[Culture of human corneal epithelial cells].
OBJECTIVE
To select culture technique of human epithelial cells for grafts.


METHODS
Epithelial cells of limbus were collected by improved enzymatic disaggregation or explant technique growth curves and to calculate doubling time. DNA synthesis of cells were measured with 3H-thymidine incorporation and liquid scintillation techniques.


RESULTS
Doubling time of cells which were collected by enzymatic disaggregation and by explant technique were 54.15 +/- 4.28 h and 67.88 +/- 1.96 h (P < 0.01). Cellular DNA synthesis of the former was more active (P < 0.01).


CONCLUSIONS
Epithelial cells which were collected by improved enzymatic disaggregation from limbus include stem cells of corneal epithelium. These cells show more active cellulde proliferation and are fitter for use as grafts.
Zhong
X
X
Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China.
Gong
X
X
Dai
Z
Z
Zheng
H
H
D018929:Cell Culture Techniques / Q000379:methods; D004247:DNA / Q000096:biosynthesis; D019573:Epithelium, Corneal / Q000166:cytology*; D006801:Humans; D016850:Limbus Corneae / Q000166:cytology
D004247:DNA