Calbindin-D28k in cerebrovascular extrinsic innervation system of the rat.
Calbindin-D28k, one of the calcium-binding proteins, belongs to the EF hand family and is commonly found in neurons. It serves as a representative neuronal marker for neuroanatomical investigations. The authors' knowledge of its precise function, however, is yet very limited. In this study, we examined the existence of nerve fibers with calbindin-D28k immunoreactivity in the cerebral blood vessels and ganglia that innervate the cerebral blood vessels in the rat. Numerous nerve fibers with calbindin-D28k immunoreactivity were observed on the walls of the major extracerebral arteries forming the circle of Willis and its branches. Calbindin-D28k immunoreactivity was seen in many neurons of the trigeminal, dorsal root and jugular ganglia. A small number of neurons showed calbindin-D28k immunoreactivity in the otic and superior cervical ganglia. Calbindin-D28k immunoreactivity was not detected in the sphenopalatine or internal carotid ganglia. Pericellular basket-like formations of nerve terminals with calbindin-D28k immunoreactivity were observed in the sphenopalatine, otic, internal carotid and superior cervical ganglia. The present study demonstrated calbindin-D28k immunoreactivity in the cerebrovascular nerve fibers as well as in their origins--the cranial ganglia. These findings are significant in understanding the calcium-mediated mechanism of the neural control of the cerebral blood vessels.
Shimizu
T
T
Department of Neurology, School of Medicine, Keio University, Tokyo, Japan.
Suzuki
N
N
Takao
M
M
Koto
A
A
Fukuuchi
Y
Y
D000818:Animals; D064092:Calbindin 1; D064026:Calbindins; D002118:Calcium / Q000502:physiology; D020013:Calcium Signaling; D002536:Cerebral Arteries / Q000294:innervation*; D002941:Circle of Willis / Q000294:innervation; D005725:Ganglia, Autonomic / Q000737:chemistry*; D005726:Ganglia, Parasympathetic / Q000737:chemistry; D017950:Ganglia, Sensory / Q000737:chemistry*; D005727:Ganglia, Spinal / Q000737:chemistry; D008297:Male; D009412:Nerve Fibers / Q000737:chemistry*; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000502:physiology*; D009474:Neurons / Q000737:chemistry; D051381:Rats / Q000502:physiology*; D017207:Rats, Sprague-Dawley; D064030:S100 Calcium Binding Protein G / Q000032:analysis / Q000502:physiology*; D017783:Superior Cervical Ganglion / Q000737:chemistry; D012668:Trigeminal Ganglion / Q000737:chemistry; D014666:Vasomotor System / Q000502:physiology
C576500:Calb1 protein, rat; D064092:Calbindin 1; D064026:Calbindins; D009419:Nerve Tissue Proteins; D064030:S100 Calcium Binding Protein G; D002118:Calcium