New HLA-DRB1* nucleotide sequence of Italian origin: HLA-DRB1*13022.
High-resolution polymerase chain reaction using sequence-specific primer typing of the HLA-DRB1 gene of an Italian patient waiting for unrelated bone marrow transplantation revealed a new allelic variant of HLA-DRB1*13. Sequencing the exon 2 of DRB1* gene demonstrated a G-->C transition at the nucleotide 216 resulting in a silent mutation at codon 72: CGG-->CGC. The closest sequence was the HLA-DRB1*1302 and the new allele was named HLA-DRB1*13022. This variant was carried by the haplotype HLA-A*24; Cw*0702; B*39; DRB1*13022; DRB3*0301; DQA1*0102; DQB1*0604 as demonstrated by a family study.
Martinetti
M
M
Servizio di Immunoematologia e Trasfusione e Centro d'Immunologia dei Trapianti, IRCCS Policlinico S. Matteo, Pavla, Italy. m.martinetti@smatteo.pv.it
Pizzochero
C
C
Asti
M
M
Salvaneschi
L
L
D000328:Adult; D000483:Alleles; D001483:Base Sequence; D016026:Bone Marrow Transplantation / Q000276:immunology; D005091:Exons; D005192:Family Health; D005260:Female; D006684:HLA-DR Antigens / Q000235:genetics* / Q000276:immunology; D059811:HLA-DRB1 Chains; D006801:Humans; D007558:Italy; D015470:Leukemia, Myeloid, Acute / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D010375:Pedigree; D017354:Point Mutation / Q000276:immunology
D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains