Sequence differences in the diagnostic target region of the oocyst wall protein gene of Cryptosporidium parasites.
Nucleotide sequences of the Cryptosporidium oocyst wall protein (COWP) gene were obtained from various Cryptosporidium spp. (C. wrairi, C. felis, C. meleagridis, C. baileyi, C. andersoni, C. muris, and C. serpentis) and C. parvum genotypes (human, bovine, monkey, marsupial, ferret, mouse, pig, and dog). Significant diversity was observed among species and genotypes in the primer and target regions of a popular diagnostic PCR. These results provide useful information for COWP-based molecular differentiation of Cryptosporidium spp. and genotypes.
Xiao
L
L
National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Atlanta, Georgia 30341, USA. LAX0@CDC.GOV
Limor
J
J
Morgan
U M
UM
Sulaiman
I M
IM
Thompson
R C
RC
Lal
A A
AA
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003458:Cryptosporidium / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics; D004285:Dogs; D017125:Genes, Protozoan; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015800:Protozoan Proteins / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D017931:DNA Primers; D016054:DNA, Protozoan; D015800:Protozoan Proteins; C106305:oocyst wall protein, Cryptosporidium