Identification of endogenous gibberellins in strawberry, including the novel gibberellins GA123, GA124 and GA125.
Extracts of carboxylic acids from immature fruits of strawberry (Fragaria x ananassa Duch. cv. Elsanta) were analysed for gibberellins by combined gas chromatography-mass spectrometry. The following previously characterised gibberellins were identified by comparison of their mass spectra and Kovats retention indices (KRIs) with those of standards or published data: GA1, GA3, GA5, GA8, GA12, GA17, GA19, GA20, GA29, GA44, GA48, GA49, GA53, GA77, GA97, GA111 and GA112. Evidence for endogenous 1-epi GA61 (GA119) and 11alpha-OH-GA12 was also obtained. In addition, a number of putative GAs were detected. Of these, three were shown to be 12alpha-hydroxy-GA53, 12alpha-hydroxy-GA44, and 12alpha-hydroxy-GA19 by comparison with authentic compounds prepared by rational synthesis, and have been allocated the descriptors GA123, GA124 and GA125, respectively.
Blake
P S
PS
Horticulture Research International, West Malling, Kent, UK. patrick.blake@hri.ac.uk
Taylor
D R
DR
Crisp
C M
CM
Mander
L N
LN
Owen
D J
DJ
D005638:Fruit / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D005875:Gibberellins / Q000737:chemistry / Q000302:isolation & purification*; D008968:Molecular Conformation; D019685:Rosales / Q000737:chemistry*
D005875:Gibberellins