A primitive myoglobin from Tetrahymena pyriformis: its heme environment, autoxidizability, and genomic DNA structure.
A myoglobin-like protein isolated from Tetrahymena pyriformis is composed of 121 amino acid residues. This is much smaller than sperm whale myoglobin by 32 residues, suggesting a distinct origin from the common globin gene. We have therefore examined this unique protein for its structural, spectral and stability properties. As a result, the rate of autoxidation of Tetrahymena oxymyoglobin (MbO(2)) was found to be almost comparable to that of sperm whale MbO(2) over a wide range of pH 4-12 in 0.1 M buffer at 25 degrees C. Moreover, both pH profiles exhibited the remarkable proton-assisted process, which can be performed in sperm whale myoglobin by the distal (E7) histidine as its catalytic residue. These kinetic observations are also in full accord with spectral examinations for the presence of a distal histidine in ciliated protozoa myoglobin. At the same time, we have isolated the globin genes both from T. pyriformis and Tetrahymena thermophila, and found that there is no intron in their genomic structures. This is in sharp contrast to previous reports on the homologous globin genes from Paramecium caudatum and Chlamydomonas eugametos. Rather, the Tetrahymena genes seemed to be related to the cyanobacterial globin gene from Nostoc commune. These contracted or truncated globins thus have a marked diversity in the cDNA, protein, and genomic structures.
Korenaga
S
S
Biological Institute, Graduate School of Science, Tohoku Unviersity, Sendai 980-8578, Japan.
Igarashi
J
J
Matsuoka
A
A
Shikama
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004578:Electron Spin Resonance Spectroscopy; D019143:Evolution, Molecular; D018503:Genome, Protozoan; D006639:Histidine / Q000032:analysis; D007700:Kinetics; D008969:Molecular Sequence Data; D009211:Myoglobin / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D010084:Oxidation-Reduction; D011487:Protein Conformation; D015800:Protozoan Proteins / Q000302:isolation & purification*; D016415:Sequence Alignment; D013053:Spectrophotometry; D013769:Tetrahymena pyriformis / Q000378:metabolism*
D009211:Myoglobin; D015800:Protozoan Proteins; C017595:oxymyoglobin; D006639:Histidine