Robertsonian metacentrics of the house musk shrew (Suncus murinus, Insectivora, Soricidae) lose the telomeric sequences in the centromeric area.
The house musk shrew, Suncus murinus, is polymorphic for five Robertsonian translocations (Rb8.17, 9.13, 10.12, 11.16, 14.15). Fluorescence in situ hybridisation with a biotin-labelled oligonucleotide, (TTAGGG)7, was performed to localise the telomeric DNA sequences at Rb chromosomes of heterozygous shrews. Hybridisation signals were observed at both ends of all chromosomes, but not at the pericentromeric areas of any of the Robertsonian metacentrics. Our results indicate a complete loss of the telomeric sequences at the fusion points of the Rb metacentrics in S. murinus.
Rogatcheva
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MB
Institute of Cytology and Genetics, Novosibirsk, Russia. rogatchrita@hotmail.com
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Borodin
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D000818:Animals; D019677:Artificial Gene Fusion; D002503:Centromere; D005260:Female; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008297:Male; D012788:Shrews / Q000235:genetics*; D016615:Telomere; D014178:Translocation, Genetic