title: Dynamic cell cycle kinetics in vitro and in vivo in myelodysplastic syndromes with special reference to the influence of hematopoietic growth factors.
abstract: We have investigated the effect of HGF in vivo and in vitro in MDS using a recently developed FCM assay involving the simultaneous measurement of cell surface antigens, DNA content, and BrdUrd or IodUrd incorporation. This allows for the determination of the dynamic cell kinetic parameters: LI, T(s), and T(pot) and we observed that in vitro HGF stimulation resulted in a significant decrease in mean T(pot) values from 6.6 to 3.5 days. Importantly, we demonstrated that in vivo GM-CSF administration to patients with RAEB resulted in a shortening of T(pot) within the 2 first weeks of GM-CSF treatment.
authors.affiliation: Department of Hematology, Immunhaematologisk Laboratorium, Arhus amtssygehus, Tage-Hansens Gade 2, Opgang 4A, DK 8000, Arhus, Denmark. andholm@post11.tele.dk
authors.forename: M
authors.initials: M
authors.lastname: Holm
authors.forename: M
authors.initials: M
authors.lastname: Hoyer
authors.forename: I
authors.initials: I
authors.lastname: Jensen
authors.forename: M
authors.initials: M
authors.lastname: Thomsen
authors.forename: P
authors.initials: P
authors.lastname: Hokland
mesh_terms: D000368:Aged; D015703:Antigens, CD; D001854:Bone Marrow Cells / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects*; D004247:DNA / Q000032:analysis; D005260:Female; D005434:Flow Cytometry; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology / Q000627:therapeutic use*; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology*; D006801:Humans; D016130:Immunophenotyping; D007963:Leukocytes, Mononuclear / Q000473:pathology; D019891:Leukopoiesis; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics / Q000473:pathology* / Q000628:therapy*; D014407:Tumor Cells, Cultured
chemical_list: D015703:Antigens, CD; D016298:Hematopoietic Cell Growth Factors; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D004247:DNA