Estimation of molecular linear free energy relationship descriptors. 4. Correlation and prediction of cell permeation.
OBJECTIVE
The passage of molecules across cell membranes is a crucial step in many physiological processes. We therefore seek physical models of this process, in order to predict permeation for new molecules, and to better understand the important interactions which determine the rate of permeation.


METHODS
Several sets of cell permeation data reported by Collander have been correlated against calculated Linear Free Energy Relation (LFER) descriptors. These descriptors, taken as the sum of fragmental contributions, cover the size, polarity, polarizabilty, and hydrogen bonding capacity of each molecule.


RESULTS
For 36 values of permeation into Chara ceratophylla cells, a model (sd = 0.24) dominated by hydrogen bond acidity is found, while for 63 rates of permeation values into Nitella cells a very similar model yields sd = 0.46. Comparisons between the two cell types are made directly for 17 compounds in both data sets, indicate differences of a similar magnitude to the standard deviations of the above models. The two data sets can be combined to yield a generic model of rates of permeation into cells, resulting in an sd value of 0.46 for a total of 100 data points.


CONCLUSIONS
Models allowing accurate prediction of cell permeation have been constructed using 100 experimental data. We demonstrate that hydrogen bond acidity is the dominating factor in determining cell permeation for two distinct species of algal cell.
Platts
J A
JA
Department of Chemistry, University College London, UK.
Abraham
M H
MH
Hersey
A
A
Butina
D
D
D000465:Algorithms; D002463:Cell Membrane Permeability / Q000502:physiology*; D000460:Chlorophyta / Q000378:metabolism; D056890:Eukaryota / Q000378:metabolism; D006860:Hydrogen Bonding; D018499:Linear Energy Transfer; D008954:Models, Biological