A specific wheat germ agglutinin-immunoreactive protein in human placenta.
In this study we examined human placenta for the presence of molecules antigenically related to a plant lectin, wheat germ agglutinin. The initial results of immunolocalization using polyclonal antibodies against wheat germ agglutinin showed that human placenta contains protein(s) recognized specifically. Staining of syncytiotrophoblast brush border and cytotrophoblast, granular in appearance was observed in first trimester human placenta. Specific binding was also seen in trophoblast-derived JAr and BeWo carcinoma cells. Isolation of wheat germ agglutinin-immunoreactive material from human placenta was achieved by ion-exchange- and affinity-chromatography on anti-wheat germ agglutinin-immunoglobulin G-Sepharose. The placental protein having molecular mass of 66 kD was identified as specific. The protein of 66 kD was characterized as a calcium-dependent, asialofetuin-binding molecule.
Janković
M
M
Institute for the Application of Nuclear Energy - INEP, Zemun, Yugloslavia. miraj@inep.co.yu
Vićovac
L
L
D000906:Antibodies / Q000276:immunology; D001212:Asialoglycoproteins / Q000378:metabolism; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism; D002846:Chromatography, Affinity; D002847:Chromatography, Agarose; D002852:Chromatography, Ion Exchange; D004306:Dose-Response Relationship, Immunologic; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D060748:Fetuins; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008024:Ligands; D010920:Placenta / Q000737:chemistry*; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D011485:Protein Binding; D014327:Trophoblasts / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014909:Wheat Germ Agglutinins / Q000737:chemistry* / Q000276:immunology* / Q000302:isolation & purification; D000509:alpha-Fetoproteins / Q000378:metabolism
D000906:Antibodies; D001212:Asialoglycoproteins; D060748:Fetuins; D008024:Ligands; D014909:Wheat Germ Agglutinins; D000509:alpha-Fetoproteins; C017029:asialofetuin; D002118:Calcium