title: Nucleotide sequences of the F, L and G protein genes of two non-A/non-B avian pneumoviruses (APV) reveal a novel APV subgroup.
abstract: Sequence analysis was performed of all or part of the genes encoding the fusion (F), polymerase (L) and attachment (G) proteins of two French non-A/non-B avian pneumovirus (APV) isolates (Fr/85/1 and Fr/85/2). The two isolates shared at least 99.7% nt and 99.0% aa sequence identity. Comparison with the F genes from subgroup A, subgroup B or Colorado APVs revealed nt and aa identities of 70.0-80. 5% and 77.6-97.2%, respectively, with the L gene sharing 76.1% nt and 85.3% aa identity with that of a subgroup A isolate. The Fr/85/1 and Fr/85/2 G genes comprised 1185 nt, encoding a protein of 389 aa. Common features with subgroup A and subgroup B G proteins included an amino-terminal membrane anchor, a high serine and threonine content, conservation of cysteine residues and a single extracellular region of highly conserved sequence proposed to be the functional domain involved in virus attachment to cellular receptors. However, the Fr/85/1 and Fr/85/2 G sequences shared at best 56.6% nt and 31.2% aa identity with subgroup A and B APVs, whereas these isolates share 38% aa identity. Phylogenetic analysis of the F, G and L genes of pneumoviruses suggested that isolates Fr/85/1 and Fr/85/2 belong to a previously unrecognized APV subgroup, tentatively named D. G-based oligonucleotide primers were defined for the specific molecular identification of subgroup D. These are the first G protein sequences of non-A/non-B APVs to be determined.
authors.affiliation: Unité de Virologie, Immunologie et Parasitologie Aviaires et Cunicoles1 and Laboratoire de Biologie Moléculaire2, Agence Française de Sécurité Sanitaire des Aliments (AFSSA), BP 53, 22440 Ploufragan, France.
authors.forename: Marie-Hélène
authors.initials: MH
authors.lastname: Bäyon-Auboyer
authors.affiliation: Unité de Virologie, Immunologie et Parasitologie Aviaires et Cunicoles1 and Laboratoire de Biologie Moléculaire2, Agence Française de Sécurité Sanitaire des Aliments (AFSSA), BP 53, 22440 Ploufragan, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Arnauld
authors.affiliation: Unité de Virologie, Immunologie et Parasitologie Aviaires et Cunicoles1 and Laboratoire de Biologie Moléculaire2, Agence Française de Sécurité Sanitaire des Aliments (AFSSA), BP 53, 22440 Ploufragan, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Toquin
authors.affiliation: Unité de Virologie, Immunologie et Parasitologie Aviaires et Cunicoles1 and Laboratoire de Biologie Moléculaire2, Agence Française de Sécurité Sanitaire des Aliments (AFSSA), BP 53, 22440 Ploufragan, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Eterradossi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001717:Birds / Q000821:virology; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D005814:Genes, Viral; D016679:Genome, Viral; D015707:HN Protein / Q000235:genetics; D008969:Molecular Sequence Data; D018111:Pneumovirus / Q000235:genetics*; D016415:Sequence Alignment; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D015707:HN Protein; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; D014764:Viral Proteins; C116204:attachment protein G; D012321:DNA-Directed RNA Polymerases