Fas activation reduces neutrophil adhesion to endothelial cells.
Polymorphonuclear neutrophils (PMN) express apoptotic markers and lose effector functions including adhesion, chemotaxis, and phagocytosis when cultured overnight. Although the loss of function correlates with apoptosis, it is not clear if functions are lost before an early marker of apoptosis, the display of phosphatidylserine (PS), targets PMN for removal by phagocytic cells. To address this question, freshly isolated PMN were treated with Fas-activating antibodies to induce apoptosis rapidly. Early markers of apoptosis and PMA-stimulated adhesion to endothelial cells were measured. After 1 h of Fas exposure, only 16% PMN had externalized PS. In contrast, Fas activation reduced PMA-stimulated adhesion between 68 and 27% depending on PMA concentration. The loss of adhesion was accompanied by a reduction in beta2 integrin expression and receptor clustering. These results indicate that the Fas-induced loss of adhesion may precede PS externalization and could limit participation in the inflammatory response before PS externalization targets PMN for removal.
Greenstein
S
S
Department of Pharmacology, Rush University, Chicago, Illinois 60612, USA.
Barnard
J
J
Zhou
K
K
Fong
M
M
Hendey
B
B
D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D018821:CD18 Antigens / Q000096:biosynthesis / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D004730:Endothelium, Vascular / Q000166:cytology* / Q000187:drug effects; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D006801:Humans; D009504:Neutrophils / Q000166:cytology* / Q000187:drug effects; D010718:Phosphatidylserines / Q000032:analysis / Q000378:metabolism; D051381:Rats; D017452:Receptors, IgG / Q000096:biosynthesis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D019014:fas Receptor / Q000276:immunology / Q000502:physiology*
D017304:Annexin A5; D000911:Antibodies, Monoclonal; D018821:CD18 Antigens; D005456:Fluorescent Dyes; D010718:Phosphatidylserines; D017452:Receptors, IgG; D019014:fas Receptor; D016650:Fluorescein-5-isothiocyanate; D013755:Tetradecanoylphorbol Acetate