title: Simultaneous detection of different glycosidase activities by 19F NMR spectroscopy.
abstract: A fast method for the simultaneous detection of different glycosidolytic activities in commercially available enzyme preparations and crude culture filtrates was found in using, as substrate, a mixture of different glycosyl fluorides and 19F NMR spectroscopy as a screening technique. Accompanying studies regarding the hydrolytic stability of these fluorides in various buffer systems, as well as conditions of their long-term storage, were carried out. A simple procedure for the preparation of beta-D-mannopyranosyl fluoride in gram quantities is given.
authors.affiliation: Institute of Organic Chemistry, Technical University, Graz, Austria.
authors.forename: M
authors.initials: M
authors.lastname: Albert
authors.forename: W
authors.initials: W
authors.lastname: Repetschnigg
authors.forename: J
authors.initials: J
authors.lastname: Ortner
authors.forename: J
authors.initials: J
authors.lastname: Gomes
authors.forename: B J
authors.initials: BJ
authors.lastname: Paul
authors.forename: C
authors.initials: C
authors.lastname: Illaszewicz
authors.forename: H
authors.initials: H
authors.lastname: Weber
authors.forename: W
authors.initials: W
authors.lastname: Steiner
authors.forename: K
authors.initials: K
authors.lastname: Dax
mesh_terms: D005461:Fluorine / Q000737:chemistry / Q000378:metabolism; D005462:Fluorine Radioisotopes; D006026:Glycoside Hydrolases / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods
chemical_list: D005462:Fluorine Radioisotopes; D005461:Fluorine; D006026:Glycoside Hydrolases