Development of the primer set for the detection of the hsp60 gene in Trichophyton mentagrophytes cDNA.
Three sequences of hsp60 from Saccharomyces cerevisiae, Schizosaccharomyces pombe and Histoplasma capsulatum were compared. Local multiple alignment of these sequences allowed the selection of two oligonucleotides suitable as primers for the polymerase chain reaction. This primer set was used for the amplification of a part of the hsp60 gene from cDNA of Trichophyton mentagrophytes and S. cerevisiae. Similar fragments detected in both PCR's imply the possible future use of the developed primer set for the detection of the hsp60 gene in other fungal species.
Kopecek
P
P
Department of Biology, Medical Faculty, Palacký University, Olomouc, Czech Republic. kopecek@tunw.upol.cz
Raska
M
M
Weigl
E
E
D001483:Base Sequence; D018834:Chaperonin 60 / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics*; D005800:Genes, Fungal; D006658:Histoplasma / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012568:Schizosaccharomyces / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014249:Trichophyton / Q000235:genetics*
D018834:Chaperonin 60; D017931:DNA Primers; D018076:DNA, Complementary; D004271:DNA, Fungal