Cytometric methods to analyze ionizing-radiation effects.
Four cytometric assays for the assessment of radiation-induced DNA damage in individual cells are presented. Two of these, the alkaline and neutral comet assays, are useful for the detection of DNA damage due to very low radiation doses and promise to be useful for the quantitation of genomic damage after clinically or environmentally relevant exposures. The other two, the halo and halo-comet assays, reveal aspects of chromatin structure in the presence of DNA damage that reflect differences in intrinsic cellular radiosensitivity. Further development of these assays used alone, or in combination, should eventually lead to the definition of readily measurable cytometric parameters that will be useful as predictive markers for cellular responses to DNA damaging agents.
Wright
W D
WD
Mallinckrodt Institute of Radiology, Washington University School of Medicine, St. Louis, Missouri 63108, USA.
Lagroye
I
I
Zhang
P
P
Malyapa
R S
RS
Roti
J L
JL
D000818:Animals; D016466:CHO Cells; D020552:Comet Assay / Q000295:instrumentation / Q000379:methods*; D006224:Cricetinae; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000528:radiation effects*; D004249:DNA Damage; D004307:Dose-Response Relationship, Radiation; D005434:Flow Cytometry / Q000379:methods*; D008856:Microscopy, Fluorescence; D009690:Nucleic Acid Conformation; D011839:Radiation, Ionizing; D012815:Signal Processing, Computer-Assisted
D004247:DNA