title: Generation of deletion and point mutations with one primer in a single cloning step.
authors.affiliation: University of Michigan Medical School, Ann Arbor, USA.
authors.forename: O
authors.initials: O
authors.lastname: Makarova
authors.forename: E
authors.initials: E
authors.lastname: Kamberov
authors.forename: B
authors.initials: B
authors.lastname: Margolis
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D017931:DNA Primers / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics; D016297:Mutagenesis, Site-Directed; D010957:Plasmids / Q000235:genetics; D017354:Point Mutation / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011933:Reagent Kits, Diagnostic; D017384:Sequence Deletion / Q000235:genetics*
chemical_list: D017931:DNA Primers; D011933:Reagent Kits, Diagnostic; D004262:DNA Restriction Enzymes