title: Infrequent occurrence of natural mutations in the pp65(495-503) epitope sequence presented by the HLA A*0201 allele among human cytomegalovirus isolates.
abstract: To determine if mutations of an immunodominant HLA-restricted cytomegalovirus (CMV) peptide sequence occur in nature, the sequence corresponding to the HLA A*0201-specific peptide CMVpp65(495-503) was determined in 50 human CMV isolates. Rare mutations were detected; 6 of 50 were silent mutations at the amino terminus of the peptide, while 3 of 50 were mutations of the native methionine residue to isoleucine (M499I). The observed M499I mutation in three isolates decreased cytolytic targeting.
authors.affiliation: Department of Virology, Beckman Research Institute of the City of Hope, Duarte, California 91010, USA. jzaia@coh.org
authors.forename: J A
authors.initials: JA
authors.lastname: Zaia
authors.forename: G
authors.initials: G
authors.lastname: Gallez-Hawkins
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Yao
authors.forename: N
authors.initials: N
authors.lastname: Lomeli
authors.forename: K
authors.initials: K
authors.lastname: Molinder
authors.forename: C
authors.initials: C
authors.lastname: La Rosa
authors.forename: D J
authors.initials: DJ
authors.lastname: Diamond
mesh_terms: D000483:Alleles; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology; D003587:Cytomegalovirus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000821:virology; D000939:Epitopes / Q000235:genetics*; D015234:HLA-A Antigens / Q000235:genetics* / Q000276:immunology; D006801:Humans; D009154:Mutation; D010750:Phosphoproteins / Q000235:genetics* / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014763:Viral Matrix Proteins / Q000235:genetics* / Q000276:immunology
chemical_list: D000956:Antigens, Viral; D000939:Epitopes; D015234:HLA-A Antigens; D010750:Phosphoproteins; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa