title: Genomic exploration of the hemiascomycetous yeasts: 2. Data generation and processing.
abstract: The generation of sequencing data for the hemiascomycetous yeast random sequence tag project was performed using the procedures established at GENOSCOPE. These procedures include a series of protocols for the sequencing reactions, using infra-red labelled primers, performed on both ends of the plasmid inserts in the same reaction tube, and their analysis on automated DNA sequencers. They also include a package of computer programs aimed at detecting potential assignation errors, selecting good quality sequences and estimating their useful length.
authors.affiliation: GENOSCOPE, Centre National de Séquençage, 2 rue Gaston Crémieux, P.O. Box 91, Cedex, F-Evry, France. artigue@genoscope.cns.fr
authors.forename: F
authors.initials: F
authors.lastname: Artiguenave
authors.forename: P
authors.initials: P
authors.lastname: Wincker
authors.forename: P
authors.initials: P
authors.lastname: Brottier
authors.forename: S
authors.initials: S
authors.lastname: Duprat
authors.forename: F
authors.initials: F
authors.lastname: Jovelin
authors.forename: C
authors.initials: C
authors.lastname: Scarpelli
authors.forename: J
authors.initials: J
authors.lastname: Verdier
authors.forename: V
authors.initials: V
authors.lastname: Vico
authors.forename: J
authors.initials: J
authors.lastname: Weissenbach
authors.forename: W
authors.initials: W
authors.lastname: Saurin
mesh_terms: D001203:Ascomycota / Q000235:genetics*; D019295:Computational Biology / Q000379:methods*; D016681:Genome, Fungal; D010843:Pichia / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods*