NT-4/5 reduces cell death in inner nuclear as well as ganglion cell layers in neonatal rat retina.
Using the TUNEL method, we examined the effect of intraocular NT-4/5 injections on cell death in ganglion and non-ganglion cell layers in 5-day-old rat retinas. NT-4/5 reduced the level of naturally occurring cell death in all retinal layers. Twenty-four hours after superior colliculus (SC) lesions there was a significant increase in the density of TUNEL+ profiles in the RGC layer (6.43/mm2 in normal vs (8.89/mm2 after lesions) which was ameliorated by intraocular NT-4/5 injections (8.79/mm2). Surprisingly, after SC ablation a significant increase in TUNEL+ profiles was also seen in non-ganglion cell layers (52.25/mm2 in normal vs 89.35/mm2 after lesions), mostly in the developing inner nuclear layer. Death in non-ganglion cell layers was also significantly reduced (43.09/mm2) after NT-4/5 eye injections.
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Department of Anatomy and Human Biology, The University of Western Australia, Perth, Australia.
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D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D017209:Apoptosis / Q000187:drug effects*; D005123:Eye; D020287:In Situ Nick-End Labeling; D007267:Injections; D009414:Nerve Growth Factors / Q000008:administration & dosage / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012160:Retina / Q000166:cytology* / Q000187:drug effects; D012165:Retinal Ganglion Cells / Q000187:drug effects*
D009414:Nerve Growth Factors