Absence of significant genotoxicity in lymphocytes and urine from workers exposed to moderate levels of cobalt-containing dust: a cross-sectional study.
Mortality studies have shown that, in the past, lung cancer occurred after exposure to mixtures of cobalt metal and metallic carbide particles, the main constituents of hard metals, but apparently not when exposure was to cobalt alone. The major objective of this biomonitoring study was to assess genotoxic effects as a measure for carcinogenic risk in workers from cobalt refineries and hard metal plants currently exposed to the threshold limit value/time-weighted average (TLV-TWA) for cobalt-containing dust. The study comprised three groups of workers: 35 workers exposed to cobalt dust from three refineries, 29 workers exposed to hard metal dust from two producing plants, and 35 matched control subjects recruited from the respective plants. The study design integrated complementary methodologies to assess biomarkers of effects that represent both initial DNA damage (8-hydroxydeoxyguanosine [8-OHdG] in urine and comet assay on lymphocytes) and definitive chromosome breakage/loss (micronuclei in lymphocytes). Cobalt and cotinine were determined in urine as a measure for cobalt exposure and recent smoking, respectively. No significant increase of genotoxic effects was detected in workers exposed to cobalt-containing dust as compared to controls. No difference in any genotoxicity biomarker was found between workers exposed to cobalt and hard metal dusts. Multiple regression analysis indicated that workers who smoked and were exposed to hard metal dusts had elevated 8-OHdG and micronuclei values. Because this observation is in line with a previous epidemiological study of an increased risk of dying from lung cancer in workers from the hard metal industry who smoked, it is concluded that this specific occupational group needs closer medical surveillance.
De Boeck
M
M
Vrije Universiteit Brussel, Laboratorium voor Cellulaire Genetica, Brussels, Belgium. mdboeck@vub.ac.be
Lardau
S
S
Buchet
J P
JP
Kirsch-Volders
M
M
Lison
D
D
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000395:Air Pollutants, Occupational / Q000009:adverse effects*; D003035:Cobalt / Q000009:adverse effects*; D020552:Comet Assay; D003430:Cross-Sectional Studies; D004249:DNA Damage / Q000187:drug effects*; D043382:DNA-Formamidopyrimidine Glycosylase; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000652:urine; D004391:Dust; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D015162:Micronucleus Tests; D008875:Middle Aged; D009699:N-Glycosyl Hydrolases / Q000378:metabolism; D016273:Occupational Exposure; D015203:Reproducibility of Results; D012643:Selenium / Q000097:blood; D017973:Tungsten Compounds / Q000009:adverse effects; D014556:Urine; D014810:Vitamin E / Q000097:blood
D000395:Air Pollutants, Occupational; D004391:Dust; D017973:Tungsten Compounds; C002802:tungsten carbide; D014810:Vitamin E; D003035:Cobalt; D000080242:8-Hydroxy-2'-Deoxyguanosine; D009699:N-Glycosyl Hydrolases; D043382:DNA-Formamidopyrimidine Glycosylase; D003849:Deoxyguanosine; D012643:Selenium