HLA-B44 subtyping in the Catalan population using reference strand mediated conformation analysis. Implications for the selection of unrelated bone marrow donors.
Cytotoxic T lymphocytes (CTLs) reactive against the disparity between HLA-B*4402 and HLA-B*4403 have been reported after unrelated donor bone marrow transplantation. These CTLs have been associated with acute graft-versus-host disease and graft rejection. This study describes the HLA-B44-subtyping in the Catalan population using reference-strand mediated conformation analysis. It has been performed on 297 unrelated HLA-B44+ cord blood units from the Barcelona Cord Blood Bank (Barcelona, Spain). We have found a predominance of HLA-B*4403 (66.04%) over HLA-B*4402 (33.02%), whereas the predominant HLA-B44 allele in Northern Europe and the United States is HLA-B*4402. This inverted proportion between HLA-B44 subtypes in Mediterranean populations compared with other Caucasian populations suggests that HLA-B44 subtyping should be performed when an HLA-B44+ unrelated donor marrow is identified.
Gallardo
D
D
Clinical Hematology Department, Institut Català d'Oncologia, Hospital Durán i Reynals, Barcelona, Spain.
Aróstegui
J I
JI
Rodríguez-Luaces
M
M
Querol
S
S
González
J R
JR
García-López
J
J
Grañena
A
A
D000483:Alleles; D016026:Bone Marrow Transplantation / Q000276:immunology*; D005312:Fetal Blood / Q000276:immunology; D015235:HLA-B Antigens / Q000032:analysis / Q000235:genetics* / Q000276:immunology*; D059948:HLA-B44 Antigen; D006801:Humans; D016130:Immunophenotyping / Q000379:methods; D018807:Polymorphism, Single-Stranded Conformational; D013030:Spain; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014019:Tissue Donors; D044465:White People / Q000235:genetics
D015235:HLA-B Antigens; D059948:HLA-B44 Antigen