title: Effects of bromocriptine administration during the follicular phase of the oestrous cycle on prolactin and gonadotrophin secretion and follicular dynamics in merino monovular ewes.
abstract: Two experiments using Spanish Merino ewes were conducted to investigate whether the secretion of prolactin during the follicular phase of the sheep oestrous cycle was involved in the patterns of growth and regression of follicle populations. In both experiments, oestrus was synchronized with two cloprostenol injections which were administered 10 days apart. Concurrent with the second injection (time 0), ewes (n = 6 per group) received one of the following treatments every 12 h from time 0 to 72 h: group 1: vehicle injection (control); group 2: 0.6 mg bromocriptine (0.03 mg per kg per day); and group 3: 1.2 mg bromocriptine (0.06 mg per kg per day). In Expt 1, blood samples were collected every 3 h from 0 to 72 h, and also every 20 min from 38 to 54 h to measure prolactin, LH and FSH concentrations. In Expt 2, transrectal ultrasonography was carried out every 12 h from time 0 until oestrus, and blood samples were collected every 4 h to measure prolactin, LH and FSH concentrations. Ovulation rates were determined by laparoscopy on day 4 after oestrus. Bromocriptine markedly decreased prolactin secretion, but did not affect FSH concentrations, the mean time of the LH preovulatory surge or LH concentrations in the preovulatory surge. Both doses of bromocriptine caused a similar decrease in LH pulse frequency before the preovulatory surge. The highest bromocriptine dose led to a reduction (P < 0.01) in the number of 2-3 mm follicles detected in the ovaries at each time point. However, bromocriptine did not modify the total number or the number of newly detected 4-5 mm follicles at each time point, the number of follicles > 5 mm or the ovulation rate. In conclusion, the effects of bromocriptine on gonadotrophin and prolactin secretion and on the follicular dynamics during the follicular phase of the sheep oestrous cycle indicate that prolactin may influence the viability of gonadotrophin-responsive follicles shortly after luteolysis.
authors.affiliation: Departmento de Reproducción Animal, INIA, Avda. Puerta de Hierro Km 5,9. 28040-Madrid, Spain.
authors.forename: R A
authors.initials: RA
authors.lastname: Picazo
authors.forename: A
authors.initials: A
authors.lastname: González De Bulnes
authors.forename: A
authors.initials: A
authors.lastname: Gómez Brunet
authors.forename: A
authors.initials: A
authors.lastname: del Campo
authors.forename: B
authors.initials: B
authors.lastname: Granados
authors.forename: J
authors.initials: J
authors.lastname: Tresguerres
authors.forename: A
authors.initials: A
authors.lastname: López Sebastián
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001971:Bromocriptine / Q000494:pharmacology*; D004973:Estrus Synchronization; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D005498:Follicular Phase; D006727:Hormone Antagonists / Q000494:pharmacology*; D007986:Luteinizing Hormone / Q000378:metabolism; D006080:Ovarian Follicle / Q000187:drug effects*; D011388:Prolactin / Q000037:antagonists & inhibitors / Q000378:metabolism*; D012756:Sheep / Q000502:physiology*
chemical_list: D006727:Hormone Antagonists; D001971:Bromocriptine; D011388:Prolactin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone