title: Act1, an NF-kappa B-activating protein.
abstract: Use of an NF-kappaB-dependent selectable marker facilitated the isolation of a cell line containing a cDNA encoding Act1, an NF-kappaB activator. Act1 associates with and activates IkappaB kinase (IKK), leading to the liberation of NF-kappaB from its complex with IkappaB. Many signaling pathways that liberate NF-kappaB also activate activating transcription factor (ATF) and activator protein 1 (AP-1) through Jun kinase (JNK). Act1 also activates JNK, suggesting that it might be part of a multifunctional complex involved in the activation of both NF-kappaB and JNK. Act1 fails to activate NF-kappaB in an IL-1-unresponsive mutant cell line in which all known signaling components are present, suggesting that it interacts with an unknown component in IL-1 signaling.
authors.affiliation: Departments of Immunology and Molecular Biology, Lerner Research Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, OH 44195, USA.
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: M
authors.initials: M
authors.lastname: Commane
authors.forename: H
authors.initials: H
authors.lastname: Nie
authors.forename: X
authors.initials: X
authors.lastname: Hua
authors.forename: M
authors.initials: M
authors.lastname: Chatterjee-Kishore
authors.forename: D
authors.initials: D
authors.lastname: Wald
authors.forename: M
authors.initials: M
authors.lastname: Haag
authors.forename: G R
authors.initials: GR
authors.lastname: Stark
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D002352:Carrier Proteins; D002460:Cell Line; D018076:DNA, Complementary; D004789:Enzyme Activation; D018257:Helix-Loop-Helix Motifs; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism; D048031:JNK Mitogen-Activated Protein Kinases; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D047988:Tumor Necrosis Factor Receptor-Associated Peptides and Proteins
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D018076:DNA, Complementary; D007375:Interleukin-1; D016328:NF-kappa B; D011994:Recombinant Proteins; C414202:TRAF3IP2 protein, human; D015534:Trans-Activators; D047988:Tumor Necrosis Factor Receptor-Associated Peptides and Proteins; D048031:JNK Mitogen-Activated Protein Kinases; D020928:Mitogen-Activated Protein Kinases