The 4 A X-ray structure of a tubulin:stathmin-like domain complex.
Phosphoproteins of the stathmin family interact with the alphabeta tubulin heterodimer (tubulin) and hence interfere with microtubule dynamics. The structure of the complex of GDP-tubulin with the stathmin-like domain of the neural protein RB3 reveals a head-to-tail assembly of two tubulins with a 91-residue RB3 alpha helix in which each copy of an internal duplicated sequence interacts with a different tubulin. As a result of the relative orientations adopted by tubulins and by their alpha and beta subunits, the tubulin:RB3 complex forms a curved structure. The RB3 helix thus most likely prevents incorporation of tubulin into microtubules by holding it in an assembly with a curvature very similar to that of the depolymerization products of microtubules.
Gigant
B
B
Laboratoire d'Enzymologie et Biochimie Structurales CNRS UPR 9063, Gif sur Yvette, France.
Curmi
P A
PA
Martin-Barbey
C
C
Charbaut
E
E
Lachkar
S
S
Lebeau
L
L
Siavoshian
S
S
Sobel
A
A
Knossow
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D001923:Brain Chemistry; D002417:Cattle; D018360:Crystallography, X-Ray; D019281:Dimerization; D008868:Microtubule Proteins; D008870:Microtubules / Q000737:chemistry; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D050777:Stathmin; D014404:Tubulin / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism
D008868:Microtubule Proteins; D010750:Phosphoproteins; D050777:Stathmin; D014404:Tubulin