title: [Optimal conditions for establishment of experimental tuberculosis model using an automated inhalation exposure apparatus and its application].
abstract: Animal (mouse and guinea pig) pulmonary tuberculosis models were established, using an automated inhalation exposure apparatus (Glas-Col Corp., USA, Model 099CA-4212). This apparatus includes four steps--preheating, nebulization, cloud decay and decontamination. The optimal conditions for M. tuberculosis H37Rv strain infection experiments were as follows: 10(5-6) colony forming unit (cfu) tubercle bacilli; preheating for 15 min.; nebulization for 90 min.; cloud decay for 15 min. and decontamination for 5 min. When 10(4) cfu M. tuberculosis H37Rv strain were introduced into the lungs of interferon (IFN)-gamma knockout mice, using the inhalation exposure apparatus and were followed up for 9 months, the primitive cavitary lesions were observed. This apparatus was also useful for inhalation exposure experiments of guinea pigs. This apparatus can also be utilized for animal inhalation experiments of allergens.
authors.affiliation: Department of Molecular Pathology, Japan Anti-tuberculosis Association, Tokyo, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Sugawara
authors.forename: H
authors.initials: H
authors.lastname: Yamada
authors.forename: K
authors.initials: K
authors.lastname: Otomo
authors.forename: T
authors.initials: T
authors.lastname: Aoki
authors.forename: S
authors.initials: S
authors.lastname: Mizuno
authors.forename: T
authors.initials: T
authors.lastname: Udagawa
mesh_terms: D000818:Animals; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D005260:Female; D006168:Guinea Pigs; D019570:Inhalation Exposure; D007371:Interferon-gamma; D051379:Mice; D018345:Mice, Knockout; D009169:Mycobacterium tuberculosis; D009330:Nebulizers and Vaporizers; D014397:Tuberculosis, Pulmonary / Q000382:microbiology / Q000473:pathology
chemical_list: D007371:Interferon-gamma