Evolutionary conservation of kinetochore protein sequences in plants.
The evolutionary conservation of structural/functional kinetochore proteins has been studied on isolated nuclei and pro-/metaphase chromosomes of mono- and dicot plants. The cross-reactivities of antibodies against human CENPC, CENPE and CENPF, and against maize CENPCa with the centromeric regions of mitotic chromosomes of Vicia faba and/or Hordeum vulgare are shown. Putative homologs of the kinetochore protein SKP1 (suppressor of kinetochore protein 1p of yeast) were found in both species and of CBF5p (centromere binding factor 5 of yeast) in barley. Antibodies against synthetic peptides derived from partial sequences encoding these proteins were produced and recognized the centromeric regions on mitotic chromosomes as detected by indirect immunofluorescence.
ten Hoopen
R
R
Institute of Plant Genetics and Crop Plant Research (IPK), 06466 Gatersleben, Germany. hoopen@ipk-gatersleben.de
Manteuffel
R
R
Dolezel
J
J
Malysheva
L
L
Schubert
I
I
D000595:Amino Acid Sequence; D001483:Base Sequence; D017931:DNA Primers; D018076:DNA, Complementary; D019143:Evolution, Molecular; D001467:Hordeum / Q000235:genetics; D018386:Kinetochores / Q000378:metabolism*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D003313:Zea mays / Q000235:genetics
D017931:DNA Primers; D018076:DNA, Complementary; D010940:Plant Proteins