title: Induction of mucosal and systemic antibody specific for SeMF3 of Streptococcus equi by intranasal vaccination using a sucrose acetate isobutyrate based delivery system.
abstract: Streptococcus equi causes equine strangles, a highly contagious disease of the upper respiratory tract. The antiphagocytic surface protein SeM is strongly immunogenic and evokes mucosal and systemic antibodies during convalescence. The present study investigated the potential of sucrose acetate isobutyrate (SAIB); a high viscosity excipient that provides controlled release of biologically active substances, to enhance antibody responses following intranasal immunization of horses with a 108 a.a. peptide of SeM (SeMF3). SeMF3-SAIB was administered intranasally to each of the 11 adult horses on days 0 and 28. A second group of seven horses was vaccinated with SeMF3 alone. SAIB enhanced the mucosal and systemic immunogenicity of SeMF3, whereas SeMF3 by itself stimulated only a shortlived mucosal IgA and no systemic response. Moreover, nasal mucosal responses of horses immunized with SeMF3-SAIB were qualitatively and quantitatively similar to those observed in convalescent horses and involved similar linear epitopes of SeM. Epitope analysis also suggested that the nasal response was different from that observed in serum. A booster response was obtained after the second vaccination. These results suggest that SAIB has potential as a vehicle for intranasal immunization of horses with antigenic peptides.
authors.affiliation: 108 Maxwell H. Gluck Equine Research Center, University of Kentucky, Lexington, KY 40546-0099, USA.
authors.forename: J E
authors.initials: JE
authors.lastname: Nally
authors.forename: S
authors.initials: S
authors.lastname: Artiushin
authors.forename: A S
authors.initials: AS
authors.lastname: Sheoran
authors.forename: P J
authors.initials: PJ
authors.lastname: Burns
authors.forename: B
authors.initials: B
authors.lastname: Simon
authors.forename: R M
authors.initials: RM
authors.lastname: Gilley
authors.forename: J
authors.initials: J
authors.lastname: Gibson
authors.forename: S
authors.initials: S
authors.lastname: Sullivan
authors.forename: J F
authors.initials: JF
authors.lastname: Timoney
mesh_terms: D000281:Administration, Intranasal; D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D000942:Antigens, Bacterial / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D001428:Bacterial Vaccines / Q000008:administration & dosage*; D016503:Drug Delivery Systems; D005260:Female; D006734:Horse Diseases / Q000517:prevention & control; D006736:Horses; D018928:Immunity, Mucosal; D007070:Immunoglobulin A / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000096:biosynthesis; D008969:Molecular Sequence Data; D013290:Streptococcal Infections / Q000517:prevention & control / Q000662:veterinary; D018502:Streptococcus equi / Q000276:immunology*; D013395:Sucrose / Q000031:analogs & derivatives; D014614:Vaccines, Synthetic / Q000008:administration & dosage
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D007070:Immunoglobulin A; D007074:Immunoglobulin G; C107891:SeM protein, Streptococcus equi; D014614:Vaccines, Synthetic; D013395:Sucrose; C002885:sucrose acetate isobutyrate