title: The SepOvotropin: a new ovarian peptide regulating oocyte transport in Sepia officinalis.
abstract: In the cuttlefish Sepia officinalis, the successive steps of egg laying are controlled by multiple neuropeptides. Recent experiments led us to suppose that there was possible involvement of a second regulation pathway by the release of ovarian regulatory peptides in the genital tract. Using HPLC fractionation and an in vitro biological test, a C-terminal amidated peptide modulating the motility of the Sepia officinalis oviduct was isolated from an extract of vitellogenic ovarian follicles. The mass of this peptide as determined by MALDI-TOF (1501.8 Da) and analysis by Edman degradation led to the following sequence: Pro-Lys-Asp-Ser-Met-Leu-Leu-Leu-Gln-Val-Pro-Val-Tyr-amide. The peptide mapping performed by LC/MS revealed a distribution restricted to the follicles, the full grown oocytes and the eggs. This new peptide, called SepOvotropin, modulated contractions of the whole genital tract in physiological conditions from a threshold concentration between 10(-20) and 10(-19) M, demonstrating for the first time the occurrence of a specific peptidergic control of egg-laying in cephalopods.
authors.affiliation: Laboratoire de Biologie et Biotechnologies Marines, Université de Caen, Caen cedex, 14032, France.
authors.forename: C
authors.initials: C
authors.lastname: Zatylny
authors.forename: J
authors.initials: J
authors.lastname: Gagnon
authors.forename: E
authors.initials: E
authors.lastname: Boucaud-Camou
authors.forename: J
authors.initials: J
authors.lastname: Henry
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005260:Female; D008970:Molecular Weight; D008974:Mollusca / Q000737:chemistry* / Q000187:drug effects / Q000502:physiology*; D009119:Muscle Contraction / Q000187:drug effects; D009865:Oocytes / Q000737:chemistry / Q000187:drug effects* / Q000502:physiology; D006080:Ovarian Follicle / Q000737:chemistry* / Q000187:drug effects / Q000502:physiology; D010057:Oviducts / Q000187:drug effects / Q000502:physiology; D010058:Oviposition / Q000187:drug effects*; D019899:Paracrine Communication; D010449:Peptide Mapping; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010675:Pheromones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D012623:Seawater; D012680:Sensitivity and Specificity; D020539:Sequence Analysis, Protein; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D010455:Peptides; D010675:Pheromones; C415865:SepOvotropin