Molecular cloning and expression of rat betacellulin cDNA.
The cDNA encoding an entire open reading frame of rat betacellulin has been cloned from rat kidney. Expression of this cDNA in COS7 cells showed a significant amount of mitogenic activity in the culture media. Western blotting of the cell lysates suggested that the membrane-anchored precursor was cleaved to release its ectodomain very efficiently.
Tada
H
H
Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, Tsushima-naka, Okayama, Japan. tadahrk@biotech.okayama-u.ac.jp
Seno
M
M
Yamada
H
H
Sasada
R
R
Igarashi
K
K
D016475:3T3 Cells / Q000187:drug effects; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D066259:Betacellulin; D019556:COS Cells; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis; D004815:Epidermal Growth Factor / Q000737:chemistry; D015870:Gene Expression; D006133:Growth Substances / Q000235:genetics* / Q000494:pharmacology; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D007668:Kidney / Q000502:physiology; D051379:Mice; D008934:Mitogens / Q000494:pharmacology; D008969:Molecular Sequence Data; D051381:Rats; D017386:Sequence Homology, Amino Acid; D014407:Tumor Cells, Cultured
C587851:BTC protein, human; D066259:Betacellulin; C587852:Btc protein, mouse; C587853:Btc protein, rat; D018076:DNA, Complementary; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D008934:Mitogens; D004815:Epidermal Growth Factor