title: Determination of N,N-dimethylaminoethyl chloride and the dimethylaziridinium ion at sub-ppm levels in diltiazem hydrochloride by LC-MS with electrospray ionisation.
abstract: An LC-MS method is described for the determination of the synthetic reagent N,N-dimethylaminoethyl chloride (DMC) in the drug substance diltiazem hydrochloride, for which the permissible limit is not more than 1 ppm (microgram g-1). The N,N-dimethylaziridinium ion (DMA), the reactive intermediate formed by cyclisation of DMC, is also detected. A column switching arrangement is used: diltiazem hydrochloride is trapped on a reversed-phase HPLC column, and the polar analytes are separated by ion exchange chromatography. Ionisation is effected by positive-ion electrospray, and the quadrupole filter mass spectrometer is operated in the selected ion recording mode. The detection limit (peak height-to-baseline noise ratio = 3) for DMC varies from day to day in the range < 0.05 to 0.1 ppm. The response for DMC is linear (r > 0.999) over the concentration range 0.2-10 ppm, and the repeatability is better than 7% (relative standard deviation) at 1.0 ppm. Concentrations of DMC in diltiazem hydrochloride from the manufacturing facility under study ranged from undetectable to about 0.07 ppm. An indirect TLC method has been published for the determination of DMC in mepyramine maleate, but it lacks the necessary sensitivity and specificity. The LC-MS method presented is direct, straightforward and suitable for routine use.
authors.affiliation: Analytical Development Department, Sanofi-Synthélabo, Longjumeau, France.
authors.forename: C R
authors.initials: CR
authors.lastname: Lee
authors.forename: M
authors.initials: M
authors.lastname: Hubert
authors.forename: C N
authors.initials: CN
authors.lastname: Van Dau
authors.forename: D
authors.initials: D
authors.lastname: Peter
authors.forename: A M
authors.initials: AM
authors.lastname: Krstulovic
mesh_terms: D000477:Alkylating Agents / Q000032:analysis*; D001388:Aziridines / Q000032:analysis; D002121:Calcium Channel Blockers / Q000737:chemistry*; D002852:Chromatography, Ion Exchange; D004110:Diltiazem / Q000737:chemistry*; D004340:Drug Contamination; D005021:Ethylamines / Q000032:analysis*; D013058:Mass Spectrometry
chemical_list: D000477:Alkylating Agents; D001388:Aziridines; D002121:Calcium Channel Blockers; D005021:Ethylamines; C114993:dimethylaminoethyl chloride; D004110:Diltiazem