title: The C. elegans gene lin-9,which acts in an Rb-related pathway, is required for gonadal sheath cell development and encodes a novel protein.
abstract: The Caenorhabditis elegans gene lin-9 functions in an Rb-related pathway that acts antagonistically to a receptor tyrosine kinase/Ras signal transduction pathway controlling vulval induction. We show that lin-9 is also required for the development of the sheath cells in the hermaphrodite gonad and for the development of the male spicule, rays and gonad. lin-9 is transcribed as two alternatively spliced 2.4kb messages, which use two distinct polyadenylation sites and are SL1 trans-spliced. The conceptual translation of lin-9 cDNA sequences predicts proteins of 642 and 644 amino acids with a significant similarity to predicted Drosophila and vertebrate proteins. We suggest that lin-9 is the founding member of a new protein family that functions in Rb-related pathways in many species.
authors.affiliation: Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: G J
authors.initials: GJ
authors.lastname: Beitel
authors.forename: E J
authors.initials: EJ
authors.lastname: Lambie
authors.forename: H R
authors.initials: HR
authors.lastname: Horvitz
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000166:cytology / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D012734:Disorders of Sex Development; D005260:Female; D017238:Genes, Helminth / Q000235:genetics; D005816:Genetic Complementation Test; D006066:Gonads / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D015801:Helminth Proteins / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D017354:Point Mutation; D018510:RNA, Helminth / Q000235:genetics; D016160:Retinoblastoma Protein / Q000235:genetics / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
chemical_list: D029742:Caenorhabditis elegans Proteins; D018076:DNA, Complementary; D015801:Helminth Proteins; C414732:LIN-9 protein, C elegans; D018510:RNA, Helminth; D016160:Retinoblastoma Protein; C121170:lin-36 protein, C elegans