Radiosensitivity of HCV cells and their v-ras and v-raf transfectants.
In the present work, it was found that transfection of cultivated urothelial cells HCV-29 with v-raf and v-ras oncogenes increased their sensitivity to ionizing radiation, as documented by clonogenic studies. Flow cytometry study showed, that HCV-29 and their v-ras transfectants were arrested around middle S phase, whereas v-raf transfectants randomly at each point of S phase. This unusual reaction of HCV-29 v-raf cells may partially explain studies of P21(WAF1/CIP1) and GADD45 genes, whose transcripts were found only in these cells. Increased radiosensitivity of v-ras transfectants is probably associated with c-JUN protein overexpression. Altogether the obtained results suggested different mechanism of reaction on irradiation of v-raf and v-ras transfected cells.
Kowalczyk
D
D
Department of Cell Biology, The Maria Sklodowska Memorial Cancer Center, 02-781, W.K. Roentgen 5, Warsaw, Poland.
Skierski
J
J
Koronkiewicz
M
M
Janik
P
P
D001483:Base Sequence; D002453:Cell Cycle; D002460:Cell Line; D017931:DNA Primers; D005434:Flow Cytometry; D011905:Genes, ras; D006801:Humans; D027384:Oncogene Proteins v-raf; D011836:Radiation Tolerance / Q000235:genetics*; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
D017931:DNA Primers; D015682:Retroviridae Proteins, Oncogenic; D027384:Oncogene Proteins v-raf