p55CDC/hCDC20 is associated with BUBR1 and may be a downstream target of the spindle checkpoint kinase.
Eukaryotic cells have evolved a mechanism that delays the progression of mitosis until condensed chromosomes are properly positioned on the mitotic spindle. We have been studying genes that regulated the spindle checkpoint in human cells. Enforced expression of human BUBR1, but not a BUBR1 mutant allele, enhances accumulation of mitotic cells. Yeast two-hybrid system and GST-pulldown analyses show that p55CDC/hCdc20, a protein known to link spindle checkpoint components such as MAD2 to anaphase promoting complex (APC), interacts with BUBR1. In addition, p55CDC is capable of pulling down BUBR1 in sf-9 cells infected with both p55CDC and His6-BUBR1 recombinant baculoviruses but not in the cells infected with p55CDC baculoviruses or with the baculoviral vector alone. Moreover, immunoprecipitation followed by Western blot analyses confirmed that native p55CDC is associated with BUBR1 in HeLa cells. Spindle checkpoint activation by nocodazole treatment enhances the association between p55CDC and His6-BUBR1. In nocodazole-arrested mitotic cells, both CDC16 and hyperphosphorylated CDC27, two APC components, preferentially associate with His6-BUBR1 resins, but not the control resins. Furthermore, BUBR1 phosphorylates p55CDC in vitro, and the phosphorylation of p55CDC by BUBR1 appears to be correlated with spindle checkpoint activation. Together, our studies strongly suggest that BUBR1 may target APC via p55CDC.
Wu
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American Health Foundation, Valhalla, New York, NY 10595, USA.
Lan
Z
Z
Li
W
W
Wu
S
S
Weinstein
J
J
Sakamoto
K M
KM
Dai
W
W
D000483:Alleles; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002352:Carrier Proteins; D064199:Cdc20 Proteins; D018797:Cell Cycle Proteins; D018076:DNA, Complementary / Q000235:genetics; D005656:Fungal Proteins / Q000378:metabolism; D017930:Genes, Reporter; D018816:Genes, cdc; D006367:HeLa Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D046911:Macromolecular Substances; D008938:Mitosis / Q000502:physiology*; D015739:Nocodazole / Q000494:pharmacology; D009687:Nuclear Proteins; D017354:Point Mutation; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases; D011506:Proteins / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000502:physiology; D008941:Spindle Apparatus / Q000187:drug effects / Q000378:metabolism*; D014162:Transfection; D020798:Two-Hybrid System Techniques
D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D064199:Cdc20 Proteins; D018797:Cell Cycle Proteins; D018076:DNA, Complementary; D005656:Fungal Proteins; D046911:Macromolecular Substances; D009687:Nuclear Proteins; D011506:Proteins; D011993:Recombinant Fusion Proteins; C577488:CDC20 protein, human; D011494:Protein Kinases; C584277:BUB1 protein, human; C090464:Bub1 spindle checkpoint protein; D017346:Protein Serine-Threonine Kinases; D015739:Nocodazole