title: Plasmid-mediated and inducible cephalosporinase DHA-2 from Klebsiella pneumoniae.
abstract: A Klebsiella pneumoniae strain resistant to cefoxitin and oxyimino-cephalosporins was cultured from a child hospitalized in Paris, France, in 1992. This isolate harboured a beta-lactamase gene located on an approximately 200 kb non-self-transferable plasmid. The beta-lactamase identified, DHA-2, shared 99% amino acid identity with the AmpC enzyme of Morganella morganii. DHA-2 was a point-mutant derivative of DHA-1 identified previously in a Salmonella enteritidis isolate. DHA-2 expression was inducible due to an ampR regulatory gene. This is the first report of an inducible and plasmid-located cephalosporinase from K. pneumoniae.
authors.affiliation: Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 94275 Le Kremlin-Bicêtre, France.
authors.forename: N
authors.initials: N
authors.lastname: Fortineau
authors.forename: L
authors.initials: L
authors.lastname: Poirel
authors.forename: P
authors.initials: P
authors.lastname: Nordmann
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000032:analysis / Q000378:metabolism; D002442:Ceftazidime / Q000494:pharmacology; D002511:Cephalosporins / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D004790:Enzyme Induction / Q000187:drug effects; D007525:Isoelectric Focusing; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010641:Phenotype; D010957:Plasmids / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D001618:beta-Lactamases / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D002511:Cephalosporins; D002442:Ceftazidime; C427406:cephalosporinase DHA-2; D001618:beta-Lactamases