Butylaminiperindopril decreases transforming growth factor-beta 1 messenger RNA production in lungs of C57Bl6 mice after low-dose whole-body irradiation.
Transforming growth factor (TGF)-beta is believed to play a key role in the development of many autoimmune and malignant diseases, such as radiation and drug-induced organ disease. The aim of the present study was to determine messenger RNA (mRNA) production of TGF-beta 1 in the lungs of C57Bl6 mice after low-dose whole-body irradiation. Control (irradiated) and irradiated angiotensin-converting enzyme (ACE) inhibitor-treated animals were simultaneously examined. The ACE inhibitor group received butylaminiperindopril for 9 days after irradiation (7 Gy) at a daily dose of 0.1 mg/kg per rectum. On day 9 all mice were sacrificed and the production of mRNA TGF-beta 1 in lung tissue was determined semiquantitatively using reverse transcriptase polymerase chain reaction. In butylaminiperindopril-treated mice, a decrease in transcript of TGF-beta 1 (to 59% in comparison with controls) was observed.
Olejár
T
T
Institute of Biophysics, First Medical School, Charles University, Prague, Czech Republic.
Poucková
P
P
Zadinová
M
M
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology*; D000818:Animals; D002216:Captopril / Q000494:pharmacology; D005260:Female; D005609:Free Radicals; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D020913:Perindopril / Q000031:analogs & derivatives* / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016212:Transforming Growth Factor beta / Q000235:genetics*; D014916:Whole-Body Irradiation
D000806:Angiotensin-Converting Enzyme Inhibitors; D005609:Free Radicals; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D002216:Captopril; D020913:Perindopril