Intranasal immunization with mumps virus DNA vaccine delivered by influenza virosomes elicits mucosal and systemic immunity.
To improve the efficiency of liposome-mediated DNA transfer as a tool for gene therapy or vaccinology, we have further developed a new delivery system based on the modified immunopotentiating reconstituted influenza virus (IRIV). In this study, we engineered a plasmid DNA vector expressing the mumps virus hemagglutinin or the fusion protein. The administration of this DNA vaccine delivered by influenza virosomes, in combination with the mucosal adjuvant Escheriagen via the intranasal route, was efficient for inducing an immune response, both mucosally and systemically, in mice. The production of IgG2a mumps virus-specific antibodies and the secretion of interleukin 10 (IL-10) by antigen-specific T cells indicated that not only Th1 but also Th2 responses were induced by this DNA vaccine formulation. These results suggest that cationic virosomes in combination with Escheriagen may have great potential as an efficient delivery system for intranasal DNA immunization and provide an immune barrier at the mucosal sites.
Cusi
M G
MG
Department of Molecular Biology, Section of Microbiology, University of Siena, Via Laterina 8, Siena, 53100, Italy. cusi@unisi.it
Zurbriggen
R
R
Valassina
M
M
Bianchi
S
S
Durrer
P
P
Valensin
P E
PE
Donati
M
M
Glück
R
R
D000281:Administration, Intranasal; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000917:Antibody Formation; D002522:Chlorocebus aethiops; D005260:Female; D018014:Gene Transfer Techniques; D018928:Immunity, Mucosal; D016753:Interleukin-10 / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D009107:Mumps / Q000276:immunology* / Q000517:prevention & control; D009108:Mumps Vaccine / Q000008:administration & dosage*; D009109:Mumps virus / Q000235:genetics / Q000276:immunology*; D009975:Orthomyxoviridae; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage*; D014709:Vero Cells
D000914:Antibodies, Viral; D009108:Mumps Vaccine; D019444:Vaccines, DNA; D016753:Interleukin-10