title: Genotyping of clinical and chicken isolates of Campylobacter jejuni and Campylobacter coli.
abstract: Genomic DNA from 58 strains of Campylobacter made up of 48 Campylobacter jejuni and ten Campylobacter coli were digested with Sma I and analysed by pulsed-field gel electrophoresis (PFGE). The cleavage of DNA by Sma I gave 22 distinct hybridization patterns. The two Campylobacter species were subtyped by PFGE. The average genomic size for C. jejuni by Sma I digestion was 1.73 Mb, while that of C. coli gave 1.7 Mb. Results from this study indicate that PFGE analysis by Sma I digested genomic DNA provides a reliable means of differentiating between and within species of Campylobacter and provides a practical approach to epidemiological studies of Campylobacter.
authors.affiliation: Medical Department 1, Technical University Hospital, Dresden, Germany.
authors.forename: S I
authors.initials: SI
authors.lastname: Smith
authors.forename: D K
authors.initials: DK
authors.lastname: Olukoya
authors.forename: A J
authors.initials: AJ
authors.lastname: Fox
authors.forename: A O
authors.initials: AO
authors.lastname: Coker
mesh_terms: D000818:Animals; D002169:Campylobacter Infections / Q000235:genetics*; D017000:Campylobacter coli / Q000235:genetics*; D016123:Campylobacter jejuni / Q000235:genetics*; D002645:Chickens / Q000235:genetics / Q000382:microbiology*; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006801:Humans; D015183:Restriction Mapping; D012703:Serotyping / Q000706:statistics & numerical data