title: The autosomal dominant hypophosphatemic rickets (ADHR) gene is a secreted polypeptide overexpressed by tumors that cause phosphate wasting.
abstract: The gene mutated in autosomal dominant hypophosphatemic rickets (ADHR), a phosphate wasting disorder, has been identified as FGF-23, a protein that shares sequence homology with fibroblast growth factors (FGFs). Patients with ADHR display many of the clinical and laboratory characteristics that are observed in patients with oncogenic hypophosphatemic osteomalacia (OHO), a disorder thought to arise by the secretion of a phosphate wasting factor from different mesenchymal tumors. In the present studies, we therefore investigated whether FGF-23 is a secreted factor and whether it is abundantly expressed in OHO tumors. After transient transfection of OK-E, COS-7, and HEK293 cells with the plasmid encoding full-length FGF-23, all three cell lines efficiently secreted two protein species into the medium that were approximately 32 and 12 kDa upon SDS-PAGE and subsequent Western blot analysis using an affinity-purified polyclonal antibody to FGF-23. Furthermore, Northern blot analysis using total RNA from five different OHO tumors revealed extremely high levels of FGF-23 mRNA, and Western blot analysis of extracts from a sixth tumor detected the 32 kDa FGF-23 protein species. In summary, FGF-23, the gene mutated in ADHR, is a secreted protein and its mRNA is abundantly expressed by several different OHO tumors. Our findings indicate that FGF-23 may be a candidate phosphate wasting factor, previously designated "phosphatonin".
authors.affiliation: Department of Medicine, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA.
authors.forename: K E
authors.initials: KE
authors.lastname: White
authors.forename: K B
authors.initials: KB
authors.lastname: Jonsson
authors.forename: G
authors.initials: G
authors.lastname: Carn
authors.forename: G
authors.initials: G
authors.lastname: Hampson
authors.forename: T D
authors.initials: TD
authors.lastname: Spector
authors.forename: M
authors.initials: M
authors.lastname: Mannstadt
authors.forename: B
authors.initials: B
authors.lastname: Lorenz-Depiereux
authors.forename: A
authors.initials: A
authors.lastname: Miyauchi
authors.forename: I M
authors.initials: IM
authors.lastname: Yang
authors.forename: O
authors.initials: O
authors.lastname: Ljunggren
authors.forename: T
authors.initials: T
authors.lastname: Meitinger
authors.forename: T M
authors.initials: TM
authors.lastname: Strom
authors.forename: H
authors.initials: H
authors.lastname: Jüppner
authors.forename: M J
authors.initials: MJ
authors.lastname: Econs
mesh_terms: D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D006224:Cricetinae; D000089703:Fibroblast Growth Factor-23; D005346:Fibroblast Growth Factors / Q000235:genetics*; D006801:Humans; D007015:Hypophosphatemia, Familial / Q000150:complications / Q000235:genetics* / Q000503:physiopathology; D008637:Mesenchymoma / Q000150:complications / Q000503:physiopathology*; D008969:Molecular Sequence Data; D010018:Osteomalacia / Q000503:physiopathology; D012333:RNA, Messenger / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: C000717427:FGF23 protein, human; D012333:RNA, Messenger; D011994:Recombinant Proteins; D005346:Fibroblast Growth Factors; D000089703:Fibroblast Growth Factor-23