title: Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III.
abstract: Genome sequencing projects generate a wealth of information; however, the ultimate goal of such projects is to accelerate the identification of the biological function of genes. This creates a need for comprehensive studies to fill the gap between sequence and function. Here we report the results of a functional genomic screen to identify genes required for cell division in Caenorhabditis elegans. We inhibited the expression of approximately 96% of the approximately 2,300 predicted open reading frames on chromosome III using RNA-mediated interference (RNAi). By using an in vivo time-lapse differential interference contrast microscopy assay, we identified 133 genes (approximately 6%) necessary for distinct cellular processes in early embryos. Our results indicate that these genes represent most of the genes on chromosome III that are required for proper cell division in C. elegans embryos. The complete data set, including sample time-lapse recordings, has been deposited in an open access database. We found that approximately 47% of the genes associated with a differential interference contrast phenotype have clear orthologues in other eukaryotes, indicating that this screen provides putative gene functions for other species as well.
authors: Max-Planck-Institute for Cell Biology and Genetics, Dresden, Germany. Pierre.Gonczy@isrec.unil.ch
authors: P
authors: P
authors: Gönczy
authors: C
authors: C
authors: Echeverri
authors: K
authors: K
authors: Oegema
authors: A
authors: A
authors: Coulson
authors: S J
authors: SJ
authors: Jones
authors: R R
authors: RR
authors: Copley
authors: J
authors: J
authors: Duperon
authors: J
authors: J
authors: Oegema
authors: M
authors: M
authors: Brehm
authors: E
authors: E
authors: Cassin
authors: E
authors: E
authors: Hannak
authors: M
authors: M
authors: Kirkham
authors: S
authors: S
authors: Pichler
authors: K
authors: K
authors: Flohrs
authors: A
authors: A
authors: Goessen
authors: S
authors: S
authors: Leidel
authors: A M
authors: AM
authors: Alleaume
authors: C
authors: C
authors: Martin
authors: N
authors: N
authors: Ozlü
authors: P
authors: P
authors: Bork
authors: A A
authors: AA
authors: Hyman
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000502:physiology; D002455:Cell Division / Q000235:genetics*; D002875:Chromosomes; D017238:Genes, Helminth; D023281:Genomics; D016366:Open Reading Frames; D018510:RNA, Helminth
chemical_list: D018510:RNA, Helminth