[Construction and expression of coexpression plasmid of carcinoembryonic antigen and Newcastle disease virus HN gene].
The eukaryotic expression plasmid (pcDNA3-CEA) containing with the gene coding for the Carcinoembryonic antigen(CEA) had been gotten with RT-PCR and gene recombination techniques. Using enzymolysis, ligation and other techniques, an eukaryotic coexpression plasmid(pcDNA3-CEA/HN) containing two expression unites of CEA and Newcastle disease virus HN gene that may have the function of immunoenhancement had been constructed. The plasmid will lay a foundation for further researching CEA nucleiotide vaccine, adjuvant and their effect of special antitumor immune.
Wei
L
L
Department of Medical Genetics, Department of Basic medicine, Second Military Medical University, Shanghai 200433.
Dai
J X
JX
Sun
S H
SH
D002272:Carcinoembryonic Antigen / Q000235:genetics*; D015707:HN Protein / Q000235:genetics*; D009522:Newcastle disease virus / Q000235:genetics*; D010957:Plasmids
D002272:Carcinoembryonic Antigen; D015707:HN Protein