title: Local control of neurofilament accumulation during radial growth of myelinating axons in vivo. Selective role of site-specific phosphorylation.
abstract: The accumulation of neurofilaments required for postnatal radial growth of myelinated axons is controlled regionally along axons by oligodendroglia. Developmentally regulated processes previously suspected of modulating neurofilament number, including heavy neurofilament subunit (NFH) expression, attainment of mature neurofilament subunit stoichiometry, and expansion of interneurofilament spacing cannot be primary determinants of regional accumulation as we show each of these factors precede accumulation by days or weeks. Rather, we find that regional neurofilament accumulation is selectively associated with phosphorylation of a subset of Lys-Ser-Pro (KSP) motifs on heavy neurofilament subunits and medium-size neurofilament subunits (NFMs), rising >50-fold selectively in the expanding portions of optic axons. In mice deleted in NFH, substantial preservation of regional neurofilament accumulation was accompanied by increased levels of the same phosphorylated KSP epitope on NFM. Interruption of oligodendroglial signaling to axons in Shiverer mutant mice, which selectively inhibited this site-specific phosphorylation, reduced regional neurofilament accumulation without affecting other neurofilament properties or aspects of NFH phosphorylation. We conclude that phosphorylation of a specific KSP motif triggered by glia is a key aspect of the regulation of neurofilament number in axons during axonal radial growth.
authors.affiliation: Department of Psychiatry, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: I
authors.initials: I
authors.lastname: Sánchez
authors.forename: L
authors.initials: L
authors.lastname: Hassinger
authors.forename: R K
authors.initials: RK
authors.lastname: Sihag
authors.forename: D W
authors.initials: DW
authors.lastname: Cleveland
authors.forename: P
authors.initials: P
authors.lastname: Mohan
authors.forename: R A
authors.initials: RA
authors.lastname: Nixon
mesh_terms: D000818:Animals; D002450:Cell Communication / Q000502:physiology; D000939:Epitopes / Q000378:metabolism; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008818:Mice, Neurologic Mutants; D009186:Myelin Sheath / Q000235:genetics; D009413:Nerve Fibers, Myelinated / Q000378:metabolism*; D016900:Neurofilament Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism / Q000648:ultrastructure; D009836:Oligodendroglia / Q000166:cytology / Q000502:physiology; D009900:Optic Nerve / Q000166:cytology / Q000254:growth & development; D010766:Phosphorylation; D017434:Protein Structure, Tertiary
chemical_list: D000939:Epitopes; D016900:Neurofilament Proteins; C051774:neurofilament protein H