title: Electrochemical studies of the effect of lanthanide ions on the activity of glutamate dehydrogenase.
abstract: Using the electrochemical method based on the "diffusion" layer concept of the convective system, the effect of lanthanide ions La3+ and Eu3+ on the activity of glutamate dehydrogenase (GLDH) has been determined. In suitable concentrations, the lanthanide ions La3+ and Eu3+ can activate the GLDH in the reductive amination of alpha-Ketoglutarate (alpha-KG). The activation mechanism would be that the lanthanide ions could interrupt the binding of NAD+ to GLDH by combining preferentially to NAD+. This mechanism was proposed by the voltammetric studies.
authors.affiliation: Department of Chemistry, Beijing, People's Republic of China. qkzhuang@chemms.chem.pku.edu.cn
authors.forename: Q K
authors.initials: QK
authors.lastname: Zhuang
authors.forename: H C
authors.initials: HC
authors.lastname: Dai
authors.forename: X X
authors.initials: XX
authors.lastname: Gao
authors.forename: W K
authors.initials: WK
authors.lastname: Xin
mesh_terms: D004563:Electrochemistry; D004789:Enzyme Activation; D005969:Glutamate Dehydrogenase / Q000378:metabolism*; D008674:Metals, Rare Earth / Q000494:pharmacology*; D009243:NAD / Q000378:metabolism; D011485:Protein Binding
chemical_list: D008674:Metals, Rare Earth; D009243:NAD; D005969:Glutamate Dehydrogenase