[Effects of gene copy number and chromosomal position on the expression of a modified HBsAg gene SA-28 in yeast].
The effect of gene copy number and chromosomal position on heterologous gene expression in Saccharomyces cerevisae has been tentatively investigated using a modified hepatitis B virus surface antigen SA-28 gene. The plasmids containing different copies of SA-28 gene expression cassette were integrated into different chromosomal loci via FLP recombinase mediated targeted integration. The expression of SA-28 gene in the resultant transformants was then measured. The result indicated that the expression of SA-28 gene was positively related to the gene dosage at HIS3 locus and had chromosomal polarity at several chromosomal loci.
Pan
H
H
State Key Laboratory of Genetic Engineering, Fudan University, Shanghai.
Gao
X D
XD
Wang
F
F
Yuan
H Y
HY
Li
Y Y
YY
D002874:Chromosome Mapping; D018628:Gene Dosage; D015870:Gene Expression; D006514:Hepatitis B Surface Antigens / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D006514:Hepatitis B Surface Antigens