title: Bestrophin, the product of the Best vitelliform macular dystrophy gene (VMD2), localizes to the basolateral plasma membrane of the retinal pigment epithelium.
abstract: Best vitelliform macular dystrophy is a dominantly inherited, early onset, macular degenerative disease that exhibits some histopathologic similarities to age-related macular degeneration. Although the vitelliform lesion is common in the fundus of individuals with Best disease, diagnosis is based on a reduced ratio of the light peak to dark trough in the electrooculogram. Recently, the VMD2 gene on chromosome 11q13, encoding the protein bestrophin, was identified. The function of bestrophin is unknown. To facilitate studies of bestrophin, we produced both rabbit polyclonal and mouse monoclonal antibodies that proved useful for Western blotting, immunoprecipitation, and immunocytochemistry. To characterize bestrophin, we initially probed the retinal pigment epithelium (RPE)-derived cell lines ARPE-19, D407, and RPE-J. All of the cell lines expressed bestrophin mRNA by reverse transcription-PCR, but not on Western blots. Bestrophin in human RPE partitioned in the detergent phase during Triton X-114 extraction and could be modified by biotin in intact cells, indicative of a plasma membrane localization. Immunocytochemical staining of macaque and porcine eyes indicated that bestrophin is localized at the basolateral plasma membrane of RPE cells. When expressed in RPE-J cells by adenovirus-mediated gene transfer, bestrophin again was determined by confocal microscopy and cell surface biotinylation to be a basolateral plasma membrane protein. The basolateral plasma membrane localization of bestrophin suggests the possibility that bestrophin plays a role in generating the altered electrooculogram of individuals with Best disease.
authors.affiliation: Department of Ophthalmic Research, Cole Eye Institute, and Department of Cell Biology, Lerner Research Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland OH 44195, USA. marmora@ccf.org
authors.forename: A D
authors.initials: AD
authors.lastname: Marmorstein
authors.forename: L Y
authors.initials: LY
authors.lastname: Marmorstein
authors.forename: M
authors.initials: M
authors.lastname: Rayborn
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.forename: J G
authors.initials: JG
authors.lastname: Hollyfield
authors.forename: K
authors.initials: K
authors.lastname: Petrukhin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D000075002:Bestrophins; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry; D018118:Chloride Channels; D005136:Eye Proteins / Q000032:analysis* / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D007473:Ion Channels; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010857:Pigment Epithelium of Eye / Q000737:chemistry* / Q000166:cytology; D011817:Rabbits; D012162:Retinal Degeneration / Q000235:genetics / Q000378:metabolism*
chemical_list: C113133:BEST1 protein, human; C491856:Best1 protein, mouse; D000075002:Bestrophins; D018118:Chloride Channels; D005136:Eye Proteins; D007473:Ion Channels