Monitoring of immune response by blood serum profiling using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
The application of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) to the analysis of blood serum leads to the observation of a large variety of reproducible mass spectral peaks corresponding to blood components. In this study, the use of MALDI-TOFMS was developed as a tool for monitoring immune response to bacterial infection. Employing the MALDI-TOFMS approach, the levels of many components of blood were found to be immune response independent whereas others were found to correlate directly with the response of the immune system to two known types of bacteria (Staphylococcus aureus and Enterococcus faecalis). The methodologies reported here should be useful for the rapid monitoring of blood, especially that of the immune response mechanisms in various animal species.
Haag
A M
AM
Department of Chemistry, University of New Orleans, Lakefront, New Orleans, Louisiana 70148, USA.
Chaiban
J
J
Johnston
K H
KH
Cole
R B
RB
D000818:Animals; D001424:Bacterial Infections / Q000276:immunology* / Q000382:microbiology; D001798:Blood Proteins / Q000032:analysis*; D013293:Enterococcus faecalis; D016908:Gram-Positive Bacterial Infections / Q000276:immunology; D051379:Mice; D008819:Mice, Nude; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013203:Staphylococcal Infections / Q000276:immunology
D001798:Blood Proteins