title: Induction of immune responses in newborn lambs following enteric immunization with a human adenovirus vaccine vector.
abstract: We investigated the antigen-specific mucosal and systemic immune responses of newborn lambs following enteric immunization, targeting jejunal Peyer's patches with a human adenovirus vector that expressed the glycoprotein D (gD) of bovine herpesvirus-1. Both humoral and cell-mediated gD-specific mucosal immune responses were detected in newborn lambs (1-4 days old) after a single immunization and these responses were qualitatively and quantitatively similar to those detected in 5-6-week-old lambs. Passively transferred gD-specific maternal antibody did not significantly alter either mucosal or systemic gD-specific immune responses. Furthermore, enteric immunization of newborn lambs primed mucosal immune responses in the lungs. These observations confirmed that gut-associated lymphoid tissue of a newborn ruminant is immune competent and that enteric immunization may be an effective approach for the induction of both mucosal and systemic immune responses in the neonate.
authors.affiliation: Veterinary Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, S7N 5E3, Saskatoon, Sask., Canada.
authors.forename: G
authors.initials: G
authors.lastname: Mutwiri
authors.forename: C
authors.initials: C
authors.lastname: Bateman
authors.forename: M E
authors.initials: ME
authors.lastname: Baca-Estrada
authors.forename: M
authors.initials: M
authors.lastname: Snider
authors.forename: P
authors.initials: P
authors.lastname: Griebel
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000831:Animals, Newborn; D002417:Cattle; D003126:Colostrum / Q000276:immunology; D005822:Genetic Vectors; D006801:Humans; D018928:Immunity, Mucosal; D007114:Immunization; D007121:Immunocompetence; D007371:Interferon-gamma / Q000378:metabolism; D007413:Intestinal Mucosa / Q000276:immunology*; D008221:Lymphoid Tissue / Q000276:immunology; D012756:Sheep; D014764:Viral Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D014764:Viral Proteins; D014765:Viral Vaccines; C046998:bovine herpesvirus type-1 glycoproteins; D007371:Interferon-gamma