title: Construction of transgenic pea lines with modified expression of diamine oxidase and modified nodulation responses with exogenous putrescine.
abstract: Diamine oxidase (DAO) might influence pea nodule development either by regulating the peroxide-driven cross-linking of glycoproteins in the infection thread matrix or by modifying the metabolism of diamines and polyamines in host cells. Transformed lines of pea (Pisum sativum) with the coding sequence for DAO (PSAO-1) in sense orientation behind a tissue-specific promoter (pENOD12A) showed strong co-suppression of DAO activity in extracts from nodules and epicotyls, whereas the antisense constructs were relatively unaffected. No difference in nodule number was observed between transformed lines and controls, suggesting that DAO does not normally have an essential role in nodule initiation. However, lines showing co-suppression of DAO were less sensitive to the inhibitory effects of exogenous putrescine and less active in the cross-linking of matrix glycoprotein, indicating that putrescine-derived products of DAO activity could retard nodule development. Inoculation of co-suppressed lines with Rhizobium strain B661 (a lipopolysaccharide-defective mutant) resulted in more extreme impairment of nodule development and nitrogen fixation capacity, relative to lines with normal levels of DAO, which suggests that DAO may serve to reduce the endogenous level of inhibitory diamines or polyamines in nodules under physiological stress. We conclude that the most critical role of DAO in pea nodule development is apparently in the regulation of diamine levels in host tissues.
authors.affiliation: John Innes Centre, Norwich, UK.
authors.forename: J P
authors.initials: JP
authors.lastname: Wisniewski
authors.forename: N J
authors.initials: NJ
authors.lastname: Brewin
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D009586:Nitrogen Fixation / Q000187:drug effects*; D018532:Pisum sativum / Q000201:enzymology / Q000235:genetics* / Q000502:physiology; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics* / Q000502:physiology; D011700:Putrescine / Q000494:pharmacology*
chemical_list: D017931:DNA Primers; D006631:Amine Oxidase (Copper-Containing); D011700:Putrescine