title: Characterization of amiodarone metabolites and impurities using liquid chromatography/atmospheric pressure chemical ionization mass spectrometry.
abstract: Using the high performance liquid chromatography/atmospheric pressure chemical ionization tandem mass spectrometry (HPLC/APCI-MS/MS) technique, together with established trends from the literature, the structures of metabolites and impurities of amiodarone, an anti-arrhythmic drug, have been assigned. By comparing analyses of products of incubation with rat liver microsomes with controls in which glucose 6-phosphate dehydrogenase was omitted, metabolites could be distinguished from impurities. Structures for the two proposed metabolites and four impurities are proposed.
authors.affiliation: Doping Control Center, Korea Institute of Science and Technology, Seoul. swmyung@kist.re.kr
authors.forename: S W
authors.initials: SW
authors.lastname: Myung
authors.forename: Y J
authors.initials: YJ
authors.lastname: Chang
authors.forename: H K
authors.initials: HK
authors.lastname: Min
authors.forename: D H
authors.initials: DH
authors.lastname: Kim
authors.forename: M
authors.initials: M
authors.lastname: Kim
authors.forename: T K
authors.initials: TK
authors.lastname: Kang
authors.forename: E A
authors.initials: EA
authors.lastname: Yoo
authors.forename: Y T
authors.initials: YT
authors.lastname: Sohn
authors.forename: Y H
authors.initials: YH
authors.lastname: Yim
mesh_terms: D000638:Amiodarone / Q000031:analogs & derivatives / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D001274:Atmospheric Pressure; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D004048:Diethylamines / Q000032:analysis / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods*; D008862:Microsomes, Liver / Q000378:metabolism; D015394:Molecular Structure; D051381:Rats
chemical_list: D000889:Anti-Arrhythmia Agents; D004048:Diethylamines; C034281:diethylamine; D005954:Glucosephosphate Dehydrogenase; C036116:desethylamiodarone; D000638:Amiodarone