The mitochondrial genome of the primary screwworm fly Cochliomyia hominivorax (Diptera: Calliphoridae).
The complete sequence of the mitochondrial genome of the screwworm Cochliomyia hominivorax was determined. This genome is 16,022 bp in size and corresponds to a typical Brachycera mtDNA. A Serine start codon for COI and incomplete termination codons for COII, NADH 5 and NADH 4 genes were described. The nucleotide composition of C. hominivorax mtDNA is 77% AT-rich, reflected in the predominance of AT-rich codons in protein-coding genes. Non-optimal codon usage was commonly observed in C. hominivorax mitochondrial genes. Phylogenetic analysis distributed the Acalypterate species as a monophyletic group and assembled the C. hominivorax (Calyptratae) and the Acalyptratae in a typical Brachycera cluster. The identification of diagnostic restriction sites on the sequenced mitochondrial genome and the correlation with previous RFLP analysis are discussed.
Lessinger
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Laboratório de Genética Animal,Laboratório Genoma, Instituto de Biologia, Universidade Estadual de Campinas - UNICAMP, Campinas, São Paulo, Brazil.
Martins Junqueira
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Lemos
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TA
Kemper
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da Silva
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Vettore
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Arruda
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Azeredo-Espin
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AM
D000818:Animals; D001482:Base Composition; D003062:Codon; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics*; D004175:Diptera / Q000145:classification / Q000235:genetics*; D017344:Genes, Insect; D016678:Genome; D019476:Insect Proteins / Q000235:genetics; D008928:Mitochondria / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA
D003062:Codon; D004272:DNA, Mitochondrial; D019476:Insect Proteins