title: Zinc concentration of selected ocular tissues in zinc-deficient rats.
abstract: A study was performed to determine the effect of zinc deficiency on the zinc concentration of the retina, lens, and the retinal pigment epithelium and choroid. Weanling, male Sprague-Dawley rats were fed ad libitum modified AIN-93 diets containing 3 mg zinc/kg diet (-Zn; n = 10) for 6 wk. Control animals were pair-fed (+ZnPF; n = 10) or fed ad libitum (+ZnAL; n = 10) diets containing 100 mg zinc/kg diet. At 6 wk, plasma and tibia zinc were measured by flame atomic absorption spectrophotometry to confirm zinc deficiency. The zinc concentration of ocular tissues was measured by inductively coupled plasma-mass spectrometry. Mean (+/- SEM) lens zinc concentration was significantly depressed in the zinc-deficient group as compared to that of pair-fed or ad libitum-fed controls, suggesting that the role of zinc in cataract formation should be investigated. The zinc concentration of total neural retina was preserved in zinc deficiency. Previously reported deterioration of retinal function in zinc deficiency may be the result of a decline in the zinc concentration of a specific cell layer of the retina that cannot be detected on gross analysis of the entire retina.
authors.affiliation: College of Pharmacy and Nutrition, University of Saskatchewan, Saskatoon, Canada.
authors.forename: J S
authors.initials: JS
authors.lastname: Fabe
authors.forename: B H
authors.initials: BH
authors.lastname: Grahn
authors.forename: P G
authors.initials: PG
authors.lastname: Paterson
mesh_terms: D000818:Animals; D004032:Diet; D005123:Eye / Q000737:chemistry*; D008297:Male; D013058:Mass Spectrometry; D009752:Nutritional Status; D051381:Rats; D017207:Rats, Sprague-Dawley; D013054:Spectrophotometry, Atomic; D015430:Weight Gain; D015032:Zinc / Q000737:chemistry / Q000172:deficiency* / Q000378:metabolism*
chemical_list: D015032:Zinc