title: Effect of amino acid mixtures on nasal allergic responses induced by toluene diisocyanate in mice.
abstract: We studied the effect of various amino acid mixtures on nasal allergy induced by the intranasal application of toluene diisocyanate (TDI) in mice. In Experiment 1 (Exp. 1), mice were fed a 25% casein, soy protein isolate (SPI), egg white protein (EW) or gluten diet. In Experiment 2 (Exp. 2), mice were fed a 25% amino acid mixture diets patterned after casein (AA-casein), SPI (AA-SPI), EW protein (AA-EW) or gluten (AA-gluten). In Experiment 3 3 (Exp. 3) we modified the glutamine/glutamic acid (Gln/Glu) concentrations in the amino acid mixtures. Mice were fed a 25% AA-SPI, low Gln/Glu AA-SPI (LG-AA-SPI), AA-EW or high Gln/Glu AA-EW (HG-AA-EW) diet. At the 5th week, mice were divided into sensitized (sen-) and non-sensitized (ns-) groups. The mice in sensitized groups were treated with two courses of intranasal application of toluene diisocyanate (TDI) in ethyl acetate for 5 consecutive days, separated by 9 days rest. The non-sensitized groups of mice were treated with a vehicle. Nine days after the second sensitization, all mice were provoked by TDI. Nasal responses and serum IgE concentration were studied. The findings of Exp. 1 showed that the sen-EW group exhibited a lower body weight gain, higher nasal symptom score and higher IgE concentration than the other sensitized groups. The findings dings of Exp. 2 showed that the sen-EW group had a lower body weight gain, higher nasal symptom score and higher IgE concentration than the other sensitized groups. In Exp. 3, the AA-EW group showed a higher total nasal score and IgE concentration than the HG-AA-EW group, however, the findings of LG-AA-SPI and AA-SPI were similar. These findings demonstrated that amino acid mixtures affect nasal allergy induced by the intranasal application of TDI in mice.
authors.affiliation: Department of Nutrition, University of Tokushima School of Medicine, Japan.
authors.forename: T H
authors.initials: TH
authors.lastname: Chen
authors.forename: K
authors.initials: K
authors.lastname: Takeshita
authors.forename: K
authors.initials: K
authors.lastname: Sakai
authors.forename: T
authors.initials: T
authors.lastname: Komatsu
authors.forename: Y
authors.initials: Y
authors.lastname: Kishino
authors.forename: S
authors.initials: S
authors.lastname: Yamamoto
mesh_terms: D000281:Administration, Intranasal; D000596:Amino Acids / Q000008:administration & dosage*; D000818:Animals; D004044:Dietary Proteins / Q000008:administration & dosage*; D005260:Female; D006967:Hypersensitivity / Q000188:drug therapy* / Q000276:immunology; D051379:Mice; D014051:Toluene 2,4-Diisocyanate / Q000008:administration & dosage* / Q000276:immunology
chemical_list: D000596:Amino Acids; D004044:Dietary Proteins; D014051:Toluene 2,4-Diisocyanate