title: Duplication of genes encoding non-clathrin coat protein gamma-COP in vertebrate, insect and plant evolution.
abstract: Coatomer is a major component of COPI vesicles and consists of seven subunits. The gamma-COP subunit of the coatomer is believed to mediate the binding to the cytoplasmic dilysine motifs of membrane proteins. We characterized cDNAs for Copg genes encoding gamma-COP from mouse, zebrafish, Drosophila melanogaster and Bombyx mori. Two copies of Copg genes are present in vertebrates and in B. mori. Phylogenetic analysis revealed that two paralogous genes had been derived from a single ancestral gene by duplication independently in vertebrates and in B. mori. Mouse Copg1 showed ubiquitous expression with the highest level in testis. Zebrafish copg2 was biallelically expressed in hybrid larvae in contrast to its mammalian ortholog expressed in a parent-of-origin-specific manner. A phylogenetic analysis with partial plant cDNA sequences suggested that copg gene was also duplicated in the grass family (Poaceae).
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejon, South Korea.
authors.forename: Y
authors.initials: Y
authors.lastname: Hahn
authors.forename: Y J
authors.initials: YJ
authors.lastname: Lee
authors.forename: J H
authors.initials: JH
authors.lastname: Yun
authors.forename: S K
authors.initials: SK
authors.lastname: Yang
authors.forename: C W
authors.initials: CW
authors.lastname: Park
authors.forename: K
authors.initials: K
authors.lastname: Mita
authors.forename: T L
authors.initials: TL
authors.lastname: Huh
authors.forename: M
authors.initials: M
authors.lastname: Rhee
authors.forename: J H
authors.initials: JH
authors.lastname: Chung
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D012831:Bombyx / Q000235:genetics; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics*; D020756:Coatomer Protein; D004331:Drosophila melanogaster / Q000235:genetics; D019143:Evolution, Molecular; D020440:Gene Duplication; D006801:Humans; D007313:Insecta; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D010944:Plants; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013737:Testis / Q000378:metabolism; D014714:Vertebrates; D015027:Zebrafish / Q000235:genetics
chemical_list: D002352:Carrier Proteins; D020756:Coatomer Protein