Evaluation of a commercial DNA enzyme immunoassay for detection of enterovirus reverse transcription-PCR products amplified from cerebrospinal fluid specimens.
We evaluated the DiaSorin DNA enzyme immunoassay (DEIA) kit for detection of enteroviral reverse transcription-PCR (RT-PCR) products amplified from cerebrospinal fluid. By use of an optical density of 0.05 as the absorbance cutoff, 35% of 198 specimens were PCR positive, whereas 16% were culture positive. DEIA was rapid and sensitive and can help implement enterovirus RT-PCR in clinical laboratories.
Young
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PP
The Departments of Pathology, Washington University School of Medicine at St. Louis Children's Hospital, St. Louis, Missouri 63110, USA.
Buller
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RS
Storch
G A
GA
D000293:Adolescent; D000328:Adult; D002555:Cerebrospinal Fluid / Q000821:virology*; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis*; D004770:Enterovirus / Q000235:genetics / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000821:virology*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D007223:Infant; D008587:Meningitis, Viral / Q000821:virology*; D011933:Reagent Kits, Diagnostic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014776:Virus Cultivation / Q000379:methods
D004279:DNA, Viral; D011933:Reagent Kits, Diagnostic