Isolation of Vibrio alginolyticus from seawater aquaria.
The seawater bacterium Vibrio alginolyticus was detected in 5 of 20 water samples from seawater aquaria (from 3 of 5 units) and also from the surface of diseased stony corals. A total of 45 isolates were differentiated biochemically, of which 13 isolates (29%) proved to be V. alginolyticus. All those strains produced the virulence factors caseinase and lipase, 11 strains amylase and gelatinase. 7 strains showed lecithinase activity and 2 strains produced hemolysins. All examined strains showed a marked toxicity to vero cells proven by the MTT-bioassay, but no toxicity to plant cells with the saline alga Asteromonas gracilis as model. The isolates were mostly resistant to beta-lactam antibiotics, macrolides and lincomycin. However, they proved to be susceptible to aminoglycoside- and polypeptide-antibiotics as well as to tetracyclines, chloramphenicol, florfenicol, enrofloxacin and sulfamethoxazol-trimethoprim. The possible participation of this bacterium in the bleaching and dying of stony corals is mentioned as well as its role as human pathogen.
Hörmansdorfer
S
S
Technische Universität München, Germany.
Wentges
H
H
Neugebaur-Büchler
K
K
Bauer
J
J
D000818:Animals; D003063:Cnidaria / Q000382:microbiology; D004352:Drug Resistance, Microbial; D006801:Humans; D012623:Seawater / Q000382:microbiology*; D014733:Vibrio / Q000737:chemistry / Q000201:enzymology / Q000302:isolation & purification* / Q000472:pathogenicity*; D014871:Water Microbiology