title: Noncompetitive inhibition of the glycine receptor-mediated current by melatonin in cultured neurons.
abstract: The effect of melatonin on the glycine receptor-mediated response was studied in cultured chick spinal cord neurons using the whole-cell voltage-clamp recording technique. Melatonin rapidly and reversibly inhibited the glycine-induced current in a dose-dependent fashion, with an EC(50) of 934 microM and a maximal inhibition of 100%. Furthermore, melatonin noncompetitively inhibited the glycine response by an agonist-independent mechanism that was distinct from that of an open-channel blocker.
authors.affiliation: Department of Physiology, College of Medicine, National Cheng Kung University, 70101, Tainan, Taiwan. fongsen@mail.ncku.edu.tw
authors.forename: F S
authors.initials: FS
authors.lastname: Wu
authors.forename: Y C
authors.initials: YC
authors.lastname: Yang
authors.forename: J J
authors.initials: JJ
authors.lastname: Tsai
mesh_terms: D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology*; D002478:Cells, Cultured; D002642:Chick Embryo; D005998:Glycine / Q000494:pharmacology*; D008550:Melatonin / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D018009:Receptors, Glycine / Q000187:drug effects* / Q000502:physiology; D013116:Spinal Cord / Q000187:drug effects / Q000502:physiology
chemical_list: D000927:Anticonvulsants; D018009:Receptors, Glycine; D008550:Melatonin; D005998:Glycine