Identifying the promoter region of the human brain sodium channel subtype II gene (SCN2A).
Sodium channel genes are highly regulated. To begin analyzing the human brain sodium channel subtype II gene, SCN2A, at the transcriptional level, we mapped multiple transcriptional start sites within a 397 bp stretch of the 5'-UTR and -flanking region. When inserted into a basic luciferase reporter vector, this 397 bp region can promote luciferase expression in transiently transfected neuroblastoma cells, but not in non-neuronal cells. Thus, this study provides the initial description of a functional promoter in a human voltage-gated sodium channel gene.
Schade
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Department of Psychiatry and Behavioral Neurobiology, Sparks Center, room 1075, University of Alabama at Birmingham, Birmingham, AL 35294-0017, USA. schade@uab.edu
Brown
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D020121:5' Untranslated Regions / Q000235:genetics; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D017930:Genes, Reporter; D006801:Humans; D008156:Luciferases / Q000235:genetics; D008969:Molecular Sequence Data; D062551:NAV1.2 Voltage-Gated Sodium Channel; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009447:Neuroblastoma; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D015222:Sodium Channels / Q000235:genetics*; D016385:TATA Box; D014158:Transcription, Genetic; D014162:Transfection; D014407:Tumor Cells, Cultured
D020121:5' Untranslated Regions; D062551:NAV1.2 Voltage-Gated Sodium Channel; D009419:Nerve Tissue Proteins; D011993:Recombinant Fusion Proteins; C568251:SCN2A protein, human; D015222:Sodium Channels; D008156:Luciferases