title: Interaction of cyanine dyes with nucleic acids. Part 19: new method for the covalent labeling of oligonucleotides with pyrylium cyanine dyes.
abstract: New chemistry for the fluorescent labeling of oligonucleotides with cyanine dyes is proposed. It is based on the use of pyrylium salts as amine-specific reagents. Monomethyne pyrylium cyanine dye 1 was covalently linked to 5'-aminoalkyl modified oligonucleotide, with simultaneous conversion of the non-fluorescent dye 1 into fluorescent pyridinium cyanine structure 2.
authors.affiliation: Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv. otava@carrier.kiev.ua
authors.forename: S M
authors.initials: SM
authors.lastname: Yarmoluk
authors.forename: A M
authors.initials: AM
authors.lastname: Kostenko
authors.forename: I Y
authors.initials: IY
authors.lastname: Dubey
mesh_terms: D000588:Amines / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D015394:Molecular Structure; D009841:Oligonucleotides / Q000737:chemistry* / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods*; D013844:Thiazoles / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000588:Amines; C419203:Cyan 39; D005456:Fluorescent Dyes; D009841:Oligonucleotides; D013844:Thiazoles