Modulation of WHSC2 expression in human endothelial cells.
WHSC2, a novel gene recently isolated within the critical region of Wolf-Hirschhorn syndrome, is expressed in endothelial cells. WHSC2 is downregulated by HIV-1 Tat, whereas it is not modulated by angiogenic and pro-differentiative molecules. WHSC2 encodes a basic polypeptide of 528 amino acids. The in vitro translated protein shows a molecular weight of 57 kDa. WHSC2 has two nuclear translocation sequences which actively mediate its transport to the nucleus, as shown in whsc2-GFP-transfected NIH-3T3. We also found a helix-loop-helix (HLH) motif in region 130-185. Since members of the HLH family control differentiation and cell cycle progression, we hypothesize that WHSC2 may function as a transcriptional repressor.
Mariotti
M
M
Department of Preclinical Sciences-LITA Vialba, University of Milano, Via G.B. Grassi, 74, 20157, Milan, Italy.
Manganini
M
M
Maier
J A
JA
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D002453:Cell Cycle; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D016228:Endothelial Growth Factors / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D015696:Gene Products, tat / Q000494:pharmacology; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D006133:Growth Substances / Q000494:pharmacology*; D015497:HIV-1; D018257:Helix-Loop-Helix Motifs; D017228:Hepatocyte Growth Factor / Q000494:pharmacology; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics; D008222:Lymphokines / Q000494:pharmacology; D051379:Mice; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D017433:Protein Structure, Secondary; D021381:Protein Transport; D011506:Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D035602:Transcriptional Elongation Factors; D014162:Transfection; D014471:Umbilical Veins; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D054322:tat Gene Products, Human Immunodeficiency Virus
D016228:Endothelial Growth Factors; D015696:Gene Products, tat; D006133:Growth Substances; D008164:Luminescent Proteins; D008222:Lymphokines; C120899:NELFA protein, human; D011506:Proteins; D011994:Recombinant Proteins; D035602:Transcriptional Elongation Factors; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D054322:tat Gene Products, Human Immunodeficiency Virus; D016222:Fibroblast Growth Factor 2; D049452:Green Fluorescent Proteins; D017228:Hepatocyte Growth Factor