Feather keratin hydrolysis by a Vibrio sp. strain kr2.
The aim of the study was to characterize feather-degrading bacteria isolated from poultry industry waste. A Vibrio sp. strain kr2 producing a high keratinolytic activity when cultured on native feather-containing broth was isolated. The bacterium grew with an optimum at pH 6.0 and 30 degrees C, where maximum featherdegrading activity was also observed. Keratinase production was similar at both 25 and 30 degrees C, while the maximum concentration of soluble protein was reached at 30 degrees C. Reduction of disulphide bridges was also observed, increasing with cultivation time. The keratinase of strain kr2 was active on azokeratin, azocasein, benzoyl-arginine-p-nitroanilide and Ala-Ala-p-nitroanilide as substrates. The amino acid composition of the feather hydrolysate was determined, presenting similarities with that reported for feather lysate, feather meal and raw feathers. A novel feather-degrading bacterium was isolated and characterized, showing high keratinolytic activity. Complete feather degradation was achieved during cultivation. Strain kr2 shows potential for use for biotechnological processes involving keratin hydrolysis.
Sangali
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Departamento de Ciência de Alimentos, ICTA, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Brandelli
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D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001586:Benzoylarginine Nitroanilide / Q000737:chemistry; D002364:Caseins / Q000737:chemistry; D005241:Feathers / Q000737:chemistry / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007220:Industrial Waste; D007633:Keratins / Q000737:chemistry*; D010088:Oxidoreductases / Q000737:chemistry; D050862:Oxidoreductases Acting on Sulfur Group Donors; D010447:Peptide Hydrolases / Q000737:chemistry; D011200:Poultry / Q000382:microbiology; D013696:Temperature; D014733:Vibrio / Q000201:enzymology* / Q000302:isolation & purification
D000596:Amino Acids; D002364:Caseins; D007220:Industrial Waste; C026018:azocasein; D007633:Keratins; D001586:Benzoylarginine Nitroanilide; D010088:Oxidoreductases; D050862:Oxidoreductases Acting on Sulfur Group Donors; D010447:Peptide Hydrolases; C035855:keratinase