[Regeneration of HNP transgenic alfalfa plants by Agrobacterium mediated gene transfer].
Transgenic plants regenerated from cotyledons of M. sativa L. infected using Agrobacterium tumefaciens A281 with plasmid pBF649 containing a gene encoding protein of high sulfur-amino acid content (HNP) were obtained successfully. The plants grew and fertiled well in field. Cotyledon explants were better recipient for transformation of M. sativa L. Environment of suitable temperature (15 degrees C) and high humidity on high viability of the plants transplanted into soil were essential conditions.
Lü
D Y
DY
Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China.
Fan
Y L
YL
Yu
M M
MM
Tang
S X
SX
Hou
N
N
Wang
Q
Q
D000603:Amino Acids, Sulfur / Q000235:genetics*; D018014:Gene Transfer Techniques; D000455:Medicago sativa / Q000235:genetics* / Q000502:physiology; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000502:physiology; D012038:Regeneration; D012231:Rhizobium
D000603:Amino Acids, Sulfur