Identification and characterization of 5' extension of mammalian agrin cDNA, the exons and the promoter sequences.
Agrin, which is secreted from motor neurons, is essential for the formation and maintenance of the vertebrate neuromuscular junctions. Here we show the complete N-terminal sequence of the mammalian cDNA required for the expression and secretion as well as the intron/exon structure and the 5'-flanking sequence required for basal promoter activity. The 5'-flanking region and the first exon are extremely GC rich and contain a CpG island. These features may account for hindrance in identification of the 5' end of the cDNA and the promoter region of the mammalian agrin gene.
Shigemoto
K
K
Department of Hygiene, School of Medicine, Ehime University, Japan. shigemot@m.ehime-u.ac.jp
Kubo
S
S
Maruyama
N
N
Yamada
S
S
Obata
K
K
Kikuchi
K
K
Kondo
I
I
D018171:Agrin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002645:Chickens / Q000235:genetics; D003001:Cloning, Molecular; D018387:Codon, Initiator / Q000235:genetics; D018899:CpG Islands / Q000235:genetics; D005091:Exons / Q000235:genetics*; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D006801:Humans; D007438:Introns / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D023282:Nuclease Protection Assays; D011401:Promoter Regions, Genetic / Q000235:genetics*; D022821:RNA Splice Sites / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D020218:Response Elements / Q000235:genetics; D016415:Sequence Alignment; D017384:Sequence Deletion / Q000235:genetics
D018171:Agrin; D018387:Codon, Initiator; D022821:RNA Splice Sites; D012333:RNA, Messenger