title: trans-sialidase inhibition assay, a highly sensitive and specific diagnostic test for Chagas' disease.
abstract: For the diagnosis of Chagas' disease, the trans-sialidase inhibition assay was able to resolve the results for samples with borderline results, to detect as positive 60% of samples that were negative by conventional serology, and to discriminate idiopathic from chagasic megaviscera or cardiopathy. No cross-reaction with sera from patients with other diseases was observed.
authors.affiliation: Departamento de Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: A S
authors.initials: AS
authors.lastname: Buchovsky
authors.forename: O
authors.initials: O
authors.lastname: Campetella
authors.forename: G
authors.initials: G
authors.lastname: Russomando
authors.forename: L
authors.initials: L
authors.lastname: Franco
authors.forename: R
authors.initials: R
authors.lastname: Oddone
authors.forename: N
authors.initials: N
authors.lastname: Candia
authors.forename: A
authors.initials: A
authors.lastname: Luquetti
authors.forename: S M
authors.initials: SM
authors.lastname: Gonzalez Cappa
authors.forename: M S
authors.initials: MS
authors.lastname: Leguizamon
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood* / Q000276:immunology; D000953:Antigens, Protozoan / Q000276:immunology*; D001327:Autoimmune Diseases / Q000097:blood / Q000276:immunology; D001938:Brazil / Q000453:epidemiology; D014355:Chagas Disease / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology / Q000276:immunology; D003429:Cross Reactions; D006023:Glycoproteins / Q000037:antagonists & inhibitors* / Q000276:immunology; D006801:Humans; D016773:Leishmaniasis, Cutaneous / Q000097:blood / Q000276:immunology; D008288:Malaria / Q000097:blood / Q000276:immunology; D009439:Neuraminidase / Q000037:antagonists & inhibitors* / Q000276:immunology; D009500:Neutralization Tests / Q000379:methods; D010239:Paraguay / Q000453:epidemiology; D012680:Sensitivity and Specificity; D013587:Syphilis / Q000097:blood / Q000276:immunology; D014349:Trypanosoma cruzi / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D006023:Glycoproteins; C069715:trans-sialidase; D009439:Neuraminidase