title: Induction of minisatellite mutations in the mouse germline by low-dose chronic exposure to gamma-radiation and fission neutrons.
abstract: Germline mutation induction at mouse minisatellite loci by paternal low-dose (0.125-1 Gy) exposure to chronic (1.66 x 10(-4) Gy min(-1)) low-linear energy transfer (low-LET) gamma-irradiation and high-LET fission neutrons (0.003 Gy min(-1)) was studied at pre-meiotic stages of spermatogenesis. Both types of radiation produced linear dose-response curves for mutation of the paternal allele. In contrast to previous results using higher doses, the pattern of induction of minisatellite mutation after chronic gamma-irradiation was similar to acute (0.5 Gy min(-1)) exposure to X-rays, indicating that the elevated mutation rate was independent of the ability of the cell to repair damage induced immediately or over a period of up to 100 h. Chronic exposure to fission neutrons was more effective than acute or chronic low-LET exposure (relative biological effectiveness, RBE=3.36). The data also provide strong support for the previous conclusion that increases in minisatellite mutation rate are not caused by radiation-induced DNA damage at minisatellite loci themselves, but rather from damage induced by ionising radiation elsewhere in the genome/cell.
authors.affiliation: Department of Genetics, University of Leicester, Leicester LE1 7RH, UK. yed2@le.ac.uk
authors.forename: Y E
authors.initials: YE
authors.lastname: Dubrova
authors.forename: M
authors.initials: M
authors.lastname: Plumb
authors.forename: J
authors.initials: J
authors.lastname: Brown
authors.forename: E
authors.initials: E
authors.lastname: Boulton
authors.forename: D
authors.initials: D
authors.lastname: Goodhead
authors.forename: A J
authors.initials: AJ
authors.lastname: Jeffreys
mesh_terms: D000818:Animals; D005720:Gamma Rays; D018095:Germ-Line Mutation; D018499:Linear Energy Transfer; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D018598:Minisatellite Repeats / Q000235:genetics / Q000528:radiation effects*; D009502:Neutrons; D018812:Paternal Exposure