Sequence variation in the human uracil-DNA glycosylase (UNG) gene.
Spontaneous deamination of cytosine results in a premutagenic G:U mismatch that may result in a GC-->AT transition during replication. The human UNG-gene encodes the major uracil-DNA glycosylase (UDG or UNG) which releases uracil from DNA, thus, initiating base excision repair to restore the correct DNA sequence. Bacterial and yeast mutants lacking the homologous UDG exhibit elevated spontaneous mutation frequencies. Hence, mutations in the human UNG gene could presumably result in a mutator phenotype. We screened all seven exons including exon-intron boundaries, both promoters, and one intron of the UNG gene and identified considerable sequence variation in cell lines derived from normal fibroblasts and tumour tissue. None of the sequence variants was accompanied by significantly reduced UDG activity. In the UNG gene from 62 sources, we identified 12 different variant alleles, with allele frequencies ranging from 0.01 to 0.23. We identified one variant allele per 3.8kb in non-coding regions, but none in the coding region of the gene. In promoter B we identified four different variants. A substitution within an AP2 element was observed in tumour cell lines only and had an allele frequency of 0.10. Introduction of this substitution into chimaeric promoter-luciferase constructs affected transcription from the promoter. UDG-activity varied little in fibroblasts, but widely between tumour cell lines. This variation did not however correlate with the presence of any of the variant alleles. In conclusion, mutations affecting the function of human UNG gene are seemingly infrequent in human tumour cell lines.
Kvaløy
K
K
Institute for Cancer Research and Molecular Biology, Faculty of Medicine, Norwegian University of Science and Technology, N-7005, Trondheim, Norway.
Nilsen
H
H
Steinsbekk
K S
KS
Nedal
A
A
Monterotti
B
B
Akbari
M
M
Krokan
H E
HE
D002478:Cells, Cultured; D045647:DNA Glycosylases; D005091:Exons / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D014644:Genetic Variation; D006801:Humans; D007438:Introns / Q000235:genetics; D008297:Male; D016297:Mutagenesis, Site-Directed; D009699:N-Glycosyl Hydrolases / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D014407:Tumor Cells, Cultured; D051981:Uracil-DNA Glycosidase
D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D051981:Uracil-DNA Glycosidase