Yeast recombinase FLP functions effectively in human cells for construction of adenovirus vectors.
We have recently developed a high-efficiency method of constructing adenovirus vectors based on Cre-mediated recombination between two plasmids co-transfected into 293 cells. The simplicity and efficiency of this method should greatly expedite the construction of most recombinant vectors. However, this system would not be suitable for constructing vectors bearing loxP sites elsewhere in the genome because of undesirable Cre-mediated vector rearrangements. To address this, we have developed a similar system using FLP-mediated site-specific recombination for the construction of adenovirus vectors.
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McMaster University, Hamilton, ON, Canada.
Cummings
D T
DT
Evelegh
C M
CM
Graham
F L
FL
D000256:Adenoviridae / Q000235:genetics*; D002460:Cell Line; D004247:DNA / Q000235:genetics; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D005822:Genetic Vectors; D006801:Humans; D019426:Integrases / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D014162:Transfection; D014764:Viral Proteins; D001616:beta-Galactosidase / Q000235:genetics
D014764:Viral Proteins; D004247:DNA; C045073:Cre recombinase; D004254:DNA Nucleotidyltransferases; C045119:FLP recombinase; D019426:Integrases; D001616:beta-Galactosidase