Effect of a histamine H1 receptor antagonist on gastric endocrine cell proliferation induced by chronic acid suppression in rats.
The effect of histamine on gastrin cells and enterochromaffin-like cells has not yet been clarified. We investigated the influence of pyrilamine (a histamine H1 receptor antagonist) on serum gastrin level, gastrin cells, and enterochromaffin-like cells in rats with or without 4 weeks of famotidine treatment. The rats were divided into six groups: a control group, two pyrilamine groups (2mg/kg, or 20mg/kg, p.o.), a famotidine group (20mg/kg twice/daily i.m.), and two pyrilamine + famotidine groups. The serum gastrin concentration was determined, and gastrin cells and enterochromaffin-like cells were identified by the labeled streptavidin biotin complex method and counted. Hypergastrinemia, gastrin cell hyperplasia, and enterochromaffin-like cell hyperplasia were found after 4 weeks of famotidine treatment. Four weeks of treatment with pyrilamine alone did not affect the gastrin level, gastrin cells, or enterochromaffin-like cells in the rat stomach. When combined with famotidine, pyrilamine enhanced famotidine-induced hypergastrinemia, but it did not affect gastrin cell hyperplasia, and it significantly inhibited enterochromaffin-like cell hyperplasia. These results suggest that gastrin secretion and enterochromaffin-like cell proliferation may be regulated by histamine via the H1 receptor during acid suppression.
Chen
G
G
Department of Surgery (II), The Jikei University School of Medicine, Tokyo, Japan.
Kashiwagi
H
H
Omura
N
N
Aoki
T
T
D000818:Animals; D019861:Enterochromaffin-like Cells / Q000166:cytology / Q000187:drug effects*; D015738:Famotidine / Q000494:pharmacology*; D005744:Gastric Acid / Q000378:metabolism*; D019863:Gastrin-Secreting Cells / Q000166:cytology / Q000187:drug effects*; D005755:Gastrins / Q000097:blood*; D006634:Histamine H1 Antagonists / Q000494:pharmacology*; D006965:Hyperplasia; D007150:Immunohistochemistry; D008297:Male; D011738:Pyrilamine / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D005755:Gastrins; D006634:Histamine H1 Antagonists; D015738:Famotidine; D011738:Pyrilamine