title: Monoclonal antibody binding to a surface-exposed epitope on Cowdria ruminantium that is conserved among eight strains.
abstract: Monoclonal antibodies (MAb) binding to Cowdria ruminantium elementary bodies (EB) were identified by enzyme-linked immunosorbent assay, and surface binding of one MAb (446.15) to intact EB was determined by immunofluorescence, immunogold labeling, and transmission electron microscopy. MAb 446.15 bound an antigen of approximately 43 kDa in immunoblots of eight geographically distinct strains. The MAb did not react with Ehrlichia canis antigens or uninfected bovine endothelial cell lysate and may be useful in diagnostic assays and vaccine development.
authors.affiliation: Biotechnology and Immunology Laboratory, Kenya Agricultural Research Institute, Kabete, Kenya. shompole@vetmed.wsu.edu
authors.forename: S
authors.initials: S
authors.lastname: Shompole
authors.forename: F R
authors.initials: FR
authors.lastname: Rurangirwa
authors.forename: A
authors.initials: A
authors.lastname: Wambugu
authors.forename: J
authors.initials: J
authors.lastname: Sitienei
authors.forename: D M
authors.initials: DM
authors.lastname: Mwangi
authors.forename: A J
authors.initials: AJ
authors.lastname: Musoke
authors.forename: S
authors.initials: S
authors.lastname: Mahan
authors.forename: C W
authors.initials: CW
authors.lastname: Wells
authors.forename: T C
authors.initials: TC
authors.lastname: McGuire
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000378:metabolism; D000942:Antigens, Bacterial / Q000378:metabolism; D000954:Antigens, Surface / Q000378:metabolism; D001428:Bacterial Vaccines / Q000276:immunology; D002417:Cattle; D016995:Ehrlichia ruminantium / Q000276:immunology* / Q000302:isolation & purification / Q000648:ultrastructure; D000939:Epitopes / Q000378:metabolism; D006357:Heartwater Disease / Q000175:diagnosis / Q000276:immunology; D016253:Microscopy, Immunoelectron; D013045:Species Specificity
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001428:Bacterial Vaccines; D000939:Epitopes