title: Role of hypotension in brain-death associated impairment of liver microcirculation and viability.
abstract: Hypotension in brain-dead organ donors is considered a determinant factor of graft viability. The aim of this study was to elucidate the role of hypotension in brain-death associated impairment of hepatic microcirculation and function. Male Sprague-Dawley rats with an intracranial balloon were used. Group I (n = 7) served as sham controls. In group II (n = 7) brain death was induced through inflation of an intracranial balloon. In group III (n = 7) hypotension without brain death was induced by means of pentobarbital. In group II, a steep rise of arterial pressure was followed by a fall to a lower level (P < 0.01, vs. group I). Also in group III arterial pressure was lower (P < 0.01, vs. group I). In group II, bile production was diminished (P < 0.05). Impaired sinusoidal perfusion (P < 0.01) and enhanced leukocyte endothelium interaction (P < 0.05) were documented in hepatic microvasculature. Electron microscopic analysis revealed vacuolization of hepatocytes; these changes were not observed in group III. Brain death induces specific changes of liver microcirculation, function and histomorphology. Independent of associated hypotension, brain death per se impairs donor liver graft quality.
authors.affiliation: First Department of Surgery, Japanese Red Cross Society, Wakayama Medical Center, Komatsubara 4-20, Wakayama, 640-8269, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Okamoto
authors.forename: C O
authors.initials: CO
authors.lastname: Corso
authors.forename: R
authors.initials: R
authors.lastname: Leiderer
authors.forename: W
authors.initials: W
authors.lastname: Rascher
authors.forename: Y
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Yamaoka
authors.forename: K
authors.initials: K
authors.lastname: Messmer
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001646:Bile / Q000378:metabolism; D001926:Brain Death / Q000503:physiopathology*; D006085:Graft Survival / Q000502:physiology*; D006498:Hepatectomy; D007022:Hypotension / Q000503:physiopathology*; D007511:Ischemia / Q000209:etiology*; D008099:Liver / Q000098:blood supply*; D016031:Liver Transplantation / Q000379:methods*; D008297:Male; D008833:Microcirculation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014019:Tissue Donors; D009927:Tissue and Organ Procurement
chemical_list: D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase