title: A molecular pathway for light-dependent photoreceptor apoptosis in Drosophila.
abstract: Light-induced photoreceptor apoptosis occurs in many forms of inherited retinal degeneration resulting in blindness in both vertebrates and invertebrates. Though mutations in several photoreceptor signaling proteins have been implicated in triggering this process, the molecular events relating light activation of rhodopsin to photoreceptor death are yet unclear. Here, we uncover a pathway by which activation of rhodopsin in Drosophila mediates apoptosis through a G protein-independent mechanism. This process involves the formation of membrane complexes of phosphorylated, activated rhodopsin and its inhibitory protein arrestin, and subsequent clathrin-dependent endocytosis of these complexes into a cytoplasmic compartment. Together, these data define the proapoptotic molecules in Drosophila photoreceptors and indicate a novel signaling pathway for light-activated rhodopsin molecules in control of photoreceptor viability.
authors.affiliation: Department of Pharmacology, UT Southwestern Medical Center, Dallas, Texas 75390, USA.
authors.forename: A
authors.initials: A
authors.lastname: Kiselev
authors.forename: M
authors.initials: M
authors.lastname: Socolich
authors.forename: J
authors.initials: J
authors.lastname: Vinós
authors.forename: R W
authors.initials: RW
authors.lastname: Hardy
authors.forename: C S
authors.initials: CS
authors.lastname: Zuker
authors.forename: R
authors.initials: R
authors.lastname: Ranganathan
mesh_terms: D000818:Animals; D017209:Apoptosis; D019390:Arrestins / Q000378:metabolism; D001667:Binding, Competitive / Q000235:genetics; D002135:Calcium-Binding Proteins; D002966:Clathrin / Q000378:metabolism; D004330:Drosophila / Q000378:metabolism*; D029721:Drosophila Proteins; D004705:Endocytosis; D005786:Gene Expression Regulation; D008027:Light / Q000009:adverse effects*; D009154:Mutation; D010749:Phosphoprotein Phosphatases / Q000235:genetics; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D017956:Photoreceptor Cells, Invertebrate / Q000378:metabolism* / Q000473:pathology; D012162:Retinal Degeneration / Q000378:metabolism*; D012243:Rhodopsin / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: D019390:Arrestins; D002135:Calcium-Binding Proteins; D002966:Clathrin; D029721:Drosophila Proteins; D010750:Phosphoproteins; C013110:metarhodopsins; D012243:Rhodopsin; D010749:Phosphoprotein Phosphatases; C074643:rdgC protein, Drosophila