Coupling high-pressure MALDI with ion mobility/orthogonal time-of-flight mass spectrometry.
A new ion mobility/time-of-flight mass spectrometer employing a high-pressure MALDI source has been designed and tested. The prototype instrument operates at a source/drift cell pressure of 1-10 Torr helium, resulting in a mobility resolution of approximately 25. A small time-of-flight mass spectrometer (20 cm) with a mass resolution of up to 200 has been attached to the drift cell to identify (in terms of mass-to-charge ratio) the separated ions. A simple tripeptide mixture has been separated in the drift tube and mass identified as singly protonated species. The ability to separate peptide mixtures, e.g., tryptic digest of a protein, is illustrated and compared to results obtained on a high-vacuum time-of-flight instrument.
Gillig
K J
KJ
Department of Chemistry, Texas A&M University, College Station 77843, USA.
Ruotolo
B
B
Stone
E G
EG
Russell
D H
DH
Fuhrer
K
K
Gonin
M
M
Schultz
A J
AJ
D000595:Amino Acid Sequence; D013058:Mass Spectrometry / Q000295:instrumentation*; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D010455:Peptides