Use of amplified fragment length polymorphisms for typing Corynebacterium diphtheriae.
Amplified fragment length polymorphism (AFLP) was investigated for the differentiation of Corynebacterium diphtheriae isolates. Analysis using Taxotron revealed 10 distinct AFLP profiles among 57 isolates. Strains with ribotype patterns D1, D4, and D12 could not be distinguished; however, the technique discriminated isolates of ribotype patterns D3, D6, and D7 further. AFLP was rapid, fairly inexpensive, and reproducible and could be used as an alternative to ribotyping.
De Zoysa
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Respiratory and Systemic Infection Laboratory, Central Public Health Laboratory, London NW9 5HT, United Kingdom. adezoysa@phls.org.uk
Efstratiou
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D003353:Corynebacterium diphtheriae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005061:Europe, Eastern; D006801:Humans; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D015183:Restriction Mapping; D021521:Ribotyping / Q000379:methods*