title: The K(+) affinity of gastric H(+),K(+)-ATPase is affected by both lipid composition and the beta-subunit.
abstract: It is generally assumed that negatively charged residues present in the alpha-subunit of gastric H(+),K(+)-ATPase are involved in K(+) binding and transport. Despite the fact that there is no difference between various species regarding these negatively charged residues, it was observed that the apparent K(+) affinity of the pig enzyme was much lower than that of the rat H(+),K(+)-ATPase. By determining the K(+)-stimulated dephosphorylation reaction of the phosphorylated intermediate K(0.5) values for K(+) of 0.12+/-0.01 and 1.73+/-0.03 mM were obtained (ratio 14.4) for the rat and the pig enzyme, respectively. To investigate the reason for the observed difference in K(+) sensitivity, both enzymes originating from the gastric mucosa were either reconstituted in a similar lipid environment or expressed in Sf9 cells. After reconstitution in K(+)-permeable phosphatidylcholine/cholesterol liposomes K(0.5) values for K(+) of 0.16+/-0.01 and 0.35+/-0.05 mM for the rat and pig enzyme respectively were measured (ratio 2.2). After expression in Sf9 cells the pig gastric H(+),K(+)-ATPase still showed a 4.1 times lower K(+) sensitivity than that of the rat enzyme. This means that the difference in K(+) sensitivity of the rat and pig gastric H(+), K(+)-ATPase is not only due to a different lipid composition but also to the structure of either the alpha- or beta-subunit. Expression of hybrid enzymes in Sf9 cells showed that the difference in K(+) sensitivity between the rat and pig gastric H(+),K(+)-ATPase is primarily due to differences in the beta-subunit.
authors.affiliation: Department of Biochemistry, Institute of Cellular Signalling, University of Nijmegen, The Netherlands.
authors.forename: H P
authors.initials: HP
authors.lastname: Hermsen
authors.forename: H G
authors.initials: HG
authors.lastname: Swarts
authors.forename: L
authors.initials: L
authors.lastname: Wassink
authors.forename: F J
authors.initials: FJ
authors.lastname: Dijk
authors.forename: M T
authors.initials: MT
authors.lastname: Raijmakers
authors.forename: C H
authors.initials: CH
authors.lastname: Klaassen
authors.forename: J B
authors.initials: JB
authors.lastname: Koenderink
authors.forename: M
authors.initials: M
authors.lastname: Maeda
authors.forename: J J
authors.initials: JJ
authors.lastname: De Pont
mesh_terms: D000818:Animals; D002460:Cell Line; D005753:Gastric Mucosa / Q000201:enzymology*; D017506:H(+)-K(+)-Exchanging ATPase / Q000737:chemistry / Q000378:metabolism*; D050356:Lipid Metabolism; D010766:Phosphorylation; D011188:Potassium / Q000378:metabolism*; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013552:Swine
chemical_list: D011994:Recombinant Proteins; D017506:H(+)-K(+)-Exchanging ATPase; D011188:Potassium