title: Temperature-dependent vitellogenin-mRNA expression in primary cultures of rainbow trout (Oncorhynchus mykiss) hepatocytes at 14 and 18 degrees C.
abstract: In order to study the influence of temperature on vitellogenin gene and estrogen receptor gene expression in primary hepatocytes from rainbow trout (Oncorhynchus mykiss), cells were exposed to 17beta-estradiol, bisphenol-A and nonylphenol for 48 and 96 hr. Induction of vitellogenin-mRNA expression was detected in a non-radioactive dot blot/RNAse protection assay and by RT-PCR. In the dot blot/RNAse protection assay, the estrogenic potentials of bisphenol-A and nonylphenol were about 10(4)- to 10(5)-fold and 10(5)-fold lower than that of 17beta-estradiol, respectively. The relative estrogenic potential did not show any difference between 14 and 18 degrees C. In contrast, at 18 degrees C, RT-PCR analysis revealed increased amounts of vitellogenin- and estrogen receptor-mRNA after 12 and 24 hr of exposure to 17beta-estradiol, if compared to 14 degrees C. Owing to increased vitellogenin gene expression at 18 degrees C, the sensitivity of primary hepatocytes to 17beta-estradiol and bisphenol-A could be increased.
authors.affiliation: Dept of Zoology I, University of Heidelberg, Im Neuenheimer Feld 230, D-69120, Heidelberg, Germany. spawlows@ix.urz.uni-heidelberg.de
authors.forename: S
authors.initials: S
authors.lastname: Pawlowski
authors.forename: M
authors.initials: M
authors.lastname: Islinger
authors.forename: A
authors.initials: A
authors.lastname: Völkl
authors.forename: T
authors.initials: T
authors.lastname: Braunbeck
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds; D002478:Cells, Cultured; D003080:Cold Temperature; D004958:Estradiol / Q000494:pharmacology; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D015151:Immunoblotting; D008297:Male; D023282:Nuclease Protection Assays; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D010636:Phenols / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis*; D011960:Receptors, Estrogen / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014819:Vitellogenins / Q000096:biosynthesis / Q000235:genetics*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004958:Estradiol; D010636:Phenols; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D014819:Vitellogenins; C025256:nonylphenol; C006780:bisphenol A; D000098826:Bisphenol A Compounds