title: IgE production after antigen-specific and cognate activation of HLA-DPw4-restricted T-cell clones, by 78% of randomly selected B-cell donors.
abstract: The frequency of expression of the MHC class II antigen, HLA-DPw4, in the caucasoid population is approximately 78%, and is unmatched by phenotypic frequencies of other HLA class II molecules. Here we describe three human Der-P1-specific T-cell clones (TCC), restricted by the HLA-DPw4-variant HLA-DPB1*0401, of which two TCC also responded to antigen, presented on HLA-DPB1*0402. Thus, randomly selected caucasoid donors present a 78% chance for a correct match with these HLA-DPw4-restricted TCC. This allows comparative in vitro antigen presentation studies with various antigen presenting cells (APC) from different (healthy or diseased) donors without the variable influence of responding T cells. It was subsequently demonstrated that the TCC can be used to study antigen-induced IgE production in randomly selected primary B cells. Cognate HLA-DPw4-restricted antigen presentation caused enhanced immunoglobulin production of IgE, IgG1, IgA and IgM, of which only IgE induction was reversed by addition of anti-IL-4 antibodies.
authors.affiliation: Novartis Research Institute, Department of Immunology, Vienna, Austria. pjbaselmans@yahoo.com
authors.forename: P J
authors.initials: PJ
authors.lastname: Baselmans
authors.forename: E
authors.initials: E
authors.lastname: Pöllabauer
authors.forename: F C
authors.initials: FC
authors.lastname: van Reijsen
authors.forename: H C
authors.initials: HC
authors.lastname: Heystek
authors.forename: A
authors.initials: A
authors.lastname: Hren
authors.forename: P
authors.initials: P
authors.lastname: Stumptner
authors.forename: M G
authors.initials: MG
authors.lastname: Tilanus
authors.forename: W C
authors.initials: WC
authors.lastname: Vooijs
authors.forename: G C
authors.initials: GC
authors.lastname: Mudde
mesh_terms: D000938:Antigen-Presenting Cells / Q000276:immunology; D039741:Antigens, Dermatophagoides; D001402:B-Lymphocytes / Q000276:immunology*; D002455:Cell Division; D002461:Cell Line, Transformed; D002999:Clone Cells; D006023:Glycoproteins / Q000276:immunology; D006682:HLA-DP Antigens / Q000276:immunology*; D059868:HLA-DP beta-Chains; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis*; D014066:Palatine Tonsil / Q000166:cytology; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014019:Tissue Donors
chemical_list: D039741:Antigens, Dermatophagoides; D006023:Glycoproteins; D006682:HLA-DP Antigens; D059868:HLA-DP beta-Chains; C071170:HLA-DPB1 antigen; C056284:HLA-DPw4 antigen; D011948:Receptors, Antigen, T-Cell; D007073:Immunoglobulin E