HSP70 from Trypanosoma cruzi is endowed with specific cell proliferation potential leading to apoptosis.
The Trypanosoma cruzi HSP70 recombinant protein has the capacity to stimulate splenocytes or lymph node cells from naive mice in a non-haplotype-restricted way. The proliferative response is abolished by proteinase K digestion and by specific anti-HSP70 antibodies. The induced stimulation index was maximal after 24 h of incubation with the protein. This stimulation leads to cell death in a Fas-Fas ligand-independent way. The phenotype of the expanded cells was CD3(+) TCRalphass(+) CD4(+). HSP70-responsive cells express a broad range of cytokines including IFN-gamma, IL-2 and tumor necrosis factor-alpha. After 48 h of incubation with HSP70 there was a significant increase in relative intracellular levels of CD3 TCRalphass receptors. The expanded CD4(+) cell population expressed CD25; however, in contrast to concanavalin A-treated culture, delayed CD44 expression was observed.
Marañón
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C
Departamento de Biología Molecular, Instituto de Parasitología y Biomedicina 'López Neyra', CSIC, Calle Ventanilla 11, 18001 Granada, Spain.
Planelles
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L
Alonso
C
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López
M C
MC
D000818:Animals; D000953:Antigens, Protozoan / Q000494:pharmacology*; D017209:Apoptosis; D017252:CD3 Complex / Q000032:analysis; D015704:CD4 Antigens / Q000032:analysis; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology; D019286:Endopeptidase K / Q000494:pharmacology; D053222:Fas Ligand Protein; D018840:HSP70 Heat-Shock Proteins / Q000494:pharmacology*; D008198:Lymph Nodes / Q000187:drug effects / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects*; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis; D011994:Recombinant Proteins / Q000494:pharmacology; D013154:Spleen / Q000187:drug effects / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D013997:Time Factors; D014349:Trypanosoma cruzi / Q000276:immunology*; D019014:fas Receptor / Q000378:metabolism
D000953:Antigens, Protozoan; D017252:CD3 Complex; D015704:CD4 Antigens; D016207:Cytokines; D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; D018840:HSP70 Heat-Shock Proteins; D008562:Membrane Glycoproteins; D016693:Receptors, Antigen, T-Cell, alpha-beta; D011994:Recombinant Proteins; D019014:fas Receptor; D019286:Endopeptidase K