Differential inhibitory effects of sophoricoside analogs on bioactivity of several cytokines.
Effects of sophoricoside and its analogs on proinflammatory cytokines have been investigated. Sophoricoside, genistein and orobol exhibited inhibitory effects on IL-5, IL-3, GM-CSF and IL-6 bioactivities. Genistin showed inhibitory effects on IL-5 and IL-3 bioactivities, but did not inhibit GM-CSF and IL-6 bioactivities. None of the sophoricoside analogs showed inhibitory effects on both IL-1beta and TNF-alpha bioactivities. Among the compounds, sophoricoside exhibited the highest inhibitory effects on IL-5, IL-3 and IL-6 bioactivities with IC50 values of 1.9 microM, 6.9 microM and 6.0 microM, respectively and orobol did show on GM-CSF bioactivity with an IC50 value of 18.0 microM. The result would provide an additional mechanism by which the compounds exert immunosuppressive and anti-inflammatory effects.
Yun
J
J
College of Pharmacy, Chungbuk National University, Cheongju, Korea.
Lee
C K
CK
Chang
I M
IM
Takatsu
K
K
Hirano
T
T
Min
K R
KR
Lee
M K
MK
Kim
Y
Y
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects; D001578:Benzopyrans / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D016207:Cytokines / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D005419:Flavonoids / Q000494:pharmacology*; D019833:Genistein / Q000494:pharmacology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D007377:Interleukin-3 / Q000494:pharmacology; D015848:Interleukin-5 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D007529:Isoflavones / Q000494:pharmacology*; D004915:Leukemia, Erythroblastic, Acute; D008545:Melanoma; D051379:Mice; D011994:Recombinant Proteins / Q000494:pharmacology; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D001578:Benzopyrans; D016207:Cytokines; D005419:Flavonoids; D007375:Interleukin-1; D007377:Interleukin-3; D015848:Interleukin-5; D015850:Interleukin-6; D007529:Isoflavones; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; C120642:sophoricoside; C040641:genistin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D019833:Genistein; C059811:orobol