A clinical trial on a plate? The potential of 384-well format solid phase extraction for high-throughput bioanalysis using liquid chromatography/tandem mass spectrometry.
The application of 384-well format solid phase extraction (SPE) for bioanalysis using liquid chromatography/tandem mass spectrometry (LC/MS/MS) is reported and a 384-well SPE method for the 5-HT agonist sumatriptan in human plasma described. Plasma samples were extracted on a prototype low-density polyethylene 384-well SPE block using a packed bed of 5 mg Oasistrade mark HLB. Liquid handling was automated by a combination of a robotic sampler processor and a 96/384 multi-channel dispensing station. Samples and SPE reagents were drawn through the SPE block by centrifugation. The extracts were analysed by LC/MS/MS with thermally and pneumatically assisted electrospray ionisation and selected reaction monitoring. The method is used to illustrate and discuss the feasibility and viability of sample preparation techniques in high-density microtitre plate format for routine bioanalysis.
Biddlecombe
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RA
Department of International Bioanalysis, Division of Bioanalysis and Drug Metabolism, Glaxo Wellcome R&D, Park Road, Ware, Hertfordshire SG12 0DP, UK.
Benevides
C
C
Pleasance
S
S
D001774:Blood Chemical Analysis / Q000295:instrumentation* / Q000592:standards; D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000592:standards; D002986:Clinical Trials as Topic / Q000295:instrumentation; D006801:Humans; D013058:Mass Spectrometry / Q000295:instrumentation* / Q000592:standards; D017366:Serotonin Receptor Agonists / Q000097:blood; D018170:Sumatriptan / Q000097:blood
D017366:Serotonin Receptor Agonists; D018170:Sumatriptan