Antisense basic fibroblast growth factor oligonucleotide reduced adhesion of retinal pigment epithelial cells to extracellular matrix molecules and their proliferation.
We investigated the effect of extracellular matrix molecules on the adhesion of retinal pigment epithelial cells and their subsequent proliferation. Fibronectin, collagen type I and vitronectin enhanced their adhesion and proliferation. In addition, the effect of basic fibroblast growth factor (bFGF) on their adhesion and proliferation was studied. bFGF enhanced their adhesion and proliferation, whereas antisense bFGF reduced their adhesion to and their proliferation on extracellular matrix molecules.
Heun Rho
S
S
Department of Ophthalmology, Dong-A University College of Medicine and Institute of Medical Science, Pusan, South Korea.
Seong Yoon
H
H
Won Yoo
K
K
Chan Park
W
W
Hee Jeong
J
J
Hyun Yoo
Y
Y
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005346:Fibroblast Growth Factors; D005455:Fluorescent Antibody Technique; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D011506:Proteins / Q000494:pharmacology*; D016372:RNA, Antisense / Q000494:pharmacology*; D013552:Swine
D016326:Extracellular Matrix Proteins; D011506:Proteins; D016372:RNA, Antisense; C108111:fibroblast growth factor 13; D005346:Fibroblast Growth Factors