The novel peptide composition of the seeds of Trichosanthes dioica Roxb.
The seeds of Trichosanthes dioica contain a large amount of peptides in the range of 2-8 kD. These peptides can be resolved in a discontinuous sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) system using Tricine as the trailing ion. The seed proteins contain a number of charge species as determined by isoelectric focusing (IEF) in polyacrylamide gels. The peptides were focused in the basic region as determined by two-dimensional electrophoresis involving IEF and SDS-PAGE. The seed peptides have the unique property of being resistant to the action of silver nitrate, a sensitive reagent commonly used to stain proteins. The seed contains haemagglutinating activity which is inhibited by galactose.
Kabir
S
S
Academic Research and Information Management, Stockholm, Sweden.
D000596:Amino Acids / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006386:Hemagglutination Tests; D010455:Peptides / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D010946:Plants, Medicinal / Q000378:metabolism*; D012639:Seeds / Q000378:metabolism*
D000596:Amino Acids; D010455:Peptides; D010940:Plant Proteins