The use of recombinant antibodies in proteomics.
Recombinant antibodies are becoming increasingly important in the field of proteomics. Recent advances include the development of large phage-antibody libraries that contain high-affinity binders to almost any target protein, and new methods for high-throughput selection of antibody-antigen interactions. Coupled with a range of new screening technologies that use high-density antibody arrays to identify differentially expressed proteins, these antibody libraries can be applied to whole proteome analysis.
Holt
L J
LJ
MRC Laboratory of Molecular Biology and MRC Centre for Protein Engineering, Hills Road, CB2 2QH, Cambridge, UK.
Enever
C
C
de Wildt
R M
RM
Tomlinson
I M
IM
D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology*; D020869:Gene Expression Profiling / Q000379:methods*; D005822:Genetic Vectors; D020543:Proteome / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D020543:Proteome; D011994:Recombinant Proteins