title: New insights into the origin, structure and role of CD52: a major component of the mammalian sperm glycocalyx.
abstract: The sperm glycocalyx represents the primary interface between the male gamete and its environment, and gamete interaction inevitably involves interaction with this structure. Thus, it has potential significance as a target for antibodies that inhibit sperm function. Still, little is known about the components and biological role of the sperm glycocalyx. Despite the apparent complexity of the sperm membrane, surface carbohydrate labelling experiments show a high selectivity suggesting that carbohydrate side chains of CD52, an unusually short, bipolar glycopeptide of epididymal origin, form major components of the sperm glycocalyx in all mammalian species investigated. Acquisition of the highly sialylated, lipid-anchored CD52 antigen is one of the few well-defined modifications that occur to the sperm membrane during epididymal passage. It would explain changes in lectin-binding patterns and also the remarkable surface charge differences occurring during epididymal transit, most probably attributable to its terminal sialic acid residues. CD52 seems to be immunodominant on human spermatozoa, and antibodies directed against it can agglutinate and completely immobilize human sperm in the presence of complement. Expression of the same peptide backbone in lymphocytes had largely discounted its consideration as a candidate for contraceptive development. However, the recent proof of male-specific modifications indicates the feasibility of this approach.
authors: IHF, Institute for Hormone and Fertility Research at the University of Hamburg, Germany. kirchhoff@ihf.de
authors: C
authors: C
authors: Kirchhoff
authors: S
authors: S
authors: Schröter
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000737:chemistry* / Q000502:physiology*; D000951:Antigens, Neoplasm; D000074301:CD52 Antigen; D002240:Carbohydrate Sequence; D019276:Glycocalyx / Q000737:chemistry* / Q000502:physiology*; D006023:Glycoproteins / Q000737:chemistry* / Q000502:physiology*; D006801:Humans; D008297:Male; D008322:Mammals; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013094:Spermatozoa / Q000737:chemistry* / Q000502:physiology*
chemical_list: D015703:Antigens, CD; D000951:Antigens, Neoplasm; D000074301:CD52 Antigen; C000613862:CD52 protein, human; D006023:Glycoproteins