Endogenous fatty acid synthesis in squamous cell carcinomas of the oral cavity.
We measured the synthesis of endogenous fatty acids in oral squamous cell carcinoma, its adjacent tissue, and normal oral tissues that were collected fresh from surgical specimens. The tissues were minced in Roswell Park Memorial Institute Medium 1640 (RPMI 1640) and incubated in [1(2)-(14)C] acetic acid, sodium salt ((14)CH(3)(14)CO(2)Na). Total lipids were then extracted and(14)C was measured by scintillation counter. We found that incorporation of(14)C in the total lipids of squamous cell carcinoma was significantly higher than in the other tissues.
Guo
C B
CB
Department of Oral and Maxillofacial Surgery, School of Stomatology, Beijing Medical University, Beijing, People's Republic of China. guodazuo@263.net
Cui
L H
LH
Yu
G Y
GY
Liu
D X
DX
Meng
S C
SC
Song
Q
Q
D002250:Carbon Radioisotopes; D002294:Carcinoma, Squamous Cell / Q000737:chemistry / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis / Q000096:biosynthesis*; D006801:Humans; D009061:Mouth Mucosa / Q000737:chemistry / Q000378:metabolism; D009062:Mouth Neoplasms / Q000737:chemistry / Q000378:metabolism*; D012588:Scintillation Counting
D002250:Carbon Radioisotopes; D005227:Fatty Acids