Neurotrophin-4/5 promotes proliferation of oligodendrocyte-type-2 astrocytes (O-2A).
In this study, the ability of neurotrophin-5 (NT-4/5) to promote the proliferation of oligodendrocyte precursor (O-2A) cells has been examined. This has been accomplished by the addition of exogenous NT-4/5 to purified cultures of O-2A cells maintained in an undifferentiated state by the addition of the mitogens platelet derived growth factor (PDGF-AA) and basic fibroblast growth factor (bFGF). Counts of cells incorporating bromodeoxyuridine (BrdU) showed that the addition of NT-4/5 for 24 h increased O-2A cell proliferation by 1.8-fold above that seen in PDGF-AA and bFGF alone. These data demonstrate a previously unidentified role for the neurotrophin NT-4/5 in oligodendrocyte biology.
Scarisbrick
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IA
Department of Biochemistry and Molecular Biology, Mayo Medical and Graduate Schools, Rochester, MN 55905, USA.
Asakura
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K
Rodriguez
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D000818:Animals; D000963:Antimetabolites; D001253:Astrocytes / Q000187:drug effects*; D001973:Bromodeoxyuridine; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D007091:Image Processing, Computer-Assisted; D009046:Motor Neurons / Q000187:drug effects / Q000648:ultrastructure; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009836:Oligodendroglia / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000166:cytology / Q000187:drug effects; D013234:Stem Cells / Q000187:drug effects
D000963:Antimetabolites; D009414:Nerve Growth Factors; C071951:neurotrophin 5; D001973:Bromodeoxyuridine; C073922:neurotrophin 4