title: 31P-CP-MAS NMR studies on TPP+ bound to the ion-coupled multidrug transport protein EmrE.
abstract: The binding of tetraphenylphosphonium (TPP+) to EmrE, a membrane-bound, 110 residue Escherichia coli multidrug transport protein, has been observed by 31P cross-polarisation-magic-angle spinning nuclear magnetic resonance spectroscopy (CP-MAS NMR). EmrE has been reconstituted into dimyristoyl phosphatidylcholine bilayers. CP-MAS could selectively distinguish binding of TPP+ to EmrE in the fluid membrane. A population of bound ligand appears shifted 4 ppm to lower frequency compared to free ligand in solution, which suggests a rather direct and specific type of interaction of the ligand with the protein. This is also supported by the observed restricted motion of the bound ligand. The observation of another weakly bound substrate population arises from ligand binding to negatively charged residues in the protein loop regions.
authors.affiliation: Department of Biochemistry, University of Oxford, UK. glaubitz@bioch.ox.ac.uk
authors.forename: C
authors.initials: C
authors.lastname: Glaubitz
authors.forename: A
authors.initials: A
authors.lastname: Gröger
authors.forename: K
authors.initials: K
authors.lastname: Gottschalk
authors.forename: P
authors.initials: P
authors.lastname: Spooner
authors.forename: A
authors.initials: A
authors.lastname: Watts
authors.forename: S
authors.initials: S
authors.lastname: Schuldiner
authors.forename: H
authors.initials: H
authors.lastname: Kessler
mesh_terms: D000595:Amino Acid Sequence; D017920:Antiporters / Q000737:chemistry / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D029968:Escherichia coli Proteins; D007477:Ions; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009861:Onium Compounds / Q000737:chemistry / Q000378:metabolism*; D009943:Organophosphorus Compounds / Q000737:chemistry / Q000378:metabolism*; D010761:Phosphorus Radioisotopes
chemical_list: D017920:Antiporters; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D007477:Ions; D008565:Membrane Proteins; D009861:Onium Compounds; D009943:Organophosphorus Compounds; D010761:Phosphorus Radioisotopes; C075436:EmrE protein, E coli; C013289:tetraphenylphosphonium