title: Synaptic membrane morphology in the rat cerebral cortex during development: image analysis of freeze-etching replicas of isolated synapses and synapses in situ.
abstract: Both synaptic sites in situ from rat cerebral cortex and isolated ones in synaptosomal and growth cone fractions derived from it were studied during postnatal development. Freeze-etching technique and image analysis were used to determine the size of the intramembranous particles in the pre- and postsynaptic sites. At each age investigated, the greatest mean particle size was established on the E-face of the postsynaptic sites and ranges from 6.2 nm (day 0) to nearly 10 nm (day 90). The continuous mean particle size increase from birth to maturity shows the same rate but a different rhythm for the two synaptic sites. The results indicate that fractionation do not disturb the correlation between the particle size and age thus outlining the stability of the developing synaptic membrane morphology to physical treatments.
authors.affiliation: Department of Anatomy and Histology, Medical University, BG-1431, Sofia, Bulgaria. surchev@medfac.acad.bg
authors.forename: L
authors.initials: L
authors.lastname: Surchev
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000254:growth & development* / Q000648:ultrastructure*; D005613:Freeze Etching; D007091:Image Processing, Computer-Assisted; D051381:Rats; D017208:Rats, Wistar; D013569:Synapses / Q000502:physiology / Q000648:ultrastructure*; D013570:Synaptic Membranes / Q000648:ultrastructure*