Molecular cloning of the murine PHEX gene promoter.
We report the novel cloning of the murine PHEX promoter, the gene that is mutated in X-linked hypophosphatemic rickets (XLH). Four promoter/reporter gene constructs, -133/+104, -542/+104, -1061/+104, and -2866/+104, showed significant luciferase activity (4.9-13.2-fold over background) when transfected into rat osteogenic sarcoma (UMR-106) cells.
Hines
E R
ER
Department of Pediatrics, Steele Memorial Children's Research Center, University of Arizona Health Sciences Center, 1501 N. Campbell Avenue, Tucson, AZ 85724, USA.
Collins
J F
JF
Ghishan
F K
FK
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D008156:Luciferases / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D053402:PHEX Phosphate Regulating Neutral Endopeptidase; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D051381:Rats; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D011506:Proteins; D008156:Luciferases; D053402:PHEX Phosphate Regulating Neutral Endopeptidase; C506232:Phex protein, mouse; C506229:Phex protein, rat