title: Genome-wide location and function of DNA binding proteins.
abstract: Understanding how DNA binding proteins control global gene expression and chromosomal maintenance requires knowledge of the chromosomal locations at which these proteins function in vivo. We developed a microarray method that reveals the genome-wide location of DNA-bound proteins and used this method to monitor binding of gene-specific transcription activators in yeast. A combination of location and expression profiles was used to identify genes whose expression is directly controlled by Gal4 and Ste12 as cells respond to changes in carbon source and mating pheromone, respectively. The results identify pathways that are coordinately regulated by each of the two activators and reveal previously unknown functions for Gal4 and Ste12. Genome-wide location analysis will facilitate investigation of gene regulatory networks, gene function, and genome maintenance.
authors: Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA.
authors: B
authors: B
authors: Ren
authors: F
authors: F
authors: Robert
authors: J J
authors: JJ
authors: Wyrick
authors: O
authors: O
authors: Aparicio
authors: E G
authors: EG
authors: Jennings
authors: I
authors: I
authors: Simon
authors: J
authors: J
authors: Zeitlinger
authors: J
authors: J
authors: Schreiber
authors: N
authors: N
authors: Hannett
authors: E
authors: E
authors: Kanin
authors: T L
authors: TL
authors: Volkert
authors: C J
authors: CJ
authors: Wilson
authors: S P
authors: SP
authors: Bell
authors: R A
authors: RA
authors: Young
mesh_terms: D001665:Binding Sites; D002453:Cell Cycle; D004271:DNA, Fungal / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D005690:Galactose / Q000378:metabolism; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D016681:Genome, Fungal; D000072235:Mating Factor; D020411:Oligonucleotide Array Sequence Analysis; D010455:Peptides / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors / Q000378:metabolism*; D015533:Transcriptional Activation
chemical_list: D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C052130:GAL4 protein, S cerevisiae; D010455:Peptides; C489241:STE12 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D000072235:Mating Factor; D005690:Galactose