Capillary electrophoresis-electrospray ion-trap mass spectrometry for the separation of chlorophenols.
Eighteen positional isomers of chlorophenols were separated by capillary electrophoresis (CE) and detected on-line by electrospray ionization ion-trap mass spectrometry (MS). Conditions for the coupling of CE to MS, e.g., the concentration of carrier electrolyte, the sheath liquid composition and the sheath gas flow-rate were optimized. Diethylmalonic acid (5 mM) at pH 7.25 and isopropanol-250 mM dimethylamine (80:20) as sheath liquid were used. The activation parameters for ion-trap mass spectrometric analysis of chlorophenols were optimized. The mass spectra, obtained for all the analytes, revealed that the [M-H]- ion was the base peak for all chlorophenols. Moreover, conditions for CE-MS-MS detection were established and [M-H-HCl]- ions were detected.
Jáuregui
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O
Departament de Química Analítica, Facultat de Química, Universitat de Barcelona, Spain.
Moyano
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E
Galceran
M T
MT
D002733:Chlorophenols / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods*; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D002733:Chlorophenols