Absence of cholinergic sympathetic innervation from limb muscle vasculature in rats and mice.
Although the existence of cholinergic sympathetic vasodilatory innervation in limb muscle vasculature is well established for some species, previous pharmacological studies have failed to reveal the presence of such innervation in rats. Recently, Schafer and colleagues [Schafer, M.K., Eiden, L.E., Weihe, E., 1998. Cholinergic neurons and terminal fields revealed by immunohistochemistry for the vesicular acetylcholine transporter. II. The peripheral nervous system. Neuroscience 84(2), 361-376] reported that vesicular acetylcholine transporter immunoreactivity (VAChT-IR), a marker for cholinergic terminals, is present in the innervation of the microvasculature of rat hindlimb skeletal muscle and concluded that rats possess cholinergic sympathetic innervation of limb muscle vasculature. Because of our interest in identifying targets of cholinergic sympathetic neurons, we have analyzed the transmitter properties of the innervation of muscle vessels in rat and mouse limbs. We found that the innervation of vasculature in muscle is noradrenergic, exhibiting robust catecholamine histofluorescence and immunoreactivity for tyrosine hydroxylase (TH) and the peptide transmitters, neuropeptide Y (NPY) and occasionally vasoactive intestinal peptide (VIP). In contrast, cholinergic phenotypic markers,VAChT-IR and acetylcholinesterase (AChE) activity, are absent. Neuron cell bodies in sympathetic ganglia, retrogradely labeled with injections of tracer into limb muscles, also lacked VAChT but contained TH-IR. The innervation of large extramuscular feed arteries in hindlimbs was also devoid of cholinergic markers, as were the cell bodies of sympathetic neurons innervating extramuscular femoral arteries. These results, like those of previous physiological studies, provide no evidence for the presence of cholinergic sympathetic innervation of muscle vasculature in rats or mice.
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Neural Development Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892-4062, USA. guidryg@ninds.nih.gov
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D000110:Acetylcholinesterase / Q000032:analysis; D000320:Adrenergic Fibers / Q000737:chemistry / Q000201:enzymology*; D000818:Animals; D001158:Arteries / Q000294:innervation*; D002196:Capillaries / Q000294:innervation*; D002352:Carrier Proteins / Q000032:analysis; D002799:Cholinergic Fibers / Q000737:chemistry / Q000201:enzymology*; D005260:Female; D005728:Ganglia, Sympathetic / Q000166:cytology; D006614:Hindlimb; D008297:Male; D026901:Membrane Transport Proteins; D051379:Mice; D008813:Mice, Inbred ICR; D018482:Muscle, Skeletal / Q000098:blood supply*; D009474:Neurons / Q000737:chemistry / Q000201:enzymology / Q000648:ultrastructure; D009478:Neuropeptide Y / Q000032:analysis; D009638:Norepinephrine / Q000502:physiology; D051381:Rats; D011914:Rats, Inbred BN; D020318:Rats, Long-Evans; D017207:Rats, Sprague-Dawley; D013045:Species Specificity; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis; D014660:Vasoactive Intestinal Peptide / Q000032:analysis; D014664:Vasodilation / Q000502:physiology; D050494:Vesicular Acetylcholine Transport Proteins; D033921:Vesicular Transport Proteins
D002352:Carrier Proteins; D026901:Membrane Transport Proteins; D009478:Neuropeptide Y; C493460:Slc18a3 protein, mouse; C493461:Slc18a3 protein, rat; D050494:Vesicular Acetylcholine Transport Proteins; D033921:Vesicular Transport Proteins; D014660:Vasoactive Intestinal Peptide; D014446:Tyrosine 3-Monooxygenase; D000110:Acetylcholinesterase; D009638:Norepinephrine