Nucleotide sequence, genome organization and phylogenetic analysis of pineapple mealybug wilt-associated virus-2.
The genome of pineapple mealybug wilt-associated closterovirus-2 (PMWaV-2) was cloned from double-stranded RNA isolated from diseased pineapple and its sequence determined. The 3'-terminal 14861 nt of the single-stranded RNA genome contains ten open reading frames (ORFs) which, from 5' to 3', potentially encode a >204 kDa polyprotein containing papain-like protease, methyltransferase and helicase domains (ORF1a), a 65 kDa RNA-dependent RNA polymerase (ORF1b), a 5 kDa hydrophobic protein (ORF2), a 59 kDa heat shock protein 70 homologue (ORF3), a 46 kDa protein (ORF4), a 34 kDa coat protein (ORF5), a 56 kDa diverged coat protein (ORF6), a 20 kDa protein (ORF7), a 22 kDa protein (ORF8) and a 6 kDa protein (ORF9). A 132 nt untranslated region was present at the 3' terminus of the genome. This genome organization is typical of the monopartite closteroviruses, including the putative +1 ribosomal frameshift allowing expression of ORF1b. Phylogenetic analysis revealed that within the family CLOSTEROVIRIDAE: the mealybug-transmitted PMWaV-2 is more closely related to other mealybug-transmitted members than to those which are transmitted by aphids or whiteflies. Within this group, PMWaV-2 shares the greatest sequence identity with grapevine leafroll-associated virus-3, another mealybug-transmitted closterovirus.
Melzer
M J
MJ
Department of Plant Pathology, University of Hawaii, Honolulu, HI 96822, USA1.
Karasev
A V
AV
Department of Microbiology and Immunology, Thomas Jefferson University, Doylestown, PA 18901, USA2.
Sether
D M
DM
Department of Plant Pathology, University of Hawaii, Honolulu, HI 96822, USA1.
Hu
J S
JS
Department of Plant Pathology, University of Hawaii, Honolulu, HI 96822, USA1.
D000818:Animals; D003001:Cloning, Molecular; D017790:Closterovirus / Q000145:classification / Q000235:genetics*; D005638:Fruit / Q000821:virology*; D016679:Genome, Viral; D007313:Insecta / Q000821:virology; D008969:Molecular Sequence Data; D016366:Open Reading Frames