title: Altered superantigenic ligands demonstrate the quantitative nature of T-cell activation.
abstract: In a recent study, a superantigen mutated in the TCR binding site (staphylococcal enterotoxin B (SEB)delta61Y) was described, which behaved as a partial agonist for a Vbeta17-expressing T-cell clone. Evidence is now presented to demonstrate that there is distinct heterogeneity in the response of primary T cells to this protein. Some Vbeta17 T cells responded to SEBdelta61Y by modulating surface receptor expression consistent with activation, and by proliferating. Other Vbeta17 T cells did not proliferate, nor did they display a receptor expression phenotype consistent with activation. However, when repeatedly exposed to the altered superantigen, some of these non-responders entered cell cycle. This pattern of responses was not recapitulated by providing additional costimulation via CD28, although such treatment did induce some of the 'unresponsive' Vbeta17 T cells to upregulate the IL-2 receptor, indicative of partial activation. It was also found that the heterogeneous pattern could be replicated using very low doses of native SEB. The data are discussed in the context of models of T-cell activation in which differences in TCR ligand affinity and dose determine qualitatively different response phenotypes.
authors: Department of Haematology, Institute of Medical and Veterinary Science/Hanson Centre for Cancer Research, Adelaide, South Australia, Australia. john.hayball@imvs.sa.gov.au
authors: J D
authors: JD
authors: Hayball
authors: R A
authors: RA
authors: Lake
mesh_terms: D018106:CD28 Antigens / Q000276:immunology; D015536:Down-Regulation; D004768:Enterotoxins / Q000235:genetics / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000276:immunology; D017493:Leukocyte Common Antigens / Q000276:immunology; D008024:Ligands; D008213:Lymphocyte Activation; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000096:biosynthesis*; D015375:Receptors, Interleukin-2 / Q000276:immunology; D017384:Sequence Deletion; D018089:Superantigens / Q000235:genetics / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology; D015854:Up-Regulation
chemical_list: D018106:CD28 Antigens; D004768:Enterotoxins; D007135:Immunoglobulin Variable Region; D008024:Ligands; D016693:Receptors, Antigen, T-Cell, alpha-beta; D015375:Receptors, Interleukin-2; D018089:Superantigens; C031818:enterotoxin B, staphylococcal; D017493:Leukocyte Common Antigens