title: A novel PCR-based method for direct Fc gamma receptor IIIa (CD16) allotyping.
abstract: Leukocyte IgG receptors (Fc gamma R) are important immune-response modulating molecules. Fc gamma RIIIa is expressed on macrophages, NK-cells and gamma delta-T cells and exhibits a genetically determined, functional polymorphism at nucleotide 559. This allelic difference predicts either a phenylalanine (F158) or valine (V158) at amino acid 158 in the membrane-proximal extracellular domain, and has been shown to be associated with autoimmune and infectious diseases. Published methods to determine Fc gamma RIIIa genotypes are cumbersome. Therefore, we developed a novel, rapid and reliable PCR-based method to determine Fc gamma RIIIa genotypes. Comparison of genotyping results with direct Fc gamma RIIIa sequencing of 60 blood donors showed 100% accuracy of this new method. Since genotype frequencies of Fc gamma R polymorphisms depend strongly on race and ethnicity, we compared Fc gamma RIIIa genotype frequencies of 176 Caucasian Dutch and 104 Japanese blood donors. Interestingly, these frequencies were not significantly different (P>0.1), in contrast to the Fc gamma RIIa and Fc gamma RIIIb genotype frequencies (P<0.001).
authors.affiliation: Immunotherapy Laboratory, Department of Immunology, KC02.085-2, University Medical Center Utrecht, Lundlaan 6, 3584 EA Utrecht, The Netherlands.
authors.forename: F G
authors.initials: FG
authors.lastname: Leppers-van de Straat
authors.forename: W L
authors.initials: WL
authors.lastname: van der Pol
authors.forename: M D
authors.initials: MD
authors.lastname: Jansen
authors.forename: N
authors.initials: N
authors.lastname: Sugita
authors.forename: H
authors.initials: H
authors.lastname: Yoshie
authors.forename: T
authors.initials: T
authors.lastname: Kobayashi
authors.forename: J G
authors.initials: JG
authors.lastname: van de Winkel
mesh_terms: D000483:Alleles; D044466:Asian People / Q000235:genetics; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007126:Immunoglobulin Allotypes; D007564:Japan; D009426:Netherlands; D016133:Polymerase Chain Reaction / Q000379:methods*; D017452:Receptors, IgG / Q000145:classification / Q000235:genetics*; D015203:Reproducibility of Results; D044465:White People / Q000235:genetics
chemical_list: D007126:Immunoglobulin Allotypes; D017452:Receptors, IgG