Thyroid oxidase (THOX2) gene expression in the rat thyroid cell line FRTL-5.
A cDNA encoding an NADPH oxidase flavoprotein was isolated from the rat thyroid gland. The predicted 1517-residue polypeptide was 82.5% identical to the human THOX2/DUOX2 and 74% similar to THOX1/DUOX1. Rat THOX2 lacks a stretch of 30 residues, corresponding to one exon in the human gene sequence. THOX2 mRNA was found to be expressed in cultured FRTL-5 cells. The level of THOX2 mRNA was increased by cAMP in these cells and it was decreased in the thyroids of rats treated with the antithyroid drug methimazole, unlike the TPO and NIS mRNAs. Since it was found in the intestine, duodenum, and colon, in addition to thyroid, we suggest that it be called LNOX, the new family of long homologs of NOX flavoproteins rather than THOX and/or DUOX.
Dupuy
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C
Faculté de Pharmacie, INSERM Unité 486, 5 rue J. B. Clément, Ch atenay-Malabry Cedex, 92296, France.
Pomerance
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M
Ohayon
R
R
Noël-Hudson
M S
MS
Dème
D
D
Chaaraoui
M
M
Francon
J
J
Virion
A
A
D000595:Amino Acid Sequence; D000818:Animals; D013956:Antithyroid Agents / Q000494:pharmacology; D015152:Blotting, Northern; D002273:Carcinogens; D002460:Cell Line; D003001:Cloning, Molecular; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D005091:Exons; D005260:Female; D005420:Flavoproteins / Q000096:biosynthesis* / Q000235:genetics*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007453:Iodide Peroxidase / Q000737:chemistry / Q000235:genetics; D008713:Methimazole / Q000494:pharmacology; D008969:Molecular Sequence Data; D009195:Peroxidase / Q000737:chemistry / Q000235:genetics; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013961:Thyroid Gland / Q000201:enzymology*; D013972:Thyrotropin / Q000494:pharmacology; D013997:Time Factors; D014018:Tissue Distribution
D013956:Antithyroid Agents; D002273:Carcinogens; D018076:DNA, Complementary; D005420:Flavoproteins; D012333:RNA, Messenger; D005576:Colforsin; D008713:Methimazole; D013972:Thyrotropin; D006861:Hydrogen Peroxide; D000242:Cyclic AMP; D009195:Peroxidase; D007453:Iodide Peroxidase; D013755:Tetradecanoylphorbol Acetate