Circulating nucleic acids of Chlamydia pneumoniae and cytomegalovirus in patients undergoing coronary angiography.
Peripheral blood mononuclear cells from 208 consecutive patients undergoing elective coronary angiography or angioplasty were collected before, immediately after, and 4 h after the procedure. Nucleic acids of Chlamydia pneumoniae and of cytomegalovirus (CMV) were detected by PCR and confirmed by hybridization. Circulating C. pneumoniae DNA was identified in 24 patients (11.5%) and was associated with current smoking (odds ratio [OR] = 4.5, 95% confidence interval [CI] = 1.6 to 12.2, P = 0.004) but not with arterial narrowing on coronary angiogram or with serological results positive for C. pneumoniae. Circulating CMV DNA was identified in 36 patients (17.3%) and was associated with anti-CMV immunoglobulin G (OR = 2.7, 95% CI = 1.2 to 6.3, P = 0.02) but not with angiographic arterial narrowing or with the need for revascularization. Neither C. pneumoniae nor CMV DNA detection increased after angioplasty, a procedure in which endothelium is disrupted. Larger prospective studies are needed to determine the prognostic significance of DNA detection.
Smieja
M
M
Hamilton Regional Laboratory Medicine Programme, McMaster University, Hamilton, Ontario, Canada. smiejam@mcmaster.ca
Chong
S
S
Natarajan
M
M
Petrich
A
A
Rainen
L
L
Mahony
J B
JB
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015906:Angioplasty, Balloon, Coronary; D000907:Antibodies, Bacterial / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D016993:Chlamydophila pneumoniae / Q000302:isolation & purification*; D017023:Coronary Angiography; D003327:Coronary Disease / Q000000981:diagnostic imaging / Q000382:microbiology* / Q000628:therapy; D003587:Cytomegalovirus / Q000276:immunology / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000097:blood*; D004279:DNA, Viral / Q000097:blood*; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D016133:Polymerase Chain Reaction / Q000379:methods; D011379:Prognosis; D012044:Regression Analysis; D012907:Smoking
D000907:Antibodies, Bacterial; D000914:Antibodies, Viral; D004269:DNA, Bacterial; D004279:DNA, Viral; D007070:Immunoglobulin A; D007074:Immunoglobulin G