Effect of D-002 on gastric mucus composition in ethanol-induced ulcer.
This study was designed to determine the effect of D-002, a natural product isolated and purified from beeswax (Apis mellifera), on gastric mucus composition on ethanol-induced ulcer in rats. The morphology of the lesions was analysed histologically, and morphometric analysis of gastric-gland content in total glycoprotein and sulphated macromolecules were done. Oral pretreatment with D-002 at 5 and 25 mgkg(-1)1 before oral administration of ethanol at 60%, produced a significant increase in the amount of gastric mucus and total protein. The histomorphometric evaluation of the gastric damage at the same doses showed a significant increase in neutral glycoproteins and sulfated macromolecules. It is concluded that enhancement of the quantity and quality of the mucus could partly explain the gastroprotective effect of D-002.
Carbajal
D
D
Department of Pharmacology, Center of Natural Products, National Center of Scientific Research, Ciudad Habana, Cuba.
Molina
V
V
Noa
M
M
Valdés
S
S
Arruzazabala
M L
ML
Aguilar
C
C
Más
R
R
D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology; D000431:Ethanol; D005233:Fatty Alcohols / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D009093:Mucus / Q000737:chemistry / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D014885:Waxes / Q000737:chemistry
D000897:Anti-Ulcer Agents; C104511:D 002; D005233:Fatty Alcohols; D014885:Waxes; C038228:beeswax; D000431:Ethanol