title: An indirect immunofluorescent antibody technique for detection and enumeration of Vibrio vulnificus serovar E (biotype 2): development and applications.
abstract: The applications of an indirect fluorescent antibody technique (IFAT), developed to detect and enumerate the pathogenic bacterium Vibrio vulnificus serovar E from water and clinical samples, are described. This technique proved accurate for detecting V. vulnificus, even under starvation conditions and in the non-culturable state, and could differentiate this species from other bacteria which share the same habitats. The IFAT was successfully used to diagnose vibriosis from naturally- and artificially-infected eels. The overall data suggest that applying this technique properly in environmental and epidemiological/epizootiological studies could significantly increase our knowledge of this bacterium.
authors.affiliation: Department of Microbiología and Ecología, Universidad de Valencia, Valencia, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Marco-Noales
authors.forename: E G
authors.initials: EG
authors.lastname: Biosca
authors.forename: M
authors.initials: M
authors.lastname: Milán
authors.forename: C
authors.initials: C
authors.lastname: Amaro
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D004524:Eels / Q000382:microbiology*; D005393:Fish Diseases / Q000382:microbiology*; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods*; D012623:Seawater / Q000382:microbiology; D012680:Sensitivity and Specificity; D014733:Vibrio / Q000276:immunology / Q000302:isolation & purification*; D014735:Vibrio Infections / Q000382:microbiology / Q000662:veterinary*; D014871:Water Microbiology
chemical_list: D000907:Antibodies, Bacterial