title: Effect of erythrocyte aggregation on velocity profiles in venules.
abstract: A recent whole organ study in cat skeletal muscle showed that the increase in venous resistance seen at reduced arterial pressures is nearly abolished when the muscle is perfused with a nonaggregating red blood cell suspension. To explore a possible underlying mechanism, we tested the hypothesis that red blood cell aggregation alters flow patterns in vivo and leads to blunted red blood cell velocity profiles at reduced shear rates. With the use of fluorescently labeled red blood cells in tracer quantities and a video system equipped with a gated image intensifier, we obtained velocity profiles in venous microvessels (45-75 microm) of rat spinotrapezius muscle at centerline velocities between 0.3 and 14 mm/s (pseudoshear rates 3-120 s(-1)) under normal (nonaggregating) conditions and after induction of red blood cell aggregation with Dextran 500. Profiles are nearly parabolic (Poiseuille flow) over this flow rate range in the absence of aggregation. When aggregation is present, profiles are parabolic at high shear rates and become significantly blunted at pseudoshear rates of 40 s(-1) and below. These results indicate a possible mechanism for increased venous resistance at reduced flows.
authors: Department of Bioengineering University of California, San Diego, La Jolla, California 92093, USA.
authors: J J
authors: JJ
authors: Bishop
authors: P R
authors: PR
authors: Nance
authors: A S
authors: AS
authors: Popel
authors: M
authors: M
authors: Intaglietta
authors: P C
authors: PC
authors: Johnson
mesh_terms: D000465:Algorithms; D000818:Animals; D001783:Blood Flow Velocity / Q000502:physiology*; D001794:Blood Pressure / Q000502:physiology; D003911:Dextrans / Q000494:pharmacology; D004903:Erythrocyte Aggregation / Q000502:physiology*; D005456:Fluorescent Dyes; D006400:Hematocrit; D007089:Image Enhancement; D007091:Image Processing, Computer-Assisted; D008297:Male; D018715:Microscopy, Video; D018482:Muscle, Skeletal / Q000098:blood supply*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012039:Regional Blood Flow / Q000502:physiology; D014655:Vascular Resistance; D014699:Venules / Q000502:physiology; D014741:Video Recording
chemical_list: D003911:Dextrans; D005456:Fluorescent Dyes