Effect of methyl parathion on the population growth of the rotifer Brachionus patulus (O. F. Müller) under different algal food (Chlorella vulgaris) densities.
The population growth of the rotifer Brachionus patulus was studied under a combination of different concentrations of Chlorella vulgaris and methyl parathion. To obtain sublethal concentrations of the pesticide a 24-h LC(50) bioassay was performed under two food densities (1.5 and 3.0x10(6) cells ml(-1)) using neonate rotifers. For the population growth experiments, seven toxicant concentrations were used ranging from 0.16 to 10.0 mg L(-1) and four food densities (0.75, 1.5, 3.0, and 6.0x10(6) cells ml(-1)), each with four replicates and thus in all, 128 test jars. A medium with Chlorella alone and without toxicant acted as control. The initial density of the rotifers in each replicate were 5 individual ml(-1). Experiments were conducted for 20 days. The LC(50) values of B. patulus were 8.8 and 10.7 mg L(-1) at low and high algal food density, respectively. Data on the population growth studies revealed a significant effect of both food density and toxicant concentration on the maximum abundance, day at which maximum abundance was reached, and rate of population increase per day. At any toxicant concentration, an increase in food density reduced the toxicity of methyl parathion to rotifers. In controls the maximum rotifer density under the highest food level (6x10(6) cells ml(-1)) was 795+/-46 individuals ml(-1). The rate of population increase per day (r) in controls varied from 0.22+/-0.01 to 0.34+/-0.01 depending on food density. Algal food density had a significant influence in reducing the toxicity of methyl parathion to B. patulus under both acute and chronic toxicity test conditions.
Sarma
S S
SS
Division of Biology, National Autonomous University of Mexico, Av. de los Barrios s/n, Los Reyes, AP 314, Tlalnepantla, State of Mexico, CP 54090, Mexico. sarma@servidor.unam.mx
Nandini
S
S
Flores
J L
JL
D000818:Animals; D002708:Chlorella / Q000502:physiology*; D007306:Insecticides / Q000633:toxicity*; D007928:Lethal Dose 50; D008743:Methyl Parathion / Q000633:toxicity*; D011153:Population; D012403:Rotifera / Q000187:drug effects / Q000254:growth & development*
D007306:Insecticides; D008743:Methyl Parathion