title: V gamma 2 TCR repertoire overlap in different anatomical compartments of healthy, unrelated rhesus macaques.
abstract: Gammadelta T cells show preferential homing that is characterized by biased TCR repertoire at different anatomical locations. The processes that regulate this compartmentalization are largely unknown. A model that allows repeated multiple sample procurement under different conditions and enables with relatively straightforward extrapolation to a human situation will facilitate our understanding. The peripheral blood Vgamma2 T cell population is the best-characterized human gammadelta T cell subset. To determine its diversity at multiple immunocompartments matching blood, colon, and vagina samples from rhesus macaques were investigated. Four joining segments used in Vgamma2-Jgamma transcripts were identified, including one segment with no human counterpart. Like in humans, the rhesus peripheral blood Vgamma2 TCR repertoire was limited and contained common sequences that were shared by genetically heterogeneous animals. Furthermore, this subset comprised several phylogenetically conserved Vgamma2 complementarity-determining region 3 (CDR3) motifs between rhesus and humans. Common sequences were also found within the colon and vagina of the same animal, and within the peripheral blood and intestine of different unrelated animals. These results validate rhesus macaques as a useful model for gammadelta TCR repertoire and homing studies. Moreover, they provide evidence that the concept of limited but overlapping Vgamma TCR repertoire between unrelated individuals can be extended including the mucosa of the digestive and reproductive tract.
authors.affiliation: Wisconsin Regional Primate Research Center, Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, WI 53715, USA.
authors.forename: A
authors.initials: A
authors.lastname: MacDougall
authors.forename: P
authors.initials: P
authors.lastname: Enders
authors.forename: G
authors.initials: G
authors.lastname: Hatfield
authors.forename: D
authors.initials: D
authors.lastname: Pauza
authors.forename: E
authors.initials: E
authors.lastname: Rakasz
mesh_terms: D020816:Amino Acid Motifs / Q000235:genetics / Q000276:immunology; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012809:Colon, Sigmoid / Q000276:immunology / Q000378:metabolism; D005260:Female; D019675:Genes, T-Cell Receptor gamma; D006801:Humans; D007413:Intestinal Mucosa / Q000276:immunology / Q000378:metabolism; D008253:Macaca mulatta; D008297:Male; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics* / Q000276:immunology*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic / Q000276:immunology; D014621:Vagina / Q000276:immunology / Q000378:metabolism
chemical_list: D016692:Receptors, Antigen, T-Cell, gamma-delta