title: [Horseradish peroxidase retrograde labeling of primary sensory axons in rats: a comparison between three different intraspinal injections methods].
abstract: OBJECTIVE
  In order to improve the results of intraspinal retrograde labeling of post-ganglionic primary sensory axons by horseradish peroxidase (HRP), the authors compared three different intraspinal injection methods of this tracer into the inferior thoracic spinal cord in the rat.
  
  
  METHODS
  'Open field' method (group 1, N = 8); stereotactic injection, needle tip diameter = 0.72 mm (group 2, N = 8); stereotactic injection, needle tip diameter = 0.24 mm (group 3, N = 8). Histological features of the spinal injection site showed that tissue damages due to injection was more extensive and deeper than expected. HRP transported in retrograde fashion from injection site to sensory body cells located in dorsal root ganglia (DRG) was revealed by the Mesulam histochemical technique.
  
  
  RESULTS
  The mean number of labeled neurons per DRG was 652 in group 3, 116 in group 2, and 77 in group 1. Differences were statistically significant, especially between groups 1 and 3 (P = 4.10(-16)) and groups 2 and 3 (P = 2.10(-17)).
  
  
  CONCLUSIONS
  Retrograde labeling of primary sensory axons by HRP (or another axonal tracer) with fine needle stereotactic intraspinal injection may represent an alternative to anterograde labeling. This reliable and reproducible method may be useful in studies dealing with regeneration of post-ganglionic primary sensory axons.
authors: Laboratoire de neurochirurgie expérimentale, centre hospitalier universitaire de Bicêtre, Le Kremlin-Bicêtre, France.
authors: P
authors: P
authors: Dam Hieu
authors: S
authors: S
authors: Liu
authors: A S
authors: AS
authors: Bonnard
authors: N
authors: N
authors: Boisset
authors: E
authors: E
authors: Genty
authors: M
authors: M
authors: Tadié
mesh_terms: D000818:Animals; D001338:Autonomic Fibers, Postganglionic / Q000648:ultrastructure*; D001369:Axons / Q000648:ultrastructure*; D006651:Histocytochemistry / Q000379:methods*; D006735:Horseradish Peroxidase / Q000008:administration & dosage*; D007278:Injections, Spinal / Q000295:instrumentation / Q000379:methods*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling / Q000379:methods*; D013238:Stereotaxic Techniques / Q000295:instrumentation
chemical_list: D006735:Horseradish Peroxidase