Characterization of EDTA-soluble polysaccharides from the scape of Musa paradisiaca (banana).
The polysaccharide components present in the scape of Musa paradisiaca (banana) were fractionated into water-soluble (WSP), EDTA-soluble (EDTA-SP), alkali-soluble (ASP) and alkali-insoluble (AISP) polysaccharide fractions [Anjaneyalu, Jagadish and Raju (1997) Glycoconj. J. 14, 507-512]. The EDTA-SP was further fractionated by iso-amyl alcohol into EDTA-SP-A and EDTA-SP-B. The homogeneity of these two polysaccharides was established by repeated precipitation with iso-amyl alcohol, gel-filtration chromatography and sedimentation analysis. The polysaccharides were characterized by monosaccharide composition analysis, methylation linkage analysis, iodine affinity, ferricyanide number, blue value, hydrolysis with alpha-amylase, gold-electron microscopy and X-ray diffraction spectroscopy. Data from all of these studies suggest that EDTA-SP-A is a branched amylose-type alpha-D-glucan and that EDTA-SP-B is a highly branched amylopectin-type polymer. The nature of the branching patterns of these polysaccharides suggests that they are unique to M. paradisiaca.
Raju
T S
TS
Department of Studies in Chemistry, Manasagangotri, Mysore-570 006, India. sraju@gene.com
Jagadish
R L
RL
Anjaneyalu
Y V
YV
D002845:Chromatography / Q000379:methods; D004492:Edetic Acid; D019684:Magnoliopsida / Q000737:chemistry*; D008854:Microscopy, Electron; D011134:Polysaccharides / Q000737:chemistry* / Q000648:ultrastructure; D012995:Solubility; D013057:Spectrum Analysis
D011134:Polysaccharides; D004492:Edetic Acid