Quinolizin-coumarins as physical enhancers of chemiluminescence during lipid peroxidation in live HL-60 cells.
We investigated whether physical enhancers of low-level chemiluminescence-coumarin laser dyes C-314, C-334, and C-525--may be used to monitor interactions of lipid peroxyl radicals during lipid peroxidation in live cells. We present data demonstrating that two quinolizin-substituted coumarins--C-525 and C-334--can be integrated into HL-60 cells and successfully used as physical enhancers of chemiluminescence induced by the lipid soluble azo-initiator 2,2'-azobis(2,4-dimethyl-valeronitrile) (AMVN). Coumarins did not inhibit AMVN-induced peroxidation of membrane phospholipids in HL-60 cells, and no consumption of these coumarins occurred in the course of AMVN-induced oxidative stress. Redox status, evaluated by intracellular GSH content, remained unchanged after treatment with the coumarins. tert-Butyl hydroperoxide and cumene hydroperoxide (more hydrophilic oxidants) induced a lower chemiluminescence signal with both coumarins. Viability of HL-60 cells was not affected by coumarins both in the presence and in the absence of oxidants. Based on these results we conclude that quinolizin-substituted coumarins represent a promising class of physical enhancers of chemiluminescence for monitoring free radical peroxidation in live cells.
Vladimirov
Y A
YA
Department of Environmental and Occupational Health, University of Pittsburgh, Pennsylvania 15238, USA.
Arroyo
A
A
Taylor
J M
JM
Tyurina
Y Y
YY
Matsura
T
T
Tyurin
V A
VA
Kagan
V E
VE
D001391:Azo Compounds / Q000378:metabolism; D003374:Coumarins / Q000737:chemistry / Q000378:metabolism*; D005609:Free Radicals / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D018922:HL-60 Cells; D006801:Humans; D015227:Lipid Peroxidation; D008163:Luminescent Measurements; D009570:Nitriles / Q000378:metabolism; D010084:Oxidation-Reduction; D010743:Phospholipids / Q000378:metabolism; D011807:Quinolizines / Q000737:chemistry
D001391:Azo Compounds; D003374:Coumarins; D005609:Free Radicals; D009570:Nitriles; D010743:Phospholipids; D011807:Quinolizines; C055792:2,2'-azobis(2,4-dimethylvaleronitrile); D005978:Glutathione