A legume lectin from the bark of Robinia pseudoacacia crystallizes in two crystal forms: preliminary diffraction analyses.
The A(4) isoform of the bark lectin RPbAI from Robinia pseudoacacia has been crystallized in two different crystal forms. Crystal form I grows in the P2(1) space group with two tetramers in the asymmetric unit, whereas crystal form II grows in the I222 space group with a monomer in the asymmetric unit. Data sets were collected for both crystal forms to resolution limits of 2.55 and 1.81 A, respectively, which will allow successful structure determinations.
Rabijns
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Laboratorium voor Analytische Chemie en Medicinale Fysicochemie, Faculteit Farmaceutische Wetenschappen, K. U. Leuven, Van Evenstraat 4, B-3000 Leuven, Belgium.
Verboven
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C
Van Damme
E J
EJ
Peumans
W J
WJ
De Ranter
C J
CJ
D003460:Crystallization; D007887:Fabaceae / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry* / Q000302:isolation & purification; D037121:Plant Lectins; D010946:Plants, Medicinal; D011108:Polymers / Q000737:chemistry; D011487:Protein Conformation; D014961:X-Ray Diffraction
D037102:Lectins; D037121:Plant Lectins; D011108:Polymers; C047720:Robinia lectin