Characterization and properties of intracellular proteins after cold acclimation of the ice-nucleating bacterium Pantoea agglomerans (Erwinia herbicola) IFO12686.
The ice-nucleating bacterium Pantoea agglomerans (Erwinia herbicola) IFO12686 (INA(+)) responds to a decrease in temperature by the induction of proteins. The pattern of protein bands from strain IFO12686 following a shift in temperature from 30 to 12 degrees C could be divided into four major groups: (1) increasing protein bands, (2) decreasing protein bands, (3) increasing--decreasing protein bands, and (4) almost constant protein bands. We identified a cryoprotective function in the increasing protein band found in strain IFO12686. The increasing protein bands that followed a reduction in temperature were considered to have an important role in cold acclimation or adaptation. We showed that these proteins possessed cryoprotective activity when tested against the freeze-labile enzyme lactate dehydrogenase. The strain IFO12686 had greater cryotolerance than Pa. agglomerans IAM1595 (INA(-)), and the degree of cryotolerance was increased by cold acclimation.
Koda
N
N
Department of Biotechnology, Faculty of Engineering, Kansai University, Suita, Osaka, Japa.
Aoki
M
M
Kawahara
H
H
Yamade
K
K
Obata
H
H
D000222:Adaptation, Physiological; D001426:Bacterial Proteins / Q000737:chemistry*; D003080:Cold Temperature; D004591:Electrophoresis, Polyacrylamide Gel; D004885:Erwinia / Q000737:chemistry* / Q000201:enzymology / Q000502:physiology*; D007770:L-Lactate Dehydrogenase / Q000737:chemistry; D011489:Protein Denaturation
D001426:Bacterial Proteins; D007770:L-Lactate Dehydrogenase