title: Cloning and sequencing analysis of Trp1 gene of Flammulina velutipes.
abstract: The genomic TRP1 gene from basidiomycete Flammulina velutipes was cloned by complementation of yeast Saccharomyces cerevisiae trp1 mutation. Sequencing analysis revealed that the TRP1 gene encoded a single protein consisting of three catalytic functional domains; glutamine amidotransferase, indole-3-glycerol phosphate synthase ) and N-(5'-phosphoribosyl) anthranilate isomerase, in order of NH2-glutamine amidotransferase-indole-3-glycerol phosphate synthase N-(5'-phosphoribosyl) anthranilate isomerase-COOH. The coding sequence of the TRP1 gene was interrupted by a single intron of 48 bases, the position and flanking sequences of which were highly homologous to those of basidiomycete Phanerochaete chrysosporium trpC.
authors.affiliation: Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University, Nagano, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Nakai
authors.forename: K
authors.initials: K
authors.lastname: Sen
authors.forename: S
authors.initials: S
authors.lastname: Kurosawa
authors.forename: H
authors.initials: H
authors.lastname: Shibai
mesh_terms: D000363:Agaricales / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D019747:Aldose-Ketose Isomerases; D000595:Amino Acid Sequence; D000878:Anthranilate Synthase / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005822:Genetic Vectors; D007438:Introns; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D029701:Saccharomyces cerevisiae Proteins; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014170:Transformation, Genetic
chemical_list: D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; D000878:Anthranilate Synthase; D019747:Aldose-Ketose Isomerases; C490353:TRP1 protein, S cerevisiae