Identification of a novel human mitochondrial D-loop RNA species which exhibits upregulated expression following cellular immortalization.
We report the identification and characterization of a novel human mitochondrial RNA species approximately 0.47 kb long that is transcribed from the mtDNA L-strand and is derived from the D-loop. Its expression increases when human cells become immortal, a key event in tumorigenesis. The RNA is therefore designated IDL (Immortalization-associated D-Loop). Sequence and hybrid cell analyses suggest that the increased level of IDL RNA in immortal cells is due to a recessive change, possibly in the activity of a trans-acting factor that controls IDL RNA expression.
Duncan
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Children's Medical Research Institute, 214 Hawkesbury Road, Westmead, Sydney, New South Wales, 2145, Australia.
Perrem
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Reddel
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D001483:Base Sequence; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000032:analysis / Q000235:genetics*; D005786:Gene Expression Regulation; D006801:Humans; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012313:RNA / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D000077278:RNA, Mitochondrial; D015534:Trans-Activators / Q000502:physiology; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D015854:Up-Regulation
D004272:DNA, Mitochondrial; D000077278:RNA, Mitochondrial; D015534:Trans-Activators; D012313:RNA