title: Comparison of different liquid chromatography methods for the determination of corticosteroids in biological matrices.
abstract: Various extraction techniques can be combined with column liquid chromatography (LC) and ultraviolet (UV) or mass spectrometric (MS) detection for the determination of synthetic corticosteroids in biological matrices. Target analysis of low concentrations of 25 microg/kg of dexamethasone in feed can be performed by combining immunoaffinity chromatography (IAC) and LC with UV detection. A straightforward multi-analyte procedure is obtained by tandem solid-phase extraction (SPE) and subsequent LC-UV. However, the limits of detection for feed samples are then relatively poor, viz. 100 microg/kg. A multi-analyte method which meets modern demands of about 5 microg/kg detection limit requires one-step SPE combined with LC-MS analysis. As regards urine corticosteroids can be determined down to a level of 0.5 microg/l by either SPE-LC-MS- MS or SPE(IAC)-LC-MS.
authors.affiliation: National Institute of Public Health and the Environment (RIVM), Laboratory for Residue Analysis, Bilthoven, Netherlands.
authors.lastname: Stolke AAM
authors.lastname: Schwillens PLWJ
authors.forename: L A
authors.initials: LA
authors.lastname: van Ginkel
authors.forename: U A
authors.initials: UA
authors.lastname: Brinkman
mesh_terms: D000305:Adrenal Cortex Hormones / Q000032:analysis* / Q000652:urine; D000821:Animal Feed / Q000032:analysis; D002846:Chromatography, Affinity / Q000379:methods*; D013058:Mass Spectrometry; D012680:Sensitivity and Specificity
chemical_list: D000305:Adrenal Cortex Hormones