UV/VIS spectrophotometric methods for determination of caffeine and phenylephrine hydrochloride in complex pharmaceutical preparations. Validation of the methods.
The contents of active substances were determined in a preparation TP-4 (tablets) containing paracetamol, ascorbic acid, caffeine and phenylephrine hydrochloride. For determination of caffeine and phenylephrine hydrochloride, UV/VIS spectrophotometric method was used. The VIS spectrophotometric method based on the reaction of phenylephrine with ninhydrine in sulphuric acid (1.127 kg/l). Validation of methods performed for model mixtures proved those methods were accurate, precise, repeatable and linear in the range from 50% to 150% of the amount declared in the preparation. The content of caffeine and phenylephrine hydrochloride in TP-4, Thompyrin, Panadol Extra, Ring N satisfies the FP V demands.
Muszalska
I
I
Department of Pharmaceutical Chemistry, K. Marcinkowski University of Medical Science, 6 Grunwaldzka Str., 60-780 Poznań, Poland.
Zajac
M
M
Wróbel
G
G
Nogowska
M
M
D002110:Caffeine / Q000032:analysis*; D007202:Indicators and Reagents; D010656:Phenylephrine / Q000032:analysis*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D007202:Indicators and Reagents; D010656:Phenylephrine; D002110:Caffeine