Antitumor activity of alpha fetoprotein and epidermal growth factor conjugates in vitro and in vivo.
Conjugates of carminomycin (Cm) with alpha-fetoprotein (AFP) and epidermal growth factor (EGF) were prepared and their cytotoxic activities were studied in vitro. Both conjugates showed cytotoxic activity which exceeded that of free Cm in tumor cell cultures of MCF-7, SKOV3, QOS, P388 and B16 cells. The antitumor effects of the conjugates were studied in vivo in mice with subcutaneous tumors of B16 and P388 cells. The Cm-AFP and Cm-EGF conjugates inhibited tumor growth and noticeably increased the mean life span in experimental animals. Our results suggest that the therapeutic activity of Cm can be significantly enhanced by conjugation to AFP or EGF.
Lutsenko
S V
SV
Center for Bioengineering, Russian Academy of Sciences, Moscow State University, Moscow, Russia.
Feldman
N B
NB
Finakova
G V
GV
Gukasova
N V
NV
Petukhov
S P
SP
Posypanova
G A
GA
Skryabin
K G
KG
Severin
S E
SE
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D002360:Carubicin / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology*; D006801:Humans; D007941:Leukemia P388 / Q000188:drug therapy / Q000473:pathology; D008546:Melanoma, Experimental / Q000188:drug therapy / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009368:Neoplasm Transplantation; D014407:Tumor Cells, Cultured / Q000187:drug effects; D000509:alpha-Fetoproteins / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*
D000903:Antibiotics, Antineoplastic; D000509:alpha-Fetoproteins; D004815:Epidermal Growth Factor; D002360:Carubicin