title: Stabilization of O-pyromellitylgramicidin channels in bilayer lipid membranes through electrostatic interaction with polylysines of different chain lengths.
abstract: Functioning of membrane proteins, in particular ionic channels, can be modulated by alteration of their arrangement in membranes. We addressed this issue by studying the effect of different chain length polylysines on the kinetics of ionic channels formed in a bilayer lipid membrane (BLM) by O-pyromellitylgramicidin carrying three negative charges at the C-terminus. The method of sensitized photoinactivation was applied to the analysis of the channel association-dissociation kinetics (characterized by the exponential factor of the curve describing the time course of the flash-induced decrease in the transmembrane current, tau). Addition of polylysine to the bathing solutions of BLM led to the deceleration of the photoinactivation kinetics, i.e. to the increase in tau. It was shown here that for a series of polylysines differing in their chain lengths, the value of tau grew as their concentration increased above a threshold level until at a certain concentration of each polylysine tau reached maximum. At higher polylysine concentrations tau began to decrease and finally became close to the control level observed in the absence of polylysine. With lengthening of the polylysine chain the maximum value of tau increased, the concentration dependence became steeper, and the threshold concentration decreased. The increase in the ionic strength of the medium shifted the concentration dependence of tau to higher polylysine concentrations and decreased the maximum value of tau. It was concluded that the increase in tau was caused by the formation of domains of O-pyromellitylgramicidin molecules induced by binding of polylysines. This can be related to functional aspects of polycation-induced sequestering of negatively charged transmembrane peptides in neutral membranes.
authors: A.N Belozersky Institute of Physio-Chemical Biology, Moscow State University, Russia.
authors: A V
authors: AV
authors: Krylov
authors: E A
authors: EA
authors: Kotova
authors: A A
authors: AA
authors: Yaroslavov
authors: Y N
authors: YN
authors: Antonenko
mesh_terms: D019281:Dimerization; D004305:Dose-Response Relationship, Drug; D006096:Gramicidin / Q000031:analogs & derivatives* / Q000737:chemistry; D007473:Ion Channels / Q000737:chemistry*; D007700:Kinetics; D008027:Light; D008051:Lipid Bilayers / Q000737:chemistry*; D008956:Models, Chemical; D017319:Photosensitizing Agents / Q000737:chemistry; D011107:Polylysine / Q000737:chemistry* / Q000494:pharmacology; D055672:Static Electricity
chemical_list: D007473:Ion Channels; D008051:Lipid Bilayers; D017319:Photosensitizing Agents; D006096:Gramicidin; D011107:Polylysine