title: Purification of proteins using polyhistidine affinity tags.
authors.affiliation: Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309-0215, USA.
authors.forename: J A
authors.initials: JA
authors.lastname: Bornhorst
authors.forename: J J
authors.initials: JJ
authors.lastname: Falke
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006639:Histidine; D007202:Indicators and Reagents; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D009532:Nickel; D009571:Nitrilotriacetic Acid / Q000031:analogs & derivatives; D009942:Organometallic Compounds; D010455:Peptides; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification*
chemical_list: D007202:Indicators and Reagents; D008565:Membrane Proteins; D009942:Organometallic Compounds; D010455:Peptides; D011994:Recombinant Proteins; C033223:polyhistidine; C088321:nickel nitrilotriacetic acid; D006639:Histidine; D009532:Nickel; D009571:Nitrilotriacetic Acid