Expression of rat uncoupling protein family mRNA levels by chronic treatment with thyroid hormone.
The present study was conducted to assess the effects of chronic treatment with triiodothyronine (T3) on mRNA expression of uncoupling protein (UCP) family in male Wistar King A rats. Subcutaneous injection of T3 (37 nmol/body weight 100 g) over 7 d increased mRNA expression of UCP1 in brown adipose tissue (BAT), UCP2 in white adipose tissue (WAT), and UCP3 in skeletal muscle (MSL) mRNA by 1.3, 1.7, and 2.0-fold, respectively. In contrast, the expression of ob gene mRNA in WAT and serum leptin level in the T3-treated rats decreased by 0.5-fold of the controls. These results suggest that T3 may increase UCP family expression independent of leptin action.
Masaki
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Department of Internal Medicine 1, School of Medicine, Oita Medical University, Hasama, Oita, Japan. Masaki@oita-med.ac.jp
Yoshimatsu
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Sakata
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D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects*; D007473:Ion Channels; D020738:Leptin / Q000235:genetics / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D026901:Membrane Transport Proteins; D024101:Mitochondrial Proteins; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014284:Triiodothyronine / Q000494:pharmacology*; D000071256:Uncoupling Protein 1; D000071246:Uncoupling Protein 2; D000071247:Uncoupling Protein 3
D002352:Carrier Proteins; D007473:Ion Channels; D020738:Leptin; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D024101:Mitochondrial Proteins; D011506:Proteins; D012333:RNA, Messenger; C000604373:Ucp1 protein, rat; C000604391:Ucp2 protein, rat; C000604394:Ucp3 protein, rat; D000071256:Uncoupling Protein 1; D000071246:Uncoupling Protein 2; D000071247:Uncoupling Protein 3; D014284:Triiodothyronine