Expression of cbsA encoding the collagen-binding S-protein of Lactobacillus crispatus JCM5810 in Lactobacillus casei ATCC 393(T).
The cbsA gene encoding the collagen-binding S-layer protein of Lactobacillus crispatus JCM5810 was expressed in L. casei ATCC 393(T). The S-protein was not retained on the surface of the recombinant bacteria but was secreted into the medium. By translational fusion of CbsA to the cell wall sorting signal of the proteinase, PrtP, of L. casei, CbsA was presented at the surface, rendering the transformants able to bind to immobilized collagens.
Martínez
B
B
Department of Applied Microbiology and Gene Technology, TNO Voeding, 3700 AJ Zeist, The Netherlands.
Sillanpää
J
J
Smit
E
E
Korhonen
T K
TK
Pouwels
P H
PH
D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D002462:Cell Membrane / Q000378:metabolism; D016023:Integrins / Q000096:biosynthesis* / Q000235:genetics; D007778:Lactobacillus / Q000235:genetics*; D007780:Lacticaseibacillus casei / Q000235:genetics / Q000378:metabolism*; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D038981:Receptors, Collagen
D001426:Bacterial Proteins; D016023:Integrins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D038981:Receptors, Collagen; C055764:S-layer proteins