[Turbidimetric study of latex agglutination in 2,4-dichlorophenoxyacetic acid analysis].
By the example of 2,4-dichlorophenoxyacetic acid (2,4-D) assay by the inhibition of latex agglutination, new synthetic polymer microspheres for the conjugation with antibodies to 2,4-D and agglutinators based on ovalbumin and polyacrylamide were developed and characterized. The effect of various parameters (the concentration of reagents, the type and the degree of modification of the microsphere surface, and the nature of the carrier in the composition of the agglutinator) on the rate of agglutination and the maximal optical absorption observed during the reaction were studied by turbidimetry. The optimal parameters were found for the assay of 2,4-D by the inhibition of latex agglutination with turbidimetric registration of the results.
Genaralova
A N
AN
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Buriakov
A N
AN
Lukin
Iu V
IuV
Zubov
V P
VP
D015084:2,4-Dichlorophenoxyacetic Acid / Q000032:analysis*; D000180:Acrylic Resins; D006241:Haptens / Q000032:analysis*; D006540:Herbicides / Q000032:analysis*; D007841:Latex Fixation Tests; D008863:Microspheres; D009391:Nephelometry and Turbidimetry; D010047:Ovalbumin; D011137:Polystyrenes
D000180:Acrylic Resins; D006241:Haptens; D006540:Herbicides; D011137:Polystyrenes; D015084:2,4-Dichlorophenoxyacetic Acid; D010047:Ovalbumin