Studies on trypsin-modified bovine and human lens acylpeptide hydrolase.
Acylpeptide hydrolase removes the N -acetylated amino acids from the peptide substrates but not from intact proteins. Cleavage between amino acid residues 203--204 of the native acylpeptide hydrolase results in the formation of a 55 kDa truncated active enzyme in the bovine lens, in vivo. In this study we explored the hydrolytic properties of the truncated enzyme using lens beta- and gamma-crystallins as substrates. SDS--PAGE analysis indicated that the beta B2-crystallin was cleaved by truncated acylpeptide hydrolase into several protein fragments (10--26 kDa). No cleavage of the gamma-crystallins was observed under similar conditions. Both the acylpeptide hydrolase activity and the protease activity of the 55 kDa enzyme were completely inhibited by diisopropylfluorophosphate, p -chloromercuribenzoate and ebelactone, and moderately inhibited by N -tosyl phenylalanine chloromethyl ketone. SDS--PAGE analysis followed by fluorography of ((3)H) diisopropylfluorophosphate labeled human lens acylpeptide hydrolase preparation showed the presence of the 55 kDa truncated form of the enzyme, as observed in the bovine lens. The peptide (d)-AIKGDQFL-NH(2)--the amino acid sequence 200--207 of the native bovine acylpeptide hydrolase with an in vivo cleavage site of native protein--was hydrolysed by the lens protease(s) suggesting that the in vivo generation of the 55 kDa acylpeptide hydrolase may be mediated through a proteolytic processing. The protease(s) responsible for the cleavage of this peptide was inhibited by diisopropylfluorophosphate and p -chloromercuribenzoate.
Senthilkumar
R
R
Mason Eye Institute, University of Missouri, One Hospital Drive, Columbia, MO 65212, USA.
Reddy
P N
PN
Sharma
K K
KK
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D002417:Cattle; D002729:Chloromercuribenzoates / Q000494:pharmacology; D003459:Crystallins / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D006868:Hydrolysis; D007531:Isoflurophate / Q000494:pharmacology; D007783:Lactones / Q000494:pharmacology; D007908:Lens, Crystalline / Q000201:enzymology*; D008875:Middle Aged; D010446:Peptide Fragments / Q000737:chemistry; D010447:Peptide Hydrolases / Q000737:chemistry*; D014357:Trypsin / Q000737:chemistry*
D002729:Chloromercuribenzoates; D003459:Crystallins; D004791:Enzyme Inhibitors; D007783:Lactones; D010446:Peptide Fragments; D007531:Isoflurophate; C030794:ebelactone A; D010447:Peptide Hydrolases; D014357:Trypsin