Field performance of transgenic elite commercial hybrid rice expressing bacillus thuringiensis delta-endotoxin.
Here we describe development of transgenic elite rice lines expressing a Bt fusion gene derived from cryIA(b) and cryIA(c) under the control of rice actinI promoter. The lines used in the study were indica CMS restorer line of Minghui 63 and its derived hybrid rice Shanyou 63. The level of Bt fusion protein CryIA(b)/CryIA(c) detected in Minghui 63 (T51-1) plants was 20 ng/mg soluble protein. The Bt Shanyou 63 was field-tested in natural and repeated heavy manual infestation of two lepidopteran insects, leaffolder and yellow stem borer. The transgenic hybrid plants showed high protection against both insect pests without reduced yield.
Tu
J
J
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China.
Zhang
G
G
Datta
K
K
Xu
C
C
He
Y
Y
Zhang
Q
Q
Khush
G S
GS
Datta
S K
SK
D000199:Actins / Q000235:genetics; D000818:Animals; D001413:Bacillus thuringiensis; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001427:Bacterial Toxins; D002681:China; D004731:Endotoxins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D005818:Genetic Engineering; D006460:Hemolysin Proteins; D006720:Homozygote; D006824:Hybridization, Genetic; D007306:Insecticides / Q000378:metabolism*; D007915:Lepidoptera / Q000502:physiology; D012275:Oryza / Q000235:genetics* / Q000254:growth & development* / Q000469:parasitology; D010572:Pest Control, Biological; D010641:Phenotype; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D019076:Transgenes / Q000235:genetics
D000199:Actins; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; D011993:Recombinant Fusion Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis