Cloning and expression of murine Hsp40 gene: differences in initiation sites between heat-induced and constitutive transcripts.
We have isolated cDNA and genomic clones of murine Hsp40 (mmDjB1). The murine Hsp40 gene was expressed at a high level in testis and was induced by heat shock. The transcriptional initiation sites were different between heat-induced transcripts and constitutive ones. The heat-induced transcripts initiated 22 to 28 bp downstream of the TATA box, whereas constitutive transcripts initiated from multiple sites, many of which initiated upstream of the TATA box. To our knowledge, this is the first report on the heat shock-dependent usage of transcriptional start sites of a heat shock gene.
Hata
M
M
Cell Stress Biology Research Group, Aichi Cancer Center Research Institute, Nagoya, Japan.
Ohtsuka
K
K
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005091:Exons; D005786:Gene Expression Regulation; D050956:HSP40 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics*; D006358:Hot Temperature; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D011994:Recombinant Proteins / Q000737:chemistry; D016385:TATA Box; D013737:Testis / Q000378:metabolism; D014158:Transcription, Genetic
C494641:Dnajb1 protein, mouse; D050956:HSP40 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D011994:Recombinant Proteins