Human monocytes express CD163, which is upregulated by IL-10 and identical to p155.
CD163 is a glucocorticoid-inducible member of the scavenger receptor cysteine-rich family of proteins. Previous reports have indicated that CD163 is highly expressed on human macrophages, but found on less than 50% of peripheral blood monocytes. We now show that >99% of all CD14 positive monocytes express CD163 and that monocyte derived dendritic cells express low levels of CD163. We also show that IL-10, like glucocorticoids, induces high CD163 expression on cultured human monocytes. Glucocorticoid induced CD163 expression was not inhibited by anti-IL-10 and was additive with IL-10 treatment, suggesting that glucocorticoids increase CD163 expression by an IL-10 independent mechanism. Other anti-inflammatory cytokines (IL-4 and IL-13) did not increase CD163 expression. In addition, we show that p155 (a previously identified monocyte/macrophage marker of unknown function) shares identity with CD163. Western blots and flow cytometric analysis of HEK 293 cells transfected with the cDNA for CD163 were positive when probed with either mAb RM3/1 (which recognizes CD163) or Mac 2-48 (which defines p155).
Sulahian
T H
TH
Department of Physiology, Dartmouth Medical School, Lebanon, NH, 03756, USA.
Högger
P
P
Wahner
A E
AE
Wardwell
K
K
Goulding
N J
NJ
Sorg
C
C
Droste
A
A
Stehling
M
M
Wallace
P K
PK
Morganelli
P M
PM
Guyre
P M
PM
D000818:Animals; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic / Q000096:biosynthesis*; D015152:Blotting, Northern; D015153:Blotting, Western; D002460:Cell Line; D016207:Cytokines / Q000494:pharmacology; D018076:DNA, Complementary / Q000378:metabolism; D003713:Dendritic Cells / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D005938:Glucocorticoids / Q000494:pharmacology; D006801:Humans; D016753:Interleukin-10 / Q000494:pharmacology*; D018793:Interleukin-13 / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D009000:Monocytes / Q000378:metabolism*; D010587:Phagocytosis; D012333:RNA, Messenger / Q000378:metabolism; D011956:Receptors, Cell Surface; D014158:Transcription, Genetic / Q000187:drug effects; D014162:Transfection; D015854:Up-Regulation; D000098902:CD163 Antigen
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D000098902:CD163 Antigen; D016207:Cytokines; D018076:DNA, Complementary; D005938:Glucocorticoids; D018793:Interleukin-13; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D016753:Interleukin-10; D015847:Interleukin-4; D003907:Dexamethasone