Orthogonal combinatorial mutagenesis: a codon-level combinatorial mutagenesis method useful for low multiplicity and amino acid-scanning protocols.
We describe here a method to generate combinatorial libraries of oligonucleotides mutated at the codon-level, with control of the mutagenesis rate so as to create predictable binomial distributions of mutants. The method allows enrichment of the libraries with single, double or larger multiplicity of amino acid replacements by appropriate choice of the mutagenesis rate, depending on the concentration of synthetic precursors. The method makes use of two sets of deoxynucleoside-phosphoramidites bearing orthogonal protecting groups [4,4'-dimethoxytrityl (DMT) and 9-fluorenylmethoxycarbonyl (Fmoc)] in the 5' hydroxyl. These phosphoramidites are divergently combined during automated synthesis in such a way that wild-type codons are assembled with commercial DMT-deoxynucleoside-methyl-phosphoramidites while mutant codons are assembled with Fmoc-deoxynucleoside-methyl-phosphoramidites in an NNG/C fashion in a single synthesis column. This method is easily automated and suitable for low mutagenesis rates and large windows, such as those required for directed evolution and alanine scanning. Through the assembly of three oligonucleotide libraries at different mutagenesis rates, followed by cloning at the polylinker region of plasmid pUC18 and sequencing of 129 clones, we concluded that the method performs essentially as intended.
Gaytán
P
P
Unidad de Síntesis and Departamento de Reconocimiento Molecular y Bioestructura, Instituto de Biotecnología/UNAM Ap. Postal 510-3 Cuernavaca, Morelos 62250, México.
Yáñez
J
J
Sánchez
F
F
Soberón
X
X
D019943:Amino Acid Substitution / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods; D003062:Codon / Q000235:genetics*; D015723:Gene Library; D016296:Mutagenesis; D009154:Mutation; D009838:Oligodeoxyribonucleotides / Q000235:genetics
D003062:Codon; D009838:Oligodeoxyribonucleotides