Genetic screens for factors involved in the notum bristle loss of interspecific hybrids between Drosophila melanogaster and D. simulans.
Interspecific cross is a powerful means to uncover hidden within- and between-species variation in populations. One example is a bristle loss phenotype of hybrids between Drosophila melanogaster and D. simulans, although both the pure species have exactly the same pattern of bristle formation on the notum. There exists a large amount of genetic variability in the simulans populations with respect to the number of missing bristles in hybrids, and the variation is largely attributable to simulans X chromosomes. Using nine molecular markers, I screened the simulans X chromosome for genetic factors that were responsible for the differences between a pair of simulans lines with high (H) and low (L) missing bristle numbers. Together with duplication-rescue experiments, a single major quantitative locus was mapped to a 13F-14F region. Importantly, this region accounted for most of the differences between H and L lines in three other independent pairs, suggesting segregation of H and L alleles at the single locus in different populations. Moreover, a deficiency screening uncovered several regions with factors that potentially cause the hybrid bristle loss due to epistatic interactions with the other factors.
Takano-Shimizu
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Department of Population Genetics, National Institute of Genetics, Mishima, Shizuoka-ken 411-8540, Japan. totakano@lab.nig.ac.jp
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D017931:DNA Primers / Q000235:genetics; D004330:Drosophila / Q000033:anatomy & histology / Q000235:genetics*; D004331:Drosophila melanogaster / Q000033:anatomy & histology / Q000235:genetics*; D005260:Female; D020440:Gene Duplication; D017344:Genes, Insect; D005820:Genetic Testing; D014644:Genetic Variation; D006824:Hybridization, Genetic; D008297:Male; D010641:Phenotype; D019655:Quantitative Trait, Heritable; D013045:Species Specificity; D014960:X Chromosome / Q000235:genetics
D017931:DNA Primers