title: Presentation of chemokine SDF-1 alpha by fibronectin mediates directed migration of T cells.
abstract: The role of chemokine-matrix interactions in integrin-dependent T-cell migration was examined to address the critical question of how chemokines provide directional information. The chemokine SDF-1 alpha binds fibronectin (Fn) with a low nanomolar K(d) (equilibrium dissociation constant). SDF-1 alpha presented by Fn induced directed migration. Spatial concentration gradients of chemokine were not required to maintain directed migration. Fn-presented chemokine induced the polarization of cells, including the redistribution of the SDF-1 alpha receptor, to the basal surface and leading edge of the cell. A new model for directed migration is proposed in which the co-presentation of an adhesive matrix and chemokine provides the necessary positional information independent of a soluble spatial gradient. (Blood. 2000;96:2682-2690)
authors.affiliation: Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA, USA.
authors.forename: A J
authors.initials: AJ
authors.lastname: Pelletier
authors.forename: L J
authors.initials: LJ
authors.lastname: van der Laan
authors.forename: P
authors.initials: P
authors.lastname: Hildbrand
authors.forename: M A
authors.initials: MA
authors.lastname: Siani
authors.forename: D A
authors.initials: DA
authors.lastname: Thompson
authors.forename: P E
authors.initials: PE
authors.lastname: Dawson
authors.forename: B E
authors.initials: BE
authors.lastname: Torbett
authors.forename: D R
authors.initials: DR
authors.lastname: Salomon
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D016764:Cell Polarity; D054377:Chemokine CXCL12; D019743:Chemokines, CXC / Q000138:chemical synthesis / Q000494:pharmacology / Q000502:physiology*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel; D005353:Fibronectins / Q000502:physiology*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D019169:Jurkat Cells / Q000166:cytology / Q000187:drug effects; D018613:Microscopy, Confocal; D009994:Osmolar Concentration; D011485:Protein Binding; D019718:Receptors, CXCR4 / Q000502:physiology; D012995:Solubility; D020349:Surface Plasmon Resonance; D013601:T-Lymphocytes / Q000166:cytology* / Q000187:drug effects
chemical_list: C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D019743:Chemokines, CXC; D005353:Fibronectins; D019718:Receptors, CXCR4