title: mig-14 is a horizontally acquired, host-induced gene required for salmonella enterica lethal infection in the murine model of typhoid fever.
abstract: We have characterized a host-induced virulence gene, mig-14, that is required for fatal infection in the mouse model of enteric fever. mig-14 is present in all Salmonella enterica subspecies I serovars and maps to a region of the chromosome that appears to have been acquired by horizontal transmission. A mig-14 mutant replicated in host tissues early after infection but was later cleared from the spleens and livers of infected animals. Bacterial clearance by the host occurred concomitantly with an increase in gamma interferon levels and recruitment of macrophages, but few neutrophils, to the infection foci. We hypothesize that the mig-14 gene product may repress immune system functions by interfering with normal cytokine expression in response to bacterial infections.
authors.affiliation: Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford University, Stanford, California 94305, USA. valdivia@uclink4.berkeley.edu
authors.forename: R H
authors.initials: RH
authors.lastname: Valdivia
authors.forename: D M
authors.initials: DM
authors.lastname: Cirillo
authors.forename: A K
authors.initials: AK
authors.lastname: Lee
authors.forename: D M
authors.initials: DM
authors.lastname: Bouley
authors.forename: S
authors.initials: S
authors.lastname: Falkow
mesh_terms: D000818:Animals; D001483:Base Sequence; D016207:Cytokines / Q000096:biosynthesis; D004195:Disease Models, Animal; D005260:Female; D005798:Genes, Bacterial; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009569:Nitric Oxide / Q000502:physiology; D016366:Open Reading Frames; D019779:Salmonella enterica / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D014435:Typhoid Fever / Q000209:etiology* / Q000473:pathology; D014774:Virulence
chemical_list: D016207:Cytokines; D009569:Nitric Oxide