Macrophages express neurotrophins and neurotrophin receptors. Regulation of nitric oxide production by NT-3.
In this study we examined the expression of neurotrophins and their receptors in mouse macrophages and the effects of the neurotrophins on nitric oxide secretion. Macrophages expressed TrkB and TrkC but not BDNF, NT-3 or NT-4. LPS induced up-regulation of TrkB and TrkC and of BDNF and NT-3 expression. Treatment of macrophages with NT-3 increased the secretion of nitric oxide in LPS-treated macrophages and this increase was blocked by K252a, a Trk kinase inhibitor. In contrast, BDNF and NT-4 had no significant effects on the induction of nitric oxide. Our results suggest that NT-3 play important roles in the function of macrophages during inflammatory responses and in tissue repair.
Barouch
R
R
Gonda (Goldschmied) Medical Diagnosis Research Center, Faculty of Life Sciences, Bar-Ilan University, 52900, Ramat Gan, Israel.
Appel
E
E
Kazimirsky
G
G
Brodie
C
C
D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000502:physiology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D020933:Neurotrophin 3 / Q000502:physiology*; D009569:Nitric Oxide / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000032:analysis; D020813:Receptor, trkB / Q000235:genetics / Q000502:physiology*; D020812:Receptor, trkC / Q000235:genetics / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D019208:Brain-Derived Neurotrophic Factor; D008070:Lipopolysaccharides; D020933:Neurotrophin 3; D012333:RNA, Messenger; D009569:Nitric Oxide; D020813:Receptor, trkB; D020812:Receptor, trkC