title: Application of a non-indexed dual sprayer pneumatically assisted electrospray source to the high throughput quantitation of target compounds in biological fluids.
abstract: The need for increased throughput in the quantitation of target compounds in biological fluids by high performance liquid chromatography/mass spectrometry continues to drive research in this area. This report describes the application of a prototype dual sprayer electrospray source for the quantitative analysis of biological samples. Quantitative performance for 180 compounds in a microsomal stability assay was found to be adequate when compared with a conventional single sprayer measurement. Issues with use of dual sprayers in a routine production environment are discussed.
authors.affiliation: Pfizer Inc., Candidate Enhancement Group, Groton, CT 06340, USA.
authors.forename: D L
authors.initials: DL
authors.lastname: Hiller
authors.forename: A H
authors.initials: AH
authors.lastname: Brockman
authors.forename: L
authors.initials: L
authors.lastname: Goulet
authors.forename: S
authors.initials: S
authors.lastname: Ahmed
authors.forename: R O
authors.initials: RO
authors.lastname: Cole
authors.forename: T
authors.initials: T
authors.lastname: Covey
mesh_terms: D000818:Animals; D001685:Biological Factors / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D006207:Half-Life; D008862:Microsomes, Liver / Q000737:chemistry / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation* / Q000379:methods*
chemical_list: D001685:Biological Factors