title: Primary reactions of sensory rhodopsins.
abstract: The first steps in the photocycles of the archaeal photoreceptor proteins sensory rhodopsin (SR) I and II from Halobacterium salinarum and SRII from Natronobacterium pharaonis have been studied by ultrafast pump/probe spectroscopy and steady-state fluorescence spectroscopy. The data for both species of the blue-light receptor SRII suggests that their primary reactions are nearly analogous with a fast decay of the excited electronic state in 300-400 fs and a transition between two red-shifted product states in 4-5 ps. Thus SRII behaves similarly to bacteriorhodopsin. In contrast for SRI at pH 6.0, which absorbs in the orange part of the spectrum, a strongly increased fluorescence quantum yield and a drastically slower and biexponential decay of the excited electronic state occurring on the picosecond time scale (5 ps and 33 ps) is observed. The results suggest that the primary reactions are controlled by the charge distribution in the vicinity of the Schiff base and demonstrate that there is no direct connection between absorption properties and reaction dynamics for the retinal protein family.
authors.affiliation: Sektion Physik, Ludwig-Maximilians-Universität München, Oettingenstrasse 67, 80538 Munich, Germany.
authors.forename: I
authors.initials: I
authors.lastname: Lutz
authors.forename: A
authors.initials: A
authors.lastname: Sieg
authors.forename: A A
authors.initials: AA
authors.lastname: Wegener
authors.forename: M
authors.initials: M
authors.lastname: Engelhard
authors.forename: I
authors.initials: I
authors.lastname: Boche
authors.forename: M
authors.initials: M
authors.lastname: Otsuka
authors.forename: D
authors.initials: D
authors.lastname: Oesterhelt
authors.forename: J
authors.initials: J
authors.lastname: Wachtveitl
authors.forename: W
authors.initials: W
authors.lastname: Zinth
mesh_terms: D019843:Archaeal Proteins; D001436:Bacteriorhodopsins / Q000737:chemistry* / Q000378:metabolism*; D002338:Carotenoids; D019617:Halobacterium salinarum / Q000378:metabolism; D025602:Halorhodopsins; D007700:Kinetics; D019633:Natronobacterium / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D025603:Sensory Rhodopsins; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D013997:Time Factors
chemical_list: D019843:Archaeal Proteins; D025602:Halorhodopsins; D011994:Recombinant Proteins; C061497:SRI protein, Halobacterium; D025603:Sensory Rhodopsins; C061508:sensory rhodopsin II protein, archaeal; D002338:Carotenoids; D001436:Bacteriorhodopsins