Suppression of experimental autoimmune myasthenia gravis in IL-10 gene-disrupted mice is associated with reduced B cells and serum cytotoxicity on mouse cell line expressing AChR.
To analyze the role of interleukin-10 (IL-10) in experimental autoimmune myasthenia gravis (EAMG) pathogenesis, we induced clinical EAMG in C57BL/6 and IL-10 gene-knockout (KO) mice. IL-10 KO mice had a lower incidence and severity of EAMG, with less muscle acetylcholine receptor (AChR) loss. AChR-immunized IL-10 KO mice showed a significantly higher AChR-specific proliferative response, altered cytokine response, lower number of class II-positive cells and B-cells, but a greater CD5(+)CD19(+) population than C57BL/6 mice. The lower clinical incidence in IL-10 KO could be explained not by a reduction of the quantity, but by a possible difference in the pathogenicity of anti-AChR antibodies.
Poussin
M A
MA
Department of Microbiology and Immunology, University of Texas Medical Branch, 77555-1070, Galveston, TX, USA.
Goluszko
E
E
Hughes
T K
TK
Duchicella
S I
SI
Christadoss
P
P
D000276:Adjuvants, Immunologic / Q000235:genetics; D000818:Animals; D018941:Antigens, CD19 / Q000032:analysis; D001323:Autoantibodies / Q000097:blood; D001402:B-Lymphocytes / Q000737:chemistry / Q000166:cytology / Q000276:immunology*; D001798:Blood Proteins / Q000276:immunology; D018956:CD5 Antigens / Q000032:analysis; D002455:Cell Division / Q000276:immunology; D002460:Cell Line; D003603:Cytotoxins / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis / Q000276:immunology; D007114:Immunization; D016056:Immunodominant Epitopes / Q000276:immunology; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000096:biosynthesis / Q000276:immunology; D016753:Interleukin-10 / Q000235:genetics* / Q000276:immunology*; D015850:Interleukin-6 / Q000096:biosynthesis / Q000276:immunology; D018655:Lymphocyte Count; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018482:Muscle, Skeletal / Q000737:chemistry / Q000276:immunology; D020720:Myasthenia Gravis, Autoimmune, Experimental / Q000235:genetics* / Q000276:immunology*; D011950:Receptors, Cholinergic / Q000235:genetics / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000276:immunology
D000276:Adjuvants, Immunologic; D018941:Antigens, CD19; D001323:Autoantibodies; D001798:Blood Proteins; D018956:CD5 Antigens; D003603:Cytotoxins; D000939:Epitopes; D000949:Histocompatibility Antigens Class II; D016056:Immunodominant Epitopes; D015850:Interleukin-6; D011950:Receptors, Cholinergic; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007371:Interferon-gamma