NO(x) contamination in laboratory ware and effect of countermeasures.
Contamination of various types of laboratory wares with NO(x) (NO(-)(2) and NO(-)(3)) was assessed systematically and the effect of extensive washing as a countermeasure was evaluated. Mean NO(x) contamination arising from a model procedure for NO(x) determination in plasma was 0.93 microM (range, 0.35-1.49 microM). The major source of contamination included conical tubes (54.8%) and pipette tips used for transfer of solution (12.3-16.3%). Except for soft glassware, most NO(x) contamination could be washed out by pure water. Although NO(x) contamination in respective laboratory wares could be reduced below detection levels by extensive washing, summation of the contamination through the model procedure could not be completely abolished (but the effect of washing persisted at least 10 days). Heavy contamination was noted in glassware (especially soft glass) and ultrafiltration units, which was difficult to remove. Several types of vacuum blood sampling tubes contained various levels of NO(x). Our results indicated that a small but significant amount of contamination remained in laboratory ware even after extensive washing, and that it is advisable to avoid the use of glassware (soft glass), ultrafiltration units, and vacuum blood sampling tubes during the processing of clinical sampling for the measurement of NO(x).
Ishibashi
T
T
Department of Pharmacology, Division of Endocrinology, Department of Internal Medicine, Kanazawa Medical University, Daigaku 1-1, Uchinada, Ishikawa, 920-0293, Japan. ishitaka@kanazawa-med.ac.jp
Himeno
M
M
Imaizumi
N
N
Maejima
K
K
Nakano
S
S
Uchida
K
K
Yoshida
J
J
Nishio
M
M
D001800:Blood Specimen Collection / Q000295:instrumentation; D002498:Centrifugation / Q000295:instrumentation; D004864:Equipment and Supplies / Q000592:standards*; D005898:Glass; D009566:Nitrates / Q000032:analysis*; D009573:Nitrites / Q000032:analysis*; D010969:Plastics; D012107:Research Design; D013048:Specimen Handling / Q000295:instrumentation; D014462:Ultrafiltration / Q000295:instrumentation
D009566:Nitrates; D009573:Nitrites; D010969:Plastics