Cholinergic endings on various neurones containing calcium binding proteins and glutamic acid decarboxylase in the hippocampus of the rat.
Immunohistochemical study of the cholinergic innervation of the hippocampal cells containing glutamic acid decarboxylase (GAD) and calcium binding proteins: parvalbumin (PV), calbindin D28k (CB) and calretinin (CR) was conducted on 5 adult rat brains. Analysis of sections with double immunostaining for vesicular acetylcholine transporter (VAChT; the marker of cholinergic cells, fibres and terminals) and respectively either GAD or PV, CB, CR, using confocal laser-scanning microscope shows that the intensive cholinergic innervations receive GAD, PV and CB-positive hippocampal cells. Cholinergic afferentiations of the CR-positive neurones are considerably fewer.
Ludkiewicz
B
B
Department of Anatomy and Neurobiology, Medical University of Gdańsk, Poland.
Wójcik
S
S
Spodnik
E
E
Domaradzka-Pytel
B
B
Moryś
J
J
D000109:Acetylcholine / Q000032:analysis; D000818:Animals; D064092:Calbindin 1; D064032:Calbindin 2; D064026:Calbindins; D002135:Calcium-Binding Proteins / Q000032:analysis*; D002352:Carrier Proteins / Q000032:analysis*; D005968:Glutamate Decarboxylase / Q000032:analysis*; D006624:Hippocampus / Q000166:cytology* / Q000201:enzymology / Q000648:ultrastructure; D026901:Membrane Transport Proteins; D009411:Nerve Endings / Q000201:enzymology / Q000648:ultrastructure*; D009419:Nerve Tissue Proteins / Q000032:analysis; D009474:Neurons / Q000201:enzymology / Q000648:ultrastructure*; D010320:Parvalbumins / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D064030:S100 Calcium Binding Protein G / Q000032:analysis; D050494:Vesicular Acetylcholine Transport Proteins; D033921:Vesicular Transport Proteins
C576500:Calb1 protein, rat; C576512:Calb2 protein, rat; D064092:Calbindin 1; D064032:Calbindin 2; D064026:Calbindins; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D010320:Parvalbumins; D064030:S100 Calcium Binding Protein G; C493461:Slc18a3 protein, rat; D050494:Vesicular Acetylcholine Transport Proteins; D033921:Vesicular Transport Proteins; D005968:Glutamate Decarboxylase; D000109:Acetylcholine