Galectin-1 gene expression and methylation state in human T leukemia cell lines.
Galectin-1 has been demonstrated to be a mediator of T-cell apoptosis acting on activated T-cells and, in a selective manner, on different T leukemia cell lines. Here we show that the sensitivity to galectin-1 is associated with repression of the endogenous galectin-1 gene whereas non-sensitive cells express high levels of galectin-1. Repression of galectin-1 gene in sensitive cells is associated with hyper-methylation of the promoter region. Transient treatment of non-expressing cells with the demethylating agent 5-azacytidine led to irreversible demethylation and subsequent reactivation of galectin-1 gene.
Salvatore
P
P
Dipartimento di Scienze Ambientali, 2a Universita degli Studi di Napoli, 81100 Caserta, Italy.
Benvenuto
G
G
Pero
R
R
Lembo
F
F
Bruni
C B
CB
Chiariotti
L
L
D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology; D001374:Azacitidine / Q000494:pharmacology; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000037:antagonists & inhibitors / Q000378:metabolism; D019175:DNA Methylation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D037483:Galectin 1; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects; D006388:Hemagglutinins / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D015458:Leukemia, T-Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D009363:Neoplasm Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
D000964:Antimetabolites, Antineoplastic; D004791:Enzyme Inhibitors; D037483:Galectin 1; D006388:Hemagglutinins; D009363:Neoplasm Proteins; D004248:DNA (Cytosine-5-)-Methyltransferases; D001374:Azacitidine