Phosphate-uptake systems in Yarrowia lipolytica cells grown under alkaline conditions.
In this study we used a newly isolated Yarrowia lipolytica strain with a unique capacity to grow over a wide pH range (3.5-10.5), which makes it an excellent model system for studying phosphate transport systems in cells grown under alkaline conditions. Phosphate uptake by Y. lipolytica yeast cells grown at pH 9.5-10 was shown to be mediated by several kinetically discrete Na+-dependent systems. One of these, a low-affinity transporter, operates at high Pi concentrations and is, to our knowledge, here kinetically characterized for the first time. The other two high-affinity systems are derepressible, come into play under conditions of Pi-starvation, and appear to be controlled by the availability of extracellular Pi. They represent the first examples of high-capacity, Na+-driven Pi transport systems in an organism belonging to neither the animal nor the bacterial kingdoms.
Zvyagilskaya
R
R
A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow. INBIO@online.zu
Parchomenko
O
O
Persson
B L
BL
D001692:Biological Transport; D002455:Cell Division; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010710:Phosphates / Q000493:pharmacokinetics*; D004718:Saccharomycetales / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*
D010710:Phosphates; D012964:Sodium