title: Removal of parvovirus B19 from hemoglobin solution by nanofiltration.
abstract: Parvovirus B19 (B19), which may contaminate red cell components for blood transfusion, is known to be resistant to several viral inactivation methods. To increase the safety of hemoglobin solutions as a source of red cell substitutes, we investigated the removal of parvovirus B19 from hemoglobin solution using nanofiltration. The hemoglobin solution spiked with parvovirus B19 was tangentially filtered using the BMM-35 filter (mean pore size of 35 nm) followed by BMM-15. The parvovirus B19 of 10(8.5) PT50 (median PCR titer)/10 microL was not changed after the BMM-35 filtration. However, the BMM-15 filtration decreased the parvovirus B19 from 10(8.3-8.7) PT50 to 10(1.3-2.2) PT50, indicating more than 6 log10 reduction. When the initial parvovirus B19 of 10(6.0) PT50 was subjected to the BMM-15 filtration, the residual virus was 10(-0.3-0.5) PT50 orundetected in some fraction of the filtrate. Hemoglobin recovery was 70.4 +/- 3.4%. The ratio of methemoglobin was not changed during the filtration. These findings indicate that the BMM-15 filtration is a promising approach to prepare a safer hemoglobin solution for red cell substitutes.
authors.affiliation: Hokkaido Red Cross Blood Center, Sapporo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Abe
authors.forename: H
authors.initials: H
authors.lastname: Sugawara
authors.forename: J
authors.initials: J
authors.lastname: Hirayama
authors.forename: H
authors.initials: H
authors.lastname: Ihara
authors.forename: T
authors.initials: T
authors.lastname: Kato
authors.forename: H
authors.initials: H
authors.lastname: Ikeda
authors.forename: K
authors.initials: K
authors.lastname: Ikebuchi
mesh_terms: D001802:Blood Substitutes / Q000737:chemistry; D003257:Consumer Product Safety; D004279:DNA, Viral / Q000032:analysis; D004340:Drug Contamination / Q000517:prevention & control; D006454:Hemoglobins / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D008706:Methemoglobin / Q000378:metabolism; D010108:Oxyhemoglobins / Q000378:metabolism; D016732:Parvovirus B19, Human / Q000235:genetics; D012996:Solutions; D014462:Ultrafiltration / Q000295:instrumentation / Q000379:methods*
chemical_list: D001802:Blood Substitutes; D004279:DNA, Viral; D006454:Hemoglobins; D010108:Oxyhemoglobins; D012996:Solutions; D008706:Methemoglobin