title: Susceptibility of Biomphalaria tenagophila and Biomphalaria straminea to Schistosoma mansoni infection detected by low stringency polymerase chain reaction.
abstract: In order to determine Schistosoma mansoni infection rates in Biomphalaria tenagophila and B. straminea, low stringency polymerase chain reaction (LS-PCR) technique was used as a complementary method to light exposure technique. LS-PCR has already been standardized in our laboratory to detect the trematode DNA in B. glabrata. Higher S. mansoni infection rates were detected using conventional method and LS-PCR. The parasite DNA profile was detected in both species after 7-day exposure to miracidia, using LS-PCR. This technique enables early detection of schistosomiasis transmission focuses, in endemic areas, before the beginning of cercariae shedding.
authors.affiliation: Centro de Pesquisas René Rachou, FIOCRUZ, Laboratório de Malacologia, Belo Horizonte, MG, Brasil.
authors.forename: L K
authors.initials: LK
authors.lastname: Jannotti-Passos
authors.forename: C P
authors.initials: CP
authors.lastname: de Souza
mesh_terms: D000818:Animals; D001700:Biomphalaria / Q000469:parasitology*; D006790:Host-Parasite Interactions; D008027:Light; D016133:Polymerase Chain Reaction / Q000379:methods*; D012550:Schistosoma mansoni / Q000302:isolation & purification / Q000502:physiology*