Granulocyte-colony stimulating factor induced intranuclear endonuclease in murine leukemia cell line.
We have reported that murine leukemia cell line (C2M-A5) induced apoptosis by G-CSF. To clarify the mechanism, mRNA expression of apoptosis-related genes was studied. It revealed transient over-expression of c-myc, H-ras and p53 and down-expression of bcl-2. These changes were known as triggers of endonuclease induction. After 96 h culture with G-CSF, apoptosis was occurred simultaneously with endonuclease (37 kd) activation. This endonuclease induced the digestion of double-strand DNA and might be associated with caspase3. Although G-CSF accelerates cell growth and prevents apoptosis in general, it is a contradictory effect. We concluded that G-CSF induced endogenous endonuclease activity in C2M-A5.
Handa
A
A
Division of Hematology and Infectious Diseases, Department of Internal Medicine, Saitama Medical School, 350-0451, Saitama, Japan. handaa@nih.gov
Kashimura
T
T
Yamamoto
A
A
Murohashi
I
I
Bessho
M
M
Hirashima
K
K
D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics*; D053148:Caspase 3; D020169:Caspases; D004720:Endonucleases / Q000378:metabolism*; D015870:Gene Expression; D019254:Genes, bcl-2; D016259:Genes, myc; D016158:Genes, p53; D011905:Genes, ras; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D007951:Leukemia, Myeloid / Q000235:genetics / Q000473:pathology*; D051379:Mice; D012333:RNA, Messenger / Q000378:metabolism; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D016179:Granulocyte Colony-Stimulating Factor; D004720:Endonucleases; C505409:Casp3 protein, mouse; D053148:Caspase 3; D020169:Caspases