Molecular structure, dynamic expression, and promoter analysis of zebrafish (Danio rerio) myf-5 gene.
We isolated a 1,438 bp cDNA fragment that encoded Myf-5 myogenic factor of zebrafish. The deduced amino acid contained 237 residues, including the basic helix-loop-helix domain that is conserved in all known Myf-5. The zebrafish myf-5 transcripts were first detectable at 7.5 hpf, increased substantially until 16 hpf, and then declined gradually to an undetectable level by 26 hpf. During somitogenesis, zebrafish myf-5 transcripts were distributed mainly in the somites and segmental plates. Prominent signals occurred transiently in adaxial cells in two parallel rows but did not extend beyond the positive-signal somites. Various lengths of upstream region of zebrafish myf-5 fused with EGFP gene were used to carry out transgenic analysis. Results showed that a small, 82 bp (nucleotide positions from -82 to -1), regulatory cassette is sufficient to control the somite- and stage-specific expression of zebrafish myf-5 during early development.
Chen
Y H
YH
Institute of Fisheries Science, National Taiwan University, Taipei, Taiwan, Republic of China.
Lee
W C
WC
Liu
C F
CF
Tsai
H J
HJ
D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D004268:DNA-Binding Proteins; D005091:Exons; D015870:Gene Expression; D005809:Genes, Regulator; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D017403:In Situ Hybridization; D007438:Introns; D008164:Luminescent Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000235:genetics* / Q000378:metabolism; D051787:Myogenic Regulatory Factor 5; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid; D015534:Trans-Activators; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic; D015027:Zebrafish / Q000235:genetics*
D017931:DNA Primers; D004268:DNA-Binding Proteins; D008164:Luminescent Proteins; D009124:Muscle Proteins; D051787:Myogenic Regulatory Factor 5; D012333:RNA, Messenger; D015534:Trans-Activators; D014157:Transcription Factors; D049452:Green Fluorescent Proteins