title: Angiotensin II as a trophic factor of white adipose tissue: stimulation of adipose cell formation.
abstract: White adipose tissue is known to contain the components of the renin-angiotensin system giving rise to angiotensin II (AngII). In vitro, prostacyclin is synthesized from arachidonic acid through the activity of cyclooxygenases 1 and 2 and is released from AngII-stimulated adipocytes. Prostacyclin, in turn, is able to favor adipocyte formation. Based upon in vivo and ex vivo experiments combined to immunocytochemical staining of glycerol-3-phosphate dehydrogenase (GPDH), an indicator of adipocyte formation, it is reported herein that AngII favors the appearance of GPDH-positive cells. In the presence of a cyclooxygenase inhibitor, this adipogenic effect is abolished, whereas that of (carba)prostacyclin, a stable analog of prostacyclin that bypasses this inhibition, appears unaltered. Taken together, these results are in favor of AngII acting as a trophic factor implicated locally in adipose tissue development. It is proposed that AngII enhances the formation of GPDH-expressing cells from preadipocytes in response to prostacyclin released from adipocytes.
authors.affiliation: Institut de Recherches Signalisation, Biologie du Développement et Cancer, Laboratoire Biologie du Développement du Tissu Adipeux, Centre de Biochimie, 06108 Nice, France.
authors.forename: P
authors.initials: P
authors.lastname: Saint-Marc
authors.forename: L P
authors.initials: LP
authors.lastname: Kozak
authors.forename: G
authors.initials: G
authors.lastname: Ailhaud
authors.forename: C
authors.initials: C
authors.lastname: Darimont
authors.forename: R
authors.initials: R
authors.lastname: Negrel
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects; D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D011464:Epoprostenol / Q000494:pharmacology; D005993:Glycerolphosphate Dehydrogenase / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D006720:Homozygote; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000804:Angiotensin II; D011464:Epoprostenol; D005993:Glycerolphosphate Dehydrogenase