Developmental profile of kainate receptor subunit KA1 revealed by Cre expression in YAC transgenic mice.
To determine the spatio-temporal expression in brain of the high-affinity kainate receptor subunit KA1, we generated transgenic mice expressing Cre recombinase from the KA1 gene on a chromosomally integrated 550 kb yeast artificial chromosome (YAC). Activity of the KA1 gene promoter during brain development was visualized by Cre immunohistochemistry, and by X-gal staining of beta-galactosidase induced by Cre recombinase in double transgenic KA1-Cre/lacZ indicator mice. During early brain development, expression from the YAC-carried KA1-Cre transgene was observed in all major brain areas, predicting a function for KA1 in the developing central nervous system. In the adult brain, KA1-Cre transgene expression was restricted mainly to hippocampal CA3 pyramidal and dentate gyrus granule cells, an adult expression pattern characteristic for the endogenous KA1 alleles. KA1-Cre transgenic mice may help in elucidating the role of floxed genes ablated in vivo in KA1 expressing neurons.
Kask
K
K
Department of Molecular Neuroscience, Max-Planck-Institute for Medical Research, Jahnstrasse 29, 69120, Heidelberg, Germany.
Jerecic
J
J
Zamanillo
D
D
Wilbertz
J
J
Sprengel
R
R
Seeburg
P H
PH
D000375:Aging / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development / Q000378:metabolism*; D017930:Genes, Reporter / Q000502:physiology; D007150:Immunohistochemistry; D019426:Integrases / Q000235:genetics / Q000378:metabolism*; D007763:Lac Operon / Q000502:physiology; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics; D020033:Protein Isoforms / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D018092:Kainic Acid Receptors / Q000235:genetics / Q000378:metabolism*; D014018:Tissue Distribution; D014764:Viral Proteins
D019426:Integrases; D020033:Protein Isoforms; D012333:RNA, Messenger; D018092:Kainic Acid Receptors; D014764:Viral Proteins; C045073:Cre recombinase