title: Gentamicin release from polymethylmethacrylate bone cements and Staphylococcus aureus biofilm formation.
abstract: We measured the formation of a Staphylococcus aureus biofilm in vitro on unloaded and gentamicin-loaded bone cements (CMW3 and Palacos R) and related the formation to antibiotic release rates. All experiments were done in triplicate. Microbial growth on gentamicin-loaded cements occurred despite the release of antibiotic. Biofilm formation on gentamicin loaded CMW3 bone cement was one fourth to one fifth less than on the unloaded bone cement, while biofilm formation on Palacos R bone cement was not significantly affected by antibiotic loading. More gentamicin was released from CMW3 (79 mg) than from Palacos R (70 mg), but the percentage gentamicin released after one week relative to the total amount incorporated was significantly lower for CMW3 (4.7%) than for Palacos R (8.4%). After one day, subinhibitory concentrations of antibiotics were eluted from the cements. We concluded that antibiotic-loaded bone cement does not necessarily inhibit the formation of an infectious biofilm in vitro.
authors.affiliation: Department of Orthopedic Surgery, University Hospital Groningen, The Netherlands.
authors.forename: H
authors.initials: H
authors.lastname: van de Belt
authors.forename: D
authors.initials: D
authors.lastname: Neut
authors.forename: W
authors.initials: W
authors.lastname: Schenk
authors.forename: J R
authors.initials: JR
authors.lastname: van Horn
authors.forename: H C
authors.initials: HC
authors.lastname: van der Mei
authors.forename: H J
authors.initials: HJ
authors.lastname: Busscher
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics; D018441:Biofilms; D001843:Bone Cements; D016503:Drug Delivery Systems; D005839:Gentamicins / Q000008:administration & dosage* / Q000493:pharmacokinetics; D066298:In Vitro Techniques; D019904:Polymethyl Methacrylate; D016459:Prosthesis-Related Infections / Q000517:prevention & control; D013203:Staphylococcal Infections / Q000517:prevention & control; D013211:Staphylococcus aureus
chemical_list: D000900:Anti-Bacterial Agents; D001843:Bone Cements; D005839:Gentamicins; D019904:Polymethyl Methacrylate