title: Mast cell granule composition and tissue location--a close correlation.
abstract: This review provides a survey on mast cell heterogeneity, with aspects differing in humans and rodents or which are subject of conflicting evidence being discussed in greater detail. Mast cell subsets have been first defined in rats by their fixation and dye-binding properties, and detailed studies in humans and pigs reveal very similar observations. The dye-binding properties of rat mast cell subsets are causally related to the absence or presence of heparin in their granules. In humans, this relation has not been shown. Rodent mast cell subsets store different chymase-isoforms. In contrast, just a single chymase has been defined in humans, and mast cells are classified by the presence or relative absence of this chymase. Different investigators find quite different proportions of chymase-positive to chymase-negative mast cells. Tryptase(s) are found in most or every human mast cell, but in rodents, they have hitherto been essentially localised to mast cells in connective tissues. Human mast cell subsets may also be defined by their expression of receptors such as C5aR and possibly the beta-chemokine receptor CCR3; the CCR3 expression seems to be related to the human mast cell chymase expression. Ultrastructural studies are helpful to distinguish human mast cell subsets, and allow to distinguish between chronic and acute activation. The phenotypical characteristics may change in association with inflammation or other disease processes. Studies in humans and pigs show changed dye-binding and fixation properties of the granules. Experimental rodent infection models reveal similar changes of chymase isoform expression. Human lung mast cells have been reported to strongly upregulate their chymase content in pulmonary vascular disease. This line of evidence can explain some inconsistent information on mast cell heterogeneity and may help to understand the physiological role of mast cells.
authors.affiliation: Department of Pathology, Vienna University, Austria.
authors.forename: W J
authors.initials: WJ
authors.lastname: Beil
authors.forename: M
authors.initials: M
authors.lastname: Schulz
authors.forename: U
authors.initials: U
authors.lastname: Wefelmeyer
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D053818:Chymases; D004396:Coloring Agents / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism; D006801:Humans; D016131:Lymphocyte Subsets; D008407:Mast Cells / Q000276:immunology / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D009092:Mucous Membrane / Q000166:cytology; D011971:Receptors, Immunologic; D012697:Serine Endopeptidases / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods
chemical_list: D004396:Coloring Agents; D006025:Glycosaminoglycans; D011971:Receptors, Immunologic; D010450:Endopeptidases; D012697:Serine Endopeptidases; D053818:Chymases