A tetanus toxin sensitive protein other than VAMP 2 is required for exocytosis in the pancreatic acinar cell.
The neurotoxin sensitivity of regulated exocytosis in the pancreatic acinar cell was investigated using streptolysin-O permeabilized pancreatic acini. Treatment of permeabilized acini with botulinum toxin B (BoNT/B) or botulinum toxin D (BoNT/D) had no detectable effect on Ca(2+)-dependent amylase secretion but did result in the complete cleavage of VAMP 2. In comparison, tetanus toxin (TeTx) treatment both significantly inhibited Ca(2+)-dependent amylase secretion and cleaved VAMP 2. These results indicate that regulated exocytosis in the pancreatic acinar cell requires a tetanus toxin sensitive protein(s) other than VAMP 2.
Padfield
P J
PJ
Department of Pathology, Saint Louis University Medical Centre, South Grand Boulevard, St. Louis, MO 63104, USA. ppadfiel@fs1.ho.man.ac.uk
D000681:Amylases / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D005089:Exocytosis / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D008565:Membrane Proteins / Q000378:metabolism / Q000502:physiology*; D010179:Pancreas / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D050683:R-SNARE Proteins; D051381:Rats; D050600:SNARE Proteins; D013744:Tetanus Toxin / Q000494:pharmacology*; D033921:Vesicular Transport Proteins
D008565:Membrane Proteins; D050683:R-SNARE Proteins; D050600:SNARE Proteins; D013744:Tetanus Toxin; D033921:Vesicular Transport Proteins; D000681:Amylases; D002118:Calcium