Identification of a nitric oxide-regulated zinc finger containing transcription factor using motif-directed differential display.
We report here the isolation of human zinc finger 2 (HZF2), a putative zinc-finger transcription factor, by motif-directed differential display of mRNA extracted from histamine-stimulated human vein endothelial cells. The expression of HZF2 mRNA in venous endothelial cells was verified by Northern blot analysis, which also revealed an enrichment of HZF2 mRNA in lymphocytes and monocytes. Histamine induced a time- and concentration-dependent upregulation of HZF2 level with a 6-fold peak increase of mRNA at 30 min. HZF2 upregulation was abolished by different NOS isozyme inhibitors. Guanylate cyclase inhibition resulted in a significant decrease of HZF2 expression. These observations indicate HZF2 as a potentially interesting new target for studies concerning rapid NO-mediated gene regulation.
Schäfer
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U
Biochemical and Experimental Division, II. Department of Surgery, University of Cologne, Ostmerheimer Str. 200, 51109 Cologne, Germany. u.schaefer@uni-koeln.de
Schneider
A
A
Neugebauer
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E
D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D004730:Endothelium, Vascular / Q000378:metabolism; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006162:Guanylate Cyclase / Q000037:antagonists & inhibitors / Q000378:metabolism; D006632:Histamine / Q000494:pharmacology; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D051741:Kruppel-Like Transcription Factors; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D020799:Pancrelipase / Q000378:metabolism; D010920:Placenta / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D014157:Transcription Factors / Q000737:chemistry*; D015854:Up-Regulation; D016335:Zinc Fingers / Q000235:genetics*
D004268:DNA-Binding Proteins; D004791:Enzyme Inhibitors; D007527:Isoenzymes; D051741:Kruppel-Like Transcription Factors; D012333:RNA, Messenger; D014157:Transcription Factors; C118913:ZNF2 protein, human; D009569:Nitric Oxide; D020799:Pancrelipase; D006632:Histamine; D019001:Nitric Oxide Synthase; D006162:Guanylate Cyclase