p29, a novel GCIP-interacting protein, localizes in the nucleus.
GCIP, a newly identified cyclin D-interacting protein, was found to reduce the phosphorylation of retinoblastoma protein and inhibit E2F1-mediated transcriptional activity. To explore more GCIP interacting proteins, the yeast two-hybrid screening using GCIP as a bait protein was performed. One novel gene, p29, was demonstrated to associate with GCIP in the yeast two-hybrid method and in vitro GST pull-down assay. Multiple tissue Northern blot analysis showed that p29 was abundantly expressed in the heart, skeletal muscle, and kidney relative to other tissues. The transient expression of HA-tagged p29 in HeLa cells localized in the nucleus. Taken together, we have isolated a novel protein, p29, which may be involved in the functional regulation of GCIP.
Chang
M S
MS
Department of Medical Research, Mackay Memorial Hospital, Taipei, Taiwan.
Chang
C L
CL
Huang
C J
CJ
Yang
Y C
YC
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D056741:Cyclin D; D016213:Cyclins / Q000378:metabolism; D005982:Glutathione Transferase / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D016366:Open Reading Frames; D014176:Protein Biosynthesis; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
C485815:CCNDBP1 protein, human; D056741:Cyclin D; D016213:Cyclins; C418608:GCIP-interacting protein p29, human; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D014157:Transcription Factors; D005982:Glutathione Transferase