Absence of CD43 fails to alter T cell development and responsiveness.
Genetic elimination of CD43 has been associated with increased T cell adhesiveness and T cell hyperresponsiveness to mitogens and alloantigens. Therefore, we investigated whether T cell development was perturbed in CD43-deficient mice by breeding CD43(null) mice with male Ag (Hy)-specific TCR-transgenic mice. Neither positive nor negative thymic selection of male Ag-specific T cells were affected by CD43 status. Furthermore, we did not observe a substantial or consistent hyperresponsive pattern in HY-CD43(null) lymph node cells compared with littermate HY-CD43(+/-) lymph node cells upon analysis of in vitro T cell stimulation with male Ag or mitogen. These observations challenged original conclusions associating absence of CD43 with T cell hyperresponsiveness and led us to re-examine this association. Reported phenotypes of CD43(null) mice have been based on mice with a mixed 129xC57BL/6 genetic background. To exclude a possible influence of genetic background differences among individual mice we analyzed CD43(null) littermates that had been back-bred onto the C57BL/6 background for seven to eight generations. We found that CD43(+) and CD43(null) littermates with the C57BL/6 background exhibited no differences in response to mitogen or alloantigen, thereby establishing that T cell hyperresponsiveness is not a general correlate of CD43 absence.
Carlow
D A
DA
The Biomedical Research Centre, University of British Columbia, Vancouver, British Columbia, Canada.
Corbel
S Y
SY
Ziltener
H J
HJ
D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis / Q000235:genetics*; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D002478:Cells, Cultured; D003433:Crosses, Genetic; D005260:Female; D006182:H-Y Antigen / Q000096:biosynthesis / Q000235:genetics; D051917:Leukosialin; D008213:Lymphocyte Activation / Q000235:genetics*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D010641:Phenotype; D011948:Receptors, Antigen, T-Cell / Q000096:biosynthesis / Q000235:genetics; D012737:Sex Factors; D012795:Sialoglycoproteins / Q000172:deficiency* / Q000235:genetics*; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology / Q000378:metabolism
D015703:Antigens, CD; D006182:H-Y Antigen; D051917:Leukosialin; D011948:Receptors, Antigen, T-Cell; D012795:Sialoglycoproteins; C497863:Spn protein, mouse