[The production of a latex immunoglobulin conjugate for the diagnosis of Gardnerella vaginalis].
To obtain an anti-Gadnerella vaginalis latex globulin, specific immunosera to reference strain (ATCC 14018) and to a clinical isolation strain pool were produced. These immunosera were characterized using PAGE-SDS and immunoblotting where a close antigenic relation between the clinical isolation strains and the reference strain was observed. The latex globulin conjugates obtained from these 2 immunosera were evaluated in vitro with a resulting level of detection of 10 ufc/mL of Gardnerella vaginalis. These two conjugates were also evaluated in clinical samples and compared with the 6 vaginalis culture and the criteria considered for diagnosing bacterial vaginosis. The anti-strain pool (II) latex globulin conjugate turned out to be more sensitive and specific than anti-G. vaginalis ATCC strain (I) latex globulin.
Espinosa
I
I
Centro Nacional de Sanidad Agropecuaria, San José de las Lajas, La Habana, Cuba.
Alvarez
E
E
Amaral
C
C
Alonso
M
M
Lorenzo
M
M
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006194:Gardnerella vaginalis / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000032:analysis / Q000302:isolation & purification; D007114:Immunization / Q000379:methods; D015151:Immunoblotting; D007136:Immunoglobulins / Q000302:isolation & purification; D007840:Latex; D007841:Latex Fixation Tests / Q000379:methods; D012756:Sheep; D016585:Vaginosis, Bacterial / Q000175:diagnosis*
D000942:Antigens, Bacterial; D007106:Immune Sera; D007136:Immunoglobulins; D007840:Latex