title: Immunolocalization of the Mip protein of intracellularly and extracellularly grown Legionella pneumophila.
abstract: The macrophage infectivity potentiator (Mip) protein is an important factor in the optimal intracellular survival of Legionella pneumophila in protozoa and human cell lines. In this study we have localized the Mip protein in Legionella grown on buffered charcoal yeast extract (BCYE) agar as well as in Legionella which were ingested by Acanthamoeba castellanii. Immunogold techniques have shown that Mip is exposed on the cell surface of extracellularly grown bacteria. In A. castellanii infected with Legionella the Mip protein was also detected on host membranes which exhibited a multilamellar structure. The morphology of these structures is similar to that of respirable vesicles of amoebas by which live legionellas may be transmitted to humans. It can be assumed that the accumulation of Mip protein in the multilamellar host membranes increases the infection potential.
authors.affiliation: Institut für Medizinische Mikrobiologie und Hygiene, Medizinische Fakultät, Technische Universität Dresden, Germany. Juergen.Helbig@mailbox.tu-dresden.de
authors.forename: J H
authors.initials: JH
authors.lastname: Helbig
authors.forename: P C
authors.initials: PC
authors.lastname: Lück
authors.forename: M
authors.initials: M
authors.lastname: Steinert
authors.forename: E
authors.initials: E
authors.lastname: Jacobs
authors.forename: M
authors.initials: M
authors.lastname: Witt
mesh_terms: D000048:Acanthamoeba / Q000382:microbiology* / Q000648:ultrastructure; D000818:Animals; D001426:Bacterial Proteins; D002462:Cell Membrane / Q000737:chemistry; D003470:Culture Media; D003593:Cytoplasm / Q000737:chemistry; D007150:Immunohistochemistry; D020104:Immunophilins / Q000032:analysis* / Q000276:immunology; D016952:Legionella pneumophila / Q000737:chemistry* / Q000254:growth & development* / Q000648:ultrastructure; D008565:Membrane Proteins / Q000032:analysis* / Q000276:immunology; D016253:Microscopy, Immunoelectron; D019696:Peptidylprolyl Isomerase
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D008565:Membrane Proteins; D020104:Immunophilins; C512922:Mip protein, Legionella pneumophila; D019696:Peptidylprolyl Isomerase