Electroporation-mediated gene transfer in free-swimming embryonic Xenopus laevis.
Xenopus laevis are a rich resource for vertebrate embryology and cell biology. Transplantation and transgenesis have provided much information about the developmental mechanisms of embryogenesis and molecule function, however existing methods have faced limitations regarding either the precise localization of gene expression or flexibility in the timing of gene transfer. Here we have found that electroporation of tailbud (stage 29/30) embryos is a rapid and efficient method of combining cell-specific expression with variation in temporal delivery. At the low voltages required for electroporation, embryos resumed normal swimming behavior and development. We conclude that electroporation has wide experimental application to Xenopus developmental and cell biology.
Eide
F F
FF
Department of Neurology, University of Chicago, 5841 S. Maryland Avenue, Chicago, IL 60637, USA. feide@neurology.bsd.uchicago.edu
Eisenberg
S R
SR
Sanders
T A
TA
D000818:Animals; D000229:Dependovirus / Q000235:genetics / Q000502:physiology; D004553:Electric Conductivity; D018274:Electroporation / Q000379:methods*; D004625:Embryo, Nonmammalian / Q000502:physiology*; D047108:Embryonic Development; D015870:Gene Expression; D009900:Optic Nerve / Q000378:metabolism; D009928:Organ Specificity; D011401:Promoter Regions, Genetic / Q000235:genetics; D013550:Swimming; D013997:Time Factors; D014161:Transduction, Genetic; D014162:Transfection / Q000379:methods*; D019076:Transgenes / Q000235:genetics; D014982:Xenopus laevis / Q000196:embryology* / Q000235:genetics* / Q000502:physiology / Q000821:virology