title: [Maternal serum cytokine levels in pregnancies complicated by PROM].
abstract: OBJECTIVE
  The aim of the study was to evaluate the maternal serum cytokines levels in pregnancies complicated by premature rupture of membranes (PROM).
  
  
  METHODS
  Maternal serum of IL-1 beta, IL-4, IL-6, IL-8 and TNF-alfa levels were assessed in patients with PROM between 24-34 weeks of pregnancy (n = 45). Control group consisted of healthy pregnant women (n = 41) at 24-34 weeks of gestation. Serum cytokines concentrations were measured by commercial available enzyme-linked immunosorbent assays. C-reactive protein level and WBC were estimated in both groups.
  
  
  RESULTS
  Compared to healthy pregnant, the group of patients with PROM had significantly higher serum levels of IL-1 beta (0.76 pg/ml vs 0.41 pg/ml, p = 0.022), TNF-alfa (1332.46 pg/ml vs 58.01 pg/ml, p < 0.00001) and IL-8 (15.79 pg/ml vs 0 pg/ml, p < 0.00001). CRP concentration and WBC were also significantly higher in serum of pregnant women with PROM then in healthy ones (CRP: 10 mg/l vs 0 mg/l, p = 0.043; WBC: 13,188 +/- 3625/mm3 vs 9132 +/- 1913/mm3, p < 0.00001). No significant differences in IL-6 and IL-4 levels were found between groups.
  
  
  CONCLUSIONS
  Differences in serum maternal levels of cytokines between patients with premature ruptures of membranes and healthy pregnant women suggest that reasons and/or consequences of PROM results in changes in immunological system.
authors.affiliation: Kliniki Połoznictwa i Perinatologii II Katedry Połoznictwa i Chorób Kobiecych AM w Lublinie.
authors.forename: E
authors.initials: E
authors.lastname: Poniedziałek-Czajkowska
authors.forename: B
authors.initials: B
authors.lastname: Leszczyńska-Gorzelak
authors.forename: J
authors.initials: J
authors.lastname: Oleszczuk
mesh_terms: D000328:Adult; D016207:Cytokines / Q000097:blood*; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000097:blood*; D006801:Humans; D011247:Pregnancy
chemical_list: D016207:Cytokines