An immunogold investigation of the relationship between the amino acids GABA and glycine and their transporters in terminals in the guinea-pig anteroventral cochlear nucleus.
The majority of terminals contacting spherical bushy cell bodies in the guinea-pig anteroventral cochlear nucleus contain GABA, glycine or both (colocalizing). Double labeling with antibodies to each amino acid and the plasma membrane transporter for the other was performed using different sizes of gold particles. The transporter for GABA occurs in the plasma membranes of some terminals containing glycine and vice versa suggesting that colocalizing terminals can retrieve both amino acids.
Mahendrasingam
S
S
MacKay Institute of Communication and Neuroscience, School of Life Sciences, Keele University, Staffordshire ST5 5BG, UK.
Wallam
C A
CA
Hackney
C M
CM
D026921:Amino Acid Transport Systems, Neutral; D000818:Animals; D002352:Carrier Proteins / Q000032:analysis*; D002462:Cell Membrane / Q000648:ultrastructure; D017626:Cochlear Nucleus / Q000648:ultrastructure*; D050485:GABA Plasma Membrane Transport Proteins; D005998:Glycine / Q000032:analysis*; D050487:Glycine Plasma Membrane Transport Proteins; D006168:Guinea Pigs; D008565:Membrane Proteins / Q000032:analysis*; D026901:Membrane Transport Proteins; D016253:Microscopy, Immunoelectron / Q000379:methods; D009411:Nerve Endings / Q000648:ultrastructure*; D009474:Neurons / Q000648:ultrastructure*; D027361:Organic Anion Transporters; D005680:gamma-Aminobutyric Acid / Q000032:analysis*
D026921:Amino Acid Transport Systems, Neutral; D002352:Carrier Proteins; D050485:GABA Plasma Membrane Transport Proteins; D050487:Glycine Plasma Membrane Transport Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D027361:Organic Anion Transporters; D005680:gamma-Aminobutyric Acid; D005998:Glycine