title: Crosslinkable coatings from phosphorylcholine-based polymers.
abstract: 2-Methacryloyloxyethyl phosphorylcholine (MPC) was synthesised and then used in the preparation of crosslinked polymer membranes with lauryl methacrylate, hydroxypropyl methacrylate and trimethoxysilylpropyl methacrylate (crosslinker) comonomers. Some physical aspects of the membrane properties were evaluated in order to establish the basis for the synthesis of a series of post-crosslinkable polymers. These materials were made by copolymerisation of the constituent monomers via a free radical method, and characterised using NMR, FT-IR, viscometry and elemental analysis. The optimum crosslink density and conditions required for curing coatings of these polymers were investigated using atomic force microscopy (AFM) and showed the inclusion of 5 mol% silyl crosslinking agent to be ideal. A nanoindentation technique was employed to determine if the coating developed elasticity upon crosslinking. The biological properties of the coatings were evaluated using a variety of protein adsorption assays and blood contacting experiments, and an enzyme immunoassay was developed to detect E. coli in order to assess the level of bacterial adhesion to these biomaterials. Polymers of this type were shown to be very useful as coating materials for improving the biocompatibility of, or reducing the levels of adherent bacteria to medical devices.
authors: Research and Development Group, Biocompatibles Ltd, Farnham, Surrey, UK. andrew_lewis@biocompatibles.co.uk
authors: A L
authors: AL
authors: Lewis
authors: Z L
authors: ZL
authors: Cumming
authors: H H
authors: HH
authors: Goreish
authors: L C
authors: LC
authors: Kirkwood
authors: L A
authors: LA
authors: Tolhurst
authors: P W
authors: PW
authors: Stratford
mesh_terms: D001422:Bacterial Adhesion; D020099:Coated Materials, Biocompatible; D003432:Cross-Linking Reagents / Q000138:chemical synthesis* / Q000737:chemistry; D004926:Escherichia coli / Q000502:physiology; D006461:Hemolysis; D006801:Humans; D007850:Lauric Acids; D009682:Magnetic Resonance Spectroscopy; D008689:Methacrylates / Q000138:chemical synthesis / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D011108:Polymers; D013329:Structure-Activity Relationship; D014297:Trimethylsilyl Compounds
chemical_list: D020099:Coated Materials, Biocompatible; D003432:Cross-Linking Reagents; D007850:Lauric Acids; D008689:Methacrylates; D011108:Polymers; D014297:Trimethylsilyl Compounds; C421257:poly(lauryl methacrylate); C421258:poly(trimethoxysilylpropyl methacrylate); D010767:Phosphorylcholine; C070638:2-methacryloyloxyethyl phosphorylcholine; C032802:hydroxypropyl methacrylate