title: Comparison of Vero cell assay, polymerase chain reaction and an enzyme immunoassay for identification of verocytotoxin-producing Escherichia coli O157:H7.
abstract: This study evaluated three different analytical methods for identification of Verocytotoxin-producing E. coli O157:H7 (VTEC) strains. A total of 34 E. coli O157:H7 strains isolated from bovine faeces and bovine carcasses were comparatively tested with Vero cell assay (VCA), PCR and the sandwich ELISA "RIDASCREEN Verotoxin" test. The VCA, performed without a neutralization assay, gave a false positive result because a VCA-positive E. coli O157:H7 strain did not possess the VT-coding genes when tested with PCR. The lack of specificity of the VCA could be avoided by testing for neutralization of cytotoxicity. The commercial ELISA system was as sensitive and specific as PCR, with the advantages of being a more rapid and easier procedure which could be employed in all first level diagnostic laboratories.
authors.affiliation: Istituto di Ispezione degli Alimenti di origine animale, Facoltà di Medicina Veterinaria, Università di Parma, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Bonardi
authors.forename: E
authors.initials: E
authors.lastname: Foni
authors.forename: E
authors.initials: E
authors.lastname: Maggi
mesh_terms: D000003:Abattoirs; D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis*; D002417:Cattle / Q000382:microbiology*; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay; D019453:Escherichia coli O157 / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D005243:Feces / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D022622:Shiga Toxin 1; D014709:Vero Cells
chemical_list: D001427:Bacterial Toxins; D011933:Reagent Kits, Diagnostic; D022622:Shiga Toxin 1