title: [Measurement of reticulated platelet using whole blood: methodology and clinical application].
abstract: We established simple and rapid method to measure reticulated platelets using whole blood. After whole blood was fixed with paraformaldehyde, sample was stained with thiazole orange and analyzed using flow cytometer. %RP was significantly higher in patients with idiopathic thrombocytopenic purpura(ITP) (27.7 +/- 14.3%) compared with that of normal subjects(11.9 +/- 3.1%), and elevated in 73% of ITP patients. Furthermore, %RP reflected the status of disease very well in a patient with ITP undergoing therapy. Our method can be effectively applied for differential diagnosis and assessment of the status of thrombocytopenic disorders.
authors.affiliation: Central Laboratory for Clinical Investigation, Osaka University Hospital, Suita.
authors.forename: M
authors.initials: M
authors.lastname: Nishiyama
authors.forename: S
authors.initials: S
authors.lastname: Hayashi
authors.forename: Y
authors.initials: Y
authors.lastname: Futsukaichi
authors.forename: E
authors.initials: E
authors.lastname: Suehisa
authors.forename: Y
authors.initials: Y
authors.lastname: Kurata
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D052160:Benzothiazoles; D001792:Blood Platelets / Q000473:pathology*; D003937:Diagnosis, Differential; D005434:Flow Cytometry; D006801:Humans; D008875:Middle Aged; D010976:Platelet Count / Q000379:methods*; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000097:blood; D011804:Quinolines; D013844:Thiazoles
chemical_list: D052160:Benzothiazoles; D011804:Quinolines; D013844:Thiazoles; C051000:thiazole orange