title: The miaA mutator phenotype of Escherichia coli K-12 requires recombination functions.
abstract: miaA mutants, which contain A-37 instead of the ms(2)i(6)A-37 hypermodification in their tRNA, show a moderate mutator phenotype leading to increased GC-->TA transversion. We show that the miaA mutator phenotype is dependent on recombination functions similar to, but not exactly the same as, those required for translation stress-induced mutagenesis.
authors.affiliation: Department of Microbiology and Molecular Genetics, University of Texas-Houston Medical School, Houston, Texas 77030, USA.
authors.forename: J
authors.initials: J
authors.lastname: Zhao
authors.forename: H E
authors.initials: HE
authors.lastname: Leung
authors.forename: M E
authors.initials: ME
authors.lastname: Winkler
mesh_terms: D000251:Adenosine Triphosphatases; D019883:Alkyl and Aryl Transferases / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D020137:Base Pair Mismatch; D004260:DNA Repair; D004268:DNA-Binding Proteins; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics*; D029968:Escherichia coli Proteins; D051719:MutS DNA Mismatch-Binding Protein; D009154:Mutation; D010641:Phenotype; D012354:RNA, Transfer, His / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic; D013466:Sulfurtransferases / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D012354:RNA, Transfer, His; D019883:Alkyl and Aryl Transferases; C024274:adenylate isopentenyltransferase; C499584:MiaB protein, E coli; D013466:Sulfurtransferases; C402430:isopentenyl-adenosine i6a thiotransferase; D000251:Adenosine Triphosphatases; D051719:MutS DNA Mismatch-Binding Protein; C497175:MutS protein, E coli