title: Synthesis of 60- and 72 kDa heat shock proteins in early porcine embryogenesis.
abstract: Proteins of selected embryonic stages were metabolically labeled with [(35)S]-methionine and analyzed by two-dimensional SDS-polyacrylamide gel electrophoresis (2-D PAGE) to study protein expression from 4- to 8-cell to blastocyst stage of porcine embryos. Two proteins with molecular weights of 60 and 72kDa were de novo synthesized during the 4- to 8-cell stage were the earliest that were detected. They were identified as HSP60 and HSP72 according to their locations on 2-D autoradiography and the immunoblotting result of anti-HSP 60 and HSP 72 antibodies of 1-cell stage of porcine embryos. In protein translation in early pig embryogenesis the timing of their synthesis suggests that HSP60 and HSP72 play significant roles as chaperones.
authors.affiliation: Department of Comparative Medicine, Pig Research Institute Taiwan, P. O. Box 23, Chunan, 35099, ROC, Miaoli, Taiwan.
authors.forename: Y T
authors.initials: YT
authors.lastname: King
authors.forename: W C
authors.initials: WC
authors.lastname: Lee
authors.forename: M S
authors.initials: MS
authors.lastname: Gao
authors.forename: J L
authors.initials: JL
authors.lastname: Wang
authors.forename: C F
authors.initials: CF
authors.lastname: Tu
authors.forename: S C
authors.initials: SC
authors.lastname: Wu
authors.forename: Y H
authors.initials: YH
authors.lastname: Kuo
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D018834:Chaperonin 60 / Q000096:biosynthesis*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000662:veterinary; D005260:Female; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D007315:Insemination, Artificial / Q000662:veterinary; D008297:Male; D008970:Molecular Weight; D013552:Swine / Q000196:embryology*
chemical_list: D018834:Chaperonin 60; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins