title: Production of biologically active human granulocyte colony stimulating factor in the milk of transgenic goat.
abstract: We have developed a transgenic female goat harboring goat beta-casein promoter/human granulocyte colony stimulating factor (G-CSF) fusion gene by microinjection into fertilized one-cell goat zygotes. Human G-CSF was produced at levels of up to 50 microg/ml in transgenic goat milk. Its biological activity was equivalent to recombinant human G-CSF expressed from Chinese hamster ovary (CHO) cell when assayed using in vitro HL-60 cell proliferation. Human G-CSF from transgenic goat milk increased the total number of white blood cells in C57BL/6N mice with leucopenia induced by cyclophosphamide (CPA). The secreted human G-CSF was glycosylated although the degree of O-glycosylation was lower compared to CHO cell-derived human G-CSF.
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejon.
authors.forename: J H
authors.initials: JH
authors.lastname: Ko
authors.forename: C S
authors.initials: CS
authors.lastname: Lee
authors.forename: K H
authors.initials: KH
authors.lastname: Kim
authors.forename: M G
authors.initials: MG
authors.lastname: Pang
authors.forename: J S
authors.initials: JS
authors.lastname: Koo
authors.forename: N
authors.initials: N
authors.lastname: Fang
authors.forename: D B
authors.initials: DB
authors.lastname: Koo
authors.forename: K B
authors.initials: KB
authors.lastname: Oh
authors.forename: W S
authors.initials: WS
authors.lastname: Youn
authors.forename: G D
authors.initials: GD
authors.lastname: Zheng
authors.forename: J S
authors.initials: JS
authors.lastname: Park
authors.forename: S J
authors.initials: SJ
authors.lastname: Kim
authors.forename: Y M
authors.initials: YM
authors.lastname: Han
authors.forename: I Y
authors.initials: IY
authors.lastname: Choi
authors.forename: J
authors.initials: J
authors.lastname: Lim
authors.forename: S T
authors.initials: ST
authors.lastname: Shin
authors.forename: S W
authors.initials: SW
authors.lastname: Jin
authors.forename: K K
authors.initials: KK
authors.lastname: Lee
authors.forename: O J
authors.initials: OJ
authors.lastname: Yoo
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D015153:Blotting, Western; D002364:Caseins / Q000235:genetics; D002455:Cell Division; D003520:Cyclophosphamide / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005822:Genetic Vectors; D006041:Goats / Q000502:physiology*; D016179:Granulocyte Colony-Stimulating Factor / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008892:Milk / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D002364:Caseins; D017931:DNA Primers; D011993:Recombinant Fusion Proteins; D016179:Granulocyte Colony-Stimulating Factor; D003520:Cyclophosphamide