The dipyridamole effect on the photoactive bacteriochlorophyll interaction with quinone acceptors in reaction centers of purple bacteria.
The effect of Dipyridamole (10(-6)-10(-3) M) on the photomobilized electron transport in the system of quinone acceptors Q(A)-Q(B) of isolated photosynthetic reaction centers of Rhodobacter sphaeroides and on its temporary stabilization on Q(B) was studied. Depending on the type of the detergent present in the reaction center (lauryl dimethylamine oxide, Triton X-100, sodium dodecyl sulfate, and sodium cholate), dipyridamole could increase the time of the electron transfer to Q(B). The dipyridamole effect on the efficiency of the electron stabilization on Q(B) for reaction centers with different detergents was revealed in slowing down the process of dark reduction of photoactive bacteriochlorophyll from Q(B) at initial concentrations of added dipyridamole (10(-6)-10(-5) M) with following acceleration of the process at the dipyridamole concentrations of 10(-4)-10(-3) M. The pH lowering from 6.8-7.0 to 5.9-6.0 increased the dipyridamole effect. The possibility of the dipyridamole effect on the structural-dynamic state of the reaction center complex, including its hydrogen bond system, which influences the studied parameters of functional activity, is suggested.
Churbanova IYu
Biological Department, Lomonosov Moscow State University, Russia.
Knox
P P
PP
Lukashev
E P
EP
Borissevitch
G P
GP
Zakharova
N I
NI
D001429:Bacteriochlorophylls / Q000737:chemistry*; D016227:Benzoquinones / Q000737:chemistry*; D004176:Dipyridamole / Q000494:pharmacology*; D045342:Light-Harvesting Protein Complexes; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry*; D012242:Rhodobacter sphaeroides / Q000737:chemistry / Q000502:physiology; D013329:Structure-Activity Relationship
D001429:Bacteriochlorophylls; D016227:Benzoquinones; D045342:Light-Harvesting Protein Complexes; D010726:Phosphodiesterase Inhibitors; D045322:Photosynthetic Reaction Center Complex Proteins; C004532:quinone; D004176:Dipyridamole