Influence of arginine-8-vasotocin and indomethacin on in vitro progesterone secretion by porcine granulosa cells.
The effect of the nonapeptide hormone arginine-8-vasotocin (AVT) and indomethacin on progesterone (P) production by cultured granulosa cells (GCs) from porcine preovulatory follicles was investigated. AVT dose-dependently stimulated the release of P by GCs. Basal and AVT-stimulated P secretion was inhibited when cultures were pretreated with the cyclooxigenase inhibitor indomethacin. These results suggest that AVT is involved in the local control of porcine follicle functions and that the effects of AVT on the P release are dependent on the endogenous production of prostaglandins (PGs) in the GCs.
Denkova
R
Institute of Cell Biology and Morphology, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria.
Nikolov
A
Hristov
I