title: Analytical techniques used for monitoring the biodegradation of fluorinated compounds in waste streams from pharmaceutical production.
abstract: During the assessment of the environmental impact of new pharmaceutical processes the selection and testing of suitable environmental treatment technologies is carried out. A large component of process waste stream treatment practice is aerobic biotreatment in wastewater treatment plants, as it is cost effective and generally more environmentally friendly than harsher chemical/physical treatments. Pharmaceutical syntheses use a range of halogenated compounds (either as reagents, solvents or intermediates) which pose particular challenges to microbial degradation. This is especially so for some fluorinated compounds due to the resilience to enzymatic cleavage of the C-F bond in some cases. The data presented here were obtained from a case study involving the monitoring of the biodegradation of 4-fluorocinnamic acid by means of a range of chromatographic techniques. These methods were used to monitor not only the disappearance of the compound but also the formation of degradation products in order to confirm mineralisation. In addition mass spectrometry was used to elucidate the metabolic pathway.
authors.affiliation: Environmental Research Laboratory, Analytical Sciences, SmithKline Beecham Pharmaceuticals, Harlow, Essex, UK.
authors.forename: A P
authors.initials: AP
authors.lastname: New
authors.forename: L M
authors.initials: LM
authors.lastname: Freitas dos Santos
authors.forename: G
authors.initials: G
authors.lastname: Lo Biundo
authors.forename: A
authors.initials: A
authors.lastname: Spicq
mesh_terms: D001673:Biodegradation, Environmental; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D019411:Clinical Laboratory Techniques; D004345:Drug Industry; D017611:Fluorine Compounds / Q000032:analysis / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D012722:Sewage / Q000032:analysis; D014867:Water / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism*
chemical_list: D017611:Fluorine Compounds; D012722:Sewage; D014874:Water Pollutants, Chemical; D014867:Water