title: Regional and age-related expression of gelatinases in the brains of young and old rats after treatment with kainic acid.
abstract: Due to the possible detrimental impact of local inflammatory responses in neurodegenerative disease, it was of interest to measure the expression of extracellular matrix-degrading enzymes, a group of proteases that are induced during an inflammatory response, in the brains of old and young animals in a model of neuronal death. Doses of kainic acid were administered that resulted in comparable hippocampal pyramidal neuron loss in young and old F344/BN hybrid rats, even though each age group received widely differing doses. Two matrix metalloproteinases (MMPs), MMP-2 and MMP-9, were differentially induced with respect to time after kainic acid in sensitive brain regions in both young and old rats. However, the elevation of MMP-9 in the temporal lobe 12 h after injection in old rats was significantly greater than that observed in young animals. These results suggest that early and late induction of MMPs may play a role in neuronal death and repair mechanisms, respectively, and that inflammatory mechanisms in the central nervous system (CNS) of old rats are exaggerated compared to young rats.
authors.affiliation: University of South Florida College of Medicine, Department of Pharmacology and Therapeutics, Tampa, FL 33612-4799, USA.
authors.forename: J W
authors.initials: JW
authors.lastname: Zhang
authors.forename: S
authors.initials: S
authors.lastname: Deb
authors.forename: P E
authors.initials: PE
authors.lastname: Gottschall
mesh_terms: D000367:Age Factors; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000201:enzymology; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology*; D007608:Kainic Acid / Q000494:pharmacology*; D020778:Matrix Metalloproteinase 2 / Q000187:drug effects* / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000187:drug effects* / Q000378:metabolism; D017966:Pyramidal Cells / Q000187:drug effects* / Q000201:enzymology; D051381:Rats; D011916:Rats, Inbred F344; D012640:Seizures / Q000139:chemically induced
chemical_list: D018690:Excitatory Amino Acid Agonists; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D007608:Kainic Acid