title: Characterization of CAF4 and CAF16 reveals a functional connection between the CCR4-NOT complex and a subset of SRB proteins of the RNA polymerase II holoenzyme.
abstract: The CCR4-NOT transcriptional regulatory complex affects transcription both positively and negatively and consists of the following two complexes: a core 1 x 10(6) dalton (1 MDa) complex consisting of CCR4, CAF1, and the five NOT proteins and a larger, less defined 1.9-MDa complex. We report here the identification of two new factors that associate with the CCR4-NOT proteins as follows: CAF4, a WD40-containing protein, and CAF16, a putative ABC ATPase. Whereas neither CAF4 nor CAF16 was part of the core CCR4-NOT complex, both CAF16 and CAF4 appeared to be present in the 1.9-MDa complex. CAF4 also displayed physical interactions with multiple CCR4-NOT components and with DBF2, a likely component of the 1.9-MDa complex. In addition, both CAF4 and CAF16 were found to interact in a CCR4-dependent manner with SRB9, a component of the SRB complex that is part of the yeast RNA polymerase II holoenzyme. The three related SRB proteins, SRB9, SRB10, and SRB11, were found to interact with and to coimmunoprecipitate DBF2, CAF4, CCR4, NOT2, and NOT1. Defects in SRB9 and SRB10 also affected processes at the ADH2 locus known to be controlled by components of the CCR4-NOT complex; an srb9 mutation was shown to reduce ADH2 derepression and either an srb9 or srb10 allele suppressed spt10-enhanced expression of ADH2. In addition, srb9 and srb10 alleles increased ADR1(c)-dependent ADH2 expression; not4 and not5 deletions are the only other known defects that elicit this phenotype. These results suggest a close physical and functional association between components of the CCR4-NOT complexes and the SRB9, -10, and -11 components of the holoenzyme.
authors: Department of Biochemistry and Molecular Biology, University of New Hampshire, Durham, New Hampshire 03824.
authors: H Y
authors: HY
authors: Liu
authors: Y C
authors: YC
authors: Chiang
authors: J
authors: J
authors: Pan
authors: J
authors: J
authors: Chen
authors: C
authors: C
authors: Salvadore
authors: D C
authors: DC
authors: Audino
authors: V
authors: V
authors: Badarinarayana
authors: V
authors: V
authors: Palaniswamy
authors: B
authors: B
authors: Anderson
authors: C L
authors: CL
authors: Denis
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D000483:Alleles; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D018797:Cell Cycle Proteins / Q000737:chemistry* / Q000378:metabolism; D002850:Chromatography, Gel; D056850:Cyclin-Dependent Kinase 8; D018844:Cyclin-Dependent Kinases / Q000378:metabolism; D016213:Cyclins / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry* / Q000378:metabolism; D005838:Genotype; D056892:Mediator Complex; D009154:Mutation; D010641:Phenotype; D010957:Plasmids / Q000378:metabolism; D011233:Precipitin Tests; D011485:Protein Binding; D011494:Protein Kinases / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases; D012319:RNA Polymerase II / Q000737:chemistry* / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism; D012260:Ribonucleases; D029701:Saccharomyces cerevisiae Proteins; D017422:Sequence Analysis, DNA; D013489:Suppression, Genetic; D014157:Transcription Factors / Q000737:chemistry* / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: C089931:CDC36 protein, S cerevisiae; C079592:CDC39 protein, S cerevisiae; C519559:Caf4 protein, S cerevisiae; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D016213:Cyclins; D005656:Fungal Proteins; D056892:Mediator Complex; D012097:Repressor Proteins; C093967:SSN2 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D011494:Protein Kinases; C093345:SSN8 protein, S cerevisiae; C085768:DBF2 protein, S cerevisiae; D017346:Protein Serine-Threonine Kinases; D056850:Cyclin-Dependent Kinase 8; D018844:Cyclin-Dependent Kinases; C498309:SSN3 protein, S cerevisiae; D012319:RNA Polymerase II; C078657:CCR4 protein, S cerevisiae; D012260:Ribonucleases; D000251:Adenosine Triphosphatases