Pentapeptide scanning mutagenesis: encouraging old proteins to execute unusual tricks.
Pentapeptide scanning mutagenesis is a facile transposon-based procedure for the random insertion of a variable five amino acid cassette into a target protein. The analysis of a library of proteins harbouring pentapeptide insertions can provide invaluable information on the essential and inessential regions of a target protein, as well as revealing surprising aspects of target protein function and activity.
Hayes
F
F
Dept of Biomolecular Sciences, University of Manchester Institute of Science and Technology (UMIST), PO Box 88, M60 1QD, Manchester, UK. finbarr.hayes@bi.umist.ac.uk
Hallet
B
B
D000595:Amino Acid Sequence; D000818:Animals; D004251:DNA Transposable Elements; D006801:Humans; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000379:methods*; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics*; D019151:Peptide Library; D011506:Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D004251:DNA Transposable Elements; D010446:Peptide Fragments; D019151:Peptide Library; D011506:Proteins