Recombinant bovine interleukin-1beta amplifies the effects of partially purified Pasteurella haemolytica leukotoxin on bovine neutrophils in a beta(2)-integrin-dependent manner.
The influx and death of polymorphonuclear leukocytes within the infected lung are hallmarks of bovine pasteurellosis. Recent reports have shown that the Pasteurella haemolytica leukotoxin (LKT) and other RTX toxins bind beta(2)-integrins on target cells. In this study we demonstrate that exposure of bovine neutrophils to recombinant bovine interleukin-1beta upregulates beta(2)-integrins (CD11a/CD18), which in turn enhance the binding and amplify the biological effects of partially purified LKT on these cells. LKT binding and cytotoxicity were inhibited by addition of an anti-integrin antibody (CD11a/CD18). These findings help to clarify the early events that occur in bovine pasteurellosis and support the hypothesis that inflammatory mediators might increase the severity of pasteurellosis by causing upregulation of beta(2)-integrins that serve as an LKT receptor on bovine neutrophils.
Leite
F
F
Department of Pathobiological Sciences, University of Wisconsin School of Veterinary Medicine, Madison, Wisconsin 53706, USA.
Brown
J F
JF
Sylte
M J
MJ
Briggs
R E
RE
Czuprynski
C J
CJ
D000818:Animals; D018821:CD18 Antigens / Q000378:metabolism*; D002417:Cattle; D005098:Exotoxins / Q000378:metabolism / Q000494:pharmacology* / Q000633:toxicity; D007375:Interleukin-1 / Q000235:genetics / Q000494:pharmacology*; D016978:Mannheimia haemolytica / Q000378:metabolism / Q000472:pathogenicity*; D009504:Neutrophils / Q000378:metabolism*; D012766:Pasteurellosis, Pneumonic / Q000382:microbiology; D011994:Recombinant Proteins / Q000494:pharmacology; D014774:Virulence
D018821:CD18 Antigens; D005098:Exotoxins; D007375:Interleukin-1; D011994:Recombinant Proteins; C018579:leukotoxin