title: Prolonged recycling of internalized neurotrophins in the nerve terminal.
abstract: BACKGROUND
  Neurons require contact with their target tissue in order to survive and make correct connections. The retrograde axonal transport of neurotrophins occurs after receptor-mediated endocytosis into vesicles at the nerve terminal. However, the mechanism by which the neurotrophin signal is propagated from axon terminal to cell body remains unclear.
  
  
  METHODS
  Retrograde axonal transport was examined using the transport of I(125)-labeled neurotrophins from the eye to sympathetic and sensory ganglia. The phenomena was further studied by adding rhodamine-labeled nerve growth factor (NGF) to cultures of dissociated sympathetic ganglia and the movement of organelles followed with the aid of video microscopy.
  
  
  RESULTS
  I(125)-labeled neurotrophins were transported from the eye to the sympathetic and sensory ganglia. A 100-fold excess of unlabeled neurotrophin, administered up to 4 h after the labeled material, completely prevented accumulation of labeled neurotrophin in the ganglia. The effect was specific for the labeled neurotrophin as administration of a high concentration of a different neurotrophin failed to inhibit the transport. In dissociated cultures, we found rapid binding of label, to surface membrane receptors, followed by an accumulation of labeled vesicles in the growth cone. Incubation of these cultures with unlabeled NGF led to a rapid loss of label in the growth cones.
  
  
  CONCLUSIONS
  These results suggest that there is a pool of internalized neurotrophin, in vesicles in the nerve terminal, which is in rapid equilibrium with the external environment. It is from this pool that a small fraction of the neurotrophin-containing vesicles is targeted for retrograde transport. Potential models for this system are presented.
authors.affiliation: Division of Neuroscience, John Curtin School of Medical Research, Australian National University, Canberra, Australia.
authors.forename: M W
authors.initials: MW
authors.lastname: Weible
authors.forename: S E
authors.initials: SE
authors.lastname: Bartlett
authors.forename: A J
authors.initials: AJ
authors.lastname: Reynolds
authors.forename: I A
authors.initials: IA
authors.lastname: Hendry
mesh_terms: D000818:Animals; D001370:Axonal Transport; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D002478:Cells, Cultured; D005123:Eye; D005456:Fluorescent Dyes; D007457:Iodine Radioisotopes; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D018715:Microscopy, Video; D008959:Models, Neurological; D009411:Nerve Endings / Q000502:physiology*; D020932:Nerve Growth Factor / Q000378:metabolism*; D009414:Nerve Growth Factors / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D020933:Neurotrophin 3 / Q000378:metabolism; D015388:Organelles / Q000502:physiology*; D017475:Receptors, Nerve Growth Factor / Q000502:physiology; D012235:Rhodamines; D017783:Superior Cervical Ganglion / Q000502:physiology*; D012668:Trigeminal Ganglion / Q000502:physiology*
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D005456:Fluorescent Dyes; D007457:Iodine Radioisotopes; D009414:Nerve Growth Factors; D020933:Neurotrophin 3; D017475:Receptors, Nerve Growth Factor; D012235:Rhodamines; D020932:Nerve Growth Factor; C073922:neurotrophin 4