Mapping of a new target region of allelic loss at 21q22 in primary gastric cancers.
To determine the minimal region of deletion on 21q22 in gastric cancer, we performed a high-density loss of heterozygosity (LOH) study with eight polymorphic microsatellite markers. Among the 43 tumors examined, 20 (50%) of 40 informative carcinomas showed LOH at one or more loci. The peak LOH frequency was identified at D21S1820 (34.2%) in 21q22.3. This data suggests that this locus might harbor a new tumor suppressor gene in an area <0.332 Mb in physical map distance defined by D21S1820 and D21S49. Thus, we speculate that trefoil factor family 1 (TFF1), located in this narrow region, might be the most probable candidate gene involved in gastric cancer carcinogenesis.
Park
W S
WS
Department of Pathology and Genetic Oncology Laboratory, College of Medicine, The Catholic University of Korea, 137-701, Seoul, South Korea.
Oh
R R
RR
Park
J Y
JY
Yoo
N J
NJ
Lee
S H
SH
Shin
M S
MS
Kim
S Y
SY
Kim
Y S
YS
Lee
J H
JH
Kim
H S
HS
An
W G
WG
Lee
J Y
JY
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002891:Chromosomes, Human, Pair 21 / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D006801:Humans; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
D004273:DNA, Neoplasm