Alternative splicing of RHAMM gene in chinese gastric cancers and its in vitro regulation.
OBJECTIVE
To identify the receptor of hyaluronic acid mediated motility (RHAMM), expression pattern(s) in the gastric mucosa with various lesions and to elucidate the effect of hyaluronic acid(HA) on in vitro RHAMM expression.


METHODS
Immunocytochemical staining and Western blot hybridization were performed to check the expression patterns and locations of RHAMM molecules in the noncancerous, preneoplastic and malignant gastric specimens. RHAMM expression of the two stomach cancer cell lines cultured conventionally or under the conditions with gelatin or HA supplementation was evaluated as well.


RESULTS
Mr 93000-95000 intracellular RHAMM, so-called IHABP, proteins were detected in 17% noncancerous mucosa. The multiple spectrums and increased frequencies of RHAMM expression could be observed in the majority of the cancer cases with both intracellular and cell surface labeling. HA but not gelatin could promote membrane type RHAMM (mRHAMM) expression of stomach cancer cells in a dose associated pattern.


CONCLUSIONS
The expression of mRHAMM variants is closely associated with tumor progression, which may be promoted and modulated by HA.
Li
H
H
Division of Molecular Biology, Shenyang Medical College, Shenyang, Liaoning 110031 P.R.China. lihongm@pub.sy.ln.net.cn
Guo
L
L
Li
J
J
Liu
N
N
Liu
J
J
D017398:Alternative Splicing; D016326:Extracellular Matrix Proteins / Q000235:genetics*; D005753:Gastric Mucosa / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D018960:Hyaluronan Receptors / Q000235:genetics*; D006820:Hyaluronic Acid / Q000494:pharmacology*; D013274:Stomach Neoplasms / Q000235:genetics*
D016326:Extracellular Matrix Proteins; D018960:Hyaluronan Receptors; C098946:hyaluronan-mediated motility receptor; D006820:Hyaluronic Acid