Group A streptococcal rofA gene is involved in the control of several virulence genes and eukaryotic cell attachment and internalization.
The serotype M6 group A streptococcal RofA regulator was previously shown to exert a direct positive control of protein F1 expression and, concomitantly, fibronectin binding. Using a serotype M6 rofA mutant, we demonstrate here that this regulator has a potentially indirect negative influence on the expression of the mga, emm6, pel-sagA, and speA virulence genes. Additionally, the rofA mutant exhibited reduced eukaryotic cell internalization rates in combination with decreased host cell viability.
Beckert
S
S
Department of Medical Microbiology, University Hospital Ulm, D-89081 Ulm, Germany.
Kreikemeyer
B
B
Podbielski
A
A
D001422:Bacterial Adhesion; D001426:Bacterial Proteins; D005353:Fibronectins / Q000378:metabolism; D009154:Mutation; D013297:Streptococcus pyogenes / Q000235:genetics* / Q000472:pathogenicity; D015534:Trans-Activators / Q000235:genetics*; D014774:Virulence / Q000235:genetics
D001426:Bacterial Proteins; D005353:Fibronectins; D015534:Trans-Activators; C087448:rofA protein, Streptococcus