title: Coexpression of granulocyte colony stimulating factor and its receptor in primary ovarian carcinomas.
abstract: Immunohistochemistry was used to determine the expression of granulocyte colony-stimulating factor (G-CSF) and its receptor (G-CSFR) in primary ovarian carcinomas. The expression of G-CSFR was observed in the malignant cells of each of the 46 primary carcinomas examined; G-CSF was coexpressed in both the malignant epithelial cells and the stroma of 56.5% of the specimens. Thus the majority of ovarian carcinomas harbor both potential autocrine and paracrine G-CSF axes. In 37% of the samples, G-CSF was expressed only within stromal cells, suggesting that only a potential paracrine system is in place. In a preliminary, retrospective, evaluation, the survival of patients whose tumors expressed only the apparent paracrine loop was significantly worse than patients whose tumors expressed both potential autocrine and paracrine G-CSF-based regulatory loops (14.5 vs. 42.5 months, respectively). Studies on the potential function of G-CSF were performed using the G-CSFR-expressing OVCAR-3 ovarian carcinoma line. As a single agent, rhG-CSF failed to stimulate [3H]-thymidine incorporation in these cells, but enhanced the mitogenic action of epidermal growth factor (EGF) in a dose-dependent manner. Thus, potential autocrine and/or paracrine loops involving G-CSF and its receptor occur in over 90% of primary ovarian carcinomas, and may act to modulate the action of growth factors.
authors: Cytokine/Cytokine Receptor Laboratory, LINK Laboratories, UMass Cancer Center, University of Massachusetts Medical School, Worcester, MA 01655, USA. todd.savarese@umassmed.edu
authors: T M
authors: TM
authors: Savarese
authors: K
authors: K
authors: Mitchell
authors: C
authors: C
authors: McQuain
authors: C L
authors: CL
authors: Campbell
authors: R
authors: R
authors: Guardiani
authors: J
authors: J
authors: Wuu
authors: C
authors: C
authors: Ollari
authors: F
authors: F
authors: Reale
authors: B E
authors: BE
authors: Nelson
authors: A
authors: A
authors: Chen
authors: P J
authors: PJ
authors: Quesenberry
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000032:analysis* / Q000494:pharmacology; D006801:Humans; D007150:Immunohistochemistry; D010051:Ovarian Neoplasms / Q000737:chemistry* / Q000473:pathology; D016188:Receptors, Granulocyte Colony-Stimulating Factor / Q000032:analysis*; D011994:Recombinant Proteins; D013936:Thymidine / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D016188:Receptors, Granulocyte Colony-Stimulating Factor; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D004815:Epidermal Growth Factor; D013936:Thymidine