Riboflavin transport by isolated perfused rabbit renal proximal tubules.
Rabbit renal proximal tubular transport of riboflavin (RF) was examined by using the in vitro isolated tubule perfusion technique. We found that proximal tubules actively reabsorbed (J(lb)) and secreted (J(bl)) RF. At 0.1 microM RF concentration, J(bl) was significantly higher than J(lb), resulting in a net secretion. This net secretion of RF was decreased at 0.01 microM RF concentration and increased at 1 microM RF concentration. Both J(lb) and J(bl) were inhibited by lowering temperature or by adding iodoacetate, a metabolic inhibitor, and lumichrome, an RF analog, suggesting the involvement of carrier-mediated transport mechanisms. J(bl) was inhibited by probenecid, an anion transport inhibitor, and by para-aminohippuric acid, an organic anion, suggesting the relevance of RF secretion to renal organic anion transport. J(bl) was also inhibited by alkaline pH (8.0) and by the calmodulin inhibitor trifluoperazine, indicating the influence of pH and Ca(2+)/calmodulin-dependent pathway on RF secretion. Finally, we found that addition of chlorpromazine, a phenothiazine derivative, inhibited both J(lb) and J(bl), raising the concern about the nutritional status in patients receiving such a type of medication.
Yanagawa
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Medical and Research Services, Sepulveda Veterans Affairs Medical Center, Sepulveda 91343, California, USA. nori@ucla.edu
Shih
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RN
Jo
O D
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Said
H M
HM
D000818:Animals; D014150:Antipsychotic Agents / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects / Q000502:physiology; D002118:Calcium / Q000378:metabolism; D002147:Calmodulin / Q000378:metabolism; D002273:Carcinogens / Q000494:pharmacology; D002746:Chlorpromazine / Q000494:pharmacology; D000242:Cyclic AMP / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005415:Flavins / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007687:Kidney Tubules, Proximal / Q000378:metabolism*; D008297:Male; D010703:Phorbol Esters / Q000494:pharmacology; D011339:Probenecid / Q000494:pharmacology; D011817:Rabbits; D012256:Riboflavin / Q000378:metabolism / Q000493:pharmacokinetics*; D013696:Temperature; D014268:Trifluoperazine / Q000494:pharmacology; D014528:Uricosuric Agents / Q000494:pharmacology; D010130:p-Aminohippuric Acid / Q000494:pharmacology
D014150:Antipsychotic Agents; D002147:Calmodulin; D002273:Carcinogens; D005415:Flavins; D010703:Phorbol Esters; D014528:Uricosuric Agents; C001559:7,8-dimethylalloxazine; D014268:Trifluoperazine; D000242:Cyclic AMP; D011339:Probenecid; D002118:Calcium; D012256:Riboflavin; D002746:Chlorpromazine; D010130:p-Aminohippuric Acid