Mapping of five new putative anion transporter genes in human and characterization of SLC26A6, a candidate gene for pancreatic anion exchanger.
A second distinct family of anion transporters, in addition to the classical SLC4 (or AE) family, has recently been delineated. Members of the SLC26 family are structurally well conserved and can mediate the electroneutral exchange of Cl(-) for HCO(-)(3) across the plasma membrane of mammalian cells like members of the SLC4 family. Three human transporter proteins have been functionally characterized: SLC26A2 (DTDST), SLC26A3 (CLD or DRA), and SLC26A4 (PDS) can transport with different specificities the chloride, iodine, bicarbonate, oxalate, and hydroxyl anions, whereas SLC26A5 (prestin) was suggested to act as the motor protein of the cochlear outer hair cell. We report the expansion of the SLC26 family with five new members in chromosomes 3, 6, 8, 12, and 17 and mapping of SLC26A1 to 4p16.3. We have characterized one of them, SLC26A6, in more detail. It maps to chromosome 3p21.3, encodes a predicted 738-amino-acid transmembrane protein, and is most abundantly expressed in the kidney and pancreas. Pancreatic ductal cell lines Capan-1 and Capan-2 express SLC26A6, and immunohistochemistry localizes SLC26A6 protein to the apical surface of pancreatic ductal cells, suggesting it as a candidate for a luminal anion exchanger. The functional characterization of the novel members of this tissue-specific gene family may provide new insights into anion transport physiology in different parts of the body.
Lohi
H
H
Department of Medical Genetics, University of Helsinki, Helsinki, Finland.
Kujala
M
M
Kerkelä
E
E
Saarialho-Kere
U
U
Kestilä
M
M
Kere
J
J
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D027321:Anion Transport Proteins; D000838:Anions / Q000378:metabolism*; D001483:Base Sequence; D001692:Biological Transport; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007684:Kidney Tubules; D008958:Models, Molecular; D008969:Molecular Sequence Data; D005810:Multigene Family; D010179:Pancreas / Q000378:metabolism*; D011110:Polymorphism, Genetic; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D027321:Anion Transport Proteins; D000838:Anions; D002352:Carrier Proteins