title: Purification and characterization of a surface-binding protein from Lactobacillus fermentum RC-14 that inhibits adhesion of Enterococcus faecalis 1131.
abstract: Lactobacilli have been shown to be important in the maintenance of the healthy urogenital flora. One strain, Lactobacillus fermentum RC-14, releases surface-active components which can inhibit adhesion of uropathogenic bacteria. Using a quantitative method for determining inhibition of adhesion, a protein with high anti-adhesive properties against Enterococcus faecalis 1131 was purified. The N-terminal sequence of the 29-kDa protein was identical to that of a collagen-binding protein from Lactobacillus reuteri NCIB 11951, and exhibited close homology with a basic surface protein from L. fermentum BR11. The results suggest that this anti-adhesive cell surface protein of Lactobacillus could protect against uropathogens by preventing their adhesion. the Federation of European Microbiological Societies.
authors.affiliation: Department of Microbiology and Immunology, University of Western Ontario Health Sciences Centre, London, Ont., Canada.
authors.forename: C
authors.initials: C
authors.lastname: Heinemann
authors.forename: J E
authors.initials: JE
authors.lastname: van Hylckama Vlieg
authors.forename: D B
authors.initials: DB
authors.lastname: Janssen
authors.forename: H J
authors.initials: HJ
authors.lastname: Busscher
authors.forename: H C
authors.initials: HC
authors.lastname: van der Mei
authors.forename: G
authors.initials: G
authors.lastname: Reid
mesh_terms: D000595:Amino Acid Sequence; D001422:Bacterial Adhesion / Q000187:drug effects*; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000502:physiology; D005260:Female; D052776:Female Urogenital Diseases / Q000382:microbiology; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology; D006801:Humans; D016023:Integrins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D007778:Lactobacillus / Q000378:metabolism*; D008969:Molecular Sequence Data; D038981:Receptors, Collagen
chemical_list: D001426:Bacterial Proteins; D016023:Integrins; D038981:Receptors, Collagen; C418866:surface-binding protein, Lactobacillus