title: Differential regulation of amoA and amoB gene copies in Nitrosomonas europaea.
abstract: Nitrosomonas europaea contains two nearly identical copies of the operon, amoCAB, which encodes the ammonia monooxygenase (AMO) enzyme. Cells of N. europaea containing single mutations in either amoA or amoB gene copies were incubated in ammonium both prior to and after exposure to acetylene or light. For each strain, the O(2) consumption rates and amounts of AmoA polypeptide, the active site-containing subunit of AMO, produced in each strain were determined. Strains carrying a mutation in either the amoA(2) or amoB(2) genes responded similarly to wild-type cells, but the strains carrying mutations in the amoA(1) or amoB(1) genes responded differently from the wild-type, or from each other. These results suggest that the copies of amoA and amoB are differentially regulated upon exposure to different external stimuli.
authors.affiliation: Laboratory for Nitrogen Fixation Research, Department of Botany and Plant Pathology, Oregon State University, 2082 Cordley, Corvallis, OR 97331-2902, USA.
authors.forename: L Y
authors.initials: LY
authors.lastname: Stein
authors.forename: L A
authors.initials: LA
authors.lastname: Sayavedra-Soto
authors.forename: N G
authors.initials: NG
authors.lastname: Hommes
authors.forename: D J
authors.initials: DJ
authors.lastname: Arp
mesh_terms: D000114:Acetylene / Q000494:pharmacology; D003470:Culture Media; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects / Q000528:radiation effects; D005798:Genes, Bacterial; D008027:Light; D009606:Nitrosomonas / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D010088:Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D010101:Oxygen Consumption; D010452:Peptide Biosynthesis; D017354:Point Mutation; D000644:Quaternary Ammonium Compounds; D013997:Time Factors
chemical_list: D003470:Culture Media; D000644:Quaternary Ammonium Compounds; D010088:Oxidoreductases; C045582:ammonia monooxygenase; D000114:Acetylene