Effects of copper ions on the free radical-scavenging properties of reduced gluthathione: implications of a complex formation.
The interaction between glutathione (GSH) and copper ions was investigated in vitro to determine whether such interaction could affect the free-radical scavenging properties of the tripeptide. To this end, the bleaching (decrease in OD734 nm) of a coloured solution containing the stable free-radical cation ABTS+, (which results from the addition of thiols to such a solution) was employed as an in vitro indication of the ability of the tripeptide to scavenge free radicals. While GSH bleached concentration-dependently (1.0-7.5 gM) the ABTS+-containing solution, its prior incubation (5 microM) in the presence of Cu+1 or Cu+2 ions (1-7.5 M) led to a metal concentration-dependent decrease of the bleaching capacity. At a ratio equal to one (5 microM each), the bleaching capacity of the copper plus GSH mixture was 50% of that seen for GSH alone. Further additions of copper (reaching ratios up to 2) did not result in greater decreases in the GSH-bleaching capacity. Noteworthy at the ratio of onewas that the copper plus GSH solutions maintained their bleaching capacity despite the lack of any DTNB-reactivity, i. e., the complete absence of thiols in the mixture. Mixtures of increasing concentrations of a fixed ratio (equal to 2) of copper plus GSH, which were found not to exhibit any DTNB-reactivity, showed a linear and concentration-dependent increase in bleaching capacity. The bleaching capacity remained unaltered when TRIEN, EDTA or histidine were added to pre-incubated (1:1) mixtures of copper plus GSH. However, the incubation of copper with TRIEN or EDTA (but not histidine) prior to GSH addition, totally prevented the loss of the original GSH-bleaching capacity. The present data supports the formation of a copper-glutathione complex which is stable to the presence of some copper-chelators, lacks all thiol reactivity, but fully conserves the free-radical scavenging properties of GSH.
Jiménez
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I
Nutritional Toxicology Unit, Nutrition and Food Technology Institute, University of Chile, Santiago.
Speisky
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H
D052160:Benzothiazoles; D002412:Cations; D002614:Chelating Agents / Q000737:chemistry; D003300:Copper / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000737:chemistry*; D005978:Glutathione / Q000737:chemistry*; D007202:Indicators and Reagents / Q000737:chemistry; D007700:Kinetics; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013451:Sulfonic Acids / Q000737:chemistry
D052160:Benzothiazoles; D002412:Cations; D002614:Chelating Agents; D016166:Free Radical Scavengers; D007202:Indicators and Reagents; D013438:Sulfhydryl Compounds; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D003300:Copper; D005978:Glutathione