title: Enoate reductases of Clostridia. Cloning, sequencing, and expression.
abstract: The enr genes specifying enoate reductases of Clostridium tyrobutyricum and Clostridium thermoaceticum were cloned and sequenced. Sequence comparison shows that enoate reductases are similar to a family of flavoproteins comprising 2,4-dienoyl-coenzyme A reductase from Escherichia coli and old yellow enzyme from yeast. The C. thermoaceticum enr gene product was expressed in recombinant Escherichia coli cells growing under anaerobic conditions. The recombinant enzyme was purified and characterized.
authors.affiliation: Institut für Organische Chemie und Biochemie, Technische Universität München, Lichtenbergstrasse 4, D-85747 Garching, Germany. felix.rohdich@ch.tum.de
authors.forename: F
authors.initials: F
authors.lastname: Rohdich
authors.forename: A
authors.initials: A
authors.lastname: Wiese
authors.forename: R
authors.initials: R
authors.lastname: Feicht
authors.forename: H
authors.initials: H
authors.lastname: Simon
authors.forename: A
authors.initials: A
authors.lastname: Bacher
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003013:Clostridium / Q000201:enzymology*; D044943:Fatty Acid Desaturases / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D044925:Oxidoreductases Acting on CH-CH Group Donors; D011994:Recombinant Proteins / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid
chemical_list: D011994:Recombinant Proteins; D044943:Fatty Acid Desaturases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C100153:2,4-dienoyl-CoA reductase