InIB-dependent internalization of Listeria is mediated by the Met receptor tyrosine kinase.
The Listeria monocytogenes surface protein InlB promotes bacterial entry into mammalian cells. Here, we identify a cellular surface receptor required for InlB-mediated entry. Treatment of mammalian cells with InlB protein or infection with L. monocytogenes induces rapid tyrosine phosphorylation of Met, a receptor tyrosine kinase (RTK) for which the only known ligand is Hepatocyte Growth Factor (HGF). Like HGF, InlB binds to the extracellular domain of Met and induces "scattering" of epithelial cells. Experiments with Met-positive and Met-deficient cell lines demonstrate that Met is required for InlB-dependent entry of L. monocytogenes. InlB is a novel Met agonist that induces bacterial entry through exploitation of a host RTK pathway.
Shen
Y
Y
Department of Medical Genetics and Microbiology, University of Toronto, Ontario, Canada.
Naujokas
M
M
Park
M
M
Ireton
K
K
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D030161:Avian Proteins; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D002449:Cell Aggregation / Q000187:drug effects; D002460:Cell Line; D003598:Cytoskeletal Proteins / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004705:Endocytosis; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000382:microbiology; D017353:Gene Deletion; D022781:Hepatocytes; D006801:Humans; D007668:Kidney; D007700:Kinetics; D008024:Ligands; D008089:Listeria monocytogenes / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D019000:Phosphotyrosine / Q000378:metabolism; D011233:Precipitin Tests; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D050721:Proto-Oncogene Proteins c-cbl; D019859:Proto-Oncogene Proteins c-met / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D044767:Ubiquitin-Protein Ligases
D048868:Adaptor Proteins, Signal Transducing; D030161:Avian Proteins; D001426:Bacterial Proteins; D003598:Cytoskeletal Proteins; C098587:GAB1 protein, human; D008024:Ligands; D008565:Membrane Proteins; D010750:Phosphoproteins; D011518:Proto-Oncogene Proteins; C095768:inlB protein, Listeria monocytogenes; C069761:internalin protein, Bacteria; C071033:cytoskeletal p85 protein, chicken; D019000:Phosphotyrosine; D050721:Proto-Oncogene Proteins c-cbl; D044767:Ubiquitin-Protein Ligases; D019859:Proto-Oncogene Proteins c-met; C067419:CBL protein, human