Rapid extracellular acidification induced by glucose metabolism in non-proliferating cells of Serratia marcescens.
The addition of glucose or other sugars to resting cells of Serratia maurcescens induced rapid acidification of the extracellular medium. This acidification was due to the catabolism of sugars. The rate of acidification depended on the carbon source and its concentration. HPLC analysis of the supernatants demonstrated that the progressive fall in pH resulted from the rapid production of lactic, acetic, pyruvic and citric acids. Other microorganisms were tested for their ability to produce this rapid acidification of the medium. This study may provide a rapid and simple method for metabolism studies.
Solé
M
M
Department of Medical Microbiology and Parisitology, University of Barcelona, Spain.
Rius
N
N
Lorén
J G
JG
D019342:Acetic Acid / Q000378:metabolism; D050260:Carbohydrate Metabolism; D002851:Chromatography, High Pressure Liquid; D019343:Citric Acid / Q000378:metabolism; D017077:Culture Media, Conditioned / Q000737:chemistry; D004734:Energy Metabolism; D005947:Glucose / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000378:metabolism; D011353:Prodigiosin / Q000096:biosynthesis; D019289:Pyruvic Acid / Q000378:metabolism; D012706:Serratia marcescens / Q000378:metabolism*; D013402:Sugar Alcohols / Q000378:metabolism
D017077:Culture Media, Conditioned; D013402:Sugar Alcohols; D019343:Citric Acid; D019344:Lactic Acid; D019289:Pyruvic Acid; D005947:Glucose; D011353:Prodigiosin; D019342:Acetic Acid