title: Electrochemical assay of endo-depolymerase activity of cellulases.
abstract: A method for determination of endo-1,4-beta-D-glucanase activity of cellulase samples based on the indirect measurement of decrease in viscosity of a carboxymethylcellulose solution in an electrochemical cell in the presence of an electron carrier was developed. A rotating disk electrode is used as the working electrode. When two reactions (enzymatic and electrochemical) proceeded in the cell simultaneously, the limiting diffusion current at a constant applied potential increases as the viscosity of the solution decreases. Conditions where the initial rate of change of diffusion current (dI/dt) is proportional to the enzyme concentration were found. A good correlation between the new method and a previously known viscometric method for determination of endoglucanase activity was observed.
authors.affiliation: School of Chemistry, Lomonosov Moscow State University, Moscow, 119899, Russia. askomarovsky@enzyme.chem.msu.ru
authors.forename: A A
authors.initials: AA
authors.lastname: Skomarovsky
authors.forename: I N
authors.initials: IN
authors.lastname: Zorov
authors.forename: S G
authors.initials: SG
authors.lastname: Grishutin
authors.forename: A V
authors.initials: AV
authors.lastname: Markov
authors.forename: A P
authors.initials: AP
authors.lastname: Sinitsyn
authors.forename: A V
authors.initials: AV
authors.lastname: Gusakov
authors.forename: A D
authors.initials: AD
authors.lastname: Ryabov
authors.forename: B B
authors.initials: BB
authors.lastname: Gnedenko
mesh_terms: D002266:Carboxymethylcellulose Sodium; D002480:Cellulase / Q000032:analysis / Q000378:metabolism* / Q000592:standards; D004563:Electrochemistry / Q000295:instrumentation; D012015:Reference Standards; D014783:Viscosity
chemical_list: D002480:Cellulase; D002266:Carboxymethylcellulose Sodium