[Study on recombinant BCG to prevent infections of intracellular pathogens].
Studies on recombinant BCG (rBCG) which my group carried out so far were reviewed. Recombinant BCG which secreted alpha antigen-fused foreign antigen was constructed and tested for its ability to induce protective immunity. Thus, rBCG secreting merozoite surface protein 1 (MSP1) of Plasmodium yoelii efficiently protected the infection more than recombinant MSP 1 mixed with artificial adjuvant RAS or IFA did. rBCG which secreted excess amounts of antigen 85 complex A inhibited the multiplication of M. leprae in the footpads of mice. rBCG which secreted alpha antigen-fused IL-2 stimulated peritoneal exudate cells of mice resulting in enhancing killing a bladder cancer cell line in vitro.
Takeshi
Y
Y
Nagasaki University School of Dentistry, Japan.
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001500:BCG Vaccine; D020558:GTP Phosphohydrolases; D020066:Merozoite Surface Protein 1; D051379:Mice; D009163:Mycobacterium bovis / Q000276:immunology; D009166:Mycobacterium leprae / Q000254:growth & development; D011994:Recombinant Proteins; D014376:Tuberculosis / Q000517:prevention & control; D001749:Urinary Bladder Neoplasms / Q000628:therapy
D000942:Antigens, Bacterial; D001500:BCG Vaccine; D020066:Merozoite Surface Protein 1; D011994:Recombinant Proteins; D020558:GTP Phosphohydrolases