title: Vestibular efferent neurons project to the flocculus.
abstract: A bilateral projection from the vestibular efferent neurons, located dorsal to the genu of the facial nerve, to the cerebellar flocculus and ventral paraflocculus was demonstrated. Efferent neurons were double-labeled by the unilateral injections of separate retrograde tracers into the labyrinth and into the floccular and ventral parafloccular lobules. Efferent neurons were found with double retrograde tracer labeling both ipsilateral and contralateral to the sites of injection. No double labeling was found when using a fluorescent tracer with non-fluorescent tracers such as horseradish peroxidase (HRP) or biotinylated dextran amine (BDA), but large percentages of efferent neurons were found to be double labeled when using two fluorescent substances including: fluorogold, microruby dextran amine, or rhodamine labeled latex beads. These data suggest a potential role for vestibular efferent neurons in modulating the dynamics of the vestibulo-ocular reflex (VOR) during normal and adaptive conditions.
authors.affiliation: University of Texas Medical Branch, Department of Otolaryngology, 7.102 MRB, Galveston, TX 77555-1063, USA.
authors.forename: M E
authors.initials: ME
authors.lastname: Shinder
authors.forename: I M
authors.initials: IM
authors.lastname: Purcell
authors.forename: G D
authors.initials: GD
authors.lastname: Kaufman
authors.forename: A A
authors.initials: AA
authors.lastname: Perachio
mesh_terms: D000818:Animals; D001710:Biotin; D002529:Cerebellar Nuclei / Q000166:cytology / Q000502:physiology*; D004525:Efferent Pathways / Q000502:physiology; D005260:Female; D005456:Fluorescent Dyes; D007839:Functional Laterality / Q000502:physiology; D005849:Gerbillinae; D006651:Histocytochemistry; D006735:Horseradish Peroxidase; D008297:Male; D009476:Neurons, Efferent / Q000502:physiology*; D012027:Reflex, Vestibulo-Ocular / Q000502:physiology; D013266:Stilbamidines; D014722:Vestibule, Labyrinth / Q000166:cytology / Q000502:physiology*
chemical_list: C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D005456:Fluorescent Dyes; D013266:Stilbamidines; D001710:Biotin; D006735:Horseradish Peroxidase