[The modulation effect of granulocyte-colony stimulating factor on the mdr1/P-gp expression in acute myeloid leukemic cells].
OBJECTIVE
To investigate the correlation between cell differentiation and mdr1 gene expression in acute myeloid leukemic(AML) cells.


METHODS
The modulation effect of granulocyte-colony stimulating factor(G-CSF) on the mdr1 gene expression in AML cells was studied in vitro by using polymerase chain reaction(PCR), in vitro cell culture, immunocytochemical and MTT assays.


RESULTS
AML cells retained the ability to respond to the differentiation stimulus. Expressions of P-glycoprotein(P-gp) and mdr1 mRNA were downregulated in differentiated cells, meanwhile, a significant decrease of DNR IC50 in the AML cells with down-expression mdr1 gene was revealed and their sensitivity to anti-cancer drugs increased.


CONCLUSIONS
Leukemic cell differentiation was closely correlated with mdr1 expression. It might be an effective method to overcome drug resistance of leukemic patient by using differentiation inducing agents.
An
J
J
First Affiliated Hospital, Hubei Medical University, Wuhan 430060.
Chen
J
J
Li
X
X
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000096:biosynthesis*; D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D019008:Drug Resistance, Neoplasm; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D019450:Genes, MDR; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D007951:Leukemia, Myeloid / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D016179:Granulocyte Colony-Stimulating Factor