Exogenous interleukin-10 increased in vitro rejection of allogeneic keratinocytes.
Interleukin-10 (IL-10) is an anti-inflammatory cytokine that has been shown to alter HLA expression in some hemopoietic cells. In skin, the presenting capacity of Langerhans' cells is reduced by IL-10, but little is known about the effect of IL-10 on keratinocytes. Using in vitro experiments, we have shown that IL-10 augments the HLA-DR expression of keratinocytes in the presence of interferon gamma. The increase in HLA-DR expression increased the stimulation and proliferation of allogeneic peripheral blood mononuclear cells, which in turn increased the in vitro rejection of allogeneic keratinocytes. Therefore exogenous application of IL-10 to cultured keratinocytes augments the rejection by allogeneic peripheral blood mononuclear cells in vitro conditions rather than having the suspected protective effect.
Ho-Asjoe
M
M
Centre for Cutaneous Research, Royal London Hospital Medical School, England.
Frame
J D
JD
Leigh
I M
IM
D002056:Burns / Q000276:immunology / Q000628:therapy; D002478:Cells, Cultured; D006084:Graft Rejection / Q000276:immunology*; D006684:HLA-DR Antigens / Q000096:biosynthesis; D006801:Humans; D016753:Interleukin-10 / Q000494:pharmacology*; D015603:Keratinocytes / Q000276:immunology* / Q000637:transplantation; D009000:Monocytes / Q000276:immunology; D014184:Transplantation, Homologous
D006684:HLA-DR Antigens; D016753:Interleukin-10