Yeast Ulp1, an Smt3-specific protease, associates with nucleoporins.
Yeast Smt3 is a ubiquitin-like protein similar to the mammalian SUMO-1. Cdc3, a septin component, is known to be modified by Smt3. The level of this modification was affected by Smt3-specific protease mutation ulp1-ts or overexpression of ULP1. By two-hybrid screening, we isolated 5 UIP (Ulp1 interacting protein) genes. UIP1 was identical to NUP42 encoding a component of the nuclear pore complex (NPC). Gle1, another NPC-associating component, also interacted with Ulp1 in the two-hybrid system and co-immunoprecipitation experiment. Thus Ulp1 associates with nucleoporins and may interact with septin rings in the telophase.
Takahashi
Y
Y
Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Mizoi
J
J
Toh-E
A
A
Kikuchi
Y
Y
D002352:Carrier Proteins / Q000378:metabolism; D018797:Cell Cycle Proteins; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism; D022022:Nuclear Pore / Q000378:metabolism; D028861:Nuclear Pore Complex Proteins; D009687:Nuclear Proteins / Q000378:metabolism*; D018272:Porins / Q000378:metabolism; D051304:Profilins; D016601:RNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; D015003:Yeasts
C119724:AGFG1 protein, human; C086662:CDC3 protein, S cerevisiae; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D005656:Fungal Proteins; C101408:GLE1 protein, S cerevisiae; D028861:Nuclear Pore Complex Proteins; D009687:Nuclear Proteins; D018272:Porins; D051304:Profilins; D016601:RNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; D003546:Cysteine Endopeptidases; C118083:Ulp1 protease