[Biologically active fragment of the differentiation factor from HL-60 cell line. Identification and properties].
Six-membered peptide fragment TGENHR (HLDF-6) was identified in the HL-60 cell culture of human promyelocyte leukemia treated with retinoic acid when studying the differentiation factor HLDF of this cell line. HLDF-6 retains the ability of the full-size factor to induce the differentiation and arrest the proliferation of the starting HL-60 cells. It was shown that the synthetic peptide HLDF-6 has no specific receptors on the surface of the HL-60 cells but can affect the binding of interleukin IL-1 beta, a cytokine involved in proliferation, to the cell surface. It was found on a model of transplantable NSO myeloma that HLDF-6 has an antitumor activity.
Kostanian
I A
IA
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, Russia. kost@ibch.ru
Astapova
M V
MV
Navolotskaia
E V
EV
Lepikhova
T N
TN
Dranitsyna
S M
SM
Telegin
G B
GB
Rodionov
I L
IL
Baĭdakova
L K
LK
Zolotarev
Iu A
IuA
Molotkovskaia
I M
IM
Lipkin
V M
VM
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D002454:Cell Differentiation; D002455:Cell Division / Q000187:drug effects; D018922:HL-60 Cells; D006801:Humans; D016898:Interferon-alpha / Q000378:metabolism; D007375:Interleukin-1 / Q000378:metabolism; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D009363:Neoplasm Proteins / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D011485:Protein Binding; D011994:Recombinant Proteins / Q000378:metabolism; D023041:Xenograft Model Antitumor Assays
D000970:Antineoplastic Agents; D016898:Interferon-alpha; D007375:Interleukin-1; D009363:Neoplasm Proteins; D010446:Peptide Fragments; D011994:Recombinant Proteins; C091149:cell differentiation factor 8.2-kDa