Hunting the subset-specific genes of neuroblastoma: expression profiling and differential screening of the full-length-enriched oligo-capping cDNA libraries.
BACKGROUND
Neuroblastoma (NBL) has a distinct nature in different prognostic subgroups.


METHODS
To understand the molecular mechanism of NBL's genesis and biology as well as that of the neural crest development, we constructed full-length-enriched cDNA libraries by an oligo-capping method from two different subsets of primary NBL, one with favorable biology and the other with MYCN amplification.


RESULTS
Sequencing analysis of these libraries revealed that the expression profile was markedly different between both subsets. To identify the genes differentially expressed between the subsets, semi-quantitative RT-PCR analyses are proceeding.


CONCLUSIONS
So far, 54 transcripts have been found to be expressed at high levels in favorable NBLs, and significantly at low levels in unfavorable NBLs.
Ohira
M
M
Division of Biochemistry, Chiba Cancer Center Research Institute, Chiba, Japan.
Shishikura
T
T
Kawamoto
T
T
Inuzuka
H
H
Morohashi
A
A
Takayasu
H
H
Kageyama
H
H
Takada
N
N
Takahashi
M
M
Sakiyama
S
S
Suzuki
Y
Y
Sugano
S
S
Kuma
H
H
Nozawa
I
I
Nakagawara
A
A
D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D015723:Gene Library; D006801:Humans; D008403:Mass Screening; D009447:Neuroblastoma / Q000235:genetics*; D014407:Tumor Cells, Cultured