Isolation of a novel actin-related gene expressed in low-metastatic PC-14 human lung adenocarcinoma.
In this article, we report the identification of differentially expressed genes associated with the metastatic potential of PC-14 human lung adenocarcinoma. The mRNA differential display method was applied to compare mRNAs from six cloned PC-14 cell lines representing different metastatic potentials. A novel gene was identified as being expressed in low-metastatic cells but not in high-metastatic cells. Sequence analysis revealed that this novel gene has an open reading frame of 210 amino acid residues showing 89% amino acid identity with human Arp3, which is one of the actin-related proteins. This gene was designated the Arp11 gene (the 11th Actin-related gene). The Arp11 gene was mapped to human chromosome 7q32-36. Southern blot and PCR analyses show that all of the high-metastatic variant cells have not rearrangements and deletions of Arp11 allele. Expression of the Arp11 gene may regulate the metastatic potential of PC-14 human lung adenocarcinoma.
Shindo-Okada
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N
Biology Division, National Cancer Center Research Institute, 5-1-1, Tsukiji, Chuo-ku, Tokyo, 104-0045, Japan. nokada@gan2.res.ncc.go.jp
Shimizu
K
K
D051378:Actin-Related Protein 3; D000199:Actins / Q000737:chemistry / Q000235:genetics*; D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000595:Amino Acid Sequence; D001483:Base Sequence; D003598:Cytoskeletal Proteins; D020869:Gene Expression Profiling; D015723:Gene Library; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
C495928:ACTR3 protein, human; C422070:ACTR3B protein, human; D051378:Actin-Related Protein 3; D000199:Actins; D003598:Cytoskeletal Proteins; D012333:RNA, Messenger