Amplified fragment length polymorphism fingerprinting of 16 banana cultivars (Musa cvs.).
Banana is one of the most important subtropical crops. The genetic system, however, is relatively unknown and is complicated by specific interhybridization, heterozygosity, and polyploidy, which are common in most clones. These factors make identification of closely related banana cultivars difficult, particularly when sterile. Amplified fragment length polymorphism (AFLP) analysis using eight primer combinations was carried out on 16 banana cultivars. Results showed that AFLP could be used to distinguish the different cultivars by their unique banding patterns. Unique AFLP molecular markers were detected for 12 banana cultivars, which can be used to develop specific probes for identification purposes. The cluster analysis also revealed the need for a link between genotype studies using molecular techniques and the current system of classification of Musa cultivars based purely on morphological traits.
Loh
J P
JP
School of Science, National Institute of Education, Nanyang Technological University, 469 Bukit Timah Road, 259756, Singapore.
Kiew
R
R
Set
O
O
Gan
L H
LH
Gan
Y Y
YY
D016172:DNA Fingerprinting; D017931:DNA Primers; D005819:Genetic Markers / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics; D005838:Genotype; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D019862:Zingiberales / Q000145:classification* / Q000235:genetics*
D017931:DNA Primers; D005819:Genetic Markers