Characterisation of a cysteine protease cDNA from Lolium multiflorum leaves and its expression during senescence and cytokinin treatment.
A cysteine protease cDNA clone (See1) highly homologous to barley aleurain was isolated from Lolium multiflorum leaves. During leaf senescence, expression of the See1 mRNA and protein was strongly enhanced. In dark-incubated leaf segments, cytokinin delayed senescence and reduced expression of both See1 mRNA and protein.
Li
Q
Q
Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, Ceredigion SY23 3EB, UK.
Bettany
A J
AJ
Donnison
I
I
Griffiths
C M
CM
Thomas
H
H
Scott
I M
IM
D000595:Amino Acid Sequence; D016922:Cellular Senescence / Q000235:genetics; D003546:Cysteine Endopeptidases / Q000096:biosynthesis / Q000235:genetics*; D003583:Cytokinins / Q000494:pharmacology*; D018076:DNA, Complementary / Q000032:analysis; D018744:DNA, Plant / Q000032:analysis; D015870:Gene Expression / Q000187:drug effects; D008129:Lolium / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D003583:Cytokinins; D018076:DNA, Complementary; D018744:DNA, Plant; D012333:RNA, Messenger; D003546:Cysteine Endopeptidases