Serological and nucleotide sequence analysis of HLA-A*6812.
We describe a novel HLA-A allele, HLA-A*6812. HLA-typing of a patient was performed by serology and by DNA-based analyses. Cloning and sequencing of exons 2 and 3 revealed that the genotype consisted of a common HLA-A*0101 allele and the novel HLA-A*6812 allele. HLA*6812 is identical to HLA-A*68012 except for a single non-synonymous nucleotide substitution leading to an exchange of a threonine to an isoleucine residue at position 142 of the HLA-class I alpha chain. The allele was also found in the patient's mother. The novel HLA-A68 molecule is recognised by some but not all of our HLA-A28-specific sera. These results confirm that position 142 is important for the serological properties of the HLA-A molecule.
Faé
I
I
Department of Blood Group Serology, University of Vienna, Austria.
Gleissner
B
B
Kriks
D
D
Leitner
D
D
Fischer
G F
GF
D000483:Alleles; D001483:Base Sequence; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology; D005260:Female; D015234:HLA-A Antigens / Q000235:genetics* / Q000276:immunology*; D006650:Histocompatibility Testing; D006801:Humans; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
D000939:Epitopes; D015234:HLA-A Antigens; C420208:HLA-A*68:12 antigen; C037164:HLA-A28 antigen