Analysis of ORF 1 in European porcine reproductive and respiratory syndrome virus by long RT-PCR and restriction fragment length polymorphism (RFLP) analysis.
A rapid method was developed for partial characterization of the replicase-encoding open reading frame 1 (ORF 1) of porcine reproductive and respiratory syndrome virus (PRRSV). It comprised long RT-PCR amplification of 11.1 kb (94%) of ORF 1, followed by restriction fragment length polymorphism analysis. The method was used to compare ORF 1 sequences of two divergent European-type PRRSV strains. Our results indicated that the structural and replicase parts of these two strains had evolved at overall similar rates.
Nielsen
H S
HS
Danish Veterinary Institute for Virus Research, Ministry of Agriculture and Fisheries, Lindholm, DK-4771 Kalvehave, Denmark.
Storgaard
T
T
Oleksiewicz
M B
MB
D015139:Blotting, Southern / Q000662:veterinary; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D016366:Open Reading Frames; D012150:Polymorphism, Restriction Fragment Length; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics*; D012367:RNA, Viral / Q000737:chemistry; D012324:RNA-Dependent RNA Polymerase / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary*
D018076:DNA, Complementary; D012367:RNA, Viral; D012324:RNA-Dependent RNA Polymerase