Developments in the measurement of plasma total homocysteine.
Elevated plasma homocysteine is considered to be a graded risk factor for cardiovascular disease, and for this reason there is great interest in high-performance analytical techniques. Methods have evolved from ion-exchange chromatography to embrace high-performance liquid chromatography with fluorescence or electrochemical detection, immunoassays, gas chromatography-mass spectroscopy and liquid chromatography with tandem mass spectrometry. Immunoassays and high-performance liquid chromatography methods are currently available in kit form, fluorescence polarization immunoassay showing the best performance.
Powers
H J
HJ
The Centre for Human Nutrition, Division of Clinical Sciences, University of Sheffield, Sheffield, UK. h.j.powers@sheffield.ac.uk
Moat
S J
SJ
D002318:Cardiovascular Diseases / Q000097:blood / Q000175:diagnosis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D012307:Risk Factors; D013050:Spectrometry, Fluorescence
D006710:Homocysteine