Alcohol-induced conformational transitions in ervatamin C. An alpha-helix to beta-sheet switchover.
Alcohol-induced conformational transitions of erv C, a highly stable cysteine protease, were followed by CD, fluorescence, and activity. At acidic pH, the addition of different alcohols caused two types of conformational transitions. Increasing the concentration of nonfluorinated alkyl alcohols induced a conformational switch from alpha-helix to beta-sheet. Under these conditions, the protein lost its proteolytic activity and tertiary structure. The switch was a sudden one, observed in 50% methanol, 45% ethanol, and 40% propanol. Under similar conditions of pH and concentration, however, glycerol and TFE enhanced the alpha-helicity of the protein. Methanol-induced denaturation was observed to occur in two stages; the first is the beta-sheet state stabilized at low alcohol concentrations, and the other is the beta-sheet state with enhanced ellipticity stabilized at high alcohol concentrations. This beta-sheet conformation can be attained from the native as well as 6 M GuHCl-denatured state by addition of methanol and exhibits properties different from the native or unfolded state. This state shows loss of tertiary structure and activity, enhanced nonnative secondary structure, noncooperative temperature unfolding, and higher stability toward denaturants as compared to the native state, which are characteristic of the molten globule-like state or O-state, and thus this state may be functioning as an intermediate in the folding pathway of erv C.
Sundd
M
M
Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
Kundu
S
S
Jagannadham
M V
MV
D000438:Alcohols / Q000737:chemistry*; D002942:Circular Dichroism; D003546:Cysteine Endopeptidases / Q000737:chemistry* / Q000302:isolation & purification; D004795:Enzyme Stability; D019791:Guanidine / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007840:Latex / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification; D011487:Protein Conformation; D011489:Protein Denaturation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D013050:Spectrometry, Fluorescence; D013696:Temperature
D000438:Alcohols; D007840:Latex; D010940:Plant Proteins; D003546:Cysteine Endopeptidases; C116225:ervatamin; D019791:Guanidine