title: Crystallization and preliminary X-ray crystallographic analysis of the TM1442 gene product from Thermotoga maritima, a homologue of Bacillus subtilis anti-anti-sigma factors.
abstract: A 110-residue protein encoded by the TM1442 gene of Thermotoga maritima shows amino-acid sequence similarity to Bacillus subtilis anti-anti-sigma factors RsbV and SpoIIAA. It has been overexpressed in Escherichia coli and the recombinant protein exists primarily as both a monomer and a dimer in solution. The dimeric form has been crystallized using polyethylene glycol (PEG) 8000 as a precipitant. Native X-ray diffraction data have been collected at 100 K to 2.0 A resolution. The crystals are monoclinic, belonging to the space group P2(1), with unit-cell parameters a = 31.54 (13), b = 116.83 (37), c = 31.39 (7) A, alpha = 90, beta = 119.84 (9), gamma = 90 degrees. The asymmetric unit contains two monomers of the recombinant polypeptide, with a corresponding V(M) of 2.24 A(3) Da(-1) and a solvent content of 45.0%.
authors.affiliation: School of Chemistry and Molecular Engineering, Seoul National University, Seoul 151-742, South Korea.
authors.forename: K S
authors.initials: KS
authors.lastname: Ha
authors.forename: J E
authors.initials: JE
authors.lastname: Kwak
authors.forename: B W
authors.initials: BW
authors.lastname: Han
authors.forename: J Y
authors.initials: JY
authors.lastname: Lee
authors.forename: J
authors.initials: J
authors.lastname: Moon
authors.forename: B I
authors.initials: BI
authors.lastname: Lee
authors.forename: S W
authors.initials: SW
authors.lastname: Suh
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D003460:Crystallization; D018360:Crystallography, X-Ray; D005798:Genes, Bacterial; D011994:Recombinant Proteins / Q000737:chemistry; D012808:Sigma Factor; D020124:Thermotoga maritima / Q000235:genetics*; D014157:Transcription Factors
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; C086522:RsbV protein, Bacteria; D012808:Sigma Factor; D014157:Transcription Factors; C092468:spoIIR protein, Bacillus subtilis; C030069:spore-specific proteins, Bacillus