Effects of butylated hydroxyanisole and butylated hydroxytoluene on DNA adduct formation and arylamines N-acetyltransferase activity in PC-3 cells (human prostate tumor) in vitro.
The effects of butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) on the N-acetyltransferase (NAT) activity and DNA adduct formation in PC-3 cells (human prostate tumor) was studied. PC-3 cells were placed into tissue culture flasks and grown in an incubator as cytosols and intact cells. The BHA or BHT were added to the cytosols and intact cells. The NAT activity in cytosol and intact PC-3 cells were measured by HPLC assaying exhibited for the amounts of N-acetyl-2-aminofluorene and N-acetyl-p-aminobenzoic acid, 2-aminofluorene and p-aminobenzoic acid. The NAT activity in PC-3 cells and cytosols were inhibited by BHA or BHT in a dose-dependent manner; that is, the higher the concentrations of BHA or BHT the higher inhibition of NAT activity. The NAT values of K(m) and V(max) from PC-3 cells were also decreased by BHA or BHT in both cytosols and intact cells. The data also demonstrated concomitant exposure to BHA or BHT decreased AF-DNA adduct formation which was seen in the PC-3 cells. In addition, the formation of DNA adduct was decreased after BHA or BHT exposure. These findings suggested the usefulness of using human cultured PC-3 cells for assessing arylamine-induced DNA adduct formation. Furthermore, the findings illustrate how effectively BHA or BHT reduce the adduct formation.
Yeh
C C
CC
Department of Urology, China Medical College Hospital, Taichung 400, Taiwan, Republic of China.
Chung
J G
JG
Wu
H C
HC
Li
Y C
YC
Lee
Y M
YM
Hung
C F
CF
D001191:Arylamine N-Acetyltransferase / Q000378:metabolism*; D002083:Butylated Hydroxyanisole / Q000633:toxicity*; D002084:Butylated Hydroxytoluene / Q000633:toxicity*; D003600:Cytosol / Q000187:drug effects / Q000201:enzymology; D004247:DNA / Q000187:drug effects*; D018736:DNA Adducts / Q000187:drug effects*; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D005449:Fluorenes / Q000378:metabolism / Q000633:toxicity; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000201:enzymology* / Q000235:genetics; D014407:Tumor Cells, Cultured / Q000187:drug effects
D018736:DNA Adducts; D005449:Fluorenes; D002084:Butylated Hydroxytoluene; D002083:Butylated Hydroxyanisole; C012177:2-aminofluorene; D004247:DNA; D001191:Arylamine N-Acetyltransferase