Transgenic methods for increasing Drosophila life span.
At least five different transgenic approaches have been applied to the study of Drosophila aging. There are two single component systems: transgenes with native (normal) promoters and transgenes with heterologous promoters; as well as three binary systems: 'GAL4/UAS', 'FLP-out' and 'tet-on'. These approaches vary in ability to meet several technical challenges, and the relative advantages and disadvantages of each are discussed. Using these techniques, over-expression of the hsp70, Cu/ZnSOD and MnSOD genes has each been demonstrated to increase Drosophila life span.
Tower
J
J
Department of Biological Sciences, SHS172, University of Southern California, Los Angeles, CA 90089-1340, USA. jtower@usc.edu
D000375:Aging / Q000235:genetics; D000818:Animals; D030801:Animals, Genetically Modified; D004330:Drosophila / Q000235:genetics* / Q000502:physiology*; D005821:Genetic Techniques; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D008136:Longevity / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D013482:Superoxide Dismutase / Q000235:genetics
D018840:HSP70 Heat-Shock Proteins; D013482:Superoxide Dismutase