Enantiomeric excess of a cruciferous phytoalexin, spirobrassinin, and its enantiomeric enrichment in an achiral HPLC system.
Enantiomeric purity of a cruciferous phytoalexin, spirobrassinin (1), was determined by chiral HPLC analysis. The enantiomeric excesses of two natural spirobrassinin fractions separated by nonchiral chromatography were considerably different. A significant enantiomeric enrichment was observed during the nonchiral chromatographic separation of an artificial enantiomeric mixture of 1.
Monde
K
K
Institute for Chemical Reaction Science, Tohoku University, Katahira 2-1-1, Sendai 980-8577, Japan. kmonde@icrs.tohoku.ac.jp
Harada
N
N
Takasugi
M
M
Kutschy
P
P
Suchy
M
M
Dzurilla
M
M
D001937:Brassica / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D010936:Plant Extracts / Q000737:chemistry*; D012717:Sesquiterpenes; D013057:Spectrum Analysis; D013141:Spiro Compounds / Q000737:chemistry*; D013237:Stereoisomerism; D013729:Terpenes; D013844:Thiazoles / Q000737:chemistry*; D000094162:Phytoalexins
D010936:Plant Extracts; D012717:Sesquiterpenes; D013141:Spiro Compounds; D013729:Terpenes; D013844:Thiazoles; C417935:spirobrassinin; D000094162:Phytoalexins