Anti-Inflammatory properties of a lipid fraction obtained from Sideritis javalambrensis.
A lipid fraction obtained by activity-guided fractionation from the hexane extract of Sideritis javalambrensis was evaluated for anti-inflammatory activity. This fraction significantly inhibited paw oedema induced by carrageenan as well as ear oedema induced by 12-O-tetradecanoylphorbol 13-acetate (TPA) in mice after oral or topical administration, respectively. Quantitation of the specific marker myeloperoxidase (MPO) demonstrated that its topical anti-inflammatory activity was associated with reduction in cell infiltration into inflamed tissues. The lipid fraction significantly decreased leukocyte granular enzyme release (beta-glucuronidase), but failed to inhibit superoxide generation. Histamine release from mast cells was also suppressed in a concentration-dependent manner. In addition, non-toxic concentrations of this fraction reduced nitric oxide (NO) generation in lipopolysaccharide (LPS)-treated J774 macrophages. Taken together, these results suggest that the lipid fraction exerts in vivo anti-inflammatory activity with the partial contribution of inhibitory actions on some inflammatory responses.
Godo
A
A
Departamento de Farmacología, Facultad de Farmacia, Universidad Complutense de Madrid, Spain.
de Heras
B L
BL
Vivas
J M
JM
Villar
A
A
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000494:pharmacology*; D002351:Carrageenan; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002855:Chromatography, Thin Layer; D004487:Edema / Q000139:chemically induced / Q000517:prevention & control; D005260:Female; D005966:Glucuronidase / Q000378:metabolism; D006636:Histamine Release; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D019686:Lamiaceae / Q000737:chemistry*; D007962:Leukocytes / Q000187:drug effects; D008055:Lipids / Q000302:isolation & purification / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D013030:Spain; D013055:Spectrophotometry, Infrared; D013481:Superoxides / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate
D000894:Anti-Inflammatory Agents, Non-Steroidal; D008055:Lipids; D013481:Superoxides; D009569:Nitric Oxide; D002351:Carrageenan; D007770:L-Lactate Dehydrogenase; D005966:Glucuronidase; D013755:Tetradecanoylphorbol Acetate