title: Fatty acid transport protein-1 mRNA expression in skeletal muscle and in adipose tissue in humans.
abstract: Fatty acid transporter protein (FATP)-1 mRNA expression was investigated in skeletal muscle and in subcutaneous abdominal adipose tissue of 17 healthy lean, 13 nondiabetic obese, and 16 obese type 2 diabetic subjects. In muscle, FATP-1 mRNA levels were higher in lean women than in lean men (2.2 +/- 0.1 vs. 0.6 +/- 0.2 amol/microg total RNA, P < 0.01). FATP-1 mRNA expression was decreased in skeletal muscle in obese women both in nondiabetic and in type 2 diabetic patients (P < 0.02 vs. lean women in both groups), and in all women there was a negative correlation with basal FATP-1 mRNA level and body mass index (r = -0.74, P < 0.02). In men, FATP-1 mRNA was expressed at similar levels in the three groups both in skeletal muscle (0.6 +/- 0.2, 0.6 +/- 0.2, and 0.8 +/- 0.2 amol/microg total RNA in lean, obese, and type 2 diabetic male subjects) and in adipose tissue (0.9 +/- 0.2 amol/microg total RNA in the 3 groups). Insulin infusion (3 h) reduced FATP-1 mRNA levels in muscle in lean women but not in lean men. Insulin did not affect FATP-1 mRNA expression in skeletal muscle in obese nondiabetic or in type 2 diabetic subjects nor in subcutaneous adipose tissue in any of the three groups. These data show a gender-related difference in the expression of the fatty acid transporter FATP-1 in skeletal muscle of lean individuals and suggest that changes in FATP-1 expression may not contribute to a large extent to the alterations in fatty acid uptake in obesity and/or type 2 diabetes.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale Unité-449, F-69372 Lyon, France.
authors.forename: C
authors.initials: C
authors.lastname: Binnert
authors.forename: H A
authors.initials: HA
authors.lastname: Koistinen
authors.forename: G
authors.initials: G
authors.lastname: Martin
authors.forename: F
authors.initials: F
authors.lastname: Andreelli
authors.forename: P
authors.initials: P
authors.lastname: Ebeling
authors.forename: V A
authors.initials: VA
authors.lastname: Koivisto
authors.forename: M
authors.initials: M
authors.lastname: Laville
authors.forename: J
authors.initials: J
authors.lastname: Auwerx
authors.forename: H
authors.initials: H
authors.lastname: Vidal
mesh_terms: D000005:Abdomen; D000273:Adipose Tissue / Q000737:chemistry*; D000328:Adult; D001786:Blood Glucose / Q000378:metabolism; D015992:Body Mass Index; D002352:Carrier Proteins / Q000235:genetics*; D003920:Diabetes Mellitus / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism; D050612:Fatty Acid Transport Proteins; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D015870:Gene Expression; D015309:Glucose Clamp Technique; D006801:Humans; D007328:Insulin / Q000097:blood; D008297:Male; D026901:Membrane Transport Proteins; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000737:chemistry*; D009765:Obesity / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012727:Sex Characteristics
chemical_list: D001786:Blood Glucose; D002352:Carrier Proteins; D050612:Fatty Acid Transport Proteins; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D026901:Membrane Transport Proteins; D012333:RNA, Messenger; C493568:SLC27A1 protein, human; C493573:SLC27A6 protein, human