Adhesion of Human Breast Cancer Cells to Vascular Endothelium Mediated by Sialyl Lewis &supx; /E-selectin.
The adhesion molecules involved in the attachment of breast cancer cells to endothelial cells were investigated in vitro. All six human breast cancer cell lines expressed sialyl Lewis &supx; antigen(s-Le &supx;). Only two cell lines expressed sialyl Lewis&supa; antigen. A correlation was found between the degree of s-Le&supx; expression and the attachment of cancer cells to human umbilical vein endothelial cells (HUVECs) activated by IL-1beta. Monoclonal antibodies against s-Le &supx; or E-selectin inhibited this adhesion. These findings suggest that s-Le &supx; on the surface of cancer cells and E-selectin on the surface of endothelial cells play roles in adheision of breast cancer cells to vascular endothelium.
Narita
T
Laboratory of Experimental Pathology, Research Institute, Aichi Cancer Center, 1-1 Kanokoden, Chikusa-ku, Nagoya 464, Japan.
Kawasaki-Kimura
N
Matsuura
N
Funahashi
H
Kannagi
R