Toxicity assessment of fumonisins using the brine shrimp (Artemia salina) bioassay.
The Fusarium mycotoxins fumonisin B(1) (FB(1)) (1) and B(2) (FB(2)) (2), their hydrolysed analogues HFB(1) (3) and HFB(2) (4) and the recently discovered fumonisin derivatives N-palmitoyl-HFB(1) (5) and N-carboxymethyl-FB(1) (6) were compared for their toxicity in a short term bioassay using brine shrimp (Artemia salina). The brine shrimp were hatched in artificial sea water and exposed to the fumonisins in microwell plates with a mortality endpoint after 48 hours. LC(50) values were calculated after Probit transformation of the resulting data. Of the substances tested, fumonisin B(1) emerged to be the most toxic whereas its N-carboxymethyl analogue was 100-fold less effective. The hydrolysed fumonisins showed a four- to sixfold reduced toxicity compared to FB(1). N-Palmitoyl-HFB(1) had a higher LC(50) value than its precursor HFB(1). The brine shrimp assay proved to be a convenient and rapid system for toxicity assessment of this group of mycotoxins.
Hartl
M
M
Lehrstuhl für Lebensmittelchemie, Universität Würzburg, Am Hubland, 97074, Würzburg, Germany.
Humpf
H U
HU
D000818:Animals; D001156:Artemia; D001681:Biological Assay; D002264:Carboxylic Acids / Q000633:toxicity*; D002274:Carcinogens, Environmental / Q000633:toxicity*; D004791:Enzyme Inhibitors / Q000633:toxicity*; D037341:Fumonisins; D007928:Lethal Dose 50; D009183:Mycotoxins / Q000633:toxicity*
D002264:Carboxylic Acids; D002274:Carcinogens, Environmental; D004791:Enzyme Inhibitors; D037341:Fumonisins; D009183:Mycotoxins; C056934:fumonisin B2; C056933:fumonisin B1