Effects of transient ethanol exposure on the incorporation of [(3)H]ethanolamine into plasmalogen in the differentiating CG-4 oligodendrocyte cell line.
We investigated the potential teratogenic effects of ethanol (EtOH) on myelination by monitoring its effects on the labeling of the myelin-typical lipid, ethanolamine plasmalogen (EPl), in the CG-4 cell line of differentiating oligodendrocytes (OLGs). On 5 different days during the first 8 days of OLG development, cells were labeled for 24 hr with [(3)H]ethanolamine to label EPl and diacyl-ethanolamine phosphoglycerols (diacyl-EPG), and the amount of labeled lipid expressed on each day was determined in the presence and absence of 25-120 mM EtOH. At early stages of development, a lower amount of [(3)H]EPl per cell was found in cells exposed to EtOH. The ratio of [(3)H]EPl to [(3)H]diacyl-EPG in cells exposed to 25, 50, or 120 mM EtOH was decreased by 50% after 4 days of differentiation compared with that in control cells. By adding or withdrawing EtOH at specific days of differentiation, we showed that EtOH inhibited the increased labeling of EPl if it was present for the first 48 hr of differentiation, and subsequent withdrawal failed to relieve the inhibition. Addition of EtOH anytime after the first day of differentiation did not inhibit the increased labeling of EPl. The results show that the increased labeling of EPl in differentiating OLGs resulted from an EtOH-sensitive, developmentally programmed, transient process active only during the first 2 days of differentiation.
Bichenkov
E
E
Department of Pathology, Anatomy, and Cell Biology, Medical College of Thomas Jefferson University, 19107, USA, Philadelphia, PA, USA.
Ellingson
J S
JS
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D002492:Central Nervous System Depressants / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology*; D019856:Ethanolamine / Q000378:metabolism*; D009836:Oligodendroglia / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010955:Plasmalogens / Q000378:metabolism*; D051381:Rats; D013997:Time Factors; D014316:Tritium
D002492:Central Nervous System Depressants; D010955:Plasmalogens; D014316:Tritium; D000431:Ethanol; D019856:Ethanolamine