title: Association of Trk neurotrophin receptors with components of the cytoplasmic dynein motor.
abstract: Nerve growth factor (NGF) initiates its trophic effects by long-range signaling through binding, internalization, and transport of a ligand-receptor complex from the axon terminal to the cell body. However, the mechanism by which retrograde transport of NGF takes place has not been elucidated. Here we describe an interaction between the Trk receptor tyrosine kinase and a 14 kDa light chain of cytoplasmic dynein. After transfection in human embryonic kidney 293 cells, this 14 kDa dynein light chain was found to bind to TrkA, TrkB, and TrkC receptors. Mapping experiments indicated that the 14 kDa dynein light chain binds to the distal region of the TrkA juxtamembrane domain. Coimmunoprecipitation experiments in vivo indicate that Trk receptors are in a complex with the 14 kDa light chain and 74 kDa intermediate chain of dynein. Confirming the physiological relevance of this association, a marked accumulation of Trk with the 14 kDa and the 74 kDa dynein components was observed after ligation of the sciatic nerve. The association of Trk receptors with components of cytoplasmic dynein suggests that transport of neurotrophins during vesicular trafficking may occur through a direct interaction of the Trk receptor with the dynein motor machinery.
authors.affiliation: Molecular Neurobiology Program, Skirball Institute for Biomolecular Medicine, Departments of Cell Biology , New York, New York 10016, USA.
authors.forename: H
authors.initials: H
authors.lastname: Yano
authors.forename: F S
authors.initials: FS
authors.lastname: Lee
authors.forename: H
authors.initials: H
authors.lastname: Kong
authors.forename: J
authors.initials: J
authors.lastname: Chuang
authors.forename: J
authors.initials: J
authors.lastname: Arevalo
authors.forename: P
authors.initials: P
authors.lastname: Perez
authors.forename: C
authors.initials: C
authors.lastname: Sung
authors.forename: M V
authors.initials: MV
authors.lastname: Chao
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism*; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000378:metabolism; D008026:Ligation; D008868:Microtubule Proteins / Q000235:genetics / Q000378:metabolism*; D008869:Microtubule-Associated Proteins; D020409:Molecular Motor Proteins / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D009687:Nuclear Proteins; D011233:Precipitin Tests; D011485:Protein Binding / Q000502:physiology; D051381:Rats; D020917:Receptor, trkA / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012584:Sciatic Nerve / Q000502:physiology; D017384:Sequence Deletion; D014162:Transfection; D020798:Two-Hybrid System Techniques; D056427:t-Complex Genome Region
chemical_list: D008868:Microtubule Proteins; D008869:Microtubule-Associated Proteins; D020409:Molecular Motor Proteins; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; D020917:Receptor, trkA