title: HPLC analysis of flavonoids and secoiridoids in leaves of Ligustrum vulgare L. (Oleaceae).
abstract: Identification and quantification of flavonol glycosides and secoiridoids was carried out on leaves of Ligustrum vulgare L. (Oleaceae) by means of HPLC-DAD and HPLC-MS analysis. In addition to previously reported secoiridoids (oleuropein, ligustaloside A, ligustaloside B, and ligstroside) four kaempferol glycosides (kaempferol 3-O-glucoside 7-O-rhamnoside, kaempferol 3, 7-O-dirhamnoside, kaempferol 3-O-rhamnoside, and kaempferol 3-O-glucoside) and two quercetin glycosides (quercetin 3-O-glucoside 7-O-rhamnoside and quercetin 3,7-O-dirhamnoside) were present in leaves of L. vulgare L. Although secoiridoids accounted for nearly the 76% of the total leaf polyphenols content (with ligustaloside A as the main component), kaempferol glycosides were also accumulated in the leaves of L. vulgare L. to a relatively high extent (23%). Contribution of quercetin derivatives was minor under our experimental conditions. Our findings suggest that flavonol glycosides may have a central role in both the ecology and the biology of L. vulgare L.
authors.affiliation: Dipartimento di Scienze Farmaceutiche, Università di Firenze, Via Gino Capponi 9, I-50100 Florence, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Romani
authors.forename: P
authors.initials: P
authors.lastname: Pinelli
authors.forename: N
authors.initials: N
authors.lastname: Mulinacci
authors.forename: F F
authors.initials: FF
authors.lastname: Vincieri
authors.forename: E
authors.initials: E
authors.lastname: Gravano
authors.forename: M
authors.initials: M
authors.lastname: Tattini
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005419:Flavonoids / Q000032:analysis*; D005960:Glucosides / Q000032:analysis*; D039823:Iridoids; D013058:Mass Spectrometry; D018515:Plant Leaves / Q000737:chemistry; D010944:Plants / Q000737:chemistry*; D011714:Pyrans / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005419:Flavonoids; D005960:Glucosides; D039823:Iridoids; D011714:Pyrans