Oligodendrocyte precursor migration and differentiation: combined effects of PSA residues, growth factors, and substrates.
Using the oligosphere strategy (V. Avellana-Adalid et al., 1996, J. Neurosci. Res. 45, 558-570), we compared the migratory behavior of oligodendrocyte preprogenitors (OPP) that expressed the polysialylated form of the neural cell adhesion molecule (PSA-NCAM) and of GD3-positive oligodendrocyte progenitors (OP). To study the role of PSA in OPP migration, we used endoneuraminidase-N, which specifically cleaves PSA from NCAM. Kinetic data showed that (i) migration velocity decreased with time and was favored on polyornithine compared to Matrigel; (ii) cells emerging from spheres enriched in PSA-NCAM+ OPP migrated farther than those from spheres enriched in GD3+ OP, their migration being enhanced by the addition of growth factors; (iii) removal of PSA from NCAM moderately reduced OPP migration and induced their differentiation in GD3+ OP and GFAP+ astrocytes; (iv) blocking integrins reduced their migration, suggesting an alternative mechanism of migration. Altogether these data illustrate that motility and differentiation of OPP involve the combinatorial action of PSA-NCAM, molecules of the ECM and their receptors, and growth factors.
Decker
L
L
Laboratoire des Pathologies de la Myéline, Institut National de la Santé et de la Recherche Médicale, Paris, France.
Avellana-Adalid
V
V
Nait-Oumesmar
B
B
Durbec
P
P
Baron-Van Evercooren
A
A
D000818:Animals; D000831:Animals, Newborn; D001672:Biocompatible Materials / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D048429:Cell Size / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology*; D004338:Drug Combinations; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006026:Glycoside Hydrolases / Q000494:pharmacology; D007797:Laminin / Q000494:pharmacology*; D039842:Neural Cell Adhesion Molecule L1; D019006:Neural Cell Adhesion Molecules / Q000032:analysis / Q000378:metabolism*; D009836:Oligodendroglia / Q000166:cytology*; D010455:Peptides / Q000494:pharmacology; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D011509:Proteoglycans / Q000494:pharmacology*; D051381:Rats; D012794:Sialic Acids / Q000032:analysis / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology*; D013501:Surface-Active Agents / Q000494:pharmacology
D001672:Biocompatible Materials; D004338:Drug Combinations; D005904:Glial Fibrillary Acidic Protein; D007797:Laminin; D039842:Neural Cell Adhesion Molecule L1; D019006:Neural Cell Adhesion Molecules; D010455:Peptides; D010982:Platelet-Derived Growth Factor; D011509:Proteoglycans; D012794:Sialic Acids; D013501:Surface-Active Agents; C075278:platelet-derived growth factor A; C103469:polysialyl neural cell adhesion molecule; D016222:Fibroblast Growth Factor 2; C059485:matrigel; C008973:polyornithine; D003094:Collagen; D006026:Glycoside Hydrolases; C090534:endo-alpha-sialidase