Impact of antiandrogen treatment on the fatty acid profile of neutral lipids in human meibomian gland secretions.
The purpose of this study was to determine whether the use of antiandrogen medications is associated with significant alterations in the fatty acid (FA) profiles of neutral lipids in human meibomian gland secretions. Meibomian gland secretions were obtained from both eyes of patients receiving antiandrogen therapy and from age-related controls. Samples were processed for high-performance liquid chromatography/mass spectrometry and an evaluation of the mass/charge ratios of neutral lipid FA. Our results demonstrate that antiandrogen therapy is associated with significant and consistent alterations in the mass/charge ratios of neutral lipid fractions of meibomian gland secretions. Patients taking antiandrogen medications had significant changes in the occurrence of numerous diglyceride, triglyceride, and wax/cholesterol ester FA products, compared with age-matched controls. Statistical analyses of data within groups demonstrated very high correlation coefficients, and cross-correlation analyses revealed characteristic shifts in FA patterns between groups. Our findings show that antiandrogen use is paralleled by significant changes in the FA profiles of neutral lipid fractions in meibomian gland secretions.
Sullivan
B D
BD
Schepens Eye Research Institute, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
Evans
J E
JE
Krenzer
K L
KL
Reza Dana
M
M
Sullivan
D A
DA
D000368:Aged; D000465:Algorithms; D000726:Androgen Antagonists / Q000009:adverse effects*; D002788:Cholesterol Esters / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004075:Diglycerides / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry; D008297:Male; D013058:Mass Spectrometry; D008537:Meibomian Glands / Q000187:drug effects / Q000378:metabolism*; D008875:Middle Aged; D014280:Triglycerides / Q000378:metabolism
D000726:Androgen Antagonists; D002788:Cholesterol Esters; D004075:Diglycerides; D005230:Fatty Acids, Nonesterified; D008055:Lipids; D014280:Triglycerides