title: Masculine copulatory behavior is facilitated by intrathecally administered muscarine.
abstract: Behavioral experiments were conducted to examine the role of the cholinergic receptor-agonist muscarine or its antagonist homatropine on the mating behavior of sexually experienced male rats. Male copulatory behavior was recorded after intrathecally administered saline, muscarine (7.5 microg), or homatropine (25 microg). Changes in copulatory behavior were assessed by the following parameters: intromission latency, intromission frequency, intercopulatory interval, ejaculation latency, and postejaculatory interval. Intromission frequency, intercopulatory interval, and ejaculation latency were decreased significantly by muscarine. Intrathecal homatropine decreased the number of copulating animals (five out of 13). In the five animals that were able to ejaculate after homatropine, intromission latency, intercopulatory interval, and ejaculation latency increased significantly. The effects of both drugs on locomotion were also tested. Muscarine induced no significant changes in locomotion compared with saline. A significant increase in locomotion was found after homatropine treatment. These results suggest that acetylcholine, acting at spinal-cord muscarinic receptors, may be involved in ejaculation.
authors.affiliation: Centro de Investigación en Reproducción Animal, Universidad Autónoma de Tlaxcala, Mexico.
authors.forename: I
authors.initials: I
authors.lastname: Durán
authors.forename: L
authors.initials: L
authors.lastname: Gil
authors.forename: R
authors.initials: R
authors.lastname: Cueva-Rolón
mesh_terms: D000818:Animals; D003307:Copulation / Q000187:drug effects* / Q000502:physiology; D004542:Ejaculation / Q000187:drug effects / Q000502:physiology; D007278:Injections, Spinal; D008297:Male; D009043:Motor Activity / Q000187:drug effects* / Q000502:physiology; D009116:Muscarine / Q000008:administration & dosage / Q000494:pharmacology*; D010276:Parasympatholytics / Q000008:administration & dosage / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D011930:Reaction Time; D013116:Spinal Cord / Q000187:drug effects / Q000502:physiology; D014326:Tropanes / Q000008:administration & dosage / Q000494:pharmacology
chemical_list: D010276:Parasympatholytics; D014326:Tropanes; D009116:Muscarine; C100236:homatropine