Strength of the Calpha H..O hydrogen bond of amino acid residues.
Although the peptide C(alpha)H group has historically not been thought to form hydrogen bonds within proteins, ab initio quantum calculations show it to be a potent proton donor. Its binding energy to a water molecule lies in the range between 1.9 and 2.5 kcal/mol for nonpolar and polar amino acids; the hydrogen bond (H-bond) involving the charged lysine residue is even stronger than a conventional OH..O interaction. The preferred H-bond lengths are quite uniform, about 3.32 A. Formation of each interaction results in a downfield shift of the bridging hydrogen's chemical shift and a blue shift in the C(alpha)H stretching frequency, potential diagnostics of the presence of such an H-bond within a protein.
Scheiner
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Department of Chemistry and Biochemistry, Utah State University, Logan 84322-0300, USA. scheiner@cc.usu.edu
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D000596:Amino Acids / Q000737:chemistry* / Q000378:metabolism; D002244:Carbon / Q000737:chemistry* / Q000378:metabolism; D003198:Computer Simulation; D006859:Hydrogen / Q000737:chemistry* / Q000378:metabolism; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D010100:Oxygen / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D013816:Thermodynamics
D000596:Amino Acids; D002244:Carbon; D006859:Hydrogen; D010100:Oxygen