Cytotoxic effect and electrophysiologic activity of (R)-bgugaine, an alkylpyrrolidine alkaloid against MRC-5 fibroblasts.
The cytotoxicity and the electrophysiological effects against MRC-5 fibroblasts of 2-alkylpyrrolidine, (-)-R-bgugaine [1], a natural alkaloid, extracted from Arisarum vulgare Targ., (Araceae) were studied. This compound showed a cytotoxic potential against MRC-5 fibroblasts at 10 microg/ml. The electrophysiological study of this molecule on MRC-5 cells was carried out using the technique of patch-clamp and showed that (-)-R-bgugaine [1] has a sub-maximum effect on the outward potassium (current) at the concentration of 1 microM (0.281 microg/ml). In this study we show that R-bgugaine [1] activates the outwards potassium (current) activated by intracellular calcium.
Lamkadem
M
M
Laboratory of Phytochemistry and Pharmacognosy, Faculty of Sciences, University Mohamed Ier, Oujada, Morocco.
Melhaoui
A
A
Mimouni
J
J
Fontonge
R
R
D000470:Alkaloids / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D015221:Potassium Channels / Q000187:drug effects; D011759:Pyrrolidines / Q000494:pharmacology*
D000470:Alkaloids; D015221:Potassium Channels; D011759:Pyrrolidines; C118087:bgugaine; D002118:Calcium