title: Gene transfer of recombinant endothelial nitric oxide synthase to liver in vivo and in vitro.
abstract: Endothelial nitric oxide synthase (eNOS)-derived nitric oxide (NO) contributes to hepatic vascular homeostasis. The aim of this study was to examine whether delivery of an adenoviral vector encoding eNOS gene to liver affects vasomotor function in vivo and the mechanism of NO production in vitro. Rats were administered adenoviruses encoding beta-galactosidase (AdCMVLacZ) or eNOS (AdCMVeNOS) via tail vein injection and studied 1 wk later. In animals transduced with AdCMVLacZ, beta-galactosidase activity was increased in the liver, most prominently in hepatocytes. In AdCMVeNOS-transduced animals, eNOS protein levels and catalytic activity were significantly increased. Overexpression of eNOS diminished baseline perfusion pressure and constriction in response to the alpha(1)-agonist methoxamine in the perfused liver. Transduction of cultured hepatocytes with AdCMVeNOS resulted in the targeting of recombinant eNOS to a perinuclear distribution and binding with the NOS-activating protein heat shock protein 90. These events were associated with increased ionomycin-stimulated NO release. In summary, this is the first study to demonstrate successful delivery of the recombinant eNOS gene to liver in vivo and in vitro with ensuing NO production.
authors.affiliation: Gastrointestinal Research Unit and Anesthesia Research Unit, Mayo Clinic, Rochester, Minnesota 55905, USA. shah.vijay@mayo.edu
authors.forename: V
authors.initials: V
authors.lastname: Shah
authors.forename: A F
authors.initials: AF
authors.lastname: Chen
authors.forename: S
authors.initials: S
authors.lastname: Cao
authors.forename: H
authors.initials: H
authors.lastname: Hendrickson
authors.forename: D
authors.initials: D
authors.lastname: Weiler
authors.forename: L
authors.initials: L
authors.lastname: Smith
authors.forename: J
authors.initials: J
authors.lastname: Yao
authors.forename: Z S
authors.initials: ZS
authors.lastname: Katusic
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D000001:Calcimycin; D018014:Gene Transfer Techniques; D022781:Hepatocytes / Q000166:cytology / Q000201:enzymology*; D066298:In Vitro Techniques; D007476:Ionophores; D007763:Lac Operon; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000235:genetics*; D052250:Nitric Oxide Synthase Type III; D012323:RNA Processing, Post-Transcriptional / Q000502:physiology; D051381:Rats; D011916:Rats, Inbred F344; D011994:Recombinant Proteins / Q000235:genetics; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D007476:Ionophores; D011994:Recombinant Proteins; D009569:Nitric Oxide; D000001:Calcimycin; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat; D001616:beta-Galactosidase