title: Physiochemical properties of rat liver mitochondrial ribosomes.
abstract: In the present study, the physiochemical properties of rat liver mitochondrial ribosomes were examined and compared with Escherichia coli ribosomes. The sedimentation and translational diffusion coefficients as well as the molecular weight and buoyant density of rat mitochondrial ribosomes were determined. Sedimentation coefficients were established using the time-derivative algorithm (Philo, J. S. (2000) Anal. Biochem. 279, 151-163). The sedimentation coefficients of the intact monosome, large subunit, and small subunit were 55, 39, and 28 S, respectively. Mitochondrial ribosomes had a particle composition of 75% protein and 25% RNA. The partial specific volume was 0.688 ml/g, as determined from the protein and RNA composition. The buoyant density of formaldehyde-fixed ribosomes in cesium chloride was 1.41 g/cm(3). The molecular masses of mitochondrial and E. coli ribosomes determined by static light-scattering experiments were 3.57 +/- 0.14 MDa and 2.49 +/- 0.06 MDa, respectively. The diffusion coefficient obtained from dynamic light-scattering measurements was 1.10 +/- 0.01 x 10(-7) cm(2) s(-1) for mitochondrial ribosomes and 1.72 +/- 0.03 x 10(-7) cm(2) s(-1) for the 70 S E. coli monosome. The hydration factor determined from these hydrodynamic parameters were 4.6 g of water/g of ribosome and 1.3 g/g for mitochondrial and E. coli ribosomes, respectively. A calculated hydration factor of 3.3 g/g for mitochondrial ribosomes was also obtained utilizing a calculated molecular mass and the Svedberg equation. These measurements of solvation suggest that ribosomes are highly hydrated structures. They are also in agreement with current models depicting ribosomes as porous structures containing numerous gaps and tunnels.
authors: Department of Biochemistry, Wake Forest University School of Medicine, Winston-Salem, North Carolina 27157-1016, USA.
authors: V B
authors: VB
authors: Patel
authors: C C
authors: CC
authors: Cunningham
authors: R R
authors: RR
authors: Hantgan
mesh_terms: D000818:Animals; D004058:Diffusion; D004926:Escherichia coli / Q000648:ultrastructure; D008027:Light; D008854:Microscopy, Electron; D008930:Mitochondria, Liver / Q000648:ultrastructure*; D008970:Molecular Weight; D012335:RNA, Ribosomal / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012270:Ribosomes / Q000737:chemistry*; D012542:Scattering, Radiation
chemical_list: D012335:RNA, Ribosomal