Structure of the VPATPD gene encoding subunit D of the human vacuolar proton ATPase.
The HSD11B2 and VPATPD genes encoding the human kidney isozyme of 11beta-hydroxysteroid dehydrogenase (11-HSD2) and subunit D of the vacuolar proton ATPase, respectively, are located on chromosome 16q22. They are transcribed from complementary DNA strands and their 3' ends are only 0.5 kilobase apart. Because polymorphisms in HSD11B2 have been associated with hypertension and salt sensitivity, we characterized the human VPATPD gene. It spans 19 kb and consists of 8 exons. The encoded protein is 99.5% identical to mouse subunit D at the amino acid level. An alternating purine-pyrimidine tract is located in the 3'-untranslated region of VPATPD. On genotyping 17 hypertensive subjects, no length polymorphism was found. Although VPATPD and HSD11B2 are both expressed in kidney and placenta, they are regulated differently; forskolin upregulates HSD11B2 but not VPATPD in human choriocarcinoma JEG3 cells. The functional significance of the proximity of these two genes remains to be established.
Agarwal
A K
AK
Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas, 75390-9063, USA.
White
P C
PC
D042842:11-beta-Hydroxysteroid Dehydrogenases; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002822:Choriocarcinoma; D005091:Exons; D006801:Humans; D006913:Hydroxysteroid Dehydrogenases / Q000235:genetics; D007438:Introns; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics; D007668:Kidney / Q000201:enzymology; D051379:Mice; D008969:Molecular Sequence Data; D021122:Protein Subunits; D017494:Proton Pumps / Q000737:chemistry / Q000235:genetics; D006180:Proton-Translocating ATPases / Q000737:chemistry / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D025262:Vacuolar Proton-Translocating ATPases; D001613:beta 2-Microglobulin / Q000235:genetics
D007527:Isoenzymes; D021122:Protein Subunits; D017494:Proton Pumps; D001613:beta 2-Microglobulin; D006913:Hydroxysteroid Dehydrogenases; D042842:11-beta-Hydroxysteroid Dehydrogenases; D025262:Vacuolar Proton-Translocating ATPases; D006180:Proton-Translocating ATPases