[New cyclomaltodextrin-glucanotransferases, produced by Bacillus macerans].
For the first time, microorganisms producing cyclomaltodextrin glucan transferases (CGT, EC 2.4.1.19) were isolated from soil samples of various ecogeographical regions. These microorganisms were identified as Bacillus macerans. The enzymes were purified by affinity chromatography on a alpha-cyclodextrin polymer and gel filtration on Biogel P-450 and proved to be electrophoretically homogeneous. Some of their physicochemical and biochemical properties are reported.
Abelian
V A
VA
Institute of Microbiology, National Academy of Sciences of Armenia, Abovyan, Armenia.
Afian
K B
KB
Manukian
L S
LS
D000975:Antioxidants / Q000302:isolation & purification / Q000378:metabolism; D001407:Bacillus / Q000378:metabolism*; D002846:Chromatography, Affinity / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005964:Glucosyltransferases / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D013696:Temperature
D000975:Antioxidants; D004791:Enzyme Inhibitors; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase