Multiparameter data acquisition and analysis of leukocytes by flow cytometry.
Each laboratory has to establish its own experience base and standard operating procedures. The intent of this discussion has been to illustrate the procedures that will lead to good flow cytometry data acquisition and analysis and to illustrate problematic areas. The most important rule of all is to recognize when there is a problem and find the correct solution. It is hoped the information provided herein will be of help in the recognition process.
Stewart
C C
CC
Laboratory of Flow Cytometry, Roswell Park Cancer Institute, Buffalo, New York 14263, USA.
Stewart
S J
SJ
D000906:Antibodies / Q000276:immunology / Q000378:metabolism; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods*; D005456:Fluorescent Dyes; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007963:Leukocytes, Mononuclear / Q000166:cytology*
D000906:Antibodies; D005456:Fluorescent Dyes