Expression of adhesion and activation molecules in human buccal epithelial cell lines and normal human buccal epithelium in situ.
An in vitro culture system may be used to analyse interactions between T cells and epithelium. We have analysed two human buccal squamous epithelial cell lines: SqCC/Y1, derived from a buccal carcinoma, and SVpgC2a, an SV-transformed healthy buccal squamous epithelium. Under serum-free culture conditions, the cell lines resemble normal buccal squamous epithelium in situ in their expression of MHC class I, CD29 (beta1 integrin), CD40, CD44, CD54 (ICAM-1), CD58 (LFA-3), CD95 (Fas), and E-cadherin. Furthermore, when the SVpgC2a cell line was treated with T-cell derived cytokines, upregulation of CD40 expression was observed when the cells were treated with IL-4, whereas IFN-gamma caused upregulation in expression of CD40, CD54 and MHC class II. These buccal squamous epithelial cell lines, therefore, provide a good tool for analysing interactions between epithelium and T cells in vitro.
Farmer
I
I
Department of Oral Medicine, Leeds Dental Institute, UK.
Freysdottir
J
J
Dalghous
A M
AM
Fortune
F
F
D019013:CD40 Antigens / Q000032:analysis; D018968:CD58 Antigens / Q000032:analysis; D015820:Cadherins / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000032:analysis* / Q000235:genetics; D002460:Cell Line; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D016895:Culture Media, Serum-Free; D016207:Cytokines / Q000494:pharmacology; D004847:Epithelial Cells / Q000166:cytology / Q000473:pathology; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D015395:Histocompatibility Antigens Class I / Q000032:analysis; D006801:Humans; D018960:Hyaluronan Receptors / Q000032:analysis; D019012:Integrin beta1 / Q000032:analysis; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D007371:Interferon-gamma / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology; D009061:Mouth Mucosa / Q000166:cytology* / Q000473:pathology; D009062:Mouth Neoplasms / Q000473:pathology; D013539:Simian virus 40; D014407:Tumor Cells, Cultured; D015854:Up-Regulation; D019014:fas Receptor / Q000032:analysis
D019013:CD40 Antigens; D018968:CD58 Antigens; D015820:Cadherins; D015815:Cell Adhesion Molecules; D016895:Culture Media, Serum-Free; D016207:Cytokines; D015395:Histocompatibility Antigens Class I; D018960:Hyaluronan Receptors; D019012:Integrin beta1; D019014:fas Receptor; D018799:Intercellular Adhesion Molecule-1; D015847:Interleukin-4; D007371:Interferon-gamma