Characterisation of sugar residues in glycoconjugates of pig mandibular gland by traditional and lectin histochemistry.
Sugar residues are important components of salivary gland secretion. Traditional histochemical methods and lectin histochemistry were used to characterise glycoconjugates present in the mandibular gland of normal adult pigs. Acinar cells contained abundant quantities of glycoconjugates with the terminal trisaccharide sialic acid - (alpha 2-->3, 6) galactosyl (beta 1-->3) N -acetylgalactosamine. Mandibular acinar cells also contained alpha and beta N -acetylgalactosamine and N -acetylglucosamine residues, whereas the demilunar cells contained glycoconjugates with fucose, mannose and N -acetylglucosamine residues. In the duct system a range of sugar residues were localised throughout the cell cytoplasm or limited to the apical surface. These results provide new knowledge concerning the structure of salivary glycoconjugates in normal adult pig and a basis for future pathological studies.
Pedini
V
V
Dipartimento di Scienze Biopatologiche Veterinarie, Sezione di Anatomia, Via San Costanzo 4, 06126 Perugia, Italy.
Scocco
P
P
Dall'Aglio
C
C
Ceccarelli
P
P
Gargiulo
A M
AM
D000117:Acetylglucosamine / Q000032:analysis; D000818:Animals; D002241:Carbohydrates / Q000737:chemistry*; D005260:Female; D007124:Immunoenzyme Techniques / Q000662:veterinary; D037102:Lectins / Q000737:chemistry*; D008297:Male; D009439:Neuraminidase / Q000378:metabolism; D013363:Submandibular Gland / Q000737:chemistry* / Q000166:cytology; D013552:Swine
D002241:Carbohydrates; D037102:Lectins; D009439:Neuraminidase; D000117:Acetylglucosamine