Genomics of the 35-kb pvd locus and analysis of novel pvdIJK genes implicated in pyoverdine biosynthesis in Pseudomonas aeruginosa.
Novel putative pyoverdine synthetase pvdIJK genes were found upstream of pvdD in the 6.2-Mb chromosome of Pseudomonas aerugilosa strain PAO1. These genes formed a locus implicated in pyoverdine biosynthesis. Sequence analysis showed that the product of these genes shared 43%, 60% and 57% identity with PvdD. PvdIJK are thought to be implicated in synthesis of pyoverdine, a siderophore chelating Fe3+. A pvdI mutant was obtained by gene disruption mutagenesis and confirmed by Southern hybridization. The pvdl mutant produced gave no significant growth on solid media supplemented with the iron chelator 2,2-dipyridyl; while the PvdI- phenotype abolished pyoverdine fluorescence. The role of PvdI in pathogenicity was tested by measuring the in vivo growth of P. aeruginosa wild-type and mutant strains in a chronic lung infection rat model, and by measuring the competitive infectivity index into a neutropenic mice model. The data obtained confirmed the importance of PvdI in virulence and iron uptake.
Lehoux
D E
DE
Centre de recherché sur la fonction, structure et ingénierie des protéines, pavillon Charles-Eugène Marchand et Faculté de Médecine, Université Laval, Canada.
Sanschagrin
F
F
Levesque
R C
RC
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins; D016680:Genome, Bacterial; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009842:Oligopeptides; D009876:Operon / Q000235:genetics*; D010453:Peptide Synthases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010860:Pigments, Biological / Q000096:biosynthesis* / Q000235:genetics; D011552:Pseudomonas Infections / Q000382:microbiology / Q000503:physiopathology; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism / Q000472:pathogenicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D009842:Oligopeptides; D010860:Pigments, Biological; C042453:pyoverdin; D010453:Peptide Synthases; C091136:pyoverdine synthetase protein, Pseudomonas