HLA-B27 expression by flow cytometry: an analysis of 7 years quality assurance data.
The Royal College of Pathologists of Australasia Quality Assurance Programs Pty. Ltd. has been monitoring HLA-B27 assignment by flow cytometry for 7 years as part of the Immunology Program. Here we present data that demonstrates a gradual improvement in reports of false positive and negative results. Many participating laboratories demonstrate an ability to assign HLA-B27 status correctly by flow cytometric means. This ability appears to be independent of reagent and methodology. However a small number of laboratories produce consistently unacceptable results that suggest poor quality assurance practice.
Macardle
P J
PJ
Department of Immunology, Allergy and Arthritis, Flinders Medical Centre, Adelaide SA5047, Bedford Park, Australia. peter.macardle@flinders.edu.au
McEvoy
R
R
Jovanovich
S
S
D005434:Flow Cytometry / Q000379:methods*; D015796:HLA-B27 Antigen / Q000032:analysis*; D006801:Humans; D007753:Laboratories / Q000592:standards; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D011786:Quality Control; D015203:Reproducibility of Results
D015796:HLA-B27 Antigen