Using CCM and DHPLC to detect mutations in the glucocorticoid receptor in atherosclerosis: a comparison.
Growing evidence suggests that restenosis may be caused by a failure in growth inhibitory and apoptotic systems that would normally mediate lesion regression. One such inhibitory system is the glucocorticoid receptor. This paper develops, assesses and compares chemical cleavage of mismatch (CCM) and denaturing high-performance liquid chromatography (DHPLC) for their utility in detecting mutations in this system. The results of the two methods correlated in 74% of cases in a cohort of endarterectomy patients studied by these two methods.
Cotton
R G
RG
Mutation Research Centre, St. Vincent's Hospital, 41 Victoria Parade, Victoria 3065, Fitzroy, Australia.
Bray
P J
PJ
D001161:Arteriosclerosis / Q000235:genetics* / Q000601:surgery; D020137:Base Pair Mismatch; D001483:Base Sequence; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015331:Cohort Studies; D004252:DNA Mutational Analysis / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D004691:Endarterectomy; D006801:Humans; D009691:Nucleic Acid Denaturation; D016133:Polymerase Chain Reaction; D011965:Receptors, Glucocorticoid / Q000235:genetics*; D012008:Recurrence
D017931:DNA Primers; D011965:Receptors, Glucocorticoid