title: Quantitative in vitro bioassay for recombinant human interleukin-11.
abstract: A cell culture-based in vitro bioassay was developed to measure the biological activity of recombinant human interleukin-11 (rhIL-11). The bioassay measures induced proliferation of T10 cells, derived from the T1165 murine plasmacytoma line. A colorimetrically detectable formazan product, obtained by cellular reduction of the tetrazolium compound, 4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate (WST-1), was used as an endpoint for response of clone T10 to added rhIL-11. Positions of the samples and the standards in 96-well microplates affected the precision of this bioassay, which was improved by using 2 microplates where serially diluted sample and standard lines were interleaved and their positions were alternated. The coefficient of variation for this bioassay was less than 8%. This method is suitable for quality control of rhIL-11 because of its simplicity, reproducibility, and accuracy.
authors.affiliation: Yamanouchi Pharmaceutical Co., Ltd, Tsukuba-shi, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yokota
authors.forename: M
authors.initials: M
authors.lastname: Kishimoto
authors.forename: H
authors.initials: H
authors.lastname: Saito
authors.forename: T
authors.initials: T
authors.lastname: Sakai
authors.forename: S
authors.initials: S
authors.lastname: Yokota
authors.forename: S
authors.initials: S
authors.lastname: Kojima
authors.forename: Y
authors.initials: Y
authors.lastname: Taniguchi
authors.forename: H
authors.initials: H
authors.lastname: Kaniwa
authors.forename: N
authors.initials: N
authors.lastname: Saisho
mesh_terms: D000818:Animals; D001681:Biological Assay; D002138:Calibration; D002460:Cell Line; D003124:Colorimetry; D006801:Humans; D006825:Hybridomas; D007202:Indicators and Reagents; D017370:Interleukin-11 / Q000032:analysis*; D051379:Mice; D011786:Quality Control; D011994:Recombinant Proteins / Q000032:analysis; D015203:Reproducibility of Results
chemical_list: D007202:Indicators and Reagents; D017370:Interleukin-11; D011994:Recombinant Proteins