title: Genomic exploration of the hemiascomycetous yeasts: 11. Kluyveromyces lactis.
abstract: Random sequencing of the Kluyveromyces lactis genome allowed the identification of 2235-2601 open reading frames (ORFs) homologous to S. cerevisiae ORFs, 51 ORFs which were homologous to genes from other species, 64 tRNAs, the complete rDNA repeat, and a few Ty1- and Ty2-like sequences. In addition, the complete sequence of plasmid pKD1 and a large coverage of the mitochondrial genome were obtained. The global distribution into general functional categories found in Saccharomyces cerevisiae and as defined by MIPS is well conserved in K. lactis. However, detailed examination of certain subcategories revealed a small excess of genes involved in amino acid metabolism in K. lactis. The sequences are deposited at EMBL under the accession numbers AL424881-AL430960.
authors.affiliation: Laboratoire de Génétique et Microbiologie, IGM, Centre Universitaire, Orsay, France. bolotin@igmors.u-psud.fr
authors.forename: M
authors.initials: M
authors.lastname: Bolotin-Fukuhara
authors.forename: C
authors.initials: C
authors.lastname: Toffano-Nioche
authors.forename: F
authors.initials: F
authors.lastname: Artiguenave
authors.forename: G
authors.initials: G
authors.lastname: Duchateau-Nguyen
authors.forename: M
authors.initials: M
authors.lastname: Lemaire
authors.forename: R
authors.initials: R
authors.lastname: Marmeisse
authors.forename: R
authors.initials: R
authors.lastname: Montrocher
authors.forename: C
authors.initials: C
authors.lastname: Robert
authors.forename: M
authors.initials: M
authors.lastname: Termier
authors.forename: P
authors.initials: P
authors.lastname: Wincker
authors.forename: M
authors.initials: M
authors.lastname: Wésolowski-Louvel
mesh_terms: D001203:Ascomycota / Q000235:genetics; D002503:Centromere / Q000235:genetics; D015825:Chromosomes, Fungal; D004251:DNA Transposable Elements; D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D005656:Fungal Proteins / Q000235:genetics; D018628:Gene Dosage; D023061:Gene Order; D016681:Genome, Fungal; D007716:Kluyveromyces / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017386:Sequence Homology, Amino Acid
chemical_list: D004251:DNA Transposable Elements; D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D005656:Fungal Proteins; D012343:RNA, Transfer