title: Kendrin/pericentrin-B, a centrosome protein with homology to pericentrin that complexes with PCM-1.
abstract: The centrosome is responsible for nucleating microtubules and performing other cellular roles. To define the organization of the centrosome more completely, a human anti-centrosome serum was used to screen a human cDNA library, and a cDNA encoding a >350 kDa centrosome protein was identified. Sequence analyses revealed that this novel centrosome protein contains two coiled-coil domains bounded by non-coiled regions. The N-terminal region of the protein, named pericentrin-B, shares 61% identity (75% similarity) with pericentrin, suggesting an evolutionary relationship between these proteins. Antibodies against pericentrin-B stain centrosomes at all stages of the cell cycle, and pericentrin-B remains associated with centrosomes following microtubule depolymerization. Immunodepletion of neither pericentrin-B nor PCM-1 from cellular extracts inhibited the ability of salt-stripped centrosomes to recover microtubule nucleation potential, demonstrating that neither protein plays a key role in microtubule nucleation processes. Moreover, the binding of both PCM-1 and pericentrin-B with salt-stripped centrosomes required intact microtubules, demonstrating that the association of PCM-1 and pericentrin-B with centrosomes is a late event in the centrosome maturation process. Finally, pericentrin-B and PCM-1 coimmunoprecipitate, suggesting that PCM-1 and pericentrin-B form a functional complex in cells. This observation may help to explain the generation of anti-centrosome autoantibodies in certain autoimmune patients and may be important for centrosome function.
authors.affiliation: Department of Anatomy and Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, USA.
authors.forename: Q
authors.initials: Q
authors.lastname: Li
authors.forename: D
authors.initials: D
authors.lastname: Hansen
authors.forename: A
authors.initials: A
authors.lastname: Killilea
authors.forename: H C
authors.initials: HC
authors.lastname: Joshi
authors.forename: R E
authors.initials: RE
authors.lastname: Palazzo
authors.forename: R
authors.initials: R
authors.lastname: Balczon
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001324:Autoantigens / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D016466:CHO Cells; D002148:Calmodulin-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins; D018385:Centrosome / Q000378:metabolism*; D006224:Cricetinae; D017931:DNA Primers; D018076:DNA, Complementary; D005260:Female; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D011485:Protein Binding; D017386:Sequence Homology, Amino Acid
chemical_list: D000941:Antigens; D001324:Autoantigens; D002148:Calmodulin-Binding Proteins; D018797:Cell Cycle Proteins; D017931:DNA Primers; D018076:DNA, Complementary; C086074:PCM1 protein, human; C410004:kendrin; C086178:pericentrin