Low-frequency stimulation of fast muscle affects the abundance of Ca(2+)-ATPase but not its oligomeric status.
After chronic, low-frequency stimulation, a rapid decline in Ca(2+) pump activity is observed during the early stages of skeletal muscle transformation. However, this variation in enzymatic activity does not coincide with a drastic reduction in the amount of sarcoplasmic reticulum Ca(2+)-ATPases. To investigate whether changes in subunit interactions within Ca(2+) pump complexes contribute to this phenomena, we performed a chemical cross-linking analysis of 4 days continuously, and 4 days discontinuously, electrostimulated fast muscle fibers. The abundance of the slow and fast Ca(2+)-ATPase isoforms sarco(endo)plasmic reticulum Ca(2+)- ATPase types 1 and 2 was affected during the fast-to-slow transition process, demonstrating that, even after short-term stimulation, distinct changes in the isoform expression pattern of muscle proteins occur. However, the oligomeric status of both ion pump species did not change. Hence, chemical modifications of critical enzyme domains must be responsible for the rapid stimulation-induced activity changes, not variations in protein-protein interactions within Ca(2+)-ATPase units. Oligomerization appears to be of central importance to the proper physiological functioning of the Ca(2+)-ATPase and does not undergo changes during skeletal muscle conditioning.
Harmon
S
S
Department of Pharmacology, Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland.
Froemming
G R
GR
Leisner
E
E
Pette
D
D
Ohlendieck
K
K
D000818:Animals; D000252:Calcium-Transporting ATPases / Q000737:chemistry* / Q000378:metabolism*; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004558:Electric Stimulation / Q000379:methods; D008297:Male; D018656:Muscle Fibers, Fast-Twitch / Q000502:physiology*; D018482:Muscle, Skeletal / Q000201:enzymology* / Q000502:physiology; D011108:Polymers / Q000378:metabolism; D011817:Rabbits; D012146:Rest; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases
D003432:Cross-Linking Reagents; D011108:Polymers; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D000252:Calcium-Transporting ATPases