Effects of individual dental factors on genomic DNA analysis.
The use in forensic medicine of methods pertaining to molecular biology has made it possible to identify human remains through the analysis of polymorphic profiles of human DNA. Voluntary, accidental, or natural postmortem degradation, as well as environmental conditions, influences the preservation state of the corpse, making it sometimes difficult to obtain biologic material suitable for genetic analysis (e.g., hair, soft and/or hard tissue). According to their anatomic/morphologic characteristics, dental formations are particularly resistant to external insults and are thus suitable for this kind of research. The purpose of this work, conducted on nonselected dental findings (presenting intrinsic characteristics similar to those usually found in forensic cases) that were homogeneous with regard to environmental factors, was to determine an operative protocol that will enable combination of the maximum availability of genomic DNA with the preservation of the morphologic characteristics of the tooth for classic anthropologic evaluations.
De Leo
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D
Institute of Forensic Medicine, Department of Medicine and Public Health, University of Verona, Italy.
Turrina
S
S
Marigo
M
M
D000328:Adult; D001641:Bicuspid / Q000737:chemistry; D003481:Cuspid / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D003782:Dental Pulp / Q000473:pathology; D005260:Female; D005553:Forensic Dentistry / Q000379:methods*; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D008963:Molar / Q000737:chemistry; D011110:Polymorphism, Genetic; D020080:Tandem Repeat Sequences; D014021:Tissue Preservation
D004247:DNA