Mitochondrial metabolism impairment in muscle fibres of rats chronically intoxicated with Senna occidentalis seeds.
The chronic administration of S. occidentalis seeds was found to induce a mitochondrial myopathy in hens. This study was undertaken to determine if the chronic treatment with S. occidentalis seeds of rats (as a mammalian model) would induce a mitochondrial myopathy similar to those described in humans and to determine if the histological changes could be correlated with the amount of ingested seeds. Twenty-one days old rats were fed S. occidentalis seeds at different diet concentrations (1, 2, 3%). Rats fed 1% S. occidentalis seeds had only a few COX-negative muscle fibers in the pectoralis major muscle. Rats fed 3% Senna occidentalis seeds had a greater number of COX-negative fibers. Rats fed 2% had an intermediate number of COX-negative fibers. Activity of SDH and NADH-tr were decreased in rats of groups 2% and 3%. Our data indicate that a progressive mitochondrial metabolism impairment can be produced in rats fed S. occidentalis seeds and that this impairment can be correlated with the amount of ingested seeds.
Calore
E E
EE
Department of Pathology, Sao Paulo University Medical School, Brazil. calore@uol.com.br
Weg
R
R
Haraguchi
M
M
Calore
N M
NM
Cavaliere
M J
MJ
Sesso
A
A
D000284:Administration, Oral; D000818:Animals; D002366:Cassia / Q000737:chemistry*; D002400:Cathartics / Q000633:toxicity*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000502:physiology*; D017240:Mitochondrial Myopathies / Q000139:chemically induced* / Q000503:physiopathology; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000502:physiology; D010946:Plants, Medicinal; D051381:Rats; D012639:Seeds; D012676:Senna Extract / Q000633:toxicity*
D002400:Cathartics; D012676:Senna Extract