Simultaneous spectrophotometric determination of phenobarbital, phenytoin and methylphenobarbital in pharmaceutical preparations by using partial least-squares and principal component regression multivariate calibration.
Two multivariate calibration methods, partial least squares (PLS-2) and principal component regression (PCR) have been applied to the simultaneous spectrophotometric analysis of ternary mixtures of phenytoin (DPH), phenobarbital (PBT) and methylphenobarbital (MPBT) in the Comital-L pharmaceutical formulation. The PLS-2 and PCR procedures were employed to evaluate the data of a variable number of calibration solutions measured over the wavelength range 400-700 nm. The concentration ranges used to construct the calibration matrix were varied between 5 and 30 microg ml(-1). The proposed methods were validated by applying them to the analysis of the Comital-L pharmaceutical formulation and the average relative errors were less than 6% for each one of the analyzed compounds. The results obtained by both proposed methods have been compared with the results obtained by application of a RPLC reference method.
Boeris
Departameto de Química, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de La Pampa, Universidad Nacional de San Luis, Chacabuco y Pedernera, Argentina.
Luco
J M
JM
Olsina
R A
RA
D016018:Least-Squares Analysis; D008618:Mephobarbital / Q000032:analysis*; D015999:Multivariate Analysis; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D010634:Phenobarbital / Q000032:analysis*; D010672:Phenytoin / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D004364:Pharmaceutical Preparations; D008618:Mephobarbital; D010672:Phenytoin; D010634:Phenobarbital