title: Disappearance of desmosomal components in rat corneal epithelium during wound healing.
abstract: OBJECTIVE
  Epithelial migration is essential for healing of the ablated corneal epithelium. To reveal the mechanism which enables the corneal epithelial cells to dissociate during migration, we investigated the immunolocalization of the components of the desmosome, which is the main constituent of the intercellular junction attaching the intermediate filaments to the cell surface, desmoplakin 1, desmoglein and plakoglobin, in the corneal epithelium during wound healing in rats.
  
  
  METHODS
  Under general anesthesia with ether inhalation, Wistar rats (n = 48) underwent removal of the central corneal epithelium of one eye with a small trephine and a scalpel. After various intervals of healing (5 min; 1, 3, 6, 9, 12, 24, 48 h; 1 and 2 weeks), the animals were killed and the affected eye was excised. Cryosections of each anterior segment of the eye were fixed with cold acetone and treated with primary antibodies against desmoplakin 1, desmoglein and plakoglobin. Immunolocalization of these substances was visualized by the peroxidase-diaminobenzidine reaction.
  
  
  RESULTS
  A marked immunoreactivity for these desmosome components was detected at the intercellular junction in the normal corneal and conjunctival epithelium. At 6-24 h after epithelial ablation, a weak immunoreactivity for desmoplakin 1 and plakoglobin was observed in the migrating epithelium. At 48 h after epithelial ablation, a marked immunoreactivity for these desmosome components was seen again. At 6-48 h after epithelial ablation, a weak immunoreactivity for desmoglein was observed in the migrating epithelium. At 1 week after epithelial ablation, a marked immunoreactivity for this desmosome component reappeared. The regenerated epithelium gradually exhibited normal immunolocalization of the proteins.
  
  
  CONCLUSIONS
  The desmosome components were considered to be degraded or destroyed prior to epithelial migration and reconstructed during healing of the squamous epithelium.
authors: Department of Ophthalmology, Wakayama Medical College, Wakayama, Japan.
authors: Y
authors: Y
authors: Okada
authors: S
authors: S
authors: Saika
authors: K
authors: K
authors: Shirai
authors: N
authors: N
authors: Hashizume
authors: O
authors: O
authors: Yamanaka
authors: Y
authors: Y
authors: Ohnishi
authors: E
authors: E
authors: Senba
mesh_terms: D000818:Animals; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D051182:Desmogleins; D051180:Desmoplakins; D003896:Desmosomes / Q000378:metabolism*; D019573:Epithelium, Corneal / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing; D051185:gamma Catenin
chemical_list: D003598:Cytoskeletal Proteins; D051182:Desmogleins; D051180:Desmoplakins; D051185:gamma Catenin