Efficacy of pertussis vaccines consisted of antigens detoxified with tea-leaf catechins.
The ability of tea-leaf catechins to detoxify agents was examined. Filamentous hemagglutinin (FHA) and pertussis toxin (PT) were detoxified by the catechins at an extraordinarily lower concentration compared with that of formalin. The sera from the mice immunized by the catechin-treated antigens recognized, not only catechin-treated, but also untreated antigens. Furthermore, catechin-treated PT induced the antibody to neutralize PT activity in the sera of the immunized mice. Pertussis vaccines were prepared including antigens detoxified by the treatment of catechins and intraperitoneally injected into mice. Protection against Bordetella pertussis infection was shown in mice immunized with the vaccines prepared by treatment with catechins. These data suggest that catechins are effective toxoiding agents for preparing a pertussis vaccine.
Watanabe
M
M
Department of Microbiology and Biologics, Daiichi College of Pharmaceutical Sciences, 22-1 Tamagawa-cho, Minami-ku, 815-8511, Fukuoka, Japan. min-ind@umin.ac.jp
Funaishi
K
K
Takeo
T
T
Endoh
M
M
D018829:Adhesins, Bacterial / Q000187:drug effects / Q000276:immunology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000942:Antigens, Bacterial / Q000276:immunology*; D002392:Catechin / Q000494:pharmacology*; D005260:Female; D006388:Hemagglutinins / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D037342:Pertussis Toxin; D010567:Pertussis Vaccine / Q000276:immunology*; D013662:Tea; D022282:Vaccines, Acellular / Q000276:immunology; D010566:Virulence Factors, Bordetella / Q000276:immunology*
D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D006388:Hemagglutinins; D010567:Pertussis Vaccine; D013662:Tea; D022282:Vaccines, Acellular; D010566:Virulence Factors, Bordetella; C065273:filamentous hemagglutinin adhesin, Bordetella pertussis; D002392:Catechin; D037342:Pertussis Toxin