title: Effects of CO2 insufflation and laparotomy on wound healing in mice.
abstract: BACKGROUND
  The aim of the present study was to evaluate the effects of laparotomy and CO2 insufflation on wound healing in a murine incisional wound healing model.
  
  
  METHODS
  Seventy-two male Swiss Albino mice were randomly allocated into three groups of control, laparotomy and CO2 insufflation. A transverse skin incision of 15 mm was made in the dorsum of each mouse, and four interrupted mattress sutures with 4.0 polypropylene thread were laid for wound closure. A median laparotomy was performed in the laparotomy group. CO2 insufflation was performed with an intra-abdominal pressure of 9 mmHg. The retained gas was evacuated from the abdominal cavity at the end of a 60-min period. Mice were killed on the 3rd, 7th and 15th postoperative days. The wound tensile strength and 5-hydroxyproline concentration in the wound tissue were measured.
  
  
  RESULTS
  Tensile strength of the incised skin increased as the post-incision period progressed. There was no significant difference between the tensile strengths of the incised skin of control, laparotomy and CO2 insufflation groups throughout the observation period. The skin 5-hydroxyproline concentrations of all groups were not significantly different at the 3rd postoperative day. But laparotomy and CO2 insufflation groups had lower 5-hydroxyproline concentrations at the 7th and 15th postoperative days, when compared to controls (P < 0.02 for 7th and 15th days).
  
  
  CONCLUSIONS
  CO2 insufflation and laparotomy reduce the 5-hydroxyproline concentration of the wound, suggesting a diminished wound healing capacity.
authors.affiliation: Department of General Surgery, Suleyman Demirel University Faculty of Medicine, Isparta, Turkey. fatihagalar@superonline.com
authors.forename: F
authors.initials: F
authors.lastname: Agalar
authors.forename: E
authors.initials: E
authors.lastname: Hamaloglu
authors.forename: C
authors.initials: C
authors.lastname: Daphan
authors.forename: A
authors.initials: A
authors.lastname: Tarim
authors.forename: R
authors.initials: R
authors.lastname: Onur
authors.forename: N
authors.initials: N
authors.lastname: Renda
authors.forename: I
authors.initials: I
authors.lastname: Sayek
mesh_terms: D000818:Animals; D002245:Carbon Dioxide; D007327:Insufflation; D010535:Laparoscopy; D007813:Laparotomy; D008297:Male; D051379:Mice; D013718:Tensile Strength; D014945:Wound Healing / Q000502:physiology*
chemical_list: D002245:Carbon Dioxide