title: Two-step nature of human T-cell leukemia virus type 1 replication in experimentally infected squirrel monkeys (Saimiri sciureus).
abstract: After experimental infection of squirrel monkeys (Saimiri sciureus) with human T-cell leukemia virus type 1 (HTLV-1)-infected cells, the virus is transcribed only transiently in circulating blood, spleen, and lymph nodes. Stable disappearance of viral expression occurs at 2 to 3 weeks after inoculation. This coincides with the development of the anti-HTLV-1 immune response and persistent detection of the provirus in peripheral blood mononuclear cells (PBMCs). In this study, the HTLV-1 replication pattern was analyzed over time in PBMCs and various organs from two HTLV-1-infected squirrel monkeys. Real-time quantitative PCR confirmed that PBMCs and lymphoid organs constitute the major reservoirs for HTLV-1. The PCR amplification of HTLV-1 flanking sequences from PBMCs evidenced a pattern of clonal expansion of infected cells identical to that observed in humans. Dissemination of the virus in body compartments appeared to result from cellular transport of the integrated provirus. The circulating proviral burden increased as a function of time in one animal studied over a period of 4 years. The high proviral loads observed in the last samples resulted from the accumulation of infected cells via the extensive proliferation of a restricted number of persistent clones on a background of polyclonally expanded HTLV-1-positive cells. Therefore, HTLV-1 primary infection in squirrel monkeys is a two-step process involving a transient phase of reverse transcription followed by persistent multiplication of infected cells. This suggests that the choice of the target for blocking HTLV-1 replication might depend on the stage of infection.
authors.affiliation: Unité 524 INSERM, Institut de Recherche sur le Cancer de Lille, Lille, France.
authors.forename: F
authors.initials: F
authors.lastname: Mortreux
authors.forename: M
authors.initials: M
authors.lastname: Kazanji
authors.forename: A S
authors.initials: AS
authors.lastname: Gabet
authors.forename: B
authors.initials: B
authors.lastname: de Thoisy
authors.forename: E
authors.initials: E
authors.lastname: Wattel
mesh_terms: D000818:Animals; D002460:Cell Line; D002999:Clone Cells; D004279:DNA, Viral / Q000032:analysis; D015490:HTLV-I Infections / Q000503:physiopathology / Q000821:virology*; D015368:Human T-lymphotropic virus 1 / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology; D008213:Lymphocyte Activation; D008221:Lymphoid Tissue / Q000821:virology; D008297:Male; D011533:Proviruses / Q000235:genetics; D012453:Saimiri; D019562:Viral Load; D014779:Virus Replication
chemical_list: D004279:DNA, Viral