title: The bacterial flagellar cap as the rotary promoter of flagellin self-assembly.
abstract: The growth of the bacterial flagellar filament occurs at its distal end by self-assembly of flagellin transported from the cytoplasm through the narrow central channel. The cap at the growing end is essential for its growth, remaining stably attached while permitting the flagellin insertion. In order to understand the assembly mechanism, we used electron microscopy to study the structures of the cap-filament complex and isolated cap dimer. Five leg-like anchor domains of the pentameric cap flexibly adjusted their conformations to keep just one flagellin binding site open, indicating a cap rotation mechanism to promote the flagellin self-assembly. This represents one of the most dynamic movements in protein structures.
authors: Protonic NanoMachine Project, ERATO, JST, 3-4 Hikaridai, Seika, Kyoto 619-0237, Japan.
authors: K
authors: K
authors: Yonekura
authors: S
authors: S
authors: Maki
authors: D G
authors: DG
authors: Morgan
authors: D J
authors: DJ
authors: DeRosier
authors: F
authors: F
authors: Vonderviszt
authors: K
authors: K
authors: Imada
authors: K
authors: K
authors: Namba
mesh_terms: D001419:Bacteria / Q000378:metabolism / Q000648:ultrastructure*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism*; D020285:Cryoelectron Microscopy; D004058:Diffusion; D019281:Dimerization; D005407:Flagella / Q000378:metabolism* / Q000648:ultrastructure; D005408:Flagellin / Q000737:chemistry* / Q000378:metabolism*; D007091:Image Processing, Computer-Assisted; D008954:Models, Biological; D011487:Protein Conformation; D017510:Protein Folding; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary
chemical_list: D001426:Bacterial Proteins; D005408:Flagellin; C076733:FlaD protein, Bacteria