title: Synaptic targeting and localization of discs-large is a stepwise process controlled by different domains of the protein.
abstract: BACKGROUND
  Membrane-associated guanylate kinases (MAGUKs) assemble ion channels, cell-adhesion molecules and components of second messenger cascades into synapses, and are therefore potentially important for co-ordinating synaptic strength and structure. Here, we have examined the targeting of the Drosophila MAGUK Discs-large (DLG) to larval neuromuscular junctions.
  
  
  RESULTS
  During development, DLG was first found associated with the muscle subcortical compartment and plasma membrane, and later was recruited to the postsynaptic membrane. Using a transgenic approach, we studied how mutations in various domains of the DLGprotein affect DLG targeting. Deletion of the HOOK region-the region between the Src homology 3 (SH3) domain and the guanylate-kinase-like (GUK) domain-prevented association of DLG with the subcortical network and rendered the protein largely diffuse. Loss of the first two PDZ domains led to the formation of large clusters throughout the plasma membrane, with scant targeting to the neuromuscular junction. Proper trafficking of DLG missing the GUK domain depended on the presence of endogenous DLG.
  
  
  CONCLUSIONS
  Postsynaptic targeting of DLG requires a HOOK-dependent association with extrasynaptic compartments, and interactions mediated by the first two PDZ domains. The GUK domain routes DLG between compartments, possibly by interacting with recently identified cytoskeletal-binding partners.
authors.affiliation: Department of Biology, University of Massachusetts, Amherst, 01003, Massachusetts, USA.
authors.forename: U
authors.initials: U
authors.lastname: Thomas
authors.forename: S
authors.initials: S
authors.lastname: Ebitsch
authors.forename: M
authors.initials: M
authors.lastname: Gorczyca
authors.forename: Y H
authors.initials: YH
authors.lastname: Koh
authors.forename: C D
authors.initials: CD
authors.lastname: Hough
authors.forename: D
authors.initials: D
authors.lastname: Woods
authors.forename: E D
authors.initials: ED
authors.lastname: Gundelfinger
authors.forename: V
authors.initials: V
authors.lastname: Budnik
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002462:Cell Membrane / Q000378:metabolism; D004330:Drosophila / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins; D051528:Guanylate Kinases; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000276:immunology / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism; D009469:Neuromuscular Junction; D009703:Nucleoside-Phosphate Kinase / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000378:metabolism; D017384:Sequence Deletion / Q000235:genetics; D013569:Synapses / Q000378:metabolism*; D025521:Tumor Suppressor Proteins
chemical_list: D029721:Drosophila Proteins; D019476:Insect Proteins; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; D025521:Tumor Suppressor Proteins; C070350:dlg1 protein, Drosophila; D009703:Nucleoside-Phosphate Kinase; D051528:Guanylate Kinases