title: Anaerobic toluene activation by benzylsuccinate synthase in a highly enriched methanogenic culture.
abstract: Permeabilized cells of a highly enriched, toluene-mineralizing, methanogenic culture catalyzed the addition of toluene to fumarate to form benzylsuccinate under anaerobic conditions. The specific in vitro rate of benzylsuccinate formation was >85% of the specific in vivo rate of toluene consumption. This is the first report of benzylsuccinate synthase activity in a methanogenic culture; the activity has previously been reported to occur in denitrifying, sulfate-reducing, and anoxygenic phototrophic bacteria.
authors.affiliation: Lawrence Livermore National Laboratory, Livermore, California 94551-0808, USA. beller2@llnl.gov
authors.forename: H R
authors.initials: HR
authors.lastname: Beller
authors.forename: E A
authors.initials: EA
authors.lastname: Edwards
mesh_terms: D000693:Anaerobiosis; D001673:Biodegradation, Environmental; D019755:Carbon-Carbon Lyases / Q000378:metabolism*; D019605:Euryarchaeota / Q000201:enzymology* / Q000378:metabolism*; D007700:Kinetics; D014050:Toluene / Q000378:metabolism*
chemical_list: D014050:Toluene; D019755:Carbon-Carbon Lyases; C114354:benzylsuccinate synthase