1-Naphthol as an excited state proton transfer fluorescent probe for erythrocyte membranes.
The microenvironmental dependence of excited state prototropism of 1-naphthol and the corresponding changes in its fluorescence emission is utilized to monitor the acyl chain melting phase transition behavior of liposome membrane made from human erythrocyte lipids. A sharp increase in the ratio of neutral/anionic form fluorescence intensity is noticed at the phase transition temperature (19 degrees C). This provides a convenient method for obtaining phase transition temperature in lipid membranes. The membrane modifying effect of cholesterol on the erythrocyte liposome is successfully sensed by 1-naphthol fluorescence.
Pappayee
N
N
Department of Chemistry, Indian Institute of Technology, Chennai.
Mishra
A K
AK
D002784:Cholesterol / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000737:chemistry* / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008081:Liposomes / Q000737:chemistry* / Q000378:metabolism; D009284:Naphthols / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013696:Temperature
D005456:Fluorescent Dyes; D008081:Liposomes; D009284:Naphthols; C029350:1-naphthol; D002784:Cholesterol