Cloning and sequencing analysis of Trp1 gene of Flammulina velutipes.
The genomic TRP1 gene from basidiomycete Flammulina velutipes was cloned by complementation of yeast Saccharomyces cerevisiae trp1 mutation. Sequencing analysis revealed that the TRP1 gene encoded a single protein consisting of three catalytic functional domains; glutamine amidotransferase, indole-3-glycerol phosphate synthase ) and N-(5'-phosphoribosyl) anthranilate isomerase, in order of NH2-glutamine amidotransferase-indole-3-glycerol phosphate synthase N-(5'-phosphoribosyl) anthranilate isomerase-COOH. The coding sequence of the TRP1 gene was interrupted by a single intron of 48 bases, the position and flanking sequences of which were highly homologous to those of basidiomycete Phanerochaete chrysosporium trpC.
Nakai
R
R
Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University, Nagano, Japan.
Sen
K
K
Kurosawa
S
S
Shibai
H
H
D000363:Agaricales / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D019747:Aldose-Ketose Isomerases; D000595:Amino Acid Sequence; D000878:Anthranilate Synthase / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005822:Genetic Vectors; D007438:Introns; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D029701:Saccharomyces cerevisiae Proteins; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014170:Transformation, Genetic
D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; D000878:Anthranilate Synthase; D019747:Aldose-Ketose Isomerases; C490353:TRP1 protein, S cerevisiae