title: A mutation affecting the association equilibrium of formyltransferase from the hyperthermophilic Methanopyrus kandleri and its influence on the enzyme's activity and thermostability.
abstract: Formyltransferase from Methanopyrus kandleri is composed of only one type of subunit of molecular mass 32 kDa. The enzyme is in a monomer/dimer/tetramer association equilibrium, the association constant being affected by lyotropic salts. Oligomerization is required for enzyme activity and thermostability. We report here on a subunit interface mutation (R261E) which affects the dimer/tetramer part of the association equilibrium of formyltransferase. With the mutant protein it was shown that tetramerization is not required for activity but is necessary for high thermostability.
authors.affiliation: Max-Planck-Institut für terrestrische Mikrobiologie and Laboratorium für Mikrobiologie des Fachbereichs Biologie der Philipps-Universität, Marburg, Germany. shima@mailer.uni-marburg.de
authors.forename: S
authors.initials: S
authors.lastname: Shima
authors.forename: R K
authors.initials: RK
authors.lastname: Thauer
authors.forename: U
authors.initials: U
authors.lastname: Ermler
authors.forename: H
authors.initials: H
authors.lastname: Durchschlag
authors.forename: C
authors.initials: C
authors.lastname: Tziatzios
authors.forename: D
authors.initials: D
authors.lastname: Schubert
mesh_terms: D019943:Amino Acid Substitution; D001105:Archaea / Q000201:enzymology; D001120:Arginine; D019281:Dimerization; D004795:Enzyme Stability; D018698:Glutamic Acid; D019877:Hydroxymethyl and Formyl Transferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D011487:Protein Conformation; D021122:Protein Subunits; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013816:Thermodynamics
chemical_list: D021122:Protein Subunits; D011994:Recombinant Proteins; D018698:Glutamic Acid; D001120:Arginine; D019877:Hydroxymethyl and Formyl Transferases