title: The different behaviors of skeletal muscle cells and chondrocytes on PEGT/PBT block copolymers are related to the surface properties of the substrate.
abstract: The attachment, proliferation, morphology, and differentiation of two cell types-skeletal muscle cells and chondrocytes-were investigated on different compositions of poly(ethylene glycol) and poly(butylene terephthalate) segmented block copolymers. Four weight percentages (40, 55, 60, and 70%) and two different molecular weights (300 and 1000 Da) of poly(ethylene glycol) were tested. Varying the weight percentage and molecular weight of poly(ethylene glycol) resulted in different behaviors for skeletal muscle cells and chondrocytes. The attachment of skeletal muscle was the highest (similar to tissue culture polystyrene) when copolymers containing 55 wt % of poly(ethylene glycol) were used, regardless of the poly(ethylene glycol) molecular weight. Maximum proliferation and differentiation of skeletal muscle cells was achieved when copolymers containing 55 wt % and 300 Da molecular weight of poly(ethylene glycol) were used. In contrast, the weight percentage and molecular weight of poly(ethylene glycol) had no significant effect on chondrocyte attachment and proliferation; the attached chondrocytes retained a differentiated phenotype only when a 70 wt % of poly(ethylene glycol) was used. Cell behavior was correlated with the surface properties of the copolymer films, as indicated by contact-angle measurements. These results suggest that an optimized wt % and molecular weight of poly(ethylene glycol) will be useful depending on the specific cell type.
authors.affiliation: Division of Health Sciences & Technology and Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
authors.forename: M
authors.initials: M
authors.lastname: Papadaki
authors.forename: T
authors.initials: T
authors.lastname: Mahmood
authors.forename: P
authors.initials: P
authors.lastname: Gupta
authors.forename: M B
authors.initials: MB
authors.lastname: Claase
authors.forename: D W
authors.initials: DW
authors.lastname: Grijpma
authors.forename: J
authors.initials: J
authors.lastname: Riesle
authors.forename: C A
authors.initials: CA
authors.lastname: van Blitterswijk
authors.forename: R
authors.initials: R
authors.lastname: Langer
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D015153:Blotting, Western; D002417:Cattle; D002448:Cell Adhesion; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D002469:Cell Separation; D019902:Chondrocytes / Q000502:physiology* / Q000648:ultrastructure; D003584:Cytological Techniques; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D018482:Muscle, Skeletal / Q000166:cytology* / Q000648:ultrastructure; D011091:Polyesters; D011092:Polyethylene Glycols; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; C072932:polyethylene oxide-polybutylene terephthalate copolymer; D011092:Polyethylene Glycols