Restraint stress applied prior to chemical sensitization modulates the development of allergic contact dermatitis differently than restraint prior to challenge.
BALB/c mice were sensitized and challenged on the skin with the contact sensitizer, dinitroflourobenzene. Acute restraint applied before sensitization diminished, whereas restraint administered before challenge enhanced, chemical-induced ear swelling and leukocytic infiltration in the dermis. Administration of RU486, a glucocorticoid receptor antagonist, partially reversed restraint modulation of the ear swelling response in both restraint paradigms. Restraint did not modulate significantly the concentration of TNF-alpha and IL-1 beta in ear tissue homogenates. These data show that acute restraint modulates cutaneous sensitization differently than challenge, but the changes are not reflected in TNF-alpha or IL-l beta production.
Flint
M S
MS
Toxicology and Molecular Biology Branch, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV 26505, USA.
Valosen
J M
JM
Johnson
E A
EA
Miller
D B
DB
Tinkle
S S
SS
D000818:Animals; D003345:Corticosterone / Q000097:blood; D017449:Dermatitis, Allergic Contact / Q000276:immunology*; D004139:Dinitrofluorobenzene; D004431:Ear, External; D004487:Edema / Q000139:chemically induced / Q000276:immunology; D006727:Hormone Antagonists / Q000494:pharmacology; D007107:Immune System / Q000187:drug effects / Q000276:immunology; D007375:Interleukin-1 / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015735:Mifepristone / Q000494:pharmacology; D012149:Restraint, Physical; D012867:Skin / Q000276:immunology / Q000378:metabolism; D013312:Stress, Physiological / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology / Q000378:metabolism
D006727:Hormone Antagonists; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D015735:Mifepristone; D004139:Dinitrofluorobenzene; D003345:Corticosterone