VP4 and VP7 genotyping by reverse transcription-PCR of human rotavirus in mexican children with acute diarrhea.
Dual typing (VP4 and VP7) of rotavirus obtained from 257 Mexican children during three epidemiological seasons was performed by reverse transcription-PCR. The P1G1 genotype was the most prevalent (40%), followed by P1G3 (19%) and P2G2 (16%). Thirty-one specimens (12%) presented mixed infections, while some genotypes were not found. This is the first dual typing of isolates from diarrhea cases in Mexico.
Rodríguez Castillo
A
A
Laboratorio de Rotavirus, Instituto Nacional de Diagnóstico y Referencia Epidemiológicos (INDRE), Secretaría de Salud, Mexico City, Mexico.
Villa
A V
AV
Ramírez González
J E
JE
Mayén Pimentel
E
E
Melo Munguía
M
M
Díaz De Jesús
B
B
Olivera Díaz
H
H
García Lozano
H
H
D000208:Acute Disease; D000956:Antigens, Viral; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D002648:Child; D003967:Diarrhea / Q000453:epidemiology / Q000821:virology*; D005838:Genotype; D006801:Humans; D008800:Mexico / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction; D021521:Ribotyping; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000175:diagnosis* / Q000453:epidemiology
D000956:Antigens, Viral; D036022:Capsid Proteins; C060339:VP4 protein, Rotavirus; C050779:VP7 protein, Rotavirus