Do subplate neurons comprise a transient population of cells in developing neocortex of rats?
Studies were undertaken to determine whether neurons of the subplate layer represent a transient or stable population of cells in developing neocortex of rat. The first set of studies sought to determine the fraction of subplate neurons that is lost during early postnatal development. The optical dissector method was used to analyze fluorescently stained material in animals the age of postnatal day 0 (P0) to P40. These results demonstrate a reduction of slightly less than half of the total number of subplate neurons from P0 to P40. Counts of labeled cells in littermates at varied ages after [(3)H]thymidine or BRDU treatment on gestational day 14 (G14 - birthdate of occipital subplate neurons) or G18 (birthdate of layers III-IV neurons) demonstrate loss of approximately 50% of neurons in the subplate layer between P0 and P40, somewhat greater than the loss of neurons from cortical layers III-IV. The second set of studies investigated whether subplate neurons display cellular atrophy during postnatal development. Analysis of subplate neurons injected intracellularly with Lucifer yellow in fixed slice preparations indicates no reduction in soma size, number of dendrites, or extent of dendritic fields of subplate neurons taken from animals age P0 to P60. The third set of studies investigated whether functional markers of subplate neurons are reduced during postnatal development. Analysis of tissue stained histochemically for cytochrome oxidase or acetylcholinesterase, or stained immunocytochemically for GABA, somatostatin, or neuropeptide Y, demonstrate a remarkable loss of expression of staining patterns from late gestational ages to P20. These data demonstrate that, although subplate neurons seem not to be a transient population of cells in the usual sense of being eliminated by cell death or structural atrophy, the loss of histochemical and immunocytochemical markers indicates that they may be a functionally transient population of cells.
Robertson
R T
RT
Department of Anatomy and Neurobiology, College of Medicine, University of California, Irvine, California 92697-1280, USA. rtrobert@uci.edu
Annis
C M
CM
Baratta
J
J
Haraldson
S
S
Ingeman
J
J
Kageyama
G H
GH
Kimm
E
E
Yu
J
J
D000110:Acetylcholinesterase / Q000378:metabolism; D000375:Aging / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D001284:Atrophy; D015415:Biomarkers; D001973:Bromodeoxyuridine; D002470:Cell Survival / Q000502:physiology; D019579:Neocortex / Q000166:cytology* / Q000254:growth & development* / Q000378:metabolism / Q000473:pathology; D009474:Neurons / Q000473:pathology / Q000502:physiology*; D009478:Neuropeptide Y / Q000378:metabolism; D051381:Rats / Q000254:growth & development* / Q000378:metabolism; D013004:Somatostatin / Q000378:metabolism; D013997:Time Factors; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D015415:Biomarkers; D009478:Neuropeptide Y; D013004:Somatostatin; D005680:gamma-Aminobutyric Acid; D000110:Acetylcholinesterase; D001973:Bromodeoxyuridine