[Production of L(+)-tartaric acid by immobilized Corynebacterium sp. JZ-1].
Corynebacterium sp. JZ-1 cells, which showed a high enzymatic activity of cis-epoxysuccinate hydrolase, were immobilizde by entrapping in kappa-carrageenan, then treated with some active agents. The recovery of enzyme was over 100%. In shake flask, the stability of immobilized cells was satisfactory after they reacted with disodium cis-epoxysuccinate for 10 batches. The same result was attained in column(1500 L) with the substrate fed in continuously for 90 days. The optimum pH value of the immobilized cells was 9.0, the optimum temperature was 45 degrees C.
Zhang
J G
JG
College of Life Sciences, Zhejiang University, Hangzhou.
Qian
Y J
YJ
D003352:Corynebacterium / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D013644:Tartrates / Q000378:metabolism*; D013696:Temperature
D013644:Tartrates; C029768:tartaric acid