New lanostanoids from Ganoderma lucidum that induce NAD(P)H:quinone oxidoreductase in cultured hepalcic7 murine hepatoma cells.
Two new lanostanoids were isolated from the basidiocarp of Ganoderma lucidum and were identified as 26,27-dihydroxy-5 alpha-lanosta-7,9(11),24-triene-3,22-dione (1) and 26-hydroxy-5 alpha-lanosta-7,9(11),24-triene-3,22-dione (2) by their respective spectral data. Crude extracts and the isolated compounds were tested for their potential to induce NAD(P)H:quinone oxidoreductase (QR), a phase 2 drug-metabolizing enzyme, as an approach to detect potential cancer chemopreventive activity. Compound 2 doubled the specific activity of QR at a concentration of 3.0 micrograms/ml, whereas compound 1 was significantly less active (1.7-fold induction at 20 micrograms/ml). In addition, both compounds weakly inhibited sheep vesicle cyclooxygenase 1 activity at a test concentration of 40 micrograms/ml.
Ha
T B
TB
Gerhäuser
C
C
Zhang
W D
WD
Ho-Chong-Line
N
N
Fourasté
I
I
D000818:Animals; D004790:Enzyme Induction; D007810:Lanosterol / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008114:Liver Neoplasms, Experimental / Q000201:enzymology*; D051379:Mice; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000096:biosynthesis*; D020072:Polyporales / Q000737:chemistry*; D014407:Tumor Cells, Cultured
C418729:26,27-dihydroxy-5 alpha-lanosta-7,9(11),24-triene-3,22-dione; C418730:26-hydroxy-5 alpha-lanosta-7,9(11),24-triene-3,22-dione; D007810:Lanosterol; D016660:NAD(P)H Dehydrogenase (Quinone)