title: Anti-toxoplasma activities of 24 quinolones and fluoroquinolones in vitro: prediction of activity by molecular topology and virtual computational techniques.
abstract: The apicoplast, a plastid-like organelle of Toxoplasma gondii, is thought to be a unique drug target for quinolones. In this study, we assessed the in vitro activity of quinolones against T. gondii and developed new quantitative structure-activity relationship models able to predict this activity. The anti-Toxoplasma activities of 24 quinolones were examined by means of linear discriminant analysis (LDA) using topological indices as structural descriptors. In parallel, in vitro 50% inhibitory concentrations (IC(50)s) were determined in tissue culture. A multilinear regression (MLR) analysis was then performed to establish a model capable of classifying quinolones by in vitro activity. LDA and MLR analysis were applied to virtual structures to identify the influence of each atom or substituent of the quinolone ring on anti-Toxoplasma activity. LDA predicted that 20 of the 24 quinolones would be active against T. gondii. This was confirmed in vitro for most of the quinolones. Trovafloxacin, grepafloxacin, gatifloxacin, and moxifloxacin were the quinolones most potent against T. gondii, with IC(50)s of 0.4, 2.4, 4.1, and 5.1 mg/liter, respectively. Using MLR analysis, a good correlation was found between measured and predicted IC(50)s (r(2) = 0.87, cross-validation r(2) = 0.74). MLR analysis showed that the carboxylic group at position C-3 of the quinolone ring was not essential for anti-Toxoplasma activity. In contrast, activity was totally dependent on the presence of a fluorine at position C-6 and was enhanced by the presence of a methyl group at C-5 or an azabicyclohexane at C-7. A nucleophilic substituent at C-8 was essential for the activity of gatifloxacin and moxifloxacin.
authors.affiliation: Laboratoire de Parasitologie-Mycologie, Faculté de Médecine Lariboisière Saint-Louis, Université Paris 7, 75006 Paris, France.
authors.forename: R
authors.initials: R
authors.lastname: Gozalbes
authors.forename: M
authors.initials: M
authors.lastname: Brun-Pascaud
authors.forename: R
authors.initials: R
authors.lastname: Garcia-Domenech
authors.forename: J
authors.initials: J
authors.lastname: Galvez
authors.forename: P M
authors.initials: PM
authors.lastname: Girard
authors.forename: J P
authors.initials: JP
authors.lastname: Doucet
authors.forename: F
authors.initials: F
authors.lastname: Derouin
mesh_terms: D042462:4-Quinolones; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D003198:Computer Simulation; D024841:Fluoroquinolones; D008954:Models, Biological; D008968:Molecular Conformation; D011237:Predictive Value of Tests; D012044:Regression Analysis; D013329:Structure-Activity Relationship; D014122:Toxoplasma / Q000737:chemistry / Q000187:drug effects*
chemical_list: D042462:4-Quinolones; D000890:Anti-Infective Agents; D024841:Fluoroquinolones