Highly sensitive, fluorescent transformation marker for Drosophila transgenesis.
The efficiency of transposon-mediated germline transformation is dependent on the transposon mobility in the host embryo, and on the detectability of the used transformation marker. Therefore, high susceptibility of the transformation marker to position effect suppression is a disadvantage. Here we present data that the eye-specific expression of green fluorescent protein, driven by the 3xP3-EGFP marker, outperforms the commonly used "mini"-white transformation marker in Drosophila germline transformation experiments: 3xP3-EGFP is more sensitive than "mini"-white in identifying transgenic individuals and reacts differently to position effect suppression. Therefore, 3xP3-EGFP offers an ideal marker for applications in functional genomics where as many gene loci as possible should be targeted in the genome of a specific organism, for example, as intended in the Drosophila gene disruption project. Furthermore, we give a detailed description of the embryonic and larval expression mediated by the 3xP3-EGFP marker. These pre-adult expression patterns, and the potentially universal applicability of the transformation marker also offer additional advantages for selecting transgenic individuals in organisms other than Drosophila. This will be of great interest to the field of evolutionary developmental biology and to modern pest management programs.
Horn
C
C
Lehrstuhl für Genetik, Universität Bayreuth, Universitätsstrasse 30 NWI, 95447 Bayreuth, Germany.
Jaunich
B
B
Wimmer
E A
EA
D000818:Animals; D004330:Drosophila / Q000235:genetics*; D005456:Fluorescent Dyes; D005819:Genetic Markers; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics; D012680:Sensitivity and Specificity; D014170:Transformation, Genetic; D019076:Transgenes
D005456:Fluorescent Dyes; D005819:Genetic Markers; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins