title: Nucleotide sequence of the chromosomal ampC gene of Enterobacter aerogenes.
abstract: The AmpC beta-lactamase gene and a small portion of the regulatory ampR sequence of Enterobacter aerogenes 97B were cloned and sequenced. The beta-lactamase had an isoelectric point of 8 and conferred cephalosporin and cephamycin resistance on the host. The sequence of the cloned gene is most closely related to those of the ampC genes of E. cloacae and C. freundii.
authors.affiliation: Department of Pathology and Laboratory Medicine, Albany Medical Center Hospital, Albany, New York 12208, USA.
authors.forename: K E
authors.initials: KE
authors.lastname: Preston
authors.forename: C C
authors.initials: CC
authors.lastname: Radomski
authors.forename: R A
authors.initials: RA
authors.lastname: Venezia
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D002511:Cephalosporins / Q000494:pharmacology; D002513:Cephamycins / Q000494:pharmacology; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000032:analysis; D004352:Drug Resistance, Microbial; D021902:Enterobacter aerogenes / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D007525:Isoelectric Focusing; D008969:Molecular Sequence Data; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D001618:beta-Lactamases / Q000737:chemistry / Q000145:classification / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D002511:Cephalosporins; D002513:Cephamycins; D004269:DNA, Bacterial; C048795:AmpC beta-lactamases; D001618:beta-Lactamases