title: Neurotrophic factors influence upregulation of constitutive isoform of heme oxygenase and cellular stress response in the spinal cord following trauma. An experimental study using immunohistochemistry in the rat.
abstract: The influence of brain derived neurotrophic factor (BDNF) or insulin like growth factor-1 (IGF-1) on spinal cord trauma induced carbon monoxide (CO) production and cellular stress response was examined using immunostaining of the constitutive isoform of the hemeoxygenase (HO-2) enzyme and the heat shock protein (HSP 72kD) expression in a rat model. Subjection of rats to a 5 h spinal trauma inflicted by an incision into the right dorsal horn at T10-11 segment markedly upregulated the HO-2 and HSP expression in the adjacent spinal cord segments (T9 and T12). Pretreatment with BDNF or IGF-1 significantly attenuated the trauma induced HSP expression. The upregulation of HO-2 was also considerably reduced. These results show that BDNF and IGF-1 attenuate cellular stress response and production of CO following spinal cord injury which seems to be the key factors in neurotrophins induced neuroprotection.
authors.affiliation: Department of Medical Cell Biology, Biomedical Centre, Uppsala University, Sweden. Sharma@medcellbiol.uu.se
authors.forename: H S
authors.initials: HS
authors.lastname: Sharma
authors.forename: F
authors.initials: F
authors.lastname: Nyberg
authors.forename: T
authors.initials: T
authors.lastname: Gordh
authors.forename: P
authors.initials: P
authors.lastname: Alm
authors.forename: J
authors.initials: J
authors.lastname: Westman
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology*; D007150:Immunohistochemistry; D008297:Male; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000201:enzymology* / Q000473:pathology*; D015854:Up-Regulation
chemical_list: D019208:Brain-Derived Neurotrophic Factor