Development of a model system for evaluation of human endothelial cell proliferation in vitro: possible clinical applications.
A system for evaluation of the ability of human blood serum to affect endothelial cell proliferation was developed and tested. The system based on incorporation of (3)H-thymidine into DNA was used to analyze the effects of hormone replacement therapy on endothelial repair and angiogenesis. Blood serum from 12 menopausal women less effectively activated endothelial proliferation compared to control donor serum. After 6-month hormone replacement therapy with Divina (a combination of estradiol and medroxyprogesterone), this index increased in seven female subjects (58.3%), but remained below the control level. The model proposed by us can be used in clinical practice and drug testing for evaluation of the influence of blood serum on vascular endothelium.
Koval'chuk
N V
NV
Department of Biochemistry, State Research Center of Preventive Medicine, Ministry of Health of the Russian Federation.
Petrichenko
I E
IE
Olfer'ev
A M
AM
Shchukina
G N
GN
Popkov
S A
SA
Metel'skaya
V A
VA
D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division / Q000187:drug effects; D004338:Drug Combinations; D004730:Endothelium, Vascular / Q000187:drug effects / Q000473:pathology*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D020249:Hormone Replacement Therapy; D006801:Humans; D008525:Medroxyprogesterone / Q000494:pharmacology*; D008593:Menopause
D004338:Drug Combinations; C087853:divina; D004958:Estradiol; D008525:Medroxyprogesterone