Equivalence of Visual Immunoprecipitate Assay (VIP) for Salmonella for the detection of motile and nonmotile Salmonella in all foods to AOAC culture method: collaborative study.
Six foods representative of a wide variety of processed, dried powder processed, and raw food types were analyzed by the Visual Immunoprecipitate Assay (VIP) for Salmonella and AOAC INTERNATIONAL culture method. Paired samples of each food type were simultaneously analyzed; one sample by the VIP method and one by the AOAC culture method. A total of 24 laboratories representing federal government agencies and private industry, in the United States and Canada, participated in this collaborative study. Food types were inoculated with species of Salmonella with the exception of raw ground chicken, which was naturally contaminated. No statistical differences (p < 0.05) were observed between VIP for Salmonella interpretation and the AOAC culture method for any inoculation level of any food type or naturally contaminated food. The method was adopted Official First Action status by AOAC INTERNATIONAL.
Feldsine
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BioControl Systems, Inc., Bellevue, WA 98005, USA.
Mui
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Forgey
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Kerr
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D000818:Animals; D002099:Cacao / Q000382:microbiology; D002170:Canada; D002645:Chickens; D004531:Eggs / Q000382:microbiology; D005516:Food Microbiology; D006077:Government Agencies; D007054:Ice Cream / Q000382:microbiology; D007163:Immunosorbent Techniques; D007753:Laboratories; D008460:Meat / Q000382:microbiology; D008892:Milk / Q000382:microbiology; D011202:Poultry Products / Q000382:microbiology; D011786:Quality Control; D012475:Salmonella / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013552:Swine; D014481:United States