Sequence-specific and methylation-dependent and -independent binding of rice nuclear proteins to a rice tungro bacilliform virus vascular bundle expression element.
Nuclear proteins from rice (Oryza sativa) were identified that bind specifically to a rice tungro bacilliform virus promoter region containing a vascular bundle expression element (VBE). One set of proteins of 29, 33, and 37 kDa, present in shoot and cell suspension extracts but hardly detectable in root extracts, bound to a site containing the sequence AGAAGGACCAGA within the VBE, which also contains two CpG and one CpNpG potential methylation motifs. Binding by these proteins was determined to be cytosine methylation-independent. However, a novel protein present in all analyzed extracts bound specifically to the methylated VBE. A region of at least 49 nucleotides overlapping the VBE and complete cytosine methylation of the three Cp(Np)G motifs was required for efficient binding of this methylated VBE-binding protein (MVBP).
He
X
X
Friedrich Miescher Institute, P. O. Box 2543, CH-4002 Basel, Switzerland.
Fütterer
J
J
Hohn
T
T
D044503:5-Methylcytosine / Q000031:analogs & derivatives; D019195:Badnavirus / Q000235:genetics*; D001483:Base Sequence; D018899:CpG Islands; D003596:Cytosine / Q000031:analogs & derivatives*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D012275:Oryza / Q000821:virology*; D010940:Plant Proteins / Q000378:metabolism; D018520:Plant Shoots / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D014018:Tissue Distribution
C065701:5-methyldeoxycytosine; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D010940:Plant Proteins; D044503:5-Methylcytosine; D003596:Cytosine