title: Continuous enteral nutrition attenuates pulmonary edema in rats exposed to 100% oxygen.
abstract: Adult rats exposed to hyperoxia develop anorexia, weight loss, and a lung injury characterized by pulmonary edema and decreased lung liquid clearance. We hypothesized that maintenance of nutrition during hyperoxia could attenuate hyperoxia-induced pulmonary edema. To test this hypothesis, we enterally fed adult male Sprague-Dawley rats via gastrostomy tubes and exposed them to oxygen (inspired O(2) fraction >0.95) for 64 h. In contrast to controls, enterally fed hyperoxic animals did not lose weight and had smaller pleural effusions and wet-to-dry weight ratios (a measure of lung edema) that were not different from room air controls. Enterally fed rats exposed to hyperoxia had increased levels of mRNA for the Na(+)-K(+)-ATPase alpha(1)- and beta(1)-subunits and glutathione peroxidase. These findings suggest that maintenance of nutrition during an oxidative lung injury reduces lung edema, perhaps by allowing for continued expression and function of protective proteins such as the Na(+)-K(+)-ATPase.
authors.affiliation: Pulmonary and Critical Care Medicine, Evanston Northwestern Healthcare, Evanston 60201, Illinois, USA. pfactor@Northwestern.edu
authors.forename: P
authors.initials: P
authors.lastname: Factor
authors.forename: K
authors.initials: K
authors.lastname: Ridge
authors.forename: J
authors.initials: J
authors.lastname: Alverdy
authors.forename: J I
authors.initials: JI
authors.lastname: Sznajder
mesh_terms: D000818:Animals; D004750:Enteral Nutrition; D005774:Gastrostomy; D015971:Gene Expression Regulation, Enzymologic; D005979:Glutathione Peroxidase / Q000378:metabolism; D018496:Hyperoxia / Q000139:chemically induced / Q000178:diet therapy / Q000378:metabolism; D008168:Lung / Q000201:enzymology; D008297:Male; D010100:Oxygen / Q000633:toxicity*; D010996:Pleural Effusion / Q000139:chemically induced / Q000178:diet therapy / Q000378:metabolism; D011654:Pulmonary Edema / Q000139:chemically induced / Q000178:diet therapy* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics; D014556:Urine; D014867:Water / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D014867:Water; D005979:Glutathione Peroxidase; D000254:Sodium-Potassium-Exchanging ATPase; D010100:Oxygen