title: Three states of the pFh-fragment (Hinge region) from human myeloma IgG3 Kuc with native-like secondary structure.
abstract: The structure of the pFh-fragment (hinge region) from human myeloma IgG3 Kuc (the third subclass immunoglobulin) was studied by hydrodynamic methods in the pH range from 3.0 to 8.0. The pFh-fragment was found to occur in three states, each with a high content of the secondary structure: a rod-like state at pH < or = 4.0, a "molten globule" state at pH 4.2-5.5, and the native state at pH 7. 5-8.0.
authors.affiliation: Institute of Biological Instrumentation, Russian Academy of Sciences, Pushchino, Moscow Region, 142292, Russia.
authors.forename: V M
authors.initials: VM
authors.lastname: Tischenko
mesh_terms: D000595:Amino Acid Sequence; D002942:Circular Dichroism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007128:Immunoglobulin Fragments / Q000737:chemistry; D007074:Immunoglobulin G / Q000737:chemistry*; D066298:In Vitro Techniques; D009194:Myeloma Proteins / Q000737:chemistry*; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D013816:Thermodynamics
chemical_list: D007128:Immunoglobulin Fragments; D007074:Immunoglobulin G; D009194:Myeloma Proteins