[The study of radioresistance of BHRF1 gene].
In order to learn relationship between the expression of BHRF1 gene and radiative sensitivity of cells of nasopharyngeal carcinoma. High BHRF1 expressive vector was set up and transfected in the cell line of nasopharyngeal carcinoma, CNE2. Then the cells were radiated to test its ability of clone formation in nude mice. The results showed that the tumors formed by BHRF1-CNE2 cells were lighter before radiation and weighter after radiation (P < 0.01), and apoptotic index of BHRF1-CHE2 was less than control groups (P < 0.01). So, it suggests that the BHRF1 expression could prevent apoptosis by enhancing the resistibility of the tumor cells to radiation.
Huang
H
H
Changhai Hospital, Second Military Medical University, Shanghai 200433.
Pan
X
X
Kong
X
X
D000818:Animals; D017209:Apoptosis; D003037:Cobalt Radioisotopes; D004249:DNA Damage / Q000528:radiation effects; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000473:pathology*; D009368:Neoplasm Transplantation; D011836:Radiation Tolerance; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000528:radiation effects; D014764:Viral Proteins / Q000235:genetics*
C083278:BHRF1 protein, Human herpesvirus 4; D003037:Cobalt Radioisotopes; D014764:Viral Proteins