title: Mice mutant for Ppap2c, a homolog of the germ cell migration regulator wunen, are viable and fertile.
abstract: Phosphatidic acid phosphatases (PAPs) catalyze the conversion of phosphatidic acid to diacylglycerol and inorganic phosphate and have been postulated to function both in lipid biosynthesis and in cellular signal transduction. In Drosophila melanogaster, the Type 2 phosphatidic acid phosphatase protein encoded by the wunen gene, negatively regulates primordial germ cell migration. We recently described the cloning and characterization of the mouse Ppap2c gene, which encodes the Type 2 phosphatidic acid phosphatase Pap2c (Zhang et al., Genomics 63:142-144). To analyze the in vivo role of the Ppap2c gene we constructed a null mutation by gene targeting. Ppap2c(-/-) homozygous mutant mice were viable, fertile, and exhibited no obvious phenotypic defects. These data demonstrate that the Ppap2c gene is not essential for embryonic development or fertility in mice.
authors.affiliation: The Jackson Laboratory, Bar Harbor, Maine 04609, USA.
authors.forename: N
authors.initials: N
authors.lastname: Zhang
authors.forename: J P
authors.initials: JP
authors.lastname: Sundberg
authors.forename: T
authors.initials: T
authors.lastname: Gridley
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000235:genetics*; D002478:Cells, Cultured; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics; D005298:Fertility / Q000235:genetics*; D018390:Gene Targeting; D005854:Germ Cells / Q000201:enzymology / Q000502:physiology*; D019476:Insect Proteins / Q000235:genetics*; D008565:Membrane Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D008822:Mice, Transgenic; D010711:Phosphatidate Phosphatase / Q000172:deficiency* / Q000235:genetics*; D017384:Sequence Deletion; D012689:Sequence Homology, Nucleic Acid
chemical_list: D029721:Drosophila Proteins; D019476:Insect Proteins; D008565:Membrane Proteins; C406242:phosphatidic acid phosphatase type 2; D010711:Phosphatidate Phosphatase; C103065:wun protein, Drosophila