Evidence of tissue-specific, post-transcriptional regulation of NRF-2 expression.
Mitochondrial respiratory function requires the expression of genes both from the mitochondrial and nuclear genomes. Nuclear respiratory factor 2 (NRF-2) is a transcription factor required for the expression of several nuclear-encoded mitochondrial proteins, including the specific mitochondrial transcription factor Tfam. This makes NRF-2 a likely candidate to coordinate expression of mitochondrial components. NRF-2 is a multisubunit complex of which the alpha subunit binds DNA and the beta subunit enhances this binding, respectively. We have analysed in vivo the expression patterns of NRF-2 subunits both at the mRNA and protein level, in three rat tissues, liver, testis and brain. In contrast with Tfam or the 'housekeeping' beta-actin expressions in which a parallel gradient was observed, no correlation was found between NRF-2 mRNAs and proteins levels, thus suggesting post-transcriptional regulation.
Vallejo
C G
CG
Instituto de Investigaciones Biomédicas 'Alberto Sols' (CSIC-UAM), Arturo Duperier, 4, 28029, Madrid, Spain. cvallejo@iib.uam.es
Escrivá
H
H
Rodríguez-Peña
A
A
D000199:Actins / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000502:physiology; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D051268:GA-Binding Protein Transcription Factor; D005786:Gene Expression Regulation; D008099:Liver / Q000502:physiology; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D009928:Organ Specificity; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013737:Testis / Q000502:physiology; D015534:Trans-Activators; D014157:Transcription Factors / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D029867:Xenopus Proteins
D000199:Actins; D004268:DNA-Binding Proteins; D051268:GA-Binding Protein Transcription Factor; C495649:Gabpa protein, rat; C495653:Gabpb2 protein, rat; D012333:RNA, Messenger; C489030:Tfam protein, rat; D015534:Trans-Activators; D014157:Transcription Factors; C097078:XL-MTTFA protein, Xenopus; D029867:Xenopus Proteins