A sequence-ready map for human chromosome 12q15-21.
Construction of sequence-ready clone map is an essential step toward sequencing the human genome. We chose a region that is frequently amplified in liposarcoma between D12S350 and D12S106 in chromosome 12q15-21 to build a PAC/BAC clone contig map. This region was spanned by 4 YACs and contained 30 STS on the YAC and radiation hybrid (RH) framework maps, providing an average STS spacing of 160 kb if each YAC is approximately 1.2 Mb in size. To convert a STS-based YAC map to a STS-based contig map of bacterial clones, 22 non-polymorphic STS markers were used as probes to screen the high density gridded arrays of PAC and BAC clones by filter hybridizations, followed by assembly of clones into contigs by marker content. Contigs have been extended and joined by direct end sequencing of appropriate clones, generating new STSs and rescreening the library as necessary. Using these approaches, we have constructed 5 contigs covering the region with the largest single contig being 1.4 Mb and a final size estimation of 3.6 Mb. The map is comprised of 17 YACs, 187 PACs, 160 BACs, and 17 cosmids; onto this, 6 polymorphic, 97 non-polymorphic, 24 ESTs, and 4 gene-based markers are now placed in a unique order, providing an average resolution of approximately 28 kb. Of a total of 131 markers, 97 were developed in the present study. The sequence-ready map should provide a framework to generate complete DNA sequence and ultimately gene map of this segment of chromosome 12.
Lee
S G
SG
Asan Institute for Life Sciences, University of Ulsan College of Medicine, Seoul, Korea.
Cho
K A
KA
Choi
Y H
YH
Montgomery
K
K
Lee
E
E
Miller
A
A
Kucherlapati
R
R
Song
K
K
D001483:Base Sequence; D002874:Chromosome Mapping; D022201:Chromosomes, Artificial; D022202:Chromosomes, Artificial, Bacterial; D018244:Chromosomes, Artificial, Yeast; D002884:Chromosomes, Human, Pair 15; D003360:Cosmids; D015894:Genome, Human; D006801:Humans; D008080:Liposarcoma / Q000235:genetics; D008969:Molecular Sequence Data