title: Secretion monitor, SecM, undergoes self-translation arrest in the cytosol.
abstract: The product of the Escherichia coli secM gene (secretion monitor, formerly gene X), upstream of secA, is involved in secretion-responsive control of SecA translation. In wild-type cells, SecM is rapidly degraded by the periplasmic tail-specific protease. It is also subject to a transient translation pause at a position close to the C terminus. The elongation arrest was strikingly prolonged when translocation of SecM was impaired. SRP was not required for this arrest. Instead, the nascent SecM product itself may participate, as the arrest was diminished when it incorporated a proline analog, azetidine. We propose that cytosolically localized nascent SecM undergoes self-translation arrest, thereby enhancing translation of secA through an altered secondary structure of the secM-secA messenger RNA.
authors.affiliation: Institute for Virus Research, Kyoto University, Kyoto 606-8507, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Nakatogawa
authors.forename: K
authors.initials: K
authors.lastname: Ito
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics; D000906:Antibodies; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D002352:Carrier Proteins / Q000235:genetics; D002876:Chromosomes, Bacterial / Q000235:genetics; D003600:Cytosol / Q000502:physiology; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D005816:Genetic Complementation Test; D026901:Membrane Transport Proteins; D016296:Mutagenesis / Q000502:physiology; D014176:Protein Biosynthesis / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D000069816:SEC Translocation Channels; D000081416:SecA Proteins
chemical_list: D000906:Antibodies; D001426:Bacterial Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D026901:Membrane Transport Proteins; D012333:RNA, Messenger; D000069816:SEC Translocation Channels; D000251:Adenosine Triphosphatases; D000081416:SecA Proteins