title: The dentinal structure of equine incisors: a light and scanning electron-microscopic study.
abstract: This paper gives an anatomical overview of the dentinal structure in equine incisor teeth with special reference to the three-dimensional organization, the number and the diameter of the dentinal tubules. The spatial arrangement of equine dentine was examined by scanning electron microscopy of occlusal surfaces and longitudinally fractured teeth and by light microscopy of both decalcified and ground sections. The dentinal tubules of the peripherally situated primary dentine were directly continuous with those of the circumpulpal secondary dentine. The tubules had numerous side branches along their entire course and ramified into terminal branches near the dentino-enamel junction. Tubules of tertiary dentine, situated in the centre of the dental star, were few in number and not continuous with those of the surrounding secondary dentine. On non-etched incisors tubular orifices were clogged by an amorphous smear layer that covered the occlusal surface. On etched occlusal surfaces the numerical tubular density was calculated in different zones. The largest number of tubules per unit area was situated in the secondary dentine forming the dark periphery of the dental star, whereas the smallest number was present in the tertiary dentine located in the pale centre of the dental star. Dentinal tubular diameters were measured at various distances from the pulp. They were widest near the pulpal wall and narrowed progressively towards the dentino-enamel junction. Tubular widths measured on etched occlusal surfaces were considerably larger than those measured on undecalcified incisors due to the removal of intratubular dentine during the etching process.
authors.affiliation: Department of Morphology, Faculty of Veterinary Medicine, Merelbeke, Belgium. sofie.muylle@rug.ac.be
authors.forename: S
authors.initials: S
authors.lastname: Muylle
authors.forename: P
authors.initials: P
authors.lastname: Simoens
authors.forename: H
authors.initials: H
authors.lastname: Lauwers
mesh_terms: D000818:Animals; D003804:Dentin / Q000033:anatomy & histology* / Q000648:ultrastructure; D006736:Horses / Q000033:anatomy & histology*; D007180:Incisor / Q000033:anatomy & histology* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning