title: Morphological observation on cell death and phagocytosis induced by ultraviolet irradiation in a cultured human lens epithelial cell line.
abstract: The purpose of this study is to observe dynamic morphological changes induced by ultraviolet (UV) irradiation in a cultured human lens epithelial cell line using electron microscopy, cell viability staining, time-lapsed videography and immunohistochemistry. Human lens epithelial cell line SRA 01-04 was cultured in Dulbecco's Modified Eagle Medium (DMEM) containing 20% fetal bovine serum. Subconfluent cells were irradiated under a bank of UV lamps, which emitted 275-400 nm radiation with a maximum at 310 nm. The UV intensity was 20 microW cm(-2)at dosages from 0 to 10 mJ cm(-2). Alterations in the morphology of the living cells were monitored and recorded with phase-contrast microscopy and time-lapsed videography. At different times, the cells were fixed and examined by transmission electron microscopy (TEM), diamidinophenolindole (DAPI) staining, and in situ immunohistochemistry using TdT-mediated dUTP-biotin nick end labeling (TUNEL). Cell viability was also assessed with crystal violet staining. At low doses of UV exposure (2-5 mJ cm(-2)), time-lapsed videography revealed definitive cell death that appeared to be primarily apoptotic. The dead cell debris was engulfed and phagocytosed by neighboring living cells. Phase-contrast microscopy and TEM demonstrated that, at UV 10 mJ cm(-2), the cells not only showed typical apoptosis such as nuclear membrane shrinkage, chromatin condensation, and fragmentation into apoptotic bodies, but also necrosis such as swelling of the nucleus and cell body, and disruption of the plasma membrane. In support, DNA staining and in situ immunohistochemical reactions in the UV irradiated cells were both positive. The phagocytotic process was also seen with TEM. UV irradiation thus appears to cause both apoptosis and necrosis in the cultured human lens epithelial cell line. Active migration and phagocytosis of the cells appear to be stimulated by UV-induced damage. These findings may also aid in the understanding of UV injury and repair mechanisms of lens epithelial cells in vivo.
authors.affiliation: Department of Ophthalmology, Kanazawa Medical University, Uchinada, Japan. shui@vision.wustl.edu
authors.forename: Y B
authors.initials: YB
authors.lastname: Shui
authors.forename: H
authors.initials: H
authors.lastname: Sasaki
authors.forename: J H
authors.initials: JH
authors.lastname: Pan
authors.forename: I
authors.initials: I
authors.lastname: Hata
authors.forename: M
authors.initials: M
authors.lastname: Kojima
authors.forename: Y
authors.initials: Y
authors.lastname: Yamada
authors.forename: K I
authors.initials: KI
authors.lastname: Hirai
authors.forename: N
authors.initials: N
authors.lastname: Takahashia
authors.forename: K
authors.initials: K
authors.lastname: Sasaki
mesh_terms: D017209:Apoptosis / Q000528:radiation effects; D002465:Cell Movement / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000473:pathology / Q000528:radiation effects*; D006801:Humans; D007150:Immunohistochemistry; D007908:Lens, Crystalline / Q000166:cytology / Q000528:radiation effects*; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast; D018715:Microscopy, Video; D009336:Necrosis; D010587:Phagocytosis / Q000528:radiation effects*; D014466:Ultraviolet Rays