title: Attachment of A-431 cells on immobilized antibodies to the EGF receptor promotes cell spreading and reorganization of the microfilament system.
abstract: EGF-like sequences, inherent in a number of extracellular matrix proteins, participate in cell adhesion. It is possible that interactions of these sequences with EGF receptors (EGFR) affect actin filament organization. It was shown previously [Khrebtukova et al., 1991: Exp. Cell Res. 194:48-55] that antibodies specific to EGFR induce capping of these receptors and redistribution of cytoskeletal proteins in A-431 cells. Here we report that A-431 cells attach and spread on solid substrata coated with antibodies to EGFR, even in the absence of serum. Thus, EGFR can act as an adhesion protein and promote microfilament reorganization. Binding of the cells to the EGFR-antibody resulted in the formation of a unique cell shape characterized by numerous, actin-based filopodia radiating from the cell body, but without membrane ruffles. There was also a conspicuous circular belt of actin-containing fibers inside the cell margin, and many irregular actin aggregates in the perinuclear area. The morphologies and actin distributions in A-431 cells spread on fibronectin or laminin 2/4 were very different. On fibronectin, cells had polygonal shapes with numerous stress-fibers and thick actin-containing fibers along the cell edges. On laminin-covered substrata, the cells became fusiform and acquired broad leading lamellae with ruffles. In these cells, there were also a few bundles of filaments running the whole length of the cell body, and shorter bundles extending through the leading lamellae towards the membrane ruffles in the cell edge. These effects and those seen with immobilized EGF suggest that different ligand/receptor complexes induce specific reorganizations of the microfilament system.
authors: Department of Cell Culture, Institute of Cytology, Russian Academy of Sciences, St.-Petersburg, Russia.
authors: A
authors: A
authors: Are
authors: G
authors: G
authors: Pinaev
authors: E
authors: E
authors: Burova
authors: U
authors: U
authors: Lindberg
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism* / Q000648:ultrastructure; D000199:Actins / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism*; D002413:Cations, Divalent / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology*; D002465:Cell Movement; D048429:Cell Size; D016895:Culture Media, Serum-Free; D003571:Cytochalasin B / Q000031:analogs & derivatives / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000378:metabolism; D066246:ErbB Receptors / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D008024:Ligands; D008856:Microscopy, Fluorescence; D011554:Pseudopodia / Q000648:ultrastructure; D014407:Tumor Cells, Cultured
chemical_list: D000199:Actins; D000911:Antibodies, Monoclonal; D002413:Cations, Divalent; D016895:Culture Media, Serum-Free; D008024:Ligands; C024794:dihydrocytochalasin B; D003571:Cytochalasin B; D004815:Epidermal Growth Factor; D066246:ErbB Receptors