Reestablishment of in vitro and in vivo iodide uptake by transfection of the human sodium iodide symporter (hNIS) in a hNIS defective human thyroid carcinoma cell line.
Uptake of iodide is a prerequisite for radioiodine therapy in thyroid cancer. However, loss of iodide uptake is frequently observed in metastasized thyroid cancer, which may be explained by diminished expression of the human sodium iodide symporter (hNIS). Strategies to restore iodide uptake in thyroid cancer include the exploration of hNIS gene transfer into hNIS defective thyroid cancer. In this study, we report the stable transfection of a hNIS expression vector into the hNIS defective follicular thyroid carcinoma cell line FTC133. Stablely transfected colonies exhibited high uptake of Na125I, which could be blocked completely with sodiumperchlorate. hNIS mRNA expression corresponded with iodide uptake in semiquantitative polymerase chain reaction. Iodide uptake was maximal after 60 minutes, whereas iodide efflux was complete after 120 minutes. hNIS transfected FTC133 and control cell lines injected subcutaneously in nude mice formed tumors after 6 weeks. Iodide uptake in the hNIS transfected tumor was much higher than in the nontransfected tumor, which corresponded with hNIS mRNA expression in tumors.
Smit
J W
JW
Department of Endocrinology and Metabolism, Leiden University Medical Center, The Netherlands. j.w.a.smit.endo@lumc.nl
Shröder-van der Elst
J P
JP
Karperien
M
M
Que
I
I
van der Pluijm
G
G
Goslings
B
B
Romijn
J A
JA
van der Heide
D
D
D000328:Adult; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism*; D004195:Disease Models, Animal; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes / Q000493:pharmacokinetics; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D008819:Mice, Nude; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012974:Sodium Iodide / Q000493:pharmacokinetics*; D027981:Symporters; D013964:Thyroid Neoplasms; D014162:Transfection; D014407:Tumor Cells, Cultured; D000099209:Sodium-Iodide Symporters
D002352:Carrier Proteins; D007457:Iodine Radioisotopes; D008565:Membrane Proteins; D012333:RNA, Messenger; D012974:Sodium Iodide; D027981:Symporters; D000099209:Sodium-Iodide Symporters