The use of transgenic yeasts expressing a gene encoding a glycosyl-hydrolase as a tool to increase resveratrol content in wine.
Resveratrol, a phenolic compound produced in grapes, exhibits properties that may contribute to the reduction of the incidence of coronary heart disease and other human health related processes. Recombinant yeast strains expressing the Aspergillus niger abfB gene encoding an alpha-L-arabinofuranosidase or the Candida molischiana bgIN gene encoding a beta-glucosidase have been used in vinifications as tools to increase the resveratrol content of white wine. Glycosylated resveratrol isomers (trans- and cis-piceid) and free resveratrol isomers (trans- and cis-resveratrol) were detected and quantified in white wines. Wines fermented with the strain expressing BgiN showed an increased amount of total resveratrol derivatives, particularly the non-glycosylated forms.
González-Candelas
L
L
Departamento de Ciencia de los Alimentos, Consejo Superior de Investigaciones Cientificas, Buriassot, Valencia, Spain.
Gil
J V
JV
Lamuela-Raventós
R M
RM
Ramón
D
D
D001234:Aspergillus niger / Q000235:genetics; D002175:Candida / Q000235:genetics; D006026:Glycoside Hydrolases / Q000235:genetics*; D000077185:Resveratrol; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013267:Stilbenes / Q000032:analysis*; D019076:Transgenes; D014920:Wine / Q000032:analysis*; D001617:beta-Glucosidase / Q000235:genetics*
D013267:Stilbenes; D006026:Glycoside Hydrolases; D001617:beta-Glucosidase; C019713:alpha-N-arabinofuranosidase; D000077185:Resveratrol