Antimutagenic activity of 5alpha-cholest-7-en-3beta-ol, a new component from the starfish asterina pectinifera.
From the butanol fraction of the starfish Asterina pectinifera Müler et Troschel (Asteriidae), we have isolated a new component, 5alpha-cholest-7-en-3beta-ol. Its antigenotoxic and antimutagenic activities were examined by the SOS chromotest with Escherichia coli PQ37 and by Ames test with Salmonella typhimurium TA1538, respectively. 5alpha-Cholest-7-en-3beta-ol showed potent antigenotoxic activity against the mutagens, both MNNG and NQO. For 100% of antigenotoxicity, the concentration of the compound applied against MNNG and NQO were 10 microg and 5 microg per reaction tube, respectively. Its antimutagenic activity with S. typhimurium TA1538 against the mutagen MNNG was very effective. When its concentrations were varied from 1 up to 10 microg dose per plate, the inhibition ratio of revertant CFU of TA1538 per plate was increased accordingly, from 25.2 to 99.2%. These results suggest that 5alpha-cholest-7-en-3beta-ol possesses antigenotoxic and antimutagenic activity and might be useful as a chemopreventive agent.
Han
Y H
YH
Department of Biology, Dongguk University, Kyongju, Kyongbuk, Republic of Korea.
Ham
J H
JH
Lee
N J
NJ
Park
C H
CH
Shin
Y H
YH
Lee
D U
DU
D015112:4-Nitroquinoline-1-oxide / Q000633:toxicity; D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D002784:Cholesterol / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D008769:Methylnitronitrosoguanidine / Q000633:toxicity; D015363:Quinolones / Q000633:toxicity; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D013215:Starfish / Q000737:chemistry*; D014020:Tissue Extracts / Q000494:pharmacology
C120195:4-nitroquinolone-1-oxide; D016587:Antimutagenic Agents; D015363:Quinolones; D014020:Tissue Extracts; D008769:Methylnitronitrosoguanidine; D015112:4-Nitroquinoline-1-oxide; C001521:lathosterol; D002784:Cholesterol