Primary habitual abortions are associated with high frequency of factor V Leiden mutation.
OBJECTIVE
To analyze the prevalence of the mutation G1691A in factor V gene (Leiden mutation), of mutation C677T in the methylenetetrahydrofolate reductase (MTHFR) gene, and of polymorphism in G20210A in the prothrombin gene in women with recurrent abortions; further, to identify a subgroup at higher risk of being carriers of these mutations.


METHODS
Prospective case control evaluation.


METHODS
University clinic.


METHODS
Eighty-four women with 3 or more consecutive miscarriages were compared with 69 controls.


METHODS
None.


METHODS
Polymerase chain reactions were performed to identify the mutations G1691A in factor V and C677T in MTHFR genes and the polymorphism G20210A in the prothrombin gene.


RESULTS
In women with primary habitual abortions, 27.8% carried the Leiden mutation. No difference was observed in the prevalence of mutation C677T in the MTHFR gene or in polymorphism G20210A in the prothrombin gene.


CONCLUSIONS
The Leiden mutation may play a considerable role for women having primary recurrent abortions.
Wramsby
M L
ML
Department of Woman and Child Health, Karolinska Institute, Stockholm, Sweden. margaretha.wramsby@kbh.ki.se
Sten-Linder
M
M
Bremme
K
K
D000026:Abortion, Habitual / Q000235:genetics*; D000328:Adult; D016022:Case-Control Studies; D005165:Factor V / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D006801:Humans; D042965:Methylenetetrahydrofolate Reductase (NADPH2); D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000235:genetics; D011110:Polymorphism, Genetic; D011446:Prospective Studies; D011516:Prothrombin / Q000235:genetics
C095381:factor V Leiden; D005165:Factor V; D011516:Prothrombin; D000587:Oxidoreductases Acting on CH-NH Group Donors; D042965:Methylenetetrahydrofolate Reductase (NADPH2)