title: Stabilisation of Salmonella vaccine vectors by the induction of trehalose biosynthesis.
abstract: The growth of an aroA mutant of Salmonella typhimurium (SL3261) in minimal medium containing 0.5 M NaCl resulted in the intracellular accumulation of 2.2 micromol trehalose/mg total protein. The vacuum drying of these bacteria in the presence of trehalose allowed the recovery of 35% of the viable cells that were present before drying. In contrast, bacteria cultured in control medium accumulated 0.4 micromol trehalose/mg total protein and only 5% of the viable cells were recovered after vacuum drying with trehalose. Similar results were obtained when S. typhimurium SL3261, expressing the vaccine antigen (F1-antigen) of Yersinia pestis, was cultured in minimal medium with or without 0.5 M NaCl and dried in the presence of trehalose. Although these results indicate the potential for trehalose stabilisation of vaccine strains of S. typhimiurium, growth in minimal medium containing 0.5 M NaCl resulted in the loss of invasion competence of the bacteria.
authors.affiliation: Defence Evaluation and Research Agency, CBD Porton Down, Wiltshire SP4 0JQ, Salisbury, UK.
authors.forename: H L
authors.initials: HL
authors.lastname: Bullifent
authors.forename: K
authors.initials: K
authors.lastname: Dhaliwal
authors.forename: A M
authors.initials: AM
authors.lastname: Howells
authors.forename: K
authors.initials: K
authors.lastname: Goan
authors.forename: K
authors.initials: K
authors.lastname: Griffin
authors.forename: C D
authors.initials: CD
authors.lastname: Lindsay
authors.forename: A
authors.initials: A
authors.lastname: Tunnacliffe
authors.forename: R W
authors.initials: RW
authors.lastname: Titball
mesh_terms: D000818:Animals; D002460:Cell Line; D005260:Female; D005964:Glucosyltransferases / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics; D022562:Salmonella Vaccines / Q000737:chemistry / Q000276:immunology*; D012486:Salmonella typhimurium / Q000254:growth & development / Q000276:immunology*; D014199:Trehalose / Q000096:biosynthesis*
chemical_list: D022562:Salmonella Vaccines; D014199:Trehalose; D005964:Glucosyltransferases; C023180:trehalose-6-phosphate synthase; C023178:trehalose-phosphatase; D010744:Phosphoric Monoester Hydrolases