CD95-mediated apoptosis and DNA fragmentation in MS.
OBJECTIVE
To investigate possible associations of soluble CD95 (sCD95) serum levels and DNA defragmentation with different MS disease stages and activities.


METHODS
Sera of 114 patients were analysed by an ELISA technique for sCD95. In a subgroup of 18 relapsing-remitting MS patients and controls we studied DNA fragmentation by the TUNEL-method in CSF cytospins.


RESULTS
sCD95 was detectable in sera of MS patients, healthy controls and meningitis patients without significant differences. CSF specimens showed modest amounts of apoptotic cells in MS and controls.


CONCLUSIONS
We could not demonstrate an association of MS disease course or activity with the expression of sCD95 in sera. DNA fragmentation in the CSF was not significantly enhanced compared to controls. Thus the analysed markers of programmed cell death appear not suitable to monitor MS disease courses.
Heesen
C
C
Department of Neurology, University of Hamburg, Germany. heesen@uke.uni-hamburg.de
Georghiu
S
S
Gbadamosi
J
J
Schoser
B G
BG
D000328:Adult; D017209:Apoptosis; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D053938:DNA Fragmentation / Q000235:genetics; D005260:Female; D006801:Humans; D020287:In Situ Nick-End Labeling; D008297:Male; D008587:Meningitis, Viral / Q000235:genetics / Q000276:immunology; D008875:Middle Aged; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000134:cerebrospinal fluid / Q000235:genetics* / Q000276:immunology*; D011237:Predictive Value of Tests; D019014:fas Receptor / Q000097:blood* / Q000235:genetics
D015415:Biomarkers; D019014:fas Receptor