title: T-cell line adaptation of human immunodeficiency virus type 1 strain SF162: effects on envelope, vpu and macrophage-tropism.
abstract: Changes in co-receptor-use by human immunodeficiency virus type 1 (HIV-1) strains are relatively rare in vivo. Here we describe two variants derived from the CCR5-using strain SF162, selected for replication in the C8166 T-cell line. Amino acid substitutions in the V3 loop conferred CXCR4-use; however, the loss of macrophage-tropism by one variant was due to a single mutation in the start codon of vpu. We discuss how V3 loop and vpu mutations acquired by replication in T-cell lines in vitro correlate with similar changes reported for primary isolates and HIV-1 sequences in vivo.
authors.affiliation: The Wohl Virion Centre, Department of Immunology and Molecular Pathology, The Windeyer Institute of Medical Sciences, University College London, 46 Cleveland Street, London W1P 6DB, UK1.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Dejucq
authors.affiliation: The Wohl Virion Centre, Department of Immunology and Molecular Pathology, The Windeyer Institute of Medical Sciences, University College London, 46 Cleveland Street, London W1P 6DB, UK1.
authors.forename: Graham
authors.initials: G
authors.lastname: Simmons
authors.affiliation: The Wohl Virion Centre, Department of Immunology and Molecular Pathology, The Windeyer Institute of Medical Sciences, University College London, 46 Cleveland Street, London W1P 6DB, UK1.
authors.forename: Paul R
authors.initials: PR
authors.lastname: Clapham
mesh_terms: D000222:Adaptation, Physiological; D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D018387:Codon, Initiator / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics; D006678:HIV / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D019302:HIV Envelope Protein gp160 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D054298:Human Immunodeficiency Virus Proteins; D006801:Humans; D008264:Macrophages / Q000378:metabolism / Q000821:virology*; D008969:Molecular Sequence Data; D019713:Receptors, CCR5 / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000378:metabolism; D016415:Sequence Alignment; D012692:Serial Passage; D013601:T-Lymphocytes / Q000378:metabolism / Q000821:virology*; D054334:Viral Regulatory and Accessory Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D014779:Virus Replication; D000086082:Viroporin Proteins
chemical_list: D018387:Codon, Initiator; D004274:DNA, Recombinant; D004279:DNA, Viral; D019302:HIV Envelope Protein gp160; D054298:Human Immunodeficiency Virus Proteins; D019713:Receptors, CCR5; D019718:Receptors, CXCR4; D054334:Viral Regulatory and Accessory Proteins; C514859:vpu protein, Human immunodeficiency virus 1; D000086082:Viroporin Proteins