title: Detection of Chlamydia pneumoniae DNA in CD3+ lymphocytes from healthy blood donors and patients with coronary artery disease.
abstract: BACKGROUND
  Chlamydia pneumoniae is an intracellular bacterium responsible for respiratory tract infections. Recent studies have implicated this organism in the pathogenesis of atherosclerosis.
  
  
  RESULTS
  To address how the organism is transported from lungs to cardiac vessels, we characterized the cell population within peripheral blood mononuclear cells (PBMCs) that harbor C pneumoniae DNA. Adherent and nonadherent PBMCs from 28 patients with coronary artery disease (CAD) and 19 healthy blood donors were evaluated for the presence of C pneumoniae DNA by touchdown nested polymerase chain reaction (nPCR). Of the 28 patients, 10 (36%) had detectable PCR product in their nonadherent and 3 (10%) in their adherent PBMC population. C pneumoniae-specific PCR results were positive for 5 of 19 (26%) healthy blood donors. PCR positivity was detected only in the nonadherent cell population among this group of individuals. Fractionation of nonadherent PBMCs identified C pneumoniae-specific PCR signal among the CD3+ T-cell population exclusively. Of the 18 PCR-positive subjects (13 patients and 5 healthy control subjects), 67% (8 patients and 4 healthy blood donors) tested positive for C pneumoniae-specific IgG serology. Interestingly, 2 patients became PCR negative on a repeated blood draw 5 months after initial detection of C pneumoniae DNA despite retaining C pneumoniae-specific antibodies.
  
  
  CONCLUSIONS
  Our results demonstrate marginally significant prevalence of C pneumoniae DNA in patients with CAD compared with healthy subjects (P=0.082). In contrast, the prevalence of IgG seropositivity among the 2 groups did not reach statistical significance (P=0.306). We also provide unequivocal evidence for the presence of C pneumoniae DNA predominantly among the circulating CD3+ T-cell population.
authors.affiliation: Section of Infectious Diseases, Department of Pediatrics, Division of Cardiovascular Medicine, University of California, Davis, USA. rkkaul@ucdavis.edu
authors.forename: R
authors.initials: R
authors.lastname: Kaul
authors.forename: J
authors.initials: J
authors.lastname: Uphoff
authors.forename: J
authors.initials: J
authors.lastname: Wiedeman
authors.forename: S
authors.initials: S
authors.lastname: Yadlapalli
authors.forename: W M
authors.initials: WM
authors.lastname: Wenman
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001782:Blood Donors; D017252:CD3 Complex / Q000737:chemistry* / Q000276:immunology; D016993:Chlamydophila pneumoniae / Q000737:chemistry*; D003327:Coronary Disease / Q000097:blood* / Q000382:microbiology; D004269:DNA, Bacterial / Q000097:blood*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000706:statistics & numerical data; D013601:T-Lymphocytes / Q000737:chemistry* / Q000276:immunology
chemical_list: D017252:CD3 Complex; D004269:DNA, Bacterial; D007074:Immunoglobulin G