Electron spin resonance of copper(II) as a tool for the determination of asparagine concentration in Bacillus subtilis cultures.
A procedure is presented that is based on the detection of Cu(II)-asparagine complexes by quantitative ESR, and allows in a very simple and rapid manner to evaluate the changes of asparagine concentration during the entire time range of the growth of Bacillus subtilis in a typical growth medium. The analysis is carried out in terms of the decrease of the intensity of the ESR-active mono- and di-asparagine copper(II) complexes. It is resulted that at the end of the exponential growth the asparagine concentration was reduced to values as low as 2% of the initial value. The procedure here reported may be the basis of similar methods to be used for other amino acids and prokaryote systems.
Jung
K
K
Dipartimento di Chimica, Università di Firenze, Italy.
Branciamore
S
S
Martini
G
G
D001216:Asparagine / Q000032:analysis* / Q000378:metabolism; D001412:Bacillus subtilis / Q000254:growth & development*; D003300:Copper; D003470:Culture Media / Q000032:analysis; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods
D003470:Culture Media; D001216:Asparagine; D003300:Copper