Determination of domoic acid in Japanese mussels by enzyme immunoassay.
Ten samples of commercial blue mussels (Mytilus edulis) from Japan were analyzed for domoic acid by an indirect competitive enzyme immunoassay (idc-EIA) based on an anti-domoic acid monoclonal antibody. Domoic acid was found in all samples at low concentrations (0.11-1.81 ng/g mussel tissue). The presence of domoic acid was confirmed by liquid chromatography coupled with immunoaffinity chromatography using an anti-domoic acid monoclonal antibody as ligand. To our knowledge, this is the first reported detection of domoic acid, a causative agent of amnesic shellfish poisoning, in Japanese mussels.
Kawatsu
K
K
Osaka Prefectural Institute of Public Health, Department of Food Microbiology, Nakamichi, Japan.
Hamano
Y
Y
Noguchi
T
T
D000818:Animals; D000911:Antibodies, Monoclonal; D049872:Bivalvia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D007124:Immunoenzyme Techniques; D007202:Indicators and Reagents; D007564:Japan; D007608:Kainic Acid / Q000031:analogs & derivatives* / Q000032:analysis*; D008387:Marine Toxins / Q000032:analysis*
D000911:Antibodies, Monoclonal; D007202:Indicators and Reagents; D008387:Marine Toxins; C012301:domoic acid; D007608:Kainic Acid