title: Binding of soluble glycoproteins from sugarcane juice to cells of Acetobacter diazotrophicus.
abstract: Sugarcane produces two different pools of glycoproteins containing a heterofructan as glycidic moiety, tentatively defined as high-molecular mass (HMMG) and mid-molecular mass (MMMG) glycoproteins. Both kinds of glycoproteins can be recovered in sugarcane juice. Fluorescein-labelled glycoproteins are able to bind to Acetobacter diazotrophicus cells, a natural endophyte of sugarcane. This property implies the aggregation of bacterial cells in liquid culture after addition of HMMG or MMMG. Anionic glycoproteins seem to be responsible for the binding activity whereas cationic fraction is not retained on the surface ofA. diazotrophicus. Bound HMMG is competitively desorbed by sucrose whereas MMMG is desorbed by glucosamine or fructose. On this basis, a hypothesis about the discriminatory ability of sugarcane to choose the compatible endophyte from several possible ones is proposed.
authors.affiliation: Department of Plant Physiology, Faculty of Biology, Complutense University of Madrid, Spain.
authors.forename: M E
authors.initials: ME
authors.lastname: Legaz
authors.forename: R
authors.initials: R
authors.lastname: de Armas
authors.forename: E
authors.initials: E
authors.lastname: Barriguete
authors.forename: C
authors.initials: C
authors.lastname: Vicente
mesh_terms: D000091:Acetobacter / Q000378:metabolism*; D001422:Bacterial Adhesion; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D005630:Fructans / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009586:Nitrogen Fixation; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D010945:Plants, Edible / Q000737:chemistry / Q000382:microbiology*; D011485:Protein Binding; D013045:Species Specificity
chemical_list: D005630:Fructans; D006023:Glycoproteins; D010940:Plant Proteins