title: Regulation by chemokines of circulating dendritic cell precursors, and the formation of portal tract-associated lymphoid tissue, in a granulomatous liver disease.
abstract: We have studied the recruitment and roles of distinct dendritic cell (DC) precursors from the circulation into Propionibacterium acnes-induced granulomas in mouse liver. During infection, F4/80(-)B220(-)CD11c(+) DC precursors appeared in the circulation, migrated into the perisinusoidal space, and matured within newly formed granulomas. Recruited DCs later migrated to the portal area to interact with T cells in what we term "portal tract-associated lymphoid tissue" (PALT). Macrophage inflammatory protein 1alpha attracted blood DC precursors to the sinusoidal granuloma, whereas secondary lymphoid organ chemokine (SLC) attracted mature DCs to the newly identified PALT. Anti-SLC antibody diminished PALT expansion while exacerbating granuloma formation. Therefore, circulating DC precursors can migrate into a solid organ like liver, and participate in the granulomatous reaction in response to specific chemokines.
authors.affiliation: Department of Molecular Preventive Medicine, School of Medicine The University of Tokyo, Bunkyoku, Tokyo 113-0033, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yoneyama
authors.forename: K
authors.initials: K
authors.lastname: Matsuno
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: M
authors.initials: M
authors.lastname: Murai
authors.forename: M
authors.initials: M
authors.lastname: Itakura
authors.forename: S
authors.initials: S
authors.lastname: Ishikawa
authors.forename: G
authors.initials: G
authors.lastname: Hasegawa
authors.forename: M
authors.initials: M
authors.lastname: Naito
authors.forename: H
authors.initials: H
authors.lastname: Asakura
authors.forename: K
authors.initials: K
authors.lastname: Matsushima
mesh_terms: D000818:Animals; D001483:Base Sequence; D018845:CD11 Antigens / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects / Q000276:immunology; D054421:Chemokine CCL21; D054407:Chemokine CCL4; D018925:Chemokines / Q000235:genetics / Q000494:pharmacology* / Q000502:physiology; D019742:Chemokines, CC / Q000235:genetics / Q000502:physiology; D017931:DNA Primers / Q000235:genetics; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology* / Q000473:pathology*; D005260:Female; D006099:Granuloma / Q000276:immunology* / Q000473:pathology*; D008107:Liver Diseases / Q000276:immunology* / Q000473:pathology*; D008221:Lymphoid Tissue / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D019402:Macrophage Inflammatory Proteins / Q000235:genetics / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D011425:Propionibacterium acnes / Q000472:pathogenicity; D013234:Stem Cells / Q000187:drug effects / Q000276:immunology / Q000473:pathology
chemical_list: D018845:CD11 Antigens; C515479:Ccl21c protein, mouse; D054421:Chemokine CCL21; D054407:Chemokine CCL4; D018925:Chemokines; D019742:Chemokines, CC; D017931:DNA Primers; D019402:Macrophage Inflammatory Proteins