Effect of various environmental parameters on the recovery of sublethally salt-damaged and acid-damaged Listeria monocytogenes.
The influence of supplementing the culture medium with magnesium sulphate, D-glucose, L-cysteine, catalase or lithium chloride, of incubation temperature and of oxygen availability on the recovery of salt- or acid-damaged Listeria monocytogenes, was studied on a solid repair medium according to a Hadamard matrix, with seven parameters varying between a high and a low level. The most important factors for repair of stressed Listeria were further studied with complete factorial design experiments. Results show that conditions promoting resuscitation of acid- or salt-injured cells are stress-specific, and differ in part from those described in the literature for heat-stressed Listeria.
Gnanou Besse
N
N
Agence Française de Sécurité Sanitaire des Aliments, Laboratoire d'Etudes et de Recherches sur l'Hygiène et la Qualité des Aliments, Unité Hygiène et Microbiologie des Produits Laitiers, Maisons Alfort, France.
Dubois Brissonnet
F
F
Lafarge
V
V
Leclerc
V
V
D000693:Anaerobiosis; D002374:Catalase / Q000494:pharmacology; D003470:Culture Media; D003545:Cysteine / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008089:Listeria monocytogenes / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D018021:Lithium Chloride / Q000494:pharmacology; D008278:Magnesium Sulfate / Q000494:pharmacology; D012044:Regression Analysis; D012965:Sodium Chloride / Q000494:pharmacology*; D013696:Temperature
D003470:Culture Media; D012965:Sodium Chloride; D008278:Magnesium Sulfate; D002374:Catalase; D018021:Lithium Chloride; D005947:Glucose; D003545:Cysteine