Comparative study of Korean (Viscum album var. coloratum) and European mistletoes (Viscum album).
A lectin (agglutinin, VCA) from Korean mistletoe (Viscum album L. coloratum) was isolated by affinity chromatograpy on a asialofetuin-Sepharose 4B. The molecular weights of A- and B-chains of VCA were differenf from those of VAAS. The VCA recognized the antibody of VAAs in the Western blot analysis and ELLA system. We also investigated the synergistic effects of the components in mistletoe by dividing the extract into different molecular weight fractions.
Lyu
S Y
SY
College of Pharmacy, Sook-Myung Womens University, Seoul, Korea.
Park
S M
SM
Choung
B Y
BY
Park
W B
WB
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D015153:Blotting, Western; D004354:Drug Screening Assays, Antitumor; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005060:Europe; D006801:Humans; D007723:Korea; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification; D014784:Mistletoe / Q000737:chemistry*; D008970:Molecular Weight; D010936:Plant Extracts / Q000032:analysis; D037121:Plant Lectins; D010946:Plants, Medicinal; D014407:Tumor Cells, Cultured; D014462:Ultrafiltration
D000972:Antineoplastic Agents, Phytogenic; D037102:Lectins; D010936:Plant Extracts; D037121:Plant Lectins