Immunohistochemistry and microwave decalcification of human temporal bones.
Processing of human temporal bones is a long, expensive process and the resulting celloidin sections are difficult to use for immunohistochemistry. We tested the ability of immunohistochemical assays to work in human temporal bones that were decalcified using a microwave oven. Tissue was trimmed to an approximate cube (1.5-2 cm/side) containing only the cochlea and immersed in fresh EDTA with paraformaldehyde every 6 h. This sized block required 190-400 h to decalcify. The decalcified tissue was embedded in paraffin and sectioned. Sections were immunoassayed with anti-cytochrome c oxidase, anti-neurofilament or anti-peripherin. All three antibodies labeled the appropriate structures. This procedure may stimulate advancement in the understanding of human inner ear pathology.
Keithley
E M
EM
Department of Surgery, Division of Otolaryngology, Head and Neck Surgery, University of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093-0666, USA. ekeithly@ucsd.edu
Truong
T
T
Chandronait
B
B
Billings
P B
PB
D002118:Calcium / Q000037:antagonists & inhibitors* / Q000378:metabolism; D003051:Cochlea / Q000378:metabolism / Q000528:radiation effects; D003576:Electron Transport Complex IV / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000378:metabolism; D008562:Membrane Glycoproteins; D008872:Microwaves; D009419:Nerve Tissue Proteins / Q000378:metabolism; D016900:Neurofilament Proteins / Q000378:metabolism; D064531:Peripherins; D013701:Temporal Bone / Q000378:metabolism* / Q000528:radiation effects*
D007381:Intermediate Filament Proteins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D016900:Neurofilament Proteins; C578994:PRPH protein, human; D064531:Peripherins; D003576:Electron Transport Complex IV; D002118:Calcium