Evaluation of random amplified polymorphic DNA typing of Pseudomonas aeruginosa.
A method for distinguishing among Pseudomonas aeruginosa strains using random amplified polymorphic DNA (RAPD) typing was evaluated for reproducibility and discriminatory power. A total of 200 isolates, blinded in triplicate, were evaluated by RAPD. All 600 samples were typeable; 197 of 200 isolates gave identical results on all three occasions, and 131 distinct RAPD types were identified.
Campbell
M
M
Division of Infectious and Immunological Diseases, Department of Pediatrics, University of British Columbia, and Children's and Women's Health Centre of British Columbia, Vancouver, British Columbia V5Z 4H4, Canada.
Mahenthiralingam
E
E
Speert
D P
DP
D015373:Bacterial Typing Techniques; D003550:Cystic Fibrosis / Q000382:microbiology; D006801:Humans; D011550:Pseudomonas aeruginosa / Q000145:classification* / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique