title: Calcification processes in the chick otoconia and calcium binding proteins: patterns of tetracycline incorporation and calbindin-D28K distribution.
abstract: In order to clarify the otoconia formation and turnover, tetracycline, an antibiotic that precipitates at calcifying fronts and serves as a fluorescent marker, was injected into eggs at different stages of chick embryonic development, as well as into postnatal chicken and into adult animals. The changes in the intensity, location patterns and time course of fluorescent labelling in each examined stage in the otolithic organs was studied. The presence and distribution of calbindin (CB)-D28K, one of the calcium-binding proteins constantly found in the mammalian and chicken cochlea and also in otolithic membrane of some adult mammals, was studied. Results in embryonal stages, postnatal and adult animals allow us to postulate that otoliths are mainly produced during the embryonal phase, but they may also be produced throughout the whole life span. Results also indicate that otoconia are dynamic structures which undergo turnover. The correspondence between the patterns of CB-D28K immunoreactivity and tetracycline fluorescence may indicate that CB-D28K participates in the formation of otoconia.
authors.affiliation: Department of Genetics, General and Molecular Biology, University of Naples Federico II, via Mezzocannone 8, 80134 Naples, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Balsamo
authors.forename: B
authors.initials: B
authors.lastname: Avallone
authors.forename: F
authors.initials: F
authors.lastname: Del Genio
authors.forename: S
authors.initials: S
authors.lastname: Trapani
authors.forename: F
authors.initials: F
authors.lastname: Marmo
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development / Q000502:physiology; D064026:Calbindins; D002113:Calcification, Physiologic / Q000502:physiology*; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002642:Chick Embryo; D010037:Otolithic Membrane / Q000502:physiology*; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D013752:Tetracycline / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D064026:Calbindins; D002135:Calcium-Binding Proteins; D064030:S100 Calcium Binding Protein G; D013752:Tetracycline