Histatin 5 inhibits inflammatory cytokine induction from human gingival fibroblasts by Porphyromonas gingivalis.
Porphyromonas gingivalis is a gram-negative rod associated with the progression of human periodontal disease. It has been demonstrated that outer-membrane proteins as well as lipopolysaccharides from P. gingivalis ATCC 53977 can induce interleukin 6 (IL-6) and IL-8 from the cells of the periodontium in vitro. But, they cannot induce IL-1 and tumor necrosis factor-alpha from the cells. In the present study, we studied the effects of salivary protein on cytokine induction from human gingival fibroblasts by P. gingivalis outer-membrane protein. Histatin 5 suppressed the IL-6 and IL-8 induction by P. gingivalis outer-membrane protein. This activity was more effective when outer-membrane protein was incubated with histatin 5 before addition to the cell culture. The present study indicates that histatin 5 restrains induction of inflammatory cytokines by periodontal pathogens and that histatin is one of the salivary proteins responsible for this activity.
Imatani
T
T
Oral Health Science Center, Tokyo Dental College, Chiba, Japan.
Kato
T
T
Minaguchi
K
K
Okuda
K
K
D001425:Bacterial Outer Membrane Proteins / Q000502:physiology; D016207:Cytokines / Q000096:biosynthesis*; D005347:Fibroblasts / Q000378:metabolism / Q000382:microbiology; D005881:Gingiva / Q000166:cytology / Q000378:metabolism / Q000382:microbiology*; D054808:Histatins; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis; D016209:Interleukin-8 / Q000096:biosynthesis; D008070:Lipopolysaccharides; D016966:Porphyromonas gingivalis / Q000187:drug effects* / Q000472:pathogenicity* / Q000502:physiology; D012471:Salivary Proteins and Peptides / Q000494:pharmacology* / Q000502:physiology*
D001425:Bacterial Outer Membrane Proteins; D016207:Cytokines; C068075:HTN3 protein, human; D054808:Histatins; D015850:Interleukin-6; D016209:Interleukin-8; D008070:Lipopolysaccharides; D012471:Salivary Proteins and Peptides