A new class of microtubule-associated proteins in plants.
In plants there are three microtubule arrays involved in cellular morphogenesis that have no equivalent in animal cells. In animals, microtubules are decorated by another class of proteins - the structural MAPS - which serve to stabilize microtubules and assist in their organization. The best-studied members of this class in plants are the MAP-65 proteins that can be purified together with plant microtubules after several cycles of polymerization and depolymerization. Here we identify three similar MAP-65 complementary DNAs representing a small gene family named NtMAP65-1, which encode a new set of proteins, collectively called NtMAP65-1. We show that NtMAP65-1 protein localizes to areas of overlapping microtubules, indicating that it may function in the behaviour of antiparallel microtubules in the mitotic spindle and the cytokinetic phragmoplast.
Smertenko
A
A
School of Biological Sciences, Royal Holloway University of London, Egham, Surrey, TW20 OEX, UK.
Saleh
N
N
Igarashi
H
H
Mori
H
H
Hauser-Hahn
I
I
Jiang
C J
CJ
Sonobe
S
S
Lloyd
C W
CW
Hussey
P J
PJ
D000595:Amino Acid Sequence; D002451:Cell Compartmentation; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D005455:Fluorescent Antibody Technique; D008869:Microtubule-Associated Proteins / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics* / Q000302:isolation & purification; D010947:Plants, Toxic; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000235:genetics*
D018076:DNA, Complementary; D018744:DNA, Plant; D008869:Microtubule-Associated Proteins; D010940:Plant Proteins