Phosphorylcholine-containing N-glycans of Trichinella spiralis: identification of multiantennary lacdiNAc structures.
Although the presence of phosphorylcholine (PC) in Trichinella spiralis is well established, the precise structure of the PC-bearing molecules is not known. In this paper, we report structural studies of N-glycans released from T.spiralis affinity-purified antigens by peptide N-glycosidase F. Three classes of N-glycan structures were observed: high mannose type structures; those which had been fully trimmed to the trimannosyl core and were sub-stoichiometrically fucosylated; and those with a trimannosyl core, with and without core fucosylation, carrying between one and eight N-acetylhexosamine residues. Of the three classes of glycans, only the last was found to be substituted with detectable levels of phosphorylcholine.
Morelle
W
W
Department of Biochemistry, Imperial College, London, UK.
Haslam
S M
SM
Olivier
V
V
Appleton
J A
JA
Morris
H R
HR
Dell
A
A
D000107:Acetylation; D000581:Amidohydrolases / Q000378:metabolism; D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry* / Q000378:metabolism; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D008401:Gas Chromatography-Mass Spectrometry; D006858:Hydrofluoric Acid / Q000378:metabolism; D008745:Methylation; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000032:analysis; D009844:Oligosaccharides / Q000737:chemistry / Q000145:classification / Q000378:metabolism; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D010767:Phosphorylcholine / Q000032:analysis*; D011134:Polysaccharides / Q000737:chemistry* / Q000145:classification / Q000378:metabolism; D016339:Spectrometry, Mass, Fast Atom Bombardment; D017160:Trichinella spiralis / Q000737:chemistry*; D014357:Trypsin / Q000378:metabolism
D000947:Antigens, Helminth; D009005:Monosaccharides; D009844:Oligosaccharides; D011134:Polysaccharides; D010767:Phosphorylcholine; D014357:Trypsin; D000581:Amidohydrolases; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D006858:Hydrofluoric Acid