title: Caseins and casein hydrolysates. 1. Lipoxygenase inhibitory properties.
abstract: Whole casein from bovine origin, the different casein subtypes alpha, beta, and kappa, and the related dephosphorylated proteins were assayed as modulators of soybean lipoxygenase 1 activity and were found to inhibit it. To define the lipoxygenase inhibitory domain, whole casein and beta-casein were digested by proteases (trypsin, clostripain, and subtilisin). The beta-casein tryptic digest and the tryptic and subtilisin digests of whole casein retained their inhibitory properties. The tryptic beta-casein digest was the most potent inhibitor of lipoxygenase activity and was further fractionated by FPLC or HPLC. The collected peptides inhibited the lipoxygenase-catalyzed reaction to different extents. The active fractions were analyzed by ESI-MS, and the sequences of several lipoxygenase inhibitory peptides, corresponding mainly to the C-terminal moiety of beta-casein, were identified.
authors.affiliation: Agrotechnological Research Institute (ATO), P.O. Box 17, 6700 AA Wageningen, The Netherlands.
authors.forename: S G
authors.initials: SG
authors.lastname: Rival
authors.forename: S
authors.initials: S
authors.lastname: Fornaroli
authors.forename: C G
authors.initials: CG
authors.lastname: Boeriu
authors.forename: H J
authors.initials: HJ
authors.lastname: Wichers
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002364:Caseins / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003546:Cysteine Endopeptidases / Q000378:metabolism; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology*; D010446:Peptide Fragments / Q000737:chemistry / Q000494:pharmacology; D020539:Sequence Analysis, Protein; D013025:Glycine max / Q000201:enzymology; D021241:Spectrometry, Mass, Electrospray Ionization; D020860:Subtilisin / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism
chemical_list: D002364:Caseins; D016859:Lipoxygenase Inhibitors; D010446:Peptide Fragments; D014357:Trypsin; D020860:Subtilisin; D003546:Cysteine Endopeptidases; C019913:clostripain