[Study of adhesive proteins from neural tissues of the 8-day old chicken embryonic eye].
Two groups of proteins were isolated from the retina and pigment epithelium of eight-day-old chick embryos. Experiments with suspension cultures of retinal cells demonstrated that only the retinal extracts and the fraction of its acidic proteins can stimulate cell aggregation in vitro. Analysis by the method of high-performance liquid chromatography showed that fractions of acidic and basic retinal proteins, which markedly differ in their electric charge and biological activity, have similar composition. To study the effect of these proteins on the morphological and functional state of pigment epithelium in vitro, a new experimental model is proposed, with the posterior segment of the newt (Pleurodeles waltl) eye used as a test tissue. The fraction of basic proteins isolated from the chick embryonic pigment epithelium stabilized cell differentiation in the newt pigment epithelium. The analyzed proteins proved to be biologically active at extremely low doses, corresponding to 10(-12) M solutions.
Krasnov
M S
MS
Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia.
Grigorian
E N
EN
Iamskova
V P
VP
Danilenko
A N
AN
Iamskov
I A
IA
D000818:Animals; D015815:Cell Adhesion Molecules / Q000302:isolation & purification / Q000502:physiology*; D002642:Chick Embryo; D002851:Chromatography, High Pressure Liquid; D023421:Models, Animal; D010857:Pigment Epithelium of Eye / Q000294:innervation / Q000378:metabolism*; D012160:Retina / Q000378:metabolism*; D012455:Salamandridae
D015815:Cell Adhesion Molecules