Salmonella enteritidis temperature-sensitive mutants protect mice against challenge with virulent Salmonella strains of different serotypes.
The protection conferred by temperature-sensitive mutants of Salmonella enteritidis against different wild-type Salmonella serotypes was investigated. Oral immunization with the single temperature-sensitive mutant E/1/3 or with a temperature-sensitive thymine-requiring double mutant (E/1/3T) conferred: (i) significant protection against the homologous wild-type Salmonella strains; (ii) significant cross-protection toward high challenge doses of S. typhimurium. Significant antibody levels against homologous lipopolysaccharide and against homologous and heterologous protein antigens were detected in sera from immunized mice. Moreover, a wide range of protein antigens from different Salmonella O serotypes were recognized by sera from immunized animals. Besides, primed lymphocytes from E/1/3 immunized mice recognized Salmonella antigens from different serotypes. Taken together, these results indicate that temperature-sensitive mutants of S. enteritidis are good candidates for the construction of live vaccines against Salmonella.
Gherardi
M M
MM
Departamento de Microbiología Aplicada, Centro de Estudios Farmacológicos y Botánicos (CEFYBO-CONICET), Serrano 669, 1414 Buenos Aires, Argentina.
Gómez
M I
MI
García
V E
VE
Sordelli
D O
DO
Cerquetti
M C
MC
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000942:Antigens, Bacterial / Q000032:analysis; D015153:Blotting, Western; D008070:Lipopolysaccharides / Q000032:analysis; D008213:Lymphocyte Activation; D051379:Mice; D009154:Mutation; D012481:Salmonella Infections, Animal / Q000276:immunology / Q000517:prevention & control*; D022562:Salmonella Vaccines / Q000627:therapeutic use*; D012477:Salmonella enteritidis / Q000235:genetics / Q000276:immunology*; D013696:Temperature; D014613:Vaccines, Attenuated / Q000627:therapeutic use
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D008070:Lipopolysaccharides; D022562:Salmonella Vaccines; D014613:Vaccines, Attenuated