Interference between host resistance to Listeria monocytogenes infection and ovalbumin-induced allergic responses in mice.
Listeria monocytogenes promotes the induction of the T-helper 1 (Th1) cell response, while ovalbumin (OVA) induces a Th2 cell response and allergic reactions, such as airway hyperreactivity and immunoglobulin E (IgE) production. When mice were immunized with OVA on day 7 after L. monocytogenes infection, eosinophilia in bronchoalveolar lavage and the production of total IgE, OVA-specific IgE, interleukin-4 (IL-4), and IL-5 in the circulation were markedly suppressed. Cytokine responses, including IL-4, IL-5, IL-10, IL-13, and gamma interferon, to OVA were decreased in the spleen cell cultures obtained from OVA-immunized mice that had been infected with L. monocytogenes. Conversely, when OVA-immunized mice were infected with L. monocytogenes, conversion from the nonlethal infection to the lethal infection occurred. Host resistance to L. monocytogenes infection in OVA-immunized mice was enhanced by the administration of anti-IL-10 monoclonal antibody. The present study indicates that striking interference is observed between Th1-inducing L. monocytogenes infection and Th2-driven OVA-induced airway hyperreactivity.
Mizuki
D
D
Department of Bacteriology, School of Medicine, Hirosaki University, Hirosaki, Japan.
Miura
T
T
Sasaki
S
S
Mizuki
M
M
Madarame
H
H
Nakane
A
A
D000818:Animals; D000918:Antibody Specificity; D016207:Cytokines / Q000032:analysis; D004802:Eosinophilia; D005260:Female; D007113:Immunity, Innate; D007073:Immunoglobulin E; D008088:Listeriosis / Q000150:complications / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000276:immunology*; D012130:Respiratory Hypersensitivity / Q000150:complications / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
D016207:Cytokines; D007073:Immunoglobulin E; D010047:Ovalbumin