MRNA expression on shape-engineered endothelial cells: adhesion molecules ICAM-1 and VCAM-1.
This study was designed to assess the effect of cell shape on mRNA expression of two adhesion molecules, intracellular adhesion molecule-1 and vascular adhesion molecule-1, on endothelial cells. Photo-microprocessing using photoreactive poly(ethylene glycol) produced two different patterned-cell adhesive regions on tissue culture dishes: one is a striped region on which adhered cells are highly elongated and aligned along the long axis of the striped pattern, and the other is a circular region on which cells are less spread out and rounded. mRNA expressions, measured by the reverse transcription-polymerase chain reaction technique, revealed higher mRNA expression for intracellular adhesion molecule-1 and lower mRNA expression for vascular adhesion molecule-1 on elongated cells than those on round cells. This indicates that surface-induced cell shape induces changes in the mRNA expression of these molecules. The significance of cell-shape-induced mRNA expression is discussed in conjunction with the experimental results of flow-induced expression at molecular and mRNA levels.
Kato
S
S
Department of Bioengineering, National Cardiovascular Center Research Institute, Suita, Osaka, Japan.
Ando
J
J
Matsuda
T
T
D002448:Cell Adhesion; D048429:Cell Size / Q000502:physiology*; D002478:Cells, Cultured; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism* / Q000648:ultrastructure; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000096:biosynthesis; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D011092:Polyethylene Glycols; D012333:RNA, Messenger / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016707:Tissue Fixation; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis*
D012333:RNA, Messenger; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D011092:Polyethylene Glycols; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases