title: Isolation and tissue profiles of a large panel of phage antibodies binding to the human adipocyte cell surface.
abstract: Phage display is a powerful technique for the rapid selection and isolation of antibodies to any given target antigen. We have applied this technology to isolate over 100 different human antibodies that bind to antigens expressed in situ on the human adipocyte cell surface. This is a diverse panel of antibodies, as indicated by the V-region sequences. The binding profile of each anti-adipocyte antibody has been characterised using phage antibody immunocytochemistry against a panel of normal human tissues. Although there was some variation in the intensity of the adipocyte staining, each antibody consistently recognised adipocytes, where present, irrespective of the tissue source. In addition, all of the antibodies recognised at least one other cell type other than the adipocyte cell surface. In total, over 50 different tissue-binding profiles were recorded, with the most frequently recognised tissues identified as capillaries or smooth muscle. Extensive tissue binding profiles were generated for some antibodies using a panel of 37 different human tissues. This identified anti-adipocyte antibodies with unexpected profiles, such as FAT.13, which binds only to adipocytes and capillaries in the entire tissue panel. We believe this is the most extensive survey ever undertaken of the human adipocyte cell surface. Moreover, similar methodology could be used to derive complete tissue-binding profiles of antibodies against cell-surface antigens of any cell type. Indeed, by screening antibodies on both normal and diseased tissues, it may be possible to identify antigenic associations between different cell types and the pathologies of many diseases.
authors.affiliation: Cambridge Antibody Technology, The Science Park, Melbourn, SG8 6JJ, Cambridgeshire, UK. bryan.edwards@cambridgeantibody.com
authors.forename: B M
authors.initials: BM
authors.lastname: Edwards
authors.forename: S H
authors.initials: SH
authors.lastname: Main
authors.forename: K L
authors.initials: KL
authors.lastname: Cantone
authors.forename: S D
authors.initials: SD
authors.lastname: Smith
authors.forename: A
authors.initials: A
authors.lastname: Warford
authors.forename: T J
authors.initials: TJ
authors.lastname: Vaughan
mesh_terms: D017667:Adipocytes / Q000276:immunology*; D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D000906:Antibodies / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D000918:Antibody Specificity; D000954:Antigens, Surface / Q000378:metabolism; D002462:Cell Membrane / Q000276:immunology; D022801:Complementarity Determining Regions / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D019151:Peptide Library; D014018:Tissue Distribution
chemical_list: D000906:Antibodies; D000954:Antigens, Surface; D022801:Complementarity Determining Regions; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D019151:Peptide Library