Muscarinic M3 receptor subtype gene expression in the human heart.
The heart is an important target organ for cholinergic function. In this study, muscarinic receptor subtype(s) in the human heart were determined using reverse transcription-polymerase chain reaction. Our results demonstrated muscarinic receptor M2 and M3 subtype RNA in left/right atria/ventricles of donor hearts. Receptor autoradiography analysis using selective muscarinic ligands indicated an absence of M1 receptor subtype in the human heart. The level of muscarinic receptor binding in atria was two to three times greater than in ventricles. Our results suggest that muscarinic receptors in the human heart are of the M2 and M3 subtypes. This is the first report of M3 receptors in the human myocardium.
Hellgren
I
I
Department of Cardiology, NOVUM, Karolinska Institute, Huddinge University Hospital, Sweden. irina.hellgren@medhs.ki.se
Mustafa
A
A
Riazi
M
M
Suliman
I
I
Sylvén
C
C
Adem
A
A
D000293:Adolescent; D000328:Adult; D001345:Autoradiography; D002311:Cardiomyopathy, Dilated / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D006325:Heart Atria / Q000378:metabolism; D006352:Heart Ventricles / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008024:Ligands; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D043587:Receptor, Muscarinic M3; D011976:Receptors, Muscarinic / Q000032:analysis / Q000145:classification* / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013379:Substrate Specificity
D008024:Ligands; D012333:RNA, Messenger; D043587:Receptor, Muscarinic M3; D011976:Receptors, Muscarinic