Inhibition of human peripheral blood neutrophil respiratory burst by alcohol-based venipuncture site disinfection.
Ethanol inhibits the respiratory burst of neutrophils. Therefore, the effects of alcohol-based skin disinfection on oxygen metabolism in neutrophils were tested using 70% ethanol or an ethanol-isopropanol-n-propanol mixture. Neutrophil respiratory burst activity as assessed fluorometrically by oxidation of 2', 7'-dichlorofluorescein diacetate increased at 10 min after disinfection with 70% ethanol compared to the activity at 30 s. The increase was significant for triggering oxidative burst with formyl-peptide but not with phorbol myristate acetate.
Reinisch
N
N
Section of Internal Medicine and Nephrology, Department of Internal Medicine and Therapeutics, University of Pavia, I-27100 Pavia, Italy.
Wiedermann
C J
CJ
Ricevuti
G
G
D000433:1-Propanol / Q000009:adverse effects; D019840:2-Propanol / Q000009:adverse effects; D000438:Alcohols / Q000009:adverse effects*; D004202:Disinfectants / Q000009:adverse effects*; D000431:Ethanol / Q000009:adverse effects; D005452:Fluoresceins; D006801:Humans; D066298:In Vitro Techniques; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism*; D018962:Phlebotomy / Q000009:adverse effects; D016897:Respiratory Burst / Q000187:drug effects*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000438:Alcohols; D004202:Disinfectants; D005452:Fluoresceins; C029569:diacetyldichlorofluorescein; D000431:Ethanol; D009240:N-Formylmethionine Leucyl-Phenylalanine; D000433:1-Propanol; D019840:2-Propanol; D013755:Tetradecanoylphorbol Acetate