An uncoupling protein homologue putatively involved in facultative muscle thermogenesis in birds.
The cDNA of an uncoupling protein (UCP) homologue was obtained by screening a chicken skeletal-muscle library. The predicted 307-amino-acid sequence of avian UCP (avUCP) is 55, 70, 70 and 46% identical with mammalian UCP1, UCP2 and UCP3 and plant UCP respectively. avUCP mRNA expression is restricted to skeletal muscle and its abundance was increased 1.3-fold in a chicken line showing diet-induced thermogenesis, and 3.6- and 2.6-fold in cold-acclimated and glucagon-treated ducklings developing muscle non-shivering thermogenesis respectively. The present data support the implication of avUCP in avian energy expenditure.
Raimbault
S
S
Centre de Recherches sur l'Endocrinologie Moléculaire et le Développement, CNRS, 9 rue Jules Hetzel, F-92190 Meudon, France.
Dridi
S
S
Denjean
F
F
Lachuer
J
J
Couplan
E
E
Bouillaud
F
F
Bordas
A
A
Duchamp
C
C
Taouis
M
M
Ricquier
D
D
D000595:Amino Acid Sequence; D000818:Animals; D030161:Avian Proteins; D001483:Base Sequence; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D002645:Chickens; D017931:DNA Primers; D018076:DNA, Complementary; D024101:Mitochondrial Proteins; D000071245:Mitochondrial Uncoupling Proteins; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D022722:Thermogenesis / Q000502:physiology*
D030161:Avian Proteins; D002352:Carrier Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D024101:Mitochondrial Proteins; D000071245:Mitochondrial Uncoupling Proteins; D012333:RNA, Messenger; C422907:mitochondrial uncoupling protein, chicken