The establishment of a human liver nuclei two-dimensional electrophoresis reference map.
This short communication describes the establishment of a two-dimensional electrophoresis (2-DE) reference map of nuclear proteins isolated from human liver. The human liver nuclei 2-DE reference map contains 1497 spots. In an initial identification study using peptide mass fingerprinting as a means of protein identification we were able to identify 26 spots corresponding to 15 different proteins. The human liver nuclei 2-DE reference map is now included in the SWISS-2DPAGE database, which can be accessed through the ExPASy server (http://www.expasy.ch/ch2d/).
Jung
E
E
Central Clinical Chemistry Laboratory, Geneva University Hospital, Switzerland. eva.jung@dim.hcuge.ch
Hoogland
C
C
Chiappe
D
D
Sanchez
J C
JC
Hochstrasser
D F
DF
D002467:Cell Nucleus / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008099:Liver / Q000378:metabolism* / Q000648:ultrastructure; D009687:Nuclear Proteins / Q000302:isolation & purification / Q000378:metabolism
D009687:Nuclear Proteins