title: TUP1 utilizes histone H3/H2B-specific HDA1 deacetylase to repress gene activity in yeast.
abstract: TUP1 is recruited to and represses genes that regulate mating, glucose and oxygen use, stress response, and DNA damage. It is shown here that disruption of either TUP1 or histone deacetylase HDA1 causes histone H3/H2B--specific hyperacetylation next to the TUP1 binding site at the stress-responsive ENA1 promoter. It is also shown that TUP1 interacts with HDA1 in vitro. These data indicate that TUP1 mediates localized histone deacetylation through HDA1. Interestingly, RPD3 deacetylates the ENA1 coding region, and both deacetylases contribute to ENA1 repression. However, epistasis analysis argues that only HDA1 and TUP1 are likely to function in the same pathway. These data define gene and histone targets of HDA1 and illustrate the role of histone deacetylation in TUP1 repression.
authors.affiliation: Department of Biological Chemistry, UCLA School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.
authors.forename: J
authors.initials: J
authors.lastname: Wu
authors.forename: N
authors.initials: N
authors.lastname: Suka
authors.forename: M
authors.initials: M
authors.lastname: Carlson
authors.forename: M
authors.initials: M
authors.lastname: Grunstein
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism; D000107:Acetylation; D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism; D000942:Antigens, Bacterial; D000946:Antigens, Fungal; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D027682:Cation Transport Proteins; D003574:Cytochrome c Group / Q000235:genetics / Q000378:metabolism; D045304:Cytochromes c; D004338:Drug Combinations; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D006023:Glycoproteins; D006655:Histone Deacetylases / Q000235:genetics / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D007763:Lac Operon; D016296:Mutagenesis / Q000502:physiology; D009687:Nuclear Proteins; D011401:Promoter Regions, Genetic / Q000502:physiology; D012097:Repressor Proteins; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D012697:Serine Endopeptidases / Q000235:genetics / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase; D013489:Suppression, Genetic / Q000502:physiology*; D013849:Thimerosal; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D000099160:LexA Repressor Protein
chemical_list: D018528:ATP-Binding Cassette Transporters; D000251:Adenosine Triphosphatases; D000942:Antigens, Bacterial; D000946:Antigens, Fungal; D001426:Bacterial Proteins; D027682:Cation Transport Proteins; D003574:Cytochrome c Group; D045304:Cytochromes c; D004338:Drug Combinations; D005656:Fungal Proteins; D006023:Glycoproteins; D006655:Histone Deacetylases; D006657:Histones; D009687:Nuclear Proteins; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D012697:Serine Endopeptidases; D000254:Sodium-Potassium-Exchanging ATPase; D013849:Thimerosal; D014157:Transcription Factors; D000099160:LexA Repressor Protein; C014059:CYC1 protein, S cerevisiae; C086375:ENA1 protein, S cerevisiae; C489277:STE6 protein, S cerevisiae; C066502:TUP1 protein, S cerevisiae; C011874:imudon; C071665:RPD3 protein, S cerevisiae