title: Acute pyelonephritis: comparison of diagnosis with 99mTc-DMSA, SPECT, spiral CT, MR imaging, and power Doppler US in an experimental pig model.
abstract: OBJECTIVE
  To compare the sensitivity and specificity of technetium-99m dimercaptosuccinic acid (DMSA) single photon emission computed tomography (SPECT), spiral computed tomography (CT), magnetic resonance (MR) imaging, and power Doppler ultrasonography (US) for the detection and localization of acute pyelonephritis by using histopathologic findings as the standard of reference.
  
  
  METHODS
  Bilateral vesicoureteric reflux was surgically created in 35 piglets (70 kidneys). One week later, a liquid bacterial culture of Escherichia coli was injected into the bladder. Three days after induction of urinary infection, imaging studies were performed, and the kidneys were removed for histopathologic examination. SPECT images were obtained 2-3 hours after injection of 99mTc-DMSA. Transverse and coronal MR images were obtained with gadolinium-enhanced fast inversion recovery. Transverse CT images were obtained before and after injection of contrast agent. Power Doppler US was performed in longitudinal, transverse, and coronal planes. Each kidney was divided into three zones for correlation of findings.
  
  
  RESULTS
  Histopathologic examination revealed pyelonephritis in 102 zones in 38 kidneys. Sensitivity and specificity for detecting pyelonephritis in the kidneys were 92.1% and 93.8% for SPECT, 89.5% and 87.5% for MR imaging, 86.8% and 87.5% for CT, and 74.3% and 56.7% for US. Sensitivity and specificity for detecting pyelonephritis in the zones were 94.1% and 95.4% for SPECT, 91.2% and 92.6% for MR imaging, 88.2% and 93.5% for CT, and 56.6% and 81.4% for US. The pairwise comparison of these modalities showed no statistically significant difference among them except for US.
  
  
  CONCLUSIONS
  99mTc-DMSA SPECT, spiral CT, and MR imaging appear to be equally sensitive and reliable for the detection of acute pyelonephritis; power Doppler US is significantly less accurate.
authors.affiliation: Dept of Radiology, Children's National Medical Center and the George Washington Univ School of Medicine, 111 Michigan Ave NW, Washington, DC 20010, USA. mmajd@cnmc.org
authors.forename: M
authors.initials: M
authors.lastname: Majd
authors.forename: A R
authors.initials: AR
authors.lastname: Nussbaum Blask
authors.forename: B M
authors.initials: BM
authors.lastname: Markle
authors.forename: E
authors.initials: E
authors.lastname: Shalaby-Rana
authors.forename: H G
authors.initials: HG
authors.lastname: Pohl
authors.forename: J S
authors.initials: JS
authors.lastname: Park
authors.forename: R
authors.initials: R
authors.lastname: Chandra
authors.forename: K
authors.initials: K
authors.lastname: Rais-Bahrami
authors.forename: N
authors.initials: N
authors.lastname: Pandya
authors.forename: K M
authors.initials: KM
authors.lastname: Patel
authors.forename: H G
authors.initials: HG
authors.lastname: Rushton
mesh_terms: D000208:Acute Disease; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D008279:Magnetic Resonance Imaging; D008297:Male; D011704:Pyelonephritis / Q000175:diagnosis*; D019275:Radiopharmaceuticals; D012680:Sensitivity and Specificity; D013552:Swine; D019783:Technetium Tc 99m Dimercaptosuccinic Acid; D015899:Tomography, Emission-Computed, Single-Photon; D014057:Tomography, X-Ray Computed / Q000379:methods; D018608:Ultrasonography, Doppler
chemical_list: D019275:Radiopharmaceuticals; D019783:Technetium Tc 99m Dimercaptosuccinic Acid