title: AZFa deletions in Sertoli cell-only syndrome: a retrospective study.
abstract: Lack of data on the genotype-phenotype relationship in cases of AZF microdeletions is due to the limited number of histological investigations in human male infertility cases. We investigated the possibility of retrospective detection of Yq11 microdeletions by using DNA extracted from diagnostic testicular biopsies. We used histological criteria to select two series of material: 22 biopsies with Sertoli cell-only syndrome and 14 biopsies with maturation arrest at the spermatocyte I stage. Two markers, DFFRY and DAZ, were tested by nested polymerase chain reaction (PCR) in the two series. In the Sertoli cell-only syndrome series, we found four deletions affecting the DFFRY gene (18.2%). In the second series, no deletions were detected. Two conclusions may be considered, although the number of specimens analysed is limited: (i) the frequency of deletions observed in Sertoli cell-only syndrome allows us to suggest that deletion in the AZFa region may be involved in this pathology; and (ii) retrospective studies may yield some additional elements in our search for eventual genotype-phenotype relationships.
authors.affiliation: Service de Cytogénétique-Immunocytologie-Biologie du Développement et de la Reproduction, CHU et CNRS UPRESA 6025, Faculté de Médecine et de Pharmacie, Place Saint-Jacques, 25030 Besançion cedex, France. oxana_blagoski@hotmail.com
authors.forename: O
authors.initials: O
authors.lastname: Blagosklonova
authors.forename: F
authors.initials: F
authors.lastname: Fellmann
authors.forename: M C
authors.initials: MC
authors.lastname: Clavequin
authors.forename: C
authors.initials: C
authors.lastname: Roux
authors.forename: J L
authors.initials: JL
authors.lastname: Bresson
mesh_terms: D002872:Chromosome Deletion; D000075364:Deleted in Azoospermia 1 Protein; D006182:H-Y Antigen / Q000235:genetics*; D006801:Humans; D008297:Male; D009845:Oligospermia / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000235:genetics*; D012189:Retrospective Studies; D012708:Sertoli Cells; D013577:Syndrome; D013737:Testis / Q000473:pathology; D014998:Y Chromosome
chemical_list: C095668:DAZ1 protein, human; D000075364:Deleted in Azoospermia 1 Protein; D006182:H-Y Antigen; D016601:RNA-Binding Proteins