The capability of isolated hepatocytes and liver slices of donor livers to predict graft function after liver transplantation.
OBJECTIVE
In liver transplantation, adequate function tests for donor livers and transplanted livers are of utmost importance to provide an objective basis for decision-making. Isolated hepatocyte and/or slice preparations from human donor liver tissue may be suitable to test the quality of the organ to be transplanted.


METHODS
Surgical waste material remaining after reduced size or split liver transplantation in children was used to prepare slices and isolated hepatocytes. The viability of these preparations as well as drug transport and metabolism functions were determined and related to graft function in 32 liver recipients.


RESULTS
The in vitro tests used in the present study apparently did not select non-viable livers. In vitro preparations of the primary non-function grafts which occurred in the investigated group showed normal viability, metabolic and uptake function.


CONCLUSIONS
These results indicate that either the presently used viability tests are not sensitive enough to detect potential organ failure or that other factors besides the hepatocyte viability at the time of transplantation are of paramount importance to the graft function of the recipient, such as complications during and after transplantation or the viability of the non-parenchymal cells.
Olinga
P
P
Department of Pharmacokinetics and Drug Delivery, University Centre for Pharmacy, Groningen University Institute for Drug Exploration, The Netherlands.
Maring
J K
JK
Merema
M
M
Hof
I H
IH
Slooff
M J
MJ
Meijer
D K
DK
Groothuis
G M
GM
D000255:Adenosine Triphosphate / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D001692:Biological Transport; D002452:Cell Count; D002470:Cell Survival; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D046508:Culture Techniques; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008012:Lidocaine / Q000378:metabolism; D008099:Liver / Q000166:cytology* / Q000378:metabolism* / Q000601:surgery; D008111:Liver Function Tests; D016031:Liver Transplantation; D008297:Male; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D013656:Taurocholic Acid / Q000378:metabolism
D013656:Taurocholic Acid; D000255:Adenosine Triphosphate; D008012:Lidocaine