title: The mitochondrial genome of the primary screwworm fly Cochliomyia hominivorax (Diptera: Calliphoridae).
abstract: The complete sequence of the mitochondrial genome of the screwworm Cochliomyia hominivorax was determined. This genome is 16,022 bp in size and corresponds to a typical Brachycera mtDNA. A Serine start codon for COI and incomplete termination codons for COII, NADH 5 and NADH 4 genes were described. The nucleotide composition of C. hominivorax mtDNA is 77% AT-rich, reflected in the predominance of AT-rich codons in protein-coding genes. Non-optimal codon usage was commonly observed in C. hominivorax mitochondrial genes. Phylogenetic analysis distributed the Acalypterate species as a monophyletic group and assembled the C. hominivorax (Calyptratae) and the Acalyptratae in a typical Brachycera cluster. The identification of diagnostic restriction sites on the sequenced mitochondrial genome and the correlation with previous RFLP analysis are discussed.
authors.affiliation: Laboratório de Genética Animal,Laboratório Genoma, Instituto de Biologia, Universidade Estadual de Campinas - UNICAMP, Campinas, São Paulo, Brazil.
authors.forename: A C
authors.initials: AC
authors.lastname: Lessinger
authors.forename: A C
authors.initials: AC
authors.lastname: Martins Junqueira
authors.forename: T A
authors.initials: TA
authors.lastname: Lemos
authors.forename: E L
authors.initials: EL
authors.lastname: Kemper
authors.forename: F R
authors.initials: FR
authors.lastname: da Silva
authors.forename: A L
authors.initials: AL
authors.lastname: Vettore
authors.forename: P
authors.initials: P
authors.lastname: Arruda
authors.forename: A M
authors.initials: AM
authors.lastname: Azeredo-Espin
mesh_terms: D000818:Animals; D001482:Base Composition; D003062:Codon; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics*; D004175:Diptera / Q000145:classification / Q000235:genetics*; D017344:Genes, Insect; D016678:Genome; D019476:Insect Proteins / Q000235:genetics; D008928:Mitochondria / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA
chemical_list: D003062:Codon; D004272:DNA, Mitochondrial; D019476:Insect Proteins