Archaeal promoter-directed expression of the Halobacterium salinarum catalase-peroxidase gene.
The Halobacterium salinarum catalase-peroxidase gene was subcloned into shuttle vectors pWL102 and pWL202 and expressed under the control of different archaeal promoters. When Hbt. salinarum was transformed with the catalase-peroxidase gene under the control of its own promoter, catalase-peroxidase activity increased twofold. Catalase-peroxidase activity increased threefold when Hbt. salinarum was transformed with the catalase-peroxidase gene under the control of a tRNA promoter. This bifunctional enzyme in Hbt. salinarum was not induced by environmental stresses such as H2O2, intense light, darkness, high temperature, low temperature, redox inhibitors, heavy metals, or ions.
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Department of Biochemistry and Molecular Biology, Mississippi State University, 39762, USA. mls1@ra.msstate.edu
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D001483:Base Sequence; D002374:Catalase / Q000096:biosynthesis / Q000235:genetics*; D017931:DNA Primers; D004790:Enzyme Induction; D019848:Gene Expression Regulation, Archaeal / Q000235:genetics*; D006217:Halobacterium / Q000201:enzymology / Q000235:genetics*; D018384:Oxidative Stress; D010544:Peroxidases / Q000096:biosynthesis / Q000235:genetics*; D011401:Promoter Regions, Genetic
D017931:DNA Primers; D010544:Peroxidases; D002374:Catalase