Enantioseparation of anionic analytes by non-aqueous capillary electrophoresis using quinine and quinidine derivatives as chiral counter-ions.
A non-aqueous capillary electrophoretic method developed for the enantioseparation of N-protected amino acids has been applied to the investigation of five new quinine and quinidine derivatives as chiral selectors: 1-adamantyl carbamoylated quinine, 3,4-dichlorophenyl carbamoylated quinidine, allyl carbamoylated dihydroquinine, allyl carbamoylated dihydroquinidine and 1-methyl quininium iodide. The composition of the background electrolyte was 12.5 mM ammonia, 100 mM octanoic acid in an ethanol-methanol (60:40 v/v) mixture containing a 10 mM concentration of the chiral selector. Under these conditions, the enantioseparation of a series of various benzoyl, 3,5-dinitrobenzoyl and 3,5-dinitrobenzyloxycarbonyl amino acid derivatives was studied with respect to selectand-selector relationship and enantioselectivity.
Piett
V
V
Institute of Public Health, Louis Pasteur, Laboratory of Drug Analysis, Bruxelles, Belgium. Veronique.Piette@iph.fgov.be
Lindner
W
W
Crommen
J
J
D000838:Anions; D019075:Electrophoresis, Capillary / Q000379:methods*; D011802:Quinidine / Q000737:chemistry*; D011803:Quinine / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013237:Stereoisomerism
D000838:Anions; D011803:Quinine; D011802:Quinidine