Influence of aerobic oxidation of mouse erythrocytes on their recognition by macrophages.
Membrane protein modification can change cell surface properties which can be correlated with altered macrophage-erythrocyte interactions. Mouse erythrocytes were incubated in phosphate buffer for different times to induce protein modification. Mouse erythrocyte membrane changes were analyzed by infrared analyses and gel electrophoresis. Proteolytic digestion of membrane proteins was observed. After 22 hours preliminary incubation, the number of erythrocytes adhering to a monolayer of macrophages reached a maximum, the majority of which had not been phagocytosed. Most of the erythrocytes incubated for 40 hours underwent phagocytosis after adhesion to the macrophages.
Olmos
G
G
Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá, Alcalá de Henares, Madrid, Spain.
Lotero
L A
LA
Herráez
A
A
Alvarez
F J
FJ
Murciano
J C
JC
Tejedor
M C
MC
Díez
J C
JC
D000818:Animals; D002021:Buffers; D002448:Cell Adhesion / Q000187:drug effects; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D002857:Chromium / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000378:metabolism; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D008264:Macrophages / Q000166:cytology / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D016877:Oxidants / Q000494:pharmacology*; D010587:Phagocytosis / Q000187:drug effects; D010710:Phosphates / Q000494:pharmacology; D013055:Spectrophotometry, Infrared
D002021:Buffers; D008565:Membrane Proteins; D016877:Oxidants; D010710:Phosphates; D002857:Chromium; D010450:Endopeptidases