The gene encoding the NdhH subunit of type 1 NAD(P)H dehydrogenase is essential to survival of synechocystis PCC6803.
The physiological function of the type 1 NAD(P)H dehydrogenase (Ndh-1) of Synechocystis sp. PCC6803 has been investigated by inactivating the gene ndhH encoding a subunit of the complex. Molecular analysis of independent transformants revealed that all clones were heteroploid, containing both wild-type and mutant ndhH copies, whatever the metabolic conditions used during genome segregation, including high CO(2) concentration. By replacing the chromosomal copy of the ndhH gene by a plasmidial copy under the control of a temperature-controlled promoter, we induce a conditional phenotype, growth being only possible at high temperature. This clearly shows for the first time that an ndh gene is indispensable to the survival of Synechocystis sp. PCC6803.
Pieulle
L
L
CEA/Cadarache, DSV-DEVM, Laboratoire d'Ecophysiologie de la Photosynthèse, Saint-Paul-lez-Durance, France.
Guedeney
G
G
Cassier-Chauvat
C
C
Jeanjean
R
R
Chauvat
F
F
Peltier
G
G
D002245:Carbon Dioxide / Q000494:pharmacology; D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D004926:Escherichia coli; D005798:Genes, Bacterial; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D021122:Protein Subunits; D011994:Recombinant Proteins / Q000378:metabolism
D021122:Protein Subunits; D011994:Recombinant Proteins; D002245:Carbon Dioxide; D016660:NAD(P)H Dehydrogenase (Quinone)