title: Oral infection of Culicoides sonorensis (Diptera: Ceratopogonidae) by vesicular stomatitis virus.
abstract: Vesicular stomatitis virus serotype New Jersey (VSNJV) was mixed with bovine blood or fetal bovine serum (FBS) and fed across silicone membranes to laboratory populations of Culicoides sonorensis Wirth & Jones. In an initial study, virus was detected after 13 d in 21% of the midges that received an FBS/VSNJV mixture. In subsequent time-course experiments, engorged females were collected and maintained at 20.0 degrees C and assayed for VSNJV immediately after feeding and at 1, 3, 7, 10 and 13 d after feeding. Virus was detected after 13 d in 3% of the midges that received a bovine blood/VSNJV mixture and in 9% of the midges that received an FBS/VSNJV mixture. The results indicate that C. sonorensis should be considered as a potential biological vector of VSNJV.
authors.affiliation: USDA-ARS Arthropod-Borne Animal Diseases Research Laboratory, Laramie, WY 82071, USA.
authors.forename: R A
authors.initials: RA
authors.lastname: Nunamaker
authors.forename: A A
authors.initials: AA
authors.lastname: Peréz De León
authors.forename: C L
authors.initials: CL
authors.lastname: Campbell
authors.forename: S M
authors.initials: SM
authors.lastname: Lonning
mesh_terms: D000818:Animals; D002417:Cattle; D002519:Ceratopogonidae / Q000821:virology*; D005260:Female; D007303:Insect Vectors / Q000821:virology*; D018116:Vesiculovirus