S100A1 and S100B interactions with annexins.
Members of the annexin protein family interact with members of the S100 protein family thereby forming heterotetramers in which an S100 homodimer crossbridges two copies of the pertinent annexin. Previous work has shown that S100A1 and S100B bind annexin VI in a Ca(2+)-dependent manner and that annexin VI, but not annexin V, blocks the inhibitory effect of S100A1 and S100B on intermediate filament assembly. We show here that both halves of annexin VI (i.e., the N-terminal half or annexin VI-a and the C-terminal half or annexin VI-b) bind individual S100s on unique sites and that annexin VI-b, but not annexin VI-a, blocks the ability of S100A1 and S100B to inhibit intermediate filament assembly. We also show that the C-terminal extension of S100A1 (and, by analogy, S100B), that was previously demonstrated to be critical for S100A1 and S100B binding to several target proteins including intermediate filament subunits, is not part of the S100 surface implicated in the recognition of annexin VI, annexin VI-a, or annexin VI-b. Evaluation of functional properties with a liposome stability and a calcium influx assay reveals the ability of both S100 proteins to permeabilize the membrane bilayer in a similar fashion like annexins. When tested in combinations with different annexin proteins both S100 proteins mostly lead to a decrease in the calcium influx activity although not all annexin/S100 combinations behave in the same manner. Latter observation supports the hypothesis that the S100-annexin interactions differ mechanistically depending on the particular protein partners.
Garbuglia
M
M
Department of Experimental Medicine and Biochemical Sciences, Section of Anatomy, University of Perugia, Italy.
Verzini
M
M
Hofmann
A
A
Huber
R
R
Donato
R
R
D000818:Animals; D017317:Annexin A6 / Q000737:chemistry; D017302:Annexins / Q000737:chemistry*; D015153:Blotting, Western; D002118:Calcium / Q000737:chemistry*; D002135:Calcium-Binding Proteins / Q000737:chemistry*; D011232:Chemical Precipitation; D003432:Cross-Linking Reagents; D019281:Dimerization; D004591:Electrophoresis, Polyacrylamide Gel; D005452:Fluoresceins; D005904:Glial Fibrillary Acidic Protein / Q000737:chemistry; D007381:Intermediate Filament Proteins / Q000737:chemistry; D008081:Liposomes; D009414:Nerve Growth Factors / Q000737:chemistry*; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; D013050:Spectrometry, Fluorescence; D013388:Succinimides
D017317:Annexin A6; D017302:Annexins; D002135:Calcium-Binding Proteins; D003432:Cross-Linking Reagents; D005452:Fluoresceins; D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; D008081:Liposomes; D009414:Nerve Growth Factors; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; C110294:S100A1 protein; D013388:Succinimides; C024098:6-carboxyfluorescein; D002118:Calcium; C019358:disuccinimidyl suberate