title: Fast potentiation of glycine receptor channels of intracellular calcium in neurons and transfected cells.
abstract: Inhibitory glycine receptors (GlyRs) are mainly expressed in the spinal cord and in the midbrain, where they control motor and sensory pathways. We describe here a fast potentiation of GlyR by intracellular Ca2+. This phenomenon was observed in rat spinal cord neurons and in transfected human cell lines. Potentiation develops in <100 ms, is proportional to Ca2+ influx, and is characterized by an increase in GlyR apparent affinity for glycine. Phosphorylation and G protein pathways appear not to be involved in the potentiation mechanism. Single-channel recordings in cell-attached and excised patches, as well as whole-cell data suggest the presence of a diffusible cytoplasmic factor that modulates the GlyR channel gating properties. Ca2+-induced potentiation may be important for rapid modulation of glycinergic synapses.
authors.affiliation: Laboratoire de Biologie Cellulaire et Moléculaire du Neurone, Institut Pasteur, Paris, France.
authors.forename: S
authors.initials: S
authors.lastname: Fucile
authors.forename: D
authors.initials: D
authors.lastname: De Saint Jan
authors.forename: L P
authors.initials: LP
authors.lastname: de Carvalho
authors.forename: P
authors.initials: P
authors.lastname: Bregestovski
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004533:Egtazic Acid / Q000031:analogs & derivatives*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000378:metabolism; D005998:Glycine / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D007424:Intracellular Fluid / Q000378:metabolism*; D015640:Ion Channel Gating / Q000187:drug effects; D007668:Kidney; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D010766:Phosphorylation / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D011930:Reaction Time / Q000187:drug effects; D018091:Receptors, AMPA / Q000378:metabolism; D018009:Receptors, Glycine / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D013116:Spinal Cord; D013995:Time; D014162:Transfection
chemical_list: D004791:Enzyme Inhibitors; D018091:Receptors, AMPA; D018009:Receptors, Glycine; D004533:Egtazic Acid; D019204:GTP-Binding Proteins; C025603:1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid; D002118:Calcium; D005998:Glycine