Regional expression of protein phosphatase type 1 and 2A catalytic subunit isoforms in the human heart.
In mammalian species, including man, the duration of myocardial contraction is shorter in atria than ventricles. Total contraction time depends at least in part on phosphorylation and dephosphorylation of cardiac regulatory proteins. Dephosphorylation reactions are mediated by protein phosphatases. In the mammalian heart more than 90% of the protein phosphatase (PP) activity consists of PP1 and PP2A. Therefore, the aim of this study was to investigate which isoforms of PP1 and PP2A are present in human myocardium and whether their expression is regionally different. RT-PCR and Northern blotting revealed that all isoforms of PP1 and PP2A presently known are expressed in the human heart. Expression levels of PP1 alpha, delta, and gamma as well as 2A alpha were higher in right ventricles than in right atria. However, there was no such difference for PP2A beta. At the protein level PP1 alpha was unchanged, whereas PP2A was by 56% higher in right ventricles compared to atria. The phosphorylation state of TnI was lower in right ventricle than in right atrium. Thus, lower protein expression of PP2A in atrium could contribute to the faster relaxation by increasing the phosphorylation state of TnI. We conclude that expression of PP1 and PP2A isoforms is regionally regulated in the human heart.
Lüss
H
H
Institut für Pharmakologie und Toxikologie, Westfälische Wilhelms-Universität Münster, Germany. luss@uni-muenster.de
Klein-Wiele
O
O
Bokník
P
P
Herzig
S
S
Knapp
J
J
Linck
B
B
Müller
F U
FU
Scheld
H H
HH
Schmid
C
C
Schmitz
W
W
Neumann
J
J
D015152:Blotting, Northern; D015153:Blotting, Western; D002384:Catalysis; D006325:Heart Atria / Q000378:metabolism; D006352:Heart Ventricles / Q000378:metabolism; D006801:Humans; D009206:Myocardium / Q000378:metabolism*; D010749:Phosphoprotein Phosphatases / Q000096:biosynthesis* / Q000737:chemistry; D010766:Phosphorylation; D020033:Protein Isoforms; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014018:Tissue Distribution; D019210:Troponin I / Q000096:biosynthesis
D020033:Protein Isoforms; D019210:Troponin I; D010749:Phosphoprotein Phosphatases