title: Role of cortical tumour-suppressor proteins in  asymmetric division of Drosophila neuroblast.
abstract: Cellular diversity during development arises in part from asymmetric divisions, which generate two distinct cells by transmitting localized determinants from a progenitor cell into one daughter cell. In Drosophila, neuroblasts undergo typical asymmetric divisions to produce another neuroblast and a ganglion mother cell. At mitosis, neural fate determinants, including Prospero and Numb, localize to the basal cortex, from which the ganglion mother cell buds off; Inscuteable and Bazooka, which regulate spindle orientation, localize apically. Here we show that a tumour-suppressor protein, Lethal giant larvae (Lgl), is essential for asymmetric cortical localization of all basal determinants in mitotic neuroblasts, and is therefore indispensable for neural fate decisions. Lgl, which itself is uniformly cortical, interacts with several types of Myosin to localize the determinants. Another tumour-suppressor protein, Lethal discs large (Dlg), participates in this process by regulating the localization of Lgl. The localization of the apical components is unaffected in lgl or dlg mutants. Thus, Lgl and Dlg act in a common process that differentially mediates cortical protein targeting in mitotic neuroblasts, and that creates intrinsic differences between daughter cells.
authors: CREST, Japan Science and Technology Corporation, Sendai.
authors: T
authors: T
authors: Ohshiro
authors: T
authors: T
authors: Yagami
authors: C
authors: C
authors: Zhang
authors: F
authors: F
authors: Matsuzaki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D019070:Cell Lineage; D003931:Diacetyl / Q000031:analogs & derivatives* / Q000494:pharmacology; D004330:Drosophila / Q000196:embryology / Q000235:genetics; D029721:Drosophila Proteins; D016147:Genes, Tumor Suppressor; D019476:Insect Proteins / Q000235:genetics / Q000502:physiology*; D007605:Juvenile Hormones / Q000502:physiology; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology; D008938:Mitosis; D008969:Molecular Sequence Data; D009154:Mutation; D018995:Myosin Heavy Chains / Q000235:genetics / Q000502:physiology; D009218:Myosins / Q000502:physiology; D009474:Neurons / Q000166:cytology*; D025521:Tumor Suppressor Proteins
chemical_list: D018797:Cell Cycle Proteins; D029721:Drosophila Proteins; D019476:Insect Proteins; D007605:Juvenile Hormones; D008565:Membrane Proteins; C107613:Mira protein, Drosophila; D025521:Tumor Suppressor Proteins; C097862:Zip protein, Drosophila; C081435:l(2)gl protein, Drosophila; C060606:numb protein, Drosophila; C070350:dlg1 protein, Drosophila; C004717:diacetylmonoxime; D018995:Myosin Heavy Chains; D009218:Myosins; D003931:Diacetyl