The development of a cell array and its combination with laser-scanning cytometry allows a high-throughput analysis of nuclear DNA content.
We developed a cell array device for analyzing cellular characteristics such as DNA ploidy, numerical chromosomal aberrations, and antigen expression in multiple specimens in a single experiment. Fifty (10 x 5) spots, 2 mm in diameter, were arrayed in an area of 30 x 16 mm on a glass slide, and approximately 1,000 cells were placed on each spot. To demonstrate the usefulness of the cell array, we measured nuclear DNA content using laser-scanning cytometry for DNA ploidy analysis in nine human tumor cell lines and normal lymphocytes. Combining the cell array with laser-scanning cytometry allows not only measurement of nuclear DNA content for 50 samples but also easy comparison of DNA ploidy among the samples in a single experiment. In addition, we used the cell array for fluorescence in situ hybridization using a DNA probe specific for the pericentromeric region of chromosome 11.
Oode
K
K
Departments of Pathology and Surgery, Yamaguchi University School of Medicine, Ube. Tsukuba, Ibaraki, Japan.
Furuya
T
T
Harada
K
K
Kawauchi
S
S
Yamamoto
K
K
Hirano
T
T
Sasaki
K
K
D002452:Cell Count; D002467:Cell Nucleus / Q000235:genetics*; D002503:Centromere / Q000235:genetics; D002880:Chromosomes, Human, Pair 11; D004273:DNA, Neoplasm / Q000235:genetics*; D006801:Humans; D019044:Image Cytometry / Q000295:instrumentation* / Q000379:methods; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes / Q000166:cytology; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods; D011003:Ploidies; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm