Expression of heterologous promoters in Lutzomyia longipalpis and Phlebotomus papatasi (Diptera: Psychodidae) cell lines.
To establish a transient expression system for genes introduced into sand fly cell lines, we tested the expression of the luciferase reporter gene under control of different promoters. Towards this end, we lipofected cell lines obtained from New and Old World sand flies, LL-5 from Lutzomyia longipalpis Lutz & Neiva and PP-9 from Phlebotomus papatasi Scopoli, respectively. The relative levels of luciferase expression were studied under control of Drosophila melanogaster Meigen heat shock protein 70 (hsp70), human cytomegalovirus, simian virus 40 or Junonia coenia (Hübner) densovirus (P9) promoters. The Drosophila heat shock protein 70 promoter, originating from insect genes, functioned as a strong promoter in both cell lines. Promoters from the different virus genes also were capable of driving transgene expression in both cell lines.
Saraiva
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Laboratory of Parasitic Disease, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 9000 Rockville Pike, Building 4, Room 126, Bethesda, MD, USA.
Fampa
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Cedeno
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Bergoin
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Mialhe
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Miller
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LH
D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods*; D003587:Cytomegalovirus / Q000235:genetics; D017990:Densovirus / Q000235:genetics; D004331:Drosophila melanogaster; D015870:Gene Expression; D017930:Genes, Reporter; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D006801:Humans; D008156:Luciferases / Q000235:genetics*; D010691:Phlebotomus / Q000166:cytology*; D011401:Promoter Regions, Genetic; D011576:Psychodidae / Q000166:cytology*; D013539:Simian virus 40 / Q000235:genetics
D018840:HSP70 Heat-Shock Proteins; D008156:Luciferases