Isolation of 5 alpha- and 5 beta-dihydrorubrosterone from Silene otites L. (Wib).
5 alpha-Dihydrorubrosterone (2 beta, 3 beta, 14 alpha, 17 beta-tetrahydroxy-5 alpha-androst-7-ene-6-one), a new 19-carbon 5 alpha-ecdysteroid, was isolated together with its 5 beta counterpart from the aerial parts of Silene otites L. (Wib.) (Caryophyllaceae) by a combination of solvent partition, low-pressure column chromatography, thin-layer chromatography (normal-phase and reversed-phase) and finally HPLC. Mass spectrometry and nuclear magnetic resonance spectroscopic procedures were used for compound characterization.
Báthori
M
M
Department of Pharmacognosy, Albert Szent-Györgyi Medical University, H-6701 Szeged, Eötvös u. 6, P.O. Box 121, Hungary. bathori@pharma.szote.u-szeged.hu
Girault
J P
JP
Máthé
I
I
Lafont
R
R
D015068:17-Ketosteroids / Q000302:isolation & purification*; D000732:Androstanols / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000379:methods; D009682:Magnetic Resonance Spectroscopy; D019684:Magnoliopsida / Q000737:chemistry*; D013058:Mass Spectrometry
D015068:17-Ketosteroids; C418023:5-dihydrorubrosterone; D000732:Androstanols