Occludin and claudin-1 concentrate in the midbody of immortalized mouse hepatocytes during cell division.
It has been believed that epithelial cells maintain tight junctions at all times, including during cell division, to provide a continuous epithelial seal. However, changes in localization of integral tight junction proteins during cell division have not been examined. In this study, using SV40-immortalized mouse hepatocytes transfected with human Cx32 cDNA, in which tight junction strands and the endogenous tight junction proteins occludin, claudin-1, ZO-1, and ZO-2 were induced, we examined changes in localization of the tight junction proteins at all stages of cell division. All tight junction proteins were present between mitotic cells and neighboring cells throughout cell division. In late telophase, the integral tight junction proteins occludin and claudin-1, but not the cytoplasmic proteins ZO-1 and ZO-2, were concentrated in the midbody between the daughter cells and were observed at cell borders between the daugher and neighboring cells. These results indicate that the integral tight junction proteins are regulated in a different manner from the cytoplasmic proteins ZO-1 and ZO-2 during cytokinesis.
Kojima
T
T
Department of Pathology, Cancer Research Institute, Sapporo Medical University School of Medicine, Sapporo, Japan. ktakeshi@sapmed.ac.jp
Kokai
Y
Y
Chiba
H
H
Osanai
M
M
Kuwahara
K
K
Mori
M
M
Mochizuki
Y
Y
Sawada
N
N
D000818:Animals; D062445:Claudin-1; D017630:Connexins / Q000235:genetics / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D008856:Microscopy, Fluorescence; D008938:Mitosis; D062793:Occludin; D010750:Phosphoproteins / Q000378:metabolism; D013692:Telophase; D019108:Tight Junctions / Q000378:metabolism; D014162:Transfection; D062826:Zonula Occludens-1 Protein; D062827:Zonula Occludens-2 Protein; D000097002:Gap Junction beta-1 Protein
D062445:Claudin-1; D017630:Connexins; D018076:DNA, Complementary; D008565:Membrane Proteins; D062793:Occludin; D010750:Phosphoproteins; D062826:Zonula Occludens-1 Protein; D062827:Zonula Occludens-2 Protein; D000097002:Gap Junction beta-1 Protein; C568108:CLDN1 protein, human; C568113:Cldn1 protein, mouse; C568985:OCLN protein, human; C568986:Ocln protein, mouse; C569042:TJP1 protein, human; C569046:TJP2 protein, human; C569043:Tjp1 protein, mouse; C569047:Tjp2 protein, mouse