Quantitation of Pseudomonas aeruginosa in wound biopsy samples: from bacterial culture to rapid 'real-time' polymerase chain reaction.
STATEMENT OF FINDINGS: We developed a real-time detection (RTD) polymerase chain reaction (PCR) with rapid thermal cycling to detect and quantify Pseudomonas aeruginosa in wound biopsy samples. This method produced a linear quantitative detection range of 7 logs, with a lower detection limit of 103 colony-forming units (CFU)/g tissue or a few copies per reaction. The time from sample collection to result was less than 1h. RTD-PCR has potential for rapid quantitative detection of pathogens in critical care patients, enabling early and individualized treatment.
Pirnay
J P
JP
Queen Astrid Military Hospital, Neder-Over-Heembeek, Belgium. skinbank@hmra.smd.be
De Vos
D
D
Duinslaeger
L
L
Reper
P
P
Vandenvelde
C
C
Cornelis
P
P
Vanderkelen
A
A
D015373:Bacterial Typing Techniques / Q000191:economics / Q000379:methods*; D001706:Biopsy; D002056:Burns / Q000150:complications*; D015169:Colony Count, Microbial; D003428:Cross Infection / Q000209:etiology / Q000382:microbiology* / Q000473:pathology; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000191:economics / Q000379:methods*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods*; D011552:Pseudomonas Infections / Q000209:etiology / Q000382:microbiology* / Q000473:pathology; D011550:Pseudomonas aeruginosa / Q000145:classification* / Q000235:genetics; D012680:Sensitivity and Specificity; D013997:Time Factors; D014946:Wound Infection / Q000209:etiology / Q000382:microbiology* / Q000473:pathology
D004269:DNA, Bacterial