Expression of calcium-binding protein S100A2 in breast lesions.
A suppression subtraction cDNA library representing mRNAs expressed at a higher level in a benign breast tumour-derived cell line relative to the malignant MCF-7A cell line contained cDNAs corresponding to mRNAs for plasminogen activator inhibitor I, annexin VIII and the EF-hand protein S100A2. S100A2 protein has previously been shown to be expressed in normal human breast epithelium, but not in human breast carcinoma cell lines. Using a PCR-based assay and in situ hybridization on histological sections of human breast specimens, the mRNA for S100A2 was shown to be present in all benign breast lesions examined as well as in normal epithelium. S100A2 mRNA was detectable in 37% of specimens of carcinoma in situ, but in less than 15% of carcinoma specimens. The results suggest that the loss of S100A2 is associated with the development of malignant cells and is not associated with early tumour development.
Liu
D
D
School of Biological Sciences, University of Liverpool, P.O. Box 147, Liverpool, L69 7ZB, UK.
Rudland
P S
PS
Sibson
D R
DR
Platt-Higgins
A
A
Barraclough
R
R
D015152:Blotting, Northern; D001940:Breast / Q000378:metabolism / Q000502:physiology; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism*; D002278:Carcinoma in Situ / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D002630:Chemotactic Factors / Q000096:biosynthesis* / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D004847:Epithelial Cells / Q000378:metabolism / Q000502:physiology; D006801:Humans; D017403:In Situ Hybridization; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D009418:S100 Proteins / Q000096:biosynthesis* / Q000235:genetics; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis
D002630:Chemotactic Factors; D018076:DNA, Complementary; D004273:DNA, Neoplasm; D012333:RNA, Messenger; D009418:S100 Proteins; C098997:S100A2 protein, human; D016159:Tumor Suppressor Protein p53