A preliminary analysis of Wuchereria bancrofti microfilarial antigens for potential use in diagnosis.
Several antigens from the microfilarial stage of Wuchereria bancrofti have been identified using immunoblots of microfilarial antigens and screening with immune sera and tropical pulmonary eosinophilia (TPE) sera. This analysis revealed an array of antigens with apparent molecular weights of 14kDa, 35kDa, 42kDa, 63kDa, 88kDa, 97kDa and 200kDa. Among these only the 14kDa and 42kDa antigens were consistently recognized by most of the immune sera. A 132kDa antigen was recognized only by TPE sera. Analysis of rabbit immune sera revealed that the 42kDa antigen was shared by two developmental stages of W. bancrofti, namely L3 and mF. This antigen could become a potential vaccine candidate. The 14kDa antigen seems specific for the microfilarial stage and therefore could be a diagnostic marker for active infection. The 132kDa antigen could aid in the diagnosis of TPE.
Saverimuttu
J K
JK
Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Colombo, Sri Lanka.
Berzins
K
K
Perlmann
P
P
Karunanayake
E H
EH
Ismail
M M
MM
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology; D003429:Cross Reactions; D005368:Filariasis / Q000175:diagnosis* / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D007106:Immune Sera; D007114:Immunization; D015151:Immunoblotting; D008842:Microfilariae / Q000276:immunology; D008970:Molecular Weight; D011657:Pulmonary Eosinophilia / Q000276:immunology; D011817:Rabbits; D014612:Vaccines / Q000276:immunology; D014958:Wuchereria bancrofti / Q000254:growth & development / Q000276:immunology*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007106:Immune Sera; D014612:Vaccines