Biochemical characterization of profilin from seeds of Phaseolus vulgaris L.
The isoform composition of the 14.4 kDa profilin polypeptide was analyzed in seeds, leaves, flowers, roots and root-nodules from Phaseolus vulgaris L. Isoforms of pIs approximately 4.4-5 were present in all the tissues analyzed. The biochemical features of the protein present in seed tissue were determined. Seed profilin bound to Phenyl-Sepharose under low salt conditions which suggested a hydrophobic interaction; however, it was not associated with microsomal membranes nor it partitioned as a hydrophobic protein in Triton X-114. Fractions eluting from poly-L-proline or Phenyl-Sepharose columns contained well detectable amounts of profilin but no actin, suggesting that most of the protein was not present as profilactin in the seed. However, seed profilin appeared to be in some kind of complex since several molecular weight species were observed on native gels. In addition, profilin was found preferentially in the embryo axis and light microscopic immunolocalization showed a cytoplasmic distribution in this tissue.
Guillén
G
G
Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, U.N.A.M. Apartado Postal 510-3, Cuernavaca, Morelos 62250, México.
López-Sánchez
L M
LM
Román-Roque
C S
CS
Sánchez
F
F
Villanueva
M A
MA
D015153:Blotting, Western; D002847:Chromatography, Agarose; D003285:Contractile Proteins; D003593:Cytoplasm / Q000378:metabolism; D004586:Electrophoresis; D007887:Fabaceae / Q000737:chemistry* / Q000378:metabolism; D066298:In Vitro Techniques; D008840:Microfilament Proteins / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D008854:Microscopy, Electron; D008861:Microsomes / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry; D010940:Plant Proteins / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal; D051304:Profilins; D020033:Protein Isoforms / Q000032:analysis / Q000737:chemistry; D012639:Seeds / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure
D003285:Contractile Proteins; D008840:Microfilament Proteins; D010940:Plant Proteins; D051304:Profilins; D020033:Protein Isoforms