Characterization of auxin-induced ARRO-1 expression in the primary root of Malus domestica.
ARRO-1, a novel 2-oxoacid-dependent dioxygenase (2-ODD) is up-regulated during IBA-induced adventitious root formation in stem discs of Malus domestica. Analysis of ARRO-1's expression profile in the primary root of apple seedlings indicates that it is also highly up-regulated in the root in response to both IAA and IBA, but not 2, 4-D. Auxin-derived evolution of ethylene can be discounted as the source of ARRO-1 induction as ARRO-1 is not induced in the root following treatment with the ethylene precursor ACC. Constitutive expression in the primary root further suggests that ARRO-1's role may be linked to the regulation of natural auxin levels within plant tissues. Significantly, orthologues of ARRO-1 have been identified in Arabidopsis thaliana by means of DNA database analysis which will enable the further molecular characterization of this class of 2-ODD.
Butler
E D
ED
Department of Botany, University College Dublin, Belfield, Dublin 4, Ireland. eoin.butler@ucd.ie
Gallagher
T F
TF
D000595:Amino Acid Sequence; D018744:DNA, Plant; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D007210:Indoleacetic Acids / Q000494:pharmacology*; D008969:Molecular Sequence Data; D010105:Oxygenases / Q000737:chemistry / Q000235:genetics*; D018517:Plant Roots / Q000235:genetics; D019685:Rosales / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D018744:DNA, Plant; D007210:Indoleacetic Acids; D010105:Oxygenases; C419509:adventitous rooting related oxygenase