Polyclonal-antibody-mediated insolubilization and stabilization of papain.
Antisera raised in rabbits against native iodoacetamide- and iodoacetic acid-modified papain contained precipitating and non-inhibitory antibodies. Papain could be insolubilized as enzyme-antibody adducts with the gamma-globulin fraction derived from the antiserum. Two insolubilized papain preparations, designated A and B, with low and high antibody/enzyme ratios, respectively, exhibited high enzyme activity and markedly enhanced thermal and denaturant stabilities. However, papain antibody adduct B was superior in activity and stability over preparation A. The usefulness of immobilized papain in biopharmaceutical and other industries is also discussed.
Khan
S A
SA
Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh-202 002 (U.P.), India.
Iqbal
J
J
D000818:Animals; D000906:Antibodies / Q000378:metabolism*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry; D007074:Immunoglobulin G / Q000378:metabolism; D019807:Iodoacetic Acid / Q000737:chemistry; D010206:Papain / Q000737:chemistry* / Q000276:immunology* / Q000378:metabolism; D011233:Precipitin Tests; D011817:Rabbits; D012995:Solubility; D013696:Temperature
D000906:Antibodies; D004800:Enzymes, Immobilized; D007074:Immunoglobulin G; D010206:Papain; D019807:Iodoacetic Acid