Cellular and sub-cellular localisation of GABA(B1) and GABA(B2) receptor proteins in the rat cerebellum.
Following the recent discovery that GABA(B) receptors expressed in cell lines are only functional when both GABA(B1) and GABA(B2) are expressed, the present study reports on the development of polyclonal antisera specific for carboxyl-terminal portions of the two related GABA(B) receptor components respectively. Western blotting indicated the specificity of affinity-purified antibodies for native or recombinant expressed GABA(BR1) and GABA(BR2), with no cross-reactivity, both antisera detecting the heterodimer in rat cerebellar membranes. Immunohistochemistry revealed a distinct distribution of both receptor proteins in rat cerebellum. GABA(B1) immunoreactivity was primarily located in the granule cell layer and Purkinje cells, with discrete immuno-positive cell bodies being present in the molecular layer. GABA(B2) staining revealed intense immunoreactivity in the molecular layer, with weaker staining in the granule cell layer. Purkinje cell bodies were less intensely immuno-positive for GABA(B2). Co-localisation of both receptor proteins was observed using double immunofluorescence techniques, consistent with the notion that both proteins are required for the formation of functional GABA(B) receptors in vivo. Immunofluorescence also indicated that GABA(B) receptors did not co-localise with glial fibrillary acid protein, confirming a neuronal localisation for GABA(B) receptors. Electron microscopic analysis of the molecular layer revealed that the distribution of immunolabelling for both GABA(B1) and GABA(B2) was mainly located on the membrane of Purkinje cell dendrites and spines and in parallel fibre terminals.
Ige
A O
AO
Department of Neurobiology, Babraham Institute, Babraham, CB2 4AT, Cambridge, UK.
Bolam
J P
JP
Billinton
A
A
White
J H
JH
Marshall
F H
FH
Emson
P C
PC
D000818:Animals; D000918:Antibody Specificity; D015153:Blotting, Western; D002531:Cerebellum / Q000737:chemistry* / Q000166:cytology; D003429:Cross Reactions; D007150:Immunohistochemistry; D008297:Male; D016253:Microscopy, Immunoelectron; D011689:Purkinje Cells / Q000737:chemistry* / Q000648:ultrastructure; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D018079:Receptors, GABA / Q000032:analysis / Q000276:immunology; D018080:Receptors, GABA-B / Q000032:analysis* / Q000276:immunology; D012756:Sheep; D016610:Tissue Embedding
C116566:Gabbr2 protein, rat; D018079:Receptors, GABA; D018080:Receptors, GABA-B