Induction of apoptosis in human leukaemic cell lines K562, HL60 and U937 by diethylhexylphthalate isolated from Aloe vera Linne.
We investigated the effect of diethylhexylphthalate (DEHP) from Aloe vera Linne on the apoptosis of human leukaemic cell lines K562, HL60 and U937 to examine its pharmacological activity. At a level of 10 microg mL(-1) DEHP a significant anti-leukaemic effect was observed for all three cell lines, as measured by clonogenic assay. After treatment with 10 microg mL(-1) DEHP for 4 h, agarose gel electrophoresis and flow cytometric analysis confirmed the occurrence of apoptosis. These results indicate that DEHP isolated from Aloe vera Linne has a potent antileukaemic effect, and thus represents a new type of pharmacological activity with respect to human leukaemic cells.
Lee
K H
KH
Animal Resource Research Center, Konkuk University, Seoul, Korea.
Hong
H S
HS
Lee
C H
CH
Kim
C H
CH
D000504:Aloe; D017209:Apoptosis / Q000187:drug effects*; D004051:Diethylhexyl Phthalate / Q000302:isolation & purification / Q000494:pharmacology*; D004587:Electrophoresis, Agar Gel; D005434:Flow Cytometry; D018922:HL-60 Cells / Q000187:drug effects*; D006801:Humans; D020014:K562 Cells / Q000187:drug effects*; D010946:Plants, Medicinal; D014407:Tumor Cells, Cultured; D014410:Tumor Stem Cell Assay; D020298:U937 Cells / Q000187:drug effects*
D004051:Diethylhexyl Phthalate