Multicolor fluorescence in situ hybridization with peptide nucleic acid probes for enumeration of specific chromosomes in human cells.
In previous studies, we showed that peptide nucleic acid (PNA) probes have significant advantages over conventional synthetic RNA or DNA probes in FISH procedures for detecting telomeric and trinucleotide repeat sequences. Here, we report that directly labeled PNA probes recognizing chromosome-specific repeat sequences are also powerful tools for detecting and enumerating specific chromosomes in interphase and metaphase cells. This is illustrated by multicolor FISH experiments with cells from normal individuals and patients with numerical sex chromosome aberrations.
Taneja
K L
KL
Boston Probes, Bedford, Massachusetts 01730, USA. ktaneja@bostonprobes.com
Chavez
E A
EA
Coull
J
J
Lansdorp
P M
PM
D002232:Carbocyanines; D002851:Chromatography, High Pressure Liquid; D002877:Chromosomes, Human / Q000737:chemistry* / Q000235:genetics; D005260:Female; D005347:Fibroblasts / Q000737:chemistry; D005456:Fluorescent Dyes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008214:Lymphocytes / Q000737:chemistry; D008297:Male; D015341:Nucleic Acid Probes; D020135:Peptide Nucleic Acids / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
D002232:Carbocyanines; D005456:Fluorescent Dyes; D015341:Nucleic Acid Probes; D020135:Peptide Nucleic Acids; C089187:cyanine dye 3; C085321:cyanine dye 5