title: Correlation between profile of circulating mononuclear cells and clinical manifestations in patients with pemphigus vulgaris.
abstract: Phenotypes of 38 samples of mononuclear (PBMC) cells from 11 different patients with pemphigus vulgaris (PV) at different stages of the disease were explored looking for a possible relationship between cell immunity, mucocutaneous or mucosal lesion intensity and capacity of serum autoantibodies to elicit the disease in mice. PBMC from 5 patients with mucocutaneous lesions and sera with IgG capable of inducing the disease in neonatal mice had a high proportion of mature monocytes with CD14low DRhigh, and co-expressing CD16 and CD11b. In addition, a high proportion of CD19+CD5+ activated B cells and a very low proportion of naive CD4+CD45RA+ and CD8+CD11b+ T lymphocytes was observed. Monocytes from these patients expressed inducible nitric oxide synthase (iNOS). In contrast, PBMC from 6 patients, with lesions restricted to mucosal membranes and IgG lacking the capacity to induce the disease in mice, contained a high proportion of CD14high DRlow co-expressing CD16 circulating macrophages, CD8+CD11b+ T cells, and a low proportion of activated B lymphocytes. The results suggest a possible association between proportion of different antigen presenting cells (monocytes with high HLA-DR and low CD14 expression and activated B lymphocytes, or differentiated monocytes/macrophages), type of PV and capacity of serum autoantibodies to elicit the disease in mice.
authors.affiliation: Department of Biochemistry, University of Navarra, Pamplona, Spain.
authors.forename: A
authors.initials: A
authors.lastname: González
authors.forename: A
authors.initials: A
authors.lastname: España
authors.forename: M J
authors.initials: MJ
authors.lastname: López-Zabalza
authors.forename: B
authors.initials: B
authors.lastname: Pelacho
authors.forename: I
authors.initials: I
authors.lastname: Sánchez-Carpintero
authors.forename: E
authors.initials: E
authors.lastname: Santiago
authors.forename: N
authors.initials: N
authors.lastname: López-Moratalla
mesh_terms: D000051:Acantholysis / Q000209:etiology / Q000276:immunology / Q000473:pathology; D000818:Animals; D000831:Animals, Newborn; D001323:Autoantibodies / Q000008:administration & dosage / Q000097:blood; D004195:Disease Models, Animal; D006684:HLA-DR Antigens / Q000097:blood; D006801:Humans; D007116:Immunization, Passive; D007074:Immunoglobulin G / Q000008:administration & dosage / Q000097:blood; D016130:Immunophenotyping; D007963:Leukocytes, Mononuclear / Q000201:enzymology / Q000276:immunology*; D018950:Lipopolysaccharide Receptors / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000145:classification / Q000201:enzymology / Q000276:immunology; D019001:Nitric Oxide Synthase / Q000097:blood; D052247:Nitric Oxide Synthase Type II; D010392:Pemphigus / Q000201:enzymology / Q000276:immunology* / Q000473:pathology
chemical_list: D001323:Autoantibodies; D006684:HLA-DR Antigens; D007074:Immunoglobulin G; D018950:Lipopolysaccharide Receptors; C496319:NOS2 protein, human; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse