[Physical location and detection of homologous sequences of proto-oncogene ras on maize].
Proto-oncogene ras is an important gene for suppress the animal cells apoptosis. To analyze the presence of homologous sequences of ras in maize and rice, Southern blot hybridization was performed and positive signals was detected in these two species' genomes. The physical location of the ras homologous sequences was also carried out in maize chromosome by FISH. ras was hybridized onto the long arm of the chromosomes 2 and 7. The detection rates of FISH were 10.85% and 14.15%, and percentage distances from centromere to detection sites were 54.92 +/- 1.90 and 94.62 +/- 2.77 respectively. These results provided important clues of the further research of plant apoptosis.
Yang
Z
Z
Center of Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
Cai
C L
CL
Qin
R
R
Liu
L H
LH
Song
Y C
YC
D017209:Apoptosis; D015139:Blotting, Southern; D011905:Genes, ras; D017404:In Situ Hybridization, Fluorescence; D012275:Oryza / Q000166:cytology / Q000235:genetics; D017385:Sequence Homology; D003313:Zea mays / Q000166:cytology / Q000235:genetics*