Replication of wild-type and mutant human cytomegalovirus in life-extended human diploid fibroblasts.
A cDNA encoding the catalytic subunit of human telomerase was used to generate life-extended derivatives of primary human diploid fibroblasts. The life-extended cells supported efficient human cytomegalovirus (HCMV) replication. A subclone of the life-extended cells was generated containing the HCMV UL82 gene and used to isolate and propagate a virus that exhibited a profound growth defect after infection at a low input multiplicity.
Bresnahan
W A
WA
Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544-1014, USA. wbresnahan@molbio.princeton.edu
Hultman
G E
GE
Shenk
T
T
D003587:Cytomegalovirus / Q000235:genetics* / Q000502:physiology*; D005347:Fibroblasts / Q000502:physiology* / Q000821:virology*; D006801:Humans; D009154:Mutation; D019098:Telomerase / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D014779:Virus Replication
D014764:Viral Proteins; C051298:cytomegalovirus phosphoprotein 71kDa; D019098:Telomerase