PCR-restriction enzyme analysis of a bone marrow isolate from a human immunodeficiency virus-positive patient discloses polyclonal infection with two Mycobacterium avium strains.
Polyclonal infection by Mycobacterium avium was detected by hsp65 PCR-restriction enzyme analysis (PRA) in a bone marrow isolate from an AIDS patient. Two M. avium strains, differing in colony morphology, PRA HaeIII digestion pattern, insertion element (IS) 1245 amplification, and restriction fragment length polymorphism fingerprints with IS1245 and IS1311 probes, were isolated.
Oliveira
R S
RS
Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo-Escola Paulista de Medicina, São Paulo, Brazil.
Sircili
M P
MP
Ueki
S Y
SY
Telles
M A
MA
Schnabel
B
B
Briones
M R
MR
Leão
S C
SC
D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology*; D000328:Adult; D001483:Base Sequence; D001853:Bone Marrow / Q000382:microbiology*; D004262:DNA Restriction Enzymes / Q000494:pharmacology; D004251:DNA Transposable Elements; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D015269:Mycobacterium avium Complex / Q000235:genetics* / Q000302:isolation & purification; D015270:Mycobacterium avium-intracellulare Infection / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D004251:DNA Transposable Elements; D004262:DNA Restriction Enzymes