title: Leukocyte and endothelial activation in a laboratory model of extracorporeal membrane oxygenation (ECMO).
abstract: An inflammatory response and a capillary leak syndrome frequently develop during the treatment of neonatal respiratory failure by extracorporeal membrane oxygenation (ECMO). The present study was performed to investigate leukocyte activation and endothelial cell dysfunction that are associated with prolonged contact of blood components with synthetic surfaces. Laboratory ECMO was performed with fresh human blood at 37 degrees C for 8 h (n = 6). Leukocyte activation was measured by L-selectin (CD62L) and CD18 integrin surface expression and by neutrophil-derived elastase release. To monitor endothelial activation, endothelial cell ICAM-1 (CD54) expression was measured in cultured endothelial cells from human umbilical veins (HUVEC) after incubation with plasma from the ECMO experiments. CD18 integrin expression was found significantly up-regulated on polymorphonuclear neutrophils and monocytes after 2-4 h of laboratory ECMO. L-selectin was reduced on both cell types during the total duration of the experiments. Soluble L-selectin (sCD62L) and total and differential leukocyte counts remained unchanged during the experiment. Neutrophil-derived elastase content was maximal after 8 h of ECMO. Plasma from the ECMO experiments did not induce ICAM-1 expression of cultured HUVEC. We conclude that prolonged contact with synthetic surfaces during ECMO activates phagocytes, which may contribute to the inflammatory response seen in ECMO-treated patients. Activated phagocytes do not accumulate in the extracorporeal system nor release humoral factors inducing ICAM-1 expression on endothelial cells.
authors.affiliation: Department of Neonatology, Charité School of Medicine, Campus Virchow, Humboldt Universität, Berlin, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Graulich
authors.forename: B
authors.initials: B
authors.lastname: Walzog
authors.forename: M
authors.initials: M
authors.lastname: Marcinkowski
authors.forename: K
authors.initials: K
authors.lastname: Bauer
authors.forename: H
authors.initials: H
authors.lastname: Kössel
authors.forename: G
authors.initials: G
authors.lastname: Fuhrmann
authors.forename: C
authors.initials: C
authors.lastname: Bührer
authors.forename: P
authors.initials: P
authors.lastname: Gaehtgens
authors.forename: H T
authors.initials: HT
authors.lastname: Versmold
mesh_terms: D018821:CD18 Antigens / Q000378:metabolism; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000276:immunology / Q000503:physiopathology*; D015199:Extracorporeal Membrane Oxygenation / Q000009:adverse effects*; D006801:Humans; D066298:In Vitro Techniques; D007231:Infant, Newborn; D007249:Inflammation / Q000209:etiology; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D019041:L-Selectin / Q000097:blood / Q000378:metabolism; D007958:Leukocyte Count; D019272:Leukocyte Elastase / Q000097:blood / Q000378:metabolism; D007962:Leukocytes / Q000276:immunology / Q000502:physiology*; D008954:Models, Biological
chemical_list: D018821:CD18 Antigens; D018799:Intercellular Adhesion Molecule-1; D019041:L-Selectin; D019272:Leukocyte Elastase