Cytoskeleton actin changes in IL-2 activated cells.
In the present study we analysed the changes in cytoskeleton actin in lymphoid cells following IL-2 activation and during cell interactions by means of light and electron microscopy, immunofluorescence and molecular analysis. By morphological analysis we observed a higher fluorescence in the activated cells than in the quiescent ones with no modifications in the cytoskeleton pattern comparing activated to resting cells. The results of molecular analysis indicate that, after IL-2 activation, there is a reorganisation of the actin component of the cell cytoskeleton accompanied by the differential expression of the corresponding genes. A future study will be extended to the analysis of others components of the cytoskeleton network.
Capelli
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E
Dipartimento di Genetica e Microbiologia, Università di Pavia, Italy. Capelli@ipvgen.unipv.it
Nano
R
R
Panelli
S
S
Sciola
L
L
Spano
A
A
Barni
S
S
D000199:Actins / Q000235:genetics*; D002460:Cell Line; D002478:Cells, Cultured; D018920:Coculture Techniques; D003599:Cytoskeleton / Q000187:drug effects* / Q000648:ultrastructure; D006367:HeLa Cells; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D020014:K562 Cells; D015979:Killer Cells, Lymphokine-Activated / Q000166:cytology / Q000502:physiology; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D008113:Liver Neoplasms; D011994:Recombinant Proteins / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000187:drug effects; D014407:Tumor Cells, Cultured
D000199:Actins; D007376:Interleukin-2; D011994:Recombinant Proteins