DNA extraction from coral reef sediment bacteria for the polymerase chain reaction.
A rapid and effective method for the direct extraction of high molecular weight amplifiable DNA from two coral reef sediments was developed. DNA was amplified by the polymerase chain reaction (PCR) using 16S rDNA specific primers. The amplicons were digested with HaeIII, HinP1I and MspI and separated using polyacrylamide gel electrophoresis and silver staining. The resulting amplified ribosomal DNA restriction analysis (ARDRA) patterns were used as a fingerprint to discern differences between the coral reef sediment samples. Results indicated that ARDRA is an effective method for determining differences within the bacterial community amongst different environmental samples.
Guthrie
J N
JN
Centre for Bacterial Diversity and Identification, Department of Microbiology, The University of Queensland, 4072, Brisbane, Australia. j.guthrie@cqu.edu.au
Moriarty
D J
DJ
Blackall
L L
LL
D000818:Animals; D001315:Australia; D003063:Cnidaria / Q000382:microbiology*; D016172:DNA Fingerprinting / Q000379:methods*; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004275:DNA, Ribosomal / Q000302:isolation & purification; D019015:Geologic Sediments / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal