Chip-based optical detection of DNA hybridization by means of nanobead labeling.
A new scheme for the detection of molecular interactions based on optical readout of nanoparticle labels has been developed. Capture DNA probes were arrayed on a glass chip and incubated with nanoparticle-labeled target DNA probes, containing a complementary sequence. Binding events were monitored by optical means, using reflected and transmitted light for the detection of surface-bound nanoparticles. Control experiments exclude significant influence of nonspecific binding on the observed contrast. Scanning force microscopy revealed the distribution of nanoparticles on the chip surface.
Reichert
J
J
Institute of Physical High Technology, Jena, Germany.
Csáki
A
A
Köhler
J M
JM
Fritzsche
W
W
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D015342:DNA Probes; D009693:Nucleic Acid Hybridization; D055095:Optics and Photonics
D015342:DNA Probes; D004247:DNA