Modulation of collagen-induced arthritis by IL-4 and dexamethasone: the synergistic effect of IL-4 and dexamethasone on the resolution of CIA.
We investigated effects of IL-4, dexamethasone (DXM), and the combination of IL-4 and DXM, low- or high-dose, on collagen-induced arthritis (CIA) in DBA/1 mice and correlated severity of arthritis with changes in IL-10 and IFN-gamma. Compared with control mice, mice treated with IL-4 had increased IL-10 with the same degree of arthritis, whereas mice treated with high-dose DXM had decreased IL-10 and increased IFN-gamma production with less severe arthritis. Mice treated with low-dose DXM showed the absence of IL-10 and increased IFN-gamma production with a trend toward the resolution of arthritis. Mice treated with IL-4 and low-dose DXM had neither IL-10 nor IFN-gamma production but revealed less severe arthritis, compared with mice treated with low-dose DXM alone. These results suggest that the beneficial effects of high-dose DXM and the combination of IL-4 and DXM on CIA are independent of IL-10 and IFN-gamma.
Kang
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I
Department of Internal Medicine, College of Medicine, Hallym University, South Korea. insoo.kang@yale.edu
Lee
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WW
Lee
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Y
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D001169:Arthritis, Experimental / Q000276:immunology* / Q000517:prevention & control*; D002417:Cattle; D003094:Collagen / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis; D003907:Dexamethasone / Q000008:administration & dosage / Q000494:pharmacology*; D004306:Dose-Response Relationship, Immunologic; D004338:Drug Combinations; D004357:Drug Synergism; D007271:Injections, Intradermal; D007274:Injections, Intraperitoneal; D015847:Interleukin-4 / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D012720:Severity of Illness Index
D000276:Adjuvants, Immunologic; D016207:Cytokines; D004338:Drug Combinations; D015847:Interleukin-4; D003907:Dexamethasone; D003094:Collagen