Evaluation of the bromosulfophthalein 30-minute retention test for the diagnosis of hepatic disease in dogs.
The purpose of this study was to evaluate efficacy of bromosulfophthalein (BSP) retention testing in dogs with and without histopathologically confirmed hepatobiliary disease. Medical records of 150 dogs with hepatobiliary disease having both a BSP test and hepatic biopsy were retrieved. Histopathologic slides of liver tissue were reviewed, and dogs were classified according to 1 of 11 histopathologic categories. Twenty-five clinically normal random-source dogs were used as controls for hepatic biopsy and BSP testing. No dogs suffered adverse effects due to BSP administration. BSP retention was significantly (P < .05) higher in hospitalized (13.9%) than control (3.2%) dogs, but the test could not distinguish between hospitalized dogs with different types of hepatobiliary disease. Sensitivity, specificity, and predictive values of BSP retention as a test for hepatic disease were calculated. Using 5.0% as a cutoff for normal BSP retention resulted in a specificity of 88% and a sensitivity of 76%. Using 6.0% as a cutoff for normal BSP retention resulted in a specificity of 100% and a sensitivity of 70%. Dogs of this study having BSP retention of >6% had at least an 86% chance of having an abnormal liver. We concluded that continued use of BSP retention testing is warranted as a noninvasive diagnostic test for liver disease in dogs.
Flatland
B
B
Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg 24061-0432, USA.
Leib
M S
MS
Warnick
L D
LD
Sponenberg
D P
DP
D000818:Animals; D001706:Biopsy / Q000662:veterinary; D016022:Case-Control Studies; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D005260:Female; D006651:Histocytochemistry / Q000662:veterinary; D007202:Indicators and Reagents / Q000494:pharmacology*; D008099:Liver / Q000473:pathology; D008107:Liver Diseases / Q000175:diagnosis / Q000662:veterinary*; D008111:Liver Function Tests / Q000662:veterinary*; D008297:Male; D011237:Predictive Value of Tests; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric; D013448:Sulfobromophthalein / Q000494:pharmacology
D007202:Indicators and Reagents; D013448:Sulfobromophthalein