Cloning, expression, and mapping of hWW45, a novel human WW domain-containing gene.
WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. The cloning and characterization of a novel human WW domain-containing gene, hWW45, which encodes a protein of approximately 45 kDa consisting of 2 WW domains and a coiled-coil region is reported here. The murine homologue cDNA, mWW45, displays a different 3'-untranslated region and predicts a protein identity of 93% to hWW45. Northern blot and RT-PCR analysis demonstrated that both mWW45 and hWW45 transcripts are ubiquitously expressed in adult tissues. The mouse embryonic expression is first seen at 7 days post coitum in Northern blot analysis of whole embryos. Chromosomal localization by radiation hybrid mapping revealed that hWW45 is localized at chromosome 14, 10.31cR from the marker D14S269.
Valverde
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Harvard/Forsyth Department of Oral Biology, Forsyth Institute, 140 Fenway, Boston, Massachusetts, 02115, USA. pvalerde@forsyth.org
D020413:3' Untranslated Regions / Q000235:genetics; D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018797:Cell Cycle Proteins; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics*; D003001:Cloning, Molecular; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis / Q000737:chemistry; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry* / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D021101:Radiation Hybrid Mapping; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein
D020413:3' Untranslated Regions; D018797:Cell Cycle Proteins; D010455:Peptides; D011506:Proteins; D012333:RNA, Messenger; C415864:SAV1 protein, human; C485935:Sav1 protein, mouse