Detection and quantitation of human immunodeficiency virus type 1 in the female genital tract. The Division of AIDS Treatment Research Initiative 009 Study Group.
Human immunodeficiency virus type 1 (HIV-1) was detected in the genital tracts of 59% of 225 women by RNA PCR and in 7% of the women by culture. In a comparison of two sampling methods, endocervical swabs were more sensitive than cervicovaginal lavage for HIV-1 RNA detection by PCR but not by culture and their sensitivity was independent of the concentration of HIV-1 RNA.
Baron
P
P
St. Luke's Hospital, New York, New York 10025, USA.
Bremer
J
J
Wasserman
S S
SS
Nowicki
M
M
Driscoll
B
B
Polsky
B
B
Kovacs
A
A
Reichelderfer
P S
PS
D003430:Cross-Sectional Studies; D005260:Female; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014626:Vaginal Smears / Q000379:methods*
D012367:RNA, Viral