title: Antioxidant binding of caeruloplasmin to myeloperoxidase: myeloperoxidase is inhibited, but oxidase, peroxidase and immunoreactive properties of caeruloplasmin remain intact.
abstract: The neutrophil enzyme myeloperoxidase (MPO) purposefully makes hypochlorous acid (HOCl) as part of the cells defence against microbial infections. During cell lysis, however, MPO will be released into the extracellular environment where production of HOCl, a powerful oxidant, will lead to molecular damage. Extracellular MPO binds to the copper-containing protein caeruloplasmin (Cp) and prevents MPO making HOCl. Cp has several important antioxidant functions in extracellular fluids associated with its ability to catalyse oxidation of ferrous ions and to remove peroxides. The binding of MPO to Cp did not inhibit these important extracellular antioxidant activities of Cp, but in so doing it provided additional antioxidant protection against formation of HOCl.
authors.affiliation: Department of Biochemistry, Osaka University Medical School, Suita, Japan.
authors.forename: Y S
authors.initials: YS
authors.lastname: Park
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: S
authors.initials: S
authors.lastname: Mumby
authors.forename: N
authors.initials: N
authors.lastname: Taniguchi
authors.forename: J M
authors.initials: JM
authors.lastname: Gutteridge
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D002570:Ceruloplasmin / Q000276:immunology / Q000378:metabolism*; D005296:Ferrous Compounds / Q000378:metabolism; D006801:Humans; D006997:Hypochlorous Acid / Q000378:metabolism; D007700:Kinetics; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism; D009195:Peroxidase / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D005296:Ferrous Compounds; D006997:Hypochlorous Acid; D010088:Oxidoreductases; D009195:Peroxidase; D002570:Ceruloplasmin