title: Extragenic bypass suppressors of mutations in the essential gene BLD2 promote assembly of basal bodies with abnormal microtubules in Chlamydomonas reinhardtii.
abstract: bld2-1 mutant Chlamydomonas reinhardtii strains assemble basal bodies with singlet microtubules; bld2-1 cells display flagellar assembly defects as well as positioning defects of the mitotic spindle and cleavage furrow. To further understand the role of the BLD2 gene, we have isolated three new bld2 alleles and three partially dominant extragenic suppressors, rgn1-1, rgn1-2, and rgn1-3. bld2 rgn1-1 strains have phenotypes intermediate between those of bld2 and wild-type strains with respect to flagellar number, microtubule rootlet organization, cleavage furrow positioning, and basal body structural phenotypes. Instead of the triplet microtubules of wild-type cells, bld2 rgn1-1 basal bodies have mixtures of no, singlet, doublet, and triplet microtubules. The bld2-4 allele was made by insertional mutagenesis and identified in a noncomplementation screen in a diploid strain. The bld2-4 allele has a lethal phenotype based on mitotic segregation in diploid strains and in haploid strains generated by meiotic recombination. The lethal phenotype in haploid strains is suppressed by rgn1-1; these suppressed strains have similar phenotypes to other bld2 rgn1-1 double mutants. It is likely that BLD2 is an essential gene that is needed for basal body assembly and function.
authors.affiliation: Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80309-0347, USA.
authors.forename: A M
authors.initials: AM
authors.lastname: Preble
authors.forename: T H
authors.initials: TH
authors.lastname: Giddings
authors.forename: S K
authors.initials: SK
authors.lastname: Dutcher
mesh_terms: D000483:Alleles; D000818:Animals; D016825:Chlamydomonas reinhardtii / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure*; D003433:Crosses, Genetic; D004171:Diploidy; D005407:Flagella / Q000235:genetics / Q000648:ultrastructure; D017125:Genes, Protozoan; D005816:Genetic Complementation Test; D006238:Haploidy; D008540:Meiosis / Q000235:genetics; D008854:Microscopy, Electron; D008870:Microtubules / Q000235:genetics* / Q000648:ultrastructure*; D009154:Mutation; D010641:Phenotype; D013489:Suppression, Genetic