Evaluation of PCR-based methods for the diagnosis of infection in bancroftian filariasis.
The value of the polymerase chain reaction (PCR) in the diagnosis of Wuchereria bancrofti infection was evaluated in comparison to microscopical examination of night blood smears, Nuclepore filtration, serology and ultrasonography. No correlation was found between PCR-based deoxyribonucleic acid (DNA) probing and serology. We did not find any evidence of free filarial DNA in either blood plasma or chylocoele fluid. We conclude that the 2 PCR-based techniques evaluated are not more sensitive than Nuclepore filtration for detection of W. bancrofti microfilaraemia, need at least 1 intact microfilaria in the volume of blood used for DNA extraction, and were much inferior to ultrasonography for detection of amicrofilaraemic adult worm carriers.
Dissanayake
S
S
Department of Microbiology, Faculty of Medicine, UAE University, PO Box 17666, Al Ain, United Arab Emirates. sendiss@uaeu.ac.ae
Rocha
A
A
Noroes
J
J
Medeiros
Z
Z
Dreyer
G
G
Piessens
W F
WF
D018509:DNA, Helminth / Q000097:blood; D005368:Filariasis / Q000175:diagnosis*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards*; D012680:Sensitivity and Specificity
D018509:DNA, Helminth