title: Evidence for oxidative damage in a murine leukemia virus-induced neurodegeneration.
abstract: Vacuolation in cellular organelles within the central nervous system is a common manifestation of oxidative injury. We found that the spongiform vacuolation observed in PVC-211 murine leukemia virus (PVC-MuLV) neurodegeneration was associated with oxidative damage as detected by immunoreactivity for 3-nitrotyrosine and protein carbonyl groups. This oxidative injury was present in brain before or concomitant with the appearance of activated microglia, vacuolation, and gliosis that characterize PVC-MuLV neuropathology. Treatment of infected F344 rat pups with the antioxidant vitamin E transiently protected and prolonged the latency of PVC-MuLV neurodegeneration. Taken together, these findings implicate oxidative damage and lipid peroxidation in the pathogenesis of PVC-MuLV neurodegeneration. This animal model may be useful for studies of mechanisms and potential therapies for progressive neurodegeneration following a well-defined insult.
authors.affiliation: Research Service, Department of Veterans Affairs Medical Center, Baltimore, Maryland 21201, USA. swilt@umaryland.edu
authors.forename: S G
authors.initials: SG
authors.lastname: Wilt
authors.forename: N V
authors.initials: NV
authors.lastname: Dugger
authors.forename: N D
authors.initials: ND
authors.lastname: Hitt
authors.forename: P M
authors.initials: PM
authors.lastname: Hoffman
mesh_terms: D016475:3T3 Cells; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000473:pathology / Q000821:virology; D020805:Central Nervous System Viral Diseases / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology* / Q000821:virology; D019587:Dietary Supplements; D009052:Leukemia Virus, Murine / Q000472:pathogenicity*; D051379:Mice; D017628:Microglia / Q000378:metabolism / Q000473:pathology; D019636:Neurodegenerative Diseases / Q000178:diet therapy / Q000378:metabolism / Q000473:pathology* / Q000821:virology; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D011916:Rats, Inbred F344; D014617:Vacuoles / Q000473:pathology; D014810:Vitamin E / Q000008:administration & dosage
chemical_list: D015415:Biomarkers; D014810:Vitamin E