The Schizosaccharomyces pombe GPI8 gene complements a Saccharomyces cerevisiae GPI8 anchoring mutant.
The final step in glycosylphosphatidylinositol (GPI) anchoring of cell surface proteins consists of a transamidation reaction, in which preassembled GPI donors are substituted for C-terminal signal sequences in nascent polypeptides. The Saccharomyces cerevisiae GPI8 gene (ScGPI8) encodes a protein which is involved in the GPI transamidation reaction. We have cloned and isolated the Schizosaccharomyces pombe GPI8 homologous gene (SpGPI8). The SpGPI8 gene encodes a protein of 411 amino acids with a calculated molecular weight of about 47 kDa. It shows 53.5% identity with the ScGPI8 and complements a S. cerevisiae GPI8 anchoring mutant.
Shams-Eldin
H
H
Medizinisches Zentrum für Hygiene und Med. Mikrobiologie, Institut für Virologie, Robert-Koch-Strasse 17, 35037 Marburg, Germany. shamseld@mailer.uni-marburg.de
Azzouz
N
N
Eckert
V
V
Blaschke
T
T
Kedees
M H
MH
Hübel
A
A
Schwarz
R T
RT
D000595:Amino Acid Sequence; D019881:Aminoacyltransferases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D020043:Genes, Essential; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D017261:Glycosylphosphatidylinositols / Q000378:metabolism*; D007294:Inositol / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D012568:Schizosaccharomyces / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins
D015815:Cell Adhesion Molecules; D017261:Glycosylphosphatidylinositols; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; C491191:gpi8 protein, S pombe; D007294:Inositol; D019881:Aminoacyltransferases; C103133:GPI8 protein, S cerevisiae; C023175:transamidases