title: Identifying the promoter region of the human brain sodium channel subtype II gene (SCN2A).
abstract: Sodium channel genes are highly regulated. To begin analyzing the human brain sodium channel subtype II gene, SCN2A, at the transcriptional level, we mapped multiple transcriptional start sites within a 397 bp stretch of the 5'-UTR and -flanking region. When inserted into a basic luciferase reporter vector, this 397 bp region can promote luciferase expression in transiently transfected neuroblastoma cells, but not in non-neuronal cells. Thus, this study provides the initial description of a functional promoter in a human voltage-gated sodium channel gene.
authors.affiliation: Department of Psychiatry and Behavioral Neurobiology, Sparks Center, room 1075, University of Alabama at Birmingham, Birmingham, AL 35294-0017, USA. schade@uab.edu
authors.forename: S D
authors.initials: SD
authors.lastname: Schade
authors.forename: G B
authors.initials: GB
authors.lastname: Brown
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D017930:Genes, Reporter; D006801:Humans; D008156:Luciferases / Q000235:genetics; D008969:Molecular Sequence Data; D062551:NAV1.2 Voltage-Gated Sodium Channel; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009447:Neuroblastoma; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D015222:Sodium Channels / Q000235:genetics*; D016385:TATA Box; D014158:Transcription, Genetic; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D020121:5' Untranslated Regions; D062551:NAV1.2 Voltage-Gated Sodium Channel; D009419:Nerve Tissue Proteins; D011993:Recombinant Fusion Proteins; C568251:SCN2A protein, human; D015222:Sodium Channels; D008156:Luciferases