The extraneuronal monoamine transporter Slc22a3/Orct3 co-localizes with the Maoa metabolizing enzyme in mouse placenta.
Monoamine clearance is a combined function of uptake mechanisms in the plasma membrane with intracellular metabolizing enzymes. Two different uptake mechanisms have been described. Uptake(1) is located in presynaptic neurones, whereas uptake(2) is extraneuronal. Recently, the Slc22a3/Orct3 gene was identified as the extraneuronal monoamine transporter. In mouse embryonic development Orct3 expression is restricted to the placenta, which is also a site of expression of neuronal transporters. We have used RNA blots and in situ hybridization to examine the expression of Orct3 and other members of the monoamine uptake and metabolizing pathways in mouse placenta. The results show that Orct3 expression overlaps that of the monoamine metabolizing enzyme Maoa in the labyrinth layer of the placenta with an expression pattern distinct from that of the neuronal transporters Slc6a2/Net and Slc6a4/Sert.
Verhaagh
S
S
Department of Molecular Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands.
Barlow
D P
DP
Zwart
R
R
D000588:Amines / Q000493:pharmacokinetics; D000818:Animals; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000378:metabolism; D017403:In Situ Hybridization; D008562:Membrane Glycoproteins / Q000378:metabolism; D026901:Membrane Transport Proteins; D051379:Mice; D008969:Molecular Sequence Data; D008995:Monoamine Oxidase / Q000378:metabolism*; D009419:Nerve Tissue Proteins; D009474:Neurons / Q000378:metabolism; D027701:Organic Cation Transport Proteins; D010920:Placenta / Q000378:metabolism*; D012313:RNA / Q000378:metabolism; D050486:Serotonin Plasma Membrane Transport Proteins; D013997:Time Factors; D000099254:Organic Cation Transporter 3
D000588:Amines; D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D008995:Monoamine Oxidase; D009419:Nerve Tissue Proteins; D027701:Organic Cation Transport Proteins; D012313:RNA; D050486:Serotonin Plasma Membrane Transport Proteins; D000099254:Organic Cation Transporter 3; C493231:Slc6a4 protein, mouse