The dnaJ (hsp40) locus in Rhizobium leguminosarum bv. phaseoli is required for the establishment of an effective symbiosis with Phaseolus vulgaris.
P121R25 is a Tn5-induced mutant of the effective Rhizobium leguminosarum bv. phaseoli strain P121R that is unable to use glutamate as the sole carbon and nitrogen source and is defective in symbiotic nitrogen fixation. Enzymatic analysis showed that three enzymes implicated in glutamate metabolism (glutamate dehydrogenase, 2-oxoglutarate dehydrogenase, and glutamate synthase) were affected by this mutation. Sequencing of the chromosomal locus bordering the Tn5 in P121R25 indicated the presence of the dnaK and dnaJ genes in an arrangement similar to that described in R. leguminosarum bv. viciae (GenBank accession number Y14649). The mutation was located in the dnaJ (hsp40) gene.
Labidi
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Département des Sols et de Génie Agroalimentaire, Faculté des Sciences de l'Agriculture et de l'Alimentation, Université Laval, Québec, Qc, Canada.
Laberge
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S
Vézina
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LP
Antoun
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D002874:Chromosome Mapping; D007887:Fabaceae / Q000382:microbiology*; D005798:Genes, Bacterial; D018698:Glutamic Acid / Q000378:metabolism; D050956:HSP40 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009586:Nitrogen Fixation; D010946:Plants, Medicinal; D016961:Rhizobium leguminosarum / Q000502:physiology*; D013559:Symbiosis
D050956:HSP40 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D018698:Glutamic Acid