Hydrogen peroxide triggers the expression of Fas/FasL in astrocytoma cell lines and augments apoptosis.
Fas expression increases in perinecrotic areas of glioblastoma. In this study the up-regulation of Fas/FasL by oxidative stress was shown. H(2)O(2) exposure increased Fas expression in two astrocytoma cell lines and cells became sensitive to agonistic anti-Fas antibody. FasL was up-regulated in astrocytoma cells. Apoptosis of Molt-4 cells was augmented by astrocytoma cells pretreated with H(2)O(2). Our findings suggest up-regulation of Fas in astrocytoma cells may lead them to be sensitive to apoptosis when cells are in oxidative stress. Whereas, the up-regulation of FasL may render astrocytoma cells cytotoxic to neighboring brain tissues and infiltrating immune cells.
Kwon
D
D
Department of Microbiology, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemoon-gu, Seoul 120-752, South Korea.
Choi
C
C
Lee
J
J
Kim
K O
KO
Kim
J D
JD
Kim
S J
SJ
Choi
I H
IH
D017209:Apoptosis / Q000187:drug effects* / Q000276:immunology; D001254:Astrocytoma; D053222:Fas Ligand Protein; D015870:Gene Expression / Q000187:drug effects / Q000276:immunology; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008562:Membrane Glycoproteins / Q000235:genetics*; D016877:Oxidants / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D019014:fas Receptor / Q000235:genetics*
D053222:Fas Ligand Protein; D008562:Membrane Glycoproteins; D016877:Oxidants; D019014:fas Receptor; D006861:Hydrogen Peroxide