Orchestrated transcription of key pathways in Arabidopsis by the circadian clock.
Like most organisms, plants have endogenous biological clocks that coordinate internal events with the external environment. We used high-density oligonucleotide microarrays to examine gene expression in Arabidopsis and found that 6% of the more than 8000 genes on the array exhibited circadian changes in steady-state messenger RNA levels. Clusters of circadian-regulated genes were found in pathways involved in plant responses to light and other key metabolic pathways. Computational analysis of cycling genes allowed the identification of a highly conserved promoter motif that we found to be required for circadian control of gene expression. Our study presents a comprehensive view of the temporal compartmentalization of physiological pathways by the circadian clock in a eukaryote.
Harmer
S L
SL
Department of Cell Biology, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Hogenesch
J B
JB
Straume
M
M
Chang
H S
HS
Han
B
B
Zhu
T
T
Wang
X
X
Kreps
J A
JA
Kay
S A
SA
D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D001683:Biological Clocks / Q000235:genetics*; D050260:Carbohydrate Metabolism; D002940:Circadian Rhythm; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008027:Light; D009584:Nitrogen / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010788:Photosynthesis / Q000235:genetics; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D013455:Sulfur / Q000378:metabolism; D014158:Transcription, Genetic
D045322:Photosynthetic Reaction Center Complex Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D013455:Sulfur; D009584:Nitrogen