An intronless gene encoding a poly(A) polymerase is specifically expressed in testis.
Previous work demonstrated that a single pre-mRNA could generate multiple forms of mammalian poly(A) polymerase mRNAs by alternative splicing or alternative polyadenylation. A cDNA encoding a testis-specific poly(A) polymerase was isolated in this study. The transcription level of Papt in testis of a 2 weeks old mouse was much lower than that of the general poly(A) polymerase gene, Pap. However, the transcription ratio of Papt to Pap was reversed in testis of a 4 weeks old mouse. Transient expression analysis showed that GFP-Papt fusion protein is present both in the nucleus and cytoplasm of HeLa cells. These results suggest that Papt is involved in polyadenylation of transcripts expressed during spermatogenesis.
Lee
Y J
YJ
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejon, South Korea.
Lee
Y
Y
Chung
J H
JH
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology; D003593:Cytoplasm / Q000201:enzymology; D015723:Gene Library; D049452:Green Fluorescent Proteins; D006367:HeLa Cells; D006801:Humans; D007438:Introns; D008164:Luminescent Proteins / Q000032:analysis / Q000235:genetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011062:Polynucleotide Adenylyltransferase / Q000032:analysis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000032:analysis; D015183:Restriction Mapping; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013737:Testis / Q000201:enzymology*; D014158:Transcription, Genetic; D014162:Transfection
D008164:Luminescent Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D011062:Polynucleotide Adenylyltransferase