title: A basal transcription factor that activates or represses transcription.
abstract: We have identified an activity that is required for transcription of downstream promoter element (DPE)-containing core promoters in vitro. The purified factor was found to be the Drosophila homolog of the transcriptional repressor known as NC2 or Dr1-Drap1. Purified recombinant dNC2 activates DPE-driven promoters and represses TATA-driven promoters. A mutant version of dNC2 can activate DPE promoters but is unable to repress TATA promoters. Thus, the activation and repression functions are distinct. These studies reveal that NC2 (Dr1-Drap1) is a bifunctional basal transcription factor that differentially regulates gene transcription through DPE or TATA box motifs.
authors.affiliation: Section of Molecular Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla, CA 92093-0347, USA.
authors.forename: P J
authors.initials: PJ
authors.lastname: Willy
authors.forename: R
authors.initials: R
authors.lastname: Kobayashi
authors.forename: J T
authors.initials: JT
authors.lastname: Kadonaga
mesh_terms: D000818:Animals; D004330:Drosophila / Q000235:genetics*; D008970:Molecular Weight; D009154:Mutation; D010750:Phosphoproteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D021122:Protein Subunits; D011994:Recombinant Proteins / Q000378:metabolism; D016385:TATA Box; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D014158:Transcription, Genetic; D015533:Transcriptional Activation
chemical_list: D010750:Phosphoproteins; D021122:Protein Subunits; D011994:Recombinant Proteins; D014157:Transcription Factors; C075995:down-regulator of transcription 1