Nucleotide sequence and characterization of a novel cefotaxime-hydrolyzing beta-lactamase (CTX-M-10) isolated in Spain.
A cefotaxime-resistant, ceftazidime-susceptible Escherichia coli isolate was obtained from a patient with sepsis in 1997, from which a beta-lactamase with a pI of 8.1 was cloned. Cephaloridine and cefotaxime relative hydrolysis rates were 167 and 81, respectively (penicillin G rate = 100), whereas ceftazidime hydrolysis was not detected. The nucleotide sequence revealed a bla gene related to that coding for CTX-M-3. Despite 21 nucleotide substitutions, only 2 determined amino acid changes (Ala27Val and Arg38Gln). The amino acid sequence identity between this enzyme, designated CTX-M-10, and the chromosomal beta-lactamase of Kluyvera ascorbata was 81%.
Oliver
A
A
Servicio de Microbiología, Hospital Ramón y Cajal, Madrid, Spain.
Pérez-Díaz
J C
JC
Coque
T M
TM
Baquero
F
F
Cantón
R
R
D002439:Cefotaxime / Q000378:metabolism / Q000494:pharmacology; D002509:Cephaloridine / Q000378:metabolism / Q000494:pharmacology; D002511:Cephalosporins / Q000378:metabolism / Q000494:pharmacology; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D004927:Escherichia coli Infections / Q000382:microbiology; D005798:Genes, Bacterial / Q000235:genetics; D006801:Humans; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D001618:beta-Lactamases / Q000737:chemistry* / Q000235:genetics*
D002511:Cephalosporins; D004269:DNA, Bacterial; C425114:beta-lactamase CTX-M-10; D001618:beta-Lactamases; D002509:Cephaloridine; D002439:Cefotaxime