title: Chemokine gene expression in astrocytes of Borna disease virus-infected rats and mice in the absence of inflammation.
abstract: Borna disease virus (BDV) causes CD8(+) T-cell-mediated meningoencephalitis in immunocompetent mice and rats, thus providing a valuable animal model for studying the mechanisms of virus-induced central nervous system (CNS) immunopathology. Chemokine-mediated leukocyte recruitment to the CNS is a crucial step in the development of neurological disease. We found increased mRNA levels of IP-10 and other chemokines in brains of adult rats following infection with BDV. The marked increase in chemokine gene expression at about day 8 postinfection seemed to immediately precede the inflammatory process. In brains of rats infected as newborns, in which inflammation was only mild and transient, sustained expression of IP-10 and RANTES genes was observed. In situ hybridization studies revealed that astrocytes were the major source of IP-10 mRNAs in brains of rats infected as newborns and as adults. In brains of infected mice lacking CD8(+) T cells (beta2m(0/0)), transcripts encoding IP-10 and RANTES were also observed. IP-10 transcripts were also present in a small number of scattered astrocytes of infected knockout mice lacking mature B and T cells as well as functional alpha/beta and gamma interferon receptors, indicating that BDV can induce chemokine synthesis in the absence of interferons and other B- or T-cell-derived cytokines. These data provide strong evidence that CNS-resident cells are involved in the early localized host immune response to infection with BDV and support the concept that chemokines are pivotal for the initiation of virus-induced CNS inflammation.
authors.affiliation: Abteilung Virologie, Institut für Medizinische Mikrobiologie und Hygiene, Universität Freiburg, D-79104 Freiburg.
authors.forename: C
authors.initials: C
authors.lastname: Sauder
authors.forename: W
authors.initials: W
authors.lastname: Hallensleben
authors.forename: A
authors.initials: A
authors.lastname: Pagenstecher
authors.forename: S
authors.initials: S
authors.lastname: Schneckenburger
authors.forename: L
authors.initials: L
authors.lastname: Biro
authors.forename: D
authors.initials: D
authors.lastname: Pertlik
authors.forename: J
authors.initials: J
authors.lastname: Hausmann
authors.forename: M
authors.initials: M
authors.lastname: Suter
authors.forename: P
authors.initials: P
authors.lastname: Staeheli
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000276:immunology* / Q000821:virology*; D001890:Borna Disease / Q000235:genetics* / Q000276:immunology*; D001891:Borna disease virus; D018925:Chemokines / Q000096:biosynthesis / Q000235:genetics*; D015967:Gene Expression Regulation, Viral; D007249:Inflammation; D008297:Male; D051379:Mice; D051381:Rats; D011917:Rats, Inbred Lew
chemical_list: D018925:Chemokines