Choice of insufflating gas influences on wound metastasis.
BACKGROUND
Laparoscopic cancer surgery is limited by concerns about port-site metastasis. No study has definitively addressed the behavior and growth of tumor cells after the use of specific laparoscopic gases.


METHODS
In athymic rats, 10,000 colon cancer cells were injected intraperitoneally. The rats received either no pneumoperitoneum (pneumo) or pneumo (8 mmHg, 10 min) with carbon dioxide (CO(2)), nitrous oxide (N(2)O), or air. Two full-thickness incisions were made and closed in the upper abdomen of each animal. After 4 weeks, implants were identified grossly at necropsy, and invasiveness was scored according to penetration through the layers of the abdominal wall.


RESULTS
Rats receiving pneumo had more frequent implants (p < 0.01) with deeper penetration (p < 0.001) than rats not receiving pneumo. Implants were more common after air pneumo than after CO(2) (p < 0.05) or N(2)O (p = 0.07) pneumo, and were less penetrating after CO(2) pneumo than after air (p < 0.001) or N(2)O (p < 0.05) pneumo.


CONCLUSIONS
Carbon dioxide gas may limit the viability and invasiveness of free intraperitoneal tumor cells, as compared with air or N(2)O.
Farrell
T M
TM
Department of Surgery, University of North Carolina at Chapel Hill, Campus Box 7210, Burnett Womack Clinical Sciences Building, Chapel Hill, NC 27599-7210, USA.
Metreveli
R E
RE
Johnson
A B
AB
Smith
C D
CD
Hunter
J G
JG
D000009:Abdominal Muscles / Q000473:pathology; D000008:Abdominal Neoplasms / Q000453:epidemiology / Q000473:pathology / Q000556:secondary*; D000388:Air; D000818:Animals; D002245:Carbon Dioxide; D003110:Colonic Neoplasms; D015994:Incidence; D009361:Neoplasm Invasiveness; D009366:Neoplasm Seeding; D009368:Neoplasm Transplantation; D009609:Nitrous Oxide; D011028:Pneumoperitoneum, Artificial / Q000379:methods*; D051381:Rats; D011923:Rats, Nude
D002245:Carbon Dioxide; D009609:Nitrous Oxide