title: Stimulation of glutamate receptor protein synthesis and membrane insertion within isolated neuronal dendrites.
abstract: The selective subcellular localization of mRNAs to dendrites and the recent demonstration of local protein synthesis have highlighted the potential role of postsynaptic sites in modulation of cell-cell communication. We show that epitope-tagged subunit 2 of the ionotopic glutamate receptor, GluR2, mRNA transfected into isolated hippocampal neuronal dendrites is translated in response to pharmacologic stimulation. Further, confocal imaging of N-terminally labeled GluR2 reveals that the newly synthesized GluR2 protein can integrate into the dendritic membrane with the N terminus externally localized. These data demonstrate that integral membrane proteins can be synthesized in dendrites and can locally integrate into the cell membrane.
authors.affiliation: Departments of Pharmacology and Psychiatry, and Neurology, University of Pennsylvania Medical Center, Philadelphia, PA 19104-6084, USA.
authors.forename: J E
authors.initials: JE
authors.lastname: Kacharmina
authors.forename: C
authors.initials: C
authors.lastname: Job
authors.forename: P
authors.initials: P
authors.lastname: Crino
authors.forename: J
authors.initials: J
authors.lastname: Eberwine
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D003712:Dendrites / Q000378:metabolism*; D018870:Endoplasmic Reticulum, Rough / Q000378:metabolism; D016259:Genes, myc; D006056:Golgi Apparatus / Q000378:metabolism; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D009474:Neurons / Q000378:metabolism*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017470:Receptors, Glutamate / Q000096:biosynthesis* / Q000235:genetics; D014162:Transfection
chemical_list: D017931:DNA Primers; D008565:Membrane Proteins; D012333:RNA, Messenger; D017470:Receptors, Glutamate