title: Thermal injury effects on intestinal crypt cell proliferation and death are cell position dependent.
abstract: We examined the effects of thermal injury on intestinal epithelial cell proliferation and death. We recorded histologically identifiable mitotic and apoptotic crypt cells in relation to cell position after a 60% full thickness cutaneous thermal injury in the rat. The injury significantly reduced mitosis (0.53 +/- 0.11 vs. 1. 50 +/- 0.70, P < 0.05) at cell positions 4-6, stem cells, 6 h after injury. A similar reduction in mitosis (1.13 +/- 0.59 vs. 3.50 +/- 0. 80, P < 0.05) was observed at higher cell positions 7-9 12 h after injury, indicating a positional cell shift. In addition, a significant increase in the number of apoptotic bodies occurred at cell positions 7-9 (2.32 +/- 0.87 vs. 0.13 +/- 0.22, P < 0.05) and 10-12 (2.2 +/- 0.12 vs. 0.00, P < 0.05) 6 h after injury. Thermal injury-induced alterations in mitotic and apoptotic activities were transient since crypts recovered with a moderate increase in mitotic activity 24 h after injury. In control and thermal-injury rats 24 h after injury, crypt cell mitosis and apoptosis did not differ significantly. This demonstrates that cutaneous thermal injury causes a transient suppression of mitosis as well as induction of apoptosis in a cell position-dependent manner in the small intestinal crypt.
authors: Department of Surgery, Shriners Hospitals for Children and The University of Texas Medical Branch, Galveston, Texas 77555, USA.
authors: M
authors: M
authors: Varedi
authors: R
authors: R
authors: Chinery
authors: G H
authors: GH
authors: Greeley
authors: D N
authors: DN
authors: Herndon
authors: E W
authors: EW
authors: Englander
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002056:Burns / Q000473:pathology*; D002452:Cell Count; D002455:Cell Division / Q000502:physiology; D020287:In Situ Nick-End Labeling; D007413:Intestinal Mucosa / Q000293:injuries / Q000473:pathology; D007421:Intestine, Small / Q000293:injuries* / Q000473:pathology*; D008297:Male; D008938:Mitosis / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley