title: Comparison of abilities of Salmonella enterica serovar typhimurium aroA aroD and aroA htrA mutants to act as live vectors.
abstract: We compared the ability of Salmonella enterica serovar Typhimurium SL1344 aroA aroD (BRD509) and aroA htrA (BRD807) mutants to act as live vectors for delivery of fragment C of tetanus toxin (FrgC). FrgC was expressed in these strains from either pTETnir15 or pTEThtrA1. BRD509FrgC(+) strains elicited approximately 2-log-higher serum anti-FrgC antibody titers than BRD807FrgC(+) strains. All mice immunized with BRD807pTEThtrA1, BRD509pTEThtrA1, and BRD509pTETnir15 (but not BRD807pTETnir15) were protected against tetanus.
authors.affiliation: Department of Veterinary Pathology, Glasgow University Veterinary School, Glasgow G61 1QH, United Kingdom. M.Roberts@vet.gla.ac.uk
authors.forename: M
authors.initials: M
authors.lastname: Roberts
authors.forename: S
authors.initials: S
authors.lastname: Chatfield
authors.forename: D
authors.initials: D
authors.lastname: Pickard
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: A
authors.initials: A
authors.lastname: Bacon
mesh_terms: D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase; D019883:Alkyl and Aryl Transferases / Q000235:genetics*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001428:Bacterial Vaccines / Q000276:immunology; D005822:Genetic Vectors; D006360:Heat-Shock Proteins; D006836:Hydro-Lyases / Q000235:genetics*; D007114:Immunization; D051379:Mice; D009154:Mutation; D010446:Peptide Fragments / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D033903:Periplasmic Proteins; D012481:Salmonella Infections, Animal / Q000517:prevention & control; D012486:Salmonella typhimurium / Q000235:genetics* / Q000276:immunology; D012697:Serine Endopeptidases / Q000235:genetics*; D013742:Tetanus / Q000517:prevention & control; D013744:Tetanus Toxin / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D014613:Vaccines, Attenuated
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D006360:Heat-Shock Proteins; D010446:Peptide Fragments; D033903:Periplasmic Proteins; D013744:Tetanus Toxin; D014613:Vaccines, Attenuated; C040635:tetanus toxin fragment C; D019883:Alkyl and Aryl Transferases; D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase; C061682:DegP protease; D012697:Serine Endopeptidases; D006836:Hydro-Lyases; C020000:3-dehydroquinate dehydratase