title: Assessment of genetic relatedness of vaginal isolates of Candida albicans from different geographical origins.
abstract: PCR fingerprinting with single non-specific primers was used to type vaginal isolates of C. albicans from Portugal, Angola, Madagascar, and two regions of Germany (Berlin and Munich). In addition to analysing isolates that exhibited the normal biotype of C. albicans, the study included atypical strains that failed to assimilate glucosamine and N-acetylglucosamine, which were isolated from women in Angola and Madagascar. A total of 212 strains of C. albicans were studied, representing 87 different multi-locus genotypes. The genotypes of strains from each geographical population were highly similar but not identical. There was one exception: a strain from Portugal grouped with the typical strains from Angola. The typical and especially the atypical populations from Africa displayed less genotype variation than the populations from Europe. The Portuguese samples exhibited the greatest genotypic heterogeneity. Distance analysis (UPGMA) revealed a statistically weak correlation between genotype and geographical origin of the C. albicans isolates.
authors.affiliation: Institut für Mikrobiologie und Hygiene, Universitätsklinikum Charité, Humboldt-Universität Berlin, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Pinto de Andrade
authors.forename: G
authors.initials: G
authors.lastname: Schönian
authors.forename: A
authors.initials: A
authors.lastname: Forche
authors.forename: L
authors.initials: L
authors.lastname: Rosado
authors.forename: I
authors.initials: I
authors.lastname: Costa
authors.forename: M
authors.initials: M
authors.lastname: Müller
authors.forename: W
authors.initials: W
authors.lastname: Presber
authors.forename: T G
authors.initials: TG
authors.lastname: Mitchell
authors.forename: H J
authors.initials: HJ
authors.lastname: Tietz
mesh_terms: D000293:Adolescent; D000328:Adult; D000810:Angola / Q000453:epidemiology; D002176:Candida albicans / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D002181:Candidiasis, Vulvovaginal / Q000453:epidemiology / Q000382:microbiology*; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000032:analysis; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D005858:Germany / Q000453:epidemiology; D006801:Humans; D008270:Madagascar / Q000453:epidemiology; D008875:Middle Aged; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods; D011174:Portugal / Q000453:epidemiology; D014621:Vagina / Q000382:microbiology*
chemical_list: D004271:DNA, Fungal