Studies of mitochondrial porin incorporation parameters and voltage-gated mechanism with different black lipid membranes.
Our work in general aims to clarify the mechanism of what can be considered as a process of the kinetics of porin incorporation into bilayer planar membranes and to identify the parameters involved. In this paper, we report the results of systematically investigating the kinetics of porin incorporation into bilayer membranes made up of phosphatidylinositol or oxidized cholesterol using a simple and low-cost ac method. By means of a mathematical model, we provide evidence that two concurrent processes are present during the kinetics which can be interpreted as positive/negative cooperativity, and we investigate the parameters' dependence on external applied voltages. We observed a phase transition (or similar phenomenon) which seems to take place during the insertion process. The conductance measurement obtained by using data at the steady state conditions, provided indirect indications of two possible gating mechanisms.
Micelli
S
S
Dipartimento Farmaco-Biologico, Facoltà di Farmacia, Università degli Studi di Bari, Italy. micelli@farmbiol.uniba.it
Gallucci
E
E
Picciarelli
V
V
D015640:Ion Channel Gating; D008051:Lipid Bilayers; D008928:Mitochondria / Q000378:metabolism*; D018272:Porins / Q000378:metabolism*
D008051:Lipid Bilayers; D018272:Porins