Spectroscopic techniques in the study of membrane solubilization, reconstitution and permeabilization by detergents.
This review focuses on the use of spectroscopic techniques for the study of membrane solubilization, reconstitution, and permeabilization by detergents. Turbidity and light scattering, visible and infrared spectroscopic methods, fluorescence, nuclear magnetic resonance, electron spin resonance and X-ray diffraction are examined from the point of view of their applicability to the above detergent-mediated phenomena. A short introduction is provided about each of the techniques, and references are given for further study.
Goñi
F M
FM
Unidad de Biofísica (CSIC-UPV/EHU) and Departamento de Bioquímica, Universidad del País Vasco, Aptdo. 644, 48080, Bilbao, Spain.
Alonso
A
A
D003902:Detergents / Q000737:chemistry* / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy; D008081:Liposomes / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008567:Membranes, Artificial; D009391:Nephelometry and Turbidimetry; D010539:Permeability / Q000187:drug effects; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D013055:Spectrophotometry, Infrared; D013057:Spectrum Analysis / Q000379:methods*; D014961:X-Ray Diffraction
D003902:Detergents; D008081:Liposomes; D008567:Membranes, Artificial