[Cloning and sequencing of the maize ribosome-inactivating protein gene].
A gene encoding maize Ribosome-inactivating protein was amplified by means of PCR using mRNA of maize leaves as a template, and cloned into pUC19 vector. The amplified DNA sequence has been determined, which consists of 828 bp and encodes 275 amino acid residues. Comparison with previously reported sequence shows 98.4% homologies in nucleotide sequence and 97.4% in amino acid sequence, respectively.
Fan
Q
Q
Institute of Bio-chemistry Engineering, Dalian University of Technology.
Gao
X R
XR
Li
W L
WL
Jin
L J
LJ
An
L J
LJ
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D054790:Ribosome Inactivating Proteins, Type 2; D012270:Ribosomes / Q000187:drug effects*; D003313:Zea mays / Q000737:chemistry*
D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2; C093156:ribosome-inactivating protein 2, maize; D004247:DNA