Increased potassium concentration inhibits stimulation of vascular smooth muscle proliferation by PDGF-BB and bFGF.
The effects of changes in extracellular potassium concentration on the rate of vascular smooth muscle cell proliferation stimulated by cytokines and serum were analyzed in vitro. To analyze the DNA synthesis response, cells from swine coronary artery were grown in DMEM medium containing 3, 4, 5, or 6 mmol/L potassium together with 20 ng/mL platelet-derived growth factor BB (PDGF-BB), 25 ng/mL basic fibroblast growth factor (bFGF), or 5% fetal bovine serum (FBS), with [methyl 3H] thymidine added, for 24 h. Proliferation responses were analyzed in cells grown in medium with potassium concentrations of 3, 4, 5, or 6 mmol/L, together with either 20 ng/mL PDGF-BB, 25 ng/mL bFGF, or 5% FBS, for 7 days, then harvested and counted. Highly significant inverse relationships were observed between potassium concentration and 3H-thymidine incorporation stimulated by each of the three agonists (P < .01 for each, ANOVA), and between potassium concentration and proliferation (all P < .01, ANOVA). Elevation of potassium concentration within the physiologic range inhibits vascular smooth muscle cell DNA synthesis and proliferation.
Ma
G
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Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson 39216-4505, USA. mag@gwgate.nhlbi.nih.gov
Mamaril
J L
JL
Young
D B
DB
D000818:Animals; D000077214:Becaplermin; D002417:Cattle / Q000097:blood / Q000196:embryology; D002455:Cell Division / Q000187:drug effects; D004247:DNA / Q000037:antagonists & inhibitors; D005312:Fetal Blood / Q000502:physiology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000378:metabolism; D009994:Osmolar Concentration; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D011188:Potassium / Q000494:pharmacology*; D020574:Proto-Oncogene Proteins c-sis; D013552:Swine; D013936:Thymidine / Q000378:metabolism
D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D016222:Fibroblast Growth Factor 2; D000077214:Becaplermin; D004247:DNA; D011188:Potassium; D013936:Thymidine