Effect of recombinant human granulocyte colony stimulating factor on lymphocyte blastogenesis in healthy dogs.
Effects of recombinant human granulocyte colony-stimulating factor (rhG-CSF) on the number and blastogenesis of lymphocytes were evaluated in clinically healthy dogs treated subcutaneously with rhG-CSF at a dose of 2.5 microg/kg for 3 days. Significant increases in the number of leukocytes and segmented neutrophils were observed after the administration of rhG-CSF. The number of lymphocytes also increased on days 1 and 2 after the treatment. Activities of phytohemagglutinin, concanavalin A, and pokeweed mitogen-induced lymphocyte blastogenesis (LB) were augmented to twice the pretreatment levels by the administration of rhG-CSF. These results suggested that administration of rhG-CSF activated lymphocyte functions such as LB in healthy dogs.
Hasegawa
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Veterinary Teaching Hospital, Faculty of Agriculture, Miyazaki University, Gakuen-Kibanadai, Japan.
Inomata
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D000818:Animals; D003208:Concanavalin A; D004285:Dogs; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D007279:Injections, Subcutaneous; D007958:Leukocyte Count / Q000662:veterinary; D007962:Leukocytes / Q000166:cytology* / Q000187:drug effects; D008213:Lymphocyte Activation / Q000187:drug effects*; D018655:Lymphocyte Count / Q000662:veterinary; D009504:Neutrophils / Q000166:cytology* / Q000187:drug effects; D010835:Phytohemagglutinins; D011043:Pokeweed Mitogens; D011994:Recombinant Proteins
D010835:Phytohemagglutinins; D011043:Pokeweed Mitogens; D011994:Recombinant Proteins; D003208:Concanavalin A; D016179:Granulocyte Colony-Stimulating Factor