Conditioned fear-induced changes in neuropeptide Y-like immunoreactivity in rats: the effect of diazepam and buspirone.
The study aimed to investigate the influence of conditioned fear, produced in the passive avoidance test, on neuropeptide Y-like immunoreactivity (NPY-LI) and the effect of anxiolytics on NPY-LI in frightened rats. Rats avoided the dark chamber, where they were previously subjected to electric footshock, and they exhibited increased numbers of defecations and gastric ulcers. Moreover, they showed increased NPY-LI in the amygdala, nucleus accumbens and hypothalamus, and decreased NPY-LI in the frontal cortex. Diazepam (1 or 3 mg/kg) and buspirone (1.5 or 5 mg/kg) dose-dependently inhibited passive avoidance and decreased the numbers of defecations, and they also decreased the number of gastric ulcers. Diazepam reversed while buspirone only attenuated the fear-induced changes in NPY-LI in all regions studied. In the amygdala, the effect of diazepam was dose-dependent. The effect of diazepam on both behaviour and NPY-LI was antagonized by flumazenil (15 mg/kg). Apart from supporting the role of the NPY system in fear and anxiety, the results of this study suggest that NPY is involved in the anxiolytic effects of diazepam and buspirone and that the effect of diazepam is mediated by benzodiazepine receptors.
Krysiak
R
R
Department of Clinical Pharmacology, Medical University of Silesia, Medyków 18, 40-752 Katowice, Poland. rmkrysiak@rocketmail.com
Obuchowicz
E
E
Herman
Z S
ZS
D000679:Amygdala / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002065:Buspirone / Q000494:pharmacology*; D003213:Conditioning, Psychological; D003672:Defecation; D003975:Diazepam / Q000494:pharmacology*; D004597:Electroshock; D005239:Fear / Q000187:drug effects / Q000502:physiology*; D005442:Flumazenil / Q000494:pharmacology; D005625:Frontal Lobe / Q000378:metabolism; D007031:Hypothalamus / Q000378:metabolism; D008297:Male; D008568:Memory; D009478:Neuropeptide Y / Q000378:metabolism*; D009714:Nucleus Accumbens / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013276:Stomach Ulcer / Q000209:etiology
D009478:Neuropeptide Y; D005442:Flumazenil; D003975:Diazepam; D002065:Buspirone