The p53 status in juvenile chronic arthritis and rheumatoid arthritis.
The aim of this study was to investigate the p53 status in two autoimmune diseases; juvenile chronic arthritis (JCA) and rheumatoid arthritis (RA). In a PCR-sequencing analysis of exons 4-9 of the p53 gene, no mutation was identified, except for the case of an RA synovectomy sample with two mutations of intron 7. p53 gene polymorphisms for codons 36, 47, and 213 were not detected. Codon 72 polymorphism showed an indication of an increased occurrence of the Pro/Pro allelotype in JCA. Expression of P53 protein was comparable for JCA and RA synovectomy samples. For all RA samples P53 protein was detectable, whereas one sample of a JCA patient failed to express P53 protein.
Taubert
H
H
Institute of Pathology, Martin Luther University Halle-Wittenberg, Halle/Saale, Germany. helge.taubert@medizin.uni-halle.de
Thamm
B
B
Meye
A
A
Bartel
F
F
Rost
A K
AK
Heidenreich
D
D
John
V
V
Brandt
J
J
Bache
M
M
Würl
P
P
Schmidt
H
H
Riemann
D
D
D000293:Adolescent; D000328:Adult; D000368:Aged; D001171:Arthritis, Juvenile / Q000235:genetics* / Q000378:metabolism; D001172:Arthritis, Rheumatoid / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D002648:Child; D002675:Child, Preschool; D004252:DNA Mutational Analysis; D005091:Exons; D005260:Female; D015870:Gene Expression; D016158:Genes, p53; D006801:Humans; D007438:Introns; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism
D016159:Tumor Suppressor Protein p53