title: HPLC enantiomeric resolution of novel aromatase inhibitors on cellulose- and amylose-based chiral stationary phases under reversed phase mode.
abstract: The chiral resolution of seven aromatase inhibitors (four triazole derivatives (Ia, Ib, Ic, and Id) and three tetrazole derivatives (IIa, IIb, and IIc)) was achieved on Chiralcel OJ-R [cellulose tris (4-methyl benzoate)], Chiralcel OD-RH [cellulose tris (3,5-dimethylphenyl carbamate)], and Chiralpak AD-RH [amylose tris (3,5-dimethylphenyl carbamate)] chiral stationary phases. The mobile phases used were A: 2-PrOH-MeCN (90:10, v/v); B: 2-PrOH-MeCN (50:50, v/v); C: MeCN-H(2)O (50:50, v/v); D: MeCN-H(2)O (80:20, v/v); and E: MeCN-H(2)O (95:05, v/v). The flow rate was 0.5 mL/min for all the mobile phases. The resolution capability of these chiral stationary phases were in the order Chiralpak AD-RH > Chiralcel OD-RH > Chiralcel OJ-R. The values of alpha and Rs of the resolved enantiomers of the aromatase inhibitors varied from 1.02-5.63 and 1. 12-6.72, respectively.
authors.affiliation: Pharmaceutical Analysis Laboratory, Biological and Medical Research Department (MBC-03), King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia. enein@kfshrc.edu.sa
authors.forename: H Y
authors.initials: HY
authors.lastname: Aboul-Enein
authors.forename: I
authors.initials: I
authors.lastname: Ali
authors.forename: G
authors.initials: G
authors.lastname: Gübitz
authors.forename: C
authors.initials: C
authors.lastname: Simons
authors.forename: P J
authors.initials: PJ
authors.lastname: Nicholls
mesh_terms: D000688:Amylose; D047072:Aromatase Inhibitors; D002482:Cellulose; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D013237:Stereoisomerism; D013777:Tetrazoles / Q000737:chemistry* / Q000302:isolation & purification; D014230:Triazoles / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D047072:Aromatase Inhibitors; D004791:Enzyme Inhibitors; D013777:Tetrazoles; D014230:Triazoles; D002482:Cellulose; D000688:Amylose