title: Cellular uptake and cytotoxicity of solid lipid nanospheres (SLN) incorporating doxorubicin or paclitaxel.
abstract: Solid Lipid Nanospheres (SLN) are colloidal therapeutic systems proposed for several administration routes and obtained by dispersing warm microemulsions in cold water. SLN as carriers of doxorubicin and paclitaxel have been previously studied. In this study, the cellular uptake of SLN and the cytotoxicity of doxorubicin and paclitaxel incorporated into SLN were investigated on two cell-lines, human promyelocytic leukemia (HL60) and human breast carcinoma (MCF-7). Cellular uptake of SLN was determined by incorporating 6-coumarin as fluorescent marker. The cellular uptake of fluorescent SLN was clearly evidenced by fluorescence microscopy. The cytotoxicity of doxorubicin incorporated in SLN was higher compared to the conventional doxorubicin solution, even at the lower concentrations. Paclitaxel in SLN was about 100-fold more effective than free paclitaxel on MCF-7 cells, while on HL60 cells a lower sensitivity was achieved with paclitaxel in SLN. Unloaded SLN had no cytotoxic effect on HL60 and MCF-7 cells.
authors.affiliation: Dipartimento di Medicina ed Oncologia Sperimentale, Università degli Studi di Torino, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Miglietta
authors.forename: R
authors.initials: R
authors.lastname: Cavalli
authors.forename: C
authors.initials: C
authors.lastname: Bocca
authors.forename: L
authors.initials: L
authors.lastname: Gabriel
authors.forename: M R
authors.initials: MR
authors.lastname: Gasco
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000378:metabolism* / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000378:metabolism* / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D004317:Doxorubicin / Q000378:metabolism* / Q000494:pharmacology*; D005456:Fluorescent Dyes; D018922:HL-60 Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D008863:Microspheres; D017239:Paclitaxel / Q000378:metabolism* / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D000903:Antibiotics, Antineoplastic; D000972:Antineoplastic Agents, Phytogenic; D005456:Fluorescent Dyes; D004317:Doxorubicin; D017239:Paclitaxel