Effect of estrogen and progesterone on gene expression of growth regulatory molecules and proto-oncogene in vascular smooth muscle cells.
We examined influences of estrogen and progestogen on gene expression of the growth regulatory molecules: platelet-derived growth factor (PDGF), interleukin-1 (IL-1), interleukin-6 (IL-6) and proto-oncogene c-myc in vascular smooth muscle cells (VSMC) by reverse transcription-polymerase chain reaction (RT-PCR) and Southern-blotting. VSMC were exposed to estrone-sulfate (E1-S) and medroxyprogesterone acetate (MPA) to induce differentiation. E1-S inhibited the expression of PDGF-A chain, IL-1, IL-6 and c-myc mRNA, whereas MPA had no effect. Inhibition by E1-S was not affected by treatment combined with MPA. These findings suggest that estrogen modulates these growth regulatory molecules and c-myc gene expression in VSMC but not progestogen. We concluded that estrogen may have a direct atheroprotective effect through inhibition of growth regulatory factors.
Okubo
T
T
Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Japan.
Urabe
M
M
Tsuchiya
H
H
Iwasa
K
K
Yokota
K
K
Kikuchi
N
N
Yamamoto
T
T
Honjo
H
H
D001161:Arteriosclerosis / Q000209:etiology; D015139:Blotting, Southern; D002478:Cells, Cultured; D004347:Drug Interactions; D004970:Estrone / Q000031:analogs & derivatives* / Q000494:pharmacology; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis*; D015850:Interleukin-6 / Q000096:biosynthesis*; D017258:Medroxyprogesterone Acetate / Q000494:pharmacology*; D024510:Muscle Development; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000254:growth & development*; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis*; D000090063:Proto-Oncogene Mas; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D007375:Interleukin-1; D015850:Interleukin-6; C000715470:MAS1 protein, human; D010982:Platelet-Derived Growth Factor; D000090063:Proto-Oncogene Mas; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D004970:Estrone; D017258:Medroxyprogesterone Acetate; C017296:estrone sulfate