title: Production of recombinant mammalian holo-E2 and E3 and reconstitution of functional branched-chain alpha-keto acid dehydrogenase complex with recombinant E1.
authors.affiliation: Department of Biochemistry, University of Texas, Southwestern Medical Center, Dallas 75390-9038, USA.
authors.forename: J L
authors.initials: JL
authors.lastname: Chuang
authors.forename: J R
authors.initials: JR
authors.lastname: Davie
authors.forename: R M
authors.initials: RM
authors.lastname: Wynn
authors.forename: D T
authors.initials: DT
authors.lastname: Chuang
mesh_terms: D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide); D000818:Animals; D002417:Cattle; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D007658:Ketone Oxidoreductases / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D009097:Multienzyme Complexes / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism
chemical_list: D009097:Multienzyme Complexes; D011994:Recombinant Proteins; D007658:Ketone Oxidoreductases; D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)