Prolactin activates interferon regulatory factor-1 expression in normal lympho-hemopoietic cells.
It has been proposed that prolactin (PRL) is a lympho-hemopoietic growth and differentiation factor. We show here by Western blotting that PRL-receptors (PRL-R) are expressed in normal rat bone marrow and spleen cells. We also show that PRL stimulates the phosphorylation of the PRL-R-associated Janus tyrosine kinase (JAK)-2 in rat bone marrow and spleen cells. This leads to the activation and subsequent binding of signal transducer and activator of transcription (Stat) 5b to an interferon regulatory factor-1 (IRF-1) gamma activation sequence (GAS) as visualized by electromobility shift assay. As shown after reverse transcription of mRNA by polymerase chain reaction, PRL, at physiological concentrations (0.01 microg/ml), stimulates the expression of the IRF-1 gene in these normal cells. PRL could thus affect several aspects of the immune response.
Dogusan
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Z
Department of Pharmacology, Medical School, Free University of Brussels (VUB), Laarbeeklaan, 103, B-1090 Brussels, Belgium.
Book
M L
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Verdood
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Yu-Lee
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Hooghe-Peters
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D000818:Animals; D001854:Bone Marrow Cells / Q000276:immunology; D002460:Cell Line; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D050856:Interferon Regulatory Factor-1; D007371:Interferon-gamma / Q000502:physiology; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D008894:Milk Proteins; D010750:Phosphoproteins / Q000235:genetics*; D011388:Prolactin / Q000494:pharmacology / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011981:Receptors, Prolactin / Q000235:genetics*; D050799:STAT5 Transcription Factor; D013154:Spleen / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology; D015534:Trans-Activators / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics
D004268:DNA-Binding Proteins; D050856:Interferon Regulatory Factor-1; C494259:Irf1 protein, rat; D008894:Milk Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger; D011981:Receptors, Prolactin; D050799:STAT5 Transcription Factor; D015534:Trans-Activators; D014157:Transcription Factors; D007371:Interferon-gamma; D011388:Prolactin