[DNA ploidy and S phase in relation to metastases of pharyngolaryngeal epidermoid carcinoma].
Lymph node metastases in pharyngolaryngeal squamous-cell carcinoma are the clinical finding that most precisely condition prognosis. Sometimes the presence of subclinical metastases modifies the initial therapeutic strategy because they are not detected by physical exploration or imaging techniques. We tried to establish a relationship between the tumor's intrinsic aggressiveness and the development of metastases through tumoral DNA damage. Aneuploidy and the S phase were more frequent in tumors of the larynx, particularly supraglottic tumors, that developed lymph node metastases (72%) than in diploid tumors with a normal S phase (18%). In pharyngeal tumors, no differences could be established because most were aneuploid with a high S phase and metastases. Flow cytometry is highly sensitive, but not sufficiently specific for routine clinical use. However, it is a useful point of departure for our line of research, which will continue with a search for more specific genetic or molecular markers of metastases among the general DNA abnormalities.
Alvarez Marcos
C A
CA
Servicio de ORL, Hospital Valle del Nalón, Langreo.
Salas Bustamante
A
A
Sampedro Nuño
A
A
Suárez Nieto
C
C
Hevia Llama
R M
RM
Noval Menéndez
J
J
D000782:Aneuploidy; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000556:secondary; D004273:DNA, Neoplasm / Q000235:genetics*; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D010610:Pharyngeal Neoplasms / Q000235:genetics* / Q000556:secondary; D011003:Ploidies; D011446:Prospective Studies; D016196:S Phase / Q000502:physiology*; D012680:Sensitivity and Specificity
D004273:DNA, Neoplasm