title: Chromatography of human prothrombin from Nitschmann fraction III on DEAE Sepharose Fast Flow using axial and radial flow column.
abstract: An axial column (3 x 2.6 cm) and a radial flow column (3.5 x 5 cm) packed with DEAE Sepharose Fast Flow media was evaluated for the separation of human prothrombin from Nitschmann fraction III. Under radial flow conditions, a sample flow rate up to 14 mL/min (approximately 18 bed vols/h) was achieved. Breakthrough capacity was determined and both columns had almost the same breakthrough capacity per mL media, indicating that the sample loading was independent of radial column geometry.
authors: Institute of Transfusion Medicine, PO Box 130(9), 27 TaiPing Road, Beijing 100850, People's Republic of China. sunt@cin.bmi.ac.cn
authors: T
authors: T
authors: Sun
authors: G
authors: G
authors: Chen
authors: Y
authors: Y
authors: Liu
authors: F
authors: F
authors: Bu
authors: M
authors: M
authors: Wen
mesh_terms: D002852:Chromatography, Ion Exchange / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D011516:Prothrombin / Q000302:isolation & purification*
chemical_list: D011516:Prothrombin