Removal of estrogenic activities of bisphenol A and nonylphenol by oxidative enzymes from lignin-degrading basidiomycetes.
Bisphenol A (BPA) and nonylphenol (NP) were treated with manganese peroxidase (MnP) and laccase prepared from the culture of lignin-degrading fungi. Laccase in the presence of 1-hydroxybenzotriazole (HBT), the so-called laccase-mediator system, was also applied to remove the estrogenic activity. Both chemicals disappeared in the reaction mixture within a 1-h treatment with MnP but the estrogenic activities of BPA and NP still remained 40% and 60% in the reaction mixtures after a 1-h and a 3-h treatment, respectively. Extension of the treatment time to 12 h completed the removal of estrogenic activities of BPA and NP. The laccase has less ability to remove these activities than MnP, but the laccase-HBT system was able to remove the activities in 6 h. A gel permeation chromatography (GPC) analysis revealed that main reaction products of BPA and NP may be oligomers formed by the action of enzymes. Enzymatic treatments extended to 48 h did not regenerate the estrogenic activities, suggesting that the ligninolytic enzymes are effective for the removal of the estrogenic activities of BPA and NP.
Tsutsumi
Y
Y
Department of Forest Resources Science, Shizuoka University, Ohya, Japan. afytutu@agr.shizuoka.ac.jp
Haneda
T
T
Nishida
T
T
D001487:Basidiomycota / Q000201:enzymology*; D001559:Benzhydryl Compounds; D001673:Biodegradation, Environmental; D004798:Enzymes / Q000378:metabolism*; D004968:Estrogens, Non-Steroidal / Q000378:metabolism*; D042845:Laccase; D008031:Lignin / Q000378:metabolism; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D010544:Peroxidases / Q000235:genetics / Q000378:metabolism; D010636:Phenols / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D004798:Enzymes; D004968:Estrogens, Non-Steroidal; D042845:Laccase; D008031:Lignin; D010088:Oxidoreductases; D010544:Peroxidases; D010636:Phenols; C025256:nonylphenol; C051129:manganese peroxidase; C006780:bisphenol A; D000098826:Bisphenol A Compounds