title: Detection and isolation of coronavirus from feces of three herds of feedlot cattle during outbreaks of winter dysentery-like disease.
abstract: Clinical signs of a winter dysentery-like syndrome in 6- to 9-month-old cattle in 3 feedlots included acute onset of diarrhea with high morbidity and low mortality, respiratory tract problems that included dyspnea, coughing, and nasal discharge, and high rectal temperatures. Bovine coronavirus was detected by use of an ELISA and immune electron microscopy in fecal and nasal swab samples and by immunohistochemical analysis of intestinal sections collected from calves during necropsy. Bovine coronavirus should be considered in the differential diagnoses for diseases that cause acute onset of bloody diarrhea in feedlot cattle.
authors.affiliation: Department of Veterinary Preventive Medicine, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster 44691-4096, USA.
authors.forename: K O
authors.initials: KO
authors.lastname: Cho
authors.forename: P G
authors.initials: PG
authors.lastname: Halbur
authors.forename: J D
authors.initials: JD
authors.lastname: Bruna
authors.forename: S D
authors.initials: SD
authors.lastname: Sorden
authors.forename: K J
authors.initials: KJ
authors.lastname: Yoon
authors.forename: B H
authors.initials: BH
authors.lastname: Janke
authors.forename: K O
authors.initials: KO
authors.lastname: Chang
authors.forename: L J
authors.initials: LJ
authors.lastname: Saif
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D002478:Cells, Cultured; D018352:Coronavirus Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D017938:Coronavirus, Bovine / Q000276:immunology / Q000302:isolation & purification* / Q000648:ultrastructure; D003937:Diagnosis, Differential; D004196:Disease Outbreaks / Q000662:veterinary; D004403:Dysentery / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005243:Feces / Q000821:virology*; D005260:Female; D007420:Intestine, Large / Q000473:pathology / Q000821:virology; D008168:Lung / Q000821:virology; D008198:Lymph Nodes / Q000821:virology; D008297:Male; D016253:Microscopy, Immunoelectron / Q000662:veterinary; D012004:Rectal Neoplasms; D012141:Respiratory Tract Infections / Q000175:diagnosis / Q000662:veterinary / Q000821:virology; D014407:Tumor Cells, Cultured
chemical_list: D000956:Antigens, Viral