Salmonella typhimurium as a basis for a live oral Echinococcus granulosus vaccine.
A live attenuated Salmonella typhimurium vaccine candidate, LVR01, was constructed by introducing a null deletion into the aroC gene of the parental canine S. typhimurium isolate, P228067. LVR01 was used to orally deliver to the canine immune system a fatty acid binding protein (FABP) from Echinococcus granulosus (EgDf1), as a fusion protein with fragment C (TetC) of tetanus toxin. Immunization studies demonstrated that live LVR01 is well tolerated by orally vaccinated dogs. There was no detectable shedding of the vaccine strain in the faeces 2 days after immunization. Humoral antibody responses were observed against Salmonella, TetC and EgDf1. Cellular responses were consistently detected against Salmonella and TetC. A cellular response against EgDf1 was also seen in a proportion of the LVR01 vaccinated dogs. We propose S. typhimurium LVR01 as a carrier for recombinant antigens and a vector for the construction of multivalent oral vaccines for dogs.
Chabalgoity
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Laboratory for Vaccine Research, Department of Biotechnology, Institute of Hygiene, Avda. A. Navarro 3051, Montevideo, CP 11600, Uruguay. crtn@bilbo.edu.uy
Moreno
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Carol
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Dougan
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Hormaeche
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D000284:Administration, Oral; D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis; D000947:Antigens, Helminth / Q000235:genetics; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004283:Dog Diseases / Q000276:immunology / Q000517:prevention & control; D004285:Dogs; D004443:Echinococcosis / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary; D004446:Echinococcus / Q000235:genetics* / Q000276:immunology*; D005798:Genes, Bacterial; D007074:Immunoglobulin G / Q000096:biosynthesis; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D012486:Salmonella typhimurium / Q000235:genetics* / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D017931:DNA Primers; D007074:Immunoglobulin G; D014614:Vaccines, Synthetic