title: Immunohistochemical localization of certain inducers related to the proliferation of the pituitary gland and a study on cell contacts between the presumptive neurohypophysis and the adenohypophysis in the foetal mouse.
abstract: Optic and confocal laser scanning microscopy (CLSM) and the LsAB method were used to immunolocalize TGF alpha, EGF, FGF-2 and their related receptors, which are involved in the regulation of organogenesis in the mouse hypophysis. Internalization of the above receptors and the active proliferation of the presumptive adenohypophysis and neurohypophysis were observed during the mid-foetal stage. CLSM images were used to map the distribution of Cx32 in the proliferating hypophysis, particularly between the closely apposed neuro- and adenohypophyses. Using conventional transmission electron microscopy, gap junctions were observed at the boundary of these structures. The results suggest that cell coupling via gap junctions may provide positional information that is then used to control the differentiation of the hypophyseal cells.
authors: Department of Oral Anatomy, Osaka Dental University, Japan.
authors: Y
authors: Y
authors: Iwai-Liao
authors: S
authors: S
authors: Kumabe
mesh_terms: D000818:Animals; D002455:Cell Division; D004815:Epidermal Growth Factor / Q000378:metabolism*; D066246:ErbB Receptors / Q000378:metabolism; D005333:Fetus / Q000378:metabolism*; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D051379:Mice; D008813:Mice, Inbred ICR; D018613:Microscopy, Confocal; D010903:Pituitary Gland, Anterior / Q000166:cytology / Q000378:metabolism*; D010904:Pituitary Gland, Posterior / Q000166:cytology / Q000378:metabolism*; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism; D051497:Receptor, Fibroblast Growth Factor, Type 2; D017468:Receptors, Fibroblast Growth Factor / Q000378:metabolism; D016211:Transforming Growth Factor alpha / Q000378:metabolism*
chemical_list: D017468:Receptors, Fibroblast Growth Factor; D016211:Transforming Growth Factor alpha; D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor; D066246:ErbB Receptors; C496354:Fgfr2 protein, mouse; D020794:Receptor Protein-Tyrosine Kinases; D051497:Receptor, Fibroblast Growth Factor, Type 2