title: Increased expression of osteopontin in activated Kupffer cells and hepatic macrophages during macrophage migration in Propionibacterium acnes-treated rat liver.
abstract: Osteopontin is an extracellular matrix component that can act as a chemokine to induce macrophage migration. The significance of osteopontin in macrophage infiltration into the liver was examined in rats given heat-killed Propionibacterium acnes. In normal rats, osteopontin mRNA expression in the liver was minimal, determined by quantitative-competitive reverse transcription-polymerase chain reaction (RT-PCR) assay. Northern blot analysis revealed that osteopontin mRNA was not expressed in Kupffer cells isolated from normal rats. When rats received heat-killed P. acnes intravenously, marked macrophage accumulation, forming granulomas, developed in the liver later than 3 days after the injection and its extent became maximal between 5 and 7 days. In these rats, osteopontin mRNA expression was increased in the liver later than 1 day (with its peak at 3 days after the injection), and the mRNA expression was increased markedly in Kupffer cells and hepatic macrophages isolated at 7 days. The mRNA expression of monocyte chemotactic protein-1 (MCP-1) and macrophage inflammatory protein-1alpha (MIP-1alpha), chemokines for monocytes and macrophages, was also increased in the liver of P. acnes-treated rats, with peak expression at 3 days. We conclude that osteopontin derived from Kupffer cells and hepatic macrophages may contribute to the infiltration of monocytes and macrophages into the liver cooperatively with the actions of MCP-1 and MIP-1alpha in P. acnes-treated rats.
authors.affiliation: Third Department of Internal Medicine, Saitama Medical School, Iruma-gun, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: S
authors.initials: S
authors.lastname: Mochida
authors.forename: R
authors.initials: R
authors.lastname: Kawashima
authors.forename: M
authors.initials: M
authors.lastname: Inao
authors.forename: A
authors.initials: A
authors.lastname: Matsui
authors.forename: Y
authors.initials: Y
authors.lastname: YouLuTuZ
authors.forename: S
authors.initials: S
authors.lastname: Nagoshi
authors.forename: T
authors.initials: T
authors.lastname: Uede
authors.forename: K
authors.initials: K
authors.lastname: Fujiwara
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002465:Cell Movement; D018932:Chemokine CCL2 / Q000378:metabolism; D018925:Chemokines / Q000235:genetics / Q000378:metabolism*; D016908:Gram-Positive Bacterial Infections / Q000473:pathology*; D007728:Kupffer Cells / Q000378:metabolism*; D008099:Liver / Q000276:immunology / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism* / Q000502:physiology; D008297:Male; D053495:Osteopontin; D011425:Propionibacterium acnes; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011916:Rats, Inbred F344; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012795:Sialoglycoproteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D018932:Chemokine CCL2; D018925:Chemokines; D012333:RNA, Messenger; D012795:Sialoglycoproteins; C506897:Spp1 protein, rat; D053495:Osteopontin