title: Identification of c-myc responsive genes using rat cDNA microarray.
abstract: c-Myc functions through direct activation or repression of transcription. Using cDNA microarray analysis, we have identified c-Myc-responsive genes by comparing gene expression profiles between c-myc null and c-myc wild-type rat fibroblast cells and between c-myc null and c-myc null cells reconstituted with c-myc. From a panel of 4400 cDNA elements, we found 198 genes responsive to c-myc when comparing wild-type or reconstituted cells with the null cells. The plurality of the named c-Myc-responsive genes that were up-regulated, including 30 ribosomal protein genes, are involved in macromolecular synthesis and metabolism, suggesting a major role of c-Myc in the regulation of protein synthetic and metabolic pathways. When ectopically overexpressed, c-Myc induced a different and smaller set of c-Myc-responsive genes as compared with the physiologically expressed c-Myc condition. Thus, these results from expression profiling suggest a new primary function for c-Myc and raise the possibility that the physiological and transforming functions of c-myc may be separable.
authors.affiliation: Molecular Signaling and Oncogenesis Section, Department of Cancer and Cell Biology, Medicine Branch, Division of Clinical Science, National Cancer Institute, Bethesda, Maryland 20892, USA.
authors.forename: Q M
authors.initials: QM
authors.lastname: Guo
authors.forename: R L
authors.initials: RL
authors.lastname: Malek
authors.forename: S
authors.initials: S
authors.lastname: Kim
authors.forename: C
authors.initials: C
authors.lastname: Chiao
authors.forename: M
authors.initials: M
authors.lastname: He
authors.forename: M
authors.initials: M
authors.lastname: Ruffy
authors.forename: K
authors.initials: K
authors.lastname: Sanka
authors.forename: N H
authors.initials: NH
authors.lastname: Lee
authors.forename: C V
authors.initials: CV
authors.lastname: Dang
authors.forename: E T
authors.initials: ET
authors.lastname: Liu
mesh_terms: D000818:Animals; D002460:Cell Line; D018076:DNA, Complementary / Q000235:genetics; D015536:Down-Regulation; D005347:Fibroblasts / Q000502:physiology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology; D016259:Genes, myc / Q000502:physiology*; D020411:Oligonucleotide Array Sequence Analysis; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D051381:Rats; D015854:Up-Regulation
chemical_list: D018076:DNA, Complementary; D011506:Proteins; D016271:Proto-Oncogene Proteins c-myc