The murine A33 antigen is expressed at two distinct sites during development, the ICM of the blastocyst and the intestinal epithelium.
The expression pattern of the murine A33 antigen has been defined during development using wholemount immunohistochemistry. Two temporally and spatially distinct sites of expression were identified: the inner cell mass of the blastocyst and the endoderm cell layer of the intestinal tract where expression is initiated at E14.5 in the hindgut and subsequently extends throughout the length of the intestine. The onset of mA33 antigen expression in the gut occurs at the beginning of an extensive phase of cell movement involved in the conversion of the endoderm cell layer to a single cell layer of polarized epithelium. Expression of mA33 antigen is then maintained into adulthood, where it is a definitive marker of intestinal epithelium.
Abud
H E
HE
Ludwig Institute for Cancer Research, Melbourne Branch, Post Office Royal Melbourne Hospital, Victoria 3050, Parkville, Australia.
Johnstone
C N
CN
Tebbutt
N C
NC
Heath
J K
JK
D000818:Animals; D001755:Blastocyst / Q000276:immunology*; D004707:Endoderm / Q000276:immunology; D004848:Epithelium / Q000196:embryology / Q000276:immunology; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D007422:Intestines / Q000196:embryology* / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR
C504395:Gpa33 protein, mouse; D008562:Membrane Glycoproteins