Gene expression in rat dermal papilla cells: analysis of 2529 ESTs.
Dermal papilla (DEPA) cells are resident at the base of hair follicles and are fundamental to hair growth and development. Cultured DEPA cells, in contrast to normal fibroblast cells, are capable of inducing de novo hair follicle growth in vivo. By differential screening of a DEPA cDNA library, we have demonstrated that dermal papilla cells are different from fibroblasts at the molecular level. We further studied these cells by random sequencing of 5130 clones from the DEPA cDNA library. Fifty percent had a BLASTX E value < or =1 x 10(-25). Twenty-one percent had similarity to proteins involved in cell structure/motility with 4 of the top 10 most abundant clones encoding extracellular matrix proteins. Clones encoding growth factor molecules were also abundant. The remaining 50.7% of clones had low similarity scores, demonstrating many novel molecules. For example, we identified a new CTGF family member, the rat homologue of Elm1.
Sleeman
M A
MA
Genesis Research and Development Corporation Limited, Auckland, New Zealand. m.sleeman@genesis.co.nz
Murison
J G
JG
Strachan
L
L
Kumble
K
K
Glenn
M P
MP
McGrath
A
A
Grierson
A
A
Havukkala
I
I
Tan
P L
PL
Watson
J D
JD
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D055511:CCN Intercellular Signaling Proteins; D002478:Cells, Cultured; D018076:DNA, Complementary; D020224:Expressed Sequence Tags; D005347:Fibroblasts; D005786:Gene Expression Regulation; D015723:Gene Library; D018859:Hair Follicle / Q000166:cytology* / Q000502:physiology*; D008969:Molecular Sequence Data; D015513:Oncogene Proteins; D009928:Organ Specificity; D011518:Proto-Oncogene Proteins; D051381:Rats; D011919:Rats, Inbred Strains; D012097:Repressor Proteins / Q000235:genetics
D055511:CCN Intercellular Signaling Proteins; C492066:CCN4 protein, rat; D018076:DNA, Complementary; D015513:Oncogene Proteins; D011518:Proto-Oncogene Proteins; D012097:Repressor Proteins