Deep venous thrombosis: leukocyte rheology at baseline and after in vitro activation.
We evaluated leukocyte rheology, expressed as leukocyte filtration, polymorphonuclear (PMN) membrane fluidity and cytosolic Ca2+ concentration in subjects with acute deep venous leg thrombosis (DVT). In 14 subjects with leg DVT we examined the leukocyte filtration [unfractionated, mononuclear cells (MN), PMNs], PMN membrane fluidity and PMN cytosolic Ca2+ concentration. Subsequently, we evaluated the same PMN variables after in vitro chemotactic activation with 4-phorbol-12-myristate-13-acetate. At baseline, we observed a significant difference in the filtration of unfractionated and MNs and in PMN cytosolic Ca2+ concentration. After PMN activation, a significant variation, greater in DVT subjects, was present in PMN filtration at 5 and 15 min. In normals, no variation was present in PMN membrane fluidity or cytosolic Ca2+ concentration after activation, while in subjects with DVT we found a significant variation in both PMN parameters. These results underline that there is a systemic leukocyte functional alteration in DVT.
LoPresti
R
R
Istituto di Clinica Medica a Malattie Cardiovascolari, Università di Palermo, Palermo, Italia.
Ferrara
F
F
Canino
B
B
Montana
M
M
Caimi
G
G
D000328:Adult; D000368:Aged; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D002634:Chemotaxis, Leukocyte / Q000502:physiology; D005260:Female; D006801:Humans; D007962:Leukocytes / Q000473:pathology / Q000502:physiology*; D008297:Male; D008560:Membrane Fluidity; D008875:Middle Aged; D012212:Rheology; D013924:Thrombophlebitis / Q000097:blood* / Q000473:pathology / Q000503:physiopathology
D002118:Calcium