Efficacy estimates from parasite count data that include zero counts.
Because of the positive skewness of parasite distributions and the greater constancy of percentage of response of therapy in animal populations, parasite count data are conventionally transformed logarithmically before combining results from different animals, either all controls or all treated. Observations of zero counts raise difficulties, since the logarithm of zero is not useful. In this study, several types of zero count adjustments are compared. Two systems for assigning values to zero counts were considered: a fixed system, which assigns the same value to all zero counts regardless of the proportion of such counts in a treatment group, and a variable system, which replaces zero counts with a value based on the proportion of zero counts in the group. The values assigned by either system are then adjusted to reflect aliquot size. An evaluation was performed by using 32 compound Poisson lognormal distributions, three sample sizes, and three representatives of each zero count adjustment system. The Poisson lognormal distribution provides a convenient method with which to provide variability greater than Poisson. Expected values of the sample estimate of the (known) population mean were calculated for each of the 576 combinations of these factors, and the bias associated with each combination was derived. The bias associated with the three representatives of the variable adjustment system was similar. The variable adjustment system had a lower overall bias than any representatives of the fixed adjustment system.
Cox
J L
JL
Pharmaceutical Research and Development, Merial Limited, 2100 Ronson Road, Iselin, New Jersey 08830-3077, USA.
Heyse
J F
JF
Tukey
J W
JW
D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology / Q000627:therapeutic use; D015982:Bias; D010270:Parasite Egg Count / Q000592:standards* / Q000706:statistics & numerical data; D016012:Poisson Distribution; D012756:Sheep; D012757:Sheep Diseases / Q000188:drug therapy / Q000469:parasitology; D014253:Trichostrongylosis / Q000188:drug therapy / Q000469:parasitology / Q000662:veterinary; D014254:Trichostrongylus / Q000187:drug effects / Q000254:growth & development
D000871:Anthelmintics