title: Lipopolysaccharide increases endogenous morphine levels in rat brain.
abstract: The present study was designed to determine whether whole body injection of lipopolysaccharide increases endogenous rat brain morphine levels. High performance liquid chromatography coupled to electrochemical detection and gas-chromatography mass spectrometry reveal that the resting brain morphine level is 7.0+/-3.2ng/g wet weight. In a time dependent manner, intraperitoneal injection of lipopolysaccharide (10 microg), leads to an increase of brain morphine level with a peak reaching after 36h post injection. After 72h following the lipopolysaccharide-injection, morphine levels increased to 66.0+/-5.4ng/g brain wet weight (P<0.001). Furthermore, 96h of fasting also increased the brain morphine level to 44.0+/-3. 6ng/g of brain wet weight. These results suggest that rat brain morphinergic processes respond to organismic challenges after a significant latent period.
authors.affiliation: Neuroscience Research Institute, State University of New York, College at Old Westbury, NY 11568, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Goumon
authors.forename: S
authors.initials: S
authors.lastname: Bouret
authors.forename: F
authors.initials: F
authors.lastname: Casares
authors.forename: W
authors.initials: W
authors.lastname: Zhu
authors.forename: J C
authors.initials: JC
authors.lastname: Beauvillain
authors.forename: G B
authors.initials: GB
authors.lastname: Stefano
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D007274:Injections, Intraperitoneal; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D009020:Morphine / Q000378:metabolism*; D051381:Rats
chemical_list: D008070:Lipopolysaccharides; D009020:Morphine