title: Composite autogenous bone and demineralized bone matrices used to repair defects in the parietal bone of rabbits.
abstract: We compared the amount of new bone produced by endochondral and intramembranous autogenous bone grafts in the presence of demineralized bone matrices (DBMs) prepared from intramembranous bone (DBM(IM)) or endochondral bone (DBM(EC)). Thirty-five bone defects were created in the parietal bone of 20 New Zealand White rabbits. In the experimental groups, 5 defects were grafted with endochondral bone, 5 with endochondral bone mixed with DBM(IM)) (EC-DBM(IM)), 5 with intramembranous bone mixed with DBM(IM)(IM-DBM(IM)) and 6 with endochondral bone mixed with DBM(EC)(EC-DBM(EC)). In the control groups, 10 defects were left alone (passive control) and 4 were grafted with rabbit skin collagen (active control). They were all killed on day 14 and the defects were prepared for histological study. Serial sections were cut across the whole defect. Quantitative analyses were made on 202 sections of the experimental groups by image analysis. A total of 414%, 708%, and 85% more new bone was formed in defects grafted with composite EC-DBM(IM), IM-DBM(IM)and EC-DBM(EC), respectively, than those grafted with endochondral bone alone (P<0.001). No bone was formed in either passive or active controls. In conclusion, demineralized bone matrices, particularly those derived from intramembranous bone, have extremely high osteoinductive properties and greatly improve the integration of autogenous bone grafts in the skull.
authors.affiliation: Orthodontics, Faculty of Dentistry, University of Hong Kong, Prince Philip Hospital, Hong Kong.
authors.forename: A B
authors.initials: AB
authors.lastname: Rabie
authors.forename: R W
authors.initials: RW
authors.lastname: Wong
authors.forename: U
authors.initials: U
authors.lastname: Hägg
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D018775:Bone Demineralization Technique; D001857:Bone Matrix / Q000637:transplantation*; D001861:Bone Regeneration; D016025:Bone Transplantation / Q000379:methods* / Q000473:pathology / Q000706:statistics & numerical data; D003094:Collagen / Q000302:isolation & purification / Q000627:therapeutic use; D010294:Parietal Bone / Q000601:surgery*; D011817:Rabbits; D014182:Transplantation, Autologous
chemical_list: D003094:Collagen