title: Cytokine production from peripheral whole blood in atopic and nonatopic asthmatics: relationship with blood and sputum eosinophilia and serum IgE levels.
abstract: BACKGROUND
  The cytokine network is thought to be essential in orchestrating airway inflammation in asthma. Although evidence has accumulated to suggest that atopic asthma is a Th2 disease, much less is known about nonatopic asthma.
  
  
  METHODS
  We have compared the production of IL-4, IL-6, IFN-gamma, and TNF-alpha from peripheral blood leukocytes between atopic (n=21) and nonatopic (n=22) asthmatics and healthy nonatopic subjects (n=20). Peripheral blood was incubated for 24 h either without stimulus or with LPS or PHA. Cytokines were measured by the immunotrapping technique (Dynamic Immunoassay).
  
  
  RESULTS
  When compared to healthy nonatopic subjects, both atopic and nonatopic asthmatics showed increased blood and sputum eosinophilia associated with raised total serum IgE levels. Similarly, both asthma groups displayed spontaneous, endotoxin-induced overproduction of IL-6. Enhanced spontaneous, endotoxin-induced release of IL-4 combined with reduced spontaneous IFN-gamma production was seen only in atopic asthma. In this group of patients, the production of IL-4 was related to the extent of blood and sputum eosinophilia. In nonatopic asthmatics, serum levels of IgE were inversely related to the production of IFN-gamma.
  
  
  CONCLUSIONS
  Both atopic and intrinsic asthma display raised blood and airway eosinophilia, raised total serum IgE, and overproduction of IL-6 from peripheral blood. Atopic asthma is also characterized by impaired spontaneous release of IFN-gamma and increased production of IL-4 that correlates with the magnitude of eosinophilic inflammation.
authors: Department of Pneumology, CHU Sart-Tilman, University of Liège, Belgium.
authors: J
authors: J
authors: Bettiol
authors: P
authors: P
authors: Bartsch
authors: R
authors: R
authors: Louis
authors: D
authors: D
authors: De Groote
authors: Y
authors: Y
authors: Gevaerts
authors: E
authors: E
authors: Louis
authors: M
authors: M
authors: Malaise
mesh_terms: D000328:Adult; D001249:Asthma / Q000097:blood / Q000276:immunology* / Q000503:physiopathology; D016207:Cytokines / Q000097:blood*; D005260:Female; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000097:blood / Q000276:immunology* / Q000503:physiopathology; D007073:Immunoglobulin E / Q000097:blood*; D007958:Leukocyte Count; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D011657:Pulmonary Eosinophilia / Q000097:blood / Q000276:immunology* / Q000503:physiopathology; D012129:Respiratory Function Tests; D013183:Sputum / Q000166:cytology / Q000276:immunology
chemical_list: D016207:Cytokines; D007073:Immunoglobulin E