title: Monoclonal antibody against Mycoplasma fermentans-specific aminoglycoglycerolipid.
abstract: Previously, we reported that Mycoplasma fermentans has specific antigens (phosphocholine-containing glycoglycerolipids: GGPL-I and GGPL-III) and discussed the possibility of their pathogenic role. In this paper, we report the characterization of a monoclonal antibody (MF-III-1) specific to GGPL-III (phosphocholine-containing aminoglycoglycerolipid) using methods of electron microscopy, immunofluorescence cell surface staining, laser scanning microscopy, immunoelectron microscopy, and thin-layer chromatography immunostaining. The MF-III-1 antibody specifically recognized M. fermentans attached to the surface of HTLV-I-infected human helper T-cells, and it did not cross-react with other lipids nor with human T-cell antigens. Since MF-III-1 distinguishes GGPL-III from GGPL-I, the binding site may include a serinol (2-amino-1,3-propanediol) residue of GGPL-III. MF-III-1 is useful for the in vitro study of M. fermentans, and may also be useful as a tool for the study of the involvement of M. fermentans in human diseases.
authors.affiliation: Department of Microbiology, Tokyo Medical and Dental University, Japan. kmatsuda@gan2.res.ncc.go.jp
authors.forename: K
authors.initials: K
authors.lastname: Matsuda
authors.forename: J L
authors.initials: JL
authors.lastname: Li
authors.forename: S
authors.initials: S
authors.lastname: Ichinose
authors.forename: R
authors.initials: R
authors.lastname: Harasawa
authors.forename: M
authors.initials: M
authors.lastname: Saito
authors.forename: N
authors.initials: N
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology*; D002478:Cells, Cultured; D002855:Chromatography, Thin Layer; D005260:Female; D006017:Glycolipids / Q000276:immunology*; D006801:Humans; D008055:Lipids / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D016253:Microscopy, Immunoelectron; D016858:Mycoplasma fermentans / Q000276:immunology* / Q000648:ultrastructure; D006377:T-Lymphocytes, Helper-Inducer / Q000382:microbiology
chemical_list: C106372:1''-phosphocholine-2''-aminodihydroxypropane-3''-phospho-6'-glucopyranosyl-(1'-3)-1,2-dipalmitoylglycerol; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D006017:Glycolipids; D008055:Lipids