The presence and specificity of crustacean cardioactive peptide (CCAP)-immunoreactivity in gastropod neurons.
CCAP-like immunoreactivity was detected in central neurons with small and medium diameters in both Helix and Lymnaea CNS. The intensity of immunoreactivity showed seasonal changes with a maximum intensity during spring. The overwhelming majority of nerve cell bodies exhibiting CCAP immunoreactivity is located in the cerebral and parietal ganglia of both Helix and Lymnaea. The neurons of pleural and buccal ganglia were devoid of CCAP-immunoreactivity. Following preabsorbtion of CCAP antibody in 1:15000 dilution with 10(-3) M CCAP or CCAP-related peptide (Helix -CCAP), immunoreactivity could not be observed in neurons, demonstrating the specificity of the antibody to CCAP-related molecules in both Helix and Lymnaea.
Hernádi
L
L
Department of Experimental Zoology, Balaton Limnological Research Institute of the Hungarian Academy of Sciences, Tihany. hernadi@tres.blki.hu
Agricola
H J
HJ
D000595:Amino Acid Sequence; D000818:Animals; D002490:Central Nervous System / Q000378:metabolism; D006372:Helix, Snails / Q000378:metabolism*; D007150:Immunohistochemistry; D008195:Lymnaea / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D009479:Neuropeptides / Q000737:chemistry / Q000378:metabolism*
D009479:Neuropeptides; C058620:crustacean cardioactive peptide