title: Prolonged measles virus shedding in human immunodeficiency virus-infected children, detected by reverse transcriptase-polymerase chain reaction.
abstract: A reverse transcriptase-polymerase chain reaction assay was used to detect measles virus RNA in peripheral blood mononuclear cells, urine, and nasopharyngeal specimens from Zambian children during hospitalization and approximately 1-2 months after discharge. Of 47 children, 29 (61.7%) had prolonged measles virus shedding, as defined by detection of measles virus RNA in > or =1 specimen obtained 30-61 days after rash onset. Ten (90.9%) of 11 human immunodeficiency virus (HIV)-infected children had prolonged measles virus shedding, compared with 19 (52.8%) of 36 HIV-uninfected children (P=.02). Prolonged measles virus shedding did not correlate with levels of measles virus-specific antibody. HIV-infected children with measles may have a prolonged infectious period that potentially enhances measles virus transmission and hinders measles control.
authors.affiliation: W. Harry Feinstone Department of Molecular Microbiology and Immunology, School of Hygiene and Public Health, Johns Hopkins School of Public Health, Baltimore, MD 21205, USA.
authors.forename: S R
authors.initials: SR
authors.lastname: Permar
authors.forename: W J
authors.initials: WJ
authors.lastname: Moss
authors.forename: J J
authors.initials: JJ
authors.lastname: Ryon
authors.forename: M
authors.initials: M
authors.lastname: Monze
authors.forename: F
authors.initials: F
authors.lastname: Cutts
authors.forename: T C
authors.initials: TC
authors.lastname: Quinn
authors.forename: D E
authors.initials: DE
authors.lastname: Griffin
mesh_terms: D000293:Adolescent; D000914:Antibodies, Viral / Q000097:blood; D002648:Child; D002675:Child, Preschool; D005260:Female; D015658:HIV Infections / Q000150:complications*; D018023:HIV Seronegativity; D015497:HIV-1 / Q000302:isolation & purification; D006801:Humans; D007223:Infant; D007963:Leukocytes, Mononuclear / Q000821:virology; D008297:Male; D008457:Measles / Q000150:complications* / Q000821:virology; D008459:Measles virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification* / Q000502:physiology; D009305:Nasopharynx / Q000821:virology; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014556:Urine / Q000821:virology; D017201:Virus Shedding / Q000502:physiology; D015024:Zambia
chemical_list: D000914:Antibodies, Viral; D012367:RNA, Viral