[Comparative study on the chimeric ability of embryonic stem cell lines HDC and MESPU-13].
While producing transgenic mice by ES cell route, it is important to know whether ES cells used have a strong ability to produce chimeras or not. In this report, two methods were used for studying the chimeric ability of two ES cell lines (HDC and MESPU-13). One method was the blastocyst microinjection, that is, chimeras were produced by injecting 15 ES cells into the cavity of C57BL/6J blastocysts. Another was GPI electrophoresis for examinizing the chimerism of ES cells in internal tisssues and organs of chimeras. The results showed that the ability of producing chimeras of MESPU-13 cells was stronger than that of HDC cells. The reason was discussed in detail.
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College of Life Sciences, Peking University, Beijing, China.
Wu
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HL
D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000502:physiology*; D002460:Cell Line; D002678:Chimera; D004586:Electrophoresis; D004624:Embryo Transfer; D007041:Hypoxanthine Phosphoribosyltransferase / Q000172:deficiency; D051379:Mice; D008810:Mice, Inbred C57BL; D008845:Microinjections; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*
D007041:Hypoxanthine Phosphoribosyltransferase