Application of magnetite and silica-magnetite composites to the isolation of genomic DNA.
Magnetite and silica-magnetite composites were used as adsorbents for the isolation of genomic DNA from maize kernels. Two methods are described for the preparation of silica-magnetite composites, both of which afford higher yields of genomic DNA than when using magnetite alone, or a commerically available kit. DNA isolated using silica-magnetite was suitable for use in further applications such as polymerase chain reaction amplification and enzyme digestion.
Taylor
J I
JI
Department of Chemical and Life Sciences, University of Greenwich, London, UK.
Hurst
C D
CD
Davies
M J
MJ
Sachsinger
N
N
Bruce
I J
IJ
D004262:DNA Restriction Enzymes / Q000378:metabolism; D018744:DNA, Plant / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D052203:Ferrosoferric Oxide; D007501:Iron / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D012822:Silicon Dioxide / Q000737:chemistry*; D003313:Zea mays / Q000737:chemistry
D018744:DNA, Plant; D010087:Oxides; D012822:Silicon Dioxide; D007501:Iron; D004262:DNA Restriction Enzymes; D052203:Ferrosoferric Oxide