The absence of genes for cytochrome c oxidase and reductase subunits in maxicircle kinetoplast DNA of the respiration-deficient plant trypanosomatid Phytomonas serpens.
By completing the sequencing of the maxicircle conserved region in the kinetoplast DNA of Phytomonas serpens, we showed that the genes for subunits I and II (COI and COII) of cytochrome c oxidase in this organism were missing. We had previously shown that the genes for cytochrome c oxidase subunit III and apocytochrome b were also missing. These deletions did not affect the structure or expression of the remaining genes. Partial editing of the mRNA for NADH dehydrogenase subunit 8, previously found in strain IG from insects, was complete in two other strains isolated from plants. The appearance of a novel maxicircle gene for MURF2 block I gRNA, which substitutes for the gene missing due to the COII gene deletion, may illustrate a general mechanism for the origin of gRNAs.
Nawathean
P
P
Department of Biology, University of California, Riverside 92521, USA.
Maslov
D A
DA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004270:DNA, Circular / Q000235:genetics*; D016054:DNA, Protozoan / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D017125:Genes, Protozoan; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics*; D010101:Oxygen Consumption / Q000235:genetics*; D010944:Plants / Q000469:parasitology; D017393:RNA Editing; D017394:RNA, Guide, Kinetoplastida; D012333:RNA, Messenger / Q000235:genetics; D014351:Trypanosomatina / Q000235:genetics*
D004270:DNA, Circular; D016054:DNA, Protozoan; D012333:RNA, Messenger; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV; D017394:RNA, Guide, Kinetoplastida