Purification and identification of a growth-stimulating peptide for Bifidobacterium bifidum from natural rubber serum powder.
Natural rubber serum powder, which is a by-product obtained in the production of latex rubber, has a strong growth-stimulating activity for Bifidobacterium bifidum JCM 1254. The retained fraction obtained by ultrafiltration (molecular weight cutoff 1000) showed a growth-stimulating activity in a dose-dependent manner on B12 assay medium with ammonium sulfate. One of the growth stimulators was purified from the retained fraction by acetone precipitation, solid-phase extraction with a hydrophobic pretreatment column, and multistage reversed-phase HPLC. An increase of 53-fold in the specific activity, and a recovery of 1.3% were obtained. The amino acid composition and N-terminal sequence analysis of this growth stimulator provided the structure of Ala-Thr-Pro-Glu-Lys-Glu-Glu-Pro-Thr-Ala. The molecular mass was 1075 by MALDI-TOF MS analysis. These results showed that this growth stimulator was a decapeptide with the sequence shown above. This is the first report that clarified the structure of an active peptide for the growth of Bifidobacterium.
Etoh
S
S
Division of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, Japan.
Asamura
K
K
Obu
A
A
Sonomoto
K
K
Ishizaki
A
A
D000595:Amino Acid Sequence; D001644:Bifidobacterium / Q000737:chemistry*; D002850:Chromatography, Gel; D006133:Growth Substances / Q000737:chemistry / Q000302:isolation & purification*; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification*; D012408:Rubber / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D006133:Growth Substances; D010455:Peptides; D012408:Rubber