title: [Regulation of purine biosynthetic genes expression in Salmonella typhimurium].
abstract: To study binding funtion of 4 consensus bases in 16 bp PUR box with purR protein, the directed site mutation for each was carried out, which mutate from C to G, A to G, A to G, T to C, respectively. Gel retardation showed that the PUR box carrying a reserved mutation could not bind with purR protein. It suggested that all these consensus base pairs are necessary to hold the normal binding function of PUR box with purR protein.
authors.affiliation: Institutl of Microbiology, Chinese Academy of Sciences, Beijing, China.
authors.forename: H X
authors.initials: HX
authors.lastname: Long
authors.forename: W J
authors.initials: WJ
authors.lastname: MA
authors.forename: J C
authors.initials: JC
authors.lastname: Qin
authors.forename: A Q
authors.initials: AQ
authors.lastname: Wang
mesh_terms: D015964:Gene Expression Regulation, Bacterial; D016297:Mutagenesis, Site-Directed; D011687:Purines / Q000096:biosynthesis*; D012486:Salmonella typhimurium / Q000235:genetics*
chemical_list: D011687:Purines; C030985:purine