Hepatotropism of nonenveloped DNA virus in rhesus monkey infected by transfusion-transmitted virus.
OBJECTIVE
To study wether the nonenveloped DNA virus transmitted via blood transfusion is hepatotropic.


METHODS
Total DNA was extracted from tissues of 5 experimentally infected Rhesus monkeys. A dot hybridization was done with virus double DNA strand probe or single antisense strand probe.


RESULTS
Both single- and double-strand probes were hybridized with DNA of the liver, spleen, stomach, small intestine and colon. The virus was conformed present in most of all the organs when double-strand probe was used. The positive was noted only in the liver and small intestine when single-strand antisense probe was used, which showed that in liver and small intestine might have replicative intermediates of the virus.


CONCLUSIONS
It suggests that nonenveloped DNA virus replicate in the liver and small intestine, so it might be hepatotropic.
Xiao
H
H
Department of Infectious diseases Nanfang Hospital, Guangzhou 510515, China.
Qi
X
X
Yang
S
S
Luo
K
K
D000818:Animals; D004261:DNA Replication; D008099:Liver / Q000821:virology*; D008253:Macaca mulatta; D022783:Torque teno virus / Q000502:physiology*; D014779:Virus Replication