A novel chimeric mitochondrial RNA localized in the nucleus of mouse sperm.
Six identical cDNA clones corresponding to an RNA of 1685 nucleotides that is enriched in mouse sperm compared with testis were isolated from a mouse testis cDNA library. The sequence of these clones corresponds to the 16S mitochondrial RNA plus an inverted repeat of 120 bp covalently joined to the 5' end of the RNA. By RT-PCR, it was demonstrated that this transcript, referred to as chimeric RNA, was present in mouse sperm, testis, liver, kidney, brain, and spleen. The absence of an equivalent sequence in mitochondrial DNA or as a mitochondrial pseudogene in total DNA extracted from sperm, testis, and somatic tissues suggests that the chimeric RNA is a post-transcriptional product, maybe resulting from a trans splicing reaction. The chimeric RNA was found by RT-PCR in total RNA extracted from purified sperm heads. This result was confirmed by in situ hybridization, which showed clear staining of the sperm nucleus with probes corresponding to sequences of the mitochondrial 16S RNA and the inverted repeat.
Villegas
J
J
Bios Chile Ingeniería Genética S.A., Institute for Fundamental and Applied Biology and Fundación Ciencia Para La Vida, Nuñoa, Santiago, Chile.
Zárraga
A M
AM
Muller
I
I
Montecinos
L
L
Werner
E
E
Brito
M
M
Meneses
A M
AM
Burzio
L O
LO
D000818:Animals; D001483:Base Sequence; D002678:Chimera; D018076:DNA, Complementary; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D012313:RNA / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000077278:RNA, Mitochondrial; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013094:Spermatozoa / Q000378:metabolism*
D018076:DNA, Complementary; D012333:RNA, Messenger; D000077278:RNA, Mitochondrial; D012313:RNA