title: The genomic sequence of a contemporary wild-type mumps virus strain.
abstract: Vaccination has the potential to eradicate mumps, and 82 countries now include a live attenuated mumps vaccine as part of their childhood vaccination programme. Although, monotypic, genetic variants of mumps virus (MuV) have been described based on comparison of the SH gene sequences, and at least seven genotypes have been identified. We now report the entire sequence of a recently isolated wild type MuV strain, Glouc1/UK96 (Glouc1) by direct sequencing of the cDNA obtained from cell culture fluid. The genome of this recent isolate was 15384 nucleotides in length. There were 579 nucleotide differences (3.8%) and 71 amino acid differences (1.5%) between Glouc1, a genotype G strain and Ur-AM9, a genotype B strain. Other MuV strains with available sequences were also compared with this pathological strain. The sequence of the contemporary strain reported here provides a picture of the variability of MuV over its entire genome (GenBank accession no. AF280799).
authors.affiliation: Enteric, Respiratory and Neurological Virus Laboratory, Central Public Health Laboratory, NW9 5HT, London, UK. ljin@phls.nhs.uk
authors.forename: L
authors.initials: L
authors.lastname: Jin
authors.forename: S
authors.initials: S
authors.lastname: Beard
authors.forename: A
authors.initials: A
authors.lastname: Hale
authors.forename: W
authors.initials: W
authors.lastname: Knowles
authors.forename: D W
authors.initials: DW
authors.lastname: Brown
mesh_terms: D005814:Genes, Viral; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D009109:Mumps virus / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis; D017422:Sequence Analysis, DNA
chemical_list: D012367:RNA, Viral