Mobilization of NK cells by exercise: downmodulation of adhesion molecules on NK cells by catecholamines.
The change of plasma catecholamine concentration correlates with the change of natural killer (NK) activity and NK cell number in peripheral blood mononuclear cells (PBMC) during and after moderate exercise. We studied the causal relation between exercise-induced catecholamine and expression of adhesion molecules on NK cells during and after exercise. The expression of CD44 and CD18 on CD3(-)CD56(+) NK cells was significantly reduced during exercise (P < 0.01). When PBMC were stimulated with 10(-8)M norepinephrine in vitro, the expression of these adhesion molecules on CD3(-)CD56(+) NK cells was downmodulated within 30 min. The binding capacity of NK cells to a CD44 ligand, hyaluronate, was reduced by the stimulation with norepinephrine (P < 0.01). The intravenous injection of norepinephrine in mice decreased the expression of CD44 and CD18 on CD3(-)NK1.1(+) cells (P < 0.01) and increased the number of CD3(-)NK1.1(+) cells in PBMC (P < 0.01). These findings suggest that exercise-induced catecholamines modulate the expression of adhesion molecules on NK cells, resulting in the mobilization of NK cells into the circulation.
Nagao
F
F
Department of Immunology, Juntendo University School of Medicine, Tokyo 113-8421, Japan.
Suzui
M
M
Takeda
K
K
Yagita
H
H
Okumura
K
K
D000328:Adult; D000818:Animals; D015703:Antigens, CD / Q000097:blood*; D018821:CD18 Antigens / Q000097:blood; D015815:Cell Adhesion Molecules / Q000097:blood*; D015232:Dinoprostone / Q000097:blood; D004298:Dopamine / Q000097:blood; D004837:Epinephrine / Q000097:blood; D015444:Exercise / Q000502:physiology; D005080:Exercise Test; D006801:Humans; D018960:Hyaluronan Receptors / Q000097:blood; D006854:Hydrocortisone / Q000097:blood; D007275:Injections, Intravenous; D039441:Integrin alpha4; D020014:K562 Cells; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009638:Norepinephrine / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D005082:Physical Exertion / Q000502:physiology*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000097:blood; D001615:beta-Endorphin / Q000097:blood
D015703:Antigens, CD; D018821:CD18 Antigens; D015815:Cell Adhesion Molecules; D018960:Hyaluronan Receptors; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D039441:Integrin alpha4; D001615:beta-Endorphin; D015232:Dinoprostone; D004298:Dopamine; D006854:Hydrocortisone; D009638:Norepinephrine; D004837:Epinephrine