[The characteristics of Bacillus thuringiensis strain YBT833 and its transformants that containing different ICP genes].
Four different transformants were selected by transferring cry1C into Bacillus thuringiensis strain YBT833. Southern blot and Plasmid profiles results all proved that cry1C was transferred into strain YBT833. However, it was found by PCR analysis that transformant YBT833-1 kept all indigenous ICP(insecticidal crystal protein) genes of strain YBT833 while transformant YBT833-2 lost cry1Ab, and transformant YBT833-3 lost all ICP genes. SDS-PAGE showed that transformants of YBT833-1, YBT833-2 and YBT833 all had 130 kDa and 65 kDa peptide bands, but transformant YBT833-3 did not have 65 kDa peptide band. Bioassay results showed that the toxicities of all transformants to Spodoptera exigua, Heliothis armigera and Plutella xylostella were lower than that of strain YBT833, except the toxicity of transformant YBT833-2 to P. xylostella which was obviously higher than that of YBT833.
Lu
S Q
SQ
Department of Microbial Science and Technology, Huazhong Agricultural University, Key Laboratory of Agro-microbiology, Ministry of Agriculture, Wuhan 430070, China.
Liu
Z D
ZD
Yu
Z N
ZN
D001413:Bacillus thuringiensis / Q000235:genetics*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins; D004731:Endotoxins / Q000235:genetics*; D006460:Hemolysin Proteins; D014170:Transformation, Genetic
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis