Chromatographic and capillary electrophoretic methods for the analysis of nicotinic acid and its metabolites.
Methods for the assay of nicotinic acid (NiAc) and its metabolites in biological fluids using high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE) are reviewed. Most of the references cited in this review concern HPLC methods. A few CE methods that have been recently reported are also included. As these compounds are relatively polar and have a wide range of physico-chemical properties, the sample pre-treatment or clean-up process prior to analysis is included. Most HPLC methods using an isocratic elution system allow determination of a single or few metabolites, but gradient HPLC methods enable simultaneous determination of five to eight compounds. Simultaneous determination of NiAc including many metabolites in a single run can be achieved by CE. We also discuss the pharmacokinetics of NiAc and some of its metabolites.
Iwaki
M
M
Faculty of Pharmaceutical Sciences, Kinki University, Osaka, Japan.
Murakami
E
E
Kakehi
K
K
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D009525:Niacin / Q000032:analysis* / Q000378:metabolism / Q000493:pharmacokinetics
D009525:Niacin