Inhibitory effect of intestinal anti-Gb3 IgA antibody on verotoxin-induced cytotoxicity.
The effect of intestinal IgA antibody against the receptor for verotoxin (VT), globotriaosylceramide (Gb3), on VT-mediated cytotoxicity was examined. Intestinal IgA antibodies against Gb3 were prepared by oral immunization of mice with Gb3 and adjuvant monophosphoryl lipid A (MPL)-containing liposomes composed of dipalmitoylphosphatidylcholine, dipalmitoylphosphatidylserine and cholesterol (1 : 1 : 2, molar ratio) (PS-liposome). Oral administration with Gb3 and MPL-containing PS-liposome induced significant IgA responses to Gb3 in the intestinal lavage fluid in all mice tested. Furthermore, anti-Gb3 IgA antibodies in the lavage fluid effectively inhibited the cytotoxicity of VT2 to Vero cells in a dose-dependent manner. These results suggest that anti-Gb3 IgA antibodies produced in the intestinal tract, upon oral immunization with Gb3-containing liposome, function as inhibitors against VT and also indicate the potential usefulness of oral PS-liposome vaccines containing MPL for the induction of a protective mucosal immune response against intestinal diseases.
Watarai
S
S
Laboratory of Veterinary Immunology, Department of Veterinary Science, College of Agriculture, Osaka Prefecture University, Sakai, Osaka, Japan. swatarai@vet.osakafu-u.ac.jp
Tana
Inoue
K
K
Oguma
K
K
Naka
K
K
Kodama
H
H
D000818:Animals; D002522:Chlorocebus aethiops; D003603:Cytotoxins / Q000633:toxicity*; D005260:Female; D007070:Immunoglobulin A / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011956:Receptors, Cell Surface / Q000276:immunology*; D022603:Shiga Toxins / Q000633:toxicity*; D018675:Toxicity Tests; D014281:Trihexosylceramides / Q000276:immunology*; D014709:Vero Cells
D003603:Cytotoxins; D007070:Immunoglobulin A; D011956:Receptors, Cell Surface; D022603:Shiga Toxins; D014281:Trihexosylceramides; C018549:globotriaosylceramide