An immunoglobulin G based chimeric protein induced foot-and-mouth disease specific immune response in swine.
Epitopes containing the residues 141aa-160aa and 200aa-213aa from foot-and-mouth disease (FMD) serotype O1K HK type FMDV VP1 were joined to a swine immunoglobulin G single heavy chain constant region (scIgG), creating a novel chimeric protein, named F1-scIgG. In this study, inoculation with F1-scIgG induced both FMD virus-neutralizing antibody response and T cell response in swine. Antisera from these F1-scIgG-inoculated swine protected suckling mice against 1000 lethal dose 50 (1000LD(50)) FMD challenge. F1-scIgG-inoculated swine were also fully protected against 50LD(50) FMD virus challenge. The present study demonstrates the clear potential for viral epitopes linked with self-Ig in novel FMD vaccine design.
Chan
E W
EW
Department of Biology, Hong Kong University of Science and Technology, Clearwater Bay, Kowloon, Hong Kong.
Wong
H T
HT
Cheng
S C
SC
Yan
W Y
WY
Zheng
Z X
ZX
Sheng
Z T
ZT
Zhu
L Q
LQ
Xie
Y
Y
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000956:Antigens, Viral / Q000737:chemistry / Q000235:genetics; D005537:Aphthovirus / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D000939:Epitopes / Q000737:chemistry / Q000235:genetics; D005536:Foot-and-Mouth Disease / Q000517:prevention & control; D007074:Immunoglobulin G / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D051379:Mice; D009500:Neutralization Tests; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D013552:Swine; D013601:T-Lymphocytes / Q000276:immunology; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology / Q000494:pharmacology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D017931:DNA Primers; D000939:Epitopes; D007074:Immunoglobulin G; D011993:Recombinant Fusion Proteins; D014765:Viral Vaccines