In vitro exposure to highly cytopathic HIV-1 X4 strains increases expression of mucosa-associated integrins on CD4(+) T cells.
To determine whether infection with HIV-1 strains of different tropisms would influence expression of the mucosa-associated integrins alpha 4 beta 7 and alpha E beta 7 or the lymph node homing receptor L-selectin on peripheral T lymphocytes, cells were infected with the CXCR4-tropic (X4)/syncytium-inducing (SI) HIV-1(IIIB) strain or with X4/SI or CCR5-tropic (R5)/non-SI (NSI) primary human isolates. Flow cytometric analyses of CD4(+) T cells from cultures infected with HIV-1(IIIB) and one X4/SI primary HIV-1 isolate revealed a significant increase in surface expression of alpha 4 beta 7 and alpha E beta 7 12 days after infection. L-selectin expression was not significantly affected on CD4(+) T cells. However, infection with another X4/SI and two R5/NSI primary HIV-1 isolates did not significantly alter homing receptor expression on CD4(+) T cells. Since a higher degree of CD4 cytopathicity occurred in those cultures having increased integrin expression, these data suggest that significantly altered mucosal homing receptor expression on CD4(+) T cells may result as a "bystander" effect after infection with some cytopathic isolates of HIV-1.
Cummins
J E
JE
Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA. jcummins0@cdc.gov
Bunn
W J
WJ
Hall
S D
SD
Donze
H H
HH
Mestecky
J
J
Jackson
S
S
D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism* / Q000821:virology; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D015658:HIV Infections / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology* / Q000472:pathogenicity / Q000502:physiology; D006801:Humans; D016023:Integrins / Q000096:biosynthesis*; D019041:L-Selectin / Q000096:biosynthesis*; D009092:Mucous Membrane / Q000378:metabolism
D016023:Integrins; C071777:integrin alpha4beta7; C070709:integrin alphaEbeta7; D019041:L-Selectin