Comparative antioxidant potential of anaesthetics and perioperative drugs in vitro.
We have investigated the comparative antioxidant capacity of a range of anaesthetics (inhaled and intravenous) and perioperative neurosurgical drugs (at clinically relevant concentrations) using different radical species and assay methods in vitro. The highest levels of antioxidant activity against the ABTS(.+) radical were obtained with propofol (100 mmol/LTE) and dopamine (1080 mmol/LTE), respectively. However, only dopamine (12 mmol/l) showed antioxidant activity in protecting proteins in normal brain tissue from oxidative damage (assessed via SDS-PAGE analysis) induced by OH(.) or O(2)(-.) generated radiolytically in vitro. Neither dopamine nor propofol showed antioxidant activity against O(2)(-.) generated chemically via reaction between xanthine and xanthine oxidase in vitro. From these data, together with data on the relative antioxidant properties of anaesthetics/drugs obtained by other research groups which we have reviewed, we conclude that the apparent antioxidant activity of a given compound may depend entirely on the free radical species and/or the method of generation or assay employed. Finally, we suggest that on the basis of data obtained showing protection of brain proteins from oxidative damage induced by OH(.), or O(2)(-.) in vitro, further investigation into the in vivo antioxidant therapeutic potential of dopamine (or its analogues) on neurosurgical patients may be warranted.
Mantle
D
D
Department of Neurochemistry, Regional Neurosciences Centre, Newcastle General Hospital, New Castle-upon-Tyne NE4 6BE, UK.
Eddeb
F
F
Areni
K
K
Snowden
C
C
Mendelow
A D
AD
D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D018686:Anesthetics, Intravenous / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D005609:Free Radicals; D007430:Intraoperative Care
D018685:Anesthetics, Inhalation; D018686:Anesthetics, Intravenous; D000975:Antioxidants; D005609:Free Radicals