Molecular evolution in the hypervariable regions of fetuin: comparison between human and African green monkey fetuin.
Sequences of fetuin cDNA and its deduced amino acid residues from the African green monkey cell line Vero were found to differ by 7.3% and 12.9%, respectively, from the corresponding human sequences. Most amino acid substitutions were clustered within a small segment of the third domain (D3). Calculations of nonsynonymous and synonymous nucleotide substitution rates suggest that this small segment was mutated under positive selection. cDNAs encoding alpha1-antitrypsin, beta-actin and the sequences of intron 4 of alpha1-antitrypsin gene in human liver and Vero cells were also investigated. The results substantiated the positive selection imposed on the D3 segment.
Yoshida
K
K
Department of Biochemistry, Kinki University School of Medicine, Osaka-Sayama, Osaka, Japan.
Suzuki
Y
Y
Yamamoto
K
K
Matsuura
K
K
Watanabe
M
M
Sinohara
H
H
D000199:Actins / Q000235:genetics; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D001483:Base Sequence; D002522:Chlorocebus aethiops; D022801:Complementarity Determining Regions / Q000737:chemistry* / Q000235:genetics; D003198:Computer Simulation; D018076:DNA, Complementary / Q000235:genetics; D019143:Evolution, Molecular; D006801:Humans; D007438:Introns; D008099:Liver / Q000201:enzymology; D008954:Models, Biological; D008969:Molecular Sequence Data; D009154:Mutation; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014709:Vero Cells / Q000737:chemistry; D000515:alpha 1-Antitrypsin / Q000235:genetics; D000509:alpha-Fetoproteins / Q000737:chemistry* / Q000235:genetics
D000199:Actins; D022801:Complementarity Determining Regions; D018076:DNA, Complementary; D000515:alpha 1-Antitrypsin; D000509:alpha-Fetoproteins