Transfection of the plasma-type platelet-activating factor acetylhydrolase gene attenuates glutamate-induced apoptosis in cultured rat cortical neurons.
Using an adenoviral vector, we induced overexpression of the plasma type of platelet-activating factor acetylhydrolase in cultured rat neurons. Neurons overexpressing this enzyme showed a decrease in glutamate-induced injury, mainly, apparent as decreased apoptosis. Reduction of lipid peroxidation by this enzyme and protection of mitochondrial function were demonstrated, and these may be the basis of the resistance to glutamate-induced neuronal injury that we observed.
Hirashima
Y
Y
Department of Neurosurgery, Toyama Medical and Pharmaceutical University, Toyama-shi, 930-0194, Toyama, Japan. yhira@ms.toyama-mpu.ac.jp
Ueno
H
H
Karasawa
K
K
Yokoyama
K
K
Setaka
M
M
Takaku
A
A
D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D005822:Genetic Vectors; D018698:Glutamic Acid / Q000494:pharmacology; D006168:Guinea Pigs; D007763:Lac Operon; D008315:Malondialdehyde / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000201:enzymology*; D010741:Phospholipases A / Q000235:genetics*; D051381:Rats; D014162:Transfection
D018698:Glutamic Acid; D008315:Malondialdehyde; D010741:Phospholipases A; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase