STAT3 involvement in the acute phase-related expression of the rat haptoglobin gene.
Turpentine-induced acute-phase (AP) response in rats is followed by transcriptional activation of the haptoglobin (Hp) gene in liver. Analysing the promoter sequence of the rat Hp gene we postulated an involvement of Signal Transducer and Activator of Transcription 3 (STAT3) in the regulation of this process. Results obtained by using a combination of Western immunoblot and DNA-binding assays revealed AP-induced binding of constitutive 86kD-and inducible 91kD-STAT3 isoforms to the rat Hp gene inducible promoter element. On the basis of these data we assumed that AP-related interactions of these two STAT3 isoforms correlates with an activated transcriptional status of the rat Hp gene.
Grigorov
I
I
Institute for Biological Research, Department for Molecular Biology, Belgrade, Yugoslav Federal Republic.
Lazić
T
T
Cvetković
I
I
Milosavljević
T
T
Petrović
M
M
D000209:Acute-Phase Proteins / Q000235:genetics / Q000378:metabolism*; D000210:Acute-Phase Reaction / Q000139:chemically induced / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002457:Cell Extracts; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D006242:Haptoglobins / Q000235:genetics* / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D051381:Rats; D017208:Rats, Wistar; D050796:STAT3 Transcription Factor; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014425:Turpentine / Q000633:toxicity
D000209:Acute-Phase Proteins; D002457:Cell Extracts; D004268:DNA-Binding Proteins; D006242:Haptoglobins; D050796:STAT3 Transcription Factor; C494088:Stat3 protein, rat; D015534:Trans-Activators; D004247:DNA; D014425:Turpentine