title: Assaying DNA topoisomerase I relaxation activity.
authors.affiliation: Department of Microbiology, University of Washington, Seattle, USA.
authors.forename: L
authors.initials: L
authors.lastname: Stewart
authors.forename: J J
authors.initials: JJ
authors.lastname: Champoux
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism / Q000494:pharmacology; D002021:Buffers; D002457:Cell Extracts; D004264:DNA Topoisomerases, Type I / Q000032:analysis* / Q000145:classification / Q000378:metabolism*; D004278:DNA, Superhelical / Q000737:chemistry / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D009994:Osmolar Concentration; D016012:Poisson Distribution; D011994:Recombinant Proteins; D012492:Salts / Q000494:pharmacology
chemical_list: D002021:Buffers; D002457:Cell Extracts; D004278:DNA, Superhelical; D011994:Recombinant Proteins; D012492:Salts; D000255:Adenosine Triphosphate; D004264:DNA Topoisomerases, Type I; D008274:Magnesium