title: Reduction of aflatoxins by Korean soybean paste and its effect on cytotoxicity and reproductive toxicity--part 1. Inhibition of growth and aflatoxin production of Aspergillus parasiticus by Korean soybean paste (Doen-jang) and identification of the active component.
abstract: The inhibitory effect of methanol extract of Korean soybean paste on the mold growth and aflatoxin production of a toxigenic strain of Aspergillus parasiticus ATCC 15517 was studied using different concentrations of the extract in yeast-extract sucrose broth. While inhibition in mold growth due to increasing the concentration of the extract was observed, the more remarkable effect was the inhibition of aflatoxin production. Reduction of mycelial weight as a result of addition of the extract was observed to range between 1.5 to 12.9% while reduction of aflatoxin production quantified by high-performance liquid chromatography ranged from 14.3 to 41.7%. Five percent of the extract significantly reduced aflatoxin production at the end of the incubation period (P < 0.05), although the effect on mycelial growth was less pronounced. This study indicates that soybean paste could also be an effective inhibitor of aflatoxin production even though mycelial growth may be permitted. The main active component identified by gas chromatography-mass spectroscopy was linoleic acid.
authors.affiliation: Department of Public Health, Keimyung University, Taegu, Korea. jgkim@kmucc.kmu.ac.kr
authors.forename: J G
authors.initials: JG
authors.lastname: Kim
authors.forename: Y W
authors.initials: YW
authors.lastname: Lee
authors.forename: P G
authors.initials: PG
authors.lastname: Kim
authors.forename: W S
authors.initials: WS
authors.lastname: Roh
authors.forename: H
authors.initials: H
authors.lastname: Shintani
mesh_terms: D000348:Aflatoxins / Q000096:biosynthesis*; D001230:Aspergillus / Q000254:growth & development / Q000378:metabolism* / Q000472:pathogenicity; D002846:Chromatography, Affinity; D003603:Cytotoxins / Q000009:adverse effects*; D007723:Korea; D019787:Linoleic Acid; D000432:Methanol; D009824:Ointments; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D013025:Glycine max / Q000737:chemistry / Q000502:physiology*
chemical_list: D000348:Aflatoxins; D003603:Cytotoxins; D009824:Ointments; D010936:Plant Extracts; D019787:Linoleic Acid; D000432:Methanol