Lymphoid/neuronal cell surface OX2 glycoprotein recognizes a novel receptor on macrophages implicated in the control of their function.
The OX2 membrane glycoprotein (CD200) is expressed on a broad range of tissues including lymphoid cells, neurons, and endothelium. We report the characterization of an OX2 receptor (OX2R) that is a novel protein restricted to cells of the myeloid lineage. OX2 and its receptor are both cell surface glycoproteins containing two immunoglobulin-like domains and interact with a dissociation constant of 2.5 microM and koff 0.8 s(-1), typical of many leukocyte protein membrane interactions. Pervanandate treatment of macrophages showed that OX2R could be phosphorylated on tyrosine residues. Blockade of the OX2-OX2R interaction with an OX2R mAb exacerbated the disease model experimental allergic encephalomyelitis. These data, together with data from an OX2-deficient mouse (R. M. Hoek et al., submitted), suggest that myeloid function can be controlled in a tissue-specific manner by the OX2-OX2R interaction.
Wright
G J
GJ
Sir William Dunn School of Pathology, University of Oxford, United Kingdom.
Puklavec
M J
MJ
Willis
A C
AC
Hoek
R M
RM
Sedgwick
J D
JD
Brown
M H
MH
Barclay
A N
AN
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D007136:Immunoglobulins / Q000502:physiology; D019891:Leukopoiesis / Q000502:physiology*; D008214:Lymphocytes / Q000502:physiology; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D008969:Molecular Sequence Data; D009474:Neurons / Q000502:physiology; D051381:Rats; D011971:Receptors, Immunologic / Q000502:physiology*; D016415:Sequence Alignment; D015398:Signal Transduction / Q000502:physiology
D007136:Immunoglobulins; D011971:Receptors, Immunologic