Distinct proteins interacting with the glucocorticoid receptor.
The glucocorticoid receptor (GR) is a hormone-dependent transcription factor that modulates gene transcription by binding to its response element, interacting with other transcription factors, contacting the basal transcription machinery and by interacting with several intermediary proteins, such as coactivators. Here we discuss the most prominent examples for interacting transcription factors, interacting proteins that belong to the basal transcription apparatus and intermediary factors. Furthermore, we describe a new class of GR-interacting proteins (GRIPs) which have been identified by a blotting technique, using a radioactively labeled GR/GRE complex as a probe to identify proteins that interact with the activated GR. Two of these GRIPs, termed GRIP170 and GRIP 120, which were identified by biochemical purification and microsequencing and have turned out to play a role in modulating GR-mediated transcription, are discussed in detail.
Schulz
M
M
Institut für Genetik, Justus-Liebig-Universität, Heinrich-Buff-Ring 58-62 D-35392 Giessen, Germany.
Zhang
R
R
Eggert
H
H
Boeke
J
J
Neeck
G
G
Eggert
M
M
Renkawitz
R
R
D000818:Animals; D001327:Autoimmune Diseases / Q000235:genetics / Q000628:therapy; D015316:Genetic Therapy; D006801:Humans; D051958:Nuclear Receptor Coactivator 2; D011965:Receptors, Glucocorticoid / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
D051958:Nuclear Receptor Coactivator 2; D011965:Receptors, Glucocorticoid; D014157:Transcription Factors