Induction of active systemic anaphylaxis by oral sensitization with ovalbumin in mast-cell-deficient mice.
Mast-cell-deficient W/W(v) mice were sensitized by oral administration of 0.1 and 1.0 mg ovalbumin (OVA) by gavage every day for 9 weeks, and active systemic anaphylaxis (ASA) was induced by intraperitoneal injection of OVA. The production of OVA-specific IgE and IgG1 by oral immunization of the W/W(v) mice was high, and the production of IL-4 by splenocytes re-stimulated with OVA in vitro was increased. In contrast, production of OVA-specific IgG2a and IgG2b was low, and production of IFN-gamma by splenocytes after re-stimulation with OVA in vitro was rather decreased. These findings suggest that Th2-dominant helper T-cell activation had occurred. No increase in serum histamine level was observed following ASA induction. However, the plasma platelet-activating factor (PAF) levels of the mice sensitized with 0.1 and 1.0 mg OVA by gavage increased significantly. The increases in plasma PAF correlated well with the ASA-associated decreases in body temperature, suggesting that PAF plays an important role in ASA in W/W(v) mice. Taken together the above findings indicate that W/W(v) mice are a good model not only for studying induction of food allergy but also for examining the role of PAF in food-induced hypersensitivity.
Okunuki
H
H
Division of Biochemistry and Immunochemistry, National Institute of Health Sciences, 1-18-1, Kamiyoga, Setagaya-ku, 158-8501, Tokyo, Japan.
Teshima
R
R
Sakushima
J
J
Akiyama
H
H
Goda
Y
Y
Toyoda
M
M
Sawada
J I
JI
D000284:Administration, Oral; D000707:Anaphylaxis / Q000209:etiology*; D000818:Animals; D001835:Body Weight; D004195:Disease Models, Animal; D005260:Female; D005334:Fever / Q000209:etiology; D005512:Food Hypersensitivity / Q000276:immunology*; D006632:Histamine / Q000097:blood; D007274:Injections, Intraperitoneal; D007371:Interferon-gamma / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D008407:Mast Cells / Q000502:physiology*; D051379:Mice; D008817:Mice, Mutant Strains; D009929:Organ Size; D010047:Ovalbumin / Q000008:administration & dosage / Q000633:toxicity*; D010972:Platelet Activating Factor / Q000032:analysis; D018418:Th2 Cells / Q000276:immunology
D010972:Platelet Activating Factor; D015847:Interleukin-4; D006632:Histamine; D007371:Interferon-gamma; D010047:Ovalbumin