title: Tyrosine hydroxylase-based DNA-vaccination is effective against murine neuroblastoma.
abstract: BACKGROUND
  The disruption of self-tolerance against neuroblastoma is the ultimate goal of an effective DNA-vaccine.
  
  
  METHODS
  Here we demonstrate the induction of a protective immunity against syngeneic murine NXS2 neuroblastoma in A/J mice, following vaccination with tyrosine hydroxylase (TH) derived antigens. Oral gene delivery was accomplished using an attenuated strain of Salmonella typhimurium as a carrier harboring vectors encoding for mTH antigens.
  
  
  RESULTS
  Vaccination was effective in protecting animals from a lethal challenge with wild-type NXS2 tumor cells.
  
  
  CONCLUSIONS
  These results provide the first evidence of the TH self antigen being recognized by T-cells and demonstrate that a TH-based DNA vaccine is a potentially useful immunotherapeutic strategy for neuroblastoma.
authors: The Scripps Research Institute, Department of Immunology, La Jolla, California, USA. holger.lode@charite.de
authors: H N
authors: HN
authors: Lode
authors: U
authors: U
authors: Pertl
authors: R
authors: R
authors: Xiang
authors: G
authors: G
authors: Gaedicke
authors: R A
authors: RA
authors: Reisfeld
mesh_terms: D000818:Animals; D018014:Gene Transfer Techniques; D051379:Mice; D009447:Neuroblastoma / Q000276:immunology / Q000517:prevention & control*; D010957:Plasmids; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics; D019444:Vaccines, DNA
chemical_list: D019444:Vaccines, DNA; D014446:Tyrosine 3-Monooxygenase