title: Vaccination with calpain induces a Th1-biased protective immune response against Schistosoma japonicum.
abstract: A large subunit of calpain, a calcium-activated neutral proteinase, from Schistosoma japonicum was cloned and expressed in Escherichia coli. When BALB/c mice were immunized with purified recombinant calpain (r-calpain) emulsified in complete Freund's adjuvant, a significant reduction in the number of recovered worms and also in egg production per female worm was observed (P<0.01). Spleen cells of the immunized mice showed enhanced production of gamma interferon (IFN-gamma) by activated CD4(+) T cells. Considering our observation of elevated expression of inducible nitric oxide synthase mRNA in immunized mice, r-calpain-induced IFN-gamma seemed to upregulate the production of nitric oxide by macrophages and subsequently mediated the killing of schistosomulae in the lung. On the other hand, spleen cells of immunized mice showed only faint interleukin-4 production in response to r-calpain in vitro, suggesting that immunization with r-calpain alters the Th1-Th2 balance in murine hosts even during a Th2-promoting S. japonicum infection. Furthermore, histopathological study of the livers of immunized mice showed that granulomas formed around eggs were diminished in both size and number. Egg production by female worms was clearly decreased in immunized mice, suggesting that r-calpain also has antifecundity effects. Taken together, these results point to S. japonicum calpain as a potential vaccine candidate for both worm killing and disease prevention, possibly through the induction of a strong Th1-dominant environment in immunized mice.
authors: Department of Medical Zoology, Nagoya City University Medical School, Nagoya, Japan.
authors: R
authors: R
authors: Zhang
authors: A
authors: A
authors: Yoshida
authors: T
authors: T
authors: Kumagai
authors: H
authors: H
authors: Kawaguchi
authors: H
authors: H
authors: Maruyama
authors: T
authors: T
authors: Suzuki
authors: M
authors: M
authors: Itoh
authors: M
authors: M
authors: El-Malky
authors: N
authors: N
authors: Ohta
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis; D002154:Calpain / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D006099:Granuloma / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D019001:Nitric Oxide Synthase / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D011994:Recombinant Proteins / Q000276:immunology; D012549:Schistosoma japonicum / Q000276:immunology*; D018417:Th1 Cells / Q000276:immunology*; D014611:Vaccination
chemical_list: D000909:Antibodies, Helminth; D016207:Cytokines; D012333:RNA, Messenger; D011994:Recombinant Proteins; D019001:Nitric Oxide Synthase; D002154:Calpain