The DREAM-DRE interaction: key nucleotides and dominant negative mutants.
Transcriptional repressor DREAM, an EF-hand containing calcium-binding protein, blocks basal expression of target genes through specific interaction with DRE sites in the DNA. The sequence GTCA forms the central core of the DRE site, whereas flanking nucleotides contribute notably to the affinity for DREAM. Release of binding of DREAM from the DRE results in derepression, a process that is regulated by Ca(2+). Change of two amino acids within an EF-hand in DREAM blocks Ca(2+)-induced derepression and results in potent dominant negative mutants of endogenous DREAM.
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Departamento Biología Molecular y Celular, L115, Centro Nacional de Biotecnología, C.S.I.C., Campus Cantoblanco, 28049, Madrid, Spain.
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Carrión
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Echeverria
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Mellström
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Naranjo
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D000818:Animals; D001665:Binding Sites; D022762:CCAAT-Enhancer-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D002460:Cell Line; D004268:DNA-Binding Proteins; D020832:EF Hand Motifs; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D051605:Kv Channel-Interacting Proteins; D009154:Mutation; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors; D014162:Transfection; D014407:Tumor Cells, Cultured; D051840:Y-Box-Binding Protein 1
D022762:CCAAT-Enhancer-Binding Proteins; D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; C496749:KCNIP3 protein, human; D051605:Kv Channel-Interacting Proteins; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; D051840:Y-Box-Binding Protein 1; C070856:YBX1 protein, human; D002118:Calcium