title: Ultrastructural changes in human gingival fibroblasts in vitro after exposure to vapour phase smoke components.
abstract: Tobacco and some of its volatile and non-volatile components have been found to affect many types of cells including gingival fibroblasts. Because normal gingival fibroblast functioning is fundamental to the maintenance of the oral connective tissue as well as to wound healing, we examined the effect of two vapour phase smoke components (acrolein and acetaldehyde) on proliferation and ultrastructure of human gingival fibroblasts (HGFs) in culture. A human gingival fibroblast strain derived from healthy individuals was used in this study. The cells were incubated in the presence of different concentrations of acrolein and acetaldehyde and cell proliferation and fine morphology were evaluated. The results show that acrolein and acetaldehyde produced dose dependent inhibition of HGF viability and alteration of cytoplasmic organelles. The main ultrastructural finding for the HGF cytoplasm was the presence of vacuoles and lysosomal structures which became prominent with increasing concentration of acrolein and acetaldehyde. Our results suggest that the ultrastructural alterations we observed in HGFs may be due to the uptake and storage of acrolein and acetaldehyde by the cells.
authors.affiliation: Department of Experimental Medicine, Faculty of Medicine, University of Pavia, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Poggi
authors.forename: M T
authors.initials: MT
authors.lastname: Rota
authors.forename: M G
authors.initials: MG
authors.lastname: Cusella De Angelis
authors.forename: R
authors.initials: R
authors.lastname: Boratto
mesh_terms: D000079:Acetaldehyde / Q000494:pharmacology*; D000171:Acrolein / Q000494:pharmacology*; D008841:Actin Cytoskeleton / Q000187:drug effects / Q000648:ultrastructure; D005347:Fibroblasts / Q000187:drug effects / Q000648:ultrastructure*; D005881:Gingiva / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D006801:Humans; D011132:Polyribosomes / Q000187:drug effects / Q000648:ultrastructure; D012016:Reference Values; D012906:Smoke; D014617:Vacuoles / Q000187:drug effects / Q000648:ultrastructure
chemical_list: D012906:Smoke; D000171:Acrolein; D000079:Acetaldehyde