Determination of parthenolide in selected feverfew products by liquid chromatography.
The migraine prophylactic herb feverfew (Tanacetum parthenium L.) is marketed in the United States in a variety of forms and compositions. Although its therapeutic efficacy is still uncertain, the sesquiterpene lactone parthenolide is the constituent recommended to be measured for quality control of feverfew preparations. A validated liquid chromatographic method was developed and used to estimate parthenolide in a number of U.S. feverfew market products formulated as capsules, tablets, or crude powder. The method uses a Lichrosphere 5 C18 column, a mobile phase consisting of 50 mM NaH2PO4 in H2O (solvent A), and CH3CN-MeOH (90 + 10, v/v; solvent B). Elution was run at a flow rate of 1.0 mL/min with a linear gradient of 50-15% A in B over 20 min and UV detection at 210 nm. The correlation coefficient for the calibration curve was 0.9999 over the range of 0.00-0.400 mg/mL. Overall recovery of parthenolide was 103.1%.
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University of Mississippi, School of Pharmacy, National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, and Department of Pharmacognosy, University 38677, USA.
Khan
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Abourashed
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Khan
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D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D016527:Drug Costs; D004348:Drug Labeling; D006801:Humans; D016014:Linear Models; D019684:Magnoliopsida / Q000737:chemistry*; D008881:Migraine Disorders / Q000188:drug therapy; D004364:Pharmaceutical Preparations / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D012680:Sensitivity and Specificity; D012717:Sesquiterpenes / Q000032:analysis*; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004364:Pharmaceutical Preparations; D012717:Sesquiterpenes; D012996:Solutions; C002669:parthenolide