In vitro selected oligonucleotides as receptors in binding assays.
This paper describes a new binding assay that uses oligodeoxyribonucleotides (DNAs) obtained by the in vitro selection method instead of antibodies. DNAs that specifically bound to a target molecule were selected, labeled with some probes, and then used to detect the target molecule by staining or competitive binding assay.
Ito
Y
Y
Department of Biological Science and Technology, Tokushima University, Minami-Josanjima, Tokushima, 770-8506, Japan.
Kawazoe
N
N
Imanishi
Y
Y
D001483:Base Sequence; D001667:Binding, Competitive; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005492:Folic Acid / Q000032:analysis / Q000378:metabolism; D015723:Gene Library; D005818:Genetic Engineering / Q000379:methods; D008024:Ligands; D008727:Methotrexate / Q000032:analysis / Q000378:metabolism; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000378:metabolism*; D012313:RNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013194:Staining and Labeling; D013974:Thyroxine / Q000032:analysis / Q000378:metabolism
D008024:Ligands; D009841:Oligonucleotides; D004364:Pharmaceutical Preparations; D012313:RNA; D004247:DNA; D005492:Folic Acid; D013974:Thyroxine; D008727:Methotrexate