Optimized indirect (76)Br-bromination of antibodies using N-succinimidyl para-[76Br]bromobenzoate for radioimmuno PET.
Monoclonal antibody 38S1 was radiobrominated with the positron emitter (76)Br (T(1/2) = 16.2 h). Indirect labeling was performed using N-succinimidyl para-(tri-methylstannyl)benzoate (SPMB) as the precursor molecule. SPMB was labeled using Chloramine-T yielding N-succinimidyl para-[(76)Br]bromobenzoate, which was then conjugated to the antibody. Optimization of the labeling conditions and further conjugation gave a total yield ( mean+/-max error) of 49+/-2%. The immunoreactivity of the antibodies was retained after labeling. Thus, antibodies intended for positron emission tomography can be labeled with (76)Br, which gives high yields and preserved immunoreactivity when using the SPMB technique described.
Höglund
J
J
Department of Oncology, Radiology, and Clinical Immunology, Uppsala University, Uppsala, Sweden. Johanna.Sundin@pet.uu.se
Tolmachev
V
V
Orlova
A
A
Lundqvist
H
H
Sundin
A
A
D000911:Antibodies, Monoclonal / Q000737:chemistry*; D001565:Benzoates / Q000737:chemistry*; D001967:Bromine Radioisotopes; D002272:Carcinoembryonic Antigen / Q000737:chemistry / Q000276:immunology; D007202:Indicators and Reagents; D007553:Isotope Labeling; D019275:Radiopharmaceuticals / Q000138:chemical synthesis*; D013388:Succinimides / Q000737:chemistry*; D014055:Tomography, Emission-Computed
D000911:Antibodies, Monoclonal; D001565:Benzoates; D001967:Bromine Radioisotopes; D002272:Carcinoembryonic Antigen; D007202:Indicators and Reagents; C422194:N-succinimidyl 4-bromobenzoate; D019275:Radiopharmaceuticals; D013388:Succinimides