Sensitivity of a S. cerevisiae RAD27 deletion mutant to DNA-damaging agents and in vivo complementation by the human FEN-1 gene.
We have investigated the sensitivity to DNA-damaging agents of a strain of Saccharomyces cerevisiae containing a deletion of the RAD27 gene. The mutant strain is sensitive to a number of alkylating agents that modify DNA at a variety of positions, including one that produces primarily phosphotriesters. In contrast, the mutant strain is not sensitive to the oxidizing agent hydrogen peroxide. The introduction of a plasmid containing the FEN-1 gene (the human ortholog of the RAD27 gene) can substantially complement the sensitivity to alkylating agents observed in the mutant strain.
Hansen
R J
RJ
Department of Biology, College of St. Benedict/St. John's University, Collegeville, MN 56321-3000, USA.
Friedberg
E C
EC
Reagan
M S
MS
D001483:Base Sequence; D004249:DNA Damage; D017931:DNA Primers; D004706:Endodeoxyribonucleases / Q000235:genetics*; D045585:Flap Endonucleases; D005816:Genetic Complementation Test; D006801:Humans; D008770:Methylnitrosourea / Q000633:toxicity*; D009153:Mutagens / Q000494:pharmacology*; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins
D017931:DNA Primers; D009153:Mutagens; D029701:Saccharomyces cerevisiae Proteins; D008770:Methylnitrosourea; D004706:Endodeoxyribonucleases; D045585:Flap Endonucleases; C468317:FEN1 protein, human; C468323:RAD27 protein, S cerevisiae