High-throughput reverse genetics: RNAi screens in Caenorhabditis elegans.
Two recent chromosome-wide screens for phenotypes caused by RNA-mediated interference (RNAi) in Caenorhabditis elegans have increased our understanding of essential genes in nematodes. These papers represent a major advance in functional genomics.
Bargmann
C I
CI
Howard Hughes Medical Institute, Departments of Anatomy and of Biochemistry and Biophysics, The University of California, San Francisco, CA 94143, USA. cori@itsa.ucsf.edu
D000818:Animals; D017173:Caenorhabditis elegans / Q000187:drug effects / Q000196:embryology / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000378:metabolism; D047108:Embryonic Development; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D017238:Genes, Helminth / Q000235:genetics; D010641:Phenotype; D012330:RNA, Double-Stranded / Q000235:genetics* / Q000494:pharmacology
D012330:RNA, Double-Stranded