Dose dependent biological effects of idarubicin in HL-60 cells: alterations of the cell-cycle and apoptosis.
TP-53 deficient cells of human leukaemia HL-60 die by massive apoptosis after treatment by high (50-100 nmol/l) doses of DNA damaging agent Idarubicin, regardless of the cell-cycle phase, in which they are affected. In contrary, after relatively low dose 10 nmol/l the cells die after cell-cycle arrest in G2 phase. The results show, that apoptosis induced by idarubicin could appear independently of the cell-cycle phase and that period in which apoptosis is observed is related to the dose of Idarubicin.
Mareková
M
M
Department of Medical Biochemistry, Faculty of Medicine Hradec Králové, Charles University, Prague. marekova@lfhk.cuni.cz
Vávrová
J
J
Vokurková
D
D
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D018922:HL-60 Cells; D006801:Humans; D015255:Idarubicin / Q000494:pharmacology*
D000903:Antibiotics, Antineoplastic; D015255:Idarubicin