Purification of a novel myofibril-bound serine proteinase inhibitor (MBSPI) from the skeletal muscle of lizard fish.
A novel myofibril-bound serine proteinase inhibitor (MBSPI) was purified to homogeneity from the skeletal muscle of lizard fish (Saurida wanieso). Purification was carried out by ammonium sulfate fractionation, followed by column chromatographies on DEAE-Sephacel, SP-Sepharose and Sephadex G-150. MBSPI was purified 7.7-fold starting from the DEAE-Sephacel fraction, with a yield of 0.2%. It is a monomeric protein with the molecular mass of 50 kDa as estimated by SDS-PAGE and gel filtration. MBSPI reveals high inhibition specificity toward a myofibril-bound serine proteinase (MBSP) purified from lizard fish muscle. No inhibition is detected toward bovine trypsin, bovine chymotrypsin, two trypsins from carp hepatopancreas and a serine proteinase isolated from the sarcoplasmic fraction of white croaker muscle. It does not exert any inhibitory activity toward a myofibril-bound serine proteinase from carp muscle.
Cao
M J
MJ
Graduate School of Marine Science and Engineering, Nagasaki University, Bunkyo, 852-8521, Nagasaki, Japan.
Osatomi
K
K
Hara
K
K
Ishihara
T
T
D000645:Ammonium Sulfate / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D002847:Chromatography, Agarose; D002850:Chromatography, Gel; D002918:Chymotrypsin / Q000378:metabolism; D003911:Dextrans / Q000494:pharmacology; D004064:Digestive System / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004983:Ethanolamines / Q000494:pharmacology; D005399:Fishes; D008970:Molecular Weight; D018482:Muscle, Skeletal / Q000737:chemistry*; D009210:Myofibrils / Q000737:chemistry*; D012519:Sarcoplasmic Reticulum / Q000378:metabolism; D012697:Serine Endopeptidases / Q000378:metabolism; D015842:Serine Proteinase Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism
D003911:Dextrans; D004983:Ethanolamines; D015842:Serine Proteinase Inhibitors; C025614:sephadex; D012697:Serine Endopeptidases; D002918:Chymotrypsin; D014357:Trypsin; C007369:2-diethylaminoethanol; D000645:Ammonium Sulfate