Structural characterisation and enzymic modification of the exopolysaccharide produced by Lactococcus lactis subsp. cremoris B891.
Lactococcus lactis subsp. cremoris B891 grown on whey permeate produced an exopolysaccharide containing D-Gal and D-Glc in a molar ratio of 2:3. The polysaccharide was partially O-acetylated. By means of HF solvolysis, O-deacetylation, enzymic modification, sugar linkage analysis and ID/2D NMR studies the exopolysaccharide was shown to be composed of repeating units with the following structure: [structure: see text].
van Casteren
W H
WH
Department of Food Technology and Nutritional Sciences, Wageningen University, The Netherlands.
de Waard
P
P
Dijkema
C
C
Schols
H A
HA
Voragen
A G
AG
D000107:Acetylation; D002240:Carbohydrate Sequence; D003903:Deuterium; D005690:Galactose; D005947:Glucose; D013294:Lactococcus lactis / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*
D011134:Polysaccharides; D003903:Deuterium; D005947:Glucose; D005690:Galactose