title: 17q23 amplifications in breast cancer involve the PAT1, RAD51C, PS6K, and SIGma1B genes.
abstract: Amplification of the 17q23 region occurs frequently in breast tumors. To characterize the structure of 17q23 amplicons and to identify oncogene targets associated with this alteration, we performed a copy number analysis of 87 17q23 localized expressed sequence tags in seven breast cancer cell lines. Three major regions of amplification were detected in the MCF7 and BT474 cell lines. Amplification of at least one of four known genes (PAT1, PS6K, RAD51C, and SIGMA1B) was detected in the cell lines and in 28% of 94 breast tumors. In most cases, these four genes were overexpressed when amplified, but there was a particularly good association between amplification of the SIGMA1B gene and elevated expression in tumors, which suggested a possible role for this gene in tumor progression. Our data show that this region contains at least four independent targets of amplification, which suggests that there is considerable variability in the structure of the 17q23 amplicon.
authors.affiliation: Department of Laboratory Medicine and Pathology, Mayo Clinic and Foundation, Rochester, Minnesota 55905, USA.
authors.forename: G J
authors.initials: GJ
authors.lastname: Wu
authors.forename: C S
authors.initials: CS
authors.lastname: Sinclair
authors.forename: J
authors.initials: J
authors.lastname: Paape
authors.forename: J N
authors.initials: JN
authors.lastname: Ingle
authors.forename: P C
authors.initials: PC
authors.lastname: Roche
authors.forename: C D
authors.initials: CD
authors.lastname: James
authors.forename: F J
authors.initials: FJ
authors.lastname: Couch
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism; D018270:Carcinoma, Ductal, Breast / Q000235:genetics* / Q000378:metabolism; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D005784:Gene Amplification; D015870:Gene Expression; D006801:Humans; D016601:RNA-Binding Proteins; D051135:Rad51 Recombinase; D019893:Ribosomal Protein S6 Kinases / Q000096:biosynthesis / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D018808:Transcription Factor AP-1 / Q000096:biosynthesis / Q000235:genetics; D014407:Tumor Cells, Cultured
chemical_list: D004268:DNA-Binding Proteins; C103266:PAT1 protein, S cerevisiae; D016601:RNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; D018808:Transcription Factor AP-1; D019893:Ribosomal Protein S6 Kinases; C495153:RAD51 protein, human; D051135:Rad51 Recombinase