title: Differential interleukin-8 response of intestinal epithelial cell line to reactogenic and nonreactogenic candidate vaccine strains of Vibrio cholerae.
abstract: In this study, we analyzed whether attachment of Vibrio cholerae vaccine strains to human intestinal epithelial cells can induce an interleukin-8 (IL-8) response. The IL-8 transcripts were detected by PCR amplification of reverse-transcribed mRNA, and the gene product secretion was measured by an enzyme-linked immunosorbent assay. Infection of monolayers of the undifferentiated HT29-18N2 cell line with reactogenic (JBK70 and 81) and nonreactogenic (CVD103HgR and 638) vaccine strains of V. cholerae resulted in markedly higher IL-8 expression by epithelial cells exposed to reactogenic strains than by cells exposed to the nonreactogenic strains. Additionally, epithelial cells produced IL-8 transcripts following stimulation with cholera vaccine strains in a concentration-dependent manner. These results represent a new insight into the inflammatory component of reactogenicity and could be used as a predictive marker of vaccine reactogenicity prior to human testing.
authors: Departamento de Genética, Centro Nacional de Investigaciones Científicas, La Habana, Cuba. boris@biocnic.cneuro.edu.cu
authors: B L
authors: BL
authors: Rodríguez
authors: A
authors: A
authors: Rojas
authors: J
authors: J
authors: Campos
authors: T
authors: T
authors: Ledon
authors: E
authors: E
authors: Valle
authors: W
authors: W
authors: Toledo
authors: R
authors: R
authors: Fando
mesh_terms: D001422:Bacterial Adhesion; D022121:Cholera Vaccines; D019073:HT29 Cells; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics*; D007413:Intestinal Mucosa / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014734:Vibrio cholerae / Q000502:physiology*
chemical_list: D022121:Cholera Vaccines; D016209:Interleukin-8; D012333:RNA, Messenger