title: Glucose and the ATP paradox in yeast.
abstract: A sustained decrease in the intracellular ATP concentration has been observed when extra glucose was added to yeast cells growing aerobically under glucose limitation. Because glucose degradation is the main source of ATP-derived free energy, this is a counter-intuitive phenomenon, which cannot be attributed to transient ATP consumption in the initial steps of glycolysis. We present a core model for aerobic growth in which glucose supplies carbon, as well as free energy, for biosynthesis. With Metabolic Control Analysis and numerical simulations, we demonstrate that the decrease in the ATP concentration can be reproduced if the biosynthetic route is more strongly activated by carbon substrates than is the catabolic (ATP-producing) route.
authors.affiliation: Department of Molecular Cell Physiology, IMBS, BioCentrum Amsterdam, Free University, De Boelelaan 1087, NL-1081 HV Amsterdam, The Netherlands. oscar@bio.vu.nl
authors.forename: O J
authors.initials: OJ
authors.lastname: Somsen
authors.forename: M A
authors.initials: MA
authors.lastname: Hoeben
authors.forename: E
authors.initials: E
authors.lastname: Esgalhado
authors.forename: J L
authors.initials: JL
authors.lastname: Snoep
authors.forename: D
authors.initials: D
authors.lastname: Visser
authors.forename: R T
authors.initials: RT
authors.lastname: van der Heijden
authors.forename: J J
authors.initials: JJ
authors.lastname: Heijnen
authors.forename: H V
authors.initials: HV
authors.lastname: Westerhoff
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005947:Glucose / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
chemical_list: D000255:Adenosine Triphosphate; D005947:Glucose