Reaction of various lectins to mucin derived from the different layers of rat gastric mucosa: comparison of enzyme-linked lectin binding assay with lectin histochemistry.
The purpose of this study was to demonstrate that the histochemical staining reactivities of lectins in rat stomach actually represent the gastric mucins, and to estimate the utility of the lectins for mucin histochemistry. In this paper, the lectin histochemistry was compared with an enzyme-linked lectin binding assay (ELLA) of the mucins derived from distinct regions and layers of the Sprague-Dawley rat stomach and it was examined to determine the definite binding and problematic binding of the conventional lectin. Among the 10 different biotinylated lectins, Canavalia ensiformis (ConA), Griffonia simplicifolia II (GS-II), Triticuin vulgaris (WGA), Ricinus communis I (RCA-I), Arachis hypogaea (PNA), Glycine max (SBA), Dolichos biflorus (DBA), Ulex europaeus I (UEA-I), Sambucus nigra (SNA) and Maackia amurensis II (MAL-II), examined in this study, GS-II, SBA, DBA, UEA-I, SNA and MAL-II bound clearly to the mucin of distinct regions and layers of the Sprague-Dawley rat stomach in agreement with the results of ELLA. Namely GS-II lectins preferentially bound to the mucin in the mucous neck cells of the corpus area. SBA and DBA clearly recognized the mucin in the covering epithelial mucous cells in the corpus and antral area. UEA-I was widely bound to all the mucin present in both the corpus and antrum. On the other hand, SNA and MAL-II could not react with the mucin obtained from the gastric mucosa but was specifically bound to the mucin purified from the mucous gel layer. These results suggested that the lectins described above are useful histochemical tools to recognize the mucus present in the different regions and layers of Sprague-Dawley rat gastric mucosa.
Kodaira
H
H
Department of Molecular Pharmacology, School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan. kodairah@pharm.kitasato-u.ac.jp
Ishihara
K
K
Hotta
K
K
Kagoshima
M
M
Shimada
H
H
Ishii
K
K
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005753:Gastric Mucosa / Q000737:chemistry*; D006651:Histocytochemistry; D006735:Horseradish Peroxidase; D007202:Indicators and Reagents; D037102:Lectins / Q000737:chemistry*; D008297:Male; D009077:Mucins / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019809:Streptavidin
D007202:Indicators and Reagents; D037102:Lectins; D009077:Mucins; D019809:Streptavidin; D006735:Horseradish Peroxidase