title: Regulation of Hoxb3 expression in the hindbrain and pharyngeal arches by rae28, a member of the mammalian Polycomb group of genes.
abstract: During animal development, Hox genes are expressed in characteristic, spatially restricted patterns and specify regional identities along the anterior-posterior (A-P) axis. Polycomb group (PcG) proteins in Drosophila repress Hox expression and maintain the expression patterns during development. Mice deficient for homologues of the Drosophila PcG genes, such as M33, bmi1, mel18, rae28 and eed, show altered Hox expression patterns. In this study, we examined the time course of Hoxb3 expression during late gastrulation and early segmentation of rae28-deficient mice. Hoxb3 was expressed ectopically in pharyngeal arch and hindbrain from embryonic day (E) 9.5 and 10.5, respectively. The anterior boundary of ectopic expression in the hindbrain extended gradually in the rostral direction as development proceeded from E10.5 to E12.5. Expression of kreisler and Krox20, which function as positive regulators of Hoxb3 expression, was not affected in rae28-deficient embryos. Analysis of a neural crest marker, p75, in rae28-deficient mice revealed that the neural crest cells begin to ectopically express Hoxb3 after leaving the hindbrain. Our results suggest that rae28 is not required for the establishment but maintenance of Hoxb3 expression.
authors.affiliation: Department of Medical Genetics, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, 565-0871, Osaka, Japan. dtomo@bcm.tmc.edu
authors.forename: D
authors.initials: D
authors.lastname: Tomotsune
authors.forename: M
authors.initials: M
authors.lastname: Shirai
authors.forename: Y
authors.initials: Y
authors.lastname: Takihara
authors.forename: K
authors.initials: K
authors.lastname: Shimada
mesh_terms: D000818:Animals; D001483:Base Sequence; D001934:Branchial Region / Q000196:embryology*; D002352:Carrier Proteins; D017931:DNA Primers / Q000235:genetics; D004330:Drosophila / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D018398:Homeodomain Proteins / Q000235:genetics*; D017403:In Situ Hybridization; D051379:Mice; D018345:Mice, Knockout; D009432:Neural Crest / Q000378:metabolism; D063150:Polycomb Repressive Complex 1; D012249:Rhombencephalon / Q000196:embryology*; D015534:Trans-Activators / Q000235:genetics; D029867:Xenopus Proteins
chemical_list: D002352:Carrier Proteins; D017931:DNA Primers; D018398:Homeodomain Proteins; C078105:Hox 2.7 protein, Xenopus; C103827:Phc1 protein, mouse; D015534:Trans-Activators; D029867:Xenopus Proteins; D063150:Polycomb Repressive Complex 1