Intravital demonstration of modulation of T lymphocyte migration by CINC/gro in rat Peyer's patches.
OBJECTIVE
Cytokine-induced neutrophil chemoattractant (CINC/gro), a member of interleukin-8 family, was found as a potent chemotactic factor for rat neutrophils. Although several chemokines have been shown to be potent regulators of T cell chemotaxis in vitro, the potential role of chemokines in T-cell migration in gut-associated lymphoid tissues has not been investigated in vivo. In the present study, the effects of CINC/gro on T-lymphocyte migration were examined in rat Peyer's patches.


METHODS
T lymphocytes collected from intestinal lymph of rats were fluorescence-labeled and injected into the jugular vein. Peyer's patches of the recipient rats were observed with intravital fluorescence microscopy and the effects of CINC/gro infusion was investigated. Lymphocyte flux in mesenteric collecting lymphatics was also observed.


RESULTS
In vivo infusion of CINC/gro significantly attenuated the initial lymphocyte interaction with postcapillary venules of Peyer's patches. However, once these lymphocytes adhered to venules, CINC/gro treatment significantly accelerated the transendothelial migration of T lymphocytes and they also significantly increased their subsequent flux in collecting lymphatics.


CONCLUSIONS
There is a possibility that CINC/gro could modulate the characteristics of T lymphocyte homing in the inflammatory sites of gut.
Fujimori
H
H
Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan.
Miura
S
S
Koseki
S
S
Hokari
R
R
Tsuzuki
Y
Y
Komoto
S
S
Hara
Y
Y
Suzuki
H
H
Serizawa
H
H
Ishii
H
H
D000818:Animals; D002448:Cell Adhesion; D002465:Cell Movement; D054360:Chemokine CXCL1; D019743:Chemokines, CXC; D002630:Chemotactic Factors / Q000494:pharmacology*; D002633:Chemotaxis; D006131:Growth Inhibitors / Q000494:pharmacology*; D006133:Growth Substances / Q000494:pharmacology*; D007249:Inflammation; D036341:Intercellular Signaling Peptides and Proteins; D007421:Intestine, Small / Q000276:immunology* / Q000473:pathology; D008208:Lymphatic System; D008297:Male; D010581:Peyer's Patches / Q000276:immunology*; D051381:Rats; D017208:Rats, Wistar; D013601:T-Lymphocytes / Q000502:physiology*
D054360:Chemokine CXCL1; D019743:Chemokines, CXC; D002630:Chemotactic Factors; C513297:Cxcl1 protein, rat; D006131:Growth Inhibitors; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins