Phosphorylation of guanosine using guanosine-inosine kinase from Exiguobacterium acetylicum coupled with ATP regeneration.
Guanosine 5'-monophosphate (5'-GMP) and inosine 5'-monophosphate (5'-IMP) are widely used as flavor enhancers. Recently, a novel process for 5'-IMP production by phosphorylation of inosine using guanosine-inosine kinase coupled with ATP regeneration was reported. In this study, we demonstrated the practical possibility of producing 5'-GMP by phosphorylation of guanosine using a guanosine-inosine kinase from Exiguobacterium acetylicum coupled with ATP regeneration.
Kawasaki
H
H
Applied Microbiology Laboratory, Fermentation and Biotechnology Laboratories, Ajinomoto Co. Inc., Kawasaki-shi, Japan. hisashi_kawasaki@ajinomoto.com
Usuda
Y
Y
Shimaoka
M
M
Utagawa
T
T
D000255:Adenosine Triphosphate / Q000378:metabolism*; D001407:Bacillus / Q000201:enzymology*; D002384:Catalysis; D006151:Guanosine / Q000378:metabolism*; D010766:Phosphorylation; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism*; D013210:Staphylococcus / Q000201:enzymology*
D006151:Guanosine; D000255:Adenosine Triphosphate; D017853:Phosphotransferases (Alcohol Group Acceptor); C062896:inosine kinase