Analysis of intestinal adaptation gene expression by cDNA expression arrays.
BACKGROUND
As a tool for determining gene expression on a genomic scale, cDNA microarrays are a promising new technology that can be applied to the study of complex physiologic processes. The objective of this study was to characterize the expression of individual genes and patterns of gene expression that might provide insight into the mechanism of intestinal adaptation after massive small bowel resection.


METHODS
Male ICR mice underwent a 50% proximal small bowel resection (SBR) or sham operation. After 3 days, the remnant ileum was harvested, weighed, and RNA extracted. Changes in gene expression were detected utilizing Clontech Atlas mouse cDNA expression arrays. Some of these changes were confirmed by reverse transcriptase-polymerase chain reactions (RT-PCR) and Northern blots.


RESULTS
Analysis of these cDNA arrays revealed changes in the expression of multiple genes, including those involved in cell cycle regulation, apoptosis, DNA synthesis, and transcriptional regulation. The patterns of expression were consistent with the increased cell proliferation and apoptosis observed during intestinal adaptation. A large number of genes not previously associated with intestinal adaptation were identified.


CONCLUSIONS
This technology may facilitate the elucidation of the intricate cellular mechanisms underlying intestinal adaptation.
Erwin
C R
CR
Children's Hospital Medical Center, Department of Surgery, University of Cincinnati College of Medicine, Ohio 45229, USA. chris.erwin@chmcc.org
Falcone
R A
RA
Stern
L E
LE
Kemp
C J
CJ
Warner
B W
BW
D000220:Adaptation, Biological / Q000235:genetics* / Q000502:physiology; D000714:Anastomosis, Surgical; D000818:Animals; D015152:Blotting, Northern; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000379:methods*; D007421:Intestine, Small / Q000601:surgery; D007422:Intestines / Q000502:physiology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012333:RNA, Messenger; D011897:Random Allocation; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger