A comparison of DNA vaccines expressing the 45W, 18k and 16k host-protective antigens of Taenia ovis in mice and sheep.
The immunogenicity of DNA vaccines encoding three different Taenia ovis host-protective antigens was compared in mice and sheep. DNA vaccines encoding the 45W, 18k and 16k antigens of T. ovis were constructed. The ability of DNA vaccines encoding the 45W and 18k genes to express antigen was confirmed by Western blotting of transfected Cos-7 cells. BALB/c mice were vaccinated intramuscularly with 45W, 18k or 16k DNA vaccines and the humoral immune response analysed by ELISA. DNA vaccines expressing 45W, 18k or 16k antigen were immunogenic in mice and generated significant titres of antigen-specific antibody. Intramuscular vaccination of outbred sheep with the T. ovis DNA vaccines generated significantly lower titres of 45W-specific antibody and failed to generate 18k or 16k-specific antibody. The findings of this study show that each of the three T. ovis host-protective antigens are amenable to delivery via DNA vaccines, and that the parameters governing the efficacy of DNA vaccines in sheep require further investigation.
Drew
D R
DR
Department of Microbiology and Immunology, The University of Melbourne, Parkville, Vic., Australia.d.drew@pgrad.unimelb.edu.au
Lightowlers
M W
MW
Strugnell
R A
RA
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D015153:Blotting, Western / Q000662:veterinary; D019556:COS Cells; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology* / Q000469:parasitology; D013621:Taenia / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D013622:Taeniasis / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D014611:Vaccination / Q000662:veterinary*; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology* / Q000592:standards
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D019444:Vaccines, DNA