title: Oleate, linoleate and cholesterol differently modify aspartyl- and glutamyl-aminopeptidase activities in primary cultures of rat astrocytes.
abstract: The intake of mono- and polyunsaturated fatty acids has been associated with a minor risk of cardiovascular diseases including hypertension. Changes in levels of fatty acids may also modify the cell activity and may be related with alterations in different regulatory processes. Aminopeptidases are zinc-metalloenzymes which metabolise circulating peptide hormones in several tissues. Glutamyl-aminopeptidase (GluAP) and to a lesser extent, aspartyl-aminopeptidase (AspAP), are related with angiotensin metabolism in the renin-angiotensin system. The present work was designed to study the effect of a range of concentrations (1-100 microM) of oleic and linoleic acids and cholesterol present in the culture medium on the activity of GluAP and AspAP in the culture of rat cerebral cortical astrocytes taken from 21-day-old fetuses. The results showed that oleic acid inhibits, while linoleic acid stimulates the activity of AspAP. Both fatty acids inhibit the activity of GluAP. Cholesterol stimulates the activity of both enzymes. On the basis of these results, a functional link may exit between the effects of fatty acids on hypertension and the modulation of aminopeptidase activity by these compounds in rat astrocytes, as an example of target cell type in the central nervous system.
authors.affiliation: Unit of Physiology, Faculty of Experimental and Health Sciences, University of Jaén, Paraje Las Lagunillas s/n, E-23071, Jaén, Spain. mramirez@ujaen.es
authors.forename: M J
authors.initials: MJ
authors.lastname: Ramírez-Expósito
authors.forename: J M
authors.initials: JM
authors.lastname: Martínez-Martos
authors.forename: M D
authors.initials: MD
authors.lastname: Mayas
authors.forename: M J
authors.initials: MJ
authors.lastname: García
authors.forename: I
authors.initials: I
authors.lastname: Prieto
authors.forename: G
authors.initials: G
authors.lastname: Aréchaga
authors.forename: M
authors.initials: M
authors.lastname: Ramírez
mesh_terms: D000626:Aminopeptidases / Q000378:metabolism*; D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000201:enzymology*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000201:enzymology; D002784:Cholesterol / Q000494:pharmacology*; D005333:Fetus; D043384:Glutamyl Aminopeptidase; D007700:Kinetics; D019787:Linoleic Acid / Q000494:pharmacology*; D019301:Oleic Acid / Q000494:pharmacology*; D051381:Rats
chemical_list: D019301:Oleic Acid; D002784:Cholesterol; D019787:Linoleic Acid; D000626:Aminopeptidases; D043384:Glutamyl Aminopeptidase