Expression of myeloperoxidase in the inner ear of cisplatin-treated guinea pigs.
Cisplatin is known to cause inner ear damage (ototoxicity). The role of myeloperoxidase (MPO) in the inner ear of the guinea pigs after injections of cisplatin i.p. was examined immunohistochemically. Three days after the injection, the animals were sacrificed with intracardiac perfusion of fixative, and temporal bones were removed and processed for immunohistochemistry with the anti-MPO antibody. MPO could be detected after 3 days in the lateral wall, the organ of Corti, supporting cells of the sensory epithelium and dark cells. These results suggest that MPO and reactive oxygen species are involved in the inner ear dysfunction after the application of cisplatin.
Watanabe
K
K
Department of Oto-Rhino-Laryngology, Nippon Medical School, Tokyo, Japan. bxp02646@nifty.ne.jp
Yagi
T
T
D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D002945:Cisplatin / Q000633:toxicity*; D007758:Ear, Inner / Q000187:drug effects* / Q000201:enzymology*; D004848:Epithelium / Q000187:drug effects / Q000201:enzymology; D006168:Guinea Pigs; D007150:Immunohistochemistry; D009195:Peroxidase / Q000096:biosynthesis*
D000970:Antineoplastic Agents; D009195:Peroxidase; D002945:Cisplatin