title: Enhanced chemiluminescent determination of chloramphenicol and related nitro compounds by 'on-line' photochemical reaction.
abstract: The viability of tandem photochemical reaction-chemiluminescence detection was studied for a heterogeneous family of nitro compounds using chloramphenicol as a test substance. The 'on-line' chemical photodegradation of chloramphenicol was performed in a flow injection assembly by using a photoreactor consisting of a 725 cm x 0.5 mm id piece of PTFE tubing coiled around an 8 W low-pressure mercury lamp. Photodegraded chloramphenicol was detected by oxidizing photo-fragments from the parent compound and their subsequent reaction with a luminol-Co(II) system. The calibration graph was linear up to 3 x 10(-5) mol l-1 chloramphenicol, the limit of detection was 3 x 10(-9) mol l-1, the relative standard deviation was 1.8% for 2 x 10(-6) mol l-1 of the drug and the sample throughput was 60 h-1. The proposed method was used to determine chloramphenicol in pharmaceutical formulations and its application to other related nitro compounds was studied.
authors.affiliation: Department of Analytical Chemistry, University of Bucharest, Romania.
authors.forename: V
authors.initials: V
authors.lastname: David
authors.forename: R M
authors.initials: RM
authors.lastname: Saèz
authors.forename: J V
authors.initials: JV
authors.lastname: Mateo
authors.forename: J M
authors.initials: JM
authors.lastname: Calatayud
mesh_terms: D002138:Calibration; D002701:Chloramphenicol / Q000032:analysis*; D008163:Luminescent Measurements; D009574:Nitro Compounds / Q000032:analysis; D010777:Photochemistry; D012815:Signal Processing, Computer-Assisted
chemical_list: D009574:Nitro Compounds; D002701:Chloramphenicol