title: A novel uterine lipocalin supporting pregnancy in equids.
abstract: Horses, donkeys, and therefore, probably all equids, secrete a nonglycosylated, progesterone-dependent, 19-kDa protein (P19) into the uterine lumen during early pregnancy, and significant quantities of it are taken up by the developing conceptus. Sequence analysis and structural modelling have identified P19 as a lipocalin with greatest similarity to the murine major urinary protein lipocalins. However, lack of strong identity with any particular group of lipocalins and several unusual structural features, including a unique amino acid triplet within one of the invariant domains and an unusual external tryptophan residue, classify it as a new member of the lipocalin family. P19 is therefore likely to be a transport protein involved in supporting early embryonic development. Preliminary evidence using recombinant-derived P19 and fluorescently tagged ligands suggests that it may transport a fatty acid or retinol-like molecule. Although an initial search failed to identify homologues of P19 in other mammals, they may nevertheless exist but are synthesised and secreted in much smaller quantities, making them difficult to detect. Equids appear to need particularly large quantities of the protein during early pregnancy because of the unusually late implantation in this species and the presence of a capsule surrounding the conceptus until about day 23 of gestation.
authors.affiliation: Developmental Genetics, The Babraham Institute, Cambridge, United Kingdom. francesca.stewart@bbsrc.ac.uk
authors.forename: F
authors.initials: F
authors.lastname: Stewart
authors.forename: M W
authors.initials: MW
authors.lastname: Kennedy
authors.forename: S
authors.initials: S
authors.lastname: Suire
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000502:physiology*; D018397:Equidae / Q000502:physiology*; D005260:Female; D006736:Horses; D054834:Lipocalins; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000502:physiology*; D011270:Pregnancy, Animal / Q000502:physiology*; D014599:Uterus / Q000502:physiology*
chemical_list: D002352:Carrier Proteins; D054834:Lipocalins; D011257:Pregnancy Proteins; C102507:uterine lipocalin, horse