Liquid chromatographic analysis of all-trans-retinyl palmitate, beta-carotene, and vitamin E in fortified foods and the extraction of encapsulated and nonencapsulated retinyl palmitate.
A liquid chromatographic method is described for the analysis of natural vitamin E homologues, all-rac-alpha-tocopheryl acetate, retinyl palmitate (encapsulated and nonencapsulated), and beta-carotene in various fortified foods. The vitamins are extracted in 2-propanol and hexane without saponification and quantitated by normal phase chromatography with fluorescence and visible detection. The sample components were identified using an on-line three-dimensional photodiode array detector, which permitted profiling of the 190-800 nm absorption spectrum of any chromatographic peak. The method showed linearity for the analytes in their respective calibration ranges. The percent recoveries for retinyl palmitate using starch- and gelatin-encapsulated standards were 101.0 +/- 1.0 and 100.1 +/- 0.9, respectively. The method measures six or more analytes in a single injection and differentiates between natural and synthetic forms of vitamin E.
Ye
L
L
Department of Food Science and Technology, University of Georgia, Athens, Georgia 30602, USA.
Landen
W O
WO
Eitenmiller
R R
RR
D002853:Chromatography, Liquid / Q000379:methods*; D004224:Diterpenes; D005527:Food, Fortified / Q000032:analysis*; D000084562:Retinyl Esters; D014801:Vitamin A / Q000031:analogs & derivatives* / Q000032:analysis; D014810:Vitamin E / Q000032:analysis*; D019207:beta Carotene / Q000032:analysis*
D004224:Diterpenes; D000084562:Retinyl Esters; D019207:beta Carotene; D014801:Vitamin A; D014810:Vitamin E; C014794:retinol palmitate