Identification of a new HLA-B*39 allele: HLA-B*3924.
We have identified a new HLA-B*39 allele through polymerase chain reaction (PCR) using sequence-specific primers (SSP) and sequence-based typing of exons 2 and 3. This novel allele was identified in three HLA-identical siblings of Turkish origin. This allele only differs from HLA-B*3903 at a unique single nucleotide substitution (T for C) at position 365 in exon 3 which results in an amino acid change in codon 98 of methionine (ATG) to threonine (ACG). The sequencing enabled the development of a monospecific PCR-SSP reaction which can be used to discriminate between HLA-B*3924 and other B*39 alleles.
Cook
J T
JT
Nuffield Department of Surgery, Oxford Transplant Centre, Churchill Hospital, United Kingdom. jtcook@hgmp.mrc.ac.uk
Day
S
S
Dunn
P P
PP
Welsh
K I
KI
Bunce
M
M
D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers; D005091:Exons; D015235:HLA-B Antigens / Q000235:genetics* / Q000276:immunology; D059946:HLA-B39 Antigen; D006801:Humans; D008969:Molecular Sequence Data; D017354:Point Mutation / Q000276:immunology; D016133:Polymerase Chain Reaction; D014421:Turkey
D017931:DNA Primers; D015235:HLA-B Antigens; D059946:HLA-B39 Antigen