title: Comparison of three serological methods for detection of Lewis antigens on the surface of Helicobacter pylori.
abstract: Chemical studies have shown that lipopolysaccharide (LPS) from certain strains of Helicobacter pylori expresses the Lewis (Le) blood group antigens, predominantly Le(x) and/or Le(y). Serological methods have been used in a number of studies to assess the level of Le expression by H. pylori. However, serological and chemical analyses of a strain's LPS do not always agree. The purpose of this study was to evaluate and compare serological methods used to detect the expression of these antigens by H. pylori. A set of 34 Irish clinical isolates and three culture collection strains had their Le antigen expression assessed using three serological methods, serodot, Western blotting and enzyme-linked immunosorbent assay. A combination of all three methods was used to estimate Le(x) (59%) and Le(y) (94%) expression and this may allow a more accurate assessment of Le expression by isolates for pathogenesis studies.
authors.affiliation: Department of Microbiology, National University of Ireland, Galway.
authors.forename: S O
authors.initials: SO
authors.lastname: Hynes
authors.forename: A P
authors.initials: AP
authors.lastname: Moran
mesh_terms: D015153:Blotting, Western / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D016481:Helicobacter Infections / Q000382:microbiology; D016480:Helicobacter pylori / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D007983:Lewis Blood Group Antigens / Q000032:analysis*; D008070:Lipopolysaccharides / Q000032:analysis; D012015:Reference Standards
chemical_list: D007983:Lewis Blood Group Antigens; D008070:Lipopolysaccharides