title: Distribution of NADPH-diaphorase reactivity and effects of nitric oxide on feeding and locomotory circuitry in the pteropod mollusc, Clione limacina.
abstract: The action of nitric oxide (NO) and the distribution of putative nitric oxide synthase-containing cells in the pelagic pteropod mollusc Clione limacina were studied using nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) histochemistry and conventional microelectrode techniques in the isolated central nervous system and in semi-intact preparations. The majority of NADPH-d-reactive neuronal somata were restricted to the cerebral ganglia. The labeled cells were small in diameter (20-30 microm) and were located in the medial areas of the ganglia. A pair of symmetrical neurons was found in the peripheral "olfactory organ." NADPH-d-reactive non-neuronal cells were detected in the periphery and were mainly associated with secretorylike cells and organs of the renopericardial system. The NO donor, diethylamine NO complex sodium salt (10-100 microM), activated neurons from both feeding and locomotory circuits. The cGMP analog, 8-Br-cGMP, mimicked the effects of NO on neurons. We suggest that NO is an endogenous neuromodulator involved in the control of some aspects of feeding and locomotor behavior of Clione.
authors.affiliation: The Whitney Laboratory, Department of Neuroscience, University of Florida, St. Augustine, Florida 32080-8623, USA. moroz@whitney.ufl.edu
authors.forename: L L
authors.initials: LL
authors.lastname: Moroz
authors.forename: T P
authors.initials: TP
authors.lastname: Norekian
authors.forename: T J
authors.initials: TJ
authors.lastname: Pirtle
authors.forename: K J
authors.initials: KJ
authors.lastname: Robertson
authors.forename: R A
authors.initials: RA
authors.lastname: Satterlie
mesh_terms: D000818:Animals; D002490:Central Nervous System / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005247:Feeding Behavior / Q000187:drug effects* / Q000502:physiology; D017952:Ganglia, Invertebrate / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008124:Locomotion / Q000187:drug effects* / Q000502:physiology; D008974:Mollusca / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D009415:Nerve Net / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D014781:Viscera / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
chemical_list: D020030:Nitric Oxide Donors; D009569:Nitric Oxide; D009252:NADPH Dehydrogenase