title: Regulation of GIRK channel deactivation by Galpha(q) and Galpha(i/o) pathways.
abstract: G protein regulated inward rectifying potassium channels (GIRKs) are activated by G protein coupled receptors (GPCRs) via the G protein betagamma subunits. However, little is known about the effects of different GPCRs on the deactivation kinetics of transmitter-mediated GIRK currents. In the present study we investigated the influence of different GPCRs in the presence and absence of RGS proteins on the deactivation kinetics of GIRK channels by coexpressing the recombinant protein subunits in Xenopus oocytes. The stimulation of both G(i/o)- and G(q)-coupled pathways accelerated GIRK deactivation. GIRK currents deactivated faster upon stimulation of G(i/o)- and G(q)-coupled pathways by P(2)Y(2) receptors (P(2)Y(2)Rs) than upon activation of the G(i/o)-coupled pathway alone via muscarinic acetylcholine receptor M2 (M(2) mAChRs). This acceleration was found to be dependent on phospholipase C (PLC) and protein kinase C (PKC) activities and intracellular calcium. With the assumption that RGS2 has a higher affinity for Galpha(q) than Galpha(i/o), we demonstrated that the deactivation kinetics of GIRK channels can be differentially regulated by the relative amount of RGS proteins. These data indicate that transmitter-mediated deactivation of GIRK currents is modulated by crosstalk between G(i/o)- and G(q)-coupled pathways.
authors.affiliation: Department of Physiology II, University of Tuebingen, Ob dem Himmelreich 7, 72074, Tuebingen, Germany.
authors.forename: M D
authors.initials: MD
authors.lastname: Mark
authors.forename: J P
authors.initials: JP
authors.lastname: Ruppersberg
authors.forename: S
authors.initials: S
authors.lastname: Herlitze
mesh_terms: D000818:Animals; D002118:Calcium / Q000502:physiology; D018118:Chloride Channels / Q000187:drug effects / Q000378:metabolism; D003001:Cloning, Molecular; D051676:G Protein-Coupled Inwardly-Rectifying Potassium Channels; D043802:GTP-Binding Protein alpha Subunits, Gq-G11; D020962:Heterotrimeric GTP-Binding Proteins / Q000378:metabolism*; D006801:Humans; D026902:Potassium Channel Blockers; D015221:Potassium Channels / Q000378:metabolism*; D024661:Potassium Channels, Inwardly Rectifying; D011493:Protein Kinase C / Q000378:metabolism; D043585:Receptor, Muscarinic M2; D011976:Receptors, Muscarinic / Q000378:metabolism; D018048:Receptors, Purinergic P2 / Q000378:metabolism; D058473:Receptors, Purinergic P2Y2; D010738:Type C Phospholipases / Q000378:metabolism; D014982:Xenopus laevis
chemical_list: D018118:Chloride Channels; D051676:G Protein-Coupled Inwardly-Rectifying Potassium Channels; C546385:P2RY2 protein, human; D026902:Potassium Channel Blockers; D015221:Potassium Channels; D024661:Potassium Channels, Inwardly Rectifying; D043585:Receptor, Muscarinic M2; D011976:Receptors, Muscarinic; D018048:Receptors, Purinergic P2; D058473:Receptors, Purinergic P2Y2; D011493:Protein Kinase C; D010738:Type C Phospholipases; D043802:GTP-Binding Protein alpha Subunits, Gq-G11; D020962:Heterotrimeric GTP-Binding Proteins; D002118:Calcium