title: Increased expression of intestinal P-selectin and pulmonary E-selectin during intravenous total parenteral nutrition.
abstract: OBJECTIVE
  Intravenous total parenteral nutrition (TPN) induces intestinal polymorphonuclear neutrophil recruitment with increased intestinal intercellular adhesion molecule-1 expression. While intercellular adhesion molecule-1 causes firm adhesion of leukocytes to the endothelial cells, P- and E-selectin mediate leukocyte recruitment via rolling. Therefore, manipulation of nutrition may also affect P- and E-selectin expression in organs.
  
  
  METHODS
  Prospective randomized experimental trials.
  
  
  METHODS
  Laboratory.
  
  
  METHODS
  Male mice.
  
  
  METHODS
  Fifty-three mice were randomized to chow, intravenous TPN, or intragastric TPN.
  
  
  METHODS
  After 5 days of diet, mice were administered iodine 125-labeled anti-P-selectin antibody (or iodine 125-labeled anti-E-selectin antibody) and iodine 131-labeled nonbinding antibody to quantify P-selectin (or E-selectin) expression in organs (lung, liver, kidney, small intestine, colon, stomach, pancreas, mesentery, heart, and skeletal muscle).
  
  
  RESULTS
  P-selectin in small intestine, colon, stomach, and pancreas in the intravenous TPN group increased significantly as compared with the chow and the intragastric TPN groups. E-selectin expression was up-regulated after intravenous TPN in the lung but not in other sites.
  
  
  CONCLUSIONS
  In a time frame (5 days) when intercellular adhesion molecule-1 expression and neutrophil recruitment are increased, intestinal expression of P-selectin remains up-regulated. Early lung inflammatory changes are reflected by increases in E-selectin. This change may reflect early pulmonary dysfunction with intravenous TPN, but its significance requires further study.
authors.affiliation: Departments of Surgery, University of Tennessee, Memphis, TN, USA.
authors.forename: K
authors.initials: K
authors.lastname: Fukatsu
authors.forename: A H
authors.initials: AH
authors.lastname: Lundberg
authors.forename: M K
authors.initials: MK
authors.lastname: Hanna
authors.forename: Y
authors.initials: Y
authors.lastname: Wu
authors.forename: H G
authors.initials: HG
authors.lastname: Wilcox
authors.forename: D N
authors.initials: DN
authors.lastname: Granger
authors.forename: A O
authors.initials: AO
authors.lastname: Gaber
authors.forename: K A
authors.initials: KA
authors.lastname: Kudsk
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D004032:Diet; D007262:Infusions, Intravenous; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis / Q000096:biosynthesis*; D007420:Intestine, Large / Q000378:metabolism*; D007421:Intestine, Small / Q000378:metabolism*; D008168:Lung / Q000378:metabolism; D008297:Male; D051379:Mice; D023421:Models, Animal; D019007:P-Selectin / Q000032:analysis / Q000096:biosynthesis*; D010289:Parenteral Nutrition, Total; D011336:Probability; D011897:Random Allocation; D012016:Reference Values
chemical_list: D019007:P-Selectin; D018799:Intercellular Adhesion Molecule-1