Plasmodium falciparum: histidine-rich protein II is expressed during gametocyte development.
Both early gametocytes (I-II) and asexual trophozoite stages of Plasmodium falciparum digest hemoglobin and detoxify haem by polymerizing it into parasite pigment called hemozoin. The mechanism of polymerization is unclear but it has been proposed that histidine-rich protein II may facilitate transport of hemoglobin to the food vacuole and catalyze the polymerization in asexual stages. We describe the transcription of histidine-rich protein II in gametocytes by Northern blot analysis and the expression of the protein in these stages by immunoprecipitation and Western blotting. Localization of histidine-rich protein II within the gametocyte by immunofluorescence assay and immunoelectron microscopy clearly illustrated the presence of this molecule in the infected red cell cytosol in the early stages of gametocyte development and internalization in the later gametocyte as it matures. There is a strong correlation between the stage-specific trafficking of histidine-rich protein II in gametocytes and the susceptibility of early but not late gametocytes to the antimalarial drug chloroquine.
Hayward
R E
RE
Wellcome Trust Centre for the Epidemiology of Infectious Disease, Department of Zoology, University of Oxford, South Parks Road, Oxford, OX1 3PS, United Kingdom.
Sullivan
D J
DJ
Day
K P
KP
D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D019084:Fluorescent Antibody Technique, Indirect; D018507:Gene Expression Regulation, Developmental; D006454:Hemoglobins / Q000378:metabolism; D016253:Microscopy, Immunoelectron; D010963:Plasmodium falciparum / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis / Q000235:genetics; D015800:Protozoan Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics; D014158:Transcription, Genetic; D000099053:Histidine-Rich Glycoprotein
D006454:Hemoglobins; D011506:Proteins; D015800:Protozoan Proteins; D000099053:Histidine-Rich Glycoprotein