title: Automated image analysis for monitoring oxidative burst in macrophages.
abstract: OBJECTIVE
  To evaluate oxidative bursts induced by phorbol myristate acetate in phagocytes at the single-cell level by automated image analysis.
  
  
  METHODS
  The generation of reactive oxygen species was quantitatively expressed by means of histograms displaying the percentage of cells corresponding to each of the total optical densities measured.
  
  
  RESULTS
  Macrophage subpopulations were quantitatively defined. This method allows detailed analysis of the amount of formazan per cell and the sites of deposition of blue precipitate in each cell.
  
  
  CONCLUSIONS
  Image analysis is a reliable quantitative, single-cell assay for studying various cellular characteristics associated with macrophage functions.
authors.affiliation: Department of Radiobiology, National Atomic Energy Commission, Buenos Aires, Argentina. beamolin@cnea.gov.ar
authors.forename: B L
authors.initials: BL
authors.lastname: Molinari
authors.forename: D R
authors.initials: DR
authors.lastname: Tasat
authors.forename: M L
authors.initials: ML
authors.lastname: Fernández
authors.forename: H A
authors.initials: HA
authors.lastname: Durán
authors.forename: J
authors.initials: J
authors.lastname: Curiale
authors.forename: A
authors.initials: A
authors.lastname: Stoliar
authors.forename: R L
authors.initials: RL
authors.lastname: Cabrini
mesh_terms: D000818:Animals; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D016676:Macrophages, Alveolar / Q000187:drug effects / Q000378:metabolism*; D010586:Phagocytes / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species; D016897:Respiratory Burst / Q000502:physiology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D005456:Fluorescent Dyes; D017382:Reactive Oxygen Species; D013755:Tetradecanoylphorbol Acetate