A transposon carrying the gene mphB for macrolide 2'-phosphotransferase II.
The mphB gene for macrolide 2'-phosphotransferase II is located on two plasmids, pTZ3721 and pTZ3723, in Escherichia coli BM2506. We examined translocation of mphB that originated from pTZ3721. The transposable element carrying mphB is 39 kb long and has a Tn21-like transposition module at one end and a Tn1721-like transposition module at other. The structure of the transposition modules of this element resembles that of Tn2610. However, the gene arrangement of the internal region on the transposon carrying mphB was reverse to that of Tn2610. The nucleotide sequences of both terminal regions suggested that the inversion of the DNA fragment occurred between the res sites by resolvase-mediated recombination.
Noguchi
N
N
Department of Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, 192-0392, Tokyo, Japan. noguchin@ps.toyaku.ac.jp
Katayama
J
J
Sasatsu
M
M
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics*; D010957:Plasmids; D014157:Transcription Factors / Q000235:genetics; D014178:Translocation, Genetic; D045564:Transposon Resolvases
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D029968:Escherichia coli Proteins; C122137:TnpC protein, Pseudomonas putida; D014157:Transcription Factors; D017853:Phosphotransferases (Alcohol Group Acceptor); C061369:macrolide 2'-kinase; C501557:mphB protein, E coli; C473490:Tn21 resolvase; D045564:Transposon Resolvases