Effects of vitamin E and prostaglandin E2 on expression of CREB1 and CREB2 proteins by human T lymphocytes.
Both prostaglandins (PGs) and vitamin E are known to deeply affect immune responses. It is shown here that they both influence T cell-mediated immunity through reciprocal interference on the expression of cyclic-AMP responsive element binding (CREB) family proteins. CREB1 protein of human T lymphocytes was significantly modulated by a brief treatment of 5 to 10 min with PGE2. On the contrary, vitamin E appeared to be ineffective on the CREB1 behavior, while it abolished the PGE2-induced modulation of this protein. The CREB2 protein expression was also affected by PGE2 treatment, but a longer period of incubation (>20 min) was needed to observe these changes. Vitamin E showed a strong enhancing effect on CREB2 that was partially reversed by the subsequent treatment with PGE2. Our results support the idea that there is reciprocal interference between PGE2 and vitamin E on PGE2-induced signals in T lymphocytes. These data are in agreement with the reports concerning different cell systems and experimental conditions.
Valenti
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A
Institute of General Pathology, Medical School, University of Messina, Italy.
Venza
I
I
Venza
M
M
Fimiani
V
V
Teti
D
D
D051701:Activating Transcription Factor 4; D000328:Adult; D017362:Cyclic AMP Response Element-Binding Protein; D015232:Dinoprostone / Q000494:pharmacology*; D006801:Humans; D015151:Immunoblotting; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D014157:Transcription Factors / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism*; D014810:Vitamin E / Q000494:pharmacology*
C497109:ATF4 protein, human; C115163:CREB1 protein, human; D017362:Cyclic AMP Response Element-Binding Protein; D014157:Transcription Factors; D014810:Vitamin E; D051701:Activating Transcription Factor 4; D015232:Dinoprostone