title: Progression of a loop-loop complex to a four-way junction is crucial for the activity of a regulatory antisense RNA.
abstract: The antisense RNA, CopA, regulates the replication frequency of plasmid R1 through inhibition of RepA translation by rapid and specific binding to its target RNA (CopT). The stable CopA-CopT complex is characterized by a four-way junction structure and a side-by-side alignment of two long intramolecular helices. The significance of this structure for binding in vitro and control in vivo was tested by mutations in both CopA and CopT. High rates of stable complex formation in vitro and efficient inhibition in vivo required initial loop-loop complexes to be rapidly converted to extended interactions. These interactions involve asymmetric helix progression and melting of the upper stems of both RNAs to promote the formation of two intermolecular helices. Data presented here delineate the boundaries of these helices and emphasize the need for unimpeded helix propagation. This process is directional, i.e. one of the two intermolecular helices (B) must form first to allow formation of the other (B'). A binding pathway, characterized by a hierarchy of intermediates leading to an irreversible and inhibitory RNA-RNA complex, is proposed.
authors.affiliation: UPR 9002 du CNRS, Institut de Biologie Moléculaire et Cellulaire, 15 rue R. Descartes, Strasbourg cedex, France.
authors.forename: F A
authors.initials: FA
authors.lastname: Kolb
authors.forename: H M
authors.initials: HM
authors.lastname: Engdahl
authors.forename: J G
authors.initials: JG
authors.lastname: Slagter-Jäger
authors.forename: B
authors.initials: B
authors.lastname: Ehresmann
authors.forename: C
authors.initials: C
authors.lastname: Ehresmann
authors.forename: E
authors.initials: E
authors.lastname: Westhof
authors.forename: E G
authors.initials: EG
authors.lastname: Wagner
authors.forename: P
authors.initials: P
authors.lastname: Romby
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D001667:Binding, Competitive; D017931:DNA Primers / Q000235:genetics; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005798:Genes, Bacterial; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D016372:RNA, Antisense / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; C071263:CopA protein, Bacteria; C073592:CopT protein, Plasmid R1; D017931:DNA Primers; D016372:RNA, Antisense; D012329:RNA, Bacterial