Coordinate regulation of the IL-4, IL-13, and IL-5 cytokine cluster in Th2 clones revealed by allelic expression patterns.
The cytokines IL-4, IL-13, and IL-5 are markers for the Th2 subset of effector T cells and are often expressed together. These cytokine genes are organized within 140 kb of orthologous DNA in both mouse and human. Using IL-4-expressing CD4+ T cell clones derived from F1 mice, we identified allelic polymorphisms for each of these cytokines and assessed the parental identity of the cytokine mRNAs. Both monoallelic and biallelic expression occurred for each gene and for an additional gene, IL-3, that lies with GM-CSF over 450 kb telomeric on the same chromosome. When coexpressed in T cell clones, IL-4 was expressed from the same allele as IL-13 or IL-5 in 81% of instances. In contrast, there was only 52% concordance of these three cytokines at the allelic level among clones that expressed IL-3. Independent expression of the cytokine alleles occurs commonly in T cells, but the clustered locus encompassing IL-4, IL-13, and IL-5 is subject to coordinate regulation.
Kelly
B L
BL
Howard Hughes Medical Institute and Departments of Medicine and Microbiology/Immunology, University of California, San Francisco, CA 93143, USA.
Locksley
R M
RM
D000483:Alleles; D000818:Animals; D002875:Chromosomes / Q000276:immunology; D002999:Clone Cells; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics*; D018984:Epitopes, T-Lymphocyte / Q000235:genetics; D005786:Gene Expression Regulation / Q000276:immunology*; D008040:Genetic Linkage / Q000276:immunology; D018793:Interleukin-13 / Q000096:biosynthesis / Q000235:genetics; D015847:Interleukin-4 / Q000096:biosynthesis / Q000235:genetics; D015848:Interleukin-5 / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D005810:Multigene Family / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology* / Q000378:metabolism*
D016207:Cytokines; D018984:Epitopes, T-Lymphocyte; D018793:Interleukin-13; D015848:Interleukin-5; D015847:Interleukin-4