title: Molecular cloning and biological characterization of a novel murine lymphoid growth factor.
abstract: Using a bioassay consisting of the proliferation of a murine B cell line, a cDNA of a gene whose product supports the growth of that cell line was isolated from a thymic stromal cell line. This factor, termed thymic stromal lymphopoietin (TSLP), is a protein of 140 amino acids. The gene encoding TSLP was mapped to murine chromosome 18. Purified recombinant TSLP supported the growth of pre-B cell colonies in vitro, but had no myelopoietic activity. TSLP had comitogenic activity for fetal thymocytes, but was not as potent as interleukin 7 in lobe submersion cultures. Injection of TSLP into neonatal mice induced the expansion of B220(+)BP-1(+) pre-B cells.
authors.affiliation: Department of Molecular Biology, Immunex Corporation, Seattle, Washington 98101, USA. sims@immunex.com
authors.forename: J E
authors.initials: JE
authors.lastname: Sims
authors.forename: D E
authors.initials: DE
authors.lastname: Williams
authors.forename: P J
authors.initials: PJ
authors.lastname: Morrissey
authors.forename: K
authors.initials: K
authors.lastname: Garka
authors.forename: D
authors.initials: D
authors.lastname: Foxworthe
authors.forename: V
authors.initials: V
authors.lastname: Price
authors.forename: S L
authors.initials: SL
authors.lastname: Friend
authors.forename: A
authors.initials: A
authors.lastname: Farr
authors.forename: M A
authors.initials: MA
authors.lastname: Bedell
authors.forename: N A
authors.initials: NA
authors.lastname: Jenkins
authors.forename: N G
authors.initials: NG
authors.lastname: Copeland
authors.forename: K
authors.initials: K
authors.lastname: Grabstein
authors.forename: R J
authors.initials: RJ
authors.lastname: Paxton
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects*; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D016207:Cytokines / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*; D018076:DNA, Complementary / Q000302:isolation & purification; D005260:Female; D006410:Hematopoiesis / Q000187:drug effects*; D015851:Interleukin-7 / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D016207:Cytokines; D018076:DNA, Complementary; D015851:Interleukin-7; D012333:RNA, Messenger; D011994:Recombinant Proteins