Cell-mediated immune responses to autologous virus in HIV-1-seropositive individuals after treatment with an HIV-1 immunogen.
OBJECTIVE
We hypothesized that cell mediated immune responses to an HIV-1 immunogen (whole-killed, gp120-depleted HIV-1 in IFA, REMUNE) would include those to autologous virus.


METHODS
Five chronically HIV-1 infected individuals were examined for HIV-specific immune responses to their own virus (autologous viral antigen) after treatment with an HIV-1 immunogen.


RESULTS
Subjects had low proliferative responses to HIV and p24 antigens prior to immunization and mounted strong lymphocyte proliferative responses to the immunizing HIV-1 virus, native p24, and autologous viral antigen post immunization. Similarly, subjects produced low amounts of interferon-gamma in response to HIV and p24 antigens prior to immunization and increased their interferon-gamma production in response to HIV-1, native p24, and to autologous antigen post-immunization. Furthermore, beta-chemokine responses measured as migratory inhibitory protein-1beta production were low at baseline in response to HIV-1 and native p24 antigens and were enhanced post immunization to HIV-1, native p24, and autologous antigen.


CONCLUSIONS
In this study HIV-specific immune responses to autologous virus were observed after treatment with an HIV-specific immunogen.
Moss
R B
RB
The Immune Response Corporation, Carlsbad, California, USA.
Giermakowska
W K
WK
Wallace
M R
MR
Savary
J R
JR
Jensen
F C
FC
Carlo
D J
DJ
D016915:AIDS Vaccines / Q000008:administration & dosage / Q000276:immunology; D054407:Chemokine CCL4; D016655:HIV Core Protein p24 / Q000276:immunology* / Q000627:therapeutic use*; D015658:HIV Infections / Q000276:immunology; D015497:HIV-1 / Q000276:immunology; D006801:Humans; D007114:Immunization; D007371:Interferon-gamma / Q000096:biosynthesis; D008213:Lymphocyte Activation; D019402:Macrophage Inflammatory Proteins / Q000096:biosynthesis
D016915:AIDS Vaccines; D054407:Chemokine CCL4; D016655:HIV Core Protein p24; D019402:Macrophage Inflammatory Proteins; C105086:remune; D007371:Interferon-gamma