title: Analysis of the role of recA in phenotypic switching of Pseudomonas tolaasii.
abstract: Switching between the pathogenic smooth (1116S) and nonpathogenic rough (1116R) forms of Pseudomonas tolaasii occurs due to the reversible duplication of a 661-bp element within the pheN locus. Disruption of the chromosomal recA locus of 1116S and 1116R produced strains 1116SrecA and 1116RrecA, respectively, which showed typical loss of UV resistance. Switching from the smooth to the rough form was virtually eliminated in the 1116SrecA strain, whereas the extent of switching from the rough to the smooth form was almost identical in 1116R and 1116RrecA. It is concluded that phenotypic switching from 1116S to 1116R is recA dependent whereas that from 1116R to 1116S is recA independent.
authors.affiliation: Department of Plant Sciences, University of Cambridge, Cambridge CB2 3EA, United Kingdom.
authors.forename: H
authors.initials: H
authors.lastname: Sinha
authors.forename: A
authors.initials: A
authors.lastname: Pain
authors.forename: K
authors.initials: K
authors.lastname: Johnstone
mesh_terms: D000364:Agaricus; D002876:Chromosomes, Bacterial / Q000235:genetics; D017353:Gene Deletion; D009154:Mutation; D010641:Phenotype; D010935:Plant Diseases; D011549:Pseudomonas / Q000737:chemistry / Q000166:cytology / Q000235:genetics*; D011938:Rec A Recombinases / Q000235:genetics*; D011995:Recombination, Genetic
chemical_list: D011938:Rec A Recombinases