[Schistosoma japonicum: purification and diagnostic application of recombinant 32 kD antigen].
Separation and purification of rSj32 were carried out with SDS-PAGE and electroelution technique. The purified rSj32, AWA and SEA were used to examine sera of patients with schistosomiasis, healthy individuals and patients with other parasitic diseases by ELISA. The results showed that the molecule weight of rSj32 was 37 kD. The sensitivity and specificity of rSj32 as a coating antigen in ELISA were as good as those of AWA and SEA. It is suggested that rSj32 antigen is a potential antigen for immunodiagnosis of schistosomiasis japonica.
Shu
X
X
Department of Parasitology, Hunan Medical University, Changsha 410078.
Zhou
J
J
Cai
C
C
Zeng
X
X
Zeng
Q
Q
Yi
X
X
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology / Q000302:isolation & purification*; D003429:Cross Reactions; D004011:Dicrocoeliasis / Q000175:diagnosis; D006801:Humans; D011994:Recombinant Proteins / Q000276:immunology / Q000302:isolation & purification; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000175:diagnosis
D000947:Antigens, Helminth; D011994:Recombinant Proteins