Structural and phylogenetic analysis of the gamma-actin encoding gene from the penicillin-producing fungus Penicillium chrysogenum.
The nucleotide sequence of a 2994-bp genomic fragment, including the gamma-actin encoding gene from Penicillium chrysogenum, has been determined, showing an open reading frame (ORF) of 1756 bp interrupted by five introns with fungal consensus splice-site junctions. The 5' untranslated region contains a consensus TATA box, five CAAT motifs, and two large pyrimidine stretches. The predicted protein (375 amino acids) revealed high identity to gamma-actins from fungi (>90%), and gene phylogenies support the grouping of P. chrysogenum actin close to those from the majority of the filamentous fungi. The actA gene is present as a single copy in the genome of P. chrysogenum, and its expression is constitutive during penicillin fermentation, showing a single 1.4-kb transcript.
Díez
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B
Area de Biotecnología, Antibióticos S.A. Avenida de Antibióticos 59-61, 24009 León, Spain.
Marcos
A T
AT
Rodríguez
M
M
de la Fuente
J L
JL
Barredo
J L
JL
D020121:5' Untranslated Regions; D000199:Actins / Q000235:genetics*; D003001:Cloning, Molecular; D018628:Gene Dosage; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D010408:Penicillium chrysogenum / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D017422:Sequence Analysis, DNA
D020121:5' Untranslated Regions; D000199:Actins