Specific removal of anti-A and anti-B antibodies by using modified dialysis filters.
Removal of anti-A and anti-B blood group antibodies from human blood has been shown to facilitate cross-matched kidney transplantation by preventing hyperacute rejection. Patients in these studies had anti-A and anti-B antibodies removed by using plasmapheresis, followed by immunoadsorption onto packed bead columns. We conducted a study to assess the feasibility of selectively removing anti-A and anti-B antibodies directly from blood by using modified dialysis filters. An anti-A and anti-B specific antigen was covalently attached to the lumenal surfaces of hollow fibers within selected commercial dialysis modules. The filters were able to reduce the anti-A and anti-B titers of 300 ml of blood to 2 or below. A low molecular weight fraction of our antigen system was found to have no antibody binding capacity. The standard antigen was purified by removal of the low molecular weight fraction and a dialysis filter was modified by using the purified antigen. This filter displayed a six-fold higher capacity than a dialysis filter modified with the same mass of standard antigen. We conclude that selective blood group antibody removal by antigen modified dialysis filters is feasible and may be a simpler system than plasmapheresis followed by immunoadsorption.
Hout
M S
MS
Department of Bioengineering, University of Pittsburgh, Pennsylvania, USA.
LeJeune
K E
KE
Schaack
T M
TM
Bristow
D K
DK
Federspiel
W J
WJ
D000017:ABO Blood-Group System / Q000276:immunology*; D003956:Dialysis / Q000295:instrumentation / Q000379:methods*; D005374:Filtration / Q000295:instrumentation / Q000379:methods; D006801:Humans; D007163:Immunosorbent Techniques; D066298:In Vitro Techniques; D007518:Isoantibodies / Q000097:blood* / Q000302:isolation & purification*; D016030:Kidney Transplantation / Q000276:immunology; D010956:Plasmapheresis
D000017:ABO Blood-Group System; D007518:Isoantibodies