title: HLA-B27 expression by flow cytometry: an analysis of 7 years quality assurance data.
abstract: The Royal College of Pathologists of Australasia Quality Assurance Programs Pty. Ltd. has been monitoring HLA-B27 assignment by flow cytometry for 7 years as part of the Immunology Program. Here we present data that demonstrates a gradual improvement in reports of false positive and negative results. Many participating laboratories demonstrate an ability to assign HLA-B27 status correctly by flow cytometric means. This ability appears to be independent of reagent and methodology. However a small number of laboratories produce consistently unacceptable results that suggest poor quality assurance practice.
authors.affiliation: Department of Immunology, Allergy and Arthritis, Flinders Medical Centre, Adelaide SA5047, Bedford Park, Australia. peter.macardle@flinders.edu.au
authors.forename: P J
authors.initials: PJ
authors.lastname: Macardle
authors.forename: R
authors.initials: R
authors.lastname: McEvoy
authors.forename: S
authors.initials: S
authors.lastname: Jovanovich
mesh_terms: D005434:Flow Cytometry / Q000379:methods*; D015796:HLA-B27 Antigen / Q000032:analysis*; D006801:Humans; D007753:Laboratories / Q000592:standards; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D011786:Quality Control; D015203:Reproducibility of Results
chemical_list: D015796:HLA-B27 Antigen