Single strand conformation polymorphism analysis of K-ras gene mutations by capillary electrophoresis with laser-induced fluorescence (LIF) detector.
Mutations of K-ras gene play an important role in neoplastic progression. The capillary electrophoresis-single strand conformation polymorphism (CE-SSCP) technique is available for the detection of gene mutations. Using an automated capillary electrophoresis with short-chain linear polyacrylamide, after denaturation of PCR products, injections were performed at reverse polarity of 5 kV for 15 s and the separations were carried out under a constant voltage of 8 kV. Of 16 specimens of lung cancer tissue, two specimens were found to have abnormal peaks in the electrophoretogram. CE-SSCP is rapid, automated, and has high performance.
Zhang
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Department of Pharmacy, Henan Medical University, Zhengzhou, 450052, China. zzzhyd@371.net
Wu
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Cheng
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Wu
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D019075:Electrophoresis, Capillary / Q000379:methods*; D011905:Genes, ras; D006801:Humans; D007834:Lasers; D008175:Lung Neoplasms / Q000235:genetics*; D009154:Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D013050:Spectrometry, Fluorescence / Q000379:methods*