Ser162-Dependent inactivation of overproduced sucrose-phosphate synthase protein of maize leaf in transgenic rice plants.
To investigate the role of Ser162 in phosphorylation-dependent regulation of maize sucrose-phosphate synthase (SPS) activities in rice, transgenic rice plants expressing wild-type or mutagenized maize SPS were produced. Our results indicate that Ser162 was responsible for overproduction-induced inactivation of SPS protein and for light/ dark modulation of this protein in vivo.
Takahashi
S
S
Department of Plant Physiology, National Institute of Agrobiological Resources, Kannondai, Tsukuba, Iharaki, Japan. sakiko@ss.abr.go.jp
Ono
K
K
Ugaki
M
M
Ishimaru
K
K
Aoki
N
N
Ohsugi
R
R
D015971:Gene Expression Regulation, Enzymologic; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism*; D006601:Hexoses / Q000032:analysis; D016297:Mutagenesis, Site-Directed; D012275:Oryza; D010766:Phosphorylation; D018515:Plant Leaves / Q000737:chemistry / Q000201:enzymology*; D030821:Plants, Genetically Modified; D012694:Serine; D013213:Starch / Q000032:analysis; D013395:Sucrose / Q000032:analysis; D003313:Zea mays
D006601:Hexoses; D012694:Serine; D013395:Sucrose; D013213:Starch; D005964:Glucosyltransferases; C022698:sucrose-phosphate synthase