AMP-activated protein kinase counteracted the inhibitory effect of glucose on the phosphoenolpyruvate carboxykinase gene expression in rat hepatocytes.
The effect of AMP-activated protein kinase (AMPK) in the regulation of the phosphoenolpyruvate carboxykinase (PEPCK) gene expression was studied in isolated rat hepatocytes. Activation of AMPK by AICAR counteracted the inhibitory effect of glucose on the PEPCK gene expression, both at the mRNA and the transcriptional levels. It is proposed that a target for AMPK is involved in the inhibitory effect of glucose on PEPCK gene transcription.
Hubert
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Groupe Appareil Digestif Environnement Nutrition, Institut Fédératif de Recherches Multidisciplinaires sur les Peptides no. 23 (IFRMP), UFR Médecine-Pharmacie de Rouen, 22 Boulevard Gambetta, 76183 Cedex, Rouen, France.
Husson
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Chédeville
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Lavoinne
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D055372:AMP-Activated Protein Kinases; D000249:Adenosine Monophosphate / Q000502:physiology*; D000620:Aminoimidazole Carboxamide / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D004789:Enzyme Activation / Q000187:drug effects / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects*; D005947:Glucose / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D007004:Hypoglycemic Agents / Q000494:pharmacology; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D009097:Multienzyme Complexes / Q000502:physiology*; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012265:Ribonucleotides / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects
D007004:Hypoglycemic Agents; D009097:Multienzyme Complexes; D012333:RNA, Messenger; D012265:Ribonucleotides; D000620:Aminoimidazole Carboxamide; D000249:Adenosine Monophosphate; D017346:Protein Serine-Threonine Kinases; D055372:AMP-Activated Protein Kinases; D010729:Phosphoenolpyruvate Carboxykinase (GTP); C031143:AICA ribonucleotide; D005947:Glucose