[The effects of FSH,LH and insulin on steroids production by granulosa cells from polycystic ovaries syndrome].
OBJECTIVE
To investigate steroids production of granulosa cells obtained from normal ovaries and polycystic ovaries from polycystic ovary syndrome (PCOS) patients under FSH, LH and insulin stimulation.


METHODS
Granulosa cells of 33 folicles from 18 pairs of normal ovaries and about 100 folicles from 8 pairs of PCOS were cultured. Estrodial (E2) and progesterone (P) accumulation in the medium was determined under different incubated conditions by RIA.


RESULTS
Granulosa cells from PCOS produced more E2(P < 0.05-0.01) under basal, FSH and insulin stimulation and more P (P < 0.05-0.01) under FSH, LH and insulin stimulation than controls. Insulin markedly augmented FSH and LH-stimulated steroid accumulation by two groups of granulosa cells. Granulosa cells from PCOS with peripheral insulin resistance also responded to insulin.


CONCLUSIONS
Enzymes for steroids synthesis in granulosa cells from PCOS may be activated in vivo, and hyperinsulinisma and elevated LH levels in PCOS may be contributable.
Wang
A
A
Department of Gynecology & Obstetrics, Third Hospital, Beijing Medical University.
Lu
C
C
Qiao
J
J
D000328:Adult; D002478:Cells, Cultured; D004958:Estradiol / Q000096:biosynthesis*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D006107:Granulosa Cells / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D007986:Luteinizing Hormone / Q000494:pharmacology; D011085:Polycystic Ovary Syndrome / Q000378:metabolism* / Q000473:pathology; D011374:Progesterone / Q000096:biosynthesis
D007328:Insulin; D011374:Progesterone; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone