Effects of leucocyte depletion on rheologic properties of human CPDA-1 blood.
OBJECTIVE
Leucocyte depletion improves the quality of stored blood units. We have studied its role on blood viscosity.


METHODS
Viscosity of CPDA-1 blood units was measured in a Couette viscometer at shear rates of 94.5 and 0.1 s(-1) prior to and following filtration with the Leukotrap((R)) A1 system on day 0 and after 21 days at +4 degrees C.


RESULTS
On day 0, high but not low shear viscosity was significantly decreased. The red blood cell morphology was unaffected. On day 21, blood viscosity was increased similarly for unfiltered and filtered samples at both shear rates. The echinocytosis observed after storage correlated with the increase in viscosity.


CONCLUSIONS
Leucocyte depletion is associated with a decrease in high shear viscosity. This effect is, however, completely lost after 21 days.
Walter
R
R
Department of Internal Medicine, Kantonsspital, Chur, Switzerland.
Brand
B
B
Mark
M
M
Schnyder
L
L
Stifanic
M
M
Reinhart
W H
WH
D000225:Adenine / Q000494:pharmacology; D000368:Aged; D000369:Aged, 80 and over; D000925:Anticoagulants / Q000494:pharmacology; D001809:Blood Viscosity; D002951:Citrates / Q000494:pharmacology; D004912:Erythrocytes / Q000648:ultrastructure; D005260:Female; D005374:Filtration; D005947:Glucose / Q000494:pharmacology; D018056:Hemorheology; D006801:Humans; D007937:Leukapheresis; D007962:Leukocytes; D008297:Male; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D010710:Phosphates / Q000494:pharmacology; D012996:Solutions / Q000494:pharmacology
D000925:Anticoagulants; C034876:CPDA solutions; D002951:Citrates; D010710:Phosphates; D012996:Solutions; D005947:Glucose; D000225:Adenine