title: Expression of 17beta-hydroxysteroid dehydrogenases in mesophilic and extremophilic yeast.
abstract: 17beta-hydroxysteroid dehydrogenases (17beta-HSDs) are enzymes responsible for reversible interconversions of biologically active 17-hydroxy and inactive 17-keto steroids. We have performed a survey of 17beta-HSD activity in yeast. Constitutive 17beta-HSD activity was found in three mesophilic yeast species: Candida tropicalis, Cryptococcus tsukubaensis, and Saccharomyces cerevisiae as well as in three extremophilic black yeast species: Hortaea werneckii, Trimmatostroma salinum, and Phaeotheca triangularis, indicating that 17beta-HSD activity is widely distributed among yeast. In extremophilic black yeast, NaCl modulated enzyme activity. Enzymes resembling 17beta-HSD from the filamentous fungus Cochliobolus lunatus were detected in Trimmatostroma salinum and Phaeotheca triangularis. Sequences with identity to the Saccharomyces cerevisiae YBR159w gene were not observed in other yeast species possessing a similar enzyme activity. The results suggest the existence of at least three different types of 17beta-HSD in yeast.
authors.affiliation: Institute of Biochemistry, Medical Faculty, Vrazov trg 2, 1000, Ljubljana, Slovenia.
authors.forename: T L
authors.initials: TL
authors.lastname: Rizner
authors.forename: J
authors.initials: J
authors.lastname: Adamski
authors.forename: M
authors.initials: M
authors.lastname: Zakelj-Mavric
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D012965:Sodium Chloride / Q000494:pharmacology; D015003:Yeasts / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics
chemical_list: D012965:Sodium Chloride; D015067:17-Hydroxysteroid Dehydrogenases