A cDNA encoding a chitinase from the epithelial cell line of chironomus tentans (Insecta, diptera) and its functional expression.
A cDNA coding for chitinase was isolated from Chironomus cells, which possesses conserved regions I and II characteristic for family 18 chitinases, a C-terminus enriched in Glu and Pro without the typical "PEST-region," putative glycosylation sites, a reduced number of C-terminal cysteines, and no typical chitin binding domain. Northern blots revealed one specific signal with an apparent size of 2.3 kb. The cDNA was expressed in the baculovirus/Spodoptera system as a His-tag fusion protein, which was secreted as a functionally active enzyme into the medium and could be separated from endogenous viral and Spodoptera-specific chitinases.
Feix
M
M
Entwicklungs- und Molekularbiologie der Tiere, Heinrich-Heine-Universität Düsseldorf, Germany.
Glöggler
S
S
Londershausen
M
M
Weidemann
W
W
Spindler
K D
KD
Spindler-Barth
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D020134:Catalytic Domain; D002460:Cell Line; D002683:Chironomidae / Q000201:enzymology*; D002688:Chitinases / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary; D004847:Epithelial Cells / Q000166:cytology; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D018411:Spodoptera / Q000166:cytology
D018076:DNA, Complementary; D011993:Recombinant Fusion Proteins; D002688:Chitinases