title: Internal support of tissue-engineered cartilage.
abstract: BACKGROUND
  Auricles previously created by tissue engineering in nude mice used a biodegradable internal scaffold to maintain the desired shape of an ear. However, the biodegradable scaffold incited a compromising inflammatory response in subsequent experiments in immunocompetent animals.
  
  
  OBJECTIVE
  To test the hypothesis that tissue-engineered autologous cartilage can be bioincorporated with a nonreactive, permanent endoskeletal scaffold.
  
  
  METHODS
  Auricular elastic cartilage was harvested from Yorkshire swine. The chondrocytes were isolated and suspended into a hydrogel (Pluronic F-127) at a cell concentration of 5 x 10(7) cells/mL. Nonbiodegradable endoskeletal scaffolds were formed with 1 of 5 polymers: (1) high-density polyethylene, (2) soft acrylic, (3) polymethylmethacrylate, (4) extrapurified Silastic, and (5) conventional Silastic. Three groups were studied: (1) a control group using only the 5 polymers, (2) the 5 polymers enveloped by Pluronic F-127 only, and (3) the implants coated with Pluronic F-127 seeded with chondrocytes. All constructs were implanted subdermally; implants containing cells were implanted into the same animal from which the cells had been islolated. The implants were harvested after 8 weeks of in vivo culture and histologically analyzed.
  
  
  RESULTS
  Only implants coated by hydrogel plus cells generated healthy new cartilage. With 3 polymers (high-density polyethylene, acrylic, and extrapurified Silastic), the coverage was nearly complete by elastic cartilage, with minimal fibrocartilage and minimal to no inflammatory reaction. The Food and Drug Administration-approved conventional Silastic implants resulted in fragments of fibrous tissue mixed with elastic cartilage plus evidence of chronic inflammation. The polymethylmethacrylate implant was intermediate in the amount of cartilage formed and degree of inflammation.
  
  
  CONCLUSIONS
  This pilot technique combining tissue-engineered autologous elastic cartilage with a permanent biocompatible endoskeleton demonstrated success in limiting the inflammatory response to the scaffold, especially to high-density polyethylene, acrylic, and extrapurified Silastic. This model facilitates the potential to generate tissue of intricate shape, such as the human ear, by internal support. Arch Otolaryngol Head Neck Surg. 2000;126:1448-1452
authors: Department of Otolaryngology, Massachusetts Eye and Ear Infirmary, 243 Charles St, Boston, MA 02114, USA.
authors: C A
authors: CA
authors: Arévalo-Silva
authors: R D
authors: RD
authors: Eavey
authors: Y
authors: Y
authors: Cao
authors: M
authors: M
authors: Vacanti
authors: Y
authors: Y
authors: Weng
authors: C A
authors: CA
authors: Vacanti
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D019902:Chondrocytes / Q000637:transplantation*; D020099:Coated Materials, Biocompatible / Q000008:administration & dosage; D003599:Cytoskeleton; D004129:Dimethylpolysiloxanes / Q000008:administration & dosage; D004425:Ear Cartilage; D006801:Humans; D020100:Hydrogels / Q000008:administration & dosage; D007249:Inflammation; D008297:Male; D010865:Pilot Projects; D020442:Poloxamer / Q000008:administration & dosage; D011095:Polyethylenes / Q000008:administration & dosage; D011109:Polymethacrylic Acids / Q000008:administration & dosage; D019736:Prostheses and Implants; D019919:Prosthesis Implantation; D012828:Silicones / Q000008:administration & dosage; D013552:Swine; D013997:Time Factors; D014182:Transplantation, Autologous
chemical_list: D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D004129:Dimethylpolysiloxanes; D020100:Hydrogels; D011095:Polyethylenes; D011109:Polymethacrylic Acids; D012828:Silicones; D020442:Poloxamer; C030613:polymethacrylic acid; C013830:baysilon