[Effect of ethanol on the activity of the energy-dependent Ca2+-transporting system of myometrial cells].
In order of estimating some regularities of ethanol direct (effectory) effect to transmembrane calcium metabolism in the myometrium the action of this substance on the energy-dependent Ca(2+)-transporting systems of the uterine myocytes subcellular structures has been studied. The systems of Mg2+, ATP-dependent Ca2+ transport regarding their sensitivity to ethanol inhibitory effect were displayed as satisfying the following sequences: endoplasmic reticulum calcium pump > plasma membrane solubilized Ca2+, Mg2+, ATP-ase > mitochondrial Ca(2+)-accumulating system = plasma membrane calcium pump. Alongside with the latter, the oxytocin-insensitive component of Mg2+, ATP-dependent Ca2+ accumulation in the endoplasmic reticulum was defined to be less resistant to inhibitory effect of ethanol if compared with the oxytocin-sensitive one. On the base of the data received some mechanisms of ethanol effectory action on the intracellular calcium homeostasis in the myometrium cells are under the discussion.
Babich
L G
LG
A. V. Palladin Institute of Biochemistry, National Academy of Science, Kyiv.
Shlykov
S G
SG
Borisova
L A
LA
Slinchenko
N N
NN
Bratkova
N F
NF
Kosterin
S A
SA
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D017136:Ion Transport; D009215:Myometrium / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D013347:Subcellular Fractions / Q000378:metabolism; D013552:Swine
D000431:Ethanol; D000252:Calcium-Transporting ATPases; D002118:Calcium