title: Investigation of annexin V binding to lymphocytes after extracorporeal photoimmunotherapy as an early marker of apoptosis.
abstract: BACKGROUND
  Induction of programmed cell death is assumed to be a possible effect of extracorporeal photoimmunotherapy (ECPI).
  
  
  OBJECTIVE
  In the present study lymphocytes of patients with cutaneous T cell lymphoma undergoing ECPI were investigated for early apoptotic events.
  
  
  METHODS
  Annexin V, known for its selective affinity to phospholipids, was used to detect early phases of apoptosis. Simultaneous staining with propidium iodide binding to DNA allowed detection of late apoptotic/necrotic cells.
  
  
  RESULTS
  At 1 h after ECPI, an increase in early apoptotic cells was found indicating a direct effect of ECPI. At 20 h after each ECPI session, a delayed increase in the number of apoptotic lymphocytes was observed in early apoptotic annexin-stained cells and in late apoptotic cells, whereas in nonirradiated cells no remarkable changes were found. Apoptosis was confirmed by altered light scattering properties and DNA fragmentation.
  
  
  CONCLUSIONS
  The apoptotic cell death of reinfused lymphocytes is supposed to be a therapeutic effect of ECPI.
authors.affiliation: Institute of Immunology, Otto von Guericke University, Magdeburg, Germany. anneget.gerber@medizin.uni-magdeburg.de
authors.forename: A
authors.initials: A
authors.lastname: Gerber
authors.forename: M
authors.initials: M
authors.lastname: Bohne
authors.forename: J
authors.initials: J
authors.lastname: Rasch
authors.forename: H
authors.initials: H
authors.lastname: Struy
authors.forename: S
authors.initials: S
authors.lastname: Ansorge
authors.forename: H
authors.initials: H
authors.lastname: Gollnick
mesh_terms: D000368:Aged; D017304:Annexin A5 / Q000378:metabolism*; D017209:Apoptosis; D053938:DNA Fragmentation; D004273:DNA, Neoplasm / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007167:Immunotherapy; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism*; D016410:Lymphoma, T-Cell, Cutaneous / Q000378:metabolism / Q000473:pathology* / Q000628:therapy; D008297:Male; D008875:Middle Aged; D010789:Phototherapy; D011485:Protein Binding; D013997:Time Factors
chemical_list: D017304:Annexin A5; D004273:DNA, Neoplasm