Reusing nylon membranes for radioactive hybridizations.
We describe a procedure for recycling nylon hybridization membranes, enabling their repeated use for radioactive Southern hybridization analysis of different DNA samples. Following hybridization and probe removal, nylon membranes containing covalently linked DNAs were treated with 0.55% sodium hypochlorite. This destroyed the DNA, thereby preventing it from participating in further hybridization and enabling the membranes to be used subsequently for binding new DNA samples. With this procedure, we were able to reuse a single membrane as many as 13 times, with no detectable loss in signal. This method was shown to be effective for membranes supplied by three different manufacturers.
Thornbury
D W
DW
University of Kentucky, Lexington, KY 40546-0091, USA.
Farman
M L
ML
D003360:Cosmids / Q000235:genetics / Q000378:metabolism; D003432:Cross-Linking Reagents; D015342:DNA Probes / Q000235:genetics / Q000378:metabolism; D004271:DNA, Fungal / Q000235:genetics / Q000378:metabolism; D020082:Magnaporthe / Q000235:genetics; D008567:Membranes, Artificial; D009691:Nucleic Acid Denaturation / Q000187:drug effects; D009693:Nucleic Acid Hybridization / Q000379:methods* / Q000528:radiation effects; D009757:Nylons / Q000528:radiation effects; D010761:Phosphorus Radioisotopes / Q000378:metabolism; D012973:Sodium Hypochlorite / Q000494:pharmacology; D014466:Ultraviolet Rays
D003432:Cross-Linking Reagents; D015342:DNA Probes; D004271:DNA, Fungal; D008567:Membranes, Artificial; D009757:Nylons; D010761:Phosphorus Radioisotopes; D012973:Sodium Hypochlorite