Wound healing acceleration of a novel transforming growth factor-beta inducer, SEK-1005.
The studies were carried out to elucidate the effect of a novel cyclic peptide, SEK-1005 (C(45)H(70)N(8)O(13)), on wound healing. SEK-1005 (4-10 microg/wound) applied topically significantly accelerated the healing of a full-thickness wound on the dorsal skin of a rat. In a healing-impaired mouse, the peptide (2-10 microg/wound) had more potent activity, exerting an effect comparable to that of basic fibroblast growth factor (FGF). However, SEK-1005 (0.1-100 ng/ml) scarcely promoted the proliferation of cultured fibroblasts (NIH3T3 cells) while basic FGF (0.2-5 ng/ml) showed marked mitogenic activity. SEK-1005 (2-10 microg/wound) significantly increased the topical production of transforming growth factor (TGF)-beta1, a cytokine that is known to accelerate wound healing. This activity was closely correlated with the wound-repairing effect. From the above, SEK-1005 can be considered as a new type of wound healing agent with potent TGF-beta1-inducing activity.
Abe
Y
Y
Medical Research Laboratory, Sekisui Chemical Co., LTD., 2-1 Hyakuyama Shimamoto-cho, Mishima-gun, 618-0021, Osaka, Japan. abe08@smile.sekisui.co.jp
Inagaki
K
K
Fujiwara
A
A
Kuriyama
K
K
D016475:3T3 Cells / Q000187:drug effects; D000818:Animals; D001426:Bacterial Proteins / Q000494:pharmacology*; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010456:Peptides, Cyclic / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000187:drug effects / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000187:drug effects* / Q000378:metabolism; D053773:Transforming Growth Factor beta1; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
D001426:Bacterial Proteins; D010456:Peptides, Cyclic; C407521:SEK 1005; C508961:Tgfb1 protein, mouse; C508962:Tgfb1 protein, rat; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1