Fetal microchimerism in primary biliary cirrhosis.
OBJECTIVE
Recent studies have suggested a role of fetal microchimerism in the pathogenesis of scleroderma. The present study investigated the potential role of fetal microchimerism in primary biliary cirrhosis (PBC), a closely related disease.


METHODS
A quantitative nested polymerase chain reaction was used to detect Y-chromosome sequences in the peripheral blood or the liver of PBC women and controls having male children and no transfusion or miscarriage history.


RESULTS
Male microchimerism was found in the peripheral blood from 45% (9 of 20) of PBC women and 25% (5 of 20) of healthy controls matched for the number of male children and age of the youngest son (p=0.28), and in the liver-biopsy specimens from 33% (5 of 15) of PBC women and 32% (8 of 25) of controls. The level of chimerism did not differ between patients and controls either in blood or in liver. Microchimerism was not related to the severity of the disease but was more frequent in PBC patients with anticentromere antibodies (p=0.049).


CONCLUSIONS
Fetal microchimerism does not seem to play a major role in most cases of PBC. However, the association with anticentromere antibodies suggests a possible role in the subgroup of patients with CREST syndrome or scleroderma.
Corpechot
C
C
Service d'Hepato-Gastro-Entérologie, Hôpital Saint-Antoine, Paris, France. corpechot@st-antoine.inserm.fr
Barbu
V
V
Chazouillères
O
O
Poupon
R
R
D000328:Adult; D000368:Aged; D000818:Animals; D017675:CREST Syndrome / Q000209:etiology; D002503:Centromere / Q000276:immunology; D002678:Chimera; D004247:DNA / Q000032:analysis*; D005260:Female; D006086:Graft vs Host Disease / Q000209:etiology; D006801:Humans; D008099:Liver / Q000473:pathology; D008105:Liver Cirrhosis, Biliary / Q000209:etiology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D014998:Y Chromosome
D004247:DNA