Determination of chlorophacinone and diphacinone in commercial rodenticides by liquid chromatography-UV detection and liquid chromatography-electrospray ionization mass spectrometry.
This work describes a simple method for the determination and mass-spectrometric confirmation of the indanediones in commercial rodenticides. The sample is sonicated in methanol containing 2% formic acid and analyzed by liquid chromatography-UV detection. Once retention time and UV-Vis spectrum provide tentative identification, mass-spectrometric confirmation is obtained by analyzing a second aliquot by LC and electrospray ionization mass spectrometry (LC-ESI-MS). The extensive fragmentation of the indanedione molecule under MS/MS conditions provides sufficient structural information for positive identification on analyte levels as low as 20 ng on column.
Mesmer
M Z
MZ
United States Food and Drug Administration, Forensic Chemistry Center, Cincinnati, OH 45237-3097, USA.
Flurer
R A
RA
D002853:Chromatography, Liquid / Q000379:methods*; D007189:Indans / Q000032:analysis*; D010630:Phenindione / Q000031:analogs & derivatives* / Q000032:analysis; D012378:Rodenticides / Q000737:chemistry*; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet
D007189:Indans; D012378:Rodenticides; C004662:chlorophacinone; C100262:diphenadione; D010630:Phenindione