title: Structural and serological studies of the related O-specific polysaccharides of Proteus vulgaris O21 and Proteus mirabilis O48 having oligosaccharide-phosphate repeating units.
abstract: The O-specific polysaccharide chains (O-antigens) of the lipopolysaccharides (LPSs) of Proteus mirabilis O48 and Proteus vulgaris O21 were found to have tetrasaccharide and pentasaccharide repeating units, respectively, interlinked by a glycosidic phosphate. Polysaccharides and an oligosaccharide were derived from the LPSs by various degradation procedures and studied by 1H and 13C NMR spectroscopy, including 2D COSY, TOCSY, NOESY, H-detected 1H,13C and 1H,31P HMQC experiments. The following related structures of the repeating units of the O-antigens were established (top: Proteus mirabilis O48; bottom: Proteus vulgaris O21) The O-specific polysaccharide of P. vulgaris O21 has the same structure as that of Hafnia allvei 744 and PCM 1194 [Petersson C., Jachymek, W., Klonowska, A., Lugowski, C., Niedziela, T. & Kenne, L. (1997) Eur. J. Biochem., 245, 668-675], except that the GlcN residue carries the N-acetyl rather than the N-[(R)-3-hydroxybutyryl] group. Serological investigations confirmed the close relatedness of the Proteus and Hafnia O-antigens studied.
authors.affiliation: Department of Immunobiology of Bacteria, Institute of Microbiology and Immunology, University of Lodz, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Bartodziejska
authors.forename: F V
authors.initials: FV
authors.lastname: Toukach
authors.forename: E V
authors.initials: EV
authors.lastname: Vinogradov
authors.forename: S N
authors.initials: SN
authors.lastname: Senchenkova
authors.forename: A S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: A
authors.initials: A
authors.lastname: Ziolkowski
authors.forename: J
authors.initials: J
authors.lastname: Czaja
authors.forename: M B
authors.initials: MB
authors.lastname: Perry
authors.forename: Y A
authors.initials: YA
authors.lastname: Knirel
authors.forename: A
authors.initials: A
authors.lastname: Rozalski
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002240:Carbohydrate Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000097:blood / Q000737:chemistry*; D011134:Polysaccharides / Q000097:blood / Q000737:chemistry*; D011513:Proteus mirabilis / Q000737:chemistry*; D011514:Proteus vulgaris / Q000737:chemistry*; D011817:Rabbits
chemical_list: D019081:O Antigens; D011134:Polysaccharides