[Development of a quantitative PCR variant, and use of it for assessing gene expression].
The data on the development of a quantitative PCR technique are presented, including the synthesis of modified primers, the choice of conditions for the removal of reaction-mixture components from the amplified DNA, and the colorimetric detection of the PCR product. The application of this technique to the assessment of the level of gene expression in nonculturable bacterial forms characterized by a drastically reduced metabolism is described.
Aliapkina
Iu S
IuS
Gamaleya Scientific Research Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences, Moscow, Russia.
Romanova
Iu M
IuM
Alekseeva
N V
NV
Kovalev
Iu N
IuN
Gaĭntseva
A V
AV
Gintsburg
A L
AL
D003124:Colorimetry; D020869:Gene Expression Profiling; D016133:Polymerase Chain Reaction / Q000379:methods*