[Use of PCR methods for detection of selected genes of Mycoplasma pneumoniae].
The aim of this study was standardization of PCR for the detection of gene encoding the P1 protein, 16S rRNA and elongation factor Tu of M. pneumoniae. A total of 13 strains of M. pneumoniae, 28 strains of other mycoplasmas and 14 strains of different bacteria causing respiratory tract infections were tested. In all of tested M. pneumoniae strains the presence of the sought genes was confirmed. The specificity of DNA was confirmed by the restriction endonuclease analysis with enzymes Hind III, Alu I and Hha I. With none of primers specific for the M. pneumoniae genes amplification of DNA from other bacteria was noted. The PCR method with the selected primers allowed to detect from 10(2) to 10(4) cfu M. pneumoniae/ml suspended in broth. The obtained results indicate that the PCR method can be used for detection of M. pneumoniae genes. A very good sensitivity and specificity predestine++ PCR as a potential quick diagnostic method for identification of M. pneumoniae in clinical specimens.
Rastawicki
W
W
Zakład Bakteriologii PZH w Warszawie.
Jagielski
M
M
Gierczyński
R
R
D004269:DNA, Bacterial / Q000302:isolation & purification*; D009177:Mycoplasma pneumoniae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D010444:Peptide Elongation Factor Tu / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards*; D012336:RNA, Ribosomal, 16S / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D012703:Serotyping / Q000379:methods; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000235:genetics; D013045:Species Specificity
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D015719:Single-Strand Specific DNA and RNA Endonucleases; D010444:Peptide Elongation Factor Tu