Immortalization of T lymphocytes by human T-cell leukemia virus type 1 is independent of the tax-CBP/p300 interaction.
The human T-cell leukemia virus type 1 (HTLV-1) Tax oncoprotein is a 40-kDa nuclear phosphoprotein which functions in the viral replication cycle as a transcriptional trans-activator of the viral long terminal repeat. Tax interacts with a variety of different transcription factors, including the CREB binding protein (CBP)/p300 family of transcriptional accessory proteins. We demonstrate that a Tax mutant defective for the CBP/p300 interaction retains the capacity to immortalize primary human T lymphocytes when it is expressed from a functional molecular clone of HTLV-1. Thus, immortalization of HTLV-1-infected cells appears to be independent of Tax-induced alterations in CBP/p300 function.
Robek
M D
MD
Departments of Medicine, Pathology, and Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Ratner
L
L
D050882:CREB-Binding Protein; D002460:Cell Line; D002472:Cell Transformation, Viral / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D016355:Genes, pX; D015368:Human T-lymphotropic virus 1 / Q000235:genetics; D006801:Humans; D009687:Nuclear Proteins / Q000235:genetics*; D013601:T-Lymphocytes / Q000473:pathology* / Q000821:virology*; D015534:Trans-Activators / Q000235:genetics*
D009687:Nuclear Proteins; D015534:Trans-Activators; D050882:CREB-Binding Protein; C494363:CREBBP protein, human