title: Changes in calcium kinetics in adolescent girls induced by high calcium intake.
abstract: To identify the mechanism/s whereby calcium retention is increased by calcium intake in adolescent girls, kinetic studies were performed using stable calcium isotope tracers. Girls (n = 10; 12 +/- 1 yr old, mean +/- SD) were studied while on a controlled diet containing a low (21.2 mmol/day) and a high (47.4 mmol/day) calcium intake, in randomized order, using a cross-over design. Studies were separated by 1 month. Calcium tracers were administered after 1 week on the study diet, orally and iv; and serum, urine, and feces were collected for the following 14 days. Tracers were measured using fast atom bombardment mass spectrometry, and kinetic data were analyzed by compartmental modeling. Biochemical markers of bone turnover were measured in serum and urine samples. On high (compared with low) calcium intake, fractional absorption did not differ, absorbed calcium increased (19.6 +/- 7.5 vs. 8.0 +/- 2.5 mmol/day, mean +/- SD, P: < 0.001), calcium excreted in urine increased (2.8 +/- 1.7 vs. 2.1 +/- 1.1 mmol/day, P: < 0.01), calcium retained in bone increased (14.5 +/- 8.9 vs. 3.2 +/- 3.6 mmol/day, P: < 0.001), bone formation did not change, and bone resorption decreased by 32%. These changes, measured by kinetics, were corroborated by changes in markers of bone turnover. We conclude that increased bone retention of calcium, with high calcium intake in adolescent girls, is attributable to an increase in absorption and a decrease in bone resorption.
authors.affiliation: Division of Neonatology, Georgetown University Medical Center, Washington, DC 20007, USA. wastneym@drc.co.nz
authors.forename: M E
authors.initials: ME
authors.lastname: Wastney
authors.forename: B R
authors.initials: BR
authors.lastname: Martin
authors.forename: M
authors.initials: M
authors.lastname: Peacock
authors.forename: D
authors.initials: D
authors.lastname: Smith
authors.forename: X Y
authors.initials: XY
authors.lastname: Jiang
authors.forename: L A
authors.initials: LA
authors.lastname: Jackman
authors.forename: C M
authors.initials: CM
authors.lastname: Weaver
mesh_terms: D000293:Adolescent; D015415:Biomarkers; D001842:Bone and Bones / Q000378:metabolism; D002118:Calcium / Q000493:pharmacokinetics*; D002136:Calcium, Dietary / Q000494:pharmacology*; D002648:Child; D018592:Cross-Over Studies; D004032:Diet; D005243:Feces / Q000737:chemistry; D005260:Female; D006801:Humans; D008954:Models, Biological
chemical_list: D015415:Biomarkers; D002136:Calcium, Dietary; D002118:Calcium