title: Integrative module of the high-pathogenicity island of Yersinia.
abstract: The high-pathogenicity island of Yersinia pestis (Yps HPI) encodes virulence-associated genes involved in siderophore yersiniabactin-mediated iron uptake. The Yps HPI contains a P4-type integrase (Int-HPI), associated with the asn-tRNA locus, and is flanked by 17 bp direct repeats. We constructed a minimal integrative module of the pathogenicity island carrying the reconstituted 266 bp attP (POP') attachment site derived from putative attR and attL junctions of the Yps HPI and the functional int-HPI gene from Y. pestis KUMA. The attP-int-HPI module recombined efficiently, site specifically and RecA independently with the bacterial attB site present either in the chromosome (asn-tDNA) or on a plasmid, with no preference for a certain asn-tRNA gene. The excision of the integrated suicide plasmid carrying the integrative module, on the other hand, was a rare event and could be demonstrated only by polymerase chain reaction. Analysis of the 5' terminus of the transcript for int-HPI revealed that the integration of attP-int-HPI was coupled with the replacement of the endogenous int-HPI promoter, localized in the P' part of the attP site, by the adjacent asn-tRNA promoter. These results suggest that two alternative promoters control integration and excision of the HPI by its integrase.
authors.affiliation: Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, Petterkofer Str. 9a, 80336 München, Germany. rakin@m3401.mpk.med.uni-muenchen.de
authors.forename: A
authors.initials: A
authors.lastname: Rakin
authors.forename: C
authors.initials: C
authors.lastname: Noelting
authors.forename: P
authors.initials: P
authors.lastname: Schropp
authors.forename: J
authors.initials: J
authors.lastname: Heesemann
mesh_terms: D001287:Attachment Sites, Microbiological; D001483:Base Sequence; D019426:Integrases / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic; D014774:Virulence / Q000235:genetics; D015010:Yersinia pestis / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity*
chemical_list: D019426:Integrases