Detection of lymphotropic herpesvirus DNA by polymerase chain reaction in cerebrospinal fluid of AIDS patients with neurological disease.
Cerebrospinal fluid (CSF) samples from 49 acquired immunodefficiency disease syndrome (AIDS) patients with a central nervous system (CNS) disease were examined by polymerase chain reaction (PCR) to evaluate the association between the positivity for cytomegalovirus (CMV) and Epstein-Barr virus (EBV), and clinical diagnosis of a CNS disease. Frequency and clinical relevance of detection of DNA of human herpesviruses 6 (HHV-6), 7 (HHV-7) and 8 (HHV-8) were also determined. DNA of one or more of the following viruses was found in 26 of 49 patients (53%): CMV in 16 (33%), EBV in 13 (27%), human herpesvirus 6 (HHV-6) in 2 (4%), human herpesvirus 7 (HHV-7) in 1 (2%), and human herpesvirus 8 (HHV-8) in 1 (2%). The CMV detection was significantly associated with encephalitis and peripheral neuropathy (7/16 vs. 2/33, p = 0.003), while EBV with primary CNS lymphoma (P-CNSL) (8/13 vs. 0/36, p < 0.0001). HHV-6 DNA was found in CSF of two patients with neuroradiological features suggestive of cerebral lesions. HHV-8 or HHV-7 DNA was detected in the CSF of patients with unexplained neurological symptoms. This study confirms that the PCR analysis of CSF is a valid tool for the diagnosis of neurological diseases associated with CMV and EBV. On the other hand, HHV-6, HHV-7 and HHV-8, instead, were rarely detected in CSF of AIDS patients and have certainly no correlation with the CNS disease found.
Broccolo
F
F
Department of Experimental Pathology, Medical Biotechnology, Infectious Diseases and Epidemiology, University of Pisa, 56127 Pisa, Italy. broccolo.francesco@hsr.it
Iuliano
R
R
Careddu
A M
AM
Trovato
R
R
Lico
S
S
Blanc
P L
PL
Mazzotta
F
F
Ceccherini-Nelli
L
L
D017088:AIDS-Related Opportunistic Infections / Q000134:cerebrospinal fluid / Q000821:virology*; D000163:Acquired Immunodeficiency Syndrome / Q000150:complications*; D002493:Central Nervous System Diseases / Q000134:cerebrospinal fluid / Q000150:complications* / Q000821:virology; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000134:cerebrospinal fluid / Q000150:complications* / Q000821:virology; D004279:DNA, Viral / Q000134:cerebrospinal fluid*; D018792:Encephalitis, Viral / Q000150:complications / Q000821:virology; D020031:Epstein-Barr Virus Infections / Q000134:cerebrospinal fluid / Q000150:complications / Q000821:virology; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification; D015654:Herpesvirus 6, Human / Q000235:genetics / Q000302:isolation & purification; D016199:Herpesvirus 7, Human / Q000235:genetics / Q000302:isolation & purification; D019288:Herpesvirus 8, Human / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D016483:Lymphoma, AIDS-Related / Q000150:complications / Q000821:virology; D010523:Peripheral Nervous System Diseases / Q000150:complications / Q000821:virology; D016133:Polymerase Chain Reaction
D004279:DNA, Viral