Presence of cloning vector sequences in the untranslated region of some genes in Genbank.
We found multiple cloning site sequences in the reported untranslated regions (UTR) of several genes in Genbank. The erroneous information can result in the failure to amplify DNA fragments containing untranslated regions by RT-PCR. It is suggested that a BLAST search be performed when primers are designed for PCR amplification of the 5' or 3' UTR of genes to ensure that the reported UTR does not contain plasmid-derived sequences.
Liao
K W
KW
Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan.
Chang
Y W
YW
Roffler
S R
SR
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000954:Antigens, Surface; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D016208:Databases, Factual; D005822:Genetic Vectors; D006801:Humans; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000954:Antigens, Surface; D017931:DNA Primers; D009363:Neoplasm Proteins; C074075:TM4SF1 protein, human