[AFLP markers of restoring genes of the wild-abortive hybrid rice].
Fertile and sterile pools were set up by bulked segregant analysis (BSA) based on the selection for the highly fertile and highly sterile plants of Shanyou 63 F2 population. The AFLP analysis of the two pools indicated that 64 primer combinations amplified 3,477 stable and clear bands. Exception of the combination E-AGC/M-CAA, all primer combinations had not detected polymorphism between the two pools. It was proved by investigation of two parents, individuals of F2 segregant population, backbone sterile lines and restorer lines that the polymorphic fragment AP1 generated from the primer E-AGC/M-CAA was associated with the restoring gene. AP1 was a single copy detected by Southern blot hybridization. The distance between AP1 and the restoring gene was 4.76 cM.
He
G H
GH
Center of Biotechnology, Southwest Agricultural University, Chongqing 400716, China.
Pei
Y
Y
Yang
G W
GW
Tang
M
M
Xie
R
R
Hou
L
L
Yang
Z L
ZL
Li
Y H
YH
D017343:Genes, Plant; D005819:Genetic Markers; D012275:Oryza / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length
D005819:Genetic Markers