title: Beta-keto-ester chemistry and ketolides. Synthesis and antibacterial activity of 2-halogeno, 2-methyl and 2,3 enol-ether ketolides.
abstract: The effect of 2,3 modifications on the antibacterial activity of ketolides was evaluated by introducing substituents in position 2 and converting the C-1, C-2, C-3 beta-keto-ester into stable 2,3 enol-ether or 2,3 anhydro derivatives. Introduction of a fluorine in C-2 is beneficial with regard to the overall antibacterial spectrum whereas the enol-ether and 2,3 unsaturated compounds, as well as the bulky gem dimethyl or 2-chloro derivatives, are less active particularly against erythromycin resistant strains. A 2-fluoro ketolide derivative demonstrates good antibacterial activity and in vivo efficacy against multi-resistant Streptococcus pneumoniae. Compared to azithromycin against Haemophilus influenzae, this compound is equivalent in vitro and slightly more active in vivo. These results demonstrate that within the ketolide class, to retain good antibacterial activity, position 2 needs to remain tetrahedral and tolerates only very small substituents such as fluorine.
authors.affiliation: Medicinal Chemistry, Aventis Pharma, Romainville, France. alexis.denis@aventis.com
authors.forename: A
authors.initials: A
authors.lastname: Denis
authors.forename: F
authors.initials: F
authors.lastname: Bretin
authors.forename: C
authors.initials: C
authors.lastname: Fromentin
authors.forename: A
authors.initials: A
authors.lastname: Bonnet
authors.forename: G
authors.initials: G
authors.lastname: Piltan
authors.forename: A
authors.initials: A
authors.lastname: Bonnefoy
authors.forename: C
authors.initials: C
authors.lastname: Agouridas
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects*; D001424:Bacterial Infections / Q000188:drug therapy; D018942:Macrolides; D051379:Mice; D013329:Structure-Activity Relationship
chemical_list: D000900:Anti-Bacterial Agents; D018942:Macrolides