Flexor tendon blood vessels.
The aim of this study was to assess rabbit long flexor tendon vascularity in a qualitative and quantitative manner using immunohistochemistry. The endothelial cell surface marker CD31 was targeted with a specific monoclonal mouse-anti-human antibody with good species cross-reactivity. Subsequent signal amplification and chromogen labelling allowed vessel visualization. Computer image analysis was performed. Values for vessel number and total vessel area per section, as well as the sections' cross-sectional tendon areas, were obtained. There was a consistent deep tendon avascular zone between the A2 and A4 pulley in the rabbit forepaw. This was not the case in the hindpaw, with dorsally orientated longitudinal vessels coursing the length of the intrasynovial tendon. The area of least vascularity in the hindpaw was around the metacarpophalangeal joint. We therefore recommend the use of hindpaw tendons when using the rabbit as a flexor tendon experimental model. This is because its vascular pattern is similar to that of the human flexor digitorum profundus.
Jones
M E
ME
RAFT Institute, Mount Vernon Hospital Northwood, Middlesex, UK.
Ladhani
K
K
Mudera
V
V
Grobbelaar
A O
AO
McGrouther
D A
DA
Sanders
R
R
D000818:Animals; D000911:Antibodies, Monoclonal; D005552:Forelimb; D006614:Hindlimb; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D011817:Rabbits; D013710:Tendons / Q000098:blood supply*; D014034:Toes
D000911:Antibodies, Monoclonal