title: Adjuvant effects of various lipopeptides and interferon-gamma on the humoral immune response of chickens.
abstract: The adjuvant effects of various lipopeptides and recombinant chicken interferon gamma (IFN-gamma) on the humoral immune response of laying hens was investigated in four immunization studies. We used the lipopeptide Pam3Cys-Ser-(Lys)4 (PCSL), the conjugate P-Th1 consisting of the lipopeptide P3CS and the T-helper epitope Th1 (FISEAIIHVLHSRHPG), and the conjugate P-Th2 of the lipopeptide P3CSS and the T-helper epitope Th2, which corresponds to the peptide EWEFVNTPPLV, as adjuvants. Human serum albumin (HSA), recombinant bovine somatotropin (RBST), and human immunoglobulin G (IgG) served as antigens in the different experiments. All tested adjuvants enhanced the humoral immune response with various intensities. Chickens showed high antibody titers after the immunization with HSA even without adjuvant, but the adjuvant effects of PCSL and the combination of PCSL and recombinant chicken interferon-gamma (IFN-gamma) were much more pronounced using the antigens RBST and IgG. Especially after the third immunization, higher titers of antibodies were induced by the coadministration of P-Th1 and, to a greater extent, by the combination of PCSL and P-Th1 compared with the use of PCSL. Also, chickens that had received PCSL and P-Th2 showed the highest immune response, even after the second booster. The average concentrations of chicken immunoglobulin Y were significantly higher in 5-mo-old chickens (9.4 mg/mL serum and 10.1 mg/mL egg yolk) compared with 9-mo-old chickens (5.9 mg/mL serum and 5.1 mg/mL egg yolk). The specific serum antibody response was higher in the older chickens than in the younger chickens. Because chicken antibodies are likely to be used increasingly for diagnostic and therapy in the future, lipopeptides and recombinant chicken IFN-gamma may find many applications as adjuvants, thus contributing to the welfare of experimental animals.
authors.affiliation: Institut für Physiologie, Physiologische Chemie und Tierernährung, Tierärztliche Fakultät, Universität München, Germany. erhard@rz.uni-leipzig.de
authors.forename: M H
authors.initials: MH
authors.lastname: Erhard
authors.forename: P
authors.initials: P
authors.lastname: Schmidt
authors.forename: P
authors.initials: P
authors.lastname: Zinsmeister
authors.forename: A
authors.initials: A
authors.lastname: Hofmann
authors.forename: U
authors.initials: U
authors.lastname: Münster
authors.forename: B
authors.initials: B
authors.lastname: Kaspers
authors.forename: K H
authors.initials: KH
authors.lastname: Wiesmüller
authors.forename: W G
authors.initials: WG
authors.lastname: Bessler
authors.forename: M
authors.initials: M
authors.lastname: Stangassinger
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000906:Antibodies / Q000097:blood; D000917:Antibody Formation / Q000187:drug effects*; D000941:Antigens / Q000276:immunology; D002417:Cattle; D002645:Chickens / Q000276:immunology*; D004151:Dipeptides / Q000008:administration & dosage / Q000494:pharmacology; D004530:Egg Yolk / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D019382:Human Growth Hormone / Q000276:immunology; D006801:Humans; D007114:Immunization; D007074:Immunoglobulin G / Q000276:immunology; D007136:Immunoglobulins / Q000032:analysis / Q000097:blood; D007371:Interferon-gamma / Q000494:pharmacology*; D008074:Lipoproteins / Q000008:administration & dosage / Q000494:pharmacology*; D011994:Recombinant Proteins; D012709:Serum Albumin / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000906:Antibodies; D000941:Antigens; D004151:Dipeptides; C016512:IgY; D007074:Immunoglobulin G; D007136:Immunoglobulins; D008074:Lipoproteins; D011994:Recombinant Proteins; D012709:Serum Albumin; C061108:N-palmitoyl-S-(2,3-bis(palmitoyloxy)propyl)cysteinyl-seryl-lysyl-lysyl-lysyl-lysine; D019382:Human Growth Hormone; D007371:Interferon-gamma; C047210:2,3-bis-(palmitoyloxy)-2-propyl-N-palmitoyl-cysteinylserine