Construction of the recombinant retrovirus vector of HBV-S gene and it's expression in eukaryotic cells.
OBJECTIVE
To investigate the effectiveness of recombinant retrovirus vector in gene therapy.


METHODS
The retroviral vector PLXSN-S was constructed and transferred into PA317 by means of electroporation, then HepG(2), P815, and EL4 cells were infected with the pseudovirus produced from PA317, which highly expressed HBsAg. HBsAg expression was tested by RT-PCR and ELISA.


RESULTS
HBsAg was expressed variously in the eukaryotic cells mentioned above. HBsAg (A value) of the cell supernatants (48 h) were 0.92, 0.09, 0.47, respectively.


CONCLUSIONS
The vector used in this study is an effective one to carry genes of interest to target cells and it may be useful in the test for gene therapy.
Zhou
Z
Z
Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China.
Zhang
D
D
Ren
H
H
D002460:Cell Line; D015316:Genetic Therapy; D005822:Genetic Vectors; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D011994:Recombinant Proteins / Q000096:biosynthesis*; D012190:Retroviridae / Q000235:genetics*
D006514:Hepatitis B Surface Antigens; D011994:Recombinant Proteins