Development of shuttle vectors for spirochetes.
Constructions of Escherichia coli-spirochete shuttle vectors are based on naturally occurring plasmids, broad host range plasmids or bacteriophages. This review primarily focuses on genetic tools for Treponema denticola which is associated with periodontal diseases. The T. pallidum FlaA protein, E. coli beta-galactosidase, and the green fluorescent protein were successfully expressed in T. denticola from a shuttle vector system.
Girons
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Unité de Bactériologie Moléculaire et Médicale, Institut Pasteur, Paris, France. isgirons@pasteur.fr
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Kuramitsu
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D005408:Flagellin / Q000235:genetics; D017930:Genes, Reporter; D005822:Genetic Vectors; D006801:Humans; D018741:Replication Origin; D013144:Spirochaetales / Q000235:genetics*; D013145:Spirochaetales Infections / Q000382:microbiology; D014208:Treponema / Q000235:genetics
D005408:Flagellin; C067138:flaA protein, bacteria