[In vitro study on arsenic trioxide-induced apoptosis of retinoic acid resistant acute promyelocytic leukemia cell line(MR-2)].
OBJECTIVE
To study the possible mechanisms of arsenic trioxide (As2O3) in the treatment of acute promyelocytic leukemia (APL).


METHODS
Retinoic acid resistant APL cell line MR-2 was used as in vitro model. The effect of As2O3 on MR-2 cell line was observed by cell viability, cell growth, cell morphology, flow cytometry assay, NBT reduction test and immunofluorescence analysis.


RESULTS
1.0 mumol/L of As2O3 could induce apoptosis of MR-2 cells and it correlated with the degradation of PML-RAR alpha fusion protein.


CONCLUSIONS
The therapeutic effect of As2O3 for APL possibly differs from that of ATRA, however, PML-RAR alpha fusion protein may be the target of both the therapy.
Cai
X
X
Shanghai Institute of Hematology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025.
Jia
P
P
Shi
X
X
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000473:pathology*; D010087:Oxides / Q000494:pharmacology*; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D001152:Arsenicals; D010087:Oxides; D014212:Tretinoin; D000077237:Arsenic Trioxide