title: Maternal cold inducible RNA binding protein is required for embryonic kidney formation in Xenopus laevis.
abstract: We cloned a major isoform of Xenopus homologue of cold inducible RNA binding protein (CIRP), XCIRP-1. XCIRP-1 was neither cold inducible nor essential for cell division during early embryonic development. Suppression of XCIRP-1 dose dependently produced tailbuds with deformations of the brain and internal organs. The defects were XCIRP-1 specific as they could be rescued by sense transcript. Suppression of XCIRP-1 also disrupted the morphogenetic migration of the C3 blastomeres (lineaged to become the embryonic kidney, the pronephros). In animal cap explants, depletion of XCIRP-1 inhibited activin/retinoic acid induced expressions of pronephros related Xlim-1 and WT1 genes. These results suggest that XCIRP-1 is required for the specification and morphogenetic lineage migration of the pronephros.
authors.affiliation: Department of Neurology, The First Military Medical University of China, Guangzhou, PR China.
authors.forename: Y
authors.initials: Y
authors.lastname: Peng
authors.forename: K H
authors.initials: KH
authors.lastname: Kok
authors.forename: R H
authors.initials: RH
authors.lastname: Xu
authors.forename: K H
authors.initials: KH
authors.lastname: Kwok
authors.forename: D
authors.initials: D
authors.lastname: Tay
authors.forename: P C
authors.initials: PC
authors.lastname: Fung
authors.forename: H F
authors.initials: HF
authors.lastname: Kung
authors.forename: M C
authors.initials: MC
authors.lastname: Lin
mesh_terms: D000015:Abnormalities, Multiple / Q000235:genetics; D000818:Animals; D002455:Cell Division; D003001:Cloning, Molecular; D004625:Embryo, Nonmammalian / Q000502:physiology; D019143:Evolution, Molecular; D005260:Female; D007668:Kidney / Q000166:cytology / Q000196:embryology*; D009024:Morphogenesis; D010641:Phenotype; D010802:Phylogeny; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013489:Suppression, Genetic; D014158:Transcription, Genetic; D029867:Xenopus Proteins; D014982:Xenopus laevis / Q000196:embryology* / Q000235:genetics
chemical_list: C491389:CIRP2 protein, Xenopus; D020033:Protein Isoforms; D016601:RNA-Binding Proteins; D029867:Xenopus Proteins