Melatonin inhibits Arg-vasopressin release via MT(2) receptor in the suprachiasmatic nucleus-slice culture of rats.
The effect of melatonin on the release of Arg-vasopressin (AVP) was analyzed in a suprachiasmatic nucleus-slice explant culture. The release of AVP into the culture medium exhibited a circadian rhythm, with higher level during the subjective day and lower level during the subjective night. Melatonin (500 nM) inhibited the release of AVP. Luzindole, a MT(2) (Mel 1b) melatonin receptor antagonist, attenuated the effect of melatonin on the AVP release. Results indicate that the inhibition of AVP release by melatonin in the suprachiasmatic nucleus-slice culture depends at least in part on the melatonin MT(2) receptor.
Isobe
Y
Y
Department of Physiology, Nagoya City University Medical School, Mizuho-ku, 467-8601, Nagoya, Japan. yisobe@med.nagoya-cu.ac.jp
Torii
T
T
Nishino
H
H
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001127:Arginine Vasopressin / Q000378:metabolism*; D002940:Circadian Rhythm / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D008550:Melatonin / Q000494:pharmacology*; D009043:Motor Activity / Q000502:physiology; D009924:Organ Culture Techniques; D017440:Photoperiod; D051381:Rats; D017208:Rats, Wistar; D011956:Receptors, Cell Surface / Q000037:antagonists & inhibitors / Q000378:metabolism*; D018160:Receptors, Cytoplasmic and Nuclear / Q000037:antagonists & inhibitors / Q000378:metabolism*; D044094:Receptors, Melatonin; D013493:Suprachiasmatic Nucleus / Q000187:drug effects / Q000378:metabolism*; D014363:Tryptamines / Q000494:pharmacology
D000975:Antioxidants; D011956:Receptors, Cell Surface; D018160:Receptors, Cytoplasmic and Nuclear; D044094:Receptors, Melatonin; D014363:Tryptamines; D001127:Arginine Vasopressin; C057154:luzindole; D008550:Melatonin