In vivo synergy between green tea extract and levofloxacin against enterohemorrhagic Escherichia coli O157 infection.
We studied the synergistic effects of Japanese green tea extract (JGTE) and levofloxacin (LVFX) against enterohemorrhagic Escherichia coli (EHEC) infection in a gnotobiotic mouse model. Mice fed on JGTE conferred a significant degree of protection against an oral challenge with EHEC. Complete elimination of the bacteria from the mice, was however, difficult. The combination of JGTE and LVFX increased the survival rate and reduced damage to target organs. Thus, dietary supplementation with JGTE improved the therapeutic effects of antibiotic treatment.
Isogai
E
E
Dept. of Preventive Dentistry, Health Sciences Univ. of Hokkaido, Ishikari-Tobetsu 1757, Hokkaido 061-0293, Japan. emiko@hoku-igro-u.ac.jp
Isogai
H
H
Hirose
K
K
Hayashi
S
S
Oguma
K
K
D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002392:Catechin / Q000494:pharmacology*; D015169:Colony Count, Microbial; D004357:Drug Synergism; D004797:Enzyme-Linked Immunosorbent Assay; D004927:Escherichia coli Infections / Q000188:drug therapy*; D019453:Escherichia coli O157 / Q000187:drug effects / Q000378:metabolism; D005243:Feces / Q000382:microbiology; D005260:Female; D005856:Germ-Free Life; D064704:Levofloxacin; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D015242:Ofloxacin / Q000494:pharmacology* / Q000627:therapeutic use; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D022621:Shiga Toxin / Q000378:metabolism; D013662:Tea / Q000737:chemistry*
D000890:Anti-Infective Agents; D010936:Plant Extracts; D013662:Tea; D064704:Levofloxacin; D022621:Shiga Toxin; D002392:Catechin; D015242:Ofloxacin