title: Association between favorable neuroblastoma and high expression of the novel metalloproteinase gene, nbla3145/XCE, cloned by differential screening of the full-length-enriched oligo-capping neuroblastoma cDNA libraries.
abstract: BACKGROUND
  The biology of neuroblastoma (NBL) is at least partly regulated by neurotrophic factors and their receptors.
  
  
  METHODS
  To identify novel NBL-related genes that affect growth, differentiation, and programmed cell death of the tumor, we constructed full-length-enriched cDNA libraries by oligo-capping method.
  
  
  RESULTS
  Semi-quantitative and quantitative real-time RT-PCR showed that the nbla3145 gene was significantly highly expressed in favorable subset of NBL. The nucleo-tide sequence of nbla3145 revealed that it was a novel member of metalloproteinase, endothelin-converting enzyme, and was the same gene recently reported as XCE. We also cloned nbla3145beta which was a novel truncated form of nbla3145 (or nbla3145alpha).
  
  
  CONCLUSIONS
  Our results suggested that nbla3145/XCE plays an important role in regulating growth and differentiation of NBL.
authors: Division of Biochemistry, Chiba Cancer Center Research Institute, Chiba, Japan.
authors: T
authors: T
authors: Kawamoto
authors: T
authors: T
authors: Shishikura
authors: M
authors: M
authors: Ohira
authors: H
authors: H
authors: Takayasu
authors: A
authors: A
authors: Morohashi
authors: N
authors: N
authors: Takada
authors: M
authors: M
authors: Takahashi
authors: Y
authors: Y
authors: Suzuki
authors: S
authors: S
authors: Sugano
authors: T
authors: T
authors: Hori
authors: A
authors: A
authors: Nakagawara
mesh_terms: D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D006801:Humans; D008666:Metalloendopeptidases / Q000235:genetics*; D009447:Neuroblastoma / Q000235:genetics*
chemical_list: C492359:ECEL1 protein, human; D008666:Metalloendopeptidases