title: Isolation of Legionella anisa using an amoebic coculture procedure.
abstract: Conventional diagnostic tests for legionellosis were negative for a 61-year-old immunocompromised man with pneumonia. However, coculture of a sputum sample with Acanthamoeba polyphaga amoebae led to the recovery of Legionella anisa. This procedure may be a sensitive and convenient diagnostic method, especially for non-Legionella pneumophila species infections that can be diagnosed only by culture.
authors.affiliation: Unité des Rickettsies, CNRS UPRESA 6020, Faculté de Médecine, 13385 Marseille Cedex 05, France.
authors.forename: B
authors.initials: B
authors.lastname: La Scola
authors.forename: L
authors.initials: L
authors.lastname: Mezi
authors.forename: P J
authors.initials: PJ
authors.lastname: Weiller
authors.forename: D
authors.initials: D
authors.lastname: Raoult
mesh_terms: D000048:Acanthamoeba / Q000254:growth & development / Q000382:microbiology*; D000818:Animals; D006801:Humans; D007875:Legionella / Q000254:growth & development / Q000302:isolation & purification*; D007876:Legionellosis / Q000175:diagnosis / Q000382:microbiology*; D008297:Male; D008875:Middle Aged