title: Rapid hypothermic aortic flush can achieve survival without brain damage after 30 minutes cardiac arrest in dogs.
abstract: BACKGROUND
  Neither exsanguination to pulselessness nor cardiac arrest of 30 min duration can be reversed with complete neurologic recovery using conventional resuscitation methods. Techniques that might buy time for transport, surgical hemostasis, and initiation of cardiopulmonary bypass or other resuscitation methods would be valuable. We hypothesized that an aortic flush with high-volume cold normal saline solution at the start of exsanguination cardiac arrest could rapidly preserve cerebral viability during 30 min of complete global ischemia and achieve good outcome.
  
  
  METHODS
  Sixteen dogs weighing 20-25 kg were exsanguinated to pulselessness over 5 min, and circulatory arrest was maintained for another 30 min. They were then resuscitated using closed-chest cardiopulmonary bypass and had assisted circulation for 2 h, mild hypothermia (34 degrees C) for 12 h, controlled ventilation for 20 h, and intensive care to outcome evaluation at 72 h. Two minutes after the onset of circulatory arrest, the dogs received a flush of normal saline solution at 4 degrees C into the aorta (cephalad) via a balloon catheter. Group I (n = 6) received a flush of 25 ml/kg saline with the balloon in the thoracic aorta; group II (n = 7) received a flush of 100 ml/kg saline with the balloon in the abdominal aorta.
  
  
  RESULTS
  The aortic flush decreased mean tympanic membrane temperature (Tty) in group I from 37.6 +/- 0.1 to 33.3 +/- 1.6 degrees C and in group II from 37.5 +/- 0.1 to 28.3 +/- 2.4 degrees C (P = 0.001). In group 1, four dogs achieved overall performance category (OPC) 4 (coma), and 2 dogs achieved OPC 5 (brain death). In group II, 4 dogs achieved OPC 1 (normal), and 3 dogs achieved OPC 2 (moderate disability). Median (interquartile range [IQR]) neurologic deficit scores (NDS 0-10% = normal; NDS 100% = brain death) were 69% (56-99%) in group I versus 4% (0-15%) in group II (P = 0.003). Median total brain histologic damage scores (HDS 0 = no damage; > 100 = extensive damage; 1,064 = maximal damage) were 144 (74-168) in group I versus 18 (3-36) in group II (P = 0.004); in three dogs from group II, the brain was histologically normal (HDS 0-5).
  
  
  CONCLUSIONS
  A single high-volume flush of cold saline (4 degrees C) into the abdominal aorta given 2 min after the onset of cardiac arrest rapidly induces moderate-to-deep cerebral hypothermia and can result in survival without functional or histologic brain damage, even after 30 min of no blood flow.
authors.affiliation: Safar Center for Resuscitation Research of the University of Pittsburgh, Pennsylvania 15260, USA.
authors.forename: W
authors.initials: W
authors.lastname: Behringer
authors.forename: S
authors.initials: S
authors.lastname: Prueckner
authors.forename: R
authors.initials: R
authors.lastname: Kentner
authors.forename: S A
authors.initials: SA
authors.lastname: Tisherman
authors.forename: A
authors.initials: A
authors.lastname: Radovsky
authors.forename: R
authors.initials: R
authors.lastname: Clark
authors.forename: S W
authors.initials: SW
authors.lastname: Stezoski
authors.forename: J
authors.initials: J
authors.lastname: Henchir
authors.forename: E
authors.initials: E
authors.lastname: Klein
authors.forename: P
authors.initials: P
authors.lastname: Safar
mesh_terms: D000818:Animals; D001012:Aorta, Abdominal; D001831:Body Temperature; D001926:Brain Death; D002545:Brain Ischemia / Q000517:prevention & control*; D004285:Dogs; D006324:Heart Arrest, Induced / Q000379:methods* / Q000401:mortality; D007036:Hypothermia, Induced / Q000379:methods* / Q000401:mortality; D007261:Infusions, Intra-Arterial / Q000379:methods*; D008297:Male; D009460:Neurologic Examination; D012965:Sodium Chloride / Q000008:administration & dosage*; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D012965:Sodium Chloride