title: Analysis of fission yeast primase defines the checkpoint responses to aberrant S phase initiation.
abstract: To investigate the checkpoint response to aberrant initiation, we analyzed the cell cycle checkpoint response induced by mutations of Schizosaccharomyces pombe DNA primase. DNA primase has two subunits, Spp1 and Spp2 (S. pombe primases 1 and 2). Spp1 is the catalytic subunit that synthesizes the RNA primer, which is then extended by DNA polymerase alpha (Polalpha) to synthesize an initiation DNA structure, and this catalytic function of Polalpha is a prerequisite for generating the S-M phase checkpoint. Here we show that Spp2 is required for coupling the function of Spp1 to Polalpha. Thermosensitive mutations of spp2(+) destabilize the Polalpha-primase complex, resulting in an allele-specific S phase checkpoint defect. The mutant exhibiting a more severe checkpoint defect also has a higher extent of Polalpha-primase complex instability and deficiency in the hydroxyurea-induced Cds1-mediated intra-S phase checkpoint response. However, this mutant is able to activate the Cds1 response to S phase arrest induced by temperature. These findings suggest that the Cds1 response to the S-phase arrest signal(s) induced by a initiation mutant is different from that induced by hydroxyurea. Interestingly, a polalphats mutant with a defective S-M phase checkpoint and an spp2 mutant with an intact checkpoint have a similar Polalpha-primase complex stability, and the Cds1 response induced by hydroxyurea or by the mutant arrests at the restrictive temperature. Thus, the Cds1-mediated intra-S phase checkpoint response induced by hydroxyurea can also be distinguished from the S-M phase checkpoint response that requires the initiation DNA synthesis by Polalpha.
authors.affiliation: Department of Pathology, Stanford University School of Medicine, Stanford, California 94305-5324, USA.
authors.forename: S
authors.initials: S
authors.lastname: Tan
authors.forename: T S
authors.initials: TS
authors.lastname: Wang
mesh_terms: D000483:Alleles; D002455:Cell Division / Q000235:genetics; D002469:Cell Separation; D002470:Cell Survival / Q000187:drug effects; D000071877:Checkpoint Kinase 1; D064447:Checkpoint Kinase 2; D019915:DNA Primase / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D004261:DNA Replication; D018076:DNA, Complementary / Q000378:metabolism; D005434:Flow Cytometry; D006918:Hydroxyurea / Q000494:pharmacology; D008938:Mitosis; D008954:Models, Biological; D016297:Mutagenesis, Site-Directed; D010641:Phenotype; D010957:Plasmids / Q000378:metabolism; D011233:Precipitin Tests; D011494:Protein Kinases / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases; D016196:S Phase; D012568:Schizosaccharomyces / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D029702:Schizosaccharomyces pombe Proteins; D013696:Temperature; D013997:Time Factors; D018631:ras Proteins / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D029702:Schizosaccharomyces pombe Proteins; D011494:Protein Kinases; D064447:Checkpoint Kinase 2; C578636:Cds1 protein, S pombe; D000071877:Checkpoint Kinase 1; D017346:Protein Serine-Threonine Kinases; D019915:DNA Primase; D018631:ras Proteins; D006918:Hydroxyurea