title: Cat allergen sampling by a new personal collector (Partrap FA 52).
abstract: Recent studies carried out by us and others have demonstrated that Fel d 1, the main cat allergen, may be passively transferred by human clothing in cat-free environments. Consequently, the monitoring of the Fel d 1 levels either in indoor environments or on allergen-contaminated clothes of sensitized cat owners should be considered an important tool in prevention strategies. The aim of this study was to evaluate the efficacy of a personal air sampler (Partrap FA 52) in capturing cat allergen from wool fabrics. Seven identical wool webs (80 x 100 cm) were put in the baskets of seven male cats for 1 week. In our laboratory each web was divided into two parts (80 x 50 cm), the first of which was then divided in two parts (40 x 50 cm) and each was vacuumed directly by one collector. The second part was dry-cleaned at a professional cleaners, divided in two parts and then vacuumed. For the dust collection from wool webs we used a fixed high volume air sampler (CF/20 Gelaire Flow Labs, Milan, Italy) and a personal collector (Partrap FA 52, Coppa Biella, Italy). Fel d 1 content was determined using a two site ELISA (ALK-Abelló Group, Madrid, Spain). Both air samplers collected cat allergens from cat-exposed wool fabrics before and after dry cleaning. There were significant differences between the levels of Fel d 1 before and after dry cleaning by using either CF/20 or Partrap FA52 and between the levels of Fel d 1 before dry cleaning using CF/20 and Partrap FA 52. The results of our study suggest that Partrap FA 52, although its air flow is half that of the CF/20, is able to collect even residual amounts of cat allergen from wool webs after dry cleaning and consequently may constitute a simple and effective means of monitoring the levels of Fel d 1 on the clothes of cat owners.
authors.affiliation: Department of Chest Diseases, Division of Pneumology and Allergology, Hospital A. Cardarelli, Naples, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Liccardi
authors.forename: M
authors.initials: M
authors.lastname: Russo
authors.forename: D
authors.initials: D
authors.lastname: Barber
authors.forename: C
authors.initials: C
authors.lastname: Califano
authors.forename: S
authors.initials: S
authors.lastname: Parmiani
authors.forename: M
authors.initials: M
authors.lastname: D'Amato
authors.forename: G
authors.initials: G
authors.lastname: D'Amato
mesh_terms: D016902:Air Pollution, Indoor / Q000009:adverse effects / Q000032:analysis*; D000818:Animals; D002415:Cats; D003020:Clothing; D004391:Dust / Q000009:adverse effects / Q000032:analysis; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods; D006023:Glycoproteins / Q000009:adverse effects / Q000032:analysis*; D006801:Humans; D008297:Male
chemical_list: D004391:Dust; D006023:Glycoproteins; C059296:Fel d 1 protein, Felis domesticus