Genetic relationships among biotypes of Bemisia tabaci (Hemiptera: Aleyrodidae) based on AFLP analysis.
Genetic similarities between 13 samples belonging to nine reference biotypes and two field populations of Bemisia tabaci (Gennadius), one field population of B. medinae Gómez-Menor and another of B. afer Priesner & Hosny, were evaluated using amplified fragment length polymorphism (AFLP) markers. The results indicate that B. tabaci biotypes can be grouped together with a minimum similarity coefficient of 0.32 and separated from the two other species with a similarity coefficient of 0.07. Bemisia tabaci biotypes were grouped in four clusters which comprised: (i) Near East and Indian subcontinent biotypes; (ii) B and Q biotypes plus a Nigerian population from cowpea; (iii) New World A biotype; and (iv) S biotype and a Nigerian population from cassava. These results were consistent with a previous grouping of biotypes based on RAPD-PCR analysis. The AFLP assay allowed the scoring of a total of 354 polymorphic bands in two reaction events with the use of two primer combinations.
Cervera
M T
MT
CNB-CSIC, Campus de Cantoblanco, Madrid, 28049, Spain.
Cabezas
J A
JA
Simón
B
B
Martínez-Zapater
J M
JM
Beitia
F
F
Cenis
J L
JL
D000818:Animals; D005784:Gene Amplification; D017344:Genes, Insect; D006430:Hemiptera / Q000145:classification* / Q000235:genetics; D011110:Polymorphism, Genetic