Correlation between plasma steroid hormones and vitellogenin profiles and lunar periodicity in the female golden rabbitfish, Siganus guttatus (Bloch).
Characteristics of the lunar reproductive cycle in the golden rabbitfish, Siganus guttatus, were determined by histological observations of ovarian development, and immunological measurements of plasma steroid hormones, estradiol-17beta (E2), testosterone (T), 17alpha,20beta-dihydroxy-4-pregnen-3-one (DHP) and 17alpha,20beta,21-trihydroxy-4-pregnen-3-one (20beta-S), and vitellogenin (VTG). Ovarian and plasma samples were collected every week according to the lunar phases from May to July. Weekly change of gonadosomatic index (GSI) showed two peaks at the first lunar quarter in June and July. Yolky oocytes were also observed around this time. Histological observations revealed that the vitellogenic oocytes appeared again 1 week after spawning and developed synchronously. These results suggest that this species is a multiple spawner and the oocyte development is in a group-synchronous manner. Plasma steroid hormones (E2, T, DHP and 20beta-S) and VTG levels changed in parallel with changes in GSI. The peak of plasma VTG level occurred prior to spawning. These cyclic changes of plasma steroid hormones and VTG support the hypothesis that lunar periodicity is the major factor in stimulating reproductive activity of S. guttatus.
Rahman
M D
MD
Sesoko Station, Tropical Biosphere Research Center, University of the Ryukyus, Okinawa, Japan.
Takemura
A
A
Takano
K
K
D000818:Animals; D003350:Cortodoxone / Q000031:analogs & derivatives* / Q000097:blood; D004958:Estradiol / Q000097:blood; D005260:Female; D006908:Hydroxyprogesterones / Q000097:blood; D016081:Moon; D009903:Optical Illusions; D010053:Ovary / Q000473:pathology / Q000502:physiology; D010473:Perciformes / Q000097:blood* / Q000502:physiology*; D012621:Seasons; D013256:Steroids / Q000097:blood*; D013739:Testosterone / Q000097:blood; D013997:Time Factors; D014819:Vitellogenins / Q000097:blood
D006908:Hydroxyprogesterones; D013256:Steroids; D014819:Vitellogenins; C021012:17 alpha,20 beta-dihydroxypregn-4-en-3-one; D013739:Testosterone; D004958:Estradiol; C051954:17,20,21-trihydroxy-4-pregnen-3-one; D003350:Cortodoxone