Cutting edge: heat shock protein gp96 induces maturation and migration of CD11c+ cells in vivo.
Immunization of mice with the heat shock protein (HSP) gp96 but not control proteins leads to 5- to 7-fold enlargement of draining lymph nodes (LNs) resulting from accumulation of large numbers of mature CD11c(+) cells, but not T or B lymphocytes in them. The increase in size and cellularity is time-dependent; the draining LNs reach their peak size between 12 and 24 h after injection and regress to their normal size between 48 and 72 h after injection. The increment is elicited specifically in the draining LN but not in other LNs. This observation uncovers a novel aspect of HSP-APC interaction and adds to the mechanistic explanation for the unusually high immunogenicity of HSP-peptide complexes.
Binder
R J
RJ
Center for Immunotherapy of Cancer and Infectious Diseases, University of Connecticut School of Medicine, Farmington, CT 06030, USA.
Anderson
K M
KM
Basu
S
S
Srivastava
P K
PK
D000818:Animals; D000938:Antigen-Presenting Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D000951:Antigens, Neoplasm / Q000008:administration & dosage* / Q000276:immunology / Q000502:physiology*; D002454:Cell Differentiation / Q000276:immunology; D002455:Cell Division / Q000276:immunology; D002465:Cell Movement / Q000276:immunology*; D006133:Growth Substances / Q000008:administration & dosage* / Q000276:immunology / Q000502:physiology*; D006360:Heat-Shock Proteins / Q000008:administration & dosage* / Q000276:immunology / Q000502:physiology*; D007271:Injections, Intradermal; D016167:Integrin alphaXbeta2 / Q000096:biosynthesis*; D008198:Lymph Nodes / Q000033:anatomy & histology / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009929:Organ Size / Q000276:immunology
D000951:Antigens, Neoplasm; D006133:Growth Substances; D006360:Heat-Shock Proteins; D016167:Integrin alphaXbeta2; C052646:sarcoma glycoprotein gp96 rejection antigens