[Inhibitory effect of alpha tocopherol succinate on fibroblast wound healing].
OBJECTIVE
To evaluate and compare the effect of acid tocopherol succinate (ATS) on fibroblast migration and proliferation.


METHODS
In vitro wound repair was determined in confluent fibroblast monolayer. Conjunctival fibroblasts were incubated with serum-deprived medium for 24 hours. After this time an artificial wound was made and the cells were incubated with fresh medium plus the doses of ATS to be tested. The cell free area was monitored at 0, 18, 24 and 48 hours. Groups of treatment: Group 1: ethanol 0.1%. Group 2: ATS 25 microM dissolved in ethanol (final concentration 0.1%) Group 3: ATS 50 microM in ethanol 0.1%. Each experiment was carried out in triplicate and repeated 4 times.


RESULTS
There were no differences among the groups during the first 24 hours. ATS showed significantly larger cell-free area at 48 hours. There were no signs of cellular toxicity.


CONCLUSIONS
25 microM and 50 microM ATS inhibit fibroblast proliferation without cellular toxicity.
Pinilla
I
I
Servicio de Oftalmología, Hospital Miguel Servet, Zaragoza, Unidad Mixta de Investigación, Universidad de Zaragoza, España.
Piazuelo
E
E
Jiménez
P
P
Polo
V
V
Larrosa
J M
JM
Abecia
E
E
Honrubia
F M
FM
D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D024505:Tocopherols; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
D014810:Vitamin E; D024505:Tocopherols