Oxidative stress, heat shock and drought differentially affect expression of a tobacco protein phosphatase 2C.
A protein phosphatase 2C (PP2C)-homologous cDNA was isolated from Nicotiana tabacum (NtPP2C1). The deduced protein sequence of 416 amino acids showed the highest degree of similarity to the PP2C of Arabidopsis thaliana (AtPP2CA) implicated in abscisic acid signalling. The expression of NtPP2C1 was strongly induced by drought, but repressed by oxidative stress and heat shock. It is suggested that NtPP2C1 operates at the junction of drought, heat shock and oxidative stress.
Vranová
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E
Vakgroep Moleculaire Genetica and Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie (VIB), Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium.
Langebartels
C
C
Van Montagu
M
M
Inzé
D
D
Van Camp
W
W
D000595:Amino Acid Sequence; D018076:DNA, Complementary; D004190:Disasters; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D018869:Heat-Shock Response; D008969:Molecular Sequence Data; D018384:Oxidative Stress; D010749:Phosphoprotein Phosphatases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010947:Plants, Toxic; D054648:Protein Phosphatase 2; D000071636:Protein Phosphatase 2C; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000201:enzymology*; D014158:Transcription, Genetic; D014867:Water
D018076:DNA, Complementary; D029701:Saccharomyces cerevisiae Proteins; D014867:Water; C087358:PTC1 protein, S cerevisiae; D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2; D000071636:Protein Phosphatase 2C