Increment of sister chromatid exchange frequencies (SCE) due to epichlorohydrin (ECH) in vitro treatment in human lymphocytes.
Although several studies have examined the effects on health of exposure to epichlorohydrin (ECH) through normal industrial operations and production, there is still considerable interest in its potential harmful effects on humans. The aim of the present study was to evaluate ECH effects in vitro through controlled investigations by using sister chromatid exchange (SCE), micronucleus (MN), and chromosome aberrations (CA) as the test battery. Cultures for cytogenetic tests were set up from blood samples of four healthy non-smoking and three smoking males. The experiments were performed using four different concentrations: 10(-10) M, 10(-8) M, 10(-6) M, and 10(-4) M, of ECH in DMSO. Analysis of variance showed that concentrations of ECH had significant effects on SCE/cell frequencies in the lymphocyte cultures of all donors (F=100.25, P<0.001). We were unable to find any evidence of significant increases in CA and MN frequencies in ECH-treated lymphocyte cultures with respect to the controls.
Bukvic
N
N
Dipartimento di Medicina Interna e Medicina Pubblica (DIMIMP), Sezione di Genetica Medica, Università degli Studi di Bari, Bari, Italy.
Bavaro
P
P
Soleo
L
L
Fanelli
M
M
Stipani
I
I
Elia
G
G
Susca
F
F
Guanti
G
G
D000328:Adult; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D004305:Dose-Response Relationship, Drug; D004811:Epichlorohydrin / Q000633:toxicity*; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000473:pathology; D008297:Male; D015162:Micronucleus Tests; D009153:Mutagens; D012044:Regression Analysis; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D009153:Mutagens; D004811:Epichlorohydrin