title: 5 S ribosomal spacer sequences of some filarial parasites: comparative analysis and diagnostic applications.
abstract: We have amplified by PCR the sequences of the 5 S ribosomal spacer of Setaria labiatopapillosa and Foleyella furcata. After sequencing, these sequences have been compared with those of Dirofilaria immitis and Dirofilaria repens. Two major goals have been achieved: (i) the establishment of a multiplex PCR-based diagnostic assay, applicable to identify the four species in vertebrate and invertebrate hosts; (ii) the identification, in S. labiatopapillosa and F. furcata, of a canonical spliced leader 1 (SL1) sequence, so confirming that only D. repens, of the filarial parasites so far studied, shows a peculiar SL1 sequence. The PCR assay here developed and the analysis of the 5 S ribosomal spacer, can further improve both epidemiological and molecular analysis of these filarial species.
authors.affiliation: Istituto di Patologia Generale Veterinaria dell'Università degli Studi di Milano, via Celoria 10, Milano, 20133, Italy. favia@axrma.uniroma1.it
authors.forename: G
authors.initials: G
authors.lastname: Favia
authors.forename: G
authors.initials: G
authors.lastname: Cancrini
authors.forename: I
authors.initials: I
authors.lastname: Ricci
authors.forename: C
authors.initials: C
authors.lastname: Bazzocchi
authors.forename: M
authors.initials: M
authors.lastname: Magi
authors.forename: M
authors.initials: M
authors.lastname: Pietrobelli
authors.forename: C
authors.initials: C
authors.lastname: Genchi
authors.forename: C
authors.initials: C
authors.lastname: Bandi
mesh_terms: D000818:Animals; D001483:Base Sequence; D021903:DNA, Ribosomal Spacer; D004182:Dirofilaria / Q000235:genetics* / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012689:Sequence Homology, Nucleic Acid; D017182:Setaria Nematode / Q000235:genetics / Q000302:isolation & purification; D013045:Species Specificity
chemical_list: D021903:DNA, Ribosomal Spacer