title: Cell-free synthesis of poliovirus: 14S subunits are the key intermediates in the encapsidation of poliovirus RNA.
abstract: In a cell-free system of uninfected HeLa cells, programmed with poliovirus RNA, extraneous radiolabelled 14S subunits assembled with endogenous 14S subunits and interacted with newly synthesized RNA to form virions (160S). This result suggests that 14S subunits are the key intermediates in the encapsidation of poliovirus RNA.
authors.affiliation: Department of Microbiology and Hygiene, Vrije Universiteit Brussel, Laarbeeklaan 103, B-1090 Brussels, Belgium1.
authors.forename: Yvan
authors.initials: Y
authors.lastname: Verlinden
authors.affiliation: Department of Molecular Genetics and Microbiology, School of Medicine, State University of New York at Stony Brook, Stony Brook, NY 11794, USA2.
authors.forename: Andrea
authors.initials: A
authors.lastname: Cuconati
authors.affiliation: Department of Molecular Genetics and Microbiology, School of Medicine, State University of New York at Stony Brook, Stony Brook, NY 11794, USA2.
authors.forename: Eckard
authors.initials: E
authors.lastname: Wimmer
authors.affiliation: Department of Microbiology and Hygiene, Vrije Universiteit Brussel, Laarbeeklaan 103, B-1090 Brussels, Belgium1.
authors.forename: Bart
authors.initials: B
authors.lastname: Rombaut
mesh_terms: D002474:Cell-Free System; D017955:Poliovirus; D012367:RNA, Viral; D019065:Virus Assembly
chemical_list: D012367:RNA, Viral