Sensitivities of eupnea and gasping to alterations in temperature of in vivo and perfused rat preparations.
Severe hypoxia or ischemia causes an alteration from eupnea to gasping. At body temperatures approximating 37 degrees C in vivo, the frequency of gasping is much less than that of eupnea. However in a perfused juvenile rat preparation, which is maintained at 30-31 degrees C, the frequency of eupnea and gasping is the same. We hypothesized that brainstem mechanisms responsible for the neurogenesis of eupnea and gasping might have different sensitivities to alterations in temperature. In both decerebrate adult rats in vivo and in a perfused juvenile rat preparation, eupnea and gasping had different frequencies at rectal or perfusate temperatures in excess of 34 degrees C, whereas, at lower temperatures, eupnea and gasping had identical frequencies in both preparations. These findings support the conclusion that different brainstem mechanisms underlie the neurogenesis of eupnea and gasping. In addition, these findings have implications for interpretation of results from in vitro mammalian preparations, which are examined at temperatures at which the frequency of eupnea and gasping are indistinguishable.
St Jacques
R
R
Department of Physiology, Dartmouth Medical School, Dartmouth-Hitchcock Medical Center, Borwell Building, Lebanon, NH 03756, USA.
St-John
W M
WM
D000818:Animals; D001933:Brain Stem / Q000502:physiology; D004558:Electric Stimulation; D004566:Electrodes; D005260:Female; D007511:Ischemia / Q000503:physiopathology; D008297:Male; D023421:Models, Animal; D010477:Perfusion; D010791:Phrenic Nerve / Q000502:physiology; D012123:Pulmonary Ventilation / Q000502:physiology*; D051381:Rats; D012119:Respiration; D013696:Temperature