Analysis of expression of flagella by Salmonella enterica serotype typhimurium by monoclonal antibodies recognising both phase specific and common epitopes.
Monoclonal antibodies specific for phase 1 ("i" antigen), phase 2 ("1,2" antigen) and common epitopes of the flagellins of Salmonella enterica serotype Typhimurium were raised. Having confirmed their specificity, the monoclonal antibodies were used to develop semi-quantitative ELISAs in order to assess the relative expression of the two phases by strains of Typhimurium. The majority of Typhimurium strains representative of a wide cross-section of definitive types from animal and environmental sources preferentially expressed phase 1 antigen in vitro. DT40 strains were unique in expressing phase 2 preferentially. The ratio of phase 1 to phase 2 expressed by strains tended to be constant for any one strain when strains were grown on a number of conventional laboratory media. However, the ratio of phases was shown to be modulated by incubation at 42 degrees C and buffering media at pH values, notably 4.5, other than neutral. Selenite broth and Rambach media repressed flagellation.
Sojka
M
M
Department of Bacterial Diseases, Veterinary Laboratory Agency (Weybridge), Addlestone, Surrey KT15 3NB, UK.
Sayers
A R
AR
Woodward
M J
MJ
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000942:Antigens, Bacterial / Q000096:biosynthesis / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D005407:Flagella / Q000276:immunology / Q000378:metabolism*; D005408:Flagellin / Q000096:biosynthesis* / Q000276:immunology; D006863:Hydrogen-Ion Concentration; D012486:Salmonella typhimurium / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000939:Epitopes; D005408:Flagellin