A seminal vesicle autoantigen of mouse is able to suppress sperm capacitation-related events stimulated by serum albumin.
We studied the effect of a mouse seminal vesicle autoantigen (SVA) on BSA-stimulated functions of mouse sperm. Uncapacitated, capacitated, and acrosome-reacted stages of sperm were morphologically scored, and the cellular zinc content was examined cytologically in a modified Tyrode solution at 37 degrees C for 80 min. More than 85% of control cells remained uncapacitated. Addition of 0.3% SVA to the cell incubation did not affect the cell status. Approximately 65% of cells were capacitated in the incubation medium containing 0.3% BSA. Only 30% of the cells became capacitated after incubation with 0.3% BSA and 0.3% SVA together. The decapacitation effect by 0.3% SVA could be subdued by more than 3% BSA in the cell incubation. Whereas BSA did, SVA did not cause removal of Zn(2+) from sperm, but SVA could suppress the BSA effect. The tyrosine phosphorylated proteins in sperm were detected after incubation in a modified HEPES medium containing 0.3% BSA and/or 0.3% SVA at 37 degrees C for 90 min. Whereas BSA enhanced greatly, SVA did not cause phosphorylation of proteins in the range of M:(r) 40 000-120 000. The BSA-stimulated protein tyrosine phosphorylation could be suppressed by SVA in the cell incubation.
Huang
Y H
YH
Institute of Biological Chemistry, Academia Sinica, and Institute of Biochemical Sciences, College of Science, National Taiwan University, Taipei 106, Taiwan.
Chu
S T
ST
Chen
Y H
YH
D000818:Animals; D001324:Autoantigens / Q000494:pharmacology*; D004822:Epididymis / Q000187:drug effects / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000378:metabolism; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism; D012669:Seminal Vesicles / Q000276:immunology*; D012709:Serum Albumin / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D013075:Sperm Capacitation / Q000187:drug effects*; D015032:Zinc / Q000502:physiology
D001324:Autoantigens; D012709:Serum Albumin; D019000:Phosphotyrosine; D017027:Protein Tyrosine Phosphatases; D015032:Zinc