[Antigen presenting role of dendritic cell proliferated from peripheral blood monocytes in patients with chronic hepatitis B].
OBJECTIVE
To study the presenting effect of dendritic cell (DC) proliferated from peripheral blood monocytes in patients with chronic hepatitis B on HBsAg.


METHODS
DC proliferated from patient's peripheral blood monocytes by adding GM-CSF and IL-4 were incubated with HBsAg for 1.5h at different concentrations, then mixed with autologous T cell and cocultured in 96-well flat-bottomed microtiter plates for 5 d at 37 degrees C and 5% CO(2). (3)H-TdR was added 13h before the culture completion, and finally the cells were gathered and the cpm was tested.


RESULTS
The proliferation of T cell by DC treated by HBsAg is significantly higher than untreated.


CONCLUSIONS
DC cultured from peripheral blood monocytes in patients with chronic hepatitis B plays a role in presenting HBsAg.
Li
Y
Y
Viral Hepatitis Institute of Chongqing University of Medical Sciences, Chongqing 400010, China.
Lou
Y
Y
Liang
Z
Z
Ren
H
H
D017951:Antigen Presentation; D003713:Dendritic Cells / Q000502:physiology*; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D019694:Hepatitis B, Chronic / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation; D013601:T-Lymphocytes / Q000276:immunology*
D006514:Hepatitis B Surface Antigens