Automated, PCR-RFLP genotyping of the urokinase gene.
The urokinase-type plasminogen activator (u-PA) has been suggested to play a role in the early initiation and progression of atherosclerosis and coronary artery disease (CAD) (Grenett et aL, 1998). Recently, a common genetic polymorphism in the untranslated region of the u-PA gene was shown to be associated with syptomatic CAD. To study the possible role of this common genetic polymorphism in the u-PA gene, we have developed an automated, PCR-based assay. Automation of the PCR-RFLP genotyping of the BamHI polymorphism of the urokinase gene will support the screening of the large sample sizes required to do the population-based studies necessary to uncover disease susceptibility associations.
Sell
S M
SM
Department of Nutrition Sciences, University of Alabama at Birmingham 35294, USA. sells@uab.edu
Blasi
F
F
Booyse
F
F
D001331:Automation; D015245:Deoxyribonuclease BamHI; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D014568:Urokinase-Type Plasminogen Activator / Q000235:genetics*
D015245:Deoxyribonuclease BamHI; D014568:Urokinase-Type Plasminogen Activator