Quantitative trait loci mapping for cholesterol gallstones in AKR/J and C57L/J strains of mice.
Quantitative trait locus (QTL) mapping was used to locate genes that determine the difference in cholesterol gallstone disease between the gallstone-susceptible strain C57L/J and the gallstone-resistant strain AKR/J. Gallstone weight was determined in 231 male (AKR x C57L) F(1) x AKR backcross mice fed a lithogenic diet containing 1% cholesterol, 0.5% cholic acid, and 15% butterfat for 8 wk. Mice having no stones and mice having the largest stones were genotyped at approximately 20-cM intervals to find the loci determining cholesterol gallstone formation. The major locus, Lith1, mapped near D2Mit56 and was confirmed by constructing a congenic strain, AK. L-Lith1(s). Another locus, Lith2, mapped near D19Mit58 and was also confirmed by constructing a congenic strain AK.L-Lith2(s). Other suggestive, but not statistically significant, loci mapped to chromosomes 6, 7, 8, 10, and X. The identification of these Lith genes will elucidate the pathophysiology of cholesterol gallstone formation.
Paigen
B
B
The Jackson Laboratory, Bar Harbor, Maine 04609, USA. bjp@jax.org
Schork
N J
NJ
Svenson
K L
KL
Cheah
Y C
YC
Mu
J L
JL
Lammert
F
F
Wang
D Q
DQ
Bouchard
G
G
Carey
M C
MC
D000818:Animals; D002769:Cholelithiasis / Q000235:genetics*; D002784:Cholesterol / Q000235:genetics; D002791:Cholesterol, Dietary / Q000009:adverse effects; D002874:Chromosome Mapping / Q000379:methods; D003433:Crosses, Genetic; D005704:Gallbladder / Q000737:chemistry / Q000503:physiopathology; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease / Q000235:genetics; D008297:Male; D051379:Mice; D008806:Mice, Inbred AKR; D008810:Mice, Inbred C57BL; D009929:Organ Size / Q000235:genetics; D019655:Quantitative Trait, Heritable
D002791:Cholesterol, Dietary; D005819:Genetic Markers; D002784:Cholesterol