title: Alteration of 6-phosphofructo-1-kinase subunits during neonatal maturation of the rat cochlear cells.
abstract: During postnatal development of rat cochlear cells and the onset of hearing (10-23 days), the increasing endocochlear potential and energy requirements are largely provided by increased glucose utilization. It is well established that the ability of maturing rat tissues to use glucose is directly related to alteration of 6-phosphofructo-1-kinase (PFK) subunits. To gain insight into the alteration of PFK subunit levels in the cochlea from 6 to 60 days of age, PFK subunit types were measured in sections of paraffin-embedded temporal bone using IgG specific for each type of PFK subunit and quantified by computer image analysis. Although the L-type and C-type subunits did not exhibit statistically significant changes in the cochlear structures during maturation, the levels of M-type subunit in the stria vascularis cells, spiral ligament cell types I, II, and III, outer hair cells, inner hair cells, and support cells significantly increased. Also, the type IV and V spiral ligament fibrocytes during this period did not exhibit significant alterations of the M-type subunit. These data suggest that during neonatal development of the cochlear, the elevated levels of the M-type subunit are associated with increased glucose utilization and the onset of hearing.
authors: Department of Pharmacology, Division of Otolaryngology, Southern Illinois University School of Medicine, Springfield 62794-9629, USA.
authors: G
authors: G
authors: Armour
authors: Y
authors: Y
authors: Mhaskar
authors: L
authors: L
authors: Rybak
authors: G
authors: G
authors: Dunaway
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D003051:Cochlea / Q000166:cytology / Q000201:enzymology* / Q000254:growth & development*; D004734:Energy Metabolism; D005947:Glucose / Q000378:metabolism; D006199:Hair Cells, Auditory, Inner / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D018072:Hair Cells, Auditory, Outer / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D006309:Hearing / Q000502:physiology; D007150:Immunohistochemistry; D010732:Phosphofructokinase-1 / Q000737:chemistry / Q000378:metabolism*; D021122:Protein Subunits; D051381:Rats; D011916:Rats, Inbred F344; D013316:Stria Vascularis / Q000166:cytology / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D021122:Protein Subunits; D010732:Phosphofructokinase-1; D005947:Glucose