Preliminary report on a method of human papillomavirus DNA extraction from verruca plantaris for polymerase chain reaction studies.
This preliminary report on the Chelex-based extraction method of HPV DNA from verruca plantaris is the first step in a series of studies on verruca plantaris undertaken, jointly, by the Departments of Podiatric Medicine and Microbiology at Des Moines University-Osteopathic Medical Center, Des Moines, Iowa. Current projects include the development of new primers for PCR studies of HPV, exploration of different viral DNA extraction methods, and surveys concerning treatment, success of therapies, and the epidemiology of verruca plantaris.
Pfannenstein
R R
RR
Department of Podiatric Medicine, College of Podiatric Medicine, Des Moines University-Osteopathic Medical Center, Iowa, USA.
Johnson
J C
JC
Bennett
J D
JD
Evans
R D
RD
John
K J
KJ
Hayes
D W
DW
D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D005534:Foot Diseases / Q000145:classification / Q000235:genetics* / Q000628:therapy; D005838:Genotype; D006801:Humans; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012106:Research; D014860:Warts / Q000235:genetics* / Q000628:therapy
D004279:DNA, Viral