[The effect of PMN adhesion mediated by CD11b/CD18 on the increasing permeability of microvascular endothelial monolayer after severe burn injury].
OBJECTIVE
To detect the effect of burn-activated PMN adhesion and its adhesion molecule CD11b/CD18 on microvascular endothelial permeability using an experimental model of endothelial monolayer on polycarbonate microporous filters.


METHODS
An experimental model for in vitro study of endothelal monolayer for permeability analysis was established. Seven groups were divided into according to the treatment of microvascular endothelial monolayer. Fluid filtration coeffecient(Kf) and albumin reflection coeffecient(delta) were measured after endothelial monolayer was perfused with albumin labelled by FITC.


RESULTS
Burn-activated PMN could increase the level of fluid filtration coeffecient(Kf) and decrease the albumin reflection coeffecient(delta). Monoclonal antibody sealing off CD11b/CD18 on PMN provented the change of delta induced by burn-activated PMN. Another microporous filter interposed between PMNs and endothelial monolayer corrected the changes of Kf and delta.


CONCLUSIONS
The permeability enhancing effect of PMNs may be attributed mainly to the PMN-EC adhesion mediated by CD11b/CD18. Blocking the PMN-EC over-adhesion in moderation may be helpful in reducing the lung injury due to severe burn injury.
Fang
Y
Y
Burns Center, Changhai Hospital, Second Military Medical University, Shanghai.
Chen
Y
Y
Ge
S
S
D000818:Animals; D002056:Burns / Q000473:pathology*; D018821:CD18 Antigens / Q000494:pharmacology*; D002196:Capillaries / Q000473:pathology; D002199:Capillary Permeability / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000502:physiology; D004730:Endothelium, Vascular / Q000473:pathology*; D008168:Lung / Q000098:blood supply; D016177:Macrophage-1 Antigen / Q000494:pharmacology*; D008297:Male; D009504:Neutrophils / Q000502:physiology; D051381:Rats
D018821:CD18 Antigens; D015815:Cell Adhesion Molecules; D016177:Macrophage-1 Antigen