Monocytes are target cells for IL-10 induction by HIV-1 Nef protein.
IL-10 plays a pivotal role in the pathogenesis of several diseases and is elevated in sera of HIV-infected patients. Recently, we demonstrated that HIV Nef induces IL-10 mRNA expression as well as IL-10 production using PBMCs, H9 or U937 cells. This induction of IL-10 is inhibited by a calmodulin antagonist, W-7. In the present study, T or B lymphocytes or monocytes were isolated from PBMCs of healthy HIV-negative donors. Production of IL-10 and mRNA gene expression were analyzed on each isolated cell population after treatment with Nef or SEA for 3-24 h. The results show that Nef induces IL-10 production as well as mRNA expression significantly using monocytes but not with T or B lymphocytes. By contrast, SEA induced IL-10 production as well as mRNA expression using T lymphocytes but not with monocytes or B lymphocytes.
Tangsinmankong
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Department of Pediatrics, University of South Florida College of Medicine, All Children's Hospital, St. Petersburg, FL 33701, USA.
Day
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Good
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Haraguchi
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D030161:Avian Proteins; D001402:B-Lymphocytes / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D015693:Gene Products, nef / Q000096:biosynthesis* / Q000494:pharmacology; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis*; D007700:Kinetics; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D009000:Monocytes / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000378:metabolism; D013997:Time Factors
D030161:Avian Proteins; D015693:Gene Products, nef; D012333:RNA, Messenger; D011994:Recombinant Proteins; D016753:Interleukin-10; C082018:sea protein, Gallus gallus; D011505:Protein-Tyrosine Kinases