[Neuromuscular reinnervation of mimetic muscles. A histomorphologic study of the zygomatic muscle of the rabbit].
To evaluate occurrences of reinnervation and degeneration, the portio auricularis of the zygomatic muscle was reinnervated (either primary or secondary reinnervation) with an interval of 6 weeks, using a neuromuscular transplant of the sternohyoid muscle, which was left at the ansa hypoglossi. Histological examination was performed 6 and 12 weeks later. Enzymatic stains for myofibrillar ATPase were used for differentiation of muscle fibre types. Histomorphological features of denervation and reinnervation were analysed. Six weeks after denervation and primary neuromuscular transplantation, type grouping in regions close to the transplanted muscle were detectable as signs of reinnervation. After a period of 12 weeks, the progressing reinnervation was proved by type grouping in distal areas of the muscle far from the area of transplantation. At the same time, differentiation of fibre typing in the type grouping areas increased. All denervated and reinnervated muscles had pathological variations of fibre diameter. Primary reinnervated muscles showed tendency of normalization in variation of diameter. Compared with primary reinnervated muscles, secondary reinnervated muscles with an interval of 6 weeks showed pronounced polymorphology of fibres and a high pathological increase of the variation of fibre diameters. The interfascicular space was significantly spread. The tendency of reinnervation with incidence of fibre type grouping was comparable to primary reinnervated muscles and also occurred 6 weeks after transplantation.
Keiner
S
S
Klinik und Poliklinik für Hals-, Nasen-, Ohrenheilkunde und Plastische Operationen, Universität Leipzig.
Asmussen
G
G
Schulz
R
R
Bootz
F
F
D000818:Animals; D005152:Facial Muscles / Q000294:innervation*; D005158:Facial Paralysis / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D007002:Hypoglossal Nerve / Q000473:pathology / Q000503:physiopathology; D009121:Muscle Denervation; D009412:Nerve Fibers / Q000473:pathology / Q000502:physiology; D009416:Nerve Regeneration / Q000502:physiology*; D016067:Nerve Transfer / Q000379:methods*; D009469:Neuromuscular Junction / Q000473:pathology / Q000503:physiopathology; D011183:Postoperative Complications / Q000473:pathology / Q000503:physiopathology; D011817:Rabbits