Transgene and transposon silencing in Chlamydomonas reinhardtii by a DEAH-box RNA helicase.
The molecular mechanism(s) responsible for posttranscriptional gene silencing and RNA interference remain poorly understood. We have cloned a gene (Mut6) from the unicellular green alga Chlamydomonas reinhardtii that is required for the silencing of a transgene and two transposon families. Mut6 encodes a protein that is highly homologous to RNA helicases of the DEAH-box family. This protein is necessary for the degradation of certain aberrant RNAs, such as improperly processed transcripts, which are often produced by transposons and some transgenes.
Wu-Scharf
D
D
School of Biological Sciences and Plant Science Initiative, University of Nebraska-Lincoln, E211 Beadle Center, Post Office Box 880666, Lincoln, NE 68588, USA.
Jeong
B
B
Zhang
C
C
Cerutti
H
H
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D016825:Chlamydomonas reinhardtii / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D020868:Gene Silencing; D006801:Humans; D008969:Molecular Sequence Data; D012313:RNA / Q000378:metabolism; D020365:RNA Helicases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D018626:Retroelements; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D019076:Transgenes
D004251:DNA Transposable Elements; D012333:RNA, Messenger; D018626:Retroelements; D012313:RNA; D020365:RNA Helicases