[Enhancement of glycine-gated Cl- channel currents by 5-HT and NA in rat sacral dorsal commissural neurons is mediated by protein kinases].
The effects of 5-HT and NA on glycine-gated Cl- channel currents(IGly) and its intracellular mechanisms were investigated in acutely dissociated rat sacral dorsal commissural neurons by using nystatin perforated patch clamp recording. It was found that: (1) the activation of 5-HT2 receptor coupled to IAP-insensitive G-proteins increases intracellular DAG formation through the activation of phospholipase C(PLC). The accumulation of DAG increases the Ca(2+)-independent or novel PKC(nPKC) activity, resulting in the potentiation of IGly; (2) the activation of alpha 2-adrenoceptor by NA coupled to IAP-sensitive G-proteins reduces intracellular cAMP formation through the inhibition of adenylyl cyclase (AC). The reduction of cAMP decreases PKA activity, resulting in the potentiation of IGly.
Xu
T L
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Department of Anatomy, K. K. Leung Brain Research Center, Fourth Military Medical University, Xi'an.
D000818:Animals; D015220:Calcium Channels / Q000187:drug effects; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D009638:Norepinephrine / Q000494:pharmacology*; D011493:Protein Kinase C / Q000502:physiology*; D051381:Rats; D018009:Receptors, Glycine / Q000187:drug effects / Q000502:physiology; D011985:Receptors, Serotonin / Q000187:drug effects / Q000502:physiology; D012701:Serotonin / Q000494:pharmacology*; D013116:Spinal Cord / Q000187:drug effects / Q000502:physiology*
D015220:Calcium Channels; D018009:Receptors, Glycine; D011985:Receptors, Serotonin; D012701:Serotonin; D011493:Protein Kinase C; D009638:Norepinephrine