Hemolipodialysis attenuates oxidative stress and removes hydrophobic toxins.
Uremic patients undergoing hemodialysis often have increased oxidant stress and accumulation of uremic toxins. Hemodialysis, per se, often can exacerbate oxidant stress and may be inefficient at removing hydrophobic or protein bound toxins. We describe a new hemodialytic method that incorporates liposomes and antioxidants to remove hydrophobic/uremic toxins and minimize free radical mediated damage. In vitro experiments measured advanced oxidation protein products (AOPP), malonaldehyde, reactive carbonyls, and the removal of platelet activating factor (PAF) and bilirubin during extracorporeal circulation with or without liposomes. We observed a significant reduction of oxidation products as well as a significant removal of PAF and bilirubin compared to normal hemodialysis.
Wratten
M L
ML
Clinical and Laboratory Research Department, Bellco SpA, Mirandola, Italy.
Sereni
L
L
Tetta
C
C
D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use; D001205:Ascorbic Acid / Q000627:therapeutic use; D001663:Bilirubin / Q000097:blood; D001798:Blood Proteins / Q000737:chemistry; D002417:Cattle; D005112:Extracorporeal Circulation; D016166:Free Radical Scavengers / Q000627:therapeutic use; D006801:Humans; D008081:Liposomes / Q000627:therapeutic use*; D008315:Malondialdehyde / Q000097:blood; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology*; D010713:Phosphatidylcholines / Q000627:therapeutic use; D010743:Phospholipids / Q000627:therapeutic use; D010972:Platelet Activating Factor / Q000737:chemistry; D006435:Renal Dialysis / Q000295:instrumentation / Q000379:methods*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D014118:Toxins, Biological / Q000097:blood*; D014511:Uremia / Q000097:blood / Q000628:therapy; D014810:Vitamin E / Q000627:therapeutic use
D000975:Antioxidants; D001798:Blood Proteins; D016166:Free Radical Scavengers; D008081:Liposomes; D010713:Phosphatidylcholines; D010743:Phospholipids; D010972:Platelet Activating Factor; D014118:Toxins, Biological; D014810:Vitamin E; D012710:Serum Albumin, Bovine; D008315:Malondialdehyde; D001205:Ascorbic Acid; D001663:Bilirubin