Protein-protein interactions mapped by artificial proteases: where sigma factors bind to RNA polymerase.
Interactions between proteins are important to understand but difficult to study. Conjugating a protein to a small artificial protease endows it with the ability to cut other proteins where it binds to them. Analysing the sites cut on the target proteins leads to new understanding of the structure of each complex. The binding of sigma factors to a common region on RNA polymerase provides an example.
Datwyler
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Dept of Chemistry, University of California, One Shields Avenue, Davis, CA 95616-5295, USA.
Meares
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CF
D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D015153:Blotting, Western; D003545:Cysteine / Q000737:chemistry / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000378:metabolism*; D004492:Edetic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000737:chemistry / Q000378:metabolism*; D007502:Iron Chelating Agents / Q000737:chemistry / Q000378:metabolism; D008956:Models, Chemical; D008958:Models, Molecular; D009942:Organometallic Compounds / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D017433:Protein Structure, Secondary; D012808:Sigma Factor / Q000737:chemistry / Q000378:metabolism*
D001426:Bacterial Proteins; D007502:Iron Chelating Agents; D009942:Organometallic Compounds; D012808:Sigma Factor; C042007:1-(4-bromoacetamidobenzyl)EDTA; D004492:Edetic Acid; D012321:DNA-Directed RNA Polymerases; D010450:Endopeptidases; D003545:Cysteine