[Expression of interleukin-8 and its receptor in acute promyelocytic leukemia under all-trans retinoic acid treatment].
OBJECTIVE
To evaluate the clinical significance of expression of interleukin-(IL-8) and its type A receptor(IL-8RA) in acute promyelocytic leukemia(APL) patients under all trans-retinoic acid(ATRA) induction.


METHODS
Serum IL-8 level of 18 APL patients were dynamically studied(ELISA). Fresh APL cells from 3 patients were cultured with ATRA(10(-6) mmol/L). Supernatant IL-8 level and IL-8RA expression on APL cells were measured by FACS.


RESULTS
In vitro, IL-8 concentrations decreased 72 hours after incubation, while IL-8RA increased. In vivo, IL-8 increased more rapidly and markedly than temperature and WBC counts did before retinoic acid syndrome(RA-S) occurred. Serum IL-6 and IL-8 levels significantly increased when the patients suffered infection, and IL-8 increased even before fever. Both IL-8 and D-dimer increased while DIC progressed.


CONCLUSIONS
ATRA inhibited IL-8 secretion of APL cells while increased the expression of IL-8RA. Monitoring serum IL-8 concentrations could predict the development of RA-S and infection. Increase of both IL-8 and D-dimer concentrations suggested DIC progression.
Zeng
H
H
Department of Hematology, First Affiliated Hospital, Suzhou Medical College, Suzhou 215006.
Zhang
X
X
Tao
R
R
D000293:Adolescent; D000328:Adult; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis*; D015473:Leukemia, Promyelocytic, Acute / Q000188:drug therapy* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D023062:Receptors, Interleukin-8A / Q000096:biosynthesis; D014212:Tretinoin / Q000627:therapeutic use*
D000970:Antineoplastic Agents; D016209:Interleukin-8; D023062:Receptors, Interleukin-8A; D014212:Tretinoin