title: Epidemiologic application of pulsed-field gel electrophoresis to an outbreak of Campylobacter jejuni in an Austrian youth centre.
abstract: We report the first documented Campylobacter jejuni outbreak in an Austrian youth centre. Sixty-four children were involved of which 38 showed classical signs of campylobacter gastroenteritis. Since unpasteurized milk distributed by a local dairy was suspected to be the source of infection, stool samples were collected from 20 cows providing the milk. Five of the cows tested positive for C. jejuni. These isolates together with 37 clinical samples were compared by pulsed-field-gel electrophoresis (PFGE). The PFGE patterns, using the restriction endonucleases SmaI and SalI, were identical for the human and bovine isolates. This finding confirmed that the outbreak was caused by the consumption of unpasteurized milk contaminated with C. jejuni.
authors.affiliation: Institute of Milk Hygiene, Milk Technology and Food Science, University of Veterinary Medicine, Vienna, Austria.
authors.forename: A
authors.initials: A
authors.lastname: Lehner
authors.forename: C
authors.initials: C
authors.lastname: Schneck
authors.forename: G
authors.initials: G
authors.lastname: Feierl
authors.forename: P
authors.initials: P
authors.lastname: Pless
authors.forename: A
authors.initials: A
authors.lastname: Deutz
authors.forename: E
authors.initials: E
authors.lastname: Brandl
authors.forename: M
authors.initials: M
authors.lastname: Wagner
mesh_terms: D000293:Adolescent; D000818:Animals; D001317:Austria / Q000453:epidemiology; D002169:Campylobacter Infections / Q000453:epidemiology*; D016123:Campylobacter jejuni / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D002648:Child; D002656:Child Day Care Centers; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D005243:Feces / Q000382:microbiology; D005260:Female; D005516:Food Microbiology; D005759:Gastroenteritis / Q000453:epidemiology*; D006801:Humans; D008297:Male; D008892:Milk / Q000382:microbiology*; D011202:Poultry Products / Q000382:microbiology; D011795:Surveys and Questionnaires
chemical_list: D004269:DNA, Bacterial