Immunological characterization of 130 kDa phospholipase C-beta 4 isozyme in rat cerebellar Purkinje cells.
We have developed a monoclonal antibody, beta4-22-9 that recognizes an epitope on pleckstrin homology (PH) domain (1-136 amino acids) located at the amino terminus of rat 130 kDa phospholipase C-beta4 (PLC-beta4). Tissue distribution of 130 kDa PLC-beta4 was determined with this monoclonal antibody. One hundred and thirty kiloDalton PLC-beta4 was exclusively expressed in the nervous tissues including cerebellum and cerebrum, especially at very high levels in cerebellum. The expression of 130 kDa PLC-beta4 in cerebellum was gradually increased from 5 days after birth to adulthood. In the adult cerebellum, especially high level of 130 kDa PLC-beta4 was localized on the periphery of Purkinje cells in the patch-like pattern. These data implies that 130 kDa PLC-beta4 may a role in cerebellar Purkinje cells.
Min
D S
DS
Department of Physiology, College of Medicine, Catholic University, Seoul, South Korea.
Kim
M J
MJ
Jeong
H K
HK
Lee
Y H
YH
Kim
H
H
Shin
H S
HS
Ryu
S H
SH
Suh
P G
PG
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D001792:Blood Platelets / Q000737:chemistry; D001798:Blood Proteins / Q000737:chemistry / Q000276:immunology; D002531:Cerebellum / Q000737:chemistry / Q000166:cytology / Q000201:enzymology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007527:Isoenzymes / Q000737:chemistry* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D054799:Phospholipase C beta; D010750:Phosphoproteins / Q000737:chemistry / Q000276:immunology; D017434:Protein Structure, Tertiary; D011689:Purkinje Cells / Q000201:enzymology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017386:Sequence Homology, Amino Acid; D010738:Type C Phospholipases / Q000737:chemistry* / Q000276:immunology
D000911:Antibodies, Monoclonal; D001798:Blood Proteins; D007527:Isoenzymes; D010750:Phosphoproteins; C039077:platelet protein P47; D010738:Type C Phospholipases; D054799:Phospholipase C beta; C518043:Plcb4 protein, mouse; C518044:Plcb4 protein, rat