Changes in electrophysiological properties of cat hypoglossal motoneurons during carbachol-induced motor inhibition.
The control of hypoglossal motoneurons during sleep is important from a basic science perspective as well as to understand the bases for pharyngeal occlusion which results in the obstructive sleep apnea syndrome. In the present work, we used intracellular recording techniques to determine changes in membrane properties in adult cats in which atonia was produced by the injection of carbachol into the pontine tegmentum (AS-carbachol). During AS-carbachol, 86% of the recorded hypoglossal motoneurons were found to be postsynaptically inhibited on the basis of analyses of their electrical properties; the electrical properties of the remaining 14% were similar to motoneurons recorded during control conditions. Those cells that exhibited changes in their electrical properties during AS-carbachol also displayed large-amplitude inhibitory synaptic potentials. Following sciatic nerve stimulation, hypoglossal motoneurons which responded with a depolarizing potential during control conditions exhibited a hyperpolarizing potential during AS-carbachol. Both spontaneous and evoked inhibitory potentials recorded during AS-carbachol were comparable to those that have been previously observed in trigeminal and spinal cord motoneurons under similar experimental conditions as well as during naturally occurring active sleep. Calculations based on modeling the changes that we found in input resistance and membrane time constant with a three-compartment neuron model suggest that shunts are present in all three compartments of the hypoglossal motoneuron model. Taken together, these data indicate that postsynaptic inhibitory drives are widely distributed on the soma-dendritic tree of hypoglossal motoneurons during AS-carbachol. These postsynaptic inhibitory actions are likely to be involved in the pathophysiology of obstructive sleep apnea.
Fung
S J
SJ
Department of Physiology, UCLA School of Medicine, 53-263 CHS, Los Angeles, CA 90095, USA. sjfung@ucla.edu
Yamuy
J
J
Xi
M C
MC
Engelhardt
J K
JK
Morales
F R
FR
Chase
M H
MH
D000818:Animals; D002217:Carbachol / Q000494:pharmacology*; D002415:Cats; D018679:Cholinergic Agonists / Q000494:pharmacology*; D003712:Dendrites / Q000187:drug effects / Q000502:physiology; D007002:Hypoglossal Nerve / Q000187:drug effects* / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D008959:Models, Neurological; D009046:Motor Neurons / Q000187:drug effects* / Q000502:physiology; D009433:Neural Inhibition / Q000187:drug effects* / Q000502:physiology; D012895:Sleep, REM / Q000502:physiology
D018679:Cholinergic Agonists; D002217:Carbachol