title: Simple method of isolation and purification of alpha-galactosides from legumes.
abstract: A simple method for the isolation and purification of alpha-galactosides, raffinose family oligosaccharides (RFOs), from legumes has been developed. The method includes (i) imbibition of seeds, (ii) extraction with 50% ethanol, (iii) precipitation of RFOs, (iv) purification of RFOs on diatomaceous earth and charcoal, and (v) cation-exchange chromatography. The described method allows one to obtain high purity RFO preparations (90% for lentil and 80% for pea seeds, determined by HPLC-RI analysis) in the form of white, fine powder. Yields of alpha-galactosides isolated from 100 g of seeds of lentil and pea were 5.6 and 4.3 g, respectively.
authors.affiliation: Department of Biotechnology and Food Microbiology, Agricultural University, Poznań, Poland.
authors.forename: P
authors.initials: P
authors.lastname: Gulewicz
authors.forename: D
authors.initials: D
authors.lastname: Ciesiołka
authors.forename: J
authors.initials: J
authors.lastname: Frias
authors.forename: C
authors.initials: C
authors.lastname: Vidal-Valverde
authors.forename: S
authors.initials: S
authors.lastname: Frejnagel
authors.forename: K
authors.initials: K
authors.lastname: Trojanowska
authors.forename: K
authors.initials: K
authors.lastname: Gulewicz
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D007887:Fabaceae / Q000737:chemistry*; D005697:Galactosides / Q000302:isolation & purification*; D010946:Plants, Medicinal
chemical_list: D005697:Galactosides