title: Characterization of an axonemal dynein heavy chain expressed early in airway epithelial ciliogenesis.
abstract: The most conspicuous evidence of airway epithelial maturation and vitality is the presence of motile cilia. In an effort to generate genetic and antigenic markers of airway maturation, injury, and repair, we characterized airway epithelial expression of a gene identified by two human expressed sequence tags that encoded peptides with sequence similarity to an invertebrate ciliary dynein heavy chain (DHC). Molecular analyses showed that the gene has a very large RNA transcript that encodes a very high molecular weight polypeptide with biochemical properties that are characteristic of a dynein heavy chain. Expression of the gene transcript correlated with the presence of ciliated cells in tissues, and immunohistochemical localization of the gene product confirmed its presence in the cilia of mature airway epithelium. In epithelium undergoing ciliogenesis ex vivo, expression of the gene transcript preceded ciliation of the epithelium and the gene product was present in the cytoplasm and at the apical border of nonciliated cells. These data suggested that the gene encodes an axonemal DHC that is expressed early during ciliogenesis, before the appearance of cilia.
authors: Departments of Pediatrics and Cell Biology and Anatomy, University of North Carolina at Chapel Hill, 27599-7310, USA.
authors: W
authors: W
authors: Reed
authors: J L
authors: JL
authors: Carson
authors: B M
authors: BM
authors: Moats-Staats
authors: T
authors: T
authors: Lucier
authors: P
authors: P
authors: Hu
authors: L
authors: L
authors: Brighton
authors: T M
authors: TM
authors: Gambling
authors: C H
authors: CH
authors: Huang
authors: M W
authors: MW
authors: Leigh
authors: A M
authors: AM
authors: Collier
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000032:analysis; D015152:Blotting, Northern; D001980:Bronchi / Q000378:metabolism; D002460:Cell Line; D002923:Cilia / Q000378:metabolism / Q000648:ultrastructure; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004398:Dyneins / Q000235:genetics* / Q000276:immunology; D004848:Epithelium / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014132:Trachea / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D018076:DNA, Complementary; D012333:RNA, Messenger; D004398:Dyneins