title: Cytologic diagnosis of leishmaniasis in HIV infection. A report of eight cases.
abstract: BACKGROUND
  Leishmania organisms are among the intracellular microorganisms with a tendency to develop in patients with the acquired immunodeficiency syndrome (AIDS). With increasing travel to endemic areas by patients with human immunodeficiency virus (HIV) infection, it is becoming a more-frequent diagnosis in nonendemic areas.
  
  
  METHODS
  Ten cytologic specimens from eight patients with leishmaniasis and AIDS were reviewed. Eight samples were obtained from lymph nodes through fine needle aspiration (FNA). Another sample was obtained after scraping a tongue ulcer. The last one was an ascitic fluid specimen. Smears showed numerous parasitized histiocytes with abundant intracellular Leishmania organisms (amastigotes). Extracellular microorganisms were also abundant. Diff-Quik-stained smears allowed the clear recognition of the characteristic morphologic appearance with a deep-staining area (nuclei) and paranuclear zone (kinetoplast). Intracellular organisms were round, while single, extracellular forms were a more elongated.
  
  
  CONCLUSIONS
  The polymorphous clinical manifestations usually seen in patients suffering from leishmaniasis and AIDS constitute a diagnostic challenge that can be facilitated by cytopathologic examination. Cytology permits easy and rapid identification of Leishmania amastigotes, allowing a specific diagnosis and treatment.
authors.affiliation: Department of Pathology, Hospital Universitario La Paz, Madrid, Spain.
authors.forename: B
authors.initials: B
authors.lastname: Vicandi
authors.forename: J A
authors.initials: JA
authors.lastname: Jiménez-Heffernan
authors.forename: P
authors.initials: P
authors.lastname: López-Ferrer
authors.forename: L
authors.initials: L
authors.lastname: Ortega
authors.forename: J M
authors.initials: JM
authors.lastname: Viguer
mesh_terms: D000328:Adult; D000818:Animals; D001707:Biopsy, Needle; D015658:HIV Infections / Q000150:complications*; D006801:Humans; D007891:Leishmania / Q000302:isolation & purification*; D007896:Leishmaniasis / Q000175:diagnosis* / Q000209:etiology / Q000473:pathology; D008297:Male