title: A new approach to the synthesis of APTRA indicators.
abstract: We have devised a general synthesis of Mg(2+) indicators which is based on the aminophenol triacetic acid (APTRA) structure. The key step is a palladium-catalyzed coupling reaction of a precursor of the APTRA ligand with a fluorescent group. This strategy resulted in new ratioable fluorescent APTRA indicators and the finding that the fluorescence response of these indicators is different for Mg(2+) and Ca(2+) in some cases. We believe that this represents a generally useful approach for combining fluorophore and chelator functionalities.
authors.affiliation: National Institute of Environmental Health Sciences, Laboratory of Structural Biology, P.O. Box 12233, MR-01, Research Triangle Park, North Carolina 27709, USA.
authors.forename: P A
authors.initials: PA
authors.lastname: Otten
authors.forename: R E
authors.initials: RE
authors.lastname: London
authors.forename: L A
authors.initials: LA
authors.lastname: Levy
mesh_terms: D000085:Acetates / Q000737:chemistry*; D002118:Calcium / Q000737:chemistry; D002384:Catalysis; D002614:Chelating Agents / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis; D007202:Indicators and Reagents; D007502:Iron Chelating Agents / Q000138:chemical synthesis; D008274:Magnesium / Q000737:chemistry*; D010165:Palladium / Q000737:chemistry; D010636:Phenols / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D000085:Acetates; D002614:Chelating Agents; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D007502:Iron Chelating Agents; D010636:Phenols; C424764:aminophenol triacetic acid; D010165:Palladium; D008274:Magnesium; D002118:Calcium