Involvement of multinucleated giant cells synthesizing cathepsin K in calcified tendinitis of the rotator cuff tendons.
OBJECTIVE
Calcified tendinitis of the shoulder joint is a common painful condition. Resorption of the calcium deposits is one of the key events in the pathogenesis of this disease. The aim of this study was to examine whether the multinucleated giant cells that appear in this condition have osteoclast phenotypes.


METHODS
Immunohistochemical and RNA in situ hybridization analysis of cathepsin K, a marker for osteoclasts, was performed in human surgical samples.


RESULTS
The multinucleated cells located near the calcium deposits were positive for cathepsin K protein and mRNA. Reverse transcription-polymerase chain reaction using human cathepsin K-specific oligonucleotide primers confirmed that synthesis of cathepsin K mRNA occurs in the tissues of calcified rotator cuffs.


CONCLUSIONS
The multinucleated giant cells which appear in the resorption area of calcium deposits in calcified tendinitis have the osteoclast phenotype.
Nakase
T
T
Department of Orthopaedic Surgery and. Department of Cell Biology and Anatomy I, Osaka University Medical School, Suita, Japan.
Takeuchi
E
E
Sugamoto
K
K
Kaneko
M
M
Tomita
T
T
Myoui
A
A
Uchiyama
Y
Y
Ochi
T
T
Yoshikawa
H
H
D001175:Arthrography; D002114:Calcinosis / Q000000981:diagnostic imaging / Q000378:metabolism* / Q000473:pathology; D056657:Cathepsin K; D002403:Cathepsins / Q000096:biosynthesis* / Q000235:genetics; D002467:Cell Nucleus / Q000473:pathology; D015726:Giant Cells / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D012333:RNA, Messenger / Q000378:metabolism; D017006:Rotator Cuff / Q000000981:diagnostic imaging / Q000378:metabolism* / Q000473:pathology; D052256:Tendinopathy / Q000000981:diagnostic imaging / Q000378:metabolism* / Q000473:pathology; D013710:Tendons / Q000000981:diagnostic imaging / Q000378:metabolism* / Q000473:pathology
D012333:RNA, Messenger; D002403:Cathepsins; C534130:CTSK protein, human; D056657:Cathepsin K