Lipid peroxidation induced by Clinostomum detruncatum in muscle of the freshwater fish Rhamdia quelen.
The effect of Clinostomum detruncatum metacercaria infection on the activities of the antioxidant enzymes superoxide dismutase and catalase in muscle of the freshwater fish Rhamdia quelen was analyzed. Tert-butyl hydroperoxide-initiated chemiluminescence, a measure of lipid peroxidation, was also investigated. Enzyme activities were similar in infected and uninfected fishes. However, the chemiluminescence was almost 2-fold higher in muscle of infected fishes than in muscle of uninfected ones. These results indicate that parasite infection induces oxidative stress and a higher level of membrane damage in the fish muscle due to an imbalance between pro-oxidants and non-enzymatic antioxidants. Our results suggest that fish response to parasite infection could involve, as in other vertebrates, reactive oxygen intermediates.
Belló
A R
AR
Departamento de Parasitologia, Universidade Federal do Rio Grande do Sul, Porto Alegre-RS, Brazil.
Fortes
E
E
Belló-Klein
A
A
Belló
A A
AA
Llesuy
S F
SF
Robaldo
R B
RB
Bianchini
A
A
D000818:Animals; D001938:Brazil; D002374:Catalase / Q000032:analysis; D002397:Catfishes; D005393:Fish Diseases / Q000469:parasitology* / Q000473:pathology; D015227:Lipid Peroxidation; D008163:Luminescent Measurements; D018482:Muscle, Skeletal / Q000201:enzymology / Q000469:parasitology* / Q000473:pathology; D018384:Oxidative Stress; D012588:Scintillation Counting / Q000662:veterinary; D013482:Superoxide Dismutase / Q000032:analysis; D014200:Trematoda / Q000472:pathogenicity*; D014201:Trematode Infections / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*; D020122:tert-Butylhydroperoxide / Q000737:chemistry
D020122:tert-Butylhydroperoxide; D002374:Catalase; D013482:Superoxide Dismutase