Short-range and long-range guidance by slit and its Robo receptors. Robo and Robo2 play distinct roles in midline guidance.
Previous studies showed that Roundabout (Robo) in Drosophila is a repulsive axon guidance receptor that binds to Slit, a repellent secreted by midline glia. In robo mutants, growth cones cross and recross the midline, while, in slit mutants, growth cones enter the midline but fail to leave it. This difference suggests that Slit must have more than one receptor controlling midline guidance. In the absence of Robo, some other Slit receptor ensures that growth cones do not stay at the midline, even though they cross and recross it. Here we show that the Drosophila genome encodes three Robo receptors and that Robo and Robo2 have distinct functions, which together control repulsive axon guidance at the midline. The robo,robo2 double mutant is largely identical to slit.
Simpson
J H
JH
Howard Hughes Medical Institute, Division of Neurobiology, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA.
Kidd
T
T
Bland
K S
KS
Goodman
C S
CS
D000818:Animals; D001369:Axons / Q000378:metabolism; D019556:COS Cells; D002460:Cell Line; D002490:Central Nervous System / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D003001:Cloning, Molecular; D019281:Dimerization; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D004330:Drosophila; D029721:Drosophila Proteins; D015870:Gene Expression / Q000187:drug effects; D020439:Growth Cones / Q000473:pathology; D006801:Humans; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D008024:Ligands; D008565:Membrane Proteins / Q000378:metabolism / Q000494:pharmacology; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D012333:RNA, Messenger / Q000096:biosynthesis; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism*; D000097905:Roundabout Proteins
D029721:Drosophila Proteins; D019476:Insect Proteins; D008024:Ligands; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; D000097905:Roundabout Proteins; C498890:ROBO2 protein, human; C421243:Robo2 protein, Drosophila; C110665:comm protein, Drosophila; C421244:robo3 protein, Drosophila; C066819:sli protein, Drosophila