Alpha1-antitrypsin in serum determined by capillary isoelectric focusing.
A capillary isoelectric focusing (CIEF) method using bare fused-silica capillaries filled with polyethylene oxide (PEO) and carrier ampholyte solutions in the pH 3.5-5.0 range has been developed for the identification of alpha1-antitrypsin (alpha1AT) phenotypes in human serum. This novel procedure was routinely applied to the study of serum samples of five controls whose alpha1AT phenotype was previously identified and of twelve subjects whose alpha1AT phenotype was unknown. The results obtained allowed us to confirm or identify the alpha1AT phenotype in all sera tested. This procedure seems particularly suitable for identification of alpha1AT variants associated with diseases of clinical relevance.
Lupi
A
A
Dipartimento di Biochemica A. Castellani, Università di Pavia, Italy.
Viglio
S
S
Luisetti
M
M
Gorrini
M
M
Coni
P
P
Faa
G
G
Cetta
G
G
Iadarola
P
P
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D019075:Electrophoresis, Capillary / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D014644:Genetic Variation; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods; D019272:Leukocyte Elastase / Q000037:antagonists & inhibitors; D010641:Phenotype; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D000515:alpha 1-Antitrypsin / Q000032:analysis / Q000235:genetics*
D000515:alpha 1-Antitrypsin; D019272:Leukocyte Elastase