title: Determination of domoic acid in Japanese mussels by enzyme immunoassay.
abstract: Ten samples of commercial blue mussels (Mytilus edulis) from Japan were analyzed for domoic acid by an indirect competitive enzyme immunoassay (idc-EIA) based on an anti-domoic acid monoclonal antibody. Domoic acid was found in all samples at low concentrations (0.11-1.81 ng/g mussel tissue). The presence of domoic acid was confirmed by liquid chromatography coupled with immunoaffinity chromatography using an anti-domoic acid monoclonal antibody as ligand. To our knowledge, this is the first reported detection of domoic acid, a causative agent of amnesic shellfish poisoning, in Japanese mussels.
authors: Osaka Prefectural Institute of Public Health, Department of Food Microbiology, Nakamichi, Japan.
authors: K
authors: K
authors: Kawatsu
authors: Y
authors: Y
authors: Hamano
authors: T
authors: T
authors: Noguchi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D049872:Bivalvia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D007124:Immunoenzyme Techniques; D007202:Indicators and Reagents; D007564:Japan; D007608:Kainic Acid / Q000031:analogs & derivatives* / Q000032:analysis*; D008387:Marine Toxins / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D007202:Indicators and Reagents; D008387:Marine Toxins; C012301:domoic acid; D007608:Kainic Acid