[Vascular endothelial growth gene therapy for narrowing of rabbit iliac artery].
OBJECTIVE
To study the mechanism of renarrowing and vascular endothelial growth factor (VEGF) gene therapy to prevent the restenosis caused by balloon angioplasty.


METHODS
According to the special function of VEGF that promepts the proliferation of endothelial cell (EC), we constituted an eukaryotic expression vector of pcDNA3/VEGF, and added the pcDNA3/VEGF to the surface of polylysine-balloon. We examined the results of the test by in situ blot, eletromagnetic blood flow mechine, scanning eletromicroscope, and light microscope.


RESULTS
There was higher expression of VEGF mRNA and iliac artery intima in the VEGF gene group. The extent of endothelialization was larger, the ratio of intima to media (I/M) was smaller, and the blood flow was more sufficient than in the vector control group.


CONCLUSIONS
VEGF gene local delivery could stimulate the proliferation of EC, accelerate the endothelialization of injury-artery, and inhibite the proliferation of smooth muscle cell (SMC).
Zhang
M
M
Department of Biochemistry and Molecular Biology, Beijing Medical University.
Zhou
A
A
Zhu
G
G
D000800:Angioplasty, Balloon; D000818:Animals; D001157:Arterial Occlusive Diseases / Q000517:prevention & control*; D016228:Endothelial Growth Factors / Q000235:genetics*; D004730:Endothelium, Vascular; D005260:Female; D015316:Genetic Therapy; D007083:Iliac Artery; D008222:Lymphokines / Q000235:genetics*; D008297:Male; D011817:Rabbits; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D016228:Endothelial Growth Factors; D008222:Lymphokines; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors