title: [Interaction of certain RNAases and mannose-specific lectins].
abstract: While defining and elaborating the approaches to examination of the interaction between C-mannosylated tryptophan, recently discovered in the laboratory of Dr. Jan Hofsteenge, and Man/Glc specific lectins the unexpected results were obtained. Some animal origin mannosyl-containing RNases (as was expected) as well as analogous but nonglycosylated recombinant proteins expressed in E. coli (a negative control) were recognized by the mentioned lectins. Protein-protein interactions between lectins and expressed in E. coli nonglycosylated RNases are supposed and require further investigations.
authors.affiliation: Institute of Biochemistry, National Academy of Sciences of Ukraine.
authors.forename: O S
authors.initials: OS
authors.lastname: Miroshnichenko
mesh_terms: D002846:Chromatography, Affinity; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008358:Mannose / Q000378:metabolism*; D011485:Protein Binding; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism*; D014364:Tryptophan / Q000378:metabolism
chemical_list: D037102:Lectins; D011994:Recombinant Proteins; D014364:Tryptophan; D012260:Ribonucleases; D008358:Mannose