title: BCL-2 regulation targeting the AU-rich domain.
abstract: In an effort to identify potential upregulators of bcl-2 activity in t(14;18) follicular B lymphoma cells, we detected a hybrid bcl-2/IgH RNA transcribed in antisense orientation. This antisense transcript may contribute to upregulation of bcl-2 expression in t(14;18) cells, overlapping AU-rich motifs present in the 3'-untranslated region of bcl-2 mRNA. We have studied the enzymatic efficiency of a ribozyme directed towards the bcl-2 AU-rich region in a cell-free system determining its kinetic parameters.
authors.affiliation: Department of Pharmacology, University of Milan, Italy. annamaria.bevilacqua@unimi.it
authors.forename: A
authors.initials: A
authors.lastname: Bevilacqua
authors.forename: M C
authors.initials: MC
authors.lastname: Ceriani
authors.forename: N
authors.initials: N
authors.lastname: Schiavone
authors.forename: A
authors.initials: A
authors.lastname: Nicolin
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics*; D020029:Base Pairing; D002051:Burkitt Lymphoma / Q000473:pathology; D002474:Cell-Free System; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics / Q000648:ultrastructure; D002887:Chromosomes, Human, Pair 18 / Q000235:genetics / Q000648:ultrastructure; D015972:Gene Expression Regulation, Neoplastic; D005803:Genes, Immunoglobulin; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007700:Kinetics; D008224:Lymphoma, Follicular / Q000235:genetics / Q000473:pathology; D017434:Protein Structure, Tertiary; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000737:chemistry / Q000502:physiology*; D016372:RNA, Antisense / Q000235:genetics*; D016337:RNA, Catalytic / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D013379:Substrate Specificity; D014178:Translocation, Genetic / Q000235:genetics; D014407:Tumor Cells, Cultured
chemical_list: D020413:3' Untranslated Regions; D007143:Immunoglobulin Heavy Chains; D019253:Proto-Oncogene Proteins c-bcl-2; D016372:RNA, Antisense; D016337:RNA, Catalytic; D012333:RNA, Messenger; D012334:RNA, Neoplasm; C421446:hammerhead ribozyme RZ3-BL2