Reduction of vesicular acetylcholine transporter in beta-amyloid protein-infused rats with memory impairment.
The aim of this study was to investigate spatial memory and quantitative acetylcholine transporter autoradiography using a high-sensitivity imaging plate system in rats treated with beta-amyloid protein, a model of Alzheimer's disease. An eight-arm radial maze was used to evaluate spatial memory. The performance of the eight-arm radial maze task was impaired in beta-amyloid protein-treated rats. In the parietal cortex, [3H]-vesamicol binding to the vesicular acetylcholine transporter was significantly lower in beta-amyloid protein-treated rats than in vehicle-treated rats, and was significantly correlated with the mean number of correct selections in the maze task of the first 5 days in the post-operative state. These results indicate that the reduction in [3H]-vesamicol binding to vesicular acetylcholine transporter is related to memory impairment induced by beta-amyloid protein. Beta-amyloid protein-infused rats with spatial memory impairment may be useful for the development of new radiolabelled vesamicol analogues for the objective evaluation of Alzheimer's disease.
Ikeda
E
E
Department of Nuclear Medicine, Kanazawa University School of Medicine, Japan.
Shiba
K
K
Mori
H
H
Ichikawa
A
A
Sumiya
H
H
Kuji
I
I
Tonami
N
N
D000544:Alzheimer Disease / Q000000981:diagnostic imaging / Q000378:metabolism; D016229:Amyloid beta-Peptides / Q000008:administration & dosage*; D000818:Animals; D001345:Autoradiography; D002352:Carrier Proteins / Q000378:metabolism*; D004195:Disease Models, Animal; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D026901:Membrane Transport Proteins; D008569:Memory Disorders / Q000139:chemically induced / Q000000981:diagnostic imaging* / Q000378:metabolism*; D010880:Piperidines / Q000378:metabolism; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals; D051381:Rats; D017207:Rats, Sprague-Dawley; D011976:Receptors, Muscarinic / Q000378:metabolism; D013572:Synaptic Vesicles / Q000378:metabolism; D014018:Tissue Distribution; D014316:Tritium; D050494:Vesicular Acetylcholine Transport Proteins; D033921:Vesicular Transport Proteins
D016229:Amyloid beta-Peptides; D002352:Carrier Proteins; D026901:Membrane Transport Proteins; D010880:Piperidines; D019275:Radiopharmaceuticals; D011976:Receptors, Muscarinic; C493459:SLC18A3 protein, human; C493461:Slc18a3 protein, rat; D050494:Vesicular Acetylcholine Transport Proteins; D033921:Vesicular Transport Proteins; D014316:Tritium; C006189:vesamicol; D011813:Quinuclidinyl Benzilate