Transgenic apple (Malus x domestica) shoot showing low browning potential.
Transgenic apple shoots were prepared from leaf disks by using Agrobacterium tumefaciens carrying the kanamycin (KM) resistance gene and antisense polyphenol oxidase (PPO) DNA. Four transgenic apple lines that grew on the medium containing 50 microgram/mL KM were obtained. They contained the KM resistance gene and grew stably on the medium for >3 years. Two transgenic shoot lines containing antisense PPO DNA in which PPO activity was repressed showed a lower browning potential than a control shoot.
Murata
M
M
Department of Nutrition and Food Science, Ochanomizu University, 2-1-1 Otsuka, Bunkyo-ku, Tokyo 112-8610, Japan. murata@cc.ocha.ac.jp
Haruta
M
M
Murai
N
N
Tanikawa
N
N
Nishimura
M
M
Homma
S
S
Itoh
Y
Y
D016960:Agrobacterium tumefaciens / Q000235:genetics; D004156:Catechol Oxidase / Q000235:genetics; D016373:DNA, Antisense; D005638:Fruit / Q000201:enzymology / Q000235:genetics* / Q000502:physiology; D005822:Genetic Vectors; D007613:Kanamycin Resistance / Q000235:genetics; D018520:Plant Shoots; D030821:Plants, Genetically Modified
D016373:DNA, Antisense; D004156:Catechol Oxidase