Uptake of bismuth in motor neurons of mice after single oral doses of bismuth compounds.
Bismuth, a component of many gastrointestinal medications, is a heavy metal little studied as regards nervous system uptake. We were interested to see if low doses of intragastric bismuth entered the nervous system, and if dietary selenium influenced the amount of bismuth detected. Mice were given 40 to 1200 mg/kg of bismuth subnitrate (BSN), bismuth subsalicylate (BSS), colloidal bismuth subcitrate (CBS), or ranitidine bismuth citrate (RBC) intragastrically. Mice on low- or high-selenium diets were given 4 to 32 mg/kg of bismuth from RBC. One week later, sections of nervous tissue were stained with autometallography to detect bismuth grains (Bi(AMG)). Bismuth was found in neurons with axons outside of the nervous system, in particular motor neurons, and in cells outside the blood-brain barrier. The lowest bismuth dose which resulted in Bi(AMG) in motor neurons was 696 mg/kg from BSN, 57 mg/kg from BSS, 29 mg/kg from CBS, and 26 mg/kg from RBC. No bismuth was seen in motor neurons of mice on the low-selenium diet. Intragastric doses of bismuth therefore enter mouse motor neurons, and the amount detectable varies with dietary selenium.
Pamphlett
R
R
Molecular Neurotoxicology Laboratory, Department of Pathology, University of Sydney, Sydney, Australia. rogerp@med.usyd.edu.au
Stoltenberg
M
M
Rungby
J
J
Danscher
G
G
D000284:Administration, Oral; D000818:Animals; D001729:Bismuth / Q000493:pharmacokinetics*; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D004032:Diet; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009046:Motor Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009942:Organometallic Compounds / Q000493:pharmacokinetics*; D010865:Pilot Projects; D012459:Salicylates / Q000493:pharmacokinetics; D012643:Selenium / Q000008:administration & dosage
D009942:Organometallic Compounds; D012459:Salicylates; C015715:bismuth subsalicylate; C010804:bismuth subnitrate; D012643:Selenium; C002791:bismuth tripotassium dicitrate; D001729:Bismuth