Exposure of brown trout, Salmo trutta, to a sub-lethal concentration of copper in soft acidic water: effects upon muscle metabolism and membrane potential.
Brown trout acclimated to soft water and exposed for 96 h to a sub-lethal concentration of copper at low pH (0.08 micromol l(-1) Cu, pH 5) have a lower critical swimming speed than fish from copper-free water at neutral pH. This loss of performance is not due to difficulties in oxygen transfer resulting from gill damage since arterial oxygen and carbon dioxide levels remain unaffected. Both red and white muscle showed some metabolic disruptions consistent with local hypoxia, namely a high lactate concentration at rest and, in the white muscle, depletion of glycogen and phosphocreatine. However, a putative role of increased blood viscosity following haematological changes in reducing the supply of oxygen to the tissues is not supported by the current study. Haematocrit, haemoglobin and plasma protein concentrations were not affected by this treatment and a lack of further change in variables such as lactate at the onset of exercise led one to look for an alternative explanation for the effects of copper and low pH upon tissue metabolites. Ammonia concentration, both in the plasma and muscles, is significantly higher in trout exposed to copper and low pH. Ammonia plays a role in the regulation of a number of metabolic pathways and could contribute to the altered metabolic status of these fish. In addition, ammonium ions are known to cause electrophysiological disruptions, particularly the displacement of K(+) in ion exchange mechanisms that could lead to the observed loss of swimming performance. Using the measured distribution of ammonia between intracellular and extracellular compartments to estimate membrane potential of resting muscle, a significant depolarisation is predicted in both red and white muscle of fish exposed to copper and low pH.
Beaumont
M W
MW
School of Biosciences, The University of Birmingham, Edgbaston, B15 2TT, Birmingham, UK. m.w.beaumont@bham.ac.uk
Butler
P J
PJ
Taylor
E W
EW
D000465:Algorithms; D000641:Ammonia / Q000378:metabolism; D000818:Animals; D002395:Catecholamines / Q000097:blood; D003300:Copper / Q000633:toxicity*; D005618:Fresh Water / Q000032:analysis; D005947:Glucose / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects; D010732:Phosphofructokinase-1 / Q000378:metabolism; D012487:Salmonidae / Q000502:physiology*; D013550:Swimming / Q000502:physiology
D002395:Catecholamines; D019344:Lactic Acid; D000641:Ammonia; D003300:Copper; D010732:Phosphofructokinase-1; D005947:Glucose