Hypochlorous acid-induced lysis of human erythrocytes. Inhibition of cellular damage by the isoflavonoid genistein-8-C-glucoside.
Erythrocyte damage induced by hypochlorous acid (HOCl) results in cell lysis developing with time after the oxidant is removed (post-hemolysis). The apparent rate constant of post-hemolysis depends on time of incubation in the presence of HOCl and concentration of this oxidant. HOCl-dependent damage of erythrocyte membranes is associated with uncompetitive inhibition of the membrane-bound acetylcholinesterase. Genistein-8-C-glucoside is an isoflavonoid isolated from the flowers of Lupinus luteus L.; in aqueous solution, genistein-8-C-glucoside (0.5-2 mM) efficiently inhibited HOCl-induced damage to erythrocytes similar to the known HOCl scavengers taurine and reduced glutathione. This bioflavonoid can protect the erythrocyte membrane (and to a lesser extent, intraerythrocytic components) by interacting with the reactive chlorine species including hypochlorous acid and membrane-bound chloroamines formed in the reaction of HOCl with erythrocyte membrane proteins.
Zavodnik
L B
LB
Institute of Biochemistry, National Academy of Sciences of Belarus, Grodno, 230017, Belarus. zavodnik@biochem.belpak.grodno.by
Zavodnik
I B
IB
Lapshina
E A
EA
Shkodich
A P
AP
Bryszewska
M
M
Buko
V U
VU
D004347:Drug Interactions; D004912:Erythrocytes / Q000187:drug effects* / Q000502:physiology; D005419:Flavonoids / Q000494:pharmacology; D019833:Genistein / Q000031:analogs & derivatives / Q000494:pharmacology*; D005960:Glucosides / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D006997:Hypochlorous Acid / Q000494:pharmacology*; D066298:In Vitro Techniques; D010084:Oxidation-Reduction / Q000187:drug effects
D005419:Flavonoids; D005960:Glucosides; C415443:genistein-8-c-glucoside; D006997:Hypochlorous Acid; D019833:Genistein