The inhibitory action of butyrate on lipopolysaccharide-induced nitric oxide production in RAW 264.7 murine macrophage cells.
The effect of butyrate, a natural bacterial product of colonic bacterial flora, on lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW 264.7 murine macrophage cells was studied. Butyrate significantly reduced NO production in LPS-stimulated RAW cells. The inhibition was abolished by the removal of butyrate. Butyrate also inhibited the expression of inducible type NO synthase (iNOS) in LPS-stimulated RAW cells. Furthermore, butyrate prevented the activation of nuclear factor (NF)-kappaB through the stabilization of IkappaB-alpha and IkappaB-beta. Butyrate did not affect the phosphorylation of mitogen-activated protein (MAP) kinases by LPS. It was, therefore, suggested that butyrate down-regulated LPS-induced NO production in RAW cells through preventing the expression of iNOS, and that it was due to the inhibitory action of butyrate on the activation of NF-kappaB.
Chakravortty
D
D
Department of Microbiology and Immunology, Research Center for Infectious Disease, Aichi Medical University, Nagakute, Japan.
Koide
N
N
Kato
Y
Y
Sugiyama
T
T
Mu
M M
MM
Yoshida
T
T
Yokochi
T
T
D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism; D002087:Butyrates / Q000494:pharmacology*; D002460:Cell Line; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism*; D010766:Phosphorylation
D001426:Bacterial Proteins; D002087:Butyrates; D008070:Lipopolysaccharides; C095564:Map protein, Staphylococcus aureus; D016328:NF-kappa B; D009569:Nitric Oxide