Development and fate of crescentic and granulomatous lesions in rat Masugi nephritis.
It has been observed that with Masugi nephritis in Wistar rats the initiation of endocapillary proliferative changes with macrophage accumulation is usually followed by glomerular sclerosis without extracapillary extension. In the present study, the provocation of an extracapillary lesion was attempted using accelerated Masugi nephritis in Wistar-Kyoto rats. In order to accelerate the accumulation of monocyte/macrophages, the administration of methylcellulose was added in an additional group. The development and fate of extracapillary lesions were analyzed histopathologically and immunohistochemically. As a result, the formation of extracapillary proliferation of granulomatous lesions could be initiated in this model. Granulomatous lesions were composed of CD4+ T cells and CD8+ T cells and monocyte/macrophages including multinucleated giant cells. These inflammatory cells had seemingly escaped from the capillary lumen through the injured glomerular basement membrane and formed cellular and granulomatous crescents. In addition, tenascin was strongly expressed in cellular crescents and was a unique extracellular matrix at this cellular stage. The cellular crescents then progressed to sclerosis with the formation of increased collagenous extracellular matrix. These results suggest that a delayed-type hypersensitivity plays a role in granulomatous crescent formation, even though the initial glomerular injury was evoked by a humoral antibody.
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Department of Pathology, Shinshu University School of Medicine, Matsumoto, Japan. tetsuro@po.cnet.ne.jp
D000818:Animals; D019867:Anti-Glomerular Basement Membrane Disease / Q000276:immunology / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D001485:Basement Membrane / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000473:pathology; D018414:CD8-Positive T-Lymphocytes / Q000473:pathology; D004195:Disease Models, Animal; D005620:Freund's Adjuvant; D005923:Glomerulosclerosis, Focal Segmental / Q000276:immunology / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D006099:Granuloma / Q000473:pathology*; D007150:Immunohistochemistry; D007678:Kidney Glomerulus / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D008264:Macrophages / Q000473:pathology; D008297:Male; D008747:Methylcellulose / Q000494:pharmacology; D010710:Phosphates / Q000378:metabolism; D011507:Proteinuria / Q000503:physiopathology; D011817:Rabbits; D051381:Rats; D011921:Rats, Inbred WKY
D010710:Phosphates; C090967:tenacin; D008747:Methylcellulose; D005620:Freund's Adjuvant