[The evidence for existence of interleukin 8 inhibitor and its biological activity in gingival crevicular fluid].
In order to confirm the existence of Interleukin-8 (IL-8) inhibitor and its biological activity in gingival crevicular fluid (GCF), this study examined the GCF taken from 7 adult periodontitis (AP) patients. In neutralization test of IL-8, the results showed that the mean level of IL-8 was less than 1 ng/ml, which had been added into the GCF before ELESAs was performed to measure the amount of IL-8 in GCF. The mean level of IL-8 in the GCF of AP group was significantly lower than that of healthy group (P < 0.001). In biological activity test of IL-8 inhibitor, using pooled GCF taken from 8 AP patients (23 teeth), the results showed that the GCF (without recombinant human IL-8, rhIL-8) caused more white blood cell (WBC) migration than blank control group (physiological saline) did. When the amount of rhIL-8 increased in GCF from 0.1 microgram to 1 microgram, the WBC count increased by 18.6% which was less than the increase rate (49.1%) in control group with same dose of IL-8. In the saline group containing rhIL-8, the WBC chemotactic response appeared as an inverted "V"-shape curve. All these data suggested that 1. Certain kinds of IL-8 inhibitor exist in GCF which can "cleave" IL-8. 2. The level of IL-8 inhibitor(s) increases significantly in the GCF from periodontitis sites. 3. The GCF of adult periodontitis patient has strong chemotactic effects on WBC. IL-8 inhibitor(s) in GCF can slightly suppress the chemotactic effect induced by IL-8. When assessing the role of IL-8 in pathophysiology, the high and low dose of IL-8 might have different sense.
Xie
H
H
School of Stomatology, Hubei Medical University, Wuhan 430070.
Cao
C
C
Ma
D
D
D000328:Adult; D002634:Chemotaxis, Leukocyte; D005883:Gingival Crevicular Fluid / Q000378:metabolism*; D006801:Humans; D016209:Interleukin-8 / Q000037:antagonists & inhibitors* / Q000378:metabolism / Q000494:pharmacology; D010518:Periodontitis / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology
D016209:Interleukin-8; D011994:Recombinant Proteins