Formation of disulfide bonds in acid-induced gels of preheated whey protein isolate.
Cold gelation of whey proteins is a two-step process. First, protein aggregates are prepared by a heat treatment of a solution of native proteins in the absence of salt. Second, after cooling of the solution, gelation is induced by lowering the pH at ambient temperature. To demonstrate the additional formation of disulfide bonds during this second step, gelation of whey protein aggregates with and without a thiol-blocking treatment was studied. Modification of reactive thiols on the surface of the aggregates was carried out after the heat-treatment step. To exclude specific effects of the agent itself, different thiol-blocking agents were used. Dynamic light scattering and SDS-agarose gel electrophoresis were used to show that the size of the aggregates was not changed by this modification. The kinetics of gelation as determined by the development of pH and turbidity within the first 8 h of acidification were not affected by blocking thiol groups. During gelation, formation of large, covalently linked, aggregates occurred only in the case of unblocked WPI aggregates, which demonstrates that additional disulfide bonds were formed. Results of permeability and confocal scanning laser microscope measurements did not reveal any differences in the microstructure of networks prepared from treated or untreated whey protein aggregates. However, gel hardness was decreased 10-fold in gels prepared from blocked aggregates. Mixing different amounts of blocked and unblocked aggregates allowed gel hardness to be controlled. It is proposed that the initial microstructure of the gels is primarily determined by the acid-induced noncovalent interactions. The additional covalent disulfide bonds formed during gelation are involved in stabilizing the network and increase gel strength.
Alting
A C
AC
Wageningen Centre for Food Sciences, Wageningen, The Netherlands. alting@nizo.nl
Hamer
R J
RJ
de Kruif
C G
CG
Visschers
R W
RW
D000143:Acids; D004220:Disulfides / Q000138:chemical synthesis*; D004587:Electrophoresis, Agar Gel; D005782:Gels / Q000737:chemistry*; D018613:Microscopy, Confocal; D008894:Milk Proteins / Q000737:chemistry*; D000067816:Whey Proteins
D000143:Acids; D004220:Disulfides; D005782:Gels; D008894:Milk Proteins; D000067816:Whey Proteins