Antioxidant effect of oral dipyridamole during cerebral hypoperfusion with human carotid endarterectomy.
We sought to test the hypothesis of an antioxidant effect of dipyridamole in vivo in a model of cerebral hypoperfusion. Twenty-one patients (65+/-10 years, 11 men) undergoing carotid endarterectomy were allocated in two groups (group 1, 10 with dipyridamole, 200 mg p.o., 3-4 h before surgery; group II, 11 with placebo) in a double-blind placebo-controlled randomized design. Blood was sampled from ipsilateral jugular bulb, and plasma vitamin E content was assayed before, after 15 and 30 min of clamp, and 2 and 10 min after declamping. In 12 of them, lipoperoxides were assayed. Vitamin E plasma content decreased significantly in group II (rest, 3.71+/-0.22 mmol/mol of cholesterol, 100%) after clamp (91.5% of rest, p < 0.01) and remained unchanged during declamping (90.9% of rest, p < 0.01), but did not change in group I (rest, 3.5+/-0.44 mmol/mol of cholesterol, 100%) during clamping (99.9% of rest; p = NS) and after declamping (97.6% of rest; p = NS). Lipoperoxide concentration did not change in group I (rest, 302+/-8 a.u.; clamp, 296+/-13 a.u.; p = NS vs. rest; declamp, 304+/-8 a.u.; p = NS vs. rest), and increased significantly in group II (rest, 313+/-5 a.u.; clamp, 352+/-9 a.u.; p < 0.01 vs. rest; declamp, 343+/-6 a.u.; p < 0.05 vs. rest). Cerebral oxidative stress associated with human carotid endarterectomy can be attenuated by pretreatment with oral dipyridamole.
Kusmic
C
C
Institute of Clinical Physiology, CNR, Pisa, Italy. kusmic@ifc.pi.cnr.it
Petersen
C
C
Picano
E
E
Busceti
C
C
Parenti
G
G
Pasini
F L
FL
Barsacchi
R
R
D000368:Aged; D000975:Antioxidants / Q000494:pharmacology*; D002560:Cerebrovascular Circulation / Q000187:drug effects*; D002784:Cholesterol / Q000097:blood; D004176:Dipyridamole / Q000494:pharmacology*; D004311:Double-Blind Method; D016894:Endarterectomy, Carotid / Q000009:adverse effects*; D005073:Evoked Potentials, Somatosensory / Q000187:drug effects; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008875:Middle Aged; D017585:Ultrasonography, Doppler, Transcranial; D014665:Vasodilator Agents / Q000494:pharmacology*; D014810:Vitamin E / Q000097:blood
D000975:Antioxidants; D014665:Vasodilator Agents; D014810:Vitamin E; D004176:Dipyridamole; D002784:Cholesterol