title: Structure of the TPR domain of p67phox in complex with Rac.GTP.
abstract: p67phox is an essential part of the NADPH oxidase, a multiprotein enzyme complex that produces superoxide ions in response to microbial infection. Binding of the small GTPase Rac to p67phox is a key step in the assembly of the active enzyme complex. The structure of Rac.GTP bound to the N-terminal TPR (tetratricopeptide repeat) domain of p67phox reveals a novel mode of Rho family/effector interaction and explains the basis of GTPase specificity. Complex formation is largely mediated by an insertion between two TPR motifs, suggesting an unsuspected versatility of TPR domains in target recognition and in their more general role as scaffolds for the assembly of multiprotein complexes.
authors.affiliation: Division of Protein Structure, National Institute for Medical Research, London, United Kingdom.
authors.forename: K
authors.initials: K
authors.lastname: Lapouge
authors.forename: S J
authors.initials: SJ
authors.lastname: Smith
authors.forename: P A
authors.initials: PA
authors.lastname: Walker
authors.forename: S J
authors.initials: SJ
authors.lastname: Gamblin
authors.forename: S J
authors.initials: SJ
authors.lastname: Smerdon
authors.forename: K
authors.initials: K
authors.lastname: Rittinger
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002151:Calorimetry; D006160:Guanosine Triphosphate / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009252:NADPH Dehydrogenase / Q000737:chemistry / Q000378:metabolism; D010750:Phosphoproteins / Q000737:chemistry* / Q000378:metabolism*; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D020449:Repetitive Sequences, Amino Acid; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013816:Thermodynamics; D020744:rac GTP-Binding Proteins / Q000737:chemistry* / Q000378:metabolism*; D000097766:RAC2 GTP-Binding Protein
chemical_list: D006160:Guanosine Triphosphate; D009252:NADPH Dehydrogenase; D010750:Phosphoproteins; D011994:Recombinant Proteins; D020744:rac GTP-Binding Proteins; D000097766:RAC2 GTP-Binding Protein; C079789:neutrophil cytosol factor 67K