A distinct glycoform of CD44 is an L-selectin ligand on human hematopoietic cells.
We previously have obtained operational evidence of a hematopoietic cell L-selectin ligand expressed on normal human hematopoietic cells and on leukemic blasts. Using a technique developed in our laboratory for analyzing and identifying adhesion molecules, we show here that hematopoietic cell L-selectin ligand is a specialized glycoform of CD44. This L-selectin ligand activity of CD44 requires sialofucosylated N-linked glycans and is sulfation-independent. These data provide important insights on the structural biology of CD44 and reveal a role for this protein as an L-selectin ligand on human hematopoietic cells.
Dimitroff
C J
CJ
Department of Medicine and Dermatology, Brigham and Women's Hospital and Harvard Skin Disease Research Center, Harvard Medical School, Massachusetts General Hospital, Boston, MA 02115, USA.
Lee
J Y
JY
Fuhlbrigge
R C
RC
Sackstein
R
R
D015153:Blotting, Western; D001854:Bone Marrow Cells / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D018960:Hyaluronan Receptors / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D019041:L-Selectin / Q000378:metabolism*; D008024:Ligands; D011134:Polysaccharides / Q000737:chemistry; D011487:Protein Conformation
D018960:Hyaluronan Receptors; D008024:Ligands; D011134:Polysaccharides; D019041:L-Selectin