Amplification of herpes simplex virus type 1 DNA in human geniculate ganglia from formalin-fixed, nonembedded temporal bones.
Polymerase chain reaction (PCR) has provided new insights in molecular biology. Recently, some studies have been focused on temporal bone pathology, with amplification of DNA from fixed sections of celloidin-embedded bones. The purpose of our study was to elucidate the utility of PCR in detection of minor concentrations of DNA from nonoptimal stored samples. We obtained geniculate ganglia from 30 temporal bones preserved in formalin for a long time, without any process of embedding. By performing a nested PCR assay, we detected herpes simplex virus type 1 DNA in 13 of 30 ganglia (43%). We conclude therefore that study of temporal bones stored under poor conditions by PCR is possible, although there are some limitations when compared with fresh or optimally archived samples.
Carreño
M
M
Department of Otolaryngology, Hospital Central de Asturias, Universidad de Oviedo, Spain.
Oña
M
M
Melón
S
S
Llorente
J L
JL
Díaz
J J
JJ
Suarez
C
C
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D002648:Child; D003470:Culture Media; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005404:Fixatives; D005557:Formaldehyde; D005830:Geniculate Ganglion / Q000821:virology*; D018259:Herpesvirus 1, Human / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013701:Temporal Bone / Q000821:virology*
D003470:Culture Media; D004279:DNA, Viral; D005404:Fixatives; D005557:Formaldehyde