title: Up-regulation of CCR5 expression in the placenta is associated with human immunodeficiency virus-1 vertical transmission.
abstract: The role of placenta in vertical transmission is not yet fully understood. A protective role of the placenta during gestation is suggested by the finding that caesarian sections reduce the risk of transmission of human immunodeficiency virus (HIV)-1 from mother to child three- to fourfold. Here we investigated whether the immunological milieu of the placenta might be important in HIV-1 transmission. In situ imaging of immunohistochemically stained placenta sections and reverse transcriptase-polymerase chain reaction demonstrated a fourfold increase in CCR5:CXCR4 expression ratio in placentae from transmitting women compared to placentae from nontransmitting women. This chemokine receptor repertoire was consistent with an up-regulation of interleukin-4 and interleukin-10 expression in placentae from nontransmitting placentae compared to transmitting placentae. In situ imaging demonstrated that CCR5 and CXCR4 were expressed on placental macrophages and lymphocytes but not in trophoblasts. Simultaneous immunofluorescence/ultrasensitive in situ hybridization for HIV-1 gag-pol mRNA revealed that HIV-1 infects primarily CXCR4-expressing cells in placentae from nontransmitting women whereas predominantly CCR5-expressing cells were infected in placentae from transmitting women. These data are consistent with transmission of a homogeneous population of nonsyncytium-inducing HIV-1 isolates that use CCR5 as co-receptor.
authors: Department of Medicine, Division of Infectious Diseases, Karolinska Institutet Huddinge University Hospital, Stockholm, Sweden.
authors: H
authors: H
authors: Behbahani
authors: E
authors: E
authors: Popek
authors: P
authors: P
authors: Garcia
authors: J
authors: J
authors: Andersson
authors: A L
authors: AL
authors: Spetz
authors: A
authors: A
authors: Landay
authors: Z
authors: Z
authors: Flener
authors: B K
authors: BK
authors: Patterson
mesh_terms: D016207:Cytokines / Q000378:metabolism; D005260:Female; D015658:HIV Infections / Q000635:transmission*; D015497:HIV-1; D006801:Humans; D018445:Infectious Disease Transmission, Vertical; D010920:Placenta / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D019713:Receptors, CCR5 / Q000378:metabolism*; D019718:Receptors, CXCR4 / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D016207:Cytokines; D019713:Receptors, CCR5; D019718:Receptors, CXCR4