[Preparation of antibodies to roridin A and their applications].
Polyclonal rabbit antibodies against a conjugate synthesized through condensing BSA and disubstituted roridin A hemisuccinate allowed roridin A to be determined in solutions at a sensitivity of 0.2 ng/ml. The cross-reactivity of structural analogues--roridin A, verrucarin, and verrucarol--amounted to 100, 2.5, and 0.03%, respectively. The data showed that these antibodies determine roridin A in an indirect heterogeneous enzyme immunoassay in cereal straw samples at a sensitivity of 20 micrograms/kg.
Birkin
A A
AA
Russian Institute of Veterinary Sanitary, Hygiene, and Ecology, Russian Academy of Agricultural Sciences, Moscow, Russia.
Kononenko
G P
GP
Zorian
V G
VG
Soboleva
N A
NA
Zotova
E V
EV
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000917:Antibody Formation; D003429:Cross Reactions; D009183:Mycotoxins / Q000737:chemistry / Q000276:immunology*; D011817:Rabbits; D014255:Trichothecenes / Q000737:chemistry / Q000276:immunology
D000906:Antibodies; D009183:Mycotoxins; D014255:Trichothecenes; C038849:roridin A