[Sequencing the 16S rDNA of representative strain of new rhizobial group and determining of its phylogenetic relationship].
The full-length 16S rDNA sequence of representative strain SH2672 were sequenced by the dideoxy-mediated chain-termination method. This sequence was compared with that of type strains of all known rhizobia and related bacteria. An unrooted phylogenetic tree was produced. In this tree, strain SH2672, Mesorhizobium loti, M. huakuii, M. tianshanense, M. mediterraneum, and M. ciceri constituted a branch. Within this branch, the similarity values of 16S rDNA sequences between strain SH2672 and M. loti, M. huakuii, M. tianshanense, M. mediterraneum and M. ciceri were 96.3%, 96.4%, 97.2%, 95.1% and 95.6% respectively. All similarity values was more than 95%, this indicated that these species should belong to the same genus. The values of DNA-DNA homology between type strains of these species were less than 70%, which showed that strain SH2672 (representative strain of new group) represented a new rhizobial species.
Tan
Z
Z
College of Biological Science, China Agricultural University, Beijing 100094.
Chen
W
W
D001483:Base Sequence; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012231:Rhizobium / Q000145:classification / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S