title: Hay-Wells syndrome is caused by heterozygous missense mutations in the SAM domain of p63.
abstract: Hay-Wells syndrome, also known as ankyloblepharon-ectodermal dysplasia-clefting (AEC) syndrome (OMIM 106260), is a rare autosomal dominant disorder characterized by congenital ectodermal dysplasia, including alopecia, scalp infections, dystrophic nails, hypodontia, ankyloblepharon and cleft lip and/or cleft palate. This constellation of clinical signs is unique, but some overlap can be recognized with other ectodermal dysplasia syndromes, for example ectrodactyly--ectodermal dysplasia--cleft lip/palate (EEC; OMIM 604292), limb--mammary syndrome (LMS; OMIM 603543), acro-dermato-ungual-lacrimal-tooth syndrome (ADULT; OMIM 103285) and recessive cleft lip/palate--ectodermal dysplasia (CLPED1; OMIM 225060). We have recently demonstrated that heterozygous mutations in the p63 gene are the major cause of EEC syndrome. Linkage studies suggest that the related LMS and ADULT syndromes are also caused by mutations in the p63 gene. Thus, it appears that p63 gene mutations have highly pleiotropic effects. We have analysed p63 in AEC syndrome patients and identified missense mutations in eight families. All mutations give rise to amino acid substitutions in the sterile alpha motif (SAM) domain, and are predicted to affect protein--protein interactions. In contrast, the vast majority of the mutations found in EEC syndrome are amino acid substitutions in the DNA-binding domain. Thus, a clear genotype--phenotype correlation can be recognized for EEC and AEC syndromes.
authors.affiliation: Department of Cell and Molecular Pathology, St John's Institute of Dermatology, The Guy's, King's College and St Thomas' Hospitals' Medical School, St Thomas' Hospital, Lambeth Palace Road, London SE1 7EH, UK.
authors.forename: J A
authors.initials: JA
authors.lastname: McGrath
authors.forename: P H
authors.initials: PH
authors.lastname: Duijf
authors.forename: V
authors.initials: V
authors.lastname: Doetsch
authors.forename: A D
authors.initials: AD
authors.lastname: Irvine
authors.forename: R
authors.initials: R
authors.lastname: de Waal
authors.forename: K R
authors.initials: KR
authors.lastname: Vanmolkot
authors.forename: V
authors.initials: V
authors.lastname: Wessagowit
authors.forename: A
authors.initials: A
authors.lastname: Kelly
authors.forename: D J
authors.initials: DJ
authors.lastname: Atherton
authors.forename: W A
authors.initials: WA
authors.lastname: Griffiths
authors.forename: S J
authors.initials: SJ
authors.lastname: Orlow
authors.forename: A
authors.initials: A
authors.lastname: van Haeringen
authors.forename: M G
authors.initials: MG
authors.lastname: Ausems
authors.forename: A
authors.initials: A
authors.lastname: Yang
authors.forename: F
authors.initials: F
authors.lastname: McKeon
authors.forename: M A
authors.initials: MA
authors.lastname: Bamshad
authors.forename: H G
authors.initials: HG
authors.lastname: Brunner
authors.forename: B C
authors.initials: BC
authors.lastname: Hamel
authors.forename: H
authors.initials: H
authors.lastname: van Bokhoven
mesh_terms: D000015:Abnormalities, Multiple / Q000235:genetics* / Q000473:pathology; D000595:Amino Acid Sequence; D000844:Ankylosis; D001483:Base Sequence; D001665:Binding Sites; D001762:Blepharitis; D002648:Child; D002971:Cleft Lip; D002972:Cleft Palate; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins / Q000235:genetics; D005260:Female; D000091344:Filaggrin Proteins; D016147:Genes, Tumor Suppressor; D006579:Heterozygote; D006801:Humans; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000032:analysis; D007633:Keratins / Q000032:analysis; D008297:Male; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D010750:Phosphoproteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012867:Skin / Q000737:chemistry / Q000473:pathology; D013577:Syndrome; D015534:Trans-Activators; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins
chemical_list: C081876:CKAP4 protein, human; D004268:DNA-Binding Proteins; D000091344:Filaggrin Proteins; D007381:Intermediate Filament Proteins; D008565:Membrane Proteins; D010750:Phosphoproteins; C115051:TP63 protein, human; D015534:Trans-Activators; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; D007633:Keratins; D004247:DNA