Role of transcriptional factor activation protein-1 in endogenous expression of the interleukin-1 beta gene involved in Porphyromonas gingivalis fimbria-stimulated bone resorption in the mouse calvarial system.
We previously suggested that endogenous interleukin-1 beta (IL-1 beta) produced by Porphyromonas gingivalis fimbriae stimulation in calvarial bone cell cultures plays a role in bone resorption as a major cytokine. Therefore, in the present study, we initiated experiments to clarify the stimulatory mechanism of IL-1 beta gene expression in fimbria-stimulated bone resorption. Fimbria-stimulated bone resorption was dramatically inhibited by curcumin, a potent inhibitor of activation protein-1 (AP-1). In fact, the fimbriae induced markedly both the expression of c-fos and c-jun genes and their protein production in the calvarial cells. In addition, a mixture of antisense oligonucleotides against c-fos and c-jun significantly inhibited not only the fimbria-induced expression of the IL-1 beta gene but also the fimbria-induced bone resorption. Therefore, the present study demonstrates that transcriptional factor AP-1 plays a functional role in P. gingivalis fimbria-stimulated bone resorption via endogenous IL-1 beta in the mouse calvarial system.
Naganuma
K
K
Department of Oral Microbiology, Meikai University School of Dentistry, Keyakidai 1-1, Sakado City, Saitama 350-0283, Japan.
Amano
S
S
Takeda
H
H
Kitano
S
S
Hanazawa
S
S
D000818:Animals; D001862:Bone Resorption / Q000382:microbiology*; D003474:Curcumin / Q000494:pharmacology; D010861:Fimbriae, Bacterial / Q000502:physiology*; D015870:Gene Expression; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D016376:Oligonucleotides, Antisense; D016966:Porphyromonas gingivalis / Q000502:physiology*; D016760:Proto-Oncogene Proteins c-fos / Q000037:antagonists & inhibitors; D016755:Proto-Oncogene Proteins c-jun / Q000037:antagonists & inhibitors; D018808:Transcription Factor AP-1 / Q000037:antagonists & inhibitors / Q000502:physiology*
D007375:Interleukin-1; D016376:Oligonucleotides, Antisense; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D018808:Transcription Factor AP-1; D003474:Curcumin