title: Isolation and characterization of myogenic satellite cells from the muscular dystrophic hamster.
abstract: Myogenic satellite cells were isolated from control and dystrophic hamster diaphragms to examine cellular mechanisms involved in the physiology of muscular dystrophy. The Bio 14.6 dystrophic hamster, which possesses a defect in the delta-sarcoglycan gene, develops biochemical and physical symptoms of Duchenne-like and limb girdle muscular dystrophies. Because primary cultures of the control and dystrophic satellite cells became extensively contaminated with non-myogenic cells during proliferation, cell clones were developed to provide pure cultures for study. Cell culture conditions were optimized with the use of Ham's F-12K medium containing 10% fetal bovine serum +5% horse serum + 10 ng/mL basic fibroblast growth factor + 50 microg/mL porcine gelatin. Proliferation rates of the two clonal cultures were similar between the two lines. Satellite cell-derived myotubes from both primary cultures and clones differed between control and dystrophic animals. Dystrophic myotubes tended to be long and narrow, while the control-derived myotubes were broader. Measurement of muscle-specific creatine kinase during differentiation revealed that the dystrophic myotubes possessed higher creatine kinase levels than control myotubes (up to 146-fold at 168 h). The results demonstrate that satellite cells can be isolated from the hamster and may provide a useful tool to study muscular dystrophies associated with defects in the sarcoglycan complex and the involvement of sarcoglycans in normal skeletal muscle growth and development.
authors.affiliation: Muscle Biology Laboratories, South Dakota State University, Brookings 57007-0392, USA. Douglas_McFarland@sdstate.edu
authors.forename: D C
authors.initials: DC
authors.lastname: McFarland
authors.forename: Y N
authors.initials: YN
authors.lastname: Singh
authors.forename: A D
authors.initials: AD
authors.lastname: Johnson
authors.forename: J E
authors.initials: JE
authors.lastname: Pesall
authors.forename: K K
authors.initials: KK
authors.lastname: Gilkerson
authors.forename: L S
authors.initials: LS
authors.lastname: Vander Wal
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002455:Cell Division; D002999:Clone Cells / Q000166:cytology; D006224:Cricetinae; D008297:Male; D018482:Muscle, Skeletal / Q000166:cytology*; D009137:Muscular Dystrophy, Animal / Q000473:pathology*; D013234:Stem Cells / Q000166:cytology