title: Cytogenetic sensitivity of three Fanconi anemia heterozygotes to bleomycin and ionizing radiation.
abstract: It is well known that Fanconi anemia (FA) patients show a hypersensitivity to the effect of cross-linking agents such as mitomycin C and diepoxybutane, while the sensitivity of these patients to ionizing radiation is controversial. Fanconi anemia heterozygotes do not show a hypersensitivity to the above-mentioned agents. However, bleomycin it is used to identify mutagen sensitive individuals, especially among head and neck cancer patients. We present here a preliminary study in which the mean frequencies of bleomycin-induced chromatid breaks (ctb) from three FA heterozygotes (X = 0.90, range 0.80-1.01) and 11 controls (X = 0.40, range 0.21-0.66) differ significantly (P<.001), indicating a high sensitivity to bleomycin of G(2) lymphocytes from these three FA heterozygotes. An increased sensitivity was not observed after exposure of G0 lymphocytes to 2 Gy of ionizing radiation.
authors.affiliation: Unitat d'Antropologia, Dpt. de Biologia Animal, Biologia Vegetal i Ecologia, Facultat de Ciències, Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain. francesc.barquinero@uab.es
authors.forename: J F
authors.initials: JF
authors.lastname: Barquinero
authors.forename: L
authors.initials: L
authors.lastname: Barrios
authors.forename: M
authors.initials: M
authors.lastname: Ribas
authors.forename: J
authors.initials: J
authors.lastname: Egozcue
authors.forename: M R
authors.initials: MR
authors.lastname: Caballín
mesh_terms: D000964:Antimetabolites, Antineoplastic; D001761:Bleomycin; D002842:Chromatids / Q000187:drug effects* / Q000528:radiation effects*; D016001:Confidence Intervals; D004249:DNA Damage; D005199:Fanconi Anemia / Q000235:genetics* / Q000473:pathology; D006579:Heterozygote; D006801:Humans; D007399:Interphase; D008214:Lymphocytes / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects*
chemical_list: D000964:Antimetabolites, Antineoplastic; D001761:Bleomycin