Ty1-copia retrotransposon-like elements in chickpea genome: their identification, distribution and use for diversity analysis.
Ty1-copia retrotransposon-like elements were amplified from Cicer species using primers derived from the conserved region of the reverse transcriptase gene. Two fragments, of size approximately 280bp and approximately 650 bp, were obtained, which on sequencing showed homology for the Ty1-copia reverse transcriptase region. Interestingly, the approximately 650 bp fragment showed two reverse transcriptase regions, one from Ty1-copia and the other from Tto1 element fused together. The copy number was high in the cultivated Cicer arietinum genome compared with the wild Cicer reticulatum. Genetic diversity among the Cicer species was investigated using the conserved primers which grouped the wild species and the cultivated C. arietinum separately.
Sant
V J
VJ
National Chemical Laboratory, Plant Molecular Biology Unit, Division of Biochemical Sciences, 411 008, Pune, India.
Sainani
M N
MN
Sami-Subbu
R
R
Ranjekar
P K
PK
Gupta
V S
VS
D000595:Amino Acid Sequence; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics*; D007887:Fabaceae / Q000235:genetics*; D018628:Gene Dosage; D014644:Genetic Variation; D018745:Genome, Plant; D008969:Molecular Sequence Data; D010802:Phylogeny; D010946:Plants, Medicinal; D018626:Retroelements / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D018744:DNA, Plant; D018626:Retroelements