title: Study on free radicals and pancreatic fibrosis--pancreatic fibrosis induced by repeated injections of superoxide dismutase inhibitor.
abstract: The exact mechanisms of the development of pancreatic fibrosis are still unknown. To clarify the relationship between pancreatic fibrosis and free radicals, the effect of the administration of a superoxide dismutase (SOD) inhibitor, diethyldithiocarbamate (DDC), on pancreatic fibrosis in rats was studied. A single intraperitoneal injection of 500 mg/kg of DDC significantly reduced SOD activity and significantly increased lipid peroxidation products in the pancreas, showing no histologic changes of inflammation or necrosis. Repeated administration of 500 mg/kg DDC, twice a week, caused inter- and intralobular fibrosis with atrophy of acinar cells in the pancreas for at least 2 weeks without fibrosis of the liver and kidney. Administration of allopurinol showed preventive effects against DDC-induced pancreatic fibrosis. In conclusion, repeated administration of DDC, which caused pancreatic fibrosis, is a new experimental model of pancreatic fibrosis from the viewpoint of oxidative stress.
authors.affiliation: Second Department of Internal Medicine, Okayama University Medical School, Japan. nytm-m@terra.dti.ne.jp
authors.forename: N
authors.initials: N
authors.lastname: Matsumura
authors.forename: K
authors.initials: K
authors.lastname: Ochi
authors.forename: M
authors.initials: M
authors.lastname: Ichimura
authors.forename: T
authors.initials: T
authors.lastname: Mizushima
authors.forename: H
authors.initials: H
authors.lastname: Harada
authors.forename: M
authors.initials: M
authors.lastname: Harada
mesh_terms: D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D001284:Atrophy; D002908:Chronic Disease; D004050:Ditiocarb / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005355:Fibrosis / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D005609:Free Radicals / Q000378:metabolism*; D007274:Injections, Intraperitoneal; D015227:Lipid Peroxidation; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D010195:Pancreatitis / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D005609:Free Radicals; D008054:Lipid Peroxides; D000493:Allopurinol; D004050:Ditiocarb; D013482:Superoxide Dismutase