title: High-throughput scanning of the rat genome using interspersed repetitive sequence-PCR markers.
abstract: We report the establishment of a hybridization-based marker system for the rat genome based on the PCR amplification of interspersed repetitive sequences (IRS). Overall, 351 IRS markers were mapped within the rat genome. The IRS marker panel consists of 210 nonpolymorphic and 141 polymorphic markers that were screened for presence/absence polymorphism patterns in 38 different rat strains and substrains that are commonly used in biomedical research. The IRS marker panel was demonstrated to be useful for rapid genome screening in experimental rat crosses and high-throughput characterization of large-insert genomic library clones. Information on corresponding YAC clones is made available for this IRS marker set distributed over the whole rat genome. The two existing rat radiation hybrid maps were integrated by placing the IRS markers in both maps. The genetic and physical mapping data presented provide substantial information for ongoing positional cloning projects in the rat.
authors.affiliation: Max-Planck Institute of Molecular Genetics, Ihnestrasse 73, Berlin-Dahlem, D-14195, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Gösele
authors.forename: L
authors.initials: L
authors.lastname: Hong
authors.forename: T
authors.initials: T
authors.lastname: Kreitler
authors.forename: M
authors.initials: M
authors.lastname: Rossmann
authors.forename: B
authors.initials: B
authors.lastname: Hieke
authors.forename: U
authors.initials: U
authors.lastname: Gross
authors.forename: M
authors.initials: M
authors.lastname: Kramer
authors.forename: H
authors.initials: H
authors.lastname: Himmelbauer
authors.forename: M T
authors.initials: MT
authors.lastname: Bihoreau
authors.forename: A E
authors.initials: AE
authors.lastname: Kwitek-Black
authors.forename: S
authors.initials: S
authors.lastname: Twigger
authors.forename: P J
authors.initials: PJ
authors.lastname: Tonellato
authors.forename: H J
authors.initials: HJ
authors.lastname: Jacob
authors.forename: L C
authors.initials: LC
authors.lastname: Schalkwyk
authors.forename: K
authors.initials: K
authors.lastname: Lindpaintner
authors.forename: D
authors.initials: D
authors.lastname: Ganten
authors.forename: H
authors.initials: H
authors.lastname: Lehrach
authors.forename: M
authors.initials: M
authors.lastname: Knoblauch
mesh_terms: D000818:Animals; D002460:Cell Line; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D006224:Cricetinae; D005819:Genetic Markers; D016678:Genome; D020071:Interspersed Repetitive Sequences; D016133:Polymerase Chain Reaction / Q000379:methods; D051381:Rats; D011916:Rats, Inbred F344 / Q000235:genetics; D011919:Rats, Inbred Strains / Q000235:genetics*
chemical_list: D005819:Genetic Markers