title: Unexpected presence of defective glpR alleles in various strains of Escherichia coli.
abstract: Alleles of glpR associated with the same GlpR(-) phenotype produce substitutions in different conserved portions of the glycerol 3-phosphate repressor which are not part of the helix-turn-helix motif. Analysis of the effects on growth and enzyme expression show that glucose repression of glycerol utilization is not dependent on a functional repressor.
authors.affiliation: Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas 77843-2128, USA.
authors.forename: C K
authors.initials: CK
authors.lastname: Holtman
authors.forename: R
authors.initials: R
authors.lastname: Thurlkill
authors.forename: D W
authors.initials: DW
authors.lastname: Pettigrew
mesh_terms: D000483:Alleles; D001426:Bacterial Proteins; D004794:Enzyme Repression; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D005947:Glucose / Q000494:pharmacology; D005990:Glycerol / Q000378:metabolism; D005991:Glycerol Kinase / Q000096:biosynthesis*; D012097:Repressor Proteins / Q000235:genetics*; D013045:Species Specificity; D014157:Transcription Factors
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C101651:GlpR protein, E coli; D012097:Repressor Proteins; D014157:Transcription Factors; D005991:Glycerol Kinase; D005947:Glucose; D005990:Glycerol