Detection of Microsporum canis in the skin scrapings and hairs of dogs with dermatophytosis based on sequences of the chitin synthase 1 gene.
In the present study, to confirm Microsporum canis infection rapidly, we detected the chitin synthase gene 1 (CHS1) gene of M. canis in the hair and skin samples of four dogs with dermatophytosis. Amplification of the DNAs in the four samples with CHS1 primers yielded fragments of about 620-bp. Nucleotide sequence analysis of the CHS1 gene fragments from samples and a reference strain of M. canis gene showed more than 99% similarity. The method presented in this study can rapidly detect the DNA of M. canis in skin scrapings, and we anticipate that it will be a useful microbiological tool for the diagnosis of M. canis infections in animals and humans.
Kano
R
R
Department of Pathobiology, Nihon University School of Veterinary Medicine, Fujisawa, Kanagawa, Japan. kano@brs.nihon-u.ac.jp
Nakamura
Y
Y
Watanabe
S
S
Hasegawa
A
A
D000818:Animals; D001483:Base Sequence; D002687:Chitin Synthase / Q000032:analysis* / Q000235:genetics; D003881:Dermatomycoses / Q000382:microbiology*; D004285:Dogs; D005800:Genes, Fungal; D006197:Hair / Q000382:microbiology; D008864:Microsporum / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D012867:Skin / Q000382:microbiology
D002687:Chitin Synthase