title: Lymphoid/neuronal cell surface OX2 glycoprotein recognizes a novel receptor on macrophages implicated in the control of their function.
abstract: The OX2 membrane glycoprotein (CD200) is expressed on a broad range of tissues including lymphoid cells, neurons, and endothelium. We report the characterization of an OX2 receptor (OX2R) that is a novel protein restricted to cells of the myeloid lineage. OX2 and its receptor are both cell surface glycoproteins containing two immunoglobulin-like domains and interact with a dissociation constant of 2.5 microM and koff 0.8 s(-1), typical of many leukocyte protein membrane interactions. Pervanandate treatment of macrophages showed that OX2R could be phosphorylated on tyrosine residues. Blockade of the OX2-OX2R interaction with an OX2R mAb exacerbated the disease model experimental allergic encephalomyelitis. These data, together with data from an OX2-deficient mouse (R. M. Hoek et al., submitted), suggest that myeloid function can be controlled in a tissue-specific manner by the OX2-OX2R interaction.
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, United Kingdom.
authors.forename: G J
authors.initials: GJ
authors.lastname: Wright
authors.forename: M J
authors.initials: MJ
authors.lastname: Puklavec
authors.forename: A C
authors.initials: AC
authors.lastname: Willis
authors.forename: R M
authors.initials: RM
authors.lastname: Hoek
authors.forename: J D
authors.initials: JD
authors.lastname: Sedgwick
authors.forename: M H
authors.initials: MH
authors.lastname: Brown
authors.forename: A N
authors.initials: AN
authors.lastname: Barclay
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D007136:Immunoglobulins / Q000502:physiology; D019891:Leukopoiesis / Q000502:physiology*; D008214:Lymphocytes / Q000502:physiology; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D008969:Molecular Sequence Data; D009474:Neurons / Q000502:physiology; D051381:Rats; D011971:Receptors, Immunologic / Q000502:physiology*; D016415:Sequence Alignment; D015398:Signal Transduction / Q000502:physiology
chemical_list: D007136:Immunoglobulins; D011971:Receptors, Immunologic