title: Aeromonas hydrophila and its relation with drinking water indicators of microbiological quality in Argentine.
abstract: In Argentine, water municipal supplies disinfection is carried out by chlorine. We have isolated Aeromonas hydrophila from a chlorinated water supply in Buenos Aires that fulfilled Argentinean microbiological quality standards. It is an aquatic organism that could produce cytotoxins and enterotoxins associated with acute gastroenteritis and wound infections in human and hemorrhagic septicaemia of fish, reptiles and amphibians. The isolated strain produced protein bands at isoelectric point in the range of 4.6-5.3 lightly labile at 56 degrees C and heat labile at 100 degrees C non cholera antitoxin reactive in electrofocusing assay (IEF) and showed hemolytic activity thermolabile at 56 degrees C and 100 degrees C in 5% rabbit erythrocytes in phosphate buffered saline. A cytotoxic effect thermolabile at 56 degrees C and 100 degrees C and a cytotonic activity were demonstrated in Vero cell cultures. Survival assay of A. hydrophila and indicator organisms would show no correlation between their contamination sources. Biofilms production could explain the persistence of this gram negative pathogen organism in chlorinated tap water. A water system supply maintenance program and pasteurisation may be performed in spite of water chlorination.
authors.affiliation: Instituto Nacional de Medicamentos, Buenos Aires, Argentina.
authors.forename: M C
authors.initials: MC
authors.lastname: Fernández
authors.forename: B N
authors.initials: BN
authors.lastname: Giampaolo
authors.forename: S B
authors.initials: SB
authors.lastname: Ibañez
authors.forename: M V
authors.initials: MV
authors.lastname: Guagliardo
authors.forename: M M
authors.initials: MM
authors.lastname: Esnaola
authors.forename: L
authors.initials: L
authors.lastname: Conca
authors.forename: P
authors.initials: P
authors.lastname: Valdivia
authors.forename: S M
authors.initials: SM
authors.lastname: Stagnaro
authors.forename: C
authors.initials: C
authors.lastname: Chiale
authors.forename: H
authors.initials: H
authors.lastname: Frade
mesh_terms: D016980:Aeromonas hydrophila / Q000737:chemistry / Q000187:drug effects / Q000302:isolation & purification* / Q000472:pathogenicity; D000818:Animals; D001118:Argentina; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development; D015153:Blotting, Western; D002713:Chlorine / Q000494:pharmacology; D002522:Chlorocebus aethiops; D003603:Cytotoxins / Q000302:isolation & purification; D006461:Hemolysis; D007525:Isoelectric Focusing; D014709:Vero Cells; D014774:Virulence; D014881:Water Supply / Q000032:analysis* / Q000592:standards*
chemical_list: D003603:Cytotoxins; D002713:Chlorine