Fatty acid profile of skeletal muscle phospholipids in trained and untrained young men.
Endurance trained (n = 14) and untrained young men (n = 15) were compared regarding the fatty acid profile of the vastus lateralis muscle after 8 wk on diets with a similar fatty acid composition. The skeletal muscle phospholipids in the trained group contained lower proportions of palmitic acid (16:0) (-12.4%, P < 0.001) and di-homo-gamma-linolenic acid [20:3(n-6)] (-15.3%, P = 0.018), a lower n-6-to-n-3 ratio (-42.0%, P = 0.015), higher proportions of stearic acid (18:0) (+9.8%, P = 0.004) and sum of n-3 polyunsaturated fatty acids (+33.8%, P = 0.009), and a higher ratio between 20:4(n-6) to 20:3(n-6) (+18.4%, P = 0.006) compared with those in the untrained group. The group differences in 16:0, 20:3(n-6), 18:0/16:0, and 20:4(n-6)/20:3(n-6) were independent of fiber-type distribution. The trained group also showed a lower proportion of 16:0 (-7.9%, P < 0.001) in skeletal muscle triglycerides irrespective of fiber type. In conclusion, the fatty acid profile of the skeletal muscle differed between trained and untrained individuals, although the dietary fatty acid composition was similar. This difference was not explained by different fiber-type distribution alone but appears to be a direct consequence of changes in fatty acid metabolism due to the higher level of physical activity.
Andersson
A
A
Clinical Nutrition Research Unit, Department of Public Health and Caring Sciences/Geriatrics, Uppsala University, S-751 25 Uppsala, Sweden. agneta.andersson@geriatrik.uu.se
Sjödin
A
A
Hedman
A
A
Olsson
R
R
Vessby
B
B
D000328:Adult; D004032:Diet; D016552:Ergometry; D015444:Exercise; D005227:Fatty Acids / Q000032:analysis*; D006801:Humans; D008297:Male; D018485:Muscle Fibers, Skeletal / Q000166:cytology; D018482:Muscle, Skeletal / Q000737:chemistry*; D010101:Oxygen Consumption / Q000502:physiology; D010743:Phospholipids / Q000737:chemistry*; D010809:Physical Fitness / Q000502:physiology*; D014280:Triglycerides / Q000737:chemistry
D005227:Fatty Acids; D010743:Phospholipids; D014280:Triglycerides