Improvement of Schwann cell attachment and proliferation on modified hyaluronic acid strands by polylysine.
Hyaluronic acid (HyA) has the intrinsic ability to promote cell proliferation and reduce scar formation. However, the clinical use of HyA has so far been limited because of its water solubility and nonadhesive characteristics. Increasing interest in HyA as a clinically useful biomaterial has prompted our study of altering HyA's physical properties to render it a potential component of nerve grafts. In this study, strands of HyA were cross-linked by glutaraldehyde (Glut), coated with polylysine, and then inoculated with Schwann cells (SCs). Results in vivo and in vitro demonstrated that cross-linked HyA strands were water insoluble and thus less biodegradable. Poly-D-lysine-resurfaced strands showed significant SC attachment of 350-400 cells/mm(2), compared to uncoated controls (0-10 cells/mm(2), p < 0.01). Fibroblast control groups showed an attachment of 40-100 cells/mm(2) on coated strands. Immunostaining for proliferating cells showed SCs as and fibroblasts as +. Cells neither adhered to nor proliferated on the modified HyA strands that were not resurfaced. The results suggest that polylysine promotes SC attachment and proliferation to glutaraldehyde-cross-linked HyA strands, the product being a three-dimensional composite with low solubility that may have potential application in nerve grafts.
Hu
M
M
Functional Restoration Department, Stanford University, Medical School, Stanford, California, USA. minhu@hotmail.com
Sabelman
E E
EE
Tsai
C
C
Tan
J
J
Hentz
V R
VR
D000818:Animals; D002234:Carbodiimides / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003432:Cross-Linking Reagents / Q000737:chemistry; D005976:Glutaral / Q000737:chemistry; D006820:Hyaluronic Acid / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D011107:Polylysine / Q000737:chemistry / Q000494:pharmacology*; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D009418:S100 Proteins / Q000032:analysis; D012583:Schwann Cells / Q000737:chemistry / Q000166:cytology / Q000187:drug effects* / Q000502:physiology
D002234:Carbodiimides; D003432:Cross-Linking Reagents; D018809:Proliferating Cell Nuclear Antigen; D009418:S100 Proteins; C074139:1-ethyl-3-(3-dimethylaminoethyl)carbodiimide; D011107:Polylysine; D006820:Hyaluronic Acid; D005976:Glutaral