title: Regulation of Differentiation, Proliferation and Drug-Induced Apoptosis in HT58 Lymphoma Cells.
abstract: Recently, it has been suggested, that differentiated cells are more resistant to the apoptotic effect of DNA damaging agents possibly due to the decreased activity of "damage detecting/apoptosis triggering" mechanism. Previously, we have shown, that PMA pretreatment reduced etoposide-(ETO) but enhanced staurosporine- (STA) -induced apoptosis in HT58 cells. Data presented here show that the HT58 human, "mature" B-lymphoma cells exposed to PMA secrete more IgM into the supernatant indicating commitment of cells to perform differentiated function. The sensitivity of HT58 cells to ETO- or STA-induced apoptosis is influenced diversely with PMA pre- or posttreatment. Interestingly, the DNA damage (gamma radiation, bleomycin, ETO) or okadaic acic (30 nM) reduced the [PMA+STA] induced apoptosis.
authors.affiliation: Semmelweis University of Medicine, 1st Institute of Pathology and Experimental Cancer Research, Budapest, Hungary.
authors.forename: Rudolf
authors.initials: R
authors.lastname: Mihalik
authors.forename: Ferenc
authors.initials: F
authors.lastname: Uher
authors.forename: István
authors.lastname: Peták
authors.forename: Anna
authors.initials: A
authors.lastname: Sebestyén
authors.forename: Lászl&oacute
authors.initials: L
authors.lastname: Kopper