Specific staining of Sertoli cell nuclei and evaluation of Sertoli cell number and proliferative activity in Meishan and White Composite boars during the neonatal period.
The positive relationship between Sertoli cell number and testicular size emphasizes the importance of determining factors involved in the regulation of the Sertoli cell population. Based on data from other species and indirect evidence in the boar, it is generally accepted that porcine Sertoli cells proliferate rapidly throughout the early postnatal period. However, direct evaluation of Sertoli cell number and the proliferative activity of Sertoli cells during the early postnatal period in boars have not been reported. Stereological enumeration of Sertoli cells is a labor-intensive process and would be greatly facilitated by a marker for these cells especially in the sexually mature male. Thus, the first objective of this study was to determine if expression of the transcription factor GATA-4 is an effective marker for fetal, postnatal, and adult Sertoli cells to facilitate enumeration procedures. The second objective was to evaluate the proliferative activity and growth of the Sertoli cell population in neonatal White Composite and Meishan boars, known to differ in mature testis size and Sertoli cell number, to determine the importance of this developmental period for the adult Sertoli cell population. GATA-4 was abundantly expressed by Sertoli cells throughout fetal and prepubertal stages of development and specifically stained both type A and B Sertoli cell nuclei in the sexually mature boar. Immunoreactivity was never observed in the germ cells regardless of their stage of development, illustrating that GATA-4 is a useful marker for both developing and adult Sertoli cells in the boar. Testicular size did not differ between breeds on Day 1 postpartum, but by 14 days postpartum White Composite boars had significantly larger testes compared to Meishan boars (P: < 0.001). Similarly, Sertoli cell number did not differ between breeds at 1 day postpartum; however, at 14 days postpartum White Composite boars had a significantly larger Sertoli cell population compared to Meishan boars (P: < 0.05). Surprisingly, despite having more Sertoli cells than Meishan boars at 14 days postpartum, the proportion of actively proliferating Sertoli cells in the White Composite boars was almost 50% less than the Meishan boars. This result illustrates that rapid rates of Sertoli cell proliferation probably occurred prior to 14 days postpartum in the White Composite boars. Collectively, these results illustrate that the relationship between testicular size and Sertoli cell number is manifested very early in the postnatal period for these two breeds. The substantial difference in the size of the Sertoli cell population and their proliferative activity between Meishan and White Composite boars during the early postnatal period emphasizes the importance of this early period for the establishment of the Sertoli cell population and subsequent adult testicular size.
McCoard
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SA
USDA, ARS, RLH US Meat Animal Research Center, Reproduction Research Unit, P.O. Box 166, State Spur 18D, Clay Center, NE 68933, USA. mccoard@email.marc.usda.gov
Lunstra
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DD
Wise
T H
TH
Ford
J J
JJ
D000818:Animals; D000831:Animals, Newborn; D002452:Cell Count; D002455:Cell Division / Q000502:physiology; D002467:Cell Nucleus / Q000648:ultrastructure*; D004396:Coloring Agents; D004268:DNA-Binding Proteins / Q000378:metabolism; D005260:Female; D050991:GATA4 Transcription Factor; D005854:Germ Cells / Q000502:physiology; D007150:Immunohistochemistry; D008297:Male; D009929:Organ Size / Q000502:physiology; D011247:Pregnancy; D012708:Sertoli Cells / Q000648:ultrastructure*; D013045:Species Specificity; D013552:Swine; D013737:Testis / Q000166:cytology* / Q000254:growth & development / Q000648:ultrastructure*; D014157:Transcription Factors / Q000378:metabolism
D004396:Coloring Agents; D004268:DNA-Binding Proteins; D050991:GATA4 Transcription Factor; D014157:Transcription Factors