Structure of succinoglycan from an infectious strain of Agrobacterium radiobacter.
The exopolysaccharide produced by a cystic fibrosis clinical isolate of Agrobacterium radiobacter was shown by monosaccharide and methylation analyses, degradation with succinoglycanase and NMR analysis to be a succinoglycan with the structure shown below. (S)-pyruvic acid is found stoichiometrically as 4,6-O-ketal substituent of terminal glucose. Succinic acid is present in 40% of the repeating units and it is attached to O-6 of the 3-linked glucose next to the pyruvate carrying sugar. Some evidence is found that a small amount of succinic acid (ca. 6% of the total) is linked to O-6 of another undetermined glucose. [structure: see text]
Evans
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Department of Veteran Affairs Medical Center, 500 Foothill Boulevard (151), 84148, Salt Lake City, UT, USA.
Linker
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Impallomeni
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D002240:Carbohydrate Sequence; D002250:Carbon Radioisotopes; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D019289:Pyruvic Acid / Q000378:metabolism; D012231:Rhizobium / Q000737:chemistry*; D013386:Succinates / Q000378:metabolism
D002250:Carbon Radioisotopes; D011135:Polysaccharides, Bacterial; D013386:Succinates; C056779:succinoglycan; D019289:Pyruvic Acid