title: In planta expression of a protein encoded by the extrachromosomal DNA of a phytoplasma and related to geminivirus replication proteins.
abstract: A new extrachromosomal DNA, EcOYW1, was cloned from the onion yellows phytoplasma (OY-W). Southern blot and PCR analysis showed that EcOYW1 is not present in the OY-M, a mild symptom line derived from OY-W. We determined the complete nucleotide sequence of EcOYW1; it is a circular dsDNA of 7.0 kbp in length, which contains seven ORFs. ORF1 encoded a homologue of the geminivirus Rep protein. Western immunoblot analysis revealed that this Rep homologue is expressed in OY-W infected plants, suggesting that EcOYW1 replicates via a geminivirus-like rolling-circle replication mechanism. EcOYW1 is the first phytoplasmal extrachromosomal DNA shown to express encoded genes.
authors.affiliation: Laboratory of Bioresource Technology, Graduate School of Frontier Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan1.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Nishigawa
authors.affiliation: Laboratory of Bioresource Technology, Graduate School of Frontier Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan1.
authors.forename: Shin-Ichi
authors.initials: SI
authors.lastname: Miyata
authors.affiliation: Laboratory of Bioresource Technology, Graduate School of Frontier Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan1.
authors.forename: Kenro
authors.initials: K
authors.lastname: Oshima
authors.affiliation: Laboratory of Bioresource Technology, Graduate School of Frontier Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan1.
authors.forename: Toshimi
authors.initials: T
authors.lastname: Sawayanagi
authors.affiliation: Laboratory of Bioresource Technology, Graduate School of Frontier Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan1.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Komoto
authors.affiliation: Laboratory of Plant Breeding and Genetics, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan2.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Kuboyama
authors.affiliation: National Agricultural Research Center, 3-1-1 Kannondai, Tsukuba, Ibaraki 305-8666, Japan3.
authors.forename: Izumi
authors.initials: I
authors.lastname: Matsuda
authors.affiliation: Koibuchi College of Agriculture, 5965 Koibuchi, Uchihara-cho, Higashi-ibaraki, Ibaraki 319-0323, Japan4.
authors.forename: Tsuneo
authors.initials: T
authors.lastname: Tsuchizaki
authors.affiliation: Laboratory of Bioresource Technology, Graduate School of Frontier Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan1.
authors.forename: Shigetou
authors.initials: S
authors.lastname: Namba
mesh_terms: D000595:Amino Acid Sequence; D002876:Chromosomes, Bacterial / Q000235:genetics*; D011723:Chrysanthemum cinerariifolium / Q000382:microbiology*; D003001:Cloning, Molecular; D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism*; D004261:DNA Replication; D004269:DNA, Bacterial / Q000235:genetics; D004268:DNA-Binding Proteins; D017791:Geminiviridae / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000382:microbiology; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D008973:Tenericutes / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D015534:Trans-Activators; C045264:replication initiator protein; D004265:DNA Helicases