Enhanced production of IL-8 in chronic but not in early ileal lesions of Crohn's disease (CD).
Distinct Th1/Th2 patterns have been observed during the evolution of CD. The aim of this study was to compare neutrophil involvement and IL-8 mRNA and protein expression during early recurrent lesions and chronic phases of CD. Twenty-nine patients with CD having ileocolonic resection with anastomosis were studied. Biopsies were obtained during surgery from the non-inflamed ileal mucosa and from chronic ileal lesions. Endoscopic ileal biopsies were also taken from early recurrent ileal lesions occurring 3 months after surgery. Neutrophil counts were performed and mucosal IL-8 levels were evaluated by competitive reverse transcriptase-polymerase chain reaction and immunohistochemistry. Early recurrent ileal lesions were characterized by low neutrophil counts and IL-8 production at the mRNA and protein levels compared with the ileal chronic lesions. The main cellular sources of IL-8 in the early recurrent lesions were neutrophils, while in chronic lesions the majority of IL-8-stained cells were CD3+ T cells and macrophages. These results confirmed that the nature of the inflammatory infiltrate and the expression of cytokine profiles may differ between the acute and chronic phases of CD.
Brandt
E
E
INSERM U167, Institut Pasteur de Lille, France.
Colombel
J F
JF
Ectors
N
N
Gambiez
L
L
Emilie
D
D
Geboes
K
K
Capron
M
M
Desreumaux
P
P
D000328:Adult; D001483:Base Sequence; D002908:Chronic Disease; D003424:Crohn Disease / Q000209:etiology / Q000276:immunology* / Q000473:pathology; D017931:DNA Primers / Q000235:genetics; D005260:Female; D006801:Humans; D007082:Ileum / Q000276:immunology / Q000473:pathology; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000235:genetics; D007413:Intestinal Mucosa / Q000276:immunology / Q000473:pathology; D008297:Male; D009504:Neutrophils / Q000276:immunology / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013997:Time Factors
D017931:DNA Primers; D016209:Interleukin-8; D012333:RNA, Messenger