Technical advance: application of high-performance liquid chromatography with photodiode array detection to the metabolic profiling of plant isoprenoids.
The application of high-performance liquid chromatography (HPLC) using a C30 reverse-phase stationary matrix has enabled the simultaneous separation of carotenes, xanthophylls, ubiquinones, tocopherols and plastoquinones in a single chromatogram. Continuous photodiode array (PDA) detection ensured identification and quantification of compounds upon elution. Applications of the method to the characterization of transgenic and mutant tomato varieties with altered isoprenoid content, biochemical screening of Arabidopsis thaliana, and elucidation of the modes of action of bleaching herbicides are described to illustrate the versatility of the procedure.
Fraser
P D
PD
School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey TW20 0EX, UK.
Pinto
M E
ME
Holloway
D E
DE
Bramley
P M
PM
D017360:Arabidopsis / Q000737:chemistry / Q000187:drug effects / Q000235:genetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003512:Cyclohexanones / Q000494:pharmacology; D006540:Herbicides / Q000494:pharmacology; D018551:Solanum lycopersicum / Q000737:chemistry / Q000187:drug effects / Q000235:genetics; D008698:Mesylates / Q000494:pharmacology; D009154:Mutation; D010944:Plants / Q000737:chemistry* / Q000378:metabolism; D030821:Plants, Genetically Modified; D013729:Terpenes / Q000032:analysis* / Q000378:metabolism
D003512:Cyclohexanones; D006540:Herbicides; D008698:Mesylates; D013729:Terpenes; C079906:sulcotrione