Amount of dietary fat and type of soluble fiber independently modulate postabsorptive conversion of beta-carotene to vitamin A in mongolian gerbils.
Current dietary guidelines recommend a decrease in fat intake and an increase in fiber consumption. Decreased bioavailability (BV) of carotenoids is thought to be associated with both of these recommendations. A 2 x 4 factorial design was used to test the effects of dietary fat level at 10 or 30% of total energy and fiber type using no fiber, silica, citrus pectin or oat gum (7 g/100 g) on beta-carotene (betaC) BV in 4- to 5-wk-old Mongolian gerbils. We assessed BV as both accumulation of betaC and bioconversion of betaC to vitamin A (VA) in tissues. A VA- and betaC-deficient diet was fed for 1 wk followed by one of eight isocaloric, semipurified diets supplemented with carrot powder [ approximately 1 microgram betaC, 0.5 microgram alpha-carotene (alphaC)/kJ diet] for 2 wk (n = 12/group). Increasing dietary fat resulted in higher VA (P: = 0.074) and lower betaC (P: = 0.0001) stores in the liver, suggesting that consumption of high fat diets enhances conversion of betaC to VA. The effect of soluble fiber on hepatic VA storage was dependent on fiber type. Consumption of citrus pectin resulted in lower hepatic VA stores and higher hepatic betaC stores compared with all other groups, suggesting less conversion of betaC to VA. In contrast, consumption of oat gum resulted in hepatic VA and betaC stores that were higher (P = 0.012) and lower (P = 0.022), respectively, than those of citrus pectin-fed gerbils. The level of dietary fat consumed with soluble fiber had no interactive effects on hepatic VA, betaC or alphaC stores. Results demonstrate that betaC BV is independently affected by dietary fat level and type of soluble fiber, and suggest that these dietary components modulate postabsorptive conversion of betaC to VA. This study confirms the negative effects of citrus pectin on betaC BV, and suggests that oat gum does not adversely affect betaC BV.
Deming
D M
DM
Division of Nutritional Sciences, Department of Food Science and Human Nutrition, University of Illinois, Urbana, IL 61801, USA.
Boileau
A C
AC
Lee
C M
CM
Erdman
J W
JW
D000704:Analysis of Variance; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004032:Diet; D004041:Dietary Fats / Q000494:pharmacology*; D004043:Dietary Fiber / Q000494:pharmacology*; D005849:Gerbillinae; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D012995:Solubility; D014801:Vitamin A / Q000378:metabolism*; D015430:Weight Gain / Q000187:drug effects; D019207:beta Carotene / Q000378:metabolism*
D004041:Dietary Fats; D004043:Dietary Fiber; D019207:beta Carotene; D014801:Vitamin A