CINC blockade prevents neutrophil Ca(2+) signaling upregulation and gut bacterial translocation in thermal injury.
In this study, we have evaluated the role of cytokine-induced neutrophil chemoattractant (CINC), in the upregulation of neutrophil Ca(2+) signaling in neutrophils from thermally injured rats treated with anti-CINC antibody. Additionally, we have determined the effect of the treatment with CINC antibody on the accumulation of activated neutrophils in the intestinal wall, and the effect of such accumulation on gut bacterial translocation. Measurements of myeloperoxidase (MPO) activity and immunohistochemical localization of neutrophils determined neutrophil sequestration in the rat intestine. Agar culture analyses and a specific Escherichia coli beta-galactosidase gene polymerase chain reaction was carried out to detect gut indigenous bacterial invasion into intestinal wall and extraintestinal mesenteric lymph nodes (MLN). The results showed that pretreatment of rats with anti-CINC antibody attenuated the thermal injury-induced enhancement in [Ca(2+)](i) responses in neutrophils both in the basal and Formyl-Met-Leu-Phe stimulated conditions. Moreover, treatment with the CINC antibody decreased neutrophil infiltration into the gut and attenuated thermal injury-caused translocation of bacteria into the MLN.
Fazal
N
N
Department of Surgery, Loyola University Chicago Medical Center, Maywood, Illinois 60153, USA.
Shamim
M
M
Zagorski
J
J
Choudhry
M A
MA
Ravindranath
T
T
Sayeed
M M
MM
D000818:Animals; D000906:Antibodies / Q000627:therapeutic use*; D002056:Burns / Q000276:immunology* / Q000628:therapy; D002118:Calcium / Q000032:analysis; D020013:Calcium Signaling / Q000276:immunology; D019743:Chemokines, CXC / Q000037:antagonists & inhibitors / Q000276:immunology; D002630:Chemotactic Factors / Q000037:antagonists & inhibitors* / Q000276:immunology; D004269:DNA, Bacterial / Q000032:analysis; D004926:Escherichia coli / Q000187:drug effects* / Q000302:isolation & purification; D006133:Growth Substances / Q000276:immunology; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins; D007413:Intestinal Mucosa / Q000276:immunology / Q000382:microbiology; D008198:Lymph Nodes / Q000276:immunology / Q000382:microbiology; D008297:Male; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology / Q000502:physiology; D009195:Peroxidase / Q000032:analysis; D010581:Peyer's Patches / Q000276:immunology / Q000382:microbiology; D051381:Rats; D017207:Rats, Sprague-Dawley
D000906:Antibodies; D019743:Chemokines, CXC; D002630:Chemotactic Factors; D004269:DNA, Bacterial; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009195:Peroxidase; D002118:Calcium