A fruiting body tissue method for efficient Agrobacterium-mediated transformation of Agaricus bisporus.
We describe a modified Agrobacterium-mediated method for the efficient transformation of Agaricus bisporus. Salient features of this procedure include cocultivation of Agrobacterium and fruiting body gill tissue and use of a vector with a homologous promoter. This method offers new prospects for the genetic manipulation of this commercially important mushroom species.
Chen
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X
Department of Plant Pathology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.
Stone
M
M
Schlagnhaufer
C
C
Romaine
C P
CP
D000364:Agaricus / Q000235:genetics* / Q000254:growth & development; D016960:Agrobacterium tumefaciens / Q000235:genetics*; D002934:Cinnamates; D017931:DNA Primers; D004352:Drug Resistance, Microbial / Q000235:genetics; D005822:Genetic Vectors; D006921:Hygromycin B / Q000031:analogs & derivatives* / Q000494:pharmacology; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D014170:Transformation, Genetic
D002934:Cinnamates; D017931:DNA Primers; D006921:Hygromycin B; C026273:hygromycin A