A novel protein (Fbf-1) that binds to CD95/APO-1/FAS and shows sequence similarity to trichohyalin and plectin.
The Fas/Apo-1/CD95 cell surface receptor belongs to the TNF receptor family of cell death inducing molecules. A number of cytosolic adapter proteins that mediate signal transduction of CD95 have been characterized, but some features of the molecular mechanisms of CD95-induced cell death remain elusive. We describe here a novel protein that can interact with the cytosolic domain of the murine CD95 receptor in a yeast two-hybrid assay. This novel protein was termed Fbf-1 for Fas binding factor and bears no sequence similarity to the known CD95 adapter proteins. Fbf-1 is 1173 aa long and has a theoretical molecular weight of around 130 kDa. The protein is expressed in a wide variety of tissues and is localized in the cytoplasm. Fbf-1 is a very hydrophilic protein, highly conserved between mouse and human and bears a carboxyterminal leucine heptad repeat reminiscent of leucine zipper protein interaction domains. In addition, it shows sequence similarity to trichohyalin and plectin pointing to a function as a structural protein.
Schmidt
T
T
Institut für Zellbiologie, IFZ, Universitätsklinikum Essen, Virchowstrasse 173, D-45122, Essen, Germany.
Karsunky
H
H
Frass
B
B
Baum
W
W
Denzel
A
A
Möröy
T
T
D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D019677:Artificial Gene Fusion; D002352:Carrier Proteins; D003593:Cytoplasm / Q000737:chemistry; D015723:Gene Library; D006801:Humans; D007381:Intermediate Filament Proteins / Q000737:chemistry*; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism; D051190:Plectin; D017354:Point Mutation; D020033:Protein Isoforms / Q000737:chemistry; D011498:Protein Precursors / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid; D013737:Testis / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry*; D015003:Yeasts / Q000235:genetics; D019014:fas Receptor / Q000737:chemistry*
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; C095245:FBF1 protein, human; D007381:Intermediate Filament Proteins; C495332:PLEC protein, human; C495333:Plec protein, mouse; D051190:Plectin; D020033:Protein Isoforms; D011498:Protein Precursors; D014157:Transcription Factors; D019014:fas Receptor; C016159:trichohyalin