Presence of immunoreactive adrenomedullin in human and bovine milk.
We examined by radioimmunoassay the presence of immunoreactive adrenomedullin (ir-AM) in human and bovine milk. Milk samples displaced (125)I-AM from the AM-antiserum in parallel to the standard curve. RP-HPLC revealed a main immunoreactive peak eluting as synthetic AM. Concentrations in human milk ranged between 140 and 404 pg/mL. In cow, the levels of AM were 73.5 +/- 3.8 pg/mL. Bovine milk products had AM levels similar to those found in fresh bovine milk. Human milk had growth promoting activity on the human intestinal cell line Int-407 that could be partially blocked with an anti-AM antibody.
Pio
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R
Department of Cell and Cancer Biology, National Cancer Institute, NIH, Building 10 Room 12N226, 9000 Rockville Pike, Bethesda, MD 20892, USA. pior@mail.nih.gov
Martínez
A
A
Elsasser
T H
TH
Cuttitta
F
F
D053607:Adrenomedullin; D000818:Animals; D001667:Binding, Competitive; D002417:Cattle; D002455:Cell Division; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007422:Intestines / Q000166:cytology; D008892:Milk / Q000737:chemistry / Q000378:metabolism; D010455:Peptides / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism*; D011863:Radioimmunoassay; D013997:Time Factors
D010455:Peptides; D053607:Adrenomedullin