Purification and characterisation of a non-plant myrosinase from the cabbage aphid Brevicoryne brassicae (L.).
Plant myrosinases and glucosinolates constitute a defence system in cruciferous plants towards pests and diseases. We have purified for the first time a non-plant myrosinase from the cabbage aphid Brevicoryne brassicae (L.) to homogeneity. The protein was N-terminally blocked and protease (trypsin and lys c) degradation gave peptides of which five were sequenced. The protein is a dimer with subunits of mass 54 kDa+/-500 Da. Western blot analysis with an anti-aphid myrosinase antibody showed a strong cross reaction with a protein extract from the Brassica specialist, B. brassicae. The anti-aphid myrosinase antibody does not cross react with plant myrosinase neither does an anti-plant myrosinase antibody cross react with aphid myrosinase.
Jones
A M
AM
Imperial College at Wye, Wye, Kent TN25 5AH, Ashford, UK.
Bridges
M
M
Bones
A M
AM
Cole
R
R
Rossiter
J T
JT
D000818:Animals; D001042:Aphids / Q000201:enzymology*; D001937:Brassica; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D008970:Molecular Weight; D011817:Rabbits
D006026:Glycoside Hydrolases; C022739:thioglucosidase