[Nucleotide sequence of the Rhizobium huakuii common nodution genes nodA and nodBC].
An another clone pRaN109 which contains nod genes was isolated and identified from the clone bank of R. huakuii 159 DNA using the 32P-labeled 2.3 kb R. meliloti nod DNA fragment as a hybridization probe. Analysis of homologous DNA-DNA hybridization and nucleotide sequence indicated that the 9 kb EcoRI fragment of pRaN109 DNA carried the nodD1BC genes and the 18 kb EcoRI fragment of pRaN109 DNA contained nodD2A genes. The common nodA gene was separated by 6.7 kb from the nodBC genes. Both the nodA and nodB-nodC cistrons showed nod box upstream of these genes. Nucleotide sequence analysis of the genes describes a obviously different organization of common nod genes in R. huakuii 159 compared with that reported for most of the strains from different genera described up to now.
Shen
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Shanghai Institute of Plant Physiology, Chinese Academy of Sciences, Shanghai 200032.
Gong
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Jin
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D000217:Acyltransferases / Q000235:genetics*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics*; D012231:Rhizobium / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D000217:Acyltransferases; C060770:NodA protein, Rhizobiales; D017351:N-Acetylglucosaminyltransferases; C060771:NodC protein, Rhizobiales