[Studies on the regulation of glucoamylase gene (glaA) expression in A. niger I. The overall analysis and comparison of the expression of glucoamylase in the overproducing strain A. niger T21 and its original strain A. niger 3.795].
Expression of the A. niger T21 and 3.795 glaA gene was investigated with respect to the growth of mycelia, producing of glucoamylase, copy number of glaA gene as well as glucoamylase mRNA level and its stability. Both A. niger T21 and 3.795 produced substantial glucoamylase at the stable phase when their biomass were the same. After incubating for 72 hours, while the biomass remained the same, the glucoamylase produced by T21 was 10-17 times higher than that by 3.795. Northern analysis showed that in the total RNA from T21 4.3-4.4 fold as much glucoamylase mRNA as from 3.795 was determined. This result along with results with regard to copy number of glaA gene and stability of glucoamylase mRNA supported the suggestion that the increased mRNA level of glucoamylase was contributed by the increasing at transcription level. The inconsistancy between the difference of enzyme production (10-17 fold) and that of the mRNA level (4.3-4.4 fold) revealed that some difference at translation exist probably. Furthermore, expression of both T21 and 3.795 glaA gene was regulated by carbon sources, this regulation occurred at transcription level in the same manner and also probably occurred at translation level.
Qiao
D
D
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080.
Tang
G
G
Zhong
L
L
Xi
Q
Q
Yang
K
K
D001234:Aspergillus niger / Q000378:metabolism*; D005786:Gene Expression Regulation; D005087:Glucan 1,4-alpha-Glucosidase / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
D012333:RNA, Messenger; D005087:Glucan 1,4-alpha-Glucosidase