The effect of serum albumin on the aggregation state and toxicity of amphotericin B.
Studies have shown that the dose-limiting toxicity of amphotericin B (AmB), a key drug for systemic mycoses, depends on its self-aggregation state. In a step toward understanding the various factors in blood mediating the toxicity of AmB, we have investigated the effect of serum albumin, the most abundant plasma protein, on the aggregation state of AmB using absorption spectroscopy. The critical aggregation concentration (CAC) of AmB, which coincides with its concentration at the onset of toxicity (hemolysis), was 1.1 microM, but rose in proportion to the level of serum albumin (1.0 to 4.0% w/v). The CAC of AmB was 8.0 microM at 4.0% w/v serum albumin, which is considerably higher than peak therapeutic levels of AmB in plasma (i.e., 2.0 microM). Serum albumin (4.0% w/v) lowered the degree of aggregation of AmB (size of aggregates) above the CAC and increased its solubility. The results suggest that serum albumin attenuates the toxicity of AmB at a membrane level by affecting its aggregation state. In this way, serum albumin in blood may balance deleterious effects of AmB mediated by serum low-density lipoproteins.
Aramwit
P
P
School of Pharmacy, University of Wisconsin at Madison, Madison, Wisconsin 53706-1515, USA.
Yu
B G
BG
Lavasanifar
A
A
Samuel
J
J
Kwon
G S
GS
D000042:Absorption; D000666:Amphotericin B / Q000737:chemistry* / Q000633:toxicity*; D000818:Animals; D000935:Antifungal Agents / Q000737:chemistry* / Q000633:toxicity*; D002417:Cattle; D004121:Dimethyl Sulfoxide; D006461:Hemolysis; D066298:In Vitro Techniques; D008297:Male; D012709:Serum Albumin / Q000737:chemistry* / Q000494:pharmacology*; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet
D000935:Antifungal Agents; D012709:Serum Albumin; D012997:Solvents; D000666:Amphotericin B; D004121:Dimethyl Sulfoxide