Multitoxin biosensor-mass spectrometry analysis: a new approach for rapid, real-time, sensitive analysis of staphylococcal toxins in food.
Biomolecular interaction analysis mass spectrometry (BIA-MS) was applied to detection of bacterial toxins in food samples. This two-step approach utilizes surface plasmon resonance (SPR) to detect the binding of the toxin(s) to antibodies immobilized on a surface of a sensor chip. SPR detection is then followed by identification of the bound toxin(s) by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Staphylococcal enterotoxin B (SEB) was readily detected in milk and mushroom samples at levels of 1 ng/ml. In addition, non-specific binding of food components to the immobilized antibody and to the sensor chip surface was detected. To evaluate the applicability of BIA-MS in the analysis of materials containing multiple toxic components, sample containing both SEB and toxic-shock syndrome toxin-1 was analyzed. Both toxins were successfully and simultaneously detected through the utilization of multiaffinity sensor chip surfaces.
Nedelkov
D
D
lntrinsic Bioprobes Inc, Tempe, AZ 85281, USA. ibi@inficad.com
Rasooly
A
A
Nelson
R W
RW
D004768:Enterotoxins / Q000032:analysis* / Q000096:biosynthesis; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013211:Staphylococcus aureus / Q000472:pathogenicity*; D020349:Surface Plasmon Resonance / Q000379:methods*
D004768:Enterotoxins