Rab6c, a new member of the rab gene family, is involved in drug resistance in MCF7/AdrR cells.
A new Rab6 homolog cDNA, Rab6c, was discovered by a hypermethylated DNA fragment probe that was isolated from a human multidrug resistant (MDR) breast cancer cell line, MCF7/AdrR, by the methylation sensitive-representational difference analysis (MS-RDA) technique. Rab6c was found to be under-expressed in MCF7/AdrR and MES-SA/Dx5 (a human MDR uterine sarcoma cell line) compared with their non-MDR parental cell lines. MCF7/AdrR cells expressing the exogenous Rab6c exhibited less resistance to several anti-cancer drugs, such as doxorubicin (DOX), taxol, vinblastine, and vincristine, than the control cells containing the empty vector. Flow cytometry experiments confirmed that the transfectants' diminished resistance to DOX was caused by increased drug accumulation induced by the exogenous Rab6c. These results indicate that Rab6c is involved in drug resistance in MCF7/AdrR cells.
Shan
J
J
Molecular Oncology, North Shore-Long Island Jewish Health System, New York University School of Medicine, New York, NY 11030, USA.
Mason
J M
JM
Yuan
L
L
Barcia
M
M
Porti
D
D
Calabro
A
A
Budman
D
D
Vinciguerra
V
V
Xu
H
H
D000595:Amino Acid Sequence; D000970:Antineoplastic Agents / Q000494:pharmacology; D001483:Base Sequence; D002455:Cell Division / Q000187:drug effects / Q000235:genetics; D019175:DNA Methylation; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004274:DNA, Recombinant; D004317:Doxorubicin / Q000494:pharmacology; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D017422:Sequence Analysis, DNA; D014162:Transfection; D014407:Tumor Cells, Cultured; D020691:rab GTP-Binding Proteins / Q000235:genetics*
D000970:Antineoplastic Agents; D018076:DNA, Complementary; D004274:DNA, Recombinant; D004317:Doxorubicin; C417211:RAB6C protein, human; D020691:rab GTP-Binding Proteins