Retrovirus vector containing wild type p53 gene and its effect on human glioma cells.
A retroviral vector containing wild-type p53 tumor suppressor gene (wt-p53) under the control of viral LTR sequences was constructed and transfected into packaging cell line GP+envAm12. Virus producing single cell clone GP+envAm12/ p53clC8 (8 x 10(5) cfu/ml, determined on NIH 3T3 cells) was isolated and used to transfer wt-p53 gene into human glioma cell lines in vitro. Decreased viability in p53-infected cells as compared to uninfected or empty virus infected cells was observed.
Hlavatý
J
J
Cancer Research Institute, Slovak Academy of Sciences, Bratislava.
Tyukosova
S
S
Bies
J
J
Hlubinova
K
K
Altaner
C
C
D016475:3T3 Cells / Q000821:virology; D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology; D002470:Cell Survival / Q000235:genetics; D002945:Cisplatin / Q000008:administration & dosage; D004317:Doxorubicin / Q000008:administration & dosage; D005047:Etoposide / Q000008:administration & dosage; D016158:Genes, p53 / Q000235:genetics*; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000235:genetics / Q000494:pharmacology*; D005910:Glioma / Q000188:drug therapy / Q000235:genetics / Q000628:therapy*; D006801:Humans; D051379:Mice; D012190:Retroviridae / Q000235:genetics*; D014162:Transfection / Q000379:methods*; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology
D016159:Tumor Suppressor Protein p53; D005047:Etoposide; D004317:Doxorubicin; D002945:Cisplatin