Use of whole blood for spectrophotometric determination of cholinesterase activity in dogs.
Whole blood has been compared with erythrocytes and plasma for spectrophotometric cholinesterase determination in the dog. Cholinesterase activity was characterized using two substrates: acetylthiocholine and butyrylthiocholine. Acetylcholinesterase was the only form of cholinesterase present on erythrocytes and hydrolysed only acetylthiocholine. Butyrylcholinesterase (pseudocholinesterase) was predominant in plasma, hydrolysing mainly butyrylthiocholine. Based on these results, a method based on the use of two substrates (acetylthiocholine for monitoring acetylcholinesterase and butyrylthiocholine for determining butyrylcholinesterase) in the same whole blood sample is recommended for canine cholinesterase analysis. This way of monitoring both enzymes can be easily automated, yielding good within (CVs < 5%) and between-run (CVs < 7%) precision.
Tecles
F
F
Department of Animal Pathology, Faculty of Veterinary Medicine, University of Murcia, 30100, Murcia, Spain. Ftecles@fcu.um.es
Martínez Subiela
S
S
Bernal
L J
LJ
Cerón
J J
JJ
D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives / Q000737:chemistry; D000122:Acetylthiocholine / Q000737:chemistry; D000818:Animals; D002092:Butyrylthiocholine / Q000737:chemistry; D002800:Cholinesterase Inhibitors / Q000737:chemistry; D002802:Cholinesterases / Q000097:blood*; D003372:Coumaphos / Q000737:chemistry; D004220:Disulfides / Q000737:chemistry; D004228:Dithionitrobenzoic Acid / Q000737:chemistry; D004285:Dogs / Q000097:blood*; D007300:Insect Control; D007306:Insecticides / Q000737:chemistry; D016877:Oxidants / Q000737:chemistry; D013439:Sulfhydryl Reagents / Q000737:chemistry
D002800:Cholinesterase Inhibitors; D004220:Disulfides; D007306:Insecticides; D016877:Oxidants; D013439:Sulfhydryl Reagents; C001105:2,2'-dipyridyl disulfide; D000122:Acetylthiocholine; D002092:Butyrylthiocholine; D015082:2,2'-Dipyridyl; D004228:Dithionitrobenzoic Acid; D002802:Cholinesterases; D003372:Coumaphos