Rapid determination of ethylene glycol and glycolic acid in biological fluids.
Ethylene glycol poisoning of companion animals is a common occurrence and is sometimes involved in human intoxication. Ethylene glycol is of limited toxicity, but the metabolites including glycolic acid are responsible for poisoning. Conventional treatment has employed substances to prevent alcohol dehydrogenase from metabolizing the ethylene glycol, but to be effective, therapy must begin within hours of ethylene glycol consumption. We describe a rapid (10 min) analysis of biological fluids for ethylene glycol and glycolic acid using isocratic HPLC, a refractive index detector, and a Waters fast fruit juice analytical column.
Smith
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Livestock Disease Diagnostic Center, Department of Veterinary Science, University of Kentucky, Lexington 40511, USA.
Lang
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D000818:Animals; D002371:Cat Diseases / Q000139:chemically induced* / Q000175:diagnosis; D002415:Cats; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D004283:Dog Diseases / Q000139:chemically induced* / Q000175:diagnosis; D004285:Dogs; D019855:Ethylene Glycol / Q000032:analysis / Q000097:blood / Q000506:poisoning*; D005122:Exudates and Transudates / Q000737:chemistry; D005260:Female; D005766:Gastrointestinal Contents / Q000737:chemistry; D006016:Glycolates / Q000032:analysis / Q000097:blood / Q000506:poisoning*; D008297:Male
D006016:Glycolates; C031149:glycolic acid; D019855:Ethylene Glycol