title: Sulfated fibroin, a novel sulfated peptide derived from silk, inhibits human immunodeficiency virus replication in vitro.
abstract: We prepared two kinds of sulfated silk fibroins, SclFib30 and SclFib31, which contain different amounts of sulfate. These sulfated silk fibroins have anti-HIV-1 activity in vitro, apparently due to interference with the adsorption of virus particles to CD4+ cells, and completely blocked virus binding to the cells at a concentration of 100 microg/ml. Sulfated fibroins also abolished cell-to-cell infection-induced syncytium formation upon cocultivation of MOLT-4 and MOLT-4/HIV-IIIB cells, suggesting that they would interfere with gp120 and prevent the formation of gp120/CD4 complex. Silk is used in biomaterials such as surgical sutures and is believed to be a safe material for humans. In accordance with low anticoagulant activity and high anti-HIV-1 activity against both X4 HIV-1 and R5 HIV-1 strains, sulfated silk fibroins have potential as antiviral material such for a vaginal anti-HIV formulation.
authors.affiliation: Department of Microbiology and Immunology, Kagoshima University Dental School, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Gotoh
authors.forename: H
authors.initials: H
authors.lastname: Izumi
authors.forename: T
authors.initials: T
authors.lastname: Kanamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Tamada
authors.forename: H
authors.initials: H
authors.lastname: Nakashima
mesh_terms: D000818:Animals; D019380:Anti-HIV Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000925:Anticoagulants / Q000378:metabolism; D012831:Bombyx; D002450:Cell Communication / Q000187:drug effects; D002460:Cell Line; D005260:Female; D005349:Fibroins / Q000302:isolation & purification / Q000494:pharmacology*; D015497:HIV-1 / Q000187:drug effects* / Q000472:pathogenicity / Q000502:physiology; D006801:Humans; D019476:Insect Proteins / Q000737:chemistry*; D047011:Silk; D013431:Sulfates / Q000378:metabolism; D013537:Sutures; D014621:Vagina / Q000821:virology; D014771:Virion; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D019380:Anti-HIV Agents; D000925:Anticoagulants; D019476:Insect Proteins; D047011:Silk; D013431:Sulfates; D005349:Fibroins