[Electroporation of sperm to introduce foreign DNA into the genome of Pinctada maxima (Jameson)].
Gene transfer was investigated in marine molluscs via electroporated sperm. Sperm of P. maxima (J.) was incubated with linear "all-fish" growth hormone gene (pCAgcGH and pCAgcGHc) for 30 min. Then, mature eggs were in-vitro fertilized with the sperm cells treated with electroporation at 10 kV and 2(7) pulses of six cycles. DNA was extracted from spat and analyzed by PCR and southern blot. The results indicated that the foreign DNA had been transferred into the genome of experimental molluscs. The transgenetic ration was 5.6%, 20% and 50% when 2 micrograms/mL, 6 micrograms/mL and 18 micrograms/mL of foreign DNA was used, respectively. It is suggested that the transferred efficiency is correlated with the amount of the foreign DNA.
Hu
W
W
Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan.
Yu
D H
DH
Wang
Y P
YP
Wu
K C
KC
Zhu
Z Y
ZY
D000818:Animals; D018274:Electroporation; D018014:Gene Transfer Techniques; D008297:Male; D008974:Mollusca / Q000235:genetics*; D013094:Spermatozoa / Q000378:metabolism*