Effects of nerve growth factor on antioxidative system in the thalamus of MPTP treated Wistar rats.
1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced parkinsonism is one of the most useful models for the study of that disease. It has been suggested that MPTP-induced neurotoxicity may involve the production of reactive oxygen species. MPTP was applied intracerebrally, unilaterally, in the striatum in single dose of 0.09 g/kg b.w. The second group was treated both with MPTP and nerve growth factor (NGF) in dose of 7 ng/ml. NGF was applied immediately after the neurotoxin. Control group was treated with 0.9% saline solution in the same manner. Animals were decapitated 7 days after the treatment. In the group treated with MPTP, the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) was decreased in ipsilateral thalamus, compared to control values as well as to the contralateral thalamus. In the same structures superoxide anion production was increased, compared to controls. Following the application of both MPTP and NGF, the activity of SOD and GSH-Px remained on control values, while the superoxide anion content was decreased, compared to controls. These results indicate a temporal and spatial propagation of oxidative stress and spread protective effects of NGF on the thalamus, the structure that is distant, but very tightly connected with striatum, the place of direct neurotoxic damage.
Ninković
M B
MB
Military Medical Academy, Institute for Medical Research, Belgrade.
Jovanović
M D
MD
Malicević
Z
Z
Dukić
M
M
Jelenković
A
A
Mihajlović
R
R
Vasiljević
I
I
Jovicić
A
A
D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D000818:Animals; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D020932:Nerve Growth Factor / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D020734:Parkinsonian Disorders / Q000139:chemically induced / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D013788:Thalamus / Q000378:metabolism*
D013481:Superoxides; D020932:Nerve Growth Factor; D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase