title: 5'-Methoxyhydnocarpin-D and pheophorbide A: Berberis species components that potentiate berberine growth inhibition of resistant Staphylococcus aureus.
abstract: A new method of bioactivity-directed fractionation, based on multidrug resistant pump (MDR) inhibition in Staphylococcus aureus, was demonstrated. This resulted in the isolation, from berberine-containing Berberis species, of two compounds that are themselves devoid of S. aureus antibacterial activity, but that form potent synergistic couples with a subinhibitory concentration of berberine. The bacterial MDR pump inhibitors were identified as the flavonolignan 2 and the porphyrin 3. Another natural flavonolignan, silybin (8) from Silybum marianum, was also shown to be a bacterial MDR pump inhibitor.
authors.affiliation: Department of Chemistry, Colorado State University, Fort Collins, Colorado 80523-1872, USA. frslab@lamar.colostate.edu
authors.forename: F R
authors.initials: FR
authors.lastname: Stermitz
authors.forename: J
authors.initials: J
authors.lastname: Tawara-Matsuda
authors.forename: P
authors.initials: P
authors.lastname: Lorenz
authors.forename: P
authors.initials: P
authors.lastname: Mueller
authors.forename: L
authors.initials: L
authors.lastname: Zenewicz
authors.forename: K
authors.initials: K
authors.lastname: Lewis
mesh_terms: D001599:Berberine / Q000494:pharmacology*; D001681:Biological Assay; D002734:Chlorophyll / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D003120:Colorado; D004352:Drug Resistance, Microbial; D018432:Drug Resistance, Multiple; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013056:Spectrophotometry, Ultraviolet; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development
chemical_list: C405502:5'-methoxyhydnocarpin; D005419:Flavonoids; D001599:Berberine; D002734:Chlorophyll; C032623:pheophorbide a