Total morphine stability in urine specimens stored under various conditions.
The stability of total morphine in urine stored under various conditions was studied using control and experimental specimens. Samples in the control group were prepared using drug-free urine spiked with morphine at three concentration levels (300, 1000, and 2500 ng/mL), each with the pH adjusted to 5.5, 6.5, and 7.5. Samples in the experimental group came from 20 alleged heroin addicts (provided by Taipei Municipal Psychiatric Hospital). Samples in both groups were divided into two categories--one with and one without the precipitate (formed at 0 degrees C) removed. Samples in each of these two categories were further divided into two sub-groups--one with and one without sodium azide (0.05%) added. Total morphine contents in these samples were first determined by gas chromatography-mass spectrometry prior to storage and at 6, 12, 18, and 24 months following storage at -20, 4, 25, and 35 degrees C. Effects of sample treatment (azide addition and precipitate removal), pH, and storage temperature and length were evaluated by examining the percentage of total morphine remaining at the four time intervals following the initial determination. Major findings were as follows: (1) total morphine decomposition was minimal when stored for 12 months at -20 degrees C, which is a common current practice; (2) samples with lower initial sample pH had slower total morphine decomposition rates; and (3) azide addition appeared to have no detectable effect, whereas precipitate removal appeared to marginally reduce the decomposition rate, especially for samples with lower pH.
Chang
B L
BL
Division of Drug Chemistry, National Laboratories of Foods and Drugs, Department of Health, Nankang, Taipei, Taiwan.
Huang
M K
MK
Tsai
Y Y
YY
D004355:Drug Stability; D004356:Drug Storage; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009020:Morphine / Q000652:urine*; D009294:Narcotics / Q000652:urine*; D013048:Specimen Handling / Q000379:methods*; D015813:Substance Abuse Detection / Q000379:methods*; D013997:Time Factors
D009294:Narcotics; D009020:Morphine