title: Continuous marennin production by agar-entrapped Haslea ostrearia using a tubular photobioreactor with internal illumination.
abstract: The marine diatom Haslea ostrearia was immobilized in a tubular agar gel layer introduced into a photobioreactor of original design with internal illumination for the continuous synthesis of marennin, a blue-green pigment of biotechnological interest. Marennin was produced for a long-term period (27-43 days) and the volumetric productivity was maximum (18.7 mg day(-1) l(-1) gel) at the highest dilution rate (0.25 day(-1)) and lowest agar layer thickness (3 mm). Heterogeneous cell distribution in the agar layer revealed diffusional limitation of light and nutrients. However, the 3 mm gel thickness led to a more homogeneous cell distribution during incubation and to an increase of the whole biomass in the agar gel layer.
authors.affiliation: IUT de Colmar, Département Génie Biologique, France. thierry.lebeau@colmar.uha.fr
authors.forename: T
authors.initials: T
authors.lastname: Lebeau
authors.forename: P
authors.initials: P
authors.lastname: Gaudin
authors.forename: G A
authors.initials: GA
authors.lastname: Junter
authors.forename: L
authors.initials: L
authors.lastname: Mignot
authors.forename: J M
authors.initials: JM
authors.lastname: Robert
mesh_terms: D000362:Agar; D000818:Animals; D019149:Bioreactors; D018914:Cells, Immobilized; D003470:Culture Media; D017377:Diatoms / Q000378:metabolism*; D007700:Kinetics; D008027:Light; D009566:Nitrates / Q000378:metabolism; D010860:Pigments, Biological / Q000096:biosynthesis*
chemical_list: D003470:Culture Media; D009566:Nitrates; D010860:Pigments, Biological; D000362:Agar