title: Combinatorial signaling in the specification of unique cell fates.
abstract: How multifunctional signals combine to specify unique cell fates during pattern formation is not well understood. Here, we demonstrate that together with the transcription factor Lozenge, the nuclear effectors of the EGFR and Notch signaling pathways directly regulate D-Pax2 transcription in cone cells of the Drosophila eye disc. Moreover, the specificity of D-Pax2 expression can be altered upon genetic manipulation of these inputs. Thus, a relatively small number of temporally and spatially controlled signals received by a set of pluripotent cells can create the unique combinations of activated transcription factors required to regulate target genes and ultimately specify distinct cell fates within this group. We expect that similar mechanisms may specify pattern formation in vertebrate developmental systems that involve intercellular communication.
authors.affiliation: Department of Molecular, Cell, and Developmental Biology and Molecular Biology Institute, University of California at Los Angeles 90095, USA.
authors.forename: G V
authors.initials: GV
authors.lastname: Flores
authors.forename: H
authors.initials: H
authors.lastname: Duan
authors.forename: H
authors.initials: H
authors.lastname: Yan
authors.forename: R
authors.initials: R
authors.lastname: Nagaraj
authors.forename: W
authors.initials: W
authors.lastname: Fu
authors.forename: Y
authors.initials: Y
authors.lastname: Zou
authors.forename: M
authors.initials: M
authors.lastname: Noll
authors.forename: U
authors.initials: U
authors.lastname: Banerjee
mesh_terms: D000818:Animals; D001483:Base Sequence / Q000235:genetics; D019521:Body Patterning / Q000235:genetics*; D002454:Cell Differentiation / Q000235:genetics; D019070:Cell Lineage / Q000235:genetics*; D002999:Clone Cells / Q000166:cytology / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics; D004330:Drosophila / Q000196:embryology* / Q000235:genetics*; D029721:Drosophila Proteins; D004742:Enhancer Elements, Genetic / Q000235:genetics; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism; D005123:Eye / Q000196:embryology* / Q000378:metabolism / Q000648:ultrastructure; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D017344:Genes, Insect / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D051762:PAX2 Transcription Factor; D051880:Receptors, Notch; D017949:Retinal Cone Photoreceptor Cells / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D008565:Membrane Proteins; C497759:N protein, Drosophila; D051762:PAX2 Transcription Factor; D051880:Receptors, Notch; D014157:Transcription Factors; C099964:lz protein, Drosophila; D066246:ErbB Receptors