[Possibilities for standardization of ELISA for detection of Salmonella antibodies in sera and meat juices of pigs].
Programmes for controlling salmonella infections in German piggeries are based on the meat-juice-ELISA conducted in various investigation centres by using different test-kits. A usual procedure for harmonization (standardisation) of results is the calculation of the percentage of antibody-concentration from field samples in relation to the extinctions of a set of control-sera with known antibody concentrations. Whether this system is still acceptable in case of using different test-kits seems to be questionable. In principle, difficulties arise by calculating field results from the regression curve of control-sera because the calculated percentages of antibodies do not represent the antibody concentration but, instead, the percentages of the extinctions measured, and secondly, because control-sera presently in use are directed against different salmonella serovars. In regard to the number of laboratories involved and because of a variety of test-kits used it seems to be more adequate to include only one anti-Salmonella Typhimurium standard-serum at a given antibody concentration which is to be tested repeatedly on every test-plate. Simultaneously, further controls should include another anti-Salmonella Typhimurium and one anti-Salmonella Choleraesuis serum which should provide results similar to the Danish system which is regarded as a standard. As well, a negative serum must be included in the test and a minimum difference in extinctions between this negative serum and the standard positive control-serum should be reached to prove the validity of results from the test plate.
Steinbach
G
G
Bundesinstitut für gesundheitlichen Verbraucherschutz und Veterinärmedizin, Berlin.
Staak
C
C
Bahn
P
P
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis* / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards / Q000662:veterinary*; D008460:Meat / Q000382:microbiology*; D012481:Salmonella Infections, Animal / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D012486:Salmonella typhimurium / Q000276:immunology*; D013552:Swine; D013553:Swine Diseases / Q000097:blood / Q000175:diagnosis* / Q000276:immunology
D000907:Antibodies, Bacterial