Urinary interleukin-6 and interleukin-8 in children with urinary tract infection.
Urinary interleukin-6 (UIL-6) and urinary interleukin-8 (UIL-8) concentrations were measured by immunoassay in 39 and 34 patients respectively, hospitalized with febrile urinary tract infection (UTI), and in 37 and 32 age-, race- and sex-matched febrile control children respectively, with negative urine cultures. UIL-6 and UIL-8 concentrations, measured in picograms per milliliter and corrected for creatinine, were compared with clinical and laboratory indicators of inflammation and bacterial virulence factors of Escherichia coli. Median UIL-6 concentrations at the time of admission were 397 pg/ml (range 0-65,789 pg/ml) in the 37 patients compared to 0 pg/ml (range 0-473.8 pg/ml) in the 37 controls (P < 0.0001). Median UIL-8 concentrations at the time of admission were 5809 pg/ml (range 0-347,368 pg/ml) in the 32 patients compared to 0 pg/ml (range 0-2231 pg/ml) in the 32 controls (P < 0.0001). UIL-6 and UIL-8 concentrations were lower (P < 0.0001 for UIL-6 and P = 0.0005 for UIL-8) in follow-up urine samples from UTI patients, obtained 48 h after the initiation of antibiotic therapy. UIL-6 and UIL-8 concentrations were statistically significantly correlated with urine white blood cells (WBC). UIL-8 concentrations were elevated in patients with E. coli organisms producing hemolysin. UIL-6 and UIL-8 are elevated in children with febrile UTI and decrease in response to antibiotic therapy. Magnitude of UIL-8 response is associated with hemolysin production, a bacterial virulence factor of E. coli. UIL-6 and UIL-8 concentrations are statistically correlated with urine WBC. UIL-6 and UIL-8 may be mediators of inflammation in children with febrile UTI.
Jantausch
B A
BA
Department of Infectious Diseases, Children's National Medical Center, George Washington University Medical Center, 111 Michigan Ave. NW, Washington, DC 20010, USA. bjantaus@cnmc.org
O'Donnell
R
R
Wiedermann
B L
BL
D002648:Child; D002675:Child, Preschool; D003404:Creatinine / Q000652:urine; D004927:Escherichia coli Infections / Q000382:microbiology / Q000652:urine; D005260:Female; D005334:Fever / Q000652:urine; D006801:Humans; D007118:Immunoassay; D007223:Infant; D007231:Infant, Newborn; D015850:Interleukin-6 / Q000652:urine*; D016209:Interleukin-8 / Q000652:urine*; D008297:Male; D012016:Reference Values; D012189:Retrospective Studies; D014552:Urinary Tract Infections / Q000382:microbiology / Q000652:urine*
D015850:Interleukin-6; D016209:Interleukin-8; D003404:Creatinine