Angiogenic growth factors in human dentine matrix.
The importance of growth factors in mediating the cellular responses to injury in the dentine-pulp complex is well recognized and several growth factors are reportedly sequestered in dentine matrix from where they may be released during repair processes. Local angiogenesis at the injury site appears to be critical for successful pulpal repair. Here, soluble and insoluble matrix fractions were isolated from human dentine and the amounts of several important angiogenic growth factors in these fractions measured by enzyme-linked immunosorbent assay (ELISA). The EDTA-soluble matrix fraction contained high concentrations of platelet-derived growth factor (PDGF-AB), lower concentrations of vascular endothelial growth factor (VEGF), placenta growth factor (PlGF) and fibroblast growth factor (FGF2), and very low concentrations of epidermal growth factor (EGF). No FGF2 or PlGF could be detected in the insoluble matrix fractions, but these fractions contained some VEGF, lower concentrations of PDGF-AB and very low concentrations of EGF. It was concluded that dentine matrix contains angiogenic growth factors and that their release from the matrix after injury could make an important contribution to the overall reparative response of the dentine-pulp complex.
Roberts-Clark
D J
DJ
Oral Biology, School of Dentistry, University of Birmingham, St. Chad's Queensway, B4 6NN, Birmingham, UK.
Smith
A J
AJ
D043925:Angiogenesis Inducing Agents / Q000032:analysis* / Q000378:metabolism; D046508:Culture Techniques; D003782:Dental Pulp / Q000737:chemistry / Q000378:metabolism; D003804:Dentin / Q000737:chemistry* / Q000378:metabolism; D016228:Endothelial Growth Factors / Q000032:analysis; D004815:Epidermal Growth Factor / Q000032:analysis; D016222:Fibroblast Growth Factor 2 / Q000032:analysis; D006133:Growth Substances / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D008222:Lymphokines / Q000032:analysis; D000072483:Placenta Growth Factor; D010982:Platelet-Derived Growth Factor / Q000032:analysis; D011257:Pregnancy Proteins / Q000032:analysis; D020033:Protein Isoforms / Q000032:analysis; D011994:Recombinant Proteins; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D014945:Wound Healing
D043925:Angiogenesis Inducing Agents; D016228:Endothelial Growth Factors; D006133:Growth Substances; D008222:Lymphokines; C000608036:PGF protein, human; D010982:Platelet-Derived Growth Factor; D011257:Pregnancy Proteins; D020033:Protein Isoforms; D011994:Recombinant Proteins; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; C095477:platelet-derived growth factor AB; D016222:Fibroblast Growth Factor 2; D000072483:Placenta Growth Factor; D004815:Epidermal Growth Factor