title: Characterization of two distinct DP-related genes from Arabidopsis thaliana.
abstract: E2F/DP complexes play a pivotal role in the regulation of the G1/S transition in animals. Recently, plant E2F homologs have been cloned, but DP-related sequences have not been identified so far. Here we report that Arabidopsis thaliana contains at least two different DP-related genes, AtDPa and AtDPb. They exhibit an overall domain organization similar to that of their animal counterparts, although phylogenetic analysis demonstrated that they form a separate subgroup. AtDPs efficiently heterodimerize in vitro with the Arabidopsis E2F-related proteins, AtE2Fa and AtE2Fb through their dimerization domains. AtDPa and AtE2Fa are predominantly produced in actively dividing cells with highest transcript levels in early S phase cells.
authors: Vakgroep Moleculaire Genetica, Departement Plantgenetica, Vlaams Interuniversitair voor Biotechnologie (VIB), Universiteit Gent, Belgium.
authors: Z
authors: Z
authors: Magyar
authors: A
authors: A
authors: Atanassova
authors: L
authors: L
authors: De Veylder
authors: S
authors: S
authors: Rombauts
authors: D
authors: D
authors: Inzé
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000235:genetics*; D001665:Binding Sites; D002352:Carrier Proteins; D002453:Cell Cycle; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D019281:Dimerization; D050684:E2F Transcription Factors; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D009928:Organ Specificity; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011233:Precipitin Tests; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D056465:Retinoblastoma-Binding Protein 1; D016415:Sequence Alignment; D050680:Transcription Factor DP1; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; C417206:DP transcription factor, plant; D050684:E2F Transcription Factors; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D056465:Retinoblastoma-Binding Protein 1; D050680:Transcription Factor DP1; D014157:Transcription Factors