title: Determination of some antirheumatics by capillary isotachophoresis.
abstract: Isotachophoresis (ITP) was applied for the determination of some antirheumatic drugs (fenoprofen, naproxen, ibuprofen, and ketoprofen) in human serum. The leading electrolyte contained hydrochloric acid (10 mmol x L(-1)), creatinine (pH 4.5) and methylhydroxyethyl cellulose (0.1%). The terminating electrolyte was 2-(N-morpholino)ethanesulfonic acid (10 mmol x L(-1)) adjusted with tris(hydroxymethyl)aminomethane to pH 6.9. The ITP separations were carried out in column-coupling configuration of the separation unit provided with a preseparation column of 160 x 0.8 mm inner diameter (ID) and analytical column of 160 x 0.3 mm ID. The limit of detection for ibuprofen, fenoprofen, and naproxen in serum by direct sampling was 0.008, 0.005 and 0.004 mmol x L(-1). The limit of detection for ketoprofen in serum after ethanol precipitation was 0.001 mmol x L(-1).
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemical Technology, Bratislava, Slovak Republic. herceg@chelin.chtf.stuba.sk
authors.forename: A
authors.initials: A
authors.lastname: Hercegová
authors.forename: J
authors.initials: J
authors.lastname: Sádecká
authors.forename: J
authors.initials: J
authors.lastname: Polonský
mesh_terms: D018501:Antirheumatic Agents / Q000097:blood*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005279:Fenoprofen / Q000097:blood; D006801:Humans; D007052:Ibuprofen / Q000097:blood; D007660:Ketoprofen / Q000097:blood; D009288:Naproxen / Q000097:blood
chemical_list: D018501:Antirheumatic Agents; D009288:Naproxen; D007660:Ketoprofen; D005279:Fenoprofen; D007052:Ibuprofen