Chromatography of human prothrombin from Nitschmann fraction III on DEAE Sepharose Fast Flow using axial and radial flow column.
An axial column (3 x 2.6 cm) and a radial flow column (3.5 x 5 cm) packed with DEAE Sepharose Fast Flow media was evaluated for the separation of human prothrombin from Nitschmann fraction III. Under radial flow conditions, a sample flow rate up to 14 mL/min (approximately 18 bed vols/h) was achieved. Breakthrough capacity was determined and both columns had almost the same breakthrough capacity per mL media, indicating that the sample loading was independent of radial column geometry.
Sun
T
T
Institute of Transfusion Medicine, PO Box 130(9), 27 TaiPing Road, Beijing 100850, People's Republic of China. sunt@cin.bmi.ac.cn
Chen
G
G
Liu
Y
Y
Bu
F
F
Wen
M
M
D002852:Chromatography, Ion Exchange / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D011516:Prothrombin / Q000302:isolation & purification*
D011516:Prothrombin