title: The effect of heat- and auto-polymerized denture base polymers on clonogenicity, apoptosis, and necrosis in fibroblasts: denture base polymers induce apoptosis and necrosis.
abstract: Eluates from poly(methyl methacrylate)-based denture base polymers have recently been found to enhance death by apoptosis and necrosis in U-937 human monoblastoid cells. The present study investigated the potential of such polymers to induce apoptosis and/or necrosis and to alter clonogenicity in L929 murine fibroblasts. A fibroblast cell line was chosen because the impairment of fibroblasts subjacent to denture bases may result in a weaker or more permeable mucosa. Two aspects were addressed: the effect of direct contact with the denture base polymers and the effect of eluates extracted from the polymers. For this purpose L929 fibroblasts were seeded on disks manufactured from three heat-polymerized and four autopolymerized denture base polymers or in different concentrations of their eluates. The effects were evaluated by light, fluorescent, confocal and electron microscopy, counting of colonies, and flow cytometry. Disks and eluates of all polymers enhanced cell death by apoptosis and necrosis in L929 cells and decreased their clonogenic potential in a dose-dependent manner. Apoptosis was the main form of cell death. In general, the deleterious effects were stronger when cells were plated directly on the polymer disks than in the eluates. The autopolymerized polymers, except one, yielded higher percentages of apoptosis and necrosis than the heat-polymerized polymers. The results of the study indicated that poly(methyl methacrylate)-based denture base polymers trigger death-signals in L929 fibroblasts and open doors for possible modulation of the cell/biomaterial interaction.
authors.affiliation: Department of Odontology-Dental Biomaterials, Faculty of Dentistry, University of Bergen, Norway. Mihaela-Roxana.Cimpan@cih.uib.no
authors.forename: M R
authors.initials: MR
authors.lastname: Cimpan
authors.forename: R
authors.initials: R
authors.lastname: Matre
authors.forename: L I
authors.initials: LI
authors.lastname: Cressey
authors.forename: B
authors.initials: B
authors.lastname: Tysnes
authors.forename: S A
authors.initials: SA
authors.lastname: Lie
authors.forename: B T
authors.initials: BT
authors.lastname: Gjertsen
authors.forename: N
authors.initials: N
authors.lastname: Skaug
mesh_terms: D000818:Animals; D017304:Annexin A5; D017209:Apoptosis / Q000187:drug effects*; D001672:Biocompatible Materials / Q000737:chemistry / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D003114:Colony-Forming Units Assay; D003819:Denture Bases / Q000009:adverse effects*; D016193:G1 Phase / Q000187:drug effects*; D007739:L Cells / Q000187:drug effects; D008422:Materials Testing; D051379:Mice; D009336:Necrosis; D019904:Polymethyl Methacrylate / Q000737:chemistry / Q000633:toxicity*
chemical_list: D017304:Annexin A5; D001672:Biocompatible Materials; D019904:Polymethyl Methacrylate