title: DNA encoding the attachment (G) or fusion (F) protein of respiratory syncytial virus induces protection in the absence of pulmonary inflammation.
abstract: Significant protection against respiratory syncytial virus (RSV) infection was induced in mice vaccinated intramuscularly (i.m.) with DNA encoding the F or G protein of RSV. The amounts of IgG1 of IgG2a antibodies in mice immunized with DNA-G alone were similar. However, the antibody response in mice co-immunized with DNA-G and DNA encoding IL-4 (DNA-IL-4) was strongly biased towards IgG1. In contrast, the antibody response in mice co-immunized with DNA-G and DNA-IL-2, -IL-12 or-IFN-gamma was biased towards IgG2a. Mice vaccinated with DNA-F either alone or in combination with DNA encoding cytokines developed a predominant RSV-specific IgG2a response, which was most pronounced in mice co-immunized with DNA-F and DNA-IL-12 or -IFN-gamma. Vaccinated mice developed only a slightly enhanced pulmonary inflammatory response following RSV challenge. More significantly, and in contrast to mice scarified with recombinant vaccinia virus expressing the G protein, mice vaccinated i.m. with DNA-G did not develop pulmonary eosinophilia, even when the immune response was biased towards a Th2 response by co-administration of DNA-IL-4.
authors.affiliation: Institute for Animal Health, Compton, Newbury, Berkshire RG20 7NN , UK1.
authors.forename: Gary P
authors.initials: GP
authors.lastname: Bembridge
authors.affiliation: Centro Nacional de Biologia Fundamental, Instituto de Salud Carlos III, Majadahonda, 28220 Madrid, Spain2.
authors.forename: Nuria
authors.initials: N
authors.lastname: Rodriguez
authors.affiliation: Centro Nacional de Biologia Fundamental, Instituto de Salud Carlos III, Majadahonda, 28220 Madrid, Spain2.
authors.forename: Regina
authors.initials: R
authors.lastname: Garcia-Beato
authors.affiliation: NIBSC, Blanche Lane, South Mimms, Potters Bar, Hertfordshire EN6 3QG, UK3.
authors.forename: Carolyn
authors.initials: C
authors.lastname: Nicolson
authors.affiliation: Centro Nacional de Biologia Fundamental, Instituto de Salud Carlos III, Majadahonda, 28220 Madrid, Spain2.
authors.forename: Jose A
authors.initials: JA
authors.lastname: Melero
authors.affiliation: Institute for Animal Health, Compton, Newbury, Berkshire RG20 7NN , UK1.
authors.forename: Geraldine
authors.initials: G
authors.lastname: Taylor
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000956:Antigens, Viral / Q000235:genetics*; D004195:Disease Models, Animal; D007371:Interferon-gamma / Q000494:pharmacology; D018664:Interleukin-12 / Q000494:pharmacology; D051379:Mice; D011024:Pneumonia, Viral / Q000517:prevention & control* / Q000821:virology*; D011657:Pulmonary Eosinophilia / Q000517:prevention & control / Q000821:virology; D018357:Respiratory Syncytial Virus Infections / Q000517:prevention & control*; D012136:Respiratory Syncytial Viruses / Q000235:genetics*; D018418:Th2 Cells / Q000276:immunology / Q000821:virology; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins / Q000235:genetics*; D014760:Viral Fusion Proteins / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; D014764:Viral Proteins; D018664:Interleukin-12; D007371:Interferon-gamma