Effect of the extract of Ginkgo biloba (EGb 761) on the circulating and cellular profiles of polyunsaturated fatty acids: correlation with the anti-oxidant properties of the extract.
Ginkgo biloba extract (EGb 761) has beneficial effects on cognitive functions in aging patients, and on various pathologies, including cardiovascular diseases. Although the extract is known to have antioxidant properties and improve membrane fluidity, the cellular mechanisms underlying these effects have not been determined. Here, we examined the in vivo effects of EGb 761 on circulating and cellular lipids. EGb 761 treatment induced significant increases in the levels of circulating polyunsaturated fatty acids (PUFAs), and a decrease in the saturation index SI (saturated/polyunsaturated species). Plasma triglycerides and cholesterol were not affected, while phospholipids were slightly increased at the higher dose of EGb 761. EGb 761 treatment also induced a significant increase in the levels of PUFAs in erythrocyte membranes, especially for the eicosapentaenoic acid (EPA omega 3), and a decrease in the saturation index. Moreover, the response of erythrocytes to oxidative stress was improved in EGb 761-treated animals (H(2)O(2)-induced cell lysis decreased by 50%). Considering that PUFAs are known to improve membrane fluidity and response to oxidative damage, and are precursors of signaling molecules such as prostaglandins, the effects of EGb 761 on circulating and cellular PUFAs may explain some of the pharmacological properties of Ginkgo biloba.
Drieu
K
K
Institut Henri Beaufour-Ipsen, 24 rue Erlanger, 75781, Paris Cedex 16, France. katy.drieu@beaufour-ipsen.com
Vranckx
R
R
Benassayad
C
C
Haourigi
M
M
Hassid
J
J
Yoa
R G
RG
Rapin
J R
JR
Nunez
E A
EA
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D002849:Chromatography, Gas; D015118:Eicosapentaenoic Acid / Q000097:blood; D004910:Erythrocyte Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D005227:Fatty Acids / Q000097:blood; D005231:Fatty Acids, Unsaturated / Q000097:blood*; D020441:Ginkgo biloba / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D010743:Phospholipids / Q000097:blood; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000097:blood
D000975:Antioxidants; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D010743:Phospholipids; D010936:Plant Extracts; D014280:Triglycerides; D002784:Cholesterol; D015118:Eicosapentaenoic Acid; D006861:Hydrogen Peroxide