title: The efficacy of a broad-spectrum sunscreen to protect engineered human skin from tissue and DNA damage induced by solar ultraviolet exposure.
abstract: Sunscreens are known to protect against sunlight-induced erythema and sunburn, but their efficiency at protecting against skin cancer is still a matter of debate. Specifically, the capacity of sunscreens to prevent or reduce tissue and DNA damage has not been thoroughly investigated. The present study was undertaken to assess the ability of a chemical broad-spectrum sunscreen to protect human skin against tissue and DNA damage after solar UV radiation. Engineered human skin was generated and either treated or not with a broad-spectrum SPF 30 sunscreen and exposed to increasing doses of simulated sunlight (SSL). Immediately after irradiation, histological, immunohistochemical, and molecular quantitative analyses were performed. The unprotected irradiated engineered human skin showed significant epidermal disorganization accompanied by a complete absence of laminin deposition. The sunscreen prevented SSL-induced epidermal damage at low doses and allowed laminin deposition at almost all SSL doses tested. The frequencies of cyclobutane pyrimidine dimers, pyrimidine (6-4) pyrimidone photoproducts, and photooxidative lesions measured by alkaline gel electrophoresis and radioimmunoassay were significantly reduced by the sunscreen. Thus, tissue and DNA damage may provide excellent quantitative end points for assessing the photoprotective efficacy of sunscreens.
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Laval University, Centre de Recherche, Hôpital Saint-François d'Assise, CHUQ, Quebec, Canada.
authors.forename: V
authors.initials: V
authors.lastname: Bissonauth
authors.forename: R
authors.initials: R
authors.lastname: Drouin
authors.forename: D L
authors.initials: DL
authors.lastname: Mitchell
authors.forename: M
authors.initials: M
authors.lastname: Rhainds
authors.forename: J
authors.initials: J
authors.lastname: Claveau
authors.forename: M
authors.initials: M
authors.lastname: Rouabhia
mesh_terms: D000293:Adolescent; D000328:Adult; D002478:Cells, Cultured; D003503:Cyclobutanes / Q000378:metabolism; D004249:DNA Damage; D019281:Dimerization; D004307:Dose-Response Relationship, Radiation; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000378:metabolism; D007797:Laminin / Q000378:metabolism; D011743:Pyrimidines / Q000378:metabolism; D011744:Pyrimidinones / Q000378:metabolism; D011863:Radioimmunoassay; D012867:Skin / Q000187:drug effects* / Q000528:radiation effects*; D013473:Sunscreening Agents / Q000627:therapeutic use*; D014466:Ultraviolet Rays
chemical_list: D003503:Cyclobutanes; D007797:Laminin; D011743:Pyrimidines; D011744:Pyrimidinones; D013473:Sunscreening Agents; C030986:pyrimidine