Detection of small round structured viruses in stool specimens from outbreaks of gastroenteritis by electron microscopy and reverse transcription-polymerase chain reaction.
In testing 60 stool specimens small round structured virus (SRSV) particles were detected in 35 (58%) specimens from all 17 outbreaks of gastroenteritis by electron microscopy (EM), while SRSV genes were found in 36 (60%) specimens from 15 outbreaks by reverse transcription-polymerase chain reaction (RT-PCR) by use of 2 primer pairs. Specimens from 2 outbreaks were found SRSV particles-positive by EM but SRSV genes-negative by RT-PCR. EM remains the basic examination method for diagnosis of SRSV agents.
Otsu
R
R
Faculty of Health Science, Kyushu University of Health and Welfare, Miyazaki, Japan. rootsu@phoenix.ac.jp
Ishikawa
A
A
Mukae
K
K
D017250:Caliciviridae Infections / Q000453:epidemiology* / Q000821:virology; D004196:Disease Outbreaks; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000453:epidemiology* / Q000821:virology; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008854:Microscopy, Electron; D009663:Norwalk virus / Q000302:isolation & purification*; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012367:RNA, Viral