Differential expression of mRNAs of GDNF family in the striatum following 6-OHDA-induced lesion.
Changes of mRNA levels of GDNF and its recently discovered congeners persephin, neurturin, artemin in the striatum of lesioned side following 6-hydroxydopamine (6-OHDA) lesion in rodents were investigated with semi-quantitative RT-PCR. Two weeks after the lesions were made, mRNA levels of GDNF family members, except for neurturin, were significantly increased in the striatum on the side ipsilateral to the lesion compared with equivalent tissue of sham control. This increase reached a maximal level 5-7 weeks post-lesion. The marked increase of BDNF mRNA expression was also observed in the ipsilateral striatum with similar time course. These findings suggest that 6-OHDA-induced lesions can change gene expression in denervated target tissue, and that mRNA levels of GDNF family members in striatal cells may be modulated by afferent dopaminergic input in a slow-rising and long-lasting fashion.
Zhou
J
J
Key Laboratory of Neurobiology, Institute of Physiology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences and Shanghai Research Center for Life Sciences, PR China.
Yu
Y
Y
Tang
Z
Z
Shen
Y
Y
Xu
L
L
D000818:Animals; D004298:Dopamine / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D051100:Glial Cell Line-Derived Neurotrophic Factor; D017072:Neostriatum / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D009414:Nerve Growth Factors / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009434:Neural Pathways / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology*; D051101:Neurturin; D016627:Oxidopamine / Q000009:adverse effects; D020734:Parkinsonian Disorders / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013378:Substantia Nigra / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology
C490887:Artn protein, rat; C495041:Gdnf protein, rat; D051100:Glial Cell Line-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D051101:Neurturin; C495057:Nrtn protein, rat; D012333:RNA, Messenger; C110758:persephin; D016627:Oxidopamine; D004298:Dopamine