title: Phagocytosis, oxidative burst, and killing of Candida dubliniensis and Candida albicans by human neutrophils.
abstract: Candida dubliniensis is a phylogenetically closely related species to Candida albicans. So far virtually nothing is known about the virulence factors of C. dubliniensis. Cell surface hydrophobicity (CSH) plays a critical role in adhesion of microorganisms to phagocytic cells; hydrophobic cells of C. albicans have been reported to be less sensitive to phagocytic killing than hydrophilic cells. C. dubliniensis displays CSH at 37 degrees C in contrast to C. albicans. To elucidate this issue, we determined levels of phagocytosis, oxidative burst and killing by human neutrophils of C. dubliniensis (n=10) compared to C. albicans (n=10) both cultured at 37 degrees C. Obtained test results revealed no statistically significant differences between these two yeast species for the level of phagocytosis (77.3 vs. 76.2% after 60 min), evoked oxidative burst (64.5 vs. 67.3% after 30 min) and killing (72.7 vs. 73.1% after 240 min). Therefore, human neutrophils can be considered to be equally efficient against these two yeast species.
authors.affiliation: Institute of Medical Microbiology, University Hospital, Aachen, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Peltroche-Llacsahuanga
authors.forename: N
authors.initials: N
authors.lastname: Schnitzler
authors.forename: S
authors.initials: S
authors.lastname: Schmidt
authors.forename: K
authors.initials: K
authors.lastname: Tintelnot
authors.forename: R
authors.initials: R
authors.lastname: Lütticken
authors.forename: G
authors.initials: G
authors.lastname: Haase
mesh_terms: D002175:Candida / Q000276:immunology*; D002176:Candida albicans / Q000276:immunology*; D002177:Candidiasis / Q000382:microbiology; D006801:Humans; D009504:Neutrophils / Q000276:immunology*; D010587:Phagocytosis; D016897:Respiratory Burst