Stimulation of melanogenesis in murine melanoma cells by 2-mercapto-1-(beta-4-pyridethyl) benzimidazole (MPB).
The effects of 2-mercapto-1-(beta-4-pyridethyl) benzimidazole (MPB), one of the benzimidazole derivatives designed for a nucleic acid analogue, on melanogenesis of murine B16-F10 melanoma cell lines were investigated. MPB (40 microM) induced a striking dendricity in B16 melanoma cells within 12 h and maximal dendricity between 48 and 72 h. The stimulation of melanin synthesis was observed after only 2 days of treatment together with a dose-dependent growth inhibition. Moreover, MPB increased the activity of tyrosinase through the expression of tyrosinase mRNA without increasing the intracellular cyclic AMP content. MPB-induced melanogenesis was inhibited by novel protein kinase A inhibitors, KT-5720 and H-85. These findings indicate that MPB stimulated B16 cells to terminally differentiate and may be a useful drug in studying the regulation of melanogenesis.
Kosano
H
H
Faculty of Pharmaceutical Sciences, Teikyo University, 1091-1 Suarashi, Sagamiko-machi, Tsukui-gun, 199-0195, Kanagawa, Japan. h-kosano@pharm.teikyo-u.ac.jp
Kayanuma
T
T
Nishigori
H
H
D000818:Animals; D001562:Benzimidazoles / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D000242:Cyclic AMP / Q000032:analysis / Q000096:biosynthesis; D003712:Dendrites / Q000502:physiology; D008543:Melanins / Q000096:biosynthesis*; D008546:Melanoma, Experimental / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D051379:Mice; D015394:Molecular Structure; D014442:Monophenol Monooxygenase / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
D001562:Benzimidazoles; D008543:Melanins; D012333:RNA, Messenger; C005770:2-mercapto-1-(beta-4-pyridethyl)benzimidazole; D000242:Cyclic AMP; D014442:Monophenol Monooxygenase