The expression of substance P and its neurokinin-1 receptor mRNA in the bovine corpus luteum of early developmental stage.
Related to all leukocytes, 90% of eosinophils are recruited into the bovine corpus luteum of early developmental stage. We here describe a simultaneous appearance of substance P (SP)-positive fibre-like structures and the neurokinin-1 (NK-1) receptor mRNA for SP. Substance P was depicted by using indirect immunohistology and immunofluorescence localization. The dot blot analysis confirmed the presence of SP at the protein level. Using nested reverse transcribed-polymerase chain reaction (RT-PCR) analysis, a 358 bp long partial bovine receptor mRNA for SP (NK-1) was sequenced in the spinal cord. The mRNA for SP and for the NK-1 receptor were then detected in the corpus luteum of early developmental stage with RT-PCR and nested RT-PCR. We conclude: The production of SP and the expression of NK-1 receptor mRNA may be involved in the selective recruitment of eosinophils into the bovine corpus luteum of early developmental stage.
Reibiger
I
I
Department of Anatomy, University of Leipzig, Liebigstrasse 13, D-04103 Leipzig, Germany reibiger@medizin.uni-leipzig.de
Aust
G
G
Tscheudschilsuren
G
G
Beyer
R
R
Gebhardt
C
C
Spanel-Borowski
K
K
D000818:Animals; D002417:Cattle; D002465:Cell Movement / Q000502:physiology; D003338:Corpus Luteum / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D004804:Eosinophils / Q000166:cytology / Q000378:metabolism; D005260:Female; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D018040:Receptors, Neurokinin-1 / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013373:Substance P / Q000235:genetics* / Q000378:metabolism
D012333:RNA, Messenger; D018040:Receptors, Neurokinin-1; D013373:Substance P