Identification of STKA-dependent genes in Dictyostelium discoideum.
During culmination of Dictyostelium aggregates, prespore and prestalk cells undergo terminal differentiation to form spores and a cellular stalk. Disruption of the cell-fate gene stkA leads to a phenotype in which all the cells destined to become spores end up as stalk cells. 'Stalky' mutants express normal levels of prespore cell transcripts but fail to produce the culmination-stage spore transcript spiA. The stkA gene encodes a putative GATA-type transcription factor (STKA). In order to identify possible downstream targets of STKA we used the technique of mRNA differential display and isolated four cDNA fragments that hybridise to mRNAs present during the later stages of development. All four gene tags were cloned and sequenced. mRNAs represented by these four sequence tags do not accumulate during culmination of 'stalky' cells and therefore must be specific to the spore pathway. By screening a cDNA library, longer cDNAs for all four were cloned and sequenced. Three of these contained complete protein-coding regions while only a partial cDNA was recovered for the fourth. One of the corresponding proteins has significant homology to a surface zinc metalloproteinase (GP63) of the protozoan parasite Leishmania, while another is closely related to a human pre-RNA binding protein (hnRNP R).
Loughran
G
G
Dept. of Biochemistry, University of Oxford, United Kingdom.
Pinter
K
K
Newell
P C
PC
Gross
J D
JD
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D003001:Cloning, Molecular; D004023:Dictyostelium / Q000166:cytology / Q000235:genetics / Q000502:physiology*; D005786:Gene Expression Regulation; D015723:Gene Library; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D006801:Humans; D008666:Metalloendopeptidases / Q000235:genetics; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism*; D015800:Protozoan Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics; D012261:Ribonucleoproteins / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013170:Spores / Q000502:physiology; D014158:Transcription, Genetic
C439930:HNRNPR protein, human; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D009687:Nuclear Proteins; D015800:Protozoan Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; C102198:stkA protein, Dictyostelium discoideum; D008666:Metalloendopeptidases; C055146:glycoprotein gp63, Leishmania