title: Macaque blood-derived antigen-presenting cells elicit SIV-specific immune responses.
abstract: Natural blood-borne antigen-presenting cells (APCs) were tested for their ability to augment antigen presentation for SIV vaccines. Fibrocytes and monocyte-derived dendritic cells (DCs) were isolated from multiple Macaca fascicularis. Macaque fibrocytes displayed the characteristic cellular morphology and stained positive for CD34 and collagen, as observed in human and murine fibrocytes. Macaque DCs were generated from monocytes by culturing in granulocyte-macrophage colony stimulating factor and interleukin-4 (IL-4). Two days after maturation, cells were enriched for the DC marker CD83. Fibrocytes and DCs were each transfected with green fluorescence protein expression plasmids or DNA expression vectors encoding all of the SIVmne structural and regulatory genes. Autologous DCs were re-infused into macaques subcutaneously (sc) following transfection; mixing with recombinant SIV antigens or inactivated whole SIV in vitro; or mock-treatment. Autologous monocyte-derived DCs pulsed with whole inactivated SIV were re-infused and elicited cellular and/or humoral responses in vivo in eight of ten vaccinated macaques.
authors.affiliation: Seattle Biomedical Research Institute, Seattle, WA 98109, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhu
authors.forename: K
authors.initials: K
authors.lastname: Koo
authors.forename: J D
authors.initials: JD
authors.lastname: Bradshaw
authors.forename: W F
authors.initials: WF
authors.lastname: Sutton
authors.forename: L R
authors.initials: LR
authors.lastname: Kuller
authors.forename: R
authors.initials: R
authors.lastname: Bucala
authors.forename: D
authors.initials: D
authors.lastname: Anderson
authors.forename: S P
authors.initials: SP
authors.lastname: Mossman
authors.forename: F
authors.initials: F
authors.lastname: Villinger
authors.forename: N L
authors.initials: NL
authors.lastname: Haigwood
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000938:Antigen-Presenting Cells / Q000276:immunology* / Q000821:virology; D015703:Antigens, CD; D018952:Antigens, CD34 / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology / Q000637:transplantation / Q000821:virology; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D006801:Humans; D007111:Immunity, Cellular; D007136:Immunoglobulins / Q000032:analysis; D008164:Luminescent Proteins / Q000032:analysis; D008252:Macaca fascicularis; D008562:Membrane Glycoproteins / Q000032:analysis; D009000:Monocytes / Q000276:immunology; D018449:SAIDS Vaccines / Q000276:immunology*; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology*; D015302:Simian Immunodeficiency Virus / Q000276:immunology*; D014162:Transfection; D014182:Transplantation, Autologous; D000097802:CD83 Antigen
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34; D000956:Antigens, Viral; D049452:Green Fluorescent Proteins; D007136:Immunoglobulins; D008164:Luminescent Proteins; D008562:Membrane Glycoproteins; D018449:SAIDS Vaccines; D000097802:CD83 Antigen