Purification, crystallization and preliminary diffraction study of beta-galactosidase from Penicillium sp.
Crystals of an extracellular beta-galactosidase from Penicillium sp. (MW = 120 +/- 5 kDa) have been obtained from a sodium phosphate buffer using PEG as precipitant. The crystals belong to the tetragonal space group P4(1)or P4(3), with unit-cell parameters a = b = 110.82, c = 161.28 A, and diffract to 1.85 A resolution at a synchrotron source.
Neustroev
K N
KN
Petersburg Nuclear Physics Institute, Gatchina, St Petersburg, 188350, Russia.
de Sousa
E A
EA
Golubev
A M
AM
Brandão Neto
Eneyskaya
E V
EV
Kulminskaya
A A
AA
Polikarpov
I
I
D003460:Crystallization; D018360:Crystallography, X-Ray; D006031:Glycosylation; D010407:Penicillium / Q000201:enzymology*; D011487:Protein Conformation; D001616:beta-Galactosidase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism
D001616:beta-Galactosidase