[Ribonuclease from Bacillus thuringiensis var. subtoxicus. Gene structure and biosynthesis regulation].
The gene for extracellular guanyl-specific ribonuclease of Bacillus thuringiensis var. subtoxicus (RNase Bth), a close homologue of the B. intermedius RNase (binase), was completely sequenced. Analysis of nucleotide sequences in the regions adjoining RNase genes revealed an identical organization of the chromosomal loci of RNase Bth and binase. Growth characteristics of the Bacillus thuringiensis var. subtoxicus strain and its synthesis of RNase were studied. It was shown that the exogenous inorganic phosphate inhibits the biosynthesis of RNase. At the same time, actinomycin D in low doses stimulates the enzyme synthesis. Comparative analysis of the influence of inorganic phosphate and actinomycin D on the biosynthesis of RNAse Bth and binase suggests a possibility of coincidence of regulatory pathways of synthesis of these enzymes.
Shul'ga
A A
AA
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Znamenskaia
L V
LV
Morozova
O V
OV
Leshchinskaia
I B
IB
Kirpichnikov
M P
MP
D000595:Amino Acid Sequence; D001413:Bacillus thuringiensis / Q000201:enzymology* / Q000254:growth & development; D001483:Base Sequence; D003609:Dactinomycin / Q000494:pharmacology; D004722:Endoribonucleases / Q000235:genetics; D008969:Molecular Sequence Data; D010710:Phosphates / Q000494:pharmacology; D012260:Ribonucleases / Q000096:biosynthesis* / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D010710:Phosphates; D003609:Dactinomycin; D004722:Endoribonucleases; D012260:Ribonucleases; C022543:ribonuclease T(2); C415908:Bacillus thuringiensis ribonuclease