title: Expression of mRNAs encoding for alpha and beta integrin subunits, MMPs, and TIMPs in stretched human periodontal ligament and gingival fibroblasts.
abstract: The biological mechanisms of tooth movement result from the cellular responses of connective tissues to exogenous mechanical forces. Among these responses, the degradation of the extracellular matrix takes place, but the identification of the molecular basis as well as the components implicated in this degradation are poorly understood. To contribute to this identification, we subjected human fibroblasts obtained from the periodontal ligament (PDLs) and from the gingiva (HGFs) to a continuous stretch to quantify the mRNAs encoding for various metalloproteinases (MMPs), their tissue inhibitors (TIMPs), and alpha and beta integrin subunits. Both cell lines reacted by inducing the expression of the mRNAs encoding for MMP-1, MMP-2, TIMP-1, and TIMP-2, while other mRNAs did not vary (MT1-MMP, TIMP-3) or were not expressed (MMP-9). PDLs expressed selectively the mRNAs encoding for alpha4 and alphav, with no difference measurable under stretching, while the mRNAs encoding for alpha6 and beta1 were increased and the one encoding for alpha5 was decreased. HGFs increased the mRNAs encoding for alpha2, alpha6, beta1, and beta3 and decreased the one encoding for alpha3. Analysis of our data indicated that stretched HGFs and PDLs induced the same pattern of mRNAs encoding for MMPs and TIMPs but differed for those encoding various integrin subunits, known to act as protein receptors in mechanotransduction.
authors.affiliation: Unité INSERM U424, Fédération de Recherches Odontologiques, Strasbourg, France.
authors.forename: A L
authors.initials: AL
authors.lastname: Bolcato-Bellemin
authors.forename: R
authors.initials: R
authors.lastname: Elkaim
authors.forename: A
authors.initials: A
authors.lastname: Abehsera
authors.forename: J L
authors.initials: JL
authors.lastname: Fausser
authors.forename: Y
authors.initials: Y
authors.lastname: Haikel
authors.forename: H
authors.initials: H
authors.lastname: Tenenbaum
mesh_terms: D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology*; D005881:Gingiva / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D016023:Integrins / Q000378:metabolism*; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D010513:Periodontal Ligament / Q000378:metabolism* / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods / Q000706:statistics & numerical data; D018709:Statistics, Nonparametric; D013314:Stress, Mechanical; D019714:Tissue Inhibitor of Metalloproteinases / Q000378:metabolism*; D014086:Tooth Mobility / Q000235:genetics / Q000378:metabolism
chemical_list: D016023:Integrins; D012333:RNA, Messenger; D019714:Tissue Inhibitor of Metalloproteinases; D020782:Matrix Metalloproteinases