title: New molecular aspects of regulation of mitochondrial activity by fenofibrate and fasting.
abstract: Fenofibrate and fasting are known to regulate several genes involved in lipid metabolism in a similar way. In this study measuring several mitochondrial enzyme activities, we demonstrate that, in contrast to citrate synthase and complex II, cytochrome c oxidase (COX) is a specific target of these two treatments. In mouse liver organelles, Western blot experiments indicated that mitochondrial levels of p43, a mitochondrial T3 receptor, and mitochondrial peroxisome proliferator activated receptor (mt-PPAR), previously described as a dimeric partner of p43 in the organelle, are increased by both fenofibrate and fasting. In addition, in PPAR alpha-deficient mice, this influence was abolished for mt-PPAR but not for p43, whereas the increase in COX activity was not altered. These data indicate that: (1) PPAR alpha is involved in specific regulation of mt-PPAR expression by both treatments; (2) fenofibrate and fasting regulate the mitochondrial levels of p43 and thus affect the efficiency of the direct T3 mitochondrial pathway.
authors.affiliation: UMR Différenciation Cellulaire et Croissance (INRA-UMII-ENSAM), Unité d'Endocrinologie Cellulaire, Institut National de la Recherche Agronomique (INRA) 34060 Montpellier Cedex 1, France.
authors.forename: F
authors.initials: F
authors.lastname: Casas
authors.forename: T
authors.initials: T
authors.lastname: Pineau
authors.forename: P
authors.initials: P
authors.lastname: Rochard
authors.forename: A
authors.initials: A
authors.lastname: Rodier
authors.forename: L
authors.initials: L
authors.lastname: Daury
authors.forename: M
authors.initials: M
authors.lastname: Dauça
authors.forename: G
authors.initials: G
authors.lastname: Cabello
authors.forename: C
authors.initials: C
authors.lastname: Wrutniak-Cabello
mesh_terms: D000818:Animals; D002950:Citrate (si)-Synthase / Q000378:metabolism; D003433:Crosses, Genetic; D004268:DNA-Binding Proteins / Q000378:metabolism; D019281:Dimerization; D042963:Electron Transport Complex II; D003576:Electron Transport Complex IV / Q000378:metabolism; D005215:Fasting; D011345:Fenofibrate / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008930:Mitochondria, Liver / Q000187:drug effects / Q000201:enzymology / Q000502:physiology*; D009097:Multienzyme Complexes / Q000378:metabolism; D015388:Organelles / Q000187:drug effects / Q000502:physiology; D010088:Oxidoreductases / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D013385:Succinate Dehydrogenase / Q000378:metabolism; D014157:Transcription Factors / Q000172:deficiency / Q000235:genetics / Q000502:physiology*
chemical_list: D004268:DNA-Binding Proteins; D009097:Multienzyme Complexes; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors; D010088:Oxidoreductases; D042963:Electron Transport Complex II; D013385:Succinate Dehydrogenase; D003576:Electron Transport Complex IV; D002950:Citrate (si)-Synthase; D011345:Fenofibrate