title: Presence of immunoreactive adrenomedullin in human and bovine milk.
abstract: We examined by radioimmunoassay the presence of immunoreactive adrenomedullin (ir-AM) in human and bovine milk. Milk samples displaced (125)I-AM from the AM-antiserum in parallel to the standard curve. RP-HPLC revealed a main immunoreactive peak eluting as synthetic AM. Concentrations in human milk ranged between 140 and 404 pg/mL. In cow, the levels of AM were 73.5 +/- 3.8 pg/mL. Bovine milk products had AM levels similar to those found in fresh bovine milk. Human milk had growth promoting activity on the human intestinal cell line Int-407 that could be partially blocked with an anti-AM antibody.
authors.affiliation: Department of Cell and Cancer Biology, National Cancer Institute, NIH, Building 10 Room 12N226, 9000 Rockville Pike, Bethesda, MD 20892, USA. pior@mail.nih.gov
authors.forename: R
authors.initials: R
authors.lastname: Pio
authors.forename: A
authors.initials: A
authors.lastname: Martínez
authors.forename: T H
authors.initials: TH
authors.lastname: Elsasser
authors.forename: F
authors.initials: F
authors.lastname: Cuttitta
mesh_terms: D053607:Adrenomedullin; D000818:Animals; D001667:Binding, Competitive; D002417:Cattle; D002455:Cell Division; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007422:Intestines / Q000166:cytology; D008892:Milk / Q000737:chemistry / Q000378:metabolism; D010455:Peptides / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism*; D011863:Radioimmunoassay; D013997:Time Factors
chemical_list: D010455:Peptides; D053607:Adrenomedullin