Paper-based archiving of mammalian and plant samples for RNA analysis.
The ability to archive biological samples for subsequent nucleic acid analysis is essential for tissue specimens and forensic samples. FTA Card is a chemically treated filter paper designed for the collection and room temperature storage of biological samples for subsequent DNA analysis. Its usefulness for the preservation of biological samples for subsequent RNA analysis was tested. Here, we demonstrate that RNA in biological samples stored on FTA Cards is stable and can be used successfully for RT-PCR and northern blot analysis. RNA stability depends on the storage temperature and the type of biological specimen. RNA in mammalian cells stored on FTA Cards is stable for over one year at temperatures at or below -20 degrees C and for two to three months in samples stored at room temperature. For plant leaf, longer storage times (> 5 days) require temperatures at or below -70 degrees C following sample application. FTA Cards may constitute a method not only for convenient collection and storage of biological samples but also for rapid RT-PCR analysis of tissue and cell samples.
Natarajan
P
P
Life Technologies, Rockville, MD, USA.
Trinh
T
T
Mertz
L
L
Goldsborough
M
M
Fox
D K
DK
D000818:Animals; D015152:Blotting, Northern / Q000295:instrumentation / Q000379:methods; D002460:Cell Line; D006224:Cricetinae; D015925:Cryopreservation; D006367:HeLa Cells; D006801:Humans; D010209:Paper; D012313:RNA / Q000097:blood*; D018749:RNA, Plant / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018749:RNA, Plant; D012313:RNA