title: The role of genomics in approaching the study of Borrelia DNA replication.
abstract: The identification of chromosomal and episomal origins of replication in the genome of the causative agent of Lyme disease, the spirochete Borrelia burgdorferi, has been greatly facilitated by genomics. Analysis of genome features, including strand compositional asymmetries, organizational similarities to other bacterial origins of replication, and the presence of homologues of genes involved in replication and partitioning, have contributed to the identification of a collection of putative origins of replication within the Borrelia genome. This analysis has provided the basis for the experimental verification of origins in the linear chromosome and in the linear plasmid Ip28-2. Information generated during the study of these origins will significantly contribute to the understanding of the mechanisms of replication and partitioning in Borrelia.
authors.affiliation: Department of Microbiology, University of Georgia, Athens 30602, USA. jgarcial@panda.uchc.edu
authors.forename: J
authors.initials: J
authors.lastname: García-Lara
authors.forename: M
authors.initials: M
authors.lastname: Picardeau
authors.forename: B J
authors.initials: BJ
authors.lastname: Hinnebusch
authors.forename: W M
authors.initials: WM
authors.lastname: Huang
authors.forename: S
authors.initials: S
authors.lastname: Casjens
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D001898:Borrelia / Q000235:genetics*; D015748:Borrelia burgdorferi Group / Q000235:genetics / Q000472:pathogenicity; D004261:DNA Replication; D016680:Genome, Bacterial; D023281:Genomics / Q000379:methods; D006801:Humans; D008193:Lyme Disease / Q000382:microbiology; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins