Application of an anti-HQIgY antibody for the measurement of IgY concentrations of hen's and quail's serum and yolk.
The development of a sensitive ELISA for the measurement of quail IgY (QIgY) was the main purpose of our study. The suitable antibody (AB) was prepared in rabbits. Both quail IgY (QIgY) and hen IgY (HIgY) were precipitated by this developed AB. For this reason it was marked as anti-hen-quail-IgY (a-HQIgY). The purified AB was conjugated with horseradish peroxidase (aHQIgY-HRP) and a sensitive direct ELISA was developed, based on this labeled AB. The prepared aHQIgY AB which was used in this developed ELISA method was suitable for the measurement of total and specific IgY concentration in domestic hen (Gallus domesticus) and Japanese quail (Coturnix coturnix japonica) either. As a result of our experiments it is very likely that there are identical sequences of IgYs of both species. This part of IgY has good antigen character at the same time. Probably, this phenomenon has occurrence in other Galliform species, too. Further investigations will be carried out in this field.
Losonczy
S
S
Department of Animal Physiology and Health, University of Agricultural Sciences, Gödöllö, Hungary. animphys@tan.gau.hu
Szabó
C
C
Kiss
Z
Z
Bárdos
L
L
D000818:Animals; D002645:Chickens / Q000502:physiology*; D002846:Chromatography, Affinity; D003370:Coturnix; D004530:Egg Yolk / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D007120:Immunochemistry; D007136:Immunoglobulins / Q000032:analysis* / Q000276:immunology; D011784:Quail / Q000276:immunology*; D011817:Rabbits / Q000276:immunology
C016512:IgY; D007136:Immunoglobulins