Evidence for involvement of the PKC-alpha isoform in myogenic contractions of the coronary microcirculation.
The role of protein kinase C (PKC) isoforms in myogenic tone of the ferret coronary microcirculation was investigated by measuring fura 2 Ca(2+) signals, PKC immunoblots, contractile responses, and confocal microscopy of PKC translocation. Phorbol ester-evoked contractions were completely abolished in the absence of extracellular Ca(2+) but involved a Ca(2+) sensitization relative to KCl contractions. Immunoblotting using isoform-specific antibodies showed the presence of PKC-alpha and -iota and traces of PKC-epsilon and -mu in the ferret coronary microcirculation. PKC-beta was not detectable. When intraluminal pressure (40 to 60 and 80 mmHg) was increased, ferret coronary arterioles showed a transient increase in fura 2 Ca(2+) signals, whereas the myogenic tone remained sustained. The increase in Ca(2+) and tone was sustained at 100 mmHg. Isolated ferret coronary arterioles were fixed and immunostained for PKC-alpha at 40 and 100 mmHg intraluminal pressure. PKC translocation was determined by confocal microscopy. Increased PKC translocation was observed when vessels were exposed to 100 mmHg relative to that at resting pressure (40 mmHg). These results suggest a link between the Ca(2+) sensitization that occurs during the myogenic contraction and activation of the alpha-isoform of PKC.
Dessy
C
C
Signal Transduction Group, Boston Biomedical Research Institute, Boston, MA 02114, USA.
Matsuda
N
N
Hulvershorn
J
J
Sougnez
C L
CL
Sellke
F W
FW
Morgan
K G
KG
D000818:Animals; D001160:Arterioles / Q000201:enzymology / Q000502:physiology; D002118:Calcium / Q000378:metabolism; D003326:Coronary Circulation / Q000502:physiology*; D003331:Coronary Vessels / Q000201:enzymology* / Q000502:physiology; D005289:Ferrets; D005456:Fluorescent Dyes; D016257:Fura-2; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000378:metabolism*; D008297:Male; D008833:Microcirculation / Q000201:enzymology / Q000502:physiology; D009119:Muscle Contraction / Q000187:drug effects; D009129:Muscle Tonus / Q000187:drug effects; D009218:Myosins / Q000378:metabolism; D010703:Phorbol Esters / Q000494:pharmacology; D010766:Phosphorylation / Q000187:drug effects; D011189:Potassium Chloride / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism*; D051571:Protein Kinase C-alpha; D015398:Signal Transduction / Q000187:drug effects; D014661:Vasoconstriction / Q000187:drug effects / Q000502:physiology*
D005456:Fluorescent Dyes; D007527:Isoenzymes; D010703:Phorbol Esters; C047907:12-deoxyphorbol-13-isobutyrate-20-acetate; D011189:Potassium Chloride; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha; D009218:Myosins; D002118:Calcium; D016257:Fura-2