Chromosome arm-specific multicolor FISH.
Several systems for 24-color fluorescence in situ hybridization (FISH) have been developed and applied to karyotyping and detection of chromosomal abnormalities. We have developed a 42-color multicolor FISH (mFISH) technique (armFISH), which permits the detection of chromosomal aberrations at the resolution of chromosome arms. The armFISH uses a commercially available mFISH reagent kit (24XCyte, MetaSystems GmbH) supplemented with a set of differentially labeled chromosome arm-specific painting probes (arm-kit, comprising either p- or q-arms of all human chromosomes, except the p-arm of the acrocentric- and Y chromosomes). The mFISH-probe cocktail and the arm-kit are combined and hybridized together to metaphase chromosomes. The armFISH is analyzed in two steps; first, the conventional mFISH image analysis is performed, followed by the arm-kit analysis to reveal the chromosome arms involved. The examples demonstrate the utility of armFISH in defining chromosomal rearrangements of human cancers.
Karhu
R
R
Laboratory of Cancer Genetics, University of Tampere and Tampere University Hospital, Tampere, Finland. ritva.karhu@uta.fi
Ahlstedt-Soini
M
M
Bittner
M
M
Meltzer
P
P
Trent
J M
JM
Isola
J J
JJ
D002232:Carbocyanines; D002869:Chromosome Aberrations / Q000235:genetics; D020223:Chromosome Painting / Q000379:methods*; D005456:Fluorescent Dyes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
D002232:Carbocyanines; D005456:Fluorescent Dyes; D011933:Reagent Kits, Diagnostic; C085321:cyanine dye 5