DNA fingerprinting of thermophilic lactic acid bacteria using repetitive sequence-based polymerase chain reaction.
DNA fingerprints of lactic acid bacteria were generated by polymerase chain reaction using a primer based on the repetitive elements found in the genome of Streptococcus pneumoniae (BOX-PCR). The method made it possible to identify 37 isolates from raw milk. industrial starters and yogurt. Differentiation at species, subspecies and strain level was possible for Lactobacillus delbrueckii subsp. lactis, Lb. delbrueckii subsp bulgaricus and Str. thermophilus. BOX-PCR was also applied to studying the strain composition of a starter culture and the direct detection of strains in commercial fermented milk.
De Urraza
P J
PJ
Centro de Investigación y Desarrollo en Criotecnología de Alimentos, Facultad de Ciencias Exactas, UNLP-CONICET, La Plata, Argentina.
Gómez-Zavaglia
A
A
Lozano
M E
ME
Romanowski
V
V
De Antoni
G L
GL
D000818:Animals; D001483:Base Sequence; D016172:DNA Fingerprinting / Q000379:methods*; D004269:DNA, Bacterial; D007778:Lactobacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008892:Milk / Q000382:microbiology; D008969:Molecular Sequence Data; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid; D013291:Streptococcus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D015014:Yogurt / Q000382:microbiology
D004269:DNA, Bacterial