Procedure for laryngotracheal separation in the goat: development of a new animal model for analysis of craniofacial growth.
Most animal models used to study the process of postnatal craniofacial growth require direct manipulation of the craniofacial area, a growth period, then evaluation of the area. However, the scar tissue associated with direct manipulation of the craniofacial structures can produce growth abnormalities that are unrelated to the manipulation itself. To avoid this confounding variable in the study of craniofacial growth, we developed an animal model that involves laryngotracheal separation in a young animal. Our procedure completely separates the trachea from the upper aerodigestive tract and removes the site of scar tissue formation from the region of investigation. The tracheal stomas of the goats we describe were maintained for as long as 9 months. Unlike human patients, goats with laryngotracheal separation require laryngectomy tubes to prevent life-threatening stenosis of the tracheal stoma. Here we describe the operative procedure and post-operative care required for this new animal model.
Prince
M E
ME
Department of Otolaryngology-HNS, Dalhousie University, Halifax, Nova Scotia, Canada.
Nasser
J G
JG
D000667:Ampicillin / Q000008:administration & dosage; D000818:Animals; D005145:Face / Q000502:physiology*; D005260:Female; D005839:Gentamicins / Q000008:administration & dosage; D006041:Goats / Q000601:surgery*; D007825:Laryngectomy / Q000295:instrumentation / Q000662:veterinary; D007830:Larynx / Q000502:physiology; D023421:Models, Animal; D010406:Penicillins / Q000008:administration & dosage; D012886:Skull / Q000254:growth & development*; D014132:Trachea / Q000601:surgery*; D014139:Tracheostomy / Q000662:veterinary
D005839:Gentamicins; D010406:Penicillins; D000667:Ampicillin