Nucleotide sequences of the F, L and G protein genes of two non-A/non-B avian pneumoviruses (APV) reveal a novel APV subgroup.
Sequence analysis was performed of all or part of the genes encoding the fusion (F), polymerase (L) and attachment (G) proteins of two French non-A/non-B avian pneumovirus (APV) isolates (Fr/85/1 and Fr/85/2). The two isolates shared at least 99.7% nt and 99.0% aa sequence identity. Comparison with the F genes from subgroup A, subgroup B or Colorado APVs revealed nt and aa identities of 70.0-80. 5% and 77.6-97.2%, respectively, with the L gene sharing 76.1% nt and 85.3% aa identity with that of a subgroup A isolate. The Fr/85/1 and Fr/85/2 G genes comprised 1185 nt, encoding a protein of 389 aa. Common features with subgroup A and subgroup B G proteins included an amino-terminal membrane anchor, a high serine and threonine content, conservation of cysteine residues and a single extracellular region of highly conserved sequence proposed to be the functional domain involved in virus attachment to cellular receptors. However, the Fr/85/1 and Fr/85/2 G sequences shared at best 56.6% nt and 31.2% aa identity with subgroup A and B APVs, whereas these isolates share 38% aa identity. Phylogenetic analysis of the F, G and L genes of pneumoviruses suggested that isolates Fr/85/1 and Fr/85/2 belong to a previously unrecognized APV subgroup, tentatively named D. G-based oligonucleotide primers were defined for the specific molecular identification of subgroup D. These are the first G protein sequences of non-A/non-B APVs to be determined.
Bäyon-Auboyer
Marie-Hélène
MH
Unité de Virologie, Immunologie et Parasitologie Aviaires et Cunicoles1 and Laboratoire de Biologie Moléculaire2, Agence Française de Sécurité Sanitaire des Aliments (AFSSA), BP 53, 22440 Ploufragan, France.
Arnauld
Claire
C
Unité de Virologie, Immunologie et Parasitologie Aviaires et Cunicoles1 and Laboratoire de Biologie Moléculaire2, Agence Française de Sécurité Sanitaire des Aliments (AFSSA), BP 53, 22440 Ploufragan, France.
Toquin
Didier
D
Unité de Virologie, Immunologie et Parasitologie Aviaires et Cunicoles1 and Laboratoire de Biologie Moléculaire2, Agence Française de Sécurité Sanitaire des Aliments (AFSSA), BP 53, 22440 Ploufragan, France.
Eterradossi
Nicolas
N
Unité de Virologie, Immunologie et Parasitologie Aviaires et Cunicoles1 and Laboratoire de Biologie Moléculaire2, Agence Française de Sécurité Sanitaire des Aliments (AFSSA), BP 53, 22440 Ploufragan, France.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001717:Birds / Q000821:virology; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D005814:Genes, Viral; D016679:Genome, Viral; D015707:HN Protein / Q000235:genetics; D008969:Molecular Sequence Data; D018111:Pneumovirus / Q000235:genetics*; D016415:Sequence Alignment; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics*
D015707:HN Protein; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; D014764:Viral Proteins; C116204:attachment protein G; D012321:DNA-Directed RNA Polymerases