Accelerated telomere shortening in hematological lineages is limited to the first year following stem cell transplantation.
Using quantitative fluorescence in situ hybridization and flow cytometry, the telomere length of telomere repeat sequences after stem cell transplantation (SCT) were measured. The study included the telomeres of peripheral blood monocytes that should reflect the length of telomeres in stem cells and the telomeres of T lymphocytes that could shorten as a result of peripheral expansion. The loss of telomeres in monocytes and in memory T cells, although accelerated initially, became comparable to the loss of telomeres in healthy controls from the second year after transplantation. In addition, the telomere length in the naive T cells that were produced by the thymus was comparable to the telomere length in the naive T cells of the donor. Compared to the total length of telomeres available, the loss of telomere repeats in leukocytes after SCT resembles the accelerated shortening seen in early childhood and remains, therefore, relatively insignificant.
Rufer
N
N
Division of Immunology and Allergology and the Division of Hematology and Oncology, University of Geneva, Geneva, Switzerland.
Brümmendorf
T H
TH
Chapuis
B
B
Helg
C
C
Lansdorp
P M
PM
Roosnek
E
E
D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000648:ultrastructure; D018929:Cell Culture Techniques; D002455:Cell Division / Q000235:genetics; D005500:Follow-Up Studies; D006410:Hematopoiesis / Q000235:genetics*; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007938:Leukemia / Q000097:blood / Q000628:therapy; D009000:Monocytes / Q000166:cytology / Q000648:ultrastructure; D013601:T-Lymphocytes / Q000166:cytology / Q000648:ultrastructure; D016615:Telomere / Q000502:physiology*; D013997:Time Factors