p(1),p(4)-diadenosine 5'-tetraphosphate induces the uptake of arginine and citrulline by a pore on the plasma membrane of bovine aortic endothelial cells.
We have previously demonstrated that p(1),p(4)-diadenosine 5'-tetraphosphate (Ap(4)A) induces the release of nitric oxide (NO) and modulates the uptake of extracellular L-arginine (L-Arg) and L-citrulline (L-Cit) by bovine aortic endothelial cells (BAEC) [Hilderman, R.H. and Christensen, E.F. (1998) FEBS Lett. 427, 320-324 and Hilderman, R.H., Casey, T.E. and Pojoga, L.H. (2000) Arch. Biochem. Biophys. 375, 124-130]. In this communication we report that extracellular Ap(4)A enhances the uptake of L-Arg and L-Cit through a pore on the plasma membrane of BAEC that is selective for these two amino acids. We also demonstrate that Ap(2)A, which induces NO release, enhances L-Arg uptake while Ap(5)A, a vasoconstrictor, does not enhance the uptake of L-Arg. The potential physiological significance of the uptake of these two amino acids in relation to NO synthesis is discussed.
Cooper
H M
HM
Department of Biomedical Sciences, Clemson University, SC 29634-1903, USA.
Rosenberg
C R
CR
Hilderman
R H
RH
D000818:Animals; D001011:Aorta; D001120:Arginine / Q000378:metabolism*; D001125:Argininosuccinic Acid / Q000378:metabolism; D001345:Autoradiography; D002417:Cattle; D021961:Cell Membrane Structures / Q000187:drug effects* / Q000378:metabolism* / Q000648:ultrastructure; D002478:Cells, Cultured; D002956:Citrulline / Q000378:metabolism*; D003513:Cycloheximide / Q000494:pharmacology; D015226:Dinucleoside Phosphates / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D013498:Suramin / Q000494:pharmacology; D013696:Temperature; D014316:Tritium
D015226:Dinucleoside Phosphates; D011500:Protein Synthesis Inhibitors; D014316:Tritium; D001125:Argininosuccinic Acid; D002956:Citrulline; D009569:Nitric Oxide; C020733:diadenosine tetraphosphate; D013498:Suramin; D001120:Arginine; D003513:Cycloheximide; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III