Rapid identification of virulent type I strains of the protozoan pathogen Toxoplasma gondii by PCR-restriction fragment length polymorphism analysis at the B1 gene.
Sequence analysis at the 35-fold-repetitive B1 locus identified three restriction sites capable of discriminating type I (mouse-virulent) from type II or III (mouse-avirulent) strains of Toxoplasma gondii. B1 PCR-restriction fragment length polymorphism analysis of 8 type I, 17 type II, and 8 type III strains confirms the specificity of the assay. It should now be possible to ask whether strain genotype affects the severity and type of clinical disease in humans.
Grigg
M E
ME
Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California 94305-5124, USA.
Boothroyd
J C
JC
D000818:Animals; D016054:DNA, Protozoan / Q000235:genetics; D017125:Genes, Protozoan; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D015800:Protozoan Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014122:Toxoplasma / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity*; D014123:Toxoplasmosis / Q000469:parasitology*; D014774:Virulence / Q000235:genetics
D016054:DNA, Protozoan; D015800:Protozoan Proteins