[Comparison of 2 techniques for the laboratory diagnosis of influenza virus infections].
The clinical or epidemiological influenza diagnosis require fast, sensitive and accessible techniques for small laboratories. In order to investigate the sensitivity of the methods currently used in Argentina, the rapid diagnosis by indirect immunofluorescent assay (IF) was compared to the rapid viral culture in MDCK cells. The diagnosis of influenza virus infection was performed on 81 nasal and pharyngeal swabs collected from outpatients with upper respiratory infection, influenza-like syndrome. The samples were collected during 1998 winter season and both techniques were tested. The IF specificity and sensitivity obtained were 91.9% and 59.5%, respectively. In the selection of the assay to be used for influenza diagnosis, the limitations of the simplest techniques such as IF should be considered. Furthermore, it is advisable to set up an optimized culture method in complex laboratories since culture is the only technique which allows the reference centers to perform the full characterization of the isolates.
Savy
V L
VL
Departamento Virología, Instituto Nacional de Enfermedades Infecciosas-ANLIS, Buenos Aires, Argentina. vrmalbra@datamarkets.com.ar
Baumeister
E G
EG
Campos
A M
AM
Pontoriero
A
A
D000328:Adult; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D001118:Argentina / Q000453:epidemiology; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D004285:Dogs; D019084:Fluorescent Antibody Technique, Indirect; D006168:Guinea Pigs; D006386:Hemagglutination Tests; D006801:Humans; D009980:Influenza A virus / Q000276:immunology / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D008875:Middle Aged; D009296:Nasal Cavity / Q000821:virology*; D009787:Occupational Medicine; D010614:Pharynx / Q000821:virology*; D012621:Seasons; D012680:Sensitivity and Specificity; D013997:Time Factors; D014776:Virus Cultivation
D000914:Antibodies, Viral