Monoclonal antibodies against topoisomerase I suppressed DNA relaxation and HIV-1 cDNA synthesis.
Human immunodeficiency virus type 1 (HIV-1) virion is known to carry a number of cellular components including cellular topoisomerase I. Previously, we have demonstrated that topoisomerase I enhances HIV-1 cDNA synthesis in reverse transcription (RT) assays in vitro. In the present study, we have produced six monoclonal antibodies (MAbs) against human topoisomerase I. The MAbs suppressed nicking/closing of supercoiled DNA and cDNA synthesis in an endogenous reverse transcription (ERT) assay using a detergent-disrupted HIV-1 virion. Thus, the results suggest that topoisomerase I plays an important role in RNA-directed DNA polymerization.
Takahashi
H
H
Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan. htakahas@nih.go.jp
Iwata
T
T
Kitagawa
Y
Y
Shoya
Y
Y
Takahashi
R H
RH
Nagashima
K
K
Kurata
T
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D000918:Antibody Specificity / Q000276:immunology; D015139:Blotting, Southern; D002460:Cell Line; D004261:DNA Replication / Q000187:drug effects*; D004264:DNA Topoisomerases, Type I / Q000276:immunology* / Q000302:isolation & purification; D018076:DNA, Complementary / Q000096:biosynthesis*; D004279:DNA, Viral / Q000187:drug effects*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015497:HIV-1 / Q000187:drug effects* / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D012367:RNA, Viral / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D014779:Virus Replication
D000911:Antibodies, Monoclonal; D018076:DNA, Complementary; D004279:DNA, Viral; D012367:RNA, Viral; D004264:DNA Topoisomerases, Type I