Enzyme engineering: rational redesign versus directed evolution.
Enzyme engineering is undergoing the most profound and exciting transformation in its history (1). It promises unprecedented expansion in the scope and applications of modified or improved enzymes with desired physical and catalytic properties. Two complementary strategies are currently available: rational redesign (2-5) and directed evolution (6,7). Although both approaches have met with great success, each has limitations. In this article, the perspectives for these enzyme-engineering strategies are discussed briefly.
Chen
R
R
Dept of Biochemistry, College of Medicine, University of Saskatchewan, 107 Wiggins Road, Saskatoon, SK S7N 5E5, Canada. ridong.chen@monsanto.com
D004798:Enzymes / Q000737:chemistry* / Q000235:genetics*; D008967:Molecular Biology / Q000639:trends*; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction; D015202:Protein Engineering / Q000379:methods*
D004798:Enzymes