title: A comparison of human immunodeficiency virus type-1 protease inhibition activities by the aqueous and methanol extracts of Chinese medicinal herbs.
abstract: The aqueous and methanol extracts of thirty-one herbs traditionally used as anti-fever remedies in China were screened for their in vitro inhibition on human immunodeficiency virus type-1 protease (HIV-1 PR). The activity of recombinant HIV-1 protease was determined by sequence-specific cleavage at the Tyr-Pro bond of the fluorogenic substrate (Arg-Glu(EDANS)-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-Lys(DABCYL)- Arg) or by HPLC anaylsis of the cleavage products after incubation of the enzyme with a synthetic peptide substrate (Acetyl-Ser-Gln-Asn-Tyr-Pro-Val-Val-amide). Among the herbal extracts examined, the aqueous extracts of Prunella vulgaris and Scutellaria baicalensis and the methanol extracts of Woodwardia unigemmata, Paeonica suffruticosa and Spatholobus suberectus elicited significant inhibition (>90%) at a concentration of 200 microg/ml.
authors: Department of Biochemistry, The Chinese University of Hong Kong, Shatin, New Territories.
authors: T L
authors: TL
authors: Lam
authors: M L
authors: ML
authors: Lam
authors: T K
authors: TK
authors: Au
authors: D T
authors: DT
authors: Ip
authors: T B
authors: TB
authors: Ng
authors: W P
authors: WP
authors: Fong
authors: D C
authors: DC
authors: Wan
mesh_terms: D016333:HIV Protease / Q000378:metabolism*; D017320:HIV Protease Inhibitors / Q000494:pharmacology*; D015497:HIV-1 / Q000201:enzymology; D006801:Humans; D007700:Kinetics; D019684:Magnoliopsida; D008516:Medicine, Chinese Traditional; D000432:Methanol; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors; D014867:Water
chemical_list: D017320:HIV Protease Inhibitors; D010936:Plant Extracts; D011994:Recombinant Proteins; D014867:Water; D016333:HIV Protease; D000432:Methanol