How Delisea pulchra furanones affect quorum sensing and swarming motility in Serratia liquefaciens MG1.
Halogenated furanones produced by the benthic marine macroalga Delisea pulchra inhibit swarming motility of Serratia liquefaciens MG1. This study demonstrates that exogenously added furanones control transcription of the quorum sensing regulated gene swrA in competition with the cognate signal molecule N:-butanoyl-L-homoserine lactone. This in turn results in reduced production of the surface-active compound serrawettin W2, which is crucial for surface translocation of the differentiated swarm cells. It is demonstrated that furanones interfere with interspecies communication during swarming of mixed cultures and that the mode of interference in quorum-sensing control and interspecies communication is not through inhibition of autoinducer synthesis.
Rasmussen
Thomas Bovbjerg
TB
Department of Microbiology, Technical University of Denmark, 2800 Lyngby, Denmark1.
Manefield
Michael
M
Centre for Marine Biofouling and Bio-Innovation, University of New South Wales, Sydney, Australia3.|School of Microbiology and Immunology, University of New South Wales, Sydney, Australia2.
Andersen
Jens Bo
JB
Department of Microbiology, Technical University of Denmark, 2800 Lyngby, Denmark1.
Eberl
Leo
L
Lehrstuhl fur Mikrobiologie, Technische Universitat Munchen, Freising Munich, D-85350, Germany4.
Anthoni
Uffe
U
Marine Chemistry Section, H. C. Ørsted Institute, University of Copenhagen, 2100 Copenhagen, Denmark5.
Christophersen
Carsten
C
Marine Chemistry Section, H. C. Ørsted Institute, University of Copenhagen, 2100 Copenhagen, Denmark5.
Steinberg
Peter
P
Centre for Marine Biofouling and Bio-Innovation, University of New South Wales, Sydney, Australia3.|School of Microbiology and Immunology, University of New South Wales, Sydney, Australia2.
Kjelleberg
Staffan
S
Centre for Marine Biofouling and Bio-Innovation, University of New South Wales, Sydney, Australia3.|School of Microbiology and Immunology, University of New South Wales, Sydney, Australia2.
Givskov
Michael
M
Department of Microbiology, Technical University of Denmark, 2800 Lyngby, Denmark1.
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000378:metabolism; D005663:Furans / Q000378:metabolism / Q000494:pharmacology*; D015964:Gene Expression Regulation, Bacterial; D008074:Lipoproteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D008163:Luminescent Measurements; D009068:Movement / Q000187:drug effects / Q000502:physiology; D010456:Peptides, Cyclic / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D000461:Rhodophyta / Q000378:metabolism*; D012705:Serratia / Q000187:drug effects* / Q000235:genetics / Q000502:physiology*; D015398:Signal Transduction / Q000187:drug effects*; D014158:Transcription, Genetic
D005663:Furans; D008074:Lipoproteins; D010456:Peptides, Cyclic; C088386:homoserine lactone; C073497:serrawettin W2; D015107:4-Butyrolactone