title: Increased extracellular glutamate in rat prefrontal cortex: movement artifact contribution.
abstract: Extracellular glutamate levels were measured by microdialysis in the prefrontal cortex (PFC) of anaesthetised rats in response to a short, experimenter-provoked mechanical movement of the animal head. Movement caused significant, nerve impulse-independent elevations of glutamate levels (maximum increase, 300+/-30% of baseline). This study reveals a possible artifact in the measurement of extracellular glutamate concentrations by microdialysis and suggests that, in awake animals, treatments associated with stimulation of motor activity can cause non-specific efflux of glutamate in the PFC.
authors.affiliation: Department of Neuroscience and C.N.R. Centre for Neuropharmacology, University of Cagliari, via Porcell 4, 09124 Cagliari, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Carboni
authors.forename: Z L
authors.initials: ZL
authors.lastname: Rossetti
mesh_terms: D000758:Anesthesia; D000818:Animals; D001143:Arousal / Q000502:physiology; D016477:Artifacts; D005110:Extracellular Space / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism*; D008297:Male; D017551:Microdialysis / Q000592:standards*; D009043:Motor Activity / Q000502:physiology; D009068:Movement / Q000502:physiology*; D017397:Prefrontal Cortex / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D018698:Glutamic Acid