Reciprocal regulatory interaction between human herpesvirus 8 and human immunodeficiency virus type 1.
Human herpesvirus 8 (HHV8) is the primary viral etiologic agent in Kaposi's sarcoma (KS). However, individuals dually infected with both HHV8 and human immunodeficiency virus type 1 (HIV-1) show an enhanced prevalence of KS when compared with those singularly infected with HHV8. Host immune suppression conferred by HIV infection cannot wholly explain this increased presentation of KS. To better understand how HHV8 and HIV-1 might interact directly in the pathogenesis of KS, we queried for potential regulatory interactions between the two viruses. Here, we report that HHV8 and HIV-1 reciprocally up-regulate the gene expression of each other. We found that the KIE2 immediate-early gene product of HHV8 interacted synergistically with Tat in activating expression from the HIV-1 long terminal repeat. On the other hand, HIV-1 encoded Tat and Vpr proteins increased intracellular HHV8-specific expression. These results provide molecular insights correlating coinfection with HHV8 and HIV-1 with an unusually high incidence of KS.
Huang
L M
LM
Department of Pediatrics, National Taiwan University Hospital, National Health Research Institutes, Taipei 100, Taiwan. lmhuang@ha.mc.ntu.edu.tw
Chao
M F
MF
Chen
M Y
MY
Shih Hm
Chiang
Y P
YP
Chuang
C Y
CY
Lee
C Y
CY
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications / Q000503:physiopathology; D002459:Cell Fusion; D002460:Cell Line; D015696:Gene Products, tat / Q000378:metabolism; D016325:HIV Long Terminal Repeat; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006367:HeLa Cells; D019288:Herpesvirus 8, Human / Q000235:genetics / Q000502:physiology*; D006706:Homeostasis; D006801:Humans; D019169:Jurkat Cells; D011401:Promoter Regions, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012260:Ribonucleases / Q000378:metabolism; D012514:Sarcoma, Kaposi / Q000503:physiopathology* / Q000821:virology; D014162:Transfection; D054322:tat Gene Products, Human Immunodeficiency Virus
D015696:Gene Products, tat; D054322:tat Gene Products, Human Immunodeficiency Virus; D012260:Ribonucleases