Hair cell and supporting cell density and distribution in the normal and regenerating posterior crista ampullaris of the pigeon.
The numbers of supporting cells and the numbers and types of hair cells in three distinct longitudinal regions through the posterior canal cristae of control and streptomycin-treated pigeons were determined using stereological techniques. For control cristae, type I (3758) and type II (3517) hair cells occurred in approximately equal numbers. However, the proportions varied in different longitudinal zones: Zone I (peripheral region) had four times more type II hair cells (2083) than type I (483), while Zone II (intermediate region) had almost seven times more type I (2517) than type II (367) hair cells and Zone III (central region) had relatively equal numbers of type I (758) and type II (1067) hair cells. Novel findings included the following: (1) immediately after the post-injection sequence (PIS) of streptomycin, there was a significant reduction in both hair cells (-93%) and supporting cells (-45%); (2) by 70 days after the PIS, the population of type I hair cells returned to control values (however, the normal complement of complex calyces took 1 year to recover); (3) during the first 143 days after the PIS, the number of type I and type II hair cells across all zones returned linearly with about the same slope (46 and 43 cells per day, respectively), although the rate of return differed significantly in different zones; (4) there was a massive overproduction of hair cells (+150%) and supporting cells (+120%) during the first 5 months of recovery; and (5) during the first year after the PIS, both hair cells and supporting cells increased and their increases in numbers were correlated (r = 0.88, P < 0.01). Knowledge of the sequence and numbers of regenerating hair cells may help elucidate common modes of cell survival, recovery, and compensation from neural insult.
Kevetter
G A
GA
Department of Otolaryngology, University of Texas Medical Branch, Galveston 77555-1063, USA. galeonar@utmb.edu
Blumberg
K R
KR
Correia
M J
MJ
D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity; D002452:Cell Count; D010856:Columbidae; D018069:Hair Cells, Vestibular / Q000166:cytology*; D009410:Nerve Degeneration / Q000139:chemically induced / Q000503:physiopathology; D009416:Nerve Regeneration / Q000502:physiology*; D012665:Semicircular Canals / Q000166:cytology* / Q000502:physiology*; D013307:Streptomycin / Q000633:toxicity; D014725:Vestibular Nerve / Q000166:cytology / Q000502:physiology
D000900:Anti-Bacterial Agents; D013307:Streptomycin