title: K+ and ionic strength directly influence the autophosphorylation activity of the putative turgor sensor KdpD of Escherichia coli.
abstract: The membrane-bound histidine kinase KdpD is a putative turgor sensor that regulates, together with the response regulator KdpE, the expression of the kdpFABC operon coding for the high affinity K(+)-uptake system KdpFABC of Escherichia coli. To elucidate the nature of the primary stimulus for KdpD, we developed an in vitro assay based on right-side-out membrane vesicles. Conditions were varied inside and outside of the vesicles, and KdpD autophosphorylation activity was tested. It was shown that an increase of the ionic strength inside the vesicles was accompanied by an increase of the autophosphorylation activity of KdpD with ATP. However, K(+) at concentrations higher than 1 mm inhibited KdpD autophosphorylation activity. This K(+)-specific effect was not observed with KdpD-Arg-511 --> Gln, a KdpD derivative, which causes K(+)-independent kdpFABC expression. When the osmolality outside the vesicles was increased, autophosphorylation activity of KdpD was stimulated, whereby salts were more effective than sugars. Treatment of the vesicles with amphipathic compounds did not affect KdpD autophosphorylation activity. Based on these results it is proposed that changes of intracellular parameters elicited by K(+) limitation or osmotic upshock directly influence KdpD autophosphorylation activity, whereby K(+) has an inhibitory and ionic strength a stimulatory effect.
authors.affiliation: Universität Osnabrück, Fachbereich Biologie/Chemie, Abteilung Mikrobiologie, D-49069 Osnabrück, Germany. jung_k@biologie.uni-osnabrueck.de
authors.forename: K
authors.initials: K
authors.lastname: Jung
authors.forename: M
authors.initials: M
authors.lastname: Veen
authors.forename: K
authors.initials: K
authors.lastname: Altendorf
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins; D009994:Osmolar Concentration; D010766:Phosphorylation; D011188:Potassium / Q000494:pharmacology*; D011494:Protein Kinases / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D012965:Sodium Chloride; D000255:Adenosine Triphosphate; C489580:KdpD protein, E coli; D011494:Protein Kinases; C075616:kdpD protein, Bacteria; D011188:Potassium