title: Role of phospholipase D-derived phosphatidic acid as a substrate for phospholipase A2 in RBL-2H3 cells.
abstract: The implications of phospholipase D (PLD) in cytosolic phospholipase A2 (cPLA2) activation were studied in a mast cell line, RBL-2H3, upon stimulation with antigen. Antigen-stimulated prostaglandin D2 generation was apparently suppressed by ethanol with a concomitant decrease in phosphatidic acid (PA) formation. The prostaglandin D2 generation was also inhibited almost completely by methyl arachidonyl fluorophosphonate (MAFP), an inhibitor of cPLA2, but not by diacylglycerol lipase inhibitor. Furthermore, stimulation with antigen resulted in an increase in lysophosphatidic acid formation, which was suppressed by MAFP in parallel with an increase in PA formation. These results suggest that PA formed by the catalytic action of PLD is used as a substrate for cPLA2, thus PLD regulates cPLA2 activation in antigen-stimulated RBL-2H3 cells.
authors.affiliation: Department of Pathological Biochemistry, Kyoto Pharmaceutical University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kitatani
authors.forename: S
authors.initials: S
authors.lastname: Akiba
authors.forename: T
authors.initials: T
authors.lastname: Sato
mesh_terms: D000818:Animals; D000941:Antigens / Q000494:pharmacology; D001095:Arachidonic Acids / Q000494:pharmacology; D003600:Cytosol / Q000201:enzymology; D004140:Dinitrophenols / Q000494:pharmacology; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015471:Leukemia, Basophilic, Acute / Q000201:enzymology / Q000378:metabolism; D063065:Organophosphonates; D010712:Phosphatidic Acids / Q000378:metabolism*; D010739:Phospholipase D / Q000378:metabolism*; D010741:Phospholipases A / Q000037:antagonists & inhibitors / Q000378:metabolism*; D054467:Phospholipases A2; D015230:Prostaglandin D2 / Q000096:biosynthesis; D051381:Rats; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D000941:Antigens; D001095:Arachidonic Acids; D004140:Dinitrophenols; D004791:Enzyme Inhibitors; D063065:Organophosphonates; D010712:Phosphatidic Acids; C034638:dinitrophenyl-bovine serum albumin; C104810:methyl arachidonylfluorophosphonate; D012710:Serum Albumin, Bovine; D010741:Phospholipases A; D054467:Phospholipases A2; D010739:Phospholipase D; D015230:Prostaglandin D2