Equine infectious anaemia virus proteins with epitopes most frequently recognized by cytotoxic T lymphocytes from infected horses.
Efficacious lentiviral vaccines designed to induce cytotoxic T lymphocytes (CTL) in outbred populations with a diverse repertoire of MHC class I molecules should contain or express multiple viral proteins. To determine the equine infectious anaemia virus (EIAV) proteins with epitopes most frequently recognized by CTL from seven horses infected for 0.5 to 7 years, retroviral vector-transduced target cells expressing viral proteins were used in CTL assays. Gag p15 was recognized by CTL from 100% of these infected horses. p26 was recognized by CTL from 86%, SU and the middle third of Pol protein were each recognized by 43%, TM by 29%, and S2 by 14%. Based on these results, it is likely that a construct expressing the 359 amino acids constituting p15 and p26 would contain epitopes capable of stimulating CTL in most horses.
McGuire
Travis C
TC
Department of Veterinary Microbiology and Pathology1 and Department of Animal Sciences2, Washington State University, Pullman, WA 99164-7040, USA.
Leib
Steven R
SR
Department of Veterinary Microbiology and Pathology1 and Department of Animal Sciences2, Washington State University, Pullman, WA 99164-7040, USA.
Lonning
Scott M
SM
Department of Veterinary Microbiology and Pathology1 and Department of Animal Sciences2, Washington State University, Pullman, WA 99164-7040, USA.
Zhang
Wei
W
Department of Veterinary Microbiology and Pathology1 and Department of Animal Sciences2, Washington State University, Pullman, WA 99164-7040, USA.
Byrne
Katherine M
KM
Department of Veterinary Microbiology and Pathology1 and Department of Animal Sciences2, Washington State University, Pullman, WA 99164-7040, USA.
Mealey
Robert H
RH
Department of Veterinary Microbiology and Pathology1 and Department of Animal Sciences2, Washington State University, Pullman, WA 99164-7040, USA.
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D000939:Epitopes / Q000276:immunology*; D004859:Equine Infectious Anemia / Q000276:immunology*; D006736:Horses / Q000276:immunology / Q000821:virology; D004860:Infectious Anemia Virus, Equine / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000956:Antigens, Viral; D000939:Epitopes