Tissue distribution and timing of appearance of polytropic envelope recombinants during infection with SL3-3 murine leukemia virus or its weakly pathogenic SL3DeltaMyb5 mutant.
A time course analysis was performed to identify the sites of formation and timing of appearance of polytropic recombinant viruses following infection of NIH/Swiss mice with the murine retrovirus SL3-3 murine leukemia virus (SL3) or with a weakly pathogenic mutant termed SL3DeltaMyb5. The results indicated that (i) polytropic recombinant viruses occur initially in the thymus of SL3-infected animals, (ii) the timing of appearance of polytropic recombinants in bone marrow is not consistent with their participation in the previously reported formation of transplantable tumor-forming cells at 3 to 4 week postinoculation, and (iii) the efficient generation of recombinant virus is correlated with efficient tumor induction.
Rulli
K
K
Department of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, Louisiana 70112, USA.
Lobelle-Rich
P A
PA
Trubetskoy
A
A
Lenz
J
J
Levy
L S
LS
D000818:Animals; D001853:Bone Marrow / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D009052:Leukemia Virus, Murine / Q000302:isolation & purification*; D007942:Leukemia, Experimental / Q000821:virology*; D051379:Mice; D011995:Recombination, Genetic; D012192:Retroviridae Infections / Q000821:virology*; D013154:Spleen / Q000821:virology; D013997:Time Factors; D014412:Tumor Virus Infections / Q000821:virology*
D004279:DNA, Viral