title: An animal model for anaplastic large cell lymphoma in the immunocompetent syngeneic C57Bl/6 mouse.
abstract: We report on the analysis of a murine anaplastic lymphoid cell line TS1G6, established recently by interleukin (IL)-9 transfection. TS1G6 revealed a highly characteristic pattern of large anaplastic cells with mononuclear, binuclear, or multinuclear cells resembling Hodgkin (H) or Sternberg-Reed (SR) cells. This cell line is tumorigenous after injection of as few as 10(4) lymphoma cells into nude or immunocompetent C57Bl/6 mice and leads to death from progressive disease of all treated animals within a few weeks. The histological analysis of these tumors revealed a diffuse large cell malignant lymphoma that is morphologically almost identical to human anaplastic large cell lymphoma (ALCL). The lymphoma cells did not show overexpression of the anaplastic lymphoma kinase (ALK) gene, which is found in about 50% of the cases of human ALCL. Thus, this model may be an animal model for an important subset of human ALCL. The cytokine profile, which is of the T helper 2 type, showed strong parallels to the human lymphoma counterpart. Mice suffering from such lymphomas could not be cured with a regimen using high dose cyclophosphamide similar to many ALCL patients. Such an animal model for ALCL has not yet been recognized, but may provide the basis for investigating new antitumor immunotherapies in a fully immunocompetent host.
authors.affiliation: Department of Pathology, Medical University of Luebeck, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Bittner
authors.forename: A C
authors.initials: AC
authors.lastname: Feller
authors.forename: J C
authors.initials: JC
authors.lastname: Renauld
authors.forename: K
authors.initials: K
authors.lastname: Lange
authors.forename: R
authors.initials: R
authors.lastname: Pietrzik
authors.forename: C
authors.initials: C
authors.lastname: Jenetzky
authors.forename: J
authors.initials: J
authors.lastname: Briese
authors.forename: T
authors.initials: T
authors.lastname: Gaiser
authors.forename: A
authors.initials: A
authors.lastname: Müller
authors.forename: G J
authors.initials: GJ
authors.lastname: Wiedemann
authors.forename: J
authors.initials: J
authors.lastname: Van Snick
authors.forename: H
authors.initials: H
authors.lastname: Merz
mesh_terms: D000077548:Anaplastic Lymphoma Kinase; D000818:Animals; D003520:Cyclophosphamide / Q000627:therapeutic use; D016207:Cytokines / Q000235:genetics; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017730:Ki-1 Antigen / Q000032:analysis; D016403:Lymphoma, Large B-Cell, Diffuse / Q000188:drug therapy / Q000276:immunology / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D011505:Protein-Tyrosine Kinases / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D020794:Receptor Protein-Tyrosine Kinases; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D016207:Cytokines; D017730:Ki-1 Antigen; D012333:RNA, Messenger; D003520:Cyclophosphamide; C000626173:ALK protein, human; C000626174:Alk protein, mouse; D000077548:Anaplastic Lymphoma Kinase; D011505:Protein-Tyrosine Kinases; D020794:Receptor Protein-Tyrosine Kinases