[Expression of prourokinase in different mammalian cells].
Comparison studies of recombinant prourokinase(pro-UK) in various host cells, and expression vectors were carried out. Expression levels of vectors constructed in this study in different cell lines were compared. Mammalian cells expressing pro-UK were established. The levels of pro-UK expression in recombinant Namalwa, Vero and Sp2/0 cells are 200, 12.5 and 50 IU/(10(6) cells 24 h). pro-UK purities separated by immunoaffinity chromatograph are above 90%. Immunoabsorbent assay showed the ratio of pro-UK in CHO cells is lower than that from Vero and Namalwa cells. This study provide new host cells for pro-UK production.
Qian
F
F
Institute of Biotechnology, Beijing.
Xiao
C Z
CZ
Gao
L H
LH
Zhang
Z G
ZG
Guo
Z X
ZX
Yu
W Y
WY
D000818:Animals; D016466:CHO Cells; D002522:Chlorocebus aethiops; D006224:Cricetinae; D004792:Enzyme Precursors / Q000096:biosynthesis*; D008970:Molecular Weight; D011994:Recombinant Proteins / Q000096:biosynthesis*; D014568:Urokinase-Type Plasminogen Activator / Q000096:biosynthesis*; D014709:Vero Cells
D004792:Enzyme Precursors; D011994:Recombinant Proteins; D014568:Urokinase-Type Plasminogen Activator