Expression of E1 component of human branched-chain alpha-keto acid dehydrogenase complex in Escherichia coli by cotransformation with chaperonins GroEL and GroES.
Wynn
R M
RM
Department of Internal Medicine, University of Texas, Southwestern Medical Center, Dallas 75390-8889, USA.
Davie
J R
JR
Song
J L
JL
Chuang
J L
JL
Chuang
D T
DT
D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide); D002460:Cell Line; D018835:Chaperonin 10 / Q000378:metabolism*; D018834:Chaperonin 60 / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D006801:Humans; D007658:Ketone Oxidoreductases / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000378:metabolism*; D010957:Plasmids
D018835:Chaperonin 10; D018834:Chaperonin 60; D009097:Multienzyme Complexes; D007658:Ketone Oxidoreductases; D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)