title: A monoclonal antibody to the DEC-205 endocytosis receptor on human dendritic cells.
abstract: DEC-205 is a multilectin receptor for adsorptive endocytosis, expressed in mouse dendritic cells (DC) and some epithelia. DEC-205 is homologous to the macrophage mannose receptor (MMR). A cDNA for murine DEC-205 was used to identify 3 overlapping human DEC-205 clones from a lymphocyte library. The human homologue is a transmembrane protein of 1722 aminoacids with 10 externally disposed C-type lectin domains having 77% identity to the mouse counterpart. The NH(2) terminal cysteine-rich and fibronectin type II domains were expressed and used to immunize mice. A hybridoma, MG38, which specifically recognized the immunogen was obtained from a DEC-205 knockout mouse. The antibody precipitated a 205 kD protein from metabolically labeled, monocyte-derived DCs. MG38 labeled mature monocyte-derived DCs but showed weak or no labeling of other peripheral blood mononuclear cells. In tissue sections, MG38 identified DEC-205 on thymic cortical epithelium and DCs in the thymic medulla and tonsillar T cell areas. In contrast, an anti-MMR antibody stained DEC-205 negative, macrophages in the thymus cortex, the trabeculae of the thymus and tonsil, as well as efferent lymphatics in the tonsil. Therefore, the MG38 anti-DEC-205 antibody is useful for identifying DCs and reveals clear differences in sites where MMR and DEC-205 are expressed in lymphoid tissues.
authors.affiliation: Laboratory of Molecular Immunology, Rockefeller University, New York, New York, USA.
authors.forename: M
authors.initials: M
authors.lastname: Guo
authors.forename: S
authors.initials: S
authors.lastname: Gong
authors.forename: S
authors.initials: S
authors.lastname: Maric
authors.forename: Z
authors.initials: Z
authors.lastname: Misulovin
authors.forename: M
authors.initials: M
authors.lastname: Pack
authors.forename: K
authors.initials: K
authors.lastname: Mahnke
authors.forename: M C
authors.initials: MC
authors.lastname: Nussenzweig
authors.forename: R M
authors.initials: RM
authors.lastname: Steinman
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015703:Antigens, CD; D002461:Cell Line, Transformed; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D004705:Endocytosis / Q000276:immunology*; D005434:Flow Cytometry; D015870:Gene Expression; D006801:Humans; D037181:Lectins, C-Type; D008070:Lipopolysaccharides / Q000276:immunology; D008221:Lymphoid Tissue; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D018345:Mice, Knockout; D015778:Minor Histocompatibility Antigens; D009000:Monocytes / Q000166:cytology / Q000276:immunology; D011956:Receptors, Cell Surface / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; C093569:DEC-205 receptor; D037181:Lectins, C-Type; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D015778:Minor Histocompatibility Antigens; D011956:Receptors, Cell Surface