title: Crystallization of the catalytic domain of murine terminal deoxynucleotidyl transferase.
abstract: The catalytic domain of murine terminal deoxynucleotidyl transferase (TdT) has been crystallized in the space group P2(1)2(1)2(1), with unit-cell parameters a = 47.1, b = 86.2, c = 111.7 A. The crystals diffract to a resolution of 2.4 A using synchrotron radiation and a full data set has been collected from the native crystals. The enzyme was shown to be active in the crystalline state.
authors.affiliation: Unité de Biochimie Structurale, Institut Pasteur, 25 Rue du Dr Roux, 75015 Paris, France.
authors.forename: N
authors.initials: N
authors.lastname: Sukumar
authors.forename: J B
authors.initials: JB
authors.lastname: Boulé
authors.forename: N
authors.initials: N
authors.lastname: Expert-Bezançon
authors.forename: N
authors.initials: N
authors.lastname: Jourdan
authors.forename: J
authors.initials: J
authors.lastname: Lescar
authors.forename: F
authors.initials: F
authors.lastname: Rougeon
authors.forename: C
authors.initials: C
authors.lastname: Papanicolaou
authors.forename: M
authors.initials: M
authors.lastname: Delarue
mesh_terms: D000818:Animals; D001665:Binding Sites; D002384:Catalysis; D018360:Crystallography, X-Ray; D004253:DNA Nucleotidylexotransferase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D051379:Mice; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D011994:Recombinant Proteins; D004253:DNA Nucleotidylexotransferase