title: Neoadjuvant, hyperfractionated irradiation induces apoptosis and decreases proliferation in squamous cell cancer of the oral cavity.
abstract: Twenty-eight patients with squamous cell carcinoma of the oral cavity were treated with a total dose of 20 Gy. Tissue samples for immunohistochemistry were taken at the time of diagnostic biopsy and at surgery after radiotherapy (RX). For detection of proliferating cells, the immunoperoxidase reaction with Ki67 was performed. Apoptotic cells were detected by the TdT-mediated biotin dUTP nick end labeling (TUNEL) method. RX reduced proliferation in 27 patients, only in one case did the proliferation index (PI) increase. Delta PI (PI before RX PI following RX) amounted to 4.11% (SD=3.2%; P<0.0001). The apoptotic index (AI) increased significantly subsequent to neoadjuvant RX. Delta AI (AI after RX--AI before RX) measured 1.82% (SD=0.9; P<0.001). These data indicate that RX of patients suffering from squamous cell carcinoma of the oral cavity with a dosis of 20 Gy induces apoptosis and inhibits proliferation of tumor cells.
authors.affiliation: Dept. of Internal Medicine, Knappschaftskrankenhaus, University of Bochum, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Petrasch
authors.forename: A
authors.initials: A
authors.lastname: Reinacher-Schick
authors.forename: B
authors.initials: B
authors.lastname: Busemann
authors.forename: J
authors.initials: J
authors.lastname: Radtke
authors.forename: S
authors.initials: S
authors.lastname: Philippou
authors.forename: T
authors.initials: T
authors.lastname: Dörr
authors.forename: G
authors.initials: G
authors.lastname: Kemmeries
authors.forename: H
authors.initials: H
authors.lastname: Eufinger
authors.forename: W
authors.initials: W
authors.lastname: Schmiegel
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis / Q000528:radiation effects*; D002294:Carcinoma, Squamous Cell / Q000532:radiotherapy* / Q000601:surgery; D002455:Cell Division / Q000528:radiation effects; D053938:DNA Fragmentation; D004307:Dose-Response Relationship, Radiation; D004847:Epithelial Cells / Q000528:radiation effects; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D019394:Ki-67 Antigen / Q000032:analysis; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000532:radiotherapy* / Q000601:surgery; D011184:Postoperative Period; D011879:Radiotherapy Dosage; D018714:Radiotherapy, Adjuvant; D018709:Statistics, Nonparametric; D016896:Treatment Outcome
chemical_list: D019394:Ki-67 Antigen