Growth stimulation of Porphyromonas endodontalis by hemoglobin and protoporphyrin IX.
Porphyromonas endodontalis, like other Porphyromonas species, has a complex set of nutritional requirements. In addition to being an obligate anaerobe, the bacterium must be grown in a complex medium consisting of amino acids, reducing agents and heme compounds. P. endodontalis accumulates high concentrations of heme pigments to the extent that colonies appear black on blood agar. This accumulation of heme and the need for these compounds has been characterized as iron requirements by these species. However, in our studies, P. endodontalis demonstrated growth dependence on hemoglobin or protoporphyrin IX but not on free iron. Iron added to other heme compounds actually decreased growth stimulation by porphyrin-containing compounds. P. endodontalis actively transported free iron, but this process did not appear to be critical for growth. The maximum stimulation of growth by protoporphyrin IX, under conditions of iron deprivation, suggests that P. endodontalis requires the porphyrin moiety as a growth factor.
Zerr
M A
MA
College of Medicine, University of Iowa, Iowa City, USA.
Cox
C D
CD
Johnson
W T
WT
Drake
D R
DR
D001693:Biological Transport, Active; D002712:Chlorides; D003470:Culture Media; D003786:Dental Pulp Cavity / Q000382:microbiology; D005290:Ferric Compounds / Q000378:metabolism; D006418:Heme / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D017861:Porphyromonas / Q000254:growth & development* / Q000378:metabolism*; D011524:Protoporphyrins / Q000378:metabolism; D017262:Siderophores / Q000378:metabolism
D002712:Chlorides; D003470:Culture Media; D005290:Ferric Compounds; D006454:Hemoglobins; D011524:Protoporphyrins; D017262:Siderophores; D006418:Heme; C028025:protoporphyrin IX; D007501:Iron; C024555:ferric chloride