Plasminogen, alpha(2)-antiplasmin and complexes of plasmin-alpha(2)-antiplasmin (PAP) in amniotic fluid and blood plasma of parturient women.
The aim of the present study was to determine whether the following components of the fibrinolytic system are present in amniotic fluid: plasminogen, plasmin and alpha(2)-antiplasmin, and plasmin-alpha(2)-antiplasmin (PAP) complexes. The study group consisted of 30 healthy women giving birth at term; 10 nonpregnant women constituted a control group. Plasminogen and alpha(2)-antiplasmin were studied by using an amidolytic method, and PAP by an ELISA method. The levels of plasminogen and alpha(2)-antiplasmin in amniotic fluid are low, 1.57+/-0.82 and 6.80+/-3.09%, respectively, of the values in blood plasma. The PAP concentration in amniotic fluid (70.61+/-14.28 microg/l) was also lower than in blood plasma (247.19+/-111.39 microg/l). We concluded that in amniotic fluid plasminogen and alpha(2)-antiplasmin are present, but at a concentration lower than in plasma. The presence of PAP in amniotic fluid is an evidence of two processes in the amniotic cavity: (i) plasmin generation, and (ii) plasmin inactivation by alpha(2)-antiplasmin.
Uszynski
M
M
Department of Medical Propedeutics, Ludwik Rydygier Medical University ul. M. Curie-Sklodowskiej 9, 85 067 Bydgoszcz, Poland.
Klyszejko
A
A
Zekanowska
E
E
D000328:Adult; D000653:Amniotic Fluid / Q000737:chemistry*; D005260:Female; D005341:Fibrinolysin / Q000032:analysis*; D006801:Humans; D010958:Plasminogen / Q000032:analysis*; D049590:Postpartum Period / Q000378:metabolism*; D011247:Pregnancy; D000979:alpha-2-Antiplasmin / Q000032:analysis*; D000511:alpha-Macroglobulins / Q000032:analysis*
D000979:alpha-2-Antiplasmin; D000511:alpha-Macroglobulins; C024668:plasmin-alpha(2)-macroglobulin complex; D010958:Plasminogen; D005341:Fibrinolysin