Insulin deficiency and reduced expression of lipogenic enzymes in cardiomyopathic hamster.
Evidence is given that the heart of the cardiomyopathic UM-X7.1 hamster has a lipid composition different from that of the same tissue isolated from animals of the Syrian hamster parent strain. Also, noncardiac tissues from cardiomyopathic and healthy hamsters exhibit significant compositional differences. On the basis of these preliminary observations, a comparative study of the hepatic biosynthesis of lipids in cardiomyopathic and healthy Syrian hamsters was undertaken. The results obtained indicate that the cardiomyopathic hamster is characterized by a generalized disturbance of lipid metabolism. In particular, the fatty acid synthase and stearoyl-CoA desaturase activities were significantly lower in the liver of UM-X7.1 hamsters than in age-matched healthy controls fed the same diet. Northern blot analysis of the mRNAs encoding the two enzymatic proteins and the "lipogenic" S14 nuclear protein indicated that the transcription of the respective genes was impaired in UM-X7.1.Short-term dietary manipulations modulated the expression of the above-mentioned genes both in cardiomyopathic and healthy animals. However, dietary carbohydrates were less effective in inducing the expression of lipogenic enzymes in UM-X7.1 liver than healthy controls. The main determinant of the metabolic defect pointed out in the present work appears to be represented by the low insulin level detectable in the plasma of the cardiomyopathic hamster.-Vecchini, A., L. Binaglia, M. Bibeau, M. Minieri, F. Carotenuto, and P. Di Nardo. Insulin deficiency and reduced expression of lipogenic enzymes in cardiomyopathic hamster. J. Lipid Res. 2001. 42: 96;-105.
Vecchini
A
A
Institute of Biochemistry, Faculty of Medicine, University of Perugia, 06126 Perugia, Italy.
Binaglia
L
L
Bibeau
M
M
Minieri
M
M
Carotenuto
F
F
Di Nardo
P
P
D000367:Age Factors; D000818:Animals; D009202:Cardiomyopathies / Q000178:diet therapy / Q000201:enzymology* / Q000378:metabolism; D006224:Cricetinae; D018752:Diet, Fat-Restricted; D004040:Dietary Carbohydrates / Q000627:therapeutic use; D064429:Fatty Acid Synthases / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D006352:Heart Ventricles / Q000737:chemistry; D007328:Insulin / Q000097:blood / Q000172:deficiency*; D008099:Liver / Q000737:chemistry / Q000201:enzymology; D008647:Mesocricetus; D023421:Models, Animal; D009687:Nuclear Proteins; D010743:Phospholipids / Q000032:analysis / Q000096:biosynthesis* / Q000493:pharmacokinetics; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D011849:Radioactive Tracers; D013230:Stearoyl-CoA Desaturase / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors
D004040:Dietary Carbohydrates; D007328:Insulin; D009687:Nuclear Proteins; D010743:Phospholipids; D011506:Proteins; D012333:RNA, Messenger; D011849:Radioactive Tracers; C051065:THRSP protein, human; D014157:Transcription Factors; D013230:Stearoyl-CoA Desaturase; D064429:Fatty Acid Synthases