[Analysis of isogenic lines of the soft wheat carrying dominant alleles of Bg, Hg, and Rg1 genes using microsatellite and protein markers].
To determine tight linkage between morphological and molecular markers of the first homologous group of chromosomes of common wheat, microsatellite analysis of six near-isogenic lines with marker dominant alleles controlling back color (Bg; 1AS) and hairy glume (Hg; 1AS) and two lines bearing the dominant alleles of the gene for red glume color (Rgl; 1BS) was conducted. The component composition of gliadins in these lines was studied. Tight linkage of Bg, Hg, and Gli-A1 genes with a microsatellite marker Xgwm136 (1AS) and of Rg1 and Gli-B1 genes with markers Xgwm33 and Xgwm550 (1BS) was shown. Based on the results obtained and literature data, the most probable order of morphological and molecular markers on chromosomes of common wheat was determined. On chromosome 1AS, from the centromere to the telomere, the markers are located as follows: Xgwm136-Gli-A1-BgHg; on chromosome 1BS, in the same direction: Xgwm33-Gli-B1-Rg1-Xgwm550.
Khlëstkina
E K
EK
Institute of Cytology and Genetics, Russian Academy of Sciences, Novosibirsk, Russia. khlest@bionet.nsc.ru
Salina
E A
EA
Pshenichnikova
T A
TA
Arbuzova
V S
VS
Koval'
S F
SF
D002874:Chromosome Mapping; D005799:Genes, Dominant; D017343:Genes, Plant; D005819:Genetic Markers; D005903:Gliadin / Q000737:chemistry; D018895:Microsatellite Repeats; D010860:Pigments, Biological / Q000235:genetics; D016133:Polymerase Chain Reaction; D014908:Triticum / Q000235:genetics* / Q000502:physiology
D005819:Genetic Markers; D010860:Pigments, Biological; D005903:Gliadin