Decreased pulmonary levels of the anti-inflammatory Clara cell 16 kDa protein after induction of airway inflammation in asthmatics.
The Clara cell 16 kDa protein (CC16) maps to an atopy-associated region of chromosome 11 and has been ascribed an anti-inflammatory function. Using reverse-phase HPLC and Western blot analysis, we have evaluated the polypeptide pattern in bronchoalveolar lavage (BAL) fluid retrieved from asthmatics, before and after induction of airway inflammation by low-dose allergen inhalation challenge. A prominent decrease of CC16 was seen after induction of inflammation, and a further CC16 decrease was observed in lavage fluid where surfactant had been removed. Reduced levels of pulmonary CC16 may cause loss of anti-inflammatory activity in the airways and contribute to the development of airway inflammation in asthma.
Lensmar
C
C
Department of Medicine, Karolinska Hospital, Stockholm, Sweden. catarinal@lung.ks.se
Nord
M
M
Gudmundsson
G H
GH
Roquet
A
A
Andersson
O
O
Jörnvall
H
H
Eklund
A
A
Grunewald
J
J
Agerberth
B
B
D000418:Albumins / Q000032:analysis; D000485:Allergens / Q000276:immunology; D000893:Anti-Inflammatory Agents / Q000276:immunology / Q000378:metabolism; D001249:Asthma / Q000276:immunology / Q000378:metabolism* / Q000473:pathology* / Q000503:physiopathology; D015153:Blotting, Western; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007249:Inflammation / Q000276:immunology / Q000378:metabolism; D011506:Proteins / Q000276:immunology / Q000378:metabolism*; D011663:Pulmonary Surfactants / Q000378:metabolism; D012137:Respiratory System / Q000276:immunology / Q000378:metabolism* / Q000473:pathology* / Q000503:physiopathology; D014598:Uteroglobin; D000515:alpha 1-Antitrypsin / Q000378:metabolism
D000418:Albumins; D000485:Allergens; D000893:Anti-Inflammatory Agents; D011506:Proteins; D011663:Pulmonary Surfactants; C556389:SCGB1A1 protein, human; D000515:alpha 1-Antitrypsin; D014598:Uteroglobin