title: Delayed haemolytic activity by the freshwater puffer Tetraodon sp. toxin.
abstract: In order to elucidate the toxin composition of the freshwater puffer in Bangladesh, about 230 specimens of Tetraodon sp. were collected from 1997 to 1999 and extracted. After partitioning the toxins between an aqueous layer and a 1-butanol layer, the toxin in the aqueous layer was characterized as paralytic shellfish poison (PSP) (data not shown), while the toxin in the 1-butanol layer was identified as palytoxin (PTX) or PTX-like substance based on the delayed haemolytic activity which was inhibited by an anti-PTX antibody and ouabain (g-strophanthin). This is the first report on the occurrence of PTX or PTX-like substance(s) in puffer fish.
authors.affiliation: Graduate School of Science and Technology, Nagasaki University, 1-14, Bunkyo-machi, 852-8021, Nagasaki, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Taniyama
authors.forename: Y
authors.initials: Y
authors.lastname: Mahmud
authors.forename: M B
authors.initials: MB
authors.lastname: Tanu
authors.forename: T
authors.initials: T
authors.lastname: Takatani
authors.forename: O
authors.initials: O
authors.lastname: Arakawa
authors.forename: T
authors.initials: T
authors.lastname: Noguchi
mesh_terms: D000178:Acrylamides / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000633:toxicity*; D000818:Animals; D001459:Bangladesh; D002851:Chromatography, High Pressure Liquid; D003064:Cnidarian Venoms; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005397:Fish Venoms / Q000633:toxicity*; D005400:Fishes, Poisonous; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D051379:Mice; D010042:Ouabain / Q000494:pharmacology; D013779:Tetrodotoxin / Q000037:antagonists & inhibitors / Q000633:toxicity*; D000095702:Polyether Toxins
chemical_list: D000178:Acrylamides; D003064:Cnidarian Venoms; D004791:Enzyme Inhibitors; D005397:Fish Venoms; D010042:Ouabain; D013779:Tetrodotoxin; C010272:palytoxin; D000095702:Polyether Toxins