title: Peroxisomal membrane fusion requires two AAA family ATPases, Pex1p and Pex6p.
abstract: Two AAA family ATPases, NSF and p97, have been implicated in membrane fusion during assembly and inheritance of organelles of the secretory pathway. We have now investigated the roles of AAA ATPases in membrane fusion during assembly of the peroxisome, an organelle outside the classical secretory system. Here, we show that peroxisomal membrane fusion in the yeast Yarrowia lipolytica requires two AAA ATPases, Pex1p and Pex6p. Release of membrane- associated Pex1p and Pex6p drives the asymmetric priming of two fusion partners. The next step, peroxisome docking, requires release of Pex1p from one partner. Subsequent fusion of the peroxisomal membranes is independent of both Pex1p and Pex6p.
authors.affiliation: Department of Cell Biology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada.
authors.forename: V I
authors.initials: VI
authors.lastname: Titorenko
authors.forename: R A
authors.initials: RA
authors.lastname: Rachubinski
mesh_terms: D000074183:ATPases Associated with Diverse Cellular Activities; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D007425:Intracellular Membranes / Q000502:physiology*; D008561:Membrane Fusion / Q000502:physiology*; D008954:Models, Biological; D020675:Peroxisomes / Q000502:physiology*; D013104:Spheroplasts / Q000502:physiology; D015003:Yeasts / Q000201:enzymology / Q000502:physiology
chemical_list: D000251:Adenosine Triphosphatases; D000074183:ATPases Associated with Diverse Cellular Activities; C096998:Pex6 protein, rat