Sendai virus binds to a dispersed population of NBD-GD1a.
Receptor aggregation is believed to be an important step in the attachment of membrane enveloped virus' to target cell membranes. A likely receptor for Sendai virus is the ganglioside GD1a. In this work we have studied the membrane diffusion of the fluorescent ganglioside NBD-GD1a on the surface of CV-1 cells with standard photobleaching techniques. Using confocal laser scanning microscopy (CLSM) and Image Correlation Spectroscopy (ICS) NBD-GD1a is shown to exist in at least two populations: dispersed and aggregated. By quantifying the distribution of NBD-GD1a pre- and post-incubation with Sendai virus it is shown that the virus induces a dose-dependent clustering of NBD-GD1a. Image cross-correlation spectroscopy (ICCS) is used to further quantitatively characterize this clustering by demonstrating that it occurs due to binding of virus to the dispersed population of NBD-GD1a.
Rocheleau
J V
JV
Department of Biochemistry, The University of Western Ontario, London, Canada.
Petersen
N O
NO
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D004058:Diffusion / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism / Q000821:virology; D005456:Fluorescent Dyes / Q000378:metabolism; D005732:Gangliosides / Q000378:metabolism*; D000882:Haplorhini; D018613:Microscopy, Confocal; D011940:Receptor Aggregation / Q000187:drug effects; D011991:Receptors, Virus / Q000378:metabolism*; D018108:Respirovirus / Q000378:metabolism*; D019810:Sodium Azide / Q000494:pharmacology; D013057:Spectrum Analysis; D013696:Temperature
D005456:Fluorescent Dyes; D005732:Gangliosides; D011991:Receptors, Virus; C021298:ganglioside, GD1a; D019810:Sodium Azide