Cross-linking of LFA-1 induces secretion of macrophage inflammatory protein (MIP)-lalpha and MIP-1beta with consequent directed migration of activated lymphocytes.
Cross-linking of LFA-1 induces an active locomotory phenotype in T cells. In this study we demonstrate that cross-linking of LFA-1 using a monoclonal antibody results in the secretion of MIP-1alpha and MIP-1beta. Similar results were seen with anti-CD44 but not with anti-transferrin receptor or anti-MHC class 1. We examined the ability of activated lymphocytes to migrate onto a substrate consisting of large protein G-Sepharose beads coated with anti-LFA-1 and anti-CD44. In this system a signal is provided by cells at the point of contact with the beads. Cells migrated to cover the bead surface within 24 h. This contact was shown to be inhibited by the introduction of neutralizing antibodies to MIP-1alpha and MIP-1beta. Hence cross-linking of LFA-1 or CD44 induce chemokine secretion which may be of relevance in directional migration of lymphocytes.
Murphy
A
A
Department of Clinical Medicine, Trinity College Dublin, Ireland.
Long
A
A
Volkov
Y
Y
Kelleher
D
D
D000911:Antibodies, Monoclonal / Q000276:immunology; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D002634:Chemotaxis, Leukocyte; D003599:Cytoskeleton / Q000378:metabolism; D006680:HLA Antigens / Q000502:physiology; D006801:Humans; D018960:Hyaluronan Receptors / Q000502:physiology; D007527:Isoenzymes / Q000378:metabolism; D008213:Lymphocyte Activation; D016169:Lymphocyte Function-Associated Antigen-1 / Q000276:immunology / Q000502:physiology*; D019402:Macrophage Inflammatory Proteins / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism*; D008863:Microspheres; D016853:Muromonab-CD3 / Q000494:pharmacology; D010641:Phenotype; D011493:Protein Kinase C / Q000378:metabolism; D011940:Receptor Aggregation; D011990:Receptors, Transferrin / Q000502:physiology; D015398:Signal Transduction; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D006680:HLA Antigens; D018960:Hyaluronan Receptors; D007527:Isoenzymes; D016169:Lymphocyte Function-Associated Antigen-1; D019402:Macrophage Inflammatory Proteins; D016853:Muromonab-CD3; D011990:Receptors, Transferrin; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate