title: Spatial distribution of 16S rRNA levels from uncultured acidobacteria in soil.
abstract: The activity of uncultured acidobacteria was monitored in Dutch grassland soils by quantifying their ribosomes. These bacteria were detectable by five different 16S rRNA RT-PCR products in temperature gradient gel electrophoresis fingerprints. The ribosomes in surface soil samples were quantified with multiple competitive RT-PCR along a 1.5-km transect through the grassland. In total, the five members of the acidobacteria were estimated to contribute 4 x 1010 to 1 x 1011 ribosomes g soil-1, representing 7-14% of all bacterial ribosomes. These results indicate that ribosomes from acidobacteria are continuously present and abundant in soil and might contribute significantly to microbial activity in soil.
authors.affiliation: Laboratory of Microbiology, Wageningen University, The Netherlands. afe@gbf.de
authors.forename: A
authors.initials: A
authors.lastname: Felske
authors.forename: W M
authors.initials: WM
authors.lastname: de Vos
authors.forename: A D
authors.initials: AD
authors.lastname: Akkermans
mesh_terms: D001419:Bacteria / Q000235:genetics* / Q000254:growth & development; D017753:Ecosystem; D006863:Hydrogen-Ion Concentration; D006109:Poaceae; D012329:RNA, Bacterial / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012988:Soil Microbiology
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S