Determination of glycerolipid composition of rice and maize tissues using solid-phase extraction.
Currently available techniques for the separation and characterization of different glycerolipids are complicated and/or time consuming. By modulating the stationary phase in a solid-phase extraction (SPE) manifold, efficient and rapid separation of plant membrane lipids was achieved. The glycerolipids from rice and maize tissues were separated into seven classes (monogalactosyldiacylglycerol, digalactosyldiacylglycerol, phosphatidylethanolamine, phosphatidylcholine, sulphoquinovosyldiacylglycerol, phosphatidylinositol and phosphatidylglycerol). The pigments present in the rice and maize leaves and rice stems were successfully removed from the total lipid extracts. Pigment-free plant tissue (rice roots) was also analysed. The fatty acid profile of each lipid class isolated by SPE agreed well with those obtained by other separation techniques. The recovery of glycerolipids was at least 87%.
Rizov
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I
Laboratory of Molecular Biology and Biotechnology, Mediterranean Agronomic Institute of Chania, 73100 Chania, Greece. ivzizov@hotmail.com
Doulis
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A
D005227:Fatty Acids / Q000032:analysis*; D005989:Glycerides / Q000032:analysis*; D008563:Membrane Lipids / Q000032:analysis*; D012275:Oryza / Q000737:chemistry*; D010743:Phospholipids / Q000032:analysis*; D010860:Pigments, Biological / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D012997:Solvents; D003313:Zea mays / Q000737:chemistry*
D005227:Fatty Acids; D005989:Glycerides; D008563:Membrane Lipids; D010743:Phospholipids; D010860:Pigments, Biological; D012997:Solvents