Inhibition of N-linked glycosylation causes apoptosis in hamster BHK21 cells.
The tsBN7 cell line is one of the temperature-sensitive mutants for cell proliferation derived from hamster BHK21 cell line. It has a mutation in the DAD1 gene and enters apoptosis at the restrictive temperature of 39 degrees C. The defect of Dad1p causes a loss of N-linked glycosylation; therefore, it was thought that an inhibition of N-linked glycosylation induced apoptosis.However, tunicamycin, a potent inhibitor of N-linked glycosylation, had not caused apoptosis in wild-type BHK21 cells. In order to clarify this discrepancy, wild-type BHK21 cells treated with tunicamycin and tsBN7 cells incubated at 39.5 degrees C were examined by the annexin V staining and TUNEL methods. Both methods showed that tunicamycin induces apoptosis in wild-type BHK21 cells, similar to the defect of Dad1p. Thus, we concluded that loss of N-linked glycosylation causes apoptosis.
Yoshimi
M
M
Kyushu University, Department of Molecular Biology, Fukuoka, 812-8582, Japan.
Sekiguchi
T
T
Hara
N
N
Nishimoto
T
T
D000818:Animals; D017304:Annexin A5 / Q000032:analysis; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D006224:Cricetinae; D005434:Flow Cytometry; D006031:Glycosylation / Q000187:drug effects; D020287:In Situ Nick-End Labeling; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D013696:Temperature; D014415:Tunicamycin / Q000494:pharmacology*
D017304:Annexin A5; D008565:Membrane Proteins; D014415:Tunicamycin