title: Sensitive high-performance thin-layer chromatographic method for the estimation of diospyrin, a tumour inhibitory agent from the stem bark of Diospyros montana Roxb.
abstract: Diospyrin, a tumour inhibitory agent from the stem bark of Diospyros montana was isolated and characterised. A sensitive high-performance thin-layer chromatographic (HPTLC) method was developed for the estimation of diospyrin. The method was validated for precision (intra- and inter-day), repeatability and accuracy. The method was found to be precise, with the RSDs for intra-day in the range of 0.72-1.85% and RSDs for inter-day in the range of 1.06-2.95%, for different concentrations. Instrumental precision and repeatability of the method were found to be 0.086 and 0.937 (% CV), respectively. Accuracy of the method was checked by performing the recovery study at two levels and average percentage recovery was found to be 97.87%. The developed HPTLC method was adopted for the estimation of diospyrin content of the stem bark of D. montana from different regions, which varied from 0.35 to 0.47% (w/w) in the samples.
authors.affiliation: BV Patel Pharmaceutical Education and Research Development Centre, Thaltej, Ahmedabad, India.
authors.forename: M N
authors.initials: MN
authors.lastname: Ravishankara
authors.forename: N
authors.initials: N
authors.lastname: Shrivastava
authors.forename: M G
authors.initials: MG
authors.lastname: Jayathirtha
authors.forename: H
authors.initials: H
authors.lastname: Padh
authors.forename: M
authors.initials: M
authors.lastname: Rajani
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis*; D002855:Chromatography, Thin Layer / Q000379:methods*; D019684:Magnoliopsida / Q000737:chemistry*; D009285:Naphthoquinones / Q000032:analysis*; D018547:Plant Stems / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D009285:Naphthoquinones; C043546:diospyrin