Flavonoids and phenolic acids of sage: influence of some agricultural factors.
A reversed-phase high-performance liquid chromatography/diode-array detector procedure is proposed for the determination of six phenolic compounds (caffeic acid, luteolin 7-O-glucoside, rosmarinic acid, apigenin, hispidulin, and cirsimaritin) in sage. The chromatographic separation was achieved using a reversed-phase Spherisorb ODS2 (5-microm particle size, 25.0 x 0.46 cm) column. Of the several extractive solvents assayed only ethyl ether, ethyl acetate, and acetone were able to extract all the compounds mentioned. Best resolution was obtained using a gradient of water/phosphoric acid (999:1) and acetonitrile. Ten samples cultivated in two experimental fields (1997-1999) were analyzed and the individual compounds quantified. Four commercially available samples were also analyzed and the results are discussed.
Areias
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CEQUP/Laboratorio de Farmacognosia, Faculdade de Farmacia, Universidade do Porto, R. Anibal Cunha, 4050-047 Porto, Portugal.
Valentão
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Andrade
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PB
Ferreres
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Seabra
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RM
D005419:Flavonoids / Q000032:analysis*; D062385:Hydroxybenzoates / Q000032:analysis*; D019686:Lamiaceae / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D005419:Flavonoids; D062385:Hydroxybenzoates; C017616:phenolic acid