In-house low-resolution X-ray crystallography.
It is demonstrated that standard in-house protein crystal X-ray diffraction apparatus can be used to measure very low resolution reflections with only a few modifications. The apparatus and modifications are described in detail and tested on two different macromolecular crystal samples: lysozyme and the 30S ribosomal subunit. Contrast-variation measurements on tetragonal hen egg-white lysozyme demonstrate the potential usefulness of the apparatus in providing accurate data for the determination of macromolecular envelopes. In contrast, the measurement of very low resolution diffraction from crystals of the 30S ribosome subunit illustrates how in-house facilities can provide data from small weakly diffracting crystals of a very large macromolecule.
Evans
G
G
MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, England. gwyndaf@mrc-lmb.cam.ac.uk
Roversi
P
P
Bricogne
G
G
D000818:Animals; D002645:Chickens; D018360:Crystallography, X-Ray / Q000295:instrumentation* / Q000379:methods*; D004867:Equipment Design; D009113:Muramidase / Q000737:chemistry*; D012270:Ribosomes / Q000648:ultrastructure*; D012680:Sensitivity and Specificity
D009113:Muramidase