title: [Effects of gene copy number and chromosomal position on the expression of a modified HBsAg gene SA-28 in yeast].
abstract: The effect of gene copy number and chromosomal position on heterologous gene expression in Saccharomyces cerevisae has been tentatively investigated using a modified hepatitis B virus surface antigen SA-28 gene. The plasmids containing different copies of SA-28 gene expression cassette were integrated into different chromosomal loci via FLP recombinase mediated targeted integration. The expression of SA-28 gene in the resultant transformants was then measured. The result indicated that the expression of SA-28 gene was positively related to the gene dosage at HIS3 locus and had chromosomal polarity at several chromosomal loci.
authors.affiliation: State Key Laboratory of Genetic Engineering, Fudan University, Shanghai.
authors.forename: H
authors.initials: H
authors.lastname: Pan
authors.forename: X D
authors.initials: XD
authors.lastname: Gao
authors.forename: F
authors.initials: F
authors.lastname: Wang
authors.forename: H Y
authors.initials: HY
authors.lastname: Yuan
authors.forename: Y Y
authors.initials: YY
authors.lastname: Li
mesh_terms: D002874:Chromosome Mapping; D018628:Gene Dosage; D015870:Gene Expression; D006514:Hepatitis B Surface Antigens / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D006514:Hepatitis B Surface Antigens