Analysis of the genetic determinant for production of the pediocin P of Pediococcus pentosaceus Pep1.
Pediococcus pentosaceus Pep1 is a vacuum-packaged Turkish sausage isolate which produces a potentially novel bacteriocin of the pediocin (anti-Listeria) family of peptides designated as pediocin P. Curing experiments and plasmid profile analysis indicated that both bacteriocin immunity and production determinants were linked and encoded by 9.0 MDa plasmid, pHD1.0. Attempts to transform purified plasmid pHD1.0 into recipient Escherichia coli JM109 cells by electroporation were successful but none of the E. coli JM109 cells were able to express and/or release pediocin P. However, P. pentosaceus PC, a plasmid-cured variant of P. pentosaceus Pep1 was successfully transformed with pHD1.0 by electroporation and Bac-Bacs P. pentosaceus PC cells restarted to express and/or release pediocin P again as indicated by the presence of zone of growth inhibition of L. plantarum NCDO 955 around colonies.
Osmanağaoğlu
O
O
Gazi University, Faculty of Arts and Science, Department of Biology, Turkey. ozlemos@quark.fef.gazi.edu.tr
Beyatli
Y
Y
Gündüz
U
U
Saçilik
S C
SC
D001430:Bacteriocins / Q000096:biosynthesis* / Q000494:pharmacology; D018274:Electroporation; D007778:Lactobacillus / Q000187:drug effects; D010376:Pediococcus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics*; D014169:Transformation, Bacterial / Q000235:genetics
D001430:Bacteriocins