Angiotensin II increases calponin expression in cultured rat vascular smooth muscle cells.
Angiotensin II (Ang II) action on vascular smooth muscle cells is not limited to contraction, but includes long term effects such as hypertrophy and hyperplasia. This implies a complex pattern of gene modulation, which remains largely unknown. We used the mRNA differential display method to screen rat aortic smooth muscle cells cultured with or without Ang II. We demonstrated that Ang II induces the expression of calponin, a 34-kD protein, which has been shown to regulate smooth muscle cell contraction and to be a marker of smooth muscle cell differentiation. We demonstrated this induction both at gene and protein level in vascular smooth muscle cells. Calponin mRNA was dose-dependently induced by Ang II, with an effect still evident at 5 x 10(-9)M, and it did not require active protein synthesis, since cycloheximide treatment did not suppress this induction. Calponin gene expression was maximal at 3 h, while protein expression was maximal at 8 h. Calponin expression was completely abolished by the AT1 receptor antagonist, losartan, at 1 x 10(-6)M. Our data demonstrate that Ang II increases calponin gene expression and protein level in rat aortic smooth muscle cells in vitro.
di Gioia
C R
CR
Istituto di Cardiologia, Università Cattolica, Rome, Italy.
van de Greef
W M
WM
Sperti
G
G
Castoldi
G
G
Todaro
N
N
Ierardi
C
C
Pieruzzi
F
F
Stella
A
A
D000704:Analysis of Variance; D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology; D001011:Aorta / Q000166:cytology / Q000187:drug effects; D002135:Calcium-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D002454:Cell Differentiation; D002478:Cells, Cultured; D004347:Drug Interactions; D015870:Gene Expression / Q000187:drug effects*; D019808:Losartan / Q000494:pharmacology; D008297:Male; D008840:Microfilament Proteins; D009131:Muscle, Smooth, Vascular / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D000096985:Calponins
D000804:Angiotensin II; D000959:Antihypertensive Agents; D002135:Calcium-Binding Proteins; D019808:Losartan; D008840:Microfilament Proteins; D012333:RNA, Messenger; D000096985:Calponins