Early changes in quasispecies repertoire in HIV-infected infants: correlation with disease progression.
The evolution of HIV-1 quasispecies in patients during the first year of life was investigated in 10 vertically infected infants, using heteroduplex analysis of the V3-V5 region of env. Four subjects, who showed little viral evolution during the period of the study, had rapid progression of disease and early loss of CD4(+) cells. The remaining six subjects, who were slow progressors, evolved new viral variants within 6 months, and in one case by 1 month of age. Of the four patients who were PCR positive at birth, one was infected with multiple HIV-1 variants. These results show that in HIV-infected children, multiple variants may initiate infection and early quasispecies diversification is associated with a favorable clinical outcome.
Essajee
S M
SM
Department of Pediatric Infectious Diseases, New York University Medical Center, New York City, New York 10016, USA. shaffiq.essajee@med.nyu.edu
Pollack
H
H
Rochford
G
G
Oransky
I
I
Krasinski
K
K
Borkowsky
W
W
D018450:Disease Progression; D019143:Evolution, Molecular; D015686:Gene Products, env / Q000235:genetics; D015752:Genes, env; D014644:Genetic Variation; D015699:HIV Envelope Protein gp120 / Q000235:genetics; D015658:HIV Infections / Q000503:physiopathology* / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000235:genetics*; D020180:Heteroduplex Analysis; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D010446:Peptide Fragments / Q000235:genetics; D016133:Polymerase Chain Reaction
D015686:Gene Products, env; D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D010446:Peptide Fragments