Dorsal dermis development depends on a signal from the dorsal neural tube, which can be substituted by Wnt-1.
To investigate the origin and nature of the signals responsible for specification of the dermatomal lineage, excised axial organs in 2-day-old chick embryos were replaced by grafts of the dorsal neural tube, or the ventral neural tube plus the notochord, or aggregates of cells engineered to produce Sonic hedgehog (Shh), Noggin, BMP-2, Wnt-1, or Wnt-3a. By E10, grafts of the ventral neural tube plus notochord or of cells producing Shh led to differentiation of cartilage and muscles, and an impaired dermis derived from already segmented somites. In contrast, grafts of the dorsal neural tube, or of cells producing Wnt-1, triggered the formation of a feather-inducing dermis. These results show that the dermatome inducer is produced by the dorsal neural tube. The signal can be Wnt-1 itself, or can be mediated, or at least mimicked by Wnt-1.
Olivera-Martinez
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Equipe Biologie de la Différenciation Epithéliale, UMR Centre National de la Recherche Scientifique (CNRS) 5538, LEDAC, Institut Albert Bonniot, Université Joseph Fourier, Grenoble, France.
Thélu
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Teillet
M A
MA
Dhouailly
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D
D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000378:metabolism; D002356:Cartilage / Q000166:cytology; D002452:Cell Count; D002454:Cell Differentiation; D002460:Cell Line; D002642:Chick Embryo; D003587:Cytomegalovirus / Q000235:genetics; D020405:Dermis / Q000196:embryology*; D053823:Hedgehog Proteins; D008953:Models, Anatomic; D009132:Muscles / Q000166:cytology; D009432:Neural Crest / Q000196:embryology*; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000502:physiology*; D015398:Signal Transduction; D019170:Somites / Q000378:metabolism; D013997:Time Factors; D016378:Tissue Transplantation; D015534:Trans-Activators; D016212:Transforming Growth Factor beta; D051153:Wnt Proteins; D051155:Wnt1 Protein; D060508:Wnt3 Protein; D029961:Zebrafish Proteins
D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D053823:Hedgehog Proteins; D011506:Proteins; D011518:Proto-Oncogene Proteins; D015534:Trans-Activators; D016212:Transforming Growth Factor beta; D051153:Wnt Proteins; D051155:Wnt1 Protein; D060508:Wnt3 Protein; D029961:Zebrafish Proteins