Structural analysis of a defective transfer-like region in a staphylococcal aminoglycoside resistance plasmid.
Staphylococcus epidermidis clinical isolate MH6502 contained the 51. 9-kb nonconjugal plasmid pMH6502, which has homology to a major part of the transfer gene region of a known conjugal plasmid. Plasmid pMH6502 mediates aminoglycoside and ethidium bromide resistance. During restriction digest analysis of pMH6502, a double logarithmic regression of marker data gave a better linear relationship than a semi-logarithmic one. The analysis indicated several differences in the transfer-like region of pMH6502 compared to the analogous region of the S. aureus conjugal plasmid pG01. The transfer-like region was in the opposite orientation compared to pG01. An EcoRI site that is within the transfer-like region of pMH6502, has no analogue in pG01. A HindIII site, located outside a 6.3-kb EcoRI fragment in the transfer gene region of pG01, is inside the analogous fragment of pMH6502. A model is proposed to describe how a conjugal ancestral plasmid of pMH6502 could alter to its present form.
von David
W
W
Dept. of Basic Medical Science, School of Medicine, University of Missouri-Kansas City, 2411 Holmes, Kansas City, MO 64108-2792, USA.
Rogolsky
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M
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000737:chemistry; D004352:Drug Resistance, Microbial / Q000235:genetics; D006801:Humans; D009690:Nucleic Acid Conformation; D010957:Plasmids / Q000737:chemistry; D015183:Restriction Mapping / Q000379:methods; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics*; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000235:genetics*
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial