[Effect of chlorpromazine on the Ca2+-transport system of secretory cell membrane in Chironomus plumosus L. larval salivary glands].
Effect of chlorpromasine (specific blocking agent of calmoduline) on Na(+)-Ca(2+)-exchanger functioning, Ca(2+)-pump and potential dependent Ca(2+)-channels in plasmatic membrane of isolated salivary glands in Chironomus plumosus L. larvae was investigated. Addition of chlorpromasine in different concentrations to the incubation medium with physiological Na+ and K+ concentration increased Ca2+ content in the gland tissue and secretion of general protein by gland cells. Chlorpromasine addition to the hyposodium and hyperpotassium mediums decreased Ca2+ content in the gland tissue and protein secretion. We made a conclusion that chlorpromasine, as an inhibitor of calmoduline, blocks Na(+)-Ca(2+)-exchanger and Ca(2+)-pump of plasmatic membrane of secretory cells. Potentialdependent Ca(2+)-channels are also effectively blocked by chlorpromasine but mechanism of this process is unknown. We suppose that Ca(2+)-calmoduline complex forming leads to increase of calcium oscillations amplitude in the cells of the investigated glands and stimulation of secretion.
Man'ko
V V
VV
Ivan Franko National University of Lviv, Ukraine.
Klevets'
M Iu
MIu
Fedirko
N V
NV
Korol'
T V
TV
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000187:drug effects / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects; D002683:Chironomidae / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D002746:Chlorpromazine / Q000494:pharmacology*; D003470:Culture Media; D007814:Larva / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D012469:Salivary Glands / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D019831:Sodium-Calcium Exchanger / Q000187:drug effects / Q000378:metabolism
D015220:Calcium Channels; D003470:Culture Media; D019831:Sodium-Calcium Exchanger; D000252:Calcium-Transporting ATPases; D002118:Calcium; D002746:Chlorpromazine