title: Molecular characterization and leukocyte distribution of a teleost beta1 integrin molecule.
abstract: The beta1 integrin, in combination with the alpha subunit, is responsible for migration of leukocytes into areas of inflammation. Although identified in mammalian species; the beta1 or CD29 molecule has yet to be identified in fish. The present investigation has identified a full-length channel catfish, Ictalurus punctatus, cDNA beta1 molecule composed of 2786 bases and a deduced amino acid sequence of 797 amino acids. The catfish molecule has an amino acid identity ranging from 71.87 to 74.12% with bovine, feline, human, and Xenopus. The channel catfish molecule retains several characteristics of mammalian beta1 molecules, such as four cysteine-rich repeat regions, and eight potential N-linked glycosylation sites. Based on Western blotting the channel catfish beta1 molecule has a molecular mass of approximately 130kDa, essentially the same as that for mammalian species. These results confirm the existence and expression of a beta1 gene in channel catfish, a species phylogenetically distant from mammals.
authors.affiliation: Department of Microbiology and Immunology, CB 7290, University of North Carolina, Chapel Hill, NC 27599, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Qian
authors.forename: M
authors.initials: M
authors.lastname: Noya
authors.forename: A J
authors.initials: AJ
authors.lastname: Ainsworth
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003400:Astacoidea / Q000235:genetics; D001483:Base Sequence; D015153:Blotting, Western / Q000662:veterinary; D002397:Catfishes / Q000235:genetics / Q000378:metabolism*; D002415:Cats; D002417:Cattle; D005434:Flow Cytometry / Q000662:veterinary; D006801:Humans; D019012:Integrin beta1 / Q000737:chemistry* / Q000235:genetics*; D007962:Leukocytes / Q000737:chemistry*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011487:Protein Conformation; D019105:Random Amplified Polymorphic DNA Technique / Q000662:veterinary; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D014981:Xenopus
chemical_list: D019012:Integrin beta1