Localization of a blood pressure QTL on rat chromosome 1 using Dahl rat congenic strains.
We previously reported that markers on rat chromosome 1 are genetically linked to blood pressure in an F(2) population derived from Dahl salt hypertension-sensitive (S) and Lewis (LEW) rats. Because there was evidence for more than one blood pressure quantitative trait locus (QTL) on chromosome 1, an initial congenic strain introgressing a large 118-centimorgan (cM) segment of LEW chromosome 1 into the S background had been constructed. This initial congenic strain had a reduced blood pressure compared with S rats, proving the existence of a blood pressure QTL, but not giving a good localization of the QTL. In the present work a series of five overlapping congenic substrains were produced from the original congenic strain in order to localize a blood pressure QTL to a 25-cM region near the center of chromosome 1. The congenic substrains also ruled out the Sa locus as a blood pressure QTL in the S vs. LEW comparison because the Sa locus was contained in a congenic substrain that did not alter blood pressure.
Saad
Y
Y
Department of Physiology and Molecular Medicine, Medical College of Ohio, Toledo, Ohio 43614-5804, USA.
Garrett
M R
MR
Lee
S J
SJ
Dene
H
H
Rapp
J P
JP
D000818:Animals; D020296:Animals, Congenic; D001794:Blood Pressure / Q000235:genetics* / Q000502:physiology; D015152:Blotting, Northern; D001835:Body Weight; D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics*; D003066:Coenzyme A Ligases; D004247:DNA / Q000235:genetics; D005260:Female; D006321:Heart / Q000033:anatomy & histology; D007668:Kidney / Q000378:metabolism; D008297:Male; D018895:Microsatellite Repeats; D009929:Organ Size; D011506:Proteins / Q000235:genetics; D019655:Quantitative Trait, Heritable; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D020303:Rats, Inbred Dahl; D011917:Rats, Inbred Lew; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
D011506:Proteins; D012333:RNA, Messenger; D004247:DNA; C081817:Acsm3 protein, rat; D003066:Coenzyme A Ligases