Cloning of PC3B, a novel member of the PC3/BTG/TOB family of growth inhibitory genes, highly expressed in the olfactory epithelium.
We identified in the EST database murine and human sequences similar, but not identical, to the members of the PC3/BTG/TOB family of cell cycle inhibitors. A conserved domain (aa 50-68) of the PC3 protein, the prototype member of the family, was used as a query. That domain has been shown by us to be necessary for the antiproliferative activity of PC3. A murine EST clone and a highly homologous human EST clone, containing the entire ORF, were chosen for sequencing. Comparison to databases and a phylogenetic tree analysis indicated that these EST clones are the mouse and human homologues of a gene that represents a novel member of the PC3/BTG/TOB family. This gene, named PC3B, is endowed with marked antiproliferative activity, being able to induce G(1) arrest, and is highly expressed in testis, in oocyte, and in preimplantation embryos. Analysis of its expression during murine development indicated a specific localization in the olfactory epithelium at midgestation, suggesting that PC3B might be involved in the differentiation of this neuronal structure. Human PC3B mapped to chromosome 11q23, as indicated by radiation hybrid analysis.
Buanne
P
P
Istituto di Neurobiologia, Istituto di Tecnologie Biomediche, CNR, Viale Marx 43, Rome, 00137, Italy.
Corrente
G
G
Micheli
L
L
Palena
A
A
Lavia
P
P
Spadafora
C
C
Lakshmana
M K
MK
Rinaldi
A
A
Banfi
S
S
Quarto
M
M
Bulfone
A
A
Tirone
F
F
D000595:Amino Acid Sequence; D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D002453:Cell Cycle / Q000235:genetics; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D019143:Evolution, Molecular; D020224:Expressed Sequence Tags; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D009831:Olfactory Mucosa / Q000166:cytology / Q000378:metabolism*; D016366:Open Reading Frames; D010802:Phylogeny; D043484:Proprotein Convertases; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
C415579:BTG4 protein, human; C485928:Btg4 protein, mouse; D018797:Cell Cycle Proteins; D043484:Proprotein Convertases; D016282:Aspartic Acid Endopeptidases