Towards the column bed stabilization of columns in capillary electroendosmotic chromatography. Immobilization of microparticulate silica columns to a continuous bed.
This article discusses a novel method generating a continuous bed inside the CEC column. The column bed composed of microparticulate reversed-phase silica is completely immobilized by a hydrothermal treatment using water for the immobilization process. This process eliminates the manufacture of frits of both ends of the column and all problems associated with their preparation. Fundamental studies on operational parameters will be presented such as the dependence of the immobilization on the column temperature, the type of stationary phase and the column back pressure. The immobilized CEC columns show the same high column efficiency as packed columns with frits.
Adam
T
T
Institut für Anorganische Chemie und Analytische Chemie, Johannes Gutenberg-Universität, Mainz, Germany. thomas.adam.ta@bayer-ag.de
Unger
K K
KK
Dittmann
M M
MM
Rozing
G P
GP
D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D010316:Particle Size; D012822:Silicon Dioxide / Q000737:chemistry*; D013696:Temperature
D012822:Silicon Dioxide