Efficient transformation of Dictyostelium discoideum with a particle inflow gun.
We report experiments to transform Dictyostelium discoideum using a simple home-made particle gun. Stable transformants were obtained at frequencies of up to 2500 clones/microg DNA. This is five times more than we achieve with the same vector using electroporation protocols. We also show that the particle inflow gun can be used for analysis of developmentally regulated gene expression in a transient assay.
Wetterauer
B
B
Zoologisches Institut der Universität München, Munich, Germany. wetterau@zi.biologie.uni-muenchen.de
Salger
K
K
Demel
P
P
Koop
H
H
D000818:Animals; D019470:Biolistics / Q000295:instrumentation*; D002472:Cell Transformation, Viral; D004023:Dictyostelium / Q000235:genetics*; D005822:Genetic Vectors; D006046:Gold; D010316:Particle Size; D014414:Tungsten; D001616:beta-Galactosidase / Q000235:genetics
D006046:Gold; D001616:beta-Galactosidase; D014414:Tungsten