Mapping protein-protein interaction domains using ordered fragment ladder far-western analysis of hexahistidine-tagged fusion proteins.
Burgess
R R
RR
McArdle Laboratory for Cancer Research, University of Wisconsin, Madison 53706, USA.
Arthur
T M
TM
Pietz
B C
BC
D000595:Amino Acid Sequence; D015153:Blotting, Western / Q000379:methods; D003001:Cloning, Molecular / Q000379:methods; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D019811:Hydroxylamine; D007202:Indicators and Reagents; D009842:Oligopeptides / Q000737:chemistry; D021122:Protein Subunits; D011993:Recombinant Fusion Proteins / Q000032:analysis* / Q000096:biosynthesis; D015183:Restriction Mapping; D013820:Thermolysin
D007202:Indicators and Reagents; D009842:Oligopeptides; D021122:Protein Subunits; D011993:Recombinant Fusion Proteins; D019811:Hydroxylamine; D012321:DNA-Directed RNA Polymerases; D013820:Thermolysin