title: Loss of insulator activity by paired Su(Hw) chromatin insulators.
abstract: Chromatin insulators are regulatory elements that block the action of transcriptional enhancers when interposed between enhancer and promoter. The Drosophila Suppressor of Hairy wing [Su(Hw)] protein binds the Su(Hw) insulator and prevents enhancer-promoter interaction by a mechanism that is not understood. We show that when two copies of the Su(Hw) insulator element, instead of a single one, are inserted between enhancer and promoter, insulator activity is neutralized and the enhancer-promoter interaction may instead be facilitated. This paradoxical phenomenon could be explained by interactions between protein complexes bound at the insulators.
authors.affiliation: Department of the Control of Genetic Processes, Institute of Gene Biology, Russian Academy of Sciences, Moscow 117334.
authors.forename: E
authors.initials: E
authors.lastname: Muravyova
authors.forename: A
authors.initials: A
authors.lastname: Golovnin
authors.forename: E
authors.initials: E
authors.lastname: Gracheva
authors.forename: A
authors.initials: A
authors.lastname: Parshikov
authors.forename: T
authors.initials: T
authors.lastname: Belenkaya
authors.forename: V
authors.initials: V
authors.lastname: Pirrotta
authors.forename: P
authors.initials: P
authors.lastname: Georgiev
mesh_terms: D018528:ATP-Binding Cassette Transporters; D000818:Animals; D030801:Animals, Genetically Modified; D001665:Binding Sites; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D002843:Chromatin / Q000737:chemistry / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins; D004742:Enhancer Elements, Genetic; D005127:Eye Color / Q000235:genetics; D005136:Eye Proteins / Q000235:genetics; D005786:Gene Expression Regulation; D017344:Genes, Insect; D019476:Insect Proteins / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D010858:Pigmentation; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D012097:Repressor Proteins; D018626:Retroelements
chemical_list: D018528:ATP-Binding Cassette Transporters; D002843:Chromatin; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D005136:Eye Proteins; D019476:Insect Proteins; D009687:Nuclear Proteins; D012097:Repressor Proteins; D018626:Retroelements; C083738:su(Hw) protein, Drosophila; C059254:w protein, Drosophila; C050680:y protein, Drosophila