Mapping of B-cell epitopes on human parvovirus B19 non-structural and structural proteins.
In the present study, the immune reactivity to Parvovirus B19 (B19) proteins and variations in antigenic reactivity in different clinical manifestations were investigated. Sera from healthy B19 IgG positive individuals were evaluated for antibody reactivity against linear peptides. Three antigenic regions (amino acid number 191-206, 271-286, 371-386) on the B19 non-structural (NS) protein 1 were identified. The highest seroreactivity against these peptides was found against amino acid number 271-286. Seroreactivity in this group of individuals was also investigated against peptides representing selected neutralising regions of the B19 capsid proteins viral protein (VP) 1/VP2. The antigenic NS1 and VP1/VP2 regions, thus defined, were further mapped by seroreactivity against peptides containing specific deletions. The frequencies of seroreactivity against the NS1 and VP1/VP2 peptides in healthy B19 IgG positive individuals were similar to those in HIV-seropositive and persistently B19 infected patients, except that the latter group showed a lower reactivity to the C-terminal end of VP1/VP2. The identification of antigenic regions and corresponding seroreactivity in asymptomatic and persistently B19-infected patients is important for the understanding of B19-pathogenesis and for the development of B19 vaccine candidates.
Tolfvenstam
T
T
Department of Clinical Virology, F68, Karolinska Institutet, Huddinge University Hospital, SE-14186, Stockholm, Sweden. thomas.tolfvenstam@impi.ki.se
Lundqvist
A
A
Levi
M
M
Wahren
B
B
Broliden
K
K
D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000235:genetics* / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D002213:Capsid / Q000235:genetics / Q000276:immunology; D036022:Capsid Proteins; D018604:Epitope Mapping; D006801:Humans; D008969:Molecular Sequence Data; D010322:Parvoviridae Infections / Q000276:immunology; D016732:Parvovirus B19, Human / Q000235:genetics* / Q000276:immunology*; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000276:immunology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; C106202:NS1 protein, parvovirus; D017361:Viral Nonstructural Proteins; C095547:capsid protein VP1, parvovirus B19; C095548:capsid protein VP2, parvovirus B19