Quantitation of spermatogenesis by DNA flow cytometry from fine-needle aspiration cytology material.
DNA flow cytometry (FCM) was performed from fine-needle aspiration cytology (FNAC) of testis in 15 cases of male infertility to quantitate spermatogenesis. The results were correlated with FNAC findings. DNA FCM showed a ploidy relationship of haploid (1N) > diploid (2N) > tetraploid (4N) in cases of normal spermatogenesis. A ploidy relation of 2N > 1N > 4N was observed in cases of hypospermatogenesis or maturation arrest. In Sertoli cell-only cases, there were only 2N populations of cells. With the help of DNA FCM, a rapid and objective assessment of spermatogenesis is possible from FNAC of the testis.
Dey
P
P
Department of Cytology, Post Graduate Institute of Medical Education and Research, Chandigarh, India. pranab@ch1.dot.net.in
Mondal
A K
AK
Singh
S K
SK
Vohra
H
H
D001707:Biopsy, Needle; D004247:DNA / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007248:Infertility, Male / Q000235:genetics* / Q000473:pathology; D008297:Male; D009845:Oligospermia / Q000235:genetics / Q000473:pathology; D011003:Ploidies; D012708:Sertoli Cells / Q000473:pathology; D013091:Spermatogenesis / Q000235:genetics*; D013577:Syndrome; D013737:Testis / Q000473:pathology*
D004247:DNA