Amyloid-like inclusions in Huntington's disease.
Huntington's disease is a progressive, autosomal dominantly inherited, neurodegenerative disease that is characterized by involuntary movements (chorea), cognitive decline and psychiatric manifestations. This is one of a number of late-onset neurodegenerative disorders caused by expanded glutamine repeats, with a likely similar biochemical basis. Immunohistochemical studies on Huntington's disease tissue, using antibodies raised to the N-terminal region of huntingtin (adjacent to the repeat) and ubiquitin, have recently identified neuronal inclusions within densely stained neuronal nuclei, peri-nuclear and within dystrophic neuritic processes. However, the functional significance of inclusions is unknown. It has been suggested that the disease-causing mechanism in Huntington's disease (and the other polyglutamine disorders) is the ability of polyglutamine to undergo a conformational change that can lead to the formation of very stable anti-parallel beta-sheets; more specifically, amyloid structures. We examined, using Congo Red staining and both polarizing and confocal microscopy, post mortem human brain tissue from five Huntington's disease cases, two Alzheimer's disease cases and two normal controls. Brains from five transgenic mice (R6/2)(12) expressing exon 1 of the human huntingtin gene with expanded polyglutamine, and five littermate controls, were also examined by the same techniques. We have shown that some inclusions in Huntington's disease brain tissue possess an amyloid-like structure, suggesting parallels with other amyloid-associated diseases such as Alzheimer's and prion diseases.
McGowan
D P
DP
Department of Anatomy with Radiology, University of Auckland, Private Bag 92019, Symonds Street, Auckland, New Zealand.
van Roon-Mom
W
W
Holloway
H
H
Bates
G P
GP
Mangiarini
L
L
Cooper
G J
GJ
Faull
R L
RL
Snell
R G
RG
D000544:Alzheimer Disease / Q000378:metabolism / Q000473:pathology; D000682:Amyloid / Q000378:metabolism*; D000818:Animals; D001718:Birefringence; D001921:Brain / Q000378:metabolism / Q000473:pathology; D003224:Congo Red; D006801:Humans; D006816:Huntington Disease / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D018613:Microscopy, Confocal; D008859:Microscopy, Polarization; D009474:Neurons / Q000378:metabolism; D013194:Staining and Labeling
D000682:Amyloid; D003224:Congo Red