[Comparative effectiveness of antioxidants in protecting the bacterial plasmatic membrane from the active froms of oxygen].
The effect of hydroxyl radicals OH. generated by the decomposition of H2O2 by Fe2+ ions (Fenton reaction) on the barrier properties of plasma membranes of Escherichia coli cells K-12 was studied by electroorientation spectroscopy. It was found that the administration of hydrogen peroxide led to the disturbance of the barrier properties of plasma membranes only when the cells were preincubated with Fe2+ ions and their constant concentration in the system was maintained by ascorbate or dithiotreitol (150-500 microM). The extent of the toxic action on plasma membranes depended on the concentration of reacting elements and the substance used as a reducer Fe2+. The efficiency of protection of antioxidants of different classes (enzymic, SH-containing, and phenolic compounds) against the toxic action of hydroxyl radicals on plasmatic membranes was shown.
Ivanov
A Iu
AIu
Institute of Cell Biophysics, Ruasian Academy of Sciences, Pushchino, Moscow Region, Russia.
Novoselov
V I
VI
Gavriushkin
A V
AV
Fesenko
E E
EE
D000975:Antioxidants / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D017382:Reactive Oxygen Species; D013057:Spectrum Analysis / Q000379:methods
D000975:Antioxidants; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide