[Purification and characteristic of endo-(1--4)-beta-xylanase from Geotrichum candidum 3C].
A method of purification of endo-(1-->4)-beta-xylanase (endoxylanase; EC 3.2.1.8) from the culture liquid of Geotrichum candidum 3C, grown for three days, is described. The enzyme purified 23-fold had a specific activity of 32.6 U per mg protein (yield, 14.4%). Endoxylanase was shown to be homogeneous by SDS-PAGE (molecular weight, 60 to 67 kDa). With carboxymethyl xylan as substrate, the optimum activity (determined viscosimetrically) was recorded at pH 4.0 (pI 3.4). The enzyme retained stability at pH 3.0-4.5 and 30-45 degrees C for 1 h. With xylan from beach wood, the hydrolytic activity of the enzyme (ability to saccharify the substrate) was maximum at 50 degrees C. In 72 h of exposure to 0.2 mg/ml endoxylanase, the extent of saccharification of xylans from birch wood, rye grain, and wheat straw amounted to 10, 12, and 7.7%, respectively. At 0.4 mg/ml, the extent of saccharification of birch wood xylan was as high as 20%. In the case of birch wood xylan, the initial hydrolysis products were xylooligosaccharides with degrees of polymerization in excess of four; the end products were represented by xylobiose, xylotriose, xylose, and acid xylooligosaccharides.
Rodionova
N A
NA
Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, Russia.
Dubovaia
N V
NV
Eneĭskaia
E V
EV
Martinovich
L I
LI
Gracheva
I M
IM
Bezborodov
A M
AM
D043364:Endo-1,4-beta Xylanases; D005656:Fungal Proteins / Q000032:analysis / Q000302:isolation & purification; D005848:Geotrichum / Q000201:enzymology*; D014995:Xylosidases / Q000032:analysis* / Q000302:isolation & purification*
D005656:Fungal Proteins; D014995:Xylosidases; D043364:Endo-1,4-beta Xylanases