Optimisation of procedures for the extraction of structural analogues of propranolol with molecular imprinted polymers for sample preparation.
A propranolol-derived molecular imprinted polymer (MIP) was prepared using methacrylic acid as monomer and ethylene glycol dimethacrylate as cross-linker. The extraction properties of five compounds structurally related to propranolol were assessed on the MIP and on a blank polymer made under the same conditions but in the absence of an imprint molecule. Using application from aqueous solution with methanol-water-triethylamine (TEA)-based solvents for elution (i.e. reversed-phase conditions) the MIP showed only marginal selectivity for the compounds on the MIP compared to the blank. Despite the limited selectivity there did appear to be a relationship between structure of the compound (relative to propranolol) and the extent of selective retention. Application of the compounds in toluene with elution using toluene-TEA or toluene-trifluoroacetic acid resulted in the MIP showing dramatically enhanced retention and selectivity of the compounds on the MIP compared to the blank. The enhanced selectivity for extraction on to the MIP relative to the blank, for all compounds using normal-phase solvents seem to be a class effect as there was no apparent relationship between compound structure and retention.
Martin
P
P
Department of Safety of Medicines, AstraZeneca UK Ltd., Macclesfield, Cheshire.
Wilson
I D
ID
Jones
G R
GR
D000319:Adrenergic beta-Antagonists / Q000737:chemistry / Q000302:isolation & purification; D019411:Clinical Laboratory Techniques; D011108:Polymers / Q000737:chemistry*; D011433:Propranolol / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification*; D012997:Solvents / Q000737:chemistry
D000319:Adrenergic beta-Antagonists; D011108:Polymers; D012997:Solvents; D011433:Propranolol