title: Mutational and hyperexpression-induced disruption of bipolar budding in yeast.
abstract: Analysis of bud-site selection in the yeast Saccharomyces cerevisiae has helped to identify many genes that are generally important for eukaryotic cell polarization. Colony morphology screens were used to identify factors relevant to the process of bipolar budding in yeast. Mutants defective in bipolar budding were identified by virtue of their inability to grow as pseudohyphae in a haploid bud3 background. A mutant allele of the MYO2 gene, encoding a class-V unconventional myosin was identified that perturbs bipolar budding without affecting axial budding and without grossly affecting the role of Myo2p in secretion and maintenance of the actin cytoskeleton. Several genes were also identified whose products, when overexpressed, are capable of disrupting bipolar budding. Among these are the actin-monomer-binding protein profilin and the Aip3p/Bud6p-interacting protein Atc1p. The results strongly support involvement of the actin cytoskeleton in the establishment of bipolar budding and in the maintenance of pseudohyphal growth.
authors.affiliation: Section of Molecular Cell and Developmental Biology, The University of Texas, Austin, TX 78712, USA2.|Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138, USA1.
authors.forename: Toby
authors.initials: T
authors.lastname: Freedman
authors.affiliation: Section of Molecular Cell and Developmental Biology, The University of Texas, Austin, TX 78712, USA2.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Porter
authors.affiliation: Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 E. Adams St, Syracuse, NY 13210, USA3.|Section of Molecular Cell and Developmental Biology, The University of Texas, Austin, TX 78712, USA2.
authors.forename: Brian
authors.initials: B
authors.lastname: Haarer
mesh_terms: D000483:Alleles; D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology; D002455:Cell Division / Q000235:genetics / Q000502:physiology; D016764:Cell Polarity / Q000235:genetics / Q000502:physiology; D003285:Contractile Proteins; D003599:Cytoskeleton / Q000235:genetics / Q000502:physiology; D005656:Fungal Proteins / Q000235:genetics / Q000502:physiology; D015870:Gene Expression; D005800:Genes, Fungal; D008840:Microfilament Proteins / Q000235:genetics / Q000502:physiology; D009154:Mutation; D018995:Myosin Heavy Chains; D024462:Myosin Type II; D024701:Myosin Type V; D009218:Myosins / Q000235:genetics / Q000502:physiology; D051304:Profilins; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development* / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins
chemical_list: C107727:BUD6 protein, S cerevisiae; D002352:Carrier Proteins; D003285:Contractile Proteins; D005656:Fungal Proteins; C077512:MYO2 protein, S cerevisiae; C434211:MYO2 protein, S pombe; D008840:Microfilament Proteins; D051304:Profilins; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D024462:Myosin Type II; D024701:Myosin Type V; D018995:Myosin Heavy Chains; D009218:Myosins