title: Evaluation of six immunoassays for detection of dengue virus-specific immunoglobulin M and G antibodies.
abstract: The performance of six commercially available immunoassay systems for the detection of dengue virus-specific immunoglobulin M (IgM) and IgG antibodies in serum was evaluated. These included two IgM and IgG enzyme immunoassays (EIA) from MRL Laboratories and PanBio, a rapid immunochromatographic test (RIT) from PanBio, immunofluorescence assays (IFA) from Progen, a dot blot assay from Genelabs, and a dipstick EIA from Integrated Diagnostics (INDX). For this study a panel of 132 serum samples, including 90 serum samples from patients with suspected dengue virus infection and 42 serum samples from patients with other viral infections, was used. In addition, serial serum samples from two monkeys experimentally immunized and challenged with dengue virus type 2 were used. Results were considered conclusive when concordant results were obtained with four of the six antibody-specific assays. Based on this definition, the calculated overall agreement for the human serum samples for the respective IgM immunoassays was 97% (128 of 132), with 34% (45 of 132) positive serum samples, 63% (83 of 132) negative samples, and 3% of samples (4 of 132) showing discordant results. The calculated overall agreement for the IgG assays was 94% (124 of 132), with 49% (65 of 132) positive, 45% (59 of 132) negative, and 6% (8 of 132) discordant results, respectively. The sensitivities of the dengue virus-specific assays evaluated varied between 71 and 100% for IgM and between 52 and 100% for IgG, with specificities of 86 to 96% and 81 to 100%, respectively. The relative sensitivities of the respective IgM assays measured with the monkey serum samples were comparable with those obtained with 12 serial serum samples from humans. Overall performance, based on the sum of the agreement, sensitivity, specificity, and Kappa statistics of the IgM and IgG immunoassays, showed that the antibody detection systems from INDX and Genelabs and the MRL and PanBio EIA are useful and reliable assays for dengue virus serodiagnosis.
authors.affiliation: Laboratory for Exotic Viral Infections, WHO Center for Arboviruses and Hemorrhagic Fevers Reference and Research, Institute of Virology, Erasmus Medical Center Rotterdam, 3000 CA Rotterdam, The Netherlands. groen@viro.fgg.eur.nl
authors.forename: J
authors.initials: J
authors.lastname: Groen
authors.forename: P
authors.initials: P
authors.lastname: Koraka
authors.forename: J
authors.initials: J
authors.lastname: Velzing
authors.forename: C
authors.initials: C
authors.lastname: Copra
authors.forename: A D
authors.initials: AD
authors.lastname: Osterhaus
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000918:Antibody Specificity; D003715:Dengue / Q000175:diagnosis / Q000276:immunology; D003716:Dengue Virus / Q000276:immunology*; D005455:Fluorescent Antibody Technique / Q000706:statistics & numerical data; D007114:Immunization; D007118:Immunoassay / Q000379:methods* / Q000706:statistics & numerical data; D015151:Immunoblotting / Q000379:methods / Q000706:statistics & numerical data; D007124:Immunoenzyme Techniques / Q000379:methods / Q000706:statistics & numerical data; D007074:Immunoglobulin G / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood*; D007700:Kinetics; D008252:Macaca fascicularis; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods / Q000706:statistics & numerical data; D014765:Viral Vaccines / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D014765:Viral Vaccines