Determination of sulfonamides in swine meat by immunoaffinity chromatography.
A procedure was developed for the preparation of anti-sulfonamide (SA) group-specific antibodies and immunosorbents. Sulfonamide haptens and conjugates were synthesized by building spacer arms on an N1 group of 4-aminobenzensulfonamide. The anti-SA group-specific antibodies and immunosorbents were prepared successfully. After extraction with methanol-water (8 + 2), sulfamonomethoxine, sulfadimethoxine, and sulfaquinoxaline were cleaned up on immunoaffinity columns and determined by reversed-phase liquid chromatography with UV detection at 270 nm. The recoveries from fortified swine meat (10-100 microg/kg) ranged from 70.8 to 94.1%, with coefficients of variation of 3.4-12.9%. Limits of detection were 1-2 microg/kg.
Li
J S
JS
China Agricultural University, Department of Veterinary Pharmacology, Pharmacy and Toxicology, College of Veterinary Medicine, Beijing, People's Republic of China.
Li
X W
XW
Yuan
J X
JX
Wang
X
X
Li
J S
JS
Li
X W
XW
Yuan
J X
JX
Wang
X
X
D000818:Animals; D000918:Antibody Specificity; D000941:Antigens / Q000737:chemistry / Q000276:immunology; D002846:Chromatography, Affinity / Q000379:methods*; D006241:Haptens / Q000737:chemistry / Q000276:immunology; D007163:Immunosorbent Techniques; D008460:Meat / Q000032:analysis*; D011786:Quality Control; D012710:Serum Albumin, Bovine; D000077145:Sulfanilamide; D013424:Sulfanilamides / Q000276:immunology; D013449:Sulfonamides / Q000032:analysis* / Q000276:immunology; D013552:Swine
D000941:Antigens; D006241:Haptens; D013424:Sulfanilamides; D013449:Sulfonamides; D000077145:Sulfanilamide; D012710:Serum Albumin, Bovine