A Schistosoma mansoni 62-kDa band is identified as an irradiated vaccine T-cell antigen and characterized as calreticulin.
The Schistosoma mansoni soluble adult worm antigen (SAWA) bands of 62/60 kDa were found to contain immunodominant T-cell immunogen(s) in irradiated cercariae-immunized Swiss and C57BL/6 mice. In the present study, spleen T cells of BALB/c mice immunized twice with ultraviolet light-irradiated cercariae proliferated and produced interleukin 4 in response to the 62/60-kDa SAWA bands in T-cell western assays. To characterize the 62/60-kDa bands, an adult S. mansoni worm cDNA expression library constructed in lambdagt11 was immunoscreened with serum of mice immunized with the 62/60-kDa antigens, and the immunoreactive cDNA inserts were sequenced. Purified 62- and 60-kDa proteins were used for amino acid microsequencing and for immunization studies in Swiss, C57BL/6, and BALB/c mice and rabbits. Taken together, the data indicated that the 60-kDa molecules are poorly immunogenic in mice and rabbits, whereas the 62-kDa species identified as S. mansoni calreticulin, is a good T- and B-cell antigen and represents a potential vaccine candidate.
El Gengehi
N
N
Biomedical Research Centre for Infectious Diseases, the Egyptian Organization for Biological Products and Vaccines, Cairo.
El Ridi
R
R
Tawab
N A
NA
El Demellawy
M
M
Mangold
B L
BL
D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D037282:Calreticulin; D003001:Cloning, Molecular; D018984:Epitopes, T-Lymphocyte / Q000235:genetics / Q000276:immunology*; D005260:Female; D007114:Immunization / Q000379:methods; D016056:Immunodominant Epitopes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D011817:Rabbits; D012261:Ribonucleoproteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D012550:Schistosoma mansoni / Q000254:growth & development / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000276:immunology / Q000469:parasitology; D013601:T-Lymphocytes / Q000276:immunology*; D014466:Ultraviolet Rays; D014612:Vaccines / Q000276:immunology
D000947:Antigens, Helminth; D002135:Calcium-Binding Proteins; D037282:Calreticulin; D018984:Epitopes, T-Lymphocyte; D016056:Immunodominant Epitopes; D012261:Ribonucleoproteins; D014612:Vaccines