title: Auditory threshold and inner ear pressure: measurements in experimental endolymphatic hydrops.
abstract: OBJECTIVE
  Experimental endolymphatic hydrops leads to an endolymph-perilymph pressure imbalance that is responsible for the loss of auditory sensitivity in the ear.
  
  
  BACKGROUND
  This study investigates whether intralabyrinthine pressure is a factor in the auditory dysfunction of experimental endolymphatic hydrops.
  
  
  METHODS
  Auditory function was investigated in 10 guinea pigs 90 to 120 days after endolymphatic sac ablation by measuring compound action potentials in response to acoustic stimuli including alternating clicks and tone bursts of 0.5, 1, 2, 4, and 8 kHz. After auditory thresholds to the various stimuli were established, endolymphatic and perilymphatic pressures were measured with a no-flow micropressure measuring system. The cause of the hearing loss in Meniere's disease, especially in the early phases of this condition, remains an enigma. Histologic temporal bone preparations show a disproportion in the volume of endolymph to perilymph with an expanded and distorted membranous labyrinth. Speculation as to whether an endolymph-perilymph fluid pressure imbalance is responsible for these morphologic changes were raised by Hallpike and Cairns (1) in their initial report of the histopathology of Meniere's disease. Further speculation has questioned whether this pressure imbalance may be responsible for the symptoms and physical finding of this condition, including hearing loss. The ability to produce experimental endolymphatic hydrops in the guinea pig secondary to surgical occlusion of the endolymphatic sac and duct has been useful in studying various aspects of Meniere's disease (2). Investigators have been able to demonstrate auditory threshold shifts with hydrops. Elevated endolymph-perilymph pressure gradients have also been demonstrated (3). This
  
  
  RESULTS
  Increased auditory thresholds were noted in the hydrops ears at all tested parameters (p < 0.03). A relative increase in endolymph over perilymph pressure was found in hydrops ears as previously reported. The increase in endolymph-perilymph pressure of hydrops could not be correlated directly to the elevated auditory threshold shift.
  
  
  CONCLUSIONS
  Endolymphatic hydrops is a complex pathologic state with multiple inner ear alterations including abnormal intralabyrinthine pressure. Factors other than or in addition to pressure contribute to the auditory threshold shift of hydrops.
authors.affiliation: Division of Head and Neck Surgery, University of California Los Angeles Medical Center, Los Angeles 90095-1624, USA.
authors.forename: J C
authors.initials: JC
authors.lastname: Andrews
authors.forename: A
authors.initials: A
authors.lastname: Böhmer
authors.forename: L
authors.initials: L
authors.lastname: Hoffman
authors.forename: D
authors.initials: D
authors.lastname: Strelioff
mesh_terms: D000818:Animals; D001309:Auditory Threshold / Q000502:physiology*; D004195:Disease Models, Animal; D007758:Ear, Inner / Q000502:physiology*; D018159:Endolymphatic Hydrops / Q000175:diagnosis*; D006168:Guinea Pigs; D008365:Manometry / Q000379:methods*; D011312:Pressure