VirB/D4-dependent protein translocation from Agrobacterium into plant cells.
The Agrobacterium VirB/D4 transport system mediates the transfer of a nucleoprotein T complex into plant cells, leading to crown gall disease. In addition, several Virulence proteins must somehow be transported to fulfill a function in planta. Here, we used fusions between Cre recombinase and VirE2 or VirF to directly demonstrate protein translocation into plant cells. Transport of the proteins was monitored by a Cre-mediated in planta recombination event resulting in a selectable phenotype and depended on the VirB/D4 transport system but did not require transferred DNA.
Vergunst
A C
AC
Institute of Molecular Plant Sciences, Leiden University, Clusius Laboratory, Wassenaarseweg 64, 2333 AL, Leiden, Netherlands.
Schrammeijer
B
B
den Dulk-Ras
A
A
de Vlaam
C M
CM
Regensburg-Tuïnk
T J
TJ
Hooykaas
P J
PJ
D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D001426:Bacterial Proteins / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004351:Drug Resistance; D019426:Integrases / Q000235:genetics / Q000378:metabolism*; D007473:Ion Channels; D007612:Kanamycin / Q000494:pharmacology; D018517:Plant Roots / Q000378:metabolism; D030821:Plants, Genetically Modified; D010957:Plasmids; D016133:Polymerase Chain Reaction; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014764:Viral Proteins; D014774:Virulence; D037521:Virulence Factors
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D007473:Ion Channels; D011993:Recombinant Fusion Proteins; C036483:T-DNA; D014764:Viral Proteins; D037521:Virulence Factors; C051583:virE2 protein, Agrobacterium; D007612:Kanamycin; C045073:Cre recombinase; D019426:Integrases