Structural and functional similarities between HIV-1 reverse transcriptase and the Escherichia coli RNA polymerase beta' subunit.
Four monoclonal antibodies (MAbs) recognizing HIV-1 reverse transcriptase (RT) were shown here to cross-react with the beta' subunit of Escherichia coli RNA polymerase (RNAP). The anti-RT MAbs bind to a peptide comprising residues 294-305 of the RT amino acid sequence. Computer analyses revealed sequence similarity between this peptide and two regions of the RNAP beta' subunit. MAb-binding studies using RT mutants suggested that the epitope is located to amino acids 652-663 of the beta' sequence. One of the MAbs which inhibited the polymerase activity of RT also mediated a dose dependent inhibition of the RNAP activity.
Szilvay
A M
AM
Department of Molecular Biology, University of Bergen, HIB, P.O. Box 7800, N-5020, Bergen, Norway. anne.szilvay@mbi.uib.no
Stern
B
B
Blichenberg
A
A
Helland
D E
DE
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D001665:Binding Sites; D015153:Blotting, Western; D002384:Catalysis / Q000187:drug effects; D003429:Cross Reactions / Q000276:immunology*; D012321:DNA-Directed RNA Polymerases / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D000939:Epitopes / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D004926:Escherichia coli / Q000201:enzymology*; D054303:HIV Reverse Transcriptase / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D015497:HIV-1 / Q000201:enzymology*; D007700:Kinetics; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D011487:Protein Conformation; D021122:Protein Subunits; D013329:Structure-Activity Relationship
D000911:Antibodies, Monoclonal; D000939:Epitopes; D010446:Peptide Fragments; D021122:Protein Subunits; D054303:HIV Reverse Transcriptase; D012321:DNA-Directed RNA Polymerases