Solid-phase synthesis of the glycopeptide of human glycophorin AM bearing the consecutive sialyl-T antigen.
Fmoc solid-phase synthesis of the N-terminal glycopentapeptide of human glycophorin AM, bearing the consecutive sialyl-T antigen, was accomplished using glycosylated amino acid building blocks on a weakly acid-labile resin with high efficiency. The benzylated glycopeptide was treated with TMSOTf-thioanisole in TFA and then with aq NaHCO3 to afford the deprotected glycopeptide in good yield.
Ando
S
S
Institute of Physical and Chemical Research (RIKEN), Saitama, Japan.
Nakahara
Y
Y
Ito
Y
Y
Ogawa
T
T
Nakahara
Y
Y
D000596:Amino Acids / Q000737:chemistry; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000737:chemistry*; D002240:Carbohydrate Sequence; D006020:Glycopeptides / Q000138:chemical synthesis* / Q000737:chemistry* / Q000276:immunology; D006021:Glycophorins / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D008969:Molecular Sequence Data; D015394:Molecular Structure
D000596:Amino Acids; D015295:Antigens, Tumor-Associated, Carbohydrate; D006020:Glycopeptides; D006021:Glycophorins; C055491:sialosyl-Tn antigen