Regulation of the expression of the sea urchin mitochondrial D-loop binding protein during early development.
The Paracentrotus lividus mitochondrial D-loop binding protein (mtDBP) is a DNA-binding protein which is involved in the regulation of sea urchin mtDNA transcription. Immunoblots of Heparin Sepharose-bound proteins at selected early developmental stages, as well as electrophoretic mobility shift assay, show that mtDBP is present in the egg at a concentration of about 1 x 10(6) molecules/egg. Its level increases after fertilization of about twofold, remaining substantially unchanged between 16-h blastula stage and early pluteus stage and declines thereafter. The content of mtDBP mRNA, determined by RNase protection experiments, increases about sevenfold at the 16-h blastula stage compared to the egg. A considerable decrease occurs at the 40-h pluteus stage, which precedes that of the protein. These results suggest that the expression of mtDBP is regulated at transcriptional level up to blastula stage, while other factors, in addition to the level of the RNA, may control the content of this protein in the following stages of embryogenesis.
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Dipartimento di Biochimica e Biologia Molecolare, Università di Bari and Centro Studi sui Mitocondri e Metabolismo Energetico, CNR, Via Orabona 4, Bari, 70126, Italy.
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Polosa
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Milella
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Sagliano
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Gadaleta
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D000818:Animals; D001755:Blastocyst; D015153:Blotting, Western; D004247:DNA / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D006493:Heparin / Q000378:metabolism; D015151:Immunoblotting; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism; D012617:Sea Urchins; D012685:Sepharose / Q000378:metabolism; D013997:Time Factors; D014158:Transcription, Genetic
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; C119291:mitochondrial D-loop-binding protein, Paracentrotus lividus; D006493:Heparin; D004247:DNA; D012685:Sepharose; D012260:Ribonucleases