Expression, extracellular secretion, and immunogenicity of the Plasmodium falciparum sporozoite surface protein 2 in Salmonella vaccine strains.
Deleting transmembrane alpha-helix motifs from Plasmodium falciparum sporozoite surface protein (SSP-2) allowed its secretion from Salmonella enterica serovar Typhimurium SL3261 and S. enterica serovar Typhi CVD 908-htrA by the Hly type I secretion system. In mice immunized intranasally, serovar Typhimurium constructs secreting SSP-2 stimulated greater gamma interferon splenocyte responses than did nonsecreting constructs (P = 0.04).
Gómez-Duarte
O G
OG
Center for Vaccine Development, Department of Medicine, School of Medicine, University of Maryland, Baltimore, Maryland 21201, USA.
Pasetti
M F
MF
Santiago
A
A
Sztein
M B
MB
Hoffman
S L
SL
Levine
M M
MM
D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D005260:Female; D007114:Immunization; D008213:Lymphocyte Activation; D017780:Malaria Vaccines / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D010963:Plasmodium falciparum / Q000276:immunology*; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D012475:Salmonella / Q000235:genetics*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology
D000953:Antigens, Protozoan; D017780:Malaria Vaccines; D015800:Protozoan Proteins; D019444:Vaccines, DNA; C076139:sporozoite-surface protein 2, Plasmodium yoelli