Effect of peptidyltransferase inhibitors on ribonuclease P activity from Dictyostelium discoideum. Effect of antibiotics on RNase P.
The effect of several peptidyltransferase inhibitors on ribonuclease P activity from Dictyostelium discoideum was investigated. Among the inhibitors tested puromycin, amicetin and blasticidin S revealed a dose-dependent inhibition of tRNA maturation. Blasticidin S and amicetin do not compete with puromycin for the same site on the enzyme, suggesting the existence of distinct antibiotic binding sites on D. discoideum RNase P. Inhibition experiments further indicate that binding sites for blasticidin S and amicetin overlap.
Stathopoulos
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Deparment of Biochemistry, School of Medicine, University of Patras, Greece.
Tsagla
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Tekos
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Drainas
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D
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002474:Cell-Free System; D004023:Dictyostelium / Q000201:enzymology*; D004722:Endoribonucleases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D009705:Nucleosides / Q000494:pharmacology; D010458:Peptidyl Transferases / Q000037:antagonists & inhibitors*; D011691:Puromycin / Q000494:pharmacology; D011741:Pyrimidine Nucleosides / Q000494:pharmacology; D016337:RNA, Catalytic / Q000037:antagonists & inhibitors / Q000378:metabolism*; D043262:Ribonuclease P
D000900:Anti-Bacterial Agents; D004791:Enzyme Inhibitors; D009705:Nucleosides; D011741:Pyrimidine Nucleosides; D016337:RNA, Catalytic; C004413:amicetin; D011691:Puromycin; C004500:blasticidin S; D010458:Peptidyl Transferases; D004722:Endoribonucleases; D043262:Ribonuclease P