Optical mapping of BAC clones from the human Y chromosome DAZ locus.
The accurate mapping of clones derived from genomic regions containing complex arrangements of repeated elements presents special problems for DNA sequencers. Recent advances in the automation of optical mapping have enabled us to map a set of 16 BAC clones derived from the DAZ locus of the human Y chromosome long arm, a locus in which the entire DAZ gene as well as subsections within the gene copies have been duplicated. High-resolution optical mapping employing seven enzymes places these clones into two contigs representing four distinct copies of the DAZ gene and highlights a number of differences between individual copies of DAZ.
Giacalone
J
J
W.M. Keck Laboratory for Biomolecular Imaging, Department of Chemistry, New York University, New York, New York 10003, USA.
Delobette
S
S
Gibaja
V
V
Ni
L
L
Skiadas
Y
Y
Qi
R
R
Edington
J
J
Lai
Z
Z
Gebauer
D
D
Zhao
H
H
Anantharaman
T
T
Mishra
B
B
Brown
L G
LG
Saxena
R
R
Page
D C
DC
Schwartz
D C
DC
D002874:Chromosome Mapping / Q000295:instrumentation / Q000379:methods*; D002876:Chromosomes, Bacterial / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods; D020451:Contig Mapping; D000075364:Deleted in Azoospermia 1 Protein; D015252:Deoxyribonucleases, Type II Site-Specific / Q000032:analysis; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D016254:Mutagenesis, Insertional; D016601:RNA-Binding Proteins / Q000032:analysis / Q000235:genetics*; D015183:Restriction Mapping; D014998:Y Chromosome / Q000235:genetics*
C095668:DAZ1 protein, human; D000075364:Deleted in Azoospermia 1 Protein; D005819:Genetic Markers; D016601:RNA-Binding Proteins; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases