title: Myosin II regulatory light chain as a novel substrate for AIM-1, an aurora/Ipl1p-related kinase from rat.
abstract: Previous studies demonstrated that the phosphorylated myosin II regulatory light chain (MRLC) is localized at the cleavage furrow of dividing cells, suggesting that phosphorylation of MRLC plays an important role in cytokinesis. However, it remains unclear which kinase(s) phosphorylate MRLC during cytokinesis. AIM-1, an Aurora/Ipl1p-related kinase from rat, is known as a serine/threonine kinase that is required for cytokinesis. Here we examined the possibility that AIM-1 is a candidate for a kinase that phosphorylates MRLC during cytokinesis. As a result, we showed that AIM-1 monophosphorylated MRLC at Ser19 using two-dimensional phosphopeptide mapping analysis and several MRLC mutants. Furthermore, AIM-1 was colocalized with monophosphorylated MRLC at the cleavage furrow of dividing cells. We propose here that AIM-1 may participate in monophosphorylation of MRLC during cytokinesis.
authors.affiliation: Department of Biological Science, Graduate School of Science, Hiroshima University, Higashi-Hiroshima 739-8526, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Murata-Hori
authors.forename: K
authors.initials: K
authors.lastname: Fumoto
authors.forename: Y
authors.initials: Y
authors.lastname: Fukuta
authors.forename: T
authors.initials: T
authors.lastname: Iwasaki
authors.forename: A
authors.initials: A
authors.lastname: Kikuchi
authors.forename: M
authors.initials: M
authors.lastname: Tatsuka
authors.forename: H
authors.initials: H
authors.lastname: Hosoya
mesh_terms: D000818:Animals; D064127:Aurora Kinases; D002460:Cell Line; D006801:Humans; D009218:Myosins / Q000737:chemistry / Q000378:metabolism*; D010449:Peptide Mapping; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases; D051381:Rats; D013379:Substrate Specificity
chemical_list: D011494:Protein Kinases; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases; D009218:Myosins