Atomic resolution structure of a mutant of the spectrin SH3 domain.
The crystal structure of an alpha-spectrin Src-homology 3 (SH3) domain mutant has been refined at 1.12 A resolution. This X-ray structure is at the highest resolution achieved so far for an SH3 domain. The structure allows the identification of a complete set of specific interactions and is useful for the elucidation of relations between structure and pH-dependent thermodynamic stability in a series of SH3 domain mutants.
Berisio
R
R
Centro di Studio di Biocristallografia, CNR and Dipartimento di Chimica, Università di Napoli Federico II, Via Mezzocannone 4, I-80134 Napoli, Italy. rberisio@chemistry.unina.it
Viguera
A
A
Serrano
L
L
Wilmanns
M
M
D003460:Crystallization; D018360:Crystallography, X-Ray; D006860:Hydrogen Bonding; D008958:Models, Molecular; D009154:Mutation; D017433:Protein Structure, Secondary; D012680:Sensitivity and Specificity; D013049:Spectrin / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D018909:src Homology Domains
D013049:Spectrin