Observation of Na(+)-bound oligomers of quercetin in the gas phase.
While developing a liquid chromatography/tandem mass spectrometry method for the analysis of the flavonoid quercitin, it was observed that quercetin (3,3',4',5,7-pentahydroxyflavone) exhibited clustering in both the positive and negative ion mode. Two series of positive ion clusters were observed; the first series corresponds to singly charged [2M + Na](+) at m/z 627.2 to [13M + Na](+) at m/z 3947.5, while the second series corresponds to doubly charged [7M + 2Na](2+) at m/z 1080.4 to [25M + 2Na](2+) at m/z 3798.5. In the negative ion mode, the behavior of quercetin parallels that of apigenin (4',5,7-trihydroxyflavone) in that [M + NO(3)](-), [2M + NO(3)](-), and [3M + NO(3)](-) were observed at m/z 364.1, 666.0, and 968.9, respectively; in addition, quercitin clusters with chloride ions ([2M + Cl](-) at m/z 638.9 and [3M + Cl](-) at m/z 940. 9) were observed. The results of tandem mass spectrometric examination of several cluster ions are reported.
Croley
T R
TR
Water Quality Centre, Trent University, Peterborough ON K9J 7B8, Canada.
Hughes
R J
RJ
Hao
C
C
Metcalfe
C D
CD
March
R E
RE
D005740:Gases; D007477:Ions; D046911:Macromolecular Substances; D013058:Mass Spectrometry / Q000379:methods; D011794:Quercetin / Q000032:analysis / Q000737:chemistry*; D012964:Sodium / Q000737:chemistry*
D005740:Gases; D007477:Ions; D046911:Macromolecular Substances; D011794:Quercetin; D012964:Sodium