[Study of topical ketorolac effect on arachidonic acid metabolism in experimental anterior uveitis].
OBJECTIVE
To study the effect of topical ketorolac-tromethamine in the arachidonic acid metabolism on a model of endotoxin induced uveitis (EIU) in albino rabbits.


METHODS
EIU was produced by intravitreal injection in the right eye with 10 ng LPS A Salmonella typhimurium endotoxin in 5ul saline solution. We used 60 animals (5 groups of 12 animals each). Control group (G-I) was injected with saline (5 ul); endotoxin group (G-II) was injected with 10 ng of ET; group III, IV and V were injected with the same amount of ET and treated with topical ketorolac-tromethamine every 6, 4 and 2 hours respectively. The animals were sacrificed 24 hours after the ET administration and we determined E2 prostaglandin and B4 leukotriene concentration in the aqueous humor.


RESULTS
In all treated groups with ketorolac-tromethamine we observed a significant reduction (p<0.05) in aqueous humor concentration of E2 prostaglandin without increase of the B4 leukotriene concentration comparing with endotoxin group.


CONCLUSIONS
Ketorolac tromethamine produced an inhibition of ciclooxigenase metabolism of arachidonic acid without increase of lypoxygenase pathway.
Cuevas Andrés R
Servicio de Oftalmología, Hospital Miguel Servet, Zaragoza, España.
Ruiz Moreno O
Ferrer Novella E
Torrón Fernández-Blanco C
Rojo Aragües A
Pablo Júlvez LE
Honrubia López FM
D000287:Administration, Topical; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage*; D016718:Arachidonic Acid / Q000378:metabolism*; D020910:Ketorolac / Q000008:administration & dosage*; D011817:Rabbits; D014605:Uveitis / Q000378:metabolism*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D016718:Arachidonic Acid; D020910:Ketorolac