Nerve growth factor and retinoic acid inhibit proliferation and invasion in thyroid tumor cells.
NGF has anti-proliferative and anti-invasive effects in neuroendocrine tumors. In the present work we examined the effects of NGF and retinoic acid on cell proliferation and invasion in thyroid carcinoma cells. We found that NGF and retinoic acid do not affect cell proliferation on their own but in combination they produce a strong inhibition. We also found that retinoic acid regulates the matrix metalloproteinase 2 activity and invasion. In contrast, NGF inhibited invasion and reverted the effect of retinoic acid. This effect of NGF is likely mediated by an increase in adhesion to laminin and collagen IV and the inhibition of cell migration. NGF also induced the expression of the p75 NGF receptor. In conclusion, NGF and retinoic acid in combination inhibit proliferation and invasion of thyroid papillary carcinoma cells. These data open the possibility of a potential combined therapy for thyroid papillary carcinomas.
Páez Pereda
M
M
Max Planck Institute of Psychiatry, Department of Endocrinology, Kraepelinstr. 10, 80804, Munich, Germany. marcelo@mpipsykl.mpg.de
Missale
C
C
Grübler
Y
Y
Arzt
E
E
Schaaf
L
L
Stalla
G K
GK
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002291:Carcinoma, Papillary / Q000378:metabolism / Q000473:pathology*; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D009361:Neoplasm Invasiveness; D020932:Nerve Growth Factor / Q000494:pharmacology*; D020800:Receptor, Nerve Growth Factor / Q000235:genetics / Q000378:metabolism; D013964:Thyroid Neoplasms / Q000378:metabolism / Q000473:pathology*; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D020800:Receptor, Nerve Growth Factor; D014212:Tretinoin; D020932:Nerve Growth Factor; D020778:Matrix Metalloproteinase 2