The survival, function, and hemolysis of human RBCs stored at 4 degrees C in additive solution (AS-1, AS-3, or AS-5) for 42 days and then biochemically modified, frozen, thawed, washed, and stored at 4 degrees C in sodium chloride and glucose solution for 24 hours.
BACKGROUND
A study was done to assess the quality of RBCs stored at 4 degrees C in AS-1, AS-3, or AS-5 for 42 days before biochemical modification and freezing.


METHODS
RBCs were stored at 4 degrees C for 42 days in AS-1, AS-3, or AS-5 and then biochemically modified with pyruvate, inosine, phosphate, and adenine solution (Rejuvesol), frozen with 40-percent (wt/vol) glycerol, and stored at -80 degrees C for at least 2 months. The RBCs were deglycerolized by the use of a cell washer (Haemonetics 115), and stored for 24 hours at 4 degrees C in a 0.9-percent sodium chloride and 0.2-percent glucose solution before the autologous transfusion.


RESULTS
The mean freeze-thaw-wash recovery process produced RBC recovery values of 85 percent, with the mean 24-hour posttransfusion survival at 75 percent, and the mean index of therapeutic effectiveness at 64 percent for the RBCs stored at 4 degrees C in AS-1, AS-3, or AS-5 for 42 days before biochemical modification and freezing. All the units exhibited normal or slightly higher than normal 2,3 DPG levels after deglycerolization and postwash storage at 4 degrees C for 24 hours.


CONCLUSIONS
RBCs stored in AS-1, AS-3, or AS-5 at 4 degrees C for 42 days and then biochemically modified with pyruvate, inosine, phosphate, and adenine and glycerolized, frozen, washed, and stored at 4 degrees C for 24 hours before autologous transfusion had acceptable in vitro and in vivo measurements.
Valeri
C R
CR
Naval Blood Research Laboratory, Boston University School of Medicine, Boston, Massachusetts 02118, USA.
Pivacek
L E
LE
Cassidy
G P
GP
Ragno
G
G
D000225:Adenine / Q000494:pharmacology*; D000328:Adult; D001782:Blood Donors; D001793:Blood Preservation; D015925:Cryopreservation; D004905:Erythrocyte Aging / Q000502:physiology*; D004912:Erythrocytes / Q000502:physiology*; D017110:Freeze Substitution / Q000379:methods*; D005947:Glucose / Q000494:pharmacology*; D006461:Hemolysis / Q000502:physiology*; D006801:Humans; D008297:Male; D008353:Mannitol / Q000494:pharmacology*; D012965:Sodium Chloride / Q000494:pharmacology*; D013997:Time Factors
C037839:ADSOL; D008353:Mannitol; D012965:Sodium Chloride; D005947:Glucose; D000225:Adenine