title: Pseudomonas aeruginosa mediated apoptosis requires the ADP-ribosylating activity of exoS.
abstract: Pseudomonas aeruginosa is an opportunistic bacterial pathogen that primarily infects immunocompromised individuals and patients with cystic fibrosis. Using a tissue culture system, invasive strains of P. aeruginosa were discovered to induce apoptosis at high frequency in HeLa and other epithelial and fibroblast cell lines. This apoptotic phenotype in the infected cells was determined by several criteria including (i) visual changes in cell morphology, (ii) induction of chromatin condensation and nuclear marginalization, (iii) the presence of a high percentage of cells with subG1 DNA content, and (iv) activation of caspase-3 activity. Induction of the type III secretion machinery, but not invasion of P. aeruginosa is required for induction of apoptosis. The apoptosis phenotype is independent of the cytoskeletal rearrangements that occur in the host cell early after infection. Mutants in P. aeruginosa exoS fail to induce apoptosis and complementation with wild-type exoS restored the apoptosis-inducing capacity, demonstrating that ExoS is the effector molecule. Analysis of exoS activity mutants shows that the ADP-ribosylating capacity of ExoS is essential for inducing the apoptotic pathway.
authors.affiliation: Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA1.
authors.forename: Melissa R
authors.initials: MR
authors.lastname: Kaufman
authors.affiliation: Department of Molecular Genetics and Microbiology, PO Box 100266, University of Florida, Gainesville, FL 32610, USA2.|Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA1.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Jia
authors.affiliation: Department of Molecular Genetics and Microbiology, PO Box 100266, University of Florida, Gainesville, FL 32610, USA2.|Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA1.
authors.forename: Lin
authors.initials: L
authors.lastname: Zeng
authors.affiliation: Department of Molecular Genetics and Microbiology, PO Box 100266, University of Florida, Gainesville, FL 32610, USA2.|Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA1.
authors.forename: Unhwan
authors.initials: U
authors.lastname: Ha
authors.affiliation: Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA1.
authors.forename: Marie
authors.initials: M
authors.lastname: Chow
authors.affiliation: Department of Molecular Genetics and Microbiology, PO Box 100266, University of Florida, Gainesville, FL 32610, USA2.|Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA1.
authors.forename: Shouguang
authors.initials: S
authors.lastname: Jin
mesh_terms: D036002:ADP Ribose Transferases / Q000378:metabolism*; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D017209:Apoptosis; D001427:Bacterial Toxins; D002460:Cell Line; D048429:Cell Size; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D011550:Pseudomonas aeruginosa / Q000472:pathogenicity* / Q000502:physiology*
chemical_list: D001427:Bacterial Toxins; D000246:Adenosine Diphosphate Ribose; D036002:ADP Ribose Transferases; C444820:exoenzyme S