title: The beta-actin gene of two species of southern top mouth minnow (Pseudorasbora parva) and the common fat minnow (Rhynchocypris oxycephalus) from the family Cyprinidae.
abstract: The beta-actin genes of two species of southern top mouth minnow and the common fat minnow from the family Cyprinidae were amplified and characterized. The exon and intron boundaries of the beta-actin gene matched the consensus acceptor and donor sites according to the GT/AG rule. The amino acid sequence identity between the two species is 100.0% while there is a lower degree of homology with other species in the range of 98.7% to 100.0%. The expression of the beta-actin gene was confirmed in liver.
authors.affiliation: Environmental Health Sciences Division, National Institute for Environmental Studies, Tsukuba, Ibaraki, Japan. jae-seong.lee@nies.go.jp
authors.forename: J S
authors.initials: JS
authors.lastname: Lee
authors.forename: S H
authors.initials: SH
authors.lastname: Lee
authors.forename: M C
authors.initials: MC
authors.lastname: Gye
mesh_terms: D000199:Actins / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003530:Cyprinidae / Q000235:genetics*; D006801:Humans; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D000199:Actins