title: HLA class I diversity in Kolla Amerindians.
abstract: Human leukocyte antigen (HLA) class I polymorphism was studied within a population of 70 unrelated Kolla Amerindians from the far northwest of Argentina close to the Bolivian border. The results indicate that the HLA-A, -B, and -C alleles typical of other Amerindian populations also predominate in the Kolla. These alleles belong to the following allele groups: HLA-A*02, *68, *31, *24, HLA-B*35, *15, *51, *39, *40, *48, and Cw*01, *03, *04, *07, *08, and *15. For the HLA-A locus, heterogeneity was seen for HLA-A*02 with A*0201, *0211, and *0222; and for A*68 with *68012 and *6817, the latter being a novel allele identified in this population. Analysis of HLA-B identified heterogeneity for all Amerindian allele groups except HLA-B*48, including the identification of the novel B*5113 allele. For HLA-C heterogeneity was identified within the Cw*07, *04, and *08 groups with Cw*0701/06, *0702, *04011, *0404, *0803, and *0809 identified. The most frequent "probable" haplotype found in this population was B*3505-Cw*04011. This study supports previous studies, which demonstrate increased diversity at HLA-B compared with HLA-A and -C. The polymorphism identified within the Kolla HLA-A, -B, and -C alleles supports the hypothesis that HLA evolution is subject to positive selection for diversity within the peptide binding site.
authors.affiliation: Anthony Nolan Research Institute, The Royal Free Hospital, London, United Kingdom.
authors.forename: A M
authors.initials: AM
authors.lastname: Little
authors.forename: I
authors.initials: I
authors.lastname: Scott
authors.forename: S
authors.initials: S
authors.lastname: Pesoa
authors.forename: S G
authors.initials: SG
authors.lastname: Marsh
authors.forename: R
authors.initials: R
authors.lastname: Argüello
authors.forename: S T
authors.initials: ST
authors.lastname: Cox
authors.forename: D
authors.initials: D
authors.lastname: Ramon
authors.forename: C
authors.initials: C
authors.lastname: Vullo
authors.forename: J A
authors.initials: JA
authors.lastname: Madrigal
mesh_terms: D000483:Alleles; D001118:Argentina; D005260:Female; D006680:HLA Antigens / Q000235:genetics*; D015234:HLA-A Antigens / Q000235:genetics; D015235:HLA-B Antigens / Q000235:genetics; D015236:HLA-C Antigens / Q000235:genetics; D006239:Haplotypes / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006650:Histocompatibility Testing / Q000379:methods / Q000592:standards; D006801:Humans; D007199:Indians, South American / Q000235:genetics*; D008297:Male; D009690:Nucleic Acid Conformation; D015345:Oligonucleotide Probes / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic / Q000276:immunology; D012016:Reference Values; D017422:Sequence Analysis, DNA
chemical_list: D006680:HLA Antigens; D015234:HLA-A Antigens; D015235:HLA-B Antigens; D015236:HLA-C Antigens; D015395:Histocompatibility Antigens Class I; D015345:Oligonucleotide Probes