Enhanced chemiluminescent determination of chloramphenicol and related nitro compounds by 'on-line' photochemical reaction.
The viability of tandem photochemical reaction-chemiluminescence detection was studied for a heterogeneous family of nitro compounds using chloramphenicol as a test substance. The 'on-line' chemical photodegradation of chloramphenicol was performed in a flow injection assembly by using a photoreactor consisting of a 725 cm x 0.5 mm id piece of PTFE tubing coiled around an 8 W low-pressure mercury lamp. Photodegraded chloramphenicol was detected by oxidizing photo-fragments from the parent compound and their subsequent reaction with a luminol-Co(II) system. The calibration graph was linear up to 3 x 10(-5) mol l-1 chloramphenicol, the limit of detection was 3 x 10(-9) mol l-1, the relative standard deviation was 1.8% for 2 x 10(-6) mol l-1 of the drug and the sample throughput was 60 h-1. The proposed method was used to determine chloramphenicol in pharmaceutical formulations and its application to other related nitro compounds was studied.
David
V
V
Department of Analytical Chemistry, University of Bucharest, Romania.
Saèz
R M
RM
Mateo
J V
JV
Calatayud
J M
JM
D002138:Calibration; D002701:Chloramphenicol / Q000032:analysis*; D008163:Luminescent Measurements; D009574:Nitro Compounds / Q000032:analysis; D010777:Photochemistry; D012815:Signal Processing, Computer-Assisted
D009574:Nitro Compounds; D002701:Chloramphenicol