Quantitative determination of endocrine- disrupting polychlorinated biphenyls and organochlorinated pesticides in human serum using gas chromatography with electron-capture detection and tandem mass spectrometry.
A sensitive, selective and reliable procedure was developed and validated to determine organochlorinated compounds, which have endocrine-disrupting effects, in human serum. Target compounds were selected between polychlorinated biphenyls and organochlorinated pesticides. Sample workup consisted of (1) extraction of serum with organic solvents, (2) clean-up of the organic extract using acid treatment with H(2)SO(4), (3) elution of the cleaned-up extract through a liquid column chromatographic system and (4) analysis of the fraction eluted by gas chromatography with electron capture detection (ECD) and tandem mass spectrometry (MS/MS) detection. Performance characteristics, such as linearity, sensitivity, precision, accuracy and recovery, of both chromatographic methods were studied. The proposed analytical methodology was applied to determine the target compounds in serum samples from women living in agricultural areas of Almería (Spain). The results show the advantage of MS/MS over ECD in the analysis of real human serum samples where matrix interferences can be confused with target pesticides.
Frenich
A G
AG
Department of Analytical Chemistry, University of Almería, 04071 Almería, Spain.
Vidal
J L
JL
Frías
M M
MM
Olea-Serrano
F
F
Olea
N
N
D001774:Blood Chemical Analysis / Q000379:methods*; D007631:Chlordecone / Q000097:blood / Q000633:toxicity; D004702:Endocrine Glands / Q000187:drug effects; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D007306:Insecticides / Q000097:blood* / Q000633:toxicity; D008731:Methoxychlor / Q000097:blood / Q000633:toxicity; D008917:Mirex / Q000097:blood / Q000633:toxicity; D011078:Polychlorinated Biphenyls / Q000097:blood* / Q000633:toxicity; D013030:Spain
D007306:Insecticides; D011078:Polychlorinated Biphenyls; D007631:Chlordecone; D008731:Methoxychlor; D008917:Mirex