Packaging of small molecules into VP1-virus-like particles of the human polyomavirus JC virus.
Recombinantly expressed VP1-virus-like particles (VP1-VLP) of human polyomavirus JC virus (JCV) were described recently as a new DNA transporter system. It was shown that DNA molecules could be packaged into VP1-VLP during a controlled chemical reassociation/dissociation process. In the present study VP1-VLP were studied as carriers for pharmaceutical substances. Propidium iodide (PI) was packaged into VP1-VLP as a reporter molecule. The PI-containing VP1-VLP could be detected directly by flow cytometry. The fluorescence intensity of the VP1-VLP depended strongly on the initial PI concentration. This packaging method is easy to handle and applicable to viruses and VP1-VLP which can be dissociated and reassociated chemically.
Goldmann
C
C
Department of Virology and Immunology, German Primate Centre, Kellnerweg 4, 37077, Göttingen, Germany. cgoldm@www.dpz.gwdg.de
Stolte
N
N
Nisslein
T
T
Hunsmann
G
G
Lüke
W
W
Petry
H
H
D002213:Capsid / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D036022:Capsid Proteins; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D016503:Drug Delivery Systems; D005434:Flow Cytometry; D006801:Humans; D007577:JC Virus / Q000378:metabolism*; D008854:Microscopy, Electron; D011419:Propidium / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014771:Virion / Q000235:genetics / Q000378:metabolism*; D019065:Virus Assembly
D036022:Capsid Proteins; D011993:Recombinant Fusion Proteins; C062481:VP1 protein, polyomavirus; D011419:Propidium; D004247:DNA