title: Structure of mouse preproendothelin-3 and phylogenetic analysis of the endothelins.
abstract: Endothelin (ET)-related peptide hormones, ET-1, vasoactive intestinal contractor (VIC), ET-2 and ET-3, have multiple physiological roles including vasoconstriction. To reveal the structural diversity of the precursor proteins of the ET family, cDNAs, cross-hybridizing with ET-1 and VIC probes, were cloned from the mouse intestine library. The deduced protein is a 214-amino acid precursor of mouse ET-3, preproendothelin-3 (PPET-3), which is the counterpart of the hypothalamus-, not placenta-, derived human PPET-3. Sequence identities of PPET-3 amino acids of mouse with human and rat are 65% over 194 amino acids and 65% over 214 amino acids. Phylogenetic analysis of the precursor proteins for ET-1, VIC and ET-3 suggest that members of the ET family are distantly related and probably descended from a common ancestral gene.
authors.affiliation: National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology, Tsukuba, Ibaraki, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Saida
authors.forename: N
authors.initials: N
authors.lastname: Kometani
authors.forename: H
authors.initials: H
authors.lastname: Masuda
authors.forename: S
authors.initials: S
authors.lastname: Oka
authors.forename: T
authors.initials: T
authors.lastname: Uchide
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D019334:Endothelin-3 / Q000235:genetics; D016232:Endothelins / Q000737:chemistry* / Q000235:genetics*; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D011498:Protein Precursors / Q000737:chemistry*; D051381:Rats
chemical_list: D019334:Endothelin-3; D016232:Endothelins; D011498:Protein Precursors; C075625:preproendothelin-3