title: Clonal accumulation of T cells bearing V beta 6 T-cell receptor in chronic inflammatory periodontal disease.
abstract: Our previous study demonstrated that among the various T-cell receptor V beta gene segments, T-cell receptor V beta 6 gene-bearing T cells are predominant in periodontitis lesions. To explore the clonality of T cells expressing V beta 6 T-cell receptor, which is determined by the heterogeneity of the complementarity-determining region 3(CDR3), V beta 6-C beta polymerase chain reaction products derived from gingival tissue and peripheral blood of periodontitis patients were analyzed by single-strand conformation polymorphism and nucleotide sequence. Both techniques provided quite similar results in terms of the number of accumulated clones in which clonal accumulation of T-cell receptor V beta 6-bearing T cells was found in most periodontitis lesions but not in peripheral blood. From the analysis of deduced amino acid sequences, a possible conserved amino acid residue in the CDR3 region of the periodontitis lesion-derived sequences was found in most patients. This conserved amino acid motif however, was not identified. Furthermore, skewed J beta gene segment usage was observed for expanded clones from periodontitis lesions. Our results suggest that T cells infiltrating periodontitis lesions are responding to a restricted number of antigens or epitopes.
authors.affiliation: Department of Periodontology, Faculty of Dentistry, Niigata University, Niigata, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Ohsawa
authors.forename: K
authors.initials: K
authors.lastname: Yamazaki
authors.forename: T
authors.initials: T
authors.lastname: Nakajima
authors.forename: K
authors.initials: K
authors.lastname: Hara
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D001483:Base Sequence; D002908:Chronic Disease; D002999:Clone Cells; D022801:Complementarity Determining Regions / Q000235:genetics; D017124:Conserved Sequence; D019674:Genes, T-Cell Receptor beta; D006801:Humans; D008875:Middle Aged; D008969:Molecular Sequence Data; D010518:Periodontitis / Q000276:immunology*; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000737:chemistry / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D022801:Complementarity Determining Regions; D016693:Receptors, Antigen, T-Cell, alpha-beta