Evaluation of p-naphtholbenzein-beta-D-galactoside as a substrate for bacterial beta-galactosidase.
We describe the synthesis of a new substrate for the detection of beta-galactosidase and evaluate its performance in comparison with 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) and cyclohexenoesculetinbeta-D-galactoside (CHE-Gal). Of 206 Enterobacteriaceae strains able to hydrolyze X-Gal, 194 (94.2%) hydrolyzed CHE-Gal and 192 (93.2%) hydrolyzed p-naphtholbenzein-beta-D-galactoside (PNB-Gal). We conclude that PNB-Gal is an effective substrate for the detection of beta-galactosidase.
James
A L
AL
Department of Chemical & Life Sciences, University of Northumbria, Newcastle-upon-Tyne, United Kingdom.
Chilvers
K F
KF
Perry
J D
JD
Armstrong
L
L
Gould
F K
FK
D002863:Chromogenic Compounds / Q000138:chemical synthesis; D004755:Enterobacteriaceae / Q000201:enzymology*; D005697:Galactosides / Q000138:chemical synthesis; D007211:Indoles; D009281:Naphthalenes / Q000138:chemical synthesis; D013379:Substrate Specificity; D014468:Umbelliferones; D015008:Yersinia enterocolitica / Q000201:enzymology; D001616:beta-Galactosidase / Q000378:metabolism*
D002863:Chromogenic Compounds; D005697:Galactosides; D007211:Indoles; D009281:Naphthalenes; D014468:Umbelliferones; C101993:cyclohexenoesculetin-beta-galactoside; C419824:p-naphtholbenzeingalactoside; D001616:beta-Galactosidase; C044888:5-bromo-4-chloro-3-indolyl beta-galactoside