title: CLA reduces antigen-induced histamine and PGE(2) release from sensitized guinea pig tracheae.
abstract: Conjugated linoleic acid (CLA) has been shown to enhance immune reactions such as lymphocyte blastogenesis and delayed-type hypersensitivity. We investigated the role of CLA in type I (immediate) hypersensitivity, using a guinea pig tracheal superfusion model for measuring antigen-induced airway smooth muscle contraction and inflammatory mediator release. Female Hartley guinea pigs were fed a diet supplemented with 0.25 g corn oil or linoleic acid/100 g of diet (control) or 0.25 g CLA/100 g of diet for at least 1 wk before and during active sensitization to ovalbumin antigen. Tracheae from sensitized guinea pigs were suspended in air-filled water-jacketed (37 degrees C) tissue chambers in a superfusion apparatus. Tracheae were superfused with buffer containing antigen, and tissue contraction was recorded. Superfusate was collected at 90-s intervals for evaluation of histamine and PGE(2) release. CLA did not affect antigen-induced tracheal contractions when expressed as gram contraction per gram tissue. CLA significantly reduced antigen-induced histamine and PGE(2) release. CLA appears to decrease release of some inflammatory mediators during type I hypersensitivity reactions.
authors.affiliation: Department of Nutritional Sciences, Madison, Wisconsin 53706, USA.
authors.forename: L D
authors.initials: LD
authors.lastname: Whigham
authors.forename: E B
authors.initials: EB
authors.lastname: Cook
authors.forename: J L
authors.initials: JL
authors.lastname: Stahl
authors.forename: R
authors.initials: R
authors.lastname: Saban
authors.forename: D E
authors.initials: DE
authors.lastname: Bjorling
authors.forename: M W
authors.initials: MW
authors.lastname: Pariza
authors.forename: M E
authors.initials: ME
authors.lastname: Cook
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology*; D002217:Carbachol / Q000494:pharmacology; D004041:Dietary Fats / Q000008:administration & dosage; D015232:Dinoprostone / Q000378:metabolism*; D004435:Eating; D005260:Female; D006168:Guinea Pigs; D006636:Histamine Release / Q000187:drug effects*; D006969:Hypersensitivity, Immediate / Q000276:immunology* / Q000503:physiopathology; D019787:Linoleic Acid / Q000008:administration & dosage / Q000032:analysis / Q000494:pharmacology*; D009119:Muscle Contraction / Q000187:drug effects; D010047:Ovalbumin / Q000276:immunology; D014132:Trachea / Q000737:chemistry / Q000276:immunology* / Q000502:physiology*; D015430:Weight Gain
chemical_list: D000941:Antigens; D004041:Dietary Fats; D002217:Carbachol; D010047:Ovalbumin; D019787:Linoleic Acid; D015232:Dinoprostone