title: High-density fiber-optic DNA random microsphere array.
abstract: A high-density fiber-optic DNA microarray sensor was developed to monitor multiple DNA sequences in parallel. Microarrays were prepared by randomly distributing DNA probe-functionalized 3.1-microm-diameter microspheres in an array of wells etched in a 500-microm-diameter optical imaging fiber. Registration of the microspheres was performed using an optical encoding scheme and a custom-built imaging system. Hybridization was visualized using fluorescent-labeled DNA targets with a detection limit of 10 fM. Hybridization times of seconds are required for nanomolar target concentrations, and analysis is performed in minutes.
authors: Department of Chemistry, Tufts University, Medford, Massachusetts 02155, USA.
authors: J A
authors: JA
authors: Ferguson
authors: F J
authors: FJ
authors: Steemers
authors: D R
authors: DR
authors: Walt
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D005336:Fiber Optic Technology; D008863:Microspheres; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D055100:Optical Fibers; D012680:Sensitivity and Specificity
chemical_list: D015345:Oligonucleotide Probes; D004247:DNA