[Analysis of sequence and polymorphism of R in S-type CMS maize].
The S-type CMS of maize was reported to be caused by a DNA region designated R in mitochondrial genomes. Through PCR using artificial primers, 1 kb DNA fragments were obtained from mitochondrial DNA of Tangxu and WB cytoplasm. The DNA sequences are almost the same as the sequence in R region of CMS-S. Using the DNA fragment from Tangxu cytoplasm as probe to scan the Mo17 CMS-J gene library, several positive clones appeared. The clone B39 contains a 6.7 kb BamHI fragment with R region. Nucleotide sequence analysis found two orfs including the chimeric orf77. Seventeen lines of maize under Mo17 and 77 nuclear background including N, T, C, S four types of cytoplasm were used for RFLP. Three types of R were discovered in S-type CMS, but not in C group. The R in N group was different from that in S group. T group had only part of R region. The Southern blot of Tangxü and Shuang cytoplasm in R regions showed different ratio of R in different nuclear backgrounds. The heterogeneity of R region suggests that the organization and rearrangement of R controlled by the nuclear background. R region seems to be involved in the mechanism of S group CMS. Varieties of structures of R, the ratio of three R types, different DNA sequences in R region of different CMS might be concerned with instability of S-type CMS.
Zhang
F D
FD
State Key Lab of Crop Genetic Improvement, Huazhong Agriculture University, Wuhan 430070, China.
Zheng
Y L
YL
D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000737:chemistry*; D018744:DNA, Plant / Q000737:chemistry*; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000235:genetics*; D011110:Polymorphism, Genetic; D003313:Zea mays / Q000235:genetics*
D004272:DNA, Mitochondrial; D018744:DNA, Plant