Applications of transketolases in organic synthesis.
The enzyme transketolase has been employed as a catalyst for asymmetric carbon-carbon bond formation in the synthesis of biologically important molecules. A number of important parameters have been addressed including substrate specificity, over-expression of the protein in suitable host systems, scale-up of the reaction and use of transketolase in multi-enzyme experiments. X-ray structural studies have been used to probe the origin of the asymmetry of the carbon-carbon bond-forming process.
Turner
N J
NJ
Department of Chemistry, University of Edinburgh, King's Buildings, West Mains Road, Edinburgh EH9 3JJ, UK. n.j.turner@ed.ac.uk
D002625:Chemistry, Organic / Q000379:methods*; D009930:Organic Chemicals / Q000378:metabolism*; D014174:Transketolase / Q000378:metabolism*
D009930:Organic Chemicals; D014174:Transketolase