A single CTL clone can recognize a naturally processed HIV-1 epitope presented by two different HLA class I molecules.
Although it is known that a single peptide can be recognized by CTL restricted to two MHC class I alleles, there is no direct evidence for presentation of a single peptide by two MHC class I molecules. Furthermore, it is unclear whether such peptides are presented to the same T cell or to different T cells. Our previous study suggested that CTL recognition of the human immunodeficiency virus-1 (HIV-1) Pol HIV-B35-SF2-24 epitope (IPLTEEAEL) occurs via both HLA-B35 and HLA-B51 restriction. Here we provide the first direct evidence that a single CTL clone can recognize this peptide presented by both HLA-B35 and HLA-B51. Furthermore, we directly purified this peptide eluted from both HLA-B*3501 and HLA-B*5101 molecules isolated from target cells infected with HIV-1 recombinant vaccinia virus. These results demonstrate that HIV-B35-SF2-24 is a naturally processed peptide which is presented by both HLA-B*3501 and HLA-B*5101. TCR analysis of one CTL clone suggested that it is a single clone. B*3501-SF2-24-tetrameric complexes inhibited both HLA-B*3501- and HLA-B*5101-restricted recognition of this clone, suggesting that the TCR of this clone cross-recognize the structure of both HLA class I-peptide complexes.
Tomiyama
H
H
Division of Viral Immunology, Center for AIDS Research, Kumamoto University, Japan.
Yamada
N
N
Komatsu
H
H
Hirayama
K
K
Takiguchi
M
M
D000595:Amino Acid Sequence; D017951:Antigen Presentation; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D018984:Epitopes, T-Lymphocyte; D015497:HIV-1 / Q000276:immunology*; D015235:HLA-B Antigens / Q000502:physiology*; D015797:HLA-B35 Antigen / Q000502:physiology*; D059949:HLA-B51 Antigen; D006801:Humans; D008969:Molecular Sequence Data; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014616:Vaccinia virus / Q000276:immunology
D018984:Epitopes, T-Lymphocyte; D015235:HLA-B Antigens; D015797:HLA-B35 Antigen; D059949:HLA-B51 Antigen