Intraluminal acid and gastric mucosal integrity: the importance of blood-borne bicarbonate.
The acid-secreting gastric mucosa resists intraluminal acid better than the nonsecreting. Here we investigated pH at the epithelial cell surface, mucosal permeability, and blood flow during intraluminal administration of acid (100 mM) in acid-stimulated and nonstimulated gastric corpus mucosae. Surface pH (H(+)-selective microelectrodes), permeability (clearance of (51)Cr-EDTA), and mucosal blood flow (laser-Doppler flowmetry) were studied in Inactin-anesthetized rats. Acid secretion was stimulated with pentagastrin (40 microg. kg(-1). h(-1)) or impromidine (500 microg. kg(-1). h(-1)), or HCO(3)(-) (5 mmol. kg(-1). h(-1)) given intravenously. Surface pH was only slightly reduced by intraluminal acid in acid secretion-stimulated or HCO(3)(-)-treated rats but was substantially lowered in nonstimulated rats. Clearance increased threefold and blood flow increased by approximately 75% in nonstimulated rats. During stimulated acid secretion or intravenous infusion of HCO(3)(-), clearance was unchanged and blood flow increased by only approximately 30% during intraluminal acid. Increased epithelial transport of HCO(3)(-) buffering the mucus gel is most probably the explanation for the acid-secreting mucosa being less vulnerable to intraluminal acid than the nonsecreting.
Synnerstad
I
I
Department of Physiology, Biomedical Center, Uppsala University, S-751 23 Uppsala, Sweden.
Johansson
M
M
Nylander
O
O
Holm
L
L
D000136:Acid-Base Equilibrium / Q000502:physiology*; D000818:Animals; D002199:Capillary Permeability / Q000502:physiology; D002614:Chelating Agents / Q000493:pharmacokinetics; D002860:Chromium Radioisotopes; D004492:Edetic Acid / Q000493:pharmacokinetics; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D005744:Gastric Acid / Q000378:metabolism*; D005753:Gastric Mucosa / Q000098:blood supply* / Q000187:drug effects / Q000378:metabolism*; D017442:Histamine Agonists / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D017446:Impromidine / Q000494:pharmacology; D017078:Laser-Doppler Flowmetry; D008297:Male; D010418:Pentagastrin / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012039:Regional Blood Flow / Q000502:physiology; D017693:Sodium Bicarbonate / Q000097:blood*
D002614:Chelating Agents; D002860:Chromium Radioisotopes; D017442:Histamine Agonists; D017693:Sodium Bicarbonate; D017446:Impromidine; D004492:Edetic Acid; D010418:Pentagastrin