title: Mechanical properties of bovine aortic endothelial cells in suspension studied by ultrasonic interferometry.
abstract: OBJECTIVE
  Cell adhesion phenomenon has been extensively studied in the last decade and was shown to be mediated by specialized molecules and driven by physical forces. Cohesion of the vessel wall cells is also dependent on adhesion molecules but less is known about the physical forces involved. To investigate endothelial cell/endothelial cell interaction from a mechanical point of view, we have used an ultrasonic interferometry device, named EchoCell, which has been previously designed to study red blood cell-red bood cell (RBC-RBC) interaction.
  
  
  METHODS
  Bovine aortic endothelial (BAE) cells were cultured, detached, then suspended in buffer and their mechanical and geometrical properties studied with the EchoCell system. The ultrasonic apparatus measures both the accumulation rate of cells in suspension on a solid plate and the acoustical impedances of the suspension and the sediment.
  
  
  RESULTS
  In suspension, BAE exhibited, in our experimental conditions (3x10(6) cells per ml), a spherical size evaluated by calculation at a mean radius of 7+/-2 microm. Moreover, no BAE aggregation occurred at the concentrations used. The acoustical impedance of the BAE suspensions calculated from all the samples studied, in the cell concentration range from 1.5x10(6) to 6x10(6) cells per ml, was 1.52x10(6) Rayl (kg m(-2) s(-1)). Furthermore, the acoustical impedance of the cell sediment was found to be independent on the initial cell suspension concentration and equal to 1.63x10(6) Rayl (kg m(-2) s(-1)). Estimation of the volume fraction of BAE inside the sediment allows to evaluate the ultrasonic velocity and the elastic bulk modulus of cells.
  
  
  CONCLUSIONS
  The ultrasonic interferometry method appears particularly interesting to study geometrical and mechanical (acoustical impedance, sound velocity, elastic bulk modulus) properties of BAE cells.
authors.affiliation: Groupe de Recherche en Physique et Biophysique, UFR Biomédicale, Université Paris V, 45 rue des Saintes-Pères, 75006, Paris, France. boynard@biomedicale.univ-paris5.fr
authors.forename: M
authors.initials: M
authors.lastname: Boynard
authors.forename: M P
authors.initials: MP
authors.lastname: Wautier
authors.forename: P
authors.initials: P
authors.lastname: Perrotin
authors.forename: J L
authors.initials: JL
authors.lastname: Wautier
mesh_terms: D000818:Animals; D001011:Aorta / Q000166:cytology; D002417:Cattle; D002448:Cell Adhesion / Q000502:physiology*; D002465:Cell Movement; D048429:Cell Size; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000000981:diagnostic imaging / Q000502:physiology*; D007368:Interferometry / Q000379:methods*; D015203:Reproducibility of Results; D014463:Ultrasonography