Stimulatory action of Pluchea quitoc extract on the hematopoietic response during murine listeriosis.
The importance of both granulocytes and macrophages in the response to Listeria monocytogenes infection make this infection a suitable choice to investigate the effects of Pluchea quitoc on hematopoiesis. A significant depletion of bone marrow granulocyte-macrophage progenitor cells (CFU-GM) was observed at 48 and 72 h after intraperitoneal infection of mice with 1 x 10(4) L. monocytogenes. However, the treatment of infected animals with P. quitoc ethanolic extract (250, 500 or 1000 mg/kg) given orally for 3 consecutive days prior to infection produced a stimulatory effect on myelopoiesis, restoring the number of CFU-GM to normal. This same dose-schedule also increased colony formation in normal mice as compared to controls. In addition, P. quitoc significantly enhanced survival of infected mice. Thus, it is probable that the ability of P. quitoc to induce a higher reserve of granulocyte-macrophage precursors in the bone marrow is of major significance in determining early resistance to infection.
Queiroz
M L
ML
Department of Pharmacology and Hemocentre, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, SP, Brazil. msq44@uol.com.br
Justo
G Z
GZ
Pereira-da-Silva
F R
FR
Müller
A H
AH
Guilhon
G M
GM
D000818:Animals; D003114:Colony-Forming Units Assay; D006098:Granulocytes / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D006410:Hematopoiesis / Q000187:drug effects*; D008088:Listeriosis / Q000188:drug therapy* / Q000276:immunology* / Q000473:pathology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal
D010936:Plant Extracts