[Variability of the level of thermoresistance as an indicator of the functional status of a cell].
Effect of acclimating temperature on the thermoresistance of isolated ciliated gill epithelium of Anodonta anatina was studied. One strip of gills from each mussel was used to determine the initial level of thermoresistance (survival time at 40 degrees C). Other strips were kept at 24 degrees C until their death, and their thermoresistance and fluorescence after Ethidium bromide staining were determined at different time intervals. After a short exposure at 24 degrees C the mean level of thermoresistance of the epithelium increased. The individual shift in the value and direction of cell thermoresistance in different mussels negatively correlated with its initial level. It resulted in narrowing the extent of individual variability of the level of thermoresistance. The prolongation of exposure at 24 degrees C decreased the mean level of thermoresistance, disturbed the negative correlation, and increased the variability of thermoresistance level. The value of cell fluorescence remained constant within 48 h. The increase in fluorescence was observed 12 h after reducing the mean thermoresistance level. Hence, the dynamics of variability in the thermoresistance level is a most sensitive indication of the cell functional state.
Mironova
A P
AP
Institute of Cytology RAS, St. Petersburg.
Rozanov
Iu M
IuM
D000818:Animals; D004996:Ethidium; D005453:Fluorescence; D005456:Fluorescent Dyes; D005880:Gills / Q000166:cytology*; D008974:Mollusca / Q000166:cytology; D013696:Temperature
D005456:Fluorescent Dyes; D004996:Ethidium