Molecular cloning and expression of the nucleoside deoxyribosyltransferase-II gene from Lactobacillus helveticus.
Nucleoside deoxyribosyltransferase-II, which catalyzes transfer of glycosyl residues from a donor deoxynucleoside to an acceptor base, was purified from Lactobacillus helveticus and its gene was cloned. Analysis of the nucleotide sequence showed the presence of a 474-nucleotide open reading frame encoding a protein of 158 amino acids with a molecular weight of 18,317. The active enzyme can be produced in large quantities in E. coli cells using the cloned gene.
Okuyama
K
K
Biochemicals Division, Yamasa Corporation, Choshi, Chiba, Japan. okuyama@yamasa.com
Noguchi
T
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D004269:DNA, Bacterial; D007778:Lactobacillus / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D010430:Pentosyltransferases / Q000235:genetics* / Q000378:metabolism
D017931:DNA Primers; D004269:DNA, Bacterial; D010430:Pentosyltransferases; C420226:nucleoside deoxyribosyltransferase II