Translocating peptides and proteins and their use for gene delivery.
A dramatic surge in the development of peptides for gene delivery in vitro and in vivo has been witnessed in the past decade. A better understanding of the structural and mechanistic properties of peptides has been an important step for the rational design of optimal peptide-based gene delivery systems. Research has focused on the design of short synthetic peptides that overcome both extracellular and intracellular limitations of other gene delivery systems by binding reversibly and condensing DNA, specifically targeting cells and/or tissues, rapidly releasing plasmids into the cytoplasm and mediating efficient nuclear translocation.
Morris
M C
MC
The Scripps Research Institute, Department of Molecular Biology, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Chaloin
L
L
Heitz
F
F
Divita
G
G
D004247:DNA / Q000378:metabolism; D004337:Drug Carriers; D018390:Gene Targeting / Q000379:methods*; D015316:Genetic Therapy / Q000379:methods; D019913:Nuclear Localization Signals; D010455:Peptides / Q000378:metabolism*; D021381:Protein Transport
D004337:Drug Carriers; D019913:Nuclear Localization Signals; D010455:Peptides; D004247:DNA