title: Inactivation of a MAPK-like protein kinase and activation of a MBP kinase in germinating barley embryos.
abstract: We provide evidence for involvement of two different 45 kDa protein kinases in rehydration and germination of barley embryos. In dry embryos, a myelin basic protein (MBP) phosphorylating kinase was detected, which could be immunoprecipitated with an anti-MAPK (mitogen-activated protein kinase) antibody. Rehydration of the embryo induced a decrease in activity of this 45 kDa MAPK-like protein kinase. In addition, activity of a MBP kinase of the same molecular weight was subsequently found to be induced. This second MBP kinase activity could not be immunoprecipitated with the anti-MAPK antibody and was induced only in germinating embryos, not in dormant embryos.
authors.affiliation: Center for Phytotechnology, Leiden University/ The Netherlands Organization for Applied Scientific Research (UL/TNO), TNO Department of Applied Plant Sciences, Leiden, The Netherlands. testerink@rulbim.leidenuniv.nl
authors.forename: C
authors.initials: C
authors.lastname: Testerink
authors.forename: M
authors.initials: M
authors.lastname: Vennik
authors.forename: J W
authors.initials: JW
authors.lastname: Kijne
authors.forename: M
authors.initials: M
authors.lastname: Wang
authors.forename: S
authors.initials: S
authors.lastname: Heimovaara-Dijkstra
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000737:chemistry / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D004790:Enzyme Induction / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018525:Germination / Q000187:drug effects / Q000502:physiology*; D038362:Glycogen Synthase Kinase 3; D006027:Glycosides / Q000494:pharmacology; D001467:Hordeum / Q000187:drug effects / Q000196:embryology / Q000201:enzymology* / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000737:chemistry / Q000378:metabolism*; D008970:Molecular Weight; D010766:Phosphorylation / Q000187:drug effects; D010937:Plant Growth Regulators / Q000494:pharmacology; D011233:Precipitin Tests; D012639:Seeds / Q000187:drug effects / Q000196:embryology / Q000201:enzymology* / Q000378:metabolism; D014867:Water / Q000378:metabolism / Q000494:pharmacology
chemical_list: D006027:Glycosides; D010937:Plant Growth Regulators; D014867:Water; C007808:fusicoccin; D000040:Abscisic Acid; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D020928:Mitogen-Activated Protein Kinases; D038362:Glycogen Synthase Kinase 3