Expression of cyclooxygenase-2 and nitrotyrosine in human gastric mucosa before and after Helicobacter pylori eradication.
To determine whether cure of Helicobacter pylori infection influences the expression of COX-2 and nitrotyrosine in the distal stomach of humans, biopsy specimens were examined immunohistochemically. H. pylori infection was determined using a rapid urease test, culture and histology. Positive staining of COX-2/nitrotyrosine in the epithelium was expressed as the percentage of stained cells to the total epithelial cells. There was a significant increase in COX-2/nitrotyrosine staining in H. pylori -positive subjects compared with H. pylori -negative subjects. Cure of the infection resulted in a significant decrease in both COX-2/nitrotyrosine staining in all patients (52.1+/-12.1% vs 15. 4+/-7.2%, P<0.001; and 57.3+/-13.6% vs 36.1+/-18.0%, P<0.01, respectively). However, immunoreactivity of COX-2/nitrotyrosine was observed in all cases with intestinal metaplasia even after the cure of H. pylori infection.Thus, cure of H. pylori infection may decrease the risk of gastric carcinogenesis due to COX-2 and NO-related compounds in gastric mucosa but not in those patients with intestinal metaplasia.
Kimura
A
A
Department of Internal Medicine and Therapeutics, Osaka University Graduate School of Medicine, Suita, Japan.
Tsuji
S
S
Tsujii
M
M
Sawaoka
H
H
Iijima
H
H
Kawai
N
N
Yasumaru
M
M
Kakiuchi
Y
Y
Okuda
Y
Y
Ali
Z
Z
Nishimura
Y
Y
Sasaki
Y
Y
Kawano
S
S
Hori
M
M
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D051546:Cyclooxygenase 2; D004724:Endoscopy; D005753:Gastric Mucosa / Q000378:metabolism* / Q000382:microbiology*; D005756:Gastritis / Q000378:metabolism / Q000382:microbiology; D016480:Helicobacter pylori / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000096:biosynthesis*; D008565:Membrane Proteins; D008875:Middle Aged; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000096:biosynthesis*; D014456:Ulcer / Q000378:metabolism / Q000382:microbiology; D014510:Urease / Q000378:metabolism
D007527:Isoenzymes; D008565:Membrane Proteins; C002744:3-nitrotyrosine; D014443:Tyrosine; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases; D014510:Urease