title: Targeting the chromatin-remodeling MSL complex of Drosophila to its sites of action on the X chromosome requires both acetyl transferase and ATPase activities.
abstract: Dosage compensation in Drosophila is mediated by a multiprotein, RNA-containing complex that associates with the X chromosome at multiple sites. We have investigated the role that the enzymatic activities of two complex components, the histone acetyltransferase activity of MOF and the ATPase activity of MLE, may have in the targeting and association of the complex with the X chromosome. Here we report that MLE and MOF activities are necessary for complexes to access the various X chromosome sites. The role that histone H4 acetylation plays in this process is supported by our observations that MOF overexpression leads to the ectopic association of the complex with autosomal sites.
authors.affiliation: Department of Biology, Emory University, Atlanta, GA 30322, USA.
authors.forename: W
authors.initials: W
authors.lastname: Gu
authors.forename: X
authors.initials: X
authors.lastname: Wei
authors.forename: A
authors.initials: A
authors.lastname: Pannuti
authors.forename: J C
authors.initials: JC
authors.lastname: Lucchesi
mesh_terms: D000107:Acetylation; D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D002843:Chromatin / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone; D004265:DNA Helicases; D004268:DNA-Binding Proteins; D004330:Drosophila / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins; D005260:Female; D005455:Fluorescent Antibody Technique; D051548:Histone Acetyltransferases; D006657:Histones / Q000378:metabolism; D017403:In Situ Hybridization; D009687:Nuclear Proteins / Q000378:metabolism*; D020365:RNA Helicases / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014960:X Chromosome
chemical_list: D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D006657:Histones; D009687:Nuclear Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C070837:mle protein, Drosophila; C082132:msl-1 protein, Drosophila; C094166:msl-2 protein, Drosophila; D000123:Acetyltransferases; D051548:Histone Acetyltransferases; D000251:Adenosine Triphosphatases; D004265:DNA Helicases; D020365:RNA Helicases