title: Determination of the sensitivity of a rapid Escherichia coli O157:H7 assay for testing 375-gram composite samples.
abstract: Both 25-g single-size ground beef samples and 375-g composite ground beef samples were tested by a method combining an immunomagnetic separation (IMS) technique with a sandwich enzyme-linked immunosorbent assay (ELISA) system (IMS-ELISA). The results demonstrated that IMS-ELISA could detect the target, Escherichia coli O157:H7, at the level of 10(-1) CFU/g of sample in either the 25- or 375-g sample size.
authors.affiliation: Richter International, Inc., Columbus, Ohio 43201, USA.
authors.forename: W L
authors.initials: WL
authors.lastname: Tsai
authors.forename: C E
authors.initials: CE
authors.lastname: Miller
authors.forename: E R
authors.initials: ER
authors.lastname: Richter
mesh_terms: D000818:Animals; D002417:Cattle; D015169:Colony Count, Microbial; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D019453:Escherichia coli O157 / Q000302:isolation & purification*; D005516:Food Microbiology; D018189:Immunomagnetic Separation / Q000379:methods*; D008461:Meat Products / Q000382:microbiology*; D012680:Sensitivity and Specificity