The study of cell-mediated immune response in recurrent vulvovaginal candidiasis.
The aim of this work was to examine in vitro the ability of cells from patients with recurrent vulvovaginal candidiasis (RVVC) to cell-mediated immune response. Peripheral blood mononuclear cells (PBMC) and whole blood cells (WBC) of 37 RVVC patients in acute infection and 14 in remission were examined for the ability to proliferation and cytokines production (IFN, TNF, IL-6). As a control, a group of 25 healthy women were examined. The cells were stimulated with Candida antigen (HKCA), LPS and PHA. To indicate the level of cytokines, the following cell-lines were used: A549 for IFN, WEHI 164 for TNF and 7TD1 for IL-6. The proliferation/death of cells was determined by colorimetric test using MTT. Distinct suppression of cell-mediated immune response (CMI) was shown in all patients comparing to the control. Greatest suppression was found in the acute phase of the disease. The ability of cells to proliferate and produce IFN increases only in remission. The data seem to suggest that in this phase of disease, the ability of cell-mediated immune response is restored. It was also indicated that IFN may take part in protection against Candida infection.
Nawrot
U
U
Department of Microbiology Medical University of Wrocław, Poland.
Grzybek-Hryncewicz
K
K
Zielska
U
U
Czarny
A
A
Podwińska
J
J
D000328:Adult; D000946:Antigens, Fungal / Q000276:immunology; D001681:Biological Assay; D002176:Candida albicans / Q000276:immunology; D002181:Candidiasis, Vulvovaginal / Q000276:immunology*; D018929:Cell Culture Techniques; D002460:Cell Line; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006801:Humans; D007111:Immunity, Cellular; D007372:Interferons / Q000096:biosynthesis*; D015850:Interleukin-6 / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008213:Lymphocyte Activation; D012008:Recurrence; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D000946:Antigens, Fungal; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D007372:Interferons