Occurrence and virulence factors of non-O157 Shiga toxin-producing Escherichia coli in retail meat in Dunedin, New Zealand.
Retail raw meat was sampled for the presence of Shiga toxin-producing Escherichia coli (STEC) using enrichment culture and Vero cell assay. The STEC obtained were serotyped and tested for enterohaemolysin (Ehly) production and the eae gene. The presence of Shiga toxin genes (stx) was confirmed by polymerase chain reaction. A total of 18 STEC were isolated accounting for 12% of beef, 17% of lamb and 4% of pork samples. Five isolates produced Ehly but none possessed the eae gene. Five isolates were identified which possessed the stx2 gene and belonged to serotypes associated with severe infection.
Brooks
H J
HJ
Department of Microbiology, Otago School of Medical Sciences, University of Otago, Dunedin, New Zealand. heather.brooks@stonebow.otago.ac.nz
Mollison
B D
BD
Bettelheim
K A
KA
Matejka
K
K
Paterson
K A
KA
Ward
V K
VK
D018829:Adhesins, Bacterial; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D002352:Carrier Proteins; D002417:Cattle; D002645:Chickens; D004926:Escherichia coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D006460:Hemolysin Proteins / Q000096:biosynthesis; D008460:Meat / Q000382:microbiology*; D009520:New Zealand; D012703:Serotyping; D012756:Sheep; D022603:Shiga Toxins / Q000096:biosynthesis* / Q000235:genetics; D013552:Swine; D014774:Virulence
D018829:Adhesins, Bacterial; D001425:Bacterial Outer Membrane Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; D022603:Shiga Toxins; C066219:enterohemolysin; C073819:eaeA protein, E coli