Mitotic phosphorylation of histone H3 is governed by Ipl1/aurora kinase and Glc7/PP1 phosphatase in budding yeast and nematodes.
Phosphorylation of histone H3 at serine 10 occurs during mitosis and meiosis in a wide range of eukaryotes and has been shown to be required for proper chromosome transmission in Tetrahymena. Here we report that Ipl1/aurora kinase and its genetically interacting phosphatase, Glc7/PP1, are responsible for the balance of H3 phosphorylation during mitosis in Saccharomyces cerevisiae and Caenorhabditis elegans. In these models, both enzymes are required for H3 phosphorylation and chromosome segregation, although a causal link between the two processes has not been demonstrated. Deregulation of human aurora kinases has been implicated in oncogenesis as a consequence of chromosome missegregation. Our findings reveal an enzyme system that regulates chromosome dynamics and controls histone phosphorylation that is conserved among diverse eukaryotes.
Hsu
J Y
JY
Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville 22908, USA.
Sun
Z W
ZW
Li
X
X
Reuben
M
M
Tatchell
K
K
Bishop
D K
DK
Grushcow
J M
JM
Brame
C J
CJ
Caldwell
J A
JA
Hunt
D F
DF
Lin
R
R
Smith
M M
MM
Allis
C D
CD
D000818:Animals; D064127:Aurora Kinases; D017173:Caenorhabditis elegans / Q000166:cytology* / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism*; D016678:Genome; D015801:Helminth Proteins / Q000378:metabolism; D006657:Histones / Q000378:metabolism*; D008938:Mitosis; D010641:Phenotype; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D016372:RNA, Antisense; D034741:RNA, Small Interfering; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000378:metabolism; D012694:Serine / Q000378:metabolism; D013045:Species Specificity
D005656:Fungal Proteins; D015801:Helminth Proteins; D006657:Histones; D016372:RNA, Antisense; D034741:RNA, Small Interfering; D012694:Serine; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases; D010749:Phosphoprotein Phosphatases