title: Genomic exploration of the hemiascomycetous yeasts: 12. Kluyveromyces marxianus var. marxianus.
abstract: As part of the comparative genomics project 'GENOLEVURES', we studied the Kluyveromyces marxianus var. marxianus strain CBS712 using a partial random sequencing strategy. With a 0.2 x genome equivalent coverage, we identified ca. 1300 novel genes encoding proteins, some containing spliceosomal introns with consensus splice sites identical to those of Saccharomyces cerevisiae, 28 tRNA genes, the whole rDNA repeat, and retrotransposons of the Ty1/2 family of S. cerevisiae with diverged Long Terminal Repeats. Functional classification of the K. marxianus genes, as well as the analysis of the paralogous gene families revealed few differences with respect to S. cerevisiae. Only 42 K. marxianus identified genes are without detectable homolog in the baker's yeast. However, we identified several genetic rearrangements between these two yeast species.
authors.affiliation: Unité de Génétique Moléculaire des Levures (URA 2171 du CNRS, UFR 927 Université Pierre et Marie Curie), Institut Pasteur, Paris, France. llorente@pasteur.fr
authors.forename: B
authors.initials: B
authors.lastname: Llorente
authors.forename: A
authors.initials: A
authors.lastname: Malpertuy
authors.forename: G
authors.initials: G
authors.lastname: Blandin
authors.forename: F
authors.initials: F
authors.lastname: Artiguenave
authors.forename: P
authors.initials: P
authors.lastname: Wincker
authors.forename: B
authors.initials: B
authors.lastname: Dujon
mesh_terms: D001203:Ascomycota / Q000235:genetics; D001483:Base Sequence; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D003062:Codon; D017124:Conserved Sequence; D020451:Contig Mapping; D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D005656:Fungal Proteins / Q000145:classification / Q000235:genetics; D005815:Genetic Code; D016681:Genome, Fungal; D007438:Introns; D007716:Kluyveromyces / Q000235:genetics*; D005810:Multigene Family; D009687:Nuclear Proteins / Q000235:genetics; D016366:Open Reading Frames; D012343:RNA, Transfer; D018626:Retroelements; D017381:Spliceosomes
chemical_list: D003062:Codon; D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D005656:Fungal Proteins; D009687:Nuclear Proteins; D018626:Retroelements; D012343:RNA, Transfer