Detection of damage-recognition proteins from human lymphocytes.
Proteins recognizing and binding to damaged DNA (DDB-proteins) were analyzed in human lymphocytes obtained from healthy donors. Using an electrophoretic mobility shift assay several complexes between nuclear extract proteins and damaged DNA were detected: a complex specific for DNA damaged by N-acetoxy-N-acetylaminofluorene, another complex specific for UV-irradiated DNA, and two complexes specific for DNA damaged by cis-dichlorodiammine platinum. All the detected complexes differed in electrophoretic mobility and possibly contained different proteins. Complexes specific for free DNA ends were also detected in protein extracts from lymphocytes.
Lanuszewska
J
J
Department of Experimental and Clinical Radiobiology, Center of Oncology, Gliwice, Poland.
Cudak
A
A
Rzeszowska-Wolny
J
J
Widłak
P
P
D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide / Q000633:toxicity; D000099:Acetoxyacetylaminofluorene / Q000633:toxicity; D000328:Adult; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D002945:Cisplatin / Q000633:toxicity; D003593:Cytoplasm / Q000378:metabolism; D004249:DNA Damage; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D015345:Oligonucleotide Probes; D014466:Ultraviolet Rays
D004268:DNA-Binding Proteins; D015345:Oligonucleotide Probes; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; D000099:Acetoxyacetylaminofluorene; D002945:Cisplatin