Direct methods and protein crystallography at low resolution.
The tools of modern direct methods are examined and their limitations for solving protein structures discussed. Direct methods need atomic resolution data (1.1-1.2 A) for structures of around 1000 atoms if no heavy atom is present. For low-resolution data, alternative approaches are necessary and these include maximum entropy, symbolic addition, Sayre's equation, group scattering factors and electron microscopy.
Gilmore
C J
CJ
Department of Chemistry, University of Glasgow, Glasgow G12 8QQ, Scotland. chris@chem.gla.ac.uk
D001426:Bacterial Proteins / Q000737:chemistry; D018360:Crystallography, X-Ray / Q000379:methods*; D008962:Models, Theoretical; D018272:Porins / Q000737:chemistry; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*
D001426:Bacterial Proteins; C109237:OmpF protein; D018272:Porins; D011506:Proteins