Extracellular superoxide dismutase in tissues from obese (ob/ob) mice.
We have examined the protein content and gene expression of three superoxide dismutase (SOD) isoenzymes in eight tissues from obese ob/ob mice, particularly placing the focus on extracellular-SOD (EC-SOD) in the white adipose tissue (WAT). Obesity significantly increased EC-SOD level in liver, kidney, testis, gastrocnemius muscle, WAT, brown adipose tissue (BAT), and plasma, but significantly decreased the isoenzyme level in lung. Tumor necrosis factor-alpha and interleukin-1beta contents in WAT were significantly higher in obese mice than in lean control mice. Immunohistochemically, both WAT and BAT from obese mice could be stained deeply with anti-mouse EC-SOD antibody compared with those from lean mice. Each primary culture per se almost time-dependently enhanced EC-SOD production, and overtly expressed its mRNA. The loss of heparin-binding affinity of EC-SOD type C with high affinity for heparin occurred in kidney of obese mice. These results suggest that the physiological importance of this SOD isoenzyme in WAT may be a compensatory adaptation to oxidative stress.
Nakao
C
C
Research Center of Health, Physical Fitness and Sports, Nagoya University, Aichi, Japan.
Ookawara
T
T
Sato
Y
Y
Kizaki
T
T
Imazeki
N
N
Matsubara
O
O
Haga
S
S
Suzuki
K
K
Taniguchi
N
N
Ohno
H
H
D000273:Adipose Tissue / Q000201:enzymology; D002001:Adipose Tissue, Brown / Q000201:enzymology; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D005110:Extracellular Space / Q000201:enzymology*; D006493:Heparin / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D018482:Muscle, Skeletal / Q000201:enzymology; D009765:Obesity / Q000201:enzymology*; D012333:RNA, Messenger / Q000032:analysis; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*; D013737:Testis / Q000201:enzymology
D001786:Blood Glucose; D016207:Cytokines; D007527:Isoenzymes; D012333:RNA, Messenger; D006493:Heparin; D013482:Superoxide Dismutase