title: Protective effect of vaccination with culture supernate of M. hyopneumoniae against experimental infection in pigs.
abstract: The protective activity of Mycoplasma hyopneumoniae inactivated vaccine prepared from sedimented whole cells and cell-free culture supernates was evaluated experimentally using hysterectomy-produced, colostrum-deprived pigs in which mycoplasmal pneumonia had been induced. The culture supernate vaccine containing less than 10(1) colour-changing units (CCU)/0.2 ml of M. hyopneumoniae significantly (P < 0.05) reduced the percentage of lung lesions compared to controls (3.2 +/- 3.9 vs. 12.2 +/- 2.2%), whereas the sedimented whole cells vaccine containing 10(10) CCU/0.2 ml of organisms provided variable protection (18.7 +/- 16.5 vs. 12.2 +/- 2.2%). Serum from the pigs vaccinated with culture supernate reacted with six protein bands of 97, 89, 65, 46, 42 and 41 kDa by immunoblot analysis. From these results, we conclude that vaccination with culture supernate of M. hyopneumoniae can provide protection against M. hyopneumoniae infection and that these antigens in the culture supernate may be closely related to the reduction of lung lesions.
authors.affiliation: Zen-noh Institute of Animal Health7 Ohja-machi, Sakura, Chiba, Japan. okadamu@zk.zennoh.or.jp
authors.forename: M
authors.initials: M
authors.lastname: Okada
authors.forename: T
authors.initials: T
authors.lastname: Asai
authors.forename: M
authors.initials: M
authors.lastname: Ono
authors.forename: T
authors.initials: T
authors.lastname: Sakano
authors.forename: S
authors.initials: S
authors.lastname: Sato
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000907:Antibodies, Bacterial / Q000097:blood*; D001428:Bacterial Vaccines; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D015151:Immunoblotting / Q000662:veterinary; D009174:Mycoplasma / Q000276:immunology*; D045729:Pneumonia of Swine, Mycoplasmal / Q000517:prevention & control / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000473:pathology / Q000517:prevention & control*; D014611:Vaccination / Q000662:veterinary*; D015164:Vaccines, Inactivated
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D015164:Vaccines, Inactivated