The effect of fosfomycin on neutrophil function.
Fosfomycin (cis-1,2-epoxypropyl phosphonic acid) is a cell wall synthesis-inhibiting antibiotic. We investigated the effect of fosfomycin on several indices of neutrophil function. Neutrophil phagocytosis was analysed by flow cytometry. Cytosolic calcium kinetics were assessed fluorometrically and neutrophil bactericidal ability was assessed by fluorescence microscopy. Intracellular reactive oxygen intermediate (ROI) production was analysed by flow cytometry and extracellular ROI by cytochrome c reductase assay. After fosfomycin incubation, phagocytosis was unaffected as assessed by the FACS assay. Fosfomycin incubation resulted in enhanced bactericidal ability, in increased intracellular calcium concentrations, elevated extracellular ROI production and decreased chemotaxis but it did not affect intracellular ROI production and chemokinesis.
Krause
R
R
Division of Infectious Diseases, Department of Medicine, Karl-Franzens University Graz, Auenbruggerplatz 15, A-8036 Graz, Austria. robert.krause@kfunigraz.ac.at
Patruta
S
S
Daxböck
F
F
Fladerer
P
P
Wenisch
C
C
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002469:Cell Separation; D018925:Chemokines / Q000378:metabolism; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D005434:Flow Cytometry; D005578:Fosfomycin / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D009245:NADH Dehydrogenase / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism
D000900:Anti-Bacterial Agents; D018925:Chemokines; D017382:Reactive Oxygen Species; D005578:Fosfomycin; D009245:NADH Dehydrogenase; D002118:Calcium