Two novel plant cDNAs homologous to animal type-2 phosphatidate phosphatase are expressed in cowpea leaves and are differently regulated by water deficits.
Two cDNAs encoding putative phosphatidate phosphatases (PAPs) designated VuPAP-alpha and VuPAP-beta were cloned in cowpea (Vigna unguiculata L.) leaves. The predicted proteins have six membrane-spanning regions in common with animal type-2 PAPs. Unlike VuPAP-beta, VuPAP-alpha has an N-terminal transit peptide and is targeted in vitro to the chloroplasts. Gene expression of VuPAP-beta is stimulated by rapid air-desiccation of leaves and VuPAP-alpha transcripts increase during rehydration of plants exposed to drought-like conditions.
Marcel
G C
GC
Laboratoire de Biochimie et Physiologie de l'Adaptation Végétale, Université Paris 7 Denis Diderot, 2 Place Jussieu, 75251 Paris Cedex 05, France.
Matos
A
A
d'arcy-Lameta
A
A
Kader
J C
JC
Zuily-Fodil
Y
Y
Pham-Thi
A
A
D000388:Air; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D002736:Chloroplasts / Q000201:enzymology; D018076:DNA, Complementary; D003890:Desiccation; D004190:Disasters; D007887:Fabaceae / Q000201:enzymology* / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D010711:Phosphatidate Phosphatase / Q000235:genetics* / Q000378:metabolism; D018515:Plant Leaves / Q000201:enzymology; D010946:Plants, Medicinal; D021381:Protein Transport; D014158:Transcription, Genetic
D018076:DNA, Complementary; D007527:Isoenzymes; D010711:Phosphatidate Phosphatase