title: Transforming growth factor beta1 ameliorates intestinal epithelial barrier disruption by Cryptosporidium parvum in vitro in the absence of mucosal T lymphocytes.
abstract: Exposure to oocysts of the protozoan Cryptosporidium parvum causes intestinal epithelial cell dysfunction in vivo and in vitro, but effective means by which mucosal injury might be prevented remain unclear. We examined the ability of transforming growth factor beta1 (TGF-beta1)-a cytokine synthesized and released by cells in the intestine-to preserve the barrier function of human colonic epithelia when challenged with C. parvum oocysts and then studied the mechanisms involved. Epithelial barrier function was monitored electrophysiologically, receptors for TGF-beta1 were localized by confocal microscopy, and TGF-beta1-induced protein kinase C activation was detected intracellularly by translocation of its alpha isozyme. TGF-beta1 alone enhanced intestinal epithelial barrier function, while exposure to C. parvum oocysts (> or =10(5)/monolayer) markedly reduced barrier function to < or =40% of that of the control. When epithelial monolayers were pretreated with TGF-beta1 at 5.0 ng/ml, the barrier-disrupting effect of C. parvum oocysts was almost completely abrogated for 96 h. Further investigation showed that (i) the RI and RII receptors for TGF-beta1 were present on 55 and 65% of human epithelial cell line cells, respectively, over a 1-log-unit range of receptor protein expression, as shown by flow cytometry and confirmed by confocal microscopy; (ii) only basolateral and not apical TGF-beta1 exposure of the polarized epithelial monolayer resulted in a protective effect; and (iii) TGF-beta1 had no direct effect on the organism in reducing its tissue-disruptive effects. In exploring mechanisms to account for the barrier-preserving effects of TGF-beta1 on epithelium, we found that the protein kinase C pathway was activated, as shown by translocation of its 80-kDa alpha isozyme within 30 s of epithelial exposure to TGF-beta1; the permeability of epithelial monolayers to passage of macromolecules was reduced by 42% with TGF-beta1, even in the face of active protozoal infection; and epithelial cell necrosis monitored by lactate dehydrogenase release was decreased by 50% 70 h after oocyst exposure. Changes in epithelial function, initiated through an established set of surface receptors, likely accounts for the remarkable barrier-sparing effect of nanogram-per-milliliter concentrations of TGF-beta1 when human colonic epithelium is exposed to an important human pathogen, C. parvum.
authors.affiliation: Divisions of Gastroenterology and of Geographic and International Medicine, Department of Medicine, University of Virginia Health System, Charlottesville, Virginia 22908, USA. jkr7m@virginia.edu
authors.forename: J K
authors.initials: JK
authors.lastname: Roche
authors.forename: C A
authors.initials: CA
authors.lastname: Martins
authors.forename: R
authors.initials: R
authors.lastname: Cosme
authors.forename: R
authors.initials: R
authors.lastname: Fayer
authors.forename: R L
authors.initials: RL
authors.lastname: Guerrant
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000187:drug effects*; D016764:Cell Polarity; D003106:Colon / Q000276:immunology* / Q000469:parasitology; D016785:Cryptosporidium parvum / Q000254:growth & development / Q000472:pathogenicity*; D004789:Enzyme Activation; D004847:Epithelial Cells / Q000201:enzymology / Q000469:parasitology / Q000502:physiology; D006801:Humans; D007413:Intestinal Mucosa / Q000276:immunology* / Q000469:parasitology / Q000502:physiology; D009336:Necrosis; D011493:Protein Kinase C / Q000378:metabolism; D018125:Receptors, Transforming Growth Factor beta / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
chemical_list: D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta; D011493:Protein Kinase C