A CDE/CHR tandem element regulates cell cycle-dependent repression of cyclin B2 transcription.
Cyclin B is an important regulator of progression through the cell division cycle. The oscillating appearance of cyclin B1 and B2 proteins during the cell cycle is in part due to fluctuating mRNA levels. We had identified earlier a tandem promoter element named cell cycle-dependent element (CDE) and cell cycle genes homology region (CHR) which regulates cell cycle-dependent transcription of cdc25C, cyclin A and cdc2. Here we describe that cyclin B2 transcription is repressed through a novel CDE/CHR element in resting and G(1) cells. By relief of this repression in S and G(2) oscillating expression of cyclin B2 mRNA is achieved during the cell cycle.
Lange-zu Dohna
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Medizinische Klinik II, Department of Internal Medicine, University of Leipzig, Leipzig, Germany.
Brandeis
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Berr
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Mössner
J
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Engeland
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D000818:Animals; D001483:Base Sequence; D002453:Cell Cycle / Q000235:genetics; D002478:Cells, Cultured; D019926:Cyclin B / Q000235:genetics* / Q000378:metabolism; D056765:Cyclin B2; D004247:DNA / Q000032:analysis; D020868:Gene Silencing; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013539:Simian virus 40 / Q000235:genetics; D020080:Tandem Repeat Sequences / Q000235:genetics*; D014158:Transcription, Genetic
C503759:Ccnb2 protein, mouse; D019926:Cyclin B; D056765:Cyclin B2; D004247:DNA