Expression of purinergic receptors (ionotropic P2X1-7 and metabotropic P2Y1-11) during myeloid differentiation of HL60 cells.
The expression of human purinergic P2 receptors (P2X1-7 and P2Y1-11) as well as the ecto-enzymes apyrase (CD39) and 5'-nucleotidase (CD73) was investigated on the nucleic acid level during granulocytic and monocytic differentiation of HL60 cells and on peripheral human blood leukocytes. RT-PCR and dot-blot hybridization assays indicated that mRNA transcripts of all analyzed P2 receptors apart from the P2X3 receptor were expressed during myeloid development of HL60 cells, showing a distinct regulation during the course of differentiation. In blood leukocytes, transcripts of P2X5, P2X7 and all P2Y receptors, except for P2Y6, receptor were found. CD39 and CD73 showed a marked upregulation during myeloid maturation. Functional analysis of P2 receptor-mediated intracellular Ca(2+)-increase after stimulation with ATP revealed no change during granulocytic differentiation, but showed a strong attenuation in both potency and efficacy during monocytic development of HL60 cells.
Adrian
K
K
Department of Biochemistry, University of Erlangen, Fahrstr. 17, 91054 Erlangen, Germany.
Bernhard
M K
MK
Breitinger
H G
HG
Ogilvie
A
A
D015720:5'-Nucleotidase / Q000096:biosynthesis / Q000235:genetics; D000251:Adenosine Triphosphatases; D000255:Adenosine Triphosphate / Q000378:metabolism; D015703:Antigens, CD / Q000096:biosynthesis / Q000235:genetics; D001081:Apyrase; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics* / Q000502:physiology; D015870:Gene Expression; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D018922:HL-60 Cells; D006801:Humans; D015151:Immunoblotting; D012333:RNA, Messenger / Q000096:biosynthesis; D018048:Receptors, Purinergic P2 / Q000096:biosynthesis / Q000235:genetics*; D058469:Receptors, Purinergic P2X; D058486:Receptors, Purinergic P2X7; D058472:Receptors, Purinergic P2Y1
D015703:Antigens, CD; C546468:P2RX7 protein, human; C546382:P2RY1 protein, human; C109734:P2RY11 protein, human; D012333:RNA, Messenger; D018048:Receptors, Purinergic P2; D058469:Receptors, Purinergic P2X; D058486:Receptors, Purinergic P2X7; D058472:Receptors, Purinergic P2Y1; D000255:Adenosine Triphosphate; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D015720:5'-Nucleotidase; D000251:Adenosine Triphosphatases; D001081:Apyrase; C067869:CD39 antigen; D002118:Calcium