title: Commitment to the production of male and female gametocytes in the human malaria parasite Plasmodium falciparum.
abstract: Commitment to the production of female and male gametocytes was studied in the NF54 line of the human malaria parasite Plasmodium falciparum. The development of sibling parasites derived from individual schizonts was followed, and 2 antisera against the female gametocyte-specific protein Pfg377 and the male gametocyte-specific protein alpha-tubulin II were used to determine the sex of sibling gametocytes. The experiment showed that individual cohorts of sibling gametocytes were stained in a mutually exclusive fashion by only one or the other antiserum, indicating that individual schizonts committed to yield sexual parasite progeny produce gametocytes of the same sex. This work suggests that in P. falciparum commitment to sexual differentiation occurs prior to schizont maturation, at the same moment when the sex of the resulting gametocytes is determined.
authors: Laboratorio di Biologia Cellulare, Istituto Superiore di Sanità, Rome, Italy.
authors: F
authors: F
authors: Silvestrini
authors: P
authors: P
authors: Alano
authors: J L
authors: JL
authors: Williams
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000737:chemistry; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D016778:Malaria, Falciparum / Q000469:parasitology*; D008297:Male; D008954:Models, Biological; D010963:Plasmodium falciparum / Q000235:genetics* / Q000502:physiology; D015800:Protozoan Proteins / Q000032:analysis / Q000276:immunology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012732:Sex Determination Analysis / Q000379:methods*; D019849:Sex Determination Processes; D014404:Tubulin / Q000032:analysis / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D015800:Protozoan Proteins; D014404:Tubulin