title: Immunostimulating and protective effects of an oral polybacterial immunomodulator 'Dentavax' in a rabbit experimental model.
abstract: The immunostimulating and protective effects of an oral polybacterial immunomodulator, Dentavax (D), composed of killed cells from Klebsiella pneumoniae, Streptococcus pyogenes, Staphylococcus aureus, Candida albicans and Lactobacillus acidophilus and their lysates, have been investigated on an experimental rabbit model. In this model, mixed suspensions of the above bacterial wild strains have been injected in six sides of oral mucosa. A long-lasting inflammation with the development of infiltrates and confluating abscesses has been observed. The influence of orally given Dentavax on the course of the model infection as well as on the dynamics of the immune response has been studied. A two-fold decrease in the duration and severity of inflammatory reaction, confirmed by the histological findings, has been registered. In immunised animals, an activation of polymorphonuclear phagocytosis, together with stimulation of humoral systemic and mucosal immunity with synthesis of specific serum (predominantly, IgG) and coproantibodies (predominantly, S-IgA) determined by ELISA, has been found. The results obtained proved the strong immunostimulating and protective effects of the preparation D, which is meant for the prophylaxis and treatment of inflammatory periodontal diseases.
authors.affiliation: Head Laboratory of Humoral and Mucosal Immunity, National Center of Infectious and Parasitic Diseases, 26 Yanko Sakazov Blvd, 1504, Sofia, Bulgaria. ncipd@ncipd.netbg.com
authors.forename: S
authors.initials: S
authors.lastname: Marinova
authors.forename: L
authors.initials: L
authors.lastname: Tchorbadjiiska
authors.forename: B
authors.initials: B
authors.lastname: Petrunov
authors.forename: J
authors.initials: J
authors.lastname: Cvetanov
authors.forename: P
authors.initials: P
authors.lastname: Nenkov
authors.forename: D
authors.initials: D
authors.lastname: Konstantinova
authors.forename: R
authors.initials: R
authors.lastname: Markova
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000284:Administration, Oral; D000818:Animals; D001419:Bacteria / Q000276:immunology*; D018928:Immunity, Mucosal; D007114:Immunization; D007071:Immunoglobulin A, Secretory / Q000096:biosynthesis; D009061:Mouth Mucosa / Q000276:immunology; D009504:Neutrophils / Q000276:immunology; D010587:Phagocytosis; D011817:Rabbits
chemical_list: D000276:Adjuvants, Immunologic; D007071:Immunoglobulin A, Secretory