title: Magnetic bead capture eliminates PCR inhibitors in samples collected from the airborne environment, permitting detection of Pneumocystis carinii DNA.
abstract: PCR detection methods are useful in studies of organisms not amenable to culture. Inhibitors in environmental samples can interfere with such assays. We describe a magnetic bead DNA capture protocol that removes inhibitors from outdoor air samples, maintaining the sensitivity of a 16S Pneumocystis carinii mitochondrial rRNA gene-based PCR.
authors.affiliation: Division of Geographic Medicine and Department of Environmental Health Sciences, University of Alabama at Birmingham, 35294-2170, USA.
authors.forename: N
authors.initials: N
authors.lastname: Maher
authors.forename: H K
authors.initials: HK
authors.lastname: Dillon
authors.forename: S H
authors.initials: SH
authors.lastname: Vermund
authors.forename: T R
authors.initials: TR
authors.lastname: Unnasch
mesh_terms: D000391:Air Microbiology; D004271:DNA, Fungal / Q000032:analysis*; D004784:Environmental Monitoring / Q000379:methods*; D005374:Filtration; D008280:Magnetics; D011010:Pneumocystis / Q000235:genetics / Q000302:isolation & purification*; D011076:Polycarboxylate Cement; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D004271:DNA, Fungal; D011076:Polycarboxylate Cement; C005506:polycarbonate