title: In vivo effects of Hb solutions on blood viscosity and rheologic behavior of RBCs: comparison with clinically used volume expanders.
abstract: BACKGROUND
  Hb-based oxygen carriers (HbOCs) have vasoactive effects that are still poorly understood. Factors known to have vasoactive effects, such as plasma, whole-blood viscosity, and the rheologic behavior of RBCs, are modulated by HbOCs in vitro, but few in vivo studies have been performed.
  
  
  METHODS
  Rabbits were phlebotomized (30%) and resuscitated with unmodified stroma-free Hb (SFHb), dextran-tetracarboxylate-Hb (Dex-BTC-Hb), O-raffinose-polymerized Hb (OrpHb), HSA, or hydroxyethyl starch 200 (HES). Plasma viscosity was assessed with a capillary viscometer and whole-blood viscosity with a rotational viscosimeter. RBC aggregation kinetics were determined by analysis of back-scattered light in a rotating device.
  
  
  RESULTS
  As compared to that in the control RBC suspension, resuscitation with SFHb, OrpHb, or HSA decreased plasma and whole-blood viscosity as well as RBC aggregation; resuscitation with Dex-BTC-Hb increased whole-blood viscosity at low shear rates as well as RBC aggregation, whereas that with HES decreased whole-blood viscosity but increased RBC aggregation.
  
  
  CONCLUSIONS
  HbOCs have different rheologic effects in vitro and in vivo. There are marked differences among the Hb solutions in their in vivo effects on viscosity and RBC rheologic behavior (especially at low shear rates encountered in the venous circulation and the microcirculation), which may be related to the chemical modifications applied to hemoprotein. These results could contribute to an understanding of the vasoactive effects of HbOCs.
authors.affiliation: Departments of Physiology and Angiohematology-Hemorheology, Henri Poincaré University, Nancy, France. menu@pharma.u-nancy.fr
authors.forename: P
authors.initials: P
authors.lastname: Menu
authors.forename: W
authors.initials: W
authors.lastname: Bleeker
authors.forename: D
authors.initials: D
authors.lastname: Longrois
authors.forename: A
authors.initials: A
authors.lastname: Caron
authors.forename: B
authors.initials: B
authors.lastname: Faivre-Fiorina
authors.forename: S
authors.initials: S
authors.lastname: Muller
authors.forename: P
authors.initials: P
authors.lastname: Labrude
authors.forename: J F
authors.initials: JF
authors.lastname: Stoltz
mesh_terms: D000328:Adult; D000818:Animals; D001809:Blood Viscosity / Q000187:drug effects*; D004903:Erythrocyte Aggregation / Q000502:physiology; D004912:Erythrocytes / Q000502:physiology*; D006454:Hemoglobins / Q000494:pharmacology*; D018056:Hemorheology / Q000187:drug effects; D006801:Humans; D008297:Male; D009996:Osmotic Fragility / Q000187:drug effects; D011817:Rabbits; D012151:Resuscitation; D012996:Solutions / Q000494:pharmacology
chemical_list: D006454:Hemoglobins; D012996:Solutions