[Antigen strategy: a new way for gene therapy].
Synthetic purine-rich or pyridine-rich oligonucleotides have been shown to form stable triplex by binding to specific homopurine-homopyridine sequences in double-stranded DNA. Several studies have suggested that triplex formation in the transcription-regulated regions of genes could inhibit their transcription in vitro or in vivo. This new technology was called antigene strategy, which would be of great value in the treatment of cancer and virus infection. However, the low stability of triplex and restricting target selection of homopurine-homopyridine tracts in selected genes greatly reduced its utility in antigen strategies. Further work is still needed.
Liu
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DX
Insititute of Molecular Biology, First Military Medical University, Guang Zhou.
Wang
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D004247:DNA / Q000494:pharmacology*; D016259:Genes, myc / Q000235:genetics; D015316:Genetic Therapy; D006801:Humans; D009369:Neoplasms / Q000628:therapy; D009690:Nucleic Acid Conformation; D014158:Transcription, Genetic / Q000187:drug effects
C051802:triplex DNA; D004247:DNA