title: Ischaemia/reperfusion contributes to colonic injury following experimental aortic surgery.
abstract: OBJECTIVE
  ischaemia of the colon is an important complication of abdominal aortic aneurysm (AAA) repair. The aim of this animal study was to investigate the effect of sequential ischaemia and reperfusion on sigmoid mucosal pO2 and its association with local ET-1 release.
  
  
  METHODS
  twelve pigs underwent colonic ischaemia followed by complete reperfusion. Six other animals were sham controls. A Clark-type microcatheter was used for continuous mucosal pO2 measurements. Serial systemic and inferior mesenteric vein blood samples were obtained for determination of ET-1 concentration. Neutrophil extravasation was assessed by tissue myeloperoxidase (MPO) activity.
  
  
  RESULTS
  arterial occlusion was associated with a gradual decrease of mucosal pO2 and local release of ET-1. After restoration of blood flow, mucosal pO2 returned to near baseline values, whereas ET-1 reached its maximum concentration during the reperfusion period. MPO activity was significantly increased.
  
  
  CONCLUSIONS
  colonic ischaemia and reperfusion causes neutrophil extravasation and local ET-1.
authors.affiliation: Department of Cardiovascular Surgery, Inselspital, University of Bern, Switzerland.
authors.forename: P U
authors.initials: PU
authors.lastname: Reber
authors.forename: M
authors.initials: M
authors.lastname: Peter
authors.forename: A G
authors.initials: AG
authors.lastname: Patel
authors.forename: E
authors.initials: E
authors.lastname: Stauffer
authors.forename: G
authors.initials: G
authors.lastname: Printzen
authors.forename: D
authors.initials: D
authors.lastname: Mettler
authors.forename: H
authors.initials: H
authors.lastname: Hakki
authors.forename: H W
authors.initials: HW
authors.lastname: Kniemeyer
mesh_terms: D000818:Animals; D017544:Aortic Aneurysm, Abdominal / Q000473:pathology / Q000601:surgery*; D003106:Colon / Q000098:blood supply* / Q000473:pathology; D019332:Endothelin-1 / Q000097:blood; D005260:Female; D007413:Intestinal Mucosa / Q000098:blood supply / Q000473:pathology; D007511:Ischemia / Q000473:pathology*; D008297:Male; D010101:Oxygen Consumption / Q000502:physiology; D015427:Reperfusion Injury / Q000473:pathology*; D013552:Swine
chemical_list: D019332:Endothelin-1