Measurement of trace elements in proteins extracted from liver by size exclusion chromatography-inductively coupled plasma-mass spectrometry with a magnetic sector mass spectrometer.
Proteins are extracted from liver into aqueous buffer at pH 7 and separated by size exclusion chromatography (SEC). Inductively coupled plasma mass spectrometry (ICP-MS) with a magnetic sector mass spectrometer is used to identify those protein fractions that contain Cu, Zn, Mn, Fe, Cd, S, P, Mo, Co, Ca, or Mg. The experimental setup provides very high sensitivity. Measurements at medium spectral resolution remove polyatomic interferences for some difficult elements like Fe, S, and P. Some elements are found in different molecular weight proteins; for example, cadmium binds to four different protein fractions (>400 kDa, 70 kDa, and metallothionein). Other elements like Mo, Ca, and Mg are present only in low-molecular-weight proteins or other small molecules.
Wang
J
J
Department of Chemistry, Iowa State University, Ames, Iowa, 50011, USA.
Dreessen
D
D
Wiederin
D R
DR
Houk
R S
RS
D000818:Animals; D002138:Calibration; D002417:Cattle; D002850:Chromatography, Gel / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000737:chemistry*; D008280:Magnetics; D013058:Mass Spectrometry / Q000379:methods*; D008668:Metallothionein / Q000032:analysis; D008670:Metals / Q000032:analysis*; D008970:Molecular Weight; D009994:Osmolar Concentration; D011506:Proteins / Q000737:chemistry*; D014131:Trace Elements / Q000032:analysis*
D008670:Metals; D011506:Proteins; D014131:Trace Elements; D008668:Metallothionein