Sage transcript profiles in cultured human fetal fibroblasts, WI-38.
Serial analysis of gene expression (SAGE) allows us to analyze the profile of genes expressed in human lung fibroblast cell line WI-38. Manual sequencing of approximately 10,000 transcripts derived from WI-38 cells revealed 1025 genes expression among of which 431 tags matched GenBank entries, whereas 594 corresponded to novel previously undescribed transcripts. 7SL RNA (7L7) 5'-truncated pseudogene and the gene with no match (tag: TCCCTAGCT) were found the most abundant among all the analyzed genes (10.2 and 4.6% respectively). The expression pattern of the genes coding for cytoskeletal proteins, extracellular matrix components (ECM) and cytokines has been obtained. The results here demonstrate the unique gene expression pattern in human WI-38 fibroblast cell line and support a possibility of useful application of SAGE method for differential expression analysis.
Shklyaev
S
S
Department of Nature Medicine, Atmomic Bomb Disease Institute, Nagasaki University School of Medicine, Japan.
Namba
H
H
Hara
T
T
Ohtsuru
A
A
Yamashita
S
S
D020121:5' Untranslated Regions / Q000235:genetics; D002460:Cell Line; D016207:Cytokines / Q000235:genetics*; D003598:Cytoskeletal Proteins / Q000235:genetics*; D016326:Extracellular Matrix Proteins / Q000235:genetics*; D005333:Fetus; D005347:Fibroblasts; D006801:Humans; D008168:Lung; D011544:Pseudogenes; D014158:Transcription, Genetic
D020121:5' Untranslated Regions; D016207:Cytokines; D003598:Cytoskeletal Proteins; D016326:Extracellular Matrix Proteins