Cutting edge: chromatin remodeling at the IL-4/IL-13 intergenic regulatory region for Th2-specific cytokine gene cluster.
During the differentiation of naive Th cells into Th2 effector cells, the entire IL-4/IL-13 locus is remodeled into an accessible chromatin conformation. Here we show that ectopic expression and activation of Stat6 or GATA-3 in Th cells developing under Th1-polarizing conditions lead to the induction of chromatin remodeling not only at the flanking regions of the IL-4 and IL-13 genes but also at the IL-4/IL-13 intergenic regulatory region for the IL-4/IL-13/IL-5 gene cluster. Furthermore, we demonstrate that GATA-3 and another Th2-specific, inducible protein complex interact with the IL-4/IL-13 intergenic DNase I hypersensitive region specifically in Th2 cells.
Takemoto
N
N
Department of Molecular and Developmental Biology, The Institute of Medical Science, University of Tokyo, Tokyo, Japan.
Kamogawa
Y
Y
Jun Lee
H
H
Kurata
H
H
Arai
K I
KI
O'Garra
A
A
Arai
N
N
Miyatake
S
S
D000818:Animals; D001483:Base Sequence; D002843:Chromatin / Q000378:metabolism*; D017124:Conserved Sequence; D016207:Cytokines / Q000235:genetics; D004268:DNA-Binding Proteins / Q000502:physiology; D050990:GATA3 Transcription Factor; D005786:Gene Expression Regulation / Q000276:immunology*; D006801:Humans; D018793:Interleukin-13 / Q000235:genetics* / Q000378:metabolism; D015847:Interleukin-4 / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000276:immunology*; D012045:Regulatory Sequences, Nucleic Acid; D050800:STAT6 Transcription Factor; D015398:Signal Transduction / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology / Q000378:metabolism*; D015534:Trans-Activators / Q000502:physiology; D020506:Untranslated Regions / Q000235:genetics*
D002843:Chromatin; D016207:Cytokines; D004268:DNA-Binding Proteins; D050990:GATA3 Transcription Factor; C494749:GATA3 protein, human; C494748:Gata3 protein, mouse; D018793:Interleukin-13; D050800:STAT6 Transcription Factor; C494101:STAT6 protein, human; C494100:Stat6 protein, mouse; D015534:Trans-Activators; D020506:Untranslated Regions; D015847:Interleukin-4