Variations in the major envelope glycoprotein GP5 of Czech strains of porcine reproductive and respiratory syndrome virus.
The major envelope glycoprotein genes (ORF5) of seven Czech isolates of porcine reproductive and respiratory syndrome virus (PRRSV) were amplified and their nucleotide sequences were determined. ORF5 displayed nucleotide and amino acid identities of 87.5-100% and 87. 6-100%, respectively, among the isolates. In a phylogenetic tree, all European isolates were grouped in a genotype distinct from that of reference American strains (VR-2332, IAF-Klop). Among the European isolates, two different clades were identified. Two Czech isolates (V-501 and V-503) and Italian strain PRRSV 2156 fell into one clade. The remaining European strains comprised the second clade. Surprisingly, two separately clustered strains (V-501 and V-516) were isolated from the same herd. Additionally, the possible effect of in vitro cultivation on the nucleotide sequence was analysed. Nine point mutations in the ORF5 region resulted from 152 in vitro passages of the V-502 isolate in MARC-145 cells.
Indik
Stanislav
S
Veterinary Research Institute, Hudcova 70, Brno 621 32, Czech Republic1.
Valíček
Lubomír
L
Veterinary Research Institute, Hudcova 70, Brno 621 32, Czech Republic1.
Klein
Dieter
D
University of Veterinary Sciences, Veterinärplatz 1, A-1210 Vienna, Austria2.
Klánová
Jana
J
Faculty of Science, Masaryk University, Kotlárská 2, Brno 611 37, Czech Republic3.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018153:Czech Republic; D004279:DNA, Viral / Q000737:chemistry; D008562:Membrane Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D017354:Point Mutation; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics*; D016415:Sequence Alignment; D013552:Swine; D014759:Viral Envelope Proteins / Q000235:genetics*
D004279:DNA, Viral; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins; C114789:glycoprotein 5, PRRSV