Detection of diarrhetic shellfish poisoning toxins from tropical shellfish using liquid chromatography-selected reaction monitoring mass spectrometry.
A negative mode liquid chromatography-selected reaction monitoring mass spectrometry (LC-SRM MS) method was developed to detect low concentrations of the diarrhetic shellfish poisoning (DSP) toxins okadaic acid and dinophysistoxin-1 (DTX-1). Detection relies upon monitoring the transition of negative precursor ions [M - H]- to a common fragment ion of m/z 255. Our limit of detection for okadaic acid with this method is 0.5 pg on column. LC-SRM MS has allowed us to detect persistent, low concentrations of DSP toxins from Singapore shellfish.
Holmes
M J
MJ
Department of Biological Sciences and National University of Singapore, Singapore. dbshmj@nus.edu.sg
Teo
S L
SL
Khoo
H W
HW
D000818:Animals; D049872:Bivalvia / Q000737:chemistry; D002845:Chromatography / Q000379:methods*; D019319:Okadaic Acid / Q000032:analysis* / Q000031:analogs & derivatives; D011714:Pyrans / Q000032:analysis*; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D011714:Pyrans; D019319:Okadaic Acid; C051904:dinophysistoxin 1