Magnetic susceptibility shift selected imaging (MESSI) and localized (1)H(2)O spectroscopy in living plant tissues.
Maize root segments permeated with aqueous solutions of the paramagnetic agents GdDTPA(2-) or DyDTPA-BMA display two well-resolved NMR peaks corresponding to the signals from intracellular and extracellular (1)H(2)O, which arise from well-understood bulk magnetic susceptibility effects. This allows each component to be studied separately. Images obtained at each frequency with MESSI editing, and single- and multiple-voxel ('spectroscopic imaging') localized spectra, clearly indicate that the agents permeate into the interstitial spaces, and into the longitudinal (xylem/phloem) channels in the stele (core) of the root, confirming earlier assessments. We believe these are the first images of a multicellular tissue acquired in vivo exclusively from the intracellular water proton resonance. This method can be further exploited to study water transport in similar systems.
Zhong
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K
Department of Chemistry, SUNY Stony Brook, NY 11794-3400, USA.
Li
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X
Shachar-Hill
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Y
Picart
F
F
Wishnia
A
A
Springer
C S
CS
D001692:Biological Transport / Q000502:physiology; D008279:Magnetic Resonance Imaging / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy; D018517:Plant Roots / Q000378:metabolism; D014867:Water / Q000737:chemistry / Q000378:metabolism*; D003313:Zea mays / Q000378:metabolism*
D014867:Water