[Studies on transgenic oilseed rape (Brassica napus) plants transformed with beta-1,3-glucanase and chitinase genes and its resistance to Sclerotinia sclerotiorium].
By Agrobacterium-mediated method, the cotyledonary petiole of good quality rape variety H165 was transformed with plant expression vector pBLGC which constitutively express beta-1,3-glucanase and chitinase genes. We obtained some Kanamycin(Kan)-resistant regenerational green shoots, with these shoots, PCR identification was conducted. Results showed that 30% green shoots which grew in medium of Kan 15 mg/L and 53% green shoots in Kan 25 mg/L had positive reaction. We also made dot blot analysis with those green shoots, some of them gave positive signal, indicating that the foreign genes had integrated into rape genome. Fungal challenge of these transgenic plants showed that some plants were much more resistant to Sclerotinia sclerotiorium than non-transgenic control plants.
Lan
H Y
HY
Institute of Genetics, Chinese Academy of Sciences, Beijing.
Wang
C H
CH
Zhang
L H
LH
Liu
G Z
GZ
Wan
L L
LL
Chen
Z H
ZH
Tian
Y C
YC
D001203:Ascomycota / Q000235:genetics*; D001937:Brassica / Q000235:genetics* / Q000382:microbiology*; D002688:Chitinases / Q000235:genetics*; D043326:Glucan 1,3-beta-Glucosidase; D030821:Plants, Genetically Modified; D014170:Transformation, Genetic; D001617:beta-Glucosidase / Q000235:genetics*
D002688:Chitinases; D001617:beta-Glucosidase; D043326:Glucan 1,3-beta-Glucosidase