title: Human and rat mononuclear cell proliferation show different sensitivity, in vitro, to single constituents of dental composite resins.
abstract: The aim of this study was to evaluate the effect of 11 constituents of dental composite resins on ConA-induced proliferation of rat mononuclear cells. We also wanted to compare the sensitivity of rat and human mononuclear cells, to find out if results from a rat cell assay could be extrapolated to human conditions. The variable used for this purpose was the dose at 50% inhibition of proliferation (ID(50)). Mononuclear cells derived from rat spleens and human venous blood were used. The effects of the substances on rat and human cell proliferation were evaluated with and without stimulation by Concanavalin A. The ID(50) rank order of the tested substance categories was similar for both species, i.e., the monomers inhibited at the lowest concentration, the stabilizer at intermediate, and the initiators at the highest concentrations. In spite of a similar inhibition rank order, there were significant differences in sensitivity to the substances between the species. In conclusion, the results from this study together with the fact that the substances are handled by and used in humans, would favor the use of human cells when studying the effect of constituents of dental composite resins in vitro.
authors.affiliation: Department of Prosthetic Dentistry / Dental Materials Science, Faculty of Odontology, Göteborg University, Göteborg, Sweden. Gunilla.Aronsson@odontologi.gu.se
authors.forename: G
authors.initials: G
authors.lastname: Aronsson
authors.forename: U I
authors.initials: UI
authors.lastname: Dahlgren
authors.forename: S
authors.initials: S
authors.lastname: Karlsson
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local / Q000494:pharmacology; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D003188:Composite Resins / Q000494:pharmacology*; D003208:Concanavalin A / Q000494:pharmacology; D003764:Dental Materials / Q000494:pharmacology*; D000431:Ethanol / Q000494:pharmacology; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008970:Molecular Weight; D009000:Monocytes / Q000187:drug effects*; D051381:Rats; D011917:Rats, Inbred Lew; D013045:Species Specificity; D013154:Spleen / Q000166:cytology / Q000187:drug effects
chemical_list: D000891:Anti-Infective Agents, Local; D003188:Composite Resins; D003764:Dental Materials; D003208:Concanavalin A; D000431:Ethanol