Differential schwann cell migration in adult and old mice: an in vitro study.
The influence of aging on Schwann cell (SC) proliferation, migration and viability was studied in vitro. SCs were cultured in Ham F-10 medium enriched with 20% fetal calf serum (FCS), 40% FCS or collagen I gel plus 20% FCS. The migration of adult mice derived SCs was stimulated with FCS and collagen. With aging, SC migration, multiplication and viability decreased, indicating that ideal culturing conditions should be adjusted.
Santos
A R
AR
Department of Cell Biology, State University of Campinas-UNICAMP, CP 6109, CEP 13089-970, SP, Campinas, Brazil.
Wada
M L
ML
Langone
F
F
Oliveira
A L
AL
D000367:Age Factors; D000375:Aging / Q000502:physiology; D000818:Animals; D001798:Blood Proteins / Q000494:pharmacology; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology; D003470:Culture Media / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012583:Schwann Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D012584:Sciatic Nerve / Q000166:cytology / Q000187:drug effects / Q000502:physiology*
D001798:Blood Proteins; D003470:Culture Media; D003094:Collagen