Ferric nitrilotriacetate induced DNA and protein damage: inhibitory effect of a fermented papaya preparation.
The carcinogen Fe-NTA catalyzes the hydrogen peroxide-derived production of free radicals and possibly acts through a mechanism involving oxidative stress. Fermented papaya preparation (FPP) has been reported as a natural antioxidant able to prevent lipid peroxidation in vitro and in vivo. However, little is known about the antioxidant properties of FPP regarding iron-mediated oxidative damage to DNA and proteins. In the present study FPP protected supercoiled plasmid DNA against Fe-NTA plus H2O2 induced single and double strand breaks. Similar protective effects of FPP were evident when human T-lymphocytes were challenged with Fe-NTA/H2O2 and DNA damage was determined using the Comet assay. Fe-NTA/H2O2 also induced fragmentation of bovine serum albumin (BSA) in vitro and depleted cellular GSH levels in lymphocytes. BSA fragmentation and GSH depletion were dose-dependently counteracted by FPP. EPR spin trapping studies demonstrated that antioxidant properties of FPP are related to both hydroxyl scavenging as well as iron chelating properties.
Rimbach
G
G
Department of Molecular and Cell Biology, University of California, Berkeley 94720-3200, USA.
Guo
Q
Q
Akiyama
T
T
Matsugo
S
S
Moini
H
H
Virgili
F
F
Packer
L
L
D000818:Animals; D002273:Carcinogens / Q000494:pharmacology*; D002417:Cattle; D004249:DNA Damage; D004278:DNA, Superhelical / Q000187:drug effects; D005285:Fermentation; D005290:Ferric Compounds / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000378:metabolism*; D005638:Fruit / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D019169:Jurkat Cells; D009153:Mutagens / Q000494:pharmacology*; D009571:Nitrilotriacetic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D016877:Oxidants / Q000494:pharmacology; D010936:Plant Extracts; D010957:Plasmids / Q000187:drug effects; D012710:Serum Albumin, Bovine / Q000187:drug effects*; D013601:T-Lymphocytes / Q000187:drug effects; D014407:Tumor Cells, Cultured
D002273:Carcinogens; D004278:DNA, Superhelical; D005290:Ferric Compounds; D016166:Free Radical Scavengers; D009153:Mutagens; D016877:Oxidants; D010936:Plant Extracts; D012710:Serum Albumin, Bovine; D006861:Hydrogen Peroxide; D005978:Glutathione; D009571:Nitrilotriacetic Acid; C020326:ferric nitrilotriacetate