title: Use of antisense oligodeoxynucleotides to study biological rhythms.
abstract: Perturbation analysis has been crucial in the study of biological rhythms. Antisense technology provides investigators with new means to alter the internal milieu of the circadian clock itself. Practical aspects of the method and the theoretical background are presented in sufficient detail to enable others to design appropriate antisense oligodeoxynucleotides and use them for research purposes. This strategy will contribute substantially to the understanding of the influence of individual genes on rhythms in hormone secretion, metabolism, and behavior.
authors.affiliation: Department of Biology, Rider University, Lawrenceville, New Jersey 08648, USA. kscarbrough@sol.rutgers.edu
authors.forename: K
authors.initials: K
authors.lastname: Scarbrough
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000502:physiology; D002940:Circadian Rhythm / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D006224:Cricetinae; D015195:Drug Design; D020319:Oligodeoxyribonucleotides, Antisense / Q000235:genetics / Q000494:pharmacology*; D010507:Periodicity; D051381:Rats
chemical_list: D020319:Oligodeoxyribonucleotides, Antisense