Genetic analyses of NFKB1 and OCA-B function: defects in B cells, serum IgM level, and antibody responses in Nfkb1-/-Oca-b-/- mice.
Defined patterns of gene expression during cell differentiation are likely to be ensured by multiple factors playing redundant roles. By generating mice deficient in both NFKB1 and OCA-B, we show here that the two transcription factors are required for B-1 cell differentiation and serum IgM production. In addition, relative to Nfkb1(-/-) or Oca-b(-/-) mice, the Nfkb1(-/-)Oca-b(-/-) mice show a decrease in conventional B cell frequencies in the spleen and augmented reductions in T-independent and T-dependent Ab responses. These results suggest that NFKB1 and OCA-B play compensatory roles in multiple aspects of B cell differentiation.
Kim
U
U
Laboratory of Biochemistry and Molecular Biology, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.
Gunther
C S
CS
Roeder
R G
RG
D000818:Animals; D000955:Antigens, T-Independent / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D003433:Crosses, Genetic; D005362:Ficoll / Q000031:analogs & derivatives* / Q000276:immunology; D006433:Hemocyanins / Q000276:immunology; D007075:Immunoglobulin M / Q000097:blood*; D007136:Immunoglobulins / Q000096:biosynthesis*; D018655:Lymphocyte Count; D008231:Lymphopenia / Q000235:genetics / Q000276:immunology / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D016328:NF-kappa B / Q000172:deficiency* / Q000235:genetics* / Q000502:physiology; D052002:NF-kappa B p50 Subunit; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology; D013154:Spleen / Q000276:immunology / Q000473:pathology; D015534:Trans-Activators / Q000172:deficiency* / Q000235:genetics* / Q000502:physiology
C042158:2,4-dinitrophenyl keyhole limpet hemocyanin; D000955:Antigens, T-Independent; C031007:DNP-Ficoll; D007075:Immunoglobulin M; D007136:Immunoglobulins; D016328:NF-kappa B; D052002:NF-kappa B p50 Subunit; C490424:Pou2af1 protein, mouse; D012333:RNA, Messenger; D015534:Trans-Activators; D005362:Ficoll; D006433:Hemocyanins