title: Magnetic susceptibility shift selected imaging (MESSI) and localized (1)H(2)O spectroscopy in living plant tissues.
abstract: Maize root segments permeated with aqueous solutions of the paramagnetic agents GdDTPA(2-) or DyDTPA-BMA display two well-resolved NMR peaks corresponding to the signals from intracellular and extracellular (1)H(2)O, which arise from well-understood bulk magnetic susceptibility effects. This allows each component to be studied separately. Images obtained at each frequency with MESSI editing, and single- and multiple-voxel ('spectroscopic imaging') localized spectra, clearly indicate that the agents permeate into the interstitial spaces, and into the longitudinal (xylem/phloem) channels in the stele (core) of the root, confirming earlier assessments. We believe these are the first images of a multicellular tissue acquired in vivo exclusively from the intracellular water proton resonance. This method can be further exploited to study water transport in similar systems.
authors: Department of Chemistry, SUNY Stony Brook, NY 11794-3400, USA.
authors: K
authors: K
authors: Zhong
authors: X
authors: X
authors: Li
authors: Y
authors: Y
authors: Shachar-Hill
authors: F
authors: F
authors: Picart
authors: A
authors: A
authors: Wishnia
authors: C S
authors: CS
authors: Springer
mesh_terms: D001692:Biological Transport / Q000502:physiology; D008279:Magnetic Resonance Imaging / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy; D018517:Plant Roots / Q000378:metabolism; D014867:Water / Q000737:chemistry / Q000378:metabolism*; D003313:Zea mays / Q000378:metabolism*
chemical_list: D014867:Water