title: Production and purification of recombinant human alpha 2C2 adrenergic receptor using Saccharomyces cerevisiae.
abstract: The objective is to generate milligram quantities of recombinant human alpha 2C2 adrenergic receptor for X-ray crystallographic studies. It has been cloned in Saccharomyces cerevisiae, and the production level is at best about 13 pmol/mg of membrane protein, as estimated by radio-ligand binding assay. The receptor is solubilized with sucrose monolaurate followed by immunoaffinity purification and reconstitution into phospholipid vesicles. The efficiency of solubilization and immuno-purification are 60% and 91%, respectively.
authors: Institute of Biotechnology, University of Helsinki, Finland.
authors: A
authors: A
authors: Kapat
authors: V P
authors: VP
authors: Jaakola
authors: H
authors: H
authors: Heimo
authors: S
authors: S
authors: Liitti
authors: P
authors: P
authors: Heikinheimo
authors: T
authors: T
authors: Glumoff
authors: A
authors: A
authors: Goldman
mesh_terms: D006801:Humans; D008967:Molecular Biology / Q000191:economics; D018341:Receptors, Adrenergic, alpha-2 / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism
chemical_list: C547774:ADRA2C protein, human; D018341:Receptors, Adrenergic, alpha-2; D011994:Recombinant Proteins