title: Plasminogen did not affect lung function in surfactant-treated preterm lambs.
abstract: Preterm infants with respiratory distress syndrome develop fibrin-rich hyaline membranes within the alveoli and have depressed fibrinolytic activity, which is thought to be due to a relative deficiency of plasminogen. Local fibrin deposition inhibits surfactant function and amplifies inflammation. We hypothesized that plasminogen administration to surfactant-treated preterm lambs would prevent fibrin-rich hyaline membrane formation, resulting in the amelioration of lung pathology and improved lung function. We randomly treated preterm lambs (gestational age 127-129 days) with either 16 mg of lysine-plasminogen (n = 10) or saline (n = 10), and ventilated them for 5 h. There were no significant differences in physiologic measurements of lung function (ventilation efficiency index, oxygenation index, dynamic compliance, quasi-static pressure volume curve), measures of lung injury (alveolar wash protein content and (125)I-albumin recovery) or surfactant pool size. The degree and extent of bronchiolar erosion and hyaline membrane formation were similar in the two groups. Plasminogen administration did not improve lung function or prevent hyaline membrane formation in surfactant-treated lambs.
authors.affiliation: Children's Hospital Medical Center, Cincinnati, Ohio 45229-3039, USA.
authors.forename: V
authors.initials: V
authors.lastname: Bhat
authors.forename: A H
authors.initials: AH
authors.lastname: Jobe
authors.forename: S
authors.initials: S
authors.lastname: Wert
authors.forename: G J
authors.initials: GJ
authors.lastname: Jesmok
authors.forename: M
authors.initials: M
authors.lastname: Ikegami
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D001980:Bronchi / Q000187:drug effects / Q000473:pathology; D005865:Gestational Age; D006818:Hyalin / Q000502:physiology; D008168:Lung / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology; D010446:Peptide Fragments / Q000627:therapeutic use*; D010958:Plasminogen / Q000627:therapeutic use*; D011663:Pulmonary Surfactants / Q000378:metabolism / Q000494:pharmacology*; D012756:Sheep; D013500:Surface Tension
chemical_list: D010446:Peptide Fragments; D011663:Pulmonary Surfactants; C018998:lysyl-plasminogen; D010958:Plasminogen