Inhibition of human epithelial ovarian cancer cell growth in vitro by somatostatin analog RC-160.
In this study, we investigated the effects of somatostatin analog RC-160 on the growth of the OV-1063 human epithelial ovarian cancer cell line in vitro. RC-160 inhibited cell proliferation, as measured by cell number, and [(3)H]thymidine incorporation into DNA at 10(-9)-10(-5) M. In OV-1063 cells, (125)I-labeled RC-160 was bound to one class of specific, saturable binding sites with high affinity (K(d) = 0.2 +/- 0.03 nM) and low capacity (5,500 binding sites per cell). (125)I-labeled RC-160 could be displaced by unlabeled RC-160. Ligand binding was dependent on time and temperature. Receptor internalization assay showed that the ligand-receptor complex was internalized at 37 degrees C, which indicates the presence of biologically active somatostatin receptors on OV-1063 cells. These results suggest that somatostatin analog RC-160 can suppress the growth of OV-1063 human epithelial ovarian cancer cells by a direct action and that the inhibitory effect of somatostatin analog is mediated through the high-affinity somatostatin receptors.
Yano
T
T
Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Hongua, Japan. tetu-tky@umin.ac.jp
Radulovic
S
S
Osuga
Y
Y
Kugu
K
K
Yoshikawa
H
H
Taketani
Y
Y
Schally
A V
AV
D000970:Antineoplastic Agents / Q000378:metabolism / Q000494:pharmacology*; D002277:Carcinoma / Q000188:drug therapy* / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D005260:Female; D006801:Humans; D007457:Iodine Radioisotopes; D010051:Ovarian Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D017481:Receptors, Somatostatin / Q000187:drug effects; D013004:Somatostatin / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D007457:Iodine Radioisotopes; D017481:Receptors, Somatostatin; C051458:vapreotide; D013004:Somatostatin