title: Chemopreventive agent resveratrol, a natural product derived from grapes, reversibly inhibits progression through S and G2 phases of the cell cycle in U937 cells.
abstract: Resveratrol, a natural product derived from grapes, has been shown to prevent carcinogenesis in murine models. We report here that resveratrol induces antiproliferation and arrests the S phase in human histiocytic lymphoma U937 cells. Resveratrol induces arrest in the S phase at low concentrations (30-60 microM), but high concentrations do not induce S phase accumulation in U937 cells. Removal of resveratrol from the culture medium stimulates U937 cells to reenter the cell cycle synchronously, as judged by the expression patterns of cyclin E, A and by fluorescent activated cell sorting analysis. These data demonstrate that resveratrol causes S phase arrest and reversible cell cycle arrest. Thus, resveratrol provides an important new cell cycle blocker as well as a cancer chemopreventive agent.
authors.affiliation: Department of Immunology, School of Medicine, Keimyung University, 194 Dong San-Dong Jung-Gu, Taegu 700-712, South Korea.
authors.forename: J W
authors.initials: JW
authors.lastname: Park
authors.forename: Y J
authors.initials: YJ
authors.lastname: Choi
authors.forename: M A
authors.initials: MA
authors.lastname: Jang
authors.forename: Y S
authors.initials: YS
authors.lastname: Lee
authors.forename: D Y
authors.initials: DY
authors.lastname: Jun
authors.forename: S I
authors.initials: SI
authors.lastname: Suh
authors.forename: W K
authors.initials: WK
authors.lastname: Baek
authors.forename: M H
authors.initials: MH
authors.lastname: Suh
authors.forename: I N
authors.initials: IN
authors.lastname: Jin
authors.forename: T K
authors.initials: TK
authors.lastname: Kwon
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D015153:Blotting, Western; D002452:Cell Count; D002453:Cell Cycle / Q000187:drug effects; D018797:Cell Cycle Proteins / Q000187:drug effects / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D016195:G2 Phase / Q000187:drug effects*; D006801:Humans; D000077185:Resveratrol; D019685:Rosales / Q000737:chemistry*; D016196:S Phase / Q000187:drug effects*; D013267:Stilbenes / Q000494:pharmacology*; D013997:Time Factors; D020298:U937 Cells
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D018797:Cell Cycle Proteins; D013267:Stilbenes; D000077185:Resveratrol