title: Detection of radiation-induced hydrocarbons and 2-alkylcyclobutanones in irradiated perilla seeds.
abstract: The method consists of the extraction of fat from perilla seeds, separation of hydrocarbons and 2-alkylcyclobutanones with florisil column chromatography, and identification of hydrocarbons and 2-alkylcyclobutanones by gas chromatography-mass spectroscopy (GC-MS). Concentrations of hydrocarbons and 2-alkylcyclobutanones increased with the irradiation dose. The major hydrocarbons in the irradiated perilla seeds were 8-heptadecene and 1,7-hexadecadiene from oleic acid and 6,9-heptadecadiene and 1,7,10-hexadecatriene from linoleic acid. One of the 2-alkylcyclobutanones, 2-(5'-tetradecenyl)cyclobutanone, was found in the highest concentration in the irradiated perilla seeds. Radiation-induced hydrocarbons in the perilla seeds were detected at doses of 0.5 kGy and higher, and radiation-induced 2-alkylcyclobutanones were detected at doses of 1 kGy and higher. These compounds were not detected in nonirradiated perilla seeds.
authors.affiliation: Department of Food and Nutrition, Chosun University, Kwangju, Korea.
authors.forename: H J
authors.initials: HJ
authors.lastname: Lee
authors.forename: M W
authors.initials: MW
authors.lastname: Byun
authors.forename: K S
authors.initials: KS
authors.lastname: Kim
mesh_terms: D002074:Butanones / Q000032:analysis* / Q000737:chemistry; D002845:Chromatography / Q000379:methods; D005227:Fatty Acids / Q000378:metabolism / Q000528:radiation effects; D005514:Food Irradiation; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006838:Hydrocarbons / Q000032:analysis*; D019686:Lamiaceae / Q000737:chemistry / Q000528:radiation effects; D011829:Radiation Dosage; D012639:Seeds / Q000737:chemistry / Q000528:radiation effects
chemical_list: D002074:Butanones; D005227:Fatty Acids; D006838:Hydrocarbons