Identification of a novel platelet-derived growth factor-like gene, fallotein, in the human reproductive tract.
We isolated the cDNA of a novel platelet-derived growth factor-like gene from human endometrium. The gene was named fallotein; it was 3007 bases in length, and encoded a protein of 345 amino acids. Antiserum against the fallotein protein can recognize a specific protein in the fallopian tube, with a molecular size in accordance with the anticipated size of fallotein. Fallotein mRNA is expressed in two molecular sizes, 3.8 and 2.9 kb, with the former being more abundant. High expression of the gene was found in the prostate, testis, and uterus. A weaker expression signal was found in the spleen, thymus, and small intestine, but expression of fallotein in the colon and peripheral blood leukocytes was negligible.
Tsai
Y J
YJ
Division of Reproduction and Endocrinolgy, Department of Medical Research, Mackay Memorial Hospital, Tamshui, Taiwan. yjtsai@ms1.mmh.org.tw
Lee
R K
RK
Lin
S P
SP
Chen
Y H
YH
D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary / Q000032:analysis; D004717:Endometrium / Q000502:physiology*; D016228:Endothelial Growth Factors / Q000502:physiology; D020224:Expressed Sequence Tags; D005187:Fallopian Tubes / Q000502:physiology*; D005260:Female; D006801:Humans; D008222:Lymphokines / Q000502:physiology; D008969:Molecular Sequence Data; D010982:Platelet-Derived Growth Factor / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D018076:DNA, Complementary; D016228:Endothelial Growth Factors; D008222:Lymphokines; D010982:Platelet-Derived Growth Factor; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; C411423:platelet-derived growth factor C