title: The derivative spectrophotometric and IP-RP-HPLC determination of the Lometazid tablets.
abstract: In this paper the second-derivative spectrophotometric and ion-pair reversed-phase high performance liquid chromatographic methods for the simultaneous determination of some diuretic ingredients are described. Optimal conditions of both techniques for the quantitative analysis of the two-component diuretic mixture of the Lometazid tablets were settled. The second-derivative order of the spectra in sodium hydroxide with the wavelength modulation was used. For the determination of amiloride hydrochloride the 'zero-crossing' technique was applied, but for methyclothiazide the peak amplitude had to be corrected. In the ion-pair RP-HPLC technique tetrabutylammonium hydroxide was applied as an ion-pairing agent using acetonitrile-phosphate buffer (45:55 v/v) as a mobile phase. The validation was done of both proposed methods.
authors.affiliation: Department of Pharmaceutical Chemistry and Drug Analysis, Faculty of Pharmacy, University of Belgrade, Yugoslavia.
authors.forename: D
authors.initials: D
authors.lastname: Ivanović
authors.forename: M
authors.initials: M
authors.lastname: Medenica
authors.forename: S
authors.initials: S
authors.lastname: Kojić-Marinković
authors.forename: D
authors.initials: D
authors.lastname: Radulović
mesh_terms: D000584:Amiloride / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004232:Diuretics / Q000032:analysis*; D004338:Drug Combinations; D008736:Methyclothiazide / Q000032:analysis*; D013053:Spectrophotometry / Q000379:methods*; D013607:Tablets
chemical_list: D004232:Diuretics; D004338:Drug Combinations; C421266:Lometazid; D013607:Tablets; D000584:Amiloride; D008736:Methyclothiazide