Inhibition of gastric mucosal damage by methylglyoxal pretreatment in rats.
The effect of methylglyoxal pretreatment on gastric mucosal injuries caused by 80% ethanol, 25% NaCl and 0.2 M NaOH, was investigated in rats. The effects caused by pylorous ligation accumulated gastric acid secretions and ethanol-induced changes in gastric mucus secretions, levels of proteins, nucleic acid, malondialdehyde (MDA) and non-protein sulfhydryl groups were also investigated. Methylglyoxal pretreatment at oral doses of 50, 100 and 200 mg/kg body weight was found to provide a dose-dependent protection against the ulcerogenic effects of different necrotizing agents used. With the same dose regimen methylglyoxal offered significant protection against ethanol-induced damage on the parameters evaluated for histopathology. Furthermore, the pretreatment afforded a dose-dependent inhibition of pylorous ligated accumulation of gastric acid secretions and ethanol-induced depletion of stomach wall mucus, proteins, nucleic acids, NP-SH contents and an increase in the MDA levels in gastric tissue. The protective effect of methylglyoxal against ethanol-induced damage to the gastric wall mucosa may be mediated through its effect on mucous production, proteins, nucleic acids, NP-SH groups and its free-radical scavenging property under the influence of polyamines stimulated by ornithine decarboxylase activity (ODC).
Al-Shabanah
O A
OA
Department of Pharmacology, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
Qureshi
S
S
Al-Harbi
M M
MM
Al-Bekairi
A M
AM
Al-Gharably
N M
NM
Raza
M
M
D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D000431:Ethanol; D005744:Gastric Acid / Q000378:metabolism; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D009696:Nucleic Acids / Q000378:metabolism; D009955:Ornithine Decarboxylase / Q000378:metabolism; D011073:Polyamines / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D011765:Pyruvaldehyde / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012965:Sodium Chloride; D012972:Sodium Hydroxide; D013276:Stomach Ulcer / Q000139:chemically induced* / Q000517:prevention & control; D013438:Sulfhydryl Compounds / Q000378:metabolism
D000897:Anti-Ulcer Agents; D009696:Nucleic Acids; D011073:Polyamines; D011506:Proteins; D013438:Sulfhydryl Compounds; D000431:Ethanol; D012965:Sodium Chloride; D008315:Malondialdehyde; D012972:Sodium Hydroxide; D011765:Pyruvaldehyde; D009955:Ornithine Decarboxylase