Identification of cyprinol and cyprinol sulfate from grass carp bile and their toxic effects in rats.
To elucidate the responsible toxic components of grass carp bile, the bile salt 5 alpha-cyprinol sulfate and its desalted form 5 alpha-cyprinol from grass carp bile were purified and identified by analyses of infrared spectrum, (1)H-, (13)C-nuclear magnetic resonance spectra and mass spectrum. The toxicity of grass carp bile powder, butanol extract of grass carp bile powder, 5 alpha-cyprinol and 5 alpha-cyprinol sulfate in rats were further determined. The kidney and liver functions were significantly affected by grass carp bile powder, butanol extract and 5 alpha-cyprinol sulfate. However, 5 alpha-cyprinol also significantly affected the kidney function, but the toxic effect was less.
Hwang
D F
DF
Department of Food Science, National Taiwan Ocean University, Keelung. dfhwang@mail.ntou.edu.tw
Yeh
Y H
YH
Lai
Y S
YS
Deng
J F
JF
D000284:Administration, Oral; D000818:Animals; D001647:Bile Acids and Salts / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity*; D002347:Carps; D002777:Cholestanols / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity*; D007668:Kidney / Q000187:drug effects; D008099:Liver / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D001647:Bile Acids and Salts; D002777:Cholestanols; C023378:anhydrocyprinol