title: Molecular cloning and relative tissue expression of decorin and lumican in embryonic quail cornea.
abstract: We have cloned and sequenced the cDNAs for quail cornea proteoglycan core proteins, decorin and lumican. Comparison of deduced amino acid sequences shows that two of five amino acid differences in the mature protein between quail and chick decorin, and two of three for lumican, are non-conservative. Ribonuclease protection assay of Day 16 embryonic quail tissues reveals that decorin and lumican are most highly expressed in cornea, and that both are also highly expressed at approximately equal levels in most other tissues. Decorin is highly expressed in sclera and sternum, whereas lumican is expressed in these tissues, as well as in liver, at very low levels. Both decorin and lumican are expressed at lowest levels in brain.
authors: Division of Biology, Kansas State University, Manhattan, KS, 66506-4901, USA.
authors: L M
authors: LM
authors: Corpuz
authors: J R
authors: JR
authors: Dunlevy
authors: J R
authors: JR
authors: Hassell
authors: A H
authors: AH
authors: Conrad
authors: G W
authors: GW
authors: Conrad
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D011508:Chondroitin Sulfate Proteoglycans / Q000235:genetics*; D003001:Cloning, Molecular; D003315:Cornea / Q000196:embryology* / Q000378:metabolism; D018076:DNA, Complementary; D058575:Decorin; D016326:Extracellular Matrix Proteins; D005136:Eye Proteins / Q000235:genetics*; D015870:Gene Expression; D007632:Keratan Sulfate / Q000235:genetics*; D000071234:Lumican; D008969:Molecular Sequence Data; D011509:Proteoglycans / Q000235:genetics*; D011784:Quail
chemical_list: D011508:Chondroitin Sulfate Proteoglycans; D018076:DNA, Complementary; D058575:Decorin; D016326:Extracellular Matrix Proteins; D005136:Eye Proteins; D000071234:Lumican; D011509:Proteoglycans; D007632:Keratan Sulfate