Cloning and expression of MP13 gene from rat hippocampus, a new factor related to guanosine triphosphate regulation.
C-Fos and the Fos-related antigens (FRA) are induced by various stimuli. A novel 35-37 kDa FRA was induced much longer after the treatment using kainic acid (KA) and may be very important for neuronal survival after brain damage. To identify this long-term FRA, we have constructed a cDNA library derived from hippocampus after KA treatment and screened it with an antibody highly conserved M-peptide region of FRAs. One gene, MP13, was cloned with a 1662 bp open reading frame and coded for a 554-amino acid protein. MP13 has a leucine zipper region, a glutamine repeat region, and has high similarity to the activator of the small guanosine triphosphate (GTP)ase Rab5. Gel retardation analysis revealed that MP13 functions as a GTP regulation related factor.
Feng
Z
Z
Division of Neurology, University Department of Medicine, University of Hong Kong, Queen Mary Hospital, Pokfulam Road, Hong Kong, People's Republic of China.
Hong
J
J
Qi
Q
Q
Han
Y
Y
Wilson
B
B
Iadarola
M
M
Tiao
N
N
Bing
G
G
D000595:Amino Acid Sequence / Q000502:physiology; D000818:Animals; D001483:Base Sequence / Q000502:physiology; D001930:Brain Injuries / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D002470:Cell Survival / Q000502:physiology*; D003001:Cloning, Molecular / Q000379:methods; D005786:Gene Expression Regulation / Q000502:physiology; D006160:Guanosine Triphosphate / Q000378:metabolism*; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D007608:Kainic Acid / Q000009:adverse effects; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D016760:Proto-Oncogene Proteins c-fos; C419704:Rabep2 protein, rat; D014157:Transcription Factors; D006160:Guanosine Triphosphate; D007608:Kainic Acid