A broad-host-range expression vector series including a Ptac test plasmid and its application in the expression of the dod gene of Serratia marcescens (coding for ribulose-5-phosphate 3-epimerase) in Pseudomonas stutzeri.
A series of expression vectors for gram-negative bacteria was constructed which combine broad-host-range, inducible expression from the tac promoter and diverse antibiotic resistance determinants. The tac promoter activity and the repression by lacIq can be quantitated with a separate test plasmid in the strain to be studied. The dod gene of Serratia marcescens was expressed in Pseudomonas stutzeri and was shown to code for D-ribulose-5-phosphate 3-epimerase.
Graupner
S
S
Universitaet Oldenburg, AG Genetik, Fachbereich Biologie, Germany.
Wackernagel
W
W
D001426:Bacterial Proteins / Q000378:metabolism; D001483:Base Sequence; D002238:Carbohydrate Epimerases / Q000235:genetics*; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000032:analysis / Q000378:metabolism; D029721:Drosophila Proteins; D015870:Gene Expression; D005798:Genes, Bacterial; D005822:Genetic Vectors; D005827:Genetics, Microbial; D019476:Insect Proteins / Q000235:genetics*; D000072340:NIMA-Interacting Peptidylprolyl Isomerase; D019696:Peptidylprolyl Isomerase; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics*; D012706:Serratia marcescens / Q000201:enzymology / Q000235:genetics*; D014169:Transformation, Bacterial; D001616:beta-Galactosidase / Q000378:metabolism
D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D029721:Drosophila Proteins; D019476:Insect Proteins; D000072340:NIMA-Interacting Peptidylprolyl Isomerase; D001616:beta-Galactosidase; D002238:Carbohydrate Epimerases; C022553:ribulosephosphate 3-epimerase; D019696:Peptidylprolyl Isomerase; C000606849:dod protein, Drosophila