Studies on the interaction between TWEAK and the death receptor WSL-1/TRAMP (DR3).
WSL-1/TRAMP (DR3) is a member of the tumour necrosis factor (TNF) receptor superfamily which exhibits effects on NF-kappaB activation and apoptosis. TWEAK, a novel TNF-related molecule, has been proposed as the ligand for this receptor. Utilising both human and murine TWEAK ligand, it is shown that TWEAK and WSL-1/TRAMP do not interact in an in vitro binding assay and that TWEAK binds strongly to cells that do not express WSL-1/TRAMP on the cell surface. Biological activity of TWEAK is also observed in these cells. Finally, cells isolated from WSL-1/TRAMP knockout mice are shown to retain their ability to interact with TWEAK. These results suggest that WSL-1/TRAMP is not the major receptor for TWEAK
Kaptein
A
A
Cell Biology Department, GlaxoWellcome Medicines Research Centre, Stevenage, UK.
Jansen
M
M
Dilaver
G
G
Kitson
J
J
Dash
L
L
Wang
E
E
Owen
M J
MJ
Bodmer
J L
JL
Tschopp
J
J
Farrow
S N
SN
D000818:Animals; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins; D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D000074049:Cytokine TWEAK; D006801:Humans; D016209:Interleukin-8 / Q000494:pharmacology; D008214:Lymphocytes / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D016328:NF-kappa B / Q000378:metabolism; D018124:Receptors, Tumor Necrosis Factor / Q000172:deficiency / Q000378:metabolism*; D053219:Receptors, Tumor Necrosis Factor, Member 25; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D048069:Tumor Necrosis Factors
D051017:Apoptosis Regulatory Proteins; D002352:Carrier Proteins; D000074049:Cytokine TWEAK; D016209:Interleukin-8; D016328:NF-kappa B; D018124:Receptors, Tumor Necrosis Factor; D053219:Receptors, Tumor Necrosis Factor, Member 25; C506033:TNFRSF25 protein, human; C109743:TNFSF12 protein, human; C506034:Tnfrsf25 protein, mouse; C491645:Tnfsf12 protein, mouse; D014409:Tumor Necrosis Factor-alpha; D048069:Tumor Necrosis Factors