Extended method for discrimination of Aeromonas spp. by 16S rDNA RFLP analysis.
A previously described molecular method, based on 16S rDNA RFLP analysis, for the identification of Aeromonas spp. was unable to separate the species Aeromonas salmonicida, Aeromonas bestiarum and the recently described Aeromonas popoffii. In this study, the method has been extended with endonucleases AIwNI and PstI for the identification of these species. A molecular frame for the identification of all known Aeromonas spp. is presented.
Figueras
M J
MJ
Soler
L
L
Chacón
M R
MR
Guarro
J
J
Martínez-Murcia
A J
AJ
D000333:Aeromonas / Q000145:classification* / Q000235:genetics*; D000818:Animals; D004275:DNA, Ribosomal / Q000032:analysis*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA
D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; C053688:endodeoxyribonuclease AlwNI; C024388:CTGCAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific