Carnosine protects rats under global ischemia.
Rat brain subjected to 45-min global ischemia is characterized by decreased activity of K-p-nitrophenyl phosphatase and monoamine oxidase B and a disordering of the membrane bilayer by reactive oxygen species attack, the latter being monitored by the fluorescence of the membrane fluorescent probe, 1-anilino, 8-naphtalene sulphonate (ANS). Ischemic injury resulted in 67% mortality of the animals. In the group of animals pre-treated with the neuropeptide carnosine the mortality was only 30%. At the same time, carnosine protected both the activity of the above-mentioned enzymes and the brain membrane disordering, which was also tested by ANS fluorescence. The conclusion was made that carnosine protects the brain against oxidative injury and thereby increases the survival of the animals.
Stvolinsky
S
S
Laboratory of Neurochemistry, Institute of Neurology, Russian Academy of Medical Sciences, Moscow, Russia.
Kukley
M
M
Dobrota
D
D
Mezesova
V
V
Boldyrev
A
A
D009597:4-Nitrophenylphosphatase / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000201:enzymology / Q000473:pathology; D002336:Carnosine / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects / Q000473:pathology; D002546:Ischemic Attack, Transient / Q000201:enzymology* / Q000517:prevention & control*; D008297:Male; D008995:Monoamine Oxidase / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D002336:Carnosine; D008995:Monoamine Oxidase; D009597:4-Nitrophenylphosphatase