title: Structure of the O-specific polysaccharide of the bacterium Proteus vulgaris O23.
abstract: An acidic O-specific polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of the bacterium Proteus vulgaris O23 (strain PrK 44/57) and found to contain 2-acetamido-2-deoxy-D-galactose, 2-acetamido-2-deoxy-D-glucose, and D-galacturonic acid. Based on 1H- and 13C-NMR spectroscopic studies, including two-dimensional correlation spectroscopy (COSY), total correlation spectroscopy (TOCSY), nuclear Overhauser effect spectroscopy (NOESY), and 1H,13C heteronuclear multiple-quantum coherence (HMQC) experiments, the following structure of the branched tetrasaccharide repeating unit of the polysaccharide was established: [figure], where the degree of O-acetylation of the terminal GalA residue at position 4 is about 80%. A structural similarity of the O-specific polysaccharides of P. vulgaris O23 and P. mirabilis O23 is discussed.
authors.affiliation: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, 117913, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Perepelov
authors.forename: A S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: D
authors.initials: D
authors.lastname: Babichka
authors.forename: S N
authors.initials: SN
authors.lastname: Senchenkova
authors.forename: B
authors.initials: B
authors.lastname: Bartodziejska
authors.forename: A
authors.initials: A
authors.lastname: Rozalski
authors.forename: Y A
authors.initials: YA
authors.lastname: Knirel
mesh_terms: D000107:Acetylation; D002240:Carbohydrate Sequence; D002849:Chromatography, Gas; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry* / Q000302:isolation & purification; D011134:Polysaccharides / Q000737:chemistry*; D011514:Proteus vulgaris / Q000737:chemistry* / Q000276:immunology
chemical_list: D019081:O Antigens; D011134:Polysaccharides