CD8+ cytotoxic T lymphocyte responses to lytic proteins of human herpes virus 8 in human immunodeficiency virus type 1-infected and -uninfected individuals.
T cell immunity to lytic proteins of herpesviruses is important in host control of infection. We have characterized the cytotoxic T lymphocyte (CTL) response to 5 human herpesvirus 8 (HHV-8) homologues of lytic proteins in HHV-8-seropositive individuals. HLA class I-restricted, CD8(+) CTL responses to >/=1 HHV-8 lytic protein were detected in all 14 HHV-8-seropositive study subjects tested, with or without human immunodeficiency virus type 1 (HIV-1) infection, but not in any of 5 HHV-8-seronegative individuals. Seven of these study subjects with both HHV-8 and HIV-1 infection had greater anti-CTL reactivity to glycoprotein H (open-reading frame 22) than did the 7 study subjects infected only with HHV-8. Moreover, there was a strong, inverse correlation between HIV-1 load and glycoprotein H-specific CTL lysis in the study subjects infected with both viruses. CTL reactivity to HHV-8 lytic proteins may be involved in host control of HHV-8-related diseases, such as Kaposi's sarcoma.
Wang
Q J
QJ
Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA.
Jenkins
F J
FJ
Jacobson
L P
LP
Meng
Y X
YX
Pellett
P E
PE
Kingsley
L A
LA
Kousoulas
K G
KG
Baghian
A
A
Rinaldo
C R
CR
D017088:AIDS-Related Opportunistic Infections / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology; D015331:Cohort Studies; D003602:Cytotoxicity, Immunologic; D005260:Female; D005822:Genetic Vectors; D018023:HIV Seronegativity / Q000276:immunology*; D015497:HIV-1; D019288:Herpesvirus 8, Human; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D006801:Humans; D016130:Immunophenotyping; D008297:Male; D016366:Open Reading Frames; D012514:Sarcoma, Kaposi / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects* / Q000276:immunology; D014616:Vaccinia virus; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology; D019562:Viral Load
D000956:Antigens, Viral; D015395:Histocompatibility Antigens Class I; D014759:Viral Envelope Proteins; C116207:glycoprotein B, human herpesvirus 8