Design and synthesis of triglyceride analogue biotinylated suicide inhibitors for directed molecular evolution of lipolytic enzymes.
The design, synthesis, and inhibition properties of two new triglyceride analogue biotinylated suicide inhibitors (2) and (3) for directed molecular evolution of lipolytic enzymes by phage-display is described.
Deussen
H J
HJ
Protein Discovery, Novo Nordisk A/S, Bagsvaerd, Denmark. hjdu@novo.dk
Danielsen
S
S
Breinholt
J
J
Borchert
T V
TV
D000345:Affinity Labels; D019921:Biotinylation; D015195:Drug Design; D004791:Enzyme Inhibitors / Q000138:chemical synthesis* / Q000494:pharmacology; D008049:Lipase / Q000037:antagonists & inhibitors*; D014280:Triglycerides / Q000138:chemical synthesis* / Q000494:pharmacology
D000345:Affinity Labels; D004791:Enzyme Inhibitors; D014280:Triglycerides; D008049:Lipase