title: Recurrent (nonfamilial) hemangioblastomas involving spinal nerve roots: case report.
abstract: OBJECTIVE
  Spinal nerve root hemangioblastomas are rare and are reported mainly in patients with von Hippel-Lindau (VHL) syndrome. The pathogenesis of so-called nonfamilial lesions is virtually unknown. We discuss, mainly from a molecular perspective, a unique patient with sporadic, recurrent hemangioblastomas restricted to spinal nerve roots.
  
  
  METHODS
  A 53-year-old man who had had a surgically corrected lumbosacral meningomyelocele presented on at least three occasions during a 17-year period with multifocal capillary hemangioblastomas involving spinal nerve roots. On each occasion, tumors appeared on a different nerve root, with the majority located in the midcervical segments. The patient had no clinical features or family history of VHL syndrome.
  
  
  METHODS
  To obtain a clearer understanding of the pathogenesis of this unusual case and its relationship to VHL syndrome, molecular analysis of the VHL gene was performed by use of complete sequence analysis and loss of heterozygosity studies on deoxyribonucleic acid derived from the patient's blood leukocytes and three separately resected hemangioblastomas.
  
  
  CONCLUSIONS
  Germ-line molecular analysis performed on all three exons in the VHL gene coding region did not indicate that any mutations were present. Loss of heterozygosity analysis of deoxyribonucleic acid from the three hemangioblastoma resections showed normal heterozygosity in the 3p25-26 region. Complete VHL gene sequence analysis did not demonstrate a somatic mutation in the coding region of the VHL gene in any of the three tumors, thereby supporting the loss of heterozygosity data that a molecular event directly involving the VHL gene may not be the causative factor in their tumorigenesis.
authors: Department of Pathology, University of Texas Southwestern Medical Center, Dallas 75235-9073, USA. rragha@mednet.swmed.edu
authors: R
authors: R
authors: Raghavan
authors: J
authors: J
authors: Krumerman
authors: E J
authors: EJ
authors: Rushing
authors: C L
authors: CL
authors: White
authors: D P
authors: DP
authors: Chason
authors: M L
authors: ML
authors: Watson
authors: C
authors: C
authors: Coimbra
mesh_terms: D001483:Base Sequence / Q000235:genetics; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000235:genetics; D018325:Hemangioblastoma / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D006801:Humans; D019656:Loss of Heterozygosity; D008279:Magnetic Resonance Imaging; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local; D010524:Peripheral Nervous System Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D013126:Spinal Nerve Roots
chemical_list: D004273:DNA, Neoplasm