Induction by soluble factors of organized axial structures in chick epiblasts.
Inductive action of soluble factors was tested on isolated chick epiblasts. An assay was developed wherein conditioned medium derived from the Xenopus XTC cell line induced the formation of a full-length notochord and rows of bilaterally symmetric somites. Basic fibroblast growth factor, epidermal growth factor, retinoic acid, and transforming growth factor type B1 and B2 were not capable of inducing axial structures. Thus, soluble factors can elicit the development of polarity stored in the epiblast and behave as true morphogens since they can induce the formation of the organized complex structures that constitute the embryonic axis.
Mitrani
E
E
Zoology Department, the Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Shimoni
Y
Y
D000818:Animals; D001756:Blastoderm / Q000187:drug effects / Q000648:ultrastructure; D002642:Chick Embryo / Q000187:drug effects / Q000196:embryology*; D017077:Culture Media, Conditioned / Q000494:pharmacology; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology; D014981:Xenopus
D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; D016212:Transforming Growth Factor beta; D016222:Fibroblast Growth Factor 2; D014212:Tretinoin; D004815:Epidermal Growth Factor