Biocompatibility and performance in vitro of a hemostatic gelatin sponge.
The biocompatibility of a hemostatic gelatin sponge (Cutanplast Standard) was evaluated in vitro. Cytotoxicity was assessed by neutral red uptake and amido black staining tests; genotoxicity was assayed using the Ames test, Sister Chromatides Exchanges (SCE) and chromosomal aberrations. The ability of the hemostatic gelatin sponge to induce platelet adhesion and release reaction was also determined. The undiluted extract of the test material was found to be cytotoxic, but cell viability was not affected by 1:2 and 1:10 diluted extract. The same extract was found to be non-genotoxic using the three assays for genotoxicity. A significant decrease of platelet number, as well as a significant release of platelet factor 4 was found to be caused by the solid material. In conclusion, Cutanplast Standard is neither cytotoxic nor genotoxic, while inducing platelet adhesion and release reaction when challenged with blood.
Cenni
E
E
Dipartimento Putti, Istituti Ortopedici Rizzoli, Bologna, Italy. elisabetta.cenni@ior.it
Ciapetti
G
G
Stea
S
S
Corradini
A
A
Carozzi
F
F
D020341:Absorbable Implants; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology* / Q000633:toxicity; D016466:CHO Cells; D002470:Cell Survival / Q000187:drug effects; D002869:Chromosome Aberrations; D006224:Cricetinae; D005780:Gelatin / Q000494:pharmacology* / Q000633:toxicity; D006489:Hemostatic Techniques; D006801:Humans; D007739:L Cells; D051379:Mice; D009152:Mutagenicity Tests; D010976:Platelet Count; D010978:Platelet Factor 4 / Q000378:metabolism*; D012486:Salmonella typhimurium / Q000187:drug effects; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D013552:Swine
D001672:Biocompatible Materials; D010978:Platelet Factor 4; D005780:Gelatin