Microbial degradation of phenanthrene by addition of a sophorolipid mixture.
The influence of sophorolipids on microbial degradation of poorly soluble phenanthrene in liquid and soil suspension culture was evaluated in the work presented. Experiments were carried out in two parts. In the first part, important basic physico-chemical characteristics of the biosurfactant and the pollutant used were determined. The critical micelle concentration (CMC) and the solubilization ratio of the biosurfactant were found to be in a good range compared with synthetic surfactants. Also, a reduction to 71% of the detectable amount of phenanthrene was measured within 4 d in soil suspension without any biotic influence. In the second part, culture experiments were done with Sphingomonas yanoikuyae, the bacterium used throughout the work presented here with the aim to assess the toxicity of the sophorolipids on these bacteria and the effect of the surfactant on biodegradation. In exponential growth tests, no toxicity up to 1 g l(-1) sophorolipids could be detected, whereas in an agar plate test, slight growth hindrance was measured at a lower concentration of 250 mg l(-1). The above mentioned data were important for planning further experiments. In the following cultivations with liquid and soil suspension media, enhancements of the biodegradation with surfactant addition were measurable. Fluorescence measurements showed that this effect was not due to an increasing biomass, but to an augmentation of bioavailability of the phenanthrene through increasing the apparent dissolved pollutant. Surfactant addition had the consequence of decreasing the residual detectable pollutant concentration (after 36 h 0.5 compared with 2.3 mg l(-1) soil suspension) and increasing the maximal degradation rate (127 instead of 80 mg l(-1) soil suspension x 10 h). Therefore, the two main problems of biological soil remediation techniques, longer process time and residual pollutants, may be solved by the use of surfactants.
Schippers
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Institut für Technische Chemie, Universität Hannover, Germany.
Gessner
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Müller
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Scheper
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D001673:Biodegradation, Environmental; D019149:Bioreactors; D003470:Culture Media; D010616:Phenanthrenes / Q000378:metabolism*; D012989:Soil Pollutants / Q000378:metabolism*; D012995:Solubility; D020578:Sphingomonas / Q000254:growth & development* / Q000378:metabolism*; D013501:Surface-Active Agents / Q000737:chemistry*
D003470:Culture Media; D010616:Phenanthrenes; D012989:Soil Pollutants; D013501:Surface-Active Agents; C031181:phenanthrene