Molecular cloning and expression of aminopeptidase A isoforms from rat hippocampus.
The full-length cDNA encoding aminopeptidase A (APAL) was cloned from a rat hippocampus cDNA library. A short variant aminopeptidase A (APAS), produced by deletion, was also cloned. In the case of APAL, the longest open reading frame encodes 945 amino acid residues with a calculated molecular mass of 108 kDa, and the deduced amino acid sequence shows 76, 86 and 78% identity with its human, murine and porcine counterparts, respectively. Rat aminopeptidase A mRNAs were detected in the kidney, liver, heart and brain by Northern blot analysis. When overexpressed in COS-1 cells, APAL shows apparent aminopeptidase A activity, whereas APAS does not.
Lee
H J
HJ
Laboratory for Proteolytic Neuroscience, RIKEN Brain Science Institute, 2-1 Hirosawa, Wako-shi, 351-0198, Saitama, Japan. hahnjun@brain.riken.go.jp
Tomioka
M
M
Takaki
Y
Y
Masumoto
H
H
Saido
T C
TC
D000595:Amino Acid Sequence; D000626:Aminopeptidases / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry; D019556:COS Cells; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis; D015870:Gene Expression; D015723:Gene Library; D043384:Glutamyl Aminopeptidase; D006624:Hippocampus / Q000201:enzymology*; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D014162:Transfection
D018076:DNA, Complementary; D007527:Isoenzymes; D000626:Aminopeptidases; D043384:Glutamyl Aminopeptidase