Viral double-stranded RNAs of Eimeria spp. of the domestic fowl: analysis of genetic relatedness and divergence among various strains.
Genetic relatedness was examined among putative viral double-stranded RNA (dsRNA) genomes of Eimeria acervilina, E. maxima, and E. necatrix using Northern hybridization. No cross-hybridization was found among these dsRNAs, revealing little sequence homology, if any. In addition, dsRNAs from eight E. acervulina strains collected from different localities in the United States were also examined for genetic relatedness. The putative viral dsRNAs from these eight strains, including the Guelph strain, hybridized with one another to varying degrees, indicating that they are related but divergent.
Lee
S
S
Department of Pathobiology, University of Guelph, Ontario, Canada.
Fernando
M A
MA
D000818:Animals; D015152:Blotting, Northern; D002645:Chickens / Q000469:parasitology*; D003048:Coccidiosis / Q000469:parasitology*; D004539:Eimeria / Q000821:virology*; D016679:Genome, Viral; D009693:Nucleic Acid Hybridization; D011201:Poultry Diseases / Q000469:parasitology*; D012328:RNA Viruses / Q000235:genetics* / Q000302:isolation & purification; D012330:RNA, Double-Stranded / Q000032:analysis* / Q000145:classification; D012367:RNA, Viral / Q000032:analysis* / Q000145:classification
D012330:RNA, Double-Stranded; D012367:RNA, Viral