Effect of colon carcinoma cell supernatants on cytokine production and phagocytic capacity.
The ability of colon carcinoma cells to produce IL-1 beta, IL-6 and TNF alpha, and the effect of tumor cell supernatants (sups) on the capacity of peripheral blood mononuclear cells to produce these three cytokines was examined. In addition, the effect of colon carcinoma cell sups on the engulfing capacity of phagocytic cells was detected. The results showed that IL-1 beta, IL-6 and TNF alpha levels were significantly higher in tumor cell sups compared with those of autologous colon mucosal cells obtained from healthy tissue. Tumor cell sups caused a decrease in both phagocytic capacity, and the number of latex particles engulfed by each individual cell.
Salman
H
H
Department of Medicine C, Rabin Medical Center, Golda Campus, Petah Tiqva, Israel.
Bergman
M
M
Bessler
H
H
Wolloch
Y
Y
Punsky
I
I
Djaldetti
M
M
D017077:Culture Media, Conditioned / Q000494:pharmacology*; D016207:Cytokines / Q000096:biosynthesis / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D007840:Latex / Q000493:pharmacokinetics; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010316:Particle Size; D010587:Phagocytosis / Q000187:drug effects*; D013481:Superoxides / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D017077:Culture Media, Conditioned; D016207:Cytokines; D007375:Interleukin-1; D015850:Interleukin-6; D007840:Latex; D014409:Tumor Necrosis Factor-alpha; D013481:Superoxides