Transposon-based approaches to identify essential bacterial genes.
Transposons are a powerful tool for identifying genes essential for bacterial viability. The availability of many bacterial genome sequences and the large number of genes of unknown function therein have inspired the generation of a variety of different approaches. These methods are described and their advantages and disadvantages are discussed.
Judson
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Dept of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA.
Mekalanos
J J
JJ
D001419:Bacteria / Q000235:genetics* / Q000254:growth & development; D004251:DNA Transposable Elements; D005798:Genes, Bacterial; D020043:Genes, Essential; D016680:Genome, Bacterial; D016254:Mutagenesis, Insertional
D004251:DNA Transposable Elements