Light-induced conformational changes of rhodopsin probed by fluorescent alexa594 immobilized on the cytoplasmic surface.
A novel fluorescence method has been developed for detecting the light-induced conformational changes of rhodopsin and for monitoring the interaction between photolyzed rhodopsin and G-protein or arrestin. Rhodopsin in native membranes was selectively modified with fluorescent Alexa594-maleimide at the Cys(316) position, with a large excess of the reagent Cys(140) that was also derivatized. Modification with Alexa594 allowed the monitoring of fluorescence changes at a red excitation light wavelength of 605 nm, thus avoiding significant rhodopsin bleaching. Upon absorption of a photon by rhodopsin, the fluorescence intensity increased as much as 20% at acidic pH with an apparent pK(a) of approximately 6.8 at 4 degrees C, and was sensitive to the presence of hydroxylamine. These findings indicated that the increase in fluorescence is specific for metarhodopsin II. In the presence of transducin, a significant increase in fluorescence was observed. This increase of fluorescence emission intensity was reduced by addition of GTP, in agreement with the fact that transducin enhances the formation of metarhodopsin II. Under conditions that favored the formation of a metarhodopsin II-Alexa594 complex, transducin slightly decreased the fluorescence. In the presence of arrestin, under conditions that favored the formation of metarhodopsin I or II, a phosphorylated, photolyzed rhodopsin-Alexa594 complex only slightly decreased the fluorescence intensity, suggesting that the cytoplasmic surface structure of metarhodopsin II is different in the complex with arrestin and transducin. These results demonstrate the application of Alexa594-modified rhodopsin (Alexa594-rhodopsin) to continuously monitor the conformational changes in rhodopsin during light-induced transformations and its interactions with other proteins.
Imamoto
Y
Y
Graduate School of Materials Science, Nara Institute of Science and Technology, Ikoma, Nara 630-0101, Japan. imamoto@ms.aist-nara.ac.jp
Kataoka
M
M
Tokunaga
F
F
Palczewski
K
K
D000595:Amino Acid Sequence; D000818:Animals; D019393:Arrestin / Q000378:metabolism; D001667:Binding, Competitive; D002417:Cattle; D002462:Cell Membrane; D003545:Cysteine / Q000737:chemistry; D005456:Fluorescent Dyes; D006863:Hydrogen-Ion Concentration; D008027:Light; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009930:Organic Chemicals; D010766:Phosphorylation; D010782:Photolysis; D011485:Protein Binding; D011487:Protein Conformation / Q000528:radiation effects; D017433:Protein Structure, Secondary; D012243:Rhodopsin / Q000737:chemistry* / Q000528:radiation effects*; D013696:Temperature; D015293:Transducin / Q000378:metabolism
C417664:Alexa594; D019393:Arrestin; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D012243:Rhodopsin; D015293:Transducin; D003545:Cysteine