Rats transgenic for human renin and human angiotensinogen as a model for gestational hypertension.
Animal models of gestational hypertension are problematic. A novel mouse model was described earlier. The dams in that study were transgenic for human angiotensinogen and the sires for human renin; human renin was expressed in and produced by the placenta. This model was adapted to the rat, which has greater utility in terms of chronic instrumentation and physiologic measurements. Female rats transgenic for human angiotensinogen were mated with rats transgenic for human renin. Telemetry BP increased on day 5 of pregnancy from 110/80 mmHg to as high as 180/140 mmHg, while heart rate increased slightly. The renin transgene was expressed in the placenta, which resulted in increased human plasma renin concentration from 0 to 937 +/- 800 ng angiotensin I ml/h; the values returned to 0 after delivery. Female rats transgenic for human renin that were mated with male rats transgenic for human angiotensinogen in contrast exhibited a decrease in BP. In these rats, human angiotensinogen in plasma remained undetectable. Double transgenic offspring of these transgenic rats developed hypertension and end-organ damage, regardless of the source of the transgenes. The conclusion is that transgenic rats that bear human renin and angiotensinogen genes make an attractive model for gestational hypertension. The rat model will have greater utility than the mouse model.
Bohlender
Jürgen
J
Franz Volhard Clinic, Berlin, Germany.|Max Delbrück Center, Medical Faculty of the Charié, Humboldt University of Berlin, Berlin, Germany.
Ganten
Detlev
D
Max Delbrück Center, Medical Faculty of the Charié, Humboldt University of Berlin, Berlin, Germany.|Department of Clinical Pharmacology, Klinikum Benjamin Franklin, Free University of Berlin, Berlin, Germany.
Luft
Friedrich C
FC
Franz Volhard Clinic, Berlin, Germany.|Max Delbrück Center, Medical Faculty of the Charié, Humboldt University of Berlin, Berlin, Germany.
D000809:Angiotensins / Q000097:blood / Q000235:genetics*; D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics; D000831:Animals, Newborn / Q000378:metabolism; D001794:Blood Pressure; D004195:Disease Models, Animal; D005260:Female; D005318:Fetal Heart / Q000473:pathology; D006801:Humans; D006973:Hypertension / Q000235:genetics* / Q000503:physiopathology; D008099:Liver / Q000378:metabolism; D009206:Myocardium / Q000473:pathology; D009929:Organ Size; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D011248:Pregnancy Complications / Q000097:blood / Q000503:physiopathology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012083:Renin / Q000097:blood / Q000235:genetics*
D000809:Angiotensins; D012333:RNA, Messenger; D012083:Renin