Fluorometric analysis of pectenotoxin-2 in microalgal samples by high performance liquid chromatography.
A rapid HPLC method with fluorescence detection of pectenotoxin-2 (PTX2), a polyether macrolide toxin, in microalgae is presented. A dienophile reagent, DMEQ-TAD, was used for precolumn fluorescence labeling. PTX2 could be quantitatively detected in the range 1-200 ng. This method confirmed the occurrence of PTX2 in net haul samples mostly composed of dinoflagellates Dinophysisspp. collected in the Adriatic Sea, Italy and Mutsu Bay, Japan.
Sasaki
K
K
Department of Applied Bioorganic Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Takizawa
A
A
Tubaro
A
A
Sidari
L
L
Loggia
R D
RD
Yasumoto
T
T
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004141:Dinoflagellida / Q000737:chemistry*; D005663:Furans / Q000032:analysis*; D018942:Macrolides; D011714:Pyrans / Q000032:analysis*; D000095702:Polyether Toxins
D005663:Furans; D018942:Macrolides; D011714:Pyrans; C098380:pectenotoxin 2; D000095702:Polyether Toxins