How motor proteins influence microtubule polymerization dynamics.
The interplay between microtubules and microtubule-based motors is fundamental to basic aspects of cellular function, such as the intracellular transport of organelles and alterations in cellular morphology during cell locomotion and division. Motor proteins are unique in that they couple nucleotide hydrolysis to force production that can do work. The force transduction by proteins belonging to the kinesin and dynein superfamilies has been thought only to power movement of these motors along the surface of microtubules; however, a growing body of evidence, both genetic and biochemical, suggests that motors can also directly influence the polymerization dynamics of microtubules. For example, at the vertebrate kinetochore, motors interact directly with microtubule ends and modulate polymerization dynamics to orchestrate chromosome movements during mitosis. Although a role for motors in regulating microtubule length has been established, the mechanisms used by motors to promote microtubule growth or shrinkage are unclear, as is an understanding of why cells might choose motors to control dynamics rather than a variety of non-motor proteins known to affect microtubule stability. Elucidation of the exact mechanisms by which motors alter the exchange of tubulin subunits at microtubule ends in vitro may shed light on how microtubule stability is regulated to produce the array of dynamic behavior seen in cells.
Hunter
A W
AW
Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle, WA 98195 USA.
Wordeman
L
L
D000595:Amino Acid Sequence; D000818:Animals; D016547:Kinesins / Q000378:metabolism; D018386:Kinetochores / Q000378:metabolism / Q000502:physiology; D008868:Microtubule Proteins; D008869:Microtubule-Associated Proteins / Q000378:metabolism / Q000502:physiology*; D008870:Microtubules / Q000378:metabolism / Q000502:physiology*; D020409:Molecular Motor Proteins; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D050777:Stathmin
D008868:Microtubule Proteins; D008869:Microtubule-Associated Proteins; D020409:Molecular Motor Proteins; D010750:Phosphoproteins; D050777:Stathmin; D016547:Kinesins