Upregulation of HSP47 and collagen type III in the dermal fibrotic disease, keloid.
Keloid is a dermal fibrotic disease characterized by excessive accumulation of mainly type I collagen in extracellular matrix of the dermis. We have studied the expression levels of collagen types I and III, and its molecular chaperone HSP47 in keloid lesions and surrounding unaffected skin using Northern and Western blotting and immunohistochemical analyses. Collagen types I and III mRNA levels were found to be upregulated 20-fold in keloid tissues, contradicting previous reports of nearly normal type III collagen levels in this disease. HSP47 expression in keloid lesions was also highly upregulated; eightfold at mRNA level and more than 16-fold at the protein level. Strong upregulation of these three proteins in keloid was confirmed by immunohistochemical staining. These results suggest that accumulation of both type I and type III collagen is important for the development of keloid lesions, and that HSP47 plays a role in the rapid and extensive synthesis of collagen in keloid tissues.
Naitoh
M
M
Department of Molecular and Cellular Biology, Kyoto University, Kyoto, 606-8397, Japan.
Hosokawa
N
N
Kubota
H
H
Tanaka
T
T
Shirane
H
H
Sawada
M
M
Nishimura
Y
Y
Nagata
K
K
D000293:Adolescent; D000328:Adult; D000368:Aged; D003094:Collagen / Q000032:analysis / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D050958:HSP47 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000032:analysis / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D007627:Keloid / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
D050958:HSP47 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D012333:RNA, Messenger; C494660:SERPINH1 protein, human; D003094:Collagen