title: The crystal structure of 3alpha -hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni shows a novel oligomerization pattern within the short chain dehydrogenase/reductase family.
abstract: The crystal structure of 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni (3alpha-HSDH) as well as the structure of its binary complex with NAD(+) have been solved at 1.68-A and 1.95-A resolution, respectively. The enzyme is a member of the short chain dehydrogenase/reductase (SDR) family. Accordingly, the active center and the conformation of the bound nucleotide cofactor closely resemble those of other SDRs. The crystal structure reveals one homodimer per asymmetric unit representing the physiologically active unity. Dimerization takes place via an interface essentially built-up by helix alphaG and strand betaG of each subunit. So far this type of intermolecular contact has exclusively been observed in homotetrameric SDRs but never in the structure of a homodimeric SDR. The formation of a tetramer is blocked in 3alpha-HSDH by the presence of a predominantly alpha-helical subdomain which is missing in all other SDRs of known structure.
authors.affiliation: Institut für Molekularbiologie und Tumorforschung, Philipps-Universität Marburg, 35037 Marburg, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Grimm
authors.forename: E
authors.initials: E
authors.lastname: Maser
authors.forename: E
authors.initials: E
authors.lastname: Möbus
authors.forename: G
authors.initials: G
authors.lastname: Klebe
authors.forename: K
authors.initials: K
authors.lastname: Reuter
authors.forename: R
authors.initials: R
authors.lastname: Ficner
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000737:chemistry*; D045002:3-alpha-Hydroxysteroid Dehydrogenase (B-Specific); D000429:Alcohol Oxidoreductases / Q000737:chemistry*; D000595:Amino Acid Sequence; D001665:Binding Sites; D020134:Catalytic Domain; D003460:Crystallization; D008969:Molecular Sequence Data; D009243:NAD / Q000378:metabolism; D017510:Protein Folding
chemical_list: D009243:NAD; D015096:3-Hydroxysteroid Dehydrogenases; D000429:Alcohol Oxidoreductases; D045002:3-alpha-Hydroxysteroid Dehydrogenase (B-Specific)