title: An efficient solid-phase strategy for the construction of chemokines.
abstract: Synthesis of chemokines via stepwise SPPS approaches has been shown to be a challenge. Herein, a complete study of different coupling methods, solvents and temperature combined with a continuous-flow synthesizer equipped with feedback monitoring was carried out. The results from this study indicate that this family of molecules can be prepared using an Fmoc/Bu(t) chemical approach and provide a general method to apply for the elongation of other difficult sequences.
authors.affiliation: PerSeptive Biosystems Inc., Framingham, MA, USA.
authors.forename: J F
authors.initials: JF
authors.lastname: McNamara
authors.forename: H
authors.initials: H
authors.lastname: Lombardo
authors.forename: S K
authors.initials: SK
authors.lastname: Pillai
authors.forename: I
authors.initials: I
authors.lastname: Jensen
authors.forename: F
authors.initials: F
authors.lastname: Albericio
authors.forename: S A
authors.initials: SA
authors.lastname: Kates
mesh_terms: D000595:Amino Acid Sequence; D001671:Biochemistry / Q000379:methods*; D018946:Chemokine CCL5 / Q000138:chemical synthesis; D018925:Chemokines / Q000138:chemical synthesis*; D008969:Molecular Sequence Data
chemical_list: D018946:Chemokine CCL5; D018925:Chemokines