Opposite role of alpha2- and beta-adrenoceptors in the modulation of interleukin-10 production in endotoxaemic mice.
Our aim was to investigate the role of adrenoceptors in the modulation of in vivo interleukin-10 (IL-10) production in lipopolysaccharide (LPS)-treated mice. The effect of different adrenergic drugs on plasma concentration of IL-10 was measured by ELISA 90 min after LPS injection. Our results confirmed the involvement of beta-adrenoceptors since the beta-agonist isoproterenol significantly increased the IL-10 production in response to LPS stimulation, whereas the beta-antagonists propranolol decreased it. In contrast, the alpha2-agonists UK-14304, clonidine and xylazine significantly decreased the IL-10 plasma level, whereas the alpha2-antagonists CH-38083, prazosine and WB-4101 increased it. Our results provide the first in vivo evidence that, in addition to beta-adrenoceptors; alpha-adrenoceptors play also a very important role in the regulation of IL-10 production under endotoxaemic conditions.
Szelényi
J
J
Department of Pharmacology, Institute of Experimental Medicine, Budapest, Hungary.
Kiss
J P
JP
Puskás
E
E
Selmeczy
Z
Z
Szelényi
M
M
Vizi
E S
ES
D000316:Adrenergic alpha-Agonists / Q000494:pharmacology; D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology; D000818:Animals; D019446:Endotoxemia / Q000097:blood / Q000276:immunology / Q000503:physiopathology*; D004797:Enzyme-Linked Immunosorbent Assay; D016753:Interleukin-10 / Q000096:biosynthesis / Q000097:blood*; D007545:Isoproterenol / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000633:toxicity*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011433:Propranolol / Q000494:pharmacology*; D018341:Receptors, Adrenergic, alpha-2 / Q000502:physiology*; D011943:Receptors, Adrenergic, beta / Q000502:physiology*; D013997:Time Factors
D000316:Adrenergic alpha-Agonists; D000317:Adrenergic alpha-Antagonists; D008070:Lipopolysaccharides; D018341:Receptors, Adrenergic, alpha-2; D011943:Receptors, Adrenergic, beta; D016753:Interleukin-10; D011433:Propranolol; D007545:Isoproterenol