Three functionally diverged major structural proteins of white spot syndrome virus evolved by gene duplication.
White spot syndrome virus (WSSV) is an invertebrate virus causing considerable mortality in penaeid shrimp. The oval-to-bacilliform shaped virions, isolated from infected Penaeus monodon, contain four major proteins: VP28, VP26, VP24 and VP19 (28, 26, 24 and 19 kDa, respectively). VP26 and VP24 are associated with the nucleocapsid and the remaining two with the envelope. Forty-one N-terminal amino acids of VP24 were determined biochemically allowing the identification of its gene (vp24) in the WSSV genome. Computer-assisted analysis revealed a striking similarity between WSSV VP24, VP26 and VP28 at the amino acid and nucleotide sequence level. This strongly suggests that these structural protein genes may have evolved by gene duplication and subsequently diverged into proteins with different functions in the WSSV virion, i.e. envelope and nucleocapsid. None of these three structural WSSV proteins showed homology to proteins of other viruses including baculoviruses, underscoring the distinct taxonomic position of WSSV among invertebrate viruses.
van Hulten
Mariëlle C W
MCW
Laboratory of Virology, Wageningen University, Binnenhaven 11, 6709 PD Wageningen, The Netherlands1.
Goldbach
Rob W
RW
Laboratory of Virology, Wageningen University, Binnenhaven 11, 6709 PD Wageningen, The Netherlands1.
Vlak
Just M
JM
Laboratory of Virology, Wageningen University, Binnenhaven 11, 6709 PD Wageningen, The Netherlands1.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004267:DNA Viruses; D033364:Decapoda / Q000821:virology*; D004591:Electrophoresis, Polyacrylamide Gel; D020440:Gene Duplication; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D015678:Viral Structural Proteins / Q000235:genetics*
D015678:Viral Structural Proteins