Transcriptional activation analysis using bioluminescent reporter assays.
Analysis of promoter and enhancer DNA sequences provides the researcher with valuable information regarding the expression patterns of genes. Insertion of small DNA fragments containing the regulatory sequence of interest into vectors carrying reporter genes allows for the accurate quantitative analysis of the gene's expression patterns and responses to various stimuli. The use of bioluminescent reporter genes provides a simple, rapid, and inexpensive system that generates virtually no toxic or radioactive waste products. In addition, bioluminescent reporter vectors are more sensitive than previous methods such as the chloramphenicol acetyl transferase (CAT) systems, that require the use of hazardous chemicals and isotopically labeled reagents.
Hodge
D R
DR
Laboratory for Molecular Immunoregulation, NCI-FCRDC-NIH, Frederick, MD, USA. hodge@ncifcrf.gov
Clausen
P A
PA
D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D018274:Electroporation; D004742:Enhancer Elements, Genetic; D017930:Genes, Reporter; D005822:Genetic Vectors; D006801:Humans; D019169:Jurkat Cells; D008156:Luciferases / Q000032:analysis / Q000235:genetics*; D008163:Luminescent Measurements; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis; D013601:T-Lymphocytes; D015533:Transcriptional Activation; D014162:Transfection / Q000379:methods*
D011994:Recombinant Proteins; D008156:Luciferases