Prospective screening by a panfungal polymerase chain reaction assay in patients at risk for fungal infections: implications for the management of febrile neutropenia.
Invasive fungal infections are a major cause of mortality in neutropenic cancer patients. To determine whether a polymerase chain reaction (PCR)-based assay enabled the identification of patients at risk for invasive fungal infections, a prospective monitoring once per week was performed during 92 neutropenic episodes in patients receiving chemotherapy for acute leukaemia or high-dose therapy followed by allogeneic or autologous stem cell transplantation, with the investigators blinded to clinical and microbiological data. PCR positivity was documented in 34 out of 92 risk episodes. All patients developing proven invasive fungal infection were found PCR positive, and PCR was found to be the earliest indicator of invasive fungal infection preceding clinical evidence by a mean of 5.75 d (range 0-14 d). In febrile neutropenic patients without a prior history of invasive fungal infection, a sensitivity of 100% and a specificity of 73% of the PCR assay for the development of proven or probable invasive fungal infection was documented. In conclusion, panfungal PCR performed prospectively once a week enabled the identification of patients at high risk for invasive fungal infections.
Hebart
H
H
Department of Haematology and Oncology, University of Tübingen, Germany.
Löffler
J
J
Reitze
H
H
Engel
A
A
Schumacher
U
U
Klingebiel
T
T
Bader
P
P
Böhme
A
A
Martin
H
H
Bunjes
D
D
Kern
W V
WV
Kanz
L
L
Einsele
H
H
D000208:Acute Disease; D000328:Adult; D000368:Aged; D000666:Amphotericin B / Q000627:therapeutic use; D000935:Antifungal Agents / Q000627:therapeutic use; D001228:Aspergillosis / Q000235:genetics*; D001232:Aspergillus fumigatus / Q000235:genetics; D002177:Candidiasis / Q000235:genetics*; D005260:Female; D005335:Fever of Unknown Origin / Q000382:microbiology*; D005800:Genes, Fungal; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D017403:In Situ Hybridization; D007951:Leukemia, Myeloid / Q000276:immunology* / Q000382:microbiology / Q000628:therapy; D008107:Liver Diseases / Q000382:microbiology; D008172:Lung Diseases, Fungal / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D008991:Monitoring, Physiologic; D009503:Neutropenia / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity
D000935:Antifungal Agents; D000666:Amphotericin B