Absorption by rats of tocopherols present in edible vegetable oils.
The absorption of tocopherols (alpha, gamma, and delta) and fatty acids from rapeseed (RO), soybean (SOO), and sunflower (SUO) oil, both from the natural oils and from the oils following moderate heating (180 degrees C for 15 min), was measured in lymph-cannulated rats. Oils were administered as emulsions through a gastrostomy tube, and lymph samples were collected for 24 h. The composition of tocopherols in oils and lymph fractions was measured by high-performance liquid chromatography, and fatty acids were measured by gas-liquid chromatography. The highest accumulated transport of alpha-tocopherol was observed after SUO administration, the lowest after SOO, with RO in between, corresponding to their relative contents (41.6 +/- 8.8, 32.7 +/- 5.0, and 24.9 +/- 4.3 microg at 24 h after administration of SUO, RO, and SOO, respectively). The calculated recoveries (in %) 24 h after oil administration were 21.4 +/- 4.5, 45.7 +/- 7.0, and 78.8 +/- 13.5 for SUO, RO, and SOO, respectively, suggesting that the absorption efficiency decreased when the alpha-tocopherol concentration increased. The recovery of alpha-tocopherol was higher than the recoveries of gamma- and delta-tocopherol, indicating that the different tocopherols were not absorbed to the same extent or with similar rates. No differences between unheated and heated oils were observed in the absorption of tocopherols, whereas heating led to lower absorption of fatty acids, thus showing no direct association between absorption of tocopherols and fatty acids.
Porsgaard
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T
Department of Biochemistry and Nutrition and Center for Food Research, Technical University of Denmark, Lyngby. trine@mimer.be.dtu.dk
Høy
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CE
D000042:Absorption; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004032:Diet; D005227:Fatty Acids / Q000378:metabolism; D005229:Fatty Acids, Monounsaturated; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D010938:Plant Oils / Q000493:pharmacokinetics*; D000074262:Rapeseed Oil; D051381:Rats; D017208:Rats, Wistar; D013024:Soybean Oil / Q000493:pharmacokinetics; D000074242:Sunflower Oil; D013997:Time Factors; D014810:Vitamin E / Q000493:pharmacokinetics*
D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D010938:Plant Oils; D000074262:Rapeseed Oil; D000074242:Sunflower Oil; D014810:Vitamin E; D013024:Soybean Oil