title: The effects of cryopreservation regimens on the morphology of human ovarian tissue.
abstract: Human ovarian tissue consisting of stroma, pre-granulosa cells and oocytes, has been frozen using a variety of cooling rates and dehydration regimens. The differential survival of the various cell types under these conditions highlights the difficulty in defining optimum protocols for the cryopreservation of multicellular tissue.
authors.affiliation: Reproductive Biology Unit, Royal Womens Hospital, Carlton, Victoria, Australia. gookd@cryptic.rch.unimelb.edu.au
authors.forename: D A
authors.initials: DA
authors.lastname: Gook
authors.forename: D H
authors.initials: DH
authors.lastname: Edgar
authors.forename: C
authors.initials: C
authors.lastname: Stern
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D015925:Cryopreservation / Q000379:methods* / Q000592:standards; D003890:Desiccation / Q000379:methods; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005260:Female; D006107:Granulosa Cells / Q000166:cytology; D006801:Humans; D009865:Oocytes / Q000166:cytology; D010053:Ovary / Q000166:cytology*; D011247:Pregnancy; D019946:Propylene Glycol / Q000494:pharmacology; D017154:Stromal Cells / Q000166:cytology; D013395:Sucrose / Q000494:pharmacology; D013696:Temperature
chemical_list: D013395:Sucrose; D019946:Propylene Glycol; D004121:Dimethyl Sulfoxide