Nonradioactive mRNA differential display in polyacrylamide mini-gels.
Nonradioactive methods in comparison to those using radionuclides are now becoming popular because of safety, environmental, and cost concerns. The modified technique described here is much faster, easier, and safer than the conventional procedure utilizing radioactive material and large-formatted polyacrylamide gel, or other nonradioactive DD techniques. Also, it maintains good reproducibility and specificity. This DD protocol offers a rapid and reliable nonradioactive method for comparing gene expression patterns in various research.
Lee
S H
SH
Department of Biochemistry, College of Medicine, Soonchunhyang University, Cheon-An, Korea. L1037624@asan.sch.ac.kr
Shiao
Y H
YH
Kasprzak
K S
KS
D000818:Animals; D015152:Blotting, Northern; D016466:CHO Cells; D006224:Cricetinae; D018076:DNA, Complementary; D004591:Electrophoresis, Polyacrylamide Gel; D020869:Gene Expression Profiling / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis; D012680:Sensitivity and Specificity; D012835:Silver Nitrate / Q000737:chemistry
D018076:DNA, Complementary; D012333:RNA, Messenger; D012835:Silver Nitrate