Modulation by soluble factors of gelatinase activities released by osteoblastic cells.
This study investigated the ability of normal human osteoblasts (hOb) and osteogenic sarcoma cells (MG-63 and SaOS2) to produce gelatinases and undergo modulation by interleukin 1beta (IL-1beta), interleukin 6 (IL-6), oncostatin M (OSM), leukaemia inhibitory factor (LIF), growth hormone (GH) and insulin-like growth factor-I (IGF-I). Gelatinase activities were determined by zymogaphy, and a quantitative analysis was performed by ELISA. The MMP-2 activities of the three cell lines were significantly increased in the presence of IL-1beta and IL-6, but no modulation of MMP-2 activities was observed in the presence of OSM, LIF and GH. IGF-I increased the activity released by SaOS2 and hOb, but no modulation was detectable in MG-63 cell conditioned medium. An upmodulation of pro-MMP-2 secretion by SaOS2 and hOb was observed for all soluble factors used, whereas an upmodulation of pro-MMP-2 secretion by MG-63 was observed only in the presence of IL-1beta, IL-6 and IGF-I. Thus, osteoblastic cells modulated by cytokines can be involved in bone resorption as a result of the protease activities released.
Damiens
C
C
Laboratoire de Physiopathologie de la Résorption Osseuse, EE 99-01, Faculté de Médecine, 1 rue Gaston Veil, Nantes, 44035, France.
Fortun
Y
Y
Charrier
C
C
Heymann
D
D
Padrines
M
M
D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D018093:Gelatinases / Q000378:metabolism*; D013006:Growth Hormone / Q000378:metabolism; D006131:Growth Inhibitors / Q000378:metabolism; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D007375:Interleukin-1 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D053683:Oncostatin M; D010006:Osteoblasts / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D012509:Sarcoma / Q000378:metabolism; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
D006131:Growth Inhibitors; D007375:Interleukin-1; D015850:Interleukin-6; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines; C508351:OSM protein, human; D010455:Peptides; D053683:Oncostatin M; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone; D018093:Gelatinases; D020778:Matrix Metalloproteinase 2