title: Qian-Kun-Nin, a Chinese herbal medicine formulation, attenuates mitochondrial oxidant stress in cardiomyocytes.
abstract: Qian-Kun-Nin is a Chinese herbal medicine formulation used for several indications, including the treatment of cardiovascular diseases. This formulation contains herbs which possess antioxidant properties. In this study, Qian-Kun-Nin's ability to confer protection to cardiomyocytes against reactive oxygen species (ROS) generated during mitochondrial electron transport inhibition was tested. The intracellular fluorescent probe 2',7'-dichlorofluorescin diacetate (DCFH-DA, sensitive to H(2)O(2) and hydroxyl radicals) was used to assess intracellular ROS, and propidium iodide (PI) was used to assess viability in cultured chick embryonic cardiomyocytes. Qian-Kun-Nin significantly attenuated oxidation of DCFH in cells exposed to the mitochondrial site III inhibitor, antimycin A, consistent with a decrease in oxidative stress. These attenuated oxidant levels were associated with improved cell survival. After antimycin A exposure, Qian-Kun-Nin decreased cell death from 51. 6+/-3.3% in untreated cells to 27.3+/-3.8% in treated cells at 2 h. We conclude that Qian-Kun-Nin attenuates oxidant stress and protects cells from lethal oxidant damage during mitochondrial electron transport inhibition, and thus its therapeutic potential in treating cardiovascular diseases may relate to its antioxidant properties.
authors.affiliation: Department of Medicine-Sections of Emergency Medicine and Pulmonary/Critical Care, University of Chicago, 60637, Chicago, IL, USA. cyuan@midway.uchicago.ed
authors.forename: Z
authors.initials: Z
authors.lastname: Shao
authors.forename: C
authors.initials: C
authors.lastname: Li
authors.forename: L B
authors.initials: LB
authors.lastname: Becker
authors.forename: T L
authors.initials: TL
authors.lastname: Vanden Hoek
authors.forename: P T
authors.initials: PT
authors.lastname: Schumacker
authors.forename: A S
authors.initials: AS
authors.lastname: Attele
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: J
authors.initials: J
authors.lastname: Xie
authors.forename: C
authors.initials: C
authors.lastname: Yuan
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D004579:Electron Transport / Q000187:drug effects; D020834:Ethnopharmacology; D008516:Medicine, Chinese Traditional; D008929:Mitochondria, Heart / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species
chemical_list: D004365:Drugs, Chinese Herbal; D017382:Reactive Oxygen Species; C425288:qian-kun-nin