Characterization of a nuclear factor that binds to AP1-like element in the rat p53 promoter during liver regeneration.
The transcription level of the rat p53 gene increases at 5-12 h in the regenerating liver after partial hepatectomy. It was previously reported that an activator protein 1 (AP1)-like element (-264--284) mediated the induced transcription of the rat p53 gene during liver regeneration. In this study, we characterize the protein binding to the AP1-like element by various methods. Oligonucleotide competition assays showed that the binding protein did not require AP1 consensus sequence. Therefore, the binding protein is not an AP1 family protein. Zn(2+) was required for maximum DNA-binding activity of the protein, suggesting that the binding protein contains zinc fingers. The binding protein was highly resistant to denaturant. Even 1.8 M urea did not eliminate the protein-DNA complexes. In addition, the binding protein was stable up to 55 degrees C. The protein-DNA complexes were abolished in the presence of 0.6 M NaCl and higher. Protease clipping assay showed that the protein had a protease-resistant core DNA binding domain. These results provided new insights into the structure of the protein that binds to the AP1-like element of the p53 promoter during liver regeneration.
Lee
M
M
Department of Chemistry, Seoul National University, Seoul, Korea.
Yu
S
S
Park
J S
JS
D000818:Animals; D010450:Endopeptidases / Q000378:metabolism; D005260:Female; D006498:Hepatectomy; D008115:Liver Regeneration; D009687:Nuclear Proteins / Q000378:metabolism*; D009994:Osmolar Concentration; D011485:Protein Binding; D051381:Rats; D017208:Rats, Wistar; D013696:Temperature; D018808:Transcription Factor AP-1 / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*; D014508:Urea; D015032:Zinc / Q000378:metabolism
D009687:Nuclear Proteins; D018808:Transcription Factor AP-1; D016159:Tumor Suppressor Protein p53; D014508:Urea; D010450:Endopeptidases; D015032:Zinc