On the modulation of photoinduced fluorescence enhancement and conformational stability of albumin-bound bilirubin: effect of epsilon-NH(2) groups blocking and chloroform binding.
Photoinduced fluorescence enhancement of bilirubin bound to primary binding site on human serum albumin (HSA) was completely ceased when epsilon-NH(2) groups of its internal lysine residues were covalently blocked by acetylation or succinylation though the pigment bound to these derivatives in a folded conformation akin to that bound to HSA. These photoinduced fluorescence modulations cannot be ascribed to the binding of bilirubin to secondary low affinity sites as the CD spectrum of bilirubin bound to these derivatives showed complete inversion upon addition of chloroform which binds to subdomain IIA in HSA where high affinity bilirubin binding site is located. Presence of chloroform reconciled the photoinduced alterations in the CD spectrum observed in its absence, suggesting that chloroform stabilized the bound ligand against light but the fluorescence properties of bilirubin complexed with acetylated or succinylated derivatives remained unchanged. Guanidination of internal epsilon-NH(2) groups in HSA by O-methylisourea did not alter the spectral properties of the bound ligand. These results suggest that salt linkage(s) existing between epsilon-NH(2) groups of lysine residues in HSA and carboxyl groups of bilirubin, act(s) as a potential barrier during conformational rotation of the bound ligand assisted by photoactivation and their abolishment can alter its dynamics and stereoselectivity, a hitherto unnoticed implication of salt linkage(s) in BR-HSA complex.
Khan
M M
MM
Interdisciplinary Biotechnology Unit, Aligarh Muslim University, 202002, Aligarh, India.
Tayyab
S
S
D001663:Bilirubin / Q000737:chemistry / Q000378:metabolism*; D002725:Chloroform / Q000493:pharmacokinetics*; D002942:Circular Dichroism; D006801:Humans; D007700:Kinetics; D008027:Light; D008239:Lysine; D011487:Protein Conformation; D012709:Serum Albumin / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
D012709:Serum Albumin; D002725:Chloroform; D008239:Lysine; D001663:Bilirubin