Identification and characterization of a cDNA clone encoding the heat shock protein (Hsp60) from the biting midge, Culicoides variipennis sonorensis Wirth and Jones.
A cDNA expression library constructed from Culicoides variipennis sonorensis was screened using an antibody specific for Hsp60 of Heliothis virescens. A single clone encoding the complete heat shock protein (Hsp60) of C. variipennis was identified and its 2400-bp insert was sequenced. The encoded 62-kDa protein contains 581 amino acids and includes a 26-amino acid putative mitochondrial targeting sequence at its N terminus and a GGM motif at its carboxyl terminus. Deduced amino acid sequences are highly similar (67-78%) to Hsp60 of other species, including the fruit fly, the house mouse, the Norwegian rat, the Chinese hamster, the human, a nematode, and the tobacco budworm moth. This is the initial isolation of a coding sequence for a stress-induced protein in C. variipennis.
Abdallah
M A
MA
Department of Microbiology and Molecular Cell Sciences, University of Memphis, Tennessee 38152, USA.
Pollenz
R S
RS
Nunamaker
R A
RA
Murphy
K E
KE
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002519:Ceratopogonidae / Q000235:genetics*; D018834:Chaperonin 60 / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D003080:Cold Temperature; D006801:Humans; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D018834:Chaperonin 60; D019476:Insect Proteins