title: Formation of new tissue from an arteriovenous loop in the absence of added extracellular matrix.
abstract: A major requirement for the microsurgical repair of contour defects of the skin, for example, following removal of a skin cancer on the face, is a mass of vascularised subcutaneous tissue. Such tissue can be generated in vivo using basic tissue engineering principles. In previous studies in our laboratory, we have used a model comprising an arteriovenous (AV) shunt loop sandwiched in artificial dermis, placed in a cylindrical plastic growth chamber, and inserted subcutaneously to grow new connective tissue progressively up to 4 weeks. To learn more about the basic growth characteristics with this model, the same AV shunt loop within a chamber without added extracellular matrix was inserted subcutaneously into the groins of rats for 2, 4, or 12 weeks (n = 5 per group). There was a progressive increase in the mass and volume of tissue such that the chamber was two-thirds full after 12 weeks. Histological examination showed that at 2 weeks there was evidence of fibroblast and vascular outgrowth from the AV shunt, with the formation of granulation tissue, surrounded by a mass of coagulated exudate. At 4 weeks the connective tissue deposition was more extensive, with a mass of more mature granulation tissue containing considerable collagen. By 12 weeks there was an extensive, well vascularized mass of mature fibrous tissue. The blood vessels and residual adventitia of the AV shunt were the likely source of growth factors and of the cells which populated the chamber with new maturing connective tissue. A patent AV shunt in an isolated chamber appears to be the minimal requirement for the generation of new vascularized tissue that is potentially suitable for microsurgical transplantation.
authors: Bernard O'Brien Institute of Microsurgery, St Vincent's Hospital Melbourne, Fitzroy, Victoria 3065, Australia.
authors: R
authors: R
authors: Mian
authors: W A
authors: WA
authors: Morrison
authors: J V
authors: JV
authors: Hurley
authors: A J
authors: AJ
authors: Penington
authors: R
authors: R
authors: Romeo
authors: Y
authors: Y
authors: Tanaka
authors: K R
authors: KR
authors: Knight
mesh_terms: D000818:Animals; D001164:Arteriovenous Fistula; D001166:Arteriovenous Shunt, Surgical / Q000379:methods; D003238:Connective Tissue / Q000473:pathology / Q000502:physiology*; D005109:Extracellular Matrix / Q000502:physiology*; D005263:Femoral Artery; D005268:Femoral Vein; D008297:Male; D018919:Neovascularization, Physiologic; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration / Q000502:physiology