title: Changes in embryonic cell fate produced by expression of an endodermal transcription factor, Xsox17.
abstract: Many molecules induce the ectopic expression of tissue-specific genes in Xenopus embryos. Conversely, interfering with their activity disrupts patterns of gene expression, implicating them in normal development. Does this mean that they control cell fate (i.e. position, as well as differentiation)? Xsox17alpha and beta can induce ectopic expression of endodermal markers; inhibiting their function suppresses expression of endodermal marker genes in the developing gut (Cell 91 (1997) 397). Here we show the effect of these manipulations on cell lineage. Expressing Xsox17 in a cells normally fated to become ectoderm causes their descendants either to relocate into the embryonic gut or to die at a late developmental stage. Conversely, disrupting Xsox17 activity in cells normally fated to be endodermal causes them to enter mesodermal and ectodermal lineages.
authors.affiliation: Department of Biological Sciences, University of Warwick, CV4 7AL, Coventry, UK.
authors.forename: D
authors.initials: D
authors.lastname: Clements
authors.forename: H R
authors.initials: HR
authors.lastname: Woodland
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D019070:Cell Lineage; D004268:DNA-Binding Proteins; D004475:Ectoderm / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D004707:Endoderm / Q000378:metabolism*; D006609:High Mobility Group Proteins; D017403:In Situ Hybridization; D008648:Mesoderm / Q000378:metabolism*; D008954:Models, Biological; D011506:Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D055760:SOXF Transcription Factors; D014157:Transcription Factors; D014158:Transcription, Genetic; D029867:Xenopus Proteins; D014982:Xenopus laevis / Q000196:embryology; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D006609:High Mobility Group Proteins; D011506:Proteins; D012333:RNA, Messenger; D055760:SOXF Transcription Factors; D014157:Transcription Factors; D029867:Xenopus Proteins; C491602:sox17a protein, Xenopus; C491601:sox17b.1 protein, Xenopus; D001616:beta-Galactosidase