title: Interspecies differences in P-glycoprotein mediated activity of multixenobiotic resistance mechanism in several marine and freshwater invertebrates.
abstract: The presence and function of the P-glycoprotein mediated multixenobiotic resistance (MXR) mechanism was demonstrated in numerous aquatic organisms. The aim of this study was to investigate whether in aquatic organisms exists the inherent, species-specific basal level of MXR activity. Here the results of the direct comparison of the basal (noninduced) level of MXR activity measured in several marine (Mytilus galloprovincialis, Monodonta turbinata, Patella lusitanica) and freshwater (Dreissena polymorpha, Viviparus viviparus, Anodonta cygnea) molluscs species are presented. The primary criterion for the assessment and quantification of the basal level of MXR activity was the ratio (R) between the accumulation or efflux of the fluorescent model MXR substrates (rhodamine B or rhodamine 123) in or from the gills, measured with and in the absence of model MXR inhibitors verapamil or cyclosporin A. Significantly different levels of MXR activity were found in the species investigated. These levels generally show a relatively good correlation with the level of pollution present in their natural habitats. Considering these results a conclusion was reached that in aquatic organisms indeed exist the different inherent, species-specific levels of MXR activity. The identified levels might be, at least partly, responsible either for the resistance to, or for the sensitivity of a particular species to organic pollution.
authors.affiliation: Department for Marine and Environmental Research, Rudjer Bosković Institute, Zagreb, Croatia. smital@rudjer.irb.hr
authors.forename: T
authors.initials: T
authors.lastname: Smital
authors.forename: R
authors.initials: R
authors.lastname: Sauerborn
authors.forename: B
authors.initials: B
authors.lastname: Pivcević
authors.forename: S
authors.initials: S
authors.lastname: Krca
authors.forename: B
authors.initials: B
authors.lastname: Kurelec
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000502:physiology*; D018528:ATP-Binding Cassette Transporters / Q000502:physiology; D000818:Animals; D049872:Bivalvia; D016572:Cyclosporine / Q000494:pharmacology; D008974:Mollusca; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D013045:Species Specificity; D014700:Verapamil / Q000494:pharmacology; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015262:Xenobiotics / Q000633:toxicity*
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D018528:ATP-Binding Cassette Transporters; D016572:Cyclosporine; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D014700:Verapamil; D014874:Water Pollutants, Chemical; D015262:Xenobiotics