Determination of glucosinolates in rapeseed and Thlaspi caerulescens plants by liquid chromatography-atmospheric pressure chemical ionization mass spectrometry.
Liquid chromatography-atmospheric pressure chemical ionization mass spectrometry was used to identify glucosinolates in plant extracts. Optimization of the analytical conditions and the determination of the method detection limit was performed using commercial 2-propenylglucosinolate (sinigrin). Optimal values for the following parameters were determined: nebulization pressure, gas temperature, flux of drying gas, capillar voltage, corona current and fragmentor conditions. The method detection limit for sinigrin was 2.85 ng. For validation of the method the glucosinolates in reference material (rapeseed) from the Community Bureau of Reference Materials (BCR) were analyzed. The method was applied for the determination of glucosinolates in Thlaspi caerulescens plants.
Tolrà
R P
RP
Laboratorio de Fisiología Vegetal, Facultad de Ciencias, Universidad Autónoma de Barcelona, Bellaterra, Spain.
Alonso
R
R
Poschenrieder
C
C
Barceló
D
D
Barceló
J
J
D001274:Atmospheric Pressure; D001937:Brassica / Q000737:chemistry*; D019607:Brassicaceae / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D005961:Glucosinolates / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D012015:Reference Standards
D005961:Glucosinolates; D010936:Plant Extracts