title: Transgenic rainbow trout expressed sGnRH-antisense RNA under the control of sGnRH promoter of Atlantic salmon.
abstract: A recombinant vector containing antisense DNA complementary to Atlantic salmon (Salmo salar) sGnRH cDNA driven by specific promoter Pab derived from a corresponding sGnRH gene was introduced into rainbow trout (Oncorhynchus mykiss) eggs. This resulted in transgenic animals that had integrated one copy of the transgene into their genome and transmitted it through the germline. Antisense-sGnRH mRNA (AS) was expressed mainly in the brain of transgenic AS(+) fish. Levels of sGnRH endogenous mRNA in the brain were lower in 11-month-old AS(+) fish compared with nontransgenic AS(-) individuals from the same F2 progeny. sGnRH levels significantly decreased in the pituitary of transgenic males and females around the maturation period and in the brain of AS(+) immature females compared with controls. No reliable statistical difference was found in the levels of FSH and LH between AS(+) and AS(-) groups either in immature or mature fish. The majority of transgenic fish reached maturity at the same time as did nontransgenic individuals, although the maturation of AS(+) animals seemed to be more asynchronous. For the first time, the influence of antisense messengers on endogenous mRNA in transgenic fish and the corresponding protein is described.
authors: Station Commune de Recherches en Ichtyophysiologie, Biodiversité et Environment INRA, Campus de Beaulieu, 35042 Rennes, France.
authors: S
authors: S
authors: Uzbekova
authors: J
authors: J
authors: Chyb
authors: F
authors: F
authors: Ferrière
authors: T
authors: T
authors: Bailhache
authors: P
authors: P
authors: Prunet
authors: P
authors: P
authors: Alestrom
authors: B
authors: B
authors: Breton
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D001921:Brain / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D015870:Gene Expression; D007987:Gonadotropin-Releasing Hormone / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D006066:Gonads / Q000254:growth & development; D047908:Intracellular Signaling Peptides and Proteins; D007763:Lac Operon; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D016376:Oligonucleotides, Antisense / Q000235:genetics*; D017686:Oncorhynchus mykiss / Q000235:genetics*; D010902:Pituitary Gland / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000502:physiology*; D012313:RNA / Q000235:genetics*; D012474:Salmon / Q000235:genetics*; D019076:Transgenes / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D016376:Oligonucleotides, Antisense; C086961:PAB protein, Peptostreptococcus magnus; D007987:Gonadotropin-Releasing Hormone; D012313:RNA; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone