[Molecular cloning and nucleotide sequence of soybean glycinin gene Gy5(A3B4)].
The glycinin gene family encoding the glycinin subunits in soybean plants is composed of at least 5 gene members, i.e.: Gy1-Gy5. A genomic clone containing the Gy5 gene from a genomic library of cv. Williams was isolated by using Gy5 cDNA probe. The complete nucleotide sequence of this gene has been determined. It is 2819 bp long consisting of four exons and three introns. These exons and introns are as follows: exonl(292 bp), intronl(358 bp), exon2(263 bp), intron2(425 bp), exon3(645 bp), intron3(485 bp), exon4(351 bp). The gene encodes 517 amino asids. This is the first time to report the complete Gy5 gene sequence from a genomic library.
Chen
S F
SF
Dept. of Microbiology, China Agricultural University, Beijing.
Fukazawa
C
C
D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry*; D005091:Exons; D005916:Globulins / Q000235:genetics*; D007438:Introns; D008969:Molecular Sequence Data; D030262:Soybean Proteins
D005916:Globulins; D030262:Soybean Proteins; D004247:DNA; C011474:glycinin