title: Neuronal size in the spinal nucleus of the bulbocavernosus: direct modulation by androgen in rats with mosaic androgen insensitivity.
abstract: The motoneurons of the spinal nucleus of the bulbocavernosus (SNB) and its target muscles, the bulbocavernosus and levator ani, form a sexually dimorphic circuit that is developmentally dependent on androgen exposure and exhibits numerous structural and functional changes in response to androgen exposure in adulthood. Castration of male adult rats causes shrinkage of SNB somata, and testosterone replacement reverses this effect, but the site at which androgen is acting to cause this change is undetermined. We exploited the X-chromosome residency of the androgen receptor (AR) gene to generate androgenized female rats that were heterozygous for the testicular feminization mutant (tfm) AR mutation and that, as a consequence of ontogenetic random X-inactivation, expressed a blend of androgen-sensitive wild-type cells and tfm-affected androgen-insensitive cells in the SNB. Chronic testosterone treatment of adult mosaics increased soma sizes only in androgen-competent wild-type SNB cells. The size of tfm-affected SNB somata in the same animals did not differ from the size of either the wild-type or tfm-affected SNB neurons in control mosaics that did not receive androgen treatment in adulthood. Because the muscle targets of the SNB are known to be uniformly androgen-sensitive in tfm mosaics, this mosaic analysis provides unambiguous evidence that androgenic effects on motoneuron soma size are mediated locally in the SNB. It is possible that the neuronal AR plays a permissive role in coordinating the actions of androgen.
authors.affiliation: Department of Psychology, Simon Fraser University, Burnaby, British Columbia, V5A 1S6 Canada. nwatson@sfu.ca
authors.forename: N V
authors.initials: NV
authors.lastname: Watson
authors.forename: L M
authors.initials: LM
authors.lastname: Freeman
authors.forename: S M
authors.initials: SM
authors.lastname: Breedlove
mesh_terms: D013734:Androgen-Insensitivity Syndrome / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D000728:Androgens / Q000008:administration & dosage*; D000818:Animals; D048429:Cell Size / Q000187:drug effects; D004343:Drug Implants; D005260:Female; D008297:Male; D009030:Mosaicism / Q000235:genetics / Q000473:pathology*; D009046:Motor Neurons / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D051381:Rats; D013116:Spinal Cord / Q000187:drug effects / Q000473:pathology*; D013739:Testosterone / Q000008:administration & dosage
chemical_list: D000728:Androgens; D004343:Drug Implants; D013739:Testosterone