title: Polysomes of eukaryotic cells observed by electron microscopy.
abstract: Shapes of polysomes of eukaryotic cells were determined by surface spreading of cells. We examined unicellular protozoan Tetrahymena cells, rabbit reticulocytes, and cultured MDCK, BHK and HeLa cells. Polysomes had ring-, 8- and caterpillar-shaped forms, indicating that eukaryotic cells contain fundamentally circular polysomes. Isolated and partially purified polysomes from Tetrahymena cells had features of polysomes similar to those of spread cells. These findings indicate that circular polysomes are not an effect of the spreading, but are actual features of the cells.
authors: Department of Ultrastructural Research, Tokyo Metropolytan Institute of Medical Science, Japan. yazaki@rinshoken.or.jp
authors: K
authors: K
authors: Yazaki
authors: T
authors: T
authors: Yoshida
authors: M
authors: M
authors: Wakiyama
authors: K
authors: K
authors: Miura
mesh_terms: D000818:Animals; D002460:Cell Line; D005057:Eukaryotic Cells / Q000648:ultrastructure*; D006367:HeLa Cells; D006801:Humans; D008854:Microscopy, Electron / Q000379:methods*; D011132:Polyribosomes / Q000648:ultrastructure*; D011817:Rabbits; D012156:Reticulocytes / Q000648:ultrastructure; D013768:Tetrahymena / Q000648:ultrastructure