Entamoeba histolytica: rapid detection of indian isolates by cysteine proteinase gene-specific polymerase chain reaction.
Amoebiasis, caused by Entamoeba histolytica, is still one of the major problems for developing countries like India. Early detection of the parasite is a must for its prevention and control. In this study, PCR analysis of the cysteine proteinase gene from clinical isolates of symptomatic intestinal and amoebic liver abscess (ALA) cases has been compared with the stool microscopy, serology, and ultrasonography methods. The clinical isolates negative for E. histolytica by stool microscopy demonstrated the presence of the cysteine proteinase gene by PCR amplification. Also the gene copy number was increased in ALA samples compared with intestinal cases. Hence an accurate, early, and easier detection was possible by cysteine proteinase gene amplification directly from the clinical samples.
Prakash
A
A
Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Chakraborti
A
A
Mahajan
R C
RC
Ganguly
N K
NK
D000328:Adult; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D003546:Cysteine Endopeptidases / Q000235:genetics*; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D004404:Dysentery, Amebic / Q000175:diagnosis / Q000469:parasitology*; D004748:Entamoeba histolytica / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D005243:Feces / Q000469:parasitology; D005260:Female; D006386:Hemagglutination Tests; D006801:Humans; D007194:India; D007223:Infant; D008101:Liver Abscess, Amebic / Q000175:diagnosis / Q000469:parasitology*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D014774:Virulence
D000913:Antibodies, Protozoan; D016054:DNA, Protozoan; D003546:Cysteine Endopeptidases