A transgenic rice cell lineage expressing the oat arginine decarboxylase (adc) cDNA constitutively accumulates putrescine in callus and seeds but not in vegetative tissues.
We introduced the oat adc cDNA into rice under the control of the constitutive maize ubiquitin 1 promoter. We studied molecularly and biochemically sixteen independent transgenic plant lines. Significant increases in mRNA levels, ADC enzyme activity and polyamines were measured in transgenic callus. These increases were not maintained in vegetative tissue or seeds in regenerated plants, with the exception of one lineage. This particular lineage showed very significant increases in putrescine preferentially in seeds (up to 10 times compared to wild type and controls transformed with the hpt selectable marker alone). We have demonstrated that in cereals such as rice, over-expression of the oat adc cDNA results in increased accumulation of polyamines at different stages of development. We have also demonstrated that strong constitutive promoters, such as the maize ubiquitin 1 promoter, are sufficient to facilitate heritable high-level polyamine accumulation in seed. Our results demonstrate that by screening adequate numbers of independently derived transgenic plants, it is possible to identify those individuals which express a desired phenotype or genotype.
Noury
M
M
Molecular Biotechnology Unit, John Innes Centre, Norwich Research Park, UK.
Bassie
L
L
Lepri
O
O
Kurek
I
I
Christou
P
P
Capell
T
T
D018554:Avena / Q000201:enzymology* / Q000235:genetics; D015139:Blotting, Southern; D002262:Carboxy-Lyases / Q000235:genetics*; D019070:Cell Lineage; D046508:Culture Techniques; D018076:DNA, Complementary / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D012275:Oryza / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D011073:Polyamines / Q000378:metabolism; D011700:Putrescine / Q000378:metabolism*; D012639:Seeds / Q000235:genetics* / Q000378:metabolism
D018076:DNA, Complementary; D018744:DNA, Plant; D011073:Polyamines; D002262:Carboxy-Lyases; C019722:arginine decarboxylase; D011700:Putrescine