title: Production of B-group vitamins by two Azotobacter strains with phenolic compounds as sole carbon source under diazotrophic and adiazotrophic conditions.
abstract: Azotobacter vinelandii strain ATCC 12837 and A. chroococcum strain H23 (CECT 4435) were able to grow on N-free or NH4Cl-amended chemically-defined (Burk's) media, with protocatechuic acid (1-2 mmol 1(-1)) or sodium p-hydroxybenzoate (1-10 mmol 1(-1)) as sole carbon (C) sources. At a concentration of 2 mmol 1(-1), both substrates supported nitrogen fixation (acetylene reduction assay) at similar or higher rates than bacteria grown in control media amended with 2 mmol 1(-1) sodium succinate as C source. The two strains produced the B-group vitamins niacin, pantothenic acid, thiamine, riboflavin and biotin after 72 h of growth in chemically-defined media with 2 mmol 1(-1) protocatechuic acid, sodium phydroxybenzoate or sodium succinate as sole C source, either in N-free media or in media amended with 0.1% NH4Cl. Quantitative production of all vitamins was affected by the use of the different C and N substrates.
authors.affiliation: Departamento de Microbiología, Universidad de Granada, Spain.
authors.forename: J J
authors.initials: JJ
authors.lastname: Revillas
authors.forename: B
authors.initials: B
authors.lastname: Rodelas
authors.forename: C
authors.initials: C
authors.lastname: Pozo
authors.forename: M V
authors.initials: MV
authors.lastname: Martínez-Toledo
authors.forename: J
authors.initials: J
authors.lastname: González-López
mesh_terms: D000643:Ammonium Chloride / Q000378:metabolism; D001395:Azotobacter / Q000378:metabolism*; D001565:Benzoates / Q000378:metabolism; D001710:Biotin / Q000096:biosynthesis; D003470:Culture Media; D062385:Hydroxybenzoates / Q000378:metabolism; D009525:Niacin / Q000096:biosynthesis; D010205:Pantothenic Acid / Q000096:biosynthesis; D012256:Riboflavin / Q000096:biosynthesis; D013386:Succinates / Q000378:metabolism; D013831:Thiamine / Q000096:biosynthesis; D013997:Time Factors; D014803:Vitamin B Complex / Q000096:biosynthesis*
chemical_list: D001565:Benzoates; D003470:Culture Media; D062385:Hydroxybenzoates; D013386:Succinates; D000643:Ammonium Chloride; D014803:Vitamin B Complex; D010205:Pantothenic Acid; D009525:Niacin; C009091:protocatechuic acid; D001710:Biotin; D012256:Riboflavin; D013831:Thiamine