title: Salmonella flagellin fused with a linear epitope of colonization factor antigen I (CFA/I) can prime antibody responses against homologous and heterologous fimbriae of enterotoxigenic Escherichia coli.
abstract: In this work, a 15-amino-acid-long peptide derived from the enterotoxigenic Escherichia coli CFA/I fimbria (11VDPVIDLLQADGNAL25) was genetically fused to the Salmonella flagellin and used to prime and boost serum antibody responses (IgG) against homologous (CFA/I) and heterologous (CS1) colonization factors (CFs) in BALB/c mice. Antibodies raised against the hybrid flagellin (Fla II) cross-reacted with CFA/I, CS1, CS2, and PCFO166 but not with CS4. Parenteral administration of Fla II primed antibody responses against both CFA/I and CS1 but boosted IgG responses only against CFA/I. These findings confirm that linear epitopes derived from the CFA/I fimbria can prime antibody responses against homologous and heterologous CFs and indicate that Salmonella flagellin represents a potential carrier for the development of broad-range peptide-based anti-colonization ETEC vaccines.
authors.affiliation: Disciplina de Microbiologia e Imunologia, Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Brazil. luna@uerj.br
authors.forename: M
authors.initials: M
authors.lastname: das Graças Luna
authors.forename: F F
authors.initials: FF
authors.lastname: Sardella
authors.forename: L C
authors.initials: LC
authors.lastname: Ferreira
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology*; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology; D004926:Escherichia coli / Q000276:immunology*; D005260:Female; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000276:immunology*; D005408:Flagellin / Q000276:immunology*; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000276:immunology; D012475:Salmonella / Q000276:immunology*; D013045:Species Specificity
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D000939:Epitopes; D011993:Recombinant Fusion Proteins; C022924:colonization factor antigens; D005408:Flagellin; D036781:Fimbriae Proteins