title: Establishment of human pancreatic ductal cells in a long-term culture.
abstract: Cultivation and preservation of human pancreatic ductal cells have remained a challenge. With a defined culture medium and refinement of culturing techniques, we have been able to maintain human pancreatic ductal cells without any genetic manipulation in culture for more than 16 months. Freshly isolated ductal fragments were placed on a rocker in M3:5 medium free of collagen for 14 days to remove fibroblasts and endocrine cells before allowing them to attach. The cells produced an excessive amount of mucin and expressed the duct specific cytokeratins (CK) 7 and 19, DU-PAN2, CA19-9, carbonic anhydrase II (CA II), and secretin receptors. During the course of the culture, however, the cells gradually lost the expression of CA II, secretin receptors, DU-PAN2, and CA 19-9 and assumed an undifferentiated phenotype, which showed an upregulation of transforming growth factor alpha (TGFalpha) and epidermal growth factor receptor (EGFR), an increase in the expression of Ki-67, and an increased binding to Phaseolus vulgaris leucoagglutinin (PHA-L) and tomato lectin. These ductal cells present a useful source with which to study physiologic aspects of ductal cells including differentiation.
authors.affiliation: UNMC Eppley Cancer Center, Omaha 68198-6805, USA.
authors.forename: A B
authors.initials: AB
authors.lastname: Ulrich
authors.forename: B M
authors.initials: BM
authors.lastname: Schmied
authors.forename: H
authors.initials: H
authors.lastname: Matsuzaki
authors.forename: T
authors.initials: T
authors.lastname: El-Metwally
authors.forename: M P
authors.initials: MP
authors.lastname: Moyer
authors.forename: C
authors.initials: C
authors.lastname: Ricordi
authors.forename: T E
authors.initials: TE
authors.lastname: Adrian
authors.forename: S K
authors.initials: SK
authors.lastname: Batra
authors.forename: P M
authors.initials: PM
authors.lastname: Pour
mesh_terms: D000328:Adult; D002448:Cell Adhesion; D002453:Cell Cycle; D002455:Cell Division; D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000378:metabolism; D006801:Humans; D007633:Keratins / Q000032:analysis; D008297:Male; D010183:Pancreatic Ducts / Q000737:chemistry / Q000166:cytology*; D012633:Secretin / Q000378:metabolism; D014746:Vimentin / Q000032:analysis
chemical_list: D014746:Vimentin; D012633:Secretin; D004815:Epidermal Growth Factor; D007633:Keratins