Observations on the crystallization of oligogalacturonates.
Oligogalacturonates were produced by the limited enzymic hydrolysis of polygalacturonic acid and purified by ion-exchange chromatography. The fractions obtained were of limited polydispersity, determined by analytical ion-exchange chromatography. Oligomers with an average degree of polymerization of 10-15 were readily crystallized from aqueous salt solutions at neutral pH as single crystals. Crystal morphology of the salts examined, Na+, K+ and Ca2+ were characteristic of the salt. The wide-angle X-ray diffraction patterns obtained for the sodium salt were consistent with published fibre diffraction data of this salt form.
Rigby
N M
NM
Food Quality and Materials Science, Institute of Food Research, Norwich Laboratory, Colney, UK. neil.rigby@bbsrc.ac.uk
MacDougall
A J
AJ
Ring
S G
SG
Cairns
P
P
Morris
V J
VJ
Gunning
P A
PA
D002852:Chromatography, Ion Exchange; D003461:Crystallography; D006603:Hexuronic Acids / Q000737:chemistry*; D006868:Hydrolysis; D008854:Microscopy, Electron; D009844:Oligosaccharides / Q000737:chemistry; D010368:Pectins / Q000737:chemistry; D012492:Salts / Q000494:pharmacology; D014961:X-Ray Diffraction
D006603:Hexuronic Acids; D009844:Oligosaccharides; D012492:Salts; C007819:galacturonic acid; D010368:Pectins