title: Mass spectrometric identification of a candidate biomarker peptide from the in vitro interaction of epichlorohydrin with red blood cells.
abstract: The reaction products of epichlorohydrin with human alpha- and beta- globins, obtained through in vitro incubation of these compounds and red blood cells, were determined by using reversed-phase high-performance liquid chromatography (RP-HPLC), electrospray ionization mass spectrometry and matrix-assisted laser desorption/ionization tandem mass spectrometry. The alpha-globin was much more reactive than the beta-globin. At low incubation ratios, approximating the order of magnitude of epichlorohydrin concentration as found in workplaces, the only modified peptide still detectable was the 62-90 belonging to the alpha-chain and carrying an incremental mass of 92 u on either His72 or His89. Given that the two peptides co-eluted in a single chromatographic peak during RP-HPLC separation, they could be chosen as suitable biomarkers for quantification in the setting up of a new methodology for the biological monitoring of persons occupationally exposed, replacing currently known procedures.
authors.affiliation: Centro Internazionale di Servizi di Spettrometria di Massa, Istituto di Scienze dell'Alimentazione del CNR, Via Roma 52, I-83100 Avellino Italy.
authors.forename: N
authors.initials: N
authors.lastname: Miraglia
authors.forename: G
authors.initials: G
authors.lastname: Pocsfalvi
authors.forename: P
authors.initials: P
authors.lastname: Ferranti
authors.forename: A
authors.initials: A
authors.lastname: Basile
authors.forename: N
authors.initials: N
authors.lastname: Sannolo
authors.forename: A
authors.initials: A
authors.lastname: Acampora
authors.forename: L
authors.initials: L
authors.lastname: Soleo
authors.forename: F
authors.initials: F
authors.lastname: Palmieri
authors.forename: S
authors.initials: S
authors.lastname: Caira
authors.forename: B
authors.initials: B
authors.lastname: De Giulio
authors.forename: A
authors.initials: A
authors.lastname: Malorni
mesh_terms: D000596:Amino Acids / Q000032:analysis; D015415:Biomarkers / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D004811:Epichlorohydrin / Q000097:blood*; D004912:Erythrocytes / Q000737:chemistry*; D005914:Globins / Q000378:metabolism; D006801:Humans; D013058:Mass Spectrometry; D016273:Occupational Exposure; D010446:Peptide Fragments / Q000097:blood; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014357:Trypsin / Q000378:metabolism
chemical_list: D000596:Amino Acids; D015415:Biomarkers; D010446:Peptide Fragments; D004811:Epichlorohydrin; D005914:Globins; D014357:Trypsin