Nucleotide sequence, genomic organization and cell-cycle-dependent expression of a Chlamydomonas 14-3-3 gene.
Members of the 14-3-3 protein family have been identified as regulatory elements in intracellular signalling pathways and cell cycle control. Previously we reported the nucleotide sequence of a 14-3-3 cDNA cloned from the unicellular green alga Chlamydomonas reinhardtii. In this communication, we describe the nucleotide sequence, the genomic organization and the cell-cycle-dependent expression of the corresponding gene. The coding sequence of this gene was found to be interrupted by four introns of 124, 116, 81, and 659 bp, respectively. Introns 2-4 were found in conserved positions as compared to the Arabidopsis 14-3-3 genes. A counterpart to intron 1 absent in the Arabidopsis 14-3-3 genes was found in the human 14-3-3 epsilon gene.
Voigt
J
J
Physiologisch-chemisches Institut, Universität Tübingen, Hoppe-Seyler-Strasse, Tübingen, Germany. juergen.voigt@uni-tuebingen.de
Liebich
I
I
Wöstemeyer
J
J
Adam
K H
KH
Marquardt
O
O
D048948:14-3-3 Proteins; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002453:Cell Cycle / Q000235:genetics; D002696:Chlamydomonas / Q000235:genetics*; D018076:DNA, Complementary / Q000032:analysis; D016054:DNA, Protozoan / Q000032:analysis; D005091:Exons; D005786:Gene Expression Regulation; D018503:Genome, Protozoan; D007438:Introns; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011506:Proteins / Q000235:genetics*; D015800:Protozoan Proteins / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014446:Tyrosine 3-Monooxygenase
D048948:14-3-3 Proteins; D018076:DNA, Complementary; D016054:DNA, Protozoan; D011506:Proteins; D015800:Protozoan Proteins; D014446:Tyrosine 3-Monooxygenase