Development of a fetal challenge method for the evaluation of bovine viral diarrhea virus vaccines.
A method to evaluate the efficacy of bovine viral diarrhea virus (BVDV) vaccines using a multiple challenge model was investigated. Four pregnant heifers were challenged intranasally with a type I and type II isolate of BVDV at 75 days of gestation. At 60 days postinoculation, virus isolation and RT-PCR from blood and tissues of fetuses indicated that all fetus were persistently infected with both type I and type II isolates. Differing results of detection by PCR and virus isolation between the type I and type II isolates were obtained. These preliminary studies may indicate differences in the level of replication between type I and type II BVDV as well as predilected sites of replication in certain tissues.
Brock
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KV
Department of Pathobiology, College of Veterinary Medicine, 264 Greene Hall, Auburn University, Auburn, AL 36849-5519, USA. brockv@vetmed.auburn.edu
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D000818:Animals; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000517:prevention & control*; D002417:Cattle; D002478:Cells, Cultured; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology*; D005260:Female; D005315:Fetal Diseases / Q000517:prevention & control*; D018445:Infectious Disease Transmission, Vertical / Q000517:prevention & control / Q000662:veterinary*; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D014611:Vaccination / Q000662:veterinary*; D014765:Viral Vaccines / Q000276:immunology*
D014765:Viral Vaccines