[The induction of the antitumor activity of T-lymphocytes by antigen-presenting cells obtained from the blast cells of patients with acute leukemias].
OBJECTIVE
To produce antigen-presenting cells (APC) from tumor cells of patients with acute leukemia and to induce autologous cytotoxic tumor-specific T-lymphocytes in vivo and in vitro.


METHODS
Culture examinations, flow cytofluorometry were made in 3 patients with acute myeloid leukemia (AML) and 1 patient with acute lymphoblastic leukemia (ALL).


RESULTS
Culturing of blast cells in the presence of growth factors produced APC in 4 patients with acute leukemia. The use of fibronectin layer with added collagen proved most effective for the above production. In culture, APC induced autologous tumor-specific T-lymphocytes which selectively lysed tumor cells. Pilot clinical trial was made of dendritic cells suspension in a patient with resistant AML.


CONCLUSIONS
A new perspective immunotherapy of acute leukemia is proposed.
Savchenko
V G
VG
Sadovnikova
E Iu
EIu
Parovichnikova
E N
EN
Mendeleeva
L P
LP
Demidova
I A
IA
Sokolov
A N
AN
Isaev
V G
VG
D000208:Acute Disease; D000938:Antigen-Presenting Cells / Q000276:immunology*; D002469:Cell Separation; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016219:Immunotherapy, Adoptive / Q000379:methods*; D007951:Leukemia, Myeloid / Q000276:immunology* / Q000628:therapy*; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology*; D008297:Male; D010641:Phenotype; D010865:Pilot Projects; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D013997:Time Factors; D014407:Tumor Cells, Cultured