Immediate contact hypersensitivity induced by repeated hapten challenge in mice.
An immediate reaction was investigated during repeated challenge testing for contact hypersensitivity to dinitrofluorobenzene (DNFB) in BALB/c mice. The mice were sensitized to DNFB on back skin and repeatedly challenged with the same hapten on the left ear at 1 week intervals. The ear after the 5th challenge showed biphasic responses which consisted of an immediate and a delayed-type reaction. The reactions were hapten specific. Mast cell-deficient WBB6F1 W/WV mice did not show any immediate reaction, while congenic normal mice showed both immediate and delayed-type reactions. Histologically, numerous dermal mast cells were found in the left ear of repeatedly challenged BALB/c and WBB6F1 normal mice, while there were few mast cells in the ear of WBB6F1 W/WV mice. Anti-DNP IgE antibodies were detected in BALB/c, WBB6F1 normal and W/WV mice after repeated challenge with DNFB. Intradermal injection of anti-IgE antibodies in the repeatedly DNFB-challenged ear elicited an immediate reaction. These results suggest that immediate contact hypersensitivity develops through the production of anti-DNP IgE antibodies and an increase in dermal mast cells after repeated challenge with DNFB.
Natsuaki
M
M
Department of Dermatology, Hyogo College of Medicine, Nishinomiya, Japan.
Yano
N
N
Yamaya
K
K
Kitano
Y
Y
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D017449:Dermatitis, Allergic Contact / Q000175:diagnosis / Q000276:immunology*; D004139:Dinitrofluorobenzene; D004195:Disease Models, Animal; D005260:Female; D006241:Haptens; D006969:Hypersensitivity, Immediate / Q000139:chemically induced / Q000276:immunology*; D007073:Immunoglobulin E / Q000032:analysis* / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis* / Q000276:immunology; D008407:Mast Cells / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011336:Probability; D012680:Sensitivity and Specificity; D012882:Skin Tests
D000888:Antibodies, Anti-Idiotypic; D006241:Haptens; D007074:Immunoglobulin G; D007073:Immunoglobulin E; D004139:Dinitrofluorobenzene