title: CD1d on myeloid dendritic cells stimulates cytokine secretion from and cytolytic activity of V alpha 24J alpha Q T cells: a feedback mechanism for immune regulation.
abstract: The precise immunologic functions of CD1d-restricted, CD161+ AV24AJ18 (Valpha24JalphaQ) T cells are not well defined, although production of IL-4 has been suggested as important for priming Th2 responses. However, activation of human Valpha24JalphaQ T cell clones by anti-CD3 resulted in the secretion of multiple cytokines notably important for the recruitment and differentiation of myeloid dendritic cells. Specific activation of Valpha24JalphaQ T cells was CD1d restricted. Expression of CD1d was found on monocyte-derived dendritic cells in vitro, and immunohistochemical staining directly revealed CD1d preferentially expressed on dendritic cells in the paracortical T cell zones of lymph nodes. Moreover, myeloid dendritic cells both activated Valpha24JalphaQ T cells and were susceptible to lysis by these same regulatory T cells. Because myeloid dendritic cells are a major source of IL-12 and control Th1 cell differentiation, their elimination by lysis is a mechanism for limiting the generation of Th1 cells and thus regulating Th1/Th2 responses.
authors.affiliation: Division of Infectious Diseases, University of California Medical Center, Los Angeles 90095, USA. oyang@mednet.ucla.edu
authors.forename: O O
authors.initials: OO
authors.lastname: Yang
authors.forename: F K
authors.initials: FK
authors.lastname: Racke
authors.forename: P T
authors.initials: PT
authors.lastname: Nguyen
authors.forename: R
authors.initials: R
authors.lastname: Gausling
authors.forename: M E
authors.initials: ME
authors.lastname: Severino
authors.forename: H F
authors.initials: HF
authors.lastname: Horton
authors.forename: M C
authors.initials: MC
authors.lastname: Byrne
authors.forename: J L
authors.initials: JL
authors.lastname: Strominger
authors.forename: S B
authors.initials: SB
authors.lastname: Wilson
mesh_terms: D000911:Antibodies, Monoclonal / Q000494:pharmacology; D018949:Antigens, CD1 / Q000096:biosynthesis / Q000276:immunology*; D002461:Cell Line, Transformed / Q000276:immunology / Q000378:metabolism; D019070:Cell Lineage / Q000276:immunology; D002999:Clone Cells; D016207:Cytokines / Q000378:metabolism*; D003602:Cytotoxicity, Immunologic / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D005246:Feedback; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology; D009000:Monocytes / Q000276:immunology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000096:biosynthesis*; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D018949:Antigens, CD1; D016207:Cytokines; D016693:Receptors, Antigen, T-Cell, alpha-beta