title: Structure and stress-related expression of two cDNAs encoding proteinase inhibitor II of Nicotiana glutinosa L.
abstract: Two cDNAs, pNGPI-1 and pNGPI-2, encoding Nicotiana glutinosa proteinase inhibitor II (PI-II) have been cloned, sequenced and identified. The deduced amino acid sequences are 54-82% identical to those of other plant PI-II. The NGPI-1 protein is composed of eight repeated domains, while NGPI-2 contains six repeated regions, each with a putative reactive site. The expression of NGPI-1 is highly regulated in a developmental- and tissue-specific manner, with the transcript being detected in young leaves and floral organs of N. glutinosa plants. In mature leaves, the NGPI-1 gene is rapidly activated by distinct temporal induction patterns in response to pathogen-related (biotic) and wound-related (abiotic) stresses.
authors.affiliation: Plant Cell Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology, Taejeon, South Korea.
authors.forename: D
authors.initials: D
authors.lastname: Choi
authors.forename: J A
authors.initials: JA
authors.lastname: Park
authors.forename: Y S
authors.initials: YS
authors.lastname: Seo
authors.forename: Y J
authors.initials: YJ
authors.lastname: Chun
authors.forename: W T
authors.initials: WT
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D018076:DNA, Complementary / Q000032:analysis; D018744:DNA, Plant / Q000032:analysis; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D010947:Plants, Toxic; D011480:Protease Inhibitors; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000235:genetics* / Q000821:virology; D014027:Tobacco Mosaic Virus
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D010940:Plant Proteins; D011480:Protease Inhibitors; C034104:proteinase inhibitor II protein, plant