title: Human anti-scrub typhus rickettsia and rabbit anti-Proteus antibodies recognize similar epitope in the O-polysaccharide part of Proteus mirabilis OXK lipopolysaccharide.
abstract: In the Weil-Felix test, sera from patients infected with Orientia tsutsugamushi reacted with lipopolysaccharide (LPS) from Proteus mirabilis OXK strains. The O-polysaccharide of P. mirabilis OXK LPS consisted of pentasaccharide repeating units, with amidically-linked lysine residues. The lysine, linked to galacturonic residues, which plays an important role in the reaction with rabbit anti-OXK antibodies, was revealed with the aid of synthetic antigens. Using ELISA, immunoglobulin M antibodies from scrub typhus patients reacted with the O-specific polysaccharide of strain OXK LPS only. This reaction was inhibited by rabbit antibodies specific to the O-antigen of strain OXK LPS. Both human and rabbit antibodies may bind to similar epitopes on the O-polysaccharide part of P. mirabilis OXK LPS.
authors.affiliation: Microbiology and Virology Centre, Polish Academy of Sciences, Lódź.
authors.forename: W
authors.initials: W
authors.lastname: Kaca
authors.forename: K
authors.initials: K
authors.lastname: Amano
authors.forename: A Y
authors.initials: AY
authors.lastname: Chernyak
authors.forename: Y A
authors.initials: YA
authors.lastname: Knirel
mesh_terms: D000180:Acrylic Resins; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000918:Antibody Specificity; D002240:Carbohydrate Sequence; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D006241:Haptens / Q000276:immunology; D006462:Hemolytic Plaque Technique; D006603:Hexuronic Acids; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology; D008239:Lysine; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry / Q000276:immunology*; D012285:Orientia tsutsugamushi / Q000276:immunology*; D011513:Proteus mirabilis / Q000276:immunology*; D011817:Rabbits; D012612:Scrub Typhus / Q000276:immunology; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D000907:Antibodies, Bacterial; D000939:Epitopes; D006241:Haptens; D006603:Hexuronic Acids; D007075:Immunoglobulin M; D008239:Lysine; D019081:O Antigens; D000099166:Polyacrylamides; C007819:galacturonic acid