title: Trypanosoma cruzi epimastigote endocytic pathway: cargo enters the cytostome and passes through an early endosomal network before storage in reservosomes.
abstract: It has been known for many years that trypanosomatids require exogenous essential growth factors in order to divide. Two surface domains are involved in starting nutrient endocytosis: the flagellar pocket and the cytostome. Although the flagellar pocket plays a fundamental role in the endocytic process occurring in several trypanosomatids, we have shown the cytostome as the main structure involved in this process in epimastigote forms of T. cruzi. After one minute of endocytosis, cargo is still found at the cytostome entry as well as along the cytopharynx. After two, five and fifteen minutes of endocytosis, cargo was seen inside vesicles and tubules, prior to fusing with reservosomes. Three-dimensional reconstruction of these tubules and vesicles showed they are interconnected, forming an intricate and branched network, distributed from the perinuclear region to the posterior end of the cell. Whole unfixed parasites that had taken up gold-protein conjugates for fifteen minutes were washed and dried on electron microscope grids. Observation with an energy-filtering transmission electron microscope revealed long gold-filled tubules at the posterior end of the cell. Parasites treated with ammonium chloride had their intracellular traffic slowed down, which allowed us to observe many events of vesicle fusion. The acidic nature of this network was evidenced using acridine orange. Based on pH and protein uptake kinetics we propose that the vesicular-tubular network is the early endosome of Trypanosoma cruzi epimastigotes.
authors.affiliation: Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, UFRJ, Rio de Janeiro, Brasil.
authors.forename: I
authors.initials: I
authors.lastname: Porto-Carreiro
authors.forename: M
authors.initials: M
authors.lastname: Attias
authors.forename: K
authors.initials: K
authors.lastname: Miranda
authors.forename: W
authors.initials: W
authors.lastname: De Souza
authors.forename: N
authors.initials: N
authors.lastname: Cunha-e-Silva
mesh_terms: D000165:Acridine Orange / Q000378:metabolism; D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D004705:Endocytosis / Q000502:physiology*; D011992:Endosomes / Q000378:metabolism*; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D017979:Gold Colloid; D007091:Image Processing, Computer-Assisted; D007425:Intracellular Membranes / Q000378:metabolism; D008854:Microscopy, Electron; D008954:Models, Biological; D021381:Protein Transport; D012710:Serum Albumin, Bovine / Q000378:metabolism; D022161:Transport Vesicles / Q000378:metabolism*; D014349:Trypanosoma cruzi / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D005456:Fluorescent Dyes; D017979:Gold Colloid; D000643:Ammonium Chloride; D012710:Serum Albumin, Bovine; D000165:Acridine Orange; D016650:Fluorescein-5-isothiocyanate