High-frequency flp recombinase-mediated inversions of the oriC-containing region of the Pseudomonas aeruginosa genome.
The genomes of the two clonally derived Pseudomonas aeruginosa prototypic strains PAO1 and DSM-1707 differ by the presence of a 2. 19-Mb inversion including oriC. Integration of two Flp recombinase target sites near the rrn operons containing the inversion endpoints in PAO1 led to Flp-catalyzed inversion of the intervening 1.59-Mb fragment, including oriC, at high frequencies (83%), favoring the chromosome configuration found in DSM-1707. The results indicate that the oriC-containing region of the P. aeruginosa chromosome can readily undergo and tolerate large inversions.
Barekzi
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N
Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523, USA.
Beinlich
K
K
Hoang
T T
TT
Pham
X Q
XQ
Karkhoff-Schweizer
R
R
Schweizer
H P
HP
D001483:Base Sequence; D007446:Chromosome Inversion; D002874:Chromosome Mapping; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000235:genetics*; D018741:Replication Origin / Q000235:genetics*; D017422:Sequence Analysis, DNA
D004254:DNA Nucleotidyltransferases; C045119:FLP recombinase