title: Overexpression of heme oxygenase-1 protects allogeneic thyroid grafts from rejection in naive mice.
abstract: BACKGROUND
  Endocrine allografts are an option for the treatment of endocrine failure.
  
  
  METHODS
  One lobe of the thyroid was transplanted under the kidney capsule.
  
  
  RESULTS
  C57BL/10 (H2(b)) thyroids were rejected in naive CBA (H2(k)) mice within 14 days after transplantation. When mice were treated with anti-CD4 monoclonal antibodies (mAb), all grafts survived for more than 60 days. The first grafts still survived after second C57BL/10 or Balb/c (H2(d)) thyroid grafts that were transplanted into the same recipients were rejected acutely, which suggests that the primary grafts were modified under anti-CD4 mAb treatment. To confirm this hypothesis, C57BL/10 thyroid grafts from anti-CD4 mAb-treated mice were retransplanted. All grafts survived in naive mice; this correlated with the overexpression of heme oxygenase-1 (HO-1) in the grafts. Next, an inhibitor of HO-1 (zinc protoporphyrin) or control compound (copper protoporphyrin) was injected intraperitoneally after transplantation of C57BL/10 thyroid grafts into the primary CBA recipients that had been treated with anti-CD4 mAb. The grafts in mice that had been treated with zinc protoporphyrin, but not copper protoporphyrin, were rejected when retransplanted to naive recipients.
  
  
  CONCLUSIONS
  Overexpression of HO-1 correlated with the protection of fully allogeneic thyroid grafts from rejection when retransplanted into naive recipients.
authors: First Department of Surgery, Teikyo University, Tokyo, Japan.
authors: M
authors: M
authors: Niimi
authors: M
authors: M
authors: Takashina
authors: H
authors: H
authors: Takami
authors: Y
authors: Y
authors: Ikeda
authors: T
authors: T
authors: Shatari
authors: K
authors: K
authors: Hamano
authors: K
authors: K
authors: Esato
authors: K
authors: K
authors: Matsumoto
authors: K
authors: K
authors: Kameyama
authors: S
authors: S
authors: Kodaira
authors: K J
authors: KJ
authors: Wood
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use; D015704:CD4 Antigens / Q000502:physiology; D003167:Complement Activation; D006084:Graft Rejection / Q000517:prevention & control*; D006419:Heme Oxygenase (Decyclizing) / Q000502:physiology*; D051547:Heme Oxygenase-1; D008565:Membrane Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D013961:Thyroid Gland / Q000637:transplantation*; D014184:Transplantation, Homologous
chemical_list: D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D008565:Membrane Proteins; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1; C496542:Hmox1 protein, mouse