HER2 promoter controlled specific expression of the reporter gene in ovarian cancer cell line.
OBJECTIVE
Construct a retrovirus vector that expresses the downstream gene specifically in ovarian cancer cells so as to increase the specificity of gene therapy.


METHODS
HER2 promoter obtained by PCR was cloned into P(LXSN); mutant GFP cDNA was inserted just downstream of this promoter. Transfect SK-OV3 and MCF-7 cell lines with the new vector, P(LHGSN).


RESULTS
Three days after transfection with P(LHGSN), green fluorescence was observed in SK-OV3 cells while not in MCF-7 cells.


CONCLUSIONS
HER2 promoter can control tumor specific expression of the reporter gene in ovarian cancer cell lines. With proper modification, the vector is expected to be useful in tumor specific gene therapy of ovarian cancers.
Zheng
S
S
Department of Medical Genetics, China Medical University, Shenyang, Liaoning, 110001 P.R.China. wdn@soho.com
Wang
S
S
Ma
L
L
Sun
K
K
D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics; D010051:Ovarian Neoplasms / Q000235:genetics*; D011401:Promoter Regions, Genetic; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics*; D014407:Tumor Cells, Cultured
D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D018719:Erb-b2 Receptor Tyrosine Kinases