Ultradian oscillation of Saccharomyces cerevisiae during aerobic continuous culture: hydrogen sulphide mediates population synchrony.
Saccharomyces cerevisiae showed an ultradian respiratory oscillation during aerobic continuous culture. Analysis of the off-gas revealed that hydrogen sulphide production also oscillated. Production was first detected at the onset of low respiration and reached a maximum (1.5 microM) prior to minimum respiratory activity. Then H(2)S concentration fell rapidly to below 0.2 microM before the onset of high respiration. Injection of respiratory oscillation perturbation agents, such as glutathione (50 microM), NaNO(2) (50 microM) or acetaldehyde (4.5 mM),() transiently increased H(2)S production above 6 microM. The synchronization properties of H(2)S were analysed to reveal that changes of oscillation period and amplitude were dependent on H(2)S concentration in culture. It is concluded that H(2)S produced during oscillation produces population synchrony by respiratory chain inhibition.
Sohn
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Biochemical Engineering Laboratory, National Institute of Bioscience and Human Technology, 1-1, Higashi. Tsukuba, Ibaraki 305-8566, Japan.
Murray
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Kuriyama
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D000079:Acetaldehyde / Q000494:pharmacology; D001683:Biological Clocks / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000494:pharmacology; D006862:Hydrogen Sulfide / Q000378:metabolism; D010101:Oxygen Consumption / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D012977:Sodium Nitrite / Q000494:pharmacology; D013440:Sulfides / Q000494:pharmacology
D013440:Sulfides; D005978:Glutathione; D000079:Acetaldehyde; D012977:Sodium Nitrite; C033479:sodium sulfide; D006862:Hydrogen Sulfide