The immunocytochemical versus cytosol measurement of the oestrogen receptor in invasive breast cancer tissue.
To compare two methods of measurement of oestrogen receptor (ER)-expression in invasive breast cancer tissue. Sections from 299 breast cancer cases were stained for the ER by immunocytochemical assay (ICA), using mouse monoclonal antibody (MAb) NCL-ER-6SF11, and by the dextran-coated charcoal assay (DCC). Concordant results were observed in 230 of the 299 cases (77%), 69 patients had discordant results (kappa=0.537). We found a moderate concordance between ICA and DCC for ER measurement in breast cancer tissue. If we change the golden standard from DCC to ICA, 23% of patients would receive a different therapeutic approach.
Gordts
S L
SL
Department of Obstet. and Gyn., Algemene Kliniek St-Jan, Broekstraat 104, 1000, Brussel, Belgium.
Neven
P
P
Van Hooff
I
I
Tonglet
R
R
Laurent
C
C
Marechal
S
S
De Muylder
X
X
Van Belle
Y
Y
Vanderick
G
G
D014408:Biomarkers, Tumor / Q000032:analysis*; D001943:Breast Neoplasms / Q000737:chemistry* / Q000473:pathology; D003600:Cytosol / Q000737:chemistry*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D009361:Neoplasm Invasiveness; D011960:Receptors, Estrogen / Q000032:analysis*
D014408:Biomarkers, Tumor; D011960:Receptors, Estrogen