Developmental patterns of mST3GalV mRNA expression in the mouse: in situ hybridization using DIG-labeled RNA probes.
mST3GalV synthesizes ganglioside GM3, the precursor for simple and complex a- and b- series gangliosides, and the expression and regulation of mST3GalV (CMP-NeuAc: lactosylceramide alpha2,3-sialyltransferase) activity is central to the production of almost all gangliosides, a class of glycosphingolipids implicated in variety of cellular processes such as transmembrane signaling, synaptic transmission, specialized membrane domain formation and cell-cell interactions. To understand the developmental expression of mST3GalV in mice, we investigated the spatial and temporal expression of mST3GalV mRNA during the mouse embryogenesis [embryonic (E) days; E9, E11, E13, E15] by in situ hybridization with digoxigenin-labeled RNA probes. All tissues from E9 and E11 were positive for mST3GalV mRNA. On E13, mST3GalV mRNA was expressed in various neural and non-neural tissues. In contrast to these, on E15, the telencephalon and liver produced a strong expression of mST3Gal V which was a quite similar to that of E13. In this stage, mST3GalV mRNA was also expressed in some non-neural tissues. These data indicate that mST3GalV is differently expressed at developmental stages of embryo, and this may be importantly related with regulation of organogenesis in mice.
Ji
M Y
MY
Division of Biological Science, College of Natural Sciences, WonKwang University, Iksan, Jeonbuk, Korea.
Lee
Y C
YC
Do
S
S
Nam
S Y
SY
Jung
K Y
KY
Kim
H M
HM
Park
L K
LK
Choo
Y K
YK
D000818:Animals; D005260:Female; D005679:G(M3) Ganglioside / Q000096:biosynthesis*; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D017403:In Situ Hybridization; D051379:Mice; D011247:Pregnancy; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis*; D012799:Sialyltransferases / Q000235:genetics*
D005679:G(M3) Ganglioside; D015347:RNA Probes; D012333:RNA, Messenger; D012799:Sialyltransferases