Hemoglobin-sulfhydryls from tortoise (Geochelone carbonaria) can reduce oxidative damage induced by organic hydroperoxide in erythrocyte membrane.
Sulfhydryl groups are important to avoid oxidative damage to the cell. In RBC, tert-butyl hydroperoxide (tert-BOOH) and hydrogen peroxide (H2O2) are capable of oxidizing heme and promoting lipid peroxidation. H2O2 caused greater oxidation of heme than tert-BOOH, although the oxidation of sulfhydryl groups was similar. Geochelone carbonaria Hb, a rich sulfhydryl protein, inhibited the TBA-reactive substances formation of human erythrocytes exposed to tert-BOOH by about 30%; this decrease was smaller with Geochelone denticulata Hb. Sulfhydryl reagents diminished the number of reactive sulfhydryl groups in the G. carbonaria Hb resulting in a decrease of its antioxidant power, suggesting the involvement of sulfhydryls of Hb in the protection against lipid peroxidation.
Torsoni
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Instituto de Ciências da Saúde, Universidade Braz Cubas, Mogi das Cruzes, SP, Brazil. torsoni@yahoo.com
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D000818:Animals; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism*; D006454:Hemoglobins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects*; D010084:Oxidation-Reduction; D013438:Sulfhydryl Compounds / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014426:Turtles / Q000097:blood*; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
D006454:Hemoglobins; D013438:Sulfhydryl Compounds; D017392:Thiobarbituric Acid Reactive Substances; D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide