Serum-induced hypha formation in the dimorphic yeast Yarrowia lipolytica.
The dimorphic yeast Yarrowia lipolytica forms true hyphae in a medium containing N-acetylglucosamine. We made a new finding that serum is a very effective inducer of hypha formation of Y. lipolytica: serum induced its hyphal growth very quickly compared to N-acetylglucosamine (4 h vs. 10 h). Osmotic and oxidative stresses (0.2 M NaCl and 20 mM H2O2) inhibited the hypha formation induced by N-acetylglucosamine, but did not suppress the hypha formation triggered by serum. Serum-specific morphological mutants, which formed hyphae in the N-acetylglucosamine medium but not in serum medium, could be isolated. These results suggest that the signal triggered by serum may be transduced through a different pathway, at least in part, from that used for the N-acetylglucosamine signal in Y. lipolytica.
Kim
J
J
Department of Microbiology, Chungnam National University, Taejon, South Korea.
Cheon
S A
SA
Park
S
S
Song
Y
Y
Kim
J Y
JY
D000117:Acetylglucosamine / Q000494:pharmacology; D001769:Blood; D003470:Culture Media / Q000737:chemistry; D009997:Osmotic Pressure; D018384:Oxidative Stress; D004718:Saccharomycetales / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D015003:Yeasts / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*
D003470:Culture Media; D000117:Acetylglucosamine