title: Rat cytomegalovirus replication in the salivary glands is exclusively confined to striated duct cells.
abstract: The salivary gland is the preferred organ for cytomegalovirus (CMV) replication and viral persistence. In order to identify the nature of infected cells and to study viral replication in more detail, several experiments were conducted. Using the rat CMV (RCMV) model, acutely infected young adult rats (6 weeks of age) and new-born rats (3 days of age) were infected, and submandibular, parotid and sublingual glands were harvested at different time points after infection. For identification of the nature of infected cells, immunohistochemistry, in situ hybridisation and electron microscopic techniques were used. In young adult animals, the submandibular gland was the preferred organ for RCMV replication. The parotid and sublingual glands contained fewer viruses than the submandibular gland. In contrast, in new-born rats, the main site of RCMV replication was the sublingual gland, while the submandibular and parotid glands contained low amounts of virus. No virus could be detected in the parotid glands. In all glands of RCMV-infected animals, the infection was exclusively confined to striated duct cells. Infection resulted in a cellular inflammatory response which was mostly located in the interlobular duct region, whereas only few inflammatory cells were found in the neighbourhood of infected striated duct cells. This phenomenon may contribute to the long persistence of the virus in this organ.
authors.affiliation: Department of Medical Microbiology, University Hospital Maastricht, The Netherlands.
authors.forename: J S
authors.initials: JS
authors.lastname: Kloover
authors.forename: J L
authors.initials: JL
authors.lastname: Hillebrands
authors.forename: G
authors.initials: G
authors.lastname: de Wit
authors.forename: G
authors.initials: G
authors.lastname: Grauls
authors.forename: J
authors.initials: J
authors.lastname: Rozing
authors.forename: C A
authors.initials: CA
authors.lastname: Bruggeman
authors.forename: P
authors.initials: P
authors.lastname: Nieuwenhuis
mesh_terms: D000818:Animals; D003587:Cytomegalovirus / Q000302:isolation & purification* / Q000502:physiology; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D011917:Rats, Inbred Lew; D012469:Salivary Glands / Q000648:ultrastructure / Q000821:virology*; D014779:Virus Replication