title: Remarkable proteolytic activity of imidazoles attached to cross-linked polystyrene.
abstract: The insoluble resins synthesized by attaching imidazoles to poly(chloromethylstyrene-co-divinylbenzene) effectively hydrolyzed albumin with half-life as short as 20 min at pH 7 and 25 degrees C. Thus, peptide hydrolysis was accomplished with imidazole in an artificial system for the first time. The imidazole-based artificial proteinases manifested optimum activity at pH 7-8. The proteolytic activity of the imidazole-based artificial proteinases exceeded that of previously reported organic artificial proteinases including catalytic antibodies. High proteolytic activity was observed when imidazole was attached to the resin through the C-2 atom instead of the N atom. The catalytic activity was greatly reduced when the content of imidazole was lowered. This indicates catalytic cooperation of at least two proximal imidazole moieties attached to the resin. Possible mechanisms for the effective protein hydrolysis by the proximal imidazoles are presented.
authors.affiliation: School of Chemistry and Molecular Engineering and Center for Molecular Catalysis, Seoul National University, Seoul 151-742, Korea. jhsuh@snu.ac.kr
authors.forename: J
authors.initials: J
authors.lastname: Suh
authors.forename: S
authors.initials: S
authors.lastname: Oh
mesh_terms: D003432:Cross-Linking Reagents; D010450:Endopeptidases / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000737:chemistry*; D011137:Polystyrenes
chemical_list: D003432:Cross-Linking Reagents; D007093:Imidazoles; D011137:Polystyrenes; D010450:Endopeptidases