title: Inhibition of hamster sperm acrosomal enzyme by gossypol is closely associated with the decrease in fertilization capacity.
abstract: To elucidate the mechanism of sterility induced by gossypol, we studied the relationship between the activities of acrosomal enzymes and their fertilizing capacity in the hamster. The results showed that the ability of spermatozoa to penetrate into bovine cervical mucus, hyperactivated motility (HAM) and fertility in vivo were significantly inhibited when spermatozoa were exposed to gossypol (2.5 microg - 60 microg/mL) for 15 min in vitro. Also, following administration of gossypol (12.5 mg/kg/day) for 6 weeks, sperm motility, HAM and rate of fertilization in vitro by the hamster cauda epididymal spermatozoa were significantly decreased and the extracts of testis delayed dispersion of the cumulus oophorus cells, suggesting that hyaluronidase and other acrosomal enzymes might be inhibited by gossypol. In addition, acrosin and arylsulfatase activities were also markedly inhibited. These data show that the inhibition of acrosin and arylsulfatase activities is the main cause of gossypol-induced infertility. The inhibition was dependent upon gossypol dose and the duration of administration. Thus, the assay of acrosin and arylsulfatase activities may provide a useful tool for monitoring sterility induced by gossypol.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30620, USA.
authors.forename: Y Y
authors.initials: YY
authors.lastname: Yuan
authors.forename: Q X
authors.initials: QX
authors.lastname: Shi
mesh_terms: D000176:Acrosin / Q000037:antagonists & inhibitors; D000177:Acrosome / Q000201:enzymology*; D000818:Animals; D001192:Arylsulfatases / Q000378:metabolism; D002417:Cattle; D002582:Cervix Mucus / Q000187:drug effects; D006224:Cricetinae; D005298:Fertility / Q000187:drug effects*; D006072:Gossypol / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D013737:Testis / Q000201:enzymology
chemical_list: D001192:Arylsulfatases; D000176:Acrosin; D006072:Gossypol