title: Heparan sulfate proteoglycans are critical for the organization of the extracellular distribution of Wingless.
abstract: Recent studies in Drosophila have shown that heparan sulfate proteoglycans (HSPGs) are required for Wingless (Wg/Wnt) signaling. In addition, genetic and phenotypic analyses have implicated the glypican gene dally in this process. Here, we report the identification of another Drosophila glypican gene, dally-like (dly) and show that it is also involved in Wg signaling. Inhibition of dly gene activity implicates a function for DLY in Wg reception and we show that overexpression of DLY leads to an accumulation of extracellular Wg. We propose that DLY plays a role in the extracellular distribution of Wg. Consistent with this model, a dramatic decrease of extracellular Wg was detected in clones of cells that are deficient in proper glycosaminoglycan biosynthesis. We conclude that HSPGs play an important role in organizing the extracellular distribution of Wg.
authors.affiliation: Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: G H
authors.initials: GH
authors.lastname: Baeg
authors.forename: X
authors.initials: X
authors.lastname: Lin
authors.forename: N
authors.initials: N
authors.lastname: Khare
authors.forename: S
authors.initials: S
authors.lastname: Baumgartner
authors.forename: N
authors.initials: N
authors.lastname: Perrimon
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004330:Drosophila / Q000502:physiology*; D029721:Drosophila Proteins; D005786:Gene Expression Regulation; D017344:Genes, Insect; D019812:Heparan Sulfate Proteoglycans / Q000502:physiology*; D008969:Molecular Sequence Data; D011518:Proto-Oncogene Proteins / Q000502:physiology*; D012097:Repressor Proteins / Q000502:physiology; D015398:Signal Transduction; D051155:Wnt1 Protein
chemical_list: D029721:Drosophila Proteins; D019812:Heparan Sulfate Proteoglycans; D011518:Proto-Oncogene Proteins; D012097:Repressor Proteins; D051155:Wnt1 Protein; C074622:wg protein, Drosophila