title: Acid-base analysis during experimental anemia in rats.
abstract: The present study evaluated the acid-base status of anemic rats by using two approaches of acid-base analysis: one based on the base excess (BE) calculation and the other based on Stewart's physicochemical analysis. Two sets of experimental data, derived from two different methods of inducing anemia, were used: repetitive doses of phenylhydrazine (PHZ) and bleeding (BL). A significant uncompensated respiratory alkalosis was found in both groups of anemic rats. BE increased slightly, whereas strong ion difference ([SID]) and weak acid buffers ([A(TOT)]) remained unchanged in anemic rats. The reasons for the absence of compensation for hypocapnia and the differences in the behaviour of acid-base variables are discussed. BE increase was considered paradoxical; its calculation was affected by the experimental conditions and BE had little physiological relevance during anemia. The absence of metabolic renal compensation in anemic rats could be due to a lower pH in the kidney due to anemic hypoxia. Finally, the changes in buffer strength related to low Hb and low P(CO2) might influence plasma [SID] through counteracted shifts of strong ions between erythrocytes and plasma, finally resulting in unchanged [SID] during anemia.
authors.affiliation: Departamento de Fisiología, Facultad de Biología, Universidad de Barcelona, Spain. pesquero@bio.ub.es
authors.forename: J
authors.initials: J
authors.lastname: Pesquero
authors.forename: V
authors.initials: V
authors.lastname: Alfaro
authors.forename: L
authors.initials: L
authors.lastname: Palacios
mesh_terms: D000137:Acid-Base Imbalance / Q000097:blood*; D000472:Alkalosis, Respiratory / Q000097:blood*; D000740:Anemia / Q000097:blood* / Q000139:chemically induced; D000818:Animals; D001639:Bicarbonates / Q000097:blood; D002245:Carbon Dioxide / Q000097:blood; D004912:Erythrocytes / Q000378:metabolism*; D006470:Hemorrhage / Q000097:blood*; D006863:Hydrogen-Ion Concentration; D016857:Hypocapnia / Q000097:blood*; D008297:Male; D010659:Phenylhydrazines; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D001639:Bicarbonates; D010659:Phenylhydrazines; C030299:phenylhydrazine; D002245:Carbon Dioxide