P0 protein peptide 180-199 together with pertussis toxin induces experimental autoimmune neuritis in resistant C57BL/6 mice.
The C57BL/6 mice strain is known to be reputedly resistant to induction of experimental autoimmune neuritis (EAN), an animal model of Guillain-Barré syndrome in human by bovine peripheral myelin (BPM), and P2 protein or the P2 protein peptide 57-81. The P0 peptide 180-199 is a stronger neuritogenic antigen than the P2 peptide 57-81. We found that this synthetic peptide induced both clinical and pathological characteristics of an acute monophasic EAN in C57BL/6 mice. Only male mice were more sensitive to EAN induction with the P0 peptide 180-199. Intravenously administrated pertussis toxin (PT) had an adjuvant effect that increased the incidence of P0 peptide 180-199-induced EAN as well as the inflammation and demyelination in the peripheral nerves. Spontaneous and P0 peptide 180-199 stimulated proliferation of peripheral T-cells were enhanced by PT-treatment as well. The enhancing effect was lower before onset of the disease (Day 6 post immunization) (p.i.) as compared to the early phase of the disease (Day 22 p.i.). Thus, P0 peptides together with PT are able to break tolerance to myelin in C57BL/6 mice.
Zou
L P
LP
Division of Geriatric Medicine, Karolinska Institute, Huddinge University Hospital, Stockholm, Sweden.
Ljunggren
H G
HG
Levi
M
M
Nennesmo
I
I
Wahren
B
B
Mix
E
E
Winblad
B
B
Schalling
M
M
Zhu
J
J
D000276:Adjuvants, Immunologic; D000818:Animals; D007249:Inflammation / Q000473:pathology; D008198:Lymph Nodes / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D018993:Myelin P0 Protein / Q000276:immunology*; D009186:Myelin Sheath / Q000473:pathology; D009444:Neuritis, Autoimmune, Experimental / Q000139:chemically induced* / Q000473:pathology; D010446:Peptide Fragments / Q000276:immunology*; D037342:Pertussis Toxin; D012584:Sciatic Nerve / Q000473:pathology; D013601:T-Lymphocytes / Q000473:pathology; D010566:Virulence Factors, Bordetella / Q000276:immunology*
D000276:Adjuvants, Immunologic; D018993:Myelin P0 Protein; D010446:Peptide Fragments; D010566:Virulence Factors, Bordetella; D037342:Pertussis Toxin