Different functional modulation by heterotropic ligands (2,3-diphosphoglycerate and chlorides) of the two haemoglobins from fallow-deer (Dama dama).
Two haemoglobin components have been identified and purified from fallow-deer (Dama dama) erythrocytes. They are present in similar amounts and the two tetrameric molecules share the same alpha chain, while two different beta chains are detected in the two components. The beta chains differ by 14 residues, even though they both have 145 amino-acid residues, which account for a molecular mass of 16,023 and 16,064 Da, respectively, while alpha chain has 141 residues, yielding a molecular mass of 15,142 Da. Compared with human Hb, the N-terminal region of both beta chains shows deletion of Val beta 1 and the replacement of His beta 2 by a methionyl residue, a modification which is common to most ruminant haemoglobins. Although both isolated components show a low intrinsic affinity for oxygen, meaningful differences between the two haemoglobins have been found with respect to the effect of heterotropic effectors, such as 2,3-diphosphoglycerate and chloride ions. In view of the high sequence homology between the two components, the different effect of heterotropic ligands has been tentatively correlated to possible localized structural variations between beta chains of the two haemoglobin components.
Angeletti
M
M
Department of Molecular, Cellular and Animal Biology, Post-Graduate School in Biochemistry and Clinical Chemistry, University of Camerino, Italy.
Pucciarelli
S
S
Priori
A M
AM
Canofeni
S
S
Barra
D
D
Fioretti
E
E
Coletta
M
M
D019794:2,3-Diphosphoglycerate / Q000494:pharmacology*; D000595:Amino Acid Sequence; D000818:Animals; D002712:Chlorides / Q000378:metabolism / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D003670:Deer; D004912:Erythrocytes / Q000378:metabolism*; D017353:Gene Deletion; D006454:Hemoglobins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D008024:Ligands; D008969:Molecular Sequence Data; D010100:Oxygen / Q000378:metabolism; D010710:Phosphates / Q000737:chemistry; D010802:Phylogeny; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D012984:Software; D013696:Temperature; D013997:Time Factors
D002712:Chlorides; D006454:Hemoglobins; D008024:Ligands; D010710:Phosphates; D019794:2,3-Diphosphoglycerate; D010100:Oxygen