title: Association of bovine DRB3 alleles with immune response to FMDV peptides and protection against viral challenge.
abstract: We have analysed the influence of bovine MHC (BoLA) polymorphism on the immune response and degree of protection induced by peptide vaccines against foot-and-mouth disease (FMD) in cattle. The peptides used for animal immunisation were: A (VP1(138-156)), AT (peptide A linked to VP1(21-40)) and ACT (peptide A, linked to VP1(196-209) and VP1(21-40)). Sixteen different DRB3 types were found among the 46 cattle analysed by PCR-RFLP typing. No absolute correlation was observed, for any type, with the serum neutralising titres (SNT) values and the protection induced. However, among the most common haplotypes present, associations were observed between expression of different types with the levels of SNT and/or protection induced by peptides A and ACT. Thus, types DRB3.2*1, 3 and 7 were associated with increased levels of protection. In contrast, types DRB3.2*12 and 18 were associated non-protection, and DRB3.2*12 was also associated with low SNT titres. Overall, the results indicate that the polymorphism in BoLA class II molecules affects both the immune response and protection induced by potential FMD peptide vaccines.
authors.affiliation: CISA-INIA, Valdeolmos 28130, Madrid, Spain.
authors.forename: M M
authors.initials: MM
authors.lastname: García-Briones
authors.forename: G C
authors.initials: GC
authors.lastname: Russell
authors.forename: R A
authors.initials: RA
authors.lastname: Oliver
authors.forename: C
authors.initials: C
authors.lastname: Tami
authors.forename: O
authors.initials: O
authors.lastname: Taboga
authors.forename: E
authors.initials: E
authors.lastname: Carrillo
authors.forename: E L
authors.initials: EL
authors.lastname: Palma
authors.forename: F
authors.initials: F
authors.lastname: Sobrino
authors.forename: E J
authors.initials: EJ
authors.lastname: Glass
mesh_terms: D000483:Alleles; D000818:Animals; D005537:Aphthovirus / Q000276:immunology*; D002213:Capsid / Q000276:immunology*; D036022:Capsid Proteins; D002417:Cattle; D006683:HLA-DQ Antigens / Q000235:genetics; D006684:HLA-DR Antigens / Q000235:genetics* / Q000276:immunology; D059826:HLA-DRB3 Chains; D010446:Peptide Fragments / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D036022:Capsid Proteins; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D059826:HLA-DRB3 Chains; D010446:Peptide Fragments; C442501:VP1 protein, Foot-and-mouth disease virus; D014765:Viral Vaccines