title: RNA polymerase--promoter recognition. Specific features of electrostatic potential of "early" T4 phage DNA promoters.
abstract: Comparative analysis of electrostatic potential distribution for "early" T4 phage promoters was undertaken, along with calculation of topography of electrostatic potential around the native and ADP-ribosylated C-terminal domain of RNA polymerase alpha-subunit. The data obtained indicate that there is specific difference in the patterns of electrostatic potential distribution in far upstream regions of T4 promoters differing by their response to ADP-ribosylation of RNA polymerase. A specific change in profiles of electrostatic potential distribution for the native and ADP-ribosylated forms of RNA polymerase alpha-subunit was observed suggesting that this factor may be responsible for modulating T4 promoter activities in response to the enzyme modification.
authors.affiliation: Institute of Cell Biophysics of RAS, Pushchino Moscow Region, Russia. kamzolova@mail.ru
authors.forename: S G
authors.initials: SG
authors.lastname: Kamzolova
authors.forename: V S
authors.initials: VS
authors.lastname: Sivozhelezov
authors.forename: A A
authors.initials: AA
authors.lastname: Sorokin
authors.forename: T R
authors.initials: TR
authors.lastname: Dzhelyadin
authors.forename: N N
authors.initials: NN
authors.lastname: Ivanova
authors.forename: R V
authors.initials: RV
authors.lastname: Polozov
mesh_terms: D000246:Adenosine Diphosphate Ribose / Q000737:chemistry / Q000378:metabolism; D017122:Bacteriophage T4 / Q000235:genetics*; D001483:Base Sequence; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry* / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011487:Protein Conformation; D055672:Static Electricity
chemical_list: D004279:DNA, Viral; D000246:Adenosine Diphosphate Ribose; D012321:DNA-Directed RNA Polymerases; C113275:RNA polymerase alpha subunit