L-Selenohomocysteine: one-step synthesis from L-selenomethionine and kinetic analysis as substrate for methionine synthases.
A single-step convenient synthesis of L-selenohomocysteine (SeHcy) from L-selenomethionine (SeMet) using sodium in liquid ammonia is described. Methionine synthases convert SeHcy to SeMet at rates comparable to their rates of conversion of L-homocysteine (Hcy) to L-methionine (Met). This study suggests that SeHcy generated from SeMet metabolism can be efficiently recycled to SeMet in mammals.
Zhou
Z S
ZS
Department of Biological Chemistry, The University of Michigan, Ann Arbor 48109-1055, USA.
Smith
A E
AE
Matthews
R G
RG
D013766:5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase / Q000378:metabolism*; D000818:Animals; D016588:Anticarcinogenic Agents / Q000138:chemical synthesis / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000378:metabolism*; D005260:Female; D006801:Humans; D007700:Kinetics; D008297:Male; D015394:Molecular Structure; D016566:Organoselenium Compounds / Q000138:chemical synthesis* / Q000378:metabolism*; D010084:Oxidation-Reduction; D012643:Selenium / Q000378:metabolism*; D017279:Selenocysteine / Q000031:analogs & derivatives; D012645:Selenomethionine / Q000737:chemistry / Q000378:metabolism*
D016588:Anticarcinogenic Agents; D016566:Organoselenium Compounds; D017279:Selenocysteine; D012645:Selenomethionine; D013766:5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase; D012643:Selenium; D003545:Cysteine; C002979:selenomethylselenocysteine