Degradation of gluten by proteases from dry and germinating wheat (Triticum durum) seeds: An in vitro approach to storage protein mobilization.
Vital gluten was used as an ideal substrate to investigate the role of some proteases in storage protein degradation. Aspartic proteinase and carboxypeptidase were identified as endogenous enzymes adsorbed on gluten and their optimum pH values determined. SDS-PAGE of soluble products released by gluten digestion revealed that the activity of these proteases plays a minor role in protein mobilization, whereas cysteine proteinase, purified from wheat seeds at the fourth day of germination, is extremely effective, producing a remarkable protein degradation in short times. Synergistic effects of aspartic and cysteine proteinase were not observed. Spin labeling of the sulfhydryl groups of gluten proteins enabled a comparative EPR investigation of the consequences of proteolytic degradation on gluten elasticity. It was found that storage protein mobilization brings a loss of elasticity to the polymeric network of gluten, which is particularly marked when the hydrolysis is performed by cysteine proteinase.
Capocchi
A
A
Dipartimento di Scienze Botaniche, Universita di Pisa, Italy.
Cinollo
M
M
Galleschi
L
L
Saviozzi
F
F
Calucci
L
L
Pinzino
C
C
Zandomeneghi
M
M
D010450:Endopeptidases / Q000378:metabolism*; D005983:Glutens / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010940:Plant Proteins / Q000378:metabolism*; D012639:Seeds / Q000201:enzymology*; D014908:Triticum
D010940:Plant Proteins; C038234:storage protein, Triticum aestivum; D005983:Glutens; D010450:Endopeptidases