Molecular analysis of a serotype 8 human astrovirus genome.
Human astroviruses are an important cause of gastroenteritis. As part of a molecular epidemiological study carried out in Mexico a human astrovirus isolate, Yuc-8, was adapted to grow in CaCo-2 cells, and its entire genome was sequenced. A 15 amino acid deletion in ORF1a, which has been associated with adaptation of astroviruses to grow in cells other than CaCo-2, was present in Yuc-8. Comparative sequence analysis of the Yuc-8 ORF2 with reported human astrovirus sequences revealed that this isolate belongs to genotype (serotype) 8. Two distinct domains in ORF2 were observed: an amino-terminal domain (residues 1 to 415), with identities higher than 81% among the strains analysed, and a carboxy-terminal domain (residues 416 to 782) with identities between 36 and 60%. Two non-superimposable phylogenetic trees were generated by separate analysis of these two domains, suggesting that a differential selective pressure is exerted along the structural polyprotein.
Méndez-Toss
Martha
M
Departamento de Genética y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apartado Postal 510-3, Colonia Miraval, Cuernavaca, Morelos 62250, Mexico1.
Romero-Guido
Pedro
P
Departamento de Genética y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apartado Postal 510-3, Colonia Miraval, Cuernavaca, Morelos 62250, Mexico1.
Munguía
Maria Elena
ME
Departamento de Genética y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apartado Postal 510-3, Colonia Miraval, Cuernavaca, Morelos 62250, Mexico1.
Méndez
Ernesto
E
Departamento de Genética y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apartado Postal 510-3, Colonia Miraval, Cuernavaca, Morelos 62250, Mexico1.
Arias
Carlos F
CF
Departamento de Genética y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apartado Postal 510-3, Colonia Miraval, Cuernavaca, Morelos 62250, Mexico1.
D000595:Amino Acid Sequence; D018938:Caco-2 Cells; D016679:Genome, Viral; D005838:Genotype; D006801:Humans; D001257:Mamastrovirus / Q000737:chemistry* / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D008800:Mexico; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D020815:Polyproteins / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D017384:Sequence Deletion / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012703:Serotyping; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
D020815:Polyproteins; D014764:Viral Proteins