title: Microscale diversity of the genus Nitrobacter in soil on the basis of analysis of genes encoding rRNA.
abstract: We looked at the diversity of [NO(2)](-) oxidizers at field scale by examining isolates at clump scale and in microsamples of soil (diameter, 50 microm). The genetic distances (as determined by amplified ribosomal DNA restriction analysis performed with Nitrobacter-specific primers) in a small clump of soil were as large as those between reference strains from large geographical areas. Diversity in individual microsamples was shown by serotyping.
authors.affiliation: Laboratoire d'Ecologie Microbienne, UMR 5557 Université Claude Bernard, 69622 Villeurbanne Cedex, France. grundman@biomserv.univ-lyon1.fr
authors.forename: G L
authors.initials: GL
authors.lastname: Grundmann
authors.forename: P
authors.initials: P
authors.lastname: Normand
mesh_terms: D005075:Biological Evolution; D017931:DNA Primers; D004275:DNA, Ribosomal / Q000235:genetics*; D005798:Genes, Bacterial; D014644:Genetic Variation; D005838:Genotype; D009566:Nitrates / Q000378:metabolism; D009576:Nitrobacter / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D010084:Oxidation-Reduction; D010802:Phylogeny; D015183:Restriction Mapping; D012988:Soil Microbiology
chemical_list: D017931:DNA Primers; D004275:DNA, Ribosomal; D009566:Nitrates