Binding of glycoglycerolipid derived from membranes of Acholeplasma laidlawii PG8 and synthetic analogues to lymphoid cells.
A component that binds to human lymphoid cells was isolated from the membranes of Acholeplasma laidlawii PG8. The component was extracted using the Bligh-Dyer method and purified using a silica-gel column and TLC. The active component was identified as 3-O:-[2'-O-(alpha-D-glucopyranosyl)- 6'-O-acyl-alpha-D-glucopyranosyl]-1,2-di-O- acyl-sn-glycerol (GAGDG) using (1)H- and (13)C-NMR and GC-MS. The compositions of the major saturated fatty acids were nC (14) (17.8%), isoC(14) (10.7%) and nC (16) (34.9%) as determined by GC-MS. The amounts of unsaturated species were less than 10% of those of the corresponding saturated acids. GAGDGs which have three tetradecanoyl groups were synthesized. These synthetic GAGDGs, as well as GAGDGs derived from A. laidlawii membranes, had a high binding affinity for MOLT-4 and HUT-78 (human T cell lines), Raji (a B cell line), HL-60 (a monoblastoid cell line) and primary cultured human T cells. The binding affinities of GAGDGs with an isoC(14) acyl group was higher than those with nC(14) and nC(16) acyl groups. The binding to lymphoid cells reveals a novel biological activity of GAGDGs.
Toujima
Saori
S
Department of Bacteriology, Kurume University School of Medicine, 67 Asahi-machi, Kurume 830-0011, Japan1.
Kuwano
Koichi
K
Department of Bacteriology, Kurume University School of Medicine, 67 Asahi-machi, Kurume 830-0011, Japan1.
Zhang
Ye
Y
Department of Bacteriology, Kurume University School of Medicine, 67 Asahi-machi, Kurume 830-0011, Japan1.
Fujimoto
Naoyuki
N
Department of Bacteriology, Kurume University School of Medicine, 67 Asahi-machi, Kurume 830-0011, Japan1.
Hirama
Masahiro
M
Department of Chemistry, Graduate School of Science, Tohoku University Aoba, Aramaki aza, Aoba-ku, Sendai 980-8578, Japan2.
Oishi
Tohru
T
Department of Chemistry, Graduate School of Science, Tohoku University Aoba, Aramaki aza, Aoba-ku, Sendai 980-8578, Japan2.
Fukuda
Sumiko
S
Department of Chemistry, Graduate School of Science, Tohoku University Aoba, Aramaki aza, Aoba-ku, Sendai 980-8578, Japan2.
Nagumo
Yoko
Y
Department of Chemistry, Graduate School of Science, Tohoku University Aoba, Aramaki aza, Aoba-ku, Sendai 980-8578, Japan2.
Imai
Hiroto
H
Department of Chemistry, Graduate School of Science, Tohoku University Aoba, Aramaki aza, Aoba-ku, Sendai 980-8578, Japan2.
Kikuchi
Tsukasa
T
Department of Chemistry, Graduate School of Science, Tohoku University Aoba, Aramaki aza, Aoba-ku, Sendai 980-8578, Japan2.
Arai
Sumio
S
Department of Bacteriology, Kurume University School of Medicine, 67 Asahi-machi, Kurume 830-0011, Japan1.
D000128:Acholeplasma laidlawii / Q000737:chemistry*; D001422:Bacterial Adhesion; D002240:Carbohydrate Sequence; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry; D006017:Glycolipids / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D007963:Leukocytes, Mononuclear; D008214:Lymphocytes / Q000378:metabolism*; D008969:Molecular Sequence Data
D006017:Glycolipids