Sensitivity of NGF-responsive dorsal root ganglion neurons to semaphorin D is maintained in both neonatal and adult mice.
Using a coculture assay of DRG neurons and aggregates of cells transfected with individual semaphorins, we have investigated the ability of semaphorins A, D, and E to inhibit axonal growth from DRG neurons. We show that axons of these neurons that grow in response to NGF remain responsive to semaphorin D in neonatal and in adult mice, although sensitivity may decline in the latter. Consistent with these findings, expression of the semaphorin receptor, neuropilin-1, is maintained in the DRGs of adult mice.
Owesson
C
C
Randall Institute, King's College London, Drury Lane, WC2B 5RL, United Kingdom.
Pizzey
J
J
Tonge
D
D
D000818:Animals; D000831:Animals, Newborn; D001369:Axons / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D005727:Ganglia, Spinal / Q000187:drug effects* / Q000502:physiology; D006023:Glycoproteins / Q000494:pharmacology*; D051379:Mice; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D040121:Semaphorin-3A
D006023:Glycoproteins; D009414:Nerve Growth Factors; D040121:Semaphorin-3A