title: Effect of shear stress on platelet adhesion to expanded polytetrafluoroethylene, a silicone sheet, and an endothelial cell monolayer.
abstract: We visualized in real-time platelets adhering to the surface of three representative biomaterials, by using an apparatus consisting of a modified cone and plate rheometer combined with an upright epifluorescence microscope under two shear flows (0.1 and 5.0 dyne/cm2). The materials were expanded polytetrafluoroethylene (ePTFE), silicone sheet, and a monolayer of bovine endothelial cells (ECs) formed on glass, all of which are opaque materials used for artificial blood vessels and medical devices. According to quantitative analysis, the monolayer of ECs formed on glass had better blood compatibility than did either the ePTFE or the silicone sheet under shear flow conditions. Under a shear flow condition of 0.1 dyne/cm2, platelet adhesion was silicone sheet > ePTFE. In contrast, under a shear flow condition of 5.0 dyne/cm2, ePTFE > silicone sheet. These results indicate that the intensity of shear stress could modify the order of hemocompatibility of the materials. Therefore, direct observation of platelet adhesion under shear flow conditions is indispensable for testing and screening biomaterials and for providing a precise quantitative evaluation of platelet adhesion.
authors: Department of Mechanical Engineering, School of Engineering, University of Tokyo, Japan.
authors: K S
authors: KS
authors: Furukawa
authors: T
authors: T
authors: Ushida
authors: H
authors: H
authors: Sugano
authors: T
authors: T
authors: Tamaki
authors: N
authors: N
authors: Ohshima
authors: T
authors: T
authors: Tateishi
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002417:Cattle; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology*; D018056:Hemorheology; D066298:In Vitro Techniques; D008422:Materials Testing; D010973:Platelet Adhesiveness / Q000502:physiology*; D011138:Polytetrafluoroethylene; D011817:Rabbits; D012828:Silicones; D013314:Stress, Mechanical
chemical_list: D001672:Biocompatible Materials; D012828:Silicones; D011138:Polytetrafluoroethylene