title: Transperitoneal transport of glucose in vitro.
abstract: The effect of fluid mixing intensification, damage of mesothelial cells, gentamicin, and icodextrin on the diffusive glucose transport across the peritoneal membrane were evaluated in in vitro studies. A mathematical model of mass transport was used to calculate the diffusive permeability, expressed as a diffusive permeability coefficient (P). In the control conditions, the rate of glucose transfer from the interstitial to the mesothelial side of membrane (I-->M) and in the opposite direction (M-->I) remained constant, and the P value at mean was 2,731 +/- 1,493 x 10-4 (cm x s-1). The change of the stirring rate from 5.5 to 11 ml/min increased P values by about 74% for transport direction I-->M and 58% for M-->I, and the change from 11 to 22 ml/min enhanced P at mean by about 42% for both directions. The damage of the mesothelial layer, using sodium deoxycholate (2.5 mmol/L; 103.6 mg%), increased the glucose transfer from the interstitial to the mesothelial side of the peritoneum by 41% and to the opposite direction by 70%. Addition of icodextrin to the glucose solution increased glucose bidirectional transport at mean by about 14% for I-->M and 24% for M-->I. Furthermore, gentamicin did not change the I-->M transfer, but diminished M-->I transport by about 12%. In conclusion, the reduction of unstirred fluid layers at the mesothelium and the interstitium-fluid interfaces, removal of mesothelium, and addition of icodextrin increased the diffusive glucose transport in vitro; unstirred fluid layers restricted glucose transfer (I-->M) more than the mesothelium; and peritoneal glucose transport, directed from the mesothelial to the interstitial side of the peritoneum, decreased slightly after the addition of gentamicin.
authors: Department of Clinical Biochemistry, Karol Marcinkowski University of Medical Sciences, Poznań, Poland.
authors: K
authors: K
authors: Czyzewska
authors: B
authors: B
authors: Szary
authors: J
authors: J
authors: Waniewski
mesh_terms: D000465:Algorithms; D000704:Analysis of Variance; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003840:Deoxycholic Acid / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology; D015314:Dialysis Solutions / Q000494:pharmacology; D004058:Diffusion; D015194:Diffusion Chambers, Culture; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D005839:Gentamicins / Q000494:pharmacology; D005936:Glucans / Q000494:pharmacology; D005947:Glucose / Q000493:pharmacokinetics* / Q000494:pharmacology; D000077607:Icodextrin; D016014:Linear Models; D008954:Models, Biological; D010537:Peritoneum / Q000187:drug effects / Q000378:metabolism*; D010539:Permeability; D011817:Rabbits
chemical_list: D000900:Anti-Bacterial Agents; D003902:Detergents; D015314:Dialysis Solutions; D005839:Gentamicins; D005936:Glucans; D003840:Deoxycholic Acid; D000077607:Icodextrin; D005947:Glucose