Dynamic interfacial properties of human apolipoproteins A-IV and B-17 at the air/water and oil/water interface.
Viscoelastic behavior of proteins at interfaces is a critical determinant of their ability to stabilize emulsions. We therefore used air bubble surfactometry and drop volume tensiometry to examine the dynamic interfacial properties of two plasma apolipoproteins involved in chylomicron assembly: apolipoprotein A-IV and apolipoprotein B-17, a recombinant, truncated apolipoprotein B. At the air/water interface apolipoproteins A-IV and B-17 displayed wide area - tension loops with positive phase angles indicative of viscoelastic behavior, and suggesting that they undergo rate-dependent changes in surface conformation in response to changes in interfacial area. At the triolein/water interface apolipoprotein A-IV displayed maximal surface activity only at long interface ages, with an adsorption rate constant of 1.0 3 10(-)(3) sec(-)(1), whereas apolipoprotein B-17 lowered interfacial tension even at the shortest interface ages, with an adsorption rate constant of 9.3 3 10(-)(3) sec(-)(1). Apolipoprotein A-IV displayed an expanded conformation at the air/water interface and a biphasic compression isotherm, suggesting that its hydrophilic amphipathic helices move in and out of the interface in response to changes in surface pressure. We conclude that apolipoproteins A-IV and B-17 display a combination of interfacial activity and elasticity particularly suited to stabilizing the surface of expanding triglyceride-rich particles.
Weinberg
R B
RB
Section of Gastroenterology, Department of Pathology, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA.
Cook
V R
VR
DeLozier
J A
JA
Shelness
G S
GS
D000388:Air; D016632:Apolipoprotein A-I / Q000737:chemistry; D001054:Apolipoproteins A / Q000737:chemistry*; D001055:Apolipoproteins B / Q000737:chemistry*; D004548:Elasticity; D006801:Humans; D009821:Oils; D011312:Pressure; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry; D013499:Surface Properties; D013500:Surface Tension; D014783:Viscosity; D014867:Water
D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D001055:Apolipoproteins B; D009821:Oils; D011994:Recombinant Proteins; C022205:apolipoprotein A-IV; C416027:apolipoprotein b-17; D014867:Water