Isolation of Legionella anisa using an amoebic coculture procedure.
Conventional diagnostic tests for legionellosis were negative for a 61-year-old immunocompromised man with pneumonia. However, coculture of a sputum sample with Acanthamoeba polyphaga amoebae led to the recovery of Legionella anisa. This procedure may be a sensitive and convenient diagnostic method, especially for non-Legionella pneumophila species infections that can be diagnosed only by culture.
La Scola
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Unité des Rickettsies, CNRS UPRESA 6020, Faculté de Médecine, 13385 Marseille Cedex 05, France.
Mezi
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Weiller
P J
PJ
Raoult
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D000048:Acanthamoeba / Q000254:growth & development / Q000382:microbiology*; D000818:Animals; D006801:Humans; D007875:Legionella / Q000254:growth & development / Q000302:isolation & purification*; D007876:Legionellosis / Q000175:diagnosis / Q000382:microbiology*; D008297:Male; D008875:Middle Aged