Novel goblet cell gene related to IgGFcgammaBP is regulated in adapting gut after small bowel resection.
The loss of functional small bowel surface area leads to a well-described adaptive response in the remnant intestine. To elucidate its molecular regulation, a cohort of cDNAs were cloned using a rat gut resection model and subtractive/differential hybridization cloning techniques. This study reports a novel cDNA termed "ileal remnant repressed" (IRR)-219, which shares 80% nucleotide identity with the 3'end of a human intestinal IgG Fc binding protein (IgGFcgammaBP) and is homologous to human and rat mucins. IRR-219 mRNA is expressed in intestine and colon only. At 48 h after 70% intestinal resection, mRNA levels decreased two- to fivefold in the adaptive small bowel but increased two- to threefold in the colon. Expression of IRR-219 was suppressed in adaptive small bowel as late as 1 wk after resection. IRR-219 expression is also regulated during gut ontogeny. In situ hybridization revealed IRR-219 expression in small intestinal and colonic goblet cells only. Its unique patterns of expression during ontogeny and after small bowel resection suggest distinctive roles in small bowel and colonic adaptation.
Rubin
D C
DC
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA. drubin@im.wustl.edu
Swietlicki
E A
EA
Iordanov
H
H
Fritsch
C
C
Levin
M S
MS
D000222:Adaptation, Physiological / Q000235:genetics*; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics*; D015815:Cell Adhesion Molecules; D003001:Cloning, Molecular; D003106:Colon / Q000502:physiology; D018076:DNA, Complementary; D005786:Gene Expression Regulation / Q000502:physiology; D020397:Goblet Cells / Q000737:chemistry* / Q000502:physiology*; D017403:In Situ Hybridization; D007421:Intestine, Small / Q000166:cytology* / Q000502:physiology / Q000601:surgery*; D008297:Male; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; D018076:DNA, Complementary; C106607:FCGBP protein, human; C416187:IRR-219 protein, rat; D008565:Membrane Proteins; D012333:RNA, Messenger