Identification of a new HLA-DRB4 allele (DRB4*01033) by PCR-SSP and direct sequencing.
In this report, we describe the identification of a novel DRB4*01 allele, DRB4*01033, found in two Spanish Caucasian individuals. The new allele was detected during routine HLA typing by an unusual pattern of amplification obtained by polymerase chain reaction using sequence-specific primers (PCR-SSP) that did not match with any of the previously described DRB4 alleles. In order to establish the polymorphism responsible for this pattern exons 2 and 3 of the DRB4 locus were amplified and directly sequenced. The new DRB4*01 allele is identical to DRB4*0103101 except for a single nucleotide substitution in codon 78 (TAC-->TAT). This nucleotide change does not cause an amino acid change as both triplets code for a tyrosine.
Palou
E
E
Servei d'Immunohematologia, Centre de Transfusió i Banc de Teixits, Barcelona, Spain. epalou@hg.vhebron.es
Nogués
N
N
Gil
J
J
Ribera
A
A
D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers; D006681:HLA-D Antigens / Q000235:genetics*; D006684:HLA-DR Antigens / Q000235:genetics*; D015804:HLA-DR4 Antigen; D059845:HLA-DRB4 Chains; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D016415:Sequence Alignment
D017931:DNA Primers; D006681:HLA-D Antigens; D006684:HLA-DR Antigens; D015804:HLA-DR4 Antigen; D059845:HLA-DRB4 Chains; C420209:HLA-DRB4*01033 antigen