Determination of roxithromycin residues in the flounder muscle with electrospray liquid chromatography-mass spectrometry.
A highly sensitive and specific method for the determination of roxithromycin in the flounder muscle by LC-MS was developed and validated. A dichloromethane extract of the sample was separated on C18 reversed-phase column with acetonitrile-50 mM ammonium acetate (80:20, v/v) as the mobile phase and analyzed by LC-MS via atmospheric pressure ionization/electrospray ionization interface. The limit of detection and limit of quantitation were 0.01 and 0.1 ng/g, respectively. Mean recoveries from spiked muscles were 81.1% (ranged from 71.0 to 90.3%) for roxithromycin. The method has been successfully applied to determine roxithromycin in the flounder muscle.
Lim
J
J
Division of Veterinary Pharmacology and Toxicology, Chungnam National University, Taejon, South Korea.
Jang
B
B
Lee
R
R
Park
S
S
Yun
H
H
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D005432:Flounder; D013058:Mass Spectrometry; D009132:Muscles / Q000737:chemistry*; D015203:Reproducibility of Results; D015575:Roxithromycin / Q000032:analysis*; D012680:Sensitivity and Specificity
D000900:Anti-Bacterial Agents; D015575:Roxithromycin