Interleukin-12 induces gene expression in interleukin-2 stimulated human T lymphocytes.
IL-12 is a critical immunoregulatory cytokine that promotes cell-mediated immune responses by inducing the differentiation of Th1 cells. To better clarify the molecular basis of IL-12 action, we compared the gene expression in human T lymphocytes activated by IL-2 and IL-12. mRNAs from T lymphocytes activated by either IL-2 alone or IL-2 plus IL-12 were transcribed into cDNAs. A differential mRNA display was conducted. As a result, differential display of five cDNA fragments was obtained. Sequence analysis suggests that they had high homology with recorded genes as found by a computer search against GenBank. Two full genes of the five fragments were cloned, which activation-induced C-type lectin and glucose transporter-like protein. Interestingly, these proteins were expressed in the T cells stimulated by IL-2 and IL-12, but not in the T cells stimulated by IL-2 alone. These results suggest that C-type lectin and glucose transporter-like protein may play an important role in the T lymphocyte activation induced by IL-12.
Li
B
B
Department of Immunology, Harbin Medical University, Harbin 150086, PR China.
Cao
D
D
Xu
H
H
Chang
J
J
Zhou
G
G
Tian
J
J
Li
D
D
Theze
J
J
Wu
C
C
D001483:Base Sequence; D002478:Cells, Cultured; D020869:Gene Expression Profiling; D051274:Glucose Transporter Type 3; D006801:Humans; D018664:Interleukin-12 / Q000494:pharmacology*; D007376:Interleukin-2 / Q000494:pharmacology*; D037102:Lectins / Q000096:biosynthesis* / Q000235:genetics; D037181:Lectins, C-Type; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins / Q000096:biosynthesis* / Q000235:genetics; D009419:Nerve Tissue Proteins; D012689:Sequence Homology, Nucleic Acid; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D015533:Transcriptional Activation / Q000187:drug effects
D051274:Glucose Transporter Type 3; D007376:Interleukin-2; D037102:Lectins; D037181:Lectins, C-Type; D009004:Monosaccharide Transport Proteins; D009419:Nerve Tissue Proteins; C495687:SLC2A3 protein, human; D018664:Interleukin-12