title: Similar expression and regulation of Gli2 and Gli3 in the chick limb bud.
abstract: Gli genes encode a family of zinc finger transcription factors that mediate signaling by Hedgehog proteins. We have cloned the chick Gli3 gene and studied its expression in developing chick limbs. Gli3 expression is highly similar to that of chick Gli2. Gli3 mRNA is evenly distributed in the early limb mesenchyme and subsequently downregulated in the posterior mesenchyme by the polarizing activity of Sonic hedgehog. At later stages, Gli3 is expressed in the distal limb mesenchyme.
authors.affiliation: Department of Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.
authors.forename: R
authors.initials: R
authors.lastname: Schweitzer
authors.forename: K J
authors.initials: KJ
authors.lastname: Vogan
authors.forename: C J
authors.initials: CJ
authors.lastname: Tabin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002642:Chick Embryo; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics*; D005121:Extremities / Q000196:embryology*; D018507:Gene Expression Regulation, Developmental; D053823:Hedgehog Proteins; D017403:In Situ Hybridization; D051741:Kruppel-Like Transcription Factors; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D015534:Trans-Activators; D014157:Transcription Factors / Q000235:genetics*; D029867:Xenopus Proteins; D000075746:Zinc Finger Protein Gli2; D000074290:Zinc Finger Protein Gli3; D016335:Zinc Fingers / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; C108003:GLI3 protein, Xenopus; C065683:GLI3 protein, human; C000618551:Gli2 protein, mouse; C465571:Gli3 protein, mouse; D053823:Hedgehog Proteins; D051741:Kruppel-Like Transcription Factors; D009419:Nerve Tissue Proteins; D011506:Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins; C509239:SHH protein, human; D015534:Trans-Activators; D014157:Transcription Factors; D029867:Xenopus Proteins; D000075746:Zinc Finger Protein Gli2; D000074290:Zinc Finger Protein Gli3