title: Anti-inflammatory activity of dichloromethane extract of Heterotheca inuloides in vivo and in vitro.
abstract: The dichloromethane extract from the dried flowers of Heterotheca inuloides Cass. was investigated on several pharmacological models of inflammation in vivo and in vitro. It showed anti-inflammatory activity on the croton oil-induced oedema test in mouse ear, at 1 mg/ear. The compound isolated from this extract, 7-hydroxy-3,4-dihydrocadalin, showed anti-inflammatory effect on the same experimental model (ED50 of 0.9 mumol/ear), as well as on COX-1 and COX-2 catalysed prostaglandin biosynthesis assays, with IC50 values of 22 microM and 526 microM, respectively. No effect was observed on carrageenan-induced oedema and on fMLP/PAF-induced exocytosis of human neutrophils. The COX-1 inhibitory effect showed by 7-hydroxy-3,4-dihydrocadalin might be related to the anti-inflammatory activity on the topical oedema induced by croton oil.
authors.forename: L
authors.initials: L
authors.lastname: Segura
authors.forename: B
authors.initials: B
authors.lastname: Freixa
authors.forename: T
authors.initials: T
authors.lastname: Ringbom
authors.forename: R
authors.initials: R
authors.lastname: Vila
authors.forename: P
authors.initials: P
authors.lastname: Perera
authors.forename: T
authors.initials: T
authors.lastname: Adzet
authors.forename: L
authors.initials: L
authors.lastname: Bohlin
authors.forename: S
authors.initials: S
authors.lastname: Cañigueral
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry*; D002478:Cells, Cultured; D016861:Cyclooxygenase Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D016861:Cyclooxygenase Inhibitors; D010936:Plant Extracts