Phenylacetyl-coenzyme A is the true inducer of the phenylacetic acid catabolism pathway in Pseudomonas putida U.
Aerobic degradation of phenylacetic acid in Pseudomonas putida U is carried out by a central catabolism pathway (phenylacetyl-coenzyme A [CoA] catabolon core). Induction of this route was analyzed by using different mutants specifically designed for this objective. Our results revealed that the true inducer molecule is phenylacetyl-CoA and not other structurally or catabolically related aromatic compounds.
García
B
B
Departamento de Bioquímica y Biología Molecular, Facultad de Veterinaria, Campus de Vegazana s/n, 24007 León, Spain.
Olivera
E R
ER
Miñambres
B
B
Carnicero
D
D
Muñiz
C
C
Naharro
G
G
Luengo
J M
JM
D000105:Acetyl Coenzyme A / Q000378:metabolism*; D000332:Aerobiosis; D002250:Carbon Radioisotopes; D005798:Genes, Bacterial; D005809:Genes, Regulator; D008956:Models, Chemical; D010648:Phenylacetates / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D016958:Pseudomonas putida / Q000235:genetics / Q000378:metabolism*
D002250:Carbon Radioisotopes; D010648:Phenylacetates; D000105:Acetyl Coenzyme A; C037816:phenylacetyl-coenzyme A; C025136:phenylacetic acid