Cytomegalovirus infection in renal transplant recipients diagnosed by nested-PCR.
A prospective study of cytomegalovirus (CMV) infection was carried out on 34 renal transplant recipients managed at a General Hospital in Ribeirão Preto, SP, Brazil. Serologic tests showed that all patients were infected with CMV before renal transplantation. Two nested-PCR techniques with primers that recognize sequences of the glycoprotein B (gB) and H (gH) genes were used for CMV detection in blood and urine samples during the post-transplantation period. CMV was detected more frequently in blood samples than in urine samples (P<0.001). Thirty-three patients had CMV detected at least once in blood and/or urine samples. Seven of these patients (21.2%) were diagnosed as having symptomatic CMV infection and showed a worse clinical outcome, with a higher death rate (P = 0.03). No association between CMV viremia and graft rejection was observed. Nested-PCR was not useful to identify patients at risk for symptomatic CMV infection since only 21.2% of the patients with CMV infection were symptomatic.
Aquino
V H
VH
Unidade Multidepartamental de Pesquisa em Virologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.
Figueiredo
L T
LT
D000914:Antibodies, Viral / Q000302:isolation & purification; D001483:Base Sequence; D003586:Cytomegalovirus Infections / Q000097:blood / Q000175:diagnosis* / Q000652:urine; D017931:DNA Primers; D006801:Humans; D007074:Immunoglobulin G / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000302:isolation & purification; D016030:Kidney Transplantation; D016133:Polymerase Chain Reaction / Q000379:methods*; D011446:Prospective Studies; D014759:Viral Envelope Proteins / Q000235:genetics
D000914:Antibodies, Viral; D017931:DNA Primers; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus; C044200:glycoprotein H, herpes simplex virus type 1