title: Expression profiling of human sulfotransferase and sulfatase gene superfamilies in epithelial tissues and cultured cells.
abstract: The bioavailability of drugs administered topically or orally depends on their metabolism by epithelial enzymes such as the cytosolic sulfotransferases (SULT). Reverse transcriptase-polymerase chain reaction (RT-PCR) methods were established to detect expression of 8 SULT genes and 4 arylsulfatase (ARS) genes in human tissues of epithelial origin and in cultures of normal and transformed (cancer) cells. The results indicate: (i) SULT 1A1, 1A3, ARSC, and ARSD genes are ubiquitously expressed; (ii) expression is frequently similar between cell lines and corresponding tissues; (iii) SULT gene expression in normal cultured cells is generally comparable to the expression in associated transformed (cancer) cell lines; (iv) SULT 1A1 promoter usage is mainly tissue specific; however, both promoters are frequently used in SULT 1A3 expression; and (v) the expression profile of SULT 1A1, 1A3, 1E1, and 2B1a/b suggests that one or more of these isoforms may be involved in the cutaneous sulfoconjugation of minoxidil and cholesterol.
authors.affiliation: IntegriDerm, Inc., 2130 Memorial Parkway, South West, Huntsville, Alabama, 35801, USA. dooley@integriderm.com
authors.forename: T P
authors.initials: TP
authors.lastname: Dooley
authors.forename: R
authors.initials: R
authors.lastname: Haldeman-Cahill
authors.forename: J
authors.initials: J
authors.lastname: Joiner
authors.forename: T W
authors.initials: TW
authors.lastname: Wilborn
mesh_terms: D001682:Biological Availability; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D002478:Cells, Cultured; D002784:Cholesterol / Q000378:metabolism; D003593:Cytoplasm / Q000201:enzymology / Q000378:metabolism; D017931:DNA Primers; D004847:Epithelial Cells / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism; D004848:Epithelium / Q000201:enzymology* / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D008297:Male; D008914:Minoxidil / Q000378:metabolism; D009363:Neoplasm Proteins / Q000235:genetics; D009928:Organ Specificity; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013429:Sulfatases / Q000235:genetics*; D015238:Sulfotransferases / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: D017931:DNA Primers; D007527:Isoenzymes; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D008914:Minoxidil; D002784:Cholesterol; D015238:Sulfotransferases; D013429:Sulfatases