Anti-8-oxo-2'-deoxyguanosine phage antibodies: isolation, characterization, and relationship to disease states.
We have used human single chain Fv (scFv) phage display antibody libraries to isolate recombinant antibodies against the DNA adduct 8-oxo-2'-deoxyguanosine (8-oxodG). One of these scFvs (175G) bound to several 8-oxodG-containing oligonucleotides whilst demonstrating no cross-reactivity with G-containing control oligonucleotides, and bound to 8-oxodG lesions introduced into DNA by treatment with methylene blue and white light. In addition, 175G inhibited the cleavage of an 8-oxodG-containing oligonucleotide by the Escherichia coli enzyme formamidopyrimidine-DNA glycosylase (Fpg). The nucleotide sequence of the 175G V(H) gene segment was 98% homologous to the published V(H) sequence of a human hybridoma derived from a patient with systemic lupus erythematosus (SLE). Sera from two SLE patients bound to damaged DNA, and this binding could be inhibited by 175G. The use of human scFv phage display libraries has thus produced a unique reagent with specificity for 8-oxodG, which may have a role in damage detection and quantitation and in modifying DNA repair activity. 175G also offers support to the hypothesis that SLE might be associated with oxidative damage to DNA.
Seedhouse
C H
CH
Department of Immunology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester, M20 4BX, United Kingdom.
Margison
G P
GP
Hendry
J H
JH
Hajeer
A
A
Embleton
M J
MJ
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000595:Amino Acid Sequence; D000906:Antibodies / Q000235:genetics / Q000302:isolation & purification*; D000918:Antibody Specificity; D001483:Base Sequence; D003001:Cloning, Molecular; D004249:DNA Damage; D004260:DNA Repair; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors*; D006801:Humans; D007128:Immunoglobulin Fragments / Q000235:genetics / Q000302:isolation & purification; D008180:Lupus Erythematosus, Systemic / Q000276:immunology / Q000378:metabolism; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000235:genetics / Q000276:immunology; D019151:Peptide Library
D000906:Antibodies; D007128:Immunoglobulin Fragments; D009838:Oligodeoxyribonucleotides; D019151:Peptide Library; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine