[The effects of transforming growth factor-beta and insulin on proliferation of human periodontal ligament cells].
OBJECTIVE
To evaluate biological effects in vitro of transforming growth factor-beta (TGF-beta) and/or insulin on proliferation of the human periodontal ligament (PDL) cells.


METHODS
Cell cultures and MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] colorimetric assay.


RESULTS
TGF-beta at concentrations of 0.1 to 10 micrograms/L and insulin at 10 to 1,000 U/L had significant proliferative effects on human PDL cells (P < 0.01). From the 3rd day, the significant proliferation of PDL cells by TGF-beta or insulin were observed (P < 0.01) and lasted to the 5th day. The effect of combination of TGF-beta with insulin was the most significant one (P < 0.01).


CONCLUSIONS
TGF-beta and/or insulin may be valuable in promoting the regeneration of periodontal connective tissue.
Wu
Z
Z
School of Stomatology, Fourth Military Medical University, Xi'an 710032.
Dong
G
G
Wan
L
L
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004357:Drug Synergism; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D010513:Periodontal Ligament / Q000166:cytology* / Q000187:drug effects; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D007328:Insulin; D016212:Transforming Growth Factor beta