title: Airway responses to chronic ozone exposure are partially mediated through mast cells.
abstract: Airways inflammation and epithelial injury induced by chronic ozone (O(3)) in genetically mast cell-deficient mice (Kit(W)/Kit(W-v)) were compared with those in mast cell-sufficient mice (+/+) and Kit(W)/Kit(W-v) mice repleted of mast cells (Kit(W)/Kit(W-v)-BMT). Mice were exposed to 0.26 ppm O(3) 8 h/day, 5 days/wk, for 1-90 days. Background was 0.06 ppm O(3). Age-matched mice were exposed to filtered air for O(3) controls. Reversibility of lesions was evaluated 35 days after exposure. Compared with Kit(W)/Kit(W-v), O(3) caused greater increases in lavageable macrophages, epithelial cells, and polymorphonuclear leukocytes in +/+ and Kit(W)/Kit(W-v)-BMT mice. O(3) also caused lung hyperpermeability, but the genotypic groups were not different. Cells and permeability returned to air control levels after O(3). O(3) induced lung cell proliferation only in +/+ and Kit(W)/Kit(W-v)-BMT mice; proliferation remained elevated or increased in +/+ and Kit(W)/Kit(W-v)-BMT mice after O(3). Greater O(3)-induced cell proliferation was found in nasal epithelium of +/+ and Kit(W)/Kit(W-v)-BMT mice compared with Kit(W)/Kit(W-v) mice. Results are consistent with the hypothesis that mast cells affect airway responses induced by chronic O(3) exposure.
authors.affiliation: Department of Environmental Health Sciences, Division of Physiology, The Johns Hopkins University, Baltimore, Maryland 21205, USA. skleeber@jhsph.edu
authors.forename: S R
authors.initials: SR
authors.lastname: Kleeberger
authors.forename: Y
authors.initials: Y
authors.lastname: Ohtsuka
authors.forename: L Y
authors.initials: LY
authors.lastname: Zhang
authors.forename: M
authors.initials: M
authors.lastname: Longphre
mesh_terms: D000388:Air; D000818:Animals; D001772:Blood Cell Count; D001980:Bronchi / Q000166:cytology / Q000276:immunology; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D002452:Cell Count; D002455:Cell Division; D004847:Epithelial Cells / Q000166:cytology; D007249:Inflammation / Q000209:etiology / Q000276:immunology*; D007958:Leukocyte Count; D008264:Macrophages / Q000166:cytology; D008297:Male; D008407:Mast Cells / Q000166:cytology / Q000276:immunology*; D051379:Mice; D008817:Mice, Mutant Strains; D009297:Nasal Mucosa / Q000166:cytology; D009504:Neutrophils / Q000166:cytology; D010126:Ozone / Q000009:adverse effects*; D020545:Respiratory Mucosa / Q000166:cytology / Q000276:immunology*; D014132:Trachea / Q000166:cytology / Q000276:immunology
chemical_list: D010126:Ozone