Temperature-dependent vitellogenin-mRNA expression in primary cultures of rainbow trout (Oncorhynchus mykiss) hepatocytes at 14 and 18 degrees C.
In order to study the influence of temperature on vitellogenin gene and estrogen receptor gene expression in primary hepatocytes from rainbow trout (Oncorhynchus mykiss), cells were exposed to 17beta-estradiol, bisphenol-A and nonylphenol for 48 and 96 hr. Induction of vitellogenin-mRNA expression was detected in a non-radioactive dot blot/RNAse protection assay and by RT-PCR. In the dot blot/RNAse protection assay, the estrogenic potentials of bisphenol-A and nonylphenol were about 10(4)- to 10(5)-fold and 10(5)-fold lower than that of 17beta-estradiol, respectively. The relative estrogenic potential did not show any difference between 14 and 18 degrees C. In contrast, at 18 degrees C, RT-PCR analysis revealed increased amounts of vitellogenin- and estrogen receptor-mRNA after 12 and 24 hr of exposure to 17beta-estradiol, if compared to 14 degrees C. Owing to increased vitellogenin gene expression at 18 degrees C, the sensitivity of primary hepatocytes to 17beta-estradiol and bisphenol-A could be increased.
Pawlowski
S
S
Dept of Zoology I, University of Heidelberg, Im Neuenheimer Feld 230, D-69120, Heidelberg, Germany. spawlows@ix.urz.uni-heidelberg.de
Islinger
M
M
Völkl
A
A
Braunbeck
T
T
D000818:Animals; D001559:Benzhydryl Compounds; D002478:Cells, Cultured; D003080:Cold Temperature; D004958:Estradiol / Q000494:pharmacology; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D015151:Immunoblotting; D008297:Male; D023282:Nuclease Protection Assays; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D010636:Phenols / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis*; D011960:Receptors, Estrogen / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014819:Vitellogenins / Q000096:biosynthesis / Q000235:genetics*; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D004958:Estradiol; D010636:Phenols; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D014819:Vitellogenins; C025256:nonylphenol; C006780:bisphenol A; D000098826:Bisphenol A Compounds