Regulation of the rat liver carnitine palmitoyltransferase I gene transcription by thyroid hormone.
L-CPT I isotype is the main locus of control for liver LCFA oxidation. T3 levels have been described as controlling L-CPT I gene expression, and in this paper we demonstrate that rat liver CPT I promoter responds to T3. Using deleted reporter constructs we located the thyroid hormone-responsive element between -2935 and -2918, consisting of a DR4. This response is mediated by the binding of the thyroid to this sequence as a monomer, homodimer, or heterodimer with RXR.
Barrero
M J
MJ
Department of Biochemistry and Molecular Biology, School of Pharmacy, E-08028 Barcelona, Spain.
Marrero
P F
PF
Haro
D
D
D000818:Animals; D001483:Base Sequence; D002334:Carnitine O-Palmitoyltransferase / Q000235:genetics*; D002460:Cell Line; D017931:DNA Primers; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D011401:Promoter Regions, Genetic; D051381:Rats; D014158:Transcription, Genetic / Q000502:physiology*; D014284:Triiodothyronine / Q000502:physiology*
D017931:DNA Primers; D014284:Triiodothyronine; D002334:Carnitine O-Palmitoyltransferase