title: Thyroglobulin is selected as luminal protein cargo for apical transport via detergent-resistant membranes in epithelial cells.
abstract: Thyroid hormone synthesis by thyrocytes depends upon apical secretion of thyroglobulin (Tg), the glycoprotein prohormone. In stably transfected MDCK cells, recombinant Tg is also secreted apically. All secreted Tg has undergone Golgi carbohydrate modification, whereas most intracellular Tg (which is slow to exit the endoplasmic reticulum) is sensitive to digestion with endoglycosidase H. However, in MDCK cells and PC Cl3 thyrocytes, a subpopulation of newly synthesized recombinant and endogenous Tg, respectively, is recovered in a Triton X-100 insoluble, glycosphingolipid/cholesterol-enriched (GEM/raft) fraction, and this small subpopulation is overwhelmingly endoglycosidase H resistant. Upon apical secretion, Tg solubility is restored. Apical secretion of Tg is inhibited by cellular cholesterol depletion. In FRT cells, recombinant Tg becomes Triton X-100 insoluble within 60 min after synthesis and a portion is actually endoglycosidase H-sensitive, suggesting early Tg entry into GEMs/rafts. Interestingly in FRT cells, Tg remains associated with the apical plasma membrane upon exocytosis, and all surface Tg is GEM/raft-associated. Thus, Tg is the first secretory protein demonstrated to enter Triton X-100 insoluble membranes en route to the apical surface of epithelial cells. The data imply that Tg utilizes a cargo-selective mechanism for apical sorting.
authors.affiliation: Centro de Biologia Molecular "Severo Ochoa," Universidad Autonoma de Madrid, Madrid 280-49, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Martin-Belmonte
authors.forename: M A
authors.initials: MA
authors.lastname: Alonso
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: P
authors.initials: P
authors.lastname: Arvan
mesh_terms: D000818:Animals; D001692:Biological Transport; D019921:Biotinylation; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D002784:Cholesterol / Q000378:metabolism; D004285:Dogs; D004847:Epithelial Cells / Q000378:metabolism; D006028:Glycosphingolipids / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D017830:Octoxynol / Q000494:pharmacology; D013954:Thyroglobulin / Q000378:metabolism*
chemical_list: D006028:Glycosphingolipids; D017830:Octoxynol; D013954:Thyroglobulin; D002784:Cholesterol