Primate foamy virus Pol proteins are imported into the nucleus.
Mouse monoclonal antibodies (MAbs) that specifically detect the 127 kDa Pol precursor and the 85 kDa reverse transcriptase/RNase H (RT/RN) or pr127 and the 40 kDa integrase (IN) in immunoblot and immunofluorescence assays (IFA) were used to investigate the subcellular localization of primate foamy virus (PFV) proteins. IFA of cells infected with PFV using the anti-Pol MAbs and rabbit anti-capsid (Gag) serum revealed that both the Gag and Pol proteins are transported into the nucleus. Transfection of cells with eukaryotic expression constructs for pr127(Pol), p85(RT/RN) and p40(IN) served to show Gag-independent subcellular localization of Pol proteins. Interestingly, not only the Pol precursor and IN molecules were found to be localized to the nucleus, but also the RT/RN subdomain. It is therefore suggested that PFV cores bear at least three separate nuclear localization signals, one in Gag and two in Pol. The latter appear to be localized to the two Pol subdomains.
Imrich
Horst
H
Institut für Virologie und Immunbiologie, Universität Würzburg, Germany1.
Heinkelein
Martin
M
Institut für Virologie und Immunbiologie, Universität Würzburg, Germany1.
Herchenröder
Ottmar
O
Institut für Virologie im MTZ, Medizinische Fakultät 'Carl Gustav Carus', Technische Universität Dresden, Fiedlerstr. 42, 01307 Dresden, Germany2.
Rethwilm
Axel
A
Institut für Virologie im MTZ, Medizinische Fakultät 'Carl Gustav Carus', Technische Universität Dresden, Fiedlerstr. 42, 01307 Dresden, Germany2.|Institut für Virologie und Immunbiologie, Universität Würzburg, Germany1.
D016475:3T3 Cells; D021581:Active Transport, Cell Nucleus; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D015153:Blotting, Western; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism* / Q000821:virology; D006224:Cricetinae; D005455:Fluorescent Antibody Technique; D015683:Gene Products, gag / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D015684:Gene Products, pol / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D019426:Integrases / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D019913:Nuclear Localization Signals; D011323:Primates / Q000821:virology*; D012194:RNA-Directed DNA Polymerase / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D016914:Ribonuclease H / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D016092:Spumavirus / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D014162:Transfection
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D015683:Gene Products, gag; D015684:Gene Products, pol; D019913:Nuclear Localization Signals; D019426:Integrases; D012194:RNA-Directed DNA Polymerase; D016914:Ribonuclease H