title: Archaeal promoter-directed expression of the Halobacterium salinarum catalase-peroxidase gene.
abstract: The Halobacterium salinarum catalase-peroxidase gene was subcloned into shuttle vectors pWL102 and pWL202 and expressed under the control of different archaeal promoters. When Hbt. salinarum was transformed with the catalase-peroxidase gene under the control of its own promoter, catalase-peroxidase activity increased twofold. Catalase-peroxidase activity increased threefold when Hbt. salinarum was transformed with the catalase-peroxidase gene under the control of a tRNA promoter. This bifunctional enzyme in Hbt. salinarum was not induced by environmental stresses such as H2O2, intense light, darkness, high temperature, low temperature, redox inhibitors, heavy metals, or ions.
authors.affiliation: Department of Biochemistry and Molecular Biology, Mississippi State University, 39762, USA. mls1@ra.msstate.edu
authors.forename: S
authors.initials: S
authors.lastname: Long
authors.forename: M L
authors.initials: ML
authors.lastname: Salin
mesh_terms: D001483:Base Sequence; D002374:Catalase / Q000096:biosynthesis / Q000235:genetics*; D017931:DNA Primers; D004790:Enzyme Induction; D019848:Gene Expression Regulation, Archaeal / Q000235:genetics*; D006217:Halobacterium / Q000201:enzymology / Q000235:genetics*; D018384:Oxidative Stress; D010544:Peroxidases / Q000096:biosynthesis / Q000235:genetics*; D011401:Promoter Regions, Genetic
chemical_list: D017931:DNA Primers; D010544:Peroxidases; D002374:Catalase