title: Effect of progesterone on the activation of neurones of the supraoptic nucleus during parturition.
abstract: Parturition is driven by a pulsatile pattern of oxytocin secretion, resulting from burst firing activity of supraoptic oxytocin neurones and reflected by induction of Fos expression. Rats were injected with progesterone on day 20 of pregnancy to investigate the role of the decreasing progesterone:ratio oestrogen ratio, which precedes delivery, in the activation of supraoptic neurones. Progesterone delayed the onset of birth by 28 h compared with vehicle (control) and prolonged the duration of delivery, which was overcome by pulsatile injections of oxytocin, indicating that the slow delivery may reflect impaired oxytocin secretion. Parturient rats pretreated with progesterone had fewer Fos immunoreactive nuclei in the supraoptic nucleus than did parturient rats pretreated with vehicle. The number of Fos immunoreactive nuclei was not restored after oxytocin injection, indicating that appropriate activation of oxytocin neurones is impaired by progesterone and also that there is a lack of stimulatory afferent drive. Fos expression increased in the nucleus of the tractus solitarius during parturition in rats pretreated with either vehicle or progesterone, but not in rats that had been pretreated with progesterone and induced with oxytocin, indicating that this input was inhibited. Endogenous opioids inhibit oxytocin neurones in late pregnancy and the opioid antagonist, naloxone, increases Fos expression in supraoptic nuclei by preventing inhibition. However, progesterone attenuated naloxone-induced Fos expression in the supraoptic nucleus in late pregnancy and naloxone administered during parturition did not accelerate the duration of births delayed by progesterone administration, indicating that progesterone does not act by hyperactivation of endogenous opioid tone. RU486, a progesterone receptor antagonist, enhanced supraoptic neurone Fos expression in late pregnancy, indicating progesterone receptor-mediated actions. Thus, progesterone withdrawal is necessary for appropriate activation of supraoptic and tractus solitarius neurones during parturition.
authors.affiliation: Department of Psychiatry, Max Planck Institute of Psychiatry, Munich, Germany.
authors.forename: I A
authors.initials: IA
authors.lastname: Antonijevic
authors.forename: J A
authors.initials: JA
authors.lastname: Russell
authors.forename: R J
authors.initials: RJ
authors.lastname: Bicknell
authors.forename: G
authors.initials: G
authors.lastname: Leng
authors.forename: A J
authors.initials: AJ
authors.lastname: Douglas
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D003864:Depression, Chemical; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D016762:Genes, fos; D006727:Hormone Antagonists / Q000494:pharmacology; D007150:Immunohistochemistry; D007743:Labor, Obstetric / Q000187:drug effects*; D015735:Mifepristone / Q000494:pharmacology; D009270:Naloxone / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects; D010121:Oxytocin / Q000494:pharmacology; D011247:Pregnancy; D011374:Progesterone / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013495:Supraoptic Nucleus / Q000187:drug effects*
chemical_list: D006727:Hormone Antagonists; D009292:Narcotic Antagonists; D015735:Mifepristone; D009270:Naloxone; D011374:Progesterone; D010121:Oxytocin