title: Purification and crystallization of Japanese pear S-RNase associated with gametophytic self-incompatibility.
abstract: S-RNase is a 25 kDa pistil-specific protein associated with gametophytic self-incompatibility. The S-RNase is secreted into the transmitting tissue of the pistil and is responsible for the discrimination of pollen S-alleles. Crystals of Japanese pear S(3)-RNase were obtained by the hanging-drop vapour-diffusion method. Preliminary X-ray data showed that the crystals diffract to a 1.5 A resolution and belong to the space group P2(1), with unit-cell parameters a = 45.4, b = 52.4, c = 47.4 A, alpha = gamma = 90, beta = 106.5 degrees.
authors.affiliation: Division of Protein Chemistry, Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Matsuura
authors.forename: M
authors.initials: M
authors.lastname: Unno
authors.forename: H
authors.initials: H
authors.lastname: Sakai
authors.forename: T
authors.initials: T
authors.lastname: Tsukihara
authors.forename: S
authors.initials: S
authors.lastname: Norioka
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D005638:Fruit / Q000201:enzymology* / Q000502:physiology; D011487:Protein Conformation; D012260:Ribonucleases / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D012260:Ribonucleases