Identification of five different Patr class I molecules that bind HLA supertype peptides and definition of their peptide binding motifs.
We have sequenced the Pan troglodytes class I (Patr) molecules from three common chimpanzees and expressed them as single molecules in a class I-deficient cell line. These lines were utilized to obtain purified class I molecules to define the peptide binding motifs associated with five different Patr molecules. Based on these experiments, as well as analysis of the predicted structure of the B and F polymorphic MHC pockets, we classified five Patr molecules (Patr-A*0101, Patr-B*0901, Patr-B*0701, Patr-A*0602, and Patr-B*1301) within previously defined supertype specificities associated with HLA class I molecules (HLA-A3, -B7, -A1, and -A24 supertypes). The overlap in the binding repertoire between specific HLA and Patr class I molecules was in the range of 33 to 92%, depending on the particular Patr molecule as assessed by the binding of HIV-, hepatitis B virus-, and hepatitis C virus-derived epitopes. Finally, live cell binding assays of nine chimpanzee-derived B cell lines demonstrated that HLA supertype peptides bound to Patr class I molecules with frequencies in the 20-50% range.
McKinney
D M
DM
Epimmune Inc., San Diego, CA 92121, USA. dmckinney@epimmune.com
Erickson
A L
AL
Walker
C M
CM
Thimme
R
R
Chisari
F V
FV
Sidney
J
J
Sette
A
A
D000483:Alleles; D020816:Amino Acid Motifs / Q000235:genetics / Q000276:immunology; D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism; D002461:Cell Line, Transformed; D005805:Genes, MHC Class I; D006680:HLA Antigens / Q000378:metabolism*; D015788:HLA-A1 Antigen / Q000235:genetics / Q000378:metabolism; D015791:HLA-A3 Antigen / Q000235:genetics / Q000378:metabolism; D015235:HLA-B Antigens / Q000235:genetics / Q000378:metabolism; D015793:HLA-B7 Antigen / Q000235:genetics / Q000378:metabolism; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D009842:Oligopeptides / Q000138:chemical synthesis / Q000276:immunology* / Q000378:metabolism*; D002679:Pan troglodytes / Q000235:genetics / Q000276:immunology*; D011485:Protein Binding / Q000235:genetics / Q000276:immunology; D017422:Sequence Analysis, DNA; D014162:Transfection
D006680:HLA Antigens; D015788:HLA-A1 Antigen; D015791:HLA-A3 Antigen; D015235:HLA-B Antigens; D015793:HLA-B7 Antigen; D015395:Histocompatibility Antigens Class I; D009842:Oligopeptides