Protein phosphatase inhibition assay adapted for determination of total DSP in contaminated mussels.
The fluorescence protein phosphatase (PP-2A) inhibition assay detects okadaic acid (OA) and DTX-1 in mussels down to 1 microg/100 g of mussel tissue. It is more sensitive than the mouse bioassay (detection limit, 20 microg/100 g) or ELISA using the SCETI DSP check kit (detection limit, 10 microg/100 g). A drawback of the PP-2A assay method has been its lack of sensitivity towards the ester derivatives of OA and DTX-1. This has been addressed by including a hydrolysis step in the pretreatment of extracts which allows these derivatives to be converted to either okadaic acid or DTX-1 prior to the DSP assay. The method has been applied to the analysis of DSP in 19 samples of naturally contaminated mussels and the results from the PP-2A inhibition assay compared to those for HPLC. A good correlation was obtained for OA determined by the two methods in both unhydrolysed and hydrolysed samples. The new procedure will substantially reduce the incidence of false negatives in the DSP assay.
Mountfort
D O
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Cawthron Institute, Private Bag 2, Nelson, New Zealand. doug@cawtron.org.nz
Suzuki
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T
Truman
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D000818:Animals; D049872:Bivalvia; D002273:Carcinogens / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005506:Food Contamination / Q000032:analysis*; D019319:Okadaic Acid / Q000031:analogs & derivatives / Q000032:analysis*; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors*; D012680:Sensitivity and Specificity
D002273:Carcinogens; D019319:Okadaic Acid; D010749:Phosphoprotein Phosphatases