Fission yeast retrotransposon Tf1 integration is targeted to 5' ends of open reading frames.
Target site selection of transposable elements is usually not random but involves some specificity for a DNA sequence or a DNA binding host factor. We have investigated the target site selection of the long terminal repeat-containing retrotransposon Tf1 from the fission yeast Schizosaccharomyces pombe. By monitoring induced transposition events we found that Tf1 integration sites were distributed throughout the genome. Mapping these insertions revealed that Tf1 did not integrate into open reading frames, but occurred preferentially in longer intergenic regions with integration biased towards a region 100-420 bp upstream of the translation start site. Northern blot analysis showed that transcription of genes adjacent to Tf1 insertions was not significantly changed.
Behrens
R
R
Imperial Cancer Research Fund, Cell Cycle Laboratory, 44 Lincoln's Inn Fields, London WC2A 3PX, UK. r.behrens@icrf.icnet.uk
Hayles
J
J
Nurse
P
P
D001665:Binding Sites; D015139:Blotting, Southern; D018797:Cell Cycle Proteins; D004271:DNA, Fungal / Q000235:genetics*; D004274:DNA, Recombinant; D016254:Mutagenesis, Insertional; D009154:Mutation; D009687:Nuclear Proteins; D016366:Open Reading Frames / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D018626:Retroelements; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012568:Schizosaccharomyces / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins; D020687:cdc25 Phosphatases / Q000235:genetics
D018797:Cell Cycle Proteins; D004271:DNA, Fungal; D004274:DNA, Recombinant; D009687:Nuclear Proteins; D018626:Retroelements; D029702:Schizosaccharomyces pombe Proteins; C068689:wee1 protein, S pombe; D011505:Protein-Tyrosine Kinases; D020687:cdc25 Phosphatases