Supernatants from leucocytes treated with melanin-concentrating hormone (MCH) and alpha-melanocyte stimulating hormone (alpha-MSH) have a stimulatory effect on rainbow trout (Oncorhynchus mykiss) phagocytes in vitro.
Melanin-concentrating hormone (MCH) and alpha-melanocyte stimulating hormone (alpha-MSH) are widespread vertebrate neuropeptides. In teleost fish the peptides are involved in the hormonal control of skin pigmentation, but they have also been shown to modulate corticosteroid secretion in both fish and mammals. alpha-MSH has additional potent anti-inflammatory actions in mammals and both peptides stimulate leucocyte phagocytosis in rainbow trout in vitro. The effects of these peptides on phagocytosis and the release of immunomodulatory factors by rainbow trout head kidney leucocytes were investigated in vitro. Neither MCH nor alpha-MSH had any effect on the adherence of phagocytes to glass slides or the activity of isolated phagocytes. When added to mixed leucocyte suspensions, however, MCH (50 and 100nM) and alpha-MSH (1 and 10nM) significantly increased the percentage of cells undergoing phagocytosis and MCH (50nM), but not alpha-MSH, stimulated the phagocytic index. In subsequent experiments, isolated phagocytes were exposed to supernatants derived from mixed leucocyte suspensions exposed to MCH (50 and 100nM) and alpha-MSH (1 and 10nM). Supernatants from leucocytes exposed to all doses of the peptides significantly increased the percentage phagocytosis and those from cells stimulated with MCH (100nM) and alpha-MSH (1 and 10nM) increased the phagocytic index of the phagocytes. The results suggest that cells other than phagocytes are required for MCH and alpha-MSH to exert their stimulatory effects on trout phagocytic cells through the release of one or more macrophage-activating factors.
Harris
J
J
Faculty of Applied Sciences, University of the West of England, Bristol, BS16 1QY UK. james.harris@bbsrc.ac.uk
Bird
D J
DJ
D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D007028:Hypothalamic Hormones / Q000494:pharmacology*; D007668:Kidney / Q000166:cytology / Q000276:immunology; D007962:Leukocytes / Q000187:drug effects*; D008543:Melanins / Q000494:pharmacology*; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D010586:Phagocytes / Q000187:drug effects*; D010907:Pituitary Hormones / Q000494:pharmacology*; D000521:alpha-MSH / Q000494:pharmacology*
D007028:Hypothalamic Hormones; D008543:Melanins; D010907:Pituitary Hormones; D000521:alpha-MSH; C019463:melanin-concentrating hormone