Expression of TAFII70 RNA and protein during oogenesis and development of the amphibian Pleurodeles waltl.
TAFs are thought to play an essential role in eukaryotic RNA polymerase II transcription by mediating the expression of distinct subsets of genes. TAFII60/70 was studied in yeast, Drosophila and humans: in the present work, we analyzed the homologue PwTAFII70 in Pleurodeles. The gene is expressed in ovarian oocytes and throughout development, and the level of expression decreases in late embryos. The transcripts are localized in the animal hemisphere of the fertilized eggs and in the animal blastomeres of embryos at cleavage; later PwTAFII70 mRNA is expressed in the neural plate and folds. TAFII70 protein, which is present in fertilized eggs and throughout development, progressively shows a lower level of expression starting from the neurula stage.
Giani
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Dipartimento di Fisiologia e Biochimica, Laboratorio di Biologia cellulare e dello sviluppo, Università degli Studi di Pisa, Pisa, Italy.
Bucci
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Ragghianti
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Mancino
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D000595:Amino Acid Sequence; D000818:Animals; D001757:Blastomeres / Q000378:metabolism; D015152:Blotting, Northern; D015153:Blotting, Western; D003593:Cytoplasm / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000378:metabolism; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism*; D009866:Oogenesis; D010999:Pleurodeles / Q000235:genetics*; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D035381:TATA-Binding Protein Associated Factors; D013997:Time Factors; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics*
D018076:DNA, Complementary; D012333:RNA, Messenger; C084977:TAF6 protein, human; D035381:TATA-Binding Protein Associated Factors; D014157:Transcription Factors; D012313:RNA