title: Human papillomavirus type 16 E5 protein localizes to the Golgi apparatus but does not grossly affect cellular glycosylation.
abstract: The E5 protein of papillomaviruses is a strongly hydrophobic membrane protein that can associate with the 16 kDa protein subunit of the vacuolar proton ATPase in endosomes and the Golgi apparatus resulting in raise of intraorganelle pH. We demonstrate that E5 of human papillomavirus type 16 (HPV16) when transfected into human keratinocytes localizes to the Golgi. Using FACS analysis and western blotting with a variety of lectins as well as analysing the sialylation status of a specific cell surface glycoprotein CD95 (APO-1/Fas), we show that HPV16 E5 does not grossly affect cellular glycosylation, a main Golgi function.
authors.affiliation: Angewandte Tumorvirologie, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Oetke
authors.forename: E
authors.initials: E
authors.lastname: Auvinen
authors.forename: M
authors.initials: M
authors.lastname: Pawlita
authors.forename: A
authors.initials: A
authors.lastname: Alonso
mesh_terms: D015153:Blotting, Western; D002460:Cell Line; D005434:Flow Cytometry; D006031:Glycosylation; D006056:Golgi Apparatus / Q000378:metabolism*; D049452:Green Fluorescent Proteins; D006801:Humans; D015603:Keratinocytes; D037102:Lectins / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000378:metabolism*; D027383:Papillomaviridae / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014162:Transfection
chemical_list: D037102:Lectins; D008164:Luminescent Proteins; D009856:Oncogene Proteins, Viral; D011993:Recombinant Fusion Proteins; C054304:oncogene protein E5, Bovine papillomavirus type 1; D049452:Green Fluorescent Proteins