Extracellular matrix protein gene expression of bovine chondrocytes cultured on resorbable scaffolds.
It has been demonstrated that using cultured chondrocytes that have been seeded onto various biomatrices can enhance the quality of the articular cartilage repair tissue. As tissue-engineering becomes increasingly more complex there is a need to understand how a specific biomaterial may influence gene expression. In this study several commonly used scaffold materials for cartilage tissue engineering were evaluated with respect to their influence on matrix gene expression. Primary cultures of bovine chondrocytes were established in monolayer then seeded onto polylactic acid (PLLA), polyglycolic acid (PGA), collagen matrices. The induction of collagen type I, collagen type II, and aggrecan was observed at various time points on these biomaterials using RT-PCR. The collagen type I gene was upregulated on collagen scaffolds throughout the culture period. PLLA and PGA showed initial induction followed by downregulation. Monolayer culture did not induce collagen I message. Collagen II genes were selectively upregulated after 72 and 96 h post seeding depending the scaffold material. Monolayer culture had strong induction of collagen II. The aggrecan protein was consistently expressed in all scaffold materials cultures and monolayer.
Saldanha
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Department of Surgeon, Division of Orthopaedics, North Shore University Hospital, Manhasset, NY 11030, USA.
Grande
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DA
D000818:Animals; D001483:Base Sequence; D001672:Biocompatible Materials; D002417:Cattle; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism*; D003094:Collagen; D017931:DNA Primers; D016326:Extracellular Matrix Proteins / Q000235:genetics*; D015870:Gene Expression; D019344:Lactic Acid; D011091:Polyesters; D011100:Polyglycolic Acid; D011108:Polymers; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction
D001672:Biocompatible Materials; D017931:DNA Primers; D016326:Extracellular Matrix Proteins; D011091:Polyesters; D011108:Polymers; D011100:Polyglycolic Acid; D019344:Lactic Acid; C033616:poly(lactide); D003094:Collagen