title: Inhaled concentrated ambient particles are associated with hematologic and bronchoalveolar lavage changes in canines.
abstract: Pulmonary inflammatory and hematologic responses of canines were studied after exposure to concentrated ambient particles (CAPs) using the Harvard ambient particle concentrator (HAPC). For pulmonary inflammatory studies, normal dogs were exposed in pairs to either CAPs or filtered air (paired studies) for 6 hr/day on 3 consecutive days. For hematologic studies, dogs were exposed for 6 hr/day for 3 consecutive days with one receiving CAPs while the other was simultaneously exposed to filtered air; crossover of exposure took place the following week (crossover studies). Physicochemical characterization of CAPs exposure samples included measurements of particle mass, size distribution, and composition. No statistical differences in biologic responses were found when all CAPs and all sham exposures were compared. However, the variability in biologic response was considerably higher with CAPs exposure. Subsequent exploratory graphical analyses and mixed linear regression analyses suggested associations between CAPs constituents and biologic responses. Factor analysis was applied to the compositional data from paired and crossover experiments to determine elements consistently associated with each other in CAPs samples. In paired experiments, four factors were identified; in crossover studies, a total of six factors were observed. Bronchoalveolar lavage (BAL) and hematologic data were regressed on the factor scores. Increased BAL neutrophil percentage, total peripheral white blood cell (WBC) counts, circulating neutrophils, and circulating lymphocytes were associated with increases in the aluminum/silicon factor. Increased circulating neutrophils and increased BAL macrophages were associated with the vanadium/nickel factor. Increased BAL neutrophils were associated with the bromine/lead factor when only the compositional data from the third day of CAPs exposure were used. Significant decreases in red blood cell counts and hemoglobin levels were correlated with the sulfur factor. BAL or hematologic parameters were not associated with increases in total CAPs mass concentration. These data suggest that CAPs inhalation is associated with subtle alterations in pulmonary and systemic cell profiles, and specific components of CAPs may be responsible for these biologic responses.
authors.affiliation: Department of Environmental Health, Harvard School of Public Health, Boston, MA 02115, USA. Robert.Clarke@alkermes.com
authors.forename: R W
authors.initials: RW
authors.lastname: Clarke
authors.forename: B
authors.initials: B
authors.lastname: Coull
authors.forename: U
authors.initials: U
authors.lastname: Reinisch
authors.forename: P
authors.initials: P
authors.lastname: Catalano
authors.forename: C R
authors.initials: CR
authors.lastname: Killingsworth
authors.forename: P
authors.initials: P
authors.lastname: Koutrakis
authors.forename: I
authors.initials: I
authors.lastname: Kavouras
authors.forename: G G
authors.initials: GG
authors.lastname: Murthy
authors.forename: J
authors.initials: J
authors.lastname: Lawrence
authors.forename: E
authors.initials: E
authors.lastname: Lovett
authors.forename: J M
authors.initials: JM
authors.lastname: Wolfson
authors.forename: R L
authors.initials: RL
authors.lastname: Verrier
authors.forename: J J
authors.initials: JJ
authors.lastname: Godleski
mesh_terms: D000393:Air Pollutants / Q000009:adverse effects*; D000818:Animals; D018893:Bronchoalveolar Lavage; D004285:Dogs; D005260:Female; D007111:Immunity, Cellular / Q000187:drug effects; D019570:Inhalation Exposure; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology*; D010316:Particle Size
chemical_list: D000393:Air Pollutants