title: Equine infectious anaemia virus proteins with epitopes most frequently recognized by cytotoxic T lymphocytes from infected horses.
abstract: Efficacious lentiviral vaccines designed to induce cytotoxic T lymphocytes (CTL) in outbred populations with a diverse repertoire of MHC class I molecules should contain or express multiple viral proteins. To determine the equine infectious anaemia virus (EIAV) proteins with epitopes most frequently recognized by CTL from seven horses infected for 0.5 to 7 years, retroviral vector-transduced target cells expressing viral proteins were used in CTL assays. Gag p15 was recognized by CTL from 100% of these infected horses. p26 was recognized by CTL from 86%, SU and the middle third of Pol protein were each recognized by 43%, TM by 29%, and S2 by 14%. Based on these results, it is likely that a construct expressing the 359 amino acids constituting p15 and p26 would contain epitopes capable of stimulating CTL in most horses.
authors.affiliation: Department of Veterinary Microbiology and Pathology1 and Department of Animal Sciences2, Washington State University, Pullman, WA 99164-7040, USA.
authors.forename: Travis C
authors.initials: TC
authors.lastname: McGuire
authors.affiliation: Department of Veterinary Microbiology and Pathology1 and Department of Animal Sciences2, Washington State University, Pullman, WA 99164-7040, USA.
authors.forename: Steven R
authors.initials: SR
authors.lastname: Leib
authors.affiliation: Department of Veterinary Microbiology and Pathology1 and Department of Animal Sciences2, Washington State University, Pullman, WA 99164-7040, USA.
authors.forename: Scott M
authors.initials: SM
authors.lastname: Lonning
authors.affiliation: Department of Veterinary Microbiology and Pathology1 and Department of Animal Sciences2, Washington State University, Pullman, WA 99164-7040, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Veterinary Microbiology and Pathology1 and Department of Animal Sciences2, Washington State University, Pullman, WA 99164-7040, USA.
authors.forename: Katherine M
authors.initials: KM
authors.lastname: Byrne
authors.affiliation: Department of Veterinary Microbiology and Pathology1 and Department of Animal Sciences2, Washington State University, Pullman, WA 99164-7040, USA.
authors.forename: Robert H
authors.initials: RH
authors.lastname: Mealey
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D000939:Epitopes / Q000276:immunology*; D004859:Equine Infectious Anemia / Q000276:immunology*; D006736:Horses / Q000276:immunology / Q000821:virology; D004860:Infectious Anemia Virus, Equine / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D000956:Antigens, Viral; D000939:Epitopes