Detection and nucleotide analysis of RNA5 of beet necrotic yellow vein virus isolated in China.
Using the RT-PCR method, five isolates of beet necrotic yellow vein virus from China were used for the RNA5 genomic component detection and sequence analysis. The result showed that RNA5 was only found in the isolates from Baotou and Hohhot, but not in those of Xingjiang, Helongjiang, and Wulate of Inner Mongolia. The RNA5 components had 1338 nucleotides and 1358 nucleotides in the length of Baotou and Hohhot isolates, respectively, in which the single open reading frame (ORF) encoding for a proteins of 26kD were contained. Compared with the published sequences of F72 and D5 isolates, these RNA5 components shared the identity of 93.7% approximately 98.5% in nucleotide acids and 91.8%-98.2% in deduced amino acids.
Dawei
L
L
National Laboratory for Agrobiotechnology, China Agricultural University, Beijing.
Jialin
Y
Y
Chenggui
H
H
Tao
L
L
Shucai
Q
Q
Koenig
R
R
Yi
L
L
D001483:Base Sequence; D002681:China; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D008969:Molecular Sequence Data; D010942:Plant Viruses / Q000235:genetics* / Q000472:pathogenicity; D012328:RNA Viruses / Q000235:genetics* / Q000472:pathogenicity; D012367:RNA, Viral / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D012367:RNA, Viral