Hydrogen peroxide activates the SoxRS regulon in vivo.
By multiplex reverse transcription-PCR, we demonstrate that the SoxRS response, which protects cells against superoxide toxicity, is triggered also by hydrogen peroxide. SoxR-dependent inductions of 7. 3-, 7.6-, 4.6-, 2.2-, and 2.6-fold were quantified for soxS, micF, sodA, inaA, and fpr transcripts, respectively. This finding suggests an extensive and tight connectivity between different regulatory pathways in the Escherichia coli response to oxidative stress.
Manchado
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M
Departamento de Bioquímica y Biología Molecular, Universidad de Córdoba, 14071 Córdoba, Spain.
Michán
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C
Pueyo
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C
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000378:metabolism* / Q000494:pharmacology; D018085:Regulon; D013482:Superoxide Dismutase / Q000235:genetics; D015534:Trans-Activators; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C070724:SoxR protein, Bacteria; C070725:SoxS protein, E coli; D006861:Hydrogen Peroxide; C095500:SodA protein, Bacteria; D013482:Superoxide Dismutase