Hypothalamo-pituitary-adrenal axis activation by administration of cyanamide: a potent inhibitor of aldehyde dehydrogenase.
In this report, we investigated the effects of cyanamide (a potent inhibitor of aldehyde dehydrogenase (ALDH: EC 1.2.1.3)) on hypothalamo-pituitary adrenal (HPA)-axis using in situ hybridization histochemistry and radioimmunoassay. Cyanamide administration resulted in a dose-dependent increase in plasma corticosterone concentrations, significant increases in not only corticotrophin releasing factor (CRF) mRNA, but also arginine vasopressin (AVP) mRNA in the paraventricular nucleus (PVN) and proopiomelanocortin (POMC) mRNA in the anterior pituitary. These results suggest that cyanamide is able to activate the HPA axis at all levels of the axis.
Kinoshita
H
H
Division of Medicine, University of Bristol, BRI, Marlborough Street, BS2 8HW, Bristol, UK. kinochin@kms.ac.jp
Harbuz
M S
MS
Jessop
D S
DS
Finn
D P
DP
Ameno
S
S
Ameno
K
K
Kubota
T
T
Ijiri
I
I
D000444:Aldehyde Dehydrogenase / Q000187:drug effects*; D000818:Animals; D001127:Arginine Vasopressin / Q000032:analysis / Q000097:blood; D001923:Brain Chemistry / Q000187:drug effects*; D003345:Corticosterone / Q000032:analysis / Q000097:blood; D003346:Corticotropin-Releasing Hormone / Q000032:analysis / Q000097:blood; D003484:Cyanamide / Q000009:adverse effects*; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D006651:Histocytochemistry; D007030:Hypothalamo-Hypophyseal System / Q000187:drug effects* / Q000201:enzymology*; D017403:In Situ Hybridization; D008297:Male; D010913:Pituitary-Adrenal System / Q000187:drug effects* / Q000201:enzymology*; D011333:Pro-Opiomelanocortin / Q000032:analysis / Q000097:blood; D011863:Radioimmunoassay; D051381:Rats; D017207:Rats, Sprague-Dawley
D001127:Arginine Vasopressin; D003484:Cyanamide; D011333:Pro-Opiomelanocortin; D003346:Corticotropin-Releasing Hormone; D000444:Aldehyde Dehydrogenase; D003345:Corticosterone