PMA-treated K-562 leukemia cells mediate a TH2-specific expansion of CD4+ T cells in vitro.
Highly enriched preparations of human CD3+CD4+ T-lymphocytes were stimulated with mitogen or OKT3 to determine the capacity of K-562 cells to function as accessory cells. Phorbol 12-myristate 13-acetate (PMA)-treated K-562 cells were induced to differentiate along the megakaryocytic lineage and could supplant monocyte-accessory cell function. Intracytoplasmic analysis of interleukin-4 (IL-4) and interferon-gamma (IFN-gamma) established that IL-4, and not IFN-gamma, was preferentially produced by the activated lymphocytes. This polarized stimulation is compatible with a type 2 or humoral immune response of purified T cells co-cultured with differentiated K-562 cells in vitro, and may have implications in immunoregulation due to disease progression.
Baker
E J
EJ
Department of Medical Biology, Graduate School of Medicine, University of Tennessee Medical Center at Knoxville, 1924 Alcoa Highway, Knoxville, TN 37920, USA.
Ichiki
A T
AT
Hodge
J W
JW
Sugantharaj
D
D
Bamberger
E G
EG
Lozzio
C B
CB
D017252:CD3 Complex; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology*; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D020014:K562 Cells / Q000276:immunology*; D008213:Lymphocyte Activation; D008934:Mitogens; D013755:Tetradecanoylphorbol Acetate; D018417:Th1 Cells; D018418:Th2 Cells / Q000502:physiology*
D017252:CD3 Complex; D008934:Mitogens; D013755:Tetradecanoylphorbol Acetate