Effects of the Na(+)/H(+) exchanger monensin on intracellular pH in astroglia.
Stimulation of astroglial glucose utilization by the Na(+)/H(+) exchanger monensin is only partially blocked by ouabain. The present studies show that monensin also raises intracellular pH in astroglia. Because increased pH stimulates phosphofructokinase activity, the ouabain-insensitive portion of the stimulation of cerebral glucose utilization (CMR(glc)) appears to be due to stimulation of glycolysis by intracellular alkalinization.
Itoh
Y
Y
Laboratory of Cerebral Metabolism, National Institute of Mental Health, Bethesda, MD 20892-4030, USA.
Law
M J
MJ
Sokoloff
L
L
D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000187:drug effects / Q000502:physiology; D005260:Female; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D007476:Ionophores / Q000494:pharmacology*; D008985:Monensin / Q000494:pharmacology*; D010732:Phosphofructokinase-1 / Q000187:drug effects / Q000502:physiology; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D017923:Sodium-Hydrogen Exchangers / Q000187:drug effects* / Q000502:physiology
D007476:Ionophores; D017923:Sodium-Hydrogen Exchangers; D008985:Monensin; D010732:Phosphofructokinase-1