title: Insertional knock-out of protein translocation systems common for Yersinia enterocolitica and Listeria monocytogenes.
abstract: To carry out efficient insertional mutagenesis in Listeria monocytogenes and to facilitate the characterisation of disrupted genes, a novel derivative of plasmid pACYC 184 was constructed, pLIV virA3, carrying a fragment from the virA region of the of Y. enterocolitica plasmid pYVe 0:9. After transformation of this plasmid into L. monocytogenes it was possible to select for its integration into the host DNA at 42 degrees C. Insertional mutants of L. monocytogenes obtained by using pLIV vector containing plasmid DNA fragments from Y. enterocolitica were constructed and are described.
authors.affiliation: Institute of Microbiology, Warsaw University, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Wiśniewski
authors.forename: J
authors.initials: J
authors.lastname: Hrebenda
authors.forename: J
authors.initials: J
authors.lastname: Bielecki
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D017353:Gene Deletion; D006801:Humans; D008089:Listeria monocytogenes / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D016254:Mutagenesis, Insertional; D010957:Plasmids / Q000235:genetics*; D014169:Transformation, Bacterial / Q000235:genetics; D037521:Virulence Factors; D015008:Yersinia enterocolitica / Q000235:genetics* / Q000472:pathogenicity
chemical_list: D001426:Bacterial Proteins; D037521:Virulence Factors