Hepatitis C virus core mutations reduce the sensitivity of a fluorescence enzyme immunoassay.
Four of 107 samples obtained from hepatitis C virus (HCV) carriers showed lower HCV core antigen levels in a fluorescence enzyme immunoassay (FEIA) than expected from corresponding HCV RNA levels. Nucleotide sequencing revealed a mutation in the HCV core region (Thr49Pro) that appears to have reduced the FEIA sensitivity.
Tokita
H
H
Department of Gastroenterology, National Tokyo Hospital, Kiyose-Shi, Tokyo 204-0023, Japan.
Kaufmann
G R
GR
Matsubayashi
M
M
Okuda
I
I
Tanaka
T
T
Harada
H
H
Mukaide
M
M
Suzuki
K
K
Cooper
D A
DA
D000595:Amino Acid Sequence; D005453:Fluorescence; D016174:Hepacivirus / Q000235:genetics / Q000302:isolation & purification*; D018936:Hepatitis C Antigens / Q000097:blood / Q000737:chemistry / Q000235:genetics*; D019698:Hepatitis C, Chronic / Q000175:diagnosis*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D008969:Molecular Sequence Data; D009154:Mutation; D012367:RNA, Viral / Q000097:blood; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D014758:Viral Core Proteins / Q000097:blood / Q000737:chemistry / Q000235:genetics*
D018936:Hepatitis C Antigens; D012367:RNA, Viral; D014758:Viral Core Proteins; C069130:nucleocapsid protein, Hepatitis C virus