UVAI-induced edema and pyrimidine dimers in murine skin.
The induction of edema and pyrimidine dimers in epidermal DNA was determined in the skin of SKH:HR1 mice exposed to graded doses of ultraviolet radiation AI (UVAI; 340-400 nm). Exposure to UVAI induced 1.6 +/- 0.08 x 10(-6) (mean +/- standard error of mean) pyrimidine dimers per 10(8) Da of DNA per J/m2. Edema in irradiated animals was determined as an increase in skinfold thickness. A dose of 1.8 x 10(6) J/m2 of UVAI that resulted in a 50% increase in skinfold thickness (SFT50%) would have induced 1.0 x 10(5) dimers per basal cell genome. A similar increase in SFT induced by full spectrum solar ultraviolet radiation (290-400 nm) would accompany the induction of 11.0 x 10(5) pyrimidine dimers per basal cell genome. These results support a hypothesis that UVAI-induced pathological changes of the skin are mediated through the formation of nondimer photoproducts.
Ley
R D
RD
Department of Cell Biology and Physiology and the Cancer Research and Treatment Center, University of New Mexico Health Sciences Center, Albuquerque, NM 87131-5218, USA.
Fourtanier
A
A
D000818:Animals; D004247:DNA / Q000378:metabolism / Q000528:radiation effects; D004249:DNA Damage; D004487:Edema / Q000209:etiology* / Q000378:metabolism; D005260:Female; D051379:Mice; D008812:Mice, Hairless; D011740:Pyrimidine Dimers / Q000096:biosynthesis* / Q000528:radiation effects; D011833:Radiation Injuries, Experimental / Q000209:etiology / Q000378:metabolism; D012867:Skin / Q000378:metabolism / Q000528:radiation effects*; D012871:Skin Diseases / Q000209:etiology* / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects*
D011740:Pyrimidine Dimers; D004247:DNA