Measurement of nitric oxide released in the rat heart with an amperometric microsensor.
Nitric oxide (NO) plays an important role in various physiological processes, acting either as an intra- and intercellular messenger or as a toxic agent. The detection and quantification of NO have been accomplished by a variety of methodologies. In the present study, real-time production of NO in the rat heart was continuously measured by using a novel copper-platinum microparticle-modified NO electrochemical microsensor. The linearity range of the microsensor is between 8.0 x 10(-8) and 4.8 x 10(-6) mol L-1 and the detection limit is 3.0 x 10(-8) mol L-1. NO release from the rat heart stimulated by the agonists L-arginine and acetylcholine was observed, and the responses were decreased by the NO synthase inhibitor L-N omega-nitroarginine. In addition, the effect of sodium nitroprusside (SNP), a NO donor, was also studied. SNP increases the concentration of NO in the rat heart. The experiments showed that electrochemical detection is suitable for detecting and quantifying NO in biological systems.
Xian
Y
Y
Department of Chemistry, East China Normal University, Shanghai, China.
Zhang
W
W
Xue
J
J
Ying
X
X
Jin
L
L
Jin
J
J
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001120:Arginine / Q000494:pharmacology; D004563:Electrochemistry; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008297:Male; D009206:Myocardium / Q000737:chemistry*; D009569:Nitric Oxide / Q000032:analysis*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D019335:Nitroarginine / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity; D014665:Vasodilator Agents / Q000494:pharmacology
D004791:Enzyme Inhibitors; D014665:Vasodilator Agents; D019335:Nitroarginine; D009569:Nitric Oxide; D001120:Arginine; D019001:Nitric Oxide Synthase; D000109:Acetylcholine