Combined liquid and gas chromatographic characterisation of polyglycerol fatty acid esters.
In the present study a combined liquid and gas chromatographic technique is described for the analysis of polyglycerol fatty acid esters. Liquid chromatographic fractionation of samples resulted in pure standards of monoesters of di- and triglycerols and diesters of di- and triglycerols. Confirmation of their identity was achieved by LC-MS analysis. Moreover, a chromatographic identification of the mono- and diesters of cyclic diglycerol was proposed. From the isolation of pure esters and their gas chromatographic analysis, it was revealed that co-elution of several compounds occurred. Thus it was shown that prefractionation of the sample using a simplified liquid chromatographic separation, was necessary in order to characterise the esters correctly. In combination with some other chemical analyses, a complete profile of the chemical composition of polyglycerol fatty acid esters can be obtained.
De Meulenaer
B
B
Department of Food Technology and Nutrition, Ghent University, Belgium.
Van Royen
G
G
Vanhoutte
B
B
Huyghebaert
A
A
D002849:Chromatography, Gas / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D004952:Esters / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry*; D005990:Glycerol / Q000737:chemistry; D013058:Mass Spectrometry
D004952:Esters; D005227:Fatty Acids; D005990:Glycerol