Quantification of human cytomegalovirus DNA by real-time PCR.
A quantitative real-time PCR assay was developed to measure human cytomegalovirus (HCMV) DNA load in peripheral blood leukocytes (PBLs). The HCMV DNA load in PBLs was normalized by means of the quantification of a cellular gene (albumin). The results of the real-time PCR assay correlated with those of the HCMV pp65-antigenemia assay (P < 0.0001).
Gault
E
E
Laboratoire de Virologie, Hôpital Armand Trousseau, Paris, France.
Michel
Y
Y
Dehée
A
A
Belabani
C
C
Nicolas
J C
JC
Garbarg-Chenon
A
A
D000956:Antigens, Viral / Q000097:blood; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000097:blood*; D006801:Humans; D007962:Leukocytes / Q000821:virology*; D010750:Phosphoproteins / Q000097:blood; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012709:Serum Albumin / Q000235:genetics; D019562:Viral Load; D014763:Viral Matrix Proteins / Q000097:blood
D000956:Antigens, Viral; D004279:DNA, Viral; D010750:Phosphoproteins; D012709:Serum Albumin; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa