title: Differential scanning calorimetry of albumin solders: interspecies differences and fatty acid binding effects on protein denaturation.
abstract: OBJECTIVE
  Understanding albumin solder denaturation is important for laser tissue soldering. Human (HSA), bovine (BSA), porcine (PSA), and canine (CSA) albumin both fatty acid containing (FAC) and fatty acid free (FAF) were evaluated by using differential scanning calorimetry (DSC).
  
  
  METHODS
  DSC was used to measure difference thermograms to determine the irreversible thermal denaturation profile for 50% albumin solutions. The denaturation transition's onset, end and peak temperatures, and enthalpy were measured.
  
  
  RESULTS
  All FAC species, except BSA, exhibited twin peaked endotherms. Single endotherms were observed for all FAF species and BSA-FAC. Onset and end temperatures were significantly [P < 0.001] lower for all FAF species (except BSA's end temperature). There was a 30% decrease in the denaturation enthalpy between FAF and FAC groups.
  
  
  CONCLUSIONS
  FAF albumin solders were found to denature at significantly lower temperatures, while also having a 30% reduction in enthalpy when compared with their FAC counterparts.
authors: Center for Pediatric Urology, Department of Urology, New York Presbyterian Hospital-Weill Medical College of Cornell University, New York 10021, USA.
authors: C B
authors: CB
authors: Bleustein
authors: M
authors: M
authors: Sennett
authors: R T
authors: RT
authors: Kung
authors: D
authors: D
authors: Felsen
authors: D P
authors: DP
authors: Poppas
authors: R B
authors: RB
authors: Stewart
mesh_terms: D000818:Animals; D002152:Calorimetry, Differential Scanning; D002417:Cattle; D004285:Dogs; D005227:Fatty Acids / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D017075:Laser Coagulation; D011489:Protein Denaturation; D012709:Serum Albumin / Q000737:chemistry; D013045:Species Specificity; D013552:Swine; D013696:Temperature; D014014:Tissue Adhesives / Q000737:chemistry*
chemical_list: D005227:Fatty Acids; D012709:Serum Albumin; D014014:Tissue Adhesives