title: Arabidopsis loss-of-function mutant in the lysine pathway points out complex regulation mechanisms.
abstract: In plants, the amino acids lysine, threonine, methionine and isoleucine have L-aspartate-beta-semialdehyde (ASA) as a common precursor in their biosynthesis pathways. How this ASA precursor is dispersed among the different pathways remains vague knowledge. The proportional balances of free and/or protein-bound lysine, threonine, isoleucine and methionine are a function of protein synthesis, secondary metabolism and plant physiology. Some control points determining the flux through the distinct pathways are known, but an adequate explanation of how the competing pathways share ASA in a fine-tuned amino acid biosynthesis network is yet not available. In this article we discuss the influence of lysine biosynthesis on the adjacent pathways of threonine and methionine. We report the finding of an Arabidopsis thaliana dihydrodipicolinate synthase T-DNA insertion mutant displaying lower lysine synthesis, and, as a result of this, a strongly enhanced synthesis of threonine. Consequences of these cross-pathway regulations are discussed.
authors.affiliation: Laboratorium voor Plantengenetica, Instituut voor Moleculaire Biologie, Vrije Universiteit Brussel, Paardenstraat 65, B-1640, Sint Genesius Rode, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Craciun
authors.forename: M
authors.initials: M
authors.lastname: Jacobs
authors.forename: M
authors.initials: M
authors.lastname: Vauterin
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D017353:Gene Deletion; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D006836:Hydro-Lyases / Q000235:genetics* / Q000378:metabolism; D008239:Lysine / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic
chemical_list: D000596:Amino Acids; D001224:Aspartic Acid; C006644:aspartic semialdehyde; D005966:Glucuronidase; D006836:Hydro-Lyases; C020040:4-hydroxy-tetrahydrodipicolinate synthase; D008239:Lysine