Structure, chromosomal localization, and expression of the Drosophila melanogaster gene encoding sepiapterin reductase.
We have isolated and characterized a Drosophila melanogaster gene encoding the sepiapterin reductase (SR). The gene does not have introns. The 5'- and 3'-RACE analysis, which determined the transcription start point (tsp) and polyadenylation site, respectively, showed that the gene produces single mRNA species. The potential promoter region lacks distinct TATAAA or CCAAT box consensus sequences. RNA blot analysis revealed that the gene encodes a 1.4kb transcript that could be detected throughout development and in both heads and bodies of adults. The Drosophila SR gene maps to 15A on the X chromosome.
Seong
C
C
Department and Institute of Genetic Engineering, Kyung Hee University, 449-701, Yongin City, South Korea.
Baek
K
K
Yoon
J
J
D000429:Alcohol Oxidoreductases / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004331:Drosophila melanogaster / Q000196:embryology / Q000201:enzymology / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D017344:Genes, Insect / Q000235:genetics*; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic; D014960:X Chromosome / Q000235:genetics
D012333:RNA, Messenger; D004247:DNA; D000429:Alcohol Oxidoreductases; C022630:sepiapterin reductase