title: Up-regulation of glial cell line-derived neurotrophic factor (GDNF) following traumatic spinal cord injury.
abstract: We investigated the temporal and spatial expression patterns of the GDNF gene after subjecting rats to an acute contusion injury of the spinal cord using the weight drop technique. Reverse transcriptase-polymerase chain reaction (RT-PCR) revealed that GDNF transcription in the spinal cord began to increase within 30 min after injury and peaked within 3 h. Immunohistochemical analysis showed GDNF immunoreactivity to be present mainly in microglia and macrophages 1 day after injury, but not in neurons or astrocytes. This immediate upregulation of GDNF gene expression may be a component of an inflammatory process and probably exerts a protective effect on neurons following spinal cord injury (SCI).
authors: Department of Orthopaedic Surgery, Nagoya University School of Medicine, Japan.
authors: K
authors: K
authors: Satake
authors: Y
authors: Y
authors: Matsuyama
authors: M
authors: M
authors: Kamiya
authors: H
authors: H
authors: Kawakami
authors: H
authors: H
authors: Iwata
authors: K
authors: K
authors: Adachi
authors: K
authors: K
authors: Kiuchi
mesh_terms: D000818:Animals; D051100:Glial Cell Line-Derived Neurotrophic Factor; D007150:Immunohistochemistry; D008264:Macrophages / Q000378:metabolism; D008297:Male; D009414:Nerve Growth Factors / Q000096:biosynthesis* / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D009457:Neuroglia / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000302:isolation & purification; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013119:Spinal Cord Injuries / Q000378:metabolism*; D015854:Up-Regulation / Q000502:physiology*
chemical_list: C495041:Gdnf protein, rat; D051100:Glial Cell Line-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger