title: Diagnosis of granulocytic ehrlichiosis in humans by immunofluorescence assay.
abstract: Serodiagnostic tests are widely available for tick-borne diseases. We evaluated a cell-free antigen of the human granulocytic ehrlichiosis agent. Immunofluorescence assay (IFA) with this antigen is as efficient as with the MRL kit and allows a one-step IFA with other cell-free antigens that is useful when testing sera from patients bitten by ticks.
authors.affiliation: Unité des rickettsies, WHO Collaborative Center for Rickettsial Reference and Research, CNRS UPRES A 6020, Faculté de Médecine, 13385 Marseille Cedex 5 France. Philippe.Brouqui@medecine.univ-mrs.fr
authors.forename: P
authors.initials: P
authors.lastname: Brouqui
authors.forename: E
authors.initials: E
authors.lastname: Salvo
authors.forename: J S
authors.initials: JS
authors.lastname: Dumler
authors.forename: D
authors.initials: D
authors.lastname: Raoult
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D004536:Ehrlichia / Q000276:immunology; D016873:Ehrlichiosis / Q000097:blood / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D005455:Fluorescent Antibody Technique; D006098:Granulocytes; D018922:HL-60 Cells; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007075:Immunoglobulin M / Q000097:blood / Q000276:immunology; D011237:Predictive Value of Tests; D012698:Serologic Tests
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M