title: Fate of atrazine and chlorpyrifos during solid state fermentation--examination of processes.
abstract: Solid state fermentation (SSF) was investigated as a means to dispose of two commonly used pesticides, chlorpyrifos (O,O-diethyl O-(3,5,6-trichloro-2-pyridyl) phosphorothioate) and atrazine (2-chloro-4-ethylamino-6-isopropylamino-1,3,5-triazine). SSF experiments were carried out in bench-scale bioreactors (equipped with CO2 and volatile organic traps) containing a mixture of lignocellulosic materials and a radiolabeled pesticide. Ethyl acetate-extractable, alkali soluble, and alkali insoluble fractions were evaluated for radioactivity following a 60-d incubation period at 40 degrees C. The majority of the [2,6-pyridyl-14C]chlorpyrifos was associated with the ethyl acetate extract (about 74%), 17% was trapped as organic volatiles by polyurethane foam traps and < 0.5% of the chlorpyrifos was mineralized to CO2. Only small amounts of the radioactivity were associated with alkali soluble (0.0003%) and alkali insoluble (0.3%) fractions. In the [14C-U-ring]atrazine bioreactors, very little of the radioactivity volatilized (<0.5%) and less than 0.5% was mineralized to CO2. Approximately 57% of the applied radioactivity was associated with the ethyl acetate extract while 9% and 24% of the radioactivity was associated with the alkali soluble (humic and fulvic acids) and alkali insoluble fractions, respectively. Possible reaction mechanisms by which covalent bonds could be formed between atrazine (or metabolites) and humic substances were investigated. The issue of bound atrazine residue (alkali soluble fraction) was at least partially resolved. Oxidative coupling experiments revealed that formation of covalent bond linkages between amino substituent groups of atrazine residue and humic substances is highly unlikely.
authors.affiliation: Aventis CropScience, Research Triangle Park, North Carolina 27709, USA.
authors.forename: D N
authors.initials: DN
authors.lastname: Judge
authors.forename: D E
authors.initials: DE
authors.lastname: Mullins
authors.forename: D F
authors.initials: DF
authors.lastname: Berry
authors.forename: H L
authors.initials: HL
authors.lastname: Walker
mesh_terms: D001280:Atrazine / Q000737:chemistry*; D004390:Chlorpyrifos / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D006540:Herbicides / Q000737:chemistry*; D007306:Insecticides / Q000737:chemistry*; D010573:Pesticide Residues; D012037:Refuse Disposal / Q000379:methods*
chemical_list: D006540:Herbicides; D007306:Insecticides; D010573:Pesticide Residues; D004390:Chlorpyrifos; D001280:Atrazine