[Mechanism of CD44V6 in human colorectal carcinoma metastasis].
OBJECTIVE
To study the mechanism of CD44V6 in human colorectal carcinoma (HCC) matastasis.


METHODS
The effects of CD44V6 on HCC cell adhering to base membrane and vascular endothelium were investigated by human amniotic membrane invasion model and co-culturing HCC cell with vascular endothelium. The influence of CD44V6 variants on cytoskeleton and gap junctional intercellular communication (GJIC) was studied by immunofluorescent confocal 3-dimensional reconstruction and fluorescence recovery after photobleaching technique.


RESULTS
CD44V6 antibody can decrease the ability of HCC cells to adhere to amniotic membrane and vascular endothelium. It also can affect the distribution, polymerization and depolymerization of actin of HCC cells, but does not significantly affect GJIC of HCC cells.


CONCLUSIONS
CD44V6 may play an important role in promoting HCC cells to adhere to vascular endothelium and base membranes and may affect the distribution, polymerization and depolymerization of actin in HCC cells and facilitate HCC cell metastasis.
Li
Z
Z
Department of Pathology, First Military Medical University, Guangzhou.
Ding
Y
Y
Deng
Y
Y
D018920:Coculture Techniques; D015179:Colorectal Neoplasms / Q000473:pathology*; D004730:Endothelium, Vascular / Q000473:pathology; D019073:HT29 Cells / Q000473:pathology; D006801:Humans; D018960:Hyaluronan Receptors; D009362:Neoplasm Metastasis; D014407:Tumor Cells, Cultured
D018960:Hyaluronan Receptors