Localization and association to cytoskeleton of COLL1alpha mRNA in Paracentrotus lividus egg requires cis- and trans-acting factors.
COLL1alpha mRNA is asymmetrically distributed in the Paracentrotus lividus egg. Here we examine the involvement of the cytoskeleton in the localization process of collagen mRNA. The use of drugs such as colchicine and cytochalasin B reveals a perturbation of localization collagen mRNA. Moreover, the presence of specific cis-and trans-acting factors involved in cytoskeleton binding and the localization process was investigated. By Northwestern experiment we found that the 3'UTR of COLL1alpha mRNA is also able to bind two proteins of 54 and 40 kDa in a cellular fraction containing the cytoskeleton. Finally, we found that the protein of 54 kDa is LP54, a protein that binds the 3'UTRs of P. lividus maternal bep messengers and is necessary for their localization.
Romancino
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DP
Istituto di Biologia dello Sviluppo CNR, via Ugo La Malfa 153, 90146, Palermo, Italy.
Dalmazio
S
S
Cervello
M
M
Montana
G
G
Virruso
L
L
Bonura
A
A
Gambino
R
R
Di Carlo
M
M
D020413:3' Untranslated Regions; D000818:Animals; D015152:Blotting, Northern; D002458:Cell Fractionation; D003078:Colchicine / Q000494:pharmacology; D003094:Collagen / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism*; D003571:Cytochalasin B / Q000494:pharmacology; D003599:Cytoskeleton / Q000378:metabolism*; D018076:DNA, Complementary / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008870:Microtubules / Q000378:metabolism; D010063:Ovum / Q000187:drug effects / Q000378:metabolism*; D011485:Protein Binding; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012617:Sea Urchins / Q000196:embryology*; D015533:Transcriptional Activation
D020413:3' Untranslated Regions; D018076:DNA, Complementary; C401681:LP54 protein, Paracentrotus lividus; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D003571:Cytochalasin B; D003094:Collagen; D003078:Colchicine