Characterization of monoclonal antibodies against mouse and rat platelet glycoprotein V (CD42d).
The mouse- and rat-platelet-specific hamster monoclonal antibody (MAb) 1C2, previously found to react with a thrombin-sensitive 74-kD glycoprotein, was now shown to recognize platelet glycoprotein V (GPV, CD42d). 1C2 reacted with NIH-3T3 cells in which recombinant mouse or rat GPV was expressed. Both 1C2 and 4A5, another mouse-platelet-specific rat MAb, immunoprecipitated GVP, although they recognized different epitopes. Side-by-side comparison confirmed that 1C2 as well as RPM.9, a MAb against rat GPV, recognized the same rat platelet molecule. In a mouse bone marrow culture, 1C2+ megakaryocytes emerged from CD41 (GPIIb)+1C2- megakaryocytes. Because 1C2+ megakaryocytes exhibited higher DNA ploidy distribution than CD41+ cells, GPV likely appears in the late stage of megakaryocyte maturation. This study established 1C2 as a MAb against mouse and rat GPV, namely CD42d, and as useful tool to study rodent megakaryopoiesis.
Sato
N
N
Department of Pathology, National Children's Medical Research Center, Tokyo, Japan.
Kiyokawa
N
N
Takada
K
K
Itagaki
M
M
Saito
M
M
Sekino
T
T
Suzuki
T
T
Taguchi
T
T
Mimori
K
K
Lanza
F
F
Fujimoto
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D000918:Antibody Specificity; D015415:Biomarkers / Q000032:analysis; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000276:immunology; D006224:Cricetinae; D018604:Epitope Mapping; D008533:Megakaryocytes / Q000166:cytology / Q000276:immunology; D051379:Mice; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000235:genetics / Q000276:immunology*; D051381:Rats
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D019038:Platelet Glycoprotein GPIb-IX Complex