Bacillus intermedius ribonuclease as inhibitor of cell proliferation and membrane current.
The antiproliferative action of the guanine-specific ribonuclease secreted by Bacillus intermedius (binase) was studied in different chicken and mouse cell lines. The proliferation rate of chicken embryo fibroblasts, either normal or Rous sarcoma virus-transformed, was significantly reduced by binase treatment. Among mouse fibroblasts, v-ras-transformed NIH3T3 cells were sensitive to binase, whereas the growth of non-transformed, v-src-transformed or v-fms-transformed NIH3T3 cells was not affected. A 48 h treatment with binase inhibited the Ca2+-dependent K+ current of v-ras-transformed NIH3T3 cells but had no effect on this membrane current in non-transformed and in v-src- or v-fms-transformed NIH3T3 cells. Our results suggest that mammalian cells expressing the ras-oncogene are a potential target for the antiproliferative action of binase.
Ilinskaya
O
O
Department of Microbiology, Kazan State University, Kremlevskaya Street 18, 420008 Kazan, Russia.
Decker
K
K
Koschinski
A
A
Dreyer
F
F
Repp
H
H
D016475:3T3 Cells / Q000187:drug effects* / Q000502:physiology; D000818:Animals; D001407:Bacillus / Q000201:enzymology*; D002118:Calcium / Q000502:physiology; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects*; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002642:Chick Embryo; D004305:Dose-Response Relationship, Drug; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D051379:Mice; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000187:drug effects; D012260:Ribonucleases / Q000378:metabolism / Q000494:pharmacology*
D015221:Potassium Channels; D012260:Ribonucleases; D002118:Calcium