title: First isolation of a rabies-related virus from a Daubenton's bat in the United Kingdom.
abstract: On May 30, 1996, a sick Daubenton's bat (Myotis daubentonii) was recovered from the cellar of a public house in Newhaven, East Sussex. Its condition deteriorated rapidly, and it was euthanased and examined. Positive results, establishing the presence of a rabies or rabies-related virus in its brain, were obtained from the fluorescent antibody test, the rabies tissue culture isolation test, and a hemi-nested reverse-transcription PCR. The complete sequence of the nucleoprotein gene was determined and a phylogenetic analysis, based on the 470 nucleotide bases of the amino terminus of the nucleoprotein, established the genotype of the virus as European bat lyssavirus 2. Bat rabies had not previously been recorded in the UK but does occur in mainland Europe. A study of the back-trajectories of the wind on May 29 and 30, established that the infected bat possibly came from near the Franco-Swiss border.
authors.affiliation: The Wellcome Trust, London.
authors.forename: J E
authors.initials: JE
authors.lastname: Whitby
authors.forename: P R
authors.initials: PR
authors.lastname: Heaton
authors.forename: E M
authors.initials: EM
authors.lastname: Black
authors.forename: M
authors.initials: M
authors.lastname: Wooldridge
authors.forename: L M
authors.initials: LM
authors.lastname: McElhinney
authors.forename: P
authors.initials: P
authors.lastname: Johnstone
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000821:virology; D002685:Chiroptera / Q000821:virology*; D005455:Fluorescent Antibody Technique; D005838:Genotype; D008969:Molecular Sequence Data; D010802:Phylogeny; D011820:Rabies virus / Q000235:genetics* / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D006113:United Kingdom