Fe(III) reduction activity and cytochrome content of Shewanella putrefaciens grown on ten compounds as sole terminal electron acceptor.
Shewanella putrefaciens was grown on a series of ten alternate compounds as sole terminal electron acceptor. Each cell type was analyzed for Fe(III) reduction activity, absorbance maxima in reduced-minus-oxidized difference spectra and heme-containing protein content. High-rate Fe(III) reduction activity, pronounced difference maxima at 521 and 551 nm and a predominant 29.3 kDa heme-containing protein expressed by cells grown on Fe(III), Mn(IV), U(VI), SO3(2-) and S2O3(2-), but not by cells grown on O2, NO3, NO2-, TMAO or fumarate. These results suggest that microbial Fe(III) reduction activity is enhanced by anaerobic growth on metals and sulfur compounds, yet is limited under all other terminal electron-accepting conditions.
Blakeney
M D
MD
School of Biology, Georgia Institute of Technology, Atlanta 30332-0230, USA.
Moulaei
T
T
DiChristina
T J
TJ
D003580:Cytochromes / Q000032:analysis / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005290:Ferric Compounds / Q000378:metabolism*; D006420:Hemeproteins / Q000032:analysis / Q000378:metabolism; D010084:Oxidation-Reduction; D020614:Shewanella putrefaciens / Q000254:growth & development / Q000378:metabolism*; D013053:Spectrophotometry
D003580:Cytochromes; D005290:Ferric Compounds; D006420:Hemeproteins