title: Cloning of 17beta-hydroxysteroid dehydrogenase-I cDNAs from Japanese eel ovary.
abstract: 17beta-hydroxysteroid dehydrogenase-I (17beta-HSD-I) is a key steroidogenic enzyme for estradiol-17beta (E(2)) production. cDNAs encoding 17beta-HSD-I were cloned for the first time in lower vertebrates from the ovary of a teleost, the Japanese eel. The deduced amino acid sequence from these cDNAs was approximately 50% identical to mammalian 17beta-HSD-Is. 17beta-HSD-I mRNA was not detected in previtellogenic ovaries by Northern blotting. However, transcript abundance increased in early vitellogenic ovaries obtained from fish artificially matured by gonadotropic treatment, but thereafter did not appear to change further. Recombinant 17beta-HSD-I expressed in human kidney 293 cells selectively converted estrone to E(2), but androstenedione, testosterone, or E(2) were not converted to any other steroids. Although it is widely accepted that E(2) is produced from testosterone in other species of teleosts, the substrate specificity of eel 17beta-HSD-I suggests that a steroidogenic pathway for production of E(2) from androstenedione via estrone exists in the Japanese eel ovary.
authors: Division of Marine Biosciences, Graduate School of Fisheries Sciences, Hokkaido University, 3-1-1 minato-cho, Hakodate, Hokkaido, 041-8611, Japan. kazeto@umbi.umd.edu
authors: Y
authors: Y
authors: Kazeto
authors: S
authors: S
authors: Ijiri
authors: H
authors: H
authors: Matsubara
authors: S
authors: S
authors: Adachi
authors: K
authors: K
authors: Yamauchi
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002645:Chickens; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004524:Eels / Q000235:genetics*; D005260:Female; D006801:Humans; D007564:Japan; D008297:Male; D008322:Mammals; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010053:Ovary / Q000201:enzymology*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D013737:Testis / Q000201:enzymology; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D011994:Recombinant Proteins; D015067:17-Hydroxysteroid Dehydrogenases; C044256:3 (or 17)-beta-hydroxysteroid dehydrogenase