DNA accessibility to minor groove ligands in core nucleosome and chromatosome.
Using the circular dichroism spectra induced in the visible by the binding to the minor groove of DNA, we found that Hoechst 33258 is able to occupy its specific sites even when these are located inside the nucleosome structure. This high accessibility of the DNA in the nucleosome is not modified by the removal of the amino-terminal domains of the octamer histones and is not reduced by the presence of linker histone. Interesting and reasonable differences were found in the association constants.
Foderà
R
R
CNR Centro di Studio per gli Acidi Nucleici, Università di Roma La Sapienza, Rome, Italy.
Caneva
R
R
Canzonetta
C
C
Savino
M
M
D000818:Animals; D001665:Binding Sites; D006690:Bisbenzimidazole / Q000378:metabolism*; D002645:Chickens; D002843:Chromatin / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D002942:Circular Dichroism; D004247:DNA / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D005456:Fluorescent Dyes / Q000378:metabolism; D006657:Histones / Q000737:chemistry; D007211:Indoles / Q000378:metabolism*; D008024:Ligands; D009707:Nucleosomes / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D017434:Protein Structure, Tertiary
D002843:Chromatin; D005456:Fluorescent Dyes; D006657:Histones; D007211:Indoles; D008024:Ligands; D009707:Nucleosomes; C007293:DAPI; D004247:DNA; D006690:Bisbenzimidazole