title: Intravital demonstration of modulation of T lymphocyte migration by CINC/gro in rat Peyer's patches.
abstract: OBJECTIVE
  Cytokine-induced neutrophil chemoattractant (CINC/gro), a member of interleukin-8 family, was found as a potent chemotactic factor for rat neutrophils. Although several chemokines have been shown to be potent regulators of T cell chemotaxis in vitro, the potential role of chemokines in T-cell migration in gut-associated lymphoid tissues has not been investigated in vivo. In the present study, the effects of CINC/gro on T-lymphocyte migration were examined in rat Peyer's patches.
  
  
  METHODS
  T lymphocytes collected from intestinal lymph of rats were fluorescence-labeled and injected into the jugular vein. Peyer's patches of the recipient rats were observed with intravital fluorescence microscopy and the effects of CINC/gro infusion was investigated. Lymphocyte flux in mesenteric collecting lymphatics was also observed.
  
  
  RESULTS
  In vivo infusion of CINC/gro significantly attenuated the initial lymphocyte interaction with postcapillary venules of Peyer's patches. However, once these lymphocytes adhered to venules, CINC/gro treatment significantly accelerated the transendothelial migration of T lymphocytes and they also significantly increased their subsequent flux in collecting lymphatics.
  
  
  CONCLUSIONS
  There is a possibility that CINC/gro could modulate the characteristics of T lymphocyte homing in the inflammatory sites of gut.
authors.affiliation: Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Fujimori
authors.forename: S
authors.initials: S
authors.lastname: Miura
authors.forename: S
authors.initials: S
authors.lastname: Koseki
authors.forename: R
authors.initials: R
authors.lastname: Hokari
authors.forename: Y
authors.initials: Y
authors.lastname: Tsuzuki
authors.forename: S
authors.initials: S
authors.lastname: Komoto
authors.forename: Y
authors.initials: Y
authors.lastname: Hara
authors.forename: H
authors.initials: H
authors.lastname: Suzuki
authors.forename: H
authors.initials: H
authors.lastname: Serizawa
authors.forename: H
authors.initials: H
authors.lastname: Ishii
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002465:Cell Movement; D054360:Chemokine CXCL1; D019743:Chemokines, CXC; D002630:Chemotactic Factors / Q000494:pharmacology*; D002633:Chemotaxis; D006131:Growth Inhibitors / Q000494:pharmacology*; D006133:Growth Substances / Q000494:pharmacology*; D007249:Inflammation; D036341:Intercellular Signaling Peptides and Proteins; D007421:Intestine, Small / Q000276:immunology* / Q000473:pathology; D008208:Lymphatic System; D008297:Male; D010581:Peyer's Patches / Q000276:immunology*; D051381:Rats; D017208:Rats, Wistar; D013601:T-Lymphocytes / Q000502:physiology*
chemical_list: D054360:Chemokine CXCL1; D019743:Chemokines, CXC; D002630:Chemotactic Factors; C513297:Cxcl1 protein, rat; D006131:Growth Inhibitors; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins