Chaperone activity of tobacco HSP18, a small heat-shock protein, is inhibited by ATP.
NtHSP18P (HSP18), a cytosolic class I small heat-shock protein from tobacco pollen grains, was expressed in Escherichia coli. The viability of these cells was improved by 50% at 50 degrees C, demonstrating its functionality in vivo. Purified recombinant protein formed 240 kDa HSP18 oligomers, irrespective of temperature. These oligomers interacted with the model substrate citrate synthase (CS) to form large complexes in a temperature-dependent manner. Furthermore, HSP18 prevented thermally induced aggregation of CS at 45 degrees C. The fluorescence probe bis-ANS revealed the exposure of HSP18 hydrophobic surfaces at this temperature. Reactivation of chemically denatured CS was also significantly enhanced by HSP18. Surprisingly, HSP18 function was inhibited (in contrast to the related chaperone alphabeta-crystallin and plant sHSPs studied so far) by the presence of ATP in a concentration-dependent manner. The conformational changes of HSP18 imposed by ATP binding were indicated by the difference in the quenching of intrinsic tryptophan fluorescence, and implied more compact structure with ATP. Fluorescence measurements with bis-ANS showed that the conformational shift of HSP18 is suppressed in the presence of ATP. Decreased chaperone activity of HSP18 in the presence of ATP is caused by the lower affinity of conformationally blocked HSP18 for the substrate, as demonstrated by a higher susceptibility of model substrate, malate dehydrogenase, to proteolytic cleavage. Our results suggest that the chaperone activity of some plant sHSPs could be regulated by the availability of ATP in the cytoplasm, which would provide a mechanism to monitor the cell environment, control biological activity of sHSPs, and coordinate it with other ATP-dependent chaperones such as HSP70.
Smýkal
P
P
Department of Plant Physiology, Faculty of Science, Charles University, Vinièná 5, 128 44 Prague, Czech Republic.
Masín
J
J
Hrdý
I
I
Konopásek
I
I
Zárský
V
V
D000255:Adenosine Triphosphate / Q000502:physiology*; D001426:Bacterial Proteins; D003459:Crystallins / Q000502:physiology; D004926:Escherichia coli / Q000235:genetics; D006360:Heat-Shock Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D006868:Hydrolysis; D008291:Malate Dehydrogenase / Q000378:metabolism; D018832:Molecular Chaperones / Q000378:metabolism*; D010940:Plant Proteins; D010947:Plants, Toxic; D013696:Temperature; D014026:Nicotiana / Q000235:genetics*; D014364:Tryptophan / Q000502:physiology
D001426:Bacterial Proteins; D003459:Crystallins; C489905:HSP18 protein, plant; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D010940:Plant Proteins; C081475:hsp18 protein, bacteria; D014364:Tryptophan; D000255:Adenosine Triphosphate; D008291:Malate Dehydrogenase