Effects of CO2 insufflation and laparotomy on wound healing in mice.
BACKGROUND
The aim of the present study was to evaluate the effects of laparotomy and CO2 insufflation on wound healing in a murine incisional wound healing model.


METHODS
Seventy-two male Swiss Albino mice were randomly allocated into three groups of control, laparotomy and CO2 insufflation. A transverse skin incision of 15 mm was made in the dorsum of each mouse, and four interrupted mattress sutures with 4.0 polypropylene thread were laid for wound closure. A median laparotomy was performed in the laparotomy group. CO2 insufflation was performed with an intra-abdominal pressure of 9 mmHg. The retained gas was evacuated from the abdominal cavity at the end of a 60-min period. Mice were killed on the 3rd, 7th and 15th postoperative days. The wound tensile strength and 5-hydroxyproline concentration in the wound tissue were measured.


RESULTS
Tensile strength of the incised skin increased as the post-incision period progressed. There was no significant difference between the tensile strengths of the incised skin of control, laparotomy and CO2 insufflation groups throughout the observation period. The skin 5-hydroxyproline concentrations of all groups were not significantly different at the 3rd postoperative day. But laparotomy and CO2 insufflation groups had lower 5-hydroxyproline concentrations at the 7th and 15th postoperative days, when compared to controls (P < 0.02 for 7th and 15th days).


CONCLUSIONS
CO2 insufflation and laparotomy reduce the 5-hydroxyproline concentration of the wound, suggesting a diminished wound healing capacity.
Agalar
F
F
Department of General Surgery, Suleyman Demirel University Faculty of Medicine, Isparta, Turkey. fatihagalar@superonline.com
Hamaloglu
E
E
Daphan
C
C
Tarim
A
A
Onur
R
R
Renda
N
N
Sayek
I
I
D000818:Animals; D002245:Carbon Dioxide; D007327:Insufflation; D010535:Laparoscopy; D007813:Laparotomy; D008297:Male; D051379:Mice; D013718:Tensile Strength; D014945:Wound Healing / Q000502:physiology*
D002245:Carbon Dioxide