Dynamic accumulation and degradation of poly(3-hydroxyalkanoate)s in living cells of Azotobacter vinelandii UWD characterized by (13)C NMR.
The synthesis and degradation of poly(3-hydroxybutyrate) (PHB) and poly(3-hydroxybutyrate-co-hydroxyvalerate) (P(HB-co-HV)) by Azotobacter vinelandii UWD were investigated using natural abundance solution (13)C nuclear magnetic resonance (NMR) in vivo in shake flask culture and in fermenter culture. The synthesis and the degradation of poly(3-hydroxyalkanoate)s (PHA) monomers hydroxybutyrate (HB) and hydroxyvalerate (HV) had different rates. The amount of HB and HV increased dramatically in the initial degradation stage. The results suggest that the intracellular PHA of strain UWD was the subject of dynamic metabolic processing. (13)C NMR in vivo analysis provided a rapid, easy, accurate, non-destructive method to obtain valuable information on the metabolism of PHA.
Yan
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YB
Department of Biological Sciences and Biotechnology, Tsinghua University, 100084, Beijing, PR China.
Wu
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Q
Zhang
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RQ
D016948:Azotobacter vinelandii / Q000254:growth & development / Q000378:metabolism*; D002247:Carbon Isotopes; D003470:Culture Media; D006885:Hydroxybutyrates / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D011091:Polyesters / Q000378:metabolism*; D000094122:Polyhydroxybutyrates
D002247:Carbon Isotopes; D003470:Culture Media; D006885:Hydroxybutyrates; D011091:Polyesters; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates; C003182:poly-beta-hydroxybutyrate