Identification of epitopes for cross-reaction, auto-reaction and autoantibodies to catalase.
We identified in bovine and rat catalase two epitopes that were responsible for a cross-reaction between the two species using cross-reactive antibodies raised in rabbits by immunization with their respective catalases. The epitopes were(444)TFYLK(448)and(458)LCENIAGHLK(467)in bovine catalase and(445)TFYTK(449)and(459)LCENIANHLK(468)in rat catalase. The two epitopes in rats were also identified as epitopes for auto-reactive antibodies that were raised in rats by immunization with bovine catalase and were reactive with a homologous rat catalase. Furthermore, one of the rat epitopes,(459)LCENIANHLK(468), was identified as the epitope for autoantibodies which were inducible by intensive immunization in rats with the homologous rat catalase. A possible implication of the epitopes in certain human diseases is discussed.
Miura
H
H
Tumour Laboratory, The Foundation for Basic Research of Oncology, Tokyo, Kokubunji, Japan. tulafbro@pc.highway.ne.jp
Tobe
T
T
Miura
K
K
Kobayashi
K
K
Higashi
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001323:Autoantibodies / Q000032:analysis* / Q000276:immunology; D002374:Catalase / Q000276:immunology*; D002417:Cattle; D003429:Cross Reactions; D000939:Epitopes; D007106:Immune Sera / Q000276:immunology; D007114:Immunization; D008969:Molecular Sequence Data; D011817:Rabbits; D051381:Rats
D001323:Autoantibodies; D000939:Epitopes; D007106:Immune Sera; D002374:Catalase