title: Advances in the characterization of supported lipid films with the atomic force microscope.
abstract: During the past decade, the atomic force microscope (AFM) has become a key technique in biochemistry and biophysics to characterize supported lipid films, as testified by the continuous growth in the number of papers published in the field. The unique capabilities of AFM are: (i) capacity to probe, in real time and in aqueous environment, the surface structure of lipid films; (ii) ability to directly measure physical properties at high spatial resolution; (iii) possibility to modify the film structure and biophysical processes in a controlled way. Such experiments, published up to June 2000, are the focus of the present review. First, we provide a general introduction on the preparation and characterization of supported lipid films as well as on the principles of AFM. The section 'Structural properties' focuses on the various applications of AFM for characterizing the structure of supported lipid films: visualization of molecular structure, formation of structural defects, effect of external agents, formation of supported films, organization of phase-separated films (coexistence region, mixed films) and, finally, the use of supported lipid bilayers for anchoring biomolecules such as DNA, enzymes and crystalline protein arrays. The section 'Physical properties' introduces the principles of force measurements by AFM, interpretation of these measurements and their recent application to supported lipid films and related structures. Finally, we highlight the major achievements brought by the technique and some of the current limitations.
authors: Unité de chimie des interfaces, Université catholique de Louvain, Belgium. dufrene@cifa.ucl.ac.be
authors: Y F
authors: YF
authors: Dufrêne
authors: G U
authors: GU
authors: Lee
mesh_terms: D000388:Air; D003461:Crystallography; D008051:Lipid Bilayers / Q000737:chemistry*; D008074:Lipoproteins / Q000737:chemistry; D018625:Microscopy, Atomic Force; D008968:Molecular Conformation; D015394:Molecular Structure; D010743:Phospholipids / Q000737:chemistry; D012997:Solvents; D013499:Surface Properties; D014867:Water
chemical_list: D008051:Lipid Bilayers; D008074:Lipoproteins; D010743:Phospholipids; D012997:Solvents; D014867:Water