Mating in Bacillus thuringiensis can induce plasmid integrative prophage J7W-1.
Bacillus thuringiensis serovar israelensis, a bacterium which possesses plasmid transfer ability after mating, has been lysogenized by plasmid integrative phage J7W-1. The induction of phage in this J7W-1 lysogen was observed after mating with phage-insensitive strains, such as B. thuringiensis serovar thuringiensis, B. cereus and B. subtilis, as well as the phage-sensitive strain serovar israelensis. The phage induction was not observed after mating with B. thuringiensis strains AF101, serovar dendrolimus and serovar indiana. Because these strains are naturally associated with J7W-1 or its related phage, the data strongly suggest a constitutive expression of the repressor encoded by the prophage in these strains. However, the phage induction was observed in B. thuringiensis serovar aizawai, although it contained the J7W-1 DNA homologous region(s).
Kanda
K
K
Institute of Applied Microbiology, Department of Applied Biological Sciences, Faculty of Agriculture, Saga University, Saga 840-8502, Japan. kandak@cc.saga-u.ac.jp
Takada
Y
Y
Kawasaki
F
F
Kato
F
F
Murata
A
A
D017103:Bacillus Phages / Q000254:growth & development*; D001413:Bacillus thuringiensis / Q000502:physiology* / Q000821:virology; D013997:Time Factors; D010948:Viral Plaque Assay; D014775:Virus Activation