title: Specificity of mutations induced by the food-associated heterocyclic amine 2-amino-1-methyl-6-phenylimidazo-[4,5-b]-pyridine in colon cancer cell lines defective in mismatch repair.
abstract: Recently, we have shown a hypermutable response to the food-associated heterocyclic amine 2-amino-1-methyl-6-phenylimidazo-[4,5-b]-pyridine (PhIP) in human cells defective in mismatch repair (MMR). These findings suggest that exogenous compounds such as PhIP may play an important role in the generation of tumors in MMR-defective individuals. The specificity of mutations induced by PhIP exposure at the endogenous HPRT locus was determined in cell lines defective in MMR to better understand the mutagenic effects of PhIP in MMR-defective individuals and to gain insight into the molecular mechanism of carcinogenesis induced by PhIP. Eighty-six induced HPRT mutants from two different cell lines were isolated and sequenced after exposure to 10 microM PhIP. Nineteen (22%) of these mutants contained G:C to T:A transversion mutations, consistent with the promutagenic adduct of PhIP at the C8 position of guanine miscoding with adenine. This level of PhIP-induced G:C to T:A transversions was approximately 4.5-fold higher than spontaneous G:C to T:A frequencies. Additionally, a hotspot for mutation was observed in a run of six guanines in HPRT exon 3, where a total of 23 (27%) of all PhIP-induced mutations occurred. These mutations consisted of transversions, transitions, and frameshift mutations. The increase in mutant frequency at this run of guanines corresponded to a 24-fold elevation above the spontaneous frequency in one cell line and a 3.3-fold increase in the other. These data suggest that PhIP may increase the risk of human carcinogenesis mediated by MMR by increasing mutations at runs of guanine residues. PhIP may thereby promote tumorigenesis by mutating growth-regulating genes that contain runs of guanines in their coding sequences, such as BAX, the insulin-like growth factor II receptor IGFIIR, and even the mismatch repair gene hMSH6.
authors.affiliation: Merck Research Laboratories, Department of Genetic and Cellular Toxicology, West Point, Pennsylvania 19486, USA. warren_glaab@merck.com
authors.forename: W E
authors.initials: WE
authors.lastname: Glaab
authors.forename: K L
authors.initials: KL
authors.lastname: Kort
authors.forename: T R
authors.initials: TR
authors.lastname: Skopek
mesh_terms: D020137:Base Pair Mismatch / Q000502:physiology*; D002273:Carcinogens / Q000633:toxicity; D003043:Cocarcinogenesis; D003110:Colonic Neoplasms / Q000235:genetics*; D004252:DNA Mutational Analysis; D004260:DNA Repair / Q000502:physiology*; D004273:DNA, Neoplasm / Q000235:genetics; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D007093:Imidazoles / Q000633:toxicity*; D009153:Mutagens / Q000633:toxicity*; D009154:Mutation / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: D002273:Carcinogens; D004273:DNA, Neoplasm; D007093:Imidazoles; D009153:Mutagens; C049584:2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine; D007041:Hypoxanthine Phosphoribosyltransferase