Hyphenated HPLC-NMR and its applications in drug discovery.
Hyphenated HPLC-NMR is a fast growing technology, allowing rapid and detailed structural characterization of unknown mixtures. The technical aspects of the technology are reviewed on the basis of system configuration, operation, solvent suppression, HPLC and NMR optimization, and detection. The combined use of HPLC-NMR and HPLC-MS is also described and discussed. Various applications of HPLC-NMR and integrated HPLC-NMR-MS in drug discovery, especially in the separation and structure elucidation of drug impurities, reaction mixtures, degradation products, in vitro and in vivo metabolites, and combinatorial library samples, are illustrated.
Peng
S X
SX
The Procter & Gamble Company, Health Care Research Center, 8700 Mason-Montgomery Road, Mason, OH 45040, USA. peng.sx@pg.com
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D005544:Forecasting; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D011786:Quality Control; D012997:Solvents / Q000737:chemistry
D004364:Pharmaceutical Preparations; D012997:Solvents