Studying organelle physiology with fusion protein-targeted avidin and fluorescent biotin conjugates.
Wu
M M
MM
Department of Molecular and Cell Biology, University of California at Berkeley 94720, USA.
Llopis
J
J
Adams
S R
SR
McCaffery
J M
JM
Teter
K
K
Kulomaa
M S
MS
Machen
T E
TE
Moore
H P
HP
Tsien
R Y
RY
D000818:Animals; D001360:Avidin / Q000737:chemistry* / Q000378:metabolism*; D001710:Biotin / Q000138:chemical synthesis / Q000378:metabolism*; D002460:Cell Line; D004721:Endoplasmic Reticulum / Q000378:metabolism; D019793:Fluorescein / Q000737:chemistry / Q000378:metabolism; D005456:Fluorescent Dyes / Q000494:pharmacology; D006056:Golgi Apparatus / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007425:Intracellular Membranes / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000379:methods*; D008954:Models, Biological; D021381:Protein Transport; D011522:Protons; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000378:metabolism*
D005456:Fluorescent Dyes; D011522:Protons; D011993:Recombinant Fusion Proteins; D001360:Avidin; D001710:Biotin; D019793:Fluorescein