title: Seven genes that are differentially transcribed in colorectal tumor cell lines.
abstract: To identify genes which are differentially transcribed in colorectal tumor cells, we compared the two human tumor cell lines, SW480 and HCT116, with the cell line, NCM460, from normal colon epithelium as a control. Using the methods of differential display reverse transcription PCR and Northern blot hybridization, we detected the differential transcription of seven genes: cholecystokinin, reticulocalbin, Rab5 guanine nucleotide exchange factor Rabex5, caldesmon, differentiation related gene 1 (drg1), taxol resistant associated gene 3 (Trag-3) and the gene for the placental protein, diff33. The yet unidentified cDNA of the human Rabex5 gene and the 3' untranslated region of the human caldesmon gene were cloned.
authors.affiliation: Arbeitsgruppe Tumorgenetik, Abteilung Strukturelle Biologie, Max-Planck-Institut für molekulare Physiologie, Otto-Hahn-Strasse 11, D-44227, Dortmund, Germany.
authors.forename: I
authors.initials: I
authors.lastname: Nimmrich
authors.forename: S
authors.initials: S
authors.lastname: Erdmann
authors.forename: U
authors.initials: U
authors.lastname: Melchers
authors.forename: U
authors.initials: U
authors.lastname: Finke
authors.forename: S
authors.initials: S
authors.lastname: Hentsch
authors.forename: M P
authors.initials: MP
authors.lastname: Moyer
authors.forename: I
authors.initials: I
authors.lastname: Hoffmann
authors.forename: O
authors.initials: O
authors.lastname: Müller
mesh_terms: D000595:Amino Acid Sequence; D002135:Calcium-Binding Proteins / Q000235:genetics; D002148:Calmodulin-Binding Proteins / Q000235:genetics; D018797:Cell Cycle Proteins / Q000235:genetics; D002766:Cholecystokinin / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008562:Membrane Glycoproteins; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics; D011506:Proteins / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured; D000099259:N-myc Downstream-Regulated Gene 1 Protein
chemical_list: D002135:Calcium-Binding Proteins; D002148:Calmodulin-Binding Proteins; D018797:Cell Cycle Proteins; D002766:Cholecystokinin; D020662:Guanine Nucleotide Exchange Factors; D047908:Intracellular Signaling Peptides and Proteins; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins; D011506:Proteins; D012334:RNA, Neoplasm; D011956:Receptors, Cell Surface; D000099259:N-myc Downstream-Regulated Gene 1 Protein; C118809:CSAG2 protein, human; C108382:RABGEF1 protein, human; C103949:SERINC3 protein, human; C078865:reticulocalbin