Prevalence of non-O157:H7 shiga toxin-producing Escherichia coli in diarrheal stool samples from Nebraska.
We determined the prevalence of Shiga toxin-producing Escherichia coli (STEC) in diarrheal stool samples from Nebraska by three methods: cefixime-tellurite sorbitol MacConkey (CT- SMAC) culture, enterohemorrhagic E. coli (EHEC) enzyme immunoassay, and stx1,2 polymerase chain reaction (PCR). Fourteen (4.2%) of 335 specimens were positive by at least one method (CT-SMAC culture [6 of 14], EHEC enzyme immunoassay [13 of 14], stx1,2 PCR [14 of 14]). Six contained serogroup O157, while non-O157 were as prevalent as O157 serogroups.
Fey
P D
PD
University of Nebraska Medical Center, Omaha, Nebraska 68198-5400, USA.
Wickert
R S
RS
Rupp
M E
ME
Safranek
T J
TJ
Hinrichs
S H
SH
D003967:Diarrhea / Q000453:epidemiology* / Q000382:microbiology*; D019453:Escherichia coli O157 / Q000145:classification* / Q000378:metabolism / Q000472:pathogenicity; D005243:Feces / Q000382:microbiology; D006801:Humans; D007124:Immunoenzyme Techniques; D009329:Nebraska / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012703:Serotyping / Q000379:methods*; D022621:Shiga Toxin / Q000096:biosynthesis / Q000302:isolation & purification*
D022621:Shiga Toxin