Participation of stress-inducible systems and enzymes involved in BER and NER in the protection of Escherichia coli against cumene hydroperoxide.
We studied the participation of the stress-inducible systems, as the OxyR, SoxRS and SOS regulons in the protection of Escherichia coli cells against lethal effects of cumene hydroperoxide (CHP). Moreover, we evaluated the participation of BER and NER in the repair of the DNA damage produced by CHP. Our results suggest that the hypersensitivity observed in the oxyR mutants to the lethal effect of CHP does not appear to be due to SOS inducing DNA lesions, but rather to cell membrane damage. On the other hand, DNA damage induced by CHP appears to be repaired by enzymes involved in BER and NER pathways. In this case, Fpg protein and UvrABC complex could be involved cooperatively in the elimination of a specific DNA lesion. Finally, we have detected the requirement for the uvrA gene function in SOS induction by CHP treatment.
Asad
L M
LM
Departamento de Biofísica e Biometria, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, 20551-030 Rio de Janeiro, RJ, Brazil.
Medeiros
D C
DC
Felzenszwalb
I
I
Leitão
A C
AC
Asad
N R
NR
D016587:Antimutagenic Agents; D001426:Bacterial Proteins; D001555:Benzene Derivatives / Q000009:adverse effects*; D004249:DNA Damage; D004260:DNA Repair; D004268:DNA-Binding Proteins; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D029968:Escherichia coli Proteins; D017353:Gene Deletion; D009153:Mutagens / Q000494:pharmacology; D009154:Mutation; D016877:Oxidants / Q000009:adverse effects*; D012097:Repressor Proteins / Q000235:genetics; D013014:SOS Response, Genetics; D013481:Superoxides / Q000494:pharmacology; D015534:Trans-Activators; D014157:Transcription Factors / Q000235:genetics
D016587:Antimutagenic Agents; D001426:Bacterial Proteins; D001555:Benzene Derivatives; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D009153:Mutagens; D016877:Oxidants; D012097:Repressor Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C499299:oxyR protein, E coli; D013481:Superoxides; C070724:SoxR protein, Bacteria; C070725:SoxS protein, E coli; C007164:cumene hydroperoxide