Delivery of antisense oligonucleotides to neuroblastoma cells.
pH-Sensitive liposomes composed of dioleoylphosphatidylethanolamine and cholesterol hemisuccinate (3:2 mol/mol) were applied in delivery of antisense oligodeoxynucleotides (asODN) into NG 108-15 neuroblastoma and glioma cells. Fluorescently labelled asODN were entrapped in liposomes by a modified freeze-thawing method (20% encapsulation efficiency). The uptake of asODN (free or entrapped in liposomes) by NG 108-15 cells was monitored by fluorescence-activated cell sorting and confocal microscopy. Delivery of asODN was significantly improved when antisense were entrapped in liposomes as compared to free (nonliposomal) asODN. The uptake was dose-dependent and optimum was achieved after 2 h incubation.
Skalko-Basnet
N
N
Department of Pharmacology, Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, Sugitani, Japan.
Tohda
M
M
Watanabe
H
H
D016503:Drug Delivery Systems; D016650:Fluorescein-5-isothiocyanate; D008081:Liposomes / Q000737:chemistry / Q000493:pharmacokinetics*; D009447:Neuroblastoma; D016376:Oligonucleotides, Antisense / Q000493:pharmacokinetics*; D013696:Temperature; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000187:drug effects*
D008081:Liposomes; D016376:Oligonucleotides, Antisense; D016650:Fluorescein-5-isothiocyanate