title: Expression of the tetA(C) tetracycline efflux pump in Escherichia coli confers osmotic sensitivity.
abstract: Expression of tetA(C) in Escherichia coli confers resistance to tetracycline as well as sensitivity to nickel and cadmium salts, lipophilic chelating agents, and aminoglycoside antibiotics. In this report we determine that high-level expression of tetA(C) also confers an osmotic sensitivity. The osmotic-sensitive phenotype is distinct from the tetracycline-resistant phenotype and can be localized to a domain contained within the first 98 amino acid residues of the TetA(C) polypeptide.
authors.affiliation: The John P. Robarts Research Institute, London, Ont., Canada.
authors.forename: T A
authors.initials: TA
authors.lastname: Stavropoulos
authors.forename: C A
authors.initials: CA
authors.lastname: Strathdee
mesh_terms: D017920:Antiporters / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002463:Cell Membrane Permeability; D003470:Culture Media / Q000737:chemistry; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000502:physiology*; D009532:Nickel / Q000494:pharmacology; D009997:Osmotic Pressure; D010641:Phenotype; D017434:Protein Structure, Tertiary; D013753:Tetracycline Resistance / Q000235:genetics
chemical_list: D017920:Antiporters; D001426:Bacterial Proteins; D003470:Culture Media; C079051:tetA protein, Bacteria; D009532:Nickel