Molecular cloning of the human kallikrein 15 gene (KLK15). Up-regulation in prostate cancer.
Kallikreins are a subgroup of serine proteases with diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis. By using molecular cloning techniques, we identified a new human kallikrein gene, tentatively named KLK15 (for kallikrein 15 gene). This new gene maps to chromosome 19q13.4 and is located between the KLK1 and KLK3 genes. KLK15 is formed of five coding exons and four introns, and shows structural similarity to other kallikreins and kallikrein-like genes. KLK15 has three alternatively spliced forms and is primarily expressed in the thyroid gland and to a lower extent in the prostate, salivary, and adrenal glands and in the colon testis and kidney. Our preliminary results indicate that the expression of KLK15 is up-regulated by steroid hormones in the LNCaP prostate cancer cell line. The KLK15 gene is also up-regulated, at the mRNA level, in prostate cancer in comparison to normal prostatic tissue. KLK15 up-regulation was found to be associated with more aggressive forms of prostate cancer. This newly discovered gene has the potential of being used as a diagnostic and/or prognostic marker for prostate cancer.
Yousef
G M
GM
Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada.
Scorilas
A
A
Jung
K
K
Ashworth
L K
LK
Diamandis
E P
EP
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics; D003001:Cloning, Molecular; D013196:Dihydrotestosterone / Q000494:pharmacology; D005091:Exons / Q000235:genetics; D020869:Gene Expression Profiling; D006801:Humans; D007438:Introns / Q000235:genetics; D007610:Kallikreins / Q000737:chemistry / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000737:chemistry / Q000235:genetics*; D010802:Phylogeny; D020161:Physical Chromosome Mapping; D017430:Prostate-Specific Antigen / Q000737:chemistry; D011471:Prostatic Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D014407:Tumor Cells, Cultured; D015854:Up-Regulation / Q000187:drug effects
D009363:Neoplasm Proteins; D012333:RNA, Messenger; D013196:Dihydrotestosterone; C501608:KLK15 protein, human; D007610:Kallikreins; D017430:Prostate-Specific Antigen