Some in vitro invasion inhibition of red cells by in vivo nonprotective anti-LDH antibodies of Plasmodium berghei.
In Plasmodium berghei, sephadex G-200 purified lactate dehydrogenase (LDH) fraction immunized mice did not exhibit protection when challenged with 1 x 10(6) P. berghei-parasitized erythrocytes. However, LDH immunized mice seroconverted and showed an antibody titre of 1:2048 by indirect haemagglutination (IHA) and 1:160 by indirect fluorescent antibody (IFA) assays. Fluorescence was distributed evenly on P. berghei-parasitized red cells showing no specific location of parasite LDH. Anti-LDH antibodies supplemented in 19 h in vitro culture of P. berghei exhibited 9.2% invasion inhibition into the fresh red cells.
Kumar
S
S
Department of Veterinary Microbiology, Punjab Agricultural University, Ludhiana, India.
D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000276:immunology* / Q000469:parasitology*; D019084:Fluorescent Antibody Technique, Indirect; D006386:Hemagglutination Tests; D007114:Immunization / Q000662:veterinary; D007770:L-Lactate Dehydrogenase / Q000276:immunology*; D008288:Malaria / Q000517:prevention & control*; D051379:Mice; D010962:Plasmodium berghei / Q000276:immunology*
D000913:Antibodies, Protozoan; D007770:L-Lactate Dehydrogenase