Cyclic AMP-elevating agents induce the expression of MAP kinase phosphatase-1 in PC12 cells.
Stimulation of pheochromocytoma PC12 cells by cAMP-elevating agents caused the induction of the immediate early gene 3CH134, which encodes MAP kinase phosphatase-1 (MKP-1). Forskolin was as potent as serum in stimulating MKP-1 gene expression, whereas dibutyryl-cAMP and neuropeptide PACAP were less effective. Induction of the MKP-1 gene was accompanied by neo-synthesis of MKP-1 protein. MAP kinase activation was not involved in the cAMP-induced MKP-1 gene expression. The MAP kinase inactivation, that would result from MKP-1 induction in response to increased intracellular cAMP level, contributes to explain how hormones or neurotransmitters signaling through cAMP influence cell growth and differentiation.
Burgun
C
C
INSERM U338, Centre de Neurochimie, 5 rue Blaise Pascal, 67084, Strasbourg, France. burgun@neurochem.u-strasbg.fr
Esteve
L
L
Humblot
N
N
Aunis
D
D
Zwiller
J
J
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D018797:Cell Cycle Proteins; D005576:Colforsin / Q000494:pharmacology*; D000242:Cyclic AMP / Q000378:metabolism*; D004268:DNA-Binding Proteins; D054638:Dual Specificity Phosphatase 1; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000502:physiology*; D017781:Genes, Immediate-Early; D017874:Immediate-Early Proteins / Q000235:genetics*; D048031:JNK Mitogen-Activated Protein Kinases; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009479:Neuropeptides / Q000494:pharmacology; D016716:PC12 Cells; D010749:Phosphoprotein Phosphatases; D010766:Phosphorylation; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D054645:Protein Phosphatase 1; D017027:Protein Tyrosine Phosphatases / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D051381:Rats; D014157:Transcription Factors; D050807:ets-Domain Protein Elk-1
C495403:Adcyap1 protein, rat; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; C494114:Elk1 protein, rat; D017874:Immediate-Early Proteins; D009479:Neuropeptides; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D011518:Proto-Oncogene Proteins; D014157:Transcription Factors; D050807:ets-Domain Protein Elk-1; D005576:Colforsin; D003994:Bucladesine; D000242:Cyclic AMP; D048031:JNK Mitogen-Activated Protein Kinases; D020928:Mitogen-Activated Protein Kinases; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1; D054638:Dual Specificity Phosphatase 1; C517021:Dusp1 protein, rat; D017027:Protein Tyrosine Phosphatases