Antibodies against bovine herpesvirus (BHV) 5 may be differentiated from antibodies against BHV1 in a BHV1 glycoprotein E blocking ELISA.
We examined whether antibodies against bovine herpesvirus (BHV) 5 cross-react with BHV1 antigens and whether they could interfere with BHV1 eradication programmes. Six calves were experimentally infected with different doses of BHV5 strain N569; homologous antibodies were first detectable on day 11 post infection; they cross-reacted in a BHV1 virus neutralisation test, in a BHV1-glycoprotein (g)-B blocking ELISA and in a BHV1-gE ELISA, but not in a BHV1-gE blocking ELISA. This study indicates that, in ongoing BHV1 eradication programmes, based on vaccines that lack gE, BHV5 infections may not lead to false-positive serological reactions in case cattle are tested for BHV1-gE antibodies by the BHV1-gE blocking ELISA; antibodies against BHV5 may be differentiated from antibodies against BHV1. The BHV1-gE blocking ELISA may, therefore, offer opportunities for the serological differentiation between BHV1 and BHV5 infections.
Wellenberg
G J
GJ
Department of Mammalian Virology, Institute for Animal Science and Health (ID-Lelystad), P.O. Box 65, 8200 AB, Lelystad, The Netherlands. g.j.wellenberg@id.wag-ur.nl
Mars
M H
MH
Van Oirschot
J T
JT
D018138:Alphaherpesvirinae / Q000276:immunology*; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology / Q000302:isolation & purification*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006566:Herpesviridae Infections / Q000276:immunology / Q000662:veterinary; D014759:Viral Envelope Proteins / Q000276:immunology*; D014764:Viral Proteins
D000914:Antibodies, Viral; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C046998:bovine herpesvirus type-1 glycoproteins