Chemokine production by peripheral blood mononuclear cells in elderly subjects.
The function of chemokines in promoting and modulating leukocyte migration is essential for a prompt and efficacious inflammatory response and in host defence against infections. In order to investigate whether this important aspect of immunological response is influenced by ageing, we evaluated the basal levels as well as the ability of peripheral blood mononuclear cells from young and healthy elderly subjects to produce chemokines (IL-8, MCP-1, MIP-Ialpha, RANTES) in response to stimulation with anti-CD3 monoclonal antibody and lipopolysaccharide (LPS), a gram negative bacterial endotoxin. Our main findings are a spontaneous chemokine production; a 20% decrease of proliferative response to anti-CD3 monoclonal antibody accompanied by an age related increase of MIP-Ialpha and RANTES production and by a general increase of all chemokine production compared to unstimulated conditions; a proliferative defect of monocytes to LPS challenge associated with an increase of chemokine production compared to basal conditions with a progressive age-related increase of MIP-lalpha. In conclusion, this study suggests that chemokines could have a compensatory role in balancing the impaired mechanisms involved in 'specific' immune response during ageing. The successful activation of this strategy could contribute to the good performance of immune system so maintaining healthy status in elderly.
Pulsatelli
L
L
Laboratorio di Immunologia e Genetica, Dipartimento di Medicina Interna e Gastroenterologia, Università degli Studi di, Istituto di Ricerca Codivilla-Putti, IOR, Via di Barbiano 1/10, 40136, Bologna, Italy.
Meliconi
R
R
Mazzetti
I
I
Dolzani
P
P
Meneghetti
A
A
Neri
S
S
Silvestri
T
T
Ravaglia
G
G
Forti
P
P
Facchini
A
A
Mariani
E
E
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000097:blood*; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D017252:CD3 Complex / Q000276:immunology; D002455:Cell Division / Q000187:drug effects; D018925:Chemokines / Q000096:biosynthesis*; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D009000:Monocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D010641:Phenotype
D000911:Antibodies, Monoclonal; D017252:CD3 Complex; D018925:Chemokines; D008070:Lipopolysaccharides; D003208:Concanavalin A