title: Is a second urine specimen necessary for the diagnosis of asymptomatic bacteriuria?
abstract: By use of pulse-field gel electrophoresis, we evaluated the molecular identity of 32 Escherichia coli isolates obtained in 2 consecutive urine cultures from 16 patients as part of a large study of asymptomatic bacteriuria in diabetic women and found different E. coli isolates in 7 of 16 patients, meaning that nearly half (44%) of the patients who had been previously classified as having asymptomatic bacteriuria were reinfected with a different strain.
authors.affiliation: Department of Internal Medicine, Division of Infectious Diseases and AIDS, University Hospital Utrecht, Utrecht, The Netherlands.
authors.forename: S E
authors.initials: SE
authors.lastname: Geerlings
authors.forename: E C
authors.initials: EC
authors.lastname: Brouwer
authors.forename: W
authors.initials: W
authors.lastname: Gaastra
authors.forename: A I
authors.initials: AI
authors.lastname: Hoepelman
mesh_terms: D001437:Bacteriuria / Q000175:diagnosis* / Q000382:microbiology / Q000652:urine*; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006801:Humans
chemical_list: D004269:DNA, Bacterial