title: Rapidly turned over protein maintains metabotropic synaptic transmission in Purkinje cells.
abstract: It has generally been thought that protein synthesis is required for relatively slow cellular processes such as synaptogenesis and synaptic plasticity. In this study on rat cerebellar slices, we found that metabotropic glutamate receptor-mediated synaptic transmission to cerebellar Purkinje cells was quickly and persistently depressed by brief (5 min) applications of translational inhibitors, which were confirmed to induce quick and persistent depression of protein synthesis in cerebellar tissues. Brief applications of transcriptional inhibitors also depressed metabotropic synaptic transmission, but progressively over 1 h, presumably due to depletion of mRNAs in the dendrites. Results of this study indicate the presence of a unique protein(s) that is dynamically involved in metabotropic synaptic transmission.
authors.affiliation: Brain Science Institute, Institute of Physical and Chemical Research (RIKEN), Wako, Saitama, Japan.
authors.forename: L
authors.initials: L
authors.lastname: Karachot
authors.forename: Y
authors.initials: Y
authors.lastname: Shirai
authors.forename: R
authors.initials: R
authors.lastname: Vigot
authors.forename: T
authors.initials: T
authors.lastname: Yamamori
authors.forename: M
authors.initials: M
authors.lastname: Ito
mesh_terms: D000818:Animals; D000841:Anisomycin / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004004:Dichlororibofuranosylbenzimidazole / Q000494:pharmacology; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects / Q000502:physiology; D008715:Methionine / Q000493:pharmacokinetics; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000187:drug effects; D009924:Organ Culture Techniques; D014176:Protein Biosynthesis / Q000187:drug effects / Q000502:physiology; D011689:Purkinje Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D011691:Puromycin / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D018094:Receptors, Metabotropic Glutamate / Q000187:drug effects / Q000378:metabolism*; D013462:Sulfur Radioisotopes; D013569:Synapses / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology*; D014158:Transcription, Genetic / Q000187:drug effects / Q000502:physiology
chemical_list: D009419:Nerve Tissue Proteins; D018094:Receptors, Metabotropic Glutamate; D013462:Sulfur Radioisotopes; D003609:Dactinomycin; D011691:Puromycin; D004004:Dichlororibofuranosylbenzimidazole; D000841:Anisomycin; D003513:Cycloheximide; D008715:Methionine