Regulation of elongation growth by gibberellin in root segments of Lemna minor.
Hormonal control of elongation growth was analyzed in segments excised from the elongation zone of Lemna roots. Exogenous GA3 did not promote the segment elongation but rather inhibited it. Uniconazole-P, a gibberellin biosynthesis inhibitor, significantly inhibited the segment elongation, and the inhibitory effect was completely nullified by GA3. In the epidermis, cell elongation was inhibited, but lateral cell expansion was not affected by uniconazole-P. Orientation of cortical microtubules of epidermal cells was disturbed by treatment with uniconazole-P for 12 h, and the disorganization of cortical microtubules was ameliorated by GA3. These findings suggested that disorganization of cortical microtubules induced inhibition of elongation growth of root. However, stabilization of cortical microtubules by taxol, a microtubule-stabilizing agent, did not affect the inhibition of segment elongation by uniconazole-P. These results suggested that endogenous gibberellin controls the elongation growth of root by regulating cell elongation.
Inada
S
S
Department of Life Science, Faculty of Science, Himeji Institute of Technology, Harima Science Park City, Hyogo, Japan. inada@sci.himeji-tech.ac.jp
Shimmen
T
T
D048429:Cell Size / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005875:Gibberellins / Q000494:pharmacology*; D007210:Indoleacetic Acids / Q000494:pharmacology; D019684:Magnoliopsida; D008870:Microtubules / Q000187:drug effects; D017239:Paclitaxel / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018517:Plant Roots / Q000187:drug effects* / Q000254:growth & development; D014230:Triazoles / Q000494:pharmacology
D005875:Gibberellins; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D014230:Triazoles; C030737:indoleacetic acid; C007842:gibberellic acid; D017239:Paclitaxel; C092705:uniconazole