title: Mass rearing the weevil Hylobius transversovittatus (Coleoptera: Curculionidae), biological control agent of Lythrum salicaria, on semiartificial diet.
abstract: Lythrum salicaria L., purple loosestrife, an invasive Eurasian perennial, is degrading wetlands across temperate North America. Chemical, physical, and mechanical methods failed to provide long-term control. Therefore, four host-specific insect species (two leaf feeders, a root feeder, and a flower feeder) were introduced as biological control agents. To increase the availability of adults of the root feeding weevil Hylobius transversovittatus Goeze for field releases, we developed a semiartificial diet. Suitability of different diet formulations (varying vitamin mixes, salt mixes, antimicrobials, water content, root content) and temperatures for larval development were evaluated. We also monitored how rearing on artificial diet and the number of larvae per container affected larval development time, larval survival, adult weight, and incidences of deformities. Rearing larvae on artificial diet reduced development time from 1-2 yr to 2-3 mo. Larval development was fastest and survival rates highest under constant temperatures of 25 degrees C. Hatch rate and larval survival decreased if eggs were surface sterilized. Using FABCO antimicrobials could not prevent fungal contamination; use of methyl paraben and sorbic acid was successful in suppressing fungal and bacterial growth throughout larval development time to <10%. The moisture content of the diet did not significantly affect larval survival, development, or adult weight. Decreasing the proportion of purple loosestrife roots in the diet reduced adult weight and the proportion of larvae completing development, and increased development time; no larvae were able to complete development in root-free diet. With an increase in the number of larvae per cup, survival rates were reduced. The experiments revealed a female biased sex ratio: females consistently developed faster and were heavier than males. Incidence of adult deformities was consistently below 5%. Increased availability of adults for field release as a result of mass production using the semiartificial diet will accelerate the biocontrol program targeting purple loosestrife. We are able to produce several hundred weevils per week and have adapted the diet to rear other root-feeding weevils.
authors.affiliation: Department of Natural Resources, Cornell University, Ithaca, NY 14853, USA.
authors.forename: B
authors.initials: B
authors.lastname: Blossey
authors.forename: D
authors.initials: D
authors.lastname: Eberts
authors.forename: E
authors.initials: E
authors.lastname: Morrison
authors.forename: T R
authors.initials: TR
authors.lastname: Hunt
mesh_terms: D000818:Animals; D001517:Coleoptera / Q000254:growth & development*; D004032:Diet; D005260:Female; D007814:Larva / Q000254:growth & development; D010058:Oviposition; D010572:Pest Control, Biological; D010944:Plants; D013696:Temperature; D014815:Vitamins
chemical_list: D014815:Vitamins