Magnetic bead capture eliminates PCR inhibitors in samples collected from the airborne environment, permitting detection of Pneumocystis carinii DNA.
PCR detection methods are useful in studies of organisms not amenable to culture. Inhibitors in environmental samples can interfere with such assays. We describe a magnetic bead DNA capture protocol that removes inhibitors from outdoor air samples, maintaining the sensitivity of a 16S Pneumocystis carinii mitochondrial rRNA gene-based PCR.
Maher
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Division of Geographic Medicine and Department of Environmental Health Sciences, University of Alabama at Birmingham, 35294-2170, USA.
Dillon
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Vermund
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Unnasch
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TR
D000391:Air Microbiology; D004271:DNA, Fungal / Q000032:analysis*; D004784:Environmental Monitoring / Q000379:methods*; D005374:Filtration; D008280:Magnetics; D011010:Pneumocystis / Q000235:genetics / Q000302:isolation & purification*; D011076:Polycarboxylate Cement; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D004271:DNA, Fungal; D011076:Polycarboxylate Cement; C005506:polycarbonate