Bracken (Pteridium aquilinum)-induced DNA adducts in mouse tissues are different from the adduct induced by the activated form of the Bracken carcinogen ptaquiloside.
Following treatment with bracken fern (Pteridium aquilinum) extract and bracken spores a number of DNA adducts were detected by (32)P-postlabeling. Three of these adducts have been described previously (Povey et al., Br. J. Cancer (1996) 74, 1342-1348) and in this study, using a slightly different protocol, four new adducts, with higher chromatographic mobility, were detected at levels ranging from 50 to 230% of those previously described. When DNA was treated in vitro with activated ptaquiloside (APT) and analysed by butanol extraction or nuclease P1 treatment, only one adduct was detected by (32)P-postlabeling. This adduct was not present in the DNA from mice treated with bracken fern or spores, suggesting either that bracken contains genotoxins other than ptaquiloside or that the metabolism of ptaquiloside produces genotoxins not reflected by activated ptaquiloside. However, as the ATP-derived adduct has been detected previously in ileal DNA of bracken-fed calves, species-specific differences in the metabolism of bracken genotoxins may exist, thereby leading to differences in their biological outcomes.
Freitas
R N
RN
Cancer Research Campaign Carcinogenesis Group, Paterson Institute for Cancer Research, Manchester M20 9BX, United Kingdom.
O'Connor
P J
PJ
Prakash
A S
AS
Shahin
M
M
Povey
A C
AC
D000818:Animals; D000440:Butanols; D002273:Carcinogens / Q000737:chemistry / Q000494:pharmacology*; D004247:DNA / Q000737:chemistry / Q000187:drug effects* / Q000235:genetics; D018736:DNA Adducts / Q000032:analysis* / Q000737:chemistry; D004064:Digestive System / Q000187:drug effects / Q000378:metabolism; D007189:Indans; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D012717:Sesquiterpenes; D013729:Terpenes / Q000737:chemistry / Q000494:pharmacology*
D000440:Butanols; D002273:Carcinogens; D018736:DNA Adducts; D007189:Indans; D010936:Plant Extracts; D012717:Sesquiterpenes; D013729:Terpenes; D004247:DNA; C043680:ptaquiloside