[Induction of apoptosis of CD34+ cells by serum from two patients with severe aplastic anemia].
OBJECTIVE
To explore the relationship between the hematopoietic inhibitor and apoptosis of CD34+ cells in patients with severe aplastic anemia (SAA).


METHODS
The inhibiting activity to the growth of both BFU-E and CFU-GM was analysed by in vitro methylcellulose culture and CD34+ cell apoptosis was assayed with a kit of programmed   cell death assay and flow cytometry.


RESULTS
Sera from two SAA patients suppressed the growth of normal hematopoietic progenitor cells and induced CD34+ cell apoptosis. Serum immunoglobulin fraction assay showed that the inhibiting activity was in the IgG fraction which bound specifically to CD34+ cells.


CONCLUSIONS
There was an inhibitive IgG fraction, which induced CD34+ cell apoptosis, in the serum of SAA patients and CD34+ cell apoptosis might be a part of the pathogenesis of SAA.
Liu
H
H
Department of Hematology, Affiliated Hospital of Nantong Medical College, Nantong 226001.
Wu
F
F
Jiang
S
S
D000293:Adolescent; D000368:Aged; D000741:Anemia, Aplastic / Q000097:blood* / Q000188:drug therapy / Q000276:immunology; D018952:Antigens, CD34 / Q000378:metabolism*; D017209:Apoptosis; D002455:Cell Division; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D005260:Female; D005938:Glucocorticoids / Q000627:therapeutic use; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007074:Immunoglobulin G / Q000494:pharmacology; D008297:Male; D008775:Methylprednisolone / Q000627:therapeutic use
D018952:Antigens, CD34; D017077:Culture Media, Conditioned; D005938:Glucocorticoids; D007074:Immunoglobulin G; D008775:Methylprednisolone