Targeting of phage display vectors to mammalian cells.
Phage display libraries offer a strategy to isolate peptide ligands to target proteins and to define potential interaction sites between proteins. Recent studies have indicated a novel utility for phage display in that bacteriophage engineered to express peptide ligands to specific cell surface receptors are internalized by mammalian cells. Thus, reporter genes such as green fluorescent protein and lacZ harbored in the phage genome can be delivered to mammalian cells using targeting peptides displayed on the surface of phage. There is also the possibility to generate novel types of peptide libraries expressed intracellularly using a phage capable of inducing expression of its coding genes in human cells.
Uppala
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A
University of Helsinki, Department of Biosciences, Division of Biochemistry, Viikinkaari 5, Helsinki, FIN-00014, Finland.
Koivunen
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E
D000818:Animals; D000954:Antigens, Surface; D001435:Bacteriophages / Q000235:genetics; D002213:Capsid / Q000235:genetics; D004705:Endocytosis; D005544:Forecasting; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006801:Humans; D008322:Mammals; D019151:Peptide Library
D000954:Antigens, Surface; D019151:Peptide Library