title: Formation, inhibition and clearance of plasmin in vivo.
abstract: A 5 microg/kg bolus of tissue plasminogen activator (t-PA) was infused into 11 healthy subjects followed by measurement of t-PA activity and antigen, PAI-1 activity and antigen, t-PA/PAI-1 complex, plasmin/antiplasmin (PAP) complex and D-dimer over 4 h. Infusion of t-PA resulted in a rise in PAP levels in all subjects from a baseline of 2.4 +/- 1.1 nmol/l to a peak of 5.1 +/- 2.3 nmol/l, but had no effect on plasminogen, antiplasmin or D-dimer levels. Using a kinetic model of plasminogen activation in vivo, the second-order rate constant for t-PA binding to plasminogen was estimated to be 3,100 +/- 1,300 mol(-1)l x s(-1), 200-500 times slower than t-PA accelerated by fibrin. The half-life of PAP was 4.5 +/- 1.6 h. In healthy subjects, the PAP level in plasma represents the average rate of plasminogen activation over the last 2-8 h.
authors.affiliation: Department of Laboratory Medicine, University of Washington, Seattle, Wash., USA. wlc@u.washington.edu
authors.forename: W L
authors.initials: WL
authors.lastname: Chandler
authors.forename: M C
authors.initials: MC
authors.lastname: Alessi
authors.forename: M F
authors.initials: MF
authors.lastname: Aillaud
authors.forename: P
authors.initials: P
authors.lastname: Vague
authors.forename: I
authors.initials: I
authors.lastname: Juhan-Vague
mesh_terms: D000328:Adult; D003198:Computer Simulation; D005260:Female; D005341:Fibrinolysin / Q000378:metabolism*; D005342:Fibrinolysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D008954:Models, Biological
chemical_list: D005341:Fibrinolysin