title: Capillary electrophoresis and off-line capillary electrophoresis-electrospray ionization quadrupole time-of-flight tandem mass spectrometry of carbohydrates.
abstract: The use of off-line high-performance capillary electrophoresis in connection with nanospray electrospray ionization quadrupole time-of-flight tandem mass spectrometry for identification of complex carbohydrates of biological origin is presented. The method was applied to the identification of O-glycosylated amino acids and -glycopeptides from the urine of patients suffering from a hereditary disease - N-acetylhexosaminidase deficiency. Structural elements typical for O-glycosylation of proteins, like expression of core 1 and 2 type O-glycans with different numbers of N-acetyllactosaminyl repeats and different degrees of sialylation, can be directly detected.
authors: Institute for Medical Physics and Biophysics, University of Münster, Germany.
authors: A
authors: A
authors: Zamfir
authors: S
authors: S
authors: König
authors: J
authors: J
authors: Althoff
authors: J
authors: J
authors: Peter-Katalinc
mesh_terms: D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D002241:Carbohydrates