title: Enzymatic properties of sialidase from the ovary of the starfish, Asterina pectinifera.
abstract: A sialidase [EC 3.2.1 18] was isolated and highly purified from the ovary of the starfish, Asterina pectinifera, and its enzymatic properties were compared with those of human placental sialidase. The final preparation gave one broad protein band corresponding to sialidase activity on polyacrylamide gel electrophoresis. The molecular weight of the enzyme was 360000 by HPLC on Sigma Chrome GFC-1300 and Sephadex G-150 column chromatography, and 55000 by SDS-PAGE, suggesting the presence of a hexamer in the native protein. The optimum pH was between 3.0 and 4.0, and the enzyme liberated sialyl residues from the following compounds: alpha(2-3) and alpha(2-6) sialyllactose, colominic acid, fetuin, transferrin, gangliosides GM3, GD1a and GD1b. The enzyme was strongly inhibited by 4-aminophenyl and methyl thio-glycosides of sialic acid, but not by those glycosides of 5-amino sialic acid or sialic acid methyl ester. The enzyme was also highly inhibited by sulfated glucan and glycosaminoglycans. The substrate specificity and the effects of inhibitors on starfish sialidase were very similar to those of human placental sialidase.
authors.affiliation: Department of Health Chemistry, Niigata College of Pharmacy, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Iriyama
authors.forename: N
authors.initials: N
authors.lastname: Takeuchi
authors.forename: T
authors.initials: T
authors.lastname: Shiraishi
authors.forename: K
authors.initials: K
authors.lastname: Izumi
authors.forename: M T
authors.initials: MT
authors.lastname: Sawada
authors.forename: N
authors.initials: N
authors.lastname: Takahashi
authors.forename: K
authors.initials: K
authors.lastname: Furuhata
authors.forename: H
authors.initials: H
authors.lastname: Ogura
authors.forename: Y
authors.initials: Y
authors.lastname: Uda
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D015394:Molecular Structure; D009439:Neuraminidase / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D010053:Ovary / Q000201:enzymology; D010920:Placenta / Q000201:enzymology; D013215:Starfish / Q000201:enzymology*; D013379:Substrate Specificity; D013431:Sulfates / Q000494:pharmacology
chemical_list: D013431:Sulfates; D009439:Neuraminidase