Glial somatostatin-14 expression in the rat pituitary intermediate lobe: a possible neurotrophic function during development?
Somatostatin-14 was first detected on gestational day 17 in radially-oriented, bipolar cells spanning the width of the intermediate lobe of the rat pituitary. Cells were prominent, and constituted approximately 50% of the lobe area. The presence of vimentin, the cellular shape, and the localization identified these cells as glia. At postnatal day 6, somatostatin-14 and vimentin staining appeared in stellate-shaped cells. This is in agreement with the change from bipolar to stellate shape these glia undergo after the onset of innervation ([13] Gary et al. Int. J. Devl. Neurosci. 13, 555-565, 1995). Glia were more abundant, relative to melanotropes, throughout embryonic and early postnatal development compared to adulthood. Reverse transcription-polymerase chain reaction data showed a high level of prosomatostatin mRNA in the intermediate lobe, compared to the anterior and neural lobes from postnatal day 2 animals, and a significant drop in intermediate lobe content in the adult. The correlation between the number of glia and high expression of somatostatin in neonatal relative to adult tissue, together with the close apposition of incoming axons to the abundant, radially oriented glia during innervation of the lobe, support a neurotrophic function of glia-derived somatostatin.
Chronwall
B M
BM
School of Biological Sciences, University of Missouri-Kansas City, 5100 Rockhill Road, Kansas City, MO 64110, USA. chronwall@umkc.edu
Sands
S A
SA
Cummings
K C
KC
Schwartz
J P
JP
D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D001369:Axons / Q000502:physiology / Q000648:ultrastructure; D005260:Female; D007150:Immunohistochemistry; D009457:Neuroglia / Q000378:metabolism* / Q000648:ultrastructure; D010902:Pituitary Gland / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013004:Somatostatin / Q000096:biosynthesis* / Q000502:physiology*
D012333:RNA, Messenger; D013004:Somatostatin