title: Sequence polymorphism, predicted secondary structures, and surface-exposed conformational epitopes of Campylobacter major outer membrane protein.
abstract: The major outer membrane protein (MOMP), a putative porin and a multifunction surface protein of Campylobacter jejuni, may play an important role in the adaptation of the organism to various host environments. To begin to dissect the biological functions and antigenic features of this protein, the gene (designated cmp) encoding MOMP was identified and characterized from 22 strains of C. jejuni and one strain of C. coli. It was shown that the single-copy cmp locus encoded a protein with characteristics of bacterial outer membrane proteins. Prediction from deduced amino acid sequences suggested that each MOMP subunit consisted of 18 beta-strands connected by short periplasmic turns and long irregular external loops. Alignment of the amino acid sequences of MOMP from different strains indicated that there were seven localized variable regions dispersed among highly conserved sequences. The variable regions were located in the putative external loop structures, while the predicted beta-strands were formed by conserved sequences. The sequence homology of cmp appeared to reflect the phylogenetic proximity of C. jejuni strains, since strains with identical cmp sequences had indistinguishable or closely related macrorestriction fragment patterns. Using recombinant MOMP and antibodies recognizing linear or conformational epitopes of the protein, it was demonstrated that the surface-exposed epitopes of MOMP were predominantly conformational in nature. These findings are instrumental in the design of MOMP-based diagnostic tools and vaccines.
authors.affiliation: Food Animal Health Research Program, The Ohio State University, Wooster, Ohio 44691, USA. zhang.234@osu.edu
authors.forename: Q
authors.initials: Q
authors.lastname: Zhang
authors.forename: J C
authors.initials: JC
authors.lastname: Meitzler
authors.forename: S
authors.initials: S
authors.lastname: Huang
authors.forename: T
authors.initials: T
authors.lastname: Morishita
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology; D001426:Bacterial Proteins; D002169:Campylobacter Infections / Q000382:microbiology*; D016123:Campylobacter jejuni / Q000737:chemistry* / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D002417:Cattle; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D008969:Molecular Sequence Data; D018272:Porins / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000276:immunology; D017422:Sequence Analysis, DNA
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D000939:Epitopes; D018272:Porins; D011994:Recombinant Proteins; C095080:major outer membrane protein, Campylobacter jejuni