Preparation of radioactive aldehyde-containing phosphatidylcholine.
Short-chain, aldehyde-containing phosphatidylcholine (PC), formed during the oxidation of PC, is thought to be involved in cellular responses in atherosclerosis and inflammation. Here we report a convenient procedure for a small-scale preparation of aldehyde-containing PC. PC containing an unsaturated fatty acyl chain was treated with osmium tetroxide followed by sodium periodate at room temperature. The reaction product was purified by TLC. This preparation showed a single peak on reverse-phase HPLC, and its identity was confirmed by fast atom bombardment mass spectrometry. This procedure does not require special equipment and is easily applicable for preparation of radioactive materials.
Itabe
H
H
Department of Microbiology and Molecular Pathology, Faculty of Pharmaceutical Sciences, Teikyo University, Sagamiko, Tsukui, Kanagawa, 199-0195, Japan. h-itabe@pharm.teikyo-u.ac.jp
Suzuki
K
K
Hosoya
R
R
Mori
M
M
Higashi
Y
Y
Fujimoto
Y
Y
Takano
T
T
D000447:Aldehydes / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D010713:Phosphatidylcholines / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment
D000447:Aldehydes; D010713:Phosphatidylcholines