Role for trehalase during germination of spores in the fission yeast Schizosaccharomyces pombe.
Spores from Schizosaccharomyces pombe contain neutral and acid trehalases. When spores from strains disrupted for ntp1(+), which encodes neutral trehalase, were induced to germinate, the onset of the process was markedly delayed as compared to wild-type spores. Further outgrowth was also reduced. Dormant spores lacking neutral trehalase contained twice the amount of trehalose present in wild-type spores and mobilised the intracellular pool of trehalose at a slower rate during germination. Inhibition by phloridzin of the sporulation-specific acid trehalase in ntp1-disrupted spores arrested germination completely while prompting no effect on wild-type spores. These results suggest that the two trehalase enzymes may support the utilisation of trehalose during germination but neutral trehalase is required for a more rapid and efficient process.
Beltran
F F
FF
Department of Genetics and Microbiology, Faculty of Biology, University of Murcia, 30071, Murcia, Spain.
Castillo
R
R
Vicente-Soler
J
J
Cansado
J
J
Gacto
M
M
D003470:Culture Media; D004791:Enzyme Inhibitors / Q000494:pharmacology; D009154:Mutation; D010695:Phlorhizin / Q000494:pharmacology; D012568:Schizosaccharomyces / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D013172:Spores, Fungal / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D014198:Trehalase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014199:Trehalose / Q000378:metabolism
D003470:Culture Media; D004791:Enzyme Inhibitors; D014199:Trehalose; D010695:Phlorhizin; D014198:Trehalase