title: Influence of ampicillin, ceftiofur, attenuated live PRRSV vaccine, and reduced dose Streptococcus suis exposure on disease associated with PRRSV and S. suis coinfection.
abstract: The objective of this research was to evaluate the efficacy of two antimicrobials (ampicillin and ceftiofur), a modified-live porcine reproductive and respiratory syndrome virus (PRRSV) vaccine, and low dose exposure to Streptococcus suis on disease associated with PRRSV/S. suis coinfection. Fifty-six, crossbred, PRRSV-free pigs were weaned at 10-12 days of age and randomly assigned to five treatment groups. All pigs were inoculated with 2ml of 10(6.4) TCID50/ml of high virulence PRRSV isolate VR-2385 intranasally at 29-31 days of age (day 0 of the study) followed 7 days later by intranasal inoculation with 2ml of 10(8.9)colony forming units(CFU)/ml S. suis type 2 isolate ISU VDL #40634/94. Pigs in group 1 (n=10) served as untreated infected positive controls. Pigs in group 2 (n=12) were treated with 5.0 mg/kg ceftiofur hydrochloride intramuscularly (IM) on days 8, 11, and 14. Pigs in group 3 (n=11) were treated with 11 mg/kg ampicillin IM on days 8-10. Pigs in group 4 (n=12) were vaccinated 14 days prior to PRRSV challenge with a commercial modified-live PRRSV vaccine. Pigs in group 5 (n=11) were exposed to a 1:100 dilution of the S. suis challenge inoculum 19 days prior to S. suis challenge. Mortality was 80, 25, 82, 83, and 36% in groups 1-5, respectively. The reduced dose S. suis exposure had some residual virulence, evidenced by S. suis induced meningitis in two pigs after exposure. Treatment with ceftiofur hydrochloride and reduced dose exposure to S. suis were the only treatments which significantly (P<0.05) reduced mortality associated with PRRSV/S. suis coinfection, significantly (P<0.05) reduced recovery of S. suis from tissues at necropsy, and significantly (P<0.05) reduced the severity of gross lung lesions.
authors: Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.
authors: C S
authors: CS
authors: Schmitt
authors: P G
authors: PG
authors: Halbur
authors: J A
authors: JA
authors: Roth
authors: J M
authors: JM
authors: Kinyon
authors: C
authors: C
authors: Kasorndorkbua
authors: B
authors: B
authors: Thacker
mesh_terms: D000667:Ampicillin / Q000627:therapeutic use*; D000818:Animals; D002511:Cephalosporins / Q000627:therapeutic use*; D003131:Combined Modality Therapy / Q000662:veterinary; D008168:Lung / Q000473:pathology; D010406:Penicillins / Q000627:therapeutic use*; D019318:Porcine Reproductive and Respiratory Syndrome / Q000382:microbiology* / Q000473:pathology / Q000628:therapy; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology*; D013290:Streptococcal Infections / Q000150:complications / Q000473:pathology / Q000662:veterinary*; D017011:Streptococcus suis / Q000276:immunology*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology* / Q000473:pathology / Q000628:therapy; D014765:Viral Vaccines / Q000494:pharmacology*
chemical_list: D002511:Cephalosporins; D010406:Penicillins; D014765:Viral Vaccines; D000667:Ampicillin; C053503:ceftiofur