Multiple promoters direct the tissue-specific expression of rat mitochondrial glycerol-3-phosphate dehydrogenase.
The mitochondrial FAD-dependent glycerol-3-phosphate dehydrogenase (mGPDH) is an essential component of the glycerol phosphate shuttle which transfers reduction equivalents from the cytoplasm into the mitochondria. We analyzed the distribution of different exon 1-containing transcripts by RT-PCR in various tissues in vivo. Exon 1 a was predominantly expressed in brain, brown adipose tissue and pancreas, exon 1b was ubiquitously expressed, and exon 1c was exclusively expressed in testis. In transient transfection assays the ubiquitous promoter B showed a detectable activity, whereas promoters A and C were completely silent. A deletion mutational analysis located the basal promoter B activity to a 316 bp core sequence upstream of the transcription start site.
Weitzel
J M
JM
Institut für Medizinische Biochemie und Molekularbiologie, Universitäts-Krankenhaus Eppendorf, Hamburg, Germany.
Grott
S
S
Radtke
C
C
Kutz
S
S
Seitz
H J
HJ
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics*; D006801:Humans; D008928:Mitochondria / Q000201:enzymology*; D011401:Promoter Regions, Genetic; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D005993:Glycerolphosphate Dehydrogenase