Small-angle neutron scattering study of the n-decane effect on the bilayer thickness in extruded unilamellar dioleoylphosphatidylcholine liposomes.
Dioleoylphosphatidylcholine (DOPC) and n-decane were mixed and hydrated afterwards in an excess of heavy water at 1 wt.% of DOPC. From this dispersion, unilamellar liposomes were prepared by extrusion through polycarbonate filter with 500-A pores. Small-angle neutron scattering (SANS) was conducted on these liposomes. From the Kratky-Porod plot ln[I(Q)Q2] vs. Q2 of SANS intensity I(Q) in the range of scattering vectors Q corresponding to the interval 0.001 A(-2) < or = Q2 < or = 0.006 A(-2), the liposome bilayer radius of gyration Rg and the bilayer thickness parameter d(g) = 12(0.5)Rg were obtained. The values of d(g) indicated that the bilayer thickness is within the experimental error constant up to n-decane/DOPC approximately 0.5 molar ratio, and then increases by 2.4 +/- 1.3 A up to n-decane/DOPC = 1.2 molar ratio.
Uhríková
D
D
Faculty of Pharmacy, J.A. Comenius University, Bratislava, Slovakia.
Balgavý
P
P
Kucerka
N
N
Islamov
A
A
Gordeliy
V
V
Kuklin
A
A
D000473:Alkanes / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D009502:Neutrons; D010713:Phosphatidylcholines / Q000737:chemistry*; D013053:Spectrophotometry / Q000379:methods
D000473:Alkanes; D008051:Lipid Bilayers; D008081:Liposomes; D010713:Phosphatidylcholines; C017251:1,2-oleoylphosphatidylcholine; C012867:decane