title: Bioavailability, biodistribution, and toxicity of BioZn-AAS(1): a new zinc source. comparative studies in rats.
abstract: Food fortification with a proper zinc compound is an economic and effective strategy to prevent zinc deficiency. BioZn-AAS, a zinc gluconate stabilized with glycine, was compared with zinc sulfate (reference standard), zinc hydroxide, and zinc gluconate, all of them labeled with (65)Zn. This preclinical study was performed on Sprague-Dawley rats of both sexes, and the administered dose was 85 microg/kg of zinc. Bioavailability studies showed that absorption of BioZn-AAS was not statistically different than absorption from other sources in female rats (25.65% +/- 2.20% for BioZn-AAS, 28.24% +/- 4. 60% for ZnSO(4), 24.91% +/- 4.02% for Zn[OH](2), and 25.51% +/- 2. 70% for Zn-gluconate). In the case of the male rats, absorption of BioZn-AAS (27.97% +/- 4.20%) was higher (P<0.05) than that from the other compounds (23.15% +/- 2.90% for ZnSO(4), 22.62% +/- 3.90% for Zn[OH](2), and 22.30% +/- 3.90% for Zn-gluconate). Biodistribution studies demonstrated that the zinc from BioZn-AAS followed the same metabolic pathway as zinc from the other sources. Toxicity studies were performed with 50 female and 50 male rats. The value of oral lethal dose 50 (LD(50)) was 2000 mg/kg for female rats and 1900 mg/kg for male rats. Therefore, we conclude that BioZn-AAS has adequate properties to be considered a proper zinc compound for food fortification or dietary supplementation.
authors.affiliation: Radioisotope Laboratory and the Physics Department, School of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina. jsalgueiro@huemul.ffyb.uba.ar
authors.forename: M J
authors.initials: MJ
authors.lastname: Salgueiro
authors.forename: M B
authors.initials: MB
authors.lastname: Zubillaga
authors.forename: A E
authors.initials: AE
authors.lastname: Lysionek
authors.forename: M I
authors.initials: MI
authors.lastname: Sarabia
authors.forename: R A
authors.initials: RA
authors.lastname: Caro
authors.forename: T
authors.initials: T
authors.lastname: De Paoli
authors.forename: A
authors.initials: A
authors.lastname: Hager
authors.forename: E
authors.initials: E
authors.lastname: Ettlin
authors.forename: R
authors.initials: R
authors.lastname: Weill
authors.forename: J R
authors.initials: JR
authors.lastname: Boccio
mesh_terms: D000042:Absorption; D000818:Animals; D001682:Biological Availability; D005260:Female; D005942:Gluconates / Q000493:pharmacokinetics; D006878:Hydroxides / Q000493:pharmacokinetics; D007928:Lethal Dose 50; D008297:Male; D009928:Organ Specificity; D051381:Rats; D017207:Rats, Sprague-Dawley; D017967:Zinc Compounds / Q000493:pharmacokinetics* / Q000633:toxicity*; D019287:Zinc Sulfate / Q000493:pharmacokinetics
chemical_list: D005942:Gluconates; D006878:Hydroxides; D017967:Zinc Compounds; D019287:Zinc Sulfate; C052745:zinc hydroxide