Gonadotropin-releasing hormone fibers innervate the pituitary of the male African catfish (Clarias gariepinus) during puberty.
The development of the catfish gonadotropin-releasing hormone (cfGnRH) fiber network in the pituitary of male African catfish (Clarias gariepinus) was investigated in relation to puberty. Double immunolabeling studied by confocal laser scanning microscopy revealed a concomitant development of gonadotropes and of pituitary cfGnRH innervation during the first wave of spermatogenesis. Catfish GnRH-immunoreactive fibers in the proximal pars distalis (PPD) of the pituitary were initially observed at the age of 10 weeks (onset of spermatogonial proliferation) and gradually reached the adult pattern at the age of 20 weeks (spermatozoa present in the testis). The content of cfGnRH-associated peptide (cfGAP, part of the prohormone) in the pituitary similarly increased during puberty. At the electron microscopical level, fibers containing cfGAP-ir granules came into close proximity of the gonadotropes at 18 weeks of age. In vitro studies indicated a progressively increasing basal and cfGnRH-stimulated luteinizing hormone (LH) secretion during pubertal development. The LH secretion patterns were similar in response to exogenous cfGnRH (0.1 microM) or to endogenous cfGnRH, the release of which was induced by forskolin (1 microM). Castration experiments demonstrated that the innervation of the pituitary with cfGnRH fibers continued after surgery, accompanied by an increase in the cfGAP levels. However, gonadotrope development was retarded, suggesting a differential regulation of the two maturational processes. Since testosterone stimulates both processes, other testicular factors may also be involved. Puberty-associated changes in LH release patterns appear to reflect changes in the GnRH sensitivity and in the pool of releasable LH, while availability of cfGnRH does not appear to be a limiting factor.
Dubois
E A
EA
Graduate School for Developmental Biology, Research Group for Comparative Endocrinology, University of Utrecht, Faculty of Biology, Utrecht, The Netherlands. E.A.Dubois@bio.uu.nl
Zandbergen
M A
MA
Peute
J
J
Hassing
I
I
van Dijk
W
W
Schulz
R W
RW
Goos
H J
HJ
D000818:Animals; D002397:Catfishes / Q000033:anatomy & histology / Q000502:physiology*; D005576:Colforsin / Q000494:pharmacology; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000378:metabolism; D008297:Male; D008854:Microscopy, Electron; D009412:Nerve Fibers / Q000378:metabolism*; D009919:Orchiectomy; D010902:Pituitary Gland / Q000254:growth & development / Q000294:innervation* / Q000378:metabolism / Q000648:ultrastructure; D011498:Protein Precursors / Q000378:metabolism
D011498:Protein Precursors; C050502:gonadotropin releasing hormone associated peptide; D005576:Colforsin; D007987:Gonadotropin-Releasing Hormone; D007986:Luteinizing Hormone