The effect of microfilament inhibitor on the Cryptosporidium infection in vitro.
This study was focused on the effects of microfilament inhibitor, Cytochalasin D (CD) on the invasiveness of sporozoites of Cryptosporidium spp. into the host cells. MDCK and AGS cell lines were used as host cells for C. parvum and C. muris, respectively. When MDCK cells were pretreated with CD for 1 hr before inoculation of the sporozoites, C. parvum infection was significantly inhibited when compared to the control cells. These inhibitory effects of CD on the rate of infection were dose-dependent. In addition, C. muris infection was hampered when AGS cell lines were pretreated with CD. However, the capability of invasiveness of the sporozoites into the host cells was not greatly influenced by the pretreatment of sporozoites with CD before infection. These results suggest that microfilaments of host cells, rather than parasites, play an important role for the invasion of Cryptosporidium spp.
Yu
J R
JR
Department of Parasitology, College of Medicine, Konkuk University, Chungju 380-701, Korea. jaeran.yu@mail.kku.ac.kr
Choi
S D
SD
D008841:Actin Cytoskeleton / Q000187:drug effects* / Q000502:physiology; D000818:Animals; D002478:Cells, Cultured; D003457:Cryptosporidiosis / Q000188:drug therapy* / Q000209:etiology; D003458:Cryptosporidium / Q000472:pathogenicity; D015638:Cytochalasin D / Q000494:pharmacology / Q000627:therapeutic use*; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D006801:Humans
D015638:Cytochalasin D