Cell-type dependence of stability modulation of lectin-initiated contacts by impairment of multivalent carbohydrate binding and intracellular signaling.
Initial ligand selection and the intermolecular spatial arrangement of glycan-lectin complexes are assumed to be essential to induce formation of stable cell aggregates by a lectin. To distinguish effects of these two processes, the tetrameric mistletoe lectin and its isolated B-chain were used. A reduced impact of multivalency for Ehrlich ascites tumor cells in contrast to rat thymocytes was revealed. Signaling is thus initiated in a cell-type-dependent manner. Using selective metabolic inhibitors to reduce signal transfer for aggregate stability, decrease in cellular SH-group level was shown to be a common effect accompanying suppression of lectin-dependent aggregate stability. The results underscore an intrinsic variability in the relative importance of lectin-dependent glycan aggregation on the cell surface for triggering post-binding lectin effects.
Timoshenko
A V
AV
Institute of Photobiology, National Academy of Sciences of Belarus, Minsk. timoshenko@phys.bsu.unibel.by
Gorudko
I V
IV
André
S
S
Gabius
H J
HJ
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism / Q000473:pathology; D002449:Cell Aggregation / Q000187:drug effects*; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005453:Fluorescence; D007700:Kinetics; D007785:Lactose / Q000494:pharmacology; D008297:Male; D051379:Mice; D009928:Organ Specificity; D028321:Plant Preparations; D010940:Plant Proteins; D011134:Polysaccharides / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D054790:Ribosome Inactivating Proteins, Type 2; D015398:Signal Transduction / Q000187:drug effects*; D013438:Sulfhydryl Compounds / Q000032:analysis; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D014118:Toxins, Biological / Q000378:metabolism / Q000494:pharmacology*
D004791:Enzyme Inhibitors; D028321:Plant Preparations; D010940:Plant Proteins; D011134:Polysaccharides; D054790:Ribosome Inactivating Proteins, Type 2; D013438:Sulfhydryl Compounds; D014118:Toxins, Biological; C009674:ribosome inactivating protein, Viscum; D007785:Lactose