Sulfated glycosaminoglycans from ovary of Rhodnius prolixus.
We have characterized sulfated glycosaminoglycans from ovaries of the blood-sucking insect Rhodnius prolixus, and determined parameters of their synthesis and distribution within this organ by biochemical and histochemical procedures. The major sulfated glycosaminoglycan is heparan sulfate while chondroitin 4-sulfate is a minor component. These glycosaminoglycans are concentrated in the ovarian tissue and are not found inside the oocytes. Besides this, we detected the presence of a sulfated compound distinguished from sulfated glycosaminoglycans and possibly derived from sulfated proteins. Conversely to the compartmental location of sulfated glycosaminoglycans, the unidentified sulfated compound is located in the ovarian tissue as well as inside the oocytes. Based on these and other findings, the possible roles of ovarian sulfated glycosaminoglycans on the process of oogenesis in these insects are discussed.
Costa-Filho
A
A
Laboratório de Tecido Conjuntivo, Hospital Universitário Clementino Fraga Filho, Rio de Janeiro, Brazil.
Werneck
C C
CC
Nasciutti
L E
LE
Masuda
H
H
Atella
G C
GC
Silva
L F
LF
D000818:Animals; D002809:Chondroitin Sulfates / Q000378:metabolism; D005260:Female; D006025:Glycosaminoglycans / Q000378:metabolism*; D006497:Heparan Sulfate / Q000378:metabolism; D007553:Isotope Labeling; D008297:Male; D010053:Ovary / Q000378:metabolism; D012239:Rhodnius / Q000378:metabolism*; D013194:Staining and Labeling / Q000379:methods; D013462:Sulfur Radioisotopes
D002809:Chondroitin Sulfates; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D013462:Sulfur Radioisotopes; C013786:A73025