title: Healing of canine articular cartilage defects treated with microfracture, a type-II collagen matrix, or cultured autologous chondrocytes.
abstract: The effects of three different treatments on the healing of articular cartilage defects were compared with use of a previously developed canine model. In the articular surface of the trochlear grooves of 12 adult mongrel dogs, two 4-mm-diameter defects were made to the depth of the tidemark. Four dogs were assigned to each treatment group: (a) microfracture treatment, (b) microfracture with a type-II collagen matrix placed in the defect, and (c) type-II matrix seeded with cultured autologous chondrocytes. After 15 weeks, the defects were studied histologically. Data quantified on histological cross sections included areal or linear percentages of specific tissue types filling the defect, integration of reparative tissue with the calcified and the adjacent cartilage, and integrity of the subchondral plate. Total defect filling (i.e., the percentage of the cross-sectional area of the original defect filled with any type of reparative tissue) averaged 56-86%, with the greatest amount found in the dogs in the microfracture group implanted with a type-II collagen matrix. The profiles of tissue types for the dogs in each treatment group were similar: the tissue filling the defect was predominantly fibrocartilage, with the balance being fibrous tissue. There were no significant differences in the percentages of the various tissue types among dogs in the three groups.
authors: Department of Orthopaedic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors: H A
authors: HA
authors: Breinan
authors: S D
authors: SD
authors: Martin
authors: H P
authors: HP
authors: Hsu
authors: M
authors: M
authors: Spector
mesh_terms: D000818:Animals; D021461:Animals, Outbred Strains; D001672:Biocompatible Materials; D002358:Cartilage, Articular / Q000293:injuries / Q000473:pathology / Q000601:surgery*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000637:transplantation*; D003094:Collagen / Q000627:therapeutic use*; D004285:Dogs; D005109:Extracellular Matrix; D006025:Glycosaminoglycans / Q000627:therapeutic use*; D008866:Microsurgery / Q000379:methods; D019637:Orthopedic Procedures / Q000379:methods*; D014945:Wound Healing
chemical_list: D001672:Biocompatible Materials; D006025:Glycosaminoglycans; C058506:collagen-glycosaminoglycan copolymer; D003094:Collagen