Isolation and sequence analysis of the trpBA gene cluster, encoding tryptophan synthase, from Azospirillum brasilense.
The trpBA gene cluster of Azospirillum brasilense Sp7 was isolated by complementation of an Escherichia coli trpBA mutant. Both genes code for the two subunits of tryptophan synthase, which catalyzes the last step in tryptophan biosynthesis. No structural features indicating transcriptional regulation could be identified. Upstream of the trpBA cluster an open reading frame encoding a putative periplasmic binding protein, involved in amino acid transport, was identified. Analysis of the downstream region of the trpBA cluster revealed the presence of a putative open reading frame encoding a subunit of the acetyl-coenzyme A carboxylase carboxyl transferase complex.
Dosselaere
F
F
F.A. Janssens Laboratory of Genetics, K. U. Leuven, Heverlee, Belgium.
Lambrecht
M
M
Vanderleyden
J
J
D000595:Amino Acid Sequence; D015806:Azospirillum brasilense / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames; D021122:Protein Subunits; D015183:Restriction Mapping; D016961:Rhizobium leguminosarum / Q000201:enzymology / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D014367:Tryptophan Synthase / Q000737:chemistry / Q000235:genetics*
D021122:Protein Subunits; D014367:Tryptophan Synthase