Comparison of abilities of Salmonella enterica serovar typhimurium aroA aroD and aroA htrA mutants to act as live vectors.
We compared the ability of Salmonella enterica serovar Typhimurium SL1344 aroA aroD (BRD509) and aroA htrA (BRD807) mutants to act as live vectors for delivery of fragment C of tetanus toxin (FrgC). FrgC was expressed in these strains from either pTETnir15 or pTEThtrA1. BRD509FrgC(+) strains elicited approximately 2-log-higher serum anti-FrgC antibody titers than BRD807FrgC(+) strains. All mice immunized with BRD807pTEThtrA1, BRD509pTEThtrA1, and BRD509pTETnir15 (but not BRD807pTETnir15) were protected against tetanus.
Roberts
M
M
Department of Veterinary Pathology, Glasgow University Veterinary School, Glasgow G61 1QH, United Kingdom. M.Roberts@vet.gla.ac.uk
Chatfield
S
S
Pickard
D
D
Li
J
J
Bacon
A
A
D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase; D019883:Alkyl and Aryl Transferases / Q000235:genetics*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001428:Bacterial Vaccines / Q000276:immunology; D005822:Genetic Vectors; D006360:Heat-Shock Proteins; D006836:Hydro-Lyases / Q000235:genetics*; D007114:Immunization; D051379:Mice; D009154:Mutation; D010446:Peptide Fragments / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D033903:Periplasmic Proteins; D012481:Salmonella Infections, Animal / Q000517:prevention & control; D012486:Salmonella typhimurium / Q000235:genetics* / Q000276:immunology; D012697:Serine Endopeptidases / Q000235:genetics*; D013742:Tetanus / Q000517:prevention & control; D013744:Tetanus Toxin / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D014613:Vaccines, Attenuated
D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D006360:Heat-Shock Proteins; D010446:Peptide Fragments; D033903:Periplasmic Proteins; D013744:Tetanus Toxin; D014613:Vaccines, Attenuated; C040635:tetanus toxin fragment C; D019883:Alkyl and Aryl Transferases; D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase; C061682:DegP protease; D012697:Serine Endopeptidases; D006836:Hydro-Lyases; C020000:3-dehydroquinate dehydratase