Transmission electron microscopy of the interface between bone and pseudowollastonite implant.
This paper reports on the structural morphology of the interface in vivo between implants composed of bioactive synthetic pseudowollastonite ceramic and bone in rat tibias. Thin sections of the interfaces were examined after 6 and 8 weeks of implantation period in a high resolution transmission electron microscope up to the lattice plane resolution level. The interfaces developed normal biological and chemical activities and remained reactive over the 8-week period. The regions showing direct bone tissue bonding to the implant contained nanocrystals of hydroxyapatite-like phase growing epitaxially across the interface in the [002] direction. The nanocrystals were also identified in the bone tissue formed in the interfacial area. The reactivity of the implant caused in the first instance formation of an amorphous woven type of bone, which transformed into a crystalline lamellar type containing collagen fibres. The Ca/P ratio of the interfacial region was found to be between 1.67 in the mature bone tissue formed about 5 microm from the interface, and 2.06 in the regions right at the interface.
De Aza
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PN
Departamento de Ciencia y Tecnologia de Materiales, Universidad Miguel Hernandez de Elche, Alicante, Spain. piedad@umh.es
Luklinska
Z B
ZB
Anseau
M R
MR
Guitian
F
F
De Aza
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S
D000818:Animals; D018786:Bone Substitutes; D001842:Bone and Bones / Q000378:metabolism / Q000648:ultrastructure*; D002118:Calcium / Q000032:analysis; D017610:Calcium Compounds; D003094:Collagen / Q000032:analysis; D003460:Crystallization; D017886:Durapatite / Q000032:analysis; D020007:Implants, Experimental; D008297:Male; D008854:Microscopy, Electron; D010758:Phosphorus / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D017640:Silicates; D013499:Surface Properties; D013997:Time Factors
D018786:Bone Substitutes; D017610:Calcium Compounds; D017640:Silicates; D010758:Phosphorus; D003094:Collagen; D017886:Durapatite; C031293:calcium silicate; D002118:Calcium