Arbitrarily primed PCR fingerprinting of RNA and DNA in Entamoeba histolytica.
Differences were detected in the gene expression of strains of E. histolytica using RNA (RAP-PCR) and DNA fingerprinting (RAPD). Analysis of the electrophoretic profiles of the gels revealed some polymorphic markers that could be used in the individual characterization of the strains. The 260 bands generated by using five different primers for RAP-PCR, as well as RAPD, were employed in the construction of dendograms. The dendogram obtained based on the RAPD products permitted the distinction of symptomatic and asymptomatic isolates, as well the correlation between the polymorphism exhibited and the virulence of the strains. The dendogram obtained for the RAP-PCR products did not show a correlation with the virulence of the strains but revealed a high degree of intraspecific transcriptional variability that could be related to other biological features, whether or not these are involved in the pathogenesis of amebiasis.
Valle
P R
PR
Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brasil.
Souza
M B
MB
Pires
E M
EM
Silva
E F
EF
Gomes
M A
MA
D000818:Animals; D016172:DNA Fingerprinting / Q000379:methods*; D018076:DNA, Complementary; D016054:DNA, Protozoan / Q000032:analysis*; D004748:Entamoeba histolytica / Q000235:genetics* / Q000472:pathogenicity; D015870:Gene Expression; D016133:Polymerase Chain Reaction / Q000379:methods*; D016053:RNA, Protozoan / Q000032:analysis*; D019105:Random Amplified Polymorphic DNA Technique; D014774:Virulence / Q000235:genetics
D018076:DNA, Complementary; D016054:DNA, Protozoan; D016053:RNA, Protozoan