Rapid staining of proteins on polyacrylamide gels and nitrocellulose membranes using a mixture of fluorescent dyes.
The present work describes a novel, fluorescence-based method for staining proteins on SDS-PAGE and membrane(s). In this method, proteins are stained using a mixed-dye (sulfo-rhodamine B and 1-anilino-8-naphthalene sulfonic acid (NH(4)(+))) solution. The mixed-dye staining protocol can detect proteins up to a concentration of 15 ng. This method is generally applicable to all proteins and is more sensitive than the conventional Coomassie blue method. The staining method is rapid and efficient. Staining-destaining of proteins using the mixed-dye protocol takes less than half an hour. Another interesting feature of the staining protocol described here is the applicability to the staining of proteins on nitrocellulose membranes.
Ganesh
G
G
Department of Chemistry, National Tsing Hua University, Hsinchu, Taiwan.
Kumar
T K
TK
Pandian
S T
ST
Yu
C
C
D000817:Anilino Naphthalenesulfonates / Q000378:metabolism; D000818:Animals; D002256:Carbonic Anhydrases / Q000032:analysis / Q000378:metabolism; D003101:Collodion / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005346:Fibroblast Growth Factors / Q000032:analysis / Q000378:metabolism; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000378:metabolism*; D011506:Proteins / Q000032:analysis* / Q000378:metabolism; D012235:Rhodamines / Q000378:metabolism; D012394:Rosaniline Dyes / Q000378:metabolism; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods*; D013997:Time Factors
D000817:Anilino Naphthalenesulfonates; D005456:Fluorescent Dyes; D011506:Proteins; D012235:Rhodamines; D012394:Rosaniline Dyes; D005346:Fibroblast Growth Factors; C027132:1-anilino-8-naphthalenesulfonate; C048139:Coomassie blue; D003101:Collodion; D002256:Carbonic Anhydrases