[Microbial test combination with Vibrio fischeri as a screening toxicity test]
Two different microbial toxicity tests were investigated concerning the possibility to reduce animal experiments in product testing. In the acute bioluminescence test the inhibition of the bacterial luminescence was measured. In the chronic DMS test the inhibition of the microbial metabolism of DMSO to DMS was recorded. The combination of both tests with the same organism Vibrio fischeri enhances the power as a toxicological indicator. We used well known alcohols and tensides. For 15 substances, the correlation between the experimental results and the Draize eye irritation test (rs = 0,943) and the intravenous toxicity (rs = 0,958) was statistically significant. If both tests gave EC50-values >10000mg/L, the substance could be categorised as non or mild toxic. If the EC50-values were <5mg/L, an extremely toxic substance could be expected. Neither false positive nor false negative results have been found, therefore the application of the method as a screening test or as a part in a test battery is conceivable.
Schicktanz
Silke
S
Eberhard-Karls-Universität D-Tübingen, Lehrstuhl für Ethik in den Biowissenschaften.
Frahne
Dietrich
D
Honnen
Wolfgang
W
Müller
Ewald
E