title: Heart mitochondrial DNA and enzyme changes during early human development.
abstract: Previous studies in our laboratory demonstrated significant changes in bovine heart mitochondrial bioenergetics during fetal growth and development. To further understand mitochondrial biogenesis in early human development, the activity and subunit content levels of specific mitochondrial enzymes in fetal and neonatal heart were determined. Comparing early gestation (EG, 45-65 day) later gestation (LG, 85-110 day) and neonate (birth-1 month), specific activity of citrate synthase (CS), a Krebs cycle enzyme showed a 2 fold increase from EG to LG and a 2 fold increase from LG to neonate. Specific activities of complex IV and complex V increased similarly 1.8-2 fold from EG to LG. However during the later fetal period from LG to neonate, complex IV activity increased only 1.3 fold and complex V showed no significant increase. Peptide content of COX-II subunit increased 2 fold from EG to LG and by 3.5 fold from LG to neonate. Levels of COX-IV and ATP synthase alpha subunits were undetectable in EG hearts, clearly detectable in LG heart and 3 fold increased from LG to neonate. Unexpectedly, mitochondrial transcription factor A (mt-TFA) levels were not significantly different during these developmental stages. Mitochondrial DNA (mtDNA) levels increased 1.8 fold from EG to LG, and 3.8 fold increase from EG to neonate and correlated with CS activity levels. In conclusion, these data indicate coordinated regulation of some nuclear-encoded (COX-IV and CS activity) and mitochondrial components (COX-II and mtDNA), and strongly suggest that mitochondrial content increases particularly during the early fetal cardiac development and reveal a distinct pattern of regulation for mt-TFA.
authors.affiliation: The Molecular Cardiology Institute, Highland Park, New Jersey 08904, USA.
authors.forename: J
authors.initials: J
authors.lastname: Marin-Garcia
authors.forename: R
authors.initials: R
authors.lastname: Ananthakrishnan
authors.forename: M J
authors.initials: MJ
authors.lastname: Goldenthal
mesh_terms: D000704:Analysis of Variance; D015153:Blotting, Western; D002950:Citrate (si)-Synthase / Q000378:metabolism; D004272:DNA, Mitochondrial / Q000032:analysis; D005314:Embryonic and Fetal Development; D005318:Fetal Heart / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D006321:Heart / Q000196:embryology*; D006352:Heart Ventricles; D006801:Humans; D007231:Infant, Newborn; D008929:Mitochondria, Heart / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D025261:Mitochondrial Proton-Translocating ATPases; D006180:Proton-Translocating ATPases / Q000378:metabolism
chemical_list: D004272:DNA, Mitochondrial; C021983:complex V (mitochondrial oxidative phosphorylation system); D002950:Citrate (si)-Synthase; D025261:Mitochondrial Proton-Translocating ATPases; D006180:Proton-Translocating ATPases