Chemoenzymatic synthesis of L-galactosylated dimeric sialyl Lewis X structures employing alpha-1,3-fucosyltransferase V.
L-Galactosylated dimeric sialyl Lewis X (SLeX) has been prepared employing a combination of chemical and enzymatic synthetic methods. GDP-L-galactose has been chemically synthesised. Enzymatic transfer of L-galactose onto the acceptor (Sia-alpha2,3-Gal-beta1,4-GlcNAc-beta1,3/6)2-Man-alpha1-OMe was achieved using the human alpha-1,3-fucosyltransferase V.
Düffels
A
A
Department of Chemistry, University of Cambridge, UK.
Green
L G
LG
Lenz
R
R
Ley
S V
SV
Vincent
S P
SP
Wong
C H
CH
D002240:Carbohydrate Sequence; D002855:Chromatography, Thin Layer; D005647:Fucosyltransferases / Q000737:chemistry*; D005690:Galactose; D006031:Glycosylation; D006801:Humans; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000138:chemical synthesis*; D000080506:Sialyl Lewis X Antigen
D009844:Oligosaccharides; D000080506:Sialyl Lewis X Antigen; D005647:Fucosyltransferases; D005690:Galactose