Chemical deacylation reduces the adhesive properties of proteolipid protein and leads to decompaction of the myelin sheath.
Myelin proteolipid protein (PLP) contains thioester-bound, long-chain fatty acids which are known to influence the structure of the molecule. To gain further insights into the role of this post-translational modification, we studied the effect that chemical deacylation of PLP had on the morphology of myelin and on the protein's ability to mediate the clustering of lipid vesicles. Incubation of rat optic nerves in isoosmotic solutions containing 100 mM hydroxylamine (HA) pH 7.4 led to deacylation of PLP and decompaction of myelin lamellae at the level of the intraperiod line. Incubation of nerves with milder nucleophilic agents (Tris and methylamine) or diluted HA, conditions that do not remove protein-bound fatty acids, caused no alterations in myelin structure. Other possible effects of HA which could have affected myelin compaction indirectly were ruled out. Incubation of optic nerves with 50 mM dithioerythritol (DTE) also led to the splitting of the myelin intraperiod line and this change again coincided with the removal of fatty acids. In addition, the apparently compacted CNS myelin in the PLP-less myelin-deficient rat, like that in tissue containing deacylated PLP, was readily decompacted upon incubation in isoosmotic buffers, suggesting that the function of PLP as a stabilizer of the interlamellar attachment is, at least in part, mediated by fatty acylation. Furthermore, in contrast to the native protein, PLP deacylated with either HA or DTE failed to induce the clustering of phosphatidylcholine/cholesterol vesicles in vitro. This phenomenon is not due to side-effects of the deacylation procedure since, upon partial repalmitoylation, the protein recovered most of its original vesicle-clustering activity. Collectively, these findings suggest that palmitoylation, by influencing the adhesive properties of PLP, is important for stabilizing the multilamellar structure of myelin.
Bizzozero
O A
OA
Department of Cell Biology and Physiology, University of New Mexico-Health Sciences Center, Albuquerque, New Mexico, USA. obizzozero@salud.unm.edu
Bixler
H A
HA
Davis
J D
JD
Espinosa
A
A
Messier
A M
AM
D000215:Acylation / Q000187:drug effects; D000818:Animals; D001933:Brain Stem / Q000187:drug effects / Q000648:ultrastructure; D004226:Dithioerythritol / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D019811:Hydroxylamine / Q000494:pharmacology; D066298:In Vitro Techniques; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D008297:Male; D051379:Mice; D018991:Myelin Proteolipid Protein / Q000172:deficiency / Q000187:drug effects / Q000378:metabolism*; D009186:Myelin Sheath / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D009900:Optic Nerve / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D010169:Palmitic Acids / Q000737:chemistry; D011485:Protein Binding / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D013439:Sulfhydryl Reagents / Q000494:pharmacology; D013696:Temperature
D005227:Fatty Acids; D008081:Liposomes; D018991:Myelin Proteolipid Protein; D010169:Palmitic Acids; D013439:Sulfhydryl Reagents; D019811:Hydroxylamine; D004226:Dithioerythritol