title: Effect of neocuproine, a selective Cu(I) chelator, on nitrergic relaxations in the mouse corpus cavernosum.
abstract: The effects of neocuproine and bathocuproine, Cu(I) and Cu(II) chelators, respectively, were studied on relaxations in response to electrical field stimulation, acetylcholine, S-nitrosoglutathione, acidified sodium nitrite and sodium nitroprusside in the mouse corpus cavernosum precontracted with phenylephrine. Neocuproine significantly inhibited relaxations induced by electrical field stimulation, acetylcholine and S-nitrosoglutathione, but not by acidified sodium nitrite and sodium nitroprusside. The pre-prepared neocuproine-Cu(I) complex was ineffective on the responses. The discrepancy between the shape of relaxations in response to electrical field stimulation or to acetylcholine and S-nitrosoglutathione was abolished by adding CuCl(2) into the bathing medium. The copper action was blocked by neocuproine but not by bathocuproine. However, the pre-prepared bathocuproine-Cu(II) complex did not accelerate the relaxations affected by CuCl(2). These findings suggest that a Cu(I)-dependent mechanism may play a role in the relaxation induced by the endogenous relaxant factor as well as by S-nitrosoglutathione in mouse cavernosal tissue.
authors.affiliation: Medical Faculty, Department of Pharmacology, Cukurova University, TR-01330, Adana, Turkey. gocmencc@hotmail.com
authors.forename: C
authors.initials: C
authors.lastname: Göçmen
authors.forename: H S
authors.initials: HS
authors.lastname: Göktürk
authors.forename: P U
authors.initials: PU
authors.lastname: Ertuğ
authors.forename: S
authors.initials: S
authors.lastname: Onder
authors.forename: A
authors.initials: A
authors.lastname: Dikmen
authors.forename: F
authors.initials: F
authors.lastname: Baysal
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D002614:Chelating Agents / Q000494:pharmacology*; D003300:Copper / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D005978:Glutathione / Q000031:analogs & derivatives / Q000494:pharmacology; D008297:Male; D051379:Mice; D009569:Nitric Oxide / Q000502:physiology*; D009599:Nitroprusside / Q000494:pharmacology; D009603:Nitroso Compounds / Q000494:pharmacology; D010413:Penis / Q000187:drug effects* / Q000502:physiology; D010618:Phenanthrolines / Q000494:pharmacology*; D010656:Phenylephrine / Q000494:pharmacology; D026422:S-Nitrosoglutathione
chemical_list: D002614:Chelating Agents; D009603:Nitroso Compounds; D010618:Phenanthrolines; D009599:Nitroprusside; D010656:Phenylephrine; D009569:Nitric Oxide; D026422:S-Nitrosoglutathione; D003300:Copper; C002701:neocuproine; C002478:bathocuproine; D005978:Glutathione; D000109:Acetylcholine