title: The genetic ablation of cyclooxygenase 2 prevents the development of autoimmune arthritis.
abstract: OBJECTIVE
  To determine the effects of cyclooxygenase 1 (COX-1) and COX-2 gene deletion on collagen-induced arthritis (CIA).
  
  
  METHODS
  Mice that were susceptible to CIA but lacked either the COX-1 or the COX-2 gene were immunized with type II collagen (CII), and the incidence and severity of arthritis were compared with findings in wild-type animals, by clinical and histologic examination. The immune response was assessed by measuring total CII IgG, IgG1, and IgG2 antibody production in sera from immunized mice. The passive transfer of arthritis, accomplished using anti-CII monoclonal antibodies, was tested in wild-type and COX-deficient (-/-) mice. Splenocytes cultured from CII-immunized wild-type and COX-/- mice were challenged with bovine alpha1(II), and cytokine production was assessed.
  
  
  RESULTS
  COX-2 gene deletion reduced the incidence and severity of CIA compared with findings in wild-type and COX-1-/- mice. Histologic examination of joints after the onset of clinical arthritis revealed cartilage erosions, proliferation of the synovial lining, and inflammatory cell infiltration in wild-type and COX-1-/- mice, but not in COX-2-/- mice. COX-2-/- mice exhibited reduced anti-CII IgG antibody levels, indicating a decreased immune response. However, cytokine production by spleen cells from immunized mice indicated no cytokine deficiencies in COX-2-/- mice compared with wild-type or COX-1-/- mice. More important, arthritis could not be passively transferred to naive COX-2-/- mice, indicating a requirement for COX-2 in the pathogenesis of arthritis, independent of the immune response.
  
  
  CONCLUSIONS
  COX-2-/- mice exhibit at least 2 defects resulting in down-modulation of the development of CIA: a reduced immune response to CII demonstrated by a markedly reduced antibody titer, and an "inflammatory" defect reflected by the inability to passively transfer arthritis to COX-2-/- mice.
authors.affiliation: University of Tennessee, Memphis, USA.
authors.forename: L K
authors.initials: LK
authors.lastname: Myers
authors.forename: A H
authors.initials: AH
authors.lastname: Kang
authors.forename: A E
authors.initials: AE
authors.lastname: Postlethwaite
authors.forename: E F
authors.initials: EF
authors.lastname: Rosloniec
authors.forename: S G
authors.initials: SG
authors.lastname: Morham
authors.forename: B V
authors.initials: BV
authors.lastname: Shlopov
authors.forename: S
authors.initials: S
authors.lastname: Goorha
authors.forename: L R
authors.initials: LR
authors.lastname: Ballou
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000917:Antibody Formation; D001168:Arthritis / Q000139:chemically induced / Q000276:immunology*; D001327:Autoimmune Diseases / Q000517:prevention & control*; D003094:Collagen / Q000276:immunology; D051546:Cyclooxygenase 2; D016207:Cytokines / Q000502:physiology; D017353:Gene Deletion; D007114:Immunization; D007116:Immunization, Passive; D007527:Isoenzymes / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics*; D013154:Spleen / Q000737:chemistry / Q000166:cytology
chemical_list: D000911:Antibodies, Monoclonal; D016207:Cytokines; D007527:Isoenzymes; D003094:Collagen; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases