Expression and immunolocalization of multidrug resistance protein 2 in rabbit small intestine.
Multidrug resistance protein 2 (MRP2) is an ATP-dependent transporter of anionic drugs and conjugates. It functions as an efflux pump in the apical membranes of liver and kidney cells, but its membrane localization in small intestine has not yet been defined. The present study demonstrates exclusive localization of Mrp2 to the brush-border (apical) membrane of villi, decreasing in intensity from the villus tip to the crypts. In immunoblot analysis of crude membranes of various rabbit tissues, Mrp2 was only found in small intestine, kidney and liver. These results are in-line with the supposed function of Mrp2 in drug excretion.
Van Aubel
R A
RA
Department of Pharmacology and Toxicology, UMC St. Radboud, University of Nijmegen, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands.
Hartog
A
A
Bindels
R J
RJ
Van Os
C H
CH
Russel
F G
FG
D000818:Animals; D027321:Anion Transport Proteins; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000276:immunology; D004386:Duodenum / Q000378:metabolism / Q000648:ultrastructure; D019084:Fluorescent Antibody Technique, Indirect; D006168:Guinea Pigs; D015151:Immunoblotting; D007421:Intestine, Small / Q000378:metabolism* / Q000648:ultrastructure; D008871:Microvilli / Q000378:metabolism; D011817:Rabbits; D051381:Rats
D027321:Anion Transport Proteins; D000906:Antibodies; D002352:Carrier Proteins