Antiviral substance from silkworm faeces: characterization of its antiviral activity.
The antiviral activity of a substance (L4-1) purified from silkworm faeces was examined in an HVJ (Sendai virus)-LLC-MK2 cell system. Its antiviral effect depended on the period of light irradiation and was inhibited by sodium sulfite and anaerobic conditions. These results indicate that the antiviral activity of L4-1 is associated with active oxygen species produced from the substance. SDS-polyacrylamide gel electrophoretic analysis showed that viral proteins were damaged by this substance under light irradiation. The results suggest that the antiviral activity is due to damage to viral protein(s) caused by active oxygen species produced from L4-1.
Hiraki
A
A
Institute of Molecular and Cellular Biology for Pharmaceutical Sciences, Kyoto Pharmaceutical University, Japan.
Hirayama
E
E
Kim
J
J
D000818:Animals; D000998:Antiviral Agents / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000494:pharmacology*; D012831:Bombyx / Q000737:chemistry*; D002460:Cell Line; D002734:Chlorophyll / Q000378:metabolism; D005243:Feces / Q000737:chemistry; D000882:Haplorhini; D008027:Light; D017382:Reactive Oxygen Species / Q000378:metabolism; D018108:Respirovirus / Q000187:drug effects*; D013447:Sulfites / Q000494:pharmacology; D014764:Viral Proteins / Q000187:drug effects
D000998:Antiviral Agents; D017382:Reactive Oxygen Species; D013447:Sulfites; D014764:Viral Proteins; D002734:Chlorophyll; C025026:sodium sulfite