title: Values of markers of early and advanced glycation and lipoxidation in serum proteins of children with diabetes mellitus.
abstract: BACKGROUND
  Advanced glycation endproducts (AGEs) have been established as one of the major factors responsible for the multi-organ damage seen in diabetes. AGEs and lipoxidation products, as e.g. MDA, and their adducts with proteins appear to be formed together in serum and tissues. A link between AGEs formation and increased lipoxidation at tissue damage is under investigation.
  
  
  OBJECTIVE
  The aim of the present study was to determine fructosamine (FAM), glycated haemoglobin (HbA1c) AGEs-specific fluorescence and MDA-protein adducts specific fluorescence in diabetic and in healthy children, with statistical evaluation of the relationship between the parameters assessed.
  
  
  METHODS
  Values of FAM and HbA1c (spectrophotometry) and of AGEs-specific fluorescence and MDA-protein adducts specific fluorescence were investigated in serum proteins of 17 children with poorly controlled type 1 diabetes mellitus (age range 9 to 18 years). Eight healthy children (age range 7 to 17 years) served as controls.
  
  
  RESULTS
  In the diabetic group, all the parameters evaluated were significantly higher than in the control group. Furthermore, MDA-linked specific fluorescence of MDA-protein adducts (a biomarker of oxidative stress) was correlated with AGEs-specific fluorescence. In patients this correlation was extremely significant (r = 0.8176, p < 0.0001).
  
  
  CONCLUSIONS
  The increased oxidative stress in children with type 1 diabetes may not be attributed to complications, though it could contribute to the development of complications. (Tab. 2, Fig. 7, Ref. 30.)
authors.affiliation: Dpt of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Comenius University, Sasinkova 2, SK-811 08 Bratislava, Slovakia. exfakbau@nic.savba.sk
authors.forename: V
authors.initials: V
authors.lastname: Jakus
authors.forename: K
authors.initials: K
authors.lastname: Bauerova
authors.forename: D
authors.initials: D
authors.lastname: Michalkova
authors.forename: J
authors.initials: J
authors.lastname: Carsky
mesh_terms: D000293:Adolescent; D001798:Blood Proteins / Q000378:metabolism*; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000097:blood*; D019270:Fructosamine / Q000097:blood; D006442:Glycated Hemoglobin / Q000032:analysis; D017127:Glycation End Products, Advanced / Q000097:blood*; D006801:Humans; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000097:blood
chemical_list: D001798:Blood Proteins; D019270:Fructosamine; D006442:Glycated Hemoglobin; D017127:Glycation End Products, Advanced; D008315:Malondialdehyde