Larger intercellular variation in (Q/R) editing of GluR6 than GluR5 revealed by single cell RT-PCR.
RNA editing of the pre-mRNA encoding the kainate receptor subtypes determines the Ca2+ permeability and the rectifying properties of the receptors in which these are assembled. GluR6 pre-mRNA contains three characterized editing sites: Q/R, IN and the Y/C, whereas GluR5 pre-mRNA contains only the (Q/R) site. Single cell RT-PCR was used on cultured cortical neurons to determine the relative expression and editing levels of the kainate receptor subunits encoding mRNA. The analysis showed a large intercellular variation in editing efficiency. The overall lower level of GluR5 editing, in the culture, compared to GluR6 editing is a result of an approximately 60% lower editing efficiency of GluR5 pre-mRNA, within single cells, compared with GluR6.
Christensen
K V
KV
Department of Molecular and Structural Biology C.F. Mollers Allé, University of Aarhus, Denmark.
Dai
W M
WM
Lambert
J D
JD
Egebjerg
J
J
D000818:Animals; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000502:physiology*; D004622:Embryo, Mammalian; D014644:Genetic Variation; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D017393:RNA Editing; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018092:Kainic Acid Receptors / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000097806:GluK2 Kainate Receptor; D000097808:GluK3 Kainate Receptor
D012322:RNA Precursors; D018092:Kainic Acid Receptors; D000097806:GluK2 Kainate Receptor; D000097808:GluK3 Kainate Receptor