title: Benzimidazole resistance in equine cyathostomes in Slovakia.
abstract: The present study included 19 stud farms, including 243 horses, that were investigated for the occurrence of anthelmintic resistant cyathostomes. The number of horses on the farms varied from nine to more than 100, and horses of all ages were included. A minimum of seven horses were used for faecal egg count reduction (FECR) tests. The anthelmintics included were: fenbendazole (paste formulation), ivermectin (paste formulation) and pyrantel (powder). Resistance to benzimidazoles was detected on 14 farms, with FECR values ranging from 65.1 to 86.3%. Larval cultures after fenbendazole treatment revealed exclusively cyathostome larvae. Ivermectin was tested on eight farms and proved to be effective on all. Pyrantel was tested on two farms and FECR test indicated high efficacy (92-97%). Egg hatch assay (EHA) results showed that mean concentrations of thiabendazole that inhibited hatching in 50% of the eggs (ED(50)) in resistant populations were over 0.1 microg ml(-1). The results of our study suggest widespread resistance to fenbendazole in equine cyathostomes in Slovakia, and possible strategies to delay anthelmintic resistance are discussed briefly.
authors.affiliation: Parasitological Institute, Slovak Academy of Sciences, Hlinkova 3, 040 01, Kosice, Slovak Republic. varady@saske.sk
authors.forename: M
authors.initials: M
authors.lastname: Várady
authors.forename: A
authors.initials: A
authors.lastname: Königová
authors.forename: J
authors.initials: J
authors.lastname: Corba
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D001562:Benzimidazoles / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D004351:Drug Resistance; D005243:Feces / Q000469:parasitology; D005273:Fenbendazole / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D006734:Horse Diseases / Q000188:drug therapy / Q000469:parasitology*; D006736:Horses; D007559:Ivermectin / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D016015:Logistic Models; D010270:Parasite Egg Count / Q000662:veterinary; D011715:Pyrantel / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D018154:Slovakia; D017206:Strongylida Infections / Q000188:drug therapy / Q000469:parasitology / Q000662:veterinary*; D013320:Strongyloidea / Q000187:drug effects*
chemical_list: D000871:Anthelmintics; D001562:Benzimidazoles; D011715:Pyrantel; D005273:Fenbendazole; D007559:Ivermectin