Transport of mitochondria during axonogenesis.
The cellular mechanisms involved in axonogenesis are still unclear. In the present work we found that formation of neurites in cultured neonatal dorsal root ganglion neurons co-incided with the redistribution of highly charged mitochondria. Radially distributed in subplasmalemmal space 3 h after plating, highly charged mitochondria formed clusters in the hillocks of predominant neurites during the next 2448 h and then redistributed into the axons. These results provide evidence that accumulation of a critical mass of charged mitochondria at the site of the future axonal hillock may represent the slow initiation stage of axonogenesis, followed by a fast growth phase.
Dedov
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VN
Department of Pharmacy, School of Biological Sciences, University of Sydney, NSW, Australia. vadimd@pharm.usyd.edu.au
Dedova
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IV
Armati
P J
PJ
D000818:Animals; D001369:Axons / Q000502:physiology* / Q000648:ultrastructure; D001693:Biological Transport, Active; D002478:Cells, Cultured; D005727:Ganglia, Spinal / Q000166:cytology; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000502:physiology* / Q000648:ultrastructure; D009068:Movement; D016501:Neurites / Q000502:physiology / Q000648:ultrastructure; D009474:Neurons / Q000502:physiology / Q000648:ultrastructure; D051381:Rats