Subset-specific regulation of the lymphatic exit of recirculating lymphocytes in vivo.
The blood-to-lymph recirculation of lymphocytes is required for the maintenance of immune surveillance and the dissemination of memory. Although the ability of lymph-borne cells to recirculate has been well documented, relatively less is known about the migration capacity of PBLs. We have found a clear preference for PBLs to recirculate through s.c. rather than intestinal lymph nodes. This preference could be directly attributed to the migratory characteristics of gammadelta-T cells. gammadelta-T cells were found to express significantly higher levels of L-selectin than other subsets, suggesting that at least some of this preferential migration could be attributed to their interaction with ligands on vascular endothelium. More detailed experiments showed that gammadelta-T cells migrated through lymph nodes with greater efficiency than alphabeta T cells or B cells, which clearly indicated an enhanced ability of gammadelta-T cells to exit lymph nodes in the efferent lymph independent of entry from the blood. This hypothesis was supported by histological examination, where gammadelta-T cells were found almost exclusively in the interfollicular traffic areas within lymph nodes. These data indicate that gammadelta-T cells are the most active recirculating lymphocyte subset in ruminants and suggest new mechanisms to regulate the traffic of lymphocyte subsets through normal lymph nodes.
Young
A J
AJ
Basel Institute for Immunology, Basel, Switzerland. Ayoung@partners.org
Marston
W L
WL
Dudler
L
L
D000818:Animals; D000954:Antigens, Surface / Q000096:biosynthesis; D001775:Blood Circulation / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000502:physiology; D002465:Cell Movement / Q000276:immunology*; D008196:Lymph / Q000166:cytology* / Q000276:immunology / Q000502:physiology*; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology / Q000502:physiology; D018655:Lymphocyte Count; D016131:Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000096:biosynthesis; D012756:Sheep; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism / Q000502:physiology
D000954:Antigens, Surface; D016692:Receptors, Antigen, T-Cell, gamma-delta