Rapid detection of enterohemorrhagic Escherichia coli by real-time PCR with fluorescent hybridization probes.
In this report, we present a PCR protocol for rapid identification of enterohemorrhagic Escherichia coli on a LightCycler instrument. In a multiplex assay, the genes encoding Shiga toxin 1 and Shiga toxin 2 are detected in a single reaction capillary. A complete analysis of up to 32 samples takes about 45 min.
Bellin
T
T
Hannover Medical School, Institute of Medical Microbiology, 30625 Hannover, Germany.
Pulz
M
M
Matussek
A
A
Hempen
H G
HG
Gunzer
F
F
D000818:Animals; D002417:Cattle; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D005456:Fluorescent Dyes; D006801:Humans; D008460:Meat / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D022622:Shiga Toxin 1 / Q000235:genetics; D022641:Shiga Toxin 2 / Q000235:genetics
D015342:DNA Probes; D004269:DNA, Bacterial; D005456:Fluorescent Dyes; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2