title: Focal or diffuse lesions in persistent hyperinsulinemic hypoglycemia of infancy: concerns about interpretation of intraoperative frozen sections.
abstract: Persistent hyperinsulinemic hypoglycemia of infancy (PHHI) results from defects of regulated insulin release from pancreatic beta cells and is often refractory to medical treatment. Histological changes in the pancreas associated with PHHI may be focal or diffuse, and the intraoperative confirmation and siting of focal lesions would require frozen section diagnosis. The recognition of focal involvement and its distinction from diffuse disease by frozen section depends on the identification and distribution pattern of islet cells with large hyperchromatic nuclei. This study was designed to test the feasibility of using this parameter in PHHI to delineate focal from diffuse diseases prior to the introduction of frozen sections to guide intraoperative management in our institution. A total of 66 coded and randomized paraffin sections (from 18 PHHI and 4 postmortem pancreases) were scored by three independent observers into the following categories: a focal lesion (A), no large endocrine nuclei (B), few large endocrine nuclei (C), and frequent large endocrine nuclei (D). Interobserver concordance was complete in 88%, but there were minor discrepancies in the remaining 12%. When a focal lesion was present in one section no large endocrine nuclei were seen in sections from the rest of the pancreas. In four patients with diffuse PHHI, no or only very scanty large endocrine nuclei were seen. From this finding, and the observation that in other examples of diffuse disease, large endocrine nuclei were sparse even in large paraffin sections, we have reservations about using small frozen sections for reliable diagnosis.
authors.affiliation: Department of Histopathology, Great Ormond Street Hospital for Children NHS Trust, London, UK.
authors.forename: V V
authors.initials: VV
authors.lastname: Smith
authors.forename: M
authors.initials: M
authors.lastname: Malone
authors.forename: R A
authors.initials: RA
authors.lastname: Risdon
mesh_terms: D002455:Cell Division; D002467:Cell Nucleus / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D005629:Frozen Sections / Q000379:methods*; D006801:Humans; D006946:Hyperinsulinism / Q000097:blood / Q000150:complications / Q000175:diagnosis*; D007003:Hypoglycemia / Q000097:blood / Q000150:complications / Q000175:diagnosis*; D007223:Infant; D007231:Infant, Newborn; D007432:Intraoperative Period; D007515:Islets of Langerhans / Q000378:metabolism / Q000473:pathology*; D015588:Observer Variation; D010182:Pancreatic Diseases / Q000097:blood / Q000150:complications / Q000175:diagnosis*; D011897:Random Allocation; D015203:Reproducibility of Results; D016037:Single-Blind Method