Production of hydroxy fatty acids in the seeds of Arabidopsis thaliana.
Seed-specific expression in Arabidopsis thaliana of oleate hydroxylase enzymes from castor bean and Lesquerella fendleri resulted in the accumulation of hydroxy fatty acids in the seed oil. By using various Arabidopsis mutant lines it was shown that the endoplasmic reticulum (ER) n-3 desaturase (FAD3) and the FAE1 condensing enzyme are involved in the synthesis of polyunsaturated and very-long-chain hydroxy fatty acids, respectively. In Arabidopsis plants with an active ER Delta12-oleate desaturase the presence of hydroxy fatty acids corresponded to an increase in the levels of 18:1 and a decrease in 18:2 levels. Expression in yeast indicates that the castor hydroxylase also has a low level of desaturase activity.
Smith
M
M
Department of Botany, University of British Columbia, 6270 University Boulevard, Vancouver, BC, V6T 1Z4, Canada. msmith@mail.botany.ubc.ca
Moon
H
H
Kunst
L
L
D017360:Arabidopsis / Q000378:metabolism*; D002367:Ricinus communis / Q000201:enzymology / Q000235:genetics; D044943:Fatty Acid Desaturases / Q000235:genetics* / Q000378:metabolism*; D005227:Fatty Acids / Q000096:biosynthesis*; D006880:Hydroxy Acids / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000235:genetics* / Q000378:metabolism*; D010940:Plant Proteins; D030821:Plants, Genetically Modified / Q000378:metabolism*; D010947:Plants, Toxic; D012639:Seeds / Q000378:metabolism
D005227:Fatty Acids; D006880:Hydroxy Acids; D010940:Plant Proteins; D006899:Mixed Function Oxygenases; C094812:phosphatidylcholine 12-monooxygenase; D044943:Fatty Acid Desaturases; C078565:omega-3 fatty acid desaturase