Caseinomacropeptide specifically stimulates exocrine pancreatic secretion in the anesthetized rat.
The effect of caseinomacropeptide (CMP) (the [106-169] fragment of kappa-casein produced during digestion of milk protein), was studied in anesthetized rats using bile diversion for a pure pancreatic juice collection system. Intraduodenal administration of CMP induced a dose-related specific stimulation of pancreatic secretion which was nearly abolished by devazepide, atropine, hexamethonium, vagotomy or perivagal capsaicin pretreatment. Moreover, CMP did not inhibit in vitro trypsin activity. These results demonstrate that CMP is more likely to stimulate pancreatic secretion specifically through cholecystokinin release and activation of a vago-vagal cholinergic reflex loop than by inhibition of luminal trypsin, in anesthetized rats.
Pedersen
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UMR PNCA, Unité de Physiologie de la Nutrition et du Comportement Alimentaire, INA P-G, 16 rue Claude Bernard, F-75231 Cedex 05, Paris, France.
Nagain-Domaine
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C
Mahé
S
S
Chariot
J
J
Rozé
C
C
Tomé
D
D
D000758:Anesthesia; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D002211:Capsaicin / Q000494:pharmacology; D002364:Caseins / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D020109:Devazepide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D005983:Glutens / Q000494:pharmacology; D018738:Hexamethonium / Q000494:pharmacology; D008297:Male; D008894:Milk Proteins / Q000494:pharmacology; D008954:Models, Biological; D010179:Pancreas / Q000187:drug effects* / Q000294:innervation / Q000378:metabolism*; D010189:Pancreatic Juice / Q000187:drug effects / Q000378:metabolism; D010446:Peptide Fragments / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D011949:Receptors, Cholecystokinin / Q000037:antagonists & inhibitors; D012844:Sincalide / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D014357:Trypsin / Q000378:metabolism; D014361:Trypsin Inhibitors / Q000494:pharmacology; D014628:Vagotomy; D000067816:Whey Proteins
D002364:Caseins; D008894:Milk Proteins; D010446:Peptide Fragments; D011949:Receptors, Cholecystokinin; D014361:Trypsin Inhibitors; D000067816:Whey Proteins; C065989:caseinomacropeptide; D018738:Hexamethonium; D012965:Sodium Chloride; D001285:Atropine; D005983:Glutens; D014357:Trypsin; D020109:Devazepide; D012844:Sincalide; D002211:Capsaicin