title: Purification, crystallization and preliminary diffraction study of beta-galactosidase from Penicillium sp.
abstract: Crystals of an extracellular beta-galactosidase from Penicillium sp. (MW = 120 +/- 5 kDa) have been obtained from a sodium phosphate buffer using PEG as precipitant. The crystals belong to the tetragonal space group P4(1)or P4(3), with unit-cell parameters a = b = 110.82, c = 161.28 A, and diffract to 1.85 A resolution at a synchrotron source.
authors.affiliation: Petersburg Nuclear Physics Institute, Gatchina, St Petersburg, 188350, Russia.
authors.forename: K N
authors.initials: KN
authors.lastname: Neustroev
authors.forename: E A
authors.initials: EA
authors.lastname: de Sousa
authors.forename: A M
authors.initials: AM
authors.lastname: Golubev
authors.lastname: Brandão Neto
authors.forename: E V
authors.initials: EV
authors.lastname: Eneyskaya
authors.forename: A A
authors.initials: AA
authors.lastname: Kulminskaya
authors.forename: I
authors.initials: I
authors.lastname: Polikarpov
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D006031:Glycosylation; D010407:Penicillium / Q000201:enzymology*; D011487:Protein Conformation; D001616:beta-Galactosidase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D001616:beta-Galactosidase