Growth of Photorhabdus luminescens in batch and glucose fed-batch culture.
Photorhabdus luminescens, a bacterial symbiont of entomopathogenic biocontrol nematodes, was grown in batch and glucose fed-batch culture. The cell density, bioluminescence, production of antibiotic substances, number of cells with inclusion bodies, glucose concentration and oxygen uptake rate were recorded. The addition of 12.4 g 1(-1) glucose prolonged the growth, and the yield almost doubled, from 6.85 g 1(-1) to 12.45 g 1(-1) dry mass. The production of antibiotic substances increased by 140%. Bioluminescence was higher in the batch culture. A shift of P. luminescens to phase II variants was not detected.
Jeffke
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T
Institute for Phytopathology, Department of Biotechnology and Biological Control, Christian-Albrechts-University Kiel, Raisdorf, Germany.
Jende
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D
Mätje
C
C
Ehlers
R U
RU
Berthe-Corti
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L
D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D019149:Bioreactors; D015169:Colony Count, Microbial; D003470:Culture Media; D005947:Glucose / Q000378:metabolism*; D002479:Inclusion Bodies / Q000378:metabolism; D008163:Luminescent Measurements; D010101:Oxygen Consumption; D020637:Photorhabdus / Q000254:growth & development* / Q000378:metabolism
D000900:Anti-Bacterial Agents; D003470:Culture Media; D005947:Glucose