Assessment of two devices for in vitro preparation of human sperm.
The aim of the study was to assess and compare the efficiency of the ZSC-II versus the Sperm Select techniques in preparing human sperm from normozoospermic patients for use in intrauterine insemination and other assisted reproductive technologies. Twenty-five patients were included in the study. Semen was collected at intercourse and processed via 2 sperm preparation methods. Recovery of motile sperm and other sperm qualitative measurements were evaluated before and after ZSC-II and Sperm Select sperm preparation. Sperm qualitative measurements were not significantly different after the ZSC-II and Sperm Select procedures (p > .05). Differences (p < .05) were noted between the 2 procedures in the number of sperm recovered. A higher survival rate (longevity test, 72 h) was observed after ZSC-II sperm preparation and recovery. The ZSC-II procedure yielded higher total motile sperm than the Sperm Select. The superiority in longevity may suggest possible advantages in obtaining higher fertilization and pregnancy rates.
Zavos
P M
PM
Greek-American Andrology Institute of Athens.
Zarmakoupis-Zavos
P N
PN
Correa
J R
JR
Aslanis
P
P
Zarmakoupis
C N
CN
D005307:Fertilization in Vitro; D006801:Humans; D007315:Insemination, Artificial; D008297:Male; D012099:Reproductive Techniques; D013048:Specimen Handling / Q000295:instrumentation / Q000379:methods*; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology