A bioluminescent whole-cell reporter for detection of 2, 4-dichlorophenoxyacetic acid and 2,4-dichlorophenol in soil.
A bioreporter was made containing a tfdRP(DII)-luxCDABE fusion in a modified mini-Tn5 construct. When it was introduced into the chromosome of Ralstonia eutropha JMP134, the resulting strain, JMP134-32, produced a sensitive bioluminescent response to 2, 4-dichlorophenoxyacetic acid (2,4-D) at concentrations of 2.0 microM to 5.0 mM. This response was linear (R(2) = 0.9825) in the range of 2.0 microM to 1.1 x 10(2) microM. Saturation occurred at higher concentrations, with maximal bioluminescence occurring in the presence of approximately 1.2 mM 2,4-D. A sensitive response was also recorded in the presence of 2,4-dichlorophenol at concentrations below 1.1 x 10(2) microM; however, only a limited bioluminescent response was recorded in the presence of 3-chlorobenzoic acid at concentrations below 1.0 mM. A significant bioluminescent response was also recorded when strain JMP134-32 was incubated with soils containing aged 2,4-D residues.
Hay
A G
AG
Center for Environmental Biotechnology, University of Tennessee, Knoxville, Knoxville, Tennessee 37996-1605, USA.
Rice
J F
JF
Applegate
B M
BM
Bright
N G
NG
Sayler
G S
GS
D015084:2,4-Dichlorophenoxyacetic Acid / Q000032:analysis*; D000871:Anthelmintics / Q000032:analysis; D002733:Chlorophenols / Q000032:analysis*; D020118:Cupriavidus necator / Q000235:genetics; D017931:DNA Primers; D004926:Escherichia coli / Q000235:genetics; D017930:Genes, Reporter; D006540:Herbicides / Q000032:analysis; D049449:Luminescence; D016254:Mutagenesis, Insertional; D011401:Promoter Regions, Genetic; D012680:Sensitivity and Specificity; D012989:Soil Pollutants / Q000032:analysis*
D000871:Anthelmintics; D002733:Chlorophenols; D017931:DNA Primers; D006540:Herbicides; D012989:Soil Pollutants; D015084:2,4-Dichlorophenoxyacetic Acid; C004762:2,4-dichlorophenol