title: Expression of interleukin-6 receptor, leukemia inhibitory factor receptor and glycoprotein 130 in the murine cerebellum and neuropathological effect of leukemia inhibitory factor on cerebellar Purkinje cells.
abstract: Expression of glycoprotein 130 and the related receptors, including interleukin-6 receptor and leukemia inhibitory factor receptor, was examined in the murine cerebellum at the protein level. Western blot analysis revealed that interleukin-6 receptor, leukemia inhibitory factor receptor and glycoprotein 130 were expressed in the murine cerebellum. Immunoreactivities for interleukin-6 receptor, leukemia inhibitory factor receptor and glycoprotein 130 were strongly localized on the cell body of Purkinje cells, indicating that both interleukin-6 and leukemia inhibitory factor could act directly on Purkinje cells in murine adult mice. The expressions of interleukin-6 receptor, leukemia inhibitory factor receptor and glycoprotein 130 were observed on the cell membranes of Purkinje cells by immunoelectron microscopy. Immunoreactivity for the interleukin-6 receptor was also detected in the cytoplasm of Purkinje cells. Injection of a murine hemopoietic cell line, FDC-P1 cells, transfected with the complementary DNA encoding the leukemia inhibitory factor led to a reduction in calbindin-positive dendrites of the Purkinje cells.The present results suggest that the leukemia inhibitory factor affects cerebellar functions through Purkinje cells.
authors.affiliation: Department of Anatomy and Neurobiology, Wakayama Medical School, 811-1, Kimiidera, Wakayama City, 641-0012, Wakayama, Japan. yoshim@wakayama-med.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Morikawa
authors.forename: K
authors.initials: K
authors.lastname: Tohya
authors.forename: S
authors.initials: S
authors.lastname: Tamura
authors.forename: M
authors.initials: M
authors.lastname: Ichihara
authors.forename: A
authors.initials: A
authors.lastname: Miyajima
authors.forename: E
authors.initials: E
authors.lastname: Senba
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D017690:Cell Transplantation; D002531:Cerebellum / Q000378:metabolism* / Q000473:pathology; D003712:Dendrites / Q000473:pathology; D006131:Growth Inhibitors / Q000378:metabolism*; D007150:Immunohistochemistry; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; D008222:Lymphokines / Q000378:metabolism*; D051907:Lysosomal Membrane Proteins; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D016253:Microscopy, Immunoelectron; D011689:Purkinje Cells / Q000473:pathology* / Q000648:ultrastructure; D018121:Receptors, Cytokine / Q000378:metabolism*; D019947:Receptors, Interleukin-6 / Q000378:metabolism*; D053606:Receptors, OSM-LIF; D052118:Lysosomal-Associated Membrane Protein 1
chemical_list: D006131:Growth Inhibitors; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; D008222:Lymphokines; D051907:Lysosomal Membrane Proteins; D008562:Membrane Glycoproteins; D018121:Receptors, Cytokine; D019947:Receptors, Interleukin-6; D053606:Receptors, OSM-LIF; C052255:Lamp1 protein, mouse; D052118:Lysosomal-Associated Membrane Protein 1; C507838:Lif protein, mouse; C507841:Lifr protein, mouse