Production and purification of recombinant human alpha 2C2 adrenergic receptor using Saccharomyces cerevisiae.
The objective is to generate milligram quantities of recombinant human alpha 2C2 adrenergic receptor for X-ray crystallographic studies. It has been cloned in Saccharomyces cerevisiae, and the production level is at best about 13 pmol/mg of membrane protein, as estimated by radio-ligand binding assay. The receptor is solubilized with sucrose monolaurate followed by immunoaffinity purification and reconstitution into phospholipid vesicles. The efficiency of solubilization and immuno-purification are 60% and 91%, respectively.
Kapat
A
A
Institute of Biotechnology, University of Helsinki, Finland.
Jaakola
V P
VP
Heimo
H
H
Liitti
S
S
Heikinheimo
P
P
Glumoff
T
T
Goldman
A
A
D006801:Humans; D008967:Molecular Biology / Q000191:economics; D018341:Receptors, Adrenergic, alpha-2 / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism
C547774:ADRA2C protein, human; D018341:Receptors, Adrenergic, alpha-2; D011994:Recombinant Proteins