Inhibition of OM pathogens by alpha-hemolytic streptococci from healthy children, children with SOM and children with rAOM.
The present study was undertaken to elucidate the inhibitory activity of the normal nasopharyngeal flora against the three most common otitis media (OM) pathogens in healthy children, children with secretory otitis media (SOM) and children with recurrent otitis media (rAOM). Isolates of alpha-hemolytic streptococci (AHS) and OM pathogens were recovered from the tubal orifice in each child. The samples were taken from 20 healthy children under general anesthesia, from 19 children with SOM and 20 children with rAOM. The method used to test the bacterial interference in vitro was a modified agar overlay method. The AHS sampled from the tubal orifice of the healthy children were able to inhibit 92% of the S. peumoniae isolates, 74% of the non-typable H. influenzae isolates and 89% of the M. catarrhalis isolates. The corresponding figures for children with SOM and children with rAOM were: 73% of the S. pneumoniae isolates, 58 and 54% of the non-typable H. influenzae isolates and 86 and 89% of the M. catarrhalis isolates. The AHS from children with SOM and children with rAOM were significantly less capable of inhibiting the S. peumoniae and the H. influenzae isolates (P<0.001). There was no significant difference between the three groups of children regarding inhibitory activity against M. catarrhalis. The results suggest that the inhibitory activity of the normal bacterial flora at the tubal orifice against pneumococci and H. influenzae may be reduced in children with SOM and rAOM.
Tano
K
K
Department of Clinical Sciences, Otorhinolaryngology, Umeâ University and ENT Department, Sunderby Hospital, S-971 80, Luleå, Sweden. krister.tano@nll.se
Grahn-Håkansson
E
E
Holm
S E
SE
Hellström
S
S
D000208:Acute Disease; D000898:Antibiosis; D002648:Child; D002675:Child, Preschool; D005260:Female; D006193:Haemophilus influenzae / Q000254:growth & development; D006801:Humans; D007223:Infant; D008297:Male; D001936:Moraxella catarrhalis / Q000254:growth & development; D009305:Nasopharynx / Q000382:microbiology*; D010033:Otitis Media / Q000382:microbiology*; D010034:Otitis Media with Effusion / Q000382:microbiology; D012008:Recurrence; D013291:Streptococcus / Q000302:isolation & purification / Q000502:physiology*; D013296:Streptococcus pneumoniae / Q000254:growth & development