title: Ordering the final events in yeast exocytosis.
abstract: In yeast, assembly of exocytic soluble N-ethylmaleimide-sensitive fusion protein (NSF) attachment protein receptor (SNARE) complexes between the secretory vesicle SNARE Sncp and the plasma membrane SNAREs Ssop and Sec9p occurs at a late stage of the exocytic reaction. Mutations that block either secretory vesicle delivery or tethering prevent SNARE complex assembly and the localization of Sec1p, a SNARE complex binding protein, to sites of secretion. By contrast, wild-type levels of SNARE complexes persist in the sec1-1 mutant after a secretory block is imposed, suggesting a role for Sec1p after SNARE complex assembly. In the sec18-1 mutant, cis-SNARE complexes containing surface-accessible Sncp accumulate in the plasma membrane. Thus, one function of Sec18p is to disassemble SNARE complexes on the postfusion membrane.
authors.affiliation: Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
authors.forename: E
authors.initials: E
authors.lastname: Grote
authors.forename: C M
authors.initials: CM
authors.lastname: Carr
authors.forename: P J
authors.initials: PJ
authors.lastname: Novick
mesh_terms: D000251:Adenosine Triphosphatases; D002352:Carrier Proteins / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D016764:Cell Polarity; D005089:Exocytosis; D005656:Fungal Proteins / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000378:metabolism; D020662:Guanine Nucleotide Exchange Factors; D008561:Membrane Fusion; D008565:Membrane Proteins / Q000378:metabolism*; D008954:Models, Biological; D051938:Munc18 Proteins; D052066:N-Ethylmaleimide-Sensitive Proteins; D009419:Nerve Tissue Proteins / Q000378:metabolism; D050765:Qa-SNARE Proteins; D050683:R-SNARE Proteins; D050600:SNARE Proteins; D029701:Saccharomyces cerevisiae Proteins; D022161:Transport Vesicles / Q000378:metabolism; D033921:Vesicular Transport Proteins; D015003:Yeasts
chemical_list: D002352:Carrier Proteins; D005656:Fungal Proteins; D020662:Guanine Nucleotide Exchange Factors; D008565:Membrane Proteins; D051938:Munc18 Proteins; D009419:Nerve Tissue Proteins; D050765:Qa-SNARE Proteins; D050683:R-SNARE Proteins; C064456:SEC2 protein, S cerevisiae; D050600:SNARE Proteins; C083241:SNC1 protein, S cerevisiae; C083242:SNC2 protein, S cerevisiae; C084006:SSO1 protein, S cerevisiae; C084007:SSO2 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D033921:Vesicular Transport Proteins; D000251:Adenosine Triphosphatases; D019204:GTP-Binding Proteins; C064404:SEC18 protein, S cerevisiae; D052066:N-Ethylmaleimide-Sensitive Proteins