title: Genetics of alpha-amanitin resistance in a natural population of Drosophila melanogaster.
abstract: The genetic basis of variation in resistance to natural toxins is of interest for both ecological and evolutionary genetics. The wide variety of larval resources used by Drosophila, both within and between species, makes flies an excellent system for studying causes and consequences of selection resulting from exposure to natural toxins associated with different resources. In this study we carry out a genetic analysis of alpha-amanitin resistance in a population sample of Drosophila melanogaster. Data from mapping crosses of chromosome III support a role for a naturally occurring polymorphism in a multidrug resistance gene (Mdr65A) in alpha-amanitin resistance. However, there are no amino acid differences between resistant and sensitive chromosomes at Mdr65A. Therefore, if Mdr65A mutants contribute to the difference between alpha-amanitin-resistant and alpha-amanitin-sensitive third chromosome lines, the underlying cause is a gene regulatory mutation.
authors.affiliation: Section of Integrative Biology and Institute for Cellular & Molecular Biology, University of Texas, Patterson Labs, C0930, Austin, TX 78712, USA. djbegun@mail.utexas.edu
authors.forename: D J
authors.initials: DJ
authors.lastname: Begun
authors.forename: P
authors.initials: P
authors.lastname: Whitley
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B; D000546:Amanitins / Q000494:pharmacology*; D000818:Animals; D002874:Chromosome Mapping; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics*; D004351:Drug Resistance / Q000235:genetics; D018432:Drug Resistance, Multiple / Q000235:genetics; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D005819:Genetic Markers; D019476:Insect Proteins / Q000235:genetics*; D011110:Polymorphism, Genetic
chemical_list: D018435:ATP Binding Cassette Transporter, Subfamily B; D000546:Amanitins; D029721:Drosophila Proteins; D004791:Enzyme Inhibitors; D005819:Genetic Markers; D019476:Insect Proteins; C069936:mdr65 protein, Drosophila