Thrombogenic potential of contrast media in an experimental model of laser-induced thrombosis.
Controversy still exists about the pro- or antithrombotic side effects of contrast media used in daily medical practice. Recent reports have shown that various contrast media, including ionic compounds, have deleterious prothrombotic actions. A new evaluation of these adverse side effects is reported here, with the study of the dose-effect relationship. Two ionic (ioxaglate and diatrizoate) and two non-ionic contrast media (iopamidol and iohexol) were studied. Experiments were done on 22 groups of 5 Wistar male rats each, using a Laser Argon-induced thrombosis model in mesenteric microvessels. Three parameters were studied: the number of laser beams needed to induce platelet thrombus formation, the number of emboli, and the duration of embolization. Platelet count and platelet aggregation also were determined. Iopamidol and iohexol induced a significant rise in both the number of emboli and the duration of embolization in mesenteric microvessels at doses up to 1 mL/kg. Ioxaglate and diatrizoate also significantly increased these parameters at doses up to 2 mL/kg. All the products tested decreased platelet count, inducing a -17 to -30% variation from control values. Diatrizoate and ioxaglate inhibited platelet aggregation, while iopamidol and Iohexol behaved as activators. All non-ionic, and to a lesser extent, all ionic contrast media demonstrated prothrombotic properties.
Aguejouf
O
O
Laboratoire d'Hématologie, Faculté de Pharmacie, Université de Bordeaux II, 146, Rue Léo Saignat, FR-33 076., Bordeaux Cedex, France.
Malfatti
E
E
Doutremepuich
F
F
Doutremepuich
C
C
D000818:Animals; D003287:Contrast Media / Q000009:adverse effects*; D003973:Diatrizoate / Q000009:adverse effects; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D007472:Iohexol / Q000494:pharmacology; D007479:Iopamidol / Q000494:pharmacology; D007485:Ioxaglic Acid / Q000009:adverse effects; D007834:Lasers / Q000009:adverse effects; D007958:Leukocyte Count; D008297:Male; D008353:Mannitol / Q000494:pharmacology; D008638:Mesenteric Arteries / Q000187:drug effects / Q000528:radiation effects; D008833:Microcirculation / Q000187:drug effects / Q000528:radiation effects; D010974:Platelet Aggregation / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D012965:Sodium Chloride / Q000494:pharmacology; D019851:Thrombophilia / Q000139:chemically induced*; D013927:Thrombosis / Q000139:chemically induced*
D003287:Contrast Media; D003973:Diatrizoate; D008353:Mannitol; D007472:Iohexol; D012965:Sodium Chloride; D007479:Iopamidol; D007485:Ioxaglic Acid