High-level fluoroquinolone-resistant clinical isolates of Escherichia coli overproduce multidrug efflux protein AcrA.
Immunoblotting with antibody against AcrA, an obligatory component of the AcrAB multidrug efflux system, showed that this protein was overexpressed by >/=170% in 9 of 10 clinical isolates of Esherichia coli with high-level ciprofloxacin resistance (MICs, >/=32 microg/ml) but not in any of the 15 isolates for which the MIC was </=1 microg/ml.
Mazzariol
A
A
Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3206, USA.
Tokue
Y
Y
Kanegawa
T M
TM
Cornaglia
G
G
Nikaido
H
H
D000890:Anti-Infective Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000276:immunology / Q000502:physiology; D001692:Biological Transport / Q000502:physiology; D002939:Ciprofloxacin / Q000494:pharmacology*; D004352:Drug Resistance, Microbial / Q000502:physiology; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism; D029968:Escherichia coli Proteins; D006801:Humans; D008074:Lipoproteins / Q000096:biosynthesis* / Q000276:immunology / Q000502:physiology; D026901:Membrane Transport Proteins; D008826:Microbial Sensitivity Tests
C083497:AcrA protein, E coli; D000890:Anti-Infective Agents; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D008074:Lipoproteins; D026901:Membrane Transport Proteins; D002939:Ciprofloxacin