title: Nucleoside diphosphate kinase from the hyperthermophilic archaeon Methanococcus jannaschii: overexpression, crystallization and preliminary X-ray crystallographic analysis.
abstract: Nucleoside diphosphate (NDP) kinase is a key enzyme in maintaining cellular pools of all nucleoside triphosphates. NDP kinase from the hyperthermophilic archaebacterium Methanococcus jannaschii has been overexpressed in Escherichia coli and crystallized at 297 K using polyethylene glycol 4000 as precipitant. The crystal is hexagonal, belonging to the space group P6(3), with unit-cell parameters a = b = 72.89, c = 100.87 A. The asymmetric unit contains two subunits of NDP kinase, with a corresponding crystal volume per protein mass (V(M)) of 2.38 A(3) Da(-1) and a solvent content of 48.3%. Native X-ray diffraction data to 2.30 A resolution have been collected using synchrotron X-rays.
authors.affiliation: Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 151-742, South Korea.
authors.forename: K
authors.initials: K
authors.lastname: Min
authors.forename: H K
authors.initials: HK
authors.lastname: Song
authors.forename: C
authors.initials: C
authors.lastname: Chang
authors.forename: J Y
authors.initials: JY
authors.lastname: Lee
authors.forename: S H
authors.initials: SH
authors.lastname: Eom
authors.forename: K K
authors.initials: KK
authors.lastname: Kim
authors.forename: Y G
authors.initials: YG
authors.lastname: Yu
authors.forename: S W
authors.initials: SW
authors.lastname: Suh
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability; D017017:Methanococcus / Q000201:enzymology*; D009701:Nucleoside-Diphosphate Kinase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification
chemical_list: D011994:Recombinant Proteins; D009701:Nucleoside-Diphosphate Kinase