Molecular evolutionary analysis of the complete nucleotide sequence of hepatitis B virus (HBV) in a case of HBV infection acquired through a needlestick accident.
To elucidate needlestick transmission of hepatitis B virus (HBV), strains isolated from 1 physician who acquired HBV infection through a needlestick accident and 3 patients with chronic hepatitis B (donor patients A, B, and C) were tested using molecular evolutionary analysis based on full-length HBV genomic sequences. Nucleotide sequences of these isolates were aligned with 55 previously reported full-length genomic sequences. Genetic distances were estimated using the 6-parameter method, and phylogenetic trees were constructed using the neighbor-joining method. Strains isolated from patient A and the recipient pair were clustered within a closer range of evolutionary distances than were strains recovered from the recipient pair and patients B and C. Furthermore, strains from patient A and the recipient were also clustered on the S gene sequences of HBV. These results demonstrated that patient A alone was the source of direct transmission to the recipient. This approach can be used to investigate the transmission route of HBV.
Sugauchi
F
F
Second Department of Internal Medicine/Blood Transfusion, Nagoya City University Medical School, Nagoya, Japan.
Mizokami
M
M
Orito
E
E
Ohno
T
T
Hayashi
K
K
Kato
T
T
Tanaka
Y
Y
Kato
H
H
Ueda
R
R
D000328:Adult; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D019143:Evolution, Molecular; D005260:Female; D006509:Hepatitis B / Q000097:blood / Q000635:transmission / Q000821:virology*; D006514:Hepatitis B Surface Antigens / Q000097:blood; D006515:Hepatitis B virus / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016602:Needlestick Injuries / Q000821:virology*; D009784:Occupational Diseases / Q000821:virology; D010802:Phylogeny; D017422:Sequence Analysis, DNA
D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens