Screening for antioxidant properties of Salvia reflexa hornem.
The antioxidant properties of the wild growing sage species, Salvia reflexa Hornem., were investigated. The presence of superoxide (O(2)(.-)) and hydroxyl ((.)OH) radicals, malonyldialdehyde (MDA), reduced glutathione (GSH) and total flavonoids were observed in the above-ground parts of plant, as well as activities of the antioxidant enzymes superoxide-dismutase (SOD) and peroxidase (P-ase). The potential antioxidant activity of the methanol:water extract has been assessed based on scavenging activity of stable DPPH free radicals. By the means of TLC and LC/MS a screening for secondary plant products was performed. Significant quantities of O(2)(.-), (.)OH and MDA were observed. Thus, this species exhibited high SOD and P-ase activities as well as a content of total flavonoids. The dominant naturally occurring compound was rosmarinic acid. The results obtained suggested a high antioxidant activity of Salvia reflexa Hornem.
Malencić
D
D
Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovica 8, 21000 Novi Sad, Yugoslavia.
Gasic
O
O
Popović
M
M
Boza
P
P
D000975:Antioxidants / Q000737:chemistry*; D002934:Cinnamates / Q000032:analysis; D053630:Depsides; D016166:Free Radical Scavengers / Q000737:chemistry*; D006801:Humans; D017665:Hydroxyl Radical / Q000032:analysis; D019686:Lamiaceae; D008315:Malondialdehyde / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry; D017382:Reactive Oxygen Species; D013481:Superoxides / Q000032:analysis; D000097123:Rosmarinic Acid
D000975:Antioxidants; D002934:Cinnamates; D053630:Depsides; D016166:Free Radical Scavengers; D017665:Hydroxyl Radical; D008315:Malondialdehyde; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D013481:Superoxides; D000097123:Rosmarinic Acid