High-performance liquid chromatographic method for determination of DDT and its degradation products in rat plasma, liver and brain: validation and application to a pharmacokinetic study.
A sensitive and reliable high-performance liquid chromatographic (HPLC) method, using a solid-phase extraction (SPE), was established and validated for determination of p,p'-DDT [1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane] and its metabolite p,p'-DDE [1,1-(2,2-dichloroethanylidene)-bis(4-chlorobenzene)] in rat plasma, liver and brain. After being diluted with water, plasma, liver and brain samples were applied to a solid-phase extraction C18 cartridge. The extraction containing p,p'-DDT and p,p'-DDE from the cartridge were cleaned-up using a Florisil Sep-Pak cartridge. The samples were analyzed by HPLC using UV detection at 238 nm. The limit of detection for p,p'-DDT and p,p'-DDE was 0.1 mg kg(-1) liver or brain and 0.1 mg l(-1) plasma. For six replicate samples at 40, 4 and 0.2 mg kg(-1), intra-day precision values were within 4.9% for plasma, 6.4% for liver, and 9.7% for brain. Inter-day precision values at 4 mg kg(-1) were within 8.2% for plasma and tissues. The method performances were shown to be selective for p,p'-DDT and p,p'-DDE, and linear over the range 0.04-12 mg kg(-1) (mg L(-1) for plasma). The absolute recoveries of p,p'-DDT and p,p'-DDE in rat plasma and tissues were over 92%. The method was proved to be applicable to the pharmacokinetic study of DDT in rats after a single oral administration.
Tomiyama
N
N
The Institute of Environmental Toxicology, Chemistry Division, Ibaraki, Japan.
Tsuji
H
H
Watanabe
M
M
Takeda
M
M
Harada
T
T
Kobayashi
H
H
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003634:DDT / Q000097:blood / Q000493:pharmacokinetics*; D008099:Liver / Q000378:metabolism*; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D003634:DDT