Studies on the extracellular tannase from newly isolated Bacillus licheniformis KBR 6.
A tannase producing bacterial strain KBR 6 has been isolated from lateritic soil and identified as Bacillus licheniformis. It is capable of producing tannase in the medium containing only tannic acid. The rapid degradation of tannic acid and production of extracellular tannase was observed in three different media containing tannic acid (M1), tannic acid + basal salt (M2) and tannic acid + basal salt + glucose (M3). Maximum enzyme production and growth of the organism was obtained at 18-21 h and 30-36 h, respectively. The increased order of enzyme production in relation to different media is as per the following sequence, M3 > M2 > M1. The maximum growth and enzyme production was observed at pH 5.0. The pH and temperature optima of the enzyme activity were found to be at 5.75 and 60 degrees C respectively. Paper chromatographic analysis indicates that gallic acid is the enzymatic degradative product of tannic acid.
Mondal
K C
KC
Department of Botany and Forestry, Vidyasagar University, Midnapore, West Bengal, India.
Pati
B R
BR
D001407:Bacillus / Q000145:classification / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification; D002265:Carboxylic Ester Hydrolases / Q000096:biosynthesis*; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D047348:Hydrolyzable Tannins / Q000378:metabolism; D012988:Soil Microbiology; D013696:Temperature; D014197:Trees
D003470:Culture Media; D047348:Hydrolyzable Tannins; D002265:Carboxylic Ester Hydrolases; C022711:tannase