Characterization of rhizobial isolates of Phaseolus vulgaris by staircase electrophoresis of low-molecular-weight RNA.
Low-molecular-weight (LMW) RNA molecules were analyzed to characterize rhizobial isolates that nodulate the common bean growing in Spain. Since LMW RNA profiles, determined by staircase electrophoresis, varied across the rhizobial species nodulating beans, we demonstrated that bean isolates recovered from Spanish soils presumptively could be characterized as Rhizobium etli, Rhizobium gallicum, Rhizobium giardinii, Rhizobium leguminosarum bv. viciae and bv. trifolii, and Sinorhizobium fredii.
Velázquez
E
E
Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain. evp@gugu.usal.es
Martínez-Romero
E
E
Rodríguez-Navarro
D N
DN
Trujillo
M E
ME
Daza
A
A
Mateos
P F
PF
Martínez-Molina
E
E
van Berkum
P
P
D004586:Electrophoresis / Q000379:methods; D007887:Fabaceae / Q000382:microbiology*; D010946:Plants, Medicinal; D012329:RNA, Bacterial / Q000032:analysis; D012342:RNA, Small Nuclear / Q000032:analysis*; D012231:Rhizobium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D020579:Sinorhizobium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
D012329:RNA, Bacterial; D012342:RNA, Small Nuclear