title: Mutations of the Helicobacter pylori genes rdxA and pbp1 cause resistance against metronidazole and amoxicillin.
abstract: To investigate amoxicillin and metronidazole resistance of Helicobacter pylori, we compared putative resistance genes between resistant strains obtained in vitro and their sensitive parent strain. All metronidazole-resistant strains had rdxA mutations, and an amoxicillin-resistant strain had pbp1 and pbp2 mutations. By transforming PCR products of these mutated genes into antibiotic-sensitive strains, we showed that rdxA null mutations were sufficient for metronidazole resistance, while pbp1 mutations contributed to amoxicillin resistance of H. pylori.
authors.affiliation: Yale University, New Haven, Connecticut 06511, USA. Ralf.Paul@Yale.edu
authors.forename: R
authors.initials: R
authors.lastname: Paul
authors.forename: S
authors.initials: S
authors.lastname: Postius
authors.forename: K
authors.initials: K
authors.lastname: Melchers
authors.forename: K P
authors.initials: KP
authors.lastname: Schäfer
mesh_terms: D000595:Amino Acid Sequence; D000658:Amoxicillin / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D002352:Carrier Proteins; D004352:Drug Resistance, Microbial / Q000235:genetics; D016480:Helicobacter pylori / Q000187:drug effects / Q000235:genetics*; D006602:Hexosyltransferases / Q000235:genetics*; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D008795:Metronidazole / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics*; D002267:Muramoylpentapeptide Carboxypeptidase; D009154:Mutation; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002352:Carrier Proteins; D008565:Membrane Proteins; D009097:Multienzyme Complexes; D046915:Penicillin-Binding Proteins; D008795:Metronidazole; C077236:RdxA protein, Rhodobacter sphaeroides; D000658:Amoxicillin; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase