title: Functional tethered lipid bilayers.
abstract: Our strategy to provide the structural basis for the build-up of functional tethered membranes focuses on three approaches: the first one is based on the pre-organization of a monomolecular layer of a lipopolymer at the water/air interface which is then transferred to a solid support. Prior to deposition, the substrate is coated with a layer of benzophenone-derivatized silane molecules that allow for a stable covalent attachment by photo-cross-linking of some of the monomer units of the lipopolymer to the support. An alternative concept realizes a layer-by-layer deposition of the various structural elements: (1) the attachment layer with the reactive sites for the chemical stabilization; (2) a polymer 'cushion' prepared by adsorption and simultaneous or subsequent partial covalent binding to the reactive sites; and (3) a lipid monolayer transferred from the water/air interface, that contains a certain amount of lipids with reactive headgroups which, upon binding to the polymer tether, act as anchor lipids stabilizing the whole monolayer/cushion-composite. And finally, we build peptide-supported monolayers by first (self-) assembling amino acid sequences of various lengths via a SH-group near their N-terminus onto Au substances and use then their COO(-)-terminus to chemically attach phosphatidyl-ethanolamine lipids to form a stable monolayer of lipid-peptide conjugates. All the individual preparation steps and the various resulting (multi-) layers are characterized by surface plasmon spectroscopy, X-ray and neutron-reflectometry, contact angle measurements, IR spectroscopy, fluorescence microscopy, scanning probe microscopies, as well as, electrochemical techniques. For all tethering systems, the final membranes' architecture is obtained by fusing lipid vesicles onto the lipid monolayer. Proteins can be incorporated by either fusing vesicles that are loaded with the respective receptors, pores, or ion pumps via a reconstitution procedure, or via a transfer directly from a micellar solution to the pre-formed lipid bilayer at the solid support by a dialysis step. Two structural/dynamical features of tethered membranes which are considered to be of particular functional relevance, i.e. the degree of water uptake and, hence, the degree of swelling of the polymer support, as well as the lateral mobility of the lipid molecules in the membrane, are tested by surface plasmon optics and by measurements of the fluorescence recovery after photobleaching (FRAP), respectively. The results confirm that the presented preparation protocols yield fluid bilayers that mimic certain relevant properties of biological membranes. The functional characterization of tethered membranes, which is briefly summarized, is based on various electrochemical techniques, in particular, impedance spectroscopy, cyclic voltammetry, and chronoamperometric studies. The results obtained for reconstituted H(+)-ATPase from chloroplasts and E. coli and for cytochrome oxidase (with and without cytochrome c) confirm the incorporation of the proteins in an active form, thus, opening opportunities for novel sensor formats or offering a completely new model membrane system.
authors.affiliation: Max-Planck-Institut für Polymerforschung, Ackermannweg 10, 55128 Mainz, Germany. knoll@mpip-mainz.mpg.de
authors.forename: W
authors.initials: W
authors.lastname: Knoll
authors.forename: C W
authors.initials: CW
authors.lastname: Frank
authors.forename: C
authors.initials: C
authors.lastname: Heibel
authors.forename: R
authors.initials: R
authors.lastname: Naumann
authors.forename: A
authors.initials: A
authors.lastname: Offenhäusser
authors.forename: J
authors.initials: J
authors.lastname: Rühe
authors.forename: E K
authors.initials: EK
authors.lastname: Schmidt
authors.forename: W W
authors.initials: WW
authors.lastname: Shen
authors.forename: A
authors.initials: A
authors.lastname: Sinner
mesh_terms: D001704:Biopolymers; D008051:Lipid Bilayers; D008565:Membrane Proteins / Q000737:chemistry; D008567:Membranes, Artificial; D008954:Models, Biological; D017494:Proton Pumps / Q000737:chemistry
chemical_list: D001704:Biopolymers; D008051:Lipid Bilayers; D008565:Membrane Proteins; D008567:Membranes, Artificial; D017494:Proton Pumps