title: Regulation of Wee1 kinase in response to protein synthesis inhibition.
abstract: To investigate the mechanism coupling growth (protein synthesis) with cell division, we examined the relationship between the tyrosine kinase Wee1 that inhibits Cdc2-Cdc13 mitosis-inducing kinase by phosphorylating it, and protein synthesis inhibition in fission yeast. The wee1-50 mutant showed supersensitivity to protein synthesis inhibitor, cycloheximide. Wee1 was essential for the G(2) delay upon a partial inhibition of protein synthesis. Indeed, the protein synthesis inhibition caused an increase in the Wee1 protein by the Sty1/Spc1 MAPK-dependent transcriptional and the Sty1/Spc1 MAPK-independent post-transcriptional regulations. Further, the results indicated that the post-transcriptional regulation is important for the G(2) delay.
authors: Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Japan.
authors: M
authors: M
authors: Suda
authors: S
authors: S
authors: Yamada
authors: T
authors: T
authors: Toda
authors: T
authors: T
authors: Miyakawa
authors: D
authors: D
authors: Hirata
mesh_terms: D016203:CDC2 Protein Kinase / Q000235:genetics / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects / Q000235:genetics; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects / Q000235:genetics; D019926:Cyclin B / Q000235:genetics / Q000378:metabolism; D003513:Cycloheximide / Q000494:pharmacology; D016195:G2 Phase / Q000187:drug effects / Q000235:genetics; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D008938:Mitosis / Q000235:genetics; D009687:Nuclear Proteins; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000378:metabolism; D012568:Schizosaccharomyces / Q000187:drug effects* / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics
chemical_list: D018797:Cell Cycle Proteins; D019926:Cyclin B; D009687:Nuclear Proteins; D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; D029702:Schizosaccharomyces pombe Proteins; D003513:Cycloheximide; C068689:wee1 protein, S pombe; D011505:Protein-Tyrosine Kinases; D016203:CDC2 Protein Kinase; D020928:Mitogen-Activated Protein Kinases; C095409:sty1 protein, S pombe