title: Kinetic and thermodynamic analysis of leech-derived tryptase inhibitor interaction with bovine tryptase and bovine trypsin.
abstract: The interaction of leech-derived tryptase inhibitor (LDTI) with bovine liver capsule tryptase (BLCT) and bovine trypsin has been studied using both thermodynamic and kinetic approaches. Several differences were detected: (i) the equilibrium affinity of LDTI for BLCT (Ka = 8.9 x 10(5) M(-1)) is about 600-fold lower than that for bovine trypsin (Ka = 5.1 x 10(8) M(-1)); (ii) LDTI behaves as a purely non-competitive inhibitor of BLCT, while it is a purely competitive inhibitor of bovine trypsin. These functional data are compared with those previously reported for the LDTI binding to human tryptase, where tight inhibition occurs at two of the four active sites of the tetramer (Ka = 7.1 x 10(8) M(-1)). Amino acid sequence alignment of BLCT, human betaII-tryptase and bovine trypsin allows us to infer some possible structural basis for the observed functional differences.
authors.affiliation: Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Università Tor Vergata, Roma, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Erba
authors.forename: L
authors.initials: L
authors.lastname: Fiorucci
authors.forename: C P
authors.initials: CP
authors.lastname: Sommerhoff
authors.forename: M
authors.initials: M
authors.lastname: Coletta
authors.forename: F
authors.initials: F
authors.lastname: Ascoli
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D006801:Humans; D007700:Kinetics; D007865:Leeches; D008969:Molecular Sequence Data; D011506:Proteins / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases / Q000378:metabolism*; D015842:Serine Proteinase Inhibitors / Q000378:metabolism*; D013816:Thermodynamics; D014357:Trypsin / Q000378:metabolism*; D053802:Tryptases
chemical_list: D011506:Proteins; D015842:Serine Proteinase Inhibitors; C090901:leech-derived tryptase inhibitor C; D012697:Serine Endopeptidases; D014357:Trypsin; D053802:Tryptases