Acute effects of smoke exposure on the cellular and cytokine profile in isolated perfused lungs.
The aim of this study was to assess the acute effects of cigarette smoke exposure on cellular and cytokine profile in BAL fluids in an isolated perfused rabbit assay. The experimental animals were categorized into four groups: (1) unexposed controls and (2) cigarette smoke-exposed animals perfused with autologous whole blood; (3) unexposed controls and cigarette smoke-exposed; (4) cigarette smoke-exposed animals perfused with Krebs' Ringer solution containing 5% bovine serum albumin and glucose. Cigarette smoke induced an increase in total cell numbers (mainly alveolar macrophages in BAL fluids) and an increase in the permeability index of BAL. Levels of interleukin 8 were also significantly decreased in BAL fluids due to acute effects of cigarette smoke exposure. The most likely explanation for cigarette smoke-induced increase of inflammatory cells in BAL in lungs is because of the release of pre-existing cells from reservoirs within the lungs. The acute effects of cigarette smoke-induced increase of pulmonary epithelial permeability may also play an important role in the cellular recruitment into airspaces from the lung reservoirs.
Kyi
M
M
The Pulmonary Medicine, Tokyo Medical and Dental University, 5-45, Yushima 1-chome, Bunkyo-Ku, 113-8519, Tokyo, Japan.
Miyazaki
Y
Y
Inoue
T
T
Miyake
S
S
Matsukawa
A
A
Yoshizawa
Y
Y
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002452:Cell Count; D016207:Cytokines / Q000378:metabolism*; D066298:In Vitro Techniques; D016209:Interleukin-8 / Q000378:metabolism; D008168:Lung / Q000378:metabolism* / Q000473:pathology*; D010477:Perfusion; D010539:Permeability; D011817:Rabbits; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*
D016207:Cytokines; D016209:Interleukin-8; D014028:Tobacco Smoke Pollution