Immunocytochemical localization of beta-citryl-L-glutamate in primary neuronal cells and in the differentiation of P19 mouse embryonal carcinoma cells into neuronal cells.
The immunocytochemical localization of beta-citryl-L-glutamate (beta-CG) in primary neuronal cells and in the differentiation of P19 cells was examined. 1: Cells with the morphological features of neurons in the primary culture were specifically stained with the anti-beta-CG antibody both in neurites and in the cell body. 2: The neuronal cells differentiated from P19 cells were distinctly stained with the anti-beta-CG antibody both in neurites and in the cell body, while the non-neuronal cells were not. 3: The concentration of beta-CG was low in the P19 cells, but increased significantly with the differentiation of P19 cells into neurons. It was shown that beta-CG was localized exclusively in neurons. These findings suggest that beta-CG plays functional roles in the differentiation and growth of neuron.
Narahara
M
M
Faculty of Pharmaceutical Sciences, Kobe-Gakuin University, Arise, Ikawadani-cho, Nishi-ku, Kobe 651-2180, Japan. narahara@pharm.kobegakuin.ac.jp
Tachibana
K
K
Adachi
S
S
Iwasa
A
A
Yukii
A
A
Hamada-Kanazawa
M
M
Kawai
Y
Y
Miyake
M
M
D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000166:cytology / Q000187:drug effects / Q000196:embryology; D018236:Carcinoma, Embryonal / Q000473:pathology* / Q000648:ultrastructure; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects / Q000196:embryology; D002642:Chick Embryo; D005971:Glutamates / Q000378:metabolism*; D007150:Immunohistochemistry; D051379:Mice; D009474:Neurons / Q000378:metabolism* / Q000648:ultrastructure; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D005971:Glutamates; D014212:Tretinoin; C018491:beta-citrylglutamic acid