Treatment of carbon tetrachloride and phenobarbital-induced chronic liver failure with intrasplenic hepatocyte transplantation.
Hepatocyte transplantation (HTx) has been shown to improve the survival of laboratory animals with experimentally induced acute liver failure and to ameliorate the physiologic abnormalities associated with liver-based metabolic deficiencies. However, the role of HTx in the treatment of liver cirrhosis (LC) has not been adequately studied. In order to address this issue, HTx was performed in rats following induction of stable LC using phenobarbital (PhB) and carbon tetrachloride (CCl4). Intrasplenic transplantation of 50 x 10(6) primary hepatocytes could significantly improve liver functions and prolong the survival of rats with irreversible, decompensated LC.
Kobayashi
N
N
Department of Surgery, University of Nebraska Medical Center, Omaha 68198, USA.
Ito
M
M
Nakamura
J
J
Cai
J
J
Hammel
J M
JM
Fox
I J
IJ
D000818:Animals; D002251:Carbon Tetrachloride / Q000008:administration & dosage; D002908:Chronic Disease; D022781:Hepatocytes / Q000637:transplantation*; D008106:Liver Cirrhosis, Experimental / Q000473:pathology / Q000601:surgery*; D017093:Liver Failure / Q000139:chemically induced / Q000473:pathology / Q000601:surgery*; D008297:Male; D010634:Phenobarbital / Q000008:administration & dosage; D011184:Postoperative Period; D000091402:Prohibitins; D051381:Rats; D011917:Rats, Inbred Lew; D013154:Spleen / Q000601:surgery; D015996:Survival Rate; D016042:Transplantation, Heterotopic
C000717332:Phb protein, rat; D000091402:Prohibitins; D002251:Carbon Tetrachloride; D010634:Phenobarbital