Enhancement of nitroglycerin induced blood vessel relaxation in chronic renal failure model rats.
This study was designed to investigate the alternation of blood vessel relaxation in chronic renal failure (CRF) induced by adenine or partial-nephrectomy. The aorta was employed as the blood vessel material. CRF aorta relaxation in both adenine and partial nephrectomy induced rats increased when treated with glyceryl trinitrate (GTN). In the CRF animals, cGMP levels increased with the severity of CRF status. Aorta cytosolic glutathione S-transferase micro (GSTmicro) activity and enzyme contents increased with CRF. The effect of GTN on aortic vasorelaxation in both CRF statuses completely disappeared by the treatment with sodium nitoprusside. The effects of GTN were observed equally in both adenine- and partial nephrectomy-induced CRF rats. We concluded that alterations of aortic vasorelaxation by GTN in adenine- and partial nephrectomy-induced renal failure rats were caused by the enhancement of nitrogen monoxide production on the aortic blood vessel mediated by the induced GSTmicro in the aorta. This GSTmicro induction is peculiar to CRF since different CRF induction procedures produce the same results.
Kurata
N
N
Department of Pharmacology, School of Medicine, Showa University, Tokyo, Japan. noricats@med.showa-u.ac.jp
Nakamura
S
S
Mizumura
J
J
Sumi
M
M
Nishimura
Y
Y
Yamamoto
T
T
Yasuhara
H
H
D000225:Adenine; D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000201:enzymology; D001189:Aryl Hydrocarbon Hydroxylases; D001806:Blood Urea Nitrogen; D006152:Cyclic GMP / Q000096:biosynthesis; D051544:Cytochrome P-450 CYP3A; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004195:Disease Models, Animal; D005982:Glutathione Transferase / Q000378:metabolism*; D007676:Kidney Failure, Chronic / Q000201:enzymology / Q000209:etiology / Q000503:physiopathology*; D008297:Male; D009392:Nephrectomy; D005996:Nitroglycerin / Q000494:pharmacology*; D009599:Nitroprusside / Q000494:pharmacology; D010089:Oxidoreductases, N-Demethylating / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014664:Vasodilation / Q000187:drug effects*
D009599:Nitroprusside; D003577:Cytochrome P-450 Enzyme System; D001189:Aryl Hydrocarbon Hydroxylases; D051544:Cytochrome P-450 CYP3A; D010089:Oxidoreductases, N-Demethylating; D005982:Glutathione Transferase; D005996:Nitroglycerin; D006152:Cyclic GMP; D000225:Adenine