The effect of zaldaride maleate, an antidiarrheal compound, on acetylcholine-induced intestinal electrolyte secretion.
The effect of zaldaride on acetylcholine-induced colonic electrolyte secretion was examined. The short-circuit current response to acetylcholine was partially reduced by tetrodotoxin, a neuronal blocker, and was completely inhibited by atropine, an acetylcholine M receptor antagonist, in the rat colonic preparations. The tetrodotoxin sensitive effect was significantly inhibited by zaldaride, whereas the tetrodotoxin insensitive effect was not affected. Acetylcholine release from synaptosomes of submucosal nerves of guinea-pig colon was significantly reduced by zaldaride. Zaldaride may reduce colonic electrolyte secretion by acetylcholine due to the inhibition of acetylcholine release from synaptosomes of colonic submucosal nerves.
Aikawa
N
N
The Drug Development Research Laboratories, Pharmaceutical Research Institute, Kyowa Hakko Kogyo Co., Ltd., 1188 Shimotogari, Nagaizumi-cho, Sunto-gun, Shizuoka, Japan. AIKAWA@kyowa.co.jp
Kishibayashi
N
N
Karasawa
A
A
Ohmori
K
K
D000109:Acetylcholine / Q000494:pharmacology*; D000779:Anesthetics, Local / Q000494:pharmacology; D000818:Animals; D000930:Antidiarrheals / Q000494:pharmacology*; D001562:Benzimidazoles / Q000494:pharmacology*; D003106:Colon / Q000187:drug effects / Q000294:innervation / Q000378:metabolism; D015194:Diffusion Chambers, Culture; D004573:Electrolytes / Q000378:metabolism*; D004594:Electrophysiology; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000187:drug effects; D008297:Male; D018727:Muscarinic Antagonists / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011976:Receptors, Muscarinic / Q000187:drug effects; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism; D013779:Tetrodotoxin / Q000494:pharmacology
D000779:Anesthetics, Local; D000930:Antidiarrheals; D001562:Benzimidazoles; D004573:Electrolytes; D018727:Muscarinic Antagonists; D011976:Receptors, Muscarinic; C052087:CGS 9343B; D013779:Tetrodotoxin; D000109:Acetylcholine