title: Microtubule- and dynein-mediated movement of Orientia tsutsugamushi to the microtubule organizing center.
abstract: The host cell microfilaments and microtubules (MTs) are known to play a critical role in the life cycles of several pathogenic intracellular microbes by providing for successful invasion and promoting movement of the pathogen once inside the host cell cytoplasm. Orientia tsutsugamushi, an obligate intracellular bacterium, enters host cells by induced phagocytosis, escapes to the cytosol, and then replicates in the cytosol. ECV304 cells infected with O. tsutsugamushi revealed the colocalization of the MT organizing center (MTOC) and cytosolic orientiae by indirect immunofluorescence assay. Using immunofluorescence microscopy in the presence and absence of MT-depolymerizing agents (colchicine and nocodazole), it was shown that the cytosolic oriential movement was mediated by MTs. By transfection study (overexpression of dynamitin [also called p50], which is known to associate with dynein-dependent movement), the movement of O. tsutsugamushi to the MTOC was also mediated by dynein, the minus-end-directed MT-related motor. Although the significance of this movement in the life cycle of O. tsutsugamushi was not proven, we propose that the cytosolic O. tsutsugamushi bacteria use MTs and dyneins to propel themselves from the cell periphery to the MTOC.
authors: Department of Microbiology and Immunology, Seoul National University College of Medicine, and Institute of Endemic Disease, Seoul National University Medical Research Center, 28 Yongon-dong, Chongno-gu, Seoul 110-799, Republic of Korea.
authors: S W
authors: SW
authors: Kim
authors: K S
authors: KS
authors: Ihn
authors: S H
authors: SH
authors: Han
authors: S Y
authors: SY
authors: Seong
authors: I S
authors: IS
authors: Kim
authors: M S
authors: MS
authors: Choi
mesh_terms: D002460:Cell Line; D000072159:Dynactin Complex; D004398:Dyneins / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000502:physiology; D008870:Microtubules / Q000502:physiology*; D009068:Movement; D012285:Orientia tsutsugamushi / Q000502:physiology*
chemical_list: C000606361:DCTN2 protein, human; D000072159:Dynactin Complex; D008869:Microtubule-Associated Proteins; D004398:Dyneins