The cytokinesis gene KEULE encodes a Sec1 protein that binds the syntaxin KNOLLE.
KEULE is required for cytokinesis in Arabidopsis thaliana. We have positionally cloned the KEULE gene and shown that it encodes a Sec1 protein. KEULE is expressed throughout the plant, yet appears enriched in dividing tissues. Cytokinesis-defective mutant sectors were observed in all somatic tissues upon transformation of wild-type plants with a KEULE-green fluorescent protein gene fusion, suggesting that KEULE is required not only during embryogenesis, but at all stages of the plant's life cycle. KEULE is characteristic of a Sec1 protein in that it appears to exist in two forms: soluble or peripherally associated with membranes. More importantly, KEULE binds the cytokinesis-specific syntaxin KNOLLE. Sec1 proteins are key regulators of vesicle trafficking, capable of integrating a large number of intra- and/or intercellular signals. As a cytokinesis-related Sec1 protein, KEULE appears to represent a novel link between cell cycle progression and the membrane fusion apparatus.
Assaad
F F
FF
Genetics and Microbiology Institute, Ludwig Maximilians University, D-80638 Munich, Germany. fassaad@andrew2.stanford.edu
Huet
Y
Y
Mayer
U
U
Jürgens
G
G
D051339:Actin Depolymerizing Factors; D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology; D029681:Arabidopsis Proteins; D015153:Blotting, Western; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D018797:Cell Cycle Proteins; D002455:Cell Division / Q000235:genetics* / Q000502:physiology; D016386:Chromosome Walking; D003001:Cloning, Molecular; D017343:Genes, Plant; D017930:Genes, Reporter / Q000235:genetics; D008565:Membrane Proteins / Q000378:metabolism*; D008840:Microfilament Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D010944:Plants / Q000033:anatomy & histology / Q000378:metabolism; D021381:Protein Transport / Q000502:physiology; D050765:Qa-SNARE Proteins; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012639:Seeds / Q000648:ultrastructure; D016415:Sequence Alignment
C426230:AT1G12360 protein, Arabidopsis; D051339:Actin Depolymerizing Factors; D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; C097724:KNOLLE protein, Arabidopsis; D008565:Membrane Proteins; D008840:Microfilament Proteins; D010940:Plant Proteins; D050765:Qa-SNARE Proteins; D011993:Recombinant Fusion Proteins