Expression of tumor necrosis factor alpha and interleukin 1 beta in jejuna of volunteers after experimental challenge with Cryptosporidium parvum correlates with exposure but not with symptoms.
Jejunal biopsies from volunteers challenged with Cryptosporidium parvum were examined for tumor necrosis factor alpha (TNF-alpha) and interleukin (IL)-1 beta mRNA. Postchallenge biopsies from 15 of 28 (54%) volunteers expressed TNF-alpha; 14% expressed IL-1 beta. Cytokine expression did not correlate with enteric symptoms, suggesting that TNF-alpha and IL-1 beta are not key mediators of diarrhea in human cryptosporidiosis.
Robinson
P
P
Department of Medicine, Baylor College of Medicine, Houston, Texas 77030, USA.
Okhuysen
P C
PC
Chappell
C L
CL
Lewis
D E
DE
Shahab
I
I
Janecki
A
A
White
A C
AC
D000818:Animals; D001706:Biopsy; D003457:Cryptosporidiosis / Q000276:immunology*; D016785:Cryptosporidium parvum; D003967:Diarrhea / Q000209:etiology*; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000235:genetics; D019409:Interleukin-15 / Q000096:biosynthesis; D007583:Jejunum / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000235:genetics
D007375:Interleukin-1; D019409:Interleukin-15; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha