title: Transient IFN-gamma synthesis in the lymph node draining a dermal site loaded with UV-irradiated herpes simplex virus type 1: an NK- and CD3-dependent process regulated by IL-12 but not by IFN-alpha/beta.
abstract: Our previous studies have shown that UV-inactivated, non-replicating herpes simplex virus type 1 (UV-HSV-1) triggers early and transient synthesis of IFN-alpha/beta in the mouse regional lymph node when delivered upstream (i.e. in the ear dermis). In this study, it is demonstrated, by use of a quantitative RT-PCR readout assay, that IFN-gamma mRNA expression was rapidly and transiently upregulated in draining lymph nodes when UV-HSV-1 was delivered in the ear dermis of C57Bl/6 mice. An increased number of IFN-gamma-producing cells was also detected in the lymph node by flow cytometric analysis. Two different subsets of cells, namely DX5(+) NK cells and CD3epsilon(+) T cells, accounted for this early IFN-gamma synthesis. Prompt upregulation of IFN-alpha and IL-12p40 mRNA was also recorded. We took advantage of IFN-alpha/beta-receptor knockout and wild-type 129 mice to study a potential role of IFN-alpha/beta in the signalling pathway leading to IFN-gamma transcription/translation. IFN-gamma mRNA upregulation still occurred in IFN-alpha/beta-receptor(-/-) mice, showing that IFN-alpha/beta was dispensable. The use of IL-12-neutralizing antibodies, prior to UV-HSV-1 delivery, confirmed the major role played by IL-12 in the early/transient IFN-gamma burst.
authors.affiliation: Unité de Virologie et Immunologie Moléculaires, Institut National de la Recherche Agronomique, F-78352 Jouy-en-Josas cedex, France1.
authors.forename: S
authors.initials: S
authors.lastname: Riffault
authors.affiliation: Unité de Virologie et Immunologie Moléculaires, Institut National de la Recherche Agronomique, F-78352 Jouy-en-Josas cedex, France1.
authors.forename: C
authors.initials: C
authors.lastname: Carrat
authors.affiliation: Unité d'Immunophysiologie et Parasitisme Intracellulaire, Institut Pasteur, Paris, France2.
authors.forename: G
authors.initials: G
authors.lastname: Milon
authors.affiliation: Unité de Virologie et Immunologie Moléculaires, Institut National de la Recherche Agronomique, F-78352 Jouy-en-Josas cedex, France1.
authors.forename: B
authors.initials: B
authors.lastname: Charley
authors.affiliation: Unité d'Immunophysiologie et Parasitisme Intracellulaire, Institut Pasteur, Paris, France2.
authors.forename: J H
authors.initials: JH
authors.lastname: Colle
mesh_terms: D000818:Animals; D017252:CD3 Complex / Q000378:metabolism*; D005260:Female; D018259:Herpesvirus 1, Human / Q000276:immunology / Q000528:radiation effects*; D006801:Humans; D016898:Interferon-alpha / Q000502:physiology*; D016899:Interferon-beta / Q000502:physiology*; D007371:Interferon-gamma / Q000096:biosynthesis*; D018664:Interleukin-12 / Q000502:physiology*; D007694:Killer Cells, Natural / Q000378:metabolism* / Q000821:virology; D008198:Lymph Nodes / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D012867:Skin / Q000378:metabolism*; D014466:Ultraviolet Rays; D015854:Up-Regulation
chemical_list: D017252:CD3 Complex; D016898:Interferon-alpha; D012333:RNA, Messenger; D018664:Interleukin-12; D016899:Interferon-beta; D007371:Interferon-gamma