Identification of Brucella abortus OxyR and its role in control of catalase expression.
We report the cloning and sequencing of the Brucella abortus oxyR homolog and provide evidence that the transcription product of this gene binds to the B. abortus catalase promoter region. A gene replacement/deletion Brucella oxyR mutant exhibits increased sensitivity to prolonged exposure to H(2)O(2) and is unable to adapt to H(2)O(2) in the environment.
Kim
J A
JA
Department of Zoology and Genetics, Iowa State University, Ames, Iowa 50011, USA.
Mayfield
J
J
D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D001483:Base Sequence; D002003:Brucella abortus / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D002374:Catalase / Q000235:genetics*; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D015971:Gene Expression Regulation, Enzymologic; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; D006861:Hydrogen Peroxide; D002374:Catalase