title: Bcl-2 expression in sequential biopsies of potentially malignant oral mucosal lesions assessed by immunocytochemistry.
abstract: OBJECTIVE
  To examine, for the first time Bcl-2 expression in sequential (autogenous) oral mucosal biopsies taken from the same sites in a gender, risk-factor matched, Caucasoid sample, over a 21-year period.
  
  
  METHODS
  Retrospective immunocytochemical longitudinal study of archival serial biopsies.
  
  
  METHODS
  Computer records were used to identify biopsy specimens derived from 12 patients. These were divided into four groups: (1) Histologically innocuous lesions which remained histologically innocuous. (2) Dysplastic lesions which remained dysplastic. (3) Histologically innocuous lesions which later progressed to squamous cell carcinoma (SCC). (4) Dysplastic lesions which later progressed to SCC. This represented 65 biopsies in total. Bcl-2 expression was studied using mouse antihuman BCL-2 oncoprotein clone 124 (Dako, Denmark).
  
  
  RESULTS
  Generally, there was a lack of Bcl-2 immunoreactivity in the epithelium, with one exception in dysplastic epithelium from a group (3) patient.
  
  
  CONCLUSIONS
  These findings suggest that in our series, Bcl-2 is not expressed early in oral premalignant lesions and appears to contradict previous reports. Possible explanations for this disparity are considered.
authors.affiliation: School of Clinical Dentistry, The Queen's University of Belfast, Royal Group of Hospitals, Grosvenor Rd, Belfast BT12 6BP.
authors.forename: R L
authors.initials: RL
authors.lastname: McAlinden
authors.forename: P
authors.initials: P
authors.lastname: Maxwell
authors.forename: S
authors.initials: S
authors.lastname: Napier
authors.forename: P
authors.initials: P
authors.lastname: Hamilton
authors.forename: C G
authors.initials: CG
authors.lastname: Cowan
authors.forename: F T
authors.initials: FT
authors.lastname: Lundy
authors.forename: P J
authors.initials: PJ
authors.lastname: Lamey
authors.forename: J J
authors.initials: JJ
authors.lastname: Marley
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D001706:Biopsy; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D016022:Case-Control Studies; D002471:Cell Transformation, Neoplastic / Q000473:pathology; D004396:Coloring Agents; D015986:Confounding Factors, Epidemiologic; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000473:pathology*; D011230:Precancerous Conditions / Q000473:pathology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis*; D012307:Risk Factors; D012907:Smoking
chemical_list: D004396:Coloring Agents; D019253:Proto-Oncogene Proteins c-bcl-2