Genotoxic and mono-oxygenase system effects of the fungicide maneb.
The in vivo effects of a commercial preparation of maneb on mono-oxygenase activities of hepatic microsomes of basal and induced rats were examined. In vitro experiments with the D7 strain of yeast Saccharomyces cerevisiae were also performed. In both basal and induced rats maneb caused a decrease in cytochrome P-450 content and aniline hydroxylase. Immunoblotting analysis using anti-P-450 IIE1 antibodies confirmed the data obtained for aniline hydroxylase activity. Maneb was toxic in cells of S. cerevisiae. On the basis of in vivo and in vitro experiments it can be concluded that maneb possesses a toxic activity attributable to its main metabolite ethylene thiourea. Immunoblotting analysis indicates that maneb biotransformation influences the IIEI P-450 isoform.
Bertini
S
S
Istituto di Ispezione degli Alimenti di Origine Animale, Parma, Italy.
Del Carratore
R
R
Giorgi
M
M
Bronzetti
G
G
Della Croce
C
C
D000815:Aniline Hydroxylase / Q000096:biosynthesis; D000818:Animals; D001711:Biotransformation; D015153:Blotting, Western; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D004790:Enzyme Induction; D005659:Fungicides, Industrial / Q000378:metabolism / Q000633:toxicity*; D005785:Gene Conversion / Q000187:drug effects; D008344:Maneb / Q000378:metabolism / Q000633:toxicity*; D008862:Microsomes, Liver / Q000187:drug effects* / Q000201:enzymology; D009152:Mutagenicity Tests; D009153:Mutagens / Q000378:metabolism / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development
D005659:Fungicides, Industrial; D009153:Mutagens; D008344:Maneb; D003577:Cytochrome P-450 Enzyme System; D000815:Aniline Hydroxylase