Oral immunogenicity of the plant derived spike protein from swine-transmissible gastroenteritis coronavirus.
Transgenic plants represent an inexpensive alternative to classical fermentation systems for production of recombinant subunit vaccines. Transgenic potato plants were created that express the N-terminal domain of the glycoprotein S (N-gS) from Transmissible gastroenteritis coronavirus (TGEV), containing the major antigenic sites of the protein. Extracts from potato tubers expressing N-gS were inoculated intraperitoneally to mice, and the vaccinated mice developed serum IgG specific for TGEV. Furthermore, when potato tubers expressing N-gS were fed directly to mice, they developed serum antibodies specific for gS protein, demonstrating the oral immunogenicity of the plant derived spike protein from TGEV.
Gómez
N
N
Departamento de Mejora Genética y Biotecnologí INIA, Madrid, Spain.
Wigdorovitz
A
A
Castañón
S
S
Gil
F
F
Ordás
R
R
Borca
M V
MV
Escribano
J M
JM
D000284:Administration, Oral; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000008:administration & dosage; D017934:Coronavirus / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D018352:Coronavirus Infections / Q000517:prevention & control; D004797:Enzyme-Linked Immunosorbent Assay; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010940:Plant Proteins / Q000235:genetics; D030821:Plants, Genetically Modified; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000008:administration & dosage; D011198:Solanum tuberosum; D064370:Spike Glycoprotein, Coronavirus; D014170:Transformation, Genetic; D014614:Vaccines, Synthetic / Q000008:administration & dosage; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage
D000914:Antibodies, Viral; D000956:Antigens, Viral; D008562:Membrane Glycoproteins; D010940:Plant Proteins; D011994:Recombinant Proteins; D064370:Spike Glycoprotein, Coronavirus; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D014765:Viral Vaccines