XAGE-1, a new gene that is frequently expressed in Ewing's sarcoma.
Our previous expressed sequence tag database analysis indicates that XAGE-1 is frequently found in Ewing's sarcoma and alveolar rhabdomyosarcoma (U. Brinkmann et al., Cancer Res., 59: 1445-1448, 1999). Using Northern blots and RNA dot blots, we have now found that XAGE-1 is highly expressed in normal testis, in seven of eight Ewing's cell lines, in four of nine Ewing's sarcoma patient samples, and in one of one alveolar rhabdomyosarcoma patient sample. The gene is located on the X chromosome. The full-length cDNA contains 611 bp and predicts a protein of Mr 16,300 with a potential transmembrane domain at the NH2 terminus. XAGE-1 shares homology with GAGE/PAGE proteins in the COOH-terminal end. These findings could be valuable for cancer diagnosis and cancer immunotherapy.
Liu
X F
XF
Laboratory of Molecular Biology, Division of Basic Sciences, NIH, National Cancer Institute, Bethesda, Maryland 20892-4255, USA.
Helman
L J
LJ
Yeung
C
C
Bera
T K
TK
Lee
B
B
Pastan
I
I
D000595:Amino Acid Sequence; D000951:Antigens, Neoplasm / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D001859:Bone Neoplasms / Q000235:genetics / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D008297:Male; D012516:Osteosarcoma / Q000235:genetics / Q000378:metabolism; D010446:Peptide Fragments / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018232:Rhabdomyosarcoma, Alveolar / Q000235:genetics / Q000378:metabolism; D012512:Sarcoma, Ewing / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013737:Testis / Q000378:metabolism / Q000502:physiology; D014960:X Chromosome / Q000235:genetics
D000951:Antigens, Neoplasm; D018076:DNA, Complementary; D004273:DNA, Neoplasm; C113433:PAGE1 protein, human; D010446:Peptide Fragments; D012333:RNA, Messenger; C413656:XAGE1A protein, human