title: Effect of low-dose citrate anticoagulation on the clinical safety and efficacy of direct adsorption of lipoproteins (DALI apheresis) in hypercholesterolemic patients: a prospective controlled clinical trial.
abstract: Direct adsorption of lipoproteins (DALI) is the first lipid apheresis system compatible with whole blood with the advantage of a very simple procedure. A mixture of heparin plus citrate (ACD-A) is used for the anticoagulation regimen (AR). A clinical, prospective, controlled crossover study was performed to test the safety and efficacy of low-dose citrate (LDC) anticoagulation in DALI. Five chronic DALI patients suffering from coronary heart disease and hypercholesterolemia underwent 3 DALI sessions each using the LDC anticoagulation regimen (60 IU heparin/kg body weight as initial bolus; 1:40 ACD-A: blood as perfusion). This was compared to 3 sessions per patient with the standard AR (bolus of 20 IU heparin/kg, 1:20 ACD-A as perfusion). Patient blood volumes (1.6; average of 7,040 ml) were treated with 750 ml adsorber gel per session at a blood flow rate of 60 ml/min. Mean LDL and Lp(a) reductions exceeded 60% with both AR. No clinical side effects were observed. Both AR controlled the coagulation well as evidenced by a sufficient prolongation of the partial prothrombin time (PTT) and activated clotting time as well as low thrombin-antithrombin (TAT) formation. Biocompatibility parameters exhibited favorable results (low activation of complement and cells, and only slight formation of C3a, C5a, beta-thromboglobulin, elastase, and TNF-alpha). The asymptomatic bradykinin generation was comparable in both study arms. LDC optimized the ionized calcium levels and pH in the efferent blood postadsorber. LDC anticoagulation was safe and effective, and may further improve the tolerance of DALI apheresis in hypercholesterolemic patients.
authors.affiliation: Nephrology Division, Medical Clinic I, Klinikum Grosshadern, University of Munich, Germany. bosch@med1.med.uni-muenchen.de
authors.forename: T
authors.initials: T
authors.lastname: Bosch
authors.forename: O
authors.initials: O
authors.lastname: Heinemann
authors.forename: C
authors.initials: C
authors.lastname: Duhr
authors.forename: T
authors.initials: T
authors.lastname: Wendler
authors.forename: C
authors.initials: C
authors.lastname: Keller
authors.forename: E
authors.initials: E
authors.lastname: Fink
authors.forename: T
authors.initials: T
authors.lastname: Kirschner
authors.forename: S
authors.initials: S
authors.lastname: Klebert
authors.forename: W
authors.initials: W
authors.lastname: Samtleben
mesh_terms: D000327:Adsorption; D000368:Aged; D000925:Anticoagulants / Q000008:administration & dosage*; D001772:Blood Cell Count; D001774:Blood Chemical Analysis; D001781:Blood Component Removal / Q000379:methods*; D001784:Blood Gas Analysis; D019343:Citric Acid / Q000008:administration & dosage*; D018592:Cross-Over Studies; D005260:Female; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood / Q000628:therapy*; D008077:Lipoproteins, LDL / Q000097:blood*; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D018709:Statistics, Nonparametric
chemical_list: D000925:Anticoagulants; D008077:Lipoproteins, LDL; D019343:Citric Acid