Schizosaccharomyces pombe Rad9 contains a BH3-like region and interacts with the anti-apoptotic protein Bcl-2.
Here we report that the Schizosaccharomyces pombe Rad9 (SpRad9) protein contains a group of amino acids with similarity to the Bcl-2 homology 3 death domain, which is required for SpRad9 interaction with human Bcl-2 and apoptosis induction in human cells. Overexpression of Bcl-2 in S. pombe inhibits cell growth independently of rad9, but enhances resistance of rad9-null cells to methyl methanesulfonate, ultraviolet and ionizing radiation. These observations suggest that SpRad9 may represent the first member of the Bcl-2 protein family identified in yeast, though the cell death pathways in S. pombe may differ from those found in mammals.
Komatsu
K
K
Drug Discovery Program, H. Lee Moffitt Cancer Center, 12902 Magnolia Drive, Tampa, FL 33612, USA.
Hopkins
K M
KM
Lieberman
H B
HB
Wang
H
H
D017209:Apoptosis / Q000187:drug effects; D001665:Binding Sites; D018797:Cell Cycle Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D017124:Conserved Sequence / Q000235:genetics; D004249:DNA Damage / Q000187:drug effects / Q000235:genetics; D019143:Evolution, Molecular; D017353:Gene Deletion; D015870:Gene Expression; D016153:Genes, Suppressor / Q000235:genetics; D006801:Humans; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D009153:Mutagens / Q000494:pharmacology; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000166:cytology / Q000187:drug effects / Q000235:genetics; D013831:Thiamine / Q000494:pharmacology; D020798:Two-Hybrid System Techniques
D018797:Cell Cycle Proteins; D009153:Mutagens; D019253:Proto-Oncogene Proteins c-bcl-2; C069930:rad9 protein; D008741:Methyl Methanesulfonate; D013831:Thiamine