A novel nucleolar G-protein conserved in eukaryotes.
We describe here a novel, evolutionarily conserved set of predicted G-proteins. The founding member of this family, TbNOG1, was identified in a two-hybrid screen as a protein that interacts with NOPP44/46, a nucleolar phosphoprotein of Trypanosoma brucei. The biological relevance of the interaction was verified by co-localization and co-immunoprecipitation. TbNOG1 localized to the trypanosome nucleolus and interacted with domains of NOPP44/46 that are found in several other nucleolar proteins. Genes encoding proteins highly related to TbNOG1 are present in yeast and metazoa, and related G domains are found in bacteria. We show that NOG1 proteins in humans and Saccharomyces cerevisae are also nucleolar. The S. cerevisae NOG1 gene is essential for cell viability, and mutations in the predicted G motifs abrogate function. Together these data suggest that NOG1 may play an important role in nucleolar functions. The GTP-binding region of TbNOG1 is similar to those of Obg and DRG proteins, which, together with NOG, form a newly recognized family of G-proteins, herein named ODN. The ODN family differs significantly from other G-protein families, and shows several diagnostic sequence characteristics. All organisms appear to possess an ODN gene, pointing to the biological significance of this family of G-proteins.
Park
J H
JH
Seattle Biomedical Research Institute, Seattle, WA 98109, USA.
Jensen
B C
BC
Kifer
C T
CT
Parsons
M
M
D000818:Animals; D019556:COS Cells; D002466:Cell Nucleolus; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D005057:Eukaryotic Cells; D019204:GTP-Binding Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D005800:Genes, Fungal; D006801:Humans; D009687:Nuclear Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics; D015800:Protozoan Proteins; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014346:Trypanosoma brucei brucei / Q000254:growth & development / Q000378:metabolism; D020798:Two-Hybrid System Techniques
C427468:NOG1 protein, Trypanosoma brucei; C100391:NOPP44-46 protein, Trypanosoma brucei; D009687:Nuclear Proteins; D010750:Phosphoproteins; D016271:Proto-Oncogene Proteins c-myc; D015800:Protozoan Proteins; D016601:RNA-Binding Proteins; D019204:GTP-Binding Proteins