title: Crystallization and preliminary X-ray crystallographic studies of response regulator for cyanobacterial phytochrome, Rcp1.
abstract: The key response-regulator gene of light regulation, rcp1, from Synechocystis sp. has been overexpressed, purified and subsequently crystallized using ammonium sulfate as a precipitant in forms suitable for X-ray crystallographic studies. A native data set was collected to a resolution of 2.5 A at cryogenic temperature. The crystals belong to the hexagonal space group P6(3), with unit-cell parameters a = b = 89.04 (5), c = 60.29 (3) A. The Matthews parameter suggests that Rcp1 crystallizes with two molecules per asymmetric unit.
authors.affiliation: Department of Life Science, Kwangju Institute of Science and Technology, Kwangju 500-712, South Korea.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Im
authors.forename: C M
authors.initials: CM
authors.lastname: Park
authors.forename: J I
authors.initials: JI
authors.lastname: Kim
authors.forename: S S
authors.initials: SS
authors.lastname: Yang
authors.forename: J G
authors.initials: JG
authors.lastname: Kang
authors.forename: S H
authors.initials: SH
authors.lastname: Rho
authors.forename: J I
authors.initials: JI
authors.lastname: Kim
authors.forename: W K
authors.initials: WK
authors.lastname: Song
authors.forename: P S
authors.initials: PS
authors.lastname: Song
authors.forename: S H
authors.initials: SH
authors.lastname: Eom
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D003460:Crystallization; D018360:Crystallography, X-Ray; D000458:Cyanobacteria / Q000737:chemistry*; D008969:Molecular Sequence Data; D011506:Proteins / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D011506:Proteins; C107945:Rcp1 protein, Synechocystis