The 70-kDa major DNA-compacting protein of the chloroplast nucleoid is sulfite reductase.
The chloroplast nucleoid is a complex of chloroplast DNA and various, mostly uncharacterized proteins. An abundant 70-kDa protein of the isolated nucleoids of pea chloroplasts was identified as sulfite reductase by N-terminal sequence analysis as well as immunoblot analysis, spectrophotometry and enzyme activity analysis. Recombinant maize sulfite reductase was indeed able to compact chloroplast DNA and to form nucleoid-like particles in vitro. The role of sulfite reductase in the structural organization of the nucleoid is discussed.
Sato
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N
Department of Molecular Biology, Saitama University, Urawa, Saitama Prefecture, Japan. naokisat@molbiol.saitama-u.ac.jp
Nakayama
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M
Hase
T
T
D000595:Amino Acid Sequence; D002736:Chloroplasts / Q000201:enzymology* / Q000235:genetics; D018742:DNA, Chloroplast / Q000737:chemistry / Q000378:metabolism; D018744:DNA, Plant / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D018532:Pisum sativum / Q000201:enzymology / Q000235:genetics; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D018742:DNA, Chloroplast; D018744:DNA, Plant; D010940:Plant Proteins; D011994:Recombinant Proteins; D050862:Oxidoreductases Acting on Sulfur Group Donors