Mass spectrometric identification of a candidate biomarker peptide from the in vitro interaction of epichlorohydrin with red blood cells.
The reaction products of epichlorohydrin with human alpha- and beta- globins, obtained through in vitro incubation of these compounds and red blood cells, were determined by using reversed-phase high-performance liquid chromatography (RP-HPLC), electrospray ionization mass spectrometry and matrix-assisted laser desorption/ionization tandem mass spectrometry. The alpha-globin was much more reactive than the beta-globin. At low incubation ratios, approximating the order of magnitude of epichlorohydrin concentration as found in workplaces, the only modified peptide still detectable was the 62-90 belonging to the alpha-chain and carrying an incremental mass of 92 u on either His72 or His89. Given that the two peptides co-eluted in a single chromatographic peak during RP-HPLC separation, they could be chosen as suitable biomarkers for quantification in the setting up of a new methodology for the biological monitoring of persons occupationally exposed, replacing currently known procedures.
Miraglia
N
N
Centro Internazionale di Servizi di Spettrometria di Massa, Istituto di Scienze dell'Alimentazione del CNR, Via Roma 52, I-83100 Avellino Italy.
Pocsfalvi
G
G
Ferranti
P
P
Basile
A
A
Sannolo
N
N
Acampora
A
A
Soleo
L
L
Palmieri
F
F
Caira
S
S
De Giulio
B
B
Malorni
A
A
D000596:Amino Acids / Q000032:analysis; D015415:Biomarkers / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D004811:Epichlorohydrin / Q000097:blood*; D004912:Erythrocytes / Q000737:chemistry*; D005914:Globins / Q000378:metabolism; D006801:Humans; D013058:Mass Spectrometry; D016273:Occupational Exposure; D010446:Peptide Fragments / Q000097:blood; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014357:Trypsin / Q000378:metabolism
D000596:Amino Acids; D015415:Biomarkers; D010446:Peptide Fragments; D004811:Epichlorohydrin; D005914:Globins; D014357:Trypsin