title: Effect of collagenase inhibitors on corneal haze after PRK.
abstract: The aim of the study was to determine whether collagenase inhibitors reduce corneal haze after photorefractive keratectomy (PRK). Inhibition of the initial removal phase of healing may limit the subsequent repair and replacement phases responsible for haze and regression. Thirty rabbits received -6.00D 5 mm right PRK. They were randomized to five treatment groups: G. cysteine, G. ethylene diamine tetra-acetic acid (EDTA), G. ascorbate, Oc. tetracycline or no drops. Dichlorotriazinyl aminofluorescein (DTAF) was applied to the wound immediately after surgery in two rabbits of each group, to delineate newly-synthesized from original tissue. Corneal haze was assessed by a video-linked frame grabber with computerized grey scale analysis. Corneas were taken for histology at 1 or 3 months post-operatively. Corneal haze was not significantly different between the treatment groups and controls. The severity of the histological changes varied between individuals. Within the ablation zone the epithelium was on average 10% thicker (3--4 micro m) than outside, and in some rabbits there were irregularities of the epithelial--stromal junction. The new subepithelial tissue had a mean depth of 7.8 micro m, and the superficial stroma was disorganized to a mean depth of 49 micro m. No particular treatment demonstrated significant benefits over controls; but of the treatments used, cysteine tended to produce the best results. Eyes treated with EDTA fared worst in most respects. The collagenase inhibitors used did not improve the outcome of PRK in rabbits. It remains to be determined whether firstly, the new more potent agents would have an effect, and secondly, whether collagenase inhibitors are of benefit in humans.
authors.affiliation: Department of Ophthalmology, St. Thomas' Hospital, London, UK.
authors.forename: M C
authors.initials: MC
authors.lastname: Corbett
authors.forename: D P
authors.initials: DP
authors.lastname: O'Brart
authors.forename: A L
authors.initials: AL
authors.lastname: Patmore
authors.forename: J
authors.initials: J
authors.lastname: Marshall
mesh_terms: D000287:Administration, Topical; D000818:Animals; D001205:Ascorbic Acid / Q000627:therapeutic use; D003315:Cornea / Q000187:drug effects* / Q000473:pathology; D003545:Cysteine / Q000627:therapeutic use; D004492:Edetic Acid / Q000627:therapeutic use; D004791:Enzyme Inhibitors / Q000627:therapeutic use*; D019573:Epithelium, Corneal / Q000503:physiopathology; D054018:Lasers, Excimer; D061965:Matrix Metalloproteinase Inhibitors; D018903:Photorefractive Keratectomy; D011817:Rabbits; D013752:Tetracycline / Q000627:therapeutic use; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects
chemical_list: D004791:Enzyme Inhibitors; D061965:Matrix Metalloproteinase Inhibitors; D004492:Edetic Acid; D013752:Tetracycline; D003545:Cysteine; D001205:Ascorbic Acid