Congenic strains developed for alcohol- and drug-related phenotypes.
Quantitative trait loci (QTLs) for many alcohol- and drug-related traits have been mapped using well-accepted mapping techniques. The ultimate goal of gene identification necessitates confirmation of the QTL and reduction of the interval surrounding the QTL; both can be accomplished in congenic strains. These strains carry a chromosomal region introgressed from a donor strain onto the genetic background of a second, recipient strain. Multiple generations of backcrossing reduce the unlinked donor genome to less than 0.1%. Then, phenotypic comparisons between mice congenic for the donor region and controls from the recipient strain allow confirmation of the QTL effect. Animals with recombinations in the donor region can be used to generate interval-specific congenic recombinant lines. Numerous congenic strains are currently being developed in which chromosomal regions carrying QTLs for alcohol- and drug-related traits have been transferred from one strain onto a second strain. The purpose of this review is to summarize the chromosomal regions, donor and recipient strains, and results obtained from these congenics. Most researchers developing such strains are willing to share these resources to facilitate localization of the genetic bases of other phenotypes.
Bennett
B
B
Institute for Behavioral Genetics, CB 447, University of Colorado, Boulder, CO 80309-0447, USA. bennettb@coloradu.edu
D000437:Alcoholism / Q000235:genetics; D000818:Animals; D002874:Chromosome Mapping / Q000379:methods; D002875:Chromosomes / Q000235:genetics; D007178:Inbreeding; D051379:Mice; D020297:Mice, Congenic / Q000235:genetics*; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009043:Motor Activity / Q000235:genetics; D010641:Phenotype; D019655:Quantitative Trait, Heritable; D013375:Substance Withdrawal Syndrome / Q000235:genetics*; D019966:Substance-Related Disorders / Q000235:genetics*