title: [Effect of mutations in PHO85 and PHO4 genes on utilization of proline in Saccharomyces cerevisiae yeasts].
abstract: Yeast Saccharomyces cerevisiae can utilize proline as a single nitrogen source. We have found that the strains with mutations in gene PH085, a structural gene encoding cyclin-dependent phosphoprotein kinase, cannot grow on the proline-containing media. The ability to utilize proline is restored in strains of the pho85pho4 genotype. We suggest that phosphoprotein kinase Pho85p is involved in either phosphorylation of a highly specific proline permease, Put4p, or in signaling proline concentration. The Pho4p protein that activates transcription of the PH05 gene, a structural gene of acid phosphatase, seems to participate in the negative regulation of the PUT1 and PUT2 genes encoding enzymes of proline catabolism, proline oxidase and delta-pyrroline-5-carboxylate dehydrogenase. Thus, regulation of phosphorus and nitrogen metabolism have common elements.
authors.affiliation: Biological Research Institute, St. Petersburg State University, St. Petersburg, 198904 Russia.
authors.forename: Iu G
authors.initials: IuG
authors.lastname: Popova
authors.forename: M V
authors.initials: MV
authors.lastname: Padkina
authors.forename: E V
authors.initials: EV
authors.lastname: Sambuk
mesh_terms: D000135:Acid Phosphatase / Q000235:genetics*; D001483:Base Sequence; D001692:Biological Transport; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009154:Mutation; D010766:Phosphorylation; D011392:Proline / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*
chemical_list: D011392:Proline; D000135:Acid Phosphatase