Simultaneous determination of fat-soluble vitamins and provitamins in milk by microcolumn liquid chromatography.
The fat-soluble vitamins A, D2, D3, E and K1, retinyl acetate, retinyl palmitate, tocopherol acetate, ergosterol and 7-dehydrocholesterol in milk were separated on a packed reversed-phase fused-silica microcolumn, and determined with UV detection. Conventional liquid chromatographic equipment was adapted for such purposes, providing a highly efficient and sensitive analytical system. The vitamins could be separated in gradient mode with detection limits comprised between 0.02 ng/ml for retinol and 2 ng/ml for vitamin E. All vitamins were separated in less than 17 min. Recovery studies showed good results for all solutes (89-107%) and the intra-day coefficients of variations ranged from 2 to 8%. Application of the proposed method to the quantification of fat-soluble vitamins in milk is reported.
Gomis
D B
DB
Departamento de Quimica Física y Analítica, Universidad de Oviedo, Spain. dbg@sauron.quimica.uniovi.es
Fernández
M P
MP
Gutiérrez Alvarez
M D
MD
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008892:Milk / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D014815:Vitamins / Q000032:analysis*
D014815:Vitamins