Nuclear envelope fission is linked to cytokinesis in budding yeast.
We have investigated the relationship between nuclear envelope fission and cytokinesis during mitotic cell division in budding yeast. By carrying out time-lapse and optical sectioning video microscopy analysis of cells that express green fluorescent protein (GFP)-tagged nuclear envelope and actomyosin ring components, we found that nuclear division is temporally coupled to cytokinesis. Light and electron microscopy analysis also showed that nuclear envelope fission and the division of the nucleoplasm are severely delayed in cytokinesis mutants, resulting in discoupling between the nuclear division cycle and the budding cycle. These results suggest that homotypic membrane fusion may be activated by components or the mechanical action of cytokinetic structures and presents a mechanism for the equal partitioning of the nucleus and the temporal coordination of this event with chromosome segregation during mitosis.
Lippincott
J
J
Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts, 02115, USA.
Li
R
R
D002455:Cell Division; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D006360:Heat-Shock Proteins; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D026901:Membrane Transport Proteins; D008840:Microfilament Proteins; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism; D009685:Nuclear Envelope / Q000502:physiology*; D019913:Nuclear Localization Signals; D028861:Nuclear Pore Complex Proteins; D009687:Nuclear Proteins / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins; D020703:ras GTPase-Activating Proteins
D005656:Fungal Proteins; C116847:HOF1 protein, S cerevisiae; D006360:Heat-Shock Proteins; C101203:IQ motif containing GTPase activating protein 1; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D008840:Microfilament Proteins; D008869:Microtubule-Associated Proteins; C077727:NUP49 protein, S cerevisiae; D019913:Nuclear Localization Signals; D028861:Nuclear Pore Complex Proteins; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; C074215:SEC63 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D020703:ras GTPase-Activating Proteins