title: Identification of novel transcription factor-like gene from human intestinal cells.
abstract: Intestinal crypt epithelial T84 cells form luminal structures and differentiate to intestinal enterocyte-like cells in response to IMR-90 fibroblast-secreted transforming growth factor-beta when grown within three-dimensional collagen gel. In search of TGF-beta regulated genes involved in this differentiation process, we isolated a TGF-beta downregulated cDNA, human homologue of rat apoptosis antagonising transcription factor that codes for a 560-amino-acid protein. Human AATF-mRNA was expressed at high levels in human brain, heart, thymus, kidney, and placenta while in skeletal muscle and colon the expression was lower. The gene was mapped to chromosome 17q11.2-q12.
authors.affiliation: Institute of Medical Technology, Tampere, FIN-33014, Finland.
authors.forename: K
authors.initials: K
authors.lastname: Lindfors
authors.forename: T
authors.initials: T
authors.lastname: Halttunen
authors.forename: P
authors.initials: P
authors.lastname: Huotari
authors.forename: N
authors.initials: N
authors.lastname: Nupponen
authors.forename: M
authors.initials: M
authors.lastname: Vihinen
authors.forename: T
authors.initials: T
authors.lastname: Visakorpi
authors.forename: M
authors.initials: M
authors.lastname: Mäki
authors.forename: H
authors.initials: H
authors.lastname: Kainulainen
mesh_terms: D000595:Amino Acid Sequence; D051017:Apoptosis Regulatory Proteins; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D002874:Chromosome Mapping; D002886:Chromosomes, Human, Pair 17; D005786:Gene Expression Regulation; D006801:Humans; D007422:Intestines / Q000166:cytology / Q000502:physiology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012097:Repressor Proteins; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000502:physiology
chemical_list: C402637:AATF protein, human; D051017:Apoptosis Regulatory Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; D016212:Transforming Growth Factor beta