Tyrosine hydroxylase-based DNA-vaccination is effective against murine neuroblastoma.
BACKGROUND
The disruption of self-tolerance against neuroblastoma is the ultimate goal of an effective DNA-vaccine.


METHODS
Here we demonstrate the induction of a protective immunity against syngeneic murine NXS2 neuroblastoma in A/J mice, following vaccination with tyrosine hydroxylase (TH) derived antigens. Oral gene delivery was accomplished using an attenuated strain of Salmonella typhimurium as a carrier harboring vectors encoding for mTH antigens.


RESULTS
Vaccination was effective in protecting animals from a lethal challenge with wild-type NXS2 tumor cells.


CONCLUSIONS
These results provide the first evidence of the TH self antigen being recognized by T-cells and demonstrate that a TH-based DNA vaccine is a potentially useful immunotherapeutic strategy for neuroblastoma.
Lode
H N
HN
The Scripps Research Institute, Department of Immunology, La Jolla, California, USA. holger.lode@charite.de
Pertl
U
U
Xiang
R
R
Gaedicke
G
G
Reisfeld
R A
RA
D000818:Animals; D018014:Gene Transfer Techniques; D051379:Mice; D009447:Neuroblastoma / Q000276:immunology / Q000517:prevention & control*; D010957:Plasmids; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics; D019444:Vaccines, DNA
D019444:Vaccines, DNA; D014446:Tyrosine 3-Monooxygenase