title: Incorporation and metabolism of dietary trans isomers of linolenic acid alter the fatty acid profile of rat tissues.
abstract: To study the influence on lipid metabolism and platelet aggregation of the fatty acid isomerization that occurs during heat treatment, weanling rats were fed for 8 wk a diet enriched with 5% isomerized (experimental group) or normal (control group) canola oil. Geometrical isomers of alpha-linolenic acid representing 0.2 g/100 g of the experimental diet were incorporated into liver, platelets, aorta and heart, at the expense of their cis homologue and of 18:2(n-6). The major isomer, 9c,12c,15t-18:3, was also metabolized to 5c,8c,11c,14c,17t-20:5 and to an unknown compound, found in liver, platelets and aorta, which has been identified tentatively as 7c, 10c,13c,16c,19t-22:5. The greater 20:4(n-6)/18:2(n-6) ratio in the liver, platelets and heart of the experimental group than the control group indicated an enhancement of desaturation activities. This induced a higher content of long-chain (n-6) fatty acids in the experimental group. Platelet aggregation tended to be slightly higher (P: = 0.065) in the experimental group. We conclude that 0.2 g of trans isomers of alpha-linolenic acid per 100 g of diet was sufficient to be incorporated and metabolized, thus altering the fatty acid profile of rat tissues.
authors.affiliation: INRA, Unité de Nutrition Lipidique, BP 86510-21605 Dijon, France and. INRA, Laboratoire de Recherche sur les Arômes, 21000 Dijon, France.
authors.forename: C
authors.initials: C
authors.lastname: Loï
authors.forename: J M
authors.initials: JM
authors.lastname: Chardigny
authors.forename: S
authors.initials: S
authors.lastname: Almanza
authors.forename: L
authors.initials: L
authors.lastname: Leclere
authors.forename: C
authors.initials: C
authors.lastname: Ginies
authors.forename: J L
authors.initials: JL
authors.lastname: Sébédio
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000378:metabolism; D001835:Body Weight; D003094:Collagen / Q000494:pharmacology; D004042:Dietary Fats, Unsaturated / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism*; D005229:Fatty Acids, Monounsaturated / Q000008:administration & dosage; D007536:Isomerism; D008099:Liver / Q000033:anatomy & histology / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism; D010974:Platelet Aggregation / Q000187:drug effects; D000074262:Rapeseed Oil; D051381:Rats; D017208:Rats, Wistar; D014886:Weaning; D017962:alpha-Linolenic Acid / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D004042:Dietary Fats, Unsaturated; D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D000074262:Rapeseed Oil; D017962:alpha-Linolenic Acid; D003094:Collagen