title: Flow cytometric testing of susceptibilities of Mycobacterium avium to amikacin, ciprofloxacin, clarithromycin and rifabutin in 24 hours.
abstract: OBJECTIVE
  To develop a biologically safe flow cytometric susceptibility test that depends on detection and enumeration of actively growing Mycobacterium avium organisms in drug-free and antimycobacterial agent-containing medium.
  
  
  METHODS
  Prior to analysis by flow cytometry, all M. avium susceptibility test samples were inactivated by exposure to paraformaldehyde. The susceptibilities of 20 clinical isolates of M. avium to amikacin, ciprofloxacin, clarithromycin, and rifabutin were tested by the flow cytometric and BACTEC methods.
  
  
  RESULTS
  Agreement was 97% between the results of the two methods. The results of flow cytometric susceptibility tests were available 24 h after inoculation of drug-containing medium, while the BACTEC method required 4-8 days to complete.
  
  
  CONCLUSIONS
  The flow cytometric assay is safe, simple and reproducible.
authors.affiliation: Wisconsin State Laboratory of Hygiene, Madison, Wisconsin, USA.
authors.forename: R M
authors.initials: RM
authors.lastname: Vena
authors.forename: E L
authors.initials: EL
authors.lastname: Munson
authors.forename: D J
authors.initials: DJ
authors.lastname: DeCoster
authors.forename: C L
authors.initials: CL
authors.lastname: Croke
authors.forename: D B
authors.initials: DB
authors.lastname: Fett
authors.forename: S M
authors.initials: SM
authors.lastname: Callister
authors.forename: R F
authors.initials: RF
authors.lastname: Schell
mesh_terms: D004352:Drug Resistance, Microbial; D005434:Flow Cytometry; D008826:Microbial Sensitivity Tests / Q000379:methods*; D009162:Mycobacterium avium / Q000187:drug effects*; D015203:Reproducibility of Results