Monoclonal antibody binding to a surface-exposed epitope on Cowdria ruminantium that is conserved among eight strains.
Monoclonal antibodies (MAb) binding to Cowdria ruminantium elementary bodies (EB) were identified by enzyme-linked immunosorbent assay, and surface binding of one MAb (446.15) to intact EB was determined by immunofluorescence, immunogold labeling, and transmission electron microscopy. MAb 446.15 bound an antigen of approximately 43 kDa in immunoblots of eight geographically distinct strains. The MAb did not react with Ehrlichia canis antigens or uninfected bovine endothelial cell lysate and may be useful in diagnostic assays and vaccine development.
Shompole
S
S
Biotechnology and Immunology Laboratory, Kenya Agricultural Research Institute, Kabete, Kenya. shompole@vetmed.wsu.edu
Rurangirwa
F R
FR
Wambugu
A
A
Sitienei
J
J
Mwangi
D M
DM
Musoke
A J
AJ
Mahan
S
S
Wells
C W
CW
McGuire
T C
TC
D000818:Animals; D000907:Antibodies, Bacterial / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000378:metabolism; D000942:Antigens, Bacterial / Q000378:metabolism; D000954:Antigens, Surface / Q000378:metabolism; D001428:Bacterial Vaccines / Q000276:immunology; D002417:Cattle; D016995:Ehrlichia ruminantium / Q000276:immunology* / Q000302:isolation & purification / Q000648:ultrastructure; D000939:Epitopes / Q000378:metabolism; D006357:Heartwater Disease / Q000175:diagnosis / Q000276:immunology; D016253:Microscopy, Immunoelectron; D013045:Species Specificity
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001428:Bacterial Vaccines; D000939:Epitopes