Prevention of proteolysis in cold-stored rat liver by addition of amino acids to the preservation solution.
BACKGROUND
One process identified as detrimental in liver preservation is proteolysis.


METHODS
We tested the effects of adding antiproteolytic amino acids (L-alanine, L-glutamine, L-histidine, L-leucine, L-methionine, L-phenylalanine, L-proline, L-tryptophan) to the preservation medium, in a model of reperfusion of 24 h cold-stored rat livers.


RESULTS
During the preservation period, antiproteolytic amino acids inhibited the proteolysis observed in stored livers as shown by branched-chain amino acid fluxes, which switched from release to uptake. During reperfusion, cold storage of lives without the addition of antiproteolytic amino acids resulted in a decrease in the total amino acid and branched-chain amino acid uptake and a lower perfusion flow rate. The addition of antiproteolytic amino acids during liver storage resulted in the maintenance of total amino acid and branched-chain amino acid uptake and a significant improvement in the perfusion flow rate during reperfusion.


CONCLUSIONS
The presence of antiproteolytic amino acids in the preservation medium might be of interest in improving hepatic graft viability in transplantation.
Charrueau
C
C
*Laboratoire de Pharmacie Galénique, Faculté des Sciences Pharmaceutiques et Biologiques de l'Université René Descartes, Paris, France. charruea@pharmacie.univ-paris5.fr
Blondé-Cynober
F
F
Coudray-Lucas
C
C
Poupon
R
R
Chaumeil
J C
JC
Hannoun
L
L
Cynober
L
L
D000596:Amino Acids / Q000008:administration & dosage* / Q000494:pharmacology; D000818:Animals; D003080:Cold Temperature; D003627:Data Interpretation, Statistical; D006868:Hydrolysis; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D016031:Liver Transplantation; D008297:Male; D008954:Models, Biological; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D010477:Perfusion; D011506:Proteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015424:Reperfusion; D013997:Time Factors
D000596:Amino Acids; D019188:Organ Preservation Solutions; D011506:Proteins