title: Determination of biotin and folate in infant formula and milk by optical biosensor-based immunoassay.
abstract: Biomolecular interaction analysis was evaluated for the automated analysis of biotin- and folate-supplemented infant formulas and milk powders. The technique was configured as a biosensor-based, nonlabeled inhibition immunoassay using monoclonal antibodies raised against analyte-conjugate. Sample extraction conditions were optimized and antibodies were evaluated for cross-reactivity. Performance parameters included a quantitation range of 2-70 ng/mL, recoveries of 86-102%, agreement against assigned reference values for National Institute of Standards and Technology Standard Reference Material 1846, between-laboratory reproducibility relative standard deviation of 9.1% for biotin and 8.1% for folate, respectively, and equivalence against reference microbiological assay methods for both analytes.
authors.affiliation: Anchor Products, Waitoa, New Zealand.
authors.forename: H E
authors.initials: HE
authors.lastname: Indyk
authors.forename: E A
authors.initials: EA
authors.lastname: Evans
authors.forename: M C
authors.initials: MC
authors.lastname: Bostrom Caselunghe
authors.forename: B S
authors.initials: BS
authors.lastname: Persson
authors.forename: P M
authors.initials: PM
authors.lastname: Finglas
authors.forename: D C
authors.initials: DC
authors.lastname: Woollard
authors.forename: E L
authors.initials: EL
authors.lastname: Filonzi
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D015374:Biosensing Techniques; D001710:Biotin / Q000032:analysis*; D002138:Calibration; D005492:Folic Acid / Q000032:analysis*; D005516:Food Microbiology; D006801:Humans; D007118:Immunoassay; D007202:Indicators and Reagents; D007225:Infant Food / Q000032:analysis*; D007231:Infant, Newborn; D008892:Milk / Q000737:chemistry*
chemical_list: D007202:Indicators and Reagents; D001710:Biotin; D005492:Folic Acid