Neutrophils, not complement, mediate the mortality of experimental hemorrhagic pancreatitis.
Chemoactivation of the neutrophil (PMN) via the complement system has been observed in many inflammatory conditions and is thought to play a pathogenic role in acute pancreatitis. This study examined the effects of PMN depletion in experimental hemorrhagic pancreatitis and tested the role played by complement. Severe pancreatitis was induced by a choline-deficient, 0.5% ethionine-supplemented diet in female Institute of Cancer Research (ICR) mice weighing 11-13 g. Neutropenia was induced by an antibody injection. Total complement depletion was achieved by tail vein injections of cobra venom factor (CVF). Serum amylase levels and local pancreatic injury were not significantly modulated by either PMN or complement depletion at 72 hours. Systemic and remote organ injury, assessed by the formation of ascites, hematocrit, and serum alanine aminotransferase levels, was significantly reduced in neutropenic mice but failed to be moderated by complement depletion. In addition, liver and lung myeloperoxidase activity was independent of complement depletion. At 5 days, mortality was zero in PMN-depleted mice. There was no improvement in survival in the CVF-treated group. Neutrophils are important in the systemic injury and mortality of severe pancreatitis. PMN chemoactivation involves mechanisms other than complement.
Kyriakides
C
C
Department of Surgery, Brigham and Women's Hospital, and Harvard Medical School, Boston, Massachusetts 02115, USA.
Jasleen
J
J
Wang
Y
Y
Moore
F D
FD
Ashley
S W
SW
Hechtman
H B
HB
D000208:Acute Disease; D000410:Alanine Transaminase / Q000378:metabolism; D000681:Amylases / Q000097:blood; D000818:Animals; D003167:Complement Activation / Q000502:physiology; D003165:Complement System Proteins / Q000502:physiology*; D005260:Female; D006470:Hemorrhage / Q000209:etiology / Q000401:mortality* / Q000503:physiopathology; D051379:Mice; D008813:Mice, Inbred ICR; D009503:Neutropenia / Q000150:complications; D018375:Neutrophil Activation / Q000502:physiology; D009504:Neutrophils / Q000502:physiology*; D010195:Pancreatitis / Q000209:etiology / Q000401:mortality* / Q000503:physiopathology; D009195:Peroxidase / Q000378:metabolism
D003165:Complement System Proteins; D009195:Peroxidase; D000410:Alanine Transaminase; D000681:Amylases