Can gastric endoderm change the regionally specific inducing ability of presumptive small intestinal mesoderm?
This study was designed to establish the source of gut mesoderm's ability to induce regional pattern in the endoderm. The most obvious possibility is induction by the endoderm through epithelial-mesenchymal interaction. To test this experimentally, reciprocal quail/chick combinations were prepared of early proventricular endoderm (that is already known to be regionally determined) and presumptive small intestinal mesoderm. The combinations were cultured for 7 days to allow for 'programming' of the mesoderm by the endoderm. After removal of the proventricular endoderm the mesoderm was combined with young gizzard endoderm. It is known that gizzard endoderm can be provoked to develop in either a proventricular or a small intestinal direction by association with the appropriate mesoderm. Thus, by combining intestinal mesoderm 'programmed' by association with proventricular endoderm with gizzard endoderm, the subsequent differentiation of the gizzard endoderm would indicate whether or not the inducing ability of the intestinal mesenchyme had been altered. In addition to such experimental grafts, three types of control graft were prepared. The results of the experiment, based on the morphology of the grafts and the immunocytochemical analysis of selected endocrine cell types, showed that in the majority of cases the gizzard endoderm developed the features of small intestine, not those of proventriculus. This indicates that at the stages studied, endoderm does not act to program mesoderm with which it is associated. If this does occur, it must take place at an earlier stage, i.e., before the time of explantation of the presumptive small intestinal mesoderm (1.25 days of incubation).
Rawdon
B B
BB
Department of Anatomy and Cell Biology, Faculty of Health Sciences, University of Cape Town, South Africa. rawdon@anat.uct.ac.za
D000818:Animals; D019521:Body Patterning; D002642:Chick Embryo; D046508:Culture Techniques; D004064:Digestive System / Q000196:embryology* / Q000378:metabolism; D004707:Endoderm / Q000166:cytology / Q000378:metabolism / Q000637:transplantation; D005768:Gastrointestinal Hormones / Q000378:metabolism; D005895:Gizzard, Avian / Q000196:embryology / Q000378:metabolism; D007150:Immunohistochemistry; D007421:Intestine, Small / Q000196:embryology* / Q000378:metabolism; D008648:Mesoderm / Q000166:cytology / Q000378:metabolism / Q000637:transplantation; D011784:Quail; D014183:Transplantation, Heterologous
D005768:Gastrointestinal Hormones