Water as polar modifier in normal phase chiral separation of 2-oxo-3-piperidineacetamide analogue.
A small amount of water present in a normal phase chiral HPLC with a Chiralcel OD column was found to be critical and necessary to separate a pharmaceutical chiral intermediate, 3-piperidineacetamide analogue. The amount of water in the normal mobile phase was optimized, and a likely mechanism of the separation was proposed.
Ning
J G
JG
Chemical Process Technologies Analytical Research, Schering Plough Research Institute, 1011 Morris Ave., Union, NJ 07083, USA. Jian.Ning@spcorp.com