Nitric oxide-dependent neutrophil recruitment: role in nasal secretion.
Leukotriene B4 (LTB4), an inflammatory mediator, is a potent chemoattractant for neutrophils that plays an important role in nasal secretion via release of elastase. Nitric oxide (NO) is an important modulator of leucocyte-endothelial cell interactions, endogenously produced in large quantities in the paranasal sinuses. To examine the role of NO in LTB4-stimulated nasal secretion. A newly-developed method for isolating and superfusing a nasal segment in dogs was used. Instillation of LTB4 into the nasal segment caused a time-dependent increase in the volume of airway fluid and in the recruitment of neutrophils. N(G)-nitro-L-arginine-methylester (L-NAME), an inhibitor of NO synthase, prevented LTB4-induced neutrophil recruitment and nasal secretion. These studies show that NO modulates LTB4-induced neutrophil recruitment and subsequent fluid secretion in the nose, and they suggest a therapeutic role for NO inhibitors in modulating neutrophil-dependent nasal secretion.
Cardell
L O
LO
Cardiovascular Research Institute, Departments of Medicine and Physiology, University of California San Francisco, San Francisco, CA, USA.
Agustí
C
C
Nadel
J A
JA
D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D004285:Dogs; D016166:Free Radical Scavengers / Q000494:pharmacology*; D007958:Leukocyte Count; D007975:Leukotriene B4 / Q000494:pharmacology; D009297:Nasal Mucosa / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D018375:Neutrophil Activation / Q000187:drug effects; D009504:Neutrophils / Q000276:immunology*; D009569:Nitric Oxide / Q000494:pharmacology*
D016166:Free Radical Scavengers; D007975:Leukotriene B4; C026512:arginine methyl ester; D009569:Nitric Oxide; D001120:Arginine