The quantitative characteristics of efficiency of ballistic transfection of chimeric antibody genes.
A quantitative approach was applied to the study of in vivo expression of foreign genes introduced into mice by ballistic transfection. Because in some cases one must take into account both the level of synthesized protein and that of antibodies to it, we derived the equation which allows to calculate the exact quantity of both proteins. This formula was applied to in vivo expression of a chimeric (human/mice) immunoglobulin E gene. The immunochemical analysis using this equation showed that the Ig concentration succeeded 4, 6, 12 IU/ml and undetectable level, respectively, upon transfection in mouse liver, spleen, foot pad and ear cartilage.
Rodin
D V
DV
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov Street 32, 117984, Moscow, Russia. rodin@imb.ac.ru
Kolesnikov
V A
VA
Zelenina
I A
IA
Zelenin
A V
AV
Deyev
S M
SM
D000818:Animals; D019470:Biolistics; D002356:Cartilage / Q000378:metabolism; D004274:DNA, Recombinant / Q000008:administration & dosage*; D004423:Ear; D005528:Foot; D005803:Genes, Immunoglobulin; D005813:Genes, Synthetic; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis / Q000097:blood / Q000235:genetics*; D008099:Liver / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009928:Organ Specificity; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000097:blood / Q000235:genetics; D013154:Spleen / Q000378:metabolism; D014162:Transfection / Q000295:instrumentation / Q000379:methods*
D004274:DNA, Recombinant; D011993:Recombinant Fusion Proteins; D007073:Immunoglobulin E