title: Analysis of acyclovir by high performance capillary electrophoresis with on-column amperometric detection.
abstract: The separation of acyclovir (ACV) by high performance capillary electrophoresis (HPCE) with on-column amperometric detection using alpha-amino-5-mercapto-3,4-dithiazole (AMD) as internal standard is described. The calibration line was linear in the range of 0.5-20 mg/L of ACV. The detection limit was 0.15 mg/L of ACV. Its recovery ranged from 98 to 101% with relative standard deviations (RSDs) from 1.9 to 3.2% (n = 5). This method was successfully used for determining ACV in some pharmaceuticals and human urine. Comparable results with HPCE with ultraviolet (UV) detection and amperometric detection were obtained.
authors.affiliation: Center of Instrumental Analysis and Chemistry Department, Zhengzhou University, P.R. China. zhangshusheng@371.net
authors.forename: S
authors.initials: S
authors.lastname: Zhang
authors.forename: Z
authors.initials: Z
authors.lastname: Yuan
authors.forename: H
authors.initials: H
authors.lastname: Liu
authors.forename: H
authors.initials: H
authors.lastname: Zou
authors.forename: H
authors.initials: H
authors.lastname: Xiong
authors.forename: Y
authors.initials: Y
authors.lastname: Wu
mesh_terms: D000212:Acyclovir / Q000032:analysis* / Q000302:isolation & purification*; D000998:Antiviral Agents / Q000032:analysis* / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods; D006801:Humans
chemical_list: D000998:Antiviral Agents; D000212:Acyclovir