Augmentation of signal transducer and activation of transcription (STAT)6 and STAT3 expression in stimulated B and T cells.
Signal transducer and activation of transcription (STAT)6 has a central role in the signal transduction of interleukin (IL)-4 and IL-13. It has recently been revealed that STAT3 is also involved. STAT6 and STAT3 are expressed ubiquitously; however, it remains unknown how STAT6 and STAT3 expression is regulated. In this study, we found that STAT6 expression was augmented at the transcription level in B and T cells stimulated with anti-IgM antibody and anti-CD40 antibody or PMA and ionomycin, respectively, and that STAT3 expression was similarly augmented in the stimulated B cells. The stimulated B and T cells showed enhancement of STAT6 activation and CD23 expression induced by IL-4 and IL-13. Augmentation of STAT6 and STAT3 would be a mechanism of the amplification of the IL-4 and IL-13 signals in stimulated B and T cells.
Arinobu
Y
Y
Department of Clinical Chemistry and Laboratory Medicine, Kyushu University, Fukuoka, 812-8582, Japan.
Sugimoto
R
R
Akaiwa
M
M
Arima
K
K
Otsuka
T
T
Hamasaki
N
N
Izuhara
K
K
D001402:B-Lymphocytes / Q000378:metabolism*; D015153:Blotting, Western; D019013:CD40 Antigens / Q000378:metabolism; D002273:Carcinogens; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D005786:Gene Expression Regulation; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology / Q000378:metabolism; D018793:Interleukin-13 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D015759:Ionomycin / Q000494:pharmacology; D007476:Ionophores / Q000494:pharmacology; D011233:Precipitin Tests; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D017455:Receptors, IgE / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050796:STAT3 Transcription Factor; D050800:STAT6 Transcription Factor; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013997:Time Factors; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D015854:Up-Regulation
D019013:CD40 Antigens; D002273:Carcinogens; D004268:DNA-Binding Proteins; D007075:Immunoglobulin M; D018793:Interleukin-13; D007476:Ionophores; D012333:RNA, Messenger; D017455:Receptors, IgE; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D050800:STAT6 Transcription Factor; C494101:STAT6 protein, human; D015534:Trans-Activators; D015847:Interleukin-4; D015759:Ionomycin; D013755:Tetradecanoylphorbol Acetate