Migrastatin, a new inhibitor of tumor cell migration from Streptomyces sp. MK929-43F1. Taxonomy, fermentation, isolation and biological activities.
A new compound, migrastatin, was isolated from a cultured broth of Streptomyces sp. MK929-43F1, as an inhibitor of tumor cell migration. It was purified by column chromatographies on silica gel and Sephadex LH-20 and HPLC. Migrastatin has the molecular formula of C27H39NO7 consisting of 14-membered macrolide and glutarimide moiety. It inhibited spontaneous migration of human esophageal cancer EC17 cells. Migration inhibitory activity of migrastatin was not dependent on cytotoxicity or inhibition of protein synthesis.
Nakae
K
K
Department of Applied Chemistry, Faculty of Science and Technology, Keio University, Yokohama, Japan.
Yoshimoto
Y
Y
Sawa
T
T
Homma
Y
Y
Hamada
M
M
Takeuchi
T
T
Imoto
M
M
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002633:Chemotaxis / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis; D004354:Drug Screening Assays, Antitumor; D004938:Esophageal Neoplasms; D005285:Fermentation; D006801:Humans; D007783:Lactones / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D018942:Macrolides; D008546:Melanoma, Experimental; D051379:Mice; D009369:Neoplasms / Q000473:pathology*; D010881:Piperidones / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D013302:Streptomyces / Q000145:classification / Q000254:growth & development / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D014945:Wound Healing / Q000187:drug effects
D000903:Antibiotics, Antineoplastic; D007783:Lactones; D018942:Macrolides; D010881:Piperidones; C418199:migrastatin; D012313:RNA; D004247:DNA