[Effects of spinal cord extract on neurite growth of GABAergic and dynergic neurons of spinal cord in vitro].
We use the crude extract from human fetal spinal cord to study the effect of endogenous substances of the spinal cord on neurite growth of E 12-15 mouse GABAergic and DNYergic neurons of the spinal cord in vitro. The results indicated that the crude extract had no effect on GABAergic neurons but had markedly neurite-promoting effect on DNYergic neurons when the protein concentration of the crude extract was 250 micrograms/ml. It suggests that there are endogenous substances in the crude extract of the human fetal spinal cord, which can promote neurite growth of spinal cord neurons and this effect is neuron type specific at particular embryonic age.
Liang
Z
Z
Fourth Military Medical University, Neuroscience Research Institute of PLA, Xian.
Nong
Y
Y
Yang
H
H
Wang
H J
HJ
Bao
X
X
Ju
G
G
D000818:Animals; D018929:Cell Culture Techniques; D004399:Dynorphins / Q000378:metabolism*; D004622:Embryo, Mammalian; D005333:Fetus; D006801:Humans; D051379:Mice; D009474:Neurons / Q000187:drug effects; D013116:Spinal Cord / Q000737:chemistry* / Q000166:cytology; D014020:Tissue Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D014020:Tissue Extracts; D005680:gamma-Aminobutyric Acid; D004399:Dynorphins