Effect of introns and AT-rich sequences on expression of the bacterial hygromycin B resistance gene in the basidiomycete Schizophyllum commune.
Previously, it was shown that introns are required for efficient mRNA accumulation in Schizophyllum commune and that the presence of AT-rich sequences in the coding region of genes can result in truncation of transcripts in this homobasidiomycete. Here we show that intron-dependent mRNA accumulation and truncation of transcripts are two independent events that both affect expression of the bacterial hygromycin B resistance gene in S. commune.
Scholtmeijer
K
K
Molecular Plant Biology Laboratory, Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9751 NN Haren, The Netherlands. K.Scholtmeijer@chem.rug.nl
Wösten
H A
HA
Springer
J
J
Wessels
J G
JG
D020856:AT Rich Sequence / Q000235:genetics*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001483:Base Sequence; D004352:Drug Resistance, Microbial / Q000235:genetics; D006921:Hygromycin B / Q000494:pharmacology*; D007438:Introns / Q000235:genetics*; D008969:Molecular Sequence Data; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012567:Schizophyllum / Q000187:drug effects* / Q000235:genetics*
D000900:Anti-Bacterial Agents; D012331:RNA, Fungal; D012333:RNA, Messenger; D006921:Hygromycin B