title: A method for the simulation of normal, carrier and affected controls for PCR-RFLP screening of a genetic disease in dairy cattle.
abstract: A technique is described that may be used to create in vitro mutations in PCR templates to generate affected and carrier controls for diagnostic testing when DNA from such individuals is not easily obtained. The method is demonstrated for a PCR-RFLP diagnostic test of the genetic disorder BLAD (Bovine Leukocyte Adhesion Deficiency).
authors.affiliation: Biotechnology Laboratory, National Dairy Development Board, Anand-388 001, Gujarat, India. pratapm@anand.nddb.ernet.in
authors.forename: P N
authors.initials: PN
authors.lastname: Mukhopadhyaya
authors.forename: H H
authors.initials: HH
authors.lastname: Mehta
authors.forename: R N
authors.initials: RN
authors.lastname: Rathod
mesh_terms: D000818:Animals; D001224:Aspartic Acid / Q000737:chemistry; D018821:CD18 Antigens / Q000235:genetics*; D002417:Cattle; D002418:Cattle Diseases / Q000235:genetics*; D004252:DNA Mutational Analysis; D017931:DNA Primers / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D005820:Genetic Testing / Q000379:methods / Q000662:veterinary*; D005998:Glycine / Q000737:chemistry; D018370:Leukocyte-Adhesion Deficiency Syndrome / Q000235:genetics / Q000662:veterinary*; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D012015:Reference Standards
chemical_list: D018821:CD18 Antigens; D017931:DNA Primers; D001224:Aspartic Acid; D005998:Glycine