A murine monoclonal antibody produced in transgenic plants with plant-specific glycans is not immunogenic in mice.
Previous studies have shown that the production of recombinant antibodies in plants is highly efficient and presents numerous therapeutic applications. It is, however, known that plant glycoproteins display different glycosylation patterns to those exhibited by mammalian glycoproteins. Thus, it is important to know if these plant recombinant antibodies could induce undesirable immune responses in mammals; and to date no report has documented the potential immunogenicity of parenterally administered plant recombinant antibodies in animals. In order to answer this question, mice were immunised subcutaneously with a recombinant mouse monoclonal antibody produced in tobacco plants, together with alum as adjuvant. Two control groups were immunised in the same way with either the original murine monoclonal antibody or horseradish peroxidase (a plant glycoprotein). Analyses by direct immunoassay, competition immunoassay and real-time surface plasmon resonance, showed undetectable levels of antibody directed against both the protein and the glycan part of the plant recombinant antibody. These results have a direct relevance for the application of plant recombinant proteins as therapeutic agents and vaccines in humans.
Chargelegue
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Guy's, King's and St. Thomas' School of Medicine and Dentistry, Department of Oral Medicine & Pathology, Guy's Hospital, London, UK. daniel.chargelegue@kcl.ac.uk
Vine
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van Dolleweerd
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CJ
Drake
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J K
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D000818:Animals; D000907:Antibodies, Bacterial / Q000235:genetics / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology; D015374:Biosensing Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D005260:Female; D006735:Horseradish Peroxidase / Q000378:metabolism; D007114:Immunization; D007124:Immunoenzyme Techniques; D007145:Immunoglobulin kappa-Chains / Q000276:immunology; D037102:Lectins / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D037121:Plant Lectins; D030821:Plants, Genetically Modified / Q000235:genetics / Q000276:immunology*; D010947:Plants, Toxic; D011134:Polysaccharides / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000276:immunology; D013295:Streptococcus mutans / Q000276:immunology; D014026:Nicotiana / Q000235:genetics / Q000276:immunology*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000939:Epitopes; D007145:Immunoglobulin kappa-Chains; D037102:Lectins; D037121:Plant Lectins; D011134:Polysaccharides; D011993:Recombinant Fusion Proteins; D006735:Horseradish Peroxidase