Glial growth factor-2 promotes the survival, migration and bromodeoxyuridine incorporation of mammalian neural crest cells in caudal neural tube explant cultures.
Using an in vitro assay system, we found that GGF-2 increases the number of nascent trunk neural crest cells (NCC) present in the dorsal outgrowth derived from E12 caudal neural tube explants. Data is presented which suggests that this increased outgrowth was due to a combination of GGF-2 mediated effects, including its ability to promote (A) NCC survival by decreasing the percentage of NCC that undergo cell death via a mechanism involving DNA fragmentation, (B) the initial phases of NCC migration, (C) mitosis of peripherally migrating NCC. We also show that GGF-2 can promote the long-term survival of NCC in the absence of the neural tube. An immunohistochemical analysis indicates that NCC express erbB-2 and erbB-4 neuregulin receptors.
Bannerman
P G
PG
Department of Neurology Research, Abramson Pediatric Research Center, Children's Hospital of Philadelphia, 34th & Civic Center Boulevard, Philadelphia, PA 19104, USA. bannerman@email.chop.edu
Puhalla
S
S
Sahai
A
A
Shieh
A
A
Berman
M
M
Pleasure
D
D
D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002490:Central Nervous System / Q000196:embryology*; D046508:Culture Techniques; D006801:Humans; D009419:Nerve Tissue Proteins; D009432:Neural Crest / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D020890:Neuregulin-1 / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism
D001973:Bromodeoxyuridine; D009419:Nerve Tissue Proteins; D020890:Neuregulin-1; D018719:Erb-b2 Receptor Tyrosine Kinases; C094131:NRG1 protein, human; C490580:Nrg1 protein, rat