Establishment of a human malignant fibrous histiocytoma cell line, COMA. Characterization By conventional cytogenetics, comparative genomic hybridization, and multiplex fluorescence In situ hybridization.
The human COMA cell line has been established from a storiform pleomorphic malignant fibrous histiocytoma (MFH). As expected for this tumor type, a very complex karyotype was observed after R-banding analysis. An extensive analysis by 24-color painting, comparative genomic hybridization (CGH), and fluorescence in situ hybridization (FISH) was performed. Twelve complex marker chromosomes recurrently observed were clearly identified; among them, three were systematically present in all analyzed metaphases. Amplifications detected by CGH were refined by FISH with probes specific for various candidate loci. A significant aneuploidy and numerous micronuclei were observed, which could be related to the anomalies of centriole numbers detected in a proportion of cells. Such an analysis, performed on a series of MFH cell lines, would allow the delineation of the genomic alterations specific for the oncogenesis or progression of this complex tumor type or both.
Mairal
A
A
INSERM U509 Laboratoire de Pathologie Moléculaire des Cancers, Paris, France.
Chibon
F
F
Rousselet
A
A
Couturier
J
J
Terrier
P
P
Aurias
A
A
D000368:Aged; D018385:Centrosome; D018244:Chromosomes, Artificial, Yeast; D005260:Female; D018219:Histiocytoma, Benign Fibrous / Q000235:genetics / Q000473:pathology* / Q000648:ultrastructure; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007621:Karyotyping; D009693:Nucleic Acid Hybridization / Q000379:methods*; D012983:Soft Tissue Neoplasms / Q000235:genetics / Q000473:pathology* / Q000648:ultrastructure; D014407:Tumor Cells, Cultured