title: The role of structural intersubunit microheterogeneity in the regulation of the activity in hysteresis of ribulose 1, 5-bisphosphate carboxylase/oxygenase.
abstract: Many enzymes are composed of subunits with the identical primary structure. It has been believed that the protein structure of these subunits is the same. Ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO) comprises eight large subunits with the identical amino acid sequence and eight small subunits. Rotation of the side chains of the lysine residues, Lys-21 and Lys-305, in each of the eight large subunits in spinach RuBisCO in two ways produces microheterogeneity among the subunits. These structures are stabilized through hydrogen bonds by water molecules incorporated into the large subunits. This may cause different effects upon catalysis and a hysteretic, time-dependent decrease in activity in spinach RuBisCO. Changing the amino acid residues corresponding to Lys-21 and Lys-305 in non-hysteretic Chromatium vinosum RuBisCO to lysine induces hysteresis and increases the catalytic activity from 8.8 to 15.8 per site per second. This rate is approximately five times higher than that of the higher-plant enzyme.
authors.affiliation: Plant Molecular Physiology, Research Institute of Innovative Technology for the Earth (RITE), Kizu, Kyoto 619-0292, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Uemura
authors.forename: N
authors.initials: N
authors.lastname: Shibata
authors.lastname: Anwaruzzaman
authors.forename: M
authors.initials: M
authors.lastname: Fujiwara
authors.forename: T
authors.initials: T
authors.lastname: Higuchi
authors.forename: H
authors.initials: H
authors.lastname: Kobayashi
authors.forename: Y
authors.initials: Y
authors.lastname: Kai
authors.forename: A
authors.initials: A
authors.lastname: Yokota
mesh_terms: D002384:Catalysis; D002844:Chromatium / Q000201:enzymology; D006860:Hydrogen Bonding; D007700:Kinetics; D008239:Lysine / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D021122:Protein Subunits; D012273:Ribulose-Bisphosphate Carboxylase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D012399:Rotation; D018724:Spinacia oleracea / Q000201:enzymology
chemical_list: D021122:Protein Subunits; D012273:Ribulose-Bisphosphate Carboxylase; D008239:Lysine