title: Preliminary EEG study of protective effects of Tebonin in transient global cerebral ischemia in rats.
abstract: Free radicals and platelet activating factor (PAF) have been implicated as important mediators in neuronal injury after cerebral ischemia-reperfusion and, particularly, in postischemic hypoperfusion. The electroencephalography (EEG) is a real time reflection of changes in neuronal activity and metabolism. The objective of this study was to investigate the effects of preventive treatment with Ginkgo biloba extract (EGb 761--Tebonin) in cerebral global ischemia and reperfusion in rats using computerized EEG analysis. Ginkgo biloba extract, known to be, in vitro, a free radicals scavanger and a PAF--antagonist, was administrated in dose of 100 mg/kg over 24 hours, for 5 days before and 5 days after cerebral ischemia--reperfusion. The apparition of isoelectric EEG (flat-line) following 4-vessel occlusion was observed after a mean time of 25 sec. in Ginkgo biloba treated rats and after 18 sec. in control rats (p < 0.0015). Computerized spectral analysis of EEG has shown that the percentage of slow waves at 10 minutes after reperfusion was 117% higher in control group than in Ginkgo biloba group (p < 0.015) and the percentage of slow waves after 15 minutes of reperfusion was 100% higher in the control group than in the Ginkgo biloba group (p < 0.02). Five days after cerebral ischemia--reperfusion the percentage of slow waves was unsignificantly higher in the control group than in the Ginkgo biloba group (p > 0.05).
authors.affiliation: Department of Physiology, N. Gh. Lupu Hospital, Bucharest.
authors.forename: L
authors.initials: L
authors.lastname: Zăgrean
authors.forename: R
authors.initials: R
authors.lastname: Vătăşescu
authors.forename: A M
authors.initials: AM
authors.lastname: Munteanu
authors.forename: M
authors.initials: M
authors.lastname: Moldovan
authors.forename: D A
authors.initials: DA
authors.lastname: Niţă
authors.forename: M
authors.initials: M
authors.lastname: Coculescu
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000503:physiopathology*; D004569:Electroencephalography; D005260:Female; D005419:Flavonoids / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D020441:Ginkgo biloba; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010936:Plant Extracts; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors*; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D015427:Reperfusion Injury / Q000503:physiopathology; D000096983:Ginkgo Extract
chemical_list: D005419:Flavonoids; D016166:Free Radical Scavengers; D018696:Neuroprotective Agents; D010936:Plant Extracts; D010972:Platelet Activating Factor; C063170:Ginkgo biloba extract; D000096983:Ginkgo Extract