Influence of glutathione metabolising enzymes in rats with gram-negative sepsis.
OBJECTIVE
To evaluate the role of glutathione metabolising enzymes in rats with Gram-negative sepsis.


METHODS
University hospital, Sweden.


METHODS
61 male Sprague Dawley rats.


METHODS
Animals were divided into two groups, one of which was given tert-butyl-4-hydroxyanisole (BHA), a powerful inducer of glutathione metabolising enzymes. A gelatine capsule containing bacteria and adjuvant substances was placed in the abdomen. In one group it contained only adjuvant substances, and the controls underwent sham laparotomy.


METHODS
Activities of glutathione metabolising enzymes, and histological effect on pulmonary tissue.


RESULTS
Activities of glutathione metabolising enzymes were reduced in lung tissue after the induction of sepsis. Pre-treatment with BHA increased enzyme activity and reduced the histological changes.


CONCLUSIONS
Glutathione metabolising enzymes may have a role in sepsis, and pre-treatment with BHA seems to prevent histological changes in pulmonary tissue in septic rats.
Hansson
L
L
Department of Surgery, Malmö University Hospital, Sweden.
Seidegård
J
J
Johansson
L
L
Jeppsson
B
B
D000818:Animals; D001442:Bacteroides Infections / Q000201:enzymology*; D001441:Bacteroides fragilis; D004927:Escherichia coli Infections / Q000201:enzymology*; D005978:Glutathione / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008168:Lung / Q000201:enzymology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012128:Respiratory Distress Syndrome / Q000209:etiology
D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D005978:Glutathione