Fluorescence polarization and anisotropy in high throughput screening: perspectives and primer.
Fluorescence polarization and anisotropy are two nearly equivalent techniques that have together, over the past 5 years, achieved wide use in high throughput screening in drug discovery. These are single-label methods that can be used to construct homogeneous assays that are fast, sensitive, and resistant to some significant interferences. Moreover, the assays are relatively inexpensive. This review surveys the peer-reviewed literature on the subject and explores some of the fundamental issues that bear on assay performance.
Owicki
J C
JC
jack@owicki.com
D001667:Binding, Competitive; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation / Q000379:methods*; D005453:Fluorescence; D005454:Fluorescence Polarization / Q000295:instrumentation / Q000379:methods*; D008024:Ligands; D011956:Receptors, Cell Surface / Q000378:metabolism; D012107:Research Design; D012680:Sensitivity and Specificity
D008024:Ligands; D011956:Receptors, Cell Surface