[On-line measurement of oxygen uptake rate in the cultivation of Vero cells using the dynamic method].
The oxygen uptake rate(OUR) during the cultivation of Vero cells in 1.5 L CelliGen bioreactor was on-line determined using the dynamic method. The results showed that the cell growth and metabolic state during the exponential growth phase was lineally related to the OUR. This implies that the on-line measurement of OUR can be used to promptly monitor the physiological state of cultured cells and to efficiently avoid contamination because of frequent sampling in the large-scale cultivation of mammalian cells.
Zhou
Y J
YJ
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai.
Tan
W S
WS
Zhao
J
J
Hua
P
P
Sun
X M
XM
Yu
J T
JT
D000818:Animals; D002452:Cell Count; D002522:Chlorocebus aethiops; D005947:Glucose / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D010101:Oxygen Consumption; D014709:Vero Cells
D019344:Lactic Acid; D005947:Glucose