The isolation and properties of the dimeric subunit of concanavalin A.
Concanavalin A (Con A) was dissociated into dimeric and monomeric subunits by incubation at 37 degrees C in acetate buffer of pH 3.8 containing 0.5% sodium dodecyl sulfate. The dimer was isolated in pure form by a density gradient ultracentrifugation method. Several properties of the dimer were determined including the formation of a precipitin with anti-Con A antibodies, the molecular weight, the lack of a binding site for glycogen, the lack of mitogenic activity for spleen lymphocytes, and the lack of inhibition by alpha-methyl D-glucoside. The latter findings differ from results reported by other investigators.
Pazur
J H
JH
Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park 16802-4500, USA.
Perloff
M D
MD
Frymoyer
A R
AR
Jensen
C J
CJ
Micolochick
H
H
Mastro
A
A
D002846:Chromatography, Affinity; D003208:Concanavalin A / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D019281:Dimerization; D004591:Electrophoresis, Polyacrylamide Gel; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects; D008934:Mitogens / Q000494:pharmacology; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D014461:Ultracentrifugation
D008934:Mitogens; D003208:Concanavalin A