title: Phylogenetic relation of lungfish indicated by the amino acid sequence of myelin DM20.
abstract: The cDNA of lungfish Protopterus annectens myelin DM20 was cloned, and the complete amino acid sequence of Protopterusannectens DM20 was deduced. When five possible phylogenetic trees were tested for the DM20 sequences, the maximum likelihood method supported tree 1 [((tetrapods, lungfish), coelacanth), zebrafish, shark] or tree 5 [(tetrapods, lungfish), (coelacanth, zebrafish), shark]. Both tree 1 and tree 5 indicate that lungfish is phylogenetically the closest to tetrapods among the living fishes.
authors.affiliation: Institute of Life Science, Soka University, Hachioji, 192-8577, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Tohyama
authors.forename: T
authors.initials: T
authors.lastname: Ichimiya
authors.forename: H
authors.initials: H
authors.lastname: Kasama-Yoshida
authors.forename: Y
authors.initials: Y
authors.lastname: Cao
authors.forename: M
authors.initials: M
authors.lastname: Hasegawa
authors.forename: H
authors.initials: H
authors.lastname: Kojima
authors.forename: Y
authors.initials: Y
authors.lastname: Tamai
authors.forename: T
authors.initials: T
authors.lastname: Kurihara
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001923:Brain Chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D019143:Evolution, Molecular; D005399:Fishes / Q000235:genetics*; D008969:Molecular Sequence Data; D018991:Myelin Proteolipid Protein / Q000235:genetics*; D009419:Nerve Tissue Proteins; D010802:Phylogeny; D012333:RNA, Messenger / Q000032:analysis; D029961:Zebrafish Proteins
chemical_list: D018076:DNA, Complementary; D018991:Myelin Proteolipid Protein; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D029961:Zebrafish Proteins; C489074:plp1a protein, zebrafish; C489073:plp1b protein, zebrafish