title: Testing the potential of flaxseed to affect spermatogenesis: morphometry.
abstract: Quantitative information was collected on male reproductive effects of maternal and postnatal dietary exposure to flaxseed (20 or 40%), flaxmeal (13 or 26%) or standard NIH AIN-93 feed (0% flaxseed control). Measurements were made on the testes of F1 generation males rats (1) whose mothers were exposed to the diets designated above, and (2) who, after weaning, were placed on the same diet as their mothers for an additional 70 days. The seminiferous tubules comprised 86%, 84%, 84%, 84% and 85% of the total testis volume while the interstitial space comprised 12%, 14%, 14%, 14%, 13% of the total testis volume for the 0% flaxseed/flaxmeal, 20% flaxseed, 13% flaxmeal, 40% flaxseed and 26% flaxmeal groups, respectively. Statistically significant decreases in the absolute volume of the seminiferous tubules were observed in the 20% and 40% flaxseed-treated groups when these groups were compared to controls. Borderline statistically significant differences were also observed when Sertoli cell nucleolar number per tubular cross-section were compared in the 13% flaxmeal and 20% flaxseed treatment groups. These effects were not considered biologically significant because other parameters of male reproductive function appeared normal. Overall, the quantitative information obtained suggests that exposure to flaxseed/flaxmeal at the doses used in the present study does not adversely affect testis structure or spermatogenesis in the rat.
authors.affiliation: Division of Toxicological Research, Center for Food Safety Applied Nutrition, Food and Drug Administration, Beltsville, MD 20708, USA. RLS@vm.cfsan.fda.gov
authors.forename: R L
authors.initials: RL
authors.lastname: Sprando
authors.forename: T F
authors.initials: TF
authors.lastname: Collins
authors.forename: P
authors.initials: P
authors.lastname: Wiesenfeld
authors.forename: U S
authors.initials: US
authors.lastname: Babu
authors.forename: C
authors.initials: C
authors.lastname: Rees
authors.forename: T
authors.initials: T
authors.lastname: Black
authors.forename: N
authors.initials: N
authors.lastname: Olejnik
authors.forename: J
authors.initials: J
authors.lastname: Rorie
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004032:Diet; D005260:Female; D019597:Flax / Q000737:chemistry*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D012639:Seeds / Q000737:chemistry; D012671:Seminiferous Tubules / Q000187:drug effects / Q000473:pathology; D013091:Spermatogenesis / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects* / Q000648:ultrastructure*; D013737:Testis / Q000187:drug effects / Q000473:pathology; D016707:Tissue Fixation