Interleukin 1 beta (IL-1 beta) action in porcine thyroid cells involves the ceramide signalling pathway.
Interleukin 1 beta (IL-1beta) is often associated with thyroidal autoimmune diseases. This cytokine has been largely described to trigger an important biological signalling pathway: the sphingomyelin/ceramide pathway. In this report we show that IL-1beta induces ceramide formation and sphingomyelin degradation in porcine thyroid cells via the activation of a neutral sphingomyelinase. Among the potential targets of IL-1beta and ceramides action, we have investigated the role of an atypical protein kinase C (PKC), the PKC zeta. We show that both IL-1beta and ceramides lead to an increase of PKCzeta activity. All these results suggest an important role for ceramides and IL-1beta in regulation of thyroid function, leading to cell survival or to apoptosis.
Schneider
C
C
IFR 53 Biomolécules UPRES-A CNRS 6021 "Signalisation cellulaire et matrice extracellulaire" UFR de Médecine et de Sciences, Université de Reims Champagne-Ardenne, Reims, France.
Delorme
N
N
El Btaouri
H
H
Hornebeck
W
W
Haye
B
B
Martiny
L
L
D000818:Animals; D002478:Cells, Cultured; D002518:Ceramides / Q000502:physiology*; D007375:Interleukin-1 / Q000502:physiology*; D007527:Isoenzymes / Q000502:physiology; D011493:Protein Kinase C / Q000502:physiology; D015398:Signal Transduction / Q000276:immunology*; D013109:Sphingomyelins / Q000502:physiology; D013552:Swine; D013961:Thyroid Gland / Q000166:cytology / Q000201:enzymology / Q000276:immunology*; D000099221:Protein Kinase C zeta
D002518:Ceramides; D007375:Interleukin-1; D007527:Isoenzymes; D011493:Protein Kinase C; D013109:Sphingomyelins; D000099221:Protein Kinase C zeta