Hepatocyte growth factor is a predominant chemoattractant for gingival epithelial cells produced by radicular cyst-derived fibroblast-like cells.
To clarify whether fibroblasts could be involved in the pathogenesis of periodontal pocket formation, the chemotactic activity of radicular cyst-derived fibroblast-like cell (RCF)-conditioned medium (RCF-CM) for gingival epithelial cells was examined using a modified Boyden chamber assay. RCF-CM possessed significant chemotactic activity, which was decreased markedly by treatment with anti-human hepatocyte growth factor (HGF) antibody. Furthermore, the chemotactic activity of RCF-CM was well correlated with HGF content. These results show that the RCF secrete an HGF-like factor, and suggest that such a factor derived from periodontal fibroblasts might play a role in epithelial apical migration in periodontitis.
Ohshima
M
M
Department of Biochemistry, Nihon University School of Dentistry, Tokyo, Japan.
Nishiyama
T
T
Yamazaki
Y
Y
Yokosuka
R
R
Maeno
M
M
Otsuka
K
K
D002460:Cell Line; D002465:Cell Movement / Q000502:physiology; D002630:Chemotactic Factors / Q000494:pharmacology / Q000502:physiology*; D017077:Culture Media, Conditioned; D015194:Diffusion Chambers, Culture; D004847:Epithelial Cells / Q000187:drug effects / Q000502:physiology*; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology / Q000502:physiology*; D005881:Gingiva / Q000166:cytology* / Q000187:drug effects; D017228:Hepatocyte Growth Factor / Q000494:pharmacology / Q000502:physiology*; D006801:Humans; D010514:Periodontal Pocket / Q000209:etiology / Q000473:pathology; D010518:Periodontitis / Q000209:etiology / Q000473:pathology; D011842:Radicular Cyst / Q000473:pathology*
D002630:Chemotactic Factors; D017077:Culture Media, Conditioned; D017228:Hepatocyte Growth Factor