title: VP4 and VP7 genotyping by reverse transcription-PCR of human rotavirus in mexican children with acute diarrhea.
abstract: Dual typing (VP4 and VP7) of rotavirus obtained from 257 Mexican children during three epidemiological seasons was performed by reverse transcription-PCR. The P1G1 genotype was the most prevalent (40%), followed by P1G3 (19%) and P2G2 (16%). Thirty-one specimens (12%) presented mixed infections, while some genotypes were not found. This is the first dual typing of isolates from diarrhea cases in Mexico.
authors.affiliation: Laboratorio de Rotavirus, Instituto Nacional de Diagnóstico y Referencia Epidemiológicos (INDRE), Secretaría de Salud, Mexico City, Mexico.
authors.forename: A
authors.initials: A
authors.lastname: Rodríguez Castillo
authors.forename: A V
authors.initials: AV
authors.lastname: Villa
authors.forename: J E
authors.initials: JE
authors.lastname: Ramírez González
authors.forename: E
authors.initials: E
authors.lastname: Mayén Pimentel
authors.forename: M
authors.initials: M
authors.lastname: Melo Munguía
authors.forename: B
authors.initials: B
authors.lastname: Díaz De Jesús
authors.forename: H
authors.initials: H
authors.lastname: Olivera Díaz
authors.forename: H
authors.initials: H
authors.lastname: García Lozano
mesh_terms: D000208:Acute Disease; D000956:Antigens, Viral; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D002648:Child; D003967:Diarrhea / Q000453:epidemiology / Q000821:virology*; D005838:Genotype; D006801:Humans; D008800:Mexico / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction; D021521:Ribotyping; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000175:diagnosis* / Q000453:epidemiology
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; C060339:VP4 protein, Rotavirus; C050779:VP7 protein, Rotavirus