Bidirectional translocation of neurofilaments along microtubules mediated in part by dynein/dynactin.
Neuronal cytoskeletal elements such as neurofilaments, F-actin, and microtubules are actively translocated by an as yet unidentified mechanism. This report describes a novel interaction between neurofilaments and microtubule motor proteins that mediates the translocation of neurofilaments along microtubules in vitro. Native neurofilaments purified from spinal cord are transported along microtubules at rates of 100-1000 nm/s to both plus and minus ends. This motion requires ATP and is partially inhibited by vanadate, consistent with the activity of neurofilament-bound molecular motors. Motility is in part mediated by the dynein/dynactin motor complex and several kinesin-like proteins. This reconstituted motile system suggests how slow net movement of cytoskeletal polymers may be achieved by alternating activities of fast microtubule motors.
Shah
J V
JV
Harvard-Massachusetts Institute of Technology Division of Health Sciences and Technology, Cambridge, Massachusetts 02139, USA.
Flanagan
L A
LA
Janmey
P A
PA
Leterrier
J F
JF
D000818:Animals; D002417:Cattle; D000072159:Dynactin Complex; D004398:Dyneins / Q000302:isolation & purification / Q000502:physiology*; D007382:Intermediate Filaments / Q000502:physiology* / Q000648:ultrastructure; D008869:Microtubule-Associated Proteins / Q000302:isolation & purification / Q000502:physiology*; D008870:Microtubules / Q000502:physiology* / Q000648:ultrastructure; D009068:Movement; D009474:Neurons / Q000502:physiology* / Q000648:ultrastructure; D051381:Rats; D013116:Spinal Cord / Q000166:cytology
D000072159:Dynactin Complex; D008869:Microtubule-Associated Proteins; D004398:Dyneins