The antiviral compound 5-(3,4-dichlorophenyl) methylhydantoin inhibits the post-synthetic cleavages and the assembly of poliovirus in a cell-free system.
The mode of action of the antiviral drug 5-(3,4-dichlorophenyl) methylhydantoin (hydantoin) was studied in a cell-free system allowing de novo synthesis of poliovirus. This cell-free system, which is programmed with viral RNA, is suitable for the study of the late stages of poliovirus replication and, thus, for a study of antiviral compounds acting on these late stages. It was shown that, apart from the known inhibition of the assembly of poliovirus, hydantoin also blocks post-synthetic cleavages of poliovirus proteins. Our data demonstrate that the cell-free system is a sensitive tool to study the mode of action of antiviral compounds.
Verlinden
Y
Y
Department of Microbiology and Hygiene, Vrije Universiteit Brussel, Laarbeeklaan 103, B-1090, Brussels, Belgium.
Cuconati
A
A
Wimmer
E
E
Rombaut
B
B
D000998:Antiviral Agents / Q000494:pharmacology*; D002474:Cell-Free System; D006367:HeLa Cells; D006801:Humans; D006827:Hydantoins / Q000494:pharmacology*; D011051:Poliomyelitis / Q000821:virology; D017955:Poliovirus / Q000187:drug effects* / Q000502:physiology*; D014771:Virion / Q000378:metabolism; D019065:Virus Assembly / Q000187:drug effects*; D014779:Virus Replication / Q000187:drug effects
C424268:5-(3,4-dichlorophenyl)methylhydantoin; D000998:Antiviral Agents; D006827:Hydantoins