title: Use of the Strep-Tag and streptavidin for detection and purification of recombinant proteins.
authors.affiliation: Lehrstuhl für Biologische Chemie, Technische Universität München, Freising-Weihenstephan, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Skerra
authors.forename: T G
authors.initials: TG
authors.lastname: Schmidt
mesh_terms: D000345:Affinity Labels; D000469:Alkaline Phosphatase / Q000235:genetics; D000595:Amino Acid Sequence; D019677:Artificial Gene Fusion / Q000379:methods; D017123:Bacteriophage T7 / Q000201:enzymology / Q000235:genetics; D053148:Caspase 3; D020169:Caspases / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D002846:Chromatography, Affinity / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003001:Cloning, Molecular / Q000379:methods; D012321:DNA-Directed RNA Polymerases / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development; D015951:Factor Xa; D006801:Humans; D008969:Molecular Sequence Data; D009842:Oligopeptides; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000032:analysis* / Q000302:isolation & purification; D015183:Restriction Mapping; D019809:Streptavidin
chemical_list: D000345:Affinity Labels; C099517:Ala-Trp-Arg-His-Pro-Gln-Phe-Gly-Gly; D009842:Oligopeptides; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D019809:Streptavidin; D012321:DNA-Directed RNA Polymerases; D000469:Alkaline Phosphatase; D015951:Factor Xa; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases