title: The biochemical profile of rat testicular tissue as measured by magic angle spinning 1H NMR spectroscopy.
abstract: The testis is the principal organ of male fertility, responsible for the production of spermatozoa and their maturation into sperm. However, the underlying biochemistry of the testis is relatively understudied. The fluidic and homogeneous nature of the testis makes it an ideal organ for high resolution magic angle spinning (MAS) 1H NMR spectroscopy. In this study we have catalogued the low molecular weight metabolites. The testis contains large amounts of creatine, of which a substantial proportion was shown to be extracellular using bipolar gradients to measure apparent diffusion coefficients. The tissue also contained relatively high amounts of uridine.
authors: Biological Chemistry, Biomedical Sciences Division, Imperial College of Science, Technology and Medicine, South Kensington, UK. j.griffin@ic.ac.uk
authors: J L
authors: JL
authors: Griffin
authors: J
authors: J
authors: Troke
authors: L A
authors: LA
authors: Walker
authors: R F
authors: RF
authors: Shore
authors: J C
authors: JC
authors: Lindon
authors: J K
authors: JK
authors: Nicholson
mesh_terms: D000409:Alanine / Q000032:analysis; D000818:Animals; D002794:Choline / Q000032:analysis; D003401:Creatine / Q000032:analysis; D004058:Diffusion; D005110:Extracellular Space / Q000737:chemistry; D007424:Intracellular Fluid / Q000737:chemistry; D019344:Lactic Acid / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D010713:Phosphatidylcholines / Q000032:analysis; D051381:Rats; D013737:Testis / Q000737:chemistry*; D014529:Uridine / Q000032:analysis; D014867:Water / Q000032:analysis
chemical_list: D010713:Phosphatidylcholines; D014867:Water; D019344:Lactic Acid; D003401:Creatine; D002794:Choline; D000409:Alanine; D014529:Uridine