title: Risk assessment for developing gliomas: a comparison of two cytogenetic approaches.
abstract: Chromosome instability (CIN) measured as chromosome aberrations has long been suggested as a cancer susceptibility biomarker. Conventional cytogenetic end-points are now being improved by combining molecular methods, which increases the sensitivity, specificity, and precision of the assay. In this study we examined both spontaneous and gamma-ray induced CIN in lymphocyte cultures from 51 previously untreated glioma patients and 51 age-, sex- and ethnicity-matched controls. CIN was assessed using two parallel methods: (1) the mutagen sensitivity (MS) assay and (2) the multicolor fluorescence in situ hybridization (FISH) assay. The frequency of spontaneous breaks was significantly higher in glioma patients (mean+/-S.D., 2.12+/-1.07) than in controls (1.24+/-0.86, P<0.001) when using the FISH assay but not the MS assay (0.019+/-0.02 and 0.019+/-0.01, respectively; P=0.915). Similarly, the frequency of induced chromatid breaks was significantly higher using the FISH assay (3.39+/-1.72) but not the MS assay (0.42+/-0.16) in the patients versus controls (2.08+/-1.18 and 0.37+/-0.15, respectively; P<0.001 and P=0.10, respectively). By using the median number of breaks in the controls as the cutoff value, we observed an odds ratio (ORs) of 5.13 (95% CI=2.23-12.1) for spontaneous and 4.86 (95% CI=2.08-11.4) for induced CIN using the FISH assay, whereas the ORs were 1.32 (95% CI=0.49-3.58) and 1.28 (95% CI=0.59-2.80) for spontaneous and induced CIN using the MS assay. There was also a significant increase in the frequency of hyperdiploid cells in the glioma cases which could only be detected using the FISH assay (OR=4.0, 95% CL=0.9-17.0). By combining both methods an estimated risk of 7.0 (95% CI=1.7-25.6) was observed. There was no correlation between the breaks detected by the two methods suggesting that each method is a measure of a different event. The results indicate that using the multicolor FISH assay for detection of CIN in peripheral blood lymphocytes in glioma patients is a more useful marker for risk assessment.
authors.affiliation: Department of Epidemiology, The University of Texas, M.D. Anderson Cancer Center, Box 189, Houston, TX 77030, USA.
authors.forename: R
authors.initials: R
authors.lastname: El-Zein
authors.forename: M L
authors.initials: ML
authors.lastname: Bondy
authors.forename: L E
authors.initials: LE
authors.lastname: Wang
authors.forename: M
authors.initials: M
authors.lastname: de Andrade
authors.forename: A J
authors.initials: AJ
authors.lastname: Sigurdson
authors.forename: J M
authors.initials: JM
authors.lastname: Bruner
authors.forename: A P
authors.initials: AP
authors.lastname: Kyritsis
authors.forename: V A
authors.initials: VA
authors.lastname: Levin
authors.forename: Q
authors.initials: Q
authors.lastname: Wei
mesh_terms: D000328:Adult; D016543:Central Nervous System Neoplasms / Q000235:genetics* / Q000473:pathology; D002869:Chromosome Aberrations; D005260:Female; D005820:Genetic Testing / Q000379:methods*; D005910:Glioma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male; D015162:Micronucleus Tests / Q000379:methods*; D008875:Middle Aged