cDNA cloning, chromosomal mapping, and expression analysis of human VE-Cadherin-2.
Murine vascular endothelial cadherin-2 (VE-cad-2) is a cellular adhesion molecule that is distinct from vascular endothelial cadherin 1 (VE-cad-1) in that it does not interact with catenins and does not appear to affect cell migration or growth. In this study, we have cloned a full-length cDNA of the human homolog of VE-cad-2 and used it to map the chromosomal locus of the VE-cad-2 gene. Human VE-cad-2 maps to Chromosome (Chr) 5q31. The cDNA of human VE-cad-2 is highly homologous to mouse VE-cad-2, except for a C-terminal tail. The genomic structure of VE-cad-2 is strikingly similar to that reported for a large family of neuronal protocadherin genes mapped to Chr 5q, yet the amino acid sequences between VE-cad-2 and the protocadherins are substantially divergent. The promoter of human VE-cad-2 contains two TATA boxes and transcription initiates from a single site 3' to these elements. Similar to mouse VE-cad-2, the human gene is expressed primarily in highly vascularized tissues.
Ludwig
D
D
ImClone Systems Incorporated, New York, New York 10014, USA.
Lorenz
J
J
Dejana
E
E
Bohlen
P
P
Hicklin
D J
DJ
Witte
L
L
Pytowski
B
B
D020121:5' Untranslated Regions; D000595:Amino Acid Sequence; D001483:Base Sequence; D015820:Cadherins / Q000235:genetics* / Q000378:metabolism*; D002874:Chromosome Mapping; D002895:Chromosomes, Human, Pair 5; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004730:Endothelium, Vascular / Q000502:physiology*; D005091:Exons; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D000091363:Protocadherins; D016385:TATA Box
D020121:5' Untranslated Regions; D015820:Cadherins; D018076:DNA, Complementary; C113432:PCDH12 protein, human; D000091363:Protocadherins