An appraisal of telomerase activity in benign prostatic hyperplasia.
BACKGROUND
Prostate cancer is one of the commonest neoplasms in elderly males in developed countries. It is not clear which individuals are at high risk of developing aggressive adenocarcinoma of the prostate. Biomarkers are therefore urgently needed to identify such individuals. It had been suggested both by ourselves and others that prostatic telomerase activity may represent a valuable marker in this respect, particularly if applied to BPH, as tissue is readily available from both transurethral resection of prostates and transrectal ultrasound biopsy.


METHODS
Tissue was collected prospectively from 46 patients with BPH who underwent TURP for clinically benign prostatic disease, and who were examined using the telomeric repeat amplification protocol (TRAP assay).


RESULTS
Telomerase activity was not detected in any of 46 BPH samples, using TRAP assay conditions of 0.12, 1.2, and 12.0 microg protein.


CONCLUSIONS
The study confirms that telomerase is not detectable in BPH samples. This would suggest that absence of telomerase activity may be a strong indicator of a lack of cancer. However further studies are necessary to confirm this.
Caldarera
E
E
Department of Urology, King's College Hospital, London, UK. caldarera@hotmail.com
Crooks
N H
NH
Muir
G H
GH
Pavone-Macaluso
M
M
Carmichael
P L
PL
D000230:Adenocarcinoma / Q000209:etiology*; D000328:Adult; D014408:Biomarkers, Tumor / Q000032:analysis*; D006801:Humans; D008297:Male; D021141:Nucleic Acid Amplification Techniques; D011446:Prospective Studies; D011470:Prostatic Hyperplasia / Q000150:complications / Q000201:enzymology* / Q000601:surgery; D011471:Prostatic Neoplasms / Q000209:etiology*; D012307:Risk Factors; D019098:Telomerase / Q000378:metabolism*; D020728:Transurethral Resection of Prostate
D014408:Biomarkers, Tumor; D019098:Telomerase