Gibbestatin B inhibits the GA-induced expression of alpha-amylase expression in cereal seeds.
The expression of alpha-amylase in aleurone layers of barley is known to be induced by gibberellin A3 (GA). In the present study, gibbestatin B (GNB) was isolated from Streptomyces sp. C-39 as an inhibitor of the GA-induced expression of alpha-amylase in barley and rice, with IC50 values of 125 and 70 microM, respectively. GNB suppressed accumulation of GA-induced barley high-pI type B and rice RAmylA alpha-amylase transcripts. However, GNB showed no inhibitory activity on GUS expression in transgenic tobacco harboring the auxin-inducible par B promoter:: GUS fusion gene. The transcription of an abscisic acid (ABA)-inducible gene, HVA1, was unaffected by GNB. In addition, GNB prevented aleurone cells from cell death induced by GA. In tobacco and Arabidopsis plants, GNB suppressed the germination and retarded the growth of seedlings without toxicity. The growth of gai, spy and abi mutants was also retarded by GNB. Normal plants treated with GA-biosynthesis inhibitors and GA-defective and GA-signaling mutants normally have dwarf dark green leaves. However, dwarfed healthy green leaves were observed in normal plants treated with GNB. GA-induced stem elongation of plants was also detected in the presence of GNB. These analyses indicate that GNB inhibits the GA-induced expression of alpha-amylase by regulating one of the steps involved in ABA signaling, but not by acting as a weak ABA analog.
Hayashi
K
K
Department of Biochemistry, Okayama University of Science, Japan. hayashi@dbc.ous.ac.jp
Inoguchi
M
M
Kondo
H
H
Nozaki
H
H
D000040:Abscisic Acid / Q000502:physiology; D016923:Cell Death / Q000502:physiology; D004852:Epoxy Compounds / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D005875:Gibberellins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D001467:Hordeum / Q000201:enzymology*; D007210:Indoleacetic Acids / Q000502:physiology; D012275:Oryza / Q000201:enzymology*; D010648:Phenylacetates / Q000494:pharmacology*; D000516:alpha-Amylases / Q000235:genetics / Q000378:metabolism*
D004852:Epoxy Compounds; D005875:Gibberellins; D007210:Indoleacetic Acids; D010648:Phenylacetates; C402699:gibbestatin B; D000040:Abscisic Acid; C007842:gibberellic acid; D000516:alpha-Amylases