title: Clindamycin promotes phagocytosis and intracellular killing of periodontopathogenic bacteria by crevicular granulocytes: an in vitro study.
abstract: Phagocytosis of periodontopathogenic bacteria by crevicular polymorphonuclear neutrophil granulocytes (PMNs) plays a key role in the aetiology of periodontitis. Antimicrobials such as clindamycin have been proven to be effective in treating progressive forms of this disease. Therefore, the purpose of this study was to determine the effect of clindamycin on the phagocytosing properties of gingival crevicular PMNs obtained from 16 patients with rapidly progressive periodontitis (RPP), eight with localized juvenile periodontitis (LJP), 12 with adult periodontitis (AP) and 13 periodontally healthy controls. The phagocytosis assay was performed with the two strains Porphyromonas gingivalis ATCC 33277 and Actinobacillus actinomycetemcomitans Tanner FDC 44 on a slide. Phagocytosis and intracellular killing were assessed by fluorescence microscopy after staining with acridine orange. The addition of clindamycin elevated the percentage of phagocytosing PMNs in periodontitis patients and controls regardless of whether P. gingivalis or A. actinomycetemcomitans was used as test strain. In granulocytes of healthy controls an enhancement of the intracellular killing of both strains was observed if clindamycin was added. Besides the antimicrobial effect, the enhancement of the phagocytosis might be an additional indication for treatment of periodontitis patients with clindamycin.
authors.affiliation: Department of Medical Microbiology, University Hospital of Jena, Semmelweisstr. 4, D-07740 Jena, Germany. eick@bach.med.uni-jena.de
authors.forename: S
authors.initials: S
authors.lastname: Eick
authors.forename: W
authors.initials: W
authors.lastname: Pfister
authors.forename: D
authors.initials: D
authors.lastname: Fiedler
authors.forename: E
authors.initials: E
authors.lastname: Straube
mesh_terms: D000328:Adult; D016976:Aggregatibacter actinomycetemcomitans / Q000187:drug effects*; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002981:Clindamycin / Q000494:pharmacology* / Q000627:therapeutic use; D006098:Granulocytes / Q000187:drug effects* / Q000382:microbiology / Q000502:physiology; D006801:Humans; D010518:Periodontitis / Q000188:drug therapy / Q000382:microbiology*; D010587:Phagocytosis / Q000187:drug effects*; D016966:Porphyromonas gingivalis / Q000187:drug effects*; D018709:Statistics, Nonparametric
chemical_list: D000900:Anti-Bacterial Agents; D002981:Clindamycin