Antigen heterogeneity and epitope variable expression in Mycoplasma meleagridis isolates.
Immunoblotting was used to check the antigenic profiling of 27 Mycoplasma meleagridis strains isolated in different countries. Hyperimmune polyclonal rabbit antiserum as well as monoclonal antibodies (MAbs) raised against M. meleagridis (MM) showed antigen heterogeneity among strains. Five anti-MM MAbs were selected for lack of reaction against heterologous avian mycoplasma. Three of these five Mabs did not cross-react with 63 mycoplasma strains from six species affecting turkeys other than M. meleagridis. The five Mabs used to analyse the epitopes of 30 M. meleagridis strains indicated that some epitopes were not expressed in all strains. Moreover, other epitopes were located on proteins which differed according to number or molecular mass from strain to strain. The five Mabs therefore, recognised variable surface proteins, among which two were amphiphilic membrane proteins. Three of the selected Mabs recognised 29 or 30 of the 30 tested strains. The in vitro expression of surface epitopes in M. meleagridis ATCC 25284 was investigated by colony immunobinding and allowed demonstration of a variable antigenic system.
Dufour-Gesbert
F
F
Unité de mycoplasmologie bactériologie, Agence Française de Sécurité Sanitaire des Aliments, BP 53, F 22440, Ploufragan, France. f.gesbert@ploufragan.afssa.fr
Kempf
I
I
De Simone
F
F
Kobisch
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D000940:Antigenic Variation; D000942:Antigens, Bacterial / Q000096:biosynthesis* / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D018604:Epitope Mapping / Q000662:veterinary*; D007163:Immunosorbent Techniques / Q000662:veterinary; D051379:Mice; D008807:Mice, Inbred BALB C; D009174:Mycoplasma / Q000145:classification / Q000276:immunology*; D009175:Mycoplasma Infections / Q000276:immunology / Q000662:veterinary; D011817:Rabbits; D012376:Rodent Diseases / Q000175:diagnosis / Q000276:immunology
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial