title: Binary typing of Staphylococcus aureus strains through reversed hybridization using digoxigenin-universal linkage system-labeled bacterial genomic DNA.
abstract: A novel binary typing (BT) procedure, based on reversed hybridization of digoxigenin-universal linkage system-labeled bacterial DNA to strip-immobilized probes, is presented. Chromogenic detection of hybrids was performed. Staphylococcus aureus isolates (n = 20) were analyzed to establish the feasibility of BT. A technically simple and fast procedure has been developed for application in routine microbiology laboratories.
authors.affiliation: Department of Medical Microbiology & Infectious Diseases, Erasmus University Medical Center Rotterdam, 3015 GD Rotterdam, The Netherlands. vanleeuwen@bacl.azr.nl
authors.forename: W
authors.initials: W
authors.lastname: van Leeuwen
authors.forename: C
authors.initials: C
authors.lastname: Libregts
authors.forename: M
authors.initials: M
authors.lastname: Schalk
authors.forename: J
authors.initials: J
authors.lastname: Veuskens
authors.forename: H
authors.initials: H
authors.lastname: Verbrugh
authors.forename: A
authors.initials: A
authors.lastname: van Belkum
mesh_terms: D015373:Bacterial Typing Techniques; D002863:Chromogenic Compounds; D015342:DNA Probes / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics*; D004076:Digoxigenin; D016521:Electrophoresis, Gel, Pulsed-Field; D016680:Genome, Bacterial; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000379:methods; D019105:Random Amplified Polymorphic DNA Technique; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics
chemical_list: D002863:Chromogenic Compounds; D015342:DNA Probes; D004269:DNA, Bacterial; D004076:Digoxigenin