title: Induction of the chemokine stromal-derived factor-1 following DNA damage improves human stem cell function.
abstract: The chemokine stromal-derived factor-1 (SDF-1) controls many aspects of stem cell function. Details of its regulation and sites of production are currently unknown. We report that in the bone marrow, SDF-1 is produced mainly by immature osteoblasts and endothelial cells. Conditioning with DNA-damaging agents (ionizing irradiation, cyclophosphamide, and 5-fluorouracil) caused an increase in SDF-1 expression and in CXCR4-dependent homing and repopulation by human stem cells transplanted into NOD/SCID mice. Our findings suggest that immature osteoblasts and endothelial cells control stem cell homing, retention, and repopulation by secreting SDF-1, which also participates in host defense responses to DNA damage.
authors: Department of Immunology, The Weizmann Institute of Science, Rehovot, Israel.
authors: T
authors: T
authors: Ponomaryov
authors: A
authors: A
authors: Peled
authors: I
authors: I
authors: Petit
authors: R S
authors: RS
authors: Taichman
authors: L
authors: L
authors: Habler
authors: J
authors: J
authors: Sandbank
authors: F
authors: F
authors: Arenzana-Seisdedos
authors: A
authors: A
authors: Magerus
authors: A
authors: A
authors: Caruz
authors: N
authors: N
authors: Fujii
authors: A
authors: A
authors: Nagler
authors: M
authors: M
authors: Lahav
authors: M
authors: M
authors: Szyper-Kravitz
authors: D
authors: D
authors: Zipori
authors: T
authors: T
authors: Lapidot
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D002460:Cell Line; D002478:Cells, Cultured; D054377:Chemokine CXCL12; D019743:Chemokines, CXC / Q000235:genetics*; D003520:Cyclophosphamide / Q000494:pharmacology; D004249:DNA Damage; D004307:Dose-Response Relationship, Radiation; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D005434:Flow Cytometry; D005472:Fluorouracil / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016513:Mice, SCID; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D019743:Chemokines, CXC; C515256:Cxcl12 protein, mouse; D012333:RNA, Messenger; D003520:Cyclophosphamide; D005472:Fluorouracil