Yang-gan-wan protects mice against apoptosis induced by anti-Fas antibody (Jo2).
We investigated the protective effect of yang-gan-wan (YGW, Pro-Liver pill), a Chinese herbal remedy, against liver apoptosis (programmed cell death) induced by anti-Fas antibody (Jo2). Mice were pretreated daily with 200 mg/kg YGW for 14 days before treatment with 10 microg/20 kg body weight Jo2. YGW significantly reduced the elevated activity of alanine aminotransferase (ALT) and also reduced the elevated activity of sorbitol dehydrogenase (SDH) produced 4 hrs after treatment with Jo2. YGW also increased the survival of mice treated with Jo2 by about 1 hr in 11 out of 16 mice (69%). Most of the mice died 9 hrs after Jo2 injection. This study demonstrates that YGW has protective effects against liver apoptosis induced by Jo2.
Yang
M
M
Department of Molecular Pharmacology and Toxicology, School of Pharmacy, University of Southern California, Los Angeles 90089, USA.
Chen
K
K
Shih
J C
JC
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D007064:L-Iditol 2-Dehydrogenase / Q000097:blood; D008099:Liver / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D019014:fas Receptor / Q000276:immunology*
D004365:Drugs, Chinese Herbal; C415597:Yang-Gan-Wan; D019014:fas Receptor; D007064:L-Iditol 2-Dehydrogenase; D000410:Alanine Transaminase