Limit of warm ischemia time before cryopreservation in rat tracheal isografts.
BACKGROUND
The viability of cadaveric tracheal grafts undergoing cryopreservation is still unclear. We evaluated the limit of warm ischemia time before cryopreservation in rat tracheal isografts.


METHODS
Each isograft was harvested from donor rats 0 to 48 hours (0, 6, 12, 18, 24, and 48 hours) after circulatory arrest, immersed in the preservative solution, and stored in a deep freezer until reaching -80 degrees C and then was kept in liquid nitrogen for 3 months. Heterotopic transplantation into the omentum was performed after the isografts were thawed. Graft morphology 3 months after transplantation was assessed.


RESULTS
The stepwise increase of warm ischemia time significantly reduced graft survival. A prolonged period of warm ischemia had a degenerative effect on both the epithelium and cartilage. The morphology of the epithelium and cartilage in isografts undergoing warm ischemia for less than 18 hours was better preserved, whereas it deteriorated in isografts undergoing warm ischemia for more than 24 hours.


CONCLUSIONS
We thus conclude that the permissible period of warm ischemia before 3-month cryopreservation to maintain tracheal isograft viability is 18 hours in rats.
Nakanishi
R
R
Second Department of Surgery, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan. ryoichi@med.uoeh-u.ac.jp
Umesue
M
M
Hashimoto
M
M
Muranaka
H
H
Hachida
M
M
Yasumoto
K
K
D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D008297:Male; D051381:Rats; D011914:Rats, Inbred BN; D013696:Temperature; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods*; D014022:Tissue Survival / Q000502:physiology*; D014132:Trachea / Q000473:pathology / Q000637:transplantation*; D014185:Transplantation, Isogeneic