title: Morphological changes in rabbit corneal endothelium after surgical injury.
abstract: OBJECTIVE
  To understand the responses of the corneal endothelium to different types of surgical insults, we chronologically investigated morphologic changes in rabbit endothelial cells after injury.
  
  
  METHODS
  We performed a mechanical incision, epithelial ablation, or excimer laser irradiation on rabbit corneas and observed the changes in the endothelial cells for up to 2 weeks after surgery under a light microscope and an electron microscope.
  
  
  RESULTS
  Although we observed enlargements of intercellular spaces between neighboring endothelial cells, intercellular adhesion complexes were maintained and the cells joined tightly near their apexes immediately after each procedure. We observed no signs of endothelial cell degeneration after the procedures, but we did observe many Golgi apparati, rough-surfaced endoplasmic reticula, and secreted granules, indicating that the cells had been activated. After each procedure, varying periods of time were required before the intercellular junctions and spaces returned to normal.
  
  
  CONCLUSIONS
  These results suggest that the different kinds of surgical injuries affected the corneal endothelium in different ways, but that the early changes were reversible.
authors.affiliation: Department of Ophthalmology, Yamaguchi University School of Medicine, Ube, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Miyazaki
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
authors.forename: T
authors.initials: T
authors.lastname: Nishida
mesh_terms: D000818:Animals; D018870:Endoplasmic Reticulum, Rough / Q000648:ultrastructure; D004728:Endothelium, Corneal / Q000601:surgery / Q000648:ultrastructure*; D005500:Follow-Up Studies; D006056:Golgi Apparatus / Q000648:ultrastructure; D007646:Keratotomy, Radial; D054018:Lasers, Excimer; D008297:Male; D008928:Mitochondria / Q000648:ultrastructure; D018903:Photorefractive Keratectomy; D011817:Rabbits; D014617:Vacuoles / Q000648:ultrastructure; D014945:Wound Healing