Molecular characterization of the Sinorhizobium meliloti nlpD gene.
The Sinorhizobium meliloti nlpD gene consists of 1,539 nucleotides and codes for 512 amino acids. Expression of the nlpD gene as a histidine-tagged protein in Escherichia coli resulted in the production of a 57-kDa protein. The deduced polypeptide sequence of NlpD contains one unusual hexamer repeat (KVQRGQ), one tetramer (TVTV) and two direct and inverted trimer repeats (KAA, AAK). The N-terminal amino acid residues displayed similarity with signal peptides of secreted bacterial lipoproteins. Mutations of the S. meliloti nlpD gene caused decreased survival of cells in the stationary phase.
Streit
W R
WR
Institut für Mikrobiologie und Genetik der Universität Göttingen, Germany. wstreit@gwdg.de
Hofmann
K
K
Liebl
W
W
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D003470:Culture Media; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D006639:Histidine / Q000378:metabolism; D008074:Lipoproteins / Q000235:genetics* / Q000378:metabolism*; D000455:Medicago sativa / Q000382:microbiology; D008969:Molecular Sequence Data; D018517:Plant Roots / Q000382:microbiology; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D016962:Sinorhizobium meliloti / Q000235:genetics* / Q000254:growth & development
D001426:Bacterial Proteins; C405030:BioS protein, Rhizobium meliloti; D003470:Culture Media; D029968:Escherichia coli Proteins; D008074:Lipoproteins; C490122:NlpD protein, E coli; C086389:NlpD protein, bacteria; D011993:Recombinant Fusion Proteins; D006639:Histidine