Abnormal Ca2+ handling and increased Mg2+ permeability in platelets of hypertensive rats.
In order to test the hypothesis that intracellular Na+ accumulation and cellular Mg2+ deficiency may be involved in the abnormalities in Ca2+ handling and reactivity in spontaneously hypertensive rats (SHR) platelets, the metabolism of Na+, Ca2+ and Mg2+ was determined in fluorescent dye loaded platelets from 15 SHR and 15 Wistar-Kyoto rats (WKY) at 12 weeks of age. Mg2+ leak was estimated as the Mg2+ influx induced by an increase in extracellular [Mg2+] (from 1 to 5 mmol/l) and Mg2+/Na+ exchange activity was estimated as the Mg2+ influx induced by a decrease in extracellular [Na+] (from 140 to 50 mmol/l). Cellular metabolism of the fluorescent dye was similar in the two groups. Mean platelet [Ca2+]i was significantly increased under basal and thrombin (0.1 U/ml)-stimulated conditions in SHR compared to WKY, both in the presence and absence of extracellular Ca2+. Mean Ca2+ discharge capacity was similar between the two groups. There was no difference in mean [Na+]i between the two groups. Basal [Mg2+]i was also increased in SHR platelets. Mg2+ leak was higher in SHR than in WKY, while Mg2+/Na+ exchange activity was similar in the two groups. There was no difference in serum Mg2+ concentration between SHR and WKY. These data suggest that abnormal Ca2+ handling is accompanied by elevation in [Mg2+]i via increased permeability of platelet cell membranes to Mg2+ in SHR without any alteration in [Na+]i, and do not support the Mg2+ deficiency hypothesis in genetically hypertensive rats.
Oshima
T
T
Department of Clinical Laboratory Medicine, Hiroshima University School of Medicine, Japan.
Nakano
Y
Y
Ozono
R
R
Higashi
Y
Y
Sasaki
S
S
Ueda
A
A
Oue
Y
Y
Matsuura
H
H
Kambe
M
M
D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D001794:Blood Pressure; D002118:Calcium / Q000097:blood*; D002463:Cell Membrane Permeability; D006339:Heart Rate; D006973:Hypertension / Q000097:blood* / Q000503:physiopathology; D007425:Intracellular Membranes / Q000378:metabolism; D008274:Magnesium / Q000097:blood*; D009994:Osmolar Concentration; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D012964:Sodium / Q000097:blood
D012964:Sodium; D008274:Magnesium; D002118:Calcium