title: [Genotyping cytochrome P450IIE1 in alcoholic liver diseases and its significance].
abstract: OBJECTIVE
  To study the relationship between genotypes of cytochrome P450IIE1 (IIE1 ) and development of chronic liver diseases.
  
  
  METHODS
  Cytochrome P450IIE1 was genotyped by PCR and restricted endonucleases digestion of DNA prepared from peripheral white blood cells.
  
  
  RESULTS
  IIE1 could be divided into three types: A, B and C based on different products digested by two endonucleases. In normal controls predominant genotypes were A and B. No relationship was found between development of non-alcoholic chronic liver diseases and IIE1 genotypes. Its genotype distribution was similar to normal controls. Detection of type A in alcoholic liver diseases was significantly decreased and type B was significantly increased (P<0.01). Detection of type A in alcohol-related hepatocellular carcinoma was also decreased and type B increased compared to non-alcohol-related hepatocellular carcinoma.
  
  
  CONCLUSIONS
  IIE1 genotypes play some roles in development of alcoholic liver diseases and alcohol-related hepatocellular carcinoma.
authors.affiliation: Department of Gastroenterology, Zhongshan Hospital, Shanghai 200032, China.
authors.forename: S
authors.initials: S
authors.lastname: Zhang
authors.forename: S
authors.initials: S
authors.lastname: Liu
authors.forename: H
authors.initials: H
authors.lastname: Liu
authors.forename: W
authors.initials: W
authors.lastname: Zhu
mesh_terms: D000328:Adult; D006528:Carcinoma, Hepatocellular / Q000201:enzymology / Q000235:genetics; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008108:Liver Diseases, Alcoholic / Q000201:enzymology* / Q000235:genetics; D008113:Liver Neoplasms / Q000201:enzymology / Q000235:genetics; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic
chemical_list: D019392:Cytochrome P-450 CYP2E1