title: Conservation in probiotic preparations of Lactobacillus with inhibitory capacity on other species.
abstract: Strains of Lactobacillus isolated from dairy products and genital tract competed with Candida albicans through a membrane of 12000 dalton cut-off. This inhibition was due to hydrogen peroxide and was trypsin-stable, heat-sensitive and antagonized by catalase. Lactobacillus coming from "starters" showed antimicrobial activity against fungus isolated in a yogurt factory. Penicillium, Alternaria, Phialophora, Microsporum and Candida spp. were inhibited when 10(2) spores were inoculated in the assay. No inhibition was observed with 10(5) spores. Besides, one of 21 Lactobacillus strains isolated from the vaginas of healthy women inhibited pathogenic bacteria by means a bacteriocin trypsin-sensitive, heat-stable and retained by dialysis membrane. Tablets for future probiotic use were prepared and the viability of bacteria was assayed using media with different compositions. Pharmaceutical preparations with polyethyleneglycol was the best formulation for the Lactobacillus viability, the counts remained between 10(7) and 10(6) cfu/tablet for up to 1 year.
authors.affiliation: Departamento de Farmacia-Facultad de Ciencias Químicas-Universidad Nacional de Córdoba.
authors.forename: M G
authors.initials: MG
authors.lastname: Paraje
authors.forename: I
authors.initials: I
authors.lastname: Albesa
authors.forename: A J
authors.initials: AJ
authors.lastname: Eraso
mesh_terms: D000898:Antibiosis; D001430:Bacteriocins / Q000494:pharmacology; D002175:Candida / Q000187:drug effects; D003611:Dairy Products / Q000382:microbiology; D004339:Drug Compounding / Q000379:methods; D004355:Drug Stability; D005260:Female; D006194:Gardnerella vaginalis / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007778:Lactobacillus; D009344:Neisseria gonorrhoeae / Q000187:drug effects; D019936:Probiotics / Q000494:pharmacology*; D014621:Vagina / Q000382:microbiology
chemical_list: D001430:Bacteriocins; C035728:lactocin; D006861:Hydrogen Peroxide