Crystallization and preliminary X-ray analysis of earthworm fibrinolytic enzyme component A from Eisenia fetida.
Earthworm fibrinolytic enzyme component A, a protein which functions both as a direct fibrinolytic enzyme and a plasminogen activator, was purified from the earthworm Eisenia fetida. Diffraction-quality single crystals of the protein were grown by the hanging-drop vapour-diffusion technique with ammonium sulfate as a precipitant. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 40.6, b = 127.5, c = 129.2 A and three molecules per asymmetric unit. The data set reached a resolution of 1.95 A.
Tang
Y
Y
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, Beijing 100101, People's Republic of China.
Zhang
J
J
Gui
L
L
Wu
C
C
Fan
R
R
Chang
W
W
Liang
D
D
D000818:Animals; D018360:Crystallography, X-Ray; D010450:Endopeptidases / Q000737:chemistry* / Q000302:isolation & purification; D009835:Oligochaeta / Q000201:enzymology*; D010960:Plasminogen Activators / Q000737:chemistry / Q000302:isolation & purification; D011487:Protein Conformation
D010450:Endopeptidases; D010960:Plasminogen Activators; C083421:lumbrokinase