Rapid-cycle PCR for detection and typing of Mycoplasma pneumoniae in clinical specimens.
We designed several new primers and modified previously described species- and type-specific primers targeting the Mycoplasma pneumoniae P1 adhesin gene. Optimized thermal profiles allowed one-step or nested PCR to be completed in less than 1 h. In 10 patients with pneumonia, M. pneumoniae type 1 was identified in 3 and type 2 in 7.
Kong
F
F
Centre for Infectious Diseases and Microbiology, Institute of Clinical Pathology and Medical Research, Westmead Hospital, Westmead, New South Wales 2145, Australia.
Gordon
S
S
Gilbert
G L
GL
D018829:Adhesins, Bacterial / Q000235:genetics; D015373:Bacterial Typing Techniques / Q000379:methods; D002648:Child; D017931:DNA Primers; D006801:Humans; D009177:Mycoplasma pneumoniae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D011019:Pneumonia, Mycoplasma / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity
D018829:Adhesins, Bacterial; D017931:DNA Primers