Systemic inflammation increases shear stress-induced platelet plug formation measured by the PFA-100.
The PFA-100 measures platelet plug formation under shear stress and is strongly dependent on von Willebrand Factor (VWF) levels in plasma. We therefore hypothesized that elevated VWF levels, possibly as a result of acute inflammation, adversely influence PFA-100 results. Healthy volunteers received either 2 ng/kg endotoxin or placebo in a randomized controlled trial. Four hours after endotoxin (but not placebo) infusion VWF levels increased by 85%, collagen epinephrine-induced closure time (CT) decreased by 47% and collagen ADP-CT decreased by 38% (P < 0.0001) respectively. In conclusion, systemic inflammation has a major impact on the results obtained by PFA-100 and may confound interpretation of platelet function.
Homoncik
M
M
Department of Clinical Pharmacology-TARGET, Vienna University Hospital School of Medicine, Vienna, Austria.
Blann
A D
AD
Hollenstein
U
U
Pernerstorfer
T
T
Eichler
H G
HG
Jilma
B
B
D000208:Acute Disease; D000244:Adenosine Diphosphate; D000328:Adult; D004837:Epinephrine; D006801:Humans; D007249:Inflammation / Q000097:blood*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D010976:Platelet Count; D010979:Platelet Function Tests; D012680:Sensitivity and Specificity; D014841:von Willebrand Factor / Q000502:physiology*
D008070:Lipopolysaccharides; D014841:von Willebrand Factor; D000244:Adenosine Diphosphate; D004837:Epinephrine