title: Defect in cell wall integrity of the yeast saccharomyces cerevisiae caused by a mutation of the GDP-mannose pyrophosphorylase gene VIG9.
abstract: The Saccharomyces cerevisiae VIG9 gene encodes GDP-mannose pyrophosphorylase, which synthesizes GDP-mannose from GTP and mannose-1-phosphate. Although the null mutant was lethal, the vig9 mutants so far obtained showed no growth defect but immature protein glycosylation and drug hypersensitivity. During our search for cell-wall mutants, we found a novel temperature-sensitive mutant, JS30, which required an osmotic stabilizer for viability. JS30 excreted cell surface proteins in the medium without any indication of cell lysis. Although conventional genetic analysis using mating was impossible, by detailed characterization of JS30 including an in vitro enzyme assay and nucleotide sequencing, we found the defect of JS30 was due to a mutation in the VIG9 gene. These results indicated a critical role of GDP-mannose in maintenance of cell-wall integrity.
authors.affiliation: Department of Biotechnology, The University of Tokyo, Japan. asdfg@mail.ecc.u-tokyo.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Yoda
authors.forename: T
authors.initials: T
authors.lastname: Kawada
authors.forename: C
authors.initials: C
authors.lastname: Kaibara
authors.forename: A
authors.initials: A
authors.lastname: Fujie
authors.forename: M
authors.initials: M
authors.lastname: Abe
authors.lastname: Hitoshi
authors.lastname: Hashimoto
authors.forename: J
authors.initials: J
authors.lastname: Shimizu
authors.forename: N
authors.initials: N
authors.lastname: Tomishige
authors.forename: Y
authors.initials: Y
authors.lastname: Noda
authors.forename: M
authors.initials: M
authors.lastname: Yamasaki
mesh_terms: D002473:Cell Wall / Q000235:genetics / Q000648:ultrastructure; D004926:Escherichia coli / Q000235:genetics; D017353:Gene Deletion; D007700:Kinetics; D008565:Membrane Proteins / Q000378:metabolism; D009154:Mutation; D009713:Nucleotidyltransferases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000648:ultrastructure*; D013379:Substrate Specificity
chemical_list: D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; D009713:Nucleotidyltransferases; C026917:mannose 1-phosphate guanylyltransferase