title: Histone deacetylase inhibitors and cell proliferation in pea root meristems.
abstract: The histone deacetylase (HDA) inhibitors, trichostatin A (TSA) and HC toxin halt mitosis in cultured root meristems of Pisnum sativum, while the anti-protozoan HDA inhibitor apicidin is ineffective. Two-dimensional PAGE of proteins from root meristems exposed to TSA and HC toxin did not show significant differences compared to controls, although a previously tested HDA inhibitor, butyrate, exhibited dramatic variations in its protein profile. Northern analysis of butyrate- and TSA-treated root meristems indicated that non-proliferating cells are expressing significant amounts of transcripts of the known cell proliferation associated genes: histone H2A, MAP kinase, cycA2:1 and cdc2. Western analysis reveals the presence of hyperacetylated nuclear proteins in HDA-inhibitor treated cells. These results suggest that the HDA inhibitors, butyrate and TSA, halt mitosis without down-regulating genes that typically have low or nonexistent expression levels in non-dividing cells.
authors.affiliation: Laboratory of Plant Morphogenesis, Manhattan College, Riverdale, NY 10471, USA.
authors.forename: J P
authors.initials: JP
authors.lastname: Murphy
authors.forename: J P
authors.initials: JP
authors.lastname: McAleer
authors.forename: A
authors.initials: A
authors.lastname: Uglialoro
authors.forename: J
authors.initials: J
authors.lastname: Papile
authors.forename: J
authors.initials: J
authors.lastname: Weniger
authors.forename: F
authors.initials: F
authors.lastname: Bethelmie
authors.forename: W A
authors.initials: WA
authors.lastname: Tramontano
mesh_terms: D002455:Cell Division / Q000187:drug effects*; D015180:Electrophoresis, Gel, Two-Dimensional; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D056572:Histone Deacetylase Inhibitors; D018532:Pisum sativum / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D018517:Plant Roots / Q000166:cytology / Q000187:drug effects / Q000201:enzymology
chemical_list: D004791:Enzyme Inhibitors; D056572:Histone Deacetylase Inhibitors