Antioxidants with carcinostatic activity (resveratrol, vitamin E and selenium) in modulation of blood platelet adhesion.
Compounds with potential antiplatelet activity can be used in the therapy of cardiovascular disorders. We investigated the effects of three different antioxidants with carcinostatic property: trans-resveratrol, Trolox a water-soluble analog of vitamin E, and inorganic selenocompounds (sodium selenite and selenate) on blood platelet adhesion to fibrinogen (Fg). Adhesion, the initial step of platelet activation, was estimated by the colorimetric method with BCA (bicinchoninic acid) solution in 96-well Fg-coated microtiter dishes. It was shown that resveratrol significantly inhibited adhesion of both thrombin- and ADP-activated platelets to Fg. After incubation of platelets for 30 min. at 37 degrees C with resveratrol at the concentration of 100 microg/ml above 40% inhibition of adhesion was achieved. The inhibition of platelet adhesion of Fg caused by Trolox was lower than by resveratrol and at higher concentration (1 mM) reached maximum 12%. We also demonstrated that neither sodium selenite nor selenate significantly altered platelet adhesion to Fg. We conclude that changed adhesion of blood platelets to Fg in the presence of resveratrol and Trolox, but not selenium may be the result of different antioxidative activities of tested compounds.
Zbikowska
H M
HM
Department of General Biochemistry, Institute of Biochemistry, University of Lódź, Poland.
Olas
B
B
D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects* / Q000502:physiology; D002839:Chromans / Q000494:pharmacology*; D010973:Platelet Adhesiveness / Q000187:drug effects* / Q000502:physiology; D000077185:Resveratrol; D064586:Selenic Acid; D018036:Selenium Compounds / Q000494:pharmacology; D018038:Sodium Selenite / Q000494:pharmacology; D013267:Stilbenes / Q000494:pharmacology*; D013552:Swine; D014810:Vitamin E / Q000031:analogs & derivatives
D016588:Anticarcinogenic Agents; D000975:Antioxidants; D002839:Chromans; D018036:Selenium Compounds; D013267:Stilbenes; D014810:Vitamin E; D018038:Sodium Selenite; D064586:Selenic Acid; D000077185:Resveratrol; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid