title: Antibodies to malaria vaccine candidates Pvs25 and Pvs28 completely block the ability of Plasmodium vivax to infect mosquitoes.
abstract: Transmission-blocking vaccines are one strategy for controlling malaria, whereby sexual-stage parasites are inhibited from infecting mosquitoes by human antibodies. To evaluate whether the recently cloned Plasmodium vivax proteins Pvs25 and Pvs28 are candidates for a transmission-blocking vaccine, the molecules were expressed in yeast as secreted recombinant proteins. Mice vaccinated with these proteins adsorbed to aluminum hydroxide developed strong antibody responses against the immunogens, although for Pvs28, this response was genetically restricted. Antisera against both recombinant Pvs25 and Pvs28 recognized the corresponding molecules expressed by cultured sexual-stage parasites isolated from patients with P. vivax malaria. The development of malaria parasites in mosquitoes was completely inhibited when these antisera were ingested with the infected blood meal. Pvs25 and Pvs28, expressed in Saccharomyces cerevisiae, are as yet the only fully characterized transmission-blocking vaccine candidates against P. vivax that induce such a potent antiparasite response.
authors.affiliation: Malaria Vaccine Development Unit, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland 20852, USA.
authors.forename: H
authors.initials: H
authors.lastname: Hisaeda
authors.forename: A W
authors.initials: AW
authors.lastname: Stowers
authors.forename: T
authors.initials: T
authors.lastname: Tsuboi
authors.forename: W E
authors.initials: WE
authors.lastname: Collins
authors.forename: J S
authors.initials: JS
authors.lastname: Sattabongkot
authors.forename: N
authors.initials: N
authors.lastname: Suwanabun
authors.forename: M
authors.initials: M
authors.lastname: Torii
authors.forename: D C
authors.initials: DC
authors.lastname: Kaslow
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000627:therapeutic use*; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology*; D000954:Antigens, Surface / Q000235:genetics / Q000276:immunology*; D003001:Cloning, Molecular; D009033:Culicidae / Q000469:parasitology*; D007106:Immune Sera / Q000276:immunology; D017780:Malaria Vaccines / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D002679:Pan troglodytes; D010966:Plasmodium vivax / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D007106:Immune Sera; D017780:Malaria Vaccines; C121473:Pvs25 protein, P vivax; C121474:Pvs28 protein, P vivax; D011994:Recombinant Proteins