title: Synthesis of alpha-Gal epitope derivatives with a galactosyltransferase-epimerase fusion enzyme.
abstract: Alpha-Gal epitopes are carbohydrate structures bearing an alpha-D-Galp-(1-->3)-beta-D-Galp terminus and are the main cause of antibody-mediated hyperacute rejection in xenotransplantation. Nine monosaccharides and ten disaccharides were evaluated as substrates for a fusion protein, which contains both alpha-(1-->3)-galactosyltransferase and uridine-5'-diphosphogalactose 4-epimerase. Four disaccharide and six trisaccharide alpha-Gal epitope derivatives were synthesized utilizing this novel fusion enzyme.
authors: Department of Chemistry, Wayne State University, Detroit, MI 48202-3489, USA.
authors: J
authors: J
authors: Fang
authors: X
authors: X
authors: Chen
authors: W
authors: W
authors: Zhang
authors: A
authors: A
authors: Janczuk
authors: P G
authors: PG
authors: Wang
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005700:Galactosyltransferases / Q000235:genetics / Q000378:metabolism; D006084:Graft Rejection / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009844:Oligosaccharides / Q000138:chemical synthesis / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000138:chemical synthesis / Q000378:metabolism; D014183:Transplantation, Heterologous; D014312:Trisaccharides / Q000138:chemical synthesis / Q000276:immunology*; D014534:UDPglucose 4-Epimerase / Q000235:genetics / Q000378:metabolism
chemical_list: D009844:Oligosaccharides; D011993:Recombinant Fusion Proteins; D014312:Trisaccharides; C076569:alpha-galactosyl epitope; D005700:Galactosyltransferases; D014534:UDPglucose 4-Epimerase