Simultaneous determination of unbound cefoperazone in rat blood and brain using microdialysis.
A sensitive microbore HPLC method was developed for the simultaneous determination of unbound cefoperazone in rat blood and brain using microdialysis. Two microdialysis probes were inserted into the jugular vein/right atrium and brain striatum of Sprague-Dawley rats. Cefoperazone (50 mgkg(-1), i.v.) was then administered via the femoral vein. Blood and brain dialysates were collected and eluted with a mobile phase containing methanol-100 mM monosodium phosphoric acid (30:70, v/v, pH 5.5). The wavelength of the UV detector was set at 254 nm. The detection limit of cefoperazone was 20 ng mL(-1). Isocratic separation of cefoperazone was achieved within 10 min. The intra- and inter-assay accuracy and precision of the analyses were < or =10% in the range of 0.05-10 microg mL(-1). The ratio of the area under the concentration curve of cefoperazone in rat brain and blood was estimated to be about 7-8%. It is concluded that cefoperazone is capable of penetrating the blood-brain barrier.
Chang
Y L
YL
Institute of Pharmacology, National Yang-Ming University, Taipei, Taiwan.
Chou
M H
MH
Lin
M F
MF
Chen
Y F
YF
Chen
C F
CF
Cheng
F C
FC
Tsai
T H
TH
D000818:Animals; D019540:Area Under Curve; D001812:Blood-Brain Barrier; D001921:Brain / Q000378:metabolism*; D002438:Cefoperazone / Q000097:blood / Q000493:pharmacokinetics*; D002511:Cephalosporins / Q000097:blood / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid; D007275:Injections, Intravenous; D008297:Male; D017551:Microdialysis; D051381:Rats; D017207:Rats, Sprague-Dawley
D002511:Cephalosporins; D002438:Cefoperazone