Jackfruit lectin: properties of mitogenicity and the inhibition of herpesvirus infection.
Jackfruit lectin (JFL) from Artocarpus heterophyllus has been found to exhibit inhibitory activity in vitro with a cytopathic effect towards herpes simplex virus type 2 (HSV-2), varicella-zoster virus (VZV), and cytomegalovirus (CMV). The 50% inhibitory dose values from plaque reduction assay (inactivation) were 2.5, 5, and 10 Eg/ml of JFL for HSV-2, VZV, and CMV, respectively. Lymphocyte proliferation was significantly increased in the presence of the JFL in the concentration range of 2.5 to 50 Eg/ml, but was reduced at 500 Eg/ml. It was found that CD16(+)/CD56(+) cells (natural killer cells) were induced among the primary lymphocyte subpopulations. The activity of natural killer (NK) cells was not affected by JFL in the concentration range of 5 to 500 Eg/ml. These data suggest that JFL is mitogenic for NK lymphocyte (CD16(+)/CD56(+)) and also active against HSV-2, VZV, and CMV.
Wetprasit
N
N
Department of Biotechnology, Faculty of Science, Ramkhamhaeng University, Bangkok 10240, Thailand. nuanwee@yahoo.com
Threesangsri
W
W
Klamklai
N
N
Chulavatnatol
M
M
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006566:Herpesviridae Infections / Q000517:prevention & control*; D018258:Herpesvirus 2, Human / Q000187:drug effects*; D014645:Herpesvirus 3, Human / Q000187:drug effects*; D006801:Humans; D007694:Killer Cells, Natural / Q000187:drug effects / Q000502:physiology; D037102:Lectins / Q000494:pharmacology* / Q000627:therapeutic use; D007928:Lethal Dose 50; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000502:physiology; D037121:Plant Lectins; D010948:Viral Plaque Assay
D000998:Antiviral Agents; D037102:Lectins; D037121:Plant Lectins; C043696:jacalin