title: An interactive web-based Pseudomonas aeruginosa genome database: discovery of new genes, pathways and structures.
abstract: Using the complete genome sequence of Pseudomonas: aeruginosa PAO1, sequenced by the Pseudomonas: Genome Project (ftp://ftp.pseudomonas. com/data/pacontigs.121599), a genome database (http://pseudomonas. bit.uq.edu.au/) has been developed containing information on more than 95% of all ORFs in Pseudomonas: aeruginosa. The database is searchable by a variety of means, including gene name, position, keyword, sequence similarity and Pfam domain. Automated and manual annotation, nucleotide and peptide sequences, Pfam and SMART domains (where available), Medline and GenBank links and a scrollable, graphical representation of the surrounding genomic landscape are available for each ORF. Using the database has revealed, among other things, that P. aeruginosa contains four chemotaxis systems, two novel general secretion pathways, at least three loci encoding F17-like thin fimbriae, six novel filamentous haemagglutinin-like genes, a number of unusual composite genetic loci related to vgr/RHS: elements in Escherichia coli, a number of fix-like genes encoding a micro-oxic respiration system, novel biosynthetic pathways and 38 genes containing domains of unknown function (DUF1/DUF2). It is anticipated that this database will be a useful bioinformatic tool for the Pseudomonas: community that will continue to evolve.
authors.affiliation: Centre for Functional and Applied Genomics, Institute for Molecular Bioscience1 and Department of Biochemistry2, University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: Larry
authors.initials: L
authors.lastname: Croft
authors.affiliation: Centre for Functional and Applied Genomics, Institute for Molecular Bioscience1 and Department of Biochemistry2, University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: Scott A
authors.initials: SA
authors.lastname: Beatson
authors.affiliation: Centre for Functional and Applied Genomics, Institute for Molecular Bioscience1 and Department of Biochemistry2, University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: Cynthia B
authors.initials: CB
authors.lastname: Whitchurch
authors.affiliation: Centre for Functional and Applied Genomics, Institute for Molecular Bioscience1 and Department of Biochemistry2, University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: Bixing
authors.initials: B
authors.lastname: Huang
authors.affiliation: Centre for Functional and Applied Genomics, Institute for Molecular Bioscience1 and Department of Biochemistry2, University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: Robert L
authors.initials: RL
authors.lastname: Blakeley
authors.affiliation: Centre for Functional and Applied Genomics, Institute for Molecular Bioscience1 and Department of Biochemistry2, University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: John S
authors.initials: JS
authors.lastname: Mattick
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002633:Chemotaxis / Q000235:genetics; D016208:Databases, Factual; D010861:Fimbriae, Bacterial / Q000235:genetics / Q000378:metabolism; D016680:Genome, Bacterial; D006388:Hemagglutinins / Q000235:genetics / Q000378:metabolism; D020407:Internet; D008969:Molecular Sequence Data; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D006388:Hemagglutinins