Losses of heterozygosity in endometrial adenocarcinomas: positive correlations with histopathological parameters.
We analyzed 37 samples of endometrial adenocarcinoma for loss of heterozygosity (LOH) by using a panel of 44 microsatellites located in 29 chromosomal regions. The aim of our study was to investigate the existence of a possible preferential involvement of some tumor suppressor genes in endometrial carcinogenesis. The analysis was performed on tumoral tissue and on a corresponding normal tissue by the use of polymerase chain reaction (PCR) and the comparison of the amplified alleles. We observed significative LOH (>20%) in the chromosomal regions of 2q14 (33.33%), 7q35 (24.00%), 10q22.1 (37. 50%), 11q13-q14 (44.12%), 15q26 (40.63%), 17p13 (25.71%), and 17q21. 3 (37.04%). We defined a 1-cM minimal common deletion in 11q13-q14 between D11S911 and D11S937 markers. A statistical analysis revealed a positive correlation between LOH of 11q13-q14 and clinicopathological data.
Sirchia
S M
SM
Genetica Umana, Dipartimento di Medicina, Chirurgia ed Odontoiatria, Polo San Paolo, Università degli Studi di Milano, Milan, Italy.
Sironi
E
E
Grati
F R
FR
Serafini
P
P
Garagiola
I
I
Rossella
F
F
Dulcetti
F
F
Pardi
G
G
Garsia
S
S
Simoni
G
G
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000328:Adult; D000368:Aged; D002880:Chromosomes, Human, Pair 11; D016889:Endometrial Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D006801:Humans; D019656:Loss of Heterozygosity; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity