title: Comparison of neurologic outcome after deep hypothermic circulatory arrest with alpha-stat and pH-stat cardiopulmonary bypass in newborn pigs.
abstract: OBJECTIVE
  Deep hypothermic circulatory arrest for neonatal heart surgery poses the risk of brain damage. Several studies suggest that pH-stat management during cardiopulmonary bypass improves neurologic outcome compared with alpha-stat management. This study compared neurologic outcome in a survival piglet model of deep hypothermic circulatory arrest between alpha-stat and pH-stat cardiopulmonary bypass.
  
  
  METHODS
  Piglets were randomly assigned to alpha-stat (n = 7) or pH-stat (n = 7) cardiopulmonary bypass, cooled to 19 degrees C brain temperature, and subjected to 90 minutes of deep hypothermic circulatory arrest. After bypass rewarming/reperfusion, they survived 2 days. Neurologic outcome was assessed by neurologic performance (0-95, 0 = no deficit and 95 = brain death) and functional disability scores, as well as histopathology. Arterial pressure, blood gas, glucose, and brain temperature were recorded before, during, and after bypass.
  
  
  RESULTS
  All physiologic data during cardiopulmonary bypass were similar between groups (pH-stat vs alpha-stat) except arterial pH (7.06 +/- 0.03 vs 7.43 +/- 0.09, P <.001) and arterial PCO (2) (98 +/- 8 vs 36 +/- 8 mm Hg, P <.001). No differences existed in duration of cardiopulmonary bypass or time to extubation. Performance was better in pH-stat versus alpha-stat management at 24 hours (2 +/- 3 vs 29 +/- 17, P = 0.004) and 48 hours (1 +/- 2 vs 8 +/- 9, P =.1). Also, functional disability was less severe with pH-stat management at 24 hours (P =.002) and 48 hours (P =.053). Neuronal cell damage was less severe with pH-stat versus alpha-stat in the neocortex (4% +/- 2% vs 15% +/- 7%, P <.001) and hippocampal CA1 region (11% +/- 5% vs 33% +/- 25%, P =.04), but not in the hippocampal CA3 region (3% +/- 5% vs 16% +/- 23%, P =.18) or dentate gyrus (1% +/- 1% vs 3% +/- 6%, P =.63).
  
  
  CONCLUSIONS
  pH-stat cardiopulmonary bypass management improves neurologic outcome with deep hypothermic circulatory arrest compared with alpha-stat bypass. The mechanism of protection is not related to hemodynamics, hematocrit, glucose, or brain temperature.
authors.affiliation: Brain Research Laboratory, Department of Anesthesiology and Critical Care Medicine, The Children's Hospital of Philadelphia, 34th St. and Civic Center Blvd., Philadelphia, PA 19104, USA. Priestley@email.chop.edu
authors.forename: M A
authors.initials: MA
authors.lastname: Priestley
authors.forename: J A
authors.initials: JA
authors.lastname: Golden
authors.forename: I B
authors.initials: IB
authors.lastname: O'Hara
authors.forename: J
authors.initials: J
authors.lastname: McCann
authors.forename: C D
authors.initials: CD
authors.lastname: Kurth
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001925:Brain Damage, Chronic / Q000209:etiology* / Q000517:prevention & control; D002245:Carbon Dioxide / Q000008:administration & dosage* / Q000097:blood; D002315:Cardiopulmonary Bypass; D006324:Heart Arrest, Induced / Q000009:adverse effects / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D023421:Models, Animal; D009460:Neurologic Examination; D010100:Oxygen / Q000008:administration & dosage; D011597:Psychomotor Performance; D011897:Random Allocation; D013552:Swine; D016896:Treatment Outcome
chemical_list: D002245:Carbon Dioxide; D010100:Oxygen