Correlation between profile of circulating mononuclear cells and clinical manifestations in patients with pemphigus vulgaris.
Phenotypes of 38 samples of mononuclear (PBMC) cells from 11 different patients with pemphigus vulgaris (PV) at different stages of the disease were explored looking for a possible relationship between cell immunity, mucocutaneous or mucosal lesion intensity and capacity of serum autoantibodies to elicit the disease in mice. PBMC from 5 patients with mucocutaneous lesions and sera with IgG capable of inducing the disease in neonatal mice had a high proportion of mature monocytes with CD14low DRhigh, and co-expressing CD16 and CD11b. In addition, a high proportion of CD19+CD5+ activated B cells and a very low proportion of naive CD4+CD45RA+ and CD8+CD11b+ T lymphocytes was observed. Monocytes from these patients expressed inducible nitric oxide synthase (iNOS). In contrast, PBMC from 6 patients, with lesions restricted to mucosal membranes and IgG lacking the capacity to induce the disease in mice, contained a high proportion of CD14high DRlow co-expressing CD16 circulating macrophages, CD8+CD11b+ T cells, and a low proportion of activated B lymphocytes. The results suggest a possible association between proportion of different antigen presenting cells (monocytes with high HLA-DR and low CD14 expression and activated B lymphocytes, or differentiated monocytes/macrophages), type of PV and capacity of serum autoantibodies to elicit the disease in mice.
González
A
A
Department of Biochemistry, University of Navarra, Pamplona, Spain.
España
A
A
López-Zabalza
M J
MJ
Pelacho
B
B
Sánchez-Carpintero
I
I
Santiago
E
E
López-Moratalla
N
N
D000051:Acantholysis / Q000209:etiology / Q000276:immunology / Q000473:pathology; D000818:Animals; D000831:Animals, Newborn; D001323:Autoantibodies / Q000008:administration & dosage / Q000097:blood; D004195:Disease Models, Animal; D006684:HLA-DR Antigens / Q000097:blood; D006801:Humans; D007116:Immunization, Passive; D007074:Immunoglobulin G / Q000008:administration & dosage / Q000097:blood; D016130:Immunophenotyping; D007963:Leukocytes, Mononuclear / Q000201:enzymology / Q000276:immunology*; D018950:Lipopolysaccharide Receptors / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000145:classification / Q000201:enzymology / Q000276:immunology; D019001:Nitric Oxide Synthase / Q000097:blood; D052247:Nitric Oxide Synthase Type II; D010392:Pemphigus / Q000201:enzymology / Q000276:immunology* / Q000473:pathology
D001323:Autoantibodies; D006684:HLA-DR Antigens; D007074:Immunoglobulin G; D018950:Lipopolysaccharide Receptors; C496319:NOS2 protein, human; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse