Cross-reactivity between Coxiella burnetii and chlamydiae.
A cross-reactivity among some strains of Coxiella burnetii and chlamydiae with immune rabbit and mouse sera in ELISA and immunoblot analysis was observed. In the latter, the cross-reactivity disappeared after a treatment of C. burnetii or C. psittaci with proteinase K, which indicates that only proteins were involved. The observed cross-reactivity was not influenced by host chick embryo yolk sac proteins. After adsorption of immune rabbit sera with homologous corpuscular antigens the cross-reactivity disappeared. The possibility of influence of such cross-reactivity on serological diagnosis of C. burnetii or chlamydiae infections is discussed.
Lukácová
M
M
Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovakia. viruluka@savba.savba.sk
Melnicáková
J
J
Kazár
J
J
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D002689:Chlamydia / Q000276:immunology*; D002690:Chlamydia Infections / Q000382:microbiology; D016997:Coxiella burnetii / Q000276:immunology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007106:Immune Sera / Q000276:immunology*; D015151:Immunoblotting; D051379:Mice; D008807:Mice, Inbred BALB C; D011778:Q Fever / Q000382:microbiology; D011817:Rabbits
D000942:Antigens, Bacterial; D007106:Immune Sera