Dexamethasone increases the incorporation of [3H]serine into phosphatidylserine and the activity of serine base exchange enzyme in mouse thymocytes: a possible relation between serine base exchange enzyme and apoptosis.
The exposure of phosphatidylserine toward the external surface of the membrane is a well-established event of programmed cell death. The possibility that an apoptotic stimulus influences the metabolism of this phospholipid could be relevant not only in relation to the previously mentioned event but also in relation to the capability of membrane phosphatidylserine to influence PKC activity. The present investigation demonstrates that treatment of mouse thymocytes with the apoptotic stimulus dexamethasone, enhances the incorporation of [3H]serine into phosphatidylserine. Cell treatment with dexamethasone also enhanced the activity of serine base exchange enzyme, assayed in thymocyte lysate. Both the effects were observed at periods of treatment preceding DNA fragmentation. The addition of unlabelled ethanolamine, together with [3H]serine to the medium containing dexamethasone-treated thymocytes lowered the radioactivity into phosphatidylserine. Serine base exchange enzyme activity was influenced by the procedure used to prepare thymocyte lysate and was lowered by the addition of fluoroaluminate, that is widely used as a G-protein activator. The increase of serine base exchange enzyme activity induced by dexamethasone treatment was observed independently by the procedure used to prepare cell lysate and by the presence or absence of fluoroaluminate.
Buratta
S
S
Department of Internal Medicine and Endocrine and Metabolic Sciences-Biochemistry Laboratory, University of Perugia, Italy.
Migliorati
G
G
Marchetti
C
C
Mambrini
R
R
Riccardi
C
C
Mozzi
R
R
D017607:Aluminum Compounds / Q000494:pharmacology; D000818:Animals; D017209:Apoptosis; D002457:Cell Extracts; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology*; D005434:Flow Cytometry; D005459:Fluorides / Q000494:pharmacology; D051379:Mice; D019884:Nitrogenous Group Transferases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010718:Phosphatidylserines / Q000378:metabolism*; D012694:Serine / Q000378:metabolism*; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D014316:Tritium / Q000378:metabolism*
D017607:Aluminum Compounds; D002457:Cell Extracts; D010718:Phosphatidylserines; D014316:Tritium; C050992:tetrafluoroaluminate; D012694:Serine; D003907:Dexamethasone; D003513:Cycloheximide; D019884:Nitrogenous Group Transferases; D005459:Fluorides