title: Crystallization and preliminary X-ray studies of oligandrin, a sterol-carrier elicitor from Pythium oligandrum.
abstract: Oligandrin is a 10 kDa acidic protein produced by the fungus micromycete Pythium oligandrum and is a member of the alpha-elicitin group, with sterol- and lipid-carrier properties. Oligandrin has been crystallized at 290 K using PEG 4000 as a precipitant. A cholesterol complex was obtained under the same conditions. The space group of the crystals at low temperature (100 K) is C222, with unit-cell parameters a = 94.0, b = 171.1, c = 55.3 A. Four molecules are present in the asymmetric unit. Data from the free and cholesterol-complexed forms were recorded at synchrotron sources to resolutions of 2.4 (uncomplexed) and 1.9 A (complexed), respectively.
authors.affiliation: LCRB - Laboratoire de Cristallographie et RMN Biologiques (EP-2075, CNRS), Faculté de Pharmacie, 4 Avenue de l'Observatoire, 75270 Paris CEDEX 06, France.
authors.forename: M B
authors.initials: MB
authors.lastname: Lascombe
authors.forename: M L
authors.initials: ML
authors.lastname: Milat
authors.forename: J P
authors.initials: JP
authors.lastname: Blein
authors.forename: F
authors.initials: F
authors.lastname: Panabières
authors.forename: M
authors.initials: M
authors.lastname: Ponchet
authors.forename: T
authors.initials: T
authors.lastname: Prangé
mesh_terms: D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism; D002784:Cholesterol / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification; D036341:Intercellular Signaling Peptides and Proteins; D011487:Protein Conformation; D011775:Pythium / Q000737:chemistry*; D013261:Sterols / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D005656:Fungal Proteins; D036341:Intercellular Signaling Peptides and Proteins; C415980:Oligandrin protein, Pythium oligandrum; D013261:Sterols; D002784:Cholesterol