Structural characterization and immunolocalization of egg antigens cross-react with Toxocara vitulorum, Fasciola gigantica and Moniezia expansa mature flukes.
A structural homology between eggs of Toxocara vitulorum, Fasciola gigantica and Moniezia expansa was proved by the use of SDS-PAGE. In immunoblot, 9, 11 and 7 polypeptides were recognized in F. gigantica, M. expansa and T. vitulorum eggs respectively by their respective rabbit anti-adult antisera. Moreover, components of 240 KD and 206 KD were recognized in the three eggs by different anti-adult antisera. The anatomic localization of the crossreactive epitopes in eggs was determined by indirect immunofluorescence microscopy. The cross-reactive epitopes were mainly associated with embryonted cells of F. gigantica, egg shell, larvae and vitelline membranes of T. vitulorum and egg shall and granular layer of M. expansa.
Abdel-Rahman
E H
EH
Department of Parasitology and Animal Diseases, National Research Center, Dokki, Giza, Egypt.
Abdel-Megeed
K N
KN
Hassanain
M A
MA
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D002589:Cestoda / Q000276:immunology*; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D005209:Fasciola / Q000276:immunology*; D010063:Ovum / Q000276:immunology; D011817:Rabbits; D014119:Toxocara / Q000276:immunology*
D000947:Antigens, Helminth