title: Strain-dependent antibody response induced by DNA immunization.
abstract: The antibody (Ab) response induced by DNA-based immunization was compared in various strains of inbred, H-2 congenic and outbred mice with different haplotypes of mouse major histocompatibility complex (H-2). Two different plasmid expression vectors encoding Aequorea victoria green fluorescent protein (GFP) or Escherichia coli, beta-galactosidase (beta-gal) were introduced into quadriceps muscle, and Ab production was examined using both enzyme-linked immunosorbent assay and immunoblot analysis. The beta-gal plasmid DNA immunization induced strong Ab production in all inbred, H-2 congenic and outbred strains at the early stages of immunization. By comparison with beta-gal peptide immunization, the degree of Ab response was H-2 haplotype-dependent. On the other hand, Ab production by GFP plasmid DNA immunization was observed in outbred strains, but not in some of the inbred and H-2 congenic strains. Also, outbred strains showed a high Ab response compared with other inbred and H-2 congenic strains by GFP peptide immunization. Reverse transcription-polymerase chain reaction analysis demonstrated the presence of GFP or beta-gal transcripts at the DNA inoculation site in all the strains studied, even in inbred and H-2 congenic strains which showed no Ab production by GFP plasmid DNA immunization. These results indicate that the difference in Ab response induced by DNA immunization as well as by peptide immunization depends upon the H-2 haplotypes of host strains.
authors.affiliation: Kato Cytoprotein Network Project, ERATO, Japan Science and Technology (JST), c/o Sagami Chemical Research Center, 4-4-1 Nishi-ohnuma, 229-0012, Sagamihara, Japan. koichi@med.kitasato-u.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Ito
authors.forename: Y
authors.initials: Y
authors.lastname: Takeuchi
authors.forename: K
authors.initials: K
authors.lastname: Ito
authors.forename: S
authors.initials: S
authors.lastname: Kato
mesh_terms: D000818:Animals; D020296:Animals, Congenic / Q000276:immunology; D021461:Animals, Outbred Strains / Q000276:immunology; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000276:immunology; D000917:Antibody Formation; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology; D005260:Female; D049452:Green Fluorescent Proteins; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology*; D006239:Haplotypes / Q000235:genetics; D007273:Injections, Intramuscular; D008164:Luminescent Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D051379:Mice / Q000235:genetics / Q000276:immunology*; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D008815:Mice, Inbred Strains / Q000276:immunology; D010957:Plasmids / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D037401:Scyphozoa / Q000235:genetics / Q000276:immunology; D013045:Species Specificity; D019444:Vaccines, DNA / Q000276:immunology*; D001616:beta-Galactosidase / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D006183:H-2 Antigens; D008164:Luminescent Proteins; D012333:RNA, Messenger; D019444:Vaccines, DNA; D049452:Green Fluorescent Proteins; D001616:beta-Galactosidase