Stanniocalcin gene expression during mouse urogenital development: a possible role in mesenchymal-epithelial signalling.
Stanniocalcin (STC) is a polypeptide hormone first discovered in fish and more recently in mammals. In mammals, the STC gene is widely expressed and the hormone is involved in a variety of functions, but STC does not normally circulate in the blood. In both kidney and gut, STC regulates phosphate fluxes across the transporting epithelia, whereas in brain it protects neurons against cerebral ischemia and promotes neuronal cell differentiation. However, the gene is most highly expressed in ovary and expression is dramatically up-regulated by both pregnancy and nursing. STC mRNA levels are also high in the developing mouse embryo, but literally nothing is known of the tissue pattern of gene expression. Therefore, the aim of this study was to map the temporal and spatial patterns of gene expression during mouse embryologic development, starting with the urogenital system where the gene is so highly expressed in adults. STC mRNA was evident as early as E10.5 in both the mesonephros and genital ridge. Between E10.5 and 14.5 in developing kidney, STC was produced in undifferentiated mesenchyme cells and sequestered by ureteric bud epithelial cells that did not express the gene but nonetheless contained high levels of STC protein. Thereafter, the distribution pattern resembled that in adults such that gene expression predominated in collecting duct cells, whereas protein was present in most nephron segments. The pattern of gene expression during gonadal development was sexually dimorphic. In males, expression was first evident on E12.5 in interstitial mesenchyme cells surrounding the developing sex cords, whereas the protein accumulated in developing gonocytes within the sex cords that did not express the gene. This pattern became more pronounced over the course of gestation. In contrast, ovarian gene expression was only weakly evident during development. Collectively, the evidence suggests that in addition to its regulatory effects in adults, STC has novel and distinctive roles in the mesenchymal-epithelial interactions that are vital to normal organogenesis.
Stasko
S E
SE
Department of Physiology, Faculty of Medicine and Dentistry, University of Western Ontario, London, Ontario, Canada.
Wagner
G F
GF
D000818:Animals; D015152:Blotting, Northern; D004848:Epithelium / Q000378:metabolism*; D005260:Female; D006023:Glycoproteins / Q000096:biosynthesis*; D006728:Hormones / Q000096:biosynthesis*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007668:Kidney / Q000196:embryology; D008297:Male; D008648:Mesoderm / Q000378:metabolism*; D051379:Mice; D010053:Ovary / Q000196:embryology; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction; D013737:Testis / Q000196:embryology; D013997:Time Factors; D014018:Tissue Distribution; D015854:Up-Regulation; D014566:Urogenital System / Q000196:embryology*
D006023:Glycoproteins; D006728:Hormones; D012333:RNA, Messenger; C051795:teleocalcin