title: Regulation of oxidative phosphorylation in the inner membrane of rat liver mitochondria by calcium ions.
abstract: The effect of accumulation of Ca2+ at physiological concentrations (10(-8)-10(-6) M) on the rates of ATP synthesis and hydrolysis in rat liver mitochondria was studied. An addition of 5 x 10(-7) M Ca2+ resulted in the maximal rates of synthesis and hydrolysis of ATP. Decrease in the concentration of Ca2+ to 10-8 M or its increase to 5 x 10(-6) M inhibited oxidative phosphorylation and ATP hydrolysis. It was found that the rate of oxidative phosphorylation correlated with the phosphorylation level of a 3.5-kD peptide in the mitochondrial inner membrane on varying the Ca2+ concentration. The possible regulation of oxidative phosphorylation in mitochondria by Ca2+ is discussed.
authors.affiliation: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142292, Russia.
authors.forename: Y V
authors.initials: YV
authors.lastname: Evtodienko
authors.forename: T S
authors.initials: TS
authors.lastname: Azarashvili
authors.forename: V V
authors.initials: VV
authors.lastname: Teplova
authors.forename: I V
authors.initials: IV
authors.lastname: Odinokova
authors.forename: N
authors.initials: N
authors.lastname: Saris
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006868:Hydrolysis; D007425:Intracellular Membranes / Q000378:metabolism*; D007477:Ions; D007700:Kinetics; D008930:Mitochondria, Liver / Q000378:metabolism*; D010085:Oxidative Phosphorylation; D010455:Peptides / Q000378:metabolism; D006180:Proton-Translocating ATPases / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D007477:Ions; D010455:Peptides; D000255:Adenosine Triphosphate; D006180:Proton-Translocating ATPases; D002118:Calcium