Bioactive cytidine deaminase, an inhibitor of granulocyte-macrophage colony-forming cells, is massively released in fulminant meningococcal sepsis.
Cytidine deaminase (CDD) catalyzes the hydrolytic deamination of cytidine, which thereby is converted to uridine. CDD is found in serum and different tissues, with particularly high concentrations in polymorphonuclear neutrophils (PMN). We measured the CDD levels in plasma from patients with systemic meningococcal disease. Thirty-seven patients had significantly higher plasma levels of CDD than did 29 healthy control subjects (P=.0001). CDD levels in plasma or serum increased from a median of 96 ng/mL in healthy control subjects to medians of 168 ng/mL in patients without persistent shock (n=23; P=.001) and 422 ng/mL in patients with fulminant meningococcal septicemia (n=14; P=.0001). In most patients with fulminant septicemia, CDD levels in plasma increased during the first 3-53 h after the initiation of therapy (P=.003). CDD alone had no immediate harmful effect when injected into mice during a 4-day period. CDD may modulate the stimulatory effect of colony-stimulating factors on PMN in patients.
Bøyum
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A
Nycomed Amersham Imaging A/S, Fou-II-3, N-0401 Oslo, Norway. arbo@nycomed.com
Tennfjord
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VA
Gran
C
C
Løvhaug
D
D
Oktedalen
O
O
Brandtzaeg
P
P
D000293:Adolescent; D000328:Adult; D000818:Animals; D016470:Bacteremia / Q000201:enzymology*; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000037:antagonists & inhibitors; D003564:Cytidine Deaminase / Q000097:blood*; D004211:Disseminated Intravascular Coagulation / Q000097:blood; D005260:Female; D006098:Granulocytes / Q000502:physiology; D006801:Humans; D008264:Macrophages / Q000502:physiology; D008585:Meningitis, Meningococcal / Q000097:blood / Q000201:enzymology; D008589:Meningococcal Infections / Q000097:blood / Q000201:enzymology*; D051379:Mice; D008810:Mice, Inbred C57BL; D012772:Shock, Septic / Q000097:blood
D003115:Colony-Stimulating Factors; D003564:Cytidine Deaminase