Mapping of the CYP1A1, SSTR1 and TTF1 genes to pig chromosome 7q refines the porcine-human comparative map.
The cytochrome P-450, subfamily I, polypeptide 1 (CYP1A1), somatostatin receptor 1 (SSTR1) and transcription termination factor 1 (TTF1) genes, predicted to reside on SSC7q based on chromosome painting studies, were selected for genetic mapping in the pig. Porcine sequence tagged sites (STSs) spanning 0.9-1.5 kb genomic DNA sequences were generated and all genes were physically assigned to SSC7q12-q23, q26 using a pig-rodent somatic cell hybrid panel. Direct sequencing of PCR products from four different breeds revealed single nucleotide polymorphisms (SNPs) in all genes. SNPs in the SSTR1 and CYP1A1 genes allowed simple genotyping using PCR-restriction fragment length polymorphism (RFLP) analysis and linkage mapping with the PiGMaP reference families. SSTR1 was found to be closely linked to CYP1A1 (0.0 cM; LOD = 11.1) and ANPEP (0.0 cM; LOD = 9.0). This refined the localization of a previously identified breakpoint in the linkage map between regions with orthology to human chromosome 15q (represented by CYP1A1 and ANPEP) and 14q (represented by SSTR1). Moreover, multipoint linkage data suggest inverted segments of orthology between HSA14q11-q13 and SSC7q. The TTF1 SNP was confirmed by allele-specific PCR but was not informative in any of the families used for linkage analysis.
Marklund
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Department of Animal Science, Iowa State University, Ames 50011, USA.
Tuggle
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Rothschild
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D000818:Animals; D002874:Chromosome Mapping; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D008040:Genetic Linkage; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017481:Receptors, Somatostatin / Q000235:genetics*; D013552:Swine / Q000235:genetics*; D014157:Transcription Factors
D004268:DNA-Binding Proteins; D005819:Genetic Markers; D017481:Receptors, Somatostatin; C504003:TTF1 protein, human; D014157:Transcription Factors; C101130:somatostatin receptor type 1; D019363:Cytochrome P-450 CYP1A1