title: Characterization of intestinal cnf1+ Escherichia coli from weaned pigs.
abstract: Escherichia coli isolated from 204 cases of porcine postweaning diarrhoea were tested by PCR for the genes of cytotoxic necrotic factors (CNF) and of cytolethal dystending toxin (CDT). Selected strains were also examined by PCR for the presence of papC-, sfa-, f17-, f18-, and afa-specific sequences encoding P, S, F17, F18 fimbriae and afimbrial adhesins. A 5.9% (12/204) of the strains had cnf1 gene, and two of them had cdt gene as well. Further six cdt+ strains were detected which were cnf-negative. Most of the cnf1+ strains belonged to serogroups O2, O6, O8, O54 characteristic of necrotoxic E. coli (NTEC) of humans. All the cnf1+ strains possessed the genes for P or S fimbriae or both, but were negative for F4, F17, or F18 or afimbrial adhesins. Results suggest that these enteric isolates may have entero- and/or uropathogenic significance in weaned pigs, and may have zoonotic potential for humans.
authors: Veterinary Medical Research Institute, Hungarian Academy of Sciences, Budapest.
authors: I
authors: I
authors: Tóth
authors: E
authors: E
authors: Oswald
authors: J G
authors: JG
authors: Mainil
authors: M
authors: M
authors: Awad-Masalmeh
authors: B
authors: B
authors: Nagy
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics*; D003603:Cytotoxins / Q000235:genetics*; D004926:Escherichia coli / Q000472:pathogenicity*; D029968:Escherichia coli Proteins; D007422:Intestines / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D013552:Swine
chemical_list: D001427:Bacterial Toxins; D003603:Cytotoxins; D029968:Escherichia coli Proteins; C067044:cytolethal distending toxin; C060296:cytotoxic necrotizing factor type 1