A simple physiologic pulsatile perfusion system for the study of intact vascular tissue.
Perfusion vascular culture models may provide a useful link between cell culture models and animal culture models by allowing a high level of control over important parameters while maintaining physiologic structure. The purpose of this study was to develop and test a new vascular culture system for pulsatile perfusion culture of intact vascular tissue. The system generates a pulsatile component of flow by means of a cam-driven syringe and a peristaltic pump and compliance chamber. Cams were designed, constructed and tested to simulate canine femoral and common carotid artery flows. The mean pressure was adjusted between 60 and 200 mmHg without significantly affecting flow rate, flow waveform, or the pressure waveform. Porcine common carotid artery segments were cultured in this pulsatile perfusion system. The viability of vascular segments was tested after various culture times with a functional assay that demonstrated both smooth muscle cell and endothelial cell response to vasomotor challenge.
Conklin
B S
BS
Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA.
Surowiec
S M
SM
Lin
P H
PH
Chen
C
C
D000818:Animals; D001783:Blood Flow Velocity / Q000502:physiology; D001794:Blood Pressure / Q000502:physiology; D017536:Carotid Artery, Common / Q000166:cytology / Q000502:physiology*; D002470:Cell Survival; D046508:Culture Techniques / Q000295:instrumentation* / Q000379:methods; D004285:Dogs; D004730:Endothelium, Vascular / Q000166:cytology; D005260:Female; D005263:Femoral Artery / Q000502:physiology; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D011673:Pulsatile Flow / Q000502:physiology*; D013552:Swine