title: Microvascular flow and tissue PO(2) in skeletal muscle of chronic reduced renal mass hypertensive rats.
abstract: This study determined whether arteriolar blood flow, capillary red blood cell (RBC) velocity, capillary hematocrit (Hct(cap)), and tissue PO(2) are altered in cremaster muscles of rats with chronic reduced renal mass hypertension (RRM-HT) relative to normotensive rats on high- or low-salt (NT-HS vs. NT-LS) diet. The blood flow in first- through third-order arterioles was not different between NT and HT rats, either at rest or during maximal relaxation of the vessels with 10(-4) M adenosine. Capillary RBC velocity was similar between the groups at rest but was elevated in RRM-HT and NT-HS rats during adenosine superfusion. Hct(cap) was reduced at rest in RRM-HT and NT-HS rats compared with NT-LS and was reduced in RRM-HT rats during adenosine-induced dilation. Tissue PO(2) was reduced in RRM-HT and NT-HS rats compared with NT-LS rats during control conditions and was lower in RRM-HT than in NT-LS rats during adenosine-induced dilation. These results indicate that both RRM-HT and chronic exposure of normotensive rats to a high-salt diet lead to reduced tissue oxygenation, despite the maintenance of normal arteriolar blood flow.
authors.affiliation: Department of Physiology, Medical College of Wisconsin, Milwaukee, 53226, USA. jlombard@mcw.edu
authors.forename: J H
authors.initials: JH
authors.lastname: Lombard
authors.forename: J C
authors.initials: JC
authors.lastname: Frisbee
authors.forename: A S
authors.initials: AS
authors.lastname: Greene
authors.forename: A G
authors.initials: AG
authors.lastname: Hudetz
authors.forename: R J
authors.initials: RJ
authors.lastname: Roman
authors.forename: P J
authors.initials: PJ
authors.lastname: Tonellato
mesh_terms: D000241:Adenosine / Q000494:pharmacology; D000818:Animals; D001160:Arterioles / Q000187:drug effects / Q000378:metabolism*; D001783:Blood Flow Velocity / Q000187:drug effects / Q000502:physiology; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology; D002196:Capillaries / Q000187:drug effects / Q000378:metabolism*; D002908:Chronic Disease; D006400:Hematocrit; D006977:Hypertension, Renal / Q000378:metabolism*; D008297:Male; D018482:Muscle, Skeletal / Q000098:blood supply / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017673:Sodium Chloride, Dietary / Q000494:pharmacology; D014654:Vascular Patency / Q000187:drug effects / Q000502:physiology
chemical_list: D017673:Sodium Chloride, Dietary; D000241:Adenosine; D010100:Oxygen