Intracellular pH in one-cell mouse embryo differs between subcellular compartments and between interphase and mitosis.
The pH-sensitive dual-emission fluorophore SNARF-1 coupled with a laser confocal microspectrofluorimeter was used to measure the internal pH (pHi) in different subcellular and subnuclear compartments of early mouse embryos. By this method we analysed the first cell cycle of naturally fertilised embryos in order to detect possible pHi changes correlated to cellular events, particularly the onset of replication or transcription and the first mitosis. Throughout interphase, significant differences of pHi were observed between cytoplasm and pronuclei, and, even more striking, between these compartments and nucleolus precursor bodies, whose pHi was systematically lower. We could detect significant pHi change neither during the replication phase nor at the onset of zygotic transcription, but pHi increased at the end of the one-cell stage in both cytoplasm and chromatin regions, a process that seemed specifically correlated with mitosis.
Amirand
C
C
Laboratoire de physicochimie biomoléculaire et cellulaire, ESA 7033 CNRS, université Pierre-et-Marie-Curie, Paris, France.
Mentré
P
P
van de Geijn
S
S
Waksmundzka
M
M
Debey
P
P
D000818:Animals; D001578:Benzopyrans; D002138:Calibration; D002451:Cell Compartmentation / Q000502:physiology*; D002453:Cell Cycle / Q000502:physiology*; D002467:Cell Nucleus / Q000737:chemistry / Q000648:ultrastructure; D002970:Cleavage Stage, Ovum / Q000737:chemistry / Q000166:cytology* / Q000648:ultrastructure; D005456:Fluorescent Dyes; D006863:Hydrogen-Ion Concentration; D007399:Interphase / Q000502:physiology; D051379:Mice; D008808:Mice, Inbred CBA; D008856:Microscopy, Fluorescence / Q000379:methods; D008938:Mitosis / Q000502:physiology; D009284:Naphthols; D012235:Rhodamines
D001578:Benzopyrans; D005456:Fluorescent Dyes; D009284:Naphthols; D012235:Rhodamines; C091666:seminaphthorhodaminefluoride