Regional specification during embryogenesis in the craniiform brachiopod Crania anomala.
A fate map has been constructed for the embryo of Crania. The animal half of the egg forms the ectodermal epithelium of the larva's apical lobe. The vegetal half of the egg forms endoderm, mesoderm, and the ectoderm of the mantle lobe. The vegetal pole is the site of gastrulation; this site becomes the posterior ventral region of the mantle lobe of the larva. The plane of the first cleavage goes through the animal-vegetal axis of the egg; it bears no relationship to the future plane of bilateral symmetry of the larva. The timing of regional specification was examined by isolating animal, vegetal, or meridional halves from oocytes, eggs, or embryos from prior to germinal vesicle breakdown through gastrulation. Animal halves isolated from oocytes formed either the epithelium of the apical lobe or a larva with all three germ layers. Animal halves isolated from unfertilized eggs and eight-cell embryos formed only apical lobe epithelium. Beginning at the blastula stage, animal halves formed mantle in addition to apical lobe epithelium. In animal halves isolated after gastrulation, the mantle lobe was always truncated. Vegetal halves isolated at all stages prior to gastrulation gastrulated and formed apical and mantle lobes with endoderm and mesoderm; however, the relative size of the apical lobe that formed decreased substantially when vegetal halves were isolated at later developmental stages. When meridional halves were isolated from unfertilized eggs and two- to four-cell embryos, both halves frequently formed normally proportioned larvae. Beginning at the blastula stage, a number of pairs frequently had a member that lacked dorsal setae on its mantle lobe while the other member of the pair formed setae, indicating that the dorsoventral axis had been set up. The process of regional specification in Crania is compared to those of Discinisca and Glottidia in the brachiopod subphylum Linguliformea and Phoronis in the phylum Phoronida.
Freeman
G
G
Kristineberg Marine Research Station, Fiskebackskil, Sweden.
D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism; D019521:Body Patterning; D019070:Cell Lineage; D048429:Cell Size; D004475:Ectoderm / Q000378:metabolism; D004707:Endoderm / Q000378:metabolism; D005260:Female; D005307:Fertilization in Vitro; D005775:Gastrula / Q000166:cytology / Q000378:metabolism; D006651:Histocytochemistry; D007448:Invertebrates / Q000166:cytology* / Q000196:embryology*; D007814:Larva / Q000166:cytology / Q000378:metabolism; D008648:Mesoderm / Q000378:metabolism; D009865:Oocytes / Q000166:cytology / Q000254:growth & development; D009866:Oogenesis / Q000502:physiology; D006080:Ovarian Follicle / Q000166:cytology / Q000502:physiology; D010802:Phylogeny