title: Role of leukocyte adhesion molecules in monocyte/ macrophage infiltration in weanling rats with unilateral ureteral obstruction.
abstract: BACKGROUND
  Unilateral ureteral obstruction (UUO) of rats is a well-established model for studying obstructive nephropathy. Meanwhile, pathophysiology of pediatric obstructive nephropathy is not well understood. In this report, we studied monocyte/macrophage infiltration and expression of intercellular adhesion molecule 1 (ICAM-1) and macrophage antigen 1 (Mac-1) in weanling rats with UUO.
  
  
  METHODS
  Three-week-old male Sprague-Dawley rats underwent left unilateral ureteral ligation. Both obstructed kidneys (OBK) and contralateral kidneys (CLK) were harvested at 3, 6, 12, 24, 72 and 120 h after surgery. Monocyte/macrophage infiltration and expression of ICAM-1 and Mac-1 were evaluated immunohistochemically, and results were compared with those of sham-operated control rats (SOK).
  
  
  RESULTS
  Monocyte/macrophage infiltration was observed in the interstitium and perivascular region in the cortex of OBK within 6 h. The CLK and SOK showed slight monocyte/macrophage infiltration. Expression of ICAM-1 was markedly observed in the periarterial and peritubular interstitium and in renal cortical peritubular capillaries 12 h after obstruction. In CLK and SOK, ICAM-1 was slightly expressed in the endothelium of microvessels and parietal linings of Bowman's capsule. Expression of Mac-1 was detected mainly in cells infiltrating the perivascular interstitium in OBK. In CLK and SOK, few Mac-1-positive cells were observed.
  
  
  CONCLUSIONS
  Adhesion molecules, ICAM-1 and Mac-1, are expected to recruit monocyte/macrophage infiltration into OBK of weanling rats with UUO.
authors.affiliation: Department of Urology, Tohoku University School of Medicine, Sendai, Japan. hfh01307@nifty.ne.jp
authors.forename: A
authors.initials: A
authors.lastname: Takeda
authors.forename: A
authors.initials: A
authors.lastname: Fukuzaki
authors.forename: H
authors.initials: H
authors.lastname: Kaneto
authors.forename: S
authors.initials: S
authors.lastname: Ishidoya
authors.forename: Y
authors.initials: Y
authors.lastname: Ogata
authors.forename: T
authors.initials: T
authors.lastname: Sasaki
authors.forename: R
authors.initials: R
authors.lastname: Konda
authors.forename: K
authors.initials: K
authors.lastname: Sakai
authors.forename: S
authors.initials: S
authors.lastname: Orikasa
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015815:Cell Adhesion Molecules / Q000502:physiology*; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology*; D016177:Macrophage-1 Antigen / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D008297:Male; D009000:Monocytes / Q000276:immunology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014517:Ureteral Obstruction / Q000276:immunology*
chemical_list: D015815:Cell Adhesion Molecules; D016177:Macrophage-1 Antigen; D018799:Intercellular Adhesion Molecule-1