title: Characterization of anthocyanin p-coumaroyltransferase in flowers of Iris ensata.
abstract: Malvidin and petunidin 3-(p-coumaroyl)rhamnosylglucoside-5-glucosides as well as nonacylated 3-rhamnosylglucoside-5-glucoside of these anthocyanidins were detected as major anthocyanins in cyanic flowers of Iris ensata. Enzyme extracts from flower buds of this plant catalyzed the transfer of the p-coumaroyl moiety from p-coumaroyl-CoA to both the anthocyanidin 3-rhamnosylglucoside and 3-rhamnosylglucoside-5-glucoside to form the anthocyanidin 3-(p-coumaroyl) rhamnosylglucoside and 3-(p-coumaroyl) rhamnosylglucoside-5-glucoside, at a ratio of ca. 1 to 4, respectively. The activities of this enzyme were also examined for various cyanic and acyanic cultivars, in addition to the characterization of the p-coumaroyltransferase. The sequence of acylation and 5-glucosylation in the anthocyanin biosynthesis of this plant is discussed.
authors.affiliation: Division of Biotechnology and Biochemistry, Faculty of Agriculture, Miyazaki University, 889-2192, Miyazaki, Japan
authors.forename: T
authors.initials: T
authors.lastname: Yabuya
authors.forename: M -a.
authors.initials: M
authors.lastname: Yamaguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Fukui
authors.forename: K
authors.initials: K
authors.lastname: Katoh
authors.forename: T
authors.initials: T
authors.lastname: Imayama
authors.forename: I
authors.lastname: Ino