Antigenicity, expression, and molecular characterization of surface-located pullulanase of Streptococcus pneumoniae.
A putative pullulanase-encoding gene from Streptococcus pneumoniae was identified by screening a genomic expression library with human convalescent-phase serum. The 3,864-bp gene encoded a 143-kDa protein. Surface location and pullulanase activity of the protein, designated SpuA, was demonstrated. SpuA was present in all investigated pneumococcal isolates of different serotypes. The spuA 5' end was highly conserved among clinical isolates except for a 75-bp region. The properties of SpuA reported here indicate that this novel immunogenic surface protein might have potential as a vaccine target.
Bongaerts
R J
RJ
Department of Medical Microbiology and Virology, University of Duesseldorf, Duesseldorf, Germany.
Heinz
H P
HP
Hadding
U
U
Zysk
G
G
D000595:Amino Acid Sequence; D001483:Base Sequence; D017124:Conserved Sequence; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data; D013296:Streptococcus pneumoniae / Q000201:enzymology* / Q000276:immunology / Q000472:pathogenicity; D014774:Virulence
D006026:Glycoside Hydrolases; C100181:pullulanase