Liquid chromatographic determination of nicarbazin in feeds.
A new liquid chromatographic method has been developed for determination of nicarbazin in feeds. Approximately 40 g feed is extracted with 200 mL acetonitrile-water (80 + 20, v/v). An aliquot of the extract is filtered and assayed using a reversed-phase isocratic method that measures the 4,4'-dinitrocarbanilide moiety of nicarbazin at a wavelength of 340 nm. For medicated feeds, the method uses a standard linear range of 5 to 100 microg/mL. For lower levels, a linear range of 50 to 150 ng/mL can be used. The method has a limit of detection of 250 ng/g and a limit of quantitation of 500 ng/g in a 40 g feed sample. Recovery was 99.1%, with a range of 95.2 to 101.8%. In the typical U.S. dosing range of 27 to 113.5 g/ton, the precision of the method based on one analyst, one day, and 2 weighings ranged from 2.8% (113.5 g/ton) to 4.7% (27 g/ton).
Krabel
B J
BJ
Elanco Animal Health, A Division of Eli Lilly and Company, Greenfield, IN 46140-0708, USA.
Dickson
D A
DA
Zimmermann
A G
AG
Coleman
M R
MR
D000821:Animal Feed / Q000032:analysis*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D003049:Coccidiostats / Q000032:analysis*; D007202:Indicators and Reagents; D009528:Nicarbazin / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results
D003049:Coccidiostats; D007202:Indicators and Reagents; D009528:Nicarbazin