[Studies of development of disomic addition lines of Triticum aestivum-Haynaldia villosa via AABBDDDD octaploid].
Hexaploid hybrid between Triticum aestivum-Aegilops squarrosa (2n = 8x = 56, AABBDDDD) octaploid and Triticum durum-Haynaldia villosa (2n = 6x = 42, AABBVV) was self pollinated. The putitative T. aestivum-H. villosa 6V and 2V addition lines were creened by C-banding in F4 and chromosome configurations of PMC at MI were 0.14I + 20.42II + 1.50II and 0.10 I + 20.07II + 1.82II, respectively. Genomic DNA of 95-7 and 26-7 were digested by EcoRI, and Southern bloting was employed using group 6 probe Psr113 for 95-7 and group 2 probe BCD240 fro 26-7. The results showed that 95-7 and 26-7 had the same special bands as H. villosa did. Therefore, 95-7 and 26-7 further identified were T. aestivum-H. villosa 6V and 2V disomic addition lines.
Ying
J
J
Key Laboratory of Crop Cytogenetics, Nanjing Agricultural University, China.
Chen
P D
PD
D003433:Crosses, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011123:Polyploidy; D014908:Triticum / Q000235:genetics*