title: Expression of GABA(A) receptor alpha5 subunit-like immunoreactivity in human hippocampus.
abstract: To investigate the distribution of GABA(A) receptor alpha5 subunit expression in brain, polyclonal antisera were raised, characterised and applied to human and rat brain sections. The resultant antibodies detected a major band of 53 kDa in sodium dodecyl sulphate-polyacrylamide gel electrophoresis immunoblots. Abundant immunostaining was demonstrated in the hippocampal formation in multiple cell types, although predominantly in pyramidal neurons. These data are supportive of GABA-ergic involvement in cognition, and suggest that this influence may be mediated through receptors containing the alpha5 subunit.
authors.affiliation: Merck, Sharp and Dohme Research Laboratories, Neuroscience Research Centre, Terling Park, Harlow, CM20 2QR, Essex, UK.
authors.forename: A
authors.initials: A
authors.lastname: Wainwright
authors.forename: D J
authors.initials: DJ
authors.lastname: Sirinathsinghji
authors.forename: K R
authors.initials: KR
authors.lastname: Oliver
mesh_terms: D000818:Animals; D000906:Antibodies; D000918:Antibody Specificity; D006624:Hippocampus / Q000737:chemistry*; D006801:Humans; D007150:Immunohistochemistry; D051381:Rats; D011963:Receptors, GABA-A / Q000032:analysis* / Q000276:immunology
chemical_list: D000906:Antibodies; D011963:Receptors, GABA-A