title: Comparative genomic hybridization reveals recurrent enhancements on chromosome 20 and in one case combined amplification sites on 15q24q26 and 20p11p12 in glioblastomas.
abstract: We examined homogenized tissue samples of biopsies from 19 astrocytomas of different grades for genetic imbalances using comparative genomic hybridization (CGH): three astrocytomas grade II, and 16 astrocytomas grade IV (glioblastoma multiforme), one of the glioblastomas representing the recurrence of a benign oligoastrocytoma. In two of three cases of astrocytoma grade II, a gain of chromosome 7 was found. The alterations in the glioblastomas were complex, and most frequently showed the characteristic gain of chromosome 7 and loss of chromosome 10. The single analyzed case of recurrence of an oligoastrocytoma was characterized by a unique CGH pattern. This tumor showed two distinct alterations: apart from an amplification on 15q24q26, we found a distinct amplification of a small region on 20p11.2p12, which has not been previously described in brain tumors. Partial or complete gains of chromosome 20 arose in six other tumors; we conclude that chromosome 20 in particular 20p11. 2p12, may harbor relevant genes for glioma progression.
authors.affiliation: Institute of Human Genetics, University of the Saarland, Homburg/Saar, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Brunner
authors.forename: V
authors.initials: V
authors.lastname: Jung
authors.forename: W
authors.initials: W
authors.lastname: Henn
authors.forename: K D
authors.initials: KD
authors.lastname: Zang
authors.forename: S
authors.initials: S
authors.lastname: Urbschat
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001932:Brain Neoplasms / Q000235:genetics*; D002884:Chromosomes, Human, Pair 15; D002890:Chromosomes, Human, Pair 20; D005260:Female; D005784:Gene Amplification; D005909:Glioblastoma / Q000235:genetics*; D006801:Humans; D007621:Karyotyping; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization / Q000379:methods*