Anti-CD40, anti-CD3, and IL-2 stimulation induce contrasting changes in CB1 mRNA expression in mouse splenocytes.
The expression and function of cannabinoid receptor 1 (CB1) in mouse immune cells is unclear. Here we show that splenic B cells express more CB1 mRNA than T cells. Furthermore, splenocytes stimulated with the T cell mitogens, PMA/Io and anti-CD3, showed a decrease in CB1 message while cultures stimulated with the B cell mitogen, anti-CD40 antibody, showed an increase in message. In addition, co-treatment with mitogens and IL-2 uniformly caused an increase in CB1 mRNA. It is suggested that signaling pathways activated by T cell mitogens lead to decreased CB1 gene activation while pathways activated by B cell mitogens and IL-2 lead to increased CB1.
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Department of Medical Microbiology and Immunology, University of South Florida, College of Medicine, Tampa, FL 33612, USA.
Newton
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C
Widen
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Friedman
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H
Klein
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D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D001402:B-Lymphocytes / Q000737:chemistry / Q000187:drug effects / Q000276:immunology; D017252:CD3 Complex / Q000276:immunology*; D019013:CD40 Antigens / Q000276:immunology*; D002273:Carcinogens / Q000494:pharmacology; D005260:Female; D015870:Gene Expression / Q000187:drug effects / Q000276:immunology; D007376:Interleukin-2 / Q000276:immunology / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000032:analysis; D043882:Receptors, Cannabinoid; D011955:Receptors, Drug / Q000235:genetics* / Q000276:immunology; D015398:Signal Transduction / Q000187:drug effects / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000906:Antibodies; D017252:CD3 Complex; D019013:CD40 Antigens; D002273:Carcinogens; D007376:Interleukin-2; D012333:RNA, Messenger; D043882:Receptors, Cannabinoid; D011955:Receptors, Drug; D013755:Tetradecanoylphorbol Acetate