Sulfated fibroin, a novel sulfated peptide derived from silk, inhibits human immunodeficiency virus replication in vitro.
We prepared two kinds of sulfated silk fibroins, SclFib30 and SclFib31, which contain different amounts of sulfate. These sulfated silk fibroins have anti-HIV-1 activity in vitro, apparently due to interference with the adsorption of virus particles to CD4+ cells, and completely blocked virus binding to the cells at a concentration of 100 microg/ml. Sulfated fibroins also abolished cell-to-cell infection-induced syncytium formation upon cocultivation of MOLT-4 and MOLT-4/HIV-IIIB cells, suggesting that they would interfere with gp120 and prevent the formation of gp120/CD4 complex. Silk is used in biomaterials such as surgical sutures and is believed to be a safe material for humans. In accordance with low anticoagulant activity and high anti-HIV-1 activity against both X4 HIV-1 and R5 HIV-1 strains, sulfated silk fibroins have potential as antiviral material such for a vaginal anti-HIV formulation.
Gotoh
K
K
Department of Microbiology and Immunology, Kagoshima University Dental School, Japan.
Izumi
H
H
Kanamoto
T
T
Tamada
Y
Y
Nakashima
H
H
D000818:Animals; D019380:Anti-HIV Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000925:Anticoagulants / Q000378:metabolism; D012831:Bombyx; D002450:Cell Communication / Q000187:drug effects; D002460:Cell Line; D005260:Female; D005349:Fibroins / Q000302:isolation & purification / Q000494:pharmacology*; D015497:HIV-1 / Q000187:drug effects* / Q000472:pathogenicity / Q000502:physiology; D006801:Humans; D019476:Insect Proteins / Q000737:chemistry*; D047011:Silk; D013431:Sulfates / Q000378:metabolism; D013537:Sutures; D014621:Vagina / Q000821:virology; D014771:Virion; D014779:Virus Replication / Q000187:drug effects*
D019380:Anti-HIV Agents; D000925:Anticoagulants; D019476:Insect Proteins; D047011:Silk; D013431:Sulfates; D005349:Fibroins