title: Contrasting effects of estrogen on transthyretin and vitellogenin expression in males of the marine fish, Sparus aurata.
abstract: A partial cDNA encoding for the C-terminus of vitellogenin (VTG) was cloned from liver of Sparus aurata male treated with 17beta-estradiol (E(2)). E(2) treatment of S. aurata males resulted in increased synthesis and secretion of VTG protein into the plasma, determined by a specific enzyme-linked immunosorbent assay (ELISA) in a time-dependent manner. While VTG mRNA was induced by E(2) treatment, transthyretin (TTR) mRNA levels were reduced. These data provide the first demonstration that estrogen exhibits contrasting effect on VTG and on TTR gene expression in teleosts.
authors.affiliation: National Institute of Oceanography, Israel Oceanographic and Limnological Research, Tel Shikmona, PO Box 8030, 31080, Haifa, Israel. bruria@ocean.org.il
authors.forename: B
authors.initials: B
authors.lastname: Funkenstein
authors.forename: C J
authors.initials: CJ
authors.lastname: Bowman
authors.forename: N D
authors.initials: ND
authors.lastname: Denslow
authors.forename: M
authors.initials: M
authors.lastname: Cardinali
authors.forename: O
authors.initials: O
authors.lastname: Carnevali
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D008099:Liver / Q000737:chemistry; D008297:Male; D008969:Molecular Sequence Data; D011228:Prealbumin / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D021541:Sea Bream / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D014819:Vitellogenins / Q000097:blood / Q000235:genetics / Q000378:metabolism*
chemical_list: D011228:Prealbumin; D012333:RNA, Messenger; D014819:Vitellogenins; D004958:Estradiol