title: A generalized fecal glucocorticoid assay for use in a diverse array of nondomestic mammalian and avian species.
abstract: Noninvasive fecal glucocorticoid analysis has tremendous potential as a means of assessing stress associated with environmental disturbance in wildlife. However, interspecific variation in excreted glucocorticoid metabolites requires careful selection of the antibody used in their quantification. We compared four antibodies for detecting the major fecal cortisol metabolites in yellow baboons following (3)H cortisol administration, ACTH challenge, and HPLC separation of fecal glucocorticoid metabolites. The most effective antibody (ICN corticosterone RIA; Cat. No. 07-120102) demonstrated relatively high cross-reactivities to the major cortisol metabolites present in feces during peak excretion, following both radiolabel infusion and ACTH challenge. This same antibody also detected increased fecal glucocorticoid metabolites after ACTH administration in the African elephant, black rhinoceros, Roosevelt elk, gerenuk, scimitar-horned oryx, Alaskan sea otter, Malayan sun bear, cheetah, clouded leopard, longtailed macaque, and northern spotted owl. Results suggest that (1) fecal glucocorticoid assays reliably detect endogenous changes in adrenal activity of a diverse array of species and (2) where comparisons were made, the ICN corticosterone antibody generally was superior to other antibodies for measuring glucocorticoid metabolites in feces.
authors: Division of Reproductive Endocrinology, University of Washington School of Medicine, Seattle, Washington 98195, USA. wassers@u.washington.edu
authors: S K
authors: SK
authors: Wasser
authors: K E
authors: KE
authors: Hunt
authors: J L
authors: JL
authors: Brown
authors: K
authors: K
authors: Cooper
authors: C M
authors: CM
authors: Crockett
authors: U
authors: U
authors: Bechert
authors: J J
authors: JJ
authors: Millspaugh
authors: S
authors: S
authors: Larson
authors: S L
authors: SL
authors: Monfort
mesh_terms: D000324:Adrenocorticotropic Hormone; D000818:Animals; D000835:Animals, Wild; D000866:Antelopes; D000918:Antibody Specificity; D001717:Birds; D002335:Carnivora; D002851:Chromatography, High Pressure Liquid; D004606:Elephants; D004777:Environment; D005243:Feces / Q000737:chemistry*; D005260:Female; D005938:Glucocorticoids / Q000032:analysis*; D006854:Hydrocortisone / Q000032:analysis / Q000378:metabolism; D008251:Macaca; D008297:Male; D008322:Mammals; D010041:Otters; D010215:Papio; D011863:Radioimmunoassay; D013045:Species Specificity; D013312:Stress, Physiological / Q000209:etiology / Q000378:metabolism; D020305:Strigiformes; D014316:Tritium; D001503:Ursidae
chemical_list: D005938:Glucocorticoids; D014316:Tritium; D000324:Adrenocorticotropic Hormone; D006854:Hydrocortisone