Erythromycin modulates eosinophil chemotactic cytokine production by human lung fibroblasts in vitro.
Recent studies suggest that erythromycin can suppress the production of some cytokines and may be an effective treatment for asthma. Eosinophil chemotactic cytokines have been suggested to contribute to the pathogenesis of asthma by the recruitment of eosinophils. We hypothesized that erythromycin modulates eosinophil chemotactic cytokine production. To test the hypothesis, we evaluated the potential of erythromycin to modulate the release of eosinophil chemoattractants from the human lung fibroblast cell line HFL-1. HFL-1 released eotaxin, granulocyte-macrophage colony-stimulating factor, and regulated and normal T-cell expressed and presumably secreted (RANTES) in response to interleukin-1beta or tumor necrosis factor alpha. Erythromycin attenuated the release of these cytokines and eosinophil chemotactic activity by the HFL-1. The suppressive effect on eotaxin was the most marked of these cytokines. Erythromycin therapy also suppressed eotaxin mRNA significantly. These results suggest a mechanism that may account for the apparent beneficial action of macrolide antibiotics in the treatment of allergic airway disorders.
Sato
E
E
Research Service, Southern Arizona Veterans Health Care System, and the Department of Medicine, University of Arizona, Tucson, Arizona 85723, USA.
Nelson
D K
DK
Koyama
S
S
Hoyt
J C
JC
Robbins
R A
RA
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002460:Cell Line; D054413:Chemokine CCL11; D019742:Chemokines, CC; D002631:Chemotactic Factors, Eosinophil / Q000378:metabolism*; D016207:Cytokines / Q000096:biosynthesis* / Q000378:metabolism; D004804:Eosinophils / Q000187:drug effects / Q000378:metabolism*; D004917:Erythromycin / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D006801:Humans; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism
D000900:Anti-Bacterial Agents; C515456:CCL11 protein, human; D054413:Chemokine CCL11; D019742:Chemokines, CC; D002631:Chemotactic Factors, Eosinophil; D016207:Cytokines; D012333:RNA, Messenger; D004917:Erythromycin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor