title: Percutaneous absorption of sunscreens in vitro: interspecies comparison, skin models and reproducibility aspects.
abstract: Appropriate evaluation of sunscreens is required to provide better knowledge of their safety and efficacy. One of the most important elements of this evaluation is the assessment of percutaneous absorption. In vitro methods are largely used for such assessments, and the accuracy of the measurements generated with these methods depends on the use of a proper methodology. This study was designed to evaluate an in vitro protocol for investigating the percutaneous absorption of two sunscreens under standardized experimental conditions. Octyl methoxycinnamate and benzophenone 4 were each incorporated in a typical oil-in-water emulsion and tested separately. Salicylic acid was tested as a reference compound. In vitro percutaneous absorption was evaluated using two species, the pig and human, and two models, full-thickness and split-thickness skin. The reproducibility of study results was evaluated by comparing the data generated by two industrial laboratories, L'Oréal and Hoffmann-La Roche. The correlation of quantitative data between pig skin and human skin was very good, and the split-thickness skin model seemed to be more appropriate for measuring the absorption of sunscreens. Results obtained for salicylic acid demonstrated the relevance of the protocol in terms of prediction of in vivo percutaneous absorption. Finally, the comparison of pig skin data between the two laboratories demonstrated a good correlation and underlined the need for a standardized in vitro procedure.
authors.affiliation: Life Sciences, Advanced Research Laboratories, L'Oréal, Aulnay-sous-Bois, France.
authors.forename: F
authors.initials: F
authors.lastname: Benech-Kieffer
authors.forename: P
authors.initials: P
authors.lastname: Wegrich
authors.forename: R
authors.initials: R
authors.lastname: Schwarzenbach
authors.forename: G
authors.initials: G
authors.lastname: Klecak
authors.forename: T
authors.initials: T
authors.lastname: Weber
authors.forename: J
authors.initials: J
authors.lastname: Leclaire
authors.forename: H
authors.initials: H
authors.lastname: Schaefer
mesh_terms: D000818:Animals; D001577:Benzophenones / Q000493:pharmacokinetics; D002934:Cinnamates / Q000493:pharmacokinetics; D004058:Diffusion; D006801:Humans; D066298:In Vitro Techniques; D007553:Isotope Labeling; D008566:Membranes / Q000378:metabolism; D015203:Reproducibility of Results; D020156:Salicylic Acid / Q000493:pharmacokinetics; D012869:Skin Absorption; D013045:Species Specificity; D013473:Sunscreening Agents / Q000493:pharmacokinetics*; D013552:Swine
chemical_list: D001577:Benzophenones; D002934:Cinnamates; D013473:Sunscreening Agents; C118580:octylmethoxycinnamate; D020156:Salicylic Acid