title: Influence of additional acid etch treatment on resin cement dentin infiltration.
abstract: OBJECTIVE
  The purpose of this study was to compare the penetration of a resin cement into dentin surfaces pretreated with self-etching primer with or without conventional acid etch.
  
  
  METHODS
  Dentin surfaces of 8 unerupted human third molars were treated with self-etch primer (Panavia 21; Kuraray Co, Ltd, Osaka, Japan) with or without conventional acid etch treatment. A resin cement (Panavia 21) was applied according to manufacturer's instructions. Dentin/resin cement interface sections from each tooth were examined with light microscopy and scanning electron microscopy. In the light microscopy sections, exposed protein at the dentin/cement interface was stained a distinct red with Goldner's trichrome.
  
  
  RESULTS
  The resin cement did not penetrate the depth of the zone of demineralized dentin when the self-etch primer was used in combination with conventional acid etch treatment. Inadequate resin cement penetration leaves a substantial area of exposed protein at the dentin/cement interface. In contrast, there was complete resin cement diffusion throughout the demineralized dentin when the self-etch primer was used without acid etching.
  
  
  CONCLUSIONS
  Combining conventional acid etch treatment with a self-etching primer/resin cement system resulted in incomplete resin cement penetration and exposed protein at the dentin/cement interface.
authors.affiliation: Department of Oral Biology, UMKC School of Dentistry, 650 East 25th Street, Kansas City, MO 64108, USA. WalkerMP@umkc.edu
authors.forename: M P
authors.initials: MP
authors.lastname: Walker
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: Swafford
authors.forename: A
authors.initials: A
authors.lastname: Evans
authors.forename: P
authors.initials: P
authors.lastname: Spencer
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods*; D001391:Azo Compounds; D003094:Collagen / Q000648:ultrastructure; D004396:Coloring Agents; D003804:Dentin / Q000648:ultrastructure*; D004058:Diffusion; D004801:Eosine Yellowish-(YS); D006801:Humans; D008739:Methyl Green; D008855:Microscopy, Electron, Scanning; D008964:Molar, Third; D010710:Phosphates / Q000737:chemistry; D019279:Resin Cements / Q000737:chemistry*; D013499:Surface Properties; D014097:Tooth, Unerupted
chemical_list: D001391:Azo Compounds; D004396:Coloring Agents; C093719:Panavia TPN-S; D010710:Phosphates; D019279:Resin Cements; C017768:trichrome stain; D008739:Methyl Green; D003094:Collagen; D004801:Eosine Yellowish-(YS)