Quantitative in vitro bioassay for recombinant human interleukin-11.
A cell culture-based in vitro bioassay was developed to measure the biological activity of recombinant human interleukin-11 (rhIL-11). The bioassay measures induced proliferation of T10 cells, derived from the T1165 murine plasmacytoma line. A colorimetrically detectable formazan product, obtained by cellular reduction of the tetrazolium compound, 4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate (WST-1), was used as an endpoint for response of clone T10 to added rhIL-11. Positions of the samples and the standards in 96-well microplates affected the precision of this bioassay, which was improved by using 2 microplates where serially diluted sample and standard lines were interleaved and their positions were alternated. The coefficient of variation for this bioassay was less than 8%. This method is suitable for quality control of rhIL-11 because of its simplicity, reproducibility, and accuracy.
Yokota
H
H
Yamanouchi Pharmaceutical Co., Ltd, Tsukuba-shi, Japan.
Kishimoto
M
M
Saito
H
H
Sakai
T
T
Yokota
S
S
Kojima
S
S
Taniguchi
Y
Y
Kaniwa
H
H
Saisho
N
N
D000818:Animals; D001681:Biological Assay; D002138:Calibration; D002460:Cell Line; D003124:Colorimetry; D006801:Humans; D006825:Hybridomas; D007202:Indicators and Reagents; D017370:Interleukin-11 / Q000032:analysis*; D051379:Mice; D011786:Quality Control; D011994:Recombinant Proteins / Q000032:analysis; D015203:Reproducibility of Results
D007202:Indicators and Reagents; D017370:Interleukin-11; D011994:Recombinant Proteins