title: Levels of house-dust-mite allergen in homes of nonallergic people in Pavia, Italy.
abstract: BACKGROUND
  HDM distribution varies between geographic areas and may be affected by housing characteristics. We quantified Der p 1 and Der f 1 and assessed the relationships between their levels and housing characteristics in homes of nonallergic subjects.
  
  
  METHODS
  Der f 1 and Der p 1 were measured by ELISA in dust samples from living-room floors and mattresses of 44 homes of nonallergic subjects in Pavia. Information about household characteristics was obtained by questionnaire.
  
  
  RESULTS
  Der p 1 and Der f 1 concentrations (microg/g dust, median) were 0.34 and 7.8 on mattresses, and 0.15 and 0.83 on living-room floors. Higher Der f 1 levels on mattresses were associated with synthetic pillows (P<0.05), and (only when expressed as microg/m2) with bedding washing temperature of < or = 60 degrees C (P<0.05). Der f 1 levels were higher on the living-room floors of homes located on lower (< or =first) floors (P<0.05). Good correlations were found between Der p 1 and Der f 1 expressed as microg/g dust and microg/m2 on both mattresses and living-room floors.
  
  
  CONCLUSIONS
  In homes of nonallergic subjects in northern Italy, Der f 1 exceeded the threshold for sensitization in a high proportion of mattress samples.
authors.affiliation: Fondazione S. Maugeri, Clinica del Lavoro e della Riabilitazione, IRCCS, Istituto Scientifico di Pavia, Allergy and Clinical Immunology Unit, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Moscato
authors.forename: L
authors.initials: L
authors.lastname: Perfetti
authors.forename: E
authors.initials: E
authors.lastname: Galdi
authors.forename: V
authors.initials: V
authors.lastname: Pozzi
authors.forename: C
authors.initials: C
authors.lastname: Minoia
mesh_terms: D000818:Animals; D039741:Antigens, Dermatophagoides; D001513:Beds; D004391:Dust / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005430:Floors and Floorcoverings; D006023:Glycoproteins / Q000032:analysis*; D006798:Housing; D006801:Humans; D007558:Italy; D008925:Mites / Q000276:immunology*
chemical_list: D039741:Antigens, Dermatophagoides; D004391:Dust; D006023:Glycoproteins