Acute cellular interaction with textured surfaces in blood contact.
Textured blood-contacting surfaces can promote the formation of a blood-compatible pseudo-neointima. We hypothesized that by controlling the surface texturing, the pseudo-neointima thickness could be controlled. The hypothesis was tested experimentally by fabricating the polyurethane textured surfaces with three different fiber lengths, and exposing them simultaneously to the flowing blood in an ovine ex vivo carotid-jugular series shunt for periods up to 4 h. The textured surface consisted of regularly spaced tapered micro-fibers of defined length on a smooth base-plane surface. Because of the simple surface topography, detailed computational fluid-dynamic modeling of the surface could be obtained as a parallel study. Experimental results showed that white cell was the predominant cell type deposited on the textured surfaces, whereas macroscopic thrombus formation occurred only in one of nine blood-contacting experiments. White cell density on the textured base-plane surface was subsequently quantified by image-analyzing the electron micrographs of blood-contacted textured surfaces. The statistical analysis of cell densities on individual textured surfaces showed effects of wall shear stress on the textured base plane (which was obtained from the fluid-dynamic modeling), the longitudinal position of the test section in the series shunt, and blood-contact time.
Fujisawa
N
N
Graduate School of Biomedical Engineering, University of New South Wales, Sydney 2052, Australia. n.fujisawa@unsw.edu.au
Odell
R A
RA
Poole-Warren
L A
LA
Bertram
C D
CD
Woodard
J C
JC
Schindhelm
K
K
D000818:Animals; D001166:Arteriovenous Shunt, Surgical / Q000295:instrumentation / Q000379:methods; D001672:Biocompatible Materials; D001773:Blood Cells / Q000648:ultrastructure*; D001792:Blood Platelets / Q000187:drug effects / Q000648:ultrastructure; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D003198:Computer Simulation; D004912:Erythrocytes / Q000187:drug effects / Q000648:ultrastructure; D007962:Leukocytes / Q000187:drug effects / Q000648:ultrastructure; D016014:Linear Models; D008855:Microscopy, Electron, Scanning; D008955:Models, Cardiovascular; D011140:Polyurethanes / Q000737:chemistry; D012756:Sheep; D013314:Stress, Mechanical; D013499:Surface Properties; D013927:Thrombosis / Q000473:pathology
D001672:Biocompatible Materials; D011140:Polyurethanes