[Overall genomic DNA methylation in relation to estrogen].
Overall genomic DNA methylation was analyzed using enzymatic digestion into nucleotides, 32P postlabeling, two-dimensional thin-layer chromatography on cellulose plates and phosphobioimaging quantitation, in relation to immunohistochemically measured estrogen (ER) and progesterone receptor (PR) status of 15 uterine cancers. Mean 5-methyldeoxycytosine (m5dC) content did not differ between ER-positive and ER-negative neoplasms. Highest values of m5dC were noted both in ER-negative and ER-positive tumors. Additionally, there was no low DNA methylation in ER negative uterine cancer tissues. Decrease of the overall genomic DNA methylation could be related to the increase of ER/PR ratio, however it was not significant in our investigation. The potential role of steroid receptors status in uterine cancer tissue is discussed.
Postawski
K
K
Kliniki Ginekologii Operacyjnej II Katedry Połoznictwa i Chorób Kobiecych AM w Lublinie.
Olech-Fudali
E
E
Jakowicki
J A
JA
Korobowicz
E
E
Keith
G
G
Baranowski
W
W
D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D019175:DNA Methylation; D015254:DNA Modification Methylases / Q000378:metabolism; D005260:Female; D006801:Humans; D008875:Middle Aged; D011960:Receptors, Estrogen / Q000378:metabolism*; D011980:Receptors, Progesterone / Q000378:metabolism*; D014594:Uterine Neoplasms / Q000378:metabolism* / Q000473:pathology*; D014599:Uterus / Q000378:metabolism* / Q000473:pathology
D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D015254:DNA Modification Methylases