Genomic organization of a new candidate tumor suppressor gene, LRP1B.
LRP1B is a novel candidate tumor suppressor gene that is inactivated by genetic and transcript alterations in nearly 50% of non-small-cell lung cancer cell lines. The gene-encoded protein is highly homologous to the gigantic lipoprotein receptor-related protein 1 (LRP1) that belongs to the family of low-density lipoprotein receptors. Using a combination of PCR-based genome walking and long-distance interexon PCR, we have determined the genomic organization of LRP1B and built a contiguous array of BAC clones spanning this gene. A total of 91 exons, varying in size from 77 bases (exon 87) to 1899 bases (exon 91), were identified in the more than 500-kb-long gene sequence. Comparative analysis of the genomic structures of LRP1B and the homologous LRP1 gene revealed a striking similarity in the location and sizes of their exons.
Liu
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Laboratory of Cancer Genetics, Department of Genetics, University of Pennsylvania School of Medicine, 422 Curie Boulevard, Philadelphia, Pennsylvania 19104, USA.
Musco
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Lisitsina
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Yaklichkin
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Lisitsyn
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D022202:Chromosomes, Artificial, Bacterial; D005091:Exons; D016147:Genes, Tumor Suppressor; D006801:Humans; D007438:Introns; D026503:Low Density Lipoprotein Receptor-Related Protein-1; D008969:Molecular Sequence Data; D011971:Receptors, Immunologic / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D026503:Low Density Lipoprotein Receptor-Related Protein-1; D011971:Receptors, Immunologic