Photodetection with 5-Aminolevulinic acid-induced protoporphyrin IX in the rat abdominal cavity: drug-dose-dependent fluorescence kinetics.
In 75% of cases, ovarian carcinoma has already metastasized in the abdominal cavity at the time of diagnosis. For determination of the necessity for a supplementary therapy, in addition to surgical resection, it is important to localize and stage microscopical intraperitoneal metastases of the tumor. Intraperitoneal photodetection of tumor metastases is based on preferential tumor distribution of a fluorescent tumor marker. The time-dependent differences in drug concentration between tumor and normal (T/N) tissues can be used to visualize small tumors. We performed fluorescence measurements on abdominal organs and tumor in the peritoneal cavity of rats. 5-Aminolevulinic acid (ALA)-induced protoporphyrin IX (PpIX) was used as the fluorescent marker. Three different drug doses (100, 25 and 5 mg/kg) were used and PpIX fluorescence profiles were followed up to 24 h after intravenous administration. Maximum T/N ratios were found 2-3 h after administration of ALA with all drug doses. A significant T/N tissue contrast was obtained for all abdominal organs tested after administration of 5 mg/kg.
Aalders
M C
MC
Laser Center, Academic Medical Center, Amsterdam, The Netherlands.
Sterenborg
H J
HJ
Stewart
F A
FA
van der Vange
N
N
D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D005456:Fluorescent Dyes / Q000493:pharmacokinetics*; D019804:Mesalamine / Q000493:pharmacokinetics*; D009368:Neoplasm Transplantation; D010534:Peritoneal Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000556:secondary; D011524:Protoporphyrins / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D013050:Spectrometry, Fluorescence; D014018:Tissue Distribution
D005456:Fluorescent Dyes; D011524:Protoporphyrins; D019804:Mesalamine; C028025:protoporphyrin IX