Effects of mixed administration of glucose and fat emulsion on the liver of rats undergoing hepatopancreatectomy.
To prevent the development of liver failure after simultaneous massive resection of the liver and pancreas, we examined whether fat emulsion could be used as an energy source during postoperative transfusion. Using rats, the following four groups were prepared: the simple laparotomy group (S group), 68% hepatectomy group (H group), 90% pancreatectomy group (P group) and 68% hepatectomy + 90% pancreatectomy group (HP group). A constant total calorie level and transfusion dose were used. Three kinds of transfusions with different glucose-to-fat emulsion caloric ratios, that is: 75% fat emulsion (of the total calorie level) + 25% glucose (75L), 50% fat emulsion + 50% glucose (50L) and 25% fat emulsion + 75% glucose + 1 unit/5 g glucose of insulin (25L), were continuously administered for 48 hours, and the effects of these transfusions on the survival rate and liver were examined. After 75L administration, survival rates in the H group and HP group were significantly lower than those in the S group and P group. After administration of 50L, survival rates did not significantly differ among the four groups. After 25L administration, the survival rate in the HP group was significantly lower than that in other groups. In the HP group, the survival rate after 50L administration was the highest. However, necrosis was observed in a portion of the hepatic lobule, while the blood aspartate aminotransferase (AST) level was increased. After 25L administration, hepatocellular mitochondrial swelling and degeneration were noted in the HP group; furthermore, the blood total protein level was decreased. These results showed that the post-operative administration of fat emulsion at a percentage of 50% of the total calorie level caused partial hepatocellular necrosis in the HP group, but improved the survival rate.
Nakago
R
R
First Department of Surgery, Okayama University Medical School, Japan.
Tsuge
H
H
Harano
M
M
Kawata
N
N
Takakura
N
N
Tanaka
N
N
D000818:Animals; D005217:Fat Emulsions, Intravenous / Q000008:administration & dosage*; D005947:Glucose / Q000008:administration & dosage*; D006498:Hepatectomy / Q000379:methods*; D008099:Liver / Q000187:drug effects* / Q000601:surgery / Q000648:ultrastructure; D008111:Liver Function Tests; D008297:Male; D010180:Pancreatectomy / Q000379:methods*; D010289:Parenteral Nutrition, Total; D051381:Rats; D017207:Rats, Sprague-Dawley; D015996:Survival Rate
D005217:Fat Emulsions, Intravenous; D005947:Glucose