title: Cloning and expression of recombinant HEXA-HIS tagged ZAP-70 using the baculovirus system.
authors: Anti-Inflammatory Department, Roche Pharmaceuticals, Welwyn Garden City, Hertfordshire, UK.
authors: T
authors: T
authors: Purton
authors: S
authors: S
authors: Wilkinson
authors: E J
authors: EJ
authors: Murray
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016367:Baculoviridae; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005822:Genetic Vectors; D006801:Humans; D008969:Molecular Sequence Data; D010748:Phosphopeptides / Q000737:chemistry / Q000378:metabolism; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D011994:Recombinant Proteins / Q000032:analysis / Q000378:metabolism; D018411:Spodoptera; D014162:Transfection / Q000379:methods; D010948:Viral Plaque Assay; D051746:ZAP-70 Protein-Tyrosine Kinase
chemical_list: D010748:Phosphopeptides; D011948:Receptors, Antigen, T-Cell; D011994:Recombinant Proteins; D011505:Protein-Tyrosine Kinases; D051746:ZAP-70 Protein-Tyrosine Kinase; C497261:ZAP70 protein, human