title: Regular disappearance of the human papillomavirus genome after conization of cervical dysplasia by carbon dioxide laser.
abstract: OBJECTIVE
  We wished to evaluate the effectiveness of treatment of cervical dysplasia by laser conization in relation to persistence of human papillomavirus after treatment.
  
  
  METHODS
  Of 203 women referred to colposcopy because of an abnormal Papanicolaou smear, 149 women could be followed up for 3 years. A total of 108 women were treated by carbon dioxide laser excision, 4 women were treated by carbon dioxide laser evaporation, and 37 women were merely followed up. Cervical samples were taken before treatment and at follow-up 3 years later and were analyzed by nested general primer polymerase chain reaction for human papillomavirus deoxyribonucleic acid.
  
  
  RESULTS
  Among women treated by laser conization, 82 (73.2%) had positive results for human papillomavirus deoxyribonucleic acid before treatment. Three women (2.7%) had a positive finding at follow-up, but no woman had the same human papillomavirus type on both occasions. Eighty-eight women had grade 1 to grade 3 cervical intraepithelial neoplasia before treatment, whereas during follow-up only 2 squamous cells atypias were found.
  
  
  CONCLUSIONS
  The human papillomavirus genome present before treatment was regularly cleared, and there was also no recurrence of dysplasia. The results suggest that human papillomavirus testing is useful for monitoring the efficacy of treatment and that treatment modalities resulting in clearance of human papillomavirus should be favored.
authors.affiliation: Department of Obstetrics and Gynecology, Umeâ University Hospital, Umeå, Sweden.
authors.forename: L
authors.initials: L
authors.lastname: Kjellberg
authors.forename: G
authors.initials: G
authors.lastname: Wadell
authors.forename: F
authors.initials: F
authors.lastname: Bergman
authors.forename: M
authors.initials: M
authors.lastname: Isaksson
authors.forename: T
authors.initials: T
authors.lastname: Angström
authors.forename: J
authors.initials: J
authors.lastname: Dillner
mesh_terms: D000328:Adult; D019092:Conization; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D016679:Genome, Viral; D006801:Humans; D053685:Laser Therapy; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics*; D011184:Postoperative Period; D002578:Uterine Cervical Dysplasia / Q000601:surgery* / Q000821:virology* / Q000473:pathology
chemical_list: D004279:DNA, Viral