Analysis of Cytoskeletal Proteins in Rat Selenium Cataract Using Two-dimensional Electrophoresis.
Background and Purpose: Previous studies used sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to characterize the modifications to lens proteins during opacification in the selenite model for cataract. To analyze the protein modifications in more detail, two dimensional electrophoresis (2 DE), which is more sensitive than 1 D electrophoresis, was used.Results: The results of 2 DE demonstrated rapid changes in cytoskeletal proteins including spectrin and vimentin at the earliest stages of opacification.Conclusion: The results suggested that the mechanism of opacification might involve changes in cytoskeletal proteins during the earliest stages of cataract formation.
Matsushima
H
Department of Ophthalmology, Dokkyo University School of Medicine, Dokkyo, Japan
Mukai
K
Obara
Y
Clark
JI