Molecular cloning and DNA analysis of the orotidine-5'-phosphate decarboxylase gene from the yeast Saccharomyces exiguus Yp74L-3.
The orotidine-5'-phosphate decarboxylase gene of Saccharomyces exiguus Yp74L-3 was cloned as a DNA fragment complementing a ura4 mutation of this yeast. The coding region of the gene is 807 bp in length, and represents 68.7% similarity to the corresponding gene of S. cerevisiae (URA3). The cloned URA4 gene was shown to be located on the 790-kbp Chromosome (chr) VIII of S. exiguus Yp74L-3. The neighbor-joining phylogenetic tree based on the orotidine-5'-phosphate decarboxylase coding sequences indicates that S. exiguus Yp74L-3 is closely related to Kluyveromyces yeasts, as well as to a S. cerevisiae laboratory strain.
Hisatomi
T
T
Department of Biotechnology, Faculty of Engineering, Fukuyama University, Gakuen-cho, Fukuyama, Hiroshima 729-0292, Japan. hisatomi@bt.fubt.fukuyama-u.ac.jp
Kodama
T
T
Toba
H
H
Moto-oka
M
M
Watanabe
A
A
Tsuboi
M
M
D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D003001:Cloning, Molecular; D004271:DNA, Fungal; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000235:genetics*; D010802:Phylogeny; D012440:Saccharomyces / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D017422:Sequence Analysis, DNA
D004271:DNA, Fungal; D009964:Orotidine-5'-Phosphate Decarboxylase