Identification of a novel allele, DRB1*1340, in two Brazilian individuals.
We identified a novel HLA-DRB1 allele, named DRB1*1340 by the WHO HLA Nomenclature Committee, in two Brazilian individuals. Typing by polymerase chain reaction using sequence-specific oligonucleotide probes (PCR-SSOP) showed a DRB1*13 allele with an unusual hybridization pattern. DNA sequencing of both strands and comparison of the sequence with previously described DRB1 alleles revealed that the most similar allele is DRB1*1301, from which DRB1*1340 differs by a single nucleotide (T-->A) in exon 2, at position 127, codon 47 (Phe-->Tyr). The sequence received accession number AJ237964 from the EMBL database.
Maciag
P C
PC
Genetics Department, UFPR, Curitiba, Brazil.
Junes
K S
KS
Villa
L L
LL
Petzl-Erler
M L
ML
D000483:Alleles; D001938:Brazil; D006684:HLA-DR Antigens / Q000235:genetics* / Q000276:immunology; D059811:HLA-DRB1 Chains; D006801:Humans; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide / Q000276:immunology; D012689:Sequence Homology, Nucleic Acid
D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains