Mechanisms mediating Porphyromonas gingivalis gingipain RgpA-induced oral mucosa inflammation in vivo.
Suffusion of gingipain RgpA (GRgpA) elicited a significant concentration-dependent increase in the clearance of macromolecules from in situ hamster cheek pouch which was attenuated by NPC 17647, a selective bradykinin B(2) receptor antagonist. Leupeptin and a mixture of proteinase inhibitors also attenuated GRgpA-induced responses. These data indicate that GRgpA elicits plasma exudation from in situ oral mucosa in a catalytic site-dependent fashion by elaborating bradykinin.
Rubinstein
I
I
Department of Medicine, University of Illinois at Chicago and VA Chicago Health Care System West Side Division, Chicago, Illinois 60612, USA. IRubinst@uic.edu
Potempa
J
J
Travis
J
J
Gao
X P
XP
D018829:Adhesins, Bacterial / Q000633:toxicity*; D000818:Animals; D001920:Bradykinin / Q000502:physiology; D002199:Capillary Permeability / Q000187:drug effects; D020134:Catalytic Domain; D006224:Cricetinae; D003546:Cysteine Endopeptidases / Q000633:toxicity*; D000080867:Gingipain Cysteine Endopeptidases; D006388:Hemagglutinins / Q000633:toxicity*; D007213:Indomethacin / Q000494:pharmacology; D007976:Leupeptins / Q000494:pharmacology; D008297:Male; D008647:Mesocricetus; D009061:Mouth Mucosa / Q000187:drug effects*; D010518:Periodontitis / Q000188:drug therapy / Q000209:etiology*; D043782:Receptor, Bradykinin B2; D018002:Receptors, Bradykinin / Q000502:physiology
D018829:Adhesins, Bacterial; D000080867:Gingipain Cysteine Endopeptidases; D006388:Hemagglutinins; D007976:Leupeptins; D043782:Receptor, Bradykinin B2; D018002:Receptors, Bradykinin; D003546:Cysteine Endopeptidases; C032854:leupeptin; D001920:Bradykinin; D007213:Indomethacin