Centrosome replication in somatic cells: the significance of G1 phase.
Proper cell division requires that the cell be able to form a bipolar spindle during mitosis. To achieve this, the centrosome must be replicated accurately during interphase. Our understanding of the mechanisms that allow centrosome doubling to be coordinated with other cell cycle progression processes is advancing at a rapid pace. Several different experimental systems have been developed that are allowing detailed studies of centrosome replication. For example, the identification of mutants in yeast that are unable to duplicate the SPB accurately during interphase has provided important insights concerning centrosome duplication. In addition, intact embryonic cells and extracts prepared from unfertilized eggs are powerful tools for investigating the molecular regulation of centrosome doubling during the cell cycle. Many of the observations from these embryonic systems are directly applicable to understanding centrosome doubling in somatic cells. Finally, transgenic mouse models and cultured mammalian cell systems have been developed for analyzing the regulation of centrosome doubling in cells with more complex cell cycles. As our knowledge of the cell cycle advances, particularly our understanding of the intricate series of events that must occur for somatic cells to traverse G1 phase, it should be possible to use the systems that have been developed to determine how the replication of the centrosome is coordinated with other cell cycle progression processes. The next few years should see rapid advances in our understanding of this critical cell biological process.
Balczon
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Department of Cell Biology and Neuroscience, University of South Alabama, Mobile 36688, USA.
D000818:Animals; D018385:Centrosome / Q000502:physiology* / Q000648:ultrastructure; D016193:G1 Phase / Q000502:physiology*; D006801:Humans; D051379:Mice; D008941:Spindle Apparatus / Q000502:physiology / Q000648:ultrastructure