title: Effects of exercise on mitogen- and stress-activated kinase signal transduction in human skeletal muscle.
abstract: Exercise/contraction is a powerful stimulator of mitogen-activated protein (MAP) kinase cascades in skeletal muscle. Little is known regarding the physiological activation of enzymes downstream of MAP kinase. We investigated whether acute exercise results in activation of mitogen- and stress-activated kinases (MSK) 1 and 2, p90 ribosomal S6 kinase (p90rsk), and MAP kinase-activated protein kinase 2 (MAPKAPK2). Muscle biopsies were obtained from healthy volunteers before, during, and after 60 min one-leg cycle ergometry, from exercising and resting legs. MSK1 and MSK2 activities were increased 400-500% and 200-300%, respectively, in exercised muscle (P < 0.05 vs. rest). A dramatic increase in activity of p90rsk (MAPKAPK1) (>2,500%), and to a lesser extent MAPKAP2 (300%), was noted with exercise (P < 0.05 vs. rest). MSK1, MSK2, p90rsk, and MAPKAP2 activities were sustained throughout exercise. Exercise-induced activation of these enzymes was limited to working muscle, indicating that local rather than systemic factors activate these signaling cascades. Thus physical exercise leads to activation of multiple enzymes downstream of MAP kinase.
authors.affiliation: Department of Clinical Physiology, Karolinska Hospital, Stockholm University College of Physical Education and Sports, Stockholm, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Krook
authors.forename: U
authors.initials: U
authors.lastname: Widegren
authors.forename: X J
authors.initials: XJ
authors.lastname: Jiang
authors.forename: J
authors.initials: J
authors.lastname: Henriksson
authors.forename: H
authors.initials: H
authors.lastname: Wallberg-Henriksson
authors.forename: D
authors.initials: D
authors.lastname: Alessi
authors.forename: J R
authors.initials: JR
authors.lastname: Zierath
mesh_terms: D000328:Adult; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D004789:Enzyme Activation; D015444:Exercise / Q000502:physiology*; D005260:Female; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D020935:MAP Kinase Signaling System; D008297:Male; D009119:Muscle Contraction; D018482:Muscle, Skeletal / Q000201:enzymology*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D019893:Ribosomal Protein S6 Kinases / Q000378:metabolism; D038744:Ribosomal Protein S6 Kinases, 90-kDa; D000099190:MAP-Kinase-Activated Kinase 2
chemical_list: D017871:Calcium-Calmodulin-Dependent Protein Kinases; D047908:Intracellular Signaling Peptides and Proteins; D017346:Protein Serine-Threonine Kinases; D019893:Ribosomal Protein S6 Kinases; D038744:Ribosomal Protein S6 Kinases, 90-kDa; D000099190:MAP-Kinase-Activated Kinase 2; C115634:RPS6KA4 protein, human; C116919:mitogen and stress-activated protein kinase 1