A highly sensitive and specific enzyme-linked immunosorbent assay of antibodies to hepatitis C virus.
In this study, a 178 amino acids long portion of the hepatitis C virus (HCV) core gene was cloned, sequenced, expressed in Escherichia coli, and purified. The resulting antigen (C178) was tested with human sera enzyme-linked immunosorbent assay (ELISA) in order to assess its ability to diagnose HCV. It was shown by ELISA that 92% of the patients sera, diagnosed previously by a 3rd generation enzyme immunoassay (EIA) as HCV-positive, had antibodies against the C178 antigen. This antigen gave no false positive results when tested with anti-HCV-negative sera.
Eroglu
C
C
Department of Molecular Biology and Genetics, Science Faculty, Bilkent University, Ankara, Turkey.
Yildiz
E
E
Ozturk
M
M
Pinarbasi
E
E
D003001:Cloning, Molecular; D004279:DNA, Viral / Q000096:biosynthesis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics; D016174:Hepacivirus / Q000276:immunology; D006526:Hepatitis C / Q000821:virology*; D018937:Hepatitis C Antibodies / Q000097:blood*; D018936:Hepatitis C Antigens / Q000096:biosynthesis / Q000276:immunology*; D006801:Humans; D011994:Recombinant Proteins / Q000096:biosynthesis; D012680:Sensitivity and Specificity; D012698:Serologic Tests; D014758:Viral Core Proteins / Q000096:biosynthesis / Q000235:genetics
D004279:DNA, Viral; D018937:Hepatitis C Antibodies; D018936:Hepatitis C Antigens; D011994:Recombinant Proteins; D014758:Viral Core Proteins