Sodium 1-monolinolenin isolated from Italian ryegrass (Lolium multiflorum Lam) induces apoptosis in human lymphoid leukemia Molt 4B cells.
The exposure of human lymphoid leukemia Molt 4B cells to sodium 1-monolinolenin (SML) which was isolated from the leaves of Italian ryegrass (Lolium multiflorum Lam) and identified by Mass, and (1)H- and (13)C-NMR, led to both growth inhibition and induction of programmed cell death (apoptosis). Morphological change showing apoptotic bodies was observed in the Molt 4B cells treated with SML. The fragmentation by SML of DNA to oligonucleosomal-sized fragments, that is a characteristic of apoptosis, was observed to be both concentration- and time-dependent. These findings suggest that growth inhibition by SML of Molt 4B cells results from the induction of apoptosis in the cells.
Kyohkon
M
M
Faculty of Bioresources, Mie University, Tsu-city, Mie 514-0001, Japan.
Komiya
T
T
Ohwaki
S
S
Katsuzaki
H
H
Imai
K
K
Nishide
R
R
Ikeda
H
H
Hibasami
H
H
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D048429:Cell Size; D053938:DNA Fragmentation; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007945:Leukemia, Lymphoid; D008042:Linolenic Acids / Q000494:pharmacology*; D008129:Lolium / Q000737:chemistry*; D008214:Lymphocytes / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D013997:Time Factors; D014407:Tumor Cells, Cultured
D000972:Antineoplastic Agents, Phytogenic; D008042:Linolenic Acids; C434927:linolenin