Characterization and sequence elucidation of a novel peptide with molt-inhibiting activity from the South African spiny lobster, Jasus lalandii.
We have isolated a peptide from extracts of sinus glands from a South African spiny lobster species, Jasus lalandii, by high-performance liquid chromatography (HPLC) and identified it as a putative molt-inhibiting hormone (MIH) by (i) an in vitro assay with J. lalandii Y-organs to measure the inhibition of ecdysteroid synthesis and (ii) an immunoassay using antiserum raised against MIH of the edible crab. The MIH of J. lalandii has 74 amino acid residues, a molecular mass of 9006 Da, a free N-terminus and an amidated C-terminus. The full primary sequence has been obtained from sequencing various digest fragments (tryptic, endoproteinase Asp-N, cyanogen bromide) of the unreduced (native) peptide: RFTFDCPGMMGQRYLYEQVEQVCDDCYNLYREEKIAVNCRENCFLNSWFTVCLQATMREHETPRFDIWR SIILKA-NH(2). Structural comparisons with other peptides show that the J. lalandii MIH belongs to the peptide family which includes the crustacean hyperglycemic hormone, molt-inhibiting hormone and vitellogenesis-inhibiting hormone (cHH/MIH/VIH). This novel peptide has 36-43% sequence identity to putative MIHs from other decapod crustaceans and 32-34% identity to the two cHH peptides previously identified in this spiny lobster species. This is the first report of a peptide with MIH activity in the Palinuridae infraorder.
Marco
H G
HG
Department of Zoology, University of Cape Town, 7701, Rondebosch, South Africa.
Stoeva
S
S
Voelter
W
W
Gäde
G
G
D000595:Amino Acid Sequence; D000818:Animals; D001681:Biological Assay; D003445:Crustacea / Q000502:physiology*; D003488:Cyanogen Bromide; D006943:Hyperglycemia / Q000139:chemically induced; D007447:Invertebrate Hormones / Q000302:isolation & purification* / Q000494:pharmacology; D008969:Molecular Sequence Data; D018978:Molting / Q000502:physiology*; D009479:Neuropeptides / Q000302:isolation & purification* / Q000494:pharmacology; D010446:Peptide Fragments / Q000302:isolation & purification; D013019:South Africa; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D007447:Invertebrate Hormones; D009479:Neuropeptides; D010446:Peptide Fragments; C012445:molt-inhibiting hormone; D003488:Cyanogen Bromide