Use of BACTEC 9240 blood culture system for detection of Brucella melitensis in synovial fluid.
Synovial fluid specimens aspirated from patients with arthritis were inoculated into an aerobic Peds Plus blood culture bottle and monitored by the BACTEC 9240 instrument for 4 weeks. A total of 1,072 synovial fluid cultures were processed, and 15 (0.14%) were positive for Brucella melitensis. A single culture, harboring 1.3 CFU of viable organisms per ml, was missed by the instrument. All 14 positive BACTEC cultures were detected within 3 to 7 days.
Yagupsky
P
P
Clinical Microbiology Laboratories, Soroka Medical Center, Ben-Gurion University of the Negev, Beer-Sheva, Israel. yagupsky@bgumail.bgu.ac.il
Peled
N
N
Press
J
J
D000293:Adolescent; D000328:Adult; D000332:Aerobiosis; D000368:Aged; D001170:Arthritis, Infectious / Q000175:diagnosis / Q000382:microbiology*; D001431:Bacteriological Techniques / Q000295:instrumentation; D001769:Blood; D017347:Brucella melitensis / Q000254:growth & development / Q000302:isolation & purification*; D002006:Brucellosis / Q000175:diagnosis*; D002648:Child; D002675:Child, Preschool; D006801:Humans; D007223:Infant; D007719:Knee Joint; D013582:Synovial Fluid / Q000382:microbiology*