Values of markers of early and advanced glycation and lipoxidation in serum proteins of children with diabetes mellitus.
BACKGROUND
Advanced glycation endproducts (AGEs) have been established as one of the major factors responsible for the multi-organ damage seen in diabetes. AGEs and lipoxidation products, as e.g. MDA, and their adducts with proteins appear to be formed together in serum and tissues. A link between AGEs formation and increased lipoxidation at tissue damage is under investigation.


OBJECTIVE
The aim of the present study was to determine fructosamine (FAM), glycated haemoglobin (HbA1c) AGEs-specific fluorescence and MDA-protein adducts specific fluorescence in diabetic and in healthy children, with statistical evaluation of the relationship between the parameters assessed.


METHODS
Values of FAM and HbA1c (spectrophotometry) and of AGEs-specific fluorescence and MDA-protein adducts specific fluorescence were investigated in serum proteins of 17 children with poorly controlled type 1 diabetes mellitus (age range 9 to 18 years). Eight healthy children (age range 7 to 17 years) served as controls.


RESULTS
In the diabetic group, all the parameters evaluated were significantly higher than in the control group. Furthermore, MDA-linked specific fluorescence of MDA-protein adducts (a biomarker of oxidative stress) was correlated with AGEs-specific fluorescence. In patients this correlation was extremely significant (r = 0.8176, p < 0.0001).


CONCLUSIONS
The increased oxidative stress in children with type 1 diabetes may not be attributed to complications, though it could contribute to the development of complications. (Tab. 2, Fig. 7, Ref. 30.)
Jakus
V
V
Dpt of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Comenius University, Sasinkova 2, SK-811 08 Bratislava, Slovakia. exfakbau@nic.savba.sk
Bauerova
K
K
Michalkova
D
D
Carsky
J
J
D000293:Adolescent; D001798:Blood Proteins / Q000378:metabolism*; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000097:blood*; D019270:Fructosamine / Q000097:blood; D006442:Glycated Hemoglobin / Q000032:analysis; D017127:Glycation End Products, Advanced / Q000097:blood*; D006801:Humans; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000097:blood
D001798:Blood Proteins; D019270:Fructosamine; D006442:Glycated Hemoglobin; D017127:Glycation End Products, Advanced; D008315:Malondialdehyde