High-density fiber-optic DNA random microsphere array.
A high-density fiber-optic DNA microarray sensor was developed to monitor multiple DNA sequences in parallel. Microarrays were prepared by randomly distributing DNA probe-functionalized 3.1-microm-diameter microspheres in an array of wells etched in a 500-microm-diameter optical imaging fiber. Registration of the microspheres was performed using an optical encoding scheme and a custom-built imaging system. Hybridization was visualized using fluorescent-labeled DNA targets with a detection limit of 10 fM. Hybridization times of seconds are required for nanomolar target concentrations, and analysis is performed in minutes.
Ferguson
J A
JA
Department of Chemistry, Tufts University, Medford, Massachusetts 02155, USA.
Steemers
F J
FJ
Walt
D R
DR
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D005336:Fiber Optic Technology; D008863:Microspheres; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D055100:Optical Fibers; D012680:Sensitivity and Specificity
D015345:Oligonucleotide Probes; D004247:DNA