title: Immunization with recombinant surface antigens p26 with Freund's adjuvants against Babesia rodhaini infection.
abstract: The surface proteins of Babesia rodhaini have previously been shown to induce a high degree of protective immunity. In the present study, one of those proteins, B. rodhaini antigen p26 was expressed in Escherichia coli and in insect cells infected with a recombinant baculovirus. These proteins were recognized by immune serum from a drug-cured BALB/c mouse. While BALB/c mice immunized with both recombinant antigens and Freund's adjuvants showed 40-100% survival rate against challenge infection with B. rodhaini, saponin failed to induce protection, although significant levels of B. rodhaini-specific antibodies were produced in both immunized mice (1:1,000-2,000 by indirect immunofluorescent antibody test). The immunization of IFN-gamma-deficient mice with the recombinant proteins was not protective against B. rodhaini infection, indicating that IFN-gamma is one of the important factors for the survival against lethal B. rodhaini infection.
authors.affiliation: The Research Center for Protozoan Molecular Immunology, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Igarashi
authors.forename: U
authors.initials: U
authors.lastname: Asaba
authors.forename: X
authors.initials: X
authors.lastname: Xuan
authors.forename: Y
authors.initials: Y
authors.lastname: Omata
authors.forename: A
authors.initials: A
authors.lastname: Saito
authors.forename: H
authors.initials: H
authors.lastname: Nagasawa
authors.forename: K
authors.initials: K
authors.lastname: Fujisaki
authors.forename: N
authors.initials: N
authors.lastname: Suzuki
authors.forename: Y
authors.initials: Y
authors.lastname: Iwakura
authors.forename: T
authors.initials: T
authors.lastname: Mikami
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D001403:Babesia / Q000276:immunology*; D001404:Babesiosis / Q000276:immunology* / Q000517:prevention & control; D003001:Cloning, Molecular; D004912:Erythrocytes / Q000469:parasitology; D004926:Escherichia coli; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005620:Freund's Adjuvant; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D019444:Vaccines, DNA / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D007074:Immunoglobulin G; D019444:Vaccines, DNA; D005620:Freund's Adjuvant