title: Intercept-PCR, an improvement for elevating performance to find a new member of a certain gene family.
abstract: We have established a method by which the performance of reverse transcriptase coupled polymerase chain reaction (RT-PCR) for seeking a new gene is improved. The actual procedure is quite easy: it is only to add several specific oligonucleotides into the reaction mixture of the usual RT-PCR. To verify the effectiveness of this method is also easy: it is only to detect the PCR products in the preliminary experiment. The finding in the present study provides valuable information for gene cloning tactics.
authors.affiliation: Nagoya University School of Medicine, Dep. Mol. Pathogen., Japan. smatsuda@med.nagoya-u.ac.jp
authors.forename: S
authors.initials: S
authors.lastname: Matsuda
authors.forename: Y
authors.initials: Y
authors.lastname: Ichigotani
authors.forename: T
authors.initials: T
authors.lastname: Okuda
authors.forename: K
authors.initials: K
authors.lastname: Miyazaki
authors.forename: T
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Nimura
authors.forename: T
authors.initials: T
authors.lastname: Irimura
authors.forename: S
authors.initials: S
authors.lastname: Nakatsugawa
authors.forename: M
authors.initials: M
authors.lastname: Hamaguchi
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers; D018076:DNA, Complementary; D006801:Humans; D005810:Multigene Family; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger