Fetal striatal transplants reinstate the electrophysiological response of pallidal neurons to systemic apomorphine challenge in rats with excitotoxic striatal lesions.
Previous studies with single-unit recording and 2-[14C]deoxyglucose quantitative autoradiography have shown that systemic administration of apomorphine increases the functional activity of pallidal neurons, and that the enhancement in the globus pallidus (GP) activity is abolished by striatal lesions. The present study employing electrophysiological techniques tested whether embryonic striatal tissue implanted in the excitotoxically damaged striatum of rats may affect the lesion-induced alteration in the neuronal response of GP to apomorphine. Systemically administered apomorphine significantly increased spontaneously firing rates of GP cells. The blockade of dopamine receptors with haloperidol reversed the increased rate to baseline levels. Quinolinate-induced striatal lesions attenuated the rate-increasing effect of apomorphine. Embryonic striatal grafts placed in the lesioned striatum restored the response of GP cells to systemic apomorphine. The graft-mediated restoration of the GP neuron response to apomorphine were accompanied by an improvement in the motor asymmetry induced by this drug. Considering previous anatomical data to demonstrate extensive innervation of the GP by embryonic striatal grafts, the present results suggest that the grafts reconstruct the functional striatopallidal pathway which is capable of transmitting apomorphine-induced changes in the neuronal activity.
Nakao
N
N
Department of Neurological Surgery, Department of Neurological Surgery, Wakayama Medical College, 811-1 Kimiidera, Wakayama 641-0012, Japan. nnakao@wakayama-med.ac.jp
Nakai
K
K
Itakura
T
T
D000200:Action Potentials / Q000187:drug effects / Q000502:physiology; D000818:Animals; D001058:Apomorphine / Q000494:pharmacology*; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology; D016380:Brain Tissue Transplantation; D003714:Denervation; D018491:Dopamine Agonists / Q000494:pharmacology*; D004594:Electrophysiology; D016332:Fetal Tissue Transplantation; D005917:Globus Pallidus / Q000166:cytology / Q000502:physiology / Q000637:transplantation*; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D009498:Neurotoxins; D051381:Rats; D017207:Rats, Sprague-Dawley
D018491:Dopamine Agonists; D009498:Neurotoxins; D001058:Apomorphine