Gene expression of GLUT isoforms and VHL in oral squamous cell carcinoma.
In human oral squamous cell carcinoma (OSCC) cell lines, we detected atypical mRNA expression of GLUT2 and/or GLUT4 in addition to enhanced expression of GLUT1 mRNA using RT-PCR. In semi-quantitative reverse transcription-polymerase chain reaction analysis of mRNA expression in OSCC cell lines, we found an inverse relationship between mRNA expression of von Hippel-Lindau (VHL) and that of GLUT1, with no apparent influence on the expression of other GLUTs. These findings suggest that the reduction of VHL may play a critical role in glucose uptake of OSCC cell lines, with enhancement of GLUT1 expression.
Fukuzumi
M
M
Department of Oral and Maxillofacial Surgery, Ehime University School of Medicine, Shitsukawa, Shigenobu-cho, Onsengun, 791-0295, Ehime, Japan. fukuzumi@m.ehime-u.ac.jp
Hamakawa
H
H
Onishi
A
A
Sumida
T
T
Tanioka
H
H
D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D002478:Cells, Cultured; D015536:Down-Regulation; D015870:Gene Expression; D005947:Glucose / Q000493:pharmacokinetics; D051272:Glucose Transporter Type 1; D051273:Glucose Transporter Type 2; D051274:Glucose Transporter Type 3; D051275:Glucose Transporter Type 4; D006801:Humans; D008025:Ligases; D009004:Monosaccharide Transport Proteins / Q000096:biosynthesis / Q000737:chemistry*; D009062:Mouth Neoplasms / Q000378:metabolism*; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; D014176:Protein Biosynthesis; D020033:Protein Isoforms; D011506:Proteins / Q000737:chemistry*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins; D044767:Ubiquitin-Protein Ligases; D015854:Up-Regulation; D051794:Von Hippel-Lindau Tumor Suppressor Protein
D051272:Glucose Transporter Type 1; D051273:Glucose Transporter Type 2; D051274:Glucose Transporter Type 3; D051275:Glucose Transporter Type 4; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; D020033:Protein Isoforms; D011506:Proteins; D012333:RNA, Messenger; C495677:SLC2A1 protein, human; C495687:SLC2A3 protein, human; C495692:SLC2A4 protein, human; D025521:Tumor Suppressor Proteins; D044767:Ubiquitin-Protein Ligases; D051794:Von Hippel-Lindau Tumor Suppressor Protein; D008025:Ligases; C497446:VHL protein, human; D005947:Glucose