title: [Genetic diversity of mtDNA of domestic camels (C. bactrianus) in China].
abstract: In this paper the mitochondrial DNA (mtDNA) of 87 domestic camels from Gansu, Xingjiang and Inner Mongolia were analyzed. Totally 18 restriction endonucleases including Apa I, Ava I, BamH I, Bcl I, Bgl I, Bgl II, Dra I, EcoR I, EcoR V, Hind III, Kpn I, Pvu II, Sac I, Sal I, Sca I, Sma I, Xba I and Xho I were used to investigate the restriction fragment length polymorphism (RFLP) of camel mtDNA through Southern blotting to reveal the genetic diversity within three groups of domestic camel and the genetic relationships among the three domestic groups. The results indicated that the average molecular weight of camel mtDNA is 16.26 +/- 0.28 kb, the camel mtDNA is inherited maternally and no variations and heteroplasmy are found in camel mtDNA. Among the 82 domestic camels, 35 restriction morph and 12 haplotypes were detected, and the value of average sequence variation within the domestic population (pi) is 0.2273%, which showed a relatively rich genetic diversity existed in the domestic camels. From F-test of the haplotype distribution and comparison of the haplotype sharement between the 3 domestic camel groups, it was suggested that there is no obvious genetic divergence and that the rich genetic diversity comes from the variation among individuals instead of the genetic divergence between groups. All domestic camels could be divided into 2 types (A and B) with a significant genetic distance (P = 0.0464).
authors.affiliation: Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Science, China.
authors.forename: J X
authors.initials: JX
authors.lastname: Quan
authors.forename: Y P
authors.initials: YP
authors.lastname: Zhang
authors.forename: J L
authors.initials: JL
authors.lastname: Han
authors.forename: Z M
authors.initials: ZM
authors.lastname: Men
mesh_terms: D000818:Animals; D002162:Camelus / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D014644:Genetic Variation; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D004272:DNA, Mitochondrial