PCR-based subtractive hybridization identifies repressed genes in growth-arrested human dermal fibroblasts following combined treatment with 8-methoxypsoralen and UVA irradiation (PUVA).
To identify genes which are repressed in growth-arrested human dermal fibroblasts upon a single treatment with 8-methoxypsoralen and UVA irradiation (PUVA) we have used a PCR-based subtractive hybridization protocol resulting in cloning of four PUVA-repressed genes. Sequence analysis and homology searches identified three known genes related to growth control, lipid and connective tissue metabolism. One cDNA clone represented a novel gene. Northern blot analyses confirmed a PUVA-dependent reduction in mRNA expression in fibroblasts in vitro. The identification of growth arrest related repressed genes in PUVA-treated fibroblasts may stimulate further research addressing the causal role of these genes in the control and regulation of the postmitotic phenotype of fibroblasts on a molecular and cellular level.
Wlaschek
M
M
Department of Dermatology, University of Cologne, Joseph-Stelzmann-Strasse 9, 50931, Cologne, Germany.
Wenk
J
J
Brenneisen
P
P
Hommel
C
C
Ma
W
W
Herrmann
G
G
Scharffetter-Kochanek
K
K
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects*; D002648:Child; D002675:Child, Preschool; D018076:DNA, Complementary / Q000032:analysis; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D007797:Laminin / Q000502:physiology; D008297:Male; D009693:Nucleic Acid Hybridization; D011701:PUVA Therapy; D016133:Polymerase Chain Reaction; D012867:Skin / Q000187:drug effects*
D018076:DNA, Complementary; D007797:Laminin