Human root dentin: structural anisotropy and Vickers microhardness isotropy.
The demanding mechanical functions and the variable structure of dentin make it an invaluable material for studying the structure-mechanical function relations of a mineralized collagen-containing tissue. The mineralized collagen fibril axes in human root dentin are mainly located on the incremental plane. Within this plane there is a preferred orientation in the general root-crown direction. The apatite crystals are aligned in three dimensions within an individual collagen fibril, but this orientation does not necessarily extend to the neighboring fibrils. Crystals are also present as aggregates without any preferred orientation. The structure is therefore clearly anisotropic with respect to the collagen fibril orientation, but less so with respect to overall crystal orientation. Vickers microhardness measurements of the root dentin are essentially the same on the three orthogonal planes with respect to the incremental plane. Knoop microhardness measurements are also the same on all three orthogonal planes when the major diagonal is aligned perpendicular to the collagen fibril axis preferred orientation direction. In-plane variations of up to 20% are observed in the orthogonal direction. The material is thus isotropic in the three main directions with respect to Vickers microhardness, but anisotropic in structure. This paradoxical situation is attributed mainly to the variable modes of crystal organization.
Wang
R
R
Department of Structural Biology, Weizmann Institute of Science, Rehovot, Israel.
Weiner
S
S
D000293:Adolescent; D000328:Adult; D003094:Collagen / Q000648:ultrastructure; D018360:Crystallography, X-Ray; D003804:Dentin / Q000648:ultrastructure*; D004577:Electron Probe Microanalysis; D006801:Humans; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure
D003094:Collagen