A potential role for endothelin-1 in peripheral vascular disease.
The distribution of endothelin-1 (ET-1) and its receptors (ET(A)/ET(B)) has been studied in segments of femoral artery obtained from patients undergoing operation for peripheral vascular disease (PVD) using a combination of immunohistochemistry and autoradiography. Both receptor subtypes were located on the tunica media of vessel segments, with ET(A)-receptors predominating. Densitometric analysis showed that there was no difference in receptor binding to proximal/distal arterial segments from PVD patients. High-resolution autoradiography identified ET(B) binding to vascular nerves and vasa vasorum, mainly in distal portions of femoral arteries. Immunoreactive ET-1 was also identified that was associated with the vasa vasorum.
Dashwood
M R
MR
Department of Molecular Pathology & Clinical Biochemistry, Royal Free & University College Medical School, London, UK.
Jagroop
I A
IA
Gorog
D A
DA
Bagger
J P
JP
D001345:Autoradiography; D019332:Endothelin-1 / Q000032:analysis / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D016491:Peripheral Vascular Diseases / Q000188:drug therapy / Q000209:etiology*; D044022:Receptor, Endothelin A; D044023:Receptor, Endothelin B; D017466:Receptors, Endothelin / Q000502:physiology
D019332:Endothelin-1; D044022:Receptor, Endothelin A; D044023:Receptor, Endothelin B; D017466:Receptors, Endothelin