Study of the effect of the administration of Cd(II), cysteine, methionine, and Cd(II) together with cysteine or methionine on the conversion of xanthine dehydrogenase into xanthine oxidase.
Cadmium is known as to be a potent pulmonary carcinogen to human beings and to induce prostate tumor. The sequestration of cadmium, an extremely toxic element to living cells, which is performed by biological ligands such as amino acids, peptides, proteins or enzymes is important to minimize its participation in such deleterious processes. The synthesis of metallothionein is induced by a wide range of metals, in which cadmium is a particularly potent inducer. This protein is usually associated with cadmium exposure in man. Because metallothioneins may act as a detoxification agent for cadmium and chelation involves sulfur donor atoms, we administered only cadmium, cysteine, or methionine to rats and also each of these S-amino acids together with cadmium and measured the production of superoxide radicals derived from the conversion of xanthine dehydrogenase to xanthine oxidase. It could be seen in this work that the presence of cadmium enhances this conversion. However, its inoculation with cysteine or methionine almost completely diminishes this effect and this can be the result of the fact that these amino acids complex Cd(II). Thus, these compounds can be a model of the action of metallothionein, removing cadmium from circulation and preventing its deleterious effect.
Esteves
A C
AC
Department of Chemistry, Pontifícia Universidade Católica do Rio de Janeiro, Rio de Janeiro, Brazil.
Felcman
J
J
D000818:Animals; D002104:Cadmium / Q000494:pharmacology*; D003545:Cysteine / Q000494:pharmacology*; D005609:Free Radicals; D008297:Male; D008715:Methionine / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013481:Superoxides; D013997:Time Factors; D014968:Xanthine Dehydrogenase / Q000378:metabolism*; D014969:Xanthine Oxidase / Q000378:metabolism*
D005609:Free Radicals; D002104:Cadmium; D013481:Superoxides; D008715:Methionine; D014968:Xanthine Dehydrogenase; D014969:Xanthine Oxidase; D003545:Cysteine