Utilisation of differential killer toxin sensitivity patterns for fingerprinting and clustering yeast strains belonging to different genera.
The differential killer sensitivity of 103 yeast cultures belonging to 12 species (genera Debaryomyces, Kluyveromyces, Saccharomyces, and Zygosaccharomyces), all previously taxonomically certified by nDNA-nDNA reassociation, against a given panel of 39 killer yeasts was used as a fingerprinting tool. All strains, with the only exception of eight cultures belonging to the species Zygosaccharomyces bailii, were characterised by a specific, individual sensitivity pattern (killer formula). Cluster analysis of binary sequences based on killer sensitivity of strains belonging to different genera is presented and discussed.
Buzzini
P
P
Dipartimento di Biologia Vegetale e Biotecnologie Agroambientali, Sezione di Microbiologia Applicata, University of Perugia, Italy. pbuzzini@unipg.it
Martini
A
A
D016000:Cluster Analysis; D005656:Fungal Proteins / Q000494:pharmacology; D055646:Killer Factors, Yeast; D007716:Kluyveromyces / Q000145:classification / Q000235:genetics; D008826:Microbial Sensitivity Tests; D016533:Mycological Typing Techniques / Q000379:methods*; D009183:Mycotoxins / Q000494:pharmacology*; D012440:Saccharomyces / Q000145:classification / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D004718:Saccharomycetales / Q000145:classification* / Q000235:genetics; D020068:Zygosaccharomyces / Q000145:classification / Q000235:genetics
D005656:Fungal Proteins; C527157:KHR1 protein, S cerevisiae; D055646:Killer Factors, Yeast; D009183:Mycotoxins; D029701:Saccharomyces cerevisiae Proteins