The correlation between immune rejection and osteoinduction of allogeneic bone grafting.
OBJECTIVE
To evaluate the relationship between the immune rejection and the osteoinductive potential of bone allograft.


METHODS
Allogeneic and syngeneic fresh bone, autolyzed antigen-extracted bone, bone matrix gelatin and demineralized bone matrix were implanted into the muscle of mice, and immunological tests, histological observation and alkaline phosphatase assay were performed.


RESULTS
Three and 6 weeks after implantation, all kinds of allogeneic implants activated immune rejection, among them, fresh bone induced the most vigorous immune rejection and bone matrix gelatin caused the weakest response. Allogeneic autolyzed antigen-extracted bone, bone matrix gelatin and demineralized bone matrix inhibited proliferation of the lymphocytes in vitro and bone matrix gelatin had the most powerful inhibiting effect. Both allogeneic and syngeneic autolyzed antigen-extracted bone, bone matrix gelatin, and demineralized bone matrix induced heterotopic osteogenesis in vivo and bone matrix gelatin had the best osteoinductive capacity.


CONCLUSIONS
There is a negative correlation between immune rejection to bone allograft and osteoinductive capacity of the graft.
Sun
L
L
Orthopaedic Center of CPLA, 88th Hospital, Tai'an 271000, China.
Hu
Y
Y
Ning
Z
Z
Liang
Z
Z
D000818:Animals; D018775:Bone Demineralization Technique; D001857:Bone Matrix; D016025:Bone Transplantation / Q000379:methods; D005780:Gelatin; D006084:Graft Rejection / Q000276:immunology* / Q000517:prevention & control; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018482:Muscle, Skeletal / Q000601:surgery; D014184:Transplantation, Homologous
D005780:Gelatin