Highly reproducible capillary zone electrophoresis of humic acids in cyclodextrin- or oligosaccharide-modified background electrolytes.
Capillary zone electrophoresis has been used for the characterization and separation of humic acids. It was found that addition of saccharides like alpha-, beta-, gamma-cyclodextrins, maltose, hydroxyethylcellulose or dextran sulfate in the background electrolyte (50 mM Na2 B4 O7, pH 9.6) yields better separation patterns and highly reproducible electropherograms. Electropherograms with higher numbers of peaks and high reproducibility were obtained with alpha- and beta-cyclodextrins or with a mixture of alpha- + gamma-cyclodextrin-modified background electrolytes. Separation was carried out with the cathode at the detector end of the column. Adsorption of humic acids to the capillary wall was diminished using an epoxy-coated capillary tube.
Pokorná
L
L
Department of Analytical Chemistry, Faculty of Science, Masaryk University, Brno, Czech Republic.
Pacheco
M L
ML
Havel
J
J
D003505:Cyclodextrins / Q000737:chemistry*; D004573:Electrolytes / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006812:Humic Substances / Q000032:analysis*; D009844:Oligosaccharides / Q000737:chemistry*; D015203:Reproducibility of Results
D003505:Cyclodextrins; D004573:Electrolytes; D006812:Humic Substances; D009844:Oligosaccharides