Influence of the presence of OB-Re on leptin radioimmunoassay.
Leptin, a hormone derived from adipose tissue, regulates energy homeostasis and body weight. In the mouse, serum leptin levels, when measured by radioimmunoassay (RIA), increase by a factor of more than 50 times during pregnancy, compared with those in the non-pregnant state. It is well known that mouse placenta produces the secretory isoform of the leptin receptor, OB-Re. In order to investigate the issue of whether serum leptin levels are actually increased during pregnancy or whether the increased OB-Re concentration plays a role in this phenomenon, serum leptin levels were determined by the immunoprecipitation of leptin using anti-leptin antibody, and were found to be increased only by about ten times during pregnancy. To investigate the influence of OB-Re on leptin measurement by the RIA procedure, serum leptin levels were measured by the RIA after the addition of OB-Re to the serum. The apparent values of leptin levels increased in parallel with the amount of OB-Re added to the serum. Leptin levels, as determined by the RIA, might therefore provide artificially high values when serum levels of the secretory form of OB-R are high, in cases, for example, such as the last period of pregnancy in mice.
Murakami
T
T
Department of Laboratory Medicine, School of Medicine, University of Tokushima, Kuramotocho 3-chome, Tokushima 770-8503, Japan.
Otani
S
S
Honjoh
T
T
Doi
T
T
Shima
K
K
D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D002352:Carrier Proteins / Q000097:blood*; D005260:Female; D006207:Half-Life; D020738:Leptin / Q000097:blood*; D051379:Mice; D008813:Mice, Inbred ICR; D011233:Precipitin Tests; D011247:Pregnancy; D020033:Protein Isoforms; D011863:Radioimmunoassay; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; D012680:Sensitivity and Specificity
D002352:Carrier Proteins; D020738:Leptin; D020033:Protein Isoforms; D011956:Receptors, Cell Surface; D054411:Receptors, Leptin; C515447:leptin receptor, mouse