Genetic characterization of vanG, a novel vancomycin resistance locus of Enterococcus faecalis.
Enterococcus faecalis strain WCH9 displays a moderate level of resistance to vancomycin (MIC = 16 microgram/ml) and full susceptibility to teicoplanin but is negative by PCR analysis using primers specific for all known enterococcal vancomycin resistance genotypes (vanA, vanB, vanC, vanD, and vanE). We have isolated and sequenced a novel putative vancomycin resistance locus (designated vanG), which contains seven open reading frames, from this strain. These are organized differently from those of all the other enterococcal van loci, and, furthermore, the individual vanG gene products exhibit less than 50% amino acid sequence identity to other van gene products.
McKessar
S J
SJ
Molecular Microbiology Unit, Women's and Children's Hospital, North Adelaide, South Australia 5006, Australia.
Berry
A M
AM
Bell
J M
JM
Turnidge
J D
JD
Paton
J C
JC
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D019729:Carbon-Oxygen Ligases / Q000235:genetics; D004352:Drug Resistance, Microbial; D013293:Enterococcus faecalis / Q000187:drug effects / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010453:Peptide Synthases; D017386:Sequence Homology, Amino Acid; D020713:Vancomycin Resistance / Q000235:genetics*
D001426:Bacterial Proteins; C071437:VanA ligase, Bacteria; C417270:VanG protein, Enterococcus faecalis; D019729:Carbon-Oxygen Ligases; D010453:Peptide Synthases; C122181:VanD protein, Enterococcus faecium