title: Carrageenan gel and aberrant crypt foci in the colon of conventional and human flora-associated rats.
abstract: Carrageenans (CAR) are sulfated polymers from seaweed used as gelling agents in foods. Chemical carcinogen induction of tumors in the colon of rats is enhanced by CAR. We speculated that gut microflora is involved in this effect. We thus studied the initiating and promoting effects of undegraded CAR-kappa (345,000 mol wt) in conventional rats and in germ-free rats associated with a human fecal flora. The initiating effect of CAR was studied by scoring aberrant crypt foci (ACF) in the colon of Fischer 344 rats given CAR (10% in water). The promoting effect of CAR was studied by comparing the multiplicity of ACF (number of crypts/focus) in rats receiving pure water or CAR (0.25% and 2.5% in water) for 100 days, starting 7 days after azoxymethane initiation (1 dose of 20 mg/kg i.p.). Duplicate studies were conducted in conventional rats and in human flora-associated rats maintained in isolators. Results show that CAR did not initiate ACF. In conventional rats, the 2.5% CAR gel promoted the growth of ACF: 2.98 +/- 0.29 and 3.44 +/- 0.48 crypts/ACF in control and treated rats, respectively (p < 0.02). The 0.25% CAR gel did not promote ACF. CAR can thus enhance intestinal tumors in this rat model, but only at a high dose level. In contrast, we did not observe any promoting effect of the administration of the 2.5% CAR gel in human flora-associated rats: 2.81 +/- 0.18 and 2.78 +/- 0.38 crypts/ACF in control and treated rats, respectively (p = 0.80). The specific microflora of rats, but not the human gut flora, might be involved in colon tumor enhancement by CAR.
authors.affiliation: Laboratoire Sécurité des Aliments, Institut National de la Recherche Agronomique, Ecole Nationale Vétérinaire, 31076 Toulouse, France.
authors.forename: S
authors.initials: S
authors.lastname: Taché
authors.forename: G
authors.initials: G
authors.lastname: Peiffer
authors.forename: A S
authors.initials: AS
authors.lastname: Millet
authors.forename: D E
authors.initials: DE
authors.lastname: Corpet
mesh_terms: D000818:Animals; D001397:Azoxymethane; D002273:Carcinogens; D002351:Carrageenan / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D003106:Colon / Q000187:drug effects* / Q000382:microbiology / Q000473:pathology; D003110:Colonic Neoplasms / Q000139:chemically induced* / Q000382:microbiology / Q000517:prevention & control; D004305:Dose-Response Relationship, Drug; D005243:Feces / Q000737:chemistry; D005260:Female; D005782:Gels; D005856:Germ-Free Life; D008970:Molecular Weight; D011230:Precancerous Conditions / Q000139:chemically induced* / Q000382:microbiology; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D002273:Carcinogens; D005782:Gels; D002351:Carrageenan; D001397:Azoxymethane