title: Scots pine expresses short-root-specific peroxidases during development.
abstract: Nine short-root-specific proteins from Scots pine (Pinus sylvestris L.) detected and isolated as individual spots by 2D-PAGE were identified. The similar peptide mass maps obtained for all nine polypeptide spots together with lectin-blotting results suggest that they represent forms of the same modified protein. N-Terminal sequence analysis of two of the peptides showed high similarity to peroxidases. RT-PCR with oligonucleotide primers corresponding to determined peptide sequences and conserved regions in plant peroxidases led to isolation of Psyp1 cDNA which is most abundantly expressed in short roots. Psyp1 is the first peroxidase cDNA to be isolated from the genus Pinus. It encodes a 363-amino-acid class-III peroxidase with a calculated molecular mass of 35.7 kDa and theoretical pI of 4.74. The predicted PSYP1 amino-acid sequence is grouped with other class-III peroxidases in phylogenetic analyses, but it has a unique amino-acid sequence which may be associated with its function in short roots or with its phylogenetic group. The presence of a signal sequence for extracellular transport indicates that PSYP1 belongs to the group of secreted class-III peroxidases. The presence of 10 tyrosine residues and putative auxin-binding regions in PSYP1 suggests that the function of the enzyme is associated with cell-wall formation in short roots. The downregulation of Psyp1 expression in symbiotic short roots hosting the ectomycorrhizal fungus Suillus bovinus is perhaps related to the change in cell-wall structure necessary for ectomycorrhizal development.
authors: Department of Biosciences, Division of Plant Physiology, University of Helsinki, Finland. mika.tarkka@helsinki.fi
authors: M T
authors: MT
authors: Tarkka
authors: T A
authors: TA
authors: Nyman
authors: N
authors: N
authors: Kalkkinen
authors: M
authors: M
authors: Raudaskoski
mesh_terms: D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins; D032462:Cycadopsida / Q000201:enzymology* / Q000235:genetics; D018076:DNA, Complementary / Q000032:analysis; D015870:Gene Expression; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D018517:Plant Roots / Q000201:enzymology / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000145:classification / Q000235:genetics / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D018076:DNA, Complementary; C410510:SHORT ROOT protein, Arabidopsis; D014157:Transcription Factors; D010544:Peroxidases