[Study of activated protein C resistance(APC-R) and FV Leiden in healthy blood donors and patients with thrombosis].
OBJECTIVE
To study activated protein C resistance (APC-R) and FV Leiden in Chinese and their role in the pathogenesis of thrombophilia.


METHODS
APC-R was detected by activated partial thromboplastin time (APTT) in the presence and absence of APC. FV Leiden mutation was detected by PCR amplification with sequence-specific primers and restriction enzyme digestion. The subjects included 32 healthy blood donors (control), 38 patients with thrombosis in Wuhan, China, 106 patients with thrombosis in Homburg, Germany. FV Leiden mutation was screened in 12 patients in Wuhan and 106 in Homburg.


RESULTS
There was no significant difference for APC-R between healthy controls and the patients in Wuhan. No FV Leiden mutation was found in 12 patients in Wuhan. The APC-R frequency of the patients in Homburg was significantly higher than those in Wuhan (22.6% and 7.9%, respectively). Eleven patients in Hombery had FV Leiden mutation.


CONCLUSIONS
APC-R and FV Leiden mutation varied in different regions and races. FV Leiden may not be the main cause of thrombophilia in Chinese.
Hong
M
M
Department of Hematology, Union Hospital, Tongji Medical University, Wuhan 430022.
Wei
W
W
Song
S
S
D020016:Activated Protein C Resistance / Q000235:genetics*; D000293:Adolescent; D000328:Adult; D000368:Aged; D044466:Asian People; D005165:Factor V / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D017354:Point Mutation; D013927:Thrombosis / Q000235:genetics*
C095381:factor V Leiden; D005165:Factor V