Maternal cold inducible RNA binding protein is required for embryonic kidney formation in Xenopus laevis.
We cloned a major isoform of Xenopus homologue of cold inducible RNA binding protein (CIRP), XCIRP-1. XCIRP-1 was neither cold inducible nor essential for cell division during early embryonic development. Suppression of XCIRP-1 dose dependently produced tailbuds with deformations of the brain and internal organs. The defects were XCIRP-1 specific as they could be rescued by sense transcript. Suppression of XCIRP-1 also disrupted the morphogenetic migration of the C3 blastomeres (lineaged to become the embryonic kidney, the pronephros). In animal cap explants, depletion of XCIRP-1 inhibited activin/retinoic acid induced expressions of pronephros related Xlim-1 and WT1 genes. These results suggest that XCIRP-1 is required for the specification and morphogenetic lineage migration of the pronephros.
Peng
Y
Y
Department of Neurology, The First Military Medical University of China, Guangzhou, PR China.
Kok
K H
KH
Xu
R H
RH
Kwok
K H
KH
Tay
D
D
Fung
P C
PC
Kung
H F
HF
Lin
M C
MC
D000015:Abnormalities, Multiple / Q000235:genetics; D000818:Animals; D002455:Cell Division; D003001:Cloning, Molecular; D004625:Embryo, Nonmammalian / Q000502:physiology; D019143:Evolution, Molecular; D005260:Female; D007668:Kidney / Q000166:cytology / Q000196:embryology*; D009024:Morphogenesis; D010641:Phenotype; D010802:Phylogeny; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013489:Suppression, Genetic; D014158:Transcription, Genetic; D029867:Xenopus Proteins; D014982:Xenopus laevis / Q000196:embryology* / Q000235:genetics
C491389:CIRP2 protein, Xenopus; D020033:Protein Isoforms; D016601:RNA-Binding Proteins; D029867:Xenopus Proteins