title: Co-expression of human chaperone Hsp70 and Hsdj or Hsp40 co-factor increases solubility of overexpressed target proteins in insect cells.
abstract: The insect-baculovirus expression system has proved particularly useful for producing recombinant proteins that are biologically active. Overexpression of foreign proteins using the recombinant baculovirus system is often accompanied by aggregation of the overexpressed protein, which is thought to be due to a limitation of the translated protein folding in the infected cells. Co-infection of a recombinant baculovirus capable of expressing the human chaperone Hsp70 slightly increased the solubility of the overexpressed Epstein-Barr virus replication protein, BZLF1. Co-expression of Hsp70 and its co-factor, Hsdj or Hsp40, was here found to improve the solubility of the target protein several fold. Thus, a baculovirus expression system producing these molecular chaperones may find application for improved production of target foreign gene products in insect cells.
authors.affiliation: Division of Virology, Aichi Cancer Center Research Institute, 1-1 Kanokoden, Chikusa-ku, Nagoya 464-8681, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Yokoyama
authors.forename: M
authors.initials: M
authors.lastname: Hirata
authors.forename: K
authors.initials: K
authors.lastname: Ohtsuka
authors.forename: Y
authors.initials: Y
authors.lastname: Nishiyama
authors.forename: K
authors.initials: K
authors.lastname: Fujii
authors.forename: M
authors.initials: M
authors.lastname: Fujita
authors.forename: K
authors.initials: K
authors.lastname: Kuzushima
authors.forename: T
authors.initials: T
authors.lastname: Kiyono
authors.forename: T
authors.initials: T
authors.lastname: Tsurumi
mesh_terms: D000818:Animals; D016367:Baculoviridae; D002352:Carrier Proteins / Q000096:biosynthesis*; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005821:Genetic Techniques; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis*; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D004854:Herpesvirus 4, Human; D006801:Humans; D019476:Insect Proteins / Q000096:biosynthesis* / Q000737:chemistry; D012995:Solubility; D018411:Spodoptera; D015534:Trans-Activators / Q000096:biosynthesis / Q000737:chemistry; D014764:Viral Proteins / Q000096:biosynthesis / Q000737:chemistry
chemical_list: C063676:BZLF1 protein, Herpesvirus 4, Human; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; C107481:DNAJA1 protein, human; C494647:DNAJB1 protein, human; D050956:HSP40 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D019476:Insect Proteins; D015534:Trans-Activators; D014764:Viral Proteins