title: Cytotoxicity, blood compatibility and antimicrobial activity of two cyanoacrylate glues for surgical use.
abstract: The biocompatibility of two cyanoacrylate surgical glues (Glubran and Glubran 2), supplied by General Enterprise Marketing, Viareggio, Lucca, Italy, was tested through cytotoxicity and blood compatibility tests and the evaluation of antimicrobial activity. Cytotoxicity and blood compatibility tests were performed on the polymerized glues. Using the neutral red uptake test, the extracts from Glubran and Glubran 2 after polymerization were non-toxic to L929 cells only when diluted 1: 10 with culture medium. Glubran and Glubran 2 induced a significant decrease of activated partial thromboplastin time (APTT), which is favourable with regard to the desired haemostasis. The APTT shortening determines a haemostatic effect and therefore contribute to the tissue adhesion induced by the glues. Otherwise, no significant variation of prothrombin activity, fibrinogen, platelet number, total and differential leukocyte count was induced by the glues, which, in addition, did not show haemolytic effect. There was no difference between Glubran and Glubran 2 regarding haemocompatibility. The antimicrobial ability of the unpolymerized glues was tested onto Bacillus subtilis var. niger for 3 weeks: neither Glubran nor Glubran 2 were found effective in this respect. In conclusion, we can assume that cytotoxicity was severe with the undiluted glues, but was acceptable when glues were diluted. On the contrary, blood compatibility was acceptable for the intended use of the glues. No difference was found between Glubran and Glubran 2 after polymerization.
authors.affiliation: Laboratory for Biocompatibility Research on Implant Materials, Istituti Ortopedici Rizzoli, Bologna, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Montanaro
authors.forename: C R
authors.initials: CR
authors.lastname: Arciola
authors.forename: E
authors.initials: E
authors.lastname: Cenni
authors.forename: G
authors.initials: G
authors.lastname: Ciapetti
authors.forename: F
authors.initials: F
authors.lastname: Savioli
authors.forename: F
authors.initials: F
authors.lastname: Filippini
authors.forename: L A
authors.initials: LA
authors.lastname: Barsanti
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003487:Cyanoacrylates / Q000494:pharmacology*; D005340:Fibrinogen / Q000187:drug effects / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects; D006487:Hemostasis / Q000187:drug effects*; D006801:Humans; D051379:Mice; D010314:Partial Thromboplastin Time; D011516:Prothrombin / Q000187:drug effects / Q000378:metabolism
chemical_list: D001672:Biocompatible Materials; D003487:Cyanoacrylates; C421460:glubran; C421461:glubran 2; D011516:Prothrombin; D005340:Fibrinogen