Effect of local anesthetics on the bilayer membrane of dipalmitoylphosphatidylcholine: interdigitation of lipid bilayer and vesicle-micelle transition.
The phase transitions of dipalmitoylphosphatidylcholine (DPPC) bilayer membrane were observed by means of differential scanning calorimetry (DSC) as a function of the concentration of local anesthetics, dibucaine (DC x HCl), tetracaine (TC x HCl), lidocaine (LC x HCl) and procaine hydrochlorides (PC x HCl). LC x HCl and PC x HCl depressed monotonously the temperatures of the main- and pre-transition of DPPC bilayer membrane. The enthalpy changes of both transitions decreased slightly with an increase in anesthetic concentration up to 160 mmol kg(-1). In contrast, the addition of TC x HCl or DC x HCl, having the ability to form a micelle by itself, induced the complex phase behavior of DPPC bilayer membrane including the vesicle-to-micelle transition. The depression of both temperatures of the main- and pre-transition, which is accompanied with a decrease in enthalpy, was observed by the addition of TC x HCl up to 21 mmol kg(-1) or DC x HCl up to 11 mmol kg(-1). The pretransition disappeared when these concentrations of anesthetic were added, and the interdigitated gel phase appeared above these concentrations. The appearance of the interdigitated gel phase, instead of the ripple gel phase, brings about the stabilization of the gel phase by 1.8-2.4 kcal mol(-1). In the concentration range of 70-120 mmol kg(-1) TC x HCl (or 40-60 mmol kg(-1) DC x HCl), the enthalpy of the main transition exhibited a drastic decrease, resulting in the virtual disappearance of the main transition. This process includes the decrease in vesicle size with increasing anesthetic concentration, resulting in the mixed micelle of DPPC and anesthetics. Therefore, in this range of anesthetic concentration, the DPPC vesicle solubilized an anesthetic which coexists with the DPPC-anesthetic mixed micelle. Above the concentration of 120 mmol kg(-1) TC x HCl (or 60 mmol kg(-1) DC x HCl), there exists the DPPC-anesthetic mixed micelle. Two types of new transitions concerned with the mixed micelle of DPPC and micelle-forming anesthetics were observed by DSC.
Hata
T
T
Department of Biological Science and Technology, Faculty of Engineering, The University of Tokushima, Minamijosanjima, Japan.
Matsuki
H
H
Kaneshina
S
S
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry / Q000378:metabolism*; D000779:Anesthetics, Local / Q000494:pharmacology*; D002152:Calorimetry, Differential Scanning; D003992:Dibucaine / Q000494:pharmacology; D004553:Electric Conductivity; D008012:Lidocaine / Q000494:pharmacology; D008027:Light; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism*; D008823:Micelles; D015394:Molecular Structure; D010316:Particle Size; D011343:Procaine / Q000494:pharmacology; D012542:Scattering, Radiation; D013696:Temperature; D013816:Thermodynamics
D000779:Anesthetics, Local; D008051:Lipid Bilayers; D008823:Micelles; D015060:1,2-Dipalmitoylphosphatidylcholine; D011343:Procaine; D008012:Lidocaine; D003992:Dibucaine