A screen for new trithorax group genes identified little imaginal discs, the Drosophila melanogaster homologue of human retinoblastoma binding protein 2.
The proteins encoded by two groups of conserved genes, the Polycomb and trithorax groups, have been proposed to maintain, at the level of chromatin structure, the expression pattern of homeotic genes during Drosophila development. To identify new members of the trithorax group, we screened a collection of deficiencies for intergenic noncomplementation with a mutation in ash1, a trithorax group gene. Five of the noncomplementing deletions uncover genes previously classified as members of the Polycomb group. This evidence suggests that there are actually three groups of genes that maintain the expression pattern of homeotic genes during Drosophila development. The products of the third group appear to be required to maintain chromatin in both transcriptionally inactive and active states. Six of the noncomplementing deficiencies uncover previously unidentified trithorax group genes. One of these deficiencies removes 25D2-3 to 26B2-5. Within this region, there are two, allelic, lethal P-insertion mutations that identify one of these new trithorax group genes. The gene has been called little imaginal discs based on the phenotype of mutant larvae. The protein encoded by the little imaginal discs gene is the Drosophila homologue of human retinoblastoma binding protein 2.
Gildea
J J
JJ
Department of Biology, The Johns Hopkins University, Baltimore, Maryland 21218, USA.
Lopez
R
R
Shearn
A
A
D000818:Animals; D002352:Carrier Proteins / Q000235:genetics*; D002843:Chromatin / Q000235:genetics; D003433:Crosses, Genetic; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000033:anatomy & histology* / Q000235:genetics* / Q000254:growth & development; D005260:Female; D005801:Genes, Homeobox; D005804:Genes, Lethal; D005838:Genotype; D006801:Humans; D019476:Insect Proteins / Q000235:genetics*; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D063150:Polycomb Repressive Complex 1; D012097:Repressor Proteins / Q000235:genetics; D016160:Retinoblastoma Protein / Q000378:metabolism; D056485:Retinoblastoma-Binding Protein 2; D013489:Suppression, Genetic; D013909:Thorax; D014158:Transcription, Genetic; D025521:Tumor Suppressor Proteins
D002352:Carrier Proteins; D002843:Chromatin; D029721:Drosophila Proteins; D019476:Insect Proteins; D047908:Intracellular Signaling Peptides and Proteins; C066770:Pc protein, Drosophila; D012097:Repressor Proteins; D016160:Retinoblastoma Protein; D025521:Tumor Suppressor Proteins; C070050:KDM5A protein, human; D056485:Retinoblastoma-Binding Protein 2; D063150:Polycomb Repressive Complex 1