title: A functional water channel protein in the pathogenic bacterium Brucella abortus.
abstract: The gene for a new bacterial aquaporin, AqpX, was cloned from the pathogenic Gram-negative bacterium Brucella abortus. The gene was mapped on the large chromosome of B. abortus. It is flanked by one upstream and two downstream copies of the Brucella repeated sequence Bru-RS. Prediction from the nucleotide sequence indicated that the protein is a member of the MIP family, which comprises channels for water and/or solute transport. Expression in Xenopus oocytes and cryoelectron microscopy of Escherichia coli cells transformed with the aqpX gene confirmed that the protein is an efficient water channel. Glycerol uptake experiments in E. coli also showed that the protein is not able to transport glycerol.
authors: Departamento de Biologı́a Molecular, Facultad de Medicina, Universidad de Cantabria, Unidad asociada al Centro de Investigaciones Biológicas, CSIC, Cardenal Herrera Oria s/n, 39011 Santander, Spain1.
authors: Marı A C
authors: MAC
authors: Rodrı Guez
authors: UPRES-A CNRS 6026, Biologie Cellulaire et Reproduction, Equipe Canaux et Récepteurs Membranaires, Université de Rennes 1, Campus de Beaulieu, 35042 Rennes cedex, Bretagne, France2.
authors: Alexandrine
authors: A
authors: Froger
authors: UPRES-A CNRS 6026, Biologie Cellulaire et Reproduction, Equipe Canaux et Récepteurs Membranaires, Université de Rennes 1, Campus de Beaulieu, 35042 Rennes cedex, Bretagne, France2.
authors: Jean-Paul
authors: JP
authors: Rolland
authors: UPRES-A CNRS 6026, Biologie Cellulaire et Reproduction, Equipe Canaux et Récepteurs Membranaires, Université de Rennes 1, Campus de Beaulieu, 35042 Rennes cedex, Bretagne, France2.
authors: Daniel
authors: D
authors: Thomas
authors: Departamento de Biologı́a Molecular, Facultad de Medicina, Universidad de Cantabria, Unidad asociada al Centro de Investigaciones Biológicas, CSIC, Cardenal Herrera Oria s/n, 39011 Santander, Spain1.
authors: Jesús
authors: J
authors: Agüero
authors: UPRES-A CNRS 6026, Biologie Cellulaire et Reproduction, Equipe Canaux et Récepteurs Membranaires, Université de Rennes 1, Campus de Beaulieu, 35042 Rennes cedex, Bretagne, France2.
authors: Christian
authors: C
authors: Delamarche
authors: Departamento de Biologı́a Molecular, Facultad de Medicina, Universidad de Cantabria, Unidad asociada al Centro de Investigaciones Biológicas, CSIC, Cardenal Herrera Oria s/n, 39011 Santander, Spain1.
authors: Juan M
authors: JM
authors: Garcı A-Lobo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D020346:Aquaporins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D002003:Brucella abortus / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D002006:Brucellosis / Q000382:microbiology / Q000662:veterinary; D002417:Cattle; D002874:Chromosome Mapping / Q000379:methods; D003001:Cloning, Molecular; D020285:Cryoelectron Microscopy; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D008969:Molecular Sequence Data; D009865:Oocytes / Q000502:physiology; D017422:Sequence Analysis, DNA; D014867:Water / Q000378:metabolism; D014981:Xenopus / Q000235:genetics / Q000502:physiology
chemical_list: D020346:Aquaporins; D014867:Water; D005990:Glycerol