title: Molecular recognition specificity of Bacillus globigii spore antibodies.
abstract: Western blotting methods have been used to assess the specificity of polyclonal antibodies raised against Bacillus globigii spore and vegetative cell preparations. None of the antibodies studied were completely species-specific in their recognition of spore surface epitopes. One polyclonal serum recognized several spore surface epitopes and demonstrated limited cross-reaction with the spore surface of the near-neighbour species B. subtilis. A second polyclonal serum, raised against aged spore antigens, recognized damaged spore epitopes primarily. Both of these antibodies also cross-reacted with vegetative cell epitopes present in all four Bacillus species (B. globigii, B. subtilis, B. cereus and B. anthracis) studied.
authors.affiliation: Division of Biochemistry and Molecular Biology, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3202, USA.
authors.forename: P
authors.initials: P
authors.lastname: Longchamp
authors.forename: T
authors.initials: T
authors.lastname: Leighton
mesh_terms: D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology; D001407:Bacillus / Q000254:growth & development / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology; D015153:Blotting, Western; D013171:Spores, Bacterial / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins