title: Isolation of Xenopus frizzled-10A and frizzled-10B genomic clones and their expression in adult tissues and embryos.
abstract: Frizzled genes, encoding WNT receptors, play key roles in cell fate determination. Here, we isolated two Xenopus frizzled genes (Xfz10A and Xfz10B), probably reflecting pseudotetraploidy in Xenopus. Xfz10A (586 amino acids) and Xfz10B (580 amino acids) both encoded by a single exon, consisted of the N-terminal cysteine-rich domain, seven transmembrane domains, and the C-terminal Ser/Thr-X-Val motif. Xfz10A and Xfz10B were 97.0% identical at the amino acid level, and Xfz10B was 100% identical to previously reported Xfz9, yet Xfz10A was 85.3% and 62.4% identical to FZD10 and FZD9, respectively. Xfz10 mRNA appeared as 3.4 kb in adult tissues and embryos. RT-PCR analyses revealed the expression of more Xfz10A mRNA in stomach, kidney, eye, skeletal muscle, and skin, and more Xfz10B mRNA in heart and ovary, but in embryos, two mRNAs were equally expressed from the blastula stage with their peak expression at the late gastrula stage. The main site of Xfz10 mRNA expression was neural fold at the neurula stage and the dorsal region of midbrain, hindbrain, and spinal cord at the tadpole stage. These results suggest that Xfz10 has important roles in neural tissue formation.
authors.affiliation: Laboratory of Molecular Embryology, Department of Integrated Biosciences, Graduate School of Frontier Science, University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo, 113-0033, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Moriwaki
authors.forename: E
authors.initials: E
authors.lastname: Kajita
authors.forename: H
authors.initials: H
authors.lastname: Kirikoshi
authors.forename: J
authors.initials: J
authors.lastname: Koike
authors.forename: N
authors.initials: N
authors.lastname: Sagara
authors.forename: Y
authors.initials: Y
authors.lastname: Yasuhiko
authors.forename: T
authors.initials: T
authors.lastname: Saitoh
authors.forename: M
authors.initials: M
authors.lastname: Hirai
authors.forename: M
authors.initials: M
authors.lastname: Katoh
authors.forename: K
authors.initials: K
authors.lastname: Shiokawa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D051157:Frizzled Receptors; D018507:Gene Expression Regulation, Developmental; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000235:genetics; D043562:Receptors, G-Protein-Coupled; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014981:Xenopus; D029867:Xenopus Proteins
chemical_list: D051157:Frizzled Receptors; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D029867:Xenopus Proteins; C492385:fzd10a protein, Xenopus; C492384:fzd10b protein, Xenopus; D004247:DNA