Beta agonist regulation of sodium transport in fetal lung epithelium: roles of cell volume, cytosolic chloride and protein tyrosine kinase.
1) A beta agonist stimulated Na+ transport and decreased the intracellular Cl concentration ([Cl]c) associated with cell shrinkage via an increase in cytosolic cAMP level by activating adenylate cyclase in rat fetal distal lung epithelial (FDLE) cells. 2) Lowering [Cl-]c activated a 28-pS nonselective cation (NSC) channel by elongating the open time of the channel. 3) cAMP signals were converted to a protein tyrosine kinase (PTK)-mediated signal. 4) The PTK-mediated signal was involved in the cAMP-stimulated Na+ transport in rat FDLE cells.
Marunaka
Y
Y
Department of Physiology, Kyoto Prefectural University of Medicine, Japan. marunaka@phys.kpu-m.ac.jp
Niisato
N
N
Ito
Y
Y
D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects / Q000502:physiology; D048429:Cell Size / Q000502:physiology; D002712:Chlorides / Q000378:metabolism*; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D005333:Fetus / Q000166:cytology; D009579:Nitrobenzoates / Q000494:pharmacology; D011247:Pregnancy; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D020545:Respiratory Mucosa / Q000166:cytology / Q000196:embryology / Q000201:enzymology*; D012964:Sodium / Q000378:metabolism*; D020032:Tyrphostins / Q000494:pharmacology
D000318:Adrenergic beta-Agonists; D002712:Chlorides; D004791:Enzyme Inhibitors; D009579:Nitrobenzoates; D020032:Tyrphostins; D005576:Colforsin; C058176:5-nitro-2-(3-phenylpropylamino)benzoic acid; D012964:Sodium; D000242:Cyclic AMP; D011505:Protein-Tyrosine Kinases; C060641:tyrphostin A23