Protein production: feeding the crystallographers and NMR spectroscopists.
Protein purification efforts for structural genomics will focus on automation for the readily-expressed proteins, and process development for the more difficult ones, such as membrane proteins. Thousands of proteins are expected to be produced in the next few years. The purified proteins will be valuable reagents for the entire research community.
Edwards
A M
AM
Ontario Cancer Institute, Toronto, Canada. aled.edwards@utoronto.ca
Arrowsmith
C H
CH
Christendat
D
D
Dharamsi
A
A
Friesen
J D
JD
Greenblatt
J F
JF
Vedadi
M
M
D000818:Animals; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D015870:Gene Expression; D008565:Membrane Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D019906:Nuclear Magnetic Resonance, Biomolecular; D014176:Protein Biosynthesis; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D011786:Quality Control; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification / Q000592:standards; D012371:Robotics
D008565:Membrane Proteins; D011506:Proteins; D011993:Recombinant Fusion Proteins