Transcription activation by a variety of AraC/XylS family activators does not depend on the class II-specific activation determinant in the N-terminal domain of the RNA polymerase alpha subunit.
The N-terminal domain of the RNA polymerase alpha subunit (alpha-NTD) was tested for a role in transcription activation by a variety of AraC/XylS family members. Based on substitutions at residues 162 to 165 and an extensive genetic screen we conclude that alpha-NTD is not an activation target for these activators.
Egan
S M
SM
Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas 66045, USA. sme@ukans.edu
Pease
A J
AJ
Lang
J
J
Li
X
X
Rao
V
V
Gillette
W K
WK
Ruiz
R
R
Ramos
J L
JL
Wolf
R E
RE
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D003561:Cytarabine / Q000378:metabolism*; D004268:DNA-Binding Proteins; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins; D009154:Mutation; D016958:Pseudomonas putida / Q000235:genetics / Q000378:metabolism; D012808:Sigma Factor / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D015533:Transcriptional Activation
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D012808:Sigma Factor; D015534:Trans-Activators; D014157:Transcription Factors; C078720:XylS protein, Pseudomonas putida; C069567:sigma factor KatF protein, Bacteria; D003561:Cytarabine; C070725:SoxS protein, E coli; D012321:DNA-Directed RNA Polymerases; C113275:RNA polymerase alpha subunit