Cloning and functional expression of the rat alpha(2) subunit of soluble guanylyl cyclase.
Nitric oxide-sensitive guanylyl cyclase is a heterodimeric enzyme consisting of one alpha and one beta subunit. Here, we clone the first alpha(2) subunit ortholog and functionally express the cDNA in Sf-9 cells. Our data indicate a high degree of conservation of the primary sequence and functional activity of the rat alpha(2) subunit.
Koglin
M
M
Institut für Experimentelle und Klinische Pharmakologie, Universität Hamburg, Martinistrasse 52, D-20251 Hamburg, Germany.
Behrends
S
S
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D017124:Conserved Sequence; D006162:Guanylate Cyclase / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D007527:Isoenzymes / Q000235:genetics; D007668:Kidney / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009569:Nitric Oxide / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012995:Solubility
D007527:Isoenzymes; D009569:Nitric Oxide; D006162:Guanylate Cyclase