The effect of Zn(2+) on the secondary structure of a histidine-rich fusogenic peptide and its interaction with lipid membranes.
Membrane fusion between uncharged lipid vesicles can be triggered by the peptide sequence 'B18' from the fertilization protein 'bindin', but it only proceeds efficiently in the presence of Zn(2+) ions. We studied (i) the interaction of Zn(2+) with the fusogenic peptide B18, (ii) the binding of B18 to 1-palmitoyl-2-oleoylglycero-3-phosphocholine (POPC), and (iii) the ternary system POPC/B18/Zn(2+). The complex formation of Zn(2+) with the central histidine-rich motif of B18 appears to shift the secondary structure away from a beta-sheet towards an alpha-helical conformation. Here we observe for the first time an essentially alpha-helical structure of the peptide when immersed in POPC bilayers which appears to represent its functional fusogenic state. Infrared linear dichroism suggests a peripheral, oblique insertion mode of B18, mediated by the hydrophobic patches along one side of the amphipathic peptide. Furthermore, the hydration level of the peptide is reduced, suggesting that the hydrophobic region of the bilayer is involved in the lipid/peptide interactions. The hydration capacity of the POPC/B18/Zn(2+) system is distinctly smaller than that of POPC/Zn(2+) without peptide. The accompanying decrease in the number of tightly bound water molecules per lipid can be interpreted as a reduction in the repulsive 'hydration' forces, which usually prevent the spontaneous fusion of lipid vesicles. Binding of the B18 peptide in the presence of Zn(2+) effectively renders the membrane surface more hydrophobic, thus allowing fusion to proceed.
Binder
H
H
University of Leipzig, Institute of Medical Physics, Liebigstr, 27, D-04103 Leipzig, Germany. binder@rz.uni-leipzig.de
Arnold
K
K
Ulrich
A S
AS
Zschörnig
O
O
D000595:Amino Acid Sequence; D000818:Animals; D002413:Cations, Divalent; D006639:Histidine / Q000737:chemistry; D008561:Membrane Fusion; D008563:Membrane Lipids / Q000737:chemistry*; D008567:Membranes, Artificial; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry; D011487:Protein Conformation; D017433:Protein Structure, Secondary / Q000187:drug effects; D012617:Sea Urchins; D013055:Spectrophotometry, Infrared; D013816:Thermodynamics; D015032:Zinc / Q000494:pharmacology*
D002413:Cations, Divalent; D008563:Membrane Lipids; D008567:Membranes, Artificial; D010455:Peptides; D010713:Phosphatidylcholines; D006639:Histidine; D015032:Zinc; C028694:1-palmitoyl-2-oleoylphosphatidylcholine