title: Signal peptidase and oligosaccharyltransferase interact in a sequential and dependent manner within the endoplasmic reticulum.
abstract: We demonstrate that the signal peptides of prepro-alpha-factor and preinvertase must be cleaved before Asn-X-Ser/Thr acceptor tripeptides located near the signal peptides of these precursors can be efficiently glycosylated within the endoplasmic reticulum of the yeast Saccharomyces cerevisiae. The data support a model whereby the interaction of a signal peptide with the membrane prevents an acceptor tripeptide juxtaposed to the signal peptide from accessing the oligosaccharyltransferase active site until the signal peptide is cleaved.
authors.affiliation: Department of Microbiology and Immunology, School of Medicine, Vanderbilt University, Nashville, Tennessee 37232-2363, USA.
authors.forename: X
authors.initials: X
authors.lastname: Chen
authors.forename: C
authors.initials: C
authors.lastname: VanValkenburgh
authors.forename: H
authors.initials: H
authors.lastname: Liang
authors.forename: H
authors.initials: H
authors.lastname: Fang
authors.forename: N
authors.initials: N
authors.lastname: Green
mesh_terms: D000595:Amino Acid Sequence; D004721:Endoplasmic Reticulum / Q000201:enzymology*; D004792:Enzyme Precursors / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism; D006031:Glycosylation; D006602:Hexosyltransferases; D008565:Membrane Proteins; D008954:Models, Biological; D008969:Molecular Sequence Data; D009154:Mutation; D010447:Peptide Hydrolases; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D012697:Serine Endopeptidases / Q000378:metabolism*; D014166:Transferases / Q000378:metabolism*; D043324:beta-Fructofuranosidase
chemical_list: D004792:Enzyme Precursors; D005656:Fungal Proteins; C048632:MF(ALPHA)1 protein, S cerevisiae; D008565:Membrane Proteins; D011498:Protein Precursors; D029701:Saccharomyces cerevisiae Proteins; D014166:Transferases; D006602:Hexosyltransferases; C025620:dolichyl-diphosphooligosaccharide - protein glycotransferase; D006026:Glycoside Hydrolases; C428436:preinvertase; D043324:beta-Fructofuranosidase; D010447:Peptide Hydrolases; C061149:SEC11 protein, S cerevisiae; D012697:Serine Endopeptidases; C026515:type I signal peptidase