DNA cleavage by homo- and heterotetranuclear Cu(II) and Mn(II) complexes with tetrathioether-tetrathiol moiety.
Novel homotetranuclear Cu(II) and heteronuclear Cu(II)-Mn(II) complexes with tetrathioether-tetrathiol moiety have been prepared and their DNA relaxation activities with plasmid pCYTEXP (5kb) were electrophoretically established. The cleavage products analyzed by neutral agarose gel electrophoresis indicated that the interaction of the metal complexes with supercoiled plasmid DNA yielded linear, nicked or degraded DNA. The relaxation activities of both homo- and heterotetranuclear (SK4) complexes are time- and concentration-dependent. The findings suggest that SK4 with potent nucleolytic activity is a good nuclease substitute in the presence ofcooxidant. Furthermore, the observation of induction of DNA into smaller fragments by SK4 is also significant.
Dülger
S
S
Karadeniz Technical University, Department of Biology, Trabzon, Turkey.
Saglam
N
N
Beldüz
A O
AO
Güner
S
S
Karaböcek
S
S
D003300:Copper / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry*; D004278:DNA, Superhelical / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D008024:Ligands; D008345:Manganese / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry*; D010618:Phenanthrolines / Q000737:chemistry; D010795:Phthalic Acids / Q000737:chemistry; D010957:Plasmids / Q000737:chemistry; D013440:Sulfides / Q000737:chemistry*
D004277:DNA, Single-Stranded; D004278:DNA, Superhelical; D008024:Ligands; D009942:Organometallic Compounds; D010618:Phenanthrolines; D010795:Phthalic Acids; D013440:Sulfides; C014741:bis(1,10-phenanthroline)copper(2+) ion; D008345:Manganese; D003300:Copper; C044731:magnesium monoperoxyphthalate