Tricistronic and tetracistronic retroviral vectors for gene transfer.
We have combined the picornavirus foot-and-mouth disease virus (FMDV) 2A sequence and the internal ribosome entry sites (IRESes) from encephalomyocarditis virus (ECMV) and avian reticuloendotheliosis virus type A (REV-A) to construct tricistronic and tetracistronic vectors. All the polycistronic constructs show high titers and expression of the genes inserted. Clones have been obtained in which cells simultaneously express the three or four genes carried by the polycistronic vectors.
De Felipe
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Departamento de Bioquímica y Biología Molecular-Centro de Biología Molecular Severo Ochoa, Facultad de Ciencias, Universidad Autónoma de Madrid, Cantoblanco 28049 Madrid, Spain.
Izquierdo
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D016475:3T3 Cells / Q000821:virology; D000818:Animals; D005537:Aphthovirus / Q000235:genetics; D002999:Clone Cells / Q000821:virology; D004686:Encephalomyocarditis virus / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics*; D051379:Mice; D012045:Regulatory Sequences, Nucleic Acid; D001356:Reticuloendotheliosis virus / Q000235:genetics; D012190:Retroviridae / Q000235:genetics*; D012270:Ribosomes / Q000235:genetics