Transcriptional analysis of inducible acetamidase gene of Mycobacterium smegmatis.
Isolation and analysis of strong and regulatable promoters of mycobacteria should be useful tools to aid the expression of cloned genes in mycobacteria. In the present study, we have mapped the transcriptional start site of the inducible acetamidase gene of Mycobacterium smegmatis and studied the mechanism of its regulation. Northern blot, reverse transcription-PCR and primer extension analysis studies were used to determine the position of the promoter.
Narayanan
S
S
Department of Immunology, Tuberculosis Research Centre, Mayor V.R. Ramanathan Road, Chennai 600 031, Chetput, India.
Selvakumar
S
S
Aarati
R
R
Vasan
S K
SK
Narayanan
P R
PR
D000581:Amidohydrolases / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D015152:Blotting, Northern; D017931:DNA Primers; D005821:Genetic Techniques; D020102:Mycobacterium smegmatis / Q000201:enzymology / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012329:RNA, Bacterial / Q000032:analysis / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
D017931:DNA Primers; D012329:RNA, Bacterial; D012333:RNA, Messenger; D000581:Amidohydrolases; C021445:acetamidase