Similar effects of electroporational stress and treatment with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate on vimentin expression in mouse plasmacytoma cells.
In mouse plasmacytoma cells (MPC-11), an activation of the normally repressed vimentin gene was observed as a response to transfectional stress. Effects of electroporation on vimentin gene expression were compared at the cellular and chromatin level to those caused by treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA). At the cellular level, similar changes in vimentin gene activity and cell-cycle distribution were observed by flow cytometry, whereas at the chromatin level similar changes in patterns of hypersensitive regions were detected by DNase I mapping. Additionally, a region located 700 bp upstream of the transcriptional start became hypersensitive to DNase I digestion upon electroporation and TPA treatment. This region overlaps two adjacent AP-1-like binding elements and generates specific DNA/AP-1 complexes in bandshift experiments. Therefore, the transcription factor AP-1 seems to play a central role in the activation of vimentin gene expression induced by these 2 different forms of stress.
Rauscher
A
A
Max-Planck-Institut für Zellbiologie, Rosenhof, D-68526, Ladenburg, Germany.
Giese
G
G
Nickel
J
J
Traub
P
P
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D003850:Deoxyribonuclease I; D018274:Electroporation; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D051379:Mice; D010954:Plasmacytoma; D010957:Plasmids; D015183:Restriction Mapping; D013755:Tetradecanoylphorbol Acetate; D018808:Transcription Factor AP-1 / Q000378:metabolism*; D014157:Transcription Factors; D014407:Tumor Cells, Cultured; D015854:Up-Regulation; D014746:Vimentin / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*
D018808:Transcription Factor AP-1; D014157:Transcription Factors; D014746:Vimentin; D003850:Deoxyribonuclease I; D013755:Tetradecanoylphorbol Acetate