title: Invasion of strongly binding oligonucleotides into tRNA structure.
abstract: Interaction of yeast tRNA(Phe) with oligodeoxyribonucleotides containing 5-methylcytosine, 2-aminoadenine, and 5-propynyl-2'-deoxyuridine was investigated. The modified oligonucleotides show increased binding capacity although the association rates are similar for the modified and natural oligonucleotides. The most pronounced increase in association constant (70 times) due to the incorporation of the strongly binding units was achieved in the case of oligonucleotide complementary to the sequence 65-76 of the tRNA(Phe).
authors.affiliation: Laboratory of Nucleic Acids Biochemistry, Novosibirsk Institute of Bioorganic Chemistry, Russia.
authors.forename: V
authors.initials: V
authors.lastname: Petyuk
authors.forename: R
authors.initials: R
authors.lastname: Serikov
authors.forename: V
authors.initials: V
authors.lastname: Tolstikov
authors.forename: V
authors.initials: V
authors.lastname: Potapov
authors.forename: R
authors.initials: R
authors.lastname: Giege
authors.forename: M
authors.initials: M
authors.lastname: Zenkova
authors.forename: V
authors.initials: V
authors.lastname: Vlassov
mesh_terms: D015075:2-Aminopurine / Q000031:analogs & derivatives* / Q000737:chemistry; D044503:5-Methylcytosine; D003596:Cytosine / Q000031:analogs & derivatives / Q000737:chemistry; D003857:Deoxyuridine / Q000031:analogs & derivatives* / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D009841:Oligonucleotides / Q000378:metabolism*; D012360:RNA, Transfer, Phe / Q000737:chemistry* / Q000378:metabolism; D013997:Time Factors; D015003:Yeasts / Q000737:chemistry
chemical_list: D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D012360:RNA, Transfer, Phe; D015075:2-Aminopurine; C007300:2,6-diaminopurine; D044503:5-Methylcytosine; C030125:5-(2-propenyl)-2'-deoxyuridine; D003596:Cytosine; D003857:Deoxyuridine