Generation of deletion and point mutations with one primer in a single cloning step.
Makarova
O
O
University of Michigan Medical School, Ann Arbor, USA.
Kamberov
E
E
Margolis
B
B
D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D017931:DNA Primers / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics; D016297:Mutagenesis, Site-Directed; D010957:Plasmids / Q000235:genetics; D017354:Point Mutation / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011933:Reagent Kits, Diagnostic; D017384:Sequence Deletion / Q000235:genetics*
D017931:DNA Primers; D011933:Reagent Kits, Diagnostic; D004262:DNA Restriction Enzymes