Dendritic cells conditionally transformed by v-relER oncogene express lymphoid marker genes.
The initiation of primary immune responses is the key function of specialized antigen presenting cells, the dendritic cells (DC). DC of myeloid origin capture antigens in tissues, migrate to lymphoid organs and stimulate T cell responses. A subset of DC has been described which expresses lymphoid determinants and has potential regulatory functions. Conditional transformation of chicken bone marrow progenitors with v-relER, a v-rel estrogen receptor (ER) fusion gene, allows expansion of progenitors that can be induced to differentiate into DC in vitro. In this paper we describe that v-relER cells exhibit both myeloid and lymphoid surface markers, while B cell, T cell and NK (natural killer)-specific surface markers are absent. v-relER DC express, however, cytoplasmic CD3 protein and mRNA for CD8alpha and the lymphoid transcription factor GATA-3. These data suggest that v-relER DC might be related to the lymphoid subset of DC described in mammals.
Madruga
J
J
Max-Delbrück-Center for Molecular Medicine, Berlin, Germany.
Briegel
K
K
Diebold
S
S
Boehmelt
G
G
Vogl
F
F
Zenke
M
M
Vogel
F
F
D000818:Animals; D015415:Biomarkers; D017252:CD3 Complex / Q000235:genetics; D016827:CD8 Antigens / Q000235:genetics; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic; D002645:Chickens; D003593:Cytoplasm / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics; D003713:Dendritic Cells / Q000276:immunology*; D050990:GATA3 Transcription Factor; D015870:Gene Expression; D006412:Hematopoietic Stem Cells / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D020572:Oncogene Proteins v-rel / Q000235:genetics / Q000276:immunology*; D011960:Receptors, Estrogen / Q000235:genetics / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D015534:Trans-Activators / Q000235:genetics
D015415:Biomarkers; D017252:CD3 Complex; D016827:CD8 Antigens; D004268:DNA-Binding Proteins; D050990:GATA3 Transcription Factor; D000949:Histocompatibility Antigens Class II; D020572:Oncogene Proteins v-rel; D011960:Receptors, Estrogen; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators