title: Purification, physico-chemical characterization and sequence of a heat labile alkaline metalloprotease isolated from a psychrophilic Pseudomonas species.
abstract: The psychrophilic alkaline metalloprotease (PAP) produced by a Pseudomonas bacterium isolated from Antarctica has been purified and characterized. The gene encoding PAP has been cloned and sequenced and the derived amino acid sequence shows 66% identity with the mesophilic alkaline metalloprotease from Pseudomonas aeruginosa IFO 3455 (AP). Compared to the purified AP, PAP is three times more active at 20 degrees C, is very sensitive to chelating agents and is rapidly inactivated at 45 degrees C. The lower thermostability of PAP can tentatively be explained by a loss of a stabilizing Ca(2+), a decrease in the content of hydrophobic residues and a smaller aliphatic index.
authors.affiliation: Laboratory of Biochemistry, University of Liege, Belgium.
authors.forename: J P
authors.initials: JP
authors.lastname: Chessa
authors.forename: I
authors.initials: I
authors.lastname: Petrescu
authors.forename: M
authors.initials: M
authors.lastname: Bentahir
authors.forename: J
authors.initials: J
authors.lastname: Van Beeumen
authors.forename: C
authors.initials: C
authors.lastname: Gerday
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism; D003001:Cloning, Molecular; D004795:Enzyme Stability; D008666:Metalloendopeptidases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D011549:Pseudomonas / Q000145:classification / Q000201:enzymology* / Q000254:growth & development; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
chemical_list: D011994:Recombinant Proteins; D008666:Metalloendopeptidases; D002118:Calcium