A novel, sensitive detection system for high-density microarrays using dendrimer technology.
To improve signal detection on cDNA microarrays, we adapted a fluorescent oligonucleotide dendrimeric signal amplification system to microarray technology. This signal detection method requires 16-fold less RNA for probe synthesis, does not depend on the incorporation of fluorescent dNTPs into a reverse transcription reaction, generates a high signal-to-background ratio, and can be used to allow for multichannel detection on a single chip. Furthermore, since the dendrimers can be detected individually, it may be possible, by employing dendrimer-binding standards, to calculate the numbers of bound cDNAs can be estimated. These features make the dendrimer signal detection reagent ideal for high-throughput functional genomics research.
Stears
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Brigham and Women's Hospital, Department of Medicine, Harvard Institutes of Medicine, Boston, Massachusetts 02115, USA.
Getts
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RC
Gullans
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SR
D001709:Biotechnology / Q000379:methods; D002232:Carbocyanines / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D005456:Fluorescent Dyes / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods; D023281:Genomics / Q000379:methods*; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization / Q000379:methods; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D012015:Reference Standards; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013379:Substrate Specificity; D014158:Transcription, Genetic / Q000235:genetics
D002232:Carbocyanines; D017931:DNA Primers; D018076:DNA, Complementary; D005456:Fluorescent Dyes; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; C089187:cyanine dye 3; C085321:cyanine dye 5