[The role of chemokines in nasal polyps].
Nasal polyposis is an inflammatory condition of the nose and the sinuses characterised by a marked infiltration of eosinophils in addition to lymphocytes, mast cells and macrophages. The selective recruitment of eosinophils to inflammatory sites is mediated by CC chemokines such as Eotaxin and Eotaxin-2. In the present study histology, immunohistochemistry and ELISA were performed. The levels of Eotaxin and Eotaxin-2 and for comparison other chemokines RANTES and IL-8 were measured in nasal polyp tissue and in control nasal tissue. On histological examination 6 polyps showed an oedematous structure, one was glandular and one had a fibromatous pattern, while all showed a marked eosinophil infiltration. Immunohistochemistry of the polyps showed that epithelial cells were strongly positive for Eotaxin and IL-8, whereas endothelial cells stained positive for Eotaxin-2. Significantly higher amounts of Eotaxin, Eotaxin-2 and IL-8 were detected in polyp tissue when compared with control middle turbinates. The increased levels of eosinophil-stimulating chemokines, such as Eotaxin and Eotaxin-2 in nasal polyps suggest that they may be important regulators of eosinophil recruitment in this inflammatory disease.
Caversaccio
M
M
Universitätsklinik für HNO, Hals- und Kopfchirurgie, Inselspital Bern.
Hartnell
A
A
Calnan
D
D
Jose
P
P
Mackay
I S
IS
Uguccioni
M
M
Baggiolini
M
M
Williams
T J
TJ
Conroy
D M
DM
D054413:Chemokine CCL11; D054423:Chemokine CCL24; D018946:Chemokine CCL5 / Q000032:analysis; D018925:Chemokines / Q000032:analysis* / Q000502:physiology; D019742:Chemokines, CC / Q000032:analysis; D016207:Cytokines / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D004804:Eosinophils / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D016906:Interleukin-9 / Q000032:analysis; D009297:Nasal Mucosa / Q000473:pathology; D009298:Nasal Polyps / Q000473:pathology* / Q000503:physiopathology*
C515456:CCL11 protein, human; C515484:CCL24 protein, human; D054413:Chemokine CCL11; D054423:Chemokine CCL24; D018946:Chemokine CCL5; D018925:Chemokines; D019742:Chemokines, CC; D016207:Cytokines; D016906:Interleukin-9