Antibacterial activity evaluation of microcin J25 against diarrheagenic Escherichia coli.
The inhibitory activities of known microcins were evaluated against some diarrheagenic Escherichia coli strains. Some antibacterial properties of microcin J25, the most active one, were studied. A rapid two-step purification was performed. The MIC and the minimum bactericidal concentration of J25 against E. coli O157:H7 were 1 and 100 microg ml(-1), respectively. A 10(4)-CFU ml(-1) contamination by this strain was destroyed in milk and meat extract by 6.25 microg of J25 ml(-1) and in half-diluted egg yolk by 50 microg of J25 ml(-1).
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Laboratoire de Génie Protéique et Cellulaire, Pôle Sciences, Université de La Rochelle, La Rochelle, France.
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Gendron-Gaillard
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Cottenceau
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D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001430:Bacteriocins / Q000494:pharmacology*; D003967:Diarrhea / Q000382:microbiology*; D004926:Escherichia coli / Q000145:classification / Q000187:drug effects* / Q000302:isolation & purification; D019453:Escherichia coli O157 / Q000187:drug effects* / Q000302:isolation & purification; D006801:Humans; D008826:Microbial Sensitivity Tests; D010455:Peptides; D011994:Recombinant Proteins / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D010455:Peptides; D011994:Recombinant Proteins; C020411:microcin