title: Prevalence of non-O157:H7 shiga toxin-producing Escherichia coli in diarrheal stool samples from Nebraska.
abstract: We determined the prevalence of Shiga toxin-producing Escherichia coli (STEC) in diarrheal stool samples from Nebraska by three methods: cefixime-tellurite sorbitol MacConkey (CT- SMAC) culture, enterohemorrhagic E. coli (EHEC) enzyme immunoassay, and stx1,2 polymerase chain reaction (PCR). Fourteen (4.2%) of 335 specimens were positive by at least one method (CT-SMAC culture [6 of 14], EHEC enzyme immunoassay [13 of 14], stx1,2 PCR [14 of 14]). Six contained serogroup O157, while non-O157 were as prevalent as O157 serogroups.
authors.affiliation: University of Nebraska Medical Center, Omaha, Nebraska 68198-5400, USA.
authors.forename: P D
authors.initials: PD
authors.lastname: Fey
authors.forename: R S
authors.initials: RS
authors.lastname: Wickert
authors.forename: M E
authors.initials: ME
authors.lastname: Rupp
authors.forename: T J
authors.initials: TJ
authors.lastname: Safranek
authors.forename: S H
authors.initials: SH
authors.lastname: Hinrichs
mesh_terms: D003967:Diarrhea / Q000453:epidemiology* / Q000382:microbiology*; D019453:Escherichia coli O157 / Q000145:classification* / Q000378:metabolism / Q000472:pathogenicity; D005243:Feces / Q000382:microbiology; D006801:Humans; D007124:Immunoenzyme Techniques; D009329:Nebraska / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012703:Serotyping / Q000379:methods*; D022621:Shiga Toxin / Q000096:biosynthesis / Q000302:isolation & purification*
chemical_list: D022621:Shiga Toxin