Sensitive high-performance thin-layer chromatographic method for the estimation of diospyrin, a tumour inhibitory agent from the stem bark of Diospyros montana Roxb.
Diospyrin, a tumour inhibitory agent from the stem bark of Diospyros montana was isolated and characterised. A sensitive high-performance thin-layer chromatographic (HPTLC) method was developed for the estimation of diospyrin. The method was validated for precision (intra- and inter-day), repeatability and accuracy. The method was found to be precise, with the RSDs for intra-day in the range of 0.72-1.85% and RSDs for inter-day in the range of 1.06-2.95%, for different concentrations. Instrumental precision and repeatability of the method were found to be 0.086 and 0.937 (% CV), respectively. Accuracy of the method was checked by performing the recovery study at two levels and average percentage recovery was found to be 97.87%. The developed HPTLC method was adopted for the estimation of diospyrin content of the stem bark of D. montana from different regions, which varied from 0.35 to 0.47% (w/w) in the samples.
Ravishankara
M N
MN
BV Patel Pharmaceutical Education and Research Development Centre, Thaltej, Ahmedabad, India.
Shrivastava
N
N
Jayathirtha
M G
MG
Padh
H
H
Rajani
M
M
D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis*; D002855:Chromatography, Thin Layer / Q000379:methods*; D019684:Magnoliopsida / Q000737:chemistry*; D009285:Naphthoquinones / Q000032:analysis*; D018547:Plant Stems / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D000972:Antineoplastic Agents, Phytogenic; D009285:Naphthoquinones; C043546:diospyrin