title: Deficiency mapping of quantitative trait loci affecting longevity in Drosophila melanogaster.
abstract: In a previous study, sex-specific quantitative trait loci (QTL) affecting adult longevity were mapped by linkage to polymorphic roo transposable element markers, in a population of recombinant inbred lines derived from the Oregon and 2b strains of Drosophila melanogaster. Two life span QTL were each located on chromosomes 2 and 3, within sections 33E-46C and 65D-85F on the cytological map, respectively. We used quantitative deficiency complementation mapping to further resolve the locations of life span QTL within these regions. The Oregon and 2b strains were each crossed to 47 deficiencies spanning cytological regions 32F-44E and 64C-76B, and quantitative failure of the QTL alleles to complement the deficiencies was assessed. We initially detected a minimum of five and four QTL in the chromosome 2 and 3 regions, respectively, illustrating that multiple linked factors contribute to each QTL detected by recombination mapping. The QTL locations inferred from deficiency mapping did not generally correspond to those of candidate genes affecting oxidative and thermal stress or glucose metabolism. The chromosome 2 QTL in the 35B-E region was further resolved to a minimum of three tightly linked QTL, containing six genetically defined loci, 24 genes, and predicted genes that are positional candidates corresponding to life span QTL. This region was also associated with quantitative variation in life span in a sample of 10 genotypes collected from nature. Quantitative deficiency complementation is an efficient method for fine-scale QTL mapping in Drosophila and can be further improved by controlling the background genotype of the strains to be tested.
authors.affiliation: Department of Genetics, College of Agriculture and Life Sciences, North Carolina State University, Raleigh, North Carolina 27695, USA.
authors.forename: E G
authors.initials: EG
authors.lastname: Pasyukova
authors.forename: C
authors.initials: C
authors.lastname: Vieira
authors.forename: T F
authors.initials: TF
authors.lastname: Mackay
mesh_terms: D000818:Animals; D002869:Chromosome Aberrations; D002874:Chromosome Mapping; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development; D005260:Female; D017353:Gene Deletion; D005816:Genetic Complementation Test; D005819:Genetic Markers; D007178:Inbreeding; D008136:Longevity / Q000235:genetics*; D008297:Male; D015233:Models, Statistical; D019655:Quantitative Trait, Heritable; D011995:Recombination, Genetic
chemical_list: D005819:Genetic Markers