Octadecanoid and jasmonate signaling in tomato (Lycopersicon esculentum Mill.) leaves: endogenous jasmonates do not induce jasmonate biosynthesis.
Jasmonates and their precursors, the octadecanoids, are signals in stress-induced alteration of gene expression. Several mRNAs coding for enzymes of jasmonic acid (JA) biosynthesis are up-regulated upon JA treatment or endogenous increase of the JA level. Here we investigated the positive feedback of endogenous JA on JA formation, as well as its beta-oxidation steps. JA-responsive gene expression was recorded in terms of proteinase inhibitor2 (pin2) mRNA accumulation. JA formed upon treatment of tomato (Lycopersicon esculentum cv. Moneymaker) leaves with JA derivatives carrying different lengths of the carboxylic acid side chain was quantified by gas chromatography-mass spectrometry (GC-MS). The data revealed that beta-oxidation of the side chain occurs up to a butyric acid moiety. The amount of JA formed from side-chain modified JA derivatives correlated with pin2-mRNA accumulation. JA derivatives with a carboxylic side chain of 3, 5 or 7 carbon atoms were unable to form JA and to express on pin2, whereas even-numbered derivatives were active. After treatment of tomato leaves with (10-(2)H)-(-)-12-oxophytoenoic acid, (4-(2)H)-(-)-JA and its methyl ester were formed and could be quantified separately from the endogenously nonlabeled JA pool by GC-MS analysis via isotopic discrimination. The level of 8 nmol per g fresh weight JA and its methyl ester originated exclusively from labeled 12-oxophytoenic acid. This and further data indicate that endogenous synthesis of the JA precursor 12-oxophytodienoic acid, as well as of JA and its methyl ester, are not induced in tomato leaves, suggesting that positive feedback in JA biosynthesis does not function in vivo.
Miersch
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O
Leibniz Institute of Plant Biochemistry, Halle, Germany.
Wasternack
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C
D003517:Cyclopentanes / Q000032:analysis / Q000378:metabolism* / Q000494:pharmacology*; D005246:Feedback / Q000187:drug effects / Q000502:physiology; D008401:Gas Chromatography-Mass Spectrometry; D005786:Gene Expression Regulation / Q000187:drug effects; D018551:Solanum lycopersicum; D010084:Oxidation-Reduction; D054883:Oxylipins; D010937:Plant Growth Regulators / Q000378:metabolism / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D010940:Plant Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D011480:Protease Inhibitors; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D015398:Signal Transduction; D013229:Stearic Acids / Q000378:metabolism / Q000494:pharmacology
D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D011480:Protease Inhibitors; D012333:RNA, Messenger; D013229:Stearic Acids; C034104:proteinase inhibitor II protein, plant; C098204:12-oxooctadecanoic acid; C031183:stearic acid; C011006:jasmonic acid