HPLC determination of guaifenesin with selected medications on underivatized silica with an aqueous-organic mobile phase.
A high performance liquid chromatography procedure has been developed for the simultaneous determination of guaifenesin pseudoephedrine-dextromethorphan and guaifenesin-pseudoephedrine in commercially available capsule dosage forms and guaifenesin-codeine in a commercial cough syrup dosage form. The separation and quantitation are achieved on a 25-cm underivatized silica column using a mobile phase of 60:40%) v/v 6.25 mM phosphate buffer, pH 3.0 - acetonitrile at a flow rate of 1 ml min(-1) with detection of all analytes at 216 nm. The separation is achieved within 10 min for each drug mixture. The method showed linearity for the guaifenesin-pseudoephedrine-dextromethorphan mixture in the 50-200, 7.5-30 and 2.5-10, microg ml(-1) ranges, respectively. The intra- and inter-day RSDs ranged from 0.23 to 4.20%, 0.18 to 2.85%, and 0.13 to 5.04% for guaifenesin, pseudoephedrine, and dextromethorphan, respectively. The guaifenesin pseudoephedrine mixture yielded linear ranges of 25-100 and 3.75-15 microg ml(-1) and intra- and inter-day RSDs ranged from 0.65 to 4.18% and 0.23 to 3.00% for guaifenesin and pseudoephedrine, respectively. The method showed linearity for the guaifenesin-codeine mixture in the 25-100 and 2.5-10 microg ml(-1) ranges and RSDs ranged from 0.37 to 4.25% and 0.14 to 2.08% for guaifenesin and codeine, respectively.
Wilcox
M L
ML
Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, The University of Georgia, Athens 30602-2352, USA.
Stewart
J T
JT
D000996:Antitussive Agents / Q000032:analysis; D001993:Bronchodilator Agents / Q000032:analysis; D002214:Capsules; D002851:Chromatography, High Pressure Liquid; D003061:Codeine / Q000032:analysis; D003915:Dextromethorphan / Q000032:analysis; D004338:Drug Combinations; D004809:Ephedrine / Q000032:analysis; D005100:Expectorants / Q000032:analysis*; D006140:Guaifenesin / Q000032:analysis*; D007202:Indicators and Reagents; D012015:Reference Standards; D015203:Reproducibility of Results; D012822:Silicon Dioxide; D012996:Solutions
D000996:Antitussive Agents; D001993:Bronchodilator Agents; D002214:Capsules; D004338:Drug Combinations; D005100:Expectorants; D007202:Indicators and Reagents; D012996:Solutions; D006140:Guaifenesin; D003915:Dextromethorphan; D012822:Silicon Dioxide; D004809:Ephedrine; D003061:Codeine