Electron microscopic examination of subcellular fractions. I. The preparation of representative samples from suspensions of particles.
A method is described for preparing, by filtration on Millipore filters, very thin (about 10 micro) pellicles of packed particles. These pellicles can be embedded in Epon for electron microscopic examination. They are also suitable for cytochemical assays. The method was used with various particulate fractions from rat liver. Its main advantages over the usual centrifugal packing techniques are that it produces heterogeneity solely in the direction perpendicular to the surface of the pellicle and that sections covering the whole depth of the pellicle can be photographed in a single field. It thus answers the essential criterion of random sampling and can be used for accurate quantitative evaluations.
Baudhuin
P
P
Evrard
P
P
Berthet
J
J
D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D004853:Epoxy Resins; D005260:Female; D005374:Filtration; D008099:Liver / Q000737:chemistry / Q000201:enzymology; D008854:Microscopy, Electron / Q000379:methods*; D008861:Microsomes / Q000737:chemistry / Q000201:enzymology / Q000648:ultrastructure; D010316:Particle Size; D051381:Rats; D017208:Rats, Wistar; D013347:Subcellular Fractions / Q000737:chemistry / Q000201:enzymology / Q000648:ultrastructure*; D016610:Tissue Embedding / Q000379:methods
D004853:Epoxy Resins; C004875:EPON; D000135:Acid Phosphatase