An increase in pectin methyl esterase activity accompanies dormancy breakage and germination of yellow cedar seeds.
Pectin methyl esterase (PME) (EC 3.1.1.11) catalyzes the hydrolysis of methylester groups of cell wall pectins. We investigated the role of this enzyme in dormancy termination and germination of yellow cedar (Chamaecyparis nootkatensis [D. Don] Spach) seeds. PME activity was not detected in dormant seeds of yellow cedar but was induced and gradually increased during moist chilling; high activity coincided with dormancy breakage and germination. PME activity was positively correlated to the degree of dormancy breakage of yellow cedar seeds. The enzyme produced in different seed parts and in seeds at different times during moist chilling, germination, and early post-germinative growth consisted of two isoforms, both basic with isoelectric points of 8.7 and 8.9 and the same molecular mass of 62 kD. The pH optimum for the enzyme was between 7.4 and 8.4. In intact yellow cedar seeds, activities of the two basic isoforms of PME that were induced in embryos and in megagametophytes following dormancy breakage were significantly suppressed by abscisic acid. Gibberellic acid had a stimulatory effect on the activities of these isoforms in embryos and megagametophytes of intact seeds at the germinative stage. We hypothesize that PME plays a role in weakening of the megagametophyte, allowing radicle emergence and the completion of germination.
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Department of Biological Sciences, Simon Fraser University, 8888 University Drive, Burnaby, British Columbia, Canada V5A 1S6.
Kermode
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D000040:Abscisic Acid / Q000378:metabolism; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D003080:Cold Temperature; D004586:Electrophoresis / Q000379:methods; D004795:Enzyme Stability; D018525:Germination; D005875:Gibberellins / Q000378:metabolism; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D012639:Seeds / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D014197:Trees / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*
D005875:Gibberellins; D007527:Isoenzymes; D010940:Plant Proteins; D000040:Abscisic Acid; C007842:gibberellic acid; D002265:Carboxylic Ester Hydrolases; C022323:pectinesterase