title: Intercellular and vascular cell adhesion molecule levels in endoscopic and open saphenous vein harvesting for coronary artery bypass surgery.
abstract: BACKGROUND
  Numbers of intercellular and vascular cell adhesion molecules (ICAM and VCAM) and major ligands on endothelial cells for adherence of activated polymorphnuclear leukocytes, macrophages, and lymphoid cells increase in many inflammatory disorders and after trauma to different tissues.
  
  
  METHODS
  Samples of human saphenous veins were harvested from 90 randomly selected patients who underwent coronary artery bypass graft (CABG) surgery, utilizing two different techniques (open and endoscopic). Endothelial cells were collected from the vein samples and cultured for 72 hours. Pre- and postoperative sera, in addition to the supernatants from the cultures, were analyzed for ICAM-1 and VCAM-1 using enzyme-linked immunosorbent assay.
  
  
  RESULTS
  Mean preoperative levels of ICAM-1 and VCAM-1 (0.95 +/- 0.58 ng/mL and 1.81 +/- 1.03 ng/mL, respectively) did not differ significantly from that of postoperative sera (0.98 +/- 0.451 ng/mL and 1.74 +/- 1.05 ng/mL, respectively) (p = 0.77 and p = 0.73, respectively). Mean ICAM-1 and VCAM-1 levels in endothelial cell culture supernatants did not differ significantly between the endoscopic (0.16 +/- 0.05 ng/mL and 0.23 +/- 0.10 ng/mL, respectively) and the open method (0.18 +/- 0.08 ng/mL and 0.30 +/- 0.27 ng/mL, respectively) (p = 0.19 and 0.13, respectively).
  
  
  CONCLUSIONS
  Our findings indicate that endoscopic and open saphenectomies are technically comparable in their effects on ICAM-1 and VCAM-1 synthesis during saphenous vein harvesting for CABG. We recommend the endoscopic method for its low morbidity and earlier hospital discharge.
authors.affiliation: Department of Surgery, Surgical Research Office, Maimonides & Lutheran Medical Center Research Institute, Brooklyn, New York 11220, USA.
authors.forename: S J
authors.initials: SJ
authors.lastname: Alrawi
authors.forename: M
authors.initials: M
authors.lastname: Samee
authors.forename: R
authors.initials: R
authors.lastname: Raju
authors.forename: D
authors.initials: D
authors.lastname: Shirazian
authors.forename: A J
authors.initials: AJ
authors.lastname: Acinapura
authors.forename: J N
authors.initials: JN
authors.lastname: Cunningham
mesh_terms: D017546:Angioscopy / Q000379:methods*; D015415:Biomarkers / Q000378:metabolism; D018929:Cell Culture Techniques; D001026:Coronary Artery Bypass / Q000379:methods*; D003327:Coronary Disease / Q000601:surgery*; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D012501:Saphenous Vein / Q000166:cytology / Q000378:metabolism / Q000637:transplantation*; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1