title: Integrated nuclear fluorescence and expression of hormone-binding sites in malignant pleural effusions.
abstract: OBJECTIVE
  To investigate potential disease- and prognosis-associated nuclear and cellular features from cell properties in a prospective study on malignant pleural effusions.
  
  
  METHODS
  Integrated nuclear fluorescence and the expression of binding capacities of carrier-immobilized estradiol, progesterone and testosterone; and of labeled sarcolectin; and the presence of calcyclin were measured in 50 cases with proven malignant pleural effusions (10 mesotheliomas, 40 metastasizing tumors). A double fluorescence technique using the fluorochrome DAPI and a Texas Red-based avidin-biotin detection system were applied. Detailed clinical data, including the follow-up for up to 40 months, were included.
  
  
  RESULTS
  Pleural effusions in all patients with mesotheliomas occurred prior to (9/10) or at the time of histologic confirmation. Mesotheliomas had the highest tumor cell fraction (12.4%) in S phase and breast carcinomas the lowest (10.7%). More than 80% of malignant cells expressed binding capacities for the applied probes. A statistically significant correlation was noted between the S-phase-related tumor cell fraction and the expression of progesterone receptors. Survival was associated with tumor origin, treatment by pleurodesis, and certain cytometric and histochemical features.
  
  
  CONCLUSIONS
  The immunofluorescence double-staining technique can be applied successfully in malignant effusions to combine DNA measurements with those of immunohistochemical and ligand histochemical reactivity.
authors.affiliation: Department of Pathology, Thoraxklinik, Heidelberg, Germany. klkayser@lung.de
authors.forename: K
authors.initials: K
authors.lastname: Kayser
authors.forename: C
authors.initials: C
authors.lastname: Märkle
authors.forename: C
authors.initials: C
authors.lastname: Kugler
authors.forename: S
authors.initials: S
authors.lastname: Andre
authors.forename: M P
authors.initials: MP
authors.lastname: Schüring
authors.forename: F Y
authors.initials: FY
authors.lastname: Zeng
authors.forename: H J
authors.initials: HJ
authors.lastname: Gabius
mesh_terms: D000368:Aged; D001665:Binding Sites; D002467:Cell Nucleus / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007211:Indoles / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D016066:Pleural Effusion, Malignant / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D011374:Progesterone / Q000378:metabolism*; D011379:Prognosis; D011446:Prospective Studies; D016019:Survival Analysis; D013739:Testosterone / Q000378:metabolism*; D014966:Xanthenes / Q000378:metabolism
chemical_list: D007211:Indoles; D014966:Xanthenes; D013739:Testosterone; C007293:DAPI; D011374:Progesterone; D004958:Estradiol; C034657:Texas red; D004247:DNA