Immunization of dogs with a canine herpesvirus vector expressing Neospora caninum surface protein, NcSRS2.
In order to develop a vaccine against Neospora caninum in dogs, we constructed recombinant canine herpesvirus (CHV) expressing N. caninum surface protein, NcSRS2. Indirect immunofluorescence indicated that the antigenic structure of the recombinant NcSRS2 was similar to the authentic parasite protein. The dogs immunised with recombinant virus produced IgG antibody to N. caninum, and their sera recognised the parasite protein on Western blot. The dogs inoculated with recombinant virus showed no clinical symptoms and infectious CHV was not recovered from the dogs, suggesting that recombinant CHV expressing N. caninum proteins may lead to a vaccine against neosporosis in dogs.
Nishikawa
Y
Y
The National Research Center for Protozoan Diseases, Obihiro University, Inada-cho, Obihiro, Hokkaido, 080-8555, Japan.
Ikeda
H
H
Fukumoto
S
S
Xuan
X
X
Nagasawa
H
H
Otsuka
H
H
Mikami
T
T
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000032:analysis / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology; D015153:Blotting, Western / Q000662:veterinary; D003048:Coccidiosis / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D017931:DNA Primers / Q000737:chemistry; D004283:Dog Diseases / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control*; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D005822:Genetic Vectors / Q000737:chemistry; D002184:Herpesvirus 1, Canid / Q000276:immunology*; D007114:Immunization / Q000662:veterinary; D008297:Male; D018688:Neospora / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015800:Protozoan Proteins / Q000276:immunology*; D016052:Protozoan Vaccines / Q000276:immunology / Q000592:standards; D014614:Vaccines, Synthetic / Q000276:immunology / Q000592:standards
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D017931:DNA Primers; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; C115389:SRS2 protein, Neospora caninum; D014614:Vaccines, Synthetic