Inducible stx2 phages are lysogenized in the enteroaggregative and other phenotypic Escherichia coli O86:HNM isolated from patients.
We characterized two Shiga toxin-producing Escherichia coli (STEC) O86:HNM isolates from a patient with hemolytic uremic syndrome (HUS) or bloody diarrhea. Both of them did not possess the eaeA gene. However, the isolate from a HUS patient carried genetic markers of enteroaggregative E. coli (EAEC) and showed aggregative adherence pattern to HEp-2 cells. The other isolate from bloody diarrhea, which was negative with EAEC markers, was diffusely adhered to HEp-2 cells. The stx2 gene in both E. coli O86:HNM strains was encoded in each infectious phage, which was partially homologous to that of strain EDL933, a STEC O157:H7. These results will help to explain the genotypic divergences of STEC.
Iyoda
S
S
Department of Bacteriology, National Institute of Infectious Diseases, Tokyo, Japan.
Tamura
K
K
Itoh
K
K
Izumiya
H
H
Ueno
N
N
Nagata
K
K
Togo
M
M
Terajima
J
J
Watanabe
H
H
D001422:Bacterial Adhesion; D015139:Blotting, Southern; D002478:Cells, Cultured; D003090:Coliphages / Q000235:genetics*; D003967:Diarrhea / Q000382:microbiology; D004926:Escherichia coli / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism / Q000472:pathogenicity* / Q000821:virology; D004927:Escherichia coli Infections / Q000382:microbiology*; D006463:Hemolytic-Uremic Syndrome / Q000382:microbiology; D006801:Humans; D008242:Lysogeny; D010641:Phenotype; D016133:Polymerase Chain Reaction; D022641:Shiga Toxin 2 / Q000378:metabolism*; D014774:Virulence
D022641:Shiga Toxin 2