title: Comparative performance of impactor air samplers for quantification of fungal contamination.
abstract: The aim of this study was to assess the performance of different impactor air samplers for fungal spore collection in the hospital environment. Four recent impactor air samplers were selected: Samplair (AES, Combourg, France); Air Test Omega (LCB, France); Air Samplair Mas-100 (Merck, France); and BioImpactor 100-08 (AES). They were compared with one another at three different hospital sites with varying levels of contaminated air. No significant difference in the efficiency of spore recovery was found between Air Test Omega, Mas-100 and BioImpactor, whereas Samplair was significantly less efficient. BioImpactor was then selected to represent the three superior impactors and was compared with the single-stage Andersen disposable sampler and the Collectron MD8 air sampler (Sartorius, France) and the High Flow Air Sample (BioTest, France), which are based on filtration and centrifugation methods, respectively. No significant difference was observed in terms of spore recovery. On the basis of their performance, unit sampling cost, autonomy and simplicity of use, we conclude that Air Test Omega, Air Samplair Mas-100 and BioImpactor 100-08 are suitable for routine indoor evaluation of fungal contamination of air in hospitals.
authors.affiliation: Laboratoire de Parasitologie-Mycologie, Hôpital Saint-Antoine, France.
authors.forename: D
authors.initials: D
authors.lastname: Nesa
authors.forename: J
authors.initials: J
authors.lastname: Lortholary
authors.forename: A
authors.initials: A
authors.lastname: Bouakline
authors.forename: M
authors.initials: M
authors.lastname: Bordes
authors.forename: J
authors.initials: J
authors.lastname: Chandenier
authors.forename: F
authors.initials: F
authors.lastname: Derouin
authors.forename: J P
authors.initials: JP
authors.lastname: Gangneux
mesh_terms: D000391:Air Microbiology; D002498:Centrifugation / Q000295:instrumentation; D004784:Environmental Monitoring / Q000191:economics / Q000295:instrumentation*; D004867:Equipment Design; D005374:Filtration / Q000295:instrumentation; D005658:Fungi / Q000254:growth & development* / Q000302:isolation & purification*; D006801:Humans; D010297:Paris; D013170:Spores / Q000254:growth & development / Q000302:isolation & purification