Co-localization of N-methyl-D-aspartate receptors and substance P (neurokinin-1) receptors in rat spinal cord.
Glutamate, substance P (SP), and their receptors have been implicated in the initiation and maintenance of persistent pain through an interaction at second order spinal cord neurons. Employing well-characterized antibodies to the SP receptor and the N-methyl-D-aspartate receptor (NR1 subunit, splice variant missing exon 22), we demonstrate co-localization of these receptors on second order neurons at cervical, thoracic, lumbar, and sacral spinal cord levels. The co-localization was marked in lamina I of the dorsal horn at all levels and in the intermediolateral nucleus of the thoraco-lumbar spinal cord nuclei associated with autonomic function.
Benoliel
R
R
Neuronal Gene Expression Unit, Pain and Neurosensory Mechanisms Branch, Building 49, Room 1A08, National Institute of Dental and Craniofacial Research, National Institutes of Health, 49 Convent Drive, MSC 4410, MD 20892-4410, Bethesda, USA.
Tanaka
M
M
Caudle
R M
RM
Iadarola
M J
MJ
D000595:Amino Acid Sequence; D000818:Animals; D006614:Hindlimb; D007150:Immunohistochemistry; D008297:Male; D008969:Molecular Sequence Data; D010523:Peripheral Nervous System Diseases / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D018040:Receptors, Neurokinin-1 / Q000378:metabolism*; D013116:Spinal Cord / Q000737:chemistry* / Q000378:metabolism* / Q000473:pathology
D016194:Receptors, N-Methyl-D-Aspartate; D018040:Receptors, Neurokinin-1