title: Characterization of human SCO1 and COX17 genes in mitochondrial cytochrome-c-oxidase deficiency.
abstract: At least three proteins, COX17p, SCO1p, and its homologue SCO2p are thought to be involved in mitochondrial copper transport to cytochrome-c-oxidase (COX), the terminal enzyme of the respiratory chain. Recently, we and others have shown that mutations in SCO2 are associated with a lethal infantile hypertrophic cardiomyopathy (HCMP) with COX-deficiency. The majority of patients with a similar phenotype were, however, negative for SCO2 mutations, suggesting the other genes as candidates for this disorder. Here we report on the genomic organization of SCO1 and COX17 on human chromosomes 17 and 3 respectively, and the complete sequence analysis of COX17 and SCO1 in 30 patients with COX deficiency. Using a panel of human:mouse-monochromosomal hybrids, the expression of COX17 was specifically restricted to chromosome 3, indicating that the previously reported sequence on chromosome 13 represents a pseudogene. DNA sequence analysis of SCO1 and COX17 in nine patients with severe COX deficiency and fatal HCMP, and in 21 patients with other COX deficiency disorders, did not reveal any pathogenic mutations or polymorphisms. We conclude that neither SCO1 nor COX17 are common causes of COX deficiency disorders.
authors.affiliation: Metabolic Disease Centre Munich, Institute of Clinical Chemistry, Munich, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Horvath
authors.forename: H
authors.initials: H
authors.lastname: Lochmüller
authors.forename: R
authors.initials: R
authors.lastname: Stucka
authors.forename: J
authors.initials: J
authors.lastname: Yao
authors.forename: E A
authors.initials: EA
authors.lastname: Shoubridge
authors.forename: S H
authors.initials: SH
authors.lastname: Kim
authors.forename: K D
authors.initials: KD
authors.lastname: Gerbitz
authors.forename: M
authors.initials: M
authors.lastname: Jaksch
mesh_terms: D006332:Cardiomegaly / Q000235:genetics; D002352:Carrier Proteins; D027682:Cation Transport Proteins; D002874:Chromosome Mapping; D002886:Chromosomes, Human, Pair 17; D002893:Chromosomes, Human, Pair 3; D000079922:Copper Transport Proteins; D030401:Cytochrome-c Oxidase Deficiency; D003576:Electron Transport Complex IV / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D024101:Mitochondrial Proteins; D018832:Molecular Chaperones; D009154:Mutation; D010641:Phenotype; D011506:Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: C491006:COX17 protein, human; D002352:Carrier Proteins; D027682:Cation Transport Proteins; D000079922:Copper Transport Proteins; C491005:Cox17 protein, mouse; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D018832:Molecular Chaperones; D011506:Proteins; C489345:SCO1 protein, human; C400285:SCO2 protein, human; D003576:Electron Transport Complex IV