title: Replication of wild-type and mutant human cytomegalovirus in life-extended human diploid fibroblasts.
abstract: A cDNA encoding the catalytic subunit of human telomerase was used to generate life-extended derivatives of primary human diploid fibroblasts. The life-extended cells supported efficient human cytomegalovirus (HCMV) replication. A subclone of the life-extended cells was generated containing the HCMV UL82 gene and used to isolate and propagate a virus that exhibited a profound growth defect after infection at a low input multiplicity.
authors.affiliation: Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544-1014, USA. wbresnahan@molbio.princeton.edu
authors.forename: W A
authors.initials: WA
authors.lastname: Bresnahan
authors.forename: G E
authors.initials: GE
authors.lastname: Hultman
authors.forename: T
authors.initials: T
authors.lastname: Shenk
mesh_terms: D003587:Cytomegalovirus / Q000235:genetics* / Q000502:physiology*; D005347:Fibroblasts / Q000502:physiology* / Q000821:virology*; D006801:Humans; D009154:Mutation; D019098:Telomerase / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D014779:Virus Replication
chemical_list: D014764:Viral Proteins; C051298:cytomegalovirus phosphoprotein 71kDa; D019098:Telomerase