[Studies on meiotic delay and aneuploidies induced by colcemid (COM) in male mouse germ cells].
Colcemid (COM) was tested for induction of meiotic delay and aneuploidies in meiotic metaphase II (MMII) of male(101/E 1 XC 3 H/E 1)F 1 mice post single intraperitoneum (i.p.) injection. The dose of 1 mg/kg of COM was used and sampled at 2, 6, 10, 14 and 18 h after COM treatment. The number of MMII and MMI were the highest at 2 and 6 h repectively, and decreased rapidly with lengthening of COM treatment and reached the lowest at 14 h; then increased. However, the ratio of MMII to MMI was always significantly higher than in solvent control at every sample interval. Under our experimental conditions, COM did not show aneugenicity in male mouse germ cells. The possible mechanisms by which COM caused meiotic delay and reasons that COM did not induce aneuploidy in MMII were discussed.
Shi
Q H
QH
Nanjing Normal University.
Adler
I D
ID
Zhang
X R
XR
Zhang
J X
JX
Chen
Y F
YF
D000782:Aneuploidy; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D003703:Demecolcine / Q000494:pharmacology*; D005854:Germ Cells / Q000166:cytology*; D008297:Male; D008540:Meiosis / Q000187:drug effects*; D051379:Mice
D000972:Antineoplastic Agents, Phytogenic; D003703:Demecolcine