title: Loss of phospholipid asymmetry and surface exposure of phosphatidylserine is required for phagocytosis of apoptotic cells by macrophages and fibroblasts.
abstract: Removal of apoptotic cells during tissue remodeling or resolution of inflammation is critical to the restoration of normal tissue structure and function. During apoptosis, early surface changes occur, which trigger recognition and removal by macrophages and other phagocytes. Loss of phospholipid asymmetry results in exposure of phosphatidylserine (PS), one of the surface markers recognized by macrophages. However, a number of receptors have been reported to mediate macrophage recognition of apoptotic cells, not all of which bind to phosphatidylserine. We therefore examined the role of membrane phospholipid symmetrization and PS externalization in uptake of apoptotic cells by mouse macrophages and human HT-1080 fibrosarcoma cells by exposing them to cells that had undergone apoptosis without loss of phospholipid asymmetry. Neither mouse macrophages nor HT-1080 cells recognized or engulfed apoptotic targets that failed to express PS, in comparison to PS-expressing apoptotic cells. If, however, their outer leaflets were repleted with the l-, but not the d-, stereoisomer of sn-1,2-PS by liposome transfer, engulfment by both phagocytes was restored. These observations directly demonstrate that loss of phospholipid asymmetry and PS expression is required for phagocyte engulfment of apoptotic cells and imply a critical, if not obligatory, role for PS recognition in the uptake process.
authors.affiliation: Program in Cell Biology, Department of Pediatrics, National Jewish Medical and Research Center, Denver, Colorado 80206 , USA. fadokv@njc.org
authors.forename: V A
authors.initials: VA
authors.lastname: Fadok
authors.lastname: de Cathelineau A
authors.forename: D L
authors.initials: DL
authors.lastname: Daleke
authors.forename: P M
authors.initials: PM
authors.lastname: Henson
authors.forename: D L
authors.initials: DL
authors.lastname: Bratton
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D001854:Bone Marrow Cells / Q000166:cytology; D002117:Calcitriol / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D000518:Eflornithine / Q000494:pharmacology; D005347:Fibroblasts / Q000502:physiology*; D005354:Fibrosarcoma; D018922:HL-60 Cells; D006801:Humans; D019169:Jurkat Cells; D008081:Liposomes; D008264:Macrophages / Q000502:physiology*; D008563:Membrane Lipids / Q000737:chemistry / Q000502:physiology*; D051379:Mice; D008809:Mice, Inbred C3H; D010587:Phagocytosis / Q000502:physiology*; D010718:Phosphatidylserines / Q000737:chemistry* / Q000502:physiology*; D013237:Stereoisomerism; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays
chemical_list: D008081:Liposomes; D008563:Membrane Lipids; D010718:Phosphatidylserines; D002117:Calcitriol; D000518:Eflornithine