Protective effect of beta-carbolines and other antioxidants on lipid peroxidation due to hydrogen peroxide in rat brain homogenates.
Tryptoline and pinoline are two beta-carbolines isolated from the nervous system of mammals. We investigated the ability of these compounds to prevent lipid peroxidation induced by hydrogen peroxide in rat brain homogenates. We also compared their effects with other known antioxidants including melatonin, trolox and ascorbic acid. Lipid peroxidation was assessed by measuring malonaldehyde (MDA) and 4-hydroxy-alkenals (4-HDA) concentrations in the brain homogenates. Incubation with hydrogen peroxide (5 mM) increased MDA+4-HDA levels, which were totally prevented by tryptoline, pinoline, melatonin and trolox in a concentration-dependent manner. By contrast, higher MDA-4-HDA concentrations compared with control experiments were found after incubation with ascorbic acid, thus reflecting an increase of lipid peroxidation induced by this compound. Although in vivo studies are needed, the data suggest that these beta-carbolines may be potential neuroprotective agents because of their antioxidant activities.
García
J J
JJ
Department of Pharmacology and Physiology, University of Zaragoza, Zaragoza, Spain.
Martínez-Ballarín
E
E
Robinson
M
M
Allué
J L
JL
Reiter
R J
RJ
Osuna
C
C
Acuña-Castroviejo
D
D
D000447:Aldehydes / Q000032:analysis / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000378:metabolism* / Q000494:pharmacology; D001205:Ascorbic Acid / Q000378:metabolism / Q000494:pharmacology; D001923:Brain Chemistry / Q000187:drug effects; D002243:Carbolines / Q000378:metabolism* / Q000494:pharmacology; D002474:Cell-Free System / Q000378:metabolism; D002839:Chromans / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis / Q000378:metabolism; D008550:Melatonin / Q000378:metabolism / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
D000447:Aldehydes; D000975:Antioxidants; D002243:Carbolines; D002839:Chromans; C002272:6-methoxytryptoline; D008315:Malondialdehyde; C009804:tryptoline; D006861:Hydrogen Peroxide; D008550:Melatonin; D001205:Ascorbic Acid; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid