Direct effects of corticotropin-releasing hormone and thyrotropin-releasing hormone on fetal lung explants.
Fetal lung produces corticotropin-releasing hormone (CRH) without known direct effects. We tested the hypothesis that CRH can directly regulate lung development. In baboon fetal lung explants, CRH strongly induces surfactant phospholipid synthesis and SP-C immunostaining, plus [(3)H]thymidine incorporation. CRH receptor mRNA was detected in lung from multiple baboons at e125. Testing thyrotropin (TRH) as a specificity control, we did demonstrate different direct effects with only modest stimulation of surfactant phospholipid synthesis and strong induction of cytidylyltransferase gene expression. Therefore, CRH, similar to ACTH and glucocorticoids, is a potent inducer of cell differentiation in fetal lung.
Emanuel
R L
RL
Department of Medicine, Children's Hospital & Harvard Medical School, Boston, MA 02115, USA.
Torday
J S
JS
Asokananthan
N
N
Sunday
M E
ME
D000367:Age Factors; D000818:Animals; D001839:Bombesin / Q000494:pharmacology; D002454:Cell Differentiation; D002794:Choline / Q000378:metabolism; D019916:Choline-Phosphate Cytidylyltransferase / Q000378:metabolism; D003346:Corticotropin-Releasing Hormone / Q000494:pharmacology*; D003907:Dexamethasone / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005865:Gestational Age; D008168:Lung / Q000187:drug effects / Q000196:embryology*; D009924:Organ Culture Techniques; D010215:Papio; D009195:Peroxidase / Q000378:metabolism; D010743:Phospholipids / Q000096:biosynthesis; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013501:Surface-Active Agents / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D013973:Thyrotropin-Releasing Hormone / Q000494:pharmacology*; D013997:Time Factors
D010743:Phospholipids; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger; D013501:Surface-Active Agents; D013973:Thyrotropin-Releasing Hormone; D003907:Dexamethasone; D003346:Corticotropin-Releasing Hormone; D009195:Peroxidase; D019916:Choline-Phosphate Cytidylyltransferase; D002794:Choline; D001839:Bombesin; D013936:Thymidine