title: Vitellogenin of Blattella germanica (L.) (Dictyoptera, blattellidae): nucleotide sequence of the cDNA and analysis of the protein primary structure.
abstract: The cloning and sequencing of a cDNA of the vitellogenin gene from the cockroach Blattella germanica is reported. It is 5,749 nucleotides long and encodes an amino acid sequence of 1,862 residues (including a putative signal peptide of 17 residues). The vitellogenin sequence includes a long serine-rich stretch between amino acids 322 and 349, and two other stretches between amino acids 1691 and 1740. The vitellogenin of B. germanica shows a notable similarity (between 32 and 42%) to those described in other insects, and its alignment shows a high number of motifs conserved in all species, especially in the subdomains I-V. Non-parsimony methods (Neighbor Joining) of phylogenetic analysis of the insect vitellogenin sequences gave a tree showing a topology that is, in general, congruent with the currently accepted insect phylogenetic schemes. Arch.
authors.affiliation: Department of Physiology and Molecular Biodiversity, Institut de Biologia Molecular de Barcelona (CID, CSIC), Barcelona, Spain.
authors.forename: D
authors.initials: D
authors.lastname: Comas
authors.forename: M D
authors.initials: MD
authors.lastname: Piulachs
authors.forename: X
authors.initials: X
authors.lastname: Bellés
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D020048:Blattellidae / Q000235:genetics*; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014819:Vitellogenins / Q000737:chemistry / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D014819:Vitellogenins