[The application of competitive RT-PCR to detect minimal residual disease in patients with 8;21 translocation].
OBJECTIVE
To construct and evaluate the competitive quantitative RT-PCR method for detecting the fusion gene of AML1-ETO in the patients of t(8;21)AML(acute myeloid leukemia).


METHODS
The authors used the method of splicing by overlapping extension (SOE) to obtain the competitive DNA fragment, with which they set up the competitive quantitative RT-PCR assay to detect AML1-ETO chimeric genes in the patients of t(8;21) AML.


RESULTS
The competitive DNA fragment has been obtained; the sensitive competitive RT-PCR method is developed, which allows the quantitation of the number of AML1-ETO transcriptions in t(8;21)AML patients at different phases.


CONCLUSIONS
The competitive quantitative RT-PCR method, based on SOE, is simple and convenient; t(8;21) AML patients in different survival conditions express AML1-ETO gene quite differently.
Wang
Y
Y
Jiangsu Institute of Haematology, the First Affiliated Hospital of Suzhou Medical College, Suzhou, Jiangsu, 215006 P.R.China.
Seng
M
M
Guo
Y
Y
Lu
D
D
Chen
Z
Z
Xue
Y
Y
D002891:Chromosomes, Human, Pair 21; D002898:Chromosomes, Human, Pair 8; D050676:Core Binding Factor Alpha 2 Subunit; D004268:DNA-Binding Proteins / Q000235:genetics; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D018365:Neoplasm, Residual; D011518:Proto-Oncogene Proteins; D000075142:RUNX1 Translocation Partner 1 Protein; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics; D014178:Translocation, Genetic
D050676:Core Binding Factor Alpha 2 Subunit; D004268:DNA-Binding Proteins; D011518:Proto-Oncogene Proteins; D000075142:RUNX1 Translocation Partner 1 Protein; C493728:RUNX1 protein, human; C082222:RUNX1T1 protein, human; D014157:Transcription Factors