DNA adsorption onto polyethylenimine-attached poly(p-chloromethylstyrene) beads.
In this study, DNA adsorption properties of polyethylenimine (PEI)-attached poly(p-chloromethylstyrene) (PCMS) beads were investigated. Spherical beads with an average size of 186 microm were obtained by the suspension polymerization of p-chloromethylstyrene conducted in an aqueous dispersion medium. Owing to the reasonably rough character of the bead surface, PCMS beads had a specific surface area of 14.1 m2/g. PEI chains could be covalently attached onto the PCMS beads with equilibrium binding capacities up to 208 mg PEI/g beads, via a direct chemical reaction between the amine and chloromethyl groups. After PEI adsorption with 10% (w/w) initial PEI concentration, free amino content of PEI-attached PCMS beads was determined as 0.91 mequiv./g. PEI-attached PCMS beads were utilized as sorbents in DNA adsorption experiments conducted at +4 degrees C in a phosphate buffer medium of pH 7.4. DNA immobilization capacities up to 290 mg DNA/g beads could be achieved with the tried sorbents. This value was approximately 50-times higher relative to the adsorption capacities of previously examined sorbents.
Unsal
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E
Hacettepe University, Chemical Engineering Department, Ankara, Turkey.
Bahar
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T
Tuncel
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M
Tuncel
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A
D000327:Adsorption; D004247:DNA / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D011094:Polyethyleneimine / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*
D011137:Polystyrenes; C426943:poly(p-chloromethylstyrene); D011094:Polyethyleneimine; D004247:DNA