title: Polyphenolics enhance red blood cell resistance to oxidative stress: in vitro and in vivo.
abstract: In this study we investigated the potential antioxidant properties of blueberry polyphenolics in vitro and vivo, using red blood cell (RBC) resistance to reactive oxygen species (ROS) as the model. In vitro incubation with anthocyanins or hydroxycinnamic acids (HCA) (0.5 and 0.05 mg/ml) was found to enhance significantly RBC resistance to H2O2 (100 microM) induced ROS production. This protection was also observed in vivo following oral supplementation to rats at 100 mg/ml. However, only anthocyanins were found to afford protection at a significant level, this at 6 and 24 h post supplementation. This protection was not consistent with the measured plasma levels of anthocyanins. Indeed, plasma polyphenolic concentrations were highest after 1 h, declining considerably after 6 h and not detected after 24 h. The difference in absorption between anthocyanins and HCA is likely to have contributed to the observed difference in their abilities to afford protection to RBC. This protection represents a positive role following dietary consumption of polyphenolics from blueberries, against ROS formation within RBC in vivo.
authors: Jean Mayer Human Nutrition Research Center on Aging at Tufts University, United States Department of Agriculture, Boston, MA 02111, USA. kyoudim@hnrc.tufts.edu
authors: K A
authors: KA
authors: Youdim
authors: B
authors: B
authors: Shukitt-Hale
authors: S
authors: S
authors: MacKinnon
authors: W
authors: W
authors: Kalt
authors: J A
authors: JA
authors: Joseph
mesh_terms: D000818:Animals; D000872:Anthocyanins / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects / Q000502:physiology*; D005419:Flavonoids; D005638:Fruit; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology*; D010666:Phenylpropionates / Q000494:pharmacology; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D011916:Rats, Inbred F344; D017382:Reactive Oxygen Species / Q000502:physiology
chemical_list: D000872:Anthocyanins; D005419:Flavonoids; D010636:Phenols; D010666:Phenylpropionates; D011108:Polymers; D017382:Reactive Oxygen Species; C035253:3-phenylpropionic acid; D006861:Hydrogen Peroxide