Bone tissue composition, dimensions and strength in female rats given an increased dietary level of vitamin A or exposed to 3,3',4, 4',5-pentachlorobiphenyl (PCB126) alone or in combination with vitamin C.
In previous studies we have described structural and functional changes in rat bone tissue caused by 3,3',4,4',5-pentachlorobiphenyl (PCB126). Some of the effects caused by PCB126 resemble those found in vitamin C-deficient rats, as well as those found in rats with a high dietary intake of vitamin A. The present investigation was designed to determine if these PCB126-induced changes could be inhibited by addition of vitamin C to the drinking water and if they could be evoked by vitamin A administration. Five groups of female rats were used in this study, which lasted for 12 weeks. Three of the groups were exposed to PCB126 (total dose 320 microgram/kg, bw), either alone or in combination with vitamin C added to the drinking water (1 and 10 g/l, respectively). One group was given feed with increased level of vitamin A (600000 U/kg pellet) and the fifth group served as controls. Using peripheral quantitative computed tomography (pQCT), it was found that PCB126 increased trabecular density and cortical thickness, but reduced the trabecular area. Furthermore, maximum torque and stiffness of the humerus during torsional testing and serum osteocalcin levels were reduced by PCB126. Of the PCB126 induced effects observed, addition of vitamin C only inhibited the reduction of serum osteocalcin. Like PCB126 vitamin A supplementation increased the inorganic content and the bone density and also reduced the trabecular area and polar moment of inertia but did not increase the cortical thickness or reduce maximum torque, stiffness or serum osteocalcin level. Apparently, the effects induced by PCB126 are not mediated either via decreased vitamin C level or increased vitamin A level.
Lind
P M
PM
Uppsala University, Department of Environmental Toxicology, Norbyvägen 18A, 752 36, Uppsala, Sweden. monica.lind@ebc.uu.se
Larsson
S
S
Johansson
S
S
Melhus
H
H
Wikström
M
M
Lindhe
O
O
Orberg
J
J
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D001842:Bone and Bones / Q000033:anatomy & histology / Q000187:drug effects* / Q000378:metabolism; D018483:Diaphyses / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism; D004032:Diet; D004838:Epiphyses / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism; D004965:Estrogen Antagonists / Q000633:toxicity*; D005260:Female; D006811:Humerus / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D015675:Osteocalcin / Q000494:pharmacology; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014057:Tomography, X-Ray Computed; D014801:Vitamin A / Q000494:pharmacology*
D004965:Estrogen Antagonists; D015675:Osteocalcin; D014801:Vitamin A; D011078:Polychlorinated Biphenyls; D001205:Ascorbic Acid; C023035:3,4,5,3',4'-pentachlorobiphenyl