Effects of antiprogestins RU486 and ZK98299 on the expression of cell cycle proteins of a medroxyprogesterone acetate (MPA)-induced murine mammary tumor.
This study links the tumor inhibitory effect of anti-progestins RU486 and ZK98299 to the expression of cell cycle proteins. Medroxyprogesterone acetate-induced ductal mammary adenocarcinoma-bearing female BALB/c mice were treated daily either with RU486 or ZK98299, observing tumor growth retardation. p21 increased after 24 h treatment, peaked at day 4 and returned to control levels at day 7. Cyclin D1, cyclin E, CDK2 and CDK4, did not change during treatment. These results suggest that p21 might play a role in early inhibitory stages of tumor growth induced by RU486 and ZK98299.
Peters
M G
MG
Instituto de Biología y Medicina Experimental, CONICET, Buenos Aires, Argentina. mgpeters@hotmail.com
Vanzulli
S
S
Elizalde
P V
PV
Charreau
E H
EH
Goin
M M
MM
D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use; D042846:CDC2-CDC28 Kinases; D018797:Cell Cycle Proteins / Q000032:analysis*; D002455:Cell Division / Q000187:drug effects; D019938:Cyclin D1 / Q000032:analysis; D019927:Cyclin E / Q000032:analysis; D051357:Cyclin-Dependent Kinase 2; D051358:Cyclin-Dependent Kinase 4; D050759:Cyclin-Dependent Kinase Inhibitor p21; D018844:Cyclin-Dependent Kinases / Q000032:analysis; D016213:Cyclins / Q000032:analysis; D004791:Enzyme Inhibitors / Q000032:analysis; D005260:Female; D006067:Gonanes / Q000627:therapeutic use*; D006727:Hormone Antagonists / Q000627:therapeutic use*; D008325:Mammary Neoplasms, Experimental / Q000188:drug therapy* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D015735:Mifepristone / Q000627:therapeutic use*; D017346:Protein Serine-Threonine Kinases / Q000032:analysis; D011518:Proto-Oncogene Proteins; D013997:Time Factors
D000970:Antineoplastic Agents; C493957:Cdkn1a protein, mouse; D018797:Cell Cycle Proteins; D019927:Cyclin E; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins; D004791:Enzyme Inhibitors; D006067:Gonanes; D006727:Hormone Antagonists; D011518:Proto-Oncogene Proteins; D019938:Cyclin D1; D015735:Mifepristone; D017346:Protein Serine-Threonine Kinases; D042846:CDC2-CDC28 Kinases; C495893:Cdk2 protein, mouse; C495902:Cdk4 protein, mouse; D051357:Cyclin-Dependent Kinase 2; D051358:Cyclin-Dependent Kinase 4; D018844:Cyclin-Dependent Kinases; C053238:onapristone