Molecular analysis of tetracycline resistance in Salmonella enterica subsp. enterica serovars Typhimurium, enteritidis, Dublin, Choleraesuis, Hadar and Saintpaul: construction and application of specific gene probes.
A total of 65 epidemiologically unrelated tetracycline-resistant isolates of the six Salmonella enterica subsp. enterica (Salm.) serovars Dublin, Choleraesuis, Typhimurium, Enteritidis, Hadar and Saintpaul were investigated for the presence of tetracycline resistance genes. For this, specific gene probes of the tetracycline resistance genes (tet) of the hybridization classes A, B, C, D, E and G were constructed by cloning PCR-amplified internal segments of the respective tet structural genes. These gene probes were sequenced and used in hybridization experiments with plasmid DNA or endonuclease digested whole cell DNA as targets. Only tet(A) genes were detected on plasmids in all Salm. Dublin isolates as well as in single isolates of Salm. Choleraesuis and Salm. Typhimurium. Genes of the hybridization classes B, C, D and G, but also in some cases those of class A, were located in the chromosomal DNA of the corresponding Salmonella isolates. Restriction fragment length polymorphisms (RFLPs) of tet gene carrying fragments were detected in chromosomally tetracycline-resistant isolates. These RFLPs might represent valuable additional tools for the identification and characterization of tetracycline-resistant Salmonella isolates.
Frech
G
G
Institut für Tierzucht und Tierverhalten der Bundesforschungsanstalt für Landwirtschaft Braunschweig, Celle, Germany.
Schwarz
S
S
D017920:Antiporters / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D012097:Repressor Proteins / Q000235:genetics*; D019779:Salmonella enterica / Q000235:genetics* / Q000302:isolation & purification; D013753:Tetracycline Resistance / Q000235:genetics*; D014157:Transcription Factors
D017920:Antiporters; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D012097:Repressor Proteins; C086977:TETP protein, Clostridium perfringens; C107173:TetC protein, Bacteria; C107174:TetD protein, E coli; D014157:Transcription Factors; C079051:tetA protein, Bacteria