title: Characterization and properties of intracellular proteins after cold acclimation of the ice-nucleating bacterium Pantoea agglomerans (Erwinia herbicola) IFO12686.
abstract: The ice-nucleating bacterium Pantoea agglomerans (Erwinia herbicola) IFO12686 (INA(+)) responds to a decrease in temperature by the induction of proteins. The pattern of protein bands from strain IFO12686 following a shift in temperature from 30 to 12 degrees C could be divided into four major groups: (1) increasing protein bands, (2) decreasing protein bands, (3) increasing--decreasing protein bands, and (4) almost constant protein bands. We identified a cryoprotective function in the increasing protein band found in strain IFO12686. The increasing protein bands that followed a reduction in temperature were considered to have an important role in cold acclimation or adaptation. We showed that these proteins possessed cryoprotective activity when tested against the freeze-labile enzyme lactate dehydrogenase. The strain IFO12686 had greater cryotolerance than Pa. agglomerans IAM1595 (INA(-)), and the degree of cryotolerance was increased by cold acclimation.
authors.affiliation: Department of Biotechnology, Faculty of Engineering, Kansai University, Suita, Osaka, Japa.
authors.forename: N
authors.initials: N
authors.lastname: Koda
authors.forename: M
authors.initials: M
authors.lastname: Aoki
authors.forename: H
authors.initials: H
authors.lastname: Kawahara
authors.forename: K
authors.initials: K
authors.lastname: Yamade
authors.forename: H
authors.initials: H
authors.lastname: Obata
mesh_terms: D000222:Adaptation, Physiological; D001426:Bacterial Proteins / Q000737:chemistry*; D003080:Cold Temperature; D004591:Electrophoresis, Polyacrylamide Gel; D004885:Erwinia / Q000737:chemistry* / Q000201:enzymology / Q000502:physiology*; D007770:L-Lactate Dehydrogenase / Q000737:chemistry; D011489:Protein Denaturation
chemical_list: D001426:Bacterial Proteins; D007770:L-Lactate Dehydrogenase