Use of a real-time, coupled assay to measure the ATPase activity of DNA topoisomerase II.
Lindsley
J E
JE
Department of Biochemistry, University of Utah, School of Medicine, Salt Lake City, USA.
D000251:Adenosine Triphosphatases / Q000032:analysis / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D002021:Buffers; D004250:DNA Topoisomerases, Type II / Q000032:analysis* / Q000378:metabolism*; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D010728:Phosphoenolpyruvate / Q000378:metabolism; D011770:Pyruvate Kinase / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013053:Spectrophotometry / Q000379:methods
D002021:Buffers; D009243:NAD; D010728:Phosphoenolpyruvate; D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase; D011770:Pyruvate Kinase; D000251:Adenosine Triphosphatases; D004250:DNA Topoisomerases, Type II