Involvement of transglutaminase in the receptor-mediated endocytosis of mouse peritoneal macrophages.
We investigated the effect of extracellular Ca2+ on the uptake of immunoglobulin G (IgG)-coated normal sheep red blood cells (SRBC) by the mouse peritoneal macrophages. The uptake of SRBC was dose dependently enhanced by Ca2+ concentration. We also investigated the effect of ryanodine on the uptake of SRBC. Ryanodine showed approximately 15% inhibition of the uptake. The activity of transglutaminase (TGase) in the cytosolic fraction of macrophages was enhanced as the Ca2+ concentration increased. The uptake of SRBC decreased by the addition of histamine, a TGase inhibitor. These results suggest that TGase is involved in the receptor-mediated endocytosis system of mouse peritoneal macrophages.
Abe
S
S
Department of Radiopharmacy, Tohoku Pharmaceutical University, Sendai, Japan. shin-abe@tohoku-pharm.ac.jp
Yamashita
K
K
Kohno
H
H
Ohkubo
Y
Y
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D004705:Endocytosis / Q000187:drug effects / Q000502:physiology*; D004912:Erythrocytes / Q000276:immunology / Q000378:metabolism; D006632:Histamine / Q000494:pharmacology; D007074:Immunoglobulin G / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000201:enzymology / Q000276:immunology / Q000502:physiology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D011961:Receptors, Fc / Q000502:physiology*; D012756:Sheep; D011503:Transglutaminases / Q000037:antagonists & inhibitors / Q000378:metabolism*
D007074:Immunoglobulin G; D011961:Receptors, Fc; D006632:Histamine; D011503:Transglutaminases; D002118:Calcium