Evidence for a parapox ovis virus-associated superantigen.
As shown in a number of species, susceptibility to infectious diseases can be efficiently reduced following application of inactivated parapox ovis viruses (iPPOV). However, the basic mechanism for this stimulating capacity of iPPOV remains unclear. When analyzed, the interaction of iPPOV with porcine peripheral blood mononuclear cells was seen to involve T helper cells as the main target cell population responding to iPPOV. These cells displayed a strong proliferation, and were the major source for the observed increased levels of IL-2. Activation of the T helper cells was MHC class II dependent, but not MHC class II restricted: cellular processing of iPPOV was not required for presentation by autologous, allogeneic or xenogeneic MHC class II molecules. Furthermore, CD3 and CD4 molecules were involved in the stimulation, indicating a receptor-mediated activation of T helper cells. The results demonstrated typical characteristics of a superantigen-induced response providing evidence for a viral component within PPOV functioning as superantigen(s) in swine.
Fachinger
V
V
Institute of Immunology, Federal Research Centre for Virus Diseases of Animals, Tübingen, Germany.
Schlapp
T
T
Saalmüller
A
A
D000818:Animals; D017849:Animals, Inbred Strains; D017951:Antigen Presentation; D000938:Antigen-Presenting Cells / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D017252:CD3 Complex / Q000276:immunology; D015704:CD4 Antigens / Q000276:immunology; D002460:Cell Line; D002478:Cells, Cultured; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D016130:Immunophenotyping; D007376:Interleukin-2 / Q000378:metabolism; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D009923:Orf virus / Q000276:immunology*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology; D013045:Species Specificity; D018089:Superantigens / Q000276:immunology*; D013552:Swine; D018417:Th1 Cells / Q000276:immunology* / Q000378:metabolism
D000956:Antigens, Viral; D017252:CD3 Complex; D015704:CD4 Antigens; D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2; D016693:Receptors, Antigen, T-Cell, alpha-beta; D018089:Superantigens