Analysis of glutathionyl hemoglobin levels in diabetic patients by electrospray ionization liquid chromatography-mass spectrometry: effect of vitamin E administration.
By using electrospray ionization liquid chromatography-mass spectrometry, we demonstrated that glutathionyl hemoglobin (Hb)beta-chain levels are markedly increased in the erythrocytes of diabetic patients as compared with healthy subjects. The administration of vitamin E to the diabetic patients for 8 weeks significantly decreased the levels of glutathionyl Hbbeta, whereas it did not affect the levels of HbA1c, glycated Hbbeta or glycated Hbalpha. Glutathionyl Hb levels can be used as a new clinical marker of oxidative stress.
Naito
C
C
Nagoya University Daiko Medical Center, Japan.
Niwa
T
T
D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D005978:Glutathione / Q000032:analysis*; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014810:Vitamin E / Q000008:administration & dosage*
D006454:Hemoglobins; C404464:glutathionyl hemoglobin; D014810:Vitamin E; D005978:Glutathione