Pericentromeric regions containing 1.688 satellite DNA sequences show anti-kinetochore antibody staining in prometaphase chromosomes of Drosophila melanogaster.
A striking characteristic of the centromeric heterochromatin of Drosophila melanogaster is that each chromosome carries different satellite DNA sequences. Here we show that while the major component of the 1.688 satellite DNA family expands across the centromere of the X chromosome the rest of the minor variants are located at pericentromeric positions in the large autosomes. Immunostaining of prometaphase chromosomes with the kinetocore-specific anti-BUB1 antibody reveals the transient presence of this centromeric protein in all the regions containing the 1.688 satellite.
Abad
J P
JP
Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Madrid, Spain.
Agudo
M
M
Molina
I
I
Losada
A
A
Ripoll
P
P
Villasante
A
A
D000818:Animals; D001483:Base Sequence; D002503:Centromere / Q000235:genetics / Q000378:metabolism; D002875:Chromosomes / Q000235:genetics; D016384:Consensus Sequence; D017931:DNA Primers / Q000235:genetics; D004276:DNA, Satellite / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D018386:Kinetochores / Q000378:metabolism; D008677:Metaphase / Q000235:genetics; D008969:Molecular Sequence Data
D017931:DNA Primers; D004276:DNA, Satellite