Impaired cutaneous permeability barrier function, skin hydration, and sphingomyelinase activity in keratin 10 deficient mice.
Point mutations in the suprabasal cytokeratins 1 (K1) or 10 (K10) in humans have been shown to be the cause of the congenital ichthyosis epidermolytic hyperkeratosis. Recently, a K10 deficient mouse model was established serving as a model for epidermolytic hyperkeratosis. Homozygotes suffered from severe skin fragility and died shortly after birth. Heterozygotes developed hyperkeratosis with age. To see whether phenotypic abnormalities in the mouse model were associated with changes in skin barrier function and skin water content we studied basal transepidermal water loss and capacity for barrier repair after experimental barrier disruption as well as stratum corneum hydration. Also, we determined the activities of acid and neutral sphingomyelinase key enzymes of the tumor necrosis factor and interleukin-1 signal transduction pathways generating the ceramides most important for epidermal permeability barrier homeostasis. Neonatal homozygotes showed an 8-fold increase in basal transepidermal water loss compared with wild type controls. Adult heterozygotes exhibited delayed barrier repair after experimental barrier disruption. Stratum corneum hydration was reduced in homozygous and heterozygous mice. Acid sphingomyelinase activity, which is localized in the epidermal lamellar bodies and generates ceramides for extracellular lipid lamellae in the stratum corneum permeability barrier, was reduced in homozygous as well as heterozygous animals. Neutral sphingomyelinase activity, which has a different location and generates ceramides involved in cell signaling, was increased. The reduction in acid sphingomyelinase activity may explain the recently described decreased ratio of ceramides to total lipids in K10 deficient mice. In summary, our results demonstrate the crucial role of the keratin filament for permeability barrier function and stratum corneum hydration.
Jensen
J M
JM
Department of Dermatology and Institute of Immunology, University of Kiel, Germany.
Schütze
S
S
Neumann
C
C
Proksch
E
E
D000818:Animals; D000831:Animals, Newborn; D001834:Body Water / Q000378:metabolism*; D002463:Cell Membrane Permeability / Q000502:physiology*; D004195:Disease Models, Animal; D006579:Heterozygote; D006706:Homeostasis; D006720:Homozygote; D006801:Humans; D017488:Hyperkeratosis, Epidermolytic; D007633:Keratins / Q000172:deficiency*; D051379:Mice; D012867:Skin / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism; D013108:Sphingomyelin Phosphodiesterase / Q000378:metabolism*
D007633:Keratins; D013108:Sphingomyelin Phosphodiesterase