Neuroectodermal and endodermal expression of the ascidian Cdx gene is separated by metamorphosis.
Ascidians are a group of invertebrate chordates that exhibit a biphasic life history, with chordate-specific structures developing during embryogenesis (dorsal neural tube and notochord) and metamorphosis (pharyngeal gill slits and endostyle). Here we characterize the expression of a caudal/Cdx gene homologue, Hec-Cdx, from the ascidian Herdmania curvata. Vertebrate Cdx genes are expressed at gastrulation and in the posterior of the developing neural tube and endoderm. Hec-Cdx expression is initiated at the earliest stages of gastrulation, with peaks in RNA abundance occurring first during neurulation and tailbud extension and then in 3- to 5-day-old juveniles. Hec-Cdx is expressed in a pair of cells in the anterior lip of the blastopore in the late gastrula which form the most posterior portion of the neural plate. During tailbud formation expression is maintained in and solely restricted to these cells. During metamorphosis expression is localized to the intestine of the juvenile. These data, along with data for the H. curvata Otx gene, suggest that the evolution of the novel ascidian biphasic body plan was not accompanied by a deployment of these genes into new pathways but by a temporal separation of tissue-specific expression.
Hinman
V F
VF
Department of Zoology and Entomology, University of Queensland, Brisbane, Queensland 4072, Australia.
Becker
E
E
Degnan
B M
BM
D000595:Amino Acid Sequence; D000818:Animals; D019521:Body Patterning / Q000235:genetics; D000071616:CDX2 Transcription Factor; D017124:Conserved Sequence; D004064:Digestive System / Q000378:metabolism; D004475:Ectoderm / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D004707:Endoderm / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D005801:Genes, Homeobox; D018398:Homeodomain Proteins / Q000096:biosynthesis* / Q000235:genetics*; D008675:Metamorphosis, Biological / Q000235:genetics*; D008969:Molecular Sequence Data; D009420:Nervous System / Q000196:embryology* / Q000254:growth & development / Q000378:metabolism; D015534:Trans-Activators; D014561:Urochordata / Q000196:embryology / Q000235:genetics* / Q000254:growth & development
D000071616:CDX2 Transcription Factor; D018398:Homeodomain Proteins; D015534:Trans-Activators