Immunostimulant (1 --> 3)-D-glucans from the cell wall of Cryphonectria parasitica (Murr.) Barr strain 263.
A (1 --> 3)-beta-D-glucan with approximately 30% of the residues having a beta-D-Glc-(1 --> 6) branch is the main water-soluble component of the cell wall polysaccharide of Cryphonectria parasitica (Murr.) Barr strain 263. A (1 --> 3)-glucan with both alpha and beta anomeric linkages was found in the water-insoluble polysaccharide fraction. Both fractions possess immunological activity, being able to induce the production of either tumour necrosis factor alpha (TNF-alpha) or nitrite (NO2-).
Molinaro
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Dipartimento di Chimica Organica e Biologica, Università di Napoli Federico II, Naples, Italy. molinaro@unina.it
Lanzetta
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R
Mancino
A
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Evidente
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A
Di Rosa
M
M
Ianaro
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A
D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000302:isolation & purification*; D000818:Animals; D001203:Ascomycota / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000737:chemistry*; D004790:Enzyme Induction; D005936:Glucans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II; D009573:Nitrites / Q000378:metabolism; D011134:Polysaccharides / Q000032:analysis; D012995:Solubility; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014867:Water
D000276:Adjuvants, Immunologic; D005936:Glucans; D008070:Lipopolysaccharides; D009363:Neoplasm Proteins; D009573:Nitrites; D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha; D014867:Water; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse