title: Hyaluronic acid/carboxymethylcellulose membrane surrounding an intraperitoneal or subcutaneous jejunojejunostomy in rats.
abstract: OBJECTIVE
  To investigate the healing of intra-abdominal and extra-abdominal jejunal anastomoses that were surrounded by a hyaluronic acid/carboxymethylcellulose membrane (Seprafilm) in rats.
  
  
  METHODS
  Laboratory study.
  
  
  METHODS
  University hospital, The Netherlands.
  
  
  METHODS
  56 male Wistar rats.
  
  
  METHODS
  28 rats had jejunojejunostomies placed subcutaneously and 28 had them placed intra-abdominally. Half of each group of anastomoses were surrounded by Seprafilm.
  
  
  METHODS
  After 3 or 7 days, the anastomoses were tested for bursting pressure, tensile strength, and hydroxyproline concentrations.
  
  
  RESULTS
  There were numerous strong adhesions around the anastomoses in the subcutaneous position and Seprafilm had no influence on their extent. Bursting pressure and hydroxyproline concentrations were not affected by position or Seprafilm treatment. Tensile strength was significantly higher in the subcutaneous position (p < 0.01), but was unaffected by Seprafilm.
  
  
  CONCLUSIONS
  Anastomotic healing was not impaired in the subcutaneous or intraperitoneal position in this model. Seprafilm had no effect on the anastomosis.
authors.affiliation: Department of Surgery, Academic Medical Center at the University of Amsterdam, The Netherlands.
authors.forename: F J
authors.initials: FJ
authors.lastname: van Oosterom
authors.forename: J J
authors.initials: JJ
authors.lastname: van Lanschot
authors.forename: J
authors.initials: J
authors.lastname: Oosting
authors.forename: H
authors.initials: H
authors.lastname: Obertop
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D002266:Carboxymethylcellulose Sodium; D006820:Hyaluronic Acid; D007582:Jejunostomy / Q000379:methods; D007583:Jejunum / Q000601:surgery; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012086:Reoperation; D014945:Wound Healing / Q000502:physiology*
chemical_list: D006820:Hyaluronic Acid; D002266:Carboxymethylcellulose Sodium