[Use of transmembrane current harmonics originating from electrostriction for clarifying mechanisms of ion transport in prepartions of sodium-potassium ATPase].
The mechanisms of generation of transmembrane current harmonics arising due to electrostriction of the bilayer upon applying an alternating electric voltage to sodium/potassium ATPase sorbed on the support was studied. It is discussed what information can be obtained by measurements of transmembrane current harmonics to clarify the mechanism of ion transport in sodium/potassium ATPase preparations. It was shown that for studying the current harmonics upon electrostriction, the preparations should be sorbed on bilayer lipid membranes with a high modulus of elasticity. The kinetics of changes in the amplitude of the second current harmonics in response to an impulse addition of ATP to the preparation can have some specific features depending on the mechanism of ion transport.
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ELDIS Research Center, Electronic Diagnostic Systems, Russian Academy of Sciences, Starosadskii per. 8, Moscow, 101000 Russia.
D017136:Ion Transport; D008051:Lipid Bilayers; D008564:Membrane Potentials; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D055672:Static Electricity
D008051:Lipid Bilayers; D000254:Sodium-Potassium-Exchanging ATPase