title: Bovine interferon-tau stimulates the Janus kinase-signal transducer and activator of transcription pathway in bovine endometrial epithelial cells.
abstract: Trophoblastic bovine interferon-tau (bIFN-tau) suppresses luteolytic pulses of endometrial prostaglandin F(2alpha) (PGF(2alpha)) at the time of maternal recognition of pregnancy. This results in maintenance of the corpus luteum in cattle. The hypothesis that effects of bIFN-tau in the endometrium were through activation of the Janus kinase (JAK)-signal transducer and activator of transcription (STAT) pathway of signal transduction was tested. Whole cell, cytosolic, and nuclear extracts from bovine endometrial cells treated with bIFN-tau were analyzed by immunoprecipitation, immunoblotting, and electrophoretic mobility shift assays in a series of dose- and time-dependency experiments. Bovine IFN-tau stimulated tyrosine phosphorylation, homo- and heterodimer formation, nuclear translocation, and DNA binding of STAT proteins 1, 2, and 3. Moreover, bIFN-tau induced synthesis of interferon-regulatory factor. In conclusion, bIFN-tau stimulates the JAK-STAT pathway in the bovine endometrium. It is proposed that activation of the JAK-STAT pathway is involved in regulating the antiluteolytic effects of bIFN-tau.
authors.affiliation: Departments of Dairy and Poultry Sciences, University of Florida, Gainesville, FL 32611, USA.
authors.forename: M
authors.initials: M
authors.lastname: Binelli
authors.forename: P
authors.initials: P
authors.lastname: Subramaniam
authors.forename: T
authors.initials: T
authors.lastname: Diaz
authors.forename: G A
authors.initials: GA
authors.lastname: Johnson
authors.forename: T R
authors.initials: TR
authors.lastname: Hansen
authors.forename: L
authors.initials: L
authors.lastname: Badinga
authors.forename: W W
authors.initials: WW
authors.lastname: Thatcher
mesh_terms: D000818:Animals; D002417:Cattle; D004268:DNA-Binding Proteins / Q000235:genetics*; D019281:Dimerization; D004586:Electrophoresis; D004717:Endometrium / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D004847:Epithelial Cells / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D005260:Female; D015151:Immunoblotting; D007150:Immunohistochemistry; D007370:Interferon Type I / Q000494:pharmacology*; D053613:Janus Kinase 1; D009687:Nuclear Proteins / Q000378:metabolism; D010641:Phenotype; D010766:Phosphorylation; D011233:Precipitin Tests; D011257:Pregnancy Proteins / Q000494:pharmacology*; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D050794:STAT1 Transcription Factor; D015398:Signal Transduction / Q000187:drug effects*; D013997:Time Factors; D015534:Trans-Activators / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D007370:Interferon Type I; D009687:Nuclear Proteins; D011257:Pregnancy Proteins; D050794:STAT1 Transcription Factor; D015534:Trans-Activators; C020947:interferon tau; D011505:Protein-Tyrosine Kinases; D053613:Janus Kinase 1