Acute rejection-associated tubular basement membrane defects and chronic allograft nephropathy.
BACKGROUND
Acute rejection is a major risk factor for chronic allograft nephropathy, although the link(s) between these events is not understood. The hypothesis of this study is that alterations in tubular basement membranes (TBMs) that occur during acute rejection may be irreversible and thereby play a role in the development of chronic allograft nephropathy.


METHODS
Fourteen renal transplant patients were selected, each having had two or more biopsies performed (42 total). All biopsies were scored for acute and chronic rejection using Banff 1997 criteria. The initial biopsy showed only acute interstitial rejection (type I rejection). No biopsies contained significant chronic arterial lesions of chronic vascular rejection. The entire cortex was examined on Jones methenamine silver-stained sections at x400 for interruption in TBM staining. The number of tubules with TBM abnormalities was counted, and the renal cortical area was measured by image analysis. Periodic acid-Schiff/immunoperoxidase stain was performed on 12 acute rejection biopsies stained for laminin, cytokeratin 7, CD3, CD20, and CD68. Controls consisted of 11 biopsies (8 negative for rejection and 3 acute tubular necrosis).


RESULTS
Numerous TBM alterations in silver staining were identified as being associated with acute rejection and tubulitis, consisting of abrupt TBM discontinuities and/or extreme attenuation with segmental or complete absence of TBM. A loss of TBM matrix proteins was confirmed by absent laminin staining in areas of acute rejection and tubulitis. There was herniation of tubular cells into the interstitium through TBM defects confirmed by cytokeratin staining. The TBM defects were spatially associated with inflammatory cells, particularly macrophages. When the biopsies were divided into two groups, <10 and> 10 TBM breaks/mm2, there were statistically significant morphologic and clinical correlations. The number of TBM disruptions correlated with the serum creatinine at the time of biopsy, a combined Banff t + i score, the difference in tubular atrophy between the initial and most recent biopsy and the difference between the nadir creatinine and most recent creatinine.


CONCLUSIONS
Damage to TBM develops in acute rejection as a consequence of interstitial inflammation and tubulitis. These lytic events correlate with the later development of clinical and morphologic evidence of chronic injury in the absence of arterial injury of chronic rejection. We suggest that chronic allograft nephropathy may have an inflammatory interstitial origin.
Bonsib
S M
SM
Department of Pathology, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA.
Abul-Ezz
S R
SR
Ahmad
I
I
Young
S M
SM
Ellis
E N
EN
Schneider
D L
DL
Walker
P D
PD
D000208:Acute Disease; D001485:Basement Membrane / Q000473:pathology; D002908:Chronic Disease; D006084:Graft Rejection / Q000150:complications* / Q000473:pathology*; D006801:Humans; D007674:Kidney Diseases / Q000209:etiology*; D016030:Kidney Transplantation; D007684:Kidney Tubules / Q000473:pathology*; D009393:Nephritis / Q000209:etiology / Q000473:pathology; D011183:Postoperative Complications / Q000209:etiology*; D014184:Transplantation, Homologous