Adaptation of post-traumatic angiogenesis in the rabbit knee by apposition of torn ligament ends.
Apposition of torn ligament ends has been shown to have a beneficial effect on healing of the medial collateral ligament; however, the mechanism underlying this improved recovery is unclear. Excessive post-traumatic angiogenesis, an inherent component of soft-tissue regeneration, may be functionally detrimental in relatively hypovascular tissues such as ligaments. The present study therefore examined the relationship between contact of transected ligament ends and vascular remodeling. Female New Zealand White rabbits were subjected to a gap injury, Z-plasty apposition, or sham operation to the midsubstance of the medial collateral ligament. Six weeks after treatment, the volume of vessels supplying the healing zone of the medial collateral ligament, as well as the ipsilateral lateral collateral ligament, posterior cruciate ligament. menisci, and medial capsule, was quantified by carmine red vascular casting. The volume of vessels supplying the neoligamentous scar formed by gap injury to the medial collateral ligament was found to be twice that of ligaments that had undergone the sham operation, and lateral collateral ligament and meniscal vascularity was also augmented in the injured joint. The medial collateral ligaments that underwent Z-plasty apposition exhibited a level of vascularity comparable with that of the control ligaments that had undergone the sham procedure, whereas meniscal and lateral collateral ligament vascularities remained elevated in this group. Capsular and posterior cruciate ligament vascularities were unaffected by gap injury or Z-plasty to the ipsilateral medial collateral ligament. These findings indicate that injury to the medial collateral ligament not only stimulates angiogenesis in the healing ligament, but other ipsilateral soft tissues also undergo vascular remodeling. Furthermore, apposition of an injured medial collateral ligament modifies these pro-angiogenic events, and this may partly explain why contact of torn ligament ends is beneficial for post-traumatic recovery of an injured joint.
McDougall
J J
JJ
McCaig Centre for Joint Injury and Arthritis Research, University of Calgary, Alberta, Canada. mcdougaj@ucalgary.ca
Yeung
G
G
Leonard
C A
CA
Sutherland
C
C
Bray
R C
RC
D000222:Adaptation, Physiological / Q000502:physiology*; D000818:Animals; D001178:Arthroplasty; D002329:Carmine; D002921:Cicatrix / Q000378:metabolism; D004396:Coloring Agents; D005260:Female; D007150:Immunohistochemistry; D007718:Knee Injuries / Q000503:physiopathology* / Q000601:surgery; D007719:Knee Joint / Q000098:blood supply / Q000378:metabolism / Q000503:physiopathology; D017888:Medial Collateral Ligament, Knee / Q000098:blood supply* / Q000293:injuries* / Q000502:physiology; D018919:Neovascularization, Physiologic / Q000502:physiology*; D011817:Rabbits; D018180:Thrombomodulin / Q000032:analysis; D014867:Water / Q000378:metabolism
D004396:Coloring Agents; D018180:Thrombomodulin; D014867:Water; D002329:Carmine