title: Interaction between inflammatory cells and heparin-surface-modified intraocular lens.
abstract: OBJECTIVE
  To investigate the interaction and adherence of inflammatory cells to a heparin-surface-modified intraocular lens (HSM IOL).
  
  
  METHODS
  Department of Ophthalmology, Tokyo Medical University Hospital, Tokyo, Japan.
  
  
  METHODS
  Splenic mononuclear leukocytes from rats with experimental autoimmune uveitis were cultured with the optic of an HSM IOL for 96 hours. The number of adherent cells on the HSM IOL surface was measured with and without the addition of interphotoreceptor retinoid-binding protein and concanavalin A (ConA) to the culture medium. The adherent cells were observed under a light microscope or a scanning electron microscope.
  
  
  RESULTS
  Interphotoreceptor retinoid-binding protein and ConA increased the number of adherent cells on the HSM IOL relative to the control. Adherent cells on the HSM IOL were small and round, considered to be mainly lymphocytes.
  
  
  CONCLUSIONS
  Activated lymphocytes tended to adhere to the surface of the HSM IOL.
authors.affiliation: Department of Ophthalmology, Tokyo Medical University Hospital, Tokyo, Japan. takaoeye@tkh.att.ne.jp
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
authors.forename: N
authors.initials: N
authors.lastname: Yamakawa
authors.forename: T
authors.initials: T
authors.lastname: Mizusawa
authors.forename: M
authors.initials: M
authors.lastname: Usui
mesh_terms: D000818:Animals; D001327:Autoimmune Diseases; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible; D003208:Concanavalin A / Q000187:drug effects / Q000378:metabolism; D004195:Disease Models, Animal; D005136:Eye Proteins; D005343:Fibrinolytic Agents; D006493:Heparin; D007910:Lenses, Intraocular; D019904:Polymethyl Methacrylate; D051381:Rats; D011917:Rats, Inbred Lew; D012177:Retinol-Binding Proteins / Q000187:drug effects / Q000378:metabolism; D013154:Spleen / Q000166:cytology; D013499:Surface Properties; D014602:Uvea / Q000648:ultrastructure; D014606:Uveitis, Anterior / Q000276:immunology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D000099214:Retinol-Binding Proteins, Interstitial
chemical_list: D020099:Coated Materials, Biocompatible; D003208:Concanavalin A; D005136:Eye Proteins; D005343:Fibrinolytic Agents; D006493:Heparin; D019904:Polymethyl Methacrylate; D012177:Retinol-Binding Proteins; D000099214:Retinol-Binding Proteins, Interstitial