title: Isolation of a lethal rhabdovirus from the cultured Chinese sucker Myxocyprinus asiaticus.
abstract: A rhabdovirus was found to be associated with a lethal hemorrhagic disease in the cultured Chinese sucker Myxocyprinus asiaticus Bleeker. The rhabdovirus was amplified and isolated from the infected GCO (grass carp ovary) cells. In ultrathin sections of liver cells from the diseased fish, the virus particles exhibited the characteristic bacilliform morphology, and budded through vesicle membranes of the infected cells. The isolated rhabdovirus particles were found to have a bacilliform morphology with 2 rounded ends rather than a typical flat base. The virus particles were measured and ranged in size from 150 to 200 nm in length and 50 to 60 nm in diameter. Most other characteristics, including their size, extensive virus infectivity to fish cell lines, strong cytopathogenic effects, stability at high temperatures, vesicle formation in infected cells, structure protein electrophoretic patterns and the presence of an RNA genome, very closely resembled those of other fish rhabdoviruses. At present it is not known if this is a novel virus species or if it is an isolate of a known fish rhabdovirus. Until a confirmed identification can be made, we will temporarily refer to this virus as Chinese sucker rhabdovirus (CSRV).
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, PR China. jfgui@ihb.ac.cn
authors.forename: Q Y
authors.initials: QY
authors.lastname: Zhang
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Li
authors.forename: J F
authors.initials: JF
authors.lastname: Gui
mesh_terms: D000818:Animals; D017756:Aquaculture; D002460:Cell Line; D003531:Cypriniformes; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D005393:Fish Diseases / Q000473:pathology / Q000821:virology*; D008099:Liver / Q000473:pathology; D012209:Rhabdoviridae / Q000302:isolation & purification* / Q000472:pathogenicity; D018353:Rhabdoviridae Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D013696:Temperature