Lava lamp, a novel peripheral golgi protein, is required for Drosophila melanogaster cellularization.
Drosophila cellularization and animal cell cytokinesis rely on the coordinated functions of the microfilament and microtubule cytoskeletal systems. To identify new proteins involved in cellularization and cytokinesis, we have conducted a biochemical screen for microfilament/microtubule-associated proteins (MMAPs). 17 MMAPs were identified; seven have been previously implicated in cellularization and/or cytokinesis, including KLP3A, Anillin, Septins, and Dynamin. We now show that a novel MMAP, Lava Lamp (Lva), is also required for cellularization. Lva is a coiled-coil protein and, unlike other proteins previously implicated in cellularization or cytokinesis, it is Golgi associated. Our functional analysis shows that cellularization is dramatically inhibited upon injecting anti-Lva antibodies (IgG and Fab) into embryos. In addition, we show that brefeldin A, a potent inhibitor of membrane trafficking, also inhibits cellularization. Biochemical analysis demonstrates that Lva physically interacts with the MMAPs Spectrin and CLIP190. We suggest that Lva and Spectrin may form a Golgi-based scaffold that mediates the interaction of Golgi bodies with microtubules and facilitates Golgi-derived membrane secretion required for the formation of furrows during cellularization. Our results are consistent with the idea that animal cell cytokinesis depends on both actomyosin-based contraction and Golgi-derived membrane secretion.
Sisson
J C
JC
Department of Molecular, Cell and Developmental Biology, Sinsheimer Labs, University of California at Santa Cruz, Santa Cruz, California 95064, USA. sisson@darwin.ucsc.edu
Field
C
C
Ventura
R
R
Royou
A
A
Sullivan
W
W
D000199:Actins / Q000378:metabolism; D000818:Animals; D030801:Animals, Genetically Modified; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000196:embryology*; D004625:Embryo, Nonmammalian / Q000166:cytology* / Q000502:physiology*; D006056:Golgi Apparatus / Q000502:physiology*; D007425:Intracellular Membranes / Q000378:metabolism / Q000648:ultrastructure; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D011485:Protein Binding; D013049:Spectrin / Q000378:metabolism
D000199:Actins; D029721:Drosophila Proteins; C502291:Lva protein, Drosophila; D008869:Microtubule-Associated Proteins; D013049:Spectrin