Gene transfer into the central nervous system using herpes simplex virus-1 vectors.
Manipulation of gene expression in developing or in mature central nervous systems (CNS) holds a promise for the resolution of many compelling neurobiological questions, including the feasibility of gene therapy to treat diseases of the brain. In this context, a number of viral vectors have been used in recent years to introduce and express genes into the CNS. This article discusses a gene transfer system based on the Herpes Simplex Virus-1 (HSV-1). We describe here the use of non-replicating, non-toxic HSV-1 vector, 8117/43, in a series of studies carried in our joint program. This vector proves further the utility of HSV-1 as a delivery vehicle to a number of distinct sites within the CNS.
Tabbaa
S
S
Department of Anatomy and Cell Biology, State University of New York, Buffalo, USA.
Goulah
C
C
Tran
R K
RK
Lis
A
A
Korody
R
R
Stachowski
B
B
Horowitz
J M
JM
Torres
G
G
Stachowiak
E K
EK
Bloom
D C
DC
Stachowiak
M K
MK
D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors; D018259:Herpesvirus 1, Human; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D013238:Stereotaxic Techniques; D001616:beta-Galactosidase / Q000032:analysis / Q000235:genetics*
D001616:beta-Galactosidase