Differential expression in an antisense MYCN neuroblastoma model.
BACKGROUND
A previously published antisense MYCN-expressing model system was utilized to identify genes whose expression is altered by the down-regulation of MYCN by AS MYCN.


RESULTS
Differential display comparing nontransfected human NB NBL-S cells to three AS MYCN-expressing cell lines (NBAS-4, -5, and -6) yielded nine differentially expressed cDNAs designated NDDE:1-9, for MYCN-dependent differential expression genes. A GenBank search revealed matches for seven of the nine cDNAs. Differential expression was confirmed for five of the cDNAs by Northern blot analysis.


RESULTS
NDDE:8 is up-regulated in the AS MYCN-expressing clones and shares homology with the EB1 clone p53-induced gene (PRG3). NDDE:2 is up-regulated in the high-expressing N-myc protein NBL-S cells and shares homology with a 27 kD heat shock protein PAN1 clone. Further analysis of all five cDNAs might further elucidate the targets of MYCN in NB.
Schmidt
M L
ML
Department of Pediatrics, University of Illinois at Chicago, Chicago, Illinois, USA. mls3@uic.edu
Lal
A
A
Dittmann
D
D
D016375:Antisense Elements (Genetics); D002648:Child; D018076:DNA, Complementary / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D016259:Genes, myc / Q000235:genetics*; D006801:Humans; D009447:Neuroblastoma / Q000235:genetics*; D014407:Tumor Cells, Cultured
D016375:Antisense Elements (Genetics); D018076:DNA, Complementary