Application of a new method for the sensitive detection and quantification of acyl-CoA esters in Arabidopsis thaliana seedlings and mature leaves.
We report a novel, highly sensitive and selective method for the detection and quantification of acyl-CoA esters from Arabidopsis thaliana. Extracted acyl-CoA esters were derived to their fluorescent acyl-etheno-CoA esters, separated by ion-paired reversed-phase HPLC, and detected fluorometrically. We report the composition and concentration of the acyl-CoA pool in mature A. thaliana leaves, and during storage-lipid breakdown in 2-day-old seedlings. The concentrations measured were in the 1-4 microM range for both tissue types. To our knowledge, this is the first time that reliable estimates of acyl-CoA ester concentrations have been made for plants.
Larson
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Centre for Novel Agricultural Products, Central Science Laboratory, Sand Hutton, York YO41 1LZ, U.K.
Graham
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D000214:Acyl Coenzyme A / Q000032:analysis*; D017360:Arabidopsis / Q000737:chemistry* / Q000254:growth & development; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005456:Fluorescent Dyes; D018515:Plant Leaves / Q000737:chemistry; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
D000214:Acyl Coenzyme A; D005456:Fluorescent Dyes