Peptide-based targeting of fluorophores to peroxisomes in living cells.
Peptides carrying different fluorophores can be designed to incorporate spontaneously into living cells when added to the medium. By incorporating the peroxisome-targeting sequence PTS1, the peptide is recognized by the protein-import machinery of peroxisomes and, as a result, can accumulate in these organelles. Depending on the cell type, an inhibitor of the multidrug-resistance protein might be required to ensure strong accumulation. In this update, we discuss the potential of these peptide-linked fluorophores in solving issues related to organelle function and dynamics.
Pap
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EH
Biochemistry of Lipids Dept, Utrecht University, PO Box 80.054, 3508 TB, Utrecht, The Netherlands.
Dansen
T B
TB
Wirtz
K W
KW
D000818:Animals; D002463:Cell Membrane Permeability; D018432:Drug Resistance, Multiple; D005456:Fluorescent Dyes / Q000378:metabolism*; D005544:Forecasting; D010455:Peptides / Q000378:metabolism*; D000074435:Peroxisome-Targeting Signal 1 Receptor; D020675:Peroxisomes / Q000378:metabolism*; D021382:Protein Sorting Signals; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*
D005456:Fluorescent Dyes; D010455:Peptides; D000074435:Peroxisome-Targeting Signal 1 Receptor; D021382:Protein Sorting Signals; D018160:Receptors, Cytoplasmic and Nuclear