title: Posttransfusion survival (24-hour) and hemolysis of previously frozen, deglycerolized RBCs after storage at 4 degrees C for up to 14 days in sodium chloride alone or sodium chloride supplemented with additive solutions.
abstract: BACKGROUND
  Previously frozen human RBCs currently are glycerolized and deglycerolized by the use of open systems that limit storage of the deglycerolized RBCs at 4 degrees C to only 24 hours.
  
  
  METHODS
  Healthy male volunteers who met AABB requirements for blood donors (n = 38) were studied. A volume of 450 mL of blood was collected into CPDA-1. The RBC concentrates were stored at 4 degrees C for 3 to 6 days before being frozen with 40-percent (wt/vol) glycerol and stored at -80 degrees C. The RBCs were deglycerolized, resuspended in 0.9-percent sodium chloride and 0.2-percent glucose (SG) solution or SG solution supplemented with AS-1, AS-3, or AS-5, and stored in the resuspension medium at 4 degrees C for 14 days.
  
  
  RESULTS
  The mean +/- SD freeze-thaw-wash process recovery was 90.0 +/- 4.0 percent for all 38 units. The mean 24-hour posttransfusion survival value was 79 percent for deglycerolized RBC stored at 4 degrees C for 7 days in SG alone, SG plus AS-3, or SG plus AS-5. Deglycerolized RBC that were stored at 4 C for 14 days in SG supplemented with AS-1, AS-3, or AS-5 had a mean 24-hour posttransfusion survival of 74 percent. After 7 days of storage of deglycerolized RBCs in SG alone, the mean hemolysis was 3. 7 percent. After 14 days of storage of deglycerolized RBCs in SG supplemented with AS-1, AS-3, or AS-5, the mean hemolysis was 2.5 percent.
  
  
  CONCLUSIONS
  The levels of hemolysis did not correlate with the 24-hour posttransfusion survival values.
authors: Naval Blood Research Laboratory, Boston University School of Medicine, Boston, Massachusetts 02118, USA. navblood@bu.edu
authors: C R
authors: CR
authors: Valeri
authors: L E
authors: LE
authors: Pivacek
authors: G P
authors: GP
authors: Cassidy
authors: G
authors: G
authors: Ragno
mesh_terms: D000225:Adenine / Q000494:pharmacology*; D001793:Blood Preservation; D001803:Blood Transfusion; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000378:metabolism*; D004905:Erythrocyte Aging / Q000502:physiology*; D004912:Erythrocytes / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology*; D005990:Glycerol / Q000378:metabolism*; D006461:Hemolysis; D006801:Humans; D008297:Male; D008353:Mannitol / Q000494:pharmacology*; D012965:Sodium Chloride / Q000494:pharmacology*; D013997:Time Factors
chemical_list: C037839:ADSOL; D003451:Cryoprotective Agents; D008353:Mannitol; D012965:Sodium Chloride; D005947:Glucose; D000225:Adenine; D005990:Glycerol