[Use of a method of membraneless osmosis for concentrating proteins from molecularly dispersed and colloidally-dispersed solutions].
Theoretical principles, practical realizations, and future trends in the use of the method of protein concentration based on thermodynamic incompatibility of proteins with polysaccharides are reviewed. The relationship between structural features of these biopolymers (molecular weight, rigidity, and conformation of the polysaccharide chain; nature and ionogenic properties of its functional groups; the type of protein and state of its molecules), as well as major physicochemical parameters (pH, ionic strength, and temperature) and mechanical shift energy of the system on one hand and its phase diagram on the other hand, are discussed.
Antonov
Iu A
IuA
N.M. Emmanuel' Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, Russia. serg-ant@mtu-net.ru
D003102:Colloids; D008567:Membranes, Artificial; D009995:Osmosis; D011506:Proteins / Q000737:chemistry*; D012996:Solutions
D003102:Colloids; D008567:Membranes, Artificial; D011506:Proteins; D012996:Solutions