Species- and tissue-specific physiological regulation of vasopressin mRNA poly(A) tail length.
Transgenic experiments can be used to test the extent to which genes from different species can be swapped around, but still retain function, and be appropriately regulated. A vector has been developed that directs the expression of foreign genes to specific groups of vasopressin (VP) hypothalamic neurons in transgenic rats. Using this vector, we have expressed the bovine VP (bVP) RNA in the rat brain. In contrast to the situation in a mouse host, but like its endogenous rat counterpart, the mRNA encoded by the bVP transgene is subject to posttranscriptional physiological regulation in the hypothalamus; its poly(A) tail dramatically lengthens as a consequence of 3 days of dehydration. Transgene expression is also seen in the adrenal cortex, but here, despite a marked increase in transgene RNA levels with dehydration, there is no change in poly(A) tail length. These data suggest that the mouse hypothalamus and the rat adrenal gland do not have the transcript recognition or enzymatic machinery required for the physiologically responsive poly(A) tail length modulation seen in the rat brain.
Si-Hoe
S L
SL
Molecular Neuroendocrinology Research Group, University Research Centre for Neuroendocrinology, University of Bristol, Bristol Royal Infirmary, Bristol BS2 8HW, United Kingdom.
Carter
D
D
Murphy
D
D
D000302:Adrenal Cortex / Q000378:metabolism; D000818:Animals; D030801:Animals, Genetically Modified; D015152:Blotting, Northern; D002417:Cattle; D003681:Dehydration / Q000503:physiopathology; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D017403:In Situ Hybridization; D008297:Male; D008797:Metyrapone / Q000494:pharmacology; D051379:Mice; D011061:Poly A / Q000235:genetics*; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013045:Species Specificity; D014018:Tissue Distribution; D019076:Transgenes / Q000235:genetics; D014667:Vasopressins / Q000235:genetics*
D012333:RNA, Messenger; D014667:Vasopressins; D011061:Poly A; D008797:Metyrapone