Low leukocyte contamination without filtration by preparation of platelet concentrates in cylindrical bags with the buffy-coat method.
OBJECTIVE
The buffy-coat (BC) method for platelet concentrate (PC) preparation was modified in order to obtain leukodepleted PCs from single BCs without filtration.


METHODS
BCs were centrifuged in cylindrical BC bags and the optimal centrifugation conditions and optimal hematocrit were determined.


RESULTS
With optimal conditions, a tenfold lower leukocyte contamination was obtained compared with the conventionally shaped, wide BC bag (0.3 +/- 0.19 versus 3.0 +/- 1.71 x 10(6) leukocytes per unit; 85-ml BCs). The platelet yield obtained with the cylindrical bag did not differ significantly from the yield obtained with the conventional bag (56 +/- 16.4 versus 61 +/- 15 x 10(9) platelets per PC). Furthermore, when PCs were prepared from 100-ml BCs in cylindrical bags, a leukocyte contamination of 0.2 +/- 0.11 x 10(6) and a platelet content of 61 +/- 13.5 x 10(9) per PC were obtained.


CONCLUSIONS
The use of cylindrical BC bags reduced the leukocyte contamination in PCs to a level required for leuko-depletion without affecting platelet recovery.
Kostelijk
E H
EH
Department of Blood Transfusion Technology, Division CLB of Sanquin Blood Supply Foundation, Amsterdam, The Netherlands.
Loos
J A
JA
Veldman
H A
HA
de Korte
D
D
D002469:Cell Separation / Q000295:instrumentation; D002498:Centrifugation / Q000379:methods; D004864:Equipment and Supplies / Q000592:standards; D005374:Filtration; D006400:Hematocrit; D006801:Humans; D007958:Leukocyte Count; D007962:Leukocytes; D010976:Platelet Count; D010983:Plateletpheresis / Q000379:methods* / Q000592:standards