[Neutralizing antibodies to 5 HIV-1 strains in macaques immunized with the multi-epitope polypeptide TAB9].
Presence of neutralizing antibodies(Nab) to 5 laboratory strains of human immunodeficiency virus type-1 (HIV-1) was studied and compared in the sera of eight macaca (Macaca fascicularis) after the third and fourth immunizations with multi-epitope polypeptide TAB9, emulsified with adjuvant Montanide ISA 720. Four animals were inoculated 1 mg and the others 200 micrograms, 2 animals were used as controls and injected only with adjuvant. Although the presence of neutralizing antibodies to homologous strains of HIV-group group B was confirmed in most animals after immunization through antigen p24 capture ELISA (DAVIH-Agp24, Cuba), no statistically significant differences were found neither in titers caused by antigen concentrations nor in the responses after the third and fourth immunizing doses. Controls did not develop neutralizing antibodies.
Navea Leyva
L M
LM
Centro de Investigaciones Científicas de la Defensa Civil, San José de las Lajas, La Habana, Cuba.
Lobaina Bartelemy
L
L
Dubed Echevarría
M
M
Alvarez Seguí
G
G
Gómez Rodríguez
C
C
Duarte Cano
C
C
D000704:Analysis of Variance; D000818:Animals; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes / Q000008:administration & dosage / Q000276:immunology*; D015483:HIV Antibodies / Q000097:blood*; D015488:HIV Antigens / Q000008:administration & dosage / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D007114:Immunization / Q000379:methods; D008252:Macaca fascicularis; D009500:Neutralization Tests / Q000379:methods / Q000706:statistics & numerical data; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000276:immunology*
D000939:Epitopes; D015483:HIV Antibodies; D015488:HIV Antigens; D011993:Recombinant Fusion Proteins; C417682:TAB9