Method for linking a synthesized protein to its mRNA-DNA complex.
A nascent protein remains in a complex with its ribosome and mRNA if the stop codon is deleted from the mRNA. In the same manner, mRNA forms a stable complex with DNA if the transcription termination is blocked. In principle, if both mRNA translation and DNA transcription termination are prevented, the protein should stay in a complex with its mRNA and DNA. A method is designed to test these possibilities. Using an immobilized luciferase gene sequence, a functional luciferase protein is produced that remains associated through its mRNA with its DNA, confirming the feasibility of the proposed scheme. It has potential application for in vitro synthesis of proteins and protein micro-arrays.
Liu
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S
NEC Research Institute, Princeton, NJ 08540, USA. shumo@research.nj.nec.com
Shivashankar
G V
GV
Sano
T
T
Libchaber
A
A
D004247:DNA / Q000378:metabolism*; D011691:Puromycin / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism*; D014158:Transcription, Genetic
D012333:RNA, Messenger; D011691:Puromycin; D004247:DNA