Optimization of conditions for safflower cell culture and accumulation of cellicolous product tocopherols.
The culture of calli, which were induced from cotyledon of Carthamus tinctorius L, was able to synthesize tocopherols. Results determined by NP-HPLC showed that the main component was alpha-tocopherol. When 0.1% casein was added into MS medium, and 2,4-D concentration was adjucted to 0.30 mg/L and 6-BA to 1.80 mg/L, alpha-, beta- and gamma-forms of tocopherol reached 4.1706, 0.3120 and 0.1650 microg/g fresh calli, respectively. The effective tocopherol content was 4.4091 microg/g fresh calli, and was 57 times higher than that in the control.
Wang
P
P
Bio-environment Engineering Institute of Zhejiang University, Huajiachi, Hangzhou, China.
Ou
S
S
Peilin
C
C
D019659:Asteraceae / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods*; D014810:Vitamin E / Q000096:biosynthesis*
D014810:Vitamin E