Anti-cathepsin G antibodies in the sera of patients with ulcerative colitis.
The presence of perinuclear anti-neutrophil cytoplasmic antibodies (P-ANCAs) and that of antibodies against cathepsin G, a target antigen for P-ANCAs, was determined in the sera of patients with ulcerative colitis (UC), relative to the endoscopic severity and disease activity. P-ANCAs were detected by indirect immunofluorescent assay (IIF) on ethanol-fixed human neutrophils. Antibodies to cathepsin G were detected by an enzyme-linked immunosorbent assay (ELISA) and Western blotting. P-ANCAs were detected by IIF in 62.5% of 32 patients with active UC. Anti-cathepsin G antibodies were detected in 40.6% of 32 patients with active UC, and their prevalence was significantly higher in patients with severe colitis, as determined by endoscopy, than in those with mild or moderate colitis (P < 0.05). The prevalence and titers of anti-cathepsin G antibodies were significantly higher during the active than the inactive phase of the disease (P < 0.05). Measurement of titers of anti-cathepsin G antibodies by ELISA in the serum is useful for evaluating the activity of UC.
Kuwana
T
T
Second Department of Internal Medicine, Fukushima Medical University School of Medicine, Hikarigaoka, Japan.
Sato
Y
Y
Saka
M
M
Kondo
Y
Y
Miyata
M
M
Obara
K
K
Nishimaki
T
T
Kasukawa
R
R
D000293:Adolescent; D000328:Adult; D000368:Aged; D019268:Antibodies, Antineutrophil Cytoplasmic / Q000097:blood*; D001323:Autoantibodies / Q000097:blood*; D015153:Blotting, Western; D056649:Cathepsin G; D002403:Cathepsins / Q000276:immunology* / Q000378:metabolism; D003093:Colitis, Ulcerative / Q000097:blood / Q000145:classification / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D008297:Male; D008875:Middle Aged; D015995:Prevalence; D012697:Serine Endopeptidases; D012720:Severity of Illness Index
D019268:Antibodies, Antineutrophil Cytoplasmic; D001323:Autoantibodies; D002403:Cathepsins; D012697:Serine Endopeptidases; C534094:CTSG protein, human; D056649:Cathepsin G