title: Inhibition of tumor necrosis factor-alpha-induced apoptosis by Asparagus cochinchinensis in Hep G2 cells.
abstract: A human hepatoma cell line, Hep G2 cells, is a reliable system for the study of alcohol-induced hepatotoxicity. In this study, we investigated the effect of an aqueous extract of Asparagus cochinchinensis(MERRIL) (Liliaceae) roots (ACAE) on ethanol (EtOH)-induced cytotoxicity in Hep G2 cells. ACAE (1-100 microg/ml) dose-dependently inhibited the EtOH-induced tumor necrosis factor-alpha (TNF-alpha) secretion. ACAE (1-100 microg/ml) also inhibited the EtOH and TNF-alpha-induced cytotoxicity. Furthermore, we found that ACAE inhibited the TNF-alpha-induced apoptosis of Hep G2 cells. These results suggest that ACAE may prevent the EtOH-induced cytotoxicity through inhibition of the apoptosis of Hep G2 cells.
authors.affiliation: College of Pharmacy, Wonkwang University School of Medicine, Center of Oriental Medicinal Science, Wonkwang University, Iksan, 570-749, Chonbuk, South Korea.
authors.forename: H N
authors.initials: HN
authors.lastname: Koo
authors.forename: H J
authors.initials: HJ
authors.lastname: Jeong
authors.forename: J Y
authors.initials: JY
authors.lastname: Choi
authors.forename: S D
authors.initials: SD
authors.lastname: Choi
authors.forename: T J
authors.initials: TJ
authors.lastname: Choi
authors.forename: Y S
authors.initials: YS
authors.lastname: Cheon
authors.forename: K S
authors.initials: KS
authors.lastname: Kim
authors.forename: B K
authors.initials: BK
authors.lastname: Kang
authors.forename: S T
authors.initials: ST
authors.lastname: Park
authors.forename: C H
authors.initials: CH
authors.lastname: Chang
authors.forename: C H
authors.initials: CH
authors.lastname: Kim
authors.forename: Y M
authors.initials: YM
authors.lastname: Lee
authors.forename: H M
authors.initials: HM
authors.lastname: Kim
authors.forename: N H
authors.initials: NH
authors.lastname: An
authors.forename: J J
authors.initials: JJ
authors.lastname: Kim
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D000431:Ethanol / Q000037:antagonists & inhibitors / Q000633:toxicity*; D006801:Humans; D019668:Liliaceae; D008113:Liver Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D010936:Plant Extracts / Q000627:therapeutic use*; D018517:Plant Roots; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D000431:Ethanol