title: [Role of protein kinase A and calcium ions in regulating ovulation of oocytes by gonadotropins in the grass frog (Rana temporaria) in vitro].
abstract: The inhibitor of cAMP-dependent protein kinase N-[2-(methylamino)ethyl]-5-isoquinolinesulfonamide (H-8) (12.5-50 microM) decreased the rate of ovulation of the follicle-enclosed Rana temporaria oocytes induced by the homologous pituitary extract in amphibian Ringer solution and in a chloride-free medium. The inhibitor of voltage-dependent calcium channels diltiazem (10 and 100 microM) decreased the rate of ovulation in Ringer solution but did not affect it in a chloride-free medium or decreased the ovulation inhibitory effect of this medium. It was concluded that cAMP-dependent protein kinase and intracellular free calcium ions were involved as second messengers in the gonadotropin regulation not only in maturation of amphibian oocytes but also in ovulation.
authors.affiliation: Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: M N
authors.initials: MN
authors.lastname: Skoblina
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D004110:Diltiazem / Q000494:pharmacology; D005260:Female; D006062:Gonadotropins / Q000502:physiology*; D066298:In Vitro Techniques; D007477:Ions; D010060:Ovulation / Q000502:physiology*; D010902:Pituitary Gland / Q000502:physiology; D011896:Rana temporaria / Q000502:physiology*
chemical_list: D002121:Calcium Channel Blockers; D006062:Gonadotropins; D007477:Ions; D017868:Cyclic AMP-Dependent Protein Kinases; D004110:Diltiazem; D002118:Calcium