Preservation of N-methyl-D-aspartate receptor binding sites with age in rat neocortex.
This study used [3H]dizocilpine ([3H]MK-801) binding to examine glycine, polyamine, and zinc subsites of the N-methyl-D-aspartate (NMDA) receptor in well-washed membranes derived from the neocortex of Fischer 344/Norwegian brown rats aged 3, 12, 24 and 37 months. [3H]dizocilpine binding in the presence of 100 microM glutamate was enhanced by the addition of 30 microM glycine. Binding in the presence of both glutamate and glutamate plus glycine were unaffected by age. The competitive polyamine site antagonist arcaine inhibited [3H]dizocilpine binding in a dose-dependent fashion and 50 microM spermidine caused a rightward shift in this dose response curve. IC50 values derived from these plots were not significantly affected by age. Similarly, zinc inhibited binding in a dose-dependent fashion and was also unaffected by age. These data indicate that the NMDA receptor is spared in aging.
Palmer
A M
AM
Department of Psychiatry, University of Pittsburgh School of Medicine, Pennsylvania, USA. a.palmer@vernalis.com
D000375:Aging / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D016291:Dizocilpine Maleate / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D019579:Neocortex / Q000378:metabolism*; D051381:Rats; D011914:Rats, Inbred BN; D011916:Rats, Inbred F344; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D015032:Zinc / Q000494:pharmacology
D016194:Receptors, N-Methyl-D-Aspartate; D016291:Dizocilpine Maleate; D015032:Zinc