Ferrocene-Mediated carbon paste electrode modified with D-fructose dehydrogenase for batch mode measurement of D-fructose.
A mediated modified carbon paste and renewable surface electrode for fructose amperometric measurement based on D-fructose dehydrogenase (FDH) was prepared and optimized. Commercially available ferrocene (FcH) and hydroxymethyl ferrocene (FcCH2OH) were used as mediators. The substituted FcH showed better linearity and higher sensitivity. The influence of different experimental parameters was studied for optimum analytical performance. The final FDH-modified electrode showed good analytical performance for batch mode measurements of fructose.
Boujtita
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M
Laboratoire d'Analyse Isotopique et Electrochimique de Métabolismes (CNRS UPRSA 6006), Faculté des Sciences et des Techniques, Nantes, France.
El Murr
N
N
D001628:Beverages / Q000032:analysis*; D002138:Calibration; D002237:Carbohydrate Dehydrogenases / Q000378:metabolism; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000378:metabolism; D005296:Ferrous Compounds; D005632:Fructose / Q000032:analysis*; D005638:Fruit; D007700:Kinetics; D000075163:Metallocenes
D004800:Enzymes, Immobilized; D005296:Ferrous Compounds; D000075163:Metallocenes; D005632:Fructose; D002237:Carbohydrate Dehydrogenases; C028761:D-fructose 5-dehydrogenase; C004998:ferrocene