Enhancement of DNA vaccine potency by electroporation in vivo.
The potential of electric current-mediated delivery technology to enhance DNA delivery and DNA vaccine potency was evaluated. Higher levels of reporter gene expression were observed in muscle cells of mice inoculated with luciferase or beta-galactosidase DNA followed by the application of electrical current, compared with DNA injected with no current. Similarly, substantially higher levels of immune responses (up to 20-fold) were demonstrated in mice vaccinated with HIV gag DNA and electric current. These enhanced responses were observed after one or two inoculations, and were maintained for at least 12 weeks. Therefore, the present studies demonstrate the utility of electroporation for enhancement of DNA vaccine potency in animals.
Selby
M
M
Vaccines Research, Chiron Corporation, 4560 Horton Street, Emeryville, CA 94608, USA.
Goldbeck
C
C
Pertile
T
T
Walsh
R
R
Ulmer
J
J
D000818:Animals; D018274:Electroporation; D005260:Female; D015683:Gene Products, gag / Q000276:immunology; D051379:Mice; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology
D015683:Gene Products, gag; D019444:Vaccines, DNA