[Rapid detection of Enterovirus by a direct method of polymerase chain reaction].
For the detection of Enterovirus, we devised a direct economical method of polymerase chain reaction which does not require a previous extraction of ribonucleic acid and uses infected cell culture supernatants. The system was developed on the basis of universal primers of Enterovirus and specific primers of vaccinal strain Sabin 1. The achieved results proved that the non-existence of methods of RNA extraction and purification prior to the reaction does not affect the susceptibility and specificity of the system, in the rapid detection of Enterovirus genomes and identification of vaccinal strains of poliovirus.
Sarmiento Pérez
L
L
ciipk@ipk.sld.cu
Avalos Redón
I
I
Ramos Valdés
Y
Y
Más Lago
P
P
Bello Corredor
M
M
Palomera Puentes
R
R
Barrios Olivera
J
J
D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D004770:Enterovirus / Q000235:genetics / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000175:diagnosis / Q000821:virology; D006801:Humans; D008590:Meningoencephalitis / Q000175:diagnosis / Q000821:virology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000235:genetics; D012680:Sensitivity and Specificity
D004279:DNA, Viral; D012367:RNA, Viral