Structure of the O-specific polysaccharide of the bacterium Proteus vulgaris O23.
An acidic O-specific polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of the bacterium Proteus vulgaris O23 (strain PrK 44/57) and found to contain 2-acetamido-2-deoxy-D-galactose, 2-acetamido-2-deoxy-D-glucose, and D-galacturonic acid. Based on 1H- and 13C-NMR spectroscopic studies, including two-dimensional correlation spectroscopy (COSY), total correlation spectroscopy (TOCSY), nuclear Overhauser effect spectroscopy (NOESY), and 1H,13C heteronuclear multiple-quantum coherence (HMQC) experiments, the following structure of the branched tetrasaccharide repeating unit of the polysaccharide was established: [figure], where the degree of O-acetylation of the terminal GalA residue at position 4 is about 80%. A structural similarity of the O-specific polysaccharides of P. vulgaris O23 and P. mirabilis O23 is discussed.
Perepelov
A V
AV
Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, 117913, Russia.
Shashkov
A S
AS
Babichka
D
D
Senchenkova
S N
SN
Bartodziejska
B
B
Rozalski
A
A
Knirel
Y A
YA
D000107:Acetylation; D002240:Carbohydrate Sequence; D002849:Chromatography, Gas; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry* / Q000302:isolation & purification; D011134:Polysaccharides / Q000737:chemistry*; D011514:Proteus vulgaris / Q000737:chemistry* / Q000276:immunology
D019081:O Antigens; D011134:Polysaccharides