title: Atherosclerosis-induced chronic arterial insufficiency causes clitoral cavernosal fibrosis in the rabbit.
abstract: In our previous studies we found that aging-associated fibrosis of clitoral cavernosal tissue correlated with the prevalence of cardiovascular disease in elderly women. The aim of this study was to determine specifically, arterial insufficiency-related structural changes of clitoral cavernosal tissue in a rabbit model. New Zealand white female rabbits were divided into clitoral cavernosal ischemia (CCI, n = 5) and control (n = 5) groups. The CCI group underwent balloon endothelial injury of the iliac arteries and received 0.5% cholesterol diet. The control group received a regular diet. After 16 weeks, arteriography was performed then the animals were sacrificed. The iliac arteries and the entire clitoris were removed. Cross-sections of the iliac arteries and clitoris were processed for histologic evaluation The percentage of smooth muscle and connective tissue in trichrome stained sections of clitoral cavernosal tissue was determined by computer-assisted histomorphometry. Arteriography revealed diffused occlusive disease in the common iliac, internal iliac and pudendal arteries in the CCI group. Histology showed that arterial occlusive disease spreads from the site of balloon injury to the smaller branches involving the clitoral cavernosal arteries. Diffuse fibrosis was observed in the clitoral cross-sections of the CCI group. The percentage of clitoral cavernosal smooth muscle (mean +/- standard error) in the CCI group (53% +/- 0.9%) was significantly decreased compared with the control group (62% +/- 0.8%) (P = 0.0001). Chronic clitoral cavernosal ischemia causes significant fibrosis and loss of smooth muscle in the clitoral cavernosal tissue. These findings suggest that chronic clitoral cavernosal arterial insufficiency may play a role in the pathophysiology of female sexual arousal disorders.
authors.affiliation: Department of Urology, Boston University School of Medicine, Massachusetts, USA.
authors.forename: K
authors.initials: K
authors.lastname: Park
authors.forename: T
authors.initials: T
authors.lastname: Tarcan
authors.forename: I
authors.initials: I
authors.lastname: Goldstein
authors.forename: M B
authors.initials: MB
authors.lastname: Siroky
authors.forename: R J
authors.initials: RJ
authors.lastname: Krane
authors.forename: K M
authors.initials: KM
authors.lastname: Azadzoi
mesh_terms: D000792:Angiography; D000818:Animals; D001158:Arteries / Q000473:pathology; D001161:Arteriosclerosis / Q000150:complications*; D002404:Catheterization; D002987:Clitoris / Q000098:blood supply* / Q000473:pathology; D004730:Endothelium, Vascular / Q000473:pathology; D005260:Female; D005355:Fibrosis; D007083:Iliac Artery; D007511:Ischemia / Q000209:etiology*; D009130:Muscle, Smooth / Q000098:blood supply* / Q000473:pathology*; D011817:Rabbits