Ovarian tumor cells express a transmembrane serine protease: a potential candidate for early diagnosis and therapeutic intervention.
Proteases have been implicated in the extracellular modulation required for tumor growth and invasion. In an effort to categorize those proteases contributing to ovarian carcinoma progression, we have utilized redundant primers to conserved amino acid (AA) domains surrounding the catalytic triad of His, Asp and Ser to amplify serine proteases that are differentially expressed in carcinomas. Using this method, we have identified and cloned a serine protease named TADG-15 (tumor-associated differentially expressed gene 15) that is overexpressed in ovarian tumors. TADG-15 is a transmembrane multidomain serine protease which includes ligand binding domains and a serine protease in the extracellular space.
Tanimoto
H
H
Department of Obstetrics and Gynecology, Asada General Hospital, Kagawa, Japan.
Underwood
L J
LJ
Wang
Y
Y
Shigemasa
K
K
Parmley
T H
TH
O'Brien
T J
TJ
D000595:Amino Acid Sequence; D000818:Animals; D001224:Aspartic Acid / Q000737:chemistry; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D020134:Catalytic Domain; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D003001:Cloning, Molecular; D017931:DNA Primers / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D005260:Female; D006639:Histidine / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry; D008024:Ligands; D051379:Mice; D008954:Models, Biological; D008969:Molecular Sequence Data; D010051:Ovarian Neoplasms / Q000175:diagnosis* / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology; D010053:Ovary / Q000378:metabolism / Q000473:pathology; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012694:Serine / Q000737:chemistry; D012697:Serine Endopeptidases / Q000096:biosynthesis*; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D018076:DNA, Complementary; D008024:Ligands; D012333:RNA, Messenger; D001224:Aspartic Acid; D012694:Serine; D006639:Histidine; D012697:Serine Endopeptidases