Immunohistochemical localization of adenosine A1 receptors in human brain regions.
Adenosine exerts its physiological actions by binding to G-protein coupled receptors, four of which have been identified and cloned to date (A1, A2a, A2b and A3). Here we report the development of anti-human adenosine A1, receptor anti-peptide polyclonal antibodies and their use to define the distribution of A1, receptors in human brain regions, spinal cord and trigeminal ganglia by an immunohistochemical approach. Although the distribution of adenosine A1, receptor and its mRNA in the human brain has been investigated in the past by autoradiography and in situ hybridization, this is the first demonstration of localization of the A1, receptors by immunohistochemical means. Our localization data broadly agree with immunohistochemical data published for the human brain obtained using other experimental approaches. Furthermore, we have demonstrated the novel finding that abundant expression of the adenosine A1, receptor protein occurs in the trigeminal ganglia, which may be suggestive of a role of this receptor in analgesia.
Schindler
M
M
Department of Pharmacology, Glaxo Institute of Applied Pharmacology, University of Cambridge, Tennis Court Road, CB2 I QJ, Cambridge, United Kingdom. marcus.schindler@bc.boehringer-ingelheim.com
Harris
C A
CA
Hayes
B
B
Papotti
M
M
Humphrey
P P
PP
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000918:Antibody Specificity; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D016466:CHO Cells; D006224:Cricetinae; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D018047:Receptors, Purinergic P1 / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism* / Q000473:pathology; D012668:Trigeminal Ganglion / Q000378:metabolism* / Q000473:pathology
D018047:Receptors, Purinergic P1