Hypofibronectinaemia and severity of sickle cell anaemia in Nigeria.
Plasma fibronectin levels in sickle cell anaemia and apparently healthy Nigerians were investigated to determine any correlation with disease severity. A cheaper in-house plasma fibronectin assay was also developed that could be adapted for use in Africa and elsewhere. Plasma fibronectin assay was concurrently carried out using the newly developed inhibition enzyme-linked immunosorbent assay (ELISA) and a commercial competitive binding ELISA. The in-house assay compared favourably with that of the commercial kit. The mean plasma fibronectin levels in sickle cell anaemia subjects were significantly lower than that of control subjects (P < 0.001). Plasma fibronectin concentration could therefore be useful in assessing the severity of sickle cell anaemia.
Emeribe
A O
AO
Department of Haematology, University of Calabar, Nigeria. gbis@alpha.linkserve.com
Udoh
A E
AE
Etukudoh
M H
MH
Okany
C C
CC
Kehinde
M
M
Catty
D
D
Raykundalia
C
C
D000293:Adolescent; D000328:Adult; D000755:Anemia, Sickle Cell / Q000097:blood*; D001667:Binding, Competitive; D015415:Biomarkers / Q000097:blood; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005353:Fibronectins / Q000097:blood*; D006801:Humans; D009549:Nigeria; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index
D015415:Biomarkers; D005353:Fibronectins