Electrooxidation mechanism of biogenic amines at amine oxidase modified graphite electrode.
Amine oxidase (AO, EC. 1.4.3.6) was previously shown to be a very efficient biological recognition element of amperometric biosensors for monitoring biogenic amines. The enzyme was effectively working in both mono- and bienzyme electrode designs, based on either a direct or a mediated electron-transfer pathway. This work focuses on the elucidation of the electron-transfer mechanism of the monoenzymatic unmediated AO-modified biosensor. The observed unmediated catalytic currents were assumed to be caused by (i) a direct electron-transfer process, (ii) the electrooxidation of the formed product, or (iii) their combination. Experiments supporting these assumptions are discussed in detail.
Niculescu
M
M
Department of Biotechnology, Lund University, Sweden.
Ruzgas
T
T
Nistor
C
C
Frébort
I
I
Sebela
M
M
Pec
P
P
Csöregi
E
E
D006631:Amine Oxidase (Copper-Containing) / Q000737:chemistry / Q000378:metabolism*; D015306:Biogenic Monoamines / Q000737:chemistry / Q000378:metabolism*; D002384:Catalysis; D004566:Electrodes; D010084:Oxidation-Reduction
D015306:Biogenic Monoamines; D006631:Amine Oxidase (Copper-Containing)