Mononuclear cells and cytokines in the cerebrospinal fluid of echovirus 30 meningitis patients.
The local immune response to echovirus 30 meningitis in children was investigated in 2 ways: using flow cytometry to examine the mononuclear cell and lymphocyte subsets and enzyme-linked immunosorbent assays to examine the cerebrospinal fluid (CSF) levels of cytokines, including monocyte chemoattractant protein-1 (MCP-1), interferon gamma (IFN-gamma) and interleukin-12 (IL-12). The numbers of macrophages in the CSF were increased, in particular during the early part of the acute stage. The levels of MCP-1, which is responsible for the accumulation of macrophages, as well as those of IFN-gamma and IL-12, which play important roles in the activation of macrophages and T helper (Th) 1 cells, respectively, were increased in the CSF of patients compared with the levels found in the controls. Likewise, numbers of activated CD4 + and CD8 + T lymphocytes were increased in the CSF. Since the ratio of CD4/CD8 correlated with the age of the patients, CD8 + T lymphocytes in the CSF might play a more important role in younger children.
Matsubara
T
T
Department of Pediatrics, Yamaguchi University School of Medicine, Ubhe, Japan.
Matsuoka
T
T
Katayama
K
K
Yoshitomi
T
T
Nishikawa
M
M
Ichiyama
T
T
Furukawa
S
S
D002555:Cerebrospinal Fluid / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000134:cerebrospinal fluid*; D004457:Echovirus Infections / Q000134:cerebrospinal fluid / Q000276:immunology* / Q000821:virology; D029822:Enterovirus B, Human / Q000276:immunology*; D005260:Female; D006801:Humans; D007223:Infant; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology*; D008213:Lymphocyte Activation; D016131:Lymphocyte Subsets; D008297:Male; D008587:Meningitis, Viral / Q000134:cerebrospinal fluid / Q000276:immunology* / Q000821:virology
D016207:Cytokines