Differential expression of nicotinamide adenine dinucleotide phosphate-diaphorase in hypothalamic areas of obese Zucker rats.
Several studies have suggested that the activity of nitric oxide synthase (NOS) may be involved in the regulation of food intake in the genetically obese Zucker rats. In the present study, we investigated the expression of NOS in various hypothalamic regions of obese and lean Zucker rats using nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase histochemistry. Obese Zucker rats showed significantly lower staining intensities of NADPH-diaphorase-positive neurons in the paraventricular nucleus (PVN), lateral hypothalamic area (LHA) and ventromedial hypothalamic nucleus (VMH) than lean Zucker rats did. The differences in staining intensities between obese and lean Zucker rats were large in both the PVN and LHA, but such differences were relatively small in the VMH.
Kim
M J
MJ
Kohwang Medical Research Institute, College of Medicine, Kyung Hee University, 1 Hoegi-Dong, Tongdaemoon-Ku, 130-701, Seoul, South Korea.
Kim
Y
Y
Choe
B K
BK
Kim
S A
SA
Lee
H J
HJ
Kim
J W
JW
Huh
Y
Y
Kim
C
C
Chung
J H
JH
D000818:Animals; D008058:Dihydrolipoamide Dehydrogenase / Q000096:biosynthesis*; D005260:Female; D006651:Histocytochemistry; D007026:Hypothalamic Area, Lateral / Q000201:enzymology / Q000473:pathology; D007031:Hypothalamus / Q000201:enzymology* / Q000473:pathology; D009474:Neurons / Q000201:enzymology / Q000473:pathology; D019001:Nitric Oxide Synthase / Q000378:metabolism; D009765:Obesity / Q000201:enzymology* / Q000473:pathology; D010286:Paraventricular Hypothalamic Nucleus / Q000201:enzymology / Q000473:pathology; D051381:Rats; D011922:Rats, Mutant Strains; D011924:Rats, Zucker; D013194:Staining and Labeling; D014697:Ventromedial Hypothalamic Nucleus / Q000201:enzymology / Q000473:pathology
D019001:Nitric Oxide Synthase; D008058:Dihydrolipoamide Dehydrogenase