title: Analysis of EEG and CSF 14-3-3 proteins as aids to the diagnosis of Creutzfeldt-Jakob disease.
abstract: OBJECTIVE
  To improve diagnostic criteria for sporadic Creutzfeldt-Jakob disease (CJD).
  
  
  METHODS
  Pooled data on initial and final diagnostic classification of suspected CJD patients were accumulated, including results of investigations derived from a coordinated multinational study of CJD. Prospective analysis for a comparison of clinical and neuropathologic diagnoses and evaluation of the sensitivity and specificity of EEG and 14-3-3 CSF immunoassay were conducted.
  
  
  RESULTS
  Data on 1,003 patients with suspected CJD were collected using a standard questionnaire. After follow-up was carried out, complete clinical data and neuropathologic diagnoses were available in 805 cases. In these patients, the sensitivity of the detection of periodic sharp wave complexes in the EEG was 66%, with a specificity of 74%. The detection of 14-3-3 proteins in the CSF correlated with the clinical diagnosis in 94% (sensitivity). The specificity (84%) was higher than that of EEG. A combination of both investigations further increased the sensitivity but decreased the specificity.
  
  
  CONCLUSIONS
  Incorporation of CSF 14-3-3 analysis in the diagnostic criteria for CJD significantly increases the sensitivity of case definition. Amended diagnostic criteria for CJD are proposed.
authors.affiliation: Klinik und Poliklinik für Neurologie, Georg-August-Universität, Göttingen, Germany.
authors.forename: I
authors.initials: I
authors.lastname: Zerr
authors.forename: M
authors.initials: M
authors.lastname: Pocchiari
authors.forename: S
authors.initials: S
authors.lastname: Collins
authors.forename: J P
authors.initials: JP
authors.lastname: Brandel
authors.forename: J
authors.initials: J
authors.lastname: de Pedro Cuesta
authors.forename: R S
authors.initials: RS
authors.lastname: Knight
authors.forename: H
authors.initials: H
authors.lastname: Bernheimer
authors.forename: F
authors.initials: F
authors.lastname: Cardone
authors.forename: N
authors.initials: N
authors.lastname: Delasnerie-Lauprêtre
authors.forename: N
authors.initials: N
authors.lastname: Cuadrado Corrales
authors.forename: A
authors.initials: A
authors.lastname: Ladogana
authors.forename: M
authors.initials: M
authors.lastname: Bodemer
authors.forename: A
authors.initials: A
authors.lastname: Fletcher
authors.forename: T
authors.initials: T
authors.lastname: Awan
authors.forename: A
authors.initials: A
authors.lastname: Ruiz Bremón
authors.forename: H
authors.initials: H
authors.lastname: Budka
authors.forename: J L
authors.initials: JL
authors.lastname: Laplanche
authors.forename: R G
authors.initials: RG
authors.lastname: Will
authors.forename: S
authors.initials: S
authors.lastname: Poser
mesh_terms: D048948:14-3-3 Proteins; D001921:Brain / Q000503:physiopathology*; D007562:Creutzfeldt-Jakob Syndrome / Q000134:cerebrospinal fluid* / Q000503:physiopathology*; D004569:Electroencephalography; D006801:Humans; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis*
chemical_list: D048948:14-3-3 Proteins; D014446:Tyrosine 3-Monooxygenase