Preliminary 1H NMR investigation of sialic acid transfer by the trans-sialidase from Trypanosoma cruzi.
1H NMR spectroscopy has been used to investigate the transfer of sialic acid from sialic acid donor molecules to acceptor molecules using the trans-sialidase from Typanosoma cruzi. It is clearly demonstrated that NMR spectroscopy is an efficient and powerful means of monitoring the trans-sialidase promoted transfer of sialic acid from donor to acceptor.
Wilson
J C
JC
Centre for Biomolecular Science and Drug Discovery, Griffith University, Queensland, Australia.
Kiefel
M J
MJ
Albouz-Abo
S
S
von Itzstein
M
M
D000606:Amino Sugars / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D014355:Chagas Disease / Q000201:enzymology / Q000209:etiology / Q000469:parasitology; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D007785:Lactose / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D019158:N-Acetylneuraminic Acid / Q000737:chemistry / Q000378:metabolism*; D009439:Neuraminidase / Q000737:chemistry / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular; D011522:Protons; D012794:Sialic Acids / Q000737:chemistry / Q000378:metabolism; D012799:Sialyltransferases / Q000737:chemistry / Q000378:metabolism*; D014349:Trypanosoma cruzi / Q000201:enzymology*
D000606:Amino Sugars; D006023:Glycoproteins; D011522:Protons; D012794:Sialic Acids; C000020:N-acetylneuraminoyllactose; C000458:N-acetyllactosamine; D012799:Sialyltransferases; C069715:trans-sialidase; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid; D007785:Lactose