title: New virulence-activated and virulence-repressed genes identified by systematic gene inactivation and generation of transcriptional fusions in Bordetella pertussis.
abstract: An in silico scan of the partially completed genome sequence of Bordetella pertussis and analyses of transcriptional fusions generated with a new integrational vector were used to identify new potential virulence genes. The genes encoding a putative siderophore receptor, adhesins, and an autotransporter protein appeared to be regulated in a manner similar to Bordetella virulence genes by the global virulence regulator BvgAS. In contrast, the gene encoding a putative intimin-like protein appeared to be repressed under conditions of virulence.
authors.affiliation: INSERM U447, Institut de Biologie de Lille, Institut Pasteur de Lille, 59019 Lille Cedex, France.
authors.forename: R
authors.initials: R
authors.lastname: Antoine
authors.forename: S
authors.initials: S
authors.lastname: Alonso
authors.forename: D
authors.initials: D
authors.lastname: Raze
authors.forename: L
authors.initials: L
authors.lastname: Coutte
authors.forename: S
authors.initials: S
authors.lastname: Lesjean
authors.forename: E
authors.initials: E
authors.lastname: Willery
authors.forename: C
authors.initials: C
authors.lastname: Locht
authors.forename: F
authors.initials: F
authors.lastname: Jacob-Dubuisson
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D001886:Bordetella pertussis / Q000235:genetics* / Q000472:pathogenicity*; D004352:Drug Resistance, Microbial / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D020868:Gene Silencing; D005798:Genes, Bacterial; D005839:Gentamicins; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D013489:Suppression, Genetic; D014158:Transcription, Genetic; D014774:Virulence / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D005839:Gentamicins; D011993:Recombinant Fusion Proteins