title: Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12.
abstract: A novel sequence discovered in a computational screen appears distantly related to the p35 subunit of IL-12. This factor, which we term p19, shows no biological activity by itself; instead, it combines with the p40 subunit of IL-12 to form a novel, biologically active, composite cytokine, which we term IL-23. Activated dendritic cells secrete detectable levels of this complex. IL-23 binds to IL-12R beta 1 but fails to engage IL-12R beta 2; nonetheless, IL-23 activates Stat4 in PHA blast T cells. IL-23 induces strong proliferation of mouse memory (CD4(+)CD45Rb(low)) T cells, a unique activity of IL-23 as IL-12 has no effect on this cell population. Similar to IL-12, human IL-23 stimulates IFN-gamma production and proliferation in PHA blast T cells, as well as in CD45RO (memory) T cells.
authors: DNAX Research Institute, Palo Alto, CA 94304, USA.
authors: B
authors: B
authors: Oppmann
authors: R
authors: R
authors: Lesley
authors: B
authors: B
authors: Blom
authors: J C
authors: JC
authors: Timans
authors: Y
authors: Y
authors: Xu
authors: B
authors: B
authors: Hunte
authors: F
authors: F
authors: Vega
authors: N
authors: N
authors: Yu
authors: J
authors: J
authors: Wang
authors: K
authors: K
authors: Singh
authors: F
authors: F
authors: Zonin
authors: E
authors: E
authors: Vaisberg
authors: T
authors: T
authors: Churakova
authors: M
authors: M
authors: Liu
authors: D
authors: D
authors: Gorman
authors: J
authors: J
authors: Wagner
authors: S
authors: S
authors: Zurawski
authors: Y
authors: Y
authors: Liu
authors: J S
authors: JS
authors: Abrams
authors: K W
authors: KW
authors: Moore
authors: D
authors: D
authors: Rennick
authors: R
authors: R
authors: de Waal-Malefyt
authors: C
authors: C
authors: Hannum
authors: J F
authors: JF
authors: Bazan
authors: R A
authors: RA
authors: Kastelein
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D016207:Cytokines / Q000235:genetics* / Q000276:immunology; D016208:Databases, Factual; D006801:Humans; D018664:Interleukin-12 / Q000235:genetics* / Q000276:immunology; D053759:Interleukin-23; D053760:Interleukin-23 Subunit p19; D007378:Interleukins / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017421:Sequence Analysis
chemical_list: D016207:Cytokines; C508840:IL23A protein, human; C508841:Il23a protein, mouse; D053759:Interleukin-23; D053760:Interleukin-23 Subunit p19; D007378:Interleukins; D018664:Interleukin-12