title: Extremely rapid effects of polyunsaturated fatty acids and N-acetylglucosamine on free-radical metabolism in cultured potato plant cells.
abstract: The effects of arachidonic and linoleic acids, separately and in co-operation with N-acetylglucosamine oligomers, on Solanum tuberosum plant suspension cell cultures were investigated in terms of the fluorescent oxygen-activated-species-sensitive dye 2',7'-dichlorofluorescin diacetate. The inductors used triggered extremely rapid (within 2-10 min) generation of H(2)O(2) in the cells; the majority was expressed in cultures treated with combined polyunsaturated fatty acid and N-acetylglucosamine oligomers. The stimulation of free-radical generation may be related to defensive mechanisms modulating a plant-pathogenic-micro-organism interaction.
authors.affiliation: Polar-Alpine Botanical Garden, Kola Science Centre, 184230 Apatity, Faculty of Biology Moscow State University, 119899 Moscow, Russia. peter_k@oprec.ru
authors.forename: P A
authors.initials: PA
authors.lastname: Kashulin
authors.forename: M N
authors.initials: MN
authors.lastname: Merzlyak
authors.forename: P M
authors.initials: PM
authors.lastname: Zhiboedov
authors.forename: V K
authors.initials: VK
authors.lastname: Zhirov
mesh_terms: D000117:Acetylglucosamine / Q000494:pharmacology*; D016718:Arachidonic Acid / Q000494:pharmacology*; D002470:Cell Survival; D002478:Cells, Cultured; D005452:Fluoresceins; D005456:Fluorescent Dyes; D005609:Free Radicals / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism*; D019787:Linoleic Acid / Q000494:pharmacology*; D011198:Solanum tuberosum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; D005609:Free Radicals; C029569:diacetyldichlorofluorescein; D016718:Arachidonic Acid; D019787:Linoleic Acid; D006861:Hydrogen Peroxide; D000117:Acetylglucosamine