title: Interaction of pollen-specific actin-depolymerizing factor with actin.
abstract: We have examined the interaction of recombinant lily pollen ADF, LlADF1, with actin and found that whilst it bound both G- and F-actin, it had a much smaller effect on the polymerization and depolymerization rate constants than the maize vegetative ADF, ZmADF3. An antiserum specific to pollen ADF, antipADF, was raised and used to localize pollen ADF in daffodil--a plant in which massive reorganizations of the actin cytoskeleton have been seen to occur as pollen enters and exits dormancy. We show, for the first time, an ADF decorating F-actin in cells that did not result from artificial increase in ADF concentration. In dehydrated pollen this ADF : actin array is replaced by actin : ADF rodlets and aggregates of actin, which presumably act as a storage form of actin during dormancy. In germinated pollen ADF has no specific localization, except when an adhesion is made at the tip where actin and ADF now co-localize. These activities of pollen ADF are discussed with reference to the activities of ZmADF3 and other members of the ADF/cofilin group of proteins.
authors.affiliation: Department of Biological Sciences, University of Durham, South Road, Durham DH1 3LE, UK.
authors.forename: A P
authors.initials: AP
authors.lastname: Smertenko
authors.forename: E G
authors.initials: EG
authors.lastname: Allwood
authors.forename: S
authors.initials: S
authors.lastname: Khan
authors.forename: C J
authors.initials: CJ
authors.lastname: Jiang
authors.forename: S K
authors.initials: SK
authors.lastname: Maciver
authors.forename: A G
authors.initials: AG
authors.lastname: Weeds
authors.forename: P J
authors.initials: PJ
authors.lastname: Hussey
mesh_terms: D051339:Actin Depolymerizing Factors; D000199:Actins / Q000378:metabolism*; D051345:Destrin; D019684:Magnoliopsida / Q000378:metabolism; D008840:Microfilament Proteins / Q000378:metabolism*; D011058:Pollen / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D051339:Actin Depolymerizing Factors; D000199:Actins; D051345:Destrin; D008840:Microfilament Proteins; D011994:Recombinant Proteins