title: Viability and fertility of rabbit spermatozoa diluted in Tris-buffer extenders and stored at 15 degrees C.
abstract: Artificial insemination (AI) in rabbits is not extensive in the breeding programs of the rabbit meat industry. A limiting factor is related to the semen preservation. In order to improve the use of AI, two experiments have been conducted to evaluate sperm viability and fertility of rabbit semen chilled and stored at 15 degrees C after dilution in Tris-based extenders. In Experiment 1, pooled semen samples were diluted 1:10 (semen/extender) in four different Tris-based extenders (Tris-citric-glucose (TCG), TES-Tris-glucose (TTG), Tris-citric-fructose (TCF) and TES-Tris-fructose (TTF)) and stored at 15 degrees C. Sperm viability was evaluated at 0, 24, 48, 72 and 96 h following dilution for total sperm motility (TSM), forward progressive motility (FPM), plasma membrane integrity (PMI) and acrosome integrity (NAR). Viability of spermatozoa declined with time of storage (P<0.05), irrespective of the extender used. There were interactions between extender and time of storage (P<0.05) in all viability parameters evaluated. After 96 h of storage, TCG provided the highest sperm viability (P<0.05) and TTG the lowest (P>0.05). In Experiment 2, a field trial was conducted at a commercial farm to evaluate the conception and farrowing rates of rabbit spermatozoa extended in TCG. After synchronization of oestrous and induction of ovulation, 3713 does with different physiological conditions (nulliparous, primiparous, lactating and re-breeding) were inseminated one time (15x10(6) sperm per doses) with semen stored at 0 (n: 1275), 24 (n: 1503) and 48 h (n: 935) at 15 degrees C. Overall conception and farrowing rates were 77.1+/-0.7 and 70.4+/-0.7, respectively, and the mean litter size was 7.6+/-0.1. Fertility results were unaffected by the time of semen storage (P>0.05). Regardless of time of semen storage, fertility results were affected by the physiological conditions of does (P<0.05). Nulliparous and lactating does showed the highest fertility and primiparous the lowest. In summary, these results indicate that Tris-buffer extenders are effective for preserving viability and fertilizing capability of rabbit spermatozoa stored at 15 degrees C.
authors.affiliation: Department of Animal Pathology (Animal Reproduction), Veterinary Medicine, University of Murcia, Campus de Espinardo, 30.071, Murcia, Spain. roca@um.es
authors.forename: J
authors.initials: J
authors.lastname: Roca
authors.forename: S
authors.initials: S
authors.lastname: Martínez
authors.forename: J M
authors.initials: JM
authors.lastname: Vázquez
authors.forename: X
authors.initials: X
authors.lastname: Lucas
authors.forename: I
authors.initials: I
authors.lastname: Parrilla
authors.forename: E A
authors.initials: EA
authors.lastname: Martínez
mesh_terms: D020101:Acrosome Reaction / Q000502:physiology; D000818:Animals; D002064:Buserelin / Q000008:administration & dosage; D003080:Cold Temperature; D005260:Female; D005298:Fertility / Q000502:physiology*; D006064:Gonadotropins, Equine / Q000008:administration & dosage; D007315:Insemination, Artificial / Q000379:methods / Q000662:veterinary*; D016014:Linear Models; D008297:Male; D008856:Microscopy, Fluorescence / Q000662:veterinary; D008858:Microscopy, Phase-Contrast / Q000662:veterinary; D011817:Rabbits / Q000502:physiology*; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000502:physiology*; D018709:Statistics, Nonparametric
chemical_list: D006064:Gonadotropins, Equine; D002064:Buserelin