The rat hepatoma cell line H4-II-E-C3 expresses high activities of the high-affinity glutamate transporter GLT-1A.
The rate of uptake of aspartate into the rat hepatoma cell line H4-II-E-C3 is very much higher than that exhibited by normal rat hepatocytes. Using an RT-PCR-based strategy, a glutamate transporter resembling mouse liver GLT-1A has been cloned from H4-II-E-C3 cells. Northern blotting confirmed that relatively high levels of mRNA for GLT-1A are expressed in hepatoma cells compared with negligible levels in rat hepatocytes. To our knowledge, this is the first report of the cloning of a high-affinity glutamate transporter from a transformed cell line and also the first demonstration of functional expression of GLT-1 outside the central nervous system.
Pollard
M
M
Department of Biochemistry, School of Medical Sciences, University Walk, BS8 1TD, Bristol, UK. m.pollard@bristol.ac.uk
McGivan
J
J
D018528:ATP-Binding Cassette Transporters / Q000096:biosynthesis* / Q000235:genetics; D027322:Amino Acid Transport System X-AG; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis; D015870:Gene Expression; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D008969:Molecular Sequence Data; D051381:Rats; D014407:Tumor Cells, Cultured
D018528:ATP-Binding Cassette Transporters; D027322:Amino Acid Transport System X-AG; D018076:DNA, Complementary