Inhibition of chemotaxis by organic acids from anaerobes may prevent a purulent response in bacterial vaginosis.
It has been postulated that certain organic acids produced by the anaerobes associated with bacterial vaginosis (BV) could prevent a purulent response in this infection. Varying concentrations of pure succinic, acetic and lactic acids were incubated in vitro with a monocytic cell line (MonoMac 6). High inhibition of chemotaxis was produced by succinic acid; lower inhibition and no inhibition was shown by acetic acid and lactic acid respectively. Succinic and acetic acids were detected in high concentrations in the vaginal fluid of women with BV and in culture supernates of Prevotella and Mobiluncus spp.; these acids impaired chemotaxis of MonoMac 6 cells in vitro. The vaginal fluids of normal women and the culture supernates of Lactobacillus spp. had no effect on chemotaxis.
Al-Mushrif
S
S
Division of Molecular and Genetic Medicine, University of Sheffield Medical School, Sheffield, S10 2RX.
Eley
A
A
Division of Molecular and Genetic Medicine, University of Sheffield Medical School, Sheffield, S10 2RX.
Jones
B M
BM
Division of Molecular and Genetic Medicine, University of Sheffield Medical School, Sheffield, S10 2RX.
D001421:Bacteria, Anaerobic / Q000737:chemistry*; D002460:Cell Line; D002634:Chemotaxis, Leukocyte / Q000187:drug effects / Q000276:immunology*; D002849:Chromatography, Gas; D005227:Fatty Acids / Q000096:biosynthesis / Q000494:pharmacology / Q000502:physiology*; D005260:Female; D006194:Gardnerella vaginalis / Q000378:metabolism; D006801:Humans; D007778:Lactobacillus / Q000378:metabolism; D017904:Mobiluncus / Q000378:metabolism; D009000:Monocytes / Q000187:drug effects / Q000276:immunology*; D018720:Prevotella / Q000378:metabolism; D014621:Vagina / Q000737:chemistry / Q000382:microbiology; D016585:Vaginosis, Bacterial / Q000382:microbiology / Q000517:prevention & control*
D005227:Fatty Acids