Production kinetics of acidophilin 801, a bacteriocin produced by Lactobacillus acidophilus IBB 801.
Lactobacillus acidophilus IBB 801 produces a small bacteriocin, designated acidophilin 801. Studying the relationship between growth and bacteriocin biosynthesis revealed primary metabolite kinetics of bacteriocin production with a peak activity at the end of the exponential growth phase followed by a decrease during the stationary phase. Both microbial growth and bacteriocin production was inhibited by lactic acid. Whereas volumetric bacteriocin production (activity units (AU) ml(-1)) was favoured under pH-controlled conditions, bacteriocin titres rapidly decreased because of strong adsorption of the bacteriocin molecules to the producing cells under less acidic conditions.
Zamfir
M
M
Genetic Engineering Laboratory, Institute of Biology, Bucharest, Romania.
Callewaert
R
R
Cornea
P C
PC
De Vuyst
L
L
D001430:Bacteriocins / Q000096:biosynthesis*; D003470:Culture Media; D007700:Kinetics; D007779:Lactobacillus acidophilus / Q000254:growth & development* / Q000378:metabolism*
D001430:Bacteriocins; D003470:Culture Media; C405711:acidophilin 801