The mtl genes and the mannitol-1-phosphate dehydrogenase from Klebsiella pneumoniae KAY2026.
The mtl operon of Klebsiella pneumoniae KAY2026 (formerly Aerobacter aerogenes 1033-5P14) was shown to contain as the promoter-proximal gene mtlA, encoding a D-mannitol-specific enzyme II transporter (IICBA(Mtl)). This gene is followed by mtlD, coding for a mannitol-1-phosphate dehydrogenase (MtlD, 382 amino acid residues), and mtlR (MtlR, 195 amino acid residues) coding for a putative repressor, gene mtlR overlaps the termination codon of mtlD. The DNA and protein sequences are highly similar to the corresponding genes (81% identical bp) and proteins (79-85% identical amino acids) of Escherichia coli K-12. A truncated form of MtlD lacking the 162 C-terminal amino acid residues still shows 10% dehydrogenase activity which may explain the controversy in the literature concerning the properties of mannitol-phosphate and other medium-length dehydrogenases.
Otte
S
S
Fachbereich Biologie/Chemie, Universität Osnabrück, D-49069, Osnabrück, Germany.
Lengeler
J W
JW
D000595:Amino Acid Sequence; D004269:DNA, Bacterial / Q000032:analysis; D005798:Genes, Bacterial; D007711:Klebsiella pneumoniae / Q000201:enzymology / Q000235:genetics*; D008353:Mannitol / Q000378:metabolism; D008969:Molecular Sequence Data; D009876:Operon; D017434:Protein Structure, Tertiary; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D013401:Sugar Alcohol Dehydrogenases / Q000235:genetics* / Q000378:metabolism
D004269:DNA, Bacterial; D008353:Mannitol; D013401:Sugar Alcohol Dehydrogenases; C021544:mannitol-1-phosphate dehydrogenase