Genetically synthesized antibody-binding protein self-assembled on hydrophobic matrix.
A unique antibody-binding protein, (E12B2)n, was genetically synthesized, which was characterized by a hydrophobic peptide, E12, at one terminus and an antibody-binding peptide, B2, at the other. It was clarified by atomic force microscopy (AFM) imaging that this protein was efficiently self-assembled on a hydrophobic solid surface. (E12B2)n self-assembled on a microplate exhibited an excellent performance of antibody-binding affinity. The proposed design of antibody-binding protein seems promising in immobilizing antibody molecules on hydrophobic solid surfaces.
Sugihara
T
T
Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama 226-8501, Japan.
Seong
G H
GH
Kobatake
E
E
Aizawa
M
M
D000906:Antibodies / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D018625:Microscopy, Atomic Force; D010957:Plasmids; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D000906:Antibodies; D011506:Proteins