A beta-D-glucan from the sclerotia of Pleurotus tuber-regium (Fr.) Sing.
An alkali-soluble polysaccharide (Hunai polysaccharide, 1) from the fruit body of Pleurotus tuber-regium (Fr.) Sing was shown to be homogeneous by gel permeation chromatography and its molecular weight was approximately 4.3 x 10(5). Complete acid hydrolysis, periodate oxidation, Smith degradation, methylation, FT-IR and 13C NMR analysis, complex formation with Congo Red, indicated that 1 has a beta-(1 --> 3)-linked D-glucopyranosyl backbone with a single beta-D-glucopyranosyl group at O-6 of every third glucose residue.
Chenghua
D
D
Department of Chemistry, Huazhong University of Science and Technology, Wuhan , PR China.
Xiangliang
Y
Y
Xiaoman
G
G
Yan
W
W
Jingyan
Z
Z
Huibi
X
X
D002236:Carbohydrate Conformation; D002850:Chromatography, Gel; D005936:Glucans / Q000737:chemistry* / Q000302:isolation & purification; D006868:Hydrolysis; D008970:Molecular Weight; D019906:Nuclear Magnetic Resonance, Biomolecular; D018514:Plant Structures / Q000737:chemistry; D020076:Pleurotus / Q000737:chemistry*
D005936:Glucans