Detection of Legionella pneumophila using a real-time PCR hybridization assay.
A real-time PCR hybridization assay for Legionella pneumophila is described; the assay uses LightCycler (Idaho Technology) methodology to specifically detect 2.5 CFU/reaction, equivalent to 1,000 CFU/liter of starting water sample. The assay, including DNA extraction and confirmation of product identity, is completed within 90 min of receipt of a sample.
Ballard
A L
AL
Department of Microbiology and PHLS Laboratory, University Hospital, Nottingham NG7 2UH, United Kingdom.
Fry
N K
NK
Chan
L
L
Surman
S B
SB
Lee
J V
JV
Harrison
T G
TG
Towner
K J
KJ
D004269:DNA, Bacterial / Q000032:analysis; D016952:Legionella pneumophila / Q000235:genetics / Q000302:isolation & purification*; D007877:Legionnaires' Disease / Q000382:microbiology / Q000517:prevention & control* / Q000635:transmission; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity; D014871:Water Microbiology
D004269:DNA, Bacterial