title: [Nucleoli and perinuclear bodies in trophocytes of Panorpa communis contain factors of pre-mRNA splicing].
abstract: The distribution of pre-mRNA splicing factors and protein coilin was examined in trophocyte nuclei (TN) in polytrophic ovarioles of Panorpa communis. In situ hybridization, using antisense U1 and U6 snRNA 3H-riboprobes, showed that TN were labeled evenly. Immunostaining at light and electron microscopic levels revealed in some TN nucleolar structures containing small nuclear RNP (snRNP) and protein coilin characteristic of the Cajal bodies/coiled bodies (CB). No free CBs were found in TN. These data showed that CB in TN are present only in the nucleoli. One of characteristic features of P. communis trophocytes is the presence of several types of perinuclear bodies (PB) in the cytoplasm. We distinguish between three types of PBs. PB-1 consist of spherical bodies (10-20 microns) with vacuoles composed of closely packed fibrils. PB-2 are irregularly shaped bodies (0.3-2.0 microns) consisting of a fibro-granular material. PB-2 are located near the nuclear envelope and contact the nucleoplasm material through nuclear pores. PB-1 and PB-2 join together to form a complex PB of the third type. All types of PB are not surrounded with a membrane and sometimes have mitochondria on their surface. The immunogold technique at the ultrastructural level revealed snRNP in PB-2. These results have enabled us to make a conclusion that PB-2 may be storage sites of snRNPs required for a future development of the embryo.
authors.affiliation: Institute of Cytology, RAS, St. Petersburg.
authors.forename: F M
authors.initials: FM
authors.lastname: Batalova
mesh_terms: D000818:Animals; D002466:Cell Nucleolus / Q000648:ultrastructure; D005260:Female; D007313:Insecta / Q000235:genetics*; D010053:Ovary / Q000648:ultrastructure*; D012322:RNA Precursors / Q000737:chemistry*; D012326:RNA Splicing
chemical_list: D012322:RNA Precursors