Study on the metabolites of 1alpha,25-dihydroxyvitamin D4.
Three major metabolites of 1alpha,25-dihydroxyvitamin D(4) were isolated from the bile of rat and the structures were elucidated on the basis of spectral data and the periodate oxidative cleavage of the diol structures of the metabolites. One of the metabolites was the known calcitroic acid. Another two metabolites were isomers and identified as 9,10-secoergosta-5,7,10(19)-triene-1alpha,3beta,24,25-tetrahydroxy-26-oic acid and 9,10-secoergosta-5,7,10(19)-triene-1alpha,3beta,24,25-tetrahydroxy-28-oic acid. It was found that 1alpha,25-dihydroxyvitamin D(4) is metabolized in a similar manner in vivo to that of 1alpha,25-dihydroxyvitamin D(2) but differently from 1alpha,25-dihydroxyvitamin D(3).
Tachibana
Y
Y
Pharmaceutical Research Center, Nisshin Flour Milling Co., Ltd., 5-3-1 Tsurugaoka, Ooi-machi, Iruma-gun, 356-0045, Saitama, Japan. tachibanay@mail.ni-net.co.jp
Tsuji
M
M
D000818:Animals; D008297:Male; D013058:Mass Spectrometry; D015394:Molecular Structure; D051381:Rats; D017208:Rats, Wistar; D014807:Vitamin D / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism*
C422824:1,25-dihydroxyvitamin D4; D014807:Vitamin D