title: Inhibitory effects of morphine on some inflammation-related parameters in the goldfish Carassius auratus L.
abstract: Acute peritonitis induced in the goldfish by intraperitoneal injection of a sterile Thioglycollate solution shows a typical pattern with intraperitoneal exudation of serum proteins followed by influx of leucocytes (mainly heterophils/macrophages) correlated with elevated levels of chemotactic factors in peritoneal fluid and blood plasma. Supplementation of Thioglycollate with morphine (20 mg kg(-1) b.w.) does not affect the leakage of serum proteins into peritoneum. In contrast, it reduces the number of exudate peritoneal leucocytes (among them heterophils/macrophages) to the control level and decreases the level of peritoneal fluid/plasma chemoattractants, both effects being reversed by naltrexone pretreatment. Morphine itself acts as a chemokinetic factor for fish leucocytes as it increases their random movements. Therefore inhibitory effects of morphine on accumulation of exudate cells might be explained by inhibition of the production/release of chemotactic factors and/or reduced sensitivity of leucocytes to chemotactic signals. The effects of morphine on the goldfish peritonitis are in concordance with those described recently in Atlantic salmon and CB6 mice.
authors.affiliation: Department of Evolutionary Immunobiology, Institute of Zoology, Jagiellonian University, Krakow, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Chadzinska
authors.forename: A
authors.initials: A
authors.lastname: Scislowska-Czarnecka
authors.forename: B
authors.initials: B
authors.lastname: Plytycz
mesh_terms: D000701:Analgesics, Opioid / Q000008:administration & dosage / Q000276:immunology / Q000627:therapeutic use*; D000818:Animals; D002633:Chemotaxis; D004396:Coloring Agents / Q000737:chemistry; D005070:Evans Blue / Q000737:chemistry; D005122:Exudates and Transudates; D005393:Fish Diseases / Q000188:drug therapy / Q000276:immunology*; D006054:Goldfish / Q000276:immunology*; D007274:Injections, Intraperitoneal / Q000662:veterinary; D007958:Leukocyte Count / Q000662:veterinary; D009020:Morphine / Q000008:administration & dosage / Q000276:immunology / Q000627:therapeutic use*; D009271:Naltrexone / Q000008:administration & dosage; D009292:Narcotic Antagonists / Q000008:administration & dosage; D010538:Peritonitis / Q000188:drug therapy / Q000276:immunology / Q000662:veterinary*; D013864:Thioglycolates / Q000008:administration & dosage
chemical_list: D000701:Analgesics, Opioid; D004396:Coloring Agents; D009292:Narcotic Antagonists; D013864:Thioglycolates; D005070:Evans Blue; D009271:Naltrexone; D009020:Morphine