Uracil incorporation into a gene targeting construct reduces the frequency of homologous and nonhomologous recombinants in human cells.
Gene targeting allows the introduction of specific modifications into the eukaryotic genome by homologous recombination, but its efficiency is low in many mammalian systems. We are exploring different ways to increase the efficiency of gene targeting and we report here the effect of uracil incorporation in the targeting construct. Plasmids containing uracil substituting for a fraction of thymine residues are hyperrecombinogenic in some bacterial systems. To test whether a similar stimulation of recombination occurs in mammalian cells, we have prepared a uracil-rich HPRT targeting construct and quantified its homologous and nonhomologous recombination frequencies compared to the same plasmid lacking uracil. The uracil-rich plasmid led to reductions in both homologous and nonhomologous recombination in human cells.
Yáñez
R J
RJ
Gene Targeting Group, MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital, London W12 0NN, UK. rafael.yanez@csc.mrc.ac.uk
Porter
A C
AC
D000704:Analysis of Variance; D018390:Gene Targeting; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011995:Recombination, Genetic; D014407:Tumor Cells, Cultured; D014498:Uracil / Q000378:metabolism*
D014498:Uracil; D007041:Hypoxanthine Phosphoribosyltransferase