title: Insertional mutagenesis as a route to identifying genes involved in self renewal of haemopoietic stem cells.
abstract: The genes controlling self renewal in the haemopoietic system are still unknown. Using retroviral insertional mutagenesis we have established multipotent haemopoietic stem cell lines (FDCP-mix) that possess an increased self renewal capacity in vitro. To identify genes involved in the regulation of self renewal, proviral integration sites were cloned from FDCP-mix cells and used as probes to screen independently isolated FDCP-mix cell lines for a common proviral insertion site. So far, two common integration sites have been identified, A25 and M4. A25 is rearranged in 50% of the FDCP-mix cell lines and M4 in 10%. Genes located at or near these sites are likely candidates for the control of self renewal of haemopoietic stem cells.
authors.affiliation: Paterson Institute for Cancer Research, Manchester, UK.
authors.forename: U
authors.initials: U
authors.lastname: Just
authors.forename: D
authors.initials: D
authors.lastname: Boettiger
authors.forename: O
authors.initials: O
authors.lastname: Kan
authors.forename: T M
authors.initials: TM
authors.lastname: Dexter
authors.forename: E
authors.initials: E
authors.lastname: Spooncer
mesh_terms: D002454:Cell Differentiation / Q000235:genetics*; D046508:Culture Techniques / Q000379:methods; D006378:Helper Viruses; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D016254:Mutagenesis, Insertional; D012190:Retroviridae; D016662:Virus Integration / Q000235:genetics