Quantitative determination of salidroside and tyrosol from the underground part of Rhodiola rosea by high performance liquid chromatography.
A reversed-phase high performance liquid chromatographic method was developed to determine salidroside and tyrosol simultaneously in the Rhodiola rosea. The optimum condition was Nova-Pak C18 as stationary phase, 6.5% methanol in water as mobile phase and detection at UV 225 nm. The identification was carried out by comparing the retention time and IC/MS spectrum of the relevant peaks with those of isolated standards. The contents of salidroside and tyrosol in the samples gathered from various area in China were ranged over 1.3-11.1 mg/g and 0.3-2.2 mg/g, respectively.
Linh
P T
PT
College of Pharmacy, Chungnam National University, Taejon, Korea.
Kim
Y H
YH
Hong
S P
SP
Jian
J J
JJ
Kang
J S
JS
D002851:Chromatography, High Pressure Liquid; D005960:Glucosides / Q000032:analysis*; D010636:Phenols; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000032:analysis; D010946:Plants, Medicinal / Q000737:chemistry*
D005960:Glucosides; D010636:Phenols; C011867:4-hydroxyphenylethanol; C009172:rhodioloside; D010626:Phenylethyl Alcohol