title: Indocyanine green-assisted peeling of the retinal internal limiting membrane.
abstract: OBJECTIVE
  To determine whether indocyanine green (ICG) stains and facilitates peeling of the retinal internal limiting membrane (ILM). To investigate the different staining properties of the posterior cortical hyaloid, retinal ILM, and the retina after ILM removal.
  
  
  METHODS
  Autopsy eye study.
  
  
  METHODS
  Eleven human cadaveric eyes.
  
  
  METHODS
  Open sky vitrectomy including removal of the posterior cortical vitreous was performed. A 0.5% ICG solution was then injected into the posterior vitreous cavity over the macula. The dye was allowed to settle on the macula for 5 minutes and was then removed by mechanical aspiration. Peeling of the ILM was initiated with a bent needle and completed with intraocular forceps. Specimens were submitted for light and electron microscopy.
  
  
  METHODS
  Staining properties and ease of peeling of retinal ILM were evaluated. Retinal ILM removal was confirmed by histopathologic and electron microscopic examination.
  
  
  RESULTS
  ICG contact with the retinal surface resulted in bright green staining of the ILM. This stain greatly facilitated ILM peeling by improving direct visualization of the membrane. The underlying retina did not stain, thus providing a clear distinction between the stained ILM and the unstained retina. Continuous circular peeling of the ILM was easily completed with this technique. Light microscopic and ultrastructural studies confirmed removal of the ILM.
  
  
  CONCLUSIONS
  ICG solution distinctly stains the nearly invisible retinal ILM in human cadaveric eyes. ICG staining greatly facilitates ILM peeling by providing a stark contrast between the stained ILM and the unstained retina.
authors.affiliation: Cincinnati Eye Institute, Cincinnati, Ohio 45242, USA.
authors.forename: S E
authors.initials: SE
authors.lastname: Burk
authors.forename: A P
authors.initials: AP
authors.lastname: Da Mata
authors.forename: M E
authors.initials: ME
authors.lastname: Snyder
authors.forename: R H
authors.initials: RH
authors.lastname: Rosa
authors.forename: R E
authors.initials: RE
authors.lastname: Foster
mesh_terms: D001485:Basement Membrane / Q000601:surgery / Q000648:ultrastructure; D004396:Coloring Agents; D003941:Diagnostic Techniques, Ophthalmological; D019773:Epiretinal Membrane / Q000175:diagnosis* / Q000601:surgery*; D006801:Humans; D007208:Indocyanine Green; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D004396:Coloring Agents; D007208:Indocyanine Green