Rapid identification of minor histocompatibility antigen HA-1 subtypes H and R using fluorescence-labeled oligonucleotides.
Donor-recipient disparitiy of the minor histocompatibility antigen HA-1 is relevant for the development of graft-versus-host disease after HLA-matched sibling allogeneic bone marrow transplantation in HLA-A*0201-positive individuals. Two different alleles of HA-1 with a single amino acid polymorphism have been identified. Here we describe a time- and cost-efficient method for HA-1 typing of genomic DNA, using site-specific hybridization probes with the LightCycler. This method was compared with standard techniques as sequencing or allele-specific polymerase chain reaction (PCR) and proved to be specific, reliable and reproducible. We conclude that HA-1-subtyping using fluorescent-labeled oligonucleotides represents a attractive method for the screening of samples before allogeneic transplantation in HLA-A*0201-positive individuals.
Kreiter
S
S
III, Medical Department of Medicine, Hematology, Oncology and Pulmonology, Johannes Gutenberg-University, Mainz, Germany.
Wehler
T
T
Landt
O
O
Huber
C
C
Derigs
H G
HG
Hess
G
G
D005456:Fluorescent Dyes; D006801:Humans; D015778:Minor Histocompatibility Antigens / Q000145:classification / Q000235:genetics*; D009841:Oligonucleotides; D009842:Oligopeptides / Q000145:classification / Q000235:genetics*; D013997:Time Factors
D005456:Fluorescent Dyes; C110615:HA-1 antigen; D015778:Minor Histocompatibility Antigens; D009841:Oligonucleotides; D009842:Oligopeptides