title: Mesenchymal precursor cells in the blood of normal individuals.
abstract: STATEMENT OF FINDINGS: Mesenchymal precursor cells found in the blood (BMPCs) of normal persons adhere to plastic and glass and proliferate logarithmically in DMEM-20% fetal calf serum (FCS) without growth factors. They form cells with fibroblast-like and stromal morphology, which is not affected by eliminating CD34, CD3, or CD14 cells. Osteogenic supplements (dexamethasone, ascorbic acid, and beta-glycerophosphate) added to the culture inhibited fibroblast formation, and BMPCs assumed the cuboidal shape of osteoblasts. After 5 days in supplemented medium, the elutriated cells displayed alkaline phosphatase (AP), and the addition of bone morphogenetic protein (BMP)2 (1 ng) doubled AP production (P < 0.04). Two weeks later, 30% of the cells were very large and reacted with anti-osteocalcin antibody. The same cultures also contained sudanophlic adipocytes and multinucleated giant cells that stained for tartrate-resistant acid phosphatase (TRAP) and vitronectin receptors. Cultured BMPCs immunostain with antibodies to vimentin, type I collagen, and BMP receptors, heterodimeric structures expressed on mesenchymal lineage cells. In addition, BMPCs stain with anti-CD105 (endoglin), a putative marker for bone-marrow mesenchymal stem cells (MSCs).
authors.affiliation: Department of Medicine, University of California, San Diego, CA 92093-0664, USA. nzfaifler@ucsd.edu
authors.forename: N J
authors.initials: NJ
authors.lastname: Zvaifler
authors.forename: L
authors.initials: L
authors.lastname: Marinova-Mutafchieva
authors.forename: G
authors.initials: G
authors.lastname: Adams
authors.forename: C J
authors.initials: CJ
authors.lastname: Edwards
authors.forename: J
authors.initials: J
authors.lastname: Moss
authors.forename: J A
authors.initials: JA
authors.lastname: Burger
authors.forename: R N
authors.initials: RN
authors.lastname: Maini
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood; D018952:Antigens, CD34 / Q000032:analysis; D001773:Blood Cells / Q000166:cytology*; D001854:Bone Marrow Cells / Q000166:cytology; D002452:Cell Count; D002469:Cell Separation; D054377:Chemokine CXCL12; D019743:Chemokines, CXC / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D008648:Mesoderm / Q000166:cytology*; D018715:Microscopy, Video; D012016:Reference Values; D013234:Stem Cells / Q000166:cytology*
chemical_list: D018952:Antigens, CD34; C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D019743:Chemokines, CXC; D000469:Alkaline Phosphatase