K-ras mutation in sputum of primary lung cancer patients does not always reflect that of cancerous cells.
K-ras mutation in sputum was examined using mutant-allele-specific amplification method among 100 primary lung cancer and 15 non-oncological patients. K-ras mutation was detected in 11 out of 59 adenocarcinoma cases (18.6%), 5 out of 32 squamous cell carcinoma cases (15.6%), 2 out of 4 large cell carcinoma cases (50.0%) and 3 out of 15 non-oncological disease cases (20.0%). In the 18 cases of primary lung cancer K-ras mutation was examined in both sputum and the resected specimen of the primary lesion. In 5 cases K-ras mutation in sputum was detected without K-ras mutation in primary lesion. Therefore, these findings suggested that K-ras mutation in sputum may not be directly related to that of the primary lesion.
Nakajima
E
E
Department of Surgery, Tokyo Medical University, Shinjuku-ku, Tokyo 160-0023, Japan. eiji1026@naa.att.ne.jp
Hirano
T
T
Konaka
C
C
Ikeda
N
N
Kawate
N
N
Ebihara
Y
Y
Kato
H
H
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D011905:Genes, ras; D005820:Genetic Testing; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009154:Mutation; D011379:Prognosis; D013183:Sputum / Q000737:chemistry* / Q000502:physiology