Interaction of the rabies virus P protein with the LC8 dynein light chain.
The rabies virus P protein is involved in viral transcription and replication but its precise function is not clear. We investigated the role of P (CVS strain) by searching for cellular partners by using a two-hybrid screening of a PC12 cDNA library. We isolated a cDNA encoding a 10-kDa dynein light chain (LC8). LC8 is a component of cytoplasmic dynein involved in the minus end-directed movement of organelles along microtubules. We confirmed that this molecule interacts with P by coimmunoprecipitation in infected cells and in cells transfected with a plasmid encoding P protein. LC8 was also detected in virus particles. Series of deletions from the N- and C-terminal ends of P protein were used to map the LC8-binding domain to the central part of P (residues 138 to 172). These results are relevant to speculate that dynein may be involved in the axonal transport of rabies virus along microtubules through neuron cells.
Raux
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H
Laboratoire de Génétique des Virus, CNRS, 91198 Gif sur Yvette, France.
Flamand
A
A
Blondel
D
D
D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D018076:DNA, Complementary; D029721:Drosophila Proteins; D004398:Dyneins; D015723:Gene Library; D018832:Molecular Chaperones; D009414:Nerve Growth Factors / Q000378:metabolism; D016716:PC12 Cells; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D011233:Precipitin Tests; D011820:Rabies virus / Q000235:genetics* / Q000502:physiology*; D051381:Rats; D014162:Transfection; D020798:Two-Hybrid System Techniques; D015678:Viral Structural Proteins / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication
D002352:Carrier Proteins; D018076:DNA, Complementary; D029721:Drosophila Proteins; D018832:Molecular Chaperones; D009414:Nerve Growth Factors; C103961:P phosphoprotein, Rabies virus; D010750:Phosphoproteins; D015678:Viral Structural Proteins; D004398:Dyneins