Comparative sequence analyses of the ribonuclease P (RNase P) RNA genes from LL-2,6-diaminopimelic acid-containing actinomycetes.
Ribonuclease P (RNase P) RNA genes of LL-2,6-diaminopimelic acid (LL-A2pm)-containing actinomycetes, except Streptomyces species, were sequenced after PCR-amplification and cloning. By using the sequence data, the relationships between species within genera and the relationships between taxa above genus level were investigated and the usefulness of the RNase P RNA gene as another phylogenetic marker was evaluated. RNase P RNA gene sequences of all strains used in this study contained relatively conserved regions along with highly variable regions. The mean RNase P RNA gene similarity value was approximately 82 +/- 18% and the mean RNase P RNA gene similarity value when gaps were included was approximately 76 +/- 24%. The nucleotide similarities between the RNase P RNA genes of different strains were mostly fewer than the 16S rDNA similarities. The RNase P RNA gene was more useful than 16S rDNA for clearly differentiating the relationships between species belonging to a genus and the relationships between some genera. However, nucleotide sequences of RNase P RNA genes were not necessarily appropriate for comparisons at all taxonomic levels (such as those between species, between genera and between families).
Yoon
J H
JH
Park
Y H
YH
D000192:Actinomycetales / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000254:growth & development; D003001:Cloning, Molecular; D004275:DNA, Ribosomal / Q000032:analysis; D003960:Diaminopimelic Acid / Q000378:metabolism; D004722:Endoribonucleases / Q000235:genetics* / Q000378:metabolism; D020459:Genes, rRNA / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016337:RNA, Catalytic / Q000235:genetics* / Q000378:metabolism; D012335:RNA, Ribosomal / Q000235:genetics; D043262:Ribonuclease P; D017422:Sequence Analysis, DNA
D004275:DNA, Ribosomal; D016337:RNA, Catalytic; D012335:RNA, Ribosomal; D003960:Diaminopimelic Acid; D004722:Endoribonucleases; D043262:Ribonuclease P