title: White spot syndrome virus (WSSV) in cultured Penaeus monodon in the Philippines.
abstract: The prevalence and geographic distribution of white spot syndrome virus (WSSV) infection among cultured penaeid shrimp in the Philippines was determined from January to May, 1999, using PCR (polymerase chain reaction) protocol and Western blot assays. A total of 71 samples consisting of 18 post-larvae (PL) and 53 juvenile/adult shrimp samples (56 to 150 days-of-culture, DOC) were screened for WSSV. Of the 71 samples tested, 51 (72%) were found positive for WSSV by PCR: 61% (31/51) after 1-step PCR and 39% (20/51) after 2-step, non-nested PCR. Of the PL and juvenile/adult shrimp samples tested, 50 and 79% were positive for WSSV, respectively. By Western blot, only 6 of the 51 (12%) PCR-positive samples tested positive for WSSV. Of the 20 samples negative for WSSV by PCR, all tested negative for WSSV by Western blot assay. This is the first report of the occurrence of WSSV in the Philippines.
authors.affiliation: National Institute of Molecular Biology and Biotechnology (BIOTECH), University of the Philippines-Los Baños, College, Philippines.
authors.forename: F O
authors.initials: FO
authors.lastname: Magbanua
authors.forename: K T
authors.initials: KT
authors.lastname: Natividad
authors.forename: V P
authors.initials: VP
authors.lastname: Migo
authors.forename: C G
authors.initials: CG
authors.lastname: Alfafara
authors.forename: F O
authors.initials: FO
authors.lastname: de la Peña
authors.forename: R O
authors.initials: RO
authors.lastname: Miranda
authors.forename: J D
authors.initials: JD
authors.lastname: Albaladejo
authors.forename: E C
authors.initials: EC
authors.lastname: Nadala
authors.forename: P C
authors.initials: PC
authors.lastname: Loh
authors.forename: L
authors.initials: L
authors.lastname: Mahilum-Tapay
mesh_terms: D000818:Animals; D017756:Aquaculture; D015153:Blotting, Western / Q000662:veterinary; D004267:DNA Viruses; D033561:Penaeidae / Q000821:virology*; D010679:Philippines; D016133:Polymerase Chain Reaction / Q000662:veterinary