Characterization of the gene encoding a histidine and aspartic acid-rich protein from Pneumocystis carinii.
A cDNA clone derived from Pneumocystis carinii contained an unusual sequence (GTGATG)2(ATGGTG)4(ATG)4 and many GAT repeats. It was found to encode a histidine and aspartic acid-rich protein (HARP). The complete cDNA contained an 888-bp open reading frame encoding a putative protein of 32.6 kDa. The deduced HARP protein contained 39 aspartic acid and 22 histidine residues. The genomic copy of the HARP gene (1203 bp in length) was found to contain 3 small introns of 46, 44, and 38 bp, respectively. HARP was predicted by computer programs to be a plasma membrane protein with nickel-binding activity.
Lee
C H
CH
Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis 46202, USA. chlee@iupui.edu
Lasbury
M E
ME
Paulsrud
J R
JR
Bauer
N L
NL
Brady
S L
SL
Weinberg
G A
GA
Durant
P J
PJ
Bartlett
M S
MS
Smith
J W
JW
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001224:Aspartic Acid / Q000032:analysis; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D005800:Genes, Fungal; D006639:Histidine / Q000032:analysis; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009532:Nickel / Q000378:metabolism; D016366:Open Reading Frames; D011010:Pneumocystis / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017434:Protein Structure, Tertiary; D012091:Repetitive Sequences, Nucleic Acid; D017386:Sequence Homology, Amino Acid; D012984:Software
D018076:DNA, Complementary; D004271:DNA, Fungal; D005656:Fungal Proteins; D008565:Membrane Proteins; C420082:histidine-aspartic acid-rich protein, Pneumocystis carinii; D001224:Aspartic Acid; D006639:Histidine; D009532:Nickel