Genes involved in the destruction of leukaemic cells by induced photosensitivity.
Gene expression changes were observed in the HEL and HL-60 cell lines after the stimulation of protoporphyrin IX synthesis by ALA administration and photodynamic process induction. Isolated ribonucleic acids were radiolabelled by reverse transcription, and the cDNA obtained was hybridized to membrane macroarrays (Clontech 7742-1) containing 588 gene probes. Besides changes in the activity of genes supposed to be involved in the programmed cell death and DNA reparation processes, increased or diminished transcription activity was also observed in several other genes; the reason for this phenomenon was not clear. The activation of programmed-cell-death genes appeared after the ALA load application, indicating the toxic effect of ALA. The gene expression changes observed in the two cell lines differed substantially, only a few of them were common for both cell lines.
Belicková
M
M
Institute of Haematology and Blood Transfusion, Prague, Czech Republic.
Bruchová
H
H
Cajthamlová
H
H
Hrkal
Z
Z
Brdicka
R
R
D000622:Aminolevulinic Acid / Q000494:pharmacology*; D017209:Apoptosis / Q000235:genetics*; D002470:Cell Survival; D018076:DNA, Complementary; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D018922:HL-60 Cells; D006801:Humans; D007938:Leukemia / Q000235:genetics* / Q000473:pathology; D004915:Leukemia, Erythroblastic, Acute; D008027:Light; D020411:Oligonucleotide Array Sequence Analysis; D017319:Photosensitizing Agents / Q000378:metabolism / Q000494:pharmacology*; D011524:Protoporphyrins / Q000096:biosynthesis* / Q000235:genetics; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D017319:Photosensitizing Agents; D011524:Protoporphyrins; D000622:Aminolevulinic Acid; C028025:protoporphyrin IX