title: Metabolites of oxytetracycline, tetracycline, and chlortetracycline and their distribution in egg white, egg yolk, and hen plasma.
abstract: 4-epioxytetracycline and N-demethyloxytetracycline, as metabolites of oxytetracycline (OTC), 4-epitetracycline and N-demethyltetracycline, as metabolites of tetracycline (TC), and 4-epichlortetracycline, isochlortetracycline (ICTC), 4-epi-ICTC, and N-demethyl-ICTC, as metabolites of chlortetracycline (CTC), were detected in egg yolk and plasma obtained from feeding studies with either OTC, TC, or CTC. In egg white, only OTC, TC with its 4-epimer, and ICTC with its 4-epimer were detected in substantial concentrations. The ratios of epimerization and N-demethylation in the eggs did not change during the medication period. The samples were analyzed by an automated HPLC system (ASTED) with UV, fluorescence, or MS-MS detection.
authors.affiliation: Department of Food Chemistry, University of Wuppertal, Gauss-Strasse 20, D-42097 Wuppertal, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Zurhelle
authors.forename: M
authors.initials: M
authors.lastname: Petz
authors.forename: E
authors.initials: E
authors.lastname: Mueller-Seitz
authors.forename: E
authors.initials: E
authors.lastname: Siewert
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000378:metabolism; D002645:Chickens / Q000097:blood; D002751:Chlortetracycline / Q000032:analysis / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004529:Egg White / Q000032:analysis; D004530:Egg Yolk / Q000737:chemistry; D004531:Eggs / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D010118:Oxytetracycline / Q000032:analysis / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013752:Tetracycline / Q000032:analysis / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D000900:Anti-Bacterial Agents; D013752:Tetracycline; D002751:Chlortetracycline; D010118:Oxytetracycline