Identification and characterization of a novel human vanilloid receptor-like protein, VRL-2.
Remarkable progress has been made recently in identifying a new gene family related to the capsaicin (vanilloid) receptor, VR1. Using a combination of in silico analysis of expressed sequence tag (EST) databases and conventional molecular cloning, we have isolated a novel vanilloid-like receptor, which we call VRL-2, from human kidney. The translated gene shares 46% and 43% identity with VR1 and VRL-1, respectively, and maps to chromosome 12q23-24.1, a locus associated with bipolar affective disorder. VRL-2 mRNA was most strongly expressed in the trachea, kidney, and salivary gland. An affinity-purified antibody against a peptide incorporating the COOH terminal of the receptor localized VRL-2 immunolabel in the distal tubules of the kidney, the epithelial linings of both trachea and lung airways, serous cells of submucosal glands, and mononuclear cells. Unlike VR1 and VRL-1, VRL-2 was not detected in cell bodies of dorsal root ganglia (DRG) or sensory nerve fibers. However, VRL-2 was found on sympathetic and parasympathetic nerve fibers, such as those innervating the arrector pili smooth muscle in skin, sweat glands, intestine, and blood vessels. At least four vanilloid receptor-like genes exist, the newest member, VRL-2 is found in airway and kidney epithelia and in the autonomic nervous system.
Delany
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Genome Informatics and Analysis, Virology and Vaccine Systems, Ion Channel Section, Molecular Recognition, Molecular Genetics, Glaxo Wellcome Research and Development, Medicines Research Centre, Stevenage, Hertfordshire SG1 2NY, United Kingdom.
Hurle
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Facer
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Alnadaf
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T
Plumpton
C
C
Kinghorn
I
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See
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Costigan
M
M
Anand
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Woolf
C J
CJ
Crowther
D
D
Sanseau
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Tate
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D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D027682:Cation Transport Proteins; D002460:Cell Line; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12 / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D007473:Ion Channels; D008297:Male; D008969:Molecular Sequence Data; D012313:RNA / Q000235:genetics / Q000378:metabolism; D021101:Radiation Hybrid Mapping; D051381:Rats; D011955:Receptors, Drug / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D050916:TRPV Cation Channels; D014018:Tissue Distribution
D027682:Cation Transport Proteins; D018076:DNA, Complementary; D007473:Ion Channels; D011955:Receptors, Drug; D050916:TRPV Cation Channels; C416986:TRPV4 protein, human; C486577:Trpv4 protein, rat; D012313:RNA