title: ISBst12, a novel type of insertion-sequence element causing loss of S-layer-gene expression in Bacillus stearothermophilus ATCC 12980.
abstract: The cell surface of the surface layer (S-layer)-carrying strain of Bacillus stearothermophilus ATCC 12980 is completely covered with an oblique lattice composed of the S-layer protein SbsC. In the S-layer-deficient strain, theS-layer gene sbsC was still present but was interrupted by a novel type of insertion sequence (IS) element designated ISBst12. The insertion site was found to be located within the coding region of the sbsC gene, 199 bp downstream from the translation start of SbsC. ISBst12 is 1612 bp long, bounded by 16 bp imperfect inverted repeats and flanked by a directly repeated 8 bp target sequence. ISBst12 contains an ORF of 1446 bp and is predicted to encode a putative transposase of 482 aa with a calculated theoretical molecular mass of 55562 Da and an isoelectric point of 9.13. The putative transposase does not exhibit a typical DDE motif but displays aHis-Arg-Tyr triad characteristic of the active site of integrases from the bacteriophage lambda Int family. Furthermore, two overlapping leucine-zipper motifs were identified at the N-terminal part of the putative transposase. As revealed by Southern blotting, ISBst12 was present in multiple copies in the S-layer-deficient strain as well as in the S-layer-carrying strain. Northern blotting indicated that S-layer gene expression is already inhibited at the transcriptional level, since no sbsC-specific transcript could be identified in the S-layer-deficient strain. By using PCR, ISBst12 was also detected in B. stearothermophilus PV72/p6, in its oxygen-induced strain variant PV72/p2 and in the S-layer-deficient strain PV72/T5.
authors.affiliation: Zentrum für Ultrastrukturforschung und Ludwig Bolzmann-Institut für Molekulare Nanotechnologie, Universität für Bodenkultur, A-1180 Vienna, Austria1.
authors.forename: Eva M
authors.initials: EM
authors.lastname: Egelseer
authors.affiliation: Zentrum für Ultrastrukturforschung und Ludwig Bolzmann-Institut für Molekulare Nanotechnologie, Universität für Bodenkultur, A-1180 Vienna, Austria1.
authors.forename: Rughia
authors.initials: R
authors.lastname: Idris
authors.affiliation: Zentrum für Ultrastrukturforschung und Ludwig Bolzmann-Institut für Molekulare Nanotechnologie, Universität für Bodenkultur, A-1180 Vienna, Austria1.
authors.forename: Marina
authors.initials: M
authors.lastname: Jarosch
authors.affiliation: Zentrum für Ultrastrukturforschung und Ludwig Bolzmann-Institut für Molekulare Nanotechnologie, Universität für Bodenkultur, A-1180 Vienna, Austria1.
authors.forename: Thomas
authors.initials: T
authors.lastname: Danhorn
authors.affiliation: Zentrum für Ultrastrukturforschung und Ludwig Bolzmann-Institut für Molekulare Nanotechnologie, Universität für Bodenkultur, A-1180 Vienna, Austria1.
authors.forename: Uwe B
authors.initials: UB
authors.lastname: Sleytr
authors.affiliation: Zentrum für Ultrastrukturforschung und Ludwig Bolzmann-Institut für Molekulare Nanotechnologie, Universität für Bodenkultur, A-1180 Vienna, Austria1.
authors.forename: Margit
authors.initials: M
authors.lastname: Sára
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D015152:Blotting, Northern; D015139:Blotting, Southern; D004251:DNA Transposable Elements; D001411:Geobacillus stearothermophilus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D019895:Transposases / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D008562:Membrane Glycoproteins; C055764:S-layer proteins; D019895:Transposases