A new monoclonal anti-CD3epsilon antibody reactive on paraffin sections.
We generated a new monoclonal antibody (MAb), F7.2.38, by immunizing mice with CD3varepsilongammadelta/CD3omega complexes purified from human T-cells by OKT3 MAb-Sepharose affinity chromatography. Immunoprecipitation experiments and Western blotting analysis showed that MAb F7.2.38 recognized the CD3varepsilon chain in CD3varepsilon cDNA-transfected FOX B-cells and in various T-cell lines. Using flow cytometry on permeabilized or intact cells, the epitope was found to be located in the cytoplasmic tail of the CD3varepsilon chain. Immunohistochemical staining on paraffin-embedded sections showed that the reactivity of MAb F7.2.38 was comparable to that of the commercially available anti-CD3varepsilon polyclonal antibody. Of the 52 well-characterized T-cell lymphomas, 41 were positive for F7. 2.38 (79%), whereas all 37 B-cell lymphomas and 69 non-lymphoid tumors were unreactive. This new anti-CD3varepsilon antibody would be particularly useful for phenotyping T-cell lymphomas on routinely processed paraffin-embedded tissue sections.
Alibaud
L
L
Unité de Physiopathologie Cellulaire et Moléculaire, CNRS-UPR 2163, Institut Claude de Preval, IFR 30, Chu de Purpan, Toulouse, France.
Llobera
R
R
Al Saati
T
T
March
M
M
Delsol
G
G
Rubin
B
B
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D015153:Blotting, Western; D017252:CD3 Complex; D002460:Cell Line; D000939:Epitopes; D005404:Fixatives; D005434:Flow Cytometry; D005557:Formaldehyde; D005629:Frozen Sections; D006689:Hodgkin Disease / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008228:Lymphoma, Non-Hodgkin / Q000737:chemistry / Q000473:pathology; D051379:Mice; D016612:Paraffin Embedding; D011233:Precipitin Tests; D011817:Rabbits; D011948:Receptors, Antigen, T-Cell / Q000032:analysis / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology; D016707:Tissue Fixation
D000911:Antibodies, Monoclonal; D017252:CD3 Complex; C115335:CD3E protein, human; D000939:Epitopes; D005404:Fixatives; D011948:Receptors, Antigen, T-Cell; D005557:Formaldehyde