The light chain composition of chicken brain myosin-Va: calmodulin, myosin-II essential light chains, and 8-kDa dynein light chain/PIN.
Class V myosins are a ubiquitously expressed family of actin-based molecular motors. Biochemical studies on myosin-Va from chick brain indicate that this myosin is a two-headed motor with multiple calmodulin light chains associated with the regulatory or neck domain of each heavy chain, a feature consistent with the regulatory effects of Ca(2+) on this myosin. In this study, the identity of three additional low molecular weight proteins of 23-,17-, and 10 kDa associated with myosin-Va is established. The 23- and 17-kDa subunits are both members of the myosin-II essential light chain gene family, encoded by the chicken L23 and L17 light chain genes, respectively. The 10-kDa subunit is a protein originally identified as a light chain (DLC8) of flagellar and axonemal dynein. The 10-kDa subunit is associated with the tail domain of myosin-Va.
Espindola
F S
FS
Department of Molecular, Cellular, Developmental Biology, Yale University, New Haven, Connecticut 06520-8103, USA.
Suter
D M
DM
Partata
L B
LB
Cao
T
T
Wolenski
J S
JS
Cheney
R E
RE
King
S M
SM
Mooseker
M S
MS
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002147:Calmodulin / Q000737:chemistry*; D002154:Calpain / Q000494:pharmacology; D002352:Carrier Proteins / Q000737:chemistry*; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D029721:Drosophila Proteins; D004398:Dyneins; D004591:Electrophoresis, Polyacrylamide Gel; D005407:Flagella / Q000737:chemistry; D005727:Ganglia, Spinal / Q000737:chemistry; D007074:Immunoglobulin G / Q000737:chemistry; D007381:Intermediate Filament Proteins / Q000737:chemistry* / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D018995:Myosin Heavy Chains; D018994:Myosin Light Chains / Q000737:chemistry* / Q000378:metabolism; D024701:Myosin Type V; D009218:Myosins / Q000737:chemistry*; D009474:Neurons / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D020539:Sequence Analysis, Protein
D002147:Calmodulin; D002352:Carrier Proteins; D029721:Drosophila Proteins; D007074:Immunoglobulin G; D007381:Intermediate Filament Proteins; C513082:Myo5a protein, mouse; D018994:Myosin Light Chains; D002154:Calpain; D024701:Myosin Type V; D018995:Myosin Heavy Chains; D009218:Myosins; D004398:Dyneins