The open reading frame of the Na(+)-dependent glutamate transporter GLAST-1 is expressed in bone and a splice variant of this molecule is expressed in bone and brain.
Previously we have reported expression of an mRNA with homology to the Na(+)-dependent glutamate transporter, GLAST-1, in bone. Here we demonstrate that the complete open reading frame of GLAST-1 mRNA and corresponding 69 kDa protein are expressed in rat bone in vivo. We have also discovered a novel splice variant (GLAST-1a), lacking exon 3, expressed in rat bone and brain. A 55 kDa protein detected by anti-GLAST antibody in rat cerebellum corresponds to the molecular weight of unglycosylated GLAST-1a. This has led us to propose that GLAST-1a has an opposite orientation in the cell membrane to GLAST-1.
Huggett
J
J
School of Bioscience, Cardiff University, Museum Avenue, P.O. Box 911, Cardiff CF10 3US, UK.
Vaughan-Thomas
A
A
Mason
D
D
D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D017398:Alternative Splicing; D027322:Amino Acid Transport System X-AG; D000818:Animals; D001483:Base Sequence; D001842:Bone and Bones / Q000737:chemistry*; D001923:Brain Chemistry; D002531:Cerebellum / Q000737:chemistry; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D005091:Exons; D015870:Gene Expression; D006031:Glycosylation; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar
D018528:ATP-Binding Cassette Transporters; D027322:Amino Acid Transport System X-AG; D012333:RNA, Messenger; D015246:Deoxyribonuclease EcoRI