Cloning and characterization of the human ameloblastin gene.
We isolated the full-length human ameloblastin (AMBN) cDNA clone using reverse transcription-polymerase chain reaction (RT-PCR) methods. Sequence analysis of the AMBN cDNA revealed an open reading frame of 1341bp encoding a 447-amino-acid protein. Comparison with pig, cattle, rat, and mouse AMBN sequences showed a high amino acid sequence similarity and led to the identification of a novel 78bp (26 amino acids) insert resulting from internal sequence duplication. By DNA analysis of a human genomic clones, the AMBN gene was shown to consist of 13 exons and a novel 78bp segment, which proved to comprise two small exons. Human ameloblastomas express AMBN transcripts that contain some mutations.
Toyosawa
S
S
Department of Oral Pathology, Osaka University Faculty of Dentistry, 1-8 Yamadaoka, Suita, 565-0871, Osaka, Japan. toyosawa@dent.osaka-u.ac.jp
Fujiwara
T
T
Ooshima
T
T
Shintani
S
S
Sato
A
A
Ogawa
Y
Y
Sobue
S
S
Ijuhin
N
N
D000564:Ameloblastoma / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D003746:Dental Enamel Proteins / Q000235:genetics*; D005091:Exons; D015972:Gene Expression Regulation, Neoplastic; D005796:Genes / Q000235:genetics*; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
C490889:AMBN protein, human; C490890:Ambn protein, mouse; D018076:DNA, Complementary; D003746:Dental Enamel Proteins; D004247:DNA