Characterization of strain HY99, a novel microorganism capable of aerobic and anaerobic degradation of aniline.
We have characterized a novel microorganism, strain HY99, which is capable of aerobic and anaerobic degradation of aniline. Strain HY99 was found to aerobically metabolize aniline via catechol and 2-hydroxymuconic semialdehyde intermediates, and to transform aniline via p-aminobenzoate in anaerobic environments. Physiological and biochemical tests revealed that strain HY99 was most similar to Delftia acidovorans, but unlike D. acidovorans, strain HY99 was able to metabolize aniline under anaerobic conditions linked with nitrate reduction. Phylogenetic analysis based on 16S rDNA sequencing also revealed that strain HY99 was closely related to D. acidovorans, with 96% overall similarity.
Kahng
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Biotechnology Center for Agriculture and the Environment, Rutgers University, New Brunswick, NJ 08901-8520, USA.
Kukor
J J
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K H
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D000332:Aerobiosis; D000693:Anaerobiosis; D000814:Aniline Compounds / Q000378:metabolism*; D020562:Betaproteobacteria / Q000145:classification* / Q000254:growth & development* / Q000378:metabolism; D001673:Biodegradation, Environmental; D002244:Carbon / Q000378:metabolism; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D000814:Aniline Compounds; D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D002244:Carbon; D009584:Nitrogen; C023650:aniline