[Free fatty acids promoting PTP1B expression in rat skeletal muscle and hepatic cells].
OBJECTIVE
To determine whether that free fatty acids (FFAs) impair glucose metabolism is associated with altered insulin signaling system.


METHODS
After skeletal muscle and hepatic cells were incubated with palmitate (0.25 mmol/L) or oleate (0.125 mmol/L) for 6, 12 or 24 hours, the protein abundance of SH-PTP2 and PTP1B was assessed by western blot.


RESULTS
SH-PTP2 protein levels showed no significant change in both fatty acids treated muscle and hepatic cells at all time points. The PTP1B was significantly elevated in both liver and muscle cells incubated with FFAs at 6, 12 and 24 hours.


CONCLUSIONS
FFAs promote the expression of PTP1B in rat skeletal muscle and liver cells, and the elevated PTP1B may mediate the insulin resistance induced by FFAs.
Shao
J
J
Department of Endocrinology, First Teaching Hospital of Beijing Medical University.
Gao
Y
Y
Yuan
Z
Z
D000818:Animals; D002478:Cells, Cultured; D005227:Fatty Acids / Q000494:pharmacology*; D007333:Insulin Resistance; D047908:Intracellular Signaling Peptides and Proteins; D008099:Liver / Q000166:cytology*; D008297:Male; D018482:Muscle, Skeletal / Q000166:cytology*; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6; D017027:Protein Tyrosine Phosphatases / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D054593:SH2 Domain-Containing Protein Tyrosine Phosphatases; D018909:src Homology Domains
D005227:Fatty Acids; D047908:Intracellular Signaling Peptides and Proteins; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11; D053824:Protein Tyrosine Phosphatase, Non-Receptor Type 6; D017027:Protein Tyrosine Phosphatases; C516610:Ptpn1 protein, rat; C516727:Ptpn11 protein, rat; C509250:Ptpn6 protein, rat; D054593:SH2 Domain-Containing Protein Tyrosine Phosphatases