Liquid chromatography-electrospray tandem mass spectrometry of acidic monoamine metabolites.
A new method based on liquid chromatography-tandem mass spectrometry has been developed for the determination of monoamine metabolites, i.e., homovanillic acid (HVA), vanilmandelic acid (VMA), 3,4-dihydroxyphenylacetic acid (DOPAC) and 5-hydroxyindoleacetic acid (5-HIAA) in human urine. Analytes were separated on a C16 amide (5 cm, 5 microm) column and ionized by negative ion electrospray. Operating in the selected-reaction monitoring mode, linearity was established over three-orders of magnitude and limits of detection were in the range 30-70 microg/l. Precision calculated as RSD was within 0.8-5.2% for all intra- and inter-day determinations. The method was applied to the quantitative analysis of monoamine metabolites in 700 urine samples from occupationally (adults) and environmentally (both children and adults) exposed people living in areas with different soil contamination from lead. The urinary excretion of monoamine metabolites was significantly higher (P<0.001) in the subgroup of children living in polluted areas as compared to the control group (HVA, 6.03 vs. 4.57 mg/g creatinine; VMA, 5.33 vs. 4.37 mg/g creatinine; 5-HIAA 3.24 vs. 2.45 mg/g creatinine). In adults belonging to both groups of subjects occupationally and environmentally exposed, no differences were detected in the urinary concentration of monoamine metabolites. However, adults showed lower values of HVA (2.57 mg/g creatinine), VMA (2.17 mg/g creatinine) and 5-HIAA (2.09 mg/g creatinine) as compared to children groups.
Manini
P
P
Dipartimento di Clinica Medica, Nefrologia e Scienze della Prevenzione, Università degli Studi di Parma, Italy. medlav@unipr.it
Andreoli
R
R
Cavazzini
S
S
Bergamaschi
E
E
Mutti
A
A
Niessen
W M
WM
D000328:Adult; D015306:Biogenic Monoamines / Q000652:urine*; D002648:Child; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D007855:Lead Poisoning / Q000652:urine; D013058:Mass Spectrometry / Q000379:methods*; D016273:Occupational Exposure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D015306:Biogenic Monoamines