Cellular processing of (125)I- and (111)in-labeled epidermal growth factor (EGF) bound to cultured A431 tumor cells.
Low molecular weight of epidermal growth factor (EGF) enables better intratumoral penetration in comparison with larger targeting proteins, but the cellular retention of EGF-associated radioactivity is poor for directly iodinated EGF. An attempt was made to improve intracellular retention by the use of metal-diethylenetriaminepentaacetic acid or nonphenolic linker (N-succinimidyl-para-iodobenzoate) as labeling agents. The use of nonphenolic linker did not improve retention of the radioactivity in A431 carcinoma cell line. The use of the radiometal label provided an appreciable prolongation of radioactivity residence inside the cell.
Orlova
A
A
Department of Biomedical Radiation Sciences, Rudbecklaboratoriet, Uppsala University, Uppsala, Sweden. Anna.Orlova@bms.uu.se
Bruskin
A
A
Sjöström
A
A
Lundqvist
H
H
Gedda
L
L
Tolmachev
V
V
D002614:Chelating Agents / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004815:Epidermal Growth Factor / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007205:Indium Radioisotopes; D007457:Iodine Radioisotopes; D007463:Iodobenzoates / Q000737:chemistry; D007553:Isotope Labeling; D007700:Kinetics; D004369:Pentetic Acid / Q000737:chemistry; D019275:Radiopharmaceuticals / Q000737:chemistry / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D002614:Chelating Agents; D007205:Indium Radioisotopes; D007457:Iodine Radioisotopes; D007463:Iodobenzoates; D019275:Radiopharmaceuticals; C062338:N-succinimidyl 4-iodobenzoate; D004815:Epidermal Growth Factor; D004369:Pentetic Acid