title: Thymocytes express the golli products of the myelin basic protein gene and levels of expression are stage dependent.
abstract: The golli products of the myelin basic protein gene have been shown to be expressed in mouse thymus and brain. The full repertoire of thymic cell types expressing golli products has not yet been determined, although immunoreactivity has been found in some macrophages. We have analyzed the cellular expression of golli mRNAs and proteins in the thymus. The results showed that MTS5(+) cortical/MTS10(+) medullary epithelial cells and NLDC145(+) dendritic cells did not express golli, while some macrophages did exhibit strong immunoreactivity. GOLLI: mRNAs were not detected in macrophages by in situ hybridization. Thymocytes expressed significant levels of golli mRNAs and proteins by in situ hybridization and immunohistochemistry. Interestingly, golli immunoreactivity varied with thymocyte stage of differentiation. For example, CD4(-)CD8(-) (double-negative) thymocytes expressed relatively high levels of golli. Upon further differentiation into CD4(-)CD8(-) (double-positive) thymocytes, golli protein expression declined dramatically. When thymocytes developed into CD8(-) or CD4(+) (single-positive) thymocytes, golli protein expression increased again, but it never achieved the levels found in double-negative thymocytes. Thus, the altered levels of expression of golli proteins in developing thymocytes correlated with the transitions from double-negative to double-positive and double-positive to single-positive stages. The lack of significant golli expression in thymic stromal cells may offer an alternative explanation for the mechanism of inefficient negative selection of those autoreactive thymocytes with specificity for myelin basic proteins.
authors.affiliation: Developmental Biology Group, Neuropsychiatric Institute, and Brain Research Institute, University of California Medical School, Los Angeles, CA 90024, USA.
authors.forename: J M
authors.initials: JM
authors.lastname: Feng
authors.forename: I M
authors.initials: IM
authors.lastname: Givogri
authors.forename: E R
authors.initials: ER
authors.lastname: Bongarzone
authors.forename: C
authors.initials: C
authors.lastname: Campagnoni
authors.forename: E
authors.initials: E
authors.lastname: Jacobs
authors.forename: V W
authors.initials: VW
authors.lastname: Handley
authors.forename: V
authors.initials: V
authors.lastname: Schonmann
authors.forename: A T
authors.initials: AT
authors.lastname: Campagnoni
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D002467:Cell Nucleus / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D003593:Cytoplasm / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D005786:Gene Expression Regulation / Q000276:immunology*; D007108:Immune Tolerance; D007150:Immunohistochemistry; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D004676:Myelin Basic Protein / Q000096:biosynthesis* / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis; D017154:Stromal Cells / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology / Q000378:metabolism*
chemical_list: D004676:Myelin Basic Protein; D012333:RNA, Messenger