title: Chronic restraint stress induces severe disruption of the T-cell specific response to tetanus toxin vaccine.
abstract: Chronic stress is known to induce immunological disorders. In the present study we examined the consequences of chronic restraint stress on the immune response to tetanus toxin in mice. We investigated the repartition of subsets of lymphoid cells in blood and spleen, the functional ability of lymphocytes to proliferate and to produce cytokines, and antibody titres against tetanus toxin following stress. We report discordance of the stimulation index of lymphocytes in the restraint group: the proliferating rate severely decreased following stimulation with a relevant antigen, whereas it increased with mitogen. Thus, we report a decrease in cytokine production with relevant antigen (interferon-gamma and interleukin-10), without a T helper type 1 and 2 secretion imbalance. Moreover, we observed an alteration in the humoral response, including a delay in isotype maturation and an immunoglobulin G1/G2a imbalance.
authors.affiliation: Départements de biologie des agents transmissibles et de radiobiologie, CRSSA, 38702 La Tronche, France.
authors.forename: J N
authors.initials: JN
authors.lastname: Tournier
authors.forename: J
authors.initials: J
authors.lastname: Mathieu
authors.forename: Y
authors.initials: Y
authors.lastname: Mailfert
authors.forename: E
authors.initials: E
authors.lastname: Multon
authors.forename: C
authors.initials: C
authors.lastname: Drouet
authors.forename: A
authors.initials: A
authors.lastname: Jouan
authors.forename: E
authors.initials: E
authors.lastname: Drouet
mesh_terms: D000818:Animals; D002455:Cell Division / Q000276:immunology; D002908:Chronic Disease; D003208:Concanavalin A / Q000276:immunology; D003345:Corticosterone / Q000097:blood; D016207:Cytokines / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000276:immunology; D016131:Lymphocyte Subsets / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000276:immunology; D013312:Stress, Physiological / Q000097:blood / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013744:Tetanus Toxin / Q000276:immunology*
chemical_list: D016207:Cytokines; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D013744:Tetanus Toxin; D003208:Concanavalin A; D003345:Corticosterone