Involvement of multiple genetic loci in Staphylococcus aureus teicoplanin resistance.
Teicoplanin resistance was transformed from a teicoplanin-resistant Staphylococcus aureus into the susceptible strain BB255 to give strain BB938. The cell wall composition, amidation of the iD-glutamate, and peptide crosslinking were identical in BB938 as in BB255 except for a 60% increased length of the glycan chain. Transductional crosses revealed that at least two distinct loci contributed in a cumulative fashion to teicoplanin resistance. One of these loci correlated with a mutation inactivating the anti-sigma factor RsbW. This mutation must have occurred during transformation and selection for teicoplanin resistance in BB938. Genetic manipulations involving the sigB operon showed that transcription factor SigB contributed to decreased teicoplanin susceptibility.
Bischoff
M
M
Institute of Medical Microbiology, University of Zürich, Gloriastr. 32, Postfach, CH-8028 Zürich, Switzerland.
Roos
M
M
Putnik
J
J
Wada
A
A
Glanzmann
P
P
Giachino
P
P
Vaudaux
P
P
Berger-Bächi
B
B
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D002473:Cell Wall / Q000737:chemistry; D002874:Chromosome Mapping; D004269:DNA, Bacterial; D004352:Drug Resistance, Microbial / Q000235:genetics; D018432:Drug Resistance, Multiple / Q000235:genetics; D006801:Humans; D008826:Microbial Sensitivity Tests; D009876:Operon / Q000235:genetics; D010457:Peptidoglycan / Q000032:analysis; D010860:Pigments, Biological / Q000378:metabolism; D051381:Rats; D012808:Sigma Factor / Q000235:genetics / Q000378:metabolism; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics*; D017334:Teicoplanin / Q000494:pharmacology*; D014161:Transduction, Genetic; D014169:Transformation, Bacterial
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D010457:Peptidoglycan; D010860:Pigments, Biological; C079011:SigB protein, Bacteria; D012808:Sigma Factor; D017334:Teicoplanin