title: Rapid assessment of adenovirus serum neutralizing antibody titer based on quantitative, morphometric evaluation of capsid binding and intracellular trafficking: population analysis of adenovirus capsid association with cells is predictive of adenovirus infectivity.
abstract: Neutralizing antiviral antibodies are typically detected on the basis of inhibition of viral function, such as propagation of a viral infection or inhibition of viral gene expression. Evidence is presented that anti-adenovirus neutralizing antibodies can be evaluated by analysis of cell-associated capsids or by analysis of intracellular trafficking of the capsids within 1 h after infection. Quantitative analyses of these morphologic parameters represent rapid, broadly applicable, functional assays for the detection of anti-adenovirus neutralizing antibodies.
authors.affiliation: Division of Pulmonary and Critical Care Medicine, New York, New York, and the Karolinska Institute, Stockholm, Sweden.
authors.forename: T
authors.initials: T
authors.lastname: Vincent
authors.forename: B G
authors.initials: BG
authors.lastname: Harvey
authors.forename: S M
authors.initials: SM
authors.lastname: Hogan
authors.forename: C J
authors.initials: CJ
authors.lastname: Bailey
authors.forename: R G
authors.initials: RG
authors.lastname: Crystal
authors.forename: P L
authors.initials: PL
authors.lastname: Leopold
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D000914:Antibodies, Viral / Q000097:blood*; D001692:Biological Transport; D002213:Capsid / Q000378:metabolism*; D002460:Cell Line; D015316:Genetic Therapy; D006801:Humans; D008856:Microscopy, Fluorescence
chemical_list: D000914:Antibodies, Viral