title: Evolution of an Alu DNA element of type Sx in the lineage of primates and the origin of an associated tetranucleotide microsatellite.
abstract: A 394-bp DNA fragment, which in human is on chromosome 6 near the MOG (myelin oligodendrocyte glycoprotein) gene and encompasses an Alu element and an associated tetranucleotide microsatellite, was sequenced from a large range of primate species to follow its evolutionary divergence and to understand the origin of the microsatellite. This Alu element is found at the same orthologous position in all primates sequenced, but the tetranucleotide repeat is present only in Catarrhini between the 3'-oligo(dA) of the Alu element and the 3' flanking direct repeat. Little intraspecific variation was found. Sequence identity values for this orthologous primate Alu averaged 90% (82-99%) with transitions comprising between 70% and 100% of the observed nucleotide substitutions. Although the insertion of the Alu element predates the separation of these species, the original sequence of the site of integration can still be identified. This identification of the direct repeats suggests an active role of the oligo(dA) of the Alu element in the origin of the tetranucleotide repeats. The microsatellite probably appeared after the insertion of the Alu element, early in the lineage leading to the common ancestor of the hominoids and the Old World monkeys.
authors.affiliation: Génétique des populations, CESAC, UMR C5576, Toulouse, France. bcrouau@cict.fr
authors.forename: B
authors.initials: B
authors.lastname: Crouau-Roy
authors.forename: I
authors.initials: I
authors.lastname: Clisson
mesh_terms: D020087:Alu Elements; D000818:Animals; D001483:Base Sequence; D002144:Callithrix; D002896:Chromosomes, Human, Pair 6; D019143:Evolution, Molecular; D014644:Genetic Variation; D006071:Gorilla gorilla; D000882:Haplorhini; D006801:Humans; D006922:Hylobates; D008251:Macaca; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D002679:Pan troglodytes; D010215:Papio; D010802:Phylogeny; D011148:Pongo pygmaeus; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity