Cleavage of lamin-like proteins in in vivo and in vitro apoptosis of tobacco protoplasts induced by heat shock.
Apoptosis in heat shock-treated tobacco protoplasts was evidenced by DNA fragmentation, flow cytometric analysis and activation of caspase 3-like protease. Furthermore, an in vitro apoptosis system was established which reproduced the apoptotic events. Western blotting analysis using an antibody against lamin A and C showed that in both in vivo and in vitro systems lamin-like proteins were cleaved into a 35-kDa fragment, and that lamin-like protein degradation precedes DNA fragmentation. Moreover, we found a 22.8-fold increase in caspase 6-like activity in cytosol of heat-treated protoplasts as compared with the control.
Chen
H M
HM
Department of Biology, Tsinghua University, Beijing, PR China.
Zhou
J
J
Dai
Y R
YR
D017209:Apoptosis; D053178:Caspase 6; D020169:Caspases / Q000378:metabolism; D002474:Cell-Free System; D018869:Heat-Shock Response; D034904:Lamin Type A; D034882:Lamins; D009687:Nuclear Proteins / Q000378:metabolism*; D010947:Plants, Toxic; D011523:Protoplasts; D014026:Nicotiana
D034904:Lamin Type A; D034882:Lamins; D009687:Nuclear Proteins; D053178:Caspase 6; D020169:Caspases