title: Simultaneous up-regulation of urokinase-type plasminogen activator (uPA) and uPA receptor by hepatocyte growth factor/scatter factor in human glioma cells.
abstract: Several lines of evidence indicate that hepatocyte growth factor/scatter factor (HGF/SF) and its receptor, c-Met, may play an important role in progression of human glioma. In this study, effects of HGF/SF on urokinase- type plasminogen activator (uPA)-mediated proteolysis network were examined in c-Met-positive human glioma cell lines. Treatment of the glioma cells with various concentrations of HGF/SF resulted in an enhanced secretion of uPA proteins accompanying increased transcription of uPA mRNA in a dose dependent fashion. The levels of uPA receptor (uPAR) mRNAs were also elevated simultaneously upon HGF/SF stimulation, and the cell-surface associated uPA activity was also elevated by the treatment. Since concomitant expression of HGF and its receptor c-Met are frequently observed in malignant gliomas, these results suggest that HGF/SF participates in invasive process of malignant glioma cells not only by its motility-stimulating activity but also through enhanced degradation of the extracellular matrix induced by autocrine activation of uPA proteolysis network.
authors.affiliation: Department of Neurosurgery, Miyazaki Medical College, Kiyotake, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Moriyama
authors.forename: H
authors.initials: H
authors.lastname: Kataoka
authors.forename: R
authors.initials: R
authors.lastname: Hamasuna
authors.forename: E
authors.initials: E
authors.lastname: Yoshida
authors.forename: T
authors.initials: T
authors.lastname: Sameshima
authors.forename: T
authors.initials: T
authors.lastname: Iseda
authors.forename: K
authors.initials: K
authors.lastname: Yokogami
authors.forename: S
authors.initials: S
authors.lastname: Nakano
authors.forename: M
authors.initials: M
authors.lastname: Koono
authors.forename: S
authors.initials: S
authors.lastname: Wakisaka
mesh_terms: D016543:Central Nervous System Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D005910:Glioma / Q000188:drug therapy / Q000378:metabolism*; D017228:Hepatocyte Growth Factor / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D009842:Oligopeptides / Q000378:metabolism; D019859:Proto-Oncogene Proteins c-met / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D055293:Receptors, Urokinase Plasminogen Activator; D014407:Tumor Cells, Cultured; D015854:Up-Regulation; D014568:Urokinase-Type Plasminogen Activator / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*
chemical_list: D009842:Oligopeptides; C524898:PLAUR protein, human; D011956:Receptors, Cell Surface; D055293:Receptors, Urokinase Plasminogen Activator; D017228:Hepatocyte Growth Factor; C028190:5-oxo-prolyl-glycyl-arginine-4-nitroanilide; D019859:Proto-Oncogene Proteins c-met; D014568:Urokinase-Type Plasminogen Activator