title: The cloning of a new peroxidase found in lignocellulose cultures of Pleurotus eryngii and sequence comparison with other fungal peroxidases.
abstract: We report cloning and sequencing of gene ps1 encoding a versatile peroxidase combining catalytic properties of lignin peroxidase (LiP) and manganese peroxidase (MnP) isolated from lignocellulose cultures of the white-rot fungus Pleurotus eryngii. The gene contains 15 putative introns, and the deduced amino acid sequence consists of a 339-residue mature protein with a 31-residue signal peptide. Several putative response elements were identified in the promoter region. Amino acid residues involved in oxidation of Mn(2+) and aromatic substrates by direct electron transfer to heme and long-range electron transfer from superficial residues as predicted by analogy with Phanerochaete chrysosporium MnP and LiP, respectively. A dendrogram is presented illustrating sequence relationships between 29 fungal peroxidases.
authors.affiliation: Centro de Investigaciones Biológicas, CSIC, Velázquez 144, E-28006, Madrid, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Camarero
authors.forename: F J
authors.initials: FJ
authors.lastname: Ruiz-Dueñas
authors.forename: S
authors.initials: S
authors.lastname: Sarkar
authors.forename: M J
authors.initials: MJ
authors.lastname: Martínez
authors.forename: A T
authors.initials: AT
authors.lastname: Martínez
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002482:Cellulose / Q000378:metabolism*; D003001:Cloning, Molecular; D003470:Culture Media; D005658:Fungi / Q000201:enzymology / Q000235:genetics; D005800:Genes, Fungal; D008031:Lignin / Q000378:metabolism*; D008969:Molecular Sequence Data; D009195:Peroxidase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020076:Pleurotus / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D003470:Culture Media; C036909:lignocellulose; D002482:Cellulose; D008031:Lignin; D009195:Peroxidase