title: Phylogenetic position of parabasalid symbionts from the termite Calotermes flavicollis based on small subunit rRNA sequences.
abstract: Small subunit rDNA genes were amplified by polymerase chain reaction using specific primers from mixed-population DNA obtained from the whole hindgut of the termite Calotermes flavicollis. Comparative sequence analysis of the clones revealed two kinds of sequences that were both from parabasalid symbionts. In a molecular tree inferred by distance, parsimony and likelihood methods, and including 27 parabasalid sequences retrieved from the data bases, the sequences of the group II (clones Cf5 and Cf6) were closely related to the Devescovinidae/Calonymphidae species and thus were assigned to the Devescovinidae Foaina. The sequence of the group I (clone Cf1) emerged within the Trichomonadinae and strongly clustered with Tetratrichomonas gallinarum. On the basis of morphological data, the Monocercomonadidae Hexamastix termitis might be the most likely origin of this sequence.
authors.affiliation: Laboratoire de Biologie Comparée des Protistes, UPRESA CNRS 6023, Aubière, France.
authors.forename: D
authors.initials: D
authors.lastname: Gerbod
authors.forename: V P
authors.initials: VP
authors.lastname: Edgcomb
authors.forename: C
authors.initials: C
authors.lastname: Noël
authors.forename: P
authors.initials: P
authors.lastname: Delgado-Viscogliosi
authors.forename: E
authors.initials: E
authors.lastname: Viscogliosi
mesh_terms: D000818:Animals; D016054:DNA, Protozoan / Q000235:genetics / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000235:genetics / Q000302:isolation & purification; D056890:Eukaryota / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D007422:Intestines / Q000469:parasitology; D020049:Isoptera / Q000469:parasitology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D016053:RNA, Protozoan / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D013559:Symbiosis; D016834:Trichomonadida / Q000145:classification / Q000235:genetics / Q000302:isolation & purification
chemical_list: D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D016053:RNA, Protozoan; D012335:RNA, Ribosomal