A novel monoclonal antibody, C41, reveals IL-13Ralpha1 expression by murine germinal center B cells and follicular dendritic cells.
Responsiveness to IL-13 involves at least two chains, IL-4Ralpha and IL-13Ralpha1. Although mouse B cells express IL-4Ralpha, little is known about their expression of IL-13Ralpha chains. To investigate this topic further, we have generated a monoclonal antibody (C41) specific for murine IL-13Ralpha1. Using C41, IL-13Ralpha1 expression was detected on germinal center (GC) B cells by flow cytometry and immunohistochemistry. In addition, IL-13Ralpha1 was observed on follicular dendritic cells, but not interdigitating dendritic cells in the T cell areas. Furthermore, resting B cells also expressed IL-13Ralpha1, and in the presence of IL-13 produced increased amounts of IgM in response to in vitro CD40 stimulation. However, C41 was unable to neutralize this bioactivity. The distribution of IL-13Ralpha1 on murine B cells and during GC reactions suggests a role for IL-13 during B cell differentiation.
Poudrier
J
J
Department of Immunology, Serono Pharmaceutical Research Institute, Geneva, Switzerland.
Graber
P
P
Herren
S
S
Berney
C
C
Gretener
D
D
Kosco-Vilbois
M H
MH
Gauchat
J F
JF
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology*; D020566:Dendritic Cells, Follicular / Q000276:immunology*; D053661:Interleukin-13 Receptor alpha1 Subunit; D051379:Mice; D018123:Receptors, Interleukin / Q000096:biosynthesis / Q000276:immunology*; D053660:Receptors, Interleukin-13
D000911:Antibodies, Monoclonal; C508183:Il13ra1 protein, mouse; D053661:Interleukin-13 Receptor alpha1 Subunit; D018123:Receptors, Interleukin; D053660:Receptors, Interleukin-13