title: The crystal structure of UUCG tetraloop.
abstract: All large structured RNAs contain hairpin motifs made of a stem closed by several looped nucleotides. The most frequent loop motif is the UUCG one. This motif belongs to the tetraloop family and has the peculiarity of being highly thermodynamically stable. Here, we report the first crystal structure of two UUCG tetraloops embedded in a larger RNA-protein complex solved at 2.8 A resolution. The two loops present in the asymmetric unit are in a different crystal packing environment but, nevertheless, have an identical conformation. The observed structure is globally close to that obtained in solution by nuclear magnetic resonance. However, subtle differences point to a more detailed picture of the role played by 2'-hydroxyl groups in stabilising this tetraloop.
authors.affiliation: UPR 9002 du CNRS, IBMC, 15 rue R. Descartes, 67084 Strasbourg-cedex, France.
authors.forename: E
authors.initials: E
authors.lastname: Ennifar
authors.forename: A
authors.initials: A
authors.lastname: Nikulin
authors.forename: S
authors.initials: S
authors.lastname: Tishchenko
authors.forename: A
authors.initials: A
authors.lastname: Serganov
authors.forename: N
authors.initials: N
authors.lastname: Nevskaya
authors.forename: M
authors.initials: M
authors.lastname: Garber
authors.forename: B
authors.initials: B
authors.lastname: Ehresmann
authors.forename: C
authors.initials: C
authors.lastname: Ehresmann
authors.forename: S
authors.initials: S
authors.lastname: Nikonov
authors.forename: P
authors.initials: P
authors.lastname: Dumas
mesh_terms: D001483:Base Sequence; D018360:Crystallography, X-Ray; D006860:Hydrogen Bonding; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009038:Motion; D019906:Nuclear Magnetic Resonance, Biomolecular; D009690:Nucleic Acid Conformation; D020871:RNA Stability; D012336:RNA, Ribosomal, 16S / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000737:chemistry / Q000378:metabolism*; D012997:Solvents; D013816:Thermodynamics
chemical_list: D012336:RNA, Ribosomal, 16S; D012269:Ribosomal Proteins; D012997:Solvents; C036004:ribosomal protein S15