title: 28-Norcastasterone is biosynthesized from castasterone.
abstract: Metabolic experiments with deuterium-labeled castasterone in seedlings of Arabidopsis thaliana, Oryza saliva and Lycopersicon esculentum, and cultured cells of Catharanthus roseus were performed, and the metabolites were analyzed by GC-MS. In all the plant species examined, [2H3]28-norcastasterone was identified as a metabolite of [26,28-2H6]castasterone, indicating that castasterone is the biosynthetic origin of 28-norcastasterone. Moreover, the natural occurrence of 28-norcastasterone and 28-nortyphasterol in seedlings of A. thaliana has been demonstrated. This is the first report of the natural occurrence of 28-nortyphasterol in plants.
authors.affiliation: RIKEN, The Institute of Physical and Chemical Research, Saitama, Japan. sfujioka@postman.riken.go.jp
authors.forename: S
authors.initials: S
authors.lastname: Fujioka
authors.forename: T
authors.initials: T
authors.lastname: Noguchi
authors.forename: M
authors.initials: M
authors.lastname: Sekimoto
authors.forename: S
authors.initials: S
authors.lastname: Takatsuto
authors.forename: S
authors.initials: S
authors.lastname: Yoshida
mesh_terms: D002478:Cells, Cultured; D002777:Cholestanols / Q000032:analysis / Q000378:metabolism* / Q000494:pharmacology; D019684:Magnoliopsida / Q000166:cytology / Q000378:metabolism; D014316:Tritium
chemical_list: C419985:28-norcastasterone; D002777:Cholestanols; D014316:Tritium; C083545:castasterone