Effect of collection, transport, processing and storage of blood specimens on the activity of lysosomal enzymes in plasma and leukocytes.
This study was designed to evaluate the effect of different conditions of collection, transport and storage on the quality of blood samples from normal individuals in terms of the activity of the enzymes ss-glucuronidase, total hexosaminidase, hexosaminidase A, arylsulfatase A and ss-galactosidase. The enzyme activities were not affected by the different materials used for collection (plastic syringes or vacuum glass tubes). In the evaluation of different heparin concentrations (10% heparin, 5% heparin, and heparinized syringe) in the syringes, it was observed that higher doses resulted in an increase of at least 1-fold in the activities of ss-galactosidase, total hexosaminidase and hexosaminidase A in leukocytes, and ss-glucuronidase in plasma. When the effects of time and means of transportation were studied, samples that had been kept at room temperature showed higher deterioration with time (72 and 96 h) before processing, and in this case it was impossible to isolate leukocytes from most samples. Comparison of heparin and acid citrate-dextrose (ACD) as anticoagulants revealed that ss-glucuronidase and hexosaminidase activities in plasma reached levels near the lower normal limits when ACD was used. In conclusion, we observed that heparin should be used as the preferable anticoagulant when measuring these lysosomal enzyme activities, and we recommend that, when transport time is more than 24 h, samples should be shipped by air in a styrofoam box containing wet ice.
Burin
M
M
Serviço de Genética Médica, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil. mburin@hcpa.ufrgs.br
Dutra-Filho
C
C
Brum
J
J
Mauricio
T
T
Amorim
M
M
Giugliani
R
R
D000293:Adolescent; D000328:Adult; D000925:Anticoagulants; D001800:Blood Specimen Collection / Q000379:methods; D002553:Cerebroside-Sulfatase / Q000097:blood*; D019343:Citric Acid; D005260:Female; D006026:Glycoside Hydrolases / Q000097:blood*; D006493:Heparin; D054818:Hexosaminidase A; D006801:Humans; D007962:Leukocytes / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D001616:beta-Galactosidase / Q000097:blood; D001619:beta-N-Acetylhexosaminidases / Q000097:blood
D000925:Anticoagulants; D019343:Citric Acid; D006493:Heparin; D002553:Cerebroside-Sulfatase; D006026:Glycoside Hydrolases; D001616:beta-Galactosidase; D054818:Hexosaminidase A; D001619:beta-N-Acetylhexosaminidases