title: Evaluation of p-naphtholbenzein-beta-D-galactoside as a substrate for bacterial beta-galactosidase.
abstract: We describe the synthesis of a new substrate for the detection of beta-galactosidase and evaluate its performance in comparison with 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) and cyclohexenoesculetinbeta-D-galactoside (CHE-Gal). Of 206 Enterobacteriaceae strains able to hydrolyze X-Gal, 194 (94.2%) hydrolyzed CHE-Gal and 192 (93.2%) hydrolyzed p-naphtholbenzein-beta-D-galactoside (PNB-Gal). We conclude that PNB-Gal is an effective substrate for the detection of beta-galactosidase.
authors.affiliation: Department of Chemical & Life Sciences, University of Northumbria, Newcastle-upon-Tyne, United Kingdom.
authors.forename: A L
authors.initials: AL
authors.lastname: James
authors.forename: K F
authors.initials: KF
authors.lastname: Chilvers
authors.forename: J D
authors.initials: JD
authors.lastname: Perry
authors.forename: L
authors.initials: L
authors.lastname: Armstrong
authors.forename: F K
authors.initials: FK
authors.lastname: Gould
mesh_terms: D002863:Chromogenic Compounds / Q000138:chemical synthesis; D004755:Enterobacteriaceae / Q000201:enzymology*; D005697:Galactosides / Q000138:chemical synthesis; D007211:Indoles; D009281:Naphthalenes / Q000138:chemical synthesis; D013379:Substrate Specificity; D014468:Umbelliferones; D015008:Yersinia enterocolitica / Q000201:enzymology; D001616:beta-Galactosidase / Q000378:metabolism*
chemical_list: D002863:Chromogenic Compounds; D005697:Galactosides; D007211:Indoles; D009281:Naphthalenes; D014468:Umbelliferones; C101993:cyclohexenoesculetin-beta-galactoside; C419824:p-naphtholbenzeingalactoside; D001616:beta-Galactosidase; C044888:5-bromo-4-chloro-3-indolyl beta-galactoside