Involvement of membrane surface charge in thermal stability of the rat ovarian LH/hCG receptor.
Analysis of fluorescence of membrane-bound 8-anilino-1-naphthalene sulfonate and monodansylcadaverine probes revealed that a negative membrane surface charge derived from free fatty acids (FFA) resulted in destabilization of structure-functional properties of the rat ovarian LH/hCG receptor. Removal of FFA from rat luteal and porcine ovarian granulosa cells by BSA increased gonadotropin responsiveness of cells in cAMP formation.
Scsuková
S
S
Institute of Experimental Endocrinology, Slovak Academy of Sciences, Vlárska 3, 833 06, Bratislava, Slovak Republic.
Jezová
M
M
Vranová
J
J
Kolena
J
J
D000817:Anilino Naphthalenesulfonates; D000818:Animals; D002103:Cadaverine / Q000031:analogs & derivatives*; D002462:Cell Membrane / Q000737:chemistry*; D000242:Cyclic AMP / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000737:chemistry; D005260:Female; D006107:Granulosa Cells / Q000737:chemistry* / Q000378:metabolism; D006358:Hot Temperature; D008184:Luteal Cells / Q000737:chemistry / Q000378:metabolism; D010053:Ovary / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D011974:Receptors, LH / Q000737:chemistry*; D012710:Serum Albumin, Bovine; D013050:Spectrometry, Fluorescence; D055672:Static Electricity; D013329:Structure-Activity Relationship; D013552:Swine
D000817:Anilino Naphthalenesulfonates; D005230:Fatty Acids, Nonesterified; D011974:Receptors, LH; D012710:Serum Albumin, Bovine; C027132:1-anilino-8-naphthalenesulfonate; D000242:Cyclic AMP; C008542:monodansylcadaverine; D002103:Cadaverine