A chloroplastic RNA-binding protein is a new member of the PPR family.
P67, a new protein binding to a specific RNA probe, was purified from radish seedlings [Echeverria, M. and Lahmy, S. (1995) Nucleic Acids Res. 23, 4963-4970]. Amino acid sequence information obtained from P67 microsequencing allowed the isolation of genes encoding P67 in radish and Airabidopsis thaliana. Immunolocalisation experiments in transfected protoplasts demonstrated that this protein is addressed to the chloroplast. The RNA-binding activity of recombinant P67 was found to be similar to that of the native protein. A significant similarity with the maize protein CRP1 [Fisk, D.G., Walker, M.B. and Barkan, A. (1999) EMBO J. 18, 2621-2630] suggests that P67 belongs to the PPR family and could be involved in chloroplast RNA processing.
Lahmy
S
S
Laboratoire Génome et Développement des Plantes', Université de Perpignan UMR CNRS 5096, France.
Barnèche
F
F
Derancourt
J
J
Filipowicz
W
W
Delseny
M
M
Echeverria
M
M
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D017360:Arabidopsis; D001483:Base Sequence; D001937:Brassica; D002736:Chloroplasts / Q000378:metabolism*; D003001:Cloning, Molecular; D018744:DNA, Plant; D015870:Gene Expression; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger; D016601:RNA-Binding Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism; D013347:Subcellular Fractions
D018744:DNA, Plant; D010940:Plant Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; C415086:p67 protein, Raphanus sativus