Diagnosis and monitoring of hepatic injury. I. Performance characteristics of laboratory tests.
OBJECTIVE
To review information on performance characteristics for tests that are commonly used to identify acute and chronic hepatic injury.


METHODS
A MEDLINE search was performed for key words related to hepatic tests, including quality specifications, aminotransferases, alkaline phosphatase, gamma-glutamyltransferase, bilirubin, albumin, ammonia, and viral markers. Abstracts were reviewed, and articles discussing performance of laboratory tests were selected for review. Additional articles were selected from the references. Guideline Preparation and Review: Drafts of the guidelines were posted on the Internet, presented at the AACC Annual Meeting in 1999, and reviewed by experts. Areas requiring further amplification or literature review were identified for further analysis. Specific recommendations were made based on analysis of published data and evaluated for strength of evidence and clinical impact. The drafts were also reviewed by the Practice Guidelines Committee of the American Association for the Study of Liver Diseases and approved by the committee and the Association's Council.


CONCLUSIONS
Although many specific recommendations are made in the guidelines, some summary recommendations are discussed here. Alanine aminotransferase is the most important test for recognition of acute and chronic hepatic injury. Performance goals should aim for total error of <10% at the upper reference limit to meet clinical needs in monitoring patients with chronic hepatic injury. Laboratories should have age-adjusted reference limits for enzymes in children, and gender-adjusted reference limits for aminotransferases, gamma-glutamyltransferase, and total bilirubin in adults. The international normalized ratio should not be the sole method for reporting results of prothrombin time in liver disease; additional research is needed to determine the reporting mechanism that best correlates with functional impairment. Harmonization is needed for alanine aminotransferase activity, and improved standardization for hepatitis C viral RNA measurements.
Dufour
D R
DR
Pathology and Laboratory Medicine Service, Veterans Affairs Medical Center, Washington, DC 20422, USA. d.robert.dufour@med.va.gov
Lott
J A
JA
Nolte
F S
FS
Gretch
D R
DR
Koff
R S
RS
Seeff
L B
LB
D000208:Acute Disease; D015415:Biomarkers / Q000032:analysis; D002908:Chronic Disease; D019411:Clinical Laboratory Techniques / Q000592:standards; D006801:Humans; D008107:Liver Diseases / Q000175:diagnosis* / Q000503:physiopathology; D008111:Liver Function Tests; D016239:MEDLINE; D017410:Practice Guidelines as Topic; D011786:Quality Control
D015415:Biomarkers