Gene targeting in livestock: a preview.
Until recently genetically modified livestock could only be generated by pronuclear injection. The discovery that animals can be cloned by nuclear transfer from cultured somatic cells means that it will now be possible to achieve gene targeting in these species. We discuss current developments in NT, the prospects and technical challenges for introducing targeted changes into the germline by this route, and the types of application for which this new technology will be used.
Clark
A J
AJ
Roslin Institute, Midlothian, UK. john.clark@bbsrc.ac.uk
Burl
S
S
Denning
C
C
Dickinson
P
P
D000818:Animals; D000829:Animals, Domestic / Q000235:genetics*; D019976:Cloning, Organism / Q000379:methods / Q000639:trends / Q000662:veterinary; D018390:Gene Targeting / Q000379:methods* / Q000639:trends / Q000662:veterinary; D053652:Nuclear Transfer Techniques