D-mannoheptulose uptake and its metabolic and secretory effects in human pancreatic islets.
D-mannoheptulose was recently proposed to be transported into cells at the intervention of GLUT2. Since GLUT1, rather than GLUT2, represents the major carrier system for the transport of monosaccharides across the islet B-cell plasma membrane in human subjects, the uptake of D-mannoheptulose and its metabolic and secretory effects were investigated in human islets. The uptake of D-glucose reached much more rapidly a close-to-equilibrium value in isolated islets than in pieces of pancreas obtained from the same donor. The distribution space of D-[3H]mannoheptulose in the human islets largely exceeded that of [U-14C]sucrose, considered as an extracellular marker, and did not differ significantly from that of 3HOH. In the human islets, the heptose (10.0 mM) inhibited both D-[5-3H]glucose utilization and D-[U-14C] glucose oxidation, and decreased glucose-stimulated insulin release to the same extent as D-mannoheptulose hexaacetate. These findings indicate that a suitable radioactive analog of D-mannoheptulose could be used, in human like in rat islets, for preferential labelling of the endocrine moiety of the pancreatic gland.
Sener
A
A
Laboratory of Experimental Medicine, Brussels Free University, Brussels, Belgium.
Ladriere
L
L
Benito
C
C
Gomis
R
R
Malaisse-Lagae
F
F
Malaisse
W J
WJ
D002250:Carbon Radioisotopes; D005947:Glucose / Q000378:metabolism / Q000493:pharmacokinetics; D006801:Humans; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism*; D008356:Mannoheptulose / Q000378:metabolism* / Q000493:pharmacokinetics
D002250:Carbon Radioisotopes; D007328:Insulin; D008356:Mannoheptulose; D005947:Glucose