Upregulation of intercellular adhesion molecule-1 (ICAM-1) on rat Schwann cells in vitro: comparison of interferon-gamma, tumor necrosis factor-alpha and interleukin-1.
We investigated the expression and upregulation of intercellular adhesion molecule-1 (ICAM-1) in cultures of neonatal rat Schwann cells. Unstimulated cells expressed little or no ICAM-1 as determined by binding of monoclonal antibody to rat ICAM-1 using qualitative and quantitative immunofluorescence. ICAM-1 positive cells when present represented a very small minority (<4%) of Schwann cells. Incubation with recombinant rat interferon-gamma, recombinant rat IL-1beta, recombinant rat tumor necrosis factor-alpha, unfractionated rat cytokines but not purified porcine transforming growth factor-beta or recombinant rat IL-2, resulted in upregulation of ICAM-1 on Schwann cells. Different kinetics were noted for ICAM-1 upregulation by the different recombinant and purified cytokines. The upregulation of ICAM-1 on Schwann cells may be important in inflammatory diseases of the peripheral nervous system as well as in myelination and remyelination.
Lisak
R P
RP
Department of Neurology, Wayne State University School of Medicine, Detroit Medical Center, MI, USA.
Bealmear
B
B
D000818:Animals; D000831:Animals, Newborn; D005347:Fibroblasts; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D007371:Interferon-gamma / Q000494:pharmacology*; D007375:Interleukin-1 / Q000494:pharmacology*; D009186:Myelin Sheath / Q000187:drug effects / Q000648:ultrastructure; D009836:Oligodendroglia / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000494:pharmacology; D012583:Schwann Cells / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D012584:Sciatic Nerve / Q000166:cytology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D015854:Up-Regulation / Q000187:drug effects*
D007375:Interleukin-1; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma