Real-time imaging of two-photon-induced fluorescence with a microlens-array scanner and a regenerative amplifier.
A real-time two-photon fluorescence microscope with a microlens-array scanner using a regenerative amplifier is presented. The regenerative amplifier generates pulsed laser lights with extremely high peak power and produces brighter two-photon-induced fluorescence compared with that produced without the amplifier. Experimental results obtained from the observation of some biological samples with the proposed microscope are given.
Fujita
K
K
Department of Applied Physics, Osaka University, Suita, Japan. fujita@ap.eng.osaka-u.ac.jp
Nakamura
O
O
Kaneko
T
T
Kawata
S
S
Oyamada
M
M
Takamatsu
T
T
D000669:Amplifiers, Electronic; D000814:Aniline Compounds; D000818:Animals; D002118:Calcium / Q000032:analysis*; D002478:Cells, Cultured; D004396:Coloring Agents; D004801:Eosine Yellowish-(YS); D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D009206:Myocardium / Q000737:chemistry / Q000166:cytology*; D009942:Organometallic Compounds; D009943:Organophosphorus Compounds; D051381:Rats; D014966:Xanthenes
D000814:Aniline Compounds; D004396:Coloring Agents; D009942:Organometallic Compounds; D009943:Organophosphorus Compounds; D014966:Xanthenes; C066850:Cascade Blue; C059715:Fluo-3; D002118:Calcium; D004801:Eosine Yellowish-(YS)