Relationship between mechanical properties and pentosidine in tendon: effects of age, diet restriction, and aminoguanidine in broiler breeder hens.
Nonenzymatic glycosylation contributes to the formation of crosslinks, which leads to the structural and functional deterioration of tissue protein. The accumulation of these crosslinks in tissue proteins has been implicated in the alteration of biomechanical properties of connective tissues. The objective of this study was to determine whether tendon breaking time (TBT) and tendon breaking strength (TBS) of the flexor perforans et perforatus digiti iii tendon were related to concentrations of pentosidine in tendons (Pt) of broiler breeder hens from 8 to 125 wk of age. In addition, effects of diet restriction (DR) and a crosslinking inhibitor, aminoguanidine (AG) on Pt, TBS, and TBT were determined. Female chicks (n = 450) were randomly assigned to four treatment groups immediately after hatch: ad libitum-fed (AL); diet-restricted (DR; 60% of AL); and AL and DR groups supplemented with 1.35 mg/kg BW per day AG in the feed (AL+AG and DR+AG, respectively). In AL hens, Pt increased with increasing age (P < or = 0.0001). Concurrently, an age-related parallel increase was found for TBS (P < or = 0.0001) and TBT (P < or = 0.0001). Rate of Pt accumulation was lower in DR (P < or = 0.001), TBS (P < or = 0.01), and TBT (P < or = 0.02) hens compared with AL hens. Concentration of Pt in the AL + AG group was lower (P < or = 0.0002) than in the AL group; TBS and TBT (P < or = 0.01) followed a similar pattern. Supplementation of DR with AG did not affect Pt, TBS, or TBT. The age-related increase in Pt and loss of elasticity in the tendon was retarded by diet restriction and AG.
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Division of Animal and Veterinary Sciences, College of Agriculture, Forestry, and Consumer Sciences, West Virginia University, Morgantown 26506-6108, USA.
Kenney
P B
PB
Al-Humadi
N H
NH
Klandorf
H
H
D000375:Aging; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives* / Q000032:analysis*; D001696:Biomechanical Phenomena; D002645:Chickens / Q000502:physiology*; D004032:Diet; D004548:Elasticity; D005260:Female; D005508:Food Deprivation; D006146:Guanidines / Q000008:administration & dosage*; D008239:Lysine / Q000031:analogs & derivatives* / Q000032:analysis*; D012044:Regression Analysis; D013710:Tendons / Q000737:chemistry* / Q000502:physiology*; D013718:Tensile Strength
D006146:Guanidines; D001120:Arginine; C062187:pentosidine; D008239:Lysine; C004479:pimagedine