title: Expression of tumor necrosis factor alpha and interleukin 1 beta in jejuna of volunteers after experimental challenge with Cryptosporidium parvum correlates with exposure but not with symptoms.
abstract: Jejunal biopsies from volunteers challenged with Cryptosporidium parvum were examined for tumor necrosis factor alpha (TNF-alpha) and interleukin (IL)-1 beta mRNA. Postchallenge biopsies from 15 of 28 (54%) volunteers expressed TNF-alpha; 14% expressed IL-1 beta. Cytokine expression did not correlate with enteric symptoms, suggesting that TNF-alpha and IL-1 beta are not key mediators of diarrhea in human cryptosporidiosis.
authors.affiliation: Department of Medicine, Baylor College of Medicine, Houston, Texas 77030, USA.
authors.forename: P
authors.initials: P
authors.lastname: Robinson
authors.forename: P C
authors.initials: PC
authors.lastname: Okhuysen
authors.forename: C L
authors.initials: CL
authors.lastname: Chappell
authors.forename: D E
authors.initials: DE
authors.lastname: Lewis
authors.forename: I
authors.initials: I
authors.lastname: Shahab
authors.forename: A
authors.initials: A
authors.lastname: Janecki
authors.forename: A C
authors.initials: AC
authors.lastname: White
mesh_terms: D000818:Animals; D001706:Biopsy; D003457:Cryptosporidiosis / Q000276:immunology*; D016785:Cryptosporidium parvum; D003967:Diarrhea / Q000209:etiology*; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000235:genetics; D019409:Interleukin-15 / Q000096:biosynthesis; D007583:Jejunum / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D007375:Interleukin-1; D019409:Interleukin-15; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha