Expression of adhesion molecules in skin wounds: diagnostic value in legal medicine.
The adhesion molecules identified in recent years can help improve the diagnosis of the wound age, especially of injuries with a short survival time. This is also indicative of the vitality of the wounds. The material investigated in the study originated from 465 skin wounds. The samples were taken from human autopsy material, during the surgical treatment of wounds (excision) of patients and from experimental incised wounds of mice. To judge the age of skin wounds the endothelial adhesion molecules were detected in paraffin sections after autoclaving and using the ABC technique. Human skin wounds: strong positive staining was observed of ICAM-1 1.5 h at the earliest and 3.5 days at the latest, for the P-selectin 3 min at the earliest and 7 h at the latest, for the E-selectin 1 h at the earliest and 17 days at the latest and for VCAM-1 3 h at the earliest and 3.5 days at the latest after the time of injury. The L-selectin was expressed constitutively. Mice skin wounds: strong positive immunohistochemical reactions were found as a rule earlier than in human skin wounds. The detection of an increased expression of ICAM-1, VCAM-1 and P- and E-selectins can improve the wound age assessment in injuries with short survival times.
Dressler
J
J
Department of Legal Medicine, Technical University Medical School, Fetscherstrasse 74, D-01307, Dresden, Germany.
Bachmann
L
L
Strejc
P
P
Koch
R
R
Müller
E
E
D000818:Animals; D001344:Autopsy / Q000379:methods*; D004195:Disease Models, Animal; D019040:E-Selectin / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis*; D015603:Keratinocytes / Q000737:chemistry; D019041:L-Selectin / Q000032:analysis*; D051379:Mice; D019007:P-Selectin / Q000032:analysis*; D011180:Postmortem Changes; D013997:Time Factors; D019010:Vascular Cell Adhesion Molecule-1 / Q000032:analysis*; D014945:Wound Healing; D014947:Wounds and Injuries / Q000473:pathology*
D019040:E-Selectin; D019007:P-Selectin; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D019041:L-Selectin