A delta opioid receptor lacking the third cytoplasmic loop is generated by atypical mRNA processing in human malignomas.
delta Opioid receptors were identified in human melanomas by RT-PCR and radioligand binding. In all tumors an additional PCR amplificate was detected in which 144 bp within the third exon were deleted. This fragment corresponded to the third cytoplasmic domain of the receptor protein. The short variant resulted from atypical mRNA processing. There were no common splice recognition sequences around the deleted fragment; instead its excision resembled the removal of a transposon. The deletion was not detected in normal human melanocytes nor in human or rat brain. However, it was present in a human neuroblastoma cell line (SH-SY5Y). Thus, it appears that the occurrence of the short delta opioid receptor is correlated to malignancy.
Mayer
P
P
Institute for Pharmacology and Toxicology, Otto von Guericke University of Magdeburg, Germany.
Tischmeyer
H
H
Jayasinghe
M
M
Bonnekoh
B
B
Gollnick
H
H
Teschemacher
H
H
Höllt
V
V
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism; D018076:DNA, Complementary; D006801:Humans; D008544:Melanocytes / Q000166:cytology; D008545:Melanoma; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D010860:Pigments, Biological; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger; D051381:Rats; D017465:Receptors, Opioid, delta / Q000235:genetics* / Q000378:metabolism; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D010860:Pigments, Biological; D012333:RNA, Messenger; D017465:Receptors, Opioid, delta