Effect of chain length of HAV-VP3 synthetic peptides on its interaction with biomembrane models.
Shorter analogues of a continuous epitope of hepatitis A virus, VP3(110-121) peptide, failed to react with convalescent sera, indicating the importance of the entire peptide in the epitope structure. To better understand the influence of the structural properties of this 12-mer peptide epitope on its biological activity, the interaction of smaller peptide analogues with phospholipid biomembrane models was investigated by a combination of spectroscopic and biophysical techniques. In this article we describe our findings concerning the surface activity and the interaction of peptides with simple mono- and bilayer membranes composed of a zwitterionic phospholipid (dipalmitoyl phosphatidylcholine, DPPC), an anionic phospholipid (dipalmitoyl phosphatidylglicerol, DPPG), or a DPPC/DPPG mixture. The results indicate that the net negative charge of the peptide is in some way responsible of the specific interactions between VP3(110-121) and membrane phospholipids, and necessary to induce beta-type conformations upon vesicle interaction.
Sospedra
P
P
Physicochemical Department, Faculty of Pharmacy, Av. Joan XXIII s/n, 08028 Barcelona, Spain.
Muñoz
M
M
García
M
M
Alsina
M A
MA
Mestres
C
C
Haro
I
I
D002213:Capsid / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D036022:Capsid Proteins; D006507:Hepatovirus / Q000737:chemistry; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism*; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism / Q000494:pharmacology; D011485:Protein Binding; D011487:Protein Conformation / Q000187:drug effects; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship
D036022:Capsid Proteins; D008051:Lipid Bilayers; D008081:Liposomes; D010446:Peptide Fragments; D010455:Peptides; D010743:Phospholipids; C109000:VP3 peptide (110-121), hepatitis A virus