Analysis of the promoter region of the human FcRn gene.
The 5'-flanking region of the human FcRn alpha-chain gene was analyzed for its ability to directly express the chloramphenicol acetyltransferase (CAT) reporter gene in NIH3T3 and Lu106 cells. Transient transfection of the CAT constructs revealed that there was promoter activity in the region -660 to +300 of the 5'-flanking sequence. Electrophoretic mobility-shift assays showed that there are functional binding sites for Sp1 or Sp1-like factors, AP1 or a related factor, and additional unidentified proteins in the promoter region.
Mikulska
J E
JE
Ludwik Hirszfeld Institute of Immunolgy and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland. milkulska@immuno.iitd.pan.wroc.pl
Simister
N E
NE
D005786:Gene Expression Regulation; D017930:Genes, Reporter; D015395:Histocompatibility Antigens Class I; D006801:Humans; D011401:Promoter Regions, Genetic; D011961:Receptors, Fc / Q000235:genetics*; D014157:Transcription Factors / Q000502:physiology
D015395:Histocompatibility Antigens Class I; D011961:Receptors, Fc; D014157:Transcription Factors; C100688:Fc receptor, neonatal