Blood group genotyping in a population of highly diverse ancestry.
Accurate phenotyping of red blood cells (RBCs) can be difficult in transfusion-dependent patients such as those with thalassemia and sickle cell anemia because of the presence of previously transfused RBCs in the patient's circulation. Recently, the molecular basis associated with the expression of many blood group antigens was established. This allowed the development of a plethora of polymerase chain reaction (PCR)-based tests for identification of the blood group antigens by testing DNA. The new technologies complement phenotyping and overcome some of the limitations of hemagglutination assays. These molecular assays were developed on the basis of DNA sequences of individuals of Caucasian ancestry. The present study addresses the concern that these genotyping assays may not be applicable to populations of highly diverse ancestry because of variability in intronic regions or because of unrecognized alleles. We determined both phenotype and genotype for RH D, K 1/K 2, JK A/JK B, FY A/ FY B-GATA in 250 normal blood donors using PCR. Phenotype and genotype results agreed in 100% of the cases, indicating that molecular genotyping protocols can be effectively applied to populations with a highly diverse genetic background. However, genotyping for Duffy antigens provided information that could not be obtained by phenotyping. Essentially, 30.5 % of the donors with the FY B gene typed as Fy(b-) because of mutations in the GATA box. This information is very useful for the management of transfusion dependent patients.
Pellegrino
J
J
Hemocentro Unicamp, Campinas, SP, Brazil. jordao@mpcnet.com.br
Castilho
L
L
Rios
M
M
De Souza
C A
CA
D001782:Blood Donors; D001789:Blood Group Antigens / Q000235:genetics*; D001803:Blood Transfusion; D004375:Duffy Blood-Group System / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005091:Exons; D005838:Genotype; D006801:Humans; D007438:Introns; D007626:Kell Blood-Group System / Q000235:genetics; D007667:Kidd Blood-Group System / Q000235:genetics; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012204:Rh-Hr Blood-Group System / Q000235:genetics; D044465:White People / Q000235:genetics
D001789:Blood Group Antigens; D004375:Duffy Blood-Group System; D007626:Kell Blood-Group System; D007667:Kidd Blood-Group System; D012204:Rh-Hr Blood-Group System