Isolation and characterization of Myrianthus holstii lectin, a potent HIV-1 inhibitory protein from the plant Myrianthus holstii(1).
Aqueous extracts from the African plant Myrianthus holstii potently inhibited the infection of the T-lymphoblastoid cell line, CEM-SS, by human immunodeficiency virus-1(RF) (HIV-1(RF)). The active constituent, M. holstii lectin (MHL), was purified by LH-20 column chromatography and reversed phase HPLC. MHL, a 9284-Da cysteine-rich protein, was characterized by amino acid analysis, N-terminal sequencing, ESIMS, and matrix-assisted laser-desorption ionization-time-of-flight mass spectrometry. Pure MHL had anti-HIV activity, with an EC(50) value of 150 nM. Delaying the addition of MHL for up to 8 h after initial exposure of CEM-SS cells to virus did not result in loss of the antiviral activity; however, if addition of the compound was delayed for 16 h or more, there was a marked decrease in the antiviral activity. MHL bound to a virus-free, soluble form of the viral envelope protein gp120 but did not inhibit the subsequent binding to a cell-free, soluble form of the cellular receptor CD4.
Charan
R D
RD
Division of Cancer Treatment and Diagnosis, National Cancer Institute, Frederick Cancer Research and Development Center, Building 1052, Room 121, Frederick, MD 21702-1201, USA.
Munro
M H
MH
O'Keefe
B R
BR
Sowder RCII
McKee
T C
TC
Currens
M J
MJ
Pannell
L K
LK
Boyd
M R
MR
D000595:Amino Acid Sequence; D015704:CD4 Antigens / Q000737:chemistry; D002449:Cell Aggregation / Q000187:drug effects; D002478:Cells, Cultured; D002688:Chitinases / Q000032:analysis; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D008401:Gas Chromatography-Mass Spectrometry; D015699:HIV Envelope Protein gp120 / Q000737:chemistry; D015658:HIV Infections / Q000188:drug therapy; D015497:HIV-1 / Q000187:drug effects*; D006386:Hemagglutination Tests; D006801:Humans; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D008969:Molecular Sequence Data; D037121:Plant Lectins; D018517:Plant Roots / Q000737:chemistry*; D010944:Plants / Q000737:chemistry*; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013636:Tanzania
D015704:CD4 Antigens; D015699:HIV Envelope Protein gp120; D037102:Lectins; D037121:Plant Lectins; C414474:lectin, Myrianthus holstii; D002688:Chitinases