title: T cell phenotypes of the normal nasal mucosa: induction of Th2 cytokines and CCR3 expression by IL-4.
abstract: Mucosal environments such as that of the nose are points of first contact between the human organism and its environment. At these sites the immune system must be regulated to differentiate between and respond appropriately to pathogens and harmless contaminants. T cell-driven immune responses broadly fall into Th1- or Th2-type phenotypes, with increasing evidence that the recruitment of these T lymphocyte subsets is mediated by selective expression of specific chemokine receptors. We have investigated the immunology of the normal nasal mucosa. We show that nasal T cell lines from normal individuals, expanded by culture in IL-2, show reduced expression of the Th2-type cytokines IL-4 and IL-5 compared with lines derived from the blood of the same subjects. These T cells also show reduced expression of the Th2-selective chemokine receptor, CCR3, but similar levels of CCR4 compared with the blood-derived lines. This apparent suppression of Th2 cytokine and CCR3 expression by nasal T cells was reversed by addition of IL-4 to the culture medium. These data are consistent with the presence of a nasal mucosal microenvironment that suppresses Th2 responses and may represent a protective measure against atopic allergic disease in humans and a favoring of Th1 responses to infectious agents. In contrast, T cell expression of CCR1 was higher in the nose than in the blood regardless of the culture medium cytokine environment in keeping with a role for this receptor in tissue homing or lymphocyte activation.
authors.affiliation: Upper Respiratory Medicine, National Heart and Lung Institute Division, and Leukocyte Biology Section, Biomedical Sciences Division, Imperial College School of Medicine, London, United Kingdom.
authors.forename: S
authors.initials: S
authors.lastname: Till
authors.forename: L
authors.initials: L
authors.lastname: Jopling
authors.forename: P
authors.initials: P
authors.lastname: Wachholz
authors.forename: R
authors.initials: R
authors.lastname: Robson
authors.forename: S
authors.initials: S
authors.lastname: Qin
authors.forename: D
authors.initials: D
authors.lastname: Andrew
authors.forename: L
authors.initials: L
authors.lastname: Wu
authors.forename: J
authors.initials: J
authors.lastname: van Neerven
authors.forename: T
authors.initials: T
authors.lastname: Williams
authors.forename: S
authors.initials: S
authors.lastname: Durham
authors.forename: I
authors.initials: I
authors.lastname: Sabroe
mesh_terms: D000328:Adult; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis* / Q000097:blood; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D015847:Interleukin-4 / Q000494:pharmacology*; D008213:Lymphocyte Activation; D008297:Male; D009297:Nasal Mucosa / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D054397:Receptors, CCR3; D019707:Receptors, Chemokine / Q000096:biosynthesis* / Q000097:blood; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D018418:Th2 Cells / Q000276:immunology* / Q000378:metabolism
chemical_list: C515350:CCR3 protein, human; D016207:Cytokines; D054397:Receptors, CCR3; D019707:Receptors, Chemokine; D015847:Interleukin-4