Cholecystokinin receptor imaging using an octapeptide DTPA-CCK analogue in patients with medullary thyroid carcinoma.
Cholecystokinin (CCK)-B receptors have been demonstrated on a high percentage of medullary thyroid carcinomas (MTC) in vitro. After encouraging results both in vitro and in animal studies, we studied the efficacy of an octapeptide [111In-DTPA]-CCK analogue in seven patients with MTC. In four of five patients in whom serum calcitonin levels were monitored, a significant rise was found following the injection, indicating retained biological activity of the radiopeptide. In all patients there was visualization of the CCK-B receptor-positive stomach. In one of two patients with known MTC lesions, some of the lesions were visualized; in addition some lesions were visualized in one of the five other patients who had elevated serum tumour markers but negative localizing studies. Radioactivity in the presumed tumour sites was still present at 48 h p.i. The uptake in the presumed tumour sites and stomach was low. Background radioactivity dropped rapidly owing to urinary excretion. After 1 h, breakdown products of the labelled analogue predominated both in urine and in serum, and virtually no intact peptide was present.


CONCLUSIONS
(1) the CCK-B receptor-positive gastric mucosa and presumed MTC lesions could be visualized in patients using an octapeptide [111In-DTPA]-CCK analogue that is probably internalized, proving the feasibility of CCK-B receptor imaging in vivo; (2) there was a relatively low uptake of the CCK analogue in the strongly CCK receptor positive stomach, and rapid degradation of the peptide in serum.
Kwekkeboom
D J
DJ
Department of Nuclear Medicine, University Hospital Rotterdam, The Netherlands.
Bakker
W H
WH
Kooij
P P
PP
Erion
J
J
Srinivasan
A
A
de Jong
M
M
Reubi
J C
JC
Krenning
E P
EP
D018276:Carcinoma, Medullary / Q000000981:diagnostic imaging*; D006801:Humans; D007205:Indium Radioisotopes; D004369:Pentetic Acid; D011877:Radionuclide Imaging; D043887:Receptor, Cholecystokinin B; D011949:Receptors, Cholecystokinin / Q000032:analysis*; D012844:Sincalide / Q000378:metabolism*; D013964:Thyroid Neoplasms / Q000000981:diagnostic imaging*; D014018:Tissue Distribution
D007205:Indium Radioisotopes; D043887:Receptor, Cholecystokinin B; D011949:Receptors, Cholecystokinin; D004369:Pentetic Acid; D012844:Sincalide