Cell-dependent gag mutants of HIV-1 are crucially defective at the stage of uncoating/reverse transcription in non-permissive cells.
We have previously shown that some of the human immunodeficiency virus type 1 (HIV-1) gag matrix (MA), capsid (CA), and nucleocapsid (NC) mutants display host-cell-dependent replication potential, and that they are defective at the early phase of the virus replication cycle in non-permissive cells. To determine the defective replication stage of the cell-dependent mutants precisely, the processes of virus entry into cells and virus DNA synthesis were monitored by the highly sensitive enzyme-linked immunosorbent assay and polymerase chain reaction amplification analysis. The results obtained indicated that all the cell-dependent MA, CA and NC mutants are defective at the stage of uncoating/reverse transcription, and that a cellular factor(s) is involved in this process.
Koh
K
K
Department of Virology, School of Medicine, The University of Tokushima, 770-8503, Tokushima, Japan.
Miyaura
M
M
Yoshida
A
A
Sakurai
A
A
Fujita
M
M
Adachi
A
A
D002213:Capsid / Q000235:genetics / Q000502:physiology*; D036022:Capsid Proteins; D002460:Cell Line; D004279:DNA, Viral / Q000096:biosynthesis; D003673:Defective Viruses / Q000235:genetics / Q000502:physiology; D015683:Gene Products, gag / Q000235:genetics / Q000502:physiology*; D015488:HIV Antigens / Q000235:genetics / Q000502:physiology*; D016655:HIV Core Protein p24 / Q000235:genetics / Q000502:physiology*; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D009154:Mutation; D014158:Transcription, Genetic; D014162:Transfection; D014764:Viral Proteins; D014771:Virion / Q000737:chemistry; D014779:Virus Replication; D054301:gag Gene Products, Human Immunodeficiency Virus
D036022:Capsid Proteins; D004279:DNA, Viral; D015683:Gene Products, gag; D015488:HIV Antigens; D016655:HIV Core Protein p24; C071884:NCP7 protein, Human immunodeficiency virus 1; D014764:Viral Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus; C054858:p17 protein, Human Immunodeficiency Virus Type 1