Differential interleukin-8 response of intestinal epithelial cell line to reactogenic and nonreactogenic candidate vaccine strains of Vibrio cholerae.
In this study, we analyzed whether attachment of Vibrio cholerae vaccine strains to human intestinal epithelial cells can induce an interleukin-8 (IL-8) response. The IL-8 transcripts were detected by PCR amplification of reverse-transcribed mRNA, and the gene product secretion was measured by an enzyme-linked immunosorbent assay. Infection of monolayers of the undifferentiated HT29-18N2 cell line with reactogenic (JBK70 and 81) and nonreactogenic (CVD103HgR and 638) vaccine strains of V. cholerae resulted in markedly higher IL-8 expression by epithelial cells exposed to reactogenic strains than by cells exposed to the nonreactogenic strains. Additionally, epithelial cells produced IL-8 transcripts following stimulation with cholera vaccine strains in a concentration-dependent manner. These results represent a new insight into the inflammatory component of reactogenicity and could be used as a predictive marker of vaccine reactogenicity prior to human testing.
Rodríguez
B L
BL
Departamento de Genética, Centro Nacional de Investigaciones Científicas, La Habana, Cuba. boris@biocnic.cneuro.edu.cu
Rojas
A
A
Campos
J
J
Ledon
T
T
Valle
E
E
Toledo
W
W
Fando
R
R
D001422:Bacterial Adhesion; D022121:Cholera Vaccines; D019073:HT29 Cells; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics*; D007413:Intestinal Mucosa / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014734:Vibrio cholerae / Q000502:physiology*
D022121:Cholera Vaccines; D016209:Interleukin-8; D012333:RNA, Messenger