Effect of Vitex rotundifolia on immediate-type allergic reaction.
We investigated the effect of aqueous extract of Vitex rotundifolia (L.) (Verbenaceae) fruits (VRFE) on the immediate-type allergic reactions in vivo and in vitro. VRFE (10(-4)-1.0 g/kg) dose-dependently inhibited systemic allergic reaction induced by compound 48/80. When VRFE was employed in a systemic allergic reaction test, the plasma histamine levels were reduced in a dose-dependent manner. VRFE (5x10(-1) and 1.0 g/kg) inhibited passive cutaneous anaphylaxis activated by anti-dinitrophenyl (DNP) IgE. VRFE (10(-3)-1.0 mg/ml) also dose-dependently inhibited the histamine release from the rat peritoneal mast cells (RPMC) by compound 48/80 or anti-DNP IgE. Moreover, VRFE (10(-3) mg/ml) had a significant inhibitory effect on anti-DNP IgE-induced tumor necrosis factor-alpha production from RPMC. These results suggest that VRFE may be beneficial in the regulation of immediate-type allergic reaction.
Shin
T Y
TY
College of Pharmacy, Woosuk University, Wanju, 565-701, Chonbuk, South Korea.
Kim
S H
SH
Lim
J P
JP
Suh
E S
ES
Jeong
H J
HJ
Kim
B D
BD
Park
E J
EJ
Hwang
W J
WJ
Rye
D G
DG
Baek
S H
SH
An
N H
NH
Kim
H M
HM
D000818:Animals; D006636:Histamine Release / Q000187:drug effects; D006969:Hypersensitivity, Immediate; D019686:Lamiaceae / Q000737:chemistry*; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha