A single amino acid substitution in the ICP27 protein of herpes simplex virus type 1 is responsible for its resistance to leptomycin B.
Leptomycin B (LMB) is a specific inhibitor of Crm1-dependent nuclear export of proteins. The replication of herpes simplex virus (HSV) is normally highly sensitive to LMB; a resistant HSV variant, however, was isolated by serial passages of the virus. Analysis of marker transfer and viral DNA sequences revealed that a single amino acid substitution within the ICP27 gene is responsible for conferring this resistance.
Murata
T
T
Laboratory of Virology, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Showa-ku, Nagoya 466-8550, Japan.
Goshima
F
F
Koshizuka
T
T
Takakuwa
H
H
Nishiyama
Y
Y
D019943:Amino Acid Substitution; D000818:Animals; D002522:Chlorocebus aethiops; D004352:Drug Resistance, Microbial; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D018259:Herpesvirus 1, Human / Q000187:drug effects* / Q000235:genetics / Q000502:physiology*; D006801:Humans; D017874:Immediate-Early Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014709:Vero Cells; D014779:Virus Replication / Q000187:drug effects
D005231:Fatty Acids, Unsaturated; C057120:ICP27 protein, human herpesvirus 1; D017874:Immediate-Early Proteins; C038753:leptomycin B