title: Interaction between lectins and neoglycoproteins containing new sialylated glycosynthons.
abstract: Neoglycoconjugates are useful tools to study carbohydrate/protein interactions. In order to discover new lectins, to define their fine specificity or to study their intracellular trafficking, there is a need for neoglycoconjugates containing complex oligosaccharides. We recently set up a simple way to transform native oligosaccharides into glycosynthons. The present paper describes i) the synthesis of such glycosynthons starting with sialylated oligosides, ii) the preparation of sialylated neoglycoproteins and iii) their binding to sialic acid-specific lectins assessed by surface plasmon resonance experiments.
authors.affiliation: Glycobiologie, Vectorologie et Trafic Intracellulaire, Centre de Biophysique Moléculaire, CNRS et Université d'Orléans, France.
authors.forename: E
authors.initials: E
authors.lastname: Duverger
authors.forename: A
authors.initials: A
authors.lastname: Coppin
authors.forename: G
authors.initials: G
authors.lastname: Strecker
authors.forename: M
authors.initials: M
authors.lastname: Monsigny
mesh_terms: D001665:Binding Sites; D002240:Carbohydrate Sequence; D006023:Glycoproteins / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D066298:In Vitro Techniques; D037102:Lectins / Q000737:chemistry* / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D012794:Sialic Acids / Q000737:chemistry; D020349:Surface Plasmon Resonance
chemical_list: D006023:Glycoproteins; D037102:Lectins; D012794:Sialic Acids