Comparative evaluation of four detectors in the high-performance liquid chromatographic analysis of chiral nonaromatic alcohols.
A comparative evaluation of ultraviolet, polarimetric, refractive index, and evaporative light-scattering detection coupled with high-performance liquid chromatography has been developed for the separation and quantitation of the enantiomers of chiral nonaromatic alcohols, some of which are intermediates in the synthesis of chiral drugs. (R,S)-3-tert-butylamino-1,2-propanediol; (R,S)-glycidol; and (R,S)-1-(4-morpholino)-2-octanol are selected as model compounds in order to compare the detection sensitivity and the linearity of the response with the four detectors. Separation of the enantiomers is performed using chiral stationary phases in normal-phase liquid chromatography. A one-day validation is achieved for (S)-3-tert-butylamino-1,2-propanediol with each detector, and limits of quantitation are determined for the three compounds. Advantages and limitations of the four detectors are discussed.
Toussaint
B
B
Department of Analytical Pharmaceutical Chemistry, Institute of Pharmacy, University of Liège, Belgium. B.Toussaint@ulg.ac.be
Duchateau
A L
AL
van der Wal
S
S
Albert
A
A
Hubert
P
P
Crommen
J
J
D000438:Alcohols / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis / Q000379:methods*; D013237:Stereoisomerism
D000438:Alcohols