Detection of T suppressor cells in patients with organ allografts.
Specific immunosuppression of host's immune response to donor HLA antigens has been a major goal to clinical transplantation. Recent evidence has been accumulating to show that a distinct population of T cells expressing the CD8(+) CD28(-) phenotype display suppressor function and inhibit Th activation and proliferation by modulating the APC function. To assess the presence of Ts in transplant recipient's circulation, we have developed a flow cytometry method that measures the expression of costimulatory molecules on donor APC exposed to recipient Th and Ts. Our results demonstrate that quantitation of the capacity of CD8(+) CD28(-) T cells from patient circulation to suppress the activation of costimulatory molecules (CD80, CD86) on donor APC offers a reliable tool for monitoring specific immunosuppression against the graft in solid organ transplantation.
Ciubotariu
R
R
Department of Pathology, College of Physicians & Surgeons, Columbia University, New York, NY 10032, USA.
Vasilescu
R
R
Ho
E
E
Cinti
P
P
Cancedda
C
C
Poli
L
L
Late
M
M
Liu
Z
Z
Berloco
P
P
Cortesini
R
R
Suciu-Foca Cortesini
N
N
D000938:Antigen-Presenting Cells / Q000276:immunology / Q000378:metabolism; D019013:CD40 Antigens / Q000276:immunology / Q000378:metabolism; D002460:Cell Line; D018920:Coculture Techniques; D005434:Flow Cytometry; D016027:Heart Transplantation / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000032:analysis; D006801:Humans; D019169:Jurkat Cells; D016030:Kidney Transplantation / Q000276:immunology; D016031:Liver Transplantation / Q000276:immunology; D016377:Organ Transplantation; D016176:T-Lymphocyte Subsets / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D050378:T-Lymphocytes, Regulatory / Q000166:cytology* / Q000276:immunology; D014184:Transplantation, Homologous / Q000276:immunology; D014407:Tumor Cells, Cultured
D019013:CD40 Antigens; D015395:Histocompatibility Antigens Class I