title: Usefulness of (99m)Tc-d,l-HMPAO for estimation of GSH content in tumor tissues.
abstract: To investigate whether [(99m)Tc]-hexamethyl propyleneamine oxime ([(99m)Tc]-HMPAO) is applicable for evaluating glutathione (GSH) localization in tumor, the difference of distribution between [(99m)Tc]-d,l- and meso-HMPAO was studied using a mouse tumor model. Biodistribution of [(99m)Tc]-d,l- or meso-HMPAO was studied in GSH-depleted and control Ehrlich tumor-bearing mice. GSH levels in tumors in GSH-depleted and control mice were measured in another set of mice. The uptake of [(99m)Tc]-d,l-HMPAO in tumor was significantly decreased by the diethyl maleate (DEM) treatment. On the other hand, the DEM treatment increased the accumulation of [(99m)Tc]-meso-HMPAO in tumor. Meanwhile, the content of GSH was lowest in tumor among the tissues tested and decreased in a manner similar to other tissues on preloading of DEM. [(99m)Tc]-d,l-HMPAO may be useful for estimating the GSH status in a certain tumor and thereby contribute to the diagnosis of anticancer therapy.
authors.affiliation: Faculty of Pharmaceutical Sciences, Science University of Tokyo, Tokyo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Shimura
authors.forename: A
authors.initials: A
authors.lastname: Musya
authors.forename: T
authors.initials: T
authors.lastname: Hashimoto
authors.forename: S
authors.initials: S
authors.lastname: Kojima
authors.forename: A
authors.initials: A
authors.lastname: Kubodera
authors.forename: T
authors.initials: T
authors.lastname: Sasaki
mesh_terms: D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging / Q000378:metabolism; D002286:Carcinoma, Ehrlich Tumor / Q000000981:diagnostic imaging / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism*; D007668:Kidney / Q000000981:diagnostic imaging / Q000378:metabolism; D008099:Liver / Q000000981:diagnostic imaging / Q000378:metabolism; D008168:Lung / Q000000981:diagnostic imaging / Q000378:metabolism; D008297:Male; D008298:Maleates / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D009368:Neoplasm Transplantation; D009928:Organ Specificity / Q000187:drug effects; D011877:Radionuclide Imaging; D013154:Spleen / Q000000981:diagnostic imaging / Q000378:metabolism; D013237:Stereoisomerism; D019690:Technetium Tc 99m Exametazime / Q000378:metabolism / Q000493:pharmacokinetics*; D014018:Tissue Distribution
chemical_list: D008298:Maleates; D019690:Technetium Tc 99m Exametazime; C014476:diethyl maleate; D005978:Glutathione