The quality of a liver graft.
Because transplantation success is influenced by the quality of the graft, the objective of this study was to find parameters to evaluate transplant livers in the recipient centre. In 64 liver grafts, the venous effluates of a portal back-table flush were investigated for various parameters. Amongst them, glutathione S-transferase (GST), glutamate dehydrogenase (GLDH) and the leucocyte count were found superior in predicting graft survival. Using the combination of these parameters, 100-day graft survival of between 95% (all parameters positive) and 0% (all parameters negative) was predicted. We concluded that good liver grafts are characterized by a low width of injury (cytosolic component: GST), a low depth of injury (mitochondrial component: GLDH), as well as by a potential to induce tolerance (passenger leucocytes). Perfusate analysis seems to be a valuable tool to recognize problematic grafts in advance and to quantify the "graft factor" in considerations concerning quality control.
Schenk
M
M
Department of General Surgery, University of Tübingen, Germany.
Zipfel
A
A
Sauer
U
U
Lauchart
W
W
Viebahn
R
R
D000241:Adenosine; D000493:Allopurinol; D000704:Analysis of Variance; D004798:Enzymes / Q000097:blood; D005260:Female; D005978:Glutathione; D006085:Graft Survival; D006801:Humans; D007328:Insulin; D007958:Leukocyte Count; D008099:Liver; D008111:Liver Function Tests; D016031:Liver Transplantation / Q000502:physiology*; D008297:Male; D009926:Organ Preservation / Q000379:methods; D019188:Organ Preservation Solutions; D010477:Perfusion; D011237:Predictive Value of Tests; D011887:Raffinose; D012189:Retrospective Studies; D013997:Time Factors
D004798:Enzymes; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose