title: Molecular evolution in the hypervariable regions of fetuin: comparison between human and African green monkey fetuin.
abstract: Sequences of fetuin cDNA and its deduced amino acid residues from the African green monkey cell line Vero were found to differ by 7.3% and 12.9%, respectively, from the corresponding human sequences. Most amino acid substitutions were clustered within a small segment of the third domain (D3). Calculations of nonsynonymous and synonymous nucleotide substitution rates suggest that this small segment was mutated under positive selection. cDNAs encoding alpha1-antitrypsin, beta-actin and the sequences of intron 4 of alpha1-antitrypsin gene in human liver and Vero cells were also investigated. The results substantiated the positive selection imposed on the D3 segment.
authors.affiliation: Department of Biochemistry, Kinki University School of Medicine, Osaka-Sayama, Osaka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yoshida
authors.forename: Y
authors.initials: Y
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Yamamoto
authors.forename: K
authors.initials: K
authors.lastname: Matsuura
authors.forename: M
authors.initials: M
authors.lastname: Watanabe
authors.forename: H
authors.initials: H
authors.lastname: Sinohara
mesh_terms: D000199:Actins / Q000235:genetics; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D001483:Base Sequence; D002522:Chlorocebus aethiops; D022801:Complementarity Determining Regions / Q000737:chemistry* / Q000235:genetics; D003198:Computer Simulation; D018076:DNA, Complementary / Q000235:genetics; D019143:Evolution, Molecular; D006801:Humans; D007438:Introns; D008099:Liver / Q000201:enzymology; D008954:Models, Biological; D008969:Molecular Sequence Data; D009154:Mutation; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014709:Vero Cells / Q000737:chemistry; D000515:alpha 1-Antitrypsin / Q000235:genetics; D000509:alpha-Fetoproteins / Q000737:chemistry* / Q000235:genetics
chemical_list: D000199:Actins; D022801:Complementarity Determining Regions; D018076:DNA, Complementary; D000515:alpha 1-Antitrypsin; D000509:alpha-Fetoproteins