Mutational analysis of the multicopy hao gene coding for hydroxylamine oxidoreductase in Nitrosomonas sp. strain ENI-11.
The ammonia-oxidizing bacterium Nitrosomonas sp. strain ENI-11 contains three copies of the hao gene (hao1, hao2, and hao3) coding for hydroxylamine oxidoreductase (HAO). Three single mutants (hao1::kan, hao2::kan, or hao3::kan) had 68 to 75% of the wild-type growth rate and 58 to 89% of the wild-type HAO activity when grown under the same conditions. A double mutant (hao1::kan and hao3::amp) also had 68% of the wild-type growth and 37% of the wild-type HAO activity.
Yamagata
A
A
Department of Fermentation Technology, Hiroshima University, Higashi-Hiroshima, Japan.
Hirota
R
R
Kato
J
J
Kuroda
A
A
Ikeda
T
T
Takiguchi
N
N
Ohtake
H
H
D004252:DNA Mutational Analysis; D004269:DNA, Bacterial / Q000737:chemistry; D009606:Nitrosomonas / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D010088:Oxidoreductases / Q000235:genetics*; D016133:Polymerase Chain Reaction
D004269:DNA, Bacterial; D010088:Oxidoreductases; C021401:hydroxylamine dehydrogenase