title: A 33-35 kDa circulating antigen from Plasmodium falciparum.
abstract: Isolation and characterisation of Plasmodium falciparum (Welch, 1897) soluble antigens from infected patient plasma, Western blotting, thermal stability and ELISA assays using hyperimmune IgG-antimalaria antibodies was the main objective of this work. A circulating antigen of approximately Mr 33-35 kDa with good specificity and antigenicity, in the plasma of malarial patients was shown. Heating at 100 degrees C did not destroy its antigenicity. When fractions highly enriched in the 33-35 kDa proteins were used in ELISAs, a seroreactivity in plasma obtained from primary-infected individuals was found. Controls from normal patients were always negative. The antigenic characteristics suggest that it may be included within the group of new described Plasmodium soluble antigens.
authors.affiliation: Faculty of Pharmacy, Universidad Central de Venezuela, Ciudad Universitaria, Caracas.
authors.forename: N
authors.initials: N
authors.lastname: de Dominguez
authors.forename: A
authors.initials: A
authors.lastname: Rodriguez-Acosta
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D000953:Antigens, Protozoan / Q000097:blood* / Q000276:immunology; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006358:Hot Temperature; D006801:Humans; D007106:Immune Sera; D016778:Malaria, Falciparum / Q000276:immunology / Q000469:parasitology*; D010963:Plasmodium falciparum / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification*; D011817:Rabbits
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007106:Immune Sera