Preventive effect of vitamin E-containing liposome instillation on cataract progression in 12-month-old rats fed a 25% galactose diet.
The preventive effect of vitamin E (Vit. E)-containing liposome instillation on cataract progression was examined in 12-month-old Wistar rats fed a 25% galactose diet. Vit. E-containing liposomes prepared with dipalmitoylphosphatidylcholine and dioleoylphosphatidylcholine (7:3 w/w) were instilled into both eyes twice a day. Lenses of galactose-fed rats showed suture accentuation at 6 months of feeding and opacities in the cortex and nuclei at 8 months. Two months of Vit. E-containing liposome instillation, starting at 6 months of galactose feeding, retarded this cataract progression. Lenses of galactose-fed rats had increased Vit. E, lipid peroxide (LPO), galactitol, and water contents and decreased reduced glutathione (GSH) content at 6 months of feeding; increased LPO, galactitol, and water contents and decreased GSH content at 8 months. Sera of galactose-fed rats had increased Vit. E and cholesterol concentrations at 6 months of feeding. The liposome instillation increased lens Vit. E content with attenuation of the increased lens LPO content and the decreased lens GSH content but did not affect the changes in lens galactitol and water contents and serum Vit. E and cholesterol concentrations. These results indicate that instilled Vit. E-containing liposomes retard cataract progression in 12-month-old rats fed a 25% galactose diet, mainly by the antioxidative and membrane-stabilizing actions of Vit. E contained in the liposomes.
Ohta
Y
Y
Department of Chemistry, School of Medicine, Fujita Health University, Toyoake, Aichi, Japan. yohta@fujita-hu.ac.jp
Yamasaki
T
T
Niwa
T
T
Majima
Y
Y
D015060:1,2-Dipalmitoylphosphatidylcholine; D000818:Animals; D001834:Body Water / Q000378:metabolism; D002386:Cataract / Q000097:blood / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D002784:Cholesterol / Q000097:blood; D004032:Diet; D018450:Disease Progression; D004337:Drug Carriers; D004376:Galactitol / Q000378:metabolism; D005690:Galactose / Q000008:administration & dosage*; D005693:Galactosemias / Q000097:blood / Q000150:complications; D005978:Glutathione / Q000378:metabolism; D007908:Lens, Crystalline / Q000187:drug effects* / Q000473:pathology; D008054:Lipid Peroxides / Q000378:metabolism; D008081:Liposomes; D008297:Male; D010713:Phosphatidylcholines; D051381:Rats; D017208:Rats, Wistar; D014810:Vitamin E / Q000008:administration & dosage* / Q000097:blood
D004337:Drug Carriers; D008054:Lipid Peroxides; D008081:Liposomes; D010713:Phosphatidylcholines; D004376:Galactitol; D014810:Vitamin E; D015060:1,2-Dipalmitoylphosphatidylcholine; D002784:Cholesterol; C017251:1,2-oleoylphosphatidylcholine; D005978:Glutathione; D005690:Galactose