Working at the cutting edge: the creation of allosteric ribozymes.
The appropriate folding of catalytic RNA is a prerequisite for effective catalysis. A novel ribozyme, the maxizyme, has been generated and its activity can be controlled allosterically. The maxizymes work both in vitro and in vivo indicating the potential utility of this novel class of ribozyme as a gene-inactivating agent with a biosensor function.
Warashina
M
M
National Institute for Advanced Interdisciplinary Research AIST, MITI 305-8562, Tsukuba Science City, Japan.
Kuwabara
T
T
Taira
K
K
D000255:Adenosine Triphosphate / Q000502:physiology; D000494:Allosteric Regulation; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D001665:Binding Sites; D002384:Catalysis; D004247:DNA / Q000502:physiology; D019281:Dimerization; D005486:Flavin Mononucleotide / Q000502:physiology; D016044:Fusion Proteins, bcr-abl / Q000235:genetics; D016313:Genes, abl; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics / Q000473:pathology; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D008958:Models, Molecular; D009368:Neoplasm Transplantation; D009690:Nucleic Acid Conformation; D010957:Plasmids / Q000235:genetics; D012313:RNA / Q000502:physiology; D016337:RNA, Catalytic / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D012343:RNA, Transfer / Q000737:chemistry; D017384:Sequence Deletion; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D014407:Tumor Cells, Cultured / Q000187:drug effects; D023041:Xenograft Model Antitumor Assays
D000970:Antineoplastic Agents; D016337:RNA, Catalytic; D012313:RNA; D005486:Flavin Mononucleotide; D000255:Adenosine Triphosphate; D004247:DNA; D012343:RNA, Transfer; D016044:Fusion Proteins, bcr-abl