Effect of rabbit kappa-casein expression on the properties of milk from transgenic mice.
Transgenic mice were produced carrying the coding region of the rabbit kappa-casein gene linked to the upstream region of the rabbit whey acidic protein gene. Mice from the highest-expressing line produced 2.5 mg rabbit kappa-casein/ml in their milk. The foreign protein was associated with the casein micelles and altered micelle size, though in the high-expressing line rabbit kappa-casein also segregated into the whey fraction obtained after centrifuging the milk samples. Milk from transgenic mice had the same overall protein content as that from non-transgenic mice, except for the transgene product. However, litters fed with this transgenic mouse milk grew less well than litters given milk from non-transgenic mice. This reduction in growth was not related to changes in mammary gland structure or mammary cell morphology. Preliminary results indicated that milk from the transgenic mice had a higher viscosity.
Hiripi
L
L
Agricultural Biotechnology Center, Gödöllo, Hungary.
Baranyi
M
M
Szabó
L
L
Tóth
S
S
Fontaine
M L
ML
Devinoy
E
E
Bösze
Z
Z
D000818:Animals; D015152:Blotting, Northern; D002364:Caseins / Q000235:genetics / Q000378:metabolism*; D012085:Chymosin / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D007774:Lactation; D008321:Mammary Glands, Animal / Q000378:metabolism / Q000648:ultrastructure; D051379:Mice; D008822:Mice, Transgenic; D008823:Micelles; D008854:Microscopy, Electron; D008892:Milk / Q000737:chemistry*; D008894:Milk Proteins / Q000235:genetics*; D011817:Rabbits; D013045:Species Specificity; D014783:Viscosity
D002364:Caseins; D008823:Micelles; D008894:Milk Proteins; C024471:whey acidic proteins; D012085:Chymosin