A simple technique for rapid colonization of Anopheles quadrimaculatus using adults aspirated from livestock barns.
A technique was developed for rapid colonization of Anopheles quadrimaculatus larvae in an improvised insectary using blood-fed mosquitoes aspirated from livestock barns. A novel device termed the mosquito aspiration transfer and ovipositional chamber (MATOC) is described. In 2 field seasons, 14 broods were successfully mass reared, yielding more than 28,500 vigorous 3rd- and 4th-stage larvae used in rice plot and other bioassays. Crowding the females over a natural ovipositional substrate induced oviposition as early as 12 h from introduction into the MATOCs.
Dennett
J A
JA
Department of Entomology, University of Arkansas, Fayetteville 72701, USA.
Meisch
M V
MV
D000818:Animals; D000829:Animals, Domestic / Q000469:parasitology; D000852:Anopheles; D005260:Female; D006799:Housing, Animal; D007814:Larva; D010058:Oviposition