title: Phylogenetic relationship of Ophiostoma piliferum to other sapstain fungi based on the nuclear rRNA gene.
abstract: The nuclear rRNA gene of Ophiostoma piliferum was analyzed to understand its phylogenetic relationships to other sapstain fungi. Phylograms based on nucleotide sequences of the rRNA gene showed that the relationships between O. piliferum and other Ophiostoma species varied depending on the regions of the rRNA gene analyzed. Intraspecies variation in O. piliferum was found in the internal transcribed spacer regions, and the variation was related to the geographic origin of O. piliferum strains. A useful molecular marker for differentiating O. piliferum from other sapstain Ophiostoma species was generated by the HaeIII restriction fragment length polymorphism of the 26S rRNA gene.
authors.affiliation: Department of Geomicrobiology, ICBM, Carl von Ossietzky University of Oldenburg, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Schroeder
authors.forename: S H
authors.initials: SH
authors.lastname: Kim
authors.forename: W T
authors.initials: WT
authors.lastname: Cheung
authors.forename: K
authors.initials: K
authors.lastname: Sterflinger
authors.forename: C
authors.initials: C
authors.lastname: Breuil
mesh_terms: D001203:Ascomycota / Q000145:classification* / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005800:Genes, Fungal; D020459:Genes, rRNA; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012335:RNA, Ribosomal / Q000235:genetics
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S