Structure of a dioxygen reduction enzyme from Desulfovibrio gigas.
Desulfovibrio gigas is a strict anaerobe that contains a well-characterized metabolic pathway that enables it to survive transient contacts with oxygen. The terminal enzyme in this pathway, rubredoxin:oxygen oxidoreductase (ROO) reduces oxygen to water in a direct and safe way. The 2.5 A resolution crystal structure of ROO shows that each monomer of this homodimeric enzyme consists of a novel combination of two domains, a flavodoxin-like domain and a Zn-beta-lactamase-like domain that contains a di-iron center for dioxygen reduction. This is the first structure of a member of a superfamily of enzymes widespread in strict and facultative anaerobes, indicating its broad physiological significance.
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Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. da República, Apartado 127, 2781-901 Oeiras, Portugal.
Silva
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Gomes
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Matias
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Coelho
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Sieker
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L
Macedo
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S
Liu
M Y
MY
Oliveira
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Teixeira
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M
Xavier
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AV
Rodrigues-Pousada
C
C
Carrondo
M A
MA
Le Gall
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J
D000595:Amino Acid Sequence; D001665:Binding Sites; D018360:Crystallography, X-Ray; D003901:Desulfovibrio / Q000201:enzymology* / Q000235:genetics; D019281:Dimerization; D005418:Flavodoxin / Q000737:chemistry; D007501:Iron / Q000378:metabolism; D008024:Ligands; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D014867:Water / Q000378:metabolism; D001618:beta-Lactamases / Q000737:chemistry
D005418:Flavodoxin; D008024:Ligands; D011994:Recombinant Proteins; D014867:Water; D007501:Iron; D010088:Oxidoreductases; C081469:rubredoxin-oxygen oxidoreductase; D001618:beta-Lactamases; D010100:Oxygen