Improved performances and control of beer fermentation using encapsulated alpha-acetolactate decarboxylase and modeling.
The use of the enzyme alpha-acetolactate decarboxylase allows the acceleration of beer fermentation/maturation because it shunts diacetyl formation, whose elimination is the rate-limiting step of the process. To obtain a cost reduction by using this exogenous enzyme, we propose a new process involving recoverable encapsulated alpha-acetolactate decarboxylase. The performance of traditional and new processes was investigated by a modeling approach. A simple model, focused on alpha-acetolactate and diacetyl profiles during beer fermentation, was set up. The simulated profiles are consistent with literature data. This study shows also that encapsulated alpha-acetolactate decarboxylase allows the acceleration of beer fermentation as efficiently as free alpha-acetolactate decarboxylase. The advantage of immobilized alpha-acetolactate decarboxylase versus free enzyme is that it is recoverable and reusable, which means a process cost reduction.
Dulieu
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C
LFBI, ENSAIA, 2 avenue de la Forêt de Haye, BP 172, F-54505 Vandoeuvre les Nancy Cedex, France.
Moll
M
M
Boudrant
J
J
Poncelet
D
D
D000094:Acetoin Dehydrogenase / Q000378:metabolism; D001515:Beer; D050260:Carbohydrate Metabolism; D002262:Carboxy-Lyases / Q000378:metabolism*; D005285:Fermentation; D005511:Food Handling; D007700:Kinetics; D008956:Models, Chemical
D000094:Acetoin Dehydrogenase; D002262:Carboxy-Lyases; C023746:acetolactate decarboxylase