Quantitative gene expression analysis in microdissected archival tissue by real-time RT-PCR.
Laser-assisted microdissection is a powerful tool for the analysis of morphologically defined cell populations. We report here that the combination of microdissection and real-time RT-PCR technologies together with an optimized RNA microscale extraction procedure allows the reproducible determination of gene expression levels in archival formalin-fixed, paraffin-embedded tissue samples.
Specht
K
K
GSF-Forschungszentrum für Umwelt und Gesundheit, Institut für Pathologie, Neuherberg, Germany.
Richter
T
T
Müller
U
U
Walch
A
A
Höfler
M W
MW
D004210:Dissection / Q000379:methods*; D015870:Gene Expression; D006652:Histological Techniques; D006801:Humans; D007834:Lasers; D009369:Neoplasms / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012313:RNA; D018719:Erb-b2 Receptor Tyrosine Kinases