title: Transport of mitochondria during axonogenesis.
abstract: The cellular mechanisms involved in axonogenesis are still unclear. In the present work we found that formation of neurites in cultured neonatal dorsal root ganglion neurons co-incided with the redistribution of highly charged mitochondria. Radially distributed in subplasmalemmal space 3 h after plating, highly charged mitochondria formed clusters in the hillocks of predominant neurites during the next 2448 h and then redistributed into the axons. These results provide evidence that accumulation of a critical mass of charged mitochondria at the site of the future axonal hillock may represent the slow initiation stage of axonogenesis, followed by a fast growth phase.
authors.affiliation: Department of Pharmacy, School of Biological Sciences, University of Sydney, NSW, Australia. vadimd@pharm.usyd.edu.au
authors.forename: V N
authors.initials: VN
authors.lastname: Dedov
authors.forename: I V
authors.initials: IV
authors.lastname: Dedova
authors.forename: P J
authors.initials: PJ
authors.lastname: Armati
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology* / Q000648:ultrastructure; D001693:Biological Transport, Active; D002478:Cells, Cultured; D005727:Ganglia, Spinal / Q000166:cytology; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000502:physiology* / Q000648:ultrastructure; D009068:Movement; D016501:Neurites / Q000502:physiology / Q000648:ultrastructure; D009474:Neurons / Q000502:physiology / Q000648:ultrastructure; D051381:Rats