Role of soy diet and L-arginine in cyclosporin-A-induced osteopenia in rats.
Our previous studies show that chronic administration of L-arginine decreases cyclosporin-A-induced bone loss. The present study was designed to investigate whether a soy diet could prevent cyclosporin A-induced osteopenia and eventually improve the protective effect of L-arginine. Rats on soy diet were treated with cyclosporin-A, L-arginine, cyclosporin-A + L-arginine or saline. Control groups received a normal diet and the same pharmacological treatment. Our results show that a soy diet prevents osteopenia only in the spinal cord (+30%) and confirm the protective effect of L-arginine in cyclosporin-A-induced osteopenia in whole body, pelvis and spine of rats on a normal diet (+31%, +55%, +55%, respectively). Moreover these data show that the osteoprotective effect of L-arginine in the whole body, pelvis and spine improves in the case of soy diet (+60%, +72%, +89%, respectively). The results suggest that a soy diet exerts a positive effect in cyclosporin-A-induced osteopenia only in sites with high turn-over and improves the osteoprotective effect of L-arginine.
Clementi
G
G
Department of Experimental and Clinical Pharmacology, School of Medicine, University of Catania, Italy. mangano@tin.it
Fiore
C E
CE
Mangano
N G
NG
Cutuli
V M
VM
Pennisi
P
P
Caruso
A
A
Prato
A
A
Matera
M
M
Amico-Roxas
M
M
D000818:Animals; D001120:Arginine / Q000008:administration & dosage / Q000627:therapeutic use*; D001835:Body Weight / Q000187:drug effects; D015519:Bone Density / Q000187:drug effects; D001851:Bone Diseases, Metabolic / Q000097:blood / Q000139:chemically induced / Q000000981:diagnostic imaging / Q000517:prevention & control*; D002118:Calcium / Q000097:blood; D016572:Cyclosporine / Q000633:toxicity; D004032:Diet; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D007166:Immunosuppressive Agents / Q000633:toxicity; D007274:Injections, Intraperitoneal; D008297:Male; D010384:Pelvic Bones / Q000000981:diagnostic imaging / Q000187:drug effects / Q000378:metabolism; D011859:Radiography; D051381:Rats; D017207:Rats, Sprague-Dawley; D030262:Soybean Proteins / Q000008:administration & dosage*; D013131:Spine / Q000000981:diagnostic imaging / Q000187:drug effects / Q000378:metabolism
D007166:Immunosuppressive Agents; D030262:Soybean Proteins; D016572:Cyclosporine; D001120:Arginine; D002118:Calcium