title: Cellular localization of the galactose-binding lectin from human serum.
abstract: An SL2 lectin was isolated from human serum and characterized previously; cellular localization of the lectin was studied using polyclonal rabbit antibodies. According to cytofluorimetry, anti-SL2 antibodies bound only to lymphocytes and monocytes but not to other blood cells. Antibodies bound to Jurkat T cell lymphoma but did not interact with IM-9 cells of B cell origin. Moreover, the Jurkat cells bound oligosaccharides having the highest affinity to SL2 (GalNAcalpha_and Fucalpha1-2Gal), and this interaction was inhibited by anti-SL2 antibodies. Lysis of the Jurkat cells with subsequent electrophoresis and Western blotting indicates that anti-SL2 antibodies recognized a 14-kD protein.
authors: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117871, Russia. rapoport@mail.ibch.ru
authors: E M
authors: EM
authors: Rapoport
authors: M V
authors: MV
authors: Nekrasov
authors: S V
authors: SV
authors: Khaidukov
authors: E V
authors: EV
authors: Svirshchevskaya
authors: L S
authors: LS
authors: Zhigis
authors: L V
authors: LV
authors: Kozlov
authors: T N
authors: TN
authors: Batalova
authors: V P
authors: VP
authors: Zubov
authors: N V
authors: NV
authors: Bovin
mesh_terms: D000818:Animals; D006801:Humans; D019169:Jurkat Cells; D037102:Lectins / Q000097:blood* / Q000276:immunology; D011817:Rabbits; D013050:Spectrometry, Fluorescence
chemical_list: D037102:Lectins