title: Is there a role for Chlamydia pneumoniae in hemodialysis vascular access thrombosis?
abstract: Significant evidence suggests that Chlamydia pneumoniae has a major role in occlusive vascular disease. Vascular access thrombosis in chronic hemodialysis patients is a frequent problem; the underlying pathological state is stenosis caused by endothelial hyperplasia. There is presently no literature concerning C pneumoniae in vascular access thrombosis. We embarked on a study to evaluate the possible role of C pneumoniae in access failure. Ten consecutive patients with thrombosed polytetrafluoroethylene (PTFE) conduit arteriovenous fistulae undergoing surgical thrombectomy and revision were studied. We sought to detect C pneumoniae using both culture and polymerase chain reaction (PCR) methods. An excisional biopsy of the stenotic vein segment just above the anastomosis with the PTFE graft was obtained at surgery. Vein samples weighing at least 30 mg were aseptically placed in transport media and stored at 4 degrees C for up to 24 hours. The samples then were sonicated, inoculated in Hep-2 cell culture vials containing confluent monolayers, and passaged three times over 2 weeks. Detection was by direct fluorescent antibody staining. Both culture and PCR were performed in an active chlamydia research laboratory. None of the 10 samples was positive for C pneumoniae by culture or PCR. Based on our preliminary pilot study, we do not believe C pneumoniae has a major role in endothelial hyperplasia and consequent graft loss in the hemodialysis patients we studied.
authors.affiliation: Nephrology Associates of Tidewater, Ltd, Norfolk, VA 23507, USA. DrZyd@home.com
authors.forename: A W
authors.initials: AW
authors.lastname: Zydlewski
authors.forename: D R
authors.initials: DR
authors.lastname: Stothard
authors.forename: B
authors.initials: B
authors.lastname: Van Der Pol
authors.forename: R B
authors.initials: RB
authors.lastname: Jones
authors.forename: R S
authors.initials: RS
authors.lastname: Filo
authors.forename: J A
authors.initials: JA
authors.lastname: Hasbargen
mesh_terms: D000368:Aged; D001166:Arteriovenous Shunt, Surgical / Q000009:adverse effects*; D001807:Blood Vessel Prosthesis / Q000009:adverse effects* / Q000382:microbiology; D002408:Catheters, Indwelling / Q000382:microbiology*; D002690:Chlamydia Infections / Q000209:etiology / Q000382:microbiology*; D016993:Chlamydophila pneumoniae / Q000302:isolation & purification*; D004730:Endothelium, Vascular / Q000382:microbiology / Q000601:surgery; D005260:Female; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D006965:Hyperplasia / Q000382:microbiology / Q000601:surgery; D008297:Male; D008875:Middle Aged; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction; D011138:Polytetrafluoroethylene / Q000009:adverse effects; D006435:Renal Dialysis / Q000009:adverse effects*; D013927:Thrombosis / Q000209:etiology / Q000382:microbiology* / Q000601:surgery
chemical_list: D011138:Polytetrafluoroethylene