Effect of citrate concentration on coagulation test results in dogs.
OBJECTIVE
To determine the effect of citrate concentration (3.2 vs 3.8%) on coagulation tests in dogs.


METHODS
Original study.


METHODS
30 clinically healthy dogs and 12 dogs with hereditary hemostatic disorders.


METHODS
Blood was collected from all dogs directly into collection tubes containing 3.2 or 3.8% buffered citrate. Prothrombin time (PT), activated partial thromboplastin time (aPTT), and fibrinogen concentration were measured by use of 3 clot-detection assay systems (2 mechanical and 1 photo-optic). Factor VIII and factor IX coagulant activities (FVIII:C and FIX:C, respectively) were determined by use of a manual tilt-tube method and a mechanical clot-detection device.


RESULTS
Significant differences were not detected in median PT, fibrinogen concentration, FVIII:C, or FIX:C between 3.2 and 3.8% citrate for any assay system. A significant prolongation in aPTT for 3.2% citrate, compared with 3.8% citrate, was found in 1 mechanical system.


CONCLUSIONS
Citrate concentration does not significantly affect results of most coagulation assays, regardless of assay system. The aPTT was mildly influenced by the citrate concentration, although this was animal-, instrument-, and reagent-dependent. The choice of 3.2 or 3.8% citrate as an anticoagulant for coagulation tests has minimal influence on assay results in healthy dogs or dogs with hereditary hemostatic disorders.
Stokol
T
T
Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
Brooks
M B
MB
Erb
H N
HN
D000818:Animals; D000925:Anticoagulants / Q000097:blood*; D019343:Citric Acid / Q000097:blood*; D020147:Coagulation Protein Disorders / Q000175:diagnosis / Q000662:veterinary; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs / Q000097:blood*; D005164:Factor IX / Q000737:chemistry; D005169:Factor VIII / Q000737:chemistry; D005340:Fibrinogen / Q000032:analysis*; D006467:Hemophilia A / Q000175:diagnosis / Q000662:veterinary; D002836:Hemophilia B / Q000175:diagnosis / Q000662:veterinary; D010314:Partial Thromboplastin Time / Q000662:veterinary*; D011517:Prothrombin Time / Q000662:veterinary*; D014842:von Willebrand Diseases / Q000175:diagnosis / Q000662:veterinary
D000925:Anticoagulants; D019343:Citric Acid; D005169:Factor VIII; D005164:Factor IX; D005340:Fibrinogen