[Genetic characterization of subcbnI genes of Chlamydomonas reinhardtii].
Fourteen different revertants of Chlamydomonas reinhardtii recovered with ability of biosynthesis chlorophyll b were hybridized with wile-type strain, and tetrad analysis with random sampling was performed. It appeared that sub genes resulting in cbnI gene to reverse mutation, and localize on the first chromosome. According to its linkage that differences, 5 strains carrying various mutant alleles of suppressor genes were determined. Forward hybridological analysis demonstrated that the sub genes were absent of allelic specificity and had a single genic character in response to suppression. Phenotypic analysis of the sub/Sub diplontic hybrid have verified the dominant character of mutant sub genes. The phenomenon of present various allelic sub genes and all its characters revealed that the possibility of several ways or various regulatory means exists in biosynthesis of chlorophyll b.
Mijit
G
G
Department of Biology, Xinjiang University, Urumqi, China.
Rahman
E
E
Nikulina
K V
KV
Boladhan
Z
Z
Chunaev
A S
AS
Rüdiger
W
W
D000818:Animals; D002338:Carotenoids / Q000172:deficiency*; D016825:Chlamydomonas reinhardtii / Q000235:genetics*; D002734:Chlorophyll / Q000096:biosynthesis* / Q000172:deficiency*; D016153:Genes, Suppressor; D009154:Mutation; D024341:Xanthophylls
D024341:Xanthophylls; D002734:Chlorophyll; D002338:Carotenoids; C037184:chlorophyll b; C011947:neoxanthin