title: MHC (major histocompatibility complex)-DRB genes and polymorphisms in common marmoset.
abstract: A New World monkey, the common marmoset (Callithrix jacchus), will be used as a preclinical animal model to study the feasibility of cell and gene therapy targeting immunological and hematological disorders. For elucidating the immunogenetic background of common marmoset to further studies, in the present study, polymorphisms of MHC-DRB genes in this species were examined. Twenty-one Caja-DRB exon 2 alleles, including seven new ones, were detected by means of subcloning and the polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) methods followed by nucleotide sequencing. Based on the alignment of these allele sequences, we designed two pairs of specific primers and established a PCR-SSCP method for DNA-based histocompatibility typing of the common marmoset. According to the family segregation data and phylogenetic analyses, we presumed that Caja-DRB alleles could be classified into five different loci. Southern blotting analysis also supported the existence of multiple DRB loci. The patterns of nucleotide substitutions suggests that positive selection operates in the antigen-recognition sites of Caja-DRB genes.
authors.affiliation: Division of Molecular Therapy, Advanced Clinical Research Center (ACRC), The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
authors.forename: M S
authors.initials: MS
authors.lastname: Wu
authors.forename: K
authors.initials: K
authors.lastname: Tani
authors.forename: H
authors.initials: H
authors.lastname: Sugiyama
authors.forename: H
authors.initials: H
authors.lastname: Hibino
authors.forename: K
authors.initials: K
authors.lastname: Izawa
authors.forename: T
authors.initials: T
authors.lastname: Tanabe
authors.forename: Y
authors.initials: Y
authors.lastname: Nakazaki
authors.forename: H
authors.initials: H
authors.lastname: Ishii
authors.forename: J
authors.initials: J
authors.lastname: Ohashi
authors.forename: H
authors.initials: H
authors.lastname: Hohjoh
authors.forename: T
authors.initials: T
authors.lastname: Iseki
authors.forename: A
authors.initials: A
authors.lastname: Tojo
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Y
authors.initials: Y
authors.lastname: Tanioka
authors.forename: K
authors.initials: K
authors.lastname: Tokunaga
authors.forename: S
authors.initials: S
authors.lastname: Asano
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002144:Callithrix / Q000235:genetics* / Q000276:immunology*; D017931:DNA Primers / Q000235:genetics; D019143:Evolution, Molecular; D005091:Exons; D005260:Female; D005802:Genes, MHC Class II; D008297:Male; D008969:Molecular Sequence Data; D010375:Pedigree; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D017386:Sequence Homology, Amino Acid
chemical_list: D017931:DNA Primers