Induction of membrane chloride currents in Xenopus laevis oocytes by the sulfonyl amide sweeteners acesulfame K and saccharin.
Sweet receptors have remained elusive. In Xenopus oocytes sulfonyl amide sweeteners but not sweet compounds belonging to other chemical classes dose dependently induced membrane chloride currents via the inositol trisphosphate/calcium pathway. Induction of membrane currents was exclusively observed following extracellular application of sulfonyl amides but not by intracellular pressure injection, suggesting the involvement of a plasma membrane receptor. The presence of this receptor in oocytes and the observed seasonal variation of the sweet response offers an opportunity for a molecular cloning approach.
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Deutsches Institut für Ernährungsforschung, Abteilung Molekulare Genetik und Universität Potsdam, Potsdam-Rehbrücke, Germany.
Schmale
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Meyerhof
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D000818:Animals; D018118:Chloride Channels / Q000187:drug effects*; D005260:Female; D009865:Oocytes / Q000187:drug effects* / Q000378:metabolism; D012439:Saccharin / Q000494:pharmacology*; D013549:Sweetening Agents / Q000494:pharmacology*; D013843:Thiazines / Q000494:pharmacology*; D014982:Xenopus laevis
D018118:Chloride Channels; D013549:Sweetening Agents; D013843:Thiazines; D012439:Saccharin; C006362:acetosulfame