RNA-Catalyzed CoA, NAD, and FAD synthesis from phosphopantetheine, NMN, and FMN.
A novel in vitro selection method was developed to isolate RNA sequences with coenzyme-synthesizing activities. We used size-heterogeneous libraries containing randomized ribonucleotide sequences of four different lengths (30N, 60N, 100N, and 140N), all with 5'-ATP initiation. Two RNAs, CoES7 (30N) and CoES21 (60N), are able to catalyze the synthesis of three common coenzymes, CoA, NAD, and FAD, from their precursors, 4'-phosphopantetheine, NMN, and FMN, respectively. Both ribozymes require divalent manganese for activities. The results support the availability of these coenzymes in an RNA world, and point to a chemical explanation for the complex bipartite structures of many coenzymes.
Huang
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Department of Chemistry and Biochemistry, University of Southern Mississippi, Hattiesburg, Mississippi 39406-5043, USA. faqing.h.huang@usm.edu
Bugg
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CW
Yarus
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M
D003065:Coenzyme A / Q000235:genetics* / Q000378:metabolism; D005486:Flavin Mononucleotide / Q000378:metabolism; D005182:Flavin-Adenine Dinucleotide / Q000096:biosynthesis* / Q000235:genetics*; D015723:Gene Library; D009243:NAD / Q000235:genetics* / Q000378:metabolism; D009537:Nicotinamide Mononucleotide / Q000378:metabolism; D010204:Pantetheine / Q000031:analogs & derivatives / Q000378:metabolism; D012313:RNA / Q000235:genetics* / Q000302:isolation & purification; D017423:Sequence Analysis, RNA
D009243:NAD; D009537:Nicotinamide Mononucleotide; D005182:Flavin-Adenine Dinucleotide; D010204:Pantetheine; D012313:RNA; D005486:Flavin Mononucleotide; C003129:4'-phosphopantetheine; D003065:Coenzyme A