title: Co-repressor complexes and remodelling chromatin for repression.
abstract: Recent progress identifies targeted chromatin remodelling by co-repressor complexes as being an integral component of transcriptional silencing. Here we discuss how chromatin structure and the basal transcriptional machinery are manipulated by the co-repressor complex containing the Mi-2 nucleosomal ATPase, the histone-binding protein RbAp48 and histone deacetylase and by the co-repressor complex containing SIN3, RbAp48 and histone deacetylase. Remarkably, both of these complexes also contain methyl-CpG-binding proteins. This observation provides a molecular mechanism to integrate DNA methylation fully into gene control in vertebrates.
authors.affiliation: Laboratory of Molecular Embryology, National Institute of Child Heath and Human Development, NIH, Building 18T, Room 106, Bethesda, MD 20892-5431, USA. awlme@helix.nih.gov
authors.forename: A P
authors.initials: AP
authors.lastname: Wolffe
authors.forename: F D
authors.initials: FD
authors.lastname: Urnov
authors.forename: D
authors.initials: D
authors.lastname: Guschin
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D002843:Chromatin / Q000737:chemistry* / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone; D002875:Chromosomes / Q000378:metabolism; D018899:CpG Islands / Q000235:genetics; D019175:DNA Methylation; D020868:Gene Silencing; D006655:Histone Deacetylases / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D006801:Humans; D008954:Models, Biological; D009687:Nuclear Proteins / Q000378:metabolism; D009707:Nucleosomes / Q000378:metabolism; D011987:Receptors, Steroid / Q000378:metabolism*; D012097:Repressor Proteins; D056505:Retinoblastoma-Binding Protein 4; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D015533:Transcriptional Activation
chemical_list: D002352:Carrier Proteins; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D006657:Histones; D009687:Nuclear Proteins; D009707:Nucleosomes; C082637:RBBP4 protein, human; D011987:Receptors, Steroid; D012097:Repressor Proteins; D056505:Retinoblastoma-Binding Protein 4; C071731:SIN3 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D006655:Histone Deacetylases; D000251:Adenosine Triphosphatases