title: Formation of extracellular structures containing VirB2 proteins in mating cultures of agrobacteria.
abstract: Using transmission electron immunomicroscopy, VirB2 protein has been revealed at the surface of acetosyringon-treated A. tumefaciens cells. VirB2 was seen within long flexible and short structures localized at the opposite poles of the cells. These structures were not observed in cells not treated with acetosyringon and in agrobacterial cells treated with this reagent but carrying no Ti-plasmid. Labeled complexes [antibodies to virB2 protein + (A protein + colloidal gold)] bound to pili at a certain periodicity.
authors.affiliation: Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov.
authors.forename: I V
authors.initials: IV
authors.lastname: Kurbanova
authors.forename: M I
authors.initials: MI
authors.lastname: Chumakov
mesh_terms: D000098:Acetophenones; D016960:Agrobacterium tumefaciens / Q000378:metabolism / Q000648:ultrastructure*; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000378:metabolism / Q000648:ultrastructure*; D008565:Membrane Proteins / Q000378:metabolism / Q000648:ultrastructure; D037521:Virulence Factors
chemical_list: D000098:Acetophenones; D001426:Bacterial Proteins; D008565:Membrane Proteins; D037521:Virulence Factors; C051667:acetosyringone