[Polymerase chain reaction applied for detection of Toxoplasma gondii in lymph nodes].
In order to investigate the morbidity of toxoplasmic lymphadenitis, polymerase chain reaction (PCR) was used to detect DNA of Toxoplasma gondii within lymph nodes in 3 groups of 120 patients with different diseases. After extracting the DNA of each sample, PCR was employed to amplify toxoplasma DNA. The results showed that the amplification product of 210 bp was confirmed in 7 patients: 3 cases of Hodgkin's disease (HD), 2 cases of non-Hodgkin's lymphoma (NHL) and 2 cases of chronic lymphadenitis (CL). Each PCR product was then subjected to Southern blot hybridization. Besides the 7 cases proved by PCR, 1 case of CL was found positive. The positive percentages of HD, NHL and CL were 9.38% (3/32), 4.88% (2/41), and 6.38% (3/47), respectively. The total positive rate was 6.67% (8/120).
Liu
C
C
Department of Infectious Diseases, Xiangya Hospital, Hunan Medical University, Changsha 410008.
Ouyang
K
K
Tan
D
D
D000818:Animals; D002908:Chronic Disease; D016054:DNA, Protozoan / Q000302:isolation & purification*; D005260:Female; D006689:Hodgkin Disease / Q000469:parasitology; D006801:Humans; D008198:Lymph Nodes / Q000469:parasitology*; D008199:Lymphadenitis / Q000469:parasitology*; D008228:Lymphoma, Non-Hodgkin / Q000469:parasitology; D008297:Male; D016133:Polymerase Chain Reaction; D014122:Toxoplasma / Q000302:isolation & purification*; D014123:Toxoplasmosis / Q000469:parasitology
D016054:DNA, Protozoan