title: Denaturation and partial renaturation of a tightly tetramerized DsRed protein under mildly acidic conditions.
abstract: The red fluorescent protein, DsRed, recently cloned from coral Discosoma sp. has one of the longest fluorescence waves and one of the most complex absorbance spectra among the family of fluorescent proteins. In this work we found that with time DsRed fluorescence decreases under mildly acidic conditions (pH 4.0-4.8) in a pH-dependent manner, and this fluorescence inactivation could be partially recovered by subsequent re-alkalization. The DsRed absorbance and circular dichroism spectra under these conditions revealed that the fluorescence changes were caused by denaturation followed by partial renaturation of the protein. Further, analytical ultracentrifugation determined that native DsRed formed a tight tetramer under various native conditions. Quantitative analysis of the data showed that several distinct states of protein exist during the fluorescence inactivation and recovery, and the inactivation of fluorescence can be caused by protonation of a single ionogenic group in each monomer of DsRed tetramer.
authors.affiliation: Center for Molecular Medicine, Moscow State University, Moscow, Russia. vrzh@genebee.msu.ru
authors.forename: P V
authors.initials: PV
authors.lastname: Vrzheshch
authors.forename: N A
authors.initials: NA
authors.lastname: Akovbian
authors.forename: S D
authors.initials: SD
authors.lastname: Varfolomeyev
authors.forename: V V
authors.initials: VV
authors.lastname: Verkhusha
mesh_terms: D000818:Animals; D002942:Circular Dichroism; D003063:Cnidaria; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008164:Luminescent Proteins / Q000737:chemistry* / Q000378:metabolism*; D011487:Protein Conformation; D011489:Protein Denaturation; D020673:Protein Renaturation; D013050:Spectrometry, Fluorescence
chemical_list: D008164:Luminescent Proteins; C413663:fluorescent protein 583