title: Interaction with CBP/p300 enables the bovine papillomavirus type 1 E6 oncoprotein to downregulate CBP/p300-mediated transactivation by p53.
abstract: The E6 oncoprotein of bovine papillomavirus type 1 (BPV-1) can transform cells independently of p53 degradation. The precise mechanisms underlying this transformation are not yet completely understood. Here it is shown that BPV-1 E6 interacts with CBP/p300 in the same way as described for the E6 proteins of oncogenic human papillomaviruses. This interaction results in an inhibition of the transcriptional coactivator function of CBP/p300 required by p53 and probably by other transcription factors. The comparison of the CBP/p300-binding properties of BPV-1 E6 mutants previously characterized in transcription and transformation studies suggests (i) that the E6-CBP/p300 interaction may be necessary, but not sufficient, for cell transformation, and (ii) that the transcriptional activator function, inherent to the E6 protein, is not derived from forming a complex with CBP/p300.
authors.affiliation: Laboratory for Papillomavirus Biology, Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609, Republic of Singapore1.
authors.forename: Holger
authors.initials: H
authors.lastname: Zimmermann
authors.affiliation: Laboratory for Papillomavirus Biology, Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609, Republic of Singapore1.
authors.forename: Choon-Heng
authors.initials: CH
authors.lastname: Koh
authors.affiliation: Laboratory for Papillomavirus Biology, Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609, Republic of Singapore1.
authors.forename: Roland
authors.initials: R
authors.lastname: Degenkolbe
authors.affiliation: Laboratory for Papillomavirus Biology, Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609, Republic of Singapore1.
authors.forename: Mark J
authors.initials: MJ
authors.lastname: O'Connor
authors.affiliation: Institut für Virologie der Universität zu Köln, Cologne, Germany2.
authors.forename: Andreas
authors.initials: A
authors.lastname: Müller
authors.affiliation: Institut für Virologie der Universität zu Köln, Cologne, Germany2.
authors.forename: Gertrud
authors.initials: G
authors.lastname: Steger
authors.affiliation: Department of Dermatology, New England Medical Center, Boston, MA, USA3.
authors.forename: Jason J
authors.initials: JJ
authors.lastname: Chen
authors.affiliation: Department of Dermatology, New England Medical Center, Boston, MA, USA3.
authors.forename: Yun
authors.initials: Y
authors.lastname: Lui
authors.affiliation: Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA 02111, USA4.|Department of Dermatology, New England Medical Center, Boston, MA, USA3.
authors.forename: Elliot
authors.initials: E
authors.lastname: Androphy
authors.affiliation: Laboratory for Papillomavirus Biology, Institute of Molecular and Cell Biology, 30 Medical Drive, Singapore 117609, Republic of Singapore1.
authors.forename: Hans-Ulrich
authors.initials: HU
authors.lastname: Bernard
mesh_terms: D000818:Animals; D052688:Bovine papillomavirus 1 / Q000235:genetics*; D002417:Cattle; D002460:Cell Line; D002472:Cell Transformation, Viral; D015536:Down-Regulation; D015967:Gene Expression Regulation, Viral; D016158:Genes, p53; D006801:Humans; D009687:Nuclear Proteins / Q000235:genetics*; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D015534:Trans-Activators / Q000235:genetics*; D015533:Transcriptional Activation
chemical_list: D009687:Nuclear Proteins; D009856:Oncogene Proteins, Viral; D015534:Trans-Activators; C059269:protein E6, Bovine papillomavirus