Cynomolgus monkey liver aldehyde oxidase: extremely high oxidase activity and an attempt at purification.
Aldehyde oxidase (EC 1.2.3.1) in monkey (Macaca fascicularis) liver was characterized. Liver cytosol exhibited extremely high benzaldehyde and phthalazine oxidase activities based on aldehyde oxidase, compared with those of rabbits, rats, mice and guinea pigs. Monkey liver aldehyde oxidase showed broad substrate specificity distinct from that of the enzyme from other mammals. Purified aldehyde oxidase from monkey liver cytosol showed two major bands and two minor bands in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). These bands were also observed in Western blotting analysis using anti-rat aldehyde oxidase. The molecular mass of the enzyme was estimated to be 130-151 kDa by SDS-PAGE, and to be about 285 kDa by HPLC gel filtration. The results suggest that isoforms of aldehyde oxidase exist in monkey livers.
Sugihara
K
K
Institute of Pharmaceutical Science, Hiroshima University School of Medicine, Japan.
Katsuma
Y
Y
Kitamura
S
S
Ohta
S
S
Fujitani
M
M
Shintani
H
H
D042931:Aldehyde Oxidase; D000445:Aldehyde Oxidoreductases / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D001547:Benzaldehydes / Q000378:metabolism; D015153:Blotting, Western; D002851:Chromatography, High Pressure Liquid; D003600:Cytosol / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D006168:Guinea Pigs; D007527:Isoenzymes; D008099:Liver / Q000201:enzymology*; D008252:Macaca fascicularis / Q000502:physiology*; D008297:Male; D051379:Mice; D008970:Molecular Weight; D009536:Niacinamide / Q000031:analogs & derivatives* / Q000378:metabolism; D010793:Phthalazines / Q000378:metabolism; D011743:Pyrimidines / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D013045:Species Specificity; D013379:Substrate Specificity
D001547:Benzaldehydes; D007527:Isoenzymes; D010793:Phthalazines; D011743:Pyrimidines; D009536:Niacinamide; C041345:2-hydroxypyrimidine; C043388:phthalazine; D000445:Aldehyde Oxidoreductases; D042931:Aldehyde Oxidase; C032175:benzaldehyde; C008472:N-methylnicotinamide