title: Oxidative stress in the latissimus dorsi muscle of diabetic rats.
abstract: The purpose of the present study was to investigate the effects of experimental diabetes on the oxidant and antioxidant status of latissimus dorsi (LD) muscles of male Wistar rats (220 +/- 5 g, N = 11). Short-term (5 days) diabetes was induced by a single injection of streptozotocin (STZ, 50 mg/kg, iv; glycemia >300 mg/dl). LD muscle of STZ-diabetic rats presented higher levels of thiobarbituric acid reactive substances (TBARS) and chemiluminescence (0.36 +/- 0.02 nmol/mg protein and 14706 +/- 1581 cps/mg protein) than LD muscle of normal rats (0.23 +/- 0.04 nmol/mg protein and 7389 +/- 1355 cps/mg protein). Diabetes induced a 92% increase in catalase and a 27% increase in glutathione S-transferase activities in LD muscle. Glutathione peroxidase activity was reduced (58%) in STZ-diabetic rats and superoxide dismutase activity was similar in LD muscle of both groups. A positive correlation was obtained between catalase activity and the oxidative stress of LD, as evaluated in terms of TBARS (r = 0.78) and by chemiluminescence (r = 0.89). Catalase activity also correlated inversely with glutathione peroxidase activity (r = 0.79). These data suggest that an increased oxidative stress in LD muscle of diabetic rats may be related to skeletal muscle myopathy.
authors.affiliation: Laboratório de Fisiologia Cardiovascular, Departamento de Fisiologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.
authors.forename: K L
authors.initials: KL
authors.lastname: De Angelis
authors.forename: I A
authors.initials: IA
authors.lastname: Cestari
authors.forename: J
authors.initials: J
authors.lastname: Barp
authors.forename: P
authors.initials: P
authors.lastname: Dall'Ago
authors.forename: T G
authors.initials: TG
authors.lastname: Fernandes
authors.forename: P I
authors.initials: PI
authors.lastname: de Bittencourt
authors.forename: A
authors.initials: A
authors.lastname: Belló-Klein
authors.forename: A A
authors.initials: AA
authors.lastname: Belló
authors.forename: S
authors.initials: S
authors.lastname: Llesuy
authors.forename: M C
authors.initials: MC
authors.lastname: Irigoyen
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D016014:Linear Models; D008163:Luminescent Measurements; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D017392:Thiobarbituric Acid Reactive Substances