Timing of CNS cell generation: a programmed sequence of neuron and glial cell production from isolated murine cortical stem cells.
Multipotent stem cells that generate both neurons and glia are widespread components of the early neuroepithelium. During CNS development, neurogenesis largely precedes gliogenesis: how is this timing achieved? Using clonal cell culture combined with long-term time-lapse video microscopy, we show that isolated stem cells from the embryonic mouse cerebral cortex exhibit a distinct order of cell-type production: neuroblasts first and glioblasts later. This is accompanied by changes in their capacity to make neurons versus glia and in their response to the mitogen EGF. Hence, multipotent stem cells alter their properties over time and undergo distinct phases of development that play a key role in scheduling production of diverse CNS cells.
Qian
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X
Department of Pharmacology and Neuroscience, Albany Medical College, New York 12208, USA.
Shen
Q
Q
Goderie
S K
SK
He
W
W
Capela
A
A
Davis
A A
AA
Temple
S
S
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D019070:Cell Lineage; D002469:Cell Separation; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology* / Q000196:embryology / Q000254:growth & development; D002999:Clone Cells / Q000166:cytology / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D051379:Mice; D018715:Microscopy, Video; D009457:Neuroglia / Q000166:cytology*; D009474:Neurons / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects; D013997:Time Factors
D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor