In vivo study of percutaneous absorption of 4-chloroaniline using microdialysis in the rat.
The present work was undertaken to study the percutaneous absorption of 4-chloroaniline (parachloroaniline, PCPA, CAS 106-47-8), a chemical intermediate in pesticide manufacture, using in vivo microdialysis in the rat. The results of the microdialysis study showed that PCPA was able to cross the skin (Tmax = 3 h and area under the curve (AUC) = 332.1 ng.h/ml) and rapidly enter the systemic circulation (Tmax = 3.3 +/- 0.6 h). It could be also shown that PCPA was partly metabolised during its percutaneous absorption. The analysis of cutaneous dialysate samples using gas chromatography/mass spectrometry (GC-MS) showed that the metabolite was 4-chloracetanilide. Taken together, the data obtained showed that contact with the skin is a danger for exposed persons.
el Marbouh
L
L
Laboratoire de Cinétique des Xénobiotiquès, France.
Arellano
C
C
Philibert
C
C
Evrard
P
P
Poëy
J
J
Houin
G
G
D000107:Acetylation; D000814:Aniline Compounds / Q000008:administration & dosage / Q000493:pharmacokinetics*; D000818:Animals; D019540:Area Under Curve; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006207:Half-Life; D017551:Microdialysis; D051381:Rats; D012867:Skin / Q000737:chemistry; D012869:Skin Absorption
D000814:Aniline Compounds; C004658:4-chloroaniline