5 S ribosomal spacer sequences of some filarial parasites: comparative analysis and diagnostic applications.
We have amplified by PCR the sequences of the 5 S ribosomal spacer of Setaria labiatopapillosa and Foleyella furcata. After sequencing, these sequences have been compared with those of Dirofilaria immitis and Dirofilaria repens. Two major goals have been achieved: (i) the establishment of a multiplex PCR-based diagnostic assay, applicable to identify the four species in vertebrate and invertebrate hosts; (ii) the identification, in S. labiatopapillosa and F. furcata, of a canonical spliced leader 1 (SL1) sequence, so confirming that only D. repens, of the filarial parasites so far studied, shows a peculiar SL1 sequence. The PCR assay here developed and the analysis of the 5 S ribosomal spacer, can further improve both epidemiological and molecular analysis of these filarial species.
Favia
G
G
Istituto di Patologia Generale Veterinaria dell'Università degli Studi di Milano, via Celoria 10, Milano, 20133, Italy. favia@axrma.uniroma1.it
Cancrini
G
G
Ricci
I
I
Bazzocchi
C
C
Magi
M
M
Pietrobelli
M
M
Genchi
C
C
Bandi
C
C
D000818:Animals; D001483:Base Sequence; D021903:DNA, Ribosomal Spacer; D004182:Dirofilaria / Q000235:genetics* / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012689:Sequence Homology, Nucleic Acid; D017182:Setaria Nematode / Q000235:genetics / Q000302:isolation & purification; D013045:Species Specificity
D021903:DNA, Ribosomal Spacer