title: Mutation analysis of the growth differentiation factor-9 and -9B genes in patients with premature ovarian failure and polycystic ovary syndrome.
abstract: OBJECTIVE
  To investigate whether growth differentiation factor (GDF)-9 or GDF-9B/bone morphogenetic protein (BMP)-15 mutation is present in Japanese women with premature ovarian failure (POF) and polycystic ovary syndrome (PCOS).
  
  
  METHODS
  Clinical and molecular studies.
  
  
  METHODS
  Outpatient clinic in a university hospital.
  
  
  METHODS
  Fifteen women with POF, 38 women with PCOS, and 3 normal fertile controls.
  
  
  METHODS
  Extraction of DNA from blood samples for subsequent polymerase chain reaction (PCR).
  
  
  METHODS
  Amplified DNA was analyzed by denaturing gradient gel electrophoresis (DGGE) and/or by direct sequencing.
  
  
  RESULTS
  No missense mutation was found in any exons of the GDF-9 gene and the GDF-9B/BMP-15 gene in patients with POF and PCOS.
  
  
  CONCLUSIONS
  The missense mutation in the GDF-9 gene or the GDF-9B/BMP-15 gene is uncommon in anovulatory Japanese women with POF and PCOS.
authors.affiliation: Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Takebayashi
authors.forename: K
authors.initials: K
authors.lastname: Takakura
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.forename: F
authors.initials: F
authors.lastname: Kimura
authors.forename: K
authors.initials: K
authors.lastname: Kasahara
authors.forename: Y
authors.initials: Y
authors.lastname: Noda
mesh_terms: D000328:Adult; D055430:Bone Morphogenetic Protein 15; D004252:DNA Mutational Analysis; D005091:Exons / Q000235:genetics; D005260:Female; D055429:Growth Differentiation Factor 9; D006133:Growth Substances / Q000235:genetics*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D007564:Japan; D020125:Mutation, Missense; D011085:Polycystic Ovary Syndrome / Q000235:genetics*; D016649:Primary Ovarian Insufficiency / Q000235:genetics*; D012016:Reference Values
chemical_list: C525901:BMP15 protein, human; D055430:Bone Morphogenetic Protein 15; C525897:GDF9 protein, human; D055429:Growth Differentiation Factor 9; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins