Comparison of STAT5 mRNA levels in GH-treated male and female rats analysed by a solution hybridization assay.
In this study we describe the development of a RNA:RNA solution hybridization-RNase protection assay to quantify STAT5 mRNA in total RNA extracts from rat tissues. The assay is sensitive and reproducible. We quantified STAT5 mRNA levels in liver and thymus lymphocytes from male and female control rats and from rats treated with a single dose of recombinant human growth hormone (rhGH). No significant sex differences in the expression pattern were observed in both studied tissues, but STAT5 mRNA levels were significantly (P< 0.05) higher in liver than in thymus lymphocytes. STAT5 mRNA levels were significantly (P< 0.05) increased by a pulse of GH given to either male or female normal rats, suggesting a regulation of STAT5 gene expression in the studied tissues. In conclusion, quantitative solution hybridization-RNase protection assay of STAT5 mRNA provides a tool to further advance the study of the regulatory mechanisms involved in STAT5 gene expression.
Ortiz
B L
BL
Department of Chemistry, Universidad Nacional de Colombia, Santafé de Bogotá, Colombia.
Sánchez-Gómez
M
M
Norstedt
G
G
D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D019382:Human Growth Hormone / Q000494:pharmacology*; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008297:Male; D008894:Milk Proteins; D009693:Nucleic Acid Hybridization / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D012260:Ribonucleases; D050799:STAT5 Transcription Factor; D012680:Sensitivity and Specificity; D012727:Sex Characteristics; D013601:T-Lymphocytes / Q000378:metabolism*; D013950:Thymus Gland / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects*
D004268:DNA-Binding Proteins; D008894:Milk Proteins; D012333:RNA, Messenger; D050799:STAT5 Transcription Factor; D015534:Trans-Activators; D019382:Human Growth Hormone; D012260:Ribonucleases