title: Determination of the enantiomers of 3-tert.-butylamino-1,2-propanediol by high-performance liquid chromatography coupled to evaporative light scattering detection.
abstract: A method for the separation and quantitation of the enantiomers of 3-tert.-butylamino-1,2-propanediol by high-performance liquid chromatography and evaporative light scattering detection has been developed. Separation of the enantiomers was performed in normal-phase liquid chromatography on a Chiralpak AS chiral stationary phase. The influence of the gas nature, gas pressure and temperature of the drift tube of the evaporative light scattering detector on the detection sensitivity was investigated. The method was validated in terms of linearity, limit of quantitation, accuracy and precision. The enantiomeric excess of (S)-3-tert.-butylamino-1,2-propanediol, used for the industrial synthesis of (S)-timolol, was measured from 0 to 94%.
authors.affiliation: Department of Analytical Pharmaceutical Chemistry, Institute of Pharmacy, University of Liège, Belgium. b.toussaint@ulg.ac.be
authors.forename: B
authors.initials: B
authors.lastname: Toussaint
authors.forename: A L
authors.initials: AL
authors.lastname: Duchateau
authors.forename: S
authors.initials: S
authors.lastname: van der Wal
authors.forename: A
authors.initials: A
authors.lastname: Albert
authors.forename: P
authors.initials: P
authors.lastname: Hubert
authors.forename: J
authors.initials: J
authors.lastname: Crommen
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008027:Light; D011409:Propylene Glycols / Q000032:analysis*; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D013237:Stereoisomerism
chemical_list: C418769:3-tert-butylamino-1,2-propanediol; D011409:Propylene Glycols