[Study of thymocytes resistance to ionising irradiation in vitro].
The influence of ionising irradiation with doses of 0.5, 1, 2 Gy upon the suspension of mice thymocytes was studied. The state of the system of lipid peroxidation-antioxidation activity and structural and functional state of cell membrane was studied. It was found that, 15 minutes' after irradiation POL processes are within the norm. But the content of vitamins A and E decreases (the former after 0.5 Gy, the latter after 1 Gy). Changes in structural and functional state of thymocyte membranes can be observed by using fluorescent probes. Particularly, it turned out that after irradiation accessibility of incorporated proteins increases, the polarity of lipids increases and the lipophility of membrane lipids decreases. These changes can lead to short-time increase in specific functional activity of cells, but they can cause rapid death of cells as well.
Paranich
A V
AV
Kharkov National University, Department of Physical Organic Chemistry, Institute for Chemistry, Kharkov, 61077 Ukraine.
Popov
N N
NN
Roshal'
A D
AD
Zhivotova
E N
EN
Grigorovich
A V
AV
D000222:Adaptation, Physiological; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D016923:Cell Death; D002462:Cell Membrane / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D002478:Cells, Cultured / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D015227:Lipid Peroxidation / Q000528:radiation effects; D008563:Membrane Lipids / Q000378:metabolism; D051379:Mice; D011833:Radiation Injuries, Experimental / Q000378:metabolism* / Q000473:pathology; D011839:Radiation, Ionizing; D013950:Thymus Gland / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*
D000975:Antioxidants; D008563:Membrane Lipids