title: Expression of an intracisternal A-particle-like element in rat ovary.
abstract: We have isolated a rat intracisternal-A particle element (IAP)-like element (IAP-LE) from ovarian granulosa cells that appears to be identical to the rat EST clone AA964260. The compiled cDNA sequences contain several putative in-frame translation initiation codons with the largest capable of encoding a 365 amino acid protein with a reverse transcriptase domain in the N-terminus as well as a bipartite nuclear localization signal sequence in the middle. Northern blotting shows a major approximately 7 Kb transcript and a minor approximately 5 Kb transcript that are abundantly expressed in the ovary. In situ hybridization histochemistry using ovaries from gonadotropin-treated immature rats and regularly cycling adult rats show that this transcript is predominantly localized to granulosa cells of all healthy follicles, including primary follicles, and to newly-formed and healthy corpora lutea. This cell-specific expression pattern of the IAP-LE gene is distinct from those of the several known retroviral elements, suggesting the potentially novel functional importance of the IAP-LE gene. Taken together, our results demonstrate abundant and cell-specific expression of a novel IAP-LE in rat granulosa cells.
authors.affiliation: Department of Physiology, University of Kentucky, Lexington, Kentucky 40536-0084, USA.
authors.forename: K M
authors.initials: KM
authors.lastname: Graham
authors.forename: C
authors.initials: C
authors.lastname: Ko
authors.forename: K S
authors.initials: KS
authors.lastname: Park
authors.forename: K
authors.initials: K
authors.lastname: Sarge
authors.forename: O K
authors.initials: OK
authors.lastname: Park-Sarge
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002453:Cell Cycle; D002467:Cell Nucleus / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D020224:Expressed Sequence Tags; D005260:Female; D007426:Genes, Intracisternal A-Particle / Q000235:genetics*; D006062:Gonadotropins / Q000494:pharmacology; D006064:Gonadotropins, Equine / Q000494:pharmacology; D006107:Granulosa Cells / Q000378:metabolism*; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D010053:Ovary / Q000378:metabolism*; D014176:Protein Biosynthesis; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D012689:Sequence Homology, Nucleic Acid; D013997:Time Factors; D014018:Tissue Distribution
chemical_list: D018076:DNA, Complementary; D006062:Gonadotropins; D006064:Gonadotropins, Equine; D012333:RNA, Messenger; D012194:RNA-Directed DNA Polymerase