The yeast two-hybrid system and its pharmaceutical significance.
The detected phenotypes in many diseases are caused from dysfunction in protein-protein, protein-DNA and receptor-ligand interactions. Therefore, determination of these molecular interactions followed by designing or screening the compounds to target these interactions provides a significant challenge in drug development. This review aims to highlight the yeast two-hybrid system in determination of protein-protein interactions and its possible outcomes in pharmaceutical research. The variations of the basic methodology as one- and three-hybrid systems are also discussed in relation to their potential pharmaceutical applications.
Topcu
Z
Z
Department of Pharmaceutical Biotechnology, School of Pharmacy, Ege University, Izmir, Turkey. ztopcu@bornova.ege.edu.tr
Borden
K L
KL
D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015195:Drug Design; D017930:Genes, Reporter / Q000235:genetics*; D006801:Humans; D011485:Protein Binding / Q000235:genetics; D020798:Two-Hybrid System Techniques; D015003:Yeasts / Q000235:genetics*
D004268:DNA-Binding Proteins