Clean Western blots of membrane proteins after yeast heterologous expression following a shortened version of the method of Perini et al.
Fuentes
J M
JM
Unité de Recherche Associée 2096 (Centre National de la Recherche Scientifique et Commissariat à l'Energie Atomique), CEA Saclay, Gif-sur-Yvette Cedex, France.
Lompré
A M
AM
Møller
J V
JV
Falson
P
P
le Maire
M
M
D000818:Animals; D015153:Blotting, Western / Q000379:methods*; D000252:Calcium-Transporting ATPases / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D015870:Gene Expression; D008623:Mercaptoethanol / Q000494:pharmacology; D051381:Rats; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D015003:Yeasts / Q000378:metabolism
D012967:Sodium Dodecyl Sulfate; D008623:Mercaptoethanol; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D000252:Calcium-Transporting ATPases