Determination of organochlorine and organophosphorus pesticide residues in eggs using a solid phase extraction cleanup.
A multiresidue solid phase extraction (SPE) method for the isolation and subsequent gas chromatographic determination of nonpolar organochlorine and polar organophosphorus pesticide residues in eggs is described. The method uses an acetonitrile extraction followed by an SPE cleanup using graphitized carbon black and aminopropyl SPE columns. Organophosphorus pesticides are determined by gas chromatography with flame photometric detection. After further cleanup of the extract using Florisil SPE columns, organochlorine pesticides are determined by gas chromatography with electron capture detection. Studies were performed using eggs containing both fortified and incurred pesticide residues. The average recoveries were 86-108% for 8 fortified organochlorine pesticide residues and 61-149% for 28 fortified organophosphorus pesticide residues.
Schenck
F J
FJ
Center for Veterinary Medicine, U.S. Food and Drug Administration, 8401 Muirkirk Road, Laurel, Maryland 20708, USA. fschenck@ora.fda.gov
Donoghue
D J
DJ
D000818:Animals; D002645:Chickens; D002849:Chromatography, Gas / Q000379:methods; D004531:Eggs / Q000032:analysis*; D005260:Female; D007306:Insecticides / Q000032:analysis / Q000302:isolation & purification*; D009943:Organophosphorus Compounds / Q000032:analysis / Q000302:isolation & purification*; D010573:Pesticide Residues / Q000032:analysis / Q000302:isolation & purification*
D007306:Insecticides; D009943:Organophosphorus Compounds; D010573:Pesticide Residues