A method for detecting antibody internalization in drug targeting.
BCM43/2E5 monoclonal antibody with a specificity for human ovarian carcinoma was evaluated for its binding and internalization behaviour on both fixed and live cells. Internalization of this antibody by the targeted cells was demonstrated by ELISA, flow cytometry and immunoperoxidase. At reduced temperatures or in the presence of electron transfer blockers, internalization of bound antibody was prevented. This method is rapid and reproducible and may be used efficiently for the selection of antibodies suitable for drug targeting in cancer therapy.
Jaime
J
J
Department of Medical Biology, Faculty of Medicine, Universite Laval, STE-Foy, Quebec, Canada.
Pagé
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D016475:3T3 Cells; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000378:metabolism / Q000493:pharmacokinetics*; D000918:Antibody Specificity; D016503:Drug Delivery Systems; D004705:Endocytosis / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods; D051379:Mice; D008856:Microscopy, Fluorescence; D010051:Ovarian Neoplasms / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D011485:Protein Binding; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000276:immunology / Q000378:metabolism
D000911:Antibodies, Monoclonal