Metabolism of an ionic contrast medium and the related agents.
Liquid chromatography-atmospheric pressure chemical ionization mass spectrometry was applied to analyze the iodinated compounds and their glycine conjugates. The negative-ion mass spectra of the iodinated compounds gave [M-H]-, [M-COOH]- and [I]- ions. The positive- and negative-ion mass spectra of the glycine conjugates showed abundant [M+H]+ and [M-H]- ions. Fragmentations of the glycine conjugates obtained in the positive-ion mode were different from those in the negative-ion mode, the former providing more useful structural information for the presence of glycine. Mouse kidney mitochondria were more active in glycine conjugation than liver mitochondria. Mono-substituted benzoic acids were conjugated with glycine in liver and kidney, whereas the acids having three functional groups or more did not undergo glycine conjugation in liver and kidney.
Kasuya
F
F
Faculty of Pharmaceutical Sciences, Kobe-Gakuin University, Japan.
Fukui
M
M
Yanagawa
Y
Y
Kimura
A
A
Fujiwara
H
H
D000818:Animals; D001274:Atmospheric Pressure; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003287:Contrast Media / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice
D003287:Contrast Media