Determination of stable isotopic enrichment and concentration of glycerol in plasma via gas chromatography-mass spectrometry for the estimation of lipolysis in vivo.
Measuring glycerol's rate of appearance into the plasma compartment provides an excellent estimation of whole-body lipolysis. The glycerol rate of appearance can be calculated by estimating the plasma dilution of continuously infused stable or radioactive isotopes of glycerol. Previously, determination of glycerol stable isotopic enrichment has required either chemical ionization gas chromatography-mass spectrometry (GC-MS) or electron impact ionization GC-MS in which a fragment containing only a portion of the glycerol molecule was measured. The present method uses tert.-butyldimethylsilyl (tBDMS) derivatization and electron impact ionization to measure a fragment including the entire glycerol molecule. The method determines concentration and enrichment of plasma glycerol in a simple, precise, and cost-efficient manner, providing a basis from which lipid homeostasis can be assessed.
Flakoll
P J
PJ
Department of Surgery, Vanderbilt University Medical Center, Nashville, TN 37232, USA. paul.flakoll@mcmail.vanderbilt.edu
Zheng
M
M
Vaughan
S
S
Borel
M J
MJ
D000818:Animals; D004285:Dogs; D004355:Drug Stability; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D005990:Glycerol / Q000097:blood*; D006801:Humans; D007554:Isotopes / Q000032:analysis / Q000097:blood; D008066:Lipolysis
D007554:Isotopes; D005990:Glycerol