Serotype-cross-reactive immunoglobulin M responses in dengue virus infections determined by enzyme-linked immunosorbent assay.
We developed immunoglobulin M (IgM) antibody capture enzyme-linked immunosorbent assays (ELISAs) with four monovalent dengue virus antigens. We attempted to determine whether IgM responses in dengue virus infections are serotype specific or serotype cross-reactive. Serum samples from 14 confirmed dengue cases were examined. In these 14 cases, which consisted of 12 Japanese and 2 non-Japanese patients, infecting dengue virus serotypes were defined by reverse transcription-PCR. Thirteen of the 14 cases were IgM positive in ELISA. IgM responses were serotype cross-reactive in these 13 cases but were highest against infecting dengue virus serotype in 9 of the 13 cases. These results indicate that IgM responses are generally dengue serotype cross-reactive but that IgM levels are highest against the infecting serotype in most dengue cases.
Nawa
M
M
Department of Microbiology, Saitama Medical School, Saitama 350-0495, Japan. mnawa@saitama-med.ac.jp
Yamada
K I
KI
Takasaki
T
T
Akatsuka
T
T
Kurane
I
I
D000330:Aedes; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology; D002460:Cell Line; D003429:Cross Reactions; D003715:Dengue / Q000097:blood / Q000175:diagnosis / Q000276:immunology*; D003716:Dengue Virus / Q000145:classification / Q000235:genetics / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood / Q000276:immunology*; D011933:Reagent Kits, Diagnostic; D012703:Serotyping
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007075:Immunoglobulin M; D011933:Reagent Kits, Diagnostic