Determination of total taurine in pet foods by liquid chromatography of the dansyl derivative: collaborative study.
A liquid chromatographic (LC) method for the determination of total taurine in pet foods was evaluated in a collaborative study. Ten laboratories assayed 6 blind duplicate pairs of wet and dry pet foods. The taurine in the 6 sample pairs ranged from low (170 mg/kg) to high (2250 mg/kg) concentrations as is. Collaborators also assayed a sample of known taurine concentration for familiarization purposes. Samples were hydrolyzed to release bound taurine, which was subsequently converted to the dansyl derivative and quantitated by gradient-elution LC with fluorescence detection. Repeatability relative standard deviations, RSDr, ranged from 3.2 to 10.0%; reproducibility relative standard deviations, RSDR, ranged from 6.1 to 16.1%. The method has been adopted Official First Action status by AOAC INTERNATIONAL.
McCarthy
K
K
Pedigree Masterfoods, Melton Mowbray, Leicestershire, United Kingdom.
Hischenhuber
C
C
Joyce
N
N
McCarthy
K
K
Hischenhuber
C
C
Joyce
N
N
D000818:Animals; D000829:Animals, Domestic; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D003619:Dansyl Compounds; D005504:Food Analysis / Q000379:methods*; D006868:Hydrolysis; D007753:Laboratories; D016014:Linear Models; D015203:Reproducibility of Results; D013654:Taurine / Q000032:analysis*
D003619:Dansyl Compounds; D013654:Taurine