title: Functional regions in the essential light chain of smooth muscle myosin as revealed by the mutagenesis approach.
abstract: The endogenous essential light chain (LC17) of myosin from intestine smooth muscle was replaced with mutated essential light chains prepared using recombinant techniques. Complete exchange was observed with histidine-tagged derivatives of LC17a, LC17b and E122A-LC17a (LC17a and LC17b are the usual constituants of smooth muscle myosin), with small changes in the ATPase activity of reconstituted myosins. Much less exchange was observed with the light-chain derivative lacking the last 12 amino acid residues, demonstrating the importance of this segment, which may act as one arm of a pair of pincers to bind the myosin heavy chain.
authors.affiliation: Laboratoire des Gènes et Protéines Musculaires, Université de Paris-Sud, France. sophie.cheruel@egm.u-psud.fr
authors.forename: S
authors.initials: S
authors.lastname: Quevillon-Chéruel
authors.forename: C
authors.initials: C
authors.lastname: Janmot
authors.forename: M
authors.initials: M
authors.lastname: Nozais
authors.forename: A M
authors.initials: AM
authors.lastname: Lompré
authors.forename: J J
authors.initials: JJ
authors.lastname: Béchet
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D003106:Colon / Q000378:metabolism; D006639:Histidine; D007583:Jejunum / Q000378:metabolism; D008969:Molecular Sequence Data; D009130:Muscle, Smooth / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D018994:Myosin Light Chains / Q000737:chemistry* / Q000378:metabolism*; D009218:Myosins / Q000737:chemistry / Q000378:metabolism; D020033:Protein Isoforms / Q000737:chemistry / Q000378:metabolism; D011817:Rabbits; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D018994:Myosin Light Chains; D020033:Protein Isoforms; D011994:Recombinant Proteins; D006639:Histidine; D017301:Ca(2+) Mg(2+)-ATPase; D009218:Myosins