title: Criteria for Candida albicans numerical analysis based on electrophoretic protein patterns.
abstract: Even in normalized gels, some problems with the choice and position of protein bands always have hindered the processing of electrophoretic data. We have developed a way to establish which criterion best fits the necessities in order to maximize the similarity indexes for numerical analysis. Some repetitions of a Candida albicans strain were carried out in eleven different gels. After staining, the bands were scored in numbers within ranges of +/- n values with increases of one quarter steps (+/- 0.25 kDa, +/- 0.5 kDa, +/- 0.75 kDa, +/- 1.0 kDa, +/- 1.25 kDa) that will limit the possibility of variation for the same bands that will appear on the repetitions. Using this criterion, we have determined that values scored within +/- 1.25 kDa could optimize the minimum limiting value of similarity in different dendrograms built.
authors.affiliation: Microbiology and Immunology Laboratory, Dentistry Faculty of Piracicaba, State University of Campinas (UNICAMP), Brazil. hofling@fop.unicamp.br
authors.forename: M F
authors.initials: MF
authors.lastname: Boriollo
authors.forename: E A
authors.initials: EA
authors.lastname: Rosa
authors.forename: R T
authors.initials: RT
authors.lastname: Rosa
authors.forename: J F
authors.initials: JF
authors.lastname: Höfling
mesh_terms: D002176:Candida albicans / Q000737:chemistry / Q000145:classification*; D002965:Classification / Q000379:methods*; D016001:Confidence Intervals; D003720:Densitometry / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005656:Fungal Proteins / Q000032:analysis*; D008970:Molecular Weight; D009172:Mycology / Q000379:methods*; D013045:Species Specificity
chemical_list: D005656:Fungal Proteins