title: Specification of neurotransmitter receptor identity in developing retina: the chick ATH5 promoter integrates the positive and negative effects of several bHLH proteins.
abstract: Genetic studies in Drosophila and in vertebrates have implicated basic helix-loop-helix (bHLH) transcription factors in neural determination and differentiation. In this report, we analyze the role that several bHLH proteins play in the transcriptional control of differentiation in chick retina. Our experimental system exploits the properties of the promoter for the beta 3 subunit of the neuronal acetylcholine receptors, important components of various phenotypes in the CNS of vertebrates. The beta 3 subunit contributes to define ganglion cell identity in retina and its promoter, whose activation is an early marker of ganglion cell differentiation, is under the specific control of the chick atonal homolog ATH5. Functional analysis of the ATH5 promoter indicates that interactions between ATH5 and several other bHLH transcription factors underlie the patterning of the early retinal neuroepithelium and form a regulatory cascade leading to transcription of the gene for beta 3. ATH5 appears to coordinate the transcriptional pathways that control pan-neuronal properties with those that regulate the subtype-specific features of retinal neurons.
authors: Department of Biochemistry, Sciences II, University of Geneva, Switzerland.
authors: L
authors: L
authors: Matter-Sadzinski
authors: J M
authors: JM
authors: Matter
authors: M T
authors: MT
authors: Ong
authors: J
authors: J
authors: Hernandez
authors: M
authors: M
authors: Ballivet
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D002642:Chick Embryo; D002645:Chickens; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D029721:Drosophila Proteins; D018507:Gene Expression Regulation, Developmental; D006133:Growth Substances; D018257:Helix-Loop-Helix Motifs / Q000235:genetics*; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics; D011401:Promoter Regions, Genetic; D011950:Receptors, Cholinergic / Q000235:genetics*; D012160:Retina / Q000196:embryology* / Q000378:metabolism*; D012165:Retinal Ganglion Cells / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013234:Stem Cells / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics; D029961:Zebrafish Proteins
chemical_list: C490910:ASH1 protein, Drosophila; D017931:DNA Primers; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D006133:Growth Substances; D009419:Nerve Tissue Proteins; D011950:Receptors, Cholinergic; D014157:Transcription Factors; D029961:Zebrafish Proteins; C413414:atoh7 protein, zebrafish