Poly(DMAEMA-NVP)-b-PEG-galactose as gene delivery vector for hepatocytes.
A block copolymer composed of cationic polymer and poly(ethylene glycol) (PEG) was used as a DNA carrier. Poly(2-(dimethylamino)ethyl methacrylate (DMAEMA)-co-N-vinyl-2-pyrrolidone (NVP)) having a terminal carboxylic group was synthesized by free radical polymerization using an initiator, 4,4'-azobis(4-cyanovaleric acid). The terminal carboxylic acid was activated by N-hydroxysuccinimide (NHS) with dicyclohexylcarbodiimide (DCC) and then conjugated with PEG-bis(amine). For specific gene targeting to asialoglycoprotein receptor of hepatocytes, a galactose moiety was incorporated into the PEG terminal end of poly(DMAEMA-NVP)-b-PEG by reductive coupling using lactose and sodium cyanoborohydride. RSV luciferase plasmid was used as a reporter gene, and in vitro gene transfection efficiency was measured in HepG2 human hepatocarcinoma cells. Poly(DMAEMA-NVP)-b-PEG-galactose/DNA complexes formed at 0.5-2 polymer/plasmid weight ratio had compacted structures around 200 nm particle size and exhibited slightly negative surface charge. These complexes were coated with a cationic, pH sensitive, endosomolytic peptide, KALA, to generate positively charged poly(DMAEMA-NVP)-b-PEG-galactose/DNA/KALA complex particles. In the presence of serum proteins, both the PEG block and the galactose moiety of poly(DMAEMA-NVP)-b-PEG-galactose greatly enhanced the gene transfection efficiency, which was very close to that of Lipofectamine plus. Irrespective of the presence of serum proteins, as the KALA/DNA weight ratio increased, the transfection efficiency of poly(DMAEMA-NVP)-b-PEG-galactose was enhanced due to the pH dependent endosomal disruptive property of KALA. This study demonstrates that sufficient transfection efficiency as high as that of commercial agent could be attained by judicious formulation of molecular engineered poly(DMAEMA-NVP)-b-PEG-galactose in combination with an endosomolytic peptide, KALA.
Lim
D W
DW
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejon 305-701, South Korea.
Yeom
Y I
YI
Park
T G
TG
D000595:Amino Acid Sequence; D006528:Carcinoma, Hepatocellular; D002411:Cation Exchange Resins; D002470:Cell Survival / Q000187:drug effects; D017930:Genes, Reporter; D005822:Genetic Vectors; D022781:Hepatocytes / Q000378:metabolism*; D006801:Humans; D007202:Indicators and Reagents; D008055:Lipids; D008113:Liver Neoplasms; D008156:Luciferases / Q000235:genetics; D008958:Models, Molecular; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D010957:Plasmids / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000138:chemical synthesis* / Q000737:chemistry / Q000633:toxicity; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry / Q000633:toxicity; D011199:Potentiometry; D011760:Pyrrolidinones / Q000138:chemical synthesis* / Q000737:chemistry / Q000633:toxicity; D013499:Surface Properties; D014162:Transfection / Q000379:methods*; D014407:Tumor Cells, Cultured
D002411:Cation Exchange Resins; D007202:Indicators and Reagents; D008055:Lipids; C086724:Lipofectamine; D010455:Peptides; D011108:Polymers; D011760:Pyrrolidinones; C415749:poly(DMAEMA-NVP)-b-PEG-galactose; D011092:Polyethylene Glycols; D008156:Luciferases