Diets enriched with cereal brans or inulin modulate protein kinase C activity and isozyme expression in rat colonic mucosa.
The role of dietary fibres in colon carcinogenesis is controversial. To elucidate the mechanisms by which different dietary fibre sources may affect colonic tumour development, we studied the effects of diets enriched with cereal brans or inulin on protein kinase C (PKC) activity and isozyme expression in rat colon. Male Wistar rats (twelve per group) were fed one of the following AIN-93G-based diets (Reeves et al. 1993) for 4 weeks: a non-fibre high-fat diet or one of the four high-fat diets supplemented with either rye, oat or wheat bran or inulin at 100 g/kg diet. The fat concentration (20 g/100 g) and fatty acid composition of the non-fibre high-fat diet was designed to approximate that in a typical Western-type diet. In the proximal colon, rats fed the inulin diet had a significantly higher membrane PKC activity and a higher membrane PKC delta level than rats fed the non-fibre diet In the distal colon, rats fed the inulin and oat bran diets had a higher total PKC activity and a higher membrane PKC beta 2 level than rats fed the wheat-bran diet. Rats in the non-fibre and wheat-bran groups had the lowest concentrations of luminal diacylglycerol. In conclusion, feeding of wheat bran resulted in low distal PKC activity and expression of PKC beta 2, a PKC isozyme related to colonic cell proliferation and increased susceptibility for colon carcinogenesis, which may explain in part the protective effect of wheat bran against tumour development in a number of experimental colon cancer studies. The increase in PKC activity and PKC beta 2 expression by feeding inulin may be a drawback of inulin as a functional food.
Pajari
A M
AM
Department of Applied Chemistry and Microbiology, Nutrition, PO Box 27, 00014 University of Helsinki, Finland. anne-marie.pajari@helsinki.fi
Oikarinen
S
S
Gråsten
S
S
Mutanen
M
M
D000818:Animals; D003106:Colon / Q000201:enzymology*; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000201:enzymology / Q000517:prevention & control; D004043:Dietary Fiber / Q000008:administration & dosage / Q000494:pharmacology*; D004075:Diglycerides / Q000032:analysis; D002523:Edible Grain; D015151:Immunoblotting; D007413:Intestinal Mucosa / Q000201:enzymology; D007444:Inulin / Q000008:administration & dosage / Q000633:toxicity*; D007527:Isoenzymes / Q000378:metabolism*; D008297:Male; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D004043:Dietary Fiber; D004075:Diglycerides; D007527:Isoenzymes; D007444:Inulin; D011493:Protein Kinase C