Determination of biogenic amines in fish implicated in food poisoning by micellar electrokinetic capillary chromatography.
A micellar electrokinetic capillary chromatography (MECC) method for the simultaneous determination of seven biogenic amines in fish was developed. The peaks of all components were successfully separated within 11.5 min. MECC was performed with 0.06 M sodium deoxycholate in 0.02 M borate buffer (pH 9.2)-methanol (95:5, v/v) solvent. The average recoveries for all components ranged from 84.4 to 100.3%. The application of this method to detect amines in fried marlin fillet implicated in a food poisoning incident indicated that a high level (56.24 mg/100 g) of histamine was present in the sample. Another 10 fish samples collected from markets were also analyzed and did not contain detectable levels of histamine (<2.5 mg/100 g).
Su
S C
SC
National Laboratories of Foods and Drugs, Department of Health, Execultive Yuan, Taipei, Taiwan.
Chou
S S
SS
Chang
P C
PC
Hwang
D F
DF
D000818:Animals; D001679:Biogenic Amines / Q000032:analysis*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D005396:Fish Products / Q000032:analysis*; D005399:Fishes; D005517:Foodborne Diseases / Q000378:metabolism*; D006801:Humans; D012015:Reference Standards
D001679:Biogenic Amines