title: Increase in blood pressure with enhanced Na+, K+ -ATPase activity in stroke-prone spontaneously hypertensive rats after 4-weeks intake of rapeseed oil as the sole dietary fat.
abstract: Twenty stroke-prone spontaneously hypertensive rats were divided into 2 groups of 10 animals each, and fed a defatted diet and orally administered rapeseed (canola) oil or soybean oil at 10 (w/w)% of the consumed diet once a day for 4 weeks. At the 4th week of administration, the systolic blood pressure in the canola oil group was higher (235 +/- 2 mmHg, mean +/- S.E.M., N=10) than that in the soybean oil group (225 +/- 4 mmHg, N=10, P<0.05). In isolated, perfused mesenteric bed from these rats, the increase in perfusion pressure by norepinephrine, ATP, arachidonic acid, endothelin-1, angiotensin II or serotonin showed no between-group differences. There were also no between-group differences in the production of thromboxane A2 and prostaglandin 12 in the outflow by arachidonic acid injection. On the other hand, in the isolated aortic ring from the canola oil group, developed tension in potassium-free solution was enhanced with activation of Na+, K+ -ATPase. These results suggest that canola oil intake as the sole dietary fat increases systolic blood pressure of stroke-prone spontaneously hypertensive rats. The changes in vascular responsiveness to vasoconstrictors and production of prostanoids are unlikely to have relevance to the elevation of blood pressure. However, altered Na+, K+ -ATPase activity may play a role in the promotion of blood pressure elevation.
authors.affiliation: Department of Pharmacology, Hatano Research Institute, Food and Drug Safety Center, Hadano, Kanagawa, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Naito
authors.forename: C
authors.initials: C
authors.lastname: Konishi
authors.forename: H
authors.initials: H
authors.lastname: Katsumura
authors.forename: N
authors.initials: N
authors.lastname: Ohara
mesh_terms: D015121:6-Ketoprostaglandin F1 alpha / Q000032:analysis; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects*; D004041:Dietary Fats / Q000008:administration & dosage*; D004797:Enzyme-Linked Immunosorbent Assay; D005229:Fatty Acids, Monounsaturated; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000201:enzymology*; D010938:Plant Oils / Q000008:administration & dosage / Q000494:pharmacology*; D000074262:Rapeseed Oil; D051381:Rats; D011918:Rats, Inbred SHR; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013929:Thromboxane B2 / Q000032:analysis
chemical_list: D004041:Dietary Fats; D005229:Fatty Acids, Monounsaturated; D010938:Plant Oils; D000074262:Rapeseed Oil; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D000254:Sodium-Potassium-Exchanging ATPase