title: Pathologic findings and association of Mycobacterium bovis infection with the bovine NRAMP1 gene in cattle from herds with naturally occurring tuberculosis.
abstract: OBJECTIVE
  To determine necropsy and Mycobacterium bovis culture results in cattle from herds with tuberculosis, the role of the bovine NRAMP1 gene in resistance and susceptibility to infection with M bovis, and the association between magnitude of the tuberculous lesions and various types of M bovis isolates.
  
  
  METHODS
  61 cattle from herds with tuberculosis in Texas and Mexico.
  
  
  METHODS
  61 cattle were evaluated by necropsy; 59 had positive and 2 had negative caudal fold tuberculin intradermal test (CFT) results. Thirty-three cattle with positive CFT results were genotyped to evaluate polymorphism of the 3' untranslated region of the bovine NRAMP1 gene, using single-stranded conformational analysis, 9 were resistant to M bovis with no tuberculous lesions and negative M bovis culture results, and 24 were susceptible with tuberculous lesions and positive M bovis culture results. Isolates of M bovis were analyzed by restriction fragment length polymorphism (RFLP) on the basis of IS6110 sequences and direct-repeat fingerprinting patterns.
  
  
  RESULTS
  21 (35.6%; 21/59) cattle with positive CFT results had tuberculous lesions or positive culture results; in addition, 1 of 2 cattle with negative CFT results had tuberculous lesions and positive culture results. Tuberculous lesions were most common in the thorax (35/63; 55.5%) and lymphoid tissues of the head (10/63; 15.9%). Tuberculous lesions varied from 1 to 11/animal; 8 of 21 (38.1%) had solitary lesions. Associations were not found between resistance or susceptibility to infection with M bovis and polymorphism in the NRAMP1 gene or between the magnitude of the lesions and various RFLP types of M bovis isolates.
  
  
  CONCLUSIONS
  The NRAMP1 gene does not determine resistance and susceptibility to infection with M bovis in cattle.
authors.affiliation: Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station 77843-4467, USA.
authors.forename: R
authors.initials: R
authors.lastname: Barthel
authors.forename: J A
authors.initials: JA
authors.lastname: Piedrahita
authors.forename: D N
authors.initials: DN
authors.lastname: McMurray
authors.forename: J
authors.initials: J
authors.lastname: Payeur
authors.forename: D
authors.initials: D
authors.lastname: Baca
authors.forename: F
authors.initials: F
authors.lastname: Suárez Güemes
authors.forename: V S
authors.initials: VS
authors.lastname: Perumaalla
authors.forename: T A
authors.initials: TA
authors.lastname: Ficht
authors.forename: J W
authors.initials: JW
authors.lastname: Templeton
authors.forename: L G
authors.initials: LG
authors.lastname: Adams
mesh_terms: D000483:Alleles; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics*; D027682:Cation Transport Proteins; D002417:Cattle / Q000235:genetics*; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D007113:Immunity, Innate / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics*; D009163:Mycobacterium bovis / Q000302:isolation & purification*; D012150:Polymorphism, Restriction Fragment Length; D018807:Polymorphism, Single-Stranded Conformational; D014380:Tuberculosis, Bovine / Q000235:genetics / Q000473:pathology*; D000099173:Natural Resistance-associated Macrophage Protein 1
chemical_list: D002352:Carrier Proteins; D027682:Cation Transport Proteins; D008565:Membrane Proteins; D000099173:Natural Resistance-associated Macrophage Protein 1