Generation of a mouse model for arginase II deficiency by targeted disruption of the arginase II gene.
Mammals express two isoforms of arginase, designated types I and II. Arginase I is a component of the urea cycle, and inherited defects in arginase I have deleterious consequences in humans. In contrast, the physiologic role of arginase II has not been defined, and no deficiencies in arginase II have been identified in humans. Mice with a disruption in the arginase II gene were created to investigate the role of this enzyme. Homozygous arginase II-deficient mice were viable and apparently indistinguishable from wild-type mice, except for an elevated plasma arginine level which indicates that arginase II plays an important role in arginine homeostasis.
Shi
O
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Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA.
Morris
S M
SM
Zoghbi
H
H
Porter
C W
CW
O'Brien
W E
WE
D000596:Amino Acids / Q000097:blood; D000818:Animals; D001119:Arginase / Q000235:genetics* / Q000502:physiology; D001120:Arginine / Q000097:blood; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018390:Gene Targeting; D006801:Humans; D020162:Hyperargininemia; D051379:Mice; D018345:Mice, Knockout; D023421:Models, Animal; D010641:Phenotype; D011073:Polyamines / Q000378:metabolism
D000596:Amino Acids; D017931:DNA Primers; D011073:Polyamines; D001120:Arginine; D001119:Arginase