Alteration in the expression of protein kinase C isoforms in human hepatocellular carcinoma.
This study was designed to investigate the alterations of individual protein kinase C (PKC) isoforms in human liver cancer. Surgical specimens of hepatocellular carcinoma and adjacent normal tissues were extracted into cytosolic and membranous fractions. The level of membrane-bound PKCalpha in the cancer tissue was significantly lower than that in the adjacent normal tissue and consistent with the change in PKC activity. In addition, there was a significant negative correlation between PKCalpha and tumor size. In both cytosolic and membrane fractions, levels of PKCdelta and PKCzeta was significantly higher in the cancer tissue than those in the adjacent normal liver tissue. The alterations in the PKC isoforms signify their roles in the hyperproliferation in liver cancer.
Tsai
J H
JH
Department of Nursing, Fooyin Institute of Technology, ROC, Kaohsiung 831, Taiwan.
Hsieh
Y S
YS
Kuo
S J
SJ
Chen
S T
ST
Yu
S Y
SY
Huang
C Y
CY
Chang
A C
AC
Wang
Y W
YW
Tsai
M T
MT
Liu
J Y
JY
D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002455:Cell Division; D002462:Cell Membrane / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D007527:Isoenzymes / Q000096:biosynthesis / Q000737:chemistry; D008099:Liver / Q000378:metabolism; D008113:Liver Neoplasms / Q000378:metabolism*; D020033:Protein Isoforms; D011493:Protein Kinase C / Q000096:biosynthesis* / Q000737:chemistry*; D051571:Protein Kinase C-alpha; D051745:Protein Kinase C-delta; D000099221:Protein Kinase C zeta
D007527:Isoenzymes; D020033:Protein Isoforms; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha; D051745:Protein Kinase C-delta; D000099221:Protein Kinase C zeta; C496628:PRKCA protein, human; C497258:PRKCD protein, human