Dynamic interaction between myogenic and TGF mechanisms in afferent arteriolar blood flow autoregulation.
The dynamic activity of afferent arteriolar diameter (AAD) and blood flow (AABF) responses to a rapid step increase in renal arterial pressure (100-148 mmHg) was examined in the kidneys of normal Sprague-Dawley rats (n = 11) before [tubuloglomerular feedback (TGF)-intact] and after interruption of distal tubular flow (TGF-independent). Utilizing the in vitro blood-perfused juxtamedullary nephron preparation, fluctuations in AAD and erythrocyte velocity were sampled by using analog-to-digital computerized conversion, video microscopy, image shearing, and fast-frame, slow-frame techniques. These assessments enabled dynamic characterization of the autonomous actions and collective interactions between the myogenic and TGF mechanisms at the level of the afferent arteriole. The TGF-intact and TGF-independent systems exhibited common initial (0-24 vs. 0-13 s, respectively) response slope kinetics (-0.53 vs. -0.47% DeltaAAD/s; respectively) yet different maximum vasoconstrictive magnitude (-11.28 +/- 0.1 vs. -7. 02 +/- 0.9% DeltaAAD; P < 0.05, respectively). The initial AABF responses similarly exhibited similar kinetics but differing magnitudes. In contrast, during the sustained pressure input (13-97 s), the maximum vasoconstrictor magnitude (-7.02 +/- 0.9% DeltaAAD) and kinetics (-0.01% DeltaAAD/s) of the TGF-independent system were markedly blunted whereas the TGF-intact system exhibited continued vasoconstriction with slower kinetics (-0.20% DeltaAAD/s) until a steady-state plateau was reached (-25.9 +/- 0.4% DeltaAAD). Thus the TGF mechanism plays a role in both direct mediation of vasoconstriction and in modulation of the myogenic response.
Walker
M
M
Department of Physiology, Tulane University School of Medicine, New Orleans, Louisiana 70112, USA. mwalker3@tulane.edu
Harrison-Bernard
L M
LM
Cook
A K
AK
Navar
L G
LG
D000818:Animals; D001160:Arterioles / Q000502:physiology*; D001783:Blood Flow Velocity / Q000502:physiology; D001794:Blood Pressure / Q000502:physiology; D005246:Feedback / Q000502:physiology; D066298:In Vitro Techniques; D007678:Kidney Glomerulus / Q000098:blood supply* / Q000502:physiology; D007684:Kidney Tubules / Q000098:blood supply* / Q000502:physiology; D008297:Male; D008955:Models, Cardiovascular; D009399:Nephrons / Q000098:blood supply / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012044:Regression Analysis; D012079:Renal Circulation / Q000502:physiology*; D014654:Vascular Patency / Q000502:physiology; D014661:Vasoconstriction / Q000502:physiology