Neurotoxicity of 2-bromopropane and 1-bromopropane, alternative solvents for chlorofluorocarbons.
To clarify the neurotoxicity of 2-bromopropane (2-BP) in comparison with 1-bromopropane (1-BP), 36 Wistar strain male rats were divided into 4 groups of 9 and exposed daily to 100-ppm 2-BP, 1000-ppm 2-BP, 1000-ppm 1-BP, or fresh air for 8 h a day. Exposure to 1000 ppm of 1-BP was discontinued after 5 or 7 weeks' exposure because of the unexpected appearance of incomplete hindlimb paralysis followed by serious emaciation. The other groups were sacrificed at the end of 12 weeks' exposure. Exposure to 1000 ppm of 2-BP resulted in significant decreases in body weight and motor nerve conduction velocity (MCV) and elongation in distal latency (DL). A ball-like enlargement of myelin sheaths was observed. Significant reductions in the number of erythrocytes, platelets, and leukocytes, testicular germ cell loss, and seminiferous atrophy were also observed in this group, but not in 100-ppm 2-BP group. Exposure to 1000 ppm of 1-BP for 5 or 7 weeks caused a significant decrease in body weight and MCV and elongation in DL. Linearly arranged ovoid- or bubble-like debris of the axons and myelin sheaths in the teased tibial nerves and axonal swelling in gracilis nucleus were found in this group. No significant changes in hematological indices or histopathological findings of the testis were found in this group. In conclusion, 2-BP is neurotoxic to the peripheral nerves in addition to its toxic effects on the reproductive and hematopoietic systems at 1000 ppm. No noticeable changes were found in the rats exposed to 100 ppm of 2-BP. 1-BP is a potent neurotoxicant at 1000 ppm for 5 or 7 weeks, while testicular and hematopoietic toxicity was not found.
Yu
X
X
Department of Hygiene, Nagoya University School of Medicine, Nagoya, Japan.
Ichihara
G
G
Kitoh
J
J
Xie
Z
Z
Shibata
E
E
Kamijima
M
M
Takeuchi
Y
Y
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D017402:Chlorofluorocarbons; D004822:Epididymis / Q000187:drug effects / Q000473:pathology; D004906:Erythrocyte Count; D006400:Hematocrit; D006454:Hemoglobins / Q000032:analysis; D006651:Histocytochemistry; D006842:Hydrocarbons, Brominated / Q000633:toxicity*; D008297:Male; D009186:Myelin Sheath / Q000187:drug effects / Q000473:pathology; D009431:Neural Conduction / Q000187:drug effects* / Q000502:physiology; D010976:Platelet Count; D051381:Rats; D017208:Rats, Wistar; D012997:Solvents / Q000633:toxicity*; D013737:Testis / Q000187:drug effects / Q000473:pathology; D013979:Tibial Nerve / Q000187:drug effects / Q000473:pathology
D017402:Chlorofluorocarbons; D006454:Hemoglobins; D006842:Hydrocarbons, Brominated; D012997:Solvents; C103736:2-bromopropane; C118559:1-bromopropane