title: DNA synthesis and nucleolar organizer regions circadian rhythm in mouse regenerating liver hepatocytes.
abstract: DNA synthesis and Nucleolar Organizer Regions (NORs) were studied in C3HS inbred mice standardized for periodicity analysis. Immunohistochemical detection of Bromodeoxyuridine (BrdU) incorporated into DNA with a monoclonal antibody and silver staining of NORs (AgNORs) were assessed by means of a digital image analysis system in histological sections of regenerating liver. Tissue samples were obtained at different times after hepatectomy along a circadian span. The results showed a strong correlation of values between DNA synthesis (BrdU labelling index) and AgNOR numbers, with higher counts during the activity period of animals at 00:00/38 and 04:00/42 hours Time of Day/Hours Post-Hepatectomy (TD/HPH), being the differences with other time points highly significant. Our observations demonstrate the existence of a strong correlation of DNA synthesis measured by BrdU incorporation and AgNOR numbers with a defined circadian rhythm in mouse regenerating hepatocytes.
authors.affiliation: Instituto de Embriología, Biología e Histología, Facultad de Ciencias Médicas, UNLP, La Plata, Argentina. drmartin@netverk.com.ar
authors.forename: C A
authors.initials: CA
authors.lastname: Martín
authors.forename: J M
authors.initials: JM
authors.lastname: Surur
authors.forename: M N
authors.initials: MN
authors.lastname: García
authors.forename: A F
authors.initials: AF
authors.lastname: Badrán
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine; D002940:Circadian Rhythm / Q000502:physiology*; D004247:DNA / Q000096:biosynthesis*; D008099:Liver / Q000166:cytology* / Q000254:growth & development* / Q000378:metabolism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009697:Nucleolus Organizer Region / Q000235:genetics* / Q000378:metabolism*; D012038:Regeneration / Q000502:physiology*; D016622:Silver Staining / Q000379:methods
chemical_list: D004247:DNA; D001973:Bromodeoxyuridine