title: Quantitative gene expression analysis in microdissected archival tissue by real-time RT-PCR.
abstract: Laser-assisted microdissection is a powerful tool for the analysis of morphologically defined cell populations. We report here that the combination of microdissection and real-time RT-PCR technologies together with an optimized RNA microscale extraction procedure allows the reproducible determination of gene expression levels in archival formalin-fixed, paraffin-embedded tissue samples.
authors.affiliation: GSF-Forschungszentrum für Umwelt und Gesundheit, Institut für Pathologie, Neuherberg, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Specht
authors.forename: T
authors.initials: T
authors.lastname: Richter
authors.forename: U
authors.initials: U
authors.lastname: Müller
authors.forename: A
authors.initials: A
authors.lastname: Walch
authors.forename: M W
authors.initials: MW
authors.lastname: Höfler
mesh_terms: D004210:Dissection / Q000379:methods*; D015870:Gene Expression; D006652:Histological Techniques; D006801:Humans; D007834:Lasers; D009369:Neoplasms / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012313:RNA; D018719:Erb-b2 Receptor Tyrosine Kinases