Identification of KLF13 and KLF14 (SP6), novel members of the SP/XKLF transcription factor family.
Using the sequence of the SP1 zinc-finger DNA-binding domain as a probe to screen a mouse EST database, we identified two novel members of the SP/XKLF transcription factor family, KLF13 and KLF14. The mouse Klf13 cDNA (1310 bp in length) contains a single open reading frame of 288 amino acids with a DNA-binding domain closely related to that of the human RFLAT-1 protein and a putative transactivator N-terminal domain rich in proline and alanine residues. The mouse Klf13 gene seems to be the homologue of the human RFLAT1 gene. The mouse Klf14 sequence is homologous to a human genomic sequence from chromosome 17 that is believed to code for a protein with three zinc fingers at the end of its C-terminal domain. Using reverse transcription-polymerase chain reaction, we showed ubiquitous expression of Klf13 and Klf14 in adult mice. A third member of this family was also identified in a human EST database; this sequence was found to be identical to KLF11 (TIEG2), recently identified by Cook et al. (1998, J. Biol. Chem. 273: 25929-25936). The corresponding mouse cDNA was isolated and sequenced. The three genes were localized in the human and the rat: chromosomes 15 (human KLF13), 17q21.3-q22 (human KLF14; HGMW-approved symbol SP6), and 2p25 (human KLF11) and chromosomes 1q31-q32 (rat Klf13), 10q31-q32.1 (rat Klf14) (SP6), and 6q16-q21 (rat Klf11).
Scohy
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Université Libre de Bruxelles, IBMM, Rue Professeurs Jeener & Brachet, 12, Gosselies, B-6041, Belgium.
Gabant
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Van Reeth
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Hertveldt
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Drèze
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Van Vooren
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Rivière
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Szpirer
J
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Szpirer
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D000595:Amino Acid Sequence; D000818:Animals; D051017:Apoptosis Regulatory Proteins; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000235:genetics; D002874:Chromosome Mapping; D002884:Chromosomes, Human, Pair 15; D020224:Expressed Sequence Tags; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D051381:Rats; D012097:Repressor Proteins / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D051703:Sp Transcription Factors; D015534:Trans-Activators / Q000145:classification / Q000235:genetics*; D014157:Transcription Factors / Q000145:classification / Q000235:genetics*; D016335:Zinc Fingers
D051017:Apoptosis Regulatory Proteins; D018797:Cell Cycle Proteins; C115117:KLF11 protein, human; C569356:KLF11 protein, rat; C486423:KLF13 protein, human; C417549:KLF14 protein, human; C486422:Klf13 protein, mouse; D051741:Kruppel-Like Transcription Factors; D012097:Repressor Proteins; D051703:Sp Transcription Factors; C498818:Sp6 protein, mouse; D015534:Trans-Activators; D014157:Transcription Factors