Expression, crystallization and preliminary X-ray diffraction studies of recombinant Bacillus anthracis lethal factor.
The lethal factor (LF) produced by Bacillus anthracis is a Zn(2+)-dependent endopeptidase which specifically cleaves the N-terminal tail of several MAP kinase kinases (MAPKKs). The recombinant expression, purification and crystallization of LF and of an inactive mutant consisting of a single amino-acid substitution in the conserved catalytic site are reported here. Both proteins crystallize in the cubic space group I432.
Bernardi
L
L
Centro CNR Biomembrane and Dipartimento di Scienze Biomediche, Università di Padova, 35121 Padova, Italy.
Vitale
G
G
Montecucco
C
C
Musacchio
A
A
D001408:Bacillus anthracis / Q000737:chemistry*; D001427:Bacterial Toxins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry
D001427:Bacterial Toxins; D011994:Recombinant Proteins