title: Effect of n-3 fatty acid deficiency on fatty acid composition and metabolism of aminophospholipids in rat brain synaptosomes.
abstract: Docosahexaenoic acid (DHA, 22:6n-3) is one of the major polyunsaturated fatty acids esterified predominantly in aminophospholipids such as ethanolamine glycerophospholipid (EtnGpl) and serine glycerophospholipid (SerGpl) in the brain. Synaptosomes prepared from rats fed an n-3 fatty acid-deficient safflower oil (Saf) diet had significantly decreased 22:6n-3 content with a compensatory increased 22:5n-6 content when compared with rats fed an n-3 fatty acid-sufficient perilla oil (Per) diet. When the Saf group was shifted to a diet supplemented with safflower oil plus 22:6n-3 (Saf + DHA) after weaning, 22:6n-3 content was found to be restored to the level of the Per group. The uptake of [3H]ethanolamine and its conversion to [3H]EtnGpl did not differ significantly among the three dietary groups, whereas the formation of [3H]lysoEtnGpl from [3H]ethanolamine was significantly lower in the Saf group than in the other groups. The uptake of [3H]serine, its incorporation into [3H]SerGpl, and the conversion into [3H]EtnGpl by decarboxylation of [3H]SerGpl did not differ among the three dietary groups. The observed decrease in lysoEtnGpl formation associated with a reduction of 22:6n-3 content in rat brain synaptosomes by n-3 fatty acid deprivation may provide a clue to reveal biochemical bases for the dietary fatty acids-behavior link.
authors: Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Nagoya, Japan. aikemoto@phar.nagoya-cu.ac.jp
authors: A
authors: A
authors: Ikemoto
authors: M
authors: M
authors: Ohishi
authors: N
authors: N
authors: Hata
authors: Y
authors: Y
authors: Misawa
authors: Y
authors: Y
authors: Fujii
authors: H
authors: H
authors: Okuyama
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D004041:Dietary Fats / Q000493:pharmacokinetics; D004281:Docosahexaenoic Acids / Q000378:metabolism; D019856:Ethanolamine / Q000493:pharmacokinetics; D015525:Fatty Acids, Omega-3 / Q000378:metabolism* / Q000502:physiology*; D005260:Female; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012450:Safflower Oil / Q000494:pharmacology; D012694:Serine / Q000493:pharmacokinetics; D013574:Synaptosomes / Q000378:metabolism*; D013997:Time Factors
chemical_list: D004041:Dietary Fats; D015525:Fatty Acids, Omega-3; D004281:Docosahexaenoic Acids; D012694:Serine; D019856:Ethanolamine; D012450:Safflower Oil