title: Comparative feline genomics: a BAC/PAC contig map of the major histocompatibility complex class II region.
abstract: The genome organization of the human major histocompatibility complex (MHC) will be best understood in a comparative evolutionary context. We describe here the construction of a physical map for the feline MHC. A large-insert domestic cat genomic DNA library was developed using a P1 artificial chromosome (PAC) with a genomic representation of 2.5x and an average insert size of 80 kb. A sequence-ready 660-kb bacterial artificial chromosome/PAC contig map of the domestic cat MHC class II region was constructed with a gene order similar to, but distinct from, that of human and mice: DPB/DPA, Ring3, DMB, TAP1, DOB, DRB2, DRA3, DRB1, DRA2, and DRA1. Fluorescence in situ hybridization analyses of selected class II PAC clones confirmed that the class II region lies in the pericentromeric region of cat chromosome B2. However, apparently unlike the human and mouse MHCs, the domestic cat DRA and DRB genes have undergone multiple duplications and the DQ region has been deleted.
authors.affiliation: Intramural Research Support Program, SAIC-Frederick, Frederick, Maryland 21702-1201, USA. beck@mail.ncifcrf.gov
authors.forename: T W
authors.initials: TW
authors.lastname: Beck
authors.forename: J
authors.initials: J
authors.lastname: Menninger
authors.forename: G
authors.initials: G
authors.lastname: Voigt
authors.forename: K
authors.initials: K
authors.lastname: Newmann
authors.forename: Y
authors.initials: Y
authors.lastname: Nishigaki
authors.forename: W G
authors.initials: WG
authors.lastname: Nash
authors.forename: R M
authors.initials: RM
authors.lastname: Stephens
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: P J
authors.initials: PJ
authors.lastname: de Jong
authors.forename: S J
authors.initials: SJ
authors.lastname: O'Brien
authors.forename: N
authors.initials: N
authors.lastname: Yuhki
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D002415:Cats / Q000235:genetics*; D002874:Chromosome Mapping; D002875:Chromosomes; D022202:Chromosomes, Artificial, Bacterial; D020451:Contig Mapping; D004262:DNA Restriction Enzymes / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D015723:Gene Library; D005802:Genes, MHC Class II; D016678:Genome; D006682:HLA-DP Antigens / Q000235:genetics; D006684:HLA-DR Antigens / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D051379:Mice; D008957:Models, Genetic; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000378:metabolism
chemical_list: D006682:HLA-DP Antigens; D006684:HLA-DR Antigens; D015345:Oligonucleotide Probes; D004262:DNA Restriction Enzymes