title: The genetic control of spontaneous and UV-induced mitotic intrachromosomal recombination in the fission yeast Schizosaccharomyces pombe.
abstract: An artificially created non-tandem hetero-allelic duplication was constructed to assay mitotic intrachromosomal recombination in Schizosaccharomyces pombe. Two classes of recombinants could be distinguished: deletion-types, in which one copy of the duplicated sequence and the intervening sequence were lost, and conversion-types which retained the duplication. For spontaneous recombination, compared to wild-type cells, a rad22 mutant (corresponding to a Saccharomyces cerevisiae rad52 mutant) had wild-type levels of deletion-types, but was hypo-recombinant for conversion-types; rad16 (S. cerevisiae rad1), rad22 rad16 (S. cerevisiae rad52 rad1) and swi10 (S. cerevisiae rad10) mutants were hyper-recombinant for both types; rad22 swi10 (S. cerevisiae rad52 rad10) mutants were hypo-recombinant for both types; rhp51 (S. cerevisiae rad51) and rhp54 (S. cerevisiae rad54) mutants were hyper-recombinant for deletion-types, but almost completely lacked conversion-types. For wild-type cells, UV-irradiation induced both types of recombinant, but mainly conversion-types. All of the mutants lacked UV-induced recombination.
authors.affiliation: Department of Biochemistry, University of Oxford, UK.
authors.forename: F
authors.initials: F
authors.lastname: Osman
authors.forename: M
authors.initials: M
authors.lastname: Adriance
authors.forename: S
authors.initials: S
authors.lastname: McCready
mesh_terms: D002470:Cell Survival; D015825:Chromosomes, Fungal / Q000235:genetics*; D004260:DNA Repair; D004271:DNA, Fungal / Q000528:radiation effects; D005785:Gene Conversion / Q000235:genetics*; D017353:Gene Deletion; D006358:Hot Temperature; D008938:Mitosis; D009154:Mutation; D011995:Recombination, Genetic / Q000528:radiation effects*; D012568:Schizosaccharomyces / Q000235:genetics*; D013997:Time Factors
chemical_list: D004271:DNA, Fungal