title: Temporal regulation of endothelial ET-1 and eNOS expression in intact human conduit vessels exposed to different intraluminal pressure levels at physiological shear stress.
abstract: OBJECTIVE
  By using a computerized vascular perfusion model, we investigated temporal effects of sub-acute pressure elevation on vasomotor behavior and expression of endothelin-1 (ET-1) and endothelial nitric oxide synthase (eNOS) in intact human conduit vessels.
  
  
  METHODS
  Paired umbilical veins were perfused during 1.5, 3 and 6 h under high/low intraluminal pressure (40/20 mmHg) and at identical shear stress level of 10 dyn/cm(2). ET-1 and eNOS gene and protein expression was quantified with real-time reverse-transcribed polymerase chain reaction and quantitative immunohistochemistry, respectively.
  
  
  RESULTS
  Pressure induced differential temporal regulation patterns of ET-1 and eNOS gene expression. During the high pressure condition, eNOS mRNA was upregulated after 3 h and leveled off after 6 h of perfusion, while ET-1 mRNA was elevated after 6 h perfusion. Immunohistochemistry verified synchronal changes at the protein level. Significant vasodilation was observed after 3 h in the high-pressure system.
  
  
  CONCLUSIONS
  Thus, subacute pressure elevation exerts differential effects on the endothelial eNOS/ET-1 expression, which dynamically regulate the vasomotor tone.
authors.affiliation: Clinical Experimental Research Laboratory, Heart and Lung Institute, Sahlgrenska University Hospital/Ostra, Göteborg University, SE 416 85, Gothenburg, Sweden.
authors.forename: L M
authors.initials: LM
authors.lastname: Gan
authors.forename: L
authors.initials: L
authors.lastname: Selin-Sjögren
authors.forename: R
authors.initials: R
authors.lastname: Doroudi
authors.forename: S
authors.initials: S
authors.lastname: Jern
mesh_terms: D000704:Analysis of Variance; D003198:Computer Simulation; D019332:Endothelin-1 / Q000032:analysis / Q000235:genetics*; D004730:Endothelium, Vascular / Q000737:chemistry / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D008955:Models, Cardiovascular; D019001:Nitric Oxide Synthase / Q000032:analysis / Q000235:genetics*; D052250:Nitric Oxide Synthase Type III; D010477:Perfusion; D011312:Pressure; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014655:Vascular Resistance
chemical_list: D019332:Endothelin-1; C496314:NOS3 protein, human; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III