Molecular epidemiology of human astrovirus diarrhea among children from a periurban community of Mexico City.
Human astroviruses (HAstVs) were detected in 23 stool samples from 365 diarrhea episodes among 214 children (<18 months old) prospectively monitored for diarrhea in Mexico City. Stool samples were tested by EIA and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. EIA was less sensitive (74%) and equally specific, compared with RT-PCR analysis using type-common primers for HAstV detection. Of 31 HAstV isolates, EIA typed 18 (69%) of 26 EIA-positive samples, and RT-PCR analysis typed 26 (84%) of 31 RT-PCR-positive samples. Phylogenetic analysis of the 3' end of the capsid region (363 nucleotides) confirmed the type assignment by EIA and RT-PCR analysis and determined the type for 5 previously untyped samples. Six HAstV antigenic types cocirculated in the community: HAstV-2 (42%), HAstV-4 (23%), HAstV-3 (13%), HAstV-1 (10%), HAstV-5 (6%), and HAstV-7 (6%). RT-PCR and sequence analysis provided more detailed epidemiology of HAstV in the community than did antigenic detection methods.
Walter
J E
JE
Center for Pediatric Research, 855 West Brambleton Ave., Norfolk, VA 23510-1001, USA. walterj@chkd.com
Mitchell
D K
DK
Guerrero
M L
ML
Berke
T
T
Matson
D O
DO
Monroe
S S
SS
Pickering
L K
LK
Ruiz-Palacios
G
G
D019350:Astroviridae Infections / Q000453:epidemiology* / Q000821:virology; D001483:Base Sequence; D018938:Caco-2 Cells; D017931:DNA Primers; D003968:Diarrhea, Infantile / Q000453:epidemiology* / Q000821:virology; D004196:Disease Outbreaks; D005243:Feces / Q000821:virology; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D001257:Mamastrovirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008800:Mexico / Q000453:epidemiology; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D011446:Prospective Studies; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies; D014505:Urban Population / Q000706:statistics & numerical data
D017931:DNA Primers; D012367:RNA, Viral