title: Binding of glycoglycerolipid derived from membranes of Acholeplasma laidlawii PG8 and synthetic analogues to lymphoid cells.
abstract: A component that binds to human lymphoid cells was isolated from the membranes of Acholeplasma laidlawii PG8. The component was extracted using the Bligh-Dyer method and purified using a silica-gel column and TLC. The active component was identified as 3-O:-[2'-O-(alpha-D-glucopyranosyl)- 6'-O-acyl-alpha-D-glucopyranosyl]-1,2-di-O- acyl-sn-glycerol (GAGDG) using (1)H- and (13)C-NMR and GC-MS. The compositions of the major saturated fatty acids were nC (14) (17.8%), isoC(14) (10.7%) and nC (16) (34.9%) as determined by GC-MS. The amounts of unsaturated species were less than 10% of those of the corresponding saturated acids. GAGDGs which have three tetradecanoyl groups were synthesized. These synthetic GAGDGs, as well as GAGDGs derived from A. laidlawii membranes, had a high binding affinity for MOLT-4 and HUT-78 (human T cell lines), Raji (a B cell line), HL-60 (a monoblastoid cell line) and primary cultured human T cells. The binding affinities of GAGDGs with an isoC(14) acyl group was higher than those with nC(14) and nC(16) acyl groups. The binding to lymphoid cells reveals a novel biological activity of GAGDGs.
authors.affiliation: Department of Bacteriology, Kurume University School of Medicine, 67 Asahi-machi, Kurume 830-0011, Japan1.
authors.forename: Saori
authors.initials: S
authors.lastname: Toujima
authors.affiliation: Department of Bacteriology, Kurume University School of Medicine, 67 Asahi-machi, Kurume 830-0011, Japan1.
authors.forename: Koichi
authors.initials: K
authors.lastname: Kuwano
authors.affiliation: Department of Bacteriology, Kurume University School of Medicine, 67 Asahi-machi, Kurume 830-0011, Japan1.
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Bacteriology, Kurume University School of Medicine, 67 Asahi-machi, Kurume 830-0011, Japan1.
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Fujimoto
authors.affiliation: Department of Chemistry, Graduate School of Science, Tohoku University Aoba, Aramaki aza, Aoba-ku, Sendai 980-8578, Japan2.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Hirama
authors.affiliation: Department of Chemistry, Graduate School of Science, Tohoku University Aoba, Aramaki aza, Aoba-ku, Sendai 980-8578, Japan2.
authors.forename: Tohru
authors.initials: T
authors.lastname: Oishi
authors.affiliation: Department of Chemistry, Graduate School of Science, Tohoku University Aoba, Aramaki aza, Aoba-ku, Sendai 980-8578, Japan2.
authors.forename: Sumiko
authors.initials: S
authors.lastname: Fukuda
authors.affiliation: Department of Chemistry, Graduate School of Science, Tohoku University Aoba, Aramaki aza, Aoba-ku, Sendai 980-8578, Japan2.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Nagumo
authors.affiliation: Department of Chemistry, Graduate School of Science, Tohoku University Aoba, Aramaki aza, Aoba-ku, Sendai 980-8578, Japan2.
authors.forename: Hiroto
authors.initials: H
authors.lastname: Imai
authors.affiliation: Department of Chemistry, Graduate School of Science, Tohoku University Aoba, Aramaki aza, Aoba-ku, Sendai 980-8578, Japan2.
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Kikuchi
authors.affiliation: Department of Bacteriology, Kurume University School of Medicine, 67 Asahi-machi, Kurume 830-0011, Japan1.
authors.forename: Sumio
authors.initials: S
authors.lastname: Arai
mesh_terms: D000128:Acholeplasma laidlawii / Q000737:chemistry*; D001422:Bacterial Adhesion; D002240:Carbohydrate Sequence; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry; D006017:Glycolipids / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D007963:Leukocytes, Mononuclear; D008214:Lymphocytes / Q000378:metabolism*; D008969:Molecular Sequence Data
chemical_list: D006017:Glycolipids