Intratracheal administration of microcystin-LR, and its distribution.
Microcystin-LR (MCLR) was injected into mice intratracheally, absorption from the lungs was easy and it was confirmed that both the cause of death and lethality dose level were the same as by intraperitoneal injection treatment. An immunostaining method revealed that there was a time lag of about 60 min before accumulation of MCLR, and that it caused bleeding in the liver. Clearance from internal organs took about 2 weeks; during the initial stage (the first 2 days), the small intestine, kidney, cecum and large intestine were already involved. However, even after 2 weeks, small amounts of MCLR were still present in epithelial cells in the gastrointestinal mucosa.
Ito
E
E
Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Inohana, Japan.
Kondo
F
F
Harada
K
K
D000042:Absorption; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000633:toxicity*; D007274:Injections, Intraperitoneal; D007442:Intubation, Intratracheal; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008168:Lung / Q000187:drug effects / Q000473:pathology; D008387:Marine Toxins; D051379:Mice; D008813:Mice, Inbred ICR; D052998:Microcystins; D010456:Peptides, Cyclic / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000633:toxicity*; D014018:Tissue Distribution
D004791:Enzyme Inhibitors; D008387:Marine Toxins; D052998:Microcystins; D010456:Peptides, Cyclic; C057862:cyanoginosin LR