title: Mutagenicity and genotoxicity of sorbic acid-amine reaction products.
abstract: Sorbic acid as well as potassium and calcium sorbate (E202 and E203) are legally used as preservatives in numerous processed foods. Owing to its system of conjugated double bonds, sorbic acid is likely to undergo a nucleophilic attack, which may turn it into mutagenic products. The cyclic derivatives resulting from a double addition reaction between sorbic acid and various amines at two different temperatures (50 degrees C and 80 degrees C) have been analysed. A genotoxicity study has been performed with HeLa cells and plasmid DNA. A mutagenesis study has been carried out by using the Ames test. A SOS spot test and a cytotoxicity study have been realised as well. The results showed that the products involved exhibited neither mutagenic nor genotoxic activities.
authors.affiliation: Laboratoire d'Analyse de la Valeur Biologique des Ailments, Département Universitaire des Sciences d'Agen, Université Bordeaux I, 47000 Agen, France. c.ferrand@dus-agen.u-bordeaux.fr
authors.forename: C
authors.initials: C
authors.lastname: Ferrand
authors.forename: F
authors.initials: F
authors.lastname: Marc
authors.forename: P
authors.initials: P
authors.lastname: Fritsch
authors.forename: P
authors.initials: P
authors.lastname: Cassand
authors.forename: G
authors.initials: G
authors.lastname: de Saint Blanquat
mesh_terms: D000588:Amines / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity*; D004247:DNA / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics; D005520:Food Preservatives / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity*; D018565:Food-Drug Interactions; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D009152:Mutagenicity Tests; D009153:Mutagens / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity*; D010957:Plasmids / Q000187:drug effects / Q000235:genetics; D013014:SOS Response, Genetics / Q000187:drug effects; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D013011:Sorbic Acid / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity*
chemical_list: D000588:Amines; D005520:Food Preservatives; D009153:Mutagens; D004247:DNA; D013011:Sorbic Acid