Binary typing of Staphylococcus aureus strains through reversed hybridization using digoxigenin-universal linkage system-labeled bacterial genomic DNA.
A novel binary typing (BT) procedure, based on reversed hybridization of digoxigenin-universal linkage system-labeled bacterial DNA to strip-immobilized probes, is presented. Chromogenic detection of hybrids was performed. Staphylococcus aureus isolates (n = 20) were analyzed to establish the feasibility of BT. A technically simple and fast procedure has been developed for application in routine microbiology laboratories.
van Leeuwen
W
W
Department of Medical Microbiology & Infectious Diseases, Erasmus University Medical Center Rotterdam, 3015 GD Rotterdam, The Netherlands. vanleeuwen@bacl.azr.nl
Libregts
C
C
Schalk
M
M
Veuskens
J
J
Verbrugh
H
H
van Belkum
A
A
D015373:Bacterial Typing Techniques; D002863:Chromogenic Compounds; D015342:DNA Probes / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics*; D004076:Digoxigenin; D016521:Electrophoresis, Gel, Pulsed-Field; D016680:Genome, Bacterial; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000379:methods; D019105:Random Amplified Polymorphic DNA Technique; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics
D002863:Chromogenic Compounds; D015342:DNA Probes; D004269:DNA, Bacterial; D004076:Digoxigenin