Comparison of viral genomic RNA sorting mechanisms in human immunodeficiency virus type 1 (HIV-1), HIV-2, and Moloney murine leukemia virus.
Genomic RNA sorting between translation and packaging was examined for human immunodeficiency virus type 1 (HIV-1) and HIV-2 using actinomycin D and leptomycin B treatment. Both viruses behaved differently from a simple retrovirus under actinomycin D treatment. With leptomycin B, the lack of apparent functional separation between translation and packaging functions in lentiviruses was confirmed. HIV-2 RNA levels were more stable, but reverse transcriptase production declined similarly to HIV-1.
Dorman
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University of Cambridge Department of Medicine, Addenbrooke's Hospital, Cambridge CB2 2QQ, United Kingdom.
Lever
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A
D003609:Dactinomycin / Q000494:pharmacology; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology; D015497:HIV-1 / Q000502:physiology*; D015498:HIV-2 / Q000502:physiology*; D008979:Moloney murine leukemia virus / Q000502:physiology*; D012367:RNA, Viral / Q000502:physiology*; D019065:Virus Assembly
D005231:Fatty Acids, Unsaturated; D012367:RNA, Viral; D003609:Dactinomycin; C038753:leptomycin B