title: The effect of Golgi depletion on exocytic transport.
abstract: Here we evaluate the idea that the Golgi is in dynamic equilibrium with the endoplasmic reticulum (ER). In cytoplasts that lack the Golgi apparatus, no regrowth of the Golgi is observed, nor is any transport from the ER to the cell surface detected. However, introduction of the smallest measurable amount of Golgi (equivalent to a few per cent per cell) yields significant exocytic transport. Our results indicate that the steady-state levels of Golgi in the ER are far smaller than the 30% that has been postulated, and that the Golgi may be an independent organelle and not simply an extension of the ER.
authors.affiliation: Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06520-8002, USA.
authors.forename: L
authors.initials: L
authors.lastname: Pelletier
authors.forename: E
authors.initials: E
authors.lastname: Jokitalo
authors.forename: G
authors.initials: G
authors.lastname: Warren
mesh_terms: D000818:Animals; D020126:Brefeldin A / Q000494:pharmacology; D016827:CD8 Antigens / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D002522:Chlorocebus aethiops; D005089:Exocytosis / Q000502:physiology*; D006056:Golgi Apparatus / Q000502:physiology*; D008562:Membrane Glycoproteins; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D016827:CD8 Antigens; C028391:G protein, vesicular stomatitis virus; D008562:Membrane Glycoproteins; D011500:Protein Synthesis Inhibitors; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins; D020126:Brefeldin A