title: Helicobacter pylori-related and -non-related gastric cancers do not differ with respect to chromosomal aberrations.
abstract: Gastric carcinogenesis is strongly associated with Helicobacter pylori infection, but the underlying genetic mechanisms are largely unknown. The aim of this study was to correlate chromosomal aberrations in gastric cancer to H. pylori status and its different strains, as well as to histological type and other clinico-pathological variables. DNA from 46 gastric cancers (male/female 35/11, age 27-85 years) was extracted from formaldehyde-fixed, paraffin-embedded material and tested for chromosomal gains and losses by comparative genomic hybridization (CGH). Chromosomal aberrations with frequencies of 20% or higher were considered to be non-random changes associated with gastric cancer. The mean number of chromosomal events per tumour was 9.7 (range 0-27), with a mean of 3.2 gains (range 0-16) and 6.5 losses (range 0-15). Gains were most frequently found at chromosomes 8q and 13q (24% and 26%, respectively). Losses were predominantly found on chromosome arms 2q, 9p, 12q, 14q, 15q, 16p, 16q, 17p, 17q, 19p, 19q, and 22q (22%, 30%, 43%, 22%, 33%, 50%, 28%, 50%, 39%, 33%, 39%, and 37%, respectively). Common regions of overlap narrowed down to 2q11-14, 8q23, 9p21, 12q24, 13q21-22, 14q24 and 15q11-15. The mean number of gains was higher in tumours with metastases than in localized tumours (4.1 vs. 1.9, p=0.04). Tumours with a loss at 17p showed a higher number of losses than tumours without a 17p loss (9. 5 vs. 4.7 on average, p<0.001). Neither H. pylori status (+, n=25; -, n=21) nor H. pylori strain was correlated to the total number of events or to any specific chromosomal aberration, nor were there differences between intestinal (n=30) and diffuse (n=15) cancers or any other clinico-pathological variable tested. In conclusion, a complex of chromosomal aberrations is involved in gastric cancer, but their pattern does not depend on H. pylori status or strain, nor on the histological type of the tumour. The exact biological meaning of these aberrations in carcinogenesis needs further clarification.
authors.affiliation: Department of Gastroenterology, Free University Hospital, Amsterdam, The Netherlands.
authors.forename: N C
authors.initials: NC
authors.lastname: van Grieken
authors.forename: M M
authors.initials: MM
authors.lastname: Weiss
authors.forename: G A
authors.initials: GA
authors.lastname: Meijer
authors.forename: M A
authors.initials: MA
authors.lastname: Hermsen
authors.forename: G H
authors.initials: GH
authors.lastname: Scholte
authors.forename: J
authors.initials: J
authors.lastname: Lindeman
authors.forename: M E
authors.initials: ME
authors.lastname: Craanen
authors.forename: E
authors.initials: E
authors.lastname: Bloemena
authors.forename: S G
authors.initials: SG
authors.lastname: Meuwissen
authors.forename: J P
authors.initials: JP
authors.lastname: Baak
authors.forename: E J
authors.initials: EJ
authors.lastname: Kuipers
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002869:Chromosome Aberrations; D004247:DNA / Q000032:analysis; D005260:Female; D005434:Flow Cytometry; D016481:Helicobacter Infections / Q000150:complications*; D016480:Helicobacter pylori; D006801:Humans; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D013274:Stomach Neoplasms / Q000209:etiology*
chemical_list: D004247:DNA