title: Transcript analysis of 203 novel genes from Saccharomyces cerevisiae in hap1 and rox1 mutant backgrounds.
abstract: Hap1 and Rox1 are transcriptional regulators that bind regulatory sites in the promoters of oxygen-regulated genes in Saccharomyces cerevisiae. Hap1 is a heme-responsive activator of genes induced in aerobic conditions and Rox1 is a repressor of hypoxic genes in aerobic conditions. We have studied transcriptional regulation of a pool of 203 open reading frames (ORFs) from chromosomes IV, VII, and XIV in wild-type, hap1, and rox1 mutant genetic backgrounds in an attempt to extend the family of oxygen and heme regulated genes. Only three ORFs are significantly repressed by Rox1 but they cannot be considered as typical hypoxic genes because they are not overexpressed during hypoxia.
authors.affiliation: Departamento de Biología Celular y Molecular, Universidad de La Coruña, Spain.
authors.forename: L J
authors.initials: LJ
authors.lastname: Lombardía
authors.forename: J L
authors.initials: JL
authors.lastname: Cadahía-Rodríguez
authors.forename: M A
authors.initials: MA
authors.lastname: Freire-Picos
authors.forename: M I
authors.initials: MI
authors.lastname: González-Siso
authors.forename: A M
authors.initials: AM
authors.lastname: Rodríguez-Torres
authors.forename: M E
authors.initials: ME
authors.lastname: Cerdán
mesh_terms: D019757:Carbon-Oxygen Lyases / Q000235:genetics*; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D004268:DNA-Binding Proteins / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods; D015966:Gene Expression Regulation, Fungal; D006418:Heme / Q000378:metabolism; D009154:Mutation; D016366:Open Reading Frames; D010100:Oxygen / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C083348:ROX1 protein, S cerevisiae; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D006418:Heme; D019757:Carbon-Oxygen Lyases; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D010100:Oxygen