Synthesis and deprotection of oligonucleotides under aprotic conditions.
Oligonucleotides containing an alkali-labile nucleotide are synthesized and deprotected using a synthetic method that eliminates the use of Bronsted acid and base. The development of a new family of exocyclic amine-protecting groups is an integral component of this approach.
Chen
T
T
Department of Chemistry, Colorado State University, Ft. Collins, Colorado 80523, USA.
Fu
J
J
Greenberg
M M
MM
D007202:Indicators and Reagents; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
D007202:Indicators and Reagents; D009841:Oligonucleotides