High-throughput screening of enzyme libraries.
Directed evolution is becoming a widely used technique for modifying or enhancing protein performance. Ultimately, the success of directed protein evolution experiments hinges on the efficiency of the methods used to screen libraries for mutants with properties of interest. Although there is still a paucity of general methods for enzyme library screening, in recent years a number of promising strategies have emerged and are increasingly being used to explore challenging issues in protein engineering.
Olsen
M
M
Department of Chemistry and Biochemistry, University of Texas at Austin, 78712, USA.
Iverson
B
B
Georgiou
G
G
D019020:Directed Molecular Evolution; D004798:Enzymes; D015723:Gene Library; D019151:Peptide Library; D015202:Protein Engineering / Q000379:methods*
D004798:Enzymes; D019151:Peptide Library