title: Sperm survival during short-term storage and after cryopreservation of semen from striped trumpeter (Latris lineata).
abstract: Methods of short-term storage and cryopreservation were examined for semen from striped trumpeter (Latris lineata). For fresh semen at 18 degrees C, the percentage of motile sperm declined rapidly from over 80% immediately after activation with sea water to less than 2% within 9 min after activation. The motility after activation of undiluted fresh sperm stored at 5 degrees C was maintained for two days and then declined markedly so that by the eighth day, sperm were mostly immotile after activation. The post-thawing motility was higher for sperm frozen with a non-activating diluent containing 2.84 M DMSO in saline (117 mM NaCl) than in an activating glycerol (2 M) medium in dilute sea water (300 mOsm). Post-thawing motility was higher for a dilution rate of 1:5 (semen:diluent) than 1:2 or 1:11 but was similar when frozen semen was thawed at 10 degrees, 20 degrees or 30 degrees C. For semen stored at a range of volumes as pellets frozen on dry ice (0.2 to 2.0 mL) or straws frozen in liquid nitrogen vapor (0.25 to 0.5 mL) and thawed in a waterbath at 20 degrees C, the post-thawing motilities were similar even though the patterns of cooling and thawing differed markedly between methods of freezing and sizes of pellets and straws.
authors.affiliation: Tasmanian Aquaculture and Fisheries Institute, University of Tasmania, Hobart, Australia. Arthur.Ritar@utas.edu.au
authors.forename: A J
authors.initials: AJ
authors.lastname: Ritar
authors.forename: M
authors.initials: M
authors.lastname: Campet
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000662:veterinary; D005399:Fishes / Q000502:physiology*; D007315:Insemination, Artificial / Q000662:veterinary; D008297:Male; D012662:Semen Preservation / Q000662:veterinary*; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology*; D013997:Time Factors