Urine protein electrophoresis of renal transplant recipients using room temperature precipitation technique.
Proteinuria is one of the markers of renal diseases. Its quantitative measurements alone limited diagnostic conclusions. To determine the extent of precipitated proteinuria as a tool for diagnosis, I used the precipitated urine protein without any effect of any chemical and heat for protein precipitation utilizing an approach which was developed previously (Ahmed Mukhtar, 1997 & 1998). Two samples of urine (5 c.c each) from two different occasions were collected from 8 renal transplant recipients with and 7 normal healthy persons without any history of renal involvement. The samples were dried at room temperature without adding any chemical for 24 hours and precipitated urine was diluted in 0.3-0.5 ml distilled water. Then were run on Bekman apparatus Paragon Electrophoresis System and scanned for electrophoresis bands for the following proteins in BDF hospital: albumin, alpha 1, alpha 2, beta, and gamma. The pattern of electrophoresis graph and bands were different in the urine of patients compared to the urine of healthy persons. There were alterations in alpha 1 and beta proteins in the urine of renal transplant recipients. This technique illustrates a feasible approach to estimate the protein alteration in renal transplant recipients, and this pilot study could be used as a tool for further studies.
Mukhtar
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College of Health Sciences, Bahrain.
D011232:Chemical Precipitation; D004587:Electrophoresis, Agar Gel / Q000379:methods; D006801:Humans; D016030:Kidney Transplantation; D011506:Proteins / Q000032:analysis*; D011507:Proteinuria / Q000175:diagnosis*; D013696:Temperature; D014556:Urine / Q000737:chemistry*
D011506:Proteins