Fluorescent labelling of oligodeoxyribonucleotides by the oxyamino-aldehyde coupling reaction.
We describe the reaction of oligonucleotides containing an aldehydic group at the 5'-end or inside the sequence with an oxyamino label. The reaction was found to be highly selective and represents an efficient method for derivatization of oligonucleotides.
Trévisiol
E
E
Chimie BioOrganique, LEDSS, UMR 5616, Université Joseph Fourier, Grenoble, France.
Renard
A
A
Defrancq
E
E
Lhomme
J
J
D000447:Aldehydes / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013696:Temperature
D000447:Aldehydes; D005456:Fluorescent Dyes; D009838:Oligodeoxyribonucleotides; D004247:DNA