Susceptibility of Biomphalaria tenagophila and Biomphalaria straminea to Schistosoma mansoni infection detected by low stringency polymerase chain reaction.
In order to determine Schistosoma mansoni infection rates in Biomphalaria tenagophila and B. straminea, low stringency polymerase chain reaction (LS-PCR) technique was used as a complementary method to light exposure technique. LS-PCR has already been standardized in our laboratory to detect the trematode DNA in B. glabrata. Higher S. mansoni infection rates were detected using conventional method and LS-PCR. The parasite DNA profile was detected in both species after 7-day exposure to miracidia, using LS-PCR. This technique enables early detection of schistosomiasis transmission focuses, in endemic areas, before the beginning of cercariae shedding.
Jannotti-Passos
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LK
Centro de Pesquisas René Rachou, FIOCRUZ, Laboratório de Malacologia, Belo Horizonte, MG, Brasil.
de Souza
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CP
D000818:Animals; D001700:Biomphalaria / Q000469:parasitology*; D006790:Host-Parasite Interactions; D008027:Light; D016133:Polymerase Chain Reaction / Q000379:methods*; D012550:Schistosoma mansoni / Q000302:isolation & purification / Q000502:physiology*