The MAK-V protein kinase regulates endocytosis in mouse.
We report the cloning of a mouse cDNA encoding the MAK-V protein kinase, with a putative specificity for serine/threonine residues. The mak-v gene is transcribed in adult brain and in the mouse embryo from at least 7.5 dpc. Using the yeast two-hybrid system, we showed that MAK-V interacts with Rabaptin-5, a protein which plays an important role in endocytosis. Functional studies of the MAK-V protein suggest that it regulates endocytosis. We also constructed a human mak-v cDNA and localized the human mak-v gene at 21q22.11. Its chromosomal location suggests that mak-v could be involved in disorders of the nervous system, development or in malignancies.
Korobko
I V
IV
Institute of Gene Biology, Russian Academy of Sciences, Moscow. igorvk@mx.ibg.relarn.ru
Korobko
E V
EV
Kiselev
S L
SL
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000201:enzymology; D002891:Chromosomes, Human, Pair 21 / Q000235:genetics; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004705:Endocytosis / Q000502:physiology*; D015870:Gene Expression; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009417:Nerve Tissue / Q000378:metabolism; D011494:Protein Kinases / Q000235:genetics* / Q000502:physiology*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000502:physiology*; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D020798:Two-Hybrid System Techniques
D017931:DNA Primers; D018076:DNA, Complementary; D011494:Protein Kinases; C417729:Hunk protein, mouse; D017346:Protein Serine-Threonine Kinases