title: Evaluation of a capture screening enzyme-linked immunosorbent assay for combined determination of immunoglobulin M and G antibodies produced during Dengue infection.
abstract: A commercially available enzyme-linked immunosorbent assay (ELISA) (PanBio Dengue Screening ELISA) that utilized both immunoglobulin M (IgM) and IgG capture in the same microtiter well for the diagnosis of dengue infection was evaluated. Sensitivity in primary and secondary dengue was 95%, while specificity was 94%.
authors.affiliation: WHO Collaborating Centre for Arbovirus Reference and Research (Dengue and Dengue Haemorrhagic Fever), Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. lamsk@medicine.med.um.edu.my
authors.forename: S
authors.initials: S
authors.lastname: Kit Lam
authors.forename: C
authors.initials: C
authors.lastname: Lan Ew
authors.forename: J L
authors.initials: JL
authors.lastname: Mitchell
authors.forename: A J
authors.initials: AJ
authors.lastname: Cuzzubbo
authors.forename: P L
authors.initials: PL
authors.lastname: Devine
mesh_terms: D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D003715:Dengue / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005069:Evaluation Studies as Topic; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology; D007075:Immunoglobulin M / Q000097:blood* / Q000276:immunology; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011933:Reagent Kits, Diagnostic