Dynamic regulation of the tryptophan operon: a modeling study and comparison with experimental data.
A mathematical model for regulation of the tryptophan operon is presented. This model takes into account repression, feedback enzyme inhibition, and transcriptional attenuation. Special attention is given to model parameter estimation based on experimental data. The model's system of delay differential equations is numerically solved, and the results are compared with experimental data on the temporal evolution of enzyme activity in cultures of Escherichia coli after a nutritional shift (minimal + tryptophan medium to minimal medium). Good agreement is obtained between the numeric simulations and the experimental results for wild-type E. coli, as well as for two different mutant strains.
Santillan
M
M
Department of Physiology, McGill University, McIntyre Medical Sciences Building, 3655 Drummond Street, Montreal, QC, Canada H3G 1Y6.
Mackey
M C
MC
D000878:Anthranilate Synthase / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics*; D003198:Computer Simulation; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D008432:Mathematical Computing; D008957:Models, Genetic; D009876:Operon; D012097:Repressor Proteins / Q000235:genetics*; D012270:Ribosomes / Q000378:metabolism; D014158:Transcription, Genetic; D014364:Tryptophan / Q000378:metabolism*; D029968:Escherichia coli Proteins
D000878:Anthranilate Synthase; D001426:Bacterial Proteins; D012097:Repressor Proteins; D014364:Tryptophan; C027190:TRPR protein, E coli; D029968:Escherichia coli Proteins