Evidence of nucleophilic addition to chemiluminescent N-sulfonylacridinium-9-carboxamides from electrospray ionization mass spectrometry.
Addition of nucleophiles to the chemiluminescent acridinium-9-carboxamide 1 resulted in the formation of the acridan adduct 7. The relative ratio of 1/7 present in solution could be determined by electrospray ionization mass spectrometry (ESI-MS) for a number of nucleophiles that are commonly employed during the use of 1 as a label in medical diagnostic assays.
Adamczyk
M
M
Abbott Laboratories, Department of Chemistry, Abbott Park, Illinois 60064-6106, USA. maciej.adamczyk@abbott.com
Gebler
J C
JC
Mattingly
P G
PG
Wu
J
J
D000166:Acridines / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D011506:Proteins / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
D000166:Acridines; D007202:Indicators and Reagents; D011506:Proteins; D006861:Hydrogen Peroxide