Chlorpyrifos-induced hypothermia and vasodilation in the tail of the rat: blockade by scopolamine.
Organophosphate pesticides such as chlorpyrifos reduce core temperature (Tc) in laboratory rodents. The mechanism(s) responsible for the chlorpyrifos-induced hypothermia are not well known. This study assessed the role of a key effector for thermoregulation in the rat, vasomotor control of heat loss from the tail, and its possible cholinergic control during chlorpyrifos-induced hypothermia. Tc and motor activity were monitored by telemetry in female Long-Evans rats maintained at an ambient temperature (Ta) of 25 degrees. Tail skin temperature (Tsk(t)) was measured hourly. Rats were dosed with chlorpyrifos (0 or 25 mg/kg orally). Two hr later the rats were dosed with saline or scopolamine (1.0 mg/kg intraperitoneally). Two hr after chlorpyrifos treatment there was a marked elevation in Tsk(t)) concomitant with a 0.5 degrees reduction in Tc. Scopolamine administered to control rats led to a marked elevation in Tc with little change in Tsk(t). Rats treated with chlorpyrifos and administered scopolamine underwent a marked vasoconstriction and elevation in Tc. Vasodilation of the tail is an important thermoeffector to reduce Tc during the acute stages of chlorpyrifos exposure. The blockade of the response by scopolamine suggests that the hypothermic and vasodilatory response to chlorpyrifos is mediated via a cholinergic muscarinic pathway in the CNS.
Gordon
C J
CJ
Neurotoxicology Division, National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA. gordon.christopher@epa.gov
Yang
Y L
YL
D000704:Analysis of Variance; D000818:Animals; D004390:Chlorpyrifos / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D005260:Female; D007035:Hypothermia / Q000139:chemically induced* / Q000188:drug therapy; D007306:Insecticides / Q000037:antagonists & inhibitors / Q000633:toxicity*; D009043:Motor Activity / Q000187:drug effects; D018727:Muscarinic Antagonists / Q000627:therapeutic use*; D051381:Rats; D020318:Rats, Long-Evans; D012601:Scopolamine / Q000627:therapeutic use*; D012881:Skin Temperature / Q000187:drug effects; D014664:Vasodilation / Q000187:drug effects
D007306:Insecticides; D018727:Muscarinic Antagonists; D012601:Scopolamine; D004390:Chlorpyrifos