title: Comparison of five cyclosporin immunoassays with HPLC.
abstract: High performance liquid chromatography (HPLC) is the reference method for cyclosporin (CyA) measurements but therapeutic monitoring of the drug is frequently made using the more practical immunoassays. Cross-reactivity with CyA metabolites may compromise the specificity of immunoassays, particularly in liver graft recipients where metabolites may accumulate. The aim of this study was to compare with HPLC the performance of two recently introduced CyA immunoassays (the AxSYM fluorescent polarisation immunoassay (FPIA) and non-extraction CEDIA assay). The comparison was extended to the well-established TDx monoclonal FPIA (TDx mono) and the enzyme multiplied (EMIT)-specific assays and to the polyclonal FPIA (TDx poly), in which metabolite cross-reactivity is extensive. Assays were performed on 106 blood samples (taken 6 days to 118 months post-liver transplant) and results were compared by non-parametric regression analysis and difference plots. AxSYM and CEDIA showed both constant and proportional bias against HPLC (unlike EMIT) but the mean difference from HPLC was least for AxSYM (2.7 microg/l vs. 11.7, 9.4 and 54 microg/l for CEDIA, EMIT and TDx mono, respectively. (TDx poly - HPLC) values were proportional to all immunoassay results, with slopes of 0.33, 0.38 and 0.45 for EMIT, AxSYM and CEDIA, respectively. Our data suggest close agreement between AxSYM, CEDIA and EMIT results.
authors.affiliation: Institute of Liver Studies, King's College Hospital, London, UK. michael.tredger@kcl.ac.uk
authors.forename: J M
authors.initials: JM
authors.lastname: Tredger
authors.forename: N
authors.initials: N
authors.lastname: Roberts
authors.forename: R
authors.initials: R
authors.lastname: Sherwood
authors.forename: G
authors.initials: G
authors.lastname: Higgins
authors.forename: J
authors.initials: J
authors.lastname: Keating
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003524:Cyclosporins / Q000032:analysis*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007166:Immunosuppressive Agents / Q000032:analysis*; D016031:Liver Transplantation
chemical_list: D003524:Cyclosporins; D007166:Immunosuppressive Agents