Macrophages synthesize factor X and secrete factor X/Xa-containing prothrombinase activity into the surrounding medium.
Activation of the coagulation cascade, mediated by various monocyte/macrophage procoagulants, is an important component in the pathology of inflammatory disease. The type of procoagulant expressed may vary between different monocyte/macrophage subtypes and may differ depending on how the cells are treated. In the present study we show that both murine peritoneal macrophages and human adherent synovial cells from rheumatoid arthritis lesions express prothrombinase activity that was inhibited by anti-Factor X antibodies. Northern blot analysis showed that Factor X was transcribed by the murine peritoneal cells and Western blot analysis showed the presence of Factor X antigen. Further experiments showed that the prothrombinase activity was secreted by the cells into the medium in a detergent-sensitive form, suggesting that the prothrombinase is released on small lipid-containing vesicles.
Pejler
G
G
Swedish University of Agricultural Sciences, Department of Veterinary Medical Chemistry, The Biomedical Center, Uppsala. Gunnar.Pejler@vmk.slu.se
Lunderius
C
C
Tomasini-Johansson
B
B
D000818:Animals; D004492:Edetic Acid / Q000494:pharmacology; D005170:Factor X / Q000096:biosynthesis* / Q000378:metabolism* / Q000494:pharmacology; D015951:Factor Xa / Q000378:metabolism* / Q000494:pharmacology; D005260:Female; D006801:Humans; D007700:Kinetics; D008264:Macrophages / Q000378:metabolism*; D017737:Macrophages, Peritoneal / Q000378:metabolism; D008297:Male; D051379:Mice; D017830:Octoxynol / Q000494:pharmacology; D011516:Prothrombin / Q000378:metabolism; D013582:Synovial Fluid / Q000166:cytology; D013925:Thromboplastin / Q000187:drug effects / Q000378:metabolism*
D011516:Prothrombin; D005170:Factor X; D017830:Octoxynol; D013925:Thromboplastin; D004492:Edetic Acid; D015951:Factor Xa