Glutathione attenuates coronary constriction to acetylcholine in patients with coronary spastic angina.
This study examined the effect of reduced glutathione (GSH), an important antioxidant that restores intracellular redox imbalance and prevents inactivation of endothelial-derived nitric oxide, on the abnormal vasomotor reactivity in spastic coronary arteries. The responses of epicardial diameter of the left coronary arteries to intracoronary infusion of acetylcholine (ACh; 50 microg/min) were measured by quantitative coronary angiography before and during combined intracoronary infusion of GSH (50 mg/min for 6 min) or saline as a placebo in 24 patients with coronary spastic angina and in 28 control patients. All of the spastic coronary arteries showed constrictor response to ACh, whereas the control coronary arteries as a whole showed only minimal diameter changes to ACh. GSH infusion suppressed constrictor response of epicardial diameter to ACh in patients with coronary spastic angina, whereas it had no significant effect in control subjects. Saline infusion did not have any effects. The results indicate that GSH attenuated the constrictor response to ACh in epicardial coronary arteries of patients with coronary spastic angina. GSH may have an important role in the regulation of coronary vasomotor function in patients with coronary spastic angina.
Kugiyama
K
K
Department of Cardiovascular Medicine, Kumamoto University School of Medicine, Kumamoto, Japan 860-8556. kiyo@gpo.kumamoto-u.ac.jp
Miyao
Y
Y
Sakamoto
T
T
Kawano
H
H
Soejima
H
H
Miyamoto
S
S
Yoshimura
M
M
Ogawa
H
H
Sugiyama
S
S
Yasue
H
H
D000109:Acetylcholine; D000328:Adult; D000368:Aged; D000788:Angina Pectoris, Variant / Q000503:physiopathology*; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D001794:Blood Pressure / Q000187:drug effects; D017023:Coronary Angiography; D003326:Coronary Circulation / Q000187:drug effects*; D004562:Electrocardiography; D005260:Female; D005978:Glutathione / Q000378:metabolism / Q000494:pharmacology*; D006339:Heart Rate / Q000187:drug effects; D006801:Humans; D008297:Male; D008875:Middle Aged; D009249:NADP / Q000378:metabolism; D005996:Nitroglycerin / Q000494:pharmacology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014662:Vasoconstrictor Agents; D014665:Vasodilator Agents / Q000494:pharmacology
D000975:Antioxidants; D017392:Thiobarbituric Acid Reactive Substances; D014662:Vasoconstrictor Agents; D014665:Vasodilator Agents; D009249:NADP; D005996:Nitroglycerin; D005978:Glutathione; D000109:Acetylcholine