Infection and activation of airway epithelial cells by Chlamydia pneumoniae.
The activation of primary human airway epithelial cells (HAECs) and of the bronchial epithelial cell line BEAS-2B by Chlamydia pneumoniae, an important respiratory pathogen, was characterized. A time-dependent enhanced release of interleukin (IL)-8 and prostaglandin-E(2) and an increased expression of the epithelial adhesion molecule intercellular adhesion molecule-1 (ICAM-1), followed by subsequent transepithelial migration of polymorphonuclear neutrophils (PMN), were also demonstrated. The transepithelial PMN migration could be blocked by an anti-ICAM-1 monoclonal antibody (MAb) but not by MAbs against IL-8. In addition, there was an enhanced C. pneumoniae-mediated activation of NF-kappaB within 30-60 min in HAECs and BEAS-2B, which was followed by increases in mRNA synthesis of IL-8, ICAM-1, and cyclooxygenase-2, with maximal effects occurring 2 h after infection. Thus, C. pneumoniae infects and activates HAECs and BEAS-2B and therefore may be able to trigger a cascade of pro- and anti-inflammatory reactions during chlamydial infections.
Jahn
H U
HU
Department of Internal Medicine/Infectious Diseases, Charité, Medical School of Humboldt University, Berlin, Germany.
Krüll
M
M
Wuppermann
F N
FN
Klucken
A C
AC
Rosseau
S
S
Seybold
J
J
Hegemann
J H
JH
Jantos
C A
CA
Suttorp
N
N
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001980:Bronchi; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D016993:Chlamydophila pneumoniae; D051546:Cyclooxygenase 2; D004847:Epithelial Cells / Q000276:immunology / Q000382:microbiology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis / Q000276:immunology; D016209:Interleukin-8 / Q000032:analysis / Q000276:immunology; D019746:Intramolecular Oxidoreductases / Q000032:analysis; D007527:Isoenzymes / Q000032:analysis; D008565:Membrane Proteins; D016328:NF-kappa B / Q000032:analysis; D009504:Neutrophils / Q000276:immunology; D000071876:Prostaglandin-E Synthases; D011451:Prostaglandin-Endoperoxide Synthases / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D015398:Signal Transduction; D013997:Time Factors
D000911:Antibodies, Monoclonal; D016209:Interleukin-8; D007527:Isoenzymes; D008565:Membrane Proteins; D016328:NF-kappa B; D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases; D019746:Intramolecular Oxidoreductases; D000071876:Prostaglandin-E Synthases