[Analysis of natural medicines by micellar electrokinetic chromatography (MEKC)--determination of calycosin in Astragali radix].
We previously reported a HPLC identification method for Astragali Radix and its fluid extract using calycosin as a marker substance. However, it took about 40 min for one run using gradient elution. Therefore, we alternatively employed capillary electrophoresis (CE) for the analysis of calycosin in those materials. As a result, calycosin was clearly separated from the other components in Astragali Radix and its fluid extract by micellar electrokinetic chromatography (MEKC) mode with sodium dodecyl sulfate (SDS) within 5 min. Then we determined the content of calycosin in a drinkable preparation. Validation for the developed method was also performed in accordance with the ICH guideline.
Kuwahara
Y
Y
Analytical Chemistry Department, Tanabe Seiyaku Co., Ltd., Osaka, Japan.
Nakamura
T
T
Hashimoto
A
A
Nishi
H
H
D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D007529:Isoflavones / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*
D007529:Isoflavones; D010936:Plant Extracts; C121707:7,3'-dihydroxy-4'-methoxyisoflavone