title: Glucocorticoid and Fas ligand induced mucosal lymphocyte apoptosis after burn injury.
abstract: BACKGROUND
  The purpose of this study was to examine the effects of a steam burn injury on apoptosis in gut-associated lymphoid tissue and to determine whether endogenous glucocorticoid and Fas ligand signaling were involved in this process.
  
  
  METHODS
  Histologic analysis, in situ deoxynucleotidyl transferase dUTP nick-end labeling staining and annexin V and 7-amino-actinomycin-D flow cytometry of lymphocyte populations were evaluated in intraepithelial lymphocytes and Peyer's patch. Additional mice were pretreated with a glucocorticoid receptor antagonist (mifepristone) before the steam burn. Similarly, C3H/HeJ-FasL(gld) mice lacking functional Fas ligand were also studied.
  
  
  RESULTS
  Apoptosis was significantly increased in intraepithelial lymphocytes and Peyer's patch after the burn injury. Mifepristone pretreatment significantly reduced apoptosis in both T- and B-cell populations in intraepithelial lymphocytes after the burn injury. In contrast, the increased apoptosis seen in B-cells from Peyer's patch was not seen in C3H/HeJFasL(gld) mice, whereas the increased apoptosis in CD8+ T-cells was unaffected.
  
  
  CONCLUSIONS
  Both corticosteroids and FasL contribute to the apoptosis in gut-associated lymphoid tissues early after burn injury.
authors.affiliation: Department of Surgery, University of Florida College of Medicine, Gainesville 32610-0286, USA.
authors.forename: K
authors.initials: K
authors.lastname: Fukuzuka
authors.forename: C K
authors.initials: CK
authors.lastname: Edwards
authors.forename: M
authors.initials: M
authors.lastname: Clare-Salzer
authors.forename: E M
authors.initials: EM
authors.lastname: Copeland
authors.forename: L L
authors.initials: LL
authors.lastname: Moldawer
authors.forename: D W
authors.initials: DW
authors.lastname: Mozingo
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000276:immunology*; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D002056:Burns / Q000276:immunology* / Q000473:pathology; D004195:Disease Models, Animal; D053222:Fas Ligand Protein; D005260:Female; D005938:Glucocorticoids / Q000378:metabolism* / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D008221:Lymphoid Tissue / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D015735:Mifepristone / Q000494:pharmacology; D010581:Peyer's Patches / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism
chemical_list: D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; D005938:Glucocorticoids; D008562:Membrane Glycoproteins; D015735:Mifepristone