Determination of zopiclone in tablets by HPLC and UV-spectrophotometry.
Rapid, simple and accurate chromatographic (HPLC) and spectrophotometric methods for the determination of zopiclone in tablets were elaborated. Acetonitrile was found to be a suitable extraction solvent. The samples were chromatographed on Nova-Pak C18 column and UV detection at 304 nm. The elution was achieved isocratically with a mobile phase of 0.067 M phosphate buffer pH 7.95 - acetonitrile (55:45, v/v). Diazepam was applied as an internal standard. The method was validated for precision, linearity, accuracy and limit of detection. The recovery (mean +/- SD) in HPLC was 99.85 + 0.04% and in the UV-spectrophotometry 100.08 +/- 0.09%.
Paw
B
B
Department of Medicinal Chemistry, Faculty of Pharmacy, Medical Academy, Lublin, Poland.
Misztal
G
G
D053961:Azabicyclo Compounds; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D006993:Hypnotics and Sedatives / Q000032:analysis*; D007202:Indicators and Reagents; D010879:Piperazines / Q000032:analysis*; D015203:Reproducibility of Results; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D013607:Tablets / Q000032:analysis
D053961:Azabicyclo Compounds; D006993:Hypnotics and Sedatives; D007202:Indicators and Reagents; D010879:Piperazines; D012996:Solutions; D013607:Tablets; C515050:zopiclone