Analysis of dipeptidyl peptidase IV gene regulation in transgenic mice: DNA elements sufficient for promoter activity in the kidney, but not the intestine, reside on the proximal portion of the gene 5'-flanking region.
The dipeptidyl peptidase IV (DPPIV) gene encodes a brush border membrane exopeptidase that is expressed in a tissue-restricted fashion. To examine the regulation of DPPIV transcription in various tissues in vivo, we examined the expression of DPPIV 5'-flanking region (promoter)-human growth hormone reporter constructs in transgenic mice. These mice exhibited cell-type specific reporter expression in kidney. Surprisingly, however, only very low to non-detectable levels of reporter were found in small intestine. These results indicate that DNA elements sufficient for DPPIV expression in kidney, but not intestine, reside in the 5'-flanking region of the gene.
Gum
J R
JR
Gastrointestinal Research Laboratory, Department of Veterans' Affairs Medical Center, San Francisco, CA 94121, USA. jgum@maelstrom.ucsf.edu
Erickson
R H
RH
Hicks
J W
JW
Rius
J L
JL
Kim
Y S
YS
D020121:5' Untranslated Regions / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D018819:Dipeptidyl Peptidase 4 / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D017930:Genes, Reporter; D019382:Human Growth Hormone / Q000235:genetics; D006801:Humans; D007421:Intestine, Small / Q000201:enzymology*; D007668:Kidney / Q000201:enzymology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic
D020121:5' Untranslated Regions; D012333:RNA, Messenger; D019382:Human Growth Hormone; D018819:Dipeptidyl Peptidase 4