A comparison of human S100A12 with MRP-14 (S100A9).
MRP-8 and -14 are two S100 proteins highly expressed as a complex by neutrophils, and to a lesser extent by monocytes and certain squamous epithelia. However, less is known about the close homologue S100A12. This S100 protein is expressed by neutrophils and here we show that it is also expressed by monocytes, but not lymphocytes. An absence of coimmunoprecipitation of MRP-14 and S100A12 indicates that S100A12 is not associated with the MRP proteins in vivo. When directly compared to MRP-14, S100A12 expression by squamous epithelia is more restricted. In esophagus and psoriatic skin, S100A12 is differentially regulated, like MRP-14, but the expression pattern of the two S100 proteins is quite different.
Robinson
M J
MJ
Leukocyte Adhesion Laboratory, Imperial Cancer Research Fund, London, WC2A 3PX, United Kingdom.
Hogg
N
N
D000906:Antibodies / Q000276:immunology; D000943:Antigens, Differentiation / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D040501:Calgranulin A; D040502:Calgranulin B; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004848:Epithelium / Q000378:metabolism; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D007962:Leukocytes / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism; D011233:Precipitin Tests; D011994:Recombinant Proteins / Q000378:metabolism; D009418:S100 Proteins / Q000235:genetics / Q000378:metabolism*; D000067616:S100A12 Protein; D012867:Skin / Q000196:embryology / Q000378:metabolism
D000906:Antibodies; D000943:Antigens, Differentiation; D002135:Calcium-Binding Proteins; D040501:Calgranulin A; D040502:Calgranulin B; D011994:Recombinant Proteins; D009418:S100 Proteins; D000067616:S100A12 Protein; C090441:S100A12 protein, human