A monoclonal antibody against the 66-kDa protein expressed in mouse spleen and thymus inhibits Ly-6A.2-dependent cell-cell adhesion.
The Ly-6 locus encodes several cell surface proteins of 10-12 kDa. Some members of this multigene family may function in cell signaling and/or cell adhesion processes. T lymphocytes overexpressing Ly-6A.2 (one member of the Ly-6 gene family) protein homotypically aggregate when cultured in vitro. Further analysis of this homotypic aggregation suggests that Ly-6A.2 participates in cell-cell adhesion. These observations indicated the presence of a Ly-6 ligand(s) on the surface of lymphoid cells. In this study we report generation of a hamster mAb, 9AB2, that blocks Ly-6A.2-dependent cell-cell adhesion. The 9AB2 Ab recognizes a 66-kDa glycoprotein with unique tissue expression. The 9AB2 mAb does not bind Ly-6A.2, but coimmunoprecipitates Ly-6A.2 molecule. Moreover, 9AB2 Ag-expressing thymocytes specifically bind to Chinese hamster ovary cells overexpressing Ly-6A.2 protein, and this binding is specifically blocked by 9AB2 and anti-Ly-6A.2 Abs. These results suggest that the 66-kDa protein recognized by 9AB2 mAb is the putative ligand for Ly-6A.2.
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Department of Cellular Biology University of Georgia, Athens 30602, USA.
Kosoy
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Pawlinski
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Bamezai
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D016475:3T3 Cells; D000818:Animals; D019138:Antibodies, Blocking / Q000096:biosynthesis / Q000378:metabolism / Q000494:pharmacology; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000378:metabolism / Q000494:pharmacology*; D000950:Antigens, Ly / Q000502:physiology*; D001666:Binding Sites, Antibody; D016466:CHO Cells; D002448:Cell Adhesion / Q000276:immunology; D002449:Cell Aggregation / Q000276:immunology; D002460:Cell Line; D002461:Cell Line, Transformed; D006224:Cricetinae; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008024:Ligands; D008565:Membrane Proteins / Q000037:antagonists & inhibitors* / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D008970:Molecular Weight; D009928:Organ Specificity / Q000235:genetics / Q000276:immunology; D014176:Protein Biosynthesis; D011506:Proteins / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D013154:Spleen / Q000276:immunology* / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D019076:Transgenes / Q000276:immunology; D014407:Tumor Cells, Cultured
D019138:Antibodies, Blocking; D000911:Antibodies, Monoclonal; D000950:Antigens, Ly; D007166:Immunosuppressive Agents; D008024:Ligands; C074650:Ly6a protein, mouse; D008565:Membrane Proteins; D011506:Proteins