Scale-down of continuous protein purification by annular chromatography. Design parameters for the smallest unit.
Theoretically, in fixed-bed chromatography an infinite separation time or volume is available. In annular chromatography the separation time is limited by the time required for a single rotation. In a chromatograph with a smaller annulus than the theoretical one, the equilibration zone will overtake the feed zone. The required separation time is determined by a number of steps such as equilibration, loading, washing, elution, regeneration and the retention of the solute. A mathematical model has been developed to estimate the minimum radius of an annular chromatograph. The minimal geometry of an annular chromatograph was calculated for a model system immunoglobulin and bovine serum albumin.
Uretschläger
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Institute of Applied Microbiology, University of Agricultural Sciences, Vienna, Austria.
Jungbauer
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D000418:Albumins / Q000032:analysis*; D002021:Buffers; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D006863:Hydrogen-Ion Concentration; D007136:Immunoglobulins / Q000032:analysis*
D000418:Albumins; D002021:Buffers; D007136:Immunoglobulins