title: Synergy between enzymes from Aspergillus involved in the degradation of plant cell wall polysaccharides.
abstract: Synergy in the degradation of two plant cell wall polysaccharides, water insoluble pentosan from wheat flour (an arabinoxylan) and sugar beet pectin, was studied using several main-chain cleaving and accessory enzymes. Synergy was observed between most enzymes tested, although not always to the same extent. Degradation of the xylan backbone by endo-xylanase and beta-xylosidase was influenced most strongly by the action of alpha-L-arabinofuranosidase and arabinoxylan arabinofuranohydrolase resulting in a 2.5-fold and twofold increase in release of xylose, respectively. Ferulic acid release by feruloyl esterase A and 4-O-methyl glucuronic acid release by alpha-glucuronidase depended largely on the degradation of the xylan backbone by endo-xylanase but were also influenced by other enzymes. Degradation of the backbone of the pectin hairy regions resulted in a twofold increase in the release of galactose by beta-galactosidase and endo-galactanase but did not significantly influence the arabinose release by arabinofuranosidase and endo-arabinase. Ferulic acid release from sugar beet pectin by feruloyl esterase A was affected most strongly by the presence of other accessory enzymes.
authors.affiliation: Molecular Genetics of Industrial Microorganisms, Wageningen University, The Netherlands.
authors.forename: R P
authors.initials: RP
authors.lastname: de Vries
authors.forename: H C
authors.initials: HC
authors.lastname: Kester
authors.forename: C H
authors.initials: CH
authors.lastname: Poulsen
authors.forename: J A
authors.initials: JA
authors.lastname: Benen
authors.forename: J
authors.initials: J
authors.lastname: Visser
mesh_terms: D001089:Arabinose / Q000378:metabolism; D001230:Aspergillus / Q000201:enzymology*; D002473:Cell Wall / Q000737:chemistry*; D019663:Chenopodiaceae / Q000737:chemistry / Q000648:ultrastructure; D003373:Coumaric Acids / Q000378:metabolism; D004357:Drug Synergism; D006867:Hydrolases / Q000378:metabolism*; D010368:Pectins / Q000032:analysis / Q000378:metabolism; D010944:Plants / Q000737:chemistry / Q000648:ultrastructure; D011134:Polysaccharides / Q000378:metabolism*; D014908:Triticum / Q000737:chemistry / Q000648:ultrastructure; D014990:Xylans / Q000032:analysis / Q000378:metabolism*
chemical_list: D003373:Coumaric Acids; D011134:Polysaccharides; D014990:Xylans; D010368:Pectins; C004999:ferulic acid; D001089:Arabinose; D006867:Hydrolases