In vitro selection of a ligase ribozyme carrying alkylamino groups in the side chains.
A novel ligase ribozyme was in vitro selected from a random-sequence RNA library including N(6)-aminohexyl-modified adenine residues in place of natural adenine residues. This ribozyme mediated the formation of a phosphodiester bond with a DNA oligonucleotide through condensation with a 5'-triphosphate moiety on the ribozyme. Among the clones isolated from this selection, one was shown to accelerate ligation about 250-fold compared to the original random-sequence RNA library. Almost no rate acceleration was observed when the N(6)-aminohexyl-groups on adenine residues were omitted. Furthermore, ligation was dependent on the presence of a template DNA oligonucleotide that bridged the two strands.
Teramoto
N
N
Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Kyoto, 606-8501, Japan.
Imanishi
Y
Y
Ito
Y
Y
D001483:Base Sequence; D002384:Catalysis; D003001:Cloning, Molecular; D008025:Ligases / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides; D016337:RNA, Catalytic / Q000737:chemistry* / Q000378:metabolism
D009838:Oligodeoxyribonucleotides; D016337:RNA, Catalytic; D008025:Ligases