Effect of oral CDP-choline on plasma choline and uridine levels in humans.
Twelve mildly hypertensive but otherwise normal fasting subjects received each of four treatments in random order: CDP-choline (citicoline; 500, 2000, and 4000 mg) or a placebo orally at 8:00 a.m. on four different treatment days. Eleven plasma samples from each subject, obtained just prior to treatment (8:00 a.m.) and 1-12 hr thereafter, were assayed for choline, cytidine, and uridine. Fasting terminated at noon with consumption of a light lunch that contained about 100 mg choline. Plasma choline exhibited dose-related increases in peak values and areas under the curves (AUCs), remaining significantly elevated, after each of the three doses, for 5, 8, and 10 hr, respectively. Plasma uridine was elevated significantly for 5-6 hr after all three doses, increasing by as much as 70-90% after the 500 mg dose, and by 100-120% after the 2000 mg dose. No further increase was noted when the dose was raised from 2000 to 4000 mg. Plasma cytidine was not reliably detectable, since it was less than twice blank, or less than 100 nM, at all of the doses. Uridine is known to enter the brain and to be converted to UTP; moreover, we found that uridine was converted directly to CTP in neuron-derived PC-12 cells. Hence, it seems likely that the circulating substrates through which oral citicoline increases membrane phosphatide synthesis in the brains of humans involve uridine and choline, and not cytidine and choline as in rats.
Wurtman
R J
RJ
Department of Brain & Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. dick@mit.edu
Regan
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Ulus
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Yu
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D000284:Administration, Oral; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D002794:Choline / Q000097:blood*; D003562:Cytidine / Q000097:blood; D003566:Cytidine Diphosphate Choline / Q000097:blood* / Q000378:metabolism; D003570:Cytidine Triphosphate / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018697:Nootropic Agents / Q000097:blood* / Q000378:metabolism; D016716:PC12 Cells; D051381:Rats; D014529:Uridine / Q000097:blood* / Q000378:metabolism; D014544:Uridine Triphosphate / Q000032:analysis
D018697:Nootropic Agents; D003566:Cytidine Diphosphate Choline; D003562:Cytidine; D003570:Cytidine Triphosphate; D002794:Choline; D014544:Uridine Triphosphate; D014529:Uridine