title: Treatment of corneal neovascularization with dietary isoflavonoids and flavonoids.
abstract: The purpose of this study was to investigate the use of dietary isoflavonoids and flavonoids for the treatment of ocular neovascularization. Corneal blood vessels were induced by intrastromal implantation of pellets containing bFGF. Isoflavonoids and flavonoids (Genistein, Fisetin and Luteolin) were dissolved in a microemulsion to increase bioavailability and applied topically in concentrations between 0.5 and 1 ng ml(-1). Corneal neovascularization was quantified under the microscope. In comparison to control eyes, all three substances significantly inhibited corneal neovascularization (P < or = 0.05). Fisetin had the strongest effect followed by Genistein and Luteolin. No significant topical side effects were observed. We concluded that the isoflavonoid Genistein and two structurally related flavonoids are potent inhibitors of corneal angiogenesis in vivo. The wide distribution of the flavonoids in the plant kingdom together with the presented results suggests that flavonoids may contribute to the preventive effect of a plant-based diet on neovascular disease of the eye.
authors.affiliation: Department of Ophthalmology, University of Aachen (RWTH), Aachen, Germany. JoussenA@aol.com
authors.forename: A M
authors.initials: AM
authors.lastname: Joussen
authors.forename: K
authors.initials: K
authors.lastname: Rohrschneider
authors.forename: J
authors.initials: J
authors.lastname: Reichling
authors.forename: B
authors.initials: B
authors.lastname: Kirchhof
authors.forename: F E
authors.initials: FE
authors.lastname: Kruse
mesh_terms: D000287:Administration, Topical; D000818:Animals; D001682:Biological Availability; D016510:Corneal Neovascularization / Q000628:therapy*; D004655:Emulsions; D005419:Flavonoids / Q000493:pharmacokinetics / Q000627:therapeutic use*; D044948:Flavonols; D019833:Genistein / Q000493:pharmacokinetics / Q000627:therapeutic use*; D006131:Growth Inhibitors / Q000493:pharmacokinetics / Q000627:therapeutic use*; D047311:Luteolin; D011817:Rabbits; D016896:Treatment Outcome
chemical_list: D004655:Emulsions; D005419:Flavonoids; D044948:Flavonols; D006131:Growth Inhibitors; D019833:Genistein; D047311:Luteolin; C017875:fisetin