[Peripheral tissue microdialysis technique in unrestrained, conscious animals].
The microdialysis procedure had been developed in the past 2 to 3 decades to determine levels of drug and endogenous compounds in several organs under physiological conditions. Advantages of microdialysis include: minimal stress on the experimental animals; studies may be done in unrestrained, conscious animals, and multiple determination can be made without concern for excess blood loss from small animals; and measurements can be made of drug and/or metabolites at multiple sites in the animal. By employing the microdialysis technique, I developed the method of multiple sampling for a long term period from an animal under the freely moving condition. In addition, I developed a novel microdialysis probe that was applied to several peripheral tissues and/or organs. In this paper, I will describe the fundamental procedure for peripheral tissues and/or organs such as the jugular vein and liver, using subcutaneous and ocular microdialysis sampling in unrestrained, conscious animals.
Kurata
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Department of Pharmacology, School of Medicine, Showa University, Tokyo, Japan.
D000818:Animals; D005123:Eye / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D017551:Microdialysis / Q000379:methods*; D010599:Pharmacokinetics; D051381:Rats