Characterization of the promoter of PRS1 in Saccharomyces cerevisiae identifies three regions potentially involved in control of expression.
The transcription initiation site of the Saccharomyces cerevisiae PRS1 gene was mapped at -179 bp. Measurement of beta-galactosidase activity of the successively deleted PRS1 promoter linked to lacZ and integrated at the ura3 locus defined three DNA regions involved in the control of PRS1 expression. Gel shift analysis confirmed the data.
Hernando
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Y
Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, United Kingdom.
Carter
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AT
Sickinger
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S
Schweizer
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M
D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D017930:Genes, Reporter; D007763:Lac Operon; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011993:Recombinant Fusion Proteins; D012268:Ribose-Phosphate Pyrophosphokinase / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D017384:Sequence Deletion; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D012268:Ribose-Phosphate Pyrophosphokinase