Microscale diversity of the genus Nitrobacter in soil on the basis of analysis of genes encoding rRNA.
We looked at the diversity of [NO(2)](-) oxidizers at field scale by examining isolates at clump scale and in microsamples of soil (diameter, 50 microm). The genetic distances (as determined by amplified ribosomal DNA restriction analysis performed with Nitrobacter-specific primers) in a small clump of soil were as large as those between reference strains from large geographical areas. Diversity in individual microsamples was shown by serotyping.
Grundmann
G L
GL
Laboratoire d'Ecologie Microbienne, UMR 5557 Université Claude Bernard, 69622 Villeurbanne Cedex, France. grundman@biomserv.univ-lyon1.fr
Normand
P
P
D005075:Biological Evolution; D017931:DNA Primers; D004275:DNA, Ribosomal / Q000235:genetics*; D005798:Genes, Bacterial; D014644:Genetic Variation; D005838:Genotype; D009566:Nitrates / Q000378:metabolism; D009576:Nitrobacter / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D010084:Oxidation-Reduction; D010802:Phylogeny; D015183:Restriction Mapping; D012988:Soil Microbiology
D017931:DNA Primers; D004275:DNA, Ribosomal; D009566:Nitrates