title: Comparative molecular analysis of erythromycin-resistance determinants in staphylococcal isolates of poultry and human origin.
abstract: The ermA, ermB, ermC and msrA/msrB genes were detected in multidrug-resistant Staphylococcus spp. strains by PCR. Among 25 human clinical staphylococcal isolates the ermA, ermB, ermC and the msrA/msrB genes were detected in 88, 72, 4 and 100% of the strains, respectively. Among 24 poultry isolates the ermA, ermB, ermC and the msrA/msrB genes were detected in 100, 16.6, 50 and 12.5% of the strains, respectively. The ermA gene was found exclusively on the chromosome, whereas the ermC gene was found on 2.4-4.2 kb plasmids. Restriction fragment length polymorphism (RFLP) analysis of the ermA gene with Eco RI revealed five patterns (25.0, 21.0, 10.5, 6.2 and 4. 8 kb) for the clinical strains and two (8.0 and 6.2 kb) for the poultry strains. The 6.2 kb RFLP pattern, in both the poultry and human clinical isolates, indicates a common lineage for the ermA gene.
authors.affiliation: Division of Microbiology, National Center for Toxicological Research/FDA, 3900 NCTR Road, Jefferson, AR 72079, USA. mnawaz@nctr.fda.gov
authors.forename: M S
authors.initials: MS
authors.lastname: Nawaz
authors.forename: S A
authors.initials: SA
authors.lastname: Khan
authors.forename: A A
authors.initials: AA
authors.lastname: Khan
authors.forename: F M
authors.initials: FM
authors.lastname: Khambaty
authors.forename: C E
authors.initials: CE
authors.lastname: Cerniglia
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D015246:Deoxyribonuclease EcoRI / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics*; D018432:Drug Resistance, Multiple / Q000235:genetics; D004917:Erythromycin / Q000494:pharmacology*; D018628:Gene Dosage; D005819:Genetic Markers; D006801:Humans; D026901:Membrane Transport Proteins; D008780:Methyltransferases / Q000235:genetics; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D011200:Poultry / Q000382:microbiology*; D013210:Staphylococcus / Q000187:drug effects* / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D005819:Genetic Markers; D026901:Membrane Transport Proteins; C066202:msrA protein, Staphylococcus epidermidis; D004917:Erythromycin; D008780:Methyltransferases; C035181:rRNA (adenosine-O-2'-)methyltransferase; D015246:Deoxyribonuclease EcoRI