Ascorbic acid stimulation of production of a highly branched ,beta-1,3-glucan by Aureobasidium pullulans K-1--oxalic acid, a metabolite of ascorbic acid as the stimulating substance.
The production of a highly branched beta-1,3-glucan by Aureobasidium pullulans K-1 in Czapek's medium has been found to be stimulated by ascorbic acid. When the culture supernatant, after removal of polysaccharide from the culture filtrate by ethanol precipitation, was concentrated, then added to a new medium and this strain was cultured in the medium, the polysaccharide production was stimulated the same as when L-ascorbic acid was added to the medium. The stimulating substance was partially purified from the supernatant, and was found to be oxalic acid; 0.03% oxalic acid was the most effective concentration for the stimulation of polysaccharide production. The stimulating substance, oxalic acid, was proved to be derived from ascorbic acid added to a medium in an experiment using L-[1-14C]ascorbic acid. We suggest that oxalic acid generated from the metabolism of ascorbic acid in cells of Aureobasidium pullulans K-1 participated in the stimulation of the polysaccharide production by ascorbic acid.
Hamada
N
N
Osaka Municipal Technical Research Institute, Japan.
Deguchi
K
K
Ohmoto
T
T
Sakai
K
K
Ohe
T
T
Yoshizumi
H
H
D001205:Ascorbic Acid / Q000378:metabolism / Q000494:pharmacology*; D005936:Glucans / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D003904:Mitosporic Fungi / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D019815:Oxalic Acid / Q000494:pharmacology*; D047071:beta-Glucans
D005936:Glucans; D047071:beta-Glucans; C033363:beta-1,3-glucan; D019815:Oxalic Acid; D001205:Ascorbic Acid