title: The Egr-1 promoter contains information for constitutive and inducible expression in transgenic mice.
abstract: Egr-1 is an immediate early gene that couples short-term changes in the extracellular milieu to long-term changes in gene expression. Under in vitro conditions, the Egr-1 gene is expressed in many cell types and is induced by a wide variety of extracellular signals. The mechanisms by which the Egr-1 gene is regulated in vivo remain poorly understood. In this study, we have generated transgenic mice with a construct containing 1200 bp of the mouse Egr-1 promoter coupled to nuclear localized LacZ. In multiple independent lines of mice, reporter gene expression was detected in subsets of endothelial cells, vascular smooth-muscle cells, cardiomyocytes, neurons, and hepatocytes. This pattern closely resembled that of the endogenous gene. After partial hepatectomy, reporter gene activity was upregulated between two- and fivefold in regenerating livers. Taken together, these findings suggest that the Egr-1 promoter contains information for appropriate spatial and temporal expression in vivo.
authors.affiliation: Department of Molecular Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02215, USA.
authors.forename: J C
authors.initials: JC
authors.lastname: Tsai
authors.forename: L
authors.initials: L
authors.lastname: Liu
authors.forename: B C
authors.initials: BC
authors.lastname: Cooley
authors.forename: M R
authors.initials: MR
authors.lastname: DiChiara
authors.forename: J N
authors.initials: JN
authors.lastname: Topper
authors.forename: W C
authors.initials: WC
authors.lastname: Aird
mesh_terms: D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics*; D051766:Early Growth Response Protein 1; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D006498:Hepatectomy; D017874:Immediate-Early Proteins / Q000235:genetics*; D007763:Lac Operon; D051379:Mice; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic; D014018:Tissue Distribution; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D051766:Early Growth Response Protein 1; C486109:Egr1 protein, mouse; D017874:Immediate-Early Proteins; D014157:Transcription Factors