[Measurement of reticulated platelet using whole blood: methodology and clinical application].
We established simple and rapid method to measure reticulated platelets using whole blood. After whole blood was fixed with paraformaldehyde, sample was stained with thiazole orange and analyzed using flow cytometer. %RP was significantly higher in patients with idiopathic thrombocytopenic purpura(ITP) (27.7 +/- 14.3%) compared with that of normal subjects(11.9 +/- 3.1%), and elevated in 73% of ITP patients. Furthermore, %RP reflected the status of disease very well in a patient with ITP undergoing therapy. Our method can be effectively applied for differential diagnosis and assessment of the status of thrombocytopenic disorders.
Nishiyama
M
M
Central Laboratory for Clinical Investigation, Osaka University Hospital, Suita.
Hayashi
S
S
Futsukaichi
Y
Y
Suehisa
E
E
Kurata
Y
Y
D000293:Adolescent; D000328:Adult; D000368:Aged; D052160:Benzothiazoles; D001792:Blood Platelets / Q000473:pathology*; D003937:Diagnosis, Differential; D005434:Flow Cytometry; D006801:Humans; D008875:Middle Aged; D010976:Platelet Count / Q000379:methods*; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000097:blood; D011804:Quinolines; D013844:Thiazoles
D052160:Benzothiazoles; D011804:Quinolines; D013844:Thiazoles; C051000:thiazole orange