title: Short bowel syndrome: experimental approach to increase intestinal surface in rats by gastric homologous acellular matrix.
abstract: OBJECTIVE
  In this preliminary work the authors used homologous acellular matrix obtained by the gastric wall to increase the small bowel surface in Sprague-Downey rats; through this experimental model the authors verified that homologous acellular matrix can support cell migration and the reconstruction of the intestinal wall.
  
  
  METHODS
  A tract of about 2 cm of tubular gastric acellular matrix was inserted with bilateral anastomosis in an isolated ileal loop, which was located in endoabdominal position through a short subcutaneous tunnel. Twelve animals were analyzed at each of the time-points ranging from 1 to 6 weeks after surgery.
  
  
  RESULTS
  Histologic evaluation showed that the implanted matrix can be reintegrated in the normal small bowel in a period ranging between 3 and 6 weeks from surgery. The implanted matrix was organized with 4 different tonacae from the third week after the surgery, without interruption at the site of the anastomosis.
  
  
  CONCLUSIONS
  To date, the authors do not have a demonstration of the function of the ileal loop reconstructed with this technique; based on these results the authors are engaged in an experimental trial of restoration of intestinal viability with the ileal prosthesis after 3 weeks to study its function.
authors.affiliation: Department of Pharmaceutical Sciences, University of Padua, Italy.
authors.forename: P P
authors.initials: PP
authors.lastname: Parnigotto
authors.forename: M
authors.initials: M
authors.lastname: Marzaro
authors.forename: T
authors.initials: T
authors.lastname: Artusi
authors.forename: G
authors.initials: G
authors.lastname: Perrino
authors.forename: M T
authors.initials: MT
authors.lastname: Conconi
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use*; D007081:Ileostomy / Q000379:methods*; D007082:Ileum / Q000502:physiology; D008297:Male; D019919:Prosthesis Implantation / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration; D012778:Short Bowel Syndrome / Q000601:surgery*; D014184:Transplantation, Homologous
chemical_list: D001672:Biocompatible Materials