Expression characteristics and relevance of sodium glucose cotransporter-1 in mammalian renal tubulogenesis.
Expression and role of sodium glucose cotransporter (SGLT-1) in tubulogenesis were investigated during renal development. A mouse SGLT-1 cDNA was cloned, and it had substantial homology with human and rat forms. Four mRNA transcripts were detected, which differed in size from other species. SGLT-1 transcripts were detected at day 13 of gestation, and their expression increased during later stages extending into the postnatal period. A high mRNA and protein expression of SGLT-1 was seen in tubular segments of the inner cortex and outer medulla at day 16, and it was developmentally regulated. Treatment with SGLT-1 antisense selectively decreased the population of tubules in the metanephric explants. Expression of glomerular mRNA and WGA binding were unchanged. SGLT-1 activity, as measured by [(14)C]methyl-alpha-D-glucopyranoside uptake, increased during gestation in the tubular segments where it is expressed. Glucose uptake was inhibited by the treatment with SGLT-1 antisense and D-galactose. The data suggest that SGLT-1 exhibits a restricted spatiotemporal expression with functional activity confined to the corresponding tubular segments, and it selectively maintains renal tubulogenesis during development.
Yang
Q
Q
Departments of Pathology and Medicine, Northwestern University Medical School, Chicago, Illinois 60611, USA.
Tian
Y
Y
Wada
J
J
Kashihara
N
N
Wallner
E
E
Peterson
D
D
Kanwar
Y S
YS
D000595:Amino Acid Sequence / Q000235:genetics; D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004622:Embryo, Mammalian / Q000378:metabolism / Q000502:physiology; D005314:Embryonic and Fetal Development / Q000502:physiology; D015870:Gene Expression / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007684:Kidney Tubules / Q000196:embryology*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017386:Sequence Homology, Amino Acid; D051269:Sodium-Glucose Transporter 1
D018076:DNA, Complementary; D008562:Membrane Glycoproteins; D009004:Monosaccharide Transport Proteins; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; C495674:SLC5A1 protein, human; C495675:Slc5a1 protein, mouse; C495676:Slc5a1 protein, rat; D051269:Sodium-Glucose Transporter 1; D005947:Glucose