Determination of specific radioactivity for (76)Br-labeled compounds measuring the ratio between (76)Br and (79)Br using packed capillary liquid chromatography mass spectrometry.
Packed capillary liquid chromatography with electrospray mass spectrometry was used for direct determination of the specific radioactivity by calculation of isotope ratios between the (76)Br- and (79)Br-labeled analogues of N-((3-aminomethyl)benzyl)-4-bromobenzamide. Using 20 microL injections on packed capillary columns, sufficient mass sensitivity was attained for the determination on an injected amount of radioactivity corresponding to approximately 2 MBq (0.3 pmol of the (76)Br isotopic analogue).
Forngren
B H
BH
Department of Analytical Chemistry, Institute of Chemistry, Uppsala University, Uppsala, Sweden.
Yngve
U
U
Forngren
T
T
Långström
B
B
D001549:Benzamides / Q000737:chemistry; D001966:Bromine / Q000032:analysis*; D001967:Bromine Radioisotopes; D002853:Chromatography, Liquid; D007202:Indicators and Reagents; D013058:Mass Spectrometry
D001549:Benzamides; D001967:Bromine Radioisotopes; D007202:Indicators and Reagents; C422196:N-((3-aminomethyl)benzyl)-4-bromobenzamide; D001966:Bromine