title: Hydrogen peroxide triggers the expression of Fas/FasL in astrocytoma cell lines and augments apoptosis.
abstract: Fas expression increases in perinecrotic areas of glioblastoma. In this study the up-regulation of Fas/FasL by oxidative stress was shown. H(2)O(2) exposure increased Fas expression in two astrocytoma cell lines and cells became sensitive to agonistic anti-Fas antibody. FasL was up-regulated in astrocytoma cells. Apoptosis of Molt-4 cells was augmented by astrocytoma cells pretreated with H(2)O(2). Our findings suggest up-regulation of Fas in astrocytoma cells may lead them to be sensitive to apoptosis when cells are in oxidative stress. Whereas, the up-regulation of FasL may render astrocytoma cells cytotoxic to neighboring brain tissues and infiltrating immune cells.
authors.affiliation: Department of Microbiology, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemoon-gu, Seoul 120-752, South Korea.
authors.forename: D
authors.initials: D
authors.lastname: Kwon
authors.forename: C
authors.initials: C
authors.lastname: Choi
authors.forename: J
authors.initials: J
authors.lastname: Lee
authors.forename: K O
authors.initials: KO
authors.lastname: Kim
authors.forename: J D
authors.initials: JD
authors.lastname: Kim
authors.forename: S J
authors.initials: SJ
authors.lastname: Kim
authors.forename: I H
authors.initials: IH
authors.lastname: Choi
mesh_terms: D017209:Apoptosis / Q000187:drug effects* / Q000276:immunology; D001254:Astrocytoma; D053222:Fas Ligand Protein; D015870:Gene Expression / Q000187:drug effects / Q000276:immunology; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008562:Membrane Glycoproteins / Q000235:genetics*; D016877:Oxidants / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D019014:fas Receptor / Q000235:genetics*
chemical_list: D053222:Fas Ligand Protein; D008562:Membrane Glycoproteins; D016877:Oxidants; D019014:fas Receptor; D006861:Hydrogen Peroxide