title: [Complete nucleotide sequences and genome structure of two Chinese tobacco mosaic virus isolates deduced from full-length infectious cDNA clones].
abstract: The complete nucleotides of two Chinese tobacco mosaic virus (TMV) isolates, TMV-Cv (vulgare strain) and TMV-N14 (an attenuated virus originated from a tomato strain), were determined from their respective full-length infectious cDNA clones and compared with published TMV sequences. The genome structure of TMV-Cv contained 6395 nucleotides, in which four functional open reading frames (ORF), coding for replicase (126 kD/183 kD), movement protein (MP, 30 kD) and coat protein (CP, 17.6 kD) respectively, could be recognized. TMV-N14 contained 6384 nucleotides in its genome. In contrast to TMV-Cv, five functional ORFs encoding the replicase 98.5 kD/126 kD/183 kD, MP(27 kD) and CP(17.6 kD), respectively, were detected in the TMV-N14 genome. TMV-Cv is 99% homologous to a Korean TMV isolate belonging to the vulgare strain at the nucleotide level. TMV-N14 is 99% homologous to a highly virulent Japanese isolate TMV-L (tomato strain) at the nucleotide level. In TMV-N14, one opal nulation (UGA) occurred in the replicase gene and one ochre nutation (UAA) in the MP gene. The former mutation created a potential, additional ORF within the replicase gene, the latter reduced the size of the MP to 27 kD. In addition, there were also 13 amino acid substitutions in the replicase gene of TMV-N14 when compared to that of TMV-L. Collectively, these changes may have significant implications in the attenuation of the virulence of TMV-N14.
authors.affiliation: Department of Molecular Virology and Bioengineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing.
authors.forename: G
authors.initials: G
authors.lastname: Yang
authors.forename: X G
authors.initials: XG
authors.lastname: Liu
authors.forename: B S
authors.initials: BS
authors.lastname: Qiu
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry*; D004279:DNA, Viral / Q000737:chemistry*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D014027:Tobacco Mosaic Virus / Q000235:genetics*; D014613:Vaccines, Attenuated
chemical_list: D018076:DNA, Complementary; D004279:DNA, Viral; D014613:Vaccines, Attenuated; D012324:RNA-Dependent RNA Polymerase