title: Characterization of the AfaD-like family of invasins encoded by pathogenic Escherichia coli associated with intestinal and extra-intestinal infections.
abstract: The afimbrial adhesive sheath, encoded by the afa-3 gene cluster, is composed of two proteins with different roles in bacterium-HeLa cell interactions. AfaE is required for adhesion and AfaD for internalization. In this study, we found that the AfaD invasin was structurally and functionally conserved among human afa-expressing strains, independently of AfaE subtype and clinical origin of the Escherichia coli isolate. The AggB protein from enteroaggregative E. coli was also found to be an AfaD-related invasin. These data suggest that AfaD is the prototype of a family of invasins encoded by adhesion-associated operons in pathogenic E. coli.
authors.affiliation: Unité de Pathogénie Bactérienne des Muqueuses, Institut Pasteur, Paris, France.
authors.forename: M I
authors.initials: MI
authors.lastname: Garcia
authors.forename: M
authors.initials: M
authors.lastname: Jouve
authors.forename: J P
authors.initials: JP
authors.lastname: Nataro
authors.forename: P
authors.initials: P
authors.lastname: Gounon
authors.forename: C
authors.initials: C
authors.lastname: Le Bouguénec
mesh_terms: D018829:Adhesins, Bacterial; D018830:Adhesins, Escherichia coli / Q000737:chemistry* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D016466:CHO Cells; D002448:Cell Adhesion; D017124:Conserved Sequence; D006224:Cricetinae; D017931:DNA Primers / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology*; D005816:Genetic Complementation Test; D006367:HeLa Cells; D006801:Humans; D007410:Intestinal Diseases / Q000382:microbiology*; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D005810:Multigene Family; D016296:Mutagenesis; D010957:Plasmids / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014552:Urinary Tract Infections / Q000382:microbiology
chemical_list: D018829:Adhesins, Bacterial; D018830:Adhesins, Escherichia coli; C108510:AfaD protein, E coli; C108509:AfaE protein, E coli; D001426:Bacterial Proteins; D017931:DNA Primers; D011994:Recombinant Proteins; C053476:invasin, Yersinia