Characterization of a cDNA encoding a 72 kDa heat shock cognate protein (Hsc72) from the Pacific oyster, Crassostrea gigas.
A full-length cDNA encoding a 72 kDa heat shock cognate protein (Hsc72) was isolated from a Crassostrea gigas hemocyte library. This cDNA is 75% identical to a human hsc70 cDNA. C. gigas cDNA contains a 659 amino acid open reading frame encoding a 72 kDa protein which is 87% identical to a human Hsc70 protein. Northern blotting indicated that even if the hsc72 gene was constitutively expressed in oyster hemocytes, it could be stimulated by heat shock in vitro as well as in vivo. Homologies observed both at the cDNA and protein levels, as well as the expression pattern of the hsc72 mRNA, support our conclusion that the isolated cDNA clone corresponds to an oyster heat shock cognate gene.
Gourdon
I
I
Laboratoire de Défense et Résistance chez les Invertébrés Marins, Université de Montpellier II CC80, France.
Gricourt
L
L
Kellner
K
K
Roch
P
P
Escoubas
J M
JM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics; D018076:DNA, Complementary; D015723:Gene Library; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics*; D006434:Hemocytes / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009928:Organ Specificity; D010125:Ostreidae / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D002352:Carrier Proteins; D018076:DNA, Complementary; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; D050884:HSP72 Heat-Shock Proteins; C494371:HSPA8 protein, human; D006360:Heat-Shock Proteins