Regulation of rat hippocampal neural cadherin in the kainic acid induced seizures.
Regulation of neural (N-) cadherin expression in the hippocampus was examined by in situ hybridization and immunohistochemistry methods in the rat model of kainic acid (KA) induced seizures. After 12 and 24 h of KA administration, mRNA expression level of N-cadherin decreased in the hippocampal CA1 and CA3 area in parallel with decrease of the number of neural cells. In contrast, after 48 h and 7 days, mRNA expression level recovered partially, although the number of neural cells remained small. In addition, immunohistochemical staining indicated that N-cadherin protein expression of survived neurons increased significantly after 48 h of KA administration. These results indicated that N-cadherin might be involved in neuronal reconstruction at the hippocampus.
Fujita
M
M
Department of Neurosurgery, Nagoya City University Medical School, 1 Kawasumi, Mizuho-cho, Mizuho-ku, 467-8601, Nagoya, Japan. masafj@med.nagoya-cu.ac.jp
Aihara
N
N
Yamamoto
M
M
Ueki
T
T
Asai
K
K
Tada
T
T
Kato
T
T
Yamada
K
K
D000818:Animals; D015820:Cadherins / Q000378:metabolism*; D018690:Excitatory Amino Acid Agonists; D006624:Hippocampus / Q000378:metabolism*; D007608:Kainic Acid; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism*
D015820:Cadherins; D018690:Excitatory Amino Acid Agonists; D012333:RNA, Messenger; D007608:Kainic Acid