Analysis of parity between protein-based electrophoretic methods for the characterization of oral Candida species.
Electrophoretic studies of multilocus-enzymes (MLEE) and whole-cell protein (SDS-PAGE) were carried out in order to evaluate the parity between different methods for the characterization of five Candida species commonly isolated from oral cavity of humans by numerical taxonomy methods. The obtained data revealed that sodium dodecyl sulfate polyacrylamide gel electrophoresis is more efficient in grouping strains in their respective species while MLEE has much limited resolution in organizing all strains in their respective species-specific clusters. MLEE technique must be regarded for surveys in which just one species of Candida is involved.
Rosa
E A
EA
Laboratório de Microbiologia e Imunologia, Faculdade de Odontologia de Piracicaba, Unicamp, 13414-900 Piracicaba, SP, Brasil.
Rosa
R T
RT
Pereira
C V
CV
Boriollo
M F
MF
Höfling
J F
JF
D002175:Candida / Q000737:chemistry* / Q000145:classification; D004586:Electrophoresis / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004798:Enzymes / Q000032:analysis*; D005656:Fungal Proteins / Q000032:analysis*; D006801:Humans; D009055:Mouth / Q000382:microbiology*
D004798:Enzymes; D005656:Fungal Proteins