Prevention of vertical transmission of Neospora caninum in BALB/c mice by recombinant vaccinia virus carrying NcSRS2 gene.
Neospora caninum infection is the major cause of bovine abortion. To develop a vaccine against N. caninum infection, recombinant vaccinia viruses carrying NcSRS2 and NcSAG1 genes (vv/Nc-p43 and vv/Nc-p36, respectively) were constructed and were tested in a mouse model. Vaccination of dams with vv/Nc-p43 appeared to confer effective protection against vertical transmission to offspring, though that with vv/Nc-p36 only provided partial protection. Moreover, the vv/Nc-p43 vaccination provoked cellular immune responses and antibody production against N. caninum. In conclusion, it is expected that vv/Nc-p43 can be used as an effective live vaccine to prevent vertical transmission of N. caninum in natural hosts.
Nishikawa
Y
Y
The National Research Center for Protozoan Diseases, Obihiro University, Nishi 2-11, Inadacho, Obihiro, 080-8555, Hokkaido, Japan.
Xuan
X
X
Nagasawa
H
H
Igarashi
I
I
Fujisaki
K
K
Otsuka
H
H
Mikami
T
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000913:Antibodies, Protozoan / Q000276:immunology; D000918:Antibody Specificity; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology; D003048:Coccidiosis / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control* / Q000635:transmission; D003208:Concanavalin A / Q000494:pharmacology; D016054:DNA, Protozoan / Q000032:analysis; D005260:Female; D017125:Genes, Protozoan / Q000235:genetics*; D018445:Infectious Disease Transmission, Vertical; D008098:Litter Size; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018688:Neospora / Q000235:genetics / Q000276:immunology*; D011247:Pregnancy; D016052:Protozoan Vaccines / Q000235:genetics / Q000276:immunology*; D013154:Spleen / Q000187:drug effects / Q000276:immunology; D015996:Survival Rate; D013601:T-Lymphocytes / Q000276:immunology; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology*; D014616:Vaccinia virus / Q000235:genetics*
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D016052:Protozoan Vaccines; D014614:Vaccines, Synthetic; D003208:Concanavalin A