Study of phospholipases D and C in maturing and germinating seeds of Brassica napus.
Different forms of phospholipase D (dependent on and independent of the presence of phosphatidylinositol 4,5-bisphosphate, PIP(2)) were identified in maturing and germinating seeds of Brassica napus. Both forms were present in cytosolic and membrane fractions of maturing seeds. PIP(2)-dependent activity increased continuously during seed germination, while PIP(2)-independent activity appeared mostly at the very beginning of seed maturation. PIP(2)-dependent activity was detected mainly in the plasma-membrane fraction. Phosphatidylinositol-specific phospholipase C (PI-PLC) was found only in membrane fractions of both types of developing rape seed tissues. The increasing activities of PLC and PIP(2)-dependent PLD were mainly detected in hypocotyls of seedlings. Some biochemical characteristics of both described enzymes are also presented.
Novotna
Z
Z
Institute of Chemical Technology Prague, Technicka 3, 166 28 Prague 6, Czech Republic.
Valentova
O
O
Martinec
J
J
Feltl
T
T
Nokhrina
K
K
D001937:Brassica / Q000201:enzymology / Q000254:growth & development / Q000502:physiology*; D003600:Cytosol / Q000201:enzymology; D007425:Intracellular Membranes / Q000201:enzymology; D007527:Isoenzymes / Q000378:metabolism; D007700:Kinetics; D008861:Microsomes / Q000201:enzymology; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000378:metabolism; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D054801:Phosphoinositide Phospholipase C; D010739:Phospholipase D / Q000378:metabolism*; D012639:Seeds / Q000201:enzymology; D010738:Type C Phospholipases / Q000378:metabolism*
D007527:Isoenzymes; D019269:Phosphatidylinositol 4,5-Diphosphate; D010738:Type C Phospholipases; D054801:Phosphoinositide Phospholipase C; D010739:Phospholipase D; D043265:Phosphatidylinositol Diacylglycerol-Lyase