[The effect of glucoprotein component of musk on arachidonic acid metabolizing enzymes in rat polymorphonuclear leukocytes].
To investigate the effects of musk-1, a glucoprotein component isolated from the water extract of musk, on arachidonic acid metabolizing enzymes, which include phospholipase A2(PLA2), 5-lipoxygenase (5-LO), and cyclooxygenase (COX), in rat polymorphonuclear leukocytes, an in vitro incubation system with rat polymorphonuclear leukocytes or homogenate supernatant of the same cells was used. In comparison with control, musk-1 at final concentrations of 1-100 micrograms/ml can increase AA release from PMNL by 6.0%-21.6%, decrease LTB4 biosynthesis in homogenate supernatant of the cells by 9%-81%, but increase 6-keto-PGF1 alpha production by 18.2%-85.4%.
Wang
W
W
Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing.
Bai
J
J
Cheng
G
G
Zhu
X
X
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis; D000818:Animals; D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism*; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D007975:Leukotriene B4 / Q000096:biosynthesis; D008297:Male; D009504:Neutrophils / Q000201:enzymology*; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D005229:Fatty Acids, Monounsaturated; C008563:musk; D007975:Leukotriene B4; D015121:6-Ketoprostaglandin F1 alpha; D001094:Arachidonate 5-Lipoxygenase; D011451:Prostaglandin-Endoperoxide Synthases; D010741:Phospholipases A; D054467:Phospholipases A2