title: Staphylococcus aureus causes acute inflammatory episodes in the cornea during contact lens wear.
abstract: The aim of this investigation was to determine the ability of Staphylococcus aureus to cause keratitis during contact lens wear in the rabbit. Rabbits were fitted with hydrogel lenses and wore them on an extended wear schedule for 7 weeks. At the end of each week of wear, one drop of S. aureus was added to the eyes, and the eyes were monitored using slit lamp bio-microscopy, with subsequent microbial and histological studies of the cornea. In the presence of S. aureus, keratitis occurred after 3 weeks of lens wear, a condition that was characterized by diffuse infiltration of the peripheral cornea and limbal redness. In the absence of bacteria, there was no infiltration until week 6. Contact lens wear also prolonged the retention of bacteria in the eye. Extended contact lens wear and S. aureus caused non-ulcerative keratitis in a rabbit model, and disturbances in the ability of the eye to remove bacteria.
authors.affiliation: Cooperative Research Centre for Eye Research and Technology, and Cornea and Contact Lens Research Unit, School of Optometry, University of New South Wales, Sydney, Australia. pwu@cclru.randwick.unsw.edu.au
authors.forename: P Z
authors.initials: PZ
authors.lastname: Wu
authors.forename: A
authors.initials: A
authors.lastname: Thakur
authors.forename: F
authors.initials: F
authors.lastname: Stapleton
authors.forename: M D
authors.initials: MD
authors.lastname: Willcox
mesh_terms: D000208:Acute Disease; D000818:Animals; D015169:Colony Count, Microbial; D003228:Conjunctiva / Q000382:microbiology / Q000473:pathology; D003262:Contact Lenses, Extended-Wear / Q000009:adverse effects*; D003315:Cornea / Q000382:microbiology / Q000473:pathology; D015818:Eye Infections, Bacterial / Q000209:etiology* / Q000382:microbiology / Q000473:pathology; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D007634:Keratitis / Q000209:etiology* / Q000382:microbiology / Q000473:pathology; D011817:Rabbits; D013203:Staphylococcal Infections / Q000209:etiology* / Q000382:microbiology / Q000473:pathology; D013211:Staphylococcus aureus / Q000302:isolation & purification*; D013997:Time Factors
chemical_list: D020136:Hydrogel, Polyethylene Glycol Dimethacrylate