Environmental acclimatization of the carp modulates the transcription of beta-actin.
A cascade of mechanisms involving changes in gene expression are substantial to shape the adaptive responses that a eurythermal fish requires upon environmental changes in its habitat. We have previously shown that the cyclical reprogramming of rRNA transcription is a remarkable feature in carp under seasonal acclimatization. Using in situ hybridization and competitive RT-PCR we found significant differences in beta-actin transcripts, generally accepted to be coded by a typical housekeeping gene, in tissues from summer- and winter-acclimatized carp. The physiological differential beta-actin transcription herein reported places us on the alert for the reference genes estimated to be constitutive to quantitatively assess gene transcripts.
Sarmiento
J
J
Instituto de Bioquímica, Universidad Austral de Chile, Valdivia, Chile.
Leal
S
S
Quezada
C
C
Kausel
G
G
Figueroa
J
J
Inés Vera
M
M
Krauskopf
M
M
D000199:Actins / Q000235:genetics*; D000222:Adaptation, Physiological; D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000502:physiology*; D017931:DNA Primers; D017403:In Situ Hybridization; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012335:RNA, Ribosomal / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000502:physiology*
D000199:Actins; D017931:DNA Primers; D012333:RNA, Messenger; D012335:RNA, Ribosomal