Widespread calcium deposits, as detected using the alizarin red S technique, in the nervous system of rats treated with dimethyl mercury.
It has been reported that the alizarin red S technique may be used to visualize both intracellular and extracellular calcium deposits. Using this method histologic observations of the nervous system were made in rats that were given dimethyl mercury at 5 mg/kg per day for 12 consecutive days, and killed on days 1, 4, 7, 10, 12, 24, 32, 49, 100 and 140 (day 0 was the day that the final dose was administered). Neuronal degeneration with calcium deposition was found in the nervous system from day 4 onward. In the cerebellum alizarin red S-positive granules became gradually larger with time after dimethyl mercury administration, and large calcospherites were observed from day 32 onward. In contrast, the visualization of calcium deposits in the cerebral cortex was restricted to days 10-12. Calcium deposits were found in the ascending axons of the dorsal root ganglion neurons (dorsal fascicles of the spinal cord), but not in their perikarya. These findings suggest that widespread calcium deposition could occur in the nervous system following dimethyl mercury exposure, and that in the rat the mechanism of calcium deposition differs depending upon the brain region.
Mori
F
F
Department of Neuropathology, Institute of Brain Science, Hirosaki University School of Medicine, Japan. neuropal@cc.hirosaki-u.ac.jp
Tanji
K
K
Wakabayashi
K
K
D000818:Animals; D000880:Anthraquinones; D002118:Calcium / Q000032:analysis*; D004305:Dose-Response Relationship, Drug; D005260:Female; D020262:Mercury Poisoning, Nervous System / Q000473:pathology*; D008767:Methylmercury Compounds / Q000633:toxicity*; D008854:Microscopy, Electron; D009410:Nerve Degeneration / Q000473:pathology*; D009420:Nervous System / Q000473:pathology* / Q000648:ultrastructure; D009474:Neurons / Q000473:pathology* / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar
D000880:Anthraquinones; D008767:Methylmercury Compounds; C004468:Alizarin Red S; C073972:dimethyl mercury; D002118:Calcium