A comparison of Peronospora parasitica (Downy mildew) isolates from Arabidopsis thaliana and Brassica oleracea using amplified fragment length polymorphism and internal transcribed spacer 1 sequence analyses.
Amplified fragment length polymorphism (AFLP) fingerprints and internal transcribed spacer 1 (ITS1) sequences from 27 Peronospora parasitica isolates (collected from Arabidopsis thaliana or Brassica oleracea), 5 Albugo candida isolates (from the same hosts and from Capsella bursa-pastoris), and 1 Bremia lactucae isolate (from Lactuca sativa) were compared. The AFLP analysis divided the isolates into five groups that correlated with taxonomic species and, in most cases, with host origin. The only exception was a group consisting of A. candida isolates from both B. oleracea and C. bursa-pastoris. ITS1 sequence analysis divided the isolates into the same five groups, demonstrated the divergence between P. parasitica isolates from A. thaliana and B. oleracea, and, using previously published ITS1 sequences, clearly showed the relationship between A. candida isolates from different hosts.
Rehmany
A P
AP
Horticulture Research International, Wellesbourne, Warwickshire CV35 9EF, United Kingdom.
Lynn
J R
JR
Tör
M
M
Holub
E B
EB
Beynon
J L
JL
D017360:Arabidopsis / Q000382:microbiology*; D001937:Brassica / Q000382:microbiology*; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000235:genetics*; D020459:Genes, rRNA; D009868:Oomycetes / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA
D004271:DNA, Fungal