title: Discordant amino acid profiles in monochorionic twins with twin-twin transfusion syndrome.
abstract: To test the hypothesis that discordant growth in monochorionic (MC) twins occurs at least in part due to disparity in placental amino acid transporter function, we measured plasma amino acid levels by HPLC in maternal and fetal blood samples collected at birth from gestational age matched twins with (n = 12) and without (n = 12) twin-twin transfusion syndrome (TTTS). In the donor twin, fetal plasma concentrations and feto-maternal ratios of five essential amino acids-valine (p < 0.001), leucine (p < 0.001), iso-leucine (p < 0.05), histidine (p < 0.001) and L-arginine (p < 0. 001)-were lower than the recipient and non-TTTS twin pairs. Fetal concentrations of the nonessential amino acids taurine (p < 0.001), serine (p < 0.01), glycine (p < 0.001) and tyrosine (p < 0.05) were also markedly lower in the donor than the recipient and non-TTTS twin pairs. In contrast, the fetal alanine level in the donor twin was higher than the recipient (664 +/- 64 versus 396 +/- 23 microM; p < 0.001) and the non-TTTS twin pairs (p < 0.01). No such differences between amino acid profiles in non-TTTS MC twin pairs were found. Maternal plasma amino acid levels between TTTS and non-TTTS groups were comparable. This study provides the first evidence that certain amino acids in the donor twin of chronic TTTS differ significantly from those of the co-twin while others were comparable between twin pairs. These data, therefore, argue against inter-twin transfusion as the sole cause of growth restriction of the donor twin and suggests instead that impaired placental transport of amino acids may be a likely mechanism.
authors: Academic Units of Obstetrics and Gynaecology, St. Mary's Hospital for Women and Children, University of Manchester, UK.
authors: R
authors: R
authors: Bajoria
authors: M
authors: M
authors: Hancock
authors: S
authors: S
authors: Ward
authors: S W
authors: SW
authors: D'Souza
authors: S R
authors: SR
authors: Sooranna
mesh_terms: D000596:Amino Acids / Q000097:blood*; D000601:Amino Acids, Essential / Q000378:metabolism; D001724:Birth Weight; D004200:Diseases in Twins; D005260:Female; D005317:Fetal Growth Retardation / Q000209:etiology; D005330:Fetofetal Transfusion / Q000097:blood*; D005865:Gestational Age; D006801:Humans; D008297:Male; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D014430:Twins, Monozygotic
chemical_list: D000596:Amino Acids; D000601:Amino Acids, Essential