title: Immunological and PCR analyses for Borna disease virus in psychiatric patients and blood donors in Japan.
abstract: The involvement of Borna disease virus (BDV) in psychiatric diseases in humans remains controversial. T-cell memory response and seroprevalence of BDV in patients with psychiatric disorders and blood donors in Japan were evaluated collectively by Western blot (WB) analysis with inhibition test, electrochemiluminescence immunoassay, immunofluorescence assay, and T-cell proliferative response as well as detection of BDV p24 RNA in peripheral blood mononuclear cells (PBMCs). Positive proliferative responses to both BDV p40 and p24 proteins were detected in 9% of patients with mood disorders (4 of 45), 4% of schizophrenic patients (2 of 45), and 2% of blood donors (1 of 45). By WB analysis, the antibody to BDV p40 was detected only in 2% of patients with mood disorders (1 of 45). The BDV p24 antibody was detected in 2% of patients with mood disorders (1 of 45) and 9% of schizophrenic patients. (4 of 45) No plasma reacted with both BDV proteins. The finding of a lower seroprevalence than previously reported suggests the presence of false-positive cases in the previous report. BDV RNA was detected only in 2% of patients with mood disorders (1 of 45). In these three serological assays, T-cell responses, and PCR analysis, there was no significant difference in the prevalence among the three groups. However, we found three psychiatric patients who were positive for both BDV antibodies and T-cell proliferative responses and one patient who was positive for BDV RNA in PBMCs. These findings suggest the usefulness of the proliferative T-cell response and that certain individuals are infected with BDV or a BDV-related virus.
authors.affiliation: Department of Microbiology, Fukushima Medical University, Fukushima-shi, Fukushima 960-1295, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Fukuda
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
authors.forename: Y
authors.initials: Y
authors.lastname: Iwata
authors.forename: N
authors.initials: N
authors.lastname: Mori
authors.forename: K
authors.initials: K
authors.lastname: Gonda
authors.forename: T
authors.initials: T
authors.lastname: Ogawa
authors.forename: K
authors.initials: K
authors.lastname: Osonoe
authors.forename: M
authors.initials: M
authors.lastname: Sato
authors.forename: S
authors.initials: S
authors.lastname: Ogata
authors.forename: T
authors.initials: T
authors.lastname: Horimoto
authors.forename: T
authors.initials: T
authors.lastname: Sawada
authors.forename: M
authors.initials: M
authors.lastname: Tashiro
authors.forename: K
authors.initials: K
authors.lastname: Yamaguchi
authors.forename: S
authors.initials: S
authors.lastname: Niwa
authors.forename: S
authors.initials: S
authors.lastname: Shigeta
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000914:Antibodies, Viral / Q000097:blood; D001782:Blood Donors; D015153:Blotting, Western / Q000379:methods; D001890:Borna Disease / Q000175:diagnosis*; D001891:Borna disease virus / Q000302:isolation & purification*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007118:Immunoassay; D007156:Immunologic Memory; D007564:Japan; D008163:Luminescent Measurements; D008213:Lymphocyte Activation; D008297:Male; D001523:Mental Disorders / Q000150:complications / Q000821:virology*; D008875:Middle Aged; D019964:Mood Disorders / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods; D012367:RNA, Viral / Q000097:blood; D012559:Schizophrenia / Q000821:virology; D013601:T-Lymphocytes / Q000276:immunology / Q000821:virology
chemical_list: D000914:Antibodies, Viral; D012367:RNA, Viral