Cloning of Rac and Rho-GDI from tobacco using an heterologous two-hybrid screen.
To examine whether molecular similarities exist between the animal and plant Rho GTPase signaling pathways, we have developed a heterologous two-hybrid screening method. By this technique, we have cloned a cDNA encoding a tobacco Rac-like protein able to interact with a mammalian Rho-GDI. In a second screen this tobacco Rac was used as a bait and a tobacco homologue of Rho-GDI was identified. These results show that some components of the animal and plant Rac signaling pathways are similar enough to allow their interaction in an heterologous approach. Moreover these data suggest a similar regulation of Rho GTPases in animals and plants.
Kieffer
F
F
Laboratoire de Phytopharmacie et de Biochimie des Interactions Cellulaires, INRA, BV 1540, 21034 cedex, Dijon, France.
Elmayan
T
T
Rubier
S
S
Simon-Plas
F
F
Dagher
M C
MC
Blein
J P
JP
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D020730:Guanine Nucleotide Dissociation Inhibitors / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D010947:Plants, Toxic; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism; D020798:Two-Hybrid System Techniques; D020744:rac GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D062947:rho-Specific Guanine Nucleotide Dissociation Inhibitors
D020730:Guanine Nucleotide Dissociation Inhibitors; D010940:Plant Proteins; D062947:rho-Specific Guanine Nucleotide Dissociation Inhibitors; D020744:rac GTP-Binding Proteins