Immunological properties of Hn-33 purified from type A Clostridium botulinum.
Type A botulinum neurotoxin is produced along with 6-7 neurotoxin associated proteins to form a complex in addition to the neurotoxin. Immunological reactivity of type A botulinum complex and purified hemagglutinin (Fu et al., 1998) to polyclonal antibody raised against the complex have been investigated using ELISA. In competitive ELISA, 50% inhibition of the binding of IgG antibody against complex A is observed at 78 ng/ml of complex and hemagglutinin-33, suggesting that the hemagglutinin-33 accounts for most of the immunogenic response of the complex. Considering the fact that the complex consists of a group of proteins with a cumulative molecular weight of 900 kDa, hemagglutinin-33 may be one of the major immunoreactive proteins present in the type A neurotoxin complex.
Sharma
S K
SK
Department of Chemistry and Biochemistry, and Center for Marine Science and Technology, University of Massachusetts Dartmouth, Dartmouth, MA 02747-2300, USA.
Singh
B R
BR
D000906:Antibodies; D001426:Bacterial Proteins; D001667:Binding, Competitive; D001905:Botulinum Toxins; D019274:Botulinum Toxins, Type A / Q000276:immunology*; D003014:Clostridium botulinum / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D006388:Hemagglutinins / Q000276:immunology* / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000276:immunology
D000906:Antibodies; D001426:Bacterial Proteins; D006388:Hemagglutinins; D007074:Immunoglobulin G; C118973:hemagglutinin 33 protein, Clostridium botulinum; D001905:Botulinum Toxins; D019274:Botulinum Toxins, Type A