title: Induction of apoptosis by colchicine in taste bud and epithelial cells of the mouse circumvallate papillae.
abstract: Apoptotic cells in the taste buds and epithelia of mouse circumvallate papillae after colchicine treatment were examined by the methods of in situ DNA nick-end labeling, immunocytochemistry, and electron microscopy. After colchicine treatment, numerous positive cells appeared in the taste buds by DNA nick-end labeling, and some epithelial cells in the basal and suprabasal layers in and around the circumvallate papillae also revealed positive staining. Condensed and fragmented nuclei with a high density were occasionally found in the taste bud cells and in the basal and suprabasal layer epithelial cells by electron-microscopic observation. An immunocytochemical reaction for tubulin revealed weak staining in taste bud cells, because of the depolymerization of microtubules, and a decrease of the microtubules in the taste bud cells was observed by electron microscopy. These results indicate that colchicine treatment of mice induces the apoptosis of taste bud and epithelial cells in the circumvallate papillae and dorsal epithelial cells around the circumvallate papillae.
authors.affiliation: Department of Anatomy, School of Dentistry, Health Sciences University of Hokkaido, Tobetsu, Ishikari, Hokkaido 061-0293, Japan. matakeda@hoku-iryo-u.ac.jp
authors.forename: M
authors.initials: M
authors.lastname: Takeda
authors.forename: Y
authors.initials: Y
authors.lastname: Suzuki
authors.forename: N
authors.initials: N
authors.lastname: Obara
authors.forename: Y
authors.initials: Y
authors.lastname: Nagai
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002467:Cell Nucleus / Q000648:ultrastructure; D003078:Colchicine / Q000494:pharmacology*; D004247:DNA / Q000648:ultrastructure; D004847:Epithelial Cells / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D051379:Mice; D008854:Microscopy, Electron; D008870:Microtubules / Q000378:metabolism; D013650:Taste Buds / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D014059:Tongue / Q000166:cytology* / Q000294:innervation* / Q000648:ultrastructure; D014404:Tubulin / Q000276:immunology / Q000378:metabolism
chemical_list: D014404:Tubulin; D004247:DNA; D003078:Colchicine