Voltammetric and spectroscopic studies on methyl green and cationic lipid bound to calf thymus DNA.
DNA interaction with cationic lipids promises to be a versatile and effective synthetic transfection agent. This paper presents the study on binding of a simple artificial cationic lipid, cetyltrimethylammonium bromide (CTAB), to calf thymus DNA (CT DNA) prior to the condensation process, taking methyl green (MG) as a probe. The results show that the CTAB binds to DNA through electrostatic interaction forming a hydrophobic complex, thus changing the micro-environment of duplex of DNA, so the binding state of MG and DNA is changed, and a complex CTAB-CT DNA-MG is formed. This fact suggests a new way to mediate the conformation of molecular assemblies of DNA and lipids.
Wang
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Z
Laboratory of Electroanalytical Chemistry and National Analytical and Research Center of Electrochemistry and Spectroscopy, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, PR China.
Liu
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D
Dong
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S
D000818:Animals; D002412:Cations; D002417:Cattle; D000077286:Cetrimonium; D002593:Cetrimonium Compounds; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D004563:Electrochemistry; D066298:In Vitro Techniques; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry*; D008739:Methyl Green / Q000737:chemistry / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D009994:Osmolar Concentration
D002412:Cations; D002593:Cetrimonium Compounds; D008055:Lipids; D008739:Methyl Green; D004247:DNA; D000077286:Cetrimonium