title: Thymic stress in artificially reared and maternally reared rat pups.
abstract: It is well documented that the hypothalamic-pituitary-adrenal (HPA) axis influences immunological responses to stress. Maternal factors have been shown to be necessary for appropriate modulation of the HPA axis in the developing rat. The purpose of this study was to determine whether artificially reared (AR) infant rat pups (a procedure whereby infant rats are gastrostomized and reared independently of maternal factors) have an altered function of the HPA axis in terms of thymocyte apoptosis (programmed cell death) and other indices of thymic stress. AR and maternally reared (MR) Long-Evans rat pups were randomized to control, fasted, stressed, and fasted+stressed treatment groups, as well as an unhandled, MR naive group that served as a baseline control. AR rat pups were significantly heavier than MR (p<0.001). AR rat pups had significantly lighter thymuses than did the MR pups (p<0.001) and fasted pups had significantly lighter thymuses than unfasted pups, regardless of whether they were in the AR or MR condition (p<0.005). AR pups had significantly lower thymic cell numbers and a greater percent of necrotic cells than did MR pups. There were no significant effects of rearing condition on the percent of apoptotic thymocytes. The thymocyte alterations observed in this study between the two rearing conditions suggest that AR reduces thymic weight and cell numbers, which may have consequences for the development of adult cellular immunity.
authors: Department of Health Studies and Gerontology, Faculty of Applied Health Sciences, University of Waterloo, Waterloo, Ontario, Canada, N2L 3G1. lhgoetz@healthy.uwaterloo.ca
authors: L
authors: L
authors: Hoffman-Goetz
authors: J
authors: J
authors: Slivchak
authors: J
authors: J
authors: Konyer
authors: G
authors: G
authors: Ward
mesh_terms: D000818:Animals; D000833:Animals, Suckling; D017209:Apoptosis; D001835:Body Weight; D002452:Cell Count; D002470:Cell Survival; D004750:Enteral Nutrition; D005508:Food Deprivation / Q000502:physiology; D005774:Gastrostomy; D007441:Intubation, Gastrointestinal; D007774:Lactation; D008426:Maternal Deprivation; D009336:Necrosis; D009929:Organ Size; D051381:Rats; D020318:Rats, Long-Evans; D013312:Stress, Physiological / Q000276:immunology; D013950:Thymus Gland / Q000166:cytology* / Q000254:growth & development / Q000276:immunology