[Determination of antimicrobial, 10,10'-oxybis-10H-phenoxarsine, in artificial leather used for the seat of chairs].
Recently, a case of patients with allergic contact dermatitis caused by the poly (vinyl chloride) (PVC) seat, containing 10,10'-oxybis-10H-phenoxarsine (OBPA), of a chair was reported. OBPA was developed as an antimicrobial for plastics such as PVC and polyurethane, and it has been widely used in artificial leather for the seats of chairs and sofas. To identify causative chemicals for allergic contact dermatitis, a combination of patch testing in the patients and chemical analysis of causative products is valuable. However, no analytical method and data of OBPA in commercial products was reported. In this study, a method for the determination of OBPA in the artificial leather (PVV base) was developed. OBPA was extracted from PVC samples with methanol. The extract was loaded on an aluminum oxide column, and washed with diethyl ether:hexane, and eluted with ethanol:hexane. The eluate was evaporated, dissolved in methanol and injected to a HPLC equipped an ODS column and an UV detector (detection wavelength 300 nm). OBPA standard crystal was isolated from commercial agents containing OBPA. The calibration curve for OBPA was linear in the range of 0.1-100 micrograms/ml. The minimum detection and determination concentrations of OBPA in samples were 0.07 and 0.25 microgram/g. By this method, eight PVC sheets for the seat of a chair were analyzed. In two PVC sheets, 52.7 and 84.9 micrograms/g of OBPA were detected. In the PVC product that caused contact dermatitis, OBPA was not found.
Ikarashi
Y
Y
Division of Medical Devices, National Institute of Health Sciences, Tokyo, Japan.
Kaniwa
M
M
Nakamura
A
A
D001152:Arsenicals / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005659:Fungicides, Industrial / Q000032:analysis*; D007374:Interior Design and Furnishings; D011143:Polyvinyl Chloride
D001152:Arsenicals; D005659:Fungicides, Industrial; C017617:phenoxarsine oxide; D011143:Polyvinyl Chloride