title: Mutational analysis of the beta-catenin gene in gastric carcinomas.
abstract: Previous studies reported that mutation of the adenomatous polyposis coli (APC) gene was not observed in the majority of gastric cancers. To evaluate the role of the APC/beta-catenin/Tcf pathway, we analyzed mutations in the beta-catenin gene and the accumulation of beta-catenin protein in gastric carcinomas. An interstitial deletion spanning exon 3 of the beta-catenin gene was observed in 1 of 13 gastric cancer cell lines. No missense mutation was found in these 13 cell lines. Nuclear and/or cytoplasmic localization of beta-catenin was observed in 16 of 70 primary gastric carcinomas by immunohistochemistry, while we found no mutations in exon 3 in 35 carcinoma tissues available for PCR amplification. Our findings suggest that somatic mutations of the beta-catenin gene are rare in human gastric carcinomas and that accumulation of normal beta-catenin protein in a subset of gastric cancers may be due to other mechanisms of its activation.
authors: Department of Molecular Biology, Cancer Research Institute, Sapporo Medical University School of Medicine, Sapporo, Japan.
authors: Y
authors: Y
authors: Sasaki
authors: I
authors: I
authors: Morimoto
authors: M
authors: M
authors: Kusano
authors: M
authors: M
authors: Hosokawa
authors: F
authors: F
authors: Itoh
authors: K
authors: K
authors: Yanagihara
authors: K
authors: K
authors: Imai
authors: T
authors: T
authors: Tokino
mesh_terms: D025601:Adenomatous Polyposis Coli Protein; D002277:Carcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000096:biosynthesis* / Q000235:genetics*; D004252:DNA Mutational Analysis; D004847:Epithelial Cells / Q000378:metabolism; D005091:Exons; D017353:Gene Deletion; D006801:Humans; D007150:Immunohistochemistry; D008957:Models, Genetic; D009154:Mutation; D016133:Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D015534:Trans-Activators; D014407:Tumor Cells, Cultured; D051176:beta Catenin
chemical_list: D025601:Adenomatous Polyposis Coli Protein; C495270:CTNNB1 protein, human; D003598:Cytoskeletal Proteins; D015534:Trans-Activators; D051176:beta Catenin