Quantitation of aliphatic suberin in Quercus suber L. cork by FTIR spectroscopy and solid-state (13)C-NMR spectroscopy.
This work determined that the percentage of suberin in cork may be found by solid-state (13)C cross polarization/magic angle spinning (CP/MAS) NMR spectroscopy and by FTIR with photoacoustic detection (FTIR-PAS) spectroscopy. A linear relationship is found between the suberin content measured through CP/MAS spectral areas and that measured gravimetrically. Furthermore, application of a partial least squares (PLS1) regression model to the NMR and gravimetric data sets clearly correlates the two sets, enabling suberin quantification with 90% precision. Suberin quantitation by FTIR-PAS spectroscopy is also achieved by a PLS1 regression model, giving 90% accurate estimates of the percentage of suberin in cork. Therefore, (13)C-CP/MAS NMR and FTIR-PAS proved to be useful and accurate noninvasive techniques to quantify suberin in cork, thus avoiding the traditional time consuming and destructive chemical methods.
Lopes
M H
MH
Departamento de Química, Universidade de Aveiro, 3810 Aveiro, Portugal.
Neto
C P
CP
Barros
A S
AS
Rutledge
D
D
Delgadillo
I
I
Gil
A M
AM
D002247:Carbon Isotopes; D008055:Lipids; D009682:Magnetic Resonance Spectroscopy; D008563:Membrane Lipids / Q000032:analysis* / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D014197:Trees / Q000737:chemistry*
D002247:Carbon Isotopes; D008055:Lipids; D008563:Membrane Lipids; C065875:suberin