title: Role of pump prime in the etiology and pathogenesis of cardiopulmonary bypass-associated acidosis.
abstract: BACKGROUND
  The development of metabolic acidosis during cardiopulmonary bypass (CPB) is well recognized but poorly understood. The authors hypothesized that the delivery of pump prime fluids is primarily responsible for its development. Accordingly, acid-base changes induced by the establishment of CPB were studied using two types of priming fluid (Haemaccel, a polygeline solution, and Ringer's Injection vs. Plasmalyte 148) using quantitative biophysical methods.
  
  
  METHODS
  A prospective, double-blind, randomized trial was conducted at a tertiary institution with 22 patients undergoing CPB for coronary artery bypass surgery. Sampling of arterial blood was performed at three time intervals: before CPB (t1), 2 min after initiation of CPB at full flows (t2), and at the end of the case (t3). Measurements of Na+, K+, Mg2+, Cl-, HCO3-, phosphate, Ca2+, albumin, lactate, and arterial blood gases at each collection point were performed. Results were analyzed in a quantitative manner.
  
  
  RESULTS
  Immediately on delivery of pump prime fluids, all patients developed a metabolic acidosis (base excess: 0. 95 mEq/l (t1) to -3.65 mEq/l (t2) (P < 0.001) for Haemaccel-Ringer's and 1.17 mEq/l (t1) to -3.20 mEq/l (t2). The decrease in base excess was the same for both primes (-4.60 vs. -4.37; not significant). However, the mechanism of metabolic acidosis was different. With the Haemaccel-Ringer's prime, the metabolic acidosis was hyperchloremic (Delta Cl-, +9.50 mEq/l; confidence interval, 7.00-11.50). With Plasmalyte 148, the acidosis was induced by an increase in unmeasured anions, most probably acetate and gluconate. The resolution of these two processes was different because the excretion of chloride was slower than that of the unmeasured anions (Delta base excess from t1 to t3 = -1.60 for Haemaccel-Ringer's vs. +1.15 for Plasmalyte 148; P = 0.0062).
  
  
  CONCLUSIONS
  Cardiopulmonary bypass-induced metabolic acidosis appears to be iatrogenic in nature and derived from the effect of pump prime fluid on acid-base balance. The extent of such acidosis and its duration varies according to the type of pump prime.
authors.affiliation: Departments of Anaesthesia and Intensive Care, Austin and Repatriation Medical Centre, Melbourne, Australia.
authors.forename: F J
authors.initials: FJ
authors.lastname: Liskaser
authors.forename: R
authors.initials: R
authors.lastname: Bellomo
authors.forename: M
authors.initials: M
authors.lastname: Hayhoe
authors.forename: D
authors.initials: D
authors.lastname: Story
authors.forename: S
authors.initials: S
authors.lastname: Poustie
authors.forename: B
authors.initials: B
authors.lastname: Smith
authors.forename: A
authors.initials: A
authors.lastname: Letis
authors.forename: M
authors.initials: M
authors.lastname: Bennett
mesh_terms: D000136:Acid-Base Equilibrium / Q000187:drug effects; D000138:Acidosis / Q000097:blood / Q000139:chemically induced / Q000209:etiology*; D002315:Cardiopulmonary Bypass / Q000009:adverse effects*; D004311:Double-Blind Method; D006801:Humans; D007552:Isotonic Solutions / Q000009:adverse effects*; D010952:Plasma Substitutes / Q000009:adverse effects*; D011097:Polygeline / Q000009:adverse effects*; D011446:Prospective Studies; D000077331:Ringer's Solution
chemical_list: D007552:Isotonic Solutions; D010952:Plasma Substitutes; D000077331:Ringer's Solution; D011097:Polygeline