U-74389G suppresses lipid peroxidation and apoptosis following focal cerebral ischemia and reperfusion in rats.
OBJECTIVE
To study the antioxidant and antiapoptotic effects of U-74389G on ischemic brain in rats.


METHODS
Right focal cerebral ischemia and reperfusion was induced by suturing in 103 male SD rats weighing 280-310 g. To determine LPO in the cortex and striatum, we randomly divided 78 rats into 5 groups: group 1, sham-operative group (6 rats); group 2, control (18); group 3, U-74389G administration before ischemia (18); group 4, U-74389G administration before reperfusion; group 5, CS-4 vehicle as negative control. Groups 2-5 were further divided into 3 subgroups according to the duration of reperfusion. Another 25 rats were similarly grouped (5) to detect apoptosis with TUNEL stain.


RESULTS
The concentrations of MDA in groups 3 and 4 were reduced and the activities of SOD and concentrations of GSH were partially restored as compared with the control group. These effects were more significant in group 3 than in group 4. Otherwise, CS-4 can not provide protective effects. The number of apoptotic cells in groups 3 (P < 0.01) and 4 (P < 0.05) was significantly reduced as compared with the control group.


CONCLUSIONS
U-74389G has antioxidant and antiapoptotic effects on ischemic brain in rats. The administration of U-74389G before the onset of ischemia was more useful than that before the onset of reperfusion.
Zhang
F
F
Department of Neurosurgery, Huashan Hospital, Shanghai Medical University, Shanghai 200040, China.
Zhou
L
L
Huang
F
F
Liang
Y
Y
Zhang
G
G
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002546:Ischemic Attack, Transient / Q000378:metabolism* / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D011281:Pregnatrienes / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology
D000975:Antioxidants; D011281:Pregnatrienes; C074965:U 74389F