Solid-phase extraction and liquid chromatographic quantitation of quinfamide in biological samples.
This paper describes a high-performance liquid chromatographic method for the assay of quinfamide and its main metabolite, 1-(dichloroacetyl)-1,2,3,4,-tetrahydro-6-quinolinol, in plasma, urine and feces. It requires 1 ml of biological fluid, an extraction using Sep-Pack cartridges and acetonitrile for drug elution. Analysis was performed on a CN column (5 microm) using water-acetonitrile-methanol (40:50:10) as a mobile phase at 269 nm. Results showed that the assay was linear in the range between 0.08 and 2.0 microg/ml. The limit of quantitation was 0.08 microg/ml. Maximum assay coefficient of variation was 14%. Recovery obtained in plasma, urine and feces ranged from 82% to 98%.
Morales
J M
JM
Departmento de Farmacia, Conjunto E de la Facultad de Química, UNAM, Ciudad Universitaria, Mexico, DF Mexico.
Jung
C H
CH
Alarcón
A
A
Barreda
A
A
D000563:Amebicides / Q000032:analysis* / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005243:Feces / Q000737:chemistry*; D006801:Humans; D011804:Quinolines / Q000032:analysis* / Q000097:blood / Q000652:urine; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000563:Amebicides; D011804:Quinolines; C026546:quinfamide