title: Quantitative imaging of lateral ErbB1 receptor signal propagation in the plasma membrane.
abstract: Evidence for a new signaling mechanism consisting of ligand-independent lateral propagation of receptor activation in the plasma membrane is presented. We visualized the phosphorylation of green fluorescent protein (GFP)-tagged ErbB1 (ErbB1-GFP) receptors in cells focally stimulated with epidermal growth factor (EGF) covalently attached to beads. This was achieved by quantitative imaging of protein reaction states in cells by fluorescence resonance energy transfer (FRET) with global analysis of fluorescence lifetime imaging microscopy (FLIM) data. The rapid and extensive propagation of receptor phosphorylation over the entire cell after focal stimulation demonstrates a signaling wave at the plasma membrane resulting in full activation of all receptors.
authors.affiliation: Cell Biology and Cell Biophysics Program, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
authors.forename: P J
authors.initials: PJ
authors.lastname: Verveer
authors.forename: F S
authors.initials: FS
authors.lastname: Wouters
authors.forename: A R
authors.initials: AR
authors.lastname: Reynolds
authors.forename: P I
authors.initials: PI
authors.lastname: Bastiaens
mesh_terms: D001152:Arsenicals / Q000494:pharmacology; D002232:Carbocyanines; D002462:Cell Membrane / Q000378:metabolism*; D004058:Diffusion; D019281:Dimerization; D004705:Endocytosis; D004735:Energy Transfer; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000378:metabolism* / Q000494:pharmacology; D066246:ErbB Receptors / Q000378:metabolism*; D005453:Fluorescence; D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins; D006801:Humans; D007140:Immunoglobulin Fab Fragments; D008024:Ligands; D008164:Luminescent Proteins; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008863:Microspheres; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000276:immunology; D017027:Protein Tyrosine Phosphatases / Q000037:antagonists & inhibitors / Q000378:metabolism; D015398:Signal Transduction; D014407:Tumor Cells, Cultured
chemical_list: D001152:Arsenicals; D002232:Carbocyanines; D004791:Enzyme Inhibitors; D005456:Fluorescent Dyes; D007140:Immunoglobulin Fab Fragments; D008024:Ligands; D008164:Luminescent Proteins; C089187:cyanine dye 3; C029341:oxophenylarsine; D049452:Green Fluorescent Proteins; D019000:Phosphotyrosine; D004815:Epidermal Growth Factor; D066246:ErbB Receptors; D017027:Protein Tyrosine Phosphatases