title: Antigen-presenting capability of glial cells under glioma-harboring conditions and the effect of glioma-derived factors on antigen presentation.
abstract: The antigen-presenting capability of syngeneic rat glial cells was investigated under glioma-harboring conditions. Microglia induced a significant proliferation of glioma-primed splenocytes, but astrocytes did not. Furthermore, astrocytes suppressed the accessory cell function of microglia. The presence of both indomethacin and anti-interleukin (IL)-10 neutralizing antibody during priming of microglia enhanced splenocyte proliferation. The glioma culture supernatants down-regulated the interferon-gamma-induced expression of major histocompatibility complex class II molecules on microglia. The down-regulation was blocked by indomethacin and anti-IL-10 antibody. The results suggest that microglia but not astrocytes may function as antigen-presenting cells in glioma, and that glioma may suppress the antigen-presenting abilities of microglia.
authors.affiliation: Department of Neurosurgery, Brain Research Institute, Niigata University, Asahimachi 1, 951-8585, Niigata, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Taniguchi
authors.forename: K
authors.initials: K
authors.lastname: Ono
authors.forename: S
authors.initials: S
authors.lastname: Yoshida
authors.forename: R
authors.initials: R
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D000906:Antibodies / Q000494:pharmacology; D000938:Antigen-Presenting Cells / Q000737:chemistry / Q000276:immunology*; D001253:Astrocytes / Q000737:chemistry / Q000276:immunology*; D001932:Brain Neoplasms; D002455:Cell Division / Q000187:drug effects / Q000276:immunology; D017077:Culture Media, Conditioned / Q000494:pharmacology; D005260:Female; D005434:Flow Cytometry; D018316:Gliosarcoma; D000949:Histocompatibility Antigens Class II / Q000032:analysis / Q000276:immunology; D007213:Indomethacin / Q000494:pharmacology; D016753:Interleukin-10 / Q000276:immunology; D008297:Male; D017628:Microglia / Q000737:chemistry / Q000276:immunology*; D009500:Neutralization Tests; D011453:Prostaglandins / Q000276:immunology; D051381:Rats; D011916:Rats, Inbred F344; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000276:immunology
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000906:Antibodies; D017077:Culture Media, Conditioned; D000949:Histocompatibility Antigens Class II; D011453:Prostaglandins; D016753:Interleukin-10; D007213:Indomethacin