Degradation of myofibrillar proteins by a calpain-like proteinase in the arm muscle of Octopus vulgaris.
The effects of a calpain-like proteinase (CaDP) isolated from the arm muscle of Octopus vulgaris on the myofibrils and myofibrillar proteins isolated from the same tissue were examined. Our studies clearly showed that treatment of intact myofibrils with CaDP in the presence of 5 mM Ca2+ results in the degradation of the major myofibrillar proteins myosin, paramyosin, and actin. From the isolated alpha- and beta-paramyosins only beta-paramyosin is degraded by CaDP in the presence of 5 mM Ca2+ producing three groups of polypeptides of 80, 75, and 60 kDa, respectively. The degradation rate depends on the proteinase to substrate ratio, temperature, and time of proteolysis and is inhibited by the endogenous CaDP inhibitory factor (CIF), as well as by various known cysteine proteinase inhibitors (E-64, leupeptin, and antipain). From the other myofibrillar proteins examined myosin, but not actin, is degraded by CaDP; myosin heavy chain (MHC, 200 kDa) is degraded by CaDP producing four groups of polypeptides of lower molecular masses (155, 125, 115, and 102 kDa, respectively); the degradation rate depends on the incubation time and the proteinase to substrate ratio. Furthermore, CaDP undergoes limited autolysis in the presence of both the exogenous casein and the endogenous beta-paramyosin producing two large active fragments of 52 and 50.6 kDa, respectively; CIF reversibly inhibits this CaDP autolysis.
Hatzizisis
D
D
Department of Zoology, School of Biology, Aristotle University of Thessaloniki, Greece.
Gaitanaki
C
C
Beis
I
I
D000199:Actins / Q000032:analysis / Q000378:metabolism; D000818:Animals; D001329:Autolysis / Q000378:metabolism; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002154:Calpain / Q000378:metabolism*; D002364:Caseins / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects / Q000502:physiology; D009124:Muscle Proteins / Q000032:analysis / Q000378:metabolism*; D009132:Muscles / Q000737:chemistry / Q000201:enzymology*; D009210:Myofibrils / Q000378:metabolism*; D018995:Myosin Heavy Chains / Q000032:analysis / Q000378:metabolism; D049831:Octopodiformes / Q000378:metabolism*; D013379:Substrate Specificity; D014335:Tropomyosin / Q000032:analysis / Q000378:metabolism
D000199:Actins; D002135:Calcium-Binding Proteins; D002364:Caseins; D009124:Muscle Proteins; D014335:Tropomyosin; C033668:calpastatin; D002154:Calpain; D018995:Myosin Heavy Chains; D002118:Calcium