Resolution of benzo[a]pyrene in complex mixtures of other polycyclic aromatic hydrocarbons. Comparison of two spectrofluorimetric methods applied to water samples.
Two spectrofluorimetric methods, second-derivative constant-energy synchronous luminescence (SDCESL) and constant-wavelength synchronous luminescence (CWSL) in combination with multiple linear regression (MLR), for the quantification of benzo[a]pyrene (BaP) at sub-ng mL-1 levels, in the presence of benzo[b]fluoranthene (BbFt), benzo[k]fluoranthene (BkFt), benzo[ghi]perylene (BghiP) and indeno[1,2,3-cd]pyrene (IP), were developed and compared in detail. SDCESL presents lower limits of detection and quantification than CWSL/MLR and also gives more exact and precise results for levels close to the quantification limit. For BaP, SDCESL achieved quantification limits of 0.019 ng mL-1 in river waters and 0.007 ng mL-1 in drinking waters. This work offers a sensitive, precise, accurate, rapid, simple and economic methodology for monitoring BaP in waters for public consumption, meeting all the requirements of the EC Directive 98/83/CE that fixes the maximum admissible limit for this polycyclic aromatic hydrocarbon in drinking waters at of 0.010 ng mL-1.
Andrade Eiroa
A
A
Department of Analytical Chemistry, Coruña University, Zapateira, Spain.
Vázquez Blanco
E
E
López Mahía
P
P
Muniategui Lorenzo
S
S
Prada Rodríguez
D
D
D001564:Benzo(a)pyrene / Q000302:isolation & purification*; D002273:Carcinogens / Q000302:isolation & purification*; D006841:Hydrocarbons, Aromatic / Q000032:analysis; D016014:Linear Models; D013050:Spectrometry, Fluorescence / Q000379:methods; D014874:Water Pollutants, Chemical / Q000302:isolation & purification*
D002273:Carcinogens; D006841:Hydrocarbons, Aromatic; D014874:Water Pollutants, Chemical; D001564:Benzo(a)pyrene