Cloning and expression of cDNAs encoding two enzymes of the MEP pathway in Catharanthus roseus.
Two periwinkle cDNAs (crdxr and crmecs) encoding enzymes of the non-mevalonate terpenoid pathway were characterized using reverse transcription-PCR strategy based on the design of degenerated oligonucleotides. The deduced amino acid sequence of crdxr is homologue to 1-deoxy-D-xylulose 5-phosphate reductoisomerases. Crmecs represents the first plant cDNA encoding a protein similar to the 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase from Escherichia coli. Expression of crdxr and crmecs genes was up-regulated in periwinkle cells producing monoterpenoid indole alkaloids. Involvement of the 2C-methyl-D-erythritol 4-phosphate pathway in alkaloid biosynthesis is discussed.
Veau
B
B
Laboratoire de Biologie Moléculaire et Biochimie végétale, EA2106, Faculté de Pharmacie, Université de Tours, 31 avenue Monge, 37200 Tours, France.
Courtois
M
M
Oudin
A
A
Chénieux
J C
JC
Rideau
M
M
Clastre
M
M
D019747:Aldose-Ketose Isomerases / Q000737:chemistry / Q000235:genetics*; D000470:Alkaloids; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry*; D004896:Erythritol / Q000031:analogs & derivatives / Q000378:metabolism*; D017343:Genes, Plant; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000737:chemistry / Q000235:genetics*; D009713:Nucleotidyltransferases / Q000737:chemistry / Q000235:genetics*; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013403:Sugar Phosphates / Q000378:metabolism*
C114232:2-C-methylerythritol 4-phosphate; D000470:Alkaloids; D018076:DNA, Complementary; D009097:Multienzyme Complexes; D013403:Sugar Phosphates; D010088:Oxidoreductases; C114231:1-deoxy-D-xylulose 5-phosphate reductoisomerase; C400183:4-diphosphocytidyl-2-C--methylerythritol synthase; D009713:Nucleotidyltransferases; D019747:Aldose-Ketose Isomerases; D004896:Erythritol