Expression of VCAM-1, ICAM-1, E-selectin, and P-selectin on endothelium in situ in patients with erythroderma, mycosis fungoides and atopic dermatitis.
BACKGROUND
Erythroderma may result from different causes. At present it is unclear whether the patho-mechanisms that lead to these different types of erythroderma are identical or different. Adhesion molecules and their ligands play a major role in endothelial-leukocyte interactions, which affect the binding, transmigration and infiltration of lymphocytes and mononuclear cells during inflammation, injury, or immunological stimulation. The aim of this study was to investigate the adhesion molecule expression on endothelial cells in erythroderma in situ.


METHODS
Snap-frozen skin biopsy specimens from 23 patients with erythroderma were studied. Eight had idiopathic erythroderma, 5 erythrodermic atopic dermatitis, 4 Sézary syndrome and 6 had erythroderma from miscellaneous causes. As a control we studied skin specimens from 10 patients with mycosis fungoides, 5 patients with atopic dermatitis and 5 healthy non-atopic volunteers. To determine adhesion molecule expression on endothelial cells in situ, sections were immuno-histochemically double stained with biotinylated Ulex Europaeus agglutinin 1 as a pan-endothelial cell marker, and for the adhesion molecules VCAM-1, ICAM-1, E-, and P-selectin. All double- and single-stained blood vessels in the dermis were counted.


RESULTS
Mean endothelial expression in erythroderma was as follows: VCAM-1 51.4%, ICAM-1 70.1%, E-selectin 43.5%, and P-selectin 52.6%. There was no statistical difference between different groups of erythroderma. Mean expression of all adhesion molecules tested, was in Sézary syndrome higher than in mycosis fungoides albeit not significant. In erythrodermic atopic dermatitis only VCAM-1 expression was significantly higher than in lesional skin of atopic dermatitis. No differences were observed in expression of the other three adhesion molecules.


CONCLUSIONS
There is no difference regarding adhesion molecule expression on endothelial cells between different types of erythroderma.
Sigurdsson
V
V
Department of Dermatology and Allergology, University Medical Center Utrecht, The Netherlands. V.Sigurdsson@azu.nl
de Vries
I J
IJ
Toonstra
J
J
Bihari
I C
IC
Thepen
T
T
Bruijnzeel-Koomen
C A
CA
van Vloten
W A
WA
D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D003876:Dermatitis, Atopic / Q000378:metabolism* / Q000473:pathology; D003873:Dermatitis, Exfoliative / Q000378:metabolism* / Q000473:pathology; D019040:E-Selectin / Q000096:biosynthesis; D004727:Endothelium / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis; D009182:Mycosis Fungoides / Q000378:metabolism* / Q000473:pathology; D019007:P-Selectin / Q000096:biosynthesis; D012878:Skin Neoplasms / Q000378:metabolism* / Q000473:pathology; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis
D015815:Cell Adhesion Molecules; D019040:E-Selectin; D019007:P-Selectin; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1