Age-related changes in cholinergic and purinergic neurotransmission in human isolated bladder smooth muscles.
We evaluated the correlation among age, cholinergic and purinergic neurotransmissions in the electrical field stimulation-induced contractions in human isolated urinary bladder smooth muscles, using the muscle bath technique. Human bladder specimens were divided into three groups (G1, under 50years; G2, 51-70years; G3, over 70years old), and each muscle strip was suspended in a thermostatically controlled organ bath filled with oxygenated Krebs-Henseleit solution, connected to an isometric force displacement transducer, and an isometric tension development was recorded. The contractile responses induced by KCl, carbachol, adenosine triphosphate (ATP) and electrical field stimulation, and the effects of atropine and alpha, beta methylene ATP on electrical field stimulation-induced contractions were observed. The contractile response to KCl and concentration-response curves for carbachol and ATP, and frequency-response curves for electrical field stimulation were not significantly different among the three groups. The atropine sensitive and resistant parts of contraction induced by electrical field stimulation were decreased and increased with age, respectively. There are significant positive and negative correlations between age and the purinergic, and age and the cholinergic neurotransmissions in human isolated bladder smooth muscles, respectively. The age-related changes in neurotransmissions may contribute to the changes in bladder function in the elderly.
Yoshida
M
M
Department of Urology, Kumamoto University School of Medicine, 1-1-1 Honjo, 860-8556, Kumamoto, Japan. masaki@kaiju.medic.kumamoto-u.ac.jp
Homma
Y
Y
Inadome
A
A
Yono
M
M
Seshita
H
H
Miyamoto
Y
Y
Murakami
S
S
Kawabe
K
K
Ueda
S
S
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000368:Aged; D000375:Aging / Q000502:physiology*; D001285:Atropine / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D002799:Cholinergic Fibers / Q000502:physiology*; D004351:Drug Resistance; D004558:Electric Stimulation; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects / Q000294:innervation*; D011189:Potassium Chloride / Q000494:pharmacology; D011687:Purines / Q000378:metabolism*; D009435:Synaptic Transmission / Q000502:physiology*; D001743:Urinary Bladder / Q000187:drug effects / Q000294:innervation*
D011687:Purines; D011189:Potassium Chloride; D001285:Atropine; D000255:Adenosine Triphosphate; D002217:Carbachol