RISSC: a novel database for ribosomal 16S-23S RNA genes spacer regions.
A novel database, under the acronym RISSC (Ribosomal Intergenic Spacer Sequence Collection), has been created. It compiles more than 1600 entries of edited DNA sequence data from the 16S-23S ribosomal spacers present in most prokaryotes and organelles (e.g. mitochondria and chloroplasts) and is accessible through the Internet (http://ulises.umh.es/RISSC), where systematic searches for specific words can be conducted, as well as BLAST-type sequence searches. Additionally, a characteristic feature of this region, the presence/absence and nature of tRNA genes within the spacer, is included in all the entries, even when not previously indicated in the original database. All these combined features could provide a useful documentation tool for studies on evolution, identification, typing and strain characterization, among others.
García-Martínez
J
J
División de Microbiología, Campus de San Juan, Universidad Miguel Hernández, Elche, Alicante, Spain.
Bescós
I
I
Rodríguez-Sala
J J
JJ
Rodríguez-Valera
F
F
D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D016208:Databases, Factual; D020407:Internet; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012338:RNA, Ribosomal, 23S / Q000235:genetics*; D016415:Sequence Alignment
D021903:DNA, Ribosomal Spacer; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S