The structure of pariacoto virus reveals a dodecahedral cage of duplex RNA.
The 3.0 A resolution crystal structure of Pariacoto virus (PaV) reveals extensive interactions between portions of the viral RNA genome and the icosahedral capsid. Under the protein shell of the T = 3 quasi equivalent capsid lies a dodecahedral cage composed of RNA duplex that accounts for approximately 35% of the single-stranded RNA genome. The highly basic N-terminal regions (residues 7-54) of the subunits, forming pentamers (A subunits) are clearly visible in the density map and make numerous interactions with the RNA cage. The C-terminal segments (residues 394-401) of the A subunits lie in channels near the quasi three-fold axes. Electron cryo-microscopy and image reconstruction of PaV particles clearly show the dodecahedral RNA cage.
Tang
L
L
Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
Johnson
K N
KN
Ball
L A
LA
Lin
T
T
Yeager
M
M
Johnson
J E
JE
D000595:Amino Acid Sequence; D000818:Animals; D002213:Capsid / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D020285:Cryoelectron Microscopy; D018360:Crystallography, X-Ray; D016679:Genome, Viral; D007304:Insect Viruses / Q000737:chemistry / Q000235:genetics* / Q000648:ultrastructure*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D021122:Protein Subunits; D012328:RNA Viruses / Q000737:chemistry / Q000235:genetics* / Q000648:ultrastructure*; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics / Q000648:ultrastructure*; D016415:Sequence Alignment; D018411:Spodoptera / Q000821:virology*
D021122:Protein Subunits; D012367:RNA, Viral