title: Anesthetic concentrations of riluzole inhibit neuronal nitric oxide synthase activity, but not expression, in the rat hippocampus.
abstract: We hypothesized that anesthetic dose of riluzole, an inhibitor of glutamate neurotransmission, may affect the activity and/or expression of neuronal NOS (nNOS). Riluzole, N(G)-nitro-L-arginine-methyl ester (L-NAME) and 7-nitro indazole (7-NI) produced a concentration-related inhibition of nNOS activity in vitro. Riluzole competed with 7-NI for inhibition of nNOS activity, but had no effect on nNOS or endothelial NOS (eNOS) protein expression. Also, nNOS activity was significantly decreased in riluzole-anesthetized rats (40 mg kg(-1) i.p., -32+/-6% from controls, P<0.05). Therefore, blockade of nNOS activity may be involved in the anesthetic effects of riluzole in vivo.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale (INSERM) U 408, Faculté de Médecine Xavier Bichat, 16 rue Henri Huchard, 75018, Paris, France.
authors.forename: H
authors.initials: H
authors.lastname: Keita
authors.forename: J
authors.initials: J
authors.lastname: Boczkowski
authors.forename: A
authors.initials: A
authors.lastname: Samb
authors.forename: S
authors.initials: S
authors.lastname: Lanone
authors.forename: L
authors.initials: L
authors.lastname: Lang-Lazdunski
authors.forename: D
authors.initials: D
authors.lastname: Rouellé
authors.forename: J M
authors.initials: JM
authors.lastname: Desmonts
authors.forename: J
authors.initials: J
authors.lastname: Mantz
mesh_terms: D000199:Actins / Q000187:drug effects / Q000378:metabolism; D000777:Anesthetics / Q000494:pharmacology*; D000818:Animals; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D007191:Indazoles / Q000494:pharmacology; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000187:drug effects* / Q000378:metabolism; D052248:Nitric Oxide Synthase Type I; D052250:Nitric Oxide Synthase Type III; D051381:Rats; D017207:Rats, Sprague-Dawley; D019782:Riluzole / Q000494:pharmacology*
chemical_list: D000199:Actins; D000777:Anesthetics; D004791:Enzyme Inhibitors; D007191:Indazoles; D019782:Riluzole; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; D052250:Nitric Oxide Synthase Type III; C496322:Nos1 protein, rat; C496316:Nos3 protein, rat; C080122:7-nitroindazole; D019331:NG-Nitroarginine Methyl Ester