title: Double oxidation of D-xylose to D-glycero -pentos-2,3-diulose (2,3-diketo-D-xylose) by pyranose dehydrogenase from the mushroom Agaricus bisporus.
abstract: Pyranose dehydrogenase purified to homogeneity from the mycelia of the basidiomycete fungus Agaricus bisporus catalyzed the oxidation of D-xylose at C-2 to D-threo-pentos-2-ulose (2-keto-D-xylose) and successively at C-3 to D-glycero-pentos-2,3-diulose (2,3-diketo-D-xylose) using 1,4-benzoquinone as an electron acceptor. The sites of oxidation were deduced from the spectroscopic analysis (MS, NMR) of the N,N-diphenylhydrazone derivatives of the reaction products.
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague. volc@biomed.cas.cz
authors.forename: J
authors.initials: J
authors.lastname: Volc
authors.forename: P
authors.initials: P
authors.lastname: Sedmera
authors.forename: P
authors.initials: P
authors.lastname: Halada
authors.forename: V
authors.initials: V
authors.lastname: Prikrylová
authors.forename: D
authors.initials: D
authors.lastname: Haltrich
mesh_terms: D000364:Agaricus / Q000201:enzymology*; D002237:Carbohydrate Dehydrogenases / Q000302:isolation & purification / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D006835:Hydrazones / Q000737:chemistry; D007659:Ketones / Q000737:chemistry / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010084:Oxidation-Reduction; D014994:Xylose / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism*
chemical_list: C421921:2,3-diketoxylose; D006835:Hydrazones; D007659:Ketones; D014994:Xylose; D002237:Carbohydrate Dehydrogenases