title: In vitro analyses of diamond-like carbon coated stents. Reduction of metal ion release, platelet activation, and thrombogenicity.
abstract: Coronary artery stents can induce platelet activation by shear forces, contact to the biomaterial, and release of metal ions. This activation is one important trigger for thrombosis. Coating of stents is a possible approach to prevent this side effect. The purpose of this study was to evaluate in vitro the biocompatibility of stents coated with diamond-like carbon (DLC). Semiquantitative energy-dispersive X-ray microanalyses showed a complete coverage of the DLC stents. Flow cytometric analyses revealed a significantly higher increase of mean channel fluorescence intensity for the activation-dependent antigens CD62p and CD63 in non-coated compared to DLC-coated stents (p<0.05). Atomic adsorption spectrophotometry analyses revealed a significant release of nickel and chromium metal ions by non-coated stents over a storage period of 96 hours in human plasma (p<0.05). In contrast, only minimal concentrations of released ions could be detected in the case of DLC-coated stents. Similar observations were made with inductively coupled plasma mass spectrometry analyses. Here, high concentrations of molybdenum and manganese ions were released from non-coated stents (p<0.05), while release of these ions from DLC-coated stents was virtually undetectable (p=0.1 for molybdenum and p=0.4 for manganese). Coating of intracoronary stents with diamond-like carbon significantly improves biocompatibility. This biocompatible coating may therefore contribute to a reduction in thrombogenicity.
authors.affiliation: Departments of Transfusion Medicine and Transplantation Immunology, Hamburg, Germany. gutensohn@uke.uni-hamburg.de
authors.forename: K
authors.initials: K
authors.lastname: Gutensohn
authors.forename: C
authors.initials: C
authors.lastname: Beythien
authors.forename: J
authors.initials: J
authors.lastname: Bau
authors.forename: T
authors.initials: T
authors.lastname: Fenner
authors.forename: P
authors.initials: P
authors.lastname: Grewe
authors.forename: R
authors.initials: R
authors.lastname: Koester
authors.forename: K
authors.initials: K
authors.lastname: Padmanaban
authors.forename: P
authors.initials: P
authors.lastname: Kuehnl
mesh_terms: D000497:Alloys; D001792:Blood Platelets / Q000737:chemistry; D002244:Carbon; D002857:Chromium / Q000032:analysis; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000592:standards; D004577:Electron Probe Microanalysis; D005434:Flow Cytometry; D006801:Humans; D007477:Ions / Q000032:analysis; D013058:Mass Spectrometry; D019216:Metals, Heavy / Q000032:analysis; D009532:Nickel / Q000032:analysis; D015539:Platelet Activation; D011474:Prosthesis Design; D013054:Spectrophotometry, Atomic; D015607:Stents / Q000009:adverse effects / Q000592:standards*; D013927:Thrombosis / Q000209:etiology / Q000517:prevention & control; D013997:Time Factors
chemical_list: D000497:Alloys; D020099:Coated Materials, Biocompatible; D007477:Ions; D019216:Metals, Heavy; D002857:Chromium; D002244:Carbon; D009532:Nickel