Differential regulation of the secretions of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinases-1 from human keratinocytes in culture.
To investigate the pathophysiological roles of matrix metalloproteinase (MMP)-9 and tissue inhibitor of metalloproteinases (TIMP)-1 in the differentiation of keratinocytes, we used human keratinocytes in culture to examine the gelatinolytic activity of MMP-2 and MMP-9 by zymographic analysis, MMP-9 mRNA by reverse transcriptase-polymerase chain reaction, and TIMP-1 mRNA by Northern blot analysis. Adding of calcium (1 mM) to the culture medium increased the activity of MMP-9 and MMP-9 mRNA without affecting MMP-2 activity or the expression of TIMP-1 mRNA. These results suggest that keratinocytes regulate the expressions of MMP-9 and TIMP-1 differently during the process of differentiation.
Kobayashi
T
T
Department of Dermatology, Chiba University School of Medicine, Japan. tkobayas@derma01.m.chiba-u.ac.jp
Hattori
S
S
Nagai
Y
Y
Tajima
S
S
D001483:Base Sequence; D002454:Cell Differentiation; D002478:Cells, Cultured; D017931:DNA Primers / Q000235:genetics; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics / Q000378:metabolism*
D017931:DNA Primers; D012333:RNA, Messenger; D019715:Tissue Inhibitor of Metalloproteinase-1; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9