title: The Bacillus subtilis 168 csn gene encodes a chitosanase with similar properties to a streptomyces enzyme.
abstract: The Bacillus subtilis 168 csn gene encodes a chitosanase. It was found that transcription of the csn gene was temporally regulated and was not subject to metabolic repression. Chitosanase synthesis was abolished in a csn mutant strain. Csn was overproduced in B. subtilis, partially purified and characterized. The deduced amino acid sequence, K(m), and optimal pH and temperature of the B. subtilis enzyme were closer to those of a chitosanase from Streptomyces sp. N174 than to those of chitosanases from other Bacillus strains.
authors.affiliation: Centro Nacional de Biotecnologı́a (CSIC), Campus de la Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain1.
authors.forename: Luis A
authors.initials: LA
authors.lastname: Rivas
authors.affiliation: Centro Nacional de Biotecnologı́a (CSIC), Campus de la Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain1.
authors.forename: Vı Ctor
authors.initials: VC
authors.lastname: Parro
authors.affiliation: Centro Nacional de Biotecnologı́a (CSIC), Campus de la Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain1.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Moreno-Paz
authors.affiliation: Centro Nacional de Biotecnologı́a (CSIC), Campus de la Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain1.
authors.forename: Rafael P
authors.initials: RP
authors.lastname: Mellado
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D005798:Genes, Bacterial; D006026:Glycoside Hydrolases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D014176:Protein Biosynthesis; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; D012329:RNA, Bacterial; D006026:Glycoside Hydrolases; C019868:chitosanase