Identification of canine alpha-lactalbumin.
The nucleotide sequence of canine alpha-lactalbumin cDNA from canine mammary tissue was determined by polymerase chain reaction with degenerate primers. A 742 base pairs nucleotide sequence cloned was similar to the size of mRNA in Northern blot analysis. The cDNA encodes 142 amino acid residues containing the conserved sequence motif of alpha-lactalbumin, demonstrating the highest homology with pig (73% identity-82% similarity) among the known amino acid sequences of alpha-lactalbumin. The canine cDNA also showed 71% identity-78% similarity with human, 58-73% with mouse, 60-74% with rat, 67-77% with goat, 66-77% with cattle, and 67-76% with sheep, respectively.
Watanabe
M
M
Department of Veterinary Pathology, Faculty of Agriculture, Miyazaki University, Gakuen KibanaDai, Japan.
Tateyama
S
S
Togashi
T
T
Uchida
K
K
Yamaguchi
R
R
Shimizu
T
T
Sugano
S
S
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D017124:Conserved Sequence; D017931:DNA Primers; D004285:Dogs / Q000235:genetics*; D005260:Female; D006041:Goats; D006801:Humans; D007768:Lactalbumin / Q000737:chemistry / Q000235:genetics*; D008321:Mammary Glands, Animal / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D051381:Rats; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012756:Sheep; D013552:Swine
D017931:DNA Primers; D007768:Lactalbumin