Effects of homocysteine on the binding of extracellular-superoxide dismutase to the endothelial cell surface.
Homocysteine is known to be a risk factor for several vascular diseases. Previously, we found a significant association between plasma homocysteine and plasma extracellular-superoxide dismutase (EC-SOD) levels. The binding of EC-SOD to human and bovine aortic endothelial cell cultures showed significant decreases after incubation with 10 microM homocysteine, whereas the expression of EC-SOD in fibroblast cell cultures was inhibited with a high concentration (1 mM) of homocysteine. Furthermore, binding of EC-SOD to heparin immobilized on plates was decreased with homocysteine. These observations suggested that homocysteine decreases the binding of EC-SOD to vascular endothelial cell surfaces by degradation of endothelial heparan sulfate proteoglycan, which results in a loss of the ability to protect endothelial cell surfaces from oxidative stress.
Yamamoto
M
M
Laboratory of Clinical Pharmaceutics, Gifu Pharmaceutical University, 5-6-1 Mitahora-higashi, 502-8585, Gifu, Japan.
Hara
H
H
Adachi
T
T
D000818:Animals; D001011:Aorta / Q000166:cytology; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006493:Heparin / Q000378:metabolism; D006710:Homocysteine / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D012867:Skin / Q000166:cytology; D013482:Superoxide Dismutase / Q000378:metabolism*
D006710:Homocysteine; D006493:Heparin; D013482:Superoxide Dismutase