The spatial and temporal expression pattern of Nesp and its antisense Nespas, in mid-gestation mouse embryos.
We describe the spatiotemporal expression pattern of Nesp, and its antisense transcript, Nespas. We found non-complementary expression of these two oppositely imprinted transcripts during mouse embryogenesis, in a number of forming embryonic structures. Nesp expression was primarily seen in the somites and vasculature, whereas Nespas was mainly detected in the progress zone, mesenchyme and ectoderm of the limb, and the neural tube.
Ball
S T
ST
Mammalian Genetics Unit, Medical Research Council, Harwell, Didcot, Oxfordshire, OX11 0RD, UK.
Williamson
C M
CM
Hayes
C
C
Hacker
T
T
Peters
J
J
D000818:Animals; D001808:Blood Vessels / Q000196:embryology; D002864:Chromogranins; D004475:Ectoderm / Q000378:metabolism; D004622:Embryo, Mammalian / Q000378:metabolism*; D005121:Extremities / Q000196:embryology; D019205:GTP-Binding Protein alpha Subunits, Gs; D015870:Gene Expression; D018392:Genomic Imprinting; D008648:Mesoderm / Q000378:metabolism; D051379:Mice; D008809:Mice, Inbred C3H; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics*; D009432:Neural Crest / Q000196:embryology; D016376:Oligonucleotides, Antisense / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D019170:Somites / Q000378:metabolism; D013997:Time Factors
D002864:Chromogranins; D009419:Nerve Tissue Proteins; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; C498642:Gnas protein, mouse; D019205:GTP-Binding Protein alpha Subunits, Gs