title: Electron-microscopic detection of apoptotic and necrotic cell death in non-atherosclerotic areas of stenotic aortocoronary saphenous vein bypass grafts.
abstract: Aortocoronary saphenous vein bypass grafts undergo structural alterations within the arterialized vein, resulting in graft stenosis and failure. Areas of the acellular intima contribute to fissuring, cracking and ulceration, while areas of the media become highly vascular but thinned. This study aimed to examine the ultrastructural features of cell death, including apoptosis and necrosis, in non-atherosclerotic areas of the stenotic aortocoronary saphenous vein bypass grafts. Thirteen stenotic vein grafts were obtained at redo coronary artery bypass grafting. The ultrastructural features of cell death were analysed by electron microscopy. Typical features of necrosis, including focal areas of cytoplasmic oedema, plasmalemmal destruction and nuclear condensation with cytoplasmic organelle destruction, were observed throughout the intima and media. Features of apoptosis, including the presence of apoptotic bodies, were also identified in the hyperplastic intima and its adjacent media. Our observations suggest that both apoptosis and necrosis occur in non-atherosclerotic areas of stenotic aortocoronary saphenous vein bypass grafts.
authors.affiliation: Surgical Professorial Unit, St. Vincent's Hospital, Darlinghurst, NSW, Australia.
authors.forename: A Y
authors.initials: AY
authors.lastname: Wang
authors.forename: Y V
authors.initials: YV
authors.lastname: Bobryshev
authors.forename: H
authors.initials: H
authors.lastname: Liang
authors.forename: S M
authors.initials: SM
authors.lastname: Cherian
authors.forename: S J
authors.initials: SJ
authors.lastname: Inder
authors.forename: K W
authors.initials: KW
authors.lastname: Ashwell
authors.forename: A E
authors.initials: AE
authors.lastname: Farnsworth
authors.forename: R S
authors.initials: RS
authors.lastname: Lord
mesh_terms: D000368:Aged; D017209:Apoptosis; D001161:Arteriosclerosis / Q000473:pathology; D016923:Cell Death; D001026:Coronary Artery Bypass; D003327:Coronary Disease / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D009336:Necrosis; D012501:Saphenous Vein / Q000473:pathology* / Q000648:ultrastructure