title: Vacuolar-type H+ -ATPase distribution in unstimulated and acetylcholine-activated isolated human eccrine sweat glands.
abstract: The presence and cellular distribution of subunits of the V1 sector of the vacuolar-type H+ -ATPase (V-ATPase) was investigated in isolated human eccrine sweat glands. In every instance, V-ATPase was located in the cytoplasm and apical membranes of the luminal cells of the reabsorptive duct segment. In the secretory coil, both diffuse and perinuclear staining was demonstrated in the secretory cells, with additional expression at the apical and basolateral membranes and on the intercellular canaliculi. There was no detectable difference in V-ATPase expression as a result of prior application of 100 microM acetylcholine.
authors.affiliation: School of Biological and Biomedical Sciences, Glasgow Caledonian University, UK.
authors.forename: D L
authors.initials: DL
authors.lastname: Bovell
authors.forename: M T
authors.initials: MT
authors.lastname: Clunes
authors.forename: E
authors.initials: E
authors.lastname: Roussa
authors.forename: J
authors.initials: J
authors.lastname: Burry
authors.forename: H Y
authors.initials: HY
authors.lastname: Elder
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D019138:Antibodies, Blocking / Q000494:pharmacology; D004439:Eccrine Glands / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D018397:Equidae / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D014617:Vacuoles / Q000201:enzymology*
chemical_list: D019138:Antibodies, Blocking; D006180:Proton-Translocating ATPases; D000109:Acetylcholine