title: Temporally and spatially regulated expression of a candidate G-protein-coupled receptor during cerebral cortical development.
abstract: Genes expressed in layer-specific patterns in the mammalian cerebral cortex may play a role in specifying the identity of different cortical layers. Using PCR-differential display, we identified a cDNA that encodes rCNL3, a gene cloned previously by sequence homology to G-protein-coupled receptors. rCNL3 is expressed predominantly in layers 2-4 of the young rat cortex and in the developing and adult striatum. Cortical expression of rCNL3 begins postnatally at P3 and continues at high levels until around P15, while striatal expression begins at E20 and continues through adulthood. rCNL3 expression is not detectable in the ventricular zone precursors that generate the neurons of layers 2-4. The expression pattern of rCNL3 in the developing cortex suggests that rCNL3 is not involved in the initial specification of laminar fate, but rather may be involved with later differentiation events within the superficial cortical layers.
authors.affiliation: Department of Biological Sciences, Stanford University, 385 Serra Mall, Stanford, California 94305, USA.
authors.forename: A
authors.initials: A
authors.lastname: Chenn
authors.forename: M E
authors.initials: ME
authors.lastname: Levin
authors.forename: S K
authors.initials: SK
authors.lastname: McConnell
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D019521:Body Patterning / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D002540:Cerebral Cortex / Q000166:cytology / Q000196:embryology* / Q000254:growth & development*; D019976:Cloning, Organism / Q000379:methods; D003624:Darkness; D005333:Fetus; D019204:GTP-Binding Proteins / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism; D051381:Rats; D011956:Receptors, Cell Surface / Q000235:genetics*; D043562:Receptors, G-Protein-Coupled; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*
chemical_list: C427404:Gpr6 protein, rat; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D019204:GTP-Binding Proteins