title: Critical hematocrit in intestinal tissue oxygenation during severe normovolemic hemodilution.
abstract: BACKGROUND
  A critical point in oxygen supply for microvascular oxygenation during normovolemic hemodilution has not been identified. The relation between organ microvascular oxygen partial pressure (microPO2) and organ oxygen consumption (VO2) during a decreasing oxygen delivery (DO2) is not well understood. The present study was designed to determine the systemic hematocrit and organ DO2 values below which organ microPO2 and VO2 cannot be preserved by regulatory mechanisms during normovolemic hemodilution.
  
  
  METHODS
  Eighteen male Wistar rats were randomized between an experimental group (n = 12), in which normovolemic hemodilution was performed with pasteurized protein solution (PPS), and a control group (n = 6). Systemic hemodynamic and intestinal oxygenation parameters were monitored. Intestinal microPO2 was measured using the oxygen-dependent quenching of palladium-porphyrin phosphorescence.
  
  
  RESULTS
  Baseline values in hemodilution and control group were similar. Hemodilution decreased hematocrit to 6.2 +/- 0.8% (mean +/- SD). Constant central venous pressure measurements suggested maintenance of isovolemia. Despite an increasing mesenteric blood flow, intestinal DO2 decreased immediately. Initially, microPO2 was preserved, whereas mesenteric venous PO2 (P(mv)O2) decreased; below a hematocrit of 15%, microPO2 decreased significantly below P(mv)O2. Critical DO2 was 1.5 +/- 0.5 ml x kg(-1) x min(-1) for VO2, and 1.6 +/- 0.5 ml x kg(-1) x min(-1) for microPO2. Critical hematocrit values for VO2 and microPO2 were 15.8 +/- 4.6% and 16.0 +/- 3.5%, respectively.
  
  
  CONCLUSIONS
  Intestinal microPO2 and VO2 were limited by a critical decrease in DO2 and hematocrit at the same time. Beyond these critical points not only shunting of oxygen from the microcirculation could be demonstrated, but also a significant correlation between intestinal microPO2 and VO2.
authors.affiliation: Department of Anesthesiology, Academic Medical Center, University of Amsterdam, The Netherlands. j.vanbommel@amc.uva.nl
authors.lastname: van Bommel J
authors.forename: M
authors.initials: M
authors.lastname: Siegemund
authors.forename: C P
authors.initials: CP
authors.lastname: Henny
authors.forename: A
authors.initials: A
authors.lastname: Trouwborst
authors.forename: C
authors.initials: C
authors.lastname: Ince
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D006400:Hematocrit; D006438:Hemodilution; D006439:Hemodynamics; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D010101:Oxygen Consumption; D051381:Rats; D017208:Rats, Wistar