title: Programmed cell death in the developing somites is promoted by nerve growth factor via its p75(NTR) receptor.
abstract: Neurotrophins control neuron number during development by promoting the generation and survival of neurons and by regulating programmed neuronal death. In the latter case, the cell death induced by nerve growth factor (NGF) in the developing chick retina is mediated by p75(NTR), the common neurotrophin receptor (J. M. Frade, A. Rodriguez-Tebar, and Y.-A. Barde, 1996, Nature 383, 166-168). Here we show that NGF also induces the programmed death of paraxial mesoderm cells in the developing somites. Both NGF and p75(NTR) are expressed in the somites of chick embryos at the time and the place of programmed cell death. Moreover, neutralizing the activity of endogenous NGF with a specific blocking antibody, or antagonizing NGF binding to p75(NTR) by the application of human NT-4/5, reduces the levels of apoptotic cell death in both the sclerotome and the dermamyotome by about 50 and 70%, respectively. Previous data have shown that Sonic hedgehog is necessary for the survival of differentiated somite cells. Consistent with this, Sonic hedgehog induces a decrease of NGF mRNA in somite explant cultures, thus showing the antagonistic effect of NGF and Sonic hedgehog with respect to somite cell survival. The regulation of programmed cell death by NGF/p75(NTR) in a mesoderm-derived tissue demonstrates the capacity of neurotrophins and their receptors to influence critical developmental processes both within and outside of the nervous system.
authors: Instituto Cajal de Neurobiología, CSIC, Avenida Doctor Arce, 37, E-28002 Madrid, Spain.
authors: M L
authors: ML
authors: Cotrina
authors: M
authors: M
authors: González-Hoyuela
authors: J A
authors: JA
authors: Barbas
authors: A
authors: A
authors: Rodríguez-Tébar
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D002642:Chick Embryo; D004475:Ectoderm / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D005314:Embryonic and Fetal Development; D006801:Humans; D008648:Mesoderm / Q000166:cytology / Q000502:physiology; D020932:Nerve Growth Factor / Q000502:physiology*; D009414:Nerve Growth Factors / Q000494:pharmacology; D009420:Nervous System / Q000166:cytology / Q000187:drug effects / Q000196:embryology; D018696:Neuroprotective Agents / Q000494:pharmacology; D009928:Organ Specificity; D020800:Receptor, Nerve Growth Factor; D020917:Receptor, trkA / Q000502:physiology; D017475:Receptors, Nerve Growth Factor / Q000502:physiology*
chemical_list: D009414:Nerve Growth Factors; D018696:Neuroprotective Agents; D020800:Receptor, Nerve Growth Factor; D017475:Receptors, Nerve Growth Factor; C071951:neurotrophin 5; D020932:Nerve Growth Factor; D020917:Receptor, trkA; C073922:neurotrophin 4