Immune response in chronic periapical parodonititis.
The purpose of our study was to investigate the immune response in chronical periapical parodontitis (CPP) by using multidisciplinary approach. 30 CPP samples were obtained after surgical removal--apicoectomy. Each CPP sample was examined by histological, bacteriological and flow cytometrical (FC) analysis of lymphocytes infiltrating CPP samples. Ten percent of bacteriological samples were sterile, others had significant aerobic and anaerobic growth. We used pathohistologic and microbiologic findings and compared them to the results of immunological analysis. By FC we found a significant increase in proportions of T lymphocytes expressing interleukin-2 receptors and ICAM-1 compared to peripheral blood lymphocytes. Proportions of T helper cells that produce interferon-gama (IFN-gamma) was higher in CPP samples predominantly colonized by anaerobic bacteria. There were no differences in IL-4 expression by T cells in both groups (anaerobic and streptococcal). Among anaerobic CPP samples differences in proportion of T cells that express IL-2 receptors expression was also found between samples colonised by P. acnes and Bacteroides sp. Oral streptococci cause relatively limited tissue destruction and induce Th2 type of immune response accompanied by non-cytotoxic inflammatory reaction. On the contrary, anaerobic bacteria induce Th1 type of immune response that cause more severe inflammatory reaction (type 4) of hypersensitivity that damage the tissue by the action of cytotoxic T cell activation.
Ihan Hren
N
N
Department of Maxilofacial and Oral Surgery, University Clinical Centre Ljubljana, Slovenia.
Gubina
M
M
Kozelj
V
V
Ihan
A
A
D000917:Antibody Formation; D001419:Bacteria / Q000302:isolation & purification; D001421:Bacteria, Anaerobic / Q000302:isolation & purification; D002908:Chronic Disease; D005434:Flow Cytometry; D006099:Granuloma / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D006801:Humans; D016131:Lymphocyte Subsets / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D010483:Periapical Diseases / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D010485:Periapical Periodontitis / Q000276:immunology* / Q000382:microbiology; D013291:Streptococcus / Q000302:isolation & purification