title: Nitric oxide interferes with islet cell zinc homeostasis.
abstract: Zinc is crucial for the biosynthesis, storage, and secretion of insulin in pancreatic islet cells. We have previously presented evidence that NO interferes with cellular Zn(2+) homeostasis and we therefore investigated the influence of chronic NO exposure on the labile islet cell Zn(2+) content. A strong fluorescence activity in a large islet cell subpopulation was found after staining with the Zn(2+)-specific fluorophore Zinquin. Culture for 24 h in the presence of nontoxic concentrations of the slow-releasing NO donor DETA/NO resulted in a significantly reduced Zn(2+)-dependent fluorescence. This appears to be islet specific as in endothelial cells DETA/NO exposure enhanced the Zn(2+)-dependent fluorescence activity in a concentration-dependent manner. These results suggest that NO interferes with cellular Zn(2+) homeostasis, which in islet cells is crucial for proper hormone delivery and thus special cell function.
authors.affiliation: Research Group Immunobiology, Medical Department, Heinrich-Heine-University, Düsseldorf, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Tartler
authors.forename: K D
authors.initials: KD
authors.lastname: Kröncke
authors.forename: K L
authors.initials: KL
authors.lastname: Meyer
authors.forename: C V
authors.initials: CV
authors.lastname: Suschek
authors.forename: V
authors.initials: V
authors.lastname: Kolb-Bachofen
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005434:Flow Cytometry; D005453:Fluorescence; D005456:Fluorescent Dyes; D006706:Homeostasis / Q000187:drug effects; D007515:Islets of Langerhans / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism* / Q000494:pharmacology; D020030:Nitric Oxide Donors / Q000494:pharmacology; D015363:Quinolones; D051381:Rats; D017208:Rats, Wistar; D014105:Tosyl Compounds; D014226:Triazenes / Q000494:pharmacology; D015032:Zinc / Q000378:metabolism*
chemical_list: C105351:1-hydroxy-2-oxo-3,3-bis(2-aminoethyl)-1-triazene; D005456:Fluorescent Dyes; D020030:Nitric Oxide Donors; D015363:Quinolones; D014105:Tosyl Compounds; D014226:Triazenes; D009569:Nitric Oxide; D015032:Zinc; C084731:zinquin