Chemical stimulation of the dorsomedial hypothalamus elevates plasma ACTH in conscious rats.
The hallmark neuroendocrine response to stress is increased plasma ACTH. Inhibition of neurons in the region of the dorsomedial hypothalamus (DMH) attenuates experimental air stress-induced elevation of heart rate (HR), mean arterial pressure (MAP), and plasma ACTH. We hypothesized that, under basal conditions, stimulation of the DMH would mimic the neuroendocrine and cardiovascular response to air stress. We examined the effects of unilateral microinjection (100-nl vol) of bicuculline methiodide (BMI, 10 pmol), kainate (KA, 1 or 3 pmol), and N-methyl-D-aspartate (5 pmol) into the DMH or the paraventicular nucleus (PVN) on HR, MAP, locomotor activity, and plasma ACTH in conscious rats. Chemical stimulation of the DMH with KA or BMI produced increased locomotor activity and effects on HR, MAP, and plasma ACTH that together mimicked the pattern seen in experimental stress. Similar treatment in the PVN produced only small increases in MAP. Thus activation of neurons in the region of the DMH results in increased secretion of ACTH along with other changes typically seen in experimental stress.
Bailey
T W
TW
Department of Pharmacology and Toxicology, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA.
Dimicco
J A
JA
D000324:Adrenocorticotropic Hormone / Q000097:blood*; D000392:Air Movements; D000818:Animals; D001640:Bicuculline / Q000031:analogs & derivatives* / Q000494:pharmacology*; D001794:Blood Pressure; D003243:Consciousness; D004302:Dorsomedial Hypothalamic Nucleus / Q000187:drug effects* / Q000378:metabolism*; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D018756:GABA Antagonists / Q000494:pharmacology*; D006339:Heart Rate; D007030:Hypothalamo-Hypophyseal System / Q000378:metabolism; D007608:Kainic Acid / Q000494:pharmacology; D008124:Locomotion / Q000187:drug effects / Q000502:physiology; D008297:Male; D008845:Microinjections; D016202:N-Methylaspartate / Q000494:pharmacology; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism; D010913:Pituitary-Adrenal System / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013268:Stimulation, Chemical; D013312:Stress, Physiological / Q000378:metabolism
D018690:Excitatory Amino Acid Agonists; D018756:GABA Antagonists; C017069:bicuculline methiodide; D016202:N-Methylaspartate; D000324:Adrenocorticotropic Hormone; D007608:Kainic Acid; D001640:Bicuculline