title: Analysis of tobramycin by liquid chromatography with pulsed electrochemical detection.
abstract: The determination of tobramycin by liquid chromatography using a column packed with poly(styrene-divinylbenzene) and pulsed electrochemical detection on a gold electrode is described. The mobile phase consisted of an aqueous solution containing 52 g/l of sodium sulfate, 1.5 g/l of sodium octanesulfonate and 50 ml/l of a 0.2 M phosphate buffer pH 3.0. The total time of analysis was not more than 30 min. The effects of the different chromatographic parameters on the separation were also investigated. When a number of commercial samples of tobramycin was analyzed using this method, nine different components were separated, five of which are of unknown identity.
authors.affiliation: Laboratorium voor Farmaceutische Chemie en Analyse van Geneesmiddelen, Faculteit Farmaceutische Wetenschappen, Katholieke Universiteit Leuven, Belgium.
authors.forename: J
authors.initials: J
authors.lastname: Szúnyog
authors.forename: E
authors.initials: E
authors.lastname: Adams
authors.forename: E
authors.initials: E
authors.lastname: Roets
authors.forename: J
authors.initials: J
authors.lastname: Hoogmartens
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis; D002853:Chromatography, Liquid; D004563:Electrochemistry; D007202:Indicators and Reagents; D011137:Polystyrenes; D012015:Reference Standards; D012996:Solutions; D014031:Tobramycin / Q000032:analysis*; D014753:Vinyl Compounds
chemical_list: D000900:Anti-Bacterial Agents; D007202:Indicators and Reagents; D011137:Polystyrenes; D012996:Solutions; D014753:Vinyl Compounds; C004985:divinyl benzene; D014031:Tobramycin