Neurotrophic effects of FPF-1070 (Cerebrolysin) on cultured neurons from chicken embryo dorsal root ganglia, ciliary ganglia, and sympathetic trunks.
We examined the effect of FPF-1070 (Cerebrolysin) on neurite outgrowth in explant cultures of dorsal root ganglia (DRG), sympathetic trunks (ST), and ciliary ganglia (CG) from 10- to 11-day chicken embryos. FPF-1070 significantly promoted neurite outgrowth in DRG and ST neurons at all concentrations examined, in comparison with phosphate buffered saline-treated negative controls; however, this effect on neurite outgrowth was not as significant as that observed for nerve growth factor-treated positive controls on DRG and ST neurons. Additionally, FPF-1070 exhibited an inverted U relationship between concentration and effectiveness in DRG and ST neurons. In contrast, FPF-1070 did not affect neurite outgrowth in CG neurons although ciliary neurotrophic factor-treated positive controls showed striking neurite outgrowth. Our results demonstrate that FPF-1070 has different neurotrophic effects depending on the subpopulation of neurons. This study clarifies a role for neurotrophic activity in the mechanism of action of FPF-1070.
Satou
T
T
2nd Department of Pathology, Kinki University School of Medicine, Osakasayama, Osaka, Japan.
Itoh
T
T
Tamai
Y
Y
Ohde
H
H
Anderson
A J
AJ
Hashimoto
S
S
D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D020934:Ciliary Neurotrophic Factor / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005726:Ganglia, Parasympathetic / Q000166:cytology / Q000187:drug effects*; D005727:Ganglia, Spinal / Q000166:cytology / Q000187:drug effects*; D009412:Nerve Fibers / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D020932:Nerve Growth Factor / Q000494:pharmacology; D016501:Neurites / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D009474:Neurons / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D009994:Osmolar Concentration; D013564:Sympathetic Nervous System / Q000166:cytology / Q000187:drug effects*
D000596:Amino Acids; D020934:Ciliary Neurotrophic Factor; C006952:cerebrolysin; D020932:Nerve Growth Factor