Expression of syndecan-1 is common in human lung cancers independent of expression of epidermal growth factor receptor.
In order to determine syndecan-1 expression in lung cancer, we examined 115 lung cancer specimens and 17 lung cancer cell lines. Syndecan-1 was immunohistochemically stained with a polyclonal antibody in 115 paraffin-embedded specimens; 84 cases out of 97 non-small cell lung cancer (NSCLC) and eight cases out of 18 small cell lung cancer (SCLC) were positively stained. Simultaneously, epidermal growth-factor receptor (EGFR) was stained; 47 cases out of 97 NSCLC and one case of 18 SCLC were positively stained. No significant correlation was shown between EGFR and syndecan-1 expression (P=0.68). Syndecan-1 mRNA was detectable in 16 of 17 lung cancer cell lines and EGFR mRNA in nine of 17. Eight cell lines had syndecan-1 mRNA as well as EGFR mRNA. PR-39 (1 microM) and 80 pM transforming growth factor-beta(1) (TGF-beta(1)), did not increase expressions of syndecan-1 mRNA and EGFR in five lung cancer cell lines. We concluded that lung cancer had detectable syndecan-1; however, expression of syndecan-1 protein did not correlate with survival time of lung cancer patients.
Toyoshima
E
E
First Department of Medicine Asahikawa Medical College, Midorigaoka Higashi 2-1-1-1, Asahikawa 078-8510, Japan.
Ohsaki
Y
Y
Nishigaki
Y
Y
Fujimoto
Y
Y
Kohgo
Y
Y
Kikuchi
K
K
D000368:Aged; D000906:Antibodies; D002289:Carcinoma, Non-Small-Cell Lung / Q000503:physiopathology*; D018288:Carcinoma, Small Cell / Q000503:physiopathology*; D066246:ErbB Receptors / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000503:physiopathology*; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D008875:Middle Aged; D011379:Prognosis; D011509:Proteoglycans / Q000096:biosynthesis*; D016019:Survival Analysis; D053668:Syndecan-1; D053667:Syndecans; D014407:Tumor Cells, Cultured
D000906:Antibodies; D008562:Membrane Glycoproteins; D011509:Proteoglycans; C508241:SDC1 protein, human; D053668:Syndecan-1; D053667:Syndecans; D066246:ErbB Receptors