A pot-pourri of plasmid paradoxes: effects of a second copy.
Bacterial plasmids are exemplary subjects for study, being conveniently isolated, dissected, reassembled, and introduced into various hosts. Their versatility and power make them eminently worthy of our attention. In what follows I consider some consequences of simply doubling the dosage of particular plasmid genes or of forming a plasmid dimer. These consequences can be perverse, paradoxical, or informative. They bear on questions of cell viability, copy number limitation, clonal homogeneity, check-point control, and the recovery of mutants. They have relevance to biotechnology, evolution and medicine. In reviewing these effects, my motivation is largely to share my enthusiasm for certain kinds of biological narratives, the nature of which is best left for the reader to discern.
Yarmolinsky
M B
MB
Laboratory of Biochemistry, National Cancer Institute, N. I. H., 37 Convent Drive, Bethesda, MD 20892-4255, USA. myarmo@helix.nih.gov
D001419:Bacteria / Q000235:genetics; D004261:DNA Replication; D009154:Mutation; D010957:Plasmids