Interaction of the herpes simplex virus type 1 packaging protein UL15 with full-length and deleted forms of the UL28 protein.
The UL15 and UL28 proteins of herpes simplex virus type 1 are both required for the packaging of replicated viral DNA into the viral capsid. We have expressed UL28 and a functional epitope-tagged form of UL15 in mammalian and insect cells. Immunoprecipitation experiments confirmed that the two proteins can interact. In agreement with previous results, UL15, when expressed alone, entered the nucleus but UL28 remained cytoplasmic. When co-expressed the two proteins co-localized in the nucleus. Six UL28 deletion mutants were constructed and similarly analysed. The results obtained by immunoprecipitation and immunofluorescence were consistent and demonstrate that at least two separate regions of the UL28 polypeptide chain have the ability to interact with UL15. Surprisingly, three of the mutants prevented the UL15 protein from localizing to the cell nucleus, and these were not functional in a transient DNA packaging assay. Of the three UL28 mutant proteins that entered the nucleus with UL15, one containing an internal deletion of 13 amino acids was able to complement a UL28 null mutant in both DNA packaging and virus yield assays, demonstrating that this region of the protein is not essential for function. In addition to interacting with the UL28 protein we also demonstrated that UL15 molecules can interact with each other, and that sequences within the second exon contribute to this interaction.
Abbotts
Adrian P
AP
MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK1.
Preston
Valerie G
VG
MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK1.
Hughes
Michelle
M
MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK1.
Patel
Arvind H
AH
MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK1.
Stow
Nigel D
ND
MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK1.
D021581:Active Transport, Cell Nucleus; D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D002522:Chlorocebus aethiops; D006224:Cricetinae; D005091:Exons / Q000235:genetics; D005455:Fluorescent Antibody Technique; D005816:Genetic Complementation Test; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000502:physiology; D011233:Precipitin Tests; D011485:Protein Binding; D017384:Sequence Deletion / Q000235:genetics*; D018411:Spodoptera; D014162:Transfection; D014709:Vero Cells; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D019065:Virus Assembly
D014764:Viral Proteins; C069185:DNA cleavage and packaging proteins, Herpesvirus