title: Small-angle neutron scattering study of the n-decane effect on the bilayer thickness in extruded unilamellar dioleoylphosphatidylcholine liposomes.
abstract: Dioleoylphosphatidylcholine (DOPC) and n-decane were mixed and hydrated afterwards in an excess of heavy water at 1 wt.% of DOPC. From this dispersion, unilamellar liposomes were prepared by extrusion through polycarbonate filter with 500-A pores. Small-angle neutron scattering (SANS) was conducted on these liposomes. From the Kratky-Porod plot ln[I(Q)Q2] vs. Q2 of SANS intensity I(Q) in the range of scattering vectors Q corresponding to the interval 0.001 A(-2) < or = Q2 < or = 0.006 A(-2), the liposome bilayer radius of gyration Rg and the bilayer thickness parameter d(g) = 12(0.5)Rg were obtained. The values of d(g) indicated that the bilayer thickness is within the experimental error constant up to n-decane/DOPC approximately 0.5 molar ratio, and then increases by 2.4 +/- 1.3 A up to n-decane/DOPC = 1.2 molar ratio.
authors.affiliation: Faculty of Pharmacy, J.A. Comenius University, Bratislava, Slovakia.
authors.forename: D
authors.initials: D
authors.lastname: Uhríková
authors.forename: P
authors.initials: P
authors.lastname: Balgavý
authors.forename: N
authors.initials: N
authors.lastname: Kucerka
authors.forename: A
authors.initials: A
authors.lastname: Islamov
authors.forename: V
authors.initials: V
authors.lastname: Gordeliy
authors.forename: A
authors.initials: A
authors.lastname: Kuklin
mesh_terms: D000473:Alkanes / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D009502:Neutrons; D010713:Phosphatidylcholines / Q000737:chemistry*; D013053:Spectrophotometry / Q000379:methods
chemical_list: D000473:Alkanes; D008051:Lipid Bilayers; D008081:Liposomes; D010713:Phosphatidylcholines; C017251:1,2-oleoylphosphatidylcholine; C012867:decane