SRC transcriptional activation in a subset of human colon cancer cell lines.
Human SRC encodes the non-receptor tyrosine kinase pp60(c-Src), which is activated in many human colon cancer cell lines (HCCLs) and tumors. We found that both c-Src protein and mRNA levels were elevated in a subset of HCCLs. Increased c-Src mRNA and protein levels correlated strongly with increased c-Src kinase activity. Nuclear run-on analysis and c-Src mRNA half-life determination demonstrated increased mRNA levels were due to increased transcription of the SRC gene. We also observed decreased c-Src mRNA stability in cell lines that displayed SRC transcriptional activation. Our findings provide the first evidence that SRC transcriptional activation is an important determinant of c-Src expression and activity in HCCLs.
Dehm
S
S
Department of Biochemistry, University of Saskatchewan, Saskatoon, Canada.
Senger
M A
MA
Bonham
K
K
D003110:Colonic Neoplasms / Q000201:enzymology* / Q000235:genetics*; D016391:Genes, src; D019073:HT29 Cells; D006801:Humans; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D015533:Transcriptional Activation; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D012334:RNA, Neoplasm; D016392:Proto-Oncogene Proteins pp60(c-src)