Developmental expression of the Arabidopsis thaliana CycA2;1 gene.
The associations of cyclins with highly conserved cyclin-dependent kinases are key events in the regulation of cell cycle progression. The spatio-temporal expression of an Arabidopsis thaliana (L.) Heynh. mitotic cyclin, Arath;CycA2;1, was studied by histochemical beta-glucuronidase (GUS) analysis and in-situ hybridizations. The CycA2,1] promoter was active in the egg apparatus before fertilization. During embryogenesis, CycA2;1:gus expression was found in the embryo and the developing endosperm. Throughout plant development, CycA2;1 transcripts were found in both dividing and non-dividing cells, indicating that the expression of this cyclin is not a limiting factor for cell division. In the pericycle and stelar parenchyma, CycA2;1 transcripts were located at the xylem poles, a position that can be correlated with competence for lateral root formation. In addition, CycA2;1:gus expression was upregulated in roots by auxins and in the shoot apex by cytokinins. Transcription of CycA2;1 was shown by reverse transcription-polymerase chain reaction to be strongly induced by sucrose in A. thaliana cell suspensions.
Burssens
S
S
Vakgroep Moleculaire Genetica en Departement Plantengenetics, Vlaams Interuniversitair Instituut voor Biotechnologie, Universiteit Gent, KL, Belgium.
de Almeida Engler
J
J
Beeckman
T
T
Richard
C
C
Shaul
O
O
Ferreira
P
P
Van Montagu
M
M
Inzé
D
D
D017360:Arabidopsis / Q000166:cytology / Q000235:genetics* / Q000254:growth & development; D002453:Cell Cycle / Q000502:physiology*; D002478:Cells, Cultured; D016213:Cyclins / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D005966:Glucuronidase / Q000235:genetics; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012639:Seeds / Q000502:physiology
D016213:Cyclins; D005966:Glucuronidase