Effect of triterpenoids on the inflammation induced by protein kinase C activators, neuronally acting irritants and other agents.
In order to establish the mode of the anti-inflammatory activity of triterpenoids, 11 naturally occurring compounds were assayed on mouse ear oedema induced by the protein kinase C activators, mezerein, 12-O-tetradecanoylphorbol-13-acetate (TPA), two 12-deoxyphorbol-13-monoesters (13-tetradecanoate (DPT) and 13-phenylacetate (DPP)) and bryostatin 1, and by resiniferatoxin, xylene and arachidonic acid. The effects on bradykinin-induced paw oedema and on the rat skin inflammation caused by hydrogen peroxide were also examined. The oedema induced by mezerein and DPT was reduced to different extents by the triterpenoids administered epicutaneously (0.5 mg per ear). Against DPT-induced oedema, lupane and oleanane derivatives were the most effective compounds. Oleananes and lupanes possessing a carboxyl group were active against bryostatin 1-induced oedema. Most of the triterpenoids were ineffective against the neurogenic inflammation caused by resiniferatoxin and xylene. Many triterpenoids, especially oleanane and lupane alcoholic derivatives, were active against the plantar oedema induced by bradykinin and on the intradermal inflammation induced by hydrogen peroxide. In conclusion, the anti-inflammatory activity of triterpenoids may depend on inhibition of protein kinase C, without any involvement of neurogenic inflammatory mechanisms.
Huguet
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Departament de Farmacologia, Facultat de Farmàcia, Universitat de València, Avda. Vicent Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain.
del Carmen Recio
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Máñez
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Giner
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Ríos
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D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D016718:Arachidonic Acid / Q000494:pharmacology; D001920:Bradykinin / Q000494:pharmacology; D017453:Dermatitis, Irritant / Q000201:enzymology / Q000209:etiology / Q000517:prevention & control*; D004224:Diterpenes; D004423:Ear; D004487:Edema / Q000139:chemically induced / Q000201:enzymology / Q000517:prevention & control*; D020536:Enzyme Activators / Q000633:toxicity*; D005260:Female; D005949:Glucose Oxidase / Q000378:metabolism; D007509:Irritants / Q000494:pharmacology; D051379:Mice; D015394:Molecular Structure; D020078:Neurogenic Inflammation / Q000139:chemically induced / Q000201:enzymology / Q000517:prevention & control*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species; D012867:Skin / Q000378:metabolism; D013729:Terpenes / Q000494:pharmacology; D013997:Time Factors; D014315:Triterpenes / Q000494:pharmacology*
D000893:Anti-Inflammatory Agents; D004224:Diterpenes; D020536:Enzyme Activators; D007509:Irritants; D017382:Reactive Oxygen Species; D013729:Terpenes; D014315:Triterpenes; D016718:Arachidonic Acid; C011309:mezerein; D005949:Glucose Oxidase; D011493:Protein Kinase C; D001920:Bradykinin