title: Genome-wide analysis of developmental and sex-regulated gene expression profiles in Caenorhabditis elegans.
abstract: We have constructed DNA microarrays containing 17,871 genes, representing about 94% of the 18,967 genes currently annotated in the Caenorhabditis elegans genome. These DNA microarrays can be used as a tool to define a nearly complete molecular profile of gene expression levels associated with different developmental stages, growth conditions, or worm strains. Here, we used these full-genome DNA microarrays to show the relative levels of gene expression for nearly every gene during development, from eggs through adulthood. These expression data can help reveal when a gene may act during development. We also compared gene expression in males to that of hermaphrodites and found a total of 2,171 sex-regulated genes (P < 0.05). The sex-regulated genes provide a global view of the differences between the sexes at a molecular level and identify many genes likely to be involved in sex-specific differentiation and behavior.
authors.affiliation: Department of Developmental Biology, Stanford University Medical Center, Stanford, CA 94305, USA.
authors.forename: M
authors.initials: M
authors.lastname: Jiang
authors.forename: J
authors.initials: J
authors.lastname: Ryu
authors.forename: M
authors.initials: M
authors.lastname: Kiraly
authors.forename: K
authors.initials: K
authors.lastname: Duke
authors.forename: V
authors.initials: V
authors.lastname: Reinke
authors.forename: S K
authors.initials: SK
authors.lastname: Kim
mesh_terms: D000375:Aging / Q000235:genetics; D000818:Animals; D017173:Caenorhabditis elegans / Q000196:embryology / Q000235:genetics* / Q000254:growth & development; D016213:Cyclins / Q000235:genetics; D012734:Disorders of Sex Development / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D017238:Genes, Helminth / Q000235:genetics; D016161:Genes, Retinoblastoma / Q000235:genetics; D016678:Genome; D023281:Genomics; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D011518:Proto-Oncogene Proteins / Q000235:genetics; D012727:Sex Characteristics; D012733:Sex Differentiation / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D051153:Wnt Proteins; D029961:Zebrafish Proteins
chemical_list: D016213:Cyclins; D011518:Proto-Oncogene Proteins; D014157:Transcription Factors; D051153:Wnt Proteins; D029961:Zebrafish Proteins