Method to obtain endoscopic esophageal samples for primary cell culture: focus on infectious contamination.
Cell culture techniques hold great importance for the development of molecular biology. However, when used to study oncology, most of the samples come from surgical specimens. Endoscopy is a interesting alternative to get samples for culture. We studied a protocol to allow the control of infectious contamination potentially related to endoscopy, which could preclude it as a method to obtain cells for culture. Esophageal biopsies from 30 patients were taken through upper gastrointestinal endoscopy, using a previously flamed forceps, and were cultivated with and without amphotericin. Our results showed contamination in 3.3% of the wells without the antifungal and in 0.8% of those with it. Regarding the 30 cases studied, the described protocol was able to provide samples free of contamination in all of them.
Constant-Neto
M
M
Instituto de Pesquisas Biomédicas, Faculdade de Medicina/Hospital São Lucas, Pontificia Universidade Católica do Rio Grande do Sul, Porto Alegre-RS, Brasil.
Falavigna
M
M
Castro
A C
AC
Machado
D C
DC
D001706:Biopsy / Q000379:methods*; D002478:Cells, Cultured; D004945:Esophagoscopy; D004947:Esophagus / Q000166:cytology*; D006801:Humans; D013048:Specimen Handling / Q000379:methods*