title: Molecular characterisation of emergent multiresistant Salmonella enterica serotype [4,5,12:i:-] organisms causing human salmonellosis.
abstract: Salmonella multidrug-resistant clinical organisms identified as serotype [4,5,12:i:-] were typed using selected genetic procedures and compared with typhimurium organisms collected in the same Spanish region. Results showed a low genetic heterogeneity among [4,5,12:i:-] organisms, which generated identical ribotypes and similar but not identical XbaI PFGE, RAPD, and plasmid profiles. Multidrug resistance could be eliminated by curing and seems to be mediated by 140-kb (spvC+) and 120-kb (spvC-) non-self-transferable plasmids. The [4,5,12:i:-] organisms fall into a single genetic lineage, which emerged in 1997 and presents a different degree of genetic relationship with typhimurium lineages.
authors.affiliation: Departamento de Biología Funcional, Area Microbiología, Facultad de Medicina, Universidad de Oviedo, Spain.
authors.forename: B
authors.initials: B
authors.lastname: Guerra
authors.forename: I
authors.initials: I
authors.lastname: Laconcha
authors.forename: S M
authors.initials: SM
authors.lastname: Soto
authors.forename: M A
authors.initials: MA
authors.lastname: González-Hevia
authors.forename: M C
authors.initials: MC
authors.lastname: Mendoza
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015373:Bacterial Typing Techniques; D004352:Drug Resistance, Microbial; D018432:Drug Resistance, Multiple; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D021521:Ribotyping; D012480:Salmonella Infections / Q000382:microbiology*; D019779:Salmonella enterica / Q000145:classification / Q000187:drug effects* / Q000235:genetics* / Q000472:pathogenicity; D012486:Salmonella typhimurium / Q000145:classification / Q000235:genetics; D012703:Serotyping; D014774:Virulence
chemical_list: D000900:Anti-Bacterial Agents