Expression of microglial response factor-1 in microglia and macrophages following cerebral ischemia in the rat.
Microglial response factor-1 is a newly isolated microglial gene, which encodes a Ca(2+) binding protein MRF-1 expressed in microglia and macrophages. We induced 1 h of focal cerebral ischemia or 10 min of global cerebral ischemia in the rat, and investigated the expression of MRF-1 immunoreactivity following ischemia. MRF-1 was present in resting microglia and was upregulated in response to microglial activation. MRF-1 was localized to all the cells of the mononuclear phagocyte system (microglia, monocytes, and perivascular cells) that appeared in the ischemic brain.
Kato
H
H
Department of Neurology, Field of Neuroscience, Tohoku University Graduate School of Medicine, 1-1 Seiryo-machi, Aoba-ku, 980-8574, Sendai, Japan. katoh@mail.cc.tohoku.ac.jp
Tanaka
S
S
Oikawa
T
T
Koike
T
T
Takahashi
A
A
Itoyama
Y
Y
D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism*; D002135:Calcium-Binding Proteins; D020244:Infarction, Middle Cerebral Artery / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D008840:Microfilament Proteins; D017628:Microglia / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
C100745:Aif1 protein, rat; D002135:Calcium-Binding Proteins; D008840:Microfilament Proteins; D009419:Nerve Tissue Proteins