title: The immunogenicity and efficacy of replication-defective and replication-competent bovine adenovirus-3 expressing bovine herpesvirus-1 glycoprotein gD in cattle.
abstract: Replication-competent and replication-defective bovine adenovirus type 3 recombinants expressing the bovine herpesvirus type 1 (BHV-1) glycoprotein D (gD) were tested for induction of gD specific immune responses in calves using intratracheal (1st and 2nd immunization) and sub-cutaneous (3rd immunization) route of immunization. The replication-defective recombinant BAV501 induced systemic immune responses against gD as low titers of anti gD-IgG were detected in the serum. However, the efficacy of the replication-competent BAV3.E3gD to induce gD-specific antibodies in the serum and the nasal secretions was superior to that of replication-defective BAV501 when both viruses were given at the same dosage. Partial protection from challenge was induced in calves immunized with replication-competent BAV3.E3gD. A dramatic increase in the titers of anti-gD IgG and IgA levels, both in serum and nasal secretions, following BHV-1 challenge (anamnestic response) suggested that the animals immunized with replication-defective BAV501 had been primed for gD-specific antibody responses.
authors.affiliation: Veterinary Infectious Disease Organization, University of Saskatchewan, Saskatoon, Sask., Canada S7N 5E3.
authors.forename: P S
authors.initials: PS
authors.lastname: Reddy
authors.forename: N
authors.initials: N
authors.lastname: Idamakanti
authors.forename: C
authors.initials: C
authors.lastname: Pyne
authors.forename: A N
authors.initials: AN
authors.lastname: Zakhartchouk
authors.forename: D L
authors.initials: DL
authors.lastname: Godson
authors.forename: Z
authors.initials: Z
authors.lastname: Papp
authors.forename: M E
authors.initials: ME
authors.lastname: Baca-Estrada
authors.forename: L A
authors.initials: LA
authors.lastname: Babiuk
authors.forename: G K
authors.initials: GK
authors.lastname: Mutwiri
authors.forename: S K
authors.initials: SK
authors.lastname: Tikoo
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000276:immunology; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000097:blood; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005822:Genetic Vectors / Q000276:immunology; D006566:Herpesviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D007242:Herpesvirus 1, Bovine / Q000235:genetics / Q000276:immunology*; D009500:Neutralization Tests; D014611:Vaccination / Q000662:veterinary*; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology* / Q000592:standards
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D014614:Vaccines, Synthetic; D014764:Viral Proteins; D014765:Viral Vaccines; C046998:bovine herpesvirus type-1 glycoproteins