Differential effects of fluoxetine on murine B-cell proliferation depending on the biochemical pathways triggered by distinct mitogens.
The effect of fluoxetine on mitogen-induced B-cell proliferation was studied. In particular, we analyzed the influence of fluoxetine on the signal transduction pathways triggered after stimulation with lipopolysaccharide (LPS) and anti-immunoglobulin M antibodies (anti-IgM). We showed that fluoxetine had a dual effect on anti-IgM-stimulated B-cell proliferation: at optimal anti-IgM concentration, fluoxetine inhibited proliferation, whereas at suboptimal anti-IgM concentration, the drug enhanced proliferation. Fluoxetine exerted only an inhibitory effect on LPS-induced B-cell proliferation. Calcium influx seemed to be involved in these effects.
Genaro
A M
AM
Centro de Estudios Farmacológicos y Botánicos (CEFYBO-CONICET), Buenos Aires, Argentina. vedgar@cefybo.edu.ar
Edgar
V A
VA
Sterin-Borda
L
L
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000494:pharmacology*; D018687:Antidepressive Agents, Second-Generation / Q000494:pharmacology; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects*; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D004347:Drug Interactions; D005260:Female; D005473:Fluoxetine / Q000494:pharmacology*; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C
D000888:Antibodies, Anti-Idiotypic; D018687:Antidepressive Agents, Second-Generation; D008070:Lipopolysaccharides; C044203:anti-IgM; D005473:Fluoxetine; D002118:Calcium