title: Effect of hepatic denervation on the counterregulatory response to insulin-induced hypoglycemia in the dog.
abstract: Our aim was to determine whether complete hepatic denervation would affect the hormonal response to insulin-induced hypoglycemia in dogs. Two weeks before study, dogs underwent either hepatic denervation (DN) or sham denervation (CONT). In addition, all dogs had hollow steel coils placed around their vagus nerves. The CONT dogs were used for a single study in which their coils were perfused with 37 degrees C ethanol. The DN dogs were used for two studies in a random manner, one in which their coils were perfused with -20 degrees C ethanol (DN + COOL) and one in which they were perfused with 37 degrees C ethanol (DN). Insulin was infused to create hypoglycemia (51 +/- 3 mg/dl). In response to hypoglycemia in CONT, glucagon, cortisol, epinephrine, norepinephrine, pancreatic polypeptide, glycerol, and hepatic glucose production increased significantly. DN alone had no inhibitory effect on any hormonal or metabolic counterregulatory response to hypoglycemia. Likewise, DN in combination with vagal cooling also had no inhibitory effect on any counterregulatory response except to reduce the arterial plasma pancreatic polypeptide response. These data suggest that afferent signaling from the liver is not required for the normal counterregulatory response to insulin-induced hypoglycemia.
authors.affiliation: Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA.
authors.forename: P A
authors.initials: PA
authors.lastname: Jackson
authors.forename: S
authors.initials: S
authors.lastname: Cardin
authors.forename: C S
authors.initials: CS
authors.lastname: Coffey
authors.forename: D W
authors.initials: DW
authors.lastname: Neal
authors.forename: E J
authors.initials: EJ
authors.lastname: Allen
authors.forename: A R
authors.initials: AR
authors.lastname: Penaloza
authors.forename: W L
authors.initials: WL
authors.lastname: Snead
authors.forename: A D
authors.initials: AD
authors.lastname: Cherrington
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000097:blood; D000409:Alanine / Q000097:blood; D000818:Animals; D001786:Blood Glucose / Q000096:biosynthesis / Q000378:metabolism; D003080:Cold Temperature; D003243:Consciousness; D004285:Dogs; D004837:Epinephrine / Q000097:blood; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D005934:Glucagon / Q000097:blood; D005990:Glycerol / Q000097:blood; D006339:Heart Rate / Q000502:physiology; D006854:Hydrocortisone / Q000097:blood; D007003:Hypoglycemia / Q000097:blood* / Q000139:chemically induced; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology*; D019344:Lactic Acid / Q000097:blood; D008099:Liver / Q000294:innervation* / Q000378:metabolism*; D008297:Male; D009638:Norepinephrine / Q000097:blood; D010191:Pancreatic Polypeptide / Q000097:blood; D017764:Parasympathectomy; D014630:Vagus Nerve / Q000502:physiology
chemical_list: D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D007004:Hypoglycemic Agents; D007328:Insulin; D019344:Lactic Acid; D010191:Pancreatic Polypeptide; D005934:Glucagon; D000409:Alanine; D005990:Glycerol; D020155:3-Hydroxybutyric Acid; D006854:Hydrocortisone; D009638:Norepinephrine; D004837:Epinephrine