title: House dust mite-specific IgE antibodies in induced sputum are associated with sputum eosinophilia in mite-sensitive asthmatics.
abstract: BACKGROUND
  Although allergen-specific IgE antibodies have been considered to play an important role in the pathogenesis of atopic asthma, the role of IgE antibodies in the development of airway inflammation is not well defined.
  
  
  OBJECTIVE
  To evaluate the association between allergen-specific IgE antibodies and inflammation of the asthmatic airway.
  
  
  METHODS
  We measured house dust mite (HDM; Dermatophagoides farinae)-specific IgE antibodies in both serum and induced sputum from 16 HDM-sensitive asthmatic patients, and evaluated their association with sputum eosinophilia and eosinophil cationic protein (ECP) levels in induced sputum.
  
  
  RESULTS
  Levels of HDM-specific IgE antibodies in induced sputum were significantly higher in asthmatic patients than in controls (P < .01). In asthmatic patients, levels of HDM-specific IgE antibodies were significantly higher in induced sputum samples with eosinophilia (sputum eosinophil count > or = 5% of 200 counted non-squamous cells) than in those without eosinophilia (P < .05). There were no significant differences in serum levels of HDM-specific IgE antibodies between asthmatic patients with sputum eosinophilia and asthmatic patients without sputum eosinophilia. In asthmatic patients, sputum ECP levels were significantly correlated with levels of HDM-specific IgE antibodies (r = 0.60, P = .01) in induced sputum but not with those in serum.
  
  
  CONCLUSIONS
  We conclude that allergen-specific IgE antibodies in induced sputum from atopic asthmatics are associated with sputum eosinophilia. This result suggests that IgE-dependent mechanisms are involved in eosinophilic inflammation of the airway in atopic asthmatics.
authors.affiliation: Department of Allergy and Clinical Immunology, Ajou University Hospital, Suwon, Korea.
authors.forename: D H
authors.initials: DH
authors.lastname: Nahm
authors.forename: H Y
authors.initials: HY
authors.lastname: Kim
authors.forename: H S
authors.initials: HS
authors.lastname: Park
mesh_terms: D000485:Allergens / Q000276:immunology; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis* / Q000097:blood / Q000276:immunology; D000918:Antibody Specificity; D039741:Antigens, Dermatophagoides; D001249:Asthma / Q000150:complications* / Q000276:immunology*; D001798:Blood Proteins / Q000378:metabolism; D047091:Eosinophil Granule Proteins; D004802:Eosinophilia / Q000276:immunology*; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D018836:Inflammation Mediators / Q000378:metabolism; D008925:Mites / Q000276:immunology; D012260:Ribonucleases; D013183:Sputum / Q000737:chemistry / Q000276:immunology*
chemical_list: D000485:Allergens; D000888:Antibodies, Anti-Idiotypic; D039741:Antigens, Dermatophagoides; D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D006023:Glycoproteins; D018836:Inflammation Mediators; C026408:anti-IgE antibodies; D007073:Immunoglobulin E; D012260:Ribonucleases