Characterisation of a Pasteurella multocida esterase gene which confers a hemolytic phenotype in Escherichia coli under anaerobic conditions.
Investigation of the hemolytic phenotype under anaerobic growth conditions of an avian Pasteurella multocida strain, PBA100, resulted in the identification and characterisation of a gene encoding an esterase enzyme, mesA, that conferred a hemolytic phenotype in Escherichia coli under anaerobic conditions. MesA appeared to be expressed and functional under anaerobic and aerobic conditions in both E. coli and P. multocida. A P. multocida mesA mutant was generated which resulted in the loss of acetyl esterase activity under anaerobic conditions. However, this mutation did not cause any attenuation of virulence for mice nor a detectable change to the anaerobic hemolytic phenotype of P. multocida. In E. coli MesA appeared to cause hemolysis indirectly by the induction of the latent E. coli K-12 cytolysin, sheA.
Hunt
M L
ML
Bacterial Pathogenesis Research Group, Department of Microbiology, Monash University, 3800, Clayton, Vic., Australia.
Cox
A J
AJ
Ruffolo
C G
CG
Rajakumar
K
K
Adler
B
B
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002417:Cattle; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D004950:Esterases; D005798:Genes, Bacterial; D006461:Hemolysis / Q000235:genetics; D006736:Horses; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009154:Mutation; D016979:Pasteurella multocida / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity; D010641:Phenotype; D016415:Sequence Alignment; D012756:Sheep; D013552:Swine; D014774:Virulence
D001426:Bacterial Proteins; D004950:Esterases; C417605:MesA protein, Pasteurella multocida