Microarray analysis of diurnal and circadian-regulated genes in Arabidopsis.
Plants respond to day/night cycling in a number of physiological ways. At the mRNA level, the expression of some genes changes during the 24-hr period. To identify novel genes regulated in this way, we used microarrays containing 11,521 Arabidopsis expressed sequence tags, representing an estimated 7800 unique genes, to determine gene expression levels at 6-hr intervals throughout the day. Eleven percent of the genes, encompassing genes expressed at both high and low levels, showed a diurnal expression pattern. Approximately 2% cycled with a circadian rhythm. By clustering microarray data from 47 additional nonrelated experiments, we identified groups of genes regulated only by the circadian clock. These groups contained the already characterized clock-associated genes LHY, CCA1, and GI, suggesting that other key circadian clock genes might be found within these clusters.
Schaffer
R
R
Michigan State University-Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824-1312, USA.
Landgraf
J
J
Accerbi
M
M
Simon
V
V
Larson
M
M
Wisman
E
E
D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology; D001483:Base Sequence; D002940:Circadian Rhythm; D017931:DNA Primers; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D017343:Genes, Plant; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction
D017931:DNA Primers