[Experimental study on antitumor effect in vitro of modified DNL of oral squamous cell carcinoma with TNF-alpha gene].
The gene coding for TNF-alpha was introduced into DNL of human oral squamous carcinoma by retroviral-mediated gene transduction, and experiments proved that the gene had been introduced into the DNL and the DNL could secrete TNF-alpha of high activity with PCR analysis, bioactivity analysis of TNF-alpha, selection test in G418. Cytotoxicity of gene-transduced and nontransduced DNL to Tca8113 cell was compared with MTT method. The result showed that cytotoxicity of the former was higher than that of the latter.
Li
T
T
School of Stomatology, Shanghai Second Medical University, Shanghai 200011.
Qiu
W
W
He
R
R
D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D003602:Cytotoxicity, Immunologic; D006801:Humans; D008198:Lymph Nodes / Q000473:pathology; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology* / Q000378:metabolism; D009062:Mouth Neoplasms / Q000473:pathology*; D014062:Tongue Neoplasms / Q000473:pathology; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics*
D014409:Tumor Necrosis Factor-alpha