Characterization of hepatitis B virus genotypes among Yucpa Indians in Venezuela.
The complete genome sequences of hepatitis B virus (HBV) from 12 HBV-infected Yucpa Indians of Venezuela, a group with highly endemic HBV, were amplified and sequenced. The 12 isolates were closely related to each other, with 98.6-100% nucleotide identity. A phylogenetic tree based on the complete genome indicated clearly that they were genotype F. Three individuals had evidence of infection with two different HBV deletion mutants. In two individuals, a three amino acid deletion was identified just prior to the 'a' determinant loop of the S region. A third individual was infected with virus that contained a complete core reading frame and a population that contained a deletion in the middle of the core region. These results indicate that genotype F HBV is present in the Venezuelan Yucpa Amerindians and the complete genome sequence allowed the identification of two unique deletion mutants in a limited set of samples.
Nakano
Tatsunori
T
Second Department of Medicine, Nagoya City University Medical School, Nagoya 467-8601, Japan2.|Hepatitis Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA1.
Lu
Ling
L
Hepatitis Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA1.
Hu
Xiaolei
X
Hepatitis Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA1.
Mizokami
Masashi
M
Second Department of Medicine, Nagoya City University Medical School, Nagoya 467-8601, Japan2.
Orito
Etsuro
E
Second Department of Medicine, Nagoya City University Medical School, Nagoya 467-8601, Japan2.
Shapiro
Craig N
CN
Hepatitis Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA1.
Hadler
Stephan C
SC
Hepatitis Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA1.
Robertson
Betty H
BH
Hepatitis Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA1.
D000595:Amino Acid Sequence; D016384:Consensus Sequence; D016679:Genome, Viral; D005838:Genotype; D006509:Hepatitis B / Q000453:epidemiology / Q000821:virology*; D006514:Hepatitis B Surface Antigens / Q000737:chemistry / Q000235:genetics; D006515:Hepatitis B virus / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D006801:Humans; D007199:Indians, South American; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D017384:Sequence Deletion / Q000235:genetics; D014687:Venezuela / Q000453:epidemiology; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
D006514:Hepatitis B Surface Antigens; D014764:Viral Proteins