Multicolor spectral karyotype analysis of a transplantable human ileal carcinoid.
In this report we present the results of a combined cytogenetic and multicolor spectral karyotype (SKY) analysis of a transplantable human ileal carcinoid (GOT1). By using SKY it was possible to identify the origin and organization of all clonal marker chromosomes and to identify cryptic translocations not detectable by conventional chromosome banding. The stemline karyotype of low passage GOT1 cells was interpreted as 43,XX, der(1)del(1)(?), inv(2)(p25q13), del(3)(p21), del(5)(q13q31), del(6)(q13), -9, -13, -15, del(16) (q22). Analysis of the GOT1 cells after about 2.5 years of propagation in nude mice allowed us to follow the in vivo progression of this tumor. Relatively few additional rearrangements had occurred during this period, indicating that the GOT1 cells are genetically stable. Most of the abnormalities detected result in loss of whole or parts of chromosomes, suggesting that loss of multiple chromosomal regions, presumably containing tumor suppressor genes, might be important genetic events in ileal carcinoids.
Sjögren
H
H
Department of Pathology, Lundberg Laboratory for Cancer Research, Goteborg University, Sahlgrenska University Hospital, Goteborg, Sweden.
Nilsson
O
O
Behrendt
M
M
Kölby
L
L
Jacobsen Levin
A M
AM
Ahlman
H
H
Stenman
G
G
D000818:Animals; D002276:Carcinoid Tumor / Q000235:genetics* / Q000473:pathology; D007446:Chromosome Inversion; D020223:Chromosome Painting / Q000379:methods*; D005260:Female; D006801:Humans; D007078:Ileal Neoplasms / Q000235:genetics* / Q000473:pathology; D007621:Karyotyping; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D009368:Neoplasm Transplantation; D014178:Translocation, Genetic; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured