Cloning and localization of C2orf2(ropp120), a previously unknown WD repeat protein.
Ropp 120 (restrictedly overexpressed proliferation-associated protein) is a cytoplasmic protein of 120 kDa that is significantly overexpressed in mitotic cells. Protein sequencing of the immunoaffinity purified 120-kDa protein showed it to be an as yet unknown protein. DNA sequencing revealed a cDNA sequence of 3419 bases, which includes the complete coding region of ropp120 of 2943 bases (981 amino acids). Analysis of the deduced amino acid sequence showed that ropp 120 contains four WD repeats and a well-conserved consensus sequence of serine proteases. The gene encoding ropp120 (HGMW-approved gene symbol C2orf2) was assigned to chromosome 2p21-p22 by means of radiation hybrid and fluorescence in situ hybridization mapping.
Heidebrecht
H J
HJ
Department of Hematopathology and Lymph Node Registry, German Association of Pathologists, University of Kiel, Michaelisstrasse 11, Kiel, D-24105, Germany. hheidebrecht@path.uni-kiel.de
Buck
F
F
Pollmann
M
M
Siebert
R
R
Parwaresch
R
R
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002453:Cell Cycle / Q000235:genetics*; D018797:Cell Cycle Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000378:metabolism; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007739:L Cells; D051379:Mice; D008869:Microtubule-Associated Proteins; D008938:Mitosis / Q000235:genetics*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D020449:Repetitive Sequences, Amino Acid; D016324:Sequence Tagged Sites; D012697:Serine Endopeptidases / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*
D018797:Cell Cycle Proteins; D008869:Microtubule-Associated Proteins; D011994:Recombinant Proteins; C415585:EML4 protein, human; D012697:Serine Endopeptidases