title: Tissue culture of the deep-sea bivalve Calyptogena soyoae.
abstract: Tissue culture for the deep-sea clam Calyptogena soyoae (C. soyoae) has been examined. Mantle tissue was cultured in Dulbecco's modified Eagle medium that was prepared using artificial seawater supplemented with fetal bovine serum (FBS) and the body fluid of C. soyoae. The mantle cells were viable in culture for at least 13 days at 4 degrees C and atmospheric pressure on a polylysine-coated dish, although no cells attached in the body fluid-free culture medium. It was found that mantle cells synthesized DNA and seemed to proliferate under atmospheric conditions.
authors.affiliation: Frontier Research Program for Deep-sea Extremophiles, Japan Marine Science and Technology Center, Yokosuka. skoyama@jamstec.go.jp
authors.forename: S
authors.initials: S
authors.lastname: Koyama
authors.forename: M
authors.initials: M
authors.lastname: Aizawa
mesh_terms: D000818:Animals; D002455:Cell Division; D046508:Culture Techniques; D004261:DNA Replication; D008974:Mollusca / Q000166:cytology* / Q000378:metabolism