Comparative study of superoxide dismutase in normal and hereditary cataract (UPL) rats.
In the present study, the levels of SOD activity and Cu, Zn-SOD mRNA in the brain, kidney, liver and eye of normal and Upjohn Pharmaceutics Limited (UPL) rats, a new hereditary cataract model derived from Sprague-Dawley rats, were measured. Although the levels of SOD activity in the eye and brain of UPL rats were significantly decreased compared with those of normal rats 3 and 5 weeks after birth, the levels of SOD activities in the kidney and liver were the same in both groups. The levels of Cu, Zn-SOD mRNA in kidney and liver of UPL rats were the same as those of normal controls. The level of Cu, Zn-SOD mRNA in the brain of normal rats 5 weeks after birth was about twofold greater than that of UPL, and that in the eye of UPL rats 3 weeks after birth was significantly decreased compared with that of normal controls. The sequences of cDNA encoding Cu, Zn-SOD and the sequences of the regulatory region of the Cu, Zn-SOD gene were confirmed to be the same in normal and UPL rats. These results indicated that the decreases in levels of SOD activity and Cu, Zn-SOD mRNA in the brain and eye of UPL rat were not due to mutation of the genomic Cu, Zn-SOD gene in UPL rats or differences in the sequence of the regulatory region of the Cu, Zn-SOD gene between normal and UPL rats.
Komada
F
F
Department of Drug Informatics, Faculty of Pharmaceutical Sciences, Josai University, Sakado, Saitama, Japan. komada@josai.ac.jp
Saitoh
Y
Y
Kawabata
H
H
Ohsaka
N
N
Okumura
K
K
Tomohiro
M
M
Ito
Y
Y
Terao
M
M
Hori
R
R
D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000201:enzymology; D002386:Cataract / Q000201:enzymology* / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005123:Eye / Q000201:enzymology; D005260:Female; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008297:Male; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011922:Rats, Mutant Strains; D017207:Rats, Sprague-Dawley; D012045:Regulatory Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*
D018076:DNA, Complementary; D012333:RNA, Messenger; D013482:Superoxide Dismutase