Fertile and diploid nuclear transplants derived from embryonic cells of a small laboratory fish, medaka (Oryzias latipes).
Fertile and diploid nuclear transplants were successfully generated by using embryonic cells as donors in a small laboratory fish, medaka (Oryzias latipes). Embryonic cell nuclei from transgenic fish carrying the green fluorescent protein (GFP) gene were transplanted into unfertilized eggs enucleated by x-ray irradiation. In this study, 1 out of 588 eggs transplanted in the first experiment and 5 out of 298 eggs transplanted in the second experiment reached the adult stage. All of these nuclear transplants were fertile and diploid, and the natural and GFP markers of the donor nuclei were transmitted to the F(1) and F(2) offspring in a Mendelian fashion. This systematic study proves the feasibility of generating nuclear transplants by using embryonic cells from fish as donors, and it is supported by convincing evidence.
Wakamatsu
Y
Y
Laboratory of Freshwater Fish Stocks, Bioscience Center, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan. wakamatu@fbio.nagoya-u.ac.jp
Ju
B
B
Pristyaznhyuk
I
I
Niwa
K
K
Ladygina
T
T
Kinoshita
M
M
Araki
K
K
Ozato
K
K
D000818:Animals; D030801:Animals, Genetically Modified; D000830:Animals, Laboratory; D001755:Blastocyst / Q000502:physiology*; D002467:Cell Nucleus / Q000502:physiology / Q000528:radiation effects; D002874:Chromosome Mapping; D004171:Diploidy; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000502:physiology*; D005260:Female; D005298:Fertility; D005819:Genetic Markers; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000032:analysis / Q000235:genetics; D009024:Morphogenesis; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects; D009990:Oryzias / Q000196:embryology* / Q000235:genetics; D011401:Promoter Regions, Genetic
D005819:Genetic Markers; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins