title: Archaeal adaptation to higher temperatures revealed by genomic sequence of Thermoplasma volcanium.
abstract: The complete genomic sequence of the archaeon Thermoplasma volcanium, possessing optimum growth temperature (OGT) of 60 degrees C, is reported. By systematically comparing this genomic sequence with the other known genomic sequences of archaea, all possessing higher OGT, a number of strong correlations have been identified between characteristics of genomic organization and the OGT. With increasing OGT, in the genomic DNA, frequency of clustering purines and pyrimidines into separate dinucleotides rises (e.g., by often forming AA and TT, whereas avoiding TA and AT). Proteins coded in a genome are divided into two distinct subpopulations possessing isoelectric points in different ranges (i.e., acidic and basic), and with increasing OGT the size of the basic subpopulation becomes larger. At the metabolic level, genes coding for enzymes mediating pathways for synthesizing some coenzymes, such as heme, start missing. These findings provide insights into the design of individual genomic components, as well as principles for coordinating changes in these designs for the adaptation to new environments.
authors: National Institute of Bioscience and Human Technology, Core Research for Evolutional Science and Technology Centre of Structural Biology, 1-1 Higashi, Tsukuba 305-0046, Japan.
authors: T
authors: T
authors: Kawashima
authors: N
authors: N
authors: Amano
authors: H
authors: H
authors: Koike
authors: S
authors: S
authors: Makino
authors: S
authors: S
authors: Higuchi
authors: Y
authors: Y
authors: Kawashima-Ohya
authors: K
authors: K
authors: Watanabe
authors: M
authors: M
authors: Yamazaki
authors: K
authors: K
authors: Kanehori
authors: T
authors: T
authors: Kawamoto
authors: T
authors: T
authors: Nunoshiba
authors: Y
authors: Y
authors: Yamamoto
authors: H
authors: H
authors: Aramaki
authors: K
authors: K
authors: Makino
authors: M
authors: M
authors: Suzuki
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology*; D001482:Base Composition; D001483:Base Sequence; D003596:Cytosine; D019641:DNA, Archaeal; D019766:Genes, Archaeal; D020745:Genome, Archaeal; D006147:Guanine; D008969:Molecular Sequence Data; D013696:Temperature; D013822:Thermoplasma / Q000235:genetics*
chemical_list: D019641:DNA, Archaeal; D006147:Guanine; D003596:Cytosine