title: Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen.
abstract: Pseudomonas aeruginosa is a ubiquitous environmental bacterium that is one of the top three causes of opportunistic human infections. A major factor in its prominence as a pathogen is its intrinsic resistance to antibiotics and disinfectants. Here we report the complete sequence of P. aeruginosa strain PAO1. At 6.3 million base pairs, this is the largest bacterial genome sequenced, and the sequence provides insights into the basis of the versatility and intrinsic drug resistance of P. aeruginosa. Consistent with its larger genome size and environmental adaptability, P. aeruginosa contains the highest proportion of regulatory genes observed for a bacterial genome and a large number of genes involved in the catabolism, transport and efflux of organic compounds as well as four potential chemotaxis systems. We propose that the size and complexity of the P. aeruginosa genome reflect an evolutionary adaptation permitting it to thrive in diverse environments and resist the effects of a variety of antimicrobial substances.
authors.affiliation: PathoGenesis Corporation, Seattle, Washington 98119, USA.
authors.forename: C K
authors.initials: CK
authors.lastname: Stover
authors.forename: X Q
authors.initials: XQ
authors.lastname: Pham
authors.forename: A L
authors.initials: AL
authors.lastname: Erwin
authors.forename: S D
authors.initials: SD
authors.lastname: Mizoguchi
authors.forename: P
authors.initials: P
authors.lastname: Warrener
authors.forename: M J
authors.initials: MJ
authors.lastname: Hickey
authors.forename: F S
authors.initials: FS
authors.lastname: Brinkman
authors.forename: W O
authors.initials: WO
authors.lastname: Hufnagle
authors.forename: D J
authors.initials: DJ
authors.lastname: Kowalik
authors.forename: M
authors.initials: M
authors.lastname: Lagrou
authors.forename: R L
authors.initials: RL
authors.lastname: Garber
authors.forename: L
authors.initials: L
authors.lastname: Goltry
authors.forename: E
authors.initials: E
authors.lastname: Tolentino
authors.forename: S
authors.initials: S
authors.lastname: Westbrock-Wadman
authors.forename: Y
authors.initials: Y
authors.lastname: Yuan
authors.forename: L L
authors.initials: LL
authors.lastname: Brody
authors.forename: S N
authors.initials: SN
authors.lastname: Coulter
authors.forename: K R
authors.initials: KR
authors.lastname: Folger
authors.forename: A
authors.initials: A
authors.lastname: Kas
authors.forename: K
authors.initials: K
authors.lastname: Larbig
authors.forename: R
authors.initials: R
authors.lastname: Lim
authors.forename: K
authors.initials: K
authors.lastname: Smith
authors.forename: D
authors.initials: D
authors.lastname: Spencer
authors.forename: G K
authors.initials: GK
authors.lastname: Wong
authors.forename: Z
authors.initials: Z
authors.lastname: Wu
authors.forename: I T
authors.initials: IT
authors.lastname: Paulsen
authors.forename: J
authors.initials: J
authors.lastname: Reizer
authors.forename: M H
authors.initials: MH
authors.lastname: Saier
authors.forename: R E
authors.initials: RE
authors.lastname: Hancock
authors.forename: S
authors.initials: S
authors.lastname: Lory
authors.forename: M V
authors.initials: MV
authors.lastname: Olson
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D019295:Computational Biology; D004269:DNA, Bacterial; D004352:Drug Resistance, Microbial; D015964:Gene Expression Regulation, Bacterial; D016680:Genome, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D011552:Pseudomonas Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial