Spectroscopic imaging of glutamate C4 turnover in human brain.
One-dimensional spectroscopic imaging of (13)C-4-glutamate turnover is performed in the human brain with a 6 cc nominal voxel resolution at 4T. Data were acquired with an indirect detection approach using a short spin echo single quantum (1)H-(13)C heteronuclear editing method and a 7 cm surface coil with quadrature (13)C decoupling coils. To analyze the data as a function of tissue type, T(1)-based image segmentation through the surface coil was performed to determine the gray and white matter contributions to each voxel. The tricarboxylic acid (TCA) cycle rate in gray and white matter was then determined using a two-compartment model with the tissue fractionation derived from the image segmentation. The mean values for the TCA cycle rate for occipital gray and white matter from three volunteers was 0.88 +/- 0.12 and 0.28 +/- 0.13 respectively, in agreement with literature data.
Pan
J W
JW
Department of Medicine, Brookhaven National Laboratory, Upton, New York 11973-5000, USA. jpan@bnl.gov
Stein
D T
DT
Telang
F
F
Lee
J H
JH
Shen
J
J
Brown
P
P
Cline
G
G
Mason
G F
GF
Shulman
G I
GI
Rothman
D L
DL
Hetherington
H P
HP
D001921:Brain / Q000378:metabolism*; D002247:Carbon Isotopes; D002952:Citric Acid Cycle / Q000502:physiology; D018698:Glutamic Acid / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*
D002247:Carbon Isotopes; D018698:Glutamic Acid