ICAM-1 enhances MHC-peptide activation of CD8(+) T cells without an organized immunological synapse.
In addition to the TCR-ligand interaction, other receptor-ligand pairs, such as LFA-1 and ICAM-1, play a major role in the activation of T cells. Recent studies of T cell activation suggest a coordinated movement of LFA-1 and ICAM-1 in forming a defined zone in the immunological synapse. It is unclear from these studies whether the organized molecular geometry of the immunological synapse is necessary for ICAM-1 enhancement of T cell activation. In this report, we demonstrate that ICAM-1 can enhance the activation of CD8(+) T cells by MHC-peptide in the absence of an organized immunologic synapse. Therefore, although the molecular organization of the immunologic synapse may amplify stimuli, it is not an absolute requirement for either CD8(+) T cell activation or the ICAM-1 enhancement of TCR activation.
Goldstein
J S
JS
Division of Monoclonal Ab, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA.
Chen
T
T
Gubina
E
E
Pastor
R W
RW
Kozlowski
S
S
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000276:immunology; D008285:Major Histocompatibility Complex / Q000276:immunology*; D051379:Mice; D008822:Mice, Transgenic; D011971:Receptors, Immunologic / Q000276:immunology; D015398:Signal Transduction / Q000276:immunology
D016169:Lymphocyte Function-Associated Antigen-1; D011971:Receptors, Immunologic; D018799:Intercellular Adhesion Molecule-1