title: Direct proof for the presence and expression of two 5% different 16S rRNA genes in individual cells of Haloarcula marismortui.
abstract: A single-cell-derived pure culture of Haloarcula marismortui was obtained using the optical tweezers technique. Analysis of the 16S rRNA genes unambiguously demonstrated two 5% different 16S rRNA sequences. In addition, expression of both operons in the same cell was shown by fluorescence in situ hybridization with operon-specific probes.
authors.affiliation: Lehrstuhl für Mikrobiologie, Universität Regensburg, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Amann
authors.forename: K O
authors.initials: KO
authors.lastname: Stetter
authors.forename: E
authors.initials: E
authors.lastname: Llobet-Brossa
authors.forename: R
authors.initials: R
authors.lastname: Amann
authors.forename: J
authors.initials: J
authors.lastname: Antón
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D019614:Haloarcula marismortui / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*
chemical_list: D017931:DNA Primers; D012336:RNA, Ribosomal, 16S