High-performance liquid chromatographic analysis for the determination of domperidone in human plasma.
A specific and sensitive high-performance liquid chromatographic method for the determination of domperidone in human plasma is described. Domperidone was isolated by solid-phase extraction using nitrile SPE cartridges. The drug was eluted with a mixture consisting of methanol-triethylamine-acetic acid, separated on a reversed-phase column, and measured by fluorimetric detection after post-column photoderivatization. The absolute extraction recovery from plasma samples was 83%. The limit of quantitation was established as 1 ng/ml. The relative standard deviation of the determination of plasma levels by this method over the standard curve concentration range was less than 10%, expect with the concentration of 1 ng/ml. The suitability of the method is shown for pharmacokinetic studies.
Kobylińska
M
M
Department of Pharmacology, Pharmaceutical Research Institute, Warsaw, Poland.
Kobylińska
K
K
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004294:Domperidone / Q000097:blood*; D018492:Dopamine Antagonists / Q000097:blood*; D006801:Humans; D012015:Reference Standards
D018492:Dopamine Antagonists; D004294:Domperidone