title: [cDNA cloning, sequence analysis of trehalose-6-phosphate synthase gene from Saccharomyces cerevisiae AS2.1416].
abstract: The trehalose-6-phosphate synthase gene tps1 was amplified from yeast S. cerevisiae AS2.1416 cDNA library by polymerase chain reaction. This 1.5 kb fragment was cloned into PstI and BamHI sites of pGEM-T easy vector and the sequence of the gene indicated the cloned tps1 gene contained 1485 nucleotides encoding for 495 amino acid and shared a sequence homology of 99.6% with that from S. cerevisiae S288C.
authors.affiliation: Enzymology Department, Chinese Academy of Sciences, Beijing.
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Dong
authors.forename: Y K
authors.initials: YK
authors.lastname: Duan
authors.forename: H M
authors.initials: HM
authors.lastname: Chen
authors.forename: C
authors.initials: C
authors.lastname: Jin
authors.forename: S Z
authors.initials: SZ
authors.lastname: Zhang
mesh_terms: D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D015723:Gene Library; D005964:Glucosyltransferases / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D005964:Glucosyltransferases; C023180:trehalose-6-phosphate synthase