title: Effect of a bioflavonoid dietary supplement on acetaminophen-induced oxidative injury to feline erythrocytes.
abstract: OBJECTIVE
  To determine the effect of a commercial bioflavonoid antioxidant on acetaminophen-induced oxidative injury to feline erythrocytes.
  
  
  METHODS
  Randomized controlled study.
  
  
  METHODS
  45 healthy age-matched cats.
  
  
  METHODS
  Cats were assigned to 3 experimental groups. Groups 1 and 3 received a bioflavonoid antioxidant (10 mg/d) orally for 2 weeks. Groups 2 and 3 received an oxidative challenge with acetaminophen (90 mg/kg [41 mg/lb] of body weight, PO) on day 7. Packed cell volume, percentage of erythrocytes with Heinz bodies, blood methemoglobin concentration, and blood reduced and oxidized glutathione concentrations were determined at various times during the 2-week study period.
  
  
  RESULTS
  Adverse effects were not associated with bioflavonoid antioxidant administration alone. Acetaminophen administration resulted in a significant increase in methemoglobin concentration in groups 2 and 3; differences were not detected between these groups. Heinz body concentrations in groups 2 and 3 increased after acetaminophen administration; however, the increase in cats that received the antioxidant was significantly less than in group-2 cats. Total blood glutathione concentrations did not change significantly in groups 2 and 3 after acetaminophen administration; however, ratio of reduced to oxidized glutathione concentration increased significantly after administration in group-2 cats, compared with group-3 cats.
  
  
  CONCLUSIONS
  Oral administration of bioflavonoid antioxidants to cats at risk for oxidative stress may have a beneficial effect on their ability to resist oxidative injury to erythrocytes.
authors.affiliation: Department of Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523-1671, USA.
authors.forename: R W
authors.initials: RW
authors.lastname: Allison
authors.forename: E D
authors.initials: ED
authors.lastname: Lassen
authors.forename: M J
authors.initials: MJ
authors.lastname: Burkhard
authors.forename: M R
authors.initials: MR
authors.lastname: Lappin
mesh_terms: D000082:Acetaminophen / Q000009:adverse effects; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D002415:Cats / Q000097:blood*; D019587:Dietary Supplements; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D005419:Flavonoids / Q000008:administration & dosage / Q000494:pharmacology*; D005978:Glutathione / Q000097:blood / Q000187:drug effects; D019803:Glutathione Disulfide / Q000097:blood / Q000187:drug effects; D006366:Heinz Bodies / Q000187:drug effects; D006400:Hematocrit / Q000662:veterinary; D008297:Male; D008706:Methemoglobin / Q000187:drug effects / Q000378:metabolism; D010084:Oxidation-Reduction
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D000082:Acetaminophen; D008706:Methemoglobin; D005978:Glutathione; D019803:Glutathione Disulfide