Effect of APGW-amide on [Ca2+]i in rat pheochromocytoma PC12 cells.
In order to determine whether Ala-Pro-Gly-Try-NH2 (APGW-amide) could affect mammalian excitable cells, we investigated the effect of APGW-amide in PC12 cells. APGW-amide caused a rapid [Ca2+]i elevation, which was completely prevented by elimination of extracellular Ca2+ with EGTA and inhibited by two L-type Ca2+ channel blockers. [Ca2+]i elevation was also blocked by a specific PKC inhibitor and prolonged pretreatment of cells with PMA. These results indicate that APGW-amide elevates [Ca2+]i in PC12 cells, possibly by Ca2+ influx via L-type Ca2+ channel activated by PKC.
Han
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XY
Department of Neurophysiology, Institute of Equilibrium Research, Gifu University School of Medicine, Japan.
Ito
Y
Y
Nozawa
Y
Y
Jiang
Y
Y
Matsunami
K
K
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000494:pharmacology; D020746:Calcium Channels, L-Type / Q000187:drug effects / Q000502:physiology; D020013:Calcium Signaling; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005456:Fluorescent Dyes; D016257:Fura-2; D015640:Ion Channel Gating; D009479:Neuropeptides / Q000494:pharmacology*; D016716:PC12 Cells; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D051381:Rats
D002121:Calcium Channel Blockers; D020746:Calcium Channels, L-Type; D004791:Enzyme Inhibitors; D005456:Fluorescent Dyes; D009479:Neuropeptides; C063193:alanyl-prolyl-glycyl-tryptophanamide; D011493:Protein Kinase C; D002118:Calcium; D016257:Fura-2