Variant form of diffuse corporal gastritis in NHE2 knockout mice.
Mice lacking the NHE2 Na+/H+ gene develop gastritis of the glandular mucosa as early as the tenth day of life, achieving maximal intensity of inflammation from 17 to 19 days after birth and maximal atrophy at one year. We assessed the effects of this process in such mice to 16 months of age. The stomach of NHE2 null mutants was examined at 10, 17 to 20, 24 to 35 and 49 to 70 days, and at 12 to 16 months. The NHE2 wild-type (+/+) and NHE2 heterozygous (+/-) mice were compared with the NHE2 homozygous mutant mice (-/-). The stomach of the mutant mice at all ages was characterized by a substantially reduced number of parietal cells. The 10-day-old mouse stomach had a transmural infiltrate of primarily neutrophils. With increasing age, neutrophils were replaced by lymphocytes and plasma cells in the glandular mucosa of the mutant mice. Young adult 49- to 70-day-old mice had surface cell hyperplasia and expansion of the replicating cell population. Hyperplasia of enterochromaffin-like cells and antral gastrin cells accompanied profound fundic gland and surface cell hyperplasia, and became progressively more severe with increasing age of the NHE2-/- mice. Neoplasms were not found in the mutant or control mice. This gastritis differs from that of autoimmune gastritis in that it is transmural, begins in infancy, and is associated with a predominantly neutrophilic infiltrate in its early stages. Some of the histologic changes in the adult mice can be explained on the basis of prolonged achlorhydria. This mouse may be a suitable model for prolonged effects of achlorhydria.
Boivin
G P
GP
Department of Pathology and Laboratory Medicine, University of Cincinnati School of Medicine, Ohio 45267-0529, USA.
Schultheis
P J
PJ
Shull
G E
GE
Stemmermann
G N
GN
D000367:Age Factors; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D005753:Gastric Mucosa / Q000473:pathology*; D005757:Gastritis, Atrophic / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D007150:Immunohistochemistry / Q000662:veterinary; D008297:Male; D051379:Mice; D018345:Mice, Knockout / Q000502:physiology*; D012376:Rodent Diseases / Q000235:genetics / Q000473:pathology*; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics / Q000502:physiology*; D013270:Stomach / Q000473:pathology*
C513111:Slc9a2 protein, mouse; D017923:Sodium-Hydrogen Exchangers