title: Structural changes in cytochrome c oxidase induced by cytochrome c binding. A resonance raman study.
abstract: Electrostatically stabilized complexes of fully oxidized cytochrome c oxidase from Paracoccus denitrificans and horse heart cytochrome c were studied by resonance Raman spectroscopy. The experiments were carried out with the wild-type oxidase and a variant in which a negatively charged amino acid in the binding domain (D257) is replaced by an asparagine. It is shown that cytochrome c induces structural changes at heme a and heme a(3) which are reminiscent to those found in mammalian cytochrome c oxidase-cytochrome c complex. The spectral changes are attributed to subtle changes in the heme-protein interactions implying that there is a structural communication from the binding domain even to the remote catalytic center. Only for the heme a modes minor spectral differences were found in the response of the wild-type and the D257N variant oxidase upon cytochrome c binding indicating that electrostatic interactions of aspartate 257 are not crucial for the perturbation of the catalytic site structure in the complex. On the other hand, in none of the complexes, structural changes were detected in the bound cytochrome c. These findings are in contrast to previous results obtained with beef heart cytochrome c oxidase which triggers the formation of a new conformational state of cytochrome c assumed to be involved in the biological electron transfer process.
authors.affiliation: Max-Planck-Institut für Strahlenchemie, Mülheim an der Ruhr, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Döpner
authors.forename: J
authors.initials: J
authors.lastname: Hudecek
authors.forename: B
authors.initials: B
authors.lastname: Ludwig
authors.forename: H
authors.initials: H
authors.lastname: Witt
authors.forename: P
authors.initials: P
authors.lastname: Hildebrandt
mesh_terms: D000818:Animals; D003574:Cytochrome c Group / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000737:chemistry* / Q000378:metabolism; D006736:Horses; D011485:Protein Binding; D013059:Spectrum Analysis, Raman
chemical_list: D003574:Cytochrome c Group; D003576:Electron Transport Complex IV