title: Changes in detrusor and urinary growth factors according to detrusor function after partial bladder outlet obstruction in the rat.
abstract: OBJECTIVE
  To investigate the changes in detrusor and urinary growth factors according to detrusor function after partial bladder outlet obstruction in the rat. Growth factors have been reported to play a role in the remodeling of the bladder after obstruction.
  
  
  METHODS
  Female rats (n = 40) were used and divided into three groups: sham-operated control, 2-week mild obstruction, and 2-week severe obstruction. The voided urine was collected and the detrusor contractility to electrical stimulation recorded. mRNA expression and the urinary concentration of transforming growth factor beta-1 (TGF-beta1) and basic fibroblast growth factor (bFGF) were analyzed.
  
  
  RESULTS
  The contractile response to electrical stimulation was increased in the mild obstruction group (P <0.05) but decreased in the severe obstruction group (P <0.05). The detrusor mRNA expression of TGF-beta1 in the mild obstruction group was not different from that in the control group. However, in the severe obstruction group, the mRNA expression of TGF-beta1 increased. The detrusor mRNA expression of bFGF was gradually increased in the mild and severe obstruction groups. The urinary concentration of TGF-beta1 in the mild obstruction group was not significantly different from that in the control group. However, in the severe obstruction group, the concentration of TGF-beta1 was significantly increased (P <0.05). The concentration of urinary bFGF was too small to compare the results in each group.
  
  
  CONCLUSIONS
  Growth factors such as TGF-beta1 and bFGF change in mRNA expression according to bladder function, and urinary TGF-beta1 reflects the change of detrusor mRNA. Thus, changes in TGF-beta1 may be a marker to evaluate detrusor function.
authors.affiliation: Department of Urology, Catholic University of Korea College of Medicine, Seoul, South Korea.
authors.forename: J
authors.initials: J
authors.lastname: Chul Kim
authors.forename: S
authors.initials: S
authors.lastname: Il Seo
authors.forename: Y
authors.initials: Y
authors.lastname: Hyun Park
authors.forename: T A
authors.initials: TA
authors.lastname: Kon Hwang
mesh_terms: D000818:Animals; D004558:Electric Stimulation; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000652:urine*; D009119:Muscle Contraction / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D016212:Transforming Growth Factor beta / Q000652:urine*; D053773:Transforming Growth Factor beta1; D001748:Urinary Bladder Neck Obstruction / Q000503:physiopathology* / Q000652:urine
chemical_list: D012333:RNA, Messenger; C508962:Tgfb1 protein, rat; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D016222:Fibroblast Growth Factor 2