Contribution of mitochondria and peroxisomes to palmitate oxidation in pea tissues.
Total, mitochondrial and peroxisomal palmitate oxidation capacities were compared in pea, from the dry seed to 14 days after imbibition. Total beta-oxidation varied over the measured time period and showed four peaks of activity at day 2, days 5-6, day 10 and days 12-13. The contribution of peroxisomal and mitochondrial beta-oxidation to this overall beta-oxidation varied. Over the first 48 h of seed germination, peroxisomal beta-oxidation accounted for 80-100% of the total observed beta-oxidation. The larger peaks of beta-oxidation at days 5-6, day 10 and days 12-13 were due primarily to mitochondrial beta-oxidation activity, which accounted for 70-90% of the observed total beta-oxidation at these times. The peaks of activity are related to observed stages in seedling development.
Masterson
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Department of Biological and Nutritional Sciences, Newcastle University, Newcastle upon Tyne NE1 7RU, UK. christine.masterson@ncl.ac.uk
Wood
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C
D002245:Carbon Dioxide / Q000378:metabolism; D002250:Carbon Radioisotopes; D007700:Kinetics; D008928:Mitochondria / Q000378:metabolism*; D010084:Oxidation-Reduction; D019308:Palmitic Acid / Q000378:metabolism*; D018532:Pisum sativum / Q000254:growth & development / Q000378:metabolism*; D020675:Peroxisomes / Q000378:metabolism*; D013997:Time Factors
D002250:Carbon Radioisotopes; D002245:Carbon Dioxide; D019308:Palmitic Acid