Antigen-specific T cell tolerance down-regulates mast cell responses in vivo.
Fel d I is the major cat allergen that induces asthma and allergic rhinitis in humans. To investigate the mechanism of allergic responses to this allergen, a mouse model was developed. Mice sensitized to chain 1 of Fel d I exhibited T cell responses, B cell responses, and mast cell responses when challenged with the protein. Subcutaneous injections of peptides containing the dominant T cell epitopes of the allergen induced T cell tolerance in presensitized mice. When challenged with the allergen intratracheally, these tolerized mice produced a decreased amount of histamine in vivo. The decrease in histamine release was not solely dependent on the reduction of allergen-specific IgE. These data show that mast cell activity in mice with an ongoing sensitivity to allergen can be regulated through peptide-induced T cell tolerance.
Treter
S
S
ImmuLogic Pharmaceutical Corporation, 610 Lincoln Street, Waltham, Massachusetts 02154, USA.
Luqman
M
M
D000485:Allergens / Q000008:administration & dosage / Q000276:immunology; D000818:Animals; D002478:Cells, Cultured; D004140:Dinitrophenols / Q000008:administration & dosage / Q000276:immunology; D015536:Down-Regulation / Q000276:immunology*; D018984:Epitopes, T-Lymphocyte / Q000008:administration & dosage / Q000276:immunology*; D005260:Female; D006023:Glycoproteins / Q000008:administration & dosage / Q000276:immunology; D006241:Haptens / Q000008:administration & dosage / Q000276:immunology; D006636:Histamine Release / Q000276:immunology; D006969:Hypersensitivity, Immediate / Q000276:immunology; D007108:Immune Tolerance / Q000276:immunology*; D007116:Immunization, Passive; D007073:Immunoglobulin E / Q000008:administration & dosage / Q000096:biosynthesis; D007275:Injections, Intravenous; D007279:Injections, Subcutaneous; D007442:Intubation, Intratracheal; D008407:Mast Cells / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D010446:Peptide Fragments / Q000008:administration & dosage / Q000276:immunology; D012709:Serum Albumin / Q000008:administration & dosage / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000485:Allergens; D004140:Dinitrophenols; D018984:Epitopes, T-Lymphocyte; D006023:Glycoproteins; D006241:Haptens; D010446:Peptide Fragments; D012709:Serum Albumin; C058064:dinitrophenyl-human serum albumin conjugate; D007073:Immunoglobulin E; C059296:Fel d 1 protein, Felis domesticus