Salmonella vaccines secreting measles virus epitopes induce protective immune responses against measles virus encephalitis.
In the present study we describe a live vaccine against measles virus (MV) infection on the basis of attenuated Salmonella typhimurium aroA secreting MV antigens via the Escherichia coli alpha-hemolysin secretion system. Two well-characterized MV epitopes, a B-cell epitope of the MV fusion protein (amino acids 404-414) and a T-cell epitope of the MV nucleocapsid protein (amino acids 79-99) were fused as single or repeating units to the C-terminal secretion signal of the E. coli hemolysin and expressed in secreted form by the attenuated S. typhimurium aroA SL7207. Immunization of MV-susceptible C3H mice revealed that S. typhimurium SL7207 secreting these antigens provoked a humoral and a cellular MV-specific immune response, respectively. Mice vaccinated orally with a combination of both recombinant S. typhimurium strains showed partial protection against a lethal MV encephalitis after intracerebral challenge with a rodent-adapted, neurotropic MV strain.
Spreng
S
S
Lehrstuhl für Mikrobiologie, Theodor-Boveri-Institut für Biowissenschaften, Am Hubland, D-97074, Würzburg, Germany. simone@biozentrum.uni-wuerzburg.de
Gentschev
I
I
Goebel
W
W
Weidinger
G
G
ter Meulen
V
V
Niewiesk
S
S
D000284:Administration, Oral; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018985:Epitopes, B-Lymphocyte / Q000096:biosynthesis*; D018984:Epitopes, T-Lymphocyte / Q000096:biosynthesis*; D005822:Genetic Vectors; D008459:Measles virus / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D010957:Plasmids; D011994:Recombinant Proteins / Q000276:immunology; D012475:Salmonella; D013344:Subacute Sclerosing Panencephalitis / Q000517:prevention & control*; D014765:Viral Vaccines
D018985:Epitopes, B-Lymphocyte; D018984:Epitopes, T-Lymphocyte; D011994:Recombinant Proteins; D014765:Viral Vaccines