title: [Content determination of baicalin and baicalein in shuanghuanglian powdery injection by the method of HPLC-ECD].
abstract: The contents of baicalin and baicalein in Shuanghuanglian Powdery Injection have been determined by the method of HPLC-ECD under following conditions: column-ERC-ODS-1161, 6 mm x 100 mm; temperature-55 degrees C; mobile phase-0.1 mol/L KH2PO4, acetic acid and tetrahydrofuran (1000:100:190); flow rate-0.8 ml/min; detector voltage-+650 mV. The result shows the limit of detection for baicalin and baicalein to be 25 ng and 50 ng respectively, with an average recovery of 100.1% and 99.7% respectively. Highly reproducible, rapid, simple, convenient and accurate, this method is good for the content determination of baicalin and baicalein in Chinese medicinal herbs and preparations.
authors.affiliation: Heilongjiang Provincial Hospital, Harbin.
authors.forename: X
authors.initials: X
authors.lastname: He
authors.forename: C
authors.initials: C
authors.lastname: Shi
authors.forename: Y
authors.initials: Y
authors.lastname: Feng
authors.forename: N
authors.initials: N
authors.lastname: Kohri
authors.forename: K
authors.initials: K
authors.lastname: Iseki
authors.forename: S
authors.initials: S
authors.lastname: Miyazaki
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D044950:Flavanones; D005419:Flavonoids / Q000032:analysis*; D007267:Injections
chemical_list: D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005419:Flavonoids; C038044:baicalin; C006680:baicalein