Demonstration of anti-HuD specific oligoclonal bands in the cerebrospinal fluid from patients with paraneoplastic neurological syndromes. Qualitative evidence of anti-HuD specific IgG-synthesis in the central nervous system.
The presence of HuD-specific oligoclonal IgG bands in the CSF was investigated in five patients with paraneoplastic neurological syndromes. All patients revealed intrathecal synthesis of HuD specific antibodies in the CSF, as estimated from elevated antibody indices (>1.5) in an IgG-ELISA using recombinant HuD-protein as antigen. Isoelectrofocussing combined with affinity blotting showed reactivity of IgG bands with recombinant HuD antigen in all CSF samples. These data support the idea that HuD specific antibodies in the CSF are produced mainly by B-cell clones in the central nervous system. These findings support the hypothesis of autoimmunity in the pathogenesis of anti-Hu associated paraneoplastic neurological syndromes.
Rauer
S
S
Neurologische Klinik und Poliklinik der Albert-Ludwigs-Universität Freiburg, Breisacher Str. 64, D-79106, Freiburg, Germany. rauer@nz11.ukl.uni-freiburg.de
Kaiser
R
R
D000368:Aged; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology; D002490:Central Nervous System / Q000276:immunology; D051959:ELAV Proteins; D000067779:ELAV-Like Protein 4; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000134:cerebrospinal fluid*; D007525:Isoelectric Focusing; D008297:Male; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000276:immunology*; D020364:Paraneoplastic Polyneuropathy / Q000134:cerebrospinal fluid / Q000276:immunology*; D016601:RNA-Binding Proteins / Q000276:immunology*
D051959:ELAV Proteins; D000067779:ELAV-Like Protein 4; C498010:ELAVL4 protein, human; D007074:Immunoglobulin G; D009419:Nerve Tissue Proteins; D016601:RNA-Binding Proteins