Isolation of a novel ubiquitin-like protein from Pleurotus ostreatus mushroom with anti-human immunodeficiency virus, translation-inhibitory, and ribonuclease activities.
A glycoprotein, with a ubiquitin-like N-terminal sequence, has been prepared from an extract of fruiting bodies of the mushroom Pleurotus ostreatus, using a procedure which included ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on SP-Sepharose and Mono Q and gel filtration on Superdex 75. It exhibited a molecular weight of 12.5 kDa and was unadsorbed on DEAE-cellulose and Mono Q, but adsorbed on Affi-blue gel and SP-Sepharose. It inhibited translation in a rabbit reticulocyte lysate system (IC(50) = 160 nM) and exhibited low ribonucleolytic activity (14 micro/mg) toward yeast tRNA. It also expressed an inhibitory activity toward human immunodeficiency virus-1 reverse transcriptase, which could be enhanced by succinylation.
Wang
H X
HX
Department of Microbiology, China Agricultural University, Beijing, China.
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D000595:Amino Acid Sequence; D000818:Animals; D019380:Anti-HIV Agents / Q000494:pharmacology*; D002852:Chromatography, Ion Exchange; D006023:Glycoproteins / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D015497:HIV-1 / Q000187:drug effects*; D008969:Molecular Sequence Data; D020076:Pleurotus / Q000737:chemistry* / Q000201:enzymology; D014176:Protein Biosynthesis / Q000187:drug effects*; D011817:Rabbits; D012260:Ribonucleases / Q000378:metabolism*; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D014452:Ubiquitins / Q000737:chemistry
D019380:Anti-HIV Agents; D006023:Glycoproteins; D014452:Ubiquitins; D012260:Ribonucleases