Recombinant expression analysis of natural and synthetic bovine alpha-casein in Escherichia coli.
As a prelude to developing a yeast-based fermentation process for the production of phenylalanine-free alpha-casein as a foodstuff for patients suffering from phenylketonuria, we cloned the gene encoding bovine alpha-casein. We synthesised a modified gene sequence encoding the same, but devoid of phenylalanine codons and with a codon bias similar to that of naturally occurring highly expressed genes in Saccharomyces cerevisiae. The results show that both gene sequences are readily expressed in Escherichia coli when cloned in an E. coli bacteriophage T7 promoter-driven plasmid vector. In this host, the natural and synthetic casein proteins were produced at levels equating to 18.0% and 7.6% of the cell's soluble protein, respectively.
Goda
S K
SK
CAMR, Salisbury, Wiltshire, UK.
Sharman
A F
AF
Yates
M
M
Mann
N
N
Carr
N
N
Minton
N P
NP
Brehm
J K
JK
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002364:Caseins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D002417:Cattle; D003001:Cloning, Molecular; D003062:Codon; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D005813:Genes, Synthetic; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010649:Phenylalanine / Q000235:genetics; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D012995:Solubility
D002364:Caseins; D003062:Codon; D011994:Recombinant Proteins; D010649:Phenylalanine