Molecular cloning of SCDGF-B, a novel growth factor homologous to SCDGF/PDGF-C/fallotein.
Spinal cord-derived growth factor (SCDGF)/platelet-derived growth factor (PDGF)-C/fallotein has a unique two-domain structure, as it contains two regions homologousto CUB and PDGF/vascular endothelial growth factor (VEGF) domains. In this study, we isolateda novel gene homologous to SCDGF/PDGF-C/fallotein, and named SCDGF-B. The culture supernatant of CHO-K1 cells stably transfected with SCDGF-B showed mitogenic activity as SCDGF/PDGF-C/fallotein did. Although SCDGF-B and SCDGF/PDGF-C/fallotein might be the members of the PDGF/VEGF superfamily of growth factors, they were categorized into a new subfamily in addition to PDGF and VEGF subfamilies.
Hamada
T
T
Department of Pharmacology, Nippon Medical School, Bunkyo-ku, Tokyo 113-8602, Japan. thamada@nms.ac.jp
Ui-Tei
K
K
Imaki
J
J
Miyata
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D003001:Cloning, Molecular; D006224:Cricetinae; D017931:DNA Primers / Q000235:genetics; D006801:Humans; D008222:Lymphokines / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology; D008934:Mitogens / Q000494:pharmacology; D008969:Molecular Sequence Data; D010802:Phylogeny; D010982:Platelet-Derived Growth Factor / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology; D017434:Protein Structure, Tertiary; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology; D017386:Sequence Homology, Amino Acid; D014162:Transfection
D017931:DNA Primers; D008222:Lymphokines; D008934:Mitogens; D010982:Platelet-Derived Growth Factor; D011994:Recombinant Proteins; C411423:platelet-derived growth factor C