title: Genomic exploration of the hemiascomycetous yeasts: 9. Saccharomyces kluyveri.
abstract: The genome of Saccharomyces kluyveri was explored through 2528 random sequence tags with an average length of 981 bp. The complete nuclear ribosomal DNA unit was found to be 8656 bp in length. Sequences homologous to retroelements of the gypsy and copia types were identified as well as numerous solo long terminal repeats. We identified at least 1406 genes homologous to Saccharomyces cerevisiae open reading frames, with on average 58.1% and 72.4% amino acid identity and similarity, respectively. In addition, by comparison with completely sequenced genomes and the SwissProt database, we found 27 novel S. kluyveri genes. Most of these genes belong to pathways or have functions absent from S. cerevisiae, such as the catabolic pathway of purines or pyrimidines, melibiose fermentation, sorbitol utilization, or degradation of pollutants. The sequences are deposited in EMBL under the accession numbers AL404849-AL407376.
authors.affiliation: Collection de Levures d'Intérêt Biotechnologie, Laboratoire de Génétique Moléculaire et Cellulaire, INRA UMR216, CNRS URA1925, INA-PG, Thiverval-Grignon, France. ncecile@grignon.inra.fr
authors.forename: C
authors.initials: C
authors.lastname: Neuvéglise
authors.forename: E
authors.initials: E
authors.lastname: Bon
authors.forename: A
authors.initials: A
authors.lastname: Lépingle
authors.forename: P
authors.initials: P
authors.lastname: Wincker
authors.forename: F
authors.initials: F
authors.lastname: Artiguenave
authors.forename: C
authors.initials: C
authors.lastname: Gaillardin
authors.forename: S
authors.initials: S
authors.lastname: Casarégola
mesh_terms: D001203:Ascomycota / Q000235:genetics; D005656:Fungal Proteins / Q000145:classification / Q000235:genetics; D016681:Genome, Fungal; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D012343:RNA, Transfer / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D012440:Saccharomyces / Q000235:genetics*
chemical_list: D005656:Fungal Proteins; D012343:RNA, Transfer