Lipopolyamines incorporating the tetraamine spermine, bound to an alkyl chain, sequester bacterial lipopolysaccharide.
Lipopolyamines, with high affinity for calf thymus DNA in an ethidium bromide displacement assay, bind with high affinity to bacterial lipopolysaccharide and neutralise in vitro endotoxic activity as determined by Griess nitric oxide and TNF-alpha ELISA assays.
Blagbrough
I S
IS
Department of Pharmacy and Pharmacology, University of Bath, UK. prsisb@bath.ac.uk
Geall
A J
AJ
David
S A
SA
D000818:Animals; D002460:Cell Line; D008070:Lipopolysaccharides / Q000378:metabolism*; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D011073:Polyamines / Q000378:metabolism*; D013096:Spermine / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D008070:Lipopolysaccharides; D011073:Polyamines; D014409:Tumor Necrosis Factor-alpha; D013096:Spermine; D009569:Nitric Oxide