title: Electrooxidation mechanism of biogenic amines at amine oxidase modified graphite electrode.
abstract: Amine oxidase (AO, EC. 1.4.3.6) was previously shown to be a very efficient biological recognition element of amperometric biosensors for monitoring biogenic amines. The enzyme was effectively working in both mono- and bienzyme electrode designs, based on either a direct or a mediated electron-transfer pathway. This work focuses on the elucidation of the electron-transfer mechanism of the monoenzymatic unmediated AO-modified biosensor. The observed unmediated catalytic currents were assumed to be caused by (i) a direct electron-transfer process, (ii) the electrooxidation of the formed product, or (iii) their combination. Experiments supporting these assumptions are discussed in detail.
authors.affiliation: Department of Biotechnology, Lund University, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Niculescu
authors.forename: T
authors.initials: T
authors.lastname: Ruzgas
authors.forename: C
authors.initials: C
authors.lastname: Nistor
authors.forename: I
authors.initials: I
authors.lastname: Frébort
authors.forename: M
authors.initials: M
authors.lastname: Sebela
authors.forename: P
authors.initials: P
authors.lastname: Pec
authors.forename: E
authors.initials: E
authors.lastname: Csöregi
mesh_terms: D006631:Amine Oxidase (Copper-Containing) / Q000737:chemistry / Q000378:metabolism*; D015306:Biogenic Monoamines / Q000737:chemistry / Q000378:metabolism*; D002384:Catalysis; D004566:Electrodes; D010084:Oxidation-Reduction
chemical_list: D015306:Biogenic Monoamines; D006631:Amine Oxidase (Copper-Containing)