The nitroxide Tempol affords protection against ultraviolet radiation in a transgenic murine fibroblast culture model of cutaneous photoaging.
The generation of reactive oxygen species is among the various mechanisms by which ultraviolet radiation damages skin. Tempol, a superoxide dismutase analogue which readily penetrates cell membranes when administered exogenously, has been shown to provide protection against some forms of oxygen-dependent damage. In this study, we measured the ability of Tempol to protect against ultraviolet A- and ultraviolet B-induced damage, using a previously described transgenic mouse model of cutaneous photoaging. The ability of Tempol to prevent ultraviolet radiation-induced elastin promoter activation was determined in vitro. Tempol provided over 50% protection against ultraviolet B and over 70% protection against ultraviolet A as measured in our in vitro system. These results demonstrate the ability of the superoxide dismutase mimic, Tempol, to protect against ultraviolet induced elastin promoter activation. This compound could be a useful pharmacological agent to prevent cutaneous photoaging.
Bernstein
E F
EF
Department of Dermatology and Cutaneous Biology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107, USA.
Kong
S K
SK
Brown
D B
DB
Kwak
B C
BC
Takeuchi
T
T
Gasparro
F P
FP
Uitto
J
J
D000818:Animals; D002478:Cells, Cultured; D003497:Cyclic N-Oxides / Q000494:pharmacology*; D004549:Elastin / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D016166:Free Radical Scavengers / Q000494:pharmacology*; D051379:Mice; D008822:Mice, Transgenic; D008954:Models, Biological; D011835:Radiation Protection / Q000379:methods; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D015595:Skin Aging / Q000187:drug effects* / Q000528:radiation effects; D013113:Spin Labels; D013482:Superoxide Dismutase / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects
D003497:Cyclic N-Oxides; D016166:Free Radical Scavengers; D011837:Radiation-Protective Agents; D013113:Spin Labels; D004549:Elastin; D013482:Superoxide Dismutase; C001803:tempol