Suspended bed chromatography, a new approach in downstream processing.
A new technique in downstream processing, suspended bed chromatography has been developed. This hybrid technique exploiting the benefits of batch adsorption and the process advantages of an enclosed column system can be carried out using established contactors and adsorbents. A 44 cm I.D. IsoPak column and the anion-exchange cellulose Express-Ion Exchanger Q were used in the purification of ovalbumin from hen-egg white. After suspension of 16.25 kg Express-Ion Q in 500 l of feedstock containing 5 g protein/l, adsorption was effected by recirculation of the suspension using the IsoPak slurry preparation station. Protein-loaded adsorbent was collected in the IsoPak column unit, where it was washed and protein desorbed using gradient elution at a flow-rate of 300 cm/h. The entire process was complete in under 3 h. With the introduction of pump-packed column systems and the availability of mechanically strong adsorbents suitable for column separations, suspended bed chromatography offers a new approach to downstream processing and provides a less challenging alternative to batch separations.
Levison
P R
PR
Whatman International Ltd., Springfield Mill, Maidstone, Kent, UK. peter1@whatman.co.uk
Hopkins
A K
AK
Hathi
P
P
Badger
S E
SE
Mann
F
F
Dickson
N
N
Purdom
G
G
D000327:Adsorption; D002853:Chromatography, Liquid / Q000379:methods*; D010047:Ovalbumin / Q000032:analysis* / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D010047:Ovalbumin