Increased expression of intestinal P-selectin and pulmonary E-selectin during intravenous total parenteral nutrition.
OBJECTIVE
Intravenous total parenteral nutrition (TPN) induces intestinal polymorphonuclear neutrophil recruitment with increased intestinal intercellular adhesion molecule-1 expression. While intercellular adhesion molecule-1 causes firm adhesion of leukocytes to the endothelial cells, P- and E-selectin mediate leukocyte recruitment via rolling. Therefore, manipulation of nutrition may also affect P- and E-selectin expression in organs.


METHODS
Prospective randomized experimental trials.


METHODS
Laboratory.


METHODS
Male mice.


METHODS
Fifty-three mice were randomized to chow, intravenous TPN, or intragastric TPN.


METHODS
After 5 days of diet, mice were administered iodine 125-labeled anti-P-selectin antibody (or iodine 125-labeled anti-E-selectin antibody) and iodine 131-labeled nonbinding antibody to quantify P-selectin (or E-selectin) expression in organs (lung, liver, kidney, small intestine, colon, stomach, pancreas, mesentery, heart, and skeletal muscle).


RESULTS
P-selectin in small intestine, colon, stomach, and pancreas in the intravenous TPN group increased significantly as compared with the chow and the intragastric TPN groups. E-selectin expression was up-regulated after intravenous TPN in the lung but not in other sites.


CONCLUSIONS
In a time frame (5 days) when intercellular adhesion molecule-1 expression and neutrophil recruitment are increased, intestinal expression of P-selectin remains up-regulated. Early lung inflammatory changes are reflected by increases in E-selectin. This change may reflect early pulmonary dysfunction with intravenous TPN, but its significance requires further study.
Fukatsu
K
K
Departments of Surgery, University of Tennessee, Memphis, TN, USA.
Lundberg
A H
AH
Hanna
M K
MK
Wu
Y
Y
Wilcox
H G
HG
Granger
D N
DN
Gaber
A O
AO
Kudsk
K A
KA
D000704:Analysis of Variance; D000818:Animals; D004032:Diet; D007262:Infusions, Intravenous; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis / Q000096:biosynthesis*; D007420:Intestine, Large / Q000378:metabolism*; D007421:Intestine, Small / Q000378:metabolism*; D008168:Lung / Q000378:metabolism; D008297:Male; D051379:Mice; D023421:Models, Animal; D019007:P-Selectin / Q000032:analysis / Q000096:biosynthesis*; D010289:Parenteral Nutrition, Total; D011336:Probability; D011897:Random Allocation; D012016:Reference Values
D019007:P-Selectin; D018799:Intercellular Adhesion Molecule-1