[Negative effect of the components of the lysis-centrifugation system in the growth of mycobacteria in MGIT and Septi-Chek AFB liquid media].
BACKGROUND
The lysis-centrifugation system (Isolator system) is a technique with excellent results in the recovery of mycobacteria from blood specimens. This system consists mainly of saponin (SAP), polypropylenglycol (PPG), and sodium polianthol sulfonate (SPS). The objective of this work was to determine the effect of SAP, PPG, and SPS on the growth of Mycobacterium avium, M. kansasii, M. tuberculosis, and M. xenopi in fluid culture media MGIT and Septi-Chek AFB.


METHODS
Two concentrations each of SAP, PPG, and SPS were prepared, and were added in 0.1 ml amounts (alone, in pairs and in combination) to fluid media MGIT and Septi-Chek AFB. Fluid culture media were then in individually inoculated with two different concentrations (10(3) and 10(5) CFU/ml) of each of the four mycobacterial strains used in this study. Culture media were incubated at 37 degrees C and were checked for growth daily.


RESULTS
SAP, PPG, and SPS did not inhibit growth of mycobacteria but growth of these strains was indeed retarded (a lengthier time was required for detection of bacterial growth compared with the positive control). Final concentrations of SAP, PPG, and SPS which retarded mycobacterial growth varied, depending upon species, mycobacterial inoculum size, and fluid culture media used.


CONCLUSIONS
Components included in the lysy-centrifugation system (SAP, PPG, and SPS), either alone or in combination retarded growth of M. avium, M. kansasii, M. tuberculosis, and M. xenopi in 10(3) and 10(5) CFU/ml concentrations in fluid culture media MGIT and Septi-Chek AFB. These results suggest that strategies should be adopted to decrease the concentrations of these three components, present in the sediment of the processed blood by the Isolator System, which eventually are going to be added to fluid media MGIT and Septi-Check AFB.
Gamboa
F
F
Centro de Investigaciones Odontológicas, Facultad de Odontología, Pontificia Universidad Javeriana, Carrera 7 No. 40-62, Bogotá, Colombia. gamboa@javercol.javeriana.edu.co
de la Rosa
Z
Z
Bustillo
J
J
Mora
M P
MP
Hernández
I
I
Mirque
F
F
D001431:Bacteriological Techniques / Q000379:methods*; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D009161:Mycobacterium / Q000187:drug effects* / Q000254:growth & development; D009162:Mycobacterium avium / Q000187:drug effects / Q000254:growth & development; D019909:Mycobacterium kansasii / Q000187:drug effects / Q000254:growth & development; D019912:Mycobacterium xenopi / Q000187:drug effects / Q000254:growth & development; D009170:Nontuberculous Mycobacteria / Q000254:growth & development; D011074:Polyanetholesulfonate / Q000494:pharmacology*; D019946:Propylene Glycol / Q000494:pharmacology*; D012503:Saponins / Q000494:pharmacology*
D003470:Culture Media; D012503:Saponins; D011074:Polyanetholesulfonate; D019946:Propylene Glycol