Overexpression of HSP70 prevents ultraviolet B-induced apoptosis of a human melanoma cell line.
The heat shock response is a highly conserved reaction common to all cells and organisms. It has been reported that hyperthermic treatment can induce the expression of the heat shock protein (HSP) and can protect cells from ultraviolet (UV) B radiation. In this study, we evaluated the effects of induced HSP70 on resistance to UV radiation. G361 amelanotic human melanoma cells were irradiated with increasing doses of UVB. UVB irradiation caused apoptotic cell death in these cells. Following transfection with MFG.hsp70.puro plasmid, the expression of HSP70 was determined. Compared to control vector-transfected cells, hsp70-transfected cells showed significantly elevated levels of HSP70 and were highly resistant to UVB irradiation. In order to investigate the effects of HSP70 on the apoptotic pathway, the changes in caspase-3 and PARP were analyzed. Following UVB irradiation, activation of caspase-3 and cleavage of PARP were observed in control vector-transfected cells, and the changes in these molecules were inhibited in the hsp70-transfected cells. These results suggest that UVB-induced apoptosis of melanoma cells is accompanied by caspase-3 activation and PARP cleavage, which can be prevented by an overexpression of HSP70.
Park
K C
KC
Department of Dermatology, Seoul National University College of Medicine, Seoul, Korea. gcpark@snu.ac.kr
Kim
D S
DS
Choi
H O
HO
Kim
K H
KH
Chung
J H
JH
Eun
H C
HC
Lee
J S
JS
Seo
J S
JS
D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D020169:Caspases / Q000032:analysis; D004307:Dose-Response Relationship, Radiation; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology*; D006801:Humans; D015151:Immunoblotting; D014162:Transfection; D014170:Transformation, Genetic; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays
D018840:HSP70 Heat-Shock Proteins; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases