title: [Use of electrophoresis in polyacrylomide gel (SDS_PAGE) for evaluating IgG structure--the main component of human intravenous immunoglobulin].
abstract: For the assessment of the structure of IgG particles in the immunoglobulin preparation for intravenous use (Bioglobulin) electrophoresis in polyacrylamide gel (SDS-PAGE) was applied in place of filtration on Sephadex G-200 gel. The method serves for determination of the molecular weight of polypeptide chains under conditions of reduction. The method is rapid, sensitive and of high resolution. Six batches of the preparation were examined finding that IgG monomers and dimers accounted for over 90%, with IgG aggregates about 3% and degradation products about 1%. The obtained values comply with the recommendation of the WHO and requirements in the latest edition of Europea Pharmacopaes.
authors.affiliation: Zakład Badania Surowic i Szczepionek PZH w Warszawie.
authors.forename: M
authors.initials: M
authors.lastname: Kwas
authors.forename: B
authors.initials: B
authors.lastname: Bucholc
authors.forename: J
authors.initials: J
authors.lastname: Slusarczyk
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis* / Q000737:chemistry; D016756:Immunoglobulins, Intravenous / Q000032:analysis* / Q000592:standards; D008970:Molecular Weight
chemical_list: D007074:Immunoglobulin G; D016756:Immunoglobulins, Intravenous