Association of L-type calcium channels with a vacuolar H(+)-ATPase G2 subunit.
The class C L-type calcium (Ca(2+)) channels have been implicated in many important physiological processes. Here, we have identified a mouse vacuolar H(+)-ATPase (V-ATPase) G2 subunit protein that bound to the C-terminal domain of the pore-forming alpha(1C) subunit using a yeast two-hybrid screen. Protein-protein interaction between the V-ATPase G subunit and the alpha(1C) subunit was confirmed using in vitro GST pull-down assays and coimmunoprecipitation from intact cells. Moreover, treatment of cells expressing L-type Ca(2+) channels with a specific inhibitor of the V-ATPase blocked proper targeting of the channels to the plasma membrane.
Gao
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Department of Molecular Pharmacology and Biological Chemistry, Northwestern University Medical School, Chicago, Illinois, 60611, USA. tigao@ucsd.edu
Hosey
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D000818:Animals; D020746:Calcium Channels, L-Type / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D051379:Mice; D011233:Precipitin Tests; D006180:Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D014018:Tissue Distribution; D020798:Two-Hybrid System Techniques; D025262:Vacuolar Proton-Translocating ATPases
D020746:Calcium Channels, L-Type; D012333:RNA, Messenger; D025262:Vacuolar Proton-Translocating ATPases; D006180:Proton-Translocating ATPases