Kinetin inhibits protein oxidation and glycoxidation in vitro.
We tested the ability of N(6)-furfuryladenine (kinetin) to protect against oxidative and glycoxidative protein damage generated in vitro by sugars and by an iron/ascorbate system. At 50 microM, kinetin was more efficient (82% inhibition) than adenine (49% inhibition) to inhibit the bovine serum albumin (BSA)-pentosidine formation in slow and fast glycation/glycoxidation models. Kinetin also inhibited the formation of BSA-carbonyls after oxidation significantly more than adenine did. However both compounds inhibited the advanced glycation end product (AGE) formation to the same extent (59-68% inhibition). At 200 microM, kinetin but not adenine, limited the aggregation of BSA during glycation. These data suggest that kinetin is a strong inhibitor of oxidative and glycoxidative protein-damage generated in vitro.
Verbeke
P
P
Department of Molecular and Structural Biology, Danish Centre for Molecular Gerontology, Laboratory of Cellular Ageing, Gustav Wieds Vej 10-C, DK-8000, Denmark.
Siboska
G E
GE
Clark
B F
BF
Rattan
S I
SI
D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000818:Animals; D001089:Arabinose / Q000494:pharmacology; D001120:Arginine / Q000031:analogs & derivatives* / Q000378:metabolism; D002417:Cattle; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D006037:Glyoxal / Q000494:pharmacology; D007701:Kinetin; D008239:Lysine / Q000031:analogs & derivatives* / Q000378:metabolism; D016877:Oxidants / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D011489:Protein Denaturation / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D012266:Ribose / Q000494:pharmacology; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism*; D013997:Time Factors
D017127:Glycation End Products, Advanced; D016877:Oxidants; D017382:Reactive Oxygen Species; D012709:Serum Albumin; D006037:Glyoxal; D012266:Ribose; D001120:Arginine; D001089:Arabinose; C062187:pentosidine; D000225:Adenine; D008239:Lysine; D007701:Kinetin