Organization and expression of the human zo-2 gene (tjp-2) in normal and neoplastic tissues.
One of the tight junction components, zonula occludens protein 2 (ZO-2), is expressed as two isoforms, ZO-2A and ZO-2C, in normal epithelia. In pancreatic adenocarcinoma of the ductal type ZO-2A is absent, but none of the common mechanisms of gene inactivation is responsible for lack of ZO-2A expression. In the current study, we report the complete organization of the human zo-2 gene (tjp-2), its alternative splicing, and its expression in normal and neoplastic tissues of several organ sites. In addition to pancreatic adenocarcinoma, ZO-2 was found to be de-regulated in breast adenocarcinoma, but not in colon or prostate adenocarcinoma. The latter are considered to be of acinar rather than ductal type. Thus, our data indicate the importance of zo-2 (tjp-2) gene regulation in ductal cancer development and should help to understand the defects of intercellular interactions, critical for suppressing the malignant phenotype.
Chlenski
A
A
Department of Pathology and the Robert H. Lurie Cancer Center, Northwestern University Medical School, Chicago, IL 60611, USA. a-chlenski@nwu.edu
Ketels
K V
KV
Korovaitseva
G I
GI
Talamonti
M S
MS
Oyasu
R
R
Scarpelli
D G
DG
D005091:Exons; D015972:Gene Expression Regulation, Neoplastic; D007438:Introns; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000096:biosynthesis; D014407:Tumor Cells, Cultured; D062827:Zonula Occludens-2 Protein
D008565:Membrane Proteins; D020033:Protein Isoforms; C569046:TJP2 protein, human; D062827:Zonula Occludens-2 Protein