[Hepatitis C virus contamination of endoscopes and biopsy forceps].
BACKGROUND
Procedures such as digestive endoscopy may explain some unclear contaminations by HCV.


OBJECTIVE
The aims of this study were to detect HCV genome on endoscopes and biopsy-forceps used in patients with known chronic HCV infection and to determine its presence in their gastric juice and saliva.


METHODS
A gastroscopy with antral biopsies was performed in 48 patients with non-treated replicative chronic hepatitis C. Samples were obtained after pushing 10 mL of sterile water through the biopsy-suction channel and after immersing the brush used to clean this channel. The biopsy-forceps were also immersed and their tips brushed in 10 mL of sterile water. This sampling technique was repeated three times: immediately after the endoscopic procedure (T0), after washing with a detergent (T1) and after immersion for 20 minutes in a 2% glutaraldehyde solution (T2). The HCV genome was detected by polymerase chain reaction (PCR, Amplicor - Roche Diagnostics Systems). For the last 15 patients, samples of gastric juice and saliva were obtained before antral biopsies and used to detect HCV genome.


RESULTS
HCV genome was detected in the biopsy-suction channel in 13 cases (27%) at T0 and in one case (2%) at T1. It was undetectable after completion of the disinfection procedure (T2). Three biopsy-forceps (6%) were PCR positive immediately after the endoscopy but none at T1 and T2. HCV genome was found in the gastric juice in three cases. In all of them, it was also found at T0 in the biopsy-suction channel but not on the biopsy-forceps. When saliva contained HCV genome (4 cases), it was present in the biopsy-suction channel in only one case. In this case, the gastric juice was also PCR positive.


CONCLUSIONS
HCV genome is detected in 27% of cases in the biopsy-suction channel after an endoscopic procedure performed on patients with chronic HCV infection. The biopsy-forceps are PCR positive in 6% of cases. The infected gastric juice may play a role in the contamination of the endoscopes. The complete disinfection procedure seems effective to eliminate HCV.
Bécheur
H
H
Service d'Hépato-Gastroentérologie, Centre Hospitalier de Versailles, Le Chesnay.
Harzic
M
M
Colardelle
P
P
Deny
P
P
Coste
T
T
Dubeaux
B
B
Chochon
M
M
Roussin-Bretagne
S
S
Doll
J
J
Andrieu
J
J
D001706:Biopsy / Q000295:instrumentation*; D019723:Endoscopes; D004866:Equipment Contamination; D005750:Gastric Juice / Q000821:virology; D016174:Hepacivirus / Q000235:genetics / Q000302:isolation & purification*; D019698:Hepatitis C, Chronic / Q000821:virology; D006801:Humans; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis; D012463:Saliva / Q000821:virology; D013525:Surgical Instruments
D012367:RNA, Viral