Simian virus 40 is present in human lymphomas and normal blood.
Many independent studies have demonstrated Simian virus 40 (SV40) in normal and neoplastic human tissues. Clonal integration of virus in the DNA of several thyroid and bone tumors suggests a direct role for SV40 in some cancers. However, in most cases the role of SV40 remains unclear. This study determined the presence of SV40, by amplification followed by hybridization, in 266 normal and neoplastic blood and lymphoid samples. Amplification detected SV40 in 14% of non-autoimmune deficiency syndrome (AIDS) lymphomas, 28% of AIDS related lymphoma and 16% of peripheral blood lymphocytes from non-cancerous patients. No SV40 was detected in leukemia samples. Direct Southern blotting of SV40+ samples detected no virus, consistent with less than one viral genome in ten cells. Sequence analysis of SV40 in blood and lymphoid samples found sequences distinct from laboratory strains of SV40. The presence of limited quantities of SV40 in a small proportion of both normal and neoplastic tissues is suggestive of an adventitious presence with no apparent direct role in blood and lymphoid cancers.
David
H
H
Division of Hematology/Oncology, Department of Medicine, UCLA School of Medicine, Cedars-Sinai Research Institute, 8700 Beverly Boulevard, Davis 5019, Los Angeles, CA 90048, USA.
Mendoza
S
S
Konishi
T
T
Miller
C W
CW
D000952:Antigens, Polyomavirus Transforming / Q000737:chemistry; D015139:Blotting, Southern; D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D008223:Lymphoma / Q000821:virology*; D016483:Lymphoma, AIDS-Related / Q000821:virology; D016133:Polymerase Chain Reaction; D013539:Simian virus 40 / Q000145:classification / Q000302:isolation & purification*; D014766:Viremia / Q000821:virology*
D000952:Antigens, Polyomavirus Transforming; D004279:DNA, Viral