Antigenotoxic properties of selenium: studies in the wing spot test in Drosophila.
The genotoxic activity of three selenium compounds (sodium selenite, sodium selenate, and selenious acid) and the antigenotoxic effects of sodium selenite in combination with the chromium compound potassium dichromate were studied using the wing spot test of Drosophila melanogaster. This assay is based on the principle that the loss of heterozygosity of suitable recessive markers, multiple wing hairs (mwh) and flare-3 (flr[3]), can lead to the formation of mutant clones of larval cells, which are then expressed as spots on the wings of the adult flies. Pretreatment and chronic cotreatment was comparatively used for the antigenotoxicity study. From the results obtained, it was evident that all selenium compounds are unable to increase the frequency of any of the three categories of spots recorded (small, large, and twin spots). Nevertheless, the antigenotoxic effects of sodium selenite were clearly demonstrated, in both cotreatment and pretreatment, by a complete suppression of those clones induced by potassium dichromate. Therefore, the D. melanogaster wing spot test was revealed to be a good assay, not only for evaluating genotoxic activity but also for detecting antigenotoxic effects in vivo.
Rizki
M
M
Grup de Mutagènesi, Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Amrani
S
S
Creus
A
A
Xamena
N
N
Marcos
R
R
D000818:Animals; D004330:Drosophila; D004357:Drug Synergism; D009152:Mutagenicity Tests; D011192:Potassium Dichromate / Q000494:pharmacology / Q000633:toxicity; D015203:Reproducibility of Results; D064586:Selenic Acid; D020887:Selenious Acid / Q000633:toxicity*; D018036:Selenium Compounds / Q000633:toxicity*; D018038:Sodium Selenite / Q000494:pharmacology* / Q000633:toxicity*; D014921:Wings, Animal / Q000187:drug effects* / Q000473:pathology
D018036:Selenium Compounds; D020887:Selenious Acid; D018038:Sodium Selenite; D064586:Selenic Acid; D011192:Potassium Dichromate