title: Recruitment of a specific amoeboid myosin I isoform to the plasma membrane in chemotactic Dictyostelium cells.
abstract: The Dictyostelium class I myosins, MyoA, -B, -C, and -D, participate in plasma membrane-based cellular processes such as pseudopod extension and macropinocytosis. Given the existence of a high affinity membrane-binding site in the C-terminal tail domain of these motor proteins and their localized site of action at the cortical membrane-cytoskeleton, it was of interest to determine whether each myosin I was directly associated with the plasma membrane. The membrane association of a myosin I heavy chain kinase that regulates the activity of one of the class I myosins, MyoD was also examined. Cellular fractionation experiments revealed that the majority of the Dicyostelium MyoA, -B, -C and -D heavy chains and the kinase are cytosolic. However, a small, but significant, fraction (appr. 7. -15%) of each myosin I and the kinase was associated with the plasma membrane. The level of plasma membrane-associated MyoB, but neither that of MyoC nor MyoD, increases up to 2-fold in highly motile, streaming cells. These results indicate that Dictyostelium specifically recruits myoB to the plasma membrane during directed cell migration, consistent with its known role in pseudopod formation.
authors.affiliation: Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA.
authors.forename: S
authors.initials: S
authors.lastname: Senda
authors.forename: S F
authors.initials: SF
authors.lastname: Lee
authors.forename: G P
authors.initials: GP
authors.lastname: Côté
authors.forename: M A
authors.initials: MA
authors.lastname: Titus
mesh_terms: D000599:Amino Acids, Diamino; D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D002465:Cell Movement; D002633:Chemotaxis / Q000502:physiology*; D004023:Dictyostelium / Q000502:physiology*; D020409:Molecular Motor Proteins; D009154:Mutation; D009218:Myosins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000599:Amino Acids, Diamino; D020409:Molecular Motor Proteins; D020033:Protein Isoforms; D015800:Protozoan Proteins; D017871:Calcium-Calmodulin-Dependent Protein Kinases; C033142:myosin-heavy-chain kinase; D009218:Myosins