title: Megavesicles implicated in the rapid transport of intracisternal aggregates across the Golgi stack.
abstract: Engineered protein aggregates ranging up to 400 nm in diameter were selectively deposited within the cis-most cisternae of the Golgi stack following a 15 degrees C block. These aggregates are much larger than the standard volume of Golgi vesicles, yet they are transported across the stack within 10 min after warming the cells to 20 degrees C. Serial sectioning reveals that during the peak of anterograde transport, about 20% of the aggregates were enclosed in topologically free "megavesicles" which appear to pinch off from the rims of the cisternae. These megavesicles can explain the rapid transport of aggregates without cisternal progression on this time scale.
authors.affiliation: Cellular Biochemistry & Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA.
authors.forename: A
authors.initials: A
authors.lastname: Volchuk
authors.forename: M
authors.initials: M
authors.lastname: Amherdt
authors.forename: M
authors.initials: M
authors.lastname: Ravazzola
authors.forename: B
authors.initials: B
authors.lastname: Brügger
authors.forename: V M
authors.initials: VM
authors.lastname: Rivera
authors.forename: T
authors.initials: T
authors.lastname: Clackson
authors.forename: A
authors.initials: A
authors.lastname: Perrelet
authors.forename: T H
authors.initials: TH
authors.lastname: Söllner
authors.forename: J E
authors.initials: JE
authors.lastname: Rothman
authors.forename: L
authors.initials: L
authors.lastname: Orci
mesh_terms: D001692:Biological Transport; D002451:Cell Compartmentation; D006056:Golgi Apparatus / Q000378:metabolism* / Q000648:ultrastructure; D049452:Green Fluorescent Proteins; D013006:Growth Hormone / Q000235:genetics / Q000378:metabolism; D006801:Humans; D020104:Immunophilins / Q000235:genetics / Q000378:metabolism; D007425:Intracellular Membranes / Q000378:metabolism* / Q000648:ultrastructure; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008867:Microtomy; D011994:Recombinant Proteins / Q000378:metabolism; D022021:Tacrolimus Binding Proteins; D013696:Temperature; D013997:Time Factors; D014407:Tumor Cells, Cultured
chemical_list: D008164:Luminescent Proteins; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins; D013006:Growth Hormone; D022021:Tacrolimus Binding Proteins; D020104:Immunophilins