Stabilization of P450 2B4 by its association with P450 1A2 revealed by high-pressure spectroscopy.
We studied the effect of intermolecular interactions between cytochromes P450 1A2 (CYP1A2) and 2B4 (CYP2B4) on the barotropic inactivation of the ferrous carbonyl complexes of the hemoproteins. When taken separately, these hemoproteins reveal quite distinct barotropic behavior. While the 2B4(Fe(2+))-CO complex is very sensitive to hydrostatic pressures and undergoes P450 --> P420 transition at rather low pressures (P(1/2) = 297 MPa, DeltaV(0) = -61 ml/mol), the 1A2(Fe(2+))-CO is extremely resistant to barotropic inactivation. Only about 8% of the 1A2 was exposed to pressure-induced P450 --> P420 transition (P(1/2) = 420 MPa, DeltaV(0) = -28 ml/mol). The formation of the mixed oligomers of 2B4 and 1A2 was found to have a dramatic effect on the barotropic behavior of 2B4. In the heterooligomers of 1A2 and 2B4, the 2B4 hemoprotein appears to be largely protected from barotropic inactivation. In 1:1 mixed oligomers no more than 25% of the total P450 content undergoes P450 --> P420 inactivation with the molar reaction volume value (DeltaV(0) = -26 ml/mol) similar to those found for pure 1A2. Moreover, interactions between 1A2 and 2B4 results in a displacement of the Soret band of the ferrous carbonyl complex of CYP2B4 to shorter wavelength (from 451.3 to 448.4 nm) and largely strengthens the dependence of the Soret band wavenumber on hydrostatic pressure below 200 MPa. This effect suggests an important hydration of the CYP2B4 heme moiety in response to the interactions with CYP1A2. We discuss these results in terms of the hypothesis that the heterooligomerization of cytochromes P450 in microsomes plays an important role in the control of the activity and coupling of the microsomal monooxygenase.
Davydov
D R
DR
Institut of Biomedical Chemistry, Russian Academy of Medical Science, 10 Pogodinskaya, Moscow, 119832, Russia.
Petushkova
N A
NA
Archakov
A I
AI
Hoa
G H
GH
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases; D019388:Cytochrome P-450 CYP1A2 / Q000737:chemistry / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry* / Q000378:metabolism*; D004789:Enzyme Activation; D004795:Enzyme Stability; D005296:Ferrous Compounds / Q000378:metabolism; D006418:Heme / Q000737:chemistry / Q000378:metabolism; D006874:Hydrostatic Pressure; D007501:Iron / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D011485:Protein Binding; D020836:Protein Structure, Quaternary; D011817:Rabbits; D013057:Spectrum Analysis; D013250:Steroid Hydroxylases / Q000737:chemistry* / Q000378:metabolism*; D013314:Stress, Mechanical; D013816:Thermodynamics; D014867:Water / Q000378:metabolism
D005296:Ferrous Compounds; D014867:Water; D006418:Heme; D003577:Cytochrome P-450 Enzyme System; D007501:Iron; D013250:Steroid Hydroxylases; D001189:Aryl Hydrocarbon Hydroxylases; D019388:Cytochrome P-450 CYP1A2; C050356:steroid 15-alpha-hydroxylase