title: A novel monoclonal antibody specific for the N-terminal end of GAD65.
abstract: We describe a novel monoclonal antibody raised towards the N-terminus of the 65 kDa isoform of glutamate decarboxylase (GAD65). This N-GAD65 mAb is highly specific for GAD65 and can be used in a wide range of applications such as Western blot analysis, immunoprecipitation and immunocytochemistry. Full-length cDNAs coding for N-GAD65 mAb heavy and light chains were cloned and characterized by nucleotide sequence analysis. Both heavy and light chain-specific cDNAs are functional and are members of mouse heavy chain subgroup IgG1 and kappa chain group 2, respectively. Comparing the N-GAD65 mAb gene sequences with GenBank shows that the cDNAs were not reported previously.
authors.affiliation: Robert H. Williams Laboratory, Department of Medicine, Box 357710, University of Washington, Seattle, 98105-7710, USA. champe@u.washington.edu
authors.forename: C S
authors.initials: CS
authors.lastname: Hampe
authors.forename: P
authors.initials: P
authors.lastname: Lundgren
authors.forename: T L
authors.initials: TL
authors.lastname: Daniels
authors.forename: L P
authors.initials: LP
authors.lastname: Hammerle
authors.forename: S M
authors.initials: SM
authors.lastname: Marcovina
authors.forename: A
authors.initials: A
authors.lastname: Lernmark
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics* / Q000276:immunology*; D000918:Antibody Specificity; D001323:Autoantibodies / Q000276:immunology; D015153:Blotting, Western; D018076:DNA, Complementary; D003922:Diabetes Mellitus, Type 1 / Q000201:enzymology / Q000276:immunology; D005968:Glutamate Decarboxylase / Q000737:chemistry / Q000276:immunology*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics / Q000276:immunology; D007147:Immunoglobulin Light Chains / Q000235:genetics / Q000276:immunology; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000737:chemistry / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D011863:Radioimmunoassay; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D018076:DNA, Complementary; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007527:Isoenzymes; D005968:Glutamate Decarboxylase; C401141:glutamate decarboxylase 2