title: [Isolation and characteristics of anti-adenovirus monoclonal antibodies in immunoenzyme and immunofluorescent reactions].
abstract: New monoclonal antibodies (MAbs) to adenovirus hexon, highly active in ELISA and immunofluorescent analysis, were prepared. According to competitive ELISA, new MAbs differed in their blocking activity and were directed to 2 different hexon epitopes. MAb 3H8 did not modify antigen binding of the rest MAbs labeled with peroxidase (PAb x Pox), and none of unlabeled MAbs suppressed the reaction of MAb x Pox 3H8. MAbs 1E8 7F1, 1E11, and 3B1 reacted with each other but differed by the spectrum and level of competitive inhibition, which indicated that they were directed to different epitopes of adenovirus hexon. Comparison of the specific activity of MAbs 7F1 and 1E8 in direct immunofluorescent detection of adenovirus antigens in infected cell cultures and clinical materials from patients showed a good coincidence (90-97%) of the results with the IMAGEN Adenovirus test (Dako) and with polyclonal FITC conjugates to adenovirus hexon.
authors: A O
authors: AO
authors: Monaenkov
authors: V K
authors: VK
authors: Sologub
authors: A A
authors: AA
authors: Sominina
authors: I V
authors: IV
authors: Amosova
authors: N V
authors: NV
authors: Lipina
authors: K K
authors: KK
authors: Mil'kint
authors: E A
authors: EA
authors: Zibina
authors: A K
authors: AK
authors: Golovanova
authors: L B
authors: LB
authors: Potapenko
authors: L G
authors: LG
authors: Karpovich
authors: I N
authors: IN
authors: Indikova
authors: T V
authors: TV
authors: Mamontova
mesh_terms: D000256:Adenoviridae / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000914:Antibodies, Viral / Q000276:immunology*; D000918:Antibody Specificity; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D051379:Mice
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral