Bisphenol A diglycidyl ether (BADGE) is a PPARgamma agonist in an ECV304 cell line.
Peroxisome proliferator activated receptors (PPAR)s are nuclear transcription factors of the steroid receptor super-family. One member, PPARgamma, a critical transcription factor in adipogenesis, is expressed in ECV304 cells, and when activated participates in the induction of cell death by apoptosis. Here we describe a clone of ECV304 cells, ECV-ACO.Luc, which stably expresses a reporter gene for PPAR activation. ECV-ACO.Luc respond to the PPARgamma agonists, 15-deoxy-Delta(12,14) PGJ(2), and ciglitizone, by inducing luciferase expression. Furthermore, using ECV-ACO.Luc, we demonstrate that a newly described PPARgamma antagonist, bisphenol A diglycidyl ether (BADGE) has agonist activities. Similar to 15-deoxy-Delta(12,14) PGJ(2), BADGE induces PPARgamma activation, nuclear localization of the receptor, and induces cell death.
Bishop-Bailey
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Department of Cardiac, Vascular and Inflammation Research, William Harvey Research Institute, St. Bartholomew's and the Royal London School of Medicine and Dentistry, Charterhouse Square, London, EC1M 6BQ, UK. d.bishop-bailey@mds.qmw.ac.uk
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Warner
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D001559:Benzhydryl Compounds; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D002467:Cell Nucleus / Q000737:chemistry; D004852:Epoxy Compounds / Q000494:pharmacology*; D006801:Humans; D008156:Luciferases / Q000235:genetics; D018160:Receptors, Cytoplasmic and Nuclear / Q000819:agonists* / Q000032:analysis / Q000502:physiology; D014157:Transcription Factors / Q000819:agonists* / Q000032:analysis / Q000502:physiology; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D004852:Epoxy Compounds; D008156:Luciferases; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors; C019273:bisphenol A diglycidyl ether; D000098826:Bisphenol A Compounds