title: Induction of primary NY-ESO-1 immunity: CD8+ T lymphocyte and antibody responses in peptide-vaccinated patients with NY-ESO-1+ cancers.
abstract: Cancer-testis antigen NY-ESO-1 is one of the most immunogenic tumor antigens defined to date. Spontaneous humoral and CD8+ T-cell responses to NY-ESO-1 are detected in 40-50% of patients with advanced NY-ESO-1-expressing tumors. A clinical trial was initiated to study the immunological effects of intradermal vaccination with 3 HLA-A2-binding NY-ESO-1 peptides in 12 patients with metastatic NY-ESO-1-expressing cancers. Seven patients were NY-ESO-1 serum antibody negative, and five patients were NY-ESO-1 serum antibody positive at the outset of the study. Primary peptide-specific CD8+ T-cell reactions and delayed-type hypersensitivity responses were generated in four of seven NY-ESO-1 antibody-negative patients. Induction of a specific CD8+ T-cell response to NY-ESO-1 in immunized antibody-negative patients was associated with disease stabilization and objective regression of single metastases. NY-ESO-1 antibody-positive patients did not develop significant changes in baseline NY-ESO-1-specific T-cell reactivity. However, stabilization of disease and regression of individual metastases were observed in three of five immunized patients. These results demonstrate that primary NY-ESO-1-specific CD8+ T-cell responses can be induced by intradermal immunization with NY-ESO-1 peptides, and that immunization with NY-ESO-1 may have the potential to alter the natural course of NY-ESO-1-expressing tumors.
authors.affiliation: II. Medizinische Klinik, Hämatologie-Onkologie, Krankenhaus Nordwest, 60488 Frankfurt, Germany. EJ200161@AOL.com
authors.forename: E
authors.initials: E
authors.lastname: Jäger
authors.forename: S
authors.initials: S
authors.lastname: Gnjatic
authors.forename: Y
authors.initials: Y
authors.lastname: Nagata
authors.forename: E
authors.initials: E
authors.lastname: Stockert
authors.forename: D
authors.initials: D
authors.lastname: Jäger
authors.forename: J
authors.initials: J
authors.lastname: Karbach
authors.forename: A
authors.initials: A
authors.lastname: Neumann
authors.forename: J
authors.initials: J
authors.lastname: Rieckenberg
authors.forename: Y T
authors.initials: YT
authors.lastname: Chen
authors.forename: G
authors.initials: G
authors.lastname: Ritter
authors.forename: E
authors.initials: E
authors.lastname: Hoffman
authors.forename: M
authors.initials: M
authors.lastname: Arand
authors.forename: L J
authors.initials: LJ
authors.lastname: Old
authors.forename: A
authors.initials: A
authors.lastname: Knuth
mesh_terms: D000595:Amino Acid Sequence; D000912:Antibodies, Neoplasm / Q000096:biosynthesis*; D000951:Antigens, Neoplasm / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D019496:Cancer Vaccines / Q000008:administration & dosage* / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D006801:Humans; D006968:Hypersensitivity, Delayed; D008297:Male; D008565:Membrane Proteins; D010455:Peptides / Q000008:administration & dosage / Q000276:immunology; D011506:Proteins / Q000276:immunology*; D013737:Testis / Q000276:immunology*
chemical_list: D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; C104948:CTAG1B protein, human; D019496:Cancer Vaccines; D008565:Membrane Proteins; D010455:Peptides; D011506:Proteins