A preliminary evaluation of intra- and interindividual variations of hOGG1 messenger RNA levels in peripheral blood cells as determined by a real-time polymerase chain reaction technique.
The hOGG1 (8-oxoguanine-DNA glycosylase) gene may contribute to further understanding of the relation between oxidative DNA damage and carcinogenesis. A real-time PCR technique was used to determine mRNA levels in peripheral blood cells to assess the possibility of using hOGG1 mRNA as a biomarker in epidemiological studies. To evaluate the intra- and interindividual variation of hOGG1 mRNA levels in peripheral blood cells, we measured them in five healthy nonsmokers three times over a 1-year period. The beta-actin mRNA level in each subject was set equal to 100, and the levels of hOGG1 mRNA were found to range from 1.6-17.6. The intraindividual variation range was 1.8-6.4. Although the difference in the mRNA levels between the sampling dates was not significant (P = 0.73), a significant difference in mRNA levels was found between the subjects (P < 0.01). The subjects seemed to fall into groups according to their individual levels. This preliminary study may provide initial information on the hOGG1 mRNA level of peripheral blood cells as a biomarker in epidemiological studies on oxygen radicals, oxygen radical-related agents, and cancer.
Hanaoka
T
T
Epidemiology and Biostatistics Division, National Cancer Center Research Institute East, Chiba, Japan.
Yamano
Y
Y
Hashimoto
H
H
Kagawa
J
J
Tsugane
S
S
D000328:Adult; D014408:Biomarkers, Tumor / Q000032:analysis*; D002471:Cell Transformation, Neoplastic; D004249:DNA Damage; D043382:DNA-Formamidopyrimidine Glycosylase; D016021:Epidemiologic Studies; D005609:Free Radicals; D014644:Genetic Variation; D006801:Humans; D009699:N-Glycosyl Hydrolases / Q000032:analysis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics*; D017382:Reactive Oxygen Species; D012016:Reference Values
D014408:Biomarkers, Tumor; D005609:Free Radicals; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D009699:N-Glycosyl Hydrolases; D043382:DNA-Formamidopyrimidine Glycosylase