Dimethyl suberimidate cross-linking of oligo(dT) to DNA-binding proteins.
Dimethyl suberimidate is a bifunctional reagent that is used for cross-linking the protein components of oligomeric macromolecules. In this report, dimethyl suberimidate is shown to specifically cross-link oligo(dT) of varying lengths to the DNA-binding subunits of a multimeric helicase-primase encoded by herpes simplex virus type 1. This result indicates that dimethyl suberimidate and other imidoester cross-linking reagents may be useful for characterizing the interaction of oligo(dT) with proteins that bind single-stranded DNA.
Dodson
M S
MS
Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, Arizona 85721-0088, USA.
D003432:Cross-Linking Reagents / Q000737:chemistry*; D004265:DNA Helicases / Q000737:chemistry; D019915:DNA Primase; D004268:DNA-Binding Proteins / Q000737:chemistry*; D004120:Dimethyl Suberimidate / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D014764:Viral Proteins / Q000737:chemistry
D003432:Cross-Linking Reagents; D004268:DNA-Binding Proteins; D009838:Oligodeoxyribonucleotides; D014764:Viral Proteins; C027903:oligo (dT); D004120:Dimethyl Suberimidate; D019915:DNA Primase; C062021:helicase-primase, Human herpesvirus 1; D004265:DNA Helicases