title: Identification of a cluster of strains bearing a new adhesin among genetically diverse enterotoxigenic Escherichia coli isolates of serogroup O20.
abstract: About one-third of the enterotoxigenic Escherichia coli isolates lack any of the known colonization factors. Among this group, those of serogroup O20 are the most frequently isolated in Argentina. By combining analysis of adhesion to Caco-2 cells, random amplified polymorphic DNA, and pulsed-field gel electrophoresis techniques, we were able to identify three sets of closely related strains with different binding properties. Further analysis of the most prevalent group revealed that all these isolates expressed the recently described adhesin CS22.
authors.affiliation: Departamento de Bacteriología, Instituto Nacional de Enfermedades Infecciosas-ANLIS Carlos G. Malbrán, Buenos Aires, Argentina.
authors.forename: M
authors.initials: M
authors.lastname: Pichel
authors.forename: N
authors.initials: N
authors.lastname: Binsztein
authors.forename: G
authors.initials: G
authors.lastname: Gutkind
authors.forename: G
authors.initials: G
authors.lastname: Viboud
mesh_terms: D018830:Adhesins, Escherichia coli / Q000235:genetics*; D001118:Argentina; D001427:Bacterial Toxins / Q000235:genetics; D002648:Child; D016000:Cluster Analysis; D003967:Diarrhea / Q000382:microbiology; D016521:Electrophoresis, Gel, Pulsed-Field; D004768:Enterotoxins / Q000235:genetics; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology; D029968:Escherichia coli Proteins; D014644:Genetic Variation; D006801:Humans; D010641:Phenotype; D010802:Phylogeny; D019105:Random Amplified Polymorphic DNA Technique; D012703:Serotyping; D013045:Species Specificity; D014407:Tumor Cells, Cultured
chemical_list: D018830:Adhesins, Escherichia coli; D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C034151:heat stable toxin (E coli); C027538:heat-labile enterotoxin, E coli