Expression of brain/kidney protein in Müller cells of rat retina following transient ischemia.
We have investigated the expression and cellular localization of brain/kidney (B/K) protein in the rat retina following transient ischemia. In the normal retina, strong B/K immunoreactivity was localized to some ganglion cells. In addition, a few radial Muller cell processes showed B/K immunoreactivity. Following ischemia and reperfusion, most B/K-labeled ganglion cells were lost, whereas between 1 day and 2 weeks post-lesion B/K immunoreactivity appeared in many more Muller cell processes with increasing intensity. Quantitative evaluation by immunoblotting confirmed that B/K expression then decreased progressively, to 35% of control values at four weeks post-lesion. Our findings suggest that Muller cells are involved in the pathophysiology of retinal ischemia through the expression of B/K following transient ischemia.
Ju
W K
WK
Department of Anatomy, College of Medicine, The Catholic University of Korea, Socho-gu, Seoul.
Choi
S H
SH
Kwon
J S
JS
Kwon
O J
OJ
Lee
M Y
MY
Oh
S J
SJ
Moon
J L
JL
Chun
M H
MH
D000818:Animals; D015151:Immunoblotting; D007511:Ischemia / Q000378:metabolism* / Q000473:pathology; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology; D012160:Retina / Q000378:metabolism* / Q000473:pathology; D012165:Retinal Ganglion Cells / Q000378:metabolism*; D012171:Retinal Vessels; D050857:Synaptotagmins; D013997:Time Factors; D014018:Tissue Distribution
D009419:Nerve Tissue Proteins; C409229:Syt17 protein, rat; D050857:Synaptotagmins