Reduced production of lactate during hypoxia and reoxygenation in astrocytes isolated from stroke-prone spontaneously hypertensive rats.
Lactate production and expressions of monocarboxylate transporter 1 (MCT1) and lactate dehydrogenase (LDH) mRNA after hypoxia and reoxygenation (H/R) were examined by quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) using astrocytes in culture isolated from stroke-prone spontaneously hypertensive rats (SHRSP) and Wistar Kyoto rats (WKY). The basal production of lactate in SHRSP was the same as that observed in WKY. In contrast the lactate levels in SHRSP at 1 and 6 h of reoxygenation after hypoxia were significantly lower than those observed in WKY. In addition LDH and MCT1 mRNA expressions in SHRSP were significantly less strong compared with those in WKY during H/R. These findings indicate that decreased production and slow transport of lactate in SHRSP astrocytes are involved in neuronal energy depletion and possibly encourage neuronal damage, although hereditary weakness of cortical neurons is also related to cell death during H/R.
Yamagata
K
K
Division of Life Science, Graduate School of Integrated Science and Art, University of East Asia, Shimonoseki, 751-8503, Yamaguchi, Japan. yamagata@po.cc.toua-u.ac.jp
Tagami
M
M
Ikeda
K
K
Noguchi
T
T
Yamori
Y
Y
Nara
Y
Y
D000818:Animals; D001253:Astrocytes / Q000378:metabolism* / Q000473:pathology; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D016923:Cell Death / Q000502:physiology; D002478:Cells, Cultured / Q000166:cytology / Q000378:metabolism; D005333:Fetus; D002534:Hypoxia, Brain / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D019344:Lactic Acid / Q000096:biosynthesis*; D027501:Monocarboxylic Acid Transporters; D009410:Nerve Degeneration / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR / Q000378:metabolism*; D011921:Rats, Inbred WKY / Q000378:metabolism; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D020521:Stroke / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology
D002352:Carrier Proteins; D027501:Monocarboxylic Acid Transporters; D012333:RNA, Messenger; D019344:Lactic Acid; D007770:L-Lactate Dehydrogenase