Serotonin induces apoptosis in PGT-beta pineal gland tumor cells.
Serotonin-induced neuronal cell death has been implicated as a possible cause of neurodegenerative and neuropsychiatric disorders. To investigate the involvement of serotonin-induced apoptosis as a potential mechanism in the pathophysiology of serotonin-related diseases, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, DAPI (4',6-diamidino-2-phenylindole) staining, TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling) assay and flow cytometric analysis were performed using the immortalized pineal cell line PGT-beta. Through morphological and biochemical analyses, it was demonstrated that cell death induced by serotonin in PGT-beta cells shows classic apoptotic features. These data suggest that serotonin induces apoptosis in PGT-beta cells.
Yim
S V
SV
Department of Pharmacology, College of Medicine, Kangwon National University, Hyoja-Dong, Chuncheon, Korea.
Kim
K H
KH
Kim
C J
CJ
Chung
J H
JH
D000818:Animals; D017209:Apoptosis; D005434:Flow Cytometry; D020287:In Situ Nick-End Labeling; D051379:Mice; D008822:Mice, Transgenic; D018377:Neurotransmitter Agents / Q000494:pharmacology; D010871:Pinealoma; D012701:Serotonin / Q000494:pharmacology*; D013194:Staining and Labeling; D014407:Tumor Cells, Cultured
D018377:Neurotransmitter Agents; D012701:Serotonin