[Secretive activity of tumor necrosis factor alpha (TNF alpha) of marrow stromal cells in acute leukemia and its clinical significance].
OBJECTIVE
To investigate the TNF alpha secreting activity of marrow stromal cells in acute leukemia.


METHODS
TNF alpha in vitro culture supernate of marrow stromal cells from 20 acute leukemia patients and 10 normal controls was detected. TNF alpha activities in patients and control serum and in patients leukemic cell culture supernate were also detected.


RESULTS
TNF alpha activity in leukemic stromal cell supernate was significantly higher than that of the control (P < 0.05). Some AML cell could spontaneously secret TNF alpha. Serum TNF alpha activity of the patient group is also evidently higher than that of the control group (P < 0.001).


CONCLUSIONS
There were qualitative and quantitative defects of marrow stromal cells in acute leukemia patients. Increased secretion of TNF alpha is probably an important factor leading to the abnormal proliferation of acute leukemia cells.
Hou
L
L
Laboratory of Hematology, First Affiliated Hospital, China Medical University, Shenyang 110001.
Zhai
M
M
Lu
X
X
D000293:Adolescent; D000328:Adult; D000368:Aged; D001853:Bone Marrow / Q000378:metabolism; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000378:metabolism*; D017154:Stromal Cells / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D014409:Tumor Necrosis Factor-alpha