Molecular analysis of the h-warts/LATS1 gene in human breast cancer.
Loss of heterozygosity (LOH) on chromosome 6q is often observed in breast cancer, suggesting the existence of a putative tumor suppressor. Recently, a human homolog of the Drosophila warts tumor suppressor gene, h-warts/LATS1, was identified and mapped at chromosome 6q24-25.1. Mutation analysis of the h-warts/LATS1 was performed using 25 breast cancer tissues by RT-PCR SSCP analysis. Although LOH of the h-warts/LATS1 was found in one patient, no mutations were found. Two polymorphisms were found, but neither of them caused amino acid substitutions. Further investigations are necessary to elucidate the role of the h-warts/LATS1 gene in the carcinogenesis of breast cancer.
Morinaga
N
N
Department of Surgery I, Faculty of Medicine, Gunma University, Maebashi, Gunma 371-8511, Japan. nmoring@med.gunma-u.ac.jp
Shitara
Y
Y
Yanagita
Y
Y
Koida
T
T
Kimura
M
M
Asao
T
T
Kimijima
I
I
Takenoshita
S
S
Hirota
T
T
Saya
H
H
Kuwano
H
H
D001943:Breast Neoplasms / Q000737:chemistry / Q000235:genetics* / Q000648:ultrastructure; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics*; D004252:DNA Mutational Analysis; D018076:DNA, Complementary / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D029721:Drosophila Proteins; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D006801:Humans; D019656:Loss of Heterozygosity; D009363:Neoplasm Proteins / Q000032:analysis / Q000235:genetics*; D011110:Polymorphism, Genetic; D018807:Polymorphism, Single-Stranded Conformational; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases / Q000032:analysis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013347:Subcellular Fractions / Q000737:chemistry
D018076:DNA, Complementary; D004273:DNA, Neoplasm; D029721:Drosophila Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011494:Protein Kinases; C092745:LATS1 protein, human; C093503:wts protein, Drosophila; D017346:Protein Serine-Threonine Kinases