Absence of rearrangements in the BRCA2 gene in human cancers.
Mutations of BRCA2 in sporadic breast and ovarian carcinomas are exceedingly rare. This led to the suggestion that large genomic rearrangements could be involved. We performed Southern blots in genomic DNA from 130 primary breast cancers and 83 cancer cell lines (breast, ovarian, pancreatic and small cell lung carcinomas) and found no genomic rearrangements. These results suggest that a gene other than BRCA2 is the target of the frequent 13q12.3 allelic deletions in human cancers.
Chin
S F
SF
CRC Department of Oncology, University of Cambridge and Cambridge Institute for Medical Research/Wellcome Trust Centre for Molecular Mechanisms in Disease, Box 139, Addenbrookes Hospital, Hills Road, Cambridge, CB2 2XY, UK.
Wang
Q
Q
Puisieux
A
A
Caldas
C
C
D024682:BRCA2 Protein; D015139:Blotting, Southern; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D015321:Gene Rearrangement; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D014157:Transcription Factors / Q000235:genetics*; D014407:Tumor Cells, Cultured
D024682:BRCA2 Protein; D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D014157:Transcription Factors