title: Thermally labile domains in the collagen molecule.
abstract: We have proposed that the denaturation kinetics of the characteristic sharp melting point of the collagen molecules is an irreversible rate rather than an equilibrium process as previously believed. This leads to the concept of domains of variable thermal stability along the length of the molecule. We have identified the major thermally labile domains from which the denaturation process is initiated as hydroxyproline deficient sequences of 65, 65 and 59 residues near the carboxy terminus in fibrillar collagen types I, II and III, respectively. These domains differ in that there is a single hydroxyproline in the type II domain and two hydroxyprolines in the type III domain. Similar sized domains are conserved in these collagen types across species including amphibians and invertebrates. The effective size of the domain is reduced in the fibrillar aggregates to 26 residues due to the interaction with adjacent molecules and because of the precise quarter-staggered alignment of the molecules the domains are located in the gap region. This spatial confinement within the lattice of the fibre leads to the significant increase in denaturation temperature of the fibre compared to the molecule. These labile domains have also been located in molecules that form the non-fibrillar type IV basement membrane collagens and the fibril-associated aggregates such as type IX. Based on the location of the different domains in type IX we have proposed a different arrangement of the type IX on the type II fibril. The model stresses the importance of hydroxyproline in stabilising the triple helix and supports the concept of hydrogen-bonded water-bridges originally proposed from X-ray diffraction studies in contrast to other studies indicating water-bridges do not play a role in stabilising the collagen molecule.
authors.affiliation: Collagen Research Group, Division of Molecular and Cellular Biology, University of Bristol Langford, BS40 5DS, Bristol, UK.
authors.forename: C A
authors.initials: CA
authors.lastname: Miles
authors.forename: A J
authors.initials: AJ
authors.lastname: Bailey
mesh_terms: D000595:Amino Acid Sequence; D002151:Calorimetry; D003094:Collagen / Q000737:chemistry* / Q000648:ultrastructure; D006801:Humans; D006860:Hydrogen Bonding; D006909:Hydroxyproline / Q000737:chemistry; D008969:Molecular Sequence Data; D011489:Protein Denaturation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D013696:Temperature
chemical_list: D003094:Collagen; D006909:Hydroxyproline