title: Convenient genotyping of six myostatin mutations causing double-muscling in cattle using a multiplex oligonucleotide ligation assay.
abstract: We herein describe a procedure that allows for simultaneous genotyping of six loss-of-function mutations in the bovine myostatin gene associated with the double-muscling phenotype. The proposed method relies on a multiplex oligonucleotide ligation assay and detection of the fluorescently labelled products using automatic sequencers.
authors.affiliation: Department of Genetics, Faculty of Veterinary Medicine, University of Liège, Belgium.
authors.forename: L
authors.initials: L
authors.lastname: Karim
authors.forename: W
authors.initials: W
authors.lastname: Coppieters
authors.forename: L
authors.initials: L
authors.lastname: Grobet
authors.forename: A
authors.initials: A
authors.lastname: Valentini
authors.forename: M
authors.initials: M
authors.lastname: Georges
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D017931:DNA Primers; D005821:Genetic Techniques; D024510:Muscle Development; D009132:Muscles / Q000378:metabolism; D009154:Mutation; D055435:Myostatin; D009841:Oligonucleotides; D016212:Transforming Growth Factor beta / Q000235:genetics*
chemical_list: D017931:DNA Primers; D055435:Myostatin; D009841:Oligonucleotides; D016212:Transforming Growth Factor beta