In vivo study of tumor metabolism: an application of new multi-probe microdialysis system in the striatum of freely moving rats grafted with C6 cells.
The purpose of this study is to investigate the in vivo tumoral brain metabolism in free moving rats using microdialysis. Cells from C6 glioma cell line were inoculated in one striatum 15 days before the microdialysis experimentation. Then, using a new system allowing perfusion of several microdialysis probes in free moving rat, normalised dialysate levels of glucose, lactate and pyruvate were monitored in both glioma and control striatum. At the end of the procedure, animals were sacrificed for histological study. Data shows that probe functioning is similar in both tissues. The results for normalised glucose level were in striatum control: 2.14 mM, in tumoral striatum: 1.71 mM (P>0.1); for lactate, respectively, 0.86 and 1.65 mM (P<0.05) and for pyruvate, respectively, 65.56 and 140. 94 microM (P<0.05). This data clearly shows a significant increase of pyruvate and lactate in tumoral striatum compared to normal striatum, correlating previous in vitro studies on glioma metabolism. We conclude that this microdialysis technique is of value in tumoral brain and could constitute an interesting tool for a better understanding of glioma metabolism.
Darbin
O
O
Université de la Méditerranée, Laboratoire de barobiologie et de neurochimie des interactions cellulaires, Institut J. Roche, Faculté de médecine Nord. 13916 Cedex 20, Marseille, France.
Lonjon
M
M
Quentien
M H
MH
Michiels
J F
JF
Grellier
P
P
Negrin
J
J
Rostain
J C
JC
Risso
J J
JJ
D000818:Animals; D001932:Brain Neoplasms / Q000378:metabolism*; D003342:Corpus Striatum / Q000378:metabolism*; D005910:Glioma / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D019344:Lactic Acid / Q000378:metabolism*; D008297:Male; D017551:Microdialysis / Q000379:methods*; D019289:Pyruvic Acid / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014407:Tumor Cells, Cultured
D019344:Lactic Acid; D019289:Pyruvic Acid; D005947:Glucose