Determination of cefditoren (ME 1206) in the plasma of elderly patients with multiple diseases using high-performance liquid chromatography.
In the present study we developed a new sensitive and selective HPLC-assay for the determination of cefditoren (ME 1206) in the plasma. The column switching technique was used to regenerate the pre-column and the analytical column separately. This method is suitable for pharmacokinetic studies and for drug monitoring. Thus, we measured plasma samples of 15 elderly patients (age range: 67-88 years), receiving cefditoren-pivoxil therapy because of infections of the respiratory tract. In addition, these patients were treated with several co-medications because of further diseases. With our HPLC-method no interfering peaks could be detected at the retention time of cefditoren (10.67 min) or of the internal standard (16.62 min). The lowest plasma concentration of cefditoren to be quantified was 25 ng/ml (R.S.D.= 15.5%, n = 5).
Rieck
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Institute of Gerontology-Chair of Internal Medicine, University of Erlangen-Nuremberg, Germany.
Platt
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D000368:Aged; D000369:Aged, 80 and over; D002138:Calibration; D002511:Cephalosporins / Q000097:blood* / Q000493:pharmacokinetics / Q000627:therapeutic use; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D016903:Drug Monitoring / Q000295:instrumentation / Q000379:methods; D004359:Drug Therapy, Combination; D004867:Equipment Design; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results; D012141:Respiratory Tract Infections / Q000097:blood* / Q000188:drug therapy; D012680:Sensitivity and Specificity
D002511:Cephalosporins; C073460:cefditoren