Directed immobilization of recombinant staphylococci on cotton fibers by functional display of a fungal cellulose-binding domain.
The immobilization of recombinant staphylococci onto cellulose fibers through surface display of a fungal cellulose-binding domain (CBD) was investigated. Chimeric proteins containing the CBD from Trichoderma reesei cellulase Cel6A were found to be correctly targeted to the cell wall of Staphylococcus carnosus cells, since full-length proteins could be extracted and affinity-purified. Furthermore, surface accessibility of the CBD was verified using a monoclonal antibody and functionality in terms of cellulose-binding was demonstrated in two different assays in which recombinant staphylococci were found to efficiently bind to cotton fibers. The implications of this strategy of directed immobilization for the generation of whole-cell microbial tools for different applications will be discussed.
Lehtiö
J
J
Department of Biotechnology, Royal Institute of Technology (KTH), SE-100 44 Stockholm, Sweden.
Wernérus
H
H
Samuelson
P
P
Teeri
T T
TT
Ståhl
S
S
D002473:Cell Wall / Q000378:metabolism; D018914:Cells, Immobilized; D002480:Cellulase / Q000737:chemistry / Q000235:genetics; D002482:Cellulose / Q000378:metabolism*; D043366:Cellulose 1,4-beta-Cellobiosidase; D005822:Genetic Vectors; D003368:Gossypium; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D013210:Staphylococcus / Q000235:genetics / Q000378:metabolism*; D014242:Trichoderma / Q000235:genetics
D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; D002482:Cellulose; D002480:Cellulase; D043366:Cellulose 1,4-beta-Cellobiosidase