title: Frequency and spectrum of HPV types detected in cutaneous squamous-cell carcinomas depend on the HPV detection system: a comparison of four PCR assays.
abstract: BACKGROUND
  The association of human papillomavirus (HPV) with cutaneous squamous-cell carcinomas (SCCs) has been described recently, but the frequency and spectrum of HPV types identified differed substantially in distinct studies.
  
  
  OBJECTIVE
  Comparison of different PCR assays with respect to sensitivity and range of HPV types detected.
  
  
  METHODS
  Cutaneous SCC were analyzed for HPV DNA using both consensus PCR assays with degenerate primers and PCR assays with nondegenerate primers derived from HPV types 5 and 8.
  
  
  RESULTS
  HPV DNA was found in 50% of SCC specimens using degenerate primers. The rate of HPV-DNA-positive specimens increased to 69% when PCR assays with nondegenerate primers were applied in addition. The spectrum of HPV types detected with each of the PCR assays differed considerably.
  
  
  CONCLUSIONS
  The frequency and spectrum of HPV types detected in cutaneous SCC strongly depends on the HPV detection system used and urges the need for standardization of HPV detection and typing in skin lesions in order to characterize HPV types predominating in distinct tumors.
authors.affiliation: Institute of Immunology, Pathology and Molecular Biology, Hamburg, Germany. meyer@labor-keeser-arndt.de
authors.forename: T
authors.initials: T
authors.lastname: Meyer
authors.forename: R
authors.initials: R
authors.lastname: Arndt
authors.forename: E
authors.initials: E
authors.lastname: Christophers
authors.forename: E
authors.initials: E
authors.lastname: Stockfleth
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000821:virology*; D017931:DNA Primers; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics; D030361:Papillomavirus Infections / Q000473:pathology / Q000821:virology; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012878:Skin Neoplasms / Q000473:pathology / Q000821:virology*; D014412:Tumor Virus Infections / Q000473:pathology / Q000821:virology
chemical_list: D017931:DNA Primers; D004279:DNA, Viral