title: Dopamine-induced translocation of protein kinase C isoforms visualized in renal epithelial cells.
abstract: Short-term regulation of sodium metabolism is dependent on the modulation of the activity of sodium transporters by first and second messengers. In understanding diseases associated with sodium retention, it is necessary to identify the coupling between these messengers. We have examined whether dopamine, an important first messenger in tubular cells, activates and translocates various protein kinase C (PKC) isoforms. We used a proximal tubular-like cell line, LLCPK-1 cells, in which dopamine was found to inhibit Na(+)-K(+)-ATPase in a PKC-dependent manner. Translocation of PKC isoforms was studied with both subcellular fractionation and confocal microscopy. Both techniques revealed a dopamine-induced translocation from cytosol to plasma membrane of PKC-alpha and -epsilon, but not of PKC-delta, -gamma, and -zeta. The process of subcellular fractionation resulted in partial translocation of PKC-epsilon. This artifact was eliminated in confocal studies. Confocal imaging permitted detection of translocation within 20 s. Translocation was abolished by a phospholipase C inhibitor and by an antagonist against the dopamine 1 subtype (D(1)) but not the 2 subtype of receptor (D(2)). In conclusion, this study visualizes in renal epithelial cells a very rapid activation of the PKC-alpha and -epsilon isoforms by the D(1) receptor subtype.
authors.affiliation: Department of Woman and Child Health, Karolinska Institute, Astrid Lindgren Children's Hospital, S-171 76 Stockholm, Sweden.
authors.forename: S
authors.initials: S
authors.lastname: Nowicki
authors.forename: M S
authors.initials: MS
authors.lastname: Kruse
authors.forename: H
authors.initials: H
authors.lastname: Brismar
authors.forename: A
authors.initials: A
authors.lastname: Aperia
mesh_terms: D000818:Animals; D001552:Benzazepines / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects / Q000502:physiology; D004298:Dopamine / Q000494:pharmacology*; D018492:Dopamine Antagonists / Q000494:pharmacology; D004847:Epithelial Cells / Q000737:chemistry / Q000166:cytology / Q000201:enzymology; D004963:Estrenes / Q000494:pharmacology; D007527:Isoenzymes / Q000378:metabolism*; D007687:Kidney Tubules, Proximal / Q000737:chemistry / Q000166:cytology / Q000201:enzymology*; D018374:LLC-PK1 Cells; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism*; D051571:Protein Kinase C-alpha; D051745:Protein Kinase C-delta; D051744:Protein Kinase C-epsilon; D011760:Pyrrolidinones / Q000494:pharmacology; D017447:Receptors, Dopamine D1 / Q000378:metabolism; D017448:Receptors, Dopamine D2 / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013552:Swine; D010738:Type C Phospholipases / Q000378:metabolism; D000099221:Protein Kinase C zeta
chemical_list: D001552:Benzazepines; D004298:Dopamine; D018492:Dopamine Antagonists; D004963:Estrenes; D007527:Isoenzymes; D010726:Phosphodiesterase Inhibitors; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha; D051745:Protein Kinase C-delta; D051744:Protein Kinase C-epsilon; D011760:Pyrrolidinones; D017447:Receptors, Dopamine D1; D017448:Receptors, Dopamine D2; D000254:Sodium-Potassium-Exchanging ATPase; D010738:Type C Phospholipases; D000099221:Protein Kinase C zeta; C060229:1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione; C074854:U 73343; C087857:protein kinase C gamma