Gene therapy for established murine collagen-induced arthritis by local and systemic adenovirus-mediated delivery of interleukin-4.
To determine whether IL-4 is therapeutic in treating established experimental arthritis, a recombinant adenovirus carrying the gene that encodes murine IL-4 (Ad-mIL-4) was used for periarticular injection into the ankle joints into mice with established collagen-induced arthritis (CIA). Periarticular injection of Ad-mIL-4 resulted in a reduction in the severity of arthritis and joint swelling compared with saline- and adenoviral control groups. Local expression of IL-4 also reduced macroscopic signs of joint inflammation and bone erosion. Moreover, injection of Ad-mIL-4 into the hind ankle joints resulted in a decrease in disease severity in the untreated front paws. Systemic delivery of murine IL-4 by intravenous injection of Ad-mIL-4 resulted in a significant reduction in the severity of early-stage arthritis.
Kim
S H
SH
Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA.
Evans
C H
CH
Kim
S
S
Oligino
T
T
Ghivizzani
S C
SC
Robbins
P D
PD
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D001168:Arthritis / Q000139:chemically induced / Q000276:immunology / Q000628:therapy*; D004195:Disease Models, Animal; D018450:Disease Progression; D005786:Gene Expression Regulation; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000627:therapeutic use; D016753:Interleukin-10 / Q000235:genetics; D015847:Interleukin-4 / Q000235:genetics / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA
D016753:Interleukin-10; D015847:Interleukin-4