title: Purification and characterization of two hemolysins from Stichodactyla helianthus.
abstract: Two hemolysins, Sticholysin I (St I) and Sticholysin II (St II) were purified from the sea anemone Stichodactyla helianthus combining gel filtration and ion exchange chromatography. The amino acid composition of both cytolysins was determined revealing a high proportion of glycine, lysine, tyrosine and non-polar amino acids (alanine, leucine and valine). Cysteine was not found in either polypeptide. Molecular masses of St I and St II were 19401 and 19290 Da, respectively. N-terminal sequence analysis of St I and St II showed a high homology between them suggesting they are isoforms of the same cytolysin. Compared with other sea anemone cytolysins, St I and St II contain a 22 amino acid insertion fragment also present in Eq T II/Tn C and probably in CaT I and Hm T and absent in C III, the major hemolysin previously reported in this anemone.
authors.affiliation: Department of Biochemistry, University of Havana, Cuba.
authors.forename: M E
authors.initials: ME
authors.lastname: Lanio
authors.forename: V
authors.initials: V
authors.lastname: Morera
authors.forename: C
authors.initials: C
authors.lastname: Alvarez
authors.forename: M
authors.initials: M
authors.lastname: Tejuca
authors.forename: T
authors.initials: T
authors.lastname: Gómez
authors.forename: F
authors.initials: F
authors.lastname: Pazos
authors.forename: V
authors.initials: V
authors.lastname: Besada
authors.forename: D
authors.initials: D
authors.lastname: Martínez
authors.forename: V
authors.initials: V
authors.lastname: Huerta
authors.forename: G
authors.initials: G
authors.lastname: Padrón
authors.forename: M
authors.initials: M
authors.lastname: de los Angeles Chávez
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003064:Cnidarian Venoms / Q000032:analysis / Q000302:isolation & purification*; D006460:Hemolysin Proteins / Q000032:analysis / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009930:Organic Chemicals; D010455:Peptides / Q000032:analysis / Q000302:isolation & purification*; D012615:Sea Anemones
chemical_list: D003064:Cnidarian Venoms; D006460:Hemolysin Proteins; D009930:Organic Chemicals; D010455:Peptides; C404794:sticholysin II; C121348:stycholysin I