Nickel-resistance-based minitransposons: new tools for genetic manipulation of environmental bacteria.
The ncc and nre nickel resistance determinants from Ralstonia eutropha-like strain 31A were used to construct mini-Tn5 transposons. Broad host expression of nickel resistance was observed for the nre minitransposons in members of the alpha, beta, and gamma subclasses of the Proteobacteria, while the ncc minitransposons expressed nickel resistance only in R. eutropha-like strains.
Taghavi
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S
Vlaamse Instelling voor Technologisch Onderzoek (Vito), Environmental Technology Centre, Boeretang 200, 2400 Mol, Belgium.
Delanghe
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H
Lodewyckx
C
C
Mergeay
M
M
van der Lelie
D
D
D003227:Conjugation, Genetic; D020118:Cupriavidus necator / Q000187:drug effects* / Q000235:genetics; D004251:DNA Transposable Elements; D004352:Drug Resistance, Microbial / Q000235:genetics; D004783:Environmental Microbiology; D009532:Nickel / Q000494:pharmacology*; D020560:Proteobacteria / Q000187:drug effects* / Q000235:genetics
D004251:DNA Transposable Elements; D009532:Nickel