Scale-up of filamentous organisms from tubes and shake-flasks into stirred vessels.
The choice of small-scale fermentation systems contributes significantly to a successful scale-up. Creasing of flasks and the chosen shaker parameters influence the production of secondary metabolites in a strain- and even compound-specific manner. Using actinomycetes and fungi as model organisms the influence of the small-scale fermentation system on the production of various secondary metabolites is described and the effects on screening success and scale-up are considered.
Katzer
W
W
TerraGen Discovery (UK) Ltd., Slough, UK
Blackburn
M
M
Charman
K
K
Martin
S
S
Penn
J
J
Wrigley
S
S