Use of the indirect immunofluorescence method for detection and enumeration of Escherichia coli in seawater samples.
The determination of Escherichia coli in marine waters through a rapid method, the microscopic indirect immunofluorescent technique, is evaluated in comparison with the conventional count on m-FC agar medium. The data obtained in seawater samples, collected monthly along the Messina coastline, show good sensitivity of the analysis and agreement between the microscopic and culture technique, with a detection limit of 10(2) cells 100 ml(-1) for immunofluorescence.
Caruso
G
G
Istituto Sperimentale Talassografico-Consiglio Nazionale Ricerche, Messina, Italy.
Zaccone
R
R
Crisafi
E
E
D015169:Colony Count, Microbial / Q000379:methods*; D003470:Culture Media; D004926:Escherichia coli / Q000302:isolation & purification*; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods*; D012623:Seawater / Q000382:microbiology*; D012680:Sensitivity and Specificity
D003470:Culture Media