New method for standardization of electropherograms obtained in capillary zone electrophoresis.
A new method for standardization of electropherograms obtained by capillary zone electrophoresis was proposed, where the migration time axis was replaced by the effective mobility axis. The mobility increase due to temperature increase by Joule heating and the relaxation effect of the potential gradient were eliminated successfully by introducing a temperature coefficient for mobility expression and a delay time, respectively. The precision of the mobility evaluated by the proposed conversion methods was evaluated for a model sample. By using the conversion method, almost the same electropherograms could be obtained even from the electropherograms originally obtained by using different hardware conditions.
Ikuta
N
N
Applied Physics and Chemistry, Faculty of Engineering, Hiroshima University, Higashi-Hiroshima, Japan. nakki@ipc.hiroshima-u.ac.jp
Yamada
Y
Y
Yoshiyama
T
T
Hirokawa
T
T
D019075:Electrophoresis, Capillary / Q000379:methods* / Q000592:standards*