Confocal quality imaging of afferent neurons from semi-thin sections of Drosophila ganglia.
The aim of this study was to develop protocols for computer imaging of the thoraco-abdominal ganglion of Drosophila from serial semi-thin sections, in which specific neurons were stained and related to neuropilar structures. The central projections of a subset of transgenically labelled sensory neurons were revealed by immunohistochemistry, while Nomarski optics were used to show motor neuron targets in the neuropil. Digital photomicrographs of each section were aligned and the resultant image stacks rendered into three-dimensional (3D) images that can be rotated in real time. The result is a detailed, in-depth visualization of labelled neurons at a resolution comparable with that in confocal reconstructions, which also allows investigation of their relationships with other components of the neuropil.
Tyrer
N M
NM
Department of Optometry and Neuroscience, University of Manchester Institute of Science and Technology, M60 1QD, Manchester, UK.
Maddison
J
J
Shepherd
D
D
Williams
D W
DW
D000818:Animals; D030801:Animals, Genetically Modified / Q000033:anatomy & histology / Q000235:genetics; D002490:Central Nervous System / Q000166:cytology* / Q000502:physiology; D004396:Coloring Agents; D004331:Drosophila melanogaster / Q000166:cytology* / Q000235:genetics; D017952:Ganglia, Invertebrate / Q000166:cytology* / Q000502:physiology; D017930:Genes, Reporter / Q000502:physiology; D049452:Green Fluorescent Proteins; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation / Q000379:methods*; D007202:Indicators and Reagents / Q000378:metabolism; D008164:Luminescent Proteins / Q000378:metabolism; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods*; D008867:Microtomy / Q000379:methods*; D009475:Neurons, Afferent / Q000166:cytology* / Q000502:physiology
D004396:Coloring Agents; D007202:Indicators and Reagents; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins