[Methods for checking up screening tests for detection of shiga toxin producing Escherichia coli (STEC) using PCR].
Methods for making check ups of STEC-Screening-PCRs by using internal and external systems are given. A control-DNA with identical primer binding sites in relation to the target-DNA (stx-genes) was produced and by HPLC-chromatography quantified. It was used in an internal system. But we had to use too high concentrations of this DNA for making the check up in each sample. A false negative result could be shown in samples with a low stx content as a result of this coamplification process. Therefore we used an external system. A control-DNA with heterologous primer binding sites in relation to the stx-screening-PCR and the special primers were given in each vessel. The optimized system was used for making investigations of some faecal samples from cattle.
Gallien
P
P
Bundesinstitut für gesundheitlichen Verbraucherschutz und Veterinärmedizin in Dessau.
Much
C
C
Müller
M
M
Perlberg
K W
KW
Protz
D
D
D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000382:microbiology; D002851:Chromatography, High Pressure Liquid; D004768:Enterotoxins / Q000235:genetics; D004926:Escherichia coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000175:diagnosis / Q000662:veterinary*; D005243:Feces / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods; D022603:Shiga Toxins
D001427:Bacterial Toxins; D004768:Enterotoxins; D022603:Shiga Toxins