Distinct sprouting responses of sympathetic and peptidergic sensory axons proximal to a sciatic nerve transection in guinea pigs and rats.
Sprouting of sympathetic and peptidergic sensory neurones proximal to nerve lesions may reflect upregulation of growth factors around damaged dorsal root ganglion (DRG) cells. Axons containing noradrenaline or calcitonin gene-related peptide were visualized in DRGs and spinal roots of guinea pigs and rats. After sciatic transection in rats, varicose terminals of both types appeared around large DRG somata. These neurones were surrounded by proliferated satellite cells expressing glial fibrillary acidic protein (GFAP) and p75. This did not occur in guinea pigs. Instead, sympathetic axons grew through the DRG and centrally along the dorsal roots (which contained p75-positive glia), avoiding the DRG somata. Thus the glial reaction to peripheral injury differs between species such that, in guinea pigs, the environment in the spinal roots rather than in the DRGs favours sympathetic sprouting.
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Prince of Wales Medical Research Institute, Barker Street, Randwick, NSW 2031, Sydney, Australia.
McLachlan
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EM
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism*; D005260:Female; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism*; D006168:Guinea Pigs; D008297:Male; D009475:Neurons, Afferent / Q000166:cytology / Q000502:physiology*; D009638:Norepinephrine / Q000378:metabolism; D010525:Peripheral Nerves / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000293:injuries; D013045:Species Specificity
D005904:Glial Fibrillary Acidic Protein; D015740:Calcitonin Gene-Related Peptide; D009638:Norepinephrine