Identification of the homozygously deleted region at chromosome 1p36.2 in human neuroblastoma.
BACKGROUND
We have identified for the first time a homozygously deleted region within the smallest region of overlap at 1p36.2-3 in two neuroblastoma cell lines.


METHODS
The 800-kb PAC contig covering the entire homozygously deleted region was made and sequenced. To date, approximately 70% of sequencing has been accomplished, and the estimated length of the deleted region was 500 kb.


RESULTS
Currently, we have found six genes within the region, which include three known genes as well as three other genes that have been reported during processing of our present project for the last 3(1/2) years. We report here the results of expression and mutation analyses of those genes.


CONCLUSIONS
Full sequencing for the region of homozygous deletion as well as further analyses of the genes mapped within the region may reveal whether or not there is a neuroblastoma suppressor gene as proposed by the Knudson's two-hit hypothesis.
Nakagawara
A
A
Division of Biochemistry, Chiba Cancer Center Research Institute, Chiba, Japan. akiranak@chiba-cc.pref.chiba.jp
Ohira
M
M
Kageyama
H
H
Mihara
M
M
Furuta
S
S
Machida
T
T
Takayasu
H
H
Islam
A
A
Nakamura
Y
Y
Takahashi
M
M
Shishikura
T
T
Kaneko
Y
Y
Toyoda
A
A
Hattori
M
M
Sakaki
Y
Y
Ohki
M
M
Horii
A
A
Soeda
E
E
Inazawa
J
J
Seki
N
N
Kuma
H
H
Nozawa
I
I
Sakiyama
S
S
D002675:Child, Preschool; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics*; D015870:Gene Expression; D006720:Homozygote; D006801:Humans; D019656:Loss of Heterozygosity; D009447:Neuroblastoma / Q000235:genetics*; D014407:Tumor Cells, Cultured