Pentobarbital-activated Cl(-) channels in cultured adult and embryonic human DRG neurons.
Developmental differences in pentobarbital-activated Cl(-) currents were studied in adult and embryonic human dorsal root ganglia (DRG) neurons using whole-cell patch-clamp recordings. Pentobarbital-induced Cl(-) conductance was significantly greater in adult DRGs (28.4 pS) than in embryonic cells (19.1 pS). Fluctuation analysis of the spectral density plots of Cl(-) channel activation by pentobarbital showed age differences in the length and number of open time constants (adult cells, tau(1), tau(2), tau(3) were 224, 8. 4, 1.5 ms, respectively; embryonic cells tau(1) and tau (2) were 165 and 26.3 ms, respectively). The different kinetic properties of human adult and embryonic DRG Cl(-) channels opened by pentobarbital may reflect the presence of different subunits in the two populations of neurons.
Valeyev
A Y
AY
Neurophysiology and Spinal Cord Pharmacology Laboratories, Veterans Affairs Medical Center, Miami, FL 33101, USA. avaleyev@med.miami.edu
Hackman
J C
JC
Holohean
A M
AM
Wood
P M
PM
Davidoff
R A
RA
D002478:Cells, Cultured; D018118:Chloride Channels / Q000187:drug effects / Q000502:physiology*; D004553:Electric Conductivity; D005333:Fetus / Q000502:physiology; D005727:Ganglia, Spinal / Q000166:cytology / Q000196:embryology* / Q000502:physiology*; D006801:Humans; D009474:Neurons / Q000502:physiology*; D018408:Patch-Clamp Techniques; D010424:Pentobarbital / Q000494:pharmacology*
D018118:Chloride Channels; D010424:Pentobarbital