Differential localization of septins in the mouse brain.
We have carried out a comparative immunohistochemical study on four members of the septin family, CDCrel-1, Septin6, CDC10, and H5, which are abundantly expressed in the adult mouse brain. We found that each septin showed overlapping but distinct distribution at the levels of light and electron microscopy. CDCrel-1 was abundant in inhibitory presynaptic terminals and associated with GABAergic vesicles in the thalamus, globus pallidus, and cerebellar nuclei. Septin6 was associated with synaptic vesicles in various brain regions, including glomeruli of the olfactory bulb. CDC10 was diffusely expressed in the brain and was localized beneath presynaptic membrane and astroglial processes. H5 was localized in the astroglial processes in some specific brain regions. The differential expression and subcellular localization of these septins indicates that a given neuron or glial cell expresses a specific set of septin monomers and that the resulting septin complexes with distinct compositions may play distinct roles in the brain.
Kinoshita
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Department of Neurology, Kyoto University Graduate School of Medicine, Kyoto 606-8501, Japan.
Noda
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Kinoshita
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D000818:Animals; D001253:Astrocytes / Q000737:chemistry*; D001923:Brain Chemistry / Q000502:physiology; D018797:Cell Cycle Proteins / Q000032:analysis; D020558:GTP Phosphohydrolases; D008297:Male; D008565:Membrane Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000032:analysis*; D010455:Peptides; D017729:Presynaptic Terminals / Q000737:chemistry*; D011506:Proteins / Q000032:analysis; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D058112:Septins; D013572:Synaptic Vesicles / Q000737:chemistry*; D014157:Transcription Factors
C090459:CDC1 protein, S cerevisiae; D018797:Cell Cycle Proteins; C082750:H5 protein, synthetic; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D010455:Peptides; D011506:Proteins; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors; C061178:cdc10 protein, S pombe; C512892:CDC10 protein, S cerevisiae; D020558:GTP Phosphohydrolases; C512895:Sept7 protein, mouse; D058112:Septins