Glucose transporter 1 expression in corneal wound repair under high serum glucose level.
OBJECTIVE
To determine glucose transporter (GLUT) 1 mRNA and protein expression during corneal epithelial wound healing in diabetic rat.


METHODS
Diabetes mellitus was induced by intraperitoneal injection of streptozotocin. At 10 days after injection, unilateral 3-mm epithelial debridement was carried out in the central cornea. At 2, 4, 6, and 24 hours after wounding, whole corneal epithelium was collected and GLUT1 protein and mRNA levels were determined by Western blotting and reverse transcription-polymerase chain reaction, respectively. Sugar content in collected samples was measured by the Anthrone reaction. Normal rats were used as controls.


RESULTS
Glucose transporter 1 protein and mRNA levels in unwounded cornea were similarly low in the diabetic and control groups. Healing of corneal wounds was slower in diabetic rats than in controls. After wounding, GLUT1 mRNA and protein expression in both groups were similarly enhanced compared to unwounded epithelium. Sugar content at all time points did not show significant alteration in any group, although in diabetic rats it was significantly higher than in controls throughout the time course.


CONCLUSIONS
Glucose transporter 1 expression in diabetic rat cornea showed little difference from that in normal rat cornea, suggesting minimal influence of GLUT1 on the delayed healing of diabetic corneal wounds.
Takahashi
H
H
Department of Ophthalmology, Nippon Medical School, Tokyo, Japan.
Ohara
K
K
Ohmura
T
T
Takahashi
R
R
Zieske
J D
JD
D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity; D015415:Biomarkers; D001786:Blood Glucose / Q000378:metabolism*; D015153:Blotting, Western; D003921:Diabetes Mellitus, Experimental / Q000097:blood* / Q000139:chemically induced / Q000150:complications; D019573:Epithelium, Corneal / Q000293:injuries / Q000378:metabolism* / Q000473:pathology; D005131:Eye Injuries / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D051272:Glucose Transporter Type 1; D009004:Monosaccharide Transport Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013311:Streptozocin / Q000633:toxicity; D014945:Wound Healing / Q000502:physiology*
D000900:Anti-Bacterial Agents; D015415:Biomarkers; D001786:Blood Glucose; D051272:Glucose Transporter Type 1; D009004:Monosaccharide Transport Proteins; D012333:RNA, Messenger; C495679:Slc2a1 protein, rat; D013311:Streptozocin