Nonylphenol provokes a vesiculation of the Golgi apparatus in three fish epidermis cultures.
The aim of this work was to study the effects of nonylphenol and waste water on the cell ultrastructure of fish skin. Therefore, besides a recently established primary cell culture and a cell line, an epidermal tissue culture of fish was developed and tested. In all three systems a prominent vesiculation of the Golgi apparatus was observed after exposure to nonylphenol, which has not been described before and therefore strongly suggests an effect that might indicate exposure to nonylphenol and/or related substances. The Epithelial papulosum cyprini cell line was the most sensitive to nonylphenol, followed by the primary cell culture of epidermis cells and then the explant tissue culture. The vesiculation of the Golgi apparatus was accompanied by degenerative changes in the two cell cultures only. The lack of degenerative changes in the cells of the tissue culture was discussed with respect to the presence of differentiated cells that might better be able to protect themselves by mucous or by an activated xenobiotic metabolism. In a second type experiment, a waste water sample containing small concentrations of nonylphenol was applied to the cultures. It did not lead to a vesiculation of the Golgi apparatus, probably because the nonylphenol concentrations in the waste water were too low to induce the vesiculation. The cultures exposed to waste water revealed unspecific degenerative cellular changes. Additionally, explant cultures were prepared from fish that had survived a 6-month exposure to polluted river water. In these cultures a higher number of mitochondria containing myelin bodies were observed when compared to control cultures. Consequently, exposure to polluted water containing a mixture of substances in vitro and in vivo was found to lead to degenerative alterations in the ultrastructure of the cells.
Lamche
G
G
Centre for Fish and Wildlife Health, Institute of Animal Pathology, Laenggassstrasse 122, Bern, CH-3012, Switzerland.
Burkhardt-Holm
P
P
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D000078404:Epidermal Cells; D004817:Epidermis / Q000187:drug effects* / Q000648:ultrastructure; D006056:Golgi Apparatus / Q000187:drug effects* / Q000648:ultrastructure; D008854:Microscopy, Electron; D017686:Oncorhynchus mykiss / Q000502:physiology*; D010636:Phenols / Q000494:pharmacology*; D012722:Sewage / Q000009:adverse effects* / Q000737:chemistry; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000633:toxicity
D010636:Phenols; D012722:Sewage; D014874:Water Pollutants, Chemical; C025256:nonylphenol