title: Antisense silencing of the creA gene in Aspergillus nidulans.
abstract: Antisense expression of a portion of the gene encoding the major carbon catabolite repressor CREA in Aspergillus nidulans resulted in a substantial increase in the levels of glucose-repressible enzymes, both endogenous and heterologous, in the presence of glucose. The derepression effect was approximately one-half of that achieved in a null creA mutant. Unlike results for that mutant, however, growth parameters and colony morphology in the antisense transformants were not affected.
authors.affiliation: Center for Process Biotechnology, Department of Biotechnology, Technical University of Denmark, 2800 Lyngby, Denmark.
authors.forename: L F
authors.initials: LF
authors.lastname: Bautista
authors.forename: A
authors.initials: A
authors.lastname: Aleksenko
authors.forename: M
authors.initials: M
authors.lastname: Hentzer
authors.forename: A
authors.initials: A
authors.lastname: Santerre-Henriksen
authors.forename: J
authors.initials: J
authors.lastname: Nielsen
mesh_terms: D001233:Aspergillus nidulans / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D005285:Fermentation; D005656:Fungal Proteins; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D020868:Gene Silencing; D005947:Glucose / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism; D016372:RNA, Antisense / Q000494:pharmacology*; D012097:Repressor Proteins / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects; D000516:alpha-Amylases / Q000235:genetics / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D016372:RNA, Antisense; D012097:Repressor Proteins; C071237:CreA protein, Aspergillus nidulans; C074451:Endo-arabinase; D006026:Glycoside Hydrolases; D000516:alpha-Amylases; D005947:Glucose