Basic fibroblast growth factor may improve devascularized sternal healing.
BACKGROUND
We have shown that a gelatin sheet incorporating basic fibroblast growth factor enhanced bone regeneration of the devascularized sternum. The purpose of this study was to determine if topical use of the gelatin sheet accelerated normal sternal regeneration and bone remodeling.


METHODS
Thirty Wistar rats had median sternotomy and were divided into 3 groups: 10 had the bilateral internal thoracic arteries removed and basic fibroblast growth factor sheet applied on the sternum (group A), 10 had just the bilateral internal thoracic arteries removed (group B), and 10 had intact bilateral internal thoracic arteries (group C).


RESULTS
Four weeks later the peristernal blood flow significantly increased and marked angiogenesis was seen around the sternum in group A. Histologically, the sternum was almost completely healed only in group A. In group A the bone mineral content was highest, but the bone mineral density was similar to that in other groups. The osteoclast index in group A was highest at the border zone of bone formation and remained high in regenerated bone.


CONCLUSIONS
The basic fibroblast growth factor sheet offset sternal ischemia and accelerated normal sternal bone regeneration and remodeling, not only by callus formation but also by callus resorption.
Iwakura
A
A
Department of Cardiovascular Surgery, Kyoto University Graduate School of Medicine and Institute for Frontier Medical Sciences and College of Medical Technology, Kyoto University, Japan.
Tabata
Y
Y
Nishimura
K
K
Nakamura
T
T
Shimizu
Y
Y
Fujita
M
M
Komeda
M
M
D000818:Animals; D015519:Bone Density; D001861:Bone Regeneration / Q000187:drug effects*; D016723:Bone Remodeling / Q000187:drug effects; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage / Q000494:pharmacology*; D005780:Gelatin; D008297:Male; D018919:Neovascularization, Physiologic / Q000187:drug effects; D010010:Osteoclasts / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013249:Sternum / Q000098:blood supply* / Q000166:cytology / Q000187:drug effects; D014945:Wound Healing / Q000187:drug effects*
D016222:Fibroblast Growth Factor 2; D005780:Gelatin