title: Renibacterium salmoninarum isolates from different sources possess two highly conserved copies of the rRNA operon .
abstract: The nucleotide sequences of the rRNA genes and the 5' flanking region were determined for R. salmoninarum ATCC 33209T from overlapping products generated by PCR amplification from the genomic DNA. Comparison of the sequences with rRNA genes from a variety of bacteria demonstrated the close relatedness between R. salmoninarum and the high G+C group of the actinobacteria, in particular, Arthrobacter species. A regulatory element within the 5' leader of the rRNA operon was identical to an element, CL2, described for mycobacteria. PCR, DNA sequence analysis, and DNA hybridisation were performed to examine variation between isolates from diverse sources which represented the four 16S-23S rRNA intergenic spacer sequevars previously described for R. salmoninarum. Two 23S-5S rRNA intergenic spacer sequevars of identical length were found. DNA hybridisation using probes complementary to 23S rDNA and 16S rDNA identified two rRNA operons which were identical or nearly identical amongst 40 isolates sourced from a variety of countries.
authors.affiliation: Department of Biological Sciences, University of Plymouth, Devon, UK. tgrayson@plymouth.ac.uk
authors.forename: T H
authors.initials: TH
authors.lastname: Grayson
authors.forename: S M
authors.initials: SM
authors.lastname: Alexander
authors.forename: L F
authors.initials: LF
authors.lastname: Cooper
authors.forename: M L
authors.initials: ML
authors.lastname: Gilpin
mesh_terms: D000192:Actinomycetales / Q000235:genetics; D001483:Base Sequence; D017124:Conserved Sequence; D004275:DNA, Ribosomal / Q000235:genetics*; D018628:Gene Dosage; D014644:Genetic Variation; D016989:Gram-Positive Asporogenous Rods, Regular / Q000235:genetics*; D008969:Molecular Sequence Data; D009876:Operon; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012335:RNA, Ribosomal / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA
chemical_list: D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D012341:RNA, Ribosomal, 5S