title: Strategies for cell permeabilization and fixation in detecting surface and intracellular antigens.
authors.affiliation: Beckman Coulter, Inc., Miami, Florida 33196, USA.
authors.forename: S K
authors.initials: SK
authors.lastname: Koester
authors.forename: W E
authors.initials: WE
authors.lastname: Bolton
mesh_terms: D000906:Antibodies / Q000737:chemistry / Q000378:metabolism; D000941:Antigens / Q000032:analysis* / Q000737:chemistry; D002460:Cell Line; D002463:Cell Membrane Permeability; D003432:Cross-Linking Reagents / Q000737:chemistry; D003902:Detergents / Q000737:chemistry; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes; D016591:Histocytological Preparation Techniques / Q000379:methods*; D006801:Humans; D013048:Specimen Handling; D016707:Tissue Fixation / Q000379:methods*
chemical_list: D000906:Antibodies; D000941:Antigens; D003432:Cross-Linking Reagents; D003902:Detergents; D005456:Fluorescent Dyes