Protective effect of resveratrol on oxidative damage in male and female stroke-prone spontaneously hypertensive rats.
1. In the present study, we examined the effect of resveratrol (3,4',5-trihydroxystilbene), a phytoestrogen found in the skins of most grapes, on oxidative DNA damage in male and female stroke-prone spontaneously hypertensive rats (SHRSP). 2. Five-week-old male and female SHRSP were divided into control and resveratrol groups. The resveratrol group was given 1 mg/kg per day, orally, resveratrol by gastric intubation once a day. 3. Following an 8 week feeding period, the levels of 8-hydroxydeoxyguanosine (8-OHdG), produced from deoxyguanosine under conditions of oxidative stress, in the urine of male and female resveratrol-treated SHRSP were significantly lower than that in control SHRSP. 4. The urine of resveratrol-treated male and female SHRSP had lower levels of hydroperoxide compared with control SHRSP, but the difference was not significant. 5. Treatment with resveratrol resulted in a 25 and 30% reduction in plasma glycated albumin in male and female SHRSP, respectively, compared with controls. 6. Gender differences for SHRSP with regard to 8-OHdG, hydroperoxide and glycated albumin levels were not confirmed, resveratrol having similar protective effects on male and female SHRSP. 7. These results indicate that dietary resveratrol: (i) plays a role in suppressing oxidative DNA damage and glycoxidative stress in vivo; and (ii) has similar protective effects in both male and female SHRSP, suggesting that the direct effects of this phytoestrogen on oxidative stress in vivo are not sexually dimorphic.
Mizutani
K
K
Life Science, Environmental Conservation and Development, Graduate School of Human and Environmental Studies, Kyoto University, Japan. mizutani@fischer.jinkan.kyoto-u.ac.jp
Ikeda
K
K
Kawai
Y
Y
Yamori
Y
Y
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001774:Blood Chemical Analysis; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology; D001835:Body Weight / Q000187:drug effects / Q000502:physiology; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine*; D005260:Female; D017127:Glycation End Products, Advanced; D006861:Hydrogen Peroxide / Q000652:urine*; D008297:Male; D009929:Organ Size / Q000187:drug effects / Q000502:physiology; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D011918:Rats, Inbred SHR; D000077185:Resveratrol; D012709:Serum Albumin; D013267:Stilbenes / Q000494:pharmacology*; D000093423:Glycated Serum Albumin
D000975:Antioxidants; D017127:Glycation End Products, Advanced; D012709:Serum Albumin; D013267:Stilbenes; D000080242:8-Hydroxy-2'-Deoxyguanosine; D006861:Hydrogen Peroxide; D003849:Deoxyguanosine; D000077185:Resveratrol; D000093423:Glycated Serum Albumin