title: Immunoblot analysis of IgA antibodies to Naegleria fowleri in human saliva and serum.
abstract: The objective of this study was to evaluate the secretory IgA (SIgA) antibody response to Naegleria fowleri (Nf) in individuals living in a parasite endemic area. Saliva and serum samples were obtained from both healthy subjects and patients suffering from a respiratory illness (chronic bronchitis or rhinitis) and were analyzed by immunoblot assay. SIgA from the patients' samples recognized more intensely a greater number of Nf proteins than did SIgA from the healthy control group. The proteins more frequently recognized were those with a molecular weight of 171, 107, 102, 62, 50, 46, and 10 kDa. Some IgA antibodies recognized proteins from Nf and Entamoeba histolytica (Eh) of similar molecular weight. These results suggest that some of those antibodies could have been elicited by a previous intestinal infection with Eh. Through the common mucosal immune system the IgA B-cells activated by Eh antigens can be disseminated to all the mucosae, including the nasal mucosa. SIgA antibodies recognizing Nf proteins, induced either by specific immunization or by cross-reaction, could participate in the resistance to the infection, probably by inhibiting the adherence of Nf trophozoites to the nasal mucosa.
authors.affiliation: Department of Biochemistry, School of Medicine, National Polytechnic Institute, Plan de San Luis y Díaz Mirón, Mexico, DF.
authors.forename: V
authors.initials: V
authors.lastname: Rivera-Aguilar
authors.forename: D
authors.initials: D
authors.lastname: Hernández-Martínez
authors.forename: S
authors.initials: S
authors.lastname: Rojas-Hernández
authors.forename: G
authors.initials: G
authors.lastname: Oliver-Aguillón
authors.forename: V
authors.initials: V
authors.lastname: Tsutsumi
authors.forename: N
authors.initials: N
authors.lastname: Herrera-González
authors.forename: R
authors.initials: R
authors.lastname: Campos-Rodríguez
mesh_terms: D000562:Amebiasis / Q000097:blood* / Q000175:diagnosis; D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis*; D000953:Antigens, Protozoan / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting; D007070:Immunoglobulin A / Q000032:analysis*; D016848:Naegleria fowleri / Q000276:immunology* / Q000302:isolation & purification; D012463:Saliva / Q000276:immunology / Q000469:parasitology*
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007070:Immunoglobulin A