title: Purification, chemical, and immunochemical properties of a new lectin from Mimosoideae (Parkia discolor).
abstract: A glucose/mannose-binding lectin was isolated from seeds of Parkia discolor (Mimosoideae) using affinity chromatography on Sephadex G-100 gel. The protein presented a unique component in SDS-PAGE corresponding to a molecular mass of 58,000 Da, which is very similar to that of a closely related lectin from Parkia platycephala. Among the simple sugars tested, mannose was the best inhibitor, but biantennary glycans, containing the trimannoside core, present in N-glycoproteins, also seem to be powerful inhibitors of the haemagglutinating activity induced by the purified lectin. The protein was characterised by high content of glycine and proline and absence of cysteine. Rabbit antibodies, anti-P. platycephala seed lectin, recognised the P. discolor lectin. However, no cross-reaction was observed when a set of other legume lectins from sub-family Papilionoideae and others from families Moraceae and Euphorbiaceae were assayed with the Parkia lectins. This suggests that Parkia lectins comprise a new group of legume lectins exhibiting distinct characteristics.
authors.affiliation: Departamento de Biologia, Universidade Federal do Ceará, Fortaleza-Ceará, Brasil.
authors.forename: B S
authors.initials: BS
authors.lastname: Cavada
authors.lastname: Madeira SVF
authors.forename: J J
authors.initials: JJ
authors.lastname: Calvete
authors.forename: L A
authors.initials: LA
authors.lastname: Souza
authors.forename: L R
authors.initials: LR
authors.lastname: Bomfim
authors.forename: A R
authors.initials: AR
authors.lastname: Dantas
authors.forename: M C
authors.initials: MC
authors.lastname: Lopes
authors.forename: T B
authors.initials: TB
authors.lastname: Grangeiro
authors.forename: B T
authors.initials: BT
authors.lastname: Freitas
authors.forename: V P
authors.initials: VP
authors.lastname: Pinto
authors.forename: K B
authors.initials: KB
authors.lastname: Leite
authors.forename: M V
authors.initials: MV
authors.lastname: Ramos
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D007887:Fabaceae / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D037102:Lectins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D008970:Molecular Weight; D037121:Plant Lectins; D010946:Plants, Medicinal; D011817:Rabbits
chemical_list: D000596:Amino Acids; D006023:Glycoproteins; D037102:Lectins; D037121:Plant Lectins