Cell cycle-related expression and ligand binding of peripheral benzodiazepine receptor in human breast cancer cell lines.
The aim of this study was to measure the expression of peripheral benzodiazepine receptors (PBR) as well as mitochondria content in different phases of the cell cycle of BT-20 and MCF-7 breast cancer cell lines, using two-parameter flow cytometric analyses. The PBR expression as well as mitochondria mass, were found to increase as cells pass through different stages of the cell cycle, whereas the amount of PBR in quiescent cells was very low. Binding capacity for the PBR ligand [3H]-Ro5-4864 was strongly related to the phase of the cell cycle with a positive correlation (r=0.98) with a high percentage of cells in S phase. Incubation of BT-20 cells in serum-deprived medium with nanomolar concentrations of Ro5-4864 caused an increase in S phase cells. This effect was not observed in MCF-7 cells. Using micromolar concentrations of Ro5-4864, both BT-20 and MCF-7 cells were reversibly arrested in the G(0/1) phase.
Sänger
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Department of Gynecology and Obstetrics, Johann Wolfgang Goethe University, Theodor-Stern Kai 7, 60590, Frankfurt, Germany.
Strohmeier
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Kaufmann
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Kuhl
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H
D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D002453:Cell Cycle; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008928:Mitochondria / Q000378:metabolism; D011963:Receptors, GABA-A / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D011963:Receptors, GABA-A