Characterisation of two citrus apscaviroids isolated in Spain.
Sequence variability in the PCR amplified cDNAs from two citrus apscaviroid isolates CVd-Ia and CVd-IIId from Spain, was analysed. CVd-IIId sequence was shown to be identical to previously described CVd-III sequences and no important variability was encountered within the viroid population. Conversely, CVd-Ia displayed population heterogeneity as shown by SSCP analysis, Sal I restriction site polymorphism and sequences of 27 CVd-Ia cloned DNAs. The CVd-Ia genomic heterogeneity is characterised by two major subpopulations with the most divergent sequences, and by the presence of individual variants, making a sequence continuum between the two major groups. Most sequence variations are clustered in the left part of the viroid molecule.
Foissac
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Instituto Valenciano de Investigaciones Agrarias, Moncada, Valencia, Spain.
Duran-Vila
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N
D001483:Base Sequence; D002957:Citrus / Q000821:virology*; D018076:DNA, Complementary / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012367:RNA, Viral; D013030:Spain; D014772:Viroids / Q000145:classification / Q000235:genetics*
D018076:DNA, Complementary; D012367:RNA, Viral