title: Crystallization and preliminary X-ray analysis of endopolygalacturonase SE1 from Trichosporon penicillatum.
abstract: Endopolygalacturonase SE1 from Trichosporon penicillatum has been crystallized by the hanging-drop method of vapour diffusion using ammonium sulfate as a precipitant. The crystals belong to the hexagonal space group P6(1) or P6(5), with unit-cell parameters a = b = 135.0, c = 70.7 A, gamma = 120 degrees. The calculated V(M) based on one molecule per asymmetric unit is 3.09 A(3) Da(-1). A native data set from a crystal has been collected to 2.0 A resolution on a Cu Kalpha rotating-anode X-ray source.
authors.affiliation: Research Institute for Advanced Science and Technology, Osaka Prefecture University, Sakai, Osaka 599-8570, Japan.
authors.forename: C T
authors.initials: CT
authors.lastname: Lu
authors.forename: T
authors.initials: T
authors.lastname: Tada
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: K
authors.initials: K
authors.lastname: Wada
authors.forename: K
authors.initials: K
authors.lastname: Nishimura
authors.forename: Y
authors.initials: Y
authors.lastname: Katsuya
authors.forename: M
authors.initials: M
authors.lastname: Sawada
authors.forename: M
authors.initials: M
authors.lastname: Takao
authors.forename: T
authors.initials: T
authors.lastname: Sakai
mesh_terms: D018360:Crystallography, X-Ray; D011096:Polygalacturonase / Q000737:chemistry* / Q000302:isolation & purification; D011487:Protein Conformation; D014250:Trichosporon / Q000201:enzymology*
chemical_list: C418471:endopolygalacturonase SE1; D011096:Polygalacturonase