Simultaneous estimation of phenylpropanolamine HCl, guaiphenesin and diphenylpyraline HCl in syrups by LC.
A simple, precise and accurate HPLC method was developed for the simultaneous estimation of phenyl-propanolamine HCl, guaiphenesin and diphenylpyraline HCl in syrup. The method was carried out on a Shimpak C8 column with a mobile phase consisting of acetonitrile-triethylamine (pH adjusted to 3.5 using orthophosphoric acid; 0.5%), (35:65, v/v) at a flow rate of 1.2 ml min(-1). Detection was carried out at 210 nm. Diphenhydramine was used as internal standard. The validation of the method was also carried out.
Vasudevan
M
M
Department of Pharmaceutical Analysis and Drugs Testing Laboratory, J.S.S. College of Pharmacy, Ootacamund, Tamilnadu, India. jayapalravi@hotmail.com
Ravisankar
S
S
Sathiyanarayanan
A
A
Chandan
R S
RS
D002851:Chromatography, High Pressure Liquid; D006140:Guaifenesin / Q000032:analysis*; D010665:Phenylpropanolamine / Q000032:analysis*; D010880:Piperidines / Q000032:analysis*
D010880:Piperidines; C009871:diphenylpyraline; D010665:Phenylpropanolamine; D006140:Guaifenesin