title: Role of insulin receptor substrate-2 in interleukin-9-dependent proliferation.
abstract: Interleukin-9 (IL-9) stimulation results in JAK, STAT and IRS1/2 phosphorylation. The role of IRS adaptor proteins in IL-9 signaling is not clear. We show that IL-9 induces IRS2 phosphorylation and association with phosphatidylinositol-3 kinase (PI 3-K) p85 subunit in TS1 cells and BaF/9R cells, which proliferate upon IL-9 stimulation. We observed a PI 3-K-dependent phosphorylation of protein kinase B (PKB) in TS1 cells, but not in BaF/9R, nor in other IL-9-dependent cell lines. Finally, 32D cells that were transfected with the IL-9 receptor but lack IRS expression survived in the presence of IL-9. Ectopic IRS1 expression allowed for IL-9-induced proliferation, in the absence of significant PKB phosphorylation.
authors.affiliation: Ludwig Institute for Cancer Research and Experimental Medicine Unit, Université Catholique de Louvain, Brussels, Belgium. jean-baptiste.demoulin@bru.licr.org
authors.forename: J B
authors.initials: JB
authors.lastname: Demoulin
authors.forename: L
authors.initials: L
authors.lastname: Grasso
authors.forename: J M
authors.initials: JM
authors.lastname: Atkins
authors.forename: M
authors.initials: M
authors.lastname: Stevens
authors.forename: J
authors.initials: J
authors.lastname: Louahed
authors.forename: R C
authors.initials: RC
authors.lastname: Levitt
authors.forename: N C
authors.initials: NC
authors.lastname: Nicolaides
authors.forename: J C
authors.initials: JC
authors.lastname: Renauld
mesh_terms: D000818:Animals; D002455:Cell Division / Q000502:physiology*; D002478:Cells, Cultured; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D006801:Humans; D055504:Insulin Receptor Substrate Proteins; D016906:Interleukin-9 / Q000502:physiology*; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D010750:Phosphoproteins / Q000096:biosynthesis / Q000502:physiology*; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt
chemical_list: D016326:Extracellular Matrix Proteins; D055504:Insulin Receptor Substrate Proteins; D016906:Interleukin-9; D047908:Intracellular Signaling Peptides and Proteins; D019869:Phosphatidylinositol 3-Kinases; D010750:Phosphoproteins; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D051057:Proto-Oncogene Proteins c-akt; C490607:ECM1 protein, human; C094556:Ecm1 protein, mouse; C526297:IRS2 protein, human; C526298:Irs2 protein, mouse