A new positive/negative selectable marker, puDeltatk, for use in embryonic stem cells.
A novel positive/negative selection cassette, puDeltatk, was generated. pu(Delta)tk is a bifunctional fusion protein between puromycin N-acetyltransferase (Puro) and a truncated version of herpes simplex virus type 1 thymidine kinase (DeltaTk). Murine embryonic stem (ES) cells transfected with pu(Delta)tk become resistant to puromycin and sensitive to 1-(-2-deoxy-2-fluoro-1-beta-D-arabino-furanosyl)-5-iodouracil (FIAU). Unlike other HSV1 tk transgenes, puDeltatk is readily transmitted through the male germ line. Thus pu(Delta)tk is a convenient positive/negative selectable marker that can be widely used in many ES cell applications.
Chen
Y T
YT
Program in Developmental Biology, Baylor College of Medicine, Houston, Texas 77030, USA.
Bradley
A
A
D000123:Acetyltransferases / Q000235:genetics*; D000818:Animals; D019677:Artificial Gene Fusion / Q000379:methods; D002478:Cells, Cultured; D002999:Clone Cells / Q000201:enzymology; D004351:Drug Resistance / Q000235:genetics; D018274:Electroporation; D004622:Embryo, Mammalian / Q000166:cytology* / Q000201:enzymology*; D005819:Genetic Markers; D018259:Herpesvirus 1, Human / Q000201:enzymology / Q000235:genetics; D008297:Male; D051379:Mice; D010735:Phosphoglycerate Kinase / Q000235:genetics; D013234:Stem Cells / Q000201:enzymology*; D013937:Thymidine Kinase / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics
D005819:Genetic Markers; D014764:Viral Proteins; D000123:Acetyltransferases; C045336:puromycin N-acetyltransferase; D013937:Thymidine Kinase; D010735:Phosphoglycerate Kinase