Determination of deramciclane and N-desmethylderamciclane in human plasma by liquid chromatography-tandem mass spectrometry using off-line robotic sample pretreatment.
A rapid and highly sensitive LC-MS-MS method using deuterium-labelled internal standards was developed and evaluated for the simultaneous determination of deramciclane and its pharmacologically active metabolite (N-desmethylderamciclane). The sample preparation based on liquid-liquid extraction was carried out with an off-line robotic system. Evaluation of this analytical method shows that samples can be assayed with acceptable accuracy and precision in the 0.1 to 50 ng/ml concentration range for both compounds. The method was applied for the quantitative determination of deramciclane and its metabolite in human plasma samples during a food interaction pharmacokinetic study.
Tolokán
A
A
Budapest University of Technology and Economics, Division of Chemical Information Technology, Hungary. toni.kit@chem.bme.hu
Horváth
V
V
Horvai
G
G
Egresi
A
A
Nemes
K B
KB
Klebovich
I
I
D002138:Calibration; D001892:Camphanes / Q000097:blood* / Q000493:pharmacokinetics; D002853:Chromatography, Liquid / Q000379:methods*; D018565:Food-Drug Interactions; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results; D012371:Robotics; D018490:Serotonin Agents / Q000097:blood*
D001892:Camphanes; C406721:N-desmethylderamciclane; D018490:Serotonin Agents; C060555:deramciclane