Creutzfeldt-Jakob disease and oxidative stress.
OBJECTIVE
Substantial evidence supports the hypothesis that oxygen free radicals are involved in various neurodegenerative disorders. To assess the presence of oxidative stress in Creutzfeldt-Jakob disease (CJD) we examined the concentrations of malondialdehyde (MDA) as an established marker of lipid peroxidation.


METHODS
MDA was quantified by high performance liquid chromatography (HPLC) in cerebrospinal fluid (CSF; n=12) and in serum (n=11) samples of CJD patients and healthy controls (n = 15).


RESULTS
Mean values in healthy controls: 2.56 nmol/ml +/- 0.46 (CSF) and 1.94 nmol/ ml +/- 0.67 (serum); mean values in CJD patients: 2.64 nmol/ml +/- 0.67 (CSF) and 1.68 nmol/ml +/- 0.79 (serum). No significant (P>0.05) difference between CJD patients and controls was observed.


CONCLUSIONS
The results indicated that the CSF and serum of CJD patients showed no higher endogenous levels of MDA as compared to normal healthy controls. These findings provide no evidence for an additional role of oxidative stress in the pathogenetic mechanism underlying CJD neurodegeneration.
Bleich
S
S
Department of Psychiatry, Georg-August-University of Göttingen, Germany.
Kropp
S
S
Degner
D
D
Zerr
I
I
Pilz
J
J
Gleiter
C H
CH
Otto
M
M
Rüther
E
E
Kretzschmar
H A
HA
Wiltfang
J
J
Kornhuber
J
J
Poser
S
S
D015415:Biomarkers / Q000032:analysis; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid; D007562:Creutzfeldt-Jakob Syndrome / Q000503:physiopathology*; D006801:Humans; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000097:blood / Q000134:cerebrospinal fluid; D018384:Oxidative Stress
D015415:Biomarkers; D008315:Malondialdehyde