title: Analysis of genotype frequencies and interlocus association for the PM, DQA1, and D1S80 loci in four populations.
abstract: Allele frequencies of the LDLR, HBGG, GYPA, D7S8, GC, DQA1, and D1S80 loci are presented and genotypes are analyzed for each of four ethnic groups: African Americans (n = 200), US Caucasians (n = 200), US Hispanics (n = 200), and Japanese (n = 89). Hardy-Weinberg genotypic proportions were observed in all but two of the 28 population-locus tests undertaken. Those two instances are attributable to type I statistical error. Gametic equilibrium among loci is an assumption invoked for application of the product rule to utilize the discriminatory power from two or more loci simultaneously. Two statistical methods, a genotype matching statistic and log-linear modeling, were used to evaluate gametic disequilibrium. The match statistic, comparing observed to expected likelihood of genotypic identity for seven loci among pairs of individuals within the database, revealed only one statistically significant deviation among 20 tests. As expected, the probability of match was generally lowest in the test on all ethnic groups combined, indicating that allele frequencies differ among ethnic groups for some of the loci. This was confirmed with the statistic theta to measure ethnic stratification, in which about 0.10 of the genetic variation is apportioned among the four ethnic groups for four of the structural loci (LDLR, HBGG, GC, and DQA1), while for GYPA, D7S8, and D1S80, variation is more uniformly distributed among ethnic groups. Log-linear modeling was also applied to the five PM loci. The most parsimonious log-linear model included only three higher order terms: the two-way interactions of three of the PM loci with ethnic group. These three instances (LDLR, HBGG, and GC) indicated differences in allele frequencies between ethnic groups. No two or higher way interaction (disequilibrium) was observed among loci. In summary, the assumptions of Hardy-Weinberg and gametic equilibrium that facilitate the use of the five PM loci, DQA1 and D1S80 in forensic applications are consistent with the allele and genotype frequencies observed in these populations.
authors.affiliation: School of Public Health, University of California, Berkeley 94720, USA.
authors.forename: W
authors.initials: W
authors.lastname: Klitz
authors.forename: R
authors.initials: R
authors.lastname: Reynolds
authors.forename: J
authors.initials: J
authors.lastname: Chen
authors.forename: H A
authors.initials: HA
authors.lastname: Erlich
mesh_terms: D044466:Asian People; D044383:Black People; D005554:Forensic Medicine; D005828:Genetics, Population; D005838:Genotype; D006680:HLA Antigens / Q000235:genetics*; D006630:Hispanic or Latino; D006801:Humans; D015233:Models, Statistical; D044465:White People; D001741:Black or African American
chemical_list: D006680:HLA Antigens