Development of a high-performance liquid chromatographic-tandem mass spectrometric method for the determination of pharmacokinetics of Co 102862 in mouse, rat, monkey and dog plasma.
A method for determining concentration levels of Co 102862 in mouse, rat, monkey and dog plasma was validated in the range of 5 to 2000 ng/ml using 200 microl plasma sample volume. This validation report describes the linearity, specificity, sensitivity, reproducibility, accuracy, recovery and stability of the analytical method. The inter-day RSD ranged from 3.5 to 10.1%, intra-day RSD from 0.6 to 5.7% and intra-day accuracy (mean absolute percent difference) ranged from 2.2 to 14.9% for rat, monkey and dog plasma. A mini-validation (5-2000 ng/ml) of Co 102862 was performed in mouse plasma using the same methods. Additionally, the assay range at the low end was successfully extended to 0.5 ng/ml for monkey plasma. The method was used for the routine analysis of Co 102862 in mouse, rat, monkey and dog plasma and summary of the pharmacokinetic data are presented.
Ramu
K
K
Department of Pharmacokinetics and Drug Metabolism, CoCensys Inc., Irvine, CA 92718, USA. kumar.ramu@questpharm.com
Lam
G N
GN
Chien
B
B
D000700:Analgesics / Q000097:blood* / Q000493:pharmacokinetics; D000818:Animals; D000927:Anticonvulsants / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004285:Dogs; D000882:Haplorhini; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D015203:Reproducibility of Results; D012664:Semicarbazones / Q000097:blood* / Q000493:pharmacokinetics; D012680:Sensitivity and Specificity
D000700:Analgesics; D000927:Anticonvulsants; D012664:Semicarbazones; C119606:4-(4-fluorophenoxy)benzaldehyde semicarbazone