Genotyping of clinical and chicken isolates of Campylobacter jejuni and Campylobacter coli.
Genomic DNA from 58 strains of Campylobacter made up of 48 Campylobacter jejuni and ten Campylobacter coli were digested with Sma I and analysed by pulsed-field gel electrophoresis (PFGE). The cleavage of DNA by Sma I gave 22 distinct hybridization patterns. The two Campylobacter species were subtyped by PFGE. The average genomic size for C. jejuni by Sma I digestion was 1.73 Mb, while that of C. coli gave 1.7 Mb. Results from this study indicate that PFGE analysis by Sma I digested genomic DNA provides a reliable means of differentiating between and within species of Campylobacter and provides a practical approach to epidemiological studies of Campylobacter.
Smith
S I
SI
Medical Department 1, Technical University Hospital, Dresden, Germany.
Olukoya
D K
DK
Fox
A J
AJ
Coker
A O
AO
D000818:Animals; D002169:Campylobacter Infections / Q000235:genetics*; D017000:Campylobacter coli / Q000235:genetics*; D016123:Campylobacter jejuni / Q000235:genetics*; D002645:Chickens / Q000235:genetics / Q000382:microbiology*; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006801:Humans; D015183:Restriction Mapping; D012703:Serotyping / Q000706:statistics & numerical data