title: Reliability of lymphoscintigraphy in indicating the number of sentinel nodes in melanoma patients.
abstract: BACKGROUND
  This study was undertaken to establish the reliability of lymphoscintigraphy in indicating the number of sentinel nodes in patients with melanoma.
  
  
  METHODS
  Lymphoscintigraphy was performed with dynamic imaging after injection of 60 MBq 99mTc-nanocolloid (1.6 mCi) and static imaging after 2 hours in 200 patients with clinically localized primary melanoma of the skin. The following day, sentinel nodes were retrieved with the blue dye technique and a gamma detection probe (Neoprobe 1000/1500). The discrepancies between the number of sentinel nodes indicated by lymphoscintigraphy and the actual number of sentinel nodes as established by the surgeon were evaluated.
  
  
  RESULTS
  Lymphoscintigraphy showed drainage to 393 sentinel nodes in 255 lymphatic fields in 199 patients. In 48 lymphatic fields (19%) in 46 patients (23%), the number of sentinel nodes was different from the number that was visualized with scintigraphy. Additional sentinel nodes were found by the surgeon because a lymphatic vessel was not seen on the lymphoscintigraphy (43%), because a sentinel node was not visualized separately from other hot nodes or vessels or the injection site (36%), or because a sentinel node was blue and not hot (4%). Fewer sentinel nodes were found than suggested by scintigraphy because a lymphangioma was mistaken for a sentinel node (4%) or because a single elongated node was depicted as two hot spots (6%).
  
  
  CONCLUSIONS
  Although lymphoscintigraphy is indispensable for lymphatic mapping, the predicted number of sentinel nodes is accurate in only 81% of lymph node fields. The limited discriminating power of the gamma camera is an important cause of discrepancies.
authors.affiliation: Department of Surgery, The Netherlands Cancer Institute/Antoni van Leeuwenhoek Hospital, Amsterdam. liesbeth.jansen@wxs.nl
authors.forename: L
authors.initials: L
authors.lastname: Jansen
authors.forename: O E
authors.initials: OE
authors.lastname: Nieweg
authors.forename: A E
authors.initials: AE
authors.lastname: Kapteijn
authors.forename: R A
authors.initials: RA
authors.lastname: Valdés Olmos
authors.forename: S H
authors.initials: SH
authors.lastname: Muller
authors.forename: C A
authors.initials: CA
authors.lastname: Hoefnagel
authors.forename: B B
authors.initials: BB
authors.lastname: Kroon
mesh_terms: D000293:Adolescent; D000328:Adult; D006801:Humans; D008198:Lymph Nodes / Q000000981:diagnostic imaging* / Q000473:pathology; D008207:Lymphatic Metastasis / Q000000981:diagnostic imaging; D008545:Melanoma / Q000000981:diagnostic imaging* / Q000473:pathology*; D008875:Middle Aged; D011446:Prospective Studies; D011877:Radionuclide Imaging; D015203:Reproducibility of Results; D021701:Sentinel Lymph Node Biopsy; D012878:Skin Neoplasms / Q000000981:diagnostic imaging* / Q000473:pathology*