title: Disruption of klotho gene causes an abnormal energy homeostasis in mice.
abstract: klotho mice, which genetically lack klotho gene expression, are characterized with various systemic phenotypes resembling human aging, and also with growth retardation. Here we show that klotho mice have a barely detectable amount of the white adipose tissue but their brown adipose tissue (BAT) is comparably preserved. Glucose tolerance and insulin sensitivity in klotho mice are increased compared to those in wild-type mice as revealed by intraperitoneal glucose and insulin tolerance tests. Uncoupling protein-1 gene expression of BAT and body temperature in klotho mice are lower than those in wild-type mice, suggesting that klotho mice have less energy expenditure than wild-type mice. Histological examination suggests that klotho mice possess less energy storage than wild-type mice with respect to glycogen in the liver and lipid in BAT. All these changes of parameters for energy homeostasis in klotho mice are very similar to those reported under food-restricted conditions. However, the amount of food intake is not different between klotho and wild-type mice when normalized for body weight. The present study elucidates the importance of klotho gene expression for the maintenance of normal energy homeostasis.
authors.affiliation: Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Kyoto, 606-8507, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Mori
authors.forename: K
authors.initials: K
authors.lastname: Yahata
authors.forename: M
authors.initials: M
authors.lastname: Mukoyama
authors.forename: T
authors.initials: T
authors.lastname: Suganami
authors.forename: H
authors.initials: H
authors.lastname: Makino
authors.forename: T
authors.initials: T
authors.lastname: Nagae
authors.forename: H
authors.initials: H
authors.lastname: Masuzaki
authors.forename: Y
authors.initials: Y
authors.lastname: Ogawa
authors.forename: A
authors.initials: A
authors.lastname: Sugawara
authors.forename: Y
authors.initials: Y
authors.lastname: Nabeshima
authors.forename: K
authors.initials: K
authors.lastname: Nakao
mesh_terms: D000273:Adipose Tissue / Q000033:anatomy & histology*; D002001:Adipose Tissue, Brown / Q000033:anatomy & histology* / Q000473:pathology; D000375:Aging; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight; D004734:Energy Metabolism / Q000235:genetics*; D005966:Glucuronidase; D006706:Homeostasis; D006801:Humans; D007328:Insulin / Q000494:pharmacology / Q000502:physiology; D000090265:Klotho Proteins; D008099:Liver / Q000201:enzymology / Q000473:pathology; D008112:Liver Glycogen / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008817:Mice, Mutant Strains; D023421:Models, Animal; D009929:Organ Size; D010179:Pancreas / Q000473:pathology; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000235:genetics
chemical_list: D001786:Blood Glucose; D007328:Insulin; D008112:Liver Glycogen; D008565:Membrane Proteins; D005966:Glucuronidase; D000090265:Klotho Proteins; D010729:Phosphoenolpyruvate Carboxykinase (GTP)