title: [Tissue culture induced translocation conferring powdery mildew resistance between wheat and Dasypyrum villosum and its marker-assisted selection].
abstract: Glutamate oxaloacetate transaminase (GOT) electrophoretic analyses were performed in 175 regenerants arising from immature embryos of crosses between wheat (Triticum aestivum L.) and 6D/6V substitution stocks. The GOT-V2 coding specific enzyme band was absent in two regenerants, designated 98R149 and 98R159 respectively, originated from cross of Yi 4095 and 6D/6V substitution stock c.v. RW15. Pm21 gene linked SCARs (Sequence Characterized Amplified Regions) analysis indicated that 6VS chromosome arms existed in 98R149 and 98R159. Fluorescence in situ hybridization with total genomic DNA extracted from Dasypyrum villosum (L.) as a probe confirmed the occurrence of translocation between 6V chromosome and a wheat one in the two regenerants mentioned above. 98R149 and 98R159 were immune to powdery mildew (Erysiphe graminisDC. f. sp. tritici) inoculation with mix races collected from Hebei Province. The results of the present paper added another feasible example of useful translocations via tissue culture.
authors.affiliation: State Key Laboratory of Plant Cell and Chromosome Engineering, Chinese Academy of Sciences, Beijing, China.
authors.forename: H J
authors.initials: HJ
authors.lastname: Li
authors.forename: Y W
authors.initials: YW
authors.lastname: Li
authors.forename: Y M
authors.initials: YM
authors.lastname: Zhang
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.forename: B H
authors.initials: BH
authors.lastname: Guo
authors.forename: Z N
authors.initials: ZN
authors.lastname: Wang
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Wen
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Liu
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Zhu
authors.forename: X
authors.initials: X
authors.lastname: Jia
mesh_terms: D001219:Aspartate Aminotransferases / Q000378:metabolism; D046508:Culture Techniques; D005819:Genetic Markers; D010935:Plant Diseases / Q000235:genetics*; D012641:Selection, Genetic; D014178:Translocation, Genetic; D014908:Triticum / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D001219:Aspartate Aminotransferases