title: Carotenoid oxidative degradation products inhibit Na+-K+-ATPase.
abstract: This study investigates the biological significance of carotenoid oxidation products using inhibition of Na+-K+-ATPase activity as an index. Beta-carotene was completely oxidized by hypochlorous acid and the oxidation products were analyzed by capillary gas-liquid chromatography and high performance liquid chromatography. The Na+-K+-ATPase activity was assayed in the presence of these oxidized carotenoids and was rapidly and potently inhibited. This was demonstrated for a mixture of beta-carotene oxidative breakdown products, beta-Apo-10'-carotenal and retinal. Most of the beta-carotene oxidation products were identified as aldehydic. The concentration of the oxidized carotenoid mixture that inhibited Na+-K+-ATPase activity by 50% (IC50) was equivalent to 10 microM non-degraded beta-carotene, whereas the IC50 for 4-hydroxy-2-nonenal, a major lipid peroxidation product, was 120 microM. Carotenoid oxidation products are more potent inhibitors of Na+-K+-ATPase than 4-hydroxy-2-nonenal. Enzyme activity was only partially restored with hydroxylamine and/or beta-mercaptoethanol. Thus, in vitro binding of carotenoid oxidation products results in strong enzyme inhibition. These data indicate the potential toxicity of oxidative carotenoid metabolites and their activity on key enzyme regulators and signal modulators.
authors.affiliation: Department of Ophthalmology and Visual Sciences, University of Texas Medical Branch at Galveston, 77555-1067, USA.
authors.forename: W G
authors.initials: WG
authors.lastname: Siems
authors.forename: O
authors.initials: O
authors.lastname: Sommerburg
authors.forename: J S
authors.initials: JS
authors.lastname: Hurst
authors.forename: F J
authors.initials: FJ
authors.lastname: van Kuijk
mesh_terms: D000447:Aldehydes / Q000737:chemistry / Q000494:pharmacology; D002338:Carotenoids / Q000737:chemistry* / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006997:Hypochlorous Acid / Q000737:chemistry; D010084:Oxidation-Reduction; D012172:Retinaldehyde / Q000737:chemistry / Q000494:pharmacology; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*; D019207:beta Carotene / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000447:Aldehydes; D004791:Enzyme Inhibitors; D019207:beta Carotene; D002338:Carotenoids; D006997:Hypochlorous Acid; D000254:Sodium-Potassium-Exchanging ATPase; C027576:4-hydroxy-2-nonenal; D012172:Retinaldehyde