Green tea polyphenols as potent enhancers of glucocorticoid-induced mouse mammary tumor virus gene expression.
The effect of natural and synthetic galloyl esters on glucocorticoid-induced gene expression was evaluated by using rat fibroblast 3Y1 cells stably transfected with a luciferase reporter gene under the transcriptional regulation of the mouse mammary tumor virus promoter. The glucocorticoid-induced gene transcription was strongly suppressed by synthetic alkyl esters; n-dodecyl gallate showed the most potent inhibition (66% inhibition at 10 microM), which was far more potent than that of crude tannic acid. n-Octyl and n-cetyl gallate also showed good inhibition, while gallic acid itself was not so active, suggesting that the presence of hydrophobic side chain is important for the suppressive effect. On the other hand, surprisingly, green tea gallocatechins, (-)-epigallocatechin-3-O-gallate and theasinensin A, potently enhanced the promoter activity (182 and 247% activity at 1 microM, respectively). The regulation of the level of the glucocorticoid-induced gene expression by the antioxidative gallates is of great interest from a therapeutic point of view.
Abe
I
I
School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Shizuoka, 422-8526, Japan. abei@ys7.u-shizuoka-ken.ac.jp
Umehara
K
K
Morita
R
R
Nemoto
K
K
Degawa
M
M
Noguchi
H
H
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002460:Cell Line; D003907:Dexamethasone / Q000494:pharmacology; D004347:Drug Interactions; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004952:Esters / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D005419:Flavonoids; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D017930:Genes, Reporter; D005938:Glucocorticoids / Q000378:metabolism*; D047348:Hydrolyzable Tannins / Q000494:pharmacology; D008156:Luciferases / Q000378:metabolism; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D008956:Models, Chemical; D010636:Phenols / Q000737:chemistry*; D010957:Plasmids / Q000378:metabolism; D011108:Polymers / Q000737:chemistry*; D011401:Promoter Regions, Genetic; D051381:Rats; D013662:Tea / Q000737:chemistry*; D014158:Transcription, Genetic; D014162:Transfection
D000975:Antioxidants; D004791:Enzyme Inhibitors; D004952:Esters; D005419:Flavonoids; D005938:Glucocorticoids; D047348:Hydrolyzable Tannins; D010636:Phenols; D011108:Polymers; D013662:Tea; C008259:lauryl gallate; D005707:Gallic Acid; D003907:Dexamethasone; D002392:Catechin; C045651:epigallocatechin gallate; D008156:Luciferases