title: Capacitance cytometry: measuring biological cells one by one.
abstract: Measuring the DNA content of eukaryotic cells is a fundamental task in biology and medicine. We have observed a linear relationship between the DNA content of eukaryotic cells and the change in capacitance that is evoked by the passage of individual cells across a 1-kHz electric field. This relationship is species-independent; consequently, we have developed a microfluidic technique-"capacitance cytometry"-that can be used to quantify the DNA content of single eukaryotic cells and to analyze the cell-cycle kinetics of populations of cells. Comparisons with standard flow cytometry demonstrate the sensitivity of this new technique.
authors.affiliation: Departments of Physics and Molecular Biology, Princeton University, Princeton, NJ 08544, USA. sohn@princeton.edu
authors.forename: L L
authors.initials: LL
authors.lastname: Sohn
authors.forename: O A
authors.initials: OA
authors.lastname: Saleh
authors.forename: G R
authors.initials: GR
authors.lastname: Facer
authors.forename: A J
authors.initials: AJ
authors.lastname: Beavis
authors.forename: R S
authors.initials: RS
authors.lastname: Allan
authors.forename: D A
authors.initials: DA
authors.lastname: Notterman
mesh_terms: D000818:Animals; D003584:Cytological Techniques; D004247:DNA / Q000032:analysis*; D006801:Humans; D055672:Static Electricity
chemical_list: D004247:DNA