Rapid determination of protein folds using residual dipolar couplings.
Over the next few years, various genome projects will sequence many new genes and yield many new gene products. Many of these products will have no known function and little, if any, sequence homology to existing proteins. There is reason to believe that a rapid determination of a protein fold, even at low resolution, can aid in the identification of function and expedite the determination of structure at higher resolution. Recently devised NMR methods of measuring residual dipolar couplings provide one route to the determination of a fold. They do this by allowing the alignment of previously identified secondary structural elements with respect to each other. When combined with constraints involving loops connecting elements or other short-range experimental distance information, a fold is produced. We illustrate this approach to protein fold determination on (15)N-labeled Eschericia coli acyl carrier protein using a limited set of (15)N-(1)H and (1)H-(1)H dipolar couplings. We also illustrate an approach using a more extended set of heteronuclear couplings on a related protein, (13)C, (15)N-labeled NodF protein from Rhizobium leguminosarum.
Fowler
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Complex Carbohydrate Research Center, The University of Georgia, 220 Riverbend Road, Athens, GA, 30602-4712, USA.
Tian
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Al-Hashimi
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Prestegard
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D000213:Acyl Carrier Protein / Q000737:chemistry* / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D002247:Carbon Isotopes / Q000378:metabolism; D003903:Deuterium / Q000378:metabolism; D004926:Escherichia coli / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D009587:Nitrogen Isotopes / Q000378:metabolism; D017510:Protein Folding; D017433:Protein Structure, Secondary; D016961:Rhizobium leguminosarum / Q000737:chemistry*; D012984:Software
D000213:Acyl Carrier Protein; D001426:Bacterial Proteins; D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; C068605:nodF protein, Rhizobium; D003903:Deuterium