Conserved gene structure and transcription factor sites in the human and mouse deoxycytidine kinase genes.
Deoxycytidine kinase (dCK) phosphorylates several anti-cancer and anti-viral nucleoside analogs. The enzyme is predominantly expressed in lymphoid tissues regulated by an unknown mechanism. We have cloned and sequenced the 20 kbp mouse dCK gene and approximately 1.7 kbp of the 5' flanking regions of both the human and mouse dCK genes. Five major inter-species conserved motifs were identified in the 5' region including the transcription initiation region, an SP1 site and two closely located putative octamer transcription factor sites. Luciferase reporter experiments showed that the human dCK 5' region efficiently initiated transcription but no tissue regulatory element could be identified.
Johansson
M
M
Division of Clinical Virology, Karolinska Institute, Huddinge University Hospital, S-141 86, Stockholm, Sweden.
Norda
A
A
Karlsson
A
A
D020121:5' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D017124:Conserved Sequence; D003842:Deoxycytidine Kinase / Q000235:genetics*; D017930:Genes, Reporter; D006367:HeLa Cells; D006801:Humans; D019169:Jurkat Cells; D008156:Luciferases / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D013045:Species Specificity; D014157:Transcription Factors / Q000378:metabolism*; D015533:Transcriptional Activation; D014162:Transfection
D020121:5' Untranslated Regions; D014157:Transcription Factors; D008156:Luciferases; D003842:Deoxycytidine Kinase