[Genotyping cytochrome P450IIE1 in alcoholic liver diseases and its significance].
OBJECTIVE
To study the relationship between genotypes of cytochrome P450IIE1 (IIE1 ) and development of chronic liver diseases.


METHODS
Cytochrome P450IIE1 was genotyped by PCR and restricted endonucleases digestion of DNA prepared from peripheral white blood cells.


RESULTS
IIE1 could be divided into three types: A, B and C based on different products digested by two endonucleases. In normal controls predominant genotypes were A and B. No relationship was found between development of non-alcoholic chronic liver diseases and IIE1 genotypes. Its genotype distribution was similar to normal controls. Detection of type A in alcoholic liver diseases was significantly decreased and type B was significantly increased (P<0.01). Detection of type A in alcohol-related hepatocellular carcinoma was also decreased and type B increased compared to non-alcohol-related hepatocellular carcinoma.


CONCLUSIONS
IIE1 genotypes play some roles in development of alcoholic liver diseases and alcohol-related hepatocellular carcinoma.
Zhang
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S
Department of Gastroenterology, Zhongshan Hospital, Shanghai 200032, China.
Liu
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S
Liu
H
H
Zhu
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W
D000328:Adult; D006528:Carcinoma, Hepatocellular / Q000201:enzymology / Q000235:genetics; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008108:Liver Diseases, Alcoholic / Q000201:enzymology* / Q000235:genetics; D008113:Liver Neoplasms / Q000201:enzymology / Q000235:genetics; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic
D019392:Cytochrome P-450 CYP2E1