Cloning, sequencing and expression of the tetraheme cytochrome c(3) from Desulfovibrio gigas.
The gene encoding the tetraheme cytochrome c(3) from Desulfovibrio gigas was cloned and sequenced from a 2.7-kb EcoRI-PstI insert of D. gigas DNA. The derived amino acid sequence showed that the D. gigas cytochrome c(3) is synthesized as a precursor protein with an N-terminal signal peptide sequence of 25 residues and allowed the correction of the previous reported amino acid sequence (Matias et al. Protein Science 5 (1996) 1342-1354). Expression in D. vulgaris (Hildenborough) was possible by conjugal transfer of a recombinant broad-host-range vector pSUP104 containing a SmaI fragment of the D. gigas cytochrome c(3) gene. Biochemical, immunological and spectroscopic analysis of the purified protein showed that the recombinant cytochrome is identical to that isolated from D. gigas.
da Costa
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PN
Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Apartado, 127, 2780-156 Oeiras, Potugal.
Marujo
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van Dongen
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Arraiano
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CM
Saraiva
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LM
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003574:Cytochrome c Group / Q000096:biosynthesis / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis; D003901:Desulfovibrio / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D017386:Sequence Homology, Amino Acid; D013431:Sulfates / Q000378:metabolism
D003574:Cytochrome c Group; D004269:DNA, Bacterial; D013431:Sulfates; C017165:cytochrome c(3)