title: On the presence and organization of open reading frames of the nonmotile pathogen Brucella abortus similar to class II, III, and IV flagellar genes and to LcrD virulence superfamily.
abstract: Brucellae are pathogenic, nonmotile bacteria that are facultative intracellular parasites. Little is known about the genetics of these bacteria. Open reading frames from Brucella abortus with similarity to the flagellin, M-ring, and hook of related bacteria were discovered. The open reading frames encode proteins of three of the four flagellum gene classes, namely II, III, and IV. A homolog of the LcrD virulence superfamily was also found. This superfamily is involved in type III protein secretion. B. abortus has the potential for motility and type III secretion.
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, National Animal Disease Center, Zoonotic Diseases Research Unit, Ames, Iowa, USA.
authors.forename: S M
authors.initials: SM
authors.lastname: Halling
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D002003:Brucella abortus / Q000235:genetics* / Q000472:pathogenicity; D017931:DNA Primers; D005407:Flagella / Q000235:genetics*; D005408:Flagellin / Q000737:chemistry / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014774:Virulence / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; C075660:Flig protein, Bacteria; D008565:Membrane Proteins; D005408:Flagellin; C071579:LcrD protein, Yersinia