Responses of Entamoeba invadens to heat shock and encystation are related.
An Entamoeba invadens gene encoding a homologue of BiP/GRP78, a 70-kDa heat shock protein or chaperonin was cloned. The predicted E. invadens BiP contained an ATP-binding site, a substrate-recognition domain, and a carboxy-terminal KDEL-peptide. Messenger RNAs of E. invadens for BiP, for a 70-kDa heat shock cognate, for a cyst wall glycoprotein (Jacob), and for chitinase were all induced by heat shock and by encystation medium. The presence of Jacob in heat-shocked amebae was confirmed by confocal microscopy and suggests that heat shock and encystation responses in E. invadens are related.
Field
J
J
Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts 02115, USA.
Van Dellen
K
K
Ghosh
S K
SK
Samuelson
J
J
D000220:Adaptation, Biological; D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics*; D000091342:Endoplasmic Reticulum Chaperone BiP; D004747:Entamoeba / Q000235:genetics*; D017125:Genes, Protozoan; D006360:Heat-Shock Proteins; D018869:Heat-Shock Response / Q000235:genetics*; D018832:Molecular Chaperones / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000302:isolation & purification; D016053:RNA, Protozoan / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid
D002352:Carrier Proteins; D000091342:Endoplasmic Reticulum Chaperone BiP; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D012333:RNA, Messenger; D016053:RNA, Protozoan