Alterations of Rb, p16(INK4A) and cyclin D1 in the tumorigenesis of oral squamous cell carcinomas.
Immunohistochemical analysis of Rb, p16(INK4A) and cyclin D1 expression was performed on 78 oral squamous cell carcinoma (SCC), 46 leukoplakia, and 20 normal mucosa. Rb and p16(INK4A) expression were observed in all normal mucosa and most of leukoplakia. Lack of Rb and p16(INK4A) was observed in 56.4 and 67.9% of SCC, respectively. The overexpression of cyclin D1 was not observed in normal mucosa and was observed in 35.9% of SCC. A strong reciprocal relationship between Rb and p16(INK4A) expression was observed in oral SCC, and all these SCC cases have at least one of the alterations in the Rb pathway.
Nakahara
Y
Y
Department of Oral and Maxillofacial Surgery II, Okayama University Dental School, 2-5-1 Shikata, Okayama, 700-8525, Okayama, Japan. nakahara@dent.okayama-u.ac.jp
Shintani
S
S
Mihara
M
M
Kiyota
A
A
Ueyama
Y
Y
Matsumura
T
T
D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D002352:Carrier Proteins / Q000032:analysis*; D019938:Cyclin D1 / Q000032:analysis*; D019941:Cyclin-Dependent Kinase Inhibitor p16; D006801:Humans; D007150:Immunohistochemistry; D009062:Mouth Neoplasms / Q000378:metabolism* / Q000473:pathology; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D016160:Retinoblastoma Protein / Q000032:analysis*
D002352:Carrier Proteins; D019941:Cyclin-Dependent Kinase Inhibitor p16; D018809:Proliferating Cell Nuclear Antigen; D016160:Retinoblastoma Protein; D019938:Cyclin D1