title: Aromatic compound-dependent Brucella suis is attenuated in both cultured cells and mouse models.
abstract: The aroC gene of the facultative intracellular pathogen Brucella suis was cloned and sequenced. The cloned aroC gene complements Escherichia coli and Salmonella enterica serovar Typhimurium aroC mutants. A B. suis aroC mutant was found to be unable to grow in a defined medium without aromatic compounds. The mutant was highly attenuated in tissue culture (THP1 macrophages and HeLa cells) and murine virulence models.
authors: INSERM U431, Faculté de Médecine, 30900 Nîmes, France.
authors: V
authors: V
authors: Foulongne
authors: K
authors: K
authors: Walravens
authors: G
authors: G
authors: Bourg
authors: M L
authors: ML
authors: Boschiroli
authors: J
authors: J
authors: Godfroid
authors: M
authors: M
authors: Ramuz
authors: D
authors: D
authors: O'Callaghan
mesh_terms: D000818:Animals; D002002:Brucella / Q000254:growth & development / Q000472:pathogenicity*; D003001:Cloning, Molecular; D003470:Culture Media; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D019761:Phosphorus-Oxygen Lyases / Q000235:genetics / Q000502:physiology*; D014774:Virulence
chemical_list: D003470:Culture Media; C019895:chorismate synthase; D019761:Phosphorus-Oxygen Lyases