Molecular cloning and expression of a phospholipid hydroperoxide glutathione peroxidase homolog in Oryza sativa.
A cDNA encoding putative phospholipid hydroperoxide glutathione peroxidase (PHGPX) was isolated from rice using rapid amplification of cDNA ends. This cDNA, designated ricPHGPX, includes an open reading frame encoding a protein of 169 amino acids which shares about 60% and 50% amino acid sequence identity with plant and mammalian PHGPXs, respectively. The gene is expressed at a relative high level in flag leaves and the expression can be markedly induced by oxidative stress, suggesting that the product of the gene plays a key role in defense against oxidative damage in rice.
Li
W J
WJ
Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, PR China. liujy@mail.tsinghua.edu.cn
Feng
H
H
Fan
J H
JH
Zhang
R Q
RQ
Zhao
N M
NM
Liu
J Y
JY
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D005786:Gene Expression Regulation; D017343:Genes, Plant; D005979:Glutathione Peroxidase / Q000235:genetics*; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology / Q000235:genetics*; D018384:Oxidative Stress; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D016415:Sequence Alignment; D017385:Sequence Homology
D018076:DNA, Complementary; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D005979:Glutathione Peroxidase