Short bowel syndrome: experimental approach to increase intestinal surface in rats by gastric homologous acellular matrix.
OBJECTIVE
In this preliminary work the authors used homologous acellular matrix obtained by the gastric wall to increase the small bowel surface in Sprague-Downey rats; through this experimental model the authors verified that homologous acellular matrix can support cell migration and the reconstruction of the intestinal wall.


METHODS
A tract of about 2 cm of tubular gastric acellular matrix was inserted with bilateral anastomosis in an isolated ileal loop, which was located in endoabdominal position through a short subcutaneous tunnel. Twelve animals were analyzed at each of the time-points ranging from 1 to 6 weeks after surgery.


RESULTS
Histologic evaluation showed that the implanted matrix can be reintegrated in the normal small bowel in a period ranging between 3 and 6 weeks from surgery. The implanted matrix was organized with 4 different tonacae from the third week after the surgery, without interruption at the site of the anastomosis.


CONCLUSIONS
To date, the authors do not have a demonstration of the function of the ileal loop reconstructed with this technique; based on these results the authors are engaged in an experimental trial of restoration of intestinal viability with the ileal prosthesis after 3 weeks to study its function.
Parnigotto
P P
PP
Department of Pharmaceutical Sciences, University of Padua, Italy.
Marzaro
M
M
Artusi
T
T
Perrino
G
G
Conconi
M T
MT
D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use*; D007081:Ileostomy / Q000379:methods*; D007082:Ileum / Q000502:physiology; D008297:Male; D019919:Prosthesis Implantation / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration; D012778:Short Bowel Syndrome / Q000601:surgery*; D014184:Transplantation, Homologous
D001672:Biocompatible Materials