title: Myeloma proteins that bind Hsp65 (GroEL) are polyreactive and are found in high incidence in pristine induced plasmacytomas.
abstract: The myeloma proteins produced by 44 plasmacytomas (PCTs) recently induced by pristane in BALB/cAnPt and closely related PCT susceptible congenic strains of mice were isolated chromatographically and screened against a panel of 10 protein, nucleic acid and lipid antigens. This sample was highly unusual because 82% of the proteins had IgG isotopes. Nine of the proteins bound to Hsp65 (GroEL), and all of these were polyreactative. Twenty-one of the myeloma proteins were polyreactive and bound two or more antigens in the panel, and five were monoreactive. Thus, an antigen binding activity was determined for 59% of these myeloma proteins.
authors: Laboratory of Genetics, National Cancer Institute, National Institute of Health, Bethesda, MD 20892 USA
authors: M
authors: M
authors: Potter
authors: G
authors: G
authors: Jones
authors: W
authors: W
authors: Dubois
authors: K
authors: K
authors: Williams
authors: E
authors: E
authors: Mushinski
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D001168:Arthritis / Q000139:chemically induced / Q000276:immunology; D001202:Ascitic Fluid / Q000737:chemistry; D001426:Bacterial Proteins; D018834:Chaperonin 60 / Q000276:immunology* / Q000378:metabolism; D018833:Chaperonins / Q000276:immunology* / Q000378:metabolism; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D007143:Immunoglobulin Heavy Chains / Q000235:genetics / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009194:Myeloma Proteins / Q000276:immunology* / Q000378:metabolism; D010954:Plasmacytoma / Q000139:chemically induced / Q000276:immunology*; D013729:Terpenes / Q000633:toxicity*
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60; D007143:Immunoglobulin Heavy Chains; D009194:Myeloma Proteins; D013729:Terpenes; C074119:heat-shock protein 65, Mycobacterium; C009042:pristane; D018833:Chaperonins