Soybean genetic map of RAPD markers assigned to an existing scaffold RFLP map.
A 356-marker linkage map of Glycine max (L.) Merr. (2n = 20) was established by anchoring 106 RAPD markers to an existing RFLP map built with a large recombinant inbred line population (330 RILs). This map comprises 24 major and 11 minor linkage groups for this genome which is estimated to be approximately 3,275 cM. The RAPD markers show similar distribution throughout the genome and identified similar levels of polymorphism as the RFLP markers used in the framework. By using a subset population to anchor the RAPD markers, it was possible to enhance the throughput of selecting and adding reliable marker loci to the existing map. The procedures to generate a dependable genetic linkage map are also described in this report.
Ferreira
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AR
Rigesa Ltda, Dep Florestal, Canoinhas, Brazil. arnaldo.ferreira@rigesa.com.br
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Keim
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D002874:Chromosome Mapping / Q000379:methods*; D003433:Crosses, Genetic; D004247:DNA / Q000302:isolation & purification; D017931:DNA Primers / Q000737:chemistry; D005784:Gene Amplification; D005787:Gene Frequency; D017343:Genes, Plant; D008040:Genetic Linkage / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics*; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D013025:Glycine max / Q000235:genetics*
D017931:DNA Primers; D005819:Genetic Markers; D004247:DNA