title: Effect of interleukin-1beta on subdiaphragmatic vagal efferents in the rat.
abstract: Interleukin-1beta (IL-1beta) is an important mediator of fever and illness. Recent studies have demonstrated that IL-1beta (2 microg kg(-1)) increases gastric vagal afferent activity. The peripheral mechanisms of the action of lower doses were studied by recording the mass efferent and afferent activity of the gastric branch of the ventral vagal nerve in anesthetized rats. Twenty min after i.v. administration of IL-1beta (1 microg kg(-1)) the efferent activity of the vagal nerve was decreased to 62+/-6% in totally but not in partly vagotomized rats. Preadministration of indomethacin (5 mg kg(-1)) 30 min before IL-1beta blocked this reduction. Administration of 1 microg kg(-1) of IL-1beta had no effect on the afferent activity of the gastric branch of the vagal nerve. The present results suggest that the subdiaphragmatic vagal afferents modulate the parasympathetic efferent outflow in response to IL-1beta partly through prostaglandin dependent mechanisms and that supradiaphragmatic afferents or central sites are more sensitive to the low doses of IL-1beta which becomes evident after elimination of the subdiaphragmatic vagal input.
authors.affiliation: Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden. violeta.bucinskaite@fyfa.ki.se
authors.forename: V
authors.initials: V
authors.lastname: Bucinskaite
authors.forename: M
authors.initials: M
authors.lastname: Kurosawa
authors.forename: T
authors.initials: T
authors.lastname: Lundeberg
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D003964:Diaphragm; D004594:Electrophysiology; D007213:Indomethacin / Q000494:pharmacology; D007275:Injections, Intravenous; D007375:Interleukin-1 / Q000494:pharmacology*; D008297:Male; D009475:Neurons, Afferent / Q000187:drug effects; D009476:Neurons, Efferent / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013268:Stimulation, Chemical; D014628:Vagotomy; D014630:Vagus Nerve / Q000166:cytology* / Q000187:drug effects* / Q000601:surgery
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D007375:Interleukin-1; D007213:Indomethacin