title: Reversal of nitric oxide-, peroxynitrite- and S-nitrosothiol-induced inhibition of mitochondrial respiration or complex I activity by light and thiols.
abstract: Nitric oxide (NO) and its derivatives peroxynitrite and S-nitrosothiols inhibit mitochondrial respiration by various means, but the mechanisms and/or the reversibility of such inhibitions are not clear. We find that the NO-induced inhibition of respiration in isolated mitochondria due to inhibition of cytochrome oxidase is acutely reversible by light. Light also acutely reversed the inhibition of respiration within iNOS-expressing macrophages, and this reversal was partly due to light-induced breakdown of NO, and partly due to reversal of the NO-induced inhibition of cytochrome oxidase. NO did not cause inhibition of complex I activity within isolated mitochondria, but 0.34 mM peroxynitrite, 1 mM S-nitroso-N-acetylpenicillamine or 1 mM S-nitrosoglutathione did cause substantial inhibition of complex I activity. Inhibition by these reagents was reversed by light, dithiothreitol or glutathione-ethyl ester, either partially or completely, depending on the reagent used. The rapid inhibition of complex I activity by S-nitroso-N-acetylpenicillamine also occurred in conditions where there was little or no release of free NO, suggesting that the inhibition was due to transnitrosylation of the complex. These findings have implications for the physiological and pathological regulation of respiration by NO and its derivatives.
authors.affiliation: Dpartment of Biochemistry, University of Cambridge, UK.
authors.forename: V
authors.initials: V
authors.lastname: Borutaite
authors.forename: A
authors.initials: A
authors.lastname: Budriunaite
authors.forename: G C
authors.initials: GC
authors.lastname: Brown
mesh_terms: D000818:Animals; D002460:Cell Line; D019069:Cell Respiration / Q000187:drug effects; D042967:Electron Transport Complex I; D004734:Energy Metabolism; D004789:Enzyme Activation; D008027:Light; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology; D008623:Mercaptoethanol; D051379:Mice; D008929:Mitochondria, Heart / Q000187:drug effects* / Q000201:enzymology; D009247:NADH, NADPH Oxidoreductases / Q000032:analysis / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009566:Nitrates / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D009603:Nitroso Compounds / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D010101:Oxygen Consumption; D051381:Rats; D026403:S-Nitrosothiols; D013438:Sulfhydryl Compounds / Q000494:pharmacology*
chemical_list: D009566:Nitrates; D009603:Nitroso Compounds; D026403:S-Nitrosothiols; D013438:Sulfhydryl Compounds; C039642:peroxynitric acid; D009569:Nitric Oxide; D008623:Mercaptoethanol; C032635:S-nitrosomercaptoethanol; D009247:NADH, NADPH Oxidoreductases; D042967:Electron Transport Complex I