title: Ultrastructural organization of amyloid fibrils by atomic force microscopy.
abstract: Atomic force microscopy has been employed to investigate the structural organization of amyloid fibrils produced in vitro from three very different polypeptide sequences. The systems investigated are a 10-residue peptide derived from the sequence of transthyretin, the 90-residue SH3 domain of bovine phosphatidylinositol-3'-kinase, and human wild-type lysozyme, a 130-residue protein containing four disulfide bridges. The results demonstrate distinct similarities between the structures formed by the different classes of fibrils despite the contrasting nature of the polypeptide species involved. SH3 and lysozyme fibrils consist typically of four protofilaments, exhibiting a left-handed twist along the fibril axis. The substructure of TTR(10-19) fibrils is not resolved by atomic force microscopy and their uniform appearance is suggestive of a regular self-association of very thin filaments. We propose that the exact number and orientation of protofilaments within amyloid fibrils is dictated by packing of the regions of the polypeptide chains that are not directly involved in formation of the cross-beta core of the fibrils. The results obtained for these proteins, none of which is directly associated with any human disease, are closely similar to those of disease-related amyloid fibrils, supporting the concept that amyloid is a generic structure of polypeptide chains. The detailed architecture of an individual fibril, however, depends on the manner in which the protofilaments assemble into the fibrillar structure, which in turn is dependent on the sequence of the polypeptide and the conditions under which the fibril is formed.
authors: Oxford Centre for Molecular Sciences, New Chemistry Laboratory, Oxford OX1 3QT, United Kingdom.
authors: A K
authors: AK
authors: Chamberlain
authors: C E
authors: CE
authors: MacPhee
authors: J
authors: J
authors: Zurdo
authors: L A
authors: LA
authors: Morozova-Roche
authors: H A
authors: HA
authors: Hill
authors: C M
authors: CM
authors: Dobson
authors: J J
authors: JJ
authors: Davis
mesh_terms: D000682:Amyloid / Q000737:chemistry / Q000648:ultrastructure*; D000818:Animals; D002417:Cattle; D004220:Disulfides / Q000737:chemistry; D006801:Humans; D018625:Microscopy, Atomic Force / Q000379:methods; D008854:Microscopy, Electron; D009113:Muramidase / Q000737:chemistry / Q000648:ultrastructure; D010446:Peptide Fragments / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D019869:Phosphatidylinositol 3-Kinases / Q000737:chemistry / Q000648:ultrastructure; D011228:Prealbumin / Q000737:chemistry / Q000648:ultrastructure*; D018909:src Homology Domains
chemical_list: D000682:Amyloid; D004220:Disulfides; D010446:Peptide Fragments; D010455:Peptides; D011228:Prealbumin; D019869:Phosphatidylinositol 3-Kinases; D009113:Muramidase