title: Sec61p is the main ribosome receptor in the endoplasmic reticulum of Saccharomyces cerevisiae.
abstract: A characteristic feature of the co-translational protein translocation into the endoplasmic reticulum (ER) is the tight association of the translating ribosomes with the translocation sites in the membrane. Biochemical analyses identified the Sec61 complex as the main ribosome receptor in the ER of mammalian cells. Similar experiments using purified homologues from the yeast Saccharomyces cerevisiae, the Sec61p complex and the Ssh1p complex, respectively, demonstrated that they bind ribosomes with an affinity similar to that of the mammalian Sec61 complex. However, these studies did not exclude the presence of other proteins that may form abundant ribosome binding sites in the yeast ER. We now show here that similar to the situation found in mammals in the yeast Saccharomyces cerevisiae the two Sec61-homologues Sec61p and Ssh1p are essential for the formation of high-affinity ribosome binding sites in the ER membrane. The number of binding sites formed by Ssh1p under standard growth conditions is at least 4 times less than those formed by Sec61p.
authors.affiliation: Abteilung Biochemie II, Zentrum Biochemie und Molekulare Zellbiologie, Georg-August-Universität Göttingen, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Prinz
authors.forename: E
authors.initials: E
authors.lastname: Hartmann
authors.forename: K U
authors.initials: KU
authors.lastname: Kalies
mesh_terms: D004721:Endoplasmic Reticulum / Q000378:metabolism* / Q000648:ultrastructure; D005656:Fungal Proteins / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D026901:Membrane Transport Proteins; D008861:Microsomes / Q000378:metabolism / Q000648:ultrastructure; D014176:Protein Biosynthesis; D012270:Ribosomes / Q000378:metabolism* / Q000648:ultrastructure; D000069816:SEC Translocation Channels; D012441:Saccharomyces cerevisiae / Q000378:metabolism* / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D005656:Fungal Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D000069816:SEC Translocation Channels; C499383:SEC61 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins