Effect of azidothymidine on the radiation-induced LDH release in HeLa cells.
Treatment of HeLa cells with various concentrations of 0, 0.01, 0.1, 1, 10, and 100 microM AZT resulted in a concentration dependent elevation in the LDH release at 0, 0.5, 1, 2 and 4 h post-treatment. An elevation of 1.7 to 9.2 fold in LDH content was observed at 1 h post-treatment depending on the drug concentration. Similarly, treatment of HeLa cells with 0.1 microM AZT before irradiation caused an irradiation dose dependent increase in the LDH release in AZT + irradiation groups. This increase in LDH release was approximately two fold greater at 0 h post-irradiation in AZT + irradiation group, when compared with the PBS + irradiation group. This trend of elevation in LDH release continued up to 2 h, except 2 and 3 Gy, where it was 1.7 fold in the former group when compared with the latter. However, a peak level of LDH release was observed at 0 h post-irradiation.
Jagetia
G C
GC
Departments of Radiobiology and Clinical Biochemistry, Kasturba Medical College, Manipal, India.
Aruna
R
R
Nayak
B S
BS
D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006367:HeLa Cells; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism* / Q000528:radiation effects*; D015215:Zidovudine / Q000494:pharmacology*
D015215:Zidovudine; D007770:L-Lactate Dehydrogenase