Intracellular penetration and activity of gemifloxacin in human polymorphonuclear leukocytes.
The intracellular penetration and activity of gemifloxacin in human polymorphonuclear leukocytes (PMN) were evaluated. Gemifloxacin reached intracellular concentrations eight times higher than extracellular concentrations. The uptake was rapid, reversible, and nonsaturable and was affected by environmental temperature, cell viability, and membrane stimuli. At therapeutic extracellular concentrations, gemifloxacin showed intracellular activity against Staphylococcus aureus.
García
I
I
Department of Microbiology, School of Medicine, University of Seville, Seville 41009, Spain.
Pascual
A
A
Ballesta
S
S
Joyanes
P
P
Perea
E J
EJ
D000890:Anti-Infective Agents / Q000378:metabolism* / Q000494:pharmacology; D001692:Biological Transport; D002470:Cell Survival / Q000502:physiology; D024841:Fluoroquinolones; D000077735:Gemifloxacin; D006801:Humans; D009287:Naphthyridines / Q000378:metabolism* / Q000494:pharmacology; D009504:Neutrophils / Q000378:metabolism* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects; D013696:Temperature
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D009287:Naphthyridines; D000077735:Gemifloxacin