[Macaque immunization with virions purified from a primary isolate of the human immunodeficiency virus type 1 induced enhancement antibodies].
Six Rhesus macaques were hyperimmunized with either live infectious human immunodeficiency virus type 1 (HIV-1) or with beta-propiolactone--or formalin--inactivated HIV-1. The virus used was HIV-1 BX08, a primary virus isolate grown in human PBMC. Instead of eliciting virus-neutralizing antibodies, this regimen induced antibodies that enhanced HIV-1 infectivity for PBMC by 10 to 90 fold. Enhancement was also seen in a cell-to-cell fusion assay using a Semliki Forest virus replicon to express BX08 gp160 in CD4+, CCR5+ HeLa cell cultures. These observations raise the concern that whole virus particles-based HIV-1 vaccines might elicit enhancing antibodies that could play a facilitating role in the transmission and/or evolution of the disease.
Verrier
F
F
Unité de Virologie Moléculaire, URA CNRS 1966, Institut Pasteur, Paris.
Moog
C
C
Barre-Sinoussi
F
F
Van der Ryst
E
E
Spenlehauer
C
C
Girard
M
M
D000818:Animals; D019067:Antibody-Dependent Enhancement / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D007114:Immunization; D008251:Macaca / Q000276:immunology*; D008297:Male; D014771:Virion / Q000302:isolation & purification*