title: Sensitivity to citrus red mite and the development of asthma.
abstract: OBJECTIVE
  The citrus red mite (Panonychus citri, CRM) is an important allergen in the development of asthma and/or rhinitis in citrus farmers and a common sensitizing allergen among exposed children. The aim of this study was to evaluate CRM-induced skin responses and serum-specific IgE, IgG4, and IgG1 responses, and their role in the development of asthmatic symptoms among exposed adolescents.
  
  
  METHODS
  A total of 100 adolescents between the ages of 15 and 16 years (59 males and 41 females) were randomly recruited. All of the subjects responded to the ISAAC questionnaire themselves. Skin prick tests to CRM were carried out and serum-specific IgE, IgG4, and IgG1 levels were measured using ELISA.
  
  
  RESULTS
  Fourteen subjects (14%) had experienced wheezing during last 12 months. The wheal size formed by CRM was larger than 3 mm in 23 subjects (23%). Serum-specific IgE levels were elevated in 21 subjects (21%), IgG4 levels were elevated in 32 subjects (32%), and IgG1 levels were elevated in five subjects (5%). The prevalence of wheezing during last 12 months was significantly higher in subjects with positive skin responses and serum-specific IgE responses induced by CRM than in those with negative skin responses and low serum IgE responses (30.4% versus 9.0%, P < .05; 33.3% versus 8.7%, P < .05). The prevalence of wheezing, however, did not differ according to serum-specific IgG4 levels (9.4% versus 16.2%, P > .05). Skin responses to CRM showed a significant correlation with serum-specific IgE levels, but not with IgG1 and IgG4 levels. Serum CRM-specific IgE levels correlated with specific IgG1 and IgG4 levels, but no significant correlation was found between serum IgG1 and IgG4 levels.
  
  
  CONCLUSIONS
  The specific IgE responses induced by CRM were prevalent among adolescents living near citrus farms, and may be important in the development of asthma. The specific IgG4 and IgG responses induced by CRM play a minimal role in the development of asthma symptoms.
authors.affiliation: Department of Internal Medicine, Seoul National University College of Medicine, Chongno-gu, Korea.
authors.forename: Y K
authors.initials: YK
authors.lastname: Kim
authors.forename: H W
authors.initials: HW
authors.lastname: Park
authors.forename: H S
authors.initials: HS
authors.lastname: Park
authors.forename: H Y
authors.initials: HY
authors.lastname: Kim
authors.forename: S H
authors.initials: SH
authors.lastname: Kim
authors.forename: J M
authors.initials: JM
authors.lastname: Bai
authors.forename: S H
authors.initials: SH
authors.lastname: Cho
authors.forename: Y Y
authors.initials: YY
authors.lastname: Kim
authors.forename: K U
authors.initials: KU
authors.lastname: Min
mesh_terms: D000293:Adolescent; D000818:Animals; D001249:Asthma / Q000276:immunology*; D002957:Citrus / Q000469:parasitology*; D005260:Female; D006801:Humans; D007114:Immunization; D007073:Immunoglobulin E / Q000097:blood; D008297:Male; D014324:Trombiculidae / Q000276:immunology*
chemical_list: D007073:Immunoglobulin E