Orphanin FQ/nociceptin receptor binding studies.
A review of the binding studies performed on the receptor (ORL) for Orphanin FQ/Nociceptin is presented. Binding studies have been conducted using a variety of receptor sources: cell lines expressing the cloned receptor, cell lines endogenously expressing the receptor, and brain and other tissue from several different species. Binding studies of opioids, new ligands and antagonists at the ORL receptor are briefly discussed. Saturation, competition and binding kinetic experiments, and the effects of buffer composition are reviewed. There are numerous instances of conflicting data in published reports on OFQ; the basis for these disparities is as yet undetermined. This review endeavors to compile the results and conditions employed in binding studies as an aid to current and new researchers in this field. In an attempt to explain binding disparities, we have determined that Orphanin/Nociceptin binds to glass fiber filtermats in a "specific" manner; these new data are presented.
Dooley
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Torrey Pines Institute for Molecular Studies, 3550 General Atomics Court, 92121, San Diego, CA, USA. cdooley@tpims.org
Houghten
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D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism; D016466:CHO Cells; D002460:Cell Line; D003001:Cloning, Molecular; D006224:Cricetinae; D004399:Dynorphins / Q000737:chemistry / Q000378:metabolism; D006168:Guinea Pigs; D006801:Humans; D007700:Kinetics; D008024:Ligands; D051379:Mice; D008969:Molecular Sequence Data; D009292:Narcotic Antagonists; D010455:Peptides / Q000737:chemistry; D011485:Protein Binding; D051381:Rats; D011957:Receptors, Opioid / Q000737:chemistry* / Q000378:metabolism*; D013329:Structure-Activity Relationship; D000094942:Nociceptin Receptor
D008024:Ligands; D009292:Narcotic Antagonists; D010455:Peptides; D011957:Receptors, Opioid; D004399:Dynorphins; D000094942:Nociceptin Receptor