title: Crystallization and preliminary X-ray diffraction analysis of a eumenine mastoparan toxin: a new class of mast-cell degranulating peptide in the wasp venom.
abstract: Mastoparans are tetradecapeptides found to be the major component of vespid venoms. A mastoparan toxin isolated from the venom of Anterhynchium flavomarginatum micado has been crystallized and X-ray diffraction data collected to 2.7 A resolution using a synchrotron-radiation source. Crystals were determined to belong to the space group P6(2)22 (P6(4)22). This is the first mastoparan to be crystallized and will provide further insights into the conformational significance of mastoparan toxins with respect to their potency and activity in G-protein regulation.
authors.affiliation: Departamento de Física, IBILCE, UNESP, CP 136, CEP 15054-000, São José Rio Preto, SP, Brazil.
authors.forename: F
authors.initials: F
authors.lastname: Canduri
authors.forename: P
authors.initials: P
authors.lastname: Delatorre
authors.forename: V
authors.initials: V
authors.lastname: Fadel
authors.forename: C C
authors.initials: CC
authors.lastname: Lorenzi
authors.forename: J H
authors.initials: JH
authors.lastname: Pereira
authors.forename: J R
authors.initials: JR
authors.lastname: Olivieri
authors.forename: J
authors.initials: J
authors.lastname: Ruggiero Neto
authors.forename: K
authors.initials: K
authors.lastname: Konno
authors.forename: M S
authors.initials: MS
authors.lastname: Palma
authors.forename: T
authors.initials: T
authors.lastname: Yamane
authors.forename: W F
authors.initials: WF
authors.lastname: de Azevedo
mesh_terms: D015550:Cell Degranulation; D003460:Crystallization; D018360:Crystallography, X-Ray; D008407:Mast Cells / Q000166:cytology*; D011487:Protein Conformation; D014862:Wasp Venoms / Q000737:chemistry*
chemical_list: D014862:Wasp Venoms; C412288:eumenine mastoparan-AF