title: SigE is a chaperone for the Salmonella enterica serovar Typhimurium invasion protein SigD.
abstract: SigD is translocated into eucaryotic cells by a type III secretion system. In this work, evidence that the putative chaperone SigE directly interacts with SigD is presented. A bacterial two-hybrid system demonstrated that SigE can interact with itself and SigD. In addition, SigD was specifically copurified with SigE-His(6) on a nickel column.
authors.affiliation: Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
authors.forename: K H
authors.initials: KH
authors.lastname: Darwin
authors.forename: L S
authors.initials: LS
authors.lastname: Robinson
authors.forename: V L
authors.initials: VL
authors.lastname: Miller
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D005408:Flagellin / Q000378:metabolism*; D018832:Molecular Chaperones / Q000378:metabolism*; D011485:Protein Binding; D012486:Salmonella typhimurium / Q000378:metabolism* / Q000472:pathogenicity; D012808:Sigma Factor / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D001426:Bacterial Proteins; D018832:Molecular Chaperones; D012808:Sigma Factor; C111259:sigE protein, Bacteria; D005408:Flagellin; C416532:flaB flagellin