Genomic exploration of the hemiascomycetous yeasts: 2. Data generation and processing.
The generation of sequencing data for the hemiascomycetous yeast random sequence tag project was performed using the procedures established at GENOSCOPE. These procedures include a series of protocols for the sequencing reactions, using infra-red labelled primers, performed on both ends of the plasmid inserts in the same reaction tube, and their analysis on automated DNA sequencers. They also include a package of computer programs aimed at detecting potential assignation errors, selecting good quality sequences and estimating their useful length.
Artiguenave
F
F
GENOSCOPE, Centre National de Séquençage, 2 rue Gaston Crémieux, P.O. Box 91, Cedex, F-Evry, France. artigue@genoscope.cns.fr
Wincker
P
P
Brottier
P
P
Duprat
S
S
Jovelin
F
F
Scarpelli
C
C
Verdier
J
J
Vico
V
V
Weissenbach
J
J
Saurin
W
W
D001203:Ascomycota / Q000235:genetics*; D019295:Computational Biology / Q000379:methods*; D016681:Genome, Fungal; D010843:Pichia / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods*