Complement activation by PVA as measured by ELIFA (enzyme-linked immunoflow assay) for SC5b-9.
Polyvinyl alcohol (PVA) coated onto polyethylene (PE) tubes exposed to human serum for 1 hour at 37 degrees C resulted in the production of 1.03 +/- 0.04 microg/cm2 of the soluble form of the terminal membrane attack complex, SC5b-9. This was approximately 20 x that produced by the polyethylene. About one quarter of this total was found associated with the surface of PVA. SC5b-9 concentrations were determined by enzyme-linked immunoflow assay (ELIFA) a variant on ELISA that involved drawing the test sample, the antibodies and the chromogenic reagent through a nitrocellulose membrane filter. ELIFA enabled analysis of protein concentrations in the presence of SDS, so that SDS (0.05%) was used to desorb adsorbed SC5b-9 prior to analysis together with SC5b-9 in the bulk to get a more complete picture of PVA-associated complement activation.
Black
J P
JP
Department of Chemical Engineering and Applied Chemistry, Institute of Biomaterials and Biomedical Engineering, University of Toronto, ON, Canada.
Sefton
M V
MV
D001672:Biocompatible Materials; D002138:Calibration; D003167:Complement Activation / Q000187:drug effects*; D015938:Complement Membrane Attack Complex / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007124:Immunoenzyme Techniques; D011095:Polyethylenes / Q000494:pharmacology; D011142:Polyvinyl Alcohol / Q000494:pharmacology*; D012826:Silicone Elastomers / Q000494:pharmacology
D001672:Biocompatible Materials; D015938:Complement Membrane Attack Complex; D011095:Polyethylenes; D012826:Silicone Elastomers; D011142:Polyvinyl Alcohol