title: Corneal graft endothelial cell densities after preservation in M-K, K-sol and MEM tissue culture media. A clinical and specular microscopic study.
abstract: The endothelial cell densities of corneal transplants of 30 keratoconus patients have been studied. All operations were performed by the senior author using the same technique. Preservation systems M-K medium, MEM-solution and K-sol were used; ten grafts in each group. The average endothelial cell density for all 30 transplants was 1815 +/- 869 cells/mm2. The mean cell density was the lowest (1128 +/- 587 cells/mm2) and the mean follow-up time longest (2 years and 9 months) in M-K medium stored grafts. K-sol is the newest preservation method used in our hospital. It gave the best graft endothelial cell densities (2302 +/- 938 cells/mm2). The mean follow-up time was shortest in K-sol (8 months). The mean endothelial cell density for grafts from MEM-solution was 2015 +/- 614 cells/mm2) with the mean follow-up time of 1 year, 4 months.
authors.affiliation: Department of Ophthalmology, University of Helsinki, Finland.
authors.forename: P
authors.initials: P
authors.lastname: Ruusuvaara
authors.forename: K
authors.initials: K
authors.lastname: Setälä
mesh_terms: D000293:Adolescent; D000328:Adult; D002452:Cell Count / Q000187:drug effects; D002809:Chondroitin Sulfates; D016039:Corneal Transplantation; D003470:Culture Media / Q000494:pharmacology*; D004728:Endothelium, Corneal / Q000187:drug effects / Q000473:pathology*; D006531:HEPES; D006801:Humans; D008875:Middle Aged; D009930:Organic Chemicals; D011309:Preservation, Biological
chemical_list: D003470:Culture Media; C051174:K-Sol preserving medium; C015368:McCarey-Kaufman medium; D009930:Organic Chemicals; D002809:Chondroitin Sulfates; D006531:HEPES