title: Neurotrophin-4/5 promotes proliferation of oligodendrocyte-type-2 astrocytes (O-2A).
abstract: In this study, the ability of neurotrophin-5 (NT-4/5) to promote the proliferation of oligodendrocyte precursor (O-2A) cells has been examined. This has been accomplished by the addition of exogenous NT-4/5 to purified cultures of O-2A cells maintained in an undifferentiated state by the addition of the mitogens platelet derived growth factor (PDGF-AA) and basic fibroblast growth factor (bFGF). Counts of cells incorporating bromodeoxyuridine (BrdU) showed that the addition of NT-4/5 for 24 h increased O-2A cell proliferation by 1.8-fold above that seen in PDGF-AA and bFGF alone. These data demonstrate a previously unidentified role for the neurotrophin NT-4/5 in oligodendrocyte biology.
authors.affiliation: Department of Biochemistry and Molecular Biology, Mayo Medical and Graduate Schools, Rochester, MN 55905, USA.
authors.forename: I A
authors.initials: IA
authors.lastname: Scarisbrick
authors.forename: K
authors.initials: K
authors.lastname: Asakura
authors.forename: M
authors.initials: M
authors.lastname: Rodriguez
mesh_terms: D000818:Animals; D000963:Antimetabolites; D001253:Astrocytes / Q000187:drug effects*; D001973:Bromodeoxyuridine; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D007091:Image Processing, Computer-Assisted; D009046:Motor Neurons / Q000187:drug effects / Q000648:ultrastructure; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009836:Oligodendroglia / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000166:cytology / Q000187:drug effects; D013234:Stem Cells / Q000187:drug effects
chemical_list: D000963:Antimetabolites; D009414:Nerve Growth Factors; C071951:neurotrophin 5; D001973:Bromodeoxyuridine; C073922:neurotrophin 4