The relationship between high-molecular-weight glutenin subunit composition and the quality of Japanese hexaploid wheat lines.
To reveal the high-molecular-weight (1-1MW) glutenin subunit composition, the seed storage proteins of 40 Japanese wheat (Triticum aestivum) lines were fractionated by sodium dodecyl sulfate- polyacrylamide gel electrophoresis to determine their HMW glutenin subunit composition. These were identified by comparison of subunit mobility with that previously found in hexaploid wheat. Twelve different, major glutenin HMW subunits were identified. Each line contained three to five subunits, and 11 different glutenin subunit patterns were observed for 11 alleles in Japanese lines. The Glu-1 quality scores were not particularly high for most of the Japanese wheats in the southern part of Japan (Kyushu district). However, the Glu-1 quality scores of several wheat lines in the Hokkaido area (north Japan) were high. South Japanese wheat lines showed specialty allelic variation in the glutenin HMW 145 kfla subunit, different from those in non-Japanese hexaploid wheats.
Nakamura
H
H
Tophoku National Agricultural Experiment Station, Morioka, Iwate, Japan.
D000483:Alleles; D004591:Electrophoresis, Polyacrylamide Gel; D005983:Glutens / Q000031:analogs & derivatives* / Q000737:chemistry / Q000235:genetics; D007564:Japan; D008970:Molecular Weight; D014908:Triticum / Q000737:chemistry / Q000235:genetics / Q000592:standards*
D005983:Glutens; C037302:glutenin