title: AaIT: from neurotoxin to insecticide.
abstract: AaIT is a single chain neurotoxic polypeptide derived from the venom of the Buthid scorpion Androctonus australis Hector, composed of 70 amino acids cross-linked by four disulfide bridges. Its strict selectivity for insects has been documented by toxicity, electrophysiological and ligand receptor binding assays. These last have shown that various insect neuronal membranes possess a single class of non-interacting AaIT binding sites of high affinity (K(D) = 1-3(n)M) and low capacity (0.5-2.0 pmol/mg prot.). The fast excitatory paralysis induced by AaIT is a result of a presynaptic effect, namely the induction of a repetitive firing in the terminal branches of the insect's motor nerves resulting in a massive and uncoordinated stimulation of the respective skeletal muscles. The neuronal repetitive activity is attributed to an exclusive and specific perturbation of sodium conductance as a consequence of toxin binding to external loops of the insect voltage-dependent sodium channel and modification of its gating mechanism. From a strictly agrotechnical point of view AaIT involvement in plant protection has taken the following two complementary forms: firstly, as a factor for the genetic engineering of insect infective baculoviruses resulting in potent and selective bio-insecticides. The efficacy of the AaIT-expressing, recombinant baculovirus is attributed mainly to its ability to continuously provide and translocate the gene of the expressed toxin to the insect central nervous system; secondly, based on the pharmacological flexibility of the voltage-gated sodium channel, as a device for insecticide resistance management. Channel mutations conferring resistance to a given class of insecticidal agents (such as the KDR phenomenon) may greatly increase susceptibility to the AaIT expressing bioinsecticides. Thus the AaIT is a pharmacological tool for the study of insect neuronal excitability and chemical ecology and the development of new approaches to insect control.
authors.affiliation: Department of Animal and Cell Biology, Institute of Life Sciences, The Hebrew University of Jerusalem, 91904, Jerusalem, Israel. zlotkin@vms.huji.ac.il
authors.forename: E
authors.initials: E
authors.lastname: Zlotkin
authors.forename: Y
authors.initials: Y
authors.lastname: Fishman
authors.forename: M
authors.initials: M
authors.lastname: Elazar
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D007305:Insecticide Resistance; D007306:Insecticides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D015640:Ion Channel Gating; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D012604:Scorpion Venoms / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D015222:Sodium Channels / Q000187:drug effects
chemical_list: D007306:Insecticides; D009498:Neurotoxins; D012604:Scorpion Venoms; D015222:Sodium Channels; C065634:AaIT neurotoxin, Androctonus australis