Isolation and identification of adenovirus in hospitalized children, under five years, with acute respiratory disease, in Havana, Cuba.
Nine Adenovirus (Ad) strains isolated in Cuba, from 128 nasopharingeal swab specimens of children below five years old, with acute respiratory diseases, during 1996 and 1997, were studied by restriction enzyme analysis of genomic DNA with two endonucleases BamH I and Sma I. All different fragment patterns were compared with the respective prototypes. The identified adenoviruses were Ad 1 (n=4), Ad 2 (n=1) and Ad 6 (n=4). Males were more frequently infected than females. The analysis of the occurrence of these Adenovirus strains of subgenus C revealed that Ad 1 and Ad 6 were the predominant serotypes in 1996 and in 1997, respectively.
Pumariega
T
T
Instituto de Medicina Tropical Pedro Kourí, Habana, Cuba.
Savón
C
C
Muné
M
M
Cancio
R
R
González
G
G
Valdivia
A
A
González
Z
Z
Goyenechea
A
A
D000208:Acute Disease; D000258:Adenovirus Infections, Human / Q000175:diagnosis; D000260:Adenoviruses, Human / Q000302:isolation & purification*; D002675:Child, Preschool; D003462:Cuba; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D012141:Respiratory Tract Infections / Q000175:diagnosis / Q000821:virology*
D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI