Comparative genomic hybridization of medulloblastomas and clinical relevance: eleven new cases and a review of the literature.
Medulloblastomas are highly malignant primitive neuroectodermal tumors of the cerebellum that display a wide variety of histopathological patterns. However, these patterns do not provide an accurate prediction of clinical-biological behavior and no satisfactory morphological grading system has ever been presented. Genetic alterations may provide additional diagnostic information and allow clinically relevant subgrouping of primitive neuroectodermal tumors. We examined 10 medulloblastomas and one medulloblastoma cell line. One amplification site on chromosome 8q24 was detected in the cell line corresponding to the known amplification of the c-myc gene in this cell line. The gain of 2p21-24 in two tumors was shown to represent amplification of the N-myc gene by Southern blot hybridization and fluorescence in situ hybridization. The data show that the isochromosome 17 can be recognized using comparative genomic hybridization (CGH) by the typical combination of loss of 17p combined with gain of 17q. No specific pattern of genetic alterations could be linked to the clinical behavior of the tumors. We have compared our results with previous CGH studies on medulloblastomas.
Gilhuis
H J
HJ
Department of Neurology, University Medical Center St Radboud, PO Box 9101, 6500 HB, Nijmegen, The Netherlands. h.gilhuis@czzoneu.azn.nl
Anderl
K L
KL
Boerman
R H
RH
Jeuken
J M
JM
James
C D
CD
Raffel
C
C
Scheithauer
B W
BW
Jenkins
R B
RB
D000293:Adolescent; D000328:Adult; D015139:Blotting, Southern; D002528:Cerebellar Neoplasms / Q000235:genetics* / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D002886:Chromosomes, Human, Pair 17; D002898:Chromosomes, Human, Pair 8; D015342:DNA Probes; D018450:Disease Progression; D005260:Female; D005784:Gene Amplification / Q000235:genetics*; D016259:Genes, myc / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007223:Infant; D008297:Male; D008527:Medulloblastoma / Q000235:genetics* / Q000473:pathology; D014407:Tumor Cells, Cultured
D015342:DNA Probes