title: Generation of IgM anti-platelet autoantibody in dengue patients.
abstract: Dengue virus infection causes a wide range of diseases from dengue fever to life-threatening dengue hemorrhagic fever and dengue shock syndrome (DHF/DSS). The mechanisms involved in DHF/DSS pathogenesis remain unclear. Patient sera collected from an outbreak in southern Taiwan from November 1998 to January 1999 were studied. The presence of antibodies which cross-reacted with platelets could be detected in patient sera, and the isotype of these autoantibodies was IgM. The anti-platelet IgM levels were higher in DHF/DSS than in dengue fever patient sera in disease acute phase. These autoantibodies were still detectable in convalescent stage (1-3 weeks after acute phase) and even eight to nine months after illness. The platelet binding activity was not observed in other virus-infected patient sera tested. Further investigation showed that dengue patient sera caused platelet lysis in the presence of complement. The platelet cytotoxicity induced by DHF/DSS patient sera was higher than that by dengue fever sera. Dengue patient sera also inhibited platelet aggregation which, however, appeared to be not related to DHF/DSS development.
authors.affiliation: Department of Microbiology and Immunology, National Cheng Kung University Medical College, Tainan, Taiwan.
authors.forename: C F
authors.initials: CF
authors.lastname: Lin
authors.forename: H Y
authors.initials: HY
authors.lastname: Lei
authors.forename: C C
authors.initials: CC
authors.lastname: Liu
authors.forename: H S
authors.initials: HS
authors.lastname: Liu
authors.forename: T M
authors.initials: TM
authors.lastname: Yeh
authors.forename: S T
authors.initials: ST
authors.lastname: Wang
authors.forename: T I
authors.initials: TI
authors.lastname: Yang
authors.forename: F C
authors.initials: FC
authors.lastname: Sheu
authors.forename: C F
authors.initials: CF
authors.lastname: Kuo
authors.forename: Y S
authors.initials: YS
authors.lastname: Lin
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D001323:Autoantibodies / Q000097:blood*; D001792:Blood Platelets / Q000276:immunology*; D002648:Child; D003289:Convalescence; D003715:Dengue / Q000097:blood / Q000453:epidemiology / Q000276:immunology*; D004196:Disease Outbreaks; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood*; D008297:Male; D008875:Middle Aged; D013624:Taiwan / Q000453:epidemiology
chemical_list: D001323:Autoantibodies; D007075:Immunoglobulin M