Effect of epinephrine on GLUT2 protein content in mouse liver.
The intraperitoneal administration of epinephrine (EP) to mice caused a significant increase in the blood glucose level and a significant decrease in the liver glycogen content 1 h after its administration at 0.6 mg/kg (blood glucose; p < 0.01, liver glycogen; p < 0.01). The liver type glucose transporter (GLUT2) mRNA expression and protein content from mouse liver significantly increased in the intraperitoneally EP-treated mice when compared to that in normal mice (mRNA; p < 0.01, protein; p < 0.01). These results suggest that the hyperglycemic effect of EP is due, at least in part, to the increase of GLUT2 protein synthesis.
Miura
T
T
Department of Clinical Nutrition, Suzuka University of Medical Science, 1001-1 Kishioka-cho, Suzuka, Mie 510-0293, Japan. miura@suzuka-u.ac.jp
Kato
M
M
Iwamoto
N
N
Yamada
Y
Y
Ishida
T
T
Seino
Y
Y
Tanigawa
K
K
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D015152:Blotting, Northern; D015153:Blotting, Western; D004837:Epinephrine / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D051273:Glucose Transporter Type 2; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008112:Liver Glycogen / Q000378:metabolism; D008297:Male; D051379:Mice; D009004:Monosaccharide Transport Proteins / Q000096:biosynthesis / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
D001786:Blood Glucose; D051273:Glucose Transporter Type 2; D008112:Liver Glycogen; D009004:Monosaccharide Transport Proteins; D012333:RNA, Messenger; D005947:Glucose; D004837:Epinephrine