title: Evaluation of a new commercial immunoassay for rapid detection of Campylobacter jejuni in stool samples.
abstract: In order to evaluate a new commercial enzyme immunoassay (ProspecT Campylobacter Microplate Assay; Alexon-Trend, USA) for the detection of Campylobacter jejuni and Campylobacter coli in stool samples, 30 faecal specimens known to be culture-positive for Campylobacter jejuni were tested with the new assay. The detection limit was approximately 3 x 10(6)/ml in faecal suspensions. The sensitivity relative to culture was 80% (24/30). All of the 24 positive samples, except for one, remained positive after being stored at -20 degrees C for 60 days. The specificity of the test was 100%. Interestingly, 6 of 11 additional Campylobacter jejuni culture-positive samples that had been obtained from patients with Guillain-Barré syndrome and stored at -20 degrees C for periods of up to 5 years tested positive in the assay. The performance of the assay indicates that it has potential value for use in future early intervention studies.
authors: Department of Medical Microbiology & Infectious Diseases, Erasmus University Medical Centre Rotterdam, The Netherlands. endtz@bacl.azr.nl
authors: H P
authors: HP
authors: Endtz
authors: C W
authors: CW
authors: Ang
authors: N
authors: N
authors: van den Braak
authors: A
authors: A
authors: Luijendijk
authors: B C
authors: BC
authors: Jacobs
authors: P
authors: P
authors: de Man
authors: J M
authors: JM
authors: van Duin
authors: A
authors: A
authors: van Belkum
authors: H A
authors: HA
authors: Verbrugh
mesh_terms: D002169:Campylobacter Infections / Q000175:diagnosis; D016123:Campylobacter jejuni / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D011933:Reagent Kits, Diagnostic
chemical_list: D011933:Reagent Kits, Diagnostic