[Enterocin-35, a bacteriocin with activity against Listeria monocytogenes. Possible use in the food industry].
The in vitro inhibitory activity of enterocin-35 produced by Enterococcus faecium CRL 35, was studied against Listeria monocytogenes, isolated from seafoods. Optimal growth conditions of the enterocin-35 producing strain, for higher bacteriocin production and improve the extraction and purification of these peptides, were applied. A crude extract of enterocin-35 was assayed in a frozen seafood artificially contaminated with Listeria monocytogenes isolate, simulating at laboratory scale an eventual application of this biopreservant in a routine production process at factory level. The feasibility of biopreservation of seafoods by means of bacteriocins is proposed and discussed.
Concha
R
R
Departamento de Microbiología, Facultad de Ciencias Biológicas, Universidad de Concepción, Chile. rconcha@udec.cl
Farías
M E
ME
Kümmerlin
R
R
Sesma
F
F
D001430:Bacteriocins / Q000302:isolation & purification / Q000494:pharmacology*; D015925:Cryopreservation; D016984:Enterococcus faecium / Q000378:metabolism*; D005240:Feasibility Studies; D005506:Food Contamination; D019649:Food Industry; D005516:Food Microbiology; D005519:Food Preservation; D005520:Food Preservatives / Q000494:pharmacology*; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D017747:Seafood / Q000382:microbiology*
D001430:Bacteriocins; D005520:Food Preservatives; C121783:enterocin CRL35