title: Inactivation of the srtA gene in Streptococcus gordonii inhibits cell wall anchoring of surface proteins and decreases in vitro and in vivo adhesion.
abstract: The srtA gene product, SrtA, has been shown to be required for cell wall anchoring of protein A as well as virulence in the pathogenic bacterium Staphylococcus aureus. There are five major mechanisms for displaying proteins at the surface of gram-positive bacteria (P. Cossart and R. Jonquieres, Proc. Natl. Acad. Sci. USA 97:5013-5015, 2000). However, since many of the known surface proteins of gram-positive bacteria are believed to be exported and anchored via the sortase pathway, it was of interest to determine if srtA plays a similar role in other gram-positive bacteria. To that end, the srtA gene in the human oral commensal organism Streptococcus gordonii was insertionally inactivated. The srtA mutant S. gordonii exhibited a marked reduction in quantity of a specific anchored surface protein. Furthermore, the srtA mutant had reduced binding to immobilized human fibronectin and had a decreased ability to colonize the oral mucosa of mice. Taken together, these results suggest that the activity of SrtA plays an important role in the biology of nonpathogenic as well as pathogenic gram-positive cocci.
authors: Siga Research Laboratories, Corvallis, Oregon 97333, USA.
authors: T C
authors: TC
authors: Bolken
authors: C A
authors: CA
authors: Franke
authors: K F
authors: KF
authors: Jones
authors: G O
authors: GO
authors: Zeller
authors: C H
authors: CH
authors: Jones
authors: E K
authors: EK
authors: Dutton
authors: D E
authors: DE
authors: Hruby
mesh_terms: D019881:Aminoacyltransferases / Q000235:genetics / Q000502:physiology*; D000818:Animals; D000942:Antigens, Bacterial; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000032:analysis*; D002352:Carrier Proteins / Q000032:analysis*; D002473:Cell Wall / Q000737:chemistry; D003546:Cysteine Endopeptidases; D005353:Fibronectins / Q000502:physiology; D005798:Genes, Bacterial; D051379:Mice; D009061:Mouth Mucosa / Q000382:microbiology; D013291:Streptococcus / Q000235:genetics / Q000502:physiology*
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D005353:Fibronectins; C014689:streptococcal M protein; D019881:Aminoacyltransferases; C120542:sortase A; D003546:Cysteine Endopeptidases