title: Epithelial and stromal uterine cells cultured in vitro protect bovine sperm from hydrogen peroxide.
abstract: It is known that large amounts of leukocytes colonize the uterus, and that these leukocytes can produce considerable quantities of hydrogen peroxide (H2O2) and other reactive oxygen species that are toxic to sperm. It has been shown recently that oviductal fluid has a catalase that helps to maintain sperm motility. Therefore, the current experiment was performed to determine if a similar mechanism of protection exists against peroxides within uterine cells. Sperm motility and velocity were recorded after a 6h incubation in 1) conditioned media in the presence of endometrial cells, 2) conditioned media without endometrial cells, 3) control media (48h without cells) over endometrial cells, or 4) control media alone. All these treatments were performed in the presence or absence of added catalase. Conditioned media, endometrial cells and catalase had a significant positive effect on the maintenance of sperm motility and velocity. Addition of anti-catalase antibodies did not neutralize the beneficial effect of the conditioned media. However, the concentrations of aromatic amino acids, known substrates for sperm amino acid oxidase, were significantly lower in uterine conditioned media as compared to control medium. This reduction of aromatic amino acids was in correlation with reduced H2O2 production by sperm as estimated by chemiluminescence. These results suggest that epithelial and stromal uterine cells do not maintain sperm motility by secreting catalase in the conditioned media, but rather by reducing the levels of aromatic amino acids and thus of peroxides generated in the presence of spermatozoa.
authors.affiliation: Department of Animal Science, Laval University, Sainte-Foy, Canada.
authors.forename: S
authors.initials: S
authors.lastname: Lapointe
authors.forename: J F
authors.initials: JF
authors.lastname: Bilodeau
authors.forename: D
authors.initials: D
authors.lastname: Lemieux
authors.forename: E
authors.initials: E
authors.lastname: Asselin
authors.forename: M A
authors.initials: MA
authors.lastname: Fortier
authors.forename: M A
authors.initials: MA
authors.lastname: Sirard
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000662:veterinary*; D017077:Culture Media, Conditioned; D004847:Epithelial Cells / Q000166:cytology*; D005260:Female; D005609:Free Radicals; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008163:Luminescent Measurements; D008297:Male; D013094:Spermatozoa / Q000187:drug effects*; D014599:Uterus / Q000166:cytology*
chemical_list: D017077:Culture Media, Conditioned; D005609:Free Radicals; D006861:Hydrogen Peroxide; D002374:Catalase