[Features of interactions of p68 anchorosomal protein with the nuclear envelope in the cell cycle].
Chromatin associated with the nuclear envelope appears in the interphase nuclei as a layer of anchorosomes, granules 20-25 nm in diameter. The fraction of chromatin directly associated with the nuclear envelope is resistant to decondensation, shows a low level of DNA methylation, and contains specific acid-soluble proteins. However, mechanisms underlying the interaction of chromatin with the nuclear envelope are not fully understood. Specifically, it is not known whether anchorosomes are permanent structures or if they undergo reversible disassembly during mitosis, when contacts between chromatin and the nuclear envelope are destroyed. We obtained immune serum recognizing a 68 kDa protein from the nuclear envelopes fraction and studied the localization of this protein in interphase and mitotic cells. We show that this protein present in the NE/anchorosomal fraction does not remain bound with chromosomes during mitosis. It dissociates from chromosomes at the beginning of the prophase and then can be identified again at the periphery of the newly forming nuclei in the telophase.
Kurchashova
S Iu
SIu
Belozerskii Institute of Molecular Biology and Bioorganic Chemistry, Moscow State University, Vorob'evy gory, Moscow, 119899 Russia.
Prusov
A N
AN
Gulak
P V
PV
Kireev
I I
II
Poliakov
V Iu
VIu
D016475:3T3 Cells; D000818:Animals; D002453:Cell Cycle; D002460:Cell Line; D007106:Immune Sera; D051379:Mice; D008854:Microscopy, Electron; D009685:Nuclear Envelope / Q000378:metabolism* / Q000648:ultrastructure; D009687:Nuclear Proteins / Q000378:metabolism*; D011485:Protein Binding; D013552:Swine
D007106:Immune Sera; D009687:Nuclear Proteins