title: The Saccharomyces cerevisiae YPR184w gene encodes the glycogen debranching enzyme.
abstract: The YPR184w gene encodes a 1536-amino acid protein that is 34-39% identical to the mammal, Drosophila melanogaster and Caenorhabditis elegans glycogen debranching enzyme. The N-terminal part of the protein possesses the four conserved sequences of the alpha-amylase superfamily, while the C-terminal part displays 50% similarity with the C-terminal of other eukaryotic glycogen debranching enzymes. Reliable measurement of alpha-1,4-glucanotransferase and alpha-1, 6-glucosidase activity of the yeast debranching enzyme was determined in strains overexpressing YPR184w. The alpha-1, 4-glucanotransferase activity of a partially purified preparation of debranching enzyme preferentially transferred maltosyl units than maltotriosyl. Deletion of YPR184w prevents glycogen degradation, whereas overexpression had no effect on the rate of glycogen breakdown. In response to stress and growth conditions, the transcriptional control of YPR184w gene, renamed GDB1 (for Glycogen DeBranching gene), is strictly identical to that of other genes involved in glycogen metabolism.
authors.affiliation: Centre de Bioingénierie Gilbert Durand, UMR-CNRS 5504, UR-INRA 792, Département de Génie Biochimique et Alimentaire, Complexe Scientifique de Rangueil, 31077 Cedex 04, Toulouse, France.
authors.forename: M A
authors.initials: MA
authors.lastname: Teste
authors.forename: B
authors.initials: B
authors.lastname: Enjalbert
authors.forename: J L
authors.initials: JL
authors.lastname: Parrou
authors.forename: J M
authors.initials: JM
authors.lastname: François
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017353:Gene Deletion; D015870:Gene Expression; D005800:Genes, Fungal; D005947:Glucose / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D006004:Glycogen Debranching Enzyme System / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D017386:Sequence Homology, Amino Acid
chemical_list: D006004:Glycogen Debranching Enzyme System; D011993:Recombinant Fusion Proteins; D006003:Glycogen; D005947:Glucose