title: Interferon-gamma and sinusoidal electric fields signal by modulating NAD(P)H oscillations in polarized neutrophils.
abstract: Metabolic activity in eukaryotic cells is known to naturally oscillate. We have recently observed a 20-s period NAD(P)H oscillation in neutrophils and other polarized cells. Here we show that when polarized human neutrophils are exposed to interferon-gamma or to ultra-low-frequency electric fields with periods double that of the NAD(P)H oscillation, the amplitude of the NAD(P)H oscillations increases. Furthermore, increases in NAD(P)H amplitude, whether mediated by interferon-gamma or by an oscillating electric field, signals increased production of reactive oxygen metabolites. Hence, amplitude modulation of NAD(P)H oscillations suggests a novel signaling mechanism in polarized cells.
authors: Department of Biological Sciences, Wayne State University, Detroit, Michigan 48202, USA. arosensp@sun.science.wayne.edu
authors: A J
authors: AJ
authors: Rosenspire
authors: A L
authors: AL
authors: Kindzelskii
authors: H R
authors: HR
authors: Petty
mesh_terms: D048429:Cell Size; D004558:Electric Stimulation; D006801:Humans; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000494:pharmacology*; D007700:Kinetics; D009243:NAD / Q000097:blood*; D009249:NADP / Q000097:blood*; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D009991:Oscillometry; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D009243:NAD; D009249:NADP; D007371:Interferon-gamma