title: The immunocytochemical versus cytosol measurement of the oestrogen receptor in invasive breast cancer tissue.
abstract: To compare two methods of measurement of oestrogen receptor (ER)-expression in invasive breast cancer tissue. Sections from 299 breast cancer cases were stained for the ER by immunocytochemical assay (ICA), using mouse monoclonal antibody (MAb) NCL-ER-6SF11, and by the dextran-coated charcoal assay (DCC). Concordant results were observed in 230 of the 299 cases (77%), 69 patients had discordant results (kappa=0.537). We found a moderate concordance between ICA and DCC for ER measurement in breast cancer tissue. If we change the golden standard from DCC to ICA, 23% of patients would receive a different therapeutic approach.
authors.affiliation: Department of Obstet. and Gyn., Algemene Kliniek St-Jan, Broekstraat 104, 1000, Brussel, Belgium.
authors.forename: S L
authors.initials: SL
authors.lastname: Gordts
authors.forename: P
authors.initials: P
authors.lastname: Neven
authors.forename: I
authors.initials: I
authors.lastname: Van Hooff
authors.forename: R
authors.initials: R
authors.lastname: Tonglet
authors.forename: C
authors.initials: C
authors.lastname: Laurent
authors.forename: S
authors.initials: S
authors.lastname: Marechal
authors.forename: X
authors.initials: X
authors.lastname: De Muylder
authors.forename: Y
authors.initials: Y
authors.lastname: Van Belle
authors.forename: G
authors.initials: G
authors.lastname: Vanderick
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D001943:Breast Neoplasms / Q000737:chemistry* / Q000473:pathology; D003600:Cytosol / Q000737:chemistry*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D009361:Neoplasm Invasiveness; D011960:Receptors, Estrogen / Q000032:analysis*
chemical_list: D014408:Biomarkers, Tumor; D011960:Receptors, Estrogen