title: De novo design of helical bundles as models for understanding protein folding and function.
abstract: De novo protein design has proven to be a powerful tool for understanding protein folding, structure, and function. In this Account, we highlight aspects of our research on the design of dimeric, four-helix bundles. Dimeric, four-helix bundles are found throughout nature, and the history of their design in our laboratory illustrates our hierarchic approach to protein design. This approach has been successfully applied to create a completely native-like protein. Structural and mutational analysis allowed us to explore the determinants of native protein structure. These determinants were then applied to the design of a dinuclear metal-binding protein that can now serve as a model for this important class of proteins.
authors.affiliation: Johnson Research Foundation, Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6059, USA.
authors.forename: R B
authors.initials: RB
authors.lastname: Hill
authors.forename: D P
authors.initials: DP
authors.lastname: Raleigh
authors.forename: A
authors.initials: A
authors.lastname: Lombardi
authors.forename: W F
authors.initials: WF
authors.lastname: DeGrado
mesh_terms: D055598:Chemical Phenomena; D002627:Chemistry, Physical; D017510:Protein Folding; D017433:Protein Structure, Secondary; D011506:Proteins / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D011506:Proteins