title: Platelet monoamine oxidase B activity is inversely associated with plasma cotinine concentration.
abstract: Cigarette smoke but not nicotine inhibits irreversibly platelet monoamine oxidase B (MAO-B) activity. Current smokers have decreased platelet MAO-B activity which normalizes when smokers quit. Normalization of platelet MAO-B activity probably depends on platelet turnover. Platelet MAO-B activity has been found to be correlated with plasma thiocyanate concentration. The aim of this study was to investigate in smokers whether platelet MAO-B activity is related to plasma cotinine concentration, an indirect index of use of smoked tobacco. We determined simultaneously plasma cotinine concentration and platelet MAO-B activity in 85 cigarette smokers [mean (+/- SD) age 39 +/- 8.8 years, 55 men]. Platelet MAO-B activity was lower in male than in female smokers (14.1 +/- 7.9 versus 17.8 +/- 7.2 nmol/h/10(9) platelets, p = 0.03). Platelet MAO-B activity correlated positively with age (r = 0.26, p = 0.01) and inversely with plasma cotinine concentration (r = -0.32, p = 0.002) but not with number of cigarettes smoked or with Fagerström Tolerance Questionnaire score. Multiple linear regression analysis showed that 49% of the age-adjusted variance in platelet MAO-B activity (R2 = 0.489, p < 0.0001) was explained by plasma cotinine concentration (p < 0.001) and gender (p = 0.037). It was concluded that platelet MAO-B activity in smokers is inversely associated with plasma cotinine level, an index of smoked tobacco use. Further studies are needed to investigate whether measurement of platelet MAO-B activity can be used as a long-term index of tobacco use and smoke exposure.
authors.affiliation: Department of Pharmacology, University Hospital Pitié-Salpêtrière, Paris, France. ivan.berlin@psl.ap-hop-paris.fr
authors.forename: I
authors.initials: I
authors.lastname: Berlin
authors.forename: O
authors.initials: O
authors.lastname: Spreux-Varoquaux
authors.forename: J M
authors.initials: JM
authors.lastname: Launay
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D001792:Blood Platelets / Q000201:enzymology*; D003367:Cotinine / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008995:Monoamine Oxidase / Q000378:metabolism*; D012044:Regression Analysis; D012737:Sex Factors; D012907:Smoking / Q000097:blood
chemical_list: D008995:Monoamine Oxidase; D003367:Cotinine