title: Preimplantation genetic diagnosis for medium-chain acyl-CoA dehydrogenase (MCAD) deficiency.
abstract: Medium-chain acyl-CoA dehydrogenase (MCAD) deficiency is the most common defect in fatty acid oxidation. The disease is inherited in an autosomal recessive fashion (carrier frequency around 1 in 70) and probably affects as many as 1 in 10000 new-borns. Affected children usually present within the two first years of life with recurrent episodes of hypoketotic hypoglycaemia and lethargy leading to death in approximately 25% of the cases. One mutation (c985A-->G) accounts for approximately 90% of the carrier chromosomes. We developed a preimplantation genetic diagnosis (PGD) strategy for MCAD for a couple who had already lost two affected children. When tested on heterozygous lymphoblasts, the amplification efficiency was 67 out of 71 (94%) and the allele drop-out rate was 0 out of 67. The patient became pregnant after one PGD cycle during which two embryos were replaced. The twin pregnancy was checked by chorionic villus sampling (CVS) and was shown to be unaffected. The twins have been born and are healthy.
authors: Centre for Medical Genetics, and Centre for Reproductive Medicine, University Hospital and Medical School of the Dutch-speaking Brussels Free University (Vrije Universiteit Brussel), Laarbeeklaan 101, 1090 Brussels, Belgium. lgensnk@az.vub.ac.be
authors: K
authors: K
authors: Sermon
authors: P
authors: P
authors: Henderix
authors: W
authors: W
authors: Lissens
authors: A
authors: A
authors: De Vos
authors: M
authors: M
authors: Vandervorst
authors: A
authors: A
authors: Vanderfaeillie
authors: E
authors: E
authors: Vamos
authors: A
authors: A
authors: Van Steirteghem
authors: I
authors: I
authors: Liebaers
mesh_terms: D042964:Acyl-CoA Dehydrogenase; D044944:Acyl-CoA Dehydrogenases / Q000172:deficiency* / Q000235:genetics; D000328:Adult; D002478:Cells, Cultured; D047108:Embryonic Development; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D005820:Genetic Testing; D006801:Humans; D008052:Lipid Metabolism, Inborn Errors / Q000175:diagnosis* / Q000196:embryology / Q000201:enzymology / Q000235:genetics; D008297:Male; D017354:Point Mutation; D011247:Pregnancy; D011296:Prenatal Diagnosis
chemical_list: D005227:Fatty Acids; D044944:Acyl-CoA Dehydrogenases; D042964:Acyl-CoA Dehydrogenase