title: Rapid combined assay for Salmonella detection in food samples.
abstract: A rapid method was developed to detect salmonellae in food samples. The method gave a possibility to obtain results after 28 h 30 min. The preenrichment in buffered peptone water lasted for 6 h, the enrichment in Rappaport-Vassiliadis medium was applied for 18 h followed by PCR with INVA1-INVA2 primer pair, adapting Chiu and Ou's method. This procedure was suitable to demonstrate salmonella contamination at min. 10 cfu/25 g sample. Out of 18 samples there was a good agreement between the results of the conventional and rapid methods in case of 17 samples. PCR with SPVC1-SPVC2 primer pair informing about the presence of virulence plasmid was performed in separate tubes, because decreased sensitivity was observed in case of multiplex PCR.
authors.affiliation: National Center for Epidemiology B. Johan, Budapest, Hungary.
authors.forename: I
authors.initials: I
authors.lastname: Gadó
authors.forename: P
authors.initials: P
authors.lastname: Major
authors.forename: M
authors.initials: M
authors.lastname: Király
authors.forename: M G
authors.initials: MG
authors.lastname: Pláveczky
mesh_terms: D003470:Culture Media; D005516:Food Microbiology; D008460:Meat / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012475:Salmonella / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D017365:Spices / Q000382:microbiology
chemical_list: D003470:Culture Media