[Angiotensin II type 2 receptor inhibits type 1 receptor-induced cell proliferation and fibronectin release in mouse fibroblasts].
OBJECTIVE
To investigate the effects of angiotensin II type 2 receptor (AT2) on the cell proliferation and fibronectin release induced by angiotensin II type 1 receptor (AT1).


METHODS
Fibroblats were cultured from embryonic skin of AT2 receptor gene knocked-out mice (AT2-/-) and wild-type mice (AT2+/+) after 16 days of gestation, respectively. Cell proliferation and fibronectin release were examined in the fibroblasts treated with 10(-8) mol/L angiotensin II.


RESULTS
Cell proliferation rate was increased more markedly in the AT2-/- fibroblasts than in the AT2+/+ fibroblasts after treament with angiotensin II (0.462 +/- 0.026 VS 0.389 +/- 0.021, P < 0.01). Also, fibronectin release and its gene expression were significantly enhanced in the AT2-/- fibroblasts as compared with the AT2+/+ fibroblasts when exposed to angiotensin II (34.1 +/- 4.1)% vs (16.2 +/- 2.3)%, P < 0.01).


CONCLUSIONS
The AT2 receptor can inhibit the cell proliferation and fibronectin release induced by the AT1 receptor.
Li
W
W
Department of Nephrology, General Hospital of PLA, Beijing.
Chen
X
X
Ye
Y
Y
D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology; D005353:Fibronectins / Q000096:biosynthesis*; D051379:Mice; D008810:Mice, Inbred C57BL; D044140:Receptor, Angiotensin, Type 1; D044139:Receptor, Angiotensin, Type 2; D011945:Receptors, Angiotensin
D005353:Fibronectins; D044140:Receptor, Angiotensin, Type 1; D044139:Receptor, Angiotensin, Type 2; D011945:Receptors, Angiotensin; D000804:Angiotensin II