A standardized spectrophotometric assay of endoglycanase activities using dyed, amorphous polysaccharides.
This is a new technique to assay virtually any endoglycanase activity where enough polysaccharide material is available to allow for production of the amorphous, dyed beads used as substrates. It allows for a direct comparison of endoglycanase activities between laboratories since dyed beads from at least the most common polysaccharides such as cellulose, xylan, mannan, and chitin are now under development and will soon be commercially available; cellulose beads already are. It is a very sensitive technique and enzyme activities can be measured using a nonsophisticated spectrophotometer.
Pettersson
B
B
STFI, Swedish Pulp and Paper Research Institute, SE-114 86 Stockholm, Sweden. bert.pettersson@stfi.se
Eriksson
K E
KE
D000880:Anthraquinones; D002482:Cellulose / Q000737:chemistry*; D004396:Coloring Agents; D005947:Glucose / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000032:analysis*; D008863:Microspheres; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods*
D000880:Anthraquinones; D004396:Coloring Agents; D002482:Cellulose; D006026:Glycoside Hydrolases; D005947:Glucose; C024758:Remazol Brilliant Blue R