title: Molecular confirmation of human immunodeficiency virus (HIV) type 2 in HIV-seropositive subjects in south India.
abstract: Nested PCRs for human immunodeficiency virus type 1 (HIV-1) and HIV-2 were compared with immunoblot test results. Twelve of 13 immunoblot-positive HIV-2 samples were positive by PCR. There were five INNO-LIA (Innogenetics, Zwijnaarde, Belgium) and/or HIVBLOT 2.2 (Genelabs, Singapore) samples that tested positive for dual infection. HIV-1 PCR was positive in all samples, while HIV-2 PCR was positive in two and RIBA (Chiron Corporation, San Diego, Calif.) was positive for HIV-2 in three samples. Thus the prevalence of HIV-2 is accurately estimated by the use of immunoblotting, but that of HIV-1 and -2 dual infection may be overestimated.
authors.affiliation: Departments of Clinical Virology, Christian Medical College Hospital, Vellore, India.
authors.forename: R
authors.initials: R
authors.lastname: Kannangai
authors.forename: S
authors.initials: S
authors.lastname: Ramalingam
authors.forename: K J
authors.initials: KJ
authors.lastname: Prakash
authors.forename: O C
authors.initials: OC
authors.lastname: Abraham
authors.forename: R
authors.initials: R
authors.lastname: George
authors.forename: R C
authors.initials: RC
authors.lastname: Castillo
authors.forename: D H
authors.initials: DH
authors.lastname: Schwartz
authors.forename: M V
authors.initials: MV
authors.lastname: Jesudason
authors.forename: G
authors.initials: G
authors.lastname: Sridharan
mesh_terms: D005260:Female; D006679:HIV Seropositivity / Q000453:epidemiology / Q000821:virology*; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification; D015498:HIV-2 / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D015151:Immunoblotting; D007194:India / Q000453:epidemiology; D008297:Male; D017720:Molecular Epidemiology; D016133:Polymerase Chain Reaction