Modes of annexin-membrane interactions analyzed by employing chimeric annexin proteins.
Annexin II is a member of the annexin family of Ca(2+)- and phospholipid-binding proteins which is particularly enriched on early endosomal membranes and has been implicated in participating in endocytic events. In contrast to other endosomal annexins the association of annexin II with its target membrane can occur in the absence of Ca(2+) in a manner depending on the unique N-terminal domain of the protein. However, endosome binding of annexin II does not require formation of a protein complex with the intracellular ligand S100A10 (p11) as an annexin II mutant protein (PM AnxII) incapable of interacting with p11 is still present on endosomal membranes. Fusion of the N-terminal sequence of this PM AnxII (residues 1-27) to the conserved protein core of annexin I transfers the capability of Ca(2+)-independent membrane binding to the otherwise Ca(2+)-sensitive annexin I. These results underscore the importance of the N-terminal sequence of annexin II for the Ca(2+)-independent endosome association and argue for a direct interaction of this sequence with an endosomal membrane receptor.
König
J
J
Institute for Medical Biochemistry, Centre for Molecular Biology of Inflammation, University of Münster, von-Esmarch-Strasse 56, D-48149, Münster, Germany.
Gerke
V
V
D000595:Amino Acid Sequence; D000818:Animals; D017306:Annexin A2 / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D017302:Annexins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D002678:Chimera; D004072:Digitonin; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D013347:Subcellular Fractions / Q000378:metabolism
D017306:Annexin A2; D017302:Annexins; D004072:Digitonin; D002118:Calcium