[Change in the spectra of peroxidases in regenerants of Stachys sieboldii (Miq.) as a result of hormonal and mutagenic effects].
Nodular segments of the stem and stolons of aseptic Stachys sieboldii (Miq.) plants were cultured in Gamborg's B5 medium at a low (1-2 mg/l) or high (10 mg/l) concentration of cytokinins (6-BAP, kinetin) and produced many adventitious buds and shoots without callus formation. Several plants were regenerated from nodular segments incubated at various concentrations of 5-bromodeoxiuridine (5-BdU) for three weeks. All regenerants were morphologically normal. Peroxidase electrophoretic patterns were analyzed to determine whether somaclonal variation is associated with S. sieboldii micropropagation. The frequency of changed forms was higher with the high 6-BAP concentration and with mutagenic treatment. Changed forms were not detected in plants grown at a low cytokinin concentration, which can be used in S. sieboldii micropropagation.
Khusseĭn
I A
IA
Timiryazev Agricultural Academy, Russian Academy of Sciences, Moscow, Russia.
Kochneva
E Z
EZ
Khadeeva
N V
NV
D001973:Bromodeoxyuridine; D003583:Cytokinins / Q000378:metabolism; D009153:Mutagens / Q000494:pharmacology*; D010544:Peroxidases / Q000235:genetics*; D010937:Plant Growth Regulators / Q000502:physiology*; D010944:Plants / Q000201:enzymology* / Q000378:metabolism
D003583:Cytokinins; D009153:Mutagens; D010937:Plant Growth Regulators; D010544:Peroxidases; D001973:Bromodeoxyuridine