title: Hyperthermia increases accumulation of technetium-99m-labeled liposomes in feline sarcomas.
abstract: The effect of hyperthermia on the accumulation of technetium-99m-labeled liposomes was studied in feline sarcomas. Each cat received two separate injections of liposomes. The first was used to quantify the amount of technetium-99m-labeled liposomes within the tumor under normothermic conditions. The second injection was made at the beginning of a 60-min hyperthermia procedure. Planar scintigraphy was used to measure the activity of technetium-99m-labeled liposomes within the tumor at predetermined times up to 18 h after injection. Regions of interest were drawn for the tumor, lungs, liver, kidney, and aorta. Counts in the regions of interest were decay corrected. Counts/pixel in the tumor under normothermic and hyperthermic conditions were normalized to aorta counts/pixel. A total of 16 cats were eligible for the study. In two of the 16 cats, incomplete count data precluded analysis. In the remaining 14 cats, hyperthermia resulted in a significant increase in liposome accumulation in the tumor (P = 0.001). Tumor volume ranged from 1.2 to 236.2 cm3, and thermal dose ranged from 2.0 to 243.3 CEM43CT90 (equivalent time that the 10th percentile temperature was equal to 43 degrees C). There was not a relationship between either tumor volume or hyperthermia dose on the magnitude of increased liposome accumulation, suggesting that this method has application across a range of tumor volumes and degrees of heatibility.
authors.affiliation: North Carolina State University, Raleigh 27606, USA.
authors.forename: M L
authors.initials: ML
authors.lastname: Matteucci
authors.forename: G
authors.initials: G
authors.lastname: Anyarambhatla
authors.forename: G
authors.initials: G
authors.lastname: Rosner
authors.forename: C
authors.initials: C
authors.lastname: Azuma
authors.forename: P E
authors.initials: PE
authors.lastname: Fisher
authors.forename: M W
authors.initials: MW
authors.lastname: Dewhirst
authors.forename: D
authors.initials: D
authors.lastname: Needham
authors.forename: D E
authors.initials: DE
authors.lastname: Thrall
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000000981:diagnostic imaging / Q000378:metabolism*; D002415:Cats; D005354:Fibrosarcoma / Q000000981:diagnostic imaging / Q000378:metabolism / Q000662:veterinary*; D006979:Hyperthermia, Induced; D007553:Isotope Labeling; D008081:Liposomes / Q000737:chemistry / Q000493:pharmacokinetics*; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics*; D012983:Soft Tissue Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism / Q000662:veterinary*; D013667:Technetium / Q000493:pharmacokinetics*; D019690:Technetium Tc 99m Exametazime / Q000737:chemistry; D014018:Tissue Distribution
chemical_list: D008081:Liposomes; D019275:Radiopharmaceuticals; D019690:Technetium Tc 99m Exametazime; D013667:Technetium