A novel human multiple myeloma-derived cell line, NCU-MM-1, carrying t(2;11)(q11;q23) and t(8;22)(q24;q11) chromosomal translocations with overexpression of c-Myc protein.
A novel cell line, designated as NCU-MM-1, was established from a 66-year-old female patient with multiple myeloma (MM) that had shown rapid progression from solitary plasmacytoma to plasma cell leukemia. Interestingly, cytogenetic analysis including fluorescence in situ hybridization analysis disclosed that this cell line carried 2 kinds of chromosomal translocations involving immunoglobulin light chain (IgL) gene loci without the presence of 14q32 translocations (14q+). The Ig lambda locus juxtaposed to the c-MYC locus at 8q24 on the derivative (8) chromosome and a concomitant overexpression of the c-Myc protein was observed. On the derivative (11) chromosome, the Ig kappa locus was also fused to the chromosome 11q23 locus, which is known to be a nonrandom translocation breakpoint in mature B-cell malignancies. The NCU-MM-1 cell line may thus be useful not only for the identification of the responsible proto-oncogene(s) mapped to 11q23, deregulated by the Ig kappa enhancer sequences, but also for clarification of the molecular origin of MM lacking 14q+ chromosomes because IgL rearrangements can physiologically begin to occur in the pre-B-cell stage.
Iida
S
S
Second Department of Internal Medicine, Nagoya City University Medical School, Japan. iida@med.nagoya-cu.ac.jp
Hanamura
I
I
Suzuki
T
T
Kamiya
T
T
Kato
M
M
Hayami
Y
Y
Miura
K
K
Harada
S
S
Tsuboi
K
K
Wakita
A
A
Akano
Y
Y
Taniwaki
M
M
Nitta
M
M
Ueda
R
R
D000368:Aged; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D002880:Chromosomes, Human, Pair 11; D002889:Chromosomes, Human, Pair 2; D002898:Chromosomes, Human, Pair 8; D020732:Cytogenetic Analysis; D005260:Female; D015870:Gene Expression; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000235:genetics; D009101:Multiple Myeloma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D000090063:Proto-Oncogene Mas; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics* / Q000378:metabolism; D014178:Translocation, Genetic / Q000235:genetics*
D007147:Immunoglobulin Light Chains; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D016271:Proto-Oncogene Proteins c-myc