Detection of IL-2 and IFN-gamma m RNA expression in bovine milk cells at the late stage of the lactation period with RT-PCR.
The expression of bovine interferon-gamma (IFN-gamma) and interleukin-2 (IL-2) in the late stage of the lactation period in milk cells was detected with reverse transcriptase-polymerase chain reaction (RT-PCR). IFN-gamma was detected with all primer concentrations, 0.2, 0.5 and 1 microM. IL-2 however, was faintly detected only with 0.5 microM primer concentration. It was shown that IFN-gamma m RNA expression can be demonstrated clearly with RT-PCR at the late stage of the lactation period. In contrast, IL-2 was weakly expressed at this stage of the lactation period. The work is important for substantiating the therapeutic use of recombinant bovine IFN-gamma and IL-2 and for helping to reveal the activity of cytokine in the mammary gland at the late stage of the lactation period.
Alluwaimi
A M
AM
Department of Microbiology and Parasitology, College of Veterinary Medicine and Animal Resources, King Faisal University, P.O. Box 1757, Hofuf 31982, Saudi Arabia.
D000818:Animals; D002417:Cattle / Q000378:metabolism*; D017931:DNA Primers / Q000378:metabolism; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D005260:Female; D007371:Interferon-gamma / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000096:biosynthesis*; D007774:Lactation; D008892:Milk / Q000737:chemistry / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary*; D013997:Time Factors
D017931:DNA Primers; D007376:Interleukin-2; D012333:RNA, Messenger; D007371:Interferon-gamma