title: Does seminal fluid viscosity influence sperm chromatin integrity?
abstract: A retrospective study was undertaken to investigate whether viscosity alters sperm chromatin integrity. Semen samples were obtained from 269 men attending the infertility clinic. The viscosity was measured quantitatively by needle and syringe method and the viscosity ratio was calculated against distilled water. The chromatin integrity was evaluated by in vitro decondensation test using 1% SDS and 6 mM EDTA. According to the viscosity ratios the samples were divided into 2 groups: I, normal (ratio < 9, n = 239): and II, abnormal (ratio > 9, n = 30) viscosity. Chromatin integrity was significantly lower in the group with higher viscosity. Significant decrease in sperm count and motility were seen in group II as compared to group I. Thus, hyperviscosity of seminal fluid alters the sperm chromatin integrity.
authors.affiliation: Institute for Research in Reproduction, Parel, Mumbai, India.
authors.forename: K
authors.initials: K
authors.lastname: Gopalkrishnan
authors.forename: V
authors.initials: V
authors.lastname: Padwal
authors.forename: D
authors.initials: D
authors.lastname: Balaiah
mesh_terms: D002843:Chromatin / Q000648:ultrastructure*; D006801:Humans; D008297:Male; D012189:Retrospective Studies; D012661:Semen / Q000502:physiology*; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000648:ultrastructure*; D014783:Viscosity
chemical_list: D002843:Chromatin