title: Deletion of tyrosine hydroxylase gene reveals functional interdependence of adrenocortical and chromaffin cell system in vivo.
abstract: Catecholamines are produced in the medulla of the adrenal gland and may participate in the intraglandular regulation of its cortex. We analyzed the adrenal structure and function of albino tyrosine hydroxylase-null (TH-null) mice that are deficient in adrenal catecholamine production. Adrenal catecholamines were markedly reduced, and catecholamine histofluorescence was abrogated in 15-day-old TH-null mice. Chromaffin cell structure was strikingly altered at the ultrastructural level with a depletion of chromaffin vesicles and an increase in rough endoplasmic reticulum compared with wild-type mice. Remaining chromaffin vesicles lined up proximally to the cell membrane in preparation for exocytosis providing a "string-of-pearls" appearance. There was a 5-fold increase in the expression of proenkephalin mRNA (502.8 +/- 142% vs. 100 +/- 17.5%, P = 0.016) and a 2-fold increase in the expression of neuropeptide Y (213.4 +/- 41.2% vs. 100 +/- 59.9%, P = 0.014) in the TH-null animals as determined by quantitative TaqMan (Perkin-Elmer) PCR. Accordingly, immunofluorescence for met-enkephalin and neuropeptide tyrosine in these animals was strongly enhanced. The expression of phenylethanolamine N-methyl transferase and chromogranin B mRNA was similar in TH-null and wild-type mice. In TH-null mice, adrenocortical cells were characterized by an increase in liposomes and by tubular mitochondria with reduced internal membranes, suggesting a hypofunctional state of these steroid-producing cells. In accordance with these findings, plasma corticosterone levels were decreased. Plasma ACTH levels were not significantly different in TH-null mice. In conclusion, both the adrenomedullary and adrenocortical systems demonstrate structural and functional changes in catecholamine-deficient TH-null mice, underscoring the great importance of the functional interdependence of these systems in vivo.
authors: Pediatric and Reproductive Endocrinology Branch, National Institute of Child Health and Human Development, and National Institute of Neurological Diseases and Stroke, National Institutes of Health, Bethesda, MD 20892, USA. bornstes@mail.nih.gov
authors: S R
authors: SR
authors: Bornstein
authors: H
authors: H
authors: Tian
authors: A
authors: A
authors: Haidan
authors: A
authors: A
authors: Böttner
authors: N
authors: N
authors: Hiroi
authors: G
authors: G
authors: Eisenhofer
authors: S M
authors: SM
authors: McCann
authors: G P
authors: GP
authors: Chrousos
authors: S
authors: S
authors: Roffler-Tarlov
mesh_terms: D000311:Adrenal Glands / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D000818:Animals; D000943:Antigens, Differentiation / Q000032:analysis; D002395:Catecholamines / Q000097:blood / Q000378:metabolism*; D019439:Chromaffin Cells / Q000378:metabolism*; D002864:Chromogranins / Q000235:genetics; D003345:Corticosterone / Q000378:metabolism; D004744:Enkephalin, Methionine / Q000032:analysis; D004745:Enkephalins / Q000235:genetics; D005260:Female; D017353:Gene Deletion; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009478:Neuropeptide Y / Q000032:analysis / Q000235:genetics; D010625:Phenylethanolamine N-Methyltransferase / Q000235:genetics; D011498:Protein Precursors / Q000235:genetics; D012333:RNA, Messenger; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics / Q000502:physiology*; D043222:Ubiquitin Thiolesterase
chemical_list: D000943:Antigens, Differentiation; D002395:Catecholamines; D002864:Chromogranins; D004745:Enkephalins; D009478:Neuropeptide Y; D011498:Protein Precursors; D012333:RNA, Messenger; C029992:proenkephalin; D004744:Enkephalin, Methionine; D014446:Tyrosine 3-Monooxygenase; D010625:Phenylethanolamine N-Methyltransferase; D043222:Ubiquitin Thiolesterase; D003345:Corticosterone