Effects of cytokines on Porphyromonas gingivalis-induced opsonophagocytosis of a murine macrophage cell line.
A murine macrophage cell line was incubated with opsonized Porphyromonas gingivalis and various concentrations of rIFN-gamma, rIL-4 and rIL-10. The number of phagocyted cells were microscopically assessed. The results showed that IFN-gamma upregulated macrophage phagocytosis to this periodontopathogen, and the upregulatory effects was abolished by anti-IFN-gamma antibodies. Neither IL-4 nor IL-10 had any effects on opsonophagocytosis by this cell line. These results suggest that up-regulatory functions of IFN-gamma on phagocytic activities of macrophages may play a crucial role in the induction of immune response to P. gingivalis.
Sosroseno
W
W
Department of Oral Biology, Faculty of Dentistry, Gadjah Mada University, Yogyakarta, Indonesia. wihaskoro@kb.usm.my
Herminajeng
E
E
Herwiyanti
S
S
Ghufron
M
M
D000818:Animals; D000906:Antibodies / Q000276:immunology; D002460:Cell Line; D016207:Cytokines / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology; D016753:Interleukin-10 / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology; D008264:Macrophages / Q000276:immunology / Q000502:physiology*; D051379:Mice; D009895:Opsonin Proteins / Q000276:immunology*; D010587:Phagocytosis / Q000276:immunology / Q000502:physiology*; D016966:Porphyromonas gingivalis / Q000276:immunology / Q000502:physiology*; D015854:Up-Regulation / Q000276:immunology
D000906:Antibodies; D016207:Cytokines; D009895:Opsonin Proteins; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma