Identification of morphine in the adrenal medullary chromaffin PC-12 cell line.
Morphine was identified in the adrenal medulla chromaffin PC-12 cell line by reversed-phase HPLC, following liquid and solid extraction. The morphine corresponding HPLC fractions (1.746+/-0.615 ng of morphine/million cells) were further analyzed by gas chromatography-mass spectrometry and found to be identical to synthetic morphine. Furthermore, using primers derived from the human neuronal mu 1 opiate receptor, we used RT-PCR to detect expression of mu transcripts from this cell line. The transcript was absent. The study conclusively proves morphine, but not a mu opiate receptor, is constitutively expressed in the adrenal medulla chromaffin PC-12 cell line.
Goumon
Y
Y
Neuroscience Research Institute, State University of New York, College at Old Westbury, Old Westbury, NY 11568, USA.
Zhu
W
W
Weeks
B S
BS
Casares
F
F
Cadet
P
P
Bougaeva
M
M
Brownawell
B
B
Stefano
G B
GB
D000310:Adrenal Gland Neoplasms / Q000737:chemistry* / Q000235:genetics; D000313:Adrenal Medulla / Q000737:chemistry; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D009020:Morphine / Q000032:analysis*; D016716:PC12 Cells; D010673:Pheochromocytoma / Q000737:chemistry* / Q000235:genetics; D051381:Rats; D017450:Receptors, Opioid, mu / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
D017450:Receptors, Opioid, mu; D009020:Morphine