Determination of purines including 2,8-dihydroxyadenine in urine using capillary electrophoresis.
For the purpose of rapid drug monitoring, methods have been developed for the determination of 2,8-dihydroxyadenine, allopurinol, oxypurinol, adenine, hypoxanthine, hippuric acid and xanthine in urine with and without sodium dodecyl sulfate as additive in sodium tetraborate running buffer. No sample preparation is necessary. 6-methylmercaptopurine and etofylline have been used as the internal standards. The limit of detection is 5 microM and the range of quantification stretches from 20 to 2000 microM. The capillary electrophoresis methods are simple, fast and robust.
Wessel
T
T
Abt Hämatologie/Onkologie, Universiäts-Kinderklinik, Münster, Germany.
Lanvers
C
C
Fruend
S
S
Hempel
G
G
D000225:Adenine / Q000031:analogs & derivatives* / Q000652:urine*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D011687:Purines / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D011687:Purines; C002993:2,8-dihydroxyadenine; D000225:Adenine