Evidence for existence of immobilization stress-inducible semicarbazide-sensitive amine oxidase inhibitor in rat brain cytosol.
An endogenous inhibitor of semicarbazide-sensitive amine oxidase (SSAO) was separated by gel filtration from 105000xg supernate in rat brain cytosol following immobilization stress (IMMO). The molecular weight of this inhibitor was estimated to be 500-700 by gel filtration. This inhibitor was proved to be heat-stable resistant to protease treatment. These results suggest that this inhibitor is induced by IMMO. SSAO activity in rat brain might be regulated by the level of this inhibitor.
Obata
T
T
Department of Pharmacology, Oita Medical University, Hasama-machi, 879-5593, Oita, Japan. tobata@oita-med.ac.jp
Yamanaka
Y
Y
D006631:Amine Oxidase (Copper-Containing) / Q000037:antagonists & inhibitors*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002850:Chromatography, Gel; D003600:Cytosol / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000302:isolation & purification* / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D012149:Restraint, Physical; D013315:Stress, Psychological / Q000378:metabolism*
D004791:Enzyme Inhibitors; D006631:Amine Oxidase (Copper-Containing)