Cellular calcium imaging: so, what's new?
The ability to uncover the fine details of intracellular Ca(2+) signals has improved remarkably in recent years, largely as a result of developments in methods for reporting Ca(2+), coupled with great improvements in measurement instrumentation. The cell biologist wishing to image intracellular Ca(2+) has a range of options to consider. These include the use of photoproteins, commercially available fluorescent indicators or the new generation of fluorescent protein Ca(2+) probes. Molecular biology and biophysics are now joining forces to bring major advances in the art of deciphering the complexity of spatiotemporal Ca(2+) signals.
Brownlee
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Marine Biological Association of the UK, The Laboratory, Citadel Hill, Plymouth, UK PL1 2PB. cbr@mba.ac.uk
D000818:Animals; D002118:Calcium / Q000032:analysis*; D020013:Calcium Signaling / Q000502:physiology*; D005456:Fluorescent Dyes; D019044:Image Cytometry / Q000379:methods* / Q000639:trends*; D018613:Microscopy, Confocal / Q000379:methods / Q000639:trends; D010063:Ovum / Q000737:chemistry / Q000502:physiology
D005456:Fluorescent Dyes; D002118:Calcium