title: Histopathologic features seen in Gianotti-Crosti syndrome secondary to Epstein-Barr virus.
abstract: BACKGROUND
  Gianotti-Crosti syndrome (GCS) or infantile papular acrodermatitis presents as a symmetric erythematous lichenoid papular and papulovesicular eruption of the face, extremities, and buttocks, usually occurring in young children. GCS has been associated with hepatitis B and enteroviruses, as well as Epstein-Barr virus (EBV) and, rarely, cytomegalovirus.
  
  
  OBJECTIVE
  The purpose of this study was to use immunohistochemical studies to determine the pattern of the lymphoid infiltrate and evidence for viral antigens in cases of EBV-associated GCS.
  
  
  METHODS
  Routine histologic and immunohistochemical stains were evaluated in 3 patients with typical GCS. All 3 patients showed serologic evidence of an acute EBV infection. The immunohistochemical studies included monoclonal antibodies for CD3, CD4, CD8, CD20, TIA, S-100 protein, KP-1, EBV latent membrane antigen-1, and EBV-encoded nuclear antigen-2.
  
  
  RESULTS
  All biopsy specimens showed minimal epidermal spongiosis with marked papillary dermal edema. The associated inflammatory infiltrate showed a mixed mononuclear cell infiltrate with rare eosinophils. Immunohistochemical stains for latent membrane antigen-1 and EBV-encoded nuclear antigen-2 were negative for EBV. The majority of mononuclear cells showed membrane staining for CD3, 30% to 40% of the CD3 mononuclear cells showed positive staining for CD4, and 50% to 60% showed positive staining with CD8. TIA(+) cells appeared to correspond to the CD8(+) cells.
  
  
  CONCLUSIONS
  Although papillary dermal edema has been reported within the spectrum of histologic findings in GCS, it was marked and a consistent finding in the 3 cases in which EBV was the most likely etiologic agent. The presence of large numbers of cytotoxic T cells in the inflammatory infiltrate may have accentuated this histologic finding and may be a relatively distinctive histologic finding with GCS associated with EBV.
authors: Walter Reed Army Medical Center, Bethesda, Maryland, USA.
authors: K J
authors: KJ
authors: Smith
authors: H
authors: H
authors: Skelton
mesh_terms: D000169:Acrodermatitis / Q000175:diagnosis / Q000209:etiology* / Q000473:pathology*; D001707:Biopsy, Needle; D020031:Epstein-Barr Virus Infections / Q000150:complications* / Q000175:diagnosis; D019309:Epstein-Barr Virus Nuclear Antigens / Q000032:analysis*; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D012680:Sensitivity and Specificity
chemical_list: D019309:Epstein-Barr Virus Nuclear Antigens