title: Plant regeneration from single-nodal-stem explants of legume tree Prosopis alba Griseb.
abstract: Seeds of Prosopis alba were scarified with abrasive paper and placed to germinate on MS (Murashige and Skoog 1962) nutrient medium. After 7 days of culture, the basal part of cotyledons was removed and pieces of 4 mm" from distal parts were cultured on Murashige and Skoog (1962) mineral salts and vitamins (MS) (3% sucrose) supplemented with growth regulators. Callus proliferation took place in the majority of the media tested. A low percentage of calluses with green buds that developed on MS basal medium containing 0.1 mg.L-1 2,4-D alone or supplemented with BAP at 0.1 mg.L-1 was observed. Neither cotyledonary segments in any medium assayed regenerated the whole plants. Bud elongation (near 70%) was achieved when single-nodal-stem segments cut from 20 days old seedlings were cultured on MS salts supplemented with 3 mg.L-1 NAA or 3 mg.L-1 IBA combined with 0.05 mg.L-1 KIN after 60 days in culture. Multiple shoots per bud were also observed. Single-nodal-stem segments from five-year-old plants were also cultured on the same media used for seedling explants. Maximal frequency of explants with bud elongation (near 70%) was found on MS with 0.1 mg.L-1 NAA plus 1 mg.L-1 BAP after 60 days of culture. Single-nodal-stem explants cut from adult trees (more than 20 years) were also employed, but the number of bud elongation was lesser. For rooting, the elongated shoots were transferred to a semisolid or liquid MS culture medium employing a paper bridge, supplemented with 0.5 mg.L-1 IBA or 0.1 mg.L-1 NAA.
authors.affiliation: Facultad de Recursos Naturales Renovables, Universidad Nacional de Formosa, Argentina. gmeier@arnet.com.ar
authors.forename: G
authors.initials: G
authors.lastname: Castillo de Meier
authors.forename: O A
authors.initials: OA
authors.lastname: Bovo
mesh_terms: D000367:Age Factors; D002478:Cells, Cultured; D018548:Cotyledon / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D007887:Fabaceae / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D007210:Indoleacetic Acids / Q000378:metabolism / Q000494:pharmacology; D063245:Plant Development; D018547:Plant Stems / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D010944:Plants / Q000187:drug effects / Q000378:metabolism; D010946:Plants, Medicinal; D012038:Regeneration / Q000502:physiology*; D014197:Trees / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism
chemical_list: D007210:Indoleacetic Acids