title: Reduced levels of 1,25-dihydroxyvitamin D(3) in rat dams and offspring after exposure to a reconstituted PCB mixture.
abstract: Previous studies revealed effects of polychlorinated biphenyls (PCBs) and other polyhalogenated hydrocarbons on steroid hormone levels and hormone-dependent functions including behavior. In the present study serum concentrations of the vitamin D(3) metabolites 25-hydroxycholecalciferol (25-D) and 1,25-dihydroxycholecalciferol (1,25-D) were determined in rat dams and offspring after exposure to a PCB mixture that was reconstituted according to the congener pattern found in human breast milk. Unmated females were exposed to diets adulterated with 0; 5; 20; or 40 mg PCBs/kg diet. Exposure started 50 days prior to mating and was terminated at birth. Gestational exposure reduced serum concentrations of 1,25-D in dams in a dose-dependent manner. Concentration of 25-D was also decreased at the time of delivery, but not at weaning. Determination of 1,25-D in offspring at weaning revealed reductions in both high-exposure groups. Levels of 25-D were diminished only at the highest exposure level. Internal PCB concentrations in adipose tissue and brains exhibited a linear relation to dosages in diet. Concentrations of PCBs in brains were similar in dams and offspring at birth, but decreased at the end of lactation in dams. In offspring, values increased during this period because of continued exposure via the milk. In the adipose tissue, PCB levels were much lower in offspring than in dams. To our knowledge, this is the first report of PCB-induced effects on vitamin D(3) metabolites. In dams, reductions were seen even at the lowest exposure level used. Further studies are needed to evaluate the biological significance of these reductions in pregnant dams and possible consequences for the developing offspring.
authors.affiliation: Departments of Neurobehavioral Toxicology and Analytical Chemistry, Medical Institute of Environmental Hygiene, Auf'm Hennekamp 50, D-404225 Düsseldorf, Germany. lilien@rz.uni-duesseldorf.de
authors.forename: H
authors.initials: H
authors.lastname: Lilienthal
authors.forename: A
authors.initials: A
authors.lastname: Fastabend
authors.forename: J
authors.initials: J
authors.lastname: Hany
authors.forename: H
authors.initials: H
authors.lastname: Kaya
authors.forename: A
authors.initials: A
authors.lastname: Roth-Härer
authors.forename: L
authors.initials: L
authors.lastname: Dunemann
authors.forename: G
authors.initials: G
authors.lastname: Winneke
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn / Q000097:blood*; D000833:Animals, Suckling / Q000097:blood*; D001921:Brain / Q000378:metabolism; D002112:Calcifediol / Q000097:blood; D002117:Calcitriol / Q000097:blood*; D004032:Diet; D004305:Dose-Response Relationship, Drug; D005314:Embryonic and Fetal Development / Q000187:drug effects; D004785:Environmental Pollutants / Q000008:administration & dosage / Q000633:toxicity*; D005260:Female; D008297:Male; D018811:Maternal Exposure; D011078:Polychlorinated Biphenyls / Q000008:administration & dosage / Q000633:toxicity*; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000097:blood* / Q000187:drug effects; D051381:Rats; D020318:Rats, Long-Evans; D012098:Reproduction / Q000187:drug effects
chemical_list: D004785:Environmental Pollutants; D011078:Polychlorinated Biphenyls; D002117:Calcitriol; D002112:Calcifediol