Molecular cloning of testis-abundant finger Protein/Ring finger protein 23 (RNF23), a novel RING-B box-coiled coil-B30.2 protein on the class I region of the human MHC.
We have identified a genomic DNA fragment, using the PCR method with degenerate oligonucleotide primers which contain the conserved sequence of the RING finger domain. Using the DNA fragment as a probe, a novel cDNA was cloned from human and mouse testis. The cDNA had a domain structure of the typical RING-B box-coiled coil (RBCC)-B30.2 domain and therefore was named testis-abundant finger protein (tfp). Indeed, the transcript was highly expressed in the testis, although it was also found ubiquitously in various organs by Northern blot analysis. The tfp gene was mapped at the class I region of the human MHC (major histocompatibility complex), within which some known RBCC-B30.2 proteins such as RFP, RFB30/HERF1, AFP, and HZF had been localized. These findings demonstrate that several RBCC-B30.2 proteins including tfp, which are non-HLA proteins, are clustered within the class I region of the human MHC.
Orimo
A
A
Department of Biochemistry, Institute of Laboratory Animal Science, Saitama Medical School, 38 Moro-Hongo, Moroyama-machi, Iruma-gun, Saitama, 350-0495, Japan. aorimo@saitama-med.ac.jp
Yamagishi
T
T
Tominaga
N
N
Yamauchi
Y
Y
Hishinuma
T
T
Okada
K
K
Suzuki
M
M
Sato
M
M
Nogi
Y
Y
Suzuki
H
H
Inoue
S
S
Yoshimura
K
K
Shimizu
Y
Y
Muramatsu
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D005805:Genes, MHC Class I; D016678:Genome; D015894:Genome, Human; D006649:Histocompatibility Antigens / Q000235:genetics; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D013737:Testis; D000070584:Tripartite Motif Proteins; D044767:Ubiquitin-Protein Ligases
D002352:Carrier Proteins; D006649:Histocompatibility Antigens; D047908:Intracellular Signaling Peptides and Proteins; C108239:TRIM10 protein, human; D000070584:Tripartite Motif Proteins; C414583:TRIM39 protein, human; D044767:Ubiquitin-Protein Ligases