Reversed-phase separation of achiral isomers by varying temperature and either gradient time or solvent strength.
The separation of several isomer pairs of widely varied structure was studied as a function of changes in temperature, gradient time, mobile phase pH, column type ("monomeric" vs. "polymeric" C18-silica) and organic solvent (methanol vs. acetonitrile). General conclusions are drawn which may prove useful in future attempts at the separation of these and other achiral isomers.
Snyder
L R
LR
LC Resources Inc., Orinda, CA 94563, USA.
Dolan
J W
JW
D012997:Solvents; D013237:Stereoisomerism; D013696:Temperature
D012997:Solvents