[Experimental study on immune privilege in subretinal space].
OBJECTIVE
To evaluate the immune characteristics of subretinal space.


METHODS
Retina of DBA/2 mouse with or without Complete Freund's Adjunvate (CFA), or human peripheral blood mononuclear cell (PBMC), or syngenic retina, and DMEM as negative control were transplanted into subretinal spaces of BALB/C mouse. Delayed-type hypersensitivity (DTH) and specific killing of cytotoxic T lymphocyte (CTL) were detected two weeks later and histopathological examination was carried out as well.


RESULTS
There were no significant differences in DTH between four experimental groups and control group, but the specific killing of CTL was significantly higher than that of the control group. Retinal transplant was survival. Immune rejection occurred in human PBMC group.


CONCLUSIONS
Limited immune privilege occurs in a certain degree after subretinal space transplantation.
Gao
L
L
Department of Ophthalmology, The Second Affiliated Hospital, Hunan Medical University, Changhsa 410011.
Jiang
D
D
D000818:Animals; D005620:Freund's Adjuvant; D006084:Graft Rejection / Q000276:immunology*; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D012160:Retina / Q000637:transplantation*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
D005620:Freund's Adjuvant