Trypanosoma cruzi: infectivity of clonal genotype infections in acute and chronic phases in mice.
Eight Trypanosoma cruzi stocks pertaining to the clonal genotypes 19/20, 32, and 39 have been characterized for three experimental parameters of infectivity in Balb/c mice: (i) percentage of mice with a patent parasitemia (% MPP), (ii) maximum parasitemia (MP), and (iii) percentage of mice with positive hemoculture (% MPH). By order of decreasing values, the values recorded for the clonal genotypes ranked as follows: 19/20, 32, and 39, except for the % MPP parameter, for which 19/20 and 32 were not statistically different. The rate of successful reisolation after infection in mice, analyzed by multilocus enzyme electrophoresis and random amplified polymorphic DNA typing, was statistically different according to the clonal genotype and was different for uniclonal infections and for mixed infections by two different clonal genotypes. These results confirm that T. cruzi clonal genotypes differ significantly in their infectivity in mice.
de Lana
M
M
Centre d'Etudes sur le Polymorphisme des Microorganismes (CEPM), Unité Mixte de Recherche no. 9926 Centre National de la Recherche Scientifique (CNRS)/Institut de Recherche pour le Développement (IRD), B.P. 5045, 34032 Montpellier Cedex 1, France.
da Silviera Pinto
A
A
Bastrenta
B
B
Barnabé
C
C
Noël
S
S
Tibayrenc
M
M
D000208:Acute Disease; D000818:Animals; D014355:Chagas Disease / Q000469:parasitology*; D016009:Chi-Square Distribution; D002908:Chronic Disease; D004586:Electrophoresis; D005819:Genetic Markers; D005838:Genotype; D007527:Isoenzymes / Q000032:analysis / Q000235:genetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018512:Parasitemia / Q000469:parasitology; D019105:Random Amplified Polymorphic DNA Technique; D014349:Trypanosoma cruzi / Q000145:classification / Q000235:genetics / Q000472:pathogenicity*
D005819:Genetic Markers; D007527:Isoenzymes