title: Connexin 26 distribution in gap junctions between melanocytes in the human vestibular dark cell area.
abstract: We have previously demonstrated the presence of gap junctions between melanocytes in the human vestibular organ and have speculated that melanocytes function in maintaining the homeostasis of the microenvironment of the inner ear. The purpose of the present study was to characterize the expression and ultrastructural localization of connexin (Cx) protein in melanocytes of the human vestibular organs. Surgical material was obtained from patients operated on for vestibular schwannoma and was processed for light microscopy, confocal laser scanning microscopy, conventional TEM, and immuno TEM. The specimens were labeled with anti-Cx26, Cx32, and Cx43 antibodies and examined by light microscopy. Specimens were also labeled with anti-Cx26 antibody and examined by laser microscopy and immuno-TEM methods. The specimens examined in this study were mainly dark cell areas from the human vestibular organ, whose epithelial and subepithelial layers are rich in melanocytes. Light-microscopic immunohistochemical studies showed positive labeling for Cx26 protein between subepithelial melanocytes, and Cx32 was also detected. Use of anti-Cx26 antibody and confocal laser scanning microscopy revealed high levels of Cx26 around the subepithelial melanocytes. Post-embedding immuno-gold transmission electron microscopy showed significant aggregation of gold particles (33.97 +/- 8.01% of total gold particles) around the gap junctions of the subepithelial melanocytes. The results of this study indicated that melanocytes are connected through gap junctions that mainly contain Cx26. This suggested that the melanocytes in the human vestibular organ may play a role in transporting material between the endolymph and perilymph.
authors.affiliation: Department of Otorhinolaryngology, Keio University, School of Medicine, Tokyo, Japan. mentkeio@ea.mbn.or.jp
authors.forename: M
authors.initials: M
authors.lastname: Masuda
authors.forename: S
authors.initials: S
authors.lastname: Usami
authors.forename: K
authors.initials: K
authors.lastname: Yamazaki
authors.forename: Y
authors.initials: Y
authors.lastname: Takumi
authors.forename: H
authors.initials: H
authors.lastname: Shinkawa
authors.forename: K
authors.initials: K
authors.lastname: Kurashima
authors.forename: T
authors.initials: T
authors.lastname: Kunihiro
authors.forename: J
authors.initials: J
authors.lastname: Kanzaki
mesh_terms: D000328:Adult; D000368:Aged; D000072259:Connexin 26; D018031:Connexin 43 / Q000032:analysis; D017630:Connexins / Q000032:analysis*; D005260:Female; D017629:Gap Junctions / Q000737:chemistry* / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008544:Melanocytes / Q000737:chemistry* / Q000648:ultrastructure; D008854:Microscopy, Electron; D008875:Middle Aged; D014722:Vestibule, Labyrinth / Q000737:chemistry* / Q000648:ultrastructure; D000097002:Gap Junction beta-1 Protein
chemical_list: D000072259:Connexin 26; D018031:Connexin 43; D017630:Connexins; D000097002:Gap Junction beta-1 Protein; C000607042:GJB2 protein, human