title: Induction of Ro/SSA antigen expression on keratinocyte cell membrane by heat shock and phorbol 12-myristate 13-acetate as well as estradiol and ultraviolet B.
abstract: Skin is one of the main target organs in lupus erythematosus and in some circumstances, skin lesions precede systemic manifestations. Previous studies have demonstrated that Ro/SSA antigen antibody might be involved in the pathogenesis of lupus erythematosus. The present study was performed to investigate the factors regulating expression of Ro/SSA antigens on the cell surface of keratinocytes. Cultured normal human keratinocytes were treated with 50-200 mJ/cm(2) of ultraviolet B (UVB) irradiation, 10(-9) to 10(-5) mol/l of 17beta-estradiol, 5-10 microg/l of phorbol 12-myristate 13-acetate (PMA), and 42 and 45 degrees C heat shock, respectively. The Ro/SSA antigen expressions were determined by indirect immunofluorescence. The results showed that keratinocytes receiving UVB irradiation expressed Ro/SSA antigen on cell membranes in a dose-dependent fashion. 17beta-estradiol treatment also induced Ro/SSA antigen expression dose-dependently. Keratinocyte expression of Ro/SSA antigens was also induced by heat shock stimulation. The 45 degrees C heat shock showed a stronger effect than 42 degrees C heat shock. Keratinocytes incubated for 24 h after heat shock had more antigen-expressing cells than those incubated for 6 h after heat shock. PMA at 5 and 10 microg/l also strongly induced Ro/SSA antigen expression. These results suggest that Ro/SSA antigen expression can be regulated by many factors and that protein kinase C signal transduction pathway might be involved in this process.
authors.affiliation: Department of Dermatology, People's Hospital, Beijing Medical University, 100044, Beijing, PR China. derma-bmu@yahoo.com
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: Z
authors.initials: Z
authors.lastname: Xu
authors.forename: J
authors.initials: J
authors.lastname: Jin
authors.forename: T
authors.initials: T
authors.lastname: Zhu
authors.forename: S
authors.initials: S
authors.lastname: Ma
mesh_terms: D001324:Autoantigens / Q000378:metabolism*; D002462:Cell Membrane / Q000276:immunology; D002478:Cells, Cultured; D004958:Estradiol / Q000494:pharmacology*; D006358:Hot Temperature; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000276:immunology* / Q000528:radiation effects; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins / Q000378:metabolism*; D012769:Shock / Q000276:immunology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014466:Ultraviolet Rays; D000099207:SS-A Antigen
chemical_list: D001324:Autoantigens; D004958:Estradiol; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D013755:Tetradecanoylphorbol Acetate; D000099207:SS-A Antigen; C116006:RO60 protein, human