Immunoreactivity for glutamic acid decarboxylase and N-methyl-D-aspartate receptors of intracellularly labeled respiratory neurons in the cat.
In adult cats, immunofluorescence images of glutamic acid decarboxylase (GAD) and N-methyl-D-aspartate (NMDA) receptors were achieved in the ventral respiratory group (VRG) neurons, which had been individually identified by in vivo intracellular recording and labeling with neurobiotin. Among augmenting inspiratory (aug-I), postinspiratory (post-I), and augmenting expiratory (aug-E) neurons labeled, GAD-immunoreactivity was demonstrated only in those neurons that were not antidromically activated (NAA) by stimulation of the vagus nerve and the C2-C3 spinal cord. Substantial immunoreactivity for NMDA receptors was presented in virtually all types of neurons, but lesser reactivity in aug-E bulbospinal neurons. These results suggest that the aug-I, post-I, and aug-E types of NAA neurons are gamma-aminobutyric acid (GABA)ergic and that NMDA receptors distribute in lesser degree in aug-E bulbospinal neurons than in other types of VRG neurons.
Yamazaki
H
H
Department of Pharmacology, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Japan.
Haji
A
A
Okazaki
M
M
Takeda
R
R
D000818:Animals; D001710:Biotin / Q000031:analogs & derivatives*; D002415:Cats; D002574:Cervical Vertebrae; D004558:Electric Stimulation; D005455:Fluorescent Antibody Technique; D005968:Glutamate Decarboxylase / Q000378:metabolism*; D008526:Medulla Oblongata / Q000502:physiology; D009474:Neurons / Q000378:metabolism*; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D012137:Respiratory System / Q000294:innervation*; D013116:Spinal Cord / Q000502:physiology; D014630:Vagus Nerve / Q000502:physiology
D016194:Receptors, N-Methyl-D-Aspartate; C070614:neurobiotin; D001710:Biotin; D005968:Glutamate Decarboxylase