title: Accuracy of the VITEK 2 system to detect glycopeptide resistance in enterococci.
abstract: We evaluated the accuracy of the VITEK 2 fully automated system to detect and identify glycopeptide-resistant enterococci (GRE) compared to a reference agar dilution method. The sensitivity of vancomycin susceptibility testing with VITEK 2 for the detection of vanA, vanB, and vanC1 strains was 100%. The sensitivity of vancomycin susceptibility testing of vanC2 strains was 77%. The sensitivity of teicoplanin susceptibility testing of vanA strains was 90%. Of 80 vanC enterococci, 78 (98%) were correctly identified by VITEK 2 as Enterococcus gallinarum/Enterococcus casseliflavus. Since the identification and susceptibility data are produced within 3 and 8 h, respectively, VITEK 2 appears a fast and reliable method for detection of GRE in microbiology laboratories.
authors.affiliation: Erasmus University Medical Center Rotterdam, Rotterdam, The Netherlands. vandenbraak@bacl.azr.nl
authors.forename: N
authors.initials: N
authors.lastname: van Den Braak
authors.forename: W
authors.initials: W
authors.lastname: Goessens
authors.forename: A
authors.initials: A
authors.lastname: van Belkum
authors.forename: H A
authors.initials: HA
authors.lastname: Verbrugh
authors.forename: H P
authors.initials: HP
authors.lastname: Endtz
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D016983:Enterococcus / Q000187:drug effects* / Q000235:genetics; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D012680:Sensitivity and Specificity; D017334:Teicoplanin / Q000494:pharmacology*; D014166:Transferases; D014640:Vancomycin / Q000494:pharmacology*; D020713:Vancomycin Resistance / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D017334:Teicoplanin; D014640:Vancomycin; D014166:Transferases; C019917:3-deoxy-D-manno-octulosonate transferase