title: Effect of high-pressure treatment on lipoxygenase activity.
abstract: Solutions of commercial soybean lipoxygenase (100 microgram/ML in 0.2 M citrate-phosphate and 0.2 M Tris buffer were subjected to pressures of 0.1, 200, 400, and 600 MPa for 20 mm. The enzyme was stable at atmospheric pressure (0.1 MPa) over a wide pH range (5-9). In citrate phosphate buffer, the enzyme had maximum stability over the pH range 58 in untreated samples and after treatment at 200 MPa, but with increasing pressure, the pH stability range become narrower and centered around pH 78. The enzyme was more sensitive to acid than alkali, and at pH 9, it lost virtually all activity after pressurization at 600 MPa for 20 mm in both buffers. The activity of the crude enzyme extracted from tomatoes treated at 200 and 300 MPa for 10 mm was not significantly different from that of the untreated tomatoes, while a pressure of 400 MPa for 10 mm caused a significant decrease in activity and treatment at 600 MPa led to complete and irreversible activity loss. Compared to unpressurized tomatoes, treatment at 600 MPa gave significantly reduced levels of hexanal, cis-3-hexenal, and trans-2-hexenal, which are important contributors to "fresh" tomato flavor, and this was attributed to the inactivation of lipoxygenase.
authors.affiliation: Department of Food Science and technology, The University of Reading, P.O. Box 226, Whiteknights, Reading RG6 6AP, UK.
authors.forename: R
authors.initials: R
authors.lastname: Tangwongchai
authors.forename: D A
authors.initials: DA
authors.lastname: Ledward
authors.forename: J M
authors.initials: JM
authors.lastname: Ames
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D008084:Lipoxygenase / Q000378:metabolism*; D018551:Solanum lycopersicum / Q000737:chemistry / Q000201:enzymology*; D011312:Pressure; D013025:Glycine max / Q000201:enzymology; D013649:Taste
chemical_list: D008084:Lipoxygenase