Alternative exon usage of rat septins.
Septins represent a family of phylogenetically conserved proteins required for cytokinesis. Their presence in pre- and postsynaptic neuronal membranes suggests a general function as scaffolds for membrane reorganization. The transcriptional regulation of all septins examined so far is complex, resulting in alternatively spliced variants. We focus here on the rat homologue of the gene for the human septin MSF, a truncated form of which, designated eseptin, had been described previously. It will be shown here that there is an alternative usage of the first exon by two forms, named exon r1a and r1b, respectively. Exon r1a, but not exon r1b, contains a part of the coding sequence while the start of translation for the remaining coding sequence resides in the second exon. The complete genomic organization was resolved and data on the temporal and spatial expression of this septins are presented.
Jackisch
B O
BO
Department of Internal Medicine, Institute of Physiological Chemistry and Pathobiochemistry, Münster, D-48149, Germany.
Hausser
H
H
Schaefer
L
L
Kappler
J
J
Müller
H W
HW
Kresse
H
H
D017398:Alternative Splicing / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003598:Cytoskeletal Proteins / Q000737:chemistry / Q000235:genetics*; D005091:Exons / Q000235:genetics*; D005347:Fibroblasts; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins / Q000737:chemistry / Q000235:genetics; D017403:In Situ Hybridization; D007668:Kidney / Q000378:metabolism; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D058112:Septins; D017386:Sequence Homology, Amino Acid
D003598:Cytoskeletal Proteins; D016376:Oligonucleotides, Antisense; D020033:Protein Isoforms; D012333:RNA, Messenger; C119785:Septin9 protein, rat; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; C474315:SEPTIN9 protein, human; D058112:Septins