title: Molecular structure and evolution of DNA sequences located at the alpha satellite boundary of chromosome 20.
abstract: We have isolated and characterised one PAC clone (dJ233C1) containing a linkage between alphoid and non-alphoid DNA. The non-alphoid DNA was found to map at the pericentromeric region of chromosome 20, both on p and q sides, and to contain homologies with one contig (ctg176, Sanger Centre), also located in the same chromosome region. At variance with the chromosome specificity shown by the majority of non-alphoid DNA, a subset of alphoid repeats derived from the PAC yielded FISH hybridisation signals located at the centromeric region of several human chromosomes, belonging to three different suprachromosomal families. The evolutionary conservation of this boundary region was investigated by comparative FISH experiments on chromosomes from great apes. The non-alphoid DNA was found to have undergone events of expansion and transposition to different pericentromeric regions of great apes chromosomes. Alphoid sequences revealed a very wide distribution of FISH signals in the great apes. The pattern was substantially discordant with the data available in the literature, which is essentially derived from the central alphoid subset. These results add further support to the emerging opinion that the pericentromeric regions are high plastics, and that the alpha satellite junctions do not share the evolutionary history with the main subsets.
authors.affiliation: Dipartimento di Biologia e Genetica per le Scienze Mediche, Università di Milano, 20133, Milan, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Bassi
authors.forename: I
authors.initials: I
authors.lastname: Magnani
authors.forename: N
authors.initials: N
authors.lastname: Sacchi
authors.forename: S
authors.initials: S
authors.lastname: Saccone
authors.forename: A
authors.initials: A
authors.lastname: Ventura
authors.forename: M
authors.initials: M
authors.lastname: Rocchi
authors.forename: A
authors.initials: A
authors.lastname: Marozzi
authors.forename: E
authors.initials: E
authors.lastname: Ginelli
authors.forename: R
authors.initials: R
authors.lastname: Meneveri
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002890:Chromosomes, Human, Pair 20 / Q000235:genetics*; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D004276:DNA, Satellite / Q000235:genetics*; D019143:Evolution, Molecular; D005260:Female; D015186:Hominidae; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
chemical_list: D004276:DNA, Satellite; D004247:DNA