Transcriptional co-regulation of five chitinase genes scattered on the Streptomyces coelicolor A3(2) chromosome.
Streptomyces coelicolor A3(2) strain M145 has eight chitinase genes scattered on the chromosome: six genes for family 18 (chiA, B, C, D, E and H) and two for family 19 (chiF and G). In this study, the expression and regulation of these genes were investigated. The transcription of five of the genes (chiA, B, C, D and F) was induced in the presence of colloidal chitin while that of the other three genes (chiE, G and H) was not. The transcripts of the five induced chi genes increased and reached their maximum at 4 h after the addition of colloidal chitin, all showing the same temporal patterns. The induced levels of the transcripts of chiB were significantly lower than those of the other four genes. Dynamic analysis of the transcripts of the chi genes indicated that chiA and chiC were induced more strongly than chiD and chiF. Addition of chitobiose also induced transcription of the chi genes, but significantly earlier than did colloidal chitin. When cells were cultured in the presence of colloidal chitin, an exponential increase of chitobiose concentration in the culture supernatant was observed prior to the induced transcription of the chi genes. This result, together with the immediate effect of chitobiose on the induction, suggests that chitobiose produced from colloidal chitin is involved in the induction of transcription of the chi genes. The transcription of the five chi genes was repressed by glucose. This repression was apparently mediated by the glucose kinase gene glkA.
Saito
Akihiro
A
National Institute of Agro-Environmental Sciences, Kan-nondai 3-1-1, Tsukuba, Ibaraki 305-8604, Japan1.
Ishizaka
Masumi
M
National Institute of Agro-Environmental Sciences, Kan-nondai 3-1-1, Tsukuba, Ibaraki 305-8604, Japan1.
Francisco
Perigio B
PB
National Institute of Agro-Environmental Sciences, Kan-nondai 3-1-1, Tsukuba, Ibaraki 305-8604, Japan1.
Fujii
Takeshi
T
National Institute of Agro-Environmental Sciences, Kan-nondai 3-1-1, Tsukuba, Ibaraki 305-8604, Japan1.
Miyashita
Kiyotaka
K
National Institute of Agro-Environmental Sciences, Kan-nondai 3-1-1, Tsukuba, Ibaraki 305-8604, Japan1.
D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D002686:Chitin / Q000378:metabolism / Q000494:pharmacology; D002688:Chitinases / Q000235:genetics* / Q000378:metabolism; D002876:Chromosomes, Bacterial / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004187:Disaccharides / Q000096:biosynthesis / Q000494:pharmacology; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005798:Genes, Bacterial; D005941:Glucokinase / Q000235:genetics; D005947:Glucose / Q000494:pharmacology; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012689:Sequence Homology, Nucleic Acid; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects
D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D004187:Disaccharides; D002686:Chitin; C032438:chitobiose; C096446:GlkA protein, Bacteria; D005941:Glucokinase; D002688:Chitinases; D005947:Glucose