A novel "Nihon" rat model of a Mendelian dominantly inherited renal cell carcinoma.
A novel rat model of hereditary renal cell carcinoma (RC) was found in a rat colony of the Sprague-Dawley strain in Japan, and named the rising "Nihon" rat. In this strain, RCs develop from early preneoplastic lesions, which begin to appear at 4 weeks of age, forming adenomas by the age of 16 weeks. The RCs are predominantly of clear cell type. Southern blot, northern blot and SSCP analyses revealed no change in the Tsc1, Tsc2, VHL, and c-Met genes. Thus, the Nihon rat should be a valuable experimental model for understanding renal carcinogenesis, especially clear cell type, which is common among human RCs.
Okimoto
K
K
Department of Toxicology and Teratology, Developmental Research Laboratories, Dainippon Pharmaceutical Co., Ltd., Suita, Osaka 564-0053, Japan. kazuo-okimoto@dainippon-pharm.co.jp
Kouchi
M
M
Kikawa
E
E
Toyosawa
K
K
Koujitani
T
T
Tanaka
K
K
Matsuoka
N
N
Sakurai
J
J
Hino
O
O
D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D002292:Carcinoma, Renal Cell / Q000235:genetics* / Q000473:pathology; D003433:Crosses, Genetic; D004195:Disease Models, Animal; D005260:Female; D005799:Genes, Dominant / Q000235:genetics; D016147:Genes, Tumor Suppressor; D007680:Kidney Neoplasms / Q000235:genetics* / Q000473:pathology; D008025:Ligases; D008297:Male; D009154:Mutation; D010375:Pedigree; D010641:Phenotype; D018807:Polymorphism, Single-Stranded Conformational; D011506:Proteins / Q000235:genetics; D019859:Proto-Oncogene Proteins c-met / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012097:Repressor Proteins / Q000235:genetics; D000077004:Tuberous Sclerosis Complex 1 Protein; D000077005:Tuberous Sclerosis Complex 2 Protein; D025521:Tumor Suppressor Proteins; D044767:Ubiquitin-Protein Ligases; D051794:Von Hippel-Lindau Tumor Suppressor Protein
D011506:Proteins; D012097:Repressor Proteins; C000624650:TSC1 protein, human; C000624653:TSC2 protein, human; C000624652:Tsc1 protein, rat; C000624655:Tsc2 protein, rat; D000077004:Tuberous Sclerosis Complex 1 Protein; D000077005:Tuberous Sclerosis Complex 2 Protein; D025521:Tumor Suppressor Proteins; D044767:Ubiquitin-Protein Ligases; D051794:Von Hippel-Lindau Tumor Suppressor Protein; D019859:Proto-Oncogene Proteins c-met; D008025:Ligases