title: Children with untreated food allergy express a relative increment in the density of duodenal gammadelta+ T cells.
abstract: BACKGROUND
  We investigated whether children with food allergy (FA) express increased densities of intraepithelial gammadelta+ T cells similarly to subjects with celiac disease.
  
  
  METHODS
  The duodenal specimens taken by gastroduodenoscopy from 20 children with untreated FA, 17 with treated FA, 12 with celiac disease (CD) and 12 controls were studied with monoclonal antibodies and a three-layer peroxidase staining method.
  
  
  RESULTS
  The subjects with untreated FA expressed equal densities of total intraepithelial CD3+ and alpha/beta+ T cells, but significantly higher densities of gammadelta+ cells than the subjects currently on an elimination diet for FA or the controls. Accordingly, their gammadelta+/CD3+ ratio was higher. On the other hand, the results differed clearly from CD, where all the three cell populations showed high densities. Another finding that discriminated the subjects with FA from the CD patients was endoscopic examination. Lymphonodular hyperplasia (LNH) of the duodenal bulb with a normal villous pattern was demonstrated in 14 (70%) of the 20 subjects with untreated FA and in 8 (47%) of the 17 with treated FA, but in none of the celiac patients or controls. Surprisingly, the biopsy samples from the subjects with FA showed quite normal histological findings.
  
  
  CONCLUSIONS
  According to this preliminary observation, high densities of intraepithelial gammadelta+ T cells and a significantly elevated gammadelta+/CD3+ ratio are associated with untreated FA. If seen LNH in a gastroduodenoscopy and/or increased densities of gammadelta+ T cells in the biopsy specimen, the possibility of gastrointestinal FA should be reliably assessed by a food challenge.
authors.affiliation: Dept. of Pediatrics, University of Oulu, Finland.
authors.forename: J
authors.initials: J
authors.lastname: Kokkonen
authors.forename: K
authors.initials: K
authors.lastname: Holm
authors.forename: T J
authors.initials: TJ
authors.lastname: Karttunen
authors.forename: M
authors.initials: M
authors.lastname: Mäki
mesh_terms: D000293:Adolescent; D017252:CD3 Complex / Q000032:analysis; D002446:Celiac Disease / Q000276:immunology* / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D004386:Duodenum / Q000276:immunology* / Q000473:pathology; D016099:Endoscopy, Gastrointestinal; D005260:Female; D005512:Food Hypersensitivity / Q000276:immunology* / Q000473:pathology / Q000628:therapy; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D007413:Intestinal Mucosa / Q000276:immunology* / Q000473:pathology; D018655:Lymphocyte Count; D008297:Male; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis*; D016176:T-Lymphocyte Subsets
chemical_list: D017252:CD3 Complex; D006684:HLA-DR Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta