Flow cytometry for the detection of enterohaemorrhagic Escherichia coli O157:H7 with latex beads sensitized with specific antibody.
To detect low concentrations of enterohaemorrhagic Escherichia coli O157:H7 rapidly, flow cytometry (FCM) was carried out with specific IgG-sensitized latex beads (IgG-Lx). It was found that test samples for FCM can be prepared for much shorter periods by culturing E. coli O157:H7 in trypto-soya broth at 42 degrees C and by treatment with 0.5% formalin at 37 degrees C. FCM with IgG-Lx performed with E. coli O157:H7 prepared by such a procedure revealed that the lowest number of E. coli O157:H7 prepared in pure culture detected by FCM was 10(3)/ml. Because similar findings have already been reported by FCM with immunomagnetic beads, FCM with IgG-Lx is also suggested to be a valuable technique to detect low numbers of E. coli O157:H7 rapidly in food stuffs.
Kusunoki
H
H
Department of Veterinary Science, College of Agriculture, Osaka Prefecture University, Japan.
Bari
M L
ML
Kita
T
T
Sugii
S
S
Uemura
T
T
D000818:Animals; D000907:Antibodies, Bacterial; D004927:Escherichia coli Infections / Q000382:microbiology / Q000517:prevention & control; D019453:Escherichia coli O157 / Q000302:isolation & purification*; D005434:Flow Cytometry / Q000662:veterinary*; D005516:Food Microbiology; D005759:Gastroenteritis / Q000382:microbiology / Q000517:prevention & control; D008863:Microspheres; D011237:Predictive Value of Tests
D000907:Antibodies, Bacterial