Linker-mediated modulation of the chemiluminescent signal from N(10)-(3-Sulfopropyl)-N-sulfonylacridinium-9-carboxamide tracers.
Four chemiluminescent N-sulfonylacridinium-9-carboxamide active esters (17-20) were prepared from the corresponding acids and coupled to both of the aminated phenobarbital (13) and N-(6-aminohexyl)phenytoin (16) haptens. The level of signal produced by chemiluminescent N-sulfonylacridinium-9-carboxamide phenobarbital and phenytoin tracers in a solid-phase immunoassay format was found to be modulated by at least 20-fold by the judicious choice of the reactive acridinium-hapten linking group.
Adamczyk
M
M
Abbott Laboratories, Diagnostics Division, Department of Chemistry D9NM, Building AP 20, 100 Abbott Park Road, Abbott Park, Illinois 60064-6016, USA. maciej.adamczyk@abbott.com
Chen
Y Y
YY
Fishpaugh
J R
JR
Mattingly
P G
PG
Pan
Y
Y
Shreder
K
K
Yu
Z
Z
D000166:Acridines / Q000138:chemical synthesis / Q000737:chemistry*; D000818:Animals; D000906:Antibodies; D001666:Binding Sites, Antibody; D006241:Haptens; D007118:Immunoassay / Q000379:methods; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D015394:Molecular Structure; D010634:Phenobarbital / Q000031:analogs & derivatives / Q000032:analysis*; D010672:Phenytoin / Q000031:analogs & derivatives / Q000032:analysis*; D012680:Sensitivity and Specificity; D012756:Sheep; D013329:Structure-Activity Relationship
D000166:Acridines; D000906:Antibodies; D006241:Haptens; D007202:Indicators and Reagents; C415762:N(10)-(3-sulfopropyl)-N-sulfonylacridinium-9-carboxamide; D010672:Phenytoin; D010634:Phenobarbital