title: Amperometric glucose biosensor based on sol-gel organic-inorganic hybrid material.
abstract: A new type of sol-gel organic-inorganic hybrid material was developed and used for the production of biosensors. This material is composed of silica sol and a grafting copolymer of poly(vinyl alcohol) with 4-vinylpyridine. It prevents the cracking of conventional sol-gel-derived glasses and eliminates the swelling of the hydrogel. The optimum composition of the hybrid material was first examined, and then glucose oxidase was immobilized in this matrix to demonstrate its application. The characteristics of the biosensor were studied by cyclic voltammetry and chronoamperometry. The biosensor exhibited a series of good properties: high sensitivity (600 nA mmol-1 L-1), short response time (11 s) and remarkable long-term stability in storage (at least 5 months). In addition, the characteristics of the second-generation biosensor with the use of tetrathiafulvalene as a mediator were discussed.
authors.affiliation: Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Jilin, China.
authors.forename: B
authors.initials: B
authors.lastname: Wang
authors.forename: B
authors.initials: B
authors.lastname: Li
authors.forename: Q
authors.initials: Q
authors.lastname: Deng
authors.forename: S
authors.initials: S
authors.lastname: Dong
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D001786:Blood Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D006571:Heterocyclic Compounds / Q000737:chemistry; D006801:Humans; D011142:Polyvinyl Alcohol / Q000737:chemistry; D011725:Pyridines / Q000737:chemistry; D012822:Silicon Dioxide
chemical_list: D001786:Blood Glucose; D006571:Heterocyclic Compounds; D011725:Pyridines; C414959:poly(vinyl alcohol) grafting 4-vinylpyridine; D012822:Silicon Dioxide; D011142:Polyvinyl Alcohol; D005949:Glucose Oxidase; C063887:tetrathiafulvalene