Recent advances in the protocols of transgenic mouse mutation assays.
Transgenic mutation assays were developed to detect gene mutations in multiple organs of mice or rats. The assays permit (1) quantitative measurements of mutation frequencies in all tissues/organs including germ cells and (2) molecular analysis of induced and spontaneous mutations by DNA sequencing analysis. The protocols of recently developed selections in the lambda phage-based transgenic mutation assays, i.e. cII, Spi(-) and 6-thioguanine selections, are described, and a data set of transgenic mutation assays, including those using Big Blue and Muta Mouse, is presented.
Nohmi
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Division of Genetics and Mutagenesis, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, 158-8501, Tokyo, Japan. nohmi@nihs.go.jp
Suzuki
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Masumura
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D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D016296:Mutagenesis; D009152:Mutagenicity Tests / Q000379:methods*