title: Detection of the high-level aminoglycoside resistance gene aph(2")-Ib in Enterococcus faecium.
abstract: A new high-level gentamicin resistance gene, designated aph(2")-Ib, was cloned from Enterococcus faecium SF11770. The deduced amino acid sequence of the 897-bp open reading frame of aph(2")-Ib shares homology with the aminoglycoside-modifying enzymes AAC(6')-APH(2"), APH(2")-Ic, and APH(2")-Id. The observed phosphotransferase activity is designated APH(2")-Ib.
authors.affiliation: Research and Medical Service, John D. Dingell VA Medical Center, and Wayne State University School of Medicine, Detroit, Michigan 48201, USA.
authors.forename: S J
authors.initials: SJ
authors.lastname: Kao
authors.forename: I
authors.initials: I
authors.lastname: You
authors.forename: D B
authors.initials: DB
authors.lastname: Clewell
authors.forename: S M
authors.initials: SM
authors.lastname: Donabedian
authors.forename: M J
authors.initials: MJ
authors.lastname: Zervos
authors.forename: J
authors.initials: J
authors.lastname: Petrin
authors.forename: K J
authors.initials: KJ
authors.lastname: Shaw
authors.forename: J W
authors.initials: JW
authors.lastname: Chow
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial; D016984:Enterococcus faecium / Q000187:drug effects* / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D005839:Gentamicins / Q000494:pharmacology; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000096:biosynthesis* / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D005839:Gentamicins; D017853:Phosphotransferases (Alcohol Group Acceptor); C415966:aph(2')-Ib protein, Enterococcus faecium