Ribonuclease T1 cleaves RNA after guanosines within single-stranded gaps of any length.
RNA-oligonucleotides with defined single-stranded stretches were designed to investigate the minimal requirements of a ribonuclease T1 substrate. It could be shown, that RNase T1 cleaves single-stranded RNA after a unique guanosine flanked by two double-stranded areas. However, the turnover of such a G-gap is significantly lower than that of a gap of two, three or four nucleotides.
Greiner-Stöffele
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Institut für Biochemie, Fakultät für Biowissenschaften, Pharmazie und Psychologie, Universität Leipzig, Germany.
Foerster
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HH
Hahn
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D001483:Base Sequence; D006151:Guanosine / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009711:Nucleotides / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012313:RNA / Q000378:metabolism*; D006163:Ribonuclease T1 / Q000378:metabolism*
D009711:Nucleotides; D006151:Guanosine; D012313:RNA; D006163:Ribonuclease T1