title: Centromeric heterochromatin in the cattle rob(1;29) translocation: alpha-satellite I sequences, in-situ MspI digestion patterns, chromomycin staining and C-bands.
abstract: The centromeric regions and alpha-satellite I sequence were studied on chromosomes 1, 29 and the rob(1;29) translocation in a Portuguese breed of cattle, Barrosa, carrying the translocation. Rob(1;29) centromeric regions showed heterochromatic bands with propidium iodide but, unlike the acrocentric autosomes, no strong centromeric bands were revealed with chromomycin A3. An alpha-satellite I sequence was not found at the centromeres of the X, Y and rob(1;29) chromosomes in the breed, although it was present at the centromeres of all acrocentric chromosomes including 1 and 29. Restriction enzyme banding with MspI revealed polymorphisms between different rob(1;29) chromosomes in both centromeric and intercalary regions. The data show that the centromeric region of the rob(1;29) chromosome has lost the alpha-satellite I sequences, while retaining other heterochromatin, and suggest that this important and widespread translocation has occurred multiple times.
authors: Department of Genetics and Biotechnology, ICETA-UTAD, University of Trás-os-Montes and Alto Douro,Vila Real, Portugal.
authors: R
authors: R
authors: Chaves
authors: J S
authors: JS
authors: Heslop-Harrsion
authors: H
authors: H
authors: Guedes-Pinto
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics*; D002503:Centromere / Q000235:genetics / Q000648:ultrastructure; D014128:Chromomycin A3; D002871:Chromosome Banding; D004276:DNA, Satellite / Q000235:genetics*; D019003:Deoxyribonuclease HpaII / Q000378:metabolism; D005456:Fluorescent Dyes; D006570:Heterochromatin / Q000648:ultrastructure; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D011110:Polymorphism, Genetic; D014178:Translocation, Genetic
chemical_list: D004276:DNA, Satellite; D005456:Fluorescent Dyes; D006570:Heterochromatin; D014128:Chromomycin A3; D019003:Deoxyribonuclease HpaII