title: Distinctive features of Drosophila alternative splicing factor RS domain: implication for specific phosphorylation, shuttling, and splicing activation.
abstract: The human splicing factor 2, also called human alternative splicing factor (hASF), is the prototype of the highly conserved SR protein family involved in constitutive and regulated splicing of metazoan mRNA precursors. Here we report that the Drosophila homologue of hASF (dASF) lacks eight repeating arginine-serine dipeptides at its carboxyl-terminal region (RS domain), previously shown to be important for both localization and splicing activity of hASF. While this difference has no effect on dASF localization, it impedes its capacity to shuttle between the nucleus and cytoplasm and abolishes its phosphorylation by SR protein kinase 1 (SRPK1). dASF also has an altered splicing activity. While being competent for the regulation of 5' alternative splice site choice and activation of specific splicing enhancers, dASF fails to complement S100-cytoplasmic splicing-deficient extracts. Moreover, targeted overexpression of dASF in transgenic flies leads to higher deleterious developmental defects than hASF overexpression, supporting the notion that the distinctive structural features at the RS domain between the two proteins are likely to be functionally relevant in vivo.
authors.affiliation: Institut de Génétique Moléculaire, UMR5535 du CNRS, IFR 24, F34293 Montpellier Cedex 5, Toulouse, France.
authors.forename: E
authors.initials: E
authors.lastname: Allemand
authors.forename: R
authors.initials: R
authors.lastname: Gattoni
authors.forename: H M
authors.initials: HM
authors.lastname: Bourbon
authors.forename: J
authors.initials: J
authors.lastname: Stevenin
authors.forename: J F
authors.initials: JF
authors.lastname: Cáceres
authors.forename: J
authors.initials: J
authors.lastname: Soret
authors.forename: J
authors.initials: J
authors.lastname: Tazi
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D001665:Binding Sites / Q000235:genetics; D002460:Cell Line; D004330:Drosophila / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D005816:Genetic Complementation Test; D006367:HeLa Cells; D006801:Humans; D019476:Insect Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D017434:Protein Structure, Tertiary; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020449:Repetitive Sequences, Amino Acid; D017412:Ribonucleoprotein, U1 Small Nuclear / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D000068103:Serine-Arginine Splicing Factors
chemical_list: D019476:Insect Proteins; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D017412:Ribonucleoprotein, U1 Small Nuclear; D000068103:Serine-Arginine Splicing Factors