Changes in adipose tissue hormone-sensitive lipase activity and cAMP during ethanol withdrawal.
The time course of the effects of ethanol withdrawal on brown and white adipose tissue hormone-sensitive lipase, cAMP production, and phosphodiesterase have been investigated after chronic drinking or liquid diet schedules. Chronic drinking significantly reduced brown adipose tissue hormone-sensitive lipase activity and cAMP levels from control. During withdrawal, there was a rebound increase to 200% control, peaking 9 h into withdrawal. White adipose tissue hormone-sensitive lipase activity and cAMP accumulation were significantly raised by both treatment schedules. Ethanol liquid diet produced a significant fall in adipose tissue hormone-sensitive lipase activity and cAMP accumulation. In brown fat, there was a rebound increase in hormone-sensitive lipase activity and cAMP; in white fat, no rebound was observed. In brown fat, the reductions in hormone-sensitive lipase activity and cAMP accumulation after chronic drinking coincided with an increase in phosphodiesterase activity. In white fat, the rise in cAMP and hormone-sensitive lipase activation coincided with a decrease in phosphodiesterase activity. We conclude that the effects of chronic ethanol on hormone-sensitive lipase activity are cAMP-dependent and mediated via alterations in phosphodiesterase activity.
Shih
M F
MF
Department of Pharmacology, University of Bristol, School of Medical Sciences, University Walk, BS8 1TD, Bristol, UK.
Taberner
P V
PV
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism*; D000273:Adipose Tissue / Q000201:enzymology*; D000818:Animals; D002492:Central Nervous System Depressants / Q000009:adverse effects / Q000097:blood*; D000242:Cyclic AMP / Q000378:metabolism*; D054684:Cyclic Nucleotide Phosphodiesterases, Type 3; D000431:Ethanol / Q000009:adverse effects / Q000097:blood*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D002787:Sterol Esterase / Q000378:metabolism*; D013375:Substance Withdrawal Syndrome / Q000378:metabolism*
D002492:Central Nervous System Depressants; D000431:Ethanol; D000242:Cyclic AMP; D002787:Sterol Esterase; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D054684:Cyclic Nucleotide Phosphodiesterases, Type 3