title: Leptin plasma concentrations are dependent on body fat distribution in obese patients.
abstract: OBJECTIVE
  To evaluate whether fat distribution plays a role in determining serum leptin concentrations.
  
  
  METHODS
  One-hundred and forty-seven obese patients, 77 males and 70 females, aged 45.1 +/- 13.2 y (mean +/- s.d.; range 21-73 y), with body mass index (BMI) ranging from 30 to 55 kg/m2 (mean 42.3 +/- 5.9). Ultrasound assessment of the thickness of subcutaneous and preperitoneal fat was carried out and calculation of their ratio as abdominal fat index (AFI), waist-hip ratio (WHR), body composition by bioelectrical impedance to evaluate the percentage of fat mass (FM%) and total amount of fat (FMKg) were also determined. Plasma leptin was measured by radio immuno assay (RIA).
  
  
  RESULTS
  In the whole group of patients, serum leptin concentrations were 37.2 +/- 18.4 ng/ml (range 6-101.3 ng/ml); in spite of BMI values not being significantly different, women had leptin values significantly higher (47.4 +/- 17.4 ng/ml) (P < 0.01) than males (28.1 +/- 15.1 ng/ml), also after correction for fat mass. The mean thickness of abdominal subcutaneous fat was 33.7 +/- 12.9 mm and it was significantly (P < 0.001) higher in female (40.9 +/- 10.6 mm) than in male (27.1 +/- 11.2 mm) patients; preperitoneal thickness was 22.9 +/- 7.1 mm, with significantly (P < 0.05) higher values in males (24.2 +/- 6.8 mm) than in females (21.7 +/- 7.3 mm). Accordingly, AFI (in all patients 0.84 +/- 0.6) was significantly higher in males (1.09 +/- 0.6) than in females (0.56 +/- 0.2). In the overall population, leptin concentrations were directly and significantly related to subcutaneous but not preperitoneal fat; they showed a strong inverse relationship with AFI and WHR. When the results were evaluated dividing the patients according to gender, subcutaneous fat thickness showed a stronger association with leptin levels in males than in females, whereas no association was found with preperitoneal fat thickness. Leptin and AFI values were significantly related only in men. WHR values were not correlated with leptin concentrations in either sex. When fat mass was added to the model, subcutaneous fat thickness, AFI and WHR remained independently associated with leptin concentrations. Age and diabetes did not influence these measures.
  
  
  CONCLUSIONS
  Fat distribution contributes to the variability in serum leptin in obese patients. In particular, subcutaneous abdominal fat is a determinant of leptin concentration, also independently of the amount of fat mass, whereas the contribution of preperitoneal visceral fat is not significant.
authors.affiliation: Division of Endocrinology and Metabolic Diseases, Ospedale San Giuseppe IRCCS, Istituto Auxologico Italiano, Verbania, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Minocci
authors.forename: G
authors.initials: G
authors.lastname: Savia
authors.forename: R
authors.initials: R
authors.lastname: Lucantoni
authors.forename: M E
authors.initials: ME
authors.lastname: Berselli
authors.forename: M
authors.initials: M
authors.lastname: Tagliaferri
authors.forename: G
authors.initials: G
authors.lastname: Calò
authors.forename: M L
authors.initials: ML
authors.lastname: Petroni
authors.forename: C
authors.initials: C
authors.lastname: de Medici
authors.forename: G C
authors.initials: GC
authors.lastname: Viberti
authors.forename: A
authors.initials: A
authors.lastname: Liuzzi
mesh_terms: D000005:Abdomen; D000273:Adipose Tissue / Q000000981:diagnostic imaging / Q000378:metabolism*; D000328:Adult; D000368:Aged; D001823:Body Composition; D001824:Body Constitution; D015992:Body Mass Index; D017097:Electric Impedance; D005260:Female; D006801:Humans; D020738:Leptin / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000097:blood / Q000000981:diagnostic imaging / Q000378:metabolism*; D014463:Ultrasonography
chemical_list: D020738:Leptin