title: Equine herpesvirus 1 (EHV-1) glycoprotein D DNA inoculation in horses with pre-existing EHV-1/EHV-4 antibody.
abstract: We have shown previously that equine herpesvirus 1 (EHV-1) glycoprotein D (gD) DNA elicited protective immune responses against EHV-1 challenge in murine respiratory and abortion models of EHV-1 disease. In this study, 20 horses, all with pre-existing antibody to EHV-4 and two with pre-existing antibody to EHV-1, were inoculated intramuscularly with three doses each of 50, 200 or 500microg EHV-1 gD DNA or with 500microg vector DNA. In 8 of 15 horses, inoculation with EHV-1 gD DNA led to elevated gD-specific antibody and nine horses exhibited increased virus neutralising (VN) antibody titres compared to those present when first inoculated. A lack of increase in gC-specific antibody during the 66 weeks of the experiment showed that the increase in gD-specific antibodies was not due to a natural infection with either EHV-1 or EHV-4. The increase in EHV-1 gD-specific antibodies was predominantly an IgGa and IgGb antibody response, similar to the isotype profile reported following natural EHV-1 infection.
authors.affiliation: Department of Biological Sciences, Division of Environmental and Life Sciences, Macquarie University, NSW 2109, Sydney, Australia.
authors.forename: K M
authors.initials: KM
authors.lastname: Ruitenberg
authors.forename: D N
authors.initials: DN
authors.lastname: Love
authors.forename: J R
authors.initials: JR
authors.lastname: Gilkerson
authors.forename: J E
authors.initials: JE
authors.lastname: Wellington
authors.forename: J M
authors.initials: JM
authors.lastname: Whalley
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D015153:Blotting, Western / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006389:Hemagglutinins, Viral / Q000276:immunology*; D006566:Herpesviridae Infections / Q000517:prevention & control / Q000662:veterinary*; D004861:Herpesvirus 1, Equid / Q000276:immunology*; D023141:Herpesvirus 4, Equid / Q000276:immunology*; D006734:Horse Diseases / Q000517:prevention & control*; D006736:Horses; D009500:Neutralization Tests / Q000662:veterinary; D014611:Vaccination / Q000662:veterinary*; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D006389:Hemagglutinins, Viral; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; C488988:glycoprotein D, Equid herpesvirus 1