Inhibitory effects of an antisense oligonucleotide in an experimentally infected mouse model of influenza A virus.
The antiviral effects of a 20-mer antisense phosphorothioate oligonucleotide, PB2-as, on influenza A virus infection in mice were examined and compared to those of PB2-as encapsulated with several cationic liposomes. Intravenous injection of PB2-as, as a complex with DMRIE-C, a cationic liposome, was most effective for prolonging the mean survival time in days (MSDs) and increasing the survival rates of mice infected with the influenza A virus. In addition, the liposomal PB2-as significantly inhibited viral growth in lung tissues. These results suggest that PB2-as encapsulated with DMRIE-C may be active against the influenza A virus infection through the inhibition of virus replication in the mouse lung.
Mizuta
T
T
Department of Microbiology, Fukushima University School of Medicine, 1 Hikarigaoka, Fukushima 960-1295, Japan.
Fujiwara
M
M
Abe
T
T
Miyano-Kurosaki
N
N
Yokota
T
T
Shigeta
S
S
Takaku
H
H
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001483:Base Sequence; D017931:DNA Primers; D005260:Female; D009980:Influenza A virus / Q000302:isolation & purification*; D051379:Mice; D008807:Mice, Inbred BALB C; D016376:Oligonucleotides, Antisense / Q000494:pharmacology* / Q000627:therapeutic use; D009976:Orthomyxoviridae Infections / Q000188:drug therapy*
D000998:Antiviral Agents; D017931:DNA Primers; D016376:Oligonucleotides, Antisense