title: The GB virus C/hepatitis G virus replicates in hepatocytes without causing liver disease in healthy blood donors.
abstract: Thirty-seven persons were identified with GB virus C (GBV-C) single infection by polymerase chain reaction screening of 1254 healthy blood donors. Of 33 donors who returned for clinical examination, 17 underwent liver biopsy. Clinical, biochemical, and histologic evaluation did not reveal any signs of liver disease. Liver biopsies of 15 donors were analyzed by in situ hybridization with GBV-C RNA probes and immunologic staining for the GBV-C envelope 2 protein. GBV-C replication was identified in the cytoplasm of hepatocytes of 10 (67%) donor livers but in none of 7 liver biopsies of chronic hepatitis B virus carriers negative for serum GBV-C RNA. Thus, there was no evidence of liver disease in GBV-C-infected healthy blood donors despite viral replication in hepatocytes.
authors.affiliation: Division of Clinical Virology, Karolinska Institute at Huddinge University Hospital, Huddinge, Sweden.
authors.forename: R
authors.initials: R
authors.lastname: Halasz
authors.forename: M
authors.initials: M
authors.lastname: Sällberg
authors.forename: S
authors.initials: S
authors.lastname: Lundholm
authors.forename: G
authors.initials: G
authors.lastname: Andersson
authors.forename: B
authors.initials: B
authors.lastname: Lager
authors.forename: H
authors.initials: H
authors.lastname: Glaumann
authors.forename: O
authors.initials: O
authors.lastname: Weiland
mesh_terms: D000328:Adult; D001706:Biopsy; D001782:Blood Donors; D015331:Cohort Studies; D005260:Female; D018067:Flaviviridae / Q000235:genetics / Q000302:isolation & purification*; D006525:Hepatitis, Viral, Human / Q000097:blood / Q000473:pathology / Q000821:virology; D022781:Hepatocytes / Q000821:virology; D006801:Humans; D017403:In Situ Hybridization; D008099:Liver / Q000473:pathology / Q000821:virology*; D008297:Male; D008875:Middle Aged; D012367:RNA, Viral / Q000032:analysis / Q000097:blood; D019562:Viral Load; D014779:Virus Replication
chemical_list: D012367:RNA, Viral