Phagocytosis and chemiluminescence response of granulocytes to monodisperse latex particles of varying sizes and surface coats.
The response of human granulocytes to polystyrene latex beads of diameter 0.1-7 microm was measured by luminol-dependent chemiluminescence. In all instances, the response to beads of 3-7 microm was definitely higher than with smaller beads. In protein-free medium, the chemiluminescence response was slow compared to that of opsonized zymosan, and the highest response was only 9% of the response to opsonized zymosan. Scanning electron microscopy showed that granulocytes in suspension bound the particles, occasionally by extending rope-like protrusions. When the beads were coated with albumin, the chemiluminescence diminished to about 1/3 of that seen with uncoated beads; however, preincubating the beads in serum led to a large increase with beads of 1.1 microm (to 25% of the maximal response to opsonized zymosan) and 3.19 microm (to 42%), but with the smallest beads, no increase was noted. "Priming" of the cells with tumor necrosis factor-alpha caused a further increase with serum-coated beads. When uncoated beads of 1.1 microm were tested with "primed" cells, there was an increase of 6 times in the chemiluminescence compared to un-"primed" cells.
Volle
J M
JM
Nycomed Amersham, Torshov, Oslo, Norway.
Tolleshaug
H
H
Berg
T
T
D001769:Blood; D020099:Coated Materials, Biocompatible; D006098:Granulocytes / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D006801:Humans; D007202:Indicators and Reagents; D007840:Latex / Q000494:pharmacology*; D008163:Luminescent Measurements; D008165:Luminol; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D010316:Particle Size; D010587:Phagocytosis / Q000502:physiology*; D012709:Serum Albumin / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D020099:Coated Materials, Biocompatible; D007202:Indicators and Reagents; D007840:Latex; D012709:Serum Albumin; D014409:Tumor Necrosis Factor-alpha; D008165:Luminol