title: Production and characterization of a thermostable alpha-amylase from Nocardiopsis sp. endophyte of yam bean.
abstract: Thermostable amylolytic enzymes have been currently investigated to improve industrial processes of starch degradation. Studies on production of alpha-amylase by Nocardiopsis sp., an endophytic actinomycete isolated from yam bean (Pachyrhizus erosus L. Urban), showed that higher enzyme levels were obtained at the end of the logarithmic growth phase after incubation for 72 h at pH 8.6. Maximum activity of alpha-amylase was obtained at pH 5.0 and 70 degrees C. The isolated enzyme exhibited thermostable properties as indicated by retention of 100% of residual activity at 70 degrees C, and 50% of residual activity at 90 degrees C for 10 min. Extracellular enzyme from Nocardiopsis sp. was purified by fractional precipitation with ammonium sulphate. After 60% saturation produced 1130 U mg-1 protein and yield was 28% with purification 2.7-fold. The enzyme produced by Nocardiopsis sp. has potential for industrial applications.
authors.affiliation: Department of Nutrition, Department of Biochemistry, Department of Antibiotics, University of Federal Pernambuco, 50670-901 Recife, Brazil. newtonps@novaera.com.br
authors.forename: T L
authors.initials: TL
authors.lastname: Stamford
authors.forename: N P
authors.initials: NP
authors.lastname: Stamford
authors.forename: L C
authors.initials: LC
authors.lastname: Coelho
authors.forename: J M
authors.initials: JM
authors.lastname: Araújo
mesh_terms: D000192:Actinomycetales / Q000201:enzymology* / Q000302:isolation & purification; D001673:Biodegradation, Environmental; D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology; D004795:Enzyme Stability; D007887:Fabaceae / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010946:Plants, Medicinal; D013213:Starch / Q000378:metabolism; D013696:Temperature; D000516:alpha-Amylases / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D013213:Starch; D000516:alpha-Amylases