Molecular characterization of a Cryptosporidium isolate from a black bear.
To further validate the observation of the existence of host-adapted strains of Cryptosporidium parvum, we genetically characterized an isolate of Cryptosporidium parasite from a black bear. Sequence analysis of the ribosomal RNA small subunit and the 70-kDa heat shock protein (HSP70) showed that this parasite represents a new genotype of C. parvum and is related to the C. parvum dog genotype. This finding is helpful for clarifying Cryptosporidium taxonomy.
Xiao
L
L
Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30341, USA.
Limor
J R
JR
Sulaiman
I M
IM
Duncan
R B
RB
Lal
A A
AA
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003457:Cryptosporidiosis / Q000469:parasitology / Q000662:veterinary*; D016785:Cryptosporidium parvum / Q000145:classification* / Q000235:genetics*; D004285:Dogs; D020459:Genes, rRNA / Q000235:genetics; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012335:RNA, Ribosomal / Q000235:genetics; D017422:Sequence Analysis, DNA; D001503:Ursidae / Q000469:parasitology*
D018840:HSP70 Heat-Shock Proteins; D012335:RNA, Ribosomal