title: The release of alginate lyase from growing Pseudomonas syringae pathovar phaseolicola.
abstract: Pseudomonas syringae pathovar phaseolicola, which produces alginate during stationary growth phase, displayed elevated extracellular alginate lyase activity during both mid-exponential and late-stationary growth phases of batch growth. Intracellular activity remained below 22% of the total activity during exponential growth, suggesting that alginate lyase has an extracellular function for this organism. Extracellular enzyme activity in continuous cultures, grown in either nutrient broth or glucose-simple salts medium, peaked at 60% of the washout rate, although nutrient broth-grown cultures displayed more than twice the activity per gram of cell mass. These results imply that growth rate, nutritional composition, or both initiate a release of alginate lyase from viable P. syringae pv. phaseolicola, which could modify its entrapping biofilm.
authors.affiliation: Audubon Sugar Institute, Louisiana Experimental Station, South Stadium Road, Baton Rouge, Louisiana 70803, USA. charlie.m.ott1@jsc.nasa.gov
authors.forename: C M
authors.initials: CM
authors.lastname: Ott
authors.forename: D F
authors.initials: DF
authors.lastname: Day
authors.forename: D W
authors.initials: DW
authors.lastname: Koenig
authors.forename: D L
authors.initials: DL
authors.lastname: Pierson
mesh_terms: D010935:Plant Diseases / Q000382:microbiology; D011133:Polysaccharide-Lyases / Q000378:metabolism*; D011549:Pseudomonas / Q000201:enzymology*
chemical_list: D011133:Polysaccharide-Lyases; C016158:poly(beta-D-mannuronate) lyase