Dissolution profiles of principal ingredients in Kampo medicinal powders by high-performance liquid chromatography.
We describe here a method using HPLC for the simultaneous determination of albiflorin, paeoniflorin, glycyrrhizin and six flavanone glycosides (liquiritin, liquiritin apioside, naringin, neohesperidin, hesperidin and narirutin) in the Kampo medicines, Shigyaku-san and Haino-san. All nine components were separated in less than 40 min by linear gradient elution using a mobile phase containing aqueous phosphoric acid and acetonitrile. The dissolution of these components from powders of Shigyaku-san in aqueous solution at pH 1.80, 4.08 and 6.89 was examined. All of the components except glycyrrhizin were dissolved entirely within 5 min regardless of pH. Dissolution of glycyrrhizin was dependent on the pH of the aqueous solution, and increased with increasing pH.
Okamura
N
N
Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Hiroshima, Japan. okamura@fupharm.fukuyama-u.ac.jp
Maki
T
T
Ishida
S
S
Uraguchi
C
C
Onishi
Y
Y
Sadasue
E
E
Tsuruta
Y
Y
Yagi
A
A
D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D020835:Medicine, Kampo; D011208:Powders; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet
D004365:Drugs, Chinese Herbal; D011208:Powders