title: In vitro selection of hematoporphyrin binding DNA aptamers.
abstract: DNA aptamers that bind to hematoporphyrin IX (HPIX) were isolated using an in vitro selection technique. Most aptamers obtained after the 7th and 10th rounds contained guanine-rich sequences. Binding assay using fluorescence polarization technique and structural analysis by CD spectra revealed that the parallel guanine-quartet structure of the aptamer participates in the recognition of HPIX.
authors: Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Japan.
authors: A
authors: A
authors: Okazawa
authors: H
authors: H
authors: Maeda
authors: E
authors: E
authors: Fukusaki
authors: Y
authors: Y
authors: Katakura
authors: A
authors: A
authors: Kobayashi
mesh_terms: D001483:Base Sequence; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D005454:Fluorescence Polarization; D006415:Hematoporphyrins / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011485:Protein Binding
chemical_list: D006415:Hematoporphyrins; D004247:DNA