Spermidine acetyltransferase is required to prevent spermidine toxicity at low temperatures in Escherichia coli.
Polyamines are required for optimal growth in most cells; however, polyamine accumulation leads to inhibition of cellular growth. To reduce intracellular polyamine levels, spermidine is monoacetylated in both prokaryotes and eukaryotes. In Escherichia coli, the speG gene encodes the spermidine acetyltransferase, which transfers the acetyl group to either the N-1 or N-8 position. In addition to polyamine accumulation, stress conditions, such as cold shock, cause an increase in the level of spermidine acetylation, suggesting an adaptive role for reduced polyamine levels under stressful growth conditions. The effect of spermidine accumulation on the growth of E. coli at low temperature was examined using a speG mutant. At 37 degrees C, growth of the speG mutant was normal in the presence of 0. 5 or 1 mM spermidine. However, following a shift to 7 degrees C, the addition of 0.5 or 1 mM spermidine resulted in inhibition of cellular growth or cell lysis, respectively. Furthermore, at 7 degrees C, spermidine accumulation resulted in a decrease in total protein synthesis accompanied by an increase in the synthesis of the major cold shock proteins CspA, CspB, and CspG. However, the addition of 50 mM Mg(2+) restored growth and protein synthesis in the presence of 0.5 mM spermidine. The results indicate that the level of spermidine acetylation increases at low temperature to prevent spermidine toxicity. The data suggest that the excess spermidine replaces the ribosome-bound Mg(2+), resulting in ribosome inactivation at low temperatures.
Limsuwun
K
K
Department of Microbiology, University of Georgia, Athens, Georgia 30602, USA.
Jones
P G
PG
D000123:Acetyltransferases / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism; D016601:RNA-Binding Proteins; D013095:Spermidine / Q000378:metabolism / Q000494:pharmacology*; D013696:Temperature; D000099228:Diamine N-Acetyltransferase
D000123:Acetyltransferases; D001426:Bacterial Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; D008274:Magnesium; D016601:RNA-Binding Proteins; D013095:Spermidine; D000099228:Diamine N-Acetyltransferase; C491179:CspB protein, E coli; C118134:CspG protein, E coli; C062260:cold shock protein CS7.4, Bacteria; C077188:cold-shock protein CspB, Bacteria