The FMRFamide-like neuropeptide AF2 is present in the parasitic nematode Haemonchus contortus.
Peptides belonging to the FMRFamide family are widely distributed amongst invertebrates. We report here on the isolation of the FMRFamide neuropeptide AF2 (Lys-His-Glu-Tyr-Leu-Arg-Phe-NH2) from the parasitic nematode Haemonchus contortus. Immunocytochemical techniques showed that FMRFamide-like material was distributed in several regions of these organisms including nerve cords and cell bodies of the central nervous system. AF2 was isolated using a method that employed 6 steps of reverse-phase HPLC. The concentration of AF2 in this organism was approximately 30 pmol/g of nematode.
Keating
C D
CD
Department of Physiology and Pharmacology, University of Southampton, Bassett Crescent East.
Holden-Dye
L
L
Thorndyke
M C
MC
Williams
R G
RG
Mallett
A
A
Walker
R J
RJ
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D001667:Binding, Competitive; D006189:Haemonchus / Q000737:chemistry*; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D009420:Nervous System / Q000737:chemistry*; D009479:Neuropeptides / Q000276:immunology / Q000302:isolation & purification*; D017421:Sequence Analysis
D009479:Neuropeptides; C073964:AF2 neuropeptide