title: Modeling axonal injury in vitro: injury and regeneration following acute neuritic trauma.
abstract: Traumatic injury to axons was modeled in vitro using sympathetic principal neurons from the rat superior cervical ganglion. Neurons were grown as a pure culture on collagen in parallel tracks, with cell somata confined to the center, and neurites occupying the periphery of the culture dish. Growing as fascicles on tracks, the neurites demonstrated periodic varicosities. Neuritic transection was reliably and reproducibly achieved with a motor driven rubber impactor injury device. During a period lasting at least 1 h, dieback involving the proximal neurites averaged 105 +/- 10 microm. This was followed by neurite regeneration, with the injured segment being traversed within 36 h at an average rate of regeneration of 595 +/- 15 microm/day. The distal neurite segments showed degenerative changes within 1 h following transection, with initial receding of neurites progressing to vacuolation, beading, blebbing, and eventual detachment from the underlying matrix. This in vitro model of axonal injury allows neuritic injury to be studied at the cellular and molecular levels, and also provides a unique opportunity to test potential neuromodulatory and neuroprotective strategies.
authors.affiliation: Playfair Neuroscience Unit, Toronto Western Hospital and University of Toronto, McL-Pav 12-423, 339 Bathurst Street, Toronto, Ontario M5T-2S8, Canada.
authors.forename: I
authors.initials: I
authors.lastname: Fayaz
authors.forename: C H
authors.initials: CH
authors.lastname: Tator
mesh_terms: D000208:Acute Disease; D000818:Animals; D000831:Animals, Newborn; D019771:Axotomy; D018929:Cell Culture Techniques; D048429:Cell Size / Q000502:physiology; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005728:Ganglia, Sympathetic / Q000293:injuries* / Q000473:pathology / Q000503:physiopathology; D009410:Nerve Degeneration / Q000473:pathology / Q000503:physiopathology*; D009416:Nerve Regeneration / Q000502:physiology*; D016501:Neurites / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D051381:Rats; D013119:Spinal Cord Injuries / Q000473:pathology / Q000503:physiopathology; D013997:Time Factors