Effect of enterocin CRL35 on Listeria monocytogenes cell membrane.
The antimicrobial peptide Enterocin CRL35, a class II bacteriocin, produces at high concentrations (8 microg ml(-1)) localized holes in the wall and cellular membrane of Listeria monocytogenes, reflected in the efflux of macromolecules such as proteins and other ultraviolet-absorbing materials. At lower concentrations (0.5 microg ml(-1)), neither ultra structural changes nor macromolecules efflux were observed, however potassium and phosphate ions were released, dissipating the proton motive force. As a result the bacteria were killed.
Minahk
C J
CJ
Departamento de Bioquimica de la Nutricion, Instituto Superior de Investigaciones Biológicas, Instituto de Quimica Biologica, San Miguel de Tucuman, Argentina.
Farías
M E
ME
Sesma
F
F
Morero
R D
RD
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001430:Bacteriocins / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects* / Q000648:ultrastructure; D015169:Colony Count, Microbial / Q000379:methods; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D008564:Membrane Potentials / Q000502:physiology; D010455:Peptides
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D010455:Peptides; C121783:enterocin CRL35