The study on adriamycin induced DNA scissions in human cells with different activity of some antioxidant enzymes.
Although the anthracycline antibiotic adriamycin has been widely used in treatment of variety of human malignances, the mechanism of its biological activity is still unknown. One of the proposed mechanisms of action assumes the generation of active oxygen species which may damage DNA of cells. In this study we investigated if the occurrence of DNA breaks induced by adriamycin depends on the intracellular level of antioxidant enzymes. The study was performed with two human cell lines (CRL2088 and CLV102) differing in activity of catalase and superoxide dismutase. The results indicated that the level of DNA breaks in both lines treated with adriamycin was similar despite of differences in the enzymes activity.
Chłopkiewicz
B
B
Drug Institute, 30/34 Chełmska Str., 00-725 Warsaw, Poland.
Gruber
B
B
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000032:analysis / Q000494:pharmacology; D002460:Cell Line; D004249:DNA Damage / Q000187:drug effects*; D004317:Doxorubicin / Q000494:pharmacology*; D006801:Humans; D009152:Mutagenicity Tests; D013482:Superoxide Dismutase / Q000032:analysis / Q000494:pharmacology
D000903:Antibiotics, Antineoplastic; D000975:Antioxidants; D004317:Doxorubicin; D002374:Catalase; D013482:Superoxide Dismutase