title: A new class of microtubule-associated proteins in plants.
abstract: In plants there are three microtubule arrays involved in cellular morphogenesis that have no equivalent in animal cells. In animals, microtubules are decorated by another class of proteins - the structural MAPS - which serve to stabilize microtubules and assist in their organization. The best-studied members of this class in plants are the MAP-65 proteins that can be purified together with plant microtubules after several cycles of polymerization and depolymerization. Here we identify three similar MAP-65 complementary DNAs representing a small gene family named NtMAP65-1, which encode a new set of proteins, collectively called NtMAP65-1. We show that NtMAP65-1 protein localizes to areas of overlapping microtubules, indicating that it may function in the behaviour of antiparallel microtubules in the mitotic spindle and the cytokinetic phragmoplast.
authors.affiliation: School of Biological Sciences, Royal Holloway University of London, Egham, Surrey, TW20 OEX, UK.
authors.forename: A
authors.initials: A
authors.lastname: Smertenko
authors.forename: N
authors.initials: N
authors.lastname: Saleh
authors.forename: H
authors.initials: H
authors.lastname: Igarashi
authors.forename: H
authors.initials: H
authors.lastname: Mori
authors.forename: I
authors.initials: I
authors.lastname: Hauser-Hahn
authors.forename: C J
authors.initials: CJ
authors.lastname: Jiang
authors.forename: S
authors.initials: S
authors.lastname: Sonobe
authors.forename: C W
authors.initials: CW
authors.lastname: Lloyd
authors.forename: P J
authors.initials: PJ
authors.lastname: Hussey
mesh_terms: D000595:Amino Acid Sequence; D002451:Cell Compartmentation; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D005455:Fluorescent Antibody Technique; D008869:Microtubule-Associated Proteins / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics* / Q000302:isolation & purification; D010947:Plants, Toxic; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D008869:Microtubule-Associated Proteins; D010940:Plant Proteins