Kinesin subfamily UNC104 contains a FHA domain: boundaries and physicochemical characterization.
By sequence analysis we show that the U104 domain found in the UNC104 subfamily of kinesins is a forkhead homology-associated domain (FHA). A combination of limited proteolysis, mass spectroscopy, and physicochemical analysis define this domain as a genuine autonomously folding domain. Our data show that the FHA domain is shorter than previously reported since the C-terminal alpha-helix is not part of its minimum core. Key amino acids postulated to recognize phosphorylated residues are conserved. These data suggest that the kinesin FHA domains are functional domains involved in protein-protein interactions regulated by phosphorylation.
Westerholm-Parvinen
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Cell Biology and Cell Biophysics Program, European Molecular Biology Laboratory, Heidelberg, Germany. westerho@embl-heidelberg.de
Vernos
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Serrano
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D000595:Amino Acid Sequence; D000818:Animals; D018797:Cell Cycle Proteins; D064447:Checkpoint Kinase 2; D002942:Circular Dichroism; D016208:Databases, Factual; D051858:Forkhead Transcription Factors; D006358:Hot Temperature; D016547:Kinesins / Q000737:chemistry* / Q000235:genetics*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics; D010446:Peptide Fragments / Q000737:chemistry; D010766:Phosphorylation; D011485:Protein Binding / Q000235:genetics; D011489:Protein Denaturation; D017510:Protein Folding; D011494:Protein Kinases / Q000235:genetics; D017346:Protein Serine-Threonine Kinases; D017434:Protein Structure, Tertiary / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics; D014157:Transcription Factors / Q000235:genetics; D014508:Urea / Q000737:chemistry; D014981:Xenopus
D018797:Cell Cycle Proteins; D051858:Forkhead Transcription Factors; D009687:Nuclear Proteins; D010446:Peptide Fragments; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C418783:kinesin-like protein 4; D014508:Urea; D011494:Protein Kinases; D064447:Checkpoint Kinase 2; D017346:Protein Serine-Threonine Kinases; C067022:RAD53 protein, S cerevisiae; D016547:Kinesins