title: External anions and volume-sensitive anion current in guinea-pig ventricular myocytes.
abstract: The objective of this study was to determine the effects of anion replacement on volume-sensitive anion current in guinea-pig ventricular myocytes. Myocytes in the conventional whole-cell voltage-clamp configuration were superfused and dialysed with Na(+)-, K(+)-, and Ca(2+)-free solution, and exposed to external 75 mM Cl- solution of one-half normal osmolality. Prolonged exposures to hyposmotic solution promoted the development of outwardly-rectifying currents that were inactivated at high positive potentials and reversed in a Cl(-)-dependent manner (50 mV per decade pipette Cl- concentration). Replacement of external Cl- by iodide and aspartate affected the reversal potential (E(rev)) and slope conductance of the volume-sensitive current. Relative permeabilities calculated from changes in E(rev) were 1.49 +/- 0.09, 1.00, and 0.29 +/- 0.04 for iodide, Cl-, and aspartate, respectively; relative slope conductances between E(rev) and E(rev) + 40 mV were 1.21 +/- 0.09, 1.00, and 0.43 +/- 0.07, respectively. Replacement of Cl- also affected the time dependence of the volume-sensitive current; replacement by iodide reversibly enhanced the decay of outward current at positive potentials, whereas replacement by aspartate reduced it. These results are compared with earlier findings on noncardiac time- and voltage-dependent anion current activated by hyposmotic solution.
authors.affiliation: Department of Physiology and Biophysics, Dalhousie University, Halifax, NS, Canada.
authors.forename: L M
authors.initials: LM
authors.lastname: Shuba
authors.forename: T F
authors.initials: TF
authors.lastname: McDonald
mesh_terms: D000818:Animals; D001224:Aspartic Acid / Q000494:pharmacology; D018118:Chloride Channels / Q000502:physiology; D002712:Chlorides / Q000378:metabolism*; D006168:Guinea Pigs; D006352:Heart Ventricles; D007454:Iodides / Q000494:pharmacology; D015640:Ion Channel Gating; D009206:Myocardium / Q000378:metabolism*; D010539:Permeability
chemical_list: D018118:Chloride Channels; D002712:Chlorides; D007454:Iodides; D001224:Aspartic Acid