Macromolecular structure determination by cryo-electron microscopy.
Recent advances in transmission electron microscopy (EM) hardware, low-temperature methods and image-processing software have made cryo-EM an important complement to X-ray crystallography and NMR for macromolecular structure determination, particularly of large assemblies. This review provides a summary of the main advances and a survey of the capabilities of this approach.
Saibil
H R
HR
Department of Crystallography, Birkbeck College, Malet Street, London WC1E 7HX, England. h.saibil@mail.cryst.bbk.ac.uk
D020285:Cryoelectron Microscopy / Q000379:methods*; D018360:Crystallography, X-Ray; D005583:Fourier Analysis; D007091:Image Processing, Computer-Assisted; D008958:Models, Molecular; D008968:Molecular Conformation; D019906:Nuclear Magnetic Resonance, Biomolecular; D012984:Software