Antihyperglycemic effect of T-1095 via inhibition of renal Na+-glucose cotransporters in streptozotocin-induced diabetic rats.
T-1095, a derivative of phlorizin, is an orally active inhibitor of Na+-glucose cotransporter (SGLT). We investigated the acute antihyperglycemic effect of T-1095 in streptozotocin-induced diabetic rats (STZ rats). T-1095 and its metabolite T-1095A inhibited the SGLT activity in brush border membranes prepared from kidneys of both normal and STZ rats, but the latter agent was approximately 10 times more potent than the former. Single oral administration of T-1095 (30-100 mg/kg) dose-dependently induced glycosuria in normal rats. The fed glucose levels in STZ rats were dose-dependently suppressed by single oral administration of T-1095 (3-100 mg/kg), whereas there was only marginal hypoglycemic effect in normal rats. Since there was no effect on blood glucose in nephrectomized STZ rats, inhibition of renal glucose reabsorption rather than intestinal glucose absorption mainly contributes to the antihyperglycemic effect of T-1095. In conclusion, T-1095 is the first orally active agent which has an acute antihyperglycemic action in the absence of endogenous insulin secretion with a low risk of hypoglycemia and has therapeutic potential for treatment of diabetes mellitus.
Oku
A
A
Discovery Research Laboratory, Tanabe Seiyaku Co. Ltd., Toda, Saitama, Japan.
Ueta
K
K
Arakawa
K
K
Kano-Ishihara
T
T
Matsumoto
M
M
Adachi
T
T
Yasuda
K
K
Tsuda
K
K
Saito
A
A
D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D002254:Carbonates / Q000494:pharmacology*; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy* / Q000652:urine; D005960:Glucosides / Q000494:pharmacology*; D006029:Glycosuria / Q000652:urine; D006943:Hyperglycemia / Q000097:blood / Q000188:drug therapy / Q000652:urine; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D008871:Microvilli / Q000187:drug effects / Q000378:metabolism; D009004:Monosaccharide Transport Proteins / Q000037:antagonists & inhibitors*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar
D001786:Blood Glucose; D002254:Carbonates; D005960:Glucosides; D007004:Hypoglycemic Agents; D009004:Monosaccharide Transport Proteins; C121396:T 1095