Orthopoxvirus IL-18 binding proteins: affinities and antagonist activities.
The affinities of purified recombinant human IL-18 binding protein (BP) and ectromelia and cowpox virus homologs for human and murine IL-18 were compared by plasmon resonance. The dissociation constants of human IL-18BP were similar for murine and human IL-18. By contrast, the dissociation constants of the viral proteins for murine IL-18 were 12- to 50-fold lower than that for human IL-18. The ectromelia and cowpox virus proteins were biologically active, as judged by their ability to inhibit induction of interferon-gamma by murine and human IL-18. The relative affinities of the orthopoxvirus IL-18BPs are consistent with the rodent host range of the viruses.
Calderara
S
S
Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, 20892-0445, USA.
Xiang
Y
Y
Moss
B
B
D000818:Animals; D002460:Cell Line; D015608:Cowpox virus / Q000235:genetics / Q000378:metabolism; D004481:Ectromelia virus / Q000235:genetics / Q000378:metabolism; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D007371:Interferon-gamma / Q000096:biosynthesis; D020382:Interleukin-18 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D018155:Orthopoxvirus / Q000235:genetics / Q000378:metabolism*; D011213:Poxviridae Infections / Q000821:virology; D011994:Recombinant Proteins / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000276:immunology; D020349:Surface Plasmon Resonance; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; D020382:Interleukin-18; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; C118370:interleukin-18 binding protein; D007371:Interferon-gamma