title: Crystallization and preliminary X-ray diffraction analysis of the chloramphenicol acetyltransferase from Tn2424.
abstract: Crystals of chloramphenicol acetyltransferase B2, an enzyme encoded by the transposon Tn2424 from Escherichia coli, have been obtained utilizing polyethylene glycol as a precipitant. The enzyme inactivates the antibiotic chloramphenicol and is a member of the xenobiotic acetyltransferase family. Two crystal forms were obtained and complete data sets have been collected at a synchrotron source: form I, which diffracted to 3.2 A, and form II, grown in the presence of NiCl(2), for which crystals of the apoenzyme and of the enzyme-chloramphenicol complex have been obtained. For the form II crystals, complete data sets have been collected at 2.7 and 3.2 A resolution, respectively. The space group of the above two crystal forms is P2(1)3, with unit-cell parameter a = 130 A.
authors.affiliation: Molecular Endocrinology and Oncology Laboratory, CHUL Research Center and Laval University, Quebec, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Zhou
authors.forename: M L
authors.initials: ML
authors.lastname: Lu
authors.forename: W
authors.initials: W
authors.lastname: Qiu
authors.forename: R L
authors.initials: RL
authors.lastname: Campbell
authors.forename: V
authors.initials: V
authors.lastname: Nahoum
authors.forename: J
authors.initials: J
authors.lastname: Lapointe
authors.forename: P H
authors.initials: PH
authors.lastname: Roy
authors.forename: S X
authors.initials: SX
authors.lastname: Lin
mesh_terms: D001051:Apoenzymes / Q000737:chemistry / Q000302:isolation & purification; D015500:Chloramphenicol O-Acetyltransferase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D003460:Crystallization; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D011092:Polyethylene Glycols; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D014961:X-Ray Diffraction
chemical_list: D001051:Apoenzymes; D004251:DNA Transposable Elements; D011994:Recombinant Proteins; D011092:Polyethylene Glycols; D015500:Chloramphenicol O-Acetyltransferase