title: Short-term exposure to membrane-active antibiotics inhibits Cryptosporidium parvum infection in cell culture.
abstract: A cell culture system and double fluorogenic staining were used to study the susceptibility of Cryptosporidium parvum to membrane-active antibiotics. Buforin II and magainin II exerted a cytotoxic effect on sporozoites but did not consistently affect oocyst viability. Lasalocid and nigericin demonstrated less activity against sporozoites but reduced the infectivity of oocysts.
authors.affiliation: Institute of Infectious Diseases and Public Health, University of Ancona, Ancona, Italy. cmalinf@popsci.unian.it
authors.forename: A
authors.initials: A
authors.lastname: Giacometti
authors.forename: O
authors.initials: O
authors.lastname: Cirioni
authors.forename: M S
authors.initials: MS
authors.lastname: Del Prete
authors.forename: F
authors.initials: F
authors.lastname: Barchiesi
authors.forename: G
authors.initials: G
authors.lastname: Scalise
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects; D016785:Cryptosporidium parvum / Q000166:cytology / Q000187:drug effects*; D004353:Drug Evaluation, Preclinical; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D006801:Humans; D054806:Magainins; D011506:Proteins / Q000494:pharmacology*; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000469:parasitology; D029867:Xenopus Proteins
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D005456:Fluorescent Dyes; D054806:Magainins; D011506:Proteins; D029867:Xenopus Proteins; C097849:buforin II; C053301:magainin 2 peptide, Xenopus