title: Characterization of an unusual variant mRNA of human lysosomal alpha-mannosidase.
abstract: Lysosomal alpha-mannosidase (EC 3.2.1.24) is an exoglycosidase in the glycoprotein degradation pathway and is encoded by a 3.0 kb cDNA. A 2.3 kb cDNA from a minor species of HeLa cell mRNA was discovered by RT-PCR cloning. Southern blotting and PCR analysis of the HeLa cell genomic DNA showed that the 2.3 kb message was encoded by the lysosomal alpha-mannosidase gene. Sequence comparison of the cDNA with the corresponding genomic DNA indicated that the 2.3 kb message was generated by an unusual intra-exonic joining event.
authors.affiliation: Laboratory of Pathology and Center for Comparative Medical Genetics, School of Veterinary Medicine, University of Pennsylvania, Philadelphia 19104, USA.
authors.forename: H
authors.initials: H
authors.lastname: Sun
authors.forename: J H
authors.initials: JH
authors.lastname: Wolfe
mesh_terms: D017398:Alternative Splicing; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons; D006367:HeLa Cells; D006801:Humans; D008247:Lysosomes / Q000201:enzymology*; D008361:Mannosidases / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D043323:alpha-Mannosidase
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D008361:Mannosidases; D043323:alpha-Mannosidase