Carrier-mediated transport of riboflavin in the rat colon.
Carriers involved in riboflavin transport have generally been presumed to be localized in the upper small intestine. However, using a closed loop technique, we found that in the rat colon the absorption of riboflavin could be significantly reduced by raising the concentration from 0.1 to 200 microM and by adding lumiflavin, an analogue of riboflavin. These results suggest that saturable transport by the carrier that is specific for riboflavin and analogues may also be involved in riboflavin absorption in the colon. At the lower concentration of 0.1 microM, carrier-mediated transport was suggested to prevail, compared with passive transport, both in the colon and the small intestine. Furthermore, carrier-mediated transport in the colon was comparable with that in the small intestine. This study is the first to suggest carrier-mediated riboflavin transport in the colon. Although the riboflavin transport system in the colon needs to be subjected to more detailed investigation of its transport functions and role in riboflavin absorption after oral administration, it would be of interest to explore potential use of this carrier as a system for drug delivery.
Yuasa
H
H
Faculty of Pharmaceutical Sciences, Nagoya City University, Japan. yuasa@phar.nagoya-cu.ac.jp
Hirobe
M
M
Tomei
S
S
Watanabe
J
J
D000818:Animals; D019540:Area Under Curve; D001692:Biological Transport / Q000187:drug effects; D003106:Colon / Q000187:drug effects* / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers; D005415:Flavins / Q000494:pharmacology*; D007408:Intestinal Absorption / Q000187:drug effects; D007421:Intestine, Small / Q000187:drug effects / Q000378:metabolism; D008297:Male; D017319:Photosensitizing Agents / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D012256:Riboflavin / Q000493:pharmacokinetics*
D004337:Drug Carriers; D005415:Flavins; D017319:Photosensitizing Agents; C001922:lumiflavin; D012256:Riboflavin