Size distribution of the urokinase mRNA decay intermediates in different tissues and cell lines.
Many genes, particularly those encoding the products participating in the regulation of transcription, replication and tissue remodeling, produce short-lived mRNA. It has been commonly accepted that once mRNA is disintegrated, the degradation process is so rapid that the decay intermediates cannot be detected. In the present study we verified this postulate and focused our attention on the quantification of the decay products of the urokinase-type plasminogen activator (uPA) mRNA that belongs to short-lived mRNAs. Using a previously described modified quantitative RT-PCR method, we have shown that intact uPA mRNA coexists in normal human tissues, Jurkat and 5637 cells with a great abundance of its degradation products. The uPA mRNA decay products were not detected in T24P cells. The content of intact uPA mRNA in normal tissues was as low as 5% of the total amount of its poly(A)(+) fraction. The size distribution of the mRNA decay products suggests that the mRNA is digested by exonucleases or/and non-specific endonuclease with cut sites evenly distributed along the mRNA chain. Different decay degrees were demonstrated for subpopulation of the uPA mRNA molecules with intact 3' and 5' ends.
Timofeeva
A V
AV
Russian Cardiology Research and Development Center, 3rd Cherepkovskaya str., 15a, Moscow 121552, Russia. angelica_t@cardio.ru
Skrypina
N A
NA
Savochkina
L P
LP
Beabealashvilli
R Sh
RSh
D015152:Blotting, Northern; D002460:Cell Line; D018076:DNA, Complementary / Q000737:chemistry; D004795:Enzyme Stability; D006801:Humans; D007668:Kidney / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D010316:Particle Size; D012333:RNA, Messenger / Q000032:analysis / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured; D014568:Urokinase-Type Plasminogen Activator / Q000737:chemistry / Q000235:genetics*
D018076:DNA, Complementary; D012333:RNA, Messenger; D014568:Urokinase-Type Plasminogen Activator