HLA-B*4012: a new allele with unique serological features.
We have defined the new allele HLA-B*4012, which had been isolated from a black individual. It was initially recognized as a serologically unique allele when typing her father for renal transplantation. The HLA class I phenotype was A*0201,*6602; B*4001,*4012; Bw6; Cw*0304,*1505. Sequencing from exon 1 through intron 3 of B*4012 was performed. B*4012 is identical to B*4001 and B*4010 in exon 3, and in the 3' part of exon 2, but it is unique in that exon 1 and the 5' part of exon 2 are identical to B*1503, B*1509, B*1510, B*1518, B*1523, and B*1529. The generation of this allele is best explained by a recombination event in exon 2 (break point between nucleotides 205 and 222 from the beginning of the coding region) of B*4001 or B*4010 with one of these B*15 variants as a donor allele. Its unique serological feature (B48, B60, B70, and B72 reactivity) is consistent with the sequence data of its donor alleles.
Elsner
H A
HA
Department of Transfusion Medicine, Hannover Medical School, Germany.
O'Brien
B J
BJ
Bryan
C F
CF
Meyer
J A
JA
Diederich
D A
DA
Pierce
G E
GE
Lau
M
M
Terasaki
P I
PI
Blasczyk
R
R
D000483:Alleles; D005091:Exons; D005260:Female; D015235:HLA-B Antigens / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D006239:Haplotypes; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology; D008297:Male; D008969:Molecular Sequence Data; D010375:Pedigree; D010802:Phylogeny; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary
D015235:HLA-B Antigens