title: In vitro genotoxicity testing of ARASCO and DHASCO oils.
abstract: ARASCO and DHASCO oils are microbially-derived triglycerides rich in arachidonic (20:4n-6) and docosahexaenoic (22:6n-3) acids, respectively. Both oils were tested for mutagenic activity in three different in vitro mutagenesis assays. All assays were conducted with and without metabolic activation. Neither ARASCO nor DHASCO oil was mutagenic in the Ames reverse mutation assay using five different Salmonella histidine auxotroph tester strains, nor were the oils mutagenic in the mouse lymphoma TK(+/-) forward mutation assay. The oils showed no clastogenic activity in chromosomal aberration assays performed with Chinese hamster ovary cells. Based on these assays, neither ARASCO nor DHASCO oils appear to have any genotoxic potential.
authors.affiliation: Martek Biosciences Corporation, 6480 Dobbin Road, Columbia, MD 21043, USA.
authors.forename: L M
authors.initials: LM
authors.lastname: Arterburn
authors.forename: K D
authors.initials: KD
authors.lastname: Boswell
authors.forename: T
authors.initials: T
authors.lastname: Lawlor
authors.forename: M A
authors.initials: MA
authors.lastname: Cifone
authors.forename: H
authors.initials: H
authors.lastname: Murli
authors.forename: D J
authors.initials: DJ
authors.lastname: Kyle
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000032:analysis / Q000633:toxicity*; D016466:CHO Cells; D002470:Cell Survival / Q000187:drug effects; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects; D006224:Cricetinae; D004247:DNA / Q000187:drug effects; D004281:Docosahexaenoic Acids / Q000032:analysis / Q000633:toxicity*; D007940:Leukemia L5178 / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D051379:Mice; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D009821:Oils / Q000737:chemistry / Q000633:toxicity; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D014407:Tumor Cells, Cultured
chemical_list: D009153:Mutagens; D009821:Oils; D004281:Docosahexaenoic Acids; D016718:Arachidonic Acid; D004247:DNA