Structural analysis of N-glycans from yellow lupin (Lupinus luteus) seed diphosphonucleotide phosphatase/phosphodiesterase.
N-linked oligosaccharide chains released by hydrazinolysis from yellow lupin seed diphosphonucleotide phosphatase/phosphodiesterase were fluorescence labeled and separated by high performance liquid chromatography (GlycoSep N and GlycoSep H columns). Exoglycosidase sequencing elucidated the structures of 24 separated N-glycans. Thirty percent of isolated glycans were found to be of high-mannose type (three to eight mannosyl residues), 42% were complex type and 26% belonged to paucimannosidic type. Among complex type glycans, structures with Lewis(a) epitope were identified. It is very unusual to find all types of plant N-glycans in one protein. Possible reasons for such a broad spectrum of N-glycan structures are discussed.
Olczak
M
M
Institute of Biochemistry and Molecular Biology, Wroclaw University, Tamka 2, 50-137, Wroclaw, Poland.
Watorek
W
W
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D007887:Fabaceae / Q000201:enzymology*; D006026:Glycoside Hydrolases; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D010946:Plants, Medicinal; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism*; D011755:Pyrophosphatases / Q000378:metabolism*; D012639:Seeds / Q000201:enzymology*
D009844:Oligosaccharides; D011134:Polysaccharides; D010727:Phosphoric Diester Hydrolases; D006026:Glycoside Hydrolases; D011755:Pyrophosphatases; C405782:nucleotide pyrophosphatase - phosphodiesterase I