title: Delivery systems for antisense oligonucleotides.
abstract: In vitro, the efficacy of the antisense approach is strongly increased by systems delivering oligodeoxyribonucleotides (ODNs) to cells. Up to now, most of the developed vectors favor ODN entrance by a mechanism based on endocytosis. Such is the case for particulate systems, including liposomes (cationic or non-cationic), cationic polyelectrolytes, and delivery systems targeted to specific receptors. Under these conditions, endosomal compartments may represent a dead end for ODNs. Current research attempts to develop conditions for escaping from these compartments. A new class of vectors acts by passive permeabilization of the plasma membrane. It includes peptides, streptolysin O, and cationic derivatives of polyene antibiotics. In vivo, the interest of a delivery system, up to now, has appeared limited. Development of vectors insensitive to the presence of serum seems to be a prerequisite for future improvements.
authors.affiliation: LPBC, CNRS ESA 7033, Université Pierre et Marie Curie, case 138, 4, place Jussieu, 75252 05, Paris cedex, France.
authors.forename: C
authors.initials: C
authors.lastname: Garcia-Chaumont
authors.forename: O
authors.initials: O
authors.lastname: Seksek
authors.forename: J
authors.initials: J
authors.lastname: Grzybowska
authors.forename: E
authors.initials: E
authors.lastname: Borowski
authors.forename: J
authors.initials: J
authors.lastname: Bolard
mesh_terms: D000666:Amphotericin B / Q000378:metabolism; D002462:Cell Membrane / Q000502:physiology; D016503:Drug Delivery Systems; D004705:Endocytosis; D015316:Genetic Therapy; D006801:Humans; D008081:Liposomes; D008823:Micelles; D016376:Oligonucleotides, Antisense / Q000008:administration & dosage*; D010539:Permeability
chemical_list: D008081:Liposomes; D008823:Micelles; D016376:Oligonucleotides, Antisense; D000666:Amphotericin B