Utilization of dimethyl sulfide as a sulfur source with the aid of light by Marinobacterium sp. strain DMS-S1.
Strain DMS-S1 isolated from seawater was able to utilize dimethyl sulfide (DMS) as a sulfur source only in the presence of light in a sulfur-lacking medium. Phylogenetic analysis based on 16S ribosomal DNA genes indicated that the strain was closely related to Marinobacterium georgiense. The strain produced dimethyl sulfoxide (DMSO), which was a main metabolite, and small amounts of formate and formaldehyde when grown on DMS as the sole sulfur source. The cells of the strain grown with succinate as a carbon source were able to use methyl mercaptan or methanesulfonate besides DMS but not DMSO or dimethyl sulfone as a sole sulfur source. DMS was transformed to DMSO primarily at wavelengths between 380 and 480 nm by heat-stable photosensitizers released by the strain. DMS was also degraded to formaldehyde in the presence of light by unidentified heat-stable factors released by the strain, and it appeared that strain DMS-S1 used the degradation products, which should be sulfite, sulfate, or methanesulfonate, as sulfur sources.
Fuse
H
H
Chugoku National Industrial Research Institute, 2-2-2 Hirosuehiro, Kure, Hiroshima 737-0197, Japan. fuse@cniri.go.jp
Takimura
O
O
Murakami
K
K
Yamaoka
Y
Y
Omori
T
T
D001673:Biodegradation, Environmental; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D020563:Gammaproteobacteria / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D005798:Genes, Bacterial; D008027:Light; D008954:Models, Biological; D012623:Seawater / Q000382:microbiology; D013440:Sulfides / Q000378:metabolism*; D013455:Sulfur / Q000378:metabolism
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D013440:Sulfides; D013455:Sulfur; C004784:dimethyl sulfide