title: Roles of renal dopamine and kallikrein-kinin systems in antihypertensive mechanisms of exercise in rats.
abstract: We have previously shown that both renal dopamine (DA) and kallikrein-kinin systems are activated by exercise in mild hypertensives. We aimed to confirm the effects of exercise on the renal DA system and the stimulatory effects of DA on the renal kallikrein-kinin system in rats. In experiment 1, 12 male Dahl salt-sensitive (DS) rats given a 4% salt diet were divided into two groups. Rats in the exercise group were forced to run at 8 m/min, 60 min/day, 5 days/week for 4 weeks. Daily urinary volume, urinary excretion of sodium, free DA, and kallikrein activity were measured weekly. Renal aromatic-L-amino-acid decarboxylase (AADC) activities were assayed at the end of the experiment. In experiment 2, 15 male Sprague-Dawley (SD) rats were randomly divided into 3 groups, a DA-5 (5 microg of DA/kg/min), a DA-10 (10 microg of DA/kg/min), and a control group. DA or vehicle was administered subcutaneously with an osmotic pump for 2 weeks. Daily urinary volume, urinary excretion of sodium, aldosterone, DA, and kallikrein activity were measured weekly. Plasma renin activity, aldosterone concentration, and renal kallikrein mRNA levels were determined at the end of the experiment. In experiment 1, urinary excretion of free DA and renal AADC activities in the exercise group were significantly higher than those in the non-exercise group at week 4. In experiment 2, renal kallikrein mRNA levels and urinary volume were significantly increased in the DA-10 group compared to the control group, although there were no differences in urinary kallikrein activities. Plasma aldosterone concentration was significantly decreased in the DA-10 group compared to that in the control group despite a lack of differences in plasma renin activities. In conclusion, exercise increased the urinary excretion of free DA, probably through increased renal AADC activity in DS rats. DA amplified renal kallikrein mRNA levels and decreased plasma aldosterone levels, probably through its suppression of aldosterone in the adrenal glands. Activation of the kallikrein-kinin system might be counteracted by post-transcriptional modification of aldosterone. These results suggest that exercise enhances renal dopamine production by activating renal AADC activity, which in turn stimulates the renal kallikrein-kinin system.
authors.affiliation: Department of Internal Medicine, School of Medicine, Fukuoka University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Maeda
authors.forename: M
authors.initials: M
authors.lastname: Sasaguri
authors.forename: T
authors.initials: T
authors.lastname: Sakai
authors.forename: A
authors.initials: A
authors.lastname: Kinoshita
authors.forename: M
authors.initials: M
authors.lastname: Koga
authors.forename: K
authors.initials: K
authors.lastname: Noda
authors.forename: E
authors.initials: E
authors.lastname: Tsuji
authors.forename: M
authors.initials: M
authors.lastname: Ideishi
authors.forename: K
authors.initials: K
authors.lastname: Arakawa
mesh_terms: D000450:Aldosterone / Q000097:blood; D000818:Animals; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000378:metabolism; D001794:Blood Pressure; D015152:Blotting, Northern; D001835:Body Weight; D002316:Cardiotonic Agents / Q000494:pharmacology* / Q000652:urine; D004298:Dopamine / Q000494:pharmacology* / Q000652:urine; D015870:Gene Expression / Q000502:physiology; D006339:Heart Rate; D006973:Hypertension / Q000503:physiopathology*; D016234:Kallikrein-Kinin System / Q000187:drug effects / Q000502:physiology*; D007610:Kallikreins / Q000235:genetics / Q000652:urine; D007668:Kidney / Q000201:enzymology*; D008297:Male; D005082:Physical Exertion / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D020303:Rats, Inbred Dahl; D017207:Rats, Sprague-Dawley; D012083:Renin / Q000097:blood
chemical_list: D002316:Cardiotonic Agents; D012333:RNA, Messenger; D000450:Aldosterone; D007610:Kallikreins; D012083:Renin; D001142:Aromatic-L-Amino-Acid Decarboxylases; D004298:Dopamine