Genetic diversity in Proteus mirabilis isolates found in the urinary tract of rheumatoid arthritis patients.
OBJECTIVE
Elevated levels of anti-Proteus antibodies but not antibodies to E. coli have been reported in patients with rheumatoid arthritis (RA). The suggestion has been made that P. mirabilis may have a role in the aetiopathogenesis of rheumatoid arthritis. The aim of this study was to determine whether there are differences at the genetic level inisolates of P. mirabilis obtained from controls and RA patients.


METHODS
A blind study was performed whereby P. mirabilis isolates obtained from urinary cultures of RA patients and controls were analysed using RAPD PCR. Isolates were then grouped on the basis of their DNA band profile after agarose gel electrophoresis, thereby allowing the composition of the Proteus population in the urinary tract to be analysed at the genetic level.


RESULTS
Fourteen different DNA band profiles were obtained from the 93 isolates tested: 70% of these isolates fell into only five of the 14 groups and approximately 25% of all isolates fell into one group. No differences were observed in the frequency of isolates from either control or RA subjects.


CONCLUSIONS
There is genetic diversity in P. mirabilis populations found in the urinary tract, but there are no differences in the frequency of these bacteria between RA patients and controls.
Whiteford
J R
JR
Department of Molecular Genetics, Imperial College, Exhibition Road, London, South Kensington, UK.
Wilson
C
C
Tiwana
H
H
Ebringer
A
A
D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D005260:Female; D014644:Genetic Variation; D006801:Humans; D008297:Male; D008875:Middle Aged; D011513:Proteus mirabilis / Q000235:genetics* / Q000302:isolation & purification; D019105:Random Amplified Polymorphic DNA Technique; D014551:Urinary Tract / Q000382:microbiology*; D014556:Urine / Q000382:microbiology*
D004269:DNA, Bacterial