Epidemiological relationships between Aeromonas strains isolated from symptomatic children and household environments as determined by ribotyping.
Ribotyping was used to study the epidemiology of Aeromonas associated gastro-enteritis in young children. Ribotyping patterns of 29 Aeromonas strains (16 Aeromonas caviae, 8 Aeromonas hydrophila, 3 Aeromonas eucrenophila, 1 Aeromonas veronii, and 1 Aeromonas encheleia) isolated from primary stool cultures of sick children were compared using the GelCompare software with patterns of 104 strains (39 Aeromonas eucrenophila, 29 Aeromonas caviae, 11 Aeromonas encheleia, 10 Aeromonas hydrophila, 6 Aeromonas bestiarum, 3 Aeromonas veronii, 3 Aeromonas popoffii and 3 Aeromonas media) isolated from their household environment in order to investigate the route of transmission of these bacteria. Fifteen strains (approximately 47%) isolated from stool cultures of patients showed the same riboprofile as strains found in contacts or environment. In particular, three strains isolated from patients shared the same riboprofile with strains found in their domestic environment. The wide diffusion of potentially pathogenic Aeromonas strains in our household samples, and the high rate of asymptomatic carriers among family members, suggested that predisposing factors of the host could make children prone to an Aeromonas-related intestinal disease.
Demarta
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A
Istituto Cantonale Batteriosierologico, Laboratory of Microbial Ecology, University of Geneva, Lugano, Switzerland. antonella.demarta@ti.ch
Tonolla
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M
Caminada
A
A
Beretta
M
M
Peduzzi
R
R
D000333:Aeromonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016980:Aeromonas hydrophila / Q000302:isolation & purification; D000367:Age Factors; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis; D003967:Diarrhea / Q000453:epidemiology / Q000209:etiology / Q000382:microbiology; D003968:Diarrhea, Infantile / Q000453:epidemiology / Q000209:etiology / Q000382:microbiology; D004587:Electrophoresis, Agar Gel; D004751:Enteritis / Q000453:epidemiology / Q000209:etiology / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D005260:Female; D005759:Gastroenteritis / Q000453:epidemiology / Q000209:etiology / Q000382:microbiology; D005767:Gastrointestinal Diseases / Q000453:epidemiology* / Q000209:etiology / Q000382:microbiology; D016905:Gram-Negative Bacterial Infections / Q000453:epidemiology*; D006801:Humans; D007223:Infant; D008297:Male; D012329:RNA, Bacterial / Q000032:analysis; D021521:Ribotyping; D012737:Sex Factors
D004269:DNA, Bacterial; D012329:RNA, Bacterial