Gas chromatographic--mass spectrometric determination of etomidate in mouse brain.
A simple, rapid and reliable method was developed to determine the concentration of etomidate [ethyl-1-(1-phenylethyl)-1 H-imidazole-5-carboxylate] in mouse brain tissue by gas chromatography-mass spectrometry (GC-MS). Ethyl 5-amino-1-phenyl-4-pyrazolecarboxylate was used as internal standard (IS) and liquid-liquid extraction using ethyl ether as the solvent. The method demonstrated excellent recovery (93%) and a linear calibration range of 50-2500 ng/0.2 g. Intra-day accuracy and precision had an error and coefficient of variation of less than 8.7% and 4.2%, respectively. The limit of detection was 1 ng in mouse brain. Our results suggest that this new method is suitable for the quantitative analysis of etomidate in mouse brain tissue.
Deng
X
X
Department of Anesthesiology, University of Colorado Health Sciences Center, Denver, CO 80262, USA.
Simpson
V J
VJ
D018686:Anesthetics, Intravenous / Q000032:analysis*; D000818:Animals; D001923:Brain Chemistry; D005045:Etomidate / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008297:Male; D051379:Mice; D012680:Sensitivity and Specificity
D018686:Anesthetics, Intravenous; D005045:Etomidate