title: Immunocytochemical study of cell proliferation in the cystic ovarian follicles in cows.
abstract: We examined the frequency of proliferating cells in cystic, atretic and healthy antral follicles to determine whether a disorder of cell proliferation was responsible for the occurrence of bovine cystic follicles. Paraffin sections of healthy follicles and various stages of atretic and cystic follicles were immunostained with mouse monoclonal antibody to proliferating cell nuclear antigen (PCNA). The PCNA-positive cells were counted in 4 different regions of a follicle from the apical to the basal side. In the granulosa layer, a significantly higher frequency of PCNA-positive cells was observed in the healthy follicle in the basal region as compared with the apical region. A similar pattern of PCNA-positive cells population was observed in the granulosa layer of atretic follicles, although the frequency in the basal region was significantly lower in the atretic than the healthy follicle. The rate of cell proliferation in the granulosa layer of cystic follicles was markedly lower at the basal region than that of atretic follicles. In the theca interna, the frequency of PCNA-positive cells in atretic follicles at the early stages was higher than that in cystic follicles at the early stages. These results suggest that in the healthy follicle the proliferative activity of granulosa cells is higher in the basal than the apical region, and that the cell proliferation activity in the granulosa and theca interna may decrease in association with the induction of a follicular cyst.
authors.affiliation: Graduate School for International Development and Cooperation, Hiroshima University, Higashi-Hiroshima, Japan. niso@hiroshima-u.ac.jp
authors.forename: N
authors.initials: N
authors.lastname: Isobe
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshimura
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D002418:Cattle Diseases / Q000473:pathology*; D002455:Cell Division; D005260:Female; D005496:Follicular Atresia; D006107:Granulosa Cells / Q000473:pathology; D007150:Immunohistochemistry; D010048:Ovarian Cysts / Q000473:pathology / Q000662:veterinary*; D006080:Ovarian Follicle / Q000737:chemistry / Q000473:pathology*; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D013799:Theca Cells / Q000473:pathology
chemical_list: D000911:Antibodies, Monoclonal; D018809:Proliferating Cell Nuclear Antigen