Determination of biotin and folate in infant formula and milk by optical biosensor-based immunoassay.
Biomolecular interaction analysis was evaluated for the automated analysis of biotin- and folate-supplemented infant formulas and milk powders. The technique was configured as a biosensor-based, nonlabeled inhibition immunoassay using monoclonal antibodies raised against analyte-conjugate. Sample extraction conditions were optimized and antibodies were evaluated for cross-reactivity. Performance parameters included a quantitation range of 2-70 ng/mL, recoveries of 86-102%, agreement against assigned reference values for National Institute of Standards and Technology Standard Reference Material 1846, between-laboratory reproducibility relative standard deviation of 9.1% for biotin and 8.1% for folate, respectively, and equivalence against reference microbiological assay methods for both analytes.
Indyk
H E
HE
Anchor Products, Waitoa, New Zealand.
Evans
E A
EA
Bostrom Caselunghe
M C
MC
Persson
B S
BS
Finglas
P M
PM
Woollard
D C
DC
Filonzi
E L
EL
D000818:Animals; D000918:Antibody Specificity; D015374:Biosensing Techniques; D001710:Biotin / Q000032:analysis*; D002138:Calibration; D005492:Folic Acid / Q000032:analysis*; D005516:Food Microbiology; D006801:Humans; D007118:Immunoassay; D007202:Indicators and Reagents; D007225:Infant Food / Q000032:analysis*; D007231:Infant, Newborn; D008892:Milk / Q000737:chemistry*
D007202:Indicators and Reagents; D001710:Biotin; D005492:Folic Acid