[Molecular cytogenetic characterization of a new wheat line YW443 with resistance to barley yellow dwarf virus].
A new wheat line YW443 with BYDV resistance and good traits was developed from the combination of PP9-1/Shan7859¿Fengkang 8. The wheat line YW443 was identified by BYDV resistance analysis, genomic in situ hybridization (GISH), RFLP and RAPD analysis. The results indicated as follows: (1) YW443 was resistant to GPV and GAV strains of BYDV; (2) YW443 is a homozygous wheat-Thinopyrum intermedium translocation line with a pair of BYDV resistance genes; (3) The 7StL segment of Th. intermedium carring BYDV resistance gene was transferred onto the distal end of the wheat chromosome 7D long arm, the line YW443 was 7DS.7DL-7StL translocation; (4) A RAPD marker OPR19(-900) could detect the DNA of Th. intermedium 7StL in L1 and L1 derivatives including translocation lines YW443, YW642 etc. The markers may be used as a selecting marker for the BYDV resistance breeding program.
Zhang
Z Y
ZY
Institute of Crop Breeding and Cultivation, CAAS, Beijing, China.
Xin
Z Y
ZY
Chen
X
X
Qian
Y T
YT
Lin
Z S
ZS
Xu
H J
HJ
Ma
Y Z
YZ
D017403:In Situ Hybridization; D017793:Luteovirus; D012150:Polymorphism, Restriction Fragment Length; D019105:Random Amplified Polymorphic DNA Technique; D014178:Translocation, Genetic; D014908:Triticum / Q000235:genetics* / Q000821:virology*