Kidney retrieval conditions influence damage to renal medulla: evaluation by proton nuclear magnetic resonance (NMR) pectroscopy.
In the present investigation, the influence of retrieval condition on medullary damage in kidneys was assessed. The isolated perfused pig kidney was used to assess initial renal function from multiorgan donors or single organ donors after cold flush and 24 h cold storage preservation with two preservation solutions: Euro-Collins and University of Wisconsin solutions. Kidneys flushed with cold heparinized saline and immediately perfused were used as a control group. Kidneys were perfused for 90 min at 37.5 degrees C and renal perfusion flow rate, glomerular filtration rate, tubular reabsorption of Na+ and lactate dehydrogenase and N-acetyl-beta-D-glucosaminidase excretion were determined. Ischaemia reperfusion impairment was also determined by 1H NMR (proton nuclear magnetic resonance) spectroscopy. Renal function was significantly decreased in experimental groups when compared to the control group, but there was no significant difference between experimental groups after 24 h cold storage. The release of lactate dehydrogenase in the effluent and the urinary excretion of N-acetyl-beta-D-glucosaminidase were not significantly different after 24 h cold storage. The most relevant resonances determined by 1H NMR spectroscopy were citrate, trimethylamine-N-oxide, lactate, acetate and amino acids. Excretion of these markers was significantly different when compared to biochemical markers. A resonance P (Peak) detected particularly in Euro-Collins solution multiorgan donors after 24 h cold storage was identified and well correlated to renal dysfunction. N-acetyl-beta-D-glucosaminidase spectroscopy, which is a non-invasive and non-destructive technique, is more efficient to assess renal damage than conventional histology and biochemical analysis.
Hauet
T
T
Laboratoire de Transplantation Expérimentale-Institut National de la Recherche Agronomique, Surgères, France. hauet@magneraud.inra.fr
Gibelin
H
H
Godart
C
C
Eugene
M
M
Carretier
M
M
D000818:Animals; D007679:Kidney Medulla / Q000473:pathology*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D011522:Protons; D013552:Swine; D020858:Tissue and Organ Harvesting / Q000379:methods*
D011522:Protons