title: Molecular cloning and phylogenetic analysis of canine beta-casein.
abstract: A canine beta-casein cDNA was isolated from mammary tissue by polymerase chain reaction (PCR) using degenerate primers. It encodes 250 amino acids protein containing the conserved sequence motif of beta-casein. It showed the highest homology with snow-leopard (Uncia uncia (55-62% identity). It also showed 44-53% identity with human, 33-42%, identity with mouse, 29-37%, identity with rat, 43-53% identity with rabbit, 41-48% identity with pig, 44-51% identity with cattle and 44-50% identity with sheep. A 1.2-kb mRNA was detected in mammary tissue by Northern blot analysis. Phylogenetic analysis revealed that canine beta-casein formed a branch with lesser panda and snow leopard, which were grouped into carnivore.
authors.affiliation: Department of Veterinary Pathology, Faculty of Agriculture, Miyazaki University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Watanabe
authors.forename: S
authors.initials: S
authors.lastname: Sugano
authors.forename: T
authors.initials: T
authors.lastname: Togashi
authors.forename: J
authors.initials: J
authors.lastname: Imai
authors.forename: K
authors.initials: K
authors.lastname: Uchida
authors.forename: R
authors.initials: R
authors.lastname: Yamaguchi
authors.forename: S
authors.initials: S
authors.lastname: Tateyama
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002020:Buffaloes; D002335:Carnivora; D002364:Caseins / Q000737:chemistry / Q000235:genetics*; D002417:Cattle; D003001:Cloning, Molecular; D004285:Dogs / Q000235:genetics*; D005260:Female; D006041:Goats; D006801:Humans; D008321:Mammary Glands, Animal / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D011817:Rabbits; D051381:Rats; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012756:Sheep; D013552:Swine
chemical_list: D002364:Caseins