A common deletion at chromosomal region 17q21 in sporadic prostate tumors distal to BRCA1.
Prostate cancer is the most common cancer in males in the United States, yet the etiology of this disease is still poorly understood. In previous work from our laboratory, one or more deleted regions were found in prostate tumors distal to the breast and ovarian cancer susceptibility gene (BRCA1) on chromosome 17. This suggested that genes at 17q21 may play a pivotal role in prostate cancer progression, and there may be new tumor suppressor genes at this locus. We now present a physical map built with P1, P1 artificial chromosome, and bacterial artificial chromosome clones encompassing a DNA sequence anchored by multiple STS markers. The analysis of prostate tumors indicated an 85-kb novel commonly deleted interval flanked by D17S1184-D17S183-D17S1203-D17S1860, which is at least 470 kb distal to the BRCA1 gene. Fifty-four of 126 prostrate cancer cases (43%) showed a deletion by a direct FISH technique using P1 probes in this region. Searching with clone end sequences in the sequence database BLAST, the deleted clone covered genomic DNA sequence that contained upstream binding factor (UBF), EPB3 genes, SHCL1, ASB-4-like sequence, and acidic protein-like sequence. PCR for the ESTs confirmed that these genes or ESTs are within the deletion region. Our results will be helpful for finding candidate tumor suppressor genes in prostate cancer.
Dai
Q
Q
Department of Pediatrics, University of Utah School of Medicine, Salt Lake City, Utah 84112, USA.
Deubler
D A
DA
Maxwell
T M
TM
Zhu
X L
XL
Cui
J
J
Rohr
L R
LR
Stephenson
R A
RA
Brothman
A R
AR
D002872:Chromosome Deletion; D002886:Chromosomes, Human, Pair 17; D020451:Contig Mapping; D004252:DNA Mutational Analysis; D016208:Databases, Factual; D020224:Expressed Sequence Tags; D017353:Gene Deletion; D019398:Genes, BRCA1 / Q000235:genetics*; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008957:Models, Genetic; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D016133:Polymerase Chain Reaction; D011471:Prostatic Neoplasms / Q000235:genetics*; D016324:Sequence Tagged Sites
D005819:Genetic Markers