Efficient synthesis of a sialyl T-antigen-linked glycopeptide by the chemoenzymatic method.
A sialyl T-antigen-linked tetrapeptide was prepared by the combined method of chemical synthesis and enzymatic synthesis. The GalNAc-linked peptide was first obtained by using a commercial peptide synthesizer, and then a galactose residue was attached with beta-(1-->3)-linkage by transglycosylating with a recombinant beta-galactosidase from Bacillus circulans. The sialic acid residue was then combined by alpha-(2-->3)-linkage with sialytransferase from rat liver.
Ajisaka
K
K
Meiji Institute of Health Science, Meiji Milk Products Co. Ltd, Odawara, Japan. kajisaka@mbc.sphere.ne.jp
Miyasato
M
M
D000818:Animals; D001407:Bacillus / Q000201:enzymology*; D002236:Carbohydrate Conformation; D002851:Chromatography, High Pressure Liquid; D005690:Galactose / Q000378:metabolism; D006020:Glycopeptides / Q000138:chemical synthesis*; D006031:Glycosylation; D008099:Liver / Q000201:enzymology; D009077:Mucins / Q000138:chemical synthesis*; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
D006020:Glycopeptides; D009077:Mucins; D011994:Recombinant Proteins; C087769:sialosyl-T antigen; D001616:beta-Galactosidase; D005690:Galactose