title: The role of actin cytoskeleton in the generation of surface oscillations of red blood cell ghosts.
abstract: We have studied the effects of three compounds on surface oscillations of human red blood cell ghosts: the P-ATPase inhibitor, suramin; the fluorescent dye of a similar structure, 1,8-anilinonaphthalene sulfonate (ANS); and subfragment 1 of skeletal muscle myosin (S1). It has been found that suramin (10 microM), ANS (100 microM) and S1 (2 mg/ml) suppress the surface oscillations reversibly. The shape of the ghosts remains unchanged. We have also found that suramin and ANS inhibit the ghosts' non-transport (presumably, F-actin-associated) ATPase. The results of the present study suggest the important role of actin ATPase in the generation of cell surface oscillations. The effect of S1, the protein which increases the torsional, but not the bending, rigidity of F-actin upon binding to filaments, favours the possibility that just the torsional dynamics of actin protofilaments leads to the observed oscillations of the ghosts' surface.
authors.affiliation: Institute of Cytology, Russian Academy of Sciences, St. Petersburg.
authors.forename: A Y
authors.initials: AY
authors.lastname: Krol
authors.forename: M G
authors.initials: MG
authors.lastname: Grinfeldt
authors.forename: A A
authors.initials: AA
authors.lastname: Vereninov
authors.forename: V V
authors.initials: VV
authors.lastname: Malev
mesh_terms: D000199:Actins / Q000378:metabolism*; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000817:Anilino Naphthalenesulfonates / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D003599:Cytoskeleton / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000502:physiology*; D005456:Fluorescent Dyes / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D009218:Myosins / Q000378:metabolism / Q000494:pharmacology; D010590:Phalloidine / Q000378:metabolism / Q000494:pharmacology; D011817:Rabbits; D013498:Suramin / Q000378:metabolism / Q000494:pharmacology
chemical_list: D000199:Actins; D000817:Anilino Naphthalenesulfonates; D005456:Fluorescent Dyes; D010590:Phalloidine; D013498:Suramin; C027132:1-anilino-8-naphthalenesulfonate; D000251:Adenosine Triphosphatases; D009218:Myosins