Localization of the e-NOS enzyme in endothelial cells and odontoblasts of healthy human dental pulp.
Nitric oxide synthases (NOS) are important enzymes present in different cells such as endothelial cells, macrophages, etc. Recently, it has been found that nitric oxide (NO) is responsible for vasodilation, blood pressure regulation, platelet aggregation, cardiac contractility, and the mediation of immunity during bacterial infections and inflammation. However, the production and role of NO in various structures of the oral cavity have not been investigated extensively. The aim of this study was to evaluate the presence of e-NOS in healthy human odontoblasts and endothelial cells of the dental pulp. Twenty healthy human dental pulps were collected and frozen and pulp slices were obtained using a cryostat. The e-NOS enzyme was revealed by immunohistochemical analysis and the enzyme level was detected by Western blotting and mRNA expression by RT-PCR. The immunohistochemical results demonstrated, for the first time, the presence of e-NOS in odontoblasts and in endothelial cells. The presence of e-NOS m-RNA was confirmed by RT-PCR and the expression of the protein by Western blotting. These results clearly show that the e-NOS enzyme is present in both odontoblasts and endothelial cells of healthy human pulp. The presence of e-NOS in the odontoblast and endothelial cells of the dental pulp may mediate local vasodilation and cell proliferation.
Felaco
M
M
School of Dentistry, University of Chieti, Italy.
Di Maio
F D
FD
De Fazio
P
P
D'Arcangelo
C
C
De Lutiis
M A
MA
Varvara
G
G
Grilli
A
A
Barbacane
R C
RC
Reale
M
M
Conti
P
P
D015153:Blotting, Western; D003782:Dental Pulp / Q000098:blood supply / Q000166:cytology / Q000201:enzymology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000201:enzymology*; D006801:Humans; D007124:Immunoenzyme Techniques; D019001:Nitric Oxide Synthase / Q000235:genetics / Q000378:metabolism*; D052250:Nitric Oxide Synthase Type III; D009804:Odontoblasts / Q000166:cytology / Q000201:enzymology*; D012313:RNA / Q000032:analysis; D012333:RNA, Messenger; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger; D012313:RNA; C496314:NOS3 protein, human; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III