Pattern of angiogenic cytokine release from human vascular smooth muscle cells programmed by amino acid deprivation.
Vascular smooth muscle cells (VSMC) may be programmed by nutrient deprivation. We found that after 2 and 12 h exposure to 75% reduced amino acids, the release of basic fibroblast growth factor (bFGF) and transforming growth factor beta1 (TGF beta 1) from VSMC was significantly greater than that from cells maintained in control medium [2572.0 (546.3) vs 602.1 (241.7), P=0.001 and 16 028.0 (2192. 4) vs 13 027.3 (1233.5) pg/10(6)cells, P=0.022 respectively]. These differences were magnified after two passages of exposure for both bFGF (P=0.0001) and TGF beta 1 (P=0.0001). The stimulated release of VEGF by hypoxia and bFGF was unaffected. Amino acid deprivation of human VSMC is associated with a patterned release of angiogenic cytokines which could be relevant to the programmed changes in VSMC phenotype.
Pancholi
S
S
Centre for Diabetes and Cardiovascular Risk, Whittington Hospital, Clerkenwell Building, Royal Free And University College London Hospital Medical School, London N19 3UA, United Kingdom.
Earle
K A
KA
D000596:Amino Acids / Q000494:pharmacology*; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D016207:Cytokines / Q000096:biosynthesis*; D004730:Endothelium, Vascular / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis; D006801:Humans; D000860:Hypoxia; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D018919:Neovascularization, Physiologic; D010641:Phenotype; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000096:biosynthesis; D053773:Transforming Growth Factor beta1; D014471:Umbilical Veins / Q000187:drug effects / Q000378:metabolism
D000596:Amino Acids; D003470:Culture Media; D016207:Cytokines; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D016222:Fibroblast Growth Factor 2