Diversity of L-methionine catabolism pathways in cheese-ripening bacteria.
Enzymatic activities that could be involved in methanethiol generation in five cheese-ripening bacteria were assayed, and the major sulfur compounds produced were identified. L-Methionine and alpha-keto-gamma-methyl-thio-butyric acid demethiolating activities were detected in whole cells and cell extracts (CFEs) of all the bacteria tested. No L-methionine deaminase activity could be detected in any of the ripening bacteria and L-methionine aminotransferase was detected in CFEs of Brevibacterium linens, Micrococcus luteus, and Corynebacterium glutamicum. The results suggest that several pathways for L-methionine catabolism probably coexist in these ripening bacteria.
Bonnarme
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Laboratoire de Génie et Microbiologie des Procédés Alimentaires, Institut National de la Recherche Agronomique, Centre de Biotechnologies Argo-Industrielles, 78850 Thiverval-Grignon, France. bonnarme@grignon.inra.fr
Psoni
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L
Spinnler
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HE
D001173:Arthrobacter / Q000378:metabolism; D001419:Bacteria / Q000378:metabolism*; D001951:Brevibacterium / Q000378:metabolism; D013437:Carbon-Sulfur Lyases / Q000378:metabolism; D002611:Cheese / Q000382:microbiology*; D003352:Corynebacterium / Q000378:metabolism; D007700:Kinetics; D008715:Methionine / Q000378:metabolism*; D008837:Micrococcus / Q000378:metabolism; D008954:Models, Biological; D013210:Staphylococcus / Q000378:metabolism; D000637:Transaminases / Q000378:metabolism
D008715:Methionine; D000637:Transaminases; C020656:glutamine-pyruvate aminotransferase; D013437:Carbon-Sulfur Lyases; C021558:L-methionine gamma-lyase