Instability at dinucleotide and trinucleotide repeats in breast cancer.
We analyzed 7 mono-, 6 di- and 2 trinucleotide repeat loci in a well characterized series of 69 breast cancer cases, treated in the period 1985-1986 and followed for 12 years. Tumor-associated allele contractions or expansions were observed only at di- and trinucleotide repeats, and were detected in 14/69 cases (20%), of which 7 (10%) showed instability at 2 or more loci (10%). No alterations were detected at mononucleotide repeats known to be unstable in gastrointestinal tumors with the microsatellite mutator phenotype. Disease-free survival at 5 years, overall survival at 12 years of follow-up, tumor stage, estrogen/progesteron receptor status, and expression of the Ki-67 proliferation marker were independent of microsatellite status.
Ottini
L
L
Department of Oncology and Neurosciences, University <Gabriele D'Annunzio>, 66013 Chieti, Italy.
Palli
D
D
Falchetti
M
M
D'Amico
C
C
Noviello
C
C
Saieva
C
C
Russo
A
A
Corsi
A M
AM
Masala
G
G
Paglierani
M
M
Vezzosi
V
V
Bianchi
S
S
Mariani-Costantini
R
R
D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D004247:DNA / Q000235:genetics; D018900:Dinucleotide Repeats / Q000235:genetics*; D005260:Female; D005500:Follow-Up Studies; D005838:Genotype; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis; D008875:Middle Aged; D010641:Phenotype; D011960:Receptors, Estrogen / Q000032:analysis; D011980:Receptors, Progesterone / Q000032:analysis; D016019:Survival Analysis; D018911:Trinucleotide Repeats / Q000235:genetics*
D019394:Ki-67 Antigen; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D004247:DNA