title: Identification of Icm protein complexes that play distinct roles in the biogenesis of an organelle permissive for Legionella pneumophila intracellular growth.
abstract: Legionella pneumophila is a bacterial pathogen that can enter the human lung and grow inside alveolar macrophages. To grow within phagocytic host cells, the bacteria must create a specialized organelle that restricts fusion with lysosomes. Biogenesis of this replicative organelle is controlled by 24 dot and icm genes, which encode a type IV-related transport apparatus. To understand how this transporter functions, isogenic L. pneumophila dot and icm mutants were characterized, and three distinct phenotypic categories were identified. Our data show that, in addition to genes that encode the core Dot/Icm transport apparatus, subsets of genes are required for pore formation and modulation of phagosome trafficking. To understand activities required for virulence at a molecular level, we investigated protein-protein interactions. Specific interactions between different Icm proteins were detected by yeast two-hybrid and gel overlay analysis. These data support a model in which the IcmQ-IcmR complex regulates the formation of a translocation channel that delivers proteins into host cells, and the IcmS-IcmW complex is required for export of virulence determinants that modulate phagosome trafficking.
authors: Yale University School of Medicine, Section of Microbial Pathogenesis, Boyer Center for Molecular Medicine, Room 354, 295 Congress Avenue, New Haven, CT 06536, USA.
authors: J
authors: J
authors: Coers
authors: J C
authors: JC
authors: Kagan
authors: M
authors: M
authors: Matthews
authors: H
authors: H
authors: Nagai
authors: D M
authors: DM
authors: Zuckman
authors: C R
authors: CR
authors: Roy
mesh_terms: D001426:Bacterial Proteins / Q000502:physiology*; D002352:Carrier Proteins / Q000502:physiology*; D006801:Humans; D016952:Legionella pneumophila / Q000502:physiology* / Q000648:ultrastructure; D007877:Legionnaires' Disease / Q000382:microbiology* / Q000473:pathology; D008247:Lysosomes / Q000382:microbiology / Q000648:ultrastructure; D016676:Macrophages, Alveolar / Q000382:microbiology* / Q000648:ultrastructure; D015388:Organelles / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins