Electrochemical studies of the effect of lanthanide ions on the activity of glutamate dehydrogenase.
Using the electrochemical method based on the "diffusion" layer concept of the convective system, the effect of lanthanide ions La3+ and Eu3+ on the activity of glutamate dehydrogenase (GLDH) has been determined. In suitable concentrations, the lanthanide ions La3+ and Eu3+ can activate the GLDH in the reductive amination of alpha-Ketoglutarate (alpha-KG). The activation mechanism would be that the lanthanide ions could interrupt the binding of NAD+ to GLDH by combining preferentially to NAD+. This mechanism was proposed by the voltammetric studies.
Zhuang
Q K
QK
Department of Chemistry, Beijing, People's Republic of China. qkzhuang@chemms.chem.pku.edu.cn
Dai
H C
HC
Gao
X X
XX
Xin
W K
WK
D004563:Electrochemistry; D004789:Enzyme Activation; D005969:Glutamate Dehydrogenase / Q000378:metabolism*; D008674:Metals, Rare Earth / Q000494:pharmacology*; D009243:NAD / Q000378:metabolism; D011485:Protein Binding
D008674:Metals, Rare Earth; D009243:NAD; D005969:Glutamate Dehydrogenase