title: [Use of method of protein engineering in studying calcium-binding proteins].
abstract: Major results of the use of protein engineering methods in studies of calcium-binding proteins with the highest affinity for calcium and known three-dimensional structure (parvalbumin, calmodulin, troponin C, calbindin, recoverin, alpha-lactalbumin, and others) are presented. Specific features of recombinant calcium-binding proteins are discussed. Experiments with genetic introduction of fluorescent probes, tryptophan and tyrosine, into proteins are overviewed. Effects of mutations in different parts of protein molecules (calcium-binding loops, hydrophobic core, and others) on their structure and properties and attempts of creation of artificial calcium-binding sites are discussed.
authors.affiliation: Institute of Biological Instrumentation, Russian Academy of Sciences, Pushchino, Moscow Region, 142290 Russia.
authors.forename: S E
authors.initials: SE
authors.lastname: Permiakov
authors.forename: E A
authors.initials: EA
authors.lastname: Permiakov
mesh_terms: D002135:Calcium-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D015202:Protein Engineering
chemical_list: D002135:Calcium-Binding Proteins