Posttransfusion survival (24-hour) and hemolysis of previously frozen, deglycerolized RBCs after storage at 4 degrees C for up to 14 days in sodium chloride alone or sodium chloride supplemented with additive solutions.
BACKGROUND
Previously frozen human RBCs currently are glycerolized and deglycerolized by the use of open systems that limit storage of the deglycerolized RBCs at 4 degrees C to only 24 hours.


METHODS
Healthy male volunteers who met AABB requirements for blood donors (n = 38) were studied. A volume of 450 mL of blood was collected into CPDA-1. The RBC concentrates were stored at 4 degrees C for 3 to 6 days before being frozen with 40-percent (wt/vol) glycerol and stored at -80 degrees C. The RBCs were deglycerolized, resuspended in 0.9-percent sodium chloride and 0.2-percent glucose (SG) solution or SG solution supplemented with AS-1, AS-3, or AS-5, and stored in the resuspension medium at 4 degrees C for 14 days.


RESULTS
The mean +/- SD freeze-thaw-wash process recovery was 90.0 +/- 4.0 percent for all 38 units. The mean 24-hour posttransfusion survival value was 79 percent for deglycerolized RBC stored at 4 degrees C for 7 days in SG alone, SG plus AS-3, or SG plus AS-5. Deglycerolized RBC that were stored at 4 C for 14 days in SG supplemented with AS-1, AS-3, or AS-5 had a mean 24-hour posttransfusion survival of 74 percent. After 7 days of storage of deglycerolized RBCs in SG alone, the mean hemolysis was 3. 7 percent. After 14 days of storage of deglycerolized RBCs in SG supplemented with AS-1, AS-3, or AS-5, the mean hemolysis was 2.5 percent.


CONCLUSIONS
The levels of hemolysis did not correlate with the 24-hour posttransfusion survival values.
Valeri
C R
CR
Naval Blood Research Laboratory, Boston University School of Medicine, Boston, Massachusetts 02118, USA. navblood@bu.edu
Pivacek
L E
LE
Cassidy
G P
GP
Ragno
G
G
D000225:Adenine / Q000494:pharmacology*; D001793:Blood Preservation; D001803:Blood Transfusion; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000378:metabolism*; D004905:Erythrocyte Aging / Q000502:physiology*; D004912:Erythrocytes / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology*; D005990:Glycerol / Q000378:metabolism*; D006461:Hemolysis; D006801:Humans; D008297:Male; D008353:Mannitol / Q000494:pharmacology*; D012965:Sodium Chloride / Q000494:pharmacology*; D013997:Time Factors
C037839:ADSOL; D003451:Cryoprotective Agents; D008353:Mannitol; D012965:Sodium Chloride; D005947:Glucose; D000225:Adenine; D005990:Glycerol