In vivo selection of T-cell receptor junctional region sequences by HLA-A2 human T-cell lymphotropic virus type 1 Tax11-19 peptide complexes.
Using HLA-peptide tetrameric complexes, we isolated human T-cell lymphotrophic virus type 1 Tax peptide-specific CD8(+) T cells ex vivo. Antigen-specific amino acid motifs were identified in the T-cell receptor Vbeta CDR3 region of clonally expanded CD8(+) T cells. This result directly confirms the importance of the CDR3 region in determining the antigen specificity in vivo.
Saito
M
M
Departments of Immunology, Imperial College School of Medicine, St. Mary's Campus, London W2 1PG, United Kingdom.
Taylor
G P
GP
Saito
A
A
Furukawa
Y
Y
Usuku
K
K
Weber
J N
JN
Osame
M
M
Bangham
C R
CR
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D022801:Complementarity Determining Regions / Q000235:genetics; D016356:Gene Products, tax / Q000276:immunology / Q000378:metabolism*; D015789:HLA-A2 Antigen / Q000276:immunology / Q000378:metabolism*; D015490:HTLV-I Infections / Q000276:immunology / Q000821:virology; D015368:Human T-lymphotropic virus 1 / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D022801:Complementarity Determining Regions; D016356:Gene Products, tax; D015789:HLA-A2 Antigen; D016693:Receptors, Antigen, T-Cell, alpha-beta