[Mechanism of acceleration of acid hemolysis of the E-form of serum albumin].
The influence of human and bovine serum albumin on the proton-mediated lysis of human, rabbit and sheep erythrocytes was studied. An essential acceleration of hemolysis (2-7-fold) by the action of albumin was observed only at pH values below 4.0, i.e. at values at which the transition of albumin to the E conformation occurs. It was shown in the experiments with fatty-acid-free albumin and the protein loaded by palmitate that the accelerating effect of albumin was due to the hemolytic action of fatty acids released from the protein. As a result of the proton potentiation of detergent-dependent hemolysis, fatty acids released from E-albumin become more potent hemolytic agents.
Galebskaia
L V
LV
Pavlov Medical University, St. Petersburg, Russia.
Denisenko
A D
AD
Simkina
N B
NB
Tarasova
Iu V
IuV
Tolochko
S I
SI
D000143:Acids; D000818:Animals; D005227:Fatty Acids / Q000378:metabolism; D006461:Hemolysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D011817:Rabbits; D012709:Serum Albumin / Q000378:metabolism*; D012756:Sheep
D000143:Acids; D005227:Fatty Acids; D012709:Serum Albumin