title: Functional expression of IL-9 receptor by human neutrophils from asthmatic donors: role in IL-8 release.
abstract: Human polymorphonuclear neutrophils (PMNs) express surface receptors for various inflammatory mediators, including IgE and IL-4. Recently, the IL-9R locus has been genetically linked to asthma and bronchial hyperresponsiveness in humans. In this study, we evaluated expression of the IL-9R and the effect of IL-9 on human PMNs. RT-PCR analysis showed the presence of IL-9Ralpha-chain mRNA in PMN RNA preparations from asthmatic patients. Using FACS analysis, surface expression of IL-9Ralpha was detected on PMNs freshly isolated from asthmatics, and to a lesser extent on normal controls. In addition, protein expression of IL-9Ralpha was also detected in peripheral blood and bronchoalveolar lavage PMNs. Furthermore, functional studies showed that IL-9 stimulation of PMNs results in the release of IL-8 in a concentration-dependent manner. The anti-IL-9 neutralizing Ab suppressed this effect, but had no effect on GM-CSF-induced IL-8 release from PMNs. Taken together, these findings suggest a novel role for PMNs in allergic disease through the expression and activation of the IL-9R.
authors.affiliation: Meakins-Christie Laboratories, McGill University, Montreal, Quebec, Canada.
authors.forename: S
authors.initials: S
authors.lastname: Abdelilah
authors.forename: K
authors.initials: K
authors.lastname: Latifa
authors.forename: N
authors.initials: N
authors.lastname: Esra
authors.forename: L
authors.initials: L
authors.lastname: Cameron
authors.forename: L
authors.initials: L
authors.lastname: Bouchaib
authors.forename: N
authors.initials: N
authors.lastname: Nicolaides
authors.forename: R
authors.initials: R
authors.lastname: Levitt
authors.forename: Q
authors.initials: Q
authors.lastname: Hamid
mesh_terms: D001249:Asthma / Q000097:blood / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000276:immunology; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism; D018922:HL-60 Cells; D006801:Humans; D007150:Immunohistochemistry; D016209:Interleukin-8 / Q000378:metabolism*; D016906:Interleukin-9 / Q000378:metabolism* / Q000502:physiology; D009504:Neutrophils / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D018123:Receptors, Interleukin / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics / Q000502:physiology; D053651:Receptors, Interleukin-9
chemical_list: C508045:IL9R protein, human; D016209:Interleukin-8; D016906:Interleukin-9; D012333:RNA, Messenger; D018123:Receptors, Interleukin; D053651:Receptors, Interleukin-9