Effects of bilayer surface charge density on molecular adsorption and transport across liposome bilayers.
Second harmonic generation (SHG) was used to study both the adsorption of malachite green (MG), a positively charged organic dye, onto liposomes of different lipid compositions, and the transport kinetics of MG across the liposome bilayer in real time. We found that the dye adsorption increased linearly with the fraction of negatively charged lipids in the bilayer. Similarly, the transport rate constant for crossing the bilayer increased linearly with the fraction of charged lipid in the bilayer.
Liu
Y
Y
Department of Chemistry, Columbia University, New York, New York 10027, USA.
Yan
E C
EC
Eisenthal
K B
KB
D000327:Adsorption; D001692:Biological Transport; D055592:Biophysical Phenomena; D001703:Biophysics; D004578:Electron Spin Resonance Spectroscopy; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D012394:Rosaniline Dyes / Q000737:chemistry; D055672:Static Electricity; D013499:Surface Properties
D008051:Lipid Bilayers; D008081:Liposomes; D012394:Rosaniline Dyes; C005095:malachite green