[Expression of endothelin-1 and nitric oxide synthase mRNA in gastric mucosa of rats with cirrhosis and portal hypertensive gastropathy after disconnective operations].
OBJECTIVE
To investigate expression of endothelin-1 (ET-1) and nitric oxide synthase(NOS) gene in gastric mucosa in the rats with cirrhosis and portal hypertensive gastropathy (PHG) after disconnective operation.


METHODS
Wistar rats were divided into three groups: normal (n = 12), cirrhosis and PHG (n = 12), and post-disconnective (n = 12). The rat models were induced by intraperitonal injection of thiocetamide for 16 weeks. The third group rats were reared for 3 months after the operations. ET-1 and NOS mRNA from the gastric mucosa of the three groups were measured quantitatively by Dot blot technique.


RESULTS
ET-1 mRNA decreased in the PHG rats as compared to the nomal rats (P < 0.01). Further decrease was found in the post-disconnective rats (P < 0.01). NOS mRNA increased slightly in the PHG rats (P > 0.05) and increased obviously in the post-disconnective rats (P < 0.01).


CONCLUSIONS
After the cirrhosis and PHG rats were subjected to disconnective operations, ET-1 was insufficient and NOS was too much in the gastric mucosa. These resulted in the gastric mucosal vessel dilatation. The imbalance of ET/NO proportion could partly attribute to rebleeding after disconnection.
Guan
H
H
Department of General Surgery, First Affiliated Hospital of Suzhou Medical College.
Chen
Y
Y
Qian
H
H
D000818:Animals; D019332:Endothelin-1 / Q000096:biosynthesis* / Q000235:genetics; D005753:Gastric Mucosa / Q000378:metabolism*; D006975:Hypertension, Portal / Q000378:metabolism* / Q000601:surgery; D008106:Liver Cirrhosis, Experimental / Q000150:complications*; D008297:Male; D019001:Nitric Oxide Synthase / Q000096:biosynthesis*; D052250:Nitric Oxide Synthase Type III; D011170:Portasystemic Shunt, Surgical; D011184:Postoperative Period; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
D019332:Endothelin-1; D012333:RNA, Messenger; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat