Downregulation of KRC induces proliferation, anchorage independence, and mitotic cell death in HeLa cells.
The large zinc finger protein KRC regulates transcription of target genes via the kappaB gene enhancer element. As an attempt to investigate the cellular function of KRC, we have established cell lines stably transfected with KRC expression vectors. Introduction of a vector directing expression of a transcript antisense to KRC mRNAs in several mammalian cell lines resulted in accelerated proliferation. Furthermore, in HeLa cells, downregulation of KRC conferred anchorage-independent growth and promoted cell cycle progression without an intervening cytokinesis, culminating in the formation of multinucleated giant cells. Ultimately these cells died.
Allen
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Program of Molecular, Cellular, and Developmental Biology, Ohio State University, Columbus, Ohio, 43210, USA.
Wu
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D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002467:Cell Nucleus; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D015536:Down-Regulation; D015870:Gene Expression; D015726:Giant Cells; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008938:Mitosis / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D014162:Transfection; D016335:Zinc Fingers
D004268:DNA-Binding Proteins; C084779:Hivep3 protein, mouse; D014157:Transcription Factors