A novel mitochondrial septin-like protein, ARTS, mediates apoptosis dependent on its P-loop motif.
Here we describe a protein product of the human septin H5/PNUTL2/CDCrel2b gene, which we call ARTS (for apoptosis-related protein in the TGF-beta signalling pathway). ARTS is expressed in many cells and acts to enhance cell death induced by TGF-beta or, to a lesser extent, by other apoptotic agents. Unlike related septin gene products, ARTS is localized to mitochondria and translocates to the nucleus when apoptosis occurs. Mutation of the P-loop of ARTS abrogates its competence to activate caspase 3 and to induce apoptosis. Taken together, these observations expand the functional attributes of septins previously described as having roles in cytokinesis and cellular morphogenesis.
Larisch
S
S
Pathology Department, Rambam Medical Center, Haifa, Israel.
Yi
Y
Y
Lotan
R
R
Kerner
H
H
Eimerl
S
S
Tony Parks
W
W
Gottfried
Y
Y
Birkey Reffey
S
S
de Caestecker
M P
MP
Danielpour
D
D
Book-Melamed
N
N
Timberg
R
R
Duckett
C S
CS
Lechleider
R J
RJ
Steller
H
H
Orly
J
J
Kim
S J
SJ
Roberts
A B
AB
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001483:Base Sequence; D019556:COS Cells; D020169:Caspases / Q000378:metabolism; D003001:Cloning, Molecular; D003598:Cytoskeletal Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D017931:DNA Primers / Q000235:genetics; D004789:Enzyme Activation; D020558:GTP Phosphohydrolases / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D015870:Gene Expression; D006801:Humans; D008928:Mitochondria / Q000378:metabolism; D008969:Molecular Sequence Data; D058112:Septins; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000502:physiology
D003598:Cytoskeletal Proteins; D017931:DNA Primers; D016212:Transforming Growth Factor beta; D020169:Caspases; D020558:GTP Phosphohydrolases; C492027:SEPTIN4 protein, human; D058112:Septins