title: Fluorovisualization effect of acridine orange on mouse osteosarcoma.
abstract: If the localization of musculoskeletal sarcomas could be visualized during surgery, it would be possible to completely resect the tumor with minimum damage to normal tissues and the patients could retain a functional limb. Therefore, we conducted the present study to clarify the usefulness of acridine orange (AO) for fluorovisualization of tumors using a mouse osteosarcoma model. At 2 hours after injection of 10 mg/kg AO to mice inoculated with MOS mouse osteosarcoma cells, fluorovisualization of mouse osteosarcoma reached the maximum level. Even a 1-mm-diameter lesion of pulmonary metastasis was visualized. The results suggested that AO may be useful for specific fluorovisualization of human osteosarcomas during surgery.
authors.affiliation: Department of Orthopaedic Surgery, Kyoto Prefectural University of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kusuzaki
authors.forename: T
authors.initials: T
authors.lastname: Suginoshita
authors.forename: G
authors.initials: G
authors.lastname: Minami
authors.forename: K
authors.initials: K
authors.lastname: Aomori
authors.forename: H
authors.initials: H
authors.lastname: Takeshita
authors.forename: H
authors.initials: H
authors.lastname: Murata
authors.forename: S
authors.initials: S
authors.lastname: Hashiguchi
authors.forename: T
authors.initials: T
authors.lastname: Ashihara
authors.forename: Y
authors.initials: Y
authors.lastname: Hirasawa
mesh_terms: D000165:Acridine Orange; D000818:Animals; D001859:Bone Neoplasms / Q000473:pathology*; D004195:Disease Models, Animal; D005453:Fluorescence; D005456:Fluorescent Dyes; D006801:Humans; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D012516:Osteosarcoma / Q000473:pathology*
chemical_list: D005456:Fluorescent Dyes; D000165:Acridine Orange