[High efficient intergeneric chromosomal translocations between wheat (Triticum aestivum L.) and Dasypyrum villosum arising from tissue culture and irradiation].
Intergeneric chromosomal translocations were discernable both in callus cells and in regenerants arising from crosses between Triticum aestivum and T. durum-Dasypyrum villosum amphiploid c.v. TH1 and TH1W by means of fluorescence in situ hybridization. There were not only reciprocal translocations, but small fragment translocations. The results proved again the feasibility of creating intergeneric translocations via tissue culture. Irradiation facilitated numerical and structural chromosome changes in callus cells. The frequency of translocations was as high as 7.4 percent in irradiated callus cells. Callus age had an important impact on numerical and structural chromosome abnormalities. During a given time of culture, the frequency of unchanged cells was declined, while those cells with chromosome losses were inclined. The duration of culture had not significant effects on cells with chromosome gains. As structural chromosome changes is concerned, the duration of culture predominantly increased the frequency of cells with telocentric chromosomes. The chromosomal changes occurred at the initiation period of tissue culture. A number of cells which were doubled their chromosome numbers (2n = 84) were observed at a certain frequency in the period of tissue culture. These cells, however, disappeared in the successive culture.
Li
H J
HJ
Institute of Botany, Chinese Academy of Sciences, Beijing, China.
Guo
B H
BH
Zhang
Y M
YM
Li
Y W
YW
Du
L Q
LQ
Li
Y X
YX
Jia
X
X
Zhu
Z Q
ZQ
D003433:Crosses, Genetic; D046508:Culture Techniques; D002523:Edible Grain / Q000235:genetics*; D014178:Translocation, Genetic; D014908:Triticum / Q000235:genetics* / Q000528:radiation effects