Animal-cell damage in sparged bioreactors.
The gas sparging of culture broth causes damage to suspended animal cells. However, despite this, sparged bioreactors remain the preferred means of cell culture because sparging is a robust method of supplying oxygen, especially on a large scale. This article examines the underlying mechanisms involved in bubble-associated cell damage and the methods available for controlling such damage.
Chisti
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Department of Chemical Engineering, University of Almería, E-04071, Almería, Spain. ychisti@hotmail.com
D000818:Animals; D019149:Bioreactors; D001769:Blood / Q000378:metabolism; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D003470:Culture Media; D007700:Kinetics; D008962:Models, Theoretical; D010100:Oxygen / Q000378:metabolism; D020442:Poloxamer / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000378:metabolism; D013501:Surface-Active Agents / Q000494:pharmacology
D003470:Culture Media; D013501:Surface-Active Agents; D020442:Poloxamer; D011092:Polyethylene Glycols; D010100:Oxygen