[Insect-resistant transgenic poplar expressing AaIT gene].
Insect-specific scorpion neurotoxin AaIT gene inserted into a binary vector was transferred into a hybrid poplar clone N-106(P. deltoides x P. simonii) growing in the Southern of China. We obtained sixty-two regenerated plants by Agrobacterium tumefaciens transferring system. PCR and PCR-Southern analysis showed that AaIT gene was incorporated into the genome of some recovered poplar plants. One of the transformed plants named A5 was significantly resistant to feeding by first instar larvae of Lymantria dispar, compared with the untransformed control plant. It caused a decrease in leaf consumption by larvae, a lower larval weight gain and a higher larval motality rate of Lymantria dispar. ELISA analysis proved that AaIT gene was expressed in this transfomed poplar plant.
Wu
N F
NF
Biotechnology Research Center, Chinese Academy of Agricultural Sciences, Beijing.
Sun
Q
Q
Yao
B
B
Fan
Y L
YL
Rao
H Y
HY
Huang
M R
MR
Wang
M X
MX
D000818:Animals; D015139:Blotting, Southern; D007313:Insecta; D007306:Insecticides; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D012604:Scorpion Venoms / Q000235:genetics*; D014170:Transformation, Genetic
D007306:Insecticides; D012604:Scorpion Venoms; C065634:AaIT neurotoxin, Androctonus australis