Dietary fructans modulate polyamine concentration in the cecum of rats.
Nondigestible but fermentable dietary fructans such as oligofructose exert many effects on gut physiology through their fermentation end products such as short-chain fatty acids. Could other metabolites be produced in the gut and contribute to the physiologic effects of dietary fructans? The aim of the study was to evaluate the influence of oligofructose on putrescine, spermidine and spermine concentrations in the cecum, the portal vein and the liver of rats and to assess their involvement in cecal enlargement and the modulation of hepatic lipid metabolism. Putrescine, spermidine and spermine were quantified by HPLC in samples obtained from male Wistar rats fed a nonpurified standard diet (controls) or the same diet enriched with 10 g/100 g oligofructose (OFS) for 4 wk. OFS-fed rats had significantly greater cecal content and tissue weights. OFS almost doubled the concentration of putrescine in the cecal contents. The concentration of all three polyamines in the cecal tissue was significantly greater than in controls. The concentration of spermidine in portal plasma was lower in rats fed OFS, whereas the treatment did not affect the polyamine concentrations in the liver. The fermentation of dietary fructans contributed to an increase in the concentration of putrescine in the gut without modifying putrescine concentration in either the portal blood or liver. Moreover, the greater levels of polyamines in cecal tissue may be related to the cell proliferation resulting from OFS fermentation in the gut.
Delzenne
N M
NM
Unit of Pharmacokinetics, Metabolism, Nutrition and Toxicology PMNT 7369 Université Catholique de Louvain, B-1200 Brussels, Belgium.
Kok
N
N
Deloyer
P
P
Dandrifosse
G
G
D000818:Animals; D002432:Cecum / Q000737:chemistry / Q000378:metabolism*; D004040:Dietary Carbohydrates / Q000494:pharmacology*; D005630:Fructans / Q000494:pharmacology*; D008297:Male; D009844:Oligosaccharides / Q000008:administration & dosage / Q000494:pharmacology; D011073:Polyamines / Q000032:analysis / Q000378:metabolism*; D011700:Putrescine / Q000097:blood / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013095:Spermidine / Q000097:blood / Q000378:metabolism; D013096:Spermine / Q000097:blood / Q000378:metabolism
D004040:Dietary Carbohydrates; D005630:Fructans; D009844:Oligosaccharides; D011073:Polyamines; C120489:oligofructose; D013096:Spermine; D013095:Spermidine; D011700:Putrescine