title: Lesion-induced regulation of netrin receptors and modification of netrin-1 expression in the retina of fish and grafted rats.
abstract: To determine whether netrin receptors (DCC, UNC5H1, UNC5H2) and netrin-1 are present in the adult rat retina and may affect regeneration of retinal ganglion cell (RGC) axons into peripheral nerve (PN) grafts, in situ hybridization (ISH) and immunostaining experiments were performed in normal and operated rats. Netrin-1 expression was not found in the optic nerve head of adult rats, normal and postlesion, but appeared transiently at 7 and 14 days after PN grafting. ISH signals of netrin receptors, however, disappeared from RGCs within 2 days after lesion and remained absent after PN grafting except for UNC5H2, which transiently occurred in a few RGCs. Netrin-1 expression was observed in the optic nerve head of adult fish, normal and postlesion, and the netrin-1 Fc fusion protein bound to young growing and all regenerating axons. Thus, the netrin-1-dependent guidance system continues to function in fish but apparently no longer operates in adult rats.
authors.affiliation: Department of Biology, University of Konstanz, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Petrausch
authors.forename: M
authors.initials: M
authors.lastname: Jung
authors.forename: C A
authors.initials: CA
authors.lastname: Leppert
authors.forename: C A
authors.initials: CA
authors.lastname: Stuermer
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology; D015536:Down-Regulation / Q000502:physiology; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D006054:Goldfish; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D009414:Nerve Growth Factors / Q000032:analysis / Q000235:genetics*; D009416:Nerve Regeneration / Q000502:physiology*; D000075386:Netrin Receptors; D000075388:Netrin-1; D009900:Optic Nerve / Q000166:cytology / Q000502:physiology / Q000637:transplantation*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D011956:Receptors, Cell Surface / Q000032:analysis / Q000235:genetics*; D012160:Retina / Q000166:cytology / Q000254:growth & development / Q000502:physiology; D012165:Retinal Ganglion Cells / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure; D025521:Tumor Suppressor Proteins
chemical_list: D009414:Nerve Growth Factors; D000075386:Netrin Receptors; C000617354:Ntn1 protein, rat; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D025521:Tumor Suppressor Proteins; D000075388:Netrin-1