Patch esophagoplasty using AlloDerm as a tissue scaffold.
OBJECTIVE
Anastomotic leak and stricture are common causes of morbidity after esophageal repair. The authors describe a technique of patch esophagoplasty using decellularized human skin.


METHODS
Twelve conditioned dogs underwent a cervical 2.0- x 1.0-cm esophagoplasty with AlloDerm. A gastrostomy tube was used for feedings until an esophagram was performed on the 10th to 14th postoperative day. Dogs were then given oral chow and followed up for leak and dysphagia. Animals were killed at 1-, 2-, and 3-month intervals and evaluated for stricture, diverticula formation, and patch histology.


RESULTS
All animals survived, and none had sepsis or dysphagia. All esophagrams were without evidence of leak or stricture. At death there were no strictures or diverticula. Histologic examination of 1-month specimens showed partial reepithelialization of the patch with neovascularization. Control staining of AlloDerm was strongly positive for elastin. This was decreased in the region of the patch at 1 month. Two-month specimens showed intact epithelium and an increase in the caliber of new blood vessels. Three-month specimens showed no significant variation from 2-month animals.


CONCLUSIONS
Decellularized human skin (AlloDerm) provides a temporary collagen framework on which esophageal healing can occur and function can be maintained.
Isch
J A
JA
Sections of Pediatric Surgery and Pediatric Pathology, Indiana University School of Medicine and J.W. Riley Hospital for Children, Indianapolis, IN 46202-5200, USA.
Engum
S A
SA
Ruble
C A
CA
Davis
M M
MM
Grosfeld
J L
JL
D000818:Animals; D003094:Collagen; D004285:Dogs; D004940:Esophageal Stenosis / Q000628:therapy; D004944:Esophagoplasty / Q000379:methods*; D006801:Humans; D011184:Postoperative Period; D019651:Plastic Surgery Procedures / Q000379:methods; D016038:Skin Transplantation / Q000379:methods*
D003094:Collagen