Radish extract stimulates motility of the intestine via the muscarinic receptors.
The effects of radish (Brassica oleraceae, Cruciferae) on gastrointestinal motility were examined using rat intestinal segments with myenteric plexus in-vitro and measuring the intestinal transit of charcoal in-vivo. Radish extract (10 microg mL(-1) to 2 mg mL(-1)) caused a dose-dependent increase in contractions of the duodenum, jejunum and ileum, and 1 mg mL(-1) was the maximum effective dose. The largest contraction by the extract was found in ileal segments. The extract-induced (0.5 mg mL(-1)) ileal contraction was remarkably inhibited by pretreatment of segments with atropine (10(-7) M) for 10 min, but not by hexamethonium (0.5 mM). Moreover, antagonists of the muscarinic receptor reduced the radish-induced ileal contraction by a different ratio. The rank order of inhibitory effects was 4-diphenylacetoxy-N-methyl-(2-chloroethyl)-piperidine methiodide (90.5% of control) > tropicamide (67.4%) > pirenzepine (42.8%) > methoctramine (16.7%). Oral administration of radish extract (300-500 mg kg(-1) body weight) to mice remarkably improved the intestinal transit of charcoal, and this was significantly attenuated by co-administration of atropine (50 mg kg(-1)). Taken together, these results suggest that radish extract stimulates gastrointestinal motility through activation of muscarinic pathways.
Jung
K Y
KY
Department of Pharmacology, School of Medicine, Wonkwang University, Iksan, Chonbuk, Korea. wkuniv@wonkwang.ac.kr
Choo
Y K
YK
Kim
H M
HM
Choi
B K
BK
D000704:Analysis of Variance; D000818:Animals; D001937:Brassica; D004347:Drug Interactions; D005769:Gastrointestinal Motility / Q000187:drug effects*; D007422:Intestines / Q000187:drug effects*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D018727:Muscarinic Antagonists / Q000494:pharmacology*; D009119:Muscle Contraction / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011976:Receptors, Muscarinic / Q000187:drug effects*
D018727:Muscarinic Antagonists; D010936:Plant Extracts; D011976:Receptors, Muscarinic