title: Transfer RNA-mediated editing in threonyl-tRNA synthetase. The class II solution to the double discrimination problem.
abstract: Threonyl-tRNA synthetase, a class II synthetase, uses a unique zinc ion to discriminate against the isosteric valine at the activation step. The crystal structure of the enzyme with an analog of seryl adenylate shows that the noncognate serine cannot be fully discriminated at that step. We show that hydrolysis of the incorrectly formed ser-tRNA(Thr) is performed at a specific site in the N-terminal domain of the enzyme. The present study suggests that both classes of synthetases use effectively the ability of the CCA end of tRNA to switch between a hairpin and a helical conformation for aminoacylation and editing. As a consequence, the editing mechanism of both classes of synthetases can be described as mirror images, as already seen for tRNA binding and amino acid activation.
authors.affiliation: UPR 9004 Biologie Structurale IGBMC, CNRS/INSERM/ULP BP163 67404 Cedex, Illkirch, France.
authors.forename: A
authors.initials: A
authors.lastname: Dock-Bregeon
authors.forename: R
authors.initials: R
authors.lastname: Sankaranarayanan
authors.forename: P
authors.initials: P
authors.lastname: Romby
authors.forename: J
authors.initials: J
authors.lastname: Caillet
authors.forename: M
authors.initials: M
authors.lastname: Springer
authors.forename: B
authors.initials: B
authors.lastname: Rees
authors.forename: C S
authors.initials: CS
authors.lastname: Francklyn
authors.forename: C
authors.initials: C
authors.lastname: Ehresmann
authors.forename: D
authors.initials: D
authors.lastname: Moras
mesh_terms: D000215:Acylation; D001665:Binding Sites; D018360:Crystallography, X-Ray; D007700:Kinetics; D008958:Models, Molecular; D009154:Mutation; D009690:Nucleic Acid Conformation; D017434:Protein Structure, Tertiary; D017393:RNA Editing; D012346:RNA, Transfer, Amino Acyl / Q000737:chemistry / Q000378:metabolism*; D012694:Serine / Q000378:metabolism; D013912:Threonine / Q000378:metabolism; D013914:Threonine-tRNA Ligase / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D046249:Transfer RNA Aminoacylation; D015032:Zinc / Q000378:metabolism
chemical_list: D012346:RNA, Transfer, Amino Acyl; D013912:Threonine; D012694:Serine; D013914:Threonine-tRNA Ligase; D015032:Zinc