Determination of H(2)S solubility via the reaction with ferric hemoglobin I from the bivalve mollusc Lucina pectinata.
A new, simple and fast spectrophotometric method for the determination of the H(2)S concentration is reported. This method, based on the 1:1 reaction between H(2)S and the ferric derivative of hemoglobin I (HbI) from the bivalve mollusc Lucina pectinata, allows the quantitative determination of H(2)S dissolved in a given solution even at concentrations as low as 1 x 10(-6) M. Note that L. pectinata HbI is considered the physiological receptor of H(2)S.
Boffi
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CNR, Centro di Biologia Molecolare and Dipartimento di Scienze Biochimiche 'Alessandro Rossi Fanelli', Università di Roma 'La Sapienza', Rome, Italy.
Rizzi
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M
Monacelli
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F
Ascenzi
P
P
D000818:Animals; D049872:Bivalvia; D006454:Hemoglobins; D006455:Hemoglobins, Abnormal / Q000737:chemistry* / Q000302:isolation & purification; D006862:Hydrogen Sulfide / Q000032:analysis* / Q000737:chemistry; D007700:Kinetics; D012995:Solubility; D013053:Spectrophotometry; D013816:Thermodynamics
D006454:Hemoglobins; D006455:Hemoglobins, Abnormal; C026236:hemoglobin I, non-mammalian; D006862:Hydrogen Sulfide