title: IS1675, a novel lactococcal insertion element, forms a transposon-like structure including the lacticin 481 lantibiotic operon.
abstract: Two copies of IS1675, a novel lactococcal insertion element from the IS4 family, are present on a 70-kb plasmid, where they frame the lantibiotic lacticin 481 operon. The whole structure could be a composite transposon designated Tn5721. This study shows that the lacticin 481 operon does not include any regulatory gene and provides a new example of a transposon-associated bacteriocin determinant. We identified five other IS1675 copies not associated with the lacticin 481 operon. The conservation of IS1675 flanking sequences suggested a 24-bp target site.
authors.affiliation: Laboratoire de Biologie et Chimie Moléculaires, EA 2594, Université de Bretagne Sud, Vannes, France. alain.dufour@univ-ubs.fr
authors.forename: A
authors.initials: A
authors.lastname: Dufour
authors.forename: A
authors.initials: A
authors.lastname: Rincé
authors.forename: P
authors.initials: P
authors.lastname: Uguen
authors.forename: J P
authors.initials: JP
authors.lastname: Le Pennec
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins / Q000235:genetics; D001430:Bacteriocins; D001483:Base Sequence; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D013294:Lactococcus lactis / Q000235:genetics*; D008969:Molecular Sequence Data; D009876:Operon; D017422:Sequence Analysis, DNA
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001430:Bacteriocins; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; C082508:lacticin 481