title: Effects of macromolecular growth substrates on production of extracellular enzymes by Bacillus species in continuous culture.
abstract: Bacillus species 11089 and alkalophilic Bacillus species 11203 were both capable of growth in continuous culture on macromolecular substrates, starch, soybean flour, casein, pectin, polypectate, polygalacturonate and carboxymethylcellulose (CMC) when these were used as the carbon-energy source in a mineral salts basal medium. High maximum specific growth rate (micronmax) and biomass values occurred when cells were grown on starch, soybean flour and casein, and low values on pectin, polypectate, polygalacturonic acid and CMC. Hydrolytic enzymes (protease, amylase, polygalacturonate lyase and alkaline phosphatase) were subject to regulation (induction and/or repression) depending on the nature of the growth substrate utilized. In general, high levels of enzymes were produced on soybean flour, casein and starch but low levels on CMC, pectin, polypectate and polygalacturonate.
authors.affiliation: Faculty of Applied Sciences, University of the West of England, Bristol, Great Britain.
authors.forename: A U
authors.initials: AU
authors.lastname: Mahmood
authors.forename: J
authors.initials: J
authors.lastname: Greenman
authors.forename: A H
authors.initials: AH
authors.lastname: Scragg
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001407:Bacillus / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D001412:Bacillus subtilis / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D006133:Growth Substances / Q000378:metabolism; D046911:Macromolecular Substances; D012697:Serine Endopeptidases / Q000378:metabolism; D000516:alpha-Amylases / Q000378:metabolism
chemical_list: D003470:Culture Media; D006133:Growth Substances; D046911:Macromolecular Substances; D000469:Alkaline Phosphatase; D000516:alpha-Amylases; D012697:Serine Endopeptidases; C032923:microbial serine proteinases