Chromosome 2 short arm translocations revealed by M-FISH analysis of neuroblastoma cell lines.
METHODS
M-FISH analysis was performed on 18 neuroblastoma cell lines, which were previously studied with cytogenetic, standard FISH and CGH data.


RESULTS
One of the most striking findings of this study was the detection of chromosome 2 short arm rearrangements in 61% of the investigated cell lines. These rearrangements resulted from translocations with various partner chromosomes. All translocations, except one were unbalanced, leading to the consistent gain of chromosome segment 2pter-p22. A cryptic balanced translocation t(2;4) was observed with a breakpoint located in the vicinity of MYCN in cell line NBL-S.


CONCLUSIONS
Combination of M-FISH results together with cytogenetic, standard FISH and CGH data yielded the most comprehensive description of chromosome 2 short arm rearrangements, leading to a consistent gain of chromosome 2 short arm material.
Van Roy
N
N
Center of Medical Genetics, Ghent University Hospital, Gent, Belgium.
Van Limbergen
H
H
Vandesompele
J
J
Van Gele
M
M
Poppe
B
B
Laureys
G
G
De Paepe
A
A
Speleman
F
F
D002648:Child; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D009447:Neuroblastoma / Q000235:genetics*; D014178:Translocation, Genetic / Q000235:genetics*; D014407:Tumor Cells, Cultured