[Polymorphic markers of endothelial NO-synthase and angiotensin II vascular receptor genes and predisposition to ischemic heart disease].
Allele and genotype frequency distribution patterns of the polymorphic regions at the genes for human endothelial NO-synthase (NOS3) (the ecNOS4a/4b VNTR and the Glu298Asp substitution) and the angiotensin II type 1 receptor (AT1)(the A1166C substitution) were compared in 83 unrelated healthy individuals and 88 patients with ischemic heart disease (IHD). In the group of patients statistically significantly higher frequencies of the NOS3 allele 4a (45.5 versus 19.3%), as well as the 4a/4a (15.9 versus 2.4%) and 4a/4b (59.1 versus 33.7%) genotypes were observed. Frequencies of the allele 4b (54.5% versus 80.7%) and the 4b/4b homozygotes (25.0 versus 63.9%) were statistically significantly lower in the group of IHD patients than in healthy individuals. The IHD patients were statistically significantly different from the healthy subjects also in the distributions of the AT1 genotypes. In the former group, a significantly decreased frequency of the AA homozygotes (51.1 versus 65.1%) and an increased frequency of AC heterozygotes (40.9 versus 27.7%) were observed. Thus, in the Moscow population the ecNOS4a/4b VNTR of the NOS3 gene and the A1166C polymorphism of the AT1 gene are associated with the IHD development. Furthermore, the correlation with the IHD revealed was much stronger for the NOS3 VNTR locus.
Chistiakov
D A
DA
State Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, 113545 Russia. dimitry.chistiakov@eudoramail.com
voron'ko
O E
OE
Savost'ianov
K V
KV
Minushkina
L O
LO
D000804:Angiotensin II / Q000378:metabolism*; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D006801:Humans; D017202:Myocardial Ischemia / Q000235:genetics*; D019001:Nitric Oxide Synthase / Q000235:genetics*; D052250:Nitric Oxide Synthase Type III; D011110:Polymorphism, Genetic; D011945:Receptors, Angiotensin / Q000235:genetics* / Q000378:metabolism
D005819:Genetic Markers; D011945:Receptors, Angiotensin; D000804:Angiotensin II; C496314:NOS3 protein, human; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III