Arenavirus nucleocapsid protein displays a transcriptional antitermination activity in vivo.
RNA polymerase pausing and transcriptional antitermination regulates gene activity in several systems. In arenavirus infected cells the switch from transcription to replication is subjected to a hairpin-dependent termination and requires protein synthesis to bypass this signal. The transcriptional antitermination control by Junín virus nucleocapsid protein N, has been demonstrated in vivo by infecting BHK-21 cells expressing this viral protein in the presence of translation inhibitors. This is the first demonstration in vivo of a transcriptional antitermination control in arenavirus-infected cells.
Tortorici
M A
MA
Instituto de Bioquímica y Biología Molecular, Depto. de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Buenos Aires, Argentina.
Albariño
C G
CG
Posik
D M
DM
Ghiringhelli
P D
PD
Lozano
M E
ME
Rivera Pomar
R
R
Romanowski
V
V
D000818:Animals; D018050:Arenavirus / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D002460:Cell Line; D006224:Cricetinae; D005057:Eukaryotic Cells / Q000821:virology*; D018051:Junin virus / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D019590:Nucleocapsid Proteins / Q000502:physiology*; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D014162:Transfection; D014779:Virus Replication / Q000235:genetics
D019590:Nucleocapsid Proteins; D012333:RNA, Messenger; D012367:RNA, Viral