title: Activation of blood coagulation factor IX by gingipains R, arginine-specific cysteine proteinases from Porphyromonas gingivalis.
abstract: The effect of two arginine-specific cysteine proteinases (gingipains R) from Porphyromonas gingivalis, an aetiological factor of adult periodontitis, on the activation of human factor IX was investigated in the presence of ethylene glycol, an activity enhancer of activated factor IX (factor IXa), with the use of a fluorogenic oligopeptide substrate. Each gingipain R rapidly activated factor IX but the 95 kDa proteinase complex (HRgpA) that contains both haemagglutinin/adhesion and catalytic domains was 2.4-fold more efficient than the single-chain 50 kDa gingipain R (RgpB), which has only a catalytic domain. SDS/PAGE and N-terminal sequence analysis of factor IX digestion fragments indicated that, like all endogenous activators, gingipains R also produce factor IXabeta via an IXa intermediate. Significantly, phospholipids augmented the activation of factor IX by HRgpA but not by RgpB in a Ca(2+)-dependent manner. In the presence of both cofactors the kinetic efficiency of HRgpA to activate factor IX (k(cat)/K(m)=1.9x10(6) M(-1).s(-1)) was 8.5-fold higher than that of RgpB (k(cat)/K(m)=2.3x10(5) M(-1).s(-1)) and double that of the factor VIIa-tissue factor complex, but 8-fold lower than that for factor XIa. A comparison of the relative activation rates of factor IX, factor X and prothrombin directly in plasma by HRgpA suggests a significant contribution for factor IX conversion in blood coagulation induced by gingipains R. Taken together, gingipains R are the first-reported activators of factor IX of bacterial origin. By this effect they could be involved in the production of thrombin as well as the subsequent generation of prostaglandins and interleukin 1, all of which have been found to be associated with the development and progression of periodontitis.
authors.affiliation: Division of Molecular Pathology, Department of Neuroscience and Immunology, Kumamoto University Graduate School of Medical Sciences, 2-2-1 Honjo, Kumamoto 860-0811, Japan. taka@kaiju.medic.kumamoto-u.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Imamura
authors.forename: S
authors.initials: S
authors.lastname: Tanase
authors.forename: T
authors.initials: T
authors.lastname: Hamamoto
authors.forename: J
authors.initials: J
authors.lastname: Potempa
authors.forename: J
authors.initials: J
authors.lastname: Travis
mesh_terms: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins / Q000494:pharmacology*; D003546:Cysteine Endopeptidases / Q000302:isolation & purification / Q000494:pharmacology*; D005164:Factor IX / Q000378:metabolism*; D015949:Factor IXa / Q000378:metabolism; D005170:Factor X / Q000378:metabolism; D000080867:Gingipain Cysteine Endopeptidases; D006388:Hemagglutinins / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D007527:Isoenzymes / Q000494:pharmacology; D007700:Kinetics; D010743:Phospholipids / Q000494:pharmacology; D016966:Porphyromonas gingivalis / Q000737:chemistry / Q000201:enzymology*; D011516:Prothrombin / Q000378:metabolism
chemical_list: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D000080867:Gingipain Cysteine Endopeptidases; D006388:Hemagglutinins; D007527:Isoenzymes; D010743:Phospholipids; D011516:Prothrombin; D005164:Factor IX; D005170:Factor X; D015949:Factor IXa; D003546:Cysteine Endopeptidases