[Study on the effects of salicylic acid on taxol biosynthesis].
The influence of salicylic acid on the production of taxanes in plant cell culture was studied. Experimental results showed that addition of salicylic acid at concentration of 0.1 mg/L could enhance the production of taxol to three-fold. The concentration of 10-DAB and baccatin III was also increasing while taxol concentration increases under salicylic acid elicitation. On the basis of the kinetic analysis about the simplified taxol biosynthesis pathway, a probable reason that salicylic acid improves the rate of 10-DAB producing reaction was introduced. The results above can direct its inducing mechanic research and provide the basis of multiple-elicitors synergism. The concentration of taxol arrives at 39 mg.L-1 induced by salicylic acid with silver nitrate, being 150 percent of the sum of taxol obtained under elicitation of salicylic acid and silver nitrate respectively.
Miao
Z Q
ZQ
Department of Biochemical Engineering, Tianjin.
Wei
Z J
ZJ
Yuan
Y J
YJ
D000972:Antineoplastic Agents, Phytogenic / Q000096:biosynthesis*; D017239:Paclitaxel / Q000096:biosynthesis*; D020156:Salicylic Acid / Q000494:pharmacology*; D012835:Silver Nitrate / Q000494:pharmacology
D000972:Antineoplastic Agents, Phytogenic; D012835:Silver Nitrate; D020156:Salicylic Acid; D017239:Paclitaxel