title: The paramyxovirus simian virus 5 V protein slows progression of the cell cycle.
abstract: Infection of cells by many viruses affects the cell division cycle of the host cell to favor viral replication. We examined the ability of the paramyxovirus simian parainfluenza virus 5 (SV5) to affect cell cycle progression, and we found that SV5 slows the rate of proliferation of HeLa T4 cells. The SV5-infected cells had a delayed transition from G(1) to S phase and prolonged progression through S phase, and some of the infected cells were arrested in G(2) or M phase. The levels of p53 and p21(CIP1) were not increased in SV5-infected cells compared to mock-infected cells, suggesting that the changes in the cell cycle occur through a p53-independent mechanism. However, the phosphorylation of the retinoblastoma protein (pRB) was delayed and prolonged in SV5-infected cells. The changes in the cell cycle were also observed in cells expressing the SV5 V protein but not in the cells expressing the SV5 P protein or the V protein lacking its unique C terminus (VDeltaC). The unique C terminus of the V protein of SV5 was shown previously to interact with DDB1, which is the 127-kDa subunit of the multifunctional damage-specific DNA-binding protein (DDB) heterodimer. The coexpression of DDB1 with V can partially restore the changes in the cell cycle caused by expression of the V protein.
authors.affiliation: Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, Illinois 60208, USA.
authors.forename: G Y
authors.initials: GY
authors.lastname: Lin
authors.forename: R A
authors.initials: RA
authors.lastname: Lamb
mesh_terms: D002453:Cell Cycle; D006367:HeLa Cells / Q000473:pathology / Q000821:virology*; D006801:Humans; D018108:Respirovirus; D015678:Viral Structural Proteins
chemical_list: C097019:V protein, Simian parainfluenza virus 5; D015678:Viral Structural Proteins