End-specific covalent photo-dependent immobilisation of synthetic DNA to paramagnetic beads.
A novel approach for light-dependent covalent immobilisation of synthetic DNA oligomers to amino-coated paramagnetic beads is described. A hetero-bifunctional photo-reactive cross-linking chemical, 4-nitrophenyl 3-diazopyruvate, is applied to attach 5' amino-modified DNA to both silica and polystyrene paramagnetic beads. The coupling yields are comparable with similar methods in which no photo-reactive chemicals are used. The immobilised DNA on the polystyrene and silica beads was used efficiently in hybridisation experiments. An extension of this approach to light-directed immobilisation of specific DNA to beads, located at different positions in micro-flow reactors, opens up a range of integrated applications to complex diagnostics, evolutionary biotechnology and novel areas such as DNA computing.
Penchovsky
R
R
Biomolecular Information Processing (BioMIP), GMD-National Research Centre for Information Technology, Schloss Birlinghoven, 53754 Sankt Augustin, Germany. robert.penchovsky@gmd.de
Birch-Hirschfeld
E
E
McCaskill
J S
JS
D002851:Chromatography, High Pressure Liquid; D003432:Cross-Linking Reagents / Q000737:chemistry; D009596:Nitrophenols / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000235:genetics; D009841:Oligonucleotides / Q000737:chemistry* / Q000235:genetics; D010777:Photochemistry; D011137:Polystyrenes; D011773:Pyruvates / Q000737:chemistry*; D058428:Silica Gel; D012822:Silicon Dioxide
D003432:Cross-Linking Reagents; D009596:Nitrophenols; D015345:Oligonucleotide Probes; D009841:Oligonucleotides; D011137:Polystyrenes; D011773:Pyruvates; C061076:4-nitrophenyl 3-diazopyruvate; D058428:Silica Gel; D012822:Silicon Dioxide