title: hupS and hupL constitute a transcription unit in the cyanobacterium Nostoc sp. PCC 73102.
abstract: The heterocystous cyanobacterium Nostoc sp. PCC 73102 possesses an uptake hydrogenase encoded by hupS and hupL. The genes are transcribed in cells grown under N2-fixing but not under non-N2-fixing conditions. We characterised the transcription of hupS and hupL in further detail. A transcription start site was located at a position 259 bp upstream from the hupS translation start. As shown by RT-PCR, hupS and hupL constitute a single transcript in Nostoc sp. PCC 73102. The possible function of short, tandemly repeated repetitive sequences between hupS and hupL, enabling a folding of the entire intergenic region into a hairpin, is discussed.
authors.affiliation: Department of Physiological Botany, EBC, Uppsala University, Sweden. pia.lindberg@ebc.uu.se
authors.forename: P
authors.initials: P
authors.lastname: Lindberg
authors.forename: A
authors.initials: A
authors.lastname: Hansel
authors.forename: P
authors.initials: P
authors.lastname: Lindblad
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D000458:Cyanobacteria / Q000201:enzymology / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009586:Nitrogen Fixation; D009690:Nucleic Acid Conformation; D010088:Oxidoreductases / Q000235:genetics; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics; D020080:Tandem Repeat Sequences; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D010088:Oxidoreductases; C029746:uptake hydrogenase; C091789:HupL protein, Anabaena