Comparative quantitative analysis of ultraviolet B-induced skin blood flow change using laser Doppler perfusion imaging technique.
Laser Doppler perfusion imaging (LDI), recently developed, can generate a color-coded image of tissue perfusion, making it possible to assess the spatial distribution of skin perfusion without touching the surface. Using this apparatus, we investigated ultraviolet B (UVB)-induced perfusion profile of the skin and compared the results with those obtained from laser Doppler flowmetry (LDF). Fifteen adult male Korean volunteers were irradiated with several doses of UVB ranging from 25 mJ/cm2 to 186 mJ/cm2. Twenty-four hours later, the erythema reaction was evaluated with LDF and LDI systems. There was a significant correlation between the logarithmic dose of UVB and erythema values. The curves consisted of two parts, an initial, flat phase and then a linear, steep one. Also, there was a good correlation between LDF and LDI. The LDI is as sensitive as conventional LDF, but has the many advantages of measuring blood flow over large areas without contact with the skin surface. This instrument will be useful in the measurement of skin blood flow in many areas of dermatological application.
Youn
J I
JI
Department of Dermatology, Seoul National University College of Medicine and Clinical Research Institute, Seoul National University Hospital, Korea.
Park
S B
SB
Park
B S
BS
Han
W S
WS
D000328:Adult; D003116:Color; D004307:Dose-Response Relationship, Radiation; D004890:Erythema; D006801:Humans; D017078:Laser-Doppler Flowmetry; D016014:Linear Models; D008297:Male; D012039:Regional Blood Flow; D012867:Skin / Q000098:blood supply* / Q000528:radiation effects*; D014466:Ultraviolet Rays