[The effect of immunosuppression on the expression of perforin and granzyme B mRNA in hamster to rat liver transplantation].
OBJECTIVE
To explore the variation on the expression of perforin and granzyme B gene mRNA to judge the effect of immunosuppression in acute rejection of liver transplantation.


METHODS
The expression of perforin and granzyme B gene mRNA was examined by RT-PCR in hamster to rat liver grafts under the immunosuppression of cyclosporine and splenectomy. Histological findings were studied comparatively.


RESULTS
Cyclosporine and splenectomy could obviously suppress the rejection of liver grafts. The survival time of animals was significantly prolonged (37.1 days). The architecture of hepatic lobule was preserved. There was slight round cell infiltration in the portal tracts and no expression of mRNA of perforin and granzyme B genes could been seen in three weeks after transplantation.


CONCLUSIONS
Perforin and granzyme B genes are of value in judging the effect of immunosuppression.
Zhang
S
S
Fuzhou Military General Hospital.
Wu
M
M
Yao
H
H
D000818:Animals; D006224:Cricetinae; D016572:Cyclosporine / Q000494:pharmacology; D005260:Female; D015870:Gene Expression; D006084:Graft Rejection / Q000276:immunology / Q000517:prevention & control*; D053804:Granzymes; D007166:Immunosuppressive Agents / Q000494:pharmacology; D016031:Liver Transplantation; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000235:genetics*; D008647:Mesocricetus; D054353:Perforin; D052899:Pore Forming Cytotoxic Proteins; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D012697:Serine Endopeptidases / Q000096:biosynthesis / Q000235:genetics*; D013156:Splenectomy
D007166:Immunosuppressive Agents; D008562:Membrane Glycoproteins; D052899:Pore Forming Cytotoxic Proteins; D012333:RNA, Messenger; D054353:Perforin; D016572:Cyclosporine; D053804:Granzymes; C509215:Gzmb protein, rat; D012697:Serine Endopeptidases