Expression and electrophysiological function of actin in chick cerebellar neurons.
Among several monoclonal antibodies obtained by immunizing Balb/c mice with cerebellar synaptic membrane fractions from E20 chick embryos, the antibody, named M35, suppressed Ca-spikes in immature cultured chick cerebellar neurons. M35 immunoprecipitated 43 kDa protein from a 125I-labeled embryonic crude cerebellar membrane fraction. Immunohistochemically, the M35 antigen was expressed most intensively in Purkinje cells, but its expression was limited to highly motile structures at developmental neuronal remodeling. Electrophysiologically, M35 facilitated current responses to AMPA and inhibited the responses to GABA in cultured cerebellar Purkinje neurons. The several peptides derived from the affinity-purified 43 kDa protein were found to have homologous amino acid sequences to non-muscle actins. These results suggest that the antigen recognized by M35 may play an essential role probably as membrane ion channels modulating synaptic functions in not only the development and growth but also the neuronal activity of chick cerebellar Purkinje cells.
Tandai-Hiruma
M
M
Department of Physiology, National Defense Medical College, Tokorozawa, Saitama, Japan. meguth@ndmc.ac.jp
Mori-Okamoto
J
J
Kotani
M
M
Miura
K
K
Takishima
K
K
Nishida
Y
Y
D000199:Actins / Q000737:chemistry / Q000276:immunology / Q000502:physiology*; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D002118:Calcium / Q000494:pharmacology / Q000502:physiology; D002462:Cell Membrane / Q000276:immunology / Q000502:physiology; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000196:embryology / Q000502:physiology*; D002642:Chick Embryo; D005260:Female; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D011689:Purkinje Cells / Q000166:cytology / Q000502:physiology*; D019789:Tetraethylammonium / Q000494:pharmacology; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / Q000494:pharmacology
D000199:Actins; D000911:Antibodies, Monoclonal; D019789:Tetraethylammonium; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; D002118:Calcium