title: Human herpesvirus 8 open reading frame 26 and open reading frame 65 sequences from multiple myeloma patients: a shared pattern not found in Kaposi's sarcoma or primary effusion lymphoma.
abstract: Human herpesvirus 8 (HHV-8), also known as Kaposi's sarcoma-associated herpesvirus, has been implicated in the pathogenesis of Kaposi's sarcoma (KS), primary effusion lymphoma (PEL), multicentric Castleman's disease, and recently multiple myeloma (MM). DNA sequence analyses of HHV-8 suggest that multiple HHV-8 strains exist. We extracted DNA from 24 patients with MM and 3 patients with monoclonal gammopathy of undetermined significance and compared HHV-8 open reading frames (ORFs) 26 and 65 sequences with those derived from patients with KS, PEL, and two HHV-8-positive PEL cell lines KS-1 and BC-1. ORF26 sequence data suggest that MM patients are consistently carriers of HHV-8 strain subtype C3. All MM patients also consistently revealed either a single bp deletion or substitution at position 112197 in ORF65. This unique alteration is not present in patients with KS or PEL or in PEL cell lines. It occurs in the portion of ORF65 that is known to be responsible for a serological response to HHV-8.
authors: Division of Hematology and Oncology, Cedars-Sinai Medical Center Los Angeles, California 90048, USA.
authors: H J
authors: HJ
authors: Ma
authors: N N
authors: NN
authors: Sjak-Shie
authors: R A
authors: RA
authors: Vescio
authors: M
authors: M
authors: Kaminsky
authors: A
authors: A
authors: Mikail
authors: M
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authors: Pold
authors: K
authors: K
authors: Parker
authors: M
authors: M
authors: Beksac
authors: D
authors: D
authors: Belson
authors: T J
authors: TJ
authors: Moss
authors: C H
authors: CH
authors: Wu
authors: J
authors: J
authors: Zhou
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authors: Zhang
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authors: Chen
authors: J W
authors: JW
authors: Said
authors: J R
authors: JR
authors: Berenson
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D019288:Herpesvirus 8, Human / Q000235:genetics*; D006801:Humans; D008223:Lymphoma / Q000821:virology*; D008969:Molecular Sequence Data; D009101:Multiple Myeloma / Q000821:virology*; D016366:Open Reading Frames; D012514:Sarcoma, Kaposi / Q000821:virology*