Spreading depolarization waves triggered by vagal stimulation in the embryonic chick brain: optical evidence for intercellular communication in the developing central nervous system.
Throughout experiments on multiple-site voltage-sensitive dye recordings of neural activity in embryonic chick brain preparations, we have found a novel type of depolarization waves which spread widely from the brainstem to the whole brain region at a rapid rate (mm/s). This depolarization wave was triggered by glutamate-mediated postsynaptic potentials and was especially correlated to N-methyl-D-aspartate receptor function. Evidence that the spreading depolarization wave is eliminated by octanol or 18beta-glycyrrhetinic acid suggests that the depolarization wave depends on functions of gap junctions. The profile obtained with Ca(2+)-imaging experiments also suggests that the propagation of the depolarization wave is accompanied by a calcium wave. These results provide new evidence for intercellular functional communication between neural cells in the vertebrate central nervous system during embryonic development.
Momose-Sato
Y
Y
Department of Physiology, Tokyo Medical and Dental University Graduate School and facility of Medicine, Bunkyo-ku, 113-8519, Tokyo, Japan. yoko.phy2@med.tmd.ac.jp
Sato
K
K
Mochida
H
H
Yazawa
I
I
Sasaki
S
S
Kamino
K
K
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000196:embryology / Q000502:physiology*; D001931:Brain Mapping; D001933:Brain Stem / Q000187:drug effects / Q000196:embryology / Q000502:physiology*; D002118:Calcium / Q000378:metabolism; D002450:Cell Communication / Q000187:drug effects / Q000502:physiology*; D002642:Chick Embryo; D004396:Coloring Agents; D004558:Electric Stimulation; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects / Q000502:physiology; D017629:Gap Junctions / Q000187:drug effects / Q000378:metabolism; D006034:Glycyrrhetinic Acid / Q000494:pharmacology; D066298:In Vitro Techniques; D008274:Magnesium / Q000378:metabolism / Q000494:pharmacology; D000442:Octanols / Q000494:pharmacology; D055095:Optics and Photonics; D011930:Reaction Time / Q000187:drug effects / Q000502:physiology; D012684:Sensory Thresholds / Q000502:physiology; D013116:Spinal Cord / Q000187:drug effects / Q000502:physiology*; D014630:Vagus Nerve / Q000196:embryology / Q000502:physiology*
D004396:Coloring Agents; D018691:Excitatory Amino Acid Antagonists; D000442:Octanols; D008274:Magnesium; D006034:Glycyrrhetinic Acid; D002118:Calcium