Detection and elimination of contaminating microorganisms in transplantable tumors and cell lines.
As a quarantine of biological materials, we tested 96 transplantable tumors and cell lines for contamination with microorganisms in a mouse antibody production (MAP) test, enzymatic assay and microbiological culture. Contamination with lactic dehydrogenase elevating virus (LDV), mycoplasmas and Pasteurella pneumotropica was detected. A considerable difference in the contamination rate was observed between in vivo- and in vitro- propagated tumors. LDV in the tumors could be eliminated by both in vitro subculture and subpassage in nude rats. Mycoplasmas were eliminated by means of the mycoplasma-removal agent and P. pneumotropica by subpassage in mice. These results suggest that there is still a high risk of contamination in transplantable tumors and emphasizes the importance of adequate microbiological quality control.
Nakai
N
N
Developmental Research Laboratory, Nippon Shinyaku Co., Ltd., Kyoto, Japan.
Kawaguchi
C
C
Nawa
K
K
Kobayashi
S
S
Katsuta
Y
Y
Watanabe
M
M
D000818:Animals; D000917:Antibody Formation; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009174:Mycoplasma / Q000145:classification / Q000276:immunology / Q000302:isolation & purification; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000382:microbiology* / Q000821:virology*; D010325:Pasteurella / Q000145:classification / Q000276:immunology / Q000302:isolation & purification; D011786:Quality Control; D051381:Rats; D011923:Rats, Nude; D014407:Tumor Cells, Cultured / Q000382:microbiology*; D014780:Viruses / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification