Ehrlich ascites tumour unbalances splenic cell populations and reduces responsiveness of T cells to Staphylococcus aureus enterotoxin B stimulation.
Tumours must avoid host immune response to survive and proliferate; to achieve this purpose, tumours interact with cells of the immune system by means of tumour secreted factors. The alterations of splenic cell populations in mice bearing the Ehrlich ascites tumour have been studied. A rapid and acute response was observed, characterized by a decrease in both CD4 and CD8 T cells, and a transient increase in the number of B cells, which peaked 2 days after tumour inoculation. An increase in macrophage population and in the homing antigen CD18 was also detected. In vitro incubations of splenic cells with the Staphylococcus aureus enterotoxin B (SEB) showed that tumour induces a state of reduced responsiveness to stimulation of T cells, mainly affecting CD8 T cells, and a diminished IFN-gamma expression.
Segura
J A
JA
Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, 29071, Málaga, Spain. jsegura@uma.es
Barbero
L G
LG
Márquez
J
J
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D018821:CD18 Antigens / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D002286:Carcinoma, Ehrlich Tumor / Q000276:immunology*; D018929:Cell Culture Techniques; D004768:Enterotoxins / Q000494:pharmacology*; D005434:Flow Cytometry; D007371:Interferon-gamma / Q000276:immunology; D008213:Lymphocyte Activation; D018655:Lymphocyte Count; D008264:Macrophages / Q000276:immunology; D051379:Mice; D009368:Neoplasm Transplantation; D013154:Spleen / Q000166:cytology* / Q000276:immunology*
D018821:CD18 Antigens; D004768:Enterotoxins; C031818:enterotoxin B, staphylococcal; D007371:Interferon-gamma