title: Expression of c-jun in the lateral vestibular nucleus following spinal cord injury and peripheral nerve graft transplantation in adult rats.
abstract: The time course of c-jun expression and the effect of a peripheral nerve (PN) graft on axonal regeneration and c-jun expression in the lateral vestibular nucleus (LVN) were investigated in this study. c-Jun was found in LVN neurons 1 day after hemisection of the spinal cord at C3, with weak immunoreactivity. Strong c-Jun positive immunoreactivity was found 3 days post-injury. The expression of c-jun reached its maximum level (45% +/- 2.5, mean +/- SD) 1 week post-injury and gradually decreased to about a third of its peak value 6 weeks after the inflicted lesion. The PN graft implanted at the lesion site significantly enhanced the number of neurons expressing c-jun 4 weeks after PN transplantation (from 20% of LVN neurons in axotomy alone to 30% in PN graft transplantation, P < 0. 05). The PN graft induced injured neurons to regrow into the PN graft. About 91 FG-labeled neurons were found in the LVN and approximately 87% of these neurons showed c-Jun positive immunoreactivity. The high percentage of regenerating neurons expressing c-jun suggested that c-Jun might be involved in the regenerative process. The result of double staining for c-Jun and NOS showed that approximately 93% NOS positive neurons co-expressed c-Jun. Comparing the time course of the appearance of c-Jun with that of NOS, the expression of c-jun preceded that of NOS. Although a high percentage of NOS reactive neurons expressed c-jun, the expression of NOS in damaged LVN neurons needs to be further investigated.
authors.affiliation: Department of Anatomy, Faculty of Medicine, The University of Hong Kong, Hong Kong, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Jin
authors.forename: D
authors.initials: D
authors.lastname: Tay
authors.forename: K F
authors.initials: KF
authors.lastname: So
authors.forename: W
authors.initials: W
authors.lastname: Wu
mesh_terms: D000367:Age Factors; D000818:Animals; D005260:Female; D009416:Nerve Regeneration / Q000502:physiology; D009474:Neurons / Q000737:chemistry / Q000201:enzymology; D019001:Nitric Oxide Synthase / Q000032:analysis / Q000096:biosynthesis; D016755:Proto-Oncogene Proteins c-jun / Q000032:analysis / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000378:metabolism* / Q000601:surgery*; D013979:Tibial Nerve / Q000637:transplantation*; D003689:Vestibular Nucleus, Lateral / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*
chemical_list: D016755:Proto-Oncogene Proteins c-jun; D019001:Nitric Oxide Synthase