Electrochemical assay of endo-depolymerase activity of cellulases.
A method for determination of endo-1,4-beta-D-glucanase activity of cellulase samples based on the indirect measurement of decrease in viscosity of a carboxymethylcellulose solution in an electrochemical cell in the presence of an electron carrier was developed. A rotating disk electrode is used as the working electrode. When two reactions (enzymatic and electrochemical) proceeded in the cell simultaneously, the limiting diffusion current at a constant applied potential increases as the viscosity of the solution decreases. Conditions where the initial rate of change of diffusion current (dI/dt) is proportional to the enzyme concentration were found. A good correlation between the new method and a previously known viscometric method for determination of endoglucanase activity was observed.
Skomarovsky
A A
AA
School of Chemistry, Lomonosov Moscow State University, Moscow, 119899, Russia. askomarovsky@enzyme.chem.msu.ru
Zorov
I N
IN
Grishutin
S G
SG
Markov
A V
AV
Sinitsyn
A P
AP
Gusakov
A V
AV
Ryabov
A D
AD
Gnedenko
B B
BB
D002266:Carboxymethylcellulose Sodium; D002480:Cellulase / Q000032:analysis / Q000378:metabolism* / Q000592:standards; D004563:Electrochemistry / Q000295:instrumentation; D012015:Reference Standards; D014783:Viscosity
D002480:Cellulase; D002266:Carboxymethylcellulose Sodium