MAIL, a novel nuclear I kappa B protein that potentiates LPS-induced IL-6 production.
We have identified and characterized a novel member of the ankyrin-repeat family named 'molecule possessing ankyrin-repeats induced by lipopolysaccharide' (MAIL). The C-terminal portion of MAIL shared high sequence homology with the I kappa B family. Intraperitoneal injection of lipopolysaccharide (LPS) into mice rapidly (<0.5 h) induced MAIL mRNA in various tissues, particularly in the spleen, lymph node, and lung. Ectopically expressed MAIL was localized in the nucleus, and remarkably potentiated the LPS-induced mRNA expression and secretion of interleukin (IL)-6 in Swiss 3T3 cells. These findings indicate that MAIL is one of the nuclear I kappa B proteins and an activator of IL-6 production.
Kitamura
H
H
Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan. ktmr@vetmed.hokudai.ac.jp
Kanehira
K
K
Okita
K
K
Morimatsu
M
M
Saito
M
M
D016475:3T3 Cells; D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D002467:Cell Nucleus / Q000737:chemistry; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004357:Drug Synergism; D015870:Gene Expression; D049452:Green Fluorescent Proteins; D015850:Interleukin-6 / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008164:Luminescent Proteins / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*; D009928:Organ Specificity; D012333:RNA, Messenger / Q000032:analysis; D011993:Recombinant Fusion Proteins; D017385:Sequence Homology; D014162:Transfection
D048868:Adaptor Proteins, Signal Transducing; D018076:DNA, Complementary; D015850:Interleukin-6; D008070:Lipopolysaccharides; D008164:Luminescent Proteins; C485947:Nfkbiz protein, mouse; D009687:Nuclear Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins