title: Wild-type circadian rhythmicity is dependent on closely spaced E boxes in the Drosophila timeless promoter.
abstract: Transcriptional regulation plays an important role in Drosophila melanogaster circadian rhythms. The period promoter has been well studied, but the timeless promoter has not been analyzed in detail. Mutagenesis of the canonical E box in the timeless promoter reduces but does not eliminate timeless mRNA cycling or locomotor activity rhythms. This is because there are at least two other cis-acting elements close to the canonical E box, which can also be transactivated by the circadian transcription factor dCLOCK. These E-box-like sequences cooperate with the canonical E-box element to promote high-amplitude transcription, which is necessary for wild-type rhythmicity.
authors.affiliation: Department of Biology, National Science Foundation Center for Biological Timing, Waltham, Massachusetts 02454, USA.
authors.forename: M J
authors.initials: MJ
authors.lastname: McDonald
authors.forename: M
authors.initials: M
authors.lastname: Rosbash
authors.forename: P
authors.initials: P
authors.lastname: Emery
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D002940:Circadian Rhythm / Q000235:genetics* / Q000502:physiology; D004247:DNA / Q000235:genetics; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics* / Q000502:physiology; D017344:Genes, Insect; D019476:Insect Proteins / Q000235:genetics*; D007438:Introns; D008954:Models, Biological; D009154:Mutation; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D054401:Receptors, CCR8; D019707:Receptors, Chemokine / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics; D014157:Transcription Factors; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; C103330:CCR8 protein, human; D029721:Drosophila Proteins; D019476:Insect Proteins; D012333:RNA, Messenger; D054401:Receptors, CCR8; D019707:Receptors, Chemokine; D012097:Repressor Proteins; D014157:Transcription Factors; C122138:peroxide repressor proteins; C096445:tim protein, Drosophila; D004247:DNA