Dendritic cells are sufficient to cross-present self-antigens to CD8 T cells in vivo.
The mechanism of cross-presentation enables professional APCs to induce CD8 T cell-mediated immune responses against exogenous Ags. Through this mechanism, APCs can induce either immunity against infectious pathogens or tolerance against self-Ag residing in extralymphatic locations. An unanswered question in this field concerns the identity of the cross-presenting APC. All major classes of professional APCs, particularly dendritic cells, macrophages, and B cells, have previously been shown to be able to cross-present Ags in vitro. In the present study, we have created transgenic mice where MHC class I expression is driven selectively in dendritic cells and provide direct in vivo evidence that dendritic cells are sufficient to cross-present exogenous self-Ags and induce Ag-specific cell division of CD8-positive T cells.
Kurts
C
C
Department of Nephrology and Immunology, University of Aachen, Aachen, Germany.
Cannarile
M
M
Klebba
I
I
Brocker
T
T
D019264:Adoptive Transfer; D000818:Animals; D017951:Antigen Presentation / Q000235:genetics; D001324:Autoantigens / Q000276:immunology* / Q000378:metabolism*; D016026:Bone Marrow Transplantation / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000637:transplantation*; D002460:Cell Line; D003433:Crosses, Genetic; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis / Q000235:genetics; D007328:Insulin / Q000235:genetics; D016167:Integrin alphaXbeta2 / Q000096:biosynthesis / Q000235:genetics; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010047:Ovalbumin / Q000276:immunology / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000276:immunology; D051381:Rats; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D019076:Transgenes / Q000276:immunology; D001613:beta 2-Microglobulin / Q000096:biosynthesis / Q000235:genetics
D001324:Autoantigens; D015395:Histocompatibility Antigens Class I; D007328:Insulin; D016167:Integrin alphaXbeta2; D001613:beta 2-Microglobulin; D010047:Ovalbumin