title: Nuclear localization of a hypoxia-inducible novel non-symbiotic hemoglobin in cultured alfalfa cells.
abstract: We have isolated a 483-bp-long full-length cDNA clone encoding a non-symbiotic hemoglobin called Mhb1, the first one found in alfalfa. This non-symbiotic hemoglobin is a single copy gene localized in linkage group 4 in diploid Medicago genome. The Mhb1 mRNA was found only in the roots of alfalfa plants. The Mhb1 gene was inducible by hypoxia and showed no induction by cold stress treatment. The Mhb1 transcript level increased at the G2/M boundary in a synchronized alfalfa cell suspension culture. The majority of Mhb1 protein was shown to be localized in the nucleus and smaller amounts were detected in the cytoplasm. A potential link to the nitric oxide signalling pathway is also discussed.
authors.affiliation: Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, Szeged.
authors.forename: C
authors.initials: C
authors.lastname: Seregélyes
authors.forename: L
authors.initials: L
authors.lastname: Mustárdy
authors.forename: F
authors.initials: F
authors.lastname: Ayaydin
authors.forename: L
authors.initials: L
authors.lastname: Sass
authors.forename: L
authors.initials: L
authors.lastname: Kovács
authors.forename: G
authors.initials: G
authors.lastname: Endre
authors.forename: N
authors.initials: N
authors.lastname: Lukács
authors.forename: I
authors.initials: I
authors.lastname: Kovács
authors.forename: I
authors.initials: I
authors.lastname: Vass
authors.forename: G B
authors.initials: GB
authors.lastname: Kiss
authors.forename: G V
authors.initials: GV
authors.lastname: Horváth
authors.forename: D
authors.initials: D
authors.lastname: Dudits
mesh_terms: D000595:Amino Acid Sequence; D002453:Cell Cycle; D015687:Cell Hypoxia / Q000502:physiology*; D002467:Cell Nucleus / Q000502:physiology* / Q000648:ultrastructure; D002478:Cells, Cultured; D003080:Cold Temperature; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D006454:Hemoglobins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D000455:Medicago sativa / Q000166:cytology / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D010940:Plant Proteins; D018517:Plant Roots / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
chemical_list: D006454:Hemoglobins; C415205:Mhb1 protein, Medicago sativa; D009687:Nuclear Proteins; D010940:Plant Proteins; D012333:RNA, Messenger