Characterization of Solt, a novel SoxLZ/Sox6 binding protein expressed in adult mouse testis.
SoxLZ/Sox6, a member of the Sox protein family, contains a leucine zipper motif in addition to an HMG box, which is its DNA binding domain. Here we have identified a novel SoxLZ/Sox6 binding protein, termed Solt, which we obtained independently using both a far-Western blot and a yeast two-hybrid screen. Like SoxLZ/Sox6 mRNA, Solt mRNA was exclusively expressed in the testis in mouse. Solt contains an unusual leucine zipper, which bound to the leucine zipper region of SoxLZ/Sox6 in vitro. In transient transfection assays in CHO cells with SoxLZ/Sox6 containing the transactivational region of herpes simplex virus VP16, expression of a reporter gene that carries a cis binding region for Sox proteins was significantly enhanced by the co-expression of Solt and Ca(2+)/calmodulin-dependent protein kinase IV.
Yamashita
A
A
Department of Biosciences, School of Science, Kitasato University, 1-15-1 Kitasato, Sagamihara, 228, Kanagawa, Japan.
Ito
M
M
Takamatsu
N
N
Shiba
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D016466:CHO Cells; D054734:Calcium-Calmodulin-Dependent Protein Kinase Type 4; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics / Q000378:metabolism; D006224:Cricetinae; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004789:Enzyme Activation; D029941:Fish Proteins; D005786:Gene Expression Regulation; D017930:Genes, Reporter / Q000235:genetics; D017405:Herpes Simplex Virus Protein Vmw65 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006609:High Mobility Group Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016350:Leucine Zippers; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D009928:Organ Specificity; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D055747:SOX Transcription Factors; D055755:SOXD Transcription Factors; D013737:Testis / Q000166:cytology / Q000378:metabolism*; D014157:Transcription Factors; D014162:Transfection; D020798:Two-Hybrid System Techniques
D004268:DNA-Binding Proteins; D029941:Fish Proteins; D017405:Herpes Simplex Virus Protein Vmw65; D006609:High Mobility Group Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D055747:SOX Transcription Factors; D055755:SOXD Transcription Factors; C414366:Solt protein, mouse; C095939:Sox6 protein, mouse; D014157:Transcription Factors; D054734:Calcium-Calmodulin-Dependent Protein Kinase Type 4; D017871:Calcium-Calmodulin-Dependent Protein Kinases; C517685:Camk4 protein, mouse