Characterization of gene expression of NsERFs, transcription factors of basic PR genes from Nicotiana sylvestris.
Three genes of NsERFs (EREBPs), transcription factors for GCC box of basic PR genes, were isolated from Nicotiana sylvestris. Analyses of transgenic tobacco carrying the NsERF promoter::GUS genes showed that expression of all NsERF genes in leaves was induced by ethylene. Sequence analyses indicated that the 5'-upstream region of NsERF3 gene has the GCC box. In contrast, the promoter regions of NsERF2 and 4 have no GCC box, whereas NsERF2 has a putative EIN3 binding site. Tissue/cell specific expression is also discussed.
Kitajima
S
S
Radioisotope Research Center, Kyoto University, Japan.
Koyama
T
T
Ohme-Takagi
M
M
Shinshi
H
H
Sato
F
F
D000595:Amino Acid Sequence; D001483:Base Sequence; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005966:Glucuronidase / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; C053376:pathogenesis-related proteins, plant; D005966:Glucuronidase