Validation of a high-performance liquid chromatographic method for the determination of ibuprofen enantiomers in plasma of broiler chickens.
To characterise the pharmacokinetic properties of each enantiomer of ibuprofen in broiler chickens, a stereospecific HPLC method based on a alpha1-acid glycoprotein bonded chiral stationary phase has been validated. S-(+)-naproxen was used as internal standard. Enantiomers of ibuprofen and S-(+)-naproxen were baseline separated using a mobile phase consisting of 0.1 M phosphate buffer pH = 7 and 0.4% 2-propanol. The method is precise, specific, accurate and reproducible. Recoveries were higher than 80% and the limits of quantification for R-(-)- and S-(+)-ibuprofen were 1.16 and 1.37 microg ml(-1), respectively. The method seemed suitable for the pharmacokinetic studies of ibuprofen in chickens.
Vermeulen
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Laboratory of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Ghent University, Gent, Belgium.
Remon
J P
JP
D019840:2-Propanol / Q000737:chemistry; D000284:Administration, Oral; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood*; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007052:Ibuprofen / Q000097:blood*; D007262:Infusions, Intravenous; D008968:Molecular Conformation; D015203:Reproducibility of Results; D013237:Stereoisomerism
D000894:Anti-Inflammatory Agents, Non-Steroidal; D019840:2-Propanol; D007052:Ibuprofen