In vitro catabolism of histidine by mixed rumen bacteria and protozoa.
An in vitro study was conducted to examine the metabolism of histidine (His) by mixed rumen bacteria (B), mixed rumen protozoa (P), and a combination of the two (BP). Rumen microorganisms were collected from fistulated goats fed with lucerne cubes (Medicago sativa) and a concentrate mixture twice a day. Microbial suspensions were anaerobically incubated with or without 2 mm each of His, or histamine (HTM), or 1 mm urocanic acid (URA) at 39 degrees C for 12 h. His and other related compounds in both supernatant and microbial hydrolysates were analyzed by HPLC. After 6- and 12-h incubations, the net degradation of His was 26.1% and 51.7% in B, 13.5% and 20.9% in P, and 21.7% and 46.0% in BP, respectively. The rate of the net degradation of His in B (98.0 micromol/g microbial nitrogen/h) was about 2.6 times higher than that of P during a 12-h incubation period. His was found to be degraded into urocanic acid (URA), imidazolelactic acid (ImLA), imidazoleacetic acid (ImAA), and histamine (HTM). Of these degraded His was mainly converted into URA in all microbial suspensions. The production of ImLA and ImAA was higher in B than in P suspensions, whereas the production of HTM was higher in P than in B suspensions. From these results, the existence of diverse catabolic routes of His in rumen microorganisms was indicated.
Wadud
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Laboratory of Animal Nutrition and Biochemistry, Division of Animal Science, Miyazaki University, Miyazaki shi 889-2192, Japan. rafatkun@hotmail.com
Onodera
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Or-Rashid
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Oshiro
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D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003470:Culture Media; D056890:Eukaryota / Q000378:metabolism*; D006041:Goats / Q000382:microbiology / Q000469:parasitology; D006639:Histidine / Q000378:metabolism*; D007093:Imidazoles; D012417:Rumen / Q000382:microbiology* / Q000469:parasitology*
D003470:Culture Media; D007093:Imidazoles; D006639:Histidine