Effects of Leukotriene B(4) Receptor Antagonist on Experimental Autoimmune Uveoretinitis in Rats.
Purpose: We evaluated the potential of leukotriene B(4) receptor antagonist (LTB(4) RA) as an anti-inflammatory agent on experimental autoimmune uveoretinitis (EAU).Materials and Methods: LTB(4) RA was administered to Lewis rats twice a day on days 0-10, 0-5, and 6-10 after immunization. Rats treated on days 0-10 after immunization were subdivided into three groups according to the dosage of LTB(4) RA. The eyes were examined histopathologically, and the expression of CD 45 RC in CD(+)T cells was analyzed.Results: The inflammatory changes in the eyes of EAU were decreased in all groups treated with LTB(4) RA in a dose-dependent manner. The treatment with LTB(4) RA on days 0-10 after immunization achieved much higher uveitis suppression. The infiltration into eye tissues by neutrophils and lymphocytes was decreased by treatment with LTB(4) RA. In treated groups, the CD 45 RC(high) subset decreased in the induction phase of EAU as compared with the untreated group.Conclusion: The suppressive mechanisms of LT-B(4) RA on EAU may be dependent on suppression of the activation of neutrophils and CD 4(+)T cells.
Toriyama
S
Department of Ophthalmology, Yokohama City University School of Medicine, Yokohama, Japan