Immunofluorescence method for detection of Ebola virus immunoglobulin g, using HeLa cells which express recombinant nucleoprotein.
A novel recombinant baculovirus which expresses Ebola virus (EBO) nucleoprotein (NP) under the control of the cytomegalovirus immediate-early promoter was constructed. HeLa cells abortively infected with the baculovirus expressed EBO NP, and this was used as an immunofluorescent (IF) antigen to detect EBO immunoglobulin G (IgG) antibody. This IF method has high efficacy in detecting EBO IgG antibody in clinical specimens, indicating its usefulness in the diagnosis of EBO infections and seroepidemiological studies.
Saijo
M
M
Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Gakuen 4-7-1, Musahimurayama, Tokyo 208-0011, Japan.
Niikura
M
M
Morikawa
S
S
Kurane
I
I
D000914:Antibodies, Viral / Q000097:blood*; D016367:Baculoviridae; D003587:Cytomegalovirus / Q000235:genetics; D029043:Ebolavirus / Q000235:genetics / Q000276:immunology*; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods; D006367:HeLa Cells; D019142:Hemorrhagic Fever, Ebola / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis* / Q000097:blood*; D009698:Nucleoproteins / Q000276:immunology; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000276:immunology; D014162:Transfection; D014758:Viral Core Proteins / Q000276:immunology
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D009698:Nucleoproteins; D011994:Recombinant Proteins; D014758:Viral Core Proteins