title: Development of bioactive food packaging materials using immobilised bacteriocins lacticin 3147 and nisaplin.
abstract: Immobilisation of the bacteriocins nisin and lacticin 3147 to packaging materials was investigated. Stability of both cellulose-based bioactive inserts and anti-microbial polyethylene/polyamide pouches was examined over time. Anti-microbial activity against the indicator strain Lactococcus lactis subsp. lactis HP, in addition to Listeria innocua DPC 1770 and Staphylococcus aureus MMPR3 was observed for all bacteriocin-adsorbed materials. Activity retention of the inserts showed an initial decrease in the first week of storage but remained stable for the remaining 3 months of the trial. However, adsorption of lacticin 3147 to plastic film was unsuccessful, nisin bound well and the resulting film maintained its activity for 3-month period, both at room temperature and under refrigeration. When applied to food systems, the anti-microbial packaging reduced the population of lactic acid bacteria in sliced cheese and ham stored in modified atmosphere packaging (MAP) at refrigeration temperatures, thus extending the shelf life. Nisin-adsorbed bioactive inserts reduced levels of Listeria innocua by > or = 2 log units in both products, and Staphylococcus aureus by approximately 1.5 log units in cheese, and approximately 2.8 log units in ham. Similar reductions were observed in cheese vacuum-packaged in nisin-adsorbed pouches.
authors.affiliation: Department of Food Technology, Universiry College, Cork, Ireland. a.scannell@ucc.ie
authors.forename: A G
authors.initials: AG
authors.lastname: Scannell
authors.forename: C
authors.initials: C
authors.lastname: Hill
authors.forename: R P
authors.initials: RP
authors.lastname: Ross
authors.forename: S
authors.initials: S
authors.lastname: Marx
authors.forename: W
authors.initials: W
authors.lastname: Hartmeier
authors.lastname: Elke
authors.forename: K
authors.initials: K
authors.lastname: Arendt
mesh_terms: D000818:Animals; D001419:Bacteria / Q000187:drug effects* / Q000254:growth & development; D001426:Bacterial Proteins / Q000494:pharmacology*; D001430:Bacteriocins / Q000494:pharmacology*; D002611:Cheese / Q000382:microbiology; D005516:Food Microbiology; D018857:Food Packaging / Q000379:methods*; D005519:Food Preservation; D005520:Food Preservatives / Q000494:pharmacology; D013294:Lactococcus lactis / Q000187:drug effects; D008087:Listeria / Q000187:drug effects; D008461:Meat Products / Q000382:microbiology; D009561:Nisin / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects; D013552:Swine; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D001430:Bacteriocins; D005520:Food Preservatives; C082508:lacticin 481; D009561:Nisin