Anomalous dystroglycan in carcinoma cell lines.
Dystroglycan is a receptor responsible for crucial interactions between extracellular matrix and cytoplasmic space. We provide the first evidence that dystroglycan is truncated. In HC11 normal murine and the 184B5 non-tumorigenic mammary human cell lines, the expected beta-dystroglycan 43 kDa band was found but human breast T47D, BT549, MCF7, colon HT29, HCT116, SW620, prostate DU145 and cervical HeLa cancer cells expressed an anomalous approximately 31 kDa beta-dystroglycan band. alpha-Dystroglycan was udetectable in most of the cell lines in which beta-dystroglycan was found as a approximately 31 kDa species. An anomalous approximately 31 kDa beta-dystroglycan band was also observed in N-methyl-N-nitrosurea-induced primary rat mammary tumours. Reverse transcriptase polymerase chain reaction experiments confirmed the absence of alternative splicing events and/or expression of eventual dystroglycan isoforms. Using protein extraction procedures at low- and high-ionic strength, we demonstrated that both the 43 kDa and approximately 31 kDa beta-dystroglycan bands harbour their transmembrane segment.
Losasso
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Centro Chimica dei Recettori (CNR), Istituto di Chimica e Chimica Clinica, Università Cattolica del Sacro Cuore, Rome, Italy.
Di Tommaso
F
F
Sgambato
A
A
Ardito
R
R
Cittadini
A
A
Giardina
B
B
Petrucci
T C
TC
Brancaccio
A
A
D000818:Animals; D001940:Breast / Q000166:cytology / Q000378:metabolism; D001943:Breast Neoplasms; D002460:Cell Line; D003110:Colonic Neoplasms; D003598:Cytoskeletal Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D049030:Dystroglycans; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D005260:Female; D006367:HeLa Cells; D006801:Humans; D008297:Male; D008321:Mammary Glands, Animal / Q000166:cytology / Q000378:metabolism; D008325:Mammary Neoplasms, Experimental; D008562:Membrane Glycoproteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D011471:Prostatic Neoplasms; D011817:Rabbits; D051381:Rats; D017474:Receptors, Laminin / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D003598:Cytoskeletal Proteins; C483081:DAG1 protein, human; D008562:Membrane Glycoproteins; D017474:Receptors, Laminin; D049030:Dystroglycans