title: Interaction of alpha-amylase with n-alkylammonium bromides.
abstract: The interaction of alpha-amylase with n-alkylammonium bromides above and below their critical micellar concentrations (cmc) has been studied in buffer at pH 7 and 10 by UV spectrophotometry, photon correlation spectroscopy and Doppler microelectrophoresis. This interaction produces a complex that is dependent on pH of the medium. This complex appears at surfactant concentrations below the cmc, which means that individual surfactant molecules can bind tightly to native alpha-amylase. The complex maintains its aggregation state when the concentration of surfactants with a hydrocarbon chain of 16 carbons increases, but not for surfactants of 12 and 14 carbons. Measurements of zeta-potential indicate the influence of electrostatic and hydrophobic forces. When the size of the aggregate is maximal, proteins are at their point of zero charge. In such conditions, Van der Waals forces and contacts between the alkyl chain and the hydrophobic core of the protein favour the formation of a larger aggregate.
authors.affiliation: Departament de Fisicoquímica, Facultat de Farmàcia, Universitat de Barcelona, Avda. Joan XXIII s/n, 08028, Barcelona, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Sabaté
authors.forename: J
authors.initials: J
authors.lastname: Estelrich
mesh_terms: D001965:Bromides / Q000737:chemistry* / Q000378:metabolism; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008823:Micelles; D009391:Nephelometry and Turbidimetry; D017785:Photons; D011485:Protein Binding; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D013053:Spectrophotometry; D013501:Surface-Active Agents / Q000737:chemistry; D013696:Temperature; D014466:Ultraviolet Rays; D000516:alpha-Amylases / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001965:Bromides; D002593:Cetrimonium Compounds; D008823:Micelles; D000644:Quaternary Ammonium Compounds; D013501:Surface-Active Agents; D000516:alpha-Amylases; C022806:dodecyltrimethylammonium; C051470:ammonium bromide; D000077286:Cetrimonium