Distribution of somatostatin receptor type 1 in the rat. An immunohistochemical study.
Somatostatin receptor type 1 (SSTR-1) was identified immunohistochemically in the rat tissues using anti-peptide antiserum. Anti-somatostatin receptor antiserum was raised in New Zealand white rabbits immunized with a conjugate of synthetic SSTR-1 peptide (41-52) with bovine serum albumin. Immunohistochemical analysis was performed by the ABC method. SSTR-1 immunoreactivity was visualized in the central nervous system, anterior pituitary, mucosa of stomach and duodenum, Auerbach's nervous branch and Mysner's nervous branch of gastrointestinal tract and pancreas. Significant staining was detected in neural perikarya, axon and dendrite. When using antiserum preincubated with synthetic SSTR-1 peptide (41-52) or rat anterior pituitary homogenated which contains SSTR-1, no significantly stained anterior pituitary cells or neurons in the hypothalamus were detected. These findings suggest that SSTR-1 is widely distributed and that this method is valuable in studying the distribution of somatostatin receptor in rats.
Mitsuma
T
The Four Department of Internal Medicine, Konanshowa Hospital, Konan, Aichi, Japan.
Rhue
N
Sobue
G
Kayama
M
Yokoi
Y
Izumi
M
Adachi
K
Hirooka
Y
Nogimori
T
Sakai
J
Sugie
I