title: In vitro assessment of Metarhizium anisopliae isolates to control the cattle tick Boophilus microplus.
abstract: Metarhizium anisopliae is a filamentous fungus used for tick control. The in vitro effects of 12 M. anisopliae isolates on engorged Boophilus microplus females were analysed. The most pathogenic isolate (E6S1) caused a 100% death rate when 10(7) spores/ml were used to infect ticks. Isolates of M. anisopliae taken from experimentally infected ticks proved to be more pathogenic than fungus maintained on culture media. A comparison between dsRNA mycovirus-free and infected M. anisopliae isolates suggested that, in general, virus free isolates were more infective. The results showed that the biological control of B. microplus by M. anisopliae infection might constitute an additional method to integrated tick control management.
authors.affiliation: Programa de Pós-graduação em Biologia Celular e Molecular (PPGBCM/CBiot), Universidade Federal do Rio Grande do Sul, Campus do Vale, P.O. Box 15005, 91501-970, Porto Alegre, Brazil.
authors.forename: A P
authors.initials: AP
authors.lastname: Frazzon
authors.forename: I
authors.initials: I
authors.lastname: da Silva Vaz Junior
authors.forename: A
authors.initials: A
authors.lastname: Masuda
authors.forename: A
authors.initials: A
authors.lastname: Schrank
authors.forename: M H
authors.initials: MH
authors.lastname: Vainstein
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology / Q000517:prevention & control*; D005260:Female; D003904:Mitosporic Fungi / Q000472:pathogenicity*; D010572:Pest Control, Biological; D013172:Spores, Fungal / Q000472:pathogenicity; D013984:Tick Infestations / Q000517:prevention & control / Q000662:veterinary*; D013987:Ticks / Q000382:microbiology* / Q000502:physiology