EIU in the rat promotes the potential of syngeneic retinal cells injected into the vitreous cavity to induce PVR.
OBJECTIVE
To determine whether syngeneic retinal cells injected in the vitreous cavity of the rat are able to initiate a proliferative process and whether the ocular inflammation induced in rats by lipopolysaccharide (LPS) promotes this proliferative vitreoretinopathy (PVR).


METHODS
Primary cultured differentiated retinal Müller glial (RMG) and retinal pigmented epithelial (RPE) cells isolated from 8 to 12 postnatal Lewis rats were injected into the vitreous cavity of 8- to 10-week-old Lewis rats (10(5) cells/eye in 2 microlieter sterile saline), with or without the systemic injection of 150 microgram LPS to cause endotoxin-induced uveitis (EIU). Control groups received an intravitreal injection of 2 microliter saline. At 5, 15, and 28 days after cell injections, PVR was clinically quantified, and immunohistochemistry for OX42, ED1, vimentin (VIM), glial fibrillary acidic protein (GFAP), and cytokeratin was performed.


RESULTS
The injection of RMG cells, alone or in combination with RPE cells, induced the preretinal proliferation of a GFAP-positive tissue, that was enhanced by the systemic injection of LPS. Indeed, when EIU was induced at the time of RMG cell injection into the vitreous cavity, the proliferation led to retinal folds and localized tractional detachments. In contrast, PVR enhanced the infiltration of inflammatory cells in the anterior segment of the eye.


CONCLUSIONS
In the rat, syngeneic retinal cells of glial origin induce PVR that is enhanced by the coinduction of EIU. In return, vitreoretinal glial proliferation enhanced the intensity and duration of EIU.
Behar-Cohen
F F
FF
Department of Ophthalmology of Hôtel-Dieu of Paris Hospital. Institut National de la Santé et de la Recherche Médicale, Paris, France. beharcohen@aol.com
Thillaye-Goldenberg
B
B
de Bizemont
T
T
Savoldelli
M
M
Chauvaud
D
D
de Kozak
Y
Y
D000818:Animals; D017690:Cell Transplantation; D002478:Cells, Cultured; D019084:Fluorescent Antibody Technique, Indirect; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007267:Injections; D007633:Keratins / Q000378:metabolism; D008070:Lipopolysaccharides; D009457:Neuroglia / Q000378:metabolism / Q000637:transplantation*; D010857:Pigment Epithelium of Eye / Q000378:metabolism / Q000637:transplantation*; D051381:Rats; D011917:Rats, Inbred Lew; D017463:Receptors, Complement 3b / Q000378:metabolism; D012160:Retina / Q000378:metabolism / Q000637:transplantation*; D012163:Retinal Detachment / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D012486:Salmonella typhimurium; D014185:Transplantation, Isogeneic; D014605:Uveitis / Q000150:complications* / Q000378:metabolism / Q000473:pathology; D014746:Vimentin / Q000378:metabolism; D018630:Vitreoretinopathy, Proliferative / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D014822:Vitreous Body / Q000601:surgery*
D005904:Glial Fibrillary Acidic Protein; D008070:Lipopolysaccharides; D017463:Receptors, Complement 3b; D014746:Vimentin; D007633:Keratins