Characterization of glucose transport system in keratinocytes: insulin and IGF-1 differentially affect specific transporters.
Skin is one of the major tissues displaying chronic diabetic complications. We have studied glucose transport following stimulation with insulin and IGF-1 in cultured mouse keratinocytes. In proliferating cells, acute stimulation with insulin and IGF-1 increased glucose uptake. Insulin translocated glucose transporters 1 and 5, whereas IGF-1 translocated glucose transporters 2 and 3. With differentiation, glucose transporter 3 expression increased and the expression of glucose transporters 1, 2, and 5 decreased. No increase in glucose uptake was observed, however, following stimulation with either hormone. These results indicate that insulin and IGF-1 differentially regulate glucose uptake as well as expression and translocation of specific transporters in skin keratinocytes.
Shen
S
S
Faculty of Life Sciences, Gonda-Goldschmeid Center, Bar-Ilan University, Ramat-Gan, Israel.
Wertheimer
E
E
Sampson
S R
SR
Tennenbaum
T
T
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D007328:Insulin / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D015603:Keratinocytes / Q000737:chemistry* / Q000166:cytology; D051379:Mice; D009004:Monosaccharide Transport Proteins / Q000032:analysis* / Q000187:drug effects
D007328:Insulin; D009004:Monosaccharide Transport Proteins; D007334:Insulin-Like Growth Factor I; D002118:Calcium