title: Pseudobactin biogenesis in the plant growth-promoting rhizobacterium Pseudomonas strain B10: identification and functional analysis of the L-ornithine N(5)-oxygenase (psbA) gene.
abstract: Pseudobactin(B10), the fluorescent siderophore produced by the rhizobacterium Pseudomonas strain B10, contains the hydroxamate ligand D-N(5)-hydroxyornithine (D-N(5)-OH-Orn). We cloned the L-Orn N(5)-oxygenase (psbA) gene from a genomic library of Pseudomonas strain B10 and demonstrated that PsbA is involved in the conversion of L-Orn to its N(5)-OH derivative. PsbA shows significant similarity to microbial omega-amino acid hydroxylases containing flavin adenine dinucleotide and NADP cofactor-binding sites and the FATGY signature of the putative substrate recognition pocket. The psbA gene is monocistronic, and its transcription is negatively controlled by iron. A site-specific psbA mutant of Pseudomonas strain B10 was biochemically complemented with the precursor L-N(5)-OH-Orn, suggesting that L-Orn is hydroxylated before conversion to the D isomer. The L-Orn N(5)-hydroxylase-defective mutants of Pseudomonas strain B10 and Pseudomonas aeruginosa PAO1 were much less effective than the parental strains in suppressing the growth of the phytopathogen Erwinia carotovora in iron-poor medium. The extent of in vitro inhibition of E. carotovora was strictly iron dependent and directly correlated with the amount of released siderophores. These data strengthen the role of fluorescent siderophores in biocontrol of deleterious rhizomicroorganisms.
authors.affiliation: Istituto di Microbiologia, Università di Roma "La Sapienza", 00100 Rome, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Ambrosi
authors.forename: L
authors.initials: L
authors.lastname: Leoni
authors.forename: L
authors.initials: L
authors.lastname: Putignani
authors.forename: N
authors.initials: N
authors.lastname: Orsi
authors.forename: P
authors.initials: P
authors.lastname: Visca
mesh_terms: D007501:Iron / Q000494:pharmacology; D006899:Mixed Function Oxygenases / Q000235:genetics*; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000378:metabolism*; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics / Q000378:metabolism; D045332:Photosystem II Protein Complex; D017354:Point Mutation; D011549:Pseudomonas / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D017262:Siderophores / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D009842:Oligopeptides; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D017262:Siderophores; C033038:pseudobactin; D007501:Iron; D006899:Mixed Function Oxygenases; C059419:ornithine N5-oxygenase