BM-40 and MMP-2 expression are not coregulated in human melanoma cell lines.
As published recently, suppression of BM-40 (osteonectin, SPARC) expression reduced the in vitro adhesive and invasive capacities of melanoma cells. In parallel, a decreased production of matrix metalloproteinase-2 (MMP-2) was observed in these cells indicating a close coregulation of these two proteins. However, analysis of 12 independently established melanoma cell lines, exhibiting different metastatic capacities, did not reveal a positive correlation between BM-40 or MMP-2 expression and the metastatic capacities of these cell lines. In addition, direct comparison of BM-40 and MMP-2 mRNA levels did not provide evidence for coregulation of these two proteins. This was further corroborated by the finding that exogenous BM-40 failed to alter synthesis and activation of proMMP-2.
Nischt
R
R
Department of Dermatology, University of Cologne, D-50924, Cologne, Germany. roswitha.nischt@uni-koeln.de
Wallich
M
M
Reibetanz
M
M
Baumann
P
P
Krieg
T
T
Mauch
C
C
D017364:Collagenases / Q000378:metabolism; D004789:Enzyme Activation; D004792:Enzyme Precursors / Q000378:metabolism; D018093:Gelatinases / Q000378:metabolism; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D020780:Matrix Metalloproteinase 9; D008545:Melanoma / Q000201:enzymology / Q000378:metabolism* / Q000556:secondary; D008666:Metalloendopeptidases / Q000378:metabolism; D009362:Neoplasm Metastasis; D015676:Osteonectin / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014407:Tumor Cells, Cultured
D004792:Enzyme Precursors; D015676:Osteonectin; D012333:RNA, Messenger; D017364:Collagenases; D018093:Gelatinases; D008666:Metalloendopeptidases; C120487:pro-matrix metalloproteinase 9; C069860:progelatinase; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9