Pseudomonas aeruginosa ExoT is a Rho GTPase-activating protein.
Transient intracellular expression of ExoT in CHO cells stimulated cell rounding and actin reorganization. Biochemical studies showed that ExoT was a GTPase-activating protein for RhoA, Rac1, and Cdc42. Together, these data show that ExoT interferes with Rho signal transduction pathways, which regulate actin organization, exocytosis, cell cycle progression, and phagocytosis.
Krall
R
R
Medical College of Wisconsin, Microbiology and Molecular Genetics, Milwaukee, Wisconsin 53226, USA.
Schmidt
G
G
Aktories
K
K
Barbieri
J T
JT
D000199:Actins / Q000378:metabolism; D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003603:Cytotoxins / Q000235:genetics* / Q000378:metabolism*; D020690:GTPase-Activating Proteins / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000472:pathogenicity*; D014162:Transfection
D000199:Actins; D003603:Cytotoxins; D020690:GTPase-Activating Proteins; C085379:rho GTPase-activating protein