The effect of polymer coating biomaterials individually and incombination on the behavior of transformed RAW macrophage cells.
The use of homopolymers as coatings for biomaterials has received much attention in the last decade. However, the modifications and alterations induced by using such materials towards inflammatory cells have not been fully investigated. The specific objectives of this study were to investigate the role of hetero and homopolymers of amino acids on cell proliferation, and to determine the biochemical behavior associated with the incubation of RAW cells with various polymers. The RAW macrophage cell line was obtained from the American Type Culture Collection (Rockville, MD) and maintained in sterile media (RPMI) supplemented with 10% fetal bovine serum and 1% antibiotics and antimycotics. The cells were plated at a density of 1 x 10(6) cells/ml onto 24 well plates. The plates were divided into four groups of six wells per group per phase (24, 48, and 72 hours). Cells in the first group were treated with RGD, cells in group II were treated with poly-L-lysine, group III cells were treated with RGD + poly-L-lysine, and cells in group IV were treated with media alone, and served as controls. Cell number, as well as, protein, MDA, lactate dehydrogenase (LDH), and cytochrome C (cyto C) were determined at the end of 24, 48 and 72 hours. Data obtained from this investigation revealed that: (I) there were no significant difference in total cell counts between all experimental groups and control at the end of the 48 hour phase. However, at 24 hours there were fewer cells in the poly-L-lysine treated wells in comparison to control group (p < 0.05), (II) RGD and poly-L-lysine treatments did not cause changes in MDA or protein concentrations for the entire duration of the experiment, and (III) RGD treatment for 48 and 72 hours did not cause a reduction in the LDH activity compared to control and poly-L-lysine treated groups. Data obtained from this investigation could provide more insight regarding the design and development for safe and biocompatible orthopedic, dental and drug delivery devices.
Newman
D
D
University of Mississippi Medical Center, Jackson, MS 39216, USA.
Tucci
M
M
Puckett
A
A
Hughes
J
J
Benghuzzi
H
H
D001672:Biocompatible Materials / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002461:Cell Line, Transformed; D020099:Coated Materials, Biocompatible; D003574:Cytochrome c Group / Q000378:metabolism; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D009842:Oligopeptides / Q000494:pharmacology; D011107:Polylysine / Q000494:pharmacology; D011108:Polymers / Q000494:pharmacology*; D011506:Proteins / Q000378:metabolism
D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D003574:Cytochrome c Group; D009842:Oligopeptides; D011108:Polymers; D011506:Proteins; D011107:Polylysine; D008315:Malondialdehyde; C047981:arginyl-glycyl-aspartic acid; D007770:L-Lactate Dehydrogenase