Use of cultured human fibroblasts for rapid evaluation of cytotoxic effects of bioactive substances.
A test system for detecting cytotoxic effects of bioactive substances based on human fibroblast culture is proposed. The effects of acrylamide, streptomycin, cycloheximide, sodium dodecyl sulfate, sanguiritrine, and ethanol were evaluated by organic stain binding. Typical dose-effect relationships were detected for all substances except cycloheximide. The proposed test system can be used for screening of bioactive substances in preclinical trials.
Chernikov
V G
VG
Medical Genetics Research Center, Russian Academy of Medical Sciences, Moscow.
Shishkin
S S
SS
Krokhina
T B
TB
Rebrov
L B
LB
Denisov-Nikol'skii
Y I
YI
Bykov
V A
VA
D018929:Cell Culture Techniques / Q000379:methods; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D006801:Humans