title: Kynurenine aminotransferase I activity in human placenta.
abstract: The aim of the study was to detect and characterize placental kynurenine aminotransferase I (KAT I) activity in physiological pregnancy at term. Placental KAT I was inhibited by l -glutamine, l -tryptophan, and l -phenylalanine and reached optimum activity at pH 9.8. When pyruvate was used as a co-factor, the KAT I activity was significantly higher than the activity of this enzyme in the presence of 2-oxoglutarate. In the light of our findings placental KAT I seems to have biochemical characteristics of KAT I detected in human brain.
authors.affiliation: Department of Gynecology, Medical University of Lublin, Jaczewskiego 8, PL-20090 Lublin, Poland. milart@eskulap.am.lublin.pl
authors.forename: P
authors.initials: P
authors.lastname: Milart
authors.forename: E M
authors.initials: EM
authors.lastname: Urbanska
authors.forename: W A
authors.initials: WA
authors.lastname: Turski
authors.forename: T
authors.initials: T
authors.lastname: Paszkowski
authors.forename: R
authors.initials: R
authors.lastname: Sikorski
mesh_terms: D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D005973:Glutamine / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007656:Ketoglutaric Acids / Q000494:pharmacology; D007743:Labor, Obstetric / Q000378:metabolism; D008190:Lyases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010649:Phenylalanine / Q000494:pharmacology; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D019289:Pyruvic Acid / Q000494:pharmacology; D000637:Transaminases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014364:Tryptophan / Q000494:pharmacology
chemical_list: D004791:Enzyme Inhibitors; D007656:Ketoglutaric Acids; D005973:Glutamine; D010649:Phenylalanine; D019289:Pyruvic Acid; D014364:Tryptophan; D000637:Transaminases; C427605:glutamine - phenylpyruvate transaminase; C021481:kynurenine-oxoglutarate transaminase; D008190:Lyases