Decreased levels of complex III core protein 1 and complex V beta chain in brains from patients with Alzheimer's disease and Down syndrome.
Ubiquinol:cytochrome c oxidoreductase (complex III) and ATP synthase (complex V) are important enzymes in the mitochondrial electron transport chain. Defects in mitochondrial respiratory enzymes have been reported for several neurodegenerative diseases. In this study, we applied the proteomic approach to investigate protein levels of complex III core protein and complex V beta chain in brain regions of Alzheimer's disease (AD) and Down syndrome (DS) patients. Complex III core protein 1 was significantly reduced in the temporal cortex of AD patients. Complex V beta chain was significantly reduced in the frontal cortex of DS patients. We conclude that decreased mitochondrial respiratory enzymes could contribute to the impairment of energy metabolism observed in DS. These decreases could also cause the generation of reactive oxygen species and neuronal cell death (apoptosis) in DS as well as AD.
Kim
S H
SH
Department of Pediatrics, University of Vienna, Austria.
Vlkolinsky
R
R
Cairns
N
N
Lubec
G
G
D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000378:metabolism*; D000328:Adult; D000368:Aged; D000544:Alzheimer Disease / Q000201:enzymology* / Q000209:etiology / Q000473:pathology; D017209:Apoptosis; D001921:Brain / Q000201:enzymology*; D002352:Carrier Proteins; D016022:Case-Control Studies; D004314:Down Syndrome / Q000201:enzymology* / Q000209:etiology / Q000473:pathology; D014450:Electron Transport Complex III / Q000737:chemistry / Q000378:metabolism*; D004734:Energy Metabolism; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D008875:Middle Aged; D008928:Mitochondria / Q000201:enzymology; D025261:Mitochondrial Proton-Translocating ATPases; D021122:Protein Subunits; D006180:Proton-Translocating ATPases / Q000737:chemistry / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D014018:Tissue Distribution; D025262:Vacuolar Proton-Translocating ATPases
D002352:Carrier Proteins; D008565:Membrane Proteins; D021122:Protein Subunits; D017382:Reactive Oxygen Species; D000251:Adenosine Triphosphatases; D025262:Vacuolar Proton-Translocating ATPases; D025261:Mitochondrial Proton-Translocating ATPases; D006180:Proton-Translocating ATPases; D014450:Electron Transport Complex III; C020439:oligomycin sensitivity-conferring protein