Protein adsorption on novel acrylamido-based polymeric ion-exchangers. I. Morphology and equilibrium adsorption.
The protein uptake equilibrium and particle morphology are determined for novel polymeric ion-exchange media based on acrylamido monomers with a high density of functional groups and a variety of morphological characteristics. The study considers two anion-exchangers and a cation-exchanger. Physical properties determined experimentally include particle density, ion-exchange capacity, particle size distribution, and equilibrium isotherms for model proteins. The pore structure was evaluated using size exclusion chromatography with neutral probe molecules and transmission electron microscopy. For the anion-exchangers, two types of structures were inferred. The first is comprised of particles that contain a low-density gel supported by denser polymer aggregates. This material had a very low size-exclusion limit for neutral probes, but exhibited an extremely high and reversible protein adsorption capacity (280-290 mg BSA/ml). The second structure is comprised of particles with large, open macropores. While the size-exclusion limit was very high, the protein adsorption capacity was low (60 mg BSA/ml). Moreover, the adsorption was nearly irreversible. The physical structure of the cation-exchanger appeared to be intermediate between those of the anion-exchangers, containing both large pores and smaller pores yielding an intermediate, but reversible, protein uptake capacity (120-130 mg alphaCHY/ml). The different behavior of these materials with regards to protein adsorption correlates well with their physical structure. For these ion-exchangers, high protein adsorption capacities are attained when a low-density polymer gel with a high concentration of functional groups is present.
Hunter
A K
AK
Department of Chemical Engineering, University of Virginia, Charlottesville 22903-2442, USA.
Carta
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D000178:Acrylamides / Q000737:chemistry*; D000327:Adsorption; D000837:Anion Exchange Resins; D002411:Cation Exchange Resins; D002850:Chromatography, Gel / Q000295:instrumentation; D002852:Chromatography, Ion Exchange / Q000295:instrumentation; D008855:Microscopy, Electron, Scanning; D011506:Proteins / Q000737:chemistry*
D000178:Acrylamides; D000837:Anion Exchange Resins; D002411:Cation Exchange Resins; D011506:Proteins