Activation of murine macrophage-like cells by granulocytin.
Granulocytin, a C-type lectin from Sarcophaga peregrina (flesh fly), stimulated glucose consumption and cytokine production by the mouse macrophage-like cell line J774.1. When J774.1 cells were pretreated with tunicamycin, their granulocytin-dependent TNF-alpha production was greatly reduced. These results suggest that the stimulus of granulocytin is transmitted to J774.1 cells via the carbohydrate chain of granulocytin receptors located on their surface.
Fujita
Y
Y
Graduate School of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, Tokyo, 113-0033, Japan.
Homma
K J
KJ
Natori
S
S
D000818:Animals; D002460:Cell Line; D004175:Diptera / Q000737:chemistry*; D005947:Glucose / Q000378:metabolism; D006031:Glycosylation / Q000187:drug effects; D019476:Insect Proteins / Q000494:pharmacology; D015850:Interleukin-6 / Q000378:metabolism; D037102:Lectins / Q000494:pharmacology*; D037181:Lectins, C-Type; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism*; D051379:Mice; D011956:Receptors, Cell Surface / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014415:Tunicamycin / Q000494:pharmacology
C043030:20kDa lectin protein, Sarcophaga; D019476:Insect Proteins; D015850:Interleukin-6; D037102:Lectins; D037181:Lectins, C-Type; D011956:Receptors, Cell Surface; D014409:Tumor Necrosis Factor-alpha; D014415:Tunicamycin; D005947:Glucose