title: Cementum attachment protein enriches putative cementoblastic populations on root surfaces in vitro.
abstract: We tested the capacity of cementum attachment protein (CAP) to recruit putative cementoblastic populations to root surfaces in vitro by determining the phenotypic expression of periodontal ligament cloned cell populations. The clones were derived from cells that attached to either CAP-coated (experimental) or uncoated (control) root slices. Root slices were co-cultured with primary human periodontal ligament cells. Cloned and parent populations were analyzed for their capacity to express alkaline phosphatase (AP), osteopontin, bone sialoprotein (BSP), and CAP and to form mineralized tissue in vitro. The percentage of CAP- and BSP-positive clones was significantly higher in the experimental clones than in the controls. The percentage of cells positive for AP, BSP, and CAP was higher in the experimental clones than in their control counterparts. Mineralized tissue formation was observed only in the cell populations derived from the CAP-coated root slices. These results indicate that CAP is capable of recruiting putative cementoblastic populations on root slices in vitro and therefore might play an important role in cementogenesis during periodontal homeostasis and wound healing.
authors.affiliation: Department of Oral Biology, Goldschleger School of Dental Medicine, Tel Aviv University, Israel.
authors.forename: I
authors.initials: I
authors.lastname: BarKana
authors.forename: A S
authors.initials: AS
authors.lastname: Narayanan
authors.forename: A
authors.initials: A
authors.lastname: Grosskop
authors.forename: N
authors.initials: N
authors.lastname: Savion
authors.forename: S
authors.initials: S
authors.lastname: Pitaru
mesh_terms: D000469:Alkaline Phosphatase / Q000096:biosynthesis; D000704:Analysis of Variance; D000818:Animals; D002417:Cattle; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000502:physiology*; D002465:Cell Movement; D025021:Cementogenesis; D002999:Clone Cells / Q000378:metabolism; D003739:Dental Cementum / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D058574:Integrin-Binding Sialoprotein; D009805:Odontogenesis / Q000502:physiology*; D053495:Osteopontin; D010513:Periodontal Ligament / Q000166:cytology* / Q000378:metabolism; D011485:Protein Binding; D012795:Sialoglycoproteins / Q000096:biosynthesis; D014074:Tooth Calcification / Q000502:physiology; D014092:Tooth Root / Q000166:cytology / Q000378:metabolism
chemical_list: D015815:Cell Adhesion Molecules; C546871:IBSP protein, human; D058574:Integrin-Binding Sialoprotein; C506895:SPP1 protein, human; D012795:Sialoglycoproteins; C433705:cementum attachment protein, 55-kDa; D053495:Osteopontin; D000469:Alkaline Phosphatase