title: Developments in the measurement of plasma total homocysteine.
abstract: Elevated plasma homocysteine is considered to be a graded risk factor for cardiovascular disease, and for this reason there is great interest in high-performance analytical techniques. Methods have evolved from ion-exchange chromatography to embrace high-performance liquid chromatography with fluorescence or electrochemical detection, immunoassays, gas chromatography-mass spectroscopy and liquid chromatography with tandem mass spectrometry. Immunoassays and high-performance liquid chromatography methods are currently available in kit form, fluorescence polarization immunoassay showing the best performance.
authors.affiliation: The Centre for Human Nutrition, Division of Clinical Sciences, University of Sheffield, Sheffield, UK. h.j.powers@sheffield.ac.uk
authors.forename: H J
authors.initials: HJ
authors.lastname: Powers
authors.forename: S J
authors.initials: SJ
authors.lastname: Moat
mesh_terms: D002318:Cardiovascular Diseases / Q000097:blood / Q000175:diagnosis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D012307:Risk Factors; D013050:Spectrometry, Fluorescence
chemical_list: D006710:Homocysteine