Purification, crystallization and preliminary X-ray crystallographic analysis of human CCG1-interacting factor B.
A novel human factor CIB (CCG1-interacting factor B) has been isolated using the yeast two-hybrid system. The 22 kDa CIB protein has been expressed in Escherichia coli, purified to homogeneity and crystallized in a form suitable for crystallographic studies. The protein was crystallized in the orthogonal space group P2(1)2(1)2(1), with unit-cell parameters a = 43.60 (2), b = 44.45 (1), c = 110.70 (5) A and one molecule in the asymmetric unit. The crystal diffracted beyond 2.2 A resolution using synchrotron radiation.
Padmanabhan
B
B
Horikoshi Gene Selector Project, Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology Corporation (JST), 5-9-6 Tokodai, Tsukuba, Ibaraki 300-2635, Japan.
Kuzuhara
T
T
Mizuno
H
H
Horikoshi
M
M
D018797:Cell Cycle Proteins / Q000737:chemistry*; D003461:Crystallography; D018360:Crystallography, X-Ray; D006801:Humans; D016366:Open Reading Frames; D011487:Protein Conformation
D018797:Cell Cycle Proteins