title: Detection of genes essential in specific niches by signature-tagged mutagenesis.
abstract: Variations of the signature-tagged mutagenesis (STM) technique are now possible and the method can be applied to most pathogens that have an STM-selectable phenotype in a host system. STM screening of 15,040 mutants from 11 bacterial species identified 323 in vivo attenuated mutants. As a genome-scanning tool, STM will yield information about genes with unknown functions as well as information crucial for understanding microbial pathogenesis.
authors.affiliation: Microbiologie Moléculaire et Génie des Protéines, Pavillon Charles-Eugène Marchand et Faculté de Médecine, Université Laval, Sainte-Foy, G1K 7P4, Québec, Canada.
authors.forename: D E
authors.initials: DE
authors.lastname: Lehoux
authors.forename: R C
authors.initials: RC
authors.lastname: Levesque
mesh_terms: D000818:Animals; D001419:Bacteria / Q000472:pathogenicity; D005658:Fungi / Q000472:pathogenicity; D005821:Genetic Techniques; D016254:Mutagenesis, Insertional / Q000379:methods*; D010271:Parasites / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics*