Methodological improvement of the protein phosphatase inhibition assay for the detection of okadaic acid in mussels.
A simplified procedure for the enzyme inhibition assay to measure okadaic acid and DTX-1 in mussels, based on the use of a commercially available enzyme preparation, is presented. The detection limit is 10 ng of toxin per g of digestive glands. Using Certified Reference Material (MUS-2), high accuracy and good precision is demonstrated for contamination levels higher than 32 ng g(-1). Twenty samples can be processed in about 9 h by one operator, at the cost of US$ 10 per sample. Some possibilities for further enhancing the sensitivity and reducing the processing time are discussed and a monitoring example is presented.
Della Loggia
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DEMREP, University of Trieste, Italy.
Sosa
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Tubaro
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D000818:Animals; D001681:Biological Assay / Q000191:economics; D049872:Bivalvia / Q000737:chemistry*; D004784:Environmental Monitoring / Q000379:methods; D004791:Enzyme Inhibitors / Q000032:analysis*; D051379:Mice; D019319:Okadaic Acid / Q000032:analysis* / Q000031:analogs & derivatives; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors*; D011714:Pyrans / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D004791:Enzyme Inhibitors; D011714:Pyrans; D019319:Okadaic Acid; C051904:dinophysistoxin 1; D010749:Phosphoprotein Phosphatases