Totally synthetic polymer with lectin-like function: induction of killer cells by the copolymer of 3-acrylamidophenylboronic acid with N,N-dimethylacrylamide.
Lectin-like function was demonstrated in this study for a novel water-soluble polymer with phenylboronic acid residues (poly (AAPBA-DMAm)), which induced appreciable proliferation of murine spleen lymphocytes with an increased expression of interleukin-2 (IL-2) receptor on their surface. Consequently, boosted proliferation of lymphocytes with cytotoxic action to YAC-1 cells was achieved by concurrent addition of IL-2 with poly(AAPBA-DMAm) in the medium, indicating this boronate-containing polymer to be worked as an effective immuno-adjuvant for the induction of lymphokine-activated killer (LAK) cells. Flow-cytofluorimetry study revealed that poly(AAPBA-DMAm) competitively inhibited the cellular binding of N-acetylneuraminic acid-specific lectin (Limax Flavus Agglutinin) in a concentration-dependent manner, suggesting that phenylboronate moiety in the polymer may recognize N-acetylneur- aminic acid (sialic acid) residues existing on the plasma-membrane surface of lymphocytes to induce their proliferation.
Uchimura
E
E
Department of Materials Science, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Otsuka
H
H
Okano
T
T
Sakurai
Y
Y
Kataoka
K
K
D000178:Acrylamides / Q000737:chemistry*; D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D001667:Binding, Competitive; D001897:Boronic Acids / Q000737:chemistry*; D002455:Cell Division / Q000187:drug effects; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D005434:Flow Cytometry; D005470:Fluorometry; D007376:Interleukin-2 / Q000494:pharmacology; D015979:Killer Cells, Lymphokine-Activated / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D037102:Lectins / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D051379:Mice; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology*; D015375:Receptors, Interleukin-2 / Q000378:metabolism; D013154:Spleen / Q000276:immunology
C419826:3-acrylamidophenylboronic acid; D000178:Acrylamides; D000276:Adjuvants, Immunologic; D001897:Boronic Acids; D007376:Interleukin-2; D037102:Lectins; D011108:Polymers; D015375:Receptors, Interleukin-2; C099046:N,N-dimethylacrylamide; D019158:N-Acetylneuraminic Acid