Dehydroepiandrosterone prevents oxidative injury induced by transient ischemia/reperfusion in the brain of diabetic rats.
Both chronic hyperglycemia and ischemia/reperfusion (IR) cause an imbalance in the oxidative state of tissues. Normoglycemic and streptozotocin (STZ)-diabetic rats were subjected to bilateral carotid artery occlusion for 30 min followed by reperfusion for 60 min. Rats had either been treated with dehydroepiandrosterone (DHEA) for 7, 14, or 21 days (2 or 4 mg/day per rat) or left untreated. Oxidative state, antioxidant balance, and membrane integrity were evaluated in isolated synaptosomes. IR increased the levels of reactive species and worsened the synaptic function, affecting membrane Na/K-ATPase activity and lactate dehydrogenase release in all rats. The oxidative imbalance was much severer when transient IR was induced in STZ-diabetic rats. DHEA treatment restored H2O2, hydroxyl radical, and reactive oxygen species to close to control levels in normoglycemic rats and significantly reduced the level of all reactive species in STZ-diabetic rats. Moreover, DHEA treatment counteracted the detrimental effect of IR on membrane integrity and function: the increase of lactate dehydrogenase release and the drop in Na/K-ATPase activity were significantly prevented in both normoglycemic and STZ-diabetic rats. The results confirm that DHEA, an adrenal steroid that is synthesized de novo by brain neurons and astrocytes, possesses a multitargeted antioxidant effect. They also show that DHEA treatment is effective in preventing both derangement of the oxidative state and neuronal damage induced by IR in experimental diabetes.
Aragno
M
M
Department of Experimental Medicine and Oncology, General Pathology Section, University of Turin, Italy.
Parola
S
S
Brignardello
E
E
Mauro
A
A
Tamagno
E
E
Manti
R
R
Danni
O
O
Boccuzzi
G
G
D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use; D002545:Brain Ischemia / Q000150:complications* / Q000503:physiopathology; D002462:Cell Membrane / Q000502:physiology; D003687:Dehydroepiandrosterone / Q000008:administration & dosage / Q000097:blood / Q000627:therapeutic use*; D003921:Diabetes Mellitus, Experimental / Q000150:complications*; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D006861:Hydrogen Peroxide / Q000378:metabolism; D017665:Hydroxyl Radical / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation; D008297:Male; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D015427:Reperfusion Injury / Q000503:physiopathology / Q000517:prevention & control*; D000254:Sodium-Potassium-Exchanging ATPase; D013569:Synapses / Q000502:physiology; D013570:Synaptic Membranes / Q000737:chemistry
D000975:Antioxidants; D005231:Fatty Acids, Unsaturated; D017382:Reactive Oxygen Species; D017665:Hydroxyl Radical; D003687:Dehydroepiandrosterone; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; D000254:Sodium-Potassium-Exchanging ATPase