Molecular cloning and functional analysis of mouse C-terminal kinesin motor KifC3.
Proteins of the kinesin superfamily define a class of microtubule-dependent motors that play crucial roles in cell division and intracellular transport. To study the molecular mechanism of intracellular transport involving microtubule-dependent motors, a cDNA encoding a new kinesin-like protein called KifC3 was cloned from a mouse brain cDNA library. Sequence and secondary structure analysis revealed that KifC3 is a member of the C-terminal motor family. In contrast to other mouse C-terminal motors, KifC3 is apparently ubiquitous and may have a general role in intracellular transport. To understand the in vivo function of the KifC3 gene, we used homologous recombination in embryonic stem cells to construct knockout mouse strains for the KifC3 gene. Homozygous mutants of the KifC3 gene are viable, reproduce normally, and apparently develop normally. These results suggest that KifC3 is dispensable for normal development and reproduction in the mouse.
Yang
Z
Z
Howard Hughes Medical Institute, Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, California 92093, USA.
Xia
Ch
Ch
Roberts
E A
EA
Bush
K
K
Nigam
S K
SK
Goldstein
L S
LS
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006128:Growth / Q000235:genetics / Q000502:physiology; D007668:Kidney / Q000033:anatomy & histology / Q000378:metabolism; D016547:Kinesins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D020409:Molecular Motor Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D008969:Molecular Sequence Data; D010641:Phenotype; D011247:Pregnancy; D012098:Reproduction / Q000235:genetics / Q000502:physiology; D012160:Retina / Q000378:metabolism
D018076:DNA, Complementary; C419428:Kifc3 protein, mouse; D020409:Molecular Motor Proteins; D016547:Kinesins