Co-induction of heme oxygenase-1 and peroxiredoxin I in astrocytes and microglia around hemorrhagic region in the rat brain.
Heme[none1] oxygenase-1 (HO-1) and peroxiredoxin I (PrxI) are known to be oxidative stress- and heme-related proteins. The antioxidant activity of PrxI is inhibited by heme, therefore co-expression of HO-1 and PrxI is considered to be a reasonable mechanism to maintain its antioxidative function. Immunoblotting demonstrated that HO-1 and PrxI were induced around the hemorrhagic region. Immunohistochemical studies revealed that, in acute phase, HO-1 and PrxI were induced primarily in microglia. In the subacute and chronic phase, the immunoreactivity of HO-1 and PrxI in astrocytes was the most intense. These data are the first to demonstrate co-induction of HO-1 and PrxI in the brain. Our results suggest that HO-1 and PrxI are localized in a similar manner to assure the antioxidant activity of PrxI under stress conditions associated with intracerebral hemorrhage.
Nakaso
K
K
Department of Biochemistry, Faculty of Medicine, Tottori University, Yonago, Japan. kazuhiro@grape.med.tottori-u.ac.jp
Kitayama
M
M
Mizuta
E
E
Fukuda
H
H
Ishii
T
T
Nakashima
K
K
Yamada
K
K
D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D002543:Cerebral Hemorrhage / Q000378:metabolism*; D004790:Enzyme Induction; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism*; D051547:Heme Oxygenase-1; D015151:Immunoblotting; D007150:Immunohistochemistry; D008297:Male; D017628:Microglia / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism*; D054464:Peroxiredoxins; D051381:Rats; D017208:Rats, Wistar
D010544:Peroxidases; D054464:Peroxiredoxins; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1