title: Mutations in a tRNA import signal define distinct receptors at the two membranes of Leishmania mitochondria.
abstract: Nucleus-encoded tRNAs are selectively imported into the mitochondrion of Leishmania, a kinetoplastid protozoan. An oligoribonucleotide constituting the D stem-loop import signal of tRNA(Tyr)(GUA) was efficiently transported into the mitochondrial matrix in organello as well as in vivo. Transfer through the inner membrane could be uncoupled from that through the outer membrane and was resistant to antibody against the outer membrane receptor TAB. A number of mutations in the import signal had differential effects on outer and inner membrane transfer. Some mutants which efficiently traversed the outer membrane were unable to enter the matrix. Conversely, restoration of the loop-closing GC pair in reverse resulted in reversion of transfer through the inner, but not the outer, membrane, and binding of the RNA to the inner membrane was restored. These experiments indicate the presence at the two membranes of receptors with distinct specificities which mediate stepwise transfer into the mitochondrial matrix. The combination of oligonucleotide mutagenesis and biochemical fractionation may provide a general tool for the identification of tRNA transport factors.
authors.affiliation: Genetic Engineering Laboratory, Indian Institute of Chemical Biology, Calcutta 700032, India.
authors.forename: S N
authors.initials: SN
authors.lastname: Bhattacharyya
authors.forename: S
authors.initials: S
authors.lastname: Mukherjee
authors.forename: S
authors.initials: S
authors.lastname: Adhya
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D020029:Base Pairing; D001693:Biological Transport, Active; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D002414:Cations, Monovalent / Q000494:pharmacology; D007425:Intracellular Membranes / Q000378:metabolism*; D007895:Leishmania tropica / Q000235:genetics* / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D008954:Models, Biological; D016297:Mutagenesis, Site-Directed; D009550:Nigericin / Q000494:pharmacology; D009690:Nucleic Acid Conformation; D010641:Phenotype; D017354:Point Mutation; D011485:Protein Binding; D015800:Protozoan Proteins / Q000378:metabolism*; D016053:RNA, Protozoan / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012365:RNA, Transfer, Tyr / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D013329:Structure-Activity Relationship; D013696:Temperature; D014475:Uncoupling Agents / Q000494:pharmacology
chemical_list: D002414:Cations, Monovalent; D008565:Membrane Proteins; D015800:Protozoan Proteins; D016053:RNA, Protozoan; D012365:RNA, Transfer, Tyr; D016601:RNA-Binding Proteins; C107685:TAB protein, Leishmania donovani; D014475:Uncoupling Agents; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D000255:Adenosine Triphosphate; D009550:Nigericin