title: The incidence of clonal T-cell receptor rearrangements in B-cell precursor acute lymphoblastic leukemia varies with age and genotype.
abstract: B-cell precursor acute lymphoblastic leukemias (BCP-ALLs) are increasingly treated on risk-adapted protocols based on presenting clinical and biological features. Residual molecular positivity of clonal immunoglobulin (IG) and T-cell receptor (TCR) rearrangements allows detection of patients at an increased risk of relapse. If these rearrangements are to be used for universal follow-up, it is important to determine the extent to which they are informative in different BCP-ALL subsets. We show that IGH V-D-J rearrangements occur in 89% of 163 BCP-ALL, with no significant variation according to age or genotype (BCR-ABL, TEL-AML1, MLL-AF4, and E2A-PBX1). In contrast, TCRG rearrangements, which occur in 60% of patients overall, are frequent in BCR-ABL and TEL-AML1, are less so in MLL-AF4, and are virtually absent in infants aged predominantly from 1 to 2 years and in E2A-PBX1 ALLs. Incidence of the predominant TCRD Vdelta2-Ddelta3 rearrangement decreases with age but is independent of genotype. These differences are not due to differential recombination activating gene activity, nor can they be explained adequately by stage of maturation arrest. Analysis of MLL-AF4 BCP-ALL is in keeping with transformation of a precursor at an early stage of ontogenic development, despite the adult onset of the cases analyzed. We postulate that the complete absence of TCRG rearrangement in E2A-PBX1 cases may result from deregulated E2A function. These data also have practical consequences for the use of TCR clonality for the molecular follow-up of BCP-ALL.
authors.affiliation: Department of Biological and Clinical Hematology, and CNRS UMR8603, Hôpital Necker-Enfants Malades and Université Paris V, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Brumpt
authors.forename: E
authors.initials: E
authors.lastname: Delabesse
authors.forename: K
authors.initials: K
authors.lastname: Beldjord
authors.forename: F
authors.initials: F
authors.lastname: Davi
authors.forename: J M
authors.initials: JM
authors.lastname: Cayuela
authors.forename: C
authors.initials: C
authors.lastname: Millien
authors.forename: P
authors.initials: P
authors.lastname: Villarese
authors.forename: P
authors.initials: P
authors.lastname: Quartier
authors.forename: A
authors.initials: A
authors.lastname: Buzyn
authors.forename: F
authors.initials: F
authors.lastname: Valensi
authors.forename: E
authors.initials: E
authors.lastname: Macintyre
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D001402:B-Lymphocytes / Q000276:immunology / Q000473:pathology; D002051:Burkitt Lymphoma / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D015329:Gene Rearrangement, T-Lymphocyte; D005838:Genotype; D006801:Humans; D007223:Infant; D008875:Middle Aged; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology
chemical_list: D011948:Receptors, Antigen, T-Cell