Cytoskeleton mediating transport between the ER system and the Golgi apparatus in the green alga Scenedesmus acutus.
In the green alga Scenedesmus acutus, Golgi bodies are located near the nucleus and supplied with transition vesicles that bud from the outer nuclear envelope membrane. Using this alga, we have shown previously that thiamine pyrophosphatase (TPPase), a marker enzyme of Golgi bodies, migrates in vesicles from the Golgi bodies to the ER via the nuclear envelope in the presence of BFA (Noguchi et al., Protoplasma 201, 202-212, 1998). In this study we demonstrate that both cytochalasin B and oryzalin (microtubule-disrupting agent) inhibit the BFA-induced migration of TPPase from Golgi bodies to the nuclear envelope. However, only actin filaments--not microtubules--can be detected between the nuclear envelope and the Golgi bodies in both BFA-treated and untreated cells. These observations suggest that actin filaments mediate the BFA-induced retrograde transport of vesicles. This mechanism differs from that found in mammalian cells, in which microtubules mediate BFA-induced retrograde transport by the elongation of membrane tubules from the Golgi cisternae. We also discuss the non-participation of the cytoskeleton in anterograde transport from the nuclear envelope to the Golgi bodies.
Tanaka
Y
Y
Department of Biological Science, Nara Women's University, Japan.
Noguchi
T
T
D000935:Antifungal Agents / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D020126:Brefeldin A / Q000494:pharmacology; D002467:Cell Nucleus / Q000378:metabolism; D000460:Chlorophyta / Q000378:metabolism* / Q000648:ultrastructure; D020285:Cryoelectron Microscopy; D003571:Cytochalasin B / Q000494:pharmacology; D003599:Cytoskeleton / Q000378:metabolism*; D004136:Dinitrobenzenes / Q000494:pharmacology; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism*; D006540:Herbicides / Q000494:pharmacology; D016253:Microscopy, Immunoelectron; D008870:Microtubules / Q000378:metabolism; D008954:Models, Biological; D013424:Sulfanilamides; D013834:Thiamine Pyrophosphatase / Q000378:metabolism
D000935:Antifungal Agents; D004136:Dinitrobenzenes; D006540:Herbicides; D013424:Sulfanilamides; D020126:Brefeldin A; D003571:Cytochalasin B; C012465:oryzalin; D013834:Thiamine Pyrophosphatase