Capillary electrophoresis-inductively coupled plasma mass spectrometry interface with minimised dead volume for high separation efficiency.
A modified microconcentric nebulizer (MCN-100) has been used to construct an improved interface with minimised liquid and gas phase dead volumes for the coupling of capillary electrophoresis (CE) and inductively coupled plasma mass spectrometry (ICP-MS). A plate number of 3.6 x 10(6) plates m(-1) has been achieved. This is an order of magnitude better than the results previously reported for CE-ICP-MS. The separation efficiency of the system is demonstrated by the baseline separation of eight rare earth metals within a time span of 14.6 s. The system was used to control the purity of vitamin B12 and glutaredoxin 2 from Escherichia coli.
Tangen
A
A
University of Oslo, Department of Chemistry, Norway. anders.tangen@eu.apbiotech.com
Lund
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W
D019075:Electrophoresis, Capillary / Q000379:methods*; D004926:Escherichia coli / Q000737:chemistry; D054477:Glutaredoxins; D013058:Mass Spectrometry / Q000379:methods*; D010088:Oxidoreductases; D011506:Proteins / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D014805:Vitamin B 12 / Q000302:isolation & purification
D054477:Glutaredoxins; D011506:Proteins; D010088:Oxidoreductases; D014805:Vitamin B 12