DNA vaccine-mediated immune responses in Coxsackie virus B3-infected mice.
DNA immunizations with the major structural protein VP1 of Coxsackie virus B3 (CVB3) have been previously found to protect BALB/c mice from lethal challenge. Here we report that the other CVB3 capsid proteins, VP2, VP3, and VP4, were less effective at preventing CVB3-caused disease. The application of pCMV/VP1 as a vaccine caused decreased myocyte destruction, reduced viral load in the heart tissue, accelerated antibody induction, and an early cytokine expression in heart tissue. In summary, our results indicate that the induction of B cell and/or T cell memory in vaccinated mice prior to challenge is responsible for the protection observed.
Henke
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Institute of Virology, Medical Center, Friedrich Schiller University, Winzerlaer Str. 10, D-07745 Jena, Germany. i6hean@rz.uni-jena.de
Zell
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Stelzner
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D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D001835:Body Weight; D002213:Capsid / Q000235:genetics; D003384:Coxsackievirus Infections / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D016207:Cytokines / Q000032:analysis / Q000276:immunology; D018572:Disease-Free Survival; D004770:Enterovirus / Q000235:genetics / Q000276:immunology*; D005822:Genetic Vectors; D006321:Heart / Q000821:virology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009206:Myocardium / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology; D019562:Viral Load
D000914:Antibodies, Viral; D016207:Cytokines; D012333:RNA, Messenger; D019444:Vaccines, DNA