Analysis of acyclovir by high performance capillary electrophoresis with on-column amperometric detection.
The separation of acyclovir (ACV) by high performance capillary electrophoresis (HPCE) with on-column amperometric detection using alpha-amino-5-mercapto-3,4-dithiazole (AMD) as internal standard is described. The calibration line was linear in the range of 0.5-20 mg/L of ACV. The detection limit was 0.15 mg/L of ACV. Its recovery ranged from 98 to 101% with relative standard deviations (RSDs) from 1.9 to 3.2% (n = 5). This method was successfully used for determining ACV in some pharmaceuticals and human urine. Comparable results with HPCE with ultraviolet (UV) detection and amperometric detection were obtained.
Zhang
S
S
Center of Instrumental Analysis and Chemistry Department, Zhengzhou University, P.R. China. zhangshusheng@371.net
Yuan
Z
Z
Liu
H
H
Zou
H
H
Xiong
H
H
Wu
Y
Y
D000212:Acyclovir / Q000032:analysis* / Q000302:isolation & purification*; D000998:Antiviral Agents / Q000032:analysis* / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods; D006801:Humans
D000998:Antiviral Agents; D000212:Acyclovir