Attachment of Toxoplasma gondii to a specific membrane fraction of CHO cells.
We have observed previously that attachment of Toxoplasma gondii to synchronized host cells is considerably increased at the mid-S phase (4 h postrelease). Synchronized CHO host cells at the mid-S phase were fractionated by molecular weight, and the antigens were used to produce a panel of polyclonal mouse antisera. The polyclonal antisera raised against fraction 4 with molecular mass ranging approximately from 18 to 40 kDa significantly reduced attachment to mid-S-phase host cells. Immunofluorescence assays demonstrated strong reactivity to mid-S-phase host cells and identified a number of potential receptors on Western blots. These data indicate that there is a specific host membrane receptor for parasite attachment that is upregulated during the mid-S phase of the host cell cycle.
Dutta
C
C
Department of Medicine and Microbiology, Dartmouth Medical School, Lebanon, New Hampshire 03756, USA.
Grimwood
J
J
Kasper
L H
LH
D000818:Animals; D016466:CHO Cells; D002462:Cell Membrane / Q000469:parasitology*; D006224:Cricetinae; D019084:Fluorescent Antibody Technique, Indirect; D008565:Membrane Proteins / Q000032:analysis / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D011817:Rabbits; D016196:S Phase; D014122:Toxoplasma / Q000502:physiology*
D008565:Membrane Proteins