Determination of D1 and D2 dopamine receptor expression by Ntera-2 cells.
There is evidence that D1 and D2 dopamine receptors are co-expressed on some neurons. As a potential model of co-expression we examined Ntera-2 cells using RT-PCR, and showed that they express D2 but not D1 receptors. D2 dopamine receptor expression was confirmed by quinpirole inhibition of forskolin-stimulated cAMP formation. Absence of D1 dopamine receptors was confirmed by the inability of dopamine or SKF 81297 to increase cAMP.
Sakolsky
D J
DJ
Department of Pharmacology, Temple University School of Medicine, 3400 North Broad Street, Philadelphia, PA 19140, USA.
Ashby
B
B
D001552:Benzazepines / Q000494:pharmacology; D005576:Colforsin / Q000037:antagonists & inhibitors / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D004298:Dopamine / Q000494:pharmacology; D018491:Dopamine Agonists / Q000494:pharmacology; D006801:Humans; D019257:Quinpirole / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D017447:Receptors, Dopamine D1 / Q000235:genetics* / Q000378:metabolism; D017448:Receptors, Dopamine D2 / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018243:Teratocarcinoma / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured
D001552:Benzazepines; D018491:Dopamine Agonists; D012333:RNA, Messenger; D017447:Receptors, Dopamine D1; D017448:Receptors, Dopamine D2; D005576:Colforsin; D019257:Quinpirole; C067113:SK&F 81297; D000242:Cyclic AMP; D004298:Dopamine