title: On-demand electrochemical release of DNA from gold surfaces.
abstract: Electrochemical quartz crystal microbalance (EQCM) is used to probe the electrochemically triggered release of nucleic acids from gold surfaces to solutions of physiological pH. The immobilization of nonthiolated DNA onto the gold surface is followed by an electrostatic desorption at -1.0 V (vs. Ag/AgCl). Steady-state frequency signals, corresponding to the removal of 261- and 644-ng/cm2 single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA), respectively, were attained within 75 and 330 s. As expected for electrostatic repulsion, the amount released can be manipulated by tuning the potential. The small nonsteady-state frequency signals observed at lower potentials indicate promise for a sustained DNA release. Applicability to gold ultramicroelectrodes (12.5-microm radius) is demonstrated in connection with voltammetric blocking experiments. We expect that such on-demand electrochemical release would be a useful addition to the arsenal of nonviral gene delivery routes.
authors.affiliation: Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces 88003, USA. joewang@nmsu.edu
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: M
authors.initials: M
authors.lastname: Jiang
authors.forename: B
authors.initials: B
authors.lastname: Mukherjee
mesh_terms: D004247:DNA / Q000008:administration & dosage / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods*; D018014:Gene Transfer Techniques; D006046:Gold / Q000737:chemistry*; D008839:Microelectrodes
chemical_list: D006046:Gold; D004247:DNA