title: The endothelin-2/vasoactive intestinal contractor gene: expression and promoter activity in PC12 rat pheochromocytoma cells.
abstract: In order to understand the physiological roles of vasoactive intestinal contractor (VIC)/endothelin-2 (ET-2), we examined the expression of this peptide by specific reverse transcriptase polymerase chain reaction (RT-PCR) analysis and found that PC12 rat pheochromocytoma cells express the VIC gene. The 5'-flanking 1.0 kilo base pair (kb) region of the mouse VIC gene is sufficient to express a secreted alkaline phosphatase (SEAP) reporter gene in transiently transfected PC12 cells. The 1.0 kb promoter region may contain cis-acting elements that determine the rate of the VIC gene transcription in PC12 cells.
authors.affiliation: Biosignaling Department, National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology, Tsukuba, Ibaraki, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Saida
authors.forename: T
authors.initials: T
authors.lastname: Uchide
authors.forename: A
authors.initials: A
authors.lastname: Usui
authors.forename: X D
authors.initials: XD
authors.lastname: Gao
authors.forename: N
authors.initials: N
authors.lastname: Tomizuka
authors.forename: S
authors.initials: S
authors.lastname: Oka
authors.forename: H
authors.initials: H
authors.lastname: Masuda
mesh_terms: D000818:Animals; D019333:Endothelin-2 / Q000235:genetics*; D017930:Genes, Reporter; D036341:Intercellular Signaling Peptides and Proteins; D016716:PC12 Cells; D010455:Peptides / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D019333:Endothelin-2; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D012333:RNA, Messenger; C059568:vasoactive intestinal constrictor