title: Circulating nucleic acids of Chlamydia pneumoniae and cytomegalovirus in patients undergoing coronary angiography.
abstract: Peripheral blood mononuclear cells from 208 consecutive patients undergoing elective coronary angiography or angioplasty were collected before, immediately after, and 4 h after the procedure. Nucleic acids of Chlamydia pneumoniae and of cytomegalovirus (CMV) were detected by PCR and confirmed by hybridization. Circulating C. pneumoniae DNA was identified in 24 patients (11.5%) and was associated with current smoking (odds ratio [OR] = 4.5, 95% confidence interval [CI] = 1.6 to 12.2, P = 0.004) but not with arterial narrowing on coronary angiogram or with serological results positive for C. pneumoniae. Circulating CMV DNA was identified in 36 patients (17.3%) and was associated with anti-CMV immunoglobulin G (OR = 2.7, 95% CI = 1.2 to 6.3, P = 0.02) but not with angiographic arterial narrowing or with the need for revascularization. Neither C. pneumoniae nor CMV DNA detection increased after angioplasty, a procedure in which endothelium is disrupted. Larger prospective studies are needed to determine the prognostic significance of DNA detection.
authors.affiliation: Hamilton Regional Laboratory Medicine Programme, McMaster University, Hamilton, Ontario, Canada. smiejam@mcmaster.ca
authors.forename: M
authors.initials: M
authors.lastname: Smieja
authors.forename: S
authors.initials: S
authors.lastname: Chong
authors.forename: M
authors.initials: M
authors.lastname: Natarajan
authors.forename: A
authors.initials: A
authors.lastname: Petrich
authors.forename: L
authors.initials: L
authors.lastname: Rainen
authors.forename: J B
authors.initials: JB
authors.lastname: Mahony
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015906:Angioplasty, Balloon, Coronary; D000907:Antibodies, Bacterial / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D016993:Chlamydophila pneumoniae / Q000302:isolation & purification*; D017023:Coronary Angiography; D003327:Coronary Disease / Q000000981:diagnostic imaging / Q000382:microbiology* / Q000628:therapy; D003587:Cytomegalovirus / Q000276:immunology / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000097:blood*; D004279:DNA, Viral / Q000097:blood*; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D016133:Polymerase Chain Reaction / Q000379:methods; D011379:Prognosis; D012044:Regression Analysis; D012907:Smoking
chemical_list: D000907:Antibodies, Bacterial; D000914:Antibodies, Viral; D004269:DNA, Bacterial; D004279:DNA, Viral; D007070:Immunoglobulin A; D007074:Immunoglobulin G