Neutral red retention by earthworm coelomocytes: a biomarker of cadmium contamination in soil.
The earthworm Metaphire posthuma were used as a model to assess the toxic potential of cadmium incorporated into the soil by environmental or human activities. The retention period of neutral red in the lysosomes of the coelomocytes was used as a biomarker. The viability of harvested coelomocytes by a non-invasive extrusion protocol was 93% with no alteration by the dye during experimentation. The control cells retained dye for 119 and 121 min in normal soil and KCl, respectively, whereas a linear decline in the retention time in the treated earthworm coelomocytes was observed. This illustrated that the presence of cadmium caused damage to the lysosomes of the coelomocytes.
Gupta
S K
SK
Cell Biology Section, Industrial Toxicology Research Centre, Lucknow, India. ntox@itrc.sirnctd.ernet.in
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002104:Cadmium / Q000633:toxicity*; D004396:Coloring Agents / Q000493:pharmacokinetics; D004064:Digestive System / Q000166:cytology* / Q000187:drug effects; D004784:Environmental Monitoring / Q000379:methods*; D009499:Neutral Red / Q000493:pharmacokinetics; D009835:Oligochaeta / Q000737:chemistry* / Q000502:physiology
D015415:Biomarkers; D004396:Coloring Agents; D002104:Cadmium; D009499:Neutral Red