Establishment of a canine monocyte cell line.
A canine monocyte cell line was established from the peripheral blood sample collected from a healthy young male Beagle dog. The cloned cells grew easily and were serially passaged in vitro in the medium, a slight modification further made on Dulbecco's modified Eagle medium, supplemented with 10% fetal bovine serum. The morphology of single cell was shown in triangular or round form, however, it became epithelioid in a densely grown monolayer. Non-specific esterase was detected in all cells by a cytochemical examination. The cells reacted rapidly to the addition of a small amount of LPS and differentiated to the cells of morphologically typical macrophages. Both complement receptor (CD35) and Fc gamma receptor (CD64) were demonstrated on the cell membrane.
Kadoi
K
K
Laboratory of Bio-medical Science, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan.
D000818:Animals; D015703:Antigens, CD; D002454:Cell Differentiation; D002460:Cell Line; D002999:Clone Cells; D004285:Dogs; D007621:Karyotyping; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D009000:Monocytes / Q000166:cytology* / Q000187:drug effects; D010587:Phagocytosis
D015703:Antigens, CD; D008070:Lipopolysaccharides