Regulation of benzodiazepine receptor binding and GABA(A) subunit mRNA expression by punishment and acute alprazolam administration.
Quantitative autoradiography of benzodiazepine (BZ) receptors and competitive reverse transcription-polymerase chain reaction were used to characterize changes in BZ binding and GABA(A) receptor subunit transcription levels associated with the anxiolytic effects of alprazolam. Effects were assessed on punished and non-suppressed water consumption using a lick suppression (Vogel) paradigm. Alprazolam had no effect on non-suppressed licking, [(3)H]Ro 15-1788 binding or receptor subunit transcript levels, compared to non-drug controls. When each fifth lick produced a shock (0-0.5 mA), responding was suppressed in an intensity-related manner. The highest intensity significantly decreased licking (85%), [(3)H]Ro 15-1788 binding (12%) and alpha1 transcript levels (63%) in the basolateral nucleus of the amygdala, and [(3)H]Ro 15-1788 binding in the mediodorsal thalamic nucleus (15%), compared to non-punished controls. Punishment increased the ratio of gamma2L/S transcripts in the basolateral nucleus of the amygdala. Alprazolam blocked or reversed each of these effects. These results show that punishment has similar effects on BZ binding and GABA(A) receptor subunit expression and that alprazolam can block or reverse those effects. Such changes may be related to the anxiolytic effects of alprazolam.
Liu
M
M
Department of Pharmacology and Therapeutics, Louisiana State University Health Sciences Center in Shreveport, 1501 Kings Highway, P.O. Box 33932, Shreveport, LA 71130-3932, USA.
Glowa
J R
JR
D000525:Alprazolam / Q000494:pharmacology; D000679:Amygdala / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D018757:GABA Modulators / Q000494:pharmacology; D008297:Male; D011678:Punishment; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000187:drug effects / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018757:GABA Modulators; D012333:RNA, Messenger; D011963:Receptors, GABA-A; D000525:Alprazolam