Growth on indium-tin oxide-coated glass enhances 32P-phosphate uptake and protein labelling of adherent cells.
A method to improve the efficiency of labelling of adherent cells with radioactive 32p is described. Cells are grown on a glass surface which is coated with indium-tin oxide, a commercially available, transparent material which permits excellent cell adhesion and growth. The results show that a 2 to 3-fold increase in 32p uptake by the cells can be achieved by growing cells on this material, compared to conventional tissue culture plastic.
Tomai
E
E
Queen's University, Dept of Microbiology and Immunology, Kingston, Ont, Canada.
Klein
S
S
Firth
K
K
Raptis
L
L
D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000378:metabolism; D007204:Indium / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry; D010761:Phosphorus Radioisotopes / Q000737:chemistry / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats; D017971:Tin Compounds / Q000737:chemistry*
D010710:Phosphates; D010761:Phosphorus Radioisotopes; D017971:Tin Compounds; D007204:Indium; C045358:stannic oxide