title: Production in mammalian cells of chimeric human/sea urchin procollagen molecules displaying distinct versions of the minor triple helix.
abstract: One of the mechanisms involved in the regulation of the fibril diameter is the retention of the N-propeptide. In sea urchin embryo, thin collagen fibrils harbor numerous extensions at their surface, which we have suggested correspond to the large N-propeptide of the 2alpha collagen chain. To investigate the function of the N-propeptide during fibrillogenesis, we engineered constructs coding for the globular region of the 2alpha N-propeptide. To obtain homotrimeric molecules, the N-telopeptide, the central triple helix and the C-propeptide of the 2alpha chain were replaced by human domains of the proalpha1(I) chain. A single restriction site allowed insertion of distinct versions of the minor triple helix of the N-propeptide. Several human cell lines were transfected, and with one of them we were able to produce intact homotrimeric procollagen molecules. Rotary shadowing of these purified molecules indicates the presence of three large 2alpha N-propeptides that are similar to the extensions present at the surface of the sea urchin thin fibrils. This cassette-vector will be useful in determining the respective contributions of the globular and minor triple helical domains of the N-propeptide in the regulation of fibril diameter.
authors.affiliation: Institut de Biologie et Chimie des Protéines, CNRS, Unité Mixte de Recherche Université Claude Bernard, passage du Vercors, 69367 Lyon cedex 07, France.
authors.forename: C
authors.initials: C
authors.lastname: Cluzel
authors.forename: C
authors.initials: C
authors.lastname: Lethias
authors.forename: R
authors.initials: R
authors.lastname: Garrone
authors.forename: J Y
authors.initials: JY
authors.lastname: Exposito
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D006801:Humans; D008854:Microscopy, Electron; D011347:Procollagen / Q000096:biosynthesis* / Q000737:chemistry / Q000648:ultrastructure; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*; D012617:Sea Urchins; D014407:Tumor Cells, Cultured
chemical_list: D017931:DNA Primers; D011347:Procollagen; D011993:Recombinant Fusion Proteins