Glycolipid antigen processing for presentation by CD1d molecules.
The requirement for processing glycolipid antigens in T cell recognition was examined with mouse CD1d-mediated responses to glycosphingolipids (GSLs). Although some disaccharide GSL antigens can be recognized without processing, the responses to three other antigens, including the disaccharide GSL Gal(alpha1-->2)GalCer (Gal, galactose; GalCer, galactosylceramide), required removal of the terminal sugars to permit interaction with the T cell receptor. A lysosomal enzyme, alpha-galactosidase A, was responsible for the processing of Gal(alpha1-->2)GalCer to generate the antigenic monosaccharide epitope. These data demonstrate a carbohydrate antigen processing system analogous to that used for peptides and an ability of T cells to recognize processed fragments of complex glycolipids.
Prigozy
T I
TI
Division of Developmental Immunology, La Jolla Institute for Allergy and Immunology, 10355 Science Center Drive, San Diego, CA 92121, USA.
Naidenko
O
O
Qasba
P
P
Elewaut
D
D
Brossay
L
L
Khurana
A
A
Natori
T
T
Koezuka
Y
Y
Kulkarni
A
A
Kronenberg
M
M
D020816:Amino Acid Motifs; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D017951:Antigen Presentation; D000938:Antigen-Presenting Cells / Q000276:immunology; D018949:Antigens, CD1 / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D055610:Antigens, CD1d; D002236:Carbohydrate Conformation; D016207:Cytokines / Q000096:biosynthesis; D000939:Epitopes / Q000276:immunology; D005699:Galactosylceramides / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism*; D008247:Lysosomes / Q000201:enzymology; D018942:Macrolides; D051379:Mice; D008810:Mice, Inbred C57BL; D021381:Protein Transport; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014162:Transfection; D014407:Tumor Cells, Cultured; D000519:alpha-Galactosidase / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D018949:Antigens, CD1; D055610:Antigens, CD1d; D016207:Cytokines; D000939:Epitopes; D005699:Galactosylceramides; D018942:Macrolides; D011948:Receptors, Antigen, T-Cell; C036978:concanamycin A; C040929:bafilomycin A1; D000519:alpha-Galactosidase