Study of biodistribution of methotrexate-loaded bovine serum albumin nanospheres in mice.
Nanospheres made from natural hydrophilic polymers have been proved efficient in terms of better drug-loading capacity, biocompatibility, and possibility less opsonization by reticuloendothelial system (RES) through an aqueous stearic barrier. Hence, nanospheres containing methotrexate were prepared from bovine serum albumin (BSA) by a novel pH coacervation method. A drug-to-polymer ratio study was carried out to determine the carrier capacity. The batch with the highest drug loading was subjected to in vitro analysis. It was found to provide a slow release after an initial burst release. Biodistribution of nanosphere-bound drug was compared with that of free drug in mice. It was observed that the percentage increase in drug distribution to the lungs, liver, and spleen was markedly high from the nanosphere when compared to free drug.
Santhi
K
K
J. S. S. Center for Research and Post-Graduate Studies, JSS College of Pharmacy, Ooty, India.
Dhanaraj
S A
SA
Koshy
M
M
Ponnusankar
S
S
Suresh
B
B
D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D001672:Biocompatible Materials; D016503:Drug Delivery Systems; D006863:Hydrogen-Ion Concentration; D008727:Methotrexate / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D051379:Mice; D011108:Polymers; D012710:Serum Albumin, Bovine; D014018:Tissue Distribution
D000964:Antimetabolites, Antineoplastic; D001672:Biocompatible Materials; D011108:Polymers; D012710:Serum Albumin, Bovine; D008727:Methotrexate