Invertase production of common storage moulds in food and feed grains as a possibility for rapid detection of Aspergillus flavus group and Aspergillus fumigatus.
Invertase production of grain storage moulds was studied. Aspergillus spp. and Penicillium spp. were grown in a sucrose based liquid medium, at 37 degrees C. The A. flavus group (A. flavus, A. parasiticus, A. nomius, A. oryzae) and A. fumigatus showed a fast growth and intense invertase activity, while other Aspergillus spp. and Penicillium spp. grew slower and produced less invertase. The pattern of accumulated reducing sugar after 20 and 48 h of incubation was characteristic to the species studied. From inoculation studies the detection limit was calculated as: 1-10 conidia of A. flavus group and A. fumigatus, as compared to 10(3)-10(4) for the other species studied. The method may be recommended as a rapid test for the detection of A. flavus group and A. fumigatus in food and feed grains.
Mátrai
T
T
Research Institute for Animal Breeding and Nutrition, Gesztenyés, Hungary.
Mayer
S
S
Kókai
S
S
Salamon
I
I
D000821:Animal Feed / Q000382:microbiology*; D001231:Aspergillus flavus / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification; D001232:Aspergillus fumigatus / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification; D002523:Edible Grain / Q000382:microbiology*; D005516:Food Microbiology; D006026:Glycoside Hydrolases / Q000096:biosynthesis*; D013997:Time Factors; D043324:beta-Fructofuranosidase
D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase