title: Supermolecular structure of photosystem II and location of the PsbS protein.
abstract: This paper addresses the question of whether the PsbS protein of photosystem two (PS II) is located within the LHC II PS II supercomplex for which a three-dimensional structure has been obtained by cryoelectron microscopy and single particle analysis. The PsbS protein has recently been implicated as the site for non-photochemical quenching. Based both on immunoblotting analyses and structural considerations of an improved model of the spinach LHC II PS II supercomplex, we conclude that the PsbS protein is not located within the supercomplex. Analyses of other fractions resulting from the solubilization of PS Il-enriched membranes derived from spinach suggest that the PsbS protein is located in the LHC II-rich regions that interconnect the supercomplex within the membrane.
authors: Department of Biochemistry, Laboratories, Imperial College of Science, Technology and Medicine, London, UK.
authors: J
authors: J
authors: Nield
authors: C
authors: C
authors: Funk
authors: J
authors: J
authors: Barber
mesh_terms: D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry / Q000378:metabolism*; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D011487:Protein Conformation; D018724:Spinacia oleracea
chemical_list: D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D010940:Plant Proteins