title: Phytotoxicity of selected trichothecenes using Chlamydomonas reinhardtii as a model systemt.
abstract: Trichothecenes are potent inhibitors of cytoplasmic protein synthesis which can affect the severity of plant diseases such as wheat head scab. While many trichothecene-producing fungi share the initial biosynthetic intermediates, Fusarium sp. are unique in the production of trichothecenes containing an oxygen function at C-3. Although the initial trichothecene and the final products have a C-3 hydroxyl group, the intermediate steps are acetylated at C-3. By using Chlamydomonas reinhardtii, a unicellular plant with a well-defined genetic system, we were able to test the proposal that trichothecenes with a C-3 hydroxyl are more toxic to plants, as well as demonstrate that C. reinhardtii is a promising plant trichothecene bioassay system. Seven pairs of trichothecenes with either a C-3 hydroxyl or C-3 acetyl group were assayed. Our results confirm that trichothecenes acetylated at C-3 were far less toxic to Chlamydomonas than those with a C-3 hydroxyl group.
authors: Mycotoxin Research Unit, USDA/ARS, National Center for Agricutural Utilization Research, Peoria, Illinois 61604, USA. alexannj@mail.ncaur.usda.gov
authors: N J
authors: NJ
authors: Alexander
authors: S P
authors: SP
authors: McCormick
authors: S L
authors: SL
authors: Ziegenhorn
mesh_terms: D000818:Animals; D001681:Biological Assay; D016825:Chlamydomonas reinhardtii / Q000187:drug effects* / Q000254:growth & development; D005670:Fusarium / Q000378:metabolism*; D021281:Quantitative Structure-Activity Relationship; D014255:Trichothecenes / Q000737:chemistry / Q000633:toxicity*
chemical_list: D014255:Trichothecenes