Projections and neurochemistry of interneurones in the myenteric plexus of the guinea-pig gastric corpus.
Recently, motor neurones of the myenteric plexus innervating the muscle layers or the mucosa have been identified in the guinea-pig stomach. We applied the neuronal tracer DiI (1,1'-didodecyl-3,3,3', 3'-tetramethylindocarbocyanine perchlorat) onto myenteric ganglia in order to identify populations of interneurones in the myenteric plexus of the guinea-pig stomach. The tracing was combined with the immunohistochemical detection of calbindinD28k (CALB), choline acetyltransferase (ChAT), neuropeptide Y (NPY) and 5-hydroxytryptamine (serotonin) (5-HT) and the results were compared to the neurochemical coding of target specific motor neurones. Long projecting ( approximately 5.4 mm) ChAT/CALB/+/-5-HT-, nitric oxide synthase (NOS)/CALB- and short projecting ( approximately 1.1 mm) ChAT/NPY-neurones were identified as descending interneurones. CALB positive ascending interneurones contained ChAT but rarely 5-HT (code: ChAT/CALB). This study identified ascending and descending interneurones in the gastric myenteric plexus and revealed the neurochemical coding of some of the interneurone populations.
Reiche
D
D
Department of Physiology, School of Veterinary Medicine, Bischofsholer Damm 15, D-30173, Hannover, Germany.
Michel
K
K
Pfannkuche
H
H
Schemann
M
M
D000818:Animals; D001369:Axons / Q000378:metabolism / Q000648:ultrastructure; D064026:Calbindins; D002232:Carbocyanines; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D005260:Female; D005456:Fluorescent Dyes; D005753:Gastric Mucosa / Q000378:metabolism; D006168:Guinea Pigs; D007395:Interneurons / Q000166:cytology / Q000378:metabolism*; D008297:Male; D009046:Motor Neurons / Q000166:cytology / Q000378:metabolism; D009197:Myenteric Plexus / Q000166:cytology / Q000378:metabolism*; D009434:Neural Pathways / Q000166:cytology / Q000378:metabolism*; D009478:Neuropeptide Y / Q000378:metabolism; D018377:Neurotransmitter Agents / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D064030:S100 Calcium Binding Protein G / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D013270:Stomach / Q000166:cytology / Q000294:innervation*
C035005:3,3'-dihexadecylindocarbocyanine; D064026:Calbindins; D002232:Carbocyanines; D005456:Fluorescent Dyes; D009478:Neuropeptide Y; D018377:Neurotransmitter Agents; D064030:S100 Calcium Binding Protein G; D012701:Serotonin; D019001:Nitric Oxide Synthase; D002795:Choline O-Acetyltransferase