[Biochemical adaptation of the barley root cells to toxic substances. II. Effect of heavy metals on phosphohydrolase activity].
We studied the effect of heavy metals such as Cu, Zn, Cd, and Pb on the activity of membrane-bound and soluble phosphohydrolases of barley root cell apoplast. The effect of heavy metals on the activity of acid phosphatase and acid nucleotidases proved to depend on the presence of calcium and magnesium ions in the incubation buffer, the condition and form of the enzymes, as well as the dose and moment of heavy metals addition. We propose changing the activity of acid phosphohydrolases in barley root cell apoplast as a possible mechanism of barley adaptation to the toxic effect of heavy metals.
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Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya 35, Moscow, 127276 Russia.
Fedorovskaia
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D000222:Adaptation, Physiological; D000469:Alkaline Phosphatase / Q000378:metabolism; D002104:Cadmium / Q000633:toxicity; D002122:Calcium Chloride / Q000494:pharmacology; D003300:Copper / Q000633:toxicity; D001467:Hordeum / Q000187:drug effects* / Q000201:enzymology; D006868:Hydrolysis; D007854:Lead / Q000633:toxicity; D015636:Magnesium Chloride / Q000494:pharmacology; D019216:Metals, Heavy / Q000633:toxicity*; D008861:Microsomes / Q000187:drug effects / Q000201:enzymology; D009708:Nucleotidases / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D018517:Plant Roots / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D015032:Zinc / Q000633:toxicity
D019216:Metals, Heavy; D002104:Cadmium; D015636:Magnesium Chloride; D007854:Lead; D003300:Copper; D009708:Nucleotidases; D000469:Alkaline Phosphatase; D010744:Phosphoric Monoester Hydrolases; D015032:Zinc; D002122:Calcium Chloride