title: Antibacterial effect of the culture fluid of Lentinus edodes mycelium grown in submerged liquid culture.
abstract: The antimicrobial activity of the culture fluid of Lentinus edodes mycelium grown in submerged liquid culture was tested against some common bacterial species and Candida albicans. The mycelium-free culture fluid was bacteriostatic against Streptococcus pyogenes, Staphylococcus aureus and Bacillus megaterium. The substance responsible for the activity was heat-stable, could be extracted with chloroform and had a molecular weight under 10000. These characteristics suggested that the component might be lenthionine, an antibacterial and antifungal sulphur-containing compound. The culture fluid was less toxic to human tissue culture cells than to microbes. The antibacterial activity and toxicity could not be attributed to the same component.
authors.affiliation: Zoltan Bay Foundation for Applied Research, Institute for Biotechnology, Derkovits fasor 2, Szeged, Hungary. hnora@bay.u-szeged.hu
authors.forename: N
authors.initials: N
authors.lastname: Hatvani
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001410:Bacillus megaterium / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005591:Chemical Fractionation; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D020942:Shiitake Mushrooms / Q000737:chemistry* / Q000378:metabolism; D013211:Staphylococcus aureus / Q000187:drug effects*; D013297:Streptococcus pyogenes / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media