title: Glycosidase mechanisms.
abstract: Insights into glycosidase mechanisms have come from X-ray crystallographic studies on complexes with substrate analogs and inhibitors, representing all the intermediate species along the reaction coordinate. Site-directed mutagenesis continues to play a significant role in understanding mechanisms, but is also proving important in generating glycosidases of modified mechanism or specificity.
authors.affiliation: University of British Columbia, 2036 Main Mall, Vancouver, British Columbia V6T 1Z1, Canada.
authors.forename: C S
authors.initials: CS
authors.lastname: Rye
authors.forename: S G
authors.initials: SG
authors.lastname: Withers
mesh_terms: D002240:Carbohydrate Sequence; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006026:Glycoside Hydrolases / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed
chemical_list: D004791:Enzyme Inhibitors; D006026:Glycoside Hydrolases