title: The Pseudomonas fluorescens transcription activator AdnA is required for adhesion and motility.
abstract: The locations of two mutations that prevent adhesion of Pseudomonas fluorescens Pf0-1 to sand columns and seeds (adn, adhesion) were identified. Both lie in a single gene showing homology to the NtrC/NifA family of transcription activators. The predicted 55 kDa protein encoded by adnA is most closely related to activators involved in expression of flagellar proteins, consistent with the lack of flagella in adnA strains. Constitutive adnA expression restored motility and adhesion to an adnA strain, demonstrating that the observed phenotypes are due to lack of AdnA and not a consequence of other mutations or polar effects of mutations in adnA on other genes.
authors.affiliation: Center for Adaptation Genetics and Drug Resistance, and Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111, USA1.
authors.forename: Paul
authors.initials: P
authors.lastname: Casaz
authors.affiliation: Center for Adaptation Genetics and Drug Resistance, and Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111, USA1.
authors.forename: Anne
authors.initials: A
authors.lastname: Happel
authors.affiliation: Center for Adaptation Genetics and Drug Resistance, and Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111, USA1.
authors.forename: Joann
authors.initials: J
authors.lastname: Keithan
authors.affiliation: Center for Adaptation Genetics and Drug Resistance, and Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111, USA1.
authors.forename: Dorothy L
authors.initials: DL
authors.lastname: Read
authors.affiliation: Center for Adaptation Genetics and Drug Resistance, and Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111, USA1.
authors.forename: Steven R
authors.initials: SR
authors.lastname: Strain
authors.affiliation: Center for Adaptation Genetics and Drug Resistance, and Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111, USA1.
authors.forename: Stuart B
authors.initials: SB
authors.lastname: Levy
mesh_terms: D000595:Amino Acid Sequence; D001422:Bacterial Adhesion; D001426:Bacterial Proteins; D003001:Cloning, Molecular; D003360:Cosmids / Q000235:genetics; D004251:DNA Transposable Elements; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009068:Movement; D016254:Mutagenesis, Insertional; D011551:Pseudomonas fluorescens / Q000235:genetics / Q000502:physiology*; D017422:Sequence Analysis, DNA; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
chemical_list: C433520:AdnA protein, Pseudomonas fluorescens; D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D015534:Trans-Activators