The novel isoform of the progesterone receptor cDNA in the human testis and detection of its mRNA in the human uterine endometrium.
A novel isoform (termed isoform S) of the progesterone receptor (PR) cDNA (PR isoform S cDNA) which consists of a previously unidentified 5' sequence and exons 4-8 of the intracellular PR gene has been cloned from the human testicular cDNA library. The 5' sequence of the message was confirmed to be derived from a novel exon (termed exon S) by genomic cloning. The expression level of the PR isoform S mRNA was higher in the spermatozoon than in the uterine endometrium with a lower expression level of the PR isoforms B and A mRNAs in the spermatozoon than in the endometrium. These results implied that the PR isoform S which was possibly translated from the PR isoform S mRNA in the spermatozoon might be related to the cell surface membrane PR. Moreover, the PR isoform S in the uterine endometrium might play some physiological and/or pathogenic roles.
Hirata
S
S
Department of Obstetrics and Gynecology, Yamanashi Medical University, Nakakoma, Yamanashi, Japan. shirata@res.yamanashi-med.ac.jp
Shoda
T
T
Kato
J
J
Hoshi
K
K
D001483:Base Sequence; D015139:Blotting, Southern; D018076:DNA, Complementary / Q000737:chemistry*; D004717:Endometrium / Q000737:chemistry*; D005260:Female; D006801:Humans; D007536:Isomerism; D008297:Male; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis*; D011980:Receptors, Progesterone / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013737:Testis / Q000737:chemistry*
D018076:DNA, Complementary; D012333:RNA, Messenger; D011980:Receptors, Progesterone