Production of cellulases and xylanases by low-temperature basidiomycetes.
Three of four isolates, representing phylogenetically distinct groupings of low-temperature basidiomycetes (LTB), were capable of utilizing wheat straw, and to a lesser extent conifer wood at 15 degrees C. A cottony snow mould LTB (LRS 013) and a fruit rot LTB (LRS 241) grown on straw significantly degraded filter paper, carboxymethylcellulose (CMC), p-nitrophenyl beta-glucopyranoside (i.e., beta-glucosidases), and xylan. Enzymes produced by Coprinus psychromorbidus (LRS 067) were limited to xylanases from straw and wood and beta-glucosidases from wood. A sclerotia-forming LTB (LRS 131) exhibited poor growth on both substrates, and did not produce detectable quantities of extracellular enzymes. None of the LTB isolates tested degraded avicel. The temperature optima of CMCases and xylanases in the filtrates from the straw medium ranged from 25 degrees C to 55 degrees C, and with the exception of LRS 067, significant activity was observed at 5 degrees C. Two cellulases (25 and 31 kDa) and two xylanases (24 and 34 kDa) were observed on zymograms for LRS 013 and 241. Reduction of enzymes with 2-mercaptoethanol adversely affected their activity on zymograms, and an additional cellulase band was observed for non-reduced samples. This study indicates that LTB produce an array of cellulolytic and xylanolytic enzymes, and that some of these enzymes possess low-temperature optima which may facilitate degradation of plant fibre under low-temperature conditions.
Inglis
G D
GD
Agriculture and Agri-Food Canada Research Centre, Lethbridge, AB, Canada. dinglis@entomology.msstate.edu
Popp
A P
AP
Selinger
L B
LB
Kawchuk
L M
LM
Gaudet
D A
DA
McAllister
T A
TA
D001487:Basidiomycota / Q000201:enzymology* / Q000254:growth & development*; D002266:Carboxymethylcellulose Sodium / Q000378:metabolism; D002480:Cellulase / Q000096:biosynthesis*; D003080:Cold Temperature; D003302:Coprinus / Q000201:enzymology / Q000254:growth & development; D003470:Culture Media; D010209:Paper; D014197:Trees / Q000378:metabolism; D014908:Triticum / Q000378:metabolism; D043325:Xylan Endo-1,3-beta-Xylosidase; D014990:Xylans / Q000378:metabolism; D014995:Xylosidases / Q000096:biosynthesis*; D001617:beta-Glucosidase / Q000378:metabolism
D003470:Culture Media; D014990:Xylans; D014995:Xylosidases; D001617:beta-Glucosidase; D043325:Xylan Endo-1,3-beta-Xylosidase; D002480:Cellulase; D002266:Carboxymethylcellulose Sodium