The transcriptional program of a human B cell line in response to Myc.
The proto-oncogene c-myc (myc) encodes a transcription factor (Myc) that promotes growth, proliferation and apoptosis. Myc has been suggested to induce these effects by induction/repression of downstream genes. Here we report the identification of potential Myc target genes in a human B cell line that grows and proliferates depending on conditional myc expression. Oligonucleotide microarrays were applied to identify downstream genes of Myc at the level of cytoplasmic mRNA. In addition, we identified potential Myc target genes in nuclear run-on experiments by changes in their transcription rate. The identified genes belong to gene classes whose products are involved in amino acid/protein synthesis, lipid metabolism, protein turnover/folding, nucleotide/DNA synthesis, transport, nucleolus function/RNA binding, transcription and splicing, oxidative stress and signal transduction. The identified targets support our current view that myc acts as a master gene for growth control and increases transcription of a large variety of genes.
Schuhmacher
M
M
GSF Research Centre, Institute of Clinical Molecular Biology, Marchioninistrasse 25, D-81377 Munich, Germany.
Kohlhuber
F
F
Hölzel
M
M
Kaiser
C
C
Burtscher
H
H
Jarsch
M
M
Bornkamm
G W
GW
Laux
G
G
Polack
A
A
Weidle
U H
UH
Eick
D
D
D001402:B-Lymphocytes / Q000378:metabolism* / Q000473:pathology; D015152:Blotting, Northern; D002051:Burkitt Lymphoma / Q000235:genetics / Q000473:pathology; D018929:Cell Culture Techniques; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000235:genetics; D018390:Gene Targeting; D016259:Genes, myc / Q000235:genetics*; D006801:Humans; D007700:Kinetics; D020411:Oligonucleotide Array Sequence Analysis; D000090063:Proto-Oncogene Mas; D014158:Transcription, Genetic; D015533:Transcriptional Activation / Q000235:genetics; D014162:Transfection; D014407:Tumor Cells, Cultured