The use of 1H-NMR spectroscopy and refractometry for investigation of the distribution of nonelectrolytes of N-alcohol series between human red blood cells and extracellular medium.
Comparative analysis of 1H NMR spectroscopy and refractometry with respect to their application for investigating the distribution of nonelectrolytes of n-alcohol series (ethanol, 1,2-propanediol, glycerol) and polyethylene glycols (PEGs) with molecular masses of 400, 600, 1500 between human erythrocytes and extracellular medium was performed. The distribution coefficients (Q) for solutions of ethanol, 1,2-propanediol, glycerol, PEG-400, PEG-600 and PEG-1500 were obtained. The Q values decreased with the increase in the nonelectrolyte molecular mass from 1.23+/-0.12 for ethanol to 0.40+/-0.08 for PEG-1500 (1H NMR spectroscopy) and from 2.6+/-0.12 for ethanol to 0.23+/-0.03 for PEG-1500 (refractometry). It was shown that 1H-NMR high-resolution spectroscopy ensures more precise determination of Q values for nonelectrolytes with low molecular masses; for PEGs with high molecular masses, the accuracy of Q value calculation by this method was about 20%. On the contrary, refractometry can be used for investigating substances with high molecular masses; the error of Q value determination for solution of low-refractive substances, such as ethanol, may be more than 50%.
Kucherenko
Y U
YU
Institute for Problems of Cryobiology and Cryomedicine, National Academy of Sciences, Kharkov, Ukraine. cryo@online.kharkov.ua
Moiseev
V A
VA
D004573:Electrolytes; D004912:Erythrocytes / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005110:Extracellular Space; D005990:Glycerol / Q000378:metabolism*; D006801:Humans; D006859:Hydrogen; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D011092:Polyethylene Glycols / Q000378:metabolism*; D019946:Propylene Glycol / Q000378:metabolism*; D012031:Refractometry / Q000379:methods
D004573:Electrolytes; D000431:Ethanol; D011092:Polyethylene Glycols; D019946:Propylene Glycol; D006859:Hydrogen; D005990:Glycerol