Purification of tyrosine-phosphorylated proteins by immunoaffinity chromatography and direct cloning of their cDNAs from bacterial expression libraries.
Di Fiore
P P
PP
Department of Experimental Oncology, European Institute of Oncology, Milan, Italy.
Fazioli
F
F
D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D001419:Bacteria; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D002846:Chromatography, Affinity / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003001:Cloning, Molecular / Q000379:methods; D018076:DNA, Complementary; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism*; D015723:Gene Library; D006801:Humans; D007202:Indicators and Reagents; D010750:Phosphoproteins / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D019000:Phosphotyrosine / Q000378:metabolism*; D011817:Rabbits; D020794:Receptor Protein-Tyrosine Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism
D000906:Antibodies; D000911:Antibodies, Monoclonal; D018076:DNA, Complementary; D007202:Indicators and Reagents; D010750:Phosphoproteins; D011994:Recombinant Proteins; D019000:Phosphotyrosine; D066246:ErbB Receptors; D020794:Receptor Protein-Tyrosine Kinases