title: Protective efficacy of vaccination by recombinant vaccinia virus against Neospora caninum infection.
abstract: The recombinant vaccinia viruses expressing the surface protein of Neospora caninum tachyzoite, NcSAG1 or NcSRS2, were constructed. The vaccination with these recombinant viruses could protect effectively the parasite invasion in a mouse model system. The vaccine efficacy of NcSRS2 was higher than that of NcSAG1. The present study indicated that a high level of IgG1 Ab production to parasite is important for clearance of parasite at the early stage of infection and that T cell response has a crucial role for protection against the intracellular infection at the late stage of infection. The recombinant vaccinia viruses might be applicable as vaccine against N. caninum infection in a natural host.
authors.affiliation: The National Research Center for Protozoan Diseases, Obihiro University, Nishi 2-11, Inada-cho, Obihiro, 080-8555, Hokkaido, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Nishikawa
authors.forename: N
authors.initials: N
authors.lastname: Inoue
authors.forename: X
authors.initials: X
authors.lastname: Xuan
authors.forename: H
authors.initials: H
authors.lastname: Nagasawa
authors.forename: I
authors.initials: I
authors.lastname: Igarashi
authors.forename: K
authors.initials: K
authors.lastname: Fujisaki
authors.forename: H
authors.initials: H
authors.lastname: Otsuka
authors.forename: T
authors.initials: T
authors.lastname: Mikami
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000913:Antibodies, Protozoan / Q000096:biosynthesis / Q000494:pharmacology; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D002460:Cell Line; D003048:Coccidiosis / Q000235:genetics / Q000276:immunology* / Q000517:prevention & control*; D016207:Cytokines / Q000096:biosynthesis; D016054:DNA, Protozoan / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006801:Humans; D007053:Ice; D007371:Interferon-gamma / Q000096:biosynthesis; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D018688:Neospora; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology; D016052:Protozoan Vaccines / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology*; D013154:Spleen / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology / Q000494:pharmacology; D014616:Vaccinia virus / Q000235:genetics* / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D016207:Cytokines; D016054:DNA, Protozoan; D007053:Ice; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; C115389:SRS2 protein, Neospora caninum; D014614:Vaccines, Synthetic; D007371:Interferon-gamma