title: Recent advances in the protocols of transgenic mouse mutation assays.
abstract: Transgenic mutation assays were developed to detect gene mutations in multiple organs of mice or rats. The assays permit (1) quantitative measurements of mutation frequencies in all tissues/organs including germ cells and (2) molecular analysis of induced and spontaneous mutations by DNA sequencing analysis. The protocols of recently developed selections in the lambda phage-based transgenic mutation assays, i.e. cII, Spi(-) and 6-thioguanine selections, are described, and a data set of transgenic mutation assays, including those using Big Blue and Muta Mouse, is presented.
authors: Division of Genetics and Mutagenesis, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, 158-8501, Tokyo, Japan. nohmi@nihs.go.jp
authors: T
authors: T
authors: Nohmi
authors: T
authors: T
authors: Suzuki
authors: K
authors: K
authors: Masumura
mesh_terms: D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D016296:Mutagenesis; D009152:Mutagenicity Tests / Q000379:methods*