The use of the 2-aminobenzoic acid tag for oligosaccharide gel electrophoresis.
Gel electrophoresis of fluorophore labeled saccharides provides a rapid and reliable method to screen enzymatic and/or chemical treatments of polysaccharides and glycoconjugates, as well as a sensitive and efficient microscale method to separate and purify oligosaccharides for further analysis. A simple and inexpensive method of derivatization and analysis using 2-aminobenzoic acid (anthranilic acid, AA) is described and applied to the extracellular polysaccharide released by the desiccation tolerant cyanobacterium Nostoc commune DRH-1. The results of these analyses suggest a possible protective functionality of two pendent groups, as well as a potential relationship between these groups and the desiccation tolerance of the organism.
Huang
Z
Z
Fralin Biotechnology Center, Virginia Tech, Blacksburg 24061, USA.
Prickett
T
T
Potts
M
M
Helm
R F
RF
D002240:Carbohydrate Sequence; D000458:Cyanobacteria / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005456:Fluorescent Dyes; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000302:isolation & purification*; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D062367:ortho-Aminobenzoates / Q000737:chemistry*
D005456:Fluorescent Dyes; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; D062367:ortho-Aminobenzoates; C031385:anthranilic acid