Interaction between inflammatory cells and heparin-surface-modified intraocular lens.
OBJECTIVE
To investigate the interaction and adherence of inflammatory cells to a heparin-surface-modified intraocular lens (HSM IOL).


METHODS
Department of Ophthalmology, Tokyo Medical University Hospital, Tokyo, Japan.


METHODS
Splenic mononuclear leukocytes from rats with experimental autoimmune uveitis were cultured with the optic of an HSM IOL for 96 hours. The number of adherent cells on the HSM IOL surface was measured with and without the addition of interphotoreceptor retinoid-binding protein and concanavalin A (ConA) to the culture medium. The adherent cells were observed under a light microscope or a scanning electron microscope.


RESULTS
Interphotoreceptor retinoid-binding protein and ConA increased the number of adherent cells on the HSM IOL relative to the control. Adherent cells on the HSM IOL were small and round, considered to be mainly lymphocytes.


CONCLUSIONS
Activated lymphocytes tended to adhere to the surface of the HSM IOL.
Tanaka
T
T
Department of Ophthalmology, Tokyo Medical University Hospital, Tokyo, Japan. takaoeye@tkh.att.ne.jp
Yamakawa
N
N
Mizusawa
T
T
Usui
M
M
D000818:Animals; D001327:Autoimmune Diseases; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible; D003208:Concanavalin A / Q000187:drug effects / Q000378:metabolism; D004195:Disease Models, Animal; D005136:Eye Proteins; D005343:Fibrinolytic Agents; D006493:Heparin; D007910:Lenses, Intraocular; D019904:Polymethyl Methacrylate; D051381:Rats; D011917:Rats, Inbred Lew; D012177:Retinol-Binding Proteins / Q000187:drug effects / Q000378:metabolism; D013154:Spleen / Q000166:cytology; D013499:Surface Properties; D014602:Uvea / Q000648:ultrastructure; D014606:Uveitis, Anterior / Q000276:immunology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D000099214:Retinol-Binding Proteins, Interstitial
D020099:Coated Materials, Biocompatible; D003208:Concanavalin A; D005136:Eye Proteins; D005343:Fibrinolytic Agents; D006493:Heparin; D019904:Polymethyl Methacrylate; D012177:Retinol-Binding Proteins; D000099214:Retinol-Binding Proteins, Interstitial