title: Limited caspase cleavage of human BAP31.
abstract: Human BAP31 was cleaved at both of its two identical caspase cleavage sites in two previously reported models of apoptosis. We show here that only the most carboxy-terminal site is cleaved during apoptosis induced in HeLa cells by tunicamycin, tumor necrosis factor and cycloheximide, or staurosporine. Similar results were obtained in HL-60 cells using Fas/APO-1 antibodies, or cycloheximide. This limited cleavage, which is inhibited by several caspase inhibitors, removes eight amino acids from human BAP31 including the KKXX coat protein I binding motif. Ectopic expression of the resulting cleavage product induces redistribution of mannosidase II from the Golgi and prevents endoplasmic reticulum to Golgi transport of virus glycoproteins.
authors.affiliation: Department of Biosciences, Division of Biochemistry, Viikki Biocenter, Helsinki, Finland.
authors.forename: J
authors.initials: J
authors.lastname: Määttä
authors.forename: O
authors.initials: O
authors.lastname: Hallikas
authors.forename: S
authors.initials: S
authors.lastname: Welti
authors.forename: P
authors.initials: P
authors.lastname: Hildén
authors.forename: J
authors.initials: J
authors.lastname: Schröder
authors.forename: E
authors.initials: E
authors.lastname: Kuismanen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D001665:Binding Sites; D020169:Caspases / Q000378:metabolism*; D003513:Cycloheximide / Q000494:pharmacology; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008361:Mannosidases / Q000378:metabolism; D008565:Membrane Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D021381:Protein Transport; D011506:Proteins / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D019311:Staurosporine / Q000494:pharmacology; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014415:Tunicamycin / Q000494:pharmacology; D019014:fas Receptor / Q000276:immunology / Q000502:physiology
chemical_list: D000911:Antibodies, Monoclonal; C091247:BCAP31 protein, human; C489642:Bcap31 protein, mouse; D008565:Membrane Proteins; D011506:Proteins; D011993:Recombinant Fusion Proteins; D014409:Tumor Necrosis Factor-alpha; D019014:fas Receptor; D014415:Tunicamycin; D003513:Cycloheximide; D008361:Mannosidases; C038829:mannosyl-oligosaccharide 1,3 - 1,6-alpha-mannosidase; D020169:Caspases; D019311:Staurosporine