title: Mitochondrial RNAs of myxomycetes terminate with non-encoded 3' poly(U) tails.
abstract: We examined the 3' ends of edited RNAs from the myxomycetes Stemonitis flavogenita and Physarum polycephalum using a modified anchor PCR approach. Surprisingly, we found that poly(A) tails are missing from the cytochrome c oxidase subunit 1 mRNA (coI) from both species and the cytochrome c oxidase subunit 3 mRNA (cox3) from P. polycephalum. Instead, non-encoded poly(U) tails of varying length were discovered at the 3' ends of these transcripts. These are the first described examples of 3' poly(U) tails on mature mRNAs in any system.
authors.affiliation: Departments of Ecology and Evolutionary Biology, Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
authors.forename: T L
authors.initials: TL
authors.lastname: Horton
authors.forename: L F
authors.initials: LF
authors.lastname: Landweber
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D008928:Mitochondria / Q000235:genetics*; D008969:Molecular Sequence Data; D009235:Myxomycetes / Q000235:genetics*; D016839:Physarum polycephalum / Q000235:genetics; D011072:Poly U / Q000235:genetics*; D021122:Protein Subunits; D012313:RNA / Q000235:genetics*; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D016415:Sequence Alignment; D017421:Sequence Analysis; D012689:Sequence Homology, Nucleic Acid
chemical_list: D020413:3' Untranslated Regions; D018076:DNA, Complementary; D021122:Protein Subunits; D012337:RNA, Ribosomal, 18S; D012338:RNA, Ribosomal, 23S; D011072:Poly U; D012313:RNA; D003576:Electron Transport Complex IV