title: Linear extrapolation method of analyzing solvent denaturation curves.
abstract: The two most common methods of measuring the conformational stability of a protein are differential scanning calorimetry and an analysis of solvent denaturation curves by using the linear extrapolation method. In this article, we trace the history of the linear extrapolation method, review how the method is used to measure protein stability, and then discuss some of the other important uses.
authors.affiliation: Departments of Medical Biochemistry and Genetics, Biochemistry & Biophysics, and Center for Macromolecular Design, Texas A&M University, College Station, Texas 77843-1114, USA. nickpace@tamu.edu
authors.forename: C N
authors.initials: CN
authors.lastname: Pace
authors.forename: K L
authors.initials: KL
authors.lastname: Shaw
mesh_terms: D002152:Calorimetry, Differential Scanning; D011489:Protein Denaturation; D012997:Solvents; D013816:Thermodynamics
chemical_list: D012997:Solvents