title: Use of isoptin for counteracting the effect of methol on cardiac activity in experimental animals.
abstract: The purpose of the present study is to use isoptin in order to lower intracellular Ca2+ concentration for counteracting the effect of methol on cardiac activity in acute intoxication of the experimental animals. Adult male Wistar rats were used - 8 animals/lot anaesthesized with urethan. ECG activity was recorded every 15 minutes, for 360 minutes. Administration (oral) of methol in a dose of 310 mg/kg/bw. (DL100) produced sinusal bradycardia, which became more marked later on. Isoptin administration in a dose of 5 mg/kg/bw. in rats with 310 mg/kg/bw. methol decreased the heart rate, the lowest value occurring 120 minutes after administration, followed by a slow increase until the end of the recording. When isoptin was given in a dose of 2.5 mg/kg/bw. to rats with 310 mg/kg/bw. methol, the decrease of the heart rate was much slower than before, for a period of 255 minutes, followed by a slight increase until the end of the recording. Isoptin counteracts the bradycardia - producing effect of methol in rats during the study time (6 hours), and the effect after this period of time requires further investigations.
authors.affiliation: Medical and Pharmaceutical University Tg-Mureş, Romania. CMSSC@netsoft.ro
authors.forename: J
authors.initials: J
authors.lastname: Domahidi
authors.forename: K
authors.initials: K
authors.lastname: Péter
authors.forename: J
authors.initials: J
authors.lastname: Farkas
mesh_terms: D000627:Aminophenols / Q000008:administration & dosage / Q000633:toxicity*; D000818:Animals; D001919:Bradycardia / Q000139:chemically induced / Q000517:prevention & control; D002121:Calcium Channel Blockers / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D006339:Heart Rate / Q000187:drug effects*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014700:Verapamil / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D000627:Aminophenols; D002121:Calcium Channel Blockers; C014112:N-methyl-4-aminophenol; D014700:Verapamil