title: EGF regulates early embryonic mouse gut development in chemically defined organ culture.
abstract: The profound intestinal epithelial defects in the newborn epidermal growth factor receptor (EGFR) knockout mouse suggests that EGFR signaling plays important roles in embryonic gut development. Herein, we further elucidated the function of EGFR signaling on early embryonic gut development by comparing the effects of 1-10 ng/mL of exogenous epidermal growth factor (EGF) or 10-25 microM of the tyrphostin 3,4,5 trihydroxybenzene malononitrile, a specific inhibitor of EGFR tyrosine kinase, on intact E12 Swiss-Webster mouse midgut grown in chemically defined organ culture using Fitton-Jackson BGJb medium for 4 or 6 d. Intestinal development during culture was assayed by morphometry, histology, reverse transcription/competitive PCR for villin and intestinal fatty acid binding protein mRNA, and immunohistochemistry for epithelial proliferative markers. During organ culture, control specimens grew in length, developed smooth muscle, simple columnar epithelial and goblet cell phenotypes, showed early villus formation in the proximal intestine, and increased expression of villin and intestinal fatty acid binding protein mRNA. EGF failed to significantly alter small intestinal lengthening, whereas EGF 10 ng/mL inhibited colonic length growth. Tyrphostin 25 microM resulted in regional losses of stromal and smooth muscle cells in the small intestine and absent colonic goblet cells. In controls, cellular proliferation initially occurred throughout the small intestinal epithelium but became increasingly localized to the intervillus crypt regions. This sequestration of epithelial proliferation into crypts was much more apparent in EGF-treated versus tyrphostin-treated specimens. EGFR activation, therefore, appears to accelerate the maturation rate of goblet cells and the differential crypt/villus proliferation pattern in early embryonic mouse gut.
authors: Division of Gastroenterology and Nutrition, Department of Pediatrics, Children's Hospital Los Angeles, University of Southern California Keck School of Medicine, 90027, USA.
authors: G
authors: G
authors: Duh
authors: N
authors: N
authors: Mouri
authors: D
authors: D
authors: Warburton
authors: D W
authors: DW
authors: Thomas
mesh_terms: D000818:Animals; D003106:Colon / Q000187:drug effects / Q000196:embryology / Q000648:ultrastructure; D016895:Culture Media, Serum-Free; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D066246:ErbB Receptors / Q000187:drug effects / Q000502:physiology; D005260:Female; D005326:Fetal Proteins / Q000187:drug effects / Q000502:physiology; D007091:Image Processing, Computer-Assisted; D007413:Intestinal Mucosa / Q000187:drug effects / Q000196:embryology / Q000648:ultrastructure; D007422:Intestines / Q000187:drug effects / Q000196:embryology*; D051379:Mice; D008871:Microvilli / Q000187:drug effects; D009924:Organ Culture Techniques; D011247:Pregnancy; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D015398:Signal Transduction; D020032:Tyrphostins / Q000494:pharmacology
chemical_list: D016895:Culture Media, Serum-Free; D005326:Fetal Proteins; D018809:Proliferating Cell Nuclear Antigen; D020032:Tyrphostins; D004815:Epidermal Growth Factor; D066246:ErbB Receptors