title: Novel CNS glutamate receptor subunit genes of Drosophila melanogaster.
abstract: We report the identification and characterization of two genes from Drosophila melanogaster that encode novel ionotropic glutamate receptor proteins, named DGluR-IB and DNMDAR-II, and that are located on chromosome 3L, region 67AB, and the X chromosome, position 2B, respectively. The DGluR-IB full-length cDNA was isolated from Drosophila embryonic and head libraries. The encoded protein of 1,095 amino acids displays high sequence identity (73%) to DGluR-IA. The DNMDAR-II gene was identified by sequence-homology searches in databases. The deduced protein shows moderate sequence identity (29-31%) to the mouse NMDAR2A-D receptor subunits. Whole-mount in situ hybridization on embryos revealed DGluR-IB and DNMDAR-II transcripts in the CNS. Immunofluorescence analysis of the adult fly brain indicates that the DGluR-IB protein is expressed in neurons implicated in the regulation of the circadian clock.
authors.affiliation: Abteilung Neurochemie, Max-Planck-Institut für Hirnforschung, Frankfurt am Main, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Völkner
authors.forename: B
authors.initials: B
authors.lastname: Lenz-Böhme
authors.forename: H
authors.initials: H
authors.lastname: Betz
authors.forename: B
authors.initials: B
authors.lastname: Schmitt
mesh_terms: D000818:Animals; D002490:Central Nervous System / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D015151:Immunoblotting; D008969:Molecular Sequence Data; D017470:Receptors, Glutamate / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D017470:Receptors, Glutamate