Direct proof for the presence and expression of two 5% different 16S rRNA genes in individual cells of Haloarcula marismortui.
A single-cell-derived pure culture of Haloarcula marismortui was obtained using the optical tweezers technique. Analysis of the 16S rRNA genes unambiguously demonstrated two 5% different 16S rRNA sequences. In addition, expression of both operons in the same cell was shown by fluorescence in situ hybridization with operon-specific probes.
Amann
G
G
Lehrstuhl für Mikrobiologie, Universität Regensburg, Germany.
Stetter
K O
KO
Llobet-Brossa
E
E
Amann
R
R
Antón
J
J
D001483:Base Sequence; D017931:DNA Primers; D019614:Haloarcula marismortui / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*
D017931:DNA Primers; D012336:RNA, Ribosomal, 16S