Use of large-scale expression cloning screens in the Xenopus laevis tadpole to identify gene function.
We have conducted an expression cloning screen of approximately 50, 000 cDNAs from a tadpole stage Xenopus laevis cDNA library to functionally identify genes affecting a wide range of cellular and developmental processes. Fifty-seven cDNAs were isolated for their ability to alter gross tadpole morphology or the expression patterns of tissue-specific markers. Thirty-seven of the cDNAs have not been previously described for Xenopus, and 15 of these show little or no similarity to sequences in the NCBI database. The screen and the identified genes are presented in this paper to demonstrate the power, ease, speed, and flexibility of expression cloning in the X. laevis embryo. Future screens such as this one can be done on a larger scale and will complement the sequence-based screens and genome-sequencing projects which are producing a large body of novel genes without ascribed functions.
Grammer
T C
TC
Department of Molecular and Cell Biology, University of California at Berkeley, 401 Barker Hall, Berkeley, California 94720, USA.
Liu
K J
KJ
Mariani
F V
FV
Harland
R M
RM
D000818:Animals; D017209:Apoptosis; D019521:Body Patterning; D003001:Cloning, Molecular; D018076:DNA, Complementary; D019992:Databases as Topic; D004625:Embryo, Nonmammalian / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D008675:Metamorphosis, Biological; D010858:Pigmentation / Q000235:genetics; D014982:Xenopus laevis / Q000196:embryology / Q000235:genetics* / Q000254:growth & development
D018076:DNA, Complementary