title: Mutations in cartilage oligomeric matrix protein causing pseudoachondroplasia and multiple epiphyseal dysplasia affect binding of calcium and collagen I, II, and IX.
abstract: Mutations in type 3 repeats of cartilage oligomeric matrix protein (COMP) cause two skeletal dysplasias, pseudoachondroplasia (PSACH) and multiple epiphyseal dysplasia (MED). We expressed recombinant wild-type COMP that showed structural and functional properties identical to COMP isolated from cartilage. A fragment encompassing the eight type 3 repeats binds 14 calcium ions with moderate affinity and high cooperativity and presumably forms one large disulfide-bonded folding unit. A recombinant PSACH mutant COMP in which Asp-469 was deleted (D469 Delta) and a MED mutant COMP in which Asp-361 was substituted by Tyr (D361Y) were both secreted into the cell culture medium of human cells. Circular dichroism spectroscopy revealed only small changes in the secondary structures of D469 Delta and D361Y, demonstrating that the mutations do not dramatically affect the folding and stability of COMP. However, the local conformations of the type 3 repeats were disturbed, and the number of bound calcium ions was reduced to 10 and 8, respectively. In addition to collagen I and II, collagen IX also binds to COMP with high affinity. The PSACH and MED mutations reduce the binding to collagens I, II, and IX and result in an altered zinc dependence. These interactions may contribute to the development of the patient phenotypes and may explain why MED can also be caused by mutations in collagen IX genes.
authors.affiliation: Institute for Biochemistry, Medical Faculty, University of Cologne, D-50931 Köln, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Thur
authors.forename: K
authors.initials: K
authors.lastname: Rosenberg
authors.forename: D P
authors.initials: DP
authors.lastname: Nitsche
authors.forename: T
authors.initials: T
authors.lastname: Pihlajamaa
authors.forename: L
authors.initials: L
authors.lastname: Ala-Kokko
authors.forename: D
authors.initials: D
authors.lastname: Heinegård
authors.forename: M
authors.initials: M
authors.lastname: Paulsson
authors.forename: P
authors.initials: P
authors.lastname: Maurer
mesh_terms: D000130:Achondroplasia / Q000209:etiology / Q000235:genetics*; D002118:Calcium / Q000378:metabolism*; D064236:Cartilage Oligomeric Matrix Protein; D002460:Cell Line; D003094:Collagen / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D064235:Matrilin Proteins; D009154:Mutation; D010009:Osteochondrodysplasias / Q000209:etiology / Q000235:genetics*; D011487:Protein Conformation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D020449:Repetitive Sequences, Amino Acid
chemical_list: D064236:Cartilage Oligomeric Matrix Protein; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D064235:Matrilin Proteins; C577553:TSP5 protein, human; D003094:Collagen; D002118:Calcium