title: A method to estimate acetylcholinesterase-active sites and turnover in insects.
abstract: Acetylcholinesterase is the primary target of organophosphorous and carbamate insecticides. Quantitative changes in acetylcholinesterase are suspected to confer resistance to these insecticides, but a method to estimate the amount in insect is not available. A method using irreversible inhibitors has been developed. Among the irreversible inhibitors tested, 7-(methylethoxyphosphinyloxy)-1-methylquinolinium iodide, chlorpyrifos-ethyl-oxon, and coumaphos-oxon were found to be sufficiently potent and specific.
authors.affiliation: Laboratoire de Synthèse et Physicochimie des Molécules d' Intérêt Biologiques, Université Paul Sabatier, Toulouse, 31062, France.
authors.forename: A
authors.initials: A
authors.lastname: Charpentier
authors.forename: P
authors.initials: P
authors.lastname: Menozzi
authors.forename: V
authors.initials: V
authors.lastname: Marcel
authors.forename: F
authors.initials: F
authors.lastname: Villatte
authors.forename: D
authors.initials: D
authors.lastname: Fournier
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D002800:Cholinesterase Inhibitors / Q000378:metabolism; D007313:Insecta / Q000378:metabolism*; D007305:Insecticide Resistance; D007306:Insecticides / Q000378:metabolism
chemical_list: D002800:Cholinesterase Inhibitors; D007306:Insecticides; D000110:Acetylcholinesterase