title: The FMRFamide-like neuropeptide AF2 is present in the parasitic nematode Haemonchus contortus.
abstract: Peptides belonging to the FMRFamide family are widely distributed amongst invertebrates. We report here on the isolation of the FMRFamide neuropeptide AF2 (Lys-His-Glu-Tyr-Leu-Arg-Phe-NH2) from the parasitic nematode Haemonchus contortus. Immunocytochemical techniques showed that FMRFamide-like material was distributed in several regions of these organisms including nerve cords and cell bodies of the central nervous system. AF2 was isolated using a method that employed 6 steps of reverse-phase HPLC. The concentration of AF2 in this organism was approximately 30 pmol/g of nematode.
authors.affiliation: Department of Physiology and Pharmacology, University of Southampton, Bassett Crescent East.
authors.forename: C D
authors.initials: CD
authors.lastname: Keating
authors.forename: L
authors.initials: L
authors.lastname: Holden-Dye
authors.forename: M C
authors.initials: MC
authors.lastname: Thorndyke
authors.forename: R G
authors.initials: RG
authors.lastname: Williams
authors.forename: A
authors.initials: A
authors.lastname: Mallett
authors.forename: R J
authors.initials: RJ
authors.lastname: Walker
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D001667:Binding, Competitive; D006189:Haemonchus / Q000737:chemistry*; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D009420:Nervous System / Q000737:chemistry*; D009479:Neuropeptides / Q000276:immunology / Q000302:isolation & purification*; D017421:Sequence Analysis
chemical_list: D009479:Neuropeptides; C073964:AF2 neuropeptide