title: Use of molecular beacons to detect an antifolate resistance-associated mutation in Plasmodium falciparum.
abstract: A PCR-based technique using new fluorescent probes, called molecular beacons, was developed to detect the antifolate resistance-associated S108N point mutation in Plasmodium falciparum. One hundred African clinical isolates were tested by the new method in comparison with the PCR-restriction fragment length polymorphism method. This new molecular technique appears to be a promising tool for epidemiological studies.
authors.affiliation: Centre National de Référence pour la Chimiosensibilité du Paludisme, Université Paris V et Assistance Publique-Hôpitaux de Paris, Hôpital Bichat-Claude Bernard, Laboratoire de Parasitologie, Paris, France. rjdurand@club-internet.fr
authors.forename: R
authors.initials: R
authors.lastname: Durand
authors.forename: J
authors.initials: J
authors.lastname: Eslahpazire
authors.forename: S
authors.initials: S
authors.lastname: Jafari
authors.forename: J F
authors.initials: JF
authors.lastname: Delabre
authors.forename: A
authors.initials: A
authors.lastname: Marmorat-Khuong
authors.forename: J P
authors.initials: JP
authors.lastname: di Piazza
authors.forename: J
authors.initials: J
authors.lastname: Le Bras
mesh_terms: D000818:Animals; D004351:Drug Resistance / Q000235:genetics; D005493:Folic Acid Antagonists / Q000494:pharmacology*; D006801:Humans; D009154:Mutation; D010963:Plasmodium falciparum / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*
chemical_list: D005493:Folic Acid Antagonists; D013762:Tetrahydrofolate Dehydrogenase