[Construction of a transformation-competent artificial chromosome (TAC) library of a wheat-Haynaldia villosa translocation line].
Transformation-competent artificial chromosome (TAC) vector is able to clone and transfer large DNA fragments in plants and is a powerful tool for plant gene isolation and transformation. To clone important genes from wheat, a TAC genomic library was constructed from nuclear DNA of a 6VS/6AL wheat-Haynaldia villosa translocation line that harbor the gene Pm21 for resistance to powdery mildew. The library consists of 2.1 x 10(6) clones with an average DNA insert size of 35 kb, and represents in total 4.9 genome equivalents. The library was stored as clone pools in 96-well plates, and each pool contained about 1000 clones. TAC clones containing gene(s) of interest can be screened by a pooled-PCR/colony-hybridization strategy.
Fang
Y D
YD
Key Laboratory of Crop Cytogenetics, Minstry of Agricultural, Nanjing Agricultural University.
Liu
Y G
YG
Wu
H
H
Zhang
Q Y
QY
Chen
P D
PD
Liu
D J
DJ
D022201:Chromosomes, Artificial; D015723:Gene Library; D014170:Transformation, Genetic; D014178:Translocation, Genetic; D014908:Triticum / Q000235:genetics*