A cDNA encoding ribosomal protein RPL15 from the desiccation-tolerant bryophyte Tortula ruralis: mRNA transcripts are stably maintained in desiccated and rehydrated gametophytes.
A Tortula ruralis cDNA, Rpl15, encoding a predicted polypeptide with significant similarity to the large-subunit ribosomal protein L15 (RPL15) was isolated from a desiccated gametophyte cDNA library, and is the first L15 homologue identified from a bryophyte. The deduced 203 amino acid polypeptide is approximately 24.1 kDa, with a predicted pI of 11.1, and shares extensive identity with rat RPL15 deduced polypeptide (>57%). RNA blot hybridization using total RNA demonstrated that Rpl15 is constitutively expressed in moss gametophytes during a desiccation-rehydration cycle and that Rpl15 mRNA transcripts are maintained in desiccated gametophytes as conserved mRNAs.
Zeng
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Department of Plant Biology, Southern Illinois University-Carbondale, 62901-6509, USA.
Wood
A J
AJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D017931:DNA Primers; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics*; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014867:Water / Q000737:chemistry; D015003:Yeasts / Q000235:genetics*
D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D012269:Ribosomal Proteins; C420225:ribosomal protein RPL15, Tortula ruralis; D014867:Water