[Inhibitory effect of nicardipine on fibroblast proliferation mechanisms].
OBJECTIVE
To assess the effect of nicardipine (NCP) on fibroblast migration and proliferation, and its cellular toxicity.


METHODS
In vitro wound repair was assessed in confluent fibroblast monolayer. Mechanical round wounds were performed in the monolayers and the cultures were incubated in fresh media plus NCP. The cell-free area was monitored after 0, 18, 24 and 48 hours. Groups of treatment: Group 1, Sham. Group 2, NCP 10(-4)M in the media. Group 3, NCP 7.5x10(-5)M. Group 4, NCP 5x10(-5)M. Group 5, NCP 2.5x10(-5)M. Group 6, NCP 10(-5)M. Group 7, NCP 10(-6)M. Group 8, NCP 10(-7)M. Group 9, NCP 10(-3)M. Each experiment consisted of three tests that were repeated four times.


RESULTS
The fibroblast migration and proliferation was inhibited at 5x10(-5)M or higher doses. The proliferation after 48 hours with NCP 2.5x10(-5)M was statistically inferior to the control group and groups 7, 8, and 9. NCP 5x10(-5)M or higher doses showed cellular atypia and cell death.


CONCLUSIONS
NCP effectively inhibits fibroblastic wound repair process at doses 2.5x10(-5)M and shows toxicity at doses over 5x10(-5)M.
Pinilla
I
I
Servicio de Oftalmología, Hospital Universitario Miguel Servet, Zaragoza, Unidad Mixta de Investigación, Universidad de Zaragoza, España.
Izaguirre
L B
LB
Gonzalvo
F J
FJ
Jiménez
P
P
Piazuelo
E
E
Polo
V
V
Larrosa
J M
JM
Honrubia
F M
FM
D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects*; D006801:Humans; D009529:Nicardipine / Q000494:pharmacology*
D002121:Calcium Channel Blockers; D009529:Nicardipine