Reaction monitoring of succinylation of collagen with matrix-assisted laser desorption/ionization mass spectrometry.
Succinylated collagen was synthesized by the reaction of collagen with succinic anhydride under basic conditions for one hour. Using the matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) technique, the reaction products were directly identified without multi-step separation processes. MALDI-MS monitored the reaction more accurately than the conventional method of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). From the change in molecular mass during the reaction, it is observed that about 28 succinyl groups are attached to the collagen strand.
Kim
S H
SH
Department of Chemistry, Soonchunhyang University, Asan, South Korea. shkim71@sch.ac.kr
Lee
J H
JH
Yun
S Y
SY
Yoo
J S
JS
Jun
C H
CH
Chung
K Y
KY
Suh
H
H
D000818:Animals; D002417:Cattle; D003094:Collagen / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013386:Succinates / Q000737:chemistry*
D013386:Succinates; D003094:Collagen