High production of D-tagatose, a potential sugar substitute, using immobilized L-arabinose isomerase.
An L-arabinose isomerase of Escherichia coli was immobilized using covalent binding to agarose to produce D-tagatose, a bulking sweetener that can be economically used as a sugar substitute. The immobilized L-arabinose isomerase stably produced an average of 7.5 g-tagatose/L.day for 7 days with a productivity exceeding that of the free enzyme (0.47 vs 0.30 mg/U.day). Using a scaled-up immobilized enzyme system, 99.9 g-tagatose/L was produced from galactose with 20% equilibrium in 48 h. The process was repeated two more times with production of 104.1 and 103.5 g-tagatose/L. D-Tagatose production using an immobilized L-arabinose isomerase has a high potential for commercial application.
Kim
P
P
R&D Center, Tong Yang Confectionery Co., Seoul 140-715, Korea. pil@tyc.co.kr
Yoon
S H
SH
Roh
H J
HJ
Choi
J H
JH
D019747:Aldose-Ketose Isomerases / Q000378:metabolism*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D006601:Hexoses / Q000096:biosynthesis*; D012996:Solutions
D004800:Enzymes, Immobilized; D006601:Hexoses; D012996:Solutions; D019747:Aldose-Ketose Isomerases; C019715:L-arabinose isomerase; C030192:tagatose