title: Chemical and serological characterization of the Bordetella hinzii lipopolysaccharides.
abstract: Bordetella hinzii has recently been isolated from immunocompromised human hosts. The polysaccharides isolated from its endotoxin (lipopolysaccharide, LPS) were investigated using chemical analyses, NMR, gas-liquid chromatography/mass spectrometry and mass spectrometry by plasma desorption, matrix-assisted laser desorption/ionization and electrospray. The following structure for the O-chain-free LPS was deduced from the experimental results: carbohydrate structure [see text] Mass spectrometry and serology revealed that the O-chains were different from the homopolymer common to Bordetella bronchiseptica and Bordetella parapertussis strains and were composed of a trisaccharide repeating unit. Masses up to 8 kDa were obtained for native LPS molecular species.
authors.affiliation: Equipe Endotoxines, UMR 8619 du Centre National de la Recherche Scientifique, Biochimie, Université de Paris-Sud, Orsay, France.
authors.forename: L
authors.initials: L
authors.lastname: Aussel
authors.forename: R
authors.initials: R
authors.lastname: Chaby
authors.forename: K
authors.initials: K
authors.lastname: Le Blay
authors.forename: J
authors.initials: J
authors.lastname: Kelly
authors.forename: P
authors.initials: P
authors.lastname: Thibault
authors.forename: M B
authors.initials: MB
authors.lastname: Perry
authors.forename: M
authors.initials: M
authors.lastname: Caroff
mesh_terms: D000911:Antibodies, Monoclonal; D001884:Bordetella / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002855:Chromatography, Thin Layer; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D008401:Gas Chromatography-Mass Spectrometry; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000911:Antibodies, Monoclonal; D008070:Lipopolysaccharides; D009844:Oligosaccharides