1,25-Dihydroxycholecalciferol prevents and ameliorates symptoms of experimental murine inflammatory bowel disease.
Anecdotal data suggest that the amount of vitamin D available in the environment either from sunshine exposure or diet may be an important factor affecting the development of inflammatory bowel disease (IBD) in humans. We tested the vitamin D hypothesis in an experimental animal model of IBD. Interleukin (IL)-10 knockout (KO) mice, which spontaneously develop symptoms resembling human IBD, were made vitamin D deficient, vitamin D sufficient or supplemented with active vitamin D (1,25-dihydroxycholecalciferol). Vitamin D-deficient IL-10 KO mice rapidly developed diarrhea and a wasting disease, which induced mortality. In contrast, vitamin D-sufficient IL-10 KO mice did not develop diarrhea, waste or die. Supplementation with 50 IU of cholecalciferol (5.0 microgram/d) or 1, 25-dihydroxycholecalciferol (0.005 microgram/d) significantly (P < 0. 05) ameliorated symptoms of IBD in IL-10 KO mice. 1, 25-Dihydroxycholecalciferol treatment (0.2 microgram/d) for as little as 2 wk blocked the progression and ameliorated (P < 0.05) symptoms in IL-10 KO mice with already established IBD.
Cantorna
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MT
Department of Nutrition, College of Health and Human Development, The Pennsylvania State University, University Park, PA 16802, USA.
Munsick
C
C
Bemiss
C
C
Mahon
B D
BD
D000704:Analysis of Variance; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002117:Calcitriol / Q000627:therapeutic use*; D002118:Calcium / Q000097:blood; D004195:Disease Models, Animal; D005260:Female; D005838:Genotype; D015212:Inflammatory Bowel Diseases / Q000188:drug therapy* / Q000209:etiology* / Q000517:prevention & control; D016753:Interleukin-10 / Q000235:genetics; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D014808:Vitamin D Deficiency / Q000150:complications* / Q000188:drug therapy* / Q000401:mortality
D016753:Interleukin-10; D002117:Calcitriol; D002118:Calcium