title: Genotyping of Pneumocystis carinii f. sp. hominis isolates in Japan based on nucleotide sequence variations in internal transcribed spacer regions of rRNA genes.
abstract: Genotyping of Pneumocystis carinii (Pc) isolated from 24 bronchoalveolar lavage (BAL) fluid specimens in Japan was examined based on nucleotide sequence variations in internal transcribed spacer regions 1 and 2 (ITS1 and ITS2, respectively) of rRNA genes. We found 11 ITS1 genotypes including 2 novel ones and 11 ITS2 genotypes including 3 new ones. Combining the ITS1 and ITS2 genotypes resulted in 30 ITS genotypes, of which 10 are newly described in this report. Two or more genotypes in ITS regions in a specimen were observed in 16 of 24 patients. Our results will be of help for the epidemiological investigation of Pc infection.
authors.affiliation: Department of Infectious Diseases, Institute of Medical Science, The University of Tokyo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Hosoya
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
authors.forename: M
authors.initials: M
authors.lastname: Wada
authors.forename: T
authors.initials: T
authors.lastname: Endo
authors.forename: T
authors.initials: T
authors.lastname: Nakamura
authors.forename: H
authors.initials: H
authors.lastname: Sakashita
authors.forename: K
authors.initials: K
authors.lastname: Kimura
authors.forename: K
authors.initials: K
authors.lastname: Ohnishi
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: T
authors.initials: T
authors.lastname: Mizuochi
authors.forename: A
authors.initials: A
authors.lastname: Iwamoto
mesh_terms: D000328:Adult; D001483:Base Sequence; D005260:Female; D005800:Genes, Fungal; D016681:Genome, Fungal; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D011010:Pneumocystis / Q000235:genetics* / Q000302:isolation & purification; D012335:RNA, Ribosomal / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D012335:RNA, Ribosomal