Preventive effect of several antioxidants after oxidative stress on rat brain homogenates.
Brain homogenate was used as a model system to study antioxidant properties of several natural and synthetic antioxidants under oxidative stress. Oxidative stress was induced by Fe/ascorbate system and lipid peroxidation as well as protein modification were studied. Thiobarbituric acid reactive substances (TBARS) were used as a marker of lipid peroxidation. The preventive effect concerning lipid peroxidation decreased in the order: buthylated hydroxytoluene (BHT) (3.5), stobadine (ST) (35), serotonin (54), trolox (98), U 74389G (160), melatonin (3100), (the numbers in the brackets represent IC50 in micromol/l). Methylprednisolone had no effect, and spin traps interfered with TBARS determination. Concerning creatine kinase (CK) activity as a selected marker of oxidative modification of proteins, the preventive effect of antioxidants (30 micromol/l) decreased in the order: BHT (30), trolox (75), stobadine (ST) (77), alpha-phenyl-N-tert-buthylnitrone (PBN) (87), sodium salt of N-tert-buthyl-C-(phenyl-2-sulfone) nitrone (SPBN) (90), (the numbers in the brackets represent the loss of CK activity in percentages, when 100% was the loss of CK activity in the absence of any antioxidant). The nonglucocorticoid steroid U 74389G, methylprednisolone and serotonin had no preventive effects, while melatonin had antioxidant effect only in a higher concentration (1 mmol/l).
Horáková
L
L
Institute of Experimental Pharmacology, Slovak Academy of Sciences, Bratislava. exfahorl@savba.sk
Ondrejicková
O
O
Bachratá
K
K
Vajdová
M
M
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001557:Benzenesulfonates / Q000494:pharmacology; D001921:Brain / Q000187:drug effects*; D001930:Brain Injuries / Q000517:prevention & control*; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D002243:Carbolines / Q000494:pharmacology; D002839:Chromans / Q000494:pharmacology; D003402:Creatine Kinase / Q000378:metabolism; D003497:Cyclic N-Oxides; D020128:Inhibitory Concentration 50; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008550:Melatonin / Q000494:pharmacology; D008775:Methylprednisolone / Q000494:pharmacology; D008956:Models, Chemical; D018696:Neuroprotective Agents / Q000494:pharmacology; D009589:Nitrogen Oxides / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects*; D010100:Oxygen / Q000378:metabolism; D011281:Pregnatrienes / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000494:pharmacology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000975:Antioxidants; D001557:Benzenesulfonates; D002243:Carbolines; D002839:Chromans; D003497:Cyclic N-Oxides; D018696:Neuroprotective Agents; D009589:Nitrogen Oxides; D011281:Pregnatrienes; D017392:Thiobarbituric Acid Reactive Substances; C074965:U 74389F; C094272:N-tert-butyl-(2-sulfophenyl)nitrone; D002084:Butylated Hydroxytoluene; D012701:Serotonin; C029217:phenyl-N-tert-butylnitrone; D003402:Creatine Kinase; D008550:Melatonin; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid; D010100:Oxygen; D008775:Methylprednisolone; C100106:dicarbine