Insulin receptor and insulin receptor substrate-I in rat retinae.
Insulin receptor substrate-I (IRS-I) is a major cytosolic substrate of the insulin receptor Expression of insulin receptor and IRS-I, and the distribution of these components of the insulin-signalling pathway, were investigated in rat retinae. Insulin receptor and IRS-I were located in retinal sections with anti-insulin receptor and anti-IRS-I antibodies. Reverse transcription-polymerase chain reaction (RT-PCR) of retinal mRNA was performed with primers specific for insulin receptor and IRS-I gene sequences. Immunohistochemistry demonstrated distinct but closely associated staining patterns for insulin receptor and IRS-I throughout rat retinae. The RT-PCR product from rat retinal insulin receptor mRNA corresponded to the high affinity insulin receptor isoform. The RT-PCR product for retinal IRS-I mRNA agreed with that predicted from the gene sequence. The expression of IRS- I and insulin receptors indicates a signalling mechanism by which insulin can influence retinal metabolism or function.
Gosbell
A D
AD
Department of Surgery, Monash University, Clayton, Victoria, Australia. Andrew.Gosbell@med.monash.edu.au
Favilla
I
I
Baxter
K M
KM
Jablonski
P
P
D000818:Animals; D017931:DNA Primers / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D015870:Gene Expression; D007124:Immunoenzyme Techniques; D055504:Insulin Receptor Substrate Proteins; D008297:Male; D010750:Phosphoproteins / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D020318:Rats, Long-Evans; D011972:Receptor, Insulin / Q000096:biosynthesis / Q000235:genetics*; D012160:Retina / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology
D017931:DNA Primers; D055504:Insulin Receptor Substrate Proteins; C526296:Irs1 protein, rat; D010750:Phosphoproteins; D012333:RNA, Messenger; D011972:Receptor, Insulin