Effect of vagal autotransplantation on quantitative [3H]-vesamicol binding image in rats with unilateral lesions of nucleus baslis magnocellularis.
It is considered that nodosal ganglion grafting improves learning and memory disorder in nucleus basalis magnocellularis lesioned rats, and that [3H]-vesamicol is one of the mapping agents for presynaptic cholinergic neurons. Quantitative autoradiography was performed using high sensitive imaging plate system to evaluate the effect of neurotransplantation on [3H]-vesamicol binding. The affected-side to unaffected-side ratio of [3H]-vesamicol in the nucleus basalis magnocellularis lesioned group was significantly lower than that in the sham-operated group at 1, 2 and 4weeks (P<0.01). In the transplanted group, the ratio was significantly higher than that in the untreated group at 4weeks (P<0.05). [3H]-vesamicol binding is one of the most sensitive indicators of nucleus basalis magnocellularis lesions and the effect of the nodosal ganglion grafting.
Ikeda
E
E
Department of Nuclear Medicine, Kanazawa University School of Medicine, 13-1 Takara-machi, 920-8640, Kanazawa, Japan. eikeda1@mac.com
Ichikawa
A
A
Mori
H
H
Shiba
K
K
Kuji
I
I
Sumiya
H
H
Tonami
N
N
Ikeda
K
K
D000818:Animals; D001345:Autoradiography; D020532:Basal Nucleus of Meynert / Q000033:anatomy & histology / Q000378:metabolism*; D007091:Image Processing, Computer-Assisted; D008297:Male; D009467:Neuromuscular Depolarizing Agents / Q000378:metabolism*; D010880:Piperidines / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014182:Transplantation, Autologous / Q000502:physiology*; D014630:Vagus Nerve / Q000637:transplantation*
D009467:Neuromuscular Depolarizing Agents; D010880:Piperidines; C006189:vesamicol