Characterization of phage receptors in Streptococcus thermophilus using purified cell walls obtained by a simple protocol.
A simple protocol was designed and applied to obtain Streptococcus thermophilus purified cell walls. To identify the structures involved in phage adsorption, the cell walls of two Strep. thermophilus strains were treated with sodium dodecyl sulphate and proteinase K. These treatments did not reduce the adsorption of phages CYM and 0BJ to the cell walls of Strep. thermophilus YSD10 and Strep. thermophilus BJ15, respectively. However, phage binding was reduced when the cell envelopes were treated with mutanolysin or trichloroacetic acid 5%, suggesting that the phage receptor component is part of the peptidoglycan or a polymer closely linked to it. The ability of several saccharides to inactivate both phages was also assayed. These phage inhibition experiments suggested that the phage CYM adsorbed to a component involving glucosamine and rhamnose, while glucosamine and ribose interfered with the adsorption of phage 0BJ.
Quiberoni
A
A
Programa de Lactología Industrial, Facultad de Ingeniería Química (Universidad Nacional del Litoral), Santa Fe, Argentina.
Stiefel
J I
JI
Reinheimer
J A
JA
D000327:Adsorption; D002241:Carbohydrates / Q000494:pharmacology; D002473:Cell Wall / Q000378:metabolism; D019286:Endopeptidase K / Q000378:metabolism; D010450:Endopeptidases / Q000494:pharmacology; D005944:Glucosamine / Q000494:pharmacology; D011991:Receptors, Virus / Q000378:metabolism*; D012210:Rhamnose / Q000494:pharmacology; D012266:Ribose / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013291:Streptococcus / Q000378:metabolism*; D017108:Streptococcus Phages / Q000378:metabolism*; D014238:Trichloroacetic Acid / Q000494:pharmacology
D002241:Carbohydrates; D011991:Receptors, Virus; D012967:Sodium Dodecyl Sulfate; D014238:Trichloroacetic Acid; D012266:Ribose; D010450:Endopeptidases; D019286:Endopeptidase K; C021816:mutanolysin; D005944:Glucosamine; D012210:Rhamnose