Crystals of GlpE, a 12 kDa sulfurtransferase from escherichia coli, display 1.06 A resolution diffraction: a preliminary report.
The Escherichia coli sn-glycerol 3-phosphate regulon contains the glpE gene coding for a 12 kDa protein which displays a sequence and a thiosulfate:cyanide sulfurtransferase activity similar to those of rhodanese enzymes. The GlpE protein was overexpressed, purified to homogeneity and crystallized in the trigonal space group P3(1) (or P3(2)). The unit-cell parameters are a = b = 53.87, c = 30.52 A, gamma = 120 degrees. Evaluation of the crystal packing parameter establishes the presence of one molecule per asymmetric unit, with a solvent content of 42%. The GlpE crystals display very high resolution diffraction; a 1.06 A data set was collected using synchrotron radiation (lambda = 0.9102 A) with an overall completeness of 99.6%.
Bordo
D
D
National Institute for Cancer Research c/o Advanced Biotechnology Center, Largo R. Benzi 10, 16132 Genova, Italy. domenico@alcor.ge.infm.it
Larson
T J
TJ
Donahue
J L
JL
Spallarossa
A
A
Bolognesi
M
M
D018360:Crystallography, X-Ray; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins; D008970:Molecular Weight; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D013379:Substrate Specificity; D013466:Sulfurtransferases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification
D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C102897:GlpE protein, E coli; D011994:Recombinant Proteins; D013466:Sulfurtransferases