[Detection of overexpression of insulin receptor gene in laryngeal carcinoma cells by using differential display method].
BACKGROUND
The insulin receptor (IR) is an essential protein localized on the surface of almost all cell types. IR belongs to the tyrosine-kinase growth factor receptor family. Insulin mediates proliferative responses in a variety of tumor cells, however, the role of the IR molecule in carcinogenesis has not yet exactly been established.


METHODS
Messenger RNA from mucosal keratinocytes and laryngeal carcinoma cells were transcribed in cDNA using reverse transcriptase and amplified by PCR by means of a number of oligonucleotides. PCR products were analyzed electrophoretically.


RESULTS
Comparing the electrophoretic pattern of both cell types the overexpression of a 127 bp fragment could be detected in laryngeal carcinoma cells in contrast to benign keratinocytes. Cloning and sequencing this fragment exact homologous match was found with exon-2 of the IR gene. The overexpression of the IR gene in laryngeal carcinoma cells was confirmed by Northern hybridization.


CONCLUSIONS
The results show up-regulation of IR-mRNA in laryngeal carcinoma cells suggesting that the number of IR is enhanced in these cells. Hence it follows, that the overexpression of IR plays a possible role in laryngeal cancer initiation and/or progression.
Maune
S
S
Klinik für Hals-, Nasen-, Ohrenheilkunde, Kopf- und Halschirurgie, Christian-Albrechts-Universität zu Kiel. maune@hno.uni-kiel.de
Görögh
T
T
D001345:Autoradiography; D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D005784:Gene Amplification; D015870:Gene Expression; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis; D011972:Receptor, Insulin / Q000235:genetics*; D014407:Tumor Cells, Cultured / Q000378:metabolism
D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011972:Receptor, Insulin