Demonstration of Cl- requirement for inhibition of vacuolar acidification by cycloprodigiosin in situ.
Applying vacuole-perfusion and plasma membrane permeabilization techniques to internodal cells of Chara, we analyzed the requirement of Cl- for the action of cycloprodigiosin (cPrG) to inhibit vacuole acidification in situ. By combining the two techniques, the Cl- concentration on both sides of the tonoplast could be controlled. In permeabilized cell fragments lacking Cl- in the vacuole, the inhibitory effect of cPrG on vacuole acidification was cancelled. On the other hand, Cl- in the cytoplasm was not needed for the cPrG action. These results supported the function of cPrG as a H+/Cl- symporter. Requirement of Cl- for the cPrG action was also demonstrated in vacuole-perfused living cells. This is the first report on the mechanism of cPrG action in situ.
Nakayasu
T
T
Department of Life Science, Faculty of Science, Himeji Institute of Technology, Harima Science Park City, Hyogo, Japan.
Kawauchi
K
K
Hirata
H
H
Shimmen
T
T
D000143:Acids / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism*; D007211:Indoles / Q000494:pharmacology*; D011758:Pyrroles / Q000494:pharmacology*; D014617:Vacuoles / Q000187:drug effects* / Q000378:metabolism
D000143:Acids; D002712:Chlorides; D007211:Indoles; D011758:Pyrroles; C108328:cycloprodigiosin