title: An animal model of acute otitis media consequent to beta-lactamase-producing nontypable Haemophilus influenzae.
abstract: A chinchilla model of acute otitis media with effusion consequent to beta-lactamase-producing nontypable Haemophilus influenzae was developed using the method of direct inoculation of 145 colony-forming units (CFU) or 252 CFU of beta-lactamase-producing nontypable H influenzae into the right superior bullae of 40 chinchillas. The course of the disease was documented longitudinally by otomicroscopy, tympanometry, and periodic culturing of the middle ears. Onset of the disease occurred in 100% of the animals between two and six days postinoculation and resolution was complete in all ears by day 36. Results of rechallenge with the same organism support the combined effect of a local and weaker systemic middle ear protective mechanism rendering resistance to reinfection with a homologous organism in the chinchilla.
authors.affiliation: Department of Otolaryngology, University of Pittsburgh School of Medicine, PA, USA.
authors.forename: W J
authors.initials: WJ
authors.lastname: Doyle
authors.forename: J S
authors.initials: JS
authors.lastname: Supance
authors.forename: G
authors.initials: G
authors.lastname: Marshak
authors.forename: E I
authors.initials: EI
authors.lastname: Cantekin
authors.forename: C D
authors.initials: CD
authors.lastname: Bluestone
authors.forename: D D
authors.initials: DD
authors.lastname: Rohn
mesh_terms: D000208:Acute Disease; D000668:Ampicillin Resistance; D000818:Animals; D001431:Bacteriological Techniques; D002682:Chinchilla; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D006192:Haemophilus Infections / Q000188:drug therapy / Q000382:microbiology*; D006193:Haemophilus influenzae / Q000187:drug effects / Q000201:enzymology*; D010034:Otitis Media with Effusion / Q000188:drug therapy / Q000382:microbiology*; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D001618:beta-Lactamases