Spectroscopic analysis of neural activity in brain: increased oxygen consumption following activation of barrel cortex.
This research investigates the hemodynamic response to stimulation of the barrel cortex in anaesthetized rats using optical imaging and spectroscopy (Bonhoeffer and Grinvald, 1996; Malonek and Grinvald, 1996; Mayhew et al., 1999). A slit spectrograph was used to collect spectral image data sequences. These were analyzed using an algorithm that corrects for the wavelength dependency in the optical path lengths produced by the light scattering properties of tissue. The analysis produced the changes in the oxy- and deoxygenation of hemoglobin following stimulation. Two methods of stimulation were used. One method mechanically vibrated a single whisker, the other electrically stimulated the whisker pad. The electrical stimulation intensity varied from 0.4 to 1.6 mA. The hemodynamic responses to stimulation increased as a function of intensity. At 0.4 mA they were commensurate with those from the mechanical stimulation; however, the responses at the higher levels were greater by a factor of approximately 10. For both methods of data collection, the results of the spectroscopic analysis showed an early increase in deoxygenated hemoglobin (Hbr) with no evidence for a corresponding decrease in oxygenated hemoglobin (HbO(2)). Evidence for increased oxygen consumption (CMRO(2)) was obtained by converting the fractional changes in blood volume (Hbt) into estimates of changes in blood flow (Grubb et al., 1974) and using the resulting time course to scale the fractional changes in Hbr. The results show an early increase CMRO(2) peaking approximately 2 s after stimulation onset. Using these methods, we find evidence for increased oxygen consumption following increased neural activity even at low levels of stimulation intensity.
Mayhew
J
J
Artificial Intelligence Vision Research Unit and Department of Psychology, University of Sheffield, Sheffield S10 2TP, United Kingdom.
Johnston
D
D
Berwick
J
J
Jones
M
M
Coffey
P
P
Zheng
Y
Y
D000465:Algorithms; D000818:Animals; D001931:Brain Mapping; D006454:Hemoglobins / Q000378:metabolism; D008465:Mechanoreceptors / Q000502:physiology*; D010101:Oxygen Consumption / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D013003:Somatosensory Cortex / Q000502:physiology*; D013057:Spectrum Analysis; D009435:Synaptic Transmission / Q000502:physiology*; D014738:Vibrissae / Q000294:innervation*
D006454:Hemoglobins; C100003:deoxyhemoglobin