title: Modified salting-out method for DNA isolation from newborn cord blood nucleated cells.
abstract: The present work describes modification of a widely used salting-out procedure to rapidly extract DNA suitable for PCR, using the ARMS method to amplify a target sequence in the beta-globin gene. The salting-out DNA extraction procedure did not completely remove or decrease the presence of inhibitors to PCR in a considerable number of cord blood samples. By introducing a simple phenol/chloroform step, before ethanol precipitation of the nucleic acid, to certain samples, we were able to eliminate or substantially reduce the presence of inhibitors to PCR without having to re-extract the samples.
authors.affiliation: Cátedra y Servicio de Hematología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Hospital Provincial del Centenario, Rosario, Santa Fe, Argentina. nnofuwe@fbioyf.unr.edu.ar
authors.forename: N I
authors.initials: NI
authors.lastname: Noguera
authors.forename: C E
authors.initials: CE
authors.lastname: Tallano
authors.forename: I M
authors.initials: IM
authors.lastname: Bragós
authors.forename: A C
authors.initials: AC
authors.lastname: Milani
mesh_terms: D001773:Blood Cells / Q000737:chemistry*; D011232:Chemical Precipitation; D002725:Chloroform; D004247:DNA / Q000097:blood*; D004587:Electrophoresis, Agar Gel; D000431:Ethanol; D004996:Ethidium; D005312:Fetal Blood / Q000166:cytology*; D005914:Globins / Q000235:genetics*; D006801:Humans; D007202:Indicators and Reagents; D007231:Infant, Newborn; D019800:Phenol; D016133:Polymerase Chain Reaction; D013053:Spectrophotometry
chemical_list: D007202:Indicators and Reagents; D019800:Phenol; D000431:Ethanol; D002725:Chloroform; D005914:Globins; D004247:DNA; D004996:Ethidium