Genomic organization of sheep TRDJ segments and their expression in the delta-chain repertoire in thymus.
cDNA sequences obtained from polymerase chain reaction products of reverse-transcribed RNA from sheep thymus showed the presence of a large number of members of the TRDV1 gene family. Some are TRDV1 genes also found in peripheral blood lymphocytes, while four genes had not been described so far. The cDNA sequences also showed extensive junctional diversity and a preferential usage of the three TRDJ elements. We characterized the genomic organization of the sheep TRDJ locus and detected a correlation between the nonrandom usage of TRDJ elements during development and their chromosomal order.
Massari
S
S
Dipartimento di Anatomia Patologica e di Genetica, University of Bari, Italy.
Antonacci
R
R
Lanave
C
C
Ciccarese
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D018076:DNA, Complementary; D015870:Gene Expression; D019676:Genes, T-Cell Receptor delta; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012756:Sheep / Q000235:genetics*; D013950:Thymus Gland
D018076:DNA, Complementary; D016692:Receptors, Antigen, T-Cell, gamma-delta