Preliminary communication. MRNA expression of MT1-MMP, MMP-9, cathepsin K, and TRAP in highly enriched osteoclasts cultured on several matrix proteins and ivory surfaces.
We demonstrated that the transcriptional expression of MT1-MMP, MMP-9, cathepsin K, and TRAP in highly enriched osteoclasts were regulated by different matrix proteins that bind to integrin on osteoclast, such as collagen type I (CoI), fibronectin (FN), vitronectin (VN), osteopontin (OPN), and ivory. Results suggested that the OPN-integrin alphavbeta3 binding plays a more important role than CoI-alpha2beta1 binding in the regulation of osteoclast activity.
Uemura
T
T
National Institute for Advanced Interdisciplinary Research, Tsukuba Research Center, Ibaraki, Japan. uemura@nair.go.jp
Liu
Y K
YK
Kuboki
Y
Y
D000818:Animals; D001426:Bacterial Proteins; D056657:Cathepsin K; D002403:Cathepsins / Q000096:biosynthesis* / Q000235:genetics; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis* / Q000235:genetics; D053504:Matrix Metalloproteinases, Membrane-Associated; D008666:Metalloendopeptidases / Q000096:biosynthesis* / Q000235:genetics; D010010:Osteoclasts / Q000201:enzymology*; D053495:Osteopontin; D012333:RNA, Messenger / Q000096:biosynthesis*; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D011817:Rabbits; D019030:Receptors, Vitronectin / Q000378:metabolism; D012795:Sialoglycoproteins / Q000378:metabolism; D013499:Surface Properties; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D014158:Transcription, Genetic; D019096:Vitronectin / Q000378:metabolism
D001426:Bacterial Proteins; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; C079092:MtrB protein, Bacteria; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D019030:Receptors, Vitronectin; D012795:Sialoglycoproteins; D014157:Transcription Factors; D019096:Vitronectin; D053495:Osteopontin; D003094:Collagen; D002403:Cathepsins; D056657:Cathepsin K; D053504:Matrix Metalloproteinases, Membrane-Associated; D008666:Metalloendopeptidases; D020780:Matrix Metalloproteinase 9