title: The sensitivity and specificity of a reverse transcription-polymerase chain reaction assay for the avian pneumovirus (Colorado strain).
abstract: A reverse transcription-polymerase chain reaction (RT-PCR) assay for the detection of avian pneumovirus (APV), Colorado strain (US/CO), was evaluated for sensitivity and specificity. The single-tube RT-PCR assay utilized primers developed from the matrix (M) gene sequence of the US/CO APV. The RT-PCR amplified the US/CO APV but did not amplify other pneumoviruses, including the avian pneumoviruses subgroups A and B. The RT-PCR was capable of detecting between 10(0.25) mean tissue culture infective dose (TCID50) and 10(-0.44) TCID50 of the US/CO APV. These results have demonstrated that the single-tube RT-PCR assay is a specific and sensitive assay for the detection of US/CO APV.
authors.affiliation: National Veterinary Services Laboratories, Veterinary Services, Animal and Plant Health Inspection Service, U.S. Department of Agriculture, Ames, IA 50010, USA.
authors.forename: J C
authors.initials: JC
authors.lastname: Pedersen
authors.forename: D L
authors.initials: DL
authors.lastname: Reynolds
authors.forename: A
authors.initials: A
authors.lastname: Ali
mesh_terms: D000818:Animals; D002642:Chick Embryo; D003120:Colorado; D018111:Pneumovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D018186:Pneumovirus Infections / Q000175:diagnosis / Q000662:veterinary; D011201:Poultry Diseases / Q000175:diagnosis / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary*; D012680:Sensitivity and Specificity; D014422:Turkeys