title: Molecular analysis of Salmonella enteritidis isolates from the Caribbean by pulsed-field gel electrophoresis.
abstract: Using pulsed-field gel electrophoresis (PFGE), between 1987 and 1996 we analyzed Salmonella enteritidis isolates from gastroenteritis cases in four Caribbean countries: Barbados, Saint Kitts and Nevis, Saint Lucia, and Trinidad and Tobago. We also determined the resistance of the isolates to 12 antimicrobial agents. Of the 129 isolates of S. enteritidis available for testing, DNA digested by XbaI revealed 13 distinctive PFGE patterns. The most prevalent XbaI PFGE patterns were group 1 (88 of 129 isolates, 68.2%) and group 2 (26 of 129, 20.2%). The patterns found among S. enteritidis isolates correlated with the geographical origin of the isolates. Of the 28 isolates from Barbados, 20 of them (71.4%) belonged to XbaI PFGE group 2, and of the 93 isolates from Trinidad and Tobago, 78 of them (83.9%) belonged to group 1. SpeI digestion of S. enteritidis genome was not as discriminatory as XbaI. Overall, of the 129 isolates, 67 of them (51.9%) exhibited resistance to one or more of the 12 antimicrobial agents that we tested. The prevalence of resistance was 53.8% for the S. enteritidis isolates tested from Trinidad and Tobago, 50.0% for those from Barbados, 28.6% for those from Saint Lucia, and 100.0% for one isolate from the island of Saint Kitts. Resistance was highest to triple sulfur (59 of 129 isolates, 45.7%), followed by furadantoin (10 of 129, 7.8%), ampicillin (7 of 129, 5.4%), and carbamycin (5 of 129, 3.9%).
authors.affiliation: University of the West Indies, Faculty of Medical Sciences, School of Veterinary Medicine, St. Augustine, Trinidad and Tobago. abiodunadesiyun@hotmail.com
authors.forename: A
authors.initials: A
authors.lastname: Adesiyun
authors.forename: A
authors.initials: A
authors.lastname: Carson
authors.forename: K
authors.initials: K
authors.lastname: McAdoo
authors.forename: C
authors.initials: C
authors.lastname: Bailey
mesh_terms: D001460:Barbados; D004269:DNA, Bacterial / Q000032:analysis*; D004352:Drug Resistance, Microbial; D016521:Electrophoresis, Gel, Pulsed-Field; D019437:Saint Kitts and Nevis; D019431:Saint Lucia; D012477:Salmonella enteritidis / Q000145:classification / Q000187:drug effects / Q000235:genetics*; D014300:Trinidad and Tobago
chemical_list: D004269:DNA, Bacterial