[Evaluation of cytotoxic activity of Vibrio cholerae non-01 culture filtrate on established cell lines and human diploid cells].
The cell-destroying effect of cell free filtrates of 90 V. cholerae non-01 cultures was measured by titration method in 3 established cell lines: CHO, HeLa and Vero and in 3 human diploid cells cultures: MRC-5, WI-38 and PZ. The vibrio strains differed in the titre of toxic effect. Most sensitive was CHO cell line, least sensitive were human diploid cell cultures. It was found that bacterial strains produced different substances toxic for various cell lines. Among them NAG-ST toxin produced by 41% of examined strains was identified and hemolysins/cytolysins activity was evaluated. Both may play a role in the pathogenicity of those strains for humans.
Pancer
K
K
Zakład Bakteriologii PZH w Warszawie.
Stypułkowska-Misiurewicz
H
H
Jarzabek
Z
Z
D000818:Animals; D016466:CHO Cells / Q000187:drug effects; D002474:Cell-Free System; D002478:Cells, Cultured / Q000187:drug effects; D002522:Chlorocebus aethiops; D006224:Cricetinae; D004768:Enterotoxins / Q000633:toxicity*; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D012703:Serotyping; D013045:Species Specificity; D017186:Titrimetry; D014709:Vero Cells / Q000187:drug effects; D014734:Vibrio cholerae / Q000472:pathogenicity*
D004768:Enterotoxins; C037420:stN protein, Vibrio cholerae