Transient modulation of gene expression in the neurulation staged mouse embryo.
Transient modulation of gene expression in the embryo during early organogenesis will allow studies to be conducted that determine tissue- and stage-specific function(s) of genes. To achieve this goal, viral vectors and antisense oligodeoxynucleotides have been used to produce gain-of-function and loss-of-function models. Adenoviral transduction of whole embryos, embryonic heart and vasculature, and primary neural crest cell culture has been reported. The morphological consequences of overexpression or decreasing expression of selected genes have been evaluated using these tools. Gene-teratogen interaction studies have also been performed. The viral vectors appear to be important tools for modulating gene expression and hold great promise for future research.
Hunter
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Reproductive Toxicology Division, National Health and Environmental Effects Research Laboratory, United States Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA. Hunter.sid@epa.gov
Hartig
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D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D004622:Embryo, Mammalian / Q000187:drug effects / Q000196:embryology* / Q000378:metabolism*; D005314:Embryonic and Fetal Development / Q000187:drug effects / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000235:genetics*; D017930:Genes, Reporter; D005822:Genetic Vectors; D051379:Mice; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D013723:Teratogens / Q000494:pharmacology / Q000633:toxicity; D014161:Transduction, Genetic
D016376:Oligonucleotides, Antisense; D013723:Teratogens