title: More keys to padlock probes: mechanisms for high-throughput nucleic acid analysis.
abstract: With the impending availability of total information about nucleic acid sequences in humans and other organisms, tools to investigate these sequences on a large scale assume increasing importance. Methods currently in use, however, cannot offer the required combination of high-throughput, sensitivity and specificity of detection. Padlock probes, circularizing oligonucleotides, may provide a means to detect, distinguish, quantitate and also locate very large numbers of DNA or RNA sequences. Recent developments in areas such as the biochemistry of ligation and characterization of ligases, methods to replicate circularized probes and the development of assays based on these principles augment the potential of padlock probes.
authors.affiliation: The Beijer Laboratory, Department of Genetics and Pathology, Rudbeck Laboratory, Se-751 85, Uppsala, Sweden.
authors.forename: J
authors.initials: J
authors.lastname: Banér
authors.forename: M
authors.initials: M
authors.lastname: Nilsson
authors.forename: A
authors.initials: A
authors.lastname: Isaksson
authors.forename: M
authors.initials: M
authors.lastname: Mendel-Hartvig
authors.forename: D O
authors.initials: DO
authors.lastname: Antson
authors.forename: U
authors.initials: U
authors.lastname: Landegren
mesh_terms: D001709:Biotechnology / Q000379:methods; D004270:DNA, Circular; D015336:Molecular Probe Techniques; D009690:Nucleic Acid Conformation; D015345:Oligonucleotide Probes; D012313:RNA; D000079962:RNA, Circular
chemical_list: D004270:DNA, Circular; D015345:Oligonucleotide Probes; D000079962:RNA, Circular; D012313:RNA