Tryptophan 67 in the human VPAC(1) receptor: crucial role for VIP binding.
The human receptor subtype for VIP and PACAP, referred to as VPAC(1) receptor, has a large N-terminal extracellular domain which is critical for VIP binding. We further investigated this domain by mutating 12 amino acid residues which could participate in the formation of a tight bend (W67) or a coiled coil motif. They were changed to alanine (A) and the cDNAs were transiently transfected into Cos cells. All mutants but W67A exhibited K(d) values similar to that of the wild-type receptor. For the W67A mutant, no specific (125)I-VIP binding could be observed. Mutants at the W67 site were further characterized after stable transfection of epitope-tagged VPAC(1) receptor-GFP fusion proteins into CHO cells. W67A, W67E, W67H, and W67K mutants neither bound VIP nor mediated adenylyl cyclase activation by VIP. The W67F mutant mediated stimulation of adenylyl cyclase only at high VIP concentrations. Microscopic analysis and antibody binding experiments showed that all mutants were similarly expressed at the cell surface of CHO cells. Therefore tryptophan 67 in the human VPAC(1) receptor plays a crucial role in VIP binding due, in part, to its aromatic moiety.
Nicole
P
P
Unité de Neuroendocrinologie et Biologie Cellulaire Digestives, Institut National de la Santé et de la Recherche Médicale, Paris, 75018, France.
Maoret
J J
JJ
Couvineau
A
A
Momany
F A
FA
Laburthe
M
M
D000818:Animals; D001216:Asparagine / Q000378:metabolism; D001224:Aspartic Acid / Q000378:metabolism; D016466:CHO Cells; D019556:COS Cells; D006224:Cricetinae; D000242:Cyclic AMP / Q000378:metabolism; D006801:Humans; D016297:Mutagenesis, Site-Directed; D011487:Protein Conformation; D018005:Receptors, Vasoactive Intestinal Peptide / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051238:Receptors, Vasoactive Intestinal Polypeptide, Type I; D014162:Transfection; D014364:Tryptophan / Q000235:genetics / Q000378:metabolism*; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*
D018005:Receptors, Vasoactive Intestinal Peptide; D051238:Receptors, Vasoactive Intestinal Polypeptide, Type I; D001224:Aspartic Acid; D014660:Vasoactive Intestinal Peptide; D001216:Asparagine; D014364:Tryptophan; D000242:Cyclic AMP