Determination of H+/K+-ATPase activity in human gastric biopsy specimens.
The aim of our work was to develop a method to determine the the H+/K+-ATPase activity of human gastric biopsy samples. Our method is based on the phosphatase activity and the K+-inducible property of the enzyme. K+-inducible pNPPase activity was determined from homogenated corpus and antrum biopsy samples. H+/K+-ase activity was calculated as the difference between the corpus and antrum K+-inducible pNPPase activities. Quality control measurements were done during 20 successive days from pooled homogenates. The total, between-day and between-run, within-day and within-run coefficients of variations were between 10 and 16%. The healthy mean and reference range of K+-inducible pNPPase activity in the corpus was 95.8 (95% CI: 83.4-108.2 mU/mg protein); in the antrum it was 28.3 (21.6-35.0) mU/mg protein. The calculated H+/K+-ATPase activity was 67.2 (56.9-77.5) mU/mg protein. The measured activities were independent of the age and gender. Summarizing our results we have concluded, that our novel method might be a potential tool to gather data about the functional acid producing capability of human gastric mucosa.
Kocsis
I
I
1st Department of Pediatrics, Semmelweis University, Budapest, Hungary. kopist@gyer1.sote.hu
Vásárhelyi
B
B
Tulassay
Z
Z
Szabó
T
T
Vér
A
A
Tulassay
T
T
D000293:Adolescent; D000328:Adult; D000368:Aged; D000375:Aging; D001706:Biopsy; D002648:Child; D005260:Female; D005753:Gastric Mucosa / Q000201:enzymology*; D017506:H(+)-K(+)-Exchanging ATPase / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009596:Nitrophenols / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism; D011786:Quality Control; D012016:Reference Values; D015203:Reproducibility of Results; D012727:Sex Characteristics
D009596:Nitrophenols; D009943:Organophosphorus Compounds; C008644:nitrophenylphosphate; D017506:H(+)-K(+)-Exchanging ATPase