Ischaemic preconditioning ameliorates functional disturbance and impaired renal perfusion in rat ischaemia-reperfused kidneys.
1. The effects of ischaemic preconditioning (IP) on renal function, haemodynamics and lipid peroxidation in the rat ischaemia-reperfused kidney model were examined. 2. In Wistar male rats, application of a single or three periods of 5 min bilateral renal ischaemia was performed prior to 30 min bilateral ischaemia and 90 min reperfusion (IR). The glomerular filtration rate (GFR) was estimated in terms of inulin clearance. Fractional excretion of sodium (FE(Na)) and lithium (FE(Li)), indicating total and proximal tubular sodium handling, respectively, was also measured and renal blood flow was monitored throughout the experiment. In addition, renal lipid peroxidation (LPO) levels in reperfused kidneys were evaluated. 3. A 2.8-fold increase in recovery of GFR (P < 0.005), a 50% reduction in FE(Na) (P < 0.005) and a 40% decrease in FE(Li) (P < 0.05) after IR resulted from the single period of 5 min IP. Renal blood flow was also higher than that in the control group (P < 0.01). No change of LPO levels was observed. 4. We conclude that IP may have an ability to ameliorate reperfused renal function and haemodynamics with a suitable period of preconditioned ischaemia, although this effect is independent of LPO.
Ogawa
T
T
Department of Surgery, Tokyo University, Japan. e05021@h.u-tokyo.ac.jp
Mimura
Y
Y
Hiki
N
N
Kanauchi
H
H
Kaminishi
M
M
D000818:Animals; D001794:Blood Pressure; D005919:Glomerular Filtration Rate; D019194:Ischemic Preconditioning; D007668:Kidney / Q000098:blood supply* / Q000378:metabolism / Q000503:physiopathology*; D007684:Kidney Tubules / Q000503:physiopathology; D015227:Lipid Peroxidation; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012079:Renal Circulation; D015427:Reperfusion Injury / Q000378:metabolism / Q000503:physiopathology*