Inhibitory effect of mast cell-dependent anaphylaxis by Gleditsia sinensis.
We investigated the effect of aqueous extract of Gleditsia sinensis thorns (Leguminosae) (GSAE) on the mast cell-dependent anaphylaxis. GSAE (0.005 to 1 g/kg) dose-dependently inhibited systemic anaphylaxis induced by compound 48/80 in rats. GSAE (0.1 and 1 g/kg) also significantly inhibited local anaphylaxis activated by anti-DNP IgE. When GSAE was pretreated at the same concentrations with systemic anaphylaxis, the plasma histamine levels were reduced in a dose-dependent manner. GSAE (0.001 to 1 mg/ml) dose-dependently inhibited the histamine release from rat peritoneal mast cells (RPMC) activated by compound 48/80 or anti-DNP IgE. The level of cyclic AMP in RPMC, When CSAE (1 mg/ml) was added, transiently and significantly increased about fourfold compared with that of basal cells. Moreover, GSAE (0.01 and 0.1 mg/ml) had a significant inhibitory effect on anti-DNP IgE-induced tumor necrosis factor-alpha production from RPMC. These results suggest a possible use of GSAE in managing mast cell-dependent anaphylaxis.
Shin
T Y
TY
College of Pharmacy, Woosuk University, Chonju, Chonbuk, Korea. tyshin@core.woosuk.ac.kr
Kim
D K
DK
D000707:Anaphylaxis / Q000517:prevention & control*; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000242:Cyclic AMP / Q000032:analysis; D006636:Histamine Release / Q000187:drug effects; D007723:Korea; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000502:physiology; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology; D010946:Plants, Medicinal; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D000888:Antibodies, Anti-Idiotypic; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; C026408:anti-IgE antibodies; D003189:p-Methoxy-N-methylphenethylamine; D000242:Cyclic AMP