title: Mechanical quality of tissue engineered cartilage: results after 6 and 12 weeks in vivo.
abstract: Traumatic events are a primary cause for local lesions of articular cartilage. If treated early, restoration of the initial joint geometry and integrity may be achieved. In large defects, sufficient material is not available to bridge the affected area. Heterologeous transplantation is not well accepted due to the risk of infection and immune response. Alternatives are cartilage-like structures, which may be cultured in vitro and transplanted into the defect site. Critical to the success of these new tissues are their mechanical properties. Goals of this study were to generate a hyaline-like cartilage structure, to evaluate its performance in vivo and to verify that its cellular and material properties meet those of native cartilage. Hyaline-like cartilage specimens were generated in vitro and implanted in the backs of nude mice. Specimens were explanted after 6 and 12 weeks, mechanically tested using an indentation test and histologically examined. In mechanical testing, stiffness and failure load significantly increased between weeks 6 and 12. At 12 weeks, mechanical properties of the hyaline-like cartilage were comparable to those of native nasal septal cartilage. Compared to native articular cartilage, the engineered tissue achieved up to 30-50% in strength and mechanical stiffness. In histological examination, specimens showed neocartilage formation. The mechanical testing procedure proved to be sufficiently sensitive to identify differences in properties between cartilage specimens of different origin and at different stages of healing. As an adjunct to histological analysis, mechanical testing may be a valuable tool for judging the utility of engineered cartilage prior to a broad clinical usage.
authors.affiliation: Charité, Campus Virchow-Klinikum, Humboldt University of Berlin, Augustenburger Platz 1, D-13353 Berlin, Germany. georg.duda@charite.de
authors.forename: G N
authors.initials: GN
authors.lastname: Duda
authors.forename: A
authors.initials: A
authors.lastname: Haisch
authors.forename: M
authors.initials: M
authors.lastname: Endres
authors.forename: C
authors.initials: C
authors.lastname: Gebert
authors.forename: D
authors.initials: D
authors.lastname: Schroeder
authors.forename: J E
authors.initials: JE
authors.lastname: Hoffmann
authors.forename: M
authors.initials: M
authors.lastname: Sittinger
mesh_terms: D000328:Adult; D000818:Animals; D001696:Biomechanical Phenomena; D001698:Biomedical Engineering; D002356:Cartilage / Q000166:cytology / Q000502:physiology*; D019902:Chondrocytes / Q000502:physiology; D015718:Fibrin Tissue Adhesive; D006801:Humans; D008297:Male; D008422:Materials Testing; D009300:Nasal Septum / Q000502:physiology; D051381:Rats; D011923:Rats, Nude; D013314:Stress, Mechanical; D014014:Tissue Adhesives
chemical_list: D015718:Fibrin Tissue Adhesive; D014014:Tissue Adhesives