Stabilisation of Salmonella vaccine vectors by the induction of trehalose biosynthesis.
The growth of an aroA mutant of Salmonella typhimurium (SL3261) in minimal medium containing 0.5 M NaCl resulted in the intracellular accumulation of 2.2 micromol trehalose/mg total protein. The vacuum drying of these bacteria in the presence of trehalose allowed the recovery of 35% of the viable cells that were present before drying. In contrast, bacteria cultured in control medium accumulated 0.4 micromol trehalose/mg total protein and only 5% of the viable cells were recovered after vacuum drying with trehalose. Similar results were obtained when S. typhimurium SL3261, expressing the vaccine antigen (F1-antigen) of Yersinia pestis, was cultured in minimal medium with or without 0.5 M NaCl and dried in the presence of trehalose. Although these results indicate the potential for trehalose stabilisation of vaccine strains of S. typhimiurium, growth in minimal medium containing 0.5 M NaCl resulted in the loss of invasion competence of the bacteria.
Bullifent
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HL
Defence Evaluation and Research Agency, CBD Porton Down, Wiltshire SP4 0JQ, Salisbury, UK.
Dhaliwal
K
K
Howells
A M
AM
Goan
K
K
Griffin
K
K
Lindsay
C D
CD
Tunnacliffe
A
A
Titball
R W
RW
D000818:Animals; D002460:Cell Line; D005260:Female; D005964:Glucosyltransferases / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics; D022562:Salmonella Vaccines / Q000737:chemistry / Q000276:immunology*; D012486:Salmonella typhimurium / Q000254:growth & development / Q000276:immunology*; D014199:Trehalose / Q000096:biosynthesis*
D022562:Salmonella Vaccines; D014199:Trehalose; D005964:Glucosyltransferases; C023180:trehalose-6-phosphate synthase; C023178:trehalose-phosphatase; D010744:Phosphoric Monoester Hydrolases