Acetaldehyde-stimulated PKC activity in airway epithelial cells treated with smoke extract from normal and smokeless cigarettes.
Previously, we have found that acetaldehyde, a volatile component of cigarette smoke, stimulates the protein kinase C (PKC) pathway and inhibits ciliary motility. A "smokeless" cigarette (Eclipse) now exists in which most of the tobacco is not burned, reducing the pyrolyzed components in the extract. We hypothesized that acetaldehyde is a component of cigarette smoke that activates PKC in the airway epithelial cell, and therefore the Eclipse cigarette would not activate epithelial cell PKC. In this study, bovine bronchial epithelial cells (BBEC) were incubated with cigarette smoke extract (CSE) or Eclipse smoke extract (ESE). We found that PKC activity was significantly higher in cells exposed to 5% CSE than cells exposed to 5% ESE or media. When acetaldehyde levels of both extracts were measured by gas chromatography, CSE was found to have 15-20 times greater concentration (microM) of acetaldehyde than ESE. When BBEC were treated with 5% CSE, ciliary beating was further decreased from baseline levels. This decrease in ciliary beating was not observed in cells treated with ESE, suggesting that acetaldehyde contained in CSE slows cilia. These results suggest that volatile components such as acetaldehyde in cigarette smoke may inhibit ciliary motility via a PKC-dependent mechanism.
Wyatt
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Veterans Affairs Medical Center Research Services and Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Nebraska Medical Center, Omaha, Nebraska 68198-5300, USA. twyatt@unmc.edu
Schmidt
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Rennard
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Tuma
D J
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Sisson
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D000818:Animals; D002417:Cattle; D000079:Acetaldehyde / Q000032:analysis / Q000494:pharmacology; D001980:Bronchi / Q000201:enzymology / Q000648:ultrastructure; D002849:Chromatography, Gas; D002923:Cilia / Q000187:drug effects / Q000502:physiology; D004789:Enzyme Activation / Q000187:drug effects; D004847:Epithelial Cells / Q000201:enzymology; D011493:Protein Kinase C / Q000378:metabolism; D012906:Smoke / Q000032:analysis; D062789:Tobacco Products; D014030:Tobacco, Smokeless
D000079:Acetaldehyde; D011493:Protein Kinase C; D012906:Smoke