Human nectin3/PRR3: a novel member of the PVR/PRR/nectin family that interacts with afadin.
We have isolated nectin3/PRR3, the fourth human member of the nectin/PRR family, also described as the alpha herpes virus receptor family. Nectin/PRR members are adhesion molecules expressed at intercellular junctions. Nectin3/PRR3 is a transmembrane protein, whose extracellular region contains three Ig-like domains (V, C and C) and shares approximately 30% identity with the other members. It is mainly expressed in testis and placental tissues. SDS-PAGE analyses demonstrate that nectin3/PRR3 has a molecular weight of 83kDa. Nectin1/PRR1L and nectin2/PRR2S and L were found to be specifically expressed at the intercellular junctions. This localization is in part due to the interaction of the C-terminal part of these receptors (ended by the consensus sequence A/EXYV) and the PDZ domain of afadin. In this report we demonstrate that the nectin3/PRR3 receptor carries the A/EXYV consensus sequence and interacts in vivo with both long and short isoforms of afadin. These results suggest that the human nectin3/PRR3 is a new afadin-associated molecule.
Reymond
N
N
INSERM U.119, Unité de Cancérologie et Thérapeutique Expérimentale, 27 Bd Lei Roure, 13009, Marseille, France.
Borg
J P
JP
Lecocq
E
E
Adelaide
J
J
Campadelli-Fiume
G
G
Dubreuil
P
P
Lopez
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006801:Humans; D020014:K562 Cells; D016547:Kinesins; D008297:Male; D008840:Microfilament Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D009218:Myosins; D000075983:Nectins; D011233:Precipitin Tests; D011485:Protein Binding; D012313:RNA / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured; D020298:U937 Cells
C095516:AFDN protein, human; D015815:Cell Adhesion Molecules; D018076:DNA, Complementary; D008840:Microfilament Proteins; C000619010:NECTIN1 protein, human; C000619014:NECTIN3 protein, human; D000075983:Nectins; C108835:afadin; D012313:RNA; D009218:Myosins; D016547:Kinesins