The apolipoprotein A-IV-360His polymorphism determines the dietary fat clearance in normal subjects.
Apolipoprotein IV (apo A-IV) has been related to fat absorption and to the activation of some of the enzymes involved in lipid metabolism. Several polymorphic sites within the gene locus for apo A-IV have been detected. Previous studies have shown that the A-IV-2 isoform produces a different plasma lipid response after the consumption of diets with different fat and cholesterol content. The present study was designed to evaluate whether the apo A-IV 360His polymorphism could explain, at least in part, the interindividual variability observed during postprandial lipemia. Fifty-one healthy male volunteers (42 homozygous for the apo A-IV 360Gln allele (Gln/Gln) and nine carriers of the A-IV-360His allele), homozygous for the apo E3 allele, were subjected to a vitamin A-fat load test consisting of 1 g of fat/kg body weight and 60000 IU of vitamin A. Blood was drawn at time 0 and every hour for 11 h. Plasma cholesterol (C), triacylglycerol (TG), and C, TG, apo B-100, apo B-48, apo A-IV and retinyl palmitate (RP) were determined in lipoprotein fractions. Data of postprandial lipemia revealed that subjects with the apo A-IV 360His allele had significantly greater postprandial levels in small triacylglycerol rich lipoproteins (TRL)-C (P<0.02), small TRL-TG (P<0.01) and large TRL-TG (P<0.05) than apo A-IV 360Gln/Gln subjects. In conclusion, the modifications observed in postprandial lipoprotein metabolism in subjects with the A-IV 360His allele could be involved in the different low density lipoprotein (LDL)-C responses observed in these subjects following a diet rich in cholesterol and saturated fats.
Ostos
M A
MA
Unidad de Lípidos y Arteriosclerosis, Hospital Universitario 'Reina Sofía', Avda Menéndez Pidal, s/n. 14004, Córdoba, Spain.
Lopez-Miranda
J
J
Marin
C
C
Castro
P
P
Gomez
P
P
Paz
E
E
Jiménez Perepérez
J A
JA
Ordovas
J M
JM
Perez-Jimenez
F
F
D000328:Adult; D001054:Apolipoproteins A / Q000097:blood / Q000235:genetics*; D004041:Dietary Fats / Q000493:pharmacokinetics*; D004224:Diterpenes; D006801:Humans; D008074:Lipoproteins / Q000097:blood / Q000737:chemistry; D008297:Male; D011110:Polymorphism, Genetic / Q000502:physiology*; D019518:Postprandial Period; D012016:Reference Values; D000084562:Retinyl Esters; D014280:Triglycerides / Q000032:analysis / Q000097:blood / Q000737:chemistry; D014801:Vitamin A / Q000031:analogs & derivatives* / Q000097:blood
D001054:Apolipoproteins A; D004041:Dietary Fats; D004224:Diterpenes; D008074:Lipoproteins; D000084562:Retinyl Esters; D014280:Triglycerides; C022205:apolipoprotein A-IV; D014801:Vitamin A; C014794:retinol palmitate