title: What you should know about PR3-ANCA. Structural aspects of antibodies to proteinase 3 (PR3).
abstract: Reactive antigenic epitopes on presumed autoantigens of biologic interest have been examined by many researchers. The central third complementarity-determining region (CDR3) residues of a human monoclonal anti-proteinase 3 (PR3) antibody contained many negatively charged aspartic acid residues, perhaps contributing to its reactivity with positively charged PR3 regions. Examination of four other human monoclonal anti-PR3 antibodies shows a number of negatively charged residues within their CDR3 regions. Mapping of segments of linear PR3-epitopes reacting with anti-neutrophil cytoplasmic antibodies (ANCA) demonstrated a preliminary estimate of structures contributing to antigenic determinants. T-cell epitopes on PR3 are reported in studies of chronic myeloid leukemia. These T-cell epitopes appear to be human leukocyte antigen (HLA) A2.1 restricted.
authors.affiliation: Department of Medicine, University of Bergen, Haukeland Hospital, Bergen, Norway. elisabeth.peen@medb.uib.no
authors.forename: E
authors.initials: E
authors.lastname: Peen
authors.forename: R C
authors.initials: RC
authors.lastname: Williams
mesh_terms: D000818:Animals; D019268:Antibodies, Antineutrophil Cytoplasmic / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D006801:Humans; D053146:Myeloblastin; D011994:Recombinant Proteins / Q000276:immunology; D012697:Serine Endopeptidases / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D019268:Antibodies, Antineutrophil Cytoplasmic; D000939:Epitopes; D011994:Recombinant Proteins; D012697:Serine Endopeptidases; D053146:Myeloblastin