title: Liquid chromatographic-tandem mass spectrometric method for the analysis of a neurokinin-1 antagonist and its metabolite using automated solid-phase sample preparation and automated data handling and reporting.
abstract: Pharmacokinetic studies play a vital role during the development of new pharmaceutical substances. Data presented demonstrate an accurate, precise and robust assay for a neurokinin-1 receptor antagonist and its metabolite with HPLC-MS-MS. Sample preparation is performed by solid-phase extraction (SPE) in the 96-well plate format. This process is fully automated with a Tecan Genesis pipetting system using its standard robotic manipulator arm (ROMA). All instruments are fully integrated in a study oriented laboratory information system (LIMS) with an Oracle database that communicates bi-directional with the analytical equipment. Finally, the results are reported by push button operation.
authors.affiliation: Boehringer Ingelheim Pharma KG, Department of Pharmacokinetics and Drug Metabolism, Biberach an der Riss, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Schütze
authors.forename: B
authors.initials: B
authors.lastname: Boss
authors.forename: J
authors.initials: J
authors.lastname: Schmid
mesh_terms: D001331:Automation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D064729:Neurokinin-1 Receptor Antagonists; D004364:Pharmaceutical Preparations / Q000097:blood / Q000652:urine; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D064729:Neurokinin-1 Receptor Antagonists; D004364:Pharmaceutical Preparations