Impairment of Leishmania mexicana phagocytosis in peritoneal macrophages induced by Amaranthus leucocarpus lectin.
The lectin from Amaranthus leucocarpus (ALL) is specific for N-acetyl-D-galactosamine and inhibits phagocytosis of Leishmania mexicana promastigotes in Balb/c mice peritoneal macrophages by 38%. The lipophosphoglycan (LPG) purified from L. mexicana inhibits penetration of promastigotes into peritoneal macrophages by 31%; interestingly, treatment of macrophages with both, ALL and LPG, inhibits phagocytosis of promastigotes by 72%, confirming that ALL induces modification of the macrophage's phagocytic activity by a different route than mannose or C3b receptors. The Inhibitory effect of ALL was time-dependent. N-acetyl-D-galactosamine (GalNAc) or O-glycosidically linked glycoproteins modified macrophage phagocytosis of Leishmania. These results suggest that macrophage membrane glycoproteins, possessing constitutive GalNAc, can influence the signaling pathways used by this intracellular parasite to infect.
Gorocica
P
P
Departmento de Bioquímica, Instituto Nacional de Enfermedades Respiratorias, Tlalpan, México.
Hernández
P
P
Perez
A
A
Chávez
R
R
Baez
R
R
Zenteno
E
E
Lascurain
R
R
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D003208:Concanavalin A / Q000276:immunology / Q000494:pharmacology; D006023:Glycoproteins / Q000276:immunology* / Q000494:pharmacology; D006028:Glycosphingolipids / Q000276:immunology / Q000494:pharmacology; D037102:Lectins / Q000276:immunology* / Q000494:pharmacology; D007894:Leishmania mexicana / Q000276:immunology*; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010587:Phagocytosis / Q000187:drug effects / Q000276:immunology*; D037121:Plant Lectins
C121359:Amaranthus leucocarpus lectin; D006023:Glycoproteins; D006028:Glycosphingolipids; D037102:Lectins; D037121:Plant Lectins; C008290:lipophosphonoglycan; D003208:Concanavalin A