Effects of gastric inhibitory polypeptide, somatostatin and epidermal growth factor on lipogenesis in ovine adipose explants.
Feeding raises the plasma concentrations of a number of gut-related hormones that may, in turn, influence the metabolism of peripheral tissues. This study investigated the effects of gut-related hormones on lipogenesis in explants from three differing adipose depots in lambs (aged 4-9 months). Incorporation of [14C]-acetate into lipid was measured over a 2-h period, following 24 h pre-incubation in the presence of hormone combinations. In perirenal fat explants, gastric inhibitory polypeptide (GIP) in the concentration range 0.01-10 nM stimulated lipogenesis. Maximal effects were seen at 1 nM (an average increase of 64% over basal values). In contrast, in the presence of insulin (0.1 nM), a dose-dependent decrease in lipogenesis was seen with increasing GIP concentration (P < 0.001 for the insulin x GIP interaction). Epidermal growth factor (EGF) and somatostatin in the same concentration range each inhibited lipogenesis. both in the presence and the absence of insulin (P < 0.001 in each case). Subcutaneous (back) fat and intermuscular (popliteal) fat responded similarly to each other, but significantly differently from the perirenal depot (P < 0.001). Here GIP, somatostatin or EGF (each at 1 nM) all separately stimulated lipogenesis.
Baba
A S
AS
Division of Nutritional Biochemistry, School of Biosciences, University of Nottingham, Loughhorough, Leicestershire, UK. ahmad@botany.um.edu.my
Harper
J M
JM
Buttery
P J
PJ
D000273:Adipose Tissue / Q000187:drug effects* / Q000502:physiology*; D000818:Animals; D046508:Culture Techniques; D004305:Dose-Response Relationship, Drug; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005749:Gastric Inhibitory Polypeptide / Q000494:pharmacology*; D012756:Sheep; D013004:Somatostatin / Q000494:pharmacology*; D013997:Time Factors
D013004:Somatostatin; D005749:Gastric Inhibitory Polypeptide; D004815:Epidermal Growth Factor