Receptor-mediated cell modulator delivery to hepatocyte using nanoparticles coated with carbohydrate-carrying polymers.
Cell modulators such as colchicine (CO), cytochalasin B (CY) and taxol (TX) loaded nanoparticles coated with carbohydrate-carrying polymers, as hepatocyte-specific targeting material using galactose ligands as recognition signals to asialoglycoprotein receptors were prepared by the diafiltration method. Effects of cell modulators from their loaded nanoparticles on morphology of hepatocytes were studied. Receptor-mediated endocytosis of the nanoparticles were examined by fluorescence and confocal laser microscopy. It was found that the shapes of most hepatocytes were changed for the CY-loaded, TX-loaded, or CO-loaded nanoparticles whereas their shapes were not changed in comparison with control when CY, TX, or CO were mixed with the nanoparticles. From the fluorescence and confocal laser microscopic studies, it is suggested that the nanoparticles coated with sugar-carrying polymers were internalized by the hepatocytes through the receptor-mediated mechanism.
Cho
C S
CS
School of Agricultural Biotechnology, Seoul National University, Suwon, South Korea. chocs@plaza.snu.ac.kr
Kobayashi
A
A
Takei
R
R
Ishihara
T
T
Maruyama
A
A
Akaike
T
T
D000818:Animals; D003078:Colchicine / Q000008:administration & dosage* / Q000494:pharmacology; D003571:Cytochalasin B / Q000008:administration & dosage* / Q000494:pharmacology; D004337:Drug Carriers; D004705:Endocytosis; D005260:Female; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects*; D008099:Liver / Q000166:cytology; D051379:Mice; D017239:Paclitaxel / Q000008:administration & dosage*; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000502:physiology*
D004337:Drug Carriers; D011956:Receptors, Cell Surface; C003172:poly-gamma-benzyl-L-glutamate; D011099:Polyglutamic Acid; D003571:Cytochalasin B; D017239:Paclitaxel; D003078:Colchicine