Regulation of T cell activation, anxiety, and male aggression by RGS2.
Regulators of G protein signaling (RGS) proteins accelerate the GTPase activity of Galpha protein subunits in vitro, negatively regulating G protein-coupled receptor signaling. The physiological role of mammalian RGS proteins is largely unknown. The RGS family member rgs2 was cloned as an immediate early response gene up-regulated in T lymphocytes after activation. To investigate the role of RGS2 in vivo, we generated rgs2-deficient mice. We show that targeted mutation of rgs2 in mice leads to reduced T cell proliferation and IL-2 production, which translates in an impaired antiviral immunity in vivo. Interestingly, rgs2(-/-) mice also display increased anxiety responses and decreased male aggression in the absence of cognitive or motor deficits. RGS2 also controls synaptic development and basal electrical activity in hippocampal CA1 neurons. Thus, RGS2 plays an important role in T cell activation, synapse development in the hippocampus, and emotive behaviors.
Oliveira-Dos-Santos
A J
AJ
Amgen Institute, University of Toronto, 620 University Avenue, Toronto, ON M5G 2C1 Canada.
Matsumoto
G
G
Snow
B E
BE
Bai
D
D
Houston
F P
FP
Whishaw
I Q
IQ
Mariathasan
S
S
Sasaki
T
T
Wakeham
A
A
Ohashi
P S
PS
Roder
J C
JC
Barnes
C A
CA
Siderovski
D P
DP
Penninger
J M
JM
D000374:Aggression / Q000502:physiology*; D000818:Animals; D001007:Anxiety / Q000503:physiopathology*; D001483:Base Sequence; D002455:Cell Division / Q000502:physiology; D017931:DNA Primers; D018390:Gene Targeting; D006624:Hippocampus / Q000166:cytology / Q000502:physiology; D008213:Lymphocyte Activation / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009474:Neurons / Q000502:physiology; D020710:RGS Proteins / Q000502:physiology*; D013569:Synapses / Q000502:physiology; D013601:T-Lymphocytes / Q000276:immunology*
D017931:DNA Primers; D020710:RGS Proteins; C105345:Rgs2 protein, mouse