[Studies on expression and function of human membrane cofactor protein in NIH3T3 cells].
One recombinant human membrane cofactor protein (MCP) expression plasmid pcDNA3 MCP was constructed. After transfected into NIH3T3 cells with calcium phosphate-DNA precipitate method, the expression clones were obtained by G418 selection. To determine whether MCP generated on heterologous cells by cDNA transfection retains its capacity to inhibit human complement, NIH3T3 cells transfected with MCP cDNA were incubated with human serum as well as guinea pig, rabbit serum. Under identical conditions, the control cells transfected with pcDNA3 vector were completely lysed by human serum but the cells transfected with MCP were barely lysed. However, the cells transfected with MCP were lysed by guinea pig and rabbit serum complement as the same extent as control cells.
Huang
J
J
College of Life Sciences, Wuhan University.
Li
W X
WX
Zhen
C Y
CY
D016475:3T3 Cells; D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis* / Q000502:physiology; D003165:Complement System Proteins / Q000502:physiology; D006168:Guinea Pigs; D006801:Humans; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000502:physiology; D051379:Mice; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011817:Rabbits; D011994:Recombinant Proteins / Q000096:biosynthesis*; D014162:Transfection
D015703:Antigens, CD; C497857:CD46 protein, human; C497859:Mcp protein, mouse; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; D003165:Complement System Proteins