title: Evolution of a D2 dopamine receptor intron within the great apes and humans.
abstract: Although direct DNA sequencing may allow rapid and high quality comparative phylogenetic analyses among species, such an approach may not be the most efficient method by which to make a large number of cross-species comparisons. We illustrate the use of Denaturing Gradient Gel Electrophoresis (DGGE) to screen a D2 Dopamine Receptor intron for DNA sequence variation, both within and between closely related species, in order to infer their evolutionary relationships. Our results suggest that: a) humans have less genetic variation than the great apes; b) pygmy chimpanzees have less genetic variation than common chimpanzees; and c) DNA sequence comparative analyses of primates require adequate sampling, both in number and in geographical range.
authors.affiliation: Department of Anthropology, Yale University, New Haven, CT 06520, USA.
authors.forename: A S
authors.initials: AS
authors.lastname: Deinard
authors.forename: K K
authors.initials: KK
authors.lastname: Kidd
mesh_terms: D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary; D019143:Evolution, Molecular; D014644:Genetic Variation; D006071:Gorilla gorilla / Q000235:genetics; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D019974:Pan paniscus / Q000235:genetics; D002679:Pan troglodytes / Q000235:genetics; D011148:Pongo pygmaeus / Q000235:genetics; D017448:Receptors, Dopamine D2 / Q000145:classification / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D017448:Receptors, Dopamine D2