title: HSP70 from Trypanosoma cruzi is endowed with specific cell proliferation potential leading to apoptosis.
abstract: The Trypanosoma cruzi HSP70 recombinant protein has the capacity to stimulate splenocytes or lymph node cells from naive mice in a non-haplotype-restricted way. The proliferative response is abolished by proteinase K digestion and by specific anti-HSP70 antibodies. The induced stimulation index was maximal after 24 h of incubation with the protein. This stimulation leads to cell death in a Fas-Fas ligand-independent way. The phenotype of the expanded cells was CD3(+) TCRalphass(+) CD4(+). HSP70-responsive cells express a broad range of cytokines including IFN-gamma, IL-2 and tumor necrosis factor-alpha. After 48 h of incubation with HSP70 there was a significant increase in relative intracellular levels of CD3 TCRalphass receptors. The expanded CD4(+) cell population expressed CD25; however, in contrast to concanavalin A-treated culture, delayed CD44 expression was observed.
authors: Departamento de Biología Molecular, Instituto de Parasitología y Biomedicina 'López Neyra', CSIC, Calle Ventanilla 11, 18001 Granada, Spain.
authors: C
authors: C
authors: Marañón
authors: L
authors: L
authors: Planelles
authors: C
authors: C
authors: Alonso
authors: M C
authors: MC
authors: López
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000494:pharmacology*; D017209:Apoptosis; D017252:CD3 Complex / Q000032:analysis; D015704:CD4 Antigens / Q000032:analysis; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology; D019286:Endopeptidase K / Q000494:pharmacology; D053222:Fas Ligand Protein; D018840:HSP70 Heat-Shock Proteins / Q000494:pharmacology*; D008198:Lymph Nodes / Q000187:drug effects / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects*; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis; D011994:Recombinant Proteins / Q000494:pharmacology; D013154:Spleen / Q000187:drug effects / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D013997:Time Factors; D014349:Trypanosoma cruzi / Q000276:immunology*; D019014:fas Receptor / Q000378:metabolism
chemical_list: D000953:Antigens, Protozoan; D017252:CD3 Complex; D015704:CD4 Antigens; D016207:Cytokines; D053222:Fas Ligand Protein; C505658:Fasl protein, mouse; D018840:HSP70 Heat-Shock Proteins; D008562:Membrane Glycoproteins; D016693:Receptors, Antigen, T-Cell, alpha-beta; D011994:Recombinant Proteins; D019014:fas Receptor; D019286:Endopeptidase K