Phenotypic and functional characteristics of BM-derived DC from NOD and non-diabetes-prone strains.
We compared BM-derived DC from NOD with DC from non-diabetes-prone strains. NOD myeloid progenitors cultured in GM or GM + IL-4 yielded lower numbers of DC than similar cultures from other strains. NOD GM + IL-4 DC produced lower amounts of IL-12 p70 following CD40 ligation or LPS stimulation in the presence of IFN-gamma than DC from other strains, although similar levels of IL-6 and NO were produced by all strains. The low amounts of IL-12 p70 produced by GM + IL-4 DC are despite a more mature DC phenotype observed in NOD mice. The low DC number could diminish the ability of DC to stimulate immunoregulatory T cells. Furthermore, the differentiation/maturation of DC from myeloid progenitors is altered, reflecting disorders in the function of these cells in NOD BM.
Feili-Hariri
M
M
Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA.
Morel
P A
PA
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000378:metabolism* / Q000502:physiology*; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D018664:Interleukin-12 / Q000378:metabolism; D015847:Interleukin-4 / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D016688:Mice, Inbred NOD / Q000235:genetics*; D010641:Phenotype
D018664:Interleukin-12; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor