The role of perisynaptic Schwann cells in development of neuromuscular junctions in the frog (Xenopus laevis).
Fluorescence microscopy was used to study the behavior of perisynaptic Schwann cells (PSCs) in relation to motor nerve terminals and postsynaptic clusters of acetylcholine receptors, during the development of the neuromuscular junction (NMJ) in the frog Xenopus laevis. Pectoral (supracoracoideus) muscles were labeled with monoclonal antibody 2A12 for Schwann cells, the dye FM4-64 for nerve terminals (NTs), alpha-bungarotoxin for acetylcholine receptors (AChRs), and Hoechst 33258 for cellular nuclei, in animals from tadpole stage 57 to fully grown adults. When muscle fibers first appeared in stage 57, NMJs consisted of tightly apposed NTs and AChRs and were only partially covered with PSCs or their processes. Within a few stages, PSCs fully occupied and overgrew the NMJs, extending fine sprouts between a few micrometers and hundreds of micrometers beyond the borders of the junction. Sprouts of PSCs were most abundant during the time when secondary myogenesis, synaptogenesis, and synaptic growth occurred at their highest rates. PSCs were recruited to NMJs during synaptic growth, at rates between 1.3 PSCs/100 microm junctional length early on and 0.4 PSCs/100 microm later. Shortly after metamorphosis, PSC sprouts disappeared and NMJs acquired the adult appearance, in which PSCs, NTs, and AChRs were mostly congruent. The results suggest that, although PSCs may not be required for initial nerve-muscle contacts, PSCs sprouts lead synaptic growth and play a role in the extension and maturation of developing NMJs.
Herrera
A A
AA
Neurobiology Section, Department of Biological Sciences, University of Southern California, Los Angeles, California 90089-2520, USA. aherrera@usc.edu
Qiang
H
H
Ko
C P
CP
D000367:Age Factors; D000818:Animals; D000906:Antibodies; D002038:Bungarotoxins; D002452:Cell Count / Q000706:statistics & numerical data; D048429:Cell Size / Q000502:physiology; D005456:Fluorescent Dyes; D007814:Larva / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D024510:Muscle Development; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000378:metabolism; D018482:Muscle, Skeletal / Q000166:cytology / Q000254:growth & development / Q000294:innervation; D009469:Neuromuscular Junction / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D009580:Nitroblue Tetrazolium; D017729:Presynaptic Terminals / Q000378:metabolism* / Q000648:ultrastructure; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds; D011950:Receptors, Cholinergic / Q000378:metabolism*; D012583:Schwann Cells / Q000166:cytology / Q000378:metabolism*; D013570:Synaptic Membranes / Q000378:metabolism / Q000648:ultrastructure; D014982:Xenopus laevis / Q000033:anatomy & histology / Q000254:growth & development* / Q000378:metabolism
D000906:Antibodies; D002038:Bungarotoxins; C092350:FM 4-64; D005456:Fluorescent Dyes; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds; D011950:Receptors, Cholinergic; D009580:Nitroblue Tetrazolium