[The diversity of human hematopoietic stem/progenitor cells: VIII. Sterological features of CD 34+ hematopoietic cells isolated from human bone marrow].
Human CD 34+ hematopoietic cells, a distinctive cell population containing hematopoietic stem/progenitor cells, have the capability to highly self-renewal, differentiation into all lineages of committed progenitor cells and reconstitution of both long-term hematopoiesis and immune functions after transplantation. In our previous study, the morphological, ultrastructural and cytochemical features concerning CD 34+ hematopoietic cells obtained by a two-step isolated systems of CIMS-100/FACS 440 with 100% purity were depicted. Based on these observations on CD 34+ hematopoietic cells under light microscope, SEM and TEM, we recently analyze the sterological features of CD 34+ hematopoietic cells with the aid of Quantimet 970 automatic image analyser so that the three-dimension structures regarding CD 34+ hematopoietic cells could be further made clear. By a series of measures including image scan-->modulus transform-->shadow correction-->image store-->statistical analysis, some morphometric parameters of CD 34+ hematopoietic cells were obtained as the followings: diametrs 3.490-6.741 microns, perimeters 11.776-26.240 microns, surface area 9.565-35.686 microns2, form factors 1.048-1.840, nucleus-plasma ratio 0.55-0.72, mean light density 0.17675-0.65100, integral light density 2717.217-46661.000. These morphometric data combined with our previous results from morphology, ultrastructure and functional subsets of CD 34+ hematopoietic cells strongly demonstrate that CD 34+ hematopoietic cells are really a heterogenous population. The possible reasons causing heterogeneous are close associated with either different functional subsets or differentiation lineages. To our knowledge, this is the first identification of sterological features of CD 34+ hematopoietic cells.
Xi
Y Z
YZ
Department of Transplantation Immunology, Affiliated 307 Hospital for Academy of Medical Sciences, Beijing.
Zhang
Z X
ZX
Zhang
S X
SX
Kong
F H
FH
Tang
P X
PX
Wei
W
W
D018952:Antigens, CD34 / Q000032:analysis*; D001854:Bone Marrow Cells / Q000648:ultrastructure*; D006412:Hematopoietic Stem Cells / Q000737:chemistry / Q000648:ultrastructure*; D006801:Humans; D008855:Microscopy, Electron, Scanning
D018952:Antigens, CD34