IgE production after antigen-specific and cognate activation of HLA-DPw4-restricted T-cell clones, by 78% of randomly selected B-cell donors.
The frequency of expression of the MHC class II antigen, HLA-DPw4, in the caucasoid population is approximately 78%, and is unmatched by phenotypic frequencies of other HLA class II molecules. Here we describe three human Der-P1-specific T-cell clones (TCC), restricted by the HLA-DPw4-variant HLA-DPB1*0401, of which two TCC also responded to antigen, presented on HLA-DPB1*0402. Thus, randomly selected caucasoid donors present a 78% chance for a correct match with these HLA-DPw4-restricted TCC. This allows comparative in vitro antigen presentation studies with various antigen presenting cells (APC) from different (healthy or diseased) donors without the variable influence of responding T cells. It was subsequently demonstrated that the TCC can be used to study antigen-induced IgE production in randomly selected primary B cells. Cognate HLA-DPw4-restricted antigen presentation caused enhanced immunoglobulin production of IgE, IgG1, IgA and IgM, of which only IgE induction was reversed by addition of anti-IL-4 antibodies.
Baselmans
P J
PJ
Novartis Research Institute, Department of Immunology, Vienna, Austria. pjbaselmans@yahoo.com
Pöllabauer
E
E
van Reijsen
F C
FC
Heystek
H C
HC
Hren
A
A
Stumptner
P
P
Tilanus
M G
MG
Vooijs
W C
WC
Mudde
G C
GC
D000938:Antigen-Presenting Cells / Q000276:immunology; D039741:Antigens, Dermatophagoides; D001402:B-Lymphocytes / Q000276:immunology*; D002455:Cell Division; D002461:Cell Line, Transformed; D002999:Clone Cells; D006023:Glycoproteins / Q000276:immunology; D006682:HLA-DP Antigens / Q000276:immunology*; D059868:HLA-DP beta-Chains; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis*; D014066:Palatine Tonsil / Q000166:cytology; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014019:Tissue Donors
D039741:Antigens, Dermatophagoides; D006023:Glycoproteins; D006682:HLA-DP Antigens; D059868:HLA-DP beta-Chains; C071170:HLA-DPB1 antigen; C056284:HLA-DPw4 antigen; D011948:Receptors, Antigen, T-Cell; D007073:Immunoglobulin E