Identification of an Arabidopsis thaliana mutant accumulating threonine resulting from mutation in a new dihydrodipicolinate synthase gene.
A novel Arabidopsis DHDPS gene named DHDPS2 was found through identification of a mutant by promoter trapping. The mutation promotes a reduction of growth resulting from combination of a defect in lysine biosynthesis and accumulation of a toxic level of threonine or derived products. The mutant also modifies the amino acid composition issuing from the pyruvate and aspartate pathways, affecting mainly the root compartment. These data are in accordance with the expression of DHDPS2 in the root apex as visualized by expression of the GUS reporter gene. This suggests that a large proportion of the amino acids derived from pyruvate and aspartate are synthesized in this organ.
Sarrobert
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Laboratoire du Métabolisme Carboné, UMR 163, CNRS-CEA, DSV.DEVM, CEA/Cadarache, F-13108 St Paul les Durance Cedex, France.
Thibaud
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MC
Contard-David
P
P
Gineste
S
S
Bechtold
N
N
Robaglia
C
C
Nussaume
L
L
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D017343:Genes, Plant; D006836:Hydro-Lyases / Q000235:genetics*; D008239:Lysine / Q000494:pharmacology; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D018517:Plant Roots / Q000201:enzymology; D030821:Plants, Genetically Modified; D017386:Sequence Homology, Amino Acid; D013912:Threonine / Q000378:metabolism*
D004269:DNA, Bacterial; D018744:DNA, Plant; C036483:T-DNA; D013912:Threonine; D006836:Hydro-Lyases; C020040:4-hydroxy-tetrahydrodipicolinate synthase; D008239:Lysine