The effect of nitecapone on early graft function in experimental single lung transplantation.
Nitecapone is an antioxidant molecule which has been shown to protect the heart against ischemia-reperfusion injury. We investigated whether a similar effect could be detected on lung graft preservation in a porcine model of single lung transplantation. Donors received either nitecapone or placebo in a modified Euro-Collins pulmonary flush solution. After cold storage for 19 h the left lung was transplanted. Patients in the nitecapone group received a nitecapone infusion during the graft reperfusion. A right-side heart bypass was used to measure flow distribution and pulmonary vascular resistance (PVR) in the recipient's transplanted and native lungs, respectively. Pulmonary vein blood samples were analyzed for blood gases, free radical trapping capacity and diene conjugates. PVR was high in the transplanted lung, which received only 20% of the blood flow. Oxygen tension in the transplanted lung was low (2.3-26.7 kPa). Nitecapone treatment increased the plasma free radical trapping capacity threefold. In spite of this increase in antioxidative capacity nitecapone could not protect the lung against ischemia-reperfusion injury when pulmonary hemodynamics, gas exchange or plasma diene conjugates were used as measures of lung graft function.
Vainikka
T
T
Department of Thoracic and Cardiovascular Surgery, Helsinki University Central Hospital, Finland.
Heikkilä
L
L
Toivonen
H J
HJ
Rämö
J
J
Hyytinen
T A
TA
Mattila
S P
SP
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002396:Catechols / Q000494:pharmacology*; D004195:Disease Models, Animal; D006085:Graft Survival / Q000187:drug effects*; D006439:Hemodynamics / Q000187:drug effects; D007262:Infusions, Intravenous; D016040:Lung Transplantation; D010422:Pentanones / Q000494:pharmacology*; D011659:Pulmonary Gas Exchange / Q000187:drug effects; D012016:Reference Values; D015427:Reperfusion Injury / Q000517:prevention & control*; D012680:Sensitivity and Specificity; D013552:Swine
D000975:Antioxidants; D002396:Catechols; D010422:Pentanones; C057364:nitecapone