title: High level, tissue-specific expression of a modified calcitonin/calcitonin gene-related peptide promoter in a human medullary thyroid carcinoma cell line.
abstract: The efficient and high level expression of therapeutic genes in target cells is critical for effective gene therapy. We have developed a novel promoter by utilizing tandem repeats of a tissue-specific regulatory element from the calcitonin/calcitonin gene-related peptide (CT/CGRP) gene placed in close proximity to a basal promoter, thereby removing interstitial sequences. This promoter drives expression of reporter genes at much higher levels than the natural promoter while significantly improving specificity in thyroid C cells.
authors.affiliation: Department of Cancer Genetics, Kolling Institute of Medical Research, Royal North Shore Hospital, St. Leonards, NSW, Australia.
authors.forename: M
authors.initials: M
authors.lastname: Messina
authors.forename: D M
authors.initials: DM
authors.lastname: Yu
authors.forename: D L
authors.initials: DL
authors.lastname: Learoyd
authors.forename: G W
authors.initials: GW
authors.lastname: Both
authors.forename: P L
authors.initials: PL
authors.lastname: Molloy
authors.forename: B G
authors.initials: BG
authors.lastname: Robinson
mesh_terms: D015740:Calcitonin Gene-Related Peptide / Q000096:biosynthesis / Q000235:genetics*; D018276:Carcinoma, Medullary / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D011401:Promoter Regions, Genetic; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D015740:Calcitonin Gene-Related Peptide