Cloning and promoter analysis of the cotton lipid transfer protein gene Ltp3(1).
A cotton Ltp3 gene and its 5' and 3' flanking regions have been cloned with a PCR-based genomic DNA walking method. The amplified 2.6 kb DNA fragment contains sequences corresponding to GH3 cDNA which has been shown to encode a lipid transfer protein (LTP3). The gene has an intron of 80 bp which is located in the region corresponding to the C-terminus of LTP3. The Ltp3 promoter was systematically analyzed in transgenic tobacco plants by employing the Escherichia coli beta-glucuronidase gene (GUS) as a reporter. The results of histochemical and fluorogenic GUS assays indicate that the 5' flanking region of the Ltp3 gene contains cis-elements conferring the trichome specific activity of Ltp3 promoter.
Liu
H C
HC
Department of Biochemistry and Molecular Biology, Box 9650, Mississippi State University, Mississippi State, MS 39762, USA.
Creech
R G
RG
Jenkins
J N
JN
Ma
D P
DP
D000595:Amino Acid Sequence; D052179:Antigens, Plant; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis / Q000737:chemistry; D017343:Genes, Plant; D003368:Gossypium / Q000235:genetics*; D008969:Molecular Sequence Data; D010940:Plant Proteins; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D013784:Textiles; D014026:Nicotiana / Q000235:genetics
D052179:Antigens, Plant; D002352:Carrier Proteins; D018076:DNA, Complementary; D010940:Plant Proteins; C511920:lipid transfer proteins, plant