Immune response to 17beta-estradiol involved in polymer gels: antigen specificity and affinity of hybridoma clones.
The immunogenic properties of 17beta-estradiol, immobilized in negatively charged polymer gels, were investigated, and the specificity of antibodies produced was analyzed. The polymer gels developed were composed of a hydrophobic estradiol core surrounded by hydrophilic polyanions as corona. As an immunogen, it was conceived to function via a dual mode, that is as a hapten-delivery system (prolongation effect) and as a polyelectrolyte adjuvant. Polymer gels containing estradiol appeared to possess a high estradiol-specific immunogenicity even without the addition of traditional adjuvants. A comparative study of estradiol trapped in polymer gels versus estradiol conjugated to bovine serum albumin (BSA.E) + Incomplete Freund's Adjuvant (IFA) mixtures revealed similar immunogenic properties in terms of induction of specific antibodies. Following a short immunization procedure based on the use of 17beta-estradiol immobilized in polymer gels, we developed 10 specific monoclonal antibodies with Kd values ranging between 1.2 X 10(-7) and 8 X 10(-8) M.
Başalp
A
A
Research Institute for Genetic Engineering and Biotechnology, Tübitak-Marmara Research Center, Kocaeli, Turkey.
Mustafaeva
Z
Z
Mustafaev
M
M
Bermek
E
E
D000276:Adjuvants, Immunologic / Q000494:pharmacology / Q000592:standards; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification; D000915:Antibody Affinity; D000918:Antibody Specificity; D004958:Estradiol / Q000276:immunology*; D005620:Freund's Adjuvant / Q000737:chemistry / Q000276:immunology; D005782:Gels; D006825:Hybridomas; D008055:Lipids; D008722:Methods; D051379:Mice; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000276:immunology; D013256:Steroids / Q000276:immunology
D000276:Adjuvants, Immunologic; D000911:Antibodies, Monoclonal; D005782:Gels; D008055:Lipids; D013256:Steroids; C114843:incomplete Freund's adjuvant; D012710:Serum Albumin, Bovine; D004958:Estradiol; D005620:Freund's Adjuvant