Collateral projections of nucleus raphe dorsalis neurones to the caudate-putamen and region around the nucleus raphe magnus and nucleus reticularis gigantocellularis pars alpha in the rat.
It was examined whether or not the nucleus raphe dorsalis (RD) neurons projecting to the caudate-putamen (CPu) might also project to the motor-controlling region around the nucleus raphe magnus (NRM) and nucleus reticularis gigantocellularis pars alpha (Gia) in the rat. Single RD neurons projecting to the CPu and NRM/Gia by way of axon collaterals were identified by the retrograde double-labeling method with fluorescent dyes, Fast Blue and Diamidino Yellow, which were injected respectively into the CPu and NRM/Gia. Then, serotonin (5-HT)-like immunoreactivity of the double-labeled RD neurons was examined immunohistochemically; approximately 60% of the double-labeled RD neurons showed 5-HT-like immunoreactivity. The results indicated that some of serotonergic and non-serotonergic RD neurons might control motor functions simultaneously at the levels of the CPu and NRM/Gia by way of axon collaterals.
Li
Y Q
YQ
Department of Anatomy and K.K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an 710032, People's Republic of China.
Kaneko
T
T
Mizuno
N
N
D000578:Amidines / Q000494:pharmacology; D000818:Animals; D001369:Axons / Q000378:metabolism / Q000648:ultrastructure*; D002452:Cell Count; D005456:Fluorescent Dyes / Q000494:pharmacology; D007150:Immunohistochemistry; D008297:Male; D008526:Medulla Oblongata / Q000166:cytology* / Q000378:metabolism; D008636:Mesencephalon / Q000166:cytology* / Q000378:metabolism; D017072:Neostriatum / Q000166:cytology* / Q000378:metabolism; D009434:Neural Pathways / Q000166:cytology* / Q000378:metabolism; D011903:Raphe Nuclei / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012154:Reticular Formation / Q000166:cytology* / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism
D000578:Amidines; D005456:Fluorescent Dyes; C031455:diamidino compound 253-50; D012701:Serotonin; C037945:diamidino yellow