Antinociceptive effect induced by intraperitoneal administration of vitamin K2 (menatetrenone) in ICR mice.
The antinociceptive effect of vitamin K2 (menatetrenone) in mice was examined using tail-flick and formalin test. Menatetrenone at doses of 10, 50 and 100 mg/kg, i.p. produced a dose-dependent and significant inhibition of the tail-flick response in mice. Menatetrenone (50 and 100 mg/kg, i.p.) had no significant effect on the duration of the first phase of the formalin-induced flinching. However, menatetrenone (100 mg/kg, i.p.) significantly inhibited the second phase of the formalin-induced flinching. I.p. administration of menatetrenone (100 mg/kg) significantly reduced the duration of nociceptive responses induced by i.t. injection of bradykinin, but not of substance P, prostaglandin E2 or N-methyl-D-aspartate (NMDA). These present data suggest that i.p. pretreatment with menatetrenone produced dose-dependent antinociceptive effect in mice. This effect may be, at least in part, mediated by the inhibition of bradykinin dependent nociceptive transmission in the spinal cord.
Onodera
K
K
Department of Pharmacology, School of Dentistry, Tohoku University, Sendai, Japan. kenjo@mail.cc.tohoku.ac.jp
Shinoda
H
H
Zushida
K
K
Taki
K
K
Kamei
J
J
D000700:Analgesics / Q000494:pharmacology*; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001920:Bradykinin / Q000008:administration & dosage / Q000494:pharmacology; D015232:Dinoprostone / Q000008:administration & dosage / Q000494:pharmacology; D007274:Injections, Intraperitoneal; D007278:Injections, Spinal; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D016202:N-Methylaspartate / Q000008:administration & dosage / Q000494:pharmacology; D013373:Substance P / Q000008:administration & dosage / Q000494:pharmacology; D014812:Vitamin K / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology*; D024482:Vitamin K 2 / Q000031:analogs & derivatives
D000700:Analgesics; D024482:Vitamin K 2; D014812:Vitamin K; C030814:menatetrenone; D013373:Substance P; D016202:N-Methylaspartate; D015232:Dinoprostone; D001920:Bradykinin