Constitutive signaling by Kaposi's sarcoma-associated herpesvirus G-protein-coupled receptor desensitizes calcium mobilization by other receptors.
We coexpressed Kaposi's sarcoma-associated herpesvirus G protein-coupled receptors (KSHV-GPCRs) with thyrotropin-releasing hormone (TRH) receptors or m1-muscarinic-cholinergic receptors in Xenopus oocytes and in mammalian cells. In oocytes, KSHV-GPCR expression resulted in pronounced (81%) inhibition (heterologous desensitization) of Ca(2+)-activated chloride current responses to TRH and acetylcholine. Similar inhibitions of cytoplasmic free Ca(2+) responses to TRH were observed in human embryonic kidney HEK 293 EM cells and in mouse pituitary AtT20 cells. Further study of oocytes showed that this inhibition was partially reversed by interferon-gamma-inducible protein 10 (IP-10), an inverse agonist of KSHV-GPCR. The basal rate of (45)Ca(2+) efflux in oocytes expressing KSHV-GPCRs was 4.4 times greater than in control oocytes, and IP-10 rapidly inhibited increased (45)Ca(2+) efflux. In the absence of IP-10, growth-related oncogene alpha caused a further 2-fold increase in (45)Ca(2+) efflux. In KSHV-GPCR-expressing oocytes, responses to microinjected inositol 1,4,5-trisphosphate were inhibited by 74%, and this effect was partially reversed by interferon-gamma-inducible protein 10. Treatment with thapsigargin suggested that the pool of calcium available for mobilization by TRH was decreased in oocytes coexpressing KSHV-GPCRs. These results suggest that constitutive signaling by KSHV-GPCR causes heterologous desensitization of responses mediated by other receptors, which signal via the phosphoinositide/calcium pathway, which is caused by depletion of intracellular calcium pools.
Lupu-Meiri
M
M
Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv 69978, Israel.
Silver
R B
RB
Simons
A H
AH
Gershengorn
M C
MC
Oron
Y
Y
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D002478:Cells, Cultured; D054357:Chemokine CXCL10; D019743:Chemokines, CXC / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004594:Electrophysiology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D019288:Herpesvirus 8, Human / Q000378:metabolism*; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D051379:Mice; D009865:Oocytes / Q000378:metabolism; D043643:Receptor, Muscarinic M1; D011956:Receptors, Cell Surface / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled; D011976:Receptors, Muscarinic / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D015398:Signal Transduction; D019284:Thapsigargin / Q000494:pharmacology; D013973:Thyrotropin-Releasing Hormone / Q000378:metabolism; D013997:Time Factors; D014981:Xenopus
C086088:CHRM1 protein, human; D054357:Chemokine CXCL10; D019743:Chemokines, CXC; D002712:Chlorides; D004791:Enzyme Inhibitors; C109334:GPR1 protein, S cerevisiae; D043643:Receptor, Muscarinic M1; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D011976:Receptors, Muscarinic; D029701:Saccharomyces cerevisiae Proteins; D013973:Thyrotropin-Releasing Hormone; D019284:Thapsigargin; D007371:Interferon-gamma; D000109:Acetylcholine; D002118:Calcium