High-performance liquid chromatographic determination with photodiode array detection of ellagic acid in fresh and processed fruits.
A high-performance liquid chromatographic (HPLC) procedure based on an isocratic elution with photodiode array detection has been developed for a simple and rapid determination of ellagic acid (EA) in fresh and processed fruits. The homogenized sample was refluxed with methanol and then the extract was refined using a solid-phase cartridge before HPLC. We analyzed EA in 40 kinds of fresh fruits and 11 kinds of processed fruits by the developed method. EA was found in several berries, fueijoa, pineapple and pomegranate. This is the first occurrence of the detection of EA in bayberry, fueijoa and pineapple.
Amakura
Y
Y
National Institute of Health Sciences, Osaka Branch, Japan. amakura@nihs.go.jp
Okada
M
M
Tsuji
S
S
Tonogai
Y
Y
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004610:Ellagic Acid / Q000032:analysis*; D005638:Fruit / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D004610:Ellagic Acid