Methods for measuring glutathione concentration and rate of synthesis.
Altered gamma-glutamylcysteinylglycine homeostasis has been implicated in a wide variety of human diseases. The measurement of the rates of synthesis or loss of gamma-glutamylcysteinylglycine is necessary in order to make meaningful inferences about changes in gamma-glutamylcysteinylglycine concentration in these diseased states. In this review, we discuss methods for measuring gamma-glutamylcysteinylglycine concentration in biological samples as well as how improvements in the sensitivity of gas chromatography-mass spectrometric analyses have permitted the development of new and convenient stable isotope tracer methods for the in-vivo measurement of gamma-glutamylcysteinylglycine kinetics.
Reid
M
M
USDA/Agricultural Research Service, Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA.
Jahoor
F
F
D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D005978:Glutathione / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D006801:Humans; D007553:Isotope Labeling; D009682:Magnetic Resonance Spectroscopy; D010084:Oxidation-Reduction; D012680:Sensitivity and Specificity
D005978:Glutathione