Column-switching HPLC for the analysis of plasma in PET imaging studies.
A column-switch high performance liquid chromatography method for the analysis of 4 mL of plasma is described with six examples of chromatography of [(11)C]-labeled positron-emission tomography imaging agents. Complete extraction of all but the most polar metabolites by the reverse phase capture column is achieved by disruption of plasma protein binding by 8 M urea.
Hilton
J
J
Department of Radiology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA. jhilton@jhmi.edu
Yokoi
F
F
Dannals
R F
RF
Ravert
H T
HT
Szabo
Z
Z
Wong
D F
DF
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D003042:Cocaine / Q000031:analogs & derivatives* / Q000097:blood; D015259:Dopamine Agents / Q000097:blood; D007093:Imidazoles / Q000097:blood; D007546:Isoquinolines / Q000097:blood; D008024:Ligands; D011725:Pyridines / Q000097:blood; D020891:Raclopride / Q000097:blood; D012680:Sensitivity and Specificity; D012997:Solvents / Q000737:chemistry; D013747:Tetrabenazine / Q000031:analogs & derivatives / Q000097:blood; D013997:Time Factors; D014055:Tomography, Emission-Computed; D014508:Urea / Q000737:chemistry
D015259:Dopamine Agents; D007093:Imidazoles; D007546:Isoquinolines; D008024:Ligands; D011725:Pyridines; D012997:Solvents; C099071:L 159884; C032811:dihydrotetrabenazine; D020891:Raclopride; C021279:(1R-(exo,exo))-3-(4-fluorophenyl)-8-methyl-8- azabicyclo(3.2.1)octane-2-carboxylic acid, methyl ester; D014508:Urea; C058079:McN 5652; D003042:Cocaine; D013747:Tetrabenazine