"Malgun" (clear) cell change of gastric epithelium in chronic Helicobacter pylori gastritis.
To characterize the Helicobacter pylori gastritis-associated epithelial change, we analyzed 251 randomly selected gastric biopsies. The "malgun" (clear) cell change of the gastric epithelium was noted in 229 biopsies (91.2%). Malgun cells were characterized by large, pale nuclei with a euchromatin pattern, enlarged nucleoli, and clear cytoplasm. In the proliferative zone, individual malgun cells and small clusters were often in close contact with infiltrating neutrophils, suggesting that they had developed individually in the background of acute foveolitis. Mitotic figures of malgun cells were not infrequent, including atypical ones. In the surface epithelium, most malgun cells were in clusters that were often large enough to occupy wide epithelial segments. With Warthin-Starry triple staining, they were distinguished by the absence of silver impregnation, while other cells showed staining of the heterochromatin. They displayed prominent immunostaining for low molecular weight cytokeratin (No. 8). Most malgun cells were PCNA-positive in both surface and proliferative zones, whereas Ki67-positive cells were found only in the proliferative zone. It was suggested that a population of malgun cells, which were positive for PCNA only, were in the process of active DNA repair. The malgun cell change may represent a "cellular pattern of activation" in a population which had significant DNA damage, but somehow escaped the detection by the apoptosis system. The notion of "damage at the genetic level" was supported by the observation that these cells remained at least for 8 weeks after eradication of the H. pylori infection.
Lee
H
H
Department of Pathology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
Jang
J
J
Kim
Y
Y
Ahn
S
S
Gong
M
M
Choi
E
E
Lee
I
I
D000328:Adult; D000368:Aged; D002455:Cell Division; D002908:Chronic Disease; D005753:Gastric Mucosa / Q000473:pathology*; D005756:Gastritis / Q000382:microbiology* / Q000473:pathology* / Q000628:therapy; D016481:Helicobacter Infections; D016480:Helicobacter pylori; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008854:Microscopy, Electron; D008875:Middle Aged; D013194:Staining and Labeling