Damage induction by direct electric current in tumoural target cells.
Damage induction to tumour target cells (P815) by direct electric current (DC) was investigated. A 6 min treatment of P815 cells with DC generated decreased levels of cell viability and proliferation. The ultrastructural analysis of DC-treated cells revealed the presence of blebs, loss of cell surface filopodia, and ruptures in cell membrane. Mitochondrial alterations, swelling of cells, cytoplasmic matrix rarefaction, and cellular debri formation were also observed. The study shows that tumoural target cells can be damaged by direct electric current and this approach may provide means to understand the mechanism of tumour regression induced by electrochemical therapy.
Holandino
C
C
Departamento de Medicamentos-Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Veiga
V F
VF
Capella
M M
MM
Menezes
S
S
Alviano
C S
CS
D000818:Animals; D016923:Cell Death; D002455:Cell Division; D004599:Electric Stimulation Therapy; D004560:Electricity; D012515:Mast-Cell Sarcoma / Q000473:pathology*; D051379:Mice; D008854:Microscopy, Electron; D014407:Tumor Cells, Cultured / Q000648:ultrastructure