Cloning, mapping, and expression analysis of a gene encoding a novel mammalian EGF-related protein (SCUBE1).
The epidermal growth factor (EGF) superfamily comprises a diverse group of proteins that function as secreted signaling molecules, growth factors, and components of the extracellular matrix, many with a role in vertebrate development. We have isolated a novel mammalian gene encoding an EGF-related protein with a CUB (C1s-like) domain that defines a new mammalian gene family. The Scube1 (signal peptide-CUB domain-EGF-related 1) gene was isolated from a developing mouse urogenital ridge cDNA library and is expressed prominently in the developing gonad, nervous system, somites, surface ectoderm, and limb buds. We have mapped Scube1 to mouse chromosome 15 and show that it is orthologous to a human gene in the syntenic region of chromosome 22q13. We discuss the possible functions of this novel gene and its role in heritable disease in light of these data.
Grimmond
S
S
MRC Mammalian Genetics Unit, Harwell, OX11 0RD, United Kingdom.
Larder
R
R
Van Hateren
N
N
Siggers
P
P
Hulsebos
T J
TJ
Arkell
R
R
Greenfield
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001254:Astrocytoma / Q000235:genetics; D002135:Calcium-Binding Proteins; D002874:Chromosome Mapping; D002892:Chromosomes, Human, Pair 22; D003001:Cloning, Molecular; D004622:Embryo, Mammalian; D004815:Epidermal Growth Factor / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D051379:Mice; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D002135:Calcium-Binding Proteins; D036341:Intercellular Signaling Peptides and Proteins; C417547:Scube1 protein, mouse; D004815:Epidermal Growth Factor