[The effect of oxygen supply on production of glycerol with Candida glycerolgenesis].
The relationship between oxygen supply and production of glycerol or byproduct with Candida glycerolgenesis have been studied. The experiments in shake flasks indicated that the biomass was determined by the concentration of corn steep liquor when other fermentation condition was constant, the concentration of corn steep liquor and the ratio of the volume of the medium to the capacity of the flask affected the yield of glycerol and other byproducts because of different oxygen supply. With 0.4% corn steep liquid and the ratio of the volume of the medium to the capacity of the flask was 0.08, the yeast yielded higher level of glycerol concentration and little other byproducts, while oxygen was insufficient for glycerol production such as the ratio was 0.24, the yeast would produce much more alcohol and other byproduct, then decreased the yield of glycerol. In the fermentation process of 5 L fermentor, agitator speed mostly affected the DO level in the medium. In growth phase the specific oxygen consumption rate of C. glycerolgenesis was 28 mg/(g.h), and during fermentation process it was 16 mg/(g.h). With suitable oxygen supply, C. glycerolgenesis can produce high level of glycerol whereas ethanol and other byproducts was nearly zero.
Jin
H R
HR
School of Biotechnology, Wuxi University of Light Industry.
Fang
H Y
HY
Zhuge
J
J
D002175:Candida / Q000378:metabolism*; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism*; D010100:Oxygen / Q000494:pharmacology*
D005990:Glycerol; D010100:Oxygen