title: Effects of intrauterine IL-6 and IL-8 on the expression of surfactant apoprotein mRNAs in the fetal rat lung.
abstract: OBJECTIVE
  The purpose of this study was to investigate the effect of interleukin-6 (IL-6) and interleukin-8 (IL-8), whose concentrations are elevated with chorioamnionitis, on the expression of surfactant apoprotein mRNAs in fetal rat lung.
  
  
  METHODS
  We employed an animal model in which we were able to administer substances continuously into the cavity between the fetal membranes and endometrium using a miniosmotic pump. Lipopolysaccharide (LPS), IL-6, or IL-8 was administered to timed pregnant rats for 3 days (day 16-19), and fetal lung expression of surfactant apoprotein mRNAs for SP (surfactant apoprotein)-A, SP-B, and SP-C was evaluated by Northern blot hybridization.
  
  
  RESULTS
  Continuous administration of LPS increased the expression of each surfactant apoprotein mRNA, but the expression of mRNAs was not dose-dependent. On the other hand, continuous IL-6 or IL-8 administration increased the expression of each surfactant apoprotein mRNA in a dose-dependent manner.
  
  
  CONCLUSIONS
  Fetal lung maturation may be promoted by either IL-6 or IL-8 produced in response to chorioamnionitis.
authors.affiliation: Department of Obstetrics and Gynecology, Akita University School of Medicine, 1-1-1 Hondo, 010, Akita, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ikegami
authors.forename: A
authors.initials: A
authors.lastname: Tsuda
authors.forename: A
authors.initials: A
authors.lastname: Karube
authors.forename: H
authors.initials: H
authors.lastname: Kodama
authors.forename: H
authors.initials: H
authors.lastname: Hirano
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D005260:Female; D015870:Gene Expression; D006801:Humans; D015850:Interleukin-6 / Q000008:administration & dosage* / Q000494:pharmacology; D016209:Interleukin-8 / Q000008:administration & dosage* / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000494:pharmacology; D008168:Lung / Q000196:embryology* / Q000378:metabolism; D011247:Pregnancy; D011510:Proteolipids / Q000235:genetics; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D015850:Interleukin-6; D016209:Interleukin-8; D008070:Lipopolysaccharides; D011510:Proteolipids; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants; D012333:RNA, Messenger