title: Antagonistic effect of EGF against TGF beta1 on transformed phenotype and tropomyosin expression of human lung carcinoma A549 cells.
abstract: TGF beta1 suppressed the colony forming ability of human lung carcinoma cell line, A549 cells in a dose-dependent manner. Accompanying these changes, the levels of the expression of high-molecular weight tropomyosin (TM) isoforms, especially TM1 and TM2, were significantly increased in the TGF beta1-treated A549 cells in a dose-dependent manner. When A549 cells were treated with TGF beta1 (2.5 pM) and EGF (1 nM) simultaneously, anchorage-independent growth of A549 cells was restored. In these cells, the expression of high-molecular weight TM decreased. The results suggest that the levels of the expression of high-molecular weight TM may be involved in antagonistic effects of EGF and TGF beta1 on the anchorage-independent growth of A549 cells.
authors.affiliation: Chiba City Health Center, Mihama-ku, Chiba 261-8755, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Tada
authors.forename: H
authors.initials: H
authors.lastname: Kato
authors.forename: S
authors.initials: S
authors.lastname: Hasegawa
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology*; D014411:Neoplastic Stem Cells / Q000187:drug effects; D010641:Phenotype; D020033:Protein Isoforms; D016212:Transforming Growth Factor beta / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D053773:Transforming Growth Factor beta1; D014335:Tropomyosin / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D020033:Protein Isoforms; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D014335:Tropomyosin; D004815:Epidermal Growth Factor