The Chemical Shift Index method applied to resin-bound peptides.
The Chemical Shift Index (CSI) method proposed by Wishart et al. [Biochemistry (1992) 31, 1647-1651] to evaluate the secondary structure of peptides in aqueous solution uses as its reference the chemical shift values of each of the 20 natural amino acids (X) in a typical nonstructured sequence GGXAGG (17-20). In order to apply the CSI method to protected resin-bound peptides, we established a new database of chemical shift values for the same GGXAGG sequences in their protected form and anchored to a polystyrene resin swollen in DMF-d7. The predictive value of this new reference set in the CSI protocol was tested on different resin-bound peptides that were previously characterized by a full NOE analysis.
Fruchart
J S
JS
UMR 8525, CNRS-Université de Lille 2-Institut de Biologie et Institut Pasteur de Lille, France.
Lippens
G
G
Warrass
R
R
Seetharaman
C
C
Dhalluin
C
C
Boutillon
C
C
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008956:Models, Chemical; D008969:Molecular Sequence Data; D010452:Peptide Biosynthesis; D017433:Protein Structure, Secondary; D012116:Resins, Plant / Q000737:chemistry*
D000596:Amino Acids; D012116:Resins, Plant