title: [Efficiency of the extraction method with saline dextran in the pp65 antigenemia assay against human cytomegalovirus].
abstract: BACKGROUND
  To prospectively evaluate the efficacy of the saline dextran extraction method in the human cytomegalovirus (CMV) pp65 antigenemia assay (Ag-pp65).
  
  
  METHODS
  In a 2 months period we studied all the blood samples from immunodepressed patients. The extraction of leukocytes polymorphonuclear (LPMN) was obtained with a 6% dextran solution in 0.9% saline. The total leukocytes population was counted and the percentage of LPMN obtained was established. Simultaneously the LPMN were cultivated into the MRC-5 cell line by the shell vial method (SVC).
  
  
  RESULTS
  The 144 blood samples studied were divided in three groups depending of the positivity in the pp65-AG and/or in the SVC. The patients with both assays positive presented the lower overall leukocyte and LPMN counting, with a difference statistically significance with the pp65-Ag negative. No statistically difference was observed in the overall count of leukocytes in the pp65-Ag negative group. The overall percentage of LPMN obtained was 87%. The median value of pp65-Ag positive samples was 48/100,000 LPMN, this value would be 51/100,000 LPMN with the application of the real percentage of LPMN obtained (p > 0.05).
  
  
  CONCLUSIONS
  The saline dextran extraction method presented a high efficacy in the majority of blood samples studied. The immunological status and the presence of a CMV infection or disease in the patient were the principal factors that influence the extraction method. In general there is no need to adjust the pp65-Ag value obtained with this method to the real one.
authors.affiliation: Unidad de Virología, Servicio de Microbiología Clínica, Hospital Universitario Son Dureta, Palma de Mallorca. jreina@hsd.es
authors.forename: J
authors.initials: J
authors.lastname: Reina
authors.forename: F
authors.initials: F
authors.lastname: Ballesteros
authors.forename: M
authors.initials: M
authors.lastname: Galmes
authors.forename: M
authors.initials: M
authors.lastname: Mari
authors.forename: M
authors.initials: M
authors.lastname: Munar
mesh_terms: D003587:Cytomegalovirus / Q000276:immunology / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000276:immunology*; D003911:Dextrans; D015658:HIV Infections / Q000276:immunology / Q000821:virology*; D006801:Humans; D016867:Immunocompromised Host; D007958:Leukocyte Count / Q000379:methods*; D009504:Neutrophils; D010750:Phosphoproteins / Q000097:blood*; D011446:Prospective Studies; D014763:Viral Matrix Proteins / Q000097:blood*; D014773:Virology / Q000379:methods
chemical_list: D003911:Dextrans; D010750:Phosphoproteins; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa