Characterisation of isolated alpha-synuclein filaments from substantia nigra of Parkinson's disease brain.
The defining neuropathological deposits of Parkinson's disease, dementia with Lewy bodies and multiple system atrophy are strongly immunoreactive for alpha-synuclein. We have shown previously that isolated filaments from dementia with Lewy bodies and multiple system atrophy brains are labelled in a characteristic fashion by a number of alpha-synuclein antibodies. Here we have extracted filaments from substantia nigra of patients with idiopathic Parkinson's disease. Antibodies directed against the carboxy-terminal region of alpha-synuclein labelled isolated filaments along their entire lengths. By contrast, an antibody directed against the amino-terminal region of alpha-synuclein only labelled one filament end. These characteristics were identical to those of filaments extracted from brains of patients with dementia with Lewy bodies and multiple system atrophy.
Crowther
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RA
Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK.
Daniel
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SE
Goedert
M
M
D000368:Aged; D000369:Aged, 80 and over; D000906:Antibodies; D003599:Cytoskeleton / Q000737:chemistry / Q000473:pathology / Q000648:ultrastructure; D006801:Humans; D020961:Lewy Body Disease / Q000473:pathology; D008854:Microscopy, Electron; D019578:Multiple System Atrophy / Q000473:pathology; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000276:immunology; D010300:Parkinson Disease / Q000473:pathology*; D013378:Substantia Nigra / Q000737:chemistry* / Q000473:pathology*; D051843:Synucleins; D051844:alpha-Synuclein
D000906:Antibodies; D009419:Nerve Tissue Proteins; C497604:SNCA protein, human; D051843:Synucleins; D051844:alpha-Synuclein