Effects of systemic injection of interleukin-1beta on gastric vagal afferent activity in rats lacking type A cholecystokinin receptors.
We have shown that systemic administration of interleukin-1beta (IL-1beta) excites gastric vagal afferent activity in part via stimulation of type A cholecystokinin (CCK-A) receptors in rats. The present study was undertaken to determine whether the response of the gastric vagal afferent nerve to systemic IL-1beta is altered in Otsuka Long-Evans Tokushima Fatty (OLETF) rats, which lack CCK-A receptors. The response was compared with that of the control strain, Long-Evans Tokushima Otsuka (LETO) rats. All animals were anesthetized with pentobarbital and artificially ventilated. Intravenous administration of 4 microg/kg of IL-1beta increased gastric vagal afferent activity in both LETO and OLETF rats, whereas a smaller dose of 2 microg/kg of IL-1beta increased activity only in the OLETF rats. The present results demonstrate that the response of the gastric vagal afferent activity in CCK-A receptor deficient OLETF rats was more sensitive to intravenous administration of IL-1beta than was in control LETO rats.
Kurosawa
M
M
Basic Medical Research Center, International University Health and Welfare, Otawara, Tochigi, Japan. mieko-ku@iuhw.ac.jp
Bucinskaite
V
V
Miyasaka
K
K
Funakoshi
A
A
Lundeberg
T
T
D000818:Animals; D007275:Injections, Intravenous; D007375:Interleukin-1 / Q000494:pharmacology*; D051379:Mice; D009475:Neurons, Afferent / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D020334:Rats, Inbred OLETF; D020318:Rats, Long-Evans; D011949:Receptors, Cholecystokinin / Q000172:deficiency*; D011994:Recombinant Proteins / Q000494:pharmacology; D012016:Reference Values; D013270:Stomach / Q000294:innervation*; D014630:Vagus Nerve / Q000187:drug effects / Q000502:physiology*
D007375:Interleukin-1; D011949:Receptors, Cholecystokinin; D011994:Recombinant Proteins