Ubiquity of the St chloroplast genome in St-containing Triticeae polyploids.
Interspecific hybridization occurs between Tritceae species in the grass family (Poaceae) giving rise to allopolyploid species. To examine bias in cytoplasmic DNA inheritance in these hybridizations, the sequence of the 3' end of the chloroplast ndhF gene was compared among 29 allopolyploid Triticeae species containing the St nuclear genome in combination with the H, I, Ns, P, W, Y, and Xm nuclear genomes. These ndhF sequences were also compared with those from diploid or allotetraploid Triticeae species having the H, I, Ns, P, W, St, and Xm genomes. The cpDNA sequences were highly similar among diploid, allotetraploid, allohexaploid, and allooctoploid Triticeae accessions containing the St nuclear genome, with 0-6-nucleotide (nt) substitutions (0-0.8%) occurring between pairs of species. Neighbor-joining analysis of the sequences showed that the ndhF DNA sequences from species containing the St nuclear genome formed a strongly supported clade. The data indicated a strong preference for cpDNA inheritance from the St nuclear genome-containing parent in hybridizations between Triticeae species. This preference was independent of the presence of the H, I, Ns, P, W, and Xm nuclear genomes, the geographic distribution of the species, and the mode of reproduction. The data suggests that hybridizations having the St-containing parent as the female may be more successful.
Redinbaugh
M G
MG
United States Department of Agriculture, Corn and Soybean Research and Department of Plant Pathology, Ohio Agriculture Research and Development Center, Ohio State University, Wooster 44691, USA. redinbaugh.2@osu.edu
Jones
T A
TA
Zhang
Y
Y
D002736:Chloroplasts / Q000235:genetics*; D014644:Genetic Variation; D018745:Genome, Plant; D009245:NADH Dehydrogenase / Q000235:genetics; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics; D006109:Poaceae / Q000235:genetics*; D011123:Polyploidy; D017422:Sequence Analysis, DNA
D010940:Plant Proteins; C489904:NdhF protein, plant; D009245:NADH Dehydrogenase