Lectins from bulbs of the Chinese daffodil Narcissus tazetta (family Amaryllidaceae).
The isolation of three lectins with similar N-terminal amino acid sequences from the bulbs of the Chinese daffodil Narcissus tazetta was achieved. The isolation protocol involved ion exchange chromatography on DEAE-cellulose, affinity chromatography on mannose-agarose, and fast protein liquid chromatography-gel filtration on Superose 12. The lectins were all adsorbed on mannose-agarose and demonstrated a single band with a molecular weight of 13 kDa in SDS-polyacrylamide gel electrophoresis and a single 26 kDa peak in gel filtration, indicating that they were mannose-binding, dimeric proteins. The lectins differed in hemagglutinating activity, with the magnitude of the activity correlating with the ionic strength of the buffer required to elute the lectin from the DEAE-cellulose column. The bulb lectin did not exert potent cytotoxicity against cancer cell lines or fetal bovine lung cells but inhibited syncytium formation in, and reinstated viability of, fetal bovine lung cells infected with bovine immunodeficiency virus.
Ooi
L S
LS
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, NT.
Ng
T B
TB
Geng
Y
Y
Ooi
V E
VE
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002845:Chromatography; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D004591:Electrophoresis, Polyacrylamide Gel; D015726:Giant Cells / Q000187:drug effects; D006386:Hemagglutination Tests; D006801:Humans; D016087:Immunodeficiency Virus, Bovine / Q000187:drug effects / Q000502:physiology; D037102:Lectins / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D008168:Lung; D019684:Magnoliopsida / Q000737:chemistry*; D051379:Mice; D008969:Molecular Sequence Data; D037121:Plant Lectins; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D016415:Sequence Alignment; D014407:Tumor Cells, Cultured
D037102:Lectins; D037121:Plant Lectins; D010940:Plant Proteins