title: Binding of filamin isoforms to myofibrils.
abstract: Two filamin isoforms were purified from bovine tissues and characterized. Muscle filamin and nonmuscle filamin had different SDS gel mobilities, proteolytic digestion patterns, myofibrillar binding distributions and myofibril binding affinities. The muscle specific filamin had an apparent molecular weight of 265 kDa and bound primarily to the Z-lines of myofibrils but also to the I-bands near the Z-lines. The nonmuscle specific filamin had an apparent molecular weight of 275 kDa and bound exclusively to the Z-lines of myofibrils. The filamin myofibril binding was studied quantitatively. Plotting bound fraction (mg filamin/mg myofibril) vs. equilibrium concentration of free filamin yielded a biphasic binding curve. The first hyperbolic binding phase described the binding of filamin to myofibrils but the second phase appeared to be nonspecific due to filamin aggregation. The muscle filamin had a significantly lower (P < 0.05) apparent binding affinity to myofibrils than nonmuscle filamin. However, the muscle filamin showed a significantly higher (P < 0.05) saturation value for myofibrils than nonmuscle filamin. The binding of phosphorylated filamin to myofibrils was significantly lower (P < 0.05) than the corresponding native proteins for both filamin isoforms.
authors.affiliation: Muscle Biology Laboratory, University of Wisconsin-Madison, 53706, USA.
authors.forename: W
authors.initials: W
authors.lastname: Chiang
authors.forename: M L
authors.initials: ML
authors.lastname: Greaser
mesh_terms: D000818:Animals; D002417:Cattle; D002645:Chickens; D003285:Contractile Proteins / Q000302:isolation & purification / Q000378:metabolism*; D064448:Filamins; D005895:Gizzard, Avian / Q000737:chemistry; D015151:Immunoblotting; D007700:Kinetics; D051379:Mice; D008840:Microfilament Proteins / Q000302:isolation & purification / Q000378:metabolism*; D009210:Myofibrils / Q000378:metabolism*; D009928:Organ Specificity; D011485:Protein Binding; D020033:Protein Isoforms / Q000378:metabolism
chemical_list: D003285:Contractile Proteins; D064448:Filamins; D008840:Microfilament Proteins; D020033:Protein Isoforms