title: Dissolution profiles of principal ingredients in Kampo medicinal powders by high-performance liquid chromatography.
abstract: We describe here a method using HPLC for the simultaneous determination of albiflorin, paeoniflorin, glycyrrhizin and six flavanone glycosides (liquiritin, liquiritin apioside, naringin, neohesperidin, hesperidin and narirutin) in the Kampo medicines, Shigyaku-san and Haino-san. All nine components were separated in less than 40 min by linear gradient elution using a mobile phase containing aqueous phosphoric acid and acetonitrile. The dissolution of these components from powders of Shigyaku-san in aqueous solution at pH 1.80, 4.08 and 6.89 was examined. All of the components except glycyrrhizin were dissolved entirely within 5 min regardless of pH. Dissolution of glycyrrhizin was dependent on the pH of the aqueous solution, and increased with increasing pH.
authors.affiliation: Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Hiroshima, Japan. okamura@fupharm.fukuyama-u.ac.jp
authors.forename: N
authors.initials: N
authors.lastname: Okamura
authors.forename: T
authors.initials: T
authors.lastname: Maki
authors.forename: S
authors.initials: S
authors.lastname: Ishida
authors.forename: C
authors.initials: C
authors.lastname: Uraguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Onishi
authors.forename: E
authors.initials: E
authors.lastname: Sadasue
authors.forename: Y
authors.initials: Y
authors.lastname: Tsuruta
authors.forename: A
authors.initials: A
authors.lastname: Yagi
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D020835:Medicine, Kampo; D011208:Powders; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet
chemical_list: D004365:Drugs, Chinese Herbal; D011208:Powders