title: Adhesion and growth of CaCo2 cells on surface-modified PEEK substrata.
abstract: A series of surface-functionalized poly(ether ether ketone) (PEEK) films has been prepared by selective wet-chemistry; they are hydroxylated polymer (PEEK-OH) obtained by reduction, aminated polymer (PEEK-[]-NH2) prepared by coupling a diisocyanate reagent to PEEK-OH (PEEK-[]-NCO) followed by hydrolysis, and carboxylated and aminocarboxylated polymers (PEEK-[]-GABA and PEEK-Lysine) resulting from the coupling of aminoacids to PEEK-[]-NCO. The aminated and carboxylated substrata promoted the adhesion and growth of CaCo2 cells in the presence of serum. Fibronectin (FN), an extra-cellular matrix protein, has been covalently fixed and/or adsorbed on various PEEK substrata, in the presence or not of a polymeric surfactant (Pluronic F68). The performances of the FN-grafted substrata (PEEK-[]-FN(1) and PEEK-[]-FN(2)) were significantly higher than those of reference substrata simply coated with FN (PEEK-OH(+FN)(1) and (2), PEEK-[]-NH2(+FN)(1) and (2)), considering the adhesion and spreading of CaCo2 cells in the absence of serum. Moreover, the stability of the adherent cells on the FN-adsorbed substrata dramatically depended on the experimental conditions applied during the PEEK coating with FN.
authors.affiliation: Laboratoire de Chimie Organique de Synthèse, Université catholique de Louvain, Départment de Chimie, Louvain-la-Neuve, Belgium.
authors.forename: O
authors.initials: O
authors.lastname: Noiset
authors.forename: Y J
authors.initials: YJ
authors.lastname: Schneider
authors.forename: J
authors.initials: J
authors.lastname: Marchand-Brynaert
mesh_terms: D001577:Benzophenones; D001672:Biocompatible Materials; D002448:Cell Adhesion / Q000502:physiology*; D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division / Q000502:physiology*; D002460:Cell Line; D006801:Humans; D007659:Ketones; D011092:Polyethylene Glycols / Q000138:chemical synthesis; D011108:Polymers; D013329:Structure-Activity Relationship
chemical_list: D001577:Benzophenones; D001672:Biocompatible Materials; D007659:Ketones; D011108:Polymers; C063834:polyetheretherketone; D011092:Polyethylene Glycols