title: Effect of 3-hydroxymethylene-2-thioxopyrrolidine on growth of two species of mutans streptococci and their in vitro plaque formation.
abstract: 3-Hydroxymethylene-2-thioxopyrrolidine (HMTP), the major product derived from radish mustard oil, was studied for its activity to inhibit the growth of mutans streptococci, their in vitro plaque formation and their glucan production. The minimum inhibitory concentration (MIC) (800-1600 mg/l) of HMTP at pH 7.0 was reduced to 200 mg/l by lowering the medium pH to 5.0. A dose-dependent inhibition of in vitro plaque formation was observed at 200-800 mg/l dose of HMTP. Production of water-insoluble glucan (WIG) was effectively inhibited by 45-98%, depending on HMTP dose (200-800 mg/l), while only 22% inhibition of water-soluble glucan (WSG) production was observed at an 800 mg/l dose.
authors.affiliation: Department of Bioproductive Sciences, Utsunomiya University, 3218505, Utsunomiya, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hashimoto
authors.forename: K
authors.initials: K
authors.lastname: Yanagi
authors.forename: K
authors.initials: K
authors.lastname: Fukushima
authors.forename: Y
authors.initials: Y
authors.lastname: Uda
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003773:Dental Plaque / Q000382:microbiology*; D005936:Glucans / Q000096:biosynthesis; D005964:Glucosyltransferases / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008826:Microbial Sensitivity Tests; D011759:Pyrrolidines / Q000494:pharmacology*; D012995:Solubility; D013295:Streptococcus mutans / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism
chemical_list: C424262:3-hydroxymethylene-2-thioxopyrrolidine; D000900:Anti-Bacterial Agents; D005936:Glucans; D011759:Pyrrolidines; D005964:Glucosyltransferases; C020025:dextransucrase