Linseed oil suppresses the anti-beta-2-glycoprotein-I in experimental antiphospholipid syndrome.
The immunomodulatory potential of a diet enriched with n-3 polyunsaturated fatty acids was analysed in naïve mice with experimental antiphospholipid syndrome (APS) induced by active immunization with H-3, a human anti-beta-2-glycoprotein-I (anti-betaGPI) monoclonal antibody (mAb). Fetal loss and other clinical manifestations of APS were prevented in the group of APS mice upon exposure to the enriched n-3 diet compared to the control group. The titers of anti-betaGPI were significantly lower (in O.D. at 405 nm, 1.387+/-0.232 in comparison to non-treated mice 0.637+/-0. 111, P< 0.05). The reduced titer of anti-betaGPI antibodies in the sera of the treated mice was associated with a reduced number of anti-betaGPI forming cells in cultured splenocytes (84+/-14, antibody-forming cells (AFC)/10(5)cells in comparison to 37+/-4 AFC/10(5)cells (P< 0.02).In addition to the suppression of the humoral response in mice with experimental APS fed with linseed oil, we also observed an inhibitory effect on the cellular response. The T-cell response to anti-betaGPI was lower in comparison to mice immunized with H-3 anti-betaGPI mAB, which were kept on a normal diet. These results indicate that polyunsaturated fatty acids may improve clinical and laboratory parameters of APS. The beneficial effects of diets enriched with n-3 should be further examined as a potential mode of therapy for patients with APS.
Reifen
R
R
School of Nutritional Sciences, Faculty of Agriculture, The Hebrew University of Jerusalem, Jerusalem, Israel.
Amital
H
H
Blank
M
M
Sklan
D
D
Berkovich
Z
Z
Gershwin
E
E
Shoenfeld
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000276:immunology; D016736:Antiphospholipid Syndrome / Q000276:immunology*; D001053:Apolipoproteins / Q000276:immunology*; D002478:Cells, Cultured; D004041:Dietary Fats; D019587:Dietary Supplements; D004195:Disease Models, Animal; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D008043:Linseed Oil / Q000494:pharmacology; D008198:Lymph Nodes; D008562:Membrane Glycoproteins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D053482:beta 2-Glycoprotein I
D000911:Antibodies, Monoclonal; D001053:Apolipoproteins; D004041:Dietary Fats; D005227:Fatty Acids; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D053482:beta 2-Glycoprotein I; D008043:Linseed Oil