Detection and investigation of Campylobacter jejuni by polymerase chain reaction-restriction fragment length polymorphism analysis.
A molecular typing approach for Campylobacter jejuni with restriction fragment length polymorphism (RFLP) analysis of the flagellin gene flaA in C. jejuni, was generated and studied. Using polymerase chain reaction (PCR)-RFLP with the restriction endonuclease Mbo I, it was demonstrated that C. jejuni could be divided into four types. Genotypic analysis of C. jejuni by PCR-RFLP is a valuable technique for epidemiological typing.
Kiuchi
A
A
Department of Veterinary Microbiology, School of Veterinary Medicine, Azabu University, Sagamihara Kanagawa, Japan.
Hara
M
M
Pham
H S
HS
Takikawa
K
K
Itoh
R
R
Tabuchi
K
K
D000818:Animals; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D002169:Campylobacter Infections / Q000382:microbiology / Q000662:veterinary*; D016123:Campylobacter jejuni / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D002645:Chickens; D004269:DNA, Bacterial / Q000032:analysis; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D005408:Flagellin / Q000235:genetics; D005838:Genotype; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011201:Poultry Diseases / Q000382:microbiology*
D004269:DNA, Bacterial; D005408:Flagellin; D015252:Deoxyribonucleases, Type II Site-Specific; C024405:GATC-specific type II deoxyribonucleases