Identification of 6-furfuryladenine (kinetin) in human urine.
In contrast to the current view of kinetin (K, N(6)-furfuryladenine) as an unnatural and synthetic cytokinin, recently it has been identified in plant DNA and plant extract. Here we describe identification of K in human urine using chromatography/mass-spectrometry analysis for the first time. The amount of kinetin in urine taken from unhealthy patients lung carcinoma was established to be 0.5 ng in 20 ml and a 100-fold reduced amount in healthy subjects. Since this rare base is a potential source of structural constrains it has to be removed from DNA by enzymatic DNA-repair reactions. It seems that the presence of kinetin in human is linked to oxidative damage processes.
Barciszewski
J
J
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12, 61704 Poznan, Poland. jbarcisz@ibch.poznan.pl
Mielcarek
M
M
Stobiecki
M
M
Siboska
G
G
Clark
B F
BF
D000225:Adenine / Q000031:analogs & derivatives* / Q000652:urine*; D016022:Case-Control Studies; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007701:Kinetin; D008175:Lung Neoplasms / Q000652:urine
D000225:Adenine; D007701:Kinetin