title: Frequency of HLA-B alleles in a Caucasoid population determined by a two-stage PCR-SSOP typing strategy.
abstract: High resolution PCR-SSOP typing methods for HLA-B identification have been established and applied to a Northern Ireland population, using large enough numbers to give dependable allele frequencies. The six systems, which operate independently of each other, are intended for use as secondary typing systems following HLA-B identification with a medium resolution PCR-SSOP technique.
authors.affiliation: Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, City Hospital, Belfast, Ireland.
authors.forename: D
authors.initials: D
authors.lastname: Middleton
authors.forename: F
authors.initials: F
authors.lastname: Williams
authors.forename: M A
authors.initials: MA
authors.lastname: Hamill
authors.forename: A
authors.initials: A
authors.lastname: Meenagh
mesh_terms: D000483:Alleles; D001483:Base Sequence; D002461:Cell Line, Transformed; D005787:Gene Frequency / Q000276:immunology*; D015235:HLA-B Antigens / Q000235:genetics*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D009660:Northern Ireland; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012042:Registries; D012984:Software; D044465:White People / Q000235:genetics
chemical_list: D015235:HLA-B Antigens; D015345:Oligonucleotide Probes