Determination of vitamin B12 in multivitamin tablets and fermentation medium by high-performance liquid chromatography with fluorescence detection.
A novel method for the determination of vitamin B12 by high-performance liquid chromatography with fluorescence detection is reported. The method was simple and highly sensitive with good precision. Vitamin B12 was analyzed by HPLC on a muBondapak C18 column (300x3.9 mm, 10 microm) with methanol-water (30:70) as mobile phase and fluorescence detection at 305 nm (with excitation at 275 nm). The calibration graph was linear from 1.000 to 100.0 ng ml(-1) for vitamin B12 with a correlation coefficient of 0.998 (n=6). The detection limit was 0.1 ng ml(-1). The method was successfully applied to the determination of vitamin B12 in vitamin B12 tablets, multivitamin tablets and fermentation medium. The recovery was from 94 to 102% and the relative standard deviation was in the range of 1.8 to 4.1%.
Li
H B
HB
Department of Botany, The University of Hong Kong, China.
Chen
F
F
Jiang
Y
Y
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003470:Culture Media / Q000737:chemistry*; D005285:Fermentation; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013607:Tablets / Q000737:chemistry*; D014805:Vitamin B 12 / Q000032:analysis*; D014815:Vitamins / Q000737:chemistry*
D003470:Culture Media; D013607:Tablets; D014815:Vitamins; D014805:Vitamin B 12