Molecular cloning of ring finger protein 21 (RNF21)/interferon-responsive finger protein (ifp1), which possesses two RING-B box-coiled coil domains in tandem.
We have cloned the full length of a novel cDNA, named ring finger protein 21 (RNF21), composed of the RING finger-B box-coiled coil (RBCC) domain and the B30.2 domain, which are characteristic of the RBCC-B30.2 family. As a structural feature, the RNF21 cDNA possessed at least three kinds of isoforms, due to alternative splicing, consisting of the long form with the RBCC-RBCC-B30.2 domain, the medium form with the RBCC-B30.2 domain, and the short form with only the RBCC domain. Moreover, respective transcripts corresponding to the three isoforms were detected in various human organs by reverse transcription-PCR and Northern blot analyses. Interestingly, the medium form of the RNF21 mRNA expressed most predominantly was dramatically up-regulated within 8-16 h by interferon stimulation of HeLa cells. These findings suggest that RNF21 is a downstream gene that may mediate interferon's biological action.
Orimo
A
A
Department of Biochemistry, Saitama Medical School, Saitama, 350-0495, Japan.
Tominaga
N
N
Yoshimura
K
K
Yamauchi
Y
Y
Nomura
M
M
Sato
M
M
Nogi
Y
Y
Suzuki
M
M
Suzuki
H
H
Ikeda
K
K
Inoue
S
S
Muramatsu
M
M
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D019556:COS Cells; D002352:Carrier Proteins / Q000235:genetics*; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007372:Interferons / Q000494:pharmacology; D008297:Male; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000235:genetics; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D020449:Repetitive Sequences, Amino Acid; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D016335:Zinc Fingers / Q000235:genetics*
D002352:Carrier Proteins; D018076:DNA, Complementary; D020033:Protein Isoforms; D012333:RNA, Messenger; C417918:TRIM34 protein, human; D007372:Interferons