title: Two efflux systems expressed simultaneously in multidrug-resistant Pseudomonas aeruginosa.
abstract: Simultaneous overexpression of MexAB-OprM and MexEF-OprN was demonstrated for a multiply antibiotic-resistant clinical isolate of Pseudomonas aeruginosa (G49). G49 also had decreased expression of OprF. No mutations in mexR or its upstream promoter region, mexT, oprM, oprF, or sigX were revealed, suggesting regulation by a hitherto undescribed locus.
authors.affiliation: Antimicrobial Agents Research Group, Division of Immunity and Infection, University of Birmingham, Birmingham B15 2TT, United Kingdom.
authors.forename: L
authors.initials: L
authors.lastname: Pumbwe
authors.forename: L J
authors.initials: LJ
authors.lastname: Piddock
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics*; D015153:Blotting, Western; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics*; D018432:Drug Resistance, Multiple; D004591:Electrophoresis, Polyacrylamide Gel; D026901:Membrane Transport Proteins; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D018272:Porins / Q000096:biosynthesis / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001425:Bacterial Outer Membrane Proteins; D002352:Carrier Proteins; D026901:Membrane Transport Proteins; C402263:OprM protein, Pseudomonas aeruginosa; C105708:OprN protein, Pseudomonas aeruginosa; D018272:Porins