Metallothionein isogene transcription in red blood cell precursors from human cord blood.
The in vitro transcription patterns for 10 functional metallothionein (MT) isogenes have been investigated in red blood cell (RBC) precursors from human cord blood. Active transcription status of the isogenes, MT-0, MT-1A, MT-1B, MT-1E, MT-1G, MT-1X, and MT-2A, was detected in both ex vivo expanded RBC precursors (burst-forming unit-erythroid) and glycophorin A(+) and CD71(+) cells separated by magnetic cell sorting. Transcription patterns of these isogenes were analyzed at different times of incubation with the addition of Zn supplement. In neither the ex vivo expanded precursors nor glycophorin A(+) and CD71(+) cells could MT-1F and MT-3 be detected. Transcripts of MT-4 were detected in glycophorin A(+) and CD71(+) cells. Erythropoietin-responsive constitutive transcription of MT-1X and possible interleukin-3-responsive downregulation of MT-2A in ex vivo expanded precursors reveal their effect on MT biosynthesis. Biosynthesis and induction of MT at the protein level in the RBC precursors was also demonstrated by immunoblotting.
Rahman
M T
MT
Laboratory for Biochemistry, Department of Chemistry, Katholieke Universiteit Leuven, Belgium.
De Ley
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D015703:Antigens, CD / Q000378:metabolism; D000944:Antigens, Differentiation, B-Lymphocyte / Q000378:metabolism; D002469:Cell Separation; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000378:metabolism; D015536:Down-Regulation; D004587:Electrophoresis, Agar Gel; D004912:Erythrocytes / Q000378:metabolism*; D004921:Erythropoietin / Q000378:metabolism; D005312:Fetal Blood / Q000378:metabolism*; D006021:Glycophorins / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D007377:Interleukin-3 / Q000378:metabolism; D008668:Metallothionein / Q000235:genetics* / Q000378:metabolism*; D011061:Poly A / Q000378:metabolism; D020033:Protein Isoforms; D012333:RNA, Messenger / Q000378:metabolism; D011990:Receptors, Transferrin; D014158:Transcription, Genetic; D015032:Zinc / Q000378:metabolism / Q000494:pharmacology
D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; C068126:CD71 antigen; D018076:DNA, Complementary; D006021:Glycophorins; D007377:Interleukin-3; D020033:Protein Isoforms; D012333:RNA, Messenger; D011990:Receptors, Transferrin; D004921:Erythropoietin; D011061:Poly A; D008668:Metallothionein; D015032:Zinc