title: Distinct regulation of brain mitochondrial carrier protein-1 and uncoupling protein-2 genes in the rat brain during cold exposure and aging.
abstract: Mitochondrial uncoupling proteins (UCPs) have been postulated to be regulators of thermogenesis, energy balance, and oxidative stress. Brain mitochondrial carrier protein-1 (BMCP1) is a new member of the UCP family, but little is known about the gene regulation and the role of BMCP1 in the central nervous system. In the present study, we first cloned BMCP1 cDNA encoding 325 amino acids from rat brain. The BMCP1 mRNA showed a distinct distribution pattern compared with that of UCP2 gene in human brain. Cold exposure did not affect the mRNA levels of BMCP1 and UCP2 in rat whole brain, but did increase the expression of UCP2 in the spinal cord. The mRNA level of BMCP1 in the brain of 26-month-old rats was decreased by 30% and that of UCP2 increased by 60% compared with the levels in 6-month-old rats. These results suggest differential roles of BMCP1 and UCP2 in thermoregulation and aging.
authors.affiliation: Department of Molecular Genetics, National Institute for Longevity Sciences, 36-3 Gengo, Obu, 474-8522, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Mizuno
authors.forename: T
authors.initials: T
authors.lastname: Miura-Suzuki
authors.forename: H
authors.initials: H
authors.lastname: Yamashita
authors.forename: N
authors.initials: N
authors.lastname: Mori
mesh_terms: D000064:Acclimatization; D000375:Aging / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000254:growth & development / Q000378:metabolism*; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D003080:Cold Temperature; D005786:Gene Expression Regulation; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D007473:Ion Channels; D008297:Male; D026901:Membrane Transport Proteins; D051379:Mice; D008928:Mitochondria / Q000378:metabolism; D033681:Mitochondrial Membrane Transport Proteins; D024101:Mitochondrial Proteins; D000071245:Mitochondrial Uncoupling Proteins; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics*; D011506:Proteins / Q000235:genetics*; D051381:Rats; D011916:Rats, Inbred F344; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D000071246:Uncoupling Protein 2
chemical_list: D002352:Carrier Proteins; D007473:Ion Channels; D026901:Membrane Transport Proteins; D033681:Mitochondrial Membrane Transport Proteins; D024101:Mitochondrial Proteins; D000071245:Mitochondrial Uncoupling Proteins; D009419:Nerve Tissue Proteins; D011506:Proteins; C116584:SLC25A14 protein, human; C492124:Slc25a14 protein, mouse; C492125:Slc25a14 protein, rat; C000604389:UCP2 protein, human; C000604390:Ucp2 protein, mouse; C000604391:Ucp2 protein, rat; D000071246:Uncoupling Protein 2