Cross-reactivity of antibodies on thymic epithelial cells from humans and marmosets by flow-cytometry.
Callithrix jacchus, the common marmoset, is particularly suitable for immunological studies in vivo and in vitro since many antibodies directed against epitopes of human cells do also react with their analogues from this non-human primate. We studied the reactivity of antibodies against human epitopes on primary cultures of thymic epithelial cells from marmosets and humans by flow-cytometry after different culture periods. The antibodies against integrins, including CD61, reacted with thymic epithelial cells from both humans and marmosets, as did anti-CD44 and anti-CD106. Antibodies specific for thymic epithelial cells (TE-3, TE-4, TE-8, TE-15, TE-16, TE-19) also bound to cells from marmosets but expression of all epitopes was not observed in all cultures studied. The expression of CD51, CD54, CD58 and CD106 on human cells declined after 4 weeks of culture. Our findings indicate that marmosets are a valuable model for immunological studies of effects of xenobiotics on the thymic epithelium.
Riecke
K
K
Institute of Clinical Pharmacology and Toxicology, Department of Toxicology, Benjamin Franklin Medical Centre, Freie Universität Berlin. riecke@medizin.fu-berlin.de
Nogueira
A C
AC
Alexi-Meskishvili
V
V
Stahlmann
R
R
D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000276:immunology; D002144:Callithrix / Q000276:immunology*; D002648:Child; D003429:Cross Reactions; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000276:immunology; D000939:Epitopes; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D016201:Receptors, Lymphocyte Homing / Q000032:analysis / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology*; D015262:Xenobiotics / Q000009:adverse effects
D000906:Antibodies; D000939:Epitopes; D016201:Receptors, Lymphocyte Homing; D015262:Xenobiotics