Rapid detection of mutations in the 23S rRNA gene of Helicobacter pylori that confers resistance to clarithromycin treatment to the bacterium.
We developed a new method capable of detecting point mutations in the 23S rRNA gene of Helicobacter pylori using a LightCycler. Our method can detect a mutation in this gene in less than 1 h and can process many samples at once, thereby contributing to the selection of patients suitable for clarithromycin-based therapy.
Matsumura
M
M
The Institute for Adult Diseases, Asahi Life Foundation, 1-9-14 Nishi-Shinjuku, Shinjuku-ku, Tokyo 160-0023, Japan. m-matsumura@asahi-life.or.jp
Hikiba
Y
Y
Ogura
K
K
Togo
G
G
Tsukuda
I
I
Ushikawa
K
K
Shiratori
Y
Y
Omata
M
M
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001483:Base Sequence; D017291:Clarithromycin / Q000494:pharmacology*; D004275:DNA, Ribosomal / Q000235:genetics* / Q000302:isolation & purification; D004352:Drug Resistance, Microbial; D005753:Gastric Mucosa / Q000382:microbiology; D016481:Helicobacter Infections / Q000175:diagnosis; D016480:Helicobacter pylori / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D009691:Nucleic Acid Denaturation; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D012338:RNA, Ribosomal, 23S / Q000235:genetics*
D000900:Anti-Bacterial Agents; D004275:DNA, Ribosomal; D012338:RNA, Ribosomal, 23S; D017291:Clarithromycin