Methionine interference in rapamycin production involves repression of demethylrapamycin methyltransferase and S-adenosylmethionine synthetase.
In a chemically defined medium, L-methionine decreased production of rapamycin and increased that of demethylrapamycin. Growth with L-methionine yielded cells with a lower ability to convert demethylrapamycin to rapamycin and decreased the level of S-adenosylmethionine synthetase and S-adenosylmethionine. Thus, methionine represses at least one methyltransferase of rapamycin biosynthesis and S-adenosylmethionine synthetase.
Kim
W S
WS
Fermentation Microbiology Laboratory, Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Wang
Y
Y
Fang
A
A
Demain
A L
AL
D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D008274:Magnesium / Q000378:metabolism; D008715:Methionine / Q000494:pharmacology*; D008716:Methionine Adenosyltransferase / Q000037:antagonists & inhibitors*; D008780:Methyltransferases / Q000037:antagonists & inhibitors*; D020123:Sirolimus / Q000378:metabolism*; D013302:Streptomyces / Q000201:enzymology / Q000378:metabolism
D008715:Methionine; D008780:Methyltransferases; C415968:demethylrapamycin methyltransferase; D008716:Methionine Adenosyltransferase; D008274:Magnesium; D020123:Sirolimus