title: Lateral-flow assay for rapid serodiagnosis of human leptospirosis.
abstract: An assay device for the rapid detection of Leptospira-specific immunoglobulin M (IgM) antibodies in human sera is presented. The sensitivity (85.8%) and specificity (93.6%) of the assay compared well (91.9% agreement) with those of an IgM enzyme-linked immunosorbent assay routinely used in the serodiagnosis of leptospirosis. The sensitivity of the assay varied with the stage of the disease. The assay uses stabilized components and is simply performed by the addition of serum and sample fluid to the sample well of the assay device. The assay is read after 10 min, and a positive result is obtained when staining of the test line is observed.
authors.affiliation: Department of Biomedical Research, Royal Tropical Institute, Amsterdam, The Netherlands. H.Smits@kit.nl
authors.forename: H L
authors.initials: HL
authors.lastname: Smits
authors.forename: C K
authors.initials: CK
authors.lastname: Eapen
authors.forename: S
authors.initials: S
authors.lastname: Sugathan
authors.forename: M
authors.initials: M
authors.lastname: Kuriakose
authors.forename: M H
authors.initials: MH
authors.lastname: Gasem
authors.forename: C
authors.initials: C
authors.lastname: Yersin
authors.forename: D
authors.initials: D
authors.lastname: Sasaki
authors.forename: B
authors.initials: B
authors.lastname: Pujianto
authors.forename: M
authors.initials: M
authors.lastname: Vestering
authors.forename: T H
authors.initials: TH
authors.lastname: Abdoel
authors.forename: G C
authors.initials: GC
authors.lastname: Gussenhoven
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood*; D006801:Humans; D007919:Leptospira / Q000276:immunology*; D007922:Leptospirosis / Q000175:diagnosis*; D012698:Serologic Tests / Q000379:methods
chemical_list: D000907:Antibodies, Bacterial