Kidney produces a novel acylated peptide, ghrelin.
Ghrelin is a novel growth hormone-releasing peptide with a unique acylated structure. Here we reveal that prepro-ghrelin gene is expressed in the mouse kidney and glomerulus. We also show by reverse-phase high performance liquid chromatography coupled with radioimmunoassay that the mouse kidney does produce ghrelin. The ghrelin immunoreactivity in the mouse kidney is 6.79+/-0.48 fmol/mg (n=5), which is much more abundant than that in the mouse plasma of 0.339+/-0.029 fmol/microl (n=6). Furthermore, prepro-ghrelin gene is expressed in cultured rat mesangial cells, fibroblast-like NRK-49F cells and mouse podocytes, but not in rat epithelial cell-like NRK-52E cells. Ghrelin receptor gene is also expressed in the rat kidney. These findings demonstrate that the kidney, glomerulus and renal cells express prepro-ghrelin gene and ghrelin is produced locally in the kidney, and suggest the endocrine and/or paracrine roles of ghrelin in the kidney.
Mori
K
K
Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Kyoto, Japan.
Yoshimoto
A
A
Takaya
K
K
Hosoda
K
K
Ariyasu
H
H
Yahata
K
K
Mukoyama
M
M
Sugawara
A
A
Hosoda
H
H
Kojima
M
M
Kangawa
K
K
Nakao
K
K
D000215:Acylation; D000818:Animals; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D015870:Gene Expression; D054439:Ghrelin; D005920:Glomerular Mesangium / Q000166:cytology / Q000378:metabolism; D007668:Kidney / Q000166:cytology / Q000378:metabolism*; D007678:Kidney Glomerulus / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D036361:Peptide Hormones; D010455:Peptides / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D011863:Radioimmunoassay; D051381:Rats; D011921:Rats, Inbred WKY; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000235:genetics; D043562:Receptors, G-Protein-Coupled; D054440:Receptors, Ghrelin; D020133:Reverse Transcriptase Polymerase Chain Reaction
D054439:Ghrelin; D036361:Peptide Hormones; D010455:Peptides; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D054440:Receptors, Ghrelin