Simultaneous accumulation of low-molecular-mass RNA at the interface along with accumulation of high-molecular-mass RNA on aqueous two-phase system partitioning.
On partitioning in the potassium phosphate-PEG aqueous two-phase system, the simultaneous accumulation of the low-molecular-mass RNA at the interface along with accumulation of the coexisting high-molecular-mass RNA was quantitatively shown. The low-molecular-mass RNA was inherently partitioned between the top and bottom phases if partitioned alone. However, the low-molecular-mass RNA was caught in the interface to a significant extent if partitioned with the coexisting high-molecular-mass RNA. The degree of the accumulation of low-molecular-mass RNA increased with an increase in the content of the coexisting high-molecular-mass RNA, while the high-molecular-mass RNA was mostly accumulated at the interface.
Kimura
K
K
Bioengineering Department, National Institute of Bioscience and Human-Technology, Tsukuba, Ibaraki, Japan.
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008970:Molecular Weight; D012313:RNA / Q000737:chemistry / Q000378:metabolism*; D012995:Solubility; D013499:Surface Properties; D014867:Water / Q000737:chemistry
D014867:Water; D012313:RNA