title: HIP12 is a non-proapoptotic member of a gene family including HIP1, an interacting protein with huntingtin.
abstract: Huntingtin-interacting protein I (HIP1) is a membrane-associated protein that interacts with huntingtin, the protein altered in Huntington disease. HIP1 shows homology to Sla2p, a protein essential for the assembly and function of the cytoskeleton and endocytosis in Saccharomyces cerevisiae. We have determined that the HIP1 gene comprises 32 exons spanning approximately 215 kb of genomic DNA and gives rise to two alternate splice forms termed HIP1-1 and HIP1-2. Additionally, we have identified a novel protein termed HIP12 with significant sequence and biochemical similarities to HIP1 and high sequence similarity to Sla2p. HIP12 differs from HIP1 in its pattern of expression both at the mRNA and protein level. However, HIP1 and HIP12 are both found within the brain and show a similar subcellular distribution pattern. In contrast to HIP1, which is toxic in cell culture, HIP12 does not confer toxicity in the same assay systems. Interestingly, HIP12 does not interact with huntingtin but can interact with HIP1. suggesting a potential interaction in vivo that may influence the function of each respective protein.
authors.affiliation: Department of Medical Genetics, and Centre for Molecular Medicine and Therapeutics, University of British Columbia, Vancouver, Canada.
authors.forename: V S
authors.initials: VS
authors.lastname: Chopra
authors.forename: M
authors.initials: M
authors.lastname: Metzler
authors.forename: D M
authors.initials: DM
authors.lastname: Rasper
authors.forename: A E
authors.initials: AE
authors.lastname: Engqvist-Goldstein
authors.forename: R
authors.initials: R
authors.lastname: Singaraja
authors.forename: L
authors.initials: L
authors.lastname: Gan
authors.forename: K M
authors.initials: KM
authors.lastname: Fichter
authors.forename: K
authors.initials: K
authors.lastname: McCutcheon
authors.forename: D
authors.initials: D
authors.lastname: Drubin
authors.forename: D W
authors.initials: DW
authors.lastname: Nicholson
authors.forename: M R
authors.initials: MR
authors.lastname: Hayden
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D053148:Caspase 3; D020169:Caspases / Q000378:metabolism; D002460:Cell Line; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins; D005656:Fungal Proteins / Q000235:genetics; D015801:Helminth Proteins / Q000235:genetics; D006801:Humans; D000071058:Huntingtin Protein; D007668:Kidney / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*; D009928:Organ Specificity; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D013234:Stem Cells / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C073368:HIP1 protein, human; C086055:HTT protein, human; D015801:Helminth Proteins; D000071058:Huntingtin Protein; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; C082266:SLA2 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C421708:hipr-1 protein, C elegans; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases