Effects of galanin on wide-dynamic range neuron activity in the spinal dorsal horn of rats with sciatic nerve ligation.
Galanin is a 29-amino acid peptide with a suggested role in nociception. The effect of galanin on wide-dynamic range neuron discharge frequency in rats with nerve ligation, used as a model of neurogenic pain, was investigated by extracellular recording methods. Seven to 14 days after sciatic nerve ligation, 0.1, 0.5 or 1 nmol of galanin was administered directly on the dorsal surface of the L3-L5 spinal cord of rats with sciatic nerve ligation. It was found that galanin inhibited the activity of wide-dynamic range neurons dose-dependently, an effect was more pronounced in sciatic nerve ligated rats than intact rats. Furthermore, when 1 nmol of galantide, the galanin antagonist, was administered on the dorsal surface of the L3-L5 spinal cord, the wide-dynamic range neuron discharge frequency increased significantly. The results suggest that galanin plays an important role in the modulation of presumed nociception in mononeuropathy.
Xu
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S
Department of Physiology, College of Life Sciences, and National Laboratory of Biomembranes and Membrane Biotechnology, Peking University, 100871, Beijing, PR China.
Zhang
Y
Y
Lundeberg
T
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Yu
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L
D000818:Animals; D004558:Electric Stimulation; D019004:Galanin / Q000031:analogs & derivatives / Q000494:pharmacology*; D008297:Male; D020671:Posterior Horn Cells / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D044088:Receptors, Galanin; D018013:Receptors, Neuropeptide / Q000502:physiology; D012584:Sciatic Nerve / Q000502:physiology*; D013373:Substance P / Q000031:analogs & derivatives / Q000494:pharmacology; D013997:Time Factors
D044088:Receptors, Galanin; D018013:Receptors, Neuropeptide; C074975:galantide; D013373:Substance P; D019004:Galanin