Cefpodoxime screening of Escherichia coli and Klebsiella spp. by Vitek for detection of organisms producing extended-spectrum beta-lactamases.
We used a Vitek custom card to detect cefpodoxime MIC of--2 mg/L as a screen for extended-spectrum beta-lactamase (ESBL) production in E. coli and Klebsiella spp. Of 2873 organisms tested, 60 were screen positive, but only 3 were confirmed to be ESBL producers. Cefpodoxime is believed to be a sensitive screen for ESBL production, but a more specific test is desirable.
Gibb
A P
AP
Microbiology Department, Calgary Laboratory Services, Calgary, Alberta, Canada T3C0J5. pgibb@freenet.co.uk
Crichton
M
M
D015296:Ceftizoxime / Q000031:analogs & derivatives* / Q000494:pharmacology*; D002511:Cephalosporins / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology*; D004927:Escherichia coli Infections / Q000382:microbiology; D006801:Humans; D007709:Klebsiella / Q000187:drug effects / Q000201:enzymology*; D007710:Klebsiella Infections / Q000382:microbiology; D008826:Microbial Sensitivity Tests / Q000592:standards; D012680:Sensitivity and Specificity; D001618:beta-Lactamases / Q000378:metabolism*; D000097623:Cefpodoxime
D015296:Ceftizoxime; D002511:Cephalosporins; D001618:beta-Lactamases; D000097623:Cefpodoxime