Vancomycin-resistant Enterococcus faecium infection in three children given allogeneic hematopoietic stem cell transplantation: clinical and microbiologic features.
OBJECTIVE
The emergence of vancomycin-resistant enterococci (VRE) as nosocomial pathogens is a major problem in the US; in Europe, VRE nosocomial infections are uncommon and only rarely have been reported in Pediatric or Neonatal Units. The aim of this study is to report on the clinical and microbiological features of VRE infections in 3 children given hematopoietic stem cell transplantation (HSCT).


METHODS
Five episodes of vancomycin-resistant Enterococcus faecium (VREF) infection were diagnosed in 3 children given an allogeneic HSCT. Molecular methods, such as random amplification of polymorphic DNA (RAPD) fingerprinting and automated ribotyping, were used in order to define the circulation of strains.


RESULTS
All the isolates were resistant to all commercially available agents and showed the VanA genotypic profile. All children were successfully treated with the combination of quinupristin/dalfopristin (QD) plus teicoplanin (TEC), although treatment was not sufficient to eradicate the micro-organism promptly from the gastrointestinal tract. All our children are still alive. After the first isolation of VRE, a surveillance protocol was started and we documented that the rate of colonization in children and their mothers was less than 1.5%. The RAPD method demonstrated the possible nosocomial transmission of one strain.


CONCLUSIONS
Our experience demonstrates that VRE infection is a life-threatening complication in children given HSCT. Prompt diagnosis of this infection and its treatment with the combination of QD and TEC can successfully manage this severe infection in profoundly immunocompromised patients.
Carretto
E
E
Laboratorio di Batteriologia e Micologia, Laboratori Sperimentali di Ricerca, Area Infettivologica, IRCCS Policlinico San Matteo, viale Taramelli 5, 27100 Pavia, Italy. e.carretto@smatteo.pv.it
Barbarini
D
D
Locatelli
F
F
Giraldi
E
E
Pellegrini
N
N
Perversi
L
L
Grossi
P
P
Marone
P
P
Bonetti
F
F
D000900:Anti-Bacterial Agents / Q000008:administration & dosage; D002675:Child, Preschool; D004359:Drug Therapy, Combination; D016984:Enterococcus faecium / Q000187:drug effects*; D005260:Female; D016908:Gram-Positive Bacterial Infections / Q000188:drug therapy / Q000209:etiology; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D006801:Humans; D007223:Infant; D008297:Male; D010455:Peptides; D014184:Transplantation, Homologous / Q000009:adverse effects; D020713:Vancomycin Resistance
D000900:Anti-Bacterial Agents; D010455:Peptides