The effect of acute insulin-like growth factor-II administration on glucose metabolism in the rat.
The in vivo effects of the insulin-like growth factor-II (IGF-II) on glucose metabolism is not yet well defined. To assess the acute effect of IGF-II administration on whole body glucose utilization and hepatic glucose production, we used the well-established euglycemic clamp technique and compared the effects in awake cannulated rats with those of insulin. Each animal underwent several 90-min euglycemic studies, alternating between IGF-II and insulin. Following IGF-II infusion, tissue glucose uptake was increased to 9.8 +/- 0.6 mg/kg/min (mean +/- SEM), which represented only 14% of the effect of insulin, despite the molar plasma concentration ratio of insulin: IGF-2 being 1:460. IGF-II and insulin infusion reduced hepatic glucose output by 49 and 75%, respectively. Thus, IGF-II, administered acutely, affects glucose homeostasis in a manner very similar to insulin, probably via the insulin receptors, although with significantly lower potency.
Burvin
R
R
Institute of Endocrinology, Diabetes and Metabolism, Rambam Medical Center and B. Rappaport Faculty of Medicine, Technion Haifa, Israel.
LeRoith
D
D
Harel
H
H
Zloczower
M
M
Marbach
M
M
Karnieli
E
E
D000818:Animals; D005947:Glucose / Q000378:metabolism*; D015309:Glucose Clamp Technique; D007003:Hypoglycemia / Q000139:chemically induced; D007335:Insulin-Like Growth Factor II / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D007335:Insulin-Like Growth Factor II; D005947:Glucose