Atrazine degradation in a containerized rhizosphere system.
The effect of atrazine (2-chloro-4-ethylamino-6-isopropylamino-s-triazine) on rhizosphere microorganisms and its fate in a containerized rhizosphere system was studied. The rhizosphere system consisted of corn grown in pot containing a defined potting mix of sand and bark with atrazine. Sterilized potting mix and a container without plants served as controls. Atrazine was extracted and analyzed via HPLC. Fluorescent pseudomonad populations increased 100-fold in the rhizposphere during a 60-day incubation period as compared to the nonvegetated control. Atrazine degradation was higher in the rhizosphere system (half-life of 7 days) compared to the nonvegetated control (half-life of greater than 45 days). The major degradation product detected in the rhizosphere system was deisopropylatrazine; other products detected included deethylatrazine, deethylhydroxyatrazine, deisopropylatrazine and hydroxyatrazine. Hydroxyatrazine was detected in the nonvegetated and sterile controls. The containerized rhizosphere system provides an experimental system to study the fate of pesticidal chemicals as well as the effects on microbial populations.
Costa
R M
RM
Department of Microbiology and Molecular Medicine, Clemson University, SC 29634-0377, USA.
Camper
N D
ND
Riley
M B
MB
D001280:Atrazine / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D006207:Half-Life; D006540:Herbicides / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D003313:Zea mays / Q000187:drug effects* / Q000382:microbiology
D006540:Herbicides; D001280:Atrazine