Influenza virus (A/HK/156/97) hemagglutinin expressed by an alphavirus replicon system protects chickens against lethal infection with Hong Kong-origin H5N1 viruses.
Venezuelan equine encephalitis virus replicon particles (VRP) containing the gene expressing hemagglutinin (HA) from the human Hong Kong Influenza A isolate (A/HK/156/97) were evaluated as vaccines in chicken embryos and young chicks. Expressed HA was readily detected in bird-tissue staining with anti-H5 HA antibody and in chicken cells infected with the replicon preparations following immunoprecipitation with monoclonal antibody. Birds challenged with a dose of the lethal parent virus were protected to different extents depending on the age of the bird. In ovo and 1-day-old inoculated animals that received no boost with the VRP were partially protected; birds 2 weeks of age were completely protected with a single dose of VRP.
Schultz-Cherry
S
S
SE Poultry Research Laboratory, ARS, USDA, 934 College Station Road, Athens, Georgia 30605, USA.
Dybing
J K
JK
Davis
N L
NL
Williamson
C
C
Suarez
D L
DL
Johnston
R
R
Perdue
M L
ML
D000367:Age Factors; D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D004666:Encephalitis Virus, Venezuelan Equine / Q000235:genetics*; D005822:Genetic Vectors; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D053124:Influenza A Virus, H5N1 Subtype; D009980:Influenza A virus / Q000276:immunology*; D007251:Influenza, Human / Q000517:prevention & control* / Q000662:veterinary / Q000821:virology; D011201:Poultry Diseases / Q000517:prevention & control; D012093:Replicon / Q000235:genetics*; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000008:administration & dosage*; D014765:Viral Vaccines / Q000008:administration & dosage*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D014614:Vaccines, Synthetic; D014765:Viral Vaccines