Specific detection of Plesiomonas shigelloides isolated from aquatic environments, animals and human diarrhoeal cases by PCR based on 23S rRNA gene.
Twenty-five strains of Plesiomonas shigelloides isolated from aquatic environment, 10 strains from human cases of diarrhoea and five strains from animals were identified by the polymerase chain reaction technique based on 23S rRNA gene. For this purpose, two primers targeted against part of the 5' half of the 23S rRNA gene of P. shigelloides (Escherichia coli number C-912, G-1195; Plesiomonas number C-906, G-1189) were designed. Results from our study indicated that this method might serve as a tool for a rapid and sensitive identification of P. shigelloides from different environmental and clinical sources.
González-Rey
C
C
Department of Veterinary Microbiology, SLU, Biomedical Center, Uppsala, Sweden.
Svenson
S B
SB
Bravo
L
L
Rosinsky
J
J
Ciznar
I
I
Krovacek
K
K
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D003967:Diarrhea / Q000382:microbiology*; D005618:Fresh Water / Q000382:microbiology*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology; D006801:Humans; D016981:Plesiomonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012338:RNA, Ribosomal, 23S / Q000235:genetics*; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D012703:Serotyping; D014871:Water Microbiology
D017931:DNA Primers; D012338:RNA, Ribosomal, 23S