title: Elevated interstitial adenosine concentrations do not activate the muscle reflex.
abstract: The purpose of the present study was to examine the effects of adenosine perfusion of the isolated triceps surae muscle group in the decerebrate cat on interstitial adenosine concentrations as well as heart rate and blood pressure responses. In six male cats (6.0 +/- 0.21 kg), the triceps surae muscle group of both legs was perfused with an artificial blood solution containing no additives (control) and then with blood containing 20 mM or 100 microM adenosine for 10 min. An intact muscle reflex was confirmed by bolus injections of 50 mM phosphate and/or saturated KCl administered into the triceps surae muscle via the cannulated popliteal artery before and after adenosine blood perfusion. Microdialysis of the triceps surae muscle group during muscle perfusion revealed that interstitial adenosine was elevated (P < 0.05) from 0.9 +/- 0.3 microM during control blood perfusion to 2,421 +/- 547 microM during 20 mM adenosine perfusion. In addition, interstitial adenosine levels were increased (P < 0.05) from 1.1 +/- 0.3 microM during control blood perfusion to 4.1 +/- 1.2 microM during perfusion with 100 microM adenosine. Despite the large increases in interstitial adenosine levels, perfusion of the triceps surae muscle group with the two blood adenosine solutions resulted in no significant increases in heart rate or blood pressure. These data strongly suggest that elevated interstitial adenosine concentrations do not play a role in activating the muscle reflex and confirm our previous in vivo human findings (J Appl Physiol 83: 1045-1053, 1997).
authors.affiliation: Division of Cardiology, Department of Medicine, The Pennsylvania State University College of Medicine, The Milton S. Hershey Medical Center, Hershey Pennsylvania, 17033, USA. dmaclean@psu.edu
authors.forename: D A
authors.initials: DA
authors.lastname: MacLean
authors.forename: L M
authors.initials: LM
authors.lastname: Vickery
authors.forename: L I
authors.initials: LI
authors.lastname: Sinoway
mesh_terms: D000241:Adenosine / Q000494:pharmacology*; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D002415:Cats; D002628:Chemoreceptor Cells / Q000187:drug effects / Q000502:physiology; D005110:Extracellular Space; D006339:Heart Rate / Q000187:drug effects; D008297:Male; D017551:Microdialysis; D018482:Muscle, Skeletal / Q000502:physiology*; D009475:Neurons, Afferent / Q000502:physiology; D010710:Phosphates / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D012018:Reflex / Q000187:drug effects* / Q000502:physiology*
chemical_list: D010710:Phosphates; D011189:Potassium Chloride; D000241:Adenosine; C018279:sodium phosphate