Tendency modeling: a new approach to obtain simplified kinetic models of metabolism applied to Saccharomyces cerevisiae.
A novel approach to construct kinetic models of metabolic pathways, to be used in metabolic engineering, is presented: the tendency modeling approach. This approach greatly facilitates the construction of these models and can easily be applied to complex metabolic networks. The resulting models contain a minimal number of parameters; identification of their values is straightforward. Use of in vitro obtained information in the identification of the kinetic equations is minimized. The tendency modeling approach has been used to derive a dynamic model of primary metabolism for aerobic growth of Saccharomyces cerevisiae on glucose, in which compartmentation is included. Simulation results obtained with the derived model are satisfying for most of the carbon metabolites that have been measured. Compared to a more detailed model, the simulations of our model are less accurate, but taking into account the much smaller number of kinetic parameters (35 instead of 84), the tendency the modeling approach is considered promising.
Visser
D
D
Kluyver Laboratory for Biotechnology, Delft University of Technology, Delft, The Netherlands. D.Visser@stm.tudelft.nl
van der Heijden
R
R
Mauch
K
K
Reuss
M
M
Heijnen
S
S
D001698:Biomedical Engineering; D003198:Computer Simulation; D007700:Kinetics; D008954:Models, Biological; D015233:Models, Statistical; D012441:Saccharomyces cerevisiae / Q000378:metabolism*