Biochemical properties of the fibrinogen component of a fibrin glue before and after severe dry heat treatment.
Functional biochemical properties of 5 batches of the fibrinogen component of a fibrin glue produced by the ZLB Central Laboratory, Bern, each consisting of 4 different in-process samples (taken after the first and second precipitation step, lyophilization, and dry-heat treatment) were studied in vitro. We focused our attention on the effect of the anti-viral treatment of the lyophilized product by dry heat for 1 h at 100 degrees C. A slight reduction in maximal turbidity of all heat-treated samples was observed during the clotting assay compared to nontreated samples. Treatment with dry heat did not result in generation of fibrinogen fragments that might accelerate tissue-plasminogen-activator (t-PA)-enhanced plasminogen to plasmin conversion. The time course of fibrin cross-linking by factor XIII showed no differences between heated and unheated samples. This result indicates that exposure of the fibrinogen component to severe heat neither reduced activity of factor XIIIa nor affected the correct alignment of cross-linking sites in polymerized fibrin. Incubation of fibrinogen with thrombin, plasminogen, and t-PA resulted in a slightly enhanced degradation of fibrin derived from the heat-treated samples. The amount of residual moisture, determined to be within the range of 0.6-2.1% before heat treatment, did not influence clotting, cross-linking, and fibrinolysis parameters. In conclusion, the virus inactivation treatment by dry heat for 1 h at 100 degrees C induces no significant alterations of the in vitro biochemical properties of the fibrinogen component of this fibrin glue.
Bolliger-Stucki
B
B
Central Hematology Laboratory, Inselspital, University Hospital, Bern, Switzerland. bettina_bolliger@med.unc.edu
Baillod
P
P
Mäder
W
W
Furlan
M
M
D003432:Cross-Linking Reagents; D015718:Fibrin Tissue Adhesive / Q000737:chemistry* / Q000378:metabolism*; D005340:Fibrinogen / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D005341:Fibrinolysin / Q000378:metabolism; D005612:Freeze Drying; D006358:Hot Temperature; D007700:Kinetics; D009391:Nephelometry and Turbidimetry; D013816:Thermodynamics
D003432:Cross-Linking Reagents; D015718:Fibrin Tissue Adhesive; D005340:Fibrinogen; D005341:Fibrinolysin