Regulation of glucose transport in differentiating HD3 cells.
The chicken erythroblast cell line, HD3, has high glucose transport activity which is lost upon differentiation to the red cell phenotype. HD3 cells, when incubated under conditions where maturation occurs, show substantial loss of GLUT1 and GLUT3 mRNAs. To assess whether cAMP or cellular protein phosphorylation affected GLUT mRNA and protein, the HD3 cells were incubated in the presence of different phosphatase inhibitors. Treatment of HD3 cells with the phosphatase inhibitors okadaic acid, vanadate or with 3-isobutyl-1-methyl-xanthine induced glucose transport and GLUT mRNAs. This suggests that phosphorylation events enhance glucose transport and that their reduction may be involved in the decrease in glucose transport that occurs upon HD3 cells differentiation.
Grdisa
M
M
Division of Molecular Medicine, Rudjer Boskovic Institute, Bijenicka 54, 10000 Zagreb, Croatia. gridisa@rudjer.irb.hr
White
M K
MK
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002454:Cell Differentiation; D002645:Chickens; D000242:Cyclic AMP / Q000494:pharmacology; D012321:DNA-Directed RNA Polymerases; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004900:Erythroblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D051272:Glucose Transporter Type 1; D051274:Glucose Transporter Type 3; D009004:Monosaccharide Transport Proteins / Q000096:biosynthesis / Q000235:genetics; D009419:Nerve Tissue Proteins; D019319:Okadaic Acid / Q000494:pharmacology; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors; D012323:RNA Processing, Post-Transcriptional; D014638:Vanadates / Q000494:pharmacology; D014764:Viral Proteins
D004791:Enzyme Inhibitors; D051272:Glucose Transporter Type 1; D051274:Glucose Transporter Type 3; D009004:Monosaccharide Transport Proteins; D009419:Nerve Tissue Proteins; C090878:RiSVs1gp1 protein, Rice stripe virus; D014764:Viral Proteins; D019319:Okadaic Acid; D014638:Vanadates; D000242:Cyclic AMP; D012321:DNA-Directed RNA Polymerases; D010749:Phosphoprotein Phosphatases; D005947:Glucose