N-acetyl L-cysteine attenuates oxidant-mediated toxicity induced by chrysotile fibers.
Chrysotile, an important commercial variety of asbestos, is known to cause oxidative stress by enhancing production of hydrogen peroxide (H(2)O(2)) and thiobarbituric acid reactive substances (TBARS), depleting glutathione (GSH) and altering levels of GSH redox system enzymes. N-acetyl L-cysteine (NAC), a compound that increases GSH levels, protects cells against chrysotile toxicity. In the present study, rats were exposed intratracheally to a single dose (5 mg/rat) of chrysotile. This was followed by a daily dose of NAC 50 mg/kg. b. wt., i.p. At 1, 4, 8 and 16 days post chrysotile exposure lung lavage fluid was collected to determine H(2)O(2) generation, TBARS production, GSH level and its redox system enzymes activities. A significant decrease in H(2)O(2) and TBARS, an increase in GSH content and its redox system enzymes was observed in chrysotile+NAC animals in comparison to chrysotile-exposed animals. In this preliminary study it appears that NAC may be protecting cells against oxidative damage. This protection may be due to its ability to maintain intracellular GSH/oxidative scavenging capability.
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Industrial Toxicology Research Centre, Post Box No. 80, M.G. Marg, 226001, Lucknow, India. rxa48@po.cwru.edu
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D000111:Acetylcysteine / Q000494:pharmacology*; D000818:Animals; D017632:Asbestos, Serpentine / Q000633:toxicity*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002452:Cell Count; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005954:Glucosephosphate Dehydrogenase / Q000187:drug effects / Q000378:metabolism; D005978:Glutathione / Q000187:drug effects / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000187:drug effects / Q000378:metabolism; D005980:Glutathione Reductase / Q000187:drug effects / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D016676:Macrophages, Alveolar / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D016877:Oxidants / Q000378:metabolism*; D051381:Rats; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D017632:Asbestos, Serpentine; D016166:Free Radical Scavengers; D016877:Oxidants; D017392:Thiobarbituric Acid Reactive Substances; D006861:Hydrogen Peroxide; D005954:Glucosephosphate Dehydrogenase; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005978:Glutathione; D000111:Acetylcysteine