Human class V alcohol dehydrogenase (ADH5): A complex transcription unit generates C-terminal multiplicity.
The human ADH5 gene was reported to lack the last exon compared to other mammalian ADHs and consequently should be expressed as a truncated protein. Here we show with PCR amplification of 3'-cDNA ends that the ADH5 gene harbors the "missing" exon. Besides a cDNA identical to the published sequence, we found full-length transcripts that contained additional codons for eight amino acid residues. Northern blot analysis established the full-length variant as the major transcript with the strongest signal from adult liver. Sequence analysis of genomic DNA confirmed that the ADH5 gene displays composite internal/terminal exons, which can be differentially processed; i.e., 3'-end generation is a result of competition between polyadenylation and splicing.
Strömberg
P
P
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, SE-171 77, Sweden.
Höög
J O
JO
D000426:Alcohol Dehydrogenase / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D003001:Cloning, Molecular; D005091:Exons; D006801:Humans; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics; D008322:Mammals; D008969:Molecular Sequence Data; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry; D017384:Sequence Deletion; D014158:Transcription, Genetic
D007527:Isoenzymes; D012333:RNA, Messenger; D011994:Recombinant Proteins; D000426:Alcohol Dehydrogenase