Molecular dynamics of the anticodon domain of yeast tRNA(Phe): codon-anticodon interaction.
We have studied the effect of codon-anticodon interaction on the structure and dynamics of transfer RNAs using molecular dynamics simulations over a nanosecond time scale. From our molecular dynamical investigations of the solvated anticodon domain of yeast tRNA(Phe) in the presence and absence of the codon trinucleotides UUC and UUU, we find that, although at a gross level the structures are quite similar for the free and the bound domains, there are small but distinct differences in certain parts of the molecule, notably near the Y37 base. Comparison of the dynamics in terms of interatomic or inter-residual distance fluctuation for the free and the bound domains showed regions of enhanced rigidity in the loop region in the presence of codons. Because fluorescence experiments suggested the existence of multiple conformers of the anticodon domain, which interconvert on a much larger time scale than our simulations, we probed the conformational space using five independent trajectories of 500 ps duration. A generalized ergodic measure analysis of the trajectories revealed that at least for this time scale, all the trajectories populated separate parts of the conformational space, indicating a need for even longer simulations or enhanced sampling of the conformational space to give an unequivocal answer to this question.
Lahiri
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A
Center for Structural Biochemistry, Karolinska Institutet, S 141 57 Huddinge, Sweden.
Nilsson
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L
D000926:Anticodon / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D055592:Biophysical Phenomena; D001703:Biophysics; D003062:Codon / Q000737:chemistry / Q000235:genetics; D006860:Hydrogen Bonding; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D012331:RNA, Fungal / Q000737:chemistry* / Q000235:genetics*; D012360:RNA, Transfer, Phe / Q000737:chemistry* / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000235:genetics; D013816:Thermodynamics
D000926:Anticodon; D003062:Codon; D012331:RNA, Fungal; D012360:RNA, Transfer, Phe