Meiotic nondisjunction of chromosomes 1, 17, 18, X, and Y in men more than 80 years of Age.
In order to evaluate a possible paternal age effect, testicular sperm cells from three men aged 81, 82, and 83 yr were analyzed by two-color- and three-color-fluorescence in situ hybridization for disomy rates of chromosomes 1, 17, 18, X, and Y as well as for diploidy frequencies. A minimum of 1500 sperm cells per donor and probe was evaluated due to the low number of spermatozoa in the preparations. Diploidy and disomy frequencies were in the same range as found in men aged <30 yr, a slight increase only being noticed for XY nuclei.
Guttenbach
M
M
Institut für Humangenetik, Universität Würzburg, Biozentrum, Am Hubland, 97074 Würzburg, Germany.
Köhn
F M
FM
Engel
W
W
Schmid
M
M
D000368:Aged; D000369:Aged, 80 and over / Q000502:physiology*; D002875:Chromosomes / Q000235:genetics*; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics; D002887:Chromosomes, Human, Pair 18 / Q000235:genetics; D004247:DNA / Q000032:analysis; D004171:Diploidy; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008540:Meiosis / Q000235:genetics*; D009630:Nondisjunction, Genetic; D013094:Spermatozoa / Q000648:ultrastructure; D013737:Testis / Q000737:chemistry / Q000648:ultrastructure; D014960:X Chromosome / Q000235:genetics; D014998:Y Chromosome / Q000235:genetics
D004247:DNA