title: Evaluation of the Triage Micro Parasite Panel for detection of Giardia lamblia, Entamoeba histolytica/Entamoeba dispar, and Cryptosporidium parvum in patient stool specimens.
abstract: A study comparing the Triage Micro Parasite Panel (Biosite Diagnostics, Inc., San Diego, Calif.) to conventional O&P examination (O&P) was performed using patient fecal specimens. Five hundred twenty-three stool samples were compared. Nineteen specimens were found to be positive by Triage, and 29 were found to be positive by O&P. Seven specimens were positive for Giardia lamblia, four were positive for Entamoeba histolytica/E. dispar, and three were positive for Cryptosporidium parvum as determined by both methods. There was one false positive by Triage (C. parvum) and four false negatives by O&P (two G. lamblia, one E. histolytica/E. dispar, and one C. parvum). The Triage test accurately detected all 18 specimens that contained one of the three organisms that it was designed to detect. The Triage test is a rapid, easy-to-use enzyme immunoassay for the detection of G. lamblia, E. histolytica/E. dispar, and C. parvum in fresh or fresh-frozen fecal specimens. These data suggest that the Triage test can be used as a screen for the immediate testing of stool specimens for these three pathogenic parasites. If Triage test results are negative, O&P can be performed if parasitic infections other than G. lamblia, E. histolytica/E. dispar, or C. parvum are suspected.
authors.affiliation: Department of Pathology and Laboratory Medicine, Mount Sinai Medical Center, Miami Beach, Florida 33140, USA. sue-sharp@msmc.com
authors.forename: S E
authors.initials: SE
authors.lastname: Sharp
authors.forename: C A
authors.initials: CA
authors.lastname: Suarez
authors.forename: Y
authors.initials: Y
authors.lastname: Duran
authors.forename: R J
authors.initials: RJ
authors.lastname: Poppiti
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis; D003457:Cryptosporidiosis / Q000175:diagnosis / Q000469:parasitology; D016785:Cryptosporidium parvum / Q000302:isolation & purification; D004747:Entamoeba / Q000302:isolation & purification; D004748:Entamoeba histolytica / Q000302:isolation & purification; D004749:Entamoebiasis / Q000175:diagnosis / Q000469:parasitology; D005243:Feces / Q000469:parasitology*; D016829:Giardia lamblia / Q000302:isolation & purification; D005873:Giardiasis / Q000175:diagnosis / Q000469:parasitology; D006801:Humans; D007124:Immunoenzyme Techniques / Q000191:economics; D007411:Intestinal Diseases, Parasitic / Q000175:diagnosis* / Q000469:parasitology; D011528:Protozoan Infections / Q000175:diagnosis* / Q000469:parasitology; D011933:Reagent Kits, Diagnostic / Q000191:economics; D012680:Sensitivity and Specificity
chemical_list: D000953:Antigens, Protozoan; D011933:Reagent Kits, Diagnostic