title: CD8+ T cells recognize an inclusion membrane-associated protein from the vacuolar pathogen Chlamydia trachomatis.
abstract: During infection with Chlamydia trachomatis, CD8(+) T cells are primed, even though the bacteria remain confined to a host cell vacuole throughout their developmental cycle. Because CD8(+) T cells recognize antigens processed from cytosolic proteins, the Chlamydia antigens recognized by these CD8(+) T cells very likely have access to the host cell cytoplasm during infection. The identity of these C. trachomatis proteins has remained elusive, even though their localization suggests they may play important roles in the biology of the organism. Here we use a retroviral expression system to identify Cap1, a 31-kDa protein from C. trachomatis recognized by protective CD8(+) T cells. Cap1 contains no strong homology to any known protein. Immunofluorescence microscopy by using Cap1-specific antibody demonstrates that this protein is localized to the vacuolar membrane. Cap1 is virtually identical among the human C. trachomatis serovars, suggesting that a vaccine incorporating Cap1 might enable the vaccine to protect against all C. trachomatis serovars. The identification of proteins such as Cap1 that associate with the inclusion membrane will be required to fully understand the interaction of C. trachomatis with its host cell.
authors.affiliation: Corixa Corporation, Seattle, WA 98104, USA. sfling@corixa.com
authors.forename: S P
authors.initials: SP
authors.lastname: Fling
authors.forename: R A
authors.initials: RA
authors.lastname: Sutherland
authors.forename: L N
authors.initials: LN
authors.lastname: Steele
authors.forename: B
authors.initials: B
authors.lastname: Hess
authors.forename: S E
authors.initials: SE
authors.lastname: D'Orazio
authors.forename: J
authors.initials: J
authors.lastname: Maisonneuve
authors.forename: M F
authors.initials: MF
authors.lastname: Lampe
authors.forename: P
authors.initials: P
authors.lastname: Probst
authors.forename: M N
authors.initials: MN
authors.lastname: Starnbach
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D002478:Cells, Cultured; D002692:Chlamydia trachomatis / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D005260:Female; D015723:Gene Library; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D014617:Vacuoles / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; C423325:Cap1 protein, Chlamydia trachomatis; D008565:Membrane Proteins; D009838:Oligodeoxyribonucleotides