[Prionization of the Pichia methanolica SUP35 gene product in the yeast Saccharomyces cerevisiae].
Induction of the prionlike form of the SUP35 gene of Pichia methanolica, the [PSIP+] factor, was shown in the transgenic yeast Saccharomyces cerevisiae containing the P. methanolica SUP35 gene located in the chromosome instead of the indigenous SUP35 gene. Either the induction of the [PSIP+] factor in the transgenic yeast, unlike that of the classical [PSI+] factor, does not depend on the presence of the [PIN+] determinant in the cell or the substitution of the S. cerevisiae SUP35 gene for the P. methanolica SUP35 gene changes the PIN status of the strain. The [PSIP+] factor is unstable in mitosis and meiosis and is not effectively eliminated upon over-production of the chaperone protein Hsp104p of S. cerevisiae. The existence of an interspecific barrier during transmission of the prionlike state from S. cerevisiae Sup35p to P. methanolica Sup35p was shown.
Zadorskiĭ
S P
SP
Department of Genetics and Breeding, St. Petersburg State University, Russia.
Sopova
Iu V
IuV
Inge-Vechtomov
S G
SG
D015825:Chromosomes, Fungal / Q000235:genetics; D018389:Codon, Nonsense; D004171:Diploidy; D005656:Fungal Proteins / Q000235:genetics*; D016153:Genes, Suppressor; D006238:Haploidy; D010454:Peptide Termination Factors / Q000235:genetics*; D010843:Pichia / Q000235:genetics*; D011328:Prions / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D019076:Transgenes
D018389:Codon, Nonsense; D005656:Fungal Proteins; D010454:Peptide Termination Factors; D011328:Prions; C068068:SUP35 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C087971:peptide-chain-release factor 3