title: Two new biologically active triterpene saponins from Acanthophyllum squarrosum.
abstract: Two novel triterpenoid saponins (1 and 2) have been isolated from the roots of Acanthophyllum squarrosum. The structures were established mainly by a combination of 2D NMR techniques as 3-O-beta-D-galactopyranosyl-(1-->2)-[beta-D-xylopyranosyl-(1-->3)]-be ta-D-glucuronopyranosylgypsogenin-28-O-beta-D-xylopyranosyl-(1-->3 )-b eta-D-xylopyranosyl-(1-->4)-alpha-L-rhamnopyranosyl-(1-->4)-[alpha-L- rhamnopyranosyl-(1-->3)]-beta-D-fucopyranoside (1) and 3-O-beta-D-glucopyranosylgypsogenin-28-O-alpha-L-rhamnopyranosyl-( 1-- >2)-alpha-L-arabinopyranosyl-(1-->2)-[beta-D-glucopyranosyl-(1-->6 )]- beta-D-glucopyranoside (2). Compound 1 showed a moderate concentration-dependent immunomodulatory effect in an in vitro lymphocyte proliferation assay.
authors.affiliation: Laboratoire de Pharmacognosie, Unité MIB, Faculté de Pharmacie, Université de Bourgogne, 7 Bd Jeanne d'Arc, BP 87900, 21079 Dijon Cedex, France.
authors.forename: G
authors.initials: G
authors.lastname: Gaidi
authors.forename: T
authors.initials: T
authors.lastname: Miyamoto
authors.forename: A
authors.initials: A
authors.lastname: Rustaiyan
authors.forename: V
authors.initials: V
authors.lastname: Laurens
authors.forename: M A
authors.initials: MA
authors.lastname: Lacaille-Dubois
mesh_terms: D000276:Adjuvants, Immunologic / Q000302:isolation & purification* / Q000494:pharmacology; D002240:Carbohydrate Sequence; D002455:Cell Division / Q000187:drug effects; D006868:Hydrolysis; D008214:Lymphocytes / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000302:isolation & purification* / Q000494:pharmacology; D010936:Plant Extracts / Q000032:analysis; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D012503:Saponins / Q000302:isolation & purification* / Q000494:pharmacology; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013055:Spectrophotometry, Infrared
chemical_list: C418442:3-O-beta-D-glucopyranosyl-gypsogenin-28-O-alpha-L-rhamnopyranosyl-(1- 2)-alpha-L-arabinopyranosyl-(1-2)-(beta-D-glucopyranosyl-(1-6))-beta-D-glucopyranoside; C418410:3-O-beta-galactopyranosyl-(1-2)-(beta-D-xylopyranosyl-(1-3))-beta-D-glucuronopyranosyl-gypsogenin-28-O-beta-D-xylopyranosyl-(1-3 )-beta-D-xylopyranosyl-(1-4)-alpha-L-rhamnopyranosyl-(1-4)-(alpha-L-rhamnopyranosyl-(1-3))-beta-D-fucopyranoside; D000276:Adjuvants, Immunologic; D010936:Plant Extracts; D012503:Saponins; D009828:Oleanolic Acid