title: Cytokine, elastase and oxygen radical release by Fusobacterium nucleatum-activated leukocytes: a possible pathogenic factor in periodontitis.
abstract: Periodontitis is characterised by tissue destruction caused by reactive oxygen species (ROS) and proteolytic enzymes, which are released by the interaction between bacteria and phagocytes. We estimated the ability of Fusobacterium species to induce release of tissue destructive and proinflammatory mediators from in vitro activated peripheral leukocytes. ROS was measured with the nitroblue tetrazolium (NBT) method, elastase with a specific chromogenic substrate and cytokines, including interleukin 1beta (IL-1beta), tumour necrosis factor alpha (TNF-alpha), and interleukin 8 (IL-8) with a sandwich ELISA method. Various clinical isolates of unopsonized Fusobacterium species stimulated the neutrophils to an increased NBT- reduction. IL-1beta, TNFalpha, IL-8 and elastase were released in significantly higher levels from neutrophils stimulated by Fusobacterium species. In conclusion, unopsonized Fusobacterium species can induce increased production of oxygen radicals, cytokines and elastase from leukocytes activated in vitro.
authors.affiliation: Division of Clinical and Oral Bacteriology, Karolinska Institute, Huddinge Hospital, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Sheikhi
authors.forename: A
authors.initials: A
authors.lastname: Gustafsson
authors.forename: C
authors.initials: C
authors.lastname: Jarstrand
mesh_terms: D000704:Analysis of Variance; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis*; D016967:Fusobacterium nucleatum / Q000472:pathogenicity*; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis; D016209:Interleukin-8 / Q000096:biosynthesis; D019272:Leukocyte Elastase / Q000096:biosynthesis*; D018375:Neutrophil Activation; D009504:Neutrophils / Q000378:metabolism / Q000382:microbiology*; D010518:Periodontitis / Q000209:etiology* / Q000382:microbiology; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D016207:Cytokines; D007375:Interleukin-1; D016209:Interleukin-8; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; D019272:Leukocyte Elastase