Comparison of the protection against neuronal injury by hypothalamic peptides and by dexamethasone.
The comparative study has been carried out on hypothalamic neurohormone (proline-rich polypeptides-PRP) and synthetic glucocorticoid dexamethasone (DEX) protective properties at the systemic (i/m) administration. Both background and evoked electrical activity (on n.ischiadicus stimulation) of single neurons in the lumbo-sacral part (laminae II-VI and VII-VIII by Rexed) and field potentials (FP) of spinal cord were recorded during acute experiments on intact spinal rats, subjected to Vipera Raddei (VR) venom intoxication, and chronic spinal cord trauma (hemisection). The action of PRP was characterized by the pronounced activation of the background activity (BA) with adaptive effect, depending on dose and initial level of BA, by results of the statistical analysis. A high effect is received from comparatively small doses. For comparison it was used strong glucocorticoid DEX, possessing single-directed but less expressed excitative action on investigated spinal cord neurons. The initial increase of BA frequency with subsequent depression was the typical symptom of venom influence. A protective effect of preliminary PRP injection is revealed on the succeeding VR venom influence. Use of PRP and DEX causes the increase of reduced activity of neurons on the injury side of animals with spinal cord hemisection. It provides the possibility of the therapeutic utilization. It was revealed considerably more expressed PRP action on neurodegenerative process connected to spinal cord injury (in comparison with DEX). The influence of hormones was compared in identical conditions of experiments on non-injured (control) and injured sides. Taking into consideration revealed protection characteristic of PRP and also the ability of snake venom to stabilize and to prolong its action combined with these preparations, the assumption is made on prospective use of the specified combination in clinical practice.
Galoyan
A A
AA
Buniatian Institute of Biochemistry NAS RA, Yerevan, The Republic of Armenia. galoyan@sci.am
Sarkissian
J S
JS
Kipriyan
T K
TK
Sarkissian
E J
EJ
Grigorian
Y K
YK
Sulkhanyan
R M
RM
Khachatrian
T S
TS
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D003655:Decerebrate State; D003907:Dexamethasone / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D005938:Glucocorticoids / Q000494:pharmacology*; D007031:Hypothalamus / Q000378:metabolism*; D007273:Injections, Intramuscular; D008297:Male; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010455:Peptides / Q000378:metabolism / Q000494:pharmacology*; D055232:Proline-Rich Protein Domains; D051381:Rats; D017208:Rats, Wistar; D013116:Spinal Cord / Q000187:drug effects* / Q000503:physiopathology; D013119:Spinal Cord Injuries / Q000503:physiopathology; D014757:Viper Venoms / Q000506:poisoning
D005938:Glucocorticoids; D018696:Neuroprotective Agents; D010455:Peptides; D014757:Viper Venoms; D003907:Dexamethasone