[Effect of T-lymphocytes on hematopoietic progenitors in patients with paroxysmal nocturnal hemoglobinuria].
OBJECTIVE
To study the effect of T-lymphocytes on hematopoietic progenitors in patients with paroxysmal nocturnal hemoglobinuria (PNH).


METHODS
Phytohemagglutinin-stimulating T-lymphocytes conditioned medium(PHA-TCM) prepared from PNH(PHA-TCM-PNH) and normal blood(PHA-TCM-N) was added to the cultures of PNH or normal CFU-GM and BFU-E.


RESULTS
In cultures containing PHA-TCM-PNH prepared from newly diagnosed PNH patients, the yields of CFU-GM and BFU-E from either PNH or normal bone marrow were reduced more than they did in cultures containing PHA-TCM-N; but in cultures containing PHA-TCM-PNH prepared from PNH patients in remission, they were normal.


CONCLUSIONS
T-lymphocytes from newly diagnosed PNH patients exerts an inhibitory effect on the in vitro growth of CFU-GM and BFU-E.
Gao
Q
Q
Department of Hematology, First Affiliated Hospital, Hubei Medical University, Wuhan 430060.
Xia
H
H
Chen
Y
Y
D000293:Adolescent; D000328:Adult; D018957:CD59 Antigens / Q000378:metabolism; D002478:Cells, Cultured; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D006457:Hemoglobinuria, Paroxysmal / Q000473:pathology*; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000378:metabolism*
D018957:CD59 Antigens; D007376:Interleukin-2; D016178:Granulocyte-Macrophage Colony-Stimulating Factor