title: Compromised energetics in the adenylate kinase AK1 gene knockout heart under metabolic stress.
abstract: Rapid exchange of high energy carrying molecules between intracellular compartments is essential in sustaining cellular energetic homeostasis. Adenylate kinase (AK)-catalyzed transfer of adenine nucleotide beta- and gamma-phosphoryls has been implicated in intracellular energy communication and nucleotide metabolism. To demonstrate the significance of this reaction in cardiac energetics, phosphotransfer dynamics were determined by [(18)O]phosphoryl oxygen analysis using( 31)P NMR and mass spectrometry. In hearts with a null mutation of the AK1 gene, which encodes the major AK isoform, total AK activity and beta-phosphoryl transfer was reduced by 94% and 36%, respectively. This was associated with up-regulation of phosphoryl flux through remaining minor AK isoforms and the glycolytic phosphotransfer enzyme, 3-phosphoglycerate kinase. In the absence of metabolic stress, deletion of AK1 did not translate into gross abnormalities in nucleotide levels, gamma-ATP turnover rate or creatine kinase-catalyzed phosphotransfer. However, under hypoxia AK1-deficient hearts, compared with the wild type, had a blunted AK-catalyzed phosphotransfer response, lowered intracellular ATP levels, increased P(i)/ATP ratio, and suppressed generation of adenosine. Thus, although lack of AK1 phosphotransfer can be compensated in the absence of metabolic challenge, under hypoxia AK1-knockout hearts display compromised energetics and impaired cardioprotective signaling. This study, therefore, provides first direct evidence that AK1 is essential in maintaining myocardial energetic homeostasis, in particular under metabolic stress.
authors.affiliation: Division of Cardiovascular Diseases, Departments of Medicine, Molecular Pharmacology and Experimental Therapeutics, and Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota 55905, USA.
authors.forename: D
authors.initials: D
authors.lastname: Pucar
authors.forename: E
authors.initials: E
authors.lastname: Janssen
authors.forename: P P
authors.initials: PP
authors.lastname: Dzeja
authors.forename: N
authors.initials: N
authors.lastname: Juranic
authors.forename: S
authors.initials: S
authors.lastname: Macura
authors.forename: B
authors.initials: B
authors.lastname: Wieringa
authors.forename: A
authors.initials: A
authors.lastname: Terzic
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis; D000263:Adenylate Kinase / Q000235:genetics / Q000502:physiology*; D000818:Animals; D004734:Energy Metabolism; D006706:Homeostasis; D051379:Mice; D018345:Mice, Knockout; D009206:Myocardium / Q000378:metabolism*
chemical_list: D000255:Adenosine Triphosphate; D000263:Adenylate Kinase