Partial characterization of serum immunoglobulins of Oreochromis mossambicus.
Serum immunoglobulins of O. mossambicus were purified using chromatography methods--CM affinity gel blue chromatography followed by two step purification involving a combination of ion-exchange and gel filtration chromatography. Studies revealed that O. mossambicus produces only one class of high molecular weight macroglobulin as determined by molecular sieving by Sepharose CL 6-B. Immunoelectrophoresis of purified O. mossambicus serum against rabbit anti O. mossambicus serum gave only a single precipitin line. Further analysis of the immunoglobulin by SDS-PAGE showed that the IgM macroglobulin weighs about 900,000 Da, composed of mu-like heavy chain weighing about 90 kDa each and light chains weighing about 30 kDa each.
Rajavarthini
P B
PB
Centre for Fish Immunology, Postgraduate and Research Department of Zoology, American College, Madurai 625002.
Arunkumar
R I
RI
Michael
R D
RD
D000818:Animals; D001797:Blood Protein Electrophoresis; D002417:Cattle; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006386:Hemagglutination Tests; D007114:Immunization; D007122:Immunoelectrophoresis; D007075:Immunoglobulin M / Q000737:chemistry; D007136:Immunoglobulins / Q000737:chemistry / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*; D008970:Molecular Weight; D011234:Precipitins / Q000097:blood / Q000276:immunology; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000276:immunology; D017210:Tilapia / Q000097:blood / Q000276:immunology*
D007075:Immunoglobulin M; D007136:Immunoglobulins; D011234:Precipitins; D012710:Serum Albumin, Bovine