SIVcpz from a naturally infected Cameroonian chimpanzee: biological and genetic comparison with HIV-1 N.
Thus far, simian immunodeficiency virus from chimpanzees (SIVcpz) genomes have been characterized as Pan troglodytes troglodytes and show a strong relation with human immunodeficiency virus (HIV)-1 N in their env genes. We fully characterized another SIVcpz from P. t. troglodytes. This chimpanzee (Cam5) was, as was also the host of SIVcpz-cam3, wild born in Cameroon, a region where all three groups of HIV-1 (M, N and O) co-occur. In contrast to other SIVcpz, SIVcpz-cam5 was isolated immediately after the rescue of the animal. Our data demonstrate that SIVcpz-cam5, like SIVcpz-cam3, grows easily on human peripheral blood mononuclear cells (PBMCs) and uses CCR5 as a co-receptor similar to HIV-1 N YBF30. Phylogenetic analysis based on the entire env gene shows that SIVcpz-cam5 falls into the same unique subcluster as HIV-1 N YBF30, SIVcpz-cam3 and SIVcpz-US. A phylogenetic relationship was also found with the vif gene of HIV-1 N. This study provides proof that HIV-1 N related viruses circulate in wild P. t. troglodytes.
Müller-Trutwin
M C
MC
Unité de Biologie des Rétrovirus, Institut Pasteur, Paris, France.
Corbet
S
S
Souquière
S
S
Roques
P
P
Versmisse
P
P
Ayouba
A
A
Delarue
S
S
Nerrienet
E
E
Lewis
J
J
Martin
P
P
Simon
F
F
Barré-Sinoussi
F
F
Mauclère
P
P
D000595:Amino Acid Sequence; D000818:Animals; D002163:Cameroon; D016384:Consensus Sequence; D004279:DNA, Viral / Q000235:genetics; D015686:Gene Products, env / Q000737:chemistry / Q000235:genetics; D015752:Genes, env; D015497:HIV-1 / Q000145:classification / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008214:Lymphocytes / Q000276:immunology / Q000821:virology; D008969:Molecular Sequence Data; D002679:Pan troglodytes; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D015728:Receptors, HIV / Q000502:physiology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D016097:Simian Acquired Immunodeficiency Syndrome / Q000097:blood / Q000276:immunology / Q000821:virology*; D015302:Simian Immunodeficiency Virus / Q000145:classification / Q000235:genetics / Q000502:physiology*
D004279:DNA, Viral; D015686:Gene Products, env; D015728:Receptors, HIV