Detection of extended-spectrum beta-lactamases in klebsiellae with the Oxoid combination disk method.
The Oxoid combination disk method for detecting extended-spectrum beta-lactamases (ESBLs) depends on comparing the inhibition zones of cefpodoxime (10-microg) and cefpodoxime-plus-clavulanate (10- plus 1-microg) disks. The presence of clavulanate enlarged the zones for all of 180 ESBL-producing klebsiellae by >/=5 mm, whereas zones for cefpodoxime-susceptible isolates and cefpodoxime-resistant isolates with AmpC and K1 beta-lactamases were enlarged by </=1 mm. Good discrimination was achieved with either the NCCLS or the British disk method.
Carter
M W
MW
Antibiotic Resistance Monitoring and Reference Laboratory, Central Public Health Laboratory, London NW9 5HT, United Kingdom.
Oakton
K J
KJ
Warner
M
M
Livermore
D M
DM
D001426:Bacterial Proteins; D002442:Ceftazidime / Q000494:pharmacology; D015296:Ceftizoxime / Q000031:analogs & derivatives* / Q000494:pharmacology*; D002511:Cephalosporins / Q000494:pharmacology*; D019818:Clavulanic Acid / Q000494:pharmacology; D006801:Humans; D007709:Klebsiella / Q000187:drug effects / Q000201:enzymology*; D007710:Klebsiella Infections / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology*; D008826:Microbial Sensitivity Tests / Q000379:methods; D001618:beta-Lactamases / Q000378:metabolism*; D000097623:Cefpodoxime
D001426:Bacterial Proteins; D002442:Ceftazidime; D015296:Ceftizoxime; D002511:Cephalosporins; D019818:Clavulanic Acid; D001618:beta-Lactamases; D000097623:Cefpodoxime; C048795:AmpC beta-lactamases