A novel method for collecting and detecting amoxycillin in urine: a tool for testing antibiotic compliance in the community.
Assessing compliance to prescribed antibiotics in community studies of respiratory tract infections is difficult. We describe a simple method for collecting and detecting amoxycillin in urine using urine dip-sticks in conjunction with a bioassay. Urine was collected at timed intervals from eight healthy volunteers following oral amoxycillin administration. Dip-sticks inoculated with urine collected 1 and 8 h after antibiotic resulted in mean zones of inhibition of 1.75 and 1.37 cm, respectively. Amoxycillin activity remained demonstrable 14 days after inoculation of dip-sticks with urine. Dip-sticks inoculated with urine from control subjects who had not taken amoxycillin did not cause inhibition.
Lim
W S
WS
Respiratory Medicine and Department of Infectious Diseases, Nottingham City Hospital, Hucknall Road, Nottingham NG5 1PB, UK.
Gander
S
S
Finch
R G
RG
Macfarlane
J T
JT
D000658:Amoxicillin / Q000627:therapeutic use / Q000652:urine*; D001431:Bacteriological Techniques / Q000379:methods; D016903:Drug Monitoring; D005260:Female; D006801:Humans; D008297:Male; D010349:Patient Compliance; D010406:Penicillins / Q000627:therapeutic use / Q000652:urine*; D010865:Pilot Projects; D011933:Reagent Kits, Diagnostic; D012111:Residence Characteristics; D012141:Respiratory Tract Infections / Q000188:drug therapy
D010406:Penicillins; D011933:Reagent Kits, Diagnostic; D000658:Amoxicillin