SigE is a chaperone for the Salmonella enterica serovar Typhimurium invasion protein SigD.
SigD is translocated into eucaryotic cells by a type III secretion system. In this work, evidence that the putative chaperone SigE directly interacts with SigD is presented. A bacterial two-hybrid system demonstrated that SigE can interact with itself and SigD. In addition, SigD was specifically copurified with SigE-His(6) on a nickel column.
Darwin
K H
KH
Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Robinson
L S
LS
Miller
V L
VL
D001426:Bacterial Proteins / Q000378:metabolism*; D005408:Flagellin / Q000378:metabolism*; D018832:Molecular Chaperones / Q000378:metabolism*; D011485:Protein Binding; D012486:Salmonella typhimurium / Q000378:metabolism* / Q000472:pathogenicity; D012808:Sigma Factor / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D001426:Bacterial Proteins; D018832:Molecular Chaperones; D012808:Sigma Factor; C111259:sigE protein, Bacteria; D005408:Flagellin; C416532:flaB flagellin