Evaluation of the genotoxic and cytochrome P450 monooxygenase-inhibitory potential of Dicuran on procaryotic and eucaryotic test systems.
The effect of the herbicide Dicuran 500 FL (formulated product) on the phenotypical and genotypical changes in procaryotic and eucaryotic organisms was investigated using short-term tests for detecting genotoxins. Since pesticides discharged in the water environment can modulate the mixed-function monooxygenases (MFO) detoxification system of water organisms, the in vivo and in vitro effects of Dicuran on hepatic cytochrome P450 (cyt P450) monooxygenase activities were also examined in juvenile carp (Cyprinus carpio L.). By measuring the activities of MFO in experimental carp exposed to Dicuran an attempt was made to establish whether Dicuran could be bioactivated by MFO into ultimate mutagens. Our results on the bacterial strains Salmonella typhimurium TA100 and TA98 show that Dicuran does not possess either mutagenic or premutagenic characteristics. The micronucleus test on the erythrocytes of experimental carp did not establish any clastogenic effect either. However, Dicuran significantly inhibited the MFO activity of 7-ethoxyresorufin-O-deethylase (EROD) and benzo[a]pyrene monooxygenase (BaPMO) in the liver of experimental carp in vitro, as well as in in vivo conditions. These findings demonstrate the potentially damaging effect of Dicuran on the xenobiotic metabolizing enzyme systems of fish, and suggest the applicability of described methods for the prediction of the ecotoxicological significance of the presence of pesticides in the water environment.
Ramljak
S
S
Institut für Physiologishe Chemie, Abteilung Angewandte Molekularbiologie, Universität, Mainz, Germany.
Hackenberger
B K
BK
Smital
T
T
Britvić
S
S
D000818:Animals; D002347:Carps; D065607:Cytochrome P-450 Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D003603:Cytotoxins / Q000633:toxicity*; D006540:Herbicides / Q000633:toxicity*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D006899:Mixed Function Oxygenases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D010671:Phenylurea Compounds / Q000633:toxicity*; D012486:Salmonella typhimurium / Q000187:drug effects; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D065607:Cytochrome P-450 Enzyme Inhibitors; D003603:Cytotoxins; D006540:Herbicides; D009153:Mutagens; D010671:Phenylurea Compounds; D014874:Water Pollutants, Chemical; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; C007033:chlortoluron