title: The conserved C-terminus of the citrate (CitP) and malate (MleP) transporters of lactic acid bacteria is involved in substrate recognition.
abstract: The membrane potential-generating transporters CitP of Leuconostoc mesenteroides and MleP of Lactococcus lactis are homologous proteins with 48% identical residues that catalyze citrate-lactate and malate-lactate exchange, respectively. The two transporters are highly specific for substrates containing a 2-hydroxycarboxylate motif (HO-CR(2)-COO(-)) in which substitutions of the R groups are tolerated well. Differences in substrate specificity between MleP and CitP are based on subtle changes in the interaction of the protein with the R groups affecting both binding and translocation properties. The conserved, 46-residue long C-terminal region of the transporters containing the C-terminal putative transmembrane segment XI was investigated for its role in substrate recognition by constructing chimeric transporters. Replacement of the C-terminal region of MleP with that of CitP and vice versa did not alter the exchange kinetics with the substrates malate and citrate, indicating that the main interactions between the proteins and di- and tricarboxylate substrates were not altered. In contrast, the interaction of the proteins with the monocarboxylate substrates mandelate and 2-hydroxyisovalerate changed in a complementary manner. The affinity of CitP for the S-enantiomers of these substrates was at least 1 order of magnitude lower than observed for MleP. Introduction of the C-terminal residues of MleP in CitP resulted in a higher affinity and vice versa. Interchanging the C-termini had a more complicated effect on the R-enantiomers, affecting different kinetic parameters with different substrates, indicating multiple interactions of the R groups at this side of the binding pocket. It is suggested that the binding pocket is located between transmembrane segment XI and the other transmembrane segments of the transporters.
authors.affiliation: Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Haren, The Netherlands.
authors.forename: M
authors.initials: M
authors.lastname: Bandell
authors.forename: J S
authors.initials: JS
authors.lastname: Lolkema
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites / Q000235:genetics; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D019343:Citric Acid / Q000378:metabolism; D017124:Conserved Sequence / Q000235:genetics; D019344:Lactic Acid / Q000378:metabolism*; D013294:Lactococcus lactis / Q000235:genetics / Q000378:metabolism; D007934:Leuconostoc / Q000235:genetics / Q000378:metabolism; D008293:Malates / Q000378:metabolism; D008333:Mandelic Acids / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D027361:Organic Anion Transporters; D010446:Peptide Fragments / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000138:chemical synthesis / Q000378:metabolism; D013379:Substrate Specificity / Q000235:genetics; D027981:Symporters
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; C096373:CitP protein, Lactobacillales; D008293:Malates; D008333:Mandelic Acids; D026901:Membrane Transport Proteins; D027361:Organic Anion Transporters; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D027981:Symporters; C098940:malate permease protein, Bacteria; D019343:Citric Acid; D019344:Lactic Acid; C030298:malic acid