title: Intrinsic susceptibility of rhesus macaque peripheral CD4(+) T cells to simian immunodeficiency virus in vitro is predictive of in vivo viral replication.
abstract: Previous studies with simian immunodeficiency virus (SIV) infection of rhesus macaques suggested that the intrinsic susceptibility of peripheral blood mononuclear cells (PBMC) to infection with SIV in vitro was predictive of relative viremia after SIV challenge. The present study was conducted to evaluate this parameter in a well-characterized cohort of six rhesus macaques selected for marked differences in susceptibility to SIV infection in vitro. Rank order relative susceptibility of PBMC to SIVsmE543-3-infection in vitro was maintained over a 1-year period of evaluation. Differential susceptibility of different donors was maintained in CD8(+) T-cell-depleted PBMC, macrophages, and CD4(+) T-cell lines derived by transformation of PBMC with herpesvirus saimiri, suggesting that this phenomenon is an intrinsic property of CD4(+) target cells. Following intravenous infection of these macaques with SIVsmE543-3, we observed a wide range in plasma viremia which followed the same rank order as the relative susceptibility established by in vitro studies. A significant correlation was observed between plasma viremia at 2 and 8 weeks postinoculation and in vitro susceptibility (P < 0.05). The observation that the two most susceptible macaques were seropositive for simian T-lymphotropic virus type 1 may suggests a role for this viral infection in enhancing susceptibility to SIV infection in vitro and in vivo. In summary, intrinsic susceptibility of CD4(+) target cells appears to be an important factor influencing early virus replication patterns in vivo that should be considered in the design and interpretation of vaccine studies using the SIV/macaque model.
authors.affiliation: Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland 20853, USA.
authors.forename: S
authors.initials: S
authors.lastname: Goldstein
authors.forename: C R
authors.initials: CR
authors.lastname: Brown
authors.forename: H
authors.initials: H
authors.lastname: Dehghani
authors.forename: J D
authors.initials: JD
authors.lastname: Lifson
authors.forename: V M
authors.initials: VM
authors.lastname: Hirsch
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000821:virology*; D002460:Cell Line; D004198:Disease Susceptibility; D008253:Macaca mulatta; D019713:Receptors, CCR5 / Q000032:analysis; D016097:Simian Acquired Immunodeficiency Syndrome / Q000209:etiology*; D015302:Simian Immunodeficiency Virus / Q000502:physiology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014766:Viremia / Q000209:etiology; D014779:Virus Replication
chemical_list: D019713:Receptors, CCR5