Virus inactivation by anilinonaphthalene sulfonate compounds and comparison with other ligands.
Bis-(8-anilinonaphthalene-1-sulfonate) (bis-ANS) causes inactivation of vesicular stomatitis virus (VSV) at micromolar concentrations while butyl-ANS and ANS are effective at concentrations one and two orders of magnitude higher, respectively. VSV fully inactivated by the combined effects of 10 microM bis-ANS and 2.5 kbar hydrostatic pressure elicited a high titer of neutralizing antibodies. Incubation of VSV with >/=2 M urea at atmospheric pressure caused very little virus inactivation, whereas at a pressure of 2.5 kbar, 1 M urea caused inactivation that exceeded by more than two orders of magnitude the sum of the inactivating effects produced by urea and pressure separately. Measurements of bis-ANS fluorescence showed that increasing the urea concentration reduces the pressure required to disrupt the structure. We conclude that anilinonaphthalene sulfonate compounds inactivate VSV by a mechanism similar to that produced by pressure. The most effective antiviral compound was bis-ANS which can be used for the preparation of safe viral vaccines or as an antiviral drug eventually.
Bonafe
C F
CF
Department of Biochemistry, University of Illinois, Urbana, Illinois 61801, USA. bonafe@unicamp.br
Glaser
M
M
Voss
E W
EW
Weber
G
G
Silva
J L
JL
D000817:Anilino Naphthalenesulfonates / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D000998:Antiviral Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use; D002460:Cell Line; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D005453:Fluorescence; D006874:Hydrostatic Pressure; D012709:Serum Albumin / Q000494:pharmacology; D014508:Urea / Q000494:pharmacology; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects* / Q000276:immunology / Q000502:physiology; D014994:Xylose / Q000494:pharmacology
D000817:Anilino Naphthalenesulfonates; D000906:Antibodies; D000998:Antiviral Agents; D012709:Serum Albumin; C027132:1-anilino-8-naphthalenesulfonate; C035763:5,5'-bis(8-(phenylamino)-1-naphthalenesulfonate); D014508:Urea; D014994:Xylose