Differential effects of fluoride and insulin-like growth factor I on sodium-dependent alanine and phosphate transport in a human osteoblast-like cell line.
The effects of fluoride and insulin-like growth factor (IGF)-I on sodium-dependent (Na(d)) alanine and phosphate (Pi) transport were compared in a human osteosarcoma cell line, SAOS-2/B-10. Fluoride stimulated Na(d) alanine but not Pi uptake in a dose-dependent manner, whereas IGF-I stimulated both alanine and Na(d)Pi transport. IGF-I and low concentrations of fluoride stimulated Na(d) alanine transport rapidly. Genistein, an inhibitor of tyrosine kinase blocked IGF-I- but not fluoride-stimulated Na(d) alanine transport. The effects of fluoride and IGF-I were additive and not associated with corresponding changes in cell number or protein content. In conclusion, low concentrations of fluoride rapidly and selectively stimulate Na(d) alanine transport in SAOS-2 cells.
Veldman
C M
CM
Department of Internal Medicine, University Hospital, Zürich, Switzerland. endosam@usz.unizh.ch
Schmid
C
C
D000409:Alanine / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D001859:Bone Neoplasms / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005459:Fluorides / Q000494:pharmacology*; D019833:Genistein / Q000494:pharmacology; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D012516:Osteosarcoma / Q000473:pathology; D010710:Phosphates / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D012964:Sodium / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
D004791:Enzyme Inhibitors; D010710:Phosphates; D007334:Insulin-Like Growth Factor I; D012964:Sodium; D019833:Genistein; D011505:Protein-Tyrosine Kinases; D000409:Alanine; D005459:Fluorides