title: Effect of vanillin on methylene blue plus light-induced single-strand breaks in plasmid pBR322 DNA.
abstract: The ability of vanillin (4-hydroxy-3-methoxybenzaldehyde), a naturally occurring food flavouring agent, in inhibiting photosensitization-induced single-strand breaks (ssbs) in plasmid pBR322 DNA has been examined in an in vitro system, independent of DNA repair/replication processes. Photosensitization of DNA with methylene blue, visible light and oxygen, induced ssbs resulting in the production of open circular form (OC form) in a concentration-dependent manner. The yield of OC form induced by photosensitization was increased several-fold by deuteration of the buffer and was found to be inhibited by sodium azide, a scavenger of singlet oxygen (1O(2)). Vanillin, per se, did not induce but inhibited photosensitization-induced ssbs in plasmid DNA, at millimolar concentrations. The inhibitory effect of vanillin was both concentration- and time-dependent. On a molar basis, vanillin was, however, less effective than trolox, a water-soluble analogue of alpha-tocopherol. Photosensitization by methylene blue system generates singlet oxygen, as one of the major components of ROS. Therefore, interaction of singlet oxygen with vanillin was investigated. The rate constant of vanillin with 1O(2) was estimated to be 5.93x10(7)M(-1)s(-1) and that of sodium azide as 2. 7x10(8)M(-1)s(-1). The present investigations show that vanillin can protect against photosensitization-induced ssbs in the plasmid pBR322 DNA, and this effect may partly be due to its ability to scavenge 1O(2).
authors.affiliation: Cell Biology Division, Bhabha Atomic Research Centre, 400 085, Mumbai, India.
authors.forename: S S
authors.initials: SS
authors.lastname: Kumar
authors.forename: A
authors.initials: A
authors.lastname: Ghosh
authors.forename: T P
authors.initials: TP
authors.lastname: Devasagayam
authors.forename: P S
authors.initials: PS
authors.lastname: Chauhan
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D001547:Benzaldehydes / Q000494:pharmacology*; D004249:DNA Damage; D005421:Flavoring Agents / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000494:pharmacology; D008027:Light; D008751:Methylene Blue / Q000633:toxicity*; D010100:Oxygen / Q000378:metabolism; D017319:Photosensitizing Agents / Q000633:toxicity*; D010957:Plasmids / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D026082:Singlet Oxygen
chemical_list: D000975:Antioxidants; D001547:Benzaldehydes; D005421:Flavoring Agents; D016166:Free Radical Scavengers; D017319:Photosensitizing Agents; D026082:Singlet Oxygen; C100058:vanillin; D010100:Oxygen; D008751:Methylene Blue