Determination of sugar alcohols in confectioneries by high-performance liquid chromatography after nitrobenzoylation.
A method was developed for the determination of sugar alcohols, meso-erythritol, xylitol, D-glucitol, D-mannitol, maltitol and parachinit by high-performance liquid chromatography (HPLC). The sugar alcohols were converted into strong ultraviolet (UV)-absorbing derivatives with p-nitrobenzoyl chloride. HPLC was performed on a phenyl column, using acetonitrile-water (67:33) as mobile phase and UV detection (260 nm). The calibration curves for all sugar alcohols tested were linear in the 10-250 microg/ml range. The average recoveries of the sugar alcohols from four sugarless confectioneries spiked at 5 and 10% levels of six sugar alcohol standards ranged from 73.2 to 109.0% with relative standard deviations ranging from 0.7 to 9.0%. The detection limit of the developed method was 0.1% for the above sugar alcohols contained in the samples.
Nojiri
S
S
Tama Branch Laboratory, Tokyo Metropolitan Research Laboratory of Public Health, Tachikawa, Japan.
Taguchi
N
N
Oishi
M
M
Suzuki
S
S
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005504:Food Analysis; D009579:Nitrobenzoates / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013402:Sugar Alcohols / Q000032:analysis*
D009579:Nitrobenzoates; D013402:Sugar Alcohols; C043240:4-nitrobenzoyl chloride