[Inhibitory effect of 7 agents on bovine testis aldose reductase].
The inhibitory effects of seven agents on aldose reductase were studied by enzymatic kinetics using the purified enzyme from bovine testis, sorbinil, tolrestat, silybin and berberini were found to exhibit a marked enzyme-inhibitory effect with Ki values of 1.08 mumol/L, 0.12 mumol/L, 3.5 micrograms/ml, and 2.7 micrograms/ml respectively. The established assay system described in this paper may be useful in screening aldose reductase inhibitors.
Liu
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C
Huashan Hospital of Shanghai Medical University.
Zhu
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D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors* / Q000302:isolation & purification; D000818:Animals; D002417:Cattle; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D007093:Imidazoles / Q000494:pharmacology; D048289:Imidazolidines; D008297:Male; D009281:Naphthalenes / Q000494:pharmacology; D012838:Silymarin / Q000494:pharmacology; D013737:Testis / Q000201:enzymology*
D004791:Enzyme Inhibitors; D007093:Imidazoles; D048289:Imidazolidines; D009281:Naphthalenes; D012838:Silymarin; C040550:tolrestat; D000449:Aldehyde Reductase; C026411:sorbinil