title: Chiral determination of amino acids by capillary electrophoresis and laser-induced fluorescence at picomolar concentrations.
abstract: In this publication we present results on the determination of enantiomers of amino acids at very low concentrations. A fluoresceine-based chiral dye was synthesized to allow the separation of diastereoisomers of D- and L-amino acids. We used capillary electrophoresis with different non-ionic surfactants (Brij). The separation parameters were optimized and separations of D- and L-isovaline, an unusual terrestrial amino acid, were obtained. The sensitivity limits were also determined using a commercial laser-induced fluorescence detector. The quantitation of these amino acids is very important to understand the process of chiral selection on Earth.
authors: SCA-CNRS, Echangeur de Solaize, Vernaison, France. odile_trambouze@post.club-internet.fr
authors: O
authors: O
authors: Vandenabeele-Trambouze
authors: M
authors: M
authors: Albert
authors: C
authors: C
authors: Bayle
authors: F
authors: F
authors: Couderc
authors: A
authors: A
authors: Commeyras
authors: D
authors: D
authors: Despois
authors: M
authors: M
authors: Dobrijevic
authors: M F
authors: MF
authors: Loustalot
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005453:Fluorescence; D007834:Lasers; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism
chemical_list: D000596:Amino Acids