Hyaluronic acid/carboxymethylcellulose membrane surrounding an intraperitoneal or subcutaneous jejunojejunostomy in rats.
OBJECTIVE
To investigate the healing of intra-abdominal and extra-abdominal jejunal anastomoses that were surrounded by a hyaluronic acid/carboxymethylcellulose membrane (Seprafilm) in rats.


METHODS
Laboratory study.


METHODS
University hospital, The Netherlands.


METHODS
56 male Wistar rats.


METHODS
28 rats had jejunojejunostomies placed subcutaneously and 28 had them placed intra-abdominally. Half of each group of anastomoses were surrounded by Seprafilm.


METHODS
After 3 or 7 days, the anastomoses were tested for bursting pressure, tensile strength, and hydroxyproline concentrations.


RESULTS
There were numerous strong adhesions around the anastomoses in the subcutaneous position and Seprafilm had no influence on their extent. Bursting pressure and hydroxyproline concentrations were not affected by position or Seprafilm treatment. Tensile strength was significantly higher in the subcutaneous position (p < 0.01), but was unaffected by Seprafilm.


CONCLUSIONS
Anastomotic healing was not impaired in the subcutaneous or intraperitoneal position in this model. Seprafilm had no effect on the anastomosis.
van Oosterom
F J
FJ
Department of Surgery, Academic Medical Center at the University of Amsterdam, The Netherlands.
van Lanschot
J J
JJ
Oosting
J
J
Obertop
H
H
D000714:Anastomosis, Surgical; D000818:Animals; D002266:Carboxymethylcellulose Sodium; D006820:Hyaluronic Acid; D007582:Jejunostomy / Q000379:methods; D007583:Jejunum / Q000601:surgery; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012086:Reoperation; D014945:Wound Healing / Q000502:physiology*
D006820:Hyaluronic Acid; D002266:Carboxymethylcellulose Sodium