DNA fluorescent stain accumulates in the Golgi but not in the kinetosomes of amitochondriate protists.
Hindgut symbiotic trichomonads (uninucleate Caduceia versatilis, and multinucleate Stephanonympha sp. and Snyderella tabogae) from the dry-wood-eating termite Cryptotermes cavifrons (Kalotermitidae) accumulate DAPI (4,6diamidino-2-phenylindole) in the membranous sacs of the Golgi complex. This form of Golgi complex, typical of protists in the class Parabasalia, is called a parabasal body. Trichomonads contain organellar systems, mastigonts, that consist of four undulipodia (e.g. eukaryotic flagella and cilia), axostylar microtubules, a parabasal body and other structures. These cells bear from one (in the case of Caduceia) to hundreds (in the case of Snyderella) of mastigonts. These features are characteristic of their protist class (Parabasalia). The nuclei of all three species stained with DNA-specific stains: DAPI, SYTOX, acridine orange, propidium iodide, ethidium bromide and Feulgen, at optimal concentrations, but kinetosomes failed to stain at all. The nuclei, parabasal bodies and symbiotic bacteria (but no microtubular structures) fluoresced in glutaraldehyde-fixed cells stained with 1.45 microM DAPI. Parabasal bodies of Snyderella and Caduceia treated to remove lipids with Triton X-100, or treated with 5% trichloroacetic acid, lacked DAPI-fluorescence. I conclude that DNA, present as expected in nuclei and bacterial symbionts, is absent from and not associated with calonymphid kinetosomes. The reason for DNA-RNA stain accumulation in the Golgi cistemae is not clear.
Dolan
M F
MF
Graduate Program in Organismic and Evolutionary Biology, University of Massachusetts, Amherst 01003, USA. mdolan@geo.umass.edu
D000165:Acridine Orange / Q000378:metabolism; D000818:Animals; D004396:Coloring Agents / Q000378:metabolism; D016054:DNA, Protozoan / Q000032:analysis*; D004996:Ethidium / Q000378:metabolism; D005456:Fluorescent Dyes / Q000032:analysis / Q000378:metabolism*; D006056:Golgi Apparatus / Q000737:chemistry / Q000378:metabolism*; D007211:Indoles / Q000378:metabolism; D020049:Isoptera / Q000382:microbiology; D008856:Microscopy, Fluorescence; D015388:Organelles / Q000737:chemistry / Q000378:metabolism*; D009930:Organic Chemicals; D011419:Propidium / Q000378:metabolism; D012394:Rosaniline Dyes; D013559:Symbiosis; D016834:Trichomonadida / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology / Q000648:ultrastructure
D004396:Coloring Agents; D016054:DNA, Protozoan; C007722:Feulgen stain; D005456:Fluorescent Dyes; D007211:Indoles; D009930:Organic Chemicals; D012394:Rosaniline Dyes; C402795:SYTOX Green; D011419:Propidium; C007293:DAPI; D004996:Ethidium; D000165:Acridine Orange