title: Quantitative diversity of 67 kda protective antigen among serovar 2 strains of Erysipelothrix rhusiopathiae and its implication in protective immune response.
abstract: Mouse monoclonal antibodies (MAbs), raised against the NaOH-extracted antigen of Erysipelothrix rhusiopathiae strain Kyoto (serovar 2), recognized two different epitopes on a single protein of molecular weight 67 kDa. The MAbs were classified as protective or non-protective against strain Fujisawa (serovar 1). In immunoblotting analysis using the MAbs, fifteen wild strains were shown to contain different amounts of 67 kDa protective antigen. Each formalin-killed whole cell vaccine (bacterin) prepared from the fifteen wild strains conferred different levels of protection against strain Fujisawa in mice. Bacterins prepared from wild strains with larger amounts of 67 kDa protective antigen tended to give high levels of antigen-specific antibody and better protection to mice. These results indicate that the amount of 67 kDa protective antigen which influences the induction of protective immune responses may vary substantially among the strains of E. rhusiopathiae (serovar 2).
authors.affiliation: Division of Veterinary Microbiology, Kyoto Biken Laboratories, Uji, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kitajima
authors.forename: E
authors.initials: E
authors.lastname: Oishi
authors.forename: K
authors.initials: K
authors.lastname: Amimoto
authors.forename: U
authors.initials: U
authors.lastname: Satoshi
authors.forename: H
authors.initials: H
authors.lastname: Nakamura
authors.forename: K
authors.initials: K
authors.lastname: Oda
authors.forename: S
authors.initials: S
authors.lastname: Katayama
authors.forename: A
authors.initials: A
authors.lastname: Izumida
authors.forename: Y
authors.initials: Y
authors.lastname: Shimizu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000940:Antigenic Variation / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D000939:Epitopes / Q000276:immunology; D004888:Erysipelothrix / Q000145:classification / Q000276:immunology*; D004889:Erysipelothrix Infections / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control; D005260:Female; D007116:Immunization, Passive / Q000662:veterinary; D015151:Immunoblotting / Q000662:veterinary; D007132:Immunoglobulin Isotypes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight
chemical_list: D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D000939:Epitopes; D007132:Immunoglobulin Isotypes