Analysis of the co-amplified STR loci D1S1656, D12S391 and D18S51: population data and validation study for a highly discriminating triplex-PCR.
A multiplex-PCR composed of the three highly variable STR loci D1S1656, D12S391 and D18S51 has been established. The non-overlapping fragment sizes allow allele detection using a monochrome automated laser fluorescent sequencer (A.L.F. express, Pharmacia Biotech). The typing results of the triplex-PCR showed no difference to those of singleplex-PCR. Allele frequencies were determined in a Western German population of 228 individuals from Cologne. The heterozygosities and exclusion chances (D1S1656, 0.982; D12S391, 0.979; D18S51, 0.97) are very high compared to other short tandem repeats used for forensic applications. No deviations from the Hardy-Weinberg equilibrium were found. Successful typing of DNA amounts down to 50-100 pg is possible. Mixtures of up to 1:10 can be identified. In conclusion, the high combined exclusion chance due to the well-balanced allelic distribution and its high sensitivity make this triplex-PCR a valuable tool for forensic casework.
Schröer
K
K
Institut für Rechtsmedizin, Universität zu Köln, Melatengürtel 60-62, D-50823, Köln, Germany. a2417464@smail.uni-koeln.de
Schmitt
C
C
Staak
M
M
D001801:Blood Stains; D016172:DNA Fingerprinting / Q000379:methods*; D016002:Discriminant Analysis; D005787:Gene Frequency / Q000235:genetics*; D006580:Genetic Carrier Screening; D005858:Germany; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D018598:Minisatellite Repeats / Q000235:genetics*; D009010:Monte Carlo Method; D010334:Paternity; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity