title: Morphological patterns of death by myocytes in arrhythmogenic right ventricular dysplasia.
abstract: There are two forms of nuclear loss from eukaryotic cells: biochemical DNA degradation in apoptosis and nuclear extrusion from the cell body as seen in mammalian erythroblasts. In biopsies of right ventricular myocardium from 8 patients with arrhythmogenic right ventricular dysplasia (ARVD), we found not only a terminal deoxynucleotidyl transferase-mediated digoxigenin-deoxyuridine triphosphate nick-end labeling (TUNEL)-positive nucleus in mononuclear myocytes, but also 1 or 2 TUNEL-positive nuclei in multinuclear myocytes. With electron microscopy, we found a nuclear dislocation to the cell periphery, followed by its extrusion into the extracellular space. Both the migration and extrusion of the nuclei of myocytes resemble the morphogenesis of human erythroblasts. Nuclear extrusion from myocytes may be another form of programmed cell death. In support of this possibility, we also found evidence of cytoplasmic degradation in right ventricular myocytes from our ARVD cases, a process similar to one often seen in developmental programmed cell death and differing from typical nuclear apoptosis. In our ARVD cases, we thus found several different patterns of cell death, all associated with initial preservation of the plasmalemma and avoidance of local inflammation. All these features may be different responses to common signals for selective non-necrotic (apoptotic) death of right ventricular myocytes.
authors.affiliation: Department of Medicine, University of Texas Medical Branch, Galveston 77555-0175, USA.
authors.forename: M S
authors.initials: MS
authors.lastname: Runge
authors.forename: G A
authors.initials: GA
authors.lastname: Stouffer
authors.forename: R G
authors.initials: RG
authors.lastname: Sheahan
authors.forename: S
authors.initials: S
authors.lastname: Yamamoto
authors.forename: V G
authors.initials: VG
authors.lastname: Tsyplenkova
authors.forename: T N
authors.initials: TN
authors.lastname: James
mesh_terms: D000328:Adult; D017209:Apoptosis; D019571:Arrhythmogenic Right Ventricular Dysplasia / Q000473:pathology*; D002467:Cell Nucleus / Q000473:pathology / Q000648:ultrastructure; D003593:Cytoplasm / Q000473:pathology / Q000648:ultrastructure; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D020287:In Situ Nick-End Labeling; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D009206:Myocardium / Q000473:pathology* / Q000648:ultrastructure; D010587:Phagocytosis