title: Immune response to Plasmodium falciparum liver stage antigen-1: geographical variations within Central Africa and their relationship with protection from clinical malaria.
abstract: Two populations of schoolchildren from Gabon and Cameroon were tested in 1995 for their immunological reactivity to synthetic peptides (LSA-Rep, LSA-J and LSA-CTL) from Plasmodium falciparum liver stage antigen-1 (LSA-1). The prevalence and levels of both cellular (lymphocyte proliferation, tumour necrosis factor alpha (TNF alpha), interferon gamma (IFN gamma), and interleukin-10 (IL-10)) and humoral (immunoglobulin G) responses were determined. Protection from clinical malaria, determined after a prospective 1 year study in both sites, was associated with elevated proliferative responses to LSA-Rep and LSA-CTL in the Gabonese children, as well as with higher antibody levels to both schizont extract and LSA-Rep. The prevalence of peptide-stimulated TNF-alpha secretion was higher in the Cameroonian group, but higher levels of antibodies to LSA-Rep and LSA-J were found in the Gabonese children. The immunological differences observed between children in the 2 study sites are discussed in the context of both epidemiological and individual host factors.
authors.affiliation: Centre International de Recherches Médicales de Franceville (CIRMF), Gabon. migotnabias@cirmf.sci.ga
authors.forename: F
authors.initials: F
authors.lastname: Migot-Nabias
authors.forename: P
authors.initials: P
authors.lastname: Deloron
authors.forename: P
authors.initials: P
authors.lastname: Ringwald
authors.forename: B
authors.initials: B
authors.lastname: Dubois
authors.forename: J
authors.initials: J
authors.lastname: Mayombo
authors.forename: T N
authors.initials: TN
authors.lastname: Minh
authors.forename: N
authors.initials: N
authors.lastname: Fievet
authors.forename: P
authors.initials: P
authors.lastname: Millet
authors.forename: A
authors.initials: A
authors.lastname: Luty
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000953:Antigens, Protozoan / Q000276:immunology*; D002163:Cameroon; D002648:Child; D015331:Cohort Studies; D016207:Cytokines / Q000276:immunology; D005260:Female; D005681:Gabon; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008109:Liver Diseases, Parasitic / Q000276:immunology*; D008213:Lymphocyte Activation; D016778:Malaria, Falciparum / Q000276:immunology*; D008297:Male; D010963:Plasmodium falciparum / Q000276:immunology*
chemical_list: D000953:Antigens, Protozoan; D016207:Cytokines