title: A comparative study on potential cytogenetic fingerprints for radiation LET in human lymphocytes.
abstract: OBJECTIVE
  To carry out a comparative study on potential cytogenetic fingerprints for radiation LET in human metaphase lymphocytes.
  
  
  METHODS
  Human lymphocytes were irradiated in vitro with 3.0 Gy 60Co gamma-rays, 0.9 Gy 3H beta-rays or 0.2 Gy 2.7 Mev neutrons. Detailed chromosome aberrations were analysed by combined FISH with pan-telomere staining and specific whole-chromosome painting (1, 2 and 4). Total chromosome translocations and insertions were also analysed by multicolour whole-chromosome painting (chromosomes 1, 2 and 4 orange, chromosomes 3, 5 and 6 green).
  
  
  RESULTS
  Among the six proposed radiation cytogenetic fingerprints, the ratio of total simple translocations to insertions (I-ratio), showed the largest difference between low-LET 60Co gamma-ray and high-LET neutron radiation. The ratios of complete exchanges to incomplete rejoinings [S(I)-ratio] and dicentrics to interstitial deletions (H-ratio), showed a similar significant difference between low- and high-LET radiation. The ratios of centric rings to interstitial deletion (G-ratio) showed a trend of LET-related difference, but the difference was not significant in this data set. The ratios of dicentrics to centric rings (F-ratio) and apparent complete exchanges to hidden complete exchanges [S(II)-ratio], showed no difference between low- and high-LET radiation. In the 1426 radiation-induced chromosome aberrations observed after 52 h culture, evidence for sister-chromatid fusion but not telomere addition was found.
  
  
  CONCLUSIONS
  Pan-telomere staining plus specific whole chromosome painting allows simultaneous and objective detection of complete or incomplete chromosome exchanges and interstitial or terminal deletions in human peripheral lymphocytes. Of the six proposed cytogenetic ratios, the I-ratio is the most effective cytogenetic fingerprint for distinguishing low-LET from high-LET radiation in human metaphase human lymphocytes.
authors.affiliation: University of California,  Livermore 94551, USA.
authors.forename: W
authors.initials: W
authors.lastname: Deng
authors.forename: D P
authors.initials: DP
authors.lastname: Morrison
authors.forename: K L
authors.initials: KL
authors.lastname: Gale
authors.forename: J N
authors.initials: JN
authors.lastname: Lucas
mesh_terms: D001610:Beta Particles; D002869:Chromosome Aberrations; D020223:Chromosome Painting; D002875:Chromosomes / Q000528:radiation effects / Q000648:ultrastructure; D003582:Cytogenetics; D016172:DNA Fingerprinting; D005720:Gamma Rays; D005821:Genetic Techniques; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D018499:Linear Energy Transfer; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D008677:Metaphase; D009502:Neutrons; D016615:Telomere / Q000648:ultrastructure; D013997:Time Factors; D014178:Translocation, Genetic