title: Chloroplast development in Arabidopsis thaliana requires the nuclear-encoded transcription factor sigma B.
abstract: Development of plastids into chloroplasts, the organelles of photosynthesis, is triggered by light. However, little is known of the factors involved in the complex coordination of light-induced plastid gene expression, which must be directed by both nuclear and plastid genomes. We have isolated an Arabidopsis mutant, abc1, with impaired chloroplast development, which results in a pale green leaf phenotype. The mutated nuclear gene encodes a sigma factor, SigB, presumably for the eubacterial-like plastid RNA polymerase. Our results provide direct evidence that a nuclear-derived prokaryotic-like SigB protein, plays a critical role in the coordination of the two genomes for chloroplast development.
authors.affiliation: Mitsui Plant Biotechnology Research Institute, Tsukuba, Ibaraki, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Shirano
authors.forename: H
authors.initials: H
authors.lastname: Shimada
authors.forename: K
authors.initials: K
authors.lastname: Kanamaru
authors.forename: M
authors.initials: M
authors.lastname: Fujiwara
authors.forename: K
authors.initials: K
authors.lastname: Tanaka
authors.forename: H
authors.initials: H
authors.lastname: Takahashi
authors.forename: K
authors.initials: K
authors.lastname: Unno
authors.forename: S
authors.initials: S
authors.lastname: Sato
authors.forename: S
authors.initials: S
authors.lastname: Tabata
authors.forename: H
authors.initials: H
authors.lastname: Hayashi
authors.forename: C
authors.initials: C
authors.lastname: Miyake
authors.forename: A
authors.initials: A
authors.lastname: Yokota
authors.forename: D
authors.initials: D
authors.lastname: Shibata
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000648:ultrastructure*; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D002467:Cell Nucleus / Q000737:chemistry*; D002736:Chloroplasts / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D018744:DNA, Plant / Q000235:genetics; D005470:Fluorometry; D015870:Gene Expression; D008027:Light; D009154:Mutation; D010641:Phenotype; D018515:Plant Leaves / Q000254:growth & development; D018087:Plastids / Q000378:metabolism; D011994:Recombinant Proteins; D012808:Sigma Factor / Q000235:genetics / Q000502:physiology*; D014157:Transcription Factors
chemical_list: D001426:Bacterial Proteins; D018744:DNA, Plant; D011994:Recombinant Proteins; C079011:SigB protein, Bacteria; D012808:Sigma Factor; D014157:Transcription Factors