title: Multi-site DNA polymorphism analyses of Leishmania isolates define their genotypes predicting clinical epidemiology of leishmaniasis in a specific region.
abstract: Leishmania isolates from 57 cases of human cutaneous (CL), human visceral (VL), and canine visceral (CVL) leishmaniasis in Turkey were grouped by multi-site DNA polymorphism analyses into five genotypes. The initial grouping was based on DNA heterogeneity of the faster-evolving mitochondrion (kinetoplast) minicircles and the intergenic regions of two nuclear repetitive genes. Taxonomic affiliation and phylogenetic relationships of the five genotypes were inferred by comparing them with reference species for sequence heterogeneity in a approximately 1.4 kb conserved single-copy gene, encoding N-acetylglucosamine-1-phosphate transferase (NAGT). Alignment of the available sequences revealed no gap, but up to 7% scattered base substitutions, suggesting that this functionally important gene is a suitable marker. Three genotypes are completely identical to the NAGTs of the reference species, identifying them as L. infantum, L. tropica. and L. major, respectively. The remaining two are recognized as L. major NAGT variants with one and four base substitutions, respectively. As expected, Maximum Likelihood analysis of the NAGT sequences separates them into three clades, corresponding to the three species. The majority of the isolates obtained are L. infantum and L. tropica, which have been known to cause infantile VL and anthroponotic CL in western and southeastern Turkey, respectively. Unexpected is the finding of Leishmania major variants and their dispersal, possibly as previously unrecognized clinico-epidemiologic entities of CL and VL.
authors: Department of Microbiology and Immunology, University of Health Sciences, Chicago Medical School, North Chicago, Illinois, 60064 USA.
authors: L
authors: L
authors: Akman
authors: H S
authors: HS
authors: Aksu
authors: R Q
authors: RQ
authors: Wang
authors: S
authors: S
authors: Ozensoy
authors: Y
authors: Y
authors: Ozbel
authors: Z
authors: Z
authors: Alkan
authors: M A
authors: MA
authors: Ozcel
authors: G
authors: G
authors: Culha
authors: K
authors: K
authors: Ozcan
authors: S
authors: S
authors: Uzun
authors: H R
authors: HR
authors: Memisoglu
authors: K P
authors: KP
authors: Chang
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D018105:DNA, Kinetoplast / Q000032:analysis / Q000235:genetics; D016054:DNA, Protozoan / Q000032:analysis / Q000235:genetics; D004285:Dogs; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D007891:Leishmania / Q000145:classification* / Q000235:genetics*; D018314:Leishmania infantum / Q000145:classification / Q000235:genetics; D018320:Leishmania major / Q000145:classification / Q000235:genetics; D007895:Leishmania tropica / Q000145:classification / Q000235:genetics; D016773:Leishmaniasis, Cutaneous / Q000453:epidemiology / Q000469:parasitology*; D007898:Leishmaniasis, Visceral / Q000453:epidemiology / Q000469:parasitology*; D008297:Male; D008666:Metalloendopeptidases / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017855:Transferases (Other Substituted Phosphate Groups) / Q000235:genetics; D014404:Tubulin / Q000235:genetics; D014421:Turkey / Q000453:epidemiology
chemical_list: D018105:DNA, Kinetoplast; D016054:DNA, Protozoan; D014404:Tubulin; D017855:Transferases (Other Substituted Phosphate Groups); C023222:UDPacetylglucosamine-dolichyl-phosphate acetylglucosamine-1-phosphate transferase; D008666:Metalloendopeptidases; C055146:glycoprotein gp63, Leishmania