Fluorescence resonance energy transfer as a structural tool for nucleic acids.
Fluorescence resonance energy transfer is a spectroscopic method that provides distance information on macromolecules in solution in the range 20-80 A. It is particularly suited to the analysis of the global structure of nucleic acids because the long-range distance information provides constraints when modelling these important structures. The application of fluorescence resonance energy transfer to nucleic acid structure has seen a resurgence of interest in the past decade, which continues to increase. An especially exciting development is the recent extension to single-molecule studies.
Lilley
D M
DM
CRC Nucleic Acid Structure Research Group, Department of Biochemistry, The University of Dundee, Dundee DD1 4HN, UK. dmjilley@bad.dundee.ac.uk
Wilson
T J
TJ
D009690:Nucleic Acid Conformation; D009696:Nucleic Acids / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D009696:Nucleic Acids