Tc1, from Tityus cambridgei, is the first member of a new subfamily of scorpion toxin that blocks K(+)-channels.
A new peptide, Tc1, containing only 23 amino acids closely packed by three disulfide bridges was isolated from the Amazonian scorpion Tityus cambridgei. It blocks reversibly the Shaker B K(+)-channels with a K(d) of 65 nM and displaces binding of noxiustoxin to mouse brain synaptosome membranes. It is the shortest known peptide from scorpion venom that recognizes K(+)-channels and constitutes a new structural subfamily of toxin, classified as alphaKTx 13.1.
Batista
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Department of Molecular Recognition and Structural Biology, Institute of Biotechnology, National Autonomous University of Mexico, Cuernavaca, Mexico.
Gómez-Lagunas
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Lucas
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Possani
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D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D051379:Mice; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D010455:Peptides / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D026902:Potassium Channel Blockers; D015221:Potassium Channels / Q000235:genetics; D012604:Scorpion Venoms / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D012605:Scorpions / Q000378:metabolism*; D051637:Shaker Superfamily of Potassium Channels; D018411:Spodoptera / Q000166:cytology; D013574:Synaptosomes / Q000378:metabolism
D009498:Neurotoxins; D010455:Peptides; D026902:Potassium Channel Blockers; D015221:Potassium Channels; D012604:Scorpion Venoms; D051637:Shaker Superfamily of Potassium Channels; C003609:tityustoxin