title: Age dependency of neointima formation on vascular prostheses in dogs.
abstract: Neointima formed quickly on vascular prostheses implanted in young dogs but not in aged dogs. Previously, we found that impeding neointima formation on vascular prostheses occurred more frequently in aged animals. From these observations, we hypothesized that neointima formation was age-dependent in dogs. To test the hypothesis, 26 fabric Dacron vascular prostheses were analyzed. Half of them were retrieved from aged dogs (more than 13 years old) while the other half were from young ones (less than 1 year old). The grafts were harvested at 8 weeks and 3 months after implantation. The graft surfaces were photographed and analyzed by computer for the ratio of the areas with and without thrombus. Light and scanning electron microscopic observation revealed that most of the thrombus-free areas were lined with endothelial cells. Then the endothelialized areas were calculated. Using data obtained from macroscopic, light microscopic, and scanning electron microscopic observations, the arithmetic means were calculated as the degree of neointima formation. In young animals, the degrees at 8 weeks and at 3 months were 89.1 +/- 8.5% (mean +/- SD) and 95.7 +/- 3.3%, respectively. In old animals, they were 27.9 +/- 5.9% and 31.5 +/- 6. 8%, respectively. From these results, we concluded that neointima formation was age-dependent in dogs.
authors.affiliation: Department of Surgery, Yokohama City University, School of Medicine, Yokohama, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Noishiki
authors.forename: Y
authors.initials: Y
authors.lastname: Yamane
authors.forename: Y
authors.initials: Y
authors.lastname: Ichikawa
authors.forename: I
authors.initials: I
authors.lastname: Yamazaki
authors.forename: K
authors.initials: K
authors.lastname: Yamamoto
authors.forename: T
authors.initials: T
authors.lastname: Kosuge
authors.forename: T
authors.initials: T
authors.lastname: Manabe
authors.forename: M
authors.initials: M
authors.lastname: Mo
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D001013:Aorta, Thoracic / Q000601:surgery; D001672:Biocompatible Materials; D001807:Blood Vessel Prosthesis; D019917:Blood Vessel Prosthesis Implantation; D002196:Capillaries / Q000648:ultrastructure; D004396:Coloring Agents; D004285:Dogs; D004730:Endothelium, Vascular / Q000502:physiology / Q000648:ultrastructure; D005347:Fibroblasts / Q000648:ultrastructure; D005456:Fluorescent Dyes; D005500:Follow-Up Studies; D007091:Image Processing, Computer-Assisted; D008855:Microscopy, Electron, Scanning; D009131:Muscle, Smooth, Vascular / Q000502:physiology / Q000648:ultrastructure; D010781:Photography; D011093:Polyethylene Terephthalates; D011474:Prosthesis Design; D013499:Surface Properties; D013927:Thrombosis / Q000473:pathology; D017539:Tunica Intima / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D001672:Biocompatible Materials; D004396:Coloring Agents; D005456:Fluorescent Dyes; D011093:Polyethylene Terephthalates