title: Regulation of rat magnocellular neurosecretory system by D-aspartate: evidence for biological role(s) of a naturally occurring free D-amino acid in mammals.
abstract: Little evidence is available for the physiological function of D-amino acids in species other than bacteria. Here we demonstrate that naturally occurring freed -aspartate (D-Asp) is present in all magnocellular neurons of rat hypothalamus. The levels of this naturally occurring D-amino acid were elevated during lactation and returned to normal thereafter in the magnocellular neurosecretory system, which produces oxytocin, a hormone responsible for milk ejection during lactation. Intraperitoneal injections of D-Asp reproducibly increased oxytocin gene expression and decreased the concentration of circulating oxytocin in vivo. Similar changes were observed in the vasopressin system. These results provide evidence for the role(s) of naturally occurring free D-Asp in mammalian physiology. The findings argue against the conventional concept that only L-stereoisomers of amino acids are functional in higher species.
authors.affiliation: Department of Neurology, Harvard Medical School and Center for Neurologic Diseases, Brigham and Women's Hospital, 77 Avenue Louis Pasteur, Boston, Massachusetts 02115, USA. h.wang@uke.uni-hamburg.de
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.forename: H
authors.initials: H
authors.lastname: Wolosker
authors.forename: J
authors.initials: J
authors.lastname: Pevsner
authors.forename: S H
authors.initials: SH
authors.lastname: Snyder
authors.forename: D J
authors.initials: DJ
authors.lastname: Selkoe
mesh_terms: D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D007031:Hypothalamus / Q000502:physiology*; D007774:Lactation / Q000502:physiology; D009476:Neurons, Efferent / Q000378:metabolism; D009490:Neurosecretory Systems / Q000502:physiology*; D010121:Oxytocin / Q000097:blood / Q000235:genetics; D051381:Rats; D020318:Rats, Long-Evans
chemical_list: D001224:Aspartic Acid; D010121:Oxytocin