[Typing of STR locus in biological objects with pronounced degeneration: analysis of DNA in exhumed remains of victims of war actions].
The authors describe some specific features of enzymatic amplification typing of DNA preparations obtained from degraded tissues of remains of humans, which were brought from regions of war actions in the Chechen Republic. Special attention is paid to the specific artefact of polymerase chain reaction, for the first time detected by the authors in examinations of the above-mentioned objects. This so-called "ladder" effect manifesting by simultaneous nonspecific amplification of many variants of allele fragments of the STR locus on the individual DNA matrix, which can be erroneously interpreted as an evidence of mixed DNA preparation, is apparently characteristic of individual objects with pronounced degradation of biological material. Such phenomena were observed in typing of STR locuses in biological tissues subjected to biological, thermal, and physicohemical degradation. The phenomenon was studied in detail.
Isaenko
M V
MV
Ivanov
P L
PL
D001842:Bone and Bones / Q000737:chemistry*; D003238:Connective Tissue / Q000737:chemistry*; D004247:DNA / Q000032:analysis*; D005554:Forensic Medicine / Q000379:methods*; D006801:Humans; D008967:Molecular Biology / Q000379:methods*; D016133:Polymerase Chain Reaction; D012426:Russia; D014857:Warfare
D004247:DNA