Iron(II)-mimosine catalyzed cleavage of DNA.
Mimosine, DNA breaKs, Free Radicals, Fenton Reaction Supercoiled plasmid DNA was treated in vitro with H2O2, DTT and either Fe (II), Fe (II)-EDTA or Fe (II)-mimosine. The rate of DNA break formation was followed by the conversion of the supercoiled form into relaxed-circular and linear forms. In the concentration interval of 0-4 microM Fe (II), Fe (II)-EDTA slowed-down the formation of DNA breaks, while Fe (II)-mimosine enhanced the rate of break formation up to several times. A conclusion is drawn that this enhancement is due to the increased affinity of the Fe (II)-mimosine complex to DNA.
Mikhailov
I
I
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.
Ninova
P
P
Russev
G
G
Anachkova
B
B
D004249:DNA Damage; D004278:DNA, Superhelical / Q000187:drug effects*; D004492:Edetic Acid / Q000494:pharmacology; D005296:Ferrous Compounds / Q000494:pharmacology*; D007700:Kinetics; D008898:Mimosine / Q000494:pharmacology*; D010957:Plasmids / Q000187:drug effects*
D004278:DNA, Superhelical; D005296:Ferrous Compounds; C055526:Fe(II)-EDTA; D008898:Mimosine; D004492:Edetic Acid