[The relationship between the change of mitochondria and the fertility in CMS line of sorghum after heat shock].
The mitochondria of anther in pollen mother cell stage of cytoplasmic male sterility (CMS) line 3197A in sorghum were extracted after heat shock. The number of the mitochondria was measured by flow cytometer and some isoenzymes were analyzed by PAGE combined with stain for enzyme activity. The result of mitochondria quantity measurement shows that the number of the mitochondria in CMS line 3197A increased greatly up to 4.5 folds after heat shock. The analysis of isoenzymes also demonstrates that the type of isoenzyme in CMS line is in consistent with maintainer line. The study denoted the change of the quantity of mitochondria after heat shock and the relationship between this change and the fertility in sorghum.
Wang
L
L
The Institute of Genetics, Chinese Academy of Sciences, 100101, China.
Qi
X H
XH
Liu
G Q
GQ
Zhang
Y M
YM
Chen
J N
JN
D002523:Edible Grain / Q000235:genetics* / Q000502:physiology; D006358:Hot Temperature; D007527:Isoenzymes / Q000378:metabolism; D008928:Mitochondria / Q000502:physiology*; D010935:Plant Diseases / Q000209:etiology*
D007527:Isoenzymes