Development of a technology for automation and miniaturization of protein crystallization.
The usage of standard 96 well microplates for the screening of crystallization conditions of recombinant proteins offers several advantages when compared to commonly used crystallization plate formats. The adoption of robotic technology for plate and glass slide preparation within a "hanging drop" vapour diffusion crystallization experiment enables to work with an increased throughput at reduced costs. In addition to commercial pipetting devices with a 96-channel aspirator/dispenser, solenoid ink-jet technology was applied to form 250 nl droplets with a diameter of approximately 1 mm. This allows miniaturization of crystallization screening set-ups with an estimated ten-fold cost reduction when compared to commonly used 24 well plates.
Mueller
U
U
Freie Universität Berlin, Institute for Crystallography, Takustr. 6, D-14195, Berlin, Germany.
Nyarsik
L
L
Horn
M
M
Rauth
H
H
Przewieslik
T
T
Saenger
W
W
Lehrach
H
H
Eickhoff
H
H
D003208:Concanavalin A / Q000737:chemistry; D003460:Crystallization; D003461:Crystallography / Q000295:instrumentation* / Q000379:methods*; D007202:Indicators and Reagents; D008904:Miniaturization / Q000295:instrumentation* / Q000379:methods*; D009113:Muramidase / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry*; D012996:Solutions
D007202:Indicators and Reagents; D011994:Recombinant Proteins; D012996:Solutions; D003208:Concanavalin A; D009113:Muramidase