Production of eicosapentaenoic acid by a recombinant marine cyanobacterium, Synechococcus sp.
The eicosapentaenoic acid (EPA) synthesis gene cluster from an EPA-producing bacterium, Shewanella sp. SCRC-2738, was cloned into a broad-host range vector, pJRD215, and then introduced into a marine cyanobacterium, Synechococcus sp. NKBG15041c, by conjugation. The transconjugant cyanobacteria produced 3.7 +/- 0.2% (2.24 +/- 0.13 mg/L) EPA (n-3) and 2.5 +/- 0.2% (1.49 +/- 0.06 mg/L) eicosatetraenoic acid (n-3) of the total fatty acids when the cells were cultured at 23 degrees C at a light intensity of 1,000-1,500 Lux. The EPA and eico-satetraenoic acid contents of the cells were increased to 4.6 +/- 0.6% (3.86 +/- 1.11 mg/L) and 4.7 +/- 0.3% (3.86 +/- 0.82 mg/L), and 7.5 +/- 0.3% (1.76 +/- 0.10 mg/L) and 5.1 +/- 0.2% (1.19 +/- 0.06 mg/L) when they were cultured at low temperature (18 degrees C) and at lower light intensity (40 Lux), respectively.
Yu
R
R
Sagami Chemical Research Center, Kanagawa, Japan. ryuscrc@ce.mbn.or.jp
Yamada
A
A
Watanabe
K
K
Yazawa
K
K
Takeyama
H
H
Matsunaga
T
T
Kurane
R
R
D020591:Alteromonas / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D015118:Eicosapentaenoic Acid / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D004926:Escherichia coli; D005227:Fatty Acids / Q000096:biosynthesis / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D018014:Gene Transfer Techniques; D008027:Light; D008957:Models, Genetic; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids / Q000378:metabolism; D013696:Temperature; D019076:Transgenes
D005227:Fatty Acids; D004247:DNA; D015118:Eicosapentaenoic Acid