Dispersion of the Cf-4 disease resistance gene in Lycopersicon germplasm.
In the past, numerous Cf genes have been reported in tomato (Lycopersicon esculentum Mill.) that confer resistance against leaf mould (Cladosporium fulvum Cke.). We are interested in genetic variation at Cf loci. Therefore, previously uncharacterized Cf genes were further analysed. Recognition of the AVR4 elicitor, DNA gel blot analysis, PCR analysis and sequencing of part of the Cf-4 locus showed that a large proportion of the accessions tested harboured the Cf-4 resistance gene. We concluded that despite differences in nomenclature, all these accessions harbour the same Cf-4 locus, probably introgressed from the same donor. The origin of the Cf-4 locus and the reasons for discrepancies with earlier reports are discussed.
Haanstra
J P
JP
The Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, Department of Plant Breeding, PO Box 386, 6700 AJ Wageningen, The Netherlands.
Thomas
C M
CM
Jones
J D
JD
Lindhout
P
P
D015139:Blotting, Southern; D005838:Genotype; D018551:Solanum lycopersicum / Q000235:genetics; D008562:Membrane Glycoproteins / Q000235:genetics*; D008957:Models, Genetic; D010935:Plant Diseases / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
C109609:Cf protein, Lycopersicon esculentum; D008562:Membrane Glycoproteins; D010940:Plant Proteins