A comparative study on the disintegration of filamentous fungi.
Different methods for cell disintegration were tested for their efficacy on filamentous fungi, including percussion grinding, homogenization using an Ultra-Turrax, chemical treatment and lyophylization. The release of protein from Ganoderma applanatum and Pycnoporus cinnabarinus and the activity of cytoplasmatic glucose-6-phosphate dehydrogenase in the crude extracts were monitored to determine the efficiency of each disintegration technique used. Fungal cells proved to be particularly resistant towards some disintegration methods commonly used for yeasts and bacteria. Best results were obtained using a percussion grinder, if necessary, in combination with an Ultra-Turrax pretreatment.
Taubert
J
J
Institut für Lebensmittelchemie, Universität Hannover, Wunstorfer Strasse 14, 30453, Hannover, Germany.
Krings
U
U
Berger
R G
RG
D001487:Basidiomycota / Q000166:cytology* / Q000201:enzymology; D002458:Cell Fractionation / Q000295:instrumentation / Q000379:methods*; D003600:Cytosol / Q000201:enzymology; D005612:Freeze Drying; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D009172:Mycology / Q000295:instrumentation / Q000379:methods*; D020072:Polyporales / Q000166:cytology* / Q000201:enzymology; D012997:Solvents
D012997:Solvents; D005954:Glucosephosphate Dehydrogenase