title: Duration of the protection of an E2 subunit marker vaccine against classical swine fever after a single vaccination.
abstract: The period during which pigs are protected after vaccination is important for the successful usage of a marker vaccine against classical swine fever virus (CSFV) in an eradication programme. In four animal experiments with different vaccination-challenge intervals we determined the duration of protection of an E2 subunit marker vaccine in pigs after a single vaccination. Unvaccinated pigs were included in each group to detect transmission of the challenge virus. Three groups of six pigs were vaccinated once and subsequently inoculated with the virulent CSFV strain Brescia after a vaccination-challenge interval of 3, 51/2, 6 or 13 months. All vaccinated pigs, 16 out of 18, with neutralising antibodies against CSFV at the moment of challenge, 3, 51/2, 6 or 13 months later, survived, whereas unvaccinated control pigs died from acute CSF or were killed being moribund. A proportion of the vaccinated pigs did however develop fever or cytopenia after challenge and two vaccinated pigs were viremic after challenge. Virus transmission of vaccinated and challenged pigs to unvaccinated sentinel pigs did not occur in groups of pigs which were challenged 3 or 6 months after a single vaccination. Two out of eight vaccinated pigs that were found negative for CSFV neutralising antibody at 13 months after vaccination died after subsequent challenge. The findings in this study demonstrate that pigs can be protected against a lethal challenge of CSFV for up to 13 months after a single vaccination with an E2 subunit marker vaccine.
authors.affiliation: Department of Mammalian Virology, Institute for Animal Science and Health (ID-Lelystad), PO Box 65, 8200 AB, Lelystad, The Netherlands.
authors.forename: A J
authors.initials: AJ
authors.lastname: de Smit
authors.forename: A
authors.initials: A
authors.lastname: Bouma
authors.forename: E P
authors.initials: EP
authors.lastname: de Kluijver
authors.forename: C
authors.initials: C
authors.lastname: Terpstra
authors.forename: R J
authors.initials: RJ
authors.lastname: Moormann
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D006691:Classical Swine Fever / Q000276:immunology* / Q000517:prevention & control / Q000635:transmission; D006692:Classical Swine Fever Virus / Q000276:immunology* / Q000472:pathogenicity; D019085:Fluorescent Antibody Technique, Direct / Q000662:veterinary; D007958:Leukocyte Count / Q000662:veterinary; D009500:Neutralization Tests / Q000662:veterinary; D011897:Random Allocation; D013552:Swine; D013921:Thrombocytopenia / Q000662:veterinary; D013997:Time Factors; D014611:Vaccination / Q000662:veterinary*; D014759:Viral Envelope Proteins / Q000276:immunology*; D014766:Viremia / Q000662:veterinary
chemical_list: D000914:Antibodies, Viral; D014759:Viral Envelope Proteins; C087086:glycoprotein E2, classical swine fever virus