title: Pax6 activity in the lens primordium is required for lens formation and for correct placement of a single retina in the eye.
abstract: The Pax6 transcription factor plays a key role in ocular development of vertebrates and invertebrates. Homozygosity of the Pax6 null mutation in human and mice results in arrest of optic vesicle development and failure to initiate lens formation. This phenotype obscures the understanding of autonomous function of Pax6 in these tissue components and during later developmental stages. We employed the Cre/loxP approach to inactivate Pax6 specifically in the eye surface ectoderm concomitantly with lens induction. Although lens induction occurred in the mutant, as indicated by Sox2 up-regulation in the surface ectoderm, further development of the lens was arrested. Hence, Pax6 activity was found to be essential in the specified ectoderm for lens placode formation. Furthermore, this mutant model allowed us for the first time to address in vivo the development of a completely normal retina in the absence of early lens structures. Remarkably, several independent, fully differentiated neuroretinas developed in a single optic vesicle in the absence of a lens, demonstrating that the developing lens is not necessary to instruct the differentiation of the neuroretina but is, rather, required for the correct placement of a single retina in the eye.
authors.affiliation: Max-Planck Institute of Biophysical Chemistry, Department of Molecular Cell Biology, Goettingen D-37077, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Ashery-Padan
authors.forename: T
authors.initials: T
authors.lastname: Marquardt
authors.forename: X
authors.initials: X
authors.lastname: Zhou
authors.forename: P
authors.initials: P
authors.lastname: Gruss
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D004268:DNA-Binding Proteins / Q000096:biosynthesis; D004475:Ectoderm / Q000502:physiology; D005124:Eye Abnormalities / Q000235:genetics*; D005136:Eye Proteins / Q000096:biosynthesis; D005246:Feedback; D017353:Gene Deletion; D018507:Gene Expression Regulation, Developmental; D024002:HMGB Proteins; D018398:Homeodomain Proteins / Q000235:genetics / Q000502:physiology*; D006801:Humans; D007908:Lens, Crystalline / Q000002:abnormalities / Q000196:embryology*; D051379:Mice; D018345:Mice, Knockout; D008817:Mice, Mutant Strains; D009024:Morphogenesis / Q000235:genetics; D009687:Nuclear Proteins / Q000096:biosynthesis; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; D012097:Repressor Proteins; D012160:Retina / Q000002:abnormalities / Q000196:embryology*; D055748:SOXB1 Transcription Factors; D014157:Transcription Factors
chemical_list: D004268:DNA-Binding Proteins; D005136:Eye Proteins; D024002:HMGB Proteins; D018398:Homeodomain Proteins; D009687:Nuclear Proteins; D000071841:PAX6 Transcription Factor; C000605730:PAX6 protein, human; D051761:Paired Box Transcription Factors; C000605732:Pax6 protein, mouse; D012097:Repressor Proteins; C527782:SOX2 protein, human; D055748:SOXB1 Transcription Factors; C527783:Sox2 protein, mouse; D014157:Transcription Factors