EGF triggers caveolin redistribution from the plasma membrane to the early/sorting endocytic compartment of hepatocytes.
In this study, we demonstrate that, in rat liver, epidermal growth factor (EGF) is responsible for the partial redistribution of caveolin-1 from the plasma membrane into the early/sorting endocytic compartment. Highly purified endosomes and plasma membrane fractions were isolated from control rat liver and from rats injected with EGF or pIgA for different times. Whereas in subcellular fractions from control hepatocytes most of caveolin was concentrated in the plasma membrane and the receptor-recycling fractions, after EGF injection there was a significant redistribution of caveolin toward the early/sorting (CURL) endocytic fractions. The recruitment of caveolin into the endocytic compartment was not induced by pIgA.
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Departament de Biologia Cellular, Institut dí Investigacions Biomèdiques August Pi Sunyer, Facultat de Medicina, Universitat de Barcelona, Casanova 143. 08036-, Barcelona, Spain.
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D000818:Animals; D051242:Caveolin 1; D022461:Caveolins / Q000378:metabolism*; D002451:Cell Compartmentation; D002462:Cell Membrane / Q000378:metabolism*; D004705:Endocytosis; D011992:Endosomes / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000502:physiology*; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism*; D007070:Immunoglobulin A / Q000494:pharmacology; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013347:Subcellular Fractions / Q000378:metabolism; D051040:Vesicle-Associated Membrane Protein 3
C495537:Cav1 protein, rat; D051242:Caveolin 1; D022461:Caveolins; D007070:Immunoglobulin A; D008565:Membrane Proteins; D051040:Vesicle-Associated Membrane Protein 3; C034639:polymeric IgA; D004815:Epidermal Growth Factor