title: Scleral remodeling during the development of and recovery from axial myopia in the tree shrew.
abstract: OBJECTIVE
  Recent investigations have suggested that scleral thinning in mammalian eyes with axial myopia is a consequence of the loss of scleral tissue, rather than the redistribution of existing tissue as the eye enlarges. The present study investigated whether further changes in the distribution and metabolism of scleral tissue occur during the process of recovery from axial myopia. Scleral glycosaminoglycan (GAG) synthesis and content as well as scleral dry weight changes were monitored as indicators of remodeling in myopic and recovering tree shrew sclerae.
  
  
  METHODS
  Myopia was induced in tree shrews by monocularly depriving them of pattern vision. Some animals then had the occluder removed and were allowed to recover from the induced myopia for periods of 1, 3, 5, 7, and 9 days. Newly synthesized GAGs were radiolabeled in vivo with [(35)S]sulfate. Sulfate incorporation and total GAG content in the sclera was measured through selective precipitation of GAGs from proteinase K digests with alcian blue dye. Dry weights of the sclerae were also determined. Changes in ocular refraction and eye size were monitored using retinoscopy, keratometry, and ultrasonography.
  
  
  RESULTS
  Eyes developing myopia showed a significant reduction in scleral GAG synthesis, particularly in the region of the posterior pole (-36% +/- 7%) compared with contralateral control eyes. Scleral dry weight was also significantly reduced in these eyes (-3.7% +/- 1.2%). In recovering eyes, significant changes in GAG synthesis were apparent after 24 hours of recovery. After 3 days of recovery, significantly elevated levels of GAG synthesis were found (+79% +/- 15%), returning to contralateral control eye values after 9 days of recovery. Interocular differences in scleral dry weight were shown to follow a similar pattern to that observed for GAG synthesis.
  
  
  CONCLUSIONS
  Active remodeling, resulting in either the loss or replacement of scleral tissue and not passive redistribution of scleral tissue, is associated with changes in eye size during both myopia development and recovery. Regulatory changes in scleral metabolism can be rapidly evoked by a change in visual conditions and the direction of regulation is related to the direction of change in eye size.
authors.affiliation: Department of Optometry and Vision Sciences, The University of Melbourne, Victoria, Australia. n.mcbrien@optometry.unimelb.edu.au
authors.forename: N A
authors.initials: NA
authors.lastname: McBrien
authors.forename: P
authors.initials: P
authors.lastname: Lawlor
authors.forename: A
authors.initials: A
authors.lastname: Gentle
mesh_terms: D000818:Animals; D003094:Collagen / Q000378:metabolism; D004247:DNA / Q000032:analysis; D006025:Glycosaminoglycans / Q000378:metabolism; D009216:Myopia / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology*; D009929:Organ Size; D012029:Refraction, Ocular; D012590:Sclera / Q000378:metabolism / Q000503:physiopathology*; D012683:Sensory Deprivation; D014418:Tupaiidae
chemical_list: D006025:Glycosaminoglycans; D003094:Collagen; D004247:DNA