Phellinsin A, a novel chitin synthases inhibitor produced by Phellinus sp. PL3.
Phellinsin A, a novel chitin synthases inhibitor was isolated from the cultured broth of fungus PL3, which was identified as Phellinus sp. PL3. Phellinsin A was purified by solvent partition, silica gel, ODS column chromatographies, and preparative HPLC, consecutively. The structure of phellinsin A was assigned as a phenolic compound on the basis of various spectroscopic analyses including UV, IR, Mass, and NMR. Its molecular weight and formula were found to be 358 and C18H14O8, respectively. Phellinsin A selectively inhibited chitin synthase I and II of Saccharomyces cerevisiae with an IC50 value of 76 and 28 microg/ml, respectively, in our cell free assay system. This compound showed antifungal activity against Colletotrichum lagenarium, Pyricularia oryzae, Rhizoctonia solani, Aspergillus fumigatus, and Trichophyton mentagrophytes.
Hwang
E I
EI
Antibiotics Research Lab, Korea Research Institute of Bioscience & Biotechnology, Yusung, Taejon.
Yun
B S
BS
Kim
Y K
YK
Kwon
B M
BM
Kim
H G
HG
Lee
H B
HB
Jeong
W J
WJ
Kim
S U
SU
D000528:Alternaria / Q000187:drug effects; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001487:Basidiomycota / Q000737:chemistry* / Q000378:metabolism; D002175:Candida / Q000187:drug effects; D002687:Chitin Synthase / Q000037:antagonists & inhibitors*; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D007783:Lactones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010935:Plant Diseases / Q000382:microbiology
D000935:Antifungal Agents; D004791:Enzyme Inhibitors; D007783:Lactones; C417111:Phellinsin A; D010636:Phenols; D002687:Chitin Synthase