Identification of diverse nerve growth factor-regulated genes by serial analysis of gene expression (SAGE) profiling.
Neurotrophic factors such as nerve growth factor (NGF) promote a wide variety of responses in neurons, including differentiation, survival, plasticity, and repair. Such actions often require changes in gene expression. To identify the regulated genes and thereby to more fully understand the NGF mechanism, we carried out serial analysis of gene expression (SAGE) profiling of transcripts derived from rat PC12 cells before and after NGF-promoted neuronal differentiation. Multiple criteria supported the reliability of the profile. Approximately 157,000 SAGE tags were analyzed, representing at least 21,000 unique transcripts. Of these, nearly 800 were regulated by 6-fold or more in response to NGF. Approximately 150 of the regulated transcripts have been matched to named genes, the majority of which were not previously known to be NGF-responsive. Functional categorization of the regulated genes provides insight into the complex, integrated mechanism by which NGF promotes its multiple actions. It is anticipated that as genomic sequence information accrues the data derived here will continue to provide information about neurotrophic factor mechanisms.
Angelastro
J M
JM
Department of Pathology, Center for Neurobiology and Behavior and Taub Institute, Columbia University College of Physicians and Surgeons, 630 West 168th Street, New York, NY 10032, USA.
Klimaschewski
L
L
Tang
S
S
Vitolo
O V
OV
Weissman
T A
TA
Donlin
L T
LT
Shelanski
M L
ML
Greene
L A
LA
D048948:14-3-3 Proteins; D000818:Animals; D001483:Base Sequence; D002455:Cell Division; D002462:Cell Membrane / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism; D017931:DNA Primers; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology*; D005982:Glutathione Transferase / Q000235:genetics; D020932:Nerve Growth Factor / Q000502:physiology*; D016716:PC12 Cells; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D014157:Transcription Factors / Q000235:genetics; D014446:Tyrosine 3-Monooxygenase
D048948:14-3-3 Proteins; D017931:DNA Primers; D011506:Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; D020932:Nerve Growth Factor; D014446:Tyrosine 3-Monooxygenase; D005982:Glutathione Transferase