18F-FDG labelling of human leukocytes.
Radiolabelled leukocytes are useful for the imaging of inflammation and infection, and 18F-fluorodeoxyglucose (18F-FDG) is known to concentrate in metabolically active cells. We evaluated the feasibility of leukocyte labelling with 18F-FDG using ACD and heparin anticoagulants at 20 degrees C and 37 degrees C, with and without gentle mixing during incubation. With leukocytes (WBC) harvested from 20 ml blood, studies were performed using 18F-FDG in concentrations of 3.7-74 MBq (0.1-2.0 mCi). 18F-FDG WBC stability in platelet-poor plasma was assessed at 1-4 h. Satisfactory labelling efficiency was achieved with incubation in heparin-saline at 37 degrees C for 30 min (62.7+/-1.6%), and was further enhanced by mixing during incubation (78.1+/-3.9%). Cell labelling was predominantly of granulocytes (78.5+/-1.4%). 18F-FDG WBC was relatively stable in platelet-poor plasma for up to 4 h, and no cell staining was observed in viability studies using trypan blue. These results indicate the feasibility of leukocyte labelling with 18F-FDG, providing an approach that may be useful in PET imaging of inflammation and infection.
Forstrom
L A
LA
Nuclear Medicine, Department of Diagnostic Radiology, Mayo Clinic, Rochester, MN 55905, USA. forstrom.lee@mayo.edu
Mullan
B P
BP
Hung
J C
JC
Lowe
V J
VJ
Thorson
L M
LM
D000925:Anticoagulants / Q000494:pharmacology; D002470:Cell Survival; D019788:Fluorodeoxyglucose F18; D006801:Humans; D066298:In Vitro Techniques; D007553:Isotope Labeling; D007962:Leukocytes / Q000000981:diagnostic imaging* / Q000187:drug effects; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals; D013696:Temperature
D000925:Anticoagulants; D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18