The hDLG-associated protein DAP interacts with dynein light chain and neuronal nitric oxide synthase.
BACKGROUND
Postsynaptic density (PSD)-95 interacts with and mediates clustering of the N-methyl-D-aspartate-receptors (NMDA-R). PSD-95 also interacts with the hDLG-associated protein DAP, which is also called Synapse-associated protein 90-associated protein (SAPAP), and Guanylate kinase-associated protein (GKAP).


RESULTS
DAP interacted directly with the dynein light chain (DLC) family of proteins. DLC was contained in the NMDA-R-PSD-95-DAP-neuronal nitric oxide synthase (nNOS) complex. Furthermore, DAP interacted with nNOS and recruited it into the Triton X-100-insoluble fraction of transfected cells.


CONCLUSIONS
DAP interacts directly with DLC and nNOS, and links these proteins to the NMDA-R-PSD-95 complex.
Haraguchi
K
K
Laboratory of Molecular and Genetic Information, Institute for Molecular and Cellular Biosciences, The University of Tokyo, Japan.
Satoh
K
K
Yanai
H
H
Hamada
F
F
Kawabuchi
M
M
Akiyama
T
T
D000818:Animals; D000918:Antibody Specificity; D001665:Binding Sites / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D002460:Cell Line; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D000076122:Disks Large Homolog 4 Protein; D029721:Drosophila Proteins; D004398:Dyneins; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D046911:Macromolecular Substances; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D052248:Nitric Oxide Synthase Type I; D017830:Octoxynol; D011233:Precipitin Tests; D011485:Protein Binding / Q000235:genetics; D020033:Protein Isoforms / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism; D000074407:SAP90-PSD95 Associated Proteins; D020798:Two-Hybrid System Techniques
D002352:Carrier Proteins; C000614187:DLGAP1 protein, human; D018076:DNA, Complementary; D000076122:Disks Large Homolog 4 Protein; C501449:Dlg4 protein, rat; D029721:Drosophila Proteins; D047908:Intracellular Signaling Peptides and Proteins; D046911:Macromolecular Substances; C000614192:Mars protein, Drosophila; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D020033:Protein Isoforms; D016194:Receptors, N-Methyl-D-Aspartate; D000074407:SAP90-PSD95 Associated Proteins; C033652:postsynaptic density proteins; D017830:Octoxynol; C496320:NOS1 protein, human; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; C496322:Nos1 protein, rat; D004398:Dyneins