title: Uterine expression of alternatively spliced mRNAs of mouse splicing factor SC35 during early pregnancy.
abstract: RNA differential display was applied to identify genes critical for the establishment of pregnancy in the mouse. One of the gene fragments identified was homologous to human SC35 splicing factor; the mouse counterpart had not then been cloned. To obtain the full cDNA sequence of the mouse gene, a cDNA library was screened and four positive clones were fully analysed. Sequencing analysis indicated that we had cloned alternatively spliced mRNA species of mouse SC35 splicing factor. A map of splicing structure for this gene's pre-mRNA was then proposed and region-specific mRNA species were tested on Northern blots. This analysis indicated that the overall expression level of SC35 mRNA was much higher in implantation sites than in inter-implantation sites in the mouse uterus during early pregnancy. The expression of alternatively spliced mRNAs for SC35 was differently regulated both during early pregnancy and by steroid hormones. Embryo-derived factors were also implicated in the up-regulation of SC35 mRNA at implantation sites. These results demonstrate, for the first time, that an essential splicing factor is regulated in a complex manner during implantation in the mouse uterus. Hence, its correct regulation could be important for the success of pregnancy.
authors: Prince Henry's Institute of Medical Research, P.O. Box 5152, Clayton, Victoria 3168, Australia. guiying.nie@med.monash.edu.au
authors: G Y
authors: GY
authors: Nie
authors: Y
authors: Y
authors: Li
authors: L
authors: L
authors: Batten
authors: B
authors: B
authors: Griffiths
authors: J
authors: J
authors: Wang
authors: J K
authors: JK
authors: Findlay
authors: L A
authors: LA
authors: Salamonsen
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern / Q000379:methods; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004958:Estradiol / Q000008:administration & dosage / Q000494:pharmacology; D004971:Estrus / Q000502:physiology; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D010052:Ovariectomy; D016133:Polymerase Chain Reaction / Q000379:methods; D011247:Pregnancy; D011374:Progesterone / Q000008:administration & dosage / Q000494:pharmacology; D012333:RNA, Messenger; D012261:Ribonucleoproteins; D000068103:Serine-Arginine Splicing Factors; D014018:Tissue Distribution; D014599:Uterus / Q000378:metabolism* / Q000473:pathology
chemical_list: D018076:DNA, Complementary; D009687:Nuclear Proteins; D012333:RNA, Messenger; D012261:Ribonucleoproteins; C499372:SRSF2 protein, mouse; C073288:SRSF2 protein, human; D000068103:Serine-Arginine Splicing Factors; D011374:Progesterone; D004958:Estradiol