title: Poly(ADP-ribose) polymerase localizes to the centrosomes and chromosomes.
abstract: Poly(ADP-ribose) polymerase (PARP) takes part mainly in regulation of DNA repair, thereby maintaining genomic stability in the nucleus. However, what role PARP plays in mitotic cells is not known. Centrosomes play an important role in maintaining the fidelity of chromosome distribution during cell division. Loss of these functions might cause chromosomal instability and aneuploidy. p53 and BRCA1 were recently found to localize to the centrosome at mitosis. We found that PARP is localized to the centrosomes and the chromosomes at cell-division phase and interphase by indirect immunofluorescence. Furthermore, by analysis of isolated centrosomes PARP protein was found to associate with the centrosomes during mitosis. These data suggest that PARP may be involved in maintenance of chromosomal stability.
authors.affiliation: Department of Biochemistry and Molecular Oncology, Institute of Basic Medical Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki, 305-8575, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kanai
authors.forename: M
authors.initials: M
authors.lastname: Uchida
authors.forename: S
authors.initials: S
authors.lastname: Hanai
authors.forename: N
authors.initials: N
authors.lastname: Uematsu
authors.forename: K
authors.initials: K
authors.lastname: Uchida
authors.forename: M
authors.initials: M
authors.lastname: Miwa
mesh_terms: D002460:Cell Line; D018385:Centrosome / Q000201:enzymology*; D002877:Chromosomes, Human / Q000201:enzymology*; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D008938:Mitosis; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D011065:Poly(ADP-ribose) Polymerases