title: Localization of two major GABA(A) receptor subunits in the dentate gyrus of the rat and cell type-specific up-regulation following entorhinal cortex lesion.
abstract: GABA(A) receptor subunits show a specific regional distribution in the CNS during development and in the adult animal. In the hippocampal formation, individual subsets of GABAergic interneurons are highly immunoreactive for the alpha1-subunit, whereas granule and pyramidal cells show a strong expression of the alpha2-subunit. Using confocal microscopy and digital image analysis, we demonstrate that in the dentate gyrus the alpha1-subunit immunolabeling appears in differently sized clusters. The large clusters, which are confined to dendrites of interneurons, show no alpha2 labeling, whereas the smaller ones coincide with alpha2-subunit-positive clusters. In the molecular layer, the clusters of both alpha-subunits co-localize with the anchoring protein gephyrin. In the granule cell layer and hilus, we found alpha1- and alpha2-subunit-positive clusters which were devoid of gephyrin labeling. Lesions of the medial entorhinal cortex led to the deafferentation of dendrites in the middle molecular layer of the dentate gyrus. This resulted in a significantly increased concentration of alpha2-subunit-positive clusters. We also observed an increase of alpha1-subunit immunolabeling in the deafferented area. We found no change in the co-localization between alpha1 and alpha2, and no significant change in the number of large alpha1-positive clusters along individual dendritic segments of interneurons. In a previous study, we demonstrated that calbindin-immunoreactive dendrites of granule cells revealed a significant increase in gephyrin immunoreactivity following lesion, whereas parvalbumin-positive dendrites showed no such alterations. The predominant localization of small gephyrin clusters in dendrites of granule cells, which was also described in this study, leads to the conclusion that the increase of the alpha2-subunit-positive clusters, demonstrated in the present study, indicates that, following entorhinal cortex lesion, new GABAergic synapses may be formed and that they contact predominantly granule cell dendrites.
authors.affiliation: Institute of Anatomy, Humboldt University Berlin (Charité), 10098, Berlin, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Simbürger
authors.forename: M
authors.initials: M
authors.lastname: Plaschke
authors.forename: J M
authors.initials: JM
authors.lastname: Fritschy
authors.forename: R
authors.initials: R
authors.lastname: Nitsch
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000032:analysis; D003714:Denervation; D018891:Dentate Gyrus / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism*; D018728:Entorhinal Cortex / Q000166:cytology / Q000473:pathology*; D007150:Immunohistochemistry; D008297:Male; D008565:Membrane Proteins / Q000032:analysis; D018613:Microscopy, Confocal; D009434:Neural Pathways; D010320:Parvalbumins / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D011963:Receptors, GABA-A / Q000032:analysis* / Q000378:metabolism*; D013569:Synapses / Q000737:chemistry / Q000378:metabolism; D015854:Up-Regulation / Q000502:physiology
chemical_list: D002352:Carrier Proteins; D008565:Membrane Proteins; D010320:Parvalbumins; D011963:Receptors, GABA-A; C073441:gephyrin