title: The tumour-suppressor genes lgl and dlg regulate basal protein targeting in Drosophila neuroblasts.
abstract: Drosophila neuroblasts are a model system for studying asymmetric cell division: they divide unequally to produce an apical neuroblast and a basal ganglion mother cell that differ in size, mitotic activity and developmental potential. During neuroblast mitosis, an apical protein complex orients the mitotic spindle and targets determinants of cell fate to the basal cortex, but the mechanism of each process is unknown. Here we show that the tumour-suppressor genes lethal giant larvae (lgl) and discs large (dlg) regulate basal protein targeting, but not apical complex formation or spindle orientation, in both embryonic and larval neuroblasts. Dlg protein is apically enriched and is required for maintaining cortical localization of Lgl protein. Basal protein targeting requires microfilament and myosin function, yet the lgl phenotype is strongly suppressed by reducing levels of myosin II. We conclude that Dlg and Lgl promote, and myosin II inhibits, actomyosin-dependent basal protein targeting in neuroblasts.
authors: Howard Hughes Medical Institute, University of Oregon, Eugene 97403, USA.
authors: C Y
authors: CY
authors: Peng
authors: L
authors: L
authors: Manning
authors: R
authors: R
authors: Albertson
authors: C Q
authors: CQ
authors: Doe
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D016764:Cell Polarity; D003931:Diacetyl / Q000031:analogs & derivatives* / Q000494:pharmacology; D004330:Drosophila; D029721:Drosophila Proteins; D017344:Genes, Insect; D016147:Genes, Tumor Suppressor; D019476:Insect Proteins / Q000235:genetics* / Q000502:physiology; D008938:Mitosis; D008969:Molecular Sequence Data; D009218:Myosins / Q000037:antagonists & inhibitors / Q000502:physiology; D009474:Neurons / Q000166:cytology*; D021381:Protein Transport; D025521:Tumor Suppressor Proteins
chemical_list: D029721:Drosophila Proteins; D019476:Insect Proteins; D025521:Tumor Suppressor Proteins; C081435:l(2)gl protein, Drosophila; C070350:dlg1 protein, Drosophila; C004717:diacetylmonoxime; D009218:Myosins; D003931:Diacetyl