Detection of plasmalogen from plasma low density lipoprotein and high density lipoprotein in carp, Cyprinus carpio, and rainbow trout, Oncorhynchus mykiss.
The study revealed the presence of plasmalogens in the low density lipoprotein (LDL) and high density lipoprotein (HDL) of the fish. The composition of the plasmalogen in the carp plasma LDL phospholipids was 0.94 and 0.23% in the HDL; the LDL phospholipids in the rainbow trout were 0.44% and the HDL was 0.18%. Aldehydes from the plasmalogen were derivatized with dansylhydrazides and separated by high performance liquid chromatography (HPLC). Their presence was detected using a fluorescence detector. Hexadecanal (C16: 0), octadecanal (C18: 0) and octadecenal (C18: 1) were determined to be the major components in the carp and rainbow trout.
Yamaguchi
T
T
Laboratory of Marine Biochemistry, Graduate School of Agricultural Science, Tohoku University, 1-1, Tsutsumidori-Amamiya, Aoba-ku, 981-8555, Sendai, Japan. ymg@bios.tohoku.ac.jp
Miyamoto
K
K
Yagi
S
S
Horigane
A
A
Sato
M
M
Takeuchi
M
M
D000818:Animals; D002347:Carps / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D008075:Lipoproteins, HDL / Q000097:blood / Q000737:chemistry*; D008077:Lipoproteins, LDL / Q000097:blood / Q000737:chemistry*; D013058:Mass Spectrometry; D017686:Oncorhynchus mykiss / Q000097:blood*; D010955:Plasmalogens / Q000032:analysis* / Q000097:blood; D013050:Spectrometry, Fluorescence
D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D010955:Plasmalogens