title: Isolation of a new insertion element of Yersinia intermedia closely related to remnants of mobile genetic elements present on Yersinia plasmids harboring the Yop virulon.
abstract: A new insertion element present in two alleles, designated IS1635.1 and IS1635.2, was identified on a plasmid of a Yersinia intermedia strain by hybridization with the Yersinia enterocolitica pYV virulence plasmid. IS1635.1 and IS1635.2 are 861 bp long, carry imperfect inverted terminal repeats and possess a single open reading frame encoding a putative transposase of the IS6 family. A truncated IS1635 element is present immediately downstream of element IS1635.2. The capacity of the IS1635 elements to mediate transposition in Yersinia was demonstrated with a R6K-derived suicide vector, where a kanamycin resistance gene had been inserted between IS1635.1 and IS1635.2. Hybridization and sequence alignments showed that remnants of IS1635-like insertion elements harboring large deletions and point mutations are present on the Yop virulon harboring plasmids of pathogenic Yersinia strains. In a few cases, the IS1635 element has also been found on plasmids of apathogenic Yersinia strains.
authors.affiliation: Robert Koch-Institut, Projekt Horizontaler Gentransfer, Nordufer 20, D-13353, Berlin, Germany. strauche@rki.de
authors.forename: E
authors.initials: E
authors.lastname: Strauch
authors.forename: B
authors.initials: B
authors.lastname: Hoffmann
authors.forename: G
authors.initials: G
authors.lastname: Heins
authors.forename: B
authors.initials: B
authors.lastname: Appel
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D004251:DNA Transposable Elements; D005798:Genes, Bacterial; D016366:Open Reading Frames; D010957:Plasmids; D017354:Point Mutation; D017384:Sequence Deletion; D020079:Terminal Repeat Sequences; D019895:Transposases / Q000235:genetics; D014774:Virulence; D015007:Yersinia / Q000235:genetics* / Q000472:pathogenicity*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D004251:DNA Transposable Elements; C060772:yopM protein, Yersinia; D019895:Transposases