Purification, characterization and developmental expression of chick (Gallus domesticus) liver PSP protein.
We have isolated a perchloric acid-soluble protein designated as C-PSP from the post-mitochondria supernatant fraction of chick liver. It is soluble in 5% perchloric acid and purified by ammonium sulfate, fractionation and CM-Sephadex chromatography. The C-PSP showed approximately 70% homology with PSP isolated from rat liver (L-PSP1) with its partial amino acid sequences. The protein has a molecular mass of approximately 14 kDa which was slightly higher than that of L-PSP1. It inhibited protein synthesis in a rabbit reticulocyte lysate system. C-PSP was mainly expressed in liver and kidney and was also expressed in intestine, gizzard, glandular stomach, heart, brain and spleen though its expression was low. The expression of C-PSP in liver increased gradually from the 1st day to the 2nd week and it remained almost the same until the 13th week. C-PSP was also found in day 8 chick embryonic tissues. Interestingly, we found that C-PSP was expressed as a differentiation-dependent manner in the nervous cells of chick embryos. Thus, our findings are the first report on the presence of a PSP in avian tissues which may be involved in the regulation of cellular growth and differentiation.
Nordin
H
H
Department of Nutrition, School of Medicine, University of Tokushima, 770-8503, Tokushima, Japan.
Matsumoto
M
M
Suzuki
K
K
Kaneki
K
K
Natori
Y
Y
Kishi
K
K
Oka
T
T
D000595:Amino Acid Sequence; D000645:Ammonium Sulfate / Q000494:pharmacology; D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation; D002642:Chick Embryo; D002645:Chickens; D002845:Chromatography; D003911:Dextrans / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification*; D007150:Immunohistochemistry; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000737:chemistry* / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D010472:Perchlorates / Q000494:pharmacology; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D012260:Ribonucleases; D013997:Time Factors; D014018:Tissue Distribution
D003911:Dextrans; D006360:Heat-Shock Proteins; D010472:Perchlorates; C025614:sephadex; D012260:Ribonucleases; D000645:Ammonium Sulfate