Crystallization and preliminary X-ray analysis of glucose dehydrogenase from Bacillus megaterium IWG3.
Glucose dehydrogenase from Bacillus megaterium IWG3 has been crystallized in the presence of NAD(+) using the hanging-drop vapour-diffusion method with PEG 2000 as the precipitant. Crystals belong to space group C2 and have unit-cell parameters a = 120.8 (1), b = 66.7 (1), c = 119.6 (1) A, beta = 93.25 (3) degrees with standard deviations in parentheses. Assumption of four subunits in the asymmetric unit gave the most probable Matthews coefficient V(M) of 2.1 A(3) Da(-1) (solvent content 41.7% by volume). X-ray diffraction data were collected to 1.7 A on a synchrotron-radiation source.
Yamamoto
K
K
Department of Chemistry, Nara Medical University, Kashihara, Nara 634-8521, Japan. kama@naramed-u.ac.jp
Kusunoki
M
M
Urabe
I
I
Tabata
S
S
Osaki
S
S
D001410:Bacillus megaterium / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D042843:Glucose 1-Dehydrogenase; D005948:Glucose Dehydrogenases / Q000737:chemistry*; D011487:Protein Conformation
D005948:Glucose Dehydrogenases; D042843:Glucose 1-Dehydrogenase