title: Molecular cloning and expression of rat betacellulin cDNA.
abstract: The cDNA encoding an entire open reading frame of rat betacellulin has been cloned from rat kidney. Expression of this cDNA in COS7 cells showed a significant amount of mitogenic activity in the culture media. Western blotting of the cell lysates suggested that the membrane-anchored precursor was cleaved to release its ectodomain very efficiently.
authors.affiliation: Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, Tsushima-naka, Okayama, Japan. tadahrk@biotech.okayama-u.ac.jp
authors.forename: H
authors.initials: H
authors.lastname: Tada
authors.forename: M
authors.initials: M
authors.lastname: Seno
authors.forename: H
authors.initials: H
authors.lastname: Yamada
authors.forename: R
authors.initials: R
authors.lastname: Sasada
authors.forename: K
authors.initials: K
authors.lastname: Igarashi
mesh_terms: D016475:3T3 Cells / Q000187:drug effects; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D066259:Betacellulin; D019556:COS Cells; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis; D004815:Epidermal Growth Factor / Q000737:chemistry; D015870:Gene Expression; D006133:Growth Substances / Q000235:genetics* / Q000494:pharmacology; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D007668:Kidney / Q000502:physiology; D051379:Mice; D008934:Mitogens / Q000494:pharmacology; D008969:Molecular Sequence Data; D051381:Rats; D017386:Sequence Homology, Amino Acid; D014407:Tumor Cells, Cultured
chemical_list: C587851:BTC protein, human; D066259:Betacellulin; C587852:Btc protein, mouse; C587853:Btc protein, rat; D018076:DNA, Complementary; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D008934:Mitogens; D004815:Epidermal Growth Factor