title: Antibody reactivity to Cryptosporidium parvum in saliva of calves after experimental infection.
abstract: Antibodies against Cryptosporidium parvum in the saliva and sera of three calves experimentally infected with this parasite were examined by an indirect immunofluorescence antibody test and immunoblotting. Salivary anti-C. parvum IgA antibody appeared on day 12 post-challenge and had a tendency to increase transiently between days 15 and 30 post-challenge. Salivary anti-C. parvum IgG antibody levels showed a gradual increase along with the change in IgA antibody levels during the infection. In contrast, serum anti-C. parvum IgA antibody levels showed only a slight increase between days 15 and 30 post-challenge. Serum anti-C. parvum IgG antibody levels rose on day 12 post-challenge and one calf maintained relatively high level up to the end of the experiment. In immunoblotting, an antigen with a molecular mass of 15 kDa was found to react strongly to salivary IgA antibody and a 27 kDa antigen to react to serum IgG antibody.
authors.affiliation: Department of Veterinary Physiology, Obihiro University of Agriculture and Veterinary Medicine, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Toyoguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Omata
authors.forename: T
authors.initials: T
authors.lastname: Koyama
authors.forename: T
authors.initials: T
authors.lastname: Kamiyoshi
authors.forename: K
authors.initials: K
authors.lastname: Takeda
authors.forename: H
authors.initials: H
authors.lastname: Furuoka
authors.forename: Y
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: F
authors.initials: F
authors.lastname: Kakuda
authors.forename: R
authors.initials: R
authors.lastname: Maeda
authors.forename: T
authors.initials: T
authors.lastname: Matsui
authors.forename: A
authors.initials: A
authors.lastname: Saito
authors.forename: T
authors.initials: T
authors.lastname: Mikami
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000913:Antibodies, Protozoan / Q000032:analysis* / Q000097:blood; D000917:Antibody Formation; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000276:immunology* / Q000469:parasitology; D003457:Cryptosporidiosis / Q000097:blood / Q000276:immunology / Q000662:veterinary*; D016785:Cryptosporidium parvum / Q000276:immunology*; D019084:Fluorescent Antibody Technique, Indirect; D015151:Immunoblotting; D007070:Immunoglobulin A / Q000032:analysis / Q000097:blood; D008297:Male; D008970:Molecular Weight; D012463:Saliva / Q000276:immunology*; D013997:Time Factors
chemical_list: D000913:Antibodies, Protozoan; D007070:Immunoglobulin A