Amadori-glycated albumin in diabetic nephropathy: pathophysiologic connections.
Amadori-glycated albumin in diabetic nephropathy: Pathophysiologic connections. Nonenzymatic glycation of proteins represents a major mechanism by which hyperglycemia leads to diabetic renal disease. Recent research has shown that Amadori-modified albumin, the principal glycated protein in plasma, elicits pathobiologic effects in cultured renal cells that are identical to those of high ambient glucose. When added to the incubation media of glomerular mesangial and endothelial cells, glycated albumin stimulates protein kinase C (PKC) activity, increases transforming growth factor-beta (TGF-beta) bioactivity, and induces gene overexpression and enhanced production of extracellular matrix proteins. These cellular events, whereby PKC-mediated TGF-beta activation leads to increased matrix expression, are inextricably linked, and they form the central tenets of a pathophysiologic connection between glycated proteins and diabetic nephropathy. In vivo studies further corroborate the role of glycated proteins in the pathogenesis of diabetic nephropathy. Reduction or neutralization of glycated albumin in the db/db mouse model of type 2 diabetes significantly ameliorates the proteinuria, renal insufficiency, mesangial expansion, and overexpression of matrix proteins. In human type 1 diabetes, the plasma-glycated albumin concentration is independently associated with the presence of nephropathy. Abrogating the biologic effects of increased glycated albumin has novel therapeutic potential in the management of renal complications in diabetes.
Chen
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S
Renal-Electrolyte and Hypertension Division and Penn Center for Molecular Stutdies of Kidney Diseases, University of Pennsylvania, and Institute of Metabolic Research and Exocell, Philadelphia, USA.
Cohen
M P
MP
Ziyadeh
F N
FN
D000818:Animals; D001483:Base Sequence; D003928:Diabetic Nephropathies / Q000209:etiology*; D004195:Disease Models, Animal; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis; D006031:Glycosylation; D006801:Humans; D007668:Kidney / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D011493:Protein Kinase C / Q000502:physiology; D011956:Receptors, Cell Surface / Q000378:metabolism; D012709:Serum Albumin / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000502:physiology
D016326:Extracellular Matrix Proteins; D011956:Receptors, Cell Surface; D012709:Serum Albumin; D016212:Transforming Growth Factor beta; D011493:Protein Kinase C