Kaposi's sarcoma-associated herpesvirus latent and lytic gene expression as revealed by DNA arrays.
Kaposi's sarcoma-associated herpesvirus (KSHV; human herpesvirus 8) is associated with three human tumors, Kaposi's sarcoma, primary effusion lymphoma (PEL), and multicentric Castleman's disease. KSHV encodes a number of homologs of cellular proteins involved in the cell cycle, signal transduction, and modulation of the host immune response. Of the virus complement of over 85 open reading frames (ORFs), the expression of only a minority has been characterized individually. We have constructed a nylon membrane-based DNA array which allows the expression of almost every ORF of KSHV to be measured simultaneously. A PEL-derived cell line, BC-3, was used to study the expression of KSHV during latency and after the induction of lytic replication. Cluster analysis, which arranges genes according to their expression profile, revealed a correlation between expression and assigned gene function that is consistent with the known stages of the herpesvirus life cycle. Furthermore, latent and lytic genes thought to be functionally related cluster into groups. The correlation between gene expression and function also infers possible roles for KSHV genes yet to be characterized.
Jenner
R G
RG
Wohl Virion Centre, Department of Immunology and Molecular Pathology, Windeyer Institute, University College London, London W1T 4JF, United Kingdom.
Albà
M M
MM
Boshoff
C
C
Kellam
P
P
D000595:Amino Acid Sequence; D002460:Cell Line; D016000:Cluster Analysis; D004268:DNA-Binding Proteins / Q000235:genetics; D015870:Gene Expression; D020869:Gene Expression Profiling; D019288:Herpesvirus 8, Human / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D006801:Humans; D050835:Interferon Regulatory Factors; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016366:Open Reading Frames / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D014764:Viral Proteins; D017735:Virus Latency; D014779:Virus Replication / Q000235:genetics*
D004268:DNA-Binding Proteins; D050835:Interferon Regulatory Factors; D014157:Transcription Factors; D014764:Viral Proteins; C413486:viral interferon regulatory factors