[A portable photometer-reflectometer for quantitative recording of results of immunoenzyme analysis].
A portable optoelectronic reflectometric photocolorimeter was designed. The photocolorimeter implements two operating modes (transmission and reflection), and its performance was tested in two systems of testosterone immunoenzyme assay: microplate ELISA and membrane dot-ELISA. The detection threshold for microplate ELISA and membrane dot-ELISA was 0.1 and 0.6 ng/ml, respectively. The coefficient of correlation between the photocolorimeter readings and conventional photometric methods is r = 0.999. Relative standard deviation of the results of photocolorimetric measurements (n = 4) within the optical density range from 0.03 to 1.00 ranged from 3.4 to 0.7%. Simple and versatile design of the photocolorimeter make it appropriate for testing various substances both in laboratory settings and in the field.
Shishkin
Iu L
IuL
Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Zherdev
A V
AV
Dzantiev
B B
BB
Zolotov
Iu A
IuA
D003124:Colorimetry / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D010783:Photometry / Q000379:methods*; D015203:Reproducibility of Results; D013057:Spectrum Analysis / Q000379:methods*; D013739:Testosterone / Q000032:analysis
D013739:Testosterone