title: Heterogeneous lack of expression of the tumour suppressor PTEN protein in human neoplastic tissues.
abstract: PTEN, a tumour suppressor gene located at chromosome 10q23 and commonly mutated or deleted in a variety of tumours, encodes a dual-specific/phosphatidylinositol-3,4,5-triphosphate (PIP3) phosphatase. We report the generation of an anti-PTEN monoclonal antibody (MAb) that recognises an epitope at the C-terminus of PTEN, and describe the heterogeneous lack of expression of the PTEN protein in human tumour tissues, as demonstrated by immunohistochemical methods. Our anti-PTEN MAb provides a useful tool for the study of PTEN protein expression in tumour samples, in the search for tumour prognostic molecular markers.
authors.affiliation: Instituto de Investigaciones Citológicas, Amadeo de Saboya 4, 46010, Valencia, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Torres
authors.forename: S
authors.initials: S
authors.lastname: Navarro
authors.forename: I
authors.initials: I
authors.lastname: Roglá
authors.forename: F
authors.initials: F
authors.lastname: Ripoll
authors.forename: A
authors.initials: A
authors.lastname: Lluch
authors.forename: J
authors.initials: J
authors.lastname: García-Conde
authors.forename: A
authors.initials: A
authors.lastname: Llombart-Bosch
authors.forename: J
authors.initials: J
authors.lastname: Cervera
authors.forename: R
authors.initials: R
authors.lastname: Pulido
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D009363:Neoplasm Proteins / Q000378:metabolism*; D009369:Neoplasms / Q000175:diagnosis*; D051059:PTEN Phosphohydrolase; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins
chemical_list: D000911:Antibodies, Monoclonal; D009363:Neoplasm Proteins; D025521:Tumor Suppressor Proteins; D010744:Phosphoric Monoester Hydrolases; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human