Distribution of thyrotropin releasing hormone receptor in rats: an immunohistochemical study.
Thyrotropin releasing hormone (TRH) receptor was identified immunohistochemically in the rat tissues using anti-peptide antiserum. Anti-TRH receptor antiserum was raised in New Zealand white rabbits immunized with a conjugate of synthetic TRH-receptor peptide (15-28) to bovine serum albumin. Immunohistochemical analysis was performed by the avidin-biotin complex method. TRH-receptor immunoreactivity was visualized in the central nervous system and anterior pituitary thus supporting previous investigations of TRH receptor distribution using in vitro autoradiographic ligand binding. Significant stain was detected in neural perikarya, axons and dendrites as well as in many cells of the retina, adrenal medulla, stomach mucosa, Auerbach's nervous branch and Mysner's nervous branch of the stomach, small intestine and colon. When using antiserum preincubated with synthetic TRH-receptor peptide (15-28) or rat anterior pituitary homogenate which contains TRH-receptor peptide, no significant stain of the anterior pituitary cells or neurons in the hypothalamus was detected. These findings suggest that TRH-receptor is widely distributed and that this method is valuable in studying the distribution of TRH-receptor in rats.
Mitsuma
T
The Four Department of Internal Medicine, Laboratory Medicine and First Department of Physiology, Aichi Medical University, Nagakute, Aichi, Aichi 480-11, Japan and Department of Internal Medicine Konanshowa Hospital, Konan Aichi, Japan.
Rhue
N
Sobue
G
Hirooka
Y
Kayama
M
Yokoi
Y
Adachi
K
Nogimori
T
Sakai
J
Sugie
I