Effect of hyperinsulinemia on amino acid utilization in the ovine fetus.
We studied the effect of an acute 4-h period of hyperinsulinemia (H) on net utilization rates (AAUR(net)) of 21 amino acids (AA) in 17 studies performed in 13 late-gestation fetal sheep by use of a novel fetal hyperinsulinemic-euglycemic-euaminoacidemic clamp. During H [84 +/- 12 (SE) microU/ml H, 15 +/- 2 microU/ml control (C), P < 0. 00001], euglycemia was maintained by glucose clamp (19 +/- 0.05 micromol/ml H, 1.19 +/- 0.04 micromol/ml C), and euaminoacidemia (mean 4.1 +/- 3.3% increase for all amino acid concentrations [AA], nonsignificantly different from zero) was maintained with a mixed amino acid solution adjusted to keep lysine concentration constant and other [AA] near C values. H produced a 63.7% increase in AAUR(net) (3.29 +/- 0.66 micromol. min(-1). kg(-1) H, 2.01 +/- 0.55 micromol. min(-1). kg(-1) C, P < 0.001), accounting for a 60.1% increase in fetal nitrogen uptake rate (2,064 +/- 108 mg. day(-1). kg(-1) H, 1,289 +/- 73 mg. day(-1). kg(-1) C, P < 0.001). Mean AA clearance rate (AAUR(net)/[AA]) increased by 64.5 +/- 18.9% (P < 0. 001). Thus acute physiological H increases net amino acid and nitrogen utilization rates in the ovine fetus independent of plasma glucose and [AA].
Thureen
P J
PJ
Perinatal Research Center and Departments of Pediatrics, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA. patti.thureen@uchsc.edu
Scheer
B
B
Anderson
S M
SM
Tooze
J A
JA
Young
D A
DA
Hay
W W
WW
D000596:Amino Acids / Q000097:blood / Q000493:pharmacokinetics*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D004573:Electrolytes; D005260:Female; D005333:Fetus / Q000378:metabolism; D005865:Gestational Age; D005947:Glucose; D015309:Glucose Clamp Technique; D006946:Hyperinsulinism / Q000378:metabolism*; D007004:Hypoglycemic Agents / Q000097:blood / Q000494:pharmacology; D007328:Insulin / Q000097:blood / Q000494:pharmacology; D008239:Lysine / Q000097:blood / Q000493:pharmacokinetics; D009584:Nitrogen / Q000493:pharmacokinetics; D057947:Parenteral Nutrition Solutions; D011247:Pregnancy; D012756:Sheep; D012996:Solutions; D014469:Umbilical Arteries / Q000502:physiology
D000596:Amino Acids; D001786:Blood Glucose; D004573:Electrolytes; D007004:Hypoglycemic Agents; D007328:Insulin; D057947:Parenteral Nutrition Solutions; D012996:Solutions; C545824:amino-acid, glucose, and electrolyte solution; D005947:Glucose; D008239:Lysine; D009584:Nitrogen