[Sensitized photomodification of DNA with binary sysytems of oligonucleotide conjugates. VI. Effect of substituents in the anthracene residue of the sensitizer].
Photomodification of ssDNA by binary systems of oligonucleotide conjugates complementary to the adjacent sequences of the target DNA was studied. One of the conjugates comprised a substituted anthracene as a sensitizer; the other, p-azidotetrafluorobenzaldehyde 3-aminopropionylhydrazone as a photoreagent. The sensitized photomodification is initiated by the 365-580-nm light through an efficient energy transfer from the photoexcitated sensitizer onto the photoreagent in a complementary complex of the binary system with the DNA target where the sensitizer and the photoreagent are sterically converged. Influence of substituents in the anthracene residue on the efficiency of the DNA sensitized photomodification was considered. The oligonucleotide conjugate of anthracene-9-al 3-aminopropionylhydrazone allows highly specific initiation of the sensitized photomodification upon irradiation with visible light at > 460 nm in conditions generating no photoreaction in the sensitizer's absence.
Dobrikov
M I
MI
Institue of Bioorganic Chemistry, Siberian Division, Russian Academy of Science, Novosibirsk, Russia.
Gaĭnutdinov
T U
TU
Ivanova
T M
TM
Vlasov
V V
VV
D000873:Anthracenes / Q000737:chemistry*; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D010777:Photochemistry
D000873:Anthracenes; D009841:Oligonucleotides; D004247:DNA