GTP cyclohydrolase I from Tetrahymena pyriformis: cloning of cDNA and expression.
A full-length cDNA clone for GTP cyclohydrolase I (EC 3.5.4.16) was isolated from a Tetrahymena pyriformis cDNA library by plaque hybridization. The nucleotide sequence determination revealed that the length of the cDNA insert was 1516 bp. The coding region encoded a protein of 223 amino acid residues with a calculated molecular mass of 25 416 Da. The deduced amino acid sequence of Tetrahrymena GTP cyclohydrolase I showed sequence identity with that of Escherichia coli (55%). The identity of T. pyriformis GTP cyclohydrolase I with sequences of Dictyostelium discoideum, Saccharomyces cerevisiae, Drosophila melanogaster, mouse, rat, and human enzymes was less marked and was 30, 30, 25, 28, 28, and 27%, respectively. RNA blot analysis showed a single mRNA species of 2.1 kb in this protozoan. The mRNA level of GTP cyclohydrolase I increased during synchronous cell division induced by intermittent heat treatment. The results suggest that the mRNA expression is associated with the cell cycle of T. pyriformis.
Tazawa
M
M
Department of Pharmacology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
Ohtsuki
M
M
Sumi-Ichinose
C
C
Shiraishi
H
H
Kuroda
R
R
Hagino
Y
Y
Nakashima
S
S
Nozawa
Y
Y
Ichinose
H
H
Nagatsu
T
T
Nomura
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002455:Cell Division; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D006136:GTP Cyclohydrolase / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D015723:Gene Library; D006358:Hot Temperature; D006801:Humans; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013769:Tetrahymena pyriformis / Q000201:enzymology* / Q000235:genetics*; D013997:Time Factors
D018076:DNA, Complementary; D012333:RNA, Messenger; D012313:RNA; D006136:GTP Cyclohydrolase