Identification of the human Y-chromosomal microsatellite locus DYS19 from degraded DNA.
STR DYS19 seems to be one of the most useful markers for population genetic, evolutionary, and forensic applications. However, the authors have noticed that the amplification of the DYS19 polymorphism fails when highly degraded DNA is used as a template. The authors designed a new pair of primers that reduce the DYS19 fragment sizes compared with those of the known protocol. Using these primers, an improved success rate can be achieved, particularly when putrefied samples are under investigation.
Szibor
R
R
Institut für Rechtsmedizin, Otto-von-Guericke-Universität, Magdeburg, Germany.
Kayser
M
M
Roewer
L
L
D000483:Alleles; D001344:Autopsy / Q000379:methods; D001483:Base Sequence; D016384:Consensus Sequence; D004247:DNA / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D005554:Forensic Medicine / Q000379:methods; D005819:Genetic Markers; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D013698:Templates, Genetic; D014998:Y Chromosome / Q000235:genetics*
D017931:DNA Primers; D005819:Genetic Markers; D004247:DNA