Changes in protein synthesis and acid tolerance in Clostridium perfringens type A in response to acid shock.
The induction of acid-shock proteins and the degree of acid resistance conferred on Clostridium perfringens by acid shock, and the kinetics of this resistance were determined. A sublethal acid shock at pH 4.5 for 20 min increased the acid tolerance of cells at least fifteenfold. The acquired tolerance was maintained for 3 h after acid treatment. The response of the microorganism to acid shock was also examined by analysis of pulse-labeled proteins. Five acid-shock proteins (molecular weights 120, 84, 58, 45 and 17 kDa) were identified by polyacrylamide gel electrophoresis.
Villarreal
L
L
Faculty of Biological Sciences, Autonomous University of Nuevo León, San Nicolás, México.
Heredia
N L
NL
García
S
S
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D003016:Clostridium perfringens / Q000145:classification / Q000187:drug effects / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration
D001426:Bacterial Proteins