First demonstration of an inhibitory activity of milk proteins against human immunodeficiency virus-1 reverse transcriptase and the effect of succinylation.
A variety of milk proteins including lactoferrin, angiogenin-1, alpha-lactalbumin, beta-lactoglobulin, lactoperoxidase, casein and the novel whey proteins lactogenin and glycolactin were tested for inhibitory activity toward human immunodeficiency virus-1 reverse transcriptase (HIV-1 RT), alpha-glucosidase, beta-glucosidase and beta-glucuronidase. Lactoferrin exerted the most potent inhibitory action with an IC50 of about 6 microM. Lactoperoxidase, lactogenin, angiogenin-1 and glycolactin inhibited HIV-1 RT activity with decreasing potencies. Beta-lactoglobulin, alpha-lactalbumin and casein displayed little or no inhibitory effect. Succinylation with succinic anhydride augmented the inhibitory effect of glycolactin, beta-lactoglobulin, alpha-lactalbumin, casein and human lactoferrin. The inhibitory effect of the various milk proteins on the activities of alpha-glucosidase, beta-glucosidase and beta-glucuronidase was meager. Succinylation tended to increase the alpha-glucosidase-inhibitory effect of milk proteins but neither their beta-glucosidase-inhibitory nor beta-glucuronidase-inhibitory effect was affected.
Wang
H
H
Department of Microbiology, China Agricultural University, Beijing.
Ye
X
X
Ng
T B
TB
D000818:Animals; D002364:Caseins / Q000494:pharmacology; D002417:Cattle; D005966:Glucuronidase / Q000037:antagonists & inhibitors; D065089:Glycoside Hydrolase Inhibitors; D054303:HIV Reverse Transcriptase / Q000378:metabolism*; D015497:HIV-1 / Q000201:enzymology; D006801:Humans; D007768:Lactalbumin / Q000494:pharmacology; D007781:Lactoferrin / Q000494:pharmacology; D007782:Lactoglobulins / Q000494:pharmacology; D007784:Lactoperoxidase / Q000494:pharmacology; D008892:Milk; D008894:Milk Proteins / Q000494:pharmacology*; D008895:Milk, Human; D018894:Reverse Transcriptase Inhibitors / Q000494:pharmacology*; D012259:Ribonuclease, Pancreatic / Q000494:pharmacology; D013386:Succinates; D001617:beta-Glucosidase / Q000037:antagonists & inhibitors
D002364:Caseins; D065089:Glycoside Hydrolase Inhibitors; D007782:Lactoglobulins; D008894:Milk Proteins; D018894:Reverse Transcriptase Inhibitors; D013386:Succinates; C121812:lactogenin; D007768:Lactalbumin; D007784:Lactoperoxidase; D054303:HIV Reverse Transcriptase; C046366:angiogenin; D012259:Ribonuclease, Pancreatic; D001617:beta-Glucosidase; D005966:Glucuronidase; D007781:Lactoferrin