title: Thermal unfolding of a llama antibody fragment: a two-state reversible process.
abstract: Camelids produce functional "heavy chain" antibodies which are devoid of light chains and CH1 domains [Hamers-Casterman, C., et al. (1993) Nature 363, 446-448]. It has been shown that the variable domains of these heavy chain antibodies (the V(HH) fragments) are functional at or after exposure to high temperatures, in contrast to conventional antibodies [Linden van der, R. H. J., et al. (1999) Biochim. Biophys. Acta 1431, 37-44]. For a detailed understanding of the higher thermostability of these V(HH) fragments, knowledge of their structure and conformational dynamics is required. As a first step toward this goal, we report here the essentially complete (1)H and (15)N NMR backbone resonance assignments of a llama V(HH) antibody fragment, and an extensive analysis of the structure at higher temperatures. The H-D exchange NMR data at 300 K indicate that the framework of the llama V(HH) fragment is highly protected with a DeltaG(ex) of >5.4 kcal/mol, while more flexibility is observed for surface residues, particularly in the loops and the two outer strands (residues 4-7, 10-13, and 58-60) of the beta-sheet. The CD data indicate a reversible, two-state unfolding mechanism with a melting transition at 333 K and a DeltaH(m) of 56 kcal/mol. H-D exchange studies using NMR and ESI-MS show that below 313 K exchange occurs through local unfolding events whereas above 333 K exchange mainly occurs through global unfolding. The lack of a stable core at high temperatures, observed for V(HH) fragments, has also been observed for conventional antibody fragments. The main distinction between the llama V(HH) fragment and conventional antibody fragments is the reversibility of the thermal unfolding process, explaining its retained functionality after exposure to high temperatures.
authors.affiliation: Unilever Research, Olivier van Noortlaan 120, 3133 AT Vlaardingen, The Netherlands.
authors.forename: J M
authors.initials: JM
authors.lastname: Pérez
authors.forename: J G
authors.initials: JG
authors.lastname: Renisio
authors.forename: J J
authors.initials: JJ
authors.lastname: Prompers
authors.forename: C J
authors.initials: CJ
authors.lastname: van Platerink
authors.forename: C
authors.initials: C
authors.lastname: Cambillau
authors.forename: H
authors.initials: H
authors.lastname: Darbon
authors.forename: L G
authors.initials: LG
authors.lastname: Frenken
mesh_terms: D000577:Amides; D000818:Animals; D002161:Camelids, New World / Q000276:immunology*; D006063:Chorionic Gonadotropin / Q000235:genetics / Q000276:immunology; D002942:Circular Dichroism; D003903:Deuterium; D006358:Hot Temperature; D006801:Humans; D006859:Hydrogen; D007143:Immunoglobulin Heavy Chains / Q000737:chemistry* / Q000378:metabolism; D007135:Immunoglobulin Variable Region / Q000737:chemistry* / Q000378:metabolism; D013058:Mass Spectrometry; D019906:Nuclear Magnetic Resonance, Biomolecular; D011489:Protein Denaturation; D017510:Protein Folding; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D013816:Thermodynamics
chemical_list: D000577:Amides; D006063:Chorionic Gonadotropin; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D011994:Recombinant Proteins; D006859:Hydrogen; D003903:Deuterium