Endoglucanase activity and relative expression of glycoside hydrolase genes of Fibrobacter succinogenes S85 grown on different substrates.
The endoglucanase activity of cells and extracellular culture fluid of Fibrobacter succinogenes S85 grown on glucose, cellobiose, soluble polysaccharides (beta-glucan, lichenan) and intact plant polysaccharides, was compared. The specific activity of cells grown on cellulose or forages was 6- to 20-fold higher than that of cells grown on soluble substrates, suggesting an induction of endoglucanases by the insoluble substrates. The ratios of cells to extracellular culture fluid endoglucanase activities measured in cultures grown on sugars or insoluble polysaccharides suggested that the endoglucanases induced by the insoluble polysaccharides remained attached to the cells. The mRNA of all the F. succinogenes glycoside hydrolase genes sequenced so far were then quantified in cells grown on glucose, cellobiose or cellulose. The results show that all these genes were transcribed in growing cells, and that they are all overexpressed in cultures grown on cellulose. Endoglucanase-encoding endB and endA(FS) genes, and xylanase-encoding xynC gene appeared the most expressed genes in growing cells. EGB and ENDA are thus likely to play a major role in cellulose degradation in F. succinogenes.
Béra-Maillet
C
C
Laboratoire de Microbiologie, INRA, Centre de Clermont-Ferrand-Theix, 63122, Saint-Genès-Champanelle, France.
Gaudet
G
G
Forano
E
E
D001426:Bacterial Proteins / Q000096:biosynthesis*; D002475:Cellobiose; D002480:Cellulase / Q000378:metabolism*; D003470:Culture Media; D017931:DNA Primers; D015971:Gene Expression Regulation, Enzymologic; D005947:Glucose; D006026:Glycoside Hydrolases / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D014312:Trisaccharides
D001426:Bacterial Proteins; D003470:Culture Media; D017931:DNA Primers; D012333:RNA, Messenger; D014312:Trisaccharides; D002475:Cellobiose; D006026:Glycoside Hydrolases; D002480:Cellulase; D005947:Glucose