title: Isolation and characterization of the cell-surface polysaccharides of Porphyromonas gingivalis ATCC 53978.
abstract: The cell-surface carbohydrates of Porphyromonas gingivalis strain ATCC 53978 were isolated and partially characterized. Three separate polysaccharides were found to be present: an extracellular polysaccharide, capsular polysaccharide and lipopolysaccharide. The capsular polysaccharide, which had peculiar, gel-like viscoelastic properties, was found to be comprised of mannuronic acid, glucuronic acid, galacturonic acid, galactose, and 2-acetamido-2-deoxy-D-glucose in relative molar ratios of 0.6:0.9:0.5:0.5:1.0, respectively. The extracellular polysaccharide was found to contain mannose, rhamnose, glucose, galactose, and 2-acetamido-2-deoxy-D-glucose in relative molar ratios of 13.5:1.4:1.0:2.0:1.0, respectively. The lipopolysaccharide was found to contain an O-antigen with a regular tetrasaccharide repeat unit comprised of 4-linked alpha-L-rhamnopyranosyl, 6-linked alpha-D-glucopyranosyl, 3-linked alpha-D-galactopyranosyl, and 4-linked 2-acetamido-2-deoxy-beta-D-glucopyranosyl residues in equimolar proportions.
authors.affiliation: Department of Chemistry, University of Minnesota, 207 Pleasant St. SE, Minneapolis, MN 55455, USA.
authors.forename: S I
authors.initials: SI
authors.lastname: Farquharson
authors.forename: G R
authors.initials: GR
authors.lastname: Germaine
authors.forename: G R
authors.initials: GR
authors.lastname: Gray
mesh_terms: D002849:Chromatography, Gas; D009682:Magnetic Resonance Spectroscopy; D009994:Osmolar Concentration; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification*; D016966:Porphyromonas gingivalis / Q000737:chemistry*
chemical_list: D011135:Polysaccharides, Bacterial