Ethylene suppresses jasmonate-induced gene expression in nicotine biosynthesis.
In Nicotiana sylvestris, a set of nicotine biosynthesis genes were activated by exogenous application of methyl jasmonate, but the activation was effectively suppressed by simultaneous treatment with ethylene. When N. sylvestris transgenic hairy roots were treated with a natural ethylene precursor, the jasmonate-responsive expression of the promoter from a nicotine pathway enzyme gene was completely suppressed, and this suppressive effect was abolished when ethylene perception was blocked with silver cation. These and additional immunoblot results suggest that ethylene signal antagonizes jasmonate signal in nicotine biosynthesis.
Shoji
T
T
Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Japan.
Nakajima
K
K
Hashimoto
T
T
D003517:Cyclopentanes / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D005030:Ethylenes / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects* / Q000502:physiology; D009538:Nicotine / Q000096:biosynthesis*; D054883:Oxylipins; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018515:Plant Leaves; D018517:Plant Roots; D010947:Plants, Toxic; D012834:Silver / Q000494:pharmacology; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*
D003517:Cyclopentanes; D005030:Ethylenes; D054883:Oxylipins; D010937:Plant Growth Regulators; D012834:Silver; D009538:Nicotine; C011006:jasmonic acid; C036216:ethylene