title: Lack of mutation at codon 531 of SRC in advanced colorectal cancers from Italian patients.
abstract: A truncating mutation (C to T transition) at codon 531 of the human protooncogene c-src, possibly accounting for the activation of c-src tyrosine kinase, has been recently identified in a subset of advanced colorectal cancer from North-American patients. However, two subsequent studies have failed to confirm the occurrence of SRC 531 mutation in colorectal cancers from North-European and Asiatic patients, raising the hypothesis that the genetic activation of src in colon cancer might be restricted to patients belonging to specific ethnic groups. We investigated a large series of colorectal cancers from Italian patients (155 cases) with a high prevalence of liver metastasis (43%). Using a PCR-RFLP assay, the occurrence of a SRC 531 mutation was ruled out in all the investigated specimens of primary tumours and/or metastases. Our results demonstrate that SRC Gln531AMB plays no role in the development or in the progression of colorectal cancer among Italian patients.
authors.affiliation: Department of Gastroenterology, Istituto Clinico Humanitas, Via Manzoni 56, Rozzano, Milan, 20089, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Laghi
authors.forename: P
authors.initials: P
authors.lastname: Bianchi
authors.forename: O
authors.initials: O
authors.lastname: Orbetegli
authors.forename: L
authors.initials: L
authors.lastname: Gennari
authors.forename: M
authors.initials: M
authors.lastname: Roncalli
authors.forename: A
authors.initials: A
authors.lastname: Malesci
mesh_terms: D001483:Base Sequence; D003062:Codon / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000737:chemistry / Q000235:genetics; D016391:Genes, src / Q000235:genetics*; D006801:Humans; D007558:Italy; D009154:Mutation; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D003062:Codon; D004273:DNA, Neoplasm