[Modeling heterochromatin regions in transgenic mice].
Transgenic mice carrying bovine satellite DNA IV were obtained. The size of the transgene integrated into the mouse genome was approximately 390 kb (about 100 transgene copies) as determined by a semiquantitative PCR. Restriction analysis with isoschizomeric restrictases HpaII and MspI, showed that the alien DNA was methylated. In the genome of a transgenic founder male, two integration sites for satellite DNA IV were revealed by in situ hybridization and in situ PCR. These sites are situated on two different chromosomes: in pericentromeric heterochromatin and within a chromosomal arm. In transgenic mice, de novo formation of heterochromatin regions (C-block and the CMA3 disk within the centromeric heterochromatin of another chromosome) was revealed by C-banding and staining with chromomycin A3. This formation is not characteristic of mice, because their chromosomes normally contain no interstitial C-blocks or sequences intensely stained by chromomycin A3.
Popov
A V
AV
Institute of Genetics and Breeding of Farm Animals, Russian Academy of Agricultural Sciences, St. Petersburg, Russia.
Smirnov
A F
AF
Suchkova
I O
IO
Baranova
T V
TV
Sorokin
A V
AV
Gaĭtskhoki
V S
VS
Patkin
E L
EL
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D017931:DNA Primers; D004276:DNA, Satellite / Q000235:genetics; D018703:Founder Effect; D006570:Heterochromatin / Q000235:genetics*; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic; D008957:Models, Genetic; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D004276:DNA, Satellite; D006570:Heterochromatin