Screening of expression libraries using ELISA: identification of immunogenic proteins from Tityus bahiensis and Tityus serrulatus venom.
The present report describes the use of ELISA with cDNA expression libraries in the identification of immunogenic proteins. The methodology described was applied using libraries constructed with mRNA isolated from Tityus serrulatus and Tityus bahiensis venom glands. In addition we describe for the first time the sequence of a neurotoxin from Tityus bahiensis venom gland named TbTx5 whose amino acid sequencing showed 93% similarity with the Tityus bahiensis TbTx IV-5 neurotoxin. The methodology described can be used for the generation of an immunogenic bank in order to contribute to genome and proteome projects.
Kalapothakis
E
E
Divisão de Biologia Celular, Departamento de Farmacologia, ICB, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, MG 31-270901, Belo Horizonte, Brazil. ekalapo@mono.icb.ufmg.br
Jardim
S
S
Magalhães
A C
AC
Mendes
T M
TM
De Marco
L
L
Afonso
L C
LC
Chávez-Olórtegui
C
C
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D015723:Gene Library; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000302:isolation & purification; D011817:Rabbits; D012604:Scorpion Venoms / Q000235:genetics* / Q000302:isolation & purification / Q000633:toxicity; D017386:Sequence Homology, Amino Acid
D012333:RNA, Messenger; D012604:Scorpion Venoms