title: Cloning of Plasmodium falciparum protein disulfide isomerase homologue by affinity purification using the antiplasmodial inhibitor 1,4-bis[3-[N-(cyclohexyl methyl)amino]propyl]piperazine..
abstract: A series of 10 1,4-bis(3-aminopropyl)piperazine compounds was found to display antiplasmodial activity with 50% growth inhibition between 30 and 250 nM, on three Plasmodium falciparum strains differently sensitive to chloroquine. By affinity chromatography using one of these compounds, a 52-kDa protein was isolated from P. falciparum, microsequenced and cloned. It corresponded to a single copy gene encoding a 453 amino acid protein displaying the typical features of protein disulfide isomerases, a thiol metabolizing enzyme belonging to the thiol: disulfide oxidoreductase superfamily, which was not previously described in malarial species.
authors.affiliation: Laboratoire de Biologie et Evolution des Parasites, FR CNRS 63, Muséum National d'Histoire Naturelle, Paris, France.
authors.forename: I
authors.initials: I
authors.lastname: Florent
authors.forename: E
authors.initials: E
authors.lastname: Mouray
authors.forename: F
authors.initials: F
authors.lastname: Dali Ali
authors.forename: H
authors.initials: H
authors.lastname: Drobecq
authors.forename: S
authors.initials: S
authors.lastname: Girault
authors.forename: J
authors.initials: J
authors.lastname: Schrével
authors.forename: C
authors.initials: C
authors.lastname: Sergheraert
authors.forename: P
authors.initials: P
authors.lastname: Grellier
authors.forename: I
authors.initials: I
authors.lastname: Florenta
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000981:Antiprotozoal Agents; D001483:Base Sequence; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003001:Cloning, Molecular; D003105:Colombia; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010963:Plasmodium falciparum / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D019704:Protein Disulfide-Isomerases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013636:Tanzania; D013785:Thailand
chemical_list: D000981:Antiprotozoal Agents; D011994:Recombinant Proteins; D019704:Protein Disulfide-Isomerases