title: Anxiolytic-like effects of 4-phenyl-2-trichloromethyl-3H-1, 5-benzodiazepine hydrogen sulfate in mice.
abstract: The pharmacological effects of 4-phenyl-2-trichloromethyl-3H-1, 5-benzodiazepine hydrogen sulfate (PTMB), a novel synthetic benzodiazepine, were examined in mice. In the elevated plus-maze test of anxiety, 0.3-1 mg/kg diazepam ip (F(3,53) = 3.78; P<0.05) and 1-10 mg/kg PTMB ip increased (F(5,98) = 3.26; P<0.01), whereas 2 mg/kg picrotoxin ip decreased (F(3,59) = 8.32; P<0.001) the proportion of time spent in the open arms, consistent with an anxiolytic action of both benzodiazepines, and an anxiogenic role for picrotoxin. In the holeboard, 1.0 mg/kg diazepam ip increased (F(3,54) = 2.78; P<0.05) and 2 mg/kg picrotoxin ip decreased (F(3, 59) = 4.69; P<0.01) locomotor activity. Rotarod assessment revealed that 1 mg/kg diazepam ip and 3, 10 and 30 mg/kg PTMB ip produced significant motor incoordination compared to vehicle control (F(4, 70) = 7.6; P<0.001). These data suggest that the recently synthesized PTMB compound possesses anxiolytic activity and produces motor incoordination similar to those observed with diazepam.
authors.affiliation: Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil. marubin@quimica.ufsm.br
authors.forename: M A
authors.initials: MA
authors.lastname: Rubin
authors.forename: C A
authors.initials: CA
authors.lastname: Albach
authors.forename: D B
authors.initials: DB
authors.lastname: Berlese
authors.forename: H G
authors.initials: HG
authors.lastname: Bonacorso
authors.forename: S R
authors.initials: SR
authors.lastname: Bittencourt
authors.forename: C M
authors.initials: CM
authors.lastname: Queiroz
authors.forename: A E
authors.initials: AE
authors.lastname: Maixner
authors.forename: C F
authors.initials: CF
authors.lastname: Mello
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D014151:Anti-Anxiety Agents / Q000494:pharmacology*; D001522:Behavior, Animal / Q000187:drug effects; D001569:Benzodiazepines / Q000494:pharmacology*; D003292:Convulsants / Q000494:pharmacology; D003975:Diazepam / Q000494:pharmacology*; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D051379:Mice; D009043:Motor Activity / Q000187:drug effects*; D010852:Picrotoxin / Q000494:pharmacology
chemical_list: D014151:Anti-Anxiety Agents; D001569:Benzodiazepines; D003292:Convulsants; D003975:Diazepam; D010852:Picrotoxin; C415126:4-phenyl-2-trichloromethyl-3H-1,5-benzodiazepine