High-throughput protein crystallization.
The combinatorial chemistry industry has made major advances in the handling and mixing of small volumes, and in the development of robust liquid-handling systems. In addition, developments have been made in the area of material handling for the high-throughput drug screening and combinatorial chemistry fields. Lastly, improvements in beamline optics at synchrotron sources have enabled the use of flash-frozen micron-sized (10-50 microm) crystals. The combination of these and other recent advances will make high-throughput protein crystallography possible. Further advances in high-throughput methods of protein crystallography will require application of the above developments and the accumulation of success/failure data in a more systematic manner. Major changes in crystallography technology will emerge based on the data collected by first-generation high-throughput systems.
Stevens
R C
RC
The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA. stevens@scripps.edu
D001331:Automation; D003460:Crystallization; D011506:Proteins / Q000737:chemistry*
D011506:Proteins