Sodium iodide symporter (NIS) gene expression in human placenta.
The placenta must allow the passage of iodide from the maternal to the fetal circulation for synthesis of thyroxine by the fetal thyroid. The thyroid sodium iodide symporter (NIS) was cloned in 1996 and, although widely distributed among epithelial tissues, early studies failed to detect it in placenta. We demonstrated NIS mRNA in human placenta and in the human choriocarcinoma cell line, JAr. NIS protein was localized to trophoblasts, with a tendency to apical distribution, in sections of human placenta immunostained with a monoclonal antibody against hNIS. We conclude that NIS is expressed in placenta and may mediate placental iodide transport.
Mitchell
A M
AM
Royal Brisbane Hospital Research Foundation, Bancroft Centre, Clinical Research Centre, Brisbane, QLD 4029, Australia. agnieszM@qimr.edu.au
Manley
S W
SW
Morris
J C
JC
Powell
K A
KA
Bergert
E R
ER
Mortimer
R H
RH
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins / Q000032:analysis / Q000235:genetics*; D002822:Choriocarcinoma / Q000737:chemistry; D005260:Female; D015870:Gene Expression; D006111:Graves Disease / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics*; D010920:Placenta / Q000737:chemistry*; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D027981:Symporters; D013961:Thyroid Gland / Q000737:chemistry; D014018:Tissue Distribution; D014327:Trophoblasts / Q000737:chemistry; D014407:Tumor Cells, Cultured; D014594:Uterine Neoplasms / Q000737:chemistry; D000099209:Sodium-Iodide Symporters
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger; D027981:Symporters; D000099209:Sodium-Iodide Symporters