Polymorphisms of the renin gene promoter in spontaneously hypertensive and Wistar-Kyoto rats.
1. In the present study, 1.39 kb of the renin gene 5' region in spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats was amplified by polymerase chain reaction from genomic DNA and sequenced. Consistent differences in the renin gene sequence of SHR and WKY rats were found at positions -725, -727, -979 and -1126/-1129 as numbered from the transcription start site (+1). No polymorphism was specific to hypertensive rats. 2. Gel-shift assays were performed using labelled SHR renin promotor DNA and nuclear proteins extracted from rat kidneys. The regions between -1122 and -1139 and between -701 and -797 showed protein binding.
Leckie
B J
BJ
Department of Medicine and Therapeutics, Leicester Royal Infirmary, United Kingdom. bjl2@le.ac.uk
D000818:Animals; D001483:Base Sequence; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D051381:Rats; D011918:Rats, Inbred SHR / Q000235:genetics*; D011921:Rats, Inbred WKY / Q000235:genetics*; D012083:Renin / Q000235:genetics*
D012083:Renin