title: Early alteration of nucleocytoplasmic traffic induced by some RNA viruses.
abstract: A HeLa cell line expressing the green fluorescent protein fused to the SV40 T-antigen nuclear localization signal (EGFP-NLS) was established. Fluorescence in these cells was confined to the nuclei. After poliovirus infection, cytoplasmic fluorescence in a proportion of cells could be detected by 1 h postinfection (p.i.) and in virtually all of the fluorescent cells by 2 h p.i. The relocation could be prevented by cycloheximide but not by inhibition of poliovirus replication by guanidine. HCl. Nuclear exit of a protein composed of three copies of GFP fused to the NLS also occurred upon poliovirus infection. A similar redistribution of EGFP-NLS took place upon infection with coxsakievirus B3 and, to a lesser extent, with vesicular stomatitis virus. The EGFP-NLS efflux was not due to the loss of NLS. Thus, some positive-strand and negative-strand RNA viruses trigger a rapid nonspecific relocation of nuclear proteins.
authors.affiliation: M. P. Chumakov Institute of Poliomyelitis and Viral Encephalitides, Russian Academy of Medical Sciences, Moscow Region, Russia.
authors.forename: G A
authors.initials: GA
authors.lastname: Belov
authors.forename: A G
authors.initials: AG
authors.lastname: Evstafieva
authors.forename: Y P
authors.initials: YP
authors.lastname: Rubtsov
authors.forename: O V
authors.initials: OV
authors.lastname: Mikitas
authors.forename: A B
authors.initials: AB
authors.lastname: Vartapetian
authors.forename: V I
authors.initials: VI
authors.lastname: Agol
mesh_terms: D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D001692:Biological Transport; D015153:Blotting, Western; D002467:Cell Nucleus / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D029822:Enterovirus B, Human / Q000235:genetics / Q000378:metabolism*; D049452:Green Fluorescent Proteins; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics; D008856:Microscopy, Fluorescence; D019913:Nuclear Localization Signals / Q000235:genetics / Q000378:metabolism*; D017955:Poliovirus / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics / Q000378:metabolism*
chemical_list: D000952:Antigens, Polyomavirus Transforming; D008164:Luminescent Proteins; D019913:Nuclear Localization Signals; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins