An in vitro assay to measure phagocytosis in striped bass hybrids (Morone saxatilis x Morone chrysops).
An in vitro phagocytosis assay was developed for hybrid striped bass (Morone saxatilis x Morone chrysops), using cells collected from the peritoneal cavity of this fish. The findings indicated that: (1) 10 days following a single intraperitoneal injection (1 ml) of Freund's incomplete adjuvant (FIA) was an appropriate time for collecting suitable working concentrations (5.3+/-4.0 x 10(7) cells ml(-1)) of peritoneal phagocytes (83.7+/-1.5% macrophages) from these hybrids held at 23 degrees C; (2) these cells phagocytosed latex beads (polystyrene microspheres 3.12 microm in diameter) after 30 min of in vitro incubation at room temperature (25+/-1 degrees C). The phagocytic ability and phagocytic capacity in a washed adherent layer exposure system were 67.2+/-2.76% and 4.14+/-0.35 beads phagocyte(-1), respectively. These results strongly suggest that a simple methodology, including baseline data serving as guidelines, is now available for conducting in vitro phagocytosis assays in this hybrid.
Polonio
R E
RE
Department of Fisheries, Animal & Veterinary Sciences, University of Rhode Island, Kingston 02881, USA. rpol8053@postoffice.uri.edu
Wolke
R E
RE
MacLean
S A
SA
Sperry
J F
JF
D000818:Animals; D001492:Bass / Q000276:immunology*; D002448:Cell Adhesion; D002452:Cell Count; D002470:Cell Survival; D017737:Macrophages, Peritoneal / Q000276:immunology; D008863:Microspheres; D010529:Peritoneal Cavity; D010587:Phagocytosis; D013048:Specimen Handling / Q000379:methods / Q000662:veterinary*