title: Increase of reactive oxygen (ROS) and nitrogen (RNS) species generated by phagocyting granulocytes related to age.
abstract: In this present paper the age-induced effect on reactive oxidizing species generated by oxygen (ROS) and nitrogen (RNS) was studied using human phagocyting granulocytes. The ROS and RNS were quantified, respectively, in a chemiluminescence assay and by the measurement of nitrite production. The age-induced reactive oxidizing species generation was studied in healthy subjects ranging from 20 to 80 years old, divided into six age groups: group I, 20-29 years old; group II, 30-39 years old; group III, 40-49 years old; group IV, 50-59 years old; group V, 60-69 years old; and group VI, 70-80 years old. Our results demonstrate a parallelism between generation of the ROS and RNS induced by the age. A significant increase of ROS production was observed from 40 years old (age groups III, IV, V and VI while for RNS this increase was observed only from 50 years old (groups IV, V and VI). These data suggest an increase of oxidizing species generation (ROS/RNS) related to age. The increased generation of ROS (40-49 years old) was induced before the increasing of RNS (50-59 years old) and it may have consequences on inflammation and host defences.
authors.affiliation: Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627 CP 486, 30161-970, Minas Gerais, Belo Horizonte, Brazil. chavesmm@mono.icb.ufmg.br
authors.forename: M
authors.initials: M
authors.lastname: Martins Chaves
authors.forename: E
authors.initials: E
authors.lastname: Rocha-Vieira
authors.forename: A
authors.initials: A
authors.lastname: Pereira dos Reis
authors.forename: R
authors.initials: R
authors.lastname: de Lima e Silva
authors.forename: N C
authors.initials: NC
authors.lastname: Gerzstein
authors.forename: J A
authors.initials: JA
authors.lastname: Nogueira-Machado
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism*; D005260:Female; D006098:Granulocytes / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009573:Nitrites / Q000378:metabolism*; D010587:Phagocytosis; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D009573:Nitrites; D017382:Reactive Oxygen Species