title: Comparison of different protocols of MRC5 cell line permeabilization for detection of CMV IE antigens by flow cytometry.
abstract: The usefulness of four different protocols of permeabilizing human fibroblast cells for detection of CMV immediate-early antigens by flow cytometry was investigated. Permeabilizing protocols employed methanol, methanol/acetone, paraformaldehyde/Triton and FACSPermeabilizing Solution (Becton Dickinson). The best parameters (separation between positive stained CMV infected cells and negative control and detection of infected cells) were achieved for paraformaldehyde/Triton and FACSPermeabilizing Solution (Becton Dickinson).
authors.affiliation: Department of Virology, National Institute of Hygiene, Warsaw, Chocimska 24, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Siennicka
mesh_terms: D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D003587:Cytomegalovirus / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D017874:Immediate-Early Proteins / Q000032:analysis*; D010539:Permeability
chemical_list: D000956:Antigens, Viral; D017874:Immediate-Early Proteins; C044582:immediate-early proteins, cytomegalovirus