Dietary conjugated linoleic acid did not alter immune status in young healthy women.
The purpose of this study was to examine whether conjugated linoleic acid (CLA) supplementation in human diets would enhance indices of immune status as reported by others for animal models. Seventeen women, 20-41 yr, participated in a 93-d study conducted in two cohorts of 9 and 8 women at the Metabolic Research Unit of Western Human Nutrition Research Center. Seven subjects were fed the basal diet (19, 30, and 51% energy from protein, fat, and carbohydrate, respectively) throughout the study. The remaining 10 subjects were fed the basal diet for the first 30 d, followed by 3.9 g CLA (Tonalin)/d for the next 63 d. CLA made up 65% of the fatty acids in the Tonalin capsules, with the following isomeric composition: t10, c12, 22.6%; c11, t13, 23.6%; c9, t11, 17.6%; t8, c10, 16.6%; and other isomers 19.6%. Most indices of immune response were tested at weekly intervals, three times at the end of each period (stabilization/intervention); delayed-type hypersensitivity (DTH) to a panel of six recall antigens was tested on study day 30 and 90; all subjects were immunized on study day 65 with an influenza vaccine, and antibody titers were examined in the sera collected on day 65 and 92. None of the indices of immune status tested (number of circulating white blood cells, granulocytes, monocytes, lymphocytes, and their subsets, lymphocytes proliferation in response to phytohemagglutinin, and influenza vaccine, serum influenza antibody titers, and DTH response) were altered during the study in either dietary group. Thus, in contrast to the reports with animal models, CLA feeding to young healthy women did not alter any of the indices of immune status tested. These data suggest that short-term CLA supplementation in healthy volunteers is safe, but it does not have any added benefit to their immune status.
Kelley
D S
DS
U.S. Department of Agriculture, University of California, Department of Nutrition, Davis 95616, USA. dkelley@whnrc.usda.gov
Taylor
P C
PC
Rudolph
I L
IL
Benito
P
P
Nelson
G J
GJ
Mackey
B E
BE
Erickson
K L
KL
D000328:Adult; D000906:Antibodies / Q000097:blood; D001835:Body Weight; D015331:Cohort Studies; D004032:Diet; D004305:Dose-Response Relationship, Drug; D005227:Fatty Acids / Q000737:chemistry / Q000494:pharmacology; D005260:Female; D006801:Humans; D006967:Hypersensitivity; D007107:Immune System / Q000187:drug effects*; D007252:Influenza Vaccines / Q000494:pharmacology; D007962:Leukocytes / Q000187:drug effects; D019787:Linoleic Acid / Q000097:blood / Q000737:chemistry* / Q000494:pharmacology*; D008214:Lymphocytes / Q000187:drug effects; D010919:Placebos; D011897:Random Allocation; D013997:Time Factors
D000906:Antibodies; D005227:Fatty Acids; D007252:Influenza Vaccines; D010919:Placebos; D019787:Linoleic Acid