A marker assisted selection protocol (MASP) to generate C57BL/6J or 129S6/SvEvTac speed congenic or consomic strains.
A marker assisted selection protocol is presented that allows for the generation of congenic or consomic strains derived from a C57BL/6J:129S6/SvEvTac mixed strain background. The protocol uses defined primer pairs to generate amplicons that can be distinguished by non-denaturing agarose electrophoresis. Use of this application should result in substantial savings in time, effort, and cost for investigators in all areas of transgenic mouse research.
Estill
S J
SJ
Department of Biochemistry and Division of Molecular Cardiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390-8573, USA.
Garcia
J A
JA
D000818:Animals; D020296:Animals, Congenic / Q000235:genetics*; D005260:Female; D005819:Genetic Markers; D051379:Mice; D008810:Mice, Inbred C57BL
D005819:Genetic Markers