title: The rpoN gene of Enterococcus faecalis directs sensitivity to subclass IIa bacteriocins.
abstract: The final sigma 54 factor has been previously described to be involved in Listeria monocytogenes sensitivity to mesentericin Y105, a subclass IIa bacteriocin. Here, we identified the rpoN gene, encoding final sigma 54, of Enterococcus faecalis JH2-2 and showed that its interruption leads to E. faecalis resistance to different subclass IIa bacteriocins. Moreover, this rpoN mutant remained sensitive to nisin, a class I bacteriocin, suggesting that final sigma 54 is especially involved in sensitivity to subclass IIa bacteriocins.
authors.affiliation: Laboratoire de Biologie Moléculaire, CNRS ESA 6031, IBMIG, Université de Poitiers, 40 avenue du recteur Pineau, 86022 Poitiers Cedex, France.
authors.forename: K
authors.initials: K
authors.lastname: Dalet
authors.forename: C
authors.initials: C
authors.lastname: Briand
authors.forename: Y
authors.initials: Y
authors.lastname: Cenatiempo
authors.forename: Y
authors.initials: Y
authors.lastname: Héchard
mesh_terms: D001430:Bacteriocins / Q000145:classification / Q000494:pharmacology*; D004268:DNA-Binding Proteins; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D004352:Drug Resistance, Microbial / Q000235:genetics; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000235:genetics*; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D051877:RNA Polymerase Sigma 54; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D012808:Sigma Factor / Q000235:genetics*
chemical_list: D001430:Bacteriocins; D004268:DNA-Binding Proteins; D012808:Sigma Factor; D012321:DNA-Directed RNA Polymerases; D051877:RNA Polymerase Sigma 54