Endothelial-like cells from the bovine placental cotyledon.
A cell-line was established from bovine placental cotyledon. When cultured in M199 with 10% fetal bovine serum, this cell-line had a doubling time of about 18 h. With immunohistochemistry, it was demonstrated that this cell-line expressed vimentin and angiotensin-converting enzyme (ACE). While both molecules are expressed in endothelial cells, ACE is usually considered to be a specific marker for endothelial cells. Furthermore, cells were shown to take up Dil-Ac-LDL (acetylated low-density lipoprotein labeled with 1,1'-dioctadecyl-3,3,3'-tetramethylindo-carbocyanine perchlorate). This characteristic feature has been used to identify endothelial cells. Finally, when cultured on matrigel, this cell-line formed tube-like structures similar to those formed by endothelial cells. Tube-formation on matrigel is a physiological property specific to endothelial cells. In conclusion, these three lines of evidence strongly suggest that this cell-line is endothelial cell in nature. Further studies using an endothelial cell-line from bovine placenta may help to elucidate the cause of bovine placental retention, a major cause for economic loss in bovine industry. Furthermore, an endothelial cell-line could be an important tool in research areas such as tissue remodeling, angiogenesis, and cancer.
Feng
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Department of Veterinary Clinical and Basic Medical Sciences, School of Veterinary Medicine, Purdue University, West Lafayette, Indiana 47907, USA.
Peter
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AT
Asem
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EK
D000818:Animals; D002232:Carbocyanines; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D003094:Collagen; D004338:Drug Combinations; D004727:Endothelium / Q000166:cytology*; D005456:Fluorescent Dyes; D006801:Humans; D007797:Laminin; D008077:Lipoproteins, LDL; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D010920:Placenta / Q000166:cytology*; D011509:Proteoglycans; D014746:Vimentin / Q000378:metabolism
D002232:Carbocyanines; D004338:Drug Combinations; D005456:Fluorescent Dyes; D007797:Laminin; D008077:Lipoproteins, LDL; D011509:Proteoglycans; D014746:Vimentin; C059485:matrigel; C024286:3,3'-dioctadecylindocarbocyanine; D003094:Collagen; D007703:Peptidyl-Dipeptidase A