title: Isolation and identification of a diuretic hormone from Zootermopsis nevadensis.
abstract: A diuretic hormone (DH) was isolated from extracts of heads of Zootermopsis nevadensis, a dampwood termite. The peptide has 46 residues, M(r) = 5,328.2 Da, with the sequence TGAVPSLSIVNPLDVLRQRLLLEIARRRMRQSQDQIQANREMLQTI-NH(2,) showing it to be a CRF-related DH. This peptide increases cyclic AMP production in Malpighian tubules of Manduca sexta. We detected another factor in the head extracts which behaved as a more basic peptide on ion exchange chromatography. The latter factor also stimulated cyclic AMP production in the bioassay, but two large scale attempts to isolate this peptide were unsuccessful. We believe the second peptide is acid labile.
authors.affiliation: Department of Biochemistry, University of Nevada, Reno, NV 89557, USA.
authors.forename: D C
authors.initials: DC
authors.lastname: Baldwin
authors.forename: K M
authors.initials: KM
authors.lastname: Schegg
authors.forename: K
authors.initials: K
authors.lastname: Furuya
authors.forename: E
authors.initials: E
authors.lastname: Lehmberg
authors.forename: D A
authors.initials: DA
authors.lastname: Schooley
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003346:Corticotropin-Releasing Hormone / Q000032:analysis / Q000235:genetics; D007301:Insect Hormones / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification*; D020049:Isoptera; D018402:Manduca; D008969:Molecular Sequence Data; D016415:Sequence Alignment
chemical_list: D007301:Insect Hormones; D003346:Corticotropin-Releasing Hormone