The interferon-inducible Staf50 gene is downregulated during T cell costimulation by CD2 and CD28.
It is well established that interferons (IFN) exert potent regulatory effects on the immune system. We have recently isolated a new IFN-induced human cDNA coding for a member of the Ring finger B-box/B30.2 subfamily that localizes to the chromosome band 11p15. We have named it Staf50. We show in this report that Staf50 is expressed in resting T cells in the absence of exogenous IFN treatment and is strongly repressed during T cell activation by anti-CD28 and anti-CD2 monoclonal antibodies (mAb) at both messenger and protein levels. In addition, we show that several members of the Ring finger B-box/B30.2 subfamily, including the 52-kDa SSA/Ro autoantigen, localize to the same chromosome band, 11p15, and are upregulated by IFN. These data led us to define a family of IFN-induced genes clustered on chromosome 11p15 that may be involved in T cell regulatory processes.
Gongora
C
C
INSERM Unité U475, 34197 Montpellier cedex 5, France.
Tissot
C
C
Cerdan
C
C
Mechti
N
N
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D001324:Autoantigens / Q000235:genetics; D015152:Blotting, Northern; D015153:Blotting, Western; D018801:CD2 Antigens / Q000276:immunology*; D018106:CD28 Antigens / Q000276:immunology*; D002880:Chromosomes, Human, Pair 11 / Q000276:immunology; D005786:Gene Expression Regulation; D006801:Humans; D007372:Interferons / Q000494:pharmacology*; D008213:Lymphocyte Activation; D015778:Minor Histocompatibility Antigens; D008969:Molecular Sequence Data; D020733:RNA, Small Cytoplasmic; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000235:genetics; D016415:Sequence Alignment; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D000070584:Tripartite Motif Proteins; D000099207:SS-A Antigen
D000911:Antibodies, Monoclonal; D001324:Autoantigens; D018801:CD2 Antigens; D018106:CD28 Antigens; D007372:Interferons; D015778:Minor Histocompatibility Antigens; D020733:RNA, Small Cytoplasmic; D012097:Repressor Proteins; D012261:Ribonucleoproteins; D014157:Transcription Factors; D000070584:Tripartite Motif Proteins; D000099207:SS-A Antigen; C116006:RO60 protein, human; C094314:TRIM22 protein, human