Cleavage of yeast tRNAPhe with complementary oligonucleotide conjugated to a small ribonuclease mimic.
An oligonucleotide conjugate bearing a chemical construct mimicking the catalytic center of ribonuclease A has been designed and studied. The conjugate efficiently cleaves yeast tRNAPhe at a single site adjacent to the target complementary sequence.
Beloglazova
N G
NG
Institute of Bioorganic Chemistry, Siberian Branch of Russian Academy of Sciences, 8 Lavrentiev Avenue, 630090, Novosibirsk, Russia.
Sil'nikov
V N
VN
Zenkova
M A
MA
Vlassov
V V
VV
D000873:Anthracenes / Q000737:chemistry; D001483:Base Sequence; D002384:Catalysis / Q000187:drug effects; D006868:Hydrolysis / Q000187:drug effects; D007093:Imidazoles / Q000737:chemistry; D018716:Molecular Mimicry; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis / Q000737:chemistry*; D018075:RNA, Complementary / Q000737:chemistry*; D012331:RNA, Fungal / Q000737:chemistry; D012360:RNA, Transfer, Phe / Q000037:antagonists & inhibitors / Q000737:chemistry*; D012260:Ribonucleases / Q000737:chemistry*; D012441:Saccharomyces cerevisiae; D013873:Thionucleotides / Q000737:chemistry
C122082:9-(aminomethyl)-9,10-dihydroanthracene; D000873:Anthracenes; D007093:Imidazoles; D016376:Oligonucleotides, Antisense; D018075:RNA, Complementary; D012331:RNA, Fungal; D012360:RNA, Transfer, Phe; D013873:Thionucleotides; D012260:Ribonucleases