Effect of triclosan on interferon-gamma production and major histocompatibility complex class II expression in human gingival fibroblasts.
OBJECTIVE
The effect of triclosan (2,4,4'-trichloro-2'-hydroxyl-diphenyl ether) on the production of interferon-gamma (IFN-gamma) and the expression of major histocompatibility complex (MHC) class II antigen was studied in human gingival fibroblasts isolated from 4 individuals.


RESULTS
All cell lines demonstrated high IFN-gamma production in 24-h cultures of human gingival fibroblasts stimulated by phytohemagglutinin (PHA) (5 microg/ml). Human gingival fibroblasts showed a high expression of MHC class II when stimulated with 500 and 1,000 pg/ml rIFN-gamma in 7-day cultures. Treatment of the cells with triclosan (0.5 microg/ml) reduced both IFN-gamma production and MHC class II expression in human gingival fibroblast cultures. Similar inhibitory effects on IFN-gamma production and MHC class II expression were observed when the anti-inflammatory agent dexamethazone (1 microM) was used.


CONCLUSIONS
The present study further supports the view that the agent has an anti-inflammatory effect in addition to its antibacterial capacity.
Mustafa
M
M
Department of Pediatric Dentistry, Faculty of Odontology, Karolinska Institutet, Stockholm, Sweden. manal.mustafa@ofa.ki.se
Bakhiet
M
M
Wondimu
B
B
Modéer
T
T
D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D002478:Cells, Cultured; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005881:Gingiva / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis*; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D010835:Phytohemagglutinins / Q000494:pharmacology; D014260:Triclosan / Q000494:pharmacology*
D000891:Anti-Infective Agents, Local; D000949:Histocompatibility Antigens Class II; D010835:Phytohemagglutinins; D014260:Triclosan; D007371:Interferon-gamma