Genetic analysis of vesicular stomatitis virus-New Jersey from the 1995 outbreak in the western United States.
OBJECTIVE
To compare molecular associations between the vesicular stomatitis virus (VSV)-New Jersey isolates of the 1995 outbreak with those from previous outbreaks between 1982 and 1985 in the western United States.


METHODS
23 virus isolates considered representative of the 1995 outbreak of vesicular stomatitis.


METHODS
Viral gene coding for surface-envelope protein G was evaluated by use of nucleotide sequencing and phylogenetic analysis.


RESULTS
Changes in up to 0.77% of the nucleotide bases and 1.35% of the amino acids were detected among the 1995 viral isolates, whereas changes in up to 3.2 and 2.9% of the nucleotides and amino acids, respectively, were found, compared with the 1982 to 1985 viruses. Insertions or deletions were not found in the entire gene, which spanned 1,554 nucleotide bases.


CONCLUSIONS
Phylogenetic analysis indicated that the 1995 VSV-New Jersey belongs to a lineage distinct from that of the 1982 to 1985 viruses that caused previous outbreaks in the western United States. Furthermore, it also is distinct from strains from Central America and from the Georgian Hazelhurst strain.
Llewellyn
Z N
ZN
Department of Environmental Health, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523-1676, USA.
Ou
X
X
Chang
G J
GJ
Schmitt
B
B
Salman
M D
MD
Akkina
R K
RK
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000821:virology*; D004279:DNA, Viral / Q000737:chemistry; D004196:Disease Outbreaks / Q000662:veterinary*; D006734:Horse Diseases / Q000453:epidemiology / Q000821:virology*; D006736:Horses; D008562:Membrane Glycoproteins; D008969:Molecular Sequence Data; D010802:Phylogeny; D011897:Random Allocation; D018353:Rhabdoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D013280:Stomatitis / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D014481:United States / Q000453:epidemiology; D014721:Vesicular stomatitis Indiana virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D014759:Viral Envelope Proteins / Q000235:genetics
D004279:DNA, Viral; C028391:G protein, vesicular stomatitis virus; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins