title: Activation of vanadium nitrogenase expression in Azotobacter vinelandii DJ54 revertant in the presence of molybdenum.
abstract: Azotobacter vinelandii carries three different and genetically distinct nitrogenase systems on its chromosome. Expression of all three nitrogenases is repressed by high concentrations of fixed nitrogen. Expression of individual nitrogenase systems is under the control of specific metal availability. We have isolated a novel type of A. vinelandii DJ54 revertant, designated A. vinelandii BG54, which carries a defined deletion in the nifH gene and is capable of diazotrophic growth in the presence of molybdenum. Inactivation of nifDK has no effect on growth of this mutant strain in nitrogen-free medium suggesting that products of the nif system are not involved in supporting diazotrophic growth of A. vinelandii BG54. Similar to the wild type, A. vinelandii BG54 is also sensitive to 1 mM tungsten. Tn5-B21 mutagenesis to inactivate the genes specific to individual systems revealed that the structural genes for vnf nitrogenase are required for diazotrophic growth of A. vinelandii BG54. Analysis of promoter activity of different nif systems revealed that the vnf promoter is activated in A. vinelandii BG54 in the presence of molybdenum. Based on these data we conclude that A. vinelandii BG54 strain utilizes vnf nitrogenase proteins to support its diazotrophic growth.
authors.affiliation: Department of Biological Sciences, Bowling Green State University, Bowling Green, OH 43403, USA.
authors.forename: S
authors.initials: S
authors.lastname: Lei
authors.forename: L
authors.initials: L
authors.lastname: Pulakat
authors.forename: N
authors.initials: N
authors.lastname: Gavini
mesh_terms: D016948:Azotobacter vinelandii / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D002876:Chromosomes, Bacterial / Q000235:genetics; D003434:Crossing Over, Genetic; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005838:Genotype; D008982:Molybdenum / Q000494:pharmacology*; D009591:Nitrogenase / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D011995:Recombination, Genetic; D015183:Restriction Mapping; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D011993:Recombinant Fusion Proteins; D008982:Molybdenum; D009591:Nitrogenase; D001616:beta-Galactosidase