title: Effect of calcium in assay medium on D value of Bacillus stearothermophilus ATCC 7953 spores.
abstract: The D value of commercial biological indicator spore strips using Bacillus stearothermophilus ATCC 7953 was increased by higher calcium concentrations in assay media. The calcium concentration in assay media varied among the manufacturers. The calcium concentration in assay media is an important factor to consider to minimize the variation of D value.
authors.affiliation: Quality Control Department, Misato Plant, Eisai Co., Ltd., Misato-Machi, Kodama-Gun, Saitama 367-0198, Japan. k-sasaki@hhc.eisai.co.jp
authors.forename: K
authors.initials: K
authors.lastname: Sasaki
authors.forename: H
authors.initials: H
authors.lastname: Shintani
authors.forename: J
authors.initials: J
authors.lastname: Itoh
authors.forename: T
authors.initials: T
authors.lastname: Kamogawa
authors.forename: Y
authors.initials: Y
authors.lastname: Kajihara
mesh_terms: D001431:Bacteriological Techniques; D002118:Calcium / Q000032:analysis / Q000494:pharmacology*; D003470:Culture Media / Q000737:chemistry; D001411:Geobacillus stearothermophilus / Q000187:drug effects* / Q000302:isolation & purification* / Q000502:physiology; D013171:Spores, Bacterial / Q000187:drug effects* / Q000302:isolation & purification*; D013242:Sterilization
chemical_list: D003470:Culture Media; D002118:Calcium