Molecular cloning and biological characterization of a novel murine lymphoid growth factor.
Using a bioassay consisting of the proliferation of a murine B cell line, a cDNA of a gene whose product supports the growth of that cell line was isolated from a thymic stromal cell line. This factor, termed thymic stromal lymphopoietin (TSLP), is a protein of 140 amino acids. The gene encoding TSLP was mapped to murine chromosome 18. Purified recombinant TSLP supported the growth of pre-B cell colonies in vitro, but had no myelopoietic activity. TSLP had comitogenic activity for fetal thymocytes, but was not as potent as interleukin 7 in lobe submersion cultures. Injection of TSLP into neonatal mice induced the expansion of B220(+)BP-1(+) pre-B cells.
Sims
J E
JE
Department of Molecular Biology, Immunex Corporation, Seattle, Washington 98101, USA. sims@immunex.com
Williams
D E
DE
Morrissey
P J
PJ
Garka
K
K
Foxworthe
D
D
Price
V
V
Friend
S L
SL
Farr
A
A
Bedell
M A
MA
Jenkins
N A
NA
Copeland
N G
NG
Grabstein
K
K
Paxton
R J
RJ
D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects*; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D016207:Cytokines / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*; D018076:DNA, Complementary / Q000302:isolation & purification; D005260:Female; D006410:Hematopoiesis / Q000187:drug effects*; D015851:Interleukin-7 / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction
D016207:Cytokines; D018076:DNA, Complementary; D015851:Interleukin-7; D012333:RNA, Messenger; D011994:Recombinant Proteins