Expression of collagen type I, II and III in loose body of osteoarthritis.
The expression of collagen type I, II, and III was investigated to evaluate phenotypic change in chondrocytes in loose bodies related to osteoarthritis. We assessed collagen type I, II, and III production in loose bodies from knee joints of ten osteoarthritic patients, using an immunohistochemical method with monoclonal antibodies. Collagen type III expression was identified in all ten loose bodies and was mainly located in cartilage, including chondrocytes and matrices, as well as in a layer of fibroid tissue on the surface. No positive signal for collagen type III was observed in necrotic osteocytes. There was weakly positive staining for collagen type I in chondrocytes. No positive staining for collagen type II could be seen in the cartilage of loose bodies. Cartilage from the non-osteoarthritic knee joints of four people was negative for the expression of collagen type I and III, and positive for the expression of collagen type II. Collagen type I and III expression suggested the dedifferentiation status of chondrocytes in loose bodies.
Pei
M
M
Division of Health Sciences and Technology, Massachusetts Institute of Technology, E25-342, 45 Carleton Street, Cambridge, MA 02139, USA.
Yu
C
C
Qu
M
M
D000328:Adult; D002358:Cartilage, Articular / Q000473:pathology; D016923:Cell Death / Q000502:physiology; D019902:Chondrocytes / Q000473:pathology; D003094:Collagen / Q000032:analysis*; D005260:Female; D006801:Humans; D007594:Joint Loose Bodies / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D020370:Osteoarthritis, Knee / Q000473:pathology*; D010011:Osteocytes / Q000473:pathology
D003094:Collagen