[Transacting factors binding to the positive control region (PCR) in the 5' flanking sequence of human beta-globin gene].
Using gel mobility shift assay, three protein factors (Pa, Pb, Pc) binding to the positive control region (PCR) in the 5' flanking sequence of human beta-globin gene were detected in the nuclear extracts from mouse fetal liver at d 18 or d 19 of gestation. Competitive experiment showed that Pb and Pc could bind to GATA-1 motif, therefore could be the members of GATA family. While Pa was a new and developmental stage specific trans-acting factor, we suggested that the factor Pa was related to the activation of beta-globin gene.
Chen
S Y
SY
Shanghai Institute of Cell Biology, Academia Sinica.
Xie
H Y
HY
Jiang
C
C
Qian
R L
RL
D000818:Animals; D005914:Globins / Q000235:genetics*; D051379:Mice; D009687:Nuclear Proteins / Q000737:chemistry; D015534:Trans-Activators / Q000302:isolation & purification* / Q000378:metabolism
D009687:Nuclear Proteins; D015534:Trans-Activators; D005914:Globins