Cloning, characterization, and expression analysis of mouse enamelysin.
Enamelysin is a recently isolated member of the matrix metalloproteinase (MMP) family of extracellular matrix (ECM)-degrading enzymes. Here we describe the isolation and characterization of the mouse enamelysin cDNA. Expression of mouse enamelysin was detectable only in ameloblasts and odontoblasts of developing teeth. Characterization of mouse enamelysin demonstrated that it is highly conserved in both its sequence content and pattern of expression relative to the porcine, human, and bovine homologues previously described.
Caterina
J
J
Matrix Metalloproteinase Unit, National Institute of Dental and Craniofacial Research, Bethesda, MD 20892-4380, USA. jc239h@nih.gov
Shi
J
J
Sun
X
X
Qian
Q
Q
Yamada
S
S
Liu
Y
Y
Krakora
S
S
Bartlett
J D
JD
Yamada
Y
Y
Engler
J A
JA
Birkedal-Hansen
H
H
Simmer
J P
JP
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern / Q000379:methods; D002417:Cattle; D003001:Cloning, Molecular / Q000379:methods*; D018076:DNA, Complementary / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D053519:Matrix Metalloproteinase 20; D020782:Matrix Metalloproteinases / Q000235:genetics*; D051379:Mice; D008963:Molar / Q000201:enzymology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013552:Swine
D018076:DNA, Complementary; C507111:MMP20 protein, human; D053519:Matrix Metalloproteinase 20; D020782:Matrix Metalloproteinases; C507113:Mmp20 protein, mouse