title: Thermally induced conformational changes in horseradish peroxidase.
abstract: Detailed differential scanning calorimetry (DSC), steady-state tryptophan fluorescence and far-UV and visible CD studies, together with enzymatic assays, were carried out to monitor the thermal denaturation of horseradish peroxidase isoenzyme c (HRPc) at pH 3.0. The spectral parameters were complementary to the highly sensitive but integral method of DSC. Thus, changes in far-UV CD corresponded to changes in the overall secondary structure of the enzyme, while that in the Soret region, as well as changes in intrinsic tryptophan fluorescence emission, corresponded to changes in the tertiary structure of the enzyme. The results, supported by data about changes in enzymatic activity with temperature, show that thermally induced transitions for peroxidase are irreversible and strongly dependent upon the scan rate, suggesting that denaturation is under kinetic control. It is shown that the process of HRPc denaturation can be interpreted with sufficient accuracy in terms of the simple kinetic scheme N -->k D where k is a first-order kinetic constant that changes with temperature, as given by the Arrhenius equation; N is the native state, and D is the denatured state. On the basis of this model, the parameters of the Arrhenius equation were calculated.
authors: Departamento de Bioquímica y Biología Molecular, Universidad de Salamanca, Spain.
authors: D G
authors: DG
authors: Pina
authors: A V
authors: AV
authors: Shnyrova
authors: F
authors: F
authors: Gavilanes
authors: A
authors: A
authors: Rodríguez
authors: F
authors: F
authors: Leal
authors: M G
authors: MG
authors: Roig
authors: I Y
authors: IY
authors: Sakharov
authors: G G
authors: GG
authors: Zhadan
authors: E
authors: E
authors: Villar
authors: V L
authors: VL
authors: Shnyrov
mesh_terms: D002151:Calorimetry; D002942:Circular Dichroism; D006735:Horseradish Peroxidase / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D011487:Protein Conformation; D011489:Protein Denaturation; D013050:Spectrometry, Fluorescence; D013696:Temperature
chemical_list: D006735:Horseradish Peroxidase