Urinary metabolites of gallic acid in rats and their radical-scavenging effects on 1,1-diphenyl-2-picrylhydrazyl radical.
As a part of our studies on the metabolism of natural compounds, gallic acid was orally administered to rats. The urinary metabolites were analyzed by high-performance liquid chromatography, and their structures were determined to be pyrogallol (M1), pyrogallol-1-O-beta-D-glucuronide (M2), 4-O-methylgallic acid-3-O-sulfate (M3), 2-O-methylpyrogallol-1-O-beta-D-glucuronide (M4), 2-O-methylpyrogallol (M5), 4-O-methylgallic acid (M6), and unchanged gallic acid on the basis of chemical and spectral data. The radical scavenging effects of gallic acid and its urinary metabolites were evaluated using 1,1-diphenyl-2-picrylhydrazyl radical.
Yasuda
T
T
Department of Phytochemistry, Tohoku Pharmaceutical University, 4-1 Komatsushima 4 Chome, Aoba-ku, Sendai, Japan.
Inaba
A
A
Ohmori
M
M
Endo
T
T
Kubo
S
S
Ohsawa
K
K
D000818:Animals; D015764:Bepridil / Q000031:analogs & derivatives* / Q000378:metabolism*; D001713:Biphenyl Compounds; D002851:Chromatography, High Pressure Liquid; D016166:Free Radical Scavengers / Q000378:metabolism / Q000652:urine; D005707:Gallic Acid / Q000378:metabolism / Q000652:urine*; D008297:Male; D015394:Molecular Structure; D010851:Picrates; D051381:Rats
D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D005707:Gallic Acid; D015764:Bepridil; C004931:1,1-diphenyl-2-picrylhydrazyl