A mouse bone marrow stromal cell line, TBR-B, shows inducible expression of smooth muscle-specific genes.
We established an in vitro culture system which mimicked the differentiation pathway of smooth muscle cell, using TBR-B, a bone marrow stromal cell line derived from transgenic mice harboring temperature-sensitive SV40 large T-antigen gene. TBR-B cells have the potential to express smooth muscle-specific genes including h1-calponin, h-caldesmon, SM22alpha and alpha-actin, only after cultured in the differentiation medium for 2 weeks. The differentiation state of TBR-B was well controlled by using different culture medium. Using this cell line, we also found that ascorbic acid is a potent factor inducing the expression of h1-calponin and alpha-actin. TBR-B cells will serve as a useful tool for elucidating the regulatory mechanisms of smooth muscle-specific gene expression, and for identifying compounds that regulate the differentiation state of vascular smooth muscle cells.
Arakawa
E
E
Vessel Research Laboratory Co., Ltd. Asahimachi, Machida, Tokyo 194-8533, Japan. emi.arakawa@kyowa.co.jp
Hasegawa
K
K
Yanai
N
N
Obinata
M
M
Matsuda
Y
Y
D000199:Actins / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D015415:Biomarkers / Q000032:analysis; D015153:Blotting, Western; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism; D002148:Calmodulin-Binding Proteins / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D003470:Culture Media / Q000494:pharmacology; D007150:Immunohistochemistry; D051379:Mice; D008822:Mice, Transgenic; D008840:Microfilament Proteins / Q000235:genetics; D009124:Muscle Proteins / Q000235:genetics; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D009928:Organ Specificity; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects; D000096985:Calponins
D000199:Actins; D001205:Ascorbic Acid; D015415:Biomarkers; D002135:Calcium-Binding Proteins; D002148:Calmodulin-Binding Proteins; D003470:Culture Media; D008840:Microfilament Proteins; D009124:Muscle Proteins; D012333:RNA, Messenger; D000096985:Calponins; C058992:Tagln protein, mouse; C051615:transgelin