title: CpG DNA is an effective oral adjuvant to protein antigens in mice.
abstract: We have previously reported that synthetic oligodeoxynucleotides containing immunostimulatory CpG motifs (CpG ODN) are potent adjuvants to protein administered by intramuscular (IM) injection or intranasal (IN) inhalation to BALB/c mice. Herein, we have evaluated oral delivery of CpG ODN with purified hepatitis B surface antigen (HBsAg) or tetanus toxoid (TT) to determine its potential as an adjuvant to oral vaccines. CpG ODN augmented systemic (IgG in plasma, CTL, T-cell proliferation) and mucosal (IgA in lung, vaginal or gut washes, feces and saliva) immune responses against both antigens. CpG stimulated both T-helper type 1 (Th1) (CTL, IgG2a) and Th2 (IgG1, IgA) responses when delivered orally. Results from this study indicate that stimulatory CpG ODN may be effective as an adjuvant with oral vaccines.
authors: Loeb Health Research Institute at the Ottawa Hospital, 725 Parkdale Avenue, Ont., K1Y 4E9, Ottawa, Canada.
authors: M J
authors: MJ
authors: McCluskie
authors: R D
authors: RD
authors: Weeratna
authors: A M
authors: AM
authors: Krieg
authors: H L
authors: HL
authors: Davis
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage* / Q000235:genetics*; D000284:Administration, Oral; D000818:Animals; D000941:Antigens / Q000008:administration & dosage*; D001483:Base Sequence; D018899:CpG Islands / Q000235:genetics* / Q000276:immunology*; D005260:Female; D006514:Hepatitis B Surface Antigens / Q000008:administration & dosage; D018928:Immunity, Mucosal; D007074:Immunoglobulin G / Q000097:blood; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D009838:Oligodeoxyribonucleotides / Q000235:genetics / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D013745:Tetanus Toxoid / Q000008:administration & dosage; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000235:genetics*
chemical_list: D000276:Adjuvants, Immunologic; D000941:Antigens; D006514:Hepatitis B Surface Antigens; D007074:Immunoglobulin G; D009838:Oligodeoxyribonucleotides; D013745:Tetanus Toxoid; D019444:Vaccines, DNA