Genetic analysis of peptidoglycan biosynthesis in mycobacteria: characterization of a ddlA mutant of Mycobacterium smegmatis.
A temperature-sensitive mutant of Mycobacterium smegmatis was characterized that contains a mutation in ddlA, the gene encoding D-alanine:D-alanine ligase. Enzymatic assays using recombinant proteins and D-cycloserine susceptibility indicate that the A365V mutation in the SMEG35 DdlA protein causes a reduction in enzymatic activity in vitro and in vivo.
Belanger
A E
AE
Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA.
Porter
J C
JC
Hatfull
G F
GF
D001483:Base Sequence; D004269:DNA, Bacterial; D015870:Gene Expression; D008969:Molecular Sequence Data; D016296:Mutagenesis; D020102:Mycobacterium smegmatis / Q000201:enzymology* / Q000235:genetics; D010453:Peptide Synthases / Q000235:genetics* / Q000378:metabolism; D010457:Peptidoglycan / Q000096:biosynthesis*; D013696:Temperature
D004269:DNA, Bacterial; D010457:Peptidoglycan; D010453:Peptide Synthases; C019638:D-alanylalanine synthetase