The major tegumental antigen of Fasciola hepatica contains repeated elements.
In order to provide a better understanding of the interaction between the liver fluke (Fasciola hepatica) and the immune system of its mammalian host immunoreactive lambda bacteriophage clones containing F. hepatica cDNA have been isolated. Plasmids from these clones were sequenced and found to encode a family of proteins containing certain common elementst. All the clones contained a coding repeating sequence (RRRXCA) which is conserved at the nucleic acid level followed by a non-repeating element coding for the C terminal used by the proteins which shows conservation of amino acids at certain positions. Antisera raised against a beta-galactosidase fusion protein with one of these sequences as a terminal extension was used to localize the immunoreactive antigens. Binding was predominantly in the tegument of the juvenile fluke but was reduced in the adult tegument. The wall of the uterus showed strong reactivity in the adult. Rats immunized with the beta-galactosidase fusion protein showed enhanced resistance to challenge infections. The role of these antigens in the host response to infection by F. hepatica is discussed.
Trudgett
A
A
School of Biology and Biochemistry, The Queen's University of Belfast, N. Ireland. a.trudgett@qub.ac.uk
McNair
A T
AT
Hoey
E M
EM
Keegan
P S
PS
Dalton
J P
JP
Rima
B K
BK
Miller
A
A
Ramasamy
P
P
D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry* / Q000235:genetics; D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D005210:Fasciola hepatica / Q000235:genetics / Q000276:immunology*; D015723:Gene Library; D007150:Immunohistochemistry; D008099:Liver / Q000469:parasitology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D018510:RNA, Helminth / Q000737:chemistry / Q000302:isolation & purification; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D017422:Sequence Analysis, DNA; D018709:Statistics, Nonparametric
D000947:Antigens, Helminth; D018510:RNA, Helminth; D011993:Recombinant Fusion Proteins