Use of automated solid-phase extraction equipment for the determination of isoniazid in milk.
The possibility of using automated equipment for solid phase extraction of isoniazid from milk has been investigated. This equipment provides two noteworthy advantages: it shortens the time of analysis and avoids SPE column plugging, stabilizing flow rates. After setting the correct programme on software controlling the equipment, some validation parameters have been determined to obtain recovery and repeatability data. The mean recovery for spiked samples resulted in the range 75-95%, the standard error ranged from -5 to -20% while the RSD varied from 2 to 22%. Therefore the method can be considered reliable and fast.
Grasso
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Dipartimento di Chimica, Istituto Zooprofilattico Sperimentale del Mezzogiorno (IZSM), Portici, Italy.
Scarano
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G
Imparato
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E
Arace
O
O
Oliviero
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G
D000818:Animals; D000995:Antitubercular Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000592:standards; D004350:Drug Residues / Q000032:analysis*; D007538:Isoniazid / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D012680:Sensitivity and Specificity
D000995:Antitubercular Agents; D007538:Isoniazid