[Studies on kinetics of somatic embryo suspension culture in Picea meyeri Rehd. et Wils].
Embryogenic calli were produced from mature zygotic embryos of Picea meyeri Rehd. et Wils. which were cultured on modified LP medium containing 30 g/L sucrose, 2 mg/L 2,4-D and 1 mg/L 6-BA. The growth rate of embryogenic calli could be improved by way of suspension culture in medium MS containing 20 g/L sucrose, 1 mg/L 2,4-D and 1 mg/L KT. The optimum values of initial cell density and shake rate of flask in suspension culture were 3.0% (fresh weight) and 150 r/min respectively. Several parameters which might be correlated with growth were determined over a 10 day period. Maximum growth in terms of fresh weight, dry weight and number of ESM occurred during 6-10 day. pH and conductivity attained the lowest point during 6-8 day. A lot of somatic embryos were obtained following transfer of the suspension cultures to medium MS containing 5 mg/L ABA. Mature somatic embryos grew into plantlets with well developed cotyledons after being cultured on 1/2 MS medium supplemented with 0.5% activated charcoal but without hormone.
Yang
J L
JL
Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing.
Gui
Y L
YL
Guo
Z C
ZC
D002454:Cell Differentiation; D003470:Culture Media; D009924:Organ Culture Techniques; D010944:Plants / Q000196:embryology*; D012038:Regeneration; D013535:Suspensions
D003470:Culture Media; D013535:Suspensions