title: Optimization of conditions for safflower cell culture and accumulation of cellicolous product tocopherols.
abstract: The culture of calli, which were induced from cotyledon of Carthamus tinctorius L, was able to synthesize tocopherols. Results determined by NP-HPLC showed that the main component was alpha-tocopherol. When 0.1% casein was added into MS medium, and 2,4-D concentration was adjucted to 0.30 mg/L and 6-BA to 1.80 mg/L, alpha-, beta- and gamma-forms of tocopherol reached 4.1706, 0.3120 and 0.1650 microg/g fresh calli, respectively. The effective tocopherol content was 4.4091 microg/g fresh calli, and was 57 times higher than that in the control.
authors.affiliation: Bio-environment Engineering Institute of Zhejiang University, Huajiachi, Hangzhou, China.
authors.forename: P
authors.initials: P
authors.lastname: Wang
authors.forename: S
authors.initials: S
authors.lastname: Ou
authors.forename: C
authors.initials: C
authors.lastname: Peilin
mesh_terms: D019659:Asteraceae / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods*; D014810:Vitamin E / Q000096:biosynthesis*
chemical_list: D014810:Vitamin E