Flow cytometric measurement of intracellular cytokines.
The identification of distinct T helper lymphocyte subsets (Th1/2) with polarised cytokine production has opened up new fields in immunobiology. Of the several alternative methods of monitoring cytokine production, flow cytometric analysis of intracellular staining has distinct advantages and pitfalls. It allows high throughput of samples and multiparameter characterisation of cytokine production on a single cell basis without the need for prolonged in vitro culture and cloning. However, these methods may cause important changes in cell surface phenotype which can make interpretation difficult.
Pala
P
P
Department of Respiratory Medicine, National Heart and Lung Institute, Imperial College of Science, Technology and Medicine, W2 1PG, London, UK. p.pala@ic.ac.uk
Hussell
T
T
Openshaw
P J
PJ
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D001491:Basophils / Q000378:metabolism; D015704:CD4 Antigens / Q000032:analysis; D016207:Cytokines / Q000032:analysis*; D015536:Down-Regulation; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007371:Interferon-gamma / Q000032:analysis; D016753:Interleukin-10 / Q000032:analysis; D015847:Interleukin-4 / Q000032:analysis; D007700:Kinetics; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D051379:Mice; D011786:Quality Control; D018417:Th1 Cells / Q000378:metabolism; D018418:Th2 Cells / Q000378:metabolism
D000954:Antigens, Surface; D015704:CD4 Antigens; D016207:Cytokines; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma