An investigation into the pathogenic properties of Escherichia coli strains BLR, BL21, DH5alpha and EQ1.
OBJECTIVE
To examine Escherichia coli strains EQ1, DH5alpha, BLR and BL21 for known pathogenic mechanisms.


RESULTS
Using specific DNA probes, the strains were shown not to carry the genes encoding invasion, various adhesion phenotypes or expression of a range of enterotoxins. The strains were unable to express long-chain lipopolysaccharide and were susceptible to the effects of serum complement. Using a BALB/c mouse model, the strains were shown to be unable to survive in selected tissues or to persist in the mouse gut. Using a chick model, strains EQ1, BLR and BL21 invaded livers but not spleens; only strain EQ1 persisted in the chick gut. In Merino sheep, only strain EQ1 was detected 6 d post-infection.


CONCLUSIONS
Escherichia coli strains EQ1, DH5alpha, BLR and BL21 did not carry the well-recognized pathogenic mechanisms required by strains of E. coli causing the majority of enteric infections.


CONCLUSIONS
Escherichia coli strains EQ1, DH5alpha, BLR and BL21 were considered to be non-pathogenic and unlikely to survive in host tissues and cause disease.
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Laboratory of Enteric Pathogens, Division of Gastrointestinal Infections, Central Public Health Laboratory, London, UK. h.chart@nhs.co.uk
Smith
H R
HR
La Ragione
R M
RM
Woodward
M J
MJ
D000818:Animals; D001422:Bacterial Adhesion / Q000235:genetics; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis; D001427:Bacterial Toxins / Q000235:genetics; D001769:Blood / Q000382:microbiology; D002645:Chickens; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity* / Q000648:ultrastructure; D004927:Escherichia coli Infections / Q000382:microbiology*; D005798:Genes, Bacterial; D006384:Hemagglutination; D006863:Hydrogen-Ion Concentration; D008070:Lipopolysaccharides / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D012756:Sheep; D017262:Siderophores / Q000378:metabolism; D014774:Virulence / Q000235:genetics
D001425:Bacterial Outer Membrane Proteins; D001427:Bacterial Toxins; D008070:Lipopolysaccharides; D017262:Siderophores