Hepatitis B virus Dane particles bind to human plasma apolipoprotein H.
Human apolipoprotein H (apo H) was found to bind specifically to hepatitis B surface antigen (HBsAg) from hepatitis B virus (HBV)-infected individuals. We used recombinant HBsAg proteins to analyze HBV domains recognized by apo H. We showed that the myristylated pre-S1 domain of HBsAg strongly interacted with apo H. This binding involved phospholipid components of the HBV envelope because their removal by detergent prevented apo H-HBsAg interaction. The opposite effects of iron and zinc metal ions on binding suggest that the oxidation of phospholipids also affects apo H-HBsAg interaction. After fractionation of viral particles on a sucrose gradient, and their addition to microtiter plates coated with apo H or anti-HBsAg, we observed that the maximal anti-HBsAg capture activity corresponded to a sucrose concentration of 36%, whereas the maximal apo H capture activity corresponded to a concentration of 39%. Electron microscopy and polymerase chain reaction (PCR) Southern blot studies of these fractions showed that the fraction with maximal apo H binding predominantly contained full Dane particles. Finally, we studied apo H-HBsAg binding relative to the presence of hepatitis B virus markers and observed that apo H binding activity for HBsAg was higher in sera from patients in the active virus replication phase.
Stefas
I
I
Laboratoire d'Immunologie Rétrovirale et Moléculaire IRD UR34, Centre Régional de Transfusion Sanguine, Montpellier, France. stefas@melusine.mpl.orstrom.fr
Rucheton
M
M
D'Angeac
A D
AD
Morel-Baccard
C
C
Seigneurin
J M
JM
Zarski
J P
JP
Martin
M
M
Cerutti
M
M
Bossy
J P
JP
Missé
D
D
Graafland
H
H
Veas
F
F
D000818:Animals; D015139:Blotting, Southern; D002460:Cell Line; D004279:DNA, Viral / Q000378:metabolism; D006023:Glycoproteins / Q000097:blood / Q000378:metabolism*; D006514:Hepatitis B Surface Antigens / Q000097:blood / Q000737:chemistry / Q000378:metabolism; D006515:Hepatitis B virus / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D010084:Oxidation-Reduction; D010743:Phospholipids / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000097:blood / Q000737:chemistry / Q000378:metabolism; D018411:Spodoptera / Q000166:cytology; D053482:beta 2-Glycoprotein I
D004279:DNA, Viral; D006023:Glycoproteins; D006514:Hepatitis B Surface Antigens; D046911:Macromolecular Substances; D010743:Phospholipids; D011994:Recombinant Proteins; D053482:beta 2-Glycoprotein I