Novel procedure for identification of compounds inhibitory to transcription of genes involved in mycotoxin biosynthesis.
A novel assay is described for the identification and isolation of compounds that inhibit the transcription of genes involved in mycotoxin biosynthesis. The thin-layer chromatography-based assay was used to screen plant extracts for compounds that would inhibit the expression of the beta-glucuronidase reporter gene under the control of an aflatoxin biosynthesis gene promoter in Aspergillus parasiticus. The assay was used to track purification of an inhibitory compound, cp2, from extracts of black pepper (Piper nigrum). Cp2 did not inhibit mycelial growth or the expression of the beta-tubulin gene but did inhibit aflatoxin biosynthesis at the transcriptional level. Applications of cp2 to the control of mycotoxins are discussed.
Annis
S L
SL
Department of Botany and Plant Pathology and Department of Food Science and Human Nutrition, Michigan State University, East Lansing, Michigan 48824, USA.
Velasquez
L
L
Xu
H
H
Hammerschmidt
R
R
Linz
J
J
Trail
F
F
D000348:Aflatoxins / Q000096:biosynthesis / Q000235:genetics; D001230:Aspergillus / Q000235:genetics* / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D009183:Mycotoxins / Q000096:biosynthesis* / Q000235:genetics*; D010936:Plant Extracts / Q000494:pharmacology; D017365:Spices / Q000032:analysis
D000348:Aflatoxins; D009183:Mycotoxins; D010936:Plant Extracts