Extracellular FGF-1 inhibits cytoskeletal organization and promotes fibroblast motility.
Previous efforts from this laboratory have established that acidic fibroblast growth factor (FGF-1), either added exogenously or secreted as a biologically active protein, induces a transformed phenotype in primary murine fibroblasts. Experimental studies described here demonstrate that constitutive exposure to extracellular FGF-I results in reduced cell attachment to multiple ligands, inhibition of cytoskeletal organization, and reduced collagen contraction, despite no detectable change in integrin cell surface expression. In addition, FGF-1-transduced fibroblasts demonstrated a > 10-fold increase in migration, an observation correlated with increased tyrosine phosphorylation of p125FAK and p130CAS. Collectively, these results suggest that FGF-1-induced fibroblast transformation includes the involvement of specific FGF receptor-mediated signal transduction cascades targeted to cytoskeletal and focal adhesion structures.
Ding
Q
Q
Department of Surgery, School of Medicine, University of Alabama at Birmingham, 35294, USA.
Gladson
C L
CL
Guidry
C R
CR
Santoro
S A
SA
Dickeson
S K
SK
Shin
J T
JT
Thompson
J A
JA
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects*; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced*; D003599:Cytoskeleton / Q000187:drug effects*; D016220:Fibroblast Growth Factor 1; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D005347:Fibroblasts; D051379:Mice; D008810:Mice, Inbred C57BL
D016220:Fibroblast Growth Factor 1; D005346:Fibroblast Growth Factors