MHC antigen expression in human first trimester spinal cord with implications for clinical transplantation procedures.
We report human leukocyte antigen (HLA) class I expression in 5-17% and class II in 0-9% of first trimester human spinal cord cells. After 8 days in culture with gamma-interferon, >87% of the spinal cord cells expressed HLA class II. However, mixed cultures of adult human peripheral lymphocytes and immature human spinal cord cells, showed no induction of lymphocyte proliferation prior to or after gamma-interferon exposure in culture. In conclusion, we report non-immunogenic expression of HLA antigens in the human first trimester spinal cord.
Akesson
E
E
Department of NEUROTEC, Karolinska Institutet, Huddinge University Hospital, KFC 4th floor, S-141 86, Novum, Sweden. elisabet.akesson@neurotec.ki.se
Markling
L
L
Kjaeldgaard
A
A
Falci
S
S
Ringdén
O
O
D000970:Antineoplastic Agents / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects / Q000276:immunology; D002478:Cells, Cultured; D016332:Fetal Tissue Transplantation / Q000276:immunology*; D005333:Fetus / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D005865:Gestational Age; D015395:Histocompatibility Antigens Class I / Q000032:analysis / Q000096:biosynthesis*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D013116:Spinal Cord / Q000196:embryology / Q000276:immunology* / Q000637:transplantation*
D000970:Antineoplastic Agents; D015395:Histocompatibility Antigens Class I; D007371:Interferon-gamma