title: Scanning electrochemical microscopy. 40. Voltammetric ion-selective micropipet electrodes for probing ion transfer at bilayer lipid membranes.
abstract: Voltammetric ion-selective micropipet electrodes for use in scanning electrochemical microscopy (SECM) for detection of potassium ion were fabricated. These used pulled borosilicate capillaries with tip orifice radii of 0.7-20 microm with silanized inner walls filled with a solution of 10 mM valinomycin and 10 mM ETH 500 in dichloroethane. The electrodes were characterized by determining the steady-state tip current for K+ concentrations of 0.05-0.3 mM. The tips were used in the SECM feedback and generation-collection modes to study K+ transfer through gramicidin channels in a horizontal bilayer lipid membrane (glycerol monooleate).
authors.affiliation: Department of Chemistry and Biochemistry, The University of Texas at Austin, 78712-1167, USA.
authors.forename: S
authors.initials: S
authors.lastname: Amemiya
authors.forename: A J
authors.initials: AJ
authors.lastname: Bard
mesh_terms: D004566:Electrodes; D007477:Ions; D008051:Lipid Bilayers; D008855:Microscopy, Electron, Scanning / Q000379:methods*
chemical_list: D007477:Ions; D008051:Lipid Bilayers