Superficially porous silica microspheres for fast high-performance liquid chromatography of macromolecules.
Very fast reversed-phase separations of biomacromolecules are performed using columns made with superficially porous silica microsphere column packings ("Poroshell"). These column packings consist of ultra-pure "biofriendly' silica microspheres composed of solid cores and thin outer shells with uniform pores. The excellent kinetic properties of these new column packings allow stable, high-resolution gradient chromatography of polypeptides, proteins, nucleic acids, DNA fragments, etc. in a fraction of the time required for conventional separations. Contrasted with <2-microm non-porous particles, Poroshell packings can be used optimally with existing equipment and greater sample loading capacities, while retaining kinetic (and separation speed) advantages over conventional totally porous particles.
Kirkland
J J
JJ
Agilent Technologies, Inc., Little Falls Chemical Analysis Group, Newport Site, DE 19804, USA.
Truszkowski
F A
FA
Dilks
C H
CH
Engel
G S
GS
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008863:Microspheres; D009696:Nucleic Acids / Q000032:analysis*; D010316:Particle Size; D011506:Proteins / Q000032:analysis*; D012822:Silicon Dioxide / Q000737:chemistry*
D009696:Nucleic Acids; D011506:Proteins; D012822:Silicon Dioxide