Structure of a serpin-enzyme complex probed by cysteine substitutions and fluorescence spectroscopy.
The x-ray crystal structure of the serpin-proteinase complex is yet to be determined. In this study we have investigated the conformational changes that take place within antitrypsin during complex formation with catalytically inactive (thrombin(S195A)) and active thrombin. Three variants of antitrypsin Pittsburgh (an effective thrombin inhibitor), each containing a unique cysteine residue (Cys(232), Cys(P3'), and Cys(313)) were covalently modified with the fluorescence probe N,N'-dimethyl-N-(iodoacetyl)-N'-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)ethylenediamine. The presence of the fluorescent label did not affect the structure or inhibitory activity of the serpin. We monitored the changes in the fluorescence emission spectra of each labeled serpin in the native and cleaved state, and in complex with active and inactive thrombin. These data show that the serpin undergoes conformational change upon forming a complex with either active or inactive proteinase. Steady-state fluorescence quenching measurements using potassium iodide were used to further probe the nature and extent of this conformational change. A pronounced conformational change is observed upon locking with an active proteinase; however, our data reveal that docking with the inactive proteinase thrombin(S195A) is also able to induce a conformational change in the serpin.
Ludeman
J P
JP
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia.
Whisstock
J C
JC
Hopkins
P C
PC
Le Bonniec
B F
BF
Bottomley
S P
SP
D019943:Amino Acid Substitution; D055592:Biophysical Phenomena; D001703:Biophysics; D018360:Crystallography, X-Ray; D003545:Cysteine / Q000737:chemistry; D005456:Fluorescent Dyes; D006801:Humans; D066298:In Vitro Techniques; D046911:Macromolecular Substances; D008958:Models, Molecular; D010069:Oxadiazoles; D011487:Protein Conformation; D012697:Serine Endopeptidases / Q000737:chemistry*; D015843:Serpins / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013917:Thrombin / Q000737:chemistry; D000515:alpha 1-Antitrypsin / Q000737:chemistry
D005456:Fluorescent Dyes; D046911:Macromolecular Substances; D010069:Oxadiazoles; D015843:Serpins; D000515:alpha 1-Antitrypsin; C038915:alpha 1-antitrypsin Pittsburgh; C035573:4-(N-(iodoacetoxy)ethyl-N-methyl)amino-7-nitrobenz-2-oxa-1,3-diazole; D012697:Serine Endopeptidases; D013917:Thrombin; D003545:Cysteine