Depletion of brain glutathione potentiates the effect of 6-hydroxydopamine in a rat model of Parkinson's disease.
In order to examine the possible role of rat brain glutathione depletion by diethyl maleate (DEM) in the potentiation of 6-hydroxydopamine (6-OHDA) neurotoxicity, the relationships between both effects were evaluated using the circling behavior test (CBT), and determining striatal glutathione S-transferase (GST)-specific activity. There were significant differences between the two studied groups: 6-OHDA and DEM + 6-OHDA lesioned animals in striatal glutathione (GSH) concentration at the moment of the lesion with 6-OHDA and also at the end of the experiment (30 d after 6-OHDA lesion). The circling behavior test following the administration of amphetamine was qualitatively different between both groups of simple- and double-damaged animals. In accordance with our results, DEM injury makes the animals more susceptible to brain-oxidative damage by 6-OHDA, which can indicate that in the double-damaged animal group, DEM could induce potentiation of the toxicity through striatal glutathione depletion.
García
J C
JC
Neurotoxicologia, CETEX-CENPALAB, Havana, Cuba. neurotox@cenpalab.inf.cu
Remires
D
D
Leiva
A
A
González
R
R
D000818:Animals; D001522:Behavior, Animal / Q000502:physiology; D001923:Brain Chemistry / Q000187:drug effects; D003342:Corpus Striatum / Q000201:enzymology*; D005978:Glutathione / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D008298:Maleates / Q000494:pharmacology; D016627:Oxidopamine; D020734:Parkinsonian Disorders / Q000139:chemically induced / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013378:Substantia Nigra / Q000201:enzymology; D013565:Sympatholytics
D008298:Maleates; D013565:Sympatholytics; D016627:Oxidopamine; D005982:Glutathione Transferase; C014476:diethyl maleate; D005978:Glutathione