title: Induction of apoptosis in human leukaemic cell lines K562, HL60 and U937 by diethylhexylphthalate isolated from Aloe vera Linne.
abstract: We investigated the effect of diethylhexylphthalate (DEHP) from Aloe vera Linne on the apoptosis of human leukaemic cell lines K562, HL60 and U937 to examine its pharmacological activity. At a level of 10 microg mL(-1) DEHP a significant anti-leukaemic effect was observed for all three cell lines, as measured by clonogenic assay. After treatment with 10 microg mL(-1) DEHP for 4 h, agarose gel electrophoresis and flow cytometric analysis confirmed the occurrence of apoptosis. These results indicate that DEHP isolated from Aloe vera Linne has a potent antileukaemic effect, and thus represents a new type of pharmacological activity with respect to human leukaemic cells.
authors.affiliation: Animal Resource Research Center, Konkuk University, Seoul, Korea.
authors.forename: K H
authors.initials: KH
authors.lastname: Lee
authors.forename: H S
authors.initials: HS
authors.lastname: Hong
authors.forename: C H
authors.initials: CH
authors.lastname: Lee
authors.forename: C H
authors.initials: CH
authors.lastname: Kim
mesh_terms: D000504:Aloe; D017209:Apoptosis / Q000187:drug effects*; D004051:Diethylhexyl Phthalate / Q000302:isolation & purification / Q000494:pharmacology*; D004587:Electrophoresis, Agar Gel; D005434:Flow Cytometry; D018922:HL-60 Cells / Q000187:drug effects*; D006801:Humans; D020014:K562 Cells / Q000187:drug effects*; D010946:Plants, Medicinal; D014407:Tumor Cells, Cultured; D014410:Tumor Stem Cell Assay; D020298:U937 Cells / Q000187:drug effects*
chemical_list: D004051:Diethylhexyl Phthalate