Stabilization of D-amino-acid oxidase from Trigonopsis variabilis by manganese dioxide.
Stabilization of immobilized D-amino-acid oxidase was achieved as follows. Yeast Trigonopsis variabilis producing D-amino-acid oxidase was used to deaminate cephalosporin C to glutaryl-7-aminocephalosporanic acid. Permeabilized cells were co-immobilized with manganese dioxide by entrapment in (poly)acrylamide gel so that hydrogen peroxide, liberated in the reaction, could be partially deactivated and both the enzyme and the substrate could be stabilized. Activity of entrapped cells was determined by HPLC and enzyme flow microcalorimetry. The process was evaluated in terms of activity, immobilization yield, storage stability and oxo-product formation by immobilized preparations. The storage stability of immobilized biocatalysts with MnO2 was nearly doubled and production of 2-oxoadipyl-7-aminocephalosporanic acid was 2-3-fold higher than by entrapped cells without MnO2. Glutaryl-7-aminocephalosporanic acid can be easily obtained from the resulting oxo-product by a non-enzymic reaction via externally added hydrogen peroxide.
Vikartovská-Welwardová
A
A
Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia.
Michalková
E
E
Gemeiner
P
P
Welward
L
L
D001203:Ascomycota / Q000201:enzymology*; D002151:Calorimetry; D002511:Cephalosporins / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003605:D-Amino-Acid Oxidase / Q000378:metabolism*; D004795:Enzyme Stability / Q000187:drug effects; D004800:Enzymes, Immobilized; D017895:Manganese Compounds / Q000494:pharmacology; D010087:Oxides / Q000494:pharmacology
D002511:Cephalosporins; D004800:Enzymes, Immobilized; D017895:Manganese Compounds; D010087:Oxides; C025163:cephalosporin C; C027424:manganese oxide; D003605:D-Amino-Acid Oxidase