Overexpression of CuZnSOD gene suppresses the growth of hepatocellular cancer cell line HepG2.
OBJECTIVE
To explore the inhibiting effect of superoxide dismutase (SOD) on the growth of hepatocellular cancer cell line HepG2.


METHODS
By gene transfer technique, hepatocellular cancer cells (HepG2) were transfected with a retroviral vector containing human CuZnSOD cDNA. The elevated SOD gene expression of the transfected cells was compared with the parental and neo control cells.


RESULTS
Compared to the control cells, cancer cells transfected with SOD gene showed an inhibited cell growth, a reduced number of cells in S phase and decreased clone forming ability in soft agar, as well as a smaller tumor size formed in nude mice.


CONCLUSIONS
The overexpression of CuZnSOD gene could, to certain extent, suppress the cell growth of hepatocellular cancer cell line HepG2.
Bai
J
J
Department of Surgery, People's Hospital, Beijing Medical University, Beijing 100044, China.
Zhu
X
X
Zheng
X
X
Wu
Y
Y
D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000473:pathology*; D002455:Cell Division; D015870:Gene Expression; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology*; D012190:Retroviridae; D013482:Superoxide Dismutase / Q000096:biosynthesis / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured
D013482:Superoxide Dismutase