Radiation induced effect of the vitamins C, E and beta-carotene on sanazole efficiency. A study in vitro.
The effect of the vitamins C, E-acetate and beta-carotene on the sanazole (AK-2123) efficiency was investigated in vitro (E. coli bacteria AB 1157) under the influence of gamma-rays. The vitamins were applied with sanazole individually or in appropriate mixtures in airfree media as well as in media saturated with nitrous oxide (N2O) or air. The addition of vitamin C led in all cases to a very strong enhancement of the sanazole efficiency, namely: in airfree media to 2-fold, in the presence of N2O to 1.5-fold and in the presence of air to 2-fold. The influence of vitamin E-acetate and beta-carotene and mixtures of them depended on the respective gas used for saturation of the medium. An attempt was made to estimate the role of the reducing (e-aq, H-atoms) and oxidizing radicals (OH, O2.-) in respect to the influence of the sanazole activity. The results are of interest for the chemoradiation therapy of cancer.
Heinrich
E
E
Ludwig Boltzmann Institute for Radiation Chemistry and Radiation Biology, University of Vienna, Althanstr. 14, A-1090 Vienna, Austria.
Getoff
N
N
D000388:Air; D001205:Ascorbic Acid / Q000494:pharmacology*; D004307:Dose-Response Relationship, Radiation; D004357:Drug Synergism; D004926:Escherichia coli / Q000187:drug effects / Q000528:radiation effects; D017665:Hydroxyl Radical / Q000737:chemistry; D009609:Nitrous Oxide / Q000737:chemistry; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D014230:Triazoles / Q000494:pharmacology*; D014810:Vitamin E / Q000494:pharmacology*; D019207:beta Carotene / Q000494:pharmacology*
D011838:Radiation-Sensitizing Agents; D014230:Triazoles; D019207:beta Carotene; C072690:AK 2123; D014810:Vitamin E; D017665:Hydroxyl Radical; D009609:Nitrous Oxide; D001205:Ascorbic Acid