Solution structure of the amino acid sequence coded by the rarely expressed exon 26A of human elastin: the N-terminal region.
We previously reported the structural and biological properties of the C-terminal sequence (REGDPSSSQHLPSTPSSPRV) coded by the rarely expressed exon 26A of human elastin. It assumes a stable type II beta-turn structure spanning the REGD sequence and possesses chemotactic and immunological properties. Here the structural characterization of the sequence coded by this exon was completed. Nuclear magnetic resonance and circular dichroism studies on the N-terminal amino acid sequence (GADEGVRRSLSPELREGD) showed the presence of an alpha-helix within VRRSL and a type II beta-turn within SPEL. The smaller peptides GADEGVRRSLSP and LSPELREGD revealed structural features similar to those identified in the parent peptide. No beta-turn was found in the REGD sequence of these peptides and no chemotactic activity was detected, thereby demonstrating that this biological activity is conformation dependent. Structural studies on additional peptides such as LREGD, ELREGD and LSPELREGDPSS showed that the presence of a Glu residue two positions before the Arg residue inhibits the beta-turn formation in the REGD sequence.
Bisaccia
F
F
Department of Chemistry, University of Basilicata, Potenza, Italy.
Castiglione-Morelli
M A
MA
Spisani
S
S
Serafini-Fracassini
A
A
Tamburro
A M
AM
D000595:Amino Acid Sequence; D002630:Chemotactic Factors / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D002942:Circular Dichroism; D004549:Elastin / Q000737:chemistry* / Q000235:genetics*; D005091:Exons / Q000235:genetics*; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009000:Monocytes / Q000166:cytology / Q000187:drug effects; D019906:Nuclear Magnetic Resonance, Biomolecular; D010446:Peptide Fragments / Q000737:chemistry* / Q000235:genetics / Q000494:pharmacology; D017433:Protein Structure, Secondary
D002630:Chemotactic Factors; D010446:Peptide Fragments; D004549:Elastin