title: Identification of an alternatively spliced variant of human CD86 mRNA.
abstract: CD86 is a costimulatory molecule constitutively expressed by human antigen presenting cells which interacts with CD28 and CTLA-4 expressed by T cells. We have recently reported the identification of an alternatively spliced CD86 mRNA variant (CD86deltaTM) characterized by the deletion of exon 6 which encodes for the transmembrane domain. We report here the identification of an alternatively spliced variant (called CD86deltaEC) expressed by nonstimulated human monocytes and characterized by the deletion of exons 4 and 5 which encode for the extracellular V-like and C-like domains, respectively. The activation of monocytes by IFNgamma (i) induces the preferential expression of the transcript encoding for the membrane form and (ii) increases the expression of CD86 and of the accessory molecules CD40, CD49d and CD54. These results suggest that resting human monocytes may constitutively express different forms of CD86 which can then influence the activation of T cells.
authors.affiliation: Centre d'Immunologie Pierre Fabre, Saint Julien en Genevois, France.
authors.forename: G
authors.initials: G
authors.lastname: Magistrelli
authors.forename: G
authors.initials: G
authors.lastname: Caron
authors.forename: J F
authors.initials: JF
authors.lastname: Gauchat
authors.forename: P
authors.initials: P
authors.lastname: Jeannin
authors.forename: J Y
authors.initials: JY
authors.lastname: Bonnefoy
authors.forename: Y
authors.initials: Y
authors.lastname: Delneste
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D015703:Antigens, CD / Q000235:genetics*; D051940:B7-2 Antigen; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009000:Monocytes / Q000166:cytology / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D015703:Antigens, CD; D051940:B7-2 Antigen; C497964:CD86 protein, human; D018076:DNA, Complementary; D008562:Membrane Glycoproteins; D012333:RNA, Messenger