title: D-glucose additive protects against osmotic-induced decrease in erythrocyte filterability.
abstract: Glucose has long-term effects on erythrocyte filterability owing to sorbitol accumulation and glycosylation of intracellular proteins, but glucose effects prior to these long-term metabolic consequences have not been studied to the same extent. D-glucose is osmotically inert in erythrocytes because of facilitated diffusion. Erythrocyte volume regulation, hemolysis and filterability were studied with reference to effects of glucose derivatives dissolved in water. Control situations were water alone or buffer. Salt-stock dilution by water, D-glucose, or 3-O-M-glucose in water (both 570 mmol/L additives) created volume increases of 4.0 +/- 0.2%, 3.4 +/- 0.5% and 3.3 +/- 0.2%, respectively, whereas L-glucose resulted in a 2.2 +/- 0.4% volume decrease (all p < 0.001 versus baseline, mean values +/- SEM). D-glucose at a final concentration 30 mmol/L did not display any osmotic properties. Despite erythrocyte swelling, electrolyte-free glucose did not induce any significant changes in filterability with either 3-microm or 5-microm filters. 3-O-M-glucose gave a 7.8 +/- 1.4% decrease in 3-microm filterability (mean value +/- SEM, p < 0.001), but not to the same extent as by water alone at the corresponding dilution rate (31.7 +/- 3.5%, mean value +/- SEM, p < 0.001). L-glucose caused a 2.0 +/- 0.8% decrease in filterability across the 5-microm (mean value +/- SEM, p < 0.05), but not with the 3-microm filters. Stressing the glucose transport system further, to simulate the water-like properties of electrolyte-free glucose during intravenous infusion, we found that glucose becomes osmotically active and prevents hemolysis at these extreme concentrations (> 114 mmol/L). We conclude that an in vivo concentration range of D-glucose protects the erythrocytes from a decreased filterability induced by osmotic swelling but without having any osmotic properties.
authors: Department of Surgical and Perioperative Sciences, Umeå University Hospital, Sweden. kristerlindmark@hotmail.com
authors: K
authors: K
authors: Lindmark
authors: K G
authors: KG
authors: Engström
mesh_terms: D002469:Cell Separation; D004912:Erythrocytes / Q000166:cytology*; D005947:Glucose / Q000494:pharmacology*; D006440:Hemofiltration; D009997:Osmotic Pressure
chemical_list: D005947:Glucose