[Evaluation of influenza virus type A detection kit].
We evaluated a new Influenza Virus Type A Detection Kit (InfluA-AD "Seiken") and compared it to virus isolation and RT-PCR using throat swab specimens. Our results were as follows: 1) From 13 specimens in which Influenza virus type A was isolated, InfluA-AD "Seiken" detected 12 while RT-PCR detected 9. 2) From 7 specimens in which Influenza virus type B was isolated, InfluA-AD "Seiken" detected 0 while RT-PCR detected 1. 3) From 2 specimens in which Adeno virus was isolated, InfluA-AD "Seiken" detected 0 and RT-PCR detected 0. 4) From 28 specimens in which no virus was isolated, InfluA-AD "Seiken" detected 6 while RT-PCR detected 8. InfluA-AD "Seiken" showed a sensitivity of 92.3% and specificity of 83.8% when compared to virus isolation. We conclude that the new test will be useful for the diagnosis of Influenza virus type A infection particularly in big commercial laboratories.
Takeuchi
Y
Y
Department of Pediatrics, Kawasaki Municipal Hospital.
Shimizu
H
H
D005069:Evaluation Studies as Topic; D007124:Immunoenzyme Techniques; D009980:Influenza A virus / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic / Q000592:standards*
D011933:Reagent Kits, Diagnostic