Heat shock protein 60 sequence comparisons: duplications, lateral transfer, and mitochondrial evolution.
Heat shock proteins 60 (GroEL) are highly expressed essential proteins in eubacterial genomes and in eukaryotic organelles. These chaperone proteins have been advanced as propitious marker sequences for tracing the evolution of mitochondrial (Mt) genomes. Similarities among HSP60 sequences based on significant segment pair alignment calculations are used to deduce associations of sequences taking into account GroEL functional/structural domain differences and to relate HSP60 duplications pervasive in alpha-proteobacterial lineages to the dynamics of lateral transfer and plasmid integration. Multiple alignments with consensuses are determined for 10 natural groups. The group consensuses sharpen the similarity contrasts among individual sequences. In particular, the Mt group matches best with the classical alpha-proteobacteria and closely with Rickettsia but significantly worse with the rickettsial groups Ehrlichia and Orientia. However, across broad protein sequence comparisons, there appears to be no consistent prokaryote whose protein sequences align best with animal Mt genomes. There are plausible scenarios indicating that the nuclear-encoded HSP60 (and HSP70) sequences functioning in Mt are results of lateral transfer and are probably derived from an alpha-proteobacterium. This hypothesis relates to the plethora of duplicated HSP60 sequences among the classical alpha-proteobacteria contrasted with no duplications of HSP60 among other clades of proteobacterial genomes. Evolutionary relations are confounded by differential selection pressures, convergence, variable mutational rates, site variability, and lateral gene transfer.
Karlin
S
S
Department of Mathematics, Stanford University, Stanford, CA 94305-2125, USA. karlin@math.stanford.edu
Brocchieri
L
L
D001419:Bacteria / Q000235:genetics; D018834:Chaperonin 60 / Q000235:genetics*; D019143:Evolution, Molecular; D005658:Fungi / Q000235:genetics; D020440:Gene Duplication; D018014:Gene Transfer Techniques; D008928:Mitochondria / Q000235:genetics*; D010944:Plants / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D018834:Chaperonin 60