title: Fusions to maltose-binding protein: control of folding and solubility in protein purification.
authors.affiliation: University of Minnesota Cancer Center, Minneapolis 55455, USA.
authors.forename: D
authors.initials: D
authors.lastname: Sachdev
authors.forename: J M
authors.initials: JM
authors.lastname: Chirgwin
mesh_terms: D018528:ATP-Binding Cassette Transporters; D000595:Amino Acid Sequence; D000818:Animals; D019677:Artificial Gene Fusion / Q000379:methods*; D016282:Aspartic Acid Endopeptidases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D002402:Cathepsin D / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D003001:Cloning, Molecular / Q000379:methods; D004792:Enzyme Precursors / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D015951:Factor Xa; D005822:Genetic Vectors; D007202:Indicators and Reagents; D058619:Maltose-Binding Proteins; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins; D016133:Polymerase Chain Reaction / Q000379:methods; D017510:Protein Folding; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000302:isolation & purification; D012995:Solubility
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D002352:Carrier Proteins; D004792:Enzyme Precursors; D029968:Escherichia coli Proteins; D007202:Indicators and Reagents; D058619:Maltose-Binding Proteins; D009004:Monosaccharide Transport Proteins; D011993:Recombinant Fusion Proteins; C461599:maltose transport system, E coli; D015951:Factor Xa; D016282:Aspartic Acid Endopeptidases; C058345:procathepsin D; D002402:Cathepsin D