An efficient solid-phase strategy for the construction of chemokines.
Synthesis of chemokines via stepwise SPPS approaches has been shown to be a challenge. Herein, a complete study of different coupling methods, solvents and temperature combined with a continuous-flow synthesizer equipped with feedback monitoring was carried out. The results from this study indicate that this family of molecules can be prepared using an Fmoc/Bu(t) chemical approach and provide a general method to apply for the elongation of other difficult sequences.
McNamara
J F
JF
PerSeptive Biosystems Inc., Framingham, MA, USA.
Lombardo
H
H
Pillai
S K
SK
Jensen
I
I
Albericio
F
F
Kates
S A
SA
D000595:Amino Acid Sequence; D001671:Biochemistry / Q000379:methods*; D018946:Chemokine CCL5 / Q000138:chemical synthesis; D018925:Chemokines / Q000138:chemical synthesis*; D008969:Molecular Sequence Data
D018946:Chemokine CCL5; D018925:Chemokines