title: Determination of ebrotidine and its metabolites by capillary electrophoresis with UV and mass spectrometry detection.
abstract: This study describes the application of capillary electrophoresis (CE) to the analysis of ebrotidine and its metabolites as an alternative analytical technique to liquid chromatography. Comparison between UV-diode array spectroscopy and mass spectrometry (MS) using an ion-trap system with electrospray ionization as detection systems has been performed. The quality parameters of the UV detection method were established, obtaining linear calibration curves over the range studied (8-200 mg ml(-1)), limits of detection between 3.4 and 4.3 microg ml(-1), and run-to-run and day-to-day precision lower than 14%. For these compounds the protonated species [M+H]+ and, in some cases, sodium adducts were observed in the MS spectra. Using MS coupled to CE, limits of detection were between 0.5 and 2.6 microg ml(-1).
authors.affiliation: Department of Analytical Chemistry, University of Barcelona, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Sentellas
authors.forename: L
authors.initials: L
authors.lastname: Puignou
authors.forename: E
authors.initials: E
authors.lastname: Moyano
authors.forename: M T
authors.initials: MT
authors.lastname: Galceran
mesh_terms: D001557:Benzenesulfonates / Q000378:metabolism*; D002021:Buffers; D002412:Cations; D004573:Electrolytes; D019075:Electrophoresis, Capillary / Q000379:methods*; D006635:Histamine H2 Antagonists / Q000378:metabolism*; D013058:Mass Spectrometry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D013844:Thiazoles / Q000378:metabolism*
chemical_list: D001557:Benzenesulfonates; D002021:Buffers; D002412:Cations; D004573:Electrolytes; D006635:Histamine H2 Antagonists; D012997:Solvents; D013844:Thiazoles; C075156:ebrotidine