Platelet-derived growth factor is involved in the augmentation of airway responsiveness through remodeling of airways in diesel exhaust particulate-treated mice.
BACKGROUND
Thickening of the region adjacent to the basement membrane is a key component of the remodeling of the asthmatic airway and is caused by collagen deposition in the region.


OBJECTIVE
We sought to clarify the role of platelet-derived growth factor (PDGF), a competence factor of fibroblast, in the enhanced airway responsiveness and remodeling in a murine model.


METHODS
Diesel exhaust particulates (DEPs) were administered intranasally every other day for 2 weeks with or without anti-PDGF-beta neutralizing antibody or goat IgG. Pulmonary function was then analyzed by using whole-body plethysmography before and after acetylcholine inhalation.


RESULTS
Anti-PDGF-beta neutralizing antibody significantly inhibited both the elevation of airway resistance elicited by 1.25 and 2.5 mg/mL acetylcholine and the increase in the airway wall thickening induced by DEPs. In addition, bronchoalveolar lavage fluid cell analysis revealed that anti-PDGF-beta neutralizing antibody did not affect cellular infiltration at the airways.


CONCLUSIONS
PDGF plays an important role in the process of remodeling brought about by DEP exposure in mice.
Yamashita
N
N
Department of Medicine, Teikyo University School of Medicine, Tokyo, Japan.
Sekine
K
K
Miyasaka
T
T
Kawashima
R
R
Nakajima
Y
Y
Nakano
J
J
Yamamoto
T
T
Horiuchi
T
T
Hirai
K
K
Ohta
K
K
D016475:3T3 Cells / Q000187:drug effects; D000403:Airway Resistance / Q000187:drug effects*; D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D001249:Asthma / Q000503:physiopathology; D016535:Bronchial Hyperreactivity / Q000188:drug therapy* / Q000209:etiology / Q000503:physiopathology*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D002455:Cell Division / Q000276:immunology; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D010982:Platelet-Derived Growth Factor / Q000276:immunology / Q000494:pharmacology*; D012137:Respiratory System / Q000737:chemistry / Q000473:pathology; D013194:Staining and Labeling; D001335:Vehicle Emissions / Q000009:adverse effects*
D000906:Antibodies; D010982:Platelet-Derived Growth Factor; D001335:Vehicle Emissions