title: [Multiple types of cytokines secreted by a mouse fetal liver stromal cell line].
abstract: A murine fetal liver stromal cell line, called MFLC, has been established and maintained in DME supplemented with 10%NCS for 2 years. Without exogenous stimulation, the MFLC cells spontaneously secreted several types of cytokines, including IL-6, GM-CSF and chemotactic factor. Of which, IL-6 and chemotactic factor were abundant, GM-CSF at low level. Biological activities of IL-7 and IL-3 were not detected in the MFLC cell's supernatants. MFLC-SN induced colony formation of murine bone marrow cells in a dose dependent fashion in which mixed granulocyte/macrophage/megakaryocyte colonies(CFU-GMM) and granulocyte/macrophage colonies(CFU-GM) were dominant. MFLC-SN also sustained colony formation of bone marrow cells taken from 5-Fu injected mice, suggesting that there was a biological activity similar to SCF in MFLC-SN. The cytokines secreted by MFLC might play an important role in T cell early development in fetal liver. These results will be useful for us to analyse the mechanism of T cell development in fetal liver and to study the cytokine network regulation.
authors.affiliation: Department of Immunology, Beijing Medical University.
authors.forename: Q
authors.initials: Q
authors.lastname: Liu
authors.forename: Z G
authors.initials: ZG
authors.lastname: Liu
authors.forename: W F
authors.initials: WF
authors.lastname: Chen
authors.forename: P X
authors.initials: PX
authors.lastname: Zhang
authors.forename: X W
authors.initials: XW
authors.lastname: Pang
authors.forename: X P
authors.initials: XP
authors.lastname: Qian
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002460:Cell Line; D002630:Chemotactic Factors / Q000378:metabolism; D003114:Colony-Forming Units Assay; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D005333:Fetus; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D017154:Stromal Cells / Q000378:metabolism
chemical_list: D002630:Chemotactic Factors; D016207:Cytokines; D015850:Interleukin-6; D016178:Granulocyte-Macrophage Colony-Stimulating Factor