Cloning and characterization of a Candida albicans gene homologous to fructose-1,6-bisphosphatase genes.
By sequencing of the DNA adjacent to the Candida albicans SEC61 gene, an open reading frame encoding a polypeptide of 331 amino acids was found. The predicted protein showed a strong homology with the fructose-1,6-bisphosphatase [FbPase] from other organisms, and conserved regions included the catalytic motif found in all known FbPases. Although the cloned gene did not complement the growth failure of a Saccharomyces cerevisiae fbp1 mutant in media with gluconeogenic carbon sources, it was transcribed in the transformants in a fashion that indicates a partial repression by glucose. A similar control on the transcription of this gene and on FbPase activity was found in wild-type C. albicans, where the cloned gene (CaFBP1) was shown to be localized in a single chromosomal locus in the genome.
De la Rosa
J M
JM
Departamento de Microbiología y Biología Celular, Facultad de Farmacia, Universidad de La Laguna, 38071, La Laguna, Tenerife, Spain.
Ruíz
T
T
Rodríguez
L
L
D002176:Candida albicans / Q000235:genetics*; D003001:Cloning, Molecular; D006597:Fructose-Bisphosphatase / Q000235:genetics*; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014170:Transformation, Genetic
D006597:Fructose-Bisphosphatase