title: Antiproliferative effect and cell cycle modulation by melatonin on GH(3) cells.
abstract: We have investigated the effect of melatonin on cell proliferation and modulation, in the GH(3) experimental rat pituitary cell line; the expression of oncogenes c-myc, c-jun and the tumor suppressor gene p53 were also analyzed basally and after exposure to melatonin (10(-6), 10(-8) and 10(-10) M). Melatonin exhibited an antiproliferative effect at all the doses tested, decreasing the proliferating index by 50%. After exposure to melatonin, a decrease in Ki67 and Proliferation cell nuclear antigen occurred acute- and transiently (at 2 h) after a single dose which recovered at 4 h, as well as chronically after repeated 12-hour doses which persisted at 48 h; a similar behavior was observed both acute- and chronically for c-myc and c-jun, while it was opposite for p53, rising acute- and transiently as well as after repeated exposure. These results demonstrate that melatonin modulates the proliferation mechanisms of the GH(3) cells.
authors.affiliation: Experimental Endocrinology Laboratory, Department of Endocrinology, Research Institute, Hospital de Sant Pau, Autonomous University of Barcelona, Spain.
authors.forename: O
authors.initials: O
authors.lastname: Fornas
authors.forename: M E
authors.initials: ME
authors.lastname: Mato
authors.forename: S M
authors.initials: SM
authors.lastname: Webb
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects; D016758:Genes, jun / Q000235:genetics; D016259:Genes, myc / Q000235:genetics; D016158:Genes, p53 / Q000235:genetics; D019394:Ki-67 Antigen / Q000378:metabolism; D008550:Melatonin / Q000494:pharmacology*; D010902:Pituitary Gland / Q000166:cytology* / Q000502:physiology*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D013997:Time Factors
chemical_list: D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen; D008550:Melatonin