Selective autophagy of clofibrate-induced rat liver peroxisomes. Cytochemistry and immunocytochemistry on tissue specimens and on fractions obtained by Nycodenz density gradient centrifugation.
Following short treatments with peroxisomal proliferators rodent liver undergoes a significant increase in the peroxisomal population, accompanied by specific induction of some peroxisomal enzymes; both phenomena are reversible and in a few days after drug withdrawal the control parameters are recovered. The involvement of lysosomal system in removal of proliferated peroxisomes has been widely suggested, and the autophagic phenomenon was mainly investigated in experimental conditions in which the administration of lysosomotropic drugs or, more generally, of digestive process inhibitors caused an accumulation of autophagic vacuoles. In the present research the removal of clofibrate-induced rat liver peroxisomes was investigated under physiological conditions, i.e. in the absence of drugs interfering with the autophagic process. In a previous paper the lysosomal involvement in peroxisomal removal was suggested on the basis both of biochemical and cytochemical-immunocytochemical data. In the present paper the autophagic vacuoles and autolysosomes involved in the digestion of excess peroxisomes are more extensively described, mainly by means of colloidal gold immunocytochemistry, carried out also on density gradient subfractions.
Nardacci
R
R
Department of Cellular and Developmental Biology, University of Rome La Sapienza, Italy.
Sartori
C
C
Stefanini
S
S
D000135:Acid Phosphatase / Q000032:analysis / Q000378:metabolism; D000818:Animals; D001343:Autophagy; D015415:Biomarkers / Q000032:analysis; D002374:Catalase / Q000032:analysis / Q000378:metabolism; D002402:Cathepsin D / Q000032:analysis / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D002994:Clofibrate / Q000494:pharmacology*; D015151:Immunoblotting; D007150:Immunohistochemistry; D007472:Iohexol; D008099:Liver / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D008247:Lysosomes / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D020675:Peroxisomes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D013347:Subcellular Fractions / Q000201:enzymology / Q000378:metabolism / Q000648:ultrastructure; D014617:Vacuoles / Q000378:metabolism / Q000648:ultrastructure
D015415:Biomarkers; D007472:Iohexol; D002374:Catalase; D000135:Acid Phosphatase; D002402:Cathepsin D; D002994:Clofibrate