Stability of CSF metabolites measured by proton NMR.
Analysis of cerebrospinal fluid (CSF) metabolites can provide data regarding CNS involvement in neurologic and psychiatric illness. However, there is lack of research into the effect of processing and storage of CSF specimens on the levels of metabolites analyzed. CSF specimens from 10 depressed patients were analyzed by proton NMR before and after 72 hours exposure to room temperature. No effect of exposure was found on myoinositol, glucose, acetate, and alanine CSF levels and there was a substantial decrease of citrate (>50%) and increase in lactate, glutamine, creatine, and creatinine levels.
Levine
J
J
Department of Psychiatry, School of Medicine, University of Pittsburgh, Pennsylvania 15213, USA. levinej@msx.upmc.edu
Panchalingam
K
K
McClure RJ2
Gershon
S
S
Pettegrew
J W
JW
D000596:Amino Acids / Q000134:cerebrospinal fluid; D003866:Depressive Disorder / Q000134:cerebrospinal fluid*; D005947:Glucose / Q000134:cerebrospinal fluid; D006801:Humans; D007294:Inositol / Q000134:cerebrospinal fluid; D019344:Lactic Acid / Q000134:cerebrospinal fluid; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D011522:Protons; D013048:Specimen Handling; D013696:Temperature
D000596:Amino Acids; D011522:Protons; D019344:Lactic Acid; D007294:Inositol; D005947:Glucose