Identification of the phospholipid-binding site of human beta(2)-glycoprotein I domain V by heteronuclear magnetic resonance.
To understand the mechanism of the interaction between human beta(2)-glycoprotein I (beta(2)-GPI) and negatively charged phospholipids, we determined the three-dimensional solution structure of the fifth domain of beta(2)-GPI by heteronuclear multidimensional NMR. The results showed that the molecule is composed of well-defined four anti-parallel beta-strands and two short alpha-helices, as well as a long highly flexible loop. Backbone dynamic analysis demonstrated significant mobility of the flexible loop on a subnanosecond time scale. Structural modeling of the nicked fifth domain, in which the Lys317-Thr318 peptide bond was specifically cleaved, revealed the importance of this long C-terminal loop for the interaction between beta(2)-GPI and negatively charged phospholipids. A titration experiment with the anionic surfactant SDS showed that this highly mobile loop, as well as the short beta-hairpin between betaC and betaD strands, which is rich in positively charged residues, specifically interact with the surfactant. The mobile loop, together with the surrounding positively charged residues, probably construct the binding site for negatively charged phospholipids such as cardiolipin.
Hoshino
M
M
Institute for Protein Research, Osaka University, Yamadaoka 3-2, Suita, 565-0871, Japan.
Hagihara
Y
Y
Nishii
I
I
Yamazaki
T
T
Kato
H
H
Goto
Y
Y
D000595:Amino Acid Sequence; D001665:Binding Sites; D006023:Glycoproteins / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D006860:Hydrogen Bonding; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D010743:Phospholipids / Q000378:metabolism*; D018583:Pliability; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D012967:Sodium Dodecyl Sulfate / Q000378:metabolism; D055672:Static Electricity; D013329:Structure-Activity Relationship; D017186:Titrimetry; D053482:beta 2-Glycoprotein I
D006023:Glycoproteins; D010743:Phospholipids; D053482:beta 2-Glycoprotein I; D012967:Sodium Dodecyl Sulfate