title: Haemoglobin adducts of aromatic amines: diamines and polyaromatic amines.
abstract: Aromatic amines and nitroarenes are important antioxidants and intermediates in the synthesis of dyes, pesticides and plastics. In the present paper we introduce methods for the synthesis of deuterated standards: 3-[2H8]aminofluoranthene, 3,3'-dimethyl-[2H4]benzidine, [2H4]benzidine, N'-acetyl-[2H4]benzidine, 2,4-[2H6]toluenediamine, 2,6-[2H6]toluenediamine. These standards have been used for the quantification of haemoglobin adducts of diamines and polyaromatic amines. Haemoglobin was hydrolysed in 0.1 M sodium hydroxide and the hydrolysate extracted with dichloromethane. The extracts were derivatised with heptafluorobutyric anhydride and analysed by GC-MS with negative chemical ionisation. In one run up to 15 aromatic amines can be determined: 6-aminochrysene, 3-aminofluoranthene, 2-aminofluorene, 1-aminopyrene, benzidine, 3,3'-dichlorobenzidine, 3,3'-dimethoxybenzidine, 3,3'-dimethylbenzidine, 3,3'-methylenedianiline, 4,4'-methylenedianiline, N'-acetyl-benzidine, N'-acetyl-4,4'-methylenedianiline, 4,4'-methylene bis(2-chloroaniline), 2,4-toluenediamine and 2,6-toluenediamine.
authors.affiliation: Walther-Straub-Institut für Pharmakologie und Toxikologie, Ludwig-Maximilians-Universität München, Munich, Germany. gabriele.sabbioni@t-online.de
authors.forename: G
authors.initials: G
authors.lastname: Sabbioni
authors.forename: A
authors.initials: A
authors.lastname: Beyerbach
mesh_terms: D000588:Amines / Q000737:chemistry*; D002138:Calibration; D008401:Gas Chromatography-Mass Spectrometry; D006454:Hemoglobins / Q000737:chemistry*; D013058:Mass Spectrometry; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000588:Amines; D006454:Hemoglobins