Increased expression of H/T-cadherin in tumor-penetrating blood vessels.
Neovascularization is a prerequisite for tumor growth. Thus, selective destruction of the tumor vasculature should prevent tumor expansion. We have established a method to identify proteins that are specifically expressed on the surface of endothelial cells in tumors. CD31-positive endothelial cells were isolated from Lewis lung carcinoma lung metastases as well as from normal lung tissue. cDNAs derived from these cells were subjected to a subtractive hybridization procedure, and cDNAs overrepresented in tumor-derived endothelial cells were isolated; those encoding surface proteins were selected using a signal sequence trap assay. One isolated cDNA encoded H/T-cadherin. In this report, we show that mouse H/T-cadherin is overexpressed on endothelial cells of several tumors, whereas it is expressed only on a subset of endothelial cells in healthy organs. On the basis of the expression of H/T-cadherin in lung metastases of different tumors, we suggest that different tumors can have a differential influence on the expression of endothelial cell surface proteins.
Wyder
L
L
Department of Pathology, University Hospital, Zurich, Switzerland. l.wyder@icrf.icnet.uk
Vitaliti
A
A
Schneider
H
H
Hebbard
L W
LW
Moritz
D R
DR
Wittmer
M
M
Ajmo
M
M
Klemenz
R
R
D000595:Amino Acid Sequence; D000818:Animals; D015820:Cadherins / Q000096:biosynthesis* / Q000235:genetics; D018827:Carcinoma, Lewis Lung / Q000098:blood supply* / Q000235:genetics / Q000556:secondary; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D004273:DNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D004730:Endothelium, Vascular / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D008969:Molecular Sequence Data; D009368:Neoplasm Transplantation; D009389:Neovascularization, Pathologic / Q000378:metabolism*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
D015820:Cadherins; D018076:DNA, Complementary; D004273:DNA, Neoplasm; C099972:H-cadherin; D019408:Platelet Endothelial Cell Adhesion Molecule-1