title: Bacterial dna sequences in prostate tissue from patients with prostate cancer and chronic prostatitis.
abstract: OBJECTIVE
  Although bacterial genetic material has been detected in prostate tissue from patients with various disorders, the prevalence of these organisms is unknown. We tested the hypothesis that bacterial detection rates differ between patients with prostate cancer and those with the chronic prostatitis/pelvic pain syndrome.
  
  
  METHODS
  Sterile prostate biopsies were obtained during radical retropubic prostatectomy from 107 patients with prostate cancer and using a perineal approach from 170 with the chronic prostatitis/pelvic pain syndrome. Numerous controls were also evaluated. Bacterial ribosomal encoding DNA (165 rDNA) sequences were detected using a polymerase chain reaction assay. Selected positives were cloned, sequenced and compared with DNA databases.
  
  
  RESULTS
  Bacterial DNA sequences were detected in 21 (19. 6%) of 107 patients with prostate cancer compared to 79 (46.4%) of 170 with chronic prostatitis (p <0.0001). These bacteria included urogenital pathogens, other described microorganisms and bacteria not reported previously.
  
  
  CONCLUSIONS
  Bacterial DNA sequences may be identified in prostate tissue from many patients. Bacterial detection rates in prostate tissue appear to differ among populations, with higher rates among patients with the chronic prostatitis/pelvic pain syndrome than among those with prostate cancer. Future studies of the role of various bacteria in the prostate may provide insight into the pathophysiology of prostate disease.
authors.affiliation: Department of Urology, University of Washington, School of Medicine, Seattle, Washington, USA.
authors.forename: J N
authors.initials: JN
authors.lastname: Krieger
authors.forename: D E
authors.initials: DE
authors.lastname: Riley
authors.forename: R L
authors.initials: RL
authors.lastname: Vesella
authors.forename: D C
authors.initials: DC
authors.lastname: Miner
authors.forename: S O
authors.initials: SO
authors.lastname: Ross
authors.forename: P H
authors.initials: PH
authors.lastname: Lange
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002908:Chronic Disease; D004269:DNA, Bacterial / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011471:Prostatic Neoplasms / Q000382:microbiology*; D011472:Prostatitis / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial