Micropatterned solid-supported membranes formed by micromolding in capillaries.
The formation of individually addressable micropatterned solid-supported lipid bilayers has been accomplished by means of micromolding in capillaries. Small unilamellar vesicles were spread on glass slides to form planar supported membranes along microscopic capillaries molded as trenches into a polydimethylsiloxane (PDMS) elastomer. PDMS provides an elastic and transparent carrier for microcapillaries molded from silicon wafers displaying the desired inverse trenches. The so-called master structure has been conventionally etched into silicon by photolithography. The cured PDMS elastomer was briefly exposed to an oxygen plasma, rendering the surface hydrophilic, and subsequently attached to a glass surface in order to form hydrophilic capillaries equipped with flow-promoting pads on either side. One flowpad acts as a reservoir to be filled with the vesicle suspension, while the other one serves as a collector to ensure a sufficient capillary flow to cover the substrate completely. Formation of planar lipid bilayers on the glass slide along the capillaries was followed by imaging the flow and spreading of fluorescently labeled DMPC liposomes with confocal laser scanning microscopy. By means of scanning force microscopy in aqueous solution the formed lipid structures were identified and the height of the lipid bilayers was accurately determined. With both techniques, it was shown that the patterned bilayers remain separated and persist for several hours on the substrate in aqueous solution.
Janshoff
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Institut für Biochemie, Westfälische Wilhelms-Universität, Münster, Germany. janshof@nwz.uni-muenster.de
Künneke
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S
D001672:Biocompatible Materials; D002197:Capillary Action; D004129:Dimethylpolysiloxanes; D004134:Dimyristoylphosphatidylcholine; D004548:Elasticity; D008051:Lipid Bilayers; D008081:Liposomes; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D008954:Models, Biological; D009757:Nylons; D010714:Phosphatidylethanolamines; D014966:Xanthenes
D001672:Biocompatible Materials; D004129:Dimethylpolysiloxanes; D008051:Lipid Bilayers; D008081:Liposomes; D009757:Nylons; D010714:Phosphatidylethanolamines; D014966:Xanthenes; C085707:poly(dimethylsiloxane)-polyamide copolymer; C007510:1,2-dipalmitoyl-3-phosphatidylethanolamine; C034657:Texas red; D004134:Dimyristoylphosphatidylcholine