Double oxidation of D-xylose to D-glycero -pentos-2,3-diulose (2,3-diketo-D-xylose) by pyranose dehydrogenase from the mushroom Agaricus bisporus.
Pyranose dehydrogenase purified to homogeneity from the mycelia of the basidiomycete fungus Agaricus bisporus catalyzed the oxidation of D-xylose at C-2 to D-threo-pentos-2-ulose (2-keto-D-xylose) and successively at C-3 to D-glycero-pentos-2,3-diulose (2,3-diketo-D-xylose) using 1,4-benzoquinone as an electron acceptor. The sites of oxidation were deduced from the spectroscopic analysis (MS, NMR) of the N,N-diphenylhydrazone derivatives of the reaction products.
Volc
J
J
Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague. volc@biomed.cas.cz
Sedmera
P
P
Halada
P
P
Prikrylová
V
V
Haltrich
D
D
D000364:Agaricus / Q000201:enzymology*; D002237:Carbohydrate Dehydrogenases / Q000302:isolation & purification / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D006835:Hydrazones / Q000737:chemistry; D007659:Ketones / Q000737:chemistry / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010084:Oxidation-Reduction; D014994:Xylose / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism*
C421921:2,3-diketoxylose; D006835:Hydrazones; D007659:Ketones; D014994:Xylose; D002237:Carbohydrate Dehydrogenases