Chromosome painting reveals specific patterns of chromosome occurrence in mitomycin C- and diethylstilboestrol-induced micronuclei.
Cultures of human blood lymphocytes from three subjects were incubated with the clastogen mitomycin C (MMC, 500 ng/ml) and the aneugen diethylstilboestrol (DES, 80 microM) 23 h before harvesting, to induce formation of micronuclei (MN) and numerical and structural alterations in metaphase chromosomes. We used fluorescence in situ hybridization (FISH) with painting probes for all human chromosomes to determine which chromosomes had contributed material to the induced MN. MMC treatment induced an approximately 18-fold increase in MN and led to a significant increase in hypodiploidy and structural chromosome aberrations in metaphase preparations. Undercondensation of pericentromeric heterochromatin of chromosomes 9 and 1 occurred in 20-75% of metaphases and FISH disclosed an abundance of material from these chromosomes in induced MN (62-69% from chromosome 9 and 7-12% from chromosome 1). DES treatment of lymphocytes induced a seven-fold increase in MN frequency and four-fold increase in the frequency of numerical aberrations; structural aberrations were not significantly increased. FISH analysis showed that material from all chromosomes was present in DES-induced MN, with material from chromosome 1 present in 16% of MN and material from each other chromosomes being present in 2-10% of MN. Material from chromosomes 14, 19 and 21 was significantly more frequent material from chromosome Y significantly less frequent in DES-treated cells than in controls. The findings of the MMC studies indicate that the heterochromatin block of chromosome 9 is a specific target for MMC-induced undercondensation, which induces a preferential occurrence of chromosome 9 material in MN. DES, in contrast, does not trigger heterochromatin decondensation and fails to induce such a significant appearance of material of particular chromosomes in MN.
Fauth
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Abt. Humanbiologie und Humangenetik der Universität, Postfach 3049, D-67653 Kaiserslautern, Germany. efauth@rhrk.uni-kl.de
Scherthan
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Zankl
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D000328:Adult; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D018931:Antineoplastic Agents, Hormonal / Q000494:pharmacology*; D002273:Carcinogens; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D020223:Chromosome Painting; D002878:Chromosomes, Human, Pair 1 / Q000187:drug effects; D002899:Chromosomes, Human, Pair 9 / Q000187:drug effects; D004054:Diethylstilbestrol / Q000494:pharmacology; D005260:Female; D006570:Heterochromatin / Q000378:metabolism; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007211:Indoles / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000235:genetics*; D016685:Mitomycin / Q000494:pharmacology*; D009154:Mutation; D010957:Plasmids / Q000378:metabolism; D011003:Ploidies
D000903:Antibiotics, Antineoplastic; D018931:Antineoplastic Agents, Hormonal; D002273:Carcinogens; D006570:Heterochromatin; D007211:Indoles; C007293:DAPI; D016685:Mitomycin; D004054:Diethylstilbestrol