Effects of anti-VLA-4 monoclonal antibody treatment in murine model of allergic rhinitis.
In order to study the role of VLA-4 in allergic rhinitis, the effects of anti-mouse VLA-4 monoclonal antibody (mAb) were evaluated in a murine model. BALB/c mice were sensitized first by i.p. injections (general sensitization) and then by daily nasal dripping of antigen (local sensitization) before performing a nasal antigen challenge. The mAb was applied either before the antigen challenge (BC group), before the local sensitization (BL group) or before the general sensitization (BS group). The effects were evaluated in terms of antigen-induced early-phase nasal symptoms (sneezing), late-phase nasal eosinophilia and the serum level of antigen-specific IgE. Antigen-induced nasal eosinophilia was significantly (p = 0.009) reduced in the BL group but not in the BC group (number of eosinophils = 114 +/- 15.1, 244 +/- 52.8 and 347 +/- 50.5 in the BL, BC and control groups, respectively). The serum level of the specific IgE was also significantly (p = 0.038) reduced in the BL group but not in the BC group (optical density = 1.18 +/- 0.07, 1.28 +/- 0.13 and 1.58 +/- 0.14 in the BL, BC and control groups, respectively). The suppressive effect on sneezing was not significant in either the BL or BC groups. In the BS group, suppressive effects on antigen-induced nasal responses and the specific IgE level were not statistically significant. These findings suggest that VLA-4 plays an important role in the topical booster or priming effects during repeated nasal antigen exposures in pre-sensitized animals.
Matsui
T
T
Department of Otolaryngology, Sapporo Medical University, School of Medicine, Japan.
Asakura
K
K
Shirasaki
H
H
Sato
J
J
Himi
T
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000276:immunology / Q000494:pharmacology*; D004195:Disease Models, Animal; D004333:Drug Administration Routes; D004804:Eosinophils / Q000187:drug effects; D007073:Immunoglobulin E / Q000097:blood / Q000187:drug effects / Q000276:immunology; D007267:Injections; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012221:Rhinitis, Allergic, Perennial / Q000188:drug therapy* / Q000276:immunology; D012912:Sneezing / Q000187:drug effects; D019010:Vascular Cell Adhesion Molecule-1 / Q000187:drug effects / Q000276:immunology
D000911:Antibodies, Monoclonal; D019010:Vascular Cell Adhesion Molecule-1; D007073:Immunoglobulin E