How central is nitric oxide (NO) to the activation of c-fos in spinal neurones following noxious peripheral stimulation in the rat?
Intrathecal application of high doses of NO-donor compounds in the anaesthetised rat was not found to cause any induction of c-fos in spinal neurones. Furthermore, intrathecal injection of a NO-synthase (NOS) blocking drug did not alter the numbers of c-fos positive neurones induced by noxious stimulation. Additionally very little colocalization between NOS and c-fos following noxious stimulation was found. Collectively these data give no support for a role for NO in the noxiously evoked induction of c-fos.
Nazli
M
M
Department of Veterinary Anatomy, University of Kafkas, Kars, Turkey.
Hismiogullari
E S
ES
Thippeswamy
T
T
Morris
R
R
D000818:Animals; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D008297:Male; D009149:Mustard Plant; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009474:Neurons / Q000201:enzymology*; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D009619:Nociceptors / Q000378:metabolism*; D009676:Noxae; D010146:Pain / Q000139:chemically induced / Q000378:metabolism; D010525:Peripheral Nerves / Q000502:physiology; D010936:Plant Extracts; D010938:Plant Oils; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013116:Spinal Cord / Q000166:cytology*
D004791:Enzyme Inhibitors; D009676:Noxae; D010936:Plant Extracts; D010938:Plant Oils; D016760:Proto-Oncogene Proteins c-fos; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; C027793:mustard oil; D019331:NG-Nitroarginine Methyl Ester