title: Differential expression of CD34 in normal colorectal tissue, peritumoral inflammatory tissue, and tumour stroma.
abstract: OBJECTIVE
  To investigate the role of CD34 positive stromal cells, namely dendritic interstitial cells, in the desmoplastic stroma formation of malignant epithelial neoplasms the distribution of CD34 positive stromal cells was examined in human colorectal adenocarcinomas, peritumoral inflammatory tissue, and normal tissue.
  
  
  METHODS
  Forty one surgically resected human colorectal adenocarcinomas and their corresponding peritumoral inflammatory and normal tissues were examined. To distinguish CD34 positive stromal cells from vascular endothelial cells, immunostaining for both CD34 and CD31 was performed. The distribution of myofibroblasts was also analysed immunohistochemically, and double staining with CD34 and alpha smooth muscle actin (ASMA) was performed.
  
  
  RESULTS
  Most of the stromal cells in the normal colorectal submucosa, muscularis propria, subserosa, and perirectal tissue were positive for CD34. In contrast, the peritumoral inflammatory tissue and the tumour stroma had no CD34 positive stromal cells. The distribution of myofibroblasts was almost the same as in the aforementioned series. No stromal cells double positive for CD34 and ASMA were detected in the peritumoral inflammatory tissues.
  
  
  CONCLUSIONS
  Most stromal fibroblasts are CD34 positive stromal cells (dendritic interstitial cells). In colorectal adenocarcinomas, a lack of CD34 expression in stromal cells is associated with desmoplastic reaction.
authors.affiliation: Department of Pathology, Kure National Hospital, Hiroshima, Japan. nakayamh@kure-nh.go.jp
authors.forename: H
authors.initials: H
authors.lastname: Nakayama
authors.forename: H
authors.initials: H
authors.lastname: Enzan
authors.forename: E
authors.initials: E
authors.lastname: Miyazaki
authors.forename: N
authors.initials: N
authors.lastname: Kuroda
authors.forename: K
authors.initials: K
authors.lastname: Naruse
authors.forename: M
authors.initials: M
authors.lastname: Hiroi
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D018952:Antigens, CD34 / Q000378:metabolism*; D000951:Antigens, Neoplasm / Q000378:metabolism*; D003106:Colon / Q000166:cytology / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology; D005347:Fibroblasts / Q000473:pathology; D006801:Humans; D012007:Rectum / Q000166:cytology / Q000378:metabolism; D017154:Stromal Cells / Q000378:metabolism
chemical_list: D018952:Antigens, CD34; D000951:Antigens, Neoplasm