Growing and regenerating axons in the visual system of teleosts are recognized with the antibody RT97.
We have analyzed the immunolabeling with the antibody RT97, a good marker for ganglion cell axons in several species, in the normal and regenerating visual pathways of teleosts. We have demonstrated that RT97 antibody recognizes several proteins in the tench visual system tissues (105, 115, 160, 200, 325 and 335 kDa approximately). By using immunoprecipitation and Western blot we have found that after crushing the optic nerve the immunoreactivity to anti RT97 increased markedly in the optic nerve. In immunohistochemical analysis we also found a different pattern of labeling in normal and regenerating visual pathways. In normal tench RT97 is a good marker for the horizontal cells in the retina, for growing ganglion cell axons which run along the optic nerve from the retina to the optic tectum and of the axon terminals in the stratum opticum and stratum fibrosum and griseum superficiale in the optic tectum. After optic nerve crush, no immunohistochemistry modifications were observed in the retina. However, in accordance with Western blot experiments, in the optic nerve intensely stained groups of regenerating axons appeared progressively throughout the optic nerve as far as the optic tectum. We conclude that the antibody RT97 is an excellent marker of growing and regenerating axons of the optic nerve of fish.
Velasco
A
A
Instituto de Neurociencias de Castilla y León, Departamento de Biología Celular y Patología, Universidad de Salamanca, E-37007, Salamanca, Spain.
Bragado
M J
MJ
Jimeno
D
D
Caminos
E
E
Lillo
C
C
Aijón
J
J
Lara
J M
JM
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D001369:Axons / Q000502:physiology*; D015153:Blotting, Western; D005399:Fishes / Q000502:physiology*; D007150:Immunohistochemistry; D008869:Microtubule-Associated Proteins / Q000276:immunology; D008970:Molecular Weight; D009416:Nerve Regeneration / Q000502:physiology*; D016900:Neurofilament Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D009900:Optic Nerve / Q000378:metabolism; D012160:Retina / Q000378:metabolism / Q000502:physiology; D013477:Superior Colliculi / Q000378:metabolism; D014795:Visual Pathways / Q000502:physiology*
D000906:Antibodies; D000911:Antibodies, Monoclonal; D008869:Microtubule-Associated Proteins; D016900:Neurofilament Proteins; C085284:microtubule-associated protein 1B