Protein G expressed by human group C and G streptococci: cloning of gene and binding properties.
PCR generated fragments of the protein G gene from three GCS and GGS strains belonging to different G types had been cloned. The resulting PCR products were cloned into E. coli using expression vector pQE31. The clones, producing IgG-binding peptides were selected. Recombinant plasmids carried different inserts and encoded proteins of different size and with different binding properties.
Voltchek
N
N
Institute of Experimental Medicine, St. Petersburg, Russia.
Gupalova
T
T
Totolyan
A
A
D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D015870:Gene Expression; D005798:Genes, Bacterial; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D066298:In Vitro Techniques; D008970:Molecular Weight; D011485:Protein Binding; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013291:Streptococcus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
D001426:Bacterial Proteins; C034284:IgG Fc-binding protein, Streptococcus; D007074:Immunoglobulin G; D011994:Recombinant Proteins