Efficacy of chemically cross-linked antigens for acellular pertussis vaccine.
The efficacy of chemically cross-linked antigens for acellular pertussis vaccine was investigated by a murine respiratory infection model. Filamentous hemagglutinin (FHA), pertussis toxin (PT) and pertactin, prepared from a supernatant fluid of Bordetella pertussis culture or the bacterial cells, were chemically cross-linked by the addition of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysulfosuccinimide (NHSS). The biological activities of FHA and PT were inactivated by the cross-linking reaction. The cross-linked antigens induced potent protective immunity against B. pertussis more than the vaccine prepared by formalin treatment (0.2 M). These data suggest a possibility that the vaccine prepared by using EDC and NHSS is more effective than the formalin-treated vaccine.
Watanabe
M
M
Department of Microbiology and Biologics, Daiichi College of Pharmaceutical Sciences, 22-1 Tamagawa-cho, Minami-ku, 815-8511, Fukuoka, Japan. min-ind@umin.ac.jp
Nagai
M
M
Funaishi
K
K
Endoh
M
M
D018829:Adhesins, Bacterial / Q000276:immunology; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000942:Antigens, Bacterial / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D005260:Female; D006388:Hemagglutinins / Q000276:immunology; D007114:Immunization; D051379:Mice; D037342:Pertussis Toxin; D010567:Pertussis Vaccine / Q000276:immunology*; D022282:Vaccines, Acellular / Q000276:immunology; D010566:Virulence Factors, Bordetella / Q000276:immunology
D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D006388:Hemagglutinins; D010567:Pertussis Vaccine; D022282:Vaccines, Acellular; D010566:Virulence Factors, Bordetella; C065273:filamentous hemagglutinin adhesin, Bordetella pertussis; C068929:pertactin; D037342:Pertussis Toxin