title: Attachment and penetration of canine herpesvirus 1 in non-permissive cells.
abstract: Canine herpesvirus 1 (CHV-1) has a relatively narrow host cell range when compared to other alphaherpesviruses. The early events of CHV-1 infection in a permissive Madin-Darby canine kidney (MDCK) and non-permissive cell lines. In order to quantify attachment and penetration, were investigated quantitative competitive PCR (QCPCR) method was established for quantitation of CHV-1 DNA. In all non-permissive cells tested, no significant decrease in viral attachment was observed. When CHV-1 was treated with heparin, viral attachment to MDCK cells was reduced by 25% of the input CHV-1 attached to MDCK cells even in the presence of 50 microg/ml heparin. However, the attachment of CHV-1 to non-permissive cells was severely impaired by heparin treatment. In permissive MDCK cells, about 80% of attached CHV-1 penetrated into cells. However, only 4-10% of CHV-1 attached to non-permissive cells penetrated into cells. Our data indicated that CHV-1, like other herpesviruses, attached to permissive MDCK cells through two mechanisms: the first one is through the interaction mediated by heparan sulfate (HS) on the cell surface and the second involves unidentified viral component and the cellular receptor. In contrast, the non-permissive cells lacked the cellular receptor for the second attachment mechanism and the defect in viral penetration into non-permissive cell might be related to the lack of the cellular receptor.
authors.affiliation: Department of Global Animal Resource Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nakamichi
authors.forename: K
authors.initials: K
authors.lastname: Ohara
authors.forename: Y
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: H
authors.initials: H
authors.lastname: Otsuka
mesh_terms: D000818:Animals; D000906:Antibodies; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods / Q000662:veterinary; D002460:Cell Line; D004285:Dogs; D006493:Heparin / Q000494:pharmacology; D002184:Herpesvirus 1, Canid / Q000187:drug effects / Q000472:pathogenicity*; D009500:Neutralization Tests / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011817:Rabbits; D013552:Swine; D014759:Viral Envelope Proteins / Q000276:immunology
chemical_list: D000906:Antibodies; D014759:Viral Envelope Proteins; C088592:glycoprotein B, Canid herpesvirus 1; C088593:glycoprotein C, Canid herpesvirus 1; C088594:glycoprotein D, Canid herpesvirus 1; D006493:Heparin