title: Expression of endothelial nitric oxide synthase in human peritoneal tissue: regulation by Escherichia coli lipopolysaccharide.
abstract: Changes in the expression of endothelial nitric oxide synthase (eNOS) in the peritoneum could be involved in the peritoneal dysfunction associated with peritoneal inflammation. Demonstrated recently in bovine endothelial cells was the existence of cytosolic proteins that bind to the 3'-untranslated region (3'-UTR) of eNOS mRNA and could be implicated in eNOS mRNA stabilization. The present work demonstrates that eNOS protein is expressed in human endothelial and mesothelial peritoneal cells. Escherichia coli lipopolysaccharide shortened the half-life of eNOS message, reducing eNOS protein expression in peritoneal mesothelial and endothelial cells. Moreover, under basal conditions, human peritoneal samples expressed cytosolic proteins that bind to the 3'-UTR of eNOS mRNA. The cytosolic proteins that directly bind to 3'-UTR were identified as a 60-kD protein. After incubation of human peritoneal samples with lipopolysaccharide, the binding activity of the cytosolic 60-kD protein increased in a time-dependent manner. Studies are now necessary to determine the involvement of this 60-kD protein in the regulation of eNOS expression in peritoneal cells and particularly its involvement in the peritoneal dysfunction associated with inflammatory reactions.
authors.affiliation: Cardiovascular Research and Hypertension Laboratory, Fundación Jiménez Díaz, Madrid, Spain.
authors.forename: María M
authors.initials: MM
authors.lastname: Arriero
authors.affiliation: Cardiovascular Research and Hypertension Laboratory, Fundación Jiménez Díaz, Madrid, Spain.
authors.forename: Juan A
authors.initials: JA
authors.lastname: Rodríguez-Feo
authors.affiliation: Cardiovascular Research and Hypertension Laboratory, Fundación Jiménez Díaz, Madrid, Spain.
authors.forename: Ángel
authors.initials: Á
authors.lastname: Celdrán
authors.affiliation: Cardiovascular Research and Hypertension Laboratory, Fundación Jiménez Díaz, Madrid, Spain.
authors.forename: Lourdes Sánchez DE
authors.initials: LS
authors.lastname: Miguel
authors.affiliation: Cardiovascular Research and Hypertension Laboratory, Fundación Jiménez Díaz, Madrid, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: González-Fernández
authors.affiliation: Department of Pathology, Fundación, Jiménez Díaz, Madrid, Spain.
authors.forename: José
authors.initials: J
authors.lastname: Fortes
authors.affiliation: Cardiovascular Research and Hypertension Laboratory, Fundación Jiménez Díaz, Madrid, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Reyero
authors.affiliation: Cardiovascular Research and Hypertension Laboratory, Fundación Jiménez Díaz, Madrid, Spain.
authors.forename: Octavio
authors.initials: O
authors.lastname: Frieyro
authors.affiliation: Cardiovascular Research and Hypertension Laboratory, Fundación Jiménez Díaz, Madrid, Spain.
authors.forename: Juan C DE LA
authors.initials: JC
authors.lastname: Pinta
authors.affiliation: Cardiovascular Research and Hypertension Laboratory, Fundación Jiménez Díaz, Madrid, Spain.
authors.forename: Angeles
authors.initials: A
authors.lastname: Franco
authors.affiliation: Cardiovascular Research and Hypertension Laboratory, Fundación Jiménez Díaz, Madrid, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Pastor
authors.affiliation: Cardiovascular Research and Hypertension Laboratory, Fundación Jiménez Díaz, Madrid, Spain.
authors.forename: Santos
authors.initials: S
authors.lastname: Casado
authors.affiliation: Cardiovascular Research and Hypertension Laboratory, Fundación Jiménez Díaz, Madrid, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: López-Farré
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D003600:Cytosol / Q000378:metabolism; D004926:Escherichia coli; D006801:Humans; D007158:Immunologic Techniques; D008070:Lipopolysaccharides / Q000494:pharmacology*; D019001:Nitric Oxide Synthase / Q000235:genetics / Q000378:metabolism*; D052250:Nitric Oxide Synthase Type III; D010537:Peritoneum / Q000201:enzymology*; D011506:Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology
chemical_list: D020413:3' Untranslated Regions; D008070:Lipopolysaccharides; D011506:Proteins; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; C496314:NOS3 protein, human; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III