Proteomics of Synechocystis sp. strain PCC 6803. Identification of periplasmic proteins in cells grown at low and high salt concentrations.
Periplasmic proteins isolated by cold osmotic shock of Synechocystis sp. PCC 6803 cells were identified using 2D PAGE, MS and genome analysis. Most of the periplasmic proteins represent 'hypothetical proteins' with unknown function. A number of proteases of different specificity, and several enzymes involved in cell wall biosynthesis were also found. In salt-adapted cells, six proteins were greatly enhanced and three proteins were newly induced. Most of the salt-enhanced proteins are involved in the alteration of cell wall structure of salt-adapted cells. The precursors of all 57 periplasmic proteins identified have a signal peptide; 47 of them contain a typical Sec-dependent signal peptide, whereas 10 contain a putative twin-arginine signal peptide.
Fulda
S
S
Institut für Molekulare Physiologie und Biotechnologie, Universität Rostock, Germany. sf@bio4.uni-rostock.de
Huang
F
F
Nilsson
F
F
Hagemann
M
M
Norling
B
B
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D002473:Cell Wall / Q000378:metabolism; D003470:Culture Media / Q000494:pharmacology*; D000458:Cyanobacteria / Q000737:chemistry* / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D007038:Hypotonic Solutions / Q000494:pharmacology*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009994:Osmolar Concentration; D009997:Osmotic Pressure; D019897:Periplasm / Q000737:chemistry*; D011498:Protein Precursors / Q000378:metabolism; D021382:Protein Sorting Signals; D020543:Proteome; D012462:Saline Solution, Hypertonic / Q000494:pharmacology*; D017386:Sequence Homology, Amino Acid; D012965:Sodium Chloride / Q000494:pharmacology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001426:Bacterial Proteins; D003470:Culture Media; D007038:Hypotonic Solutions; D011498:Protein Precursors; D021382:Protein Sorting Signals; D020543:Proteome; D012462:Saline Solution, Hypertonic; D012965:Sodium Chloride