Standardization of capillary zone electropherograms obtained by using field enhanced sample stacking.
Migration times in a capillary zone electropherogram obtained by using the field enhanced sample stacking technique are strongly affected by the injected sample volume. That is, the migration times significantly decrease with the increase of the sample volume. To avoid inaccurate qualitative analysis due to the above phenomena, the time axis of the electropherograms was converted into an effective mobility axis using our conversion method taking account of the temperature increase in the separation tube and relaxation of the potential gradient of the separation field. After the conversion, accurate qualitative analysis was possible in spite of drastic change of the migration time, suggesting our conversion method could be successfully used for the standardization of electropherograms obtained even by using the stacking effect. The cause of the decrease of the migration time in the stacking process was briefly discussed.
Hirokawa
T
T
Applied Physics and Chemistry, Faculty of Engineering, Hiroshima University, Higashi-Hiroshima, Japan. hiro77@hiroshima-u.ac.jp
Ikuta
N
N
Yoshiyama
T
T
D019075:Electrophoresis, Capillary / Q000592:standards*