title: Rotavirus infection induces cytoskeleton disorganization in human intestinal epithelial cells: implication of an increase in intracellular calcium concentration.
abstract: Rotavirus infection is the most common cause of severe infantile gastroenteritis worldwide. In vivo, rotavirus exhibits a marked tropism for the differentiated enterocytes of the intestinal epithelium. In vitro, differentiated and undifferentiated intestinal cells can be infected. We observed that rotavirus infection of the human intestinal epithelial Caco-2 cells induces cytoskeleton alterations as a function of cell differentiation. The vimentin network disorganization detected in undifferentiated Caco-2 cells was not found in fully differentiated cells. In contrast, differentiated Caco-2 cells presented Ca(2+)-dependent microtubule disassembly and Ca(2+)-independent cytokeratin 18 rearrangement, which both require viral replication. We propose that these structural alterations could represent the first manifestations of rotavirus-infected enterocyte injury leading to functional perturbations and then to diarrhea.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, Unité 510, Faculté de Pharmacie, Université Paris XI, 92296 Ch atenay-Malabry cedex, France. jean-philippe.brunet@cep.u-psud.fr
authors.forename: J P
authors.initials: JP
authors.lastname: Brunet
authors.forename: N
authors.initials: N
authors.lastname: Jourdan
authors.forename: J
authors.initials: J
authors.lastname: Cotte-Laffitte
authors.forename: C
authors.initials: C
authors.lastname: Linxe
authors.forename: M
authors.initials: M
authors.lastname: Géniteau-Legendre
authors.forename: A
authors.initials: A
authors.lastname: Servin
authors.forename: A M
authors.initials: AM
authors.lastname: Quéro
mesh_terms: D018938:Caco-2 Cells; D002118:Calcium / Q000378:metabolism*; D002454:Cell Differentiation; D003599:Cytoskeleton / Q000648:ultrastructure*; D020895:Enterocytes / Q000166:cytology / Q000648:ultrastructure / Q000821:virology*; D006801:Humans; D012401:Rotavirus / Q000472:pathogenicity* / Q000502:physiology; D014779:Virus Replication
chemical_list: D002118:Calcium