Transformation of Endomyces fibuliger based on its gene for orotidine-5'-phosphate decarboxylase.
Endomyces fibuliger is a yeast used in the production of Chinese rice wine. It secretes enzymes such as glucoamylase, alpha-amylase and acid protease. Very little is known of the genetics of E. fibuliger. In order to develop a transformation system for this yeast, orotidine-5'-phosphate decarboxylase mutant strains were obtained and characterized. Transformation of the E. fibuliger ura3 mutant F1 with an integrative plasmid that carried the wild-type URA3 gene of E. fibuliger gave complementation of this mutation. The E. fibuliger gene encodes the orotidine-5'-phosphate decarboxylase enzyme consisting of 266 amino acid residues with a 69.4% sequence identity with orotidine-5'-phosphate decarboxylase of Saccharomyces cerevisiae. Our finding that E. fibuliger URA3 complements the ura3 mutation in S. cerevisiae confirms that the URA3 gene of E. fibuliger encodes a protein that exerts a similar function.
Ngan
W Y
WY
Department of Microbiology, National University of Singapore, MD4, 5 Science Drive 2, 117597, Singapore, Singapore.
Nga
B H
BH
Pridmore
D
D
Mollet
B
B
Tan
H M
HM
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D005800:Genes, Fungal / Q000235:genetics; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009154:Mutation; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012442:Saccharomycopsis / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014170:Transformation, Genetic
D004271:DNA, Fungal; D009964:Orotidine-5'-Phosphate Decarboxylase