HD-PTP: A novel protein tyrosine phosphatase gene on human chromosome 3p21.3.
A human cDNA encoding a novel protein tyrosine phosphatase has been isolated. The phosphatase has unique features in its domain structure: a "Zn-hand" domain containing several SH3-binding motifs, a tyrosine phosphatase domain, a C-terminal PEST motif, and an N-terminal domain similar to yeast BRO1, an apoptosis-related mammalian AIP1 and to a RHO-binding protein, Rhophilin. The gene is located at chromosome 3p21.3, an area frequently deleted in many types of cancer, especially within the functionally defined narrow region. The gene may be a human homolog of the rat PTP-TD14 gene reported by others, which can suppress H-ras-mediated transformation. We identified a hemizygous missense mutation in a lung cancer cell line. Thus, the phosphatase gene may be a candidate for one of the tumor suppressor genes located on 3p21.3.
Toyooka
S
S
Department of Surgery(II), Okayama University Medical School, Okayama, 700-8558, Japan.
Ouchida
M
M
Jitsumori
Y
Y
Tsukuda
K
K
Sakai
A
A
Nakamura
A
A
Shimizu
N
N
Shimizu
K
K
D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002893:Chromosomes, Human, Pair 3; D018076:DNA, Complementary; D005819:Genetic Markers; D006801:Humans; D019656:Loss of Heterozygosity; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D017433:Protein Structure, Secondary; D017027:Protein Tyrosine Phosphatases / Q000737:chemistry / Q000235:genetics*; D054558:Protein Tyrosine Phosphatases, Non-Receptor; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D005819:Genetic Markers; D011994:Recombinant Proteins; C114102:PTPN23 protein, human; D017027:Protein Tyrosine Phosphatases; D054558:Protein Tyrosine Phosphatases, Non-Receptor; C520791:Ptpn23 protein, rat