Globular tail of myosin-V is bound to vamp/synaptobrevin.
VAMP/synaptobrevin is one of a number of v-SNAREs involved in vesicular fusion events in neurons. In a previous report, VAMP was shown to form a complex with synaptophysin and myosin V, a motor protein based on the F-actin, and that myosin V was then released from the complex in a Ca(2+)-dependent manner. Here, we found that VAMP alone is bound to myosin V in a Ca(2+)-independent manner, and determined that the globular tail domain of myosin V is its binding site. The syntaxin-VAMP-myosin V formed in the presence of Ca(2+)/calmodulin (CaM). In the absence of CaM, only syntaxin-VAMP, or VAMP-myosin V complex was formed. Our results suggest that VAMP acts as a myosin V receptor on the vesicles and regulates formation of the complex.
Ohyama
A
A
Department of Anesthesiology and Reanimatology, Gunma University School of Medicine, Maebashi, Gunma, 371-8511, Japan.
Komiya
Y
Y
Igarashi
M
M
D000199:Actins / Q000737:chemistry; D000818:Animals; D001665:Binding Sites; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002147:Calmodulin / Q000737:chemistry; D002148:Calmodulin-Binding Proteins / Q000737:chemistry*; D018076:DNA, Complementary / Q000378:metabolism; D005089:Exocytosis; D005982:Glutathione Transferase / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D024701:Myosin Type V; D009419:Nerve Tissue Proteins / Q000737:chemistry*; D011485:Protein Binding; D014176:Protein Biosynthesis; D017434:Protein Structure, Tertiary; D050765:Qa-SNARE Proteins; D050683:R-SNARE Proteins; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry; D050600:SNARE Proteins; D016708:Synaptophysin / Q000737:chemistry; D033921:Vesicular Transport Proteins
D000199:Actins; D002147:Calmodulin; D002148:Calmodulin-Binding Proteins; D018076:DNA, Complementary; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D050765:Qa-SNARE Proteins; D050683:R-SNARE Proteins; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D050600:SNARE Proteins; D016708:Synaptophysin; D033921:Vesicular Transport Proteins; C078829:chicken brain myosin-V p190; D005982:Glutathione Transferase; D024701:Myosin Type V; D002118:Calcium