title: Transient isotachophoresis for sensitivity enhancement in capillary electrophoresis-mass spectrometry for peptide analysis.
abstract: Transient isotachophoresic (ITP) focusing was used for the on-line analysis of peptides by capillary zone electrophoresis-mass spectrometry (CZE-MS), allowing injection volumes of up to 0.9 microL. A sheath liquid electrospray interface was used with a single quadrupole mass analyzer. First, the technique was applied to the qualitative analysis of a tryptic digest of cytochrome c, resulting in low-background, high-quality spectra. Second, the linear range was investigated by selected ion monitoring (SIM) for a peptidomimetic direct thrombin inhibitor melagatran (Mr 429.5) and two endogenous peptides, substance P (Mr 1348) and calcitonin gene-related peptide (alpha-CGRP; Mr 3806).
authors.affiliation: DMPK & Bioanalytical Chemistry, AstraZeneca R&D Mölndal, Sweden. marita.s.larsson@astrazeneca.com
authors.forename: M
authors.initials: M
authors.lastname: Larsson
authors.forename: E S
authors.initials: ES
authors.lastname: Lutz
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D010455:Peptides / Q000032:analysis*; D012680:Sensitivity and Specificity
chemical_list: D010455:Peptides