title: Mitochondrial calcium overload is restricted to a few mitochondria in endothelial cells after cold ischemia/reperfusion.
abstract: Changes in cytosolic and mitochondrial calcium content were studied in an endothelial cell model after simulating cold ischemia reperfusion injury. Image analysis demonstrated that only a subpopulation of mitochondria in endothelial cells accumulate calcium. Observations led to the hypothesis that mitochondria which are in close contact with the plasma membrane are mainly affected by the Ca2+ efflux across that membrane, while those in other parts of the cell remain unaffected.
authors: Department of Transplant Surgery, University Hospital Innsbruck, Austria.
authors: A
authors: A
authors: Amberger
authors: H
authors: H
authors: Weiss
authors: T
authors: T
authors: Haller
authors: R
authors: R
authors: Margreiter
mesh_terms: D000241:Adenosine; D000493:Allopurinol; D002118:Calcium / Q000502:physiology*; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology*; D005978:Glutathione; D006801:Humans; D007328:Insulin; D007511:Ischemia; D008928:Mitochondria / Q000502:physiology*; D019188:Organ Preservation Solutions; D011887:Raffinose; D015424:Reperfusion; D014471:Umbilical Veins
chemical_list: D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose; D002118:Calcium