Comparison of global transcription responses allows identification of Vibrio cholerae genes differentially expressed following infection.
Comparison of global transcription profiles of Vibrio cholerae grown in vitro and in vivo revealed that 20% of the genome was repressed and about 5% was induced under in vivo conditions. Hybridization with the cloned genes revealed that the virulence genes ctx, toxR, toxT and tcpA were induced under in vivo conditions. Dissection of two in vivo induced cosmids identified another set of three genes homologous to che Y1 involved in motility and chemotaxis, pnuC encoding the major component of the nicotinamide mononucleotide transport system and icmF belonging to a cassette involved in multiplication inside host cells. These results demonstrate that the global transcription profile approach might be a powerful method for identification of differentially expressed transcripts under in vivo conditions.
Das
S
S
Biophysics Division, Indian Institute of Chemical Biology, Calcutta.
Chakrabortty
A
A
Banerjee
R
R
Roychoudhury
S
S
Chaudhuri
K
K
D000818:Animals; D002771:Cholera / Q000382:microbiology; D015169:Colony Count, Microbial; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D016680:Genome, Bacterial; D007082:Ileum / Q000382:microbiology*; D008969:Molecular Sequence Data; D011817:Rabbits; D014158:Transcription, Genetic; D014734:Vibrio cholerae / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics