The AIDA autotransporter system is associated with F18 and stx2e in Escherichia coli isolates from pigs diagnosed with edema disease and postweaning diarrhea.
Pathogenic Escherichia coli strains are known to cause edema disease (ED) and postweaning diarrhea (PWD) in piglets. Although the exact mechanisms of pathogenicity that lead to ED-PWD remain to be elucidated, E. coli-borne Shiga-like toxin and adhesion-mediating virulence factors such as F18 adhesin or F4 fimbriae are believed to play a central role in ED-PWD. In light of these observations we investigated whether another E. coli adhesin, the plasmid-encoded AIDA (adhesin involved in diffuse adherence) might also be present in ED-PWD-causing E. coli isolates. For rapid screening for the AIDA system in large numbers of isolates, a multiplex PCR method along with a duplex Western blot procedure was developed. When screening 104 strains obtained from pigs with or without ED-PWD, we observed a high prevalence of the AIDA operon in porcine E. coli isolates, with over 25% of all strains being AIDA positive, and we could demonstrate a significant association of the intact AIDA gene (orfB) with ED-PWD, while defects in orfB were associated with the absence of disease. Although our data hint toward a contribution of AIDA to ED-PWD, further studies will be necessary since the presence of the AIDA genes was also associated with the presence of the Shiga-like toxin and F18 adhesin genes, two reported virulence factors for ED-PWD.
Niewerth
U
U
Institut für Infektiologie, Zentrum für Molekularbiologie der Entzündung, Westfälische Wilhelms-Universität, Münster, Germany.
Frey
A
A
Voss
T
T
Le Bouguénec
C
C
Baljer
G
G
Franke
S
S
Schmidt
M A
MA
D018830:Adhesins, Escherichia coli / Q000235:genetics*; D000818:Animals; D000942:Antigens, Bacterial; D001426:Bacterial Proteins / Q000235:genetics*; D015153:Blotting, Western / Q000379:methods; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D004488:Edema Disease of Swine / Q000382:microbiology*; D004926:Escherichia coli / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D029968:Escherichia coli Proteins; D036781:Fimbriae Proteins; D005798:Genes, Bacterial; D008040:Genetic Linkage; D014644:Genetic Variation; D009876:Operon; D016133:Polymerase Chain Reaction / Q000379:methods; D012703:Serotyping; D022641:Shiga Toxin 2 / Q000235:genetics*; D013552:Swine; D013997:Time Factors
C075559:AIDA-I protein, E coli; D018830:Adhesins, Escherichia coli; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D022641:Shiga Toxin 2; C080952:DaaE protein, E coli; D036781:Fimbriae Proteins