title: Lymphatic microvessel density in epithelial ovarian cancer: its impact on prognosis.
abstract: BACKGROUND
  The influence of lymphatic microvessel density (MVD) on survival in epithelial ovarian cancer is still unknown, owing to the fact that until recently no reliable immunohistologic markers for lymphatic endothelium were available.
  
  
  METHODS
  By using a polyclonal antibody staining podoplanin, a novel marker for lymphatic endothelium, lymphatic MVD in tissue samples of 90 patients with epithelial ovarian cancer treated by radical surgery and chemotherapy was investigated. Survival analysis was performed using univariate and multivariate analysis. Furthermore, lymphatic MVD was compared to MVD assessed by CD34 immunostaining.
  
  
  RESULTS
  Lymphatic MVD was significantly lower than CD34 MVD (p < 0.0001). There was no significant association between lymphatic MVD and various histological and clinical parameters. Lymphatic MVD had no influence on overall survival and disease free survival (p = 0.4627 and p = 0.4337, respectively; log-rank test).
  
  
  CONCLUSIONS
  The formation of lymphatic vessels has no influence on the progression of epithelial ovarian cancer.
authors.affiliation: Department of Gynecopathology and Cytology, University of Vienna, Austria. peter.birner@akh-wien.ac.at
authors.forename: P
authors.initials: P
authors.lastname: Birner
authors.forename: M
authors.initials: M
authors.lastname: Schindl
authors.forename: A
authors.initials: A
authors.lastname: Obermair
authors.forename: C
authors.initials: C
authors.lastname: Plank
authors.forename: G
authors.initials: G
authors.lastname: Breitenecker
authors.forename: H
authors.initials: H
authors.lastname: Kowalski
authors.forename: G
authors.initials: G
authors.lastname: Oberhuber
mesh_terms: D000367:Age Factors; D000818:Animals; D015415:Biomarkers; D004729:Endothelium, Lymphatic / Q000098:blood supply* / Q000473:pathology; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008562:Membrane Glycoproteins / Q000032:analysis; D008654:Mesothelioma / Q000098:blood supply* / Q000737:chemistry / Q000145:classification / Q000473:pathology; D008833:Microcirculation / Q000473:pathology; D008875:Middle Aged; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000098:blood supply* / Q000737:chemistry / Q000145:classification / Q000473:pathology; D011379:Prognosis; D011817:Rabbits; D012008:Recurrence; D000098853:Podoplanin
chemical_list: D015415:Biomarkers; D008562:Membrane Glycoproteins; C117468:PDPN protein, human; D000098853:Podoplanin