Differential gene expression of livers from ApoE deficient mice.
A genomic survey for differentially expressed genes was performed to livers of ApoE deficient mice using human cDNA microarray containing approximately 9,000 human cDNA clones. Due to the homology between mouse and human, hybridization was performed at lower stringency condition, 10 degrees below the regular hybridizing temperature. Gene expression profiles of livers corresponding to high levels of blood cholesterol were generated at genomic scale. Thirty-seven genes were randomly selected from a pool of differentially expressed genes and subjected to semi-quantitative RT-PCR, further confirmed the result from microarray hybridization. These included genes associated with atherosclerosis, and novel genes that implied novel pathways correlated to high levels of blood cholesterol. It is promising using human cDNA microarray, the most complete collection among all species, to study other mammalian systems with satisfying speed and accuracy.
Huang
G S
GS
Cardiovascular Research Center, Comparative Medicine, Pig Research Institute Taiwan, Miaoli, Taiwan, ROC. guehuang@ms21.hinet.net
Yang
S M
SM
Hong
M Y
MY
Yang
P C
PC
Liu
Y C
YC
D000818:Animals; D001057:Apolipoproteins E / Q000235:genetics*; D002784:Cholesterol / Q000097:blood; D015870:Gene Expression; D020869:Gene Expression Profiling; D008099:Liver / Q000378:metabolism*; D051379:Mice; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D001057:Apolipoproteins E; D012333:RNA, Messenger; D002784:Cholesterol