Taxol from Tubercularia sp. strain TF5, an endophytic fungus of Taxus mairei.
The diterpenoid taxol is an important anticancer agent used widely in the clinic. The purpose of this work was to identify a taxol-producing endophytic fungus (strain TF5) isolated from Taxus mairei and study its anticancer activities. Strain TF5 was identified as a Tubercularia sp. according to the morphology of the fungal culture, the mechanism of spore production and the characteristics of the spores. Strain TF5 produced taxol, when grown in potato dextrose liquid medium and analyzed by thin layer chromatography, high performance liquid chromatography, ultraviolet and mass spectrometry. The fungal taxol, which was isolated from the organic extract of the TF5 culture, had strong cytotoxic activity towards KB and P388 cancer cells in vitro, tested by the MTT assay. Observed with immunofluorescence and electron microscopy, the fungal taxol enhanced microtubule stability and bundling in culture cells and induced tubulin polymerization in vitro similar to the authentic taxol.
Wang
J
J
The Key Laboratory of Ministry of Education for Cell Biology and Tumor Cell, Engineering and School of Life Sciences, Xiamen University, 361005, Xiamen, PR China.
Li
G
G
Lu
H
H
Zheng
Z
Z
Huang
Y
Y
Su
W
W
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D002855:Chromatography, Thin Layer; D003470:Culture Media; D006801:Humans; D006999:Hypocreales / Q000254:growth & development / Q000378:metabolism*; D007624:KB Cells; D051379:Mice; D008854:Microscopy, Electron; D008870:Microtubules / Q000187:drug effects; D017239:Paclitaxel / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D010946:Plants, Medicinal; D013172:Spores, Fungal / Q000502:physiology; D020946:Taxus / Q000382:microbiology*; D014404:Tubulin / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D003470:Culture Media; D014404:Tubulin; D017239:Paclitaxel