The RPM1 plant disease resistance gene facilitates a rapid and sustained increase in cytosolic calcium that is necessary for the oxidative burst and hypersensitive cell death.
Early events occurring during the hypersensitive resistance response (HR) were examined using the avrRpm1/RPM1 gene-for-gene interaction in Arabidopsis challenged by Pseudomonas syringae pv. tomato. Increases in cytosolic Ca2+ were measured in whole leaves using aequorin-mediated bioluminescence. During the HR a sustained increase in Ca2+ was observed which was dependent on the presence of both a functional RPM1 gene product and delivery of the cognate avirulence gene product AvrRpm1. The sequence-unrelated avirulence gene avrB, which also interacts with RPM1, generated a significantly later but similarly prolonged increase in cytosolic Ca2+. Accumulation of H2O2 at reaction sites, as revealed by electron microscopy, occurred within the same time frame as the changes in cytosolic Ca2+. The NADPH oxidase inhibitor diphenylene iodonium chloride did not affect the calcium signature, but did block H2O2 accumulation and the HR. By contrast, the calcium-channel blocker LaCl3 suppressed the increase in cytosolic Ca2+ as well as H2O2 accumulation and the HR, placing calcium elevation upstream of the oxidative burst.
Grant
M
M
Department of Biological Sciences, Wye College, Wye, Kent TN25 5AH, UK. m.grant@wye.ac.uk
Brown
I
I
Adams
S
S
Knight
M
M
Ainslie
A
A
Mansfield
J
J
D000331:Aequorin; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D020013:Calcium Signaling; D016923:Cell Death; D003600:Cytosol / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000378:metabolism; D007811:Lanthanum / Q000494:pharmacology; D008163:Luminescent Measurements; D008854:Microscopy, Electron; D019255:NADPH Oxidases / Q000037:antagonists & inhibitors; D009861:Onium Compounds / Q000494:pharmacology; D010101:Oxygen Consumption; D010935:Plant Diseases / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011549:Pseudomonas / Q000472:pathogenicity*; D017382:Reactive Oxygen Species / Q000378:metabolism
D029681:Arabidopsis Proteins; C100931:AvrB protein, Pseudomonas syringae; D001426:Bacterial Proteins; D004791:Enzyme Inhibitors; D009861:Onium Compounds; D010940:Plant Proteins; C095014:RPM1 protein, Arabidopsis; D017382:Reactive Oxygen Species; C028521:lanthanum chloride; D000331:Aequorin; C007517:diphenyleneiodonium; D007811:Lanthanum; D006861:Hydrogen Peroxide; D019255:NADPH Oxidases