Quantification of Ca2+/H+ antiporter VCAX1p in vacuolar membranes and its absence in roots of mung bean.
Vacuolar Ca2+/H+ antiporter VCAX1p, which contributes to the Ca2+ accumulation into vacuoles, was quantified by immunochemistry. The antiporter content in vacuolar membranes was 0.14 and 1.1 microg mg(-1) of membrane protein for hypocotyls and epicotyls, respectively. The calculated turnover number was 120 s(-1). Roots lacked the antiporter protein and the transcript.
Ueoka-Nakanishi
H
H
Laboratory of Biochemistry, Graduate School of Bioagricultural Sciences, Nagoya University, Japan.
Maeshima
M
M
D017920:Antiporters / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D027682:Cation Transport Proteins; D007887:Fabaceae / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism; D018546:Hypocotyl / Q000378:metabolism; D043564:Inorganic Pyrophosphatase; D007425:Intracellular Membranes / Q000378:metabolism; D007700:Kinetics; D010946:Plants, Medicinal; D006180:Proton-Translocating ATPases / Q000378:metabolism; D011755:Pyrophosphatases / Q000378:metabolism; D012639:Seeds / Q000378:metabolism*; D025262:Vacuolar Proton-Translocating ATPases; D014617:Vacuoles / Q000378:metabolism*
D017920:Antiporters; D002135:Calcium-Binding Proteins; D027682:Cation Transport Proteins; C027614:calcium-hydrogen antiporters; D006859:Hydrogen; D011755:Pyrophosphatases; D025262:Vacuolar Proton-Translocating ATPases; D043564:Inorganic Pyrophosphatase; D006180:Proton-Translocating ATPases