[Multiple types of cytokines secreted by a mouse fetal liver stromal cell line].
A murine fetal liver stromal cell line, called MFLC, has been established and maintained in DME supplemented with 10%NCS for 2 years. Without exogenous stimulation, the MFLC cells spontaneously secreted several types of cytokines, including IL-6, GM-CSF and chemotactic factor. Of which, IL-6 and chemotactic factor were abundant, GM-CSF at low level. Biological activities of IL-7 and IL-3 were not detected in the MFLC cell's supernatants. MFLC-SN induced colony formation of murine bone marrow cells in a dose dependent fashion in which mixed granulocyte/macrophage/megakaryocyte colonies(CFU-GMM) and granulocyte/macrophage colonies(CFU-GM) were dominant. MFLC-SN also sustained colony formation of bone marrow cells taken from 5-Fu injected mice, suggesting that there was a biological activity similar to SCF in MFLC-SN. The cytokines secreted by MFLC might play an important role in T cell early development in fetal liver. These results will be useful for us to analyse the mechanism of T cell development in fetal liver and to study the cytokine network regulation.
Liu
Q
Q
Department of Immunology, Beijing Medical University.
Liu
Z G
ZG
Chen
W F
WF
Zhang
P X
PX
Pang
X W
XW
Qian
X P
XP
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002460:Cell Line; D002630:Chemotactic Factors / Q000378:metabolism; D003114:Colony-Forming Units Assay; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D005333:Fetus; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D017154:Stromal Cells / Q000378:metabolism
D002630:Chemotactic Factors; D016207:Cytokines; D015850:Interleukin-6; D016178:Granulocyte-Macrophage Colony-Stimulating Factor