title: Transactivation of latent Marek's disease herpesvirus genes in QT35, a quail fibroblast cell line, by herpesvirus of turkeys.
abstract: The QT35 cell line was established from a methylcholanthrene-induced tumor in Japanese quail (Coturnix coturnix japonica) (C. Moscovici, M. G. Moscovici, H. Jimenez, M. M. Lai, M. J. Hayman, and P. K. Vogt, Cell 11:95-103, 1977). Two independently maintained sublines of QT35 were found to be positive for Marek's disease virus (MDV)-like genes by Southern blotting and PCR assays. Sequence analysis of fragments of the ICP4, ICP22, ICP27, VP16, meq, pp14, pp38, open reading frame (ORF) L1, and glycoprotein B (gB) genes showed a strong homology with the corresponding fragments of MDV genes. Subsequently, a serotype 1 MDV-like herpesvirus, tentatively name QMDV, was rescued from QT35 cells in chicken kidney cell (CKC) cultures established from 6- to 9-day-old chicks inoculated at 8 days of embryonation with QT35 cells. Transmission electron microscopy failed to show herpesvirus particles in QT35 cells, but typical intranuclear herpesvirus particles were detected in CKCs. Reverse transcription-PCR analysis showed that the following QMDV transcripts were present in QT35 cells: sense and antisense meq, ORF L1, ICP4, and latency-associated transcripts, which are antisense to ICP4. A transcript of approximately 4.5 kb was detected by Northern blotting using total RNA from QT35 cells. Inoculation of QT35 cells with herpesvirus of turkeys (HVT)-infected chicken embryo fibroblasts (CEF) but not with uninfected CEF resulted in the activation of ICP22, ICP27, VP16, pp38, and gB. In addition, the level of ICP4 mRNA was increased compared to that in QT35 cells. The activation by HVT resulted in the production of pp38 protein. It was not possible to detect if the other activated genes were translated due to the lack of serotype 1-specific monoclonal antibodies.
authors.affiliation: Unit of Avian Health, Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA.
authors.forename: T
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: S L
authors.initials: SL
authors.lastname: Kaplan
authors.forename: P
authors.initials: P
authors.lastname: Wakenell
authors.forename: K A
authors.initials: KA
authors.lastname: Schat
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000378:metabolism; D015139:Blotting, Southern; D002478:Cells, Cultured; D003370:Coturnix; D005347:Fibroblasts / Q000821:virology*; D006564:Herpesviridae / Q000235:genetics* / Q000502:physiology; D008381:Herpesvirus 2, Gallid / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011784:Quail; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D015533:Transcriptional Activation; D014422:Turkeys / Q000821:virology; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D017735:Virus Latency
chemical_list: D000956:Antigens, Viral; D010750:Phosphoproteins; D014764:Viral Proteins; C071698:phosphoprotein pp38, Marek's disease virus