Loss of heterozygosity in DNA mismatch repair genes in human atherosclerotic plaques.
To detect the incidence of loss of heterozygosity (LOH) in DNA mismatch repair genes (MMR) occurring in atherosclerosis, fifty human autopsy cases of atherosclerosis were examined for LOH using 19 microsatellite markers, in three single and four tetraplex microsatellite assays. The markers used are located on or close to MMR genes. Fourteen specimens (28%) showed allelic imbalance in at least one locus. Loci hMSH2 (2p22.3-p16.1), hPMS1 (2q24.1-q32.1), and hMLH1 (3p21.32-p21.1) exhibited LOH (10, 10, and 12% respectively). We found that loss of heterozygosity on hMSH2, hPMS1, and hMLH1, occurs in atherosclerosis. The occurrence of such genomic alterations may represent important events in the development of atherosclerosis.
Flouris
G A
GA
Department of Virology, Medical School, Heraklion, Crete, Greece.
Arvanitis
D A
DA
Parissis
J T
JT
Arvanitis
D L
DL
Spandidos
D A
DA
D000368:Aged; D001161:Arteriosclerosis / Q000235:genetics* / Q000473:pathology; D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2; D002893:Chromosomes, Human, Pair 3; D004260:DNA Repair / Q000235:genetics*; D005260:Female; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D008875:Middle Aged; D009692:Nucleic Acid Heteroduplexes / Q000235:genetics*; D016133:Polymerase Chain Reaction
D009692:Nucleic Acid Heteroduplexes