Analogies between halorhodopsin and bacteriorhodopsin.
The light-activated proton-pumping bacteriorhodopsin and chloride ion-pumping halorhodopsin are compared. They belong to the family of retinal proteins, with 25% amino acid sequence homology. Both proteins have seven alpha helices across the membrane, surrounding the retinal binding pocket. Photoexcitation of all-trans retinal leads to ion transporting photocycles, which exhibit great similarities in the two proteins, despite the differences in the ion transported. The spectra of the K, L, N and O intermediates, calculated using time-resolved spectroscopic measurements, are very similar in both proteins. The absorption kinetic measurements reveal that the chloride ion transporting photocycle of halorhodopsin does not have intermediate M characteristic for deprotonated Schiff base, and intermediate L dominates the process. Energetically the photocycle of bacteriorhodopsin is driven mostly by the decrease of the entropic energy, while the photocycle of halorhodopsin is enthalpy-driven. The ion transporting steps were characterized by the electrogenicity of the intermediates, calculated from the photoinduced transient electric signal measurements. The function of both proteins could be described with the 'local access' model developed for bacteriorhodopsin. In the framework of this model it is easy to understand how bacteriorhodopsin can be converted into a chloride pump, and halorhodopsin into a proton pump, by changing the ion specificity with added ions or site-directed mutagenesis.
Váró
G
G
Institute of Biophysics, Biological Research Center of the Hungarian Academy of Sciences, H-6701, Szeged, Hungary.
D000595:Amino Acid Sequence; D001436:Bacteriorhodopsins / Q000737:chemistry*; D002712:Chlorides / Q000737:chemistry; D025602:Halorhodopsins; D016623:Ion Pumps / Q000737:chemistry*; D008969:Molecular Sequence Data; D010777:Photochemistry; D011487:Protein Conformation; D017494:Proton Pumps / Q000737:chemistry*; D012545:Schiff Bases / Q000737:chemistry; D016415:Sequence Alignment
D002712:Chlorides; D025602:Halorhodopsins; D016623:Ion Pumps; D017494:Proton Pumps; D012545:Schiff Bases; D001436:Bacteriorhodopsins