[cDNA cloning, sequence analysis of trehalose-6-phosphate synthase gene from Saccharomyces cerevisiae AS2.1416].
The trehalose-6-phosphate synthase gene tps1 was amplified from yeast S. cerevisiae AS2.1416 cDNA library by polymerase chain reaction. This 1.5 kb fragment was cloned into PstI and BamHI sites of pGEM-T easy vector and the sequence of the gene indicated the cloned tps1 gene contained 1485 nucleotides encoding for 495 amino acid and shared a sequence homology of 99.6% with that from S. cerevisiae S288C.
Dong
Z Y
ZY
Enzymology Department, Chinese Academy of Sciences, Beijing.
Duan
Y K
YK
Chen
H M
HM
Jin
C
C
Zhang
S Z
SZ
D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D015723:Gene Library; D005964:Glucosyltransferases / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D018076:DNA, Complementary; D005964:Glucosyltransferases; C023180:trehalose-6-phosphate synthase