title: Continuous production of lacticin 3147 and nisin using cells immobilized in calcium alginate.
abstract: Bacteriocinogenic strains, Lactococcus lactis subsp. lactis DPC 3147 and L. lactis DPC 496, producing lacticin 3147 and nisin, respectively, were immobilized in double-layered calcium alginate beads. These beads were inoculated into MRS broth at a ratio of 1:4 and continuously fermented for 180 h. Free cells were used to compare the effect of immobilization on bacteriocin production. After equilibrium was reached, a flow rate of 580 ml h(-1) was used in the immobilized cell (IC), and 240 ml h(-1) in free-cell (FC) bioreactors. Outgrowth from beads was observed after 18 h. Bacteriocin production peaked at 5120 AU ml(-1) in both IC and FC bioreactors. However, FC production declined after 80 h to 160 AU ml(-1) at the end of the fermentation. Results of this study indicate that immobilization offers the possibility of a more stable and long-term means of producing lacticin 3147 in laboratory media than with free cells.
authors.affiliation: Department of Food Technology, National Food Biotechnology Centre, University College, Cork, Ireland. a.scannell@ucc.ie
authors.forename: A G
authors.initials: AG
authors.lastname: Scannell
authors.forename: C
authors.initials: C
authors.lastname: Hill
authors.forename: R P
authors.initials: RP
authors.lastname: Ross
authors.forename: S
authors.initials: S
authors.lastname: Marx
authors.forename: W
authors.initials: W
authors.lastname: Hartmeier
authors.forename: E K
authors.initials: EK
authors.lastname: Arendt
mesh_terms: D000464:Alginates; D000818:Animals; D001426:Bacterial Proteins / Q000096:biosynthesis; D001430:Bacteriocins / Q000096:biosynthesis*; D001672:Biocompatible Materials; D004058:Diffusion; D005947:Glucose / Q000378:metabolism; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D019344:Lactic Acid / Q000096:biosynthesis; D013294:Lactococcus lactis / Q000254:growth & development / Q000378:metabolism*; D009561:Nisin / Q000096:biosynthesis*
chemical_list: D000464:Alginates; D001426:Bacterial Proteins; D001430:Bacteriocins; D001672:Biocompatible Materials; D006603:Hexuronic Acids; C082508:lacticin 481; D009561:Nisin; D019344:Lactic Acid; D020723:Glucuronic Acid; D005947:Glucose