Crystallization and preliminary X-ray analysis of endopolygalacturonase SE1 from Trichosporon penicillatum.
Endopolygalacturonase SE1 from Trichosporon penicillatum has been crystallized by the hanging-drop method of vapour diffusion using ammonium sulfate as a precipitant. The crystals belong to the hexagonal space group P6(1) or P6(5), with unit-cell parameters a = b = 135.0, c = 70.7 A, gamma = 120 degrees. The calculated V(M) based on one molecule per asymmetric unit is 3.09 A(3) Da(-1). A native data set from a crystal has been collected to 2.0 A resolution on a Cu Kalpha rotating-anode X-ray source.
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Research Institute for Advanced Science and Technology, Osaka Prefecture University, Sakai, Osaka 599-8570, Japan.
Tada
T
T
Nakamura
Y
Y
Wada
K
K
Nishimura
K
K
Katsuya
Y
Y
Sawada
M
M
Takao
M
M
Sakai
T
T
D018360:Crystallography, X-Ray; D011096:Polygalacturonase / Q000737:chemistry* / Q000302:isolation & purification; D011487:Protein Conformation; D014250:Trichosporon / Q000201:enzymology*
C418471:endopolygalacturonase SE1; D011096:Polygalacturonase