title: Direct coupling between meiotic DNA replication and recombination initiation.
abstract: During meiosis in Saccharomyces cerevisiae, DNA replication occurs 1. 5 to 2 hours before recombination initiates by DNA double-strand break formation. We show that replication and recombination initiation are directly linked. Blocking meiotic replication prevented double-strand break formation in a replication-checkpoint-independent manner, and delaying replication of a chromosome segment specifically delayed break formation in that segment. Consequently, the time between replication and break formation was held constant in all regions. We suggest that double-strand break formation occurs as part of a process initiated by DNA replication, which thus determines when meiotic recombination initiates on a regional rather than a cell-wide basis.
authors.affiliation: Laboratory of Biochemistry, Division of Basic Science, National Cancer Institute, Bethesda, MD 20892-4255, USA.
authors.forename: V
authors.initials: V
authors.lastname: Borde
authors.forename: A S
authors.initials: AS
authors.lastname: Goldman
authors.forename: M
authors.initials: M
authors.lastname: Lichten
mesh_terms: D015825:Chromosomes, Fungal / Q000235:genetics / Q000378:metabolism; D004260:DNA Repair; D004261:DNA Replication; D004271:DNA, Fungal / Q000378:metabolism*; D005800:Genes, Fungal; D008540:Meiosis; D009154:Mutation; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism
chemical_list: D004271:DNA, Fungal