title: Growth of Photorhabdus luminescens in batch and glucose fed-batch culture.
abstract: Photorhabdus luminescens, a bacterial symbiont of entomopathogenic biocontrol nematodes, was grown in batch and glucose fed-batch culture. The cell density, bioluminescence, production of antibiotic substances, number of cells with inclusion bodies, glucose concentration and oxygen uptake rate were recorded. The addition of 12.4 g 1(-1) glucose prolonged the growth, and the yield almost doubled, from 6.85 g 1(-1) to 12.45 g 1(-1) dry mass. The production of antibiotic substances increased by 140%. Bioluminescence was higher in the batch culture. A shift of P. luminescens to phase II variants was not detected.
authors.affiliation: Institute for Phytopathology, Department of Biotechnology and Biological Control, Christian-Albrechts-University Kiel, Raisdorf, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Jeffke
authors.forename: D
authors.initials: D
authors.lastname: Jende
authors.forename: C
authors.initials: C
authors.lastname: Mätje
authors.forename: R U
authors.initials: RU
authors.lastname: Ehlers
authors.forename: L
authors.initials: L
authors.lastname: Berthe-Corti
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D019149:Bioreactors; D015169:Colony Count, Microbial; D003470:Culture Media; D005947:Glucose / Q000378:metabolism*; D002479:Inclusion Bodies / Q000378:metabolism; D008163:Luminescent Measurements; D010101:Oxygen Consumption; D020637:Photorhabdus / Q000254:growth & development* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D005947:Glucose