title: Bi-directional activation between human airway smooth muscle cells and T lymphocytes: role in induction of altered airway responsiveness.
abstract: Because both T lymphocyte and airway smooth muscle (ASM) cell activation are events fundamentally implicated in the pathobiology of asthma, this study tested the hypothesis that cooperative intercellular signaling between activated T cells and ASM cells mediates proasthmatic changes in ASM responsiveness. Contrasting the lack of effect of resting human T cells, anti-CD3-activated T cells were found to adhere to the surface of naive human ASM cells, increase ASM CD25 cell surface expression, and induce increased constrictor responsiveness to acetylcholine and impaired relaxation responsiveness to isoproterenol in isolated rabbit ASM tissues. Comparably, exposure of resting T cells to ASM cells prestimulated with IgE immune complexes reciprocally elicited T cell adhesion to ASM cells and up-regulated T cell expression of CD25. Extended studies demonstrated that: 1) ASM cells express mRNAs and proteins for the cell adhesion molecules (CAMs)/costimulatory molecules, CD40, CD40L, CD80, CD86, ICAM-1 (CD54), and LFA-1 (CD11a/CD18); 2) apart from LFA-1, ASM cell surface expression of the latter molecules is up-regulated in the presence of activated T cells; and 3) pretreatment of ASM cells and tissues with mAbs directed either against CD11a or the combination of CD40 and CD86 completely abrogated both the activated T cell-induced changes in expression of the above CAMs/costimulatory molecules in ASM cells and altered ASM tissue responsiveness. Collectively, these observations identify the presence of bi-directional cross-talk between activated T cells and ASM cells that involves coligation of specific CAMs/costimulatory molecules, and this cooperative intercellular signaling mediates the induction of proasthmatic-like changes in ASM responsiveness.
authors: Division of Pulmonary Medicine, The Joseph Stokes Jr. Research Institute, The Children's Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA. hakonarson@email.chop.edu
authors: H
authors: H
authors: Hakonarson
authors: C
authors: C
authors: Kim
authors: R
authors: R
authors: Whelan
authors: D
authors: D
authors: Campbell
authors: M M
authors: MM
authors: Grunstein
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000378:metabolism; D002450:Cell Communication / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D008297:Male; D009119:Muscle Contraction / Q000276:immunology*; D009126:Muscle Relaxation / Q000276:immunology; D009130:Muscle, Smooth / Q000166:cytology / Q000276:immunology* / Q000378:metabolism / Q000503:physiopathology*; D011817:Rabbits; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D014132:Trachea / Q000166:cytology / Q000276:immunology* / Q000378:metabolism / Q000503:physiopathology*
chemical_list: D015815:Cell Adhesion Molecules