title: Sex determination by simultaneous amplification of equine SRY and amelogenin genes.
abstract: A quick method for sex determination of horses was developed. Simultaneous amplification of the equine sex-determining region of the Y chromosome gene (SRY) and amelogenin gene (AMEL) accomplished the determination of the presence of both the Y chromosome and SRY gene. In agarose gel electrophoresis, a normal stallion showed 1 SRY band and 3 AMEL (AMELX, AMELY, and AMELX/AMELY heteroduplex) bands, and a normal mare showed a single AMELX band. In XY-mares, 3 AMEL bands were detected as in a normal stallion, but no SRY band. The present method enables a quick diagnosis for XY-mare prior to cytogenetic analysis.
authors.affiliation: Laboratory of Molecular and Cellular Biology, Equine Research Institute, Japan Racing Association, Utsunomiya.
authors.forename: T
authors.initials: T
authors.lastname: Hasegaw
authors.forename: F
authors.initials: F
authors.lastname: Sato
authors.forename: N
authors.initials: N
authors.lastname: Ishida
authors.forename: Y
authors.initials: Y
authors.lastname: Fukushima
authors.forename: H
authors.initials: H
authors.lastname: Mukoyama
mesh_terms: D053523:Amelogenin; D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics*; D003746:Dental Enamel Proteins / Q000235:genetics*; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D005260:Female; D006736:Horses / Q000235:genetics*; D008297:Male; D009687:Nuclear Proteins; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012732:Sex Determination Analysis / Q000662:veterinary*; D019849:Sex Determination Processes; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors; D014960:X Chromosome; D014998:Y Chromosome
chemical_list: D053523:Amelogenin; D004268:DNA-Binding Proteins; D003746:Dental Enamel Proteins; D009687:Nuclear Proteins; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors