Antioxidative efficacy of parallel and combined supplementation with coenzyme Q10 and d-alpha-tocopherol in mildly hypercholesterolemic subjects: a randomized placebo-controlled clinical study.
It has been claimed that coenzyme Q10 (Q10) would be an effective plasma antioxidant since it can regenerate plasma vitamin E. To test separate effects and interaction between Q10 and vitamin E in the change of plasma concentrations and in the antioxidative efficiency, we carried out a double-masked, double-blind clinical trial in 40 subjects with mild hypercholesterolemia undergoing statin treatment. Subjects were randomly allocated to parallel groups to receive either Q10 (200 mg daily), d-alpha-tocopherol (700 mg daily), both antioxidants or placebo for 3 months. In addition we investigated the pharmacokinetics of Q10 in a separate one-week substudy. In the group that received both antioxidants, the increase in plasma Q10 concentration was attenuated. Only vitamin E supplementation increased significantly the oxidation resistance of isolated LDL. Simultaneous Q10 supplementation did not increase this antioxidative effect of vitamin E. Q10 supplementation increased and vitamin E decreased significantly the proportion of ubiquinol of total Q10, an indication of plasma redox status in vivo. The supplementations used did not affect the redox status of plasma ascorbic acid. In conclusion, only vitamin E has antioxidative efficiency at high radical flux ex vivo. Attenuation of the proportion of plasma ubiquinol of total Q10 in the vitamin E group may represent in vivo evidence of the Q10-based regeneration of the tocopheryl radicals. In addition, Q10 might attenuate plasma lipid peroxidation in vivo, since there was an increased proportion of plasma ubiquinol of total Q10.
Kaikkonen
J
J
Research Institute of Public Health, University of Kuopio, Finland.
Nyyssönen
K
K
Tomasi
A
A
Iannone
A
A
Tuomainen
T P
TP
Porkkala-Sarataho
E
E
Salonen
J T
JT
D000368:Aged; D000975:Antioxidants / Q000008:administration & dosage*; D001205:Ascorbic Acid / Q000097:blood; D003067:Coenzymes; D019587:Dietary Supplements; D004311:Double-Blind Method; D004347:Drug Interactions; D005260:Female; D006801:Humans; D006937:Hypercholesterolemia / Q000188:drug therapy*; D015227:Lipid Peroxidation / Q000187:drug effects; D008077:Lipoproteins, LDL / Q000097:blood; D008297:Male; D008875:Middle Aged; D010919:Placebos; D014451:Ubiquinone / Q000008:administration & dosage* / Q000031:analogs & derivatives / Q000493:pharmacokinetics / Q000627:therapeutic use; D014527:Uric Acid / Q000097:blood; D014810:Vitamin E / Q000008:administration & dosage* / Q000627:therapeutic use
D000975:Antioxidants; D003067:Coenzymes; D008077:Lipoproteins, LDL; D010919:Placebos; C099252:oxidized low density lipoprotein; D014451:Ubiquinone; D014810:Vitamin E; D014527:Uric Acid; C024989:coenzyme Q10; D001205:Ascorbic Acid