title: Morphological changes and expression of protein kinase CK2 beta subunit in the microglia after hypoglossal nerve transection.
abstract: Following hypoglossal nerve transection, the microglia of the rat hypoglossal nucleus expressed protein kinase CK2 beta subunit immunoreactivity. CK2 beta immunostaining occurred on the operated side from postoperative day 3; on day 5 we observed strong immunoreactivity and the immunopositive microglial cell processes surrounded the injured neurones. Thereafter, the immunoreactivity decreased gradually and on day 10 the immunopositive cells surrounded only a few injured neurones. Electron microscopic observations on the hypoglossal nucleus revealed microglia-neuronal contact within 3 hours of nerve injury, and by day 3 all the injured neurones were in contact with microglial cells. These observations indicated that microglia-neuronal contact occurred earlier than the CK2 beta subunit immunoreactivity. CK2 may not be implicated during the initial migration of the microglia to the injured neurones; however, it may enhance the growth and elongation of the microglial cell processes around the injured neurones.
authors.affiliation: Department of Anatomy/Histology, Muhimbili University College of Health Sciences, Dar-Es-salaam, Tanzania.
authors.forename: A P
authors.initials: AP
authors.lastname: Gesase
authors.forename: H
authors.initials: H
authors.lastname: Kiyama
mesh_terms: D000818:Animals; D019771:Axotomy; D047390:Casein Kinase II; D007002:Hypoglossal Nerve / Q000737:chemistry / Q000166:cytology* / Q000601:surgery; D008297:Male; D017628:Microglia / Q000737:chemistry / Q000201:enzymology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D009474:Neurons / Q000648:ultrastructure; D017346:Protein Serine-Threonine Kinases / Q000032:analysis / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases