[New approaches to construction of transgenic animals with high level tissue-specific expression of foreign genes: construction and reconstruction of genomic domains].
The major problems in constructing transgenic animals and approaches to their solution are discussed. Modern views of the structural-functional organization of the eukaryotic genome are considered. The domain hypothesis of the genome organization is analyzed in detail, as many problems associated with a low level and a lack of tissue specificity of transgene expression can be solved at the step of construction (or reconstruction of existing domains).
Razin
S V
SV
Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia. razin@ijm.jussieu.fr
Verbovaia
L V
LV
Gol'dman
I L
IL
D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D004742:Enhancer Elements, Genetic; D005057:Eukaryotic Cells / Q000378:metabolism; D015870:Gene Expression; D018014:Gene Transfer Techniques; D016678:Genome; D006801:Humans; D007438:Introns; D019901:Locus Control Region; D011401:Promoter Regions, Genetic; D019076:Transgenes