title: Plasmid-enhanced strategy for PCR-mediated mutagenesis with difficult DNA templates.
authors: University of Guelph, Guelph, ON, Canada.
authors: P D
authors: PD
authors: Abeyrathne
authors: R N
authors: RN
authors: Nazar
mesh_terms: D004271:DNA, Fungal / Q000235:genetics*; D004277:DNA, Single-Stranded / Q000235:genetics; D016297:Mutagenesis, Site-Directed / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012568:Schizosaccharomyces / Q000235:genetics; D013698:Templates, Genetic
chemical_list: D004271:DNA, Fungal; D004277:DNA, Single-Stranded