Evaluation of a rapid optical immunoassay for influenza viruses (FLU OIA test) in comparison with cell culture and reverse transcription-PCR.
The FLU OIA test was evaluated with 146 throat swab specimens from subjects with a flu-like illness in six Canadian clinics during the 1999-2000 flu season. The rate of positivity of the FLU OIA test (41.5%) was significantly lower than that of cell culture (55.2%) or reverse transcription-PCR (55.9%) during a season in which only influenza A virus was detected.
Boivin
G
G
Research Center in Infectious Diseases of the Centre Hospitalier Universitaire de Québec and Laval University, Québec City, Quebec, Canada. Guy.Boivin@crchul.ulaval.ca
Hardy
I
I
Kress
A
A
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007223:Infant; D009980:Influenza A virus / Q000235:genetics / Q000302:isolation & purification*; D007252:Influenza Vaccines; D007251:Influenza, Human / Q000175:diagnosis* / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D010614:Pharynx / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods; D014773:Virology / Q000379:methods
D007252:Influenza Vaccines