title: Isolation and purification of the reaction center (RC) and the core (RC-LH1) complex from Rhodobium marinum: the LH1 ring of the detergent-solubilized core complex contains 32 bacteriochlorophylls.
abstract: The reaction center (RC) and the core (RC-LH1) complex were isolated and purified from Rhodobium marinum; together with the LH1 complex [Meckenstock et al. (1992a) FEBS Lett. 311: 128], a complete set of RC, LH1 and RC-LH1 from the same wild-type strain of a purple photosynthetic bacterium can therefore now be made. Comparison of the BChl a/BPhe a ratio (determined by HPLC) between the RC and the RC-LH1 complexes lead us to the determination of the number of BChls in the LH1 ring to be 32.06+/-2.90, indicating that the LH1 ring from Rh. marinum consists of 16 alphabeta subunits.
authors.affiliation: Faculty of Science, Kwansei Gakuin University, Uegahara, Nishinomiya, 662-8501 Japan.
authors.forename: P
authors.initials: P
authors.lastname: Qian
authors.forename: T
authors.initials: T
authors.lastname: Yagura
authors.forename: Y
authors.initials: Y
authors.lastname: Koyama
authors.forename: R J
authors.initials: RJ
authors.lastname: Cogdell
mesh_terms: D001419:Bacteria / Q000737:chemistry*; D001429:Bacteriochlorophylls / Q000737:chemistry*; D003902:Detergents / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry / Q000302:isolation & purification*; D012995:Solubility
chemical_list: D001429:Bacteriochlorophylls; D003902:Detergents; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins