Potentiation of the effects of bradykinin on its receptor in the isolated guinea pig ileum.
Angiotensin I-converting enzyme (ACE/kininase II) inhibitors potentiated guinea pig ileum's isotonic contractions to bradykinin (BK) and its analogues, shifting the BK dose-response curve to the left. ACE inhibitors added at the peak of the contraction immediately enhanced it further (343 +/- 40%), although the ileum inactivated BK slowly (t(1/2) = 12-16 min). Chymotrypsin and cathepsin G also augmented the activity of BK up to three- or four-fold, but in a manner slower than that of ACE inhibitors. The BK B(2) receptor blocker HOE 140 inhibited all effects. Histamine and angiotensin II were not potentiated. ACE inhibitors potentiate BK independent of blocking its inactivation by inducing crosstalk between ACE and the BK B(2) receptor; proteases activate the receptor by different mechanism.
Minshall
R D
RD
Department of Pharmacology, University of Illinois College of Medicine, Chicago, IL 60612, USA.
Nedumgottil
S J
SJ
Igić
R
R
Erdös
E G
EG
Rabito
S F
SF
D000818:Animals; D001920:Bradykinin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D056649:Cathepsin G; D002403:Cathepsins / Q000494:pharmacology; D002918:Chymotrypsin / Q000037:antagonists & inhibitors / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D015773:Enalaprilat / Q000494:pharmacology; D006168:Guinea Pigs; D006868:Hydrolysis; D007082:Ileum / Q000187:drug effects*; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D011863:Radioimmunoassay; D018002:Receptors, Bradykinin / Q000378:metabolism*; D012697:Serine Endopeptidases
D018002:Receptors, Bradykinin; C065679:icatibant; D002403:Cathepsins; D007703:Peptidyl-Dipeptidase A; D012697:Serine Endopeptidases; D002918:Chymotrypsin; D056649:Cathepsin G; D015773:Enalaprilat; D001920:Bradykinin