Non-competitive inhibition of kainate-induced currents by diethylstilbestrol in acutely isolated mouse CA1 hippocampal neurons.
The effect of a synthetic estrogen, diethylstilbestrol (DES), on kainate-induced currents was investigated in the hippocampal CA1 pyramidal neurons acutely dissociated from the mice using the nystatin-perforated patch-clamp recording configuration under voltage-clamp conditions. DES inhibited the current evoked by 100 microM kainate in a concentration-dependent manner with a half-maximum inhibitory concentration of 8.8 microM. The action of DES was voltage-independent. Since DES produced a suppression of the maximum response of the kainate concentration-response curve, the inhibition by DES of the kainate-induced current appears to be non-competitive.
Ishibashi
H
H
Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
Okuya
S
S
Shimada
H
H
Takahama
K
K
D000818:Animals; D004054:Diethylstilbestrol / Q000494:pharmacology*; D004347:Drug Interactions; D004594:Electrophysiology; D004968:Estrogens, Non-Steroidal / Q000494:pharmacology; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects* / Q000502:physiology; D066298:In Vitro Techniques; D007608:Kainic Acid / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D051379:Mice; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D018408:Patch-Clamp Techniques
D004968:Estrogens, Non-Steroidal; D018690:Excitatory Amino Acid Agonists; D004054:Diethylstilbestrol; D007608:Kainic Acid