Identification of a novel DRB1 allele, DRB1*1112, by sequence-based DRB typing.
We report here a novel DRB1 allele (DRB1*1112) identified during sequence-based HLA-DRB typing. Polymerase chain reaction with generic DRB primers and group-specific primers and subsequent sequencing yielded identical results. Molecular cloning and sequencing confirmed that the new DRB1 allele is identical to DRB1*11011 and 1129 at exon 2 except for a single nucleotide substitution at codon 37, changing the codon from Tyr (DRB1*11011) or Ser (DRB1*1129) to Phe.
Luo
M
M
Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada.
Blanchard
J
J
Maclean
I
I
Brunham
R
R
D000368:Aged; D000483:Alleles; D001483:Base Sequence; D006684:HLA-DR Antigens / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D044465:White People / Q000235:genetics
D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; C553518:HLA-DRB1*11:01:01 antigen; C553515:HLA-DRB1*12:12 antigen