Detection of Ehrlichia platys DNA in brown dog ticks (Rhipicephalus sanguineus) in Okinawa Island, Japan.
Thirty-two Rhipicephalus sanguineus females collected from eight free-roaming dogs in Okinawa Island, Japan, were examined for ehrlichial DNA by 16S rRNA-based PCR and subsequent sequencing. Partial sequences of Ehrlichia platys 16S rRNA (678 to 679 bp) were detected in three ticks (9.4%) from two dogs. This is the first report of detection of E. platys in Japan, and also the first report of detection in ticks.
Inokuma
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Laboratory of Veterinary Internal Medicine, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan.
Raoult
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Brouqui
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D000818:Animals; D004269:DNA, Bacterial / Q000032:analysis; D004285:Dogs; D004536:Ehrlichia / Q000235:genetics* / Q000302:isolation & purification*; D005260:Female; D020459:Genes, rRNA; D007564:Japan; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013984:Tick Infestations / Q000469:parasitology*; D013987:Ticks / Q000382:microbiology*
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S