Epigenes: design and construction of new hereditary units.
A plasmid digene construction designed before [Tchuraev, R.N. (1982) J. Gen. Biol. 43, 79-87] has been realised, including feedback by repressing proteins with given trigger regime of gene functioning. Experimental tests of the expected epigene properties of the obtained pECPI recombinant plasmid involving lacI and cI(857) regulatory genes have shown a phenomenon of steady inheritance of two alternative epigenotypes lacI(1)cI(0) and lacI(0)cI(1), as well as an external toggle switch through metabolitic and temperature signals from one inherited functional state of the cyclic digene system into another. Thus, we have constructed a hereditary unit of a specific kind, namely, a two-component stationary epigene with preset properties.
Tchuraev
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Institute of Biology, Ufa Research Centre, Russian Academy of Sciences, 69 Prospect Oktyabrya, 450054, Ufa, Russia. tchuraev@anrb.ru
Stupak
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Tropynina
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Stupak
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D001426:Bacterial Proteins / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D005111:Extrachromosomal Inheritance / Q000235:genetics*; D005246:Feedback; D015964:Gene Expression Regulation, Bacterial; D005809:Genes, Regulator / Q000235:genetics; D017930:Genes, Reporter; D005818:Genetic Engineering / Q000379:methods*; D018392:Genomic Imprinting / Q000235:genetics*; D007763:Lac Operon; D056968:Lac Repressors; D010957:Plasmids / Q000235:genetics*; D011995:Recombination, Genetic; D012097:Repressor Proteins / Q000235:genetics
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D056968:Lac Repressors; C539064:LacI protein, E coli; D012097:Repressor Proteins