Synthesis of 60- and 72 kDa heat shock proteins in early porcine embryogenesis.
Proteins of selected embryonic stages were metabolically labeled with [(35)S]-methionine and analyzed by two-dimensional SDS-polyacrylamide gel electrophoresis (2-D PAGE) to study protein expression from 4- to 8-cell to blastocyst stage of porcine embryos. Two proteins with molecular weights of 60 and 72kDa were de novo synthesized during the 4- to 8-cell stage were the earliest that were detected. They were identified as HSP60 and HSP72 according to their locations on 2-D autoradiography and the immunoblotting result of anti-HSP 60 and HSP 72 antibodies of 1-cell stage of porcine embryos. In protein translation in early pig embryogenesis the timing of their synthesis suggests that HSP60 and HSP72 play significant roles as chaperones.
King
Y T
YT
Department of Comparative Medicine, Pig Research Institute Taiwan, P. O. Box 23, Chunan, 35099, ROC, Miaoli, Taiwan.
Lee
W C
WC
Gao
M S
MS
Wang
J L
JL
Tu
C F
CF
Wu
S C
SC
Kuo
Y H
YH
D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D018834:Chaperonin 60 / Q000096:biosynthesis*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000662:veterinary; D005260:Female; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D007315:Insemination, Artificial / Q000662:veterinary; D008297:Male; D008970:Molecular Weight; D013552:Swine / Q000196:embryology*
D018834:Chaperonin 60; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins