title: Protein tyrosine kinase inhibitor, genistein, enhances apoptosis and cell cycle arrest in K562 cells treated with gamma-irradiation.
abstract: Genistein, is a natural isoflavone compound with a potent activity against protein tyrosine kinases. The leukemic cell line, K562, is a bcr/abl (Philadelphia chromosome) positive cell line that is resistant to DNA-damaging agents, including gamma-irradiation. Treatment with genistein increased apoptosis and promoted G2-phase arrest in the non-apoptotic population of the gamma-irradiated K562 cells. Irradiated cells that survived 72 h after the irradiation had a normal distribution in cell cycle, whilst genistein treatment kept cells arrested in the G2-phase, decreased the S-phase fraction and suppressed DNA-synthesis. Taken together, our results show that genistein augments apoptotic cell death after gamma-irradiation in K562 cells and this result cannot be attributed to abrogation of the G2/M checkpoint.
authors.affiliation: Theagenion Cancer Hospital, 54007, Thessaloniki, Greece. paptsi@med.auth.gr
authors.forename: K T
authors.initials: KT
authors.lastname: Papazisis
authors.forename: D
authors.initials: D
authors.lastname: Zambouli
authors.forename: O T
authors.initials: OT
authors.lastname: Kimoundri
authors.forename: E S
authors.initials: ES
authors.lastname: Papadakis
authors.forename: V
authors.initials: V
authors.lastname: Vala
authors.forename: G D
authors.initials: GD
authors.lastname: Geromichalos
authors.forename: S
authors.initials: S
authors.lastname: Voyatzi
authors.forename: D
authors.initials: D
authors.lastname: Markala
authors.forename: E
authors.initials: E
authors.lastname: Destouni
authors.forename: L
authors.initials: L
authors.lastname: Boutis
authors.forename: A H
authors.initials: AH
authors.lastname: Kortsaris
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects*; D016203:CDC2 Protein Kinase / Q000378:metabolism; D002452:Cell Count; D002453:Cell Cycle / Q000187:drug effects / Q000528:radiation effects*; D016923:Cell Death / Q000187:drug effects / Q000528:radiation effects; D019926:Cyclin B / Q000378:metabolism; D056744:Cyclin B1; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins / Q000378:metabolism; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004307:Dose-Response Relationship, Radiation; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D016195:G2 Phase; D005720:Gamma Rays; D019833:Genistein / Q000494:pharmacology*; D006801:Humans; D020014:K562 Cells; D008938:Mitosis; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors*; D013997:Time Factors; D014343:Trypan Blue
chemical_list: C534699:CCNB1 protein, human; C493956:CDKN1A protein, human; D019926:Cyclin B; D056744:Cyclin B1; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins; D004791:Enzyme Inhibitors; D004247:DNA; D019833:Genistein; D011505:Protein-Tyrosine Kinases; D016203:CDC2 Protein Kinase; D014343:Trypan Blue