Paeonol promoted 2-aminofluorene and p-aminobenzoic acid acetylations by mononuclear leucocytes from Sprague-Dawley rats.
Following exposure of rats to the arylamine carcinogen 2-aminofluorene, DNA-carcinogen adducts were found in the target tissues of the liver and bladder, and also in circulating leucocytes. This work investigated how paeonol affects arylamine (2-aminofluorene and p-aminobenzoic acid) acetylations in rat leucocytes. Evidence is presented showing that rat mononuclear leucocytes are capable of acetylating 2-aminofluorene and p-aminobenzoic acid. Paeonol promoted 2-aminofluorene and p-aminobenzoic acid acetylation. Cultured lymphocytes produced about twice as much N-acetyl-2-aminofluorene from 2-aminofluorene and 2.2-fold as much N-acetyl-p-aminobenzoic acid from p-aminobenzoic acid as monocytes. After cotreatment with paeonol, the lymphocyte and monocyte cultures indicated that paeonol did increase 2-aminofluorene and p-aminobenzoic acid acetylations.
Chang
H L
HL
Department of Pharmacology, China Medical College, Taichung, Taiwan, Republic of China.
Hung
C F
CF
Yeh
C C
CC
Chang
W C
WC
Chung
J G
JG
D010129:4-Aminobenzoic Acid / Q000378:metabolism* / Q000494:pharmacology; D000098:Acetophenones / Q000494:pharmacology*; D000107:Acetylation / Q000187:drug effects; D000818:Animals; D001191:Arylamine N-Acetyltransferase / Q000187:drug effects* / Q000378:metabolism; D002273:Carcinogens / Q000378:metabolism* / Q000494:pharmacology; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004610:Ellagic Acid / Q000494:pharmacology; D005449:Fluorenes / Q000378:metabolism* / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D000098:Acetophenones; D002273:Carcinogens; D005449:Fluorenes; D004610:Ellagic Acid; C012177:2-aminofluorene; C013638:paeonol; D001191:Arylamine N-Acetyltransferase; D010129:4-Aminobenzoic Acid