title: Subsite specificity of divalent metal ions to glucosyltransferase.
abstract: Glucosyltransferase from oral bacteria Streptococcus mutans is the most significant virulent factor in causing dental caries. The enzyme has two subsites. The binding specificity of divalent metal ions to glucosyl or fructosyl subsite was examined using multiple inhibition kinetics. The interaction factor "alpha" identifies whether the two subsites are exclusive or non-exclusive.
authors: Department of Basic Sciences, University of Southern California, Los Angeles 90089-0641, USA.
authors: K S
authors: KS
authors: Devulapalle
authors: G
authors: G
authors: Mooser
mesh_terms: D001665:Binding Sites; D019187:Cadmium Compounds / Q000378:metabolism; D002413:Cations, Divalent / Q000378:metabolism*; D003035:Cobalt / Q000378:metabolism; D003300:Copper / Q000494:pharmacology; D003731:Dental Caries / Q000382:microbiology; D005296:Ferrous Compounds / Q000378:metabolism; D005964:Glucosyltransferases / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D017895:Manganese Compounds / Q000378:metabolism; D013295:Streptococcus mutans / Q000201:enzymology*; D013431:Sulfates / Q000378:metabolism; D019287:Zinc Sulfate / Q000378:metabolism
chemical_list: D019187:Cadmium Compounds; D002413:Cations, Divalent; D005296:Ferrous Compounds; D017895:Manganese Compounds; D013431:Sulfates; C020748:ferrous sulfate; D003035:Cobalt; D019287:Zinc Sulfate; D003300:Copper; C037123:cadmium sulfate; D005964:Glucosyltransferases; C018021:cobaltous chloride; C029892:cupric chloride; C039798:manganese sulfate