The growth of single roots of Artemisia annua in nutrient mist reactors.
To better characterize the development and growth of hairy roots in a mist-fed root bed, a single root aerosol reactor was developed. Growth kinetics studies were conducted on hairy roots of Artemisia annua as a function of the mist cycle, carrier gas, and nutrient compositions. Sustained rapid growth was only observed when conditioned medium was fed to the roots. The presence of 1% CO(2) in the carrier gas did not enhance the growth kinetics but it did prevent necrosis of the tissue at the highest mist cycle.
Wyslouzil
B E
BE
Department of Chemical Engineering, Worcester Polytechnic Institute, 100 Institute Road, Worcester, Massachusetts 01609-2280, USA. barbaraw@wpi.edu
Waterbury
R G
RG
Weathers
P J
PJ
D019306:Artemisia / Q000502:physiology*; D019149:Bioreactors; D002245:Carbon Dioxide / Q000494:pharmacology; D017077:Culture Media, Conditioned; D007700:Kinetics; D009336:Necrosis; D010946:Plants, Medicinal; D013997:Time Factors
D017077:Culture Media, Conditioned; D002245:Carbon Dioxide