title: PCR analysis of cry7 genes in Bacillus thuringiensis by the five conserved blocks of toxins.
abstract: An alternative PCR analysis to screen for cry7 genes is proposed, based on the five conserved blocks of amino acids of Bacillus thuringiensis toxins and their encoding DNA sequences. A complete set of five primers was constructed, four direct and one reverse, yielding four specific amplicons. Modified profiles can identify new cry genes.
authors.affiliation: Department of Life Sciences, Ben-Gurion University of the Negev, P.O. Box 653, Be'er-Sheva 84105, Israel. ariehz@bgumail.bgu.ac.il
authors.forename: E
authors.initials: E
authors.lastname: Ben-Dov
authors.forename: R
authors.initials: R
authors.lastname: Manasherob
authors.forename: A
authors.initials: A
authors.lastname: Zaritsky
authors.forename: Z
authors.initials: Z
authors.lastname: Barak
authors.forename: Y
authors.initials: Y
authors.lastname: Margalith
mesh_terms: D000595:Amino Acid Sequence; D001413:Bacillus thuringiensis / Q000235:genetics*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics*; D017124:Conserved Sequence; D017931:DNA Primers; D004731:Endotoxins / Q000235:genetics*; D005798:Genes, Bacterial; D006460:Hemolysin Proteins; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D017931:DNA Primers; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis