title: Effect of six compounds isolated from rhizome of Anemone raddeana on the superoxide generation in human neutrophil.
abstract: The effect of six compounds isolated from rhizome of Anemone raddeana on the superoxide generation in human neutrophils was investigated. The six compounds examined were 3-acetyloleanolic acid (AOA), oleanolic acid (OA), eleutheroside K (EK), oleanolic acid-3-O-alpha-L-rhamnopyranosyl-(1 --> 2)-[beta-D-glucopyranosyl-(1 --> 4)]-alpha-L-arabinopyranoside (Rd10), raddeanoside 12 (Rd12) and raddeanoside 13 (Rd13). AOA, OA, Rd12 and Rd13 suppressed the superoxide generation induced by N-formyl-methionyl-leucyl-phenylalanine (fMLP) in a concentration-dependent manner. EK and Rd10 significantly enhanced the fMLP-induced superoxide generation in a specific narrow range of low concentration (0.5-0.75 microM), while these compounds more efficiently suppressed the superoxide generation than the other four compounds in other concentrations. In the case of superoxide generation induced by phorbol 12-myristate 13-acetate (PMA), Rd12, OA, EK and Rd10 dose-dependently suppressed the superoxide generation but AOA and Rd13 gave no effect. Arachidonic acid-induced superoxide generation was suppressed by EK, Rd10, Rd12 and Rd13, but was weakly enhanced by AOA and OA. Rd12 dose-dependently inhibited fMLP-induced tyrosyl phosphorylation of 123.0, 79.4, 60.3, 56.2 and 50.1 kDa proteins in human neutrophil. On the other hand, RD10 and EK enhanced the tyrosyl phosphorylation of these proteins in a low concentration range. These phenomena were parallel to the suppression of the fMLP-induced superoxide generations.
authors.affiliation: Department of Pharmacognosy, Shenyang Pharmaceutical University, No. 103, Wenhua Rood, Shenyang 110015, China.
authors.forename: J
authors.initials: J
authors.lastname: Lu
authors.forename: Q
authors.initials: Q
authors.lastname: Sun
authors.forename: K
authors.initials: K
authors.lastname: Sugahara
authors.forename: Y
authors.initials: Y
authors.lastname: Sagara
authors.forename: H
authors.initials: H
authors.lastname: Kodama
mesh_terms: D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D019684:Magnoliopsida / Q000737:chemistry*; D015394:Molecular Structure; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism*; D010766:Phosphorylation; D018517:Plant Roots / Q000737:chemistry; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism*; D014443:Tyrosine / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D011506:Proteins; D012503:Saponins; D013481:Superoxides; D014443:Tyrosine