title: Pathomorphological changes in microcirculation of pancreas during experimental acute pancreatitis.
abstract: OBJECTIVE
  Severe acute pancreatitis is prevalent in Eastern Bohemia (a part of the Czech Republic) and remains a very difficult problem to manage. Recent studies in treatment there are quite frequent but a direct view into the pancreas during its inflammatory process is very rare. Only information about a normal pancreatic microvascular bed appears to be available. This study was designed to explore pathomorphological changes in pancreatic microcirculation at the start and during the development of acute pancreatitis.
  
  
  METHODS
  A group of 50 laboratory white rats was studied. The acute pancreatitis was induced by the modified method of Siech et al. The method of clamping of biliopancreatic duct and stimulation of external secretory tissue by a cholecystokinin and secretin and oral (orogastric tube) ethanol administration was performed. The pancreatic microvascular patterns were observed by using histochemical and corrosion casts methods.
  
  
  RESULTS
  The study of the corrosion casts of pancreatic microcirculation in the scanning electron microscope and histochemical studies demonstrated the visible reduction of the pancreatic microvascular bed 18 hours after induction of acute pancreatitis. The microvascular bed is not fully destroyed until 48 hours of acute pancreatitis.
  
  
  CONCLUSIONS
  The model of acute pancreatitis using postoperative application of ethanol to the digestive tract after stimulation of pancreas by cholecystokinine and secretin in the rats seems to be real and useful. The study of the corrosion casts of microcirculation in the scanning electron microscope and histochemical studies demonstrated the visible reduction of the pancreatic microvascular bed 18 hours after induction of acute pancreatitis. The microvascular bed is not fully destroyed until 48 hours of running acute pancreatitis, as some "islets" of the vital tissue still have undestroyed microvessels at this time. Despite the above-mentioned serious changes, restricted pancreatic microcirculation enables blood and medicament distribution to the still intact pancreatic tissue.
authors.affiliation: Department of Surgery, Charles University, Medical Faculty, Hradec Králové, Czech Republic.
authors.forename: M
authors.initials: M
authors.lastname: Kaska
authors.forename: B
authors.initials: B
authors.lastname: Pospísilová
authors.forename: D
authors.initials: D
authors.lastname: Slízová
mesh_terms: D000208:Acute Disease; D000818:Animals; D002766:Cholecystokinin; D000431:Ethanol; D006651:Histocytochemistry; D008297:Male; D008833:Microcirculation; D023421:Models, Animal; D010179:Pancreas / Q000098:blood supply*; D010195:Pancreatitis / Q000473:pathology*; D051381:Rats; D012633:Secretin
chemical_list: D012633:Secretin; D000431:Ethanol; D002766:Cholecystokinin