The stripping technique: in vitro absorption and penetration of five UV filters on excised fresh human skin.
This article gives the results of a study whose aim was to compare the compartmental distribution and absorption of 5 UV filters, in vitro, by fresh human skin, after exposure times of 30 min and 16 h. These UV filters from BASF (octyl methoxycinnamate, benzophenone 4, benzophenone 3, octyl triazone and octocrylene) were incorporated separately in a simple oil-in-water emulsion. The composition of the emulsions was designed in order to obtain a sun protection factor of 5. Therefore the UV filters were introduced into the emulsions at different concentrations. We show that the affinity for each skin level [stratum corneum (SC), viable epidermis, dermis and receptor fluid] is different according to the test substance used. Some substances accumulated in the SC, whereas others passed through the skin very quickly and were quantified in the receptor fluid. The stripping technique allowed us to see that more than 94% of the chemical compound in the SC was in the first eight tapes. The problem of individual values below the limit of detection was raised, a correlation between the two exposure times was found (y = 1.702x - 0.105; R = 0.94) and a classification of products according to their affinity for the SC was determined.
Potard
G
G
Research Unit in Dermopharmacology and Cosmetology, Faculty of Pharmacy, Châtenay-Malabry, France.
Laugel
C
C
Schaefer
H
H
Marty
J P
JP
D000328:Adult; D000368:Aged; D002851:Chromatography, High Pressure Liquid; D004058:Diffusion; D015194:Diffusion Chambers, Culture; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008875:Middle Aged; D012867:Skin / Q000737:chemistry; D012869:Skin Absorption / Q000502:physiology*; D013048:Specimen Handling / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet; D013473:Sunscreening Agents / Q000493:pharmacokinetics*; D014466:Ultraviolet Rays; D014870:Water Loss, Insensible / Q000502:physiology
D013473:Sunscreening Agents