title: Cloning and functional expression of a human orthologue of rat vanilloid receptor-1.
abstract: Capsaicin, resiniferatoxin, protons or heat have been shown to activate an ion channel, termed the rat vanilloid receptor-1 (rVR1), originally isolated by expression cloning for a capsaicin sensitive phenotype. Here we describe the cloning of a human vanilloid receptor-1 (hVR1) cDNA containing a 2517 bp open reading frame that encodes a protein with 92% homology to the rat vanilloid receptor-1. Oocytes or mammalian cells expressing this cDNA respond to capsaicin, pH and temperature by generating inward membrane currents. Mammalian cells transfected with human VR1 respond to capsaicin with an increase in intracellular calcium. The human VR1 has a chromosomal location of 17p13 and is expressed in human dorsal root ganglia and also at low levels throughout a wide range of CNS and peripheral tissues. Together the sequence homology, similar expression profile and functional properties confirm that the cloned cDNA represents the human orthologue of rat VR1.
authors.affiliation: Department of Biotechnology and Genetics, SmithKline Beecham Pharmaceuticals, New Frontiers Science Park North, Third Avenue, Harlow CM19 5AW, UK Department of Neuroscience Research, SmithKline Beecham Pharmaceuticals, New Frontiers Science Park North, Third Avenue, Harlow CM19 5AW, UK Department of Bioinformatics, SmithKline Beecham Pharmaceuticals, New Frontiers Science Park North, Third Avenue, Harlow CM19 5AW, UK.
authors.forename: Philip
authors.initials: P
authors.lastname: Hayes
authors.forename: Helen J
authors.initials: HJ
authors.lastname: Meadows
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Gunthorpe
authors.forename: Mark H
authors.initials: MH
authors.lastname: Harries
authors.forename: Malcolm D
authors.initials: MD
authors.lastname: Duckworth
authors.forename: William
authors.initials: W
authors.lastname: Cairns
authors.forename: David C
authors.initials: DC
authors.lastname: Harrison
authors.forename: Catherine E
authors.initials: CE
authors.lastname: Clarke
authors.forename: Kathryn
authors.initials: K
authors.lastname: Ellington
authors.forename: Rab K
authors.initials: RK
authors.lastname: Prinjha
authors.forename: Amanda J L
authors.initials: AJL
authors.lastname: Barton
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Medhurst
authors.forename: Graham D
authors.initials: GD
authors.lastname: Smith
authors.forename: Simon
authors.initials: S
authors.lastname: Topp
authors.forename: Paul
authors.initials: P
authors.lastname: Murdock
authors.forename: Gareth J
authors.initials: GJ
authors.lastname: Sanger
authors.forename: John
authors.initials: J
authors.lastname: Terrett
authors.forename: Owen
authors.initials: O
authors.lastname: Jenkins
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Benham
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Randall
authors.forename: Isro S
authors.initials: IS
authors.lastname: Gloger
authors.forename: John B
authors.initials: JB
authors.lastname: Davis
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002875:Chromosomes / Q000235:genetics; D003001:Cloning, Molecular; D004247:DNA / Q000096:biosynthesis / Q000235:genetics; D005838:Genotype; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009619:Nociceptors / Q000187:drug effects / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism; D011110:Polymorphism, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D011955:Receptors, Drug / Q000096:biosynthesis* / Q000187:drug effects / Q000235:genetics*; D050916:TRPV Cation Channels; D013696:Temperature; D014981:Xenopus
chemical_list: D012333:RNA, Messenger; D011955:Receptors, Drug; D050916:TRPV Cation Channels; C494484:TRPV1 receptor; D004247:DNA