title: Enteral inulin does not affect epithelial gene expression and cell turnover within the ileoanal pouch.
abstract: OBJECTIVE
  This study evaluates the effects of enteral inulin on ileoanal pouch functioning by studying epithelial gene expression, cell turnover, and mucosal morphology.
  
  
  METHODS
  Twenty patients with an ileoanal pouch received 24 g of inulin daily for three weeks, then a four-week wash-out period, and a placebo for three weeks. In this randomized, double-blind, crossover study, biopsy specimens of pouch mucosa were taken after each test period. Mucosal morphology, inflammation, epithelial proliferation, and cell death were assessed histologically. Expressions of proapoptotic and antiapoptotic regulators, intestinal fatty acid-binding protein, and mucin were quantified by Western blotting or enzyme-linked immunosorbent assay. The number of intestinal fatty acid-binding protein expressing cells was histologically assessed and a high iron diamine/Alcian blue staining was performed to discriminate between sulfated and nonsulfated acidic mucins.
  
  
  RESULTS
  Inulin supplementation neither altered mucosal morphology nor influenced inflammation, epithelial cell proliferation, or cell death. The ratio between the proapoptotic and antiapoptotic regulators did not change after inulin supplementation. The number of intestinal fatty acid-binding protein-producing enterocytes and the intestinal fatty acid-binding protein expression level increased after inulin treatment, but did not reach statistical significance. The intestinal fatty acidbinding protein expression level correlated with the Pouchitis Disease Activity Index, which was at the brink of significance (P = 0.06). Mucin expression and the ratio between sulfated and nonsulfated acidic mucins were not altered by inulin supplementation.
  
  
  CONCLUSIONS
  In this prospective study, inulin supplementation did not significantly alter pouch mucosal functioning because neither epithelial homeostasis nor epithelial gene expression was significantly altered by enteral inulin.
authors.affiliation: Department Pediatrics, Erasmus University Rotterdam, Sophia Children's Hospital, The Netherlands.
authors.forename: H P
authors.initials: HP
authors.lastname: Meijer
authors.forename: C F
authors.initials: CF
authors.lastname: Welters
authors.forename: E
authors.initials: E
authors.lastname: Heineman
authors.forename: G S
authors.initials: GS
authors.lastname: Salomons
authors.forename: H A
authors.initials: HA
authors.lastname: Büller
authors.forename: J
authors.initials: J
authors.lastname: Dekker
authors.forename: A W
authors.initials: AW
authors.lastname: Einerhand
mesh_terms: D000328:Adult; D017209:Apoptosis; D002455:Cell Division; D018592:Cross-Over Studies; D004311:Double-Blind Method; D004847:Epithelial Cells / Q000187:drug effects* / Q000502:physiology; D005232:Fatty Acids, Volatile / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D006706:Homeostasis; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D007444:Inulin / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D016737:Proctocolectomy, Restorative; D011446:Prospective Studies
chemical_list: D005232:Fatty Acids, Volatile; D007444:Inulin