The influence of photoperiodic growth condition on isolation of RNA from strawberry (Fragaria x ananassa Duch.) tissue.
Purification of high-quality RNA from different strawberry tissues is often affected by the presence of high levels of contamination by polysaccharides and phenolic compounds. With the protocol detailed here we describe for the first time total RNA purification from petiole tissue. Treating the plants used as source of material with short-daylight regime prior the extraction we are able to obtain RNA suitable for further applications such as in vitro translation, RT-PCR, and RNA blot analysis. The yield of total RNA extraction is significantly enhanced when tissue from plants grown under short-day photoperiodic condition is used compared with that taken from plants grown under long day photoperiod.
Mazzara
M
M
Dipartimento di Biotecnologie Agrarie ed Ambientali, Facoltà di Agraria, University of Ancona, Italy. Mazzara@popcsi.unian.it
James
D J
DJ
D004587:Electrophoresis, Agar Gel; D017440:Photoperiod; D018515:Plant Leaves / Q000737:chemistry / Q000235:genetics*; D018749:RNA, Plant / Q000302:isolation & purification*; D019685:Rosales / Q000033:anatomy & histology / Q000737:chemistry / Q000235:genetics*
D018749:RNA, Plant