[Modification of L-asparaginase with colominic acid and the new characteristics of the modified enzyme].
The colominic acid was covalently coupled to L-asparaginase molecule by reductive amination. Depending on the molar ratios of colominic acid-asparaginase (30:1, 50:1 and 100:1), a modified enzyme molecule contained 4.7, 7.2 and 12 colominic acid molecule, they retained 58%, 56% and 33.2% of the initial asparaginase activity, respectively. In comparison with the native enzyme, modified enzyme had lower immunogenicity and antigenicity, longer half-life time (in vitro), more resistance ability to trypsin proteolysis, and similar Km value for L-asparagine.
Wang
Y D
YD
Department of Enzymology, Institute of Microbiology, Chinese Academy of Sciences, Beijing.
Guo
L
L
Qian
S J
SJ
Meng
G Z
GZ
Zhang
S Z
SZ
D001215:Asparaginase / Q000276:immunology / Q000378:metabolism*; D007700:Kinetics; D008970:Molecular Weight; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*
D011134:Polysaccharides; C014420:colominic acid; D001215:Asparaginase