Functional expression of Cre recombinase in sub-regions of mouse CNS and retina.
We describe a strategy for generating CNS and retina sub-region-specific mutations using the Cre/loxP system. Transgenic mice expressing Cre recombinase under the control of the c-kit promoter were established. Functional Cre expression was predominantly found to be restricted to the CA1, CA2 and CA3 regions of the hippocampus, the anterior region of the dentate gyrus, and to the ganglion cell layer of the retina.
Eriksson
B
B
Department of Cell and Molecular Biology, Umeå University, Sweden. bjorn.eriksson@cmb.umu.se
Bergqvist
I
I
Eriksson
M
M
Holmberg
D
D
D000818:Animals; D015139:Blotting, Southern; D018891:Dentate Gyrus / Q000378:metabolism* / Q000473:pathology; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D019426:Integrases / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D008957:Models, Genetic; D016296:Mutagenesis; D011401:Promoter Regions, Genetic; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics; D011995:Recombination, Genetic; D012165:Retinal Ganglion Cells / Q000378:metabolism* / Q000473:pathology; D013997:Time Factors; D014018:Tissue Distribution; D014764:Viral Proteins
D014764:Viral Proteins; D019009:Proto-Oncogene Proteins c-kit; C045073:Cre recombinase; D019426:Integrases