title: Interleukin 20: discovery, receptor identification, and role in epidermal function.
abstract: A structural, profile-based algorithm was used to identify interleukin 20 (IL-20), a novel IL-10 homolog. Chromosomal localization of IL-20 led to the discovery of an IL-10 family cytokine cluster. Overexpression of IL-20 in transgenic (TG) mice causes neonatal lethality with skin abnormalities including aberrant epidermal differentiation. Recombinant IL-20 protein stimulates a signal transduction pathway through STAT3 in a keratinocyte cell line, demonstrating a direct action of this ligand. An IL-20 receptor was identified as a heterodimer of two orphan class II cytokine receptor subunits. Both receptor subunits are expressed in skin and are dramatically upregulated in psoriatic skin. Taken together, these results demonstrate a role in epidermal function and psoriasis for IL-20, a novel cytokine identified solely by bioinformatics analysis.
authors.affiliation: Department of Genetics, ZymoGenetics, Inc., 1201 Eastlake Avenue E, Seattle, WA 98102, USA.
authors.forename: H
authors.initials: H
authors.lastname: Blumberg
authors.forename: D
authors.initials: D
authors.lastname: Conklin
authors.forename: W F
authors.initials: WF
authors.lastname: Xu
authors.forename: A
authors.initials: A
authors.lastname: Grossmann
authors.forename: T
authors.initials: T
authors.lastname: Brender
authors.forename: S
authors.initials: S
authors.lastname: Carollo
authors.forename: M
authors.initials: M
authors.lastname: Eagan
authors.forename: D
authors.initials: D
authors.lastname: Foster
authors.forename: B A
authors.initials: BA
authors.lastname: Haldeman
authors.forename: A
authors.initials: A
authors.lastname: Hammond
authors.forename: H
authors.initials: H
authors.lastname: Haugen
authors.forename: L
authors.initials: L
authors.lastname: Jelinek
authors.forename: J D
authors.initials: JD
authors.lastname: Kelly
authors.forename: K
authors.initials: K
authors.lastname: Madden
authors.forename: M F
authors.initials: MF
authors.lastname: Maurer
authors.forename: J
authors.initials: J
authors.lastname: Parrish-Novak
authors.forename: D
authors.initials: D
authors.lastname: Prunkard
authors.forename: S
authors.initials: S
authors.lastname: Sexson
authors.forename: C
authors.initials: C
authors.lastname: Sprecher
authors.forename: K
authors.initials: K
authors.lastname: Waggie
authors.forename: J
authors.initials: J
authors.lastname: West
authors.forename: T E
authors.initials: TE
authors.lastname: Whitmore
authors.forename: L
authors.initials: L
authors.lastname: Yao
authors.forename: M K
authors.initials: MK
authors.lastname: Kuechle
authors.forename: B A
authors.initials: BA
authors.lastname: Dale
authors.forename: Y A
authors.initials: YA
authors.lastname: Chandrasekher
mesh_terms: D000818:Animals; D002460:Cell Line; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000378:metabolism; D019281:Dimerization; D004817:Epidermis / Q000737:chemistry / Q000276:immunology* / Q000473:pathology; D015870:Gene Expression / Q000276:immunology; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics / Q000276:immunology; D007378:Interleukins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D015603:Keratinocytes / Q000166:cytology / Q000276:immunology; D007633:Keratins / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D011565:Psoriasis / Q000276:immunology / Q000473:pathology; D018121:Receptors, Cytokine / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D050796:STAT3 Transcription Factor; D017386:Sequence Homology, Amino Acid; D015534:Trans-Activators / Q000378:metabolism; D015854:Up-Regulation / Q000276:immunology
chemical_list: D004268:DNA-Binding Proteins; D007378:Interleukins; D018121:Receptors, Cytokine; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; C494087:Stat3 protein, mouse; D015534:Trans-Activators; D016753:Interleukin-10; D007633:Keratins; C422042:interleukin 20