Capillary electrophoretic determination of apoptosis of HeLa cells induced by trichosanthin.
Apoptosis is a distinct mode of cell death that is responsible for deletion of cells in normal tissues; it also occurs in specific pathologic contexts. The observation of apoptosis is very important in the research of cancer and cancer therapy. The traditional observation method of apoptosis was agarose gel electrophoresis, which is depending on the determination of ladder-liking DNA fragments extracted from apoptotic cells. It is time-consuming and low-sensitive. Recently, the sieving capillary electrophoresis has been used to detect apoptosis too. However, the problem of DNA fragments contamination is still existing. Here, we have developed a capillary electrophoresis method that could detect apoptosis of whole cell directly and do not need to extract DNA fragments from cells. Apoptosis of adherent cell HeLa cell of carcinoma induced by cyclophosphamide was used as the model to establish the method. The effluence of medicine concentration on apoptosis of cells was studied in detail. It was also found that the method could detect the change of cells in the early period of apoptosis. The induction of apoptosis of HeLa cell by trichosanthin was determined with the method, and the result of flow cytometry was also proved that trichosanthin could result in apoptosis of HeLa cells.
Ru
Q H
QH
School of Life Science and Engineering, Tsinghua University, Beijing, PR China.
Luo
G A
GA
Liao
J J
JJ
Liu
Y
Y
D017209:Apoptosis / Q000187:drug effects*; D003520:Cyclophosphamide / Q000494:pharmacology; D019075:Electrophoresis, Capillary / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D015978:Trichosanthin / Q000494:pharmacology*
D015978:Trichosanthin; D003520:Cyclophosphamide