Universal GFP reporter for the study of vascular development.
We report the generation and characterization of transgenic mouse and zebrafish expressing green fluorescent protein (GFP) specifically in vascular endothelial cells in a relatively uniform fashion. These reporter lines exhibit fluorescent vessels in developing embryos and throughout adulthood, allowing visualization of the general vascular patterns with single cell resolution. Furthermore, we show the ability to purify endothelial cells from whole embryos and adult organs by a single step fluorescence activated cell sorting. We expect that these transgenic reporters will be useful tools for imaging vascular morphogenesis, global gene expression profile analysis of endothelial cells, and high throughput screening for vascular mutations.
Motoike
T
T
Department of Internal Medicine and Molecular Biology, The University of Texas Southwestern Medical Center at Dallas, 75390-8573, USA.
Loughna
S
S
Perens
E
E
Roman
B L
BL
Liao
W
W
Chau
T C
TC
Richardson
C D
CD
Kawate
T
T
Kuno
J
J
Weinstein
B M
BM
Stainier
D Y
DY
Sato
T N
TN
D000818:Animals; D030801:Animals, Genetically Modified; D004730:Endothelium, Vascular / Q000254:growth & development / Q000502:physiology*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins; D006321:Heart / Q000196:embryology; D006579:Heterozygote; D006720:Homozygote; D008164:Luminescent Proteins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D051379:Mice; D018345:Mice, Knockout / Q000196:embryology / Q000254:growth & development; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics* / Q000378:metabolism; D042787:Receptor, TIE-2; D013623:Tail / Q000196:embryology; D015027:Zebrafish / Q000196:embryology*
D008164:Luminescent Proteins; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D049452:Green Fluorescent Proteins; D020794:Receptor Protein-Tyrosine Kinases; D042787:Receptor, TIE-2