Full structure of the O-specific polysaccharide of Proteus mirabilis O24 containing 3,4-O-[(S)-1-carboxyethylidene]-D-galactose.
The structure of a neutral polysaccharide isolated by degradation with dilute acetic acid of the lipopolysaccharide (LPS) of P. mirabilis O24 has been determined recently [E. Literacka et al., FEBS Lett., 456 (1999) 227-231]. Further studies of this LPS using alkaline degradation and hydrolysis at pH 4.5 showed that the polysaccharide chain includes an acetal-linked pyruvic acid residue, which is removed completely during delipidation with acetic acid. A revision using 1H and 13C NMR spectroscopy and methylation analysis resulted in determination of the following full structure of the repeating unit of the O-specific polysaccharide: carbohydrate sequence [see text] where D-Gal3,4(S-Pyr) is 3,4-O-[(S)-1-carboxyethylidene]-D-galactose.
Shashkov
A S
AS
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow.
Senchenkova
S N
SN
Vinogradov
E V
EV
Zatonsky
G V
GV
Knirel
Y A
YA
Literacka
E
E
Kaca
W
W
D019342:Acetic Acid / Q000494:pharmacology; D002240:Carbohydrate Sequence; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry* / Q000302:isolation & purification; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D011513:Proteus mirabilis / Q000737:chemistry* / Q000145:classification; D019289:Pyruvic Acid / Q000032:analysis; D012703:Serotyping
D019081:O Antigens; D011135:Polysaccharides, Bacterial; D019289:Pyruvic Acid; D019342:Acetic Acid