Continuous marennin production by agar-entrapped Haslea ostrearia using a tubular photobioreactor with internal illumination.
The marine diatom Haslea ostrearia was immobilized in a tubular agar gel layer introduced into a photobioreactor of original design with internal illumination for the continuous synthesis of marennin, a blue-green pigment of biotechnological interest. Marennin was produced for a long-term period (27-43 days) and the volumetric productivity was maximum (18.7 mg day(-1) l(-1) gel) at the highest dilution rate (0.25 day(-1)) and lowest agar layer thickness (3 mm). Heterogeneous cell distribution in the agar layer revealed diffusional limitation of light and nutrients. However, the 3 mm gel thickness led to a more homogeneous cell distribution during incubation and to an increase of the whole biomass in the agar gel layer.
Lebeau
T
T
IUT de Colmar, Département Génie Biologique, France. thierry.lebeau@colmar.uha.fr
Gaudin
P
P
Junter
G A
GA
Mignot
L
L
Robert
J M
JM
D000362:Agar; D000818:Animals; D019149:Bioreactors; D018914:Cells, Immobilized; D003470:Culture Media; D017377:Diatoms / Q000378:metabolism*; D007700:Kinetics; D008027:Light; D009566:Nitrates / Q000378:metabolism; D010860:Pigments, Biological / Q000096:biosynthesis*
D003470:Culture Media; D009566:Nitrates; D010860:Pigments, Biological; D000362:Agar