title: Is double C2 protein (DOC2) expressed in bovine adrenal medulla? A commercial anti-DOC2 monoclonal antibody recognizes a major bovine mitochondrial antigen.
abstract: We have examined the expression in bovine adrenal medulla of double C2 protein (DOC2), a vesicular protein which associates with intracellular phospholipid and Ca(2+) and is implicated in the modulation of regulated exocytosis. Extensive reverse transcription-PCR, Northern blot analyses and in vitro translation reactions have been combined with immunological studies to provide data to suggest that neither DOC2alpha nor DOC2beta is expressed at detectable levels in bovine adrenal chromaffin cells, and that a widely used monoclonal antibody directed against DOC2 also recognizes mitochondrial complex III core protein 2.
authors.affiliation: Membrane Biology Group, University of Edinburgh, Medical School, Department of Biomedical Sciences, Edinburgh EH8 9AG, Scotland, UK. rory.duncan@ed.ac.uk
authors.forename: R R
authors.initials: RR
authors.lastname: Duncan
authors.forename: D K
authors.initials: DK
authors.lastname: Apps
authors.forename: M P
authors.initials: MP
authors.lastname: Learmonth
authors.forename: M J
authors.initials: MJ
authors.lastname: Shipston
authors.forename: R H
authors.initials: RH
authors.lastname: Chow
mesh_terms: D000313:Adrenal Medulla / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000941:Antigens / Q000276:immunology* / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D002417:Cattle; D019439:Chromaffin Cells / Q000276:immunology / Q000378:metabolism; D003429:Cross Reactions / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology; D051379:Mice; D008928:Mitochondria / Q000276:immunology* / Q000378:metabolism; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D002135:Calcium-Binding Proteins; C490418:Doc2a protein, mouse; D007106:Immune Sera; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger