title: A time-resolved fluoroimmunometric assay for the detection of microcystins, cyanobacterial peptide hepatotoxins.
abstract: An immunoassay based on the time-resolved fluorometry (TR-FIA) was developed for microcystins, cyanobacterial peptide hepatotoxins. The assay was performed in a competitive mode and it utilised the monoclonal antibodies raised against microcystin-LR, and a europium chelate of microcystin-LR as a competitive antigen. The sensitivity of the assay was 0.1microg/l. The detection method of TR-FIA was compared to a commercially available kit based on the enzyme-linked immunosorbent assay (ELISA). The same level of sensitivity could be obtained with TR-FIA (in a non-optimised system). The simplified method of TR-FIA leads to a shorter analysis time.
authors.affiliation: Department of Biochemistry and Pharmacy, Abo Akademi University, P.O. Box 66, 20521 Turku, Finland.
authors.forename: P
authors.initials: P
authors.lastname: Mehto
authors.forename: M
authors.initials: M
authors.lastname: Ankelo
authors.forename: A
authors.initials: A
authors.lastname: Hinkkanen
authors.forename: A
authors.initials: A
authors.lastname: Mikhailov
authors.forename: J E
authors.initials: JE
authors.lastname: Eriksson
authors.forename: L
authors.initials: L
authors.lastname: Spoof
authors.forename: J
authors.initials: J
authors.lastname: Meriluoto
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D002851:Chromatography, High Pressure Liquid; D000458:Cyanobacteria / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D008387:Marine Toxins; D052998:Microcystins; D010456:Peptides, Cyclic / Q000032:analysis* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D008387:Marine Toxins; D052998:Microcystins; D010456:Peptides, Cyclic; C057862:cyanoginosin LR