title: Odorants stimulate the ERK/mitogen-activated protein kinase pathway and activate cAMP-response element-mediated transcription in olfactory sensory neurons.
abstract: Olfactory sensory neurons (OSNs) respond acutely to volatile molecules and exhibit adaptive responses including desensitization to odorant exposure. Although mechanisms for short term adaptation have been described, there is little evidence that odorants cause long lasting, transcription-dependent changes in OSNs. Here we report that odorants stimulate cAMP-response element (CRE)-mediated transcription in OSNs through Ca2+ activation of the ERK/MAPK/p90rsk pathway. Odorant stimulation of ERK phosphorylation was ablated by inhibition of calmodulin-dependent protein kinase II suggesting that odorant activation of ERK is mediated through this kinase. Moreover, a brief exposure in vivo to an odorant in vapor phase stimulated CRE-mediated gene transcription in discrete populations of OSNs. These data suggest that like central nervous system neurons, OSNs may undergo long term adaptive changes mediated through CRE-mediated transcription.
authors.affiliation: Department of Pharmacology, University of Washington, Seattle, Washington 98195-7280, USA.
authors.forename: W C
authors.initials: WC
authors.lastname: Watt
authors.forename: D R
authors.initials: DR
authors.lastname: Storm
mesh_terms: D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D007700:Kinetics; D020935:MAP Kinase Signaling System; D009475:Neurons, Afferent / Q000201:enzymology / Q000378:metabolism*; D009812:Odorants; D009833:Olfactory Pathways / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D051381:Rats; D014158:Transcription, Genetic
chemical_list: D000242:Cyclic AMP