Nitrosomethylurea induced streptomycin resistance in Lycopersicon esculentum Mill.
High frequency of streptomycin resistant variants of Lycopersicon esculentum were isolated on selective shoot regeneration medium supplemented with IAA (0.5 mg/L), zeatin (1.5 mg/L) and streptomycin sulphate (500 mg/L). Nonmutagenized (controls) and NMU treated cotyledons were placed on shoot regeneration medium supplemented with antibiotic streptomycin. Resistant shoots appeared at a high frequency in mutagenized cotyledons, whereas in controls morphogenesis was suppressed, accompanied by bleaching. Shoot regeneration occurred from the nodular tissues developed at the cut ends of cotyledons. Resistant shoots developed into complete plantlets on rooting medium containing selective concentration of antibiotic. Stability of streptomycin resistance was confirmed by leaf assay and reciprocal crosses between streptomycin-resistant and sensitive plants.
Rao
A V
AV
Plant Biotechnology Research Unit, Department of Botany, Kakatiya University, Warangal 506009, India.
JayaSree
T
T
Ramesh
M
M
Pavan
U
U
Sadanandam
A
A
D001947:Breeding; D003433:Crosses, Genetic; D003470:Culture Media; D004351:Drug Resistance / Q000235:genetics*; D007210:Indoleacetic Acids / Q000494:pharmacology; D018551:Solanum lycopersicum / Q000187:drug effects* / Q000196:embryology / Q000235:genetics; D008770:Methylnitrosourea / Q000494:pharmacology*; D009024:Morphogenesis / Q000187:drug effects; D016296:Mutagenesis; D009153:Mutagens / Q000494:pharmacology*; D009924:Organ Culture Techniques; D018520:Plant Shoots / Q000187:drug effects; D018087:Plastids / Q000187:drug effects; D018749:RNA, Plant / Q000037:antagonists & inhibitors / Q000235:genetics; D012335:RNA, Ribosomal / Q000037:antagonists & inhibitors / Q000235:genetics; D012639:Seeds / Q000187:drug effects; D012641:Selection, Genetic; D013307:Streptomycin / Q000494:pharmacology*; D015026:Zeatin / Q000494:pharmacology
D003470:Culture Media; D007210:Indoleacetic Acids; D009153:Mutagens; D018749:RNA, Plant; D012335:RNA, Ribosomal; D008770:Methylnitrosourea; C030737:indoleacetic acid; D015026:Zeatin; D013307:Streptomycin