title: Maturation of envelope-specific antibody responses to linear determinants in monkeys inoculated with attenuated SIV.
abstract: We have previously used a panel of quantitative and qualitative serological assays to define a lengthy and complex maturation of envelope-specific antibody responses in monkeys experimentally infected with attenuated simian immunodeficiency virus (SIV) that is closely associated with the temporal development of enduring and protective immunity to experimental virus challenge. To elucidate in more detail the changes in antibody specificity associated with this maturation, we describe here 'domain-specific' serological studies to characterize the evolution of antibody responses to defined linear determinants of the SIV envelope protein. The results of these studies reveal for the first time distinguishing differences in the evolution of antibody populations to distinct envelope peptide segments, as determined by measurements of antibody titer and avidity, indicating different patterns of antibody maturation to distinct linear envelope antigenic determinants. Thus, these data demonstrate the potential for domain-specific serology to produce a high-resolution characterization of SIV-specific antibody responses that can be used to evaluate experimental vaccine responses and to identify potential immune correlates of protection.
authors.affiliation: Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, PA 15261, USA.
authors.forename: K S
authors.initials: KS
authors.lastname: Cole
authors.forename: M J
authors.initials: MJ
authors.lastname: Paliotti
authors.forename: M
authors.initials: M
authors.lastname: Murphey-Corb
authors.forename: R C
authors.initials: RC
authors.lastname: Montelaro
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000917:Antibody Formation; D000918:Antibody Specificity; D015699:HIV Envelope Protein gp120 / Q000737:chemistry / Q000276:immunology*; D008253:Macaca mulatta; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000276:immunology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D012191:Retroviridae Proteins / Q000737:chemistry / Q000276:immunology; D018449:SAIDS Vaccines / Q000276:immunology*; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology*; D015302:Simian Immunodeficiency Virus / Q000276:immunology*; D013997:Time Factors; D014613:Vaccines, Attenuated / Q000276:immunology*; D014759:Viral Envelope Proteins
chemical_list: D000914:Antibodies, Viral; D015699:HIV Envelope Protein gp120; D008562:Membrane Glycoproteins; D010446:Peptide Fragments; D012191:Retroviridae Proteins; D018449:SAIDS Vaccines; C082614:SIV envelope protein gp41; D014613:Vaccines, Attenuated; D014759:Viral Envelope Proteins; C069623:gp120 protein, Simian immunodeficiency virus