[RAPD-PCR analysis of the variability of spring common wheat cultivar genomes and their androclinal double haploid form].
Based on RAPD-PCR analysis with 15 primers including those homologous to particular loci, the level of genetic polymorphism in the collection of spring common wheat genotypes and their androclinal double haploid (ADH) lines was determined. Intraspecific polymorphism of the original wheat forms was 20%. By the absence of polymorphism in the molecular patterns of ADH lines and original forms, it was shown that, under the conditions used, no genetic changes in the genomic DNA of spring common wheat in anther culture occurred. Thus, the technology of direct in vitro androgenesis does not lead to genome rearrangements and may be used for rapid production of pure lines of such a complex allopolyploid as spring common wheat.
Gerashchenkov
G A
GA
Institute of Biochemistry and Genetics, Ufa Scientific Center, Russian Academy of Sciences, Bashkortostan, Russia.
Gorbunova
V Iu
VIu
Zarianova
L D
LD
Rozhnova
N A
NA
Bakhitov
V A
VA
D001483:Base Sequence; D017931:DNA Primers; D018744:DNA, Plant; D018745:Genome, Plant; D006238:Haploidy; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D014908:Triticum / Q000187:drug effects*
D017931:DNA Primers; D018744:DNA, Plant