Binding of catalase by Gardnerella vaginalis.
Previous work has demonstrated that Gardnerella vaginalis can utilize catalase as a sole source of iron. In this study, the interaction between G. vaginalis cells and catalase was investigated. G. vaginalis cells were shown to bind digoxigenin (DIG)-labeled catalase using a solid phase dot blot assay. An increase in catalase binding was observed from cells grown under iron-restrictive conditions. Western blot analysis of G. vaginalis proteins resulted in the detection of a putative catalase-binding protein with an estimated molecular mass of 128 kDa. The 128-kDa catalase-binding protein was not detected from intact G. vaginalis cells treated with trypsin prior to Western blot analysis suggesting this protein may be surface-exposed.
Jarosik
G P
GP
Department of Biological Sciences, Louisiana State University, Baton Rouge 70803, USA. gjarosi@unix1.sncc.lsu.edu
D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D015153:Blotting, Western; D002374:Catalase / Q000378:metabolism*; D003470:Culture Media; D004076:Digoxigenin / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006194:Gardnerella vaginalis / Q000254:growth & development / Q000378:metabolism*; D007501:Iron / Q000378:metabolism
D001426:Bacterial Proteins; D003470:Culture Media; D007501:Iron; D002374:Catalase; D004076:Digoxigenin