title: Noncovalent binding of poly(ADP-ribose) to nuclear matrix proteins: developmental changes and tissue specificity.
abstract: Poly(ADP-ribose) is a nuclear polynucleotide involved in the regulation of chromatin functions via covalent and/or noncovalent modification of nuclear proteins. Using a binding assay on protein blots, we searched for poly(ADP-ribose) binding proteins in nuclear matrices from testes of differently aged rats as well as from various adult rat tissues (brain, liver, spleen). We found that nuclear matrix proteins represent a significant subset of the nuclear proteins that can establish noncovalent interactions with poly(ADP-ribose). The profiles of poly(ADP-ribose) binding nuclear matrix proteins appeared to be tissue-specific and changed during postnatal development in the testis. The isolation and analysis of endogenous poly-(ADP-ribose) from rat testes showed that the ADP-ribose polymers that bind nuclear matrix proteins in vitro are also present under physiologic conditions in vivo. These results further substantiate the possibility that poly(ADP-ribose) may affect chromatin functions through noncovalent interaction with specific protein targets, including nuclear matrix components.
authors.affiliation: Department of Organic and Biological Chemistry, University of Naples Federico II, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Malanga
authors.forename: B
authors.initials: B
authors.lastname: Farina
mesh_terms: D000818:Animals; D034961:Antigens, Nuclear; D002467:Cell Nucleus / Q000378:metabolism; D008297:Male; D015530:Nuclear Matrix / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism*; D011064:Poly Adenosine Diphosphate Ribose / Q000378:metabolism*; D011108:Polymers; D051381:Rats; D017208:Rats, Wistar; D013737:Testis / Q000378:metabolism
chemical_list: D034961:Antigens, Nuclear; D009687:Nuclear Proteins; D011108:Polymers; D011064:Poly Adenosine Diphosphate Ribose