Long-term survival but impaired homeostatic proliferation of Naïve T cells in the absence of p56lck.
Interactions between the T cell receptor (TCR) and major histocompatibility complex antigens are essential for the survival and homeostasis of peripheral T lymphocytes. However, little is known about the TCR signaling events that result from these interactions. The peripheral T cell pool of p56lck (lck)-deficient mice was reconstituted by the expression of an inducible lck transgene. Continued survival of peripheral naïve T cells was observed for long periods after switching off the transgene. Adoptive transfer of T cells from these mice into T lymphopoienic hosts confirmed that T cell survival was independent of lck but revealed its essential role in TCR-driven homeostatic proliferation of naïve T cells in response to the T cell-deficient host environment. These data suggest that survival and homeostatic expansion depend on different signals.
Seddon
B
B
Division of Molecular Immunology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK.
Legname
G
G
Tomlinson
P
P
Zamoyska
R
R
D000818:Animals; D017252:CD3 Complex / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000502:physiology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000502:physiology; D002455:Cell Division; D002470:Cell Survival; D004318:Doxycycline / Q000494:pharmacology; D015870:Gene Expression; D006706:Homeostasis; D008213:Lymphocyte Activation; D018655:Lymphocyte Count; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck) / Q000235:genetics / Q000502:physiology*; D017710:Lymphocyte Transfusion; D008221:Lymphoid Tissue / Q000166:cytology / Q000276:immunology; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D010766:Phosphorylation; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D015398:Signal Transduction; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000502:physiology; D013601:T-Lymphocytes / Q000276:immunology / Q000502:physiology* / Q000637:transplantation; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology; D019076:Transgenes
D017252:CD3 Complex; C121704:CD3 antigen, zeta chain; D019253:Proto-Oncogene Proteins c-bcl-2; D011948:Receptors, Antigen, T-Cell; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); D004318:Doxycycline