Ring opening is not rate-limiting in the GTP cyclohydrolase I reaction.
GTP cyclohydrolase I catalyzes a mechanistically complex ring expansion affording dihydroneopterin triphosphate and formate from GTP. Single turnover quenched flow experiments were performed with the recombinant enzyme from Escherichia coli. The consumption of GTP and the formation of 5-formylamino-6-ribosylamino-2-amino-4(3H)-pyrimidinone triphosphate, formate, and dihydroneopterin triphosphate were determined by high pressure liquid chromatography analysis. A kinetic model comprising three consecutive unimolecular steps was used for interpretations where the first intermediate, 5-formylamino-6-ribosylamino-2-amino-4(3H)-pyrimidinone 5'-triphosphate, was formed in a reversible reaction. The rate constant k(1) for the reversible opening of the imidazole ring of GTP was 0.9 s(-1), the rate constant k(3) for the release of formate from 5-formylamino-6-ribosylamino-2-amino-4(3H)-pyrimidinone triphosphate was 2.0 s(-1), and the rate constant k(4) for the formation of dihydroneopterin triphosphate was 0.03 s(-1). Thus, the hydrolytic opening of the imidazole ring of GTP is rapid by comparison with the overall reaction.
Schramek
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N
Lehrstuhl für Organische Chemie und Biochemie, Technische Universität München, Lichtenbergstrasse 4, D-85747 Garching, Germany.
Bracher
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A
Bacher
A
A
D019747:Aldose-Ketose Isomerases / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D017022:Flow Injection Analysis; D005561:Formates / Q000378:metabolism; D006136:GTP Cyclohydrolase / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000378:metabolism*; D008956:Models, Chemical; D019798:Neopterin / Q000031:analogs & derivatives; D011621:Pteridines / Q000378:metabolism; D011742:Pyrimidine Nucleotides / Q000378:metabolism
C119702:2-amino-5-formylamino-6-ribofuranosylamino-4(3H)-pyrimidinone triphosphate; D005561:Formates; D011621:Pteridines; D011742:Pyrimidine Nucleotides; C030544:formic acid; C013490:dihydroneopterin triphosphate; D019798:Neopterin; D006160:Guanosine Triphosphate; D006136:GTP Cyclohydrolase; D019747:Aldose-Ketose Isomerases; C029865:phosphoribosylanthranilate isomerase