title: Presence of phosphorylcholine on a filarial nematode protein influences immunoglobulin G subclass response to the molecule by an interleukin-10-dependent mechanism.
abstract: The filarial nematode product ES-62 contains phosphorylcholine (PC) covalently attached to N-linked glycans. ES-62 induced high levels of immunoglobulin G1 (IgG1) antibodies, but no IgG2a, to non-PC epitopes of the molecule following subcutaneous injection into BALB/c mice. Conversely, mice given ES-62 lacking PC demonstrated significant production of both IgG subclasses. Thus, PC appears to block production of IgG2a antibodies to other epitopes on the parasite molecule. A role for interleukin-10 (IL-10) in this effect was shown by the ability of IL-10(-/-) mice to make an IgG2a antibody response to non-PC epitopes of ES-62.
authors.affiliation: Department of Immunology, University of Strathclyde, Glasgow G4 0NR, United Kingdom.
authors.forename: K M
authors.initials: KM
authors.lastname: Houston
authors.forename: E H
authors.initials: EH
authors.lastname: Wilson
authors.forename: L
authors.initials: L
authors.lastname: Eyres
authors.forename: F
authors.initials: F
authors.lastname: Brombacher
authors.forename: M M
authors.initials: MM
authors.lastname: Harnett
authors.forename: J
authors.initials: J
authors.lastname: Alexander
authors.forename: W
authors.initials: W
authors.lastname: Harnett
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D005370:Filarioidea / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood / Q000145:classification*; D016753:Interleukin-10 / Q000502:physiology*; D015847:Interleukin-4 / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D010767:Phosphorylcholine / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D007074:Immunoglobulin G; D010767:Phosphorylcholine; D016753:Interleukin-10; D015847:Interleukin-4