cDNA micro array identification of a gene differentially expressed in adenovirus type 5- versus type 12-transformed cells.
Proteins encoded by non-oncogenic adenovirus type 5 and oncogenic adenovirus type 12 differentially affect expression of a number of cellular genes. We have used cDNA micro array analysis to identify a cellular gene that is expressed in Ad12- but not in Ad5-transformed cells. This cellular gene was found to be the gene encoding follistatin-related protein, a TGF-beta inducible gene. Consistently, a constitutive factor binding to Smad binding elements was found in adenovirus type 12-transformed cells.
Vertegaal
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Laboratory for Molecular Carcinogenesis, MGC-Department of Molecular Cell Biology, Leiden University Medical Center, Wassenaarseweg 72, 2333 AL, Leiden, The Netherlands.
Kuiperij
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HB
van Laar
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Scharnhorst
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van der Eb
A J
AJ
Zantema
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A
D000260:Adenoviruses, Human / Q000235:genetics*; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D020224:Expressed Sequence Tags; D038702:Follistatin-Related Proteins; D005786:Gene Expression Regulation / Q000187:drug effects; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D007668:Kidney; D008168:Lung; D020411:Oligonucleotide Array Sequence Analysis; D012160:Retina; D013045:Species Specificity; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D038702:Follistatin-Related Proteins; D006023:Glycoproteins; D016212:Transforming Growth Factor beta