title: Coupling high-pressure MALDI with ion mobility/orthogonal time-of-flight mass spectrometry.
abstract: A new ion mobility/time-of-flight mass spectrometer employing a high-pressure MALDI source has been designed and tested. The prototype instrument operates at a source/drift cell pressure of 1-10 Torr helium, resulting in a mobility resolution of approximately 25. A small time-of-flight mass spectrometer (20 cm) with a mass resolution of up to 200 has been attached to the drift cell to identify (in terms of mass-to-charge ratio) the separated ions. A simple tripeptide mixture has been separated in the drift tube and mass identified as singly protonated species. The ability to separate peptide mixtures, e.g., tryptic digest of a protein, is illustrated and compared to results obtained on a high-vacuum time-of-flight instrument.
authors.affiliation: Department of Chemistry, Texas A&M University, College Station 77843, USA.
authors.forename: K J
authors.initials: KJ
authors.lastname: Gillig
authors.forename: B
authors.initials: B
authors.lastname: Ruotolo
authors.forename: E G
authors.initials: EG
authors.lastname: Stone
authors.forename: D H
authors.initials: DH
authors.lastname: Russell
authors.forename: K
authors.initials: K
authors.lastname: Fuhrer
authors.forename: M
authors.initials: M
authors.lastname: Gonin
authors.forename: A J
authors.initials: AJ
authors.lastname: Schultz
mesh_terms: D000595:Amino Acid Sequence; D013058:Mass Spectrometry / Q000295:instrumentation*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D010455:Peptides