title: Structure of an O-acetylated acidic O-specific polysaccharide of Proteus vulgaris O46.
abstract: An acidic O-specific polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Proteus vulgaris O46 and studied by chemical methods (O-deacetylation, sugar and methylation analyses, partial solvolysis) and 1H and 13C NMR spectroscopy. Solvolysis of the O-deacetylated polysaccharide with trifluoromethanesulfonic acid resulted in a alpha-D-GlcpNAc-(1 --> 3)-D-GlcA disaccharide that demonstrated the usefulness of this reagent for selective cleavage of heteropolysaccharides. The following structure for the polysaccharide was established: --> 4)-alpha-D-Glcp6Ac(1 --> 3)-beta-D-GlcpA4Ac-(1 --> 3)-alpha-D-GlcpNAc-(1 --> 3)-beta-D-GlcpA4Ac-(1 --> where the degree of O-acetylation is approximately 65% at position 6 of Glc and 80-95% at position 4 of GlcA residues.
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow.
authors.forename: A V
authors.initials: AV
authors.lastname: Perepelov
authors.forename: S N
authors.initials: SN
authors.lastname: Senchenkova
authors.forename: A
authors.initials: A
authors.lastname: Torzewska
authors.forename: B
authors.initials: B
authors.lastname: Bartodziejska
authors.forename: A S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: A
authors.initials: A
authors.lastname: Rozalski
authors.forename: Y A
authors.initials: YA
authors.lastname: Knirel
mesh_terms: D000107:Acetylation; D002240:Carbohydrate Sequence; D004187:Disaccharides / Q000737:chemistry; D008070:Lipopolysaccharides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D008698:Mesylates / Q000494:pharmacology; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000187:drug effects / Q000302:isolation & purification; D011514:Proteus vulgaris / Q000737:chemistry*; D012997:Solvents / Q000494:pharmacology
chemical_list: D004187:Disaccharides; D008070:Lipopolysaccharides; D008698:Mesylates; D011135:Polysaccharides, Bacterial; D012997:Solvents; C012077:trifluoromethanesulfonic acid