[Detection of Epstein-Barr virus in lymphoproliferative diseases by in situ hybridization].
OBJECTIVE
To study the relationship between Epstein-Barr virus and various lymphoproliferative diseases in China.


METHODS
Tissues from 214 patients with lymphoproliferative diseases were examined for the presence of Epstein-Barr virus (EBV) using in situ hybridization with EBV's LMP gene as probe.


RESULTS
The positive rate of EBV in Hodgkin's disease(HD), Non-Hodgkin's Lymphoma(NHL), benign lymphoid proliferative disease(BLP) was 30.0%(15/50), 14.0%(18/129), 2.9%(1/35), respectively. Among NHL, EBV was detected in 28.1%(9/32) of high grade lymphoma (HNHL), 10.5%(9/84) of medium grade lymphoma(MNHL) and in none(0/9) of low grade lymphoma(LNHL). Significantly higher rates than that of BLP were observed in HD and HNHL. No significant differences in rates among patients with MNHL, LNHL and BLP.


CONCLUSIONS
EBV may be involved in the pathogenesis of HD and HNHL, but not in MNHL and LNHL.
Xia
C
C
Department of Pathology, Beijing Chaoyang Hospital.
Liu
F
F
Sun
Y
Y
D000328:Adult; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006689:Hodgkin Disease / Q000821:virology*; D006801:Humans; D017403:In Situ Hybridization; D008228:Lymphoma, Non-Hodgkin / Q000821:virology*; D008232:Lymphoproliferative Disorders / Q000821:virology; D008875:Middle Aged; D014763:Viral Matrix Proteins / Q000032:analysis
C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins