Nickel absorption and distribution from rat small intestine in situ.
The aim of this work was to study the absorption of nickel chloride in rats by means of the intestinal perfusion in situ technique at nickel concentrations of 1, 5, 10, 25, and 100 mg/L. Active transport and facilitated diffusion seem to play an important role in the intestinal absorption of nickel at concentrations < or = 10 mg/L. At higher concentrations, the absorption rate would be limited by saturation of the carriers. The distribution of the absorbed nickel was studied by intestinal perfusion of a 10-mg Ni/L solution for 30 or 60 min. Both in concentration and amount, the jejunum showed the higher values of absorbed nickel, followed by the kidneys and liver. When all of the collected organs (brain, heart, liver, lungs, spleen, kidneys, and testicles) and blood, but not the small intestine, are analyzed following a 60-min perfusion, it was found that 1% of the initial concentration had passed through the intestinal barrier.
Arnich
N
N
Département Environnement et Santé Publique, Faculté de Médecine, Université Henri Poincaré, Vandoeuvre les Nancy, France.
Lanhers
M C
MC
Cunat
L
L
Joyeux
M
M
Burnel
D
D
D000818:Animals; D004396:Coloring Agents; D007408:Intestinal Absorption / Q000502:physiology*; D007421:Intestine, Small / Q000378:metabolism*; D008297:Male; D009532:Nickel / Q000097:blood / Q000493:pharmacokinetics*; D010477:Perfusion; D010637:Phenolsulfonphthalein; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution
D004396:Coloring Agents; D009532:Nickel; D010637:Phenolsulfonphthalein