Rat homologue of the human M(r) 110000 antigen is the protein that expresses widely in various tissues.
The rat homologue of the human M(r) 110000 antigen, which cross-reacts with anti-carcinoembryonic antigen antibodies, was isolated from a rat lung cDNA library. The deduced amino acid sequence revealed a signal peptide, cysteine-rich and immunoglobulin-like region, serine-threonine region, and N-glycosylation sites in the extracellular portion. Northern blot analysis demonstrated a wide distribution of the mRNA in adult rat tissues and A10 rat vascular smooth muscle cells. Therefore, the rat homologue of the human M(r) 110000 antigen may be a receptor or a cell adhesion molecule rather than a specific carcinogenic antigen.
Nakane
T
T
Department of Pharmacology, Shinshu University School of Medicine, Asahi 3-1-1, 390-8621, Matsumoto, Japan. nakane@sch.md.shinshu-u.ac.jp
Inada
Y
Y
Itoh
F
F
Chiba
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002272:Carcinoembryonic Antigen / Q000235:genetics* / Q000302:isolation & purification; D002460:Cell Line; D002478:Cells, Cultured; D015723:Gene Library; D006801:Humans; D008168:Lung / Q000378:metabolism*; D008969:Molecular Sequence Data; D009131:Muscle, Smooth, Vascular / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D017386:Sequence Homology, Amino Acid
D002272:Carcinoembryonic Antigen; D012333:RNA, Messenger