title: Recognition of novel lipopolypeptides with many pendent sugar residues by lectin.
abstract: A lipid-polypeptide conjugate (lipo-polypeptide) was obtained by the ring-opening polycondensation of N-epsilon-Z-L-lysine N-carboxyanhydride (NCA) using 3-aminopropyl dioctadecylamine as initiator and subsequent deprotection. Maltose lactone was coupled with the lipo-polypeptide to give novel amphiphiles which carried many maltoamide residues as pendent groups. The sugar group-carrying amphiphiles incorporated in phospholipid liposomes were recognized by a lectin from Canavalia ensiformis (Con A), which was proven by the increase in turbidity of the liposome suspension after mixing with the lectin. The recognition was largely affected by the degree of polymerization of lysine residues and the surface density of the amphiphile in the liposomes. The association constant (K(ass)) of Con A with maltoamide residues on the liposome was much larger than those for small molecular weight sugars due to the "cluster effect".
authors.affiliation: Department of Chemical and Biochemical Engineering, Toyama University, Toyama 930-8555, Japan. kitano@eng.toyama-u.ac.jp
authors.forename: H
authors.initials: H
authors.lastname: Kitano
authors.forename: Y
authors.initials: Y
authors.lastname: Sumi
authors.forename: K
authors.initials: K
authors.lastname: Tagawa
mesh_terms: D002241:Carbohydrates / Q000032:analysis* / Q000737:chemistry; D003208:Concanavalin A / Q000737:chemistry; D008027:Light; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes; D009391:Nephelometry and Turbidimetry; D010455:Peptides / Q000737:chemistry*; D010743:Phospholipids; D012542:Scattering, Radiation
chemical_list: D002241:Carbohydrates; D008055:Lipids; D008081:Liposomes; D010455:Peptides; D010743:Phospholipids; D003208:Concanavalin A