The time-course of acid excretion, levels of fluorescence dependent on cellular nicotinamide adenine nucleotide and glycolytic intermediates of Streptococcus mutans cells exposed and not exposed to air in the presence of glucose and sorbitol.
The aim of this study was to examine glucose and sorbitol metabolism in Streptococcus mutans cells exposed and not exposed to air at the coexistence of these compounds by measuring acid excretion, levels of fluorescence dependent on cellular NADH and glycolytic intermediates. An aliquot of bacterial cells grown under strictly anaerobic conditions (anaerobic cells) was exposed temporarily to air (aerobic cells). When glucose was added to the anaerobic cells metabolizing sorbitol, the acid excretion was increased. The level of NADH decreased initially and then increased to the higher plateau level than that during glucose metabolism. The aerobic cells neither metabolized sorbitol nor contained glycolytic intermediates. However, 2 min after glucose was added in the presence of sorbitol, the acid excretion was started slowly and the intermediates appeared. The level of NADH was decreased at first and then increased. These results suggested that the anaerobic S. mutans cells metabolized glucose and sorbitol simultaneously, and that in the presence of sorbitol the aerobic cells could start to metabolize glucose 2 min after glucose was added, as the intermediates (phosphoenopyruvate potential) for the glucose transport were accumulated.
Iwami
Y
Y
Department of Oral Biochemistry, Tohoku University School of Dentistry, Sendai, Japan.
Takahashi-Abbe
S
S
Takahashi
N
N
Yamada
T
T
Kano
N
N
Mayanagi
H
H
D000143:Acids / Q000378:metabolism; D000332:Aerobiosis; D000693:Anaerobiosis; D005453:Fluorescence; D005947:Glucose / Q000032:analysis / Q000378:metabolism*; D006019:Glycolysis; D006801:Humans; D009243:NAD / Q000032:analysis / Q000378:metabolism*; D009249:NADP / Q000032:analysis / Q000378:metabolism; D010100:Oxygen / Q000494:pharmacology*; D010728:Phosphoenolpyruvate / Q000032:analysis / Q000378:metabolism; D013012:Sorbitol / Q000032:analysis / Q000378:metabolism*; D013295:Streptococcus mutans / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D013549:Sweetening Agents / Q000032:analysis / Q000378:metabolism*; D013997:Time Factors
D000143:Acids; D013549:Sweetening Agents; D009243:NAD; D013012:Sorbitol; D009249:NADP; D010728:Phosphoenolpyruvate; D005947:Glucose; D010100:Oxygen