title: An unusual desaturase in Aquilegia vulgaris.
abstract: Aquilegia vulgaris seed oil contains high levels of the rare fatty acid columbinic acid (18:3 Delta(5,9,12)), which is unusual in having the double bond at the Delta(5) carbon in the trans configuration. Columbinic acid was found to be a seed-specific fatty acid not only present in the storage oil but also in membrane lipids. Several putative gene fragments have been isolated from plant RNA with sequences similar to previously characterized 'front-end' desaturases. Functional characterization of the Aquilegia cDNA is underway.
authors.affiliation: School of Biological Sciences, University of Bristol, Woodland Road, Bristol BS8 1UG, UK.
authors.forename: A J
authors.initials: AJ
authors.lastname: Longman
authors.forename: L V
authors.initials: LV
authors.lastname: Michaelson
authors.forename: O
authors.initials: O
authors.lastname: Sayanova
authors.forename: J A
authors.initials: JA
authors.lastname: Napier
authors.forename: A K
authors.initials: AK
authors.lastname: Stobart
mesh_terms: D001665:Binding Sites; D015786:Cytochromes b5 / Q000378:metabolism; D044943:Fatty Acid Desaturases / Q000235:genetics / Q000378:metabolism*; D008042:Linolenic Acids / Q000032:analysis / Q000378:metabolism; D008055:Lipids / Q000737:chemistry*; D008563:Membrane Lipids / Q000737:chemistry; D016366:Open Reading Frames; D010938:Plant Oils / Q000737:chemistry; D010944:Plants / Q000201:enzymology* / Q000235:genetics; D012639:Seeds / Q000737:chemistry
chemical_list: D008042:Linolenic Acids; D008055:Lipids; D008563:Membrane Lipids; D010938:Plant Oils; C034302:5,9,12-octadecatrienoic acid; D015786:Cytochromes b5; D044943:Fatty Acid Desaturases