Extraction and genotyping of Cryptosporidium parvum DNA from fecal smears on glass slides stained conventionally for direct microscope examination.
A method was developed for extracting cryptosporidial DNA from stained fecal smears on glass microscope slides. The correct genotype of Cryptosporidium parvum was amplified by PCR from 89 (85%) of 105 smears following conventional staining but not from negative controls. This technique may have applications for analysis of other infectious agents.
Amar
C
C
Food Safety Microbiology Laboratory, PHLS Division of Gastrointestinal Infections, Central Public Health Laboratory, London, NW9 5HT, SE18 8PF, United Kingdom.
Pedraza-Díaz
S
S
McLauchlin
J
J
D000818:Animals; D003457:Cryptosporidiosis / Q000175:diagnosis* / Q000469:parasitology; D016785:Cryptosporidium parvum / Q000145:classification* / Q000235:genetics; D016054:DNA, Protozoan / Q000032:analysis / Q000235:genetics* / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology*; D005838:Genotype; D006801:Humans; D010274:Parasitology / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D013194:Staining and Labeling / Q000379:methods
D016054:DNA, Protozoan