title: [Dependence of the kinetics of forming phases of a two-phase polymeric system on actin state].
abstract: New analytical possibilities of biological macromolecule partition in a two-phase polymer system (dextran-500/polyethyleneglycol-6000) were investigated. The technical principles were based on the fact that optical density of the system changes during phase formation. Various actin forms were investigated. Unlike G-actin, F-actin increases the speed of phase formation. Different actin isoforms exert different influence on this process. The kinetics of the partition system into phases makes it possible to figure out the picture of native G-actin damage. The two-phase polymer system has been shown to be capable of supporting the native properties of actin for not less than 6 days at 20 degrees C. An assumption is made that two-phase systems can be used for imitating the intracellular medium in studies on actin functional characteristics.
authors.affiliation: Institute of Cytology RAS, St. Petersburg.
authors.forename: Iu P
authors.initials: IuP
authors.lastname: Petrov
authors.forename: G P
authors.initials: GP
authors.lastname: Pinaev
authors.forename: I E
authors.initials: IE
authors.lastname: Stepanian
authors.forename: S Iu
authors.initials: SIu
authors.lastname: Khaĭtlina
mesh_terms: D000199:Actins / Q000737:chemistry*; D003911:Dextrans / Q000737:chemistry*; D007700:Kinetics; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*
chemical_list: D000199:Actins; D003911:Dextrans; D011108:Polymers; D011092:Polyethylene Glycols