Murine Bv8 gene maps near a synteny breakpoint of mouse chromosome 6 and human 3p21.
The genomic structure of the murine Bv8 gene was determined in 129/SvJ mouse, and the chromosomal localization was identified. Bv8 has first been characterized from skin secretion of the yellow-bellied toad, Bombina variegata. When injected into rat brain, this polypetide causes hyperalgesia. The murine Bv8 gene was shown to consist of four exons and was localized on chromosome 6 between the microsatellite markers D6Mit66 and D6Mit36 near the gene mem1, whereas the human counterpart was assigned to the non-syntenic region 3p21.1. Furthermore, the primary Bv8 transcript appeared to be alternatively spliced. The first variant contained all four exons yielding a product with a stretch highly enriched in basic amino acids in its central part. This domain is absent in the peptides from frog as well as in a splice variant expressed in mouse testis. A third variant gives rise to a truncated polypeptide.
Jilek
A
A
Institute of Molecular Biology, Department of Biochemistry, Austrian Academy of Sciences, Billrothstr. 11, A-5020, Salzburg, Austria.
Engel
E
E
Beier
D
D
Lepperdinger
G
G
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D029845:Amphibian Proteins; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics*; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D005091:Exons; D006801:Humans; D007438:Introns; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D009479:Neuropeptides; D011506:Proteins / Q000235:genetics*; D021101:Radiation Hybrid Mapping; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D029845:Amphibian Proteins; C121344:Bv8 protein, Bombina variegata; D009479:Neuropeptides; D011506:Proteins; D004247:DNA