Comparison of blood-brain barrier permeability in mice and rats using in situ brain perfusion technique.
Here we present a method for measuring the permeability coefficient-surface area product (PS) values at the blood-brain barrier in mice, using the in situ brain perfusion technique originally developed for rats by Takasato et al. (Am J Physiol Heart Circ Physiol 247: H484-H493, 1984). Retrograde infusion into the right external carotid artery increased the carotid perfusion pressure in proportion to the perfusion rate. Intravascular volume and cerebral perfusion fluid flow at a perfusion rate of 1.0 ml/min in mice were similar to those in rats. In addition, the contribution of systemic blood to total flow in the hemisphere was small (only 3. 2%). These findings indicated that this perfusion rate is suitable for mice. The PS values of more than 20 different compounds were determined in mice by using the in situ brain perfusion technique, and comparisons were made with data from rats. There was a close relationship (1:1) between the PS values in mice and rats, indicating that brain capillary permeabilities are similar in mice and rats.
Murakami
H
H
Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Takanaga
H
H
Matsuo
H
H
Ohtani
H
H
Sawada
Y
Y
D000818:Animals; D000983:Antipyrine / Q000008:administration & dosage / Q000097:blood; D001692:Biological Transport / Q000502:physiology; D001783:Blood Flow Velocity / Q000502:physiology; D001794:Blood Pressure / Q000502:physiology; D001812:Blood-Brain Barrier / Q000187:drug effects / Q000502:physiology*; D001921:Brain / Q000098:blood supply* / Q000378:metabolism / Q000502:physiology; D001923:Brain Chemistry; D002199:Capillary Permeability / Q000187:drug effects / Q000502:physiology*; D002339:Carotid Arteries / Q000502:physiology; D002560:Cerebrovascular Circulation / Q000502:physiology; D007275:Injections, Intravenous; D007444:Inulin / Q000008:administration & dosage / Q000097:blood; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D000442:Octanols / Q000737:chemistry; D010477:Perfusion / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism; D010599:Pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D013045:Species Specificity; D014867:Water / Q000737:chemistry
D000442:Octanols; D004364:Pharmaceutical Preparations; D014867:Water; D007444:Inulin; D000983:Antipyrine