Serotypes and subgroups of rotavirus isolated from children in central Brazil.
Group A rotavirus, obtained from children of Goiânia, Brazil, during 1987-1994, were analyzed for subgroup and G serotype by enzyme-linked immunosorbent assay with monoclonal antibodies. The index of serotyping obtained was 61.4% with the following proportions: G1--19.7%, G2--28.0%, G3--9.8%, G4--1.5%, and G5--2.3%. It was observed that G1 occurred from 1987 to 1989 and from 1993 to 1994, and G2 from 1990 to 1993. About 94% of the samples (85/90) could be subgrouped with the following results: 55.5% for SG II, 7.8% SG I, and 31.1% for SG non-I-non-II. Unusual relationship patterns were also detected among serotypes, subgroups, and profiles of electropherotypes in 57.0% of the samples: 20 of them were G2/SG II/"long" profile. The results suggest that variation in temporal and regional characteristics should be considered in the development of rotavirus vaccine.
Cardoso das
D
D
Lab. Virologia/IPTSP/UFG-Goiânia-GO, Brazil. divina@netgo.com.br
Soares
C M
CM
Azevedo
M S
MS
Leite
J P
JP
Munford
V
V
Rácz
M L
ML
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000097:blood; D001938:Brazil / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D003967:Diarrhea / Q000453:epidemiology / Q000517:prevention & control / Q000821:virology*; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000821:virology*; D005260:Female; D006801:Humans; D017589:Immunization Programs / Q000458:organization & administration; D007223:Infant; D008297:Male; D012401:Rotavirus / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology / Q000517:prevention & control / Q000821:virology*; D012703:Serotyping
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral