title: Neuropathogenesis of chimeric simian human immunodeficiency virus infection in rhesus macaques.
abstract: Comparative studies were performed to determine the neuropathogenesis of infection in macaques with simian human immunodeficiency virus (SHIV)89.6P and SHIV(KU). Both viruses utilize the CD4 receptor and CXCR4 co-receptor. However, in addition, SHIV89.6P uses the CCR5 co-receptor. Both agents are dual tropic for CD4+ T cells and blood-derived macrophages of rhesus macaques. Following inoculation into macaques, both caused rapid elimination of CD4+ T cells but they varied greatly in mechanisms of neuropathogenesis. Two animals infected with SHIV89.6P developed typical lentiviral encephalitis in which multinucleated giant cell formation, nodular accumulations of microglial cells, activated macrophages and astrocytes, and perivascular accumulations of mononuclear cells were present in the brain. Many of the macrophages in these lesions contained viral RNA. Three macaques infected with SHIV(KU) and killed on days 6, 11 and 18, respectively, developed a slowly progressive infection in the CNS but macrophages were not productively infected and there were no pathological changes in the brain. Two other animals infected with this virus and killed several months later showed minimal infection in the brain even though one of the two developed encephalitis of unknown etiology. The basic difference in the mechanisms of neuropathogenesis by the two viruses may be related to co-receptor usage. SHIV89.6P, in utilizing the CCR5 co-receptor, caused neuropathogenic effects that are similar to other neurovirulent primate lentiviruses.
authors.affiliation: Department of Microbiology, Immunology, and Molecular Genetics, University of Kansas Medical Center, Kansas City 66160, USA.
authors.forename: S
authors.initials: S
authors.lastname: Buch
authors.forename: D
authors.initials: D
authors.lastname: Pinson
authors.forename: Y
authors.initials: Y
authors.lastname: Hou
authors.forename: I
authors.initials: I
authors.lastname: Adany
authors.forename: Z
authors.initials: Z
authors.lastname: Li
authors.forename: S
authors.initials: S
authors.lastname: Mukherjee
authors.forename: F
authors.initials: F
authors.lastname: Jia
authors.forename: G
authors.initials: G
authors.lastname: Mackay
authors.forename: P
authors.initials: P
authors.lastname: Silverstein
authors.forename: A
authors.initials: A
authors.lastname: Kumar
authors.forename: O
authors.initials: O
authors.lastname: Narayan
mesh_terms: D015526:AIDS Dementia Complex / Q000276:immunology / Q000473:pathology / Q000503:physiopathology*; D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000503:physiopathology*; D000818:Animals; D001921:Brain / Q000276:immunology / Q000473:pathology / Q000821:virology*; D015704:CD4 Antigens / Q000502:physiology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000821:virology; D002460:Cell Line; D002478:Cells, Cultured; D002678:Chimera; D018450:Disease Progression; D015754:Genes, nef; D006678:HIV / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D006801:Humans; D008253:Macaca mulatta; D008264:Macrophages / Q000276:immunology / Q000821:virology; D012367:RNA, Viral / Q000302:isolation & purification; D019718:Receptors, CXCR4 / Q000502:physiology; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000503:physiopathology*; D015302:Simian Immunodeficiency Virus / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D013601:T-Lymphocytes
chemical_list: D015704:CD4 Antigens; D012367:RNA, Viral; D019718:Receptors, CXCR4