Bombesin-like peptide and receptors in lung injury models: diverse gene expression, similar function.
We previously demonstrated that bombesin-like peptide (BLP) mediates lung injury in premature infants with bronchopulmonary dysplasia (BPD). We now investigate gene expression and function of BLP (gastrin-releasing peptide, GRP) and BLP-receptors (GRP-R and BRS-3) in lung from two baboon BPD models. In the "interrupted gestation model," only GRP mRNA was up-regulated. In the "hyperoxic model," GRP-R mRNA was up-regulated. In lung explants from O2-treated animals, all BPD animals responded to 1nM bombesin, whereas non-BPD animals did not; the opposite effect was observed with a BLP blocking antibody. Cumulatively, these observations suggest that novel BLPs and/or BLP receptors are likely to be implicated in the pathogenesis of BPD.
Cullen
A
A
Department of Medicine, Division of Neonatology, Children's Hospital and Harvard Medical School, Boston, MA, USA.
Emanuel
R L
RL
Torday
J S
JS
Asokananthan
N
N
Sikorski
K A
KA
Sunday
M E
ME
D000818:Animals; D001839:Bombesin / Q000378:metabolism / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D019886:Gastrin-Releasing Peptide / Q000096:biosynthesis* / Q000502:physiology*; D015870:Gene Expression; D017403:In Situ Hybridization; D008168:Lung / Q000196:embryology / Q000378:metabolism*; D009924:Organ Culture Techniques; D010100:Oxygen / Q000378:metabolism; D010215:Papio / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D018004:Receptors, Bombesin / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D015854:Up-Regulation
D010713:Phosphatidylcholines; D012333:RNA, Messenger; D018004:Receptors, Bombesin; C107120:bombesin receptor subtype 3; C070167:di-(8-methylstearoyl)phosphatidylcholine; D003907:Dexamethasone; D019886:Gastrin-Releasing Peptide; D001839:Bombesin; D010100:Oxygen