Effect of ethanol on uterotropic action of estrogens.
Female rats aging 3 months at the beginning of experiments received 5 or 15% ethanol and then were subjected to bilateral ovariectomy 2 weeks before end of the experiment. During the last 11 days they were daily injected intramuscularly with 2 microg estradiol. Drinking of 5% ethanol combined with injections of estrogens induced DNA damage in the uterus detected by comet assay and abolished induction of progesterone receptors, changes in peroxidase activity, proliferation index, endometrium thickness, and other indices reflecting the hormonal effect of estradiol on the uterus. Drinking of 15% ethanol was accompanied by an increase in DNA-damaging effects of estrogens and a decrease in their hormonal uterotropic effects. It is concluded that unlike tobacco smoking, drinking of moderate ethanol concentrations modifies primarily genotoxic, but not the hormonal effect of estrogens.
Bershtein
L M
LM
N. N. Petrov Institute of Oncology, Ministry of Health of the Russian Federation, St. Petersburg.
Tsyrlina
E V
EV
Poroshina
T E
TE
Gamayunova
V B
VB
Bychkova
N V
NV
Kalinina
N M
NM
Vasil'ev
D A
DA
Kovalenko
I G
IG
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002784:Cholesterol / Q000097:blood; D019363:Cytochrome P-450 CYP1A1; D003577:Cytochrome P-450 Enzyme System / Q000187:drug effects / Q000378:metabolism; D004249:DNA Damage / Q000187:drug effects; D004717:Endometrium / Q000166:cytology / Q000187:drug effects*; D004958:Estradiol / Q000097:blood / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology* / Q000502:physiology; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D011980:Receptors, Progesterone / Q000187:drug effects / Q000378:metabolism; D013250:Steroid Hydroxylases / Q000187:drug effects / Q000378:metabolism; D014599:Uterus / Q000187:drug effects* / Q000254:growth & development
D004967:Estrogens; D011980:Receptors, Progesterone; D000431:Ethanol; D004958:Estradiol; D003577:Cytochrome P-450 Enzyme System; D002784:Cholesterol; D013250:Steroid Hydroxylases; D019363:Cytochrome P-450 CYP1A1; C020120:estrogen 2-hydroxylase