Expression of protein kinase C isozymes in nonpregnant and pregnant human myometrium.
OBJECTIVE
The aim of this study was to compare the distributions of protein kinase C isozymes in human nonpregnant and pregnant myometrial tissues and primary cell cultures.


METHODS
Myometrial tissues were obtained at hysterectomy from nonpregnant women and at cesarean delivery from women both before and during early labor at term. Western immunoblot analysis was performed on homogenates of myometrial tissues and primary cell cultures with monoclonal antibodies specific for protein kinase C isozymes. Redistribution and translocation of protein kinase C were examined by means of immunocytochemical methods.


RESULTS
Nonpregnant myometrial tissues contained protein kinase C isozymes alpha, gamma, delta, mu, iota, and zeta but not beta(1), beta(2), theta, or epsilon. Pregnant myometrial tissues both before and during early labor contained the same protein kinase C isozymes and also beta(1) and beta(2). The protein kinase C isozyme distribution in primary myometrial cell cultures was identical to that in the myometrial tissues. Protein kinase C redistribution and translocation were demonstrated in these cultured myometrial cells.


CONCLUSIONS
Both human myometrial tissues and primary cell cultures expressed a broad range of protein kinase C isozymes. Protein kinase C isozymes beta(1) and beta(2) were absent in nonpregnant myometrium but were induced during pregnancy. Labor at term did not alter protein kinase C isozyme expression.
Hurd
W W
WW
Department of Obstetrics and Gynecology and the Department of Internal Medicine, Indiana University School of Medicine, Indianapolis, USA.
Fomin
V P
VP
Natarajan
V
V
Brown
H L
HL
Bigsby
R M
RM
Singh
D M
DM
D001692:Biological Transport / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000378:metabolism*; D007743:Labor, Obstetric / Q000378:metabolism; D009215:Myometrium / Q000166:cytology / Q000378:metabolism*; D010121:Oxytocin / Q000494:pharmacology; D011247:Pregnancy / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D012016:Reference Values; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014018:Tissue Distribution / Q000187:drug effects
D007527:Isoenzymes; D010121:Oxytocin; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate