[Phenotypic changes in transgenic tobacco plants with an antisense form of the hmg1 gene].
Tobacco plants were genetically transformed with the Arabidopsis thaliana heterologous hmg1 gene encoding 3-hydroxy-3-methylglutaryl-CoA reductase, a key enzyme involved in the metabolism of terpenoid compounds. The hmg1 gene was inserted under the control of the 35S RNA double promoter from the cauliflower mosaic virus (CaMV 35S) both in direct and reverse orientation relative to the promoter. DNA analysis by polymerase chain reaction (PCR) and Southern blotting confirmed the transgenic nature of the tobacco plants obtained. DNA-RNA hybridization revealed expression of the hmg1 gene in these tobacco plants. The plants transformed with the antisense copy of the hmg1 gene differed from the control plants in delayed development and in flower color and shape.
Poroĭko
V A
VA
Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, Moscow, Russia.
Rukavtsova
E B
EB
Orlova
I V
IV
Bur'ianov
Ia I
IaI
D016375:Antisense Elements (Genetics); D017360:Arabidopsis / Q000235:genetics; D001483:Base Sequence; D015139:Blotting, Southern; D017796:Caulimovirus / Q000235:genetics; D017931:DNA Primers; D018506:Gene Expression Regulation, Plant; D006903:Hydroxymethylglutaryl CoA Reductases / Q000235:genetics*; D025782:Hydroxymethylglutaryl-CoA-Reductases, NADP-dependent; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D030821:Plants, Genetically Modified / Q000235:genetics*; D010947:Plants, Toxic; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D014026:Nicotiana / Q000235:genetics*; D014170:Transformation, Genetic
D016375:Antisense Elements (Genetics); D017931:DNA Primers; D006903:Hydroxymethylglutaryl CoA Reductases; D025782:Hydroxymethylglutaryl-CoA-Reductases, NADP-dependent