Escherichia coli is able to produce heterologous tetraheme cytochrome c(3) when the ccm genes are co-expressed.
The production of Desulfovibrio vulgaris Hildenborough cytochrome c(3) (M(r) 13000), which is a tetraheme cytochrome, in Escherichia coli was examined. This cytochrome was successfully produced in an E. coli strain co-expressing the ccmABCDEFGH genes involved in the cytochrome c maturation process. The apocytochrome c(3) was matured in either anaerobic or aerobic conditions, but aerobic growth in the presence of delta-aminolevulinic acid was found to be best for cytochrome c(3) production. Site-directed mutagenesis was performed to investigate the effect of the presence of four amino acids in between the two cysteines of the heme binding sites 2 and 4 on the maturation of holocytochrome c(3) in E. coli.
Herbaud
M L
ML
Unite de Bioénergétique et Ingénierie des Protéines, IBSM-CNRS, Marseilles, France.
Aubert
C
C
Durand
M C
MC
Guerlesquin
F
F
Thöny-Meyer
L
L
Dolla
A
A
D000622:Aminolevulinic Acid / Q000494:pharmacology; D001665:Binding Sites; D003574:Cytochrome c Group / Q000096:biosynthesis / Q000235:genetics*; D016969:Desulfovibrio vulgaris / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006418:Heme / Q000737:chemistry; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D019897:Periplasm / Q000201:enzymology; D010957:Plasmids
D003574:Cytochrome c Group; D006418:Heme; D000622:Aminolevulinic Acid; C017165:cytochrome c(3)