title: Interaction of the influenza virus nucleoprotein with the cellular CRM1-mediated nuclear export pathway.
abstract: Influenza virus transcription occurs in the nuclei of infected cells, where the viral genomic RNAs are complexed with a nucleoprotein (NP) to form ribonucleoprotein (RNP) structures. Prior to assembly into progeny virions, these RNPs exit the nucleus and accumulate in the cytoplasm. The mechanisms responsible for RNP export are only partially understood but have been proposed to involve the viral M1 and NS2 polypeptides. We found that the drug leptomycin B (LMB), which specifically inactivates the cellular CRM1 polypeptide, caused nuclear retention of NP in virus-infected cells, indicating a role for the CRM1 nuclear export pathway in RNP egress. However, no alteration was seen in the cellular distribution of M1 or NS2, even in the case of a mutant virus which synthesizes greatly reduced amounts of NS2. Furthermore, NP was distributed throughout the nuclei of infected cells at early times postinfection but, when retained in the nucleus at late times by LMB treatment, was redistributed to the periphery of the nucleoplasm. No such change was seen in the nuclear distribution of M1 or NS2 after drug treatment. Similar to the behavior of NP, M1 and NS2 in infected cells, LMB treatment of cells expressing each polypeptide in isolation caused nuclear retention of NP but not M1 or NS2. Conversely, overexpression of CRM1 caused increased cytoplasmic accumulation of NP but had little effect on M1 or NS2 distribution. Consistent with this, NP bound CRM1 in vitro. Overall, these data raise the possibility that RNP export is mediated by a direct interaction between NP and the cellular CRM1 export pathway.
authors: Division of Virology, Department of Pathology, University of Cambridge, Cambridge CB2 1QP, United Kingdom.
authors: D
authors: D
authors: Elton
authors: M
authors: M
authors: Simpson-Holley
authors: K
authors: K
authors: Archer
authors: L
authors: L
authors: Medcalf
authors: R
authors: R
authors: Hallam
authors: J
authors: J
authors: McCauley
authors: P
authors: P
authors: Digard
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000502:physiology*; D002467:Cell Nucleus / Q000378:metabolism*; D002642:Chick Embryo; D006224:Cricetinae; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology; D028884:Karyopherins; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D018160:Receptors, Cytoplasmic and Nuclear; D014758:Viral Core Proteins / Q000378:metabolism*; D014763:Viral Matrix Proteins / Q000378:metabolism; D017361:Viral Nonstructural Proteins / Q000378:metabolism; D000097606:Exportin 1 Protein
chemical_list: D002352:Carrier Proteins; D005231:Fatty Acids, Unsaturated; D028884:Karyopherins; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D018160:Receptors, Cytoplasmic and Nuclear; D014758:Viral Core Proteins; D014763:Viral Matrix Proteins; D017361:Viral Nonstructural Proteins; D000097606:Exportin 1 Protein; C031540:M-protein, influenza virus; C488936:M1 protein, Influenza A virus; C038753:leptomycin B