title: Interleukin-4 (IL-4) and IL-13 signaling pathways do not regulate Borrelia burgdorferi-induced arthritis in mice: IgG1 is not required for host control of tissue spirochetes.
abstract: Previous studies have suggested that interleukin-4 (IL-4) has a protective effect in host defense to Borrelia burgdorferi infection, both in limiting the severity of arthritis and in controlling spirochete numbers in tissues, and a mapping study revealed suggestive linkage to a cluster of genes on mouse chromosome 11, including the genes for IL-4 and IL-13. In contrast, other studies have questioned the importance of IL-4. In this study the involvement of IL-4 in murine Lyme disease was examined in C57BL/6J and BALB/cJ mice with targeted disruptions in the IL-4 gene, the IL-4Ralpha chain gene, or both. A spectrum of arthritis severity was seen in BALB/cJ mice, and ablation of IL-4, IL-4Ralpha, or both had no effect on the overall severity of arthritis as determined by joint swelling and histopathology. Wild-type C57BL/6J mice exhibited mild to moderate arthritis, and ablation of IL-4 again had no effect on arthritis severity. IL-4- and IL-4Ralpha-deficient mice produced extremely low levels of immunoglobulin G1 (IgG1) and showed increased production of IgG2b. This shift in immunoglobulin isotype had no effect on the host's ability to control spirochete growth in either strain of mouse, as determined by PCR detection of B. burgdorferi DNA from heart and ankle tissues. In summary, the IL-4-IL-4Ralpha pathway, including IL-13 signaling, neither limits arthritis severity nor is required for control of spirochete growth during B. burgdorferi infection of mice. Furthermore, the IgG1 isotype is not required to control B. burgdorferi cell numbers in tissues. These findings suggest the host defense against B. burgdorferi infection is not dependent on the Th1-Th2 paradigm of T-cell responses.
authors.affiliation: Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah 84132, USA.
authors.forename: M R
authors.initials: MR
authors.lastname: Potter
authors.forename: N
authors.initials: N
authors.lastname: Noben-Trauth
authors.forename: J H
authors.initials: JH
authors.lastname: Weis
authors.forename: C
authors.initials: C
authors.lastname: Teuscher
authors.forename: J J
authors.initials: JJ
authors.lastname: Weis
mesh_terms: D000818:Animals; D001170:Arthritis, Infectious / Q000276:immunology* / Q000382:microbiology; D015748:Borrelia burgdorferi Group / Q000276:immunology*; D005260:Female; D017353:Gene Deletion; D006321:Heart / Q000382:microbiology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology*; D018793:Interleukin-13 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D015847:Interleukin-4 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D007596:Joints / Q000382:microbiology; D008193:Lyme Disease / Q000276:immunology* / Q000382:microbiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D015398:Signal Transduction
chemical_list: D007074:Immunoglobulin G; D018793:Interleukin-13; D015847:Interleukin-4