The mechanism of apoptosis in human platelets during storage.
BACKGROUND
Although it is usually involved only in nucleated cells (NCs), artificially enucleated cells also lose viability by a programmed process of cell death called apoptosis. Because platelets undergo loss of viability during storage, an attempt was made to determine whether platelets contained the apoptotic mechanisms and whether it was activated during platelet storage.


METHODS
Platelet viability was measured by reduction of a tetrazolium dye (MTS) and annexin V binding. Members of the death receptor, caspase, and Bcl-2 families were detected by RNase protection assay and Western blotting. Caspase 3 activation was measured by enzyme and Western blot assays and by cleavage of gelsolin.


RESULTS
After 5 days of storage under standard blood banking conditions, platelets display biochemical signs of apoptosis by losing MTS activity and increasing the amount of phosphatidylserine on their surface. The mRNA and the proenzyme for several members of the caspase, death receptor, and Bcl-2 families are expressed at high levels in platelets. An increase in caspase 3 activity and the amount of the biologically active p17 subunit of active caspase 3 were observed to coincide with the appearance of apoptotic markers during storage. These effects were not due to platelet activation. The caspase 3 substrate, gelsolin, began to undergo proteolysis after 3 to 4 days of storage, and the addition of the caspase inhibitor z-VAD-fmt substantially inhibited this process.


CONCLUSIONS
Platelets contain many of the components of the apoptotic mechanism and show activation of caspase 3 and consequent cleavage of gelsolin during storage, independent of platelet activation. Evaluation of the mechanism of apoptosis in platelets may provide a basis for developing novel strategies to enhance platelet viability during storage.
Li
J
J
Hematology Oncology Unit, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA.
Xia
Y
Y
Bertino
A M
AM
Coburn
J P
JP
Kuter
D J
DJ
D017209:Apoptosis / Q000502:physiology*; D001792:Blood Platelets / Q000166:cytology* / Q000201:enzymology; D001793:Blood Preservation; D001798:Blood Proteins / Q000378:metabolism; D053148:Caspase 3; D020169:Caspases / Q000097:blood / Q000378:metabolism; D002470:Cell Survival / Q000502:physiology; D004789:Enzyme Activation / Q000502:physiology; D018260:Gelsolin / Q000378:metabolism; D006801:Humans; D011509:Proteoglycans
D001798:Blood Proteins; D018260:Gelsolin; D011509:Proteoglycans; C042057:proteolysis-inducing peptide; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases