Evidence for the presence of two homer 1 transcripts in skeletal and cardiac muscles.
A family of proteins, Homers 1, 2 and 3, involved in activity-dependent control of signal transduction has been recently described in neurons [Xiao, B., Tu, C. J., Petralia, R. S., Yuan, J. P., Doan, A., Breder, C. D., Ruggiero, A., Lanahan, A. A., Wenthold, R. J., and Worley, P. F. (1998) Homer regulates the association of group 1 metabotropic glutamate receptors with multivalent complexes of Homer-related, synaptic proteins. Neuron 21, 707-716]. By RT-PCR and RNasePA, mRNAs transcripts for Homer 1a and Homer 1c, but not Homer 1b, are detected in both skeletal and cardiac muscles of the rat. Full-length cloning of Homer 1a and Homer 1c cDNAs has been accomplished: There is no tissue specificity, by comparing skeletal muscle, cardiac muscle and cerebellum, and there are a few species-specific base substitutions, by comparing rat and mouse sequences. The regulatory mechanism exerted via transition of Homer 1 isoform composition may be operative in striated muscles.
Sandonà
D
D
Centro di Studio per la Biologia e la Fisiopatologia Muscolare del CNR, Dipartimento di Scienze Biomediche Sperimentali dell'Università di Padova, viale G. Colombo 3, Padua, 35121, Italy.
Tibaldo
E
E
Volpe
P
P
D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002531:Cerebellum / Q000378:metabolism; D018388:Codon, Terminator; D006325:Heart Atria; D006352:Heart Ventricles; D000072233:Homer Scaffolding Proteins; D051379:Mice; D008969:Molecular Sequence Data; D018656:Muscle Fibers, Fast-Twitch / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D009479:Neuropeptides / Q000235:genetics*; D009928:Organ Specificity; D020033:Protein Isoforms / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
D002352:Carrier Proteins; D018388:Codon, Terminator; D000072233:Homer Scaffolding Proteins; D009479:Neuropeptides; D020033:Protein Isoforms; D012333:RNA, Messenger