title: Functional analysis of regulatory elements controlling the expression of the ecdysone-regulated Drosophila ng-1 gene.
abstract: The steroid hormone ecdysone controls multiple aspects of insect development, including larval moults and metamorphosis, and can induce specific genetic responses in different tissues. The definition of the molecular mechanisms able to mediate this tissue-specific responsiveness may greatly contribute to understanding how such an accurate genetic response is achieved. In this work we have identified, by transgenic analysis, the regulatory elements directing the expression of ng-1, an ecdysone-regulated Drosophila gene showing a highly specific developmental expression profile. Our results show that an ecdysone-responsive element located within the ng-1 coding region is necessary for high-level gene expression, whereas the gene's spatial and temporal expression profile is fully controlled by a distinct upstream regulatory region. This region binds a set of transcriptional factors, including the FKH regulatory protein, which can potentially modulate the ecdysone genetic regulated response.
authors: Dipartimento di Genetica, Biologia Generale e Molecolare, Università di Napoli, via Mezzocannone 8, I-80134 Napoli, Italy.
authors: S
authors: S
authors: Crispi
authors: E
authors: E
authors: Giordano
authors: P P
authors: PP
authors: D'Avino
authors: I
authors: I
authors: Peluso
authors: M
authors: M
authors: Furia
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D015152:Blotting, Northern; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000196:embryology*; D004440:Ecdysone / Q000378:metabolism*; D051858:Forkhead Transcription Factors; D018507:Gene Expression Regulation, Developmental; D007763:Lac Operon; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D012471:Salivary Proteins and Peptides / Q000235:genetics* / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D013997:Time Factors; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D051858:Forkhead Transcription Factors; D009687:Nuclear Proteins; D012471:Salivary Proteins and Peptides; D014157:Transcription Factors; C081602:ng1 protein, Drosophila; D004440:Ecdysone; D012313:RNA; D001616:beta-Galactosidase