title: Induction of porcine cytokine mRNA expression after DNA immunization and pseudorabies virus infection.
abstract: Injection of plasmid DNA encoding pseudorabies virus (PRV) glycoprotein into pig muscle has been shown to result in protective immunity against lethal infection. Here, pigs were vaccinated by a single coinjection of three plasmids encoding PRV glycoproteins gB, gC, and gD, with plasmid expressing porcine granulocytemacrophage colony-stimulating factor (GM-CSF) or porcine interferon-alpha (IFN-alpha). DNA immunization induced a primary T cell-mediated response characterized by low rates of IFN-gamma, interleukin-2 (IL-2), and IL4 mRNA in peripheral blood mononuclear cells (PBMC). Very low rates of PRV-specific IgG1 and the absence of IgG2 were obtained. Codelivery of plasmid expressing GM-CSF or IFN-alpha had no effect on cytokine mRNA expression or on B cell response. After a high virulent challenge, high levels of cytokine mRNA, mainly IFN-gamma, and high secondary antibody (Ab) response were induced in all DNA-vaccinated pigs. Codelivery of GMCSF gene significantly increased both Th immune response (i.e., IFN-gamma and IL-4 mRNA expression) and clinical protection but had no effect on secondary B immune response. Codelivery of IFN-alpha gene had no beneficial effect on secondary T and B cell immune responses.
authors.affiliation: Service de Biologie Moléculaire, AFSSA, Ploufragan, France. v.dufour@plofragan.afssa.fr
authors.forename: V
authors.initials: V
authors.lastname: Dufour
authors.forename: S
authors.initials: S
authors.lastname: Chevallier
authors.forename: R
authors.initials: R
authors.lastname: Cariolet
authors.forename: S
authors.initials: S
authors.lastname: Somasundaram
authors.forename: F
authors.initials: F
authors.lastname: Lefevre
authors.forename: A
authors.initials: A
authors.lastname: Jestin
authors.forename: E
authors.initials: E
authors.lastname: Albina
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D011558:Herpesvirus 1, Suid / Q000235:genetics / Q000276:immunology*; D011557:Pseudorabies / Q000235:genetics / Q000276:immunology* / Q000517:prevention & control; D022421:Pseudorabies Vaccines / Q000235:genetics / Q000276:immunology*; D012333:RNA, Messenger / Q000096:biosynthesis*; D013552:Swine; D013553:Swine Diseases / Q000235:genetics / Q000276:immunology / Q000517:prevention & control; D019444:Vaccines, DNA / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D016207:Cytokines; D022421:Pseudorabies Vaccines; D012333:RNA, Messenger; D019444:Vaccines, DNA