Detection and correction of a migration anomaly on a 310 genetic analyzer.
During STR analysis on the 310 Genetic Analyzer, retarded migration of GS500ROX size standards and alleles in some samples was observed. The contribution of reagents, capillary and performance optimized polymer POP 4 to the observed anomaly was experimentally eliminated. Variation in electrophoresis temperature between 55 degrees C and 65 degrees C did not alter the rate of migration of GX500ROX size standard and sample alleles. An eroded connector for the cathode mounted on the heat plate assembly caused the abnormal migration. Hence, it is important to verify the mobility of all fragments in the size standard for each sample to avoid any erroneous allele calls by the automated data analysis software.
Shewale
J G
JG
ReliaGene Technologies, Inc., New Orleans, LA 70123, USA.
Richey
S L
SL
Sinha
S K
SK
D016172:DNA Fingerprinting; D019075:Electrophoresis, Capillary; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D016133:Polymerase Chain Reaction; D012015:Reference Standards; D015203:Reproducibility of Results; D020080:Tandem Repeat Sequences / Q000235:genetics*