Cloning of TC-1 (C8orf4), a novel gene found to be overexpressed in thyroid cancer.
A novel gene highly expressed in thyroid cancer, designated TC-1 (thyroid cancer-1), was cloned from suppression subtractive hybridization between papillary thyroid carcinoma and its surrounding normal thyroid tissue. Overexpression of TC-1 in thyroid cancer was confirmed in 15/16 paired samples by RT-PCR and Northern analysis. Ubiquitously expressed in human tissues, the TC-1 sequence showed no homology to any known gene, but matched a cluster of ESTs. After alignment of our sequence with the ESTs, the missing transcription start site was obtained by 5'-RACE and verified by primer extension analysis. The full-length mRNA sequence of 1327 bp has an open reading frame of 321 bp, which encodes a highly conserved protein. Three regulatory motifs were identified at the expected positions within 1 kb of the 5' flanking sequence obtained by genome walking. Using fluorescence in situ hybridization, TC-1 was localized to chromosome 8p11.2. The overexpression of TC-1 in papillary carcinoma suggests that it may have an important role in thyroid carcinogenesis.
Chua
E L
EL
Department of Medicine, University of Sydney, Sydney, New South Wales, 2006, Australia. echua@earth.endocrin.usyd.edu.au
Young
L
L
Wu
W M
WM
Turtle
J R
JR
Dong
Q
Q
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002645:Chickens; D002874:Chromosome Mapping / Q000379:methods; D002898:Chromosomes, Human, Pair 8; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005091:Exons; D015870:Gene Expression; D006801:Humans; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000145:classification / Q000235:genetics*; D012333:RNA, Messenger; D020539:Sequence Analysis, Protein / Q000379:methods; D017386:Sequence Homology, Amino Acid; D013964:Thyroid Neoplasms / Q000235:genetics*
D000596:Amino Acids; D018076:DNA, Complementary; D009363:Neoplasm Proteins; D012333:RNA, Messenger; C418741:TCIM protein, human