Multiple regulatory mechanisms act on the 5' untranslated region of the S-layer gene from Thermus thermophilus HB8.
The role of the 5' untranslated region (5'UTR) of the S-layer gene from Thermus thermophilus was analyzed through the isolation of Delta 5'UTR mutants. In these mutants the half-life of splA mRNA was strongly reduced and slpA transcription was no longer subjected to growth phase-dependent repression. Overproduction and detachment of the external envelopes of the mutants were observed in stationary phase.
Castán
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P
Departamento and Centro de Biología Molecular Severo Ochoa, CSIC-UAM, CSIC Campus de Cantoblanco, Madrid 28049, Spain.
de Pedro
M A
MA
Risco
C
C
Vallés
C
C
Fernández
L A
LA
Schwarz
H
H
Berenguer
J
J
D020121:5' Untranslated Regions / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D017384:Sequence Deletion; D016963:Thermus thermophilus / Q000166:cytology / Q000235:genetics*
D020121:5' Untranslated Regions; D001426:Bacterial Proteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; C055764:S-layer proteins; C070683:surface layer protein A, Bacteria