The effects of cryopreservation regimens on the morphology of human ovarian tissue.
Human ovarian tissue consisting of stroma, pre-granulosa cells and oocytes, has been frozen using a variety of cooling rates and dehydration regimens. The differential survival of the various cell types under these conditions highlights the difficulty in defining optimum protocols for the cryopreservation of multicellular tissue.
Gook
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DA
Reproductive Biology Unit, Royal Womens Hospital, Carlton, Victoria, Australia. gookd@cryptic.rch.unimelb.edu.au
Edgar
D H
DH
Stern
C
C
D002470:Cell Survival / Q000187:drug effects; D015925:Cryopreservation / Q000379:methods* / Q000592:standards; D003890:Desiccation / Q000379:methods; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005260:Female; D006107:Granulosa Cells / Q000166:cytology; D006801:Humans; D009865:Oocytes / Q000166:cytology; D010053:Ovary / Q000166:cytology*; D011247:Pregnancy; D019946:Propylene Glycol / Q000494:pharmacology; D017154:Stromal Cells / Q000166:cytology; D013395:Sucrose / Q000494:pharmacology; D013696:Temperature
D013395:Sucrose; D019946:Propylene Glycol; D004121:Dimethyl Sulfoxide