[The mobile vector method for genetic transformation of animals].
Experimental data on transgenic animal production using transfected sperm are reviewed. Pathways for further improvement of the method for increasing the frequency of transgenic organisms are proposed: modification of the saccharophosphate skeleton and DNA ends; use of nucleic exchange enzymes and directed oligonucleotides; use of adhesive sequences (MAR elements), ori replication, and transposable elements within the transgenes; development of the embryos selection; the use of gene targeting elements; and the use of various DNA conformations. The promising use of spermatozoa as a noninvasive system of gene delivery in transgenesis and prenatal gene therapy are outlined.
Kuznetsov
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AV
State Research Center of Applied Microbiology, Obolensk, Moscow oblast, Russia.
Kuznetsova
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D000818:Animals; D030801:Animals, Genetically Modified; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004251:DNA Transposable Elements; D004622:Embryo, Mammalian / Q000378:metabolism; D004625:Embryo, Nonmammalian; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D008297:Male; D008845:Microinjections; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000235:genetics; D018741:Replication Origin; D013094:Spermatozoa / Q000378:metabolism
D004251:DNA Transposable Elements; D009841:Oligonucleotides; D004247:DNA