COX and LOX eicosanoids modulate platelet activation and procoagulation induced by two murine cancer cells.
Involvement of arachidonic acid cyclooxygenase (COX) and lipoxygenase (LOX) metabolites in platelet aggregation and coagulation induced by two varieties of cancer cells of murine transplantable tumors was studied. A lung alveolar carcinoma (LAC) and a fibrosarcoma (FS), induced platelet aggregation and plasma coagulation (P<0.05). Pretreatment of both tumor lines with a COX inhibitor did not block the tumor cell induced platelet aggregation (TCIPA). COX [12(S)-HTT] and LOX [12(S)-HETE], metabolites of washed platelets (WP), alone or co-incubated with LAC or FS cells, were analyzed. We observed higher 12(S)-HETE release with respect to 12(S)HHT when WP were co-incubated with LAC cells. With both neoplastic cell (NC) lines prothrombin time (PT) was shortened. Pretreatment of NC with iodoacetic acid, soybean trypsin inhibitor or Factor X-deficient plasma increased the PT. These results indicate that AA metabolites play a role on the procoagulation and platelet aggregation induced by mesenchymal and epithelial murine cancers.
Pasqualini
M E
ME
Departamento de Histología, Embriología y Genética, Instituto de Biología Celular, Facultad de Ciencias, Universidad Nacional de Córdoba, Argentina.
Mohn
C E
CE
Petiti
J P
JP
Manzo
P
P
Eynard
A R
AR
D002282:Adenocarcinoma, Bronchiolo-Alveolar / Q000378:metabolism / Q000473:pathology*; D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism; D001777:Blood Coagulation / Q000187:drug effects*; D018920:Coculture Techniques; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000378:metabolism*; D015777:Eicosanoids / Q000096:biosynthesis / Q000145:classification / Q000502:physiology*; D005170:Factor X / Q000502:physiology; D005354:Fibrosarcoma / Q000378:metabolism / Q000473:pathology*; D019807:Iodoacetic Acid / Q000494:pharmacology; D008084:Lipoxygenase / Q000378:metabolism*; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009363:Neoplasm Proteins; D015539:Platelet Activation / Q000187:drug effects*; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D019851:Thrombophilia / Q000209:etiology / Q000503:physiopathology; D014360:Trypsin Inhibitor, Kunitz Soybean / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
D001095:Arachidonic Acids; D015777:Eicosanoids; D009363:Neoplasm Proteins; D005170:Factor X; D014360:Trypsin Inhibitor, Kunitz Soybean; D008084:Lipoxygenase; D011451:Prostaglandin-Endoperoxide Synthases; D003546:Cysteine Endopeptidases; C030910:cancer procoagulant; D019807:Iodoacetic Acid