title: High-oleic acid Australian Brassica napus and B. juncea varieties produced by co-suppression of endogenous Delta12-desaturases.
abstract: Genetic engineering methods have been used successfully to modify the fatty acid profile of elite Australian germplasm of Brassica napus and B. juncea. Co-suppression plasmids carrying oleate desaturase genes from each species have been constructed and transferred into Australian elite breeding lines of B. napus and B. juncea using Agrobacterium tumifaciens plant-transformation techniques. Modifications to existing Brassica transformation protocols and the use of an intron-interrupted hygromycin-resistance gene as the selectable marker have resulted in improved transformation efficiencies. Silencing of the endogenous oleate desaturase genes has resulted in substantial increases in oleic acid levels, up to 89% in B. napus and 73% in B. juncea.
authors.affiliation: CSIRO Division of Plant Industry, Canberra, ACT, Australia. peter.stoutjesdijk@pi.csiro.au
authors.forename: P A
authors.initials: PA
authors.lastname: Stoutjesdijk
authors.forename: C
authors.initials: C
authors.lastname: Hurlestone
authors.forename: S P
authors.initials: SP
authors.lastname: Singh
authors.forename: A G
authors.initials: AG
authors.lastname: Green
mesh_terms: D055516:2S Albumins, Plant; D016960:Agrobacterium tumefaciens / Q000235:genetics; D001315:Australia; D001937:Brassica / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D044943:Fatty Acid Desaturases / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D019301:Oleic Acid / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics; D030821:Plants, Genetically Modified / Q000737:chemistry / Q000502:physiology; D010957:Plasmids; D011401:Promoter Regions, Genetic; D013045:Species Specificity; D013489:Suppression, Genetic
chemical_list: D055516:2S Albumins, Plant; D010940:Plant Proteins; D019301:Oleic Acid; C050363:napin protein, Brassica napus; D044943:Fatty Acid Desaturases; C040448:delta-12 fatty acid desaturase