[Construction of the physical map of Pi-2(t), a blast resistance gene in rice].
In an effort to clone a rice blast resistance gene, Pi-2(t), a BAC contig consisting of 22 BAC clones covering the whole Pi-2(t) region, was constructed using marker-based chromosome landing and chromosome walking. The genetic span of this physical map is 8 cM, but the physical size is 925 kb. The physical map forms the base of further isolation and cloning of Pi-2(t) gene, it will provide new markers for molecular marker-aided selection of new blast resistance materials in rice.
Fu
B Y
BY
Institute of Genetics, College of Life Sciences, Wuhan University, Wuhan 430072, China.
Yang
D C
DC
Zhu
Y G
YG
Li
Z K
ZK
D002874:Chromosome Mapping; D016386:Chromosome Walking; D022202:Chromosomes, Artificial, Bacterial; D017343:Genes, Plant; D012275:Oryza / Q000235:genetics*; D010935:Plant Diseases / Q000235:genetics*