FAST slides: a novel surface for microarrays.
We have evaluated FAST slides, a glass slide with a microporous polymeric surface that is a suitable substrate for microarray technology. The surface is a nitrocellulose-based polymer that binds DNA and proteins in a noncovalent but irreversible manner. FAST slides are compatible with robotic systems currently used to create microarrays and can easily accommodate volumes of 0.03-2 nL/spot. Our data indicate that FAST slides have a much higher binding capacity for DNA and better spot-to-spot consistency than traditional poly-lysine-coated slides. In addition, FAST slides are well suited for fluorescent detection because of their relatively low light scatter and efficient retention of arrayed DNA. These properties translate into fluorescent sensitivity comparable to modified glass surfaces. FAST slides are also ideal for arraying proteins, making them the only substrate of their kind currently available for microarray applications.
Stillman
B A
BA
Schleicher & Schuell, Keene, NH, USA.
Tonkinson
J L
JL
D019921:Biotinylation; D003101:Collodion; D004247:DNA / Q000032:analysis; D005456:Fluorescent Dyes; D005898:Glass; D008027:Light; D008163:Luminescent Measurements; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D011107:Polylysine; D011108:Polymers; D012542:Scattering, Radiation
D005456:Fluorescent Dyes; D011108:Polymers; D011107:Polylysine; D003101:Collodion; D004247:DNA