title: Tumor necrosis factor-alpha modulates the proliferation of neural progenitors in the subventricular/ventricular zone of adult rat brain.
abstract: Little is known about the response of neural progenitors to inflammation following injuries of the central nervous system. In combination with bromodeoxyuridine (BrdU) intraperitoneally (i.p.) injected, tumor necrosis factor-alpha (TNF-alpha), a proinflammatory cytokine that increased ED1+ activated microglia/macrophage population at injured sites, was administrated into adult rat brains. No difference in the immunostaining for proliferating cell nuclear antigen (PCNA) was observed in the subventricular/ventricular zone (SVZ/VZ) between TNF-alpha injected sites and controls. However, BrdU+ cells were apparently observed in the SVZ/VZ proximal to TNF-alpha injected site, and the number of BrdU+ cells increased at 6 and 24 h post injection. Since cell apoptosis was rarely found in the SVZ/VZ after TNF-alpha injection, these observations suggest that the diffusible TNF-alpha may directly and/or indirectly modulate the proliferation of neural progenitors.
authors.affiliation: Department of Research and Education, Taichung Veterans General Hospital, 40705, Taichung city, Taiwan.
authors.forename: J P
authors.initials: JP
authors.lastname: Wu
authors.forename: J S
authors.initials: JS
authors.lastname: Kuo
authors.forename: Y L
authors.initials: YL
authors.lastname: Liu
authors.forename: S F
authors.initials: SF
authors.lastname: Tzeng
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001921:Brain / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D001973:Bromodeoxyuridine; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002552:Cerebral Ventricles / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D020287:In Situ Nick-End Labeling; D008264:Macrophages / Q000166:cytology; D008297:Male; D017628:Microglia / Q000166:cytology; D008845:Microinjections; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D014409:Tumor Necrosis Factor-alpha; D001973:Bromodeoxyuridine