Changes of expression level of the differentiation markers in papillary thyroid carcinoma under thyrotropin suppression therapy in vivo immunohistochemical detection of thyroglobulin, thyroid peroxidase, and thyrotropin receptor.
OBJECTIVE
Differences in the expression levels of Thyroglobulin (Tg), Thyroid peroxidase (TPO) and thyrotropin receptor (TSH-R) in primary and recurrent specimens under a suppressive serum TSH condition were elucidated in 26 papillary carcinoma patients.


METHODS
Immunohistochemical detection was performed by use of each monoclonal antibody against Tg, TPO, and TSH-R. The staining concentrations of the three markers in each specimen were measured for comparison.


RESULTS
The mean staining concentrations of Tg, TPO, and TSH-R in the entire primary tumor were 103.92, 104.6 and 89.25, respectively. Five cases showed stronger expression of all the differentiation markers and eight cases showed weaker expression of all these markers in recurrent tissue than in primary tumors. The weaker expression of TSH-R at the recurrent site as compared with that at the primary site significantly demonstrated the shortness of the disease free interval or overall survival. There were significant differences between the death due to cancer and the weaker expression of TSH-R in the recurrent tumor as compared with that in the primary tumor.


CONCLUSIONS
Under the TSH suppressive condition, the markers were not expressed uniformly among recurrent tumors. Even under that state, however, low expression of TSH-R in the recurrent tissue was strongly related to a poorer outcome in the patients.
Tanaka
K
K
Department of Breast and Thyroid Surgery, Kawasaki Medical School, Kurashiki, Japan.
Sonoo
H
H
Yamamoto
Y
Y
Udagawa
K
K
Kunisue
H
H
Arime
I
I
Yamamoto
S
S
Kurebayashi
J
J
Shimozuma
K
K
D000328:Adult; D000368:Aged; D002291:Carcinoma, Papillary / Q000737:chemistry* / Q000201:enzymology / Q000601:surgery*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D007453:Iodide Peroxidase / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000737:chemistry / Q000601:surgery; D011989:Receptors, Thyrotropin / Q000032:analysis*; D013954:Thyroglobulin / Q000032:analysis*; D013964:Thyroid Neoplasms / Q000737:chemistry* / Q000201:enzymology / Q000601:surgery*; D013972:Thyrotropin / Q000097:blood*
D011989:Receptors, Thyrotropin; D013972:Thyrotropin; D013954:Thyroglobulin; D007453:Iodide Peroxidase