Determination of enantiomeric excess of alpha-hydroxy-3-phenoxybenzeneacetonitrile and its n-butyl ester by chiral high-performance liquid chromatography.
Determination of enantiomeric excess of alpha-hydroxy-3-phenoxybenzeneacetonitrile, an important intermediate in the production of several pyrethroid insecticides, is usually done after derivatization and gas chromatographic analysis on a beta-cyclodextrin-based column. In this communication we report a direct determination of enantiomeric excess of alpha-hydroxy-3-phenoxybenzeneacetonitrile and its n-butyl ester by chiral HPLC on Chiralcel OJ (Daicel, Japan) in a single run without derivatization.
Fadnavis
N W
NW
Biotransformations Laboratory, Indian Institute of Chemical Technology, Habsiguda, Hyderabad. fadnavisnw@yahoo.com
Babu
R L
RL
Sheelu
G
G
Deshpande
A
A
D000097:Acetonitriles / Q000032:analysis* / Q000737:chemistry; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013237:Stereoisomerism
D000097:Acetonitriles; C419311:hydroxy-3-phenoxybenzeneacetonitrile