Genotyping of Pneumocystis carinii f. sp. hominis isolates in Japan based on nucleotide sequence variations in internal transcribed spacer regions of rRNA genes.
Genotyping of Pneumocystis carinii (Pc) isolated from 24 bronchoalveolar lavage (BAL) fluid specimens in Japan was examined based on nucleotide sequence variations in internal transcribed spacer regions 1 and 2 (ITS1 and ITS2, respectively) of rRNA genes. We found 11 ITS1 genotypes including 2 novel ones and 11 ITS2 genotypes including 3 new ones. Combining the ITS1 and ITS2 genotypes resulted in 30 ITS genotypes, of which 10 are newly described in this report. Two or more genotypes in ITS regions in a specimen were observed in 16 of 24 patients. Our results will be of help for the epidemiological investigation of Pc infection.
Hosoya
N
N
Department of Infectious Diseases, Institute of Medical Science, The University of Tokyo, Japan.
Takahashi
T
T
Wada
M
M
Endo
T
T
Nakamura
T
T
Sakashita
H
H
Kimura
K
K
Ohnishi
K
K
Nakamura
Y
Y
Mizuochi
T
T
Iwamoto
A
A
D000328:Adult; D001483:Base Sequence; D005260:Female; D005800:Genes, Fungal; D016681:Genome, Fungal; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D011010:Pneumocystis / Q000235:genetics* / Q000302:isolation & purification; D012335:RNA, Ribosomal / Q000235:genetics*; D014158:Transcription, Genetic
D012335:RNA, Ribosomal