A complex composed of USF1 and USF2 activates the human FcepsilonRI alpha chain expression via a CAGCTG element in the first intron.
The high-affinity IgE receptor, FcepsilonRI, is a key regulatory molecule in the allergic reaction. During the course of studies to find cis-acting elements for FcepsilonRI alpha chain gene expression, a CAGCTG sequence located in the first intron was revealed to serve as a crucial enhancer element. Electromobility shift assays using antibodies and in vitro translation products showed that the CAGCTG element was recognized by the USF1/USF2 complex. As was the case for other intronic cis-elements, the CAGCTG element regulated the promoter in an orientation- and position-dependent manner. Overexpression of USF2 antisense repressed the FcepsilonRI alpha chain gene promoter and decreased the amount of alpha chain mRNA in mast cell lines. All these results indicated that the USF1/USF2 complex activates the human FcepsilonRI alpha chain gene expression via the CAGCTG element in the first intron.
Takahashi
K
K
Foods & Pharmaceuticals Research & Development Laboratory, Asahi Breweries Ltd., Ibaraki, Japan. Kyoko.takahashi@asahibeer.co.jp
Nishiyama
C
C
Nishiyama
M
M
Okumura
K
K
Ra
C
C
Ohtake
Y
Y
Yokota
T
T
D000818:Animals; D016375:Antisense Elements (Genetics) / Q000494:pharmacology; D002478:Cells, Cultured; D004268:DNA-Binding Proteins; D019281:Dimerization; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D006801:Humans; D007438:Introns; D051381:Rats; D017455:Receptors, IgE / Q000235:genetics*; D014157:Transcription Factors / Q000737:chemistry / Q000502:physiology*; D014162:Transfection; D051720:Upstream Stimulatory Factors
D016375:Antisense Elements (Genetics); D004268:DNA-Binding Proteins; D017455:Receptors, IgE; D014157:Transcription Factors; C497181:USF1 protein, human; C497184:USF2 protein, human; D051720:Upstream Stimulatory Factors; C497182:Usf1 protein, rat; C497187:Usf2 protein, rat