title: Costimulatory molecules expression and cytokine profiles of cord blood mononuclear cells in newborns with low and high risk of developing atopic diseases.
abstract: This study sought to determine predictors of atopic diseases in newborns. We evaluated the levels of expression of costimulatory molecules (CD80 and CD86) and the production of cytokines [interleukin (IL)-12, interferon (IFN)-gamma, IL-4, IL-10] in the cord blood of mononuclear cells in high risk newborns (n = 17), and compared them with those in low risk newborns (n = 25). Fluorescence-activated cell sorter (FACScan) analysis was performed to determine the expressions of CD80 and CD86 on activated B cells and monocytes of both groups. The levels of IL-10, IL-12p40 and IL-12p70 in the supernatant were assayed by enzyme-linked immunosorbent assay (ELISA), and also the mRNA levels by reverse transcription-polymerase chain reaction (RT-PCR). Intracellular staining of IL-4 and IFN-gamma in stimulated mononuclear cells was also performed as well. The expressions of CD80 and CD86 on B cells showed no significant difference between the high and low risk group. There was greater expression of CD86 on the monocytes of low risk newborns as compared to high risk newborns (p < 0.05). When B cells and monocytes isolated from the cord blood of both groups were stimulated with mitogens, the production of IL-10, IL-12p40, and IL-12p70 in the supernatants was not significantly different. The expressions of mRNA of IL-10, IL-12p35, and IL-12p40, and the intracellular staining of IL-4 and IFN-gamma in stimulated mononuclear cells were not significantly different between the two groups. These findings suggested that cytokine profiles in the cord blood cannot predict the development of atopic diseases. Determination of whether preferential expression of costimulatory molecules is of predictive value or not will require further study.
authors.affiliation: Department of Pediatrics, National Taiwan University Hospital, Taipei, ROC.
authors.forename: Y H
authors.initials: YH
authors.lastname: Yang
authors.forename: M C
authors.initials: MC
authors.lastname: Chen
authors.forename: M J
authors.initials: MJ
authors.lastname: Tsai
authors.forename: Y T
authors.initials: YT
authors.lastname: Lin
authors.forename: B L
authors.initials: BL
authors.lastname: Chiang
mesh_terms: D015703:Antigens, CD / Q000097:blood*; D001402:B-Lymphocytes / Q000378:metabolism*; D018122:B7-1 Antigen / Q000097:blood*; D051940:B7-2 Antigen; D016207:Cytokines / Q000096:biosynthesis*; D005312:Fetal Blood / Q000276:immunology*; D006801:Humans; D006967:Hypersensitivity / Q000209:etiology*; D007231:Infant, Newborn; D018664:Interleukin-12 / Q000096:biosynthesis; D008562:Membrane Glycoproteins / Q000097:blood*; D009000:Monocytes / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012306:Risk
chemical_list: D015703:Antigens, CD; D018122:B7-1 Antigen; D051940:B7-2 Antigen; C497964:CD86 protein, human; D016207:Cytokines; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D018664:Interleukin-12