Ordered recruitment of chromatin modifying and general transcription factors to the IFN-beta promoter.
Here, we show that the IFN-beta enhanceosome activates transcription by directing the ordered recruitment of chromatin modifying and general transcription factors to the IFN-beta promoter. The enhanceosome is assembled in the nucleosome-free enhancer region of the IFN-beta gene, leading to the modification and remodeling of a strategically positioned nucleosome that masks the TATA box and the start site of transcription. Initially, the GCN5 complex is recruited, which acetylates the nucleosome, and this is followed by recruitment of the CBP-PolII holoenzyme complex. Nucleosome acetylation in turn facilitates SWI/SNF recruitment by CBP, resulting in chromatin remodeling. This program of recruitment culminates in the binding of TFIID to the promoter and the activation of transcription.
Agalioti
T
T
Department of Biochemistry and Molecular Biophysics, Columbia University, 630 West 168th Street, New York, NY 10032, USA.
Lomvardas
S
S
Parekh
B
B
Yie
J
J
Maniatis
T
T
Thanos
D
D
D000107:Acetylation; D000123:Acetyltransferases / Q000378:metabolism; D050882:CREB-Binding Protein; D002843:Chromatin / Q000378:metabolism*; D004257:DNA Polymerase II / Q000378:metabolism; D029721:Drosophila Proteins; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D006657:Histones / Q000378:metabolism; D016899:Interferon-beta / Q000235:genetics*; D009687:Nuclear Proteins / Q000378:metabolism; D009707:Nucleosomes; D011401:Promoter Regions, Genetic; D016601:RNA-Binding Proteins; D017412:Ribonucleoprotein, U1 Small Nuclear / Q000378:metabolism; D016385:TATA Box; D015534:Trans-Activators / Q000378:metabolism; D035362:Transcription Factor TFIID; D014157:Transcription Factors / Q000378:metabolism*; D019473:Transcription Factors, TFII / Q000378:metabolism
D002843:Chromatin; D029721:Drosophila Proteins; D006657:Histones; D009687:Nuclear Proteins; D009707:Nucleosomes; D016601:RNA-Binding Proteins; D017412:Ribonucleoprotein, U1 Small Nuclear; D015534:Trans-Activators; D035362:Transcription Factor TFIID; D014157:Transcription Factors; D019473:Transcription Factors, TFII; C089969:snf protein, Drosophila; D016899:Interferon-beta; D000123:Acetyltransferases; D050882:CREB-Binding Protein; D004257:DNA Polymerase II