[High-level expression of BYDV GAV coat protein gene in Escherichia coli].
The IPTG-inducible expression vector containing the BYDV GAV coat protein gene was constructed and transferred into E. coli BL21(DE3). High-level expression of the specific protein was achieved by IPTG induction. The results of SDS-PAGE and Western blotting show that the expression product which accumulates 19.5% of the total cellular proteins estimated by scanning is 24 kD BYDV GAV coat protein plus eleven amino acids of pET-5a.
Chang
S J
SJ
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Chinese Agricultural Academy of Science, Beijing.
Ma
Z H
ZH
Wang
X F
XF
Li
L
L
Zhou
G H
GH
D002213:Capsid / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D001467:Hordeum / Q000821:virology*; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D017793:Luteovirus / Q000235:genetics*
D007544:Isopropyl Thiogalactoside