Structural and expression analysis of uricase mRNA from Lotus japonicus.
Uricase (nodulin-35) cDNA, LjUr, was isolated from nodules of a model legume, Lotus japonicus. LjUr expression was most abundant in nodules, although it was detected in nonsymbiotic tissues as well, particularly in roots. Expression in nodules was detected in uninfected cells, nodule parenchyma, and, more intensely, in vascular bundles. Phylogenetic analysis of uricase sequences from various legumes indicated that uricases of amide- and ureide-transporting legumes form two distinct clades. LjUr is in the cluster of amide-transport legumes even though L. japonicus bears determinate nodules.
Takane
K
K
Department of Plant Physiology, National Institute of Agrobiological Resources, Tsukuba, Ibaraki, Japan. takane@abr.affrc.go.jp
Tajima
S
S
Kouchi
H
H
D000595:Amino Acid Sequence; D005091:Exons; D007887:Fabaceae / Q000145:classification / Q000201:enzymology* / Q000235:genetics*; D015870:Gene Expression; D015723:Gene Library; D017343:Genes, Plant; D008969:Molecular Sequence Data; D020675:Peroxisomes / Q000201:enzymology; D010802:Phylogeny; D018517:Plant Roots / Q000201:enzymology; D018514:Plant Structures / Q000201:enzymology; D010946:Plants, Medicinal; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D018749:RNA, Plant / Q000032:analysis / Q000235:genetics; D014503:Urate Oxidase / Q000737:chemistry / Q000235:genetics*
D012333:RNA, Messenger; D018749:RNA, Plant; D014503:Urate Oxidase