The presence of two modes of binding to calf thymus DNA by metal-free bleomycin: a low frequency Raman study.
Double-stranded DNA is targeted by bleomycin in cancer cells and ambiguity exists as to its mode of DNA binding. A conventional Raman study was performed on drug/DNA complexes in which the low frequency spectral region (560-930 cm(-1)) was examined at two temperatures (19 and 30 degrees C). At 30 degrees C, a global Raman hypochromism was observed consistent with partial intercalation of the bithiazole moiety. At 19 degrees C, Raman hypochromism (increased base pair stacking) was detected for bands associated with GC base pairs while Raman hyperchromism (base pair destacking) was evident for bands associated with AT base pairs. These results suggest that intercalation of the bithiazole moiety occurs with greater disruption of the more efficiently stacked AT base pairs at the lower temperature. Evidence for minor groove binding was indicated by an increase in the population of bands corresponding to C3' endo sugar conformations resulting from drug induced local desolvation of the DNA polymer.
Rajani
C
C
Department of Chemistry, University of Wisconsin-Milwaukee, P. O. Box 413, Milwaukee, WI 53211, USA.
Kincaid
J R
JR
Petering
D H
DH
D000818:Animals; D001665:Binding Sites; D001761:Bleomycin / Q000737:chemistry / Q000378:metabolism*; D002417:Cattle; D004247:DNA / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D007364:Intercalating Agents / Q000737:chemistry / Q000378:metabolism; D009690:Nucleic Acid Conformation / Q000187:drug effects; D013059:Spectrum Analysis, Raman; D013696:Temperature
D007364:Intercalating Agents; D001761:Bleomycin; D004247:DNA; C427203:calf thymus DNA