Autolysis during in vitro tracheary element differentiation: formation and location of the perforation.
Tracheary elements differentiated from isolated Zinnia: mesophyll cells were observed at various times of culture under a scanning electron microscope. Perforation occurred on the primary wall at one of the longitudinal ends in single tracheary elements. In double tracheary elements, which both of two cells derived from a single cell differentiated into, the pore opened on the primary walls both at the junction of the two tracheary elements and at a longitudinal end of one of the two tracheary elements. These results suggest not only that a single tracheary element has its own program to form a perforation at one end without being affected by neighboring cells, but also that isolated cells indeed hold some traces of polarity and cell-cell communication.
Nakashima
J
J
Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, 113-0033 Japan. jean@biol.s.u-tokyo.ac.jp
Takabe
K
K
Fujita
M
M
Fukuda
H
H
D017209:Apoptosis; D019659:Asteraceae / Q000166:cytology / Q000502:physiology / Q000648:ultrastructure; D002454:Cell Differentiation / Q000502:physiology*; D008855:Microscopy, Electron, Scanning; D018515:Plant Leaves / Q000166:cytology / Q000502:physiology / Q000648:ultrastructure; D013997:Time Factors