[Effect of 6-dimethylaminopurine on the resumption of meiosis and parthenogenetic activation in mouse oocytes].
Chromatin condensation and germinal vesicle breakdown (GVBD) in mouse cumulus free oocytes was blocked by 2 mmol/L of 6-DMAP in vitro. Mouse oocytes (M II) from superovulated female mice could be activated by 6-DMAP. The activation rates of oocytes (18-19 h post hCG) cultured in CZB medium with 2 mmol/L 6-DMAP for 0.5, 1, 2, 3 h, were 26.1%, 75.2%, 75.8%, 77.3% respectively (Table 1), and the cleavage rates of these activated oocytes were 88.2%, 73.2%, 67.0%, 58.4% respectively (Table 2). The parthenogenetic activation type induced by 6-DMAP was different from that produced by ethanol (Fig.1).
Liu
H L
HL
Jiang Su Academy of Agricultural Sciences, Nanjing.
Fan
B Q
BQ
Wang
H H
HH
Cheng
Y F
YF
Wang
G J
GJ
D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D005260:Female; D008540:Meiosis / Q000187:drug effects; D051379:Mice; D009865:Oocytes / Q000166:cytology*; D010312:Parthenogenesis / Q000187:drug effects*; D047428:Protein Kinase Inhibitors
D047428:Protein Kinase Inhibitors; C001020:N(6),N(6)-dimethyladenine; D000225:Adenine