title: Specific genetic interference with behavioral rhythms in Drosophila by expression of inverted repeats.
abstract: We describe a new experimental technique that allows for a tissue-specific reduction of gene activity in the Drosophila nervous system. On the basis of the observation that certain gene functions can be ubiquitously blocked by injecting double-stranded RNA into Drosophila embryos, we employed a method to interfere with an individual gene function permanently in a predetermined cell type. This was achieved by the formation of an inverted-repeat RNA sequence in the tissue of interest under control of the GAL4/UAS binary expression system. As an example, we show that inverted-repeat-mediated interference with the period gene produces a hypomorphic period phenotype. A selective decrease of period RNA appears to be a component of the cellular response.
authors: Laboratory of Genetics, and National Science Foundation Science and Technology Center for Biological Timing, The Rockefeller University, New York, New York 10021, USA.
authors: S
authors: S
authors: Martinek
authors: M W
authors: MW
authors: Young
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001522:Behavior, Animal; D007446:Chromosome Inversion; D002940:Circadian Rhythm / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics* / Q000502:physiology; D005656:Fungal Proteins / Q000235:genetics; D005786:Gene Expression Regulation / Q000235:genetics*; D019476:Insect Proteins / Q000235:genetics / Q000502:physiology; D008845:Microinjections; D009687:Nuclear Proteins / Q000235:genetics* / Q000502:physiology; D009928:Organ Specificity; D056950:Period Circadian Proteins; D017956:Photoreceptor Cells, Invertebrate; D011401:Promoter Regions, Genetic; D016372:RNA, Antisense / Q000494:pharmacology; D012330:RNA, Double-Stranded / Q000008:administration & dosage / Q000235:genetics / Q000494:pharmacology*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000502:physiology; D012091:Repetitive Sequences, Nucleic Acid; D012243:Rhodopsin / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D005656:Fungal Proteins; C052130:GAL4 protein, S cerevisiae; D019476:Insect Proteins; D009687:Nuclear Proteins; C539040:PER protein, Drosophila; D056950:Period Circadian Proteins; D016372:RNA, Antisense; D012330:RNA, Double-Stranded; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C087289:gl protein, Drosophila; C096445:tim protein, Drosophila; D012243:Rhodopsin