Cloning, expression and functional characterisation of a peroxiredoxin from the potato cyst nematode Globodera rostochiensis.
We report the cloning, expression and functional characterisation of a peroxidase belonging to the peroxiredoxin family from the potato cyst nematode Globodera rostochiensis, the first molecule of this type from any nematode parasitic on plants. The G. rostochiensis peroxiredoxin catalyses the breakdown of hydrogen peroxide, but not cumene or t-butyl hydroperoxide, in a trypanosomatid reducing system comprising trypanothione reductase, trypanothione and tryparedoxin. In common with its homologues from Onchocerca volvulus and Brugia malayi, the G. rostochiensis enzyme is present on the surface of invasive and post-infective juveniles despite the apparent lack of a cleavable N-terminal signal peptide. The possibility that the G. rostochiensis peroxiredoxin plays a role in protection of the parasite from plant defence responses is discussed.
Robertson
L
L
Department of Nematology, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, Scotland, UK. lee.robertson@uam.es
Robertson
W M
WM
Sobczak
M
M
Helder
J
J
Tetaud
E
E
Ariyanayagam
M R
MR
Ferguson
M A
MA
Fairlamb
A
A
Jones
J T
JT
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D003001:Cloning, Molecular; D018509:DNA, Helminth / Q000235:genetics; D015723:Gene Library; D006861:Hydrogen Peroxide / Q000378:metabolism; D018551:Solanum lycopersicum / Q000469:parasitology; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D054464:Peroxiredoxins; D018517:Plant Roots / Q000469:parasitology; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D011198:Solanum tuberosum / Q000469:parasitology; D013379:Substrate Specificity; D014431:Tylenchoidea / Q000235:genetics* / Q000502:physiology
D018509:DNA, Helminth; D011994:Recombinant Proteins; D006861:Hydrogen Peroxide; D010544:Peroxidases; D054464:Peroxiredoxins