Relation between phosphatidylserine exposure and store-operated Ca(2+) entry in stimulated cells.
A significant increase in intracellular Ca(2+) is required to trigger the remodeling of the cell plasma membrane. Scott syndrome is an extremely rare inherited disorder of the transmembrane migration of phosphatidylserine toward the exoplasmic leaflet in blood cells. We have recently reported a reduced capacitative Ca(2+) entry in Scott cells [Martínez et al. (1999) Biochemistry 38, 10092-10098]. We have investigated here the links between defective phosphatidylserine exposure and Ca(2+) signaling in Scott cells by focusing on the Ca(2+) entry following the emptying of intracellular stores. After depletion of caffeine- or thapsigargin-sensitive stores, Ca(2+) entry was lower in Scott compared to control lymphoblasts. However, the simultaneous depletion of both types of stores restored a normal Ca(2+) influx across the plasma membrane in Scott cells and phosphatidylserine externalization ability was improved concomitantly with capacitative Ca(2+) entry. These observations point to the essential role of capacitative Ca(2+) entry in the control of phosphatidylserine exposure of stimulated cells.
Martin
S
S
Institut d'Hématologie et d'Immunologie, Faculté de Médecine, 4, rue Kirschleger, Strasbourg, 67085, France.
Laude-Lemaire
I
I
Kerbiriou-Nabias
D
D
Freyssinet
J M
JM
Martínez
M C
MC
D000368:Aged; D001402:B-Lymphocytes / Q000187:drug effects / Q000502:physiology*; D001778:Blood Coagulation Disorders / Q000097:blood* / Q000235:genetics; D002110:Caffeine / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000097:blood*; D020013:Calcium Signaling / Q000187:drug effects / Q000502:physiology*; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D004533:Egtazic Acid / Q000494:pharmacology; D005260:Female; D006470:Hemorrhage / Q000209:etiology; D004854:Herpesvirus 4, Human / Q000502:physiology; D006801:Humans; D010718:Phosphatidylserines / Q000097:blood*; D012016:Reference Values; D013577:Syndrome; D019284:Thapsigargin / Q000494:pharmacology
D010718:Phosphatidylserines; D000001:Calcimycin; D002110:Caffeine; D004533:Egtazic Acid; D019284:Thapsigargin; D002118:Calcium