title: Sepsis increases the plasma membrane content of alpha1 and alpha2 isoforms of Na+-K+ adenosine triphosphatase in rat skeletal muscle.
abstract: OBJECTIVE
  Increased Na(+)-K(+) adenosine triphosphatase (ATPase) activity in skeletal muscle during sepsis is caused by transient increases in enzyme content within the plasma membrane.
  
  
  METHODS
  Randomized controlled study.
  
  
  METHODS
  University laboratory.
  
  
  METHODS
  Eighty-eight adult male Wistar rats were randomly assigned to undergo cecal ligation and puncture (CLP) or sham operation.
  
  
  METHODS
  Gastrocnemius muscles were harvested 6, 12, 24, and 48 hours after operation and Na(+)-K(+) ATPase activities were measured spectrofluorimetrically. Messenger RNA (mRNA) levels for the alpha1 and alpha2 isoforms of Na(+)-K(+) ATPase were determined by Northern blot analysis. Crude membranes, internal membranes, and purified plasma membranes were isolated from gastrocnemius muscles and protein levels of alpha1 and alpha2 isoforms were determined by Western blot analysis.
  
  
  RESULTS
  Na(+)-K(+) ATPase activity in the CLP group was significantly higher compared with the sham group 24 hours after operation (P<.05). However, there were no differences between the sham and CLP groups 6, 12, or 48 hours after operation. No significant differences between the CLP and sham groups were noted in mRNA levels for Na(+)-K(+) ATPase alpha1 and alpha2 isoforms. Western blot analysis revealed that the plasma membrane (but not internal membrane or crude membrane) content of alpha2 and alpha1 isoforms from the CLP group was significantly increased compared with the sham group 24 hours after operation (P<.05).
  
  
  CONCLUSIONS
  Na(+)-K(+) ATPase activity increases 24 hours after CLP in gastrocnemius muscle and then declines. This increase is caused by increased Na(+)-K(+) ATPase protein levels in the plasma membrane.
authors.affiliation: Department of Surgery, Creighton University, 601 North 30th St, Suite 3740, Omaha, NE 68131, USA.
authors.forename: N
authors.initials: N
authors.lastname: Shimoda
authors.forename: J
authors.initials: J
authors.lastname: Jasleen
authors.forename: J D
authors.initials: JD
authors.lastname: Rounds
authors.forename: S W
authors.initials: SW
authors.lastname: Ashley
authors.forename: D O
authors.initials: DO
authors.lastname: Jacobs
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002432:Cecum / Q000601:surgery; D002462:Cell Membrane / Q000201:enzymology*; D007527:Isoenzymes; D008297:Male; D018482:Muscle, Skeletal / Q000201:enzymology*; D012333:RNA, Messenger / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000201:enzymology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000737:chemistry / Q000378:metabolism*; D013997:Time Factors
chemical_list: D007527:Isoenzymes; D012333:RNA, Messenger; D000254:Sodium-Potassium-Exchanging ATPase