Aggregates of cAMP-dependent kinase RIalpha characterize a type of cholinergic neurons in the rat brain.
Acetylcholine is synthesized by different types of neurons, showing a distinct biochemical phenotype. Aggregates of RIalpha regulatory subunit of cAMP-dependent protein kinases are visualized by immunohistochemistry only in some cholinergic neurons, since they tightly colocalize with two different markers, choline acetyltransferase (ChAT) and vesicular acetylcholine transporter (VAChT). These neurons are present mainly in brain areas related to the limbic system. None of the other regulatory subunits of cAMP dependent kinases colocalize with cholinergic markers.
Mucignat-Caretta
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Dipartimento di Anatomia e Fisiologia Umana, Universita' di Padova, Padova, Italy. mucignat@ux1.unipd.it
D000679:Amygdala / Q000737:chemistry / Q000166:cytology / Q000201:enzymology; D000818:Animals; D001921:Brain / Q000166:cytology* / Q000201:enzymology*; D002352:Carrier Proteins / Q000032:analysis; D002795:Choline O-Acetyltransferase / Q000032:analysis; D002799:Cholinergic Fibers / Q000737:chemistry / Q000201:enzymology*; D054756:Cyclic AMP-Dependent Protein Kinase RIalpha Subunit; D017868:Cyclic AMP-Dependent Protein Kinases / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D008297:Male; D026901:Membrane Transport Proteins; D051381:Rats; D012154:Reticular Formation / Q000737:chemistry / Q000166:cytology / Q000201:enzymology; D013377:Substantia Innominata / Q000737:chemistry / Q000166:cytology / Q000201:enzymology; D050494:Vesicular Acetylcholine Transport Proteins; D033921:Vesicular Transport Proteins
D002352:Carrier Proteins; D054756:Cyclic AMP-Dependent Protein Kinase RIalpha Subunit; D026901:Membrane Transport Proteins; C517836:Prkar1a protein, rat; C493461:Slc18a3 protein, rat; D050494:Vesicular Acetylcholine Transport Proteins; D033921:Vesicular Transport Proteins; D002795:Choline O-Acetyltransferase; D017868:Cyclic AMP-Dependent Protein Kinases