Human cortactin as putative cancer antigen.
Humoral immune response against overexpressed oncogenic or tumor supressor proteins has been demonstrated for many types of cancer. In this study we report on the detection of the autologous antibody response to putative oncogene, human cortactin using serological analysis of breast carcinoma expression library. Cortactin maps to chromosome 11q13, the region amplified in about 15% of primary breast carcinomas and 30% of head and neck squamous cell carcinomas. Cortactin overexpression due to such amplification might affect adhesive properties of human cancer cells and is associated with poor disease prognosis. Accordingly, we detected overexpression of cortactin transcript in autologous tumor and amplification/overexpression of cortactin in tumors of breast cancer patients serologically positive for this marker. We demonstrate that 15% of breast cancer patients elicit humoral immune response against human cortactin.
Lagarkova
M A
MA
Laboratory of Molecular Immunology, Belozersky Institute of Physico-Chemical Biology, Center for Molecular Medicine, Moscow State University, Moscow, 119899, Russia.
Boitchenko
V E
VE
Mescheryakov
A A
AA
Kashkarova
U A
UA
Nedospasov
S A
SA
D000595:Amino Acid Sequence; D000912:Antibodies, Neoplasm / Q000097:blood; D000951:Antigens, Neoplasm / Q000235:genetics / Q000276:immunology*; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics / Q000276:immunology*; D002277:Carcinoma / Q000235:genetics; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D051356:Cortactin; D005260:Female; D015723:Gene Library; D006801:Humans; D008840:Microfilament Proteins / Q000235:genetics / Q000276:immunology*; D008875:Middle Aged; D008969:Molecular Sequence Data
D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; C495868:CTTN protein, human; D051356:Cortactin; D008840:Microfilament Proteins