title: Boursin, a sea urchin bimC kinesin protein, plays a role in anaphase and cytokinesis.
abstract: We have isolated and characterized Boursin, a kinesin-related protein of the bimC family, from Paracentrotus lividus sea urchin eggs. Boursin is expressed at high levels in eggs and embryos during early cleavage stages. Boursin was found to be associated with different parts of the mitotic spindle from early prophase to telophase. Expression of a form of the protein predicted to act as a dominant negative mutant caused severe defects in cell division and resulted in the formation of embryos with polyploid and multiastral blastomeres. Immunofluorescence analysis indicated that these defects did not arise from failure in either centrosome separation or bipolar spindle formation. Time-lapse observations showed rather that these perturbations in cell division resulted from abnormal anaphase and failure to complete cytokinesis. These phenotypes differ from the phenotype described following perturbation of the function of bimC family members in other organisms. Our study has thus uncovered roles for a bimC kinesin in late stages of cell division.
authors.affiliation: UMR 7009 CNRS-UPMC, Observatoire Oceanologique, 06234 Villefranche-Sue-Mer Cedex, France. touitou@obs-vlfr.fr
authors.forename: I
authors.initials: I
authors.lastname: Touïtou
authors.forename: G
authors.initials: G
authors.lastname: Lhomond
authors.forename: G
authors.initials: G
authors.lastname: Prulière
mesh_terms: D000595:Amino Acid Sequence; D000705:Anaphase / Q000502:physiology*; D000818:Animals; D001483:Base Sequence; D002453:Cell Cycle / Q000502:physiology*; D017931:DNA Primers; D005260:Female; D016547:Kinesins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D009154:Mutation; D010063:Ovum / Q000378:metabolism
chemical_list: D017931:DNA Primers; D016547:Kinesins