Differential RNA cleavage and polyadenylation of the glutamate transporter EAAT2 in the human brain.
We cloned four novel transcripts of the excitatory amino acid transporter 2, named EAAT2/3UT1-4, resulting from differential cleavage and polyadenylation. Tandem poly (A) sites were found to be functional at 72, 654, 973 nucleotides and more than 2 kb downstream of the stop codon. A tissue-specific expression was identified for 3'-variants of the EAAT2 RNA, most prominently for EAAT2/3UT4 (hippocampus>cortex>>cerebellum>thalamus) as demonstrated by Northern blot analysis and quantitative PCR. We conclude, that alternative poly (A) selection may contribute to the reported differential EAAT2 protein expression under normal and diseased conditions.
Münch
C
C
Department of Neurology, University of Ulm, Albert-Einstein-Allee 11, 89081, Ulm, Germany.
Schwalenstöcker
B
B
Hermann
C
C
Cirovic
S
S
Stamm
S
S
Ludolph
A
A
Meyer
T
T
D001483:Base Sequence; D015152:Blotting, Northern; D001923:Brain Chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D017931:DNA Primers; D027342:Excitatory Amino Acid Transporter 2; D015870:Gene Expression / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009410:Nerve Degeneration / Q000235:genetics / Q000503:physiopathology; D012323:RNA Processing, Post-Transcriptional / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D017981:Receptors, Neurotransmitter / Q000235:genetics*; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D027342:Excitatory Amino Acid Transporter 2; D012333:RNA, Messenger; D017981:Receptors, Neurotransmitter