Effect of high concentrations of sucrose on the enzymatic activity of alpha-chymotrypsin.
The effect of the low-molecular-mass natural reagents in high concentrations is important for investigating enzymatic reactions in near "in vivo" conditions and for optimisation of biotechnology processes. A model system, including p-nitrophenyl acetate as substrate and alpha-chymotrypsin as proteolytic enzyme, has been used to study the effect of high concentrations of sucrose, both influencing the viscosity of the reaction medium and acting as a nucleophilic effector (activator) on the enzymatic reaction. A kinetic scheme at high concentrations of nucleophilic effectors (sucrose) has been proposed.
Todorova
R
R
Institute of Biophysics, Bulgarian Academy of Sciences, Sofia. todorova@obzor.bio21.bas.bg
Nedev
K
K
D000818:Animals; D002417:Cattle; D002918:Chymotrypsin / Q000378:metabolism*; D006868:Hydrolysis; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D007700:Kinetics; D009596:Nitrophenols / Q000378:metabolism; D013379:Substrate Specificity; D013395:Sucrose / Q000494:pharmacology*
D007202:Indicators and Reagents; D009596:Nitrophenols; D013395:Sucrose; C008642:4-nitrophenyl acetate; D002918:Chymotrypsin; C117608:alpha-chymotrypsin