Impact of missense mutations on biosynthesis of myeloperoxidase.
We have examined the biosynthesis of normal and mutant forms of myeloperoxidase (MPO) in order to gain insights into the critical features of normal biogenesis of MPO. The expression of wild-type and mutant forms of MPO in a stably transfected cell line devoid of endogenous MPO as well as in established human promyelocytic cell lines has allowed understanding of several features of MPO biosynthesis. It is clear that heme insertion into apoproMPO is necessary for proper folding, egress from the endoplasmic reticulum (ER), and eventual entry into the maturation pathway. In addition, molecular chaperones calreticulin and calnexin interact with normal MPO precursors in a sequential and regulated fashion. Studies of naturally occurring mutants, specifically missense mutations underlying inherited MPO deficiency, and mutations in putatively important residues in MPO have highlighted special features of the ER quality control system in the context of MPO biosynthesis. With identification of additional genotypes of MPO deficiency and the recent solution of MPO crystal structure at 1.8 A, this approach provides a powerful technique to assess structure-function relationships in MPO that are likely applicable to other members of the family of animal peroxidases.
Nauseef
W M
WM
Inflammation Program and Department of Medicine, University of Iowa, and Veterans Affairs Medical Center at Iowa City, 52422, USA. william-nauseef@uiowa.edu
McCormick
S
S
Goedken
M
M
D000818:Animals; D002135:Calcium-Binding Proteins / Q000502:physiology; D037281:Calnexin; D037282:Calreticulin; D004721:Endoplasmic Reticulum / Q000502:physiology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005838:Genotype; D006801:Humans; D007930:Leucine / Q000031:analogs & derivatives / Q000494:pharmacology; D018832:Molecular Chaperones / Q000502:physiology; D020125:Mutation, Missense; D009195:Peroxidase / Q000096:biosynthesis* / Q000172:deficiency / Q000235:genetics; D012261:Ribonucleoproteins / Q000502:physiology
D002135:Calcium-Binding Proteins; D037282:Calreticulin; D004791:Enzyme Inhibitors; D018832:Molecular Chaperones; D012261:Ribonucleoproteins; C116500:acetyl-leucinal-nor-leucinal; D037281:Calnexin; D009195:Peroxidase; D007930:Leucine