New virulence-activated and virulence-repressed genes identified by systematic gene inactivation and generation of transcriptional fusions in Bordetella pertussis.
An in silico scan of the partially completed genome sequence of Bordetella pertussis and analyses of transcriptional fusions generated with a new integrational vector were used to identify new potential virulence genes. The genes encoding a putative siderophore receptor, adhesins, and an autotransporter protein appeared to be regulated in a manner similar to Bordetella virulence genes by the global virulence regulator BvgAS. In contrast, the gene encoding a putative intimin-like protein appeared to be repressed under conditions of virulence.
Antoine
R
R
INSERM U447, Institut de Biologie de Lille, Institut Pasteur de Lille, 59019 Lille Cedex, France.
Alonso
S
S
Raze
D
D
Coutte
L
L
Lesjean
S
S
Willery
E
E
Locht
C
C
Jacob-Dubuisson
F
F
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D001886:Bordetella pertussis / Q000235:genetics* / Q000472:pathogenicity*; D004352:Drug Resistance, Microbial / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D020868:Gene Silencing; D005798:Genes, Bacterial; D005839:Gentamicins; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D013489:Suppression, Genetic; D014158:Transcription, Genetic; D014774:Virulence / Q000235:genetics*
D001426:Bacterial Proteins; D005839:Gentamicins; D011993:Recombinant Fusion Proteins