title: COX and LOX eicosanoids modulate platelet activation and procoagulation induced by two murine cancer cells.
abstract: Involvement of arachidonic acid cyclooxygenase (COX) and lipoxygenase (LOX) metabolites in platelet aggregation and coagulation induced by two varieties of cancer cells of murine transplantable tumors was studied. A lung alveolar carcinoma (LAC) and a fibrosarcoma (FS), induced platelet aggregation and plasma coagulation (P<0.05). Pretreatment of both tumor lines with a COX inhibitor did not block the tumor cell induced platelet aggregation (TCIPA). COX [12(S)-HTT] and LOX [12(S)-HETE], metabolites of washed platelets (WP), alone or co-incubated with LAC or FS cells, were analyzed. We observed higher 12(S)-HETE release with respect to 12(S)HHT when WP were co-incubated with LAC cells. With both neoplastic cell (NC) lines prothrombin time (PT) was shortened. Pretreatment of NC with iodoacetic acid, soybean trypsin inhibitor or Factor X-deficient plasma increased the PT. These results indicate that AA metabolites play a role on the procoagulation and platelet aggregation induced by mesenchymal and epithelial murine cancers.
authors.affiliation: Departamento de Histología, Embriología y Genética, Instituto de Biología Celular, Facultad de Ciencias, Universidad Nacional de Córdoba, Argentina.
authors.forename: M E
authors.initials: ME
authors.lastname: Pasqualini
authors.forename: C E
authors.initials: CE
authors.lastname: Mohn
authors.forename: J P
authors.initials: JP
authors.lastname: Petiti
authors.forename: P
authors.initials: P
authors.lastname: Manzo
authors.forename: A R
authors.initials: AR
authors.lastname: Eynard
mesh_terms: D002282:Adenocarcinoma, Bronchiolo-Alveolar / Q000378:metabolism / Q000473:pathology*; D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism; D001777:Blood Coagulation / Q000187:drug effects*; D018920:Coculture Techniques; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000378:metabolism*; D015777:Eicosanoids / Q000096:biosynthesis / Q000145:classification / Q000502:physiology*; D005170:Factor X / Q000502:physiology; D005354:Fibrosarcoma / Q000378:metabolism / Q000473:pathology*; D019807:Iodoacetic Acid / Q000494:pharmacology; D008084:Lipoxygenase / Q000378:metabolism*; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009363:Neoplasm Proteins; D015539:Platelet Activation / Q000187:drug effects*; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D019851:Thrombophilia / Q000209:etiology / Q000503:physiopathology; D014360:Trypsin Inhibitor, Kunitz Soybean / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
chemical_list: D001095:Arachidonic Acids; D015777:Eicosanoids; D009363:Neoplasm Proteins; D005170:Factor X; D014360:Trypsin Inhibitor, Kunitz Soybean; D008084:Lipoxygenase; D011451:Prostaglandin-Endoperoxide Synthases; D003546:Cysteine Endopeptidases; C030910:cancer procoagulant; D019807:Iodoacetic Acid