[Detection of tubercle bacilli in clinical samples using genetic methods(PCR)compared with Lowenstein-Jensen culture methods].
The aim of this work was to compare data obtained using PCR assay for detection of Mycobacterium tuberculosis with culture. We report a test for detection of tubercle bacilli by PCR and identification at species level by capture plate hybridisation and enzyme-linked immunoassay. 222 clinical samples obtained from patients with confirmed and suspected tuberculosis were analysed. These specimens were tested parallelly by conventional culture on Löwenstein-Jensen slants and PCR test. For 205 samples a complete agreement between these methods was observed.
Boruń
M
M
Centrum Mikrobiologii i Wirusologii, Polskiej Akademii Nauk w Lodzi.
Rybczyńska
M
M
Pawłowska
I
I
D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006824:Hybridization, Genetic; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D013045:Species Specificity; D014397:Tuberculosis, Pulmonary / Q000382:microbiology*