title: Experimental model of anorectal transplantation.
abstract: BACKGROUND
  Anorectal transplantation with pudendal nerve anastomosis after rectal excision is a possible strategy that would avoid a colostomy and recreate potentially normal anorectal function. This study investigates the technical feasibility of anorectal transplantation with pudendal nerve and inferior mesenteric artery and vein anastomosis in a porcine model.
  
  
  METHODS
  Four female pigs (22-42 kg) provided donor anorectum for four male recipients (29-39 kg) under standard general anaesthesia. The donor operation involved abdominoperineal excision of rectum (APR) taking the anal sphincter, pudendal neurovascular bundle and inferior mesenteric vessels. The recipient underwent APR, transperineal introduction of the donor graft, anastomoses of the rectum, inferior mesenteric vessels and pudendal neurovascular bundle, and perineal closure. Recorded variables were duration of each step of transplantation, ischaemic time, dimensions of anastomosed structures and postoperative graft viability. Animals were killed at 24 h, the state of the graft was noted and tissue was taken for confirmatory histology.
  
  
  RESULTS
  Mean operation time was 372 (range 303-435)min. Mean ischaemic time was 118 (100-130)min. Before death, observation at laparotomy revealed two pink grafts, one slightly dusky but healthy graft and one outright failure, reflecting the state of the mesenteric vessels, which were patent in three and thrombosed in one. Histological examination showed no difference between control biopsies and the three cases with satisfactory mesenteric flow. Gross ischaemia was present histologically in the failed case.
  
  
  CONCLUSIONS
  Anorectal transplantation is technically feasible in a pig model. Longer-term studies are now needed to assess return of function and overcome rejection issues.
authors.affiliation: St Marks Hospital and Northwick Park Institute for Medical Research, Harrow, UK.
authors.forename: A
authors.initials: A
authors.lastname: O'Bichere
authors.forename: S
authors.initials: S
authors.lastname: Shurey
authors.forename: P
authors.initials: P
authors.lastname: Sibbons
authors.forename: C
authors.initials: C
authors.lastname: Green
authors.forename: R K
authors.initials: RK
authors.lastname: Phillips
mesh_terms: D001003:Anal Canal / Q000294:innervation / Q000637:transplantation; D000818:Animals; D001166:Arteriovenous Shunt, Surgical / Q000379:methods; D005260:Female; D006085:Graft Survival; D008297:Male; D012002:Rectal Diseases / Q000601:surgery*; D012007:Rectum / Q000294:innervation / Q000637:transplantation*; D013552:Swine