[A comparison of the forms of chronic lympholeukemia in relation to the mutational status of the genes of the immunoglobulin variable region].
OBJECTIVE
To compare forms of chronic lymphoid leukemia (CLL) regarding mutational status of immunoglobulin variable genes.


METHODS
We have compared clinical, prognostic and immunophenotypic data obtained on 25 cases with different mutational status of IgV genes. There were 10 patients in the mutated group (median age 49.2 years, male to female ratio = 7:3), and 15 patients in unmutated group (median age 46.5, M:F = 13:2).


RESULTS
Statistically significant differences were noted in overall survival and CD38 expression. 5-year overall survival in unmutated group was 35%, in mutated group 80% (p = 0.07). In unmutated group CD38 was expressed on more than 50% of cells in 7 out of 14 patients, while in the mutated group in 0 of 8 patients (p = 0.007). We noted high frequency of VH1-69 gene usage in unmutated group (7 of 15 patients), while in mutated group it was used in only 1 case of 10.


CONCLUSIONS
We confirm the differences between groups of CLL with different mutational status of IgV genes. Highly restricted usage of VH-genes and CD38 expression possibly suggest that unmutated group also arises from antigen driven cells.
Nikitin
E A
EA
Pivnik
A V
AV
Sudarikov
A B
AB
Valova
G M
GM
Gabeeva
N G
NG
Samoĭlova
R S
RS
Ivanov
D V
DV
Melikian
A L
AL
Kovaleva
L G
LG
D000328:Adult; D000368:Aged; D001483:Base Sequence; D005260:Female; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D016130:Immunophenotyping / Q000379:methods; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics* / Q000401:mortality; D008297:Male; D008875:Middle Aged; D009154:Mutation / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011379:Prognosis; D012313:RNA / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics
D007135:Immunoglobulin Variable Region; D012313:RNA