title: Characterization of the 16S rRNA gene V2 region and the rrn operons of Gardnerella vaginalis.
abstract: Ribosomal RNA (rRNA) gene polymorphism was apparent when Gardnerella vaginalis DNA restriction profiles were hybridized with nonradioactively labeled total rRNA isolated from this bacterium. In contrast, use of a polymerase chain reaction (PCR)-based 16S rRNA gene V2 region resulted in a 118-bp V2-PCR amplicon that was specific and common in all 30 tested G. vaginalis isolates. In addition to providing a G. vaginalis-specific fingerprint, when the V2-PCR amplicon along with total rRNA were utilized as probes, a partial rRNA gene restriction map could be constructed. G. vaginalis contains two rrn operons with an EcoRI fragment of 1.6 kb common to both.
authors.affiliation: Long Island University, Department of Biology, Brookville, NY 11548-1300, USA. knath@liu.edu
authors.forename: K
authors.initials: K
authors.lastname: Nath
authors.forename: X
authors.initials: X
authors.lastname: Chen
authors.forename: K S
authors.initials: KS
authors.lastname: Ahn
authors.forename: S
authors.initials: S
authors.lastname: Chen
mesh_terms: D016172:DNA Fingerprinting; D015342:DNA Probes / Q000235:genetics; D006194:Gardnerella vaginalis / Q000235:genetics*; D018628:Gene Dosage; D020459:Genes, rRNA / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000737:chemistry / Q000235:genetics; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D015183:Restriction Mapping; D012407:rRNA Operon
chemical_list: D015342:DNA Probes; D007135:Immunoglobulin Variable Region; D012336:RNA, Ribosomal, 16S