Improved determination of urinary oxalate with alkylamine glass bound barley oxalate oxidase.
The measurement of oxalate in urine was improved by employing barley oxalate oxidase immobilized on alkylamine glass beads affixed in a glass beaker. The minimum detection limit was 3.6 mg l(-1) urine. The recovery of added oxalate was 88.9+/-9.2%. Within- and between-assay coefficients of variation (CV) were <4.0 and <9.4%, respectively. The urinary oxalate values were obtained by a commercial kit method and the present method showed a good correlation (0.999). The method is free from tedious handling of glass beads and Cl- interference.
Chandran
P
P
Biochemistry Research Laboratory, Department of Bio-Sciences, M.D. University, Rohtak-124001, Haryana, India.
Thakur
M
M
Pundir
C S
CS
D000328:Adult; D000588:Amines / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry; D005898:Glass; D001467:Hordeum / Q000201:enzymology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D008863:Microspheres; D010070:Oxalates / Q000652:urine*; D010088:Oxidoreductases / Q000032:analysis*; D010940:Plant Proteins / Q000737:chemistry; D013696:Temperature; D013997:Time Factors
D000588:Amines; D004800:Enzymes, Immobilized; D010070:Oxalates; D010940:Plant Proteins; D010088:Oxidoreductases; C020598:oxalate oxidase