title: Blue-fluorescent antibodies.
abstract: The forte of catalytic antibodies has resided in the control of the ground-state reaction coordinate. A principle and method are now described in which antibodies can direct the outcome of photophysical and photochemical events that take place on excited-state potential energy surfaces. The key component is a chemically reactive optical sensor that provides a direct report of the dynamic interplay between protein and ligand at the active site. To illustrate the concept, we used a trans-stilbene hapten to elicit a panel of monoclonal antibodies that displayed a range of fluorescent spectral behavior when bound to a trans-stilbene substrate. Several antibodies yielded a blue fluorescence indicative of an excited-state complex or "exciplex" between trans-stilbene and the antibody. The antibodies controlled the isomerization coordinate of trans-stilbene and dynamically coupled this manifold with an active-site residue. A step was taken toward the use of antibody-based photochemical sensors for diagnostic and clinical applications.
authors.affiliation: Department of Chemistry, The Scripps Research Institute and the Skaggs Institute for Chemical Biology, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
authors.forename: A
authors.initials: A
authors.lastname: Simeonov
authors.forename: M
authors.initials: M
authors.lastname: Matsushita
authors.forename: E A
authors.initials: EA
authors.lastname: Juban
authors.forename: E H
authors.initials: EH
authors.lastname: Thompson
authors.forename: T Z
authors.initials: TZ
authors.lastname: Hoffman
authors.forename: A E
authors.initials: AE
authors.lastname: Beuscher
authors.forename: M J
authors.initials: MJ
authors.lastname: Taylor
authors.forename: P
authors.initials: P
authors.lastname: Wirsching
authors.forename: W
authors.initials: W
authors.lastname: Rettig
authors.forename: J K
authors.initials: JK
authors.lastname: McCusker
authors.forename: R C
authors.initials: RC
authors.lastname: Stevens
authors.forename: D P
authors.initials: DP
authors.lastname: Millar
authors.forename: P G
authors.initials: PG
authors.lastname: Schultz
authors.forename: R A
authors.initials: RA
authors.lastname: Lerner
authors.forename: K D
authors.initials: KD
authors.lastname: Janda
mesh_terms: D017151:Antibodies, Catalytic / Q000737:chemistry*; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D001665:Binding Sites; D001666:Binding Sites, Antibody; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D018360:Crystallography, X-Ray; D005453:Fluorescence; D006241:Haptens; D008024:Ligands; D008856:Microscopy, Fluorescence; D008956:Models, Chemical; D008958:Models, Molecular; D010777:Photochemistry; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism; D013267:Stilbenes / Q000737:chemistry* / Q000276:immunology*; D013696:Temperature; D014466:Ultraviolet Rays
chemical_list: D017151:Antibodies, Catalytic; D000911:Antibodies, Monoclonal; D006241:Haptens; D008024:Ligands; D013267:Stilbenes