Monoclonal antibody-based enzyme immunoassay for mercury(II) determination.
A monoclonal antibody (K3C6) was developed against Hg(II) and applied in different enzyme immunoassay (EIA) formats to determine the test system with the highest sensitivity. A detection limit of 1. 0 microg/L Hg(II) could be achieved with a competitive format in contrast to a detection limit of 2.1 microg/L Hg(II) with a noncompetitive EIA. A competitive displacement EIA yielded the best detection limit of 0.4 microg/L Hg(II) and was well suited to measuring real samples. For this purpose different water samples were diluted at least 1:10 to avoid matrix effects and subsequently spiked with 1 microg/L HgCl(2). Recovery of the spiked samples was between 80 and 120%.
Marx
A
A
Department of Botany, Technische Universität München at Weihenstephan, Alte Akademie 12, Freising, D-85350, Germany.
Hock
B
B
D000042:Absorption; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001667:Binding, Competitive / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007124:Immunoenzyme Techniques / Q000379:methods*; D008628:Mercury / Q000032:analysis* / Q000276:immunology*; D012492:Salts / Q000494:pharmacology; D012680:Sensitivity and Specificity; D014867:Water; D014876:Water Pollution / Q000032:analysis
D000911:Antibodies, Monoclonal; D012492:Salts; D014867:Water; D008628:Mercury