Reverse micelles as life-mimicking systems.
In this review, we attempt to demonstrate that reverse micelles are simple artificial systems that mimic many life systems from cell division to the creation of an enzyme catalytic mechanism. For a membranous enzyme like placental alkaline phosphatase, the kinetic properties observed in reverse micelles might represent those found under physiological conditions. The reverse micellar system, consisting of a positively charged surfactant, mimics a detoxification enzyme glutathione transferase. We propose a novel island-in-oil-lake reverse micellar model for the glutathione transferase that can account for almost all the catalytic properties of this enzyme. Reverse micelles may provide an excellent model system in investigating the reaction mechanism of other detoxification enzymes.
Chang
G G
GG
Graduate Institutes of Biochemistry and Life Sciences, National Defense Medical Center, Taipei, Taiwan, ROC.
Huang
T M
TM
Hung
H C
HC
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D008566:Membranes / Q000502:physiology; D008823:Micelles; D008954:Models, Biological; D012313:RNA / Q000235:genetics; D012997:Solvents; D013501:Surface-Active Agents
D008823:Micelles; D012997:Solvents; D013501:Surface-Active Agents; D012313:RNA; D005982:Glutathione Transferase; D000469:Alkaline Phosphatase