title: Anoxybacillus pushchinensis gen. nov., sp. nov., a novel anaerobic, alkaliphilic, moderately thermophilic bacterium from manure, and description of Anoxybacillus flavitherms comb. nov.
abstract: A new strictly anaerobic, alkaliphilic, moderately thermophilic, fermentative, spore-forming bacterium, strain K1T, was isolated from manure samples (pH 6-8). Cells were Gram-positive, straight, non-motile rods that grew at temperatures of 37-66 degrees C (optimum at 62 degrees C) and in a pH range of 8.0-10.5 (optimum at 9.5-9.7). The bacterium fermented D-glucose, sucrose, D-fructose, D-trehalose and starch as carbon and energy sources. It required vitamins and its growth is stimulated by yeast extract. The major metabolic products were H2 and acetate. Cells were catalase-negative and could reduce nitrate to nitrite. The G+C content of the DNA was 42.2 mol%. Based on the phenotypic properties and 16S rDNA sequencing and DNA-DNA hybridization data, strain K1T (= DSM 12423T = ATCC 700785T = VKM B-2193T) was assigned to the new genus Anoxybacillus gen. nov., as a representative of a new species, Anoxybacillus pushchinensis sp. nov. 'Bacillus flavothermus' strain d.y., which was found to be closely related to strain K1T, is described as Anoxybacillus flavithermus comb. nov. (type strain = d.y.T = DSM 2641T).
authors.forename: E
authors.initials: E
authors.lastname: Pikuta
authors.forename: A
authors.initials: A
authors.lastname: Lysenko
authors.forename: N
authors.initials: N
authors.lastname: Chuvilskaya
authors.forename: U
authors.initials: U
authors.lastname: Mendrock
authors.forename: H
authors.initials: H
authors.lastname: Hippe
authors.forename: N
authors.initials: N
authors.lastname: Suzina
authors.forename: D
authors.initials: D
authors.lastname: Nikitin
authors.forename: G
authors.initials: G
authors.lastname: Osipov
authors.forename: K
authors.initials: K
authors.lastname: Laurinavichius
mesh_terms: D000693:Anaerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004275:DNA, Ribosomal / Q000032:analysis; D005838:Genotype; D016937:Gram-Positive Endospore-Forming Rods / Q000145:classification* / Q000254:growth & development*; D006863:Hydrogen-Ion Concentration; D008372:Manure / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013696:Temperature
chemical_list: D004275:DNA, Ribosomal; D008372:Manure; D012336:RNA, Ribosomal, 16S