[Effect of topical pranoprofen on the lipoxygenase metabolism of the arachidonic and in endotoxin-induced uveitis].
OBJECTIVE
To study the antiinflammatory effect of topical pranoprofen on the lipoxygenase metabolism of the arachidonic acid in albino rabbits.


METHODS
Endotoxin-induced uveitis (EIU) was produced in albino rabbits by intravitreal injection in the right eye with 10 microg of Salmonella typhymurium lipopolysacharide A in 5 microl saline solution. We have used 5 groups of 12 animals each. Control group (G-I) was injected with 5 microl of saline solution and 5 microl of ET solution were injected in the remaining groups. Groups III, IV and V were treated with topical pranoprofen 2 hours before intravitreal injection, immediately after and every 6, 4 and 2 hours respectively. The animals were sacrificed 24 hours after the ET administration. Cellular and B4 leukotrien concentration in the aqueous humour was determined.


RESULTS
The groups treated with pranoprofen showed a significant decrease in the cellular concentration in relation to the group of endotoxin (G-II). We did not observe any difference in the B4 leukotriene concentration between ET group and topical pranoprofen groups.


CONCLUSIONS
Topical pranoprofen has not increased the lipoxygenase metabolism of the arachidonic acid.
Torrón
C
C
Servicio de Oftalmología, Hospital Miguel Servet, Diputación General de Aragón, Zaragoza, España.
Ferrer
E
E
Ruiz-Moreno
O
O
Cuevas
R
R
Pérez
S
S
Alabart
J L
JL
Honrubia
F M
FM
D000287:Administration, Topical; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use*; D016718:Arachidonic Acid / Q000378:metabolism*; D001578:Benzopyrans / Q000627:therapeutic use*; D004353:Drug Evaluation, Preclinical; D004731:Endotoxins; D008084:Lipoxygenase / Q000378:metabolism*; D011422:Propionates / Q000627:therapeutic use*; D011817:Rabbits; D014605:Uveitis / Q000209:etiology / Q000378:metabolism*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D001578:Benzopyrans; D004731:Endotoxins; D011422:Propionates; D016718:Arachidonic Acid; C043940:pyranoprofen; D008084:Lipoxygenase