title: Serum markers of lamellar basement membrane degradation and lamellar histopathological changes in horses affected with laminitis.
abstract: In order better to evaluate the extent to which degradation of the lamellar basement membrane (LBM) by matrix metalloproteinases (MMP) occurs in equine laminitis, we determined the concentration of type IV collagen and laminin in normal and laminitic horses, using specific immunoassays. Blood samples were obtained from both the jugular and the cephalic veins of horses (n = 10) before and after the induction of acute alimentary laminitis by carbohydrate overload. Jugular and cephalic venous blood samples were also obtained from horses affected with naturally occurring laminitis (n = 16) and nonlaminitic controls (n = 8). The serum collagen IV concentration was not changed following the induction of laminitis in the experimental group. Serum collagen IV concentration was increased in jugular venous blood obtained from cases of naturally occurring laminitis (mean +/- s.e. 218.04 +/- 18.59 ng/ml) compared with nonlaminitic controls (157.50 +/- 10.93 ng/ml) (P<0.05). Serum collagen IV concentration was also increased in jugular venous blood obtained from severely laminitic horses (219.50 +/- 18.18 ng/ml) compared with nonlaminitic controls (157.50 +/- 10.93 ng/ml) (P<0.05). A difference in serum concentration of collagen IV was not identified based on chronicity of naturally occurring laminitis. Serum laminin concentration did not differ between laminitic and nonlaminitic horses. Differences in serum laminin concentration were not identified based on sampling location (jugular or cephalic vein), severity of laminitic pain, or chronicity of spontaneous laminitis. In conclusion, the circulating concentration of collagen IV was increased in horses affected with naturally occurring laminitis. The potential role for serum collagen IV assay for characterisation of equine laminitis warrants further investigation.
authors.affiliation: Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, Columbia 65211, USA.
authors.forename: P J
authors.initials: PJ
authors.lastname: Johnson
authors.forename: J M
authors.initials: JM
authors.lastname: Kreeger
authors.forename: M
authors.initials: M
authors.lastname: Keeler
authors.forename: V K
authors.initials: VK
authors.lastname: Ganjam
authors.forename: N T
authors.initials: NT
authors.lastname: Messer
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000473:pathology; D015415:Biomarkers; D003094:Collagen / Q000097:blood*; D005260:Female; D005534:Foot Diseases / Q000473:pathology / Q000662:veterinary*; D006724:Hoof and Claw; D006734:Horse Diseases / Q000097:blood / Q000473:pathology*; D006736:Horses; D007118:Immunoassay / Q000662:veterinary; D007794:Lameness, Animal / Q000473:pathology*; D007797:Laminin / Q000097:blood*; D008297:Male
chemical_list: D015415:Biomarkers; D007797:Laminin; D003094:Collagen