title: Hunting the subset-specific genes of neuroblastoma: expression profiling and differential screening of the full-length-enriched oligo-capping cDNA libraries.
abstract: BACKGROUND
  Neuroblastoma (NBL) has a distinct nature in different prognostic subgroups.
  
  
  METHODS
  To understand the molecular mechanism of NBL's genesis and biology as well as that of the neural crest development, we constructed full-length-enriched cDNA libraries by an oligo-capping method from two different subsets of primary NBL, one with favorable biology and the other with MYCN amplification.
  
  
  RESULTS
  Sequencing analysis of these libraries revealed that the expression profile was markedly different between both subsets. To identify the genes differentially expressed between the subsets, semi-quantitative RT-PCR analyses are proceeding.
  
  
  CONCLUSIONS
  So far, 54 transcripts have been found to be expressed at high levels in favorable NBLs, and significantly at low levels in unfavorable NBLs.
authors: Division of Biochemistry, Chiba Cancer Center Research Institute, Chiba, Japan.
authors: M
authors: M
authors: Ohira
authors: T
authors: T
authors: Shishikura
authors: T
authors: T
authors: Kawamoto
authors: H
authors: H
authors: Inuzuka
authors: A
authors: A
authors: Morohashi
authors: H
authors: H
authors: Takayasu
authors: H
authors: H
authors: Kageyama
authors: N
authors: N
authors: Takada
authors: M
authors: M
authors: Takahashi
authors: S
authors: S
authors: Sakiyama
authors: Y
authors: Y
authors: Suzuki
authors: S
authors: S
authors: Sugano
authors: H
authors: H
authors: Kuma
authors: I
authors: I
authors: Nozawa
authors: A
authors: A
authors: Nakagawara
mesh_terms: D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D015723:Gene Library; D006801:Humans; D008403:Mass Screening; D009447:Neuroblastoma / Q000235:genetics*; D014407:Tumor Cells, Cultured