Small molecule-based chemical affinity system for the purification of proteins.
A new chemical affinity system is described for the purification of proteins. The Linx Affinity Purification System enables researchers to quickly and easily bind a protein ligand to a chromatographic matrix and use the resulting affinity resin to purify a second protein from crude mixtures. The entire process takes approximately 2 h.
Bergseid
M
M
Invitrogen, Carlsbad, CA, USA.
Baytan
A R
AR
Wiley
J P
JP
Ankener
W M
WM
Stolowitz
M L
ML
Hughes
K A
KA
Chesnut
J D
JD
D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D000906:Antibodies / Q000276:immunology / Q000302:isolation & purification; D001897:Boronic Acids / Q000737:chemistry; D002021:Buffers; D002457:Cell Extracts / Q000737:chemistry; D002846:Chromatography, Affinity / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D004800:Enzymes, Immobilized / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D006863:Hydrogen-Ion Concentration; D006877:Hydroxamic Acids / Q000737:chemistry; D007106:Immune Sera / Q000737:chemistry; D015394:Molecular Structure; D008970:Molecular Weight; D011485:Protein Binding / Q000187:drug effects; D011506:Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D012259:Ribonuclease, Pancreatic / Q000276:immunology / Q000378:metabolism; D012457:Salicylamides / Q000737:chemistry; D012685:Sepharose / Q000378:metabolism; D013242:Sterilization
D000890:Anti-Infective Agents; D000906:Antibodies; D001897:Boronic Acids; D002021:Buffers; D002457:Cell Extracts; D004800:Enzymes, Immobilized; D000939:Epitopes; D006877:Hydroxamic Acids; D007106:Immune Sera; D011506:Proteins; D011993:Recombinant Fusion Proteins; D012457:Salicylamides; C005703:salicylhydroxamic acid; D012685:Sepharose; D012259:Ribonuclease, Pancreatic