Analysing uncharted transcriptomes with SAGE.
Methods of comprehensive gene expression analysis have traditionally been limited to analysing bulk tissue or millions of cells. New modifications of serial analysis of gene expression (SAGE) have now permitted the analysis of gene expression in cell subpopulations or microanatomic structures, providing access to unexplored transcriptomes of normal and disease biology.
Velculescu
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Johns Hopkins Oncology Center, Johns Hopkins University School of Medicine, 1650 Orleans St., Baltimore MD 21231, USA. velculescu@jhmi.edu
Vogelstein
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B
Kinzler
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KW
D000818:Animals; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D016208:Databases, Factual; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D006801:Humans; D020407:Internet; D008832:Microchemistry / Q000379:methods; D008979:Moloney murine leukemia virus / Q000201:enzymology; D009369:Neoplasms / Q000235:genetics; D016133:Polymerase Chain Reaction; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D018076:DNA, Complementary; D004273:DNA, Neoplasm; D012194:RNA-Directed DNA Polymerase