Flavonol glycosides from Aristeguietia discolor reduce morphine withdrawal in vitro.
The effects of extracts, partially purified fractions and four flavonol glycosides 1-4 from Aristeguietia discolor were investigated on the naloxone-precipitated withdrawal contraction of the acute morphine dependent guinea-pig ileum in vitro. After a 4 min in vitro exposure to morphine a strong contraction of guinea-pig isolated ileum was observed after the addition of naloxone. Both MeOH extract (50, 100 and 200 mg/mL), the partially purified fractions I, L, M and N (50, 100 and 200 mg/mL) and flavonol glycosides 1-4 (1 x 10(-4) 5 x 10(-5) 1 x 10(-5) M), injected 10 min before morphine, were capable of blocking the naloxone-induced contraction after exposure to morphine in a concentration-dependent fashion. The results of the present paper suggest that flavonol glycosides from Aristeguietia discolor may play an important role in the control of morphine withdrawal.
Capasso
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Dipartimento di Scienze Farmaceutiche, Università di Salerno, Italy. annacap@unisa.it
Saturnino
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Simone
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Aquino
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D000818:Animals; D019659:Asteraceae; D004195:Disease Models, Animal; D005419:Flavonoids / Q000494:pharmacology* / Q000627:therapeutic use; D044948:Flavonols; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects; D008297:Male; D008519:Medicine, Traditional; D009020:Morphine / Q000009:adverse effects* / Q000037:antagonists & inhibitors; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects; D009270:Naloxone / Q000633:toxicity; D009292:Narcotic Antagonists / Q000494:pharmacology* / Q000627:therapeutic use; D010568:Peru; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D013375:Substance Withdrawal Syndrome / Q000517:prevention & control*
D005419:Flavonoids; D044948:Flavonols; D009292:Narcotic Antagonists; D010936:Plant Extracts; D009270:Naloxone; D009020:Morphine