title: Dpp gradient formation in the Drosophila wing imaginal disc.
abstract: The secreted signaling protein Dpp acts as a morphogen to pattern the anterior-posterior axis of the Drosophila wing. Dpp activity is required in all cells of the developing wing imaginal disc, but the ligand gradient that supports this activity has not been characterized. Here we make use of a biologically active form of Dpp tagged with GFP to examine the ligand gradient. Dpp-GFP forms an unstable extracellular gradient that spreads rapidly in the wing disc. The activity gradient visualized by MAD phosphorylation differs in shape from the ligand gradient. The pMAD gradient adjusted to compartment size when this was experimentally altered. These observations suggest that the Dpp activity gradient may be shaped at the level of receptor activation.
authors.affiliation: European Molecular Biology Laboratory Meyerhofstr 1 69117, Heidelberg, Germany.
authors.forename: A A
authors.initials: AA
authors.lastname: Teleman
authors.forename: S M
authors.initials: SM
authors.lastname: Cohen
mesh_terms: D000818:Animals; D019521:Body Patterning / Q000235:genetics; D002478:Cells, Cultured; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D015151:Immunoblotting; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D008024:Ligands; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D018613:Microscopy, Confocal; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D021381:Protein Transport / Q000502:physiology; D011956:Receptors, Cell Surface / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012097:Repressor Proteins; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014921:Wings, Animal / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D029721:Drosophila Proteins; D049452:Green Fluorescent Proteins; D018398:Homeodomain Proteins; D019476:Insect Proteins; D008024:Ligands; D008164:Luminescent Proteins; D019869:Phosphatidylinositol 3-Kinases; D017346:Protein Serine-Threonine Kinases; D011956:Receptors, Cell Surface; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; C118082:brk protein, Drosophila; C064196:dpp protein, Drosophila; C085793:salm protein, Drosophila; C090169:tkv protein, Drosophila