Low-cost microbioreactor for high-throughput bioprocessing.
The design of a microbioreactor is described. An optical sensing system was used for continuous measurements of pH, dissolved oxygen, and optical density in a 2 mL working volume. The K(L)a of the microbioreactor was evaluated under different conditions. An Escherichia coli fermentation in both the microbioreactor and a standard 1 L bioreactor showed similar pH, dissolved oxygen, and optical density profiles.%The low cost of the microbioreactor, detection system, and the small volume of the fermentation broth provide a basis for development of a multiple-bioreactor system for high-throughput bioprocess optimization.
Kostov
Y
Y
Department of Chemical and Biochemical Engineering, University of Maryland Baltimore County, Baltimore, Maryland 21250, USA.
Harms
P
P
Randers-Eichhorn
L
L
Rao
G
G
D019149:Bioreactors / Q000191:economics*; D015374:Biosensing Techniques; D002138:Calibration; D003470:Culture Media / Q000737:chemistry; D004926:Escherichia coli / Q000254:growth & development; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010100:Oxygen / Q000032:analysis; D013053:Spectrophotometry
D003470:Culture Media; D010100:Oxygen