Cloning of the murine thymic stromal lymphopoietin (TSLP) receptor: Formation of a functional heteromeric complex requires interleukin 7 receptor.
The cellular receptor for murine thymic stromal lymphopoietin (TSLP) was detected in a variety of murine, but not human myelomonocytic cell lines by radioligand binding. cDNA clones encoding the receptor were isolated from a murine T helper cell cDNA library. TSLP receptor (TSLPR) is a member of the hematopoietin receptor family. Transfection of TSLPR cDNA resulted in only low affinity binding. Cotransfection of the interleukin 7 (IL-7)Ralpha chain cDNA resulted in conversion to high affinity binding. TSLP did not activate cells from IL-7Ralpha(-/)- mice, but did activate cells from gammac(-/)- mice. Thus, the functional TSLPR requires the IL-7Ralpha chain, but not the gammac chain for signaling.
Park
L S
LS
Department of Biochemistry, Immunex Corporation, Seattle, Washington 98101, USA.
Martin
U
U
Garka
K
K
Gliniak
B
B
Di Santo
J P
JP
Muller
W
W
Largaespada
D A
DA
Copeland
N G
NG
Jenkins
N A
NA
Farr
A G
AG
Ziegler
S F
SF
Morrissey
P J
PJ
Paxton
R
R
Sims
J E
JE
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D016207:Cytokines / Q000494:pharmacology; D006410:Hematopoiesis / Q000187:drug effects*; D006801:Humans; D015851:Interleukin-7 / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects* / Q000502:physiology; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D018121:Receptors, Cytokine / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D020395:Receptors, Interleukin-7 / Q000737:chemistry / Q000502:physiology*; D011994:Recombinant Proteins / Q000737:chemistry; D015398:Signal Transduction
D016207:Cytokines; D015851:Interleukin-7; D012333:RNA, Messenger; D018121:Receptors, Cytokine; D020395:Receptors, Interleukin-7; D011994:Recombinant Proteins