title: Systemic hypothermia following spinal cord compression injury in the rat: an immunohistochemical study on MAP 2 with special reference to dendrite changes.
abstract: Systemic hypothermia has been shown to exert neuroprotective effects in experimental ischemic CNS models caused by vascular occlusions. The present study addresses the question as to whether systemic hypothermia has similar neuroprotective qualities following severe spinal cord compression trauma using microtubule-associated protein 2 (MAP2) immunohistochemistry combined with the avidin-biotin-peroxidase complex method as marker to identify neuronal and dendritic lesions. Fifteen rats were randomized into three equally sized groups. One group sustained thoracic laminectomy, the others severe spinal cord compression trauma of the T8-9 segment. The control group contained laminectomized animals submitted to a hypothermic procedure in which the esophageal temperature was reduced from 38 degrees C to 30 degrees C. The two trauma groups were either submitted to the same hypothermic procedure or kept normothermic during the corresponding time. All animals were sacrificed 24 h following the surgical procedure. The MAP2 immunostaining in the normothermic trauma group indicated marked reductions in MAP2 antigen in the cranial and caudal peri-injury zones (T7 and T10, respectively). This reduction was much less pronounced in the hypothermic trauma group. In fact, the MAP2 antigen was present in almost equally sized areas in both the hypothermic groups independent of previous laminectomy alone or the addition of trauma. Our study thus indicates that hypothermia has a neuroprotective effect on dendrites of rat spinal cords subjected to compression trauma.
authors.affiliation: Department of Genetics and Pathology, University Hospital, Uppsala, Sweden. Yngve.Olsson@genpat.uu.se
authors.forename: W R
authors.initials: WR
authors.lastname: Yu
authors.forename: H
authors.initials: H
authors.lastname: Westergren
authors.forename: M
authors.initials: M
authors.lastname: Farooque
authors.forename: A
authors.initials: A
authors.lastname: Holtz
authors.forename: Y
authors.initials: Y
authors.lastname: Olsson
mesh_terms: D000818:Animals; D001831:Body Temperature; D003712:Dendrites / Q000473:pathology; D007035:Hypothermia / Q000209:etiology* / Q000503:physiopathology; D007150:Immunohistochemistry; D008297:Male; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000473:pathology; D013117:Spinal Cord Compression / Q000150:complications* / Q000378:metabolism / Q000473:pathology
chemical_list: D008869:Microtubule-Associated Proteins