title: [Expression and characterization of human vascular endothelial growth factor receptor Flt-1 extracellular domain in Pichia pastoris].
abstract: By inserting VEGF receptor Flt-1(1-3 loop) cDNA into Pichia pastoris expression vector pPIC9K containing AOX1 promotor and the sequences of alpha secreting signal peptides, the expression plasmid pPIC9K/Flt-1(1-3) was constructed and transformed into GS115. The multi-copy insert transformants were selected and cultivated in flasks. After 4 days of 1% methanol induction, the expressed Flt-1(1-3) accumulated up to 30% of total proteins in supernatant. The expressed Flt-1(1-3) was further proved with good antigenicity and high specificity by ELISA and Western blot. They can bind to VEGF and inhibit HUVEC proliferation stimulated by VEGF.
authors.affiliation: Institute of Virology, Chinese Academy of Preventive Medicine, Beijing.
authors.forename: L
authors.initials: L
authors.lastname: Ma
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Zhang
authors.forename: X M
authors.initials: XM
authors.lastname: Zhou
authors.forename: G F
authors.initials: GF
authors.lastname: Zeng
authors.forename: A J
authors.initials: AJ
authors.lastname: Chen
authors.forename: L H
authors.initials: LH
authors.lastname: Yao
authors.forename: X N
authors.initials: XN
authors.lastname: Wang
mesh_terms: D006801:Humans; D010843:Pichia / Q000235:genetics*; D010957:Plasmids; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics; D020794:Receptor Protein-Tyrosine Kinases / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis*; D040281:Vascular Endothelial Growth Factor Receptor-1
chemical_list: D011518:Proto-Oncogene Proteins; D011994:Recombinant Proteins; D020794:Receptor Protein-Tyrosine Kinases; D040281:Vascular Endothelial Growth Factor Receptor-1