Effect of salivary secretory IgA on the adhesion of Candida albicans to polystyrene.
Attachment of Candida albicans to plastic materials of dental prostheses or to salivary macromolecules adsorbed on their surface is believed to be a critical event in the development of denture stomatitis. In an earlier study, it was shown that adhesion of C. albicans to polystyrene, a model system to study the adhesion of C. albicans to plastic materials, can be partially inhibited with an mAb directed against cell wall polysaccharides of C. albicans. In the present study, the role of whole saliva in the adhesion of C. albicans to polystyrene has been investigated, and three mAbs directed against epitopes of cell wall mannoproteins have been used to mimic the inhibitory effect observed with salivary secretory IgA (sIgA) on the adhesion of C. albicans to polystyrene. In the absence of whole saliva, adherence of C. albicans 3153 increased with germination. However, the presence of whole saliva enhanced the adhesion to polystyrene of C. albicans 3153 yeast cells but decreased the adhesion of germinated cells. The enhancement of adhesion of yeast cells to polystyrene mediated by saliva was confirmed with an agerminative mutant of C. albicans 3153. The inhibition of the adhesion of C. albicans 3153 germ tubes to polystyrene was due to the salivary sIgA since sIgA-depleted saliva enhanced the adhesion of C. albicans 3153 to polystyrene. The inhibitory effect mediated by sIgA was not related to the inhibition of germination but to the blockage of adhesins expressed on the cell wall surface of the germ tubes. The three mAbs studied reduced the adhesion of C. albicans 3153 to polystyrene at levels equivalent to those for purified sIgA. The highest reduction in the adhesion was obtained with the IgA mAb N3B. The best results were obtained when the three mAbs were combined. The results suggest that whole saliva plays a different role in the adhesion of C. albicans to polystyrene depending on the morphological phase of C. albicans. These results may give new insights into the conflicting role of saliva in the adhesion of C. albicans to plastic materials of dental prostheses.
San Millán
Rosario
R
Departamento de Inmunologı́a, Microbiologı́a y Parasitologı́a, Facultad de Medicina y Odontologı́a1, and Departamento de Enfermerı́a I2, Universidad del Paı́s Vasco, Apartado 699, E-48080 Bilbao, Vizcaya, Spain.
Elguezabal
Natalia
N
Departamento de Inmunologı́a, Microbiologı́a y Parasitologı́a, Facultad de Medicina y Odontologı́a1, and Departamento de Enfermerı́a I2, Universidad del Paı́s Vasco, Apartado 699, E-48080 Bilbao, Vizcaya, Spain.
Regúlez
Pilar
P
Departamento de Inmunologı́a, Microbiologı́a y Parasitologı́a, Facultad de Medicina y Odontologı́a1, and Departamento de Enfermerı́a I2, Universidad del Paı́s Vasco, Apartado 699, E-48080 Bilbao, Vizcaya, Spain.
Moragues
Marı A Dolores
MAD
Departamento de Inmunologı́a, Microbiologı́a y Parasitologı́a, Facultad de Medicina y Odontologı́a1, and Departamento de Enfermerı́a I2, Universidad del Paı́s Vasco, Apartado 699, E-48080 Bilbao, Vizcaya, Spain.
Quindós
Guillermo
G
Departamento de Inmunologı́a, Microbiologı́a y Parasitologı́a, Facultad de Medicina y Odontologı́a1, and Departamento de Enfermerı́a I2, Universidad del Paı́s Vasco, Apartado 699, E-48080 Bilbao, Vizcaya, Spain.
Pontón
José
J
Departamento de Inmunologı́a, Microbiologı́a y Parasitologı́a, Facultad de Medicina y Odontologı́a1, and Departamento de Enfermerı́a I2, Universidad del Paı́s Vasco, Apartado 699, E-48080 Bilbao, Vizcaya, Spain.
D000911:Antibodies, Monoclonal / Q000276:immunology / Q000502:physiology; D002176:Candida albicans / Q000502:physiology*; D002180:Candidiasis, Oral / Q000382:microbiology; D002448:Cell Adhesion / Q000502:physiology; D002473:Cell Wall / Q000737:chemistry / Q000276:immunology; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000276:immunology / Q000502:physiology*; D008562:Membrane Glycoproteins / Q000276:immunology; D011137:Polystyrenes; D012463:Saliva / Q000502:physiology*
D000911:Antibodies, Monoclonal; D007071:Immunoglobulin A, Secretory; D008562:Membrane Glycoproteins; D011137:Polystyrenes; C025363:mannoproteins