title: Contributions of protein disulfide isomerase domains to its chaperone activity.
abstract: Protein disulfide isomerase (PDI), a member of the thioredoxin (Trx) superfamily, consists of five consecutive domains, a-b-b'-a'-c. Domain combinations, AB, A'C, B'A'C and AB-C, and hybrids of PDI domains with Trx, Trx-C and Trx-B'A'C, have been constructed and expressed in Escherichia coli to examine the contributions of PDI domains to its enzyme and chaperone activities. All the combination and hybrid products are considerably less active than intact PDI in their enzyme activities. Recombinant products containing C, at low concentrations, inhibit the reactivation of lysozyme in HEPES buffer, while those without C do not. Only the intact PDI molecule and the hybrid molecule, Trx-B'A'C, but to a much lower level, show general chaperone activity in assisting the reactivation of denatured D-glyceraldehyde-3-phosphate dehydrogenase. It is suggested that all domains of PDI contribute to the binding of target protein for its chaperone activity.
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Academia Sinica, Beijing, PR China.
authors.forename: X X
authors.initials: XX
authors.lastname: Sun
authors.forename: Y
authors.initials: Y
authors.lastname: Dai
authors.forename: H P
authors.initials: HP
authors.lastname: Liu
authors.forename: S M
authors.initials: SM
authors.lastname: Chen
authors.forename: C C
authors.initials: CC
authors.lastname: Wang
mesh_terms: D002021:Buffers; D004789:Enzyme Activation; D004926:Escherichia coli / Q000378:metabolism; D015870:Gene Expression; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D006531:HEPES; D018832:Molecular Chaperones / Q000378:metabolism*; D009113:Muramidase / Q000037:antagonists & inhibitors; D010957:Plasmids; D019704:Protein Disulfide-Isomerases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020033:Protein Isoforms / Q000737:chemistry; D013879:Thioredoxins / Q000737:chemistry
chemical_list: D002021:Buffers; D018832:Molecular Chaperones; D020033:Protein Isoforms; D013879:Thioredoxins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D009113:Muramidase; D019704:Protein Disulfide-Isomerases; D006531:HEPES