Genetic and biochemical characterization of dTOR, the Drosophila homolog of the target of rapamycin.
The adaptation of growth in response to nutritional changes is essential for the proper development of all organisms. Here we describe the identification of the Drosophila homolog of the target of rapamycin (TOR), a candidate effector for nutritional sensing. Genetic and biochemical analyses indicate that dTOR impinges on the insulin signaling pathway by autonomously affecting growth through modulating the activity of dS6K. However, in contrast to other components in the insulin signaling pathway, partial loss of dTOR function preferentially reduces growth of the endoreplicating tissues. These results are consistent with dTOR residing on a parallel amino acid sensing pathway.
Oldham
S
S
Zoologisches Institut, Universität Zürich, 8057 Zürich, Switzerland.
Montagne
J
J
Radimerski
T
T
Thomas
G
G
Hafen
E
E
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D004734:Energy Metabolism / Q000187:drug effects / Q000235:genetics*; D005656:Fungal Proteins / Q000502:physiology; D005804:Genes, Lethal; D019476:Insect Proteins / Q000032:analysis / Q000235:genetics* / Q000502:physiology; D007328:Insulin / Q000502:physiology; D047908:Intracellular Signaling Peptides and Proteins; D007814:Larva; D010448:Peptide Initiation Factors; D019869:Phosphatidylinositol 3-Kinases / Q000502:physiology; D010750:Phosphoproteins / Q000502:physiology; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000032:analysis / Q000235:genetics* / Q000502:physiology; D011494:Protein Kinases; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D019893:Ribosomal Protein S6 Kinases / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins; D015398:Signal Transduction; D020123:Sirolimus / Q000494:pharmacology*; D013045:Species Specificity; D058570:TOR Serine-Threonine Kinases
D000596:Amino Acids; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D029721:Drosophila Proteins; D005656:Fungal Proteins; D019476:Insect Proteins; D007328:Insulin; D047908:Intracellular Signaling Peptides and Proteins; D010448:Peptide Initiation Factors; D019869:Phosphatidylinositol 3-Kinases; D010750:Phosphoproteins; D017853:Phosphotransferases (Alcohol Group Acceptor); D011494:Protein Kinases; D011993:Recombinant Fusion Proteins; D019893:Ribosomal Protein S6 Kinases; D029701:Saccharomyces cerevisiae Proteins; D020123:Sirolimus; D058570:TOR Serine-Threonine Kinases; C410006:Thor protein, Drosophila; C416303:target of rapamycin protein, Drosophila; C083324:TOR1 protein, S cerevisiae; C081135:TOR2 protein, S cerevisiae