A calmodulin-related protein that suppresses posttranscriptional gene silencing in plants.
Posttranscriptional gene silencing (PTGS) is an ancient eukaryotic regulatory mechanism in which a particular RNA sequence is targeted and destroyed. The helper component-proteinase (HC-Pro) of plant potyviruses suppresses PTGS in plants. Using a yeast two-hybrid system, we identified a calmodulin-related protein (termed rgs-CaM) that interacts with HC-Pro. Here we report that rgs-CaM, like HC-Pro itself, suppresses gene silencing. Our work is the first report identifying a cellular suppressor of PTGS.
Anandalakshmi
R
R
Department of Biological Sciences, University of South Carolina, Columbia, SC 29208, USA.
Marathe
R
R
Ge
X
X
Herr
J M
JM
Mau
C
C
Mallory
A
A
Pruss
G
G
Bowman
L
L
Vance
V B
VB
D016960:Agrobacterium tumefaciens / Q000235:genetics; D000595:Amino Acid Sequence; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D020868:Gene Silencing; D017343:Genes, Plant; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010941:Plant Tumors / Q000235:genetics; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D010957:Plasmids; D017863:Potexvirus / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D019076:Transgenes; D014764:Viral Proteins / Q000378:metabolism*
D008164:Luminescent Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D014764:Viral Proteins; C414615:rgs-CaM protein, Nicotiana tabacum; D049452:Green Fluorescent Proteins; D003546:Cysteine Endopeptidases; C072775:HC-Pro protein, potyvirus