The use of large-scale cDNA analysis to profile differential gene expression in KYSE 410 human esophageal cancer cells after irradiation.
Atlas Human cDNA Expression Array, which includes 588 genes divided into functional classes, was used to study gene expression profiles in KYSE 410 human esophageal cancer cells irradiated in doses of 6 and 18 Gy. Considerable changes in the expression of a number of genes was found, including down-regulation of 3 cell cycle regulators and up-regulation of an apoptosis-related gene. Comparison of the expression profiles and identification of genes were performed with Atlas Vision 3.0 software.
Sebastian
J L
JL
Surgical Molecular Biology Research Laboratory, Wadsworth VAMC and Division of General Surgery, University of California, Los Angeles, California, USA. jsebasti@ucla.edu
Rigberg
D A
DA
Shrivatsan
E
E
Revasova
E
E
McFadden
D M
DM
Levingston
E H
EH
D004938:Esophageal Neoplasms / Q000235:genetics* / Q000532:radiotherapy; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects; D015894:Genome, Human; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D014407:Tumor Cells, Cultured