title: Core histone acetylation is regulated by linker histone stoichiometry in vivo.
abstract: We investigated the relationship between linker histone stoichiometry and the acetylation of core histones in vivo. Exponentially growing cell lines induced to overproduce either of two H1 variants, H1(0) or H1c, displayed significantly reduced rates of incorporation of [(3)H]acetate into all four core histones. Pulse-chase experiments indicated that the rates of histone deacetylation were similar in all cell lines. These effects were also observed in nuclei isolated from these cells upon labeling with [(3)H]acetyl-CoA. Nuclear extracts prepared from control and H1-overexpressing cell lines displayed similar levels of histone acetylation activity on chromatin templates prepared from control cells. In contrast, extracts prepared from control cells were significantly less active on chromatin templates prepared from H1-overexpressing cells than on templates prepared from control cells. Reduced levels of acetylation in H1-overproducing cell lines do not appear to depend on higher order chromatin structure, because it persists even after digestion of the chromatin with micrococcal nuclease. The results suggest that alterations in chromatin structure, resulting from changes in linker histone stoichiometry may modulate the levels or rates of core histone acetylation in vivo.
authors.affiliation: Department of Biochemistry, University of Mississippi Medical Center, Jackson, Mississippi 39216, USA.
authors.forename: A
authors.initials: A
authors.lastname: Gunjan
authors.forename: D B
authors.initials: DB
authors.lastname: Sittman
authors.forename: D T
authors.initials: DT
authors.lastname: Brown
mesh_terms: D000085:Acetates / Q000378:metabolism*; D000107:Acetylation; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D002843:Chromatin / Q000737:chemistry; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D009707:Nucleosomes / Q000378:metabolism
chemical_list: D000085:Acetates; D002843:Chromatin; D006657:Histones; D009707:Nucleosomes