title: Animal-cell damage in sparged bioreactors.
abstract: The gas sparging of culture broth causes damage to suspended animal cells. However, despite this, sparged bioreactors remain the preferred means of cell culture because sparging is a robust method of supplying oxygen, especially on a large scale. This article examines the underlying mechanisms involved in bubble-associated cell damage and the methods available for controlling such damage.
authors.affiliation: Department of Chemical Engineering, University of Almería, E-04071, Almería, Spain. ychisti@hotmail.com
authors.forename: Y
authors.initials: Y
authors.lastname: Chisti
mesh_terms: D000818:Animals; D019149:Bioreactors; D001769:Blood / Q000378:metabolism; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D003470:Culture Media; D007700:Kinetics; D008962:Models, Theoretical; D010100:Oxygen / Q000378:metabolism; D020442:Poloxamer / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000378:metabolism; D013501:Surface-Active Agents / Q000494:pharmacology
chemical_list: D003470:Culture Media; D013501:Surface-Active Agents; D020442:Poloxamer; D011092:Polyethylene Glycols; D010100:Oxygen