Improved sensitivity in detection of chlormequat by liquid chromatography-mass spectrometry.
Two published separations, using electrospray mass spectrometry (ES-MS), exhibit significant differences in limits of detection (LODs) for chlormequat cation in pear. Separation on ODS1, confirmed to result from ion-exchange, gives shorter analysis times and calibration over a wider concentration range than on an SCX cation-exchange column. The superior LOD using ODS1 (0.04 ng ml(-1) vs. 1.0 ng ml(-1)) results mainly from better chromatographic peak shape. Separation on ODS1 combined with optimised ES-MS detection allows direct quantification of chlormequat on an ion trap instrument at levels lower than those required for residue analysis in foods and also in drinking water.
Evans
C S
CS
Chemistry Department, University of York, Heslington, UK.
Startin
J R
JR
Goodall
D M
DM
Keely
B J
BJ
D002716:Chlormequat / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D012680:Sensitivity and Specificity
D002716:Chlormequat