Cytogenetic studies of three triazine herbicides. II. In vivo micronucleus studies in mouse bone marrow.
Atrazine, simazine, and cyanazine are widely used preemergence and postemergence triazine herbicides that have made their way into the potable water supply of many agricultural communities. Although there are several contradictory genotoxicity studies in the literature, our previous in vitro studies with human lymphocytes showed that atrazine, simazine, and cyanazine did not induce sister chromatid exchanges (SCEs) or chromosome aberrations (CAs) up to the limits of solubility in aqueous medium using 0.5% dimethyl sulfoxide. To expand upon these results and to ensure that our in vitro findings could be replicated in an in vivo system, mice were treated with each triazine by two intraperitoneal injections, 24h apart. The animals were sacrificed and the bone marrow removed for micronucleus (MN) analysis, 24h after the last injection. Two to four independent trials were performed for MN analysis in polychromatic erythrocytes, and in some trials the spleen was removed, cultured, and analyzed for SCEs and CAs. None of the triazines investigated induced MN in the bone marrow, even at doses that caused significant bone marrow suppression and/or death. These results indicate that atrazine, simazine, and cyanazine are not genotoxic as measured by the bone marrow MN assay in mice following high dose exposures.
Kligerman
A D
AD
Environmental Carcinogenesis Division, U.S. Environmental Protection Agency, Research Triangle Park, NC 27711, USA. klingerman.andrew@epa.gov
Doerr
C L
CL
Tennant
A H
AH
Peng
B
B
D000818:Animals; D001280:Atrazine / Q000008:administration & dosage / Q000633:toxicity*; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000473:pathology; D002478:Cells, Cultured; D004900:Erythroblasts / Q000187:drug effects / Q000473:pathology; D005260:Female; D006540:Herbicides / Q000008:administration & dosage / Q000633:toxicity*; D007274:Injections, Intraperitoneal; D051379:Mice; D008810:Mice, Inbred C57BL; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects; D015162:Micronucleus Tests; D009153:Mutagens / Q000008:administration & dosage / Q000633:toxicity*; D012839:Simazine / Q000008:administration & dosage / Q000633:toxicity*; D013154:Spleen / Q000187:drug effects / Q000473:pathology; D014227:Triazines / Q000008:administration & dosage / Q000633:toxicity*
D006540:Herbicides; D009153:Mutagens; D014227:Triazines; D001280:Atrazine; D012839:Simazine; C007168:cyanazine