title: Analysis of interaction between molecules of Bombyx mori nucleopolyhedrovirus IE-2 using a yeast two-hybrid system.
abstract: Baculovirus IE-2 protein is one of well-known transactivators. In this report, we demonstrate that Bombyx mori nucleopolyhedrovirus (BmNPV) IE-2 interacts with itself. Several clones were obtained from a yeast two-hybrid screening system using IE-2 as bait and were found to encode IE-2 protein. Nucleotide sequencing of these clones showed that they contained C-terminal regions in common. Further analyses suggest that BmNPV IE-2 protein interacts with itself through 80 amino acid residues of coiled-coil domain in C-terminus.
authors.affiliation: Laboratory of Molecular Entomology and Baculovirology, RIKEN, 2-1 Hirosawa, Wako 351-0198, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Imai
authors.forename: W
authors.initials: W
authors.lastname: Kang
authors.forename: K
authors.initials: K
authors.lastname: Iwabuchi
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: S
authors.initials: S
authors.lastname: Maeda
mesh_terms: D000818:Animals; D012831:Bombyx / Q000821:virology*; D003001:Cloning, Molecular; D005821:Genetic Techniques; D017874:Immediate-Early Proteins / Q000235:genetics / Q000378:metabolism*; D017924:Nucleopolyhedroviruses / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation; D015003:Yeasts / Q000235:genetics
chemical_list: D017874:Immediate-Early Proteins; D015534:Trans-Activators; C072773:IE2 protein, polyhedrosis virus