title: Multiple-site optical recording of mouse brainstem evoked by vestibulocochlear nerve stimulation.
abstract: We used optical imaging to investigate the mouse cochlear and vestibular nucleus in brainstem slices using a voltage-sensitive dye, RH 155. As a result, the spatiotemporal patterns of excitatory propagation were shown. These optical signals consisted of two components consisting of a spike-like fast signal and a long-lasting slow signal. All responses were abolished by tetrodotoxin. The slow signals were eliminated under a Ca(2+)-free solution. In addition, synaptic fatigue was also observed. The present study indicated the feasibility of optical recording for visually revealing the synaptic transmission in both the vestibular and cochlear nucleus.
authors.affiliation: Department of Otolaryngology, Kansai Medical University, Fumizono-cho 10-15, Moriguchi, 570-8507, Osaka, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Yang
authors.forename: T
authors.initials: T
authors.lastname: Doi
authors.forename: M
authors.initials: M
authors.lastname: Asako
authors.forename: A
authors.initials: A
authors.lastname: Matsumoto-Ono
authors.forename: T
authors.initials: T
authors.lastname: Kaneko
authors.forename: T
authors.initials: T
authors.lastname: Yamashita
mesh_terms: D000818:Animals; D001933:Brain Stem / Q000187:drug effects / Q000502:physiology*; D004558:Electric Stimulation; D051379:Mice; D008813:Mice, Inbred ICR; D055095:Optics and Photonics; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology*; D013779:Tetrodotoxin / Q000494:pharmacology; D000159:Vestibulocochlear Nerve / Q000187:drug effects / Q000502:physiology*
chemical_list: D013779:Tetrodotoxin