title: Cultured human mast cells derived from umbilical cord blood cells in the presence of stem cell factor and interleukin-6 cannot be a model of human skin mast cells: fluorescence microscopic analysis of intracellular calcium ion mobilization.
abstract: To know whether cultured human mast cells raised from umbilical cord blood cells in the presence of stem cell factor (SCF) and interleukin-6 (IL-6) can be a model of human skin mast cells, the cells were stimulated, and intracellular calcium ion ([Ca(2+)](i)) mobilization was analyzed by fluorescence microscopic techniques in parallel with a measurement of histamine released from the cells. When IgE-sensitized mast cells were activated by anti-IgE, [Ca(2+)](i) elevation began at the periphery and subsequently proceeded toward the center of the cells. The increase in [Ca(2+)](i) in calcium ionophore A23187-stimulated mast cells began at the center and spread to the periphery of the cells. Significant histamine release was observed by each stimulation. However, either compound 48/80 or substance P failed to increase [Ca(2+)](i) with no appreciable histamine release. This study shows that there is heterogeneity of [Ca(2+)](i) mobilization in the activated human mast cells, and that cultured human mast cells derived from umbilical cord blood cells in the presence of SCF and IL-6 can not be a model of human skin mast cells.
authors.affiliation: Department of Dermatology, Gunma University School of Medicine, 3-39-22 Showa-machi, 371-8511, Maebashi, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Amano
authors.forename: M
authors.initials: M
authors.lastname: Kurosawa
authors.forename: O
authors.initials: O
authors.lastname: Ishikawa
authors.forename: J
authors.initials: J
authors.lastname: Chihara
authors.forename: Y
authors.initials: Y
authors.lastname: Miyachi
mesh_terms: D000888:Antibodies, Anti-Idiotypic / Q000494:pharmacology; D001692:Biological Transport; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D015850:Interleukin-6 / Q000494:pharmacology*; D007425:Intracellular Membranes / Q000378:metabolism*; D007476:Ionophores / Q000494:pharmacology; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D012867:Skin / Q000166:cytology*; D019089:Stem Cell Factor / Q000494:pharmacology*; D013373:Substance P / Q000494:pharmacology; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
chemical_list: D000888:Antibodies, Anti-Idiotypic; D015850:Interleukin-6; D007476:Ionophores; D019089:Stem Cell Factor; D013373:Substance P; D007073:Immunoglobulin E; D000001:Calcimycin; D003189:p-Methoxy-N-methylphenethylamine; D002118:Calcium