Molecular cloning and characterization of the Fugu rubripes MEST/COPG2 imprinting cluster and chromosomal localization in Fugu and Tetraodon nigroviridis.
We isolated Fugu genomic clones using the human MEST (Mesoderm-Specific Transcript) cDNA as probe. Sequence analysis revealed the presence of MEST and three additional genes which show homology to plant DNBP (DNA-Binding Protein), vertebrate COPG2 (Coat Protein Gamma 2), as well as to human and mouse UCN (Urocortin). Structures of Fugu and human MEST, COPG2 and UCN genes are very similar. Since MEST and COPG2 are neighboring genes on human chromosome 7q32, we can conclude that we identified their orthologs and that linkage of these genes is evolutionarily conserved in vertebrates. Unlike human MEST which underlies isoform-specific imprinting and is methylated in a parent-of-origin-specific fashion, the CpG island of the Fugu ortholog is completely methylated. The translation start of Fugu MEST is identical to the non-imprinted human isoform which is in good agreement with the assumption that genomic imprinting is restricted to mammals. Comparative mapping of these genes by fluorescence in-situ hybridization to metaphase chromosomes of Fugu rubripes and Tetraodon nigroviridis showed clear signals on one of the smallest acrocentric chromosomal pairs, which in Fugu, can be easily classified by its unique triangular shape.
Brunner
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B
Human Genetics, University Hospital Nijmegen, The Netherlands.
Grützner
F
F
Yaspo
M L
ML
Ropers
H H
HH
Haaf
T
T
Kalscheuer
V M
VM
D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D020756:Coatomer Protein; D003346:Corticotropin-Releasing Hormone / Q000235:genetics; D003360:Cosmids; D046508:Culture Techniques; D004268:DNA-Binding Proteins / Q000235:genetics; D005399:Fishes / Q000235:genetics*; D018392:Genomic Imprinting; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D011506:Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D054832:Urocortins; D033921:Vesicular Transport Proteins; D015027:Zebrafish
C485814:COPG2 protein, human; D020756:Coatomer Protein; C410034:Copg2 protein, mouse; D004268:DNA-Binding Proteins; D011506:Proteins; D054832:Urocortins; D033921:Vesicular Transport Proteins; C103637:mesoderm specific transcript protein; D003346:Corticotropin-Releasing Hormone