Rapid detection of Staphylococcus aureus using bioluminescent enzyme immunoassay.
A bioluminescent enzyme immunoassay (BLEIA) method for detecting protein A-bearing Staphylococcus aureus was developed using biotinylated firefly luciferase. The BLEIA was able to detect protein A at one pg ml-1 and 103 cfu ml-1 level of Staph. aureus. The BLEIA showed significant signals with overnight cultures of all 24 Staph. aureus strains, and the BLEIA did not show any significant signals with overnight cultures of all 44 strains of coagulase-negative staphylococci and the other genus bacteria. After 5 h cultivation beginning at approximately 50 cfu ml-1, the BLEIA was able to detect all 35 Staph. aureus strains isolated from healthy humans.
Fukuda
S
S
Research and Development Division, Kikkoman Corporation, Chiba, Japan. s.fukuda@mail-kikkoman.co.jp
Tatsumi
H
H
Igarashi
H
H
Igimi
S
S
D001426:Bacterial Proteins / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D008156:Luciferases / Q000378:metabolism; D008163:Luminescent Measurements; D013205:Staphylococcal Protein A / Q000378:metabolism*; D013210:Staphylococcus / Q000145:classification / Q000302:isolation & purification; D013211:Staphylococcus aureus / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism
D001426:Bacterial Proteins; D013205:Staphylococcal Protein A; D008156:Luciferases