title: Identification of an ancillary protein, YabF, required for activity of the KefC glutathione-gated potassium efflux system in Escherichia coli.
abstract: A new subunit, YabF, for the KefC K(+) efflux system in Escherichia coli has been identified. The subunit is required for maximum activity of KefC. Deletion of yabF reduces KefC activity 10-fold, and supply of YabF in trans restores activity. IS2 and IS10R insertions in yabF can be isolated as suppressors of KefC activity consequent upon the V427A and D264A KefC mutations.
authors.affiliation: Department of Molecular and Cell Biology, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, United Kingdom.
authors.forename: S
authors.initials: S
authors.lastname: Miller
authors.forename: L S
authors.initials: LS
authors.lastname: Ness
authors.forename: C M
authors.initials: CM
authors.lastname: Wood
authors.forename: B C
authors.initials: BC
authors.lastname: Fox
authors.forename: I R
authors.initials: IR
authors.lastname: Booth
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins; D017353:Gene Deletion; D005798:Genes, Bacterial; D016153:Genes, Suppressor; D005978:Glutathione / Q000378:metabolism; D009154:Mutation; D015221:Potassium Channels / Q000235:genetics* / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D015221:Potassium Channels; C069287:KefC protein, E coli; D005978:Glutathione