Expression of cell volume-regulated kinase h-sgk in pancreatic tissue.
Transcript levels of the human serine/threonine kinase h-sgk have been found to be highest in pancreas. In the present study, localization and regulation of h-sgk transcription in pancreatic tissue were elucidated. As was apparent from radioactive in situ hybridization, most pancreatic acinar cells expressed high levels of h-sgk mRNA. h-sgk mRNA-positive cells were also found in ductal epithelia but not in pancreatic islets. In biopsy specimens from patients with pancreatitis, h-sgk mRNA levels were decreased in acinar cells but abundant in numerous mononuclear interstitial cells within areas of pancreatic necrosis and fibrosis. As shown by Northern blotting, h-sgk transcription in DAN-G pancreatic tumor cells is upregulated by osmotic cell shrinkage, serum, phorbol esters (phorbol 12,13-didecanoate), and Ca(2+) ionophore A-23187 and decreased by staurosporine and cAMP. In conclusion, h-sgk transcription is regulated not only by cell volume but also by serum, protein kinase C stimulation, cAMP, and increase of intracellular Ca(2+) activity. The kinase may participate not only in normal function of exocrine pancreas but also in fibrosing pancreatitis.
Klingel
K
K
Department of Molecular Pathology, Institute of Pathology, University of Tübingen, D-72076, Tübingen, Germany.
Wärntges
S
S
Bock
J
J
Wagner
C A
CA
Sauter
M
M
Waldegger
S
S
Kandolf
R
R
Lang
F
F
D000208:Acute Disease; D015152:Blotting, Northern; D048429:Cell Size / Q000502:physiology; D002908:Chronic Disease; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D017874:Immediate-Early Proteins; D017403:In Situ Hybridization; D008264:Macrophages / Q000502:physiology; D009687:Nuclear Proteins; D010183:Pancreatic Ducts / Q000166:cytology* / Q000201:enzymology*; D010190:Pancreatic Neoplasms; D010195:Pancreatitis / Q000378:metabolism / Q000473:pathology; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D015533:Transcriptional Activation / Q000502:physiology; D014407:Tumor Cells, Cultured; D000099184:Serum-Glucocorticoid Regulated Kinases
D017874:Immediate-Early Proteins; D009687:Nuclear Proteins; D017346:Protein Serine-Threonine Kinases; D012333:RNA, Messenger; D000099184:Serum-Glucocorticoid Regulated Kinases