title: Impact of Ig-Therasorb immunoapheresis on stability of xenogeneic ex vivo porcine liver perfusion--value of aminotransferases and flow rates for the assessment of metabolic graft viability.
abstract: Due to growing shortage of donor organs, the concept of extracorporeal pig liver perfusion in the treatment of acute liver failure has been rediscovered. Immunomodulation, such as immunoapheresis or inhibition of complement, results in long-term perfusion without exact knowledge of the remaining metabolic graft viability. This study was aimed at the comparison of conventional parameters of graft stability such as perfusion rates and release of aminotransferases with parameters of metabolic graft function. Ig-Therasorb immunoapheresis (IA) of the xenogeneic perfusate was performed to protect the discordant pig livers from hyperacute rejection, mediated by preformed naturally occurring human xenogeneic antibodies. The application of IA created stable autologous graft reperfusion after a short time of xenoperfusion, but it was not able to prevent the livers from severe synthetic and functional damage. In the future, improvement of xenogeneic graft function, rather than pure prolongation of perfusion, must be the principal aim.
authors.affiliation: Department of General and Visceral Surgery, Friedrich-Schiller-University of Jena, Germany. Kornberg@bach.med.uni-jena.de
authors.forename: A
authors.initials: A
authors.lastname: Kornberg
authors.forename: O
authors.initials: O
authors.lastname: Dietz
authors.forename: H
authors.initials: H
authors.lastname: Mau
authors.forename: J
authors.initials: J
authors.lastname: Scheele
authors.forename: C
authors.initials: C
authors.lastname: Hammer
mesh_terms: D000818:Animals; D001781:Blood Component Removal / Q000379:methods*; D006439:Hemodynamics; D007136:Immunoglobulins / Q000627:therapeutic use*; D008099:Liver / Q000201:enzymology; D006435:Renal Dialysis / Q000379:methods*; D013552:Swine; D000637:Transaminases / Q000378:metabolism*; D014183:Transplantation, Heterologous
chemical_list: D007136:Immunoglobulins; D000637:Transaminases