Neutrophil adhesion and transmigration through bovine endothelial cells in vitro by protein H and LPS of Pasteurella multocida.
This study describes an in vitro investigation on the role of Pasteurella multocida cells and its isolated protein H and LPS on neutrophil adhesion and migration through bovine endothelial cell monolayers. P. multicoda cells, protein H and LPS increased the adhesion and transmigration of neutrophils through BAEC. The bacteria/cell ratio of 100 for P. multocida, protein H concentration 0.05-0.2 microM and LPS concentration 0.5-1.0 microM respectively, induced the maximum adhesion and transmigration of neutrophils through BAEC. The optimal time of incubation with bacteria or bacterial products was 4-6 h. Our results confirm the role of Gram-negative bacteria and of components of the outer membrane such as protein H or LPS in activating the neutrophils and in promoting the adhesion and cells transmigration from the vessels to the site of inflammation.
Galdiero
M
M
Dipartimeno di Patologia e Sanità Animale, Facoltà di Veterinaria, Università degli Studi di Napoli Federico II, Italia. mgaldier@unina.it
Folgore
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Nuzzo
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Galdiero
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D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology* / Q000302:isolation & purification; D002417:Cattle; D002448:Cell Adhesion; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology; D008070:Lipopolysaccharides / Q000276:immunology* / Q000302:isolation & purification; D009504:Neutrophils / Q000276:immunology / Q000502:physiology*; D016979:Pasteurella multocida / Q000276:immunology*; D018272:Porins / Q000276:immunology
D001426:Bacterial Proteins; D008070:Lipopolysaccharides; D018272:Porins