title: Catabolite repression of dra-nupC-pdp operon expression in Bacillus subtilis.
abstract: Expression of the Bacillus subtilis dra-nupC-pdp operon is subject to catabolite repression by glucose. It was shown that a cis-acting catabolite-responsive element (CRE) sequence located 64 bp downstream of the transcription-start site mediated catabolite repression of the dra-nupC-pdp operon as it does for many other B. subtilis genes. Point mutations in the CRE sequence caused the loss of catabolite repression of the operon. Catabolite repression of dra-nupC-pdp expression was relieved in a ccpA mutant and was found to be dependent on both HPr and the HPr-like protein Crh. Furthermore, a transcription-repair coupling factor, Mfd, was also found to be involved in the glucose repression of dra-nupC-pdp expression. By the use of in vitro gel mobility shift analysis, a specific HPr-P dependent binding of CcpA to the dra CRE site was demonstrated.
authors.affiliation: Department of Microbiology, Technical University of Denmark, Building 301, DK-2800 Lyngby, Denmark1.
authors.forename: Xianmin
authors.initials: X
authors.lastname: Zeng
authors.affiliation: Institut de Biologie et Chimie des Protéines, CNRS, 7 Passage du Vercors, F-69376 Lyon, Cedex 07, France2.
authors.forename: Anne
authors.initials: A
authors.lastname: Galinier
authors.affiliation: Department of Microbiology, Technical University of Denmark, Building 301, DK-2800 Lyngby, Denmark1.
authors.forename: Hans H
authors.initials: HH
authors.lastname: Saxild
mesh_terms: D001412:Bacillus subtilis / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005798:Genes, Bacterial; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D026901:Membrane Transport Proteins; D016297:Mutagenesis, Site-Directed; D009876:Operon; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D017354:Point Mutation; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D017384:Sequence Deletion; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; C118042:Crh protein, Bacillus subtilis; D017931:DNA Primers; D004268:DNA-Binding Proteins; D026901:Membrane Transport Proteins; C088081:NupC protein, Bacteria; D010750:Phosphoproteins; D012097:Repressor Proteins; D014157:Transcription Factors; C069286:catabolite control proteins, bacteria; C072103:transcription repair coupling factor protein, Bacteria; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C030182:phosphocarrier protein HPr; D005947:Glucose