title: Efficacy of a new collection procedure for preventing bacterial contamination of whole-blood donations.
abstract: BACKGROUND
  Transfusion-related bacterial contamination is a serious problem. The introduction of bacteria into donations at the collection stage seems frequent, despite well-conducted phlebotomy site preparation. Additional preventive measures are required.
  
  
  METHODS
  The aim of this study was to assess the potential efficacy of excluding the first 15 mL of blood to reduce the bacterial contamination of donations. A special device allowed the aseptic collection of two samples at the beginning of donation: S1 (first 15 mL) and S2 (next 15 mL). Bacteriologic cultures of S1 and S2 were performed by using an automated system. The procedure's efficacy was measured by the proportion of positive donations in S1 that were then negative in S2.
  
  
  RESULTS
  S1 and/or S2 were positive in 76 (2.2%) of 3385 donations. In about three-fourths of the culture-positive donations, contamination was detected in the first 15-mL sample only. Gram-positive cocci accounted for 81 percent of species, gram-positive bacilli for 14 percent, and gram-negative bacilli for 5 percent. The new procedure would have prevented the introduction of bacteria in 55 donations, reducing to 0.6 percent the risk of contamination from the first 15 mL collected.
  
  
  CONCLUSIONS
  Although the final effect on blood component bacterial contamination rates cannot be derived from the study, excluding the first 15 mL of blood may reduce the rate of bacterial contamination in donations.
authors.affiliation: French National Blood Service (Etablissement Français du Sang, EFS) Centre-Atlantique and the Faculty of Medicine, University of Tours, Tours, France. Cecile.Bruneau@efs.sante.fr
authors.forename: C
authors.initials: C
authors.lastname: Bruneau
authors.forename: P
authors.initials: P
authors.lastname: Perez
authors.forename: M
authors.initials: M
authors.lastname: Chassaigne
authors.forename: P
authors.initials: P
authors.lastname: Allouch
authors.forename: A
authors.initials: A
authors.lastname: Audurier
authors.forename: C
authors.initials: C
authors.lastname: Gulian
authors.forename: G
authors.initials: G
authors.lastname: Janus
authors.forename: G
authors.initials: G
authors.lastname: Boulard
authors.forename: P
authors.initials: P
authors.lastname: De Micco
authors.forename: L R
authors.initials: LR
authors.lastname: Salmi
authors.forename: L
authors.initials: L
authors.lastname: Noel
mesh_terms: D000328:Adult; D001769:Blood / Q000382:microbiology*; D001782:Blood Donors; D001800:Blood Specimen Collection / Q000379:methods* / Q000592:standards*; D005260:Female; D006801:Humans; D008297:Male