Equivalence of assurance Gold Enzyme Immunoassay for visual or instrumental detection of motile and nonmotile Salmonella in all foods to AOAC culture method: collaborative study.
Six foods representative of a wide variety of processed, dried powder processed, and raw food types were analyzed by the Assurance Gold Salmonella Enzyme Immunoassay (EIA) and AOAC INTERNATIONAL culture method. Paired samples of each food type were simultaneously analyzed; one sample by the Assurance method and one by the AOAC culture method. The results for Assurance method were read visually and instrumentally with a microplate reader. A total of 24 laboratories representing federal government agencies and private industry, in the United States and Canada, participated in this collaborative study. Food types were inoculated with species of Salmonella with the exception of raw ground chicken, which was naturally contaminated. No statistical differences (p < 0.05) were observed between Assurance Gold Salmonella EIA with either visual or instrumental interpretation and the AOAC culture method for any inoculation level of any food type or naturally contaminated food. The Assurance visual and instrumental options of reading sample reactions produced the same results for 1277 of the 1296 sample and controls analyzed.
Feldsine
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BioControl Systems, Inc., Bellevue, WA 98005, USA.
Mui
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Forgey
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Kerr
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D000818:Animals; D002099:Cacao / Q000382:microbiology; D002170:Canada; D002645:Chickens; D004531:Eggs / Q000382:microbiology; D005516:Food Microbiology; D006077:Government Agencies; D007054:Ice Cream / Q000382:microbiology; D007124:Immunoenzyme Techniques; D007753:Laboratories; D008460:Meat / Q000382:microbiology; D008892:Milk / Q000382:microbiology; D011202:Poultry Products / Q000382:microbiology; D011786:Quality Control; D012475:Salmonella / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013552:Swine; D014481:United States