title: Modulation of fibroblast-mediated cartilage degradation by articular chondrocytes in rheumatoid arthritis.
abstract: OBJECTIVE
  To determine the role of chondrocytes and factors released from chondrocytes in cartilage destruction by fibroblast-like synoviocytes (FLS) derived from patients with rheumatoid arthritis (RA).
  
  
  METHODS
  RA FLS from 2 patients were implanted into SCID mice, together with fresh articular cartilage or with cartilage that had been stored for 24 hours at 4 degrees C or at 37 degrees C. The invasion of the same RA FLS into the fresh and stored cartilage was compared histologically using a semiquantitative scoring system. In addition, we investigated whether protein synthesis in chondrocytes affects the invasion of RA FLS in vitro. A 3-dimensional cartilage-like matrix formed by cultured chondrocytes was labeled with 35S. After formation of the cartilage-like matrix, protein synthesis was blocked with cycloheximide. The invasion of RA FLS from 6 patients into cycloheximide-treated and untreated matrix was assessed by measuring the released radioactivity in coculture with and without interleukin-1beta (IL-1beta) and tumor necrosis factor alpha (TNFalpha).
  
  
  RESULTS
  The SCID mouse experiments showed a significant invasion of RA FLS into the cartilage (overall mean score 3.2) but revealed significant differences when the invasion of the same RA FLS into fresh and stored cartilage was compared. RA FLS that were implanted with fresh articular cartilage showed a significantly higher invasiveness than those implanted with pieces of cartilage that had been stored for 24 hours (overall mean score 2.3). Storage at 37 degrees C and 4 degrees C resulted in the same reduction of invasion (35% and 37%, respectively). In the in vitro experiments, RA FLS rapidly destroyed the cartilage-like matrix. Blocking of chondrocyte protein biosynthesis significantly decreased the invasion of RA FLS, as shown by a decreased release of radioactivity. Addition of IL-1beta, but not TNFalpha, to the cocultures partially restored the invasiveness of RA FLS.
  
  
  CONCLUSIONS
  These data underline the value of the SCID mouse in vivo model of rheumatoid cartilage destruction and demonstrate that chondrocytes contribute significantly to the degradation of cartilage by releasing factors that stimulate RA FLS. Among those, IL-1beta-mediated mechanisms might be of particular importance.
authors.affiliation: WHO Collaborating Center for Molecular Biology and Novel Therapeutic Strategies for Rheumatic Diseases, University Hospital, Zurich, Switzerland.
authors.forename: T
authors.initials: T
authors.lastname: Pap
authors.forename: W H
authors.initials: WH
authors.lastname: van der Laan
authors.forename: K R
authors.initials: KR
authors.lastname: Aupperle
authors.forename: R E
authors.initials: RE
authors.lastname: Gay
authors.forename: J H
authors.initials: JH
authors.lastname: Verheijen
authors.forename: G S
authors.initials: GS
authors.lastname: Firestein
authors.forename: S
authors.initials: S
authors.lastname: Gay
authors.forename: M
authors.initials: M
authors.lastname: Neidhart
mesh_terms: D000818:Animals; D001172:Arthritis, Rheumatoid / Q000473:pathology*; D002358:Cartilage, Articular / Q000166:cytology / Q000378:metabolism*; D002465:Cell Movement / Q000187:drug effects; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism; D016207:Cytokines / Q000494:pharmacology; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D014176:Protein Biosynthesis; D011506:Proteins / Q000187:drug effects; D013583:Synovial Membrane / Q000166:cytology
chemical_list: D016207:Cytokines; D011506:Proteins