Schizosaccharomyces pombe gmd3(+)/alg11(+) is a functional homologue of Saccharomyces cerevisiae ALG11 which is involved in N-linked oligosaccharide synthesis.
The oligosaccharide of glycoproteins in the fission yeast Schizosaccharomyces pombe is unique in containing galactose. We isolated four mutants that had reduced amounts of galactose residues on their cell surface glycoproteins by fluorescence-activated cell sorter. The isolated four recessive mutants, gmd1 to gmd4, showed a defect in glycosylation of acid phosphatase, a cell surface glycoprotein. In gmd3 mutant cells, the amounts of both mannose and galactose residues were decreased on the cell surface galactomannoproteins, suggesting an underglycosylation of galactomannoproteins. The gmd3(+) gene encodes a protein that has significant similarity with Saccharomyces cerevisiae Alg11p and is likely to be involved in N-linked core oligosaccharide synthesis. ALG11 suppressed the gmd3 mutation, indicating that gmd3(+) gene is a functional homologue of the ALG11 gene. We therefore designated gmd3(+) as alg11(+).
Umeda
K
K
United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan.
Yoko-o
T
T
Nakayama
K
K
Suzuki
T
T
Jigami
Y
Y
D000135:Acid Phosphatase / Q000378:metabolism; D000595:Amino Acid Sequence; D002236:Carbohydrate Conformation; D002462:Cell Membrane / Q000737:chemistry; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D005690:Galactose / Q000032:analysis / Q000031:analogs & derivatives; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D006031:Glycosylation; D008351:Mannans / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000737:chemistry; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012568:Schizosaccharomyces / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D029702:Schizosaccharomyces pombe Proteins; D016415:Sequence Alignment
C416051:Alg11 protein, S pombe; D005656:Fungal Proteins; D008351:Mannans; D009844:Oligosaccharides; D029702:Schizosaccharomyces pombe Proteins; C012990:galactomannan; D000135:Acid Phosphatase; D005690:Galactose