Simultaneous micellar electrokinetic chromatographic determination of isomeric fatty acid hydroperoxides and corresponding hydroxy fatty acids.
The high selectivity and efficiency of micellar electrokinetic chromatography with a borax-sodium dodecylsulfate (SDS) or meglumin-SDS buffer make possible the rapid separation of hydroperoxy and hydroxy fatty acids and the non-oxidised unsaturated fatty acids from which they are derived. Nearly all the isomers of the hydroperoxides and hydroxy fatty acids derived from oleic, linoleic, alpha- and gamma-linolenic and arachidonic acids can be determined both qualitatively and quantitatively within ca. 10 min. The system has as many as 1 x 10(6) theoretical plates, and the detection limits with UV diode array detection at 195 or 234 nm are in the micromolar range.
Melchior
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Department of Analytical Chemistry. Bergische Universität-Gesamthochschule Wuppertal, Germany.
Gäb
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S
D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D005227:Fatty Acids / Q000032:analysis*; D007536:Isomerism; D008054:Lipid Peroxides / Q000032:analysis*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D005227:Fatty Acids; D008054:Lipid Peroxides