Inhibition of lens epithelial cell proliferation by substituted PMMA intraocular lenses.
BACKGROUND
Migration and proliferation of lens epithelial cells (LECs) are based on interactions between cellular integrins and extracellular matrix proteins (ECMs). We were able to design polymers substituted with COO- and SO3- groups that counteract LEC proliferation through modification of the integrin/ECM interaction. The objective of this study was to compare the cell proliferation-inhibiting properties of differently substituted copolymers with those of a homopolymer.


METHODS
Polymers were synthesized by radical polymerization and characterized by nuclear resonance spectroscopy. Second-passage rabbit LECs were cultured either on PMMA homopolymers (control) or on substituted PMMA copolymers. Cells were counted on days 2, 7, and 17. Cell vitality was evaluated by trypan blue staining. Experiments were run in quadruplicate. Statistical analysis was performed using the Wilcoxon test and the Mann-Whitney U-test.


RESULTS
Compared with control, cells cultured on copolymers grew to significantly lower cell numbers. The inhibitory effect became evident at day 2 after seeding and persisted throughout the experiment. Different ratios of COO-/SO3- substitutions yielded different grades of inhibition. No toxic effect was seen on rabbit LECs.


CONCLUSIONS
The tested copolymers inhibit cell proliferation and are nontoxic. Further evaluation could lead to the development of new intraocular lens materials that prevent secondary cataract.
Latz
C
C
Department of Ophthalmology, Humboldt University, Berlin, Germany.
Migonney
V
V
Pavon-Djavid
G
G
Rieck
P
P
Hartmann
C
C
Renard
G
G
Legeais
J M
JM
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D002448:Cell Adhesion; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects*; D005260:Female; D007908:Lens, Crystalline / Q000166:cytology*; D007910:Lenses, Intraocular; D009682:Magnetic Resonance Spectroscopy; D019904:Polymethyl Methacrylate / Q000737:chemistry / Q000494:pharmacology*; D011817:Rabbits
D001672:Biocompatible Materials; D019904:Polymethyl Methacrylate