title: Self-cloning minitransposon phoA gene-fusion system promotes the rapid genetic analysis of secreted proteins in gram-negative bacteria.
authors.affiliation: University of Calgary Health Sciences Centre, AB, Canada.
authors.forename: A J
authors.initials: AJ
authors.lastname: Bolton
authors.forename: D E
authors.initials: DE
authors.lastname: Woods
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D018844:Cyclin-Dependent Kinases / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D006090:Gram-Negative Bacteria / Q000235:genetics*; D016296:Mutagenesis; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D011993:Recombinant Fusion Proteins; D018844:Cyclin-Dependent Kinases; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; C029312:GGTACC-specific type II deoxyribonucleases; D000469:Alkaline Phosphatase; C114089:phoA protein, E coli