Characterization of immune escape viruses from a macaque immunized with live-virus vaccine and challenged with pathogenic SHIVKU-1.
We characterized two immune escape viruses (SHIV(KU-1/105w52) and SHIV(KU-1/105w98)) from a macaque immunized with DeltavpuDeltanef SHIV-4 and challenged with pathogenic SHIV(KU-1). This macaque developed neutralizing antibodies as well as virus-specific CTLs against the challenge virus. However, the two new viruses could not be neutralized by anti-SHIV(KU-1)-specific neutralizing antibodies and were poorly recognized by challenge virus-specific CTLs. Sequence analysis of the gene encoding gp120 revealed several mutations in the protein that might have contributed to the development of the immune-escape viruses.
Stipp
H L
HL
Merrell Dow Laboratory of Viral Pathogenesis and Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Center, Kansas City, Kansas 66160, USA.
Kumar
A
A
Narayan
O
O
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D001483:Base Sequence; D004279:DNA, Viral; D015693:Gene Products, nef / Q000276:immunology; D015699:HIV Envelope Protein gp120 / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics* / Q000276:immunology; D054298:Human Immunodeficiency Virus Proteins; D006801:Humans; D008251:Macaca; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009500:Neutralization Tests; D015302:Simian Immunodeficiency Virus / Q000235:genetics / Q000276:immunology*; D014611:Vaccination; D019562:Viral Load; D054334:Viral Regulatory and Accessory Proteins / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*; D054311:nef Gene Products, Human Immunodeficiency Virus; D000086082:Viroporin Proteins
D000914:Antibodies, Viral; D004279:DNA, Viral; D015693:Gene Products, nef; D015699:HIV Envelope Protein gp120; D054298:Human Immunodeficiency Virus Proteins; D054334:Viral Regulatory and Accessory Proteins; D014765:Viral Vaccines; D054311:nef Gene Products, Human Immunodeficiency Virus; C514859:vpu protein, Human immunodeficiency virus 1; D000086082:Viroporin Proteins