title: Replication of the gonad-specific virus Hz-2V in Ld652Y cells mimics replication in vivo.
abstract: A newly discovered, nonoccluded insect virus, known as gonad-specific virus or Hz-2V, was found to replicate differently in two insect cell lines derived from ovarian tissues (Tn-368 cells from Trichoplusia ni and Ld652Y from Lymantria dispar). Differences between these two cell lines were observed in virus plaque forming ability, rate of viral DNA replication, time course of infectious virus production, and the mechanism of virus release from infected cells. Replication of Hz-2V in Ld652Y cells was more productive and more closely resembled in vivo virus replication.
authors.affiliation: Department of Medicine, Harvard Medical School, Cambridge, Massachusetts 02138, USA. jburand@microbio.umass.edu
authors.forename: H
authors.initials: H
authors.lastname: Lu
authors.forename: J P
authors.initials: JP
authors.lastname: Burand
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000254:growth & development*; D002460:Cell Line; D004261:DNA Replication; D007915:Lepidoptera / Q000821:virology*; D018716:Molecular Mimicry; D013997:Time Factors; D014776:Virus Cultivation / Q000379:methods*; D014779:Virus Replication