title: [Analytical biotechnology of recombinant peptides and proteins. II. Primary structure of the fusion protein containing human proinsulin and optimization of its proteolysis by trypsin].
abstract: The kinetics of trypsin proteolysis of the fusion protein (FP) containing human proinsulin was studied by a set of analytical micromethods. These were the microcolumn reversed-phase HPLC and the qualitative identification by MALDI-TOF mass spectrometry and amino acid sequencing. The first stage of the proteolysis was shown to be the cleavage of FP into the leader fragment and proinsulin. The subsequent splitting off of C-peptide from proinsulin results in the formation of ArgB31-ArgB32-insulin. The effect of temperature on the formation of de-ThrB30-insulin, a by-product, was also studied. The structure of FP was confirmed by the peptide mapping technique, and the leader fragment was shown to contain no N-terminal Met residue.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Sergeev
authors.forename: N S
authors.initials: NS
authors.lastname: Glukhova
authors.forename: I V
authors.initials: IV
authors.lastname: Nazimov
authors.forename: V A
authors.initials: VA
authors.lastname: Guliaev
authors.forename: I A
authors.initials: IA
authors.lastname: Donetskiĭ
authors.forename: A I
authors.initials: AI
authors.lastname: Miroshnikov
mesh_terms: D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006868:Hydrolysis; D007700:Kinetics; D008969:Molecular Sequence Data; D011384:Proinsulin / Q000737:chemistry*; D011993:Recombinant Fusion Proteins / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013696:Temperature; D014357:Trypsin
chemical_list: D011993:Recombinant Fusion Proteins; D011384:Proinsulin; D014357:Trypsin