In vitro effects of corticosteroids, DDT, and 4, 9-dichlorodibenzodioxin on rat liver xanthine oxidase activity. Interactions between xanthine oxidase and cytochrome P450 in rat liver in vivo.
It was established that deoxycorticosterone, cortisol, dexamethasone, DDT, and 4,9-dichlorodibenzodioxin inhibit in vitro binding of xanthine to highly purified rat liver xanthine oxidase. They are suggested to be allosteric inhibitors. The corresponding inhibition and binding constants were estimated. Also established was that cortisol and DDT, in dose 20 mg per kg of weight, inhibit xanthine oxidase and increase microsomal cytochrome P450 level in rat liver. There is an inverse relationship between xanthine oxidase activity and cytochrome P450 level in rat liver.
Sumbayev
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Department of Biochemistry, Mechnikov Odessa State University, Odessa, 270058, Ukraine. inna@farlep.net
D000305:Adrenal Cortex Hormones / Q000494:pharmacology; D000818:Animals; D065607:Cytochrome P-450 Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D003634:DDT / Q000494:pharmacology; D004147:Dioxins / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005260:Female; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors* / Q000378:metabolism
C415444:4,9-dichlorodibenzodioxin; D000305:Adrenal Cortex Hormones; D065607:Cytochrome P-450 Enzyme Inhibitors; D004147:Dioxins; D004791:Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System; D003634:DDT; D014969:Xanthine Oxidase