Automated detection of morphological alterations during apoptosis.
A convenient means of measuring apoptosis is described using an automated analyzer (MF-1; Sysmex), which normally provides rapid measurement of malarial parasites. By means of this MF-1 procedure, apoptotic cells exhibited characteristic changes of light scatter (size) and fluorescence (DNA content) relating to cell shrinkage and nuclear fragmentation of apoptosis.
Lertworasirikul
T
T
Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Bunyaratvej
A
A
Kubota
F
F
D017209:Apoptosis / Q000502:physiology*; D001331:Automation / Q000295:instrumentation / Q000379:methods; D002467:Cell Nucleus / Q000648:ultrastructure*; D048429:Cell Size; D002470:Cell Survival; D004247:DNA / Q000032:analysis; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods; D018922:HL-60 Cells / Q000166:cytology; D006801:Humans; D008027:Light; D012542:Scattering, Radiation
D004247:DNA