Immunogenicity of a foreign peptide expressed within a capsid protein of an attenuated coxsackievirus.
The immunogenicity of soluble peptides can be improved by expression within recombinant microorganisms. The immunogenicity of a peptide expressed within a capsid protein of an attenuated coxsackievirus B4 was evaluated. The insertion site was chosen based on its antigenic structure. A foreign peptide was inserted into a region of the VP1 capsid protein that was identified as a T helper cell epitope. A recombinant virus containing ten amino acids of ovalbumin sequence was genetically stable and retained the biological and physical characteristics of the parental virus. The recombinant was able to elicit a T helper cell response against ovalbumin sequences. This study shows, for the first time, that coxsackievirus can be used as an expression vector and that insertion of heterologous peptides into an immunogenic region is a viable strategy for inducing T helper cell responses against foreign sequences. The implications of this work are that the attenuated coxsackievirus variant may be useful as a vaccine vector for expressing T helper cell epitopes that are important in inducing protective immunity.
Halim
S S
SS
Department of Biomedical Sciences, School of Public Health, State University of New York at Albany, Albany, NY 12237, USA.
Ostrowski
S E
SE
Lee
W T
WT
Ramsingh
A I
AI
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002213:Capsid / Q000235:genetics / Q000276:immunology*; D002460:Cell Line; D002678:Chimera / Q000235:genetics / Q000276:immunology; D004274:DNA, Recombinant / Q000235:genetics; D029822:Enterovirus B, Human / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D005822:Genetic Vectors; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010047:Ovalbumin / Q000235:genetics / Q000276:immunology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D014162:Transfection; D014779:Virus Replication
D004274:DNA, Recombinant; D011994:Recombinant Proteins; D010047:Ovalbumin