title: The transduction of the signal for grape bud dormancy breaking induced by hydrogen cyanamide may involve the SNF-like protein kinase GDBRPK.
abstract: Alterations in gene expression during early stages of dormancy release in grapevine buds were analyzed to facilitate the identification of gene products that may mediate the signal transduction of a dormancy-release signal, or derepression of meristematic activity. In the present report we describe the identification of GDBRPK, a transcript for an SNF-like protein kinase that is up-regulated upon chemical induction of dormancy release by hydrogen cyanamide (HC). Since SNF and SNF-like protein kinases are known as sensors of stress signals, we hypothesize that GDBRPK may be involved in the perception of a stress signal induced by HC. We also describe a simultaneous and remarkable induction of both PDC and ADH transcripts that was observed shortly after HC application, and was of a transient nature. These data may imply that HC application leads to a transient respiratory stress, which likely results in a temporary increase in the AMP/ATP ratio. Since AMP is known as a stress signal that is sensed by SNF-like kinases, we suggest that the SNF-like GDBRPK could serve as the sensor of this signal.
authors.affiliation: Department of Tree Breeding, Institute of Horticulture, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel. vhettior@agri.gov.il
authors.forename: E
authors.initials: E
authors.lastname: Or
authors.forename: I
authors.initials: I
authors.lastname: Vilozny
authors.forename: Y
authors.initials: Y
authors.lastname: Eyal
authors.forename: A
authors.initials: A
authors.lastname: Ogrodovitch
mesh_terms: D000426:Alcohol Dehydrogenase / Q000187:drug effects / Q000378:metabolism; D000595:Amino Acid Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003484:Cyanamide / Q000494:pharmacology*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D005285:Fermentation / Q000187:drug effects; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D008969:Molecular Sequence Data; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D011767:Pyruvate Decarboxylase / Q000187:drug effects / Q000378:metabolism; D018749:RNA, Plant / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D019685:Rosales / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D018749:RNA, Plant; D003484:Cyanamide; D000426:Alcohol Dehydrogenase; D017346:Protein Serine-Threonine Kinases; D011767:Pyruvate Decarboxylase