title: Identification and characterization of a mammalian protein interacting with 20S proteasome precursors.
abstract: The assembly of individual mammalian proteasome subunits into catalytically active 20S proteasome is not well understood. Herein, we report the identification and characterization of human and mouse homologues of the yeast proteasome maturating factor Ump1p. We delineate the region of hUMP1 implicated in the specific interaction with proteasome precursors and show that hUMP1 protein is absent from the mature form of the 20S proteasome. We also show that the transcript level of mammalian UMP1 is increased after IFN-gamma treatment and that mammalian UMP1 is functionally related to but not interchangeable with its yeast homologue.
authors.affiliation: Ludwig Institute for Cancer Research, Lausanne Branch, University of Lausanne, CH-1066 Epalinges, Switzerland.
authors.forename: L
authors.initials: L
authors.lastname: Burri
authors.forename: J
authors.initials: J
authors.lastname: Höckendorff
authors.forename: U
authors.initials: U
authors.lastname: Boehm
authors.forename: T
authors.initials: T
authors.lastname: Klamp
authors.forename: R J
authors.initials: RJ
authors.lastname: Dohmen
authors.forename: F
authors.initials: F
authors.lastname: Lévy
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D003001:Cloning, Molecular; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D018832:Molecular Chaperones / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017386:Sequence Homology, Amino Acid
chemical_list: D018832:Molecular Chaperones; D009097:Multienzyme Complexes; D012333:RNA, Messenger; C110856:proteasome maturation protein; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex