title: Structural proteomics of an archaeon.
abstract: A set of 424 nonmembrane proteins from Methanobacterium thermoautotrophicum were cloned, expressed and purified for structural studies. Of these, approximately 20% were found to be suitable candidates for X-ray crystallographic or NMR spectroscopic analysis without further optimization of conditions, providing an estimate of the number of the most accessible structural targets in the proteome. A retrospective analysis of the experimental behavior of these proteins suggested some simple relations between sequence and solubility, implying that data bases of protein properties will be useful in optimizing high throughput strategies. Of the first 10 structures determined, several provided clues to biochemical functions that were not detectable from sequence analysis, and in many cases these putative functions could be readily confirmed by biochemical methods. This demonstrates that structural proteomics is feasible and can play a central role in functional genomics.
authors.affiliation: Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto 610 University Ave, Toronto, Ontario, Canada M5G 2M9.
authors.forename: D
authors.initials: D
authors.lastname: Christendat
authors.forename: A
authors.initials: A
authors.lastname: Yee
authors.forename: A
authors.initials: A
authors.lastname: Dharamsi
authors.forename: Y
authors.initials: Y
authors.lastname: Kluger
authors.forename: A
authors.initials: A
authors.lastname: Savchenko
authors.forename: J R
authors.initials: JR
authors.lastname: Cort
authors.forename: V
authors.initials: V
authors.lastname: Booth
authors.forename: C D
authors.initials: CD
authors.lastname: Mackereth
authors.forename: V
authors.initials: V
authors.lastname: Saridakis
authors.forename: I
authors.initials: I
authors.lastname: Ekiel
authors.forename: G
authors.initials: G
authors.lastname: Kozlov
authors.forename: K L
authors.initials: KL
authors.lastname: Maxwell
authors.forename: N
authors.initials: N
authors.lastname: Wu
authors.forename: L P
authors.initials: LP
authors.lastname: McIntosh
authors.forename: K
authors.initials: K
authors.lastname: Gehring
authors.forename: M A
authors.initials: MA
authors.lastname: Kennedy
authors.forename: A R
authors.initials: AR
authors.lastname: Davidson
authors.forename: E F
authors.initials: EF
authors.lastname: Pai
authors.forename: M
authors.initials: M
authors.lastname: Gerstein
authors.forename: A M
authors.initials: AM
authors.lastname: Edwards
authors.forename: C H
authors.initials: CH
authors.lastname: Arrowsmith
mesh_terms: D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D017014:Methanobacterium / Q000235:genetics / Q000378:metabolism*; D011487:Protein Conformation; D020543:Proteome
chemical_list: D020543:Proteome