Characterization of amiodarone metabolites and impurities using liquid chromatography/atmospheric pressure chemical ionization mass spectrometry.
Using the high performance liquid chromatography/atmospheric pressure chemical ionization tandem mass spectrometry (HPLC/APCI-MS/MS) technique, together with established trends from the literature, the structures of metabolites and impurities of amiodarone, an anti-arrhythmic drug, have been assigned. By comparing analyses of products of incubation with rat liver microsomes with controls in which glucose 6-phosphate dehydrogenase was omitted, metabolites could be distinguished from impurities. Structures for the two proposed metabolites and four impurities are proposed.
Myung
S W
SW
Doping Control Center, Korea Institute of Science and Technology, Seoul. swmyung@kist.re.kr
Chang
Y J
YJ
Min
H K
HK
Kim
D H
DH
Kim
M
M
Kang
T K
TK
Yoo
E A
EA
Sohn
Y T
YT
Yim
Y H
YH
D000638:Amiodarone / Q000031:analogs & derivatives / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D001274:Atmospheric Pressure; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D004048:Diethylamines / Q000032:analysis / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods*; D008862:Microsomes, Liver / Q000378:metabolism; D015394:Molecular Structure; D051381:Rats
D000889:Anti-Arrhythmia Agents; D004048:Diethylamines; C034281:diethylamine; D005954:Glucosephosphate Dehydrogenase; C036116:desethylamiodarone; D000638:Amiodarone