title: Influence of glutathione metabolising enzymes in rats with gram-negative sepsis.
abstract: OBJECTIVE
  To evaluate the role of glutathione metabolising enzymes in rats with Gram-negative sepsis.
  
  
  METHODS
  University hospital, Sweden.
  
  
  METHODS
  61 male Sprague Dawley rats.
  
  
  METHODS
  Animals were divided into two groups, one of which was given tert-butyl-4-hydroxyanisole (BHA), a powerful inducer of glutathione metabolising enzymes. A gelatine capsule containing bacteria and adjuvant substances was placed in the abdomen. In one group it contained only adjuvant substances, and the controls underwent sham laparotomy.
  
  
  METHODS
  Activities of glutathione metabolising enzymes, and histological effect on pulmonary tissue.
  
  
  RESULTS
  Activities of glutathione metabolising enzymes were reduced in lung tissue after the induction of sepsis. Pre-treatment with BHA increased enzyme activity and reduced the histological changes.
  
  
  CONCLUSIONS
  Glutathione metabolising enzymes may have a role in sepsis, and pre-treatment with BHA seems to prevent histological changes in pulmonary tissue in septic rats.
authors.affiliation: Department of Surgery, Malmö University Hospital, Sweden.
authors.forename: L
authors.initials: L
authors.lastname: Hansson
authors.forename: J
authors.initials: J
authors.lastname: Seidegård
authors.forename: L
authors.initials: L
authors.lastname: Johansson
authors.forename: B
authors.initials: B
authors.lastname: Jeppsson
mesh_terms: D000818:Animals; D001442:Bacteroides Infections / Q000201:enzymology*; D001441:Bacteroides fragilis; D004927:Escherichia coli Infections / Q000201:enzymology*; D005978:Glutathione / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008168:Lung / Q000201:enzymology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012128:Respiratory Distress Syndrome / Q000209:etiology
chemical_list: D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D005978:Glutathione