title: Truncation of vertebrate striated muscle myosin light chains disturbs calcium-induced structural transitions in synthetic myosin filaments.
abstract: Electron microscopy and negative staining techniques have been used to show that the proteolytic removal of 13 amino acids from the N-terminus of essential light chain 1 and 19 amino acids from the N-terminus of the regulatory light chain of rabbit skeletal and cardiac muscle myosins destroys Ca(2+)-induced reversible movement of subfragment-2 (S2) with heads (S1) away from the backbone of synthetic myosin filaments observed for control assemblies of the myosin under near physiological conditions. This is the direct demonstration of the contribution of the S2 movement to the Ca(2+)-sensitive structural behavior of rabbit cardiac and skeletal myosin filaments and of the necessity of intact light chains for this movement. In muscle, such a mobility might play an important role in proper functioning of the myosin filaments. The impairment of the Ca(2+)-dependent structural behavior of S2 with S1 on the surface of the synthetic myosin filaments observed by us may be of direct relevance to some cardiomyopathies, which are accompanied by proteolytic breakdown or dissociation of myosin light chains.
authors.affiliation: Institute of Theoretical and Experimental Biophysics RAS, Pushchino, Moscow Region, 142290, Russia. podlubnaya@venus.iteb.serpukhov.su
authors.forename: Z A
authors.initials: ZA
authors.lastname: Podlubnaya
authors.forename: I
authors.initials: I
authors.lastname: Kakol
authors.forename: A
authors.initials: A
authors.lastname: Moczarska
authors.forename: D
authors.initials: D
authors.lastname: Stepkowski
authors.forename: S
authors.initials: S
authors.lastname: Udaltsov
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D018482:Muscle, Skeletal / Q000378:metabolism / Q000648:ultrastructure; D009206:Myocardium / Q000378:metabolism / Q000648:ultrastructure; D018994:Myosin Light Chains / Q000737:chemistry* / Q000378:metabolism* / Q000648:ultrastructure; D015879:Myosin Subfragments / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D011817:Rabbits
chemical_list: D018994:Myosin Light Chains; D015879:Myosin Subfragments; D002118:Calcium