Serotonergic retinopetal projections from the dorsal raphe nucleus in the mouse demonstrated by combined [(3)H] 5-HT retrograde tracing and immunolabeling of endogenous 5-HT.
The present study demonstrated a direct serotonergic retinopetal projection in the mouse stemming from the lateral portion of the dorsal raphe nucleus bilaterally. A double-labeling technique was employed combining: (1) radioautography and retrograde axonal tracing following intraocular injection of [(3)H] 5-HT and (2) immunocytochemical identification of endogenous 5-HT. Radiolabeled neurons were only observed within the dorsal raphe nucleus and were always double-labeled with the 5-HT antibody. The radiolabeling appeared to be specific resulting from the retrograde transport of a radioactive 5-HT derivative product following uptake of the neurotransmitter by intraretinal terminals.
Repérant
J
J
INSERM U 106, Laboratoire de Neuromorphologie, Développement, Evolution, Batiment de Pédiatrie, Hôpital de la Salpêtrière, Paris, France.
Araneda
S
S
Miceli
D
D
Medina
M
M
Rio
J P
JP
D000818:Animals; D001345:Autoradiography; D001370:Axonal Transport; D001693:Biological Transport, Active; D004525:Efferent Pathways / Q000502:physiology; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D011903:Raphe Nuclei / Q000502:physiology*; D012160:Retina / Q000502:physiology*; D012701:Serotonin / Q000378:metabolism / Q000502:physiology*; D009435:Synaptic Transmission / Q000502:physiology*
D012701:Serotonin