Restricting expression prolongs expression of foreign genes introduced into animals by retroviruses.
If foreign genes are ubiquitously expressed in mice using a viral vector, expression is abrogated by CD8(+) cells in 2 to 4 weeks. However, if the expression of the genes is confined to skeletal muscle cells, the CD8(+) T-cell response is much weaker and expression is maintained for more than 6 weeks. These data show that restricting the expression of foreign genes to skeletal muscle cells and presumably to other cells that are inefficient at antigen presentation can prolong the expression of a foreign gene product.
Pinto
V B
VB
ABL-Basic Research Program, National Cancer Institute-Frederick Cancer Research and Development Center, Frederick, Maryland 21702-1201, USA.
Prasad
S
S
Yewdell
J
J
Bennink
J
J
Hughes
S H
SH
D000469:Alkaline Phosphatase / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D001354:Avian Leukosis Virus / Q000235:genetics*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D015870:Gene Expression; D005822:Genetic Vectors; D051379:Mice; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism* / Q000821:virology*; D010047:Ovalbumin / Q000737:chemistry / Q000276:immunology; D010452:Peptide Biosynthesis; D010455:Peptides / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000096:biosynthesis*
D010455:Peptides; D011994:Recombinant Proteins; D010047:Ovalbumin; D000469:Alkaline Phosphatase