Polar angle as a determinant of amphipathic alpha-helix-lipid interactions: a model peptide study.
Various physicochemical properties play important roles in the membrane activities of amphipathic antimicrobial peptides. To examine the effects of the polar angle, two model peptides, thetap100 and thetap180, with polar angles of 100 degrees and 180 degrees, respectively, were designed, and their interactions with membranes were investigated in detail. These peptides have almost identical physicochemical properties except for polar angle. Like naturally occurring peptides, these peptides selectively bind to acidic membranes, assuming amphipathic alpha-helices, and formed peptide-lipid supramolecular complex pores accompanied by lipid flip-flop and peptide translocation. Despite its somewhat lower membrane affinity, thetap100 exhibited higher membrane permeabilization activity, a greater flip-flop rate, as well as more antimicrobial activity due to a higher pore formation rate compared with thetap180. Consistent with these results, the peptide translocation rate of thetap100 was higher. Furthermore, the number of peptides constituting thetap100 pores was less than that of thetap180, and thetap100 pores involved more lipid molecules, as reflected by its cation selectivity. The polar angle was found to be an important parameter determining peptide-lipid interactions.
Uematsu
N
N
Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto 606-8501, Japan.
Matsuzaki
K
K
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology; D055592:Biophysical Phenomena; D001703:Biophysics; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002942:Circular Dichroism; D015195:Drug Design; D004926:Escherichia coli / Q000187:drug effects; D066298:In Vitro Techniques; D008563:Membrane Lipids / Q000737:chemistry*; D008567:Membranes, Artificial; D008956:Models, Chemical; D008969:Molecular Sequence Data; D010455:Peptides; D010539:Permeability; D011485:Protein Binding; D017433:Protein Structure, Secondary
D000900:Anti-Bacterial Agents; D008563:Membrane Lipids; D008567:Membranes, Artificial; D010455:Peptides