Altered atypical coupling of gamma-aminobutyrate type A receptor agonist and convulsant binding sites in subunit-deficient mouse lines.
We searched for subunit correlations for GABA(A) receptor-associated atypically GABA-insensitive [35S]TBPS binding. The homomeric beta3 subunit receptors could be excluded, as GABA-insensitive [35S]TBPS binding was present in beta3-/- mice. Localization of GABA-insensitive [35S]TBPS binding correlated best with those of delta, alpha4 and alpha6 subunit mRNAs. The amounts of GABA-insensitive [35S]TBPS binding components were increased in delta-/- mice, but dramatically reduced in alpha6-/- mice, suggesting a role for alpha6 but excluding delta subunits.
Sinkkonen
S T
ST
Department of Pharmacology and Clinical Pharmacology, University of Turku, Kiinamyllynkatu 10, 20520, Turku, Finland.
Mihalek
R M
RM
Homanics
G E
GE
Lüddens
H
H
Korpi
E R
ER
D000818:Animals; D001665:Binding Sites; D001923:Brain Chemistry / Q000235:genetics*; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000378:metabolism / Q000494:pharmacology*; D003292:Convulsants / Q000378:metabolism / Q000494:pharmacology*; D018755:GABA Agonists / Q000378:metabolism / Q000494:pharmacology; D051379:Mice; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D011869:Radioligand Assay; D011963:Receptors, GABA-A / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013462:Sulfur Radioisotopes
D019086:Bridged Bicyclo Compounds, Heterocyclic; D003292:Convulsants; D018755:GABA Agonists; D012333:RNA, Messenger; D011963:Receptors, GABA-A; D013462:Sulfur Radioisotopes; C037476:tert-butylbicyclophosphorothionate