Reduction of the oxidative injury to the rabbits with established atherosclerosis by protein bound polysaccharide from Coriolus vesicolor.
Recent evidence has emerged that macrophage glutathione (GSH) content and selenium dependent glutathione peroxidase (SeGSHPx) activity are inversely related to cell-mediated oxidation of LDL, and intervention means to enhance the macrophage GSH-SeGSHPx status may contribute to attenuation of the atherosclerotic process. Our previous works showed that protein bound polysaccharide (PSK) injected intraperitoneally could enhance SeGSHPx activity and mRNA content of mouse macrophages. The aim of the present study is to demonstrate whether PSK can reduce the oxidative injury to the established atherosclerotic rabbits. Using the established atherosclerotic rabbit model, we studied the effect of PSK on oxidatively modified LDL (Ox-LDL), lipoperoxide (LPO) cholesterol contents and SeGSHPx activities of plasma and tissues (aorta, heart and liver) in the established atherosclerotic rabbits. As compared with the control group, Ox-LDL, LPO and cholesterol contents were much lower; SeGSHPx activities and SeGSHPx/LPO ratios were much higher in plasma and tissues (aorta, heart and liver); and the lesion area of aortae was reduced in the PSK group. Through the increment of SeGSHPx activity in macrophages and aortae, PSK enhances their antioxidation potentiality and improves the antioxidant/prooxidant imbalance in them, and thus decreases Ox-LDL, LPO and cholesterol contents of plasma and tissues, and regresses lesion area of aortae in the established atherosclerotic rabbits.
Zhou
M
M
Research Laboratory of Free Radical Medicine, The First Military Medical University, Guangzhou, China.
Chen
Y
Y
Ouyang
Q
Q
Liu
S X
SX
Pang
Z J
ZJ
D000818:Animals; D001011:Aorta / Q000187:drug effects; D001161:Arteriosclerosis / Q000517:prevention & control*; D002784:Cholesterol / Q000378:metabolism; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D020072:Polyporales; D011134:Polysaccharides / Q000494:pharmacology*; D011485:Protein Binding; D011817:Rabbits
D004365:Drugs, Chinese Herbal; D008054:Lipid Peroxides; D011134:Polysaccharides; D002784:Cholesterol; D005979:Glutathione Peroxidase