title: Detection of Legionella pneumophila using a real-time PCR hybridization assay.
abstract: A real-time PCR hybridization assay for Legionella pneumophila is described; the assay uses LightCycler (Idaho Technology) methodology to specifically detect 2.5 CFU/reaction, equivalent to 1,000 CFU/liter of starting water sample. The assay, including DNA extraction and confirmation of product identity, is completed within 90 min of receipt of a sample.
authors.affiliation: Department of Microbiology and PHLS Laboratory, University Hospital, Nottingham NG7 2UH, United Kingdom.
authors.forename: A L
authors.initials: AL
authors.lastname: Ballard
authors.forename: N K
authors.initials: NK
authors.lastname: Fry
authors.forename: L
authors.initials: L
authors.lastname: Chan
authors.forename: S B
authors.initials: SB
authors.lastname: Surman
authors.forename: J V
authors.initials: JV
authors.lastname: Lee
authors.forename: T G
authors.initials: TG
authors.lastname: Harrison
authors.forename: K J
authors.initials: KJ
authors.lastname: Towner
mesh_terms: D004269:DNA, Bacterial / Q000032:analysis; D016952:Legionella pneumophila / Q000235:genetics / Q000302:isolation & purification*; D007877:Legionnaires' Disease / Q000382:microbiology / Q000517:prevention & control* / Q000635:transmission; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial