Sera of patients suffering from inflammatory diseases contain group IIA but not group V phospholipase A(2).
During recent years, the high phospholipase A(2) (PLA(2)) concentrations at sites of inflammation and in circulation in several life-threatening diseases, such as sepsis, multi-organ dysfunction and acute respiratory distress syndrome, has generally been ascribed to the non-pancreatic group IIA PLA(2). Recently the family of secreted low molecular mass PLA(2) enzymes has rapidly expanded. In some cases, a newly described enzyme appeared to be cross-reactive with antibodies against the group IIA enzyme. For this reason, reports describing the expression of group IIA PLA(2) during inflammatory conditions need to be reevaluated. Here we describe the identification of the PLA(2) activity in sera of acute chest syndrome patients and in sera of trauma victims. In both cases, the PLA(2) activity was identified as group IIA. This classification was based upon cross-reactivity with monoclonal antibodies against group IIA PLA(2) which do not recognize the recombinant human group V enzyme. Moreover, purification of the enzymatic activity from the two sera followed by N-terminal amino acid sequence analyses revealed only the presence of group IIA enzyme.
Aarsman
A J
AJ
Center for Biomembranes and Lipid Enzymology, Utrecht University, Utrecht, The Netherlands. a.j.aarsman@chem.uu.nl
Neys
F W
FW
van der Helm
H A
HA
Kuypers
F A
FA
van den Bosch
H
H
D000755:Anemia, Sickle Cell / Q000150:complications / Q000201:enzymology; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D016022:Case-Control Studies; D002637:Chest Pain / Q000201:enzymology / Q000209:etiology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007249:Inflammation / Q000201:enzymology*; D010741:Phospholipases A / Q000097:blood* / Q000145:classification* / Q000276:immunology; D013577:Syndrome; D014947:Wounds and Injuries / Q000201:enzymology
D000911:Antibodies, Monoclonal; D010741:Phospholipases A