Crystallization and preliminary X-ray studies of oligandrin, a sterol-carrier elicitor from Pythium oligandrum.
Oligandrin is a 10 kDa acidic protein produced by the fungus micromycete Pythium oligandrum and is a member of the alpha-elicitin group, with sterol- and lipid-carrier properties. Oligandrin has been crystallized at 290 K using PEG 4000 as a precipitant. A cholesterol complex was obtained under the same conditions. The space group of the crystals at low temperature (100 K) is C222, with unit-cell parameters a = 94.0, b = 171.1, c = 55.3 A. Four molecules are present in the asymmetric unit. Data from the free and cholesterol-complexed forms were recorded at synchrotron sources to resolutions of 2.4 (uncomplexed) and 1.9 A (complexed), respectively.
Lascombe
M B
MB
LCRB - Laboratoire de Cristallographie et RMN Biologiques (EP-2075, CNRS), Faculté de Pharmacie, 4 Avenue de l'Observatoire, 75270 Paris CEDEX 06, France.
Milat
M L
ML
Blein
J P
JP
Panabières
F
F
Ponchet
M
M
Prangé
T
T
D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism; D002784:Cholesterol / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification; D036341:Intercellular Signaling Peptides and Proteins; D011487:Protein Conformation; D011775:Pythium / Q000737:chemistry*; D013261:Sterols / Q000378:metabolism*
D002352:Carrier Proteins; D005656:Fungal Proteins; D036341:Intercellular Signaling Peptides and Proteins; C415980:Oligandrin protein, Pythium oligandrum; D013261:Sterols; D002784:Cholesterol