Jasmonic acid signaling modulates ozone-induced hypersensitive cell death.
Recent studies suggest that cross-talk between salicylic acid (SA)-, jasmonic acid (JA)-, and ethylene-dependent signaling pathways regulates plant responses to both abiotic and biotic stress factors. Earlier studies demonstrated that ozone (O(3)) exposure activates a hypersensitive response (HR)-like cell death pathway in the Arabidopsis ecotype Cvi-0. We now have confirmed the role of SA and JA signaling in influencing O(3)-induced cell death. Expression of salicylate hydroxylase (NahG) in Cvi-0 reduced O(3)-induced cell death. Methyl jasmonate (Me-JA) pretreatment of Cvi-0 decreased O(3)-induced H(2)O(2) content and SA concentrations and completely abolished O(3)-induced cell death. Cvi-0 synthesized as much JA as did Col-0 in response to O(3) exposure but exhibited much less sensitivity to exogenous Me-JA. Analyses of the responses to O(3) of the JA-signaling mutants jar1 and fad3/7/8 also demonstrated an antagonistic relationship between JA- and SA-signaling pathways in controlling the magnitude of O(3)-induced HR-like cell death.
Rao
M V
MV
Plant Biotechnology Center, Ohio State University, Columbus, Ohio 43210, USA.
Lee
H
H
Creelman
R A
RA
Mullet
J E
JE
Davis
K R
KR
D000085:Acetates / Q000494:pharmacology; D017360:Arabidopsis / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects*; D003517:Cyclopentanes / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000235:genetics; D009154:Mutation; D054883:Oxylipins; D010126:Ozone / Q000633:toxicity*; D010940:Plant Proteins / Q000235:genetics; D030821:Plants, Genetically Modified; D018749:RNA, Plant / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D020156:Salicylic Acid / Q000378:metabolism; D015398:Signal Transduction
D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; D018749:RNA, Plant; D017382:Reactive Oxygen Species; C053376:pathogenesis-related proteins, plant; D010126:Ozone; C011006:jasmonic acid; C072239:methyl jasmonate; D006861:Hydrogen Peroxide; D006899:Mixed Function Oxygenases; C022619:salicylate 1-monooxygenase; D020156:Salicylic Acid