title: HLA class II gene polymorphisms in antiphospholipid syndrome: haplotype analysis in 83 Caucasoid patients.
abstract: OBJECTIVE
  We investigated the association between HLA class II haplotypes and antiphospholipid syndrome (APS).
  
  
  METHODS
  HLA DRB1, DQB1 and DQA1 genotypes were determined by the polymerase chain reaction using sequence-specific primers in 83 Caucasoid British patients with APS. The genotype frequencies were compared between subgroups of patients and 177 healthy controls.
  
  
  RESULTS
  DQB1*0604/5/6/7/9-DQA1*0102-DRB1*1302 and DQB1*0303-DQA1*0201-DRB1*0701 haplotypes showed significantly positive correlations with APS [P=0.0087 and P=0.0012, respectively]. The association of the former was enhanced in primary APS patients with anti-beta 2-glycoprotein I antibodies (anti-beta 2GPI) [odds ratio 6.2, 95% confidence interval (2.2-17.6), P=0.0014, corrected P=0.042].
  
  
  CONCLUSIONS
  These alleles and haplotypes might affect anti-ss2GPI production and APS development in different and heterogeneous fashion.
authors.affiliation: Lupus Research Unit, The Rayne Institute, St Thomas' Hospital, London, UK.
authors.forename: R
authors.initials: R
authors.lastname: Caliz
authors.forename: T
authors.initials: T
authors.lastname: Atsumi
authors.forename: E
authors.initials: E
authors.lastname: Kondeatis
authors.forename: O
authors.initials: O
authors.lastname: Amengual
authors.forename: M A
authors.initials: MA
authors.lastname: Khamashta
authors.forename: R W
authors.initials: RW
authors.lastname: Vaughan
authors.forename: J S
authors.initials: JS
authors.lastname: Lanchbury
authors.forename: G R
authors.initials: GR
authors.lastname: Hughes
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D016736:Antiphospholipid Syndrome / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005820:Genetic Testing; D006683:HLA-DQ Antigens / Q000235:genetics; D059848:HLA-DQ alpha-Chains; D059866:HLA-DQ beta-Chains; D006684:HLA-DR Antigens / Q000235:genetics; D059811:HLA-DRB1 Chains; D006239:Haplotypes; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D044465:White People / Q000235:genetics*
chemical_list: D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; D059866:HLA-DQ beta-Chains; C069050:HLA-DQA1 antigen; C069051:HLA-DQB1 antigen; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D000949:Histocompatibility Antigens Class II