title: Clinical evaluation of a novel chromogenic agar dipslide for diagnosis of urinary tract infections.
abstract: The performance of a novel dipslide (DipStreak; Novamed, Israel) consisting of chromogenic agar (Uriselect 3; Sanofi Pasteur, France) and blood agar media was evaluated prospectively and compared to that of conventional urine culture for the diagnosis of urinary tract infection. A total of 1070 clean-catch urine specimens obtained from 251 hospitalized patients and 819 outpatients were processed. The overall performance of the DipStreak was as follows: sensitivity, 95.7%; specificity, 99.2%; agreement, 89.8%; accuracy, 98%; positive predictive value, 98.5%; and negative predictive value, 97.7%. A total of 270 urine specimens were positive by both DipStreak and conventional culture. The chromogenic agar allowed rapid identification of organisms in 211 (78.1%) cultures, while isolates in the other 59 (21.9%) cultures remained unidentified. The results indicate that the DipStreak device coupled with the Uriselect 3 agar represents a convenient and accurate method for inoculation of urine specimens, quantitation of bacteria, diagnosis of significant bacteriuria, and presumptive identification of isolates.
authors.affiliation: Clinical Microbiology Laboratory, Soroka Medical Center, Ben-Gurion University of the Negev, Beer-Sheva, Israel. pavloj@bgumail.bgu.ac.il
authors.forename: P
authors.initials: P
authors.lastname: Yagupsky
authors.forename: M
authors.initials: M
authors.lastname: Rider
authors.forename: N
authors.initials: N
authors.lastname: Peled
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification; D001424:Bacterial Infections / Q000175:diagnosis / Q000382:microbiology; D002175:Candida / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification; D002177:Candidiasis / Q000175:diagnosis / Q000382:microbiology; D002863:Chromogenic Compounds / Q000378:metabolism*; D003470:Culture Media; D006801:Humans; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D014552:Urinary Tract Infections / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D002863:Chromogenic Compounds; D003470:Culture Media; D011933:Reagent Kits, Diagnostic