The ZF87/MAZ transcription factor functions as a growth suppressor in fibroblasts.
ZF87/MAZ is a zinc finger transcription factor that activates expression of tissue-specific genes and represses expression of the c-myc proto-oncogene. Infection of NIH3T3 fibroblasts with a retrovirus expressing ZF87/MAZ leads to a significant reduction in G418-resistant colonies, compared to cells infected with a retroviral control. Further, only a small fraction of the G418-resistant colonies express ZF87/MAZ. When the ZF87/MAZ-expressing colonies are expanded, they demonstrate a slow growth phenotype, a delayed transit through G1 phase and a decrease in endogenous c-myc gene expression and cyclin A and cyclin E protein levels. Consistent with a partial G1 arrest, the ZF87/MAZ-expressing cells show a reduced sensitivity to the S phase specific chemotherapeutic agent camptothecin. These data indicate that ZF87/MAZ is a growth suppressor protein in nontransformed cells, in part, by affecting the levels of key cell cycle regulatory proteins.
Stubbs
M C
MC
Department of Orthopaedic Surgery, Thomas Jefferson University, Philadelphia, PA 19107, USA.
Min
I
I
Izzo
M W
MW
Rallapalli
R
R
Derfoul
A
A
Hall
D J
DJ
D016475:3T3 Cells; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D002166:Camptothecin / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media; D019925:Cyclin A / Q000235:genetics / Q000378:metabolism; D019927:Cyclin E / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins; D016193:G1 Phase; D015870:Gene Expression; D017930:Genes, Reporter; D016259:Genes, myc / Q000235:genetics; D005839:Gentamicins / Q000494:pharmacology; D015151:Immunoblotting; D051379:Mice; D012190:Retroviridae / Q000235:genetics; D016196:S Phase; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D016335:Zinc Fingers
D000900:Anti-Bacterial Agents; D000972:Antineoplastic Agents, Phytogenic; D003470:Culture Media; D019925:Cyclin A; D019927:Cyclin E; D004268:DNA-Binding Proteins; D005839:Gentamicins; D014157:Transcription Factors; C076035:c-MYC-associated zinc finger protein; C010680:antibiotic G 418; D002166:Camptothecin