The delivery of protein into living cells by use of membrane fusible erythrocyte ghosts.
We examined the effects of pretreatment of monkey kidney normal cells (CV-1) and human cervical tumor cells (HeLa) with membrane fusible erythrocyte ghosts (MFEG) encapsulating superoxide dismutase (SOD-MFEG) on adriamycin (ADM) cytotoxicity. The decrease in CV-1 cells with ADM was inhibited markedly, and the initiation of decrease in HeLa cells was delayed for 2 days by the pretreatment with SOD-MFEG, indicating that the delivered SOD held the activity in the cells.
Kogure
K
K
Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
Itoh
T
T
Okuda
O
O
Hayashi
K
K
Ueno
M
M
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage; D002460:Cell Line; D004317:Doxorubicin / Q000008:administration & dosage; D016503:Drug Delivery Systems; D004910:Erythrocyte Membrane / Q000378:metabolism*; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D000882:Haplorhini; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D011506:Proteins / Q000008:administration & dosage*; D013482:Superoxide Dismutase / Q000008:administration & dosage
D000903:Antibiotics, Antineoplastic; D005456:Fluorescent Dyes; D011506:Proteins; D004317:Doxorubicin; D013482:Superoxide Dismutase; D016650:Fluorescein-5-isothiocyanate