[Cytokine production by different population of macrophages following radiation or combined radiation injury].
Male mice (CBA x C57BL6)F1 were used for the experiments throughout this study. The levels of spontaneous and LPS-stimulated cytokines production (IL-1 beta, IL-6 and TNF-alpha) by peritoneal, splenic, and bone marrow macrophages were evaluated by means of enzyme-linked immunosorbent assay at 3, 6, 24, 48 and 72 hours after irradiation alone or combined injury (irradiation + thermal burn). The results suggest that macrophages, harvested from the main mice hematopoietic organs (bone marrow, spleen), did not increase cytokines production within the first three days following the 7 Gy gamma-irradiation or combined injury. Peritoneal macrophages revealed a capacity to enhance IL-6 and IL-1 production versus normal healthy mice. There were no significant differences of cytokine-producing activity if macrophages were harvested from irradiated or combined injured mice.
Budagov
R S
RS
Medical Radiological Research Center, Russian Academy of Medical Sciences, Obninsk, 249020 Russia. Budagov@obninsk.com
Ul'ianova
L P
LP
D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D002056:Burns / Q000378:metabolism* / Q000473:pathology; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D007375:Interleukin-1 / Q000096:biosynthesis*; D015850:Interleukin-6 / Q000096:biosynthesis*; D008264:Macrophages / Q000378:metabolism* / Q000473:pathology / Q000528:radiation effects; D017737:Macrophages, Peritoneal / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011833:Radiation Injuries, Experimental / Q000378:metabolism* / Q000473:pathology; D013154:Spleen / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D007375:Interleukin-1; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha