Analytical considerations for microdialysis sampling.
Adaptations in microdialysis probe designs have made it possible to obtain samples from the extracellular fluid of a variety of tissues with high temporal resolution. The resulting small volume samples, often with low concentration of the analyte(s) of interest, present a particular challenge to the analytical system. Rapid separations can be coupled on-line with microdialysis to provide near real-time data. By combining microdialysis sampling with a liquid chromatographic or capillary electrophoretic separation and a highly sensitive detection method, a separation-based sensor can be developed. Such sensors have been applied to the investigation of drug entities as well as to study endogenous analytes.
Davies
M I
MI
Bioanalytical Systems, Inc., Kansas Research Laboratory, Lawrence, KS, USA.
Cooper
J D
JD
Desmond
S S
SS
Lunte
C E
CE
Lunte
S M
SM
D000818:Animals; D015374:Biosensing Techniques; D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary; D006801:Humans; D013058:Mass Spectrometry; D017551:Microdialysis