Cell encapsulation with alginate and alpha-phenoxycinnamylidene-acetylated poly(allylamine).
In general, microcapsules prepared from alginate and polycations lack mechanical strength because the interaction between alginate and polycations is ionic instead of covalent, which represents a much stronger bond. To increase the mechanical strength of the capsule, we prepared photosensitive microcapsules that could form covalent bonds between polymers in the capsular membrane by light irradiation. Two types of photosensitive poly(allylamine), with 5% and 10% of amino groups modified by alpha-phenoxycinnamylidene acetylchloride, were synthesized. Both photopolymers exhibited an absorption maximum at 325 nm and were capable of crosslinking upon light exposure. These photosensitive polymers were used for the preparation of microcapsules. The capsules formed from this photosensitive poly(allylamine) and alginate were strengthened significantly by light irradiation. Only 28% of the microcapsules prepared from the 5%-modified photopolymer fractured after 48 h of shaking at 150 rpm. This fracture percentage is much lower when compared with the 60% of capsules fractured when prepared from the untreated poly(allylamine). By using poly(allylamine) at 10% modification, the mechanical strength was improved only slightly, with 26% of capsules fractured. Analysis of the permeability test indicated that the photo-crosslinked capsular membrane was freely permeable to cytochrome c and myoglobin, but less permeable to serum albumin. The encapsulation method was used to entrap and culture IW32 mouse leukemia cells. The cells proliferated to a density of about 1.1 x 10(7) cells/mL in the capsules after 7 days of cultivation. Concurrently, the concentration of erythropoietin in the microcapsules increased to 800 mU/mL. This new encapsulation technique has great potential in the application of a bioindustrial cell-culturing process.
Lu
M Z
MZ
Institute of Biomedical Engineering, National Yang Ming University, Shih-Pai, Taipei, Taiwan, ROC.
Lan
H L
HL
Wang
F F
FF
Chang
S J
SJ
Wang
Y J
YJ
D000464:Alginates / Q000737:chemistry* / Q000528:radiation effects; D000499:Allylamine / Q000031:analogs & derivatives* / Q000737:chemistry*; D000818:Animals; D001709:Biotechnology / Q000379:methods*; D002455:Cell Division; D003574:Cytochrome c Group / Q000737:chemistry / Q000378:metabolism; D004921:Erythropoietin / Q000737:chemistry / Q000378:metabolism; D008027:Light; D008422:Materials Testing; D019563:Mechanics; D008567:Membranes, Artificial; D051379:Mice; D009211:Myoglobin / Q000737:chemistry / Q000378:metabolism; D010539:Permeability; D010777:Photochemistry / Q000379:methods; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000464:Alginates; D003574:Cytochrome c Group; D008567:Membranes, Artificial; D009211:Myoglobin; D011506:Proteins; D012709:Serum Albumin; D004921:Erythropoietin; D000499:Allylamine