Inhibition of 1,2-dimethylhydrazine-induced oxidative DNA damage in rat colon mucosa by black tea complex polyphenols.
The effect of black tea polyphenols on 1,2-dimethylhydrazine (DMH)-induced oxidative DNA damage in rat colon mucosa has been investigated. Fischer 344 rats were treated orally with thearubigin (TR) or theafulvin (TFu) for 10 days (40 mg/kg), injected ip with DMH (20 mg/kg) or saline and sacrificed 24 hr after DMH administration. The levels of 8-hydroxy-2'-deoxyguanosine (8-OHdG) were measured in colonic mucosa DNA and expressed as a ratio relative to 2'-deoxyguanosine (2dG). Control rat mucosa had 8-OHdG values of 1.12 +/- 0.14/10(5) dG (mean +/- SEM, n=11), whereas DMH-treated rats significantly higher values (1.52 +/- 0.14/10(5) dG, n=26, P<0.05). Pretreatment of rats with TR had significantly inhibited DMH-induced oxidative DNA damage 0.99 +/- 0.09/10(5) dG, n=10, P<0.05) and a similar, although less marked, effect was observed with TFu (1.15 +/- 0.19/10(5), n=9, P=0.06). These findings confirm that DMH causes oxidative DNA damage in the colon mucosa of rats and demonstrate that this effect is prevented by the consumption of complex polyphenols from black tea.
Lodovici
M
M
Department of Pharmacology, University of Florence, Italy. maura@pharm.unifi.it
Casalini
C
C
De Filippo
C
C
Copeland
E
E
Xu
X
X
Clifford
M
M
Dolara
P
P
D019813:1,2-Dimethylhydrazine / Q000633:toxicity; D000080242:8-Hydroxy-2'-Deoxyguanosine; D000477:Alkylating Agents / Q000633:toxicity; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002273:Carcinogens / Q000633:toxicity; D002392:Catechin / Q000031:analogs & derivatives / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D003110:Colonic Neoplasms / Q000139:chemically induced* / Q000517:prevention & control; D004249:DNA Damage / Q000187:drug effects*; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000032:analysis; D005419:Flavonoids; D007413:Intestinal Mucosa / Q000187:drug effects*; D008297:Male; D010636:Phenols / Q000494:pharmacology*; D008517:Phytotherapy; D011108:Polymers / Q000494:pharmacology*; D059808:Polyphenols; D051381:Rats; D011916:Rats, Inbred F344; D013662:Tea / Q000737:chemistry* / Q000627:therapeutic use
D000477:Alkylating Agents; D000975:Antioxidants; D002273:Carcinogens; D005419:Flavonoids; D010636:Phenols; D011108:Polymers; D059808:Polyphenols; D013662:Tea; C086701:thearubigin; D000080242:8-Hydroxy-2'-Deoxyguanosine; D002392:Catechin; D003849:Deoxyguanosine; D019813:1,2-Dimethylhydrazine