title: Surface-enhanced Raman scattering: a structure-specific detection method for capillary electrophoresis.
abstract: A new approach to detecting capillary electrophoresis (CE) eluent components by interfacing CE with a surface-enhanced Raman scattering (SERS) system is described. In this approach, CE-based separation of a mixture of trans-1,2-bis(4-pyridyl)ethylene and N,N-dimethyl-4-nitrosoaniline has been detected by SERS in a postcolumn geometry. The retention time obtained from SERS corresponds well with that from conventional UV-visible detection. Meanwhile, CE eluants are identified by their characteristic vibrational spectra, demonstrating the validity of SERS as a structure-specific detection method for CE. In addition, the ability to monitor SERS intensity changes at molecule-specific frequencies makes selective detection of individual analytes possible, even when separation is incomplete. Finally, CE-SERS is evaluated for separation of amino acids (tyrosine and tryptophan) and environmental pollutants (chlorophenol mixtures).
authors.affiliation: Department of Chemistry, Pennsylvania State University, University Park 16802-6300, USA.
authors.forename: L
authors.initials: L
authors.lastname: He
authors.forename: M J
authors.initials: MJ
authors.lastname: Natan
authors.forename: C D
authors.initials: CD
authors.lastname: Keating
mesh_terms: D000814:Aniline Compounds / Q000032:analysis; D002733:Chlorophenols; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D009603:Nitroso Compounds / Q000032:analysis; D011725:Pyridines / Q000032:analysis; D012834:Silver; D013053:Spectrophotometry; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*; D014364:Tryptophan / Q000032:analysis; D014443:Tyrosine / Q000032:analysis
chemical_list: D000814:Aniline Compounds; D002733:Chlorophenols; D009603:Nitroso Compounds; D011725:Pyridines; C008651:4-nitrosodimethylaniline; D012834:Silver; D014443:Tyrosine; D014364:Tryptophan; C023650:aniline