title: The renal nerve is required for regulation of proximal tubule transport by intraluminally produced ANG II.
abstract: The proximal tubule synthesizes and luminally secretes high levels of angiotensin II, which modulate proximal tubule transport independently of systemic angiotensin II. The purpose of this in vivo microperfusion study is to examine whether the renal nerves modulate the effect of intraluminal angiotensin II on proximal tubule transport. The decrement in volume reabsorption after addition of 10(-4) M luminal enalaprilat is a measure of the role of luminal angiotensin II on transport. Acute denervation decreased volume reabsorption (2.97 +/- 0.14 vs. 1.30 +/- 0.21 nl. mm(-1). min(-1), P < 0.001). Although luminal 10(-4) M enalaprilat decreased volume reabsorption in controls (2.97 +/- 0.14 vs. 1.61 +/- 0.26 nl. mm(-1). min(-1), P < 0.001), it did not after acute denervation (1.30 +/- 0.21 vs. 1.55 +/- 0.19 nl. mm(-1). min(-1)). After chronic denervation, volume reabsorption was unchanged from sham controls (2.26 +/- 0.28 vs. 2.70 +/- 0.19 nl. mm(-1). min(-1)). Addition of luminal 10(-4) M enalaprilat decreased volume reabsorption in sham control (2.70 +/- 0.19 vs. 1.60 +/- 0.10 nl. mm(-1). min(-1), P < 0.05) but not with chronic denervation (2.26 +/- 0.28 vs. 2.07 +/- 0.20 nl. mm(-1). min(-1)). Addition of 10(-8) M angiotensin II to the lumen does not affect transport due to the presence of luminal angiotensin II. However, addition of 10(-8) M angiotensin II to the tubular lumen increased the volume reabsorption after both acute (1.30 +/- 0.21 vs. 2.67 +/- 0.18 nl. mm(-1). min(-1), P < 0.05) and chronic denervation (2.26 +/- 0.28 vs. 3.57 +/- 0.44 nl. mm(-1). min(-1), P < 0.01). These data indicate that renal denervation abolished the luminal enalaprilat-sensitive component of proximal tubule transport, which is consistent with the renal nerves playing a role in the modulation of the intraluminal angiotensin II mediated component of proximal tubule transport.
authors.affiliation: Department of Pediatrics, The University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75235-9063, USA. albert.aquan@utsouthwestern.edu
authors.forename: A
authors.initials: A
authors.lastname: Quan
authors.forename: M
authors.initials: M
authors.lastname: Baum
mesh_terms: D000042:Absorption / Q000187:drug effects / Q000502:physiology; D000804:Angiotensin II / Q000494:pharmacology / Q000502:physiology*; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport / Q000502:physiology; D003714:Denervation; D015773:Enalaprilat / Q000494:pharmacology; D007668:Kidney / Q000294:innervation*; D007687:Kidney Tubules, Proximal / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013564:Sympathetic Nervous System / Q000502:physiology*
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000804:Angiotensin II; D015773:Enalaprilat