title: Stimulation of taxol production and excretion in Taxus spp cell cultures by rare earth chemical lanthanum.
abstract: The trivalent ion of a rare earth element, lanthanum, was tested for elicitor-like effects on taxol production in suspension cultures of four different Taxus spp cells. In T. yunnanensis cell cultures, the lanthanum ion at concentrations from 1.15 to 23.0 microM stimulated taxol production. The lanthanum ion also promoted taxol excretion by the T. yunnanensis cells considerably. The maximum stimulation of taxol production was achieved by the addition of 5.8 microM La3+ to the culture during mid-log growth phase, increasing the volumetric taxol yield by nearly threefold, from 2.61+/-0.37 to 9.89+/-1.92 mg l(-1) over a 28 day culture period. At higher concentrations, i.e. 23.1 and 46.2 microM, however, the lanthanum ion caused significant growth inhibition. For the other three Taxus cell lines, namely an embryo and a leave cell of T. chinensis and a stem cell of T. chinensis marv, the addition of lanthanum ion to the culture only had a significant effect on taxol production by the T. chinensis marv stem cells, increasing the volumetric yield by about threefold to 4.69+/-0.76 mg l(-1). These results suggest that lanthanum has elicitor-like effects on secondary metabolite synthesis of plant cell cultures.
authors.affiliation: Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong. bcjywu@polyu.edu.hk
authors.forename: J
authors.initials: J
authors.lastname: Wu
authors.forename: C
authors.initials: C
authors.lastname: Wang
authors.forename: X
authors.initials: X
authors.lastname: Mei
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D006133:Growth Substances / Q000494:pharmacology; D007811:Lanthanum / Q000494:pharmacology*; D017239:Paclitaxel / Q000096:biosynthesis* / Q000302:isolation & purification*; D010946:Plants, Medicinal; D020946:Taxus / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D014197:Trees
chemical_list: D006133:Growth Substances; D007811:Lanthanum; D017239:Paclitaxel