High-performance liquid chromatographic separation and measurement of various biogenic compounds possibly involved in the pathomechanism of Parkinson's disease.
The work presented here describes an optimised, reversed-phase high-performance liquid chromatographic (RP-HPLC) method for separating 46 biogenic compounds, which, as neurotoxins or as their precursors or derivatives, may be relevant in the pathomechanism of Parkinson's disease. In some cases, the physico-chemical properties of these substances are very similar, in other cases they differ greatly. In order to facilitate their detection in one chromatographic run, ion-pair chromatography was uniquely combined with a gradient elution. A diode array or a dual wavelength detector was used in combination with a fluorescence detector to verify the identity of the compounds.
Pagel
P
P
Abteilung Pharmakologie und Toxikologie der Universität Ulm, Germany.
Blome
J
J
Wolf
H U
HU
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007546:Isoquinolines / Q000097:blood / Q000302:isolation & purification*; D009498:Neurotoxins / Q000097:blood / Q000302:isolation & purification*; D010300:Parkinson Disease / Q000097:blood* / Q000503:physiopathology; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D007546:Isoquinolines; D009498:Neurotoxins