In vitro antimicrobial properties of recombinant ASABF, an antimicrobial peptide isolated from the nematode Ascaris suum.
ASABF is a CSalphabeta-type antimicrobial peptide that contains four intramolecular disulfide bridges (Y. Kato and S. Komatsu, J. Biol. Chem. 271:30493-30498, 1996). In the present study, a recombinant ASABF was produced by using a yeast expression system, and its antimicrobial activity was characterized in detail. The recombinant ASABF was active against all gram-positive bacteria tested (7 of 7; minimum bactericidal concentration [MBC], 0.03 to 1 microg/ml) except Leuconostoc mesenteroides, some gram-negative bacteria (8 of 14; MBC, >0.5 microg/ml), and some yeasts (3 of 9; MBC >3 microg/ml). Slight hemolytic activity (4.2% at 100 microg/ml) against human erythrocytes was observed only under low-ionic-strength conditions. Less than 1 min of contact was enough to kill Staphylococcus aureus ATCC 6538P. The bactericidal activity against S. aureus was inhibited by salts.
Zhang
H
H
National Institute of Sericultural and Entomological Science, Tsukuba, Japan.
Yoshida
S
S
Aizawa
T
T
Murakami
R
R
Suzuki
M
M
Koganezawa
N
N
Matsuura
A
A
Miyazawa
M
M
Kawano
K
K
Nitta
K
K
Kato
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000494:pharmacology*; D017165:Ascaris suum / Q000737:chemistry / Q000378:metabolism*; D001419:Bacteria / Q000187:drug effects*; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D005822:Genetic Vectors; D015801:Helminth Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000494:pharmacology; D015003:Yeasts / Q000187:drug effects
C102532:ASABF protein, Ascaris suum; D000900:Anti-Bacterial Agents; D015801:Helminth Proteins; D011994:Recombinant Proteins