New host-vector system for Thermus spp. based on the malate dehydrogenase gene.
A Thermus thermophilus HB27 strain was constructed in which the malate dehydrogenase (mdh) gene was deleted. The Deltamdh colonies are recognized by a small-colony phenotype. Wild-type phenotype is restored by transformation with Thermus plasmids or integration vector containing an intact mdh gene. The wild-type phenotype provides a positive selection tool for the introduction of plasmid DNA into Thermus spp., and because mdh levels can be readily quantified, this host-vector system is a convenient tool for monitoring gene expression.
Kayser
K J
KJ
Gas Technology Institute, Des Plaines, Illinois 60018, USA. kayserkj@igt.org
Kilbane
J J
JJ
D017353:Gene Deletion; D017930:Genes, Reporter; D005822:Genetic Vectors; D008291:Malate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D013824:Thermus / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D016963:Thermus thermophilus / Q000201:enzymology / Q000235:genetics / Q000254:growth & development
D008291:Malate Dehydrogenase