Identification and characterization of the ribosome-associated protein, HrpA, of Bacillus Calmette-Guérin.
HrpA was found as a ribosome-associated protein which appeared in heat-stressed Mycobacterium bovis Bacillus Calmette-Guérin. Here, we have studied the function of HrpA in vitro. HrpA is a heat shock protein belonging to a small heat shock protein family. The putative molecular mass was 17784.86 kDa. Recombinant HrpA formed large complexes of nonamer or dodecamer. HrpA prevented the aggregation of enzymes under heat shock conditions, and it formed stable complexes with partially denatured enzymes. HrpA was induced temporarily by oxygen repletion after anaerobic condition.
Tabira
Y
Y
Nagasaki University Environmental Protection Center, Bunkyo-machi 1-14, Nagasaki, 852-8521, Japan. tabira@net2.nagasaki-u.ac.jp
Ohara
N
N
Yamada
T
T
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D053487:DEAD-box RNA Helicases; D004798:Enzymes / Q000737:chemistry; D029968:Escherichia coli Proteins; D016680:Genome, Bacterial; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009163:Mycobacterium bovis / Q000378:metabolism*; D010100:Oxygen; D020365:RNA Helicases; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment
D001426:Bacterial Proteins; D004798:Enzymes; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; D012269:Ribosomal Proteins; C092662:hrpA protein, E coli; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases; D010100:Oxygen