Inhibition of tumor necrosis factor-alpha-induced apoptosis by Asparagus cochinchinensis in Hep G2 cells.
A human hepatoma cell line, Hep G2 cells, is a reliable system for the study of alcohol-induced hepatotoxicity. In this study, we investigated the effect of an aqueous extract of Asparagus cochinchinensis(MERRIL) (Liliaceae) roots (ACAE) on ethanol (EtOH)-induced cytotoxicity in Hep G2 cells. ACAE (1-100 microg/ml) dose-dependently inhibited the EtOH-induced tumor necrosis factor-alpha (TNF-alpha) secretion. ACAE (1-100 microg/ml) also inhibited the EtOH and TNF-alpha-induced cytotoxicity. Furthermore, we found that ACAE inhibited the TNF-alpha-induced apoptosis of Hep G2 cells. These results suggest that ACAE may prevent the EtOH-induced cytotoxicity through inhibition of the apoptosis of Hep G2 cells.
Koo
H N
HN
College of Pharmacy, Wonkwang University School of Medicine, Center of Oriental Medicinal Science, Wonkwang University, Iksan, 570-749, Chonbuk, South Korea.
Jeong
H J
HJ
Choi
J Y
JY
Choi
S D
SD
Choi
T J
TJ
Cheon
Y S
YS
Kim
K S
KS
Kang
B K
BK
Park
S T
ST
Chang
C H
CH
Kim
C H
CH
Lee
Y M
YM
Kim
H M
HM
An
N H
NH
Kim
J J
JJ
D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D000431:Ethanol / Q000037:antagonists & inhibitors / Q000633:toxicity*; D006801:Humans; D019668:Liliaceae; D008113:Liver Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D010936:Plant Extracts / Q000627:therapeutic use*; D018517:Plant Roots; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000378:metabolism
D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D000431:Ethanol