Identification of Escherichia coli flagellar types by restriction of the amplified fliC gene.
A total of 182 strains of Escherichia coli (133 reference strains, 22 clinical strains, nine nonmotile strains and 18 strains derived from K-12) were characterized by HhaI restriction of the amplified flagellin gene (fliC). The amplified fliC product was a single band between 0.9 and 2.6 kbp. With the collection of reference strains which represented 48 flagellar types (H-types), a total of 62 patterns (F-types) were observed after HhaI restriction. A single F-type was associated with each of 39 H-types and more than one F-type was associated with the other nine H-types. Antigenically related H-types 12 and 45 gave a single F-type. The determination of HhaI-fliC F-types could allow deduction of all H-types and subdivision of some of these. Application of this identification system to 22 E. coli clinical isolates yielded nine F-patterns and the deduced H-types were confirmed by serotyping in all cases. Nine nonmotile strains were studied and their F-types were also identified. The proposed determination of fliC restriction patterns should be helpful for epidemiological studies.
Machado
J
J
Centre national de référence pour le typage moléculaire entérique, unité des entérobactéries, unité Inserm 389, Institut Pasteur, Paris, France.
Grimont
F
F
Grimont
P A
PA
D015373:Bacterial Typing Techniques; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology; D005408:Flagellin / Q000235:genetics*; D005784:Gene Amplification; D005798:Genes, Bacterial; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length; D012703:Serotyping
D005408:Flagellin