The Synechococcus strain PCC 7942 glnN product (glutamine synthetase III) helps recovery from prolonged nitrogen chlorosis.
We report the cloning and sequencing of the glnN gene encoding a class III glutamine synthetase from the cyanobacterium Synechococcus strain PCC 7942. Mapping of the transcriptional start site revealed a DNA sequence in the promoter region that resembles an imperfect NtcA binding motif. Expression of glnN is impaired in NtcA- and P(II)-deficient mutants. The only parameter which was negatively affected in the glnN mutant compared to the wild type was the recovery rate of prolonged nitrogen-starved cells with low concentrations of combined nitrogen.
Sauer
J
J
Institut für Mikrobiologie und Molekularbiologie der Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
Dirmeier
U
U
Forchhammer
K
K
D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D000458:Cyanobacteria / Q000201:enzymology* / Q000235:genetics; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005974:Glutamate-Ammonia Ligase / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009584:Nitrogen / Q000378:metabolism*; D051922:PII Nitrogen Regulatory Proteins; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D051922:PII Nitrogen Regulatory Proteins; D014157:Transcription Factors; C075617:ntcA protein, Synechococcus; C404143:PIID regulatory protein, Bacteria; D005974:Glutamate-Ammonia Ligase; D009584:Nitrogen