Delivery of Neospora caninum surface protein, NcSRS2 (Nc-p43), to mouse using recombinant vaccinia virus.
In order to develop a vaccine against Neospora caninum in dogs and cattle, we constructed a recombinant vaccinia virus expressing the N. caninum surface protein, NcSRS2 (Nc-p43). Monoclonal antibodies to NcSRS2 and anti-N. caninum tachyzoite mouse serum recognized the NcSRS2 expressed by the recombinant vaccinia virus. In addition, recombinant NcSRS2 was transported to the cell surface. Mice infected with the recombinant virus predominantly produced IgG1 antibody (Ab) to N. caninum, rather than producing IgG2a Ab. Moreover, splenocytes from mice infected with the recombinant virus proliferated in the presence of the N. caninum antigen. Mice immunized with the recombinant virus gave rise to humoral and cellular immune responses to N. caninum tachyzoites. This study showed that a recombinant vaccinia virus expressing NcSRS2 might be useful for the production of a live vaccine against N. caninum infection.
Nishikawa
Y
Y
The National Research Center for Protozoan Diseases, Obihiro University, Hokkaido, Japan.
Kousaka
Y
Y
Fukumoto
S
S
Xuan
X
X
Nagasawa
H
H
Igarashi
I
I
Fujisaki
K
K
Otsuka
H
H
Mikami
T
T
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D015153:Blotting, Western; D002460:Cell Line; D003048:Coccidiosis / Q000276:immunology* / Q000469:parasitology / Q000517:prevention & control; D005260:Female; D005822:Genetic Vectors; D007114:Immunization; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D018688:Neospora / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D015800:Protozoan Proteins / Q000235:genetics* / Q000378:metabolism; D016052:Protozoan Vaccines / Q000235:genetics / Q000276:immunology*; D011995:Recombination, Genetic; D013154:Spleen / Q000276:immunology; D014616:Vaccinia virus / Q000235:genetics* / Q000378:metabolism
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; C115389:SRS2 protein, Neospora caninum