Heating of food and haemoglobin adducts from carcinogens: possible precursor role of glycidol.
Studies of adducts from reactive compounds to haemoglobin (Hb) by gas chromatography-tandem mass spectrometry according to the N-alkyl Edman method reveals the occurrence of N-(2,3-dihydroxypropyl)valine (diHOPrVal) at levels of 1-2 pmol/g Hb, in persons without known exposure. The hypothesis that this background originates from glycidol or related compounds during heating of food was tested in experiments with rats. Animals fed fried animal feed for 30 or 72 days showed an increase of the diHOPrVal level by about 50% compared with controls. Several arguments, such as the formation of reactive oxiranes by heat-induced dehydration of glycol configurations in glycerol and sugars, support the idea that glycidol (or e.g. glycidyl esters) are precursors of the adduct. In Hb samples, reduced for stabilisation of aldehyde adducts, relatively high levels of adducts determined as diHOPrVal were found, although without significant relation to frying of the feed. There is thus no indication that reduction in vivo of, for example, the Schiff base from glyceraldehyde, is a pathway for formation of the diHOPrVal. The background level of diHOPrVal in humans Hb is low, and the cancer risk associated with exposure to the specific alkylator-probably glycidol-formed in cooking, is therefore presumably low. The result implies, however, that low-molecular mass mutagenic oxiranes formed during the heating of food should be studied further.
Hindsø Landin
H
H
Dept of Molecular Genome Research, Stockholm University, SE 10691, Stockholm, Sweden.
Tareke
E
E
Rydberg
P
P
Olsson
U
U
Törnqvist
M
M
D000818:Animals; D002273:Carcinogens / Q000378:metabolism*; D003296:Cooking; D004032:Diet; D004852:Epoxy Compounds / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006454:Hemoglobins / Q000378:metabolism*; D006358:Hot Temperature; D008297:Male; D020005:Propanols / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014633:Valine / Q000031:analogs & derivatives / Q000032:analysis
D002273:Carcinogens; D004852:Epoxy Compounds; D006454:Hemoglobins; C416044:N-(2,3-dihydroxypropyl)valine; D020005:Propanols; D014633:Valine; C004312:glycidol