title: Action of the thiamine antagonist bacimethrin on thiamine biosynthesis.
abstract: Bacimethrin is an analog of the 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP) moiety of thiamine and inhibits the growth of Salmonella enterica serovar Typhimurium on a defined medium. Two classes of mutants that had increased bacimethrin resistance were isolated and characterized. Results showed that overexpression of the thi operon or specific lesions in thiD resulted in a bacimethrin-resistant phenotype. Phenotypic analyses of the thiD mutants suggested that they had a specific defect in one of the two kinase activities associated with this gene product and, further, that ThiD and not PdxK was primarily responsible for salvage of HMP from the medium.
authors.affiliation: Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
authors.forename: J L
authors.initials: JL
authors.lastname: Zilles
authors.forename: L R
authors.initials: LR
authors.lastname: Croal
authors.forename: D M
authors.initials: DM
authors.lastname: Downs
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D002352:Carrier Proteins; D004352:Drug Resistance, Microbial / Q000235:genetics; D029968:Escherichia coli Proteins; D016296:Mutagenesis; D009713:Nucleotidyltransferases / Q000235:genetics; D009876:Operon; D010641:Phenotype; D017856:Phosphotransferases (Phosphate Group Acceptor) / Q000235:genetics / Q000378:metabolism; D011743:Pyrimidines / Q000378:metabolism* / Q000494:pharmacology; D019779:Salmonella enterica / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D013831:Thiamine / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D011743:Pyrimidines; C105797:ThiC protein, Bacteria; C411655:thiS protein, E coli; C054356:bacimethrin; C108436:4-amino-5-(hydroxymethyl)-2-methylpyrimidine phosphate kinase; D017856:Phosphotransferases (Phosphate Group Acceptor); D009713:Nucleotidyltransferases; C411654:ThiF protein, E coli; D013831:Thiamine