The main cold shock protein of Listeria monocytogenes belongs to the family of ferritin-like proteins.
The transfer of the food-borne pathogen Listeria monocytogenes from 30 to 5 degrees C was characterized by the sharp induction of a low molecular mass protein. This major cold shock protein has an isoelectric point at pH 5.1 and a molecular mass of about 18 kDa, as observed on two-dimensional gel electrophoresis (2-DE) pattern. Its N-terminal sequence, obtained from the 2-DE spot, shared a complete sequence identity with a Listeria innocua non-heme iron-binding ferritin. The purification of these ferritin-like proteins (Flp) revealed a native molecular mass of about 100-110 kDa which indicates a polypeptide composed of six 18 kDa-subunits. Northern analysis indicated the presence of a 0.8-kb monocistronic mRNA in exponential growing cells and an important increase inflp mRNA amount after a downshift but also an upshift in temperature.
Hébraud
M
M
Unité de Recherches sur la Viande, Institut National de la Recherche Agronomique de Theix, Champanelle, France. hebraud@clermont.inra.fr
Guzzo
J
J
D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000145:classification / Q000235:genetics; D003080:Cold Temperature; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005293:Ferritins / Q000096:biosynthesis* / Q000737:chemistry / Q000145:classification / Q000235:genetics; D018869:Heat-Shock Response; D006801:Humans; D008089:Listeria monocytogenes / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D008969:Molecular Sequence Data; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013696:Temperature; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; C418864:ferritin-like protein, Listeria; D005293:Ferritins