Two conserved lysines at the 50/20-kDa junction of myosin are necessary for triggering actin activation.
Actin stimulates myosin's activity by inducing structural alterations that correlate with the transition from a weakly to a strongly bound state, during which time inorganic phosphate (P(i)) is released from myosin's active site. The surface loop at the 50/20-kDa junction of myosin (loop 2) is part of the actin interface. Here we demonstrate that elimination of two highly conserved lysines at the C-terminal end of loop 2 specifically blocks the ability of heavy meromyosin to undergo a weak to strong binding transition with actin in the presence of ATP. Removal of these lysines has no effect on strong binding in the absence of nucleotide, on the rate of ADP binding or release, or on the basal ATPase activity. We further show that the 16 amino acids of loop 2 preceding the lysine-rich region are not essential for actin activation, although they do modulate myosin's affinity for actin in the presence of ATP. We conclude that interaction of the conserved lysines with acidic residues in subdomain 1 of actin either triggers a structural change or stabilizes a conformation that is necessary for actin-activated release of P(i) and completion of the ATPase cycle.
Joel
P B
PB
Department of Molecular Physiology and Biophysics, University of Vermont College of Medicine, Burlington, Vermont 05405-0068, USA.
Trybus
K M
KM
Sweeney
H L
HL
D000199:Actins / Q000378:metabolism*; D000244:Adenosine Diphosphate / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002645:Chickens; D017124:Conserved Sequence; D066298:In Vitro Techniques; D008239:Lysine / Q000235:genetics / Q000378:metabolism*; D008970:Molecular Weight; D009154:Mutation; D015879:Myosin Subfragments / Q000378:metabolism / Q000502:physiology; D009218:Myosins / Q000235:genetics / Q000378:metabolism*; D011492:Protein Hydrolysates / Q000378:metabolism; D014364:Tryptophan / Q000737:chemistry / Q000378:metabolism
D000199:Actins; D015879:Myosin Subfragments; D011492:Protein Hydrolysates; C007978:hydrolysin; D000244:Adenosine Diphosphate; D014364:Tryptophan; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; D009218:Myosins; D008239:Lysine