Detection of new propofol metabolites in human urine using gas chromatography/mass spectrometry and liquid chromatography/mass spectrometry techniques.
Using hyphenated analytical techniques, gas chromatography/mass spectrometry (GC/MS) and liquid chromatography/mass spectrometry (LC/MS), a study on minor propofol metabolites in human urine was conducted. These techniques allowed identification of two new phase I metabolites (2-(omega-propanol)-6-isopropylphenol and 2-(omega-propanol)-6-isopropyl-1,4-quinol). In addition, their four corresponding conjugates (three glucuronides and one sulphate) were detected. Thus in human urine at least eight conjugate metabolites are produced, derived from four different aglycones (propofol; 2, 6-diisopropyl-1,4-quinol; 2-(omega-propanol)-6-isopropylphenol and 2-(omega-propanol)-6-isopropyl-1,4-quinol).
Favetta
P
P
Département de Pharmacie Clinique, de Pharmacocinétique et d'Evaluation du Médicament, Institut des Sciences Pharmaceutiques et Biologiques de Lyon, 8 avenue Rockefeller, 69373 Lyon Cedex 08, France.
Dufresne
C
C
Désage
M
M
Païssé
O
O
Perdrix
J P
JP
Boulieu
R
R
Guitton
J
J
D018686:Anesthetics, Intravenous / Q000493:pharmacokinetics / Q000652:urine*; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D015742:Propofol / Q000493:pharmacokinetics / Q000652:urine*
D018686:Anesthetics, Intravenous; D007202:Indicators and Reagents; D015742:Propofol