title: Genomic exploration of the hemiascomycetous yeasts: 14. Debaryomyces hansenii var. hansenii.
abstract: By analyzing 2830 random sequence tags (RSTs), totalling 2.7 Mb, we explored the genome of the marine, osmo- and halotolerant yeast, Debaryomyces hansenii. A contig 29 kb in length harbors the entire mitochondrial genome. The genes encoding Cox1, Cox2, Cox3, Cob, Atp6, Atp8, Atp9, several subunits of the NADH dehydrogenase complex 1 and 11 tRNAs were unambiguously identified. An equivalent number of putative transposable elements compared to Saccharomyces cerevisiae were detected, the majority of which are more related to higher eukaryote copia elements. BLASTX comparisons of RSTs with databases revealed at least 1119 putative open reading frames with homology to S. cerevisiae and 49 to other genomes. Specific functions, including transport of metabolites, are clearly over-represented in D. hansenii compared to S. cerevisiae, consistent with the observed difference in physiology of the two species. The sequences have been deposited with EMBL under the accession numbers AL436045-AL438874.
authors.affiliation: Collection de Levures d'Intérêt Biotechnologie, Laboratoire de Génétique Moléculaire et Cellulaire, INA-PG, INRA, UMR216, CNRS URA1925, Thiverval-Grignon, France.
authors.forename: A
authors.initials: A
authors.lastname: Lépingle
authors.forename: S
authors.initials: S
authors.lastname: Casaregola
authors.forename: C
authors.initials: C
authors.lastname: Neuvéglise
authors.forename: E
authors.initials: E
authors.lastname: Bon
authors.forename: H
authors.initials: H
authors.lastname: Nguyen
authors.forename: F
authors.initials: F
authors.lastname: Artiguenave
authors.forename: P
authors.initials: P
authors.lastname: Wincker
authors.forename: C
authors.initials: C
authors.lastname: Gaillardin
mesh_terms: D001203:Ascomycota / Q000235:genetics*; D004251:DNA Transposable Elements; D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D005656:Fungal Proteins / Q000145:classification / Q000235:genetics*; D020440:Gene Duplication; D016681:Genome, Fungal; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics; D012343:RNA, Transfer; D012441:Saccharomyces cerevisiae / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements; D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D005656:Fungal Proteins; D009687:Nuclear Proteins; D012343:RNA, Transfer