Intestinal transit of an orally administered streptomycin-rifampicin-resistant variant of Bifidobacterium longum SBT2928: its long-term survival and effect on the intestinal microflora and metabolism.
OBJECTIVE
The objectives of this study are to investigate the fate of a streptomycin-rifampicin-resistant variant of Bifidobacterium longum SBT2928 (BL2928SR) and the influence of its oral administration on the composition and metabolism of the intestinal microflora.


RESULTS
Intestinal passage of BL2928SR was monitored by a combination of selection with antibiotics and identification by a randomly amplified polymorphic DNA (RAPD)-PCR method. Intestinal microflora was analysed by the method developed by Mitsuoka et al. (1965, 1974). Long-term survival of orally administered BL2928SR in the human intestine was confirmed. BL2928SR ingestion specifically lowered faecal populations of Enterobacteriaceae and clostridia, including lecithinase-positive Clostridium spp.


CONCLUSIONS
BL2928SR and its parent strain, BL2928, are considered to be appropriate candidates for probiotics.


CONCLUSIONS
It is clarified that BL2928SR has the ability for long-term survival in the human gastrointestinal tract, and alters the composition and metabolism of the intestinal microflora.
Fujiwara
S
S
Technology and Research Institute, Snow Band Milk Products Co. Ltd, Kawagoe, Saitama, Japan. ht4s-fjwr@asahi-net.or.jp
Seto
Y
Y
Kimura
A
A
Hashiba
H
H
D000193:Actinomycetales Infections / Q000201:enzymology / Q000378:metabolism / Q000382:microbiology; D000284:Administration, Oral; D000328:Adult; D000641:Ammonia / Q000032:analysis; D001419:Bacteria / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D001644:Bifidobacterium / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D002455:Cell Division; D004352:Drug Resistance, Microbial; D018432:Drug Resistance, Multiple; D005243:Feces / Q000737:chemistry / Q000201:enzymology / Q000382:microbiology*; D005260:Female; D014644:Genetic Variation / Q000235:genetics; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000737:chemistry / Q000201:enzymology / Q000382:microbiology*; D008297:Male; D009154:Mutation / Q000235:genetics; D019936:Probiotics / Q000008:administration & dosage / Q000378:metabolism; D019105:Random Amplified Polymorphic DNA Technique; D011795:Surveys and Questionnaires
D000641:Ammonia