[Apoptosis in multiple organs of rats in early stage of polytrauma combined with shock].
OBJECTIVE
To discuss Apoptosis in multiple organs of rats in early stage of polytrauma combined with shock.


METHODS
DNA agarose gel electrophoresis, in situ end labeling (ISEL), light microscope and electron microscope were used and DNA fragmentation percentage (ap%) was detected.


RESULTS
The special ladder pattern for apoptosis was seen in thymus, spleen, liver, lung and intestine, but not in heart, kidney and brain. At 6 hours after resuscitation, the ap% of thymus, spleen, liver, lung and intestine increased together with the severity of trauma. In six-site trauma combined with hemorrhagic shock group, the ap% of these five organs increased significantly at 1 hour after resuscitation and most significantly at 3 hours. At this point, the ap% of spleen, liver, lung and intestine reached peak, and declined gradually afterward. But the ap% of thymus continued to increase after 3 hours and kept stable from 6 hours to 24 hours ISEL showed that there were positive responses of different degrees in these eight organs. It was feand Morphologically most apoptotic cells in the thymus were positioned in the cortex, and those in spleen in the growing center of white pulp, and those in liver in the border area of hepatic lobule and portal area, and apoptosis of multiple kinds of cells including alveolar epithelial cells, vascular endothelial cells and polymorphonuclear neutrophils was induced in lung, and intestinal apoptotic cells laid in the epithelium and lamina propria of mucosa.


CONCLUSIONS
Apoptosis was really induced in thymus, spleen, liver, lung and intestine in early stage of polytrauma combined with shock, which may play a role in early organ injury and late multiple organ failure.
Guan
J
J
Department of Orthopaedics, NanFang Hospital, First Military Medical University, Guangzhou.
Jin
D
D
Jin
L
L
D000818:Animals; D017209:Apoptosis; D005260:Female; D008099:Liver / Q000473:pathology; D008297:Male; D009104:Multiple Trauma / Q000150:complications / Q000473:pathology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012774:Shock, Traumatic / Q000209:etiology / Q000473:pathology*; D013154:Spleen / Q000473:pathology; D013950:Thymus Gland / Q000473:pathology