title: Effects of enamel matrix derivative on gene expression of primary osteoblasts.
abstract: OBJECTIVE
  The aim of this study was to investigate the effects of enamel matrix derivative (EMD) on gene expression of collagen alpha1 (I), osteocalcin, prostaglandin G/H synthase 2 (PGHS-2), interleukin-6, and insulin-like growth factor I in primary mouse osteoblasts.
  
  
  METHODS
  Primary osteoblasts were digested from 6- to 8-day-old mouse calvaria. Cells were divided into 4 groups and cultured for 24, 48, and 72 hours with a serum-free modified Eagle medium as negative control, modified Eagle medium with 25 microg/mL EMD, modified Eagle medium with 100 microg/mL EMD, and modified Eagle medium plus 10% fetal bovine serum as positive control. Gene expression was determined by Northern blot and reverse transcription polymerase chain reaction analysis.
  
  
  RESULTS
  EMD enhanced collagen I, interleukin-6, and PGHS-2 expression and did not stimulate the expression of osteocalcin and IGF-I.
  
  
  CONCLUSIONS
  These results indicate that EMD might regulate certain gene expression during periodontal tissue regeneration.
authors.affiliation: Department of Endodontology, School of Dental Medicine, University of Connecticut Health Center, Farmington, USA.
authors.forename: J
authors.initials: J
authors.lastname: Jiang
authors.forename: A F
authors.initials: AF
authors.lastname: Fouad
authors.forename: K E
authors.initials: KE
authors.lastname: Safavi
authors.forename: L S
authors.initials: LS
authors.lastname: Spångberg
authors.forename: Q
authors.initials: Q
authors.lastname: Zhu
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D003746:Dental Enamel Proteins / Q000494:pharmacology*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D015675:Osteocalcin / Q000096:biosynthesis; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D003746:Dental Enamel Proteins; D015850:Interleukin-6; C109069:enamel matrix proteins; D015675:Osteocalcin; D007334:Insulin-Like Growth Factor I; D003094:Collagen; D011451:Prostaglandin-Endoperoxide Synthases