Protective effect of lycopene on lipid peroxidation and oxidative DNA damage in cell culture.
A high incidence of cancer has been correlated with chronic iron overload, and carotenoids are of interest as possible anticarcinogens. We have investigated the effect of lycopene on lipid peroxidation and on the formation of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) in CV1-P monkey cells exposed to ferric nitrilotriacetate (Fe-NTA) plus ascorbate. Cells supplemented with lycopene (20 pmol/10(6) cells) showed a reduction of 86% in Fe-NTA/ascorbate-induced lipid peroxidation (TBARS). Levels of 8-oxodGuo rose from 1.59+/-0.09 residues/10(6) dGuo in the control cells to 14.02+/-0.41 residues/10(6) dGuo after incubation with (1:4 mM) Fe-NTA/ascorbate (40 microM). Lycopene supplementation decreased in 77% the 8-oxodGuo levels in Fe-NTA/ascorbate-treated cells. These results indicate that lycopene can protect mammalian cells against membrane and DNA damage and possibly play a protective role against tumor promotion associated with oxidative damage.
Matos
H R
HR
Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, SP, Brazil.
Di Mascio
P
P
Medeiros
M H
MH
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D002338:Carotenoids / Q000378:metabolism / Q000494:pharmacology*; D002460:Cell Line; D004249:DNA Damage / Q000187:drug effects*; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D005290:Ferric Compounds / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects*; D000077276:Lycopene; D009153:Mutagens / Q000494:pharmacology; D009571:Nitrilotriacetic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects*
D000975:Antioxidants; D005290:Ferric Compounds; D009153:Mutagens; D002338:Carotenoids; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; D009571:Nitrilotriacetic Acid; D001205:Ascorbic Acid; D000077276:Lycopene; C020326:ferric nitrilotriacetate