Interleukin-10 relieves the inhibitory effects of interferon-gamma on normal human lung fibroblasts.
Interleukin-10 (IL-10) is an important cytokine that suppresses the production of cytokines and chemokines by immune cells. IL-10 has been suggested to be involved in chronic inflammatory responses including the remodeling process in the lung. We investigated the effects of IL-10 on proliferation, extracellular matrix and cytokine production in normal human lung fibroblasts (NHLF). Human IL-10 (hIL-10) complementary DNA (cDNA) was transfected into NHLF using an adenoviral vector. No significant changes were observed in proliferation, fibronectin or procollagen type I production in the NHLF transfected with hIL-10 cDNA. Interferon (IFN)-gamma significantly inhibited cell proliferation and extracellular matrix production in a dose-dependent manner. Transfection of hIL-10 cDNA significantly relieved the suppressive effects of IFN-gamma in NHLF. Transforming growth factor (TGF)-beta production was not significantly affected by either transfection of hIL-10 cDNA or the addition of IFN-gamma. The relief of the suppressive function of IFN-gamma by IL-10 suggested that IL-10 is indirectly involved in the remodeling process in the lung interstitium.
Hashimoto
T
T
Department of Pathology, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259-1193, Japan.
Nakamura
M
M
Oshika
Y
Y
Tsuchida
T
T
Yamazaki
H
H
Kijima
H
H
Ueyama
Y
Y
Minoguchi
K
K
Adachi
M
M
Ota
H
H
D000256:Adenoviridae / Q000235:genetics; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D018076:DNA, Complementary; D004305:Dose-Response Relationship, Drug; D005109:Extracellular Matrix / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D008168:Lung / Q000166:cytology*; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000187:drug effects
D018076:DNA, Complementary; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D007371:Interferon-gamma