Evaluation of nonisotopic DNA hybridization methods for detection of the tdh gene of vibrio parahaemolyticus.
Production of the thermostable direct hemolysin (TDH) by Vibrio parahaemolyticus is associated with pathogenicity of the organism and is encoded by the tdh gene. The timely resolution of seafood-associated outbreaks requires rapid and accurate detection of pathogenic V. parahaemolyticus. The specificity of alkaline phosphatase- and digoxigenin-labeled tdh gene probes was evaluated against 61 strains of V. parahaemolyticus (including isolates from recent outbreaks involving oysters from the Pacific Northwest, Texas, and New York), 85 strains of other vibrios, and 7 strains of non-vibrio species from clinical and environmental sources. The probes were specific for detection of the V. parahaemolyticus tdh gene.
McCarthy
S A
SA
Gulf Coast Seafood Laboratory, US Food and Drug Administration, Dauphin Island, Alabama 36528, USA. smccarth@cfsan.fda.gov
DePaola
A
A
Kaysner
C A
CA
Hill
W E
WE
Cook
D W
DW
D000469:Alkaline Phosphatase; D000818:Animals; D001427:Bacterial Toxins; D001483:Base Sequence; D015342:DNA Probes / Q000191:economics / Q000592:standards; D004269:DNA, Bacterial / Q000032:analysis*; D006460:Hemolysin Proteins / Q000235:genetics* / Q000302:isolation & purification; D015345:Oligonucleotide Probes; D010125:Ostreidae / Q000382:microbiology; D016133:Polymerase Chain Reaction; D017747:Seafood / Q000382:microbiology; D012680:Sensitivity and Specificity; D013997:Time Factors; D014736:Vibrio parahaemolyticus / Q000235:genetics* / Q000302:isolation & purification
D001427:Bacterial Toxins; D015342:DNA Probes; D004269:DNA, Bacterial; D006460:Hemolysin Proteins; D015345:Oligonucleotide Probes; C068489:thermostable direct hemolysin; D000469:Alkaline Phosphatase