title: [Quantitative vascular analysis in the marginal periodontium using PECAM-1].
abstract: BACKGROUND
  Systemic sclerosis (SSc) is a generalized disorder of the interstitial tissues and vasculature with distinct abnormalities in three systems, immune and autoimmune, vascular and microvascular, and mesenchymal extracellular matrix (ECM), that lead to exuberant fibrosis. The aim of this study was to compare the number of blood vessel profiles in the marginal gingiva between SSc patients and patients with periodontitis but without SSc by using biopsies.
  
  
  METHODS
  Marginal gingiva and gingival papilla were obtained from 13 scleroderma patients and 8 patients with periodontitis after routine tooth extraction and gingival curettage. On the histological sections, immunohistochemical investigations were performed using the avidin-biotin complex method (ABC) and the monoclonal antibody CD 31/Clone JC70A (platelet endothelial cell adhesion molecule-1). Blood vessels were identified by light microscopy (original magnification x400) and counted within 0.3615 mm2. Medians of blood vessel profiles were compared by Mann-Whitney U-test.
  
  
  RESULTS
  There is no statistical difference between the median of blood vessel profiles in the marginal gingiva of SSc patients as compared to the median of blood vessel profiles in the marginal gingiva of patients with periodontitis (P = 0.665). We did not discover avascular areas in the subepithelial connective tissue.
  
  
  CONCLUSIONS
  The pathological changes in the microvasculature in the dermis of SSc patients are not transferable to the marginal periodont, as intraindividual histological examinations of dermis and oral mucosa in relation to the degree of the disease are not available yet.
authors.affiliation: Abteilung für Zahnärztliche Chirurgie, Fakultät für Zahn-, Mund- und Kieferheilkunde, Universität Witten/Herdecke, Alfred-Herrhausen-Strasse 50, 58448 Witten.
authors.forename: J
authors.initials: J
authors.lastname: Jackowski
authors.forename: F
authors.initials: F
authors.lastname: Straube
authors.forename: S
authors.initials: S
authors.lastname: Philippou
mesh_terms: D000328:Adult; D000368:Aged; D003774:Dental Plaque Index; D005109:Extracellular Matrix / Q000473:pathology; D005260:Female; D005881:Gingiva / Q000098:blood supply; D006801:Humans; D008297:Male; D008833:Microcirculation / Q000473:pathology; D008875:Middle Aged; D010512:Periodontal Index; D010519:Periodontium / Q000098:blood supply*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000032:analysis*; D012595:Scleroderma, Systemic / Q000473:pathology*
chemical_list: D019408:Platelet Endothelial Cell Adhesion Molecule-1