Functional inactivation of the IGF-I receptor delays differentiation of skeletal muscle cells.
Skeletal myoblasts are inherently programmed to leave the cell cycle and begin the differentiation process following removal of exogenous growth factors. Serum withdrawal results in a marked induction of IGF production which is essential for skeletal muscle differentiation in vitro. However, the potential role of the tyrosine kinase IGF-I receptor (thought to be the principal mediator of both IGF-I and II signaling in skeletal muscle) in the decision of myoblasts to begin differentiation following serum withdrawal is unknown. To explore the role of the IGF-I receptor in this decision by skeletal myoblasts, we functionally inactivated endogenous IGF-I receptors in mouse C2C12 cells using a dominant negative, kinase-inactive IGF-I receptor in which the ATP-binding site lysine (K) at residue 1003 has been mutated to alanine (A). Cell lines with the greatest degree of mutant IGF-I receptor expression (A/K cells) demonstrated functional inactivation of endogenous IGF-I receptors as determined by their impaired ability to phosphorylate the principal substrate of the IGF-I receptor, IRS-1, in response to treatment with IGF-I. In addition, the proliferative response of myoblasts to IGF-I was completely abolished in A/K cells. Following withdrawal of exogenous growth factors, A/K cells demonstrated a marked delay in the induction of the gene expression of myogenin, a skeletal muscle-specific transcription factor essential for differentiation, and a subsequent delay in the induction of muscle creatine kinase activity. Delayed differentiation in A/K cells was associated with prolonged phosphorylation of the cell cycle regulatory retinoblastoma (Rb) protein; it is the un- (or hypo-) phosphorylated form of Rb which is known to promote differentiation in skeletal myoblasts. Thus, the IGF-I receptor regulates the timing of myoblast differentiation induced by serum withdrawal. The delayed differentiation of skeletal myoblasts with functionally inactive IGF-I receptors may result, at least in part, from delayed induction of myogenin gene expression and prolonged phosphorylation of the Rb protein.
Cheng
Z Q
ZQ
Department of Pediatrics, University of California, San Francisco, California 94143, USA.
Adi
S
S
Wu
N Y
NY
Hsiao
D
D
Woo
E J
EJ
Filvaroff
E H
EH
Gustafson
T A
TA
Rosenthal
S M
SM
D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D016895:Culture Media, Serum-Free; D051379:Mice; D018482:Muscle, Skeletal / Q000166:cytology*; D010766:Phosphorylation; D017526:Receptor, IGF Type 1 / Q000502:physiology*; D016160:Retinoblastoma Protein / Q000378:metabolism; D014162:Transfection
D016895:Culture Media, Serum-Free; D016160:Retinoblastoma Protein; D017526:Receptor, IGF Type 1