Epinephrine intracerebroventricular stimulation modifies the LH effect on ovarian progesterone and androstenedione release.
This study investigates the interaction between the effect of epinephrine intracerebroventricular (icv) injection and LH on the progesterone concentration in ovarian vein blood (Po) in vivo, and also, on the release of ovarian progesterone and androstenedione in vitro, in rats on dioestrus day 2. When 2 mg ovine LH were injected in vein (i.v.), Po increased reaching 120+/-12.2 and 151+/-17.5 ng ml(-1) at 22 and 25 min, respectively. Another group of rats was injected intracerebroventricular with 5 microgram epinephrine at time zero, and with 2 mg ovine LH i.v. 3 min later. This time Po decreased during the first 3 min, then increased, reaching 64+/-7.1 ng ml(-1) at 25 min, lower than the Po obtained 22 min after LH i.v. injection only (P<0.01). Moreover, rats were injected i.v. with 2 mg ovine LH at time zero, and 7 min later with epinephrine intracerebroventricular. Po increased during the first 7 min, decreased until the 13th minute and reached 70+/-8.9 ng ml(-1) at 25 min, lower than the Po obtained 25 min after LH i.v. injection only (P<0.01). In other experience, rats with one (either right or left) superior ovarian nerve transected (SON-t), were injected intracerebroventricular with epinephrine. Five minutes later, the ovaries were removed and incubated in vitro with LH. Both ovaries (right or left) in which the SON was intact at time of epinephrine i. c.v. injection, showed a reduction of progesterone and androstenedione released in vitro (P<0.05). These results suggest that, on dioestrus day 2, the central adrenergic stimulus competes with LH in the release of ovarian progesterone. Also, the neural input that arrives at the ovary through the SON would antagonize the ovarian progesterone and androstenedione response to LH.
De Bortoli
M A
MA
Laboratorio de Biología de la Reproducción (LABIR), Universidad Nacional de San Luis (UNSL), Chacabuco 917, 5700, San Luis, Argentina. midebort@unsl.edu.ar
Garraza
M H
MH
Aguado
L I
LI
D000735:Androstenedione / Q000378:metabolism*; D000818:Animals; D004837:Epinephrine / Q000008:administration & dosage* / Q000494:pharmacology*; D005260:Female; D007276:Injections, Intraventricular; D007986:Luteinizing Hormone / Q000494:pharmacology*; D010053:Ovary / Q000098:blood supply* / Q000187:drug effects* / Q000294:innervation / Q000378:metabolism; D011374:Progesterone / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012756:Sheep; D013997:Time Factors; D014680:Veins / Q000187:drug effects / Q000378:metabolism
D000735:Androstenedione; D011374:Progesterone; D007986:Luteinizing Hormone; D004837:Epinephrine