A population analysis of the structure and variability of NOR in Salmo trutta by Ag, CMA3 and ISH.
An analysis of the variation in the number and location of rDNA genes has been carried out in two populations of brown trout (Salmo trutta) from Poland by using Ag and CMA3-staining, and rDNA in situ hybridisation. We observed an interindividual variation in arm number with NF = 100, 101, and 102. This variation was connected with the size polymorphism of the short (NOR-bearing) arm of the chromosome pair 11. The population studied showed a multichromosomal distribution of active NORs. Atypical Ag-NORs consisted of rDNA genes, as evidenced by rDNA-ISH. In addition to individuals with standard NORs, specimens with extra NORs as well as others with only one active NOR and single interphase nucleolus were observed.
Woznick
P
P
Olsztyn University, Departiment of Evolutionary Ecology, Olsztyn-Kortowo, Poland.
Sanchez
L
L
Martinez
P
P
Pardo
B G
BG
Jankun
M
M
D000818:Animals; D014128:Chromomycin A3; D004275:DNA, Ribosomal / Q000235:genetics; D014644:Genetic Variation; D005828:Genetics, Population; D017404:In Situ Hybridization, Fluorescence; D009697:Nucleolus Organizer Region / Q000235:genetics*; D011044:Poland; D012834:Silver; D013194:Staining and Labeling; D014337:Trout / Q000235:genetics*
D004275:DNA, Ribosomal; D012834:Silver; D014128:Chromomycin A3