title: Ribonuclease T1 cleaves RNA after guanosines within single-stranded gaps of any length.
abstract: RNA-oligonucleotides with defined single-stranded stretches were designed to investigate the minimal requirements of a ribonuclease T1 substrate. It could be shown, that RNase T1 cleaves single-stranded RNA after a unique guanosine flanked by two double-stranded areas. However, the turnover of such a G-gap is significantly lower than that of a gap of two, three or four nucleotides.
authors.affiliation: Institut für Biochemie, Fakultät für Biowissenschaften, Pharmazie und Psychologie, Universität Leipzig, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Greiner-Stöffele
authors.forename: H H
authors.initials: HH
authors.lastname: Foerster
authors.forename: U
authors.initials: U
authors.lastname: Hahn
mesh_terms: D001483:Base Sequence; D006151:Guanosine / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009711:Nucleotides / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012313:RNA / Q000378:metabolism*; D006163:Ribonuclease T1 / Q000378:metabolism*
chemical_list: D009711:Nucleotides; D006151:Guanosine; D012313:RNA; D006163:Ribonuclease T1