Protection of the flowers of Prunus persica extract from ultraviolet B-induced damage of normal human keratinocytes.
For an attempt to develop safe materials protecting solar ultraviolet (UV)-induced skin damage, plant extracts were evaluated for their inhibitory activities of free radical generation and arachidonic acid/metabolites release from UVB-irradiated normal human keratinocytes. From the results of these screening procedures, the ethanol extract of the flowers of Prunus persica (Ku-35) was selected for further study. It was found that Ku-35 (100-1,000 microg/ml) inhibited the amount of 14C-arachidonic acid/metabolites release from UVB-irradiated keratinocytes. It was also demonstrated that Ku-35 possessed the protective activity against UV-induced cytotoxicity of keratinocytes and fibroblasts. In addition, Ku-35 was revealed to protect UVB-induced erythema formation using guinea pigs in preliminary in vivo study. All these results indicate that the flowers of P. persica extract may be beneficial for protecting UV-induced skin damage when topically applied.
Kim
Y H
YH
College of Pharmacy, Kangwon National University, Chunchon, Korea.
Yang
H E
HE
Kim
J H
JH
Heo
M Y
MY
Kim
H P
HP
D016475:3T3 Cells; D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D015603:Keratinocytes / Q000528:radiation effects*; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology; D010946:Plants, Medicinal; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D014466:Ultraviolet Rays / Q000009:adverse effects*
D010936:Plant Extracts; D011837:Radiation-Protective Agents; D016718:Arachidonic Acid