High density cultivation of two strains of iron-oxidizing bacteria through reduction of ferric iron by intermittent electrolysis.
Electrolytic cultivation was applied to Leptospirillum ferrooxidans strains P3A and CF27, which use ferrous iron to respire aerobically. Ferrous iron was supplied to the bacteria by intermittent electrolytic reduction of ferric iron as electron shuttle using an electrode. The yield of L. ferrooxidans and strain CF27 reached 20- and 50-fold, respectively, higher density than were achievable yields without electrolysis. The time required to obtain high density depended not on the growth ratio, but rather on the original growth rate of each strain.
Matsumoto
N
N
Department of Bio-Science, Central Research Institute of Electric Power Industry, 1646 Abiko, Abiko-city Chiba 270-1194, Japan. norio@criepi.denken.or.jp
Yoshinaga
H
H
Ohmura
N
N
Ando
A
A
Saiki
H
H
D001709:Biotechnology / Q000379:methods; D002455:Cell Division; D004572:Electrolysis / Q000379:methods*; D006090:Gram-Negative Bacteria / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D010084:Oxidation-Reduction; D013855:Thiobacillus / Q000378:metabolism
D007501:Iron