Association study on the DUSP6 gene, an affective disorder candidate gene on 12q23, performed by using fluorescence resonance energy transfer-based melting curve analysis on the LightCycler.
We introduced a new genotyping method, fluorescence resonance energy transfer-based melting curve analysis on the LightCycler, for the analysis of the gene, DUSP6 (dual specificity MAP kinase phosphatase 6), in affective disorder patients. The DUSP6 gene is located on chromosome 12q22-23, which overlaps one of the reported bipolar disorder susceptibility loci. Because of its role in intracellular signalling pathways, the gene may be involved in the pathogenesis of affective disorders not only on the basis of its position but also of its function. We performed association analysis using a T>G polymorphism that gives rise to a missense mutation (Leu114Val). No evidence for a significant disease-causing effect was found in Japanese unipolars (n = 132) and bipolars (n = 122), when compared with controls (n = 299). More importantly, this study demonstrates that melting curve analysis on the LightCycler is an accurate, rapid and robust method for discriminating genotypes from biallelic markers. This strategy has the potential for use in high throughput scanning for and genotyping of single nucleotide polymorphisms (SNPs).
Toyota
T
T
Laboratory for Molecular Psychiatry, Brain Science Institute, RIKEN, Wako, Saitama, Japan.
Watanabe
A
A
Shibuya
H
H
Nankai
M
M
Hattori
E
E
Yamada
K
K
Kurumaji
A
A
Karkera
J D
JD
Detera-Wadleigh
S D
SD
Yoshikawa
T
T
D000328:Adult; D000368:Aged; D000483:Alleles; D001714:Bipolar Disorder / Q000235:genetics*; D002881:Chromosomes, Human, Pair 12; D004252:DNA Mutational Analysis / Q000379:methods; D003866:Depressive Disorder / Q000235:genetics; D054642:Dual Specificity Phosphatase 6; D005260:Female; D005820:Genetic Testing / Q000379:methods*; D005838:Genotype; D006801:Humans; D007564:Japan; D008297:Male; D008875:Middle Aged; D020125:Mutation, Missense; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D017027:Protein Tyrosine Phosphatases / Q000235:genetics*; D013050:Spectrometry, Fluorescence
C517035:DUSP6 protein, human; D054642:Dual Specificity Phosphatase 6; D017027:Protein Tyrosine Phosphatases