Higher macrophage inflammatory protein (MIP)-1alpha and MIP-1beta levels from CD8+ T cells are associated with asymptomatic HIV-1 infection.
To test the hypothesis that beta-chemokine levels may be relevant to the control of HIV in vivo, we compared RANTES, MIP-1alpha, and MIP-1beta production from purified CD8(+) T cells from 81 HIV-infected subjects and from 28 uninfected donors. Asymptomatic HIV(+) subjects produced significantly higher levels of MIP-1alpha and MIP-1beta, but not RANTES, than uninfected donors or patients that progressed to AIDS. In contrast, beta chemokines in plasma were either nondetectable or showed no correlation with clinical status. The high beta-chemokine-mediated anti-HIV activity was against the macrophage tropic isolate HIV-1(BAL), with no demonstrable effect on the replication of the T-cell tropic HIV-1(IIIB). These findings suggest that constitutive beta-chemokine production may play an important role in the outcome of HIV-1 infection.
Cocchi
F
F
Institute of Human Virology, University of Maryland Biotechnology Institute, University of Maryland, 725 West Lombard Street, Baltimore, MD 21201-1192, USA. cocchi@umbi.umd.edu
DeVico
A L
AL
Yarchoan
R
R
Redfield
R
R
Cleghorn
F
F
Blattner
W A
WA
Garzino-Demo
A
A
Colombini-Hatch
S
S
Margolis
D
D
Gallo
R C
RC
D000998:Antiviral Agents / Q000502:physiology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D054377:Chemokine CXCL12; D019743:Chemokines, CXC / Q000502:physiology; D015658:HIV Infections / Q000097:blood*; D015497:HIV-1 / Q000302:isolation & purification; D006801:Humans; D019402:Macrophage Inflammatory Proteins / Q000096:biosynthesis / Q000097:blood*
D000998:Antiviral Agents; C515255:CXCL12 protein, human; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D054377:Chemokine CXCL12; D019743:Chemokines, CXC; D019402:Macrophage Inflammatory Proteins