Morphological changes in the nucleus and actin cytoskeleton in the process of Fas-induced apoptosis in Jurkat T cells.
To investigate the early event of apoptosis, we monitored the morphological changes in the early stage of Fas-induced apoptosis in the human T-cell lymphoma cell line Jurkat, using confocal microscopy. Morphological changes in the nuclei were observed from 30 min after stimulation, and preceded the changes in the cytoskeleton. This kind of change was enhanced in the presence of EGTA but decreased in the presence of dihydrocytochalasin B, without any changes in caspase-3 activation. During the changes in shape of the cells, the actin cytoskeleton collapsed and shrank in the center. Even though nuclei also changed their shapes in apoptotic cells, they were partially TUNEL-negative, suggesting that they were not yet damaged at the DNA level. Our results suggest that, in the process of apoptosis in Jurkat cells, cell nuclei and cytoskeleton are changed first, then membrane blebbing and caspase-3 activation occur, and fragmentation of chromosomal DNA is last.
Maruyama
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Laboratory of Molecular Oncology, Tsukuba Life Science Center, The Institute of Physical and Chemical Research (RIKEN), Ibaraki, Japan.
Irie
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Sato
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TA
D000199:Actins / Q000648:ultrastructure; D017209:Apoptosis; D002467:Cell Nucleus / Q000648:ultrastructure*; D003599:Cytoskeleton / Q000648:ultrastructure*; D006801:Humans; D019169:Jurkat Cells; D018613:Microscopy, Confocal; D013601:T-Lymphocytes / Q000473:pathology*; D019014:fas Receptor
D000199:Actins; D019014:fas Receptor