Comparative prevalences of Brachyspira aalborgi and Brachyspira (Serpulina) pilosicoli as etiologic agents of histologically identified intestinal spirochetosis in Australia.
DNA from gastrointestinal biopsy specimens from 28 Australian patients with histologic evidence of intestinal spirochetosis (IS) was subjected to PCRs to amplify segments of the 16S rRNA and NADH oxidase genes of Brachyspira aalborgi and Brachyspira (Serpulina) pilosicoli. B. aalborgi was identified in specimens from 24 (85.7%) patients and B. pilosicoli in those from 4 (14.3%) patients (2 of whom were also positive for B. aalborgi). For two patients, no product was amplified. This study demonstrates that B. aalborgi is much more commonly involved in histologically identified IS in Australian patients than is B. pilosicoli. This is the first report of amplification of B. pilosicoli DNA from humans with IS.
Mikosza
A S
AS
Division of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia.
La
T
T
de Boer
W B
WB
Hampson
D J
DJ
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001315:Australia / Q000453:epidemiology; D017400:Brachyspira / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D005260:Female; D006801:Humans; D007410:Intestinal Diseases / Q000453:epidemiology* / Q000382:microbiology*; D007422:Intestines / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D009097:Multienzyme Complexes / Q000235:genetics; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013144:Spirochaetales / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D013145:Spirochaetales Infections / Q000453:epidemiology* / Q000382:microbiology
D004269:DNA, Bacterial; D009097:Multienzyme Complexes; D012336:RNA, Ribosomal, 16S; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases