A comparison of the internal transcribed spacer of the ribosomal DNA for Eubothrium crassum and Eubothrium salvelini (Cestoda: Pseudophyllidea), parasites of salmonid fish.
The internal transcribed spacers (ITS-1 and ITS-2) and 5.8S subunit of the ribosomal RNA gene of Eubothrium crassum and Eubothrium salvelini, cestode parasites of salmonid fish, were sequenced and compared. The lengths and GC contents of the regions sequenced were 1119 bp and 50.1% for E. crassum, and 1117 bp and 50.5% for E. salvelini, respectively. Sequence alignment and comparison of both taxa showed 97.9% similarity. Within the ITS-1 and ITS-2 of both species, the restriction enzymes MspI and Bsh1236I possessed different cleavage sites and could be employed for rapid differentiation of both species of Eubothrium by PCR-RFLP.
Král'ová
I
I
Parasitological Institute, Slovak Academy of Sciences, Hlinkova 3, 040 01, Kosice, Slovak Republic. kralova@saske.sk
Hanzelová
V
V
Scholz
T
T
Gerdeaux
D
D
Spakulová
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002589:Cestoda / Q000145:classification / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics*; D019003:Deoxyribonuclease HpaII / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D015183:Restriction Mapping / Q000662:veterinary; D012487:Salmonidae / Q000469:parasitology*; D016415:Sequence Alignment / Q000662:veterinary
D021903:DNA, Ribosomal Spacer; D012340:RNA, Ribosomal, 5.8S; D019003:Deoxyribonuclease HpaII