Lymphocyte mitogenic transformation is accompanied by phosphorylation of the nucleolar transcription factor UBF.
Lymphoid cell stimulation with phytohemagglutinin, converting quiescent lymphocytes into rapidly-proliferating lymphoblast-like cells, is tightly coordinated with the transcription of ribosomal genes. Nuclear Run-On assays demonstrated that after the addition of PHA ribosomal gene activity increased rapidly during the first 40 hrs. and within 64 hrs. it reached its maximum. In contrast, UBF1 and UBF2 intracellular levels, examined in immunoblots, paralleled DNA replication/cell proliferation activity. During the first 40 hrs. following the addition of PHA, lymphocytes did not proliferate and UBFI and UBF2 levels nearly corresponded to that found in quiescent cells. However, the UBF1 and UBF2 intracellular contents increased nearly twofold in the interval from 40 to 64 hrs. Metabolic radiolabeling with 32P-orthophosphate showed that in quiescent lymphocytes the cellular pool of UBF proteins was in an underphosphorylated state. Within the first 40 hrs. after the addition of PHA the phosphorylation of underphosphorylated UBF1 and UBF2 pools predominated over the phosphorylation of very small amounts of neosynthesized UBF molecules. Subsequently, within the time period from 48 to 64 hrs. the phosphorylation of neosynthesized UBF proteins predominated. We speculate that the phosphorylation-mediated mobilization of the inactive underphosphorylated intracellular UBF pool during the G1 phase of the first cell division cycle is implicated in the ribosomal RNA synthesis acceleration and in the transformation of quiescent lymphocytes to lymphoblast-like proliferating cells.
Kalousek
I
I
Department of Cellular Biochemistry, Institute of Hematology and Blood Transfusion, Prague, Czech Republic. kal@uhkt.cz
Krízková
P
P
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000378:metabolism*; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology; D008934:Mitogens / Q000276:immunology / Q000494:pharmacology*; D010766:Phosphorylation / Q000187:drug effects; D010835:Phytohemagglutinins / Q000276:immunology / Q000494:pharmacology; D035463:Pol1 Transcription Initiation Complex Proteins; D012322:RNA Precursors / Q000096:biosynthesis; D012335:RNA, Ribosomal / Q000096:biosynthesis; D014157:Transcription Factors / Q000096:biosynthesis / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects
D004268:DNA-Binding Proteins; D008934:Mitogens; D010835:Phytohemagglutinins; D035463:Pol1 Transcription Initiation Complex Proteins; D012322:RNA Precursors; D012335:RNA, Ribosomal; D014157:Transcription Factors; C063844:transcription factor UBF; D004247:DNA