Differential inhibition of human platelet aggregation by selected Allium thiosulfinates.
Thiosulfinates (TSs) have been implicated as a principle source of the antiplatelet property of raw onion and garlic juice. The in vitro responses of human platelets to dosages of four TSs were measured using whole blood aggregometry and compared by regression analysis. Of the compounds evaluated, methyl methane-TS (MMTS), propyl propane-TS (PPTS), and 2-propenyl 2-propene-TS (allicin) are present in freshly cut Allium vegetables, whereas ethyl ethane-TS (EETS) has not been detected. All TSs were synthesized using a model reaction system. PPTS and allicin had the strongest antiplatelet activity at 0.4 mM, inhibiting aggregation by 90 and 89%, respectively. At the same concentration, EETS and MMTS were significantly weaker, inhibiting 74 and 26%, respectively. Combinations of TSs were not additive in their inhibition of aggregation, indicating that the antiplatelet potential of Allium extracts cannot be easily predicted by quantifying organosulfur components. EETS, PPTS, and allicin were significantly more potent platelet inhibitors than aspirin at nearly equivalent concentrations.
Briggs
W H
WH
Departments of Horticulture and Food Science, University of Wisconsin-Madison, 1575 Linden Drive, Madison, Wisconsin 53706, USA.
Xiao
H
H
Parkin
K L
KL
Shen
C
C
Goldman
I L
IL
D000490:Allium; D004220:Disulfides; D006801:Humans; D008741:Methyl Methanesulfonate / Q000031:analogs & derivatives* / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D012044:Regression Analysis; D013329:Structure-Activity Relationship; D013441:Sulfinic Acids / Q000494:pharmacology*; D013451:Sulfonic Acids / Q000494:pharmacology*
D004220:Disulfides; D010975:Platelet Aggregation Inhibitors; D013441:Sulfinic Acids; D013451:Sulfonic Acids; C014674:methyl methanethiosulfonate; C006452:allicin; D008741:Methyl Methanesulfonate