title: Increase of the isoprostane 8-isoprostaglandin f2alpha in maternal and fetal blood of rats with streptozotocin-induced diabetes: evidence of lipid peroxidation.
abstract: OBJECTIVE
  Pregnancy complicated by diabetes is associated with maternal complications and fetal abnormalities. Animal models of diabetes suggest that heightened free radical production may be implicated in the pathogenesis of this condition. The purpose of this investigation was to evaluate oxidative stress in plasma from diabetic rats and their fetuses through measurement of concentrations of 8-isoprostaglandin F(2alpha), a stable marker of lipid peroxidation.
  
  
  METHODS
  Diabetes was induced in virgin and pregnant rats with streptozotocin. Blood samples were collected after 20 days of diabetes. Adult and fetal plasma 8-isoprostaglandin F(2alpha) concentrations were determined by gas chromatography-mass spectroscopy.
  
  
  RESULTS
  Significantly higher plasma 8-isoprostaglandin F(2alpha) concentrations were observed in the virgin rats with diabetes and in both the pregnant dams with diabetes and their fetuses when compared with their respective control groups without diabetes (P <.001).
  
  
  CONCLUSIONS
  Oxidative stress was induced in both mother and fetus in rodent pregnancy complicated by diabetes. This finding may have implications for fetal dysmorphogenesis and in fetal programming for adulthood disease.
authors.affiliation: Department of Obstetrics and Gynaecology, Guy's, King's and St Thomas' School of Medicine, London, United Kingdom, USA.
authors.forename: R T
authors.initials: RT
authors.lastname: Gerber
authors.forename: K
authors.initials: K
authors.lastname: Holemans
authors.forename: I
authors.initials: I
authors.lastname: O'Brien-Coker
authors.forename: A I
authors.initials: AI
authors.lastname: Mallet
authors.forename: R
authors.initials: R
authors.lastname: van Bree
authors.forename: F A
authors.initials: FA
authors.lastname: Van Assche
authors.forename: L
authors.initials: L
authors.lastname: Poston
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D003921:Diabetes Mellitus, Experimental / Q000097:blood* / Q000378:metabolism; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000097:blood*; D028441:F2-Isoprostanes; D005260:Female; D005312:Fetal Blood; D008054:Lipid Peroxides / Q000378:metabolism; D009994:Osmolar Concentration; D011247:Pregnancy; D011248:Pregnancy Complications / Q000097:blood*; D011270:Pregnancy, Animal / Q000097:blood*; D051381:Rats; D012016:Reference Values
chemical_list: D001786:Blood Glucose; D028441:F2-Isoprostanes; D008054:Lipid Peroxides; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost