Activity of buforin II alone and in combination with azithromycin and minocycline against Cryptosporidium parvum in cell culture.
The in vitro anti-cryptosporidial activity of buforin II alone and in combination with azithromycin and minocycline was investigated. Buforin II showed moderate activity, which increased with increasing concentration to 55.7% suppression of growth at 20 microM. Moreover, its activity was enhanced when it was combined with either azithromycin or minocycline with >90% parasite reduction at the highest concentration tested. Buforin II may be active in inhibiting Cryptosporidium parvum growth in vitro upon combination with either azithromycin or minocycline.
Giacometti
A
A
Institute of Infectious Diseases and Public Health, University of Ancona, Ancona, Italy. cmalinf@popcsi.unian.it
Cirioni
O
O
Del Prete MS
Barchiesi
F
F
Fineo
A
A
Scalise
G
G
D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D017963:Azithromycin / Q000494:pharmacology*; D016785:Cryptosporidium parvum / Q000187:drug effects* / Q000254:growth & development; D004353:Drug Evaluation, Preclinical; D004347:Drug Interactions; D006801:Humans; D008911:Minocycline / Q000494:pharmacology*; D011506:Proteins / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000890:Anti-Infective Agents; D011506:Proteins; C097849:buforin II; D017963:Azithromycin; D008911:Minocycline