title: Peptide-based targeting of fluorophores to peroxisomes in living cells.
abstract: Peptides carrying different fluorophores can be designed to incorporate spontaneously into living cells when added to the medium. By incorporating the peroxisome-targeting sequence PTS1, the peptide is recognized by the protein-import machinery of peroxisomes and, as a result, can accumulate in these organelles. Depending on the cell type, an inhibitor of the multidrug-resistance protein might be required to ensure strong accumulation. In this update, we discuss the potential of these peptide-linked fluorophores in solving issues related to organelle function and dynamics.
authors.affiliation: Biochemistry of Lipids Dept, Utrecht University, PO Box 80.054, 3508 TB, Utrecht, The Netherlands.
authors.forename: E H
authors.initials: EH
authors.lastname: Pap
authors.forename: T B
authors.initials: TB
authors.lastname: Dansen
authors.forename: K W
authors.initials: KW
authors.lastname: Wirtz
mesh_terms: D000818:Animals; D002463:Cell Membrane Permeability; D018432:Drug Resistance, Multiple; D005456:Fluorescent Dyes / Q000378:metabolism*; D005544:Forecasting; D010455:Peptides / Q000378:metabolism*; D000074435:Peroxisome-Targeting Signal 1 Receptor; D020675:Peroxisomes / Q000378:metabolism*; D021382:Protein Sorting Signals; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D010455:Peptides; D000074435:Peroxisome-Targeting Signal 1 Receptor; D021382:Protein Sorting Signals; D018160:Receptors, Cytoplasmic and Nuclear