Antimalarial activities of peptide antibiotics isolated from fungi.
Malaria caused by Plasmodium falciparum is a major public health problem in the developing countries of the world. Clinical treatment of malaria has become complicated due to the occurrence of infections caused by drug resistant parasites. Secondary metabolites from fungi are an attractive source of chemotherapeutic agents. This work reports the isolation and in vitro antiplasmodial activities of peptide antibiotics of fungal origin. The three peptide antibiotics used in this study were efrapeptins, zervamicins, and antiamoebin. The high-performance liquid chromatography-purified peptides were characterized by nuclear magnetic resonance and mass spectral analysis. All three fungal peptides kill P. falciparum in culture with 50% inhibitory concentrations in the micromolar range. A possible mode of action of these peptide antibiotics on P. falciparum is presented.
Nagaraj
G
G
Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore 560 064, India.
Uma
M V
MV
Shivayogi
M S
MS
Balaram
H
H
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000962:Antimalarials / Q000494:pharmacology*; D001203:Ascomycota / Q000187:drug effects / Q000378:metabolism*; D004912:Erythrocytes / Q000469:parasitology; D006999:Hypocreales / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D054848:Peptaibols; D010455:Peptides; D010963:Plasmodium falciparum / Q000469:parasitology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000900:Anti-Bacterial Agents; D000962:Antimalarials; D054848:Peptaibols; D010455:Peptides; C006596:antiamoebin; C012953:efrapeptin