Polyclonal hematopoiesis with variable telomere shortening in human long-term allogeneic marrow graft recipients.
Donor-derived hematopoiesis was assessed in 17 patients who received allogeneic marrow grafts from HLA-matched siblings between 1971 and 1980. Complete blood counts were normal or near normal in all patients except one. Chimerism analyses, using either dual-color XY-chromosome fluorescence in situ hybridization (FISH) or analysis of variable number tandem repeat loci, indicated that 15 out of 16 patients had greater than 97% donor-derived hematopoiesis, whereas 1 patient had indeterminate chimerism. All 12 recipients of grafts from female donors exhibited polyclonal hematopoiesis by X-linked clonal analysis with the use of molecular probes. Of the 17 recipients, 9 exhibited a less than 1.0-kilobase shortening of granulocyte telomere length compared with their respective donors, according to terminal restriction fragment analysis or flow-FISH with a fluorescein-labeled peptide nucleic acid probe. These data suggest that under standard transplantation conditions, the stem cell proliferative potential is not compromised during hematopoietic reconstitution. (Blood. 2000;96:3991-3994)
Mathioudakis
G
G
Fred Hutchinson Cancer Research Center, Seattle, WA 98109-1024, USA. gmathiou@fhcrc.org
Storb
R
R
McSweeney
P A
PA
Torok-Storb
B
B
Lansdorp
P M
PM
Brümmendorf
T H
TH
Gass
M J
MJ
Bryant
E M
EM
Storek
J
J
Flowers
M E
ME
Gooley
T
T
Nash
R A
RA
D000293:Adolescent; D000328:Adult; D016026:Bone Marrow Transplantation / Q000592:standards; D002455:Cell Division / Q000502:physiology; D002648:Child; D002675:Child, Preschool; D005260:Female; D005500:Follow-Up Studies; D006098:Granulocytes / Q000648:ultrastructure; D006410:Hematopoiesis / Q000502:physiology*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D009679:Nuclear Family; D012150:Polymorphism, Restriction Fragment Length; D012737:Sex Factors; D016615:Telomere / Q000648:ultrastructure*; D018183:Transplantation Chimera; D014184:Transplantation, Homologous; D014960:X Chromosome / Q000648:ultrastructure