Deletion mapping of 14q32 in human neuroblastoma defines an 1,100-kb region of common allelic loss.
BACKGROUND
We analyzed loss of heterozygosity (LOH) in 54 primary neuroblastomas (NBs) using 12 microsatellite markers on 14q32, and found that 31% (17/54) NBs showed LOH.


METHODS
The smallest region of overlap (SRO) was identified between D14S62 and D14S987.


RESULTS
There was no statistical correlation between LOH and any clinicopathologic features, including age, stage, amplification of MYCN, and ploidy. A sequence-ready bacterial artificial chromosome (BAC) contig was constructed, and the minimum tiling path of six BACs covered the SRO; the physical length of this region was no larger than 1,000 kb.


CONCLUSIONS
Our findings support the existence of a putative tumor-related gene on 14q32 for the tumorigenesis of NB.
Hoshi
M
M
Department of Molecular Pathology, Tohoku University School of Medicine, Sendai, Japan.
Shiwaku
H O
HO
Hayashi
Y
Y
Kaneko
Y
Y
Horii
A
A
D000483:Alleles; D002648:Child; D002675:Child, Preschool; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics*; D016147:Genes, Tumor Suppressor / Q000235:genetics*; D006801:Humans; D007223:Infant; D019656:Loss of Heterozygosity; D009447:Neuroblastoma / Q000235:genetics*