title: Contraction-induced injury to single permeabilized muscle fibers from mdx, transgenic mdx, and control mice.
abstract: Muscle fibers of mdx mice that lack dystrophin are more susceptible to contraction-induced injury, particularly when stretched. In contrast, transgenic mdx (tg-mdx) mice, which overexpress dystrophin, show no morphological or functional signs of dystrophy. Permeabilization disrupts the sarcolemma of fibers from muscles of mdx, tg-mdx, and control mice. We tested the null hypothesis stating that, after single stretches of maximally activated single permeabilized fibers, force deficits do not differ among fibers from extensor digitorum longus muscles of mdx, tg-mdx, or control mice. Fibers were maximally activated by Ca(2+) (pCa 4.5) and then stretched through strains of 10%, 20%, or 30% of fiber length (L(f)) at a velocity of 0.5 L(f)/s. Immediately after each strain, the force deficits were not different for fibers from each of the three groups of mice. When collated with studies of membrane-intact fibers in whole muscles of mdx, tg-mdx, and control mice, these results indicate that dystrophic symptoms do not arise from factors within myofibrils but, rather, from disruption of the sarcolemmal integrity that normally provides protection from contraction-induced injury.
authors.affiliation: Institute of Gerontology, University of Michigan, Ann Arbor, Michigan 48109-2007, USA. g.lynch@physiology.unimelb.edu.au
authors.forename: G S
authors.initials: GS
authors.lastname: Lynch
authors.forename: J A
authors.initials: JA
authors.lastname: Rafael
authors.forename: J S
authors.initials: JS
authors.lastname: Chamberlain
authors.forename: J A
authors.initials: JA
authors.lastname: Faulkner
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D002463:Cell Membrane Permeability / Q000235:genetics*; D016189:Dystrophin / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D008822:Mice, Transgenic; D009119:Muscle Contraction / Q000235:genetics; D018485:Muscle Fibers, Skeletal / Q000187:drug effects / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000503:physiopathology*; D009137:Muscular Dystrophy, Animal / Q000503:physiopathology*; D012508:Sarcolemma / Q000235:genetics / Q000378:metabolism; D013314:Stress, Mechanical
chemical_list: D016189:Dystrophin; D002118:Calcium