[Dynamic study of anti-HIV antibody avidity after cellular immunity restoration under antiretroviral treatment].
We have studied the evolution of the avidity of anti-HIV antibodies, in 14 infected patients with Aids, including 11 patients with severe immunodeficiency at Aids stage and under active antiretroviral therapy (HAART), and 3 non-treated patients with moderate immunodeficiency. These patients have been followed up to 4 years, every 4 months the first year and every 6 months the three others, with HIV1 RNA viral load, CD4 and CD8 cells dosages and anti-HIV avidity measurements (Axsym HIV-1/2), using 1 M guanidine denaturation. A rapid decrease of the viral load was observed under Haart, inducing immune restoration with CD4 and CD8 cells increases (10 and 2-fold respectively). The decrease of anti-HIV avidity (- 20%) has been observed after 5 to 8 months under Haart, with a return to baseline value (84%). The quick restoration of CD4 cells with a persistence of viral antigens at the beginning of treatment has facilitated the selection of novel naive B lymphocytes producing low-affinity antibodies, measured by the decrease of global anti-HIV avidity. The reduction or even clearance of viral antigens under Haart could secondarily induce the selection of B lymphocytes with higher antibody affinity and therefore higher anti-HIV avidity. Thus, this avidity measurement could be used to assess the functional activity of CD4 cells restoration in HIV infected patients under Haart.
Riou
M
M
Laboratoire de bactériologie-virologie, CHU, 4, rue Larrey, 49033 Angers cedex.
Renier
G
G
Mattman
S
S
Fialaire
P
P
Loison
J
J
Chennebault
J M
JM
Payan
C
C
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000628:therapy*; D000915:Antibody Affinity; D023241:Antiretroviral Therapy, Highly Active; D016516:CD4-CD8 Ratio; D005500:Follow-Up Studies; D015483:HIV Antibodies / Q000276:immunology*; D015497:HIV-1 / Q000235:genetics / Q000276:immunology; D006801:Humans; D012367:RNA, Viral / Q000032:analysis; D012189:Retrospective Studies; D013997:Time Factors; D019562:Viral Load
D015483:HIV Antibodies; D012367:RNA, Viral