Adjuvant action of Escherichia coli enterotoxin for delayed-type hypersensitivity to Oka vaccine virus on pernasal co-administration in mice.
The usefulness of a mutant of Escherichia coli enterotoxin for the induction of cellular immunity to varicella-zoster virus as a mucosal adjuvant is assessed in mice. When a commercially available live varicella vaccine (the Oka strain) and toxin were once administered simultaneously via the nasal route, delayed-type hypersensitivity to Oka vaccine virus was significantly induced and detected by footpad test in mice. Moreover, when spleen cells from mice immunized with the vaccine and toxin were re-stimulated with live vaccine in vitro, they showed more thymidine uptake and produced more IL-2 than those from mice immunized with the vaccine alone. These results suggest that mutant enterotoxin has adjuvant action to induce a specific delayed-type hypersensitivity to Oka vaccine virus on nasal co-administration with live vaccine virus.
Sasaki
K
K
Department of Microbiology, Fujita Health University, School of Medicine, Toyoake, 470-1192, Aichi, Japan.
Asano
Y
Y
Honma
Y
Y
Kamiya
N
N
Handa
T
T
Ichinose
Y
Y
Yokochi
T
T
Shiraki
K
K
Tsuji
T
T
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000235:genetics; D000281:Administration, Intranasal; D000818:Animals; D001427:Bacterial Toxins / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology*; D019433:Chickenpox Vaccine / Q000008:administration & dosage*; D004768:Enterotoxins / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology*; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology*; D029968:Escherichia coli Proteins; D005260:Female; D014645:Herpesvirus 3, Human / Q000276:immunology; D006968:Hypersensitivity, Delayed; D018928:Immunity, Mucosal; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D009154:Mutation; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology
D000276:Adjuvants, Immunologic; D001427:Bacterial Toxins; D019433:Chickenpox Vaccine; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C027538:heat-labile enterotoxin, E coli