title: Distinct distribution of rare US genotypes of Kaposi's sarcoma-associated herpesvirus (KSHV) in South Texas: implications for KSHV epidemiology.
abstract: Genotypes of Kaposi's sarcoma (KS)-associated herpesvirus (KSHV) from patients with KS in South Texas were examined. Open-reading frame (ORF)-K1 and ORF-K15 DNA segments from 16 KSHV isolates were amplified by polymerase chain reaction, and KSHV subtypes were assigned on the basis of sequence variations. K1 genotyping showed that 75% exhibited C subtype and 25% exhibited A subtype. K15 genotyping showed that 56% exhibited M form, of which 89% exhibited C3 K1 subtype and 44% exhibited P form. A unique isolate was found and was classified as C6 clade. All of the M KSHV isolates had been obtained from human immunodeficiency virus-negative classic KS patients >50 years of age, of whom 78% were Hispanic. Conversely, all KS patients with AIDS were <36 years of age and exhibited P form KSHV. These findings indicate that C3/M KSHV genotypes are more prevalent in South Texas (50%) than in other US regions (3%) and that M form KSHV likely existed in this region long before the AIDS epidemic.
authors: Department of Pediatrics, The University of Texas Health Science Center at San Antonio, San Antonio, Texas 78229-3900, USA.
authors: Y J
authors: YJ
authors: Zhang
authors: T L
authors: TL
authors: Davis
authors: X P
authors: XP
authors: Wang
authors: J H
authors: JH
authors: Deng
authors: J
authors: J
authors: Baillargeon
authors: I T
authors: IT
authors: Yeh
authors: H B
authors: HB
authors: Jenson
authors: S J
authors: SJ
authors: Gao
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D005838:Genotype; D018023:HIV Seronegativity; D006679:HIV Seropositivity; D006566:Herpesviridae Infections / Q000453:epidemiology / Q000821:virology*; D019288:Herpesvirus 8, Human / Q000145:classification / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016366:Open Reading Frames; D010802:Phylogeny; D015995:Prevalence; D012514:Sarcoma, Kaposi / Q000821:virology*; D013781:Texas / Q000453:epidemiology