Characterization of the common nodulation genes of the photosynthetic Bradyrhizobium sp. ORS285 reveals the presence of a new insertion sequence upstream of nodA.
We isolated and characterized nodA genes from photosynthetic and non-photosynthetic rhizobia nodulating the legume genus Aeschynomene, and found that the nodA sequence from photosynthetic stem-nodulating bacteria was phylogenetically distant from the other already described nodA genes. Characterization of the photosynthetic strain ORS285 common nod gene cluster (nodABC) showed, upstream of nodA, the presence of a new insertion sequence element belonging to the IS3 family and specific to a group of photosynthetic strains from Aeschynomene.
Chaintreuil
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C
Laboratoire des Symbioses Tropicales et Méditerranéennes, IRD, CIRAD, AGRO-M, INRA TA 10/J, Campus de Baillarguet, 34398 Cedex 5, Montpellier, France.
Boivin
C
C
Dreyfus
B
B
Giraud
E
E
D000217:Acyltransferases / Q000235:genetics*; D001426:Bacterial Proteins; D020369:Bradyrhizobium / Q000235:genetics* / Q000502:physiology; D004251:DNA Transposable Elements; D007887:Fabaceae / Q000382:microbiology; D005798:Genes, Bacterial; D015698:Genomic Library; D008969:Molecular Sequence Data; D005810:Multigene Family; D009586:Nitrogen Fixation / Q000235:genetics*; D010788:Photosynthesis / Q000502:physiology*; D010802:Phylogeny; D010946:Plants, Medicinal; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D000217:Acyltransferases; C060770:NodA protein, Rhizobiales