Genotyping of verocytotoxin-producing Escherichia coli O157: comparison of isolates of a prevalent phage type by fluorescent amplified-fragment length polymorphism and pulsed-field gel electrophoresis analyses.
We applied the high-resolution genotyping technique fluorescent amplified-fragment length polymorphism (FAFLP) analysis to 71 isolates of a single phage type (PT8) of pulsed-field gel electrophoresis (PFGE)-characterized verocytotoxin-producing Escherichia coli O157. Twenty-seven similar, but not identical, groupings were defined by both FAFLP analysis and the PFGE profiles. Given the FAFLP analysis conditions described here, these two methods exhibited equivalent discriminatory powers.
Smith
D
D
Molecular Biology Unit, Virus Reference Division, Central Public Health Laboratory, London NW9 5HT, United Kingdom.
Willshaw
G
G
Stanley
J
J
Arnold
C
C
D001434:Bacteriophage Typing; D016521:Electrophoresis, Gel, Pulsed-Field; D019453:Escherichia coli O157 / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity; D005456:Fluorescent Dyes; D005784:Gene Amplification; D005838:Genotype; D006801:Humans; D022603:Shiga Toxins / Q000096:biosynthesis*
D005456:Fluorescent Dyes; D022603:Shiga Toxins