Trypanosoma brucei: four tandemly linked genes for fatty acyl-CoA synthetases.
We have cloned four acyl CoA synthetase (ACS) genes from Trypanosoma brucei strain 927. Each of these genes encodes a polypeptide about 78 kDa in size and all four contain the "ACS signature motif." Sequence alignments indicate that these proteins are 46%-95% identical in amino acid sequence. Interestingly, three of them share almost identical C-termini (about 215 amino acid residues). Southern blots suggest that these genes are present in a single copy, and Northern blots reveal that all four are expressed in both bloodstream and procyclic trypanosomes.
Jiang
D W
DW
Department of Biological Chemistry, Johns Hopkins Medical School, Baltimore, MD 21205, USA.
Ingersoll
R
R
Myler
P J
PJ
Englund
P T
PT
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003066:Coenzyme A Ligases / Q000737:chemistry / Q000235:genetics*; D016054:DNA, Protozoan / Q000737:chemistry; D015971:Gene Expression Regulation, Enzymologic; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014346:Trypanosoma brucei brucei / Q000201:enzymology* / Q000235:genetics*
D016054:DNA, Protozoan; D003066:Coenzyme A Ligases