title: Interaction of flavodoxin with cobalamin-dependent methionine synthase.
abstract: Cobalamin-dependent methionine synthase catalyzes the transfer of a methyl group from methyltetrahydrofolate to homocysteine, forming tetrahydrofolate and methionine. The Escherichia coli enzyme, like its mammalian homologue, is occasionally inactivated by oxidation of the cofactor to cob(II)alamin. To return to the catalytic cycle, the cob(II)alamin forms of both the bacterial and mammalian enzymes must be reductively remethylated. Reduced flavodoxin donates an electron for this reaction in E. coli, and S-adenosylmethionine serves as the methyl donor. In humans, the electron is thought to be provided by methionine synthase reductase, a protein containing a domain with a significant degree of homology to flavodoxin. Because of this homology, studies of the interactions between E. coli flavodoxin and methionine synthase provide a model for the mammalian system. To characterize the binding interface between E. coli flavodoxin and methionine synthase, we have employed site-directed mutagenesis and chemical cross-linking using carbodiimide and N-hydroxysuccinimide. Glutamate 61 of flavodoxin is identified as a cross-linked residue, and lysine 959 of the C-terminal activation domain of methionine synthase is assigned as its partner. The mutation of lysine 959 to threonine results in a diminished level of cross-linking, but has only a small effect on the affinity of methionine synthase for flavodoxin. Identification of these cross-linked residues provides evidence in support of a docking model that will be useful in predicting the effects of mutations observed in mammalian homologues of E. coli flavodoxin and methionine synthase.
authors.affiliation: Biophysics Research Division and Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109-1055, USA.
authors.forename: D A
authors.initials: DA
authors.lastname: Hall
authors.forename: T C
authors.initials: TC
authors.lastname: Jordan-Starck
authors.forename: R O
authors.initials: RO
authors.lastname: Loo
authors.forename: M L
authors.initials: ML
authors.lastname: Ludwig
authors.forename: R G
authors.initials: RG
authors.lastname: Matthews
mesh_terms: D013766:5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D001665:Binding Sites / Q000235:genetics; D003432:Cross-Linking Reagents / Q000378:metabolism; D004789:Enzyme Activation / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000378:metabolism; D005418:Flavodoxin / Q000737:chemistry / Q000378:metabolism*; D008239:Lysine / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016297:Mutagenesis, Site-Directed; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013388:Succinimides / Q000378:metabolism; D014805:Vitamin B 12 / Q000737:chemistry / Q000378:metabolism
chemical_list: D003432:Cross-Linking Reagents; D005418:Flavodoxin; D046911:Macromolecular Substances; D010446:Peptide Fragments; D013388:Succinimides; D013766:5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase; D008239:Lysine; C001426:N-hydroxysuccinimide; D014805:Vitamin B 12; D005022:Ethyldimethylaminopropyl Carbodiimide