title: Selective corticotropin-releasing factor type 1 receptor antagonist blocks conditioned fear-induced release of noradrenaline in the hypothalamic paraventricular nucleus of rats.
abstract: The effects of conditioned fear on the release of noradrenaline in the hypothalamic paraventricular nucleus (PVN) and the involvement of corticotropin-releasing factor (CRF) receptor type 1 (CRFR1) in conditioned fear-induced changes in noradrenaline release were examined by intracerebral microdialysis in rats. Conditioned fear was produced by placing animals into a box where they had previously been exposed to a 5-min period of electric footshock, 135 min prior to the start of experiment. Conditioned fear for 20 min produced a significant increase in the release of noradrenaline in the PVN. Intraperitoneal preadministration of a selective nonpeptidic CRFR1 antagonist, CRA1000, completely blocked the conditioned fear-induced release of noradrenaline. These results suggest that CRFR1 is involved in the release of noradrenaline in the hypothalamic PVN induced by conditioned fear.
authors.affiliation: Departments of Physiology and Medicine, Nippon Medical School, Sendagi, Bunkyo-ku, Tokyo, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Otagiri
authors.forename: I
authors.initials: I
authors.lastname: Wakabayashi
authors.forename: T
authors.initials: T
authors.lastname: Shibasaki
mesh_terms: D000818:Animals; D003213:Conditioning, Psychological / Q000187:drug effects / Q000502:physiology*; D004597:Electroshock; D005239:Fear / Q000187:drug effects / Q000502:physiology*; D006614:Hindlimb; D008297:Male; D017551:Microdialysis; D009638:Norepinephrine / Q000037:antagonists & inhibitors*; D010286:Paraventricular Hypothalamic Nucleus / Q000187:drug effects / Q000378:metabolism*; D011725:Pyridines / Q000494:pharmacology*; D011743:Pyrimidines / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D018019:Receptors, Corticotropin-Releasing Hormone / Q000037:antagonists & inhibitors*; D000099052:CRF Receptor, Type 1
chemical_list: D011725:Pyridines; D011743:Pyrimidines; D018019:Receptors, Corticotropin-Releasing Hormone; C117406:CRA1000; D000099052:CRF Receptor, Type 1; D009638:Norepinephrine