Development of an ELISA for the diagnosis of Corynebacterium pseudotuberculosis infections in goats.
An ELISA was developed for the diagnosis of Corynebacterium pseudotuberculosis infections in goats. A bacterial whole cell extract was used as solid-phase antigen, and serum from a culture-positive animal served as the internal reference standard. The well-to-well and assay-to-assay variations were determined to be 12.7 and 33.0%, respectively. A cut off value was determined by parallel testing of 142 sera (112 ELISA-positive, 30 ELISA-negative) in a Western blot, and the correlation between both tests was highly significant (K=0, 93). In addition, the reliability of the ELISA for the detection of infected herds was proven in a double blind study testing 910 sera from 74 goat herds.
Kaba
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J
Katedra Chorob Zakaznych, Mikrobiologii i Parazytologii, Wydzial Medycyny Weterynaryjnej SGGW, Grochowska 272, 03-849, Warszawa, Poland. kaba@amaltea.sggw.waw.pl
Kutschke
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L
Gerlach
G F
GF
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial; D015153:Blotting, Western / Q000662:veterinary; D003354:Corynebacterium Infections / Q000097:blood / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D016925:Corynebacterium pseudotuberculosis / Q000302:isolation & purification*; D003931:Diacetyl; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005260:Female; D015511:Goat Diseases / Q000097:blood / Q000175:diagnosis / Q000382:microbiology*; D006041:Goats; D012372:ROC Curve; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D003931:Diacetyl