Identification of a novel putative Ran-binding protein and its close homologue.
In the process of cloning genes at the breakpoint of t(5;14) (q34;q11), a recurring translocation in acute lymphoblastic leukemia, we isolated and characterized a novel gene at 5q34, and a close human homologue (66% amino acid identity) located at 8p11-12. The presence of an importin-beta N-terminal domain at their N-terminus, their size of approximately 110 kD, their nuclear localization and the identity of the homologue to a gene of a recently submitted RanGTP binding protein (RanBP16), suggest that its protein is a novel member of the importin-beta superfamily of nuclear transport receptors, therefore called RanBP17. Northern blot analysis of human tissues revealed a ubiquitous expression pattern of the RanBP16 gene and a very restricted expression pattern of the RanBP17 gene, showing high expression in testis and pancreas. Both genes are evolutionary conserved and show a high (99 and 94%) amino acid conservation with their murine counterparts and a striking similarity (40%) to a protein product of Caenorhabditis elegans (C35A5.8).
Koch
P
P
Institute of Human Genetics, University of Heidelberg, Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany.
Bohlmann
I
I
Schäfer
M
M
Hansen-Hagge
T E
TE
Kiyoi
H
H
Wilda
M
M
Hameister
H
H
Bartram
C R
CR
Janssen
J W
JW
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D017173:Caenorhabditis elegans; D002467:Cell Nucleus / Q000378:metabolism; D002895:Chromosomes, Human, Pair 5; D002898:Chromosomes, Human, Pair 8; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D015723:Gene Library; D006367:HeLa Cells; D006801:Humans; D017403:In Situ Hybridization; D028884:Karyopherins; D008297:Male; D051379:Mice; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D010179:Pancreas / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012984:Software; D013737:Testis / Q000378:metabolism; D014018:Tissue Distribution; D020931:ran GTP-Binding Protein / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*
D018076:DNA, Complementary; D028884:Karyopherins; D009687:Nuclear Proteins; C417256:RanGTP-binding protein 16; C417257:RanGTP-binding protein 17; C091775:ran-binding protein 1; D020931:ran GTP-Binding Protein