title: Tilmicosin does not inhibit interleukin-8 gene expression in the bovine lung experimentally infected with Mannheimia (Pasteurella) haemolytica.
abstract: The expression of the interleukin-8 (IL-8) gene was examined by in situ hybridization in lung tissues from calves experimentally infected with Mannheimia (Pasteurella) haemolytica and treated with tilmicosin. Interleukin-8 mRNA expression was detected in alveolar areas, particularly along interlobular septa, in the lumen, and in the epithelial cells of some bronchioles. In lesional lung tissues from animals that had received tilmicosin, we found large areas with limited inflammation. There was no staining for IL-8 mRNA in these areas. In contrast, in strongly inflamed areas, the same patterns and intensities of staining for IL-8 mRNA were detected in tilmicosin- and sham-treated animals. We conclude that tilmicosin does not affect the expression of IL-8 mRNA in tissue showing microscopic signs of inflammation. Together with previous reports, this supports the view that the pro-apoptotic properties of tilmicosin on neutrophils do not compromise the host defense mechanisms required to control the infection.
authors.affiliation: Department of Biological Sciences, University of Calgary, Alberta.
authors.forename: S
authors.initials: S
authors.lastname: Goubau
authors.forename: D W
authors.initials: DW
authors.lastname: Morck
authors.forename: A
authors.initials: A
authors.lastname: Buret
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002417:Cattle / Q000382:microbiology; D002418:Cattle Diseases / Q000188:drug therapy* / Q000382:microbiology; D017403:In Situ Hybridization / Q000662:veterinary; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000235:genetics; D008168:Lung / Q000382:microbiology; D018942:Macrolides; D016978:Mannheimia haemolytica; D009504:Neutrophils / Q000187:drug effects; D010326:Pasteurella Infections / Q000188:drug therapy / Q000662:veterinary*; D015645:Tylosin / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D016209:Interleukin-8; D018942:Macrolides; C052319:tilmicosin; D015645:Tylosin