title: Polysialic acid regulates chain formation by migrating olfactory interneuron precursors.
abstract: Olfactory interneuron precursors in the rostral migration stream migrate in chains and through long distances to the olfactory bulb. The migration is inhibited when polysialic acid moiety of NCAM is removed. How polysialic acid regulates chain migration has remained unknown. Previous studies in other systems have indicated the polysialic acid as a negative regulator of cell-cell interactions. Thus, polysialic acid may prevent cells in chains from interacting too tightly. To test this hypothesis and examine how polysialic acid regulates chain migration, the effect of polysialic acid depletion was evaluated in vitro and in vivo. Surprisingly, removal of polysialic acid often resulted in the dispersion of chains into single cells in both subventricular zone cultures and in adult mice where chain migration was observed. These results indicate that polysialic acid plays an important role in the formation of chains by olfactory interneuron precursors.
authors.affiliation: Rammelkamp Center for Education and Research, MetroHealth Medical Center, Cleveland, Ohio, USA. hhu@research.metrohealth.org
authors.forename: H
authors.initials: H
authors.lastname: Hu
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D007395:Interneurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D007797:Laminin / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D019006:Neural Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism; D009830:Olfactory Bulb / Q000166:cytology* / Q000196:embryology / Q000378:metabolism*; D011509:Proteoglycans / Q000494:pharmacology; D012794:Sialic Acids / Q000378:metabolism* / Q000494:pharmacology; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects
chemical_list: D004338:Drug Combinations; D007797:Laminin; D019006:Neural Cell Adhesion Molecules; D011509:Proteoglycans; D012794:Sialic Acids; C021319:polysialic acid; C059485:matrigel; D003094:Collagen