title: Numerical densities of myonuclei and satellite cells in muscle fiber types in the aging human thyroarytenoid muscle: an immunohistochemical and stereological study using confocal laser scanning microscopy.
abstract: OBJECTIVE
  This study determines the role of changes in numerical densities of myonuclei and satellite cells in age-related remodeling of the thyroarytenoid muscle (TA).
  
  
  METHODS
  Changes in numerical densities (N(V)) and ratios (N(N)) of myonuclei and satellite cells were estimated for the entire TA by use of stereological techniques.
  
  
  RESULTS
  There was no age-related change or difference between fiber types for N(V myonucleus, fiber), but N(V myonucleus, fiber) increased with decreasing fiber diameter. There was a trend toward a decrease in N(V satellite cell, fiber) and a decrease in N(N satellite cell, myonucleus). N(V satellite cell, fiber) was higher for type 1 than for type 2 fibers, and type 1 satellite cells increased disproportionately with increasing total satellite cell numerical density.
  
  
  CONCLUSIONS
  Decreased satellite cell proliferation may contribute to age-related fiber loss and atrophy in the TA.
  
  
  CONCLUSIONS
  Therapeutic techniques based on activation of satellite cells may block age-related fiber loss and atrophy in the TA.
authors.affiliation: Department of Otolaryngology and Communication Sciences, SUNY Upstate Medical University, Syracuse, NY 13210, USA.
authors.forename: L T
authors.initials: LT
authors.lastname: Malmgren
authors.forename: P J
authors.initials: PJ
authors.lastname: Fisher
authors.forename: C E
authors.initials: CE
authors.lastname: Jones
authors.forename: L M
authors.initials: LM
authors.lastname: Bookman
authors.forename: T
authors.initials: T
authors.lastname: Uno
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000473:pathology*; D002466:Cell Nucleolus / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure*; D002478:Cells, Cultured; D003593:Cytoplasm / Q000648:ultrastructure; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007821:Laryngeal Muscles / Q000648:ultrastructure*; D008297:Male; D018613:Microscopy, Confocal; D008875:Middle Aged; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000648:ultrastructure*; D012680:Sensitivity and Specificity