Integrin expression is upregulated during early healing in a canine intrasynovial flexor tendon repair and controlled passive motion model.
To explore crucial early molecular events involved in contact healing of the intrasynovial flexor tendon, integrin expression was evaluated at the transcriptional and post-transcriptional levels during the first two weeks following injury, repair and controlled passive motion in a canine model. Specifically, immunohistochemical and reverse transcription polymerase chain reaction (RT-PCR) techniques were employed to evaluate expression of the fibronectin, vitronectin and endothelial cell binding integrin receptor subunits alpha5, alphav and alpha6, along with the common beta1 subunit. The two techniques revealed increasing expression of the four subunits over the two week post-repair period. Immunohistochemistry revealed that beta1 and alpha5 expression was concentrated in the epitenon layer near the repair site and interiorly within the wound area, while alpha6 was associated with capillary-forming endothelial cells near the wound. RT-PCR and quantitation by NIH image analysis demonstrated peak messenger RNA expression of beta1 and alpha5 at ten days post-repair and peak expression of alpha6 and alphav at 15 days. The results in this study correlate well with previous results demonstrating increased fibronectin deposition and angiogenesis during the same time period in a similar injury/repair model.
Harwood
F L
FL
Department of Orthopaedics, Connective Tissue Biochemistry, University of California San Diego, La Jolla 92093-0630, USA.
Monosov
A Z
AZ
Goomer
R S
RS
Gelberman
R H
RH
Winters
S C
SC
Silva
M J
MJ
Amiel
D
D
D000818:Animals; D004285:Dogs; D007091:Image Processing, Computer-Assisted; D007103:Immobilization; D007124:Immunoenzyme Techniques; D016023:Integrins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D023421:Models, Animal; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013583:Synovial Membrane / Q000502:physiology; D013710:Tendons / Q000378:metabolism / Q000502:physiology*; D015854:Up-Regulation; D014945:Wound Healing
D016023:Integrins; D012333:RNA, Messenger