title: Hepatic microvascular features in experimental cirrhosis: a structural and morphometrical study in CCl4-treated rats.
abstract: OBJECTIVE
  In this study, a detailed morphometrical analysis of the hepatic microvasculature in the different zones of hepatic parenchyma was performed in normal and cirrhotic rat liver (CCl4-induced). The aims were to detect, in CCl4-induced cirrhosis, the real presence of the "capillarization" of hepatic sinusoids and to assess alterations of the sinusoid/parenchyma ratio within the nodule.
  
  
  METHODS
  Cirrhosis was promoted by controlled intragastric CCl4 administration. Scanning electron microscopy of the vascular corrosion cast technique associated with light microscopy and transmission electron microscopy were used.
  
  
  RESULTS
  Evidence of connective tissue in the space of Disse was found only in sinusoids located near portal tracts or large fibrotic areas, and this was also confirmed by laminin immunohistochemistry. In contrast, all the intranodular sinusoids lacked real basal membrane and connective fibers in the space of Disse and, displayed normal fenestrations. The parenchymal area, sinusoidal area, mean sinusoidal area, sinusoidal perimeter, hepatocyte area and the reciprocal ratios were all considered in the morphometrical analysis. The sinusoids were of uniform size in the periportal, periseptal and pericentral areas of the cirrhotic liver without the typical zonal differences of the normal liver. The areas occupied by sinusoids per unit of parenchyma and the sinusoid/hepatocyte interfaces disposable for metabolic exchanges were markedly smaller (p<0.01) in cirrhotic than normal liver.
  
  
  CONCLUSIONS
  Our findings indicate that capillarization of hepatic sinusoids occurs only in very limited regions of the cirrhotic parenchyma, and thus this phenomenon does not have relevant functional consequences. Furthermore, the cirrhotic parenchyma appears not to be supplied by sinusoids and lacks features of zonation, which is a condition that could play a major role in the development and progression of liver failure.
authors.affiliation: Department of Experimental Medicine, State University of L'Aquila, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Onori
authors.forename: S
authors.initials: S
authors.lastname: Morini
authors.forename: A
authors.initials: A
authors.lastname: Franchitto
authors.forename: R
authors.initials: R
authors.lastname: Sferra
authors.forename: D
authors.initials: D
authors.lastname: Alvaro
authors.forename: E
authors.initials: E
authors.lastname: Gaudio
mesh_terms: D000818:Animals; D002251:Carbon Tetrachloride / Q000633:toxicity*; D003238:Connective Tissue / Q000473:pathology / Q000648:ultrastructure; D007091:Image Processing, Computer-Assisted; D008099:Liver / Q000098:blood supply* / Q000473:pathology / Q000648:ultrastructure; D008102:Liver Circulation / Q000187:drug effects*; D008106:Liver Cirrhosis, Experimental / Q000139:chemically induced / Q000473:pathology*; D008297:Male; D008833:Microcirculation / Q000187:drug effects / Q000473:pathology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002251:Carbon Tetrachloride