Long-term administration of highly purified eicosapentaenoic acid ethyl ester improves the dysfunction of vascular endothelial and smooth muscle cells in male WBN/Kob rats.
We investigated the effect of long-term administration of highly purified eicosapentaenoic acid ethyl ester (EPA-E), an n-3 polyunsaturated fatty acid, on the dysfunction of the endothelium and smooth muscle cells in male WBN/Kob rats, a model of spontaneous diabetes mellitus. After oral 8-month treatment with EPA-E, the agent significantly and dose-dependently increased the migration activity of vascular endothelial cells and also decreased 5-bromodeoxyuridine (BrdU) uptake by vascular smooth muscle cells at a dose of 0.1 g/kg or higher. In addition, there were significant correlations between the endothelial cell migration or smooth muscle cell proliferation and the 4-hour fasting glucose level. These findings suggest that EPA-E has a suppressive effect on thrombosis and atherosclerosis.
Nobukata
H
H
Toxicology Laboratory, Research Center, Mochida Pharmaceutical, Fujieda, Shizuoka, Japan.
Ishikawa
T
T
Obata
M
M
Shibutani
Y
Y
D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D003920:Diabetes Mellitus / Q000473:pathology / Q000503:physiopathology*; D004305:Dose-Response Relationship, Drug; D015118:Eicosapentaenoic Acid / Q000008:administration & dosage* / Q000031:analogs & derivatives* / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology*; D005951:Glucose Tolerance Test; D017997:Leukotriene C4 / Q000494:pharmacology; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology*; D051381:Rats; D011919:Rats, Inbred Strains; D017208:Rats, Wistar; D013997:Time Factors
D017997:Leukotriene C4; C035276:eicosapentaenoic acid ethyl ester; D015118:Eicosapentaenoic Acid; D001973:Bromodeoxyuridine