Use of optical biosensors for the study of mechanistically concerted surface adsorption processes.
The advent of commercial optical biosensors, such as the BIAcore from Pharmacia and IAsys from Affinity Sensors, has made available to the biochemist a powerful means to examine and characterize the interaction of biological macromolecules with a binding surface. By analysis of the kinetic and equilibrium aspects of the observed experimental adsorption isotherms, rate and affinity constants can be determined. This Review focuses on pertinent aspects of the technology and its use for the performance and quantitative characterization of some various types of mechanistically concerted adsorption behavior.
Hall
D
D
Section on Physical Biochemistry, National Institute of Diabetes, Digestive, and Kidney Disease, Bethesda, Maryland, 20892, USA. dhall@helix.nih.gov
D000327:Adsorption; D015374:Biosensing Techniques; D007700:Kinetics; D055095:Optics and Photonics