Histone deacetylase inhibitors and cell proliferation in pea root meristems.
The histone deacetylase (HDA) inhibitors, trichostatin A (TSA) and HC toxin halt mitosis in cultured root meristems of Pisnum sativum, while the anti-protozoan HDA inhibitor apicidin is ineffective. Two-dimensional PAGE of proteins from root meristems exposed to TSA and HC toxin did not show significant differences compared to controls, although a previously tested HDA inhibitor, butyrate, exhibited dramatic variations in its protein profile. Northern analysis of butyrate- and TSA-treated root meristems indicated that non-proliferating cells are expressing significant amounts of transcripts of the known cell proliferation associated genes: histone H2A, MAP kinase, cycA2:1 and cdc2. Western analysis reveals the presence of hyperacetylated nuclear proteins in HDA-inhibitor treated cells. These results suggest that the HDA inhibitors, butyrate and TSA, halt mitosis without down-regulating genes that typically have low or nonexistent expression levels in non-dividing cells.
Murphy
J P
JP
Laboratory of Plant Morphogenesis, Manhattan College, Riverdale, NY 10471, USA.
McAleer
J P
JP
Uglialoro
A
A
Papile
J
J
Weniger
J
J
Bethelmie
F
F
Tramontano
W A
WA
D002455:Cell Division / Q000187:drug effects*; D015180:Electrophoresis, Gel, Two-Dimensional; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D056572:Histone Deacetylase Inhibitors; D018532:Pisum sativum / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D018517:Plant Roots / Q000166:cytology / Q000187:drug effects / Q000201:enzymology
D004791:Enzyme Inhibitors; D056572:Histone Deacetylase Inhibitors