title: Effects of trilinolein on superoxide dismutase activity and mrna levels in aortic smooth muscle cells.
abstract: 1. Atherosclerotic cardiovascular disease is still the leading cause of death in Western countries. Oxygen free radicals are considered to be intimately involved in the development of atherosclerosis. Anti-oxidants may help to protect mammalian cells from the damage induced by these reactive oxygen species. Many reports have indicated that anti-oxidants used in the treatment or prevention of disease could modify the levels of superoxide dismutase (SOD). However, the effects of long-term anti-oxidant treatment on the levels of SOD in smooth muscle cells (SMC) is still unclear. In the present study, the effects of the lipophilic anti-oxidant trilinolein on the activity and gene expression of SOD in SMC were evaluated. 2. After 2 days incubation with 0.1 micromol/L trilinolein, the activity and mRNA levels of SOD were increased in rat aortic SMC (A7r5), but there was no significant change in these parameters with a higher concentration of 1 micromol/L trilinolein. 3. In contrast, after 7 days incubation with trilinolein, both the activity and mRNA levels of SOD were lowered in a dose-dependent manner. 4. These data emphasize the importance of choosing an optimal dosage for supplementation with anti-oxidants in humans for the scavenging of oxygen free radicals.
authors: Division of Cardiovascular Medicine and Surgery, Wan Fang Hospital, Taiwan.
authors: P
authors: P
authors: Chan
authors: J J
authors: JJ
authors: Chang
authors: F C
authors: FC
authors: Chen
authors: M S
authors: MS
authors: Wu
authors: B
authors: B
authors: Tomlinson
authors: J T
authors: JT
authors: Cheng
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001013:Aorta, Thoracic / Q000166:cytology; D015153:Blotting, Western; D002460:Cell Line; D009130:Muscle, Smooth / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D013482:Superoxide Dismutase / Q000378:metabolism*; D014280:Triglycerides / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D012333:RNA, Messenger; D014280:Triglycerides; D013482:Superoxide Dismutase; C009564:trilinolein