title: Equal induction and persistence of chromosome aberrations involving chromosomes 1, 4 and 10 in thyroid cancer patients treated with radioactive iodine.
abstract: A number of in vitro studies have questioned the assumption of random distribution of breaks in radiation-induced chromosome aberrations. The therapeutic application of radioactive 131I in thyroid cancer patients offers a good opportunity to study the induction and persistence of cytogenetic damage involving different chromosomes in vivo. Using whole-chromosome painting probes and triple colour painting by fluorescence in situ hybridization (FISH), we have analysed the frequency of chromosomal aberrations (CAs) involving chromosomes 1, 4 and 10 in peripheral blood lymphocytes of 10 thyroid cancer patients sampled before and 1 week, 1 year and 3.5 years after therapeutic application of radioactive iodine in a self-controlled, longitudinal study. A highly significant 3.4-fold increase in the frequency of chromosome breaks was observed 1 week after treatment with a similar representation of all chromosomes analysed. Although a significant decrease in dicentrics was observed during the first year after treatment, the frequency of chromosome aberrations remained over control levels until the last sampling time, 41-47 months post-treatment. The same behaviour, in terms of induction and persistence, was observed for all three chromosomes, confirming our previous results in vitro and rejecting the reported suggestion that chromosome 10 is radiosensitive in vivo. Our finding that the dynamics of radiation-induced CA in vivo is independent on the chromosome of choice suggests that this variable is not important in retrospective studies.
authors.affiliation: Grup de Mutagènesi, Unitat de Genet., Departament de Genètica i de Microbiologia, Edifici Cn, Universitat Autònoma de Barcelona, 08193, Bellaterra (Cerdanyola des Valles), Spain.
authors.forename: S
authors.initials: S
authors.lastname: Puerto
authors.forename: R
authors.initials: R
authors.lastname: Marcos
authors.forename: M J
authors.initials: MJ
authors.lastname: Ramírez
authors.forename: P
authors.initials: P
authors.lastname: Galofré
authors.forename: A
authors.initials: A
authors.lastname: Creus
authors.forename: J
authors.initials: J
authors.lastname: Surrallés
mesh_terms: D000328:Adult; D002648:Child; D002869:Chromosome Aberrations; D002874:Chromosome Mapping; D020223:Chromosome Painting / Q000379:methods; D002878:Chromosomes, Human, Pair 1; D002879:Chromosomes, Human, Pair 10; D002894:Chromosomes, Human, Pair 4; D005500:Follow-Up Studies; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007457:Iodine Radioisotopes / Q000627:therapeutic use*; D008214:Lymphocytes / Q000528:radiation effects*; D008875:Middle Aged; D011879:Radiotherapy Dosage; D013964:Thyroid Neoplasms / Q000097:blood / Q000532:radiotherapy*; D013997:Time Factors
chemical_list: D007457:Iodine Radioisotopes