[Pentoxifylline and verapamil influence on the phagocytosis of peritoneal macrophages from women with endometriosis].
OBJECTIVE
Disturbances in topical immunological response during endometriosis, cell and humoral, is of particular interest nowadays. Increase in the number and activity of peritoneal macrophages depends to a large extent on calcium concentration. Pentoxifylline and verapamil are both known for their ability to regulate calcium homeostasis.


METHODS
Evaluation of the pentoxifilline and verapamil influence on the phagocytic activity of the peritoneal macrophages from women with endometriosis.


METHODS
Incubation of the peritoneal fluid with latex in four sets: 1) control with medium for 30 minutes; 2) with pentoxyfilline (3.8 mmol/l) for 30 minutes; 3) with verapamil 0.4 mmol/1; 4) with pentoxyfilline and verapamil together. Percentage of phagocyting cells, phagocytosis index and a score of phagocyting activity was calculated after 30 minutes incubation. Verapamil showed to increase all parameters of phagocytosis in comparison with control.


CONCLUSIONS
Verapamil is effective reagent in activity of peritoneal fluid macrophages. Further studies on larger group of patients are necessary to see if the changes are statistically relevant.
Szamatowicz
J
J
Klinki Ginekologii AM w Białymstoku.
Laudański
P
P
Czygier
M
M
D000328:Adult; D002121:Calcium Channel Blockers / Q000008:administration & dosage / Q000494:pharmacology*; D004715:Endometriosis / Q000378:metabolism*; D005260:Female; D006801:Humans; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D010431:Pentoxifylline / Q000008:administration & dosage / Q000494:pharmacology*; D010587:Phagocytosis / Q000187:drug effects*; D010726:Phosphodiesterase Inhibitors / Q000008:administration & dosage / Q000494:pharmacology*; D014700:Verapamil / Q000008:administration & dosage / Q000494:pharmacology*
D002121:Calcium Channel Blockers; D010726:Phosphodiesterase Inhibitors; D014700:Verapamil; D010431:Pentoxifylline