title: Closely related HIV-1 CRF01_AE variant among injecting drug users in northern Vietnam: evidence of HIV spread across the Vietnam-China border.
abstract: To investigate the nature of recent HIV outbreaks among injecting drug users (IDUs) near the Vietnam-China border, we genetically analyzed 24 HIV-positive blood specimens from 2 northern provinces of Vietnam (Lang Son and quang Ninh) adjacent to the China border, where HIV outbreaks among IDUs were first detected in late 1996. Genetic subtyping based on gag (p17) and env (C2/V3) sequences revealed that CRF01_AE is a principal strain circulating throughout Vietnam, including the provinces near the China border. The majority of CRF01_AE sequences among IDUs in Quang Ninh and Lang Son showed significant clustering with those found in nearby Pingxiang City of China's Guangxi Province, sharing a unique valine substitution 12 amino acids downstream of the V3 loop. This particular subtype E variant, uniquely found among IDUs in northern Vietnam and southeastern China, is designated E(v). The genetic diversity of CRF01_AE distributed in Quang Ninh (1.5 +/- 0.6%) and Pingxiang City (1.9 +/- 1.2%) was remarkably low, indicating the emerging nature of HIV spread in these areas. It is also noted that the genetic diversity of CRF01_AE among IDUs was consistently lower than that in persons infected sexually, suggesting that fewer closely related CRF01_AE variants were introduced into IDUs and, conversely, that multiple strains of CRF01_AE had been introduced via the sexual route. The data in the present study provide additional evidence that HIV outbreaks among IDUs in northern Vietnam were caused by the recent introduction of a highly homogeneous CRF01_AE variant (E(v)) closely related to that prevailing in nearby southern China.
authors.affiliation: Laboratory of Molecular Virology and Epidemiology, AIDS Research Center, National Institute of Infectious Diseases, Tokyo 162-8640, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kato
authors.forename: S
authors.initials: S
authors.lastname: Kusagawa
authors.forename: K
authors.initials: K
authors.lastname: Motomura
authors.forename: R
authors.initials: R
authors.lastname: Yang
authors.forename: T
authors.initials: T
authors.lastname: Shiino
authors.forename: K
authors.initials: K
authors.lastname: Nohtomi
authors.forename: H
authors.initials: H
authors.lastname: Sato
authors.forename: K
authors.initials: K
authors.lastname: Shibamura
authors.forename: T H
authors.initials: TH
authors.lastname: Nguyen
authors.forename: K C
authors.initials: KC
authors.lastname: Pham
authors.forename: H T
authors.initials: HT
authors.lastname: Pham
authors.forename: C T
authors.initials: CT
authors.lastname: Duong
authors.forename: T H
authors.initials: TH
authors.lastname: Nguyen
authors.forename: D T
authors.initials: DT
authors.lastname: Bui
authors.forename: T L
authors.initials: TL
authors.lastname: Hoang
authors.forename: Y
authors.initials: Y
authors.lastname: Nagai
authors.forename: Y
authors.initials: Y
authors.lastname: Takebe
mesh_terms: D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D002681:China / Q000453:epidemiology; D003001:Cloning, Molecular; D004196:Disease Outbreaks; D005260:Female; D015752:Genes, env / Q000235:genetics; D015750:Genes, gag / Q000235:genetics; D016679:Genome, Viral; D015658:HIV Infections / Q000453:epidemiology / Q000821:virology*; D015497:HIV-1 / Q000145:classification / Q000235:genetics*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D012307:Risk Factors; D012725:Sexual Behavior; D015819:Substance Abuse, Intravenous / Q000821:virology*; D014633:Valine / Q000235:genetics; D014744:Vietnam / Q000453:epidemiology
chemical_list: D014633:Valine