Detection of circulating lipopolysaccharide-bound monocytes in children with gram-negative sepsis.
The possibility that gram-negative sepsis can be diagnosed by detection of lipopolysaccharide (LPS) bound on the surface of monocytes in the circulation of patients with gram-negative sepsis was investigated. Peripheral monocytes were analyzed by flow cytometer and an anti-LPS monoclonal antibody in 3 groups: children with gram-negative sepsis, children with gram-positive sepsis, and healthy children. LPS-bound monocytes were found in all patients with gram-negative sepsis but not in children with gram-positive sepsis or in healthy children. Therefore, the flow cytometry method developed for this study may be a novel method for diagnosing gram-negative sepsis.
Takeshita
S
S
Department of Pediatrics, National Defense Medical College, Tokorozawa, Saitama 359-8513, Japan.
Nakatani
K
K
Tsujimoto
H
H
Kawamura
Y
Y
Sekine
I
I
D000328:Adult; D016470:Bacteremia / Q000097:blood*; D016905:Gram-Negative Bacterial Infections / Q000097:blood*; D006801:Humans; D007223:Infant; D018950:Lipopolysaccharide Receptors / Q000097:blood; D008070:Lipopolysaccharides / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism*
D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides