Application of BARE-1 retrotransposon markers to the mapping of a major resistance gene for net blotch in barley.
Net blotch, which is caused by the fungus Pyrenophoral teres Drechs. f. teres Smedeg., presents a serious problem for barley production worldwide, and the identification and deployment of sources of resistance to it are key objectives for many breeders. Here, we report the identification of a major resistance gene, accounting for 65% of the response variation, in a cross between the resistant line C19819 and the susceptible cv. Rolfi. The resistance gene was mapped to chromosome 6H with the aid of two recently developed systems of retrotransposon-based molecular markers, REMAP and IRAP. A total of 239 BARE-1 and Sukkula retrotransposon markers were mapped in the cross, and the 30-cM segment containing the locus with significant resistance effect contained 26 of the markers. The type and local density of the markers should facilitate future map-based cloning of the resistance gene as well as manipulation of the resistance through backcross breeding.
Manninen
O
O
Institute of Biotechnology, University of Helsinki, Plant Genomics Laboratory, Viikki Biocenter, Finland.
Kalendar
R
R
Robinson
J
J
Schulman
A H
AH
D000483:Alleles; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D017343:Genes, Plant / Q000235:genetics*; D008040:Genetic Linkage; D005819:Genetic Markers; D001467:Hordeum / Q000235:genetics*; D007113:Immunity, Innate / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics; D008957:Models, Genetic; D010940:Plant Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D019655:Quantitative Trait, Heritable; D018626:Retroelements
C082800:BARE-1 protein, Hordeum vulgare; D005819:Genetic Markers; D010940:Plant Proteins; D018626:Retroelements