Bioelectrochemical analysis of neuropathy target esterase activity in blood.
Bioelectrochemical analysis of neuropathy target esterase (NTE) and its inhibitors is based on the combination of the NTE-catalyzed hydrolysis of phenyl valerate and phenol detection by a tyrosinase carbon-paste electrode. The use of the tyrosinase electrode improves 10-fold the sensitivity of NTE detection in comparison with a spectrophotometric method. The tyrosinase electrode was found to be suitable for measurements in whole human blood where spectrophotometric detection is considerably restricted. The specificity of NTE in blood for mipafox and di-2-propyl phosphorofluoridate was close to that for neuronal NTE. The NTE-like activity in blood was determined to be 0.19 +/- 0.02 nmol/min/mg of protein.
Sigolaeva
L V
LV
Faculty of Chemistry, M. V. Lomonosov Moscow State University, Moscow, 119899, Russia. ikur@genebee.msu.su
Makower
A
A
Eremenko
A V
AV
Makhaeva
G F
GF
Malygin
V V
VV
Kurochkin
I N
IN
Scheller
F W
FW
D000818:Animals; D015374:Biosensing Techniques; D001921:Brain / Q000201:enzymology*; D002265:Carboxylic Ester Hydrolases / Q000037:antagonists & inhibitors / Q000097:blood*; D004563:Electrochemistry / Q000379:methods*; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors / Q000378:metabolism; D010755:Organophosphates / Q000633:toxicity; D010261:Paraoxon / Q000494:pharmacology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013053:Spectrophotometry; D014631:Valerates / Q000378:metabolism
D004791:Enzyme Inhibitors; D010755:Organophosphates; D014631:Valerates; D014442:Monophenol Monooxygenase; D002265:Carboxylic Ester Hydrolases; C021859:neurotoxic esterase; D010261:Paraoxon