title: The DNA form of a retroviroid-like element characterized in cultivated carnation species.
abstract: Carnation small viroid-like RNA (CarSV RNA) is a small (275 nt), circular molecule which is unique among plant viroid-like RNAs in having a tandemly repeated homologous DNA. This DNA form was found fused to DNA sequences of carnation etched ring caulimovirus (CERV) in certain Spanish carnation plants. The observation of a growth abnormality consisting of extensive shoot proliferation in cultivated carnations in Hungary prompted the molecular analysis of these plants, in which both CarSV RNA and DNA forms were detected. Several CarSV DNA sequences were characterized in various Dianthus caryophyllus cultivars which were symptomless or showed different symptoms. CarSV DNA forms showing minor sequence heterogeneities and deletions occurred in the same plant. Unit-length CarSV DNA sequences were proven to accumulate in the plant cell nucleus. The plants studied here were not infected by any of the viruses (including CERV) or other cellular pathogens described previously in carnation.
authors.affiliation: Agricultural Biotechnology Center, Gödöllő, H-2101 PO Box 411, Hungary1.
authors.forename: Krisztina
authors.initials: K
authors.lastname: Hegedűs
authors.affiliation: Agricultural Biotechnology Center, Gödöllő, H-2101 PO Box 411, Hungary1.
authors.forename: László
authors.initials: L
authors.lastname: Palkovics
authors.affiliation: Óbuda Nursery Cooperative, Laboratory of Plant Pathology and Biotechnology, H-1039 Budapest, Királyok útja 226, Hungary2.
authors.forename: Endre Kristóf
authors.initials: EK
authors.lastname: Tóth
authors.affiliation: Agricultural Biotechnology Center, Gödöllő, H-2101 PO Box 411, Hungary1.
authors.forename: Géza
authors.initials: G
authors.lastname: Dallmann
authors.affiliation: Agricultural Biotechnology Center, Gödöllő, H-2101 PO Box 411, Hungary1.
authors.forename: Ervin
authors.initials: E
authors.lastname: Balázs
mesh_terms: D001483:Base Sequence; D004279:DNA, Viral; D019684:Magnoliopsida / Q000821:virology*; D008969:Molecular Sequence Data; D012367:RNA, Viral; D014772:Viroids / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral