title: Argadin, a new chitinase inhibitor, produced by Clonostachys sp. FO-7314.
abstract: A new chitinase inhibitor, designated as argadin (1), was isolated from the cultured broth of a fungal strain FO-7314. The strain was identified as Clonostachys sp. from the morphological characteristics. Argadin was purified from the cultured mycelium by a combination of cation exchange, adsorption and gel filtration chromatographic methods. The structure of argadin was elucidated as cyclo(Nomega-acetyl-L-arginyl-D-prolyl-homoseryl-histidyl-L- 2-aminoadipyl) in which homoseryl gamma-methylene bonded to histidyl alpha-amino residue. The IC50 value of argadin against Lucilia cuprina (blowfly) chitinase was 150 nM at 37 degrees C and 3.4 nM at 20 degrees C. Argadin arrested the moult of cockroach larvae upon injection into the ventral abdominal part.
authors.affiliation: Research Center for Biological Function, The Kitasato Institute, Tokyo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Arai
authors.forename: K
authors.initials: K
authors.lastname: Shiomi
authors.forename: Y
authors.initials: Y
authors.lastname: Yamaguchi
authors.forename: R
authors.initials: R
authors.lastname: Masuma
authors.forename: Y
authors.initials: Y
authors.lastname: Iwai
authors.forename: A
authors.initials: A
authors.lastname: Turberg
authors.forename: H
authors.initials: H
authors.lastname: Kölbl
authors.forename: S
authors.initials: S
authors.lastname: Omura
mesh_terms: D000818:Animals; D002688:Chitinases / Q000037:antagonists & inhibitors*; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification*; D008854:Microscopy, Electron; D003904:Mitosporic Fungi / Q000737:chemistry* / Q000648:ultrastructure; D015394:Molecular Structure; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification*; D013057:Spectrum Analysis
chemical_list: D004791:Enzyme Inhibitors; D010456:Peptides, Cyclic; C419444:argadin; D002688:Chitinases