Immunohistochemical characterization of calf pneumonia produced by the combined endobronchial administration of bovine herpesvirus 1 and Pasteurella haemolytica.
Ten calves ("group 4") were inoculated endobronchially with Pasteurella haemolytica 4 days after inoculation with bovine herpesvirus 1 (BHV-1). Four calves (group 3) were similarly inoculated with P. haemolytica alone, and three (group 2) with BHV-1 alone. All group 4 animals showed severe respiratory signs and had bilateral lobar pneumonia; one died 6 days after inoculation with P. haemolytica. Two types of pneumonic lesion were observed. One was characterized by interlobular and interstitial lymphatic thrombosis, fibrinous pleuritis and coagulative necrosis, and the other by necrotizing bronchiolitis with intranuclear inclusion bodies. The former type of lesion was associated with the presence of P. haemolytica antigen and the latter with the presence of BHV-1 antigen. The weight of infection of BHV-1 and P. haemolytica in bronchoalveolar (BAL) fluid was clearly reflected in the immunohistochemical demonstration of the corresponding antigens in BAL fluid cells. Group 4 calves differed from the calves of groups 1-3 in showing 10-1530 times more endotoxin in BAL fluid. These findings suggested that BHV-1 infection partly destroyed the clearance mechanisms of the respiratory tract epithelium and exacerbated the subsequent P. haemolytica infection.
Narita
M
M
National Institute of Animal Health, Ministry of Agriculture, Forestry and Fisheries, Kannondai, Tsukuba, Ibaraki, 305-0856, Japan.
Kimura
K
K
Tanimura
N
N
Arai
S
S
Tsuboi
T
T
Katsuda
K
K
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D001980:Bronchi / Q000382:microbiology / Q000821:virology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000382:microbiology / Q000821:virology; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology / Q000473:pathology*; D005260:Female; D006566:Herpesviridae Infections / Q000473:pathology / Q000662:veterinary / Q000821:virology; D007242:Herpesvirus 1, Bovine / Q000276:immunology / Q000302:isolation & purification*; D007150:Immunohistochemistry; D008168:Lung / Q000382:microbiology / Q000473:pathology / Q000821:virology; D008297:Male; D016978:Mannheimia haemolytica / Q000276:immunology / Q000302:isolation & purification*; D012766:Pasteurellosis, Pneumonic / Q000382:microbiology / Q000473:pathology*; D011024:Pneumonia, Viral / Q000473:pathology* / Q000821:virology
D000942:Antigens, Bacterial; D000956:Antigens, Viral