title: Multiparameter flow cytometry for simultaneous assessment of p53 protein expression and cellular DNA content in oral squamous cell carcinomas: evidence for the development of aneuploid clones from p53-deficient diploid progenitor cells.
abstract: Diploid tumour cells regularly continue to progress after the development of aneuploid cell populations in head and neck squamous cell carcinomas. The coexistence of aneuploid clones with their diploid progenitor cells provides a unique opportunity to study the order of appearance of p53 mutation and aneuploidy in the same tumour. Multiparameter flow cytometry was therefore applied to 22 oral squamous cell carcinomas to simultaneously assess cellular DNA content and p53 protein expression on a single-cell basis. Concurrent measurements of cytokeratin expression served to identify tumour cells of epithelial origin. One of 5 diploid and 2 of 17 aneuploid carcinomas were p53-negative. For 15 p53-positive aneuploid tumours, overexpression of p53 protein was identified for the aneuploid clones as well as for coexisting diploid tumour cell populations in 14 cases. On the understanding that coexisting diploid and aneuploid tumour cell populations have a common clonal origin, these results provide evidence that aneuploid tumour clones typically develop from p53-deficient diploid progenitor cells. Loss of wild-type p53 function may therefore contribute to the development of aneuploidy in head and neck cancer.
authors.affiliation: Division of Tumor Biology, University of Ulm, D-89070 Ulm, Germany. joerg.hemmer@medizin.uni-ulm.de
authors.forename: J
authors.initials: J
authors.lastname: Hemmer
authors.forename: J
authors.initials: J
authors.lastname: Polackova
mesh_terms: D000782:Aneuploidy; D002294:Carcinoma, Squamous Cell / Q000737:chemistry* / Q000235:genetics / Q000473:pathology; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D002999:Clone Cells / Q000473:pathology; D015331:Cohort Studies; D004273:DNA, Neoplasm / Q000032:analysis* / Q000235:genetics; D004171:Diploidy; D018450:Disease Progression; D005434:Flow Cytometry; D016158:Genes, p53; D006801:Humans; D007633:Keratins / Q000032:analysis; D009062:Mouth Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000473:pathology; D009363:Neoplasm Proteins / Q000032:analysis* / Q000096:biosynthesis; D014411:Neoplastic Stem Cells / Q000473:pathology*; D010610:Pharyngeal Neoplasms / Q000737:chemistry / Q000473:pathology; D016159:Tumor Suppressor Protein p53 / Q000032:analysis* / Q000096:biosynthesis
chemical_list: D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D016159:Tumor Suppressor Protein p53; D007633:Keratins