title: I-309 binds to and activates endothelial cell functions and acts as an angiogenic molecule in vivo.
abstract: Several chemokines have been shown to act as angiogenic molecules or to modulate the activity of growth factors such as fibroblast growth factor 2 (FGF-2) and vascular endothelial growth factor (VEGF). The detection of the CC chemokine receptor (CCR) 8 message in human umbilical vein endothelial cells (HUVECs) by reverse transcription- polymerase chain reaction (RT-PCR) and RNase protection assay (RPA), prompted us to investigate the potential role exerted by the CC chemokine I-309, a known ligand of such receptor, in both in vitro and in vivo angiogenesis assays. We show here that I-309 binds to endothelial cells, stimulates chemotaxis and invasion of these cells, and enhances HUVEC differentiation into capillary-like structures in an in vitro Matrigel assay. Furthermore, I-309 is an inducer of angiogenesis in vivo in both the rabbit cornea and the chick chorioallantoic membrane assay (CAM).
authors: Laboratory of Vascular Pathology, Istituto Dermopatico dell'Immacolata-Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Rome, Italy.
authors: G
authors: G
authors: Bernardini
authors: G
authors: G
authors: Spinetti
authors: D
authors: D
authors: Ribatti
authors: G
authors: G
authors: Camarda
authors: L
authors: L
authors: Morbidelli
authors: M
authors: M
authors: Ziche
authors: A
authors: A
authors: Santoni
authors: M C
authors: MC
authors: Capogrossi
authors: M
authors: M
authors: Napolitano
mesh_terms: D000482:Allantois / Q000098:blood supply / Q000187:drug effects; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects; D054402:Chemokine CCL1; D019742:Chemokines, CC / Q000378:metabolism / Q000494:pharmacology*; D002633:Chemotaxis / Q000187:drug effects; D002642:Chick Embryo; D002823:Chorion / Q000098:blood supply / Q000187:drug effects; D003094:Collagen; D003315:Cornea / Q000098:blood supply / Q000187:drug effects; D004338:Drug Combinations; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007797:Laminin; D008297:Male; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D011509:Proteoglycans; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011817:Rabbits; D054401:Receptors, CCR8; D019707:Receptors, Chemokine / Q000096:biosynthesis / Q000235:genetics; D014471:Umbilical Veins
chemical_list: C065971:CCL1 protein, human; C103330:CCR8 protein, human; D054402:Chemokine CCL1; D019742:Chemokines, CC; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; D012333:RNA, Messenger; D054401:Receptors, CCR8; D019707:Receptors, Chemokine; C059485:matrigel; D003094:Collagen