title: Extracellular FGF-1 inhibits cytoskeletal organization and promotes fibroblast motility.
abstract: Previous efforts from this laboratory have established that acidic fibroblast growth factor (FGF-1), either added exogenously or secreted as a biologically active protein, induces a transformed phenotype in primary murine fibroblasts. Experimental studies described here demonstrate that constitutive exposure to extracellular FGF-I results in reduced cell attachment to multiple ligands, inhibition of cytoskeletal organization, and reduced collagen contraction, despite no detectable change in integrin cell surface expression. In addition, FGF-1-transduced fibroblasts demonstrated a > 10-fold increase in migration, an observation correlated with increased tyrosine phosphorylation of p125FAK and p130CAS. Collectively, these results suggest that FGF-1-induced fibroblast transformation includes the involvement of specific FGF receptor-mediated signal transduction cascades targeted to cytoskeletal and focal adhesion structures.
authors.affiliation: Department of Surgery, School of Medicine, University of Alabama at Birmingham, 35294, USA.
authors.forename: Q
authors.initials: Q
authors.lastname: Ding
authors.forename: C L
authors.initials: CL
authors.lastname: Gladson
authors.forename: C R
authors.initials: CR
authors.lastname: Guidry
authors.forename: S A
authors.initials: SA
authors.lastname: Santoro
authors.forename: S K
authors.initials: SK
authors.lastname: Dickeson
authors.forename: J T
authors.initials: JT
authors.lastname: Shin
authors.forename: J A
authors.initials: JA
authors.lastname: Thompson
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects*; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced*; D003599:Cytoskeleton / Q000187:drug effects*; D016220:Fibroblast Growth Factor 1; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D005347:Fibroblasts; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D016220:Fibroblast Growth Factor 1; D005346:Fibroblast Growth Factors