Endogenous production of IL-8 by human colorectal cancer cells and its regulation by cytokines.
This study investigated the endogenous production of the pro-inflammatory and angiogenic cytokine IL-8 by human colorectal cancer cells. We describe substantial (>0.5 ng/ml) constitutive production of IL-8 by certain human colorectal cancer cell lines without the requirement for exogenous stimulation. Tumour cell-derived IL-8 production was upregulated by the addition of the pro-inflammatory type 1 cytokines TNF alpha, IL-1 beta and IFN gamma. Addition of the regulatory type 2 cytokines IL-4 and IL-10 produced paradoxical stimulation of IL-8 production in certain cell lines and downregulation of IL-8 production in others. These results suggest that tumour-derived cytokine production may have an important role in patients with colorectal cancer. Furthermore, they demonstrate the complexity of tumour cell cytokine production and highlight the difficulties in developing effective therapeutic biological response strategies.
Wigmore
S J
SJ
University Department of Surgery, Royal Infirmary of Edinburgh, Edinburgh, EH3 9YW, UK.
Maingay
J P
JP
Fearon
K C
KC
Ross
J A
JA
D015179:Colorectal Neoplasms / Q000378:metabolism*; D016207:Cytokines / Q000494:pharmacology*; D015536:Down-Regulation; D006801:Humans; D007167:Immunotherapy; D016209:Interleukin-8 / Q000096:biosynthesis*; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000187:drug effects; D015854:Up-Regulation
D016207:Cytokines; D016209:Interleukin-8