title: Characterization and affinity purification of juvenile hormone esterase from Bombyx mori.
abstract: Juvenile hormone esterase (JHE) from hemolymph of the silkworm moth Bombyx mori was characterized for substrate specificity and inhibitor sensitivity. B. mori JHE hydrolyzed the juvenile hormone surrogate substrate methyl n-heptylthioacetothioate (HEPTAT) more efficiently than p-nitrophenyl acetate and 1-naphthyl acetate substrates widely used to assay total carboxylesterase activity. B. mori JHE was sensitive to 3-octylthio-1,1,1-trifluoro-2-propanone (OTFP), which was developed as a selective inhibitor for lepidopteran JHE, and relatively insensitive to diisopropyl fluorophosphate (DFP), an inhibitor of serine esterases but not of all JHEs. Affinity purification with a trifluoromethyl ketone ligand was more efficient for purification of B. mori JHE than DEAE ion exchange chromatography.
authors.affiliation: Department of Insect Physiology and Behavior, National Institute of Sericultural and Entomological Science, Tsukuba, Ibaraki, Japan. shiotsuk@nises.affrc.go.jp
authors.forename: T
authors.initials: T
authors.lastname: Shiotsuki
authors.forename: B C
authors.initials: BC
authors.lastname: Bonning
authors.forename: M
authors.initials: M
authors.lastname: Hirai
authors.forename: K
authors.initials: K
authors.lastname: Kikuchi
authors.forename: B D
authors.initials: BD
authors.lastname: Hammock
mesh_terms: D000085:Acetates / Q000378:metabolism; D000096:Acetone / Q000031:analogs & derivatives / Q000378:metabolism; D000818:Animals; D012831:Bombyx / Q000201:enzymology*; D002265:Carboxylic Ester Hydrolases / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D006458:Hemolymph / Q000737:chemistry; D008024:Ligands; D013379:Substrate Specificity
chemical_list: D000085:Acetates; D008024:Ligands; C093097:methyl 1-heptylthioacetothioate; D000096:Acetone; C055697:3-octylthio-1,1,1-trifluoro-2-propanone; D002265:Carboxylic Ester Hydrolases; C038003:juvenile hormone esterase