Suppression of adhesion and invasion of hepatoma cells in culture by tea compounds through antioxidative activity.
To determine the actions of tea components on the invasion of a rat ascites hepatoma cell line of AH109A and to understand their modes of action, the cancer cells were co-cultured with a rat mesentery-derived mesothelial cell monolayer in the presence of tea components. The synergistic effects of (-)-epicatechin (EC) with (-)-epigallocatechin gallate (EGCG) on AH109A invasion were demonstrated. Further study showed that 10 microM of EGCG or theaflavins, or 2.5 microM of ethylenediaminetetra-acetic (EDTA) entirely abolished the increase in AH109A adhesion and invasion stimulated by reactive oxygen species (ROS) from the hypoxanthine-xanthine oxidase system. Our results suggest that (.)OH(-)- and other ROS-scavenging activity of EGCG and theaflavins may be responsible for the inhibition of (.)OH(-)- and related ROS-potentiated AH109A adhesion and invasion to the cultured rat mesothelial cell monolayer.
Zhang
G
G
Department of Applied Biological Science, Tokyo Noko University, Saiwaicho 3-5-8, Fuchu, Tokyo 183-8509, Japan.
Miura
Y
Y
Yagasaki
K
K
D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D044946:Biflavonoids; D006528:Carcinoma, Hepatocellular / Q000473:pathology / Q000517:prevention & control*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D009361:Neoplasm Invasiveness / Q000517:prevention & control*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013662:Tea / Q000737:chemistry*; D014407:Tumor Cells, Cultured
D000975:Antioxidants; D044946:Biflavonoids; D017382:Reactive Oxygen Species; D013662:Tea; C056068:theaflavin; D002392:Catechin; C045651:epigallocatechin gallate