CFU-S(11) activity does not localize solely with the aorta in the aorta-gonad-mesonephros region.
The aorta-gonad-mesonephros (AGM) region is a potent hematopoietic site in the midgestation mouse conceptus and first contains colony-forming units-spleen day 11 (CFU-S(11)) at embryonic day 10 (E10). Because CFU-S(11) activity is present in the AGM region before the onset of hematopoietic stem cell (HSC) activity, CFU-S(11) activity in the complex developing vascular and urogenital regions of the AGM was localized. From E10 onward, CFU-S(11) activity is associated with the aortic vasculature, and is found also in the urogenital ridges (UGRs). Together with data obtained from organ explant cultures, in which up to a 16-fold increase in CFU-S(11) activity was observed, it was determined that CFU-S(11) can be increased autonomously both in vascular sites and in UGRs. Furthermore, CFU-S(11) activity is present in vitelline and umbilical vessels. This, together with the presence of CFU-S(11) in the UGRs 2 days before HSC activity, suggests both temporally and spatially distinct emergent sources of CFU-S(11). (Blood. 2000;96:2902-2904)
de Bruijn
M F
MF
Department of Cell Biology and Genetics, Erasmus University, Rotterdam, The Netherlands.
Peeters
M C
MC
Luteijn
T
T
Visser
P
P
Speck
N A
NA
Dzierzak
E
E
D000818:Animals; D021461:Animals, Outbred Strains; D001011:Aorta / Q000196:embryology*; D019070:Cell Lineage; D003433:Crosses, Genetic; D005260:Female; D005865:Gestational Age; D006066:Gonads / Q000196:embryology*; D006411:Hematopoiesis, Extramedullary; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D008297:Male; D008650:Mesonephros; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008855:Microscopy, Electron, Scanning; D011828:Radiation Chimera