title: Oxidative stress and hypoxia-like injury cause Alzheimer-type molecular abnormalities in central nervous system neurons.
abstract: Neuronal loss and neuritic/cytoskeletal lesions (synaptic disconnection and proliferation of dystrophic neurites) represent major dementia-associated abnormalities in Alzheimer's disease (AD). This study examined the role of oxidative stress as a factor contributing to both the cell death and neuritic degeneration cascades in AD. Primary neuron cultures were treated with H2O2 (9-90 microM) or desferrioxamine (2-25 microM) for 24 h and then analyzed for viability, mitochondrial mass, mitochondrial function, and pro-apoptosis and sprouting gene expression. H2O2 treatment causes free-radical injury and desferrioxamine causes hypoxia-type injury without free radical generation. The H2O2-treated cells exhibited sustained viability but neurite retraction, impaired mitochondrial function, increased levels of the pro-apoptosis gene product CD95/Fas, reduced expression of N2J1-immunoreactive neuronal thread protein and synaptophysin, and reduced distribution of mitochondria in neuritic processes. Desferrioxamine treatment resulted in dose-dependent neuronal loss associated with impaired mitochondrial function, proliferation of neurites, and reduced expression of GAP-43, which has a role in path-finding during neurite outgrowth. The results suggest that oxidative stress can cause neurodegeneration associated with enhanced susceptibility to apoptosis due to activation of pro-apoptosis genes, neurite retraction (synaptic disconnection), and impaired transport of mitochondria to cell processes where they are likely required for synaptic function. In contrast, hypoxia-type injury causes neuronal loss with proliferation of neurites (sprouting), impaired mitochondrial function, and reduced expression of molecules required to form and maintain synaptic connections. Since similar abnormalities occur in AD, both oxidative stress and hypoxic injury can contribute to AD neurodegeneration.
authors.affiliation: Department of Medicine, Rhode Island Hospital, Brown University School of Medicine, Providence 02903, USA. delamonte@hotmail.com
authors.forename: S M
authors.initials: SM
authors.lastname: de la Monte
authors.forename: T R
authors.initials: TR
authors.lastname: Neely
authors.forename: J
authors.initials: J
authors.lastname: Cannon
authors.forename: J R
authors.initials: JR
authors.lastname: Wands
mesh_terms: D000544:Alzheimer Disease / Q000209:etiology* / Q000473:pathology; D000818:Animals; D017209:Apoptosis; D002455:Cell Division; D015687:Cell Hypoxia; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002490:Central Nervous System / Q000473:pathology*; D003676:Deferoxamine / Q000494:pharmacology; D003576:Electron Transport Complex IV / Q000096:biosynthesis; D005456:Fluorescent Dyes; D005609:Free Radicals; D015870:Gene Expression; D049452:Green Fluorescent Proteins; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008164:Luminescent Proteins; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D009410:Nerve Degeneration; D009474:Neurons / Q000473:pathology*; D009930:Organic Chemicals; D018384:Oxidative Stress; D051381:Rats; D019014:fas Receptor / Q000096:biosynthesis
chemical_list: D005456:Fluorescent Dyes; D005609:Free Radicals; D008164:Luminescent Proteins; D009930:Organic Chemicals; D019014:fas Receptor; C107472:red dye CMXRos; D049452:Green Fluorescent Proteins; D006861:Hydrogen Peroxide; D003576:Electron Transport Complex IV; D003676:Deferoxamine