Effect of polyclonal anti-TNFalpha antibody on endotoxic shock in suckling rats.
The aim of this study was to evaluate the effect of anti-tumor necrosis factor alpha (TNFalpha) antibodies on the TNFalpha gene expression in a neonatal septic shock model. Ten-day-old Sprague-Dawley rats were divided into four groups and given intraperitoneal (ip) injection as follows: group 1: 0.1 ml saline; group 2: 0.1 mg/kg Salmonella enteritidis lipopolysaccharide (LPS); group 3: 1 mg/kg of anti-TNFalpha antibodies (Ab); group 4: 0.1 mg/kg of LPS and 1 mg/kg of Ab. We found that in group 2, LPS induced shock, demonstrating hypoglycemia and lactacidemia (p < 0. 05) and death (81.8%). Ab decreased the mortality significantly (35%) and attenuated the hypoglycemia (35 +/- 8 mg/dl in group 2 vs. 53 +/- 3 mg/dl in group 4) and lactacidemia (5.40 +/- 0.63 vs. 2.35 +/- 0.45 mmol) at 8 h in group 4 when compared to group 2. Northern blot demonstrated a significant decrease in TNFalpha mRNA expression in group 4 as compared to group 2, at 2 h after LPS injection. We conclude that the beneficial effects of anti-TNFalpha antibodies on LPS-induced shock may be due to decreased TNFalpha gene expression.
Bhola
M
M
The Ronald McDonald Children's Hospital, Loyola University of Chicago, Maywood, IL 60153, USA. mbhola@luc.edu
Goto
M
M
Chen
H Y
HY
Myers
T F
TF
D000818:Animals; D000833:Animals, Suckling / Q000097:blood*; D000906:Antibodies / Q000494:pharmacology*; D001786:Blood Glucose / Q000032:analysis; D007773:Lactates / Q000097:blood; D012333:RNA, Messenger / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D012772:Shock, Septic / Q000097:blood*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology*
D000906:Antibodies; D001786:Blood Glucose; D007773:Lactates; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha