title: LldI, a plasmid-encoded type I restriction and modification system in Lactococcus lactis.
abstract: A plasmid-encoded type I restriction and modification (R-M) system, designated LldI, was identified in Lactococcus lactis biovar diacetylactis LD10-1. LldI consists of three genes encoding endonuclease, methylase and specificity subunits, respectively. RT-PCR analysis revealed that the three genes are co-transcribed as a polycistronic mRNA in L. lactis. The specificity subunit of LldI differs significantly in the target recognition domains from those of other type I R-M systems, suggesting that LldI confers a novel specificity in L. lactis.
authors.affiliation: Cooperative Research Centre for Food Industry Innovation, Department of Biotechnology, University of New South Wales, Sydney, Australia.
authors.forename: Y M
authors.initials: YM
authors.lastname: Deng
authors.forename: C Q
authors.initials: CQ
authors.lastname: Liu
authors.forename: N W
authors.initials: NW
authors.lastname: Dunn
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D017124:Conserved Sequence; D004262:DNA Restriction Enzymes / Q000737:chemistry / Q000235:genetics*; D015253:Deoxyribonucleases, Type I Site-Specific / Q000737:chemistry / Q000235:genetics*; D005798:Genes, Bacterial; D013294:Lactococcus lactis / Q000201:enzymology* / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D021122:Protein Subunits; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000737:chemistry / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D021122:Protein Subunits; D012333:RNA, Messenger; C421263:DNA modification methylase LldI; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); D004262:DNA Restriction Enzymes; C421262:deoxyendoribonuclease LldI; D015253:Deoxyribonucleases, Type I Site-Specific