The pyrroloquinoline quinone synthesis genes of Gluconobacter oxydans.
A Tn5-induced glucose dehydrogenase (GDH) deficient mutant of Gluconobacter oxydans IFO 3293 was characterised. DNA sequencing showed that the insertion site occurred in an open reading frame with homology to the pqqE gene. It was shown that acid production could be restored by addition of the coenzyme pyrroloquinoline quinone (PQQ) to the medium. The pqq cluster of G. oxydans ATCC 9937 was cloned and sequenced. It has five genes pqqA-E. The cluster could complement the Tn5-induced mutation in IFO 3293. Pulsed-field gel electrophoresis suggested that the pqq genes are not closely linked to the ribF gene that produces the riboflavin cofactor for the gluconic acid dehydrogenase.
Felder
M
M
LB Genetik, Universität Kaiserlautern, Postfach 3049, Germany.
Gupta
A
A
Verma
V
V
Kumar
A
A
Qazi
G N
GN
Cullum
J
J
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D020600:Gluconobacter oxydans / Q000235:genetics* / Q000378:metabolism; D005948:Glucose Dehydrogenases / Q000235:genetics; D005810:Multigene Family; D016254:Mutagenesis, Insertional; D045542:PQQ Cofactor; D015363:Quinolones / Q000378:metabolism*; D011809:Quinones / Q000378:metabolism*; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D015363:Quinolones; D011809:Quinones; D045542:PQQ Cofactor; D005948:Glucose Dehydrogenases