HMGI-C gene expression is not required for in vivo thyroid cell transformation.
We have previously demonstrated that HMGI proteins are required for the transformation of rat thyroid cells by v-mos and v-ras-Ki oncogenes. To determine whether HMGI proteins are also required for in vivo thyroid carcinogenesis, mice carrying a disrupted HMGI-C gene (pygmy mice) were either treated with radioactive iodine or crossed with transgenic mice carrying the E7 papilloma virus oncogene under the transcriptional control of thyroglobulin gene promoter. The pygmy mice developed thyroid carcinomas with the same frequency as occurred in wild-type mice without significant macroscopic and microscopic differences. Therefore, these results indicate that HMGI-C gene expression is not required in in vivo thyroid cell malignant transformation.
Scala
S
S
Dipartimento di Biologia e Patologia Cellulare e Molecolare c/o Centro di Endocrinologia ed Oncologia Sperimentale del CNR, Facoltà di Medicina e Chirurgia di Napoli, Università degli Studi di Napoli 'Federico II', via Pansini, 5, 80131 Naples, Italy.
Portella
G
G
Vitagliano
D
D
Ledent
C
C
Chiappetta
G
G
Giancotti
V
V
Dumont
J
J
Fusco
A
A
D000818:Animals; D015139:Blotting, Southern; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D017931:DNA Primers / Q000737:chemistry; D015870:Gene Expression; D025741:HMGA1a Protein; D006609:High Mobility Group Proteins / Q000096:biosynthesis / Q000235:genetics*; D007124:Immunoenzyme Techniques; D007457:Iodine Radioisotopes; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics*; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000378:metabolism; D050725:Papillomavirus E7 Proteins; D010446:Peptide Fragments; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013961:Thyroid Gland / Q000528:radiation effects / Q000648:ultrastructure; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics*
D017931:DNA Primers; D006609:High Mobility Group Proteins; D007457:Iodine Radioisotopes; D009363:Neoplasm Proteins; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D010446:Peptide Fragments; D014157:Transcription Factors; C059731:oncogene protein E7, Human papillomavirus type 16; D025741:HMGA1a Protein