title: Isolation and expression of the ecdysteroid-inducible angiotensin-converting enzyme-related gene in wing discs of Bombyx mori.
abstract: We isolated a clone encoding a putative angiotensin-converting enzyme-related gene from the wing disc cDNA library of the silkworm, Bombyx mori (refer to as BmAcer). The predicted open reading frame encoded 648 amino acids with about 50% identities with the Drosophila melanogaster angiotensin-converting enzyme Ance and Acer. Northern analysis identified a 2.2-kilobase mRNA which was abundant in wing discs two days after the beginning of wandering. An accumulation of the transcript was observed approximately 2 h after 20-hydroxyecdysone (20E) exposure in vitro and was blocked slightly by a protein synthetic inhibitor. These data suggest that the transcription of the BmAcer gene is directly 20E-inducible.
authors: Faculty of Agriculture, Utsunomiya University, 350 Mine, Utsunomiya, 321-8505, Tochigi, Japan.
authors: G X
authors: GX
authors: Quan
authors: K
authors: K
authors: Mita
authors: K
authors: K
authors: Okano
authors: T
authors: T
authors: Shimada
authors: N
authors: N
authors: Ugajin
authors: Z
authors: Z
authors: Xia
authors: N
authors: N
authors: Goto
authors: E
authors: E
authors: Kanke
authors: H
authors: H
authors: Kawasaki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D029721:Drosophila Proteins; D026461:Ecdysteroids; D015870:Gene Expression; D017344:Genes, Insect; D019476:Insect Proteins / Q000235:genetics*; D008666:Metalloendopeptidases / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger; D013256:Steroids / Q000378:metabolism*; D014921:Wings, Animal
chemical_list: D018076:DNA, Complementary; D029721:Drosophila Proteins; D026461:Ecdysteroids; D019476:Insect Proteins; D012333:RNA, Messenger; D013256:Steroids; C102762:Acer protein, Drosophila; D008666:Metalloendopeptidases