Effects of echovirus 1 infection on cellular gene expression.
To obtain a view of the influence of enterovirus infection on host cell gene expression, multiple cellular mRNA levels were first investigated during echovirus 1 (EV1) infection in HOS palpha2AW cells using cDNA array analysis. Visible cytopathic effect and partial shut-off of host cell protein synthesis were observed 6-10 h after the EV1 infection. Simultaneously, approximately 2% of the investigated genes, among them immediate-early response genes and genes involved in apoptotic pathways and cell growth regulation, were activated over twofold and less than 0.5% of genes were downregulated. For comparison, the cellular effects of coxsackievirus B4 and poliovirus 1 infections were studied in HeLa-Ohio cells by cDNA arrays. Gene activation patterns detected in the host cells during the infection by the three enteroviruses were only partially similar.
Pietiäinen
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V
Haartman Institute, University of Helsinki, Helsinki, FIN-00014, Finland. vilja.pietianen@helsinki.fi
Huttunen
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P
Hyypiä
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T
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D029822:Enterovirus B, Human / Q000502:physiology*; D015967:Gene Expression Regulation, Viral; D006367:HeLa Cells; D006801:Humans; D017955:Poliovirus / Q000502:physiology; D015533:Transcriptional Activation; D014407:Tumor Cells, Cultured