Cellular response and membrane formation on the surface of implanted posterior chamber intraocular lens in rabbit.
OBJECTIVE
In order to study the cellular response and membrane formation on the surface of intraocular lens after cataract surgery and lens implantation, this study was designed to observe the deposits on the surface of implanted posterior chamber intraocular lens (PC-IOL) for 4, 7, 15, 30 and 90 days in rabbits.


METHODS
30 rabbits had undergone ECCE with PC-IOL implantation. PC-IOLs were removed at different stages after operation. 20 PC-IOLs were stained by HE to demonstrate cells on the surface of PC-IOL under light microscopy. 5 PC-IOLs for scanning electron microscopy were observed. 5 membranes were studied by transmission electron microscopy.


RESULTS
On 25 implanted PC-IOLs, the incidence of cell adhesion was 100%. The cells adherent to lens included macrophages, fibroblast-like cells, epithelioid cells, giant cells, ultralarge giant cells and lymphocytes. It was seen that there was a thin proteinaceous film on the surface of the implanted PC-IOLs and the membrane was composed of fibrin collagen fibrils, macrophages, fibroblast-like cells, giant cells and fibroblasts.


CONCLUSIONS
The cellular response on the surface of implanted PC-IOL is a chronic foreign-body inflammatory reaction and the membrane on the surface of implanted PC-IOL is a reactive membrane of the foreign-body. However, the reaction described in this study might be applicable to human since cellular elements and membranes have been frequently reported finding in human.
Yang
F
F
Department of Ophthalmology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Li
S
S
Liu
Y
Y
D000818:Animals; D002448:Cell Adhesion; D005260:Female; D005347:Fibroblasts / Q000502:physiology / Q000648:ultrastructure; D005547:Foreign Bodies / Q000473:pathology*; D015726:Giant Cells / Q000502:physiology / Q000648:ultrastructure; D019654:Lens Implantation, Intraocular / Q000009:adverse effects* / Q000379:methods; D007910:Lenses, Intraocular; D008264:Macrophages / Q000502:physiology* / Q000648:ultrastructure; D008297:Male; D011817:Rabbits