Hoxa-5 in mouse developing lung: cell-specific expression and retinoic acid regulation.
Hoxa-5 is a homeobox gene that is highly expressed in the developing mouse lung. However, little is known about the molecular mechanisms controlling expression. We characterized the ontogeny of Hoxa-5 gene and protein expressions during lung development and then studied the cell-specific effects of retinoic acid (RA) on Hoxa-5 mRNA in fetal lung fibroblasts and MLE-12 mouse lung epithelial cells. Strong but constant Hoxa-5 gene and protein expressions were detected from mouse lung on embryonic day 13.5 to postnatal day 2. At baseline, the gene was strongly expressed in the fibroblasts of day 17.5 fetal mouse lungs. A very weak but reproducible expression was present in the MLE-12 cells. RA stimulated gene expression in both cell types in a time- and dose-dependent manner. Peak expression occurred much later in the MLE-12 cells compared with that in fibroblasts. Cycloheximide and actinomycin D treatment studies suggested that the differences in RA effect on each cell type may involve the presence of a repressor that can be overcome by RA.
Kim
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Division of Newborn Medicine, Floating Hospital for Children at New England Medical Center, Boston, Massachusetts 02111, USA. ckim@lifespan.org
Nielsen
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D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D002460:Cell Line; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D005314:Embryonic and Fetal Development; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D005865:Gestational Age; D018398:Homeodomain Proteins / Q000235:genetics*; D007700:Kinetics; D008168:Lung / Q000196:embryology / Q000254:growth & development / Q000502:physiology*; D008297:Male; D008715:Methionine / Q000378:metabolism; D051379:Mice; D010750:Phosphoproteins; D020545:Respiratory Mucosa / Q000378:metabolism; D012727:Sex Characteristics; D014157:Transcription Factors; D014158:Transcription, Genetic / Q000187:drug effects*; D014212:Tretinoin / Q000494:pharmacology; D014529:Uridine / Q000378:metabolism
D018398:Homeodomain Proteins; C059637:Hoxa5 protein, mouse; D010750:Phosphoproteins; D014157:Transcription Factors; D003609:Dactinomycin; D014212:Tretinoin; D003513:Cycloheximide; D008715:Methionine; D014529:Uridine