[Features of the development of homo- and heterotopic allotransplants of rat embryonal neocortex].
Mechanisms of regulation of cell division in the developing neocortex are largely unknown. The aim of the present study was to investigate the influence of a microenvironment on the fetal neocortex histogenesis. The fetal neocortex from 15-day old Wistar rat embryo was grafted into the neocortex, crushed sciatic nerve and anterior chamber of eye of adult rats. A comparative study of graft development was carried out on 1, 3, 7, 10, 30 days using histological (Nissl stain, hematoxylin-eosin) and immunohistochemical (monoclonal antibody to proliferating cell nuclear antigen, and to glial fibrillary acidic protein) methods. Grafted neuroepithelial cells proliferated in grafts that developed in the neocortex and the anterior chamber of eye for 7 days, and in the sciatic nerve for 10 days. In all grafts differentiating neuroblasts, young neurons and mature neurons were observed 7, 10 and 30 days later, respectively. In 10 days, transplants in the nerve have a glial capsule, in contrast to other sites of grafting. The capsule consists of ependymocytes with microvilli and cilia 30 days later. These cells are GFAP-positive. Our results indicate epigenetic influence on the development of neuroepithelial precursors. The microenvironment of the peripheral nerve is suggested to promote glyogenesis in developing grafts. Afferent inputs do not influence the proliferative potency of brain cell precursors.
Petrova
E S
ES
Institute of Experimental Medicine RAMS, St. Petersburg.
Otellin
V A
VA
D000818:Animals; D000869:Anterior Eye Segment; D002455:Cell Division; D016332:Fetal Tissue Transplantation; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D019579:Neocortex / Q000166:cytology / Q000196:embryology / Q000378:metabolism / Q000637:transplantation*; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve; D014184:Transplantation, Homologous
D005904:Glial Fibrillary Acidic Protein