Use of molecular beacons to detect an antifolate resistance-associated mutation in Plasmodium falciparum.
A PCR-based technique using new fluorescent probes, called molecular beacons, was developed to detect the antifolate resistance-associated S108N point mutation in Plasmodium falciparum. One hundred African clinical isolates were tested by the new method in comparison with the PCR-restriction fragment length polymorphism method. This new molecular technique appears to be a promising tool for epidemiological studies.
Durand
R
R
Centre National de Référence pour la Chimiosensibilité du Paludisme, Université Paris V et Assistance Publique-Hôpitaux de Paris, Hôpital Bichat-Claude Bernard, Laboratoire de Parasitologie, Paris, France. rjdurand@club-internet.fr
Eslahpazire
J
J
Jafari
S
S
Delabre
J F
JF
Marmorat-Khuong
A
A
di Piazza
J P
JP
Le Bras
J
J
D000818:Animals; D004351:Drug Resistance / Q000235:genetics; D005493:Folic Acid Antagonists / Q000494:pharmacology*; D006801:Humans; D009154:Mutation; D010963:Plasmodium falciparum / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*
D005493:Folic Acid Antagonists; D013762:Tetrahydrofolate Dehydrogenase