title: Structure of a family IIIa scaffoldin CBD from the cellulosome of Clostridium cellulolyticum at 2.2 A resolution.
abstract: The crystal structure of the family IIIa cellulose-binding domain (CBD) from the cellulosomal scaffoldin subunit (CipC) of Clostridium cellulolyticum has been determined. The structure reveals a nine-stranded jelly-roll topology which exhibits distinctive structural elements consistent with family III CBDs that bind crystalline cellulose. These include a well conserved calcium-binding site, a putative cellulose-binding surface and a conserved shallow groove of unknown function. The CipC CBD structure is very similar to the previously elucidated family IIIa CBD from the CipA scaffoldin of C. thermocellum, with some minor differences. The CipC CBD structure was also compared with other previously described CBD structures from families IIIc and IV derived from the endoglucanases of Thermomonospora fusca and Cellulomonas fimi, respectively. The possible functional consequences of structural similarities and differences in the shallow groove and cellulose-binding faces among various CBD families and subfamilies are discussed.
authors.affiliation: Faculty of Chemistry, The Weizmann Institute of Science, Rehovot, Israel. linda.shimon@weizmann.ac.il
authors.forename: L J
authors.initials: LJ
authors.lastname: Shimon
authors.forename: S
authors.initials: S
authors.lastname: Pagès
authors.forename: A
authors.initials: A
authors.lastname: Belaich
authors.forename: J P
authors.initials: JP
authors.lastname: Belaich
authors.forename: E A
authors.initials: EA
authors.lastname: Bayer
authors.forename: R
authors.initials: R
authors.lastname: Lamed
authors.forename: Y
authors.initials: Y
authors.lastname: Shoham
authors.forename: F
authors.initials: F
authors.lastname: Frolow
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry*; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism; D002352:Carrier Proteins / Q000737:chemistry*; D002482:Cellulose / Q000378:metabolism; D003013:Clostridium / Q000737:chemistry*; D018360:Crystallography, X-Ray; D008565:Membrane Proteins / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; C080369:CipA protein, Clostridium; C098847:CipC protein, Clostridium cellulolyticum; D008565:Membrane Proteins; D002482:Cellulose; D002118:Calcium