Signature-tagged mutagenesis in the identification of virulence genes in pathogens.
Signature-tagged mutagenesis is a functional genomics technique that identifies microbial genes required for infection within an animal host, or within host cells. The application of this technique to a range of microbial pathogens has resulted in the identification of novel virulence determinants in each screen performed to date, so that cumulatively several hundred genes have been ascribed a role in virulence.
Shea
J E
JE
Microscience Ltd, 545 Eskdale Road, Winnersh Triangle, RG41 5TU, Wokingham, Berkshire, UK. j.shea@microscience.com
Santangelo
J D
JD
Feldman
R G
RG
D002175:Candida / Q000235:genetics / Q000472:pathogenicity; D023281:Genomics; D006090:Gram-Negative Bacteria / Q000235:genetics / Q000472:pathogenicity; D006094:Gram-Positive Bacteria / Q000235:genetics / Q000472:pathogenicity; D016254:Mutagenesis, Insertional; D014774:Virulence / Q000235:genetics*