[Is Gly460Trp variant of alpha-adducin associated with essential hypertension in the Hans of Chinese population].
OBJECTIVE
To determine whether the variant of adducin alpha subunit (alpha-adducin) is in association with essential hypertension in the Hans of Chinese population.


METHODS
One hundred and eighty-three patients with essential hypertension and 129 normotensive subjects were included in this study. Genomic DNA was isolated from 3 ml of whole blood. Polymerase chain reaction, single-strand conformational polymorphism and DNA sequencing analysis techniques were used to detect the variant in the exon 10 of alpha-adducin gene. The clinical data of height, weight and blood pressure were also measured in all subjects.


RESULTS
A G-->T substitution at nucleotide 614 in exon 10 of alpha-adducin gene which resulted in the Gly460Trp variant was found in the Hans. The distribution of Gly/Gly, Gly/Trp and Trp/Trp genotypes was coincident with Hardy-Weinberg equilibrium and there were no statistical differences in the three genotypes and Trp allele frequencies between hypertensive and normotensive subjects (P>0.05). The variant of alpha-adducin genotypes was not associated with any significant differences in age, body mass index and blood pressure.


CONCLUSIONS
A G-->T substitution at nucleotide 614 in exon 10 of alpha-adducin gene is found, but there is lack of the association between the variant of alpha-adducin gene and essential hypertension in the Hans of Chinese population.
Hou
R
R
Department of Cardiology, the First Affiliated Hospital, Xi n an Medical University, Xi n an, Shaanxi, 710061 P.R.China.
Liu
Z
Z
Xue
M
M
Wang
Y
Y
Ye
T
T
Sun
C
C
Wang
Z
Z
Liu
Y
Y
D000328:Adult; D002148:Calmodulin-Binding Proteins / Q000235:genetics*; D002681:China / Q000208:ethnology; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D006973:Hypertension / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D021122:Protein Subunits; D000099291:Adducins
D002148:Calmodulin-Binding Proteins; D021122:Protein Subunits; D000099291:Adducins