Nuclear factor 1 (NF1) affects accurate termination and multiple-round transcription by human RNA polymerase III.
We have shown previously that the TFIIIC1/TFIIIC1' fraction interacts specifically with the VA1 terminator regions to affect both termination and initiation/reinitiation of transcription by human RNA polymerase III. Here, we further purified the VA1 terminator-binding factor to apparent homogeneity and found, by peptide sequence analysis, that it belongs to the NF1 protein family. NF1 interacts specifically with the NF1-binding sites within the terminator regions of the VA1 gene and with two subunits (TFIIIC220 and TFIIIC110) of human TFIIIC2. Immunodepletion with anti-NF1 antibodies dramatically decreases transcription from the VA1 template in nuclear extract, and mutation at the NF1-binding site in the terminator region of the VA1 gene selectively affects multiple-round transcription (reinitiation of transcription) and termination. In addition, NF1 acts in conjunction with TFIIIC to promote accurate termination by RNA polymerase III on a C-tailed VA1 template.
Wang
Z
Z
The Laboratory of Biochemistry and Molecular Biology,The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.
Bai
L
L
Hsieh
Y J
YJ
Roeder
R G
RG
D001483:Base Sequence; D001665:Binding Sites; D002467:Cell Nucleus / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D051839:NFI Transcription Factors; D009687:Nuclear Proteins / Q000378:metabolism; D015345:Oligonucleotide Probes; D012320:RNA Polymerase III / Q000378:metabolism*; D013698:Templates, Genetic; D013728:Terminator Regions, Genetic; D014157:Transcription Factors / Q000378:metabolism*; D019474:Transcription Factors, TFIII / Q000378:metabolism; D014158:Transcription, Genetic; D000098832:Transcription Factor TFIIIC
D004268:DNA-Binding Proteins; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D015345:Oligonucleotide Probes; D014157:Transcription Factors; D019474:Transcription Factors, TFIII; D000098832:Transcription Factor TFIIIC; C080333:transcription factor nuclear factor 1; D012320:RNA Polymerase III