title: Synthesis of a statistically exhaustive fluorescent peptide substrate library for profiling protease specificity.
abstract: A statistically exhaustive, 8800 compound tripeptidal amidomethylcoumarin library was synthesized as discreet compounds using solid-phase combinatorial chemistry. A subset of the compounds was purified by HPLC and tested in a high-throughput fluorometric assay against several known serine and cysteine proteases to demonstrate the utility of this library for profiling protease substrate specificity.
authors: ChemRx Advanced Technologies, South San Francisco, CA 94080, USA. jim_sheppeck@axystech.com
authors: J E
authors: JE
authors: Sheppeck
authors: H
authors: H
authors: Kar
authors: L
authors: L
authors: Gosink
authors: J B
authors: JB
authors: Wheatley
authors: E
authors: E
authors: Gjerstad
authors: S M
authors: SM
authors: Loftus
authors: A R
authors: AR
authors: Zubiria
authors: J W
authors: JW
authors: Janc
mesh_terms: D002851:Chromatography, High Pressure Liquid; D020650:Combinatorial Chemistry Techniques; D003374:Coumarins / Q000138:chemical synthesis* / Q000737:chemistry; D010450:Endopeptidases / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D015335:Molecular Probes; D013379:Substrate Specificity
chemical_list: D003374:Coumarins; D005456:Fluorescent Dyes; D015335:Molecular Probes; C030123:coumarin; D010450:Endopeptidases