Calcium-independent phospholipase A(2): structure and function.
The classical Ca(2+)-independent phospholipase A(2) enzyme, now known as Group VIA PLA(2), was initially purified and characterized from the P388D(1) macrophage-like cell line. The corresponding cDNA was subsequently cloned from a variety of sources, and it is now known that multiple splice variants of the enzyme are expressed, some of which may act as negative regulators of the active enzyme. Group VIA PLA(2) has a consensus lipase motif (GTSTG) containing the catalytic serine, is 85-88 kDa, and exists in an aggregated form. The enzyme contains multiple ankyrin repeats, which may play a role in oligomerization. The Group VIA enzyme exhibits lysophospholipase activity as well as phospholipase A(2) activity, and it is capable of hydrolyzing a wide variety of phospholipid substrates. A major function of Group VIA PLA(2) is to mediate phospholipid remodeling, but the enzyme may play other roles as well. Other Ca(2+)-independent PLA(2) enzymes have more recently been identified, and it may be possible to discriminate between the various Ca(2+)-independent PLA(2) enzymes based on sequence or inhibitor-sensitivity. However, the physiological functions of the newly identified enzymes have yet to be elucidated.
Winstead
M V
MV
Department of Chemistry and Biochemistry, 0601, Revelle College and School of Medicine, University of California at San Diego, 92093-0601, USA.
Balsinde
J
J
Dennis
E A
EA
D000255:Adenosine Triphosphate / Q000737:chemistry; D000595:Amino Acid Sequence; D000818:Animals; D017487:Ankyrins / Q000737:chemistry; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D016384:Consensus Sequence; D005786:Gene Expression Regulation; D006801:Humans; D007527:Isoenzymes / Q000737:chemistry; D010741:Phospholipases A / Q000737:chemistry* / Q000145:classification / Q000378:metabolism; D012694:Serine / Q000737:chemistry; D015398:Signal Transduction; D013347:Subcellular Fractions / Q000201:enzymology
D017487:Ankyrins; D007527:Isoenzymes; D012694:Serine; D000255:Adenosine Triphosphate; D010741:Phospholipases A; D002118:Calcium