Production of capsular polysaccharide of Streptococcus pneumoniae type 14 and its purification by affinity chromatography.
We describe a rapid and efficient method for producing the capsular polysaccharide of Streptococcus pneumoniae by fermentation on tryptic soy broth and purification of this compound by using immobilized soybean lectin as an affinity adsorbent. In principle, the same strategy can be used to produce purified capsular polysaccharides from other streptococcal serotypes by selecting the appropriate lectin adsorbents.
Suárez
N
N
Laboratorio de Carbohidratos y Glicoconjugados, Departamento de Quimica Orgánica, Facultad de Química, Instituto de Higiene, C.P. 11600, Uruguay.
Fraguas
L F
LF
Texeira
E
E
Massaldi
H
H
Batista-Viera
F
F
Ferreira
F
F
D016667:Bacterial Capsules / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy; D013296:Streptococcus pneumoniae / Q000254:growth & development / Q000378:metabolism*