The antibacterial arm of the drosophila innate immune response requires an IkappaB kinase.
The ird5 gene was identified in a genetic screen for Drosophila immune response mutants. Mutations in ird5 prevent induction of six antibacterial peptide genes in response to infection but do not affect the induction of an antifungal peptide gene. Consistent with this finding, Escherichia coli survive 100 times better in ird5 adults than in wild-type animals. The ird5 gene encodes a Drosophila homolog of mammalian IkappaB kinases (IKKs). The ird5 phenotype and sequence suggest that the gene is specifically required for the activation of Relish, a Drosophila NF-kappaB family member.
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Molecular Biology Program, Sloan-Kettering Institute, New York, New York 10021, USA.
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Anderson
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D000818:Animals; D030801:Animals, Genetically Modified; D000900:Anti-Bacterial Agents; D003433:Crosses, Genetic; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics* / Q000276:immunology / Q000502:physiology*; D004926:Escherichia coli / Q000502:physiology; D005260:Female; D005656:Fungal Proteins / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D051550:I-kappa B Kinase; D007814:Larva; D008297:Male; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors / Q000235:genetics*; D001616:beta-Galactosidase / Q000235:genetics
D000900:Anti-Bacterial Agents; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D005656:Fungal Proteins; C052130:GAL4 protein, S cerevisiae; C101626:Rel protein, Drosophila; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D017346:Protein Serine-Threonine Kinases; D051550:I-kappa B Kinase; C496563:IKKbeta protein, Drosophila; D001616:beta-Galactosidase