Molecular cloning and characterization of a microsatellite locus found in an RAPD marker of a spider mite, Panonychus citri (Acari: Tetranychidae).
Genetic markers were searched using PCR with 40 kinds of decanucleotide primers to investigate DNA polymorphism in Panonychus citri. A region consisting of a variable number of CT tandem repeats (microsatellite) was found in a fragment amplified with the OPB10 primer. The microsatellite differed in size by ca. 100bp among several P. citri populations screened and was derived from at least seven alleles. This region was characteristic of P. mori and P. osmanthi, but was lacking in P. ulmi. The flanking regions were highly conserved among these species.
Osakabe
M
M
Department of Plant Protection, National Agriculture Research Center, Tsukuba, Ibaraki, Japan. mhosaka@narc.affrc.go.jp
Hinomoto
N
N
Toda
S
S
Komazaki
S
S
Goka
K
K
D000483:Alleles; D000818:Animals; D003001:Cloning, Molecular; D014644:Genetic Variation; D018895:Microsatellite Repeats; D018598:Minisatellite Repeats; D008925:Mites / Q000235:genetics*; D008969:Molecular Sequence Data; D019105:Random Amplified Polymorphic DNA Technique