Investigating the genetic control of stem cell behavior.
The precise control of hematopoietic stem cell fate decisions involves molecules that are differentially expressed in the stem cell versus its non-stem progeny. The construction of representative and high quality cDNA libraries containing stem cell-specific sequences is a first step in elucidating stem cell control mechanisms. Automated bioinformatics in conjunction with high-throughput random sequencing and high-density parallel array hybridization studies make it possible to dissect stem cell molecular pathways and networks. It is the differential analysis of these interacting pathways which will provide the greatest insights into the biological differences underlying stem cell behavior.
Phillips
R L
RL
Department of Molecular Biology, Princeton University, New Jersey 08544, USA.
D019295:Computational Biology / Q000379:methods; D016208:Databases, Factual; D020869:Gene Expression Profiling; D015723:Gene Library; D020411:Oligonucleotide Array Sequence Analysis; D017422:Sequence Analysis, DNA; D013234:Stem Cells