Evaluation of a human immunometric assay for the determination of thyroid-stimulating hormone in nonhuman primates.
Sera from nine species of clinically healthy nonhuman primates were assayed for T4 and thyroid-stimulating hormone (TSH) using human immunoassays (a fluorescence polarization immunoassay for T4, a microparticle enzyme immunoassay for TSH). The T4 levels ranged from 20 to 132 nmol/L (x +/- SD = 62.8 +/- 24.7 nmol/L). Levels of TSH were detected only in western lowland gorillas (Gorilla gorilla gorilla) and Sumatran orangutans (Pongo pygmaeus abelii) (range, 0.4-10.8 mIU/L; detectable limit = 0.01 mIU/L). The results suggest that the antibodies used in the commercial TSH immunoassay assessed in this study cross-react with gorilla and orang-utan TSH but not with TSH of primates of the genera Macaca, Papio, Erythrocebus, Ateles, Leontopithecus, and Lemur.
Lair
S
S
Toronto Zoo, Scarborough, Ontario, Canada.
Crawshaw
G J
GJ
Mehren
K G
KG
Perrone
M A
MA
D000818:Animals; D016231:Fluorescence Polarization Immunoassay / Q000662:veterinary*; D006071:Gorilla gorilla / Q000502:physiology; D000882:Haplorhini / Q000502:physiology; D006801:Humans; D007124:Immunoenzyme Techniques / Q000662:veterinary*; D008251:Macaca / Q000502:physiology; D010215:Papio / Q000502:physiology; D011148:Pongo pygmaeus / Q000502:physiology; D011323:Primates / Q000502:physiology*; D012452:Saguinus / Q000502:physiology; D013972:Thyrotropin / Q000032:analysis*; D013974:Thyroxine / Q000032:analysis*
D013972:Thyrotropin; D013974:Thyroxine