title: Acf7 (MACF) is an actin and microtubule linker protein whose expression predominates in neural, muscle, and lung development.
abstract: Several proteins belonging to the plakin family of cytoskeletal linker proteins have recently been identified, including dystonin/Bpag1 and plectin. These proteins are unique in their abilities to form bridges between different cytoskeletal elements through specialized modular domains. We have previously reported the cloning and partial characterization of Acf7, a novel member of the plakin family. More recently, the full-length cDNA for mouse Acf7 has been reported. Acf7 has a hybrid composition, with extended homology to dystonin/Bpag1 and plectin in the N-terminal half, and to dystrophin in the central and C-terminal half. Recent studies have demonstrated that Acf7 has functional actin and microtubule binding domains. Here, we describe the developmental expression profile for mouse Acf7. RNA in situ hybridization experiments revealed Acf7 transcripts in the dermomyotome and neural fold of day 8.5 mouse embryos. Later in development, Acf7 expression was predominant in neural and muscle tissues and was strongly up-regulated just before birth in type II alveolar cells of the lung. Altogether, our results suggest that Acf7 functions as a versatile cytoskeletal linker protein and plays an important role in neural, muscle, and lung development.
authors.affiliation: Centre for Molecular Medicine, Ottawa Hospital Research Institute, Ottawa, Ontario, Canada.
authors.forename: G
authors.initials: G
authors.lastname: Bernier
authors.forename: M
authors.initials: M
authors.lastname: Pool
authors.forename: M
authors.initials: M
authors.lastname: Kilcup
authors.forename: J
authors.initials: J
authors.lastname: Alfoldi
authors.forename: Y
authors.initials: Y
authors.lastname: De Repentigny
authors.forename: R
authors.initials: R
authors.lastname: Kothary
mesh_terms: D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000196:embryology / Q000254:growth & development / Q000502:physiology*; D005314:Embryonic and Fetal Development; D018507:Gene Expression Regulation, Developmental; D008168:Lung / Q000196:embryology / Q000254:growth & development / Q000502:physiology*; D051379:Mice; D008817:Mice, Mutant Strains; D008840:Microfilament Proteins / Q000235:genetics* / Q000378:metabolism; D008870:Microtubules / Q000502:physiology; D024510:Muscle Development; D009132:Muscles / Q000196:embryology / Q000502:physiology*; D009928:Organ Specificity; D012150:Polymorphism, Restriction Fragment Length; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013116:Spinal Cord / Q000196:embryology / Q000254:growth & development / Q000502:physiology*
chemical_list: C490632:Macf1 protein, mouse; D008840:Microfilament Proteins