[Influence of culture conditions on cell surface hydrophobicity of rods of genus Serratia].
The cell surface hydrophobicity (CSH) is a non-specific adhesion factor that is important in the proliferation of microorganisms on solid surfaces. Serratia spp. is a bacterium that has been increasingly implicated as a primary pathogen in numerous human infections, particularly in urinary tract infections. CSH of 60 Serratia spp. strains isolated from clinical materials was evaluated using the ammonium sulfate salt aggregation test. Bacteria were grown for 24 h and 48 h at room temperature (22 degrees C) and 37 degrees C on enrichment broth and agar (Biomed), enrichment agar with 5% human blood and medium composed of agar granulated (Becton Dickinson), neopeptone (Difco) and 1% (v/v) glycerol. CSH was estimated most frequently when the analyzed strains in enrichment broth were cultured. When grown in enrichment broth cells of Serratia spp. at room temperature were more hydrophobic (43% after 24 h and 47% after 48 h) than those at 37 degrees C (30% after 24 h and 33% after 48 h). CSH of the examined Serratia spp. strains were depended on the temperature, time of the culture of bacteria and the kind of media. The influence of the culture conditions on the changes in CSH of the analyzed bacteria may suggest significance of these properties in the pathogenesis of Serratia spp.
Mikucka
A
A
Katedra i Zakład Mikrobiologii Akademii Medycznej w Bydgoszczy.
Gospodarek
E
E
Ulatowska
B
B
D000362:Agar; D001422:Bacterial Adhesion / Q000502:physiology*; D001431:Bacteriological Techniques; D006801:Humans; D012705:Serratia / Q000145:classification / Q000502:physiology*; D013045:Species Specificity; D013499:Surface Properties; D013696:Temperature; D014552:Urinary Tract Infections / Q000382:microbiology
D000362:Agar