Separation of spiramycin components using high-speed counter-current chromatography.
High-speed counter-current chromatography was successfully applied to the separation of the main components of spiramycin. A 25-mg quantity of sample was separated using a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (3:6:5:5), and the fractions were analyzed by high-performance liquid chromatography and fast atom bombardment mass spectrometry. The separation yielded 13.4, 0.7 and 1.7 mg of spiramycins I, II, and III with purities of 98.2, 92.3 and 97.4%, respectively.
Oka
H
H
Aichi Prefectural Institute of Public Health, Nagoya, Japan. hisaooka@alles.or.jp
Harada
K
K
Suzuki
M
M
Ito
Y
Y
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000379:methods*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D015572:Spiramycin / Q000737:chemistry / Q000302:isolation & purification*
D000900:Anti-Bacterial Agents; D015572:Spiramycin