Fucoidan is the active component of fucus vesiculosus that promotes contraction of fibroblast-populated collagen gels.
The fibroblast-populated collagen gel culture method has been evaluated as a dermal model of wound contraction and granulation in tissues during the wound healing process and as an in vitro model of dermal tissue. We previously reported that an extract of Fucus vesiculosus promoted fibroblast-populated collagen gel contraction and that the promotion of the gel contraction was due to the increased expression of integrin alpha2beta1 on the surface of the fibroblasts. In this study, we investigated the active component of the extract of this alga using extraction and fractionation techniques. Water extraction of the alga was followed by precipitation with excess ethanol and then gel filtration with the boundary molecular weight of 30,000. The high molecular weight fraction obtained from gel filtration was fractionated by ion exchange chromatography on diethylaminoethyl cellulose column to give active fractions that have more polar properties. These polar, high molecular weight fractions which contained molecules with fucose and sulfate groups showed significant gel contraction-promoting activity and integrin expression-enhancing activity, and were estimated to be the sulfated-polysaccharide fucoidan. Commercially available fucoidan showed similar activities to the above-described fraction of this alga. Although it remains necessary to precisely identify the specific active component, the above results indicate that fucoidan is the active component which promotes collagen gel contraction, and also indicate the possibility that it dose so by enhancing the integrin alpha2beta1 expression.
Fujimura
T
T
Biological Science Laboratories, Kao Corporation, Haga, Tochigi, Japan. 301620@kastanet.kao.co.jp
Shibuya
Y
Y
Moriwaki
S
S
Tsukahara
K
K
Kitahara
T
T
Sano
T
T
Nishizawa
Y
Y
Takema
Y
Y
D000925:Anticoagulants / Q000494:pharmacology*; D002452:Cell Count; D002478:Cells, Cultured; D002850:Chromatography, Gel; D003094:Collagen; D005347:Fibroblasts / Q000187:drug effects; D005434:Flow Cytometry; D005782:Gels; D008970:Molecular Weight; D000459:Phaeophyceae / Q000737:chemistry*; D011134:Polysaccharides / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects*
D000925:Anticoagulants; D005782:Gels; D011134:Polysaccharides; D003094:Collagen; C007789:fucoidan