Comparison of different protocols of MRC5 cell line permeabilization for detection of CMV IE antigens by flow cytometry.
The usefulness of four different protocols of permeabilizing human fibroblast cells for detection of CMV immediate-early antigens by flow cytometry was investigated. Permeabilizing protocols employed methanol, methanol/acetone, paraformaldehyde/Triton and FACSPermeabilizing Solution (Becton Dickinson). The best parameters (separation between positive stained CMV infected cells and negative control and detection of infected cells) were achieved for paraformaldehyde/Triton and FACSPermeabilizing Solution (Becton Dickinson).
Siennicka
J
J
Department of Virology, National Institute of Hygiene, Warsaw, Chocimska 24, Poland.
D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D003587:Cytomegalovirus / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D017874:Immediate-Early Proteins / Q000032:analysis*; D010539:Permeability
D000956:Antigens, Viral; D017874:Immediate-Early Proteins; C044582:immediate-early proteins, cytomegalovirus