Analysis of the domain requirement in Gas1 growth suppressing activity.
The product of the growth arrest specific gene, gas1, is a membrane-associated protein which activates a p53-dependent growth suppression signalling pathway. We have shown that Gas1 is linked to the plasma membrane through a glycosyl-phosphatidylinositol (GPI) anchor. Several GPI-anchored protein have been identified as part of receptor complexes either as co-receptors or as membrane bound ligands. In this report, we characterize the Gas1 domains required for its growth suppression function and demonstrate the dispensability of Gas1 GPI anchor.
Ruaro
M E
ME
Laboratorio Nazionale Consorzio Interuniversitario per le Bitecnologie, Area Science Park, Padriciano 99, 34012 Trieste, Italy.
Stebel
M
M
Vatta
P
P
Marzinotto
S
S
Schneider
C
C
D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D019556:COS Cells; D002455:Cell Division; D017261:Glycosylphosphatidylinositols / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008562:Membrane Glycoproteins / Q000008:administration & dosage / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008845:Microinjections; D009154:Mutation; D021382:Protein Sorting Signals / Q000235:genetics / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D017384:Sequence Deletion / Q000235:genetics; D012995:Solubility; D014162:Transfection
C066709:GAS1 protein, S cerevisiae; D017261:Glycosylphosphatidylinositols; D008562:Membrane Glycoproteins; D021382:Protein Sorting Signals; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D000469:Alkaline Phosphatase