Nuclear localization of yeast Nfs1p is required for cell survival.
Saccharomyces cerevisiae Nfs1p is mainly found in the mitochondrial matrix and has been shown to participate in iron-sulfur cluster assembly. We show here that Nfs1p contains a potential nuclear localization signal, RRRPR, in its mature part. When this sequence was mutated to RRGSR, the mutant protein could not restore cell growth under chromosomal NFS1-depleted conditions. However, this mutation did not affect the function of Nfs1p in biogenesis of mitochondrial iron-sulfur proteins. The growth defect of the mutant was complemented by simultaneous expression of the mature Nfs1p, which contains the intact nuclear localization signal but lacks its mitochondrial-targeting presequence. These results suggest that a fraction of Nfs1p is localized in the nucleus and is essential for cell viability.
Nakai
Y
Y
Department of Biochemistry, Osaka Medical College, Takatsuki, Osaka 569-8686 Japan. med004@art.osaka-med.ac.jp
Nakai
M
M
Hayashi
H
H
Kagamiyama
H
H
D000595:Amino Acid Sequence; D002467:Cell Nucleus / Q000737:chemistry*; D003600:Cytosol / Q000737:chemistry; D005656:Fungal Proteins / Q000032:analysis* / Q000737:chemistry; D008928:Mitochondria / Q000737:chemistry; D024101:Mitochondrial Proteins; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins; D013466:Sulfurtransferases
D005656:Fungal Proteins; D024101:Mitochondrial Proteins; D029701:Saccharomyces cerevisiae Proteins; D013466:Sulfurtransferases; C115912:NFS1 protein, S cerevisiae