title: A single amino acid substitution in the ICP27 protein of herpes simplex virus type 1 is responsible for its resistance to leptomycin B.
abstract: Leptomycin B (LMB) is a specific inhibitor of Crm1-dependent nuclear export of proteins. The replication of herpes simplex virus (HSV) is normally highly sensitive to LMB; a resistant HSV variant, however, was isolated by serial passages of the virus. Analysis of marker transfer and viral DNA sequences revealed that a single amino acid substitution within the ICP27 gene is responsible for conferring this resistance.
authors.affiliation: Laboratory of Virology, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Showa-ku, Nagoya 466-8550, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Murata
authors.forename: F
authors.initials: F
authors.lastname: Goshima
authors.forename: T
authors.initials: T
authors.lastname: Koshizuka
authors.forename: H
authors.initials: H
authors.lastname: Takakuwa
authors.forename: Y
authors.initials: Y
authors.lastname: Nishiyama
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D002522:Chlorocebus aethiops; D004352:Drug Resistance, Microbial; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D018259:Herpesvirus 1, Human / Q000187:drug effects* / Q000235:genetics / Q000502:physiology*; D006801:Humans; D017874:Immediate-Early Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014709:Vero Cells; D014779:Virus Replication / Q000187:drug effects
chemical_list: D005231:Fatty Acids, Unsaturated; C057120:ICP27 protein, human herpesvirus 1; D017874:Immediate-Early Proteins; C038753:leptomycin B