Self-cleavage of p2Sp1 RNA with Mg2+ and non-ionic detergent (Brij 58).
The precursor of an RNA molecule from T4-infected E. coli cells (p2Sp1 RNA) has the capacity to cleave itself at specific positions [(UpA (139-140) and CpA (170-171)], within a putative loop and stem structure. This sequence-specific cleavage requires at least a monovalent cation and non-ionic detergents. We studied the self-cleavage reaction of an RNA fragment (GUUUCGUACAAAC) (R1) with the sequence corresponding to the p2Sp1 RNA in the presence of Mg2+ and non-ionic detergents. It requires Mg2+ and is aided by a non-ionic detergent, Brij 58. The cleavage reaction is time, temperature, and pH-dependent. The cleavage occurs at the phosphodiester bond between UpA and CpA on the RNA fragment (GUUUCGUACAAAC) (R1). Furthermore, the maximum of cleavage of R1 occurs at a very low Mg2+ concentration (< or = 5 mM).
Hosaka
H
H
Department of Industrial Chemistry and High Technology Research Center, Chiba Institute of Technology, Narashino, Japan.
Hosono
K
K
Kawai
G
G
Takai
K
K
Takaku
H
H
D002592:Cetomacrogol / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000737:chemistry*; D009843:Oligoribonucleotides / Q000138:chemical synthesis* / Q000378:metabolism; D012322:RNA Precursors / Q000737:chemistry / Q000378:metabolism*; D013501:Surface-Active Agents / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors
D009843:Oligoribonucleotides; D012322:RNA Precursors; D013501:Surface-Active Agents; D002592:Cetomacrogol; D008274:Magnesium