title: Structural rearrangements and insertions of dispersed elements in pericentromeric alpha satellites occur preferably at kinkable DNA sites.
abstract: Centromeric region of human chromosome 21 comprises two long alphoid DNA arrays: the well homogenized and CENP-B box-rich alpha21-I and the alpha21-II, containing a set of less homogenized and CENP-B box-poor subfamilies located closer to the short arm of the chromosome. Continuous alphoid fragment of 100 monomers bordering the non-satellite sequences in human chromosome 21 was mapped to the pericentromeric short arm region by fluorescence in situ hybridization (alpha21-II locus). The alphoid sequence contained several rearrangements including five large deletions within monomers and insertions of three truncated L1 elements. No binding sites for centromeric protein CENP-B were found. We analyzed sequences with alphoid/non-alphoid junctions selectively screened from current databases and revealed various rearrangements disrupting the regular tandem alphoid structure, namely, deletions, duplications, inversions, expansions of short oligonucleotide motifs and insertions of different dispersed elements. The detailed analysis of more than 1100 alphoid monomers from junction regions showed that the vast majority of structural alterations and joinings with non-alphoid DNAs occur in alpha satellite families lacking CENP-B boxes. Most analyzed events were found in sequences located toward the edges of the centromeric alphoid arrays. Different dispersed elements were inserted into alphoid DNA at kinkable dinucleotides (TG, CA or TA) situated between pyrimidine/purine tracks. DNA rearrangements resulting from different processes such as recombination and replication occur at kinkable DNA sites alike insertions but irrespectively of the occurrence of pyrimidine/purine tracks. It seems that kinkable dinucleotides TG, CA and TA are part of recognition signals for many proteins involved in recombination, replication, and insertional events. Alphoid DNA is a good model for studying these processes.
authors: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov Str., Moscow, 117984, Russia. mashkova@genome.eimb.relarn.ru
authors: T D
authors: TD
authors: Mashkova
authors: N Y
authors: NY
authors: Oparina
authors: M H
authors: MH
authors: Lacroix
authors: L I
authors: LI
authors: Fedorova
authors: I
authors: I
authors: G Tumeneva
authors: O L
authors: OL
authors: Zinovieva
authors: L L
authors: LL
authors: Kisselev
mesh_terms: D020087:Alu Elements / Q000235:genetics; D001324:Autoantigens; D001483:Base Sequence; D001665:Binding Sites; D002503:Centromere / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D051936:Centromere Protein B; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D002872:Chromosome Deletion; D007446:Chromosome Inversion; D002891:Chromosomes, Human, Pair 21 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D019295:Computational Biology; D003434:Crossing Over, Genetic / Q000235:genetics; D004261:DNA Replication / Q000235:genetics; D004276:DNA, Satellite / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins; D019992:Databases as Topic; D018900:Dinucleotide Repeats / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes; D016254:Mutagenesis, Insertional / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic / Q000235:genetics*
chemical_list: D001324:Autoantigens; C497926:CENPB protein, human; D051936:Centromere Protein B; D002868:Chromosomal Proteins, Non-Histone; D004276:DNA, Satellite; D004268:DNA-Binding Proteins