Protection against murine coxsackievirus B3 myocarditis by T cell vaccination.
T cell vaccination regulates autoimmunity by the modification of helper and suppressor T cells. The present study was performed to examine whether T cell vaccination can prevent viral myocarditis in vivo. We used coxsackievirus B3 myocarditis in mice as an animal model with the analysis of lymphokine-activated killer cell activity. Vaccination of the mice with T lymphocytes significantly prolonged survival and improved cardiac histology of murine myocarditis. The effects of T cell vaccination were most evident when T cells sensitized with the same virus were used. Vaccination of the mice with T cells from other strains of mice showed lesser protective effects. Clearance of myocardial virus was not affected by this treatment. The efficacy of T cell vaccination was confirmed in vitro by the decrease of the lymphokine-activated killer cell activity against EL-4 tumor cells and cultured myocytes. T cell vaccination of mice prolonged survival and improved myocardial lesions of animals inoculated with coxsackievirus B3.
Kishimoto
C
C
The Second Department of Internal Medicine, Faculty of Medicine, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama, 930-01, Japan. kkishi@kuhp.kyoto-u.ac.jp
Takada
H
H
Hiraoka
Y
Y
Shinohara
H
H
Kitazawa
M
M
D000818:Animals; D004770:Enterovirus / Q000378:metabolism*; D006352:Heart Ventricles / Q000473:pathology; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008811:Mice, Inbred DBA; D009205:Myocarditis / Q000821:virology; D009206:Myocardium / Q000473:pathology; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000378:metabolism* / Q000821:virology*; D006377:T-Lymphocytes, Helper-Inducer / Q000378:metabolism* / Q000821:virology*; D050378:T-Lymphocytes, Regulatory / Q000378:metabolism* / Q000821:virology*; D013997:Time Factors; D014407:Tumor Cells, Cultured; D014612:Vaccines / Q000627:therapeutic use*
D014612:Vaccines