Fatty acid synthesis in developing leaves of Brassica napus in relation to leaf growth and changes in activity of 3-oxoacyl-ACP reductase.
In young expanding leaves of Brassica napus, the demand for fatty acids is met by de novo biosynthesis of fatty acid synthase components, as demonstrated by 3-oxoacyl-ACP reductase. Using a novel radio-chemical assay for 3-oxoacyl-ACP reductase and specific antibodies, we have demonstrated a direct relationship between the increase in activity and synthesis of polypeptide. The maximum rate of fatty acid synthesis was between 4 and 7 days post-emergence, but slowed after this point even though 3-oxoacyl-ACP reductase activity was high. Leaf area continued to expand in a linear fashion after reductions in both enzyme activity and the rate of fatty acid synthesis.
O'Hara
P
P
Department of Biological Sciences, University of Durham, DH1 3LE, Durham, UK.
Slabas
A R
AR
Fawcett
T
T
D064431:3-Oxoacyl-(Acyl-Carrier-Protein) Reductase; D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D001937:Brassica / Q000166:cytology / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism*; D002457:Cell Extracts; D005227:Fatty Acids / Q000032:analysis / Q000096:biosynthesis*; D007700:Kinetics; D018515:Plant Leaves / Q000166:cytology / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism*; D013053:Spectrophotometry; D014316:Tritium
D002457:Cell Extracts; D005227:Fatty Acids; D014316:Tritium; D000429:Alcohol Oxidoreductases; D064431:3-Oxoacyl-(Acyl-Carrier-Protein) Reductase