Chronic metabolic sequelae of traumatic brain injury: prolonged suppression of somatosensory activation.
Injuries to the brain acutely disrupt normal metabolic function and may deactivate functional circuits. It is unknown whether these metabolic abnormalities improve over time. We used 2-deoxyglucose (2-DG) autoradiographic image-averaging to assess local cerebral glucose utilization (lCMR(Glc)) of the rat brain 2 mo after moderate (1.7-2.1 atm) fluid-percussion traumatic brain injury (FPI). Four animal groups (n = 5 each) were studied: sham-injured rats with and without stimulation of the vibrissae-barrel field ipsilateral to injury; and animals with prior FPI, with or without this stimulation. In sham-injured rats, resting lCMR(Glc) was normal, and vibrissae stimulation produced right-sided metabolic activation of the ventrolateral thalamic and somatosensory-cortical projection areas. In rats with prior injury, lCMR(Glc) contralateral to injury was normal, but lCMR(Glc) of the ipsilateral forebrain was depressed by approximately 38-45% compared with shams. Whisker stimulation in rats with prior trauma failed to induce metabolic activation of either cortex or thalamus. Image-mapping of histological material obtained in the same injury model was undertaken to assess the possible influence of injury-induced regional brain atrophy on computed lCMR(Glc); an effect was found only in the lateral cortex at the trauma epicenter. Our results show that, 2 mo after trauma, resting cerebral metabolic perturbations persist, and the whisker-barrel somatosensory circuit shows no signs of functional recovery.
Passineau
M J
MJ
Neurotrauma Research Center, University of Miami School of Medicine, Miami, Florida 33101, USA.
Zhao
W
W
Busto
R
R
Dietrich
W D
WD
Alonso
O
O
Loor
J Y
JY
Bramlett
H M
HM
Ginsberg
M D
MD
D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000378:metabolism; D001930:Brain Injuries / Q000378:metabolism*; D002908:Chronic Disease; D003847:Deoxyglucose / Q000493:pharmacokinetics; D004195:Disease Models, Animal; D005947:Glucose / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D008297:Male; D010296:Parietal Lobe / Q000293:injuries; D010812:Physical Stimulation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013003:Somatosensory Cortex / Q000378:metabolism*
D003847:Deoxyglucose; D005947:Glucose