title: Impaired cerebral cortical gray matter growth after treatment with dexamethasone for neonatal chronic lung disease.
abstract: OBJECTIVE
  The specific aim of this study was to quantify at term the influence of postnatal systemic dexamethasone treatment for neonatal chronic lung disease on subsequent brain growth and development in premature infants without evidence of severe intraventricular hemorrhage or white matter injury.
  
  
  METHODS
  Eighteen premature (23 to 31 weeks) infants, 7 treated with dexamethasone and 11 not treated, were studied at term, ie, 38 to 41 postconceptional weeks, by an advanced quantitative volumetric 3-dimensional magnetic resonance imaging (MRI) technique to quantify cerebral tissue volumes. Fourteen healthy term infants also were studied for comparison. A sequence of image processing algorithms was used to segment each of the MRI slices into the following separate tissue classes: cerebral cortical gray matter, basal ganglia/thalami, unmyelinated white matter, myelinated white matter, and cerebrospinal fluid, all classified based on magnetic resonance signal intensity and anatomic location. A final summing of voxels for each tissue class was performed to compute absolute volumes in milliliters.
  
  
  RESULTS
  Cerebral cortical gray matter volume in premature infants treated with dexamethasone was reduced 35% when compared with gray matter volume in premature infants not treated with dexamethasone (mean +/- standard deviation, 130.3 +/- 54.0 vs 200.6 +/- 35.1 mL, respectively). Subcortical gray matter volumes (basal ganglia and thalami) and myelinated and unmyelinated white matter volumes were not significantly different among the treated and untreated groups. However, premature infants treated with dexamethasone exhibited a reduction (30%) in total cerebral tissue volume compared with total cerebral tissue volume in both the premature infants not treated with dexamethasone and the control term infants (312.7 +/- 43.7 vs 448.2 +/- 50.2 and 471.6 +/- 36.4 mL respectively). This latter finding relates primarily to the decrease in cerebral cortical gray matter volume.
  
  
  CONCLUSIONS
  The data suggest an impairment in brain growth, principally affecting cerebral cortical gray matter, secondary to systemic dexamethasone therapy. Although the premature infants who received dexamethasone were smaller with more severe respiratory disease, these findings are consistent with growing evidence of a potential deleterious effect of dexamethasone on neonatal brain and subsequent neurodevelopmental outcome. This apparent deleterious effect should be taken into consideration by clinicians when weighing the potential risks and benefits of this therapy for low birth weight infants with neonatal chronic lung disease.
authors.affiliation: Division of Newborn Medicine, Harvard Medical School and Brigham & Women's Hospital, Boston, Massachusetts, USA.
authors.forename: B P
authors.initials: BP
authors.lastname: Murphy
authors.forename: T E
authors.initials: TE
authors.lastname: Inder
authors.forename: P S
authors.initials: PS
authors.lastname: Huppi
authors.forename: S
authors.initials: S
authors.lastname: Warfield
authors.forename: G P
authors.initials: GP
authors.lastname: Zientara
authors.forename: R
authors.initials: R
authors.lastname: Kikinis
authors.forename: F A
authors.initials: FA
authors.lastname: Jolesz
authors.forename: J J
authors.initials: JJ
authors.lastname: Volpe
mesh_terms: D016022:Case-Control Studies; D002540:Cerebral Cortex / Q000033:anatomy & histology / Q000254:growth & development*; D002555:Cerebrospinal Fluid; D002908:Chronic Disease; D003907:Dexamethasone / Q000009:adverse effects / Q000627:therapeutic use*; D005938:Glucocorticoids / Q000009:adverse effects / Q000627:therapeutic use*; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature / Q000254:growth & development*; D007235:Infant, Premature, Diseases / Q000188:drug therapy* / Q000503:physiopathology; D019102:Infant, Very Low Birth Weight; D008171:Lung Diseases / Q000188:drug therapy* / Q000503:physiopathology; D008279:Magnetic Resonance Imaging; D015430:Weight Gain
chemical_list: D005938:Glucocorticoids; D003907:Dexamethasone