PEA3 and AP-1 are required for constitutive IL-8 gene expression in hepatoma cells.
Interleukin-8 (IL-8) mRNA was constitutively expressed in human hepatoma cell line, HepG2 and in human hepatocellular carcinoma (HCC), which often form hypervascular tumors. The sequence 5'-AGGAAG-3' at -137 to -132 bp of IL-8 promoter was shown to be polyomavirus enhancer A binding protein-3 (PEA3) binding site, which can cooperate with activator protein-1 (AP-1). Both PEA3 and AP-1 are essential for constitutive IL-8 expression in HepG2 cells, determined by promoter assays. Moreover, PEA3 and IL-8 proteins coexisted in HCC tissues, but not in uninvolved liver tissues. It is possible PEA3 may have important roles in tumor progression and in angiogenesis in HCC.
Iguchi
A
A
Department of Laboratory and Molecular Medicine, Kagoshima University School of Medicine, Japan.
Kitajima
I
I
Yamakuchi
M
M
Ueno
S
S
Aikou
T
T
Kubo
T
T
Matsushima
K
K
Mukaida
N
N
Maruyama
I
I
D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D002460:Cell Line; D017931:DNA Primers; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D016209:Interleukin-8 / Q000235:genetics*; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D018808:Transcription Factor AP-1 / Q000502:physiology*; D014157:Transcription Factors / Q000502:physiology*
D017931:DNA Primers; D016209:Interleukin-8; D018808:Transcription Factor AP-1; D014157:Transcription Factors; C065788:transcription factor PEA3