[Expression and plasma activity of plasminogen activator inhibitor-1 infibrotic liver tissues].
OBJECTIVE
To study plasminogen activator inhibitor-1 (PAI-1) gene expression and protein synthesis in fibrotic liver tissues and plasma activity levels.


METHODS
Plasma PAI-1 activity was detected by chromogenic activity assay kit. The messenger RNA (mRNA) of PAI-1 and PAI-1 protein was detected on serial biopsied liver specimens of normal, HBV-related chronic hepatitis and cirrhosis by in situ hybridization and immunohistochemistry. The semi-quantitative results of the protein expression to immunohistochemistry staining were analysed in contrast with the stage of hepatic fibrosis.


RESULTS
The plasma PAI-1 activity levels decreased with the progression of hepatic fibrosis. PAI-1 mRNA and protein were expressed in the extracellular matria deposition of the portal areas, focal necrotic areas, fibrous septa and hepatocytes as well as the hepatocellular cytoplasm adjacent to the areas described above.


CONCLUSIONS
Localization of PAI-1 is not only in interstitial cells but also hepatocytes, suggesting an intense relationship between PAI-1 and hepatic fibrosis.
Li
Q
Q
Department of Gastroenterology, Qingdao Municipal Hospital, Qingdao 266011, China.
Qin
C
C
Lao
P
P
D000293:Adolescent; D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008099:Liver / Q000378:metabolism*; D008103:Liver Cirrhosis / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D017395:Plasminogen Activator Inhibitor 1 / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis
D017395:Plasminogen Activator Inhibitor 1; D012333:RNA, Messenger