[Growth and metabolism of hybridoma cells cultured in flask and spinner bottles].
The growth and metabolism of WuT3 hybridoma cells were very different when cells were cultured in flask and spinner bottles. In the flask bottles, a longer culture duration and the higher cell density were met, whereas in the spinner bottles the more vigorous cell metabolism took place, resulting in that either the specific consumption rates of glucose and amino acids or the specific production rates of lactate, ammonia and alanine were higher.
Zhang
L
L
State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai.
Shen
H
H
Zhang
Y X
YX
D000596:Amino Acids / Q000378:metabolism; D000641:Ammonia / Q000378:metabolism; D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D005947:Glucose / Q000378:metabolism; D006825:Hybridomas / Q000166:cytology* / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C
D000596:Amino Acids; D019344:Lactic Acid; D000641:Ammonia; D005947:Glucose