Human tissue distribution of TA02, which is homologous with a new type of aspartic proteinase, napsin A.
The N-terminal amino acid sequence of TA02 (molecular weight 35.0 kDa, isoelectric point 5.29), which is associated with primary lung adenocarcinoma, was determined and a fragment peptide was used to generate mouse monoclonal antibodies (mAbs) against TA02. The amino acid sequence suggested that TA02 might be homologous with napsin A, a new type of aspartic proteinase. In this context, we confirmed the expression of napsin A in primary lung adenocarcinoma using reverse-transcription polymerare chain reaction (RT-PCR) and showed that the TA02 mAbs reacted with glutathione-S-transferase (GST)-napsin A fusion protein. We concluded that TA02 is the same molecule as napsin A, and showed immunohistochemically that it is distributed mainly in type II pneumocytes, alveolar macrophages, renal tubules and exocrine glands and ducts in the pancreas. In particular, type II pneumocytes and alveolar macrophages showed high expression of TA02 among human normal tissues. In primary lung adenocarcinoma, 47 out of 58 (81.0%) primary lesions were positive. All well-differentiated adenocarcinomas except those of goblet cell type showed high expression of TA02. In addition, two out of seven (28.6%) large cell carcinomas showed low expression of TA02. The other histopathological types of primary lung cancer did not express TA02 at all. A few cases of renal cell cancer, pancreatic cancer, breast cancer, thyroid cancer, colon cancer and ovarian cancer showed low expression, but the staining patterns were completely different from that of primary lung adenocarcinoma, which showed a granular staining pattern. Our novel mAbs should be valuable for immunochemical detection of TA02/napsin A.
Hirano
T
T
Department of Surgery, Tokyo Medical University, Shinjuku-ku, Tokyo 160-0023, Japan. m-hirano@guitar.ocn.ne.jp
Auer
G
G
Maeda
M
M
Hagiwara
Y
Y
Okada
S
S
Ohira
T
T
Okuzawa
K
K
Fujioka
K
K
Franzén
B
B
Hibi
N
N
Seito
T
T
Ebihara
Y
Y
Kato
H
H
D000230:Adenocarcinoma / Q000378:metabolism*; D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D016282:Aspartic Acid Endopeptidases / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015153:Blotting, Western; D003001:Cloning, Molecular; D004926:Escherichia coli; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000378:metabolism*; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020539:Sequence Analysis, Protein; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D016282:Aspartic Acid Endopeptidases; C498549:NAPSA protein, human