title: Decreased susceptibility to oxidative stress-induced apoptosis of peripheral blood mononuclear cells from healthy elderly and centenarians.
abstract: The susceptibility to undergo apoptosis of fresh human peripheral blood mononuclear cells (PBMCs) from three groups of healthy donors of different ages: young people (19-40 years), old people (65-85 years) and centenarians was assessed. Apoptosis was induced by 2-deoxy-D-ribose (dRib), an agent which induces apoptosis in quiescent PBMCs by interfering with cell redox status and mitochondrial membrane potential (MMP). Our major finding is that an inverse correlation emerged between the age of the donors and the propensity of their PBMCs to undergo dRib-induced apoptosis. PBMCs from old people and centenarians also showed an increased resistance to dRib-induced glutathione depletion and a decreased tendency to lose MMP. The anti-apoptotic molecule Bcl-2 was similarly expressed in PBMCs from the three age groups. Moreover, the plasma level of the stable product of transglutaminase, epsilon(gamma-glutamyl)lysine isodipeptide, a marker of total body apoptotic rate, was decreased in centenarians compared to young and elderly people. On the whole, these findings suggest that physiological aging is characterised by a decreased tendency to undergo apoptosis, a phenomenon likely resulting from adaptation to lifelong exposure to damaging agents, such as reactive oxygen species, and may contribute to one of the major phenomena of immunosenescence, i.e. the progressive accumulation of memory/effector T cells.
authors.affiliation: Department of Experimental Pathology and Oncology, University of Florence, via Morgagni 50, 50139 Florence, Italy. daniela.monti@unifi.it
authors.forename: D
authors.initials: D
authors.lastname: Monti
authors.forename: S
authors.initials: S
authors.lastname: Salvioli
authors.forename: M
authors.initials: M
authors.lastname: Capri
authors.forename: W
authors.initials: W
authors.lastname: Malorni
authors.forename: E
authors.initials: E
authors.lastname: Straface
authors.forename: A
authors.initials: A
authors.lastname: Cossarizza
authors.forename: B
authors.initials: B
authors.lastname: Botti
authors.forename: M
authors.initials: M
authors.lastname: Piacentini
authors.forename: G
authors.initials: G
authors.lastname: Baggio
authors.forename: C
authors.initials: C
authors.lastname: Barbi
authors.forename: S
authors.initials: S
authors.lastname: Valensin
authors.forename: M
authors.initials: M
authors.lastname: Bonafè
authors.forename: C
authors.initials: C
authors.lastname: Franceschi
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000097:blood*; D017209:Apoptosis / Q000502:physiology*; D016923:Cell Death / Q000187:drug effects; D003855:Deoxyribose / Q000494:pharmacology; D004151:Dipeptides / Q000097:blood; D004351:Drug Resistance; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects; D008928:Mitochondria / Q000502:physiology; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000502:physiology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D013997:Time Factors
chemical_list: D004151:Dipeptides; D019253:Proto-Oncogene Proteins c-bcl-2; C001299:epsilon-(gamma-glutamyl)-lysine; D003855:Deoxyribose; D000111:Acetylcysteine