Identical marker alleles in Podolic cattle (Bos taurus) and Indian zebu (Bos indicus).
In the context of biochemical marker research and in order to add new information on native breeds, the present work focuses on a local Southern Italy cattle, namely Italian Podolic. We provide the complete structural characterisation of alpha-lactalbumins and beta-globin chains isolated from Podolic cattle (Bos taurus). Given the unavailability of the complete sequence for alpha-lactalbumin A of taurine cattle in the literature, we intended to check its structure in order to ascertain the absence of any possible silent mutation. Screening the Podolic cattle, we found a new beta-globin variant not detectable by conventional methods. The presence of such a new variant might be helpful in the study of the Podolic population genetic structure and for a better knowledge of the gene pool per se, and in comparison with the other breeds. Structural analyses showed that the new beta-globin Podolic variant exhibited the same sequence as beta-globin Azebu. The alpha-lactalbumin A was the same as that isolated from zebu cattle (Bos indicus). The results are discussed in relation to the possible involvement of the two markers in the debate on the origin of the Podolic breed.
Pieragostini
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Department Progettazione e Gestione dei Sistemi agro-zootecnico-forestali, Università degli Studi, Bari, Italy.
Scaloni
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A
Rullo
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R
Di Luccia
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A
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle / Q000235:genetics*; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005914:Globins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D007525:Isoelectric Focusing; D007768:Lactalbumin / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D013058:Mass Spectrometry; D008892:Milk / Q000737:chemistry; D008894:Milk Proteins / Q000737:chemistry / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D011110:Polymorphism, Genetic; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D013997:Time Factors; D000067816:Whey Proteins
D008894:Milk Proteins; D000067816:Whey Proteins; D005914:Globins; D007768:Lactalbumin