Analysis of neural stem cells by flow cytometry: cellular differentiation modifies patterns of MHC expression.
Neural stem cells are currently considered very hopeful candidates for cell replacement therapy in neurodegenerative pathologies such as Parkinson's disease. Here we show that different cell types derived from neurospheres amplified in vitro can be identified by FACS analysis relying solely on physical parameters (FSC/SSC) or autofluorescence. Additionally, after treatment with a panel of inflammatory cytokines, neurospheres and their differentiated progeny were shown to express MHC antigens which could potentially cause transplant rejection. Astrocytes expressed the highest levels of MHC. Hence removing such cells prior to transplantation could potentially optimise transplant survival.
McLaren
F H
FH
Laboratory of Functional Immunogenetics, Molecular Immunology Programme, Babraham Institute, Babraham, CB2 4AT, Cambridge, UK.
Svendsen
C N
CN
Van der Meide
P
P
Joly
E
E
D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D005434:Flow Cytometry; D015395:Histocompatibility Antigens Class I / Q000032:analysis*; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D007372:Interferons / Q000494:pharmacology; D009474:Neurons / Q000502:physiology*; D051381:Rats; D011917:Rats, Inbred Lew; D013234:Stem Cells / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D014409:Tumor Necrosis Factor-alpha; D007372:Interferons