The chitinase gene of the silkworm, Bombyx mori, contains a novel Tc-like transposable element.
We have determined the cDNA sequence and the genomic organization of the chitinase gene of the silkworm, Bombyx mori. The cDNA encodes 544 amino acids having 83% amino acid homology to the chitinase of the tobacoo hornworm, Manduca sexta. The total length of the gene is larger than 25 kilobase pairs, and it is separated into 11 exons. The intron-exon boundaries are all in accordance with the GT-AG rule. Also, the TATA box sequence was found in the 5' upstream region of the gene, and the gene is mapped on the seventh chromosome. A novel DNA type transposon that shows similarity to the Tc-like element was found in the third intron in some strains of B. mori; other strains, however, lack this element in the same intron. This element has long terminal inverted repeats, presumably encodes a transposase of about 340 amino acids with a DDE motif, and has an amino-terminal domain with a strong nuclear localization function. Seven other transposable elements with homologous but distinct sequences were isolated from the B. mori genome. Together with plaque hybridization results, our findings suggest that these novel elements exist in multiple copies constituting a new Tc-like transposable element family in the silkworm genome.
Mikitani
K
K
Department of Biosciences at Novum, Karolinska Institute, Halsovägen 7, S-141 57 Huddinge, Sweden. kenichi.mikitani@csb.ki.se
Sugasaki
T
T
Shimada
T
T
Kobayashi
M
M
Gustafsson
J A
JA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000201:enzymology / Q000235:genetics*; D002688:Chitinases / Q000235:genetics*; D004251:DNA Transposable Elements; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
D004251:DNA Transposable Elements; D018076:DNA, Complementary; D002688:Chitinases