title: Correlation between ICAM-1 and functional recovery of piglet myocardium with leukocyte-depleted reperfusion.
abstract: BACKGROUND
  Reperfusion injury involves leukocyte-endothelial interaction mediated by cell adhesion molecules. This study was designed to determine the time course of intercellular adhesion molecule-1 (ICAM-1) expression and the functional recovery of myocardium when reperfused with leukocyte depleted whole blood.
  
  
  METHODS
  Sixteen neonatal piglet hearts were harvested and stored with 4 degrees C cold University of Wisconsin Solution (UWS) for 12 hours. An ex vivo model consisting of an isolated working heart perfusion circuit, roller pumps, and a membrane oxygenator, was used for reperfusion. Atrial tissues were taken for staining ICAM-1. The stroke work index (SWI) was calculated during 4 hours of reperfusion. Two groups (group 1: reperfused with whole blood, group 2: with leukocyte depleted blood) were compared.
  
  
  RESULTS
  The differences of ICAM-1 expression between group 1 and 2 were significant at 3 and 4 hours of reperfusion (p < 0.05). The differences of the mean stroke work indices were significant at 2, 3, and 4 hours after reperfusion (p < 0.05).
  
  
  CONCLUSIONS
  Leukocyte-depleted reperfusion attenuates the expression of ICAM-1 and reduces the time-dependent functional deterioration of the myocardium. These results suggest that adhesion molecule like ICAM-1 plays a major role in deteriorating myocardial function during the reperfusion, possibly by leukocyte-mediated inflammatory process.
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, and Pathology, Seoul National University Hospital, Seoul National University College of Medicine, Seoul National University Medical Research Center, Heart Research Institute, Korea. jrl@plaza.snu.ac.kr
authors.forename: J R
authors.initials: JR
authors.lastname: Lee
authors.forename: J J
authors.initials: JJ
authors.lastname: Han
authors.forename: J W
authors.initials: JW
authors.lastname: Seo
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D006321:Heart / Q000502:physiology*; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis* / Q000502:physiology; D007962:Leukocytes / Q000502:physiology*; D015425:Myocardial Reperfusion / Q000379:methods*; D009206:Myocardium / Q000378:metabolism; D013552:Swine; D013997:Time Factors
chemical_list: D018799:Intercellular Adhesion Molecule-1