Monocyte chemotactic protein 1 upregulates IL-1beta expression in human monocytes.
Monocyte chemotactic protein-1 (MCP-1) chemoattracts and activates monocytes. The nature of the genes that are transcriptionally activated in the monocytes by MCP-1 is not well understood. To identify such genes, human blood monocytes were incubated with or without MCP-1 for periods of 1, 4, and 12 h and the RNA extracted from these monocytes was subjected to differential display. The differentially expressed transcripts were cloned and sequenced. Differential display showed that interleukin-1beta (IL-1beta) gene expression was upregulated by MCP-1 treatment of monocytes for 4 to 12 h. Quantitative PCR and ELISA assays showed that MCP-1 treatment caused elevation in the levels of IL-1beta transcripts and protein, respectively. Immunoblot analysis showed that most of the protein was pro-IL-1beta. Since IL-1beta is known to induce MCP-1 synthesis, the present demonstration that MCP-1 induces IL-1beta synthesis suggests that the induction of each other would amplify the biological effects of these cytokines during inflammation.
Gavrilin
M A
MA
Neurobiotechnology Center and Department of Biochemistry, Ohio State University, 1060 Carmack Road, Columbus, Ohio 43210, USA.
Deucher
M F
MF
Boeckman
F
F
Kolattukudy
P E
PE
D015153:Blotting, Western; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D020869:Gene Expression Profiling; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics* / Q000378:metabolism; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D015854:Up-Regulation / Q000187:drug effects*
D018932:Chemokine CCL2; D007375:Interleukin-1; D011498:Protein Precursors; D012333:RNA, Messenger