title: Glycosylphosphatidylinositol-anchored proteins found in the moss Marchantia polymorpha.
abstract: A suspension culture of the moss Marchantia polymorpha was incubated with either [3H]ethanolamine or [3H]myristic acid, precursors of a glycosylphosphatidylinositol (GPI) anchor. By both of these a secretory protein of 47 kDa was consistently labelled. Amino acid composition analysis of the chromatographically purified 47-kDa protein exhibited the occurrence of ethanolamine and glucosamine at approximate molar ratios to protein of 1 and 4, respectively. Thus the secretory 47-kDa protein of M. polymorpha was concluded to be a GPI-anchored protein.
authors.affiliation: Graduate School Environmental Earth Science, Hokkaido University, Sapporo 060-0810, Japan. hoku@ees.hokudai.ac.jp
authors.forename: H
authors.initials: H
authors.lastname: Okuyama
authors.forename: S
authors.initials: S
authors.lastname: Hagiwara
authors.forename: T
authors.initials: T
authors.lastname: Okamoto
authors.forename: K
authors.initials: K
authors.lastname: Kato
mesh_terms: D019068:Bryopsida / Q000737:chemistry* / Q000378:metabolism*; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D019856:Ethanolamine / Q000378:metabolism; D017261:Glycosylphosphatidylinositols / Q000032:analysis* / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008970:Molecular Weight; D019814:Myristic Acid / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D014316:Tritium
chemical_list: D017261:Glycosylphosphatidylinositols; D008565:Membrane Proteins; D010940:Plant Proteins; D019814:Myristic Acid; D014316:Tritium; D019856:Ethanolamine