title: Low levels of hepatitis C virus RNA in blood of infected patients under maintenance haemodialysis with high-biocompatibility, high-permeability filters.
abstract: BACKGROUND
  Patients undergoing maintenance haemodialysis are often infected with hepatitis C virus, yet the clinical course of liver disease is usually mild. We investigated whether the hepatitis C virus viral load is influenced by the haemodialytic procedure and the type of dialyser.
  
  
  METHODS
  Hepatitis C virus-RNA levels were measured using a quantitative polymerase chain reaction assay in predialysis blood from 51 hepatitis C virus-infected patients dialysed with different membranes. Paired pre- and post-dialysis measurements were also obtained in 18 patients.
  
  
  RESULTS
  Patients dialysed using cuprammonium-regenerated cellulose membranes with low (cuprofan) or intermediate (cellulose acetate or diacetate) biocompatibility had higher pre-dialysis hepatitis C virus-RNA levels (p<0.05] compared to those dialysed with synthetic high-biocompatibility, high-permeability polymeric membranes (polyacrylonitrile, polysulfone, polymethylmethacrylate or polycarbonate). Hepatitis C virus-RNA levels were unrelated to the duration of haemodialysis and the presence of abnormal transaminases. A significant reduction (p=0. 04) of serum hepatitis C virus-RNA levels was observed after a single haemodialysis, particularly in patients with high pre-dialysis viral load. Patients with low pre-dialysis hepatitis C virus-RNA levels (<2. 5 x 10(3) copies/ml) exhibited only minimal changes following the procedure. Four patients with medium-high basal viral load switched from a low-biocompatibility/low-permeability to a high-biocompatibility/high permeability filter had a marked reduction of viraemia after three weeks, in one case followed by a new increase after return to the original filter.
  
  
  CONCLUSIONS
  Haemodialysis with high-biocompatibility/high-permeability filters in hepatitis C virus-infected patients is associated with low blood levels of hepatitis C virus-RNA. This finding may be of clinical relevance, especially in patients listed for kidney transplantation.
authors.affiliation: Department of Public Health and Chairs of Surgery, Tor Vergata University, Rome, Italy. angelico@med.uniroma2.it
authors.forename: M
authors.initials: M
authors.lastname: Angelico
authors.forename: M
authors.initials: M
authors.lastname: Morosetti
authors.forename: S
authors.initials: S
authors.lastname: Passalacqua
authors.forename: M G
authors.initials: MG
authors.lastname: Chiappini
authors.forename: S
authors.initials: S
authors.lastname: Botta
authors.forename: D
authors.initials: D
authors.lastname: Ombres
authors.forename: G
authors.initials: G
authors.lastname: Splendiani
authors.forename: C U
authors.initials: CU
authors.lastname: Casciani
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D016174:Hepacivirus / Q000235:genetics*; D006526:Hepatitis C / Q000097:blood / Q000150:complications; D006801:Humans; D007676:Kidney Failure, Chronic / Q000150:complications / Q000628:therapy; D008297:Male; D008875:Middle Aged; D012367:RNA, Viral / Q000097:blood*; D006435:Renal Dialysis / Q000295:instrumentation*; D019562:Viral Load; D014766:Viremia
chemical_list: D012367:RNA, Viral