[Integration of the genetic map and the physical map of the subterminal region on the longer arm of rice chromosome 6].
The region between RFLP markers G342 and R1167 was the subterminal part of the longer arm on the rice chromosome 6, because Shen et al. (1998) mapped two telomeric repeat associated sequences distal to G342. In order to integrate the genetic map and the physical map of the region, G342 and R1167 were firstly used to screen BAC library. Based on the positive clones detected by the two markers and chromosome walking by using the outer most insert-end of the overlapping clones, a contig containing 16 BAC clones which spanned 500 kb was constructed. All the insert-ends of the BAC clones could be amplified with thermal asymmetric interlaced PCR. Fourteen insert-ends were subcloned. Seven of them were identified as a single or low copy sequences and five were mapped on the expected sites flanking G342 or R1167. The insert fragment isolated from the minimum tile BAC clones of the contig was used to screen a cDNA library and four different positive clones were detected.
Wang
W M
WM
Institute of Genetics, Chinese Academy of Sciences, Beijing, China.
Zhai
W X
WX
Chen
C X
CX
Zheng
X W
XW
Yan
C J
CJ
Li
X B
XB
Zhu
L H
LH
D002874:Chromosome Mapping; D012275:Oryza / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length