The structure of the carbohydrate backbone of the core-lipid A region of the lipopolysaccharide from Proteus vulgaris serotype O25.
The following structure of the lipid A-core region of the lipopolysaccharide (LPS) from Proteus vulgaris serotype O25 was determined by using NMR and chemical analysis of the core oligosaccharide, obtained by mild acid hydrolysis of LPS, of the products of alkaline deacylation of the LPS, and of the products of LPS deamination: [structure: see text] Terminal residues of beta-GlcNAc and beta-Kdo (indicated by bold italics) are present alternatively in approximately 3:2 amount, leaving no unsubstituted beta-Gal. All sugars are in the pyranose form, alpha-Hep is the residue of L-glycero-alpha-D-manno-Hep, alpha-DDHep is the residue of D-glycero-alpha-D-manno-Hep.
Vinogradov
E
E
Institute for Biological Sciences, National Research Council, Ottawa, Ont., Canada. evguenii.vinogradov@nrc.ca
Cedzynski
M
M
Rozalski
A
A
Ziolkowski
A
A
Swierzko
A
A
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D008050:Lipid A / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000737:chemistry*; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D011514:Proteus vulgaris / Q000737:chemistry*; D017421:Sequence Analysis; D012703:Serotyping
D008050:Lipid A; D008070:Lipopolysaccharides