New insights into the mechanisms of lipid-body biogenesis in plants and other organisms.
A comparative approach has been used to study the role of several lipid-body-binding proteins in plants and animals. Caleosins are a newly discovered class of calcium-binding lipid-body proteins found in plants and fungi, which we now report to have separate endoplasmic reticulum- and lipid-body-associated isoforms. We also compare the lipid-body targeting of oleosin from plants and the core protein of the hepatitis C virus when they were expressed separately in lipid-accumulating animal cell lines. This is a novel and powerful approach to investigating the factors that determine the lipid-body targeting of a wide range of proteins.
Murphy
D J
DJ
John Innes Centre, Norwich, U.K.
Hernendez-Pinzon
I
I
Patel
K
K
Hope
R G
RG
McLauchlan
J
J
D000818:Animals; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002460:Cell Line; D005658:Fungi / Q000502:physiology; D050356:Lipid Metabolism; D015388:Organelles / Q000502:physiology*; D018521:Plant Physiological Phenomena; D010940:Plant Proteins / Q000378:metabolism
D002135:Calcium-Binding Proteins; D010940:Plant Proteins; C402836:caleosin protein, Sesamum indicum