Cell growth and oxygen uptake of Escherichia coli and Pseudomonas aeruginosa are differently effected by the genetically engineered Vitreoscilla hemoglobin gene.
Vitreoscilla hemoglobin is a good oxygen trapping agent and its presence in genetically engineered Escherichia coli helps this bacterium to grow better. Here, the potential use of this hemoglobin, for improving the growth and the oxygen transfer properties of Pseudomonas aeruginosa as well as Escherichia coli, was investigated. To stably maintain it in both bacteria, a broad-host range cosmid vector (pHG1), containing the entire coding sequence for Vitreoscilla hemoglobin gene and its native promoter on a 2.3 kb fragment, was constructed. Though at different levels, both bacteria produced hemoglobin and while the oxygen uptake rates of vgb-bearing strains were 2-3-fold greater than that of non-vgb-bearing strains in both bacteria, the growth advantage afforded by the presence of Vitreoscilla hemoglobin was somewhat varied. As an alternative to the traditional method of the improvement of oxygen transfer properties of the environment in which cells are grown, the genetic manipulation applied here improved the oxygen utilization properties of cells themselves.
Geckil
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H
Department of Biological, Chemical, and Physical Sciences, Illinois Institute of Technology, IIT Center, Chicago, IL 60616, USA. hgeckil@inonu.edu.tr
Stark
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BC
Webster
D A
DA
D001426:Bacterial Proteins / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D005798:Genes, Bacterial / Q000235:genetics*; D006454:Hemoglobins / Q000235:genetics*; D010100:Oxygen / Q000378:metabolism*; D010957:Plasmids / Q000138:chemical synthesis / Q000235:genetics; D015202:Protein Engineering / Q000379:methods; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D011995:Recombination, Genetic; D014169:Transformation, Bacterial / Q000235:genetics; D054793:Truncated Hemoglobins
D001426:Bacterial Proteins; D006454:Hemoglobins; D054793:Truncated Hemoglobins; C082613:hemoglobin protein, Vitreoscilla; D010100:Oxygen