Fgf8 is required for outgrowth and patterning of the limbs.
The expression pattern and activity of fibroblast growth factor-8 (FGF8) in experimental assays indicate that it has important roles in limb development, but early embryonic lethality resulting from mutation of Fgf8 in the germ line of mice has prevented direct assessment of these roles. Here we report that conditional disruption of Fgf8 in the forelimb of developing mice bypasses embryonic lethality and reveals a requirement for Fgf8 in the formation of the stylopod, anterior zeugopod and autopod. Lack of Fgf8 in the apical ectodermal ridge (AER) alters expression of other Fgf genes, Shh and Bmp2.
Moon
A M
AM
Department of Pediatrics and Human Molecular Biology and Genetics, University of Utah School of Medicine, Salt Lake City, Utah, USA.
Capecchi
M R
MR
D000818:Animals; D019521:Body Patterning / Q000235:genetics; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000235:genetics / Q000502:physiology; D004475:Ectoderm / Q000378:metabolism; D005121:Extremities / Q000196:embryology*; D051524:Fibroblast Growth Factor 8; D005346:Fibroblast Growth Factors / Q000235:genetics* / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D018390:Gene Targeting; D053823:Hedgehog Proteins; D017403:In Situ Hybridization; D017880:Limb Deformities, Congenital / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout; D011506:Proteins / Q000235:genetics / Q000502:physiology; D015534:Trans-Activators; D016212:Transforming Growth Factor beta
C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; C496457:Fgf8 protein, mouse; D053823:Hedgehog Proteins; D011506:Proteins; D015534:Trans-Activators; D016212:Transforming Growth Factor beta; D051524:Fibroblast Growth Factor 8; D005346:Fibroblast Growth Factors