A Lactobacillus helveticus plasmid detects restriction fragment length polymorphism in different bacterial species.
A small cryptic Lactobacillus helveticus plasmid, pLBL4, was able to reveal restriction fragment length polymorphism in different bacterial species including Lactobacillus species, Bacillus species, and Escherichia coli when used as a DNA probe. The observed polymorphism was a result of the combined hybridization of several microsatellite sequences. The 6-bp sequence (TTGTTT) was repeated 12 times, seven of which were concentrated within the region between 1791 and 1997 bp of the plasmid sequence. The polymorphic patterns generated with pLBL4 differed from those obtained with M13 DNA in the larger number of bands observed. The results presented here open the possibility of using pLBL4 as a new broad-spectrum polymorphic DNA probe for fingerprint analysis.
Gancheva
A G
AG
Institute of Microbiology, Bulgarian Academy of Sciences, Sofia.
Miteva
V I
VI
Stefanova
T T
TT
D001419:Bacteria / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D015342:DNA Probes; D007778:Lactobacillus / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010957:Plasmids / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping
D015342:DNA Probes