[Immunogenic activity of temperature sensitive mutation of herpes simplex virus type-1 in mono- and polyvalent conditions with different attenuated strains of virus. I. Analysis of humoral immunity induced by mutant temperature sensitive herpes simplex virus type-1 in monovalent systems].
The aim of the present study was the analysis of the immunogenic activity of temperature sensitive mutant of HSV-1 with significantly decreased pathogenicity for mice. The stability of immunogenic potency was also tested. The level of antibody-mediated response in guinea pigs after two doses of mutant ts HSV-1 or native strain was compared. Antibodies to HSV were measured using ELISA and CFM. Seroconversion rates in tested groups were high, from 80% to 100%. The level of antibody response at 4 week after inoculation measured by ELISA and CFM showed no significant differences between 4 passages of mutant ts HSV-1. Significant higher increase of antibodies level after two doses of viruses was observed for mutant ts HSV-1 as compared to native strain, both in ELISA and CFM.
Biesiadecka
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Zakład Wirusologii Państwowego Zakładu Higieny w Warszawie.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000917:Antibody Formation / Q000276:immunology*; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000821:virology; D006168:Guinea Pigs; D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000276:immunology* / Q000472:pathogenicity; D009154:Mutation; D013696:Temperature
D000914:Antibodies, Viral