Expression of a CIC chloride channel in Caenorhabditis elegans gamma-aminobutyric acid-ergic neurons.
In this study, we report the complete cDNA sequence, genomic organization and expression pattern of the Caenorhabditis elegans ClC chloride channel gene, clh-6. Two different types of reporter gene fusions suggest that clh-6 expression is restricted to two gamma-aminobutyric acid-ergic RME neurons. These results are in striking contrast with the wide tissue distribution of messenger RNA for the related mammalian isoforms, CIC-6 and CIC-7. The restricted expression pattern of clh-6 provides a unique opportunity to study the biological function of a neuronal CIC chloride channel.
Bianchi
L
L
Department of Medicine, Division of Genetic Medicine, Vanderbilt University School of Medicine, 451 Preston Research Building, Nashville TN 37232-6304, USA.
Miller
D M
DM
George
A L
AL
D000595:Amino Acid Sequence / Q000235:genetics; D000818:Animals; D017173:Caenorhabditis elegans / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins; D002232:Carbocyanines / Q000494:pharmacology; D018118:Chloride Channels / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D005456:Fluorescent Dyes / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000502:physiology*; D049452:Green Fluorescent Proteins; D007202:Indicators and Reagents / Q000032:analysis; D008164:Luminescent Proteins / Q000032:analysis; D008969:Molecular Sequence Data; D009420:Nervous System / Q000166:cytology / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D010802:Phylogeny; D020033:Protein Isoforms / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
C035005:3,3'-dihexadecylindocarbocyanine; D029742:Caenorhabditis elegans Proteins; D002232:Carbocyanines; D018118:Chloride Channels; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D008164:Luminescent Proteins; D020033:Protein Isoforms; C490799:clh-6 protein, C elegans; D049452:Green Fluorescent Proteins; D005680:gamma-Aminobutyric Acid