title: [Overall genomic DNA methylation in relation to estrogen].
abstract: Overall genomic DNA methylation was analyzed using enzymatic digestion into nucleotides, 32P postlabeling, two-dimensional thin-layer chromatography on cellulose plates and phosphobioimaging quantitation, in relation to immunohistochemically measured estrogen (ER) and progesterone receptor (PR) status of 15 uterine cancers. Mean 5-methyldeoxycytosine (m5dC) content did not differ between ER-positive and ER-negative neoplasms. Highest values of m5dC were noted both in ER-negative and ER-positive tumors. Additionally, there was no low DNA methylation in ER negative uterine cancer tissues. Decrease of the overall genomic DNA methylation could be related to the increase of ER/PR ratio, however it was not significant in our investigation. The potential role of steroid receptors status in uterine cancer tissue is discussed.
authors.affiliation: Kliniki Ginekologii Operacyjnej II Katedry Połoznictwa i Chorób Kobiecych AM w Lublinie.
authors.forename: K
authors.initials: K
authors.lastname: Postawski
authors.forename: E
authors.initials: E
authors.lastname: Olech-Fudali
authors.forename: J A
authors.initials: JA
authors.lastname: Jakowicki
authors.forename: E
authors.initials: E
authors.lastname: Korobowicz
authors.forename: G
authors.initials: G
authors.lastname: Keith
authors.forename: W
authors.initials: W
authors.lastname: Baranowski
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D019175:DNA Methylation; D015254:DNA Modification Methylases / Q000378:metabolism; D005260:Female; D006801:Humans; D008875:Middle Aged; D011960:Receptors, Estrogen / Q000378:metabolism*; D011980:Receptors, Progesterone / Q000378:metabolism*; D014594:Uterine Neoplasms / Q000378:metabolism* / Q000473:pathology*; D014599:Uterus / Q000378:metabolism* / Q000473:pathology
chemical_list: D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D015254:DNA Modification Methylases