title: Maternal control of sex ratio in Rana rugosa: evidence from DNA sexing.
abstract: Parental control of primary sex ratio has been reported in a mammal (red deer), some birds, and a snake. However, it remains uncertain whether other vertebrates including Amphibia can control sex ratio. In this paper, we examined the possibility in a wild population of the Japanese frog Rana rugosa which has female heterogamety. Sex ratios of their eggs were determined using DNA markers. The eggs were sampled in the field from May to August in 1998. Each egg was then sexed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using a sex-specific DNA marker. The result showed a male bias early in the season which changed to a female bias later, suggesting that females of R. rugosa can control the primary sex ratio.
authors.affiliation: Department of Biology, Faculty of Science, Kyushu University, Fukuoka 812-8581, Japan. ysakiscb@mbox.nc.kyushu-u.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Sakisaka
authors.forename: T
authors.initials: T
authors.lastname: Yahara
authors.forename: I
authors.initials: I
authors.lastname: Miura
authors.forename: E
authors.initials: E
authors.lastname: Kasuya
mesh_terms: D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D017753:Ecosystem; D005260:Female; D008297:Male; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011898:Ranidae / Q000235:genetics*; D012621:Seasons; D012744:Sex Ratio
chemical_list: D017931:DNA Primers; D004247:DNA