title: Correlation between type II nitric oxide synthase and p53 expression in oral squamous cell carcinoma.
abstract: Prolonged nitric oxide (NO) production by the enzyme type II nitric oxide synthase (NOS2) has been implicated in angiogenesis and metastasis of human cancers. In animal models, wild-type p53 (but not mutant) protein results in down-regulation of NOS2 expression, which reduces both tumour growth and dissemination. In the current study, we aimed to find out whether a correlation was present in oral squamous cell carcinoma. Fifty-six cases of squamous cell carcinoma were assessed immunohistochemically using antibodies to NOS2 and p53 (clone DO-7). We also confirmed NOS2 protein expression in selected cases using immunoblotting. The results were correlated with clinicopathological findings. Statistical analysis showed a significant relationship between p53 and NOS2 expression (P= 0.001). No relationship was found between size of tumour or histological degree of differentiation, and NOS2 expression in the primary tumour.
authors.affiliation: BUPA Research Fellow, Maxillofacial Department, University of Portsmouth, Portsmouth, UK.
authors.forename: P A
authors.initials: PA
authors.lastname: Brennan
authors.forename: M
authors.initials: M
authors.lastname: Palacios-Callender
authors.forename: T
authors.initials: T
authors.lastname: Umar
authors.forename: D
authors.initials: D
authors.lastname: Hughes
authors.forename: A V
authors.initials: AV
authors.lastname: Spedding
authors.forename: G A
authors.initials: GA
authors.lastname: Zaki
authors.forename: J D
authors.initials: JD
authors.lastname: Langdon
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000201:enzymology*; D004790:Enzyme Induction; D006651:Histocytochemistry; D006801:Humans; D015151:Immunoblotting; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000201:enzymology*; D019001:Nitric Oxide Synthase / Q000096:biosynthesis*; D052247:Nitric Oxide Synthase Type II; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis* / Q000502:physiology
chemical_list: D016159:Tumor Suppressor Protein p53; C496319:NOS2 protein, human; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II