Human CD8(+) T cells specific for Mycobacterium tuberculosis secreted antigens in tuberculosis patients and healthy BCG-vaccinated controls in The Gambia.
Intracellular flow cytometry analysis of perforin production by CD8(+) T cells showed levels were greatly reduced in tuberculosis (TB) patients compared to healthy controls. Reduced cytotoxic-T-lymphocyte activity was also obtained with CD8(+) T cells from TB patients compared to healthy controls in The Gambia. A change in antigen recognition was noted between the two groups of donors: in addition to recognition of Ag85A and Ag85B, as seen in healthy donors, a prominent ESAT-6 response was found in TB patients.
Smith
S M
SM
Immunology Unit, London School of Hygiene & Tropical Medicine, London, United Kingdom. steven.smith@lshtm.ac.uk
Klein
M R
MR
Malin
A S
AS
Sillah
J
J
Huygen
K
K
Andersen
P
P
McAdam
K P
KP
Dockrell
H M
HM
D000328:Adult; D000942:Antigens, Bacterial / Q000276:immunology*; D001500:BCG Vaccine / Q000276:immunology*; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D054353:Perforin; D052899:Pore Forming Cytotoxic Proteins; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014376:Tuberculosis / Q000276:immunology*; D014611:Vaccination
D000942:Antigens, Bacterial; D001500:BCG Vaccine; D008562:Membrane Glycoproteins; D052899:Pore Forming Cytotoxic Proteins; C065036:antigen 85, Mycobacterium bovis; D054353:Perforin