Ribosome fidelity: tRNA discrimination, proofreading and induced fit.
The ribosome selects aminoacyl-tRNAs with high fidelity. Kinetic studies reveal that codon-anticodon recognition both stabilizes aminoacyl-tRNA binding on the ribosome and accelerates reactions of the productive pathway, indicating an important contribution of induced fit to substrate selection. Similar mechanisms are used by other template-programmed enzymes, such as DNA and RNA polymerases.
Rodnina
M V
MV
Institutes of Physical Biochemistry and Molecular Biology, University of Witten/Herdecke, 58448, Witten, Germany. rodnina@uni-wh.de
Wintermeyer
W
W
D003062:Codon; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D007700:Kinetics; D008954:Models, Biological; D008958:Models, Molecular; D014176:Protein Biosynthesis; D012343:RNA, Transfer / Q000378:metabolism*; D012270:Ribosomes / Q000737:chemistry* / Q000378:metabolism*
D003062:Codon; D012343:RNA, Transfer; D012321:DNA-Directed RNA Polymerases; D004259:DNA-Directed DNA Polymerase