title: Enzymatic saccharification of solid residue of olive mill in a batch reactor.
abstract: This paper describes the enzymatic hydrolysis of solid residue of olive mill (OMRS) in a batch reactor with the Trichoderma reesei enzyme. Before enzymatic saccharification, crude lignocellulosic material is submitted to alkaline pre-treatment with NaOH. Optimum conditions of the pre-treatment (temperature of T=100 degrees C and OMRS-NaOH concentration ratio of about R=20) were determined. The optimum enzymatic conditions determined were as follows: pH of about 5, temperature of T=50 degrees C and enzyme to mass substrate mass ratio E/S=0.1g enzyme (g OMRS)(-1). The maximum saccharification yield obtained at optimum experimental conditions was about 50%. The experimental results agree with Lineweaver Burk's formula for low substrate concentrations. At substrate concentrations greater than 40gdm(-3), inhibitory effects were encountered. The kinetic constants obtained for the batch reactor were K(m)=0.1gdm(-3)min(-1) and V(m)=800gdm(-3).
authors.affiliation: Ecole Nationale Polytechnique d'Alger, 10 avenue Pasteur, B.P. 182, El Harrach, Alger, Algeria
authors.initials: N
authors.lastname: Abdi
authors.initials: F
authors.lastname: Hamdache
authors.initials: D
authors.lastname: Belhocine
authors.initials: H
authors.lastname: Grib
authors.initials: H
authors.lastname: Lounici
authors.initials: DL
authors.lastname: Piron
authors.initials: N
authors.lastname: Mameri