Isolation of amino acids from natural samples using sublimation.
Amino acids have appreciable vapor pressures above 150 degrees C and will sublime under partial vacuum at elevated temperatures without any racemization or decomposition. The recoveries of several amino acids including aspartic acid, serine, glycine, alanine, alpha-aminoisobutyric acid, and valine were optimized by varying the temperature and duration of sublimation. Sublimation has been shown to be a rapid and effective technique for the isolation of amino acids from natural samples for enantiomeric analyses and a good substitute for conventional cation-exchange desalting techniques.
Glavin
D P
DP
Scripps Institution of Oceanography, University of California at San Diego, La Jolla 92093-0212, USA.
Bada
J L
JL
D000418:Albumins / Q000737:chemistry; D000596:Amino Acids / Q000737:chemistry / Q000302:isolation & purification*; D000818:Animals; D002254:Carbonates / Q000737:chemistry; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation / Q000379:methods; D005580:Fossils; D019015:Geologic Sediments / Q000737:chemistry; D008974:Mollusca / Q000737:chemistry; D011312:Pressure; D013237:Stereoisomerism; D013696:Temperature
D000418:Albumins; D000596:Amino Acids; D002254:Carbonates