Renal nerve inhibition by central NaCl and ANG II is abolished by lesions of the lamina terminalis.
The lamina terminalis is situated in the anterior wall of the third ventricle and plays a major role in fluid and electrolyte homeostasis and cardiovascular regulation. The present study examined whether the effects of intracerebroventricular infusion of hypertonic saline and ANG II on renal sympathetic nerve activity (RSNA) were mediated by the lamina terminalis. In control, conscious sheep (n = 5), intracerebroventricular infusions of 0.6 M NaCl (1 ml/h for 20 min) and ANG II (10 nmol/h for 30 min) increased mean arterial pressure (MAP) by 6 +/- 1 (P < 0.001) and 14 +/- 3 mmHg (P < 0.001) and inhibited RSNA by 80 +/- 6 (P < 0.001) and 89 +/- 7% (P < 0.001), respectively. Both treatments reduced plasma renin concentration (PRC). Intracerebroventricular infusion of artificial cerebrospinal fluid (1 ml/h for 30 min) had no effect. In conscious sheep with lesions of the lamina terminalis (n = 6), all of the responses to intracerebroventricular hypertonic saline and ANG II were abolished. In conclusion, the effects of intracerebroventricular hypertonic saline and ANG II on RSNA, PRC, and MAP depend on the integrity of the lamina terminalis, indicating that this site plays an essential role in coordinating the homeostatic responses to changes in brain Na(+) concentration.
May
C N
CN
Howard Florey Institute of Physiology and Medicine, University of Melbourne, Parkville 3052, Australia. c.may@hfi.unimelb.edu.au
McAllen
R M
RM
McKinley
M J
MJ
D000804:Angiotensin II / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000187:drug effects*; D002555:Cerebrospinal Fluid; D005260:Female; D007031:Hypothalamus / Q000033:anatomy & histology / Q000502:physiology* / Q000601:surgery; D007668:Kidney / Q000294:innervation*; D012083:Renin / Q000097:blood; D012462:Saline Solution, Hypertonic / Q000494:pharmacology; D012756:Sheep; D012965:Sodium Chloride / Q000008:administration & dosage / Q000494:pharmacology*; D013564:Sympathetic Nervous System / Q000502:physiology
D012462:Saline Solution, Hypertonic; D000804:Angiotensin II; D012965:Sodium Chloride; D012083:Renin