Specific ganglioside changes in extraneural tissues of adult rats with hypothyroidism.
Adults rats with hypothyroidism were prepared by administration of 6-propyl-2-thiouracil (PTU) or methimazole, and the tissues were examined for their gangliosides through methods including glycolipid-overlay techniques. Normal thyroid tissue contained GM3, GD3, and GD1a as the major gangliosides, with GM1, GD1b, GT1b, and GQ1b in lesser amounts. The goitrous tissue of PTU-induced hypothyroid rats had higher concentrations of GM1 and GD1a with a concomitant decrease of GM3. The amount of GT3 in thyroid tissue was increased in hypothyroid animals. While normal liver tissue had a complex ganglioside pattern with a- and b-series gangliosides, the PTU-induced hypothyroid tissue showed a simpler ganglioside profile that consisted mainly of a-series gangliosides with almost undetectable amounts of b-series gangliosides. The expression of c-series gangliosides was suppressed in the hypothyroid liver tissue. Heart tissue had higher contents of GM3 and GT3 than control. No apparent change was observed in the compositions of major and c-series gangliosides in other extraneural tissues (i.e., kidney, lung, spleen, thymus, pancreas, testis, skeletal muscle, and eye lenses), and neural tissues (i.e., cerebrum and cerebellum) from PTU-induced hypothyroid rats. The ganglioside changes of thyroid, liver, and heart tissues were reproduced in corresponding tissues of methimazole-induced hypothyroid rats. These results suggest that hypothyroid conditions affect the biosynthesis and expression of gangliosides in specific tissue and cell types.
Saito
M
M
Department of Clinical Pharmacology and Therapeutics, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, 422-8526, Shizuoka, Japan. saitom@ys2.u-shizuoka-ken.ac.jp
Sugiyama
K
K
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D005732:Gangliosides / Q000378:metabolism*; D006321:Heart / Q000187:drug effects; D007037:Hypothyroidism / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008713:Methimazole / Q000494:pharmacology*; D009206:Myocardium / Q000378:metabolism*; D011441:Propylthiouracil / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D013961:Thyroid Gland / Q000187:drug effects / Q000378:metabolism*; D013974:Thyroxine / Q000097:blood
D005732:Gangliosides; D008713:Methimazole; D011441:Propylthiouracil; D013974:Thyroxine