Comparison of sensitivity to ultraviolet B irradiation between human lymphocytes and hematopoietic stem cells.
To investigate the clinical applicability of prophylaxis of post-transplant graft-versus-host disease by UV-B irradiation of stem cell preparations, the UV-B sensitivities of human lymphocytes and primitive hematopoietic progenitors were compared. The mononuclear cell fractions (MNC) derived from human cord blood and granulocyte-colony-stimulating factor-mobilized peripheral blood were used. After UV-B irradiation, lymphocyte proliferation ability, hematopoietic colony-forming cells, and apoptotic cells were analyzed. At a dose of 33 J/m(2), significant differences were observed in the residual percentages of hematopoietic progenitors and lymphocyte functions [ANOVA, F (5,46) = 19.4; P <.0001], and the difference between CFU-C (85.2% + 24.0%; n = 8) and MLR (12.7% + 12. 6%; n = 10) was significant (P <.0001). There were no significant differences in the residual percentages of CFU-C, HPP-CFC, and LTC-IC. Percentages of annexin V-positive cells in the total MNC and the CD34(+) cell population in MNC after UV-B irradiation were 69.8% and 18.7%, respectively. In conclusion, there was a range of UV-B doses over which T lymphocytes were inactivated but hematopoietic progenitors, including HPP-CFC and LTC-IC, were preserved.
Azuma
H
H
Hokkaido Red Cross Blood Center, Sapporo; and the Nissho Corporation, Kusatsu, Japan. azuma@hokkaido.bc.jrc.or.jp
Ikebuchi
K
K
Yamaguchi
M
M
Murahashi
H
H
Mogi
Y
Y
Sato
N
N
Fujihara
M
M
Hirayama
F
F
Ikeda
H
H
D017304:Annexin A5 / Q000378:metabolism; D018952:Antigens, CD34 / Q000032:analysis; D017209:Apoptosis; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003208:Concanavalin A / Q000494:pharmacology; D005312:Fetal Blood / Q000166:cytology; D005434:Flow Cytometry; D006086:Graft vs Host Disease / Q000517:prevention & control; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000528:radiation effects*; D006801:Humans; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000528:radiation effects*; D014466:Ultraviolet Rays
D017304:Annexin A5; D018952:Antigens, CD34; D003208:Concanavalin A; D016179:Granulocyte Colony-Stimulating Factor