title: MPBLAST : improved BLAST performance with multiplexed queries.
abstract: We have developed a program, MPBLAST, that increases the throughput of batch BLASTN searches by multiplexing (concatenating) query sequences and thereby reducing the number of actual database searches performed. Throughput was observed to increase in reciprocal proportion to the component sequence length. For sequencing read-sized queries of 500 bp, an order of magnitude speed-up was seen.
  
  
  BACKGROUND
  Free (see http://blast.wustl.edu)
  
  
  BACKGROUND
  [ikorf, gish]@watson.wustl.edu
authors.affiliation: Genome Sequencing Center and Department of Genetics, Washington University School of Medicine, St Louis, MO 63108, USA. ikorf@watson.wustl.edu
authors.forename: I
authors.initials: I
authors.lastname: Korf
authors.forename: W
authors.initials: W
authors.lastname: Gish
mesh_terms: D019295:Computational Biology; D016208:Databases, Factual; D020224:Expressed Sequence Tags; D020407:Internet; D017421:Sequence Analysis / Q000706:statistics & numerical data*; D012984:Software