title: Structure of a dioxygen reduction enzyme from Desulfovibrio gigas.
abstract: Desulfovibrio gigas is a strict anaerobe that contains a well-characterized metabolic pathway that enables it to survive transient contacts with oxygen. The terminal enzyme in this pathway, rubredoxin:oxygen oxidoreductase (ROO) reduces oxygen to water in a direct and safe way. The 2.5 A resolution crystal structure of ROO shows that each monomer of this homodimeric enzyme consists of a novel combination of two domains, a flavodoxin-like domain and a Zn-beta-lactamase-like domain that contains a di-iron center for dioxygen reduction. This is the first structure of a member of a superfamily of enzymes widespread in strict and facultative anaerobes, indicating its broad physiological significance.
authors.affiliation: Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. da República, Apartado 127, 2781-901 Oeiras, Portugal.
authors.forename: C
authors.initials: C
authors.lastname: Frazão
authors.forename: G
authors.initials: G
authors.lastname: Silva
authors.forename: C M
authors.initials: CM
authors.lastname: Gomes
authors.forename: P
authors.initials: P
authors.lastname: Matias
authors.forename: R
authors.initials: R
authors.lastname: Coelho
authors.forename: L
authors.initials: L
authors.lastname: Sieker
authors.forename: S
authors.initials: S
authors.lastname: Macedo
authors.forename: M Y
authors.initials: MY
authors.lastname: Liu
authors.forename: S
authors.initials: S
authors.lastname: Oliveira
authors.forename: M
authors.initials: M
authors.lastname: Teixeira
authors.forename: A V
authors.initials: AV
authors.lastname: Xavier
authors.forename: C
authors.initials: C
authors.lastname: Rodrigues-Pousada
authors.forename: M A
authors.initials: MA
authors.lastname: Carrondo
authors.forename: J
authors.initials: J
authors.lastname: Le Gall
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D018360:Crystallography, X-Ray; D003901:Desulfovibrio / Q000201:enzymology* / Q000235:genetics; D019281:Dimerization; D005418:Flavodoxin / Q000737:chemistry; D007501:Iron / Q000378:metabolism; D008024:Ligands; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D014867:Water / Q000378:metabolism; D001618:beta-Lactamases / Q000737:chemistry
chemical_list: D005418:Flavodoxin; D008024:Ligands; D011994:Recombinant Proteins; D014867:Water; D007501:Iron; D010088:Oxidoreductases; C081469:rubredoxin-oxygen oxidoreductase; D001618:beta-Lactamases; D010100:Oxygen