A potential mechanism for regulating myosin I binding to membranes in vivo.
Myosin Is are associated with specific membranes, however, the mechanism for regulating their intracellular localization is unclear. As a first step towards understanding this mechanism, membrane rebinding assays using Dictyostelium myoB were performed. Crude, cytosolic myoB bound to intact, but not to NaOH-treated plasma membranes. In contrast, partially purified myoB binds to both intact and NaOH-treated plasma membranes. Chemical cross-linking of cytosolic myoB yielded several products, whereas none were found with the partially purified myoB. These results suggest a model where proteins regulating the specific binding of myoB to the plasma membrane may exist both in the cytosol and on the plasma membrane.
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Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA.
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D000818:Animals; D002462:Cell Membrane / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D004023:Dictyostelium / Q000166:cytology / Q000378:metabolism*; D009218:Myosins / Q000378:metabolism*; D011485:Protein Binding
D009218:Myosins