Estrogen modulates norepinephrine-induced accumulation of adenosine cyclic monophosphate in a subpopulation of immortalized luteinizing hormone-releasing hormone secreting neurons from the mouse hypothalamus.
A subpopulation of luteinizing hormone-releasing hormone (LHRH)-producing cells that express the intermediate filament protein vimentin and the neuronal marker neurofilament 145, but not neurofilament 200 nor glial fibrillary acidic protein, has been isolated from GT1-7 cultures. These cells express the mRNA encoding estrogen receptor alpha (ERalpha) and respond to physiological concentrations of 17beta-estradiol (E2) by reducing the accumulation of cyclic adenosine monophosphate induced by norepinephrine, but not that induced by direct activation of adenylate cyclase. These results indicate that the activity of LHRH-producing neurons may be directly modulated by estrogen. In addition, they are suggestive of an estrogen-dependent desensitization of the beta-adrenoceptor in these cells.
Martínez-Morales
J R
JR
Department of Physiology, Laboratory of Cellular Neurobiology, University of La Laguna School of Medicine, Tenerife, Spain.
Morales
A
A
Marín
R
R
Hernández-Jiménez
J G
JG
Acevedo
A
A
Guerra
B
B
Hernández
G
G
López-Coviella
I
I
Prieto
L
L
Alonso
R
R
D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D000242:Cyclic AMP / Q000096:biosynthesis*; D047628:Estrogen Receptor alpha; D004967:Estrogens / Q000494:pharmacology* / Q000502:physiology; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007987:Gonadotropin-Releasing Hormone / Q000235:genetics / Q000378:metabolism*; D007031:Hypothalamus / Q000166:cytology*; D007150:Immunohistochemistry; D051379:Mice; D016900:Neurofilament Proteins / Q000378:metabolism; D009474:Neurons / Q000378:metabolism* / Q000648:ultrastructure; D009638:Norepinephrine / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013347:Subcellular Fractions / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism
D047628:Estrogen Receptor alpha; D004967:Estrogens; D005904:Glial Fibrillary Acidic Protein; D016900:Neurofilament Proteins; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D014746:Vimentin; C051774:neurofilament protein H; D007987:Gonadotropin-Releasing Hormone; D000242:Cyclic AMP; D009638:Norepinephrine