Capillary electrophoresis with laser-induced fluorescence in clinical drug development routine application and future aspects.
The clinical bioanalytical setting is characterized by sample volumes of < 1 ml biological fluid (e.g. plasma, urine), a range of 3-4 decades of concentrations to be quantified and a limit of quantitation in the microg/l-ng/l range for sets of 100-5000 individual samples. Setup of capillary electrophoresis (CE) for routine application in this analytical field was successful for analytes accessible to fluorescence detection by using laser-induced fluorescence (LIF) detection. Empowerment of CE-LIF for routine serial analysis of thousands of samples includes improvement in autosampler techniques, thorough procedures for capillary treatment and particularly more advanced detection technology. Introduction of multi-capillary systems with charge-coupled device cameras and frequency doubled Ar-ion laser (lambda = 257 nm) offers this technique the chance of superiority over classical analytical assays - especially in the field of (new) low volume samples e.g. capillary blood or microdialysate encouraging clinicians to search for meaningful non-invasive samples.
Soetebeer
U B
UB
Institute of Pharmaceutical Chemistry, University of Münster, Germany.
Schierenberg
M O
MO
Möller
J G
JG
Schulz
H
H
Grünefeld
G
G
Andresen
P
P
Blaschke
G
G
Ahr
G
G
D000890:Anti-Infective Agents / Q000032:analysis; D001372:Aza Compounds; D002939:Ciprofloxacin / Q000032:analysis; D015195:Drug Design; D019075:Electrophoresis, Capillary / Q000379:methods*; D024841:Fluoroquinolones; D007834:Lasers; D000077266:Moxifloxacin; D011804:Quinolines; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*
D000890:Anti-Infective Agents; D001372:Aza Compounds; D024841:Fluoroquinolones; D011804:Quinolines; D002939:Ciprofloxacin; D000077266:Moxifloxacin