Two types of putative nuclear factors that physically interact with histidine-containing phosphotransfer (Hpt) domains, signaling mediators in His-to-Asp phosphorelay, in Arabidopsis thaliana.
The higher plant, Arabidopsis thaliana, has a large number of genes, each of which encodes a component of His-to-Asp phosphorelay signal transduction systems. One type of such signal transducers are the histidine-containing phosphotransmitters (termed AHPs), which presumably mediate His-to-Asp phosphorelay. Here we attempted to isolate a factor or factors that interact with AHP1, AHP2 and AHP3 by means of a yeast two-hybrid system. This allowed us to identify two types of nuclear-localizing proteins. They are the members of the type-B family of response regulators (specifically, ARR1, APP2 and ARR10), and a novel protein named TCP10. The binding of ARR1 to AHP2 was also confirmed by in vitro binding assays. Moreover, dephosphorylation of AHP2 was observed in a manner dependent on ARR in vitro. A subset of AHPs appeared to also interact with a protein that contains a TCP domain, a recently proposed basic helix-loop-helix motif. Because several factors carrying the TCP domain have been implicated in the regulation of growth and development in lateral organs, the binding of TCP10 to this subset of AHPs suggests a possible linkage between the His-to-Asp phosphorelay systems and plant growth regulation.
Suzuki
T
T
School of Agriculture, Bioscience Center, Nagoya University, Chikusa-ku, Nagoya, 464-8601 Japan.
Sakurai
K
K
Ueguchi
C
C
Mizuno
T
T
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism; D001224:Aspartic Acid / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D020134:Catalytic Domain; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics; D017343:Genes, Plant; D005816:Genetic Complementation Test; D006639:Histidine / Q000378:metabolism*; D000071677:Histidine Kinase; D047908:Intracellular Signaling Peptides and Proteins; D008869:Microtubule-Associated Proteins; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D033902:Periplasmic Binding Proteins; D010766:Phosphorylation; D010770:Phosphotransferases / Q000235:genetics*; D010940:Plant Proteins / Q000378:metabolism*; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D011506:Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics; D015398:Signal Transduction / Q000502:physiology; D014157:Transcription Factors / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases; D001616:beta-Galactosidase / Q000378:metabolism; D056427:t-Complex Genome Region
C117966:AHP1 protein, Arabidopsis; C117967:AHP2 protein, Arabidopsis; C117968:AHP3 protein, Arabidopsis; C117138:ARR1 protein, Arabidopsis; D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; D033902:Periplasmic Binding Proteins; D010940:Plant Proteins; D011506:Proteins; C585559:TCP10 protein, Arabidopsis; C533392:TCP10L3 protein, human; D014157:Transcription Factors; C018963:histidine-binding protein; D001224:Aspartic Acid; D006639:Histidine; C116196:PPP1R11 protein, human; D044767:Ubiquitin-Protein Ligases; D010770:Phosphotransferases; D011494:Protein Kinases; D000071677:Histidine Kinase; D001616:beta-Galactosidase