Enzymatic versus spontaneous S-methyl thioester synthesis in Geotrichum candidum.
The synthesis of short chain S-methyl thioesters was investigated in Geotrichum candidum strain GcG. The results indicated the involvement of an enzymatic reaction in this microorganism that led to the synthesis of S-methyl thioacetate (MTA) when methanethiol and acetyl-CoA were used as substrates. MTA was generated from these substrates by enzymatic or spontaneous reactions, whose relative importance depended largely on pH and temperature. For longer chain acyl-CoA compounds (C3 to C6), thioester synthesis was primarily spontaneous. Short chain fatty acid activation by a CoA residue probably is a prerequisite for the synthesis of S-methyl thioesters.
Helinck
S
S
Institut National de la Recherche Agronomique, Laboratoire de Génie et Microbiologie des Procédés Alimentaires, Thiverval-Grignon, France.
Spinnler
H E
HE
Parayre
S
S
Dame-Cahagne
M
M
Bonnarme
P
P
D000085:Acetates / Q000737:chemistry* / Q000378:metabolism*; D000105:Acetyl Coenzyme A / Q000737:chemistry / Q000378:metabolism; D005848:Geotrichum / Q000737:chemistry* / Q000201:enzymology* / Q000254:growth & development; D006863:Hydrogen-Ion Concentration; D013438:Sulfhydryl Compounds / Q000737:chemistry / Q000378:metabolism; D013455:Sulfur / Q000378:metabolism
D000085:Acetates; D013438:Sulfhydryl Compounds; C005231:methylmercaptan; D013455:Sulfur; D000105:Acetyl Coenzyme A; C026500:methyl thioacetate