Development of monoclonal antibodies which specifically recognize Entamoeba histolytica in preserved stool samples.
We report the generation of monoclonal antibodies against a recombinant 170-kDa subunit of the Gal or GalNAc lectin of Entamoeba histolytica that specifically recognize E. histolytica but not Entamoeba dispar in preserved stool samples. These antibodies do not cross-react with other bowel protozoa, including Entamoeba coli, Giardia lamblia, and Dientamoeba fragilis.
Yau
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YC
Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Ontario, Canada M5S 1A8.
Crandall
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Kain
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KC
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000953:Antigens, Protozoan / Q000276:immunology; D003429:Cross Reactions; D004029:Dientamoeba / Q000276:immunology; D004747:Entamoeba / Q000276:immunology; D004748:Entamoeba histolytica / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*; D004749:Entamoebiasis / Q000175:diagnosis*; D005243:Feces / Q000469:parasitology; D016829:Giardia lamblia / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D011994:Recombinant Proteins