GABAergic modulation of neurons in the nucleus of the solitary tract with ascending projections to the subfornical organ in the rat.
Twenty-five neurons in the region of the nucleus of the solitary tract (NTS) were antidromically activated by electrical stimulation of the subfornical organ (SFO) in male rats under urethane anesthesia. Microiontophoretically applied bicuculline, a gamma-aminobutyric acid (GABA)(A) antagonist, but not phaclofen, a GABA(B) antagonist, attenuated the post-antidromic inhibitory response evoked by SFO stimulation of approximately two-third (n=17) of identified neurons, indicating the existence of recurrent inhibitory systems through GABA(A) receptors. Iontophoretically applied GABA decreased the spontaneous activity of all identified neurons, and the GABA-induced inhibition was prevented by simultaneously applied bicuculline, but not by phaclofen. Activation of peripheral baroreceptors, achieved by rising arterial blood pressure with an intravenous infusions of phenylepherine, suppressed the activity of the majority (n=20) of identified neurons. The inhibitory response of identified neurons (n=7) to baroreceptor activation was partially antagonized by iontophoretically applied bicuculline, but not by phaclofen. These results imply that GABAergic mechanisms may modulate the baroreceptor reflex acting on GABA(A) receptors of NTS neurons with ascending projections to the SFO in the region of the NTS.
Tanaka
J
J
Department of Human Development, Naruto University of Education, Naruto, 772-8502, Tokushima, Japan.
Miyakubo
H
H
Nomura
S
S
Sakamaki
K
K
Okumura
T
T
Hayashi
Y
Y
D000818:Animals; D001418:Baclofen / Q000031:analogs & derivatives* / Q000494:pharmacology; D001640:Bicuculline / Q000494:pharmacology; D004558:Electric Stimulation; D004594:Electrophysiology; D018756:GABA Antagonists / Q000494:pharmacology; D007478:Iontophoresis; D008297:Male; D009434:Neural Pathways; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D011311:Pressoreceptors / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D017552:Solitary Nucleus / Q000166:cytology* / Q000502:physiology; D013356:Subfornical Organ / Q000166:cytology* / Q000502:physiology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D018756:GABA Antagonists; C052329:phaclofen; D005680:gamma-Aminobutyric Acid; D001418:Baclofen; D001640:Bicuculline