title: Streptococcus pneumoniae heat shock protein 70 does not induce human antibody responses during infection.
abstract: Mouse monoclonal antibodies (mAbs) were developed against Streptococcus pneumoniae in search for potential common pneumococcal proteins as vaccine antigens. mAb 230,B-9 (IgG1) reacted by immunoblotting with a 70-kDa protein which was isolated by immunoaffinity chromatography and subsequent preparative electrophoresis. N-terminal amino acid sequencing showed homology to that of heat shock protein 70 (hsp70). The hsp70 epitope reactive with mAb 230,B-9 was found in all the pneumococci examined as well as in other streptococci and enterococci. The epitope was not expressed in several other examined Gram-positive or -negative bacteria. Pneumococcal hsp70 has by other investigators been proposed to be a vaccine candidate. Binding experiments using flow cytometry showed that the epitope was not surface-exposed on live exponential phase grown S. pneumoniae. Human patient sera did not react with affinity-purified pneumococcal hsp70. Therefore the pneumococcal hsp70 does not seem to be of special interest in a vaccine formulation. The human sera contained antibodies to high molecular proteins co-purified with hsp70. Some of these proteins could be the pneumococcal surface protein A.
authors: Department of Vaccinology, National Institute of Public Health, Torshov, Oslo, Norway. jan.kolberg@folkehelsa.no
authors: J
authors: J
authors: Kolberg
authors: E A
authors: EA
authors: Høiby
authors: A
authors: A
authors: Aase
authors: K
authors: K
authors: Sletten
authors: G
authors: G
authors: Rødal
authors: T E
authors: TE
authors: Michaelsen
authors: A
authors: A
authors: Bucher
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000097:blood; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D016983:Enterococcus / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D005434:Flow Cytometry; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D051379:Mice; D008969:Molecular Sequence Data; D011008:Pneumococcal Infections / Q000276:immunology* / Q000382:microbiology; D013291:Streptococcus / Q000276:immunology; D013296:Streptococcus pneumoniae / Q000235:genetics / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D000939:Epitopes; D018840:HSP70 Heat-Shock Proteins; C051592:pneumococcal surface protein A