Elevated zinc induces siderophore biosynthesis genes and a zntA-like gene in Pseudomonas fluorescens.
Zinc-regulated genes were analyzed in Pseudomonas fluorescens employing mutagenesis with a reporter gene transposon. Six mutants responded with increased gene expression to elevated concentrations of zinc. Genetic and biochemical analysis revealed that in four of the six mutants the transposon had inserted into genes essential for the biosynthesis of the siderophore pyoverdine. The growth of one of the mutants was severely impaired in the presence of elevated concentrations of cadmium and zinc ions. In this mutant, the transposon had inserted in a gene with high similarity to P-type ATPases involved in zinc and cadmium ion transport. Four mutants reacted with reduced gene expression to elevated concentrations of zinc. One of these mutants was sensitive to zinc, cadmium and copper ions. The genetic region targeted in this mutant did not show similarity to any known gene.
Rossbach
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Department of Biological Sciences, Western Michigan University, 1201 Oliver Street, Kalamazoo, MI 49008, USA. silvia.rossbach@wmich.edu
Wilson
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Kukuk
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Carty
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HA
D000251:Adenosine Triphosphatases / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D004251:DNA Transposable Elements; D015964:Gene Expression Regulation, Bacterial; D019216:Metals, Heavy / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009154:Mutation; D011551:Pseudomonas fluorescens / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D017262:Siderophores / Q000096:biosynthesis* / Q000235:genetics; D015032:Zinc / Q000378:metabolism / Q000494:pharmacology*; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D004251:DNA Transposable Elements; D019216:Metals, Heavy; D017262:Siderophores; D001616:beta-Galactosidase; D000251:Adenosine Triphosphatases; C109898:Zn(II)-translocating P-type ATPase; D015032:Zinc