Contributions of Torpedo nicotinic acetylcholine receptor gamma Trp-55 and delta Trp-57 to agonist and competitive antagonist function.
Results of affinity-labeling studies and mutational analyses provide evidence that the agonist binding sites of the nicotinic acetylcholine receptor (nAChR) are located at the alpha-gamma and alpha-delta subunit interfaces. For Torpedo nAChR, photoaffinity-labeling studies with the competitive antagonist d-[(3)H]tubocurarine (dTC) identified two tryptophans, gammaTrp-55 and deltaTrp-57, as the primary sites of photolabeling in the non-alpha subunits. To characterize the importance of gammaTrp-55 and deltaTrp-57 to the interactions of agonists and antagonists, Torpedo nAChRs were expressed in Xenopus oocytes, and equilibrium binding assays and electrophysiological recordings were used to examine the functional consequences when either or both tryptophans were mutated to leucine. Neither substitution altered the equilibrium binding of dTC. However, the deltaW57L and gammaW55L mutations decreased acetylcholine (ACh) binding affinity by 20- and 7,000-fold respectively. For the wild-type, gammaW55L, and deltaW57L nAChRs, the concentration dependence of channel activation was characterized by Hill coefficients of 1.8, 1.1, and 1.7. For the gammaW55L mutant, dTC binding at the alpha-gamma site acts not as a competitive antagonist but as a coactivator or partial agonist. These results establish that interactions with gamma Trp-55 of the Torpedo nAChR play a crucial role in agonist binding and in the agonist-induced conformational changes that lead to channel opening.
Xie
Y
Y
Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, USA.
Cohen
J B
JB
D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D001665:Binding Sites / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D015640:Ion Channel Gating; D009154:Mutation; D018722:Nicotinic Agonists / Q000378:metabolism*; D018733:Nicotinic Antagonists / Q000378:metabolism*; D019920:Photoaffinity Labels; D021122:Protein Subunits; D011978:Receptors, Nicotinic / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000187:drug effects / Q000378:metabolism; D014101:Torpedo; D014364:Tryptophan; D014403:Tubocurarine / Q000378:metabolism
D018722:Nicotinic Agonists; D018733:Nicotinic Antagonists; D019920:Photoaffinity Labels; D021122:Protein Subunits; D011978:Receptors, Nicotinic; D011994:Recombinant Proteins; D014364:Tryptophan; D000109:Acetylcholine; D014403:Tubocurarine