title: New neuropeptides containing carboxy-terminal RFamide and their receptor in mammals.
abstract: Only a few RFamide peptides have been identified in mammals, although they have been abundantly found in invertebrates. Here we report the identification of a human gene that encodes at least three RFamide-related peptides, hRFRP-1-3. Cells transfected with a seven-transmembrane-domain receptor, OT7T022, specifically respond to synthetic hRFRP-1 and hRFRP-3 but not to hRFRP-2. RFRP and OT7T022 mRNAs are expressed in particular regions of the rat hypothalamus, and intracerebroventricular administration of hRFRP-1 increases prolactin secretion in rats. Our results indicate that a variety of RFamide-related peptides may exist and function in mammals.
authors.affiliation: Pharmaceutical Discovery Research Division, Takeda Chemical Industries Ltd., 10 Wadai, Tsukuba, Ibaraki 300-4293, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Hinuma
authors.forename: Y
authors.initials: Y
authors.lastname: Shintani
authors.forename: S
authors.initials: S
authors.lastname: Fukusumi
authors.forename: N
authors.initials: N
authors.lastname: Iijima
authors.forename: Y
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: M
authors.initials: M
authors.lastname: Hosoya
authors.forename: R
authors.initials: R
authors.lastname: Fujii
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
authors.forename: K
authors.initials: K
authors.lastname: Kikuchi
authors.forename: Y
authors.initials: Y
authors.lastname: Terao
authors.forename: T
authors.initials: T
authors.lastname: Yano
authors.forename: T
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Kawamata
authors.forename: Y
authors.initials: Y
authors.lastname: Habata
authors.forename: M
authors.initials: M
authors.lastname: Asada
authors.forename: C
authors.initials: C
authors.lastname: Kitada
authors.forename: T
authors.initials: T
authors.lastname: Kurokawa
authors.forename: H
authors.initials: H
authors.lastname: Onda
authors.forename: O
authors.initials: O
authors.lastname: Nishimura
authors.forename: M
authors.initials: M
authors.lastname: Tanaka
authors.forename: Y
authors.initials: Y
authors.lastname: Ibata
authors.forename: M
authors.initials: M
authors.lastname: Fujino
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D003001:Cloning, Molecular; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D051381:Rats; D018013:Receptors, Neuropeptide / Q000235:genetics / Q000302:isolation & purification*; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D009479:Neuropeptides; C416987:RFamide peptide; D018013:Receptors, Neuropeptide; C058047:arginylphenylalaninamide