title: Hyperabsorption and retention of campestanol in a sitosterolemic homozygote: comparison with her mother and three control subjects.
abstract: We measured the percent absorption, turnover, and distribution of campestanol (24-methyl-5alpha-cholestan-3beta-ol) in a sitosterolemic homozygote, her obligate heterozygous mother, and three healthy human control subjects. For reasons relating to sterol hyperabsorption, the homozygote consumed a diet low in plant sterols that contained campestanol at about 2 mg/day. The heterozygote and three control subjects were fed a diet supplemented with a spread that contained campestanol at 540 mg/day and sitostanol (24-ethyl-5alpha-cholestan-3beta-ol) at 1.9 g/day as fatty acid esters. Plasma campestanol concentrations determined by capillary gas-liquid chromatography were 0.72 +/- 0.03 mg/dl in the homozygote, 0.09 +/- 0.04 mg/dl in the heterozygote, and 0.05 +/- 0.03 mg/dl for the control mean. After simultaneous pulse labeling with [3alpha-(3)H]campestanol intravenously and [23-(14)C]campestanol orally, the maximum percent absorption measured by the plasma dual-isotope ratio method as a single time point was 80% in the homozygote, 14.3% in the heterozygote, and 5.5 +/- 4.3% as the mean for three control subjects. Turnover (pool size) values estimated by mathematical analysis of the specific activity versus time [3alpha-(3)H]campestanol decay curves were as follows: 261 mg in the homozygote, 27.3 mg in the heterozygote, and 12.8 +/- 7.6 mg in the three control subjects (homogygote vs. controls, P < 0.001). The calculated production rate (mg/24 h) equivalent to actual absorption in the presence of dietary sterols and stanols was 0.67 mg/day or 31% of intake in the homozygote, 2.1 mg/day or 0.3% of intake in the heterozygote, and 0.7 +/- 0.3 mg/day or 0.1% of intake in the three control subjects. However, the excretion constant from pool A (K(A)) was prolonged markedly in the homozygote, but was 100 times more rapid in the heterozygote and three control subjects.Thus, campestanol, like other noncholesterol sterols, is hyperabsorbed and retained in sitosterolemic homozygotes. However, campestanol absorption was only slightly increased in the sitosterolemic heterozygote and removal was as rapid as in control subjects.
authors.affiliation: Gastroenterology Research Laboratory, Department of Veterans Affairs, New Jersey Heath Care System, East Orange, NJ 07018, USA.
authors.forename: G
authors.initials: G
authors.lastname: Salen
authors.forename: G
authors.initials: G
authors.lastname: Xu
authors.forename: G S
authors.initials: GS
authors.lastname: Tint
authors.forename: A K
authors.initials: AK
authors.lastname: Batta
authors.forename: S
authors.initials: S
authors.lastname: Shefer
mesh_terms: D000293:Adolescent; D000328:Adult; D002250:Carbon Radioisotopes; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000097:blood; D004032:Diet; D005260:Female; D006207:Half-Life; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D007408:Intestinal Absorption; D007700:Kinetics; D008052:Lipid Metabolism, Inborn Errors / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D010840:Phytosterols / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism*; D012855:Sitosterols / Q000008:administration & dosage / Q000097:blood*; D014316:Tritium
chemical_list: D002250:Carbon Radioisotopes; D010840:Phytosterols; D012855:Sitosterols; D014316:Tritium; C003535:campestanol; C021273:campesterol; C025473:gamma-sitosterol; D002784:Cholesterol; C021255:stigmastanol