Identification of the products of the equine herpesvirus type 4 gI and gE genes.
In order to identify the products of the equine herpesvirus type 4 (EHV-4) gI and gE genes, we have constructed recombinant vaccinia viruses containing the putative gI or gE genes. These recombinant viruses synthesized EHV-4 gI and gE with apparent molecular masses of 75 and 80kDa, respectively. Antibodies raised against both recombinant viruses detected a 75 kDa gI and a 95 kDa gE in EHV-4-infected cells. The results also suggest that the EHV-4 gI and gE would form a complex like in other herpesviruses.
Damiani
A M
AM
Department of Veterinary Microbiology, Faculty of Agriculture, The University of Tokyo, Japan.
Matsumura
T
T
Jang
H K
HK
Izumiya
Y
Y
Mikami
T
T
Takahashi
E
E
D000818:Animals; D005455:Fluorescent Antibody Technique; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D018140:Varicellovirus / Q000235:genetics* / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000235:genetics*
D014759:Viral Envelope Proteins; C111845:glycoprotein I, Equine herpesvirus 4; C111846:glycoprotein e, Equid herpesvirus 4