title: Gamma-ray-irradiated red blood cells stored in mannitol-adenine-phosphate medium: rheological evaluation and susceptibility to oxidative stress.
abstract: OBJECTIVE
  To evaluate the rheological properties and the oxidative susceptibility of gamma-ray-irradiated red blood cells (RBCs).
  
  
  METHODS
  RBCs in mannitol-adenine-phosphate (MAP) medium were irradiated with 35 Gy and stored at 4 degrees C for 4 weeks. The deformability of the RBCs was examined under shear flow in relation to the morphological and biochemical changes. The RBCs were further exposed to 1 mM FeSO(4) and 5 mM ascorbate to examine the oxidative susceptibility.
  
  
  RESULTS
  The RBC deformability was decreased during storage, and the impairment was further enhanced by the irradiation, which promoted cell shrinkage and intracellular hemoglobin condensation accompanying potassium loss. Lipid peroxidation and protein aggregation of the RBC membrane as well as echinocytosis were not enhanced by the irradiation. The exposure to free iron did not stimulate the oxidation of the irradiated RBC membrane.
  
  
  CONCLUSIONS
  The decreased deformability of gamma-ray-irradiated RBCs in MAP medium was mainly induced by dehydration due to potassium loss, and the membrane lipids and proteins were stably preserved against oxidative stress.
authors.affiliation: Department of Physiology, Ehime University School of Medicine, Ehime, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Cicha
authors.forename: Y
authors.initials: Y
authors.lastname: Suzuki
authors.forename: N
authors.initials: N
authors.lastname: Tateishi
authors.forename: M
authors.initials: M
authors.lastname: Shiba
authors.forename: M
authors.initials: M
authors.lastname: Muraoka
authors.forename: K
authors.initials: K
authors.lastname: Tadokoro
authors.forename: N
authors.initials: N
authors.lastname: Maeda
mesh_terms: D000225:Adenine / Q000494:pharmacology*; D001793:Blood Preservation / Q000379:methods / Q000592:standards*; D004907:Erythrocyte Deformability / Q000528:radiation effects; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D005720:Gamma Rays / Q000009:adverse effects*; D006801:Humans; D017136:Ion Transport / Q000528:radiation effects; D015227:Lipid Peroxidation / Q000528:radiation effects; D008353:Mannitol / Q000494:pharmacology*; D008565:Membrane Proteins / Q000187:drug effects / Q000528:radiation effects; D018384:Oxidative Stress / Q000187:drug effects / Q000528:radiation effects; D011188:Potassium / Q000378:metabolism; D012212:Rheology / Q000528:radiation effects; D012964:Sodium / Q000378:metabolism; D013314:Stress, Mechanical; D013997:Time Factors
chemical_list: C075774:MAP solution; D008565:Membrane Proteins; D008353:Mannitol; D012964:Sodium; D000225:Adenine; D011188:Potassium