Quantification of glycoalkaloids in tomato plants by time-resolved fluorescence using a europium chelator entrapped in liposomes.
In the control of tomato transgenic cultivars it is important to determine the glycoalkaloid content, which requires the screening of many samples. We have developed a simple method for the extraction and determination of glycoalkaloids in the leaf and fruit extracts of tomatoes using a europium chelator entrapped in phosphatidylcholine-cholesterol containing liposomes. The concentration values were quantified from liposome lysis effected by glycoalkaloid action. The fluorescent signal is linear between 1 and 10 micrograms of tomatine, which makes the method useful for the analysis of tomato samples.
Bacigalupo
M A
MA
Istituto di Biocatalisi e Riconoscimento Molecolare, CNR, Milan, Italy. bacigalupo@ico.mi.cnr.it
Ius
A
A
Longhi
R
R
Meroni
G
G
D002614:Chelating Agents; D002851:Chromatography, High Pressure Liquid; D005470:Fluorometry; D008081:Liposomes; D018551:Solanum lycopersicum / Q000737:chemistry*; D014053:Tomatine / Q000031:analogs & derivatives / Q000032:analysis* / Q000737:chemistry
D002614:Chelating Agents; D008081:Liposomes; D014053:Tomatine