title: Distribution of AAV-TK following intracranial convection-enhanced delivery into rats.
abstract: Adeno-associated virus (AAV)-based vectors are being tested in animal models as viable treatments for glioma and neurodegenerative disease and could potentially be employed to target a variety of central nervous system disorders. The relationship between dose of injected vector and its resulting distribution in brain tissue has not been previously reported nor has the most efficient method of delivery been determined. Here we report that convection-enhanced delivery (CED) of 2.5 x 10(8), 2.5 x 10(9), or 2.5 x 10(10) particles of AAV-thymidine kinase (AAV-TK) into rat brain revealed a clear dose response. In the high-dose group, a volume of 300 mm3 of brain tissue was partially transduced. Results showed that infusion pump and subcutaneous osmotic pumps were both capable of delivering vector via CED and that total particle number was the most important determining factor in obtaining efficient expression. Results further showed differences in histopathology between the delivery groups. While administration of vector using infusion pump had relatively benign effects, the use of osmotic pumps resulted in notable toxicity to the surrounding brain tissue. To determine tissue distribution of vector following intracranial delivery, PCR analysis was performed on tissues from rats that received high doses of AAV-TK. Three weeks following CED, vector could be detected in both hemispheres of the brain, spinal cord, spleen, and kidney.
authors.affiliation: Avigen, Inc., Alameda, CA, USA.
authors.forename: J
authors.initials: J
authors.lastname: Cunningham
authors.forename: Y
authors.initials: Y
authors.lastname: Oiwa
authors.forename: D
authors.initials: D
authors.lastname: Nagy
authors.forename: G
authors.initials: G
authors.lastname: Podsakoff
authors.forename: P
authors.initials: P
authors.lastname: Colosi
authors.forename: K S
authors.initials: KS
authors.lastname: Bankiewicz
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism / Q000473:pathology; D018481:Convection; D000229:Dependovirus / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D018390:Gene Targeting; D005822:Genetic Vectors / Q000633:toxicity; D007091:Image Processing, Computer-Assisted; D007124:Immunoenzyme Techniques; D007260:Infusion Pumps; D009336:Necrosis; D009997:Osmotic Pressure; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013937:Thymidine Kinase / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D014161:Transduction, Genetic; D019076:Transgenes
chemical_list: D012333:RNA, Messenger; D013937:Thymidine Kinase