Quantification of Staphylococcus aureus cell surface adhesins using flow cytometry.
The initiation of many infectious diseases involves specific adhesion of bacteria to host tissue proteins and carbohydrates. Staphylococcus aureus is known to bind specifically to several proteins in the extracellular matrix (ECM). We report the quantification of the collagen and fibronectin adhesin densities on the staphylococcal surface using flow cytometry. Our results are in agreement with previous reports on the transcription of the respective genes and demonstrate different patterns of temporal expression for the two adhesins in the strains studied. We demonstrate a convenient technique for quantification of bacterial adhesins that can be used in studies aimed at characterization of bacterial adhesion to ECM components and understanding expression of adhesins during the course of an infection.
Mohamed
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Department of Chemical and Biochemical Engineering, University of Maryland, Baltimore County, Baltimore, MD 21250, USA.
Visai
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L
Speziale
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Ross
J M
JM
D018829:Adhesins, Bacterial / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology; D002352:Carrier Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology; D005434:Flow Cytometry; D015964:Gene Expression Regulation, Bacterial; D016023:Integrins / Q000032:analysis / Q000235:genetics / Q000502:physiology; D038981:Receptors, Collagen; D013211:Staphylococcus aureus / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D013997:Time Factors
D018829:Adhesins, Bacterial; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D002352:Carrier Proteins; D016023:Integrins; D038981:Receptors, Collagen; C078635:fibronectin-binding proteins, bacterial