title: Identification of genes responsive to BPDE treatment in HeLa cells using cDNA expression assays.
abstract: Genotoxic stresses induce cellular responses that can be observed at the level of gene expression. We have studied changes in gene expression following BPDE exposure in HeLa cells by using a cDNA expression array of 597 human genes. After a 53-hr exposure to 0.4 microM BPDE, nine genes were upregulated. The protein products of these genes are: fos-related antigen 2, apoptotic cysteine protease MCH4, DB1 (zinc finger protein 91), transcription factor ETR103, integrin alpha, interleukin-4, interleukin-6, 23-kDa highly basic protein, and ribosomal protein S9. We observed the downregulation of gene expression of three genes: heat-shock protein 27, DNA-binding protein TAX, and NADH-ubiquinone oxidoreductase B18 subunit. These results suggest unknown functions or regulatory circuits for several of the responsive genes and demonstrate the complexity of cellular responses to genotoxic insults.
authors.affiliation: Centre for Environmental Health and the Department of Biology, University of Victoria, Victoria, British Columbia, Canada.
authors.forename: Z
authors.initials: Z
authors.lastname: Yu
authors.forename: B N
authors.initials: BN
authors.lastname: Ford
authors.forename: B W
authors.initials: BW
authors.lastname: Glickman
mesh_terms: D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide / Q000633:toxicity*; D002273:Carcinogens / Q000633:toxicity; D018076:DNA, Complementary / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects*; D006367:HeLa Cells; D006801:Humans; D009152:Mutagenicity Tests; D011506:Proteins / Q000235:genetics; D014162:Transfection
chemical_list: D002273:Carcinogens; D018076:DNA, Complementary; D011506:Proteins; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide