title: Engineering aspects of enzymatic signal transduction: photoreceptors in the retina.
abstract: Identifying the basic module of enzymatic amplification as an irreversible cycle of messenger activation/deactivation by a "push-pull" pair of opposing enzymes, we analyze it in terms of gain, bandwidth, noise, and power consumption. The enzymatic signal transduction cascade is viewed as an information channel, the design of which is governed by the statistical properties of the input and the noise and dynamic range constraints of the output. With the example of vertebrate phototransduction cascade we demonstrate that all of the relevant engineering parameters are controlled by enzyme concentrations and, from functional considerations, derive bounds on the required protein numbers. Conversely, the ability of enzymatic networks to change their response characteristics by varying only the abundance of different enzymes illustrates how functional diversity may be built from nearly conserved molecular components.
authors: Department of Physiology and Biophysics, University of Washington, Seattle, Washington 98195, USA.
authors: P B
authors: PB
authors: Detwiler
authors: S
authors: S
authors: Ramanathan
authors: A
authors: A
authors: Sengupta
authors: B I
authors: BI
authors: Shraiman
mesh_terms: D000818:Animals; D001703:Biophysics / Q000379:methods; D002118:Calcium / Q000502:physiology; D004798:Enzymes / Q000378:metabolism*; D005246:Feedback; D006153:Guanosine Diphosphate / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D008954:Models, Biological; D020419:Photoreceptor Cells, Vertebrate / Q000502:physiology*; D012160:Retina / Q000502:physiology*; D012243:Rhodopsin / Q000378:metabolism; D012374:Rod Cell Outer Segment / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology*; D015293:Transducin / Q000378:metabolism; D014714:Vertebrates; D014785:Vision, Ocular / Q000502:physiology*
chemical_list: D004798:Enzymes; D006153:Guanosine Diphosphate; D006160:Guanosine Triphosphate; D012243:Rhodopsin; D015293:Transducin; D002118:Calcium