Evidence for expression and function of phosphodiesterase type 5 (PDE-V) in rat resistance arteries.
Evidence is provided for expression and a functional role for phosphodiesterase type V (PDE-V) in the rat isolated small mesenteric artery. The reverse transcription polymerase chain reaction (RT--PCR) demonstrated mRNA for PDE-V, while Western blotting and immunocytochemical studies showed corresponding protein expression. Smooth muscle relaxation to the nitric oxide donor, diethylamine NONOate (DEA NONOate; 1 nM - 10 microM; pEC(50)=6.7+/-0.3) was potentiated significantly by the specific inhibitor of PDE-V, 4-[[3,4-(methylenedioxy)benzyl]amino]-6-chloroquinazoline (MBCQ; 1 microM; pEC(50)=10.5+/-0.04). These data show that PDE-V is expressed in both the smooth muscle and endothelial cells of a resistance artery, and the enzyme can significantly influence nitric oxide-evoked vasorelaxation.
Sampson
L J
LJ
Cardiovascular Research Laboratories, School of Medical Sciences, University of Bristol, University Walk, Bristol, BS8 1TD.
Hinton
J M
JM
Garland
C J
CJ
D015106:3',5'-Cyclic-GMP Phosphodiesterases; D000818:Animals; D001158:Arteries / Q000187:drug effects / Q000201:enzymology* / Q000502:physiology*; D015153:Blotting, Western; D054706:Cyclic Nucleotide Phosphodiesterases, Type 5; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008297:Male; D008638:Mesenteric Arteries / Q000187:drug effects / Q000502:physiology; D009126:Muscle Relaxation / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D020030:Nitric Oxide Donors / Q000494:pharmacology; D010727:Phosphoric Diester Hydrolases / Q000096:biosynthesis* / Q000502:physiology*; D011233:Precipitin Tests; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014655:Vascular Resistance / Q000187:drug effects / Q000502:physiology*
D020030:Nitric Oxide Donors; D012333:RNA, Messenger; D010727:Phosphoric Diester Hydrolases; D015106:3',5'-Cyclic-GMP Phosphodiesterases; D054706:Cyclic Nucleotide Phosphodiesterases, Type 5; C517384:Pde5a protein, rat