Characterization and mutagenesis of fur gene from Burkholderia pseudomallei.
A homolog of the ferric uptake regulator gene (fur) was isolated from Burkholderia pseudomallei (Bp) by a reverse genetic technique. Sequencing of a 2.2kb DNA fragment revealed an open reading frame with extensive homology to bacterial Fur proteins. The cloned gene encodes a 16kDa protein that cross-reacts with a polyclonal anti-Escherichia coli Fur serum. The transcription start site was determined by the primer extension technique. Expression analysis of fur showed no increased fur mRNA levels in response to various stresses and iron conditions. A positive selection procedure involving the isolation of manganese-resistant mutants was used to isolate mutants that produce altered Fur proteins. Sequencing of a fur mutant revealed a nucleotide change (G to A) converting a conserved amino acid arginine-69 to histidine. The fur missense mutant produced an elevated level of siderophore that could be complemented by a multicopy plasmid carrying the Bp fur. Interestingly, Fur was found to play roles as a positive regulator of FeSOD and peroxidase. The mutant showed a decreased activity of FeSOD and peroxidase, which could be important in its pathogenicity and survival in macrophages.
Loprasert
S
S
Laboratory of Biotechnology, Chulabhorn Research Institute, Lak Si, 10210, Bangkok, Thailand. suvit@tubtim.cri.or.th
Sallabhan
R
R
Whangsuk
W
W
Mongkolsuk
S
S
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D016957:Burkholderia pseudomallei / Q000187:drug effects / Q000235:genetics*; D002712:Chlorides / Q000494:pharmacology; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D007501:Iron / Q000494:pharmacology; D017895:Manganese Compounds / Q000494:pharmacology; D008969:Molecular Sequence Data; D016296:Mutagenesis / Q000187:drug effects; D009154:Mutation; D016877:Oxidants / Q000494:pharmacology; D010544:Peroxidases / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012329:RNA, Bacterial / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013482:Superoxide Dismutase / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects
D001426:Bacterial Proteins; D002712:Chlorides; D004269:DNA, Bacterial; D017895:Manganese Compounds; D016877:Oxidants; D012329:RNA, Bacterial; D012097:Repressor Proteins; C068477:ferric uptake regulating proteins, bacterial; D007501:Iron; D010544:Peroxidases; D013482:Superoxide Dismutase; C025340:manganese chloride