Comparison of polymerase chain reaction, immunohistochemistry and conventional histopathology in the diagnosis of early leprosy in Sichuan Province of China.
46 formalin-fixed, paraffin-embedded skin biopsy specimens, which were clinically suspected or diagnosed as early leprosy, were retrieved from the files of Sichuan, China from 1997 to 1999. All of them were examined by polymerase chain reaction (PCR) using the primers amplifying the 130 base-pair fragment of the gene from the 16S ribosomal RNA of Mycobacterium leprae, hematoxylin and eosin (H&E) staining, modified Fite-Faraco technique for M. leprae and immunostaining with the antiserum against the PGL-1, LAM-B, S-100 protein using ABC method. PCR was positive for 27 (58.7%) of 46 specimens. In 13 (28.3%) among them, only PCR signals were positive for M. leprae and all other test were negative. AFB was positive for 7 (15.2%) of 46, PGL-1 was positive for 17 (36.9%) of 46, LAM-B was positive for 10 (21.7%) of 46. Early epithelioid cells granuloma was detected in 4 (8.7%) patients (TT 3, BT 1), macrophage granuloma was detected in 1 (2.2%) patient (BL), S-100 protein staining showed early nerve granuloma for 4 (8.7%) of 46, peripheral nerve inflammatory infiltration for 11 (23.9%) of 46. Comparison PCR with other method showed statistically significant difference. PCR have an advantage over microscopic examination in detecting M. leprae in biopsy specimens negative for acid-fast bacilli.
Shi
L
L
Sichuan Provincial Institute of Dermatology and Venereology, 12 Sidao Street, Chengdu, P.R. China. shiling98@hotmail.com
Yajima
M
M
Kawatsu
K
K
Matsuoka
M
M
Kashiwabara
Y
Y
Endoh
M
M
D002681:China / Q000453:epidemiology; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D007918:Leprosy / Q000175:diagnosis* / Q000382:microbiology / Q000473:pathology; D009166:Mycobacterium leprae / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012867:Skin / Q000382:microbiology* / Q000473:pathology
D004269:DNA, Bacterial