Cloning, characterization and chromosomal assignment of NBC4, a new member of the sodium bicarbonate cotransporter family.
We report the cloning, characterization and chromosomal assignment of a new member of the sodium bicarbonate cotransporter (NBC) family, NBC4, from human heart. NBC4 maps to chromosome 2p13 and is a new candidate gene for Alstrom syndrome. NBC4 encodes a 1074-residue polypeptide with 12 putative membrane-spanning domains. Unlike other members of the NBC family, NBC4 has a unique glycine-rich region (amino acids 438-485). In addition, NBC4 lacks the lysine-rich C-terminus of NBC1 with which it is most homologous. The first of two putative stilbene binding motifs (K(M/L)(X)K) is lacking in NBC4 (amino acids 655-658). The approximately 6 kb NBC4 transcript is moderately expressed in heart, with the highest expression in liver, testes and spleen.
Pushkin
A
A
Division of Nephrology, UCLA School of Medicine, 10833 Le Conte Avenue, Room 7-155 Factor Building, 90095-1689, Los Angeles, CA, USA.
Abuladze
N
N
Newman
D
D
Lee
I
I
Xu
G
G
Kurtz
I
I
D000595:Amino Acid Sequence; D001639:Bicarbonates / Q000378:metabolism; D001665:Binding Sites; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics; D006319:Hearing Loss, Sensorineural / Q000235:genetics; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008565:Membrane Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D009765:Obesity / Q000235:genetics; D017433:Protein Structure, Secondary; D016415:Sequence Alignment; D027982:Sodium-Bicarbonate Symporters; D013577:Syndrome
D001639:Bicarbonates; D002352:Carrier Proteins; D008565:Membrane Proteins; D027982:Sodium-Bicarbonate Symporters