title: Possible role of ascorbic acid in the oxidative damage induced by inhaled crystalline silica particles.
abstract: The selective interaction of ascorbic acid with crystalline silica (quartz) has been studied by measuring the ascorbic acid consumption (by means of UV/vis and IR spectroscopy) and the release of silicon when quartz particles or amorphous silica (Aerosil 50) is incubated in ascorbic acid solution. At a physiological ascorbic acid concentration, quartz, and not amorphous silica, reacts, suggesting the formation of a 1:1 silicon-ascorbate complex, while at higher concentrations, the reacting amount of ascorbic acid exceeds the amount of silicon that is released. Silicon tetrahedra bearing free silanols at the quartz surface are selectively attached by ascorbic acid. The particle-derived hydroxyl radical yield in the presence of hydrogen peroxide is increased on ascorbic acid-treated quartz in comparison with the original sample. The results presented herein are relevant because the depletion of ascorbic acid from the lung lining layer and the increased potential in particle-derived free radical generation may both contribute to the oxidative damage following inhalation of crystalline silica.
authors.affiliation: Dipartimento di Chimica IFM, Università di Torino, via Pietro Giuria 7, 10125 Torino, Italy.
authors.forename: I
authors.initials: I
authors.lastname: Fenoglio
authors.forename: G
authors.initials: G
authors.lastname: Martra
authors.forename: S
authors.initials: S
authors.lastname: Coluccia
authors.forename: B
authors.initials: B
authors.lastname: Fubini
mesh_terms: D001205:Ascorbic Acid / Q000737:chemistry*; D004391:Dust / Q000009:adverse effects; D005609:Free Radicals / Q000737:chemistry; D019570:Inhalation Exposure / Q000009:adverse effects; D010084:Oxidation-Reduction; D011791:Quartz / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D012995:Solubility; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
chemical_list: D004391:Dust; D005609:Free Radicals; D011791:Quartz; D012822:Silicon Dioxide; D001205:Ascorbic Acid