Isolation and expression of the ecdysteroid-inducible angiotensin-converting enzyme-related gene in wing discs of Bombyx mori.
We isolated a clone encoding a putative angiotensin-converting enzyme-related gene from the wing disc cDNA library of the silkworm, Bombyx mori (refer to as BmAcer). The predicted open reading frame encoded 648 amino acids with about 50% identities with the Drosophila melanogaster angiotensin-converting enzyme Ance and Acer. Northern analysis identified a 2.2-kilobase mRNA which was abundant in wing discs two days after the beginning of wandering. An accumulation of the transcript was observed approximately 2 h after 20-hydroxyecdysone (20E) exposure in vitro and was blocked slightly by a protein synthetic inhibitor. These data suggest that the transcription of the BmAcer gene is directly 20E-inducible.
Quan
G X
GX
Faculty of Agriculture, Utsunomiya University, 350 Mine, Utsunomiya, 321-8505, Tochigi, Japan.
Mita
K
K
Okano
K
K
Shimada
T
T
Ugajin
N
N
Xia
Z
Z
Goto
N
N
Kanke
E
E
Kawasaki
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D029721:Drosophila Proteins; D026461:Ecdysteroids; D015870:Gene Expression; D017344:Genes, Insect; D019476:Insect Proteins / Q000235:genetics*; D008666:Metalloendopeptidases / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger; D013256:Steroids / Q000378:metabolism*; D014921:Wings, Animal
D018076:DNA, Complementary; D029721:Drosophila Proteins; D026461:Ecdysteroids; D019476:Insect Proteins; D012333:RNA, Messenger; D013256:Steroids; C102762:Acer protein, Drosophila; D008666:Metalloendopeptidases