title: Effects of hypoxemia on the a- and b-waves of the electroretinogram in the cat retina.
abstract: OBJECTIVE
  Slow components of the electroretinogram (ERG) are sensitive to even mild hypoxemia (60 < P(a)O(2) < 100 mm Hg) in the cat eye. However, the electrical responses of the inner retina remain unchanged until P(a)O(2) is below 40 mm Hg. In this study, the effects of hypoxemia on photoreceptors, on which both slow ERG components and inner retinal activity depend, were examined by recording the a-wave of the ERG.
  
  
  METHODS
  The ERG of dark-adapted, anesthetized cats was recorded between an Ag-AgCl electrode in the vitreous humor and a reference electrode near the eye. Responses to bright flashes of diffuse white light were recorded at 3-minute intervals during hypoxemic episodes lasting 15 minutes to 2 hours.
  
  
  RESULTS
  The cat a-wave was well described by the Lamb and Pugh a-wave model during normoxia and hypoxemia. During mild hypoxemia (P(a)O(2) of 50-60 mm Hg), small changes in a-wave amplitude were detected but did not become greater during severe hypoxemia. The mean decrease in the a-wave amplitude during severe hypoxemia (P(a)O(2) of 20-30 mm Hg) was 8.9% from the mean amplitude during air breathing. The effects of hypoxemia were more severe on the b-wave amplitude. The mean decrease in the b-wave was 35% at P(a)O(2) of 20-30 mm Hg.
  
  
  CONCLUSIONS
  The a-wave is more resistant to severe hypoxemia than the b-wave. This implies that photoreceptor transduction works almost normally during hypoxemia and that failure of inner retinal PO(2) regulation causes the decrease in the b-wave. Previously observed changes in the amplitudes of slow ERG components during hypoxemia may result from changes in the ionic environment, rather than a failure of photoreceptor energy metabolism.
authors.affiliation: Department of Biomedical Engineering, Northwestern University, Evanston, Illinois 60208-3107, USA.
authors.forename: J
authors.initials: J
authors.lastname: Kang Derwent
authors.forename: R A
authors.initials: RA
authors.lastname: Linsenmeier
mesh_terms: D000818:Animals; D002415:Cats; D003623:Dark Adaptation; D004596:Electroretinography; D000860:Hypoxia / Q000503:physiopathology*; D010775:Photic Stimulation; D020419:Photoreceptor Cells, Vertebrate / Q000502:physiology*; D014785:Vision, Ocular / Q000502:physiology