Rapid dye decolorization method for screening potential wood preservatives.
We developed a new screening method for potential wood preservatives based on decolorization of the dye Remazol Brilliant Blue R by extracellular oxidative agents produced by wood decay fungi. Oxidative biodegradation of lignin yielded decolorized zones around and under fungal cultures on a dyed agar medium. Inhibitory effects were detected by direct observation and measurement of the decolorized zones.
Borokhov
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O
Lonzagroup, Research and Development Laboratories, Annandale, New Jersey 08801, USA. oborokhov@lonza-us.com
Rothenburger
S
S
D000880:Anthraquinones; D001673:Biodegradation, Environmental; D003116:Color; D004396:Coloring Agents; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005658:Fungi / Q000378:metabolism; D008031:Lignin / Q000378:metabolism; D014934:Wood
D000880:Anthraquinones; D004396:Coloring Agents; D008031:Lignin; C024758:Remazol Brilliant Blue R