title: Human Dengue antibodies against structural and nonstructural proteins.
abstract: Antibodies against dengue virus type 2 and 4 proteins in acute-phase sera of 10 primary and 10 secondary dengue fever and dengue hemorrhagic fever patients were studied by Western blotting. In the first group the immune response was barely detectable, while in the second group more proteins were detected, with a very strong reaction. Anti-NS1 and -NS3 antibodies were detected mainly in secondary cases. Anti-E, -NS3, and -NS5 antibodies were detected in a high number of cases. The possibility of implementing early diagnostic assays for antigen detection is suggested.
authors.affiliation: Department of Virology, PAHO/WHO Collaborating Center for Viral Diseases, "Pedro Kouri" Tropical Medicine Institute, Autopista Novia del Mediodía, Marianao 13, Havana, Cuba.
authors.forename: K
authors.initials: K
authors.lastname: Valdés
authors.forename: M
authors.initials: M
authors.lastname: Alvarez
authors.forename: M
authors.initials: M
authors.lastname: Pupo
authors.forename: S
authors.initials: S
authors.lastname: Vázquez
authors.forename: R
authors.initials: R
authors.lastname: Rodríguez
authors.forename: M G
authors.initials: MG
authors.lastname: Guzmán
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D015153:Blotting, Western / Q000379:methods; D002522:Chlorocebus aethiops; D003715:Dengue / Q000097:blood / Q000276:immunology*; D003716:Dengue Virus / Q000276:immunology; D006801:Humans; D019595:Severe Dengue / Q000097:blood / Q000276:immunology*; D014709:Vero Cells; D017361:Viral Nonstructural Proteins / Q000276:immunology*; D015678:Viral Structural Proteins / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D017361:Viral Nonstructural Proteins; D015678:Viral Structural Proteins