[A new electrometric approach to studying specific biomolecular complexes].
In the article the results of the influence of some types of biological molecules and their specific immune complexes on the volt-amperic characteristics of surface-barrier contact structures with the super thick metal film are presented. Moreover, the possibility to develop on this basis a simple instrumental method for separate registration of initial components and products of their interaction is discussed. It was shown that the volt-amperic characteristics of surface-barrier structures of Ni-Si changed at the deposition of myoglobin and its specific monoclonal antibodies. These structures more essentially reacted to the formed presence of specific immune complex and, in particular, to the direct formation of this complex on the surface at the subsequent use of the initial immune components in comparison with their separate presence. For all investigated types of biological molecules and their specific complexes we found optimal thickness of metal film when changes of volt-amperic characteristics of system achieved maximum level. It was concluded that this electrometrical method was suitable for the express and separation free registration of specific immune complex.
Il'chenko
V V
VV
Kyiv National Taras Shevchenko University, Ukraine.
Starodub
V N
VN
Goraĭchuk
I P
IP
Nakhodkin
A T
AT
Starodub
N F
NF
D000911:Antibodies, Monoclonal / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods*; D009211:Myoglobin / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D009211:Myoglobin