The Rab6-binding kinesin, Rab6-KIFL, is required for cytokinesis.
The Rab6-binding kinesin, Rab6-KIFL, was identified in a two-hybrid screen for proteins that interact with Rab6, a small GTPase involved in membrane traffic through the Golgi apparatus. We find that Rab6-KIFL accumulates in mitotic cells where it localizes to the midzone of the spindle during anaphase, and to the cleavage furrow and midbody during telophase. Overexpression of Rab6-KIFL causes a cell division defect resulting in cell death. Microinjection of antibodies to Rab6-KIFL results in the cells becoming binucleate after one cell cycle, and time-lapse microscopy reveals that this is due to a defect in cleavage furrow formation and thus cytokinesis. These data show that endogenous Rab6-KIFL functions in cell division during cleavage furrow formation and cytokinesis, in addition to its previously described role in membrane traffic.
Hill
E
E
Beatson Institute for Cancer Research, and University of Glasgow Institute of Biological and Life Sciences, CRC-Beatson Laboratories, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, UK.
Clarke
M
M
Barr
F A
FA
D001483:Base Sequence; D002455:Cell Division / Q000502:physiology*; D017931:DNA Primers / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D016547:Kinesins / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D008856:Microscopy, Fluorescence; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D014162:Transfection; D020798:Two-Hybrid System Techniques; D020691:rab GTP-Binding Proteins / Q000502:physiology*
D017931:DNA Primers; C094135:Rab6 protein; D011994:Recombinant Proteins; D016547:Kinesins; D020691:rab GTP-Binding Proteins