title: A cDNA encoding a chitinase from the epithelial cell line of chironomus tentans (Insecta, diptera) and its functional expression.
abstract: A cDNA coding for chitinase was isolated from Chironomus cells, which possesses conserved regions I and II characteristic for family 18 chitinases, a C-terminus enriched in Glu and Pro without the typical "PEST-region," putative glycosylation sites, a reduced number of C-terminal cysteines, and no typical chitin binding domain. Northern blots revealed one specific signal with an apparent size of 2.3 kb. The cDNA was expressed in the baculovirus/Spodoptera system as a His-tag fusion protein, which was secreted as a functionally active enzyme into the medium and could be separated from endogenous viral and Spodoptera-specific chitinases.
authors.affiliation: Entwicklungs- und Molekularbiologie der Tiere, Heinrich-Heine-Universität Düsseldorf, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Feix
authors.forename: S
authors.initials: S
authors.lastname: Glöggler
authors.forename: M
authors.initials: M
authors.lastname: Londershausen
authors.forename: W
authors.initials: W
authors.lastname: Weidemann
authors.forename: K D
authors.initials: KD
authors.lastname: Spindler
authors.forename: M
authors.initials: M
authors.lastname: Spindler-Barth
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D020134:Catalytic Domain; D002460:Cell Line; D002683:Chironomidae / Q000201:enzymology*; D002688:Chitinases / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary; D004847:Epithelial Cells / Q000166:cytology; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D018411:Spodoptera / Q000166:cytology
chemical_list: D018076:DNA, Complementary; D011993:Recombinant Fusion Proteins; D002688:Chitinases