Interleukin 12 as an adjuvant to WI-1 adhesin immunization augments delayed-type hypersensitivity, shifts the subclass distribution of immunoglobulin G antibodies, and enhances protective immunity to Blastomyces dermatitidis infection.
Cell-mediated immunity is pivotal in host resistance to Blastomyces dermatitidis infection. Immunization of mice with the WI-1 adhesin enhances resistance against experimental pulmonary infection but elicits features of a mixed T-helper-cell immune response. Immune mice acquire delayed-type hypersensitivity (DTH) but also high titers of WI-1-specific immunoglobulin G1 (IgG1) and IgG2b, a result indicative of T-helper-2 cellular immunity. We report that interleukin-12, used as an adjuvant for WI-1 immunization, augments DTH, shifts the balance of the T-helper phenotype toward Th1, and enhances resistance to B. dermatitidis infection.
Wüthrich
M
M
Department of Pediatrics, University of Wisconsin Medical School, University of Wisconsin Hospital and Clinics, Madison, Wisconsin 53792, USA.
Finkel-Jiminez
B E
BE
Klein
B S
BS
D018829:Adhesins, Bacterial / Q000276:immunology*; D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000908:Antibodies, Fungal / Q000097:blood*; D001758:Blastomyces / Q000276:immunology*; D001759:Blastomycosis / Q000517:prevention & control*; D006968:Hypersensitivity, Delayed / Q000209:etiology*; D007114:Immunization; D007074:Immunoglobulin G / Q000145:classification*; D018664:Interleukin-12 / Q000494:pharmacology*; D051379:Mice
D018829:Adhesins, Bacterial; D000276:Adjuvants, Immunologic; D000908:Antibodies, Fungal; D007074:Immunoglobulin G; D018664:Interleukin-12