title: Glucose-sensitive conductances in rat pancreatic beta-cells: contribution to electrical activity.
abstract: The perforated patch technique was used to assess the relative contribution of K(ATP) channel activity, assessed from input conductance (G(input)), and volume-sensitive anion channel activity to the induction of electrical activity in single isolated rat pancreatic beta-cells by glucose, 2-ketoisocaproate and tolbutamide. In cells equilibrated in the absence of glucose, the membrane potential was -71 mV and G(input) 3.66 nS. Addition of 8 mM glucose resulted in depolarisation, electrical activity and a reduction in G(input), reflecting an inhibition of K(ATP) channels. Cells equilibrated in 4 mM glucose had a membrane potential of -59 mV and a G(input) of 0.88 nS. In this case, a rise in glucose concentration to 8-20 mM again resulted in depolarisation and electrical activity, but caused a small increase in G(input). 2-Ketoisocaproate also evoked electrical activity and an increase in G(input), whereas electrical activity elicited by addition of tolbutamide was accompanied by reduced G(input). Increasing the concentration of glucose from 4 to 8-20 mM generated a noisy inward current at -70 mV, reflecting activation of the volume-sensitive anion channel. The mean amplitude of this current was glucose-dependent within the range 4-20 mM. Addition of 2-ketoisocaproate or a 15% hypotonic solution elicited similar increases in inward current. In contrast, addition of tolbutamide failed to induce the inward current. It is concluded that K(ATP) channel activity is most sensitive to glucose within the range 0-4 mM. At higher glucose concentrations effective in generating electrical activity, activation of the volume-sensitive anion channel could contribute towards the nutrient-induced increase in G(input).
authors.affiliation: Department of Medicine, University of Manchester, Oxford Road, M13 9WL, Manchester, UK. lbest@central.cmht.nwest.nhs.uk
authors.forename: L
authors.initials: L
authors.lastname: Best
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D004553:Electric Conductivity; D005947:Glucose / Q000494:pharmacology*; D066298:In Vitro Techniques; D007515:Islets of Langerhans / Q000737:chemistry / Q000166:cytology / Q000187:drug effects*; D007651:Keto Acids / Q000494:pharmacology; D008564:Membrane Potentials; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000737:chemistry / Q000187:drug effects*; D051381:Rats; D014044:Tolbutamide / Q000494:pharmacology
chemical_list: D007651:Keto Acids; D015221:Potassium Channels; C013082:alpha-ketoisocaproic acid; D014044:Tolbutamide; D005947:Glucose