Functional properties of circulating and transmigrated neutrophils in a rat peritonitis model.
Using a rat peritonitis model, we examined the pattern of expression of adhesion molecules and H2O2 productivity of neutrophils by indirect immunofluorescence and flow cytometry. H2O2 production in blood neutrophils was significantly increased after peritonitis induction. The expression of CD11b and CD18 antigens on blood neutrophils was also significantly increased after peritonitis induction. In contrast, H2O2 production in peritoneal neutrophils was significantly lower than that in blood neutrophils. The expression of CD11b and CD45 antigens on peritoneal neutrophils was significantly higher than on blood neutrophils. Although no function has been assigned to LFA-1 on neutrophils, the expression of LFA-1 (CD11a/CD18) on peritoneal neutrophils was significantly decreased.
Mori
T
T
Second Department of Surgery, Kansai Medical University, Osaka, Japan. phil@ma.kcom.ne.jp
Masuda
M
M
Takahashi
H
H
Hioki
K
K
D000818:Animals; D018821:CD18 Antigens / Q000378:metabolism; D004195:Disease Models, Animal; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D006861:Hydrogen Peroxide / Q000378:metabolism; D016177:Macrophage-1 Antigen / Q000378:metabolism; D008297:Male; D009504:Neutrophils / Q000276:immunology / Q000473:pathology / Q000502:physiology*; D010529:Peritoneal Cavity / Q000473:pathology; D010538:Peritonitis / Q000097:blood* / Q000473:pathology / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar
D018821:CD18 Antigens; D016177:Macrophage-1 Antigen; D006861:Hydrogen Peroxide