title: [Proteolytic activity of the enzyme complex of the mammalian pancreas in comparison with pancreatin].
abstract: The proteolytic activity and thermal stability of the enzyme complex of cell suspension from pig and bovine pancreas glands was compared with those of pancreatin. The enzyme complex displayed the highest thermal stability and activity at 50 degrees C. The kinetic constants, energies of activation and inactivation of the enzyme complex, and pH optimum (7.0 +/- 0.1) at which this complex had the maximum proteolytic activity were determined. Pancreatin had a pH optimum of 8.0 +/- 0.1.
authors.affiliation: All-Russia Institute of Meat Industry, Moscow, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Berdutina
authors.forename: A D
authors.initials: AD
authors.lastname: Nekliudov
authors.forename: A I
authors.initials: AI
authors.lastname: Ivankin
authors.forename: B S
authors.initials: BS
authors.lastname: Karpo
authors.forename: S I
authors.initials: SI
authors.lastname: Mitaleva
mesh_terms: D000818:Animals; D002417:Cattle; D004789:Enzyme Activation; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D009097:Multienzyme Complexes / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010179:Pancreas / Q000201:enzymology*; D010194:Pancreatin / Q000378:metabolism*
chemical_list: D009097:Multienzyme Complexes; D010194:Pancreatin