[Thymidylate synthase and dihydropyrimidine dehydrogenase activity in a metastatic liver tumor from colorectal cancer].
The value of thymidylate synthase (TS) and dihydropyrimidine dehydrogenase (DPD) activity in metastatic liver tumor from colorectal cancer as predictive parameters for hepatic artery infusion (HAI) chemotherapy with 5-FU was investigated. Thirteen patients with metachronous liver metastases underwent hepatic resection and primary site colectomy. We measured TS and DPD activity in normal colonic mucosa, primary colon tumor, and metastatic liver tumor in these patients. The TS (pmol/g-tissue) of primary tumor, metastatic tumor, and normal colonic mucosa was 6.6 +/- 1.40 (mean +/- SE), 3.2 +/- 0.83 and 2.5 +/- 0.83 respectively. The DPD (pmol/min/mg/protein) of primary tumor, metastatic tumor, and normal colonic mucosa was 31.7 +/- 5.49, 82.1 +/- 12.5 and 50.6 +/- 8.46, respectively. There was significant relationship between DPD activity level and recurrence. It is suggested that 5-FU catabolism in metastatic liver tumor was increased, and DPD activity may be a valuable predictive marker for tumor response to HAI therapy.
Umekita
N
N
Dept. of Surgery, Tokyo Metropolitan Bokutou Hospital.
Tanaka
S
S
Abe
H
H
Kitamura
M
M
D000964:Antimetabolites, Antineoplastic / Q000627:therapeutic use; D003106:Colon / Q000201:enzymology; D015179:Colorectal Neoplasms / Q000473:pathology*; D042943:Dihydrouracil Dehydrogenase (NADP); D005472:Fluorouracil / Q000627:therapeutic use; D006801:Humans; D007261:Infusions, Intra-Arterial; D008113:Liver Neoplasms / Q000188:drug therapy / Q000201:enzymology* / Q000556:secondary*; D009703:Nucleoside-Phosphate Kinase / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism*
D000964:Antimetabolites, Antineoplastic; D010088:Oxidoreductases; D042943:Dihydrouracil Dehydrogenase (NADP); D009703:Nucleoside-Phosphate Kinase; C020093:dTMP kinase; D005472:Fluorouracil