Galactosylated chitosan-graft-dextran as hepatocyte-targeting DNA carrier.
Lactobionic acid bearing galactose group was coupled with chitosan for liver specificity, and dextran was grafted to galactosylated chitosan (GC) for stability in water. Compared to the GC/DNA complex, the stability of the galactosylated chitosan-graft-dextran (GCD)/DNA complex could be enhanced. The particle size of the GCD/DNA complexes decreased as the charge ratio of GCD to DNA increased. Conformational change of DNA did not occur after complex formation with GCD compared with the conformation of DNA itself. The GCD/DNA complexes were only transfected into Chang liver cells and that of Hep G2 having asialoglycoprotein receptors (ASGR), indicative of specific interaction of ASGRs on cells and galactose ligands on chitosan.
Park
I K
IK
School of Agricultural Biotechnology, Seoul National University, Suwon 441-744, South Korea.
Park
Y H
YH
Shin
B A
BA
Choi
E S
ES
Kim
Y R
YR
Akaike
T
T
Cho
C S
CS
Park
Y K
YK
Park
Y R
YR
D037263:Asialoglycoprotein Receptor; D002240:Carbohydrate Sequence; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry; D048271:Chitosan; D002942:Circular Dichroism; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics*; D003911:Dextrans; D004187:Disaccharides / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry*; D005690:Galactose / Q000737:chemistry*; D006367:HeLa Cells; D022781:Hepatocytes / Q000187:drug effects*; D006801:Humans; D008027:Light; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D009391:Nephelometry and Turbidimetry; D010957:Plasmids / Q000737:chemistry; D011956:Receptors, Cell Surface / Q000378:metabolism; D012542:Scattering, Radiation; D014162:Transfection
D037263:Asialoglycoprotein Receptor; D003911:Dextrans; D004187:Disaccharides; D004337:Drug Carriers; D011956:Receptors, Cell Surface; D002686:Chitin; C005608:lactobionic acid; D004247:DNA; D048271:Chitosan; D005690:Galactose