Detection of endometrial pinopodes by light microscopy.
OBJECTIVE
To study the value of light microscopy (LM) in the assessment of endometrial pinopodes.


METHODS
Comparative histologic study.


METHODS
Outpatient infertility clinic in an academic teaching institution.


METHODS
Eighteen oocyte donors undergoing controlled ovarian hyperstimulation.


METHODS
Endometrial biopsies on days 14-24 of the cycle.


METHODS
Assessment of pinopodes by scanning electron microscopy (SEM) and of endometrial surface projections by LM.


RESULTS
The luminal surface was identified by LM in 36 of 38 endometrial specimens obtained. Although apical projections could be recognized in all, they were few, moderate, and abundant in 20, 12, and 4 cases, respectively. Pinopodes were detected by SEM in all 4 samples with abundant projections, but in only 14 of 32 samples with lesser quantities of these surface features. No predictive value could be ascribed to apical projections viewed by LM for the developmental stage of pinopodes as defined by SEM.


CONCLUSIONS
The LM of routine endometrial specimens can serve as a preliminary tool in the evaluation of surface morphology. Although abundant apical projections by LM are compatible with the presence of pinopodes by SEM, the latter modality remains as the definitive method in cases with few or moderate projections and for the evaluation of the stage of pinopode development.
Develioglu
O H
OH
The Jones Institute for Reproductive Medicine, Eastern Virginia Medical School, Norfolk, Virginia, USA.
Nikas
G
G
Hsiu
J G
JG
Toner
J P
JP
Jones
H W
HW
D000328:Adult; D000554:Ambulatory Care Facilities; D004717:Endometrium / Q000473:pathology*; D005260:Female; D006801:Humans; D007247:Infertility, Female; D008853:Microscopy; D010062:Ovulation Induction; D013499:Surface Properties; D014019:Tissue Donors