A rat model for investigation of bladder dysfunction associated with demyelinating disease resembling multiple sclerosis.
Myelin basic protein (MBP) can be used as an antigen for inducing experimental allergic encephalomyelitis (EAE). In various studies, EAE animals have been used as an experimental model of demyelinating diseases. The aim of this study was to determine whether EAE, induced by MBP in rats, can be useful for investigation of bladder dysfunction associated with demyelinating disease. Female Lewis rats were used. In Study 1, the time course of behavioral and cystometric changes were observed consecutively after MBP sensitization. In Study 2, the correlations between behavioral, cystometric, and histologic abnormalities were studied. The degree of paralysis and histologic findings were evaluated. In Study 1, transient hind limb paralysis was observed in all rats. Cystometric findings were characterized by three different patterns: 1) detrusor areflexia (DA), 2) detrusor hyperactivity (DH), and 3) normal. Ten (77%) of the 13 rats given MBP showed bladder dysfunction, including DA (seven), DA/DH (two) and DH (one). Study 2 showed DA in 10 rats, DH in one, and normal findings in nine animals. The difference in degree of paralysis between the DA and the cystometrically normal animals was statistically significant (P<0.01). The mean value of the degree of inflammation in the spinal cord (L6-S1) in the DA group was significantly (P<0.05) higher than that in the cystometrically normal group. The degrees of paralysis and spinal inflammation were weakly correlated (R = 0.47, P = 0.05). The present rat model seems useful for studies of bladder dysfunction associated with spinal myelitis/demyelinating diseases.
Mizusawa
H
H
Department of Urology, Shinshu University School of Medicine, Matsumoto, Japan. Hiroya.Mizusawa@klinfarm.lu.se
Igawa
Y
Y
Nishizawa
O
O
Ichikawa
M
M
Ito
M
M
Andersson
K E
KE
D000818:Animals; D003711:Demyelinating Diseases / Q000150:complications / Q000473:pathology / Q000503:physiopathology*; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000150:complications / Q000473:pathology / Q000503:physiopathology*; D005260:Female; D009103:Multiple Sclerosis / Q000150:complications / Q000503:physiopathology*; D051381:Rats; D011917:Rats, Inbred Lew; D001743:Urinary Bladder / Q000473:pathology / Q000503:physiopathology*; D001745:Urinary Bladder Diseases / Q000209:etiology / Q000473:pathology / Q000503:physiopathology*; D014563:Urodynamics / Q000502:physiology*