Effect of genomic and subgenomic leader sequences of potato leafroll virus on gene expression.
The effect of the genomic and subgenomic leader sequence of potato leafroll polerovirus on the efficiency of translation of the downstream located genes has been studied. The results obtained in vitro and in vivo indicate that neither leader sequence functions as translational enhancer, a generally important feature of leader sequences. Deletion analyses demonstrated that both leader sequences not only decrease translation of the downstream located genes but also alter the ratio of the synthesized proteins. A correlation between the in vitro and in vivo results can be established in the case of the subgenomic leader sequence.
Juszczuk
M
M
Instytut Biochemii i Biofizyki, PAN, Ul, Pawińskiego 5A, 02-106, Warsaw, Poland.
Paczkowska
E
E
Sadowy
E
E
Zagórski
W
W
Hulanicka
D M
DM
D001483:Base Sequence; D015967:Gene Expression Regulation, Viral; D017930:Genes, Reporter / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics; D016679:Genome, Viral; D005966:Glucuronidase / Q000032:analysis / Q000235:genetics; D017793:Luteovirus / Q000235:genetics*; D008970:Molecular Weight; D016366:Open Reading Frames / Q000235:genetics; D014176:Protein Biosynthesis; D011523:Protoplasts / Q000821:virology; D020038:RNA, Spliced Leader / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D011198:Solanum tuberosum / Q000821:virology*; D014158:Transcription, Genetic; D014162:Transfection; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics
D020038:RNA, Spliced Leader; D012367:RNA, Viral; D014764:Viral Proteins; D005966:Glucuronidase