Adherence of Porphyromonas gingivalis to gingival epithelial cells: modulation of bacterial protein expression.
The protein profiles of Porphyromonas gingivalis (ATCC 33277 and W83) bound to KB gingival epithelial cells were analyzed by SDS-PAGE and immunoblotting. We found that a 51-kDa component was formed in bacteria that adhered to the KB cells, whereas 26- to 29-kDa bands were less intensive, in contrast to the protein profile of free bacteria. P. gingivalis ATCC 33277 incubated with protease-treated KB cells retained the profile of free bacteria. These results demonstrate the specificity of bacterial recognition of eukaryotic membrane components.
Agnani
G
G
Equipe de Biologie Buccale UPRES-EA 1256, Université de Rennes I, 2 place Pasteur, F-35000 Rennes, France.
Tricot-Doleux
S
S
Du
L
L
Bonnaure-Mallet
M
M
D018829:Adhesins, Bacterial / Q000096:biosynthesis* / Q000737:chemistry; D001422:Bacterial Adhesion; D004847:Epithelial Cells / Q000382:microbiology*; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D007624:KB Cells; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D016966:Porphyromonas gingivalis / Q000378:metabolism / Q000502:physiology*; D013379:Substrate Specificity
D018829:Adhesins, Bacterial