Simultaneous detection of different glycosidase activities by 19F NMR spectroscopy.
A fast method for the simultaneous detection of different glycosidolytic activities in commercially available enzyme preparations and crude culture filtrates was found in using, as substrate, a mixture of different glycosyl fluorides and 19F NMR spectroscopy as a screening technique. Accompanying studies regarding the hydrolytic stability of these fluorides in various buffer systems, as well as conditions of their long-term storage, were carried out. A simple procedure for the preparation of beta-D-mannopyranosyl fluoride in gram quantities is given.
Albert
M
M
Institute of Organic Chemistry, Technical University, Graz, Austria.
Repetschnigg
W
W
Ortner
J
J
Gomes
J
J
Paul
B J
BJ
Illaszewicz
C
C
Weber
H
H
Steiner
W
W
Dax
K
K
D005461:Fluorine / Q000737:chemistry / Q000378:metabolism; D005462:Fluorine Radioisotopes; D006026:Glycoside Hydrolases / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods
D005462:Fluorine Radioisotopes; D005461:Fluorine; D006026:Glycoside Hydrolases