Determination of nociceptin-orphanin FQ metabolites by capillary LC-MS.
Nociceptin-orphanin FQ (OFQ/N) is a newly discovered peptide involved in pain transmission. The method is described to identify metabolic pathway of this neuropeptide in the spinal cord of rats using capillary size-exclusion liquid chromatography coupled to electrospray ionization mass spectrometry. The applied technique is rapid and selective, and allows for simultaneous measurement and quantitation of several fragments in the incubation mixture.
Suder
P
P
Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060, Krakow, Poland.
Kotlińska
J
J
Legowska
A
A
Smoluch
M
M
Höhne
G
G
Chervet
J P
JP
Rolka
K
K
Silberring
J
J
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D008297:Male; D018847:Opioid Peptides / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D021241:Spectrometry, Mass, Electrospray Ionization; D013116:Spinal Cord / Q000378:metabolism*; D000097629:Nociceptin
D018847:Opioid Peptides; D000097629:Nociceptin