Application of rapid dot blot immunoassay for detection of Salmonella enterica serovar enteritidis in eggs, poultry, and other foods.
Salmonella enterica serovar Enteritidis was detected in artificially inoculated eggs within 24 h through a rapid monoclonal antibody-based dot blot immunoassay. Detection in poultry and other products required 28 h. Samples were directly enriched in homogenized egg without the need for pre- or postenrichment steps. Serovar Enteritidis was detected in the presence of other bacteria when outcompeted 1:400.
Yoshimasu
M A
MA
Department of Food Science, University of Manitoba, Winnipeg, Manitoba, Canada R3T 2N2.
Zawistowski
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D003470:Culture Media; D004531:Eggs / Q000382:microbiology; D005516:Food Microbiology; D015151:Immunoblotting / Q000379:methods*; D011200:Poultry / Q000382:microbiology; D012477:Salmonella enteritidis / Q000276:immunology / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D003470:Culture Media