title: Requirement for the lpA1 lysophosphatidic acid receptor gene in normal suckling behavior.
abstract: Although extracellular application of lysophosphatidic acid (LPA) has been extensively documented to produce a variety of cellular responses through a family of specific G protein-coupled receptors, the in vivo organismal role of LPA signaling remains largely unknown. The first identified LPA receptor gene, lp(A1)/vzg-1/edg-2, was previously shown to have remarkably enriched embryonic expression in the cerebral cortex and dorsal olfactory bulb and postnatal expression in myelinating glia including Schwann cells. Here, we show that targeted deletion of lp(A1) results in approximately 50% neonatal lethality, impaired suckling in neonatal pups, and loss of LPA responsivity in embryonic cerebral cortical neuroblasts with survivors showing reduced size, craniofacial dysmorphism, and increased apoptosis in sciatic nerve Schwann cells. The suckling defect was responsible for the death among lp(A1)((-/-)) neonates and the stunted growth of survivors. Impaired suckling behavior was attributable to defective olfaction, which is likely related to developmental abnormalities in olfactory bulb and/or cerebral cortex. Our results provide evidence that endogenous lysophospholipid signaling requires an lp receptor gene and indicate that LPA signaling through the LP(A1) receptor is required for normal development of an inborn, neonatal behavior.
authors.affiliation: Department of Pharmacology, Neuroscience Graduate Program, Biomedical Sciences Program, School of Medicine, University of California, San Diego, La Jolla, CA 92093-0636, USA.
authors.forename: J J
authors.initials: JJ
authors.lastname: Contos
authors.forename: N
authors.initials: N
authors.lastname: Fukushima
authors.forename: J A
authors.initials: JA
authors.lastname: Weiner
authors.forename: D
authors.initials: D
authors.lastname: Kaushal
authors.forename: J
authors.initials: J
authors.lastname: Chun
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000833:Animals, Suckling; D002540:Cerebral Cortex / Q000196:embryology / Q000502:physiology; D019465:Craniofacial Abnormalities / Q000235:genetics; D003433:Crosses, Genetic; D005260:Female; D005313:Fetal Death; D017353:Gene Deletion; D005838:Genotype; D006130:Growth Disorders / Q000235:genetics; D008246:Lysophospholipids / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D009474:Neurons / Q000166:cytology; D009924:Organ Culture Techniques; D011956:Receptors, Cell Surface / Q000172:deficiency / Q000235:genetics* / Q000502:physiology; D043562:Receptors, G-Protein-Coupled; D049368:Receptors, Lysophosphatidic Acid; D011995:Recombination, Genetic; D013391:Sucking Behavior / Q000502:physiology*
chemical_list: D008246:Lysophospholipids; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D049368:Receptors, Lysophosphatidic Acid