Nerve growth factor regulates VR-1 mRNA levels in cultures of adult dorsal root ganglion neurons.
Nerve growth factor (NGF) regulates the nociceptive properties including sensitivity to capsaicin of a subset of dorsal root ganglion neurons, which express the high-affinity NGF receptor, trkA. Capsaicin sensitivity co-localizes with the expression of a cloned capsaicin receptor, vanilloid receptor type 1 (VR-1), which displays properties similar to the native capsaicin response. To determine whether VR-1 mRNA levels are regulated by NGF, VR-1 mRNA levels and the ability to respond to capsaicin by release of the neuropeptide calcitonin gene related peptide (CGRP) were measured as a function of NGF concentration in cultures of adult dorsal root ganglion neurons. NGF treatment increased both VR-1 mRNA expression and capsaicin evoked release of CGRP. These effects were inhibited by treatment with the trkA inhibitor k252a.
Winston
J
J
Department of Internal Medicine, Enteric Neuromuscular Diseases and Pain Laboratory, Division of Gastroenterology and Hepatology, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555, USA.
Toma
H
H
Shenoy
M
M
Pasricha
P J
PJ
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D002211:Capsaicin / Q000494:pharmacology; D002227:Carbazoles / Q000494:pharmacology; D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005727:Ganglia, Spinal / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D026121:Indole Alkaloids; D020932:Nerve Growth Factor / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020917:Receptor, trkA / Q000037:antagonists & inhibitors; D011955:Receptors, Drug / Q000096:biosynthesis*
D002227:Carbazoles; D004791:Enzyme Inhibitors; D026121:Indole Alkaloids; D012333:RNA, Messenger; D011955:Receptors, Drug; D020932:Nerve Growth Factor; C049985:staurosporine aglycone; D020917:Receptor, trkA; D015740:Calcitonin Gene-Related Peptide; D002211:Capsaicin