Myosin motor domain lever arm rotation is coupled to ATP hydrolysis.
We have investigated coupling of lever arm rotation to the ATP binding and hydrolysis steps for the myosin motor domain. In several current hypotheses of the mechanism of force production by muscle, the primary mechanical feature is the rotation of a lever arm that is a subdomain of the myosin motor domain. In these models, the lever arm rotates while the myosin motor domain is free, and then reverses the rotation to produce force while it is bound to actin. These mechanical steps are coupled to steps in the ATP hydrolysis cycle. Our hypothesis is that ATP hydrolysis induces lever arm rotation to produce a more compact motor domain that has stored mechanical energy. Our approach is to use transient electric birefringence techniques to measure changes in hydrodynamic size that result from lever arm rotation when various ligands are bound to isolated skeletal muscle myosin motor domain in solution. Results for ATP and CTP, which do support force production by muscle fibers, are compared to those of ATPgammaS and GTP, which do not. Measurements are also made of conformational changes when the motor domain is bound to NDP's and PP(i) in the absence and presence of the phosphate analogue orthovanadate, to determine the roles the nucleoside moieties of the nucleotides have on lever arm rotation. The results indicate that for the substrates investigated, rotation does not occur upon substrate binding, but is coupled to the NTP hydrolysis step. The data are consistent with a model in which only substrates that produce a motor domain-NDP-P(i) complex as the steady-state intermediate make the motor domain more compact, and only those substrates support force production.
Highsmith
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S
Department of Biochemistry, University of the Pacific, School of Dentistry, San Francisco, California 94115-2399, USA. shighsmith@uop.edu
Polosukhina
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K
Eden
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D
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D001718:Birefringence; D004563:Electrochemistry; D006868:Hydrolysis; D046911:Macromolecular Substances; D020409:Molecular Motor Proteins / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology*; D018482:Muscle, Skeletal / Q000737:chemistry / Q000502:physiology; D015879:Myosin Subfragments / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology*; D017434:Protein Structure, Tertiary; D011817:Rabbits; D012399:Rotation; D013050:Spectrometry, Fluorescence
D046911:Macromolecular Substances; D020409:Molecular Motor Proteins; D015879:Myosin Subfragments; D000255:Adenosine Triphosphate