Relative neutralizing activity in polyspecific IgM, IgA, and IgG preparations against group A streptococcal superantigens.
In this study we compared the ability of different immunoglobulin (Ig) preparations containing IgG, IgM, and/or IgA to neutralize the activity of streptococcal pyrogenic exotoxin A (SpeA) or culture supernatant from a clinical group A streptococcal isolate. All Ig preparations markedly inhibited the mitogenic and cytokine-inducing activity of SpeA and culture supernatant at concentrations of 0.05-0.5 mg/mL, and at 0.5 mg/mL, most caused 95-100% inhibition of both stimuli. A significantly higher (P< or =.05) inhibition of SpeA was achieved by Pentaglobin (IgG, IgM, and IgA) and IgAbulin (IgA and IgG), as compared with pure IgG preparations. IgM- and IgA-enriched preparations had significantly higher inhibitory activity against SpeA than against culture supernatant, whereas the reverse was true for the IgG preparations (P< or =.05). The data show that IgM and IgA are potent inhibitors of specific streptococcal superantigens. These findings may have implications for the optimization of immunotherapy in invasive streptococcal infections.
Norrby-Teglund
A
A
Division of Infectious Diseases, Huddinge University Hospital, Huddinge, Sweden. Anna.Norrby-Teglund@medhs.ki.se
Ihendyane
N
N
Kansal
R
R
Basma
H
H
Kotb
M
M
Andersson
J
J
Hammarström
L
L
D017077:Culture Media, Conditioned; D016207:Cytokines / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology / Q000494:pharmacology; D007074:Immunoglobulin G / Q000276:immunology / Q000494:pharmacology; D007075:Immunoglobulin M / Q000276:immunology / Q000494:pharmacology; D007136:Immunoglobulins / Q000276:immunology* / Q000494:pharmacology; D016756:Immunoglobulins, Intravenous / Q000276:immunology / Q000494:pharmacology; D007371:Interferon-gamma / Q000378:metabolism; D007375:Interleukin-1 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008233:Lymphotoxin-alpha / Q000378:metabolism; D009500:Neutralization Tests; D013297:Streptococcus pyogenes / Q000276:immunology*; D018089:Superantigens / Q000276:immunology* / Q000494:pharmacology
D017077:Culture Media, Conditioned; D016207:Cytokines; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D016756:Immunoglobulins, Intravenous; D007375:Interleukin-1; D008233:Lymphotoxin-alpha; D018089:Superantigens; D007371:Interferon-gamma