Determination of lovastatin in human plasma using reverse-phase high-performance liquid chromatography with UV detection.
The authors have developed a simple, rapid HPLC assay with ultraviolet (UV) detection for the analytical determination of lovastatin and its acid in human plasma for a concentration range of 100-5,000 ng/mL. Sample clean-up involved the use of C10 solid-phase extraction cartridges. Our limit of quantitation was 100 ng/mL. Standard curves were linear from 100 to 5,000 ng/mL, with a correlation coefficient (r2) of 0.999 +/- 0.0002. Stored samples were stable at -70 degrees C for up to 4 months prior to reversed-phase HPLC analysis. This assay was able to measure steady-state lovastatin concentration (Css) at the initial dose level in a phase I trial of lovastatin as a modulator of apoptosis.
Ye
L Y
LY
Department of Experimental Therapeutics, Faculty of Pharmacy, University of Toronto, Ontario, Canada.
Firby
P S
PS
Moore
M J
MJ
D000924:Anticholesteremic Agents / Q000097:blood*; D002138:Calibration; D002264:Carboxylic Acids / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D016903:Drug Monitoring / Q000379:methods; D006801:Humans; D006868:Hydrolysis; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000097:blood*; D008148:Lovastatin / Q000097:blood*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
D000924:Anticholesteremic Agents; D002264:Carboxylic Acids; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D008148:Lovastatin