title: Structure of a glycerol-conducting channel and the basis for its selectivity.
abstract: Membrane channel proteins of the aquaporin family are highly selective for permeation of specific small molecules, with absolute exclusion of ions and charged solutes and without dissipation of the electrochemical potential across the cell membrane. We report the crystal structure of the Escherichia coli glycerol facilitator (GlpF) with its primary permeant substrate glycerol at 2.2 angstrom resolution. Glycerol molecules line up in an amphipathic channel in single file. In the narrow selectivity filter of the channel the glycerol alkyl backbone is wedged against a hydrophobic corner, and successive hydroxyl groups form hydrogen bonds with a pair of acceptor, and donor atoms. Two conserved aspartic acid-proline-alanine motifs form a key interface between two gene-duplicated segments that each encode three-and-one-half membrane-spanning helices around the channel. This structure elucidates the mechanism of selective permeability for linear carbohydrates and suggests how ions and water are excluded.
authors.affiliation: Department of Biochemistry and Biophysics, School of Medicine, University of California, San Francisco, CA 94143-0448, USA.
authors.forename: D
authors.initials: D
authors.lastname: Fu
authors.forename: A
authors.initials: A
authors.lastname: Libson
authors.forename: L J
authors.initials: LJ
authors.lastname: Miercke
authors.forename: C
authors.initials: C
authors.lastname: Weitzman
authors.forename: P
authors.initials: P
authors.lastname: Nollert
authors.forename: J
authors.initials: J
authors.lastname: Krucinski
authors.forename: R M
authors.initials: RM
authors.lastname: Stroud
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D020346:Aquaporins / Q000737:chemistry / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000378:metabolism; D002463:Cell Membrane Permeability; D017124:Conserved Sequence; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000737:chemistry*; D029968:Escherichia coli Proteins; D005990:Glycerol / Q000737:chemistry / Q000378:metabolism*; D006860:Hydrogen Bonding; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D011510:Proteolipids / Q000378:metabolism; D013237:Stereoisomerism; D013402:Sugar Alcohols / Q000378:metabolism; D014867:Water / Q000378:metabolism
chemical_list: D020346:Aquaporins; D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; D011510:Proteolipids; D013402:Sugar Alcohols; C020485:proteoliposomes; D014867:Water; C078043:GlpF protein, E coli; D005990:Glycerol