Consensus predictions of membrane protein topology.
We have explored the possibility that consensus predictions of membrane protein topology might provide a means to estimate the reliability of a predicted topology. Using five current topology prediction methods and a test set of 60 Escherichia coli inner membrane proteins with experimentally determined topologies, we find that prediction performance varies strongly with the number of methods that agree, and that the topology of nearly half of all E. coli inner membrane proteins can be predicted with high reliability (>90% correct predictions) by a simple majority-vote approach.
Nilsson
J
J
Stockholm Bioinformatics Center, Department of Biochemistry, Stockholm University, Sweden.
Persson
B
B
von Heijne
G
G
D001426:Bacterial Proteins / Q000737:chemistry*; D019295:Computational Biology; D003198:Computer Simulation; D004926:Escherichia coli; D008565:Membrane Proteins / Q000737:chemistry*; D008956:Models, Chemical; D011237:Predictive Value of Tests; D017510:Protein Folding; D017434:Protein Structure, Tertiary / Q000502:physiology; D015203:Reproducibility of Results
D001426:Bacterial Proteins; D008565:Membrane Proteins