title: Quantitative PCR analysis of different splice forms of NFAT5 revealed specific gene expression in fetal and adult brain.
abstract: To investigate sequences or mutations associated with neurodegenerative disorders, we performed analyses for the NFAT5 gene, which is located in the candidate region for the autosomal dominantly inherited spinocerebellar ataxia type 4 (SCA4). PCR based expression analyses detected NFAT5 transcripts with alternative splicing in 27 fetal and adult human tissues. Interestingly, by using quantitative methods on cDNA from fetal and adult human brain a significant difference at the expression level for one splice form could be shown.
authors.affiliation: Universität Lübeck, Institut für Humangenetik, Ratzeburger Allee 160, D-23538, Lübeck, Germany. dalski@medinf.mu-luebeck.de
authors.forename: A
authors.initials: A
authors.lastname: Dalski
authors.forename: H J
authors.initials: HJ
authors.lastname: Wagner
authors.forename: E
authors.initials: E
authors.lastname: Schwinger
authors.forename: C
authors.initials: C
authors.lastname: Zühlke
mesh_terms: D000328:Adult; D017398:Alternative Splicing / Q000235:genetics*; D001923:Brain Chemistry / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D005091:Exons; D005333:Fetus; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D050778:NFATC Transcription Factors; D016133:Polymerase Chain Reaction / Q000379:methods; D022821:RNA Splice Sites; D020754:Spinocerebellar Ataxias / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D050778:NFATC Transcription Factors; D022821:RNA Splice Sites; D014157:Transcription Factors