In vitro effects of interleukin-10, prednisolone, and GM-CSF on the non-specific immune function of human polymorphonuclear leucocytes and monocytes.
A wide range of immune-modulating effects make IL-10 a potential therapeutic option in the treatment of numerous diseases pathophysiological based on a dysregulation of cytokine production. The background of this study was to investigate, whether the beneficial effects of a therapeutic immunosuppression with IL-10 may be countered by an increased risk for infections due to impaired effector cell functions of unspecific immunity. We demonstrated the in vitro effects of IL-10 on phagocytosis (P), intracellular killing (K), and chemotactic activity (C) by human neutrophils (PMN) and monocytes (MON) using Candida albicans as test strain and compared the results to the effects of prednisolone and GM-CSF. IL-10 reduced significantly the intracellular killing rate of PMN compared to untreated phagocytes (60 +/- 16% versus 68 +/- 13%, mean +/- SD, p = 0.0002). High dose IL-10 (100 ng/ml) had a stimulating effect on the percentage of phagocytizing MON (70.2 +/- 12.7% vs. 66.9 +/- 14.2%, p = 0.0436), without impairing intracellular killing. Prednisolone reduced significantly the Candida uptake by MON (57 +/- 18.1% vs. 66. 9 +/- 14.2%, p = 0.0019). In contrast to prednisolone, neither MON nor PMN chemotaxis was suppressed by IL-10. In conclusion, IL-10 had only marginal immunosuppressive effects on the unspecific immunity compared to prednisolone.
Ziege
S U
SU
Department of Chest and Infectious Diseases, City Hospital Zehlendorf/Heckeshorn, Berlin, Germany. haloheck@zedat.fu-berlin.de.
Geerdes-Fenge
H F
HF
Rau
M
M
Buchwald
U
U
Lode
H
H
D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002175:Candida / Q000276:immunology; D002633:Chemotaxis / Q000187:drug effects / Q000276:immunology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D016753:Interleukin-10 / Q000494:pharmacology*; D008297:Male; D009000:Monocytes / Q000187:drug effects* / Q000276:immunology*; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology*; D010587:Phagocytosis / Q000187:drug effects / Q000276:immunology; D011239:Prednisolone / Q000494:pharmacology*
D000893:Anti-Inflammatory Agents; D016753:Interleukin-10; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D011239:Prednisolone