Cisplatin and UV radiation induce activation of the stress-activated protein kinase p38gamma in human melanoma cells.
The p38 alpha mitogen-activated protein kinase has been implicated in the cellular response to genotoxic agents. Here we show that another p38 family member is also activated in response to cisplatin exposure in human melanoma cells. We identified this isoform as p38gamma based on its recognition by specific antibodies and because, in contrast to p38alpha, its activity was not affected by SB203580. We also found that etoposide caused a much more discrete phosphorylation of both p38alpha and p38gamma than either cisplatin or UV treatment. These results indicate that genotoxic stresses activate several p38 isoforms whose implication in the cellular response might depend on the type of DNA damage.
Pillaire
M J
MJ
Laboratoire de Biologie Cellulaire et Moléculaire du Contrôle de la Prolifération, UMR 5088 CNRS, Université Paul Sabatier, 118 route de Narbonne, Toulouse Cedex, 31062, France.
Nebreda
A R
AR
Darbon
J M
JM
D002945:Cisplatin / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects / Q000528:radiation effects; D005047:Etoposide / Q000494:pharmacology; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D008545:Melanoma; D048291:Mitogen-Activated Protein Kinase 12; D020928:Mitogen-Activated Protein Kinases / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D010766:Phosphorylation; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays; D048051:p38 Mitogen-Activated Protein Kinases
D007527:Isoenzymes; D005047:Etoposide; D048291:Mitogen-Activated Protein Kinase 12; D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases; D002945:Cisplatin