New scheme of the biosynthesis of formononetin involving 2,7,4'-trihydroxyisoflavanone but not daidzein as the methyl acceptor.
Glycyrrhiza echinata cell-free extract produced isoformononetin by the 7-O-transmethylation of daidzein from S-adenosyl-L-methionine (SAM). When the yeast microsome expressing 2-hydroxyisoflavanone synthase was mixed with the cell-free extract and incubated with liquiritigenin and SAM, formononetin emerged. Furthermore, the cell-free extract yielded formononetin on incubation with 2,7,4'-trihydroxyisoflavanone and SAM. We propose a novel pathway of formononetin biosynthesis involving 2,7,4'-trihydroxyisoflavanone as the methyl acceptor.
Akashi
T
T
Department of Applied Biological Sciences, Nihon University, Fujisawa, Kanagawa, Japan.
Sawada
Y
Y
Aoki
T
T
Ayabe
S
S
D002474:Cell-Free System; D002851:Chromatography, High Pressure Liquid; D007529:Isoflavones / Q000737:chemistry* / Q000378:metabolism*
C420227:2,7,4'-trihydroxyisoflavanone; D007529:Isoflavones; C007768:formononetin; C004742:daidzein