title: Comparative study of biphasic calcium phosphate ceramics impregnated with rhBMP-2 as bone substitutes.
abstract: We investigated pellet-shaped implants prepared from biphasic calcium phosphate (BCP) ceramics with five different ratios of hydroxyapatite (HAP) to beta-tricalcium phosphate (beta-TCP) to evaluate these ceramics as bone substitutes. BCP ceramics impregnated with different doses of recombinant human bone morphogenetic protein 2 (rhBMP-2) (1, 5, and 10 microg) were used for experimental purposes and ceramics without rhBMP-2 were used for control. The pellets were implanted under the pericranium in adult Wistar male rats and were harvested 8 weeks after implantation. The retrieved pellets were then examined radiologically, histologically, and histomorphometrically. The results revealed that the pellets treated with rhBMP-2 exhibited new bone and bone marrow, whereas control pellets produced fibrous connective tissues. The formation of new bone induced by rhBMP-2 was dose dependent. The extent of bone and bone marrow formation and the degree of resorption of the ceramic particles were significantly higher in the pellets composed of 25% HAP-75% TCP. In this study, bioresorption of the ceramic produced favorable conditions for rhBMP-2-induced bone formation.
authors.affiliation: Oral Surgery, Department of Oral Restitution, Division of Oral Health Science, Tokyo Medical and Dental University Graduate School, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan. izumi.osur@tmd.ac.jp
authors.forename: I
authors.initials: I
authors.lastname: Alam
authors.forename: I
authors.initials: I
authors.lastname: Asahina
authors.forename: K
authors.initials: K
authors.lastname: Ohmamiuda
authors.forename: S
authors.initials: S
authors.lastname: Enomoto
mesh_terms: D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials; D055395:Bone Morphogenetic Protein 1; D019485:Bone Morphogenetic Proteins / Q000737:chemistry*; D018786:Bone Substitutes / Q000737:chemistry*; D001842:Bone and Bones / Q000166:cytology / Q000000981:diagnostic imaging; D002130:Calcium Phosphates / Q000737:chemistry*; D002516:Ceramics / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry; D008297:Male; D008422:Materials Testing; D008666:Metalloendopeptidases / Q000737:chemistry*; D011859:Radiography; D051381:Rats; D017208:Rats, Wistar; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D019485:Bone Morphogenetic Proteins; D018786:Bone Substitutes; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; D017886:Durapatite; D008666:Metalloendopeptidases; C525705:BMP1 protein, human; C525708:Bmp1 protein, rat; D055395:Bone Morphogenetic Protein 1; C018392:tricalcium phosphate