title: Directed evolution of an enantioselective lipase.
abstract: BACKGROUND
  The biocatalytic production of enantiopure compounds is of steadily increasing importance to the chemical and biotechnological industry. In most cases, however, it is impossible to identify an enzyme that possesses the desired enantioselectivity. Therefore, there is a strong need to create by molecular biological methods novel enzymes which display high enantioselectivity.
  
  
  RESULTS
  A bacterial lipase from Pseudomonas aeruginosa (PAL) was evolved to catalyze with high enantioselectivity the hydrolysis of the chiral model substrate 2-methyldecanoic acid p-nitrophenyl ester. Successive rounds of random mutagenesis by ep-PCR and saturation mutagenesis resulted in an increase in enantioselectivity from E=1.1 for the wild-type enzyme to E=25.8 for the best variant which carried five amino acid substitutions. The recently solved three-dimensional structure of PAL allowed us to analyze the structural consequences of these substitutions.
  
  
  CONCLUSIONS
  A highly enantioselective lipase was created by increasing the flexibility of distinct loops of the enzyme. Our results demonstrate that enantioselective enzymes can be created by directed evolution, thereby opening up a large area of novel applications in biotechnology.
authors.affiliation: Lehrstuhl für Biologie der Mikroorganismen, Ruhr-Universität, Bochum, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Liebeton
authors.forename: A
authors.initials: A
authors.lastname: Zonta
authors.forename: K
authors.initials: K
authors.lastname: Schimossek
authors.forename: M
authors.initials: M
authors.lastname: Nardini
authors.forename: D
authors.initials: D
authors.lastname: Lang
authors.forename: B W
authors.initials: BW
authors.lastname: Dijkstra
authors.forename: M T
authors.initials: MT
authors.lastname: Reetz
authors.forename: K E
authors.initials: KE
authors.lastname: Jaeger
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D019020:Directed Molecular Evolution / Q000379:methods*; D008049:Lipase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D016296:Mutagenesis; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D011550:Pseudomonas aeruginosa / Q000166:cytology / Q000201:enzymology* / Q000235:genetics; D013237:Stereoisomerism; D013379:Substrate Specificity
chemical_list: D008049:Lipase