Decreased zinc ion accumulation by the basidiomycete Lentinus edodes over-expressing L. edodes PriA gene.
The information that the deduced expression product of Lentinus edodes priA gene consists of N-terminal hydrophobic sequence, putative zinc-binding motifs and C-terminal membrane-binding-promoting unique sequence led us to analyze its function in L. edodes. Here L. edodes monokaryotic cells over-expressing priA gene were found to exhibit a remarkably decreased accumulation of zinc ion, indicating the involvement of the priA gene in regulation of the intracellular zinc concentration.
Ishizaki
T
T
Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Midori-ku, 226-8501, Yokohama, Japan.
Shishido
K
K
D001483:Base Sequence; D001692:Biological Transport; D015152:Blotting, Northern; D015139:Blotting, Southern; D018076:DNA, Complementary; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism*; D015870:Gene Expression; D005800:Genes, Fungal; D020074:Lentinula / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D008969:Molecular Sequence Data; D014170:Transformation, Genetic; D015032:Zinc / Q000378:metabolism*
D018076:DNA, Complementary; D005656:Fungal Proteins; C075074:priA protein, Lentinus edodes; D015032:Zinc