Measuring gene expression by quantitative proteome analysis.
Proteome analysis is most commonly accomplished by the combination of two-dimensional gel electrophoresis for protein separation, visualization, and quantification and mass spectrometry for protein identification. Over the past year, exceptional progress has been made towards developing a new technology base for the precise quantification and identification of proteins in complex mixtures, that is, quantitative proteomics.
Gygi
S P
SP
Department of Molecular Biotechnology, University of Washington, Seattle 98195-7730, USA. gygi@u.washington.edu
Rist
B
B
Aebersold
R
R
D001671:Biochemistry / Q000379:methods*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D020869:Gene Expression Profiling / Q000379:methods*; D007554:Isotopes; D013058:Mass Spectrometry / Q000379:methods; D011506:Proteins / Q000032:analysis* / Q000235:genetics*
D007554:Isotopes; D011506:Proteins