title: High-speed pectic enzyme fractionation by immobilised metal ion affinity membranes.
abstract: Immobilised metal ion affinity polysulfone hollow-fibre membranes, with a high capacity for protein adsorption, were prepared and their utilisation for commercial pectic enzyme fractionation was studied. The pass-through fraction containing pectinlyase is useful for fruit-juice clarification without methanol production on account of pectinesterase being retained by the IDA-Cu2+ membrane.
authors: Cátedra de Microbiología Industrial y Biotecnología, Facultad de Farmacia y Bioquímica, UBA, Argentina. scamperi@huemul.ffyb.uba.ar
authors: S A
authors: SA
authors: Camperi
authors: M
authors: M
authors: Grasselli
authors: O
authors: O
authors: Cascone
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods*; D003300:Copper; D007098:Imino Acids; D011096:Polygalacturonase / Q000302:isolation & purification*; D011133:Polysaccharide-Lyases / Q000302:isolation & purification*
chemical_list: D007098:Imino Acids; D003300:Copper; D011096:Polygalacturonase; D011133:Polysaccharide-Lyases; C027349:pectin lyase; C008109:iminodiacetic acid