title: Association of protease activity in Vibrio cholerae vaccine strains with decreases in transcellular epithelial resistance of polarized T84 intestinal epithelial cells.
abstract: Culture supernatants prepared from reactogenic strains of Vibrio cholerae cause a decrease in the transcellular epithelial resistance of T84 intestinal cells. This decrease correlates with the presence of hemagglutinin/protease but not with the presence of other potential accessory toxins or proteases. These data suggest a possible role for hemagglutinin/protease in reactogenicity, although other factors may also contribute.
authors.affiliation: Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: S F
authors.initials: SF
authors.lastname: Mel
authors.forename: K J
authors.initials: KJ
authors.lastname: Fullner
authors.forename: S
authors.initials: S
authors.lastname: Wimer-Mackin
authors.forename: W I
authors.initials: WI
authors.lastname: Lencer
authors.forename: J J
authors.initials: JJ
authors.lastname: Mekalanos
mesh_terms: D000626:Aminopeptidases / Q000502:physiology; D002460:Cell Line; D016764:Cell Polarity; D002772:Cholera Toxin / Q000096:biosynthesis; D022121:Cholera Vaccines; D010450:Endopeptidases / Q000502:physiology*; D004731:Endotoxins; D006388:Hemagglutinins / Q000502:physiology; D007413:Intestinal Mucosa / Q000502:physiology*; D014734:Vibrio cholerae / Q000502:physiology*
chemical_list: D022121:Cholera Vaccines; D004731:Endotoxins; D006388:Hemagglutinins; C077728:zonula occludens toxin, Vibrio cholerae; D002772:Cholera Toxin; D010450:Endopeptidases; D000626:Aminopeptidases