title: Collagen and fibronectin binding in experimental staphylococcal osteomyelitis.
abstract: A new mouse model of locally induced osteomyelitis was used to study the importance for pathogenicity of the specific binding ability of Staphylococcus aureus to collagen and fibronectin. This method appears to be convenient, reproducible, and suitable for large-scale experiments. In contrast to previous studies in experimental arthritis and endocarditis models, no difference in infection rates was found between the strains deficient in binding to collagen compared with the corresponding adherence-proficient strains. However, fibronectin binding ability in this model, in contrast to the endocarditis model, is thought to enhance the microorganisms' capacity to establish an infection. Infections caused by the fibronectin-binding strain also are thought to be clinically more aggressive than those caused by the nonbinding strain. Specific adherence mechanisms are thought to be operative in the pathogenesis of biomaterial associated osteomyelitis, and an improved understanding of such mechanisms may have an important prophylactic and therapeutic impact.
authors.affiliation: Department of Surgery,  Huddinge University Hospital, Karolinska Institutet, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Johansson
authors.forename: J I
authors.initials: JI
authors.lastname: Flock
authors.forename: O
authors.initials: O
authors.lastname: Svensson
mesh_terms: D018829:Adhesins, Bacterial; D000704:Analysis of Variance; D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology; D001426:Bacterial Proteins / Q000378:metabolism; D001672:Biocompatible Materials / Q000737:chemistry; D001864:Bone Wires / Q000382:microbiology; D002352:Carrier Proteins / Q000378:metabolism; D016009:Chi-Square Distribution; D003094:Collagen / Q000378:metabolism*; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism*; D016023:Integrins / Q000378:metabolism; D051379:Mice; D008808:Mice, Inbred CBA; D010019:Osteomyelitis / Q000378:metabolism / Q000382:microbiology*; D038981:Receptors, Collagen; D013193:Stainless Steel / Q000737:chemistry; D013203:Staphylococcal Infections / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000145:classification / Q000254:growth & development / Q000378:metabolism*; D013499:Surface Properties; D013977:Tibia / Q000601:surgery
chemical_list: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D001672:Biocompatible Materials; D002352:Carrier Proteins; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D016023:Integrins; D038981:Receptors, Collagen; C078635:fibronectin-binding proteins, bacterial; D013193:Stainless Steel; D003094:Collagen