Efficacy and safety studies of a recombinant chimeric respiratory syncytial virus FG glycoprotein vaccine in cotton rats.
Several formulations of a recombinant chimeric respiratory syncytial virus (RSV) vaccine consisting of the extramembrane domains of the F and G glycoproteins (FG) were tested in cotton rats to evaluate efficacy and safety. The FG vaccine was highly immunogenic, providing nearly complete resistance to pulmonary infection at doses as low as 25 ng in spite of inducing relatively low levels of serum neutralizing antibody at low vaccine doses. Upon RSV challenge animals primed with FG vaccine showed quite mild alveolitis and interstitial pneumonitis, which were eliminated by the addition of monophosphoryl lipid A to the formulation.
Prince
G A
GA
Virion Systems, Inc., Rockville, Maryland 20850, USA. gprince@erols.com
Capiau
C
C
Deschamps
M
M
Fabry
L
L
Garçon
N
N
Gheysen
D
D
Prieels
J P
JP
Thiry
G
G
Van Opstal
O
O
Porter
D D
DD
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D008168:Lung / Q000473:pathology / Q000821:virology; D017563:Lung Diseases, Interstitial / Q000517:prevention & control; D009500:Neutralization Tests; D011024:Pneumonia, Viral / Q000517:prevention & control; D011993:Recombinant Fusion Proteins / Q000276:immunology; D018357:Respiratory Syncytial Virus Infections / Q000517:prevention & control*; D022261:Respiratory Syncytial Virus Vaccines / Q000008:administration & dosage / Q000276:immunology; D012136:Respiratory Syncytial Viruses / Q000276:immunology* / Q000302:isolation & purification; D016205:Sigmodontinae; D014611:Vaccination; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*
D000914:Antibodies, Viral; D011993:Recombinant Fusion Proteins; D022261:Respiratory Syncytial Virus Vaccines; D014764:Viral Proteins