Isolation of cDNA clones corresponding to genes expressed during fruit ripening in Japanese pear (Pyrus pyrifolia Nakai): involvement of the ethylene signal transduction pathway in their expression.
Thirty cDNA clones of genes corresponding to mRNAs up-regulated during fruit ripening of Japanese pear (Pyrus pyrifolia Nakai cv. Kikusui) were obtained by differential screening of a ripe fruit cDNA library. All of these cDNAs were sequenced and gathered into 11 non-redundant groups after database searches. These clones represented genes associated with stress responses, protein catabolism or pathogenesis. The accumulation of transcripts of 3 out of 11 genes was inhibited by 1-methylcyclopropene (MCP), an inhibitor of ethylene action.
Itai
A
A
Laboratory of Horticultural Science, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan. itai@muses.tottor-u.ac.jp
Tanabe
K
K
Tamura
F
F
Tanaka
T
T
D015152:Blotting, Northern; D018076:DNA, Complementary / Q000302:isolation & purification; D005030:Ethylenes / Q000378:metabolism*; D005638:Fruit / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D018749:RNA, Plant / Q000032:analysis; D019685:Rosales / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D015398:Signal Transduction
D018076:DNA, Complementary; D005030:Ethylenes; D012333:RNA, Messenger; D018749:RNA, Plant; C036216:ethylene