HgCl2 disrupts the structure of the human erythrocyte membrane and model phospholipid bilayers.
The structural effects of Hg(II) ions on the erythrocyte membrane were studied through the interactions of HgCl2 with human erythrocytes and their isolated resealed membranes. Studies were carried out by scanning electron microscopy and fluorescence spectroscopy, respectively. Hg(II) induced shape changes in erythrocytes, which took the form of echinocytes and stomatocytes. This finding means that Hg(II) locates in both the outer and inner monolayers of the erythrocyte membrane. Fluorescence spectroscopy results indicate strong interactions of Hg(II) ions with phospholipid amino groups, which also affected the packing of the lipid acyl chains at the deep hydrophobic core of the membrane. HgCl2 also interacted with bilayers of dimyristoylphosphatidylcholine and dimyristoylphosphatidylethanolamine, representative of phospholipid classes located in the outer and inner monolayers of the erythrocyte membrane, respectively. X-ray diffraction indicated that Hg(II) ions induced molecular disorder to both phospholipid bilayers, while fluorescence spectroscopy of dimyristoylphosphatidylcholine large unilamellar vesicles confirmed the interaction of Hg(II) ions with the lipid polar head groups. All these findings point to the important role of the phospholipid bilayers in the interaction of Hg(II) on cell membranes.
Suwalsky
M
M
Faculty of Chemical Sciences, University of Concepción, Chile. msuwalsk@udec.cl
Ungerer
B
B
Villena
F
F
Cuevas
F
F
Sotomayor
C P
CP
D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000648:ultrastructure*; D006801:Humans; D066298:In Vitro Techniques; D008051:Lipid Bilayers / Q000737:chemistry*; D008297:Male; D008627:Mercuric Chloride / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D014961:X-Ray Diffraction
D008051:Lipid Bilayers; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D008627:Mercuric Chloride; D004134:Dimyristoylphosphatidylcholine; C018525:1,2-dimyristoylphosphatidylethanolamine