Effect of the aminosteroid U73122 on prostaglandin E(2) production in a murine clonal osteoblast-like cell line, MC3T3-E1.
Prostaglandin E(2) production stimulated by various agents (arachidonic acid, prostaglandin F(2alpha), ionomycin, the calcium ionophore A23187, and melittin) was investigated after pretreatment of murine osteoblast-like MC3T3-E1 cells with the putative phospholipase C blocker, U73122. The aminosteroid dose dependently inhibited prostaglandin E(2) production induced by all agonists, except arachidonic acid. The results suggest an inhibitory role of U73122 on phospholipase A(2) activity or activation.
Leis
H J
HJ
University Children's Hospital, Department of Biochemical Analysis and Mass Spectrometry, Auenbruggerplatz 30, A-8036, Graz, Austria. hans.leis@kfunigraz.ac.at
Köfeler
H
H
Raspotnig
G
G
Zach
D
D
Fauler
G
G
Windischhofer
W
W
D000019:Abortifacient Agents / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D016718:Arachidonic Acid / Q000378:metabolism; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002250:Carbon Radioisotopes; D002460:Cell Line; D002999:Clone Cells; D015237:Dinoprost / Q000494:pharmacology; D015232:Dinoprostone / Q000096:biosynthesis*; D004789:Enzyme Activation; D004963:Estrenes / Q000494:pharmacology*; D015759:Ionomycin / Q000494:pharmacology; D008555:Melitten / Q000494:pharmacology; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D010741:Phospholipases A / Q000378:metabolism; D011760:Pyrrolidinones / Q000494:pharmacology*
D000019:Abortifacient Agents; D000900:Anti-Bacterial Agents; D002250:Carbon Radioisotopes; D004963:Estrenes; D010726:Phosphodiesterase Inhibitors; D011760:Pyrrolidinones; C060229:1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione; D008555:Melitten; D016718:Arachidonic Acid; D000001:Calcimycin; D015759:Ionomycin; D015237:Dinoprost; D010741:Phospholipases A; D015232:Dinoprostone; D002118:Calcium