title: Antiestrogenicity of beta-naphthoflavone and PAHs in cultured rainbow trout hepatocytes: evidence for a role of the arylhydrocarbon receptor.
abstract: The aims of the present study were to assess, (1) if polyaromatic hydrocarbons (PAHs) are able to inhibit estradiol-regulated vitellogenin synthesis in fish; and (2) if this antiestrogenic activity is mediated through the binding of PAHs to the arylhydrocarbon receptor (AhR). Cultured liver cells of rainbow trout, Oncorhynchus mykiss, were co-exposed to PAHs and 17beta-estradiol (E2), and the resulting effects on induction of AhR-regulated 7-ethoxyresorufin-O-deethylase (EROD) activity and on E2-regulated vitellogenesis were investigated. The following test compounds were compared: the PAH 3-methylcholanthrene (3MC), which is a strong EROD inducer, the PAH anthracene (ANT), which is not an inducer of EROD activity, and the model EROD inducer, beta-naphthoflavone (betaNF). 3MC and betaNF led to significant decreases of E2-triggered hepatocellular VTG synthesis, whereas ANT exerted no antiestrogenic activity. The rank order of the antiestrogenic activity of the test substances agreed with their EROD-inducing potency suggesting that their antiestrogenicity might be mediated through the AhR. Further evidence for this assumption comes from the observation that inhibitors such as alpha-naphthoflavone which interferes with ligand-AhR binding, and 8-methoxypsoralen (8MP), which prevents binding of the occupied AhR to responsive DNA elements, clearly reduced the antiestrogenic effects of the xenobiotics. Furthermore, from the comparison of estradiol concentrations in media of liver cells exposed to the CYP 1A-inducing agents and in media of control cells it is unlikely that the observed antiestrogenic effects were caused by an enhanced E2 catabolism. In conclusion, the results from this study indicate that, (1) AhR-binding PAHs possess an antiestrogenic activity; and (2) that the antiestrogenic activity is mediated through the AhR.
authors.affiliation: Umweltforschungszentrum Leipzig-Halle, Sektion Chemische Okotoxikologie, Permoserstrasse 15, D-04318, Leipzig, Germany.
authors.forename: J M
authors.initials: JM
authors.lastname: Navas
authors.forename: H
authors.initials: H
authors.lastname: Segner
mesh_terms: D000818:Animals; D000873:Anthracenes / Q000633:toxicity; D002273:Carcinogens / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D019363:Cytochrome P-450 CYP1A1 / Q000096:biosynthesis; D004797:Enzyme-Linked Immunosorbent Assay; D004965:Estrogen Antagonists / Q000633:toxicity*; D005260:Female; D022781:Hepatocytes / Q000187:drug effects*; D008297:Male; D008730:Methoxsalen / Q000633:toxicity; D008748:Methylcholanthrene / Q000633:toxicity; D017686:Oncorhynchus mykiss / Q000502:physiology*; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity*; D014176:Protein Biosynthesis; D018336:Receptors, Aryl Hydrocarbon / Q000187:drug effects*; D014819:Vitellogenins / Q000096:biosynthesis; D019324:beta-Naphthoflavone / Q000633:toxicity*
chemical_list: D000873:Anthracenes; D002273:Carcinogens; D004965:Estrogen Antagonists; D011084:Polycyclic Aromatic Hydrocarbons; D018336:Receptors, Aryl Hydrocarbon; D014819:Vitellogenins; D008748:Methylcholanthrene; D019324:beta-Naphthoflavone; D019363:Cytochrome P-450 CYP1A1; D008730:Methoxsalen