Characterisation of an endogenous bombesin receptor in CHO/DG44 cells.
Bombesin and its receptors have been shown to have a role regulating circadian rhythms in the hamster suprachiasmatic and dorsal raphe nuclei and have been implicated in the regulation of sleep. We have identified and characterised a bombesin receptor endogenously expressed in a Chinese hamster ovary cell line (CHO/DG44). Using a range of bombesin-like peptides, we demonstrate that this receptor displays bombesin BB2 receptor-like pharmacology. We also show that this receptor signals through inositol-[1,4,5]-trisphosphate and protein kinase C and thus provides a useful model system to aid in the interpretation of hamster suprachiasmatic nucleus studies of mammalian circadian rhythm.
Brough
S J
SJ
Neuroscience Research, SmithKline Beecham Pharmaceuticals, New Frontiers Science Park, Third Avenue, Essex CM19 5AW, Harlow, UK.
Jerman
J C
JC
Jewitt
F
F
Smart
D
D
D000818:Animals; D001839:Bombesin / Q000494:pharmacology*; D016466:CHO Cells / Q000187:drug effects* / Q000378:metabolism; D006224:Cricetinae; D018004:Receptors, Bombesin / Q000187:drug effects* / Q000378:metabolism
D018004:Receptors, Bombesin; D001839:Bombesin