title: Inhibition of the allograft response by donor specific blood transfusion: association with reduced local TH1 cytokines and nitric oxide but enhanced prostaglandin E2 production.
abstract: BACKGROUND
  Donor-specific blood transfusion (DST) may improve allograft survival in human and animal models, but the mechanisms for this graft protective effect are incompletely understood. The sponge matrix allograft model was used to determine if DST induces regulatory factors within the allograft.
  
  
  METHODS
  C57BL/6 (H-2b) recipients received donor-specific (DBA/2J, H-2d) or syngeneic (C57BL/6) blood 7 days before sponge matrix allograft (DBA/2J) implantation. Fourteen days postgrafting, the sponge infiltrating cells (SIC) were examined for cytotoxic T cell (CTL) and natural killer (NK) activity, and sponge exudate fluid (SEF) was assessed for nitric oxide (.N=O) and prostaglandin E2 (PGE2) content. Interleukin- (IL) 2, IL-4, IL-10, and interferon-gamma (IFN-gamma) production by SIC was also determined. Recipient splenocytes were simultaneously assessed for anti-donor cytotoxic and proliferative responses and .N=O production.
  
  
  RESULTS
  SIC from mice receiving syngeneic transfusions (ST) acquired both CTL and NK activity postgrafting, with maximal activity by day 14. DST suppressed both CTL and NK activity throughout the postgrafting period. Limiting dilution analysis (LDA) of SIC to determine precursor and native CTL frequency showed significantly lower responder cell frequency after DST compared with ST. SEF .N=O levels and SIC production of IL-2 and IFN-gamma in grafted DST mice were significantly lower than in grafted mice receiving ST. No significant amounts of IL-4 and very low levels of IL-10 were produced by SIC from grafted mice after either ST or DST. Conversely, PGE2 content of sponge fluid and serum from DST mice was higher than in mice receiving ST. Antigen stimulated splenocyte proliferation and CTL development assessed by LDA were also inhibited by DST.
  
  
  CONCLUSIONS
  Reduction in local TH1 cytokines, absence of detectable TH2 cytokines, with enhanced PGE2 and depressed .N=O were observed in the local graft environment after DST. These data support the hypothesis that DST induces donor-specific intragraft suppressor factors, accompanied by reduced local and systemic immune activation.
authors.affiliation: Department of Urology, University of Pittsburgh Medical Center and Veterans Administration Medical Center, PA 15213, USA.
authors.forename: S
authors.initials: S
authors.lastname: Koga
authors.forename: P P
authors.initials: PP
authors.lastname: Luke
authors.forename: S M
authors.initials: SM
authors.lastname: Specht
authors.forename: B
authors.initials: B
authors.lastname: Rominski
authors.forename: A
authors.initials: A
authors.lastname: Jaquins-Gerstl
authors.forename: R A
authors.initials: RA
authors.lastname: Hoffman
authors.forename: A W
authors.initials: AW
authors.lastname: Thomson
authors.forename: M L
authors.initials: ML
authors.lastname: Jordan
mesh_terms: D000818:Animals; D001803:Blood Transfusion; D016207:Cytokines / Q000096:biosynthesis*; D003602:Cytotoxicity, Immunologic; D015232:Dinoprostone / Q000096:biosynthesis*; D005260:Female; D006084:Graft Rejection / Q000276:immunology / Q000378:metabolism / Q000517:prevention & control*; D006801:Humans; D066298:In Vitro Techniques; D007694:Killer Cells, Natural / Q000276:immunology; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009569:Nitric Oxide / Q000378:metabolism*; D018417:Th1 Cells / Q000276:immunology*; D013997:Time Factors; D014019:Tissue Donors; D014184:Transplantation, Homologous; D014185:Transplantation, Isogeneic
chemical_list: D016207:Cytokines; D009569:Nitric Oxide; D015232:Dinoprostone