Egg contamination by Salmonella serovar enteritidis following vaccination with Delta-aroA Salmonella serovar typhimurium.
The efficacy of an aroA Salmonella serovar typhimurium modified live vaccine to decrease internal egg contamination after oral challenge of hens with egg-contaminating Salmonella serovar enteritidis was assessed. Challenge was with a mixed phenotype of S. enteritidis that had virulence characteristics previously associated with enhanced oral invasiveness and egg contamination in chickens. Immunized birds had fewer positive ovary/oviduct pools and lower cfu g(-1) cecal contents than did non-immunized birds, but the differences were not significant. The number of positive intestinal (duodenum, jejunum, ileum) and organ (spleen, kidney, liver) pools following challenge from each treatment group were equivalent. Most importantly, immunization did not decrease egg contamination. These results suggest that the ability of modified live vaccines to reduce internal egg contamination by S. serovar enteritidis can be assessed using characterized strains for challenge.
Parker
C
C
United States Department of Agriculture, ARS-SEPRL, Athens, GA 30605, USA.
Asokan
K
K
Guard-Petter
J
J
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D002645:Chickens; D004531:Eggs / Q000382:microbiology*; D005506:Food Contamination; D011201:Poultry Diseases / Q000382:microbiology / Q000517:prevention & control; D012481:Salmonella Infections, Animal / Q000517:prevention & control*; D022562:Salmonella Vaccines / Q000008:administration & dosage*; D012477:Salmonella enteritidis / Q000472:pathogenicity*; D012486:Salmonella typhimurium / Q000276:immunology*; D014436:Typhoid-Paratyphoid Vaccines / Q000008:administration & dosage*; D014611:Vaccination
D000907:Antibodies, Bacterial; D022562:Salmonella Vaccines; D014436:Typhoid-Paratyphoid Vaccines; C087350:aroA Salmonella vaccine