DNA triplex structures are stabilized by the incorporation of 3'-endo blocked pyrimidine nucleosides in the Hoogsteen strand.
A short route to pyrimidine locked nucleosides has been developed for their incorporation in triplex forming oligonucleotides (TFO). Compared to oligonucleotides built with standard nucleosides, the modified TFOs containing 3'-endo blocked residues formed, with their corresponding DNA duplexes, more stable triple helix systems, an effect which might be ascribed to the 3'-endo pucker of the modified nucleoside residues.
Savy
P
P
Institut de Chimie des Substances Naturelles, CNRS, Gif-sur-Yvette, France.
Benhida
R
R
Fourrey
J L
JL
Maurisse
R
R
Sun
J S
JS
D001483:Base Sequence; D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry*; D004355:Drug Stability; D008968:Molecular Conformation; D009690:Nucleic Acid Conformation; D009711:Nucleotides / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D011741:Pyrimidine Nucleosides / Q000737:chemistry*; D013329:Structure-Activity Relationship
C419822:ADP-S-HBES-S-dGTP; D009711:Nucleotides; D009838:Oligodeoxyribonucleotides; D011741:Pyrimidine Nucleosides; D004247:DNA