Separation of lactoferrin-a and -b from bovine colostrum.
Bovine lactoferrin was separated into lactoferrin-a and lactoferrin-b from bovine colostrum. Lactoferrin-a was eluted at 0.38 M NaCl and lactoferrin-b was eluted at 0.43 M NaCl by carboxymethyl cation-exchange chromatography at pH 7.7, 0.05 M phosphate buffer. The molecular weights were estimated at 84,000 for lactoferrin-a and 80,000 for lactoferrin-b. Lactoferrin-a contents were 258.0 mg/L and lactoferrin-b contents were 524.3 mg/L of colostrum for cow 19. From colostrum to normal milk, total lactoferrin was from 17.1 to 129.4 mg/L during the normal lactational period; however, lactoferrin did not separate clearly into lactoferrin-a and lactoferrin-b. The lactoferrin-a measured from six cows was 258.0, 114.0, 112.8, 64.0, 59.7, and 22.4 mg/ L and the lactoferrin-b 524.3, 331.8, 184.7, 170.7, 129.3, and 44.0 mg/L, respectively. The average was 105.2 mg (31.3%) for lactoferrin-a and 230.8 mg (68.7%) for lactoferrin-b.
Yoshida
S
S
Faculty of Applied Biological Science, Dairy Chemistry Laboratory, Hiroshima University, Higashi-Hiroshima, Japan. s.yosida@d6.dion.ne.jp
Wei
Z
Z
Shinmura
Y
Y
Fukunaga
N
N
D000818:Animals; D002417:Cattle; D002845:Chromatography / Q000379:methods / Q000662:veterinary; D003126:Colostrum / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007774:Lactation; D007781:Lactoferrin / Q000032:analysis / Q000302:isolation & purification*; D007782:Lactoglobulins / Q000032:analysis / Q000302:isolation & purification; D008970:Molecular Weight; D012965:Sodium Chloride
D007782:Lactoglobulins; D012965:Sodium Chloride; D007781:Lactoferrin