[Monoclonal antibodies to dengue virus 4: the spectrum of the reactivity to 4 serotypes of dengue].
These types of monoclonal antibodies 8E8, 3F7 and 1E9 to dengue 4 virus H-241 strain. These monoclonal antibodies show various patterns of reactivity to the four dengue serotypes and different antigen preparations of serotype 4 when they were tested in various serological methods. The monoclonal antibody 8E8 exhibited a specificity of serotype (D-2; by hemagglutination inhibition); subcomplex (D-2 and D-4 by immunofluorescence) and complex (by immunoperoxidase technique). It was able to neutralize by 80% homologous virus and it turned out to be the only reactive monoclonal antibody in the complement fixation test. The monoclonal 3F7 did not react to by hemagglutination inhibition, recognized serotypes D-1, D-2, D-3 and D-4 by immunofluorescence and only serotypes D1 and D4 by immunoperoxidase technique but it was unable to neutralize the homologous virus. The 1E9 antibody was reactive to serotypes D1, D-2, D-3 and D-4 only by hemagglutination inhibition and neutralized serotype D-4. All the monoclonal antibodies were able to react to various dengue antigens through an ELISA of double antibody and showed fluorescent activity against 38th pass in Beagle dog kidney culture; however, they could not react to a D-4 recombinant antigen.
Pupo Antúnez
M
M
Instituto de Medicina Tropical Pedro Kourí, Marianao, Ciudad de La Habana, Cuba. mpupo@ipk.sld.cu
Alvarez Vera
M
M
Soto Hernández
M
M
Rodríguez Sánchez
H
H
Rodríguez Díaz
R
R
Morier Díaz
L
L
Guzmán Tirado
M G
MG
D000330:Aedes / Q000821:virology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification; D000914:Antibodies, Viral / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D002478:Cells, Cultured; D003716:Dengue Virus / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006825:Hybridomas / Q000276:immunology; D007668:Kidney / Q000166:cytology; D051379:Mice; D012703:Serotyping
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral