Expression of rainbow trout growth hormone cDNA in Saccharomyces cerevisiae.
Rainbow trout growth hormone cDNA was modified by polymerase chain reaction (PCR). The modified cDNA was subcloned into the E. coli-yeast shuttle vector pMA91 under the yeast PGK promoter, and transformed into Saccharomyces cerevisiae Y33 to construct an expression strain Y33 (pMArGH16). The recombinant gene could express the growth hormone peptide (about 3% of the total yeast proteins) in Y33 (pMArGH16). The expression product was used as a supplement to feed Tilapia fingerlings. The result showed that the recombinant fish GH could significantly enhance the growth of Tilapia fingerlings.
Jin
M
M
Key Laboratory of Tropical & Subtropical Fish Breeding & Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, CAFS, Guangzhou, China.
Junjie
B
B
Xinhui
L
L
Jianren
L
L
Qing
J
J
Hongjun
Z
Z
D000821:Animal Feed; D000818:Animals; D019587:Dietary Supplements; D005398:Fisheries / Q000379:methods*; D013006:Growth Hormone / Q000096:biosynthesis* / Q000235:genetics; D017686:Oncorhynchus mykiss; D011994:Recombinant Proteins / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae; D017210:Tilapia
D011994:Recombinant Proteins; D013006:Growth Hormone