Effect of subchronic bimoclomol treatment on vascular responsiveness and heat shock protein production in spontaneously hypertensive rats.
This study was designed to investigate the effects of subchronic bimoclomol treatment on endothelial function and expression of 72 kD heat shock protein (HSP-72) in spontaneously hypertensive rats. Endothelial function was tested by monitoring vascular responses to acetylcholine in isolated aortic ring preparations. Polymerase chain reaction was applied to determine HSP-72 mRNA content in the tissue samples. Significant age-dependent declines in relaxation to acetylcholine and vascular HSP-72 mRNA levels were observed in the spontaneously hypertensive animals. Both changes were prevented by subchronic application of bimoclomol suggesting that preservation of endothelial function might be related to sustained levels of HSP-72.
Jednákovits
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Biorex Research and Development Co., Veszprém, Hungary. andrea_jednakovits@rex.biorex.hu
Kurucz
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Nánási
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PP
D000109:Acetylcholine / Q000494:pharmacology; D000375:Aging / Q000378:metabolism / Q000502:physiology; D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology; D001013:Aorta, Thoracic / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D001794:Blood Pressure / Q000187:drug effects; D002216:Captopril / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D006973:Hypertension / Q000188:drug therapy / Q000378:metabolism / Q000503:physiopathology*; D007094:Imides / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D008729:Methoxamine / Q000494:pharmacology; D009126:Muscle Relaxation / Q000187:drug effects / Q000502:physiology; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D011725:Pyridines / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR
D000959:Antihypertensive Agents; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D007094:Imides; D011725:Pyridines; D012333:RNA, Messenger; D002216:Captopril; C108730:bimoclomol; D008729:Methoxamine; D000109:Acetylcholine