Alteration of stimulus-specific guard cell calcium oscillations and stomatal closing in Arabidopsis det3 mutant.
Cytosolic calcium oscillations control signaling in animal cells, whereas in plants their importance remains largely unknown. In wild-type Arabidopsis guard cells abscisic acid, oxidative stress, cold, and external calcium elicited cytosolic calcium oscillations of differing amplitudes and frequencies and induced stomatal closure. In guard cells of the V-ATPase mutant det3, external calcium and oxidative stress elicited prolonged calcium increases, which did not oscillate, and stomatal closure was abolished. Conversely, cold and abscisic acid elicited calcium oscillations in det3, and stomatal closure occurred normally. Moreover, in det3 guard cells, experimentally imposing external calcium-induced oscillations rescued stomatal closure. These data provide genetic evidence that stimulus-specific calcium oscillations are necessary for stomatal closure.
Allen
G J
GJ
Cell and Developmental Biology Section, Division of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla, CA 92093-0116, USA. gallen@biomail.ucsd.edu
Chu
S P
SP
Schumacher
K
K
Shimazaki
C T
CT
Vafeados
D
D
Kemper
A
A
Hawke
S D
SD
Tallman
G
G
Tsien
R Y
RY
Harper
J F
JF
Chory
J
J
Schroeder
J I
JI
D000040:Abscisic Acid / Q000494:pharmacology; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics / Q000502:physiology*; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling; D002462:Cell Membrane / Q000378:metabolism; D003080:Cold Temperature; D004721:Endoplasmic Reticulum / Q000378:metabolism; D017343:Genes, Plant; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008564:Membrane Potentials; D009154:Mutation; D018384:Oxidative Stress; D018515:Plant Leaves / Q000166:cytology / Q000502:physiology*; D011188:Potassium / Q000378:metabolism; D006180:Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism; D025262:Vacuolar Proton-Translocating ATPases; D014617:Vacuoles / Q000378:metabolism
D000040:Abscisic Acid; D006861:Hydrogen Peroxide; D025262:Vacuolar Proton-Translocating ATPases; D006180:Proton-Translocating ATPases; D011188:Potassium; D002118:Calcium