Site-directed mutagenesis of large (13-kb) plasmids in a single-PCR procedure.
Wang
J
J
University of Texas M.D. Anderson Cancer Center, Houston, USA.
Wilkinson
M F
MF
D000818:Animals; D003001:Cloning, Molecular / Q000379:methods; D004252:DNA Mutational Analysis; D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D051379:Mice; D016297:Mutagenesis, Site-Directed; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*
D017931:DNA Primers; D011933:Reagent Kits, Diagnostic; D011948:Receptors, Antigen, T-Cell; D004259:DNA-Directed DNA Polymerase