title: [D-Leu1]Microcystin-LR, from the cyanobacterium Microcystis RST 9501 and from a Microcystis bloom in the Patos Lagoon estuary, Brazil.
abstract: [D-Leu1]Microcystin-LR was identified as the most abundant microcystin from a laboratory strain of the cyanobacterium Microcystis sp. isolated from a hepatotoxic Microcystis bloom from brackish waters in the Patos Lagoon estuary, southern Brazil. Toxicity of [D-Leu1]microcystin-LR, according to bioassay and protein phosphatase inhibition assay, was similar to that of the commonly-occurring microcystin-LR, which was not detectable in the Patos Lagoon laboratory isolate. This is the first report of a microcystin containing [D-Leu1] in the cyclic heptapeptide structure of these potent cyanobacterial toxins.
authors.affiliation: Department of Biological Sciences, University of Dundee, UK.
authors.forename: A
authors.initials: A
authors.lastname: Matthiensen
authors.forename: K A
authors.initials: KA
authors.lastname: Beattie
authors.forename: J S
authors.initials: JS
authors.lastname: Yunes
authors.forename: K
authors.initials: K
authors.lastname: Kaya
authors.forename: G A
authors.initials: GA
authors.lastname: Codd
mesh_terms: D001938:Brazil; D009682:Magnetic Resonance Spectroscopy; D052998:Microcystins; D046931:Microcystis / Q000737:chemistry* / Q000254:growth & development; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification*; D016339:Spectrometry, Mass, Fast Atom Bombardment
chemical_list: C420867:Leu(1)microcystin-LR; D052998:Microcystins; D010456:Peptides, Cyclic