In-vitro maturation of immature human male germ cells.
Both meiotic and postmeiotic maturation events have been observed to occur in human male germ cells during in-vitro culture. The temperature of 30 degrees C, medium supplementation with follicle-stimulating hormone and testosterone and the maintenance of the original cell-cell associations within explanted segments of the testicular seminiferous tubules are common features of the most efficient culture systems. The in-vitro maturation processes are markedly accelerated as compared to the in-vivo situation, probably due to the abrogation of a checkpoint controlling the full assembly of molecules needed for spermiogenesis. Moreover, both meiotic and postmeiotic maturation processes can be reactivated in vitro in some cases with a complete block of the same processes in vivo. Healthy babies were born after micromanipulation-assisted fertilization with in-vitro matured elongated spermatids from men with complete in-vivo maturation arrest at the round spermatid stage and at the primary spermatocyte stage.
Tesarik
J
J
Laboratoire d'Eylau, 55 rue Saint-Didier, 75116, Paris, France.
Mendoza
C
C
Greco
E
E
D002454:Cell Differentiation / Q000187:drug effects; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007231:Infant, Newborn; D007248:Infertility, Male / Q000473:pathology / Q000628:therapy; D008297:Male; D008540:Meiosis / Q000187:drug effects; D011247:Pregnancy; D012099:Reproductive Techniques; D013091:Spermatogenesis / Q000187:drug effects; D013094:Spermatozoa / Q000166:cytology* / Q000187:drug effects
D005640:Follicle Stimulating Hormone