title: [Analysis of nuclear 'trypsin-like' and 'trypsin inhibitor' coleoptile complexes at the post-embryonic stage of wheat ontogenesis].
abstract: "Inhibitor-trypsin" and "trypsin-like" complexes of the nuclear matrix of actively growing wheat coleoptiles and germinated wheat embryos were isolated using affine chromatography on columns with immobilized trypsin and trypsin inhibitor, respectively. No such complexes were found after the termination of the coleoptile growth or in the air-dry quiescent wheat embryos. Electron microscopy of the "trypsin-like" complexes has shown a rosette- and bead-like structure with a dense granular layer which was absent in the "inhibitor-trypsin" complexes. Electrophoretic analysis demonstrated a range of protein molecular masses from approximately 14 to approximately 67 kDa. DNA, RNA. Carbohydrates were also found in the complexes. Immunoenzymatic analysis visualized endogenous auxin in the complexes of 24-hour germinated wheat embryos.
authors.affiliation: Institute of Biology, Ufa Scientific Center of the Russian Academy of Sciences, Russia.
authors.forename: E G
authors.initials: EG
authors.lastname: Ivanova
authors.forename: G Kh
authors.initials: GKh
authors.lastname: Vafina
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism*; D002846:Chromatography, Affinity; D008854:Microscopy, Electron; D015530:Nuclear Matrix / Q000378:metabolism; D012639:Seeds / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D014908:Triticum / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D014357:Trypsin / Q000378:metabolism*; D014361:Trypsin Inhibitors / Q000378:metabolism*
chemical_list: D014361:Trypsin Inhibitors; D014357:Trypsin