title: [Detection of genetically modified soy (Roundup-Ready) in processed food products].
abstract: In this study, the application of a qualitative and a quantitative method of analysis to detect genetically modified RR-Soy (Roundup-Ready Soy) in processed foods is described. A total of 179 various products containing soy such as baby food and diet products, soy drinks and desserts, tofu and tofu products, soy based meat substitutes, soy protein, breads, flour, granules, cereals, noodles, soy bean sprouts, fats and oils as well as condiments were investigated following the pattern of the section 35 LMBG-method L 23.01.22-1. The DNA was extracted from the samples and analysed using a soybean specific lectin gene PCR as well as a PCR, specific for the genetic modification. Additional, by means of PCR in combination with fluorescence-detection (TaqMan 5'-Nuclease Assay), suspicious samples were subjected to a real-time quantification of the percentage of genetically modified RR-Soy. The methods of analysis proved to be extremely sensitive and specific in regard to the food groups checked. The fats and oils, as well as the condiments were the exceptions in which amplifiable soy DNA could not be detected. The genetic modification of RR-Soy was detected in 34 samples. Eight of these samples contained more than 1% of RR-Soy. It is necessary to determine the percentage of transgenic soy in order to assess whether genetically modified ingredients were deliberately added, or whether they were caused by technically unavoidable contamination (for example during transportation and processing).
authors.affiliation: Staatliches Veterinäruntersuchungsamt Detmold.
authors.forename: M
authors.initials: M
authors.lastname: Hagen
authors.forename: B
authors.initials: B
authors.lastname: Beneke
mesh_terms: D001628:Beverages / Q000032:analysis; D005504:Food Analysis; D005511:Food Handling; D006801:Humans; D007223:Infant; D007225:Infant Food / Q000032:analysis; D037102:Lectins / Q000032:analysis / Q000235:genetics; D037121:Plant Lectins; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D030262:Soybean Proteins; D013025:Glycine max / Q000235:genetics*; D010835:Phytohemagglutinins
chemical_list: D037102:Lectins; D037121:Plant Lectins; D030262:Soybean Proteins; C028824:soybean lectin; D010835:Phytohemagglutinins