Escherichia coli strain with a deletion of the chromosomal ampC gene marked with TcR, suitable for production of penicillin G acylase.
Escherichia coli strain which contains a marker of tetracycline resistance gene (TcR) placed by P1 transduction beside the chromosomal deletion of ampC gene (delta ampC) coding for beta-lactamase was constructed. Such introduction of TcR marker permits a fast and simple selection for the transfer of delta ampC by P1 transduction into industrial E. coli strains. This approach was used for constructing an E. coli strain suitable for penicillin acylase production.
Vizváryová
M
M
Department of Molecular Biology, Comenius University, Bratislava, Slovakia.
Stuchlík
S
S
Grones
J
J
Macor
M
M
Turna
J
J
D001426:Bacterial Proteins; D017101:Bacteriophage P1 / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D017353:Gene Deletion; D005798:Genes, Bacterial; D005819:Genetic Markers; D010399:Penicillin Amidase / Q000096:biosynthesis* / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D013753:Tetracycline Resistance / Q000235:genetics; D014161:Transduction, Genetic; D001618:beta-Lactamases / Q000235:genetics
D001426:Bacterial Proteins; D005819:Genetic Markers; D011994:Recombinant Proteins; D010399:Penicillin Amidase; C048795:AmpC beta-lactamases; D001618:beta-Lactamases