Mumps vaccine virus genome is present in throat swabs obtained from uncomplicated healthy recipients.
Seven children were followed for up to 42 days post-vaccination with live mumps vaccine and 37 throat swabs were obtained serially. Viral genomic RNA was detected by reverse transcription-polymerase chain reaction (RT-PCR) in the phosphoprotein (P) and hemagglutinin-neuraminidase (HN) regions. Virus isolation was also attempted. Genomic differentiation of detected mumps virus genome was performed by sequence analysis and/or restriction fragment length polymorphism (RFLP). No adverse reaction was observed in these children. Although mumps virus was not isolated from any of the samples, viral RNA was detected in four samples from three vaccine recipients, 18, 18 and 26, and 7 days after vaccination, respectively. Detected viral RNA was identified as the vaccine strain. Our data suggests that vaccine virus inoculated replicates in the parotid glands but the incidence of virus transmission from recipients to other susceptible subjects should be low.
Nagai
T
T
Nagai Pediatric Clinic, 2-20 Akane-cho, 760-0002, Takamatsu, Japan. t-nagai@mail.netwave.or.jp
Nakayama
T
T
D002675:Child, Preschool; D005260:Female; D016679:Genome, Viral; D006801:Humans; D008297:Male; D009108:Mumps Vaccine / Q000008:administration & dosage / Q000235:genetics*; D009109:Mumps virus / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D010306:Parotid Gland / Q000276:immunology / Q000821:virology; D010614:Pharynx / Q000276:immunology / Q000821:virology; D012150:Polymorphism, Restriction Fragment Length; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014779:Virus Replication
D009108:Mumps Vaccine; D012367:RNA, Viral