Molecular cloning and phylogenetic analysis of canine beta-casein.
A canine beta-casein cDNA was isolated from mammary tissue by polymerase chain reaction (PCR) using degenerate primers. It encodes 250 amino acids protein containing the conserved sequence motif of beta-casein. It showed the highest homology with snow-leopard (Uncia uncia (55-62% identity). It also showed 44-53% identity with human, 33-42%, identity with mouse, 29-37%, identity with rat, 43-53% identity with rabbit, 41-48% identity with pig, 44-51% identity with cattle and 44-50% identity with sheep. A 1.2-kb mRNA was detected in mammary tissue by Northern blot analysis. Phylogenetic analysis revealed that canine beta-casein formed a branch with lesser panda and snow leopard, which were grouped into carnivore.
Watanabe
M
M
Department of Veterinary Pathology, Faculty of Agriculture, Miyazaki University, Japan.
Sugano
S
S
Togashi
T
T
Imai
J
J
Uchida
K
K
Yamaguchi
R
R
Tateyama
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002020:Buffaloes; D002335:Carnivora; D002364:Caseins / Q000737:chemistry / Q000235:genetics*; D002417:Cattle; D003001:Cloning, Molecular; D004285:Dogs / Q000235:genetics*; D005260:Female; D006041:Goats; D006801:Humans; D008321:Mammary Glands, Animal / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D011817:Rabbits; D051381:Rats; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012756:Sheep; D013552:Swine
D002364:Caseins