title: Automated enzymatic mitochondrial antibody assay for the diagnosis of primary biliary cirrhosis.
abstract: Primary biliary cirrhosis is a progressive autoimmune disease that affects middle aged women, resulting in liver cirrhosis. We describe here an automated enzymatic mitochondrial antibody assay adapted for performance on laboratory analysers for the serological diagnosis of primary biliary cirrhosis. This assay detects the characteristic autoantibody directed against the 74kDa E2 subunit of the pyruvate dehydrogenase complex. Analysis of receiver operator characteristic curve data indicated that the automated enzymatic mitochondrial assay procedure discriminated clinically identified patients with primary biliary cirrhosis from normal subjects with a sensitivity of 83% and a specificity of 100%. This method compared favourably against a commercial ELISA method which had a sensitivity of 73% and a specificity of 100%. The automated enzymatic mitochondrial antibody assay is a high throughput assay of use for the routine diagnosis of patients with primary biliary cirrhosis with autoantibodies to the E2 subunit of the pyruvate dehydrogenase complex. The method is of potential value for economical and rapid screening to detect asymptomatic primary biliary cirrhosis in the at-risk segment of the population, namely middle aged women.
authors.affiliation: TRACE Scientific Ltd., Noble Park, Victoria, Australia. wayne_jensen@tracesci.com
authors.forename: W A
authors.initials: WA
authors.lastname: Jensen
authors.forename: J A
authors.initials: JA
authors.lastname: Jois
authors.forename: P
authors.initials: P
authors.lastname: Murphy
authors.forename: J
authors.initials: J
authors.lastname: De Giorgio
authors.forename: B
authors.initials: B
authors.lastname: Brown
authors.forename: M J
authors.initials: MJ
authors.lastname: Rowley
authors.forename: I R
authors.initials: IR
authors.lastname: Mackay
mesh_terms: D001323:Autoantibodies / Q000097:blood / Q000276:immunology; D001324:Autoantigens / Q000737:chemistry / Q000276:immunology; D001327:Autoimmune Diseases / Q000175:diagnosis* / Q000201:enzymology / Q000276:immunology; D001331:Automation; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation / Q000379:methods*; D008105:Liver Cirrhosis, Biliary / Q000175:diagnosis* / Q000201:enzymology / Q000276:immunology; D008875:Middle Aged; D008930:Mitochondria, Liver / Q000201:enzymology* / Q000276:immunology; D008970:Molecular Weight; D021122:Protein Subunits; D011768:Pyruvate Dehydrogenase Complex / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D012372:ROC Curve; D012680:Sensitivity and Specificity
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D021122:Protein Subunits; D011768:Pyruvate Dehydrogenase Complex