title: Radiation effects in lymphocytes of children living in a Chernobyl contaminated region of Belarus.
abstract: OBJECTIVE
  To investigate cytogenetic and mutational effects in lymphocytes from individuals chronically exposed to radiation from the Chernobyl catastrophe.
  
  
  METHODS
  Nine years after the Chernobyl accident (1986), peripheral blood lymphocytes from 20 Kalinkovichi children (age 10-15) and 10 Minsk children (age 10-17) were analysed for genetic damage by several assays. Radiation damage in exposed children was investigated in descendants of progenitor cells that were irradiated during a short period immediately after the accident. In the time-span between the accident and blood sampling the cells were also irradiated chronically by internal radiation originating from ingested radionuclides and, to a smaller extent, by external radiation from radionuclides. The parameters measured in whole blood smears were the frequency of micronucleated mononucleated lymphocytes and binucleated lymphocytes with nucleoplasmic bridges and associated micronuclei. Cultures of cytokinesis-blocked lymphocytes were used to analyse mononuclear and binuclear cells for the presence of micronuclei, also cell killing effects. A colony assay was used to study induction of recessive mutations in the HPRT gene.
  
  
  RESULTS
  The analysis of whole-blood smears indicated a doubling of the frequency of micronuclei per 100 mononuclear lymphocytes in exposed children compared with unirradiated children. Small numbers of binucleated lymphocytes with nucleoplasmic bridges and associated micronuclei were found in blood smears from exposed children. Analysis of cytokinesis-blocked cultures indicated in mononuclear cells of exposed children a statistically significant increase in the frequency of micronuclei. When the same parameters were studied in binucleated cells there was no difference between exposed and unexposed children. Results of the dye-exclusion assay showed a four-fold increase in the percentage of dead cells between exposed and unexposed children. There was no evidence for induction of HPRT mutations in exposed children.
  
  
  CONCLUSIONS
  These results indicate that the frequently advocated procedure of simply analysing micronuclei in cytokinesis-blocked binucleated lymphocytes can result in an underestimate of genetic damage induced by radiation accidents. Biodosimetric studies should therefore employ a battery of assays for the detection of several types of genetic damage in different generations of lymphocytes.
authors.affiliation: Institute of Genetics and Cytology, National Academy of Sciences of Belarus, Minsk. mikhal@biobel.bas-net.by
authors.forename: L S
authors.initials: LS
authors.lastname: Mikhalevich
authors.forename: F A
authors.initials: FA
authors.lastname: De Zwart
authors.forename: G A
authors.initials: GA
authors.lastname: Perepetskaya
authors.forename: N V
authors.initials: NV
authors.lastname: Chebotareva
authors.forename: E A
authors.initials: EA
authors.lastname: Mikhalevich
authors.forename: A D
authors.initials: AD
authors.lastname: Tates
mesh_terms: D000293:Adolescent; D000818:Animals; D002648:Child; D005260:Female; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D008214:Lymphocytes / Q000528:radiation effects* / Q000648:ultrastructure; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000528:radiation effects; D009154:Mutation; D016012:Poisson Distribution; D011210:Power Plants; D018788:Radioactive Hazard Release; D014455:Ukraine
chemical_list: D007041:Hypoxanthine Phosphoribosyltransferase