title: N-glycosylated variants of growth hormone in human pituitary extracts.
abstract: This study was designed to investigate the existence, in human pituitary extracts, of growth hormone (GH) variants not encoded by the hGH-N gene. Using anion exchange-fast protein liquid chromatography followed by SDS-PAGE, we isolated several basic forms of pituitary GH. Incubation of these basic forms with endoglycosidase F/N-glycosidase F revealed that two of them (about 34 and 12 kD) were N-glycosylated. In contrast, no changes were found when samples were incubated with the O-linked glycosylation-specific O-glycosidase. Since the GH-N molecule lacks consensus sequences for N-linked glycosylation, our findings suggest that GH genes other than hGH-N are expressed in the human pituitary gland.
authors.affiliation: Departamento de Fisiología, Facultad de Medicina, Santiago de Compostela, España.
authors.forename: M
authors.initials: M
authors.lastname: García-Barros
authors.forename: J A
authors.initials: JA
authors.lastname: Costoya
authors.forename: R
authors.initials: R
authors.lastname: Ríos
authors.forename: V
authors.initials: V
authors.lastname: Arce
authors.forename: J
authors.initials: J
authors.lastname: Devesa
mesh_terms: D000595:Amino Acid Sequence; D015870:Gene Expression; D014644:Genetic Variation; D006031:Glycosylation; D019382:Human Growth Hormone / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D005810:Multigene Family; D010902:Pituitary Gland / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid
chemical_list: D019382:Human Growth Hormone