Application of a laser scalpel to sectioning fragile tissues prior to optical sectioning microscopy and 3D reconstruction.
A method is described for the cutting of fragile material with a laser scalpel which minimizes damage to friable materials, making the interior of structures accessible for optical sectioning microscopy or for high resolution X-ray microtomography followed by 3D reconstruction.
Cookson
M J
MJ
Department of Nursing and Paramedic Sciences, Hillside House, University of Hertfordshire, College Lane, Hatfield, Herts., AL10 9AB, UK.
Entwistle
A
A
D006801:Humans; D007834:Lasers; D008168:Lung / Q000033:anatomy & histology*; D008867:Microtomy / Q000379:methods*; D014056:Tomography, X-Ray