title: Rapid and simple determination of the Escherichia coli phylogenetic group.
abstract: Phylogenetic analysis has shown that Escherichia coli is composed of four main phylogenetic groups (A, B1, B2, and D) and that virulent extra-intestinal strains mainly belong to groups B2 and D. Actually, phylogenetic groups can be determined by multilocus enzyme electrophoresis or ribotyping, both of which are complex, time-consuming techniques. We describe a simple and rapid phylogenetic grouping technique based on triplex PCR. The method, which uses a combination of two genes (chuA and yjaA) and an anonymous DNA fragment, was tested with 230 strains and showed excellent correlation with reference methods.
authors.affiliation: Laboratoire d'études de génétique bactérienne dans les infections de l'enfant (EA3105), Université Denis Diderot-Paris 7, Hôpital Robert Debré, Paris, France.
authors.forename: O
authors.initials: O
authors.lastname: Clermont
authors.forename: S
authors.initials: S
authors.lastname: Bonacorsi
authors.forename: E
authors.initials: E
authors.lastname: Bingen
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004798:Enzymes / Q000302:isolation & purification; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics* / Q000472:pathogenicity; D006801:Humans; D007231:Infant, Newborn; D016920:Meningitis, Bacterial / Q000382:microbiology; D010802:Phylogeny; D021521:Ribotyping / Q000379:methods; D014774:Virulence
chemical_list: D004798:Enzymes