title: Tissue-specific autoregulation of Drosophila suppressor of forked by alternative poly(A) site utilization leads to accumulation of the suppressor of forked protein in mitotically active cells.
abstract: The Suppressor of forked protein is the Drosophila homolog of the 77K subunit of human cleavage stimulation factor, a complex required for the first step of the mRNA 3'-end-processing reaction. We have shown previously that wild-type su(f) function is required for the accumulation of a truncated su(f) transcript polyadenylated in intron 4 of the gene. This led us to propose a model in which the Su(f) protein would negatively regulate its own accumulation by stimulating 3'-end formation of this truncated su(f) RNA. In this article, we demonstrate this model and show that su(f) autoregulation is tissue specific. The Su(f) protein accumulates at a high level in dividing tissues, but not in nondividing tissues. We show that this distribution of the Su(f) protein results from stimulation by Su(f) of the tissue-specific utilization of the su(f) intronic poly(A) site, leading to the accumulation of the truncated su(f) transcript in nondividing tissues. Utilization of this intronic poly(A) site is affected in a su(f) mutant and restored in the mutant with a transgene encoding wild-type Su(f) protein. These data provide an in vivo example of cell-type-specific regulation of a protein level by poly(A) site choice, and confirm the role of Su(f) in regulation of poly(A) site utilization.
authors: Génétique du Développement de la Drosophile, Institut de Génétique Humaine, Montpellier, France.
authors: F
authors: F
authors: Juge
authors: A
authors: A
authors: Audibert
authors: B
authors: B
authors: Benoit
authors: M
authors: M
authors: Simonelig
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics* / Q000502:physiology; D005786:Gene Expression Regulation; D006801:Humans; D019476:Insect Proteins / Q000378:metabolism*; D007438:Introns; D007814:Larva; D008317:Malpighian Tubules / Q000502:physiology; D008938:Mitosis; D009687:Nuclear Proteins; D009928:Organ Specificity; D011061:Poly A / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D014921:Wings, Animal / Q000502:physiology; D001617:beta-Glucosidase / Q000235:genetics
chemical_list: D029721:Drosophila Proteins; D019476:Insect Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; C079745:su(f) protein, Drosophila; D011061:Poly A; D001617:beta-Glucosidase