Plasma, urinary, and salivary 8-epi-prostaglandin f2alpha levels in normotensive and preeclamptic pregnancies.
OBJECTIVE
Our purpose was to measure and compare plasma, urinary, and salivary concentrations of 8-epi-prostaglandin F(2alpha) (8-isoprostane) in women with normotensive pregnancies and the respective concentrations in pregnancies complicated by preeclampsia.


METHODS
Plasma, urinary, and salivary 8-isoprostane levels were measured in pregnant women with preeclampsia (n = 40), normotensive pregnant women (n = 20), and nonpregnant women (n = 10). One-way analysis of variance was used to determine significant differences.


RESULTS
Plasma free 8-isoprostane concentrations were increased in women with severe preeclampsia (342 +/- 50 pg/mL), in comparison with nonpregnant women (129 +/- 17 pg/mL) and normotensive pregnant women (150 +/- 11 pg/mL; P =.003, and.0001, respectively). Urinary excretion of 8-isoprostane was slightly but not significantly decreased in preeclampsia (1200 +/- 227 pg/mL), in comparison with urinary excretion in nonpregnant women (1625 +/- 364 pg/mL) and normotensive pregnant women (2149 +/- 432 pg/mL). Salivary concentrations of 8-isoprostane were increased in normotensive women (496 +/- 113 pg/mL), in comparison with nonpregnant women (150 +/- 27 pg/mL) but were not related to preeclampsia (419 +/- 96 pg/mL; P </=.003).


CONCLUSIONS
Free 8-isoprostane concentrations are increased in the plasma of women with severe preeclampsia. Further studies are warranted to determine whether such increases are caused by an increase in phospholipase A(2) activity, or by lipid peroxidation, or by a decrease in renal excretion.
McKinney
E T
ET
Department of Obstetrics and Gynecology, The University of Tennessee, Memphis, TN, USA.
Shouri
R
R
Hunt
R S
RS
Ahokas
R A
RA
Sibai
B M
BM
D001794:Blood Pressure; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000097:blood* / Q000378:metabolism* / Q000652:urine; D028441:F2-Isoprostanes; D005260:Female; D006801:Humans; D011225:Pre-Eclampsia / Q000097:blood / Q000378:metabolism* / Q000652:urine; D011247:Pregnancy / Q000097:blood / Q000378:metabolism* / Q000652:urine; D012016:Reference Values; D012463:Saliva / Q000378:metabolism*
D028441:F2-Isoprostanes; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost