Engineering of heterologous cytochrome P450 in Acinetobacter sp.: application for pollutant degradation.
Many organisms do not contain the necessary biochemical armoury to carry out the initial oxidative attack of many pollutant chemicals. In the present study, Acinetobacter sp. strain BD413 has been genetically engineered to express the cytochrome P450 xenobiotic-metabolising enzyme CYP105D1 from Streptomyces griseus that has in its repertoire a diverse array of organic pollutants. Further, it is shown that the transformed Acinetobacter calcoaceticus strain BD413 can grow on pollutants unlike control bacteria not expressing cytochrome P450 and that was reflected in release of radiolabel with growth on radiolabelled chlortoluron. We show that cytochrome P450 can enhance the biodegrading repertoire of A. calcoaceticus and discuss the application of such results to bioremediation strategies.
Lamb
D C
DC
Institute of Biological Sciences, Aberystwyth, SY23 3DA, United Kingdom.
Kelly
D E
DE
Masaphy
S
S
Jones
G L
GL
Kelly
S L
SL
D016954:Acinetobacter calcoaceticus / Q000201:enzymology / Q000235:genetics*; D001426:Bacterial Proteins; D001673:Biodegradation, Environmental; D004785:Environmental Pollutants / Q000378:metabolism*; D005818:Genetic Engineering; D006540:Herbicides / Q000378:metabolism; D010084:Oxidation-Reduction; D010105:Oxygenases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010671:Phenylurea Compounds / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry; D013057:Spectrum Analysis; D013305:Streptomyces griseus / Q000201:enzymology / Q000235:genetics; D014162:Transfection
D001426:Bacterial Proteins; D004785:Environmental Pollutants; D006540:Herbicides; D010671:Phenylurea Compounds; D011994:Recombinant Proteins; D010105:Oxygenases; C122191:CYP105D1 protein, Streptomyces griseus; C007033:chlortoluron