Analysis of gastrin receptor gene expression in proliferating cells in the neck zone of gastric fundic glands using laser capture microdissection.
Gastrin stimulates proliferation of progenitor cells in the neck zone of gastric fundic mucosa. However, whether it directly enhances this proliferation through its receptors remains unclear. We investigated the expression of gastrin receptors in neck zone proliferating cells in rat gastric fundic glands using a reverse transcription polymerase chain reaction (RT-PCR) coupled with laser capture microdissection and in situ RT-PCR. Gastrin receptor expression was identified in c-fos-expressing cells located in the neck zone, and results of the RT-PCR analysis argued against contamination by other cells, such as enterochromaffin-like, parietal or D cells. Supporting this finding, gastrin receptor gene expression was identified in the neck zone as well as base glands by in situ RT-PCR. Therefore, it is suggested that proliferating cells in the neck zone are stimulated directly by gastrin via their gastrin receptors.
Kazumori
H
H
Second Department of Internal Medicine, Shimane Medical University, Izumo, Shimane, Japan.
Ishihara
S
S
Kawashima
K
K
Fukuda
R
R
Chiba
T
T
Kinoshita
Y
Y
D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D000818:Animals; D002455:Cell Division; D004210:Dissection / Q000379:methods; D005748:Gastric Fundus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005753:Gastric Mucosa / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005755:Gastrins / Q000097:blood; D005786:Gene Expression Regulation / Q000187:drug effects; D007150:Immunohistochemistry; D064747:Lansoprazole; D007834:Lasers; D008297:Male; D009853:Omeprazole / Q000008:administration & dosage / Q000031:analogs & derivatives*; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011949:Receptors, Cholecystokinin / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013270:Stomach / Q000166:cytology / Q000187:drug effects
D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D005755:Gastrins; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger; D011949:Receptors, Cholecystokinin; D064747:Lansoprazole; D009853:Omeprazole