Isolation and partial characterization of cDNA clone of human ceruloplasmin receptor.
An individual clone, presumably carrying a 3 bp fragment of ceruloplasmin receptor cDNA was isolated from the expression library of human placenta cDNA using polyclonal specific antibodies to ceruloplasmin receptors. EcoR1-hydrolysate of isolated DNA was cloned in a pTZ19 bacterial vector and sequenced in the forward and reverse direction. The comparison of the revealed sequence with known sequences of human genome revealed its high similarity to ceruloplasmin cDNA.
Sasina
L K
LK
Department of Molecular Genetics, Institute of Experimental Medicine, Russian Academy of Medical Sciences, St. Petersburg. ludmila@urs8.iem.ras.spb.ru
Tsymbalenko
N V
NV
Platonova
N A
NA
Puchkova
L V
LV
Voronina
O V
OV
Gyulikhandanova
N E
NE
Gaitskhoki
V S
VS
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D015894:Genome, Human; D006801:Humans; D008969:Molecular Sequence Data; D011971:Receptors, Immunologic / Q000235:genetics*; D018000:Receptors, Peptide / Q000235:genetics*
D018076:DNA, Complementary; D011971:Receptors, Immunologic; D018000:Receptors, Peptide; C040519:ceruloplasmin receptor