An enzyme-linked immunosorbent assay for the detection of antibody to avian reovirus by using protein sigma B as the coating antigen.
An enzyme-linked immunosorbent assay using the expressed protein sigma B as the coating antigen (sigma B-ELISA) for detecting antibody to avian reovirus (ARV) in chickens was developed and compared with a conventional ELISA. Both ELISA s and a serum neutralisation (SN) test were used to test the sera from experimentally vaccinated and farm chickens. The sigma B-ELISA could clearly distinguish the SN-positive and -negative sera in 38-week-old chickens. The correlation rate between SN and a sigma B-ELISA was 100 per cent (65/65), and that between SN and conventional ELISA was 84 per cent (55/65). With the sigma B-ELISA, all SN-negative sera had low absorbance values (below 0.06), and the absorbance values correlated closely with the SN titres. However, the sera which were antibody-negative by SN had various absorbance values, ranging from 0.07 to 0.39 in the conventional ELISA. Hence, the sigma B-ELISA had lower non-specific binding reactions than the conventional ELISA against sera from ARV -negative birds. Antibody against ARV could be detected by sigma B-ELISA after vaccination. Absorbance values peaked 4 weeks after vaccination at 2 weeks of age and were maintained until the birds were 27 weeks old. The results suggest that the presence of antibody against viral protein sigma B in birds may be used as a good indicator by the sigma B - ELISA for detecting immune status of a chicken flock or to detect chickens infected with ARV.
Shien
J H
JH
Department of Veterinary Medicine, National Chung Hsing University, Taichung, Taiwan 403, Republic of China.
Yin
H S
HS
Lee
L H
LH
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D015153:Blotting, Western / Q000662:veterinary; D002213:Capsid / Q000276:immunology*; D036022:Capsid Proteins; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D009500:Neutralization Tests / Q000662:veterinary; D018165:Orthoreovirus / Q000276:immunology*; D011201:Poultry Diseases / Q000821:virology; D016601:RNA-Binding Proteins; D013054:Spectrophotometry, Atomic / Q000662:veterinary; D014611:Vaccination / Q000662:veterinary
D000914:Antibodies, Viral; D036022:Capsid Proteins; D016601:RNA-Binding Proteins; C053179:sigma protein 3, Reovirus