title: Quantification of selected pesticide metabolites in human urine using isotope dilution high-performance liquid chromatography/tandem mass spectrometry.
abstract: The annual domestic use of pesticides is continually increasing, virtually ensuring that everyone is exposed to some level of pesticides on a regular basis through diet or environment. The potential developmental and physical adverse effects these chronic pesticide exposures have on children are of increasing concern. To adequately evaluate the potential adverse effects resulting from these exposures, accurate methods to measure the amount of the pesticide absorbed by the body must be developed. We have developed a sensitive method to measure the urinary metabolites of atrazine, diazinon, malathion, 2,4-dichlorophenoxyacetic acid (2,4-D), and certain synthetic pyrethroids in human urine. In our method, stable isotopically labeled analogues of the metabolites were spiked into the urine, which was subsequently extracted at both a neutral and acidic pH using organic solvents. The extracts were analyzed by high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) using atmospheric pressure chemical ionization. Our method has limits of detection ranging from 20 to 500 ng/l (parts per trillion) and relative standard deviations of less than 11%. This method has been used to measure the internal doses of these pesticides in both adults and children (n = 130) with no documented exposure to the pesticides. We detected atrazine and synthetic pyrethroid metabolites in less than 12% of the samples analyzed. The metabolites of 2,4-D, malathion, and diazinon were detected in 22%, 32%, and 57% of the samples, respectively.
authors.affiliation: Centers for Disease Control and Prevention, National Center for Environmental Health, Atlanta, Georgia 30341, USA.
authors.forename: S E
authors.initials: SE
authors.lastname: Baker
authors.forename: D B
authors.initials: DB
authors.lastname: Barr
authors.forename: W J
authors.initials: WJ
authors.lastname: Driskell
authors.forename: M D
authors.initials: MD
authors.lastname: Beeson
authors.forename: L L
authors.initials: LL
authors.lastname: Needham
mesh_terms: D000328:Adult; D002648:Child; D002851:Chromatography, High Pressure Liquid; D004781:Environmental Exposure / Q000032:analysis*; D004784:Environmental Monitoring / Q000379:methods; D005260:Female; D006801:Humans; D007553:Isotope Labeling; D008297:Male; D013058:Mass Spectrometry; D010573:Pesticide Residues / Q000652:urine*; D012680:Sensitivity and Specificity
chemical_list: D010573:Pesticide Residues