Synthesis of alpha-Gal epitope derivatives with a galactosyltransferase-epimerase fusion enzyme.
Alpha-Gal epitopes are carbohydrate structures bearing an alpha-D-Galp-(1-->3)-beta-D-Galp terminus and are the main cause of antibody-mediated hyperacute rejection in xenotransplantation. Nine monosaccharides and ten disaccharides were evaluated as substrates for a fusion protein, which contains both alpha-(1-->3)-galactosyltransferase and uridine-5'-diphosphogalactose 4-epimerase. Four disaccharide and six trisaccharide alpha-Gal epitope derivatives were synthesized utilizing this novel fusion enzyme.
Fang
J
J
Department of Chemistry, Wayne State University, Detroit, MI 48202-3489, USA.
Chen
X
X
Zhang
W
W
Janczuk
A
A
Wang
P G
PG
D000818:Animals; D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005700:Galactosyltransferases / Q000235:genetics / Q000378:metabolism; D006084:Graft Rejection / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009844:Oligosaccharides / Q000138:chemical synthesis / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000138:chemical synthesis / Q000378:metabolism; D014183:Transplantation, Heterologous; D014312:Trisaccharides / Q000138:chemical synthesis / Q000276:immunology*; D014534:UDPglucose 4-Epimerase / Q000235:genetics / Q000378:metabolism
D009844:Oligosaccharides; D011993:Recombinant Fusion Proteins; D014312:Trisaccharides; C076569:alpha-galactosyl epitope; D005700:Galactosyltransferases; D014534:UDPglucose 4-Epimerase