[Antitumor activities in vivo of interleukin-12 gene modified dendritic cells in murine models].
OBJECTIVE
To study the antitumor activities of the mIL-12 transfected and acid-eluted peptide sensitized dendritic cells (DC) in murine H22 liver cancer model.


METHODS
The murine bone marrow derived DC were transfected with the recombinant adenovirus (Ad mIL-12) containing the mIL-12 gene and control virus AdBGFP. The peptides on the surface of the murine H22 cell line were eluted with mild acid buffer and then sensitized the transfected DC. The experimental animals were immunized with the differently disposed DC and the state of tumorgenesis and tumor growth was observed.


RESULTS
The tumor growth and weight in the group immunized with mIL-12 transfected and acid-eluted peptide sensitized DC were significantly lower than those of control group (P<0.05).


CONCLUSIONS
The mIL-12 transfected and acid-eluted peptide sensitized DC can induce obvious antitumor activities in the murine liver cancer models and may suggest new strategies for constructing new type of DC vaccine for liver cancer.
Liu
B
B
Liver Cancer Institute, Shanghai Medical University, Shanghai 200032, China.
Ye
S
S
He
P
P
Xue
Q
Q
Gao
D
D
Tang
Z
Z
D000818:Animals; D019496:Cancer Vaccines / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D018664:Interleukin-12 / Q000235:genetics*; D008114:Liver Neoplasms, Experimental / Q000628:therapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D014162:Transfection
D019496:Cancer Vaccines; D018664:Interleukin-12