Polymerase chain reaction for definitive identification of Yersinia ruckeri.
Yersinia ruckeri causes enteric red mouth (ERM) disease in salmonids. Serologic identification of Y. ruckeri is hampered by cross-reactivity with other bacterial isolates of fish origin. Oligonucleotide primers incorporating Y. ruckeri unique sequences were designed to amplify a 409 bp fragment of Y. ruckeri 16S rDNA. The primers did not amplify other genetically related Yersinia or a wide variety of other aquatic or piscine bacteria. This assay provides a rapid, definitive identification of Y. ruckeri that is not subject to the variability inherent in serologic methods.
LeJeune
J T
JT
Department of Veterinary Microbiology and Pathology and the Washington Animal Disease Diagnostic Laboratory, Washington State University, Pullman 99164, USA.
Rurangirwa
F R
FR
D000818:Animals; D017931:DNA Primers; D004275:DNA, Ribosomal / Q000235:genetics; D005393:Fish Diseases / Q000175:diagnosis* / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012487:Salmonidae; D015007:Yersinia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D015009:Yersinia Infections / Q000175:diagnosis / Q000662:veterinary*
D017931:DNA Primers; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S