HLA class I polymorphism, as characterised by PCR-SSOP, in a Brazilian exogamic population.
HLA-A, -B and -C genes were analysed in the population living in the metropolitan region of Curitiba, the main city of Parana State, southern Brazil, to provide data for studies and applications in HLA-related fields, and to contribute to the understanding of human microevolution. Heterozygosity is high (95-99%) for all three loci. Frequencies for most alleles and haplotypes of sub-Saharan African and of European ancestry presented a clear gradient between the White, Mulatto and Black subpopulations. Among Whites, the four most common haplotypes were A*01-Cw*07-B*0801, A*02-Cw*07-B*07, A*11-Cw*0401-B*35 and A*03-Cw*0401-B*35. Their frequencies ranged from 5.6% to 3.0%. In the Mulatto sub-population, six haplotypes presented very similar frequencies, close to 2.0-2.4%: A*02-Cw*03-B*15, A*02-Cw*0401-B*35, A*02-Cw*07-B*07, A*03-Cw*0401-B*35, A*30-Cw*17-B*4201, A*68-Cw*03-B*15. Haplotype A*30-Cw*17-B*4201 was found to be very common (6.6%) in the Black sub-population. Admixture estimate revealed the relative contributions of Europeans, sub-Saharan Africans and Amerindians to this populations which were, respectively, 94%, 3% and 3% for the White sub-population, 57%, 39% and 4% for the Mulatto sub-population, and 25%, 74% and 1% for the Black sub-population.
Braun-Prado
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Laboratory of Human Molecular Genetics, Federal University of Paraná, Curitiba, Brazil.
Vieira Mion
A L
AL
Farah Pereira
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N
Culpi
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Petzl-Erler
M L
ML
D000483:Alleles; D001938:Brazil; D005006:Ethnicity; D005787:Gene Frequency; D006680:HLA Antigens / Q000145:classification / Q000235:genetics*; D015234:HLA-A Antigens / Q000145:classification / Q000235:genetics; D015235:HLA-B Antigens / Q000145:classification / Q000235:genetics; D015236:HLA-C Antigens / Q000145:classification / Q000235:genetics; D006579:Heterozygote; D006650:Histocompatibility Testing; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic
D006680:HLA Antigens; D015234:HLA-A Antigens; D015235:HLA-B Antigens; D015236:HLA-C Antigens