Highly sensitive and rapid detection of antibody catalysis by luminescent bacteria.
A highly sensitive, inexpensive, and facile bioluminescent assay for the detection of catalytic antibodies has been developed. This assay may be used for the early detection of antibody catalysis. The efficiency of this technique was exemplified by the use of the luminescent bacterium VhM42 for monitoring an antibody-catalyzed retroaldol fragmentation reaction with aldolase antibodies 38C2 and 24H6.
Shulman
H
H
Department of Chemistry and Institute of Catalysis Science and Technology, Technion-Israel Institute of Technology, Technion City, Haifa, Israel.
Eberhard
A
A
Eberhard
C
C
Ulitzur
S
S
Keinan
E
E
D000447:Aldehydes / Q000378:metabolism; D017151:Antibodies, Catalytic / Q000032:analysis* / Q000378:metabolism; D002138:Calibration; D002384:Catalysis; D005634:Fructose-Bisphosphate Aldolase / Q000276:immunology*; D007700:Kinetics; D049449:Luminescence; D012680:Sensitivity and Specificity; D014733:Vibrio / Q000378:metabolism*
D000447:Aldehydes; D017151:Antibodies, Catalytic; D005634:Fructose-Bisphosphate Aldolase