title: Rubella virus E2 signal peptide is required for perinuclear localization of capsid protein and virus assembly.
abstract: The rubella virus (RV) structural proteins capsid, E2, and E1 are synthesized as a polyprotein precursor. The signal peptide that initiates translocation of E2 into the lumen of the endoplasmic reticulum remains attached to the carboxy terminus of the capsid protein after cleavage by signal peptidase. Among togaviruses, this feature is unique to RV. The E2 signal peptide has previously been shown to function as a membrane anchor for the capsid protein. In the present study, we demonstrate that this domain is required for RV glycoprotein-dependent localization of the capsid protein to the juxtanuclear region and subsequent virus assembly at the Golgi complex.
authors.affiliation: Department of Cell Biology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada.
authors.forename: L M
authors.initials: LM
authors.lastname: Law
authors.forename: R
authors.initials: R
authors.lastname: Duncan
authors.forename: A
authors.initials: A
authors.lastname: Esmaili
authors.forename: H L
authors.initials: HL
authors.lastname: Nakhasi
authors.forename: T C
authors.initials: TC
authors.lastname: Hobman
mesh_terms: D000818:Animals; D019556:COS Cells; D002213:Capsid / Q000235:genetics / Q000378:metabolism*; D021382:Protein Sorting Signals / Q000235:genetics / Q000502:physiology*; D021381:Protein Transport; D012412:Rubella virus / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014162:Transfection; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*; D019065:Virus Assembly
chemical_list: D021382:Protein Sorting Signals; D014759:Viral Envelope Proteins; C061827:E2 envelope protein, Rubella virus