Genotypes of multidrug-resistant Salmonella enterica serotype typhimurium from two regions of Kenya.
A combination of phage typing and pulsed-field gel electrophoresis of Xbal-digested chromosomal DNA has been used to study the epidemiological relationships of multidrug-resistant Salmonella enterica serotype typhimurium from Nairobi (64 isolates) and Kilifi (40 isolates) collected over the period 1994-1997. Isolates from Nairobi belonged to 11 definitive phage types (DTs) encompassing eight different PFGE patterns. In contrast, isolates from Kilifi were mainly DT 56 (60%) and all fell into a single PFGE pattern. The remaining isolates did not conform to a recognisable phage type. We conclude that multidrug-resistant S. typhimurium infections from Nairobi were caused by multiple strains while those from Kilifi were likely to be from a microepidemic caused by a single clone.
Kariuki
S
S
Centre for Microbiology Research, Kenya Medical Research Institute, Nairobi. Skariuki@wtrl.or.ke
Oundo
J O
JO
Muyodi
J
J
Lowe
B
B
Threlfall
E J
EJ
Hart
C A
CA
D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001434:Bacteriophage Typing / Q000379:methods; D002648:Child; D002675:Child, Preschool; D003227:Conjugation, Genetic / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D018432:Drug Resistance, Multiple / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007630:Kenya / Q000453:epidemiology; D008826:Microbial Sensitivity Tests / Q000379:methods; D010957:Plasmids; D012480:Salmonella Infections / Q000453:epidemiology / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000145:classification* / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification
D000900:Anti-Bacterial Agents