Packaging of tobacco mosaic virus subgenomic RNAs by Brome mosaic virus coat protein exhibits RNA controlled polymorphism.
The coat protein (CP) of icosahedral Brome mosaic virus (BMV) was expressed from a genetically engineered rod-shape Tobacco mosaic virus. Molecular characterization of the progeny recovered from symptomatic plants revealed that BMV CP selectively packaged the three subgenomic RNAs of the hybrid virus into two differently sized icosahedral virus-like particles (VLPs). The smaller VLPs packaged only the two smaller subgenomic RNAs. Additional in vitro reassembly assays with BMV CP subunits and transcripts of hybrid subgenomic RNAs further demonstrated that the ability of BMV capsids to display polymorphism is not dependent on the RNA size alone and appears to be controlled by some other feature(s) of the genetically engineered RNA.
Choi
Y G
YG
Department of Plant Pathology, University of California, Riverside, California 92521-0122, USA.
Rao
A L
AL
D000818:Animals; D015152:Blotting, Northern; D017795:Bromovirus / Q000378:metabolism*; D002213:Capsid / Q000378:metabolism*; D019663:Chenopodiaceae / Q000821:virology; D011110:Polymorphism, Genetic; D011485:Protein Binding; D012367:RNA, Viral / Q000378:metabolism*; D014027:Tobacco Mosaic Virus / Q000235:genetics*; D014771:Virion / Q000378:metabolism; D019065:Virus Assembly; D014981:Xenopus
D012367:RNA, Viral