Electron crystal structure of the transcription factor and DNA repair complex, core TFIIH.
Core TFIIH from yeast, made up of five subunits required both for RNA polymerase II transcription and nucleotide excision DNA repair, formed 2D crystals on charged lipid layers. Diffraction from electron micrographs of the crystals in negative stain extended to about 13 angstrom resolution, and 3D reconstruction revealed several discrete densities whose volumes corresponded well with those of individual TFIIH subunits. The structure is based on a ring of three subunits, Tfb1, Tfb2, and Tfb3, to which are appended several functional moieties: Rad3, bridged to Tfb1 by SsI1; SsI2, known to interact with Tfb2; and Kin28, known to interact with Tfb3.
Chang
W H
WH
Department of Structural Biology, Stanford University School of Medicine, California 94305, USA.
Kornberg
R D
RD
D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D003460:Crystallization; D018844:Cyclin-Dependent Kinases; D004265:DNA Helicases / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D004260:DNA Repair; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D007091:Image Processing, Computer-Assisted; D050356:Lipid Metabolism; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D008958:Models, Molecular; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D020836:Protein Structure, Quaternary; D011994:Recombinant Proteins / Q000737:chemistry / Q000648:ultrastructure; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins; D035381:TATA-Binding Protein Associated Factors; D035362:Transcription Factor TFIID; D051758:Transcription Factor TFIIH; D014157:Transcription Factors / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure*; D019473:Transcription Factors, TFII
D005656:Fungal Proteins; D046911:Macromolecular Substances; D011994:Recombinant Proteins; C083908:SSL1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C490039:TAF6 protein, S cerevisiae; D035381:TATA-Binding Protein Associated Factors; C086993:TFB1 protein, S cerevisiae; D035362:Transcription Factor TFIID; D014157:Transcription Factors; D019473:Transcription Factors, TFII; D051758:Transcription Factor TFIIH; D017346:Protein Serine-Threonine Kinases; D018844:Cyclin-Dependent Kinases; C084210:Kin28 protein kinase, S cerevisiae; D000251:Adenosine Triphosphatases; C061897:Rad3 protein, S cerevisiae; D004265:DNA Helicases