title: Single strand conformation polymorphism analysis of K-ras gene mutations by capillary electrophoresis with laser-induced fluorescence (LIF) detector.
abstract: Mutations of K-ras gene play an important role in neoplastic progression. The capillary electrophoresis-single strand conformation polymorphism (CE-SSCP) technique is available for the detection of gene mutations. Using an automated capillary electrophoresis with short-chain linear polyacrylamide, after denaturation of PCR products, injections were performed at reverse polarity of 5 kV for 15 s and the separations were carried out under a constant voltage of 8 kV. Of 16 specimens of lung cancer tissue, two specimens were found to have abnormal peaks in the electrophoretogram. CE-SSCP is rapid, automated, and has high performance.
authors.affiliation: Department of Pharmacy, Henan Medical University, Zhengzhou, 450052, China. zzzhyd@371.net
authors.forename: Z
authors.initials: Z
authors.lastname: Zhang
authors.forename: Y
authors.initials: Y
authors.lastname: Wu
authors.forename: W
authors.initials: W
authors.lastname: Cheng
authors.forename: R
authors.initials: R
authors.lastname: Wu
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D011905:Genes, ras; D006801:Humans; D007834:Lasers; D008175:Lung Neoplasms / Q000235:genetics*; D009154:Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D013050:Spectrometry, Fluorescence / Q000379:methods*