Effects of overexpression of the liver subunit of 6-phosphofructo-1-kinase on the metabolism of a cultured mammalian cell line.
Overexpression of the liver subunit of 6-phosphofructo-1-kinase in Chinese hamster ovary K1 cells was shown to increase the steady-state level of the enzyme's product, fructose 1, 6-bisphosphate, and to produce a small but significant decrease in the concentration of fructose 2,6-bisphosphate, which is an allosteric activator of the enzyme. However, overexpression of the enzyme had no effect on glycolytic flux under a variety of different substrate conditions. This latter observation is consistent with similar studies in fungi and in potato tubers which indicate that 6-phosphofructo-1-kinase has very little control over flux in glycolysis.
Urbano
A M
AM
Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Old Addenbrook's Site, Cambridge CB2 1GA, U.K.
Gillham
H
H
Groner
Y
Y
Brindle
K M
KM
D000494:Allosteric Regulation; D000818:Animals; D016466:CHO Cells; D002455:Cell Division; D006224:Cricetinae; D005635:Fructosediphosphates / Q000378:metabolism; D015870:Gene Expression; D005947:Glucose / Q000378:metabolism; D019298:Glucose-6-Phosphate / Q000378:metabolism; D006019:Glycolysis; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D010732:Phosphofructokinase-1 / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D021122:Protein Subunits; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D005635:Fructosediphosphates; D021122:Protein Subunits; D011994:Recombinant Proteins; D019298:Glucose-6-Phosphate; C027652:fructose 2,6-diphosphate; D010732:Phosphofructokinase-1; D005947:Glucose; C029063:fructose-1,6-diphosphate