Monoclonal antibody detection of naphthalene dioxygenase from Pseudomonas aeruginosa 2NR.
A monoclonal antibody, designated mAb alpha(CT), was generated against a peptide of the ISP(NAP) alpha-subunit of the naphthalene dioxygenase (NDO) enzyme of Pseudomonas aeruginosa. Since NDO expression is induced by aromatic hydrocarbons, its detection is important as a tool for environmental biomonitoring. This antibody is highly specific and works well both in an indirect ELISA assay and Western Blot analysis, allowing the detection of Pseudomonas spp. expressing the NDO inducible enzyme. The detection threshold for the ELISA assay developed in this work was 10(4) colony forming units (cfu) per ml. Thus, this mAb could represent a powerful tool to test for pollutants in soil, groundwater, and other natural environments.
Civilini
M
M
Dipartimento di Scienze degli Alimenti, Università degli Studi di Udine, Italy.
Pucillo
C
C
Colombatti
A
A
Damante
G
G
de Bertoldi
M
M
Tell
G
G
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity; D015153:Blotting, Western; D049308:Dioxygenases; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000032:analysis* / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D010105:Oxygenases / Q000032:analysis* / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000254:growth & development
D000911:Antibodies, Monoclonal; D009097:Multienzyme Complexes; D010105:Oxygenases; D049308:Dioxygenases; C038703:naphthalene dioxygenase