Expression of cyclooxygenase-1 and -2 mRNA in rat tissues: tissue-specific difference in the expression of the basal level of mRNA.
The expression of cyclooxygenase (Cox)-1 and -2 mRNA was studied in tissues of Fisher 344 (F344) (n=3) and Brown Norway (BN) (n=3) strain rats using the reverse transcription polymerase chain reaction. Cox-1 mRNA was expressed in the liver, kidney and lung, but not in the heart. Cox-2 mRNA was expressed in the kidney, heart and lung, but not in the liver. The present results showed the differential expression of basal Cox-1 and Cox-2 mRNA in the rat tissues, suggesting that Cox-1 and Cox-2 may contribute to the regulation of normal pathophysiological conditions in a tissue-specific manner. Thus, it is desirable to perform mapping of the basal expression of Cox-1 and Cox-2 in specific organs in the body in order to anticipate the effects of non-steroidal anti-inflammatory drugs on normal pathophysiological conditions. Finally, no difference was observed between F344 and BN rat tissues in the levels of Cox-1 and Cox-2 mRNA expression.
Okamoto
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Research Laboratories, Nippon Chemiphar Co., Ltd., Saitama 341-0005, Japan.
Hino
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D000818:Animals; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D015971:Gene Expression Regulation, Enzymologic; D007527:Isoenzymes / Q000235:genetics*; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D008565:Membrane Proteins; D009206:Myocardium / Q000201:enzymology; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D011914:Rats, Inbred BN; D011916:Rats, Inbred F344; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014018:Tissue Distribution
D007527:Isoenzymes; D008565:Membrane Proteins; D012333:RNA, Messenger; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases; C496513:Ptgs1 protein, rat