Genomic organization, chromosomal localization, and the complete 22 kb DNA sequence of the human GCMa/GCM1, a placenta-specific transcription factor gene.
The genomic sequence of the human GCMa/GCM1 gene, a mammalian homologue of Drosophila melanogaster GCM, was determined. Drosophila GCM is a neural transcription factor that regulates glial cell fate. The mammalian homolog however, is a placenta-specific transcription factor that is necessary for placental development. The 22 kb DNA sequence spanning the GCMa gene contains six exons and five introns, encoding a 2.8 kb cDNA. Overall genomic organization is similar for the human and mouse. Several potential binding sites for transcription factors like GATA, Oct-1, and bHLH proteins were found in the 5'-flanking region of the human gene. A DNA motif for GCM protein binding exists in the 5'-flanking region that is highly homologous with that of the mouse gene. The location of this gene was mapped to chromosome 6 using fluorescence in situ hybridization.
Yamada
K
K
Institute for Comprehensive Medical Science, Fujita Health University, School of Medicine, Toyoake, Aichi, Japan. kyamada@fujita-hu.ac.jp
Ogawa
H
H
Tamiya
G
G
Ikeno
M
M
Morita
M
M
Asakawa
S
S
Shimizu
N
N
Okazaki
T
T
D000818:Animals; D001483:Base Sequence; D051792:Basic Helix-Loop-Helix Proteins; D001665:Binding Sites; D002874:Chromosome Mapping; D002896:Chromosomes, Human, Pair 6; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000378:metabolism; D005091:Exons; D050989:GATA2 Transcription Factor; D050990:GATA3 Transcription Factor; D015723:Gene Library; D051863:Host Cell Factor C1; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007438:Introns; D051379:Mice; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics*; D009687:Nuclear Proteins; D050811:Octamer Transcription Factor-1; D010920:Placenta / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D015534:Trans-Activators / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D014327:Trophoblasts
D051792:Basic Helix-Loop-Helix Proteins; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D050989:GATA2 Transcription Factor; D050990:GATA3 Transcription Factor; D051863:Host Cell Factor C1; D009479:Neuropeptides; D009687:Nuclear Proteins; D050811:Octamer Transcription Factor-1; D015534:Trans-Activators; D014157:Transcription Factors; C494711:GATA2 protein, human; C494749:GATA3 protein, human; C491089:GCM1 protein, human; C494712:Gata2 protein, mouse; C494748:Gata3 protein, mouse; C101631:Gcm1 protein, mouse; C497692:HCFC1 protein, human; C497693:Hcfc1 protein, mouse; C494165:POU2F1 protein, human; C494166:Pou2f1 protein, mouse