High-performance liquid chromatography with peroxyoxalate chemiluminescence detection of bisphenol A migrated from polycarbonate baby bottles using 4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride as a label.
A highly sensitive and selective high-performance liquid chromatographic method with peroxyoxalate chemiluminescence detection for the determination of bisphenol A at sub-ppb levels is described. Bisphenol A was derivatized with 4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride and the excess unreacted reagent was removed by a simple solid-phase extraction procedure with recoveries of approximately 60%. The separation was carried out isocratically on an ODS column and the derivatized bisphenol A was detected by peroxyoxalate chemiluminescence. A mixture of bis[2-(3,6,9-trioxadecanyloxycarbonyl)-4-nitrophenyl]oxalate (0.6 mM) and hydrogen peroxide (25.0 mM) dissolved in acetonitrile was used as a chemiluminescence reagent solution with a mixture of imidazole-HNO3 buffer (40.0 mM, pH 7.0): acetonitrile (17:83, v/v) as a mobile phase. The linear standard curve was obtained over the range from 0.57 (2.5) to 22.8 (100) ppb (nM) (r=0.996) with a detection limit of 0.38 ppb (2.8 fmol on column) at a signal-to-noise ratio of 3. The method was successfully applied to the determination of bisphenol A in hot water in contact with commercially available baby bottle samples.
Sun
Y
Y
Graduate School of Pharmaceutical Sciences, Nagasaki University, Japan.
Wada
M
M
Al-Dirbashi
O
O
Kuroda
N
N
Nakazawa
H
H
Nakashima
K
K
D001559:Benzhydryl Compounds; D001565:Benzoates / Q000737:chemistry*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007093:Imidazoles / Q000737:chemistry*; D008163:Luminescent Measurements; D010636:Phenols / Q000032:analysis*; D011076:Polycarboxylate Cement / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D001565:Benzoates; D007093:Imidazoles; D010636:Phenols; D011076:Polycarboxylate Cement; C114684:4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride; C005506:polycarbonate; C006780:bisphenol A; D000098826:Bisphenol A Compounds