title: Heart cell transplantation improves heart function in dilated cardiomyopathic hamsters.
abstract: BACKGROUND
  Little is known about the effect of heart cell transplantation into the dilated cardiomyopathic myocardium. This study was designed to evaluate the effect of heart cell transplantation into dilated cardiomyopathic hamsters.
  
  
  RESULTS
  Ventricular heart cells were isolated from 4-week-old BIO 53. 58 hamsters and cultured for 2 weeks before transplantation. The cells were labeled with bromodeoxyuridine (BrdU) before transplantation for identification. Adult hamsters (17 weeks old) were used as recipients. Heart cells (4 x 10(6) cells) or culture medium was transplanted into the left ventricular free wall (transplantation and control groups, respectively, n=12 each). Sham-operated hamsters (n=12) underwent the surgery but not the transplantation. Cyclosporine A was administered subcutaneously to all hamsters daily after the operation. Four weeks after the transplantation, heart function was evaluated with the use of a Langendorff preparation. Histology showed severe focal myocardial necrosis in all groups. BrdU-stained tissue was found at the cell transplantation sites. The transplanted hearts had greater (P:<0. 001) developed pressures at all balloon volumes and improved dP/dt (transplantation 915+/-253 versus control 453+/-120 and sham 530+/-187 mm Hg/s, P:<0.001, balloon volume of 15 microL). No differences in ventricular function were found between control and sham-operated hamsters.
  
  
  CONCLUSIONS
  The transplanted ventricular heart cells formed cardiac-like tissue in cardiomyopathic myocardium and improved its contractile function.
authors.affiliation: Division of Cardiovascular Surgery, Department of Surgery, Toronto General Hospital, University of Toronto, Toronto, Ontario, Canada.
authors.forename: K J
authors.initials: KJ
authors.lastname: Yoo
authors.forename: R K
authors.initials: RK
authors.lastname: Li
authors.forename: R D
authors.initials: RD
authors.lastname: Weisel
authors.forename: D A
authors.initials: DA
authors.lastname: Mickle
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Jia
authors.forename: E J
authors.initials: EJ
authors.lastname: Kim
authors.forename: S
authors.initials: S
authors.lastname: Tomita
authors.forename: T M
authors.initials: TM
authors.lastname: Yau
mesh_terms: D000818:Animals; D001794:Blood Pressure; D001973:Bromodeoxyuridine; D002306:Cardiac Volume; D002311:Cardiomyopathy, Dilated / Q000473:pathology / Q000601:surgery*; D002478:Cells, Cultured; D006224:Cricetinae; D003971:Diastole; D004195:Disease Models, Animal; D006085:Graft Survival; D006352:Heart Ventricles / Q000166:cytology* / Q000637:transplantation*; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008297:Male; D009206:Myocardium / Q000166:cytology* / Q000378:metabolism / Q000473:pathology; D018995:Myosin Heavy Chains / Q000378:metabolism; D018994:Myosin Light Chains / Q000378:metabolism; D014336:Troponin / Q000378:metabolism; D016277:Ventricular Function, Left / Q000502:physiology
chemical_list: D018994:Myosin Light Chains; D014336:Troponin; D018995:Myosin Heavy Chains; D001973:Bromodeoxyuridine