Plasma beta-N-acetylhexosaminidase isoenzyme composition and temperature conversion factors.
Using the chromogenic substrate 3,3'-dichlorophenylsulfonphthaleinyl-N-acetyl-beta-D-glucosaminide for the activity determination of plasma beta-N-acetylhexosaminidase (Hex), the temperature conversion factors (TCF) offer a highly significant positive correlation with the relative proportion of Hex B isoenzyme (P< 0.001). The calculation of TCF 37 degrees/25 degrees C allows the isoenzyme composition of Hex to be determined quickly and cheaply. The results may be superimposed over those obtained in a previously described method based on the calculation of the enzyme's activation energy using four temperatures. However, the use of TCF 37 degrees/30 degrees C does not appear to comply with the required demands.
Casal
J A
JA
Laboratorio Central, Hospital Clínico Universitario, Santiago de Compostela, Spain.
Pérez
L F
LF
Tutor
J C
JC
D001322:Autoanalysis; D002863:Chromogenic Compounds / Q000378:metabolism; D054819:Hexosaminidase B; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D012044:Regression Analysis; D013696:Temperature; D001619:beta-N-Acetylhexosaminidases / Q000097:blood*
D002863:Chromogenic Compounds; D007527:Isoenzymes; D054819:Hexosaminidase B; D001619:beta-N-Acetylhexosaminidases