Synthesis and biophysical characterization of tRNA(Lys,3) anticodon stem-loop RNAs containing the mcm(5)s(2)U nucleoside.
[reaction: see text] Phosphoramidite reagents of the naturally occurring modified nucleosides mcm(5)s(2)U and mcm(5)U were synthesized and along with pseudouridine were incorporated into 17-nucleotide lysine tRNA anticodon stem-loop domains. Standard RNA phosphoramidite coupling chemistry allowed us to systematically investigate the thermodynamic effects of nucleoside modification and to correlate thermodynamic trends with qualitative structure effects seen by NMR spectroscopy.
Bajji
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Department of Medicinal Chemistry, University of Utah, Salt Lake City, Utah 84112, USA.
Davis
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D000926:Anticodon; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D009705:Nucleosides / Q000138:chemical synthesis / Q000737:chemistry*; D011560:Pseudouridine / Q000737:chemistry; D012357:RNA, Transfer, Lys / Q000138:chemical synthesis* / Q000737:chemistry; D013816:Thermodynamics
D000926:Anticodon; D007202:Indicators and Reagents; D009705:Nucleosides; D012357:RNA, Transfer, Lys; D011560:Pseudouridine