Preparation and characterization of a new bioinorganic composite: collagen and hydroxyapatite.
Studies on biomineralization in bone have opened new arenas in the field of fabrication and engineering of bone-graft devices. A new bioinorganic composite was prepared by introducing processed collagenous matrices into the calcifying solutions at pH 7.8-8.0. The calcifying solutions were prepared by using a saturated dicalcium salt solution. Hydroxyapatite grew on the matrix over a period of 3-5 days in a specially designed crystallizing chamber. Changes in biochemical characters and biophysical parameters have given interesting insights into factors affecting and effecting initiation and progression of calcification on organic matrices.
Mythili
J
J
Biochemistry Laboratory, Central Leather Research Institute, Adyar, Chennai 600 020, India. mythly@lycos.com
Sastry
T P
TP
Subramanian
M
M
D000818:Animals; D001857:Bone Matrix / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure*; D016025:Bone Transplantation / Q000379:methods*; D002113:Calcification, Physiologic / Q000502:physiology; D002118:Calcium / Q000378:metabolism; D002152:Calorimetry, Differential Scanning; D055598:Chemical Phenomena; D015393:Chemistry, Bioinorganic; D003094:Collagen / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D003460:Crystallization; D017886:Durapatite / Q000737:chemistry / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D012996:Solutions; D013055:Spectrophotometry, Infrared; D017550:Spectroscopy, Fourier Transform Infrared
D012996:Solutions; D003094:Collagen; D017886:Durapatite; D002118:Calcium