title: Glycogen granules in resting and inflammatory rainbow trout phagocytes--an ultrastructural study.
abstract: The ultrastructural image of glycogen granules in the cytoplasm of rainbow trout phagocytes in sections stained by the conventional lead or uranyl-lead stains is highly dependent on fixation conditions, the granules being visible only when adequate fixation protocols are used. Morphometry of samples processed for the detection of peroxidase or esterase activities (to specifically label neutrophils and macrophages, respectively), and simultaneously stained for the specific detection of glycogen, showed that inflammatory peritoneal neutrophils were richer in glycogen granules than resting neutrophils. This increase in glycogen content occurs after the migration from the haematopoietic tissues and peripheral blood to the inflamed foci. Glycogen granules could not be found in resting peritoneal macrophages but were found in inflammatory macrophages. The macrophage granules occurred in smaller amounts than in neutrophils, and consisted of granules identical to those of neutrophils together with significantly smaller granules. No evidence for the utilization of glycogen by neutrophils phagocytosing bacteria within the peritoneal cavity was found.
authors.affiliation: Institute for Molecular and Cell Biology, University of Porto, Portugal. aafonso@ibmc.up.pt
authors.forename: A
authors.initials: A
authors.lastname: Afonso
authors.forename: P M
authors.initials: PM
authors.lastname: Macedo
authors.forename: A E
authors.initials: AE
authors.lastname: Ellis
authors.forename: M T
authors.initials: MT
authors.lastname: Silva
mesh_terms: D000818:Animals; D017756:Aquaculture; D001786:Blood Glucose / Q000032:analysis; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D005393:Fish Diseases / Q000097:blood / Q000276:immunology*; D006003:Glycogen / Q000032:analysis*; D007249:Inflammation / Q000097:blood / Q000276:immunology / Q000662:veterinary*; D017737:Macrophages, Peritoneal / Q000737:chemistry / Q000648:ultrastructure; D009504:Neutrophils / Q000737:chemistry / Q000648:ultrastructure; D017686:Oncorhynchus mykiss / Q000097:blood / Q000276:immunology*; D010586:Phagocytes / Q000737:chemistry / Q000648:ultrastructure*; D015007:Yersinia; D015009:Yersinia Infections / Q000276:immunology / Q000662:veterinary
chemical_list: D001786:Blood Glucose; D006003:Glycogen