title: Staurosporine promotion of formation of continuous monolayers of primary rat hepatocytes by improving attachment and spreading.
abstract: Primary rat hepatocytes form discontinuous monolayers even at their maximum density. Here, we show that staurosporine promotes attachment and spreading of hepatocytes onto culture substrates, so that hepatocytes form a close, continuous monolayer. This treatment did not attenuate major hepatic functions. Therefore, this technique is promising for making seamless cell-sheet structures, which will be applicable for cell-polarity experiments or artificial liver construction.
authors.affiliation: Department of Chemistry, National Industrial Research Institute of Nagoya, Japan. smaeda@nirin.go.jp
authors.forename: S
authors.initials: S
authors.lastname: Maeda
authors.forename: K H
authors.initials: KH
authors.lastname: Lin
authors.forename: H
authors.initials: H
authors.lastname: Inagaki
authors.forename: T
authors.initials: T
authors.lastname: Saito
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects*; D018929:Cell Culture Techniques / Q000379:methods; D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D007700:Kinetics; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D019311:Staurosporine / Q000494:pharmacology*
chemical_list: D019311:Staurosporine