A novel proteinase inhibitor gene transiently induced by tobacco mosaic virus infection.
A gene (NgPI) encoding a novel proteinase inhibitor (PI) has been isolated from tobacco leaves. Protein encoded by the gene consists of 241 amino acid residues having a predicted molecular mass of 26.7 kDa and a calculated pI of 8.7. A predicted N-terminal signal sequence followed by a vacuolar targeting signal and a peptide conserved in the Kunitz type PIs were identified. The deduced NgPI protein has sequence homology with aspartic and cysteine protease inhibitors. The gene is present as double copies in the Nicotiana glutinosa genome. Expression of the NgPI gene is rapidly and transiently induced by tobacco mosaic virus infection at a time earlier than apparent lesions of hypersensitive responses appear on the leaves.
Park
K S
KS
Plant Cell Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Yusung, 305-600, Taejon, South Korea.
Cheong
J J
JJ
Lee
S J
SJ
Suh
M C
MC
Choi
D
D
D000595:Amino Acid Sequence; D018745:Genome, Plant; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D010947:Plants, Toxic; D011480:Protease Inhibitors; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000235:genetics* / Q000821:virology; D014027:Tobacco Mosaic Virus
D010940:Plant Proteins; D011480:Protease Inhibitors