Use of a sandwich-enzyme-linked immunosorbent assay (SEA) for the diagnosis of natural Fasciola hepatica infection in cattle from Galicia (NW Spain).
An indirect-enzyme immune-linked immunosorbent assay (IEA) was compared to a sandwich-enzyme-linked immunosorbent assay (SEA) to diagnose fasciolosis in naturally infected cattle. By means of a rabbit polyclonal IgG antibody to F. hepatica antigens, captured circulating antigens were detected by an SEA technique. A total of 85.1% of the samples examined was considered positive by using the IEA-test, whereas this percentage was of 37.3% by the SEA test. Using the two enzymatic probes, only the 38.3% resulted positive to both immunoassay probes. Our data indicate that circulating antigen detection assay in combination with results of antibody detection may offer a more reliable diagnostic technique than classic coprologic ones, with results very appropriate for epidemiologic studies carried out on a wide area.
Sánchez-Andrade
R
R
Departamento de Patología Animal, Parasitología y Enfermedades Parasitarias, Facultad de Veterinaria, Universidad de Santiago de Compostela, 27071, Lugo, Spain.
Paz-Silva
A
A
Suárez
J
J
Panadero
R
R
Díez-Baños
P
P
Morrondo
P
P
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D003612:Dairying; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005211:Fascioliasis / Q000175:diagnosis / Q000662:veterinary*; D005260:Female; D007074:Immunoglobulin G; D011817:Rabbits; D013030:Spain
D007074:Immunoglobulin G