Orientation of bull sperms in static magnetic fields.
The orientation of bull sperm cells in static magnetic fields was investigated by microscopic observation. The bull sperm, which has a very flat head, was fixed and its motion was stopped by glutaraldehyde. It was oriented with the whole body and the flat head perpendicular to the direction of the magnetic field. The diamagnetic cell components, such as the cell membrane, the DNA in the head, and the microtubule in the tail, were thought to contribute to this orientation, because bull sperm does not have paramagnetic components. For quantitative measurement of the orientation, the intensity of transmitted light through glutaraldehyde-fixed bull sperm suspension in a photometric cell was determined. The intensity changed slightly in proportion to the mean degree of orientation of the sperms. It increased sigmoidally depending on the intensity of the magnetic field and reached 100% at just below 1 T. The magnetic orientation is very strong in comparison to that of erythrocytes or platelets. By studying the response of the bull sperm to the magnetic field, it will be possible to understand its microstructure in more detail.
Emura
R
R
School of Allied Health Sciences, Faculty of Medicine, Osaka University, Osaka, Japan. emura@sahs.med.osaka-u.ac.jp
Ashida
N
N
Higashi
T
T
Takeuchi
T
T
D000818:Animals; D002417:Cattle; D004574:Electromagnetic Fields; D066298:In Vitro Techniques; D008297:Male; D009949:Orientation; D013081:Sperm Motility / Q000528:radiation effects; D013094:Spermatozoa / Q000502:physiology* / Q000528:radiation effects / Q000648:ultrastructure