title: [Bacterial infection in the conjunctiva of patients with adenoviral conjunctivitis].
abstract: OBJECTIVE
  We evaluate the microbiological features of mixed infection in adenovirus-infected conjunctiva.
  
  
  METHODS
  Isolation of bacteria was performed in 82 samples of adenoviral conjunctivitis at six eye clinics in Japan.
  
  
  METHODS
  For microbiological diagnosis, we performed immunochromatography (IC) and polymerase chain reaction--restriction fragment length polymorphism (PCR-RFLP) analysis for detection and serotyping of adenovirus, and PCR for detection of herpes simplex virus (HSV) and Chlamydia trachomatis antigens out of 82 samples with adenoviral conjunctivitis.
  
  
  RESULTS
  Pathogenic bacteria were isolated in 6 samples out of 82. Out of these 6 cases, 5 samples were gram-negative rods and most of them were Flavobacterium meningosepticum (4 samples). Adenovirus type 8 was isolated from all these mixed infection cases. However, HSV-1 and Chlamydia trachomatis were not found.
  
  
  CONCLUSIONS
  From these results, it is suggested that gram-negative rods, especially F. meningosepticum, are the most common bacteria causing mixed bacterial infection in adenoviral conjunctivitis.
authors.affiliation: Fujioka Eye Hospital, Yokohama City University School of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Watanabe
authors.forename: E
authors.initials: E
authors.lastname: Uchio
authors.forename: N
authors.initials: N
authors.lastname: Itoh
authors.forename: N
authors.initials: N
authors.lastname: Usui
authors.forename: H
authors.initials: H
authors.lastname: Nakagawa
authors.forename: K
authors.initials: K
authors.lastname: Inoue
authors.forename: R
authors.initials: R
authors.lastname: Hinokuma
authors.forename: Y
authors.initials: Y
authors.lastname: Asato
authors.forename: K
authors.initials: K
authors.lastname: Aoki
authors.forename: S
authors.initials: S
authors.lastname: Ohno
mesh_terms: D000258:Adenovirus Infections, Human / Q000150:complications*; D002690:Chlamydia Infections / Q000150:complications; D002692:Chlamydia trachomatis; D003231:Conjunctivitis / Q000150:complications*; D005417:Flavobacterium; D016905:Gram-Negative Bacterial Infections / Q000150:complications*; D006801:Humans