title: Collagen fiber reorganization in the rat ventral prostate following androgen deprivation: a possible role for smooth muscle cells.
abstract: BACKGROUND
  Stroma plays an essential role in glandular function in different systems. In the prostate, it is responsible for the development and maintenance of the differentiated state of the epithelium. The marked reduction in the epithelial compartment of the prostate gland following castration is followed by a similarly important reorganization of the stroma. In this work, we characterized the reorganization of collagen fibers in the ventral prostate of castrated rats.
  
  
  METHODS
  Histochemical tests and immunohistochemistry for type I and III collagens plus confocal microscopy of triple-labeled (collagen III, actin, and DNA) tissue sections were employed.
  
  
  RESULTS
  We showed that collagen fibers are composed of type I and type III collagens and that they are progressively concentrated around the epithelial structures (ducts and acini) and become increasingly undulated and folded. Double-labeling of collagen fibers and F-actin demonstrated that smooth muscle cells (SMC) are intimately associated with collagen fibers.
  
  
  CONCLUSIONS
  The results demonstrated a marked reorganization of the collagen fibers, and suggest an active role of the SMC in the reorganization of the fibrillar components of the stroma.
authors.affiliation: Department of Cell Biology, UNICAMP, Campinas, São Paulo, Brazil.
authors.forename: P S
authors.initials: PS
authors.lastname: Vilamaior
authors.forename: S L
authors.initials: SL
authors.lastname: Felisbino
authors.forename: S R
authors.initials: SR
authors.lastname: Taboga
authors.forename: H F
authors.initials: HF
authors.lastname: Carvalho
mesh_terms: D000818:Animals; D002369:Castration; D002454:Cell Differentiation; D003094:Collagen / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure*; D007150:Immunohistochemistry; D008297:Male; D009130:Muscle, Smooth / Q000166:cytology / Q000502:physiology*; D011467:Prostate / Q000187:drug effects / Q000648:ultrastructure*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D003094:Collagen