Determination of T-2 toxin in grain and grain products by HPLC and TLC.
The purpose of this study was to investigate the T-2 toxin contaminated grain and grain products consumed especially by Turkish population. The T-2 toxin was detected using the high performance liquid chromatography (HPLC) with UV detector at 208 nm and the identify of T-2 was further confirmed by thin layer chromatography (TLC). The recovery was 91 +/- 4.24% for corn flour fortified with the known amount of T-2 toxin (1 ppm). The detection limits of T-2 toxin for the HPLC and the TLC were 25 ng and 50 ng, respectively. A total of 30 commercially available grain and grain product samples were analyzed. Two corn flour samples were found to contain detectable levels of T-2 toxin at a level of 1.60 ppm and 4.08 ppm.
Omurtag
G Z
GZ
Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Marmara University, Haydarpaşa-Istanbul, Turkey.
Yazicioğlu
D
D
D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D002523:Edible Grain; D005506:Food Contamination; D013605:T-2 Toxin / Q000032:analysis*; D014421:Turkey
D013605:T-2 Toxin