Use of arbitrarily primed polymerase chain reaction to investigate Mycoplasma bovis outbreaks.
Colony lineages from three Mycoplasma bovis outbreaks representing different husbandry conditions in the United States were characterized with arbitrarily primed polymerase chain reaction (AP-PCR). Cases studied included a closed beef herd, a dairy calf ranch, and a feedlot. The DNA was obtained from colony lineages and used for AP-PCR with primers REP1R-I and REP2-I. Case A and C lineages were uniform by AP-PCR analysis. Lineages from case B showed heterogeneity with AP-PCR. Outbreaks A and C were therefore both infected by one source, while the ranch (case B) was infected by multiple calf shipments. The AP-PCR typing method provides genotypic epidemiological information to successfully characterize M. bovis from sequential sampling of outbreaks and different husbandry conditions.
Butler
J A
JA
Veterinary Medical Research Institute, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011, USA.
Pinnow
C C
CC
Thomson
J U
JU
Levisohn
S
S
Rosenbusch
R F
RF
D000822:Animal Husbandry; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000382:microbiology*; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004196:Disease Outbreaks; D005260:Female; D008297:Male; D009174:Mycoplasma / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D011019:Pneumonia, Mycoplasma / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D014481:United States / Q000453:epidemiology
D017931:DNA Primers; D004269:DNA, Bacterial