Red light kills bacteria via photodynamic action.
With the increase in the number of antibiotic resistant strains of microorganism, the search for alternative treatments of microbial infections becomes all the more important. We report a novel method for bacterial inactivation based on the optical excitation of the naturally occurring (endogenous) photosensitzing porphyrins by red light. In particular, the pathogenic Gram-positive porphyrin producing ATCC strains Propionibacterium acnes, Actinomyces odontolyticus and Porphyromonas gingivalis were investigated. Sensitive autofluorescence spectroscopy revealed that these bacteria naturally synthezise the fluorescent photosensitizer protoporphyrin IX. In addition, bacterial plaque samples of periodontitis patients were studied. Non-labeled fluorescent bacterial colonies were exposed to red light at 632.8 nm, 100 mW/cm2 light intensity and 360 J/cm2 energy density using a helium-neon laser. The survival rate after a single phototreatment with red light was found to be 0.58 +/- 0.09 in the case of Propionibacterium acnes, 0.30 +/- 0.04 in Actinomyces odontolyticus and 0.59 +/- 0.10 in Porphyrormonas gingivalis compared to non-exposed bacteria suspensions. No photoeffect was found for the bacterium Streptococcus mutans which exhibited no detectable porphyrin autofluorescence. Red-light exposed plaque samples of patients showed significant reduction of colony forming units by 50% as well as a pronounced photoeffect on the pigmented species Prevotella intermedia. Taken together, these results suggest the treatment with red light can be potentially employed as an therapeutic method to inactivate certain pathogenic strains of porphyrin producing bacteria without the use of external photosensitizers.
König
K
K
Institute of Anatomy II, Friedrich Schiller University Jena, Germany. kkoe@mti-n.uni-jena.de
Teschke
M
M
Sigusch
B
B
Glockmann
E
E
Eick
S
S
Pfister
W
W
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002455:Cell Division / Q000528:radiation effects; D015169:Colony Count, Microbial; D003116:Color; D003731:Dental Caries / Q000382:microbiology / Q000628:therapy; D004352:Drug Resistance, Microbial; D006094:Gram-Positive Bacteria / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects*; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology / Q000628:therapy; D006801:Humans; D053685:Laser Therapy; D008027:Light; D010518:Periodontitis / Q000382:microbiology / Q000628:therapy; D017319:Photosensitizing Agents / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects; D011166:Porphyrins / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects*; D013050:Spectrometry, Fluorescence; D013357:Subgingival Curettage; D014640:Vancomycin / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D017319:Photosensitizing Agents; D011166:Porphyrins; D014640:Vancomycin