Misoprostol induces relaxation of human corpus cavernosum smooth muscle: comparison to prostaglandin E1.
Prostaglandin E1 (PGE1) relaxes trabecular smooth muscle by interacting with specific G-protein coupled receptors on human corpus cavernosum smooth muscle and increasing intracellular synthesis of cAMP. Misoprostol (Cytotec), is an oral prostaglandin E analogue. The purpose of this study was to compare the functional activity of misoprostol with PGE1 in human corpus cavernosum and cultured human corpus cavernosum smooth muscle cells. Misoprostol, misoprostol free acid or PGE1 induced dose-dependent relaxations in strips of human corpus cavernosum. At concentrations greater than 10(-6) M, tissue recontraction was observed with all three agents. This was abrogated by pretreatment with the thromboxane A2 receptor antagonist SQ29,548. From these observations, we conclude that misoprostol is activated by human corpus cavernosum in situ and relaxes phenylephrine-precontrated tissue strips in vitro. This relaxation response is mediated by the increased cAMP synthesis by these agents.
Moreland
R B
RB
Department of Urology, Boston University School of Medicine, MA 02118, USA.
Kim
N N
NN
Nehra
A
A
Parulkar
B G
BG
Traish
A
A
D000527:Alprostadil / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology*; D019086:Bridged Bicyclo Compounds, Heterocyclic; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D005231:Fatty Acids, Unsaturated; D006801:Humans; D006834:Hydrazines / Q000494:pharmacology; D008297:Male; D016595:Misoprostol / Q000008:administration & dosage / Q000494:pharmacology*; D009126:Muscle Relaxation / Q000187:drug effects*; D009132:Muscles / Q000502:physiology*; D010413:Penis / Q000502:physiology*; D017482:Receptors, Thromboxane / Q000037:antagonists & inhibitors
D019086:Bridged Bicyclo Compounds, Heterocyclic; D005231:Fatty Acids, Unsaturated; D006834:Hydrazines; D017482:Receptors, Thromboxane; D016595:Misoprostol; C045749:SQ 29548; D000242:Cyclic AMP; D000527:Alprostadil