Establishment of IPC 227 cells as human xenografts in rabbits: a model of uveal melanoma.
This study was designed in order to evaluate the feasibility of establishing an animal model of human uveal melanoma. IPC227, a cell line established from the biopsy of a patient with a spindle cell ciliary body melanoma, was transplanted into the anterior chamber of the eye in immunosuppressed New Zealand rabbits. In a second step, a tumour fragment from the anterior chamber was implanted transclerally into the posterior choroid. Complete ophthalmological examinations were then performed on the animals. Characteristic growth patterns were noted depending on the location of implantation. In the anterior chamber, diffuse, flat, heavily pigmented tumours appeared 8 days after the injection of the cell suspension that covered the iris and the angle by day 25, with a success rate of 100%. Nodular, lightly pigmented tumours were obtained 6-7 weeks after subchoroidal implantation, with a 25% success rate. Clinical examination, including fundus photography, ultrasound and magnetic resonance imaging, demonstrated the same characteristics as those of human uveal melanoma, confirming the value of this model for the evaluation of new therapeutic and diagnostic methods in the management of uveal melanoma.
Bonicel
P
P
CHU Gabriel Montpied, Service d'Ophtalmologie, Clermont-Ferrand, France. pbonicel@chu-clermontferrand.fr
Michelot
J
J
Bacin
F
F
Papon
J
J
Kemeny
J L
JL
Moins
N
N
Morvan
D
D
Madelmont
J C
JC
D000818:Animals; D001706:Biopsy; D018929:Cell Culture Techniques / Q000379:methods; D002830:Choroid Neoplasms / Q000473:pathology*; D016572:Cyclosporine / Q000627:therapeutic use; D005260:Female; D006801:Humans; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D008545:Melanoma / Q000473:pathology*; D009368:Neoplasm Transplantation; D011817:Rabbits; D014183:Transplantation, Heterologous / Q000276:immunology / Q000473:pathology; D014407:Tumor Cells, Cultured; D014604:Uveal Neoplasms / Q000473:pathology*
D007166:Immunosuppressive Agents; D016572:Cyclosporine