title: Induction of membrane chloride currents in Xenopus laevis oocytes by the sulfonyl amide sweeteners acesulfame K and saccharin.
abstract: Sweet receptors have remained elusive. In Xenopus oocytes sulfonyl amide sweeteners but not sweet compounds belonging to other chemical classes dose dependently induced membrane chloride currents via the inositol trisphosphate/calcium pathway. Induction of membrane currents was exclusively observed following extracellular application of sulfonyl amides but not by intracellular pressure injection, suggesting the involvement of a plasma membrane receptor. The presence of this receptor in oocytes and the observed seasonal variation of the sweet response offers an opportunity for a molecular cloning approach.
authors.affiliation: Deutsches Institut für Ernährungsforschung, Abteilung Molekulare Genetik und Universität Potsdam, Potsdam-Rehbrücke, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Bufe
authors.forename: H
authors.initials: H
authors.lastname: Schmale
authors.forename: W
authors.initials: W
authors.lastname: Meyerhof
mesh_terms: D000818:Animals; D018118:Chloride Channels / Q000187:drug effects*; D005260:Female; D009865:Oocytes / Q000187:drug effects* / Q000378:metabolism; D012439:Saccharin / Q000494:pharmacology*; D013549:Sweetening Agents / Q000494:pharmacology*; D013843:Thiazines / Q000494:pharmacology*; D014982:Xenopus laevis
chemical_list: D018118:Chloride Channels; D013549:Sweetening Agents; D013843:Thiazines; D012439:Saccharin; C006362:acetosulfame