title: Flavonoids and phenolic acids of sage: influence of some agricultural factors.
abstract: A reversed-phase high-performance liquid chromatography/diode-array detector procedure is proposed for the determination of six phenolic compounds (caffeic acid, luteolin 7-O-glucoside, rosmarinic acid, apigenin, hispidulin, and cirsimaritin) in sage. The chromatographic separation was achieved using a reversed-phase Spherisorb ODS2 (5-microm particle size, 25.0 x 0.46 cm) column. Of the several extractive solvents assayed only ethyl ether, ethyl acetate, and acetone were able to extract all the compounds mentioned. Best resolution was obtained using a gradient of water/phosphoric acid (999:1) and acetonitrile. Ten samples cultivated in two experimental fields (1997-1999) were analyzed and the individual compounds quantified. Four commercially available samples were also analyzed and the results are discussed.
authors.affiliation: CEQUP/Laboratorio de Farmacognosia, Faculdade de Farmacia, Universidade do Porto, R. Anibal Cunha, 4050-047 Porto, Portugal.
authors.forename: F
authors.initials: F
authors.lastname: Areias
authors.forename: P
authors.initials: P
authors.lastname: Valentão
authors.forename: P B
authors.initials: PB
authors.lastname: Andrade
authors.forename: F
authors.initials: F
authors.lastname: Ferreres
authors.forename: R M
authors.initials: RM
authors.lastname: Seabra
mesh_terms: D005419:Flavonoids / Q000032:analysis*; D062385:Hydroxybenzoates / Q000032:analysis*; D019686:Lamiaceae / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D005419:Flavonoids; D062385:Hydroxybenzoates; C017616:phenolic acid