High level, tissue-specific expression of a modified calcitonin/calcitonin gene-related peptide promoter in a human medullary thyroid carcinoma cell line.
The efficient and high level expression of therapeutic genes in target cells is critical for effective gene therapy. We have developed a novel promoter by utilizing tandem repeats of a tissue-specific regulatory element from the calcitonin/calcitonin gene-related peptide (CT/CGRP) gene placed in close proximity to a basal promoter, thereby removing interstitial sequences. This promoter drives expression of reporter genes at much higher levels than the natural promoter while significantly improving specificity in thyroid C cells.
Messina
M
M
Department of Cancer Genetics, Kolling Institute of Medical Research, Royal North Shore Hospital, St. Leonards, NSW, Australia.
Yu
D M
DM
Learoyd
D L
DL
Both
G W
GW
Molloy
P L
PL
Robinson
B G
BG
D015740:Calcitonin Gene-Related Peptide / Q000096:biosynthesis / Q000235:genetics*; D018276:Carcinoma, Medullary / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D011401:Promoter Regions, Genetic; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured
D015740:Calcitonin Gene-Related Peptide