[Surveillance of group illnesses with Norwalk-like viruses (NLV) in Baden-Württemberg. Isolation and detection of Norwalk-like viral RNA from fecal samples with RT-PCR].
Between the turn of the years 98/99 and 99/2000, stool samples from various health departments in the south of Germany were sent to the LGA (Landesgesundheitsamt Baden-Württemberg). Different testings as bacteriological routines for enterobacteriaceae and virological antigen screenings of the stool samples for adeno-, astro- and rotaviruses lead to negative results. Additional RT-PCR tests for the presence of Norwalk-RNA showed nearly 40% of positive samples. From 14 group infections between december 98 and january 2000, 286 samples in total were sent to the LGA, 118 of them gave positive results for the norwalk agent. For the verification of the analysis, some random samples were sent to the Robert-Koch-Institut, div. of molecular virology at Berlin, where the results were confirmed.
Fleischer
J
J
Landesgesundheitsamt Baden-Württemberg.
Wagner Wiening
C
C
Kimmig
P
P
D017250:Caliciviridae Infections / Q000821:virology*; D004196:Disease Outbreaks; D005243:Feces / Q000821:virology*; D005500:Follow-Up Studies; D005759:Gastroenteritis / Q000821:virology*; D005858:Germany; D006801:Humans; D009663:Norwalk virus / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012367:RNA, Viral