High expression of HLA-A2 on an oral squamous cell carcinoma with down-regulated transporter for antigen presentation.
Using the reverse transcription PCR, we evaluated expression levels of various antigen presentation-related genes, including LMP2, LMP7, MECL-1, PA28alpha, PA28beta, TAP1, TAP2, and tapasin, in two oral squamous cell carcinoma cell lines, HSC5 and HSC7. Expression levels of LMP2, MECL-1, TAP1, and TAP2 transcripts are reduced in both cell lines in comparison with a normal epithelial cell line. Further, HSC5 and HSC7 show diminished expression of LMP7/tapasin, and PA28alpha/beta, respectively. Surface expression of HLA-B alleles is down-regulated in both lines presumably due to low expression of TAP1/2. However, HLA-A2 surface expression is not significantly down-regulated in HSC5 cells, suggesting an involvement of signal-sequence derived peptides in the TAP-independent pathway. The current study would contribute to our understanding of significance of abnormalities in the antigen presentation machinery of oral squamous cell carcinoma, and provide meaningful information in the design of CTL-based tumor vaccines by intra-cellular delivery system.
Matsui
M
M
Department of Microbiology, Saitama Medical School, Moroyama-Cho, Iruma-Gun, Saitama, 350-0495, Japan.
Ikeda
M
M
Akatsuka
T
T
D017951:Antigen Presentation; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D002460:Cell Line; D018076:DNA, Complementary / Q000378:metabolism; D015536:Down-Regulation; D004848:Epithelium / Q000378:metabolism; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D015789:HLA-A2 Antigen / Q000096:biosynthesis*; D007371:Interferon-gamma / Q000494:pharmacology; D009062:Mouth Neoplasms / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D013602:T-Lymphocytes, Cytotoxic / Q000378:metabolism; D013997:Time Factors; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D015789:HLA-A2 Antigen; D012333:RNA, Messenger; D007371:Interferon-gamma