title: P2X1 receptor subunit immunoreactivity and ATP-evoked fast currents in adult rat dorsal root ganglion neurons.
abstract: Triple fluorescent staining for P2X1 and P2X3 subunits and isolectin I-B4 (IB4) were performed on acutely dissociated rat DRG neurons. Immunoreactivity for P2X1 and P2X3 subunits was present separately or together in DRG neurons. P2X1 immunoreactivity was present in both IB4-positive and IB4-negative cells. When combining patch-clamp recordings with immunostaining for the P2X1 and P2X3 subunits on single recorded cells, ATP-evoked fast currents were shown to be present on DRG neurons that have immunoreactivity for the P2X3 subunit only, the P2X1 subunit only, or both P2X1 and P2X3 subunits. These results raised a possibility that, in addition to the P2X3 receptor subunit, the P2X1 subunit may also contribute to functional P2X receptors with fast kinetics in DRG neurons.
authors.affiliation: McKnight Brain Institute of the University of Florida Gainesville 32610, USA.
authors.forename: J C
authors.initials: JC
authors.lastname: Petruska
authors.forename: N
authors.initials: N
authors.lastname: Mena
authors.forename: T
authors.initials: T
authors.lastname: Nakatsuka
authors.forename: B Y
authors.initials: BY
authors.lastname: Cooper
authors.forename: R D
authors.initials: RD
authors.lastname: Johnson
authors.forename: J G
authors.initials: JG
authors.lastname: Gu
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D005071:Evoked Potentials / Q000187:drug effects / Q000502:physiology*; D005727:Ganglia, Spinal / Q000166:cytology / Q000502:physiology*; D007150:Immunohistochemistry; D007700:Kinetics; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D018408:Patch-Clamp Techniques; D051381:Rats; D018048:Receptors, Purinergic P2 / Q000032:analysis / Q000502:physiology*; D058469:Receptors, Purinergic P2X; D058477:Receptors, Purinergic P2X3
chemical_list: C546410:P2rx3 protein, rat; D018048:Receptors, Purinergic P2; D058469:Receptors, Purinergic P2X; D058477:Receptors, Purinergic P2X3; D000255:Adenosine Triphosphate