Induction of micronuclei formation in preimplantation mouse embryos after maternal treatment with 2-bromopropane.
We examined effects of 2-bromopropane (2-BP), a chlorofluorocarbon replacement, on mouse embryonic mutagenicity. 2-BP was administered to pregnant mice intraperitoneally (i.p.) (300, 600, 900, and 1800 mg/kg) during the early preimplantation period. On day 3 of gestation, micronuclei (MN) frequency and embryo cell number were determined. 2-BP induced a dose-related significant increase in MN frequency and a treatment-related decrease in embryo cell number. Furthermore, the cell numbers were significantly smaller in the MN-positive embryos by two-way ANOVA taking it into account an interactive effect between 2-BP dose and the presence or absence of MN. A simultaneous decrease in cell number and increase in MN frequency may reflect an embryonic developmental disadvantage resulting from maternal treatment with 2-BP. Further study is needed to establish how 2-BP contributes to postimplantation embryonic development.
Ishikawa
H
H
Department of Public Health, Mie University School of Medicine, 2-174, Edobashi, 514-8507, Tsu, Japan. jinishi@doc.medic.mie-u.ac.jp
Tian
Y
Y
Yamauchi
T
T
D000818:Animals; D001755:Blastocyst / Q000187:drug effects* / Q000648:ultrastructure; D005260:Female; D006842:Hydrocarbons, Brominated / Q000633:toxicity*; D051379:Mice; D008813:Mice, Inbred ICR; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects*; D011247:Pregnancy; D012034:Refrigeration
D006842:Hydrocarbons, Brominated; C103736:2-bromopropane