Mouse Tspan-5, a member of the tetraspanin superfamily, is highly expressed in brain cortical structures.
Using a subtractive hybridization method for the identification of genes related to the development of the murine cerebral cortex, we cloned a mouse homologue of a human tetraspanin family member, Tspan-5. We have isolated a 3.1 Kb cDNA fragment containing the entire coding region. Analysis of the cDNA nucleotide sequence revealed that mouse Tspan-5 shares 98% amino acid sequence identity with its human homologue. The predicted length of the mouse protein is 268 amino acids, with four putative hydrophobic domains with N- and C-intracellular tails, and two extracellular domains. Northern blot analysis of adult mouse tissues showed a single transcript, which is preferentially expressed in the brain. In situ hybridization showed prominent expression of Tspan-5 in the neocortex, the hippocampus, amygdala and in Purkinje cells in the cerebellum. The pattern of expression of Tspan-5 in the mouse brain suggests a role for the tetraspanins in the maintenance of adult brain function.
García-Frigola
C
C
Department of Cell Biology, Faculty of Biology, and Neurosciences Research Center (CERN), University of Barcelona, Spain.
Burgaya
F
F
Calbet
M
M
de Lecea
L
L
Soriano
E
E
D000818:Animals; D001483:Base Sequence / Q000502:physiology; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism; D003001:Cloning, Molecular; D005333:Fetus; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D017434:Protein Structure, Tertiary / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic / Q000502:physiology
D008565:Membrane Proteins; D012333:RNA, Messenger; C111029:TM4SF5 protein, human