title: Primary habitual abortions are associated with high frequency of factor V Leiden mutation.
abstract: OBJECTIVE
  To analyze the prevalence of the mutation G1691A in factor V gene (Leiden mutation), of mutation C677T in the methylenetetrahydrofolate reductase (MTHFR) gene, and of polymorphism in G20210A in the prothrombin gene in women with recurrent abortions; further, to identify a subgroup at higher risk of being carriers of these mutations.
  
  
  METHODS
  Prospective case control evaluation.
  
  
  METHODS
  University clinic.
  
  
  METHODS
  Eighty-four women with 3 or more consecutive miscarriages were compared with 69 controls.
  
  
  METHODS
  None.
  
  
  METHODS
  Polymerase chain reactions were performed to identify the mutations G1691A in factor V and C677T in MTHFR genes and the polymorphism G20210A in the prothrombin gene.
  
  
  RESULTS
  In women with primary habitual abortions, 27.8% carried the Leiden mutation. No difference was observed in the prevalence of mutation C677T in the MTHFR gene or in polymorphism G20210A in the prothrombin gene.
  
  
  CONCLUSIONS
  The Leiden mutation may play a considerable role for women having primary recurrent abortions.
authors.affiliation: Department of Woman and Child Health, Karolinska Institute, Stockholm, Sweden. margaretha.wramsby@kbh.ki.se
authors.forename: M L
authors.initials: ML
authors.lastname: Wramsby
authors.forename: M
authors.initials: M
authors.lastname: Sten-Linder
authors.forename: K
authors.initials: K
authors.lastname: Bremme
mesh_terms: D000026:Abortion, Habitual / Q000235:genetics*; D000328:Adult; D016022:Case-Control Studies; D005165:Factor V / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D006801:Humans; D042965:Methylenetetrahydrofolate Reductase (NADPH2); D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000235:genetics; D011110:Polymorphism, Genetic; D011446:Prospective Studies; D011516:Prothrombin / Q000235:genetics
chemical_list: C095381:factor V Leiden; D005165:Factor V; D011516:Prothrombin; D000587:Oxidoreductases Acting on CH-NH Group Donors; D042965:Methylenetetrahydrofolate Reductase (NADPH2)