title: Phospholipid identification and quantification of membrane vesicle subfractions by 31P-1H two-dimensional nuclear magnetic resonance.
abstract: An approach to the direct quantification of phospholipids from two-dimensional 31P-1H nuclear magnetic resonance (NMR) spectroscopy with isotropic proton mixing has been developed as a general method for phospholipid analysis of minor membrane vesicle subfractions. Membrane vesicles were subfractionated by sedimentation to density equilibrium in a sucrose gradient, and a modified Folch method was employed to extract their phospholipids. The coefficient for the NMR detection efficiency of each phospholipid and the relative mole percentage of the phospholipids present in the membrane vesicles were calculated. We demonstrate low detection limits such that relative concentrations of phospholipids in membrane subfractions may be determined even in the submicromolar range.
authors.affiliation: Department of Polymer Science and Engineering, University of Massachusetts at Amherst, Massachusetts 01003, USA.
authors.forename: B
authors.initials: B
authors.lastname: Larijani
authors.forename: D L
authors.initials: DL
authors.lastname: Poccia
authors.forename: L C
authors.initials: LC
authors.lastname: Dickinson
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular; D010743:Phospholipids / Q000737:chemistry*; D010759:Phosphorus Isotopes; D011522:Protons; D012617:Sea Urchins
chemical_list: D010743:Phospholipids; D010759:Phosphorus Isotopes; D011522:Protons