title: Characterization of a divergent vanD-type resistance element from the first glycopeptide-resistant strain of Enterococcus faecium isolated in Brazil.
abstract: Enterococcus faecium 10/96A from Brazil was resistant to vancomycin (MIC, 256 microg/ml) but gave no amplification products with primers specific for known van genotypes. A 2,368-bp fragment of a van cluster contained one open reading frame encoding a peptide with 83% amino acid identity to VanH(D), and a second encoding a D-alanine-D-lactate ligase with 83 to 85% identity to VanD. The divergent glycopeptide resistance phenotype was designated VanD4.
authors.affiliation: Antibiotic Resistance Monitoring and Reference Laboratory, Central Public Health Laboratory, London NW9 5HT, United Kingdom.
authors.forename: L M
authors.initials: LM
authors.lastname: Dalla Costa
authors.forename: P E
authors.initials: PE
authors.lastname: Reynolds
authors.forename: H A
authors.initials: HA
authors.lastname: Souza
authors.forename: D C
authors.initials: DC
authors.lastname: Souza
authors.forename: M F
authors.initials: MF
authors.lastname: Palepou
authors.forename: N
authors.initials: N
authors.lastname: Woodford
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D001938:Brazil; D004352:Drug Resistance, Microbial / Q000235:genetics; D016984:Enterococcus faecium / Q000187:drug effects / Q000235:genetics*; D014644:Genetic Variation; D005838:Genotype; D006020:Glycopeptides; D006801:Humans; D008969:Molecular Sequence Data; D010453:Peptide Synthases; D017386:Sequence Homology, Amino Acid
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D006020:Glycopeptides; D010453:Peptide Synthases; C122181:VanD protein, Enterococcus faecium