Enantiomeric separation by cyclodextrin modified capillary zone electrophoresis (CD-CZE) of quaternary tetrahydroprotoberberine alkaloids.
A cyclodextrin modified capillary zone electrophoresis (CD-CZE) for the enantiomeric separation of tetrahydroprotoberberine N-metho salts was established. The resolution was optimized by changing the concentration of the electrolyte solution, hydroxypropyl-beta-CD (0.02 M, 0.07 M, or 0.14 M) or dimethyl-beta-CD (0.05 M or 0.15 M) in phosphate buffer (pH 2.5 or 3) containing 10% acetonitrile with an applied voltage of 20 kV. This method was applied toward the enantioselective bio-conversion of quaternary tetrahydroprotoberberine N-metho salts in cultured cells of Corydalis species.
Koyama
J
J
Kobe Pharmaceutical University, Japan. j-koyama@kobepharma-u.ac.jp
Morita
I
I
Kino
A
A
Iwasa
K
K
Tagahara
K
K
D001599:Berberine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification*; D002478:Cells, Cultured; D003505:Cyclodextrins; D019075:Electrophoresis, Capillary; D007202:Indicators and Reagents; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D003505:Cyclodextrins; D007202:Indicators and Reagents; D001599:Berberine