Molecular characterization of rabbit renal epithelial calcium channel.
PCR and antisense oligodeoxy-nucleotide (ODN) blocking were used to identify a calcium (Ca) channel in rabbit proximal tubule (PT) cells. The subcloned Ca channel is identical to the rabbit cardiac Ca channel (alpha(1)) except a 33 base deletion at the fourth S3-S4 linker in PT cells. Anti-sense ODN treatment (18 h) inhibited 73 and 44% of Ca influxes induced by hypoosmotic stress (220 Osm) and by 1-oleoyl-2-acetyl-sn-glycerol (5 microM), respectively. The results indicate that the subcloned channel is a spliced variant of the cardiac Ca channel and that it plays a critical role in regulation of Ca signaling in these cells.
Zhang
M I
MI
Department of Integrative Biology, Physiology, and Pharmacology, Houston Medical School, University of Texas, 6431 Fannin, Houston, Texas, 77030, USA. min.zhang@uth.tmc.edu
O'Neil
R G
RG
D017398:Alternative Splicing / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D030161:Avian Proteins; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D020746:Calcium Channels, L-Type / Q000235:genetics / Q000378:metabolism; D020013:Calcium Signaling / Q000187:drug effects; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004075:Diglycerides / Q000494:pharmacology; D004848:Epithelium / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D015640:Ion Channel Gating / Q000187:drug effects; D007687:Kidney Tubules, Proximal / Q000737:chemistry / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008565:Membrane Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000235:genetics / Q000494:pharmacology; D009997:Osmotic Pressure; D011487:Protein Conformation; D020033:Protein Isoforms / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017384:Sequence Deletion / Q000235:genetics; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D030161:Avian Proteins; C051235:CCC protein, chicken; D015220:Calcium Channels; D020746:Calcium Channels, L-Type; D004075:Diglycerides; D008565:Membrane Proteins; D016376:Oligonucleotides, Antisense; D020033:Protein Isoforms; D012333:RNA, Messenger; C038237:1-oleoyl-2-acetylglycerol; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium