title: Turkey and chicken interferon-gamma, which share high sequence identity, are biologically cross-reactive.
abstract: Turkey and chicken interferon-gamma (IFN-gamma) share high identity (96.3% and 97% at the nucleotide and amino acid level, respectively). As such, we predicted that they would be functionally cross-reactive. To test this hypothesis, we produced recombinant turkey and chicken IFN-gamma, and compared their biological properties. Recombinant turkey and chicken IFN-gamma both induce HD11 cells (a chicken macrophage cell line) and LSTC-IAH30 cells (ALV-J-transformed turkey macrophages) to produce nitric oxide (NO), as measured in an avian IFN-gamma bioassay. Polyclonal and monoclonal antibodies, capable of neutralising the effect of chicken IFN-gamma on HD11 cells, were also shown to inhibit the activity of turkey IFN-gamma on these cells. The antibody neutralisation effect on both turkey and chicken IFN-gamma was shown by a significant reduction in NO production by HD11 cells when the neutralising antibodies were present in the bioassay. FACS analysis showed that HD11 and LSTC-IAH30 cells share some cell surface markers.
authors.affiliation: Institute for Animal Health, Compton, Berkshire, UK. michelle.lawson@bbsrc.ac.uk
authors.forename: S
authors.initials: S
authors.lastname: Lawson
authors.forename: L
authors.initials: L
authors.lastname: Rothwell
authors.forename: B
authors.initials: B
authors.lastname: Lambrecht
authors.forename: K
authors.initials: K
authors.lastname: Howes
authors.forename: K
authors.initials: K
authors.lastname: Venugopal
authors.forename: P
authors.initials: P
authors.lastname: Kaiser
mesh_terms: D000818:Animals; D019556:COS Cells; D002478:Cells, Cultured; D002645:Chickens / Q000378:metabolism*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005434:Flow Cytometry / Q000662:veterinary; D007371:Interferon-gamma / Q000737:chemistry* / Q000276:immunology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D017386:Sequence Homology, Amino Acid; D014422:Turkeys / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D007371:Interferon-gamma