Enantiomeric separation and quantitative determination of atenolol in tablets by chiral high-performance liquid chromatography.
The separation and quantitative determination of atenolol isomers by chiral high-performance liquid chromatography (HPLC) are described. Atenolol isomers were separated using a Chiralcel OD column (250 x 4.6 mm, 10 microns); the mobile phase was hexane-ethanol-diethylamine (75:25:0.1 v/v/v); ultraviolet detection was at 276 nm; and flow rate was 0.7 ml/min. The coefficient of variation and average recovery of (R)-isomer were 0.60% and 100.37%, respectively, for sample A and 0.69% and 100.63%, respectively, for sample B. The coefficient of variation and average recovery of (S)-isomer were 0.59% and 100.33%, respectively, for sample A and 0.63% and 99.78%, respectively, for sample B.
Santoro
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MI
Departamento de Farmácia, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Brasil.
Cho
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Kedor-Hackmann
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D000319:Adrenergic beta-Antagonists / Q000737:chemistry / Q000493:pharmacokinetics*; D001262:Atenolol / Q000737:chemistry / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007536:Isomerism; D013607:Tablets
D000319:Adrenergic beta-Antagonists; D013607:Tablets; D001262:Atenolol