Distribution of a specific 500-base-pair fragment in mycobacterium bovis isolates from Sardinian cattle.
Amplification of a specific, 500-bp fragment from Mycobacterium bovis isolates and use of the fragment to differentiate between Mycobacterium tuberculosis and M. bovis was previously reported (J. G. Rodriguez, G. A. Meja, P. Del Portillo, M. E. Patarroyo, and L. A. Murillo, Microbiology 141:2131-2138, 1995). In the present study, 30 M. bovis isolates from Sardinian cattle were examined for the presence of this 500-bp fragment; 4 of the 30 isolates lacked the fragment. This result indicates that identification of M. bovis strains by amplification of the 500-bp sequence may lead to false-negative results.
Sechi
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Dipartimento di Scienze Biomediche, Sezione di Microbiologia Sperimentale e Clinica, Università degli studi di Sassari, 07100 Sassari, Italy. sechila@ssmain.uniss.it
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Fadda
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Zanetti
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D000818:Animals; D020029:Base Pairing; D002417:Cattle / Q000382:microbiology*; D004587:Electrophoresis, Agar Gel; D005188:False Negative Reactions; D005784:Gene Amplification; D007558:Italy; D009163:Mycobacterium bovis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics; D015203:Reproducibility of Results; D015183:Restriction Mapping