Direct analysis of single rat peritoneal mast cells with laser vaporization/ionization mass spectrometry.
A linear time-of-flight mass spectrometer was used as a detector for flow cytometry. These two techniques were coupled by a laser vaporization/ionization interface. The estimated mass detection limit of the combined system was 20 amol of serotonin standard with one laser pulse. An aqueous buffer at physiological pH was used to ensure compatibility with cells. Rat peritoneal mast cells (RPMCs) were dispensed into the mass spectrometer in a single file confined within a 20-micron-i.d. capillary. By using the mass spectrometer as a detector, no precolumn staining or derivatization is required. Determination of serotonin and histamine in individual cells was demonstrated. With this method, hundreds of cells can be analyzed within a few minutes. The average amounts of histamine and serotonin per RPMC were found to be 0.75 +/- 0.33 and 0.11 +/- 0.06 fmol, respectively. No correlation was found between the amounts of the two amines in each cell.
Fung
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Ames Laboratory-USDOE and Department of Chemistry, Iowa State University 50011, USA.
Yeung
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D000818:Animals; D002470:Cell Survival; D005434:Flow Cytometry / Q000379:methods; D006632:Histamine / Q000032:analysis; D007834:Lasers; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D008407:Mast Cells / Q000378:metabolism; D010529:Peritoneal Cavity / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000032:analysis
D012701:Serotonin; D006632:Histamine