Cytotoxicity of fluoride on human pulp cell cultures in vitro.
OBJECTIVE
Numerous studies have revealed that conventional glass-ionomer cements might release fluoride into an aqueous environment. The objective of this study was to examine the effects of fluoride on human pulp cells in vitro.


METHODS
H33258 fluorescence, cell proliferation, protein synthesis, and mitochondrial activity assay were used to investigate the pathobiological effects of fluoride on cultured human pulp cells.


RESULTS
Fluoride was found to be a cytotoxic agent to cultured human pulp cells by inhibiting cell growth, proliferation, mitochondrial activity, and protein synthesis.


CONCLUSIONS
Fluoride release has significant potential for pulpal toxicity.
Chang
Y C
YC
School of Dentistry, Chung Shan Medical and Dental College, Department of Dentistry, Chung Shan Medical and Dental College Hospital, Taichung, Taiwan, ROC.
Chou
M Y
MY
D000704:Analysis of Variance; D006690:Bisbenzimidazole; D002327:Cariostatic Agents / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003124:Colorimetry; D004396:Coloring Agents; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005456:Fluorescent Dyes; D005459:Fluorides / Q000633:toxicity*; D006801:Humans; D007930:Leucine / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects; D014176:Protein Biosynthesis; D011506:Proteins / Q000187:drug effects; D019275:Radiopharmaceuticals; D015203:Reproducibility of Results; D013223:Statistics as Topic; D013778:Tetrazolium Salts; D013844:Thiazoles; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D002327:Cariostatic Agents; D004396:Coloring Agents; D005456:Fluorescent Dyes; D011506:Proteins; D019275:Radiopharmaceuticals; D013778:Tetrazolium Salts; D013844:Thiazoles; D014316:Tritium; C022616:thiazolyl blue; D007930:Leucine; D006690:Bisbenzimidazole; D005459:Fluorides; D013936:Thymidine