Founder's Award, Society for Biomaterials. Sixth World Biomaterials Congress 2000, Kamuela, HI,May 15-20, 2000. Really smart bioconjugates of smart polymers and receptor proteins.
Over the past 18 years we have been deeply involved with the synthesis and applications of stimuli-responsive polymer systems, especially polymer-biomolecule conjugates. This article summarizes our work with one of these conjugate systems, specifically polymer-protein conjugates. We include conjugates prepared by random polymer conjugation to lysine amino groups, and also those prepared by site-specific conjugation of the polymer to specific amino acid sites that are genetically engineered into the known amino acid sequence of the protein. We describe the preparation and properties of thermally sensitive random conjugates to enzymes and several affinity recognition proteins. We have also prepared site-specific conjugates to streptavidin with temperature-sensitive polymers, pH-sensitive polymers, and light-sensitive polymers. The preparation of these conjugates and their many fascinating applications are reviewed in this article.
Hoffman
A S
AS
University of Washington, Seattle, Washington, USA.
Stayton
P S
PS
Bulmus
V
V
Chen
G
G
Chen
J
J
Cheung
C
C
Chilkoti
A
A
Ding
Z
Z
Dong
L
L
Fong
R
R
Lackey
C A
CA
Long
C J
CJ
Miura
M
M
Morris
J E
JE
Murthy
N
N
Nabeshima
Y
Y
Park
T G
TG
Press
O W
OW
Shimoboji
T
T
Shoemaker
S
S
Yang
H J
HJ
Monji
N
N
Nowinski
R C
RC
Cole
C A
CA
Priest
J H
JH
Harris
J M
JM
Nakamae
K
K
Nishino
T
T
Miyata
T
T
D000178:Acrylamides / Q000737:chemistry*; D000180:Acrylic Resins; D019943:Amino Acid Substitution; D001363:Awards and Prizes; D001672:Biocompatible Materials / Q000737:chemistry* / Q000528:radiation effects; D001704:Biopolymers / Q000737:chemistry* / Q000528:radiation effects; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D020100:Hydrogels; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay / Q000379:methods; D008027:Light; D008422:Materials Testing; D015394:Molecular Structure; D016297:Mutagenesis, Site-Directed; D015202:Protein Engineering; D012958:Societies, Scientific; D012995:Solubility; D019809:Streptavidin / Q000031:analogs & derivatives* / Q000737:chemistry; D013696:Temperature
D000178:Acrylamides; D000180:Acrylic Resins; D001672:Biocompatible Materials; D001704:Biopolymers; D020100:Hydrogels; C119790:streptavidin-poly(N-isopropylacrylamide); D019809:Streptavidin