title: Endoscope washers--a protocol for their use.
abstract: A protocol for the disinfection of gastroduodenoscopes, retrograde cholangiopancreatography endoscopes and colonoscopes using endoscope washers is described. The process recommends initial manual washing with a disinfectant containing didecyldimethylammonium chloride, surfactants and enzymes, a second washing in the endoscope washer using a detergent associated with a bacteriostatic, fungistatic substance (benzoisothiazolone) and finally the use of a 2% glutaraldehyde product buffered at pH6. After treatment with 2% Steranios added to the washer, less than 1 micro-organism/ml liquid was found in the following units: 83% of the colonoscopes, 83% of the oesophagogastroduodenoscopes, 83% of the main channels of the retrograde cholangiopancreatography endoscopes and 75% of the auxiliary channels of the latter instruments. In 14% of the colonoscopes, 42% of the gastroduodenoscopes, 42% of the main and 50% of auxiliary channels in the retrograde cholangiopancreatography endoscopes there were no signs of microbial growth in the wash liquid. The results obtained indicated that this protocol allowed adequate disinfection of the endoscope channels, structurally the most difficult part of the instrument to disinfect. Emphasis is given to the degree by which instrument contamination can increase during overnight storage, suggesting that endoscopes need to be submitted to further disinfection after overnight storage. Moreover, the water flowing into the washers can also cause instrument recontamination, particularly during the final rinses. Therefore, to better safeguard the health of patients undergoing endoscopy, special care must be taken to maintain the filters and disinfect the washers themselves, no matter how effective the disinfection protocol used may be.
authors.affiliation: Cattedra di Citologia ed Istologia, Università di, Ferrara, Italy.
authors.forename: M G
authors.initials: MG
authors.lastname: Marchetti
authors.forename: G
authors.initials: G
authors.lastname: Salvatorelli
authors.forename: G
authors.initials: G
authors.lastname: Finzi
authors.forename: P
authors.initials: P
authors.lastname: Cugini
mesh_terms: D002760:Cholangiopancreatography, Endoscopic Retrograde / Q000295:instrumentation; D015169:Colony Count, Microbial; D004203:Disinfection / Q000295:instrumentation / Q000379:methods / Q000592:standards*; D020776:Endoscopes, Gastrointestinal / Q000382:microbiology*; D016099:Endoscopy, Gastrointestinal; D004866:Equipment Contamination / Q000517:prevention & control*; D004865:Equipment and Supplies, Hospital / Q000382:microbiology; D006801:Humans