title: Phosphorylation of guanosine using guanosine-inosine kinase from Exiguobacterium acetylicum coupled with ATP regeneration.
abstract: Guanosine 5'-monophosphate (5'-GMP) and inosine 5'-monophosphate (5'-IMP) are widely used as flavor enhancers. Recently, a novel process for 5'-IMP production by phosphorylation of inosine using guanosine-inosine kinase coupled with ATP regeneration was reported. In this study, we demonstrated the practical possibility of producing 5'-GMP by phosphorylation of guanosine using a guanosine-inosine kinase from Exiguobacterium acetylicum coupled with ATP regeneration.
authors: Applied Microbiology Laboratory, Fermentation and Biotechnology Laboratories, Ajinomoto Co. Inc., Kawasaki-shi, Japan. hisashi_kawasaki@ajinomoto.com
authors: H
authors: H
authors: Kawasaki
authors: Y
authors: Y
authors: Usuda
authors: M
authors: M
authors: Shimaoka
authors: T
authors: T
authors: Utagawa
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D001407:Bacillus / Q000201:enzymology*; D002384:Catalysis; D006151:Guanosine / Q000378:metabolism*; D010766:Phosphorylation; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism*; D013210:Staphylococcus / Q000201:enzymology*
chemical_list: D006151:Guanosine; D000255:Adenosine Triphosphate; D017853:Phosphotransferases (Alcohol Group Acceptor); C062896:inosine kinase