Reduction of hepatic reperfusion injury by antithrombin III and aprotinin.
Disturbance in hepatic microcirculation and leucocyte-endothelium interaction after warm ischaemia represents one of the leading mechanisms for postoperative organ dysfunction. Recent studies have shown that pretreatment with antithrombin III (AT III) and aprotinin reduces the leucocyte-endothelium interaction in ischaemic small intestine and during extracorporal circulation in cardiac surgery. Standardized warm hepatic ischaemia and intravital fluorescence videomicroscopy was performed in an experimental study with rats. Animals were pretreated with AT III or aprotinin. Analysis of intravital videomicroscopy showed that the hepatic microcirculation after warm hepatic ischaemia in rats was significantly enhanced by AT III and aprotinin, most likely by reducing the leucocyte-endothelium interaction. We concluded that drug application before the Pringle manoeuvre might reduce the reperfusion damage after liver resection.
Maksan
S M
SM
Department of Surgery, University of Heidelberg, Germany.
Maksan
M O
MO
Gebhard
M M
MM
Herfarth
C
C
Klar
E
E
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000990:Antithrombin III / Q000494:pharmacology*; D007611:Aprotinin / Q000494:pharmacology*; D001219:Aspartate Aminotransferases / Q000097:blood; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D004730:Endothelium, Vascular / Q000187:drug effects / Q000502:physiology; D008099:Liver / Q000098:blood supply / Q000187:drug effects / Q000502:physiology*; D008102:Liver Circulation / Q000187:drug effects / Q000502:physiology*; D008297:Male; D018715:Microscopy, Video; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*; D014699:Venules / Q000502:physiology
D000990:Antithrombin III; D007611:Aprotinin; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase