title: Evaluation of the Bactec 960 automated nonradiometric system for isolation of mycobacteria from clinical specimens.
abstract: The Bactec MGIT 960 system (Becton Dickinson, USA), designed for the culture of mycobacteria, was compared with the Bactec 460 instrument and culture on two solid egg-based media using a total of 1024 clinical specimens. Mycobacteria could be identified from 99 (9.7%) specimens, 89 (90%) of which were identified by the Bactec 960 system, 90 (91%) by the Bactec 460 system, and 82 (83%) by culture on the two egg-based media. The Bactec 960 cultures became positive an average of 16.7 days after specimen collection, the Bactec 460 cultures 14.9 days after collection, and the cultures on egg-based media 26.2 days after collection. The Bactec 960 is a compact and highly automated nonradiometric system that may replace the Bactec 460 system.
authors.affiliation: Division des Maladies Infectieuses, Hôpital Cantonal de Genève, Switzerland. peter.rohner@hcuge.ch
authors.forename: P
authors.initials: P
authors.lastname: Rohner
authors.forename: B
authors.initials: B
authors.lastname: Ninet
authors.forename: A M
authors.initials: AM
authors.lastname: Benri
authors.forename: R
authors.initials: R
authors.lastname: Auckenthaler
mesh_terms: D001431:Bacteriological Techniques; D003470:Culture Media; D005470:Fluorometry / Q000295:instrumentation / Q000379:methods; D006801:Humans; D009161:Mycobacterium / Q000145:classification* / Q000302:isolation & purification*; D009164:Mycobacterium Infections / Q000175:diagnosis / Q000382:microbiology*; D010101:Oxygen Consumption
chemical_list: D003470:Culture Media