title: Diarylheptanoids with in vitro inducible nitric oxide synthesis inhibitory activity from Alnus hirsuta.
abstract: Four diarylheptanoids were isolated from the leaf of Alnus hirsuta (Betulaceae) and have been assessed for nitric oxide (NO) production inhibitory effects in vitro. Oregonin (1) and hirsutanonol (2) were found to be potent inducible nitric oxide synthase (iNOS) inhibitors. Compounds 1 and 2 showed inhibition of NO synthesis in dose-dependent manners by murine macrophage-like RAW 264.7 cells stimulated with interferon-gamma (IFN-gamma) plus lipopolysaccharide (LPS). Their 50% inhibitory concentrations (IC50) were 3.8 and 14.3 microM, respectively. The inhibitory effects of these compounds on NO synthesis were due to suppression of iNOS mRNA expression as determined by Northern blotting.
authors.forename: M W
authors.initials: MW
authors.lastname: Lee
authors.forename: N Y
authors.initials: NY
authors.lastname: Kim
authors.forename: M S
authors.initials: MS
authors.lastname: Park
authors.forename: K H
authors.initials: KH
authors.lastname: Ahn
authors.forename: S H
authors.initials: SH
authors.lastname: Toh
authors.forename: D R
authors.initials: DR
authors.lastname: Hahn
authors.forename: Y C
authors.initials: YC
authors.lastname: Kim
authors.forename: H T
authors.initials: HT
authors.lastname: Chung
mesh_terms: D000818:Animals; D002460:Cell Line; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006536:Heptanes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D051379:Mice; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D052247:Nitric Oxide Synthase Type II; D010944:Plants / Q000737:chemistry*
chemical_list: D004791:Enzyme Inhibitors; D006536:Heptanes; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse