New pathway of amine oxidation respiratory chain of Paracoccus denitrificans IFO 12442.
The physiological electron acceptor of quinohemoprotein amine dehydrogenase (QH-AmDH) from Paracoccus denitrificans IFO 12442 was identified by biochemical and electrochemical methods. Of three types of heme c-containing proteins purified together with QH-AmDH from the periplasm of n-butylamine-grown cells, only constitutive cytochrome c-550 was reduced by the addition of QH-AmDH and n-butylamine. Reconstitution of the respiratory chain revealed that cytochrome c-550 mediates the electron transfer from QH-AmDH to the terminal oxidase. This is a new pathway of the amine oxidation respiratory chain of P. denitrificans.
Takagi
K
K
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
Yamamoto
K
K
Kano
K
K
Ikeda
T
T
D000588:Amines / Q000378:metabolism*; D001426:Bacterial Proteins / Q000032:analysis; D002082:Butylamines / Q000378:metabolism; D003574:Cytochrome c Group / Q000378:metabolism; D004563:Electrochemistry; D004579:Electron Transport; D006420:Hemeproteins / Q000302:isolation & purification / Q000378:metabolism*; D008744:Methylamines / Q000378:metabolism; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000302:isolation & purification / Q000378:metabolism*; D010231:Paracoccus denitrificans / Q000201:enzymology*
D000588:Amines; D001426:Bacterial Proteins; D002082:Butylamines; D003574:Cytochrome c Group; D006420:Hemeproteins; D008744:Methylamines; C017079:mauC protein, Methylobacterium extorquens; C031732:cytochrome C-550; C027451:methylamine; D000587:Oxidoreductases Acting on CH-NH Group Donors; C119497:quinohemoprotein amine dehydrogenase; C032462:n-butylamine