title: Labeling and distribution of linear peptides identified using in vivo phage display selection for tumors.
abstract: To develop targeting molecules to be used for vascular targeting of short half-lived alpha-emitters for radioimmunotherapy, linear peptide phage display libraries were selected in vivo for binding to IC-12 rat tracheal tumors growing in severe combined immune deficient mice. After three rounds of selection, 15 phage clones were analyzed for DNA sequence, and the deduced translation products of cDNA inserts were compared. Three consensus sequences were chosen from three separate experimental selection series and peptides of these sequences with added -gly-gly-tyr were obtained. Peptides were radiolabeled on tyrosine with (125)I and the biodistribution in tumor-bearing mice was determined. The radioiodinated peptides were stable in vitro and when injected in tumor-bearing mice approximately 3.0 %ID/g accumulated in the tumor; however, much of the (125)I was found in the gastrointestinal tract and thyroid, indicative of dehalogenation of the labeled peptide. Radiolabeling peptide 2 with N-succinimidyl-3-(125)I-iodobenzoate resulted in faster excretion, which in turn resulted in lower levels in tumor and other organs, especially thyroid and gastrointestinal tract. Peptide 2 was derivatized with the bifunctional isothiocyanates of cyclohexyl-B diethylenetriaminepentaacetic acid (DTPA) or CHX-A" DTPA by direct conjugation or with a hydroxylamine derivative of 1B4M-DTPA (2-(p-[O-(carboxamylmethyl)hydroxylamine]benzyl)-6-methyl-diethylenetriamine-N,N,N',N",N"-pentaacetic acid ) coupled at the N-terminus. The primary molecular species in the conjugated products were shown by mass spectrometry to have one DTPA per peptide. Peptide chelate conjugates were radiolabeled with (213)Bi and the products tested for biodistribution in tumor-bearing mice. The data show that chelation of (213)Bi to peptides was accomplished by both the direct method of DTPA attachment and by the method using the linker at the N-terminus. Only small amounts of peptide accumulated at tumor sites. We conclude that phage display is a powerful tool to select peptides with restricted binding specificity; however, the peptides isolated to date do not bind with high retention to tumor sites in vivo.
authors.affiliation: Division of Life Sciences, Oak Ridge, Tennessee 37831-6101, USA. sj9@ornl.gov
authors.forename: S J
authors.initials: SJ
authors.lastname: Kennel
authors.forename: S
authors.initials: S
authors.lastname: Mirzadeh
authors.forename: G B
authors.initials: GB
authors.lastname: Hurst
authors.forename: L J
authors.initials: LJ
authors.lastname: Foote
authors.forename: T K
authors.initials: TK
authors.lastname: Lankford
authors.forename: K A
authors.initials: KA
authors.lastname: Glowienka
authors.forename: L L
authors.initials: LL
authors.lastname: Chappell
authors.forename: J R
authors.initials: JR
authors.lastname: Kelso
authors.forename: S M
authors.initials: SM
authors.lastname: Davern
authors.forename: A
authors.initials: A
authors.lastname: Safavy
authors.forename: M W
authors.initials: MW
authors.lastname: Brechbiel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017104:Bacteriophage M13 / Q000235:genetics*; D001667:Binding, Competitive; D001729:Bismuth; D002614:Chelating Agents / Q000737:chemistry / Q000493:pharmacokinetics; D016384:Consensus Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006898:Hydroxylamines; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D051379:Mice; D016513:Mice, SCID; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism*; D019151:Peptide Library; D010455:Peptides / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics; D011859:Radiography; D016499:Radioimmunotherapy; D011868:Radioisotopes; D014018:Tissue Distribution
chemical_list: D002614:Chelating Agents; D006898:Hydroxylamines; D007457:Iodine Radioisotopes; D019151:Peptide Library; D010455:Peptides; D011868:Radioisotopes; D001729:Bismuth