Local injection of IFN-gamma reduces the number of myofibroblasts and the collagen content in palatal wounds.
Wound contraction and scar formation after cleft palate surgery impair maxillary growth and dentoalveolar development. Since myofibroblast numbers and scar formation are reduced by interferon-gamma (IFN-gamma) in the healing of dermal wounds, the hypothesis was tested that local administration of IFN-gamma reduces the numbers of myofibroblasts and the elevated amount of collagen during palatal mucoperiosteal wound healing. Standardized mucoperiosteal excision wounds were made in the palatal mucoperiosteum of young rats. Either IFN-gamma or vehicle alone (sham group) was repeatedly injected into the wound site between 4 and 29 days post-wounding. The results were compared with unmanipulated control wounds. Samples of wound tissue were prepared for biochemical and microscopic analysis. The hydroxyproline, sulfated glycosaminoglycan and DNA contents of the wound tissues were analyzed biochemically. The degree of re-epithelialization, tissue thickness, the numbers of myofibroblasts, and the amounts of elastin and collagen types I and III were evaluated on histological sections. Injection of vehicle alone affected almost all healing parameters, compared with the controls, and delayed the wound-healing process. IFN-gamma stimulated re-epithelialization and decreased the numbers of myofibroblasts when compared with vehicle-treated wounds. It also decreased the hydroxyproline and glycosaminoglycan contents of 60-day-old wound tissue, but the histological characteristics of scar tissue persisted. Therefore, IFN-gamma is able to reduce the numbers of myofibroblasts and the collagen content of scar tissue after palatal wound healing. It may be a promising pharmaceutical agent for the reduction of wound contraction and scarring after cleft palate surgery.
Cornelissen
A M
AM
Department of Orthodontics and Oral Biology, College of Dental Science, University of Nijmegen, The Netherlands. orthodontics@dent.kun.nl
Maltha
J C
JC
Von den Hoff
J W
JW
Kuijpers-Jagtman
A M
AM
D008841:Actin Cytoskeleton / Q000187:drug effects; D000704:Analysis of Variance; D000818:Animals; D016009:Chi-Square Distribution; D002921:Cicatrix / Q000517:prevention & control; D003094:Collagen / Q000096:biosynthesis / Q000187:drug effects; D003286:Contracture / Q000517:prevention & control*; D005347:Fibroblasts / Q000187:drug effects*; D006025:Glycosaminoglycans / Q000096:biosynthesis; D006909:Hydroxyproline / Q000096:biosynthesis; D007371:Interferon-gamma / Q000494:pharmacology* / Q000627:therapeutic use*; D008297:Male; D009061:Mouth Mucosa / Q000293:injuries / Q000601:surgery; D010159:Palate / Q000293:injuries / Q000601:surgery; D051381:Rats; D017208:Rats, Wistar; D018709:Statistics, Nonparametric; D014945:Wound Healing / Q000187:drug effects*
D006025:Glycosaminoglycans; D007371:Interferon-gamma; D003094:Collagen; D006909:Hydroxyproline