title: Clinical application of a polymerase chain reaction assay in the diagnosis of pneumonia caused by Rhodococcus equi in a horse.
abstract: Diagnosis of pneumonia caused by Rhodococcus equi can be made more rapidly by use of a polymerase chain reaction (PCR) assay than by use of conventional bacteriologic culture techniques. Use of a PCR assay aids in the differentiation between virulent and avirulent strains of R equi, and the assay may be used to identify R equi in feces and soil of breeding farms.
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh 27606, USA.
authors.forename: S L
authors.initials: SL
authors.lastname: Vivrette
authors.forename: D C
authors.initials: DC
authors.lastname: Sellon
authors.forename: D S
authors.initials: DS
authors.lastname: Gibbons
mesh_terms: D000193:Actinomycetales Infections / Q000175:diagnosis / Q000188:drug therapy / Q000662:veterinary*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D000904:Antibiotics, Antitubercular / Q000627:therapeutic use; D004917:Erythromycin / Q000627:therapeutic use; D005260:Female; D005839:Gentamicins / Q000627:therapeutic use; D006734:Horse Diseases / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D006736:Horses; D007958:Leukocyte Count / Q000662:veterinary; D010406:Penicillins / Q000627:therapeutic use; D010976:Platelet Count / Q000662:veterinary; D018410:Pneumonia, Bacterial / Q000175:diagnosis / Q000188:drug therapy / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D013902:Radiography, Thoracic / Q000662:veterinary; D016929:Rhodococcus equi / Q000235:genetics / Q000472:pathogenicity*; D012293:Rifampin / Q000627:therapeutic use; D014774:Virulence
chemical_list: D000900:Anti-Bacterial Agents; D000904:Antibiotics, Antitubercular; D005839:Gentamicins; D010406:Penicillins; D004917:Erythromycin; D012293:Rifampin