title: Amino acid supplementation improves cell-specific functions of the rat hepatocytes exposed to human plasma.
abstract: Maintaining hepatocyte function during plasma exposure is critical for the successful development of hepatocyte-based bioartificial liver assist systems. Past attempts to culture hepatocytes in plasma yielded discouraging results. Using a stable culture model based on sandwiching hepatocytes between two layers of collagen gel, we investigated the effect of hormone and amino acid supplementation during exposure of rat hepatocytes to heparin-treated human plasma for 1 week. Morphology and hepatocyte-specific functions were evaluated for hepatocytes cultured in Dulbecco's Modified Eagle medium (DMEM), nonsupplemented plasma, plasma supplemented with hormones, or with hormones plus amino acids. Amino acids were supplemented at four-fold concentration of Basal Medium Eagle with 4 mM glutamine, whereas hormones included 7.5 microg/mL of hydrocortisone and 50 microU/mL of insulin. Cuboidal structure and bile canaliculi formation were observed throughout the 1-week exposure period for control hepatocytes in DMEM and for hepatocytes cultured in hormone supplemented plasma. Albumin and urea synthesis rates of hepatocytes in hormone plus amino acid supplemented plasma during the last day of plasma exposure were 60.4 +/- 13.7 and 75.6 +/- 6.5 (microg/day per 1 x 10(6) cells, mean +/- SD), respectively, comparable to cultures in standard culture medium. On the other hand, hepatocytes exposed to nonsupplemented plasma suffered significant morphological and functional damage. The results of this study indicate that hormone plus amino acid supplementation help to restore function in hepatocytes exposed to plasma.
authors.affiliation: Center for Engineering in Medicine and Surgical Services, Massachusetts General Hospital, Harvard Medical School, and Shriners Hospital for Children, Boston, Massachusetts 02114, USA.
authors.forename: J
authors.initials: J
authors.lastname: Washizu
authors.forename: C
authors.initials: C
authors.lastname: Chan
authors.forename: F
authors.initials: F
authors.lastname: Berthiaume
authors.forename: R G
authors.initials: RG
authors.lastname: Tompkins
authors.forename: M
authors.initials: M
authors.lastname: Toner
authors.forename: M L
authors.initials: ML
authors.lastname: Yarmush
mesh_terms: D000418:Albumins / Q000096:biosynthesis; D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D005260:Female; D005934:Glucagon / Q000494:pharmacology; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D011917:Rats, Inbred Lew; D014508:Urea / Q000378:metabolism
chemical_list: D000418:Albumins; D000596:Amino Acids; D003470:Culture Media; D007328:Insulin; D014508:Urea; D005934:Glucagon; D006854:Hydrocortisone