Analysis of function and regulation of proteins that mediate signal transduction by use of lipid-modified plasma membrane-targeting sequences.
It is now established that the function of many signaling molecules is controlled, in part, by regulation of subcellular localization. For example, the dynamic recruitment of normally cytosolic proteins to the plasma membrane, by activated Ras or activated receptor tyrosine kinases, facilitates their interaction with other membrane-associated components that participate in their full activation (e.g., Raf-1). Therefore, the creation of chimeric proteins that contain lipid-modified signaling sequences that direct membrane localization allows the generation of constitutively activated variants of such proteins. The amino-terminal myristoylation signal sequence of Src family proteins and the carboxy-terminal prenylation signal sequence of Ras proteins have been widely used to achieve this goal. Such membrane-targeted variants have proved to be valuable reagents in the study of the biochemical and biological properties of many signaling molecules.
Reuther
G W
GW
Department of Pharmacology, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill 27599, USA.
Buss
J E
JE
Quilliam
L A
LA
Clark
G J
GJ
Der
C J
CJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001671:Biochemistry / Q000379:methods*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D006801:Humans; D050356:Lipid Metabolism; D008969:Molecular Sequence Data; D019308:Palmitic Acid / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D017368:Protein Prenylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011518:Proto-Oncogene Proteins; D051057:Proto-Oncogene Proteins c-akt; D015398:Signal Transduction
D011518:Proto-Oncogene Proteins; D019308:Palmitic Acid; D017346:Protein Serine-Threonine Kinases; D051057:Proto-Oncogene Proteins c-akt