Minor role of oxidative stress during intermediate phase of acute pancreatitis in rats.
Reactive oxygen species have been implicated in the pathogenesis of acute pancreatitis. Few studies have focused on the loss of endogenous antioxidants and molecular oxidative damage. Two acute pancreatitis models in rats; taurocholate (3% intraductal infusion) and cerulein (10 microg/kg/h), were used to study markers of oxidative stress: Glutathione, ascorbic acid, and their oxidized forms (glutathione disulfide and dehydroascorbic acid), malondialdehyde, and 4-hydroxynoneal in plasma and pancreas, as well as 7-hydro-8-oxo-2'-deoxyguanosine in pancreas. In both models, pancreatic glutathione depleted by 36-46% and pancreatic ascorbic acid depleted by 36-40% (p <.05). In the taurocholate model, plasma glutathione was depleted by 34% (p <.05), but there were no significant changes in plasma ascorbic acid or in plasma and pancreas dehydroascorbic acid, malondialdehyde, and 4-hydroxynoneal, and no significant changes in the pancreas glutathione disulfide/glutathione ratio. While pancreas glutathione disulfide/glutathione ratio increased in the cerulein model, there were no significant changes in plasma glutathione, plasma, or pancreas ascorbic acid, dehydroascorbic acid, 4-hydroxynoneal, and malondialdehyde, or in pancreas 7-hydro-8-oxo-2'-deoxyguanosine. Reactive oxygen species have a minor role in the intermediate stages of pancreatitis models.
Kruse
P
P
Department of Pharmacology, University of Copenhagen, Stavangergade 3, 3.th.DK-2100 Copenhagen, Denmark. peterkruse@dadlnet.dk
Anderson
M E
ME
Loft
S
S
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000208:Acute Disease; D000447:Aldehydes / Q000097:blood / Q000378:metabolism; D000818:Animals; D001205:Ascorbic Acid / Q000097:blood / Q000378:metabolism; D015415:Biomarkers; D002108:Ceruletide; D003683:Dehydroascorbic Acid / Q000097:blood / Q000378:metabolism; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D005978:Glutathione / Q000097:blood / Q000378:metabolism; D019803:Glutathione Disulfide / Q000097:blood / Q000652:urine; D008297:Male; D008315:Malondialdehyde / Q000097:blood / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010195:Pancreatitis / Q000209:etiology*; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D013656:Taurocholic Acid
D000447:Aldehydes; D015415:Biomarkers; D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D013656:Taurocholic Acid; D000080242:8-Hydroxy-2'-Deoxyguanosine; D002108:Ceruletide; D003849:Deoxyguanosine; D005978:Glutathione; C027576:4-hydroxy-2-nonenal; D001205:Ascorbic Acid; D019803:Glutathione Disulfide; D003683:Dehydroascorbic Acid