Producing a low ovomucoid egg white preparation by precipitation with aqueous ethanol.
A novel method for producing a low ovomucoid egg white preparation is proposed. Egg white powder (0.5 g) was dissolved in a 10-fold weight of distilled water and adjusted to pH 5, and ethanol was added to the solution at a final concentration of 20% (v/v). The mixture was vigorously stirred and centrifuged. The precipitate was washed three times with 20% ethanol (6.25 ml each), with about 65% of egg white proteins occurring in the precipitate. The use of ELISA demonstrated that 70% of ovomucoid was recovered from the supernatant fraction. However, functionally important proteins such as ovalbumin, ovotransferrin, and lysozyme still remained in the precipitate. These results may be due primarily to the much higher solubility of ovomucoid in this aqueous ethanol. Food quality evaluation showed that high whippability and foam stability were retained in the low ovomucoid preparation as in its material egg white. This product would thus be applicable as a new processed food for ovomucoid-sensitive allergic patients.
Tanabe
S
S
Food Science Laboratory, Faculty of Education, Tokyo Gakugei University, Japan. stana@u-gakugei.ac.jp
Tesaki
S
S
Watanabe
M
M
D000818:Animals; D011232:Chemical Precipitation; D002645:Chickens; D003207:Conalbumin / Q000032:analysis; D004529:Egg White; D004797:Enzyme-Linked Immunosorbent Assay; D000431:Ethanol; D005511:Food Handling; D009113:Muramidase / Q000032:analysis; D010047:Ovalbumin / Q000032:analysis; D010059:Ovomucin / Q000302:isolation & purification*
D003207:Conalbumin; D010059:Ovomucin; D000431:Ethanol; D010047:Ovalbumin; D009113:Muramidase