceeded the primary criteria for disinfecting products and produced a notable reduction of other clinical and environmental isolates as well.
  
  
  CONCLUSIONS
  Opti-Free Express MPDS contains antimicrobial agents that can kill an extensive spectrum of many different types of microorganisms that may contaminate contact lenses and solutions.
authors.affiliation: Alcon Laboratories, Ltd, Fort Worth, TX 76134-2099, USA.
authors.forename: R A
authors.initials: RA
authors.lastname: Rosenthal
authors.forename: C L
authors.initials: CL
authors.lastname: McAnally
authors.forename: L S
authors.initials: LS
authors.lastname: McNamee
authors.forename: S L
authors.initials: SL
authors.lastname: Buck
authors.forename: R L
authors.initials: RL
authors.lastname: Schlitzer
authors.forename: R P
authors.initials: RP
authors.lastname: Stone
mesh_terms: D000048:Acanthamoeba / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D000818:Animals; D001419:Bacteria / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D015169:Colony Count, Microbial; D018639:Contact Lens Solutions / Q000494:pharmacology*; D004202:Disinfectants / Q000494:pharmacology*; D004338:Drug Combinations; D015817:Eye Infections / Q000517:prevention & control; D005658:Fungi / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D006801:Humans; D008826:Microbial Sensitivity Tests; D021261:Parasitic Sensitivity Tests; D011108:Polymers / Q000494:pharmacology*; D011437:Propylamines / Q000494:pharmacology*
chemical_list: D018639:Contact Lens Solutions; D004202:Disinfectants; D004338:Drug Combinations; D011108:Polymers; D011437:Propylamines; C058655:polyquaternium 1; C401661:myristamidopropyl dimethylamine