Selective enrichment broth for the isolation of Aeromonas sp. from chicken meat.
Six selective agents (ampicillin, novobiocin, cephalothin, bile salts, brilliant green and ethanol) were tested during the development of a selective enrichment broth for the isolation of Aeromonas sp. from food. Cephalothin at 10 mg/l was found to be the best selective agent owing to its greater selectivity and efficiency in recovering stressed and lower cell concentrations of Aeromonas sp. Higher concentrations (15-25 mg/l) of cephalothin were inhibitory to some strains of A. sobria. Cephalothin (10 mg/l) was incorporated in buffered dextrin broth (BCDB-10) and alkaline peptone water (CAPW-10) and employed for the isolation of Aeromonas sp. from chicken meat naturally and artificially inoculated (with 10(9) cells/ml of A. hydrophila). The highest isolation rate (22%) with naturally contaminated chicken was achieved with CAPW-10 in comparison to 16% with BCDB-10 and 8% with APW. Similarly, from artificially inoculated samples, 100% isolation was accomplished with CAPW-10, against 80% with BCDB-10 and 50% with APW.
Sachan
N
N
FAO/WHO Collaborating Centre for Research & Training in Veterinary Public Health, Division of Veterinar, Public Health, Indian Veterinary Research Institute, Izatnagar, Bareilly.
Agarwal
R K
RK
D000333:Aeromonas / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification*; D000667:Ampicillin / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001647:Bile Acids and Salts / Q000494:pharmacology; D002512:Cephalothin / Q000494:pharmacology; D002645:Chickens; D017077:Culture Media, Conditioned; D000431:Ethanol / Q000494:pharmacology; D005516:Food Microbiology; D008460:Meat / Q000382:microbiology*; D009675:Novobiocin / Q000494:pharmacology; D013997:Time Factors
D000900:Anti-Bacterial Agents; D001647:Bile Acids and Salts; D017077:Culture Media, Conditioned; D009675:Novobiocin; D000431:Ethanol; D000667:Ampicillin; D002512:Cephalothin