Apoptosis and cell proliferation capacity in AKR lymphoma malignancy variants.
Dysregulation in apoptotic cell death has recently emerged as a factor in tumorigenesis, but its effect in tumor progression is not yet established. In the present study we evaluated the levels of proliferative and apoptotic cell fractions in a T-cell lymphoma tumor progression model. We compared these features and the expression of apoptosis-related genes in primary tumors of several AKR lymphoma malignancy variants. According to DNA flow cytometry, a considerable proportion of cells (35-40%) was in the proliferative (S + G2/M) phase in all variants, but a slight augmentation with increasing malignancy was noted. Apoptotic cell content was, unexpectedly, the lowest in the less malignant variant. This might be due to the higher content in macrophages observed in this variant, which possibly partly eliminated apoptotic bodies. We found an increase in bcl-2 level with increasing malignancy that was probably counterbalanced by the simultaneous increase observed in the Fas receptor.
Donin
N
N
Department of Pathology, Sackler Faculty of Medicine, Tel-Aviv University, Israel.
Kay
S
S
Sinai
J
J
Katzenelson
D
D
Siegal
A
A
Michowitz
M
M
Schibi
G
G
Leibovici
J
J
D000818:Animals; D017209:Apoptosis; D001399:Azure Stains; D002455:Cell Division; D053938:DNA Fragmentation; D004273:DNA, Neoplasm / Q000032:analysis; D018450:Disease Progression; D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D016158:Genes, p53 / Q000235:genetics; D008223:Lymphoma / Q000737:chemistry* / Q000235:genetics / Q000473:pathology*; D051379:Mice; D008806:Mice, Inbred AKR; D009479:Neuropeptides / Q000032:analysis*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis*; D011519:Proto-Oncogenes / Q000235:genetics; D018124:Receptors, Tumor Necrosis Factor; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*; D019014:fas Receptor
D001399:Azure Stains; D004273:DNA, Neoplasm; C491899:Fas protein, mouse; D009479:Neuropeptides; D019253:Proto-Oncogene Proteins c-bcl-2; D018124:Receptors, Tumor Necrosis Factor; D016159:Tumor Suppressor Protein p53; D019014:fas Receptor