title: An improved genetic linkage map of rat chromosome 20.
abstract: OBJECTIVE
  Rat chromosome 20 is one of special interest because it contains some diabetogenic genes, such as a major histocompatibilitiy complex (MHC)-linked genetic components and quantitative trait loci. We studied rat chromosome 20, using the backcross progeny between BB/Wor and PVG.R23 rats, and confirmed the genetic linkage map by use of another backcross panel.
  
  
  METHODS
  Backcross panels were done between BB/Wor and PVG.R23 rats, and BN and KZC rats. Length variations of simple sequence length polymorphism markers were analyzed by use of polymerase chain reaction (PCR) analysis. Alleles of RT1-Bb and RT1-Db were analyzed by use of the PCR-restriction fragment length polymorphism method. Genetic maps of rat chromosome 20 were constructed, using the Map Manager computer program.
  
  
  RESULTS
  Fifty-two loci were mapped on rat chromosome 20. Genetic length was 57.9 cM, with average spanning of 1.11 cM between markers. The positions of RT1-N1, Tnf, and RT1-Bb into the MHC region were separated and confirmed by results of two backcross panels in our linkage studies.
  
  
  CONCLUSIONS
  The genetic linkage map of rat chromosome 20 was improved, and was a useful tool for genetic analysis of a diabetogenic gene(s) and for producing MHC congenic strains.
authors.affiliation: Research Laboratories, Torii Pharmaceutical Co Ltd, Chiba, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Masuyama
authors.forename: J
authors.initials: J
authors.lastname: Ishibiki
authors.forename: T
authors.initials: T
authors.lastname: Awata
authors.forename: M
authors.initials: M
authors.lastname: Noda
authors.forename: Y
authors.initials: Y
authors.lastname: Kanazawa
authors.forename: M
authors.initials: M
authors.lastname: Sugawara
authors.forename: K
authors.initials: K
authors.lastname: Komeda
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D008040:Genetic Linkage; D005819:Genetic Markers; D051379:Mice; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D051381:Rats; D011913:Rats, Inbred BB / Q000235:genetics; D011914:Rats, Inbred BN / Q000235:genetics; D011919:Rats, Inbred Strains / Q000235:genetics*; D012984:Software
chemical_list: D005819:Genetic Markers