The class IV semaphorin CD100 plays nonredundant roles in the immune system: defective B and T cell activation in CD100-deficient mice.
The class IV semaphorin CD100/Sema4D differentially utilizes two distinct receptors: plexin-B1 in nonlymphoid tissues, such as brain and kidney, and CD72 in lymphoid tissues. We have generated CD100-deficient mice and demonstrated that they have functional defects in their immune system, without apparent abnormalities in other tissues. The number of CD5(+) B-1 cells was considerably decreased in the mutant mice, whereas conventional B cells and T cells appeared to develop normally. In vitro proliferative responses and immunoglobulin production were reduced in CD100-deficient B cells. The humoral immune response against a T cell-dependent antigen and in vivo priming of T cells were also defective in the mutant mice. These results demonstrate nonredundant and essential roles of CD100-CD72 interactions in the immune system.
Shi
W
W
Department of Molecular Immunology, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamada-oka, Suita Osaka 565-0871, Japan.
Kumanogoh
A
A
Watanabe
C
C
Uchida
J
J
Wang
X
X
Yasui
T
T
Yukawa
K
K
Ikawa
M
M
Okabe
M
M
Parnes
J R
JR
Yoshida
K
K
Kikutani
H
H
D000818:Animals; D015703:Antigens, CD; D001402:B-Lymphocytes / Q000276:immunology*; D005786:Gene Expression Regulation / Q000276:immunology; D007109:Immunity / Q000235:genetics; D008213:Lymphocyte Activation / Q000235:genetics* / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000276:immunology*; D051379:Mice; D018345:Mice, Knockout; D039961:Semaphorins; D013601:T-Lymphocytes / Q000276:immunology*
D015703:Antigens, CD; C093224:CD100 antigen; D008562:Membrane Glycoproteins; D039961:Semaphorins