title: Response of chickens to infection with Newcastle disease virus isolated from a guinea fowl.
abstract: An isolate of Newcastle disease virus obtained from a guinea fowl was characterized as a viscerotropic velogenic strain based upon pathogenicity index studies. Following inoculation of the viral isolate oronasally into 3-week-old chickens, clinical signs appeared after an incubation period of 4-5 days and included dullness, depression, dyspnoea, diarrhoea and leg paralysis. The virus caused a mortality of 56% with haemorrhages at the tip of the glands of the proventriculus and caecal tonsil. Histopathological changes were prominent in the lymphoid organs, being characterized by depletion, degeneration and necrosis of the lymphoid tissues. The brain was the first organ affected, with changes being noticed 3 days after infection. Isolation of virus from various organs was more frequent from 5 to 10 days after infection, but the virus persisted in some of the organs until 21 days after infection. In spite of the high mortality, a good immune response was elicited by the isolate, as was evident from the antibody titre.
authors.affiliation: Division of Health, CSWRI, Rajasthan, India.
authors.forename: S
authors.initials: S
authors.lastname: Mishra
authors.forename: J M
authors.initials: JM
authors.lastname: Kataria
authors.forename: K C
authors.initials: KC
authors.lastname: Verma
authors.forename: R L
authors.initials: RL
authors.lastname: Sah
mesh_terms: D000818:Animals; D000835:Animals, Wild; D000914:Antibodies, Viral / Q000097:blood; D001717:Birds; D001921:Brain / Q000382:microbiology / Q000473:pathology; D002060:Bursa of Fabricius / Q000382:microbiology / Q000473:pathology; D002432:Cecum / Q000382:microbiology / Q000473:pathology; D002642:Chick Embryo; D002645:Chickens / Q000821:virology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006385:Hemagglutination Inhibition Tests / Q000662:veterinary; D007668:Kidney / Q000382:microbiology / Q000473:pathology; D007928:Lethal Dose 50; D008099:Liver / Q000382:microbiology / Q000473:pathology; D008168:Lung / Q000382:microbiology / Q000473:pathology; D009500:Neutralization Tests / Q000662:veterinary; D009521:Newcastle Disease / Q000276:immunology* / Q000635:transmission; D009522:Newcastle disease virus / Q000472:pathogenicity*; D013154:Spleen / Q000382:microbiology / Q000473:pathology; D014132:Trachea / Q000382:microbiology / Q000473:pathology
chemical_list: D000914:Antibodies, Viral