Treatment of surgically induced acute liver failure with transplantation of highly differentiated immortalized human hepatocytes.
Primary human hepatocytes are an ideal source of hepatic function in bioartficial liver (BAL), but the shortage of human livers available for hepatocyte isolation limits this modality. To resolve this issue, primary human fetal hepatocytes were immortalized using simian virus 40 large T antigen. One of the immortal cell lines, OUMS-29, showed highly differentiated liver functions. Intrasplenic transplantation of OUMS-29 cells protected 90% hepatectomized rats from hyperammonemia and significantly prolonged their survival. Essentially unlimited availability of OUMS-29 cells supports their clinical use for BAL treatment.
Kobayashi
N
N
Department of Cell Biology, Institute of Molecular and Cellular Biology, Okayama University Medical School, Japan. ntanaka@med.okayama-u.ac.jp
Miyazaki
M
M
Fukaya
K
K
Inoue
Y
Y
Sakaguchi
M
M
Noguchi
H
H
Matsumura
T
T
Watanabe
T
T
Totsugawa
T
T
Tanaka
N
N
Namba
M
M
D000641:Ammonia / Q000097:blood; D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D002454:Cell Differentiation; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D006498:Hepatectomy; D022781:Hepatocytes / Q000166:cytology / Q000637:transplantation*; D006801:Humans; D008099:Liver / Q000196:embryology; D017114:Liver Failure, Acute / Q000097:blood / Q000209:etiology / Q000601:surgery*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000601:surgery; D015996:Survival Rate
D000952:Antigens, Polyomavirus Transforming; D000641:Ammonia