Chemical nanoprinting: a novel method for fabricating DNA microchips.
We have developed a novel cost-effective procedure, namely 'chemical nanoprinting', for oligonucleotide or cDNA chips manufacture. In this thermo-controlled process, the oligonucleotides, covalently attached to a highly loaded 'master-chip' through disulfide bonds, are chemically transferred to the acrylamide layer mounted on a 'print-chip'. It is demonstrated here that multiple identical print-chips can be produced from a single master-chip. This duplication process is a few hundreds of times faster than any existing methods and the speed of process and cost incurred are independent of the scale of the DNA chips.
Kumar
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Genomics Technology Unit, Center for Genomics Research, Karolinska Institutet, 17177 Stockholm, Sweden.
Liang
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Z
D004247:DNA / Q000032:analysis / Q000138:chemical synthesis*; D008832:Microchemistry / Q000295:instrumentation / Q000379:methods*; D008904:Miniaturization; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*
D004247:DNA