title: Selectin receptor-ligand bonds: Formation limited by shear rate and dissociation governed by the Bell model.
abstract: We have studied the principles that govern the formation and dissociation of an adhesive bond between a cell moving in shear flow and a substrate and tested different theories of how force affects bond dissociation. Viscosity relates the kinematics of fluid movement (shear rate, units of time(-1)) to shear stress (units of force/area, the product of shear rate and viscosity). At different medium viscosities, the formation of receptor-ligand bonds between a cell in the flowstream and P-selectin on the vessel wall showed a similar efficiency as a function of shear rate but not of shear stress. Therefore, bond formation was a function of shear rate and hence of the kinematics of receptor and ligand movement. By contrast, the kinetics of bond dissociation was a function of shear stress and hence of force on the bond. The different requirements for bond formation and dissociation allowed dissociation kinetics to be measured at higher forces on the bond by increasing medium viscosity. Data over an extended range of forces on the bond therefore could be collected that enabled five different proposed equations, relating force to bond dissociation, to be compared for fit to experimental data. The relationship proposed by Bell [Bell, G. I. (1978) Science 200, 618-627] fit the data significantly the best and also predicted an off-rate in the absence of force that best matched an independent measurement [Mehta, P., Cummings, R. D. & McEver, R. P. (1998) J. Biol. Chem. 273, 32506-32513].
authors.affiliation: The Center for Blood Research, Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.
authors.forename: S
authors.initials: S
authors.lastname: Chen
authors.forename: T A
authors.initials: TA
authors.lastname: Springer
mesh_terms: D001665:Binding Sites; D002634:Chemotaxis, Leukocyte / Q000187:drug effects / Q000502:physiology*; D005362:Ficoll / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D008024:Ligands; D018715:Microscopy, Video; D008954:Models, Biological; D009504:Neutrophils / Q000502:physiology*; D019007:P-Selectin / Q000097:blood* / Q000737:chemistry*; D013314:Stress, Mechanical; D014783:Viscosity
chemical_list: D008024:Ligands; D019007:P-Selectin; D005362:Ficoll