title: [Construction of a transformation-competent artificial chromosome (TAC) library of a wheat-Haynaldia villosa translocation line].
abstract: Transformation-competent artificial chromosome (TAC) vector is able to clone and transfer large DNA fragments in plants and is a powerful tool for plant gene isolation and transformation. To clone important genes from wheat, a TAC genomic library was constructed from nuclear DNA of a 6VS/6AL wheat-Haynaldia villosa translocation line that harbor the gene Pm21 for resistance to powdery mildew. The library consists of 2.1 x 10(6) clones with an average DNA insert size of 35 kb, and represents in total 4.9 genome equivalents. The library was stored as clone pools in 96-well plates, and each pool contained about 1000 clones. TAC clones containing gene(s) of interest can be screened by a pooled-PCR/colony-hybridization strategy.
authors.affiliation: Key Laboratory of Crop Cytogenetics, Minstry of Agricultural, Nanjing Agricultural University.
authors.forename: Y D
authors.initials: YD
authors.lastname: Fang
authors.forename: Y G
authors.initials: YG
authors.lastname: Liu
authors.forename: H
authors.initials: H
authors.lastname: Wu
authors.forename: Q Y
authors.initials: QY
authors.lastname: Zhang
authors.forename: P D
authors.initials: PD
authors.lastname: Chen
authors.forename: D J
authors.initials: DJ
authors.lastname: Liu
mesh_terms: D022201:Chromosomes, Artificial; D015723:Gene Library; D014170:Transformation, Genetic; D014178:Translocation, Genetic; D014908:Triticum / Q000235:genetics*