Micropropagation of Sapindus mukorossi Gaertn.
Bud break and multiple shoots were induced in apical and axillary meristems derived from one month old seedlings of S. mukorossi on Murashige and Skoog (MS) medium supplemented with benzylamino purine (BAP) 0.4 microM or 0.8 microM alone. A combination of BAP and gibberellic acid (GA3) 0.4 microM and 2.8 microM produced elongated multiple shoots from both types of explants. Excised shoots were rooted on MS medium respectively with indole-3-butyric acid (IBA) 3.4 microM or 2.4 microM. The regenerated plantlets were successfully acclimatized and transferred to soil.
Philomina
N S
NS
Department of Botany, S.V. University, Tirupati 517502, India.
Rao
J V
JV
D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D001947:Breeding / Q000379:methods*; D003470:Culture Media / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D016468:Forestry / Q000379:methods*; D005875:Gibberellins / Q000494:pharmacology*; D007211:Indoles / Q000494:pharmacology*; D007701:Kinetin; D018519:Meristem / Q000166:cytology / Q000187:drug effects; D009280:Naphthaleneacetic Acids / Q000494:pharmacology; D009924:Organ Culture Techniques / Q000379:methods*; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development; D011687:Purines / Q000494:pharmacology*; D014197:Trees / Q000502:physiology*
D003470:Culture Media; D005875:Gibberellins; D007211:Indoles; D009280:Naphthaleneacetic Acids; D011687:Purines; C014612:indolebutyric acid; C007842:gibberellic acid; D000225:Adenine; D007701:Kinetin