Sensitive enzyme-linked immunosorbent assays for the detection of bacterial superantigens and antibodies against them in human plasma.
Enzyme-linked immunosorbent assays for the quantitation of bacterial superantigens, staphylococcal enterotoxins A, B and C, toxic-shock syndrome toxin-1 and streptococcal pyrogenic exotoxin A, were developed. The assays had sensitivity to quantitate these toxins to 1.4, 5.9, 16.3, 2.5 and 4.3 pg/ml, respectively, in a buffer including 50% human plasma. It takes only 150 min to complete the assays after plate preparation. Specificity of the assays agreed with those of reverse latex agglutination assay. We also developed enzyme-linked immunosorbent assays to detect antibodies against these five superantigens. The assays are expected to be significant tools for the study of superantigens in several diseases.
Miwa
K
K
Pioneering Research Laboratories, Toray Industries, Inc., Otsu, Shiga, Japan. Keishi Miwa@nts.toray.co.jp
Fukuyama
M
M
Sakai
R
R
Shimizu
S
S
Ida
N
N
Endo
M
M
Igarashi
H
H
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood* / Q000276:immunology; D000942:Antigens, Bacterial / Q000097:blood* / Q000276:immunology; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004768:Enterotoxins / Q000097:blood / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005098:Exotoxins / Q000097:blood / Q000276:immunology; D006801:Humans; D008565:Membrane Proteins; D051379:Mice; D011817:Rabbits; D012680:Sensitivity and Specificity; D013211:Staphylococcus aureus / Q000276:immunology / Q000302:isolation & purification; D013297:Streptococcus pyogenes / Q000276:immunology / Q000302:isolation & purification; D018089:Superantigens / Q000097:blood* / Q000276:immunology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004768:Enterotoxins; D005098:Exotoxins; D008565:Membrane Proteins; C117980:SpeA protein, Streptococcus pyogenes; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal; C027951:erythrogenic toxin; C026066:enterotoxin A, Staphylococcal; C031818:enterotoxin B, staphylococcal; C034959:enterotoxin C, staphylococcal