title: [Cloning and sequencing of the maize ribosome-inactivating protein gene].
abstract: A gene encoding maize Ribosome-inactivating protein was amplified by means of PCR using mRNA of maize leaves as a template, and cloned into pUC19 vector. The amplified DNA sequence has been determined, which consists of 828 bp and encodes 275 amino acid residues. Comparison with previously reported sequence shows 98.4% homologies in nucleotide sequence and 97.4% in amino acid sequence, respectively.
authors.affiliation: Institute of Bio-chemistry Engineering, Dalian University of Technology.
authors.forename: Q
authors.initials: Q
authors.lastname: Fan
authors.forename: X R
authors.initials: XR
authors.lastname: Gao
authors.forename: W L
authors.initials: WL
authors.lastname: Li
authors.forename: L J
authors.initials: LJ
authors.lastname: Jin
authors.forename: L J
authors.initials: LJ
authors.lastname: An
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D054790:Ribosome Inactivating Proteins, Type 2; D012270:Ribosomes / Q000187:drug effects*; D003313:Zea mays / Q000737:chemistry*
chemical_list: D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2; C093156:ribosome-inactivating protein 2, maize; D004247:DNA