Inhibition of energy production in vitro by glutaric acid in cerebral cortex of young rats.
The present study investigated the effects of glutaric acid (GA), which predominantly accumulates in glutaric acidemia type I (GA-I), on some in vitro parameters of energy metabolism in cerebral cortex of rats. We first evaluated CO2 production from [U-14C] acetate, as well as ATP levels in brain of young Wistar rats. The effect of the acid on the activities of the respiratory chain complexes were also investigated. GA was tested at final concentrations ranging from 0.5 to 5.0 mM. GA significantly reduced brain CO2 production by 50% at the concentrations of 0.5 to 3.0 mM, ATP levels by 25% at the concentration of 3.0 mM, succinate:cytochrome C oxireductase (complex II plus CoQ plus complex III) by 25% at 5 mM concentration, and NADH:cytochrome C oxireductase (complex I plus CoQ plus complex Ill) by 25% at 2.5 and 5 mM concentrations. The results strongly indicate that GA impairs brain energy production. If these effects also occur in humans, it is possible that they may contribute to the neuropathology of patients affected by GA-I.
Silva
C G
CG
Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brasil.
Silva
A R
AR
Ruschel
C
C
Helegda
C
C
Wyse
A T
AT
Wannmacher
C M
CM
Dutra-Filho
C S
CS
Wajner
M
M
D000085:Acetates / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000592:Amino Acid Metabolism, Inborn Errors / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology*; D000818:Animals; D000831:Animals, Newborn; D002245:Carbon Dioxide / Q000378:metabolism; D002250:Carbon Radioisotopes; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004579:Electron Transport / Q000187:drug effects / Q000502:physiology; D004734:Energy Metabolism / Q000187:drug effects* / Q000502:physiology; D005977:Glutarates / Q000633:toxicity*; D066298:In Vitro Techniques; D051381:Rats; D017208:Rats, Wistar
D000085:Acetates; D002250:Carbon Radioisotopes; D005977:Glutarates; D002245:Carbon Dioxide; D000255:Adenosine Triphosphate; C035736:glutaric acid