Fine particles of widely different composition have an adjuvant effect on the production of allergen-specific antibodies.
Diesel exhaust particles (DEP) are reported to increase the specific IgE response to allergens, and results from our laboratory suggest that the particle core of DEP contribute to this adjuvant activity. The purpose of the present study was to explore further the adjuvant effect of particles per se, that is particles by themselves. NIH/Ola mice were given two intraperitoneal injections with ovalbumin (OVA; 10 microg) alone or OVA in combination with PSP, polytetrafluoroethylene (teflon), titanium dioxide (TiO(2)) or amorphous silica particles (2.8x10(10)-2.8x10(12)). Blood samples were drawn 7 days after the last injection, and serum levels of allergen-specific and total IgE and IgG2a were measured. All types of particles gave increased levels of allergen-specific IgE and IgG2a. Similar results were obtained after intranasal or intratracheal instillation with OVA plus PSP or silica. Our results indicate that fine particles of widely different composition may have an adjuvant effect on the production of allergen-specific antibodies.
Granum
B
B
Department of Environmental Medicine, National Institute of Public Health, PO Box 4404 Nydalen, N-0403, Oslo, Norway. berit.granum@folkehelsa.no
Gaarder
P I
PI
Groeng
E
E
Leikvold
R
R
Namork
E
E
Lovik
M
M
D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology*; D000485:Allergens / Q000737:chemistry / Q000276:immunology* / Q000494:pharmacology; D000818:Animals; D000918:Antibody Specificity; D002645:Chickens; D005260:Female; D007073:Immunoglobulin E / Q000096:biosynthesis* / Q000097:blood / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000097:blood / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D017830:Octoxynol / Q000737:chemistry / Q000494:pharmacology; D010047:Ovalbumin / Q000276:immunology; D010316:Particle Size; D011137:Polystyrenes / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology; D011138:Polytetrafluoroethylene / Q000737:chemistry / Q000494:pharmacology; D012822:Silicon Dioxide / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology; D014025:Titanium / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology
D000276:Adjuvants, Immunologic; D000485:Allergens; D007074:Immunoglobulin G; D011137:Polystyrenes; D013501:Surface-Active Agents; C009495:titanium dioxide; D007073:Immunoglobulin E; D012822:Silicon Dioxide; D011138:Polytetrafluoroethylene; D017830:Octoxynol; D010047:Ovalbumin; D014025:Titanium