Molecular characterization of the hlyX-like gene of Actinobacillus actinomycetemcomitans Y4.
We isolated and characterized a possible regulatory gene, designated actX gene, from Actinobacillus actinomyctemcomitans Y4, which defined the Actinobacillus pleuropneumoniae hlyX-like regulatory gene. DNA sequence analysis for plasmid clone pKM317 containing a 1.6-kb DNA insert indicated an open reading frame encoding a polypeptide of 257 amino acid residues. Analysis of the deduced amino acid sequence showed the presence of five characteristic cysteine residues in the N-terminal region and a putative DNA binding residue in the C-terminal region, indicating that actX might belong to a regulatory gene family. Escherichia coli DH5alpha and a mutant strain JRG1728 transformed by plasmid carrying actX manifested apparent hemolytic activity on sheep blood agar and grew anaerobically, although the original strains did not.
Kokeguchi
S
S
Department of Periodontology and Endodontology, Okayama University Dental School, Japan.
Hirosue
M
M
Maeda
H
H
Miyamoto
M
M
Takashiba
S
S
Murayama
Y
Y
D016976:Aggregatibacter actinomycetemcomitans / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005809:Genes, Regulator; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D014157:Transcription Factors; D014774:Virulence
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C087924:HlyX protein, Actinobacillus pleuropneumoniae; D014157:Transcription Factors; C428045:actX protein, Actinobacillus actinomycetemcomitans