Cloning and sequencing of the levansucrase gene from Acetobacter xylinum NCI 1005.
The levansucrase gene (lsxA) was cloned from the genomic DNA of Acetobacter xylinum NCI 1005, and the nucleotide sequence of the lsxA gene (1,293 bp) was determined. The deduced amino acid sequence of the lsxA gene showed 57.4% and 46.2% identity with the levansucrases from Zymomonas mobilis and Erwinia amylovora, respectively, while only 35.2% identity with that from Acetobacter diazotrophicus. The gene product of lsxA (LsxA) that was overproduced in E. coli coded for a polypeptide of molecular mass 47 kDa. The LsxA released glucose and produced polysaccharide from sucrose, the structure of which was analyzed by nuclear magnetic resonance spectroscopy and determined to be a beta-(2,6)-linked polyfructan.
Tajima
K
K
Division of Molecular Chemistry, Graduate School of Engineering, Hokkaido University, Sapporo, Japan. tajima@dove-mc.eng.hokudai.ac.jp
Tanio
T
T
Kobayashi
Y
Y
Kohno
H
H
Fujiwara
M
M
Shiba
T
T
Erata
T
T
Munekata
M
M
Takai
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004885:Erwinia / Q000235:genetics; D004926:Escherichia coli / Q000378:metabolism; D041822:Gluconacetobacter xylinus / Q000235:genetics*; D005947:Glucose / Q000378:metabolism; D006602:Hexosyltransferases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010455:Peptides / Q000737:chemistry; D011134:Polysaccharides / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013395:Sucrose / Q000378:metabolism; D013997:Time Factors; D017355:Zymomonas / Q000235:genetics
D010455:Peptides; D011134:Polysaccharides; D013395:Sucrose; D006602:Hexosyltransferases; C021513:levansucrase; D005947:Glucose