Molecular characterization of the iron-hydroxamate uptake system in Staphylococcus aureus.
To investigate iron uptake, a chromosomal locus containing three consecutive open reading frames, designated fhuC, fhuB, and fhuD, was identified in Staphylococcus aureus. Whereas the fhuC gene encodes an ATP-binding protein, fhuB and fhuD code for ferrichrome permeases and thus resemble an ATP-binding cassette transporter. A fhuB knockout mutant showed impaired uptake of iron bound to the siderophores but not of ferric chloride, suggesting that this operon is specific for siderophore-mediated iron uptake.
Cabrera
G
G
Microbiology Group, Department of Biological Sciences, Illinois State University, Normal, Illinois 61790-4120, USA.
Xiong
A
A
Uebel
M
M
Singh
V K
VK
Jayaswal
R K
RK
D018528:ATP-Binding Cassette Transporters; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D003470:Culture Media; D029968:Escherichia coli Proteins; D005290:Ferric Compounds / Q000378:metabolism*; D006877:Hydroxamic Acids / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D009154:Mutation; D009876:Operon; D033902:Periplasmic Binding Proteins; D013211:Staphylococcus aureus / Q000235:genetics* / Q000378:metabolism*
D018528:ATP-Binding Cassette Transporters; D002352:Carrier Proteins; D003470:Culture Media; D029968:Escherichia coli Proteins; D005290:Ferric Compounds; C066629:FhuC protein, E coli; D006877:Hydroxamic Acids; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D033902:Periplasmic Binding Proteins; C074579:iron (III) hydroxamate; C069968:fhuB protein, E coli; C069969:fhuD protein, E coli