[Alien chromosomal identification in VE161 wheat].
VE161 wheat which has the function of promotting homoeologous pairing is a male sterile substitution line or a fertile addition line with one pair of alien chromosomes from Agropyron elongatum. The alien chromosome in VE161 wheat was detected by means of techniques of total genomic DNA in situ hybridization, RFLP and double-ditelo chromosomal analysis. The results from in situ hybridization confirmed that the alien chromosome was derived from Agropyron elongatum. The RFLP analysis showed that it belongs to fourth homoeologous group, and the results from double-ditelo chromosomal analysis gave evidence that 4B chromosome was replaced in the substitution line, so it can be concluded that, in VE161 wheat, the alien chromosome would be 4E and the replaced wheat chromosome would be 4B in the substitution line. The reason for the replaced chromosome identified as 7B in a earlier study might be due to the rearrangement occurred in the genome of wheat.
Yang
T Z
TZ
Northwestern Agricultural University, Yangling, Shaanxi, China.
Ma
X F
XF
Zhang
W J
WJ
Yang
C L
CL
Hou
W S
WS
Bie
R L
RL
D002875:Chromosomes; D017403:In Situ Hybridization; D012150:Polymorphism, Restriction Fragment Length; D014908:Triticum / Q000235:genetics*