title: Interaction of Listeria monocytogenes with the intestinal epithelium.
abstract: Listeria monocytogenes is a food-borne pathogen that must cross the intestinal epithelial barrier to reach its target organs. We have investigated the importance of M cells in translocation using an experimental mouse model and a novel, recently described in vitro coculture system that mimics the follicle-associated epithelium (FAE). Our data demonstrate that L. monocytogenes does not require, nor specifically use, M cells of the FAE to cross the gut. We also show that bacterial translocation is rapid and L. monocytogenes can attach very efficiently to exposed basal lamina of the small intestine indicating an important role for extracellular matrix proteins.
authors.affiliation: Lehrstuhl für Mikrobiologie, Biozentrum der Universität Würzburg, Germany.
authors.forename: J J
authors.initials: JJ
authors.lastname: Daniels
authors.forename: I B
authors.initials: IB
authors.lastname: Autenrieth
authors.forename: W
authors.initials: W
authors.lastname: Goebel
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D018988:Bacterial Translocation; D018938:Caco-2 Cells; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology* / Q000382:microbiology*; D008089:Listeria monocytogenes / Q000472:pathogenicity* / Q000502:physiology; D008088:Listeriosis / Q000382:microbiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D010581:Peyer's Patches / Q000382:microbiology