title: Differential effects of neurotensin and cholecystokinin on intestinal microcirculation after ischemia-reperfusion.
abstract: BACKGROUND
  We investigated the effect of neurotensin and cholecystokinin (CCK) on intestinal microcirculation after ischemia-reperfusion.
  
  
  METHODS
  Ischemia was induced in Wistar rats by occlusion of the superior mesenteric artery for 40 min. Ten minutes before reperfusion, infusion of either neurotensin or CCK was started. Afterwards, the microhemodynamics of the jejunum were examined by means of intravital microscopy.
  
  
  RESULTS
  Ischemia-reperfusion decreased functional capillary density from 873.4+/-18.1 to 362.5+/-8.3 cm(-1) and red blood cell velocity from 0.49+/-0.03 to 0.34+/-0.02 mm/s. Furthermore, leukocyte-endothelium interaction was increased. Neurotensin infusion significantly increased functional capillary density to 483.2+/-9.0 cm(-1) and red blood cell velocity to 0.69+/-0.01 mm/s in the mucosal capillaries compared with ischemic controls. Despite the amelioration of villus perfusion, the number of non-perfused villi significantly increased (11.8+/-3.6%) compared with ischemic controls. CCK infusion also resulted in a significant increase of functional capillary density (535.2+/-7.4 cm(-1)) and red blood cell velocity (0.67+/-0.01 mm/s). In contrast to neurotensin, the number of non-perfused villi was not increased (5.8+/-2.2%).
  
  
  CONCLUSIONS
  We conclude that neurotensin further aggravates perfusion inhomogeneity and stasis when administered during the ischemic period. In contrast, CCK has no negative influence on perfusion homogeneity after ischemia-reperfusion. It may be superior to neurotensin in the reconstitution of normal microvascular perfusion patterns after ischemia-reperfusion.
authors.affiliation: Department of General Surgery, George-August-University, Göttingen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Heuser
authors.forename: O
authors.initials: O
authors.lastname: Gralla
authors.forename: O
authors.initials: O
authors.lastname: Pfaar
authors.forename: R
authors.initials: R
authors.lastname: Nustede
authors.forename: H J
authors.initials: HJ
authors.lastname: Gröne
authors.forename: S
authors.initials: S
authors.lastname: Post
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002196:Capillaries / Q000503:physiopathology; D002766:Cholecystokinin / Q000008:administration & dosage / Q000097:blood*; D005260:Female; D007413:Intestinal Mucosa / Q000098:blood supply; D007583:Jejunum / Q000098:blood supply*; D008833:Microcirculation; D009496:Neurotensin / Q000008:administration & dosage / Q000009:adverse effects / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism* / Q000503:physiopathology
chemical_list: D009496:Neurotensin; D002766:Cholecystokinin