Use of combined microautoradiography and fluorescence in situ hybridization to determine carbon metabolism in mixed natural communities of uncultured bacteria from the genus Achromatium.
Combined microautoradiography and fluorescence in situ hybridization (FISH) was used to investigate carbon metabolism in uncultured bacteria from the genus Achromatium. All of the Achromatium species identified in a freshwater sediment from Rydal Water, Cumbria, United Kingdom, which were distinguishable only by FISH, assimilated both [(14)C]bicarbonate and [(14)C]acetate. This extends previous findings that Achromatium spp. present at another location could only utilize organic carbon sources. Achromatium spp., therefore, probably exhibit a range of physiologies, i.e., facultative chemolithoautotrophy, mixotrophy, and chemoorganoheterotrophy, similar to other large sulfur bacteria (e.g., Beggiatoa spp.).
Gray
N D
ND
Fossil Fuels and Environmental Geochemistry Postgraduate Institute and Centre for Molecular Ecology, University of Newcastle, Newcastle upon Tyne NE1 7RU, United Kingdom.
Howarth
R
R
Pickup
R W
RW
Jones
J G
JG
Head
I M
IM
D000085:Acetates / Q000378:metabolism*; D001345:Autoradiography / Q000379:methods; D001639:Bicarbonates / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism*; D002250:Carbon Radioisotopes; D005618:Fresh Water / Q000382:microbiology*; D019015:Geologic Sediments / Q000382:microbiology*; D006088:Gram-Negative Aerobic Bacteria / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism*; D017404:In Situ Hybridization, Fluorescence; D010802:Phylogeny
D000085:Acetates; D001639:Bicarbonates; D002250:Carbon Radioisotopes; D002244:Carbon