Role of selectins on IgE-mediated skin reaction.
Selectins play an important role on leukocytes infiltration into inflammatory tissues. To understand the role of selectins, we investigated the effects of selectin-IgG chimeras and anti selectin antibodies on the murine IgE-mediated skin inflammation model. Biphasic skin reactions were induced by intradermal challenge with ovalbumin (OA) to ears of actively sensitized mice. This reaction was characterized by immediate and late phase responses observed as which were induced via a rapid increase in capillary permeability and leukocyte infiltration, respectively. The expression of E-selectin mRNA was significantly increased to reach its highest level at 2 h after OA challenge. E-, P-, and L-selectin-IgG chimeras inhibited the late phase responses, i.e. ear swelling, neutrophil infiltration and eosinophil infiltration at 24 h after OA challenge in a dose-dependent manner at dose range of 0.1 - 10 mg kg(-1), i.v. Antiselectin antibodies did not inhibit the increase of ear swelling. But anti E- and P-selectin antibodies significantly inhibited neutrophil infiltration and eosinophil infiltration. These results indicate that selectins play an important role on the late phase response of the murine IgE-mediated skin inflammation model by mediating inflammatory cell adhesion to endothelium.
Wada
Y
Y
R&D Laboratories, Nippon Organon K.K., 5-90. Tomobuti-cho 1-chome Miyakojima-ku, Osaka 534-0016, Japan.
Kuzuhara
A
A
Hanamura
M
M
Kida
R
R
Yoshinaka
T
T
Saito
T
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D019040:E-Selectin / Q000235:genetics / Q000276:immunology / Q000494:pharmacology; D004423:Ear; D004487:Edema / Q000201:enzymology / Q000235:genetics / Q000517:prevention & control; D047209:Eosinophil Peroxidase; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology*; D007074:Immunoglobulin G / Q000235:genetics / Q000494:pharmacology; D007249:Inflammation / Q000473:pathology / Q000517:prevention & control; D007279:Injections, Subcutaneous; D019041:L-Selectin / Q000235:genetics / Q000276:immunology / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology; D019007:P-Selectin / Q000235:genetics / Q000276:immunology / Q000494:pharmacology; D009195:Peroxidase / Q000187:drug effects / Q000378:metabolism; D010544:Peroxidases / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000494:pharmacology; D019087:Selectins / Q000235:genetics / Q000494:pharmacology / Q000502:physiology*; D012867:Skin / Q000187:drug effects / Q000276:immunology* / Q000473:pathology; D013997:Time Factors
D000911:Antibodies, Monoclonal; D019040:E-Selectin; D007074:Immunoglobulin G; D019007:P-Selectin; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D019087:Selectins; D019041:L-Selectin; D007073:Immunoglobulin E; D010047:Ovalbumin; D047209:Eosinophil Peroxidase; D010544:Peroxidases; D009195:Peroxidase