Tissue factor is the only activator of coagulation in cultured human lung cancer cells.
It is a long-known principle that tumour cells tend to exploit the host's physiologic systems in order to get support in terms of, for example, nutrition, growth or metastasis. One of these physiologic systems is the blood coagulation cascade, which has been found activated in many tumour patients. The mechanisms of the activation of coagulation have been assessed in numerous animal and in vitro experiments, and the results appeared to point to several distinct activators. The present study used a large panel of different cultivated human lung cancer cell lines and experimental systems involving normal plasma, plasmas deficient of factors V, VII or X, purified coagulation factors II and X, recombinant tissue factor (TF), and specific inhibitory antibodies against factor VII and TF. The results provide strong evidence that there is no activator of coagulation besides TF in the wide array of lung cancer cells examined. However, this work reveals a striking variability of TF content among the cell lines. This might explain ambiguous results of clinical trials of anticoagulation as an adjunct to antineoplastic therapy in lung cancer. By sensitive diagnostic tools like the plasma thrombin-antithrombin complex levels it might be possible to select patients with activated coagulation, who might benefit from anticoagulation.
Keller
T
T
Department of Haematology and Transfusion Medicine, Paul-Ehrlich-Institut, Paul-Ehrlich-Strasse 51-59, D-63225 Langen, Germany.
Salge
U
U
König
H
H
Dodt
J
J
Heiden
M
M
Seitz
R
R
D000906:Antibodies / Q000276:immunology; D001777:Blood Coagulation / Q000502:physiology*; D005165:Factor V / Q000494:pharmacology; D005167:Factor VII / Q000494:pharmacology; D005170:Factor X / Q000494:pharmacology; D006801:Humans; D008175:Lung Neoplasms / Q000276:immunology / Q000503:physiopathology*; D013917:Thrombin / Q000032:analysis; D013925:Thromboplastin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000906:Antibodies; D005165:Factor V; D005167:Factor VII; D005170:Factor X; D013925:Thromboplastin; D013917:Thrombin