Trypsinogen expression in colorectal cancers.
The purpose of this study was to evaluate the trypsinogen expression in human colorectal cancers. We observed the trypsinogen-1 mRNA expression in five of ten human colorectal cancer cell lines by reverse transcriptase-polymerase chain reaction (RT-PCR), and gelatinolytic activity in conditioned medium of cancer cells at acidic pH by gelatin zymography. Furthermore, trypsinogen protein expression was observed in 68 out of 154 (44.2%) surgical specimens of colorectal cancer immunohistochemically. However, there was no statistically significant relationship between the trypsinogen expression and the clinicopathological findings. These findings suggest that trypsinogen might be involved in cancer invasion and metastasis throughout the cancer progression.
Oyama
K
K
Department of Surgery II, School of medicine, Kanazawa University, Kanazawa 920-8640, Japan.
Ohta
T
T
Nishimura
G I
GI
Elnemr
A
A
Yasui
T
T
Fujimura
T
T
Fushida
S
S
Kitagawa
H
H
Kayahara
M
M
Terada
T
T
Miwa
K
K
D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000473:pathology*; D017077:Culture Media, Conditioned / Q000378:metabolism; D005780:Gelatin / Q000378:metabolism; D019073:HT29 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007150:Immunohistochemistry; D009362:Neoplasm Metastasis; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014362:Trypsinogen / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D017077:Culture Media, Conditioned; D012333:RNA, Messenger; D005780:Gelatin; D014362:Trypsinogen