title: Anti-CD2 monoclonal antibodies prevent the induction of experimental autoimmune myocarditis.
abstract: We investigated the effect of a monoclonal antibody against CD2 molecules (OX34) in preventing the induction of experimental autoimmune myocarditis (EAM) induced by immunizing Lewis rats with cardiac myosin. Administration of OX34 before immunization, on Days -6, -4, -2 and 0, completely prevented EAM. On the other hand, treatment with OX34 just before the appearance of myocardial lesions, on Days 9, 11, 13 and 15, had only a partial effect in preventing the disease. Flow cytometric analysis of lymph node cells showed that CD3+ T cells were immediately depleted with the administration of OX34 but had largely recovered on Day 21. Lymph node cells in OX34-treated rats had no proliferative responses to cardiac myosin-rod, but the proliferation was restored when recombinant IL-2 was added. Ultimate production of the anti-myosin antibody was not inhibited by the treatment with OX34. These results suggest that the prevention of EAM by administering the anti-CD2 monoclonal antibody OX34 resulted from T cell depletion during the induction phase, and might in addition result from T cell anergy of Th1, but not Th2 cells.
authors.affiliation: Department of Internal Medicine & Cardiology, Kitasato University School of Medicine, Kanagawa, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Inomata
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
authors.forename: M
authors.initials: M
authors.lastname: Haga
authors.forename: H
authors.initials: H
authors.lastname: Hirahara
authors.forename: T
authors.initials: T
authors.lastname: Abo
authors.forename: Y
authors.initials: Y
authors.lastname: Okura
authors.forename: H
authors.initials: H
authors.lastname: Hanawa
authors.forename: M
authors.initials: M
authors.lastname: Kodama
authors.forename: T
authors.initials: T
authors.lastname: Izumi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology / Q000627:therapeutic use*; D001327:Autoimmune Diseases / Q000276:immunology / Q000517:prevention & control*; D017252:CD3 Complex / Q000276:immunology; D015704:CD4 Antigens / Q000276:immunology; D016827:CD8 Antigens / Q000276:immunology; D009205:Myocarditis / Q000276:immunology / Q000517:prevention & control*; D009218:Myosins / Q000276:immunology; D051381:Rats; D011917:Rats, Inbred Lew; D013601:T-Lymphocytes / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens; C112333:OX-34 monoclonal antibody; D009218:Myosins