Parallel monitoring of mitotic recombination, clastogenicity and teratogenic effects in eye tissue of Drosophila.
Loss of heterozygosity (LOH) of the wild-type allele by structural chromosome aberrations (SCAs), homologous mitotic recombination (HMR) or intra-chromosomal (deletion/amplification) recombination (ICR) plays a crucial role in multistage carcinogenesis. We describe here an in vivo system, enabling the detection of all three chromosome breakage-related events in the same genetic experiment, with eye tissue of Drosophila as targets. This modification of the white/white(+) system enables to measure, simultaneously, HMR and ICR on the X-chromosome, and loss of a ring-shaped X-chromosome, utilizing the eye color gene white. Optimal conditions for the detection and quantification of SCAs (ring-X loss) compared to HMR are discussed in detail. Emerging new techniques comprise the parallel detection of HMR on chromosomes X and 3, using the tumor suppressor gene warts in addition to the X-linked marker white. Another modification of the white/white(+) system measures, again in parallel, HMR and chromosome duplication (non-disjunction).
Vogel
E W
EW
Leiden University Medical Centre, Department of Radiation Genetics and Chemical Mutagenesis, MGC Sylvius Laboratories, Wassenaarseweg 72, 2300 RA, Leiden, The Netherlands. vogel@mail.medfac.leidenuniv.nl
Nivard
M J
MJ
D000014:Abnormalities, Drug-Induced; D000818:Animals; D015197:Carcinogenicity Tests / Q000379:methods*; D002945:Cisplatin / Q000633:toxicity; D004249:DNA Damage; D004330:Drosophila / Q000187:drug effects*; D005123:Eye / Q000187:drug effects*; D019656:Loss of Heterozygosity; D008297:Male; D008741:Methyl Methanesulfonate / Q000633:toxicity; D009030:Mosaicism; D011995:Recombination, Genetic
D008741:Methyl Methanesulfonate; D002945:Cisplatin