[Isolation of DNA from museum exhibits of butterflies (Lepidoptera, Papilionidae) and PCR analysis with random and universal genes-specific primers].
The effect of the duration of storage of entomological material on DNA preservation was estimated. The results of the optimization of conditions for the analysis of random amplified polymorphic DNA in a polymerase chain reaction (RAPD-PCR) are presented as applied to the DNA of lepidopterans of the family Papilionidae. RAPD patterns are shown for the first time in Atrophaneura alcinous and four species of the genus Parnassius (sensu lata). The applicability of museum specimens of butterflies for RAPD analysis was demonstrated. The results of PCR analysis using DNA obtained from different collection specimens stored for up to five years were compared. The authenticity of DNA obtained from collection specimens was proved using PCR with universal primers, which are specific to the COI and COII cytochrome genes of mitochondrial DNA (mt DNA). The lengths of individuals gene fragments obtained by the amplification of both museum and live specimens were 800 and 1600 bp. The conservative regions of mitochondrial genome were shown to be slightly different in two A. alcinous subspecies.
Zakharov
E V
EV
Institute of Biology and Soil Sciences, Russian Academy of Sciences, Vladivostok, Russia.
Chelomina
G N
GN
Zhuravlev
Iu N
IuN
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000302:isolation & purification*; D017931:DNA Primers; D003576:Electron Transport Complex IV / Q000235:genetics; D007527:Isoenzymes / Q000235:genetics; D007915:Lepidoptera / Q000235:genetics*; D009144:Museums; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique
D017931:DNA Primers; D007527:Isoenzymes; D004247:DNA; D003576:Electron Transport Complex IV