Multicenter evaluation of the new HIV DUO assay for simultaneous detection of HIV antibodies and p24 antigen.
A multicenter survey was performed to evaluate a new semi-automated human immunodeficiency virus fourth generation antibodies and antigen simultaneous assay. This assay showed a sensitivity of 100% and specificity of 99.6% among sera obtained from hospitalized patients or blood donors. Sera obtained from commercially available as well as in-house seroconversions were tested showing that HIV DUO is able to reveal an infected state in 11 out of 14 cases earlier than conventional tests. This new assay improves old test performances in terms of sensitivity, maintaining specificity at very high levels.
Portincasa
P
P
Dipartimento di Patologia e Medicina di Laboratorio, Università degli Studi di Parma.
Grillo
R
R
Pauri
P
P
Colao
M G
MG
Valcavi
P P
PP
Speziale
D
D
Mazzarelli
G
G
De Majo
E
E
Varaldo
P E
PE
Fadda
G
G
Chezzi
C
C
Dettori
G
G
D015492:AIDS Serodiagnosis / Q000379:methods*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D015483:HIV Antibodies / Q000097:blood*; D016655:HIV Core Protein p24 / Q000097:blood*; D006679:HIV Seropositivity / Q000175:diagnosis; D006801:Humans
D015483:HIV Antibodies; D016655:HIV Core Protein p24