title: [Neutralizing antibodies to 5 HIV-1 strains in macaques immunized with the multi-epitope polypeptide TAB9].
abstract: Presence of neutralizing antibodies(Nab) to 5 laboratory strains of human immunodeficiency virus type-1 (HIV-1) was studied and compared in the sera of eight macaca (Macaca fascicularis) after the third and fourth immunizations with multi-epitope polypeptide TAB9, emulsified with adjuvant Montanide ISA 720. Four animals were inoculated 1 mg and the others 200 micrograms, 2 animals were used as controls and injected only with adjuvant. Although the presence of neutralizing antibodies to homologous strains of HIV-group group B was confirmed in most animals after immunization through antigen p24 capture ELISA (DAVIH-Agp24, Cuba), no statistically significant differences were found neither in titers caused by antigen concentrations nor in the responses after the third and fourth immunizing doses. Controls did not develop neutralizing antibodies.
authors: Centro de Investigaciones Científicas de la Defensa Civil, San José de las Lajas, La Habana, Cuba.
authors: L M
authors: LM
authors: Navea Leyva
authors: L
authors: L
authors: Lobaina Bartelemy
authors: M
authors: M
authors: Dubed Echevarría
authors: G
authors: G
authors: Alvarez Seguí
authors: C
authors: C
authors: Gómez Rodríguez
authors: C
authors: C
authors: Duarte Cano
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes / Q000008:administration & dosage / Q000276:immunology*; D015483:HIV Antibodies / Q000097:blood*; D015488:HIV Antigens / Q000008:administration & dosage / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D007114:Immunization / Q000379:methods; D008252:Macaca fascicularis; D009500:Neutralization Tests / Q000379:methods / Q000706:statistics & numerical data; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000939:Epitopes; D015483:HIV Antibodies; D015488:HIV Antigens; D011993:Recombinant Fusion Proteins; C417682:TAB9