title: Cloning of the murine thymic stromal lymphopoietin (TSLP) receptor: Formation of a functional heteromeric complex requires interleukin 7 receptor.
abstract: The cellular receptor for murine thymic stromal lymphopoietin (TSLP) was detected in a variety of murine, but not human myelomonocytic cell lines by radioligand binding. cDNA clones encoding the receptor were isolated from a murine T helper cell cDNA library. TSLP receptor (TSLPR) is a member of the hematopoietin receptor family. Transfection of TSLPR cDNA resulted in only low affinity binding. Cotransfection of the interleukin 7 (IL-7)Ralpha chain cDNA resulted in conversion to high affinity binding. TSLP did not activate cells from IL-7Ralpha(-/)- mice, but did activate cells from gammac(-/)- mice. Thus, the functional TSLPR requires the IL-7Ralpha chain, but not the gammac chain for signaling.
authors.affiliation: Department of Biochemistry, Immunex Corporation, Seattle, Washington 98101, USA.
authors.forename: L S
authors.initials: LS
authors.lastname: Park
authors.forename: U
authors.initials: U
authors.lastname: Martin
authors.forename: K
authors.initials: K
authors.lastname: Garka
authors.forename: B
authors.initials: B
authors.lastname: Gliniak
authors.forename: J P
authors.initials: JP
authors.lastname: Di Santo
authors.forename: W
authors.initials: W
authors.lastname: Muller
authors.forename: D A
authors.initials: DA
authors.lastname: Largaespada
authors.forename: N G
authors.initials: NG
authors.lastname: Copeland
authors.forename: N A
authors.initials: NA
authors.lastname: Jenkins
authors.forename: A G
authors.initials: AG
authors.lastname: Farr
authors.forename: S F
authors.initials: SF
authors.lastname: Ziegler
authors.forename: P J
authors.initials: PJ
authors.lastname: Morrissey
authors.forename: R
authors.initials: R
authors.lastname: Paxton
authors.forename: J E
authors.initials: JE
authors.lastname: Sims
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D016207:Cytokines / Q000494:pharmacology; D006410:Hematopoiesis / Q000187:drug effects*; D006801:Humans; D015851:Interleukin-7 / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects* / Q000502:physiology; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D018121:Receptors, Cytokine / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D020395:Receptors, Interleukin-7 / Q000737:chemistry / Q000502:physiology*; D011994:Recombinant Proteins / Q000737:chemistry; D015398:Signal Transduction
chemical_list: D016207:Cytokines; D015851:Interleukin-7; D012333:RNA, Messenger; D018121:Receptors, Cytokine; D020395:Receptors, Interleukin-7; D011994:Recombinant Proteins