title: Evaluation of the abbott LCx HIV-1 RNA quantitative, a new assay for quantitative determination of human immunodeficiency virus type 1 RNA.
abstract: A new quantitative reverse transcription (RT)-PCR assay for human immunodeficiency virus type 1 (HIV-1) RNA (Abbott LCx HIV RNA Quantitative assay) has been compared with the Organon NucliSens assay on 521 retrospective samples obtained from HIV-1-positive patients monitored during highly active antiretroviral therapy, 79 of whom were assayed also by the Chiron Quantiplex 3.0 system and on characterized panels. The LCx system showed a moderate correlation (r = 0.795) and gave higher results than the NucliSens system on 245 of 327 concordant positive samples, with similar sensitivity. Correlation with Quantiplex system results was higher (r = 0.943). LCx reproducibility was very good; the procedure was simple, well controlled, and rapid (up to 48 results in 7 h). The HIV RNA quantitative assay on the LCx system is suitable for routine use.
authors.affiliation: Microbiology, "L. Sacco" Teaching Hospital, Milan, Italy.
authors.forename: N
authors.initials: N
authors.lastname: Zanchetta
authors.forename: G
authors.initials: G
authors.lastname: Nardi
authors.forename: L
authors.initials: L
authors.lastname: Tocalli
authors.forename: L
authors.initials: L
authors.lastname: Drago
authors.forename: C
authors.initials: C
authors.lastname: Bossi
authors.forename: F R
authors.initials: FR
authors.lastname: Pulvirenti
authors.forename: C
authors.initials: C
authors.lastname: Galli
authors.forename: M R
authors.initials: MR
authors.lastname: Gismondo
mesh_terms: D023241:Antiretroviral Therapy, Highly Active; D015658:HIV Infections / Q000097:blood / Q000188:drug therapy*; D006679:HIV Seropositivity / Q000097:blood / Q000188:drug therapy*; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D012367:RNA, Viral / Q000097:blood*; D011933:Reagent Kits, Diagnostic; D012044:Regression Analysis; D015203:Reproducibility of Results; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D019562:Viral Load
chemical_list: D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic