title: [Adaptation of insect cells(BTI-Tn-5B1-4) to serum-free culture].
abstract: The insect cells(BTI-Tn-5B1-4) were adapted, from the medium supplemented with 5% FBS, via 3%, 2%, 1% FBS, to grow in serum-free medium by sequential adaptation for about three months. After adaptation, the cells could successfully grow in the specifically designed serum-free medium with the viability above 90%. In addition, the effect of FBS concentrations and inoculum sizes on the insect cell growth was also investigated.
authors.affiliation: State Key Lab of Bioreactor Engineering, East China University of Science & Technology, Shanghai.
authors.forename: H
authors.initials: H
authors.lastname: Dai
authors.forename: J
authors.initials: J
authors.lastname: Zhao
authors.forename: W S
authors.initials: WS
authors.lastname: Tan
authors.forename: Y Z
authors.initials: YZ
authors.lastname: Yang
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D007313:Insecta / Q000166:cytology*
chemical_list: D016895:Culture Media, Serum-Free