Expression of nociceptin/OFQ receptor and prepro-nociceptin/OFQ in lymphoid tissues.
This study was undertaken to examine the presence of functional nociceptin/orphanin FQ (N/OFQ) receptors in the immune system. Receptor mRNA signals were detected by RT-PCR in porcine thymus, lymph nodes, spleen and freshly-isolated splenocytes; the distribution of prepro-nociceptin/-orphanin FQ (PP-N/-OFQ) mRNA was similar, with the exception of lymph nodes. However, specific [(3)H]nociceptin binding sites were not detected in rat or porcine lymphoid tissues, and 0.1-100 nM nociceptin had no effect on forskolin-stimulated cyclic AMP concentrations in porcine splenocytes. Thus, it appears that nociceptin/orphanin FQ receptor mRNA, but not a functional receptor protein is expressed in the immune system.
Pampusch
M S
MS
Department of Veterinary PathoBiology, University of Minnesota, St. Paul, MN, USA.
Serie
J R
JR
Osinski
M A
MA
Seybold
V S
VS
Murtaugh
M P
MP
Brown
D R
DR
D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000378:metabolism; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D008198:Lymph Nodes / Q000378:metabolism*; D008297:Male; D011498:Protein Precursors / Q000096:biosynthesis*; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011957:Receptors, Opioid / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000166:cytology / Q000378:metabolism*; D013552:Swine; D013950:Thymus Gland / Q000378:metabolism*; D013997:Time Factors; D014018:Tissue Distribution; D000094942:Nociceptin Receptor
D018076:DNA, Complementary; D011498:Protein Precursors; D012333:RNA, Messenger; D011957:Receptors, Opioid; C121527:prepronociceptin; D005576:Colforsin; D012313:RNA; D000242:Cyclic AMP; D000094942:Nociceptin Receptor