title: The human Hsp70B gene at the HSPA7 locus of chromosome 1 is transcribed but non-functional.
abstract: The human heat-inducible Hsp70B and Hsp70B' genes were co-localized to 1q23.1 by in situ hybridization. However, though transcripts from Hsp70B could be detected in heat-shocked cells, DNA sequence analyses of both the gene and cDNA copies of the mRNA indicate the gene is non-functional. Moreover, mouse homologues of Hsp70B/B' were not detected by Southern blot analysis, suggesting Hsp70B/B' arose from either Hsp70-1or Hsp70-2 after the divergence of mice and humans.
authors.affiliation: Department of Radiation Oncology, Washington University School of Medicine, St. Louis, MO 63108, USA.
authors.forename: A J
authors.initials: AJ
authors.lastname: Parsian
authors.forename: J E
authors.initials: JE
authors.lastname: Sheren
authors.forename: T Y
authors.initials: TY
authors.lastname: Tao
authors.forename: P C
authors.initials: PC
authors.lastname: Goswami
authors.forename: R
authors.initials: R
authors.lastname: Malyapa
authors.forename: R
authors.initials: R
authors.lastname: Van Rheeden
authors.forename: M S
authors.initials: MS
authors.lastname: Watson
authors.forename: C R
authors.initials: CR
authors.lastname: Hunt
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics*; D019143:Evolution, Molecular; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics* / Q000502:physiology*; D018869:Heat-Shock Response / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: D018840:HSP70 Heat-Shock Proteins; C491129:HSPA7 protein, human; D012333:RNA, Messenger