DNA encoding the attachment (G) or fusion (F) protein of respiratory syncytial virus induces protection in the absence of pulmonary inflammation.
Significant protection against respiratory syncytial virus (RSV) infection was induced in mice vaccinated intramuscularly (i.m.) with DNA encoding the F or G protein of RSV. The amounts of IgG1 of IgG2a antibodies in mice immunized with DNA-G alone were similar. However, the antibody response in mice co-immunized with DNA-G and DNA encoding IL-4 (DNA-IL-4) was strongly biased towards IgG1. In contrast, the antibody response in mice co-immunized with DNA-G and DNA-IL-2, -IL-12 or-IFN-gamma was biased towards IgG2a. Mice vaccinated with DNA-F either alone or in combination with DNA encoding cytokines developed a predominant RSV-specific IgG2a response, which was most pronounced in mice co-immunized with DNA-F and DNA-IL-12 or -IFN-gamma. Vaccinated mice developed only a slightly enhanced pulmonary inflammatory response following RSV challenge. More significantly, and in contrast to mice scarified with recombinant vaccinia virus expressing the G protein, mice vaccinated i.m. with DNA-G did not develop pulmonary eosinophilia, even when the immune response was biased towards a Th2 response by co-administration of DNA-IL-4.
Bembridge
Gary P
GP
Institute for Animal Health, Compton, Newbury, Berkshire RG20 7NN , UK1.
Rodriguez
Nuria
N
Centro Nacional de Biologia Fundamental, Instituto de Salud Carlos III, Majadahonda, 28220 Madrid, Spain2.
Garcia-Beato
Regina
R
Centro Nacional de Biologia Fundamental, Instituto de Salud Carlos III, Majadahonda, 28220 Madrid, Spain2.
Nicolson
Carolyn
C
NIBSC, Blanche Lane, South Mimms, Potters Bar, Hertfordshire EN6 3QG, UK3.
Melero
Jose A
JA
Centro Nacional de Biologia Fundamental, Instituto de Salud Carlos III, Majadahonda, 28220 Madrid, Spain2.
Taylor
Geraldine
G
Institute for Animal Health, Compton, Newbury, Berkshire RG20 7NN , UK1.
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000956:Antigens, Viral / Q000235:genetics*; D004195:Disease Models, Animal; D007371:Interferon-gamma / Q000494:pharmacology; D018664:Interleukin-12 / Q000494:pharmacology; D051379:Mice; D011024:Pneumonia, Viral / Q000517:prevention & control* / Q000821:virology*; D011657:Pulmonary Eosinophilia / Q000517:prevention & control / Q000821:virology; D018357:Respiratory Syncytial Virus Infections / Q000517:prevention & control*; D012136:Respiratory Syncytial Viruses / Q000235:genetics*; D018418:Th2 Cells / Q000276:immunology / Q000821:virology; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins / Q000235:genetics*; D014760:Viral Fusion Proteins / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; D014764:Viral Proteins; D018664:Interleukin-12; D007371:Interferon-gamma