Different cell death mechanisms and gene expression in human cells induced by pentachlorophenol and its major metabolite, tetrachlorohydroquinone.
Pentachlorophenol (PCP) and its salt are used extensively as biocide and wood preservative. Due to improper disposal, PCP has become an environmental pollutant and is now considered to be ubiquitos. Metabolic studies carried out in rodents or human liver homogenate have indicated that PCP undergoes oxidative dechlorination to form tetrachlorohydroquinone (TCHQ). The cytotoxicity, cell death mechanisms and gene expression of PCP and TCHQ are investigated in human liver and bladder cells and show that TCHQ induces apoptosis and DNA genomic fragmentation in bladder cells but not liver cells. No apoptotic features could be induced by treatment of PCP in both cell lines. The concentrations of PCP required to cause 50% cell death in T-24 and Chang liver cells were 5-10-fold greater than the concentrations of TCHQ. Several gene products are important in controlling the apoptotic and necrotic processes. Of these, hsp 70, CAS, bcl-2 and bax were studied. The expression of the hsp70 gene increased significantly (2-3-fold) in cells treated with TCHQ. However, no significant change was found in the cells treated with PCP. The expression of CAS gene decreased significantly in T-24 cells treated with both TCHQ and PCP. Whereas, no significant change was found in Chang liver cells with the same treatment. In addition, the expression of the bcl-2/bax protein decreased significantly in these two cell lines treated with TCHQ but not PCP.
Wang
Y J
YJ
Department of Environmental and Occupational Health, National Cheng Kung University, Medical College, 138 Sheng-Li Road, 70428, Tainan, Taiwan, ROC.
Ho
Y S
YS
Jeng
J H
JH
Su
H J
HJ
Lee
C C
CC
D017209:Apoptosis / Q000187:drug effects; D002273:Carcinogens / Q000494:pharmacology*; D016923:Cell Death / Q000235:genetics*; D002460:Cell Line; D029563:Cellular Apoptosis Susceptibility Protein; D053938:DNA Fragmentation; D004785:Environmental Pollutants; D005434:Flow Cytometry; D015870:Gene Expression / Q000187:drug effects*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D006873:Hydroquinones / Q000494:pharmacology*; D020287:In Situ Nick-End Labeling; D008099:Liver / Q000166:cytology / Q000187:drug effects; D010416:Pentachlorophenol / Q000494:pharmacology*; D011506:Proteins / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D014407:Tumor Cells, Cultured; D001743:Urinary Bladder / Q000166:cytology / Q000187:drug effects; D051028:bcl-2-Associated X Protein
C494826:BAX protein, human; D002273:Carcinogens; D029563:Cellular Apoptosis Susceptibility Protein; D004785:Environmental Pollutants; D018840:HSP70 Heat-Shock Proteins; D006873:Hydroquinones; D011506:Proteins; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; C044578:2,3,5,6-tetrachlorohydroquinone; D010416:Pentachlorophenol