title: Optical sensor-based oxygen tension measurements correspond with hypoxia marker binding in three human tumor xenograft lines.
abstract: Hypoxia has a negative effect on the outcome of radiotherapy and surgery and is also related to an increased incidence of distant metastasis. In this study, tumor pO(2) measurements using a newly developed time-resolved luminescence-based optical sensor (OxyLitetrade mark) were compared with bioreductive hypoxia marker binding (pimonidazole). Single pO(2) measurements per tumor were compared to hypoxia marker binding in tissue sections using image analysis. Both assays were performed in the same tumors of three human tumor lines grown as xenografts. Both assays demonstrated statistically significant differences in the oxygenation status of the three tumor lines. There was also a good correlation between hypoxia marker binding and the pO(2) measurements with the OxyLitetrade mark device. A limitation of the OxyLitetrade mark system is that it is not yet suited for sampling multiple sites in one tumor. An important strength is that continuous measurements can be taken at the same position and dynamic information on the oxygenation status of tumors can be obtained. The high spatial resolution of the hypoxia marker binding method can complement the limitations of the OxyLitetrade mark system. In the future, a bioreductive hypoxic cell marker for global assessment of tumor hypoxia may be combined with analysis of temporal changes in pO(2) with the OxyLitetrade mark to study the effects of oxygenation-modifying treatment on an individual basis.
authors.affiliation: Department of Radiation Oncology, UMC St Radboud, Joint Centre for Radiation Oncology Arnhem-Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: Bussink
authors.forename: J H
authors.initials: JH
authors.lastname: Kaanders
authors.forename: A M
authors.initials: AM
authors.lastname: Strik
authors.forename: B
authors.initials: B
authors.lastname: Vojnovic
authors.forename: A J
authors.initials: AJ
authors.lastname: van Der Kogel
mesh_terms: D000818:Animals; D001665:Binding Sites; D015374:Biosensing Techniques / Q000379:methods; D015687:Cell Hypoxia; D006801:Humans; D008163:Luminescent Measurements; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009593:Nitroimidazoles / Q000378:metabolism*; D010100:Oxygen / Q000032:analysis* / Q000737:chemistry; D010313:Partial Pressure; D011838:Radiation-Sensitizing Agents / Q000378:metabolism; D013997:Time Factors; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
chemical_list: D009593:Nitroimidazoles; D011838:Radiation-Sensitizing Agents; C033815:pimonidazole; D010100:Oxygen