[Research on improving rice resistance to the pest by B.t. and SBTi genes].
B.t. gene alone or and SBTi gene together were introduced into two elite indica rice varieties grown in South China by bombardment. And 21 independent transgenic lines containing B.t. gene and 4 independent transgenic lines containing B.t. gene and SBTi gene were obtained. Molecular and genetics analysis for R1 plants showed integration of multiple transgenes occurred at one genetic locus. Northern blot result proved B.t. gene expressed in R2 transgenic plants stably. Bioassays using R2 transgenic plants with leaf-folder (Cnaphalocrosis medinalis), indicated that transgenic rice plants are more resistant to the pest than untransformed control plants. And those transgenic plants containing B.t. and SBTi genes showed more resistance compared with those plants containing B.t. gene.
Wei
J W
JW
Biotechnology Research Center, Zhongshan University, Guangzhou 510275.
Xu
X P
XP
Chen
J T
JT
Zhang
L Y
LY
Fan
Y L
YL
Li
B J
BJ
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins; D004731:Endotoxins / Q000235:genetics*; D006460:Hemolysin Proteins; D012275:Oryza / Q000235:genetics*; D010572:Pest Control, Biological; D030821:Plants, Genetically Modified; D014170:Transformation, Genetic; D014361:Trypsin Inhibitors / Q000235:genetics*
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D014361:Trypsin Inhibitors; C030956:insecticidal crystal protein, Bacillus Thuringiensis