In vitro selection of hematoporphyrin binding DNA aptamers.
DNA aptamers that bind to hematoporphyrin IX (HPIX) were isolated using an in vitro selection technique. Most aptamers obtained after the 7th and 10th rounds contained guanine-rich sequences. Binding assay using fluorescence polarization technique and structural analysis by CD spectra revealed that the parallel guanine-quartet structure of the aptamer participates in the recognition of HPIX.
Okazawa
A
A
Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Japan.
Maeda
H
H
Fukusaki
E
E
Katakura
Y
Y
Kobayashi
A
A
D001483:Base Sequence; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D005454:Fluorescence Polarization; D006415:Hematoporphyrins / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011485:Protein Binding
D006415:Hematoporphyrins; D004247:DNA