title: Effects of berberine on arylamine N-acetyltransferase activity and 2-aminofluorene-DNA adduct formation in human leukemia cells.
abstract: Berberine is an alkaloid occurring in the plant genera Berberis and Coptis. Although berberine had been demonstrated to have antineoplastic function by inhibiting DNA-synthesis in activated lymphocytes, there is no available information to address berberine affects on human leukemia cell N-acetyltransferase (NAT) activity and 2-aminofluorene (AF)-DNA adduct formation. Thus, berberine was tested for inhibition of arylamine NAT activity and AF-DNA adduct formation in human leukemia cells. The NAT activity was measured by a high performance liquid chromatography assaying for the amounts of N-acetyl-2-aminofluorene (AAF) and N-acetyl-p-aminobenzoic acid (N-Ac-PABA) and the remaining AF and p-aminobenzoic acid (PABA). The NAT activity and AF-DNA adduct formation in human leukemia cells were inhibited by berberine in a dose-dependent manner, i.e. the higher the concentration of berberine, the higher the inhibition of NAT activity and AF-DNA adduct. The data also indicate that berberine decreased the apparent values of Km and Vmax from human leukemia cells in both cytosol and intact cells.
authors.affiliation: Department of Microbiology, China Medical College, Taichung, Taiwan.
authors.forename: J G
authors.initials: JG
authors.lastname: Chung
authors.forename: G W
authors.initials: GW
authors.lastname: Chen
authors.forename: C F
authors.initials: CF
authors.lastname: Hung
authors.forename: J H
authors.initials: JH
authors.lastname: Lee
authors.forename: C C
authors.initials: CC
authors.lastname: Ho
authors.forename: H C
authors.initials: HC
authors.lastname: Ho
authors.forename: H L
authors.initials: HL
authors.lastname: Chang
authors.forename: W C
authors.initials: WC
authors.lastname: Lin
authors.forename: J G
authors.initials: JG
authors.lastname: Lin
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D001191:Arylamine N-Acetyltransferase / Q000187:drug effects*; D001599:Berberine / Q000494:pharmacology*; D002273:Carcinogens / Q000737:chemistry / Q000378:metabolism*; D018736:DNA Adducts / Q000737:chemistry / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D005449:Fluorenes / Q000737:chemistry / Q000378:metabolism*; D018922:HL-60 Cells / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D006801:Humans; D007951:Leukemia, Myeloid / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology
chemical_list: D000970:Antineoplastic Agents; D002273:Carcinogens; D018736:DNA Adducts; D004365:Drugs, Chinese Herbal; D005449:Fluorenes; D001599:Berberine; C012177:2-aminofluorene; D001191:Arylamine N-Acetyltransferase