The Vancomycin biosynthetic pathway is induced in oxygen-limited Amycolatopsis orientalis (ATCC 19795) cultures that do not produce antibiotic.
Vancomycin biosynthetic capacity was stimulated in oxygen-limited bioreactor culture (6.4% DOT) of Amycolatopsis orientalis, coinciding with a down-regulation of the culture growth and protein synthesis rates. However no Vancomycin was detected. ATP production was similar in oxygen-limited and oxygen-sufficient cultures (400-150 µmole(ATP). g(biomass)(-1)). Our findings suggest a critical requirement for oxygen flux through the Vancomycin biosynthetic pathway, which is only met under nutrient-limited conditions in which oxygen is in excess.
Dunstan
GH
Microbial Products Laboratory, Biological Sciences, University of Surrey, Guildford, GU2 5x H, Surrey, UK
Avignone-Rossa
C
Langley
D
Bushell
ME