Transcription factor expression during pancreatic islet regeneration.
Recent studies by a number of laboratories have identified transcription factors that are involved in pancreatic development. Indeed, marked abnormalities in pancreatic development result from deficiencies in these molecules, which include, among others, PDX-1, islet-1 (Isl-1), and Pax-6. These studies have prompted us to evaluate the expression of Isl-1 and Pax-6 in the pancreas of the interferon-gamma (IFNgamma) transgenic mouse, which exhibits new islet growth and expansion of ducts throughout the life of the animal. We have previously demonstrated that PDX-1 is strikingly expressed in the ducts of the IFNgamma transgenic mouse. This latter observation compelled us to examine expression of hepatocyte nuclear factor-3beta (HNF3beta), which mediates PDX-1 gene transcription, in the IFNgamma transgenic pancreas as well. As a result of these studies, we now demonstrate marked expression of these transcription factors in the pancreatic ducts of IFNgamma transgenic mice. These data suggest a role for these transcription factors during pancreatic regeneration in the IFNgamma transgenic mouse.
Kritzik
M R
MR
Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Krahl
T
T
Good
A
A
Krakowski
M
M
St-Onge
L
L
Gruss
P
P
Wright
C
C
Sarvetnick
N
N
D000818:Animals; D005136:Eye Proteins; D005786:Gene Expression Regulation / Q000502:physiology; D018398:Homeodomain Proteins / Q000096:biosynthesis / Q000235:genetics*; D007328:Insulin / Q000235:genetics*; D007371:Interferon-gamma / Q000235:genetics*; D007515:Islets of Langerhans / Q000502:physiology*; D060850:LIM-Homeodomain Proteins; D051379:Mice; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; D011401:Promoter Regions, Genetic; D012038:Regeneration / Q000235:genetics; D012097:Repressor Proteins; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics*
D005136:Eye Proteins; D018398:Homeodomain Proteins; D007328:Insulin; D060850:LIM-Homeodomain Proteins; D009419:Nerve Tissue Proteins; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; C000605732:Pax6 protein, mouse; D012097:Repressor Proteins; D014157:Transcription Factors; C063843:insulin gene enhancer binding protein Isl-1; D007371:Interferon-gamma