Phage display in pharmaceutical biotechnology.
Over the past year, methods for the construction of M13 phage-display libraries have been significantly improved and new display formats have been developed. Phage-displayed peptide libraries have been used to isolate specific ligands for numerous protein targets. New phage antibody libraries have further expanded the practical applications of the technology and phage cDNA libraries have proven useful in defining natural binding interactions. In addition, phage-display methods have been developed for the rapid determination of binding energetics at protein-protein interfaces.
Sidhu
S S
SS
Department of Protein Engineering, Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA. sidhu@gene.com
D017104:Bacteriophage M13 / Q000235:genetics; D001435:Bacteriophages / Q000235:genetics*; D001709:Biotechnology; D015723:Gene Library; D005822:Genetic Vectors / Q000235:genetics; D019151:Peptide Library; D013678:Technology, Pharmaceutical / Q000379:methods*
D019151:Peptide Library