title: NASA/American Cancer Society High-Resolution Flow Cytometry Project-I.
abstract: BACKGROUND
  The NASA/American Cancer Society (ACS) flow cytometer can simultaneously analyze the electronic nuclear volume (ENV) and DNA content of cells. This study describes the schematics, resolution, reproducibility, and sensitivity of biological standards analyzed on this unit.
  
  
  METHODS
  Calibrated beads and biological standards (lymphocytes, trout erythrocytes [TRBC], calf thymocytes, and tumor cells) were analyzed for ENV versus DNA content. Parallel data (forward scatter versus DNA) from a conventional flow cytometer were obtained.
  
  
  RESULTS
  ENV linearity studies yielded an R value of 0.999. TRBC had a coefficient of variation (CV) of 1.18 +/- 0.13. DNA indexes as low as 1.02 were detectable. DNA content of lymphocytes from 42 females was 1.9% greater than that for 60 males, with a noninstrumental variability in total DNA content of 0.5%. The ENV/DNA ratio was constant in 15 normal human tissue samples, but differed in the four animal species tested. The ENV/DNA ratio for a hypodiploid breast carcinoma was 2.3 times greater than that for normal breast tissue.
  
  
  CONCLUSIONS
  The high-resolution ENV versus DNA analyses are highly reliable, sensitive, and can be used for the detection of near-diploid tumor cells that are difficult to identify with conventional cytometers. ENV/DNA ratio may be a useful parameter for detection of aneuploid populations.
authors: RATCOM, Inc., Miami, Florida 33193, USA. ratcom@bellsouth.net
authors: R A
authors: RA
authors: Thomas
authors: A
authors: A
authors: Krishan
authors: D M
authors: DM
authors: Robinson
authors: C
authors: C
authors: Sams
authors: F
authors: F
authors: Costa
mesh_terms: D000570:American Cancer Society; D000818:Animals; D002138:Calibration; D002467:Cell Nucleus / Q000473:pathology*; D004247:DNA / Q000032:analysis*; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D006801:Humans; D007962:Leukocytes / Q000166:cytology / Q000378:metabolism; D008297:Male; D009369:Neoplasms / Q000235:genetics / Q000473:pathology*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013950:Thymus Gland / Q000166:cytology / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014481:United States; D018557:United States National Aeronautics and Space Administration
chemical_list: D004247:DNA