title: Role of mitochondria in ultraviolet-induced oxidative stress.
abstract: The biological effects of ultraviolet radiation (UV), such as DNA damage, mutagenesis, cellular aging, and carcinogenesis, are in part mediated by reactive oxygen species (ROS). The major intracellular ROS intermediate is hydrogen peroxide, which is synthesized from superoxide anion ((*)O(2)(-)) and further metabolized into the highly reactive hydroxyl radical. In this study, we examined the involvement of mitochondria in the UV-induced H(2)O(2) accumulation in a keratinocyte cell line HaCaT. Respiratory chain blockers (cyanide-p-trifluoromethoxy-phenylhydrazone and oligomycin) and the complex II inhibitor (theonyltrifluoroacetone) prevented H(2)O(2) accumulation after UV. Antimycin A that inhibits electron flow from mitochondrial complex III to complex IV increased the UV-induced H(2)O(2) synthesis. The same effect was seen after incubation with rotenone, which blocks electron flow from NADH-reductase (complex I) to ubiquinone. UV irradiation did not affect mitochondrial transmembrane potential (DeltaPsi(m)). These data indicate that UV-induced ROS are produced at complex III via complex II (succinate-Q-reductase).
authors.affiliation: Department of Dermatology, Copenhagen University Hospital, Bispebjerg, Copenhagen, Denmark. rgniadecki@hotmail.com
authors.forename: R
authors.initials: R
authors.lastname: Gniadecki
authors.forename: T
authors.initials: T
authors.lastname: Thorn
authors.forename: J
authors.initials: J
authors.lastname: Vicanova
authors.forename: A
authors.initials: A
authors.lastname: Petersen
authors.forename: H C
authors.initials: HC
authors.lastname: Wulf
mesh_terms: D002460:Cell Line; D006861:Hydrogen Peroxide / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D018384:Oxidative Stress; D017382:Reactive Oxygen Species; D014466:Ultraviolet Rays
chemical_list: D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide