title: Expression of the zinc finger gene fez-like in zebrafish forebrain.
abstract: Anterior-posterior (A-P) patterning in the neuroectoderm is established during gastrulation in zebrafish and amphibians. We isolated a novel zinc-finger gene fez-like (fezl) from zebrafish, which displays sequence similarities to Xenopus Fez. The fezl transcripts were detected in the anterior edge of neuroectoderm, the prospective dorsal forebrain, from the late gastrula (80% epiboly stage) to the mid-segmentation period. fezl was also expressed in the ventral forebrain overlying the prechordal plate at these stages. The expression of fezl was enhanced in embryos expressing the Wnt inhibitor Dkk1 and reduced in embryos expressing Wnt8b. The expression in the ventral forebrain was eliminated in the one-eyed pinhead mutant and the antivin RNA-injected embryos, which lack the prechordal plate. Radiation hybrid mapping revealed that the fezl gene is localized to linkage group 11.
authors.affiliation: Division of Molecular Oncology (C7), Biomedical Research Center, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, Suita, 565-0871, Osaka, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Hashimoto
authors.forename: T
authors.initials: T
authors.lastname: Yabe
authors.forename: T
authors.initials: T
authors.lastname: Hirata
authors.forename: T
authors.initials: T
authors.lastname: Shimizu
authors.forename: Y
authors.initials: Y
authors.lastname: Bae
authors.forename: Y
authors.initials: Y
authors.lastname: Yamanaka
authors.forename: T
authors.initials: T
authors.lastname: Hirano
authors.forename: M
authors.initials: M
authors.lastname: Hibi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000235:genetics; D015870:Gene Expression; D036341:Intercellular Signaling Peptides and Proteins; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D016548:Prosencephalon / Q000196:embryology / Q000378:metabolism*; D011506:Proteins / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D029867:Xenopus Proteins; D015027:Zebrafish; D029961:Zebrafish Proteins; D016335:Zinc Fingers
chemical_list: D002352:Carrier Proteins; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C501127:Dkk1 protein, mouse; C485861:FEZF1 protein, Xenopus; D036341:Intercellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; D011506:Proteins; D029867:Xenopus Proteins; D029961:Zebrafish Proteins; C412364:Zfp312 protein, mouse; C501128:dkk1b protein, zebrafish; C418489:fezf2 protein, zebrafish