Sequence of a gene from Bombina orientalis coding for the antimicrobial peptide BLP-7.
The structure of a gene coding for bombinin-like peptides (BLP) in Bombina orientalis was determined. It comprises two exons separated by a 1337 bp intron. Exon 1 codes for the signal peptide, while exon 2 contains the genetic information for BLP-7 and a bombinin H-type peptide (GH-2). The promoter region contains putative recognition sites for nuclear factors, such as NF-IL6 and NF-kappaB. The analysis of the structure of this gene, compared with that of the previously reported BLP-3 gene sequence, suggests the occurrence of a gene duplication event, rather than an alternative splicing mechanism, which leads to the generation of both inter- and intra-families variability in this class of cytolytic peptides. Furthermore, chromosome walking analysis indicates that this gene family is not densely clustered.
Miele
R
R
Dipartimento di Scienze Biochimiche 'A. Rossi Fanelli' and CNR Centro di Biologia Molecolare, Università 'La Sapienza', Piazzale Aldo Moro 5, 00185, Rome, Italy.
Borro
M
M
Fiocco
D
D
Barra
D
D
Simmaco
M
M
D000595:Amino Acid Sequence; D029845:Amphibian Proteins; D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry*; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000235:genetics*; D001001:Anura; D001483:Base Sequence; D001665:Binding Sites; D015139:Blotting, Southern; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000235:genetics; D016386:Chromosome Walking; D005091:Exons; D007438:Introns; D008957:Models, Genetic; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000235:genetics; D010455:Peptides / Q000737:chemistry / Q000235:genetics*; D011401:Promoter Regions, Genetic; D021382:Protein Sorting Signals; D017422:Sequence Analysis, DNA
D029845:Amphibian Proteins; D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; C420363:BLP-7 protein, Bombina orientalis; D022782:CCAAT-Enhancer-Binding Protein-beta; D016328:NF-kappa B; D010455:Peptides; D021382:Protein Sorting Signals; C071930:bombinin-like peptide-2, Bombina orientalis; C071931:bombinin-like peptide-3, Bombina orientalis