title: Yarrowia lipolytica SRP receptor alpha-subunit.
abstract: The Yarrowia lipolytica SRP101 homologue encoding the signal recognition particle (SRP) receptor alpha-subunit (SRalphap) was cloned using degenerate primers designed for conserved GTP-binding domains. Sequencing of 2814 nucleotides revealed an open reading frame of 1671 base pairs encoding a putative protein of 557 amino acids with a predicted molecular mass of 61 kDa. Like other SRP101 homologues, Y. lipolytica SRP101 contains a highly conserved C-terminal GTP binding site. It has 44%, 34% and 22% sequence identity with S. cerevisiae, mammalian and Escherichia coli homologues, respectively. As found for SRP protein subunits of Y. lipolytica, SRP101 is important but not essential for cell growth. A conditional mutation in SRP101 affected synthesis/translocation of alkaline extracellular protease and Kar2p consistent with Srp101p functioning as an SRP receptor subunit. The SRP101 sequence has been deposited in GenBank under Accession No. AF132597.
authors.affiliation: Institute of Marine Resources, University of California, One Shields Avenue, Davis, CA 95616, USA.
authors.forename: K
authors.initials: K
authors.lastname: Kim
authors.forename: D M
authors.initials: DM
authors.lastname: Ogrydziak
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001665:Binding Sites / Q000235:genetics; D001692:Biological Transport / Q000235:genetics; D002455:Cell Division / Q000187:drug effects; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism; D020555:GTP-Binding Protein Regulators / Q000235:genetics*; D019204:GTP-Binding Proteins / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D021122:Protein Subunits; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics*; D018000:Receptors, Peptide / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases / Q000378:metabolism; D015003:Yeasts / Q000235:genetics* / Q000254:growth & development
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; D020555:GTP-Binding Protein Regulators; D018840:HSP70 Heat-Shock Proteins; C060153:KAR2 protein, yeast; D021122:Protein Subunits; D018160:Receptors, Cytoplasmic and Nuclear; D018000:Receptors, Peptide; C417984:SRP101 protein, Yarrowia lipolytica; C036318:signal peptide receptor; D012697:Serine Endopeptidases; C067468:Yarrowia lipolytica alkaline extracellular protease; D019204:GTP-Binding Proteins