title: Molecular cloning and expression of a phospholipid hydroperoxide glutathione peroxidase homolog in Oryza sativa.
abstract: A cDNA encoding putative phospholipid hydroperoxide glutathione peroxidase (PHGPX) was isolated from rice using rapid amplification of cDNA ends. This cDNA, designated ricPHGPX, includes an open reading frame encoding a protein of 169 amino acids which shares about 60% and 50% amino acid sequence identity with plant and mammalian PHGPXs, respectively. The gene is expressed at a relative high level in flag leaves and the expression can be markedly induced by oxidative stress, suggesting that the product of the gene plays a key role in defense against oxidative damage in rice.
authors.affiliation: Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, PR China. liujy@mail.tsinghua.edu.cn
authors.forename: W J
authors.initials: WJ
authors.lastname: Li
authors.forename: H
authors.initials: H
authors.lastname: Feng
authors.forename: J H
authors.initials: JH
authors.lastname: Fan
authors.forename: R Q
authors.initials: RQ
authors.lastname: Zhang
authors.forename: N M
authors.initials: NM
authors.lastname: Zhao
authors.forename: J Y
authors.initials: JY
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D005786:Gene Expression Regulation; D017343:Genes, Plant; D005979:Glutathione Peroxidase / Q000235:genetics*; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology / Q000235:genetics*; D018384:Oxidative Stress; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D016415:Sequence Alignment; D017385:Sequence Homology
chemical_list: D018076:DNA, Complementary; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D005979:Glutathione Peroxidase