Differential responsiveness of prostatic acid phosphatase and prostate-specific antigen mRNA to androgen in prostate cancer cells.
Androgens regulate the expression of both human prostatic acid phosphatase (PAcP) and prostate-specific antigen (PSA), two major prostate epithelium-specific differentiation antigens. Due to the important role of these two enzymes as prostate epithelium differentiation markers, we investigated their regulation of expression at the mRNA level in LNCaP human prostate carcinoma cells. Interestingly, phenol red, a pH indicator in the culture medium, promoted cell growth. To eliminate this non-specific effect, a phenol red-free, steroid-reduced medium was utilized. When high-density cells were grown in that medium, 5alpha-dihydrotestosterone (DHT) suppressed PAcP but stimulated PSA. However, tumor promoter phorbol ester 12-o-tetradecanoyl phorbol-13-acetate (TPA) functioned as a potent inhibitor of both PAcP and PSA expression. Prolonged treatment with DHT as well as TPA resulted in a similar down-regulation of protein kinase C and cellular PAcP activities. Thus, the levels of PAcP and PSA mRNA are differentially regulated by androgens in LNCaP cells.
Lin
M F
MF
Department of Biochemistry and Molecular Biology, College of Medicine, University of Nebraska Medical Center, Omaha 68198, USA. mlin@unmc.edu
Lee
M S
MS
Garcia-Arenas
R
R
Lin
F F
FF
D000135:Acid Phosphatase / Q000187:drug effects* / Q000378:metabolism; D000728:Androgens / Q000494:pharmacology; D002273:Carcinogens / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D013196:Dihydrotestosterone / Q000494:pharmacology; D006801:Humans; D008297:Male; D010637:Phenolsulfonphthalein / Q000494:pharmacology; D011467:Prostate / Q000187:drug effects* / Q000378:metabolism; D017430:Prostate-Specific Antigen / Q000187:drug effects* / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000378:metabolism*; D011493:Protein Kinase C / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000187:drug effects* / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000728:Androgens; D002273:Carcinogens; D012333:RNA, Messenger; D013196:Dihydrotestosterone; D011493:Protein Kinase C; D000135:Acid Phosphatase; D017430:Prostate-Specific Antigen; D010637:Phenolsulfonphthalein; D013755:Tetradecanoylphorbol Acetate