[Contribution to the differentiation of cross-reacting antibodies in brucellosis serology--1. Reactions with various Yersinia serotypes and antibody avidity].
Brucella and Yersinia enterocolitca O:9 (Y.e.O:9) have antigenic determinants in common causing cross-reactions which interfere strongly in Brucella serology. Hoffmann (1988) observed cross-reactivity between Y.e.O:9, Y.e.O:5, and Y.e.O:6 and used an ELISA for the differentiation between Brucella- and Yersinia antibodies since Y.e.O:5 and Y.e.O:6 produced only low-level cross-reactions with Brucella antigen. The observations of Hoffmann were confirmed and led to the construction of an ELISA with 4 antigens. Sera from natural outbreaks of brucellosis and from artificial infections with Brucella and Y.e.O:9 were properly identifiable by this system. Furthermore, the test material has been used to measure the antibody avidity by a specially designed ELISA. This test produced a further divergence between specific and non-specific reactions.
Staak
C
C
Bundesinstitut für gesundheitlichen Verbraucherschutz und Veterinärmedizin, Berlin.
Draeger
A
A
Bahn
P
P
Nöckler
K
K
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000276:immunology*; D002002:Brucella / Q000145:classification* / Q000276:immunology; D002006:Brucellosis / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D003429:Cross Reactions; D003937:Diagnosis, Differential; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes; D012703:Serotyping; D015007:Yersinia / Q000145:classification* / Q000276:immunology; D015009:Yersinia Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D015008:Yersinia enterocolitica
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D000939:Epitopes