The effect of in ovo or day-of-hatch subcutaneous antibiotic administration on competitive exclusion culture (PREEMPT) establishment in neonatal chickens.
The effect of in ovo or day-of-hatch subcutaneous antibiotic administration on the detection of antibiotic residues in yolk sac or blood serum samples and the potential for observed residues to interfere with competitive exclusion (CE) culture establishment was compared in three experiments. The in ovo or subcutaneous administration of gentamicin sulfate or ceftiofur sodium was associated with detectable levels of antibiotic residues in yolk sac or blood serum samples in Experiment 1. Further, the ability to detect antibiotic residues in day-of-hatch chicks was associated with reduced levels of CE culture establishment when cecal propionate level, an indicator of PREEMPT establishment, was determined following PREEMPT application by oral gavage on the day of hatch in Experiments 1 and 2. Restricting chicks from feed, as opposed to providing access ad libitum to a starter ration, for 6 h immediately following administration of PREEMPT improved (P < 0.05) CE culture establishment in Experiment 2 in nonantibiotic injected control chicks, but did not affect (P > 0.05) experimental groups receiving either gentamicin sulfate or ceftiofur sodium by either in ovo or subcutaneous routes. The in ovo administration of 0.1 or 0.2 mg ceftiofur sodium to individual embryos on Day 18 of embryogenesis in Experiment 3 was associated with marked depressions (P < 0.05) in cecal propionate levels compared with uninjected control chicks. When feed was restricted and the time of PREEMPT administration was delayed for 48 or 72 h posthatch, mean cecal propionate levels in in ovo ceftiofur sodium-injected chicks were not significantly different (P > 0.05) from controls, indicating a time- and feed restriction-associated effect on improving CE culture establishment.
McReynolds
J L
JL
Department of Poultry Science and Veterinary Pathobiology, Texas Agricultural Experiment Station, Texas A&M University, College Station 77843-2472, USA.
Caldwell
D Y
DY
Barnhart
E T
ET
Deloach
J R
JR
McElroy
A P
AP
Moore
R W
RW
Hargis
B M
BM
Caldwell
D J
DJ
D000821:Animal Feed; D000818:Animals; D019072:Antibiotic Prophylaxis / Q000379:methods / Q000662:veterinary*; D001409:Bacillus cereus / Q000187:drug effects; D002432:Cecum; D002511:Cephalosporins / Q000008:administration & dosage* / Q000494:pharmacology; D002642:Chick Embryo; D002645:Chickens / Q000254:growth & development*; D004350:Drug Residues / Q000032:analysis / Q000494:pharmacology; D005232:Fatty Acids, Volatile / Q000032:analysis; D005766:Gastrointestinal Contents / Q000737:chemistry; D005839:Gentamicins / Q000008:administration & dosage* / Q000494:pharmacology; D007279:Injections, Subcutaneous; D007711:Klebsiella pneumoniae / Q000187:drug effects
D002511:Cephalosporins; D005232:Fatty Acids, Volatile; D005839:Gentamicins; C053503:ceftiofur