The human Hsp70B gene at the HSPA7 locus of chromosome 1 is transcribed but non-functional.
The human heat-inducible Hsp70B and Hsp70B' genes were co-localized to 1q23.1 by in situ hybridization. However, though transcripts from Hsp70B could be detected in heat-shocked cells, DNA sequence analyses of both the gene and cDNA copies of the mRNA indicate the gene is non-functional. Moreover, mouse homologues of Hsp70B/B' were not detected by Southern blot analysis, suggesting Hsp70B/B' arose from either Hsp70-1or Hsp70-2 after the divergence of mice and humans.
Parsian
A J
AJ
Department of Radiation Oncology, Washington University School of Medicine, St. Louis, MO 63108, USA.
Sheren
J E
JE
Tao
T Y
TY
Goswami
P C
PC
Malyapa
R
R
Van Rheeden
R
R
Watson
M S
MS
Hunt
C R
CR
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics*; D019143:Evolution, Molecular; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics* / Q000502:physiology*; D018869:Heat-Shock Response / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic / Q000235:genetics*; D014407:Tumor Cells, Cultured
D018840:HSP70 Heat-Shock Proteins; C491129:HSPA7 protein, human; D012333:RNA, Messenger