title: Classical swine fever virus: clinical, virological, serological and hematological findings after infection of domestic pigs and wild boars with the field isolate "Spante" originating from wild boar.
abstract: A classical swine fever virus (CSFV) field isolate originating from wild boar was investigated on its virulence in domestic pigs and wild boar. Three weaner pigs and two wild boars (yearlings) were intranasally inoculated with the isolate "Spante" and tested for clinical, virological, hematological and serological findings until day 31 after infection (p. i.). One day p. i. the piglets were put in contact to three sentinel pigs. During a period of 31 d neither the domestic pigs nor the wild boars showed clinical signs specific for CSF. Two infected weaner pigs became transiently viraemic, transmitted CSFV in nasal secretions, showed a slight leukopenia and reacted serologically positive. The contact infection resulted in a viraemia in two sentinel piglets on day 30. Only one contact animal developed antibodies. None of the wild boars became viraemic, excreted CSFV in nasal secretions or developed antibodies. The CSFV isolate "Spante" represents a low virulent virus. Referring to a significant higher percentage of virologically positive tissue samples after nested PCR compared with the virus isolation, persistence of CSFV is discussed.
authors.affiliation: Bundesforschungsanstalt für Viruskrankheiten der Tiere, Institut für Infektionsmedizin der Friedrich-Loeffler-Institute Insel Riems. volker.kaden@rie.bfav.de
authors.forename: V
authors.initials: V
authors.lastname: Kaden
authors.forename: U
authors.initials: U
authors.lastname: Ziegler
authors.forename: E
authors.initials: E
authors.lastname: Lange
authors.forename: J
authors.initials: J
authors.lastname: Dedek
mesh_terms: D000818:Animals; D000829:Animals, Domestic; D000835:Animals, Wild / Q000821:virology; D000914:Antibodies, Viral / Q000097:blood; D002460:Cell Line; D006691:Classical Swine Fever / Q000097:blood / Q000503:physiopathology* / Q000635:transmission; D006692:Classical Swine Fever Virus / Q000145:classification* / Q000302:isolation & purification; D008297:Male; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine / Q000821:virology*; D014766:Viremia / Q000503:physiopathology
chemical_list: D000914:Antibodies, Viral