title: Loss of cation-independent mannose 6-phosphate receptor expression promotes the accumulation of lysobisphosphatidic acid in multilamellar bodies.
abstract: A number of recent studies have highlighted the importance of lipid domains within endocytic organelles in the sorting and movement of integral membrane proteins. In particular, considerable attention has become focussed upon the role of the unusual phospholipid lysobisphosphatidic acid (LBPA). This lipid appears to be directly involved in the trafficking of cholesterol and glycosphingolipids, and accumulates in a number of lysosomal storage disorders. Antibody-mediated disruption of LBPA function also leads to mis-sorting of cation-independent mannose 6-phosphate receptors. We now report that the converse is also true, and that spontaneous loss of cation-independent mannose 6-phosphate receptors from a rat fibroblast cell line led to the formation of aberrant late endocytic structures enriched in LBPA. Accumulation of LBPA was directly dependent upon the loss of the receptors, and could be reversed by expression of bovine cation-independent mannose 6-phosphate receptors in the mutant cell line. Ultrastructural analysis indicated that the abnormal organelles were electron-dense, had a multi-lamellar structure, accumulated endocytosed probes, and were distinct from dense-core lysosomes present within the same cells. The late endocytic structures present at steady state within any particular cell likely reflect the balance of membrane traffic through the endocytic pathway of that cell, and the rate of maturation of individual endocytic organelles. Moreover, there is considerable evidence which suggests that cargo receptors also play a direct mechanistic role in membrane trafficking events. Therefore, loss of such a protein may disturb the overall equilibrium of the pathway, and hence cause the accumulation of aberrant organelles. We propose that this mechanism underlies the phenotype of the mutant cell line, and that the formation of inclusion bodies in many lysosomal storage diseases is also due to an imbalance in membrane trafficking within the endocytic pathway.
authors.affiliation: Wellcome Trust Centre for Molecular Mechanisms in Disease, University of Cambridge, Addenbrookes Hospital, Hills Road, Cambridge, CB2 2XY, UK.
authors.forename: B J
authors.initials: BJ
authors.lastname: Reaves
authors.forename: P E
authors.initials: PE
authors.lastname: Row
authors.forename: N A
authors.initials: NA
authors.lastname: Bright
authors.forename: J P
authors.initials: JP
authors.lastname: Luzio
authors.forename: H W
authors.initials: HW
authors.lastname: Davidson
mesh_terms: D000818:Animals; D001343:Autophagy; D002417:Cattle; D002999:Clone Cells; D004705:Endocytosis; D005347:Fibroblasts / Q000502:physiology* / Q000648:ultrastructure*; D005372:Filipin / Q000378:metabolism; D008246:Lysophospholipids / Q000378:metabolism*; D050178:Monoglycerides; D015388:Organelles / Q000502:physiology*; D051381:Rats; D017527:Receptor, IGF Type 2 / Q000235:genetics / Q000502:physiology*; D011994:Recombinant Proteins / Q000378:metabolism; D014162:Transfection
chemical_list: D008246:Lysophospholipids; D050178:Monoglycerides; D017527:Receptor, IGF Type 2; D011994:Recombinant Proteins; C012786:bis(monoacylglyceryl)phosphate; D005372:Filipin