title: Triggering CD101 molecule on human cutaneous dendritic cells inhibits T cell proliferation via IL-10 production.
abstract: Since the CD101 molecule is expressed on a major subpopulation of HLA-DR(+), CD1a(+), CD1c(+) cutaneous dendritic cells (DC), we studied the functional role of CD101 on cutaneous DC. Anti-CD101 monoclonal antibody (mAb) inhibited the proliferation of T cells induced by cutaneous DC. There was a synergistic inhibition between anti-CD101 mAb and anti-CD86/anti-CD80 mAb. Anti-CD101 mAb exerted its inhibitory effect when binding to the CD101 expressed on cutaneous DC. No positive role of CD101 putative ligand expressed by T cells in T cell proliferation was demonstrated, as T cells proliferated in response to soluble anti-CD3 mAb in the presence of CD86-transfected cells but not in the presence of CD101-transfected cells. Of major significance is the fact that IL-10 was produced by cutaneous DC after CD101 triggering with anti-CD101 mAb, while IL-10 secretion was up-regulated in mixed cutaneous DC-T cell cultures after CD101 triggering. Furthermore, IL-10-neutralizing mAb could reverse the inhibition induced by anti-CD101 mAb. Our results demonstrate that the CD101 triggering on cutaneous DC inhibits T cell proliferation via IL-10 production, suggesting an important regulatory role played by the CD101 molecule on DC during T cell activation.
authors.affiliation: INSERM U448, Paris XII University, Créteil, France. bouloc@univ-paris12.fr
authors.forename: A
authors.initials: A
authors.lastname: Bouloc
authors.forename: M
authors.initials: M
authors.lastname: Bagot
authors.forename: S
authors.initials: S
authors.lastname: Delaire
authors.forename: A
authors.initials: A
authors.lastname: Bensussan
authors.forename: L
authors.initials: L
authors.lastname: Boumsell
mesh_terms: D000818:Animals; D015703:Antigens, CD; D002450:Cell Communication / Q000276:immunology*; D002455:Cell Division / Q000276:immunology; D002460:Cell Line; D003713:Dendritic Cells / Q000276:immunology*; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis / Q000276:immunology*; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000276:immunology*; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D015703:Antigens, CD; C093213:CD101 antigen, human; D008562:Membrane Glycoproteins; D016753:Interleukin-10