Eosinophil major basic protein-1 does not contribute to allergen-induced airway pathologies in mouse models of asthma.
The relationship between eosinophils and the development of Ag-induced pulmonary pathologies, including airway hyper-responsiveness, was investigated using mice deficient for the secondary granule component, major basic protein-1 (mMBP-1). The loss of mMBP-1 had no effect on OVA-induced airway histopathologies or inflammatory cell recruitment. Lung function measurements of knockout mice demonstrated a generalized hyporeactivity to methacholine-induced airflow changes (relative to wild type); however, this baseline phenotype was observable only with methacholine; no relative airflow changes were observed in response to another nonspecific stimulus (serotonin). Moreover, OVA sensitization/aerosol challenge of wild-type and mMBP-1(-/-) mice resulted in identical dose-response changes to either methacholine or serotonin. Thus, the airway hyper-responsiveness in murine models of asthma occurs in the absence of mMBP-1.
Denzler
K L
KL
Department of Biochemistry and Molecular Biology, Mayo Clinic Scottsdale, Samuel C. Johnson Medical Research Building, Scottsdale, AZ 85259, USA.
Farmer
S C
SC
Crosby
J R
JR
Borchers
M
M
Cieslewicz
G
G
Larson
K A
KA
Cormier-Regard
S
S
Lee
N A
NA
Lee
J J
JJ
D000485:Allergens / Q000008:administration & dosage / Q000276:immunology*; D000818:Animals; D000947:Antigens, Helminth / Q000008:administration & dosage; D001249:Asthma / Q000235:genetics / Q000276:immunology* / Q000473:pathology*; D001798:Blood Proteins / Q000096:biosynthesis / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D016535:Bronchial Hyperreactivity / Q000235:genetics / Q000276:immunology; D002465:Cell Movement / Q000235:genetics / Q000276:immunology; D003594:Cytoplasmic Granules / Q000276:immunology / Q000648:ultrastructure; D004195:Disease Models, Animal; D047091:Eosinophil Granule Proteins; D004804:Eosinophils / Q000276:immunology* / Q000473:pathology / Q000648:ultrastructure; D017353:Gene Deletion; D007274:Injections, Intraperitoneal; D008168:Lung / Q000276:immunology* / Q000473:pathology*; D008645:Mesocestoides / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D008854:Microscopy, Electron; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology; D012260:Ribonucleases
D000485:Allergens; D000947:Antigens, Helminth; D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D010047:Ovalbumin; D012260:Ribonucleases