title: Metabotropic glutamate receptor type 1alpha and tubulin assemble into dynamic interacting complexes.
abstract: Metabotropic glutamate receptors (mGlu receptors) are coupled to G-protein second messenger pathways and modulate glutamate neurotransmission in the brain, where they are targeted to specific synaptic locations. Very recently, we identified tubulin as an interacting partner of the mGlu(1alpha) receptor in rat brain. Using BHK-570 cells permanently expressing the receptor we have shown that this interaction occurs predominantly with soluble tubulin, following its translocation to the plasma membrane. In addition, treatment of the cells with the agonist quisqualic acid induce tubulin depolymerization and its translocation to the plasma membrane. Immunofluorescence detection of both the receptor and tubulin in agonist-treated cells reveals a disruption of the microtubule network and an increased clustering of the receptor. Collectively these data demonstrate that the mGlu(1alpha) receptor interacts with soluble tubulin and that this association can take place at the plasma membrane.
authors.affiliation: Medical Research Council, Anatomical Neuropharmacology Unit, Oxford, UK. recep@sun.bq.ub.es
authors.forename: F
authors.initials: F
authors.lastname: Ciruela
authors.forename: R A
authors.initials: RA
authors.lastname: McIlhinney
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D001710:Biotin; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D006224:Cricetinae; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D011233:Precipitin Tests; D016318:Quisqualic Acid / Q000494:pharmacology; D018094:Receptors, Metabotropic Glutamate / Q000819:agonists / Q000502:physiology*; D014404:Tubulin / Q000502:physiology*
chemical_list: D018690:Excitatory Amino Acid Agonists; D018094:Receptors, Metabotropic Glutamate; D014404:Tubulin; C104077:metabotropic glutamate receptor type 1; D001710:Biotin; D016318:Quisqualic Acid