title: Preparation of 99mTc-ethylene dicysteine (99mTc-EC) by transchelation using 99mTc-glucoheptonate (99mTc-GHA) and its evaluation for renal function studies.
abstract: The complexation of ethylene dicysteine (EC) with 99mTc needs to be carried out at pH 12 to achieve a high radiochemical yield. However, the preparation of the kit at high pH poses difficulties and requires very stringent preparation conditions, as stannous tin, one of the main ingredients in the kit, is unstable at high pH. Hence, an alternative method, involving the transchelation preparation of 99mTc-EC using 99mTc-glucoheptonate (99mTc-GHA) prepared at pH 6.5, was attempted, prompted by the reported success of the preparation of 99mTc-sestamibi (99mTc-MIBI) by this method. The preparation of 99mTc-EC by this method first involved the formation of 99mTc-GHA by the addition of sodium pertechnetate-99mTc to the Sn-GHA kit vial at pH 6.5. 99mTc-EC was formed by the addition of reconstituted EC solution at pH approximately 12 to the preformed 99mTc-GHA. The reaction was allowed to proceed both at room temperature and on a boiling water bath. The pH of the final product was adjusted to pH approximately 7 with 0.5 M phosphate buffer at pH 4-5, without affecting the quality of the product. The urinary excretion of 99mTc-EC prepared by transchelation, tested in mice, was similar to that of directly prepared 99mTc-EC, indicating that the final product prepared by the two methods was the same. The clinical evaluation of the product formulated by the new procedure showed satisfactory findings, comparable with the reports in the literature.
authors.affiliation: Radiopharmaceuticals Programme, Board of Radiation and Isotope Technology (BRIT), BARC Vashi Complex, Navi, Mumbai, India.
authors.forename: R
authors.initials: R
authors.lastname: Vanaja
authors.forename: S V
authors.initials: SV
authors.lastname: Solav
authors.forename: S H
authors.initials: SH
authors.lastname: Joshi
authors.forename: P
authors.initials: P
authors.lastname: Kumar
authors.forename: N
authors.initials: N
authors.lastname: Ramamoorthy
mesh_terms: D000328:Adult; D000818:Animals; D002614:Chelating Agents; D002648:Child; D003545:Cysteine / Q000031:analogs & derivatives / Q000302:isolation & purification* / Q000493:pharmacokinetics; D004355:Drug Stability; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007668:Kidney / Q000000981:diagnostic imaging*; D007677:Kidney Function Tests / Q000379:methods*; D008297:Male; D051379:Mice; D015609:Organotechnetium Compounds / Q000737:chemistry* / Q000302:isolation & purification* / Q000493:pharmacokinetics; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000302:isolation & purification* / Q000493:pharmacokinetics; D013400:Sugar Acids / Q000737:chemistry*; D014018:Tissue Distribution
chemical_list: D002614:Chelating Agents; D015609:Organotechnetium Compounds; D019275:Radiopharmaceuticals; D013400:Sugar Acids; C109063:ethylene dicysteine; C100014:technetium Tc 99m gluceptate; D003545:Cysteine