Changes in cardiac energy metabolism during early development of female SHR.
We investigated effects of hypertension and early development on myocardial energy metabolism as reflected by maximal enzyme activities, glucose transporter content, and endogenous substrates in female Wistar-Kyoto (WKY) rats and spontaneously hypertensive rats (SHR). Left ventricular hypertrophy and systolic hypertension were evident in SHR at 6 weeks of age and these differences increased at 14 and 22 weeks of age. 3-Hydroxyacyl-CoA dehydrogenase (HOAD) activity in the left ventricle was 18% lower in 6-week-old rats than both 14- and 22-week-old rats, but not different between WKY rats and SHR. Hexokinase activity was 15% lower in 6-week-old SHR than WKY rats and decreased progressively with age in both strains. Glucose transporter (GLUT) 1 content was nearly twofold greater in 6-week-old rats than both 14- and 22-week-old rats. We found no difference in citrate synthase activity or GLUT4 content among groups. Glycogen concentration was 44% lower in SHR than WKY rats, whereas triglyceride was slightly (16%) higher in SHR than WKY rats. Older animals had higher levels both glycogen and triglyceride than younger animals. We conclude that the left ventricle of both SHR and WKY rats may change from predominantly glucose to fatty acid oxidation for energy production during early development.
LaPier
T L
TL
Department of Physical and Occupational Therapy, Idaho State University, Pocatello 83209-8007, USA. lapitany@isu.edu
Rodnick
K J
KJ
D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000378:metabolism; D000375:Aging / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development / Q000378:metabolism; D001794:Blood Pressure; D001835:Body Weight; D004734:Energy Metabolism; D005260:Female; D051272:Glucose Transporter Type 1; D051275:Glucose Transporter Type 4; D006593:Hexokinase / Q000378:metabolism; D006973:Hypertension / Q000378:metabolism* / Q000473:pathology; D009004:Monosaccharide Transport Proteins / Q000378:metabolism; D009124:Muscle Proteins; D009206:Myocardium / Q000378:metabolism*; D010210:Papillary Muscles / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR / Q000254:growth & development / Q000378:metabolism*; D011921:Rats, Inbred WKY; D014280:Triglycerides / Q000378:metabolism
D051272:Glucose Transporter Type 1; D051275:Glucose Transporter Type 4; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins; C495679:Slc2a1 protein, rat; C495694:Slc2a4 protein, rat; D014280:Triglycerides; D015094:3-Hydroxyacyl CoA Dehydrogenases; D006593:Hexokinase