A convenient fluorometric method for the detection of extracellular N-acetylglucosaminidase production by filamentous fungi.
A rapid and convenient method for the detection of chitinases accumulating in filamentous fungal cultures was developed. The assay is performed on cultures growing in microtiter plates, with a fluorogenic substrate: 4-methylumbelliferyl-N-acetyl-D-glucosaminide (4-MeUNAG). The fluorescence of the product, 4-methylumbelliferone, was detected. This method was successfully used to follow induction and repression of extracellular exochitinase activity in the biocontrol fungus Trichoderma harzianum.
Omero
C
C
Otto Warburg Center for Agricultural Biotechnology, The Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot, Israel. chet@agri.huji.ac.il
Horwitz
B A
BA
Chet
I
I
D000118:Acetylglucosaminidase / Q000378:metabolism*; D003470:Culture Media; D005470:Fluorometry / Q000379:methods; D006923:Hymecromone / Q000378:metabolism; D012680:Sensitivity and Specificity; D014242:Trichoderma / Q000201:enzymology* / Q000254:growth & development*
D003470:Culture Media; D006923:Hymecromone; D000118:Acetylglucosaminidase