[Lipopolysaccharides of Shigella sonnei].
Immunobiological properties of native lipopolysaccharides (LPS) from virulent and avirulent strains of Shigella sonnei bacteria (LPS-V and LPS-A, respectively) were studied. In avirulent bacteria, LPS-V induced immunosuppressive activity specific of the virulent strain. LPS of the avirulent strain, whereas LPS-A lacked this property. Native LPS-V with immunosuppressive activity were isolated from the virulent strain by and immune affinity method. Treatment of LPS-V with phenol or TCA abolished its activity and converted it into the LPS-A form. The data showed that LPS-A can be converted back to the LPS-V form by redox treatment. This approach seems to be promising for activating LPS extracted from cells with TCA or a water-phenol mixture.
Borisova
E V
EV
T. Shevchenko Kiev University, Ukraine.
Borisov
V A
VA
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D004405:Dysentery, Bacillary / Q000276:immunology* / Q000382:microbiology; D006168:Guinea Pigs; D008070:Lipopolysaccharides / Q000276:immunology*; D012764:Shigella sonnei / Q000276:immunology* / Q000472:pathogenicity; D014774:Virulence / Q000276:immunology
D000942:Antigens, Bacterial; D008070:Lipopolysaccharides