title: Detection of circulating lipopolysaccharide-bound monocytes in children with gram-negative sepsis.
abstract: The possibility that gram-negative sepsis can be diagnosed by detection of lipopolysaccharide (LPS) bound on the surface of monocytes in the circulation of patients with gram-negative sepsis was investigated. Peripheral monocytes were analyzed by flow cytometer and an anti-LPS monoclonal antibody in 3 groups: children with gram-negative sepsis, children with gram-positive sepsis, and healthy children. LPS-bound monocytes were found in all patients with gram-negative sepsis but not in children with gram-positive sepsis or in healthy children. Therefore, the flow cytometry method developed for this study may be a novel method for diagnosing gram-negative sepsis.
authors.affiliation: Department of Pediatrics, National Defense Medical College, Tokorozawa, Saitama 359-8513, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Takeshita
authors.forename: K
authors.initials: K
authors.lastname: Nakatani
authors.forename: H
authors.initials: H
authors.lastname: Tsujimoto
authors.forename: Y
authors.initials: Y
authors.lastname: Kawamura
authors.forename: I
authors.initials: I
authors.lastname: Sekine
mesh_terms: D000328:Adult; D016470:Bacteremia / Q000097:blood*; D016905:Gram-Negative Bacterial Infections / Q000097:blood*; D006801:Humans; D007223:Infant; D018950:Lipopolysaccharide Receptors / Q000097:blood; D008070:Lipopolysaccharides / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism*
chemical_list: D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides