Separation of phosphatidylinositol 4-phosphate from a mixture with phosphatidylserine and phosphatidylinositol by Sep-Pak C18 cartridge.
A simple and efficient method for the separation of phosphatidylinositol 4-phosphate (PI 4-P) from phosphatidylinositol (PI) and phosphatidylserine (PS) is described. A mixture of PI, PI 4-P and PS was injected onto a Sep-Pak C18 cartridge. PI and PS were flushed through the cartridge with solvent 1 [methanol-chloroform (3: 1)] while PI 4-P remained in it. Then the cartridge was inverted, and PI 4-P was eluted backward with solvent 2 [chloroform-methanol-0.5 M aqueous ammonium hydroxide (9:7:2)].
Morisaki
N
N
Institute of Molecular and Cellular Biosciences, The University of Tokyo, Japan.
Masaoka
M
M
Shirai
R
R
Hashimoto
Y
Y
D018129:Phosphatidylinositol Phosphates / Q000302:isolation & purification*; D010716:Phosphatidylinositols / Q000032:analysis*; D010718:Phosphatidylserines / Q000032:analysis*
D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols; D010718:Phosphatidylserines; C037178:phosphatidylinositol 4-phosphate