title: Inactivation of 14-3-3sigma influences telomere behavior and ionizing radiation-induced chromosomal instability.
abstract: Telomeres are complexes of repetitive DNA sequences and proteins constituting the ends of linear eukaryotic chromosomes. While these structures are thought to be associated with the nuclear matrix, they appear to be released from this matrix at the time when the cells exit from G(2) and enter M phase. Checkpoints maintain the order and fidelity of the eukaryotic cell cycle, and defects in checkpoints contribute to genetic instability and cancer. The 14-3-3sigma gene has been reported to be a checkpoint control gene, since it promotes G(2) arrest following DNA damage. Here we demonstrate that inactivation of this gene influences genome integrity and cell survival. Analyses of chromosomes at metaphase showed frequent losses of telomeric repeat sequences, enhanced frequencies of chromosome end-to-end associations, and terminal nonreciprocal translocations in 14-3-3sigma(-/-) cells. These phenotypes correlated with a reduction in the amount of G-strand overhangs at the telomeres and an altered nuclear matrix association of telomeres in these cells. Since the p53-mediated G(1) checkpoint is operative in these cells, the chromosomal aberrations observed occurred preferentially in G(2) after irradiation with gamma rays, corroborating the role of the 14-3-3sigma protein in G(2)/M progression. The results also indicate that even in untreated cycling cells, occasional chromosomal breaks or telomere-telomere fusions trigger a G(2) checkpoint arrest followed by repair of these aberrant chromosome structures before entering M phase. Since 14-3-3sigma(-/-) cells are defective in maintaining G(2) arrest, they enter M phase without repair of the aberrant chromosome structures and undergo cell death during mitosis. Thus, our studies provide evidence for the correlation among a dysfunctional G(2)/M checkpoint control, genomic instability, and loss of telomeres in mammalian cells.
authors.affiliation: Center for Radiological Research, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA.
authors.forename: S
authors.initials: S
authors.lastname: Dhar
authors.forename: J A
authors.initials: JA
authors.lastname: Squire
authors.forename: M P
authors.initials: MP
authors.lastname: Hande
authors.forename: R J
authors.initials: RJ
authors.lastname: Wellinger
authors.forename: T K
authors.initials: TK
authors.lastname: Pandita
mesh_terms: D048948:14-3-3 Proteins; D014408:Biomarkers, Tumor; D002455:Cell Division / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D002843:Chromatin / Q000235:genetics / Q000528:radiation effects; D002871:Chromosome Banding; D019457:Chromosome Breakage / Q000235:genetics; D002873:Chromosome Fragility / Q000235:genetics*; D004307:Dose-Response Relationship, Radiation; D005092:Exonucleases; D005095:Exoribonucleases; D016193:G1 Phase; D016195:G2 Phase; D005720:Gamma Rays; D017353:Gene Deletion; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008940:Mitotic Index; D009363:Neoplasm Proteins; D015530:Nuclear Matrix / Q000378:metabolism; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics / Q000528:radiation effects; D012303:Ring Chromosomes; D016615:Telomere / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects*; D014178:Translocation, Genetic / Q000235:genetics / Q000528:radiation effects; D014407:Tumor Cells, Cultured
chemical_list: D048948:14-3-3 Proteins; D014408:Biomarkers, Tumor; D002843:Chromatin; D009363:Neoplasm Proteins; D011506:Proteins; D005092:Exonucleases; D005095:Exoribonucleases; C413442:SFN protein, human