title: Ovarian responses and oocyte recovery in prepubertal swamp buffalo (Bubalus bubalis) calves after FSH or PMSG treatment.
abstract: Stimulation of follicular growth was examined using two different gonadotropin treatments in 10 prepubertal swamp buffalo calves (8 to 12 mo old). Each calf received an ear implant consisting of 3 mg norgestromet and 5 mg estradiol valerate during hormonal treatment. Five calves were additionally administered FSH (24 mg, im) and, 2 mo later, PMSG (3,000 IU). The remaining 5 calves were first treated with PMSG followed by FSH. Ovarian responses to treatments were examined by laparotomy, 72 h after ear implant removal, and by the number of follicles (diameter > or = 0.8 cm) and corpora hemorrhagica present. Ovaries had more significant response to FSH than PMSG treatment (13.9+/-8.6 vs 5.9+/-3.3 follicles; P<0.01). Although the recovery rate tended to be lower for FSH treated (64%) than PMSG-treated (82%) animals, more oocytes/animal were harvested in the PMSG treatment (8.3+/-5.0 vs 4.6+/-3.2, respectively). The immature oocytes (n = 38) were cultured for 24 to 25 h in maturation medium (TCM-199 NaHCO3+10% fetal calf serum [FCS] in 5%CO2 in air at 39 degrees C). Oocyte maturation was assessed after fixation and staining with aceto orcein. The in vitro maturation rate was 52.6% (20/38). This study shows the possibility of harvesting oocytes from prepubertal swamp buffalo calves and maturing the oocyte in vitro.
authors.affiliation: Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
authors.forename: M
authors.initials: M
authors.lastname: Techakumphu
authors.forename: C
authors.initials: C
authors.lastname: Lohachit
authors.forename: W
authors.initials: W
authors.lastname: Tantasuparak
authors.forename: C
authors.initials: C
authors.lastname: Intaramongkol
authors.forename: S
authors.initials: S
authors.lastname: Intaramongkol
mesh_terms: D000818:Animals; D002020:Buffaloes / Q000502:physiology*; D018929:Cell Culture Techniques; D004396:Coloring Agents / Q000737:chemistry; D004958:Estradiol / Q000008:administration & dosage / Q000494:pharmacology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000008:administration & dosage / Q000494:pharmacology*; D006064:Gonadotropins, Equine / Q000008:administration & dosage / Q000494:pharmacology*; D007813:Laparotomy / Q000662:veterinary; D008858:Microscopy, Phase-Contrast / Q000662:veterinary; D009865:Oocytes / Q000254:growth & development / Q000502:physiology*; D006080:Ovarian Follicle / Q000502:physiology*; D010078:Oxazines / Q000737:chemistry; D010865:Pilot Projects; D011282:Pregnenediones / Q000008:administration & dosage / Q000494:pharmacology; D011897:Random Allocation
chemical_list: D004396:Coloring Agents; D006064:Gonadotropins, Equine; D010078:Oxazines; D011282:Pregnenediones; C018437:aceto-orcein; C100284:norgestomet; D004958:Estradiol; D005640:Follicle Stimulating Hormone