title: Mechanism for the peroxynitrite scavenging activity by anthocyanins.
abstract: We show that anthocyanins can function as potent inhibitors of the formation of nitrated tyrosine in vitro, and clarified how pelargonidin (Pel), which has a mono-hydroxyl group on the B-ring, can scavenge peroxynitrite (ONOO(-)) by detection of the reaction products. Pel was reacted with ONOO(-), then the reaction mixture was analyzed using high-performance liquid chromatography (HPLC). The HPLC analyses showed two novel peaks assumed to be the reaction products. Based on the instrumental analyses, the reaction products were identified as p-hydroxybenzoic acid and 4-hydroxy-3-nitrobenzoic acid. Pel can protect tyrosine from undergoing nitration through the formation of p-hydroxybenzoic acid and 4-hydroxy-3-nitrobenzoic acid.
authors.affiliation: Tokaigakuen Women's College, Nagoya, Japan. tsudata@tokaigakuen-c.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Tsuda
authors.forename: Y
authors.initials: Y
authors.lastname: Kato
authors.forename: T
authors.initials: T
authors.lastname: Osawa
mesh_terms: D000872:Anthocyanins / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D016166:Free Radical Scavengers; D007700:Kinetics; D009566:Nitrates / Q000737:chemistry*; D009579:Nitrobenzoates / Q000032:analysis; D016877:Oxidants / Q000737:chemistry*; D010226:Parabens / Q000032:analysis; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D000872:Anthocyanins; D016166:Free Radical Scavengers; D009566:Nitrates; D009579:Nitrobenzoates; D016877:Oxidants; D010226:Parabens; C039642:peroxynitric acid; C002744:3-nitrotyrosine; D014443:Tyrosine; C038193:4-hydroxybenzoic acid