title: The S. pombe rlc1 gene encodes a putative myosin regulatory light chain that binds the type II myosins myo3p and myo2p.
abstract: In order to identify additional components important for cell division in the fission yeast Schizosaccharomyces pombe we have screened a bank of conditional cold-sensitive mutants for cytokinesis defects. One of these mutants showed a delay in cell cleavage, and strong genetic interactions with other genes implicated in medial ring formation. Cloning of the corresponding gene indicates that it encodes a protein with significant homology to the regulatory light chain of non-muscle myosins. We have named the gene rlc1 (regulatory light chain 1). The gene is not essential for division, but null mutants display a cell cleavage defect and form an aberrant F-actin ring. Two myosin-II heavy chains have been identified in fission yeast: Co-immunoprecipitation experiments indicate that rlc1p associates more strongly with myo3p than myo2p.
authors.affiliation: Cell Cycle Control Laboratory, Swiss Institute for Experimental Cancer Research (ISREC), Switzerland.
authors.forename: X
authors.initials: X
authors.lastname: Le Goff
authors.forename: F
authors.initials: F
authors.lastname: Motegi
authors.forename: E
authors.initials: E
authors.lastname: Salimova
authors.forename: I
authors.initials: I
authors.lastname: Mabuchi
authors.forename: V
authors.initials: V
authors.lastname: Simanis
mesh_terms: D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000378:metabolism*; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000378:metabolism*; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D007202:Indicators and Reagents / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D016296:Mutagenesis / Q000502:physiology; D018995:Myosin Heavy Chains / Q000378:metabolism*; D018994:Myosin Light Chains / Q000235:genetics* / Q000378:metabolism*; D024462:Myosin Type II; D024701:Myosin Type V; D009218:Myosins / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism*; D010641:Phenotype; D010766:Phosphorylation; D011485:Protein Binding / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D012568:Schizosaccharomyces; D029702:Schizosaccharomyces pombe Proteins
chemical_list: D002352:Carrier Proteins; D005656:Fungal Proteins; D007202:Indicators and Reagents; D008164:Luminescent Proteins; C077512:MYO2 protein, S cerevisiae; C434211:MYO2 protein, S pombe; D018994:Myosin Light Chains; C110514:Myp2 protein, S pombe; D009419:Nerve Tissue Proteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; C490463:rlc1 protein, S pombe; D049452:Green Fluorescent Proteins; D024462:Myosin Type II; D024701:Myosin Type V; D018995:Myosin Heavy Chains; D009218:Myosins