A low-molecular-weight cytosolic inhibitor of the specific testosterone binding in bovine seminal vesicles.
Bovine seminal vesicle cytosol contains a low-molecular-weight and thermostable substance which specifically inhibits the binding of testosterone to its cognate receptor. The mass and the ether phospholipid structure of the inhibitor were elucidated by mass spectrometry. Saturation and binding experiments indicate that the inhibitor acts in a dose-dependent and competitive manner, altering the apparent dissociation constant (K(D)) while maintaining the number of androgen-binding sites (B(max)). Its possible role in the regulation of androgen binding activity is discussed.
Oberste-Frielinghaus
H R
HR
Institute of Physiological Chemistry, Eberhard-Karls University of Tübingen, Hoppe-Seyler Strasse 4, Tübingen, 72076, Germany.
Burger
M
M
Rapior
N
N
Taylor
H V
HV
Eisele
K
K
D000818:Animals; D002417:Cattle; D002855:Chromatography, Thin Layer; D003600:Cytosol / Q000378:metabolism; D003956:Dialysis; D004305:Dose-Response Relationship, Drug; D006727:Hormone Antagonists / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D013058:Mass Spectrometry; D008970:Molecular Weight; D012669:Seminal Vesicles / Q000378:metabolism*; D013739:Testosterone / Q000037:antagonists & inhibitors* / Q000378:metabolism; D014316:Tritium
D006727:Hormone Antagonists; D014316:Tritium; D013739:Testosterone