Differences in Brazilian strains of Schistosoma mansoni evaluated by means of morphometric analysis of cercariae of both sexes.
Morphometrics of Brazilian strains (BH, SJ and CMO) of Schistosoma mansoni cercariae were obtained with a computerized image analyzer (IMAGE PRO PLUS, MEDIA CYBERNETICS), considering the following characters: body area, tail, furcae, oral and ventral suckers and distance between them. For statistical analysis, the variance test (one-way Anova) was applied and significant differences of p< 0.05 were considered. All morphometric values in the BH strain were significantly higher (p< 0.05) than in the others. Lower values were obtained in females of SJ strain for all characters, excepting the body area. Only this character showed to be significantly different in males and females of the three strains. Specimens of both sexes in the BH and SJ strains showed significant differences regarding all characters. It was observed that this morphometric analysis permits the characterization of strains and also the sex identification in S. mansoni cercariae. Due to its feasibility, this method can be applied as a tool in laboratories devoid of more complex equipment.
Machado-Silva
J R
JR
Departamento de Patologia e Laboratórios, Faculdade de Ciências Médicas, Uerj, Rio de Janeiro, RJ, Brasil.
da Silva
C H
CH
Pereira
M J
MJ
de Oliveira
R M
RM
Pinto
R M
RM
Gomes
D C
DC
D000704:Analysis of Variance; D000818:Animals; D001938:Brazil; D005260:Female; D008297:Male; D051379:Mice; D012550:Schistosoma mansoni / Q000145:classification* / Q000502:physiology; D012727:Sex Characteristics; D013045:Species Specificity