title: Depletion of moxidectin tissue residues in sheep.
abstract: The pattern of tissue depletion of moxidectin (MXD) subcutaneously administered to sheep was characterized in this study. MXD concentration profiles were determined in muscle, fat, and liver and at the site of injection following administration of a formulation combining MXD (0.5% w/v) with a standard 6 in 1 clostridial vaccine. Thirty-five (35) parasite-free Lincoln sheep were treated with the MXD injectable formulation at a dose rate of 0.2 mg of MXD/kg of live weight, administered subcutaneously on the inner surface of the thigh. Treated animals were sacrificed in randomly selected groups of six sheep weekly from day 21 until day 49 post-treatment. Three nontreated animals were sacrificed to obtain blank tissue samples to validate the analytical methodology. MXD concentration profiles were determined by a validated HPLC analytical method using fluorescence detection. MXD has an adequate pattern of absorption, based on the low residual concentrations found in the injection site area at all sampling intervals. Muscle samples showed the lowest MXD concentrations throughout the study period. The highest MXD concentrations at all sampling times were measured in the adipose tissue, indicating that fat is a target tissue for MXD. MXD concentrations in all of the tissues analyzed were below the accepted maximum limit of residue at 21 days post-treatment.
authors.affiliation: Laboratorio de Farmacologia, Dpto. de Fisiopatologia, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro, Campus Universitario, 7000 Tandil, Argentina.
authors.forename: A
authors.initials: A
authors.lastname: Lifschitz
authors.forename: F
authors.initials: F
authors.lastname: Imperiale
authors.forename: G
authors.initials: G
authors.lastname: Virkel
authors.forename: M
authors.initials: M
authors.lastname: Muñoz Cobeñas
authors.forename: N
authors.initials: N
authors.lastname: Scherling
authors.forename: R
authors.initials: R
authors.lastname: DeLay
authors.forename: C
authors.initials: C
authors.lastname: Lanusse
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry; D000818:Animals; D000900:Anti-Bacterial Agents / Q000493:pharmacokinetics*; D000969:Antinematodal Agents / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid; D004350:Drug Residues / Q000032:analysis*; D007279:Injections, Subcutaneous; D018942:Macrolides; D012756:Sheep / Q000378:metabolism*; D013997:Time Factors; D014018:Tissue Distribution
chemical_list: D000900:Anti-Bacterial Agents; D000969:Antinematodal Agents; D018942:Macrolides; C027837:moxidectin