Screening of several H-2 congenic mouse strains identified H-2(q) mice as highly susceptible to MOG-induced EAE with minimal adjuvant requirement.
We identified H-2(q) as a susceptible genotype for MOG-induced EAE by systematic screening of a series of H-2 congenic B10 mouse strains. A series of H-2(q)-bearing strains with divergent gene backgrounds were subsequently investigated. DBA/1 mice were highly susceptible to MOG(1-125)- and MOG(79-96)-induced EAE in the absence of pertussis toxin. Immunisation with MOG(1-125) and MOG(79-96) induced an autoreactive T-cell response in DBA/1 mice. Brain histopathology revealed T-cell and macrophage-infiltrated lesions with associated demyelination. The important features which make this an appropriate model of human disease are high sensitivity to MOG and dependence of an immunodominant peptide region homologous to that implicated in multiple sclerosis.
Abdul-Majid
K B
KB
Neuroimmunology Unit, L8:04 Centre for Molecular Medicine, Karolinska Hospital, SE-17176, Stockholm, Sweden. khairul.majid@cmm.ki.se
Jirholt
J
J
Stadelmann
C
C
Stefferl
A
A
Kjellén
P
P
Wallström
E
E
Holmdahl
R
R
Lassmann
H
H
Olsson
T
T
Harris
R A
RA
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D001323:Autoantibodies / Q000276:immunology; D002455:Cell Division / Q000276:immunology; D004195:Disease Models, Animal; D004198:Disease Susceptibility / Q000276:immunology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000139:chemically induced* / Q000276:immunology* / Q000473:pathology; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D007114:Immunization / Q000379:methods; D016056:Immunodominant Epitopes / Q000276:immunology; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D051379:Mice; D020297:Mice, Congenic / Q000276:immunology*; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein / Q000276:immunology* / Q000494:pharmacology; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments / Q000276:immunology / Q000494:pharmacology; D037342:Pertussis Toxin; D013116:Spinal Cord / Q000276:immunology / Q000473:pathology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D000276:Adjuvants, Immunologic; D001323:Autoantibodies; D018984:Epitopes, T-Lymphocyte; D016056:Immunodominant Epitopes; C571326:MOG protein, human; C571327:Mog protein, mouse; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments; D010566:Virulence Factors, Bordetella; D037342:Pertussis Toxin