Characterization and purification of a bacteriocin produced by a potential probiotic culture, Lactobacillus acidophilus 30SC.
Lactobacillus acidophilus 30SC was tested for its potential as a probiotic culture. The strain exhibited good acid tolerance in an artificial gastric solution as well as bile resistance in media containing 0.3% bile acids. The strain produced a heat-stable antimicrobial compound that was shown to be proteinaceous in nature and, therefore, referred to as a bacteriocin. The bacteriocin was active over a wide pH range and inhibited a number of gram-positive bacteria including Listeria ivanovii and pathogenic strains. The bacteriocin was purified by 50% ammonium sulfate precipitation followed by hydrophobic interaction column chromatography. The SDS-PAGE of the active fractions resulted in a single band with estimated molecular mass of 3.5 kDa. These results demonstrate the potential of L. acidophilus 30SC as a probiotic culture that can be utilized in the manufacturing of dairy foods and dietary supplements.
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Department of Food Science & Technology, New York State Agricultural Experiment Station, Cornell University, Geneva 14456-0462, USA.
Kim
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Worobo
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D001430:Bacteriocins / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification; D001646:Bile / Q000737:chemistry; D002845:Chromatography; D015169:Colony Count, Microbial; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007779:Lactobacillus acidophilus / Q000378:metabolism*; D008970:Molecular Weight; D019936:Probiotics / Q000378:metabolism*; D013997:Time Factors
D001430:Bacteriocins