title: Platelet alloantigen frequencies in Amazon Indians and Brazilian blood donors.
abstract: The frequencies of human platelet-specific alloantigens (HPAs) vary between different ethnic groups, and genotyping using DNA techniques has been preferred over immunophenotyping methods for population studies. Using a polymerase chain reaction with allele-specific primers (PCR-ASP) method, we determined the allelic polymorphisms of five HPA systems among 174 unrelated individuals of two different Brazilian ethnic groups including Amazon Indians (n = 95) and blood donors (n = 79). Comparison of the calculated gene frequencies of the two alleles of HPA-1, -2, -3, -4 and -5 systems for Amazon Indians and Brazilian blood donors showed that gene frequencies obtained for the two alleles of HPA-1 (P<0.001), HPA-2 (P = 0.001) and HPA-5 (P<0.001) were significantly different between the two groups of individuals. All natives tested carried the HPA-2a and the HPA-5a alleles, but the HPA-1b and HPA-4b alleles are absent from the Indian population. It was also observed that all blood donors carried the HPA-1a, HPA-4a and HPA-5a alleles. In conclusion, the present data indicate differences in the frequency of the HPA systems between Amazon Indians and Brazilian subjects who present a high rate of racial admixture. While the frequencies of the HPA-1 and HPA-5 genes seen in Amazon Indians are similar to those reported for Oriental populations, the frequencies of the HPAs alleles in Brazilian blood donors are comparable to those reported for populations in North America and Europe.
authors.affiliation: Hematology and Transfusion Medicine Service, Universidade Federal de São Paulo, Escola Paulista de Medicina, São Paulo, SP, Brazil.
authors.forename: A K
authors.initials: AK
authors.lastname: Chiba
authors.forename: J O
authors.initials: JO
authors.lastname: Bordin
authors.forename: S T
authors.initials: ST
authors.lastname: Kuwano
authors.forename: M S
authors.initials: MS
authors.lastname: Figueiredo
authors.forename: K I
authors.initials: KI
authors.lastname: Carvalho
authors.forename: J P
authors.initials: JP
authors.lastname: Vieira-Filho
authors.forename: J
authors.initials: J
authors.lastname: Kerbauy
mesh_terms: D000483:Alleles; D016824:Antigens, Human Platelet / Q000097:blood / Q000235:genetics*; D001782:Blood Donors; D001938:Brazil / Q000453:epidemiology; D005006:Ethnicity / Q000235:genetics; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007199:Indians, South American / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic
chemical_list: D016824:Antigens, Human Platelet