Effect of Baypamun on blood leucocytes in normal and dexamathasone treated goats.
The aim of this study was to determine the effect of Baypamun on selected lymphocyte subpopulations and granulocyte phagocytic activity mediated by lectin-like receptors in goats of normal immune status and in goats experimentally immunosuppressed with dexamethasone. Eighteen goats in total were used. Blood samples were collected 24 h before immunomodulation and 5 and 10 days after immunomodulation. Animals in group I were immunostimulated with Baypamun for 2 days and immunosuppressed with Dexafort for the next 2 days. Animals in group II received Dexafort first and then Baypamun. The number of leucocytes in total and in subpopulations was determined by flow cytometry. Application of Baypamun before (group I) or after (group II) immunosuppression caused significant (P < 0.001) and lasting changes in the percentage of CD2+, CD4+ and CD8+ T cells. Significant but transient changes were observed in CD19+ (B) and WC1-N2+ (null) cells. Results show that application of Baypamun to modulate non-specific defence is advisable following immunosuppression (group I). When Baypamun was applied after immunosuppression (group II), although there was no reaction during the initial phase, an increase in the activity of cells responsible for non-specific immunity was noticeable after 10 days.
Winnicka
A
A
Agricultural University of Warsaw, Poland.
Klucinski
W
W
Kawiak
J
J
Hoser
G
G
Sikora
J
J
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000911:Antibodies, Monoclonal; D003907:Dexamethasone / Q000494:pharmacology*; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D005938:Glucocorticoids / Q000494:pharmacology*; D006041:Goats / Q000097:blood* / Q000276:immunology; D007150:Immunohistochemistry; D007165:Immunosuppression Therapy; D007202:Indicators and Reagents / Q000737:chemistry; D007962:Leukocytes / Q000187:drug effects* / Q000276:immunology; D009580:Nitroblue Tetrazolium / Q000737:chemistry; D010587:Phagocytosis / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D018709:Statistics, Nonparametric; D014765:Viral Vaccines / Q000494:pharmacology*
D000276:Adjuvants, Immunologic; D000911:Antibodies, Monoclonal; C076692:Baypamun HK; D005938:Glucocorticoids; D007202:Indicators and Reagents; D014765:Viral Vaccines; D009580:Nitroblue Tetrazolium; D003907:Dexamethasone