A comparison of human immunodeficiency virus type-1 protease inhibition activities by the aqueous and methanol extracts of Chinese medicinal herbs.
The aqueous and methanol extracts of thirty-one herbs traditionally used as anti-fever remedies in China were screened for their in vitro inhibition on human immunodeficiency virus type-1 protease (HIV-1 PR). The activity of recombinant HIV-1 protease was determined by sequence-specific cleavage at the Tyr-Pro bond of the fluorogenic substrate (Arg-Glu(EDANS)-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-Lys(DABCYL)- Arg) or by HPLC anaylsis of the cleavage products after incubation of the enzyme with a synthetic peptide substrate (Acetyl-Ser-Gln-Asn-Tyr-Pro-Val-Val-amide). Among the herbal extracts examined, the aqueous extracts of Prunella vulgaris and Scutellaria baicalensis and the methanol extracts of Woodwardia unigemmata, Paeonica suffruticosa and Spatholobus suberectus elicited significant inhibition (>90%) at a concentration of 200 microg/ml.
Lam
T L
TL
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, New Territories.
Lam
M L
ML
Au
T K
TK
Ip
D T
DT
Ng
T B
TB
Fong
W P
WP
Wan
D C
DC
D016333:HIV Protease / Q000378:metabolism*; D017320:HIV Protease Inhibitors / Q000494:pharmacology*; D015497:HIV-1 / Q000201:enzymology; D006801:Humans; D007700:Kinetics; D019684:Magnoliopsida; D008516:Medicine, Chinese Traditional; D000432:Methanol; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors; D014867:Water
D017320:HIV Protease Inhibitors; D010936:Plant Extracts; D011994:Recombinant Proteins; D014867:Water; D016333:HIV Protease; D000432:Methanol