Progesterone and follicular changes in postpartum noncyclic dairy cows after treatment with progesterone and estradiol or with progesterone, GnRH, PGF2alpha, and estradiol.
A previous study showed that noncyclic dairy cows treated with 10 microg of GnRH and a progesterone-releasing CIDR insert on Day 0, 25 mg of PGF2alpha and CIDR removal on Day 7, followed by 1 mg estradiol benzoate on Day 9 for those cows that still had not shown estrus (CGPE program) had higher conception rate (47% vs. 29%) than cows treated only with CIDR and estradiol benzoate as above (CE program). This study was to investigate the mechanisms by which the CGPE program improved conception rate compared with the CE program. Sixteen noncyclic Holstein-Friesian cows were randomly assigned to 2 groups balanced for the size and growth pattern of the dominant follicles, which were determined by ultrasonography over a 3-d period. One group received the above CGPE treatment, and the other group received the CE treatment. Follicular and luteal development were monitored by daily ultrasonography. Blood samples were collected daily from Day -2 to Day 11, and thereafter milk samples were collected thrice weekly for a further 24 d. Blood and milk samples were analyzed for progesterone. The GnRH treatment induced ovulation in 7 of 8 cows, resulting in elevated (P<0.05) progesterone concentrations between Days 4 and 7 for cows in the CGPE group. All induced CL underwent luteolysis by 24 h after PGF2alpha treatment. Within 5 d of CIDR removal, 7 of 8 cows in both the CE and CGPE groups ovulated. The interval from emergence of the ovulatory follicle to ovulation was similar (P=0.32) but less (P<0.05) variable for the CGPE group (9.0+/-0.3 d) compared with the CE group (10.3+/-1.2 d). Progesterone concentration in milk samples was similar between the two groups up to 10 d after ovulation. In summary, the GnRH treatment induced ovulation or turnover of dominant follicles, induced a synchronized initiation of a new follicular wave, and increased the progesterone concentration from 4 d after treatment. These could be the reasons for the increased conception rate of cows treated with the CGPE program.
Xu
Z Z
ZZ
Livestock Improvement Corporation, Hamilton, New Zealand. zxu@lic.co.nz
Verkerk
G A
GA
Mee
J F
JF
Morgan
S R
SR
Clark
Burke
C R
CR
Burton
L J
LJ
D000818:Animals; D002417:Cattle / Q000502:physiology*; D015237:Dinoprost / Q000008:administration & dosage / Q000502:physiology; D004958:Estradiol / Q000008:administration & dosage / Q000097:blood / Q000502:physiology*; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000008:administration & dosage / Q000502:physiology; D008297:Male; D008892:Milk / Q000737:chemistry; D006080:Ovarian Follicle / Q000000981:diagnostic imaging / Q000187:drug effects / Q000502:physiology*; D010062:Ovulation Induction / Q000379:methods / Q000662:veterinary*; D049590:Postpartum Period; D011374:Progesterone / Q000008:administration & dosage / Q000097:blood / Q000502:physiology*; D011863:Radioimmunoassay / Q000662:veterinary; D011897:Random Allocation; D014463:Ultrasonography
D007987:Gonadotropin-Releasing Hormone; D011374:Progesterone; D004958:Estradiol; D015237:Dinoprost