title: Eggplant lipoxygenase (Solanum melongena): product characterization and effect of physicochemical properties of linoleic acid on the enzymatic activity.
abstract: Lipoxygenase (LOX) from eggplant (Solanum melongena L. cv. Belleza negra) was partially purified, and the products and kinetics of the enzyme were studied. Linoleic acid (LA) was the best substrate for this enzyme. Product analysis by HPLC and GC/MS revealed that, at its pH optimum (pH 7.0), the enzyme converted LA almost totally into the 9-hydroperoxy isomer, whereas the 13-hydroperoxy isomer was only a minor product. At this pH, the enzyme had K(m) and V(max) values for LA of 1.4 microM and 2.2 micromol min(-1) (mg of protein)(-1), respectively, when the monomeric form of LA was used as substrate. The dependence of eggplant LOX activity on the physicochemical properties of LA was also studied. Experiments revealed that LA aggregates were used more efficiently than monomeric LA as substrate. The apparent substrate cooperativity observed may be due to the different activities exhibited toward monomers and aggregates. This result can be interpreted as a substrate-aggregation dependent activity.
authors.affiliation: Departamento de Bioquímica y Biología Molecular-A, Facultad de Biología, Campus de Espinardo, Universidad de Murcia, E-30071 Murcia, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: López-Nicolás
authors.forename: M
authors.initials: M
authors.lastname: Pérez-Gilabert
authors.forename: F
authors.initials: F
authors.lastname: García-Carmona
mesh_terms: D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D019787:Linoleic Acid / Q000737:chemistry* / Q000494:pharmacology*; D008084:Lipoxygenase / Q000378:metabolism*; D013379:Substrate Specificity; D014675:Vegetables / Q000201:enzymology*
chemical_list: D019787:Linoleic Acid; D008084:Lipoxygenase