Evaporative microdialysis: an effective improvement in an established method of protein crystallization.
Evaporative dialysis is a simple variant of conventional microdialysis in which the reservoir solution is allowed to evaporate slowly. The slow increase in precipitant concentration allows crystals to grow without increasing nucleation. The method is useful for proteins that have a very narrow metastable zone (the range of solution conditions under which crystals grow but nuclei do not form at an appreciable rate). The method is demonstrated with the coat protein of potato virus X.
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Department of Molecular Biology, Vanderbilt University, Box 1820, Station B, Nashville, Tennessee 37235, USA.
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D002213:Capsid / Q000737:chemistry*; D036022:Capsid Proteins; D003460:Crystallization; D017551:Microdialysis / Q000379:methods*; D009113:Muramidase / Q000737:chemistry*
D036022:Capsid Proteins; C058750:coat protein, Potato virus X; D009113:Muramidase