Synthesis, analysis, purification, and intracellular delivery of peptide nucleic acids.
Peptide nucleic acids (PNAs) are nonionic DNA mimics. Their novel chemical properties may facilitate the development of selective and potent antisense and antigene strategies for regulating intracellular processes. Described herein are procedures for the synthesis, purification, handling, and characterization of PNAs. A simple protocol for the lipid-mediated introduction of PNAs into in vitro cultures of mammalian cells is provided.
Braasch
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Department of Pharmacology, Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas 75390-9041, USA.
Corey
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D000818:Animals; D019556:COS Cells; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D005821:Genetic Techniques; D008156:Luciferases / Q000378:metabolism; D008956:Models, Chemical; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis* / Q000302:isolation & purification* / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013696:Temperature; D014162:Transfection
D020135:Peptide Nucleic Acids; D008156:Luciferases