title: Influence of gelatin and bovine serum lubricants on ultra-high molecular weight polyethylene wear debris generated in in vitro simulations.
abstract: Ultra-high molecular weight polyethylene (UHMWPE) wear debris induced osteolysis has a major role in the late aseptic loosening and ultimate failure of total hip replacements (THR). Clinically relevant in vitro simulations of wear are essential to predict the osteolytic potential of bearing surfaces in artificial hip joints. Newborn calf or bovine serum has been accepted as a boundary lubricant for such in vitro tests, but its biological stability has been questioned. This study compared the wear factors, number of wear particles and levels of microbial contamination produced in bovine serum and a gelatin-based lubricant. The wear factors produced by the two lubricants were not significantly different, however the wear debris morphology produced was substantially different. The bovine serum became contaminated with micro-organisms within 28 h, whereas the protein-based lubricant remained uncontaminated. The results showed that bovine serum was not a stable boundary lubricant. They also showed that although the wear factors for the two solutions were not significantly different, the protein-based lubricant was not a suitable alternative to bovine serum because the wear debris produced was not clinically relevant.
authors: Department of Mechanical Engineering, University of Leeds, UK.
authors: J
authors: J
authors: Bell
authors: A A
authors: AA
authors: Besong
authors: J L
authors: JL
authors: Tipper
authors: E
authors: E
authors: Ingham
authors: B M
authors: BM
authors: Wroblewski
authors: M H
authors: MH
authors: Stone
authors: J
authors: J
authors: Fisher
mesh_terms: D000818:Animals; D002417:Cattle; D005780:Gelatin / Q000592:standards*; D006622:Hip Prosthesis / Q000009:adverse effects*; D006801:Humans; D008153:Lubrication; D008422:Materials Testing; D010014:Osteolysis; D011095:Polyethylenes / Q000009:adverse effects*; D011237:Predictive Value of Tests; D011475:Prosthesis Failure; D012710:Serum Albumin, Bovine / Q000592:standards*; D013386:Succinates / Q000592:standards*
chemical_list: D011095:Polyethylenes; D013386:Succinates; C111601:ultra-high molecular weight polyethylene; D012710:Serum Albumin, Bovine; C060085:succinylated gelatin; D005780:Gelatin