Birth of a piglet derived from an oocyte fertilized by intracytoplasmic sperm injection (ICSI).
This report is of a live piglet derived from an embryo produced by intracytoplasmic sperm injection and carried to term. In vivo-matured oocytes were injected with in vitro-capacitated spermatozoa and after 40 h of culture, 2 to 4-cell-stage embryos were transferred to the oviducts of oestrus induced gilts 24 h after the expected ovulation time. Pregnancy of the surrogate mothers was maintained by estrogen treatment on days 10 to 16 after transfer. The single piglet that was born to a mother that had received 32 embryos was underweight, though healthy and lively.
Kolbe
T
T
Institute of Animal Husbandry and Genetics, University of Göttingen, Albrecht-Thaer-Weg 3, 37075, Göttingen, Germany.
Holtz
W
W
D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D006063:Chorionic Gonadotropin / Q000008:administration & dosage; D004624:Embryo Transfer / Q000662:veterinary; D004958:Estradiol / Q000008:administration & dosage; D005260:Female; D007476:Ionophores / Q000737:chemistry; D008297:Male; D009865:Oocytes / Q000502:physiology*; D011247:Pregnancy; D020554:Sperm Injections, Intracytoplasmic / Q000662:veterinary*; D013552:Swine / Q000502:physiology*
D006063:Chorionic Gonadotropin; D007476:Ionophores; D004958:Estradiol