New neuropeptides containing carboxy-terminal RFamide and their receptor in mammals.
Only a few RFamide peptides have been identified in mammals, although they have been abundantly found in invertebrates. Here we report the identification of a human gene that encodes at least three RFamide-related peptides, hRFRP-1-3. Cells transfected with a seven-transmembrane-domain receptor, OT7T022, specifically respond to synthetic hRFRP-1 and hRFRP-3 but not to hRFRP-2. RFRP and OT7T022 mRNAs are expressed in particular regions of the rat hypothalamus, and intracerebroventricular administration of hRFRP-1 increases prolactin secretion in rats. Our results indicate that a variety of RFamide-related peptides may exist and function in mammals.
Hinuma
S
S
Pharmaceutical Discovery Research Division, Takeda Chemical Industries Ltd., 10 Wadai, Tsukuba, Ibaraki 300-4293, Japan.
Shintani
Y
Y
Fukusumi
S
S
Iijima
N
N
Matsumoto
Y
Y
Hosoya
M
M
Fujii
R
R
Watanabe
T
T
Kikuchi
K
K
Terao
Y
Y
Yano
T
T
Yamamoto
T
T
Kawamata
Y
Y
Habata
Y
Y
Asada
M
M
Kitada
C
C
Kurokawa
T
T
Onda
H
H
Nishimura
O
O
Tanaka
M
M
Ibata
Y
Y
Fujino
M
M
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D003001:Cloning, Molecular; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D051381:Rats; D018013:Receptors, Neuropeptide / Q000235:genetics / Q000302:isolation & purification*; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D009479:Neuropeptides; C416987:RFamide peptide; D018013:Receptors, Neuropeptide; C058047:arginylphenylalaninamide