Periodontal repair in dogs: effect of transforming growth factor-beta 1 on alveolar bone and cementum regeneration.
BACKGROUND
Transforming growth factor-beta (TGF-beta) represents a family of growth-modulating proteins with fundamental roles in connective tissue and bone development. The objective of this study was to evaluate the potential for regeneration of alveolar bone and cementum following surgical implantation of recombinant human TGF-beta 1 (rhTGF-beta 1).


METHODS
Bilateral, critical size, supra-alveolar periodontal defects in 5 beagle dogs were surgically implanted with rhTGF-beta 1 in a calcium carbonate carrier (CaCO3) or with carrier alone. The animals were euthanized at 4 weeks postsurgery and block-biopsies of the defects were processed for histologic and histometric analysis.


RESULTS
Surgical implantation of rhTGF-beta 1 resulted in minimal, if any, stimulation of alveolar bone or cementum regeneration. Linear bone and cementum regeneration in rhTGF-beta 1-treated defects was 1.2+/-0.6 and 0.01+0.01 mm, respectively. Corresponding values for the controls were 1.0+/-0.6 and 0.01+/-0.03 mm.


CONCLUSIONS
The results suggest that, under the conditions (dose, carrier, defect type) evaluated here, treatment of periodontal defects in beagle dogs with rhTGF-beta 1 may be of limited clinical benefit.
Tatakis
D N
DN
Loma Linda University, Advanced Education Program in Periodontics, CA 92354, USA. dTatakis@sd.llu.edu
Wikesjö
U M
UM
Razi
S S
SS
Sigurdsson
T J
TJ
Lee
M B
MB
Nguyen
T
T
Ongpipattanakul
B
B
Hardwick
R
R
D000539:Alveolar Process / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology; D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects* / Q000502:physiology; D003739:Dental Cementum / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology; D004195:Disease Models, Animal; D004285:Dogs; D004353:Drug Evaluation, Preclinical; D004343:Drug Implants; D006801:Humans; D008297:Male; D010510:Periodontal Diseases / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D011994:Recombinant Proteins / Q000008:administration & dosage; D016212:Transforming Growth Factor beta / Q000008:administration & dosage*
D004343:Drug Implants; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta