The predictive value of five different urinary LH kits in detecting the LH surge in regularly menstruating women.
OBJECTIVE
To compare the predictive value of five different urinary LH kits at detecting the LH surge in regularly menstruating, reproductive-age women.


METHODS
Single center, prospective study.


METHODS
University of Tennessee, Obstetrics and Gynecology department.


METHODS
Eleven regularly menstruating women collected urine daily from cycle days 10 through 18. Urinary LH was quantitated by radioimmunoassay. Transvaginal sonography was performed to document ovulation. Three different lots of Clear Plan Easy, OvuKit, OvuQuick, Sure Step, and EZ LH were evaluated.


METHODS
Correlation of urinary LH test kit results with urine LH value determined by RIA.


RESULTS
Peak urinary LH values by RIA ranged from 13.5 mIU/mL to 73.0 mIU/mL. The lowest level detected as positive by LH kits ranged from 25.5 mIU/mL to 48.7 mIU/mL. Lot-to-lot variations were rare. Follicular collapse occurred within 24 hours of the urinary LH peak in 8 of 10 (80%) and by 48 hours in the remaining 2 subjects.


CONCLUSIONS
The percentage of LH surges detected by urinary LH kits ranged from 50% to 100%. The lowest LH value detected as positive varied almost twofold between different kits. Manufacturers should indicate the detection limit of their kits in mIU/mL.
Ghazeeri
G S
GS
Department of Obstetrics and Gynecology, University of Tennessee, Memphis, 38163-2116, USA.
Vongprachanh
P
P
Kutteh
W H
WH
D000328:Adult; D005260:Female; D006801:Humans; D007986:Luteinizing Hormone / Q000652:urine*; D008598:Menstruation / Q000652:urine*; D010060:Ovulation; D011237:Predictive Value of Tests; D011446:Prospective Studies; D011863:Radioimmunoassay; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
D011933:Reagent Kits, Diagnostic; D007986:Luteinizing Hormone