title: Prevalence of human papillomavirus DNA in different histological subtypes of cervical adenocarcinoma.
abstract: The prevalence of human papilloma virus (HPV) DNA in different histological subtypes of cervical adenocarcinoma and related tumors was examined using formalin-fixed, paraffin-embedded tissue samples from 105 primary cervical adenocarcinomas and adenosquamous carcinomas. Broad-spectrum HPV DNA amplification and genotyping was performed with the SPF10 primer set and line probe assay (LiPA), respectively. HPV DNA was detected in 82 of 90 (91%) mucinous adenocarcinomas, encompassing endocervical, intestinal, and endometrioid histological subtypes, and in nine of nine adenosquamous tumors (100%). HPV DNA was not detected in any nonmucinous adenocarcinomas including clear cell, serous, and mesonephric carcinomas (0/6). The most common viral types detected in adenocarcinoma were HPV 16 (50%) and HPV 18 (40%), followed by HPV 45 (10%), HPV52 (2%), and HPV 35 (1%). Multiple HPV types were detected in 9.7% of the cases. In conclusion, mucinous adenocarcinomas and adenosquamous carcinomas of the cervix demonstrate a very high prevalence of HPV DNA, similar to that reported for cervical squamous cell carcinoma. Only rare histological variants of cervical adenocarcinoma seem unrelated to HPV infection.
authors.affiliation: Department of Pathology, Weill Medical College of Cornell University, New York, New York. Lenox Hill Hospital, New York, New York, USA. ecpirog@mail.med.cornell.edu
authors.forename: E C
authors.initials: EC
authors.lastname: Pirog
authors.forename: B
authors.initials: B
authors.lastname: Kleter
authors.forename: S
authors.initials: S
authors.lastname: Olgac
authors.forename: P
authors.initials: P
authors.lastname: Bobkiewicz
authors.forename: J
authors.initials: J
authors.lastname: Lindeman
authors.forename: W G
authors.initials: WG
authors.lastname: Quint
authors.forename: R M
authors.initials: RM
authors.lastname: Richart
authors.forename: C
authors.initials: C
authors.lastname: Isacson
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology* / Q000821:virology*; D002288:Adenocarcinoma, Mucinous / Q000473:pathology / Q000821:virology; D000328:Adult; D018196:Carcinoma, Adenosquamous / Q000473:pathology / Q000821:virology; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics*; D015995:Prevalence; D002583:Uterine Cervical Neoplasms / Q000473:pathology* / Q000821:virology*
chemical_list: D004279:DNA, Viral