Evaluation of numerical analyses of RAPD and API 50 CH patterns to differentiate Lactobacillus plantarum, Lact. fermentum, Lact. rhamnosus, Lact. sake, Lact. parabuchneri, Lact. gallinarum, Lact. casei, Weissella minor and related taxa isolated from kocho and tef.
OBJECTIVE
The study was carried out to assess the agreement of API 50 CH fermentation data of food lactobacilli with their RAPD profiles to determine whether the system could be used alone as a reliable taxonomic tool for this genus.


RESULTS
API 50 CH, RAPD and DNA:DNA reassociation data for 42 lactobacilli from tef and kocho were compared with 30 type strains. Discrepancies were observed between the three methods in assigning strains of Lactobacillus plantarum, Lact. fermentum, Weissella minor and Lact. gallinarum, and Lact. fermentum, Lact. amylophilus, Lact. casei subsp. pseudoplantarum and Lact. rhamnosus. DNA reassociation data agreed well with RAPD results.


CONCLUSIONS
API 50 CH profiles should be complemented with molecular genetic results for effective identification in Lactobacillus.


CONCLUSIONS
The study suggested less dependability of metabolic data alone as an identification tool.
Nigatu
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Ethiopian Health and Nutrition Research Institute, Addis Ababa, Ethiopia.
D015373:Bacterial Typing Techniques; D050260:Carbohydrate Metabolism; D004269:DNA, Bacterial / Q000235:genetics; D005002:Ethiopia; D005285:Fermentation; D005516:Food Microbiology; D007777:Lactobacillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D007780:Lacticaseibacillus casei / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D006109:Poaceae / Q000382:microbiology*; D019105:Random Amplified Polymorphic DNA Technique; D019862:Zingiberales / Q000382:microbiology*
D004269:DNA, Bacterial