title: p53 protein expression and proliferative activity in ductal breast carcinomas.
abstract: The immunocytochemical expression of p53 protein and Ki-67 labelling index in tumour cells of 100 ductal breast carcinomas of different histological grade and stage was evaluated in cytological material. In order to investigate p53 expression and Ki-67 expression an avidin-extravidin immunocytochemical technique was applied to imprints. Monoclonal antibody (MoAb) DO-p53 and proliferating cell monoclonal antibody were used as primary antibodies. A statistically significant difference was observed between p53 protein expression and grade of malignancy and clinical stage (P = 0.001, P < 0.001, respectively). A statistically significant difference was also observed between Ki-67 LI and histological grade and stage of the tumours (P < 0.001, P < 0.001 correspondingly). A correlation was observed between p53 protein expression and Ki-67 LI (P < 0.001). The immunocytochemical study of p53 protein and Ki-67 expression in cytological material represents a simple method which can be applied in routine cytological laboratories for the investigation of potential malignancy of ductal breast cancer.
authors.affiliation: Department of Pathology, Cytopathology Unit, Medical School, University of Athens, Greece.
authors.forename: A
authors.initials: A
authors.lastname: Ioakim-Liossi
authors.forename: M
authors.initials: M
authors.lastname: Safioleas
authors.forename: P
authors.initials: P
authors.lastname: Karakitsos
authors.forename: P
authors.initials: P
authors.lastname: Athanassiadou
authors.forename: P
authors.initials: P
authors.lastname: Athanassiades
authors.forename: J
authors.initials: J
authors.lastname: Gogas
authors.forename: L
authors.initials: L
authors.lastname: Nakopoulou
mesh_terms: D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology*; D018270:Carcinoma, Ductal, Breast / Q000378:metabolism* / Q000473:pathology*; D002455:Cell Division; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism*; D008875:Middle Aged; D009367:Neoplasm Staging; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D019394:Ki-67 Antigen; D016159:Tumor Suppressor Protein p53