Inhalation exposure to white spirit causes region-dependent alterations in the levels of glial fibrillary acidic protein.
Enhanced expression of glial fibrillary acidic protein (GFAP) is known to be associated with toxicant-induced gliosis, a homotypic response of the central nervous system to neural injury. A variety of neurochemical and neurophysiological effects have been observed in experimental animals exposed to white spirit, but a linkage of such effects to neural damage has not been established. Here we evaluated the regional levels of GFAP to assess potential sites of CNS damage in the rat, following exposure to dearomatized and aromatic white spirit. Samples from rats exposed to dearomatized white spirit were assayed for GFAP levels in the United States and Denmark. The results were remarkably similar between countries. Small region-dependent increases and decreases in GFAP were observed with the cerebellum showing the most consistent effects (increases). In contrast, samples from rats exposed to aromatic white spirit showed large (as much as 150% of control) increases in regional levels of GFAP; again, the cerebellum showed the most consistent effects. The data are indicative of an aromatic white-spirit-induced astrogliosis in several regions of the rat CNS and suggest that chronic exposure to this solvent may be associated with underlying neural damage.
Lam
H R
HR
Institute of Food Safety and Toxicology, Danish Veterinary and Food Administration, DK-2860 Soborg, Denmark. hrl@fdir.dk
Ladefoged
O
O
Ostergaard
G
G
O'Callaghan
J P
JP
D000280:Administration, Inhalation; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D002490:Central Nervous System / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D002493:Central Nervous System Diseases / Q000139:chemically induced / Q000401:mortality; D002531:Cerebellum / Q000187:drug effects / Q000378:metabolism; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005904:Glial Fibrillary Acidic Protein / Q000187:drug effects / Q000378:metabolism; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D006838:Hydrocarbons / Q000633:toxicity*; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism; D007118:Immunoassay; D008297:Male; D008526:Medulla Oblongata / Q000187:drug effects / Q000378:metabolism; D009017:Morbidity; D009929:Organ Size / Q000187:drug effects; D011149:Pons / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D013788:Thalamus / Q000187:drug effects / Q000378:metabolism
D005904:Glial Fibrillary Acidic Protein; D006838:Hydrocarbons