Marked differences in quantity of infectious human immunodeficiency virus type 1 detected in persons with controlled plasma viremia by a simple enhanced culture method.
Culture of autologous CD4 lymphocytes from peripheral blood mononuclear cells compared favorably with two other methods for the measurement of cell-associated human immunodeficiency virus type 1 (HIV-1). For subjects with undetectable HIV-1 RNA levels in plasma, there was a 10,000-fold range of cell-associated virus detected. This method provides a simple and reproducible means for monitoring cell-associated HIV-1.
Tremblay
C L
CL
AIDS Research Center, Infectious Diseases Unit and Immunopathology Unit, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA.
Giguel
F
F
Merrill
D P
DP
Wong
J T
JT
Rosenberg
E
E
Kalams
S
S
Walker
B D
BD
D'Aquila
R T
RT
Hirsch
M S
MS
D015496:CD4-Positive T-Lymphocytes / Q000502:physiology / Q000821:virology*; D002478:Cells, Cultured; D016655:HIV Core Protein p24 / Q000032:analysis; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000302:isolation & purification* / Q000502:physiology*; D006801:Humans; D012367:RNA, Viral / Q000097:blood; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019562:Viral Load; D014766:Viremia / Q000821:virology; D014776:Virus Cultivation
D016655:HIV Core Protein p24; D012367:RNA, Viral