title: Capillary electrophoretic analysis of wild type and mutant Proteus penneri outer membrane proteins.
abstract: In this study the virulence factors, outer membrane proteins (OMP), lipopolysaccharides (LPS), hemolysin, and the in vivo and in vitro virulence of wild-type Proteus penneri 357 and its two isogenic mutant variants--a transposon and a spontaneous mutant--were examined. The OMPs of these variants were analyzed by a new and fast technique, "dynamic sieving" capillary electrophoresis (CE). The OMP profiles were dominated by two peaks (39 and 43 kDa). In the P. penneri clone examined, both the transposon and the spontaneous mutations induced significant changes in the OMP patterns (in the relative percentage of the dominant proteins). CE was suitable for the comparative analysis of bacterial protein patterns in the genetic variants of this strain, and provided valuable results in connection with the bacteriological virulence. The LPS composition of the genetic variants also showed alterations. The wild type of P. penneri 357 showed a typical ladder pattern, an "S" form, and the mutants possessed "R" LPS patterns (only few bands) in the gels. In the bacteriological virulence tests the wild type of P. penneri 357 was virulent in the in vivo, and toxic in the in vitro assays, while both mutants showed neither toxicity nor pathogenicity.
authors.affiliation: Department of Medical Microbiology and Immunology, Faculty of Medicine, University of Pécs, Hungary. ikustos@apacs.pote.hu
authors.forename: I
authors.initials: I
authors.lastname: Kustos
authors.forename: V
authors.initials: V
authors.lastname: Tóth
authors.forename: B
authors.initials: B
authors.lastname: Kocsis
authors.forename: I
authors.initials: I
authors.lastname: Kerepesi
authors.forename: L
authors.initials: L
authors.lastname: Emody
authors.forename: F
authors.initials: F
authors.lastname: Kilár
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000032:analysis* / Q000235:genetics; D019075:Electrophoresis, Capillary / Q000379:methods; D009154:Mutation; D011511:Proteus
chemical_list: D001425:Bacterial Outer Membrane Proteins