title: Antibacterial activity evaluation of microcin J25 against diarrheagenic Escherichia coli.
abstract: The inhibitory activities of known microcins were evaluated against some diarrheagenic Escherichia coli strains. Some antibacterial properties of microcin J25, the most active one, were studied. A rapid two-step purification was performed. The MIC and the minimum bactericidal concentration of J25 against E. coli O157:H7 were 1 and 100 microg ml(-1), respectively. A 10(4)-CFU ml(-1) contamination by this strain was destroyed in milk and meat extract by 6.25 microg of J25 ml(-1) and in half-diluted egg yolk by 50 microg of J25 ml(-1).
authors.affiliation: Laboratoire de Génie Protéique et Cellulaire, Pôle Sciences, Université de La Rochelle, La Rochelle, France.
authors.forename: S
authors.initials: S
authors.lastname: Sable
authors.forename: A M
authors.initials: AM
authors.lastname: Pons
authors.forename: S
authors.initials: S
authors.lastname: Gendron-Gaillard
authors.forename: G
authors.initials: G
authors.lastname: Cottenceau
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001430:Bacteriocins / Q000494:pharmacology*; D003967:Diarrhea / Q000382:microbiology*; D004926:Escherichia coli / Q000145:classification / Q000187:drug effects* / Q000302:isolation & purification; D019453:Escherichia coli O157 / Q000187:drug effects* / Q000302:isolation & purification; D006801:Humans; D008826:Microbial Sensitivity Tests; D010455:Peptides; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D010455:Peptides; D011994:Recombinant Proteins; C020411:microcin