Comparison of amino acid sequences at the amino acid 130-160 region of VP1 polypeptide of Indian field isolates of foot-and-mouth disease virus serotype Asia 1.
The nucleotide and deduced amino acid sequences in the amino acid (aa) 130-160 region of VP1 polypeptide of 65 field isolates of foot- and mouth disease virus (FMDV) serotype Asia 1 were determined and the consensus sequences were deduced. Comparison of amino acid sequences revealed conservation of NGK (130-132), TYG (134-136), RGD (142-144), and LPTSF (156-160) motifs and aa 148 (L) while variation was observed at the rest of the region (variability index (VI) of 2.06 to 16.85). Synonymous and non-synonymous mutations at the nucleotide level were well correlated with those of the corresponding amino acids. Comparison of the aa 130-160 sequence of Asia 1 serotype with those of other serotypes of FMDV revealed conservation of aa 135, 148-149, 157 and 160. Amino acids 133-138 and 148-154 were unique for Asia 1 serotype and are presumably responsible for its distinct antigenic nature. The present study revealed that the FMDV isolates of serotype Asia 1 causing outbreaks in India are very much heterogeneous in the aa 130-160 region of VP1.
Sanyal
A
A
Central Laboratory, All India Coordinated Research Project on Foot-and-Mouth Disease, Indian Veterinary Research Institute, Nainital, India.
Gurumurthy
C B
CB
Venkataramanan
R
R
Hemadri
D
D
Tosh
C
C
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D005537:Aphthovirus / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D001208:Asia / Q000453:epidemiology; D002213:Capsid / Q000737:chemistry* / Q000235:genetics; D036022:Capsid Proteins; D002460:Cell Line; D016384:Consensus Sequence; D006224:Cricetinae; D004196:Disease Outbreaks; D005536:Foot-and-Mouth Disease / Q000453:epidemiology / Q000821:virology; D006801:Humans; D007194:India / Q000453:epidemiology; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry* / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012703:Serotyping
D000596:Amino Acids; D036022:Capsid Proteins; D010455:Peptides; C442501:VP1 protein, Foot-and-mouth disease virus