Immunodiagnosis of human fascioliasis by an enzyme-linked immunosorbent assay (ELISA) and a micro-ELISA.
Enzyme-linked immunosorbent assay (ELISA) and micro-ELISA were evaluated for their ability to detect anti-Fasciola hepatica antibodies in humans by using excretory-secretory antigen. The sensitivity of each method was 100%, but the specificity was 100% for ELISA and 97% for micro-ELISA. The micro-ELISA could be used as a screening assay and ELISA could be used as a confirmatory method for the serodiagnosis of human fascioliasis.
Carnevale
S
S
Departamento de Parasitología Sanitaria, Instituto Nacional de Parasitología, ANLIS "Dr. Carlos G. Malbrán," 1281 Buenos Aires, Argentina.
Rodríguez
M I
MI
Santillán
G
G
Labbé
J H
JH
Cabrera
M G
MG
Bellegarde
E J
EJ
Velásquez
J N
JN
Trgovcic
J E
JE
Guarnera
E A
EA
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood* / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005211:Fascioliasis / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D006801:Humans; D007159:Immunologic Tests / Q000379:methods
D000909:Antibodies, Helminth; D000947:Antigens, Helminth