title: Intracellular distribution of GABA in the rat anterior pituitary. An electron microscopic autoradiographic study.
abstract: We studied the internalization and intracellular distribution of [3H] GABA in rat anterior pituitary cells. Electron microscopic autoradiography of anterior pituitary fragments or dispersed pituitary cells incubated with [3H] GABA showed that lactotrophs and, to a lesser extent, somatotrophs were the only cells that contained radioactive grains. Grain density analysis performed on dispersed pituitary cells after a pulse-chase experiment (10 min pulse and then change to a medium without radioactive GABA for various periods up to 2 h) revealed that GABA internalized by lactotrophs was distributed in various intracellular membranous organelles. Of the cell compartments examined, plasma membrane, Golgi apparatus, mitochondria and secretory granules had different time-dependent labeling patterns. The highest grain density values were associated with plasma membrane (at the first chase time) and the Golgi apparatus. Mitochondria and secretory granules also showed significant grain density values. A similar pattern of distribution was observed when fragments of prolactin-secreting pituitary adenomas were incubated with [3H] GABA. These results provide morphological data on the cellular specificity and intracellular distribution of GABA in anterior pituitary cells.
authors.affiliation: Centro de Investigaciones en Reproducción, Facultad de Medicina, Universidad de Buenos Aires, Argentina. duvilan@mail.retina.ar
authors.forename: B H
authors.initials: BH
authors.lastname: Duvilanski
authors.forename: R
authors.initials: R
authors.lastname: Pérez
authors.forename: A
authors.initials: A
authors.lastname: Seilicovich
authors.forename: M
authors.initials: M
authors.lastname: Lasaga
authors.forename: M C
authors.initials: MC
authors.lastname: Díaz
authors.forename: L
authors.initials: L
authors.lastname: Debeljuk
mesh_terms: D000818:Animals; D001345:Autoradiography; D002451:Cell Compartmentation / Q000187:drug effects / Q000502:physiology; D013006:Growth Hormone / Q000378:metabolism; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D010903:Pituitary Gland, Anterior / Q000378:metabolism* / Q000648:ultrastructure*; D011388:Prolactin / Q000378:metabolism; D015175:Prolactinoma / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D014316:Tritium; D005680:gamma-Aminobutyric Acid / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D014316:Tritium; D005680:gamma-Aminobutyric Acid; D011388:Prolactin; D013006:Growth Hormone