title: The value of pyrolysis mass spectrometry to investigate nosocomial outbreaks caused by Serratia marcescens.
abstract: Simultaneous outbreaks of S. marcescens infection going on in the Neonatal Intensive Care Unit and the Surgical Department of the same hospital were investigated by pyrolysis mass spectrometry (PyMS). The PyMS analysis of the strains clearly demonstrated that the two outbreaks were caused by different strains. The 14 S. marcescens isolates from the first outbreak were closely related, with the exception of one environmental isolate, which did not harbour the ESBL plasmid, which was present in all other isolates. However, the phage type of all 14 isolates was the same. Among the 9 S. marcescens isolates from the second outbreak, PyMS clearly distinguished 3 that exhibited gentamicin resistance from the remaining 6 gentamicin-susceptible isolates. Phage typing was unhelpful in this case, as none of the isolates were typable. The PyMS typing of nosocomial outbreak strains can reach the level of discrimination approaching that achieved by molecular genetic analysis.
authors.affiliation: Department of Clinical Microbiology, Albert Szent-Györgyi Medical University, Szeged, Hungary.
authors.forename: E
authors.initials: E
authors.lastname: Nagy
authors.forename: Z
authors.initials: Z
authors.lastname: Koczián
authors.forename: Z
authors.initials: Z
authors.lastname: Prágai
authors.forename: P R
authors.initials: PR
authors.lastname: Sisson
authors.forename: R
authors.initials: R
authors.lastname: Freeman
mesh_terms: D015373:Bacterial Typing Techniques; D003428:Cross Infection / Q000382:microbiology*; D004196:Disease Outbreaks; D006801:Humans; D007231:Infant, Newborn; D013058:Mass Spectrometry / Q000379:methods*; D008826:Microbial Sensitivity Tests; D016868:Serratia Infections / Q000382:microbiology*; D012706:Serratia marcescens / Q000145:classification*