A new 2D-TLC bioautography method for the discovery of novel antifungal agents To control plant pathogens.
A new bioassay has been developed combining the simplicity of direct bioautography with the improved chromatographic resolution of 2D-TLC. Mixtures of structurally diverse antifungal agents were tested to establish the validity and utility of this method in the discovery of new natural products with activity against agriculturally important fungal pathogens.
Wedge
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USDA-ARS, Natural Products Utilization Research Unit, National Center for Natural Products Research, University of Mississippi, University, Mississippi 38677, USA. dwedge@olemiss.edu
Nagle
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D000179:Acrylates / Q000494:pharmacology; D000814:Aniline Compounds / Q000494:pharmacology; D000935:Antifungal Agents / Q000302:isolation & purification* / Q000494:pharmacology; D001203:Ascomycota / Q000187:drug effects*; D001562:Benzimidazoles / Q000494:pharmacology; D001681:Biological Assay; D002215:Captan / Q000494:pharmacology; D002219:Carbamates; D002855:Chromatography, Thin Layer; D020231:Colletotrichum / Q000187:drug effects*; D004125:Dimethyldithiocarbamate / Q000494:pharmacology; D005659:Fungicides, Industrial / Q000494:pharmacology; D006146:Guanidines / Q000494:pharmacology; D008344:Maneb / Q000494:pharmacology; D008689:Methacrylates; D009570:Nitriles / Q000494:pharmacology; D009578:Nitrobenzenes; D010080:Oxazoles / Q000494:pharmacology; D010879:Piperazines / Q000494:pharmacology; D010935:Plant Diseases / Q000382:microbiology*; D010944:Plants / Q000382:microbiology*; D011743:Pyrimidines / Q000494:pharmacology; D000073739:Strobilurins; D013827:Thiabendazole / Q000494:pharmacology; D013875:Thiophanate / Q000494:pharmacology; D014230:Triazoles / Q000494:pharmacology
D000179:Acrylates; D000814:Aniline Compounds; D000935:Antifungal Agents; D001562:Benzimidazoles; D002219:Carbamates; D004125:Dimethyldithiocarbamate; D005659:Fungicides, Industrial; D006146:Guanidines; D008689:Methacrylates; D009570:Nitriles; D009578:Nitrobenzenes; D010080:Oxazoles; D010879:Piperazines; D011743:Pyrimidines; D000073739:Strobilurins; D014230:Triazoles; D008344:Maneb; C032910:triadimefon; C006921:dodine; C087114:tebuconazole; D013875:Thiophanate; D002215:Captan; C004354:dicloran; C006698:carbendazim; C005806:tetrachloroisophthalonitrile; C025643:vinclozolin; C012591:triforine; D013827:Thiabendazole; C087670:azoxystrobin; C005805:quintozene; C011251:ferbam