title: Perinatal development of the rat kidney: proliferative activity and epidermal growth factor.
abstract: The immunolocalization of proliferating cell nuclear antigen (PCNA), epidermal growth factor (EGF) and its receptor (EGFR) was examined in the perinatal rat kidney. As the index of proliferative activity, PCNA-positive cell ratios in glomeruli and proximal tubules were determined. The PCNA-positive ratios in both glomeruli and proximal tubules decreased significantly during the perinatal period and tended to decrease as the glomerular developmental stage proceeded as well. PCNA-positive cells were seen predominantly in the nephrogenic zone of the kidney throughout the period examined. They were noted in the collecting ducts of the nephrogenic zone and were rarely seen in those of the central zone of the kidney. On the other hand, PCNA-positive cells were noted in the straight portion of the proximal tubules and were rarely seen in the convoluted one. EGF-positive cells were seen in the proximal tubules, distal tubules and collecting ducts, though EGF-positive cells in the proximal tubules decreased after birth. EGFR-positive cells were seen along the entire length of the proximal tubules and collecting ducts as well as in immature glomeruli, not in mature ones. These results indicate that marked cell proliferation occurs in the collecting ducts in the peripheral area and in the proximal tubules in the central area of the kidney, that the proliferative activity decreases with age during the perinatal days and that EGF plays an important role in the proliferation of glomerular cells, and in both proliferation and maturation of the cells in the proximal tubules and collecting ducts.
authors.affiliation: Department of Laboratory Animal Science, Division of Veterinary Science, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Sakai, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Okada
authors.forename: A
authors.initials: A
authors.lastname: Iwamoto
authors.forename: K
authors.initials: K
authors.lastname: Kusakabe
authors.forename: M
authors.initials: M
authors.lastname: Mukamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Kiso
authors.forename: H
authors.initials: H
authors.lastname: Morioka
authors.forename: F
authors.initials: F
authors.lastname: Sasaki
authors.forename: Y
authors.initials: Y
authors.lastname: Morikawa
mesh_terms: D000818:Animals; D002455:Cell Division; D004815:Epidermal Growth Factor / Q000032:analysis* / Q000235:genetics; D066246:ErbB Receptors / Q000032:analysis; D005865:Gestational Age; D007150:Immunohistochemistry; D007668:Kidney / Q000737:chemistry / Q000196:embryology* / Q000254:growth & development*; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D011498:Protein Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D011498:Protein Precursors; D012333:RNA, Messenger; D004815:Epidermal Growth Factor; D066246:ErbB Receptors