Evaluation of in vitro methods for detecting the effects of various chemicals on the human progesterone receptor, with a focus on pyrethroid insecticides.
The progesterone receptor (PR) is associated with physiological events such as implantation and the maintenance of pregnancy. Recently, it has become a social concern that chemicals may exert agonistic or antagonistic effects on hormone receptors. Therefore, we examined the effects of various chemicals on the human PR, with a focus on pyrethroid insecticides, using three in vitro methods. Eight pyrethroid insecticides (fenvalerate, d-allethrin, d-phenothrin, prallethrin, empenthrin, permethrin, cypermethrin and imiprothrin), examples of environmental pollutants and positive control chemicals were subjected to a reporter gene assay (luciferase assay) using human breast cancer T-47D cells, a two-hybrid assay and a binding assay using the same whole cells or receptors (cell-free). In none of these did the eight pyrethroid insecticides show any binding to the PR, agonistic or antagonistic effects.
Sumida
K
K
Environmental Health Science Laboratory, Sumitomo Chemical Co. Ltd., 1-98, 3-Chome, Kasugade-Naka, Konohana-ku, 554-8558, Osaka, Japan. sumida@sc.sumitomo-chem.co.jp
Saito
K
K
Ooe
N
N
Isobe
N
N
Kaneko
H
H
Nakatsuka
I
I
D001667:Binding, Competitive; D002474:Cell-Free System; D017930:Genes, Reporter; D006801:Humans; D007306:Insecticides / Q000378:metabolism / Q000633:toxicity*; D007700:Kinetics; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics; D011722:Pyrethrins / Q000378:metabolism / Q000633:toxicity*; D011980:Receptors, Progesterone / Q000819:agonists* / Q000037:antagonists & inhibitors* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured
D007306:Insecticides; D011722:Pyrethrins; D011980:Receptors, Progesterone; D008156:Luciferases