A quantitative and qualitative high performance liquid chromatographic determination of acetaminophen and five of its para-substituted derivatives.
An accurate HPLC determination of acetaminophen in tablet dosage form is reported, together with an effective separation of five of its para-substituted derivatives using an isocratic reversed-phase system. The column consisted of an octadecylsilane 10 microm stationary phase. The mobile phase was a mixture of acetonitrile and water (7:3). The retention times for acetaminophen, O-acetylacetaminophen, O-ethylacetaminophen, O-(2-nitrobenzenesulphonyl)acetaminophen, O-benzylacetaminophen and O-(3,5-dinitrobenzoyl)acetaminophen were 2.83, 3.25, 3.56. 3.78, 4.37 and 6.03 min, respectively.
Shervington
L A
LA
Pharmacy Faculty, University of Applied Science, Amman, Jordan. sher@index.com.jo
Sakhnini
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N
D000082:Acetaminophen / Q000032:analysis*; D018712:Analgesics, Non-Narcotic / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D015203:Reproducibility of Results
D018712:Analgesics, Non-Narcotic; D000082:Acetaminophen