Differential expression of manganese peroxidase and laccase in white-rot fungi in the presence of manganese or aromatic compounds.
White-rot fungi (basidiomycetes) play an important role in the degradation of lignin which is, beside cellulose, the major compound of wood. This process is catalyzed by ligninolytic enzymes, which are able to cleave oxidatively aromatic rings in lignin structure. Manganese peroxidase and laccase of white-rot-fungi are the most important of these among the ligninolytic enzymes. In addition, they are able to degrade xenobiotic aromatic polymers, persisting as environmental pollutants. Manganese and aromatic compounds have often been discussed as being inducers, enhancers or mediators of these ligninolytic enzymes. It is known that supplementing the growth medium with either Mn2+, veratryl alcohol or coal-derived humic acids leads to significantly enhanced extracellular ligninolytic activities. Measuring the amount of expressed mRNA of the two enzymes by quantitative RT-PCR provided evidence that the expression of manganese peroxidase was induced in the three tested white-rot fungi, Clitocybula dusenii b11, Nematoloma frowardii b19, and a straw-degrading strain designated i63-2. Laccase, on the other hand, was expressed in all three fungi with a significant basic activity even without inducer added. However, since the level of laccase mRNA was higher in cultures supplemented with any one of the tested inducers, we conclude that both manganese and the aromatic substances also increase the expression of laccase.
Scheel
T
T
Botanisches Institut der Universität Bonn, Germany.
Höfer
M
M
Ludwig
S
S
Hölker
U
U
D001487:Basidiomycota / Q000201:enzymology* / Q000235:genetics; D001592:Benzyl Alcohols / Q000494:pharmacology*; D003470:Culture Media; D018076:DNA, Complementary; D004790:Enzyme Induction; D015971:Gene Expression Regulation, Enzymologic; D005800:Genes, Fungal; D006812:Humic Substances / Q000494:pharmacology*; D042845:Laccase; D008345:Manganese / Q000378:metabolism / Q000494:pharmacology*; D010088:Oxidoreductases / Q000096:biosynthesis* / Q000235:genetics; D010544:Peroxidases / Q000096:biosynthesis* / Q000235:genetics; D016133:Polymerase Chain Reaction; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D001592:Benzyl Alcohols; D003470:Culture Media; D018076:DNA, Complementary; D006812:Humic Substances; D012331:RNA, Fungal; D012333:RNA, Messenger; D008345:Manganese; D010088:Oxidoreductases; D042845:Laccase; D010544:Peroxidases; C051129:manganese peroxidase; C042197:veratryl alcohol