[Application of intermittent-feeding of growth-limiting nutrients in suspension culture of insect cells(Sf21)].
On the basis of the growth and metabolism behavior inherent in suspended Sf21 insect cells, the intermittent feeding of growth-limiting nutrients(glucose and protein hydrolysates) was employed in the regulation of cellular growth during the cultivation by using the residual glucose concentration as a reference point. It was shown that as compared with the batch cultivation, the intermittent-feeding of growth-limiting nutrients effectively prolonged the growth and stationary phase for Sf21 insect cells grown in two representative culture medium(TC-100 and IPL-41). The maximum cell density was increased from 3.0 x 10(6) cells/ml to 6.5 x 10(6) cells/mL in TC-100 medium, and in IPL-41 medium, the maximum cell density was increased from 7.05 x 10(6) cells/mL to 9.0 x 10(6) cells/mL. As the defined nutrient solution was used for feeding in lieu of the complicated and expensive base medium, the technique would find prospects in large scale high-density cultivation of insect cells.
Zhao
J
J
State Key Laboratory of Bioreactor Engineering, ECUST, Shanghai.
Tan
W S
WS
Zhou
Y
Y
Zhou
L
L
Yu
J T
JT
D000818:Animals; D002452:Cell Count; D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media; D018411:Spodoptera / Q000166:cytology*; D013535:Suspensions
D003470:Culture Media; D013535:Suspensions