Bub3 gene disruption in mice reveals essential mitotic spindle checkpoint function during early embryogenesis.
Bub3 is a conserved component of the mitotic spindle assembly complex. The protein is essential for early development in Bub3 gene-disrupted mice, evident from their failure to survive beyond day 6.5-7.5 postcoitus (pc). Bub3 null embryos appear normal up to day 3.5 pc but accumulate mitotic errors from days 4.5-6.5 pc in the form of micronuclei, chromatin bridging, lagging chromosomes, and irregular nuclear morphology. Null embryos treated with a spindle-depolymerising agent fail to arrest in metaphase and show an increase in mitotic disarray. The results confirm Bub3 as a component of the essential spindle checkpoint pathway that operates during early embryogenesis.
Kalitsis
P
P
Murdoch Children's Research Institute, Royal Children's Hospital, Flemington Road, Parkville 3052, Melbourne, Australia.
Earle
E
E
Fowler
K J
KJ
Choo
K H
KH
D000818:Animals; D018797:Cell Cycle Proteins; D002678:Chimera; D002868:Chromosomal Proteins, Non-Histone; D005314:Embryonic and Fetal Development; D051379:Mice; D008810:Mice, Inbred C57BL; D008938:Mitosis; D016254:Mutagenesis, Insertional; D015739:Nocodazole / Q000494:pharmacology; D000075223:Poly-ADP-Ribose Binding Proteins; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D008941:Spindle Apparatus / Q000235:genetics* / Q000378:metabolism
C489445:Bub3 protein, mouse; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D000075223:Poly-ADP-Ribose Binding Proteins; D011506:Proteins; D015739:Nocodazole