The epithelial sodium channel (ENaC) in rodent retina, ontogeny and molecular identity.
OBJECTIVE
To assess the appearance of the epithelial sodium channels (ENaC) during retinal development and establish its molecular identity.


METHODS
Photoreceptors were isolated by horizontal sectioning of rat retina with a vibratome series 1000. Intact retina and isolated photoreceptors were analyzed for the developmental appearance of ENaC using the Polymerase Chain Reaction (PCR) technique. Immunofluorescence was conducted with the aid of an antibody raised against the 14 amino acids peptide QGLGKGDKREEQGL, corresponding to the N-terminal region (44-58 aa) of alpha ENaC.


RESULTS
ENaC message was present in rat retina photoreceptors, isolated just one day after birth. The bipolar and ganglion cell layers, separated from whole retina by vibratome, also contained the ENaC message. The partial sequence of the photoreceptor ENaC (496 base) exhibited one hundred percent homology with the channel from rat known sources. Immunochemistry revealed that only the outer nuclear layer was positive for the ENaC in one-day-old rat; the inner segment became immunopositive at the age of 9 days.


CONCLUSIONS
The ENaC is present in the retina and visible soon after birth. These observations suggest that ENaC may prove to have physiological importance in the retina but until now its function is unknown.
Golestaneh
N
N
Faculté de Médecine Paris VI, INSERM E9912, Paris, France.
Nicolas
C
C
Picaud
S
S
Ferrari
P
P
Mirshahi
M
M
D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D053503:Epithelial Sodium Channels; D019084:Fluorescent Antibody Technique, Indirect; D015870:Gene Expression; D010446:Peptide Fragments; D020419:Photoreceptor Cells, Vertebrate / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D011817:Rabbits; D051381:Rats; D012160:Retina / Q000254:growth & development* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D015222:Sodium Channels / Q000096:biosynthesis* / Q000235:genetics
D017931:DNA Primers; D053503:Epithelial Sodium Channels; D010446:Peptide Fragments; D012333:RNA, Messenger; D015222:Sodium Channels