Duck hepatitis B virus DNA integration induced by oxidative radicals and its transmission from parent cells to offspring ones.
OBJECTIVE
To investigate the role of oxidative radicals in duck hepatitis B virus DNA (DHBV DNA) integration and the transmission of integrated DHBV DNA from parent cells to their offspring cells.


METHODS
TUNEL assay and Southern blot hybridization were used to detect the cellular DNA strand breaks and the incidence of new type of DHBV DNA integration into cellular DNA of LMHD(21-6) cell line after the cells treated by low dose of hydrogen peroxide (10.0 micromol/L) during its growth from one cell to 3 +/- 10(7) to 5 +/- 10(7) cells.


RESULTS
The rate of cell death induced by low dose of H(2)O(2) (10.0 micromol/L) was 32.0%, but that induced by high dose (>10.0 micromol/L) was more than 50.0%. At the same time, the incidence of new DHBV DNA integration into cellular DNA induced by 10.0 micromol/L of H(2)O(2) was 50.0% (6/12) and that in control group was 8.33% (1/12). There was a significant difference between the two groups (P<0.05). Besides, the new integrated DHBV-DNA in the parent cells could be found in the DNA of their offspring cells with the same size of base pair.


CONCLUSIONS
Oxidative radicals is one of the important inducing factors in DHBV-DNA integration into cellular DNA and the integrated DHBV-DNA is able to transmit from the parent cells to their offspring cells stably.
Zhang
L
L
Department of Infectious Diseases, First Affiliated Hospital, Jiangxi Medical College, Nanchang 330006, China.
Rogler
C E
CE
D000818:Animals; D004279:DNA, Viral / Q000032:analysis*; D004372:Ducks; D005609:Free Radicals; D015611:Hepatitis B Virus, Duck / Q000235:genetics*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D016662:Virus Integration / Q000187:drug effects*
D004279:DNA, Viral; D005609:Free Radicals; D006861:Hydrogen Peroxide