title: Isolation and characterization of a Paracentrotus lividus cDNA encoding a stress-inducible chaperonin.
abstract: Chaperonins are ubiquitous proteins that facilitate protein folding in an adenosine triphosphate-dependent manner. Here we report the isolation of a sea urchin cDNA (Plhsp60) coding for mitochondrial chaperonin (Cpn60), whose basal expression is further enhanced by heat shock. The described cDNA corresponds to a full-length mRNA encoding a protein of 582 amino acids, the first 32 of which constitute a putative mitochondrial targeting leader sequence. Comparative analysis has demonstrated that this protein is highly conserved in evolution.
authors.affiliation: Dipartimento di Biologia Cellulare e dello Sviluppo A. Monroy, Palermo, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Gianguzza
authors.forename: M A
authors.initials: MA
authors.lastname: Ragusa
authors.forename: M C
authors.initials: MC
authors.lastname: Roccheri
authors.forename: I
authors.initials: I
authors.lastname: Di Liegro
authors.forename: A M
authors.initials: AM
authors.lastname: Rinaldi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018834:Chaperonin 60 / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000378:metabolism; D018869:Heat-Shock Response; D008928:Mitochondria / Q000235:genetics; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D012617:Sea Urchins / Q000235:genetics* / Q000378:metabolism
chemical_list: D018834:Chaperonin 60; D018076:DNA, Complementary; D012333:RNA, Messenger