Endocytosis and the development of cell polarity in yeast require a dynamic F-actin cytoskeleton.
Studies using drugs that cause the disassembly of filamentous actin (F-actin) have demonstrated the importance of an intact actin cytoskeleton for polarised secretion by yeast cells [1,2]. To address the level of dynamic turnover needed for such processes, however, drugs or mutants that confer stabilising properties on F-actin are needed. Jasplakinolide is the only readily available drug that stabilises F-actin structures both in vivo and in vitro [3-6]. Yeast strains have been generated in which two of the ABC multidrug resistance transporter genes have been deleted, rendering normally jasplakinolide-resistant yeast cells sensitive to its effects. Treatment of these cells with jasplakinolide caused rapid and dramatic effects on the actin cytoskeleton, resulting in the accumulation of single large actin structures in cells. These structures, however, still contained components that are normally associated with cortical actin patches. A dynamic actin cytoskeleton was found to be critical for the generation of cell polarity and endocytosis.
Ayscough
K R
KR
Institute of Biomedical and Life Sciences, Division of Biochemistry and Molecular Biology, Davidson Building, University of Glasgow, Glasgow G12 8QQ, UK. k.ayscough@bio.gla.ac.uk
D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D000199:Actins / Q000378:metabolism*; D000935:Antifungal Agents / Q000494:pharmacology*; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D016764:Cell Polarity; D003599:Cytoskeleton / Q000187:drug effects* / Q000378:metabolism*; D047630:Depsipeptides; D004705:Endocytosis / Q000502:physiology*; D005456:Fluorescent Dyes / Q000378:metabolism; D007546:Isoquinolines / Q000378:metabolism; D008840:Microfilament Proteins / Q000378:metabolism; D008856:Microscopy, Fluorescence; D010456:Peptides, Cyclic / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*; D013844:Thiazoles / Q000494:pharmacology; D053778:Thiazolidines; D020846:cdc42 GTP-Binding Protein, Saccharomyces cerevisiae / Q000378:metabolism
D018528:ATP-Binding Cassette Transporters; D000199:Actins; D000935:Antifungal Agents; D019086:Bridged Bicyclo Compounds, Heterocyclic; D047630:Depsipeptides; D005456:Fluorescent Dyes; D007546:Isoquinolines; D008840:Microfilament Proteins; D010456:Peptides, Cyclic; D013844:Thiazoles; D053778:Thiazolidines; C057531:jasplakinolide; C017475:lucifer yellow; D020846:cdc42 GTP-Binding Protein, Saccharomyces cerevisiae; C037067:latrunculin A