Trp12, a novel Trp related protein from kidney.
A novel member of the transient receptor potential (Trp) family of ion channels, Trp12, was identified. The Trp12 mRNA is abundantly expressed in mouse kidney and encodes a protein of 871 amino acid residues. Trp12 transfected cells reveal an elevated cytosolic Ca(2+) and respond with a further increase of cytosolic Ca(2+) to perfusion with hypoosmotic solutions. The human orthologue of murine Trp12 was localized on a genomic clone derived from human chromosome 12. It is composed of 15 translated exons. The intron placement within that primary structure does not correlate with the previously postulated splice sites in transcripts encoding the stretch-inhibitable channel which shares a high degree of amino acid sequence identity with Trp12 and the vanilloid receptor type 1.
Wissenbach
U
U
Institut für Pharmakologie und Toxikologie der Universität des Saarlandes, D-66421 Homburg, Germany. ulrich.wissenbach@med-rz.uni-saarland.de
Bödding
M
M
Freichel
M
M
Flockerzi
V
V
D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D002460:Cell Line; D002881:Chromosomes, Human, Pair 12; D003001:Cloning, Molecular; D003062:Codon; D006224:Cricetinae; D003600:Cytosol / Q000378:metabolism; D004247:DNA / Q000032:analysis; D005091:Exons; D015870:Gene Expression; D006801:Humans; D007038:Hypotonic Solutions / Q000494:pharmacology; D007438:Introns; D007668:Kidney / Q000737:chemistry*; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012326:RNA Splicing; D012333:RNA, Messenger / Q000032:analysis; D017385:Sequence Homology; D050052:TRPC Cation Channels; D014162:Transfection
D015220:Calcium Channels; D003062:Codon; D007038:Hypotonic Solutions; D012333:RNA, Messenger; D050052:TRPC Cation Channels; C096351:transient receptor potential cation channel, subfamily C, member 1; D004247:DNA; D002118:Calcium