title: Coffee enhances the development of 7,12-dimethylbenz[a]anthracene (DMBA)-induced hamster buccal pouch carcinomas.
abstract: In this study, we examined the effect of roasted coffee extract on 7,12-dimethylbenz[a]anthracene (DMBA)-induced buccal pouch carcinogenesis in male Syrian hamsters using lipid peroxidation, reduced glutathione (GSH) and glutathione peroxidase (GPx) activity as biomarkers of chemoprevention. Forty male hamsters were divided into four groups of 10 animals. The right buccal pouches of the animals in Group 1 was painted with a 0.5% solution of DMBA in liquid paraffin three times a week. The animals in Group 2 painted with DMBA as in Group 1, received in addition 2 ml of 8% black coffee extract intragastrically three times a week on days alternate to DMBA application. Group 3 animals received coffee extract as in Group 2. Animals in Group 4 received neither DMBA nor coffee extract and served as control. The hamsters were sacrificed after an experimental period of 14 weeks. Biochemical measurements were carried out on tumour and normal pouch tissues. Administration of roasted coffee extract had no preventive effect on DMBA-induced oral cancer as revealed by the higher mean tumour volume and tumour burden compared to animals painted with DMBA alone. Diminished lipid peroxidation in the oral tumour tissue was accompanied by a significant increase in the levels of GSH and GPx.
  
  
  CONCLUSIONS
  The results of the present study suggest that coffee exerts a tumour enhancing effect when administered during DMBA-induced hamster buccal pouch carcinogenesis.
authors.affiliation: Department of Biochemistry, Faculty of Science, Annamalainagar-608 002, Tamil Nadu, India.
authors.forename: M
authors.initials: M
authors.lastname: Saroja
authors.forename: S
authors.initials: S
authors.lastname: Balasenthil
authors.forename: C R
authors.initials: CR
authors.lastname: Ramachandran
authors.forename: S
authors.initials: S
authors.lastname: Nagini
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002273:Carcinogens; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D003069:Coffee / Q000009:adverse effects*; D006224:Cricetinae; D004347:Drug Interactions; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008647:Mesocricetus; D009061:Mouth Mucosa / Q000187:drug effects; D009062:Mouth Neoplasms / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
chemical_list: D000975:Antioxidants; D002273:Carcinogens; D003069:Coffee; D017392:Thiobarbituric Acid Reactive Substances; D015127:9,10-Dimethyl-1,2-benzanthracene; D005979:Glutathione Peroxidase; D005978:Glutathione