title: Aeromonas hydrophila isolated from wild freshwater fish in Croatia.
abstract: Aeromonas hydrophila was recovered from fish living in lake Vrana on the Croatian island of Cres. The occurrence of the bacterium in the fish was assessed and related to gross signs of disease and findings at necropsy as a potential health hazard for fish. Isolated bacteria were subjected to morphological, physiological, biochemical and antibiotic susceptibility tests. A total of 26 A. hydrophila isolates were obtained. There was a clear seasonality, as no isolates were recovered in the summer months. Most of the isolates were sensitive to all the antimicrobials used in the study except novobiocin and penicillin G. Affected fish manifested haemorrhages over the skin, in the liver, kidney and swim bladder, spleen infarcts, fatty liver, ascitic fluid and swollen haemopoietic tissues. A. hydrophila does not appear to pose a major threat for the fish in the lake at present but under unfavourable and stressful conditions it could seriously compromise fish health.
authors.affiliation: Ruder Boskovic Institute, Centre for Marine and Environmental Research, Laboratory for Aquaculture, Zagreb, Croatia. ntopic@rudjer.irb.hr
authors.forename: N
authors.initials: N
authors.lastname: Topić Popovic
authors.forename: E
authors.initials: E
authors.lastname: Teskeredzić
authors.forename: I
authors.initials: I
authors.lastname: Strunjak-Perović
authors.forename: R
authors.initials: R
authors.lastname: Coz-Rakovac
mesh_terms: D016980:Aeromonas hydrophila / Q000737:chemistry / Q000302:isolation & purification* / Q000472:pathogenicity; D000818:Animals; D002374:Catalase / Q000032:analysis; D019343:Citric Acid / Q000378:metabolism; D017523:Croatia; D003530:Cyprinidae; D004352:Drug Resistance, Microbial; D017859:Esocidae; D005393:Fish Diseases / Q000382:microbiology*; D005618:Fresh Water; D018093:Gelatinases / Q000032:analysis; D005880:Gills / Q000382:microbiology / Q000473:pathology; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology / Q000662:veterinary*; D006862:Hydrogen Sulfide / Q000032:analysis; D007668:Kidney / Q000382:microbiology / Q000473:pathology; D008099:Liver / Q000382:microbiology / Q000473:pathology; D010088:Oxidoreductases / Q000032:analysis; D013154:Spleen / Q000382:microbiology / Q000473:pathology
chemical_list: D019343:Citric Acid; D010088:Oxidoreductases; D002374:Catalase; D018093:Gelatinases; D006862:Hydrogen Sulfide