B-535a, b and c, new sphingosine kinase inhibitors, produced by a marine bacterium; taxonomy, fermentation, isolation, physico-chemical properties and structure determination.
In the course of our screening for inhibitors of sphingosine kinase, we found a series of active compounds in a culture broth of a novel marine bacterium, SANK 71896. The structures of the compounds, named B-5354a, b and c, were elucidated by a combination of spectroscopic analyses to be new esters of 4-amino-3-hydroxybenzoic acid with long-chain unsaturated alcohols. B-5354a, b and c inhibit sphingosine kinase activity with IC50 values of 21, 58 and 38 microm, respectively.
Kono
K
K
Pharmacology and Molecular Biology Research Laboratories, Research Institute, Sankyo Co., Ltd., Shinagawa, Tokyo, Japan.
Tanaka
M
M
Mizuno
T
T
Kodama
K
K
Ogita
T
T
Kohama
T
T
D010129:4-Aminobenzoic Acid / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D002474:Cell-Free System; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D005285:Fermentation; D006090:Gram-Negative Bacteria / Q000145:classification / Q000378:metabolism*; D020128:Inhibitory Concentration 50; D015394:Molecular Structure; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000037:antagonists & inhibitors*; D051381:Rats; D014871:Water Microbiology; D062366:para-Aminobenzoates; D000099157:Sphingosine Kinase
D010129:4-Aminobenzoic Acid; D004791:Enzyme Inhibitors; D017853:Phosphotransferases (Alcohol Group Acceptor); D062366:para-Aminobenzoates; D000099157:Sphingosine Kinase; C416810:B 5354a; C416811:B 5354b; C416812:B 5354c