title: Implantation of bone marrow beneath the kidney capsule results in transfer not only of functional stroma but also of hematopoietic repopulating cells.
abstract: Renal ossicles are ossified structures developed after the implantation of a bone marrow (BM) plug beneath the kidney capsule. The authors have investigated the origin of the hematopoietic cells in murine renal ossicles by conducting sex-mismatched implants into Ly-5 congenic mice. BM plugs from transgenic mice provided additional genotypic tracers. Flow cytometry analyses on nonadherent cells from long-term cultures established with ossicles excised at 17 to 40 weeks postimplantation evidenced the presence of 5% to 70% of donor-derived myeloid cells. The genetic analysis of the day 12 colony-forming unit (CFU-S(12)) population in ossicles excised at 10 to 40 weeks postimplantation revealed that 16% to 93% of the colonies were of donor origin. Moreover, we describe for the first time the presence of long-term repopulating cells of donor origin in ossicles excised at 10 to 19 weeks postimplantation.
authors.affiliation: Departamento de Biología Molecular y Celular y Terapia Génica, Centro de Investigaciones Energéticas, Madrid, Spain. florencio.varas@ciemat.es
authors.forename: F
authors.initials: F
authors.lastname: Varas
authors.forename: T
authors.initials: T
authors.lastname: Grande
authors.forename: A
authors.initials: A
authors.lastname: Ramírez
authors.forename: J A
authors.initials: JA
authors.lastname: Bueren
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000473:pathology* / Q000502:physiology; D007668:Kidney / Q000473:pathology* / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D017154:Stromal Cells / Q000473:pathology* / Q000502:physiology