In vivo cytogenetics: mammalian germ cells.
This chapter summarizes the most relevant methodologies available for evaluation of cytogenetic damage induced in vivo in mammalian germ cells. Protocols are provided for the following endpoints: numerical and structural chromosome aberrations in secondary oocytes or first-cleavage zygotes, reciprocal translocations in primary spermatocytes, chromosome counting in secondary spermatocytes, numerical and structural chromosome aberrations, and sister chromatid exchanges (SCE) in spermatogonia, micronuclei in early spermatids, aneuploidy in mature sperm. The significance of each methodology is discussed. The contribution of novel molecular cytogenetic approaches to the detection of chromosome damage in rodent germ cells is also considered.
Russo
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DBSF-Department of Structural and Functional Biology, University of Insubria, Via J.H. Dunant 3, 21100, Varese, Italy. russo@civ.bio.unipd.it
D000818:Animals; D002869:Chromosome Aberrations; D005260:Female; D005854:Germ Cells / Q000473:pathology*; D008297:Male; D051379:Mice; D009152:Mutagenicity Tests / Q000379:methods*; D010053:Ovary / Q000473:pathology; D012377:Rodentia; D013094:Spermatozoa / Q000473:pathology