Extracellular polysaccharides do not inhibit the reaction between Streptococcus mutans and its specific immunoglobulin G (IgG) or penetration of the IgG through S. mutans biofilm.
The present study investigated whether extracellular polysaccharides inhibit reaction between Streptococcus mutans and its specific immunoglobulin G (IgG) and penetration of the IgG through S. mutans biofilm. The planktonic organisms with or without extracellular polysaccharides were prepared, incubated with rabbit IgG against whole cell of S. mutans and fluorescein isothiocyanate (FITC)-conjugated goat affinity purified antibody to rabbit IgG. Biofilms with or without extracellular polysaccharides were formed on cover glasses and incubated with rabbit IgG against S. mutans and FITC-conjugated goat antibody to rabbit IgG. Then, biofilms were stained with propidium iodide. The amount of specific IgG binding on S. mutans was determined by FITC intensity with a fluorescence microplate reader. The penetration of IgG through biofilms was determined by confocal laser scanning microscopy. The results showed that the fluorescence intensity of FITC in planktonic organisms with extracellular polysaccharides was similar to that in planktonic organisms without extracellular polysaccharides, indicating that extracellular polysaccharides did not inhibit the reaction between S. mutans and its specific IgG. Although biofilms of S. mutans with extracellular polysaccharides were much thicker and denser than those without extracellular polysaccharides, the speed with which IgG penetrated through both of the biofilms did not differ significantly, suggesting that penetration of IgG through S. mutans biofilm was not affected by extracellular polysaccharides.
Zhu
M
M
Cariology Research Unit, Department of Oral Microbiology, Niigata University School of Dentistry, Japan.
Takenaka
S
S
Sato
M
M
Hoshino
E
E
D000818:Animals; D018441:Biofilms; D004396:Coloring Agents; D016650:Fluorescein-5-isothiocyanate; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D006041:Goats; D007074:Immunoglobulin G / Q000276:immunology*; D018613:Microscopy, Confocal; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D011419:Propidium; D011817:Rabbits; D013223:Statistics as Topic; D013295:Streptococcus mutans / Q000276:immunology* / Q000502:physiology
D004396:Coloring Agents; D005456:Fluorescent Dyes; D007074:Immunoglobulin G; D011135:Polysaccharides, Bacterial; D011419:Propidium; D016650:Fluorescein-5-isothiocyanate