title: Losses of heterozygosity in endometrial adenocarcinomas: positive correlations with histopathological parameters.
abstract: We analyzed 37 samples of endometrial adenocarcinoma for loss of heterozygosity (LOH) by using a panel of 44 microsatellites located in 29 chromosomal regions. The aim of our study was to investigate the existence of a possible preferential involvement of some tumor suppressor genes in endometrial carcinogenesis. The analysis was performed on tumoral tissue and on a corresponding normal tissue by the use of polymerase chain reaction (PCR) and the comparison of the amplified alleles. We observed significative LOH (>20%) in the chromosomal regions of 2q14 (33.33%), 7q35 (24.00%), 10q22.1 (37. 50%), 11q13-q14 (44.12%), 15q26 (40.63%), 17p13 (25.71%), and 17q21. 3 (37.04%). We defined a 1-cM minimal common deletion in 11q13-q14 between D11S911 and D11S937 markers. A statistical analysis revealed a positive correlation between LOH of 11q13-q14 and clinicopathological data.
authors.affiliation: Genetica Umana, Dipartimento di Medicina, Chirurgia ed Odontoiatria, Polo San Paolo, Università degli Studi di Milano, Milan, Italy.
authors.forename: S M
authors.initials: SM
authors.lastname: Sirchia
authors.forename: E
authors.initials: E
authors.lastname: Sironi
authors.forename: F R
authors.initials: FR
authors.lastname: Grati
authors.forename: P
authors.initials: P
authors.lastname: Serafini
authors.forename: I
authors.initials: I
authors.lastname: Garagiola
authors.forename: F
authors.initials: F
authors.lastname: Rossella
authors.forename: F
authors.initials: F
authors.lastname: Dulcetti
authors.forename: G
authors.initials: G
authors.lastname: Pardi
authors.forename: S
authors.initials: S
authors.lastname: Garsia
authors.forename: G
authors.initials: G
authors.lastname: Simoni
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000328:Adult; D000368:Aged; D002880:Chromosomes, Human, Pair 11; D016889:Endometrial Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D006801:Humans; D019656:Loss of Heterozygosity; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity