Association effects of vitamin B6 and various magnesium salts on a pharmacological model: the human amniotic membrane.
The effects of the association of vitamin B6 and Mg salts: aspartate, citrate, lactate, pidolate, sulfate, were studied on the ionic transfer through a membraneous pharmacological model: the isolated human amniotic membrane. The ionic transfer was evaluated by measure of the total conductance in the maternal to fetal way (GtM) and in the fetal to maternal way (GtF), of the ionic fluxes (F1 on the maternal side, F2 on the fetal side) and of the ratio F1/F2. The results were explained in terms of monophasic (decrease: screening or increase: binding interactions with the polar surface moities) or biphasic (decrease then increase) action. The results indicated: Mg aspartate decreased GtF, F1, F2 whatever concentration and had a concentration-dependent effect on GtM, F1/F2. The addition of vitamin B6 induced a new concentration-dependent effect (biphasic action: decrease then increase) on GtF, F1 and also modified F2, F1/F2. Mg citrate decreased GtM, GtF, F2 whatever the concentration and had a concentration-dependent effect on F1, F1/F2. The addition of vitamin B6 induced a new biphasic effect on GtM and GtF. Mg lactate decreased GtM, F1, F2, F1/F2 whatever the concentration but had a concentration-dependent effect on GtF. The addition of vitamin B6 induced a new biphasic effect on GtM, F1, F2, F1/F2. Mg pidolate had no effect on GtM, GtF, F2, F1/F2 and decreased F1. The addition of vitamin B6 did not induce variation. Mg sulfate had no effect on GtM, increased GtF and decreased F1, F2, F1/F2. The addition of vitamin B6 induced a new concentration-dependent effect on F2. The association between vitamin B6 and Mg salts implicated a new action on components of the amniotic ionic transfer characterized by a biphasic action (decrease then increase concentration-dependent effect). This effect was dependent on the anion associated with magnesium. The magnesium salts may be classified with regard to the beneficial effect due to the association with vitamin B6 in the following decreasing order: aspartate and lactate, citrate, pidolate, sulfate.
Bara
M
M
Laboratory of Physiopathology de l'Implantation et du Développement, University P.M. Curie, Paris, France. Michel.Bara@snv.jussieu.fr
Guiet-Bara
A
A
Durlach
J
J
D000650:Amnion / Q000187:drug effects* / Q000378:metabolism; D000704:Analysis of Variance; D004594:Electrophysiology; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D017136:Ion Transport / Q000187:drug effects*; D017616:Magnesium Compounds / Q000494:pharmacology*; D011247:Pregnancy; D011736:Pyridoxine / Q000494:pharmacology*
D017616:Magnesium Compounds; D011736:Pyridoxine