Inner ear and lateral line expression of a zebrafish Nkx5-1 gene and its downregulation in the ears of FGF8 mutant, ace.
An orthologue of the mouse homeobox gene Nkx5-1 was cloned and characterized in the zebrafish. As in the mouse and chick, the zebrafish Nkx5-1 gene is expressed in the ear placode and vesicle and in cells forming the vestibulo-acoustic ganglion. In addition, a novel expression domain, the lateral line, appears in the zebrafish, supporting a common precursor hypothesis for these two organs. In the FGF8 zebrafish mutant ace, expression of Nkx5-1 in the otic structures is diminished. The most significant reduction of zfNkx5-1 expression was observed in cells of the vestibulo-acoustic ganglion.
Adamska
M
M
Department of Molecular and Cellular Biology, Technical University, Braunschweig, Spielmannstrasse 7, D-38106, Braunschweig, Germany.
Léger
S
S
Brand
M
M
Hadrys
T
T
Braun
T
T
Bober
E
E
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000196:embryology / Q000378:metabolism; D018076:DNA, Complementary; D015536:Down-Regulation; D007758:Ear, Inner / Q000196:embryology / Q000378:metabolism*; D051524:Fibroblast Growth Factor 8; D005346:Fibroblast Growth Factors / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D008465:Mechanoreceptors / Q000196:embryology / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D051381:Rats; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D015027:Zebrafish
D018076:DNA, Complementary; C086187:Hmx3 protein, mouse; D018398:Homeodomain Proteins; D009419:Nerve Tissue Proteins; D051524:Fibroblast Growth Factor 8; D005346:Fibroblast Growth Factors