title: Utility of restriction fragment analysis for typing herpes simplex virus amplicons following PCR of targets in the DNA polymerase gene.
abstract: We compared the utility of restriction endonuclease cleavage to type herpes simplex virus DNA polymerase gene amplicons from two well established PCR primer sets. Amplicons typed using Ava II had a 96% correlation to type determined by monoclonal antibody staining, while amplicons typed using Drd I had a 72% correlation to type determined by monoclonal antibody staining.
authors.affiliation: Department of Pathology, Wayne State University, Detroit, Michigan, USA. rpodzor@cmb.biosci.wayne.edu
authors.forename: R P
authors.initials: RP
authors.lastname: Podzorski
authors.forename: J
authors.initials: J
authors.lastname: Baker
authors.forename: J R
authors.initials: JR
authors.lastname: Merline
authors.forename: R
authors.initials: R
authors.lastname: Qureshi
authors.forename: J E
authors.initials: JE
authors.lastname: Holsinger
mesh_terms: D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D018259:Herpesvirus 1, Human / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D018258:Herpesvirus 2, Human / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D004279:DNA, Viral; D004259:DNA-Directed DNA Polymerase