DC-SIGN; a related gene, DC-SIGNR; and CD23 form a cluster on 19p13.
DC-SIGN is a C-type lectin, expressed on a dendritic cell subset. It is able to bind ICAM3 and HIV gp120 in a calcium-dependent manner. Here we report the genomic organization of DC-SIGN and map it to chromosome 19p13 adjacent to the C-type lectin CD23 (FcepsilonRII). We also report a novel, closely linked gene, DC-SIGNR, which shows 73% identity to DC-SIGN at the nucleic acid level and a similar genomic organization. Proteins encoded by both genes have tracts of repeats of 23 aa, predicted to form a coiled coil neck region. They also possess motifs that are known to bind mannose in a calcium-dependent fashion. We show concomitant expression of the two genes in endometrium, placenta, and stimulated KG1 cells (phenotypically similar to monocyte-derived dendritic cells). The existence of a DC-SIGN-related gene calls for reinterpretation of the HIV data to consider possible DC-SIGN/DC-SIGNR hetero-oligomerization.
Soilleux
E J
EJ
Immunology, Department of Pathology, Cambridge University, United Kingdom. ejs17@mole.bio.cam.ac.uk
Barten
R
R
Trowsdale
J
J
D000595:Amino Acid Sequence; D015815:Cell Adhesion Molecules; D002460:Cell Line; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19 / Q000276:immunology*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism*; D005091:Exons; D015870:Gene Expression / Q000276:immunology; D008040:Genetic Linkage; D006801:Humans; D007438:Introns; D037102:Lectins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D037181:Lectins, C-Type; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000276:immunology*; D009928:Organ Specificity / Q000235:genetics / Q000276:immunology; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D017455:Receptors, IgE / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
D015815:Cell Adhesion Molecules; D018076:DNA, Complementary; D037102:Lectins; D037181:Lectins, C-Type; D011956:Receptors, Cell Surface; D017455:Receptors, IgE; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin