[A recombinant fowlpox virus expressing the fusion protein of Newcastle disease virus strain F48E8 and its protective efficacy].
The transfer vector pFGF1175-1 was constructed by insertion the fusion protein gene of Newcastle disease virus (NDV) F48E8 strain into the insertion vector pFG1175-1, downstream of P7.5 promotor, and then transfected chicken embryo fibroblast (CEF) cell cultures which had been infected with fowlpox virus (FPV) Chinese vaccine strain 282E4 for 3-4 hours. Recombinant FPV with blue plaques were selected and purified in CEF cell culture laid agar containing X-gal. The recombinant FPV named rFPV-NDF was confirmed expressing NDV fusion protein by indirect immunofluorescence assay, and could protect chickens against virulent NDV challenge, The protective rate was 96.7%.
Wu
Y T
YT
Ministry Key Laboratory of Animal Infectious Diseases, Yangzhou University, Yangzhou 225009.
Peng
D X
DX
Liu
X F
XF
Liu
W Z
WZ
Zhang
R K
RK
D000818:Animals; D002642:Chick Embryo; D005587:Fowlpox virus / Q000235:genetics*; D009522:Newcastle disease virus / Q000276:immunology*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology*; D014760:Viral Fusion Proteins / Q000096:biosynthesis* / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D014760:Viral Fusion Proteins; D014765:Viral Vaccines