Construction and characterization of an Escherichia coli strain genetically engineered for Ni(II) bioaccumulation.
An Escherichia coli strain that accumulated Ni(II) was constructed by introducing the nixA gene (coding for a nickel transport system) from Helicobacter pylori into JM109 cells that expressed a glutathione S-transferase-pea metallothionein fusion protein. The resulting strain accumulated 15 micromol of Ni(II) per g (dry weight) from a 10 microM Ni(II) solution, four times the level taken up by JM109 cells. Ni(II) accumulation did not require an energy source, was inhibited by only 50% by 0.1 M NaCl, and occurred over the pH range from 3 to 9.
Krishnaswamy
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R
Field of Environmental Toxicology, Cornell University, Ithaca, New York 14853, USA.
Wilson
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DB
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001693:Biological Transport, Active; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D027682:Cation Transport Proteins; D004785:Environmental Pollutants / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D005798:Genes, Bacterial; D005818:Genetic Engineering; D016480:Helicobacter pylori / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D009532:Nickel / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D002352:Carrier Proteins; D027682:Cation Transport Proteins; D004785:Environmental Pollutants; D008565:Membrane Proteins; C095257:NixA protein, Helicobacter pylori; D011993:Recombinant Fusion Proteins; D009532:Nickel