[Studies on lipase-catalyzed synthesis of L-ascorbyl palmitate in non-aqueous phase].
The investigation on the reaction media and lipases (NOVO435, MML, LIPOLASE, PPL) for enzymatic synthesis of L-ascorbyl palmitate as well as the factors effecting initial rate of synthesis reaction (rotation speed, temperature, water content, enzyme concentration, and substrate concentration) is presented. Among these investigated solvents and lipases, amyl alcohol and NOVO435 is the optimum pair. The reaction conditions have been optimized: 200 r/min, 55 degrees C, water content = 0, the amount of enzyme = 12.5% of the substrate's quantity.
Tang
L H
LH
Wuxi University of Light Industry.
Zhang
H
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D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000096:biosynthesis; D008049:Lipase / Q000378:metabolism*
D008049:Lipase; D001205:Ascorbic Acid; C031226:6-O-palmitoylascorbic acid