title: Role of nitric oxide in hypoxia-induced changes in newborn rats.
abstract: In order to investigate the role of nitric oxide (NO) in hypoxic tissue damage in newborns, we studied the effects of systemic administration of an inhibitor of NO synthase, N(G)-nitro-L-arginine (L-NNA), and the precursor for the synthesis of NO, L-arginine (L-ARG), on the biochemical and histological changes in brain, heart, lung, liver, kidney, intestine, and skeletal muscle tissues. Four groups of 1-day-old Wistar rat pups were used: control, hypoxic, L-ARG, and L-NNA groups. L-ARG 100 mg/kg or L-NNA 2 mg/kg was administered as a bolus intraperitoneally 1.5 h before hypoxia. Hypoxia increased lipid peroxidation in all tissues except muscle; this increase was prevented by L-NNA and L-ARG in brain, heart, lung, kidney, and liver tissues. L-NNA in intestine and L-ARG in muscle tissue increased lipid peroxidation. The tissue-associated myeloperoxidase activity was decreased in the liver by L-NNA and L-ARG. Histopathological changes in intestines were villous epithelial separation and hyperemia in hypoxic and L-NNA groups which were not observed in control and L-ARG groups. In lungs, pulmonary hemorrhage was observed only in the hypoxic group. These data suggest that NO acts both as a destructive and a protective agent in the pathogenesis of hypoxia-reoxygenation injuries.
authors.affiliation: Department of Pediatrics, Pamukkale University Faculty of Medicine, Denizli, Turkey. iakilic@superonline.com
authors.forename: I
authors.initials: I
authors.lastname: Kiliç
authors.forename: B A
authors.initials: BA
authors.lastname: Kiliç
authors.forename: C
authors.initials: C
authors.lastname: Güven
authors.forename: E
authors.initials: E
authors.lastname: Demirpençe
authors.forename: M A
authors.initials: MA
authors.lastname: Akşit
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism / Q000502:physiology*; D001120:Arginine / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D000860:Hypoxia / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000473:pathology; D008054:Lipid Peroxides / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D008168:Lung / Q000473:pathology; D018482:Muscle, Skeletal / Q000378:metabolism; D009569:Nitric Oxide / Q000502:physiology*; D019335:Nitroarginine / Q000494:pharmacology; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D008054:Lipid Peroxides; D017392:Thiobarbituric Acid Reactive Substances; D019335:Nitroarginine; D009569:Nitric Oxide; D001120:Arginine; D009195:Peroxidase