Conditions for conjugative transposon transfer in Lactococcus lactis.
Three different techniques for bacterial mating were applied to wild type and culture collection strains of Lactococcus lactis harbouring transposons: direct plate conjugation, filter mating and mating on milk agar. Efficiencies and frequencies of transfer were compared. Transconjugants were characterized by marker properties and molecular assays. Transposon-coded Suc+ Nis+ phenotype as well as Suc+ Bac+ Nis- phenotype were transferred with frequencies ranging between 10-9 and 10-6. Milk agar plate mating was the best technique for obtaining gene transfer events involving wild type lactococci.
Blaiotta
G
G
Dipartimento di Scienza degli Alimenti, Università degli Studi di Napoli 'Federico II', Portici, Italy.
Ercolini
D
D
Simeoli
E
E
Moschetti
G
G
Villani
F
F
D003227:Conjugation, Genetic; D003433:Crosses, Genetic; D004251:DNA Transposable Elements; D003611:Dairy Products / Q000382:microbiology; D016521:Electrophoresis, Gel, Pulsed-Field; D005516:Food Microbiology; D013294:Lactococcus lactis / Q000235:genetics*; D010641:Phenotype
D004251:DNA Transposable Elements