title: Use of combined microautoradiography and fluorescence in situ hybridization to determine carbon metabolism in mixed natural communities of uncultured bacteria from the genus Achromatium.
abstract: Combined microautoradiography and fluorescence in situ hybridization (FISH) was used to investigate carbon metabolism in uncultured bacteria from the genus Achromatium. All of the Achromatium species identified in a freshwater sediment from Rydal Water, Cumbria, United Kingdom, which were distinguishable only by FISH, assimilated both [(14)C]bicarbonate and [(14)C]acetate. This extends previous findings that Achromatium spp. present at another location could only utilize organic carbon sources. Achromatium spp., therefore, probably exhibit a range of physiologies, i.e., facultative chemolithoautotrophy, mixotrophy, and chemoorganoheterotrophy, similar to other large sulfur bacteria (e.g., Beggiatoa spp.).
authors.affiliation: Fossil Fuels and Environmental Geochemistry Postgraduate Institute and Centre for Molecular Ecology, University of Newcastle, Newcastle upon Tyne NE1 7RU, United Kingdom.
authors.forename: N D
authors.initials: ND
authors.lastname: Gray
authors.forename: R
authors.initials: R
authors.lastname: Howarth
authors.forename: R W
authors.initials: RW
authors.lastname: Pickup
authors.forename: J G
authors.initials: JG
authors.lastname: Jones
authors.forename: I M
authors.initials: IM
authors.lastname: Head
mesh_terms: D000085:Acetates / Q000378:metabolism*; D001345:Autoradiography / Q000379:methods; D001639:Bicarbonates / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism*; D002250:Carbon Radioisotopes; D005618:Fresh Water / Q000382:microbiology*; D019015:Geologic Sediments / Q000382:microbiology*; D006088:Gram-Negative Aerobic Bacteria / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism*; D017404:In Situ Hybridization, Fluorescence; D010802:Phylogeny
chemical_list: D000085:Acetates; D001639:Bicarbonates; D002250:Carbon Radioisotopes; D002244:Carbon