Reference values of plasma lipoprotein (a) in newly classified apolipoprotein(a) phenotype groups in the Japanese population.
We tried to establish the reference values of plasma lipoprotein (a) [Lp(a)] concentration in phenotype groups of apoliprotein(a) [apo A] classified by a new criterion. Lp(a) concentration was determined by latex agglutination immunoassay, and apo A was analysed by electrophoresis in sodium dodecyl sulphate-polyacrylamide gel and a Western blotting technique. According to the relative mobility to the apo B-100 band, apo A was classified into 11 isoforms, i.e. F, B, and S1-S9, and the phenotype was defined by their apparent combination. The frequency ratio of single-band versus double-band was approximately 2:1. In 382 cases of single-band, the most frequent phenotype was S5 (24.3%), followed by S4 (17.3%), S6 (15.4%) and S3 (14.4%). In 181 cases of double-band, S5/S6 phenotype was observed most frequently (12.2%). followed by S4/S5 (10.5%) and S3/S6 (7.2%). The reference value was determined between antilogs of the mean +/- 1.96 standard deviation by logarithmic transformation of all observed values for individual phenotype cases. These results suggest that the reference values shown to be variable with apo A phenotypes should be useful for evaluating Lp(a) values in diagnosis of atherosclerosis.
Orisaka
M
M
Iwate Health Service Association, Morioka, Japan. mi-orisaka@mvf.biglobe.ne.jp
Koyamada
K
K
Saika
Y
Y
Sakurabayashi
I
I
D001161:Arteriosclerosis / Q000175:diagnosis; D001797:Blood Protein Electrophoresis; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007564:Japan; D007841:Latex Fixation Tests; D017270:Lipoprotein(a) / Q000097:blood* / Q000145:classification; D010641:Phenotype; D020033:Protein Isoforms / Q000097:blood / Q000145:classification; D012016:Reference Values
D017270:Lipoprotein(a); D020033:Protein Isoforms