title: Cloning and sequence analysis of a cDNA for an inducible 70 kDa heat shock protein (Hsp70) of the American oyster (Crassostrea virginica).
abstract: We have been investigating 70 kDa heat shock proteins (Hsp70s) as potential molecular markers for improved breeding and stress management to revitalize stocks of the American oyster, C. virginica. From a C. virginica visceral mass library, a 2.2 kb full-length cDNA was isolated that included a 634 amino acid open reading frame possessing approximately 80% sequence identity with inducible and constitutive Hsp70s of a broad array of animal species. Northern blotting indicated that the cloned cDNA preferentially recognized an mRNA of about 2 kb that was virtually absent from visceral mass under basal conditions but greatly increased after in vivo heat shock of American and Pacific oysters, suggesting that the cDNA codes for an inducible Hsp70.
authors.affiliation: Zoology Department, North Carolina State University, Raleigh 27695-7617, USA.
authors.forename: A V
authors.initials: AV
authors.lastname: Rathinam
authors.forename: T T
authors.initials: TT
authors.lastname: Chen
authors.forename: R M
authors.initials: RM
authors.lastname: Grossfeld
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D016384:Consensus Sequence; D017124:Conserved Sequence; D018076:DNA, Complementary; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics*; D006358:Hot Temperature; D008969:Molecular Sequence Data; D010125:Ostreidae / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; D018840:HSP70 Heat-Shock Proteins