The recovery of plasma cholinesterase and erythrocyte acetylcholinesterase activity in workers after over-exposure to dichlorvos.
Plasma cholinesterase and erythrocyte acetylcholinesterase activities have a long history of use in monitoring both workers at risk of organophosphorus pesticide (OP) exposure and in investigating accidental exposures to OPs. On account of wide inter-individual variation, the establishment of unexposed, baseline enzyme activities is necessary for accurate interpretation. This paper describes the rate of recovery of the two enzymes' activity after substantial over-exposure of eight subjects to the OP dichlorvos. Plasma cholinesterase activity, immediately after exposure, was substantially more inhibited than erythrocyte acetylcholinesterase activity. The plasma enzyme activity showed an exponential pattern of recovery with a half-life of around 12 days, so recovery was essentially complete after about 50 days. This reported half-life of recovery is consistent with the reported de novo synthesis rate of plasma cholinesterase. The mean recovery of erythrocyte acetylcholinesterase activity appeared linear over time, attaining unexposed activity after about 82 days, which is somewhat shorter than the life-span of erythrocytes. These indicate the sort of time period, after an OP incident, before a valid unexposed level can be established in an individual; and substantiate the guidance given in the Health & Safety Executive's document MS17 on a minimum period of 60 days without exposure in order to establish pre-exposure baseline levels.
Mason
H J
HJ
Health & Safety Laboratory, Sheffield, UK. howard.mason@hsl.gov.uk
D000110:Acetylcholinesterase / Q000097:blood; D015415:Biomarkers / Q000097:blood; D002802:Cholinesterases / Q000097:blood*; D004006:Dichlorvos / Q000506:poisoning*; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D019570:Inhalation Exposure; D007306:Insecticides / Q000506:poisoning*; D016273:Occupational Exposure / Q000009:adverse effects*
D015415:Biomarkers; D007306:Insecticides; D004006:Dichlorvos; D000110:Acetylcholinesterase; D002802:Cholinesterases