Diagnosis of granulocytic ehrlichiosis in humans by immunofluorescence assay.
Serodiagnostic tests are widely available for tick-borne diseases. We evaluated a cell-free antigen of the human granulocytic ehrlichiosis agent. Immunofluorescence assay (IFA) with this antigen is as efficient as with the MRL kit and allows a one-step IFA with other cell-free antigens that is useful when testing sera from patients bitten by ticks.
Brouqui
P
P
Unité des rickettsies, WHO Collaborative Center for Rickettsial Reference and Research, CNRS UPRES A 6020, Faculté de Médecine, 13385 Marseille Cedex 5 France. Philippe.Brouqui@medecine.univ-mrs.fr
Salvo
E
E
Dumler
J S
JS
Raoult
D
D
D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D004536:Ehrlichia / Q000276:immunology; D016873:Ehrlichiosis / Q000097:blood / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D005455:Fluorescent Antibody Technique; D006098:Granulocytes; D018922:HL-60 Cells; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007075:Immunoglobulin M / Q000097:blood / Q000276:immunology; D011237:Predictive Value of Tests; D012698:Serologic Tests
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M