title: Fibroblast contractility: usual interstitial pneumonia and nonspecific interstitial pneumonia.
abstract: The aim of this study was to compare the function of lung fibroblasts obtained from surgically biopsied specimens of patients with idiopathic pulmonary fibrosis/usual interstitial pneumonia (UIP; n = 5), nonspecific interstitial pneumonia (NSIP; n = 5), and normal parts of surgically resected lungs (control; n = 5). The results showed that (1) fibroblasts obtained from UIP showed increased contractility compared with those obtained from NSIP or controls (UIP, 72.7 +/- 6.21%; NSIP, 32.8 +/- 5.46; controls, 28.5 +/- 3.51, p < 0.01 in UIP versus NSIP or control); (2) this increase in contractility was consistent with enhanced F-actin content in fibroblasts; (3) conditioned media from UIP fibroblast cultures enhanced control fibroblast contractility, whereas those obtained from NSIP or controls did not; (4) the 180 and 25 kD products representing the contractility in conditioned media were identified as fibronectin (ED-A domain) and TGF-beta1 by immunoblots, respectively; (5) the UIP-conditioned media contained higher amounts of fibronectin or TGF-beta 1 (fibronectin: UIP 289 +/- 47.1 ng/ml, NSIP 121 +/- 23.0, control 118 +/- 16.0; TGF-beta1: UIP 798 +/- 119 pg/ml, NSIP 246 +/- 69.1, control 247 +/- 53.6, p < 0.01 in UIP versus NSIP or control); () the contractility positively correlated with the amount of either fibronectin (r = 0.867, p < 0.001, n = 15) or TGF-beta 1 (r = 0.939, p < 0.001, n = 15), respectively. Thus, UIP fibroblasts showed greater contractility than did NSIP fibroblasts and up-regulated control fibroblasts.
authors: Department of Respiratory Medicine, Graduate School of Medicine, Kyoto University, Japan.
authors: H
authors: H
authors: Miki
authors: T
authors: T
authors: Mio
authors: S
authors: S
authors: Nagai
authors: Y
authors: Y
authors: Hoshino
authors: T
authors: T
authors: Nagao
authors: M
authors: M
authors: Kitaichi
authors: T
authors: T
authors: Izumi
mesh_terms: D000199:Actins / Q000032:analysis; D000230:Adenocarcinoma / Q000473:pathology; D000704:Analysis of Variance; D001706:Biopsy; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D005260:Female; D005347:Fibroblasts / Q000737:chemistry / Q000502:physiology*; D005782:Gels; D006222:Hamartoma / Q000473:pathology; D006801:Humans; D008168:Lung / Q000473:pathology; D008171:Lung Diseases / Q000473:pathology; D017563:Lung Diseases, Interstitial / Q000378:metabolism / Q000503:physiopathology*; D008175:Lung Neoplasms / Q000473:pathology; D008297:Male; D008875:Middle Aged; D016212:Transforming Growth Factor beta / Q000032:analysis; D053773:Transforming Growth Factor beta1
chemical_list: D000199:Actins; D016895:Culture Media, Serum-Free; D005782:Gels; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1