Neuronal mechanisms contribute to corticotropin-releasing factor-induced anti-oedema effect in the rat hind paw.
The present study is designed to elucidate the involvement of neuronal mechanisms in corticotropin-releasing factor (CRF)-induced anti-oedema effects. Oedema was induced in the rat hind paw by subcutaneous injection of 3 nmol of serotonin (5-HT). A single dose of CRF (9.4, 37.5 or 75 pmol) was given either ipsilaterally or contralaterally 30 min before 5-HT injection and oedema formation was subsequently measured every 30 min for 5.5 h. Compared to saline pre-treatment CRF (37.5 pmol) reduced oedema formation for 3.5 h when given ipsilaterally, and at 1.5 h (9.33, 37.5 and 75 pmol) when injected contralaterally. Administration of CRF along with CRF receptor antagonist, alpha-helical CRF, abolished the anti-oedema effects of CRF. Sciatic nerve ligation on the injected side attenuated the ipsilateral CRF-induced anti-oedema effect when compared with saline pre-treatment and sham-operated rats. Ipsilateral pre-treatment with 37.5 pmol of CRF caused a reduction in hind paw temperature compared to treatment with saline. Results of the present study indicate that the nervous system contributes to CRF effects in 5-HT-induced oedema formation.
Veraksits
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A
Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden. Indre.Bileviciute@fyfa.ki.se
Bileviciute-Ljungar
I
I
Maaroos
J
J
Vasar
E
E
Lundeberg
T
T
D000818:Animals; D003346:Corticotropin-Releasing Hormone / Q000494:pharmacology; D003714:Denervation; D004487:Edema / Q000139:chemically induced / Q000473:pathology*; D006614:Hindlimb / Q000098:blood supply / Q000294:innervation; D008297:Male; D017933:Peripheral Nervous System; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve; D012701:Serotonin; D014661:Vasoconstriction
D012701:Serotonin; D003346:Corticotropin-Releasing Hormone