Identification and characterization of gsp65, an organic hydroperoxide resistance (ohr) gene encoding a general stress protein in Enterococcus faecalis.
The Enterococcus faecalis general stress protein Gsp65 has been purified from two-dimensional gel electrophoresis. Determination of its N-terminal sequence and characterization of the corresponding gene revealed that the gsp65 product is a 133-amino-acid protein sharing homologies with organic hydroperoxide resistance (Ohr) proteins. Transcriptional analysis of gsp65 gave evidence for a monocistronic mRNA initiated 52 nucleotides upstream of the ATG start codon and for an induction in response to hydrogen peroxide, heat shock, acid pH, detergents, ethanol, sodium chloride, and tert-butylhydroperoxide (tBOOH). A gsp65 mutant showed increased sensitivity to the organic hydroperoxide tBOOH and to ethanol.
Rincé
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A
Laboratoire de Microbiologie de l'Environnement, IRBA, Université de Caen, 14032 Caen Cedex, France. rince@ibba.unicaen.ftr
Giard
J C
JC
Pichereau
V
V
Flahaut
S
S
Auffray
Y
Y
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D003434:Crossing Over, Genetic; D004352:Drug Resistance, Microbial / Q000235:genetics; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000235:genetics*; D005798:Genes, Bacterial; D006360:Heat-Shock Proteins / Q000235:genetics*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009690:Nucleic Acid Conformation; D010545:Peroxides / Q000494:pharmacology*; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
D001426:Bacterial Proteins; C422481:Gsp65 protein, Enterococcus faecalis; D006360:Heat-Shock Proteins; D010545:Peroxides; D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide