KIAP, a novel member of the inhibitor of apoptosis protein family.
We have identified a novel human gene, kiap (kidney inhibitor of apoptosis protein) that encodes a single BIR domain and a RING zinc finger domain. kiap has been assigned to the q13.3 region of human chromosome 20 by fluorescent in situ hybridization analysis. Northern blot analysis indicates that KIAP is expressed mainly in placenta, lymph node and fetal kidney. In this report, we show that overexpression of KIAP blocks apoptosis induced by menadione or by overexpression of BAX. In addition, we show that overexpression of KIAP enhances apoptosis induced by etoposide, and, that KIAP fails to block apoptosis induced by overexpression of Fas. Thus, KIAP, a new member of the inhibitor of apoptosis protein (IAP) family, has pleiotropic effects on apoptosis induced by various stimuli.
Lin
J H
JH
Biochemistry and Molecular Biology Graduate Group, University of California, Davis, California, USA. jiing_huey_lin@berlex.com
Deng
G
G
Huang
Q
Q
Morser
J
J
D000595:Amino Acid Sequence; D017209:Apoptosis / Q000502:physiology*; D001426:Bacterial Proteins / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D001483:Base Sequence; D002874:Chromosome Mapping; D002890:Chromosomes, Human, Pair 20; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis; D016208:Databases, Factual; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D019476:Insect Proteins; D007621:Karyotyping; D007668:Kidney / Q000166:cytology / Q000502:physiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D011506:Proteins; D012333:RNA, Messenger / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014764:Viral Proteins / Q000737:chemistry; D019014:fas Receptor / Q000096:biosynthesis / Q000502:physiology
D001426:Bacterial Proteins; D018076:DNA, Complementary; D051034:Inhibitor of Apoptosis Proteins; D019476:Insect Proteins; D011506:Proteins; D012333:RNA, Messenger; D014764:Viral Proteins; D019014:fas Receptor; C086430:inhibitor of apoptosis, Nucleopolyhedrovirus