Interleukin-6-induced tyrosine phosphorylation of phospholipase C-gamma1 in PC12 cells.
Phospholipase C (PLC)-gamma1 plays a pivotal role in the signal transduction pathway mediated by growth factors. In this study, we found that neurite outgrowth of pheochromocytoma (PC12) cells was significantly induced by interleukin-6 (IL-6). Stimulation of PC12 cells with IL-6 led to tyrosine phosphorylation of PLC-gamma1 in a dose- and time-dependent manner. IL-6 stimulation also increased the hydrolysis of phosphatidylinositol 4,5-bisphosphate. Accumulation of total inositol phosphate as well as tyrosine phosphorylation of PLC-gamma1 was inhibited by the pretreatment of protein kinase inhibitors such as genistein and staurosporine. These results suggest that PLC-gamma1 may be involved in the signal transduction pathway of IL-6-induced PC12 cell differentiation.
Lee
Y H
YH
Department of Life Science and School of Environmental Engineering, Pohang University of Science and Technology, Korea.
Bae
S S
SS
Seo
J K
JK
Choi
I
I
Ryu
S H
SH
Suh
P G
PG
D000818:Animals; D019833:Genistein / Q000494:pharmacology; D007295:Inositol Phosphates / Q000378:metabolism; D015850:Interleukin-6 / Q000494:pharmacology*; D007527:Isoenzymes / Q000378:metabolism / Q000502:physiology*; D016501:Neurites / Q000187:drug effects / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D016716:PC12 Cells; D051966:Phospholipase C gamma; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000187:drug effects / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D051381:Rats; D015398:Signal Transduction; D010738:Type C Phospholipases / Q000378:metabolism / Q000502:physiology*
D007295:Inositol Phosphates; D015850:Interleukin-6; D007527:Isoenzymes; D019000:Phosphotyrosine; D019833:Genistein; D011505:Protein-Tyrosine Kinases; D010738:Type C Phospholipases; D051966:Phospholipase C gamma