Assay of the inhibitory effect of pentachlorophenol and formaldehyde on mycelial growth and development of reproductive structures in Aspergillus niger.
The biological activity of the antifungal agents pentachlorophenol (as sodium pentachlorophenolate) and formaldehyde was evaluated by changes in the development of fungal structures following a placement of test strips inoculated with Aspergillus niger conidia on a nutrient agar with the toxicant. The method allows a quantitative assessment of biological activity measured as the development of vegetative structures (growth of the mycelium) by metric, and the development of reproductive structures (conidia) of A. niger by densitometric methods. The use of test strips with dry conidiospores and the evaluation of the results in physical units represent a simple, rapid, exact and inexpensive test of fungitoxic agents.
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BiOMAR (Biotechnology-Microbiology-Analysis-Research), Laboratory for Ecotoxicology, Karlsruhe/Ettlingen, Germany.
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D001234:Aspergillus niger / Q000187:drug effects* / Q000254:growth & development; D003470:Culture Media; D004202:Disinfectants / Q000494:pharmacology*; D005557:Formaldehyde / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000379:methods; D010416:Pentachlorophenol / Q000494:pharmacology*
D003470:Culture Media; D004202:Disinfectants; D005557:Formaldehyde; D010416:Pentachlorophenol