cDNA expression array analysis of DNA repair genes in human glioma cells that lack or express DNA-PK.
M059J cells provide the only example of DNA-PKcs (now known as PRKDC) deficiency in a human cell line. M059K cells, derived from the same tumor specimen, express PRKDC protein and activity and, together with M059J, provide a useful model in which to study the role of DNA-PK in cellular responses to DNA-damaging agents. Because these cells are of tumor origin, we used Atlas human cancer cDNA expression arrays to investigate possible differential expression of other DNA repair genes in control and irradiated samples. cDNA array results indicated differential expression of 14 genes. Northern blotting confirmed relatively greater expression of replication factor C 37-kDa subunit mRNA in M059J cells compared to M059K cells and reduced expression of DNA ligase IV compared to ligase III in both cell lines independent of irradiation. These results suggest that other DNA repair proteins are altered in these cell lines and that repair mechanisms predicted from the study of normal tissues may be fundamentally altered in human cancer cells.
Galloway
A M
AM
Department of Oncology, Cross Cancer Institute, Edmonton, Alberta, Canada.
Allalunis-Turner
J
J
D000199:Actins / Q000096:biosynthesis / Q000235:genetics; D001345:Autoradiography; D015152:Blotting, Northern; D002470:Cell Survival / Q000528:radiation effects; D000072481:DNA Ligase ATP; D011088:DNA Ligases / Q000096:biosynthesis / Q000235:genetics; D004260:DNA Repair / Q000235:genetics* / Q000528:radiation effects*; D051747:DNA-Activated Protein Kinase; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D005720:Gamma Rays; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects; D005910:Glioma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D018398:Homeodomain Proteins; D006801:Humans; D015778:Minor Histocompatibility Antigens; D008954:Models, Biological; D009687:Nuclear Proteins; D020411:Oligonucleotide Array Sequence Analysis; D000075223:Poly-ADP-Ribose Binding Proteins; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger / Q000378:metabolism; D051818:Replication Protein C; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D013482:Superoxide Dismutase / Q000096:biosynthesis / Q000235:genetics; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays; D029867:Xenopus Proteins
D000199:Actins; C096816:BCL2-related protein A1; D004268:DNA-Binding Proteins; C000607891:DNL4 protein, S cerevisiae; D018398:Homeodomain Proteins; C000607893:LIG4 protein, human; C096063:MATA1 protein, S cerevisiae; D015778:Minor Histocompatibility Antigens; D009687:Nuclear Proteins; D000075223:Poly-ADP-Ribose Binding Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D029867:Xenopus Proteins; D013482:Superoxide Dismutase; D051747:DNA-Activated Protein Kinase; C497263:PRKDC protein, human; D017346:Protein Serine-Threonine Kinases; D051818:Replication Protein C; D011088:DNA Ligases; D000072481:DNA Ligase ATP; C000618816:DNA ligase III alpha protein, Xenopus; C000618817:LIG3 protein, human