Involvement of hrs binding protein in IgE receptor-triggered exocytosis in RBL-2H3 mast cells.
Hrs binding protein (Hbp) tightly associated with Hrs is thought to play a regulatory role in vesicular trafficking during endocytosis and exocytosis. In this study, we have expressed dominant-negative mutants of Hbp to evaluate their effects on the degranulation of secretory granules in RBL-2H3 mast cells. The dominant-negative mutants of Hbp significantly inhibited IgE receptor (FcepsilonRI)-triggered secretory response as tested by beta-hexosaminidase release. These results suggest that Hbp functions as a regulator in the FcepsilonRI-triggered degranulation of secretory granules in mast cells.
Murai
S
S
Department of Biological Sciences, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama, 226-8501, Japan.
Kitamura
N
N
D016475:3T3 Cells; D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D015550:Cell Degranulation / Q000235:genetics; D056827:Endosomal Sorting Complexes Required for Transport; D005089:Exocytosis / Q000235:genetics / Q000502:physiology*; D017353:Gene Deletion; D008407:Mast Cells / Q000378:metabolism* / Q000502:physiology; D051379:Mice; D010750:Phosphoproteins / Q000096:biosynthesis; D051381:Rats; D017455:Receptors, IgE / Q000502:physiology*; D014407:Tumor Cells, Cultured
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D056827:Endosomal Sorting Complexes Required for Transport; D010750:Phosphoproteins; D017455:Receptors, IgE; C491648:Stam protein, mouse; C491646:Stam2 protein, mouse; C096164:hepatocyte growth factor-regulated tyrosine kinase substrate