Identification of novel metabolites in the degradation of phenanthrene by Sphingomonas sp. strain P2.
Sphingomonas sp. strain P2, which is capable of utilizing phenanthrene as a sole carbon and energy source, was isolated from petroleum-contaminated soil in Thailand. Gas chromatography-mass spectrometry and (1)H and (13)C nuclear magnetic resonance analyses revealed two novel metabolites from the phenanthrene degradation pathway. One was identified as 5,6-benzocoumarin, which was derived by dioxygenation at the 1- and 2-positions of phenanthrene, and the other was determined to be 1,5-dihydroxy-2-naphthoic acid. Other metabolites from phenanthrene degradation were identified as 7, 8-benzocoumarin, 1-hydroxy-2-naphthoic acid and coumarin. From these results, it is suggested that strain P2 can degrade phenanthrene via dioxygenation at both 1,2- and 3,4-positions followed by meta-cleavage.
Pinyakong
O
O
Biotechnology Research Center, The University of Tokyo, Japan.
Habe
H
H
Supaka
N
N
Pinpanichkarn
P
P
Juntongjin
K
K
Yoshida
T
T
Furihata
K
K
Nojiri
H
H
Yamane
H
H
Omori
T
T
D001673:Biodegradation, Environmental; D003374:Coumarins; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy; D010616:Phenanthrenes / Q000378:metabolism*; D020578:Sphingomonas / Q000254:growth & development / Q000378:metabolism*
D003374:Coumarins; D010616:Phenanthrenes; C031181:phenanthrene