Model of detergent-induced spectral changes of the B800-850 complex from Chromatium minutissimum.
The absorption and circular dichroism spectra of the B800-850 complex from Chromatium minutissimum before and after the Triton X-100 treatment were simulated by means of standard exciton theory, taking into account inhomogeneous broadening. To explain the spectral changes of the B800-850 complex treated with Triton X-100, we have assumed that all bacteriochlorophyll pigments absorbing at 850 nm exhibit the same additional rotation of approximately 20 degrees around the axis perpendicular to the membrane plane. This has been sufficient to fit the transformation in absorption and circular dichroism spectra induced by detergent treatment of the B800-850 complex.
Razjivin
A P
AP
A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russian Federation. razjivin@genebee.msu.su
Moskalenko
A A
AA
Novoderezhkin
V I
VI
D000042:Absorption / Q000187:drug effects; D002844:Chromatium / Q000737:chemistry*; D002942:Circular Dichroism; D003902:Detergents / Q000494:pharmacology*; D008954:Models, Biological; D009010:Monte Carlo Method; D017830:Octoxynol / Q000494:pharmacology*; D011487:Protein Conformation / Q000187:drug effects
D003902:Detergents; D017830:Octoxynol