Lack of replicative senescence in normal rodent glia.
Replicative senescence is thought to be an intrinsic mechanism for limiting the proliferative life-span of normal somatic cells. We show here that rat Schwann cells can be expanded indefinitely in culture while maintaining checkpoints normally lost during the immortalization process. These findings demonstrate that senescence is not an inevitable consequence of extended proliferation in culture.
Mathon
N F
NF
MRC Laboratory for Molecular Cell Biology, University College London, Gower Street, London WC1E 6BT, UK.
Malcolm
D S
DS
Harrisingh
M C
MC
Cheng
L
L
Lloyd
A C
AC
D000818:Animals; D001769:Blood; D002352:Carrier Proteins / Q000378:metabolism; D018929:Cell Culture Techniques; D002455:Cell Division; D002460:Cell Line; D048429:Cell Size; D002478:Cells, Cultured; D016922:Cellular Senescence; D002999:Clone Cells; D003470:Culture Media; D019941:Cyclin-Dependent Kinase Inhibitor p16; D018844:Cyclin-Dependent Kinases / Q000378:metabolism; D016213:Cyclins / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D015726:Giant Cells / Q000166:cytology; D009154:Mutation; D010641:Phenotype; D011506:Proteins / Q000378:metabolism; D051381:Rats; D012583:Schwann Cells / Q000166:cytology* / Q000502:physiology; D019098:Telomerase / Q000378:metabolism; D016615:Telomere / Q000502:physiology; D025803:Tumor Suppressor Protein p14ARF; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism; D018631:ras Proteins / Q000378:metabolism
D002352:Carrier Proteins; D003470:Culture Media; D019941:Cyclin-Dependent Kinase Inhibitor p16; D016213:Cyclins; D011506:Proteins; D025803:Tumor Suppressor Protein p14ARF; D016159:Tumor Suppressor Protein p53; D018844:Cyclin-Dependent Kinases; D019098:Telomerase; D001616:beta-Galactosidase; D018631:ras Proteins