Chiral determination of amino acids by capillary electrophoresis and laser-induced fluorescence at picomolar concentrations.
In this publication we present results on the determination of enantiomers of amino acids at very low concentrations. A fluoresceine-based chiral dye was synthesized to allow the separation of diastereoisomers of D- and L-amino acids. We used capillary electrophoresis with different non-ionic surfactants (Brij). The separation parameters were optimized and separations of D- and L-isovaline, an unusual terrestrial amino acid, were obtained. The sensitivity limits were also determined using a commercial laser-induced fluorescence detector. The quantitation of these amino acids is very important to understand the process of chiral selection on Earth.
Vandenabeele-Trambouze
O
O
SCA-CNRS, Echangeur de Solaize, Vernaison, France. odile_trambouze@post.club-internet.fr
Albert
M
M
Bayle
C
C
Couderc
F
F
Commeyras
A
A
Despois
D
D
Dobrijevic
M
M
Loustalot
M F
MF
D000596:Amino Acids / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005453:Fluorescence; D007834:Lasers; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism
D000596:Amino Acids