Application of capillary electrophoresis to the analysis of soluble chromatin.
Capillary electrophoresis (CE) has been applied to study DNA-protein complexes using as the test system soluble chromatin from chicken erythrocytes and rapidly proliferated cultured Chinese hamster fibroblast-like cells B11-dii-FAF-28. Separation was performed with home-made CE apparatus, using a regulated high-voltage power supply, UV-detector and fused silica capillaries with inner diameter 75 microm. The heterogeneity of nucleosomal particles with different DNA lengths after micrococcal nuclease digestion was detected.
Asatiani
N V
NV
Institute of Physics, Georgian Academy of Sciences, Tamarashvili str. 6, Tbilisi, Georgia.
Abuladze
M K
MK
Birkaya
B G
BG
Sapojnikova
N A
NA
Tsibakhashvili
N Y
NY
Mosulishvili
L M
LM
D000818:Animals; D002460:Cell Line; D002645:Chickens; D002843:Chromatin / Q000737:chemistry*; D006224:Cricetinae; D003412:Cricetulus; D019075:Electrophoresis, Capillary / Q000379:methods*; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet
D002843:Chromatin