title: Transcription inhibition induced by modified triple helix-forming oligonucleotides: a quantitative assay for evaluation in cells.
abstract: Oligonucleotides can bind to double-stranded DNA in a sequence-specific manner to form triple helices. Uniformly modified, pyrimidine-rich oligodeoxyribonuclotides containing internucleosidic N3'-P5' phosphoramidate linkages are known to form very stable triplexes with their DNA target. Psoralen-conjugated triple helix-forming oligonucleotides (Pso-TFOs) can additionally be photo-induced to become irreversibly bound to their targeted DNA sequence. Here, we have examined the ability of various 15-mer phosphoramidate TFOs targeted to the HIV-1 polypurine tract (PPT) sequence to prevent transcription elongation in cell cultures; the PPT sequence has been cloned in the transcribed region of a reporter firefly luciferase gene (luc) and transient expression experiments performed. We show that the level of transcription inhibition of the reporter gene in cells perfectly correlates with the amount of covalent triplex at the PPT site. The efficacy of non-covalent triplexes (either omitting the irradiation step with the psoralen conjugate, or using the unsubstituted oligonucleotide) has been studied in our expression system; the oligonucleotides were introduced into living cells by cationic lipid-mediated delivery or directly into the cell nucleus by microinjection. This experimental approach allowed us to evaluate the intrinsic activity of triplexes as transcriptional inhibitors; transcription elongation was inhibited in cells in a sequence-dependent and concentration-dependent manner. This experimental system is convenient for quantitative and fast evaluation of new chemistries of antigene oligonucleotides as inhibitors of gene expression in cells and in vivo.
authors.affiliation: Laboratoire de Biophysique Muséum National d'Histoire Naturelle, INSERM U.201 - CNRS UMR 8646, 43 rue Cuvier, Paris, 75005, France.
authors.forename: M
authors.initials: M
authors.lastname: Faria
authors.forename: C D
authors.initials: CD
authors.lastname: Wood
authors.forename: M R
authors.initials: MR
authors.lastname: White
authors.forename: C
authors.initials: C
authors.lastname: Hélène
authors.forename: C
authors.initials: C
authors.lastname: Giovannangeli
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D015967:Gene Expression Regulation, Viral / Q000187:drug effects; D017930:Genes, Reporter / Q000235:genetics; D015497:HIV-1 / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D008845:Microinjections; D009691:Nucleic Acid Denaturation; D009841:Oligonucleotides / Q000008:administration & dosage / Q000235:genetics* / Q000494:pharmacology; D013379:Substrate Specificity; D013696:Temperature; D014158:Transcription, Genetic / Q000187:drug effects / Q000235:genetics*; D014162:Transfection
chemical_list: D009841:Oligonucleotides; C051802:triplex DNA; D004247:DNA