title: Canine visceral leishmaniasis on Margarita Island (Nueva Esparta, Venezuela).
abstract: The frequency of American visceral leishmaniasis affecting humans on Margarita Island, Venezuela, has increased in recent years, and infected dogs appear to constitute the principal source of infection. ELISA tests with Leishmania donovani promastigotes and rK39 antigen from L. chagasi in serum from 541 dogs were positive in 33.1% and 21.6% of the samples, respectively. A second blood sample taken from 50 animals after 8-10 months revealed an increase from 24% to 40% of ELISA positivity to both antigens, suggesting high susceptibility and transmission in the canine population. Among 42 serologically positive dogs, 33% of which showed clinical signs of disease, 79% were positive in polymerase chain reactions using primers specific for the L. donovani complex. Control measures including epidemiological hypersurveillance, the humane sacrifice of infected dogs, and rapid diagnosis and treatment of human cases have been initiated.
authors.affiliation: Instituto de Biomedicina, Universidad Central de Venezuela/Ministerio de Sanidad y Asistencia Social, Apartado 4043, Caracas 1010A, Venezuela. ozerpa@telcel.net.ve
authors.forename: O
authors.initials: O
authors.lastname: Zerpa
authors.forename: M
authors.initials: M
authors.lastname: Ulrich
authors.forename: E
authors.initials: E
authors.lastname: Negrón
authors.forename: N
authors.initials: N
authors.lastname: Rodríguez
authors.forename: M
authors.initials: M
authors.lastname: Centeno
authors.forename: V
authors.initials: V
authors.lastname: Rodríguez
authors.forename: R M
authors.initials: RM
authors.lastname: Barrios
authors.forename: D
authors.initials: D
authors.lastname: Belizario
authors.forename: S
authors.initials: S
authors.lastname: Reed
authors.forename: J
authors.initials: J
authors.lastname: Convit
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000453:epidemiology* / Q000469:parasitology; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D007898:Leishmaniasis, Visceral / Q000453:epidemiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000379:methods; D014687:Venezuela / Q000453:epidemiology