title: Identification of lipoprotein homologues of pneumococcal PsaA in the equine pathogens Streptococcus equi and Streptococcus zooepidemicus.
abstract: Streptococcus equi and Streptococcus zooepidemicus are major etiological agents of upper and lower airway disease in horses. Despite the considerable animal suffering and economic burden associated with these diseases, the factors that contribute to the virulence of these equine pathogens have not been extensively investigated. Here we demonstrate the presence of a homologue of the Streptococcus pneumoniae PsaA protein in both of these equine pathogens. Inhibition of signal peptide processing by the antibiotic globomycin confirmed the lipoprotein nature of the mature proteins, and surface exposure was confirmed by their release from intact cells by mild trypsinolysis.
authors.affiliation: School of Sciences, University of Sunderland, Sunderland, United Kingdom. dean.harrington@sunderland.ac.uk
authors.forename: D J
authors.initials: DJ
authors.lastname: Harrington
authors.forename: J S
authors.initials: JS
authors.lastname: Greated
authors.forename: N
authors.initials: N
authors.lastname: Chanter
authors.forename: I C
authors.initials: IC
authors.lastname: Sutcliffe
mesh_terms: D018829:Adhesins, Bacterial; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006734:Horse Diseases / Q000382:microbiology*; D006736:Horses; D008074:Lipoproteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013290:Streptococcal Infections / Q000382:microbiology / Q000662:veterinary; D018502:Streptococcus equi / Q000235:genetics* / Q000472:pathogenicity; D014774:Virulence
chemical_list: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D002352:Carrier Proteins; D008074:Lipoproteins; D026901:Membrane Transport Proteins; C085000:PsaA protein, Streptococcus