On the galactosyl distribution of commercial galactomannans.
A simple method was developed that enabled the enzymatic determination of the galactose distribution in galactomannans. endo-Mannanase of Aspergillus niger was used to degrade the galactomannan polymers and the degradation products were determined with high-performance anion-exchange chromatography. A whole range of commercial high-to-low substituted galactomannans was analyzed in this way. It was found that differences in the anion-exchange chromatograms reflected dissimilarities in the distribution of galactose and could be used directly to discern these dissimilarities. The differences among the various elution profiles were used to construct a similarity distance tree. In addition to this approach, the absolute amount of non-substituted mannose released by the enzyme was found to be a good discriminating factor. In this way, galactomannans with regular, blockwise, and randomly distributed galactose could be discerned. All guars and the highly substituted gum of Prosopis juliflora were found to have a blockwise distribution of galactose. For different batches of tara gum both random and blockwise distributions were found. Among batches of locust bean gum the greatest variation was observed: both random, blockwise, and ordered galactose distributions were present. Cassia gum was found to have a highly regular distribution of galactose.
Daas
P J
PJ
Wageningen Centre for Food Sciences, The Netherlands.
Schols
H A
HA
de Jongh
H H
HH
D002852:Chromatography, Ion Exchange; D005685:Galactans / Q000737:chemistry; D005690:Galactose / Q000032:analysis / Q000031:analogs & derivatives; D008351:Mannans / Q000737:chemistry* / Q000302:isolation & purification; D008358:Mannose / Q000032:analysis; D008361:Mannosidases; D053149:Plant Gums; D044902:beta-Mannosidase
D005685:Galactans; D008351:Mannans; D053149:Plant Gums; C012990:galactomannan; C007894:guar gum; D008361:Mannosidases; D044902:beta-Mannosidase; D008358:Mannose; D005690:Galactose