Construction of high-complexity combinatorial phage display peptide libraries.
Random peptide libraries displayed on the surface of filamentous bacteriophage are widely used as tools for the discovery of ligands for biologically relevant macromolecules, including antibodies, enzymes, and cell surface receptors. Phage display results in linkage of an affinity-selectable function (the displayed peptide) to the DNA encoding that function, allowing selection of individual binding clones by iterative cycles of in vitro panning and in vivo amplification. Critical to the success of a panning experiment is the complexity of the library: the greater the diversity of clones within the library, the more likely the library contains sequences that will bind a given target with useful affinity. A method for construction of high-complexity (> or = 10(9) independent clones) random peptide libraries is presented. The key steps are highly efficient binary ligation under conditions where the vector is relatively dilute, with only a modest molar excess of insert, followed by efficient electrotransformation into Escherichia coli. Library design strategies and a protocol for rapid sequence characterization are also presented.
Noren
K A
KA
New England Biolabs, 32 Tozer Road, Beverly, Massachusetts 01915, USA.
Noren
C J
CJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D018604:Epitope Mapping / Q000379:methods; D004926:Escherichia coli; D015723:Gene Library; D005821:Genetic Techniques; D007700:Kinetics; D008969:Molecular Sequence Data; D019151:Peptide Library; D010957:Plasmids / Q000378:metabolism; D014162:Transfection
D019151:Peptide Library; D004247:DNA