[Cloning of taxadiene synthase cDNA from the cell line of Taxus cuspidata].
Taxadiene synthase plays an important role in taxol biosynthesis. RT-PCR was used for cloning taxadiene synthase cDNA fragment from the cells of T. cuspidata. The cDNA was cloned into vector pGEM and transformed to E. coli J M109. The cloned cDNA named pCBMZ was further confirmed by Southern blotting assay and was sequenced. The result showed that taxadiene synthase cDNA of Taxus cuspidata was highly homologous with that of Taxus brevifolia.
Hu
G W
GW
Nature Products Institute of Biochemistry, Tianjin University.
Ma
Z
Z
Wen
T Y
TY
Yuan
Y J
YJ
D001483:Base Sequence; D015139:Blotting, Southern; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification*; D007535:Isomerases / Q000235:genetics*; D008969:Molecular Sequence Data; D010946:Plants, Medicinal / Q000201:enzymology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018076:DNA, Complementary; D007535:Isomerases; C095926:taxa-4(5),11(12)-diene synthase