title: The effect of super-oxidized water on Escherichia coli.
abstract: The mechanism of action of Sterilox, a non-toxic liquid biocide produced by electrolysis of a dilute saline solution, upon planktonic cells of Escherichia coli JM109 was investigated using protein and nucleic acid analysis. The results revealed total destruction of chromosomal and plasmid DNA, RNA and proteins of E. coli within 5 min of exposure. Our earlier investigation conducted using atomic force microscopy imaging revealed swelling and rupture of E. coli cells with release of cytoplasm. We propose that the biocidal properties of Sterilox are due to its effect upon constituents of the bacterial cell including proteins and nucleic acids.
authors.affiliation: School of Pharmacy and Biomedical Sciences, University of Portsmouth, St. Michael's Building, White Swan Road, Portsmouth, PO1 2DT, UK. vitaly.zinkevich@port.ac.uk
authors.forename: V
authors.initials: V
authors.lastname: Zinkevich
authors.forename: I B
authors.initials: IB
authors.lastname: Beech
authors.forename: R
authors.initials: R
authors.lastname: Tapper
authors.forename: I
authors.initials: I
authors.lastname: Bogdarina
mesh_terms: D001426:Bacterial Proteins / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects; D004269:DNA, Bacterial / Q000187:drug effects; D004202:Disinfectants; D004353:Drug Evaluation, Preclinical; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000145:classification / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D006861:Hydrogen Peroxide; D018625:Microscopy, Atomic Force; D016877:Oxidants; D010084:Oxidation-Reduction; D010957:Plasmids / Q000187:drug effects; D012329:RNA, Bacterial / Q000187:drug effects; D012703:Serotyping
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004202:Disinfectants; D016877:Oxidants; D012329:RNA, Bacterial; C118085:superoxidized water; D006861:Hydrogen Peroxide