Influence of autonomic neurotransmitters on human uveal melanocytes in vitro.
The influence of autonomic neurotransmitters on the growth and melanogenesis of cultured uveal melanocytes was studied. Uveal melanocytes were cultured with medium supplemented with cAMP elevating agents and basic fibroblast growth factor (complete medium). The cells were plated into multiple well plates, and various concentrations of adrenergic and cholinergic agents were added to the media (complete medium or various deleted media). After 6 days, the cells were detached for cell counting and melanin measurement and compared to controls. Epinephrine, isoproterenol, salbutamol and metaproterenol (adrenergic agonists that can activate beta(2)-adrenoceptors) substantially stimulated growth and melanogenesis of cultured uveal melanocytes in cAMP-deleted medium. Methoxamine, clonidine, prenalterol and D-7114 (adrenergic agonists that do not activate beta(2)-adrenoceptors) showed no effect under similar experimental conditions. Muscarine (a cholinergic agonist) inhibited the growth and melanogenesis of uveal melanocytes in complete medium. It indicates that adrenergic agents (beta(2)-adrenoceptor agonists) stimulate growth and melanogenesis in uveal melanocytes, while cholinergic agonist has an inhibitory effect. This effect appears to involve the cAMP second messenger system. These studies suggest that homeostasis of the uveal melanocytes may be maintained, in part, by regulating the autonomic nervous system in vivo.
Hu
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Tissue Culture Center, Departments of Pathology, Laboratory Medicine and Ophthalmology, The New York Eye and Ear Infirmary, 310 E. 14th Street, New York, NY 10003, USA.
Woodward
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McCormick
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D000322:Adrenergic Agonists / Q000494:pharmacology; D001341:Autonomic Nervous System / Q000502:physiology*; D002452:Cell Count; D002478:Cells, Cultured; D018679:Cholinergic Agonists / Q000494:pharmacology; D000242:Cyclic AMP / Q000502:physiology; D016222:Fibroblast Growth Factor 2 / Q000502:physiology; D006732:Horner Syndrome / Q000150:complications; D006801:Humans; D008543:Melanins / Q000502:physiology; D008544:Melanocytes / Q000187:drug effects / Q000502:physiology*; D018377:Neurotransmitter Agents / Q000502:physiology*; D010859:Pigmentation Disorders / Q000209:etiology / Q000503:physiopathology; D014602:Uvea / Q000166:cytology*
D000322:Adrenergic Agonists; D018679:Cholinergic Agonists; D008543:Melanins; D018377:Neurotransmitter Agents; D016222:Fibroblast Growth Factor 2; D000242:Cyclic AMP