Cloning and sequence analysis of the rat norepinephrine transporter promoter.
We isolated a 2.5-kb fragment of the promoter for the rat norepinephrine transporter (NET) gene. The transcription start site was identified approximately 200 base pairs upstream from the translation start site. Several potential regulatory elements were identified by sequence analysis. The structure of the rat NET promoter was compared to mouse and human. Expression studies in placental and neuroblastoma cells suggested the presence of a 'repressor' element more than 500 base pairs upstream from the transcription start site. These studies provide the basis for examination of transcriptional regulation of this gene and for understanding its temporal and tissue-specific modes of regulation.
Padbury
J F
JF
Department of Pediatrics, Brown University School of Medicine, Women and Infants' Hospital of Rhode Island, Providence, RI, USA. james_padbury@brown.edu
McGonnigal
B
B
Tseng
Y T
YT
Nguyen
T T
TT
Stabila
J P
JP
D020121:5' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D006801:Humans; D007438:Introns; D008156:Luciferases / Q000235:genetics; D008969:Molecular Sequence Data; D009447:Neuroblastoma; D050484:Norepinephrine Plasma Membrane Transport Proteins; D011401:Promoter Regions, Genetic / Q000235:genetics*; D051381:Rats; D017422:Sequence Analysis, DNA; D027981:Symporters; D014158:Transcription, Genetic / Q000187:drug effects / Q000235:genetics; D014407:Tumor Cells, Cultured
D020121:5' Untranslated Regions; D002352:Carrier Proteins; D050484:Norepinephrine Plasma Membrane Transport Proteins; C493227:SLC6A2 protein, human; C493226:Slc6a2 protein, rat; D027981:Symporters; D005576:Colforsin; D000242:Cyclic AMP; D008156:Luciferases