Anti-transforming nature of ascorbic acid and its derivatives examined by two-stage cell transformation using BALB/c 3T3 cells.
The anti-transforming effects of sodium ascorbate and its stable derivatives were examined in the two-stage transformation assay. When BALB/c 3T3 cells were treated with 0.2 microg/ml 20-methylcholanthrene as an initiator, and 100 ng/ml 12-O-tetradecanoylphorbol-13-acetate as a promoter, the addition at the promotion stage of L-ascorbic acid-2-phosphate ester magnesium (APM) was most marked in the inhibition of transformation. The inhibitory effects of sodium ascorbate and ascorbic acid-2-glucoside (AG) were comparable, but weaker than those of APM; L (+)-ascorbic acid-2-sulfate ester disodium 2H(2)O showed little effect. When phorbol 12, 13-didecanoate or tumor necrosis factor alpha (TNF-alpha) were used as promoters, APM also effectively suppressed transformation.
Tsuchiya
T
T
Safety Evaluation Center, Central Research Laboratory, Showa Denko K. K., Ohnodai 1-1-1, Midori-ku, 267-0056, Chiba, Japan. tsuchiya@ctl.sdk.co.jp
Kato-Masatsuji
E
E
Tsuzuki
T
T
Umeda
M
M
D016475:3T3 Cells; D000818:Animals; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology*; D002471:Cell Transformation, Neoplastic / Q000187:drug effects* / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D008748:Methylcholanthrene / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D014409:Tumor Necrosis Factor-alpha; C001956:ascorbic acid 2-sulfate; C065378:ascorbic acid 2-O-glucoside; D008748:Methylcholanthrene; D013755:Tetradecanoylphorbol Acetate; D001205:Ascorbic Acid