LFA-1 expression on target cells promotes human immunodeficiency virus type 1 infection and transmission.
While CD4 and the chemokine receptors are the principal receptors for human immunodeficiency virus (HIV), other cellular proteins, such as LFA-1, are also involved in HIV infection. LFA-1 and its ligands, ICAM-1, ICAM-2, and ICAM-3, can be expressed on the cells infected by HIV, as well as on the HIV virions themselves. To examine the role of LFA-1 expressed on target cells in HIV infection, Jurkat-derived Jbeta2.7 T-cell lines that express either wild-type LFA-1, a constitutively active mutant LFA-1, or no LFA-1 were used. The presence of wild-type LFA-1 enhanced the initial processes of HIV infection, as well as the subsequent replication and transmission from cell to cell. In contrast, the constitutively active LFA-1 mutant failed to promote virus replication and spread, even though this mutant could help HIV enter cells and establish the initial infection. This study clearly demonstrates the contribution of LFA-1 in the different stages of HIV infection. Moreover, not only is LFA-1 expression important for initial HIV-cell interaction, subsequent replication, and transmission, but its activity must also be properly regulated.
Hioe
C E
CE
New York University School of Medicine and Manhattan VA Medical Center, New York, New York 10010, USA. hioec01@med.nyu.edu
Chien
P C
PC
Lu
C
C
Springer
T A
TA
Wang
X H
XH
Bandres
J
J
Tuen
M
M
D002448:Cell Adhesion; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D019169:Jurkat Cells; D008024:Ligands; D016169:Lymphocyte Function-Associated Antigen-1 / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D009154:Mutation; D013601:T-Lymphocytes / Q000378:metabolism* / Q000821:virology*; D014779:Virus Replication
D008024:Ligands; D016169:Lymphocyte Function-Associated Antigen-1