title: ICAM-1 enhances MHC-peptide activation of CD8(+) T cells without an organized immunological synapse.
abstract: In addition to the TCR-ligand interaction, other receptor-ligand pairs, such as LFA-1 and ICAM-1, play a major role in the activation of T cells. Recent studies of T cell activation suggest a coordinated movement of LFA-1 and ICAM-1 in forming a defined zone in the immunological synapse. It is unclear from these studies whether the organized molecular geometry of the immunological synapse is necessary for ICAM-1 enhancement of T cell activation. In this report, we demonstrate that ICAM-1 can enhance the activation of CD8(+) T cells by MHC-peptide in the absence of an organized immunologic synapse. Therefore, although the molecular organization of the immunologic synapse may amplify stimuli, it is not an absolute requirement for either CD8(+) T cell activation or the ICAM-1 enhancement of TCR activation.
authors.affiliation: Division of Monoclonal Ab, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA.
authors.forename: J S
authors.initials: JS
authors.lastname: Goldstein
authors.forename: T
authors.initials: T
authors.lastname: Chen
authors.forename: E
authors.initials: E
authors.lastname: Gubina
authors.forename: R W
authors.initials: RW
authors.lastname: Pastor
authors.forename: S
authors.initials: S
authors.lastname: Kozlowski
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000276:immunology; D008285:Major Histocompatibility Complex / Q000276:immunology*; D051379:Mice; D008822:Mice, Transgenic; D011971:Receptors, Immunologic / Q000276:immunology; D015398:Signal Transduction / Q000276:immunology
chemical_list: D016169:Lymphocyte Function-Associated Antigen-1; D011971:Receptors, Immunologic; D018799:Intercellular Adhesion Molecule-1