[In vitro study of the interactions between bovine herpesvirus 4 and the bovine host cells].
This work was devoted to the study of the interactions between bovine herpesvirus 4 (BHV-4) and bovine cells in vitro. It led to the discovery of two interesting properties of BVH-4 replication cycle: first, the cellular receptor heparan sulfate was proven to mediate BVH-4 binding to target cells. This is the first description of the implication of heparan sulfate in the binding process of a gammaherpesvirus. Second, using synchronised cells, the replication of BVH-4 DNA was proven to be dependent on the S phase of the cell cycle. This dependence could explain some properties of BVH-4 infection in vitro and could play an important role in the biology of the infection in vivo. Finally, in order to produce monoclonal antibodies against BVH-4 IE1 and IE2 proteins, the genes coding for these proteins were cloned and expressed in prokaryotic cells.
Vanderplasschen
A
A
D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics / Q000502:physiology; D018142:Gammaherpesvirinae / Q000235:genetics / Q000254:growth & development* / Q000276:immunology; D006497:Heparan Sulfate / Q000502:physiology; D006566:Herpesviridae Infections / Q000662:veterinary* / Q000821:virology*; D016196:S Phase / Q000502:physiology; D014776:Virus Cultivation; D016662:Virus Integration / Q000502:physiology*; D014779:Virus Replication / Q000502:physiology*
D004279:DNA, Viral; D006497:Heparan Sulfate