Degradation of 2,4,6-trichlorophenol via chlorohydroxyquinol in Ralstonia eutropha JMP134 and JMP222.
The aim of this work was to study the catabolic pathway of the pollutant 2,4,6-trichlorophenol in Ralstonia eutropha JMP134. 2,6-dichlorohydroquinone was detected as transient intermediate. Enzymatic transformations of 6-chlorohydroxyquinol to 2-chloromaleylacetate, and of this compound to maleylacetate were detected in crude extracts. Therefore, the degradation of 2,4,6-trichlorophenol proceeded through an hydroxyquinol pathway, different from the other chloroaromatic pathways reported in this strain. The same results were observed in two other 2,4,6-trichlorophenol degrading strains: R. eutropha JMP222, a derivative of strain JMP134 lacking the chlorocatechol catabolism-encoding pJP4 plasmid, and a river isolate, Ralstonia sp. PZK.
Padilla
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L
Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Alameda, Santiago, Chile.
Matus
V
V
Zenteno
P
P
González
B
B
D001673:Biodegradation, Environmental; D002733:Chlorophenols / Q000378:metabolism*; D002849:Chromatography, Gas; D020118:Cupriavidus necator / Q000201:enzymology* / Q000254:growth & development; D049308:Dioxygenases; D006873:Hydroquinones / Q000378:metabolism*; D010105:Oxygenases / Q000378:metabolism*
C091167:6-chlorohydroxyquinol; D002733:Chlorophenols; D006873:Hydroquinones; D010105:Oxygenases; C091168:6-chlorohydroxyquinol 1,2-dioxygenase; D049308:Dioxygenases; C024564:2,4,6-trichlorophenol