Enzyme-histochemical demonstration of lymphatic vessels in the golden hamster periodontium: an attempt to reactivate the 5'-nucleotidase activity with Mg++ ion supply.
Enzyme-histochemical demonstration of lymphatic vessels in the golden hamster periodontium was performed on cryostat sections using the 5'-nucleotidase (5'-Nase) staining method by light microscopy and backscattered electron imaging of scanning electron microscopy. The inhibition of the 5'-Nase activity by decalcification was cancelled by the Mg++ ion supply. The reaction products of 5'-Nase activity were produced on the lymphatic endothelial cells and the tubular structures of lymphatic vessels were seen as highlights by backscattered electron imaging. The invasion of 5'-Nase-positive lymphatic vessels into the alveolar bone from the periodontium was found in the present study.
Nara
E
E
Oral Anatomy I, Iwate Medical University School of Dentistry, Morioka, Japan. einara@iwate-med.ac.jp
Osawa
T
T
Liang
J C
JC
Wang
Y
Y
Nozaka
Y
Y
D015720:5'-Nucleotidase / Q000302:isolation & purification*; D000818:Animals; D006224:Cricetinae; D004583:Electrons; D008208:Lymphatic System / Q000033:anatomy & histology*; D008647:Mesocricetus; D008854:Microscopy, Electron / Q000379:methods*; D010519:Periodontium / Q000033:anatomy & histology*; D012542:Scattering, Radiation; D013194:Staining and Labeling / Q000379:methods*
D015720:5'-Nucleotidase