title: fbpABC gene cluster in Neisseria meningitidis is transcribed as an operon.
abstract: The neisserial fbpABC locus has been proposed to constitute a single transcriptional unit. To confirm this operonic arrangement, transcription assays using reverse transcriptase PCR amplification were conducted with Neisseria meningitidis. The presence of fbpAB and fbpBC transcripts obtained by priming cDNA synthesis with an fbpC-sequence-specific oligonucleotide indicates that fbpABC is organized as a single expression unit. The ratio of fbpA to fbpABC mRNA was approximately between 10- to 20-fold, as determined by real-time quantitative PCR.
authors.affiliation: Department of Microbiology and Infectious Disease, University of Calgary, Calgary, Alberta, Canada T2N 4N1.
authors.forename: H H
authors.initials: HH
authors.lastname: Khun
authors.forename: V
authors.initials: V
authors.lastname: Deved
authors.forename: H
authors.initials: H
authors.lastname: Wong
authors.forename: B C
authors.initials: BC
authors.lastname: Lee
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D005810:Multigene Family; D009345:Neisseria meningitidis / Q000235:genetics*; D009876:Operon; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; C119770:FlpA protein, bacteria; C119771:FlpB protein, Lactococcus lactis; D012333:RNA, Messenger; D014157:Transcription Factors