A novel auxiliary partial orthotopic liver transplantation model in rats.
BACKGROUND
Although auxiliary partial orthotopic liver transplantation (APOLT) has become a well-accepted procedure recently, a practical experiment model in APOLT using small animals has yet to be developed.


METHODS
Male Lewis rats were used for both donors and recipients. An auxiliary partial graft was obtained by ex vivo resection of the donor right and caudate lobes, and was transplanted orthotopically into the recipient after resection of the recipient medial and left hepatic lobes. Portal vein and hepatic duct reconstructions were by the cuff technique, and supra- and intrahepatic vena cava were sutured continuously. Operative outcomes, serum chemistry, liver tissue blood flow, angiographic and histopathological findings were then examined. Conventional orthotopic liver transplantation (OLT) procedures were also undertaken as a control.


RESULTS
One-day, 1-week and 1-month survival rate of APOLT group was 100, 85 and 85%, respectively. AST in the APOLT group on the 1st postoperative day was significantly higher than in the OLT group. No significant differences were recognized in serum albumin and total bilirubin levels between the two groups. Although the portogram of an APOLT rat showed slight narrowing at the cuff anastomosis site, both the graft and the native liver were opacified similarly. The liver tissue blood flow on the 5th postoperative day in the native liver and the graft returned to as high as 95 and 74% of the values on laparotomy, respectively. Histological examinations of the auxiliary graft 1 month after transplantation showed mild ductular proliferation and mononuclear cell infiltration around the portal triads.


CONCLUSIONS
This novel APOLT model in rats allows practical and reproducible results, and may be of value in the basic study of APOLT procedures.
Ikegami
T
T
Department of Surgery II, Kyushu University, Fukuoka, Japan. tikegami@surg2.med.kyushu-u.ac.jp
Nishizaki
T
T
Yanaga
K
K
Ohta
R
R
Hiroshige
S
S
Sugimachi
K
K
D000792:Angiography; D000818:Animals; D017078:Laser-Doppler Flowmetry; D008099:Liver / Q000503:physiopathology; D008102:Liver Circulation; D008111:Liver Function Tests; D016031:Liver Transplantation; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D016019:Survival Analysis