title: Detection of exogenous intake of natural corticosteroids by gas chromatography/combustion/isotope ratio mass spectrometry: application to misuse in sport.
abstract: A detailed procedure for the analysis of exogenous hydrocortisone and cortisone in urine by gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS) is proposed. As urinary levels of hydrocortisone are rather low for GC/C/IRMS analysis, the focus is on the main corticosteroid metabolites, tetrahydrocortisone (THE) and tetrahydrocortisol (THF). Following different solid phase extraction purifications, THE and THF are oxidized to 5beta-androstanetrione before analysis by GC/C/IRMS. Significant differences in delta(13)C per thousand values of synthetic natural corticosteroids and endogenous human corticosteroids have been observed. Therefore, a positive criterion, to detect exogenous administration of synthetic corticosteroids in anti-doping control, is proposed.
authors: Laboratoire National de Dépistage du Dopage, 143 avenue R. Salengro, 92290 Chatenay Malabry, France.
authors: E
authors: E
authors: Bourgogne
authors: V
authors: V
authors: Herrou
authors: J C
authors: JC
authors: Mathurin
authors: M
authors: M
authors: Becchi
authors: J
authors: J
authors: de Ceaurriz
mesh_terms: D000305:Adrenal Cortex Hormones / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism / Q000652:urine*; D000328:Adult; D000731:Androstanes / Q000737:chemistry / Q000652:urine; D003348:Cortisone / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism / Q000652:urine; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D006854:Hydrocortisone / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism / Q000652:urine; D008297:Male; D008875:Middle Aged; D015394:Molecular Structure; D013177:Sports; D013760:Tetrahydrocortisol / Q000737:chemistry / Q000652:urine; D013761:Tetrahydrocortisone / Q000737:chemistry / Q000652:urine; D013997:Time Factors
chemical_list: D000305:Adrenal Cortex Hormones; D000731:Androstanes; D013761:Tetrahydrocortisone; D013760:Tetrahydrocortisol; D003348:Cortisone; D006854:Hydrocortisone