Correlation of human CD56+ cell cytotoxicity and IFN-gamma production.
The use of an IFN-gamma ELISPOT assay to evaluate cellular immune responses has gained increasing popularity, especially as a surrogate measure for cytotoxic T lymphocyte (CTL) responses. We have compared the IFN-gamma ELISPOT assay and the traditional(51)Cr release assay for detection of human natural killer (NK) cell activity. The cell populations used for evaluation of these assays included freshly isolated and IL-2-activated peripheral blood mononuclear cells (PBMC). CD56-positive cells were demonstrated to be the primary source of the IFN-gamma signal when PBMC were evaluated with NK-sensitive targets in the IFN-gamma ELISPOT assay. IFN-gamma ELISPOT and(51)Cr release assays showed excellent correlation suggesting that NK activity can be reliably evaluated with methods other than the traditional(51)Cr release assays.
Derby
E G
EG
NCI-Frederick Cancer Research and Development Center, SAIC-Frederick, Frederick, MD 21702-1201, USA.
Reddy
V
V
Nelson
E L
EL
Kopp
W C
WC
Baseler
M W
MW
Dawson
J R
JR
Malyguine
A M
AM
D017252:CD3 Complex / Q000096:biosynthesis; D019002:CD56 Antigen / Q000096:biosynthesis*; D002460:Cell Line; D002469:Cell Separation; D002860:Chromium Radioisotopes / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D007371:Interferon-gamma / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000378:metabolism; D020014:K562 Cells; D007694:Killer Cells, Natural / Q000276:immunology* / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D015203:Reproducibility of Results; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000378:metabolism; D013997:Time Factors; D014407:Tumor Cells, Cultured
D017252:CD3 Complex; D019002:CD56 Antigen; D002860:Chromium Radioisotopes; D007376:Interleukin-2; D007371:Interferon-gamma