title: Localization of voltage-gated K(+) channels in squid giant axons.
abstract: We have localized the classical voltage-gated K(+) channel within squid giant axons by immunocytochemistry using the Kv1 antibody of Rosenthal et al. (1996). Widely dispersed patches of intense immunofluorescence were observed in the axonal membrane. Punctate immunofluorescence was also observed in the axoplasm and was localized to approximately 25-50-microm-wide column down the length of the nerve (axon diameter approximately 500 microm). Immunoelectronmicroscopy of the axoplasm revealed a K(+) channel containing vesicles, 30-50 nm in diameter, within this column. These and other vesicles of similar size were isolated from axoplasm using a novel combination of high-speed ultracentrifugation and controlled-pore size, glass bead separation column techniques. Approximately 1% of all isolated vesicles were labeled by K(+) channel immunogold reacted antibody. Incorporation of isolated vesicle fractions within an artificial lipid bilayer revealed K(+) channel electrical activity similar to that recorded directly from the axonal membrane by Llano et al. (1988). These K(+) channel-containing vesicles may be involved in cycling of K(+) channel protein into the axonal membrane. We have also isolated an axoplasmic fraction containing approximately 150-nm-diameter vesicles that may transport K(+) channels back to the cell body.
authors.affiliation: Laboratory of Neurophysiology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892, USA. jrclay@ninds.nih.gov
authors.forename: J R
authors.initials: JR
authors.lastname: Clay
authors.forename: A M
authors.initials: AM
authors.lastname: Kuzirian
mesh_terms: D000818:Animals; D000918:Antibody Specificity / Q000502:physiology*; D001369:Axons / Q000378:metabolism* / Q000648:ultrastructure*; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D048429:Cell Size / Q000502:physiology*; D003593:Cytoplasm / Q000378:metabolism / Q000648:ultrastructure; D049832:Decapodiformes / Q000166:cytology* / Q000378:metabolism*; D005456:Fluorescent Dyes; D008051:Lipid Bilayers / Q000378:metabolism; D015221:Potassium Channels / Q000276:immunology / Q000378:metabolism*; D022161:Transport Vesicles / Q000378:metabolism / Q000648:ultrastructure; D014966:Xanthenes
chemical_list: D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D015221:Potassium Channels; D014966:Xanthenes; C034657:Texas red