[Determination of the concentration of ligand-specific molecules, found in mixtures with ligand-nonspecific molecules].
A new method, which allows determination of the concentration of ligand-specific molecules in a mixture of these molecules with biochemically similar but ligand-unspecific molecules, is suggested. The method is based on a partial exhaustion of the mixture on a column with immobilized ligand and determination of the part of ligand-specific molecules presented in exhausted mixture. The concentration of monoclonal antibodies specific to bovine serum albumin in a commercial "Sigma" preparation and concentration of polyreactive immunoglobulins in a commercial "Sigma" preparation of bovine immunoglobulins were determined by suggested method.
Bobrovnik
S A
SA
A. V. Palladin Institute of Biochemistry, Kyiv, Ukraine. sab@biochem.kiev.ua
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002417:Cattle; D008024:Ligands; D008722:Methods; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000276:immunology*
D000911:Antibodies, Monoclonal; D008024:Ligands; D012710:Serum Albumin, Bovine