MR imaging in rat glioma model and gene therapy using EGFR antisence RNA.
OBJECTIVE
To study the value of 1.5T MRI in long-term follow-up of rat C6 glioma model and the efficacy of EGFR antisence RNA therapy in vivo.


METHODS
Forty-three male Wistar rats, weighing 150 to 200 g, were used for this study. They were divided into four groups: group I (7 normal rats); group II (16 rats inoculated C6 cells in the right caudate nucleus); group III (12 rats with the C6 gliomas treated with EGFR antisence RNA by in site injection); and group IV (8 rats inoculated C6 cells transfected with EGFR antisence RNA in the right caudate nucleus). Group I was examined by plain and enhanced MR scanning. Group II-IV were followed up by plain and enhanced MR scanning and were sacrificed in variable time points for pathological examination.


RESULTS
Cerebral hemispheres of normal rat were shown clearly on the MR image. The tumor could be seen about 1 week after inoculation. According to the findings on the follow-up MR scan, we could observe growth of the tumor or its regression after treatment. The tumor growth was significantly inhibited in group III and group IV as compared to those in group II.


CONCLUSIONS
The growth of the rat C6 glioma model and its change after treatment could be shown clearly in 1.5T MR imaging; EGFR antisence RNA significantly inhibited the growth of glioma in vivo.
Zhang
Y
Y
Department of Radiology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
Cui
J
J
Liu
X
X
Pu
P
P
Wang
G
G
Wu
E
E
D000818:Animals; D001254:Astrocytoma / Q000175:diagnosis / Q000628:therapy; D001932:Brain Neoplasms / Q000175:diagnosis / Q000628:therapy*; D018773:Genes, erbB-1 / Q000235:genetics*; D015316:Genetic Therapy; D005910:Glioma / Q000175:diagnosis / Q000628:therapy*; D008297:Male; D009368:Neoplasm Transplantation; D016372:RNA, Antisense / Q000235:genetics / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D014162:Transfection; D014407:Tumor Cells, Cultured
D016372:RNA, Antisense