title: [Analysis of natural medicines by micellar electrokinetic chromatography (MEKC)--determination of calycosin in Astragali radix].
abstract: We previously reported a HPLC identification method for Astragali Radix and its fluid extract using calycosin as a marker substance. However, it took about 40 min for one run using gradient elution. Therefore, we alternatively employed capillary electrophoresis (CE) for the analysis of calycosin in those materials. As a result, calycosin was clearly separated from the other components in Astragali Radix and its fluid extract by micellar electrokinetic chromatography (MEKC) mode with sodium dodecyl sulfate (SDS) within 5 min. Then we determined the content of calycosin in a drinkable preparation. Validation for the developed method was also performed in accordance with the ICH guideline.
authors.affiliation: Analytical Chemistry Department, Tanabe Seiyaku Co., Ltd., Osaka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kuwahara
authors.forename: T
authors.initials: T
authors.lastname: Nakamura
authors.forename: A
authors.initials: A
authors.lastname: Hashimoto
authors.forename: H
authors.initials: H
authors.lastname: Nishi
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D007529:Isoflavones / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D007529:Isoflavones; D010936:Plant Extracts; C121707:7,3'-dihydroxy-4'-methoxyisoflavone