title: Purification, crystallization and preliminary X-ray crystallographic analysis of human CCG1-interacting factor B.
abstract: A novel human factor CIB (CCG1-interacting factor B) has been isolated using the yeast two-hybrid system. The 22 kDa CIB protein has been expressed in Escherichia coli, purified to homogeneity and crystallized in a form suitable for crystallographic studies. The protein was crystallized in the orthogonal space group P2(1)2(1)2(1), with unit-cell parameters a = 43.60 (2), b = 44.45 (1), c = 110.70 (5) A and one molecule in the asymmetric unit. The crystal diffracted beyond 2.2 A resolution using synchrotron radiation.
authors.affiliation: Horikoshi Gene Selector Project, Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology Corporation (JST), 5-9-6 Tokodai, Tsukuba, Ibaraki 300-2635, Japan.
authors.forename: B
authors.initials: B
authors.lastname: Padmanabhan
authors.forename: T
authors.initials: T
authors.lastname: Kuzuhara
authors.forename: H
authors.initials: H
authors.lastname: Mizuno
authors.forename: M
authors.initials: M
authors.lastname: Horikoshi
mesh_terms: D018797:Cell Cycle Proteins / Q000737:chemistry*; D003461:Crystallography; D018360:Crystallography, X-Ray; D006801:Humans; D016366:Open Reading Frames; D011487:Protein Conformation
chemical_list: D018797:Cell Cycle Proteins