Characterization of bovine and human lactoferrins as glycyrrhizin-binding proteins and their phosphorylation in vitro by casein kinase II.
The binding ability of bovine and human lactoferrins (bLF and hLF; LFs) to a glycyrrhizin (GL)-affinity column and their phosphorylation by casein kinase II (CK-II) in vitro were biochemically investigated. It was found that (i) both bLF and hLF are GL-binding proteins; (ii) purified both proteins function as phosphate acceptors of CK-II; and (iii) this phosphorylation is completely inhibited by two polyphenol-containing anti-oxidant compounds (quercetin and epigallocatechin gallate) at I microm, whereas a glycyrrhetinic acid derivative (oGA) inhibits it at one tenth the concentration of GL. The DNA-binding affinity of hLF was reduced by GL in a dose dependent manner. However, no significant effect of the CK-II-mediated hLF phosphorylation on its DNA-binding affinity was detected. These results suggest that the GL-induced inhibition of the DNA-binding affinity and the CK-II-mediated phosphorylation of hLF may be closely correlated with the anti-inflammatory effect of GL in the human body.
Hatomi
M
M
Laboratory of Genetical Biochemistry, Kitasato University, Sagamihara, Japan.
Tanigawa
K
K
Fujihara
M
M
Ito
J
J
Yanahira
S
S
Ohtsuki
K
K
D000818:Animals; D001345:Autoradiography; D002352:Carrier Proteins / Q000737:chemistry* / Q000302:isolation & purification; D047390:Casein Kinase II; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D006152:Cyclic GMP / Q000378:metabolism; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007700:Kinetics; D007781:Lactoferrin / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011794:Quercetin / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology
D002352:Carrier Proteins; D004268:DNA-Binding Proteins; C490098:LTF protein, human; D011994:Recombinant Proteins; C086010:glycyrrhizin-binding proteins; D004247:DNA; D011794:Quercetin; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases; D007781:Lactoferrin; D006152:Cyclic GMP