title: Solid-phase synthesis of bleomycin group antibiotics. Elaboration of deglycobleomycin A(5).
abstract: The solid-phase syntheses of two deglycobleomycin A(5) analogues were achieved using a commercially available polystyrene resin containing triphenylmethyl-linked spermidine. The final products were deblocked and released from the resin, analyzed, and purified by C(18) reversed phase HPLC and characterized by high-field (1)H NMR spectroscopy and mass spectrometry. The purified products relaxed supercoiled plasmid DNA in a concentration-dependent fashion and to the same extent as authentic material derived from natural BLM A(5).
authors.affiliation: Departments of Chemistry and Biology, University of Virginia, Charlottesville, Virginia 22901, USA.
authors.forename: C J
authors.initials: CJ
authors.lastname: Leitheiser
authors.forename: M J
authors.initials: MJ
authors.lastname: Rishel
authors.forename: X
authors.initials: X
authors.lastname: Wu
authors.forename: S M
authors.initials: SM
authors.lastname: Hecht
mesh_terms: D001761:Bleomycin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D004278:DNA, Superhelical / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D015394:Molecular Structure
chemical_list: D004278:DNA, Superhelical; D001761:Bleomycin; C033284:deglycobleomycin