Design and use of electrochemical sensors in enantioselective high throughput screening of drugs. A minireview.
The importance of reliable detection systems for enantiomeric assays increases with the necessity of high throughput screening analysis of raw materials for the pharmaceutical industry. The utilization of electrochemical sensors in enantioselective analysis is an accurate and precise alternative to chromatographic techniques. The reliability of the response characteristics as well as of the analytical information obtained by using electrochemical sensors is strictly correlated with the design of the sensors. The designs evaluated for sensors have been based on PVC, imprinting polymers and carbon paste matrices. Among these, carbon paste sensors have been the most reliable and have been utilized for the construction of potentiometric, enantioselective membrane electrodes as well as for amperometric biosensors, and immunosensors. There are two ways to use the electrochemical sensors in enantioselective screening analysis: selective binding and catalyst selectivity. A molecule with a special chemical architecture is required for selective binding: a lock for a key. The high reliability of analytical information obtained using these sensors has made possible the automation of potentiometric and amperometric techniques by integration of enantioselective sensors as detectors in flow injection analysis and sequential injection analysis techniques.
Stefana
R I
RI
Department of Chemistry, University of Pretoria, Pretoria. 0002, South Africa. Raluca.Stefan@chem.up.ac.za
van Staden
J L
JL
Aboul-Enein
H Y
HY
D000818:Animals; D015195:Drug Design; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D006801:Humans; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D013237:Stereoisomerism
D004364:Pharmaceutical Preparations