Algal-lytic activities encoded by Chlorella virus CVK2.
Using a halo assay with E. coli lysates expressing Chlorella virus CVK2 genes on a cosmid contig, two different algal-lytic activities against Chlorella strain NC64A cells were found to be encoded on the CVK2 genome. The gene for vAL-1, one of the two activities, encoded a 349-aa ORF, which was homologous to PBCV-1 A215L and CVN1 CL-2. The vAL-1 gene was expressed at relatively early stages of the virus life cycle; transcripts and translation products appeared at 60 and 90 min postinfection, respectively. The vAL-1 protein was not incorporated into the viral particles but remained in the cell lysate, suggesting a role in the digestion of the cell wall before viral release at the final stage of infection. Cell wall materials isolated from Chlorella strain NC64A cells were digested by vAL-1 and degradation products were detected on TLC. In addition to Chlorella strain NC64A, vAL-1 lysed cells of four C. vulgaris strains as well as Chlorella sp. SAG-241-80.
Sugimoto
I
I
Department of Molecular Biotechnology, Graduate School of ADSM, Hiroshima University, 1-4-1 Kagamiyama, Hiroshima, Higashi, 739-8527, Japan.
Hiramatsu
S
S
Murakami
D
D
Fujie
M
M
Usami
S
S
Yamada
T
T
D000595:Amino Acid Sequence; D002708:Chlorella / Q000821:virology*; D003001:Cloning, Molecular; D011088:DNA Ligases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli; D005814:Genes, Viral; D008018:Life Cycle Stages; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017862:Phycodnaviridae / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics*
D014764:Viral Proteins; C110456:Chlorella virus DNA ligase; D011088:DNA Ligases