title: Regulation of glycolysis during acclimation of scallops (Patinopecten yessoensis Jay) to anaerobiosis.
abstract: Some glycolytic metabolites in the adductor muscle were measured after transfer of scallops from aerobic to anaerobic saltwater for 12 h. The level of octopine increased gradually during the initial 3 h incubation, and thereafter the level increased rapidly up to 12 h. The ATP level also did not show any significant change for the initial 3 h, and then decreased rapidly. The fructose 2,6-biphosphate (Fru 2,6-BP) level increased drastically during the initial 3 h incubation, but thereafter the level did not show any significant change up to 12 h. In the short-term effects of anaerobiosis for 90 min, the level of fructose 6-phosphate (Fru 6-P) increased just after transfer to anaerobiosis, and then its level decreased. In contrast, the fructose 1,6-biphosphate (Fru 1,6-BP) level increased greatly, at the time when both glucose 6-phosphate (Glc 6-P) and Fru 6-P decreased. The Fru 2,6-BP level did not any significant change during the initial 15 min incubation, but thereafter the level increased gradually up to 90 min. Scallop 6-phosphofructo 1-kinase (EC 2.7.1.11) (PFK1) was strongly activated by 1 microM Fru 2,6-BP when 0.2 mM Fru 6-P was used as a substrate, but the activity was not affected at 5 mM Fru 6-P. In view of these results, the regulation mechanism of glycolysis is discussed.
authors: Department of Food Science, Ishikawa Agricultural College, Nonoichi, Japan. enomoto@ishikawa-c.ac.jp
authors: T
authors: T
authors: Enomoto
authors: C
authors: C
authors: Nakao
authors: H
authors: H
authors: Ohyama
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000378:metabolism; D005285:Fermentation; D005635:Fructosediphosphates / Q000378:metabolism; D005636:Fructosephosphates / Q000378:metabolism; D019298:Glucose-6-Phosphate / Q000378:metabolism; D006019:Glycolysis; D000860:Hypoxia; D008974:Mollusca / Q000378:metabolism* / Q000502:physiology*; D010100:Oxygen / Q000378:metabolism*; D010101:Oxygen Consumption; D010732:Phosphofructokinase-1 / Q000378:metabolism; D013997:Time Factors
chemical_list: D005635:Fructosediphosphates; D005636:Fructosephosphates; C005394:octopine; D019298:Glucose-6-Phosphate; C027618:fructose-6-phosphate; C027652:fructose 2,6-diphosphate; D000255:Adenosine Triphosphate; D001120:Arginine; D010732:Phosphofructokinase-1; C029063:fructose-1,6-diphosphate; D010100:Oxygen