title: Adr1p-dependent regulation of the oleic acid-inducible yeast gene SPS19 encoding the peroxisomal beta-oxidation auxiliary enzyme 2,4-dienoyl-CoA reductase.
abstract: The role of Saccharomyces cerevisiae Adr1p was examined with respect to the transcriptional regulation of the SPS19 gene encoding the peroxisomal beta-oxidation auxiliary enzyme 2,4-dienoyl-CoA reductase. The SPS19 promoter contains both an oleate response element that binds the Pip2p-Oaf1p transcription factor as well as a canonical Adr1p-binding element, termed UAS1(SPS19). Northern analysis demonstrated that transcriptional up-regulation of SPS19 was abolished in cells devoid of Adr1p. Expression of an SPS19-lacZ reporter gene was shown to be quiescent in the adr1Delta mutant and abnormally elevated in cells containing multiple ADR1 copies. UAS1(SPS19) was able to compete for formation of a specific complex between recombinant Adr1p-LacZ and UAS1(CTA1) representing the corresponding Adr1p-binding element in the promoter of the catalase A gene, and to interact directly with this fusion protein. We conclude that in the presence of fatty acids in the medium transcription of SPS19 is directly regulated by both Pip2p-Oaf1p and Adr1p.
authors.affiliation: Institut für Biochemie und Molekulare Zellbiologie, Universität Wien and Ludwig Boltzmann-Forschungsstelle für Biochemie, Vienna Biocenter, Dr Bohrgasse 9, A-1030 Vienna, Austria. AG@abc.univie.ac.at
authors.forename: A
authors.initials: A
authors.lastname: Gurvitz
authors.forename: L
authors.initials: L
authors.lastname: Wabnegger
authors.forename: H
authors.initials: H
authors.lastname: Rottensteiner
authors.forename: I W
authors.initials: IW
authors.lastname: Dawes
authors.forename: A
authors.initials: A
authors.lastname: Hartig
authors.forename: H
authors.initials: H
authors.lastname: Ruis
authors.forename: B
authors.initials: B
authors.lastname: Hamilton
mesh_terms: D001665:Binding Sites; D015152:Blotting, Northern; D017931:DNA Primers / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000494:pharmacology*; D004587:Electrophoresis, Agar Gel; D044943:Fatty Acid Desaturases / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D005822:Genetic Vectors; D007763:Lac Operon / Q000502:physiology; D019301:Oleic Acid / Q000378:metabolism*; D044925:Oxidoreductases Acting on CH-CH Group Donors; D020675:Peroxisomes / Q000201:enzymology* / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins; D013172:Spores, Fungal; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology*; D015533:Transcriptional Activation; D015854:Up-Regulation; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: C074682:ADR1 protein, S cerevisiae; D017931:DNA Primers; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D019301:Oleic Acid; D044943:Fatty Acid Desaturases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C100153:2,4-dienoyl-CoA reductase; D001616:beta-Galactosidase