title: Identification of a K(d)-restricted antigenic peptide encoded by murine cytomegalovirus early gene M84.
abstract: The two sister cytomegaloviruses (CMVs), human and murine CMV, have both evolved immune evasion functions that interfere with the major histocompatibility complex class I (MHC-I) pathway of antigen processing and presentation and are effectual in the early (E) phase of virus gene expression. However, studies on murine CMV have shown that E-phase immune evasion is leaky. An E-phase protein involved in immune evasion, namely m04-gp34, was found to simultaneously account for an antigenic peptide presented by the MHC-I molecule D(d). Recent work has demonstrated the induction of protective immunity specific for the E-phase protein M84-p65, one of two murine CMV homologues of the human CMV matrix protein UL83-pp65. In this study, the identification of the MHC-I K(d)-restricted M84 peptide (297)AYAGLFTPL(305) is documented. This peptide is the third antigenic peptide described for murine CMV and the second that escapes immunosubversive mechanisms.
authors.affiliation: Institute for Virology, Johannes Gutenberg University, Hochhaus am Augustusplatz, 55101 Mainz, Germany1.
authors.forename: Rafaela
authors.initials: R
authors.lastname: Holtappels
authors.affiliation: Institute for Virology, Johannes Gutenberg University, Hochhaus am Augustusplatz, 55101 Mainz, Germany1.
authors.forename: Doris
authors.initials: D
authors.lastname: Thomas
authors.affiliation: Institute for Virology, Johannes Gutenberg University, Hochhaus am Augustusplatz, 55101 Mainz, Germany1.
authors.forename: Matthias J
authors.initials: MJ
authors.lastname: Reddehase
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000737:chemistry / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D017781:Genes, Immediate-Early / Q000235:genetics*; D006183:H-2 Antigens / Q000276:immunology*; D017874:Immediate-Early Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D007156:Immunologic Memory / Q000276:immunology; D018655:Lymphocyte Count; D051379:Mice; D008807:Mice, Inbred BALB C; D018146:Muromegalovirus / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D016366:Open Reading Frames / Q000235:genetics; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
chemical_list: D000956:Antigens, Viral; D006183:H-2 Antigens; D017874:Immediate-Early Proteins; D010446:Peptide Fragments; C044582:immediate-early proteins, cytomegalovirus