Effect of temperature on guinea pig urinary bladder contraction mediated via P2X-receptors.
In vitro experiments showed that P2X-receptor agonist alpha,beta-methylene-ATP and electrical field stimulation in the presence of muscarinic and alpha-adrenoreceptors blockers induced contractile responses of isolated guinea pig bladder, which were more pronounced at 30 degrees C than at 37 degrees C or 42 degrees C. P2X-receptor antagonist pyridoxal-6-phosphate-2',4'-disulfonic acid, produced a more potent inhibitory effect on contractions induced by electrical field stimulation at 30 degrees C in comparison with that at 37 degrees C or 42 degrees C, while the contractions induced by alpha,beta-methylene-ATP were similarly suppressed at all examined temperatures.
Ziganshin
A U
AU
Department of Pharmacology, Kazan State Medical University.
Rychkov
A V
AV
Ziganshina
L E
LE
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives; D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology; D000818:Animals; D001285:Atropine; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D006168:Guinea Pigs; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology*; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology; D010646:Phentolamine / Q000494:pharmacology; D058919:Purinergic P2 Receptor Antagonists; D011732:Pyridoxal Phosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D018048:Receptors, Purinergic P2 / Q000378:metabolism*; D058469:Receptors, Purinergic P2X; D013696:Temperature; D001743:Urinary Bladder / Q000502:physiology*
D000317:Adrenergic alpha-Antagonists; D058919:Purinergic P2 Receptor Antagonists; D018048:Receptors, Purinergic P2; D058469:Receptors, Purinergic P2X; C077792:pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid; D011732:Pyridoxal Phosphate; D001285:Atropine; D000255:Adenosine Triphosphate; C002630:alpha,beta-methyleneadenosine 5'-triphosphate; D010646:Phentolamine