A new in vivo and in vitro B cell lymphoma model, pi-BCL1.
We report a new variant of the BCL1 syngeneic mouse B-cell lymphoma model, which we have called pi-BCL1. pi-BCL1 can be established as a syngeneic tumor in BALB/c mice. Tumors can be removed, prepared and easily grown in liquid culture and subsequently transferred back successfully as syngeneic tumors. As a syngeneic tumor pi-BCL1 behave more like a lymphoma with solid tumor masses, than a chronic lymphocytic leukaemia of the original BCL1 model. The immunophenotype and the growth characteristics of the pi-BCL1 and BCL1 tumors appear very similar. Cytologically, pi-BCL1 appears to be a transformation from a small lymphocytic lymphoma to a more diffuse large cell centroblast-like higher-grade lymphoma. We are not aware of any previous reports of such transformation events in a syngeneic animal model of B cell lymphoma. We believe pi-BCL1 provides a useful new tool for the study of B cell lymphoma in vitro and in vivo and enables reduced numbers of tumor passage in mice.
Illidge
T
T
Cancer Sciences Division, School of Medicine, Southampton University, Southampton, United Kingdom. tmi@soton.ac.uk
Honeychurch
J
J
Howatt
W
W
Ross
F
F
Wilkins
B
B
Cragg
M
M
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D004273:DNA, Neoplasm / Q000032:analysis; D004195:Disease Models, Animal; D005260:Female; D005455:Fluorescent Antibody Technique; D016130:Immunophenotyping; D066298:In Vitro Techniques; D007621:Karyotyping; D008099:Liver / Q000166:cytology / Q000473:pathology; D016393:Lymphoma, B-Cell / Q000473:pathology*; D008224:Lymphoma, Follicular / Q000473:pathology / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000166:cytology
D000954:Antigens, Surface; D004273:DNA, Neoplasm