Differential expression of protein kinase C subtypes during ginsenoside Rh2-lnduced apoptosis in SK-N-BE(2) and C6Bu-1 cells.
We examined the modulation of protein kinase C (PKC) subtypes during apoptosis induced by ginsenoside Rh2 (G-Rh2) in human neuroblastoma SK-N-BE(2) and rat glioma C6Bu-1 cells. Apoptosis induced by G-Rh2 in both cell lines was confirmed, as indicated by DNA fragmentation and in situ strand breaks, and characteristic morphological changes. During apoptosis induced by G-Rh2 in SK-N-BE(2) cells, PKC subtypes alpha, beta and gamma were progressively increased with prolonged treatment, whereas PKC delta increased transiently at 3 and 6 h and PKC epsilon was gradually down-regulated after 6 h following the treatment. On the other hand, PKC subtype zeta markedly increased at 24 h when maximal apoptosis was achieved. In C6Bu-1 cells, no significant changes in PKC subtypes alpha, gamma, delta, epsilon and zeta were observed during apoptosis induced by G-Rh2. These results suggest the evidence for a possible role of PKC subtype in apoptosis induced by G-Rh2 in SK-N-BE(2) cells but not in C6Bu-1 cells, and raise the possibility that G-Rh2 may induce apoptosis via different pathways interacting with or without PKC in different cell types.
Kim
Y S
YS
Korea Ginseng and Tobacco Research Institute, Youseong-Gu, Taejon. yskim@gtr.kgtri.re.kr
Jin
S H
SH
Lee
Y H
YH
Park
J D
JD
Kim
S I
SI
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D036145:Ginsenosides; D006801:Humans; D007527:Isoenzymes / Q000502:physiology*; D011493:Protein Kinase C / Q000502:physiology*; D051381:Rats; D012503:Saponins / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; D007527:Isoenzymes; D012503:Saponins; C055305:ginsenoside Rh2; D011493:Protein Kinase C