title: Matrix metalloproteinases correlate with alveolar-capillary permeability alteration in lung ischemia-reperfusion injury.
abstract: BACKGROUND
  Matrix metalloproteinases (MMPs) are able to degrade the endothelial basal lamina and increase vascular permeability.
  
  
  METHODS
  In a porcine model of isolated-reperfused lung, we studied the alveolar-capillary permeability and the zymographic expression of MMP-9 and MMP-2 in the bronchoalveolar lavage fluid of lungs submitted ex vivo to ischemia in three preservation solutions [modified Euro-Collins (EC), low-potassium-dextran, modified-blood]. Twenty-two pigs were randomly divided into three groups according to the preservation solution used. One lung of each pig was rapidly reperfused and analyzed (control lung) although the other lung was reperfused and analyzed after 8 hr of ischemia (ischemic lung).
  
  
  RESULTS
  Alveolar-capillary permeability, evaluated by the transferrin leak index, was increased after 8 hr of ischemia compared with controls in the three groups, but was significantly higher in the modified EC group. In the EC group, after 8 hr of ischemia, both proMMP-9 and MMP-9 increased significantly (8.8- and 22-fold, respectively) compared with controls and this increase correlated with the transferrin leak index. Neither proMMP-9 nor MMP-9 increased with the other two preservation solutions. The MMP-2 increase after ischemia was smaller and was also restricted to the EC group.
  
  
  CONCLUSIONS
  MMP expression is enhanced during lung ischemia-reperfusion, especially in the presence of EC and this phenomenon correlates with the alteration of alveolar-capillary permeability.
authors.affiliation: Department of Internal Medicine, Geneva University Hospital, Switzerland.
authors.forename: P M
authors.initials: PM
authors.lastname: Soccal
authors.forename: Y
authors.initials: Y
authors.lastname: Gasche
authors.forename: J C
authors.initials: JC
authors.lastname: Pache
authors.forename: O
authors.initials: O
authors.lastname: Schneuwly
authors.forename: D O
authors.initials: DO
authors.lastname: Slosman
authors.forename: D R
authors.initials: DR
authors.lastname: Morel
authors.forename: A
authors.initials: A
authors.lastname: Spiliopoulos
authors.forename: P M
authors.initials: PM
authors.lastname: Suter
authors.forename: L P
authors.initials: LP
authors.lastname: Nicod
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D002199:Capillary Permeability / Q000187:drug effects*; D002452:Cell Count; D007150:Immunohistochemistry; D007205:Indium Radioisotopes; D008168:Lung / Q000098:blood supply*; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis*; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis*; D015427:Reperfusion Injury / Q000201:enzymology*; D013552:Swine; D014018:Tissue Distribution; D014168:Transferrin / Q000378:metabolism
chemical_list: D007205:Indium Radioisotopes; D014168:Transferrin; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9