title: Bacillus subtilis ccpA gene mutants specifically defective in activation of acetoin biosynthesis.
abstract: A large number of carbon source utilization pathways are repressed in Bacillus subtilis by the global regulator CcpA, which also acts as an activator of carbon excretion pathways during growth in media containing glucose. In this study, CcpA mutants defective in transcriptional activation of the alsSD operon, which is involved in acetoin biosynthesis, were identified. These mutants retained normal glucose repression of amyE, encoding alpha-amylase, and acsA, encoding acetyl-coenzyme A synthetase, and normal activation of ackA, which is involved in acetate excretion; in these ccpA mutants the CcpA functions of activation of the acetate and acetoin excretion pathways appear to be separated.
authors.affiliation: Department of Biochemistry and Molecular Biology, Albany Medical College, Albany, New York 12208, USA.
authors.forename: A J
authors.initials: AJ
authors.lastname: Turinsky
authors.forename: T R
authors.initials: TR
authors.lastname: Moir-Blais
authors.forename: F J
authors.initials: FJ
authors.lastname: Grundy
authors.forename: T M
authors.initials: TM
authors.lastname: Henkin
mesh_terms: D000085:Acetates / Q000378:metabolism; D000093:Acetoin / Q000378:metabolism*; D000095:Acetolactate Synthase / Q000235:genetics / Q000378:metabolism*; D001412:Bacillus subtilis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D001426:Bacterial Proteins; D002262:Carboxy-Lyases / Q000235:genetics / Q000378:metabolism*; D003470:Culture Media; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D005798:Genes, Bacterial; D006863:Hydrogen-Ion Concentration; D007763:Lac Operon; D016296:Mutagenesis; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D000085:Acetates; D001426:Bacterial Proteins; D003470:Culture Media; D004268:DNA-Binding Proteins; D012097:Repressor Proteins; C069286:catabolite control proteins, bacteria; D000093:Acetoin; D000095:Acetolactate Synthase; D002262:Carboxy-Lyases; C023746:acetolactate decarboxylase