Caenorhabditis elegans as an alternative animal species.
Caenorhabditis elegans has proven useful in toxicity testing of known toxicants, but its potential for assessing the toxicity of new pharmaceuticals is relatively unexplored. In this study the procedures used in aquatic testing of toxicants were modified to permit testing of small amounts (<40 mg) of gadolinium-based magnetic resonance imaging (MRI) compounds. Five blinded compounds were tested. The toxicity of these compounds determined using C. elegans was compared to existing mammalian test system data (minimum lethal dose [MLD] values for mice). Four of five compounds tested had the same relative sensitivity with C. elegans as with the mouse test system. Testing with C. elegans is efficient and could markedly reduce the cost of screening potentially useful compounds.
Williams
P L
PL
Department of Environmental Health Science, University of Georgia, Athens 30602-2102, USA. pwilliam@arches.uga.edu
Anderson
G L
GL
Johnstone
J L
JL
Nunn
A D
AD
Tweedle
M F
MF
Wedeking
P
P
D000826:Animal Testing Alternatives; D000818:Animals; D017173:Caenorhabditis elegans; D003287:Contrast Media / Q000633:toxicity; D003363:Cost Control; D004345:Drug Industry; D008279:Magnetic Resonance Imaging; D051379:Mice; D012680:Sensitivity and Specificity; D018675:Toxicity Tests / Q000379:methods
D003287:Contrast Media