Molecular cloning of genomic DNA for fructose-1,6-bisphosphatase. From Aspergillus oryzae.
We have cloned and sequenced an Aspergillus oryzae genomic DNA fragment that encodes a fructose-1,6-bisphosphatase gene (fbpA) with the aim of studying transcriptional regulation mechanisms involved in basic metabolism. Expression of fbpA was repressed in the presence of glucose, but not in the presence of pyruvate or sodium acetate in the medium. The CreA and FacB element found in the fbpA 5'-flanking region may be important in fbpA regulation.
Sano
M
M
Department of Molecular Biology, National Institute of Bioscience and Humam-Techology, Ibaraki, Japan.
Nakajima
K
K
Takase
K
K
Yamamoto
M
M
Machida
M
M
D000595:Amino Acid Sequence; D001236:Aspergillus oryzae / Q000201:enzymology*; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000737:chemistry; D006597:Fructose-Bisphosphatase / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D012568:Schizosaccharomyces / Q000201:enzymology; D014512:Ureohydrolases / Q000235:genetics / Q000378:metabolism
D004271:DNA, Fungal; D006597:Fructose-Bisphosphatase; D014512:Ureohydrolases; C019944:creatinase