Dynamic cell cycle kinetics in vitro and in vivo in myelodysplastic syndromes with special reference to the influence of hematopoietic growth factors.
We have investigated the effect of HGF in vivo and in vitro in MDS using a recently developed FCM assay involving the simultaneous measurement of cell surface antigens, DNA content, and BrdUrd or IodUrd incorporation. This allows for the determination of the dynamic cell kinetic parameters: LI, T(s), and T(pot) and we observed that in vitro HGF stimulation resulted in a significant decrease in mean T(pot) values from 6.6 to 3.5 days. Importantly, we demonstrated that in vivo GM-CSF administration to patients with RAEB resulted in a shortening of T(pot) within the 2 first weeks of GM-CSF treatment.
Holm
M
M
Department of Hematology, Immunhaematologisk Laboratorium, Arhus amtssygehus, Tage-Hansens Gade 2, Opgang 4A, DK 8000, Arhus, Denmark. andholm@post11.tele.dk
Hoyer
M
M
Jensen
I
I
Thomsen
M
M
Hokland
P
P
D000368:Aged; D015703:Antigens, CD; D001854:Bone Marrow Cells / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects*; D004247:DNA / Q000032:analysis; D005260:Female; D005434:Flow Cytometry; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology / Q000627:therapeutic use*; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology*; D006801:Humans; D016130:Immunophenotyping; D007963:Leukocytes, Mononuclear / Q000473:pathology; D019891:Leukopoiesis; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics / Q000473:pathology* / Q000628:therapy*; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D016298:Hematopoietic Cell Growth Factors; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D004247:DNA