Tissue repair with a therapeutic transcription factor.
The healing of tissue involves a wide range of molecular, cellular, and physiological events that are coordinated in a temporally specific manner. The cellular transcription factor early growth response factor 1 (Egr-1) is expressed minutes after acute injury and serves to stimulate the production of a class of growth factors whose role is to promote tissue repair. We have studied the effects of Egr-1 expression at the site of dermal wounding in rodents. We find that Egr-1 promotes angiogenesis in vitro and in vivo, increases collagen production, and accelerates wound closure. These results show that Egr-1 gene therapy accelerates the normal healing process and raises the potential use of this therapeutic transcription factor for any aspect of tissue repair.
Bryant
M
M
Wound Healing and Tissue Regeneration Program, Endothelial Gene Expression Group, Vascular Diseases Unit, Glaxo-Wellcome Medicines Research Centre, Stevenage, Hertfordshire, SG1 2NY, UK.
Drew
G M
GM
Houston
P
P
Hissey
P
P
Campbell
C J
CJ
Braddock
M
M
D000818:Animals; D019470:Biolistics; D003094:Collagen / Q000096:biosynthesis; D018076:DNA, Complementary / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics*; D051766:Early Growth Response Protein 1; D016228:Endothelial Growth Factors / Q000096:biosynthesis; D005455:Fluorescent Antibody Technique; D007091:Image Processing, Computer-Assisted; D017874:Immediate-Early Proteins; D007150:Immunohistochemistry; D008222:Lymphokines / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D018919:Neovascularization, Physiologic / Q000235:genetics; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000378:metabolism; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics*; D016212:Transforming Growth Factor beta / Q000096:biosynthesis; D053773:Transforming Growth Factor beta1; D015854:Up-Regulation; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D014945:Wound Healing; D001616:beta-Galactosidase / Q000378:metabolism
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D051766:Early Growth Response Protein 1; C486109:Egr1 protein, mouse; C486110:Egr1 protein, rat; D016228:Endothelial Growth Factors; D017874:Immediate-Early Proteins; D008222:Lymphokines; D010982:Platelet-Derived Growth Factor; C508961:Tgfb1 protein, mouse; C508962:Tgfb1 protein, rat; D014157:Transcription Factors; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; C075278:platelet-derived growth factor A; D003094:Collagen; D001616:beta-Galactosidase