title: Genomic and phylogenetic analysis of hepatitis C virus isolates from argentine patients: a six-year retrospective study.
abstract: Typing of hepatitis C virus (HCV) isolates from Argentine patients was performed by using different methodologies in a population of 243 patients. HCV subtype was assigned based upon restriction fragment length polymorphism (RFLP). HCV RNA genomes obtained from serum samples were classified as belonging to clade 1 (53.5%), 2 (23. 0%), or 3 (8.6%); 14.8% of samples showed HCV mixed infections, more frequently implying different subtypes within the same clade. In addition to RFLP typing, phylogenetic relatedness among sequences from both 5' untranslated region (n = 50) and nonstructural 5B coding region (n = 15) was established.
authors: Laboratorio de Hepatitis Virales, Departamento Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
authors: J F
authors: JF
authors: Quarleri
authors: B H
authors: BH
authors: Robertson
authors: V L
authors: VL
authors: Mathet
authors: M
authors: M
authors: Feld
authors: L
authors: L
authors: Espínola
authors: M P
authors: MP
authors: Requeijo
authors: O
authors: O
authors: Mandó
authors: G
authors: G
authors: Carballal
authors: J R
authors: JR
authors: Oubiña
mesh_terms: D020121:5' Untranslated Regions / Q000737:chemistry; D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D005260:Female; D005838:Genotype; D016174:Hepacivirus / Q000145:classification / Q000235:genetics*; D006801:Humans; D008099:Liver / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012189:Retrospective Studies; D015819:Substance Abuse, Intravenous / Q000150:complications
chemical_list: D020121:5' Untranslated Regions