Survivability and immune responses after challenge with Edwardsiella ictaluri in susceptible and resistant families of channel catfish, Ictalurus punctatus.
Diseases in catfish farming are prevalent and costly, particularly the bacterial disease Enteric Septicemia of Catfish. Considerable research has focused on different aspects of this disease, including the biology of the causative agent, Edwardsiella ictaluri. However, no satisfactory treatment or preventive has resulted from these efforts. One solution is to increase the natural disease resistance of the fish through genetic selection. Recent research has demonstrated that genetic factors influence resistance to infection in mammals as well as fish. Selective breeding for disease resistance in channel catfish is ongoing, however differences in defence mechanisms among E. ictaluri challenged strains and families are only now being investigated. Antigen-specific as well as non-specific immune responses of full-sib families of channel catfish to laboratory challenge with E. ictaluri have been investigated. Both resistant and sensitive families produce a humoral response as specific antibody, but there were no differences found in the level of specific antibody produced. The sensitive family produced a slightly higher percentage of B lymphocytes in mononuclear cell preparations from peripheral blood, while the resistant family had a higher percentage of T lymphocytes in those preparations. The most significant observation was that the resistant family produced more macrophage aggregations in the spleen and posterior kidney throughout the infection than the sensitive family. Neither family produced stress-associated amounts of cortisol.
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Thad Cochran National Warmwater Aquaculture Center, College of Veterinary Medicine, Mississippi State University, Stoneville, USA.
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D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000911:Antibodies, Monoclonal; D017756:Aquaculture; D004198:Disease Susceptibility / Q000382:microbiology / Q000662:veterinary; D020594:Edwardsiella / Q000276:immunology* / Q000472:pathogenicity; D004756:Enterobacteriaceae Infections / Q000235:genetics / Q000276:immunology / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005393:Fish Diseases / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D005434:Flow Cytometry / Q000662:veterinary; D020022:Genetic Predisposition to Disease; D006651:Histocytochemistry; D006854:Hydrocortisone / Q000097:blood; D007059:Ictaluridae; D007113:Immunity, Innate / Q000235:genetics / Q000276:immunology; D007136:Immunoglobulins / Q000097:blood; D007668:Kidney / Q000473:pathology; D016018:Least-Squares Analysis; D008099:Liver / Q000473:pathology; D016131:Lymphocyte Subsets; D008264:Macrophages; D011863:Radioimmunoassay / Q000662:veterinary; D011897:Random Allocation; D015144:Southeastern United States; D013154:Spleen / Q000473:pathology
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D007136:Immunoglobulins; D006854:Hydrocortisone