title: Interleukin-5-mediated allergic airway inflammation inhibits the human surfactant protein C promoter in transgenic mice.
abstract: Allergen challenge in the lung of humans and animals is associated with surfactant dysfunction, but the mechanism of this effect has not been established. By using a murine model of asthma we now report the effect of allergen-induced airway inflammation on the expression of transgenes regulated by the human surfactant protein (hSP)-C promoter. The hSP-C 3.7-kilobase pair promoter was used to direct the expression of eotaxin, an eosinophil-selective chemokine, into the lungs of several transgenic lines. As expected, the transgenic mice expressed increased amounts of eotaxin mRNA and protein compared with wild-type mice. Surprisingly, following allergen challenge, there was a marked down-regulation of transgene mRNA in three independent transgenic lines. The down-regulation was in contrast to other related proteins such as endogenous eotaxin and surfactant protein D levels, which were both increased following allergen challenge. Consistent with specific down-regulation of the eotaxin transgene, there was no increase in pulmonary eosinophil levels in the transgenic mice above that found in wild-type mice. Analysis of hSP-C transgenic mice with distinct reporter genes and 3'-untranslated regions revealed that allergen challenge was directly affecting the hSP-C promoter. We hypothesized that allergen-induced down-regulation of the hSP-C promoter was related to the eosinophilic inflammation. To test this, we blocked eosinophilic inflammation in the lungs by treating mice with neutralizing antiserum against interleukin-5. Interestingly, this treatment also blocked allergen-induced inhibition of the hSP-C promoter. These results establish that allergic airway inflammation is associated with up-regulation of the surfactant proteins primarily involved in immunity, whereas down-regulation of the surfactant protein primarily involved in maintaining airway patency. Furthermore, the marked down-regulation of the hSP-C promoter is interleukin-5-dependent, implying a critical role for eosinophilic inflammation. These results suggest that alterations in surfactant protein levels may contribute to immune and airway dysfunction in asthma.
authors: Division of Pulmonary Medicine, Allergy and Clinical Immunology, Department of Pediatrics, Children's Hospital Medical Center, Cincinnati, Ohio 45229, USA.
authors: A
authors: A
authors: Mishra
authors: T E
authors: TE
authors: Weaver
authors: D C
authors: DC
authors: Beck
authors: M E
authors: ME
authors: Rothenberg
mesh_terms: D020413:3' Untranslated Regions / Q000502:physiology; D000818:Animals; D001249:Asthma / Q000209:etiology / Q000378:metabolism*; D054413:Chemokine CCL11; D019742:Chemokines, CC; D016207:Cytokines / Q000502:physiology*; D015536:Down-Regulation; D015848:Interleukin-5 / Q000502:physiology*; D051379:Mice; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic; D011510:Proteolipids / Q000235:genetics* / Q000502:physiology; D011663:Pulmonary Surfactants / Q000235:genetics* / Q000502:physiology; D019076:Transgenes
chemical_list: D020413:3' Untranslated Regions; C515456:CCL11 protein, human; C515457:Ccl11 protein, mouse; D054413:Chemokine CCL11; D019742:Chemokines, CC; D016207:Cytokines; D015848:Interleukin-5; D011510:Proteolipids; D011663:Pulmonary Surfactants