Isolation and rapid identification of an abundant self-peptide from class II HLA-DRB1*0401 alleles induced by measles vaccine virus infection.
Class II HLA-DR genes play an important role in the immune response to viral antigens. The effect of measles vaccine virus (MVV) infection on the induction of self-peptides presented by HLA-DR molecules during the immune response to viral infection is poorly known. Here, we describe a strategy for isolation and rapid sequence determination of an MVV-inducible class II bound peptide from a membrane protein (Leu-13). Peptides bound to HLA-DR4 (DRB1*0401 peptide complex) were eluted from immunoaffinity-purified HLA-DR4, peptides were differentially screened by MALDI-TOF-MS and subsequently sequenced by post source decay (PSD)-MALDI-TOF-MS. Human B-cells infected with MVV demonstrated an enhanced pattern of self-peptide production after MVV infection. This relatively simple analytical protocol provides a sensitive method for the direct identification of peptides associated with MHC class II DR molecules. More broadly, this same approach can be used to identify sequences of specific MVV processed peptides presented by any class II MHC DR molecule.
Ovsyannikova
I G
IG
Mayo Vaccine Research Group, Clinical Pharmacology Unit, Mayo Clinic and Foundation, Rochester, MN 55905, USA.
Johnson
K L
KL
Naylor
S
S
Poland
G A
GA
D000483:Alleles; D000595:Amino Acid Sequence; D000943:Antigens, Differentiation / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D001402:B-Lymphocytes / Q000276:immunology / Q000821:virology; D002461:Cell Line, Transformed; D005786:Gene Expression Regulation; D006684:HLA-DR Antigens / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D059811:HLA-DRB1 Chains; D006801:Humans; D008458:Measles Vaccine / Q000276:immunology*; D008459:Measles virus / Q000276:immunology*; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D000943:Antigens, Differentiation; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; C553505:HLA-DRB1*04:01 antigen; D008458:Measles Vaccine; D009842:Oligopeptides; C062010:leu-13 antigen