title: Rediscovery of Anopheles (Cellia) clowi (Diptera: Culicidae), a rarely recorded member of the Anopheles punctulatus group.
abstract: Anopheline specimens collected in Papua New Guinea were morphologically identified as the rarely recorded Anopheles clowi Rozeboom & Knight. Amplification of the rDNA ITS2 region of this material revealed a fragment of 750 bp confirming its placement in the Anopheles punctulatus group. This group contains 12 species and includes the major malaria vectors in the islands of the southwest Pacific. Digestion of the ITS2 with the restriction enzyme MspI produced restriction fragment-length polymorphism with bands at 380, 300, and 150 bp, a pattern shared by no other members of this group. Phylogenetic analysis involving the sequencing of a 2 kb region of the rDNA 18S gene indicated that An. clowi was monophyletic and basal to the rest of the group and showed considerable independent evolution from the other members. This is the first record of An. clowi in Papua New Guinea and only the third collection of this species since its discovery in 1945.
authors: Australian Army Malaria Institute, Weary Dunlop Drive, Gallipoli Barracks, Enoggera, Queensland 4052, Australia.
authors: R D
authors: RD
authors: Cooper
authors: D G
authors: DG
authors: Waterson
authors: M J
authors: MJ
authors: Bangs
authors: N W
authors: NW
authors: Beebe
mesh_terms: D000818:Animals; D000852:Anopheles / Q000145:classification* / Q000235:genetics; D001483:Base Sequence; D018076:DNA, Complementary; D021903:DNA, Ribosomal Spacer / Q000032:analysis / Q000145:classification; D006801:Humans; D008969:Molecular Sequence Data; D009512:New Guinea; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S; D017423:Sequence Analysis, RNA
chemical_list: D018076:DNA, Complementary; D021903:DNA, Ribosomal Spacer; D012337:RNA, Ribosomal, 18S