Modulation of exogenous and endogenous levels of thioredoxin in human skin fibroblasts prevents DNA damaging effect of ultraviolet A radiation.
Thioredoxin (Trx) plays important biological roles both intra- and extracellularly via thiol redox control. We have previously demonstrated that Trx exhibited protective effects against UVA cytotoxicity in human skin fibroblasts. As an extension of the latter investigation, the present work is aimed at assessing ability of Trx to maintain genomic integrity in human skin fibroblasts upon exposure to UVA radiation. Indeed, UVA (320--380 nm) is mutagenic and induces genomic damage to skin cells. The alkaline comet assay was used in association with DNA repair enzyme including formamido pyrimidine glycosylase (Fpg) and endonuclease III (endo III) to estimate the amount of modified bases together with the level of strand breaks and alkali-labile sites. The HPLC-EC assay was applied to assess 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) levels and to permit the calibration of comet assay as previously described. We reported that overexpression of human Trx (transient transfection) as well as exogenous human recombinant Trx added to the culture medium, decreased the level of DNA damage in UVA irradiated cells. Interestingly, transfection appeared to prevent UVA-induced 8-oxodGuo (3.06 au per Joules.cm(-2) compared to 4.94 au per Joules.cm(-2) for nontransfected cells). Moreover, Trx accumulates into nuclei in transfected cells. This finding supports the notion that Trx is important for the maintenance of the integrity of genetic information. This work demonstrated that under conditions of UVA oxidative stress, Trx prevented the UVA-induced DNA damage.
Didier
C
C
LBSO/LCR7 CEA n degrees 8--Université Joseph Fourier, Grenoble, France.
Pouget
J P
JP
Cadet
J
J
Favier
A
A
Béani
J C
JC
Richard
M J
MJ
D000080242:8-Hydroxy-2'-Deoxyguanosine; D002478:Cells, Cultured; D004249:DNA Damage; D004260:DNA Repair; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000528:radiation effects; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D005609:Free Radicals / Q000378:metabolism / Q000528:radiation effects; D015870:Gene Expression; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects / Q000528:radiation effects; D011994:Recombinant Proteins / Q000494:pharmacology; D012867:Skin / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects*; D013879:Thioredoxins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D014162:Transfection; D014466:Ultraviolet Rays / Q000009:adverse effects*
D005609:Free Radicals; D011994:Recombinant Proteins; D013879:Thioredoxins; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine