Mapping the distribution of 3-hydroxylipins in the Mucorales using immunofluorescence microscopy.
The distribution of endogenous 3-hydroxylipins (3-OH oxylipins) in representatives of the Mucorales was mapped using immunofluorescence microscopy. Strains of each of the following genera were examined: Absidia, Actinomucor, Cunninghamella, Mortierella (subgenus Micromucor), Mortierella (subgenus Mortierella), Mucor and Rhizomucor. Immunofluorescence microscopy was carried out using an antibody that was raised against 3R-hydroxy-5Z,8Z, 11 Z, 14Z-eicosatetraenoic acid (3R-HETE), which cross-reacts with other 3-OH oxylipins. Subsequently, the occurrence and distribution of the antibody on the various reproductive stages of each fungus was noted. In Absidia, Actinomucor, Mortierella (subgenus Micromucor), Mucor and Rhizomucor, 3-OH oxylipins were found to be associated with the columellae and/or wall of the sporangium. In the representative of Cunninghamella, the 3-OH oxylipins were associated with the single-spored sporangiola. No 3-OH oxylipins were detected in the strains representing Mortierella (subgenus Mortierella).
Strauss
T
T
Department of Microbiology and Biochemistry, University of the Orange Free State, Bloeinfontein, South Africa.
Botha
A
A
Kock
J L
JL
Paul
I
I
Smith
D P
DP
Linke
D
D
Schewe
T
T
Nigam
S
S
D005227:Fatty Acids / Q000302:isolation & purification*; D006880:Hydroxy Acids / Q000302:isolation & purification*; D006893:Hydroxyeicosatetraenoic Acids / Q000276:immunology; D008856:Microscopy, Fluorescence; D009090:Mucorales / Q000737:chemistry*
D005227:Fatty Acids; D006880:Hydroxy Acids; D006893:Hydroxyeicosatetraenoic Acids; C069265:3-hydroxy-5,8,11,14-eicosatetraenoic acid