title: Immunodiagnosis of human fascioliasis by an enzyme-linked immunosorbent assay (ELISA) and a micro-ELISA.
abstract: Enzyme-linked immunosorbent assay (ELISA) and micro-ELISA were evaluated for their ability to detect anti-Fasciola hepatica antibodies in humans by using excretory-secretory antigen. The sensitivity of each method was 100%, but the specificity was 100% for ELISA and 97% for micro-ELISA. The micro-ELISA could be used as a screening assay and ELISA could be used as a confirmatory method for the serodiagnosis of human fascioliasis.
authors.affiliation: Departamento de Parasitología Sanitaria, Instituto Nacional de Parasitología, ANLIS "Dr. Carlos G. Malbrán," 1281 Buenos Aires, Argentina.
authors.forename: S
authors.initials: S
authors.lastname: Carnevale
authors.forename: M I
authors.initials: MI
authors.lastname: Rodríguez
authors.forename: G
authors.initials: G
authors.lastname: Santillán
authors.forename: J H
authors.initials: JH
authors.lastname: Labbé
authors.forename: M G
authors.initials: MG
authors.lastname: Cabrera
authors.forename: E J
authors.initials: EJ
authors.lastname: Bellegarde
authors.forename: J N
authors.initials: JN
authors.lastname: Velásquez
authors.forename: J E
authors.initials: JE
authors.lastname: Trgovcic
authors.forename: E A
authors.initials: EA
authors.lastname: Guarnera
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood* / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005211:Fascioliasis / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D006801:Humans; D007159:Immunologic Tests / Q000379:methods
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth