Some oncogene and tumour suppressor gene protein products expression in B-cell chronic lymphocytic leukaemia.
The expression of Bcl-2, P53 proteins and known markers of proliferation, namely proliferating cell nuclear antigen (PCNA) and Ki67, in 29 patients with B-cell chronic lymphocytic leukaemia (B-CLL) was investigated. All leukaemic patients were classified, and immunophenotyped by the two-colour immunofluorescence method with the use of fluorocytometry. B-CLL was heterogeneous in the range of biological parameters of tumour cells. B-CLL patients manifested 34% positive Ki67 and 61% PCNA expression, whereas Bcl-2 and P53 positivity was 81% and 42%, respectively. The level of intracellular expression of Bcl-2 and P53 proteins did not depend on the stage of disease estimated by routine methods. Ki67 and PCNA expression was significantly higher in B-CLL patients with more advanced stages of the disease. A statistically significant correlation was established between their mutual expression.
Niewiadomska
H
H
Department of Oncology, Medical University of Lódź, Poland.
Mirowski
M
M
Kulczycka
D
D
Najder
M
M
Balcerczak
E
E
Błoński
J Z
JZ
D000368:Aged; D000369:Aged, 80 and over; D002452:Cell Count; D005260:Female; D005434:Flow Cytometry; D016147:Genes, Tumor Suppressor / Q000502:physiology*; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009857:Oncogenes / Q000502:physiology*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53