The new serine-threonine kinase, Qik, is a target of the Qin oncogene.
The winged helix transcription factor Qin is the avian homolog of the mammalian brain factor 1 (BF-1) and has the potential to act as an oncogenic protein. We used representational difference analysis to identify genes that are differentially expressed in chicken embryo fibroblasts (CEF) transformed by Qin. One of the up-regulated Qin targets identified in this analysis is a serine-threonine kinase termed Qik (Qin-induced kinase). Qik belongs to the AMPK/SNF1 kinase family. It is a ubiquitously expressed protein and is upregulated rapidly after a hormone-regulated form of Qin is activated. In vitro kinase tests demonstrate that Qik is capable of autophosphorylation. Elevated levels of Qik transcripts are also observed in Src-transformed cells, suggesting that Src and Qin share some targets.
Xia
Y
Y
Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, BCC239, La Jolla, California 92037, USA.
Zhang
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Z
Kruse
U
U
Vogt
P K
PK
Li
J
J
D000595:Amino Acid Sequence; D000818:Animals; D030161:Avian Proteins; D001483:Base Sequence; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D002642:Chick Embryo; D018076:DNA, Complementary / Q000032:analysis; D005347:Fibroblasts / Q000378:metabolism / Q000502:physiology; D051858:Forkhead Transcription Factors; D016391:Genes, src / Q000502:physiology; D008969:Molecular Sequence Data; D015513:Oncogene Proteins; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D011518:Proto-Oncogene Proteins / Q000502:physiology*; D017386:Sequence Homology, Amino Acid; D015854:Up-Regulation; D014764:Viral Proteins
D030161:Avian Proteins; D018076:DNA, Complementary; D051858:Forkhead Transcription Factors; D015513:Oncogene Proteins; D011518:Proto-Oncogene Proteins; D014764:Viral Proteins; C490425:v-qin protein, Avian sarcoma virus 31; C071570:SNF1-related protein kinases; D017346:Protein Serine-Threonine Kinases