title: Observations on the crystallization of oligogalacturonates.
abstract: Oligogalacturonates were produced by the limited enzymic hydrolysis of polygalacturonic acid and purified by ion-exchange chromatography. The fractions obtained were of limited polydispersity, determined by analytical ion-exchange chromatography. Oligomers with an average degree of polymerization of 10-15 were readily crystallized from aqueous salt solutions at neutral pH as single crystals. Crystal morphology of the salts examined, Na+, K+ and Ca2+ were characteristic of the salt. The wide-angle X-ray diffraction patterns obtained for the sodium salt were consistent with published fibre diffraction data of this salt form.
authors.affiliation: Food Quality and Materials Science, Institute of Food Research, Norwich Laboratory, Colney, UK. neil.rigby@bbsrc.ac.uk
authors.forename: N M
authors.initials: NM
authors.lastname: Rigby
authors.forename: A J
authors.initials: AJ
authors.lastname: MacDougall
authors.forename: S G
authors.initials: SG
authors.lastname: Ring
authors.forename: P
authors.initials: P
authors.lastname: Cairns
authors.forename: V J
authors.initials: VJ
authors.lastname: Morris
authors.forename: P A
authors.initials: PA
authors.lastname: Gunning
mesh_terms: D002852:Chromatography, Ion Exchange; D003461:Crystallography; D006603:Hexuronic Acids / Q000737:chemistry*; D006868:Hydrolysis; D008854:Microscopy, Electron; D009844:Oligosaccharides / Q000737:chemistry; D010368:Pectins / Q000737:chemistry; D012492:Salts / Q000494:pharmacology; D014961:X-Ray Diffraction
chemical_list: D006603:Hexuronic Acids; D009844:Oligosaccharides; D012492:Salts; C007819:galacturonic acid; D010368:Pectins