title: Genomic exploration of the hemiascomycetous yeasts: 7. Saccharomyces servazzii.
abstract: The genome of Saccharomyces servazzii was analyzed with 2570 random sequence tags totalling 2.3 Mb. BLASTX comparisons revealed a minimum of 1420 putative open reading frames with significant homology to Saccharomyces cerevisiae (58% aa identity on average), two with Schizosaccharomyces pombe and one with a human protein, confirming that S. servazzii is closely related to S. cerevisiae. About 25% of the S. servazzii genes were identified, assuming that the gene complement is identical in both yeasts. S. servazzii carries very few transposable elements related to Ty elements in S. cerevisiae. Most of the mitochondrial genes were identified in eight contigs altogether spanning 25 kb for a predicted size of 29 kb. A significant match with the Kluyveromyces lactis linear DNA plasmid pGKL-1 encoded RF4 killer protein suggests that a related plasmid exists in S. servazzii. The sequences have been deposited with EMBL under the accession numbers AL402279-AL404848.
authors.affiliation: Collection de Levures d'Intérêt Biotechnologie, Laboratoire de Génétique Moléculaire et Cellulaire, INRA UMR216, CNRS URA1925, INA-PG, Thiverval-Grignon, France. serge@grignon.inra.fr
authors.forename: S
authors.initials: S
authors.lastname: Casaregola
authors.forename: A
authors.initials: A
authors.lastname: Lépingle
authors.forename: E
authors.initials: E
authors.lastname: Bon
authors.forename: C
authors.initials: C
authors.lastname: Neuvéglise
authors.forename: H
authors.initials: H
authors.lastname: Nguyen
authors.forename: F
authors.initials: F
authors.lastname: Artiguenave
authors.forename: P
authors.initials: P
authors.lastname: Wincker
authors.forename: C
authors.initials: C
authors.lastname: Gaillardin
mesh_terms: D001203:Ascomycota / Q000235:genetics; D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D005656:Fungal Proteins / Q000145:classification / Q000235:genetics; D020440:Gene Duplication; D016681:Genome, Fungal; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D018626:Retroelements; D012440:Saccharomyces / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D017381:Spliceosomes / Q000235:genetics
chemical_list: D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D005656:Fungal Proteins; D009687:Nuclear Proteins; D018626:Retroelements