Purification of alkaline phosphatase from chicken intestine by expanded-bed affinity chromatography on dye-linked cellulose.
Alkaline phosphatase from chicken intestine was purified on dye-linked cellulose beads. The various dye-linked cellulose beads were screened in the batch mode for purification of the enzyme and the promising dyes were tried in the expanded-bed mode. The one-step expanded-bed affinity chromatography on Cibacron Blue-linked cellulose beads yielded 48-fold purification with an activity recovery of 70%.
Roy
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Chemistry Department, Indian Institute of Technology, Hauz Khas, New Delhi 110 016, India.
Gupta
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MN
D000327:Adsorption; D000469:Alkaline Phosphatase / Q000302:isolation & purification* / Q000378:metabolism; D000818:Animals; D002482:Cellulose / Q000737:chemistry; D002645:Chickens; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D004396:Coloring Agents / Q000737:chemistry / Q000378:metabolism; D007422:Intestines / Q000201:enzymology*; D014227:Triazines / Q000737:chemistry / Q000378:metabolism
D004396:Coloring Agents; D014227:Triazines; C012842:Cibacron Blue F 3GA; D002482:Cellulose; D000469:Alkaline Phosphatase