In vitro analysis of the change in resistance of Chlamydia trachomatis under exposure to sub-MIC levofloxacin for a therapeutic term.
BACKGROUND
While fluoroquinolone-resistant Chlamydia trachomatis strains have not been clinically isolated, they were isolated in an in vitro study recently.


METHODS
To determine whether C. trachomatis strains develop resistance under sub-MIC antibacterial exposure in a clinical therapeutic term, C. trachomatis strains were exposed to sub-MIC levofloxacin (LVFX) for about 2 weeks. The MIC of LVFX was measured and DNA fingerprinting was performed every 72 h by PCR using random primers.


RESULTS
There was almost no change in the MIC under exposure to 0.125 microg/ml LVFX. However, some mutational changes in DNA fingerprints developed.


CONCLUSIONS
In clinical therapeutic terms, resistant strains of C. trachomatis will probably not develop, even if sub-MIC LVFX is employed.
Takahashi
S
S
Department of Urology, Sapporo Medical University School of Medicine, Sapporo, Japan.
Hagiwara
T
T
Shiga
S
S
Takaoki Hirose
T
T
Tsukamoto
T
T
D000890:Anti-Infective Agents / Q000494:pharmacology*; D002692:Chlamydia trachomatis / Q000187:drug effects* / Q000235:genetics; D017931:DNA Primers; D004352:Drug Resistance, Microbial / Q000235:genetics; D064704:Levofloxacin; D008826:Microbial Sensitivity Tests / Q000379:methods; D015242:Ofloxacin / Q000494:pharmacology*; D019105:Random Amplified Polymorphic DNA Technique; D012641:Selection, Genetic
D000890:Anti-Infective Agents; D017931:DNA Primers; D064704:Levofloxacin; D015242:Ofloxacin