Short-term exposure to membrane-active antibiotics inhibits Cryptosporidium parvum infection in cell culture.
A cell culture system and double fluorogenic staining were used to study the susceptibility of Cryptosporidium parvum to membrane-active antibiotics. Buforin II and magainin II exerted a cytotoxic effect on sporozoites but did not consistently affect oocyst viability. Lasalocid and nigericin demonstrated less activity against sporozoites but reduced the infectivity of oocysts.
Giacometti
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A
Institute of Infectious Diseases and Public Health, University of Ancona, Ancona, Italy. cmalinf@popsci.unian.it
Cirioni
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Del Prete
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MS
Barchiesi
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Scalise
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G
D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects; D016785:Cryptosporidium parvum / Q000166:cytology / Q000187:drug effects*; D004353:Drug Evaluation, Preclinical; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D006801:Humans; D054806:Magainins; D011506:Proteins / Q000494:pharmacology*; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000469:parasitology; D029867:Xenopus Proteins
D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D005456:Fluorescent Dyes; D054806:Magainins; D011506:Proteins; D029867:Xenopus Proteins; C097849:buforin II; C053301:magainin 2 peptide, Xenopus