Surface-enhanced Raman scattering: a structure-specific detection method for capillary electrophoresis.
A new approach to detecting capillary electrophoresis (CE) eluent components by interfacing CE with a surface-enhanced Raman scattering (SERS) system is described. In this approach, CE-based separation of a mixture of trans-1,2-bis(4-pyridyl)ethylene and N,N-dimethyl-4-nitrosoaniline has been detected by SERS in a postcolumn geometry. The retention time obtained from SERS corresponds well with that from conventional UV-visible detection. Meanwhile, CE eluants are identified by their characteristic vibrational spectra, demonstrating the validity of SERS as a structure-specific detection method for CE. In addition, the ability to monitor SERS intensity changes at molecule-specific frequencies makes selective detection of individual analytes possible, even when separation is incomplete. Finally, CE-SERS is evaluated for separation of amino acids (tyrosine and tryptophan) and environmental pollutants (chlorophenol mixtures).
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Department of Chemistry, Pennsylvania State University, University Park 16802-6300, USA.
Natan
M J
MJ
Keating
C D
CD
D000814:Aniline Compounds / Q000032:analysis; D002733:Chlorophenols; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D009603:Nitroso Compounds / Q000032:analysis; D011725:Pyridines / Q000032:analysis; D012834:Silver; D013053:Spectrophotometry; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*; D014364:Tryptophan / Q000032:analysis; D014443:Tyrosine / Q000032:analysis
D000814:Aniline Compounds; D002733:Chlorophenols; D009603:Nitroso Compounds; D011725:Pyridines; C008651:4-nitrosodimethylaniline; D012834:Silver; D014443:Tyrosine; D014364:Tryptophan; C023650:aniline