title: Bovine CAPN1 maps to a region of BTA29 containing a quantitative trait locus for meat tenderness.
abstract: Micromolar calcium activated neural protease (CAPN1) was investigated as a potential candidate gene for a quantitative trait locus (QTL) on BTA29 affecting meat tenderness. A 2,948-bp bovine cDNA containing the entire coding region of the gene was obtained, showing 91% identity to human CAPN1. The 716 AA protein predicted from this sequence shows 97% similarity (95% identity) to the 714 AA human protein. Analysis of the gene structure revealed that CAPN1 mRNA is encoded by at least 19 exons, and 11,055 bp of the gene were sequenced, including 17 introns. Two single nucleotide polymorphisms (SNP) were detected in intron 12 and were used to map bovine CAPN1 to the telomeric end of the BTA29 linkage group. This approximately coincides with the position of the QTL, demonstrating that CAPN1 protease is a positional candidate gene potentially affecting variation in meat tenderness in a bovine resource mapping population.
authors.affiliation: USDA, ARS, U.S. Meat Animal Research Center, Clay Center, NE 68933, USA. smith@email.marc.usda.gov
authors.forename: T P
authors.initials: TP
authors.lastname: Smith
authors.forename: E
authors.initials: E
authors.lastname: Casas
authors.forename: C E
authors.initials: CE
authors.lastname: Rexroad
authors.forename: S M
authors.initials: SM
authors.lastname: Kappes
authors.forename: J W
authors.initials: JW
authors.lastname: Keele
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002154:Calpain / Q000235:genetics*; D002417:Cattle / Q000235:genetics*; D002871:Chromosome Banding; D002874:Chromosome Mapping / Q000662:veterinary*; D006801:Humans; D008460:Meat / Q000592:standards*; D008969:Molecular Sequence Data; D019655:Quantitative Trait, Heritable
chemical_list: D002154:Calpain; C484807:CAPN1 protein, Bos taurus; C484806:CAPN1 protein, human