Determination of enantiomeric purity of hyoscyamine from scopolia extract using HPLC-CD system without chiral separation.
Enantiomeric ratio of hyoscyamine from Scopolia extract was determined by chiral HPLC-CD analysis. It was found that circular dichroism (CD) detection allowed the analysis of the sample without any special pretreatment whereas UV detection required an ammonia-ether extraction. To obtain a shorter analysis time for the determination, reversed-phase HPLC-CD analysis was applied by using a g-factor calibration curve (EE% vs. CD/UV). The analysis time was shortened from 35 to 18 min. EE% values obtained were consistent with those by chiral HPLC analysis.
Kudo
K
K
JASCO Corporation, 2967-5 Ishikawa-cho, Hachioji, Tokyo 192-8537, Japan.
Iwaya
K
K
Yomota
C
C
Morris
S
S
Saito
M
M
D001285:Atropine / Q000737:chemistry / Q000302:isolation & purification*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002942:Circular Dichroism; D010936:Plant Extracts / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D010936:Plant Extracts; D001285:Atropine