Improved pulsed-field gel electrophoresis for typing vancomycin-resistant enterococci.
A rapid protocol for subtyping vancomycin-resistant enterococci by pulsed-field gel electrophoresis is reported. The procedure is simple and potentially cost-effective and allows reproducible subtyping of the strains in approximately 1 day.
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Division of Molecular Epidemiology, Department of Epidemiology and Preventive Medicine, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA.
Kotetishvili
M
M
Kreger
A
A
Morris
J G
JG
Sulakvelidze
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A
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D016521:Electrophoresis, Gel, Pulsed-Field; D013293:Enterococcus faecalis / Q000145:classification* / Q000187:drug effects; D016984:Enterococcus faecium / Q000145:classification* / Q000187:drug effects; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology*; D006801:Humans; D015203:Reproducibility of Results; D014640:Vancomycin / Q000494:pharmacology; D020713:Vancomycin Resistance
D000900:Anti-Bacterial Agents; D014640:Vancomycin