title: The distinct disease phenotypes of the common and yellow vein strains of Tomato golden mosaic virus are determined by nucleotide differences in the 3'-terminal region of the gene encoding the movement protein.
abstract: In Nicotiana benthamiana, the common strain of the bipartite geminivirus Tomato golden mosaic virus (csTGMV) induces extensive chlorosis whereas the yellow vein strain (yvTGMV) produces veinal chlorosis on systemically infected leaves. In Datura stramonium, csTGMV produces leaf distortion and a severe chlorotic mosaic whereas yvTGMV produces only small chlorotic lesions on systemically infected leaves. Genetic recombination and site-directed mutagenesis studies using infectious clones of csTGMV and yvTGMV have identified a role in symptom production for the gene encoding the movement protein (MP). The MP amino acid at position 272, either valine (csTGMV) or isoleucine (yvTGMV), influenced symptoms in both hosts by inducing an intermediate phenotype when exchanged between the two strains. Exchange of an additional strain-specific MP amino acid at position 288, either glutamine (csTGMV) or lysine (yvTGMV), resulted in the change of symptom phenotype to that of the other strain. In situ hybridization analysis in N. benthamiana demonstrated that there was no qualitative difference in the tissue distribution of the two strains although csTGMV accumulated in higher amounts, suggesting that the efficiency of virus movement rather than distinct differences in tissue specificity of the strains is responsible for the symptom phenotypes.
authors: Department of Virus Research, John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK1.
authors: Keith
authors: K
authors: Saunders
authors: Universität Stuttgart, Biologisches Institut, Lehrstuhl für Molekularbiologie und Virologie der Pflanzen, Pfaffenwaldring 57, 70550 Stuttgart, Germany2.
authors: Christina
authors: C
authors: Wege
authors: Department of Virus Research, John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK1.
authors: Karuppannan
authors: K
authors: Veluthambi
authors: Universität Stuttgart, Biologisches Institut, Lehrstuhl für Molekularbiologie und Virologie der Pflanzen, Pfaffenwaldring 57, 70550 Stuttgart, Germany2.
authors: Holger
authors: H
authors: Jeske
authors: Department of Virus Research, John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK1.
authors: John
authors: J
authors: Stanley
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis; D013286:Datura stramonium / Q000821:virology; D017791:Geminiviridae / Q000235:genetics* / Q000472:pathogenicity; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010641:Phenotype; D010935:Plant Diseases / Q000821:virology; D053830:Plant Viral Movement Proteins; D010946:Plants, Medicinal; D010947:Plants, Toxic; D011995:Recombination, Genetic; D014026:Nicotiana / Q000821:virology; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; D004279:DNA, Viral; D053830:Plant Viral Movement Proteins; D014764:Viral Proteins