Phosphorylation of neurofilament-L during LTD.
We recently reported that CaMKII-dependent phosphorylation of the neurofilament-L (NF-L) head domain was induced in the apical dendrites during long-term potentiation. Long-term depression (LTD) is another cellular model for neuronal plasticity. In the present study, we examined the phosphorylation of NF-L during hippocampal LTD using a series of phospho-specific antibodies against the NF-L head domain. During LTD, these antibodies visualized NF-L phosphorylation at Ser57 in the apical dendrites of the hippocampal pyramidal neurons. The assembly and disassembly of NF-L filaments are regulated by phosphorylation of its head domain. Thus, our results indicated that NF-L phosphorylation might be associated with alterations of the neuronal structure during LTD.
Hashimoto
R
R
Department of Clinical Neuroscience, Psychiatry, Osaka University Graduate School of Medicine, Japan.
Nakamura
Y
Y
Komai
S
S
Kashiwagi
Y
Y
Matsumoto
N
N
Shiosaka
S
S
Takeda
M
M
D000818:Animals; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism; D003712:Dendrites / Q000378:metabolism; D004594:Electrophysiology; D006624:Hippocampus / Q000502:physiology; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D017774:Long-Term Potentiation / Q000502:physiology*; D008297:Male; D051379:Mice; D016900:Neurofilament Proteins / Q000378:metabolism*; D010766:Phosphorylation; D017966:Pyramidal Cells / Q000378:metabolism; D013194:Staining and Labeling
D016900:Neurofilament Proteins; C044882:neurofilament protein L; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases