A method for the sequential study of eimerian chromosomes by light and electron microscopy.
In the present study, the authors describe a simple method to isolate chromosomes from eimerian oocysts and to submit them to sequential study by light and electron microscopy. This method includes a reliable and reproducible technique for transferring eimerian chromosomes from slides to grid that fulfills the essential requirements for generalized use in cytogenetics. In addition, this method overcomes the difficulty of the resistance of protozoan oocysts to disruption and permits the release of intact meiotic chromosomes. The observation by the authors of synaptonemal complexes in meiotic chromosomes of different Eimeria species by applying the above-mentioned method to oocysts revealed its importance to future applications.
del Cacho
E
E
Department of Animal Pathology, Faculty of Veterinary Sciences, Miguel Servet 177, 50013, Zaragoza, Spain.
Gallego
M
M
Monteagudo
L
L
Lopez-Bernad
F
F
Quilez
J
J
Sanchez-Acedo
C
C
D000818:Animals; D002875:Chromosomes / Q000737:chemistry* / Q000648:ultrastructure; D004539:Eimeria / Q000235:genetics*; D005821:Genetic Techniques / Q000662:veterinary*; D008853:Microscopy / Q000662:veterinary; D008854:Microscopy, Electron / Q000662:veterinary