Human AICAR transformylase: role of the 4-carboxamide of AICAR in binding and catalysis.
We have prepared 4-substituted analogues of 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) to investigate the specificity and mechanism of AICAR transformylase (AICAR Tfase). Of the nine analogues of AICAR studied, only one analogue, 5-aminoimidazole-4-thiocarboxamide ribonucleotide, was a substrate, and it was converted to 6-mercaptopurine ribonucleotide. The other analogues either did not bind or were competitive inhibitors, the most potent being 5-amino-4-nitroimidazole ribonucleotide with a K(i) of 0.7 +/- 0.5 microM. The results show that the 4-carboxamide of AICAR is essential for catalysis, and it is proposed to assist in mediating proton transfer, catalyzing the reaction by trapping of the addition compound. AICAR analogues where the nitrogen of the 4-carboxamide was derivatized with a methyl or an allylic group did not bind AICAR Tfase, as determined by pre-steady-state burst kinetics; however, these compounds were potent inhibitors of IMP cyclohydrolase (IMP CHase), a second activity of the bifunctional mammalian enzyme (K(i) = 0.05 +/- 0.02 microM for 4-N-allyl-AlCAR). It is proposed that the conformation of the carboxamide moiety required for binding to AICAR Tfase is different than the conformation required for binding to IMP CHase, which is supported by inhibition studies of purine ribonucleotides. It is shown that 5-formyl-AICAR (FAICAR) is a product inhibitor of AICAR Tfase with K(i) of 0.4 +/- 0.1 microM. We have determined the equilibrium constant of the transformylase reaction to be 0.024 +/- 0.001, showing that the reaction strongly favors AICAR and the 10-formyl-folate cofactor. The coupling of the AICAR Tfase and IMP CHase activities on a single polypeptide allows the overall conversion of AICAR to IMP to be favorable by coupling the unfavorable formation of FAICAR with the highly favorable cyclization reaction. The current kinetic studies have also indicated that the release of FAICAR is the rate-limiting step, under steady-state conditions, in the bifunctional enzyme and channeling is not observed between AICAR Tfase and IMP CHase.
Wall
M
M
Department of Chemistry, 415 Wartik Laboratory, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.
Shim
J H
JH
Benkovic
S J
SJ
D000249:Adenosine Monophosphate / Q000737:chemistry; D000577:Amides / Q000737:chemistry; D000620:Aminoimidazole Carboxamide / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000737:chemistry; D001665:Binding Sites; D001692:Biological Transport; D002384:Catalysis; D004791:Enzyme Inhibitors / Q000737:chemistry; D006801:Humans; D019877:Hydroxymethyl and Formyl Transferases / Q000037:antagonists & inhibitors / Q000737:chemistry*; D007700:Kinetics; D009097:Multienzyme Complexes / Q000037:antagonists & inhibitors / Q000737:chemistry; D009709:Nucleotide Deaminases / Q000037:antagonists & inhibitors / Q000737:chemistry; D051027:Phosphoribosylaminoimidazolecarboxamide Formyltransferase; D011487:Protein Conformation; D012263:Ribonucleosides / Q000737:chemistry; D012265:Ribonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D019820:Xanthine
D000577:Amides; D004791:Enzyme Inhibitors; D009097:Multienzyme Complexes; D012263:Ribonucleosides; D012265:Ribonucleotides; C062030:5-formamidoimidazole-4-carboxamide ribotide; D019820:Xanthine; D000620:Aminoimidazole Carboxamide; D000249:Adenosine Monophosphate; C011141:xanthosine monophosphate; D019877:Hydroxymethyl and Formyl Transferases; D051027:Phosphoribosylaminoimidazolecarboxamide Formyltransferase; D009709:Nucleotide Deaminases; C021436:IMP cyclohydrolase; C031143:AICA ribonucleotide