title: Culture of human laryngeal papilloma cells in vitro.
abstract: Human laryngeal epithelial cells have been grown in tissue culture in a hydrated collagen gel containing Nutrient Mixture F12 (Gibco) supplemented with 15% fetal calf serum and 10 micrograms/mL hydrocortisone. Primary cells often remain viable in culture for more than six months. They can be serially transferred two to four times before senescence. Cells derived both from normal epithelium and from laryngeal papilloma have been successfully cultured. Papilloma cells appear to contain more perinuclear granules and form fewer tight junctions than normal cells.
authors.affiliation: Department of Otolaryngology, Long Island Jewish-Hillside Medical Center, New Hyde Park, NY 11042, USA.
authors.forename: B M
authors.initials: BM
authors.lastname: Steinberg
authors.forename: A L
authors.initials: AL
authors.lastname: Abramson
authors.forename: R P
authors.initials: RP
authors.lastname: Meade
mesh_terms: D002455:Cell Division / Q000502:physiology; D046508:Culture Techniques; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology*; D010212:Papilloma / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000473:pathology*