title: Limited genetic diversity of Brucella spp.
abstract: Multilocus enzyme electrophoresis (MLEE) of 99 Brucella isolates, including the type strains from all recognized species, revealed a very limited genetic diversity and supports the proposal of a monospecific genus. In MLEE-derived dendrograms, Brucella abortus and a marine Brucella sp. grouped into a single electrophoretic type related to Brucella neotomae and Brucella ovis. Brucella suis and Brucella canis formed another cluster linked to Brucella melitensis and related to Rhizobium tropici. The Brucella strains tested that were representatives of the six electrophoretic types had the same rRNA gene restriction fragment length polymorphism patterns and identical ribotypes. All 99 isolates had similar chromosome profiles as revealed by the Eckhardt procedure.
authors.affiliation: Escuela Nacional de Ciencias Biológicas, IPN, Zacatecas, Mexico.
authors.forename: B
authors.initials: B
authors.lastname: Gándara
authors.forename: A L
authors.initials: AL
authors.lastname: Merino
authors.forename: M A
authors.initials: MA
authors.lastname: Rogel
authors.forename: E
authors.initials: E
authors.lastname: Martínez-Romero
mesh_terms: D000818:Animals; D002002:Brucella / Q000145:classification* / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D002006:Brucellosis / Q000382:microbiology*; D002417:Cattle; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D003611:Dairy Products / Q000382:microbiology*; D004285:Dogs; D004592:Electrophoresis, Starch Gel / Q000379:methods; D004798:Enzymes / Q000032:analysis; D014644:Genetic Variation; D006801:Humans; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D021521:Ribotyping
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D004798:Enzymes; D012336:RNA, Ribosomal, 16S