title: No evidence of somatic activating mutations on gonadotropin receptor genes in sex cord stromal tumors.
abstract: OBJECTIVE
  To search for somatic activating mutations of gonadotropin receptor (FSH-R and LH/chorionic gonadotropin receptor [CG-R]) genes as a cause of sex cord stromal tumors.
  
  
  METHODS
  Molecular studies in human tissue.
  
  
  METHODS
  University hospital. SPECIMEN(S): Eight granulosa cell tumors collected from paraffin-embedded tissue, eight Leydig cell tumors, and three thecomas collected from fresh-frozen or paraffin-embedded tissue.
  
  
  METHODS
  Tumor samples were used for DNA extraction. The entire exon 11 of the LH/CG-R gene and a hot spot for gonadotropin receptor activating mutations on exon 10 of the FSH-R gene were amplified by polymerase chain reaction. The former was analyzed by denaturing gradient gel electrophoresis and automatic direct sequencing, and the latter by automatic direct sequencing.
  
  
  METHODS
  Results of denaturing gradient gel electrophoresis and automatic direct sequencing.
  
  
  RESULTS
  No somatic activating mutation was detected in exon 11 of the LH/CG-R gene in eight Leydig cell tumors and three thecomas. In addition, no mutations were detected in eight granulosa cell tumors in the hot spot for activating mutations in exon 10 of the FSH-R gene.
  
  
  CONCLUSIONS
  Somatic activating mutations of gonadotropin receptors seem to play no relevant role in the development of sex cord stromal tumors.
authors.affiliation: Division of Endocrinology, Developmental Endocrinology Unit and Hormone and Molecular Genetics Laboratory, Hospital das Clinicas, University of São Paulo Medical School, Brazil. anacl.usp.br
authors.forename: L R
authors.initials: LR
authors.lastname: Giacaglia
authors.forename: F
authors.initials: F
authors.lastname: Kohek MB da
authors.forename: F M
authors.initials: FM
authors.lastname: Carvalho
authors.forename: M C
authors.initials: MC
authors.lastname: Fragoso
authors.forename: B
authors.initials: B
authors.lastname: Mendonca
authors.forename: A C
authors.initials: AC
authors.lastname: Latronico
mesh_terms: D005091:Exons / Q000235:genetics; D005260:Female; D006106:Granulosa Cell Tumor / Q000235:genetics; D006801:Humans; D007984:Leydig Cell Tumor / Q000235:genetics; D008297:Male; D009154:Mutation / Q000502:physiology*; D010051:Ovarian Neoplasms / Q000235:genetics*; D011962:Receptors, FSH / Q000235:genetics*; D011974:Receptors, LH / Q000235:genetics*; D018312:Sex Cord-Gonadal Stromal Tumors / Q000235:genetics*; D013736:Testicular Neoplasms / Q000235:genetics*; D013798:Thecoma / Q000235:genetics
chemical_list: D011962:Receptors, FSH; D011974:Receptors, LH