title: Chitin synthase 1 (Chs1) gene sequences of Microsporum equinum and Trichophyton equinum.
abstract: Chitin synthase 1 (Chs1) genes from Microsporum equinum and Trichophyton equinum were compared with those of the other dermatophytes. The Chs1 nucleotide sequences of these dermatophytes from horses showed more than 80% similarity to those of Arthroderma benhamiae, A. fulvum, A. grubyi, A. gypseum, A. incruvatum, A. otae, A. simii, A. vanbreuseghemii, Epidermophyton floccosum, T. mentagrophytes var. interdigitale (T. interdigitale), T. rubrum and T. violaceum. Especially high degree of nucleotide sequence similarity of more than 99% was noted between the Chs1 gene fragments of M. equinum and A. otae, and those of T. equinum, T. interdigitale and A. vanbreuseghemii, respectively. The phylogenetic analysis of their sequences revealed that M. equinum was genetically very close to A. otae and T. equinum to A. vanbreuseghemii. A molecular analysis of Chs1 genes will provide useful information for the genetic relatedness of M. equinum and T. equinum and confirm the value of DNA sequencing in identification of these two dermatophytes.
authors: Department of Pathobiology, Nihon University School of Veterinary Medicine, 1866, Kameino, Fujisawa, 252-8510, Kanagawa, Japan. kano@brs.nihon-u.ac.jp
authors: R
authors: R
authors: Kano
authors: S
authors: S
authors: Aihara
authors: Y
authors: Y
authors: Nakamura
authors: S
authors: S
authors: Watanabe
authors: A
authors: A
authors: Hasegawa
mesh_terms: D000818:Animals; D002687:Chitin Synthase / Q000235:genetics*; D003001:Cloning, Molecular; D003881:Dermatomycoses / Q000382:microbiology / Q000662:veterinary; D006734:Horse Diseases / Q000382:microbiology; D006736:Horses; D008864:Microsporum / Q000201:enzymology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary; D014249:Trichophyton / Q000201:enzymology*
chemical_list: D002687:Chitin Synthase