Similarity of bacteriocin activity profiles of mutans streptococci within the family when the children acquire the strains after the age of 5.
It has been shown that there is a window of infectivity for mutans streptococci between the ages of 19 and 31 months, when many children acquire mutans streptococci transmitted from their mothers. Part of the children that escape this window acquire mutans streptococci at a later age. In this group, maternal transmission is expected to be less prevalent. The present study compared the bacteriocin activity profiles of mutans streptococci isolated from mothers, fathers and children when the children acquired the mutans streptococci between the ages of 5 and 11. Twelve families were randomly selected from a group of 11-year-old children who were known to have acquired mutans streptococci during this age period. From the saliva of the mothers (n = 12), fathers (n = 8) and children (n = 12) approximately 30 mutans streptococci strains were isolated. All isolates were tested twice for bacteriocin activity against 21 indicator strains with a double-layer technique. Bacteriocin activity of strains was considered to be different when the number of strains against which bacteriocin was produced differed >1 or when the width of one or more inhibition zones differed > or =4 mm. In 7/12 mother-child pairs similar profiles were found. In the 8 father-child pairs similar profiles were only found on 2 occasions. In these 2 families, all 3 ( mother, father and child) harboured strains with a similar profile. In 4/8 father-mother pairs similar profiles were found. There was no correlation between the prevalence of mutans streptococci strains, the number of indicator strains against which the strains made bacteriocin, nor the mean size of the inhibition zones and the presence of similarity of bacteriocin activity profiles of mutans streptococci within the family members. The results show that even when a child acquires mutans streptococci after the age of 5, there may be similarity between mutans streptococci in mother, father and child, indicating that transmission between the family members occurs.
van Loveren
C
C
Department of Cariology Endodontology Pedodontology, Academic Centre for Dentistry Amsterdam (ACTA), Amsterdam, The Netherlands. C.van.Loveren@acta.nl
Buijs
J F
JF
ten Cate
J M
JM
D000367:Age Factors; D001430:Bacteriocins / Q000145:classification*; D002648:Child; D002675:Child, Preschool; D005192:Family Health; D005220:Fathers; D005260:Female; D006801:Humans; D008297:Male; D009035:Mothers; D015995:Prevalence; D012463:Saliva / Q000382:microbiology*; D013291:Streptococcus / Q000145:classification; D013295:Streptococcus mutans / Q000145:classification*; D019533:Streptococcus oralis / Q000145:classification; D013298:Streptococcus sanguis / Q000145:classification; D016986:Streptococcus sobrinus / Q000145:classification
D001430:Bacteriocins