Nuclear transfer in cattle : effect of linoleic acid-albumin on freezing sensitivity of enucleated oocytes.
The effect of linoleic acid-albumin (LAA) supplementation to the media for IVM, enucleation, and activation on the developmental potential of bovine embryos produced by nuclear transfer (NT) into frozen-thawed cytoplasts was investigated. Blastomeres derived from morulae was placed in the perivitelline space of frozen-thawed cytoplasts, which were then fused by a DC pulse. The proportion of fused embryos was similar between groups with and without LAA (87 vs. 90%). The proportion of development to blastocysts of NT embryos derived from the media with LAA (14%) was higher than that without LAA (4%), indicating that LAA treatment of bovine oocytes during IVM, enucleation and activation can improve the ability of such cytoplasts after freezing and thawing to develop into blastocysts after NT.
Hochi
S
S
Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano, Japan.
Kato
M
M
Ito
K
K
Hirabayashi
M
M
Ueda
M
M
Sekimoto
A
A
Nagao
Y
Y
Kimura
K
K
Hanada
A
A
D000418:Albumins; D000818:Animals; D002417:Cattle / Q000502:physiology*; D015925:Cryopreservation / Q000662:veterinary; D003470:Culture Media; D005615:Freezing; D018014:Gene Transfer Techniques / Q000662:veterinary*; D019787:Linoleic Acid; D009865:Oocytes / Q000502:physiology*
D000418:Albumins; D003470:Culture Media; D019787:Linoleic Acid