Effect of tetra-peptide isolated from interleukin 1 (IL-1) on corneal epithelial wound healing in the rabbit.
To develop a new method for wound healing in case of injured corneal epithelium, the effects of the tetrapeptide (Val-Leu-Leu-Lys), showing the consensus sequence between human interleukin (IL)-1alpha and bovine parotid protein (parotin) on epithelial cell proliferation and elongation were analysed in vitro cell culture experiments on epithelial cells obtained from rabbit cornea. The peptide showed dose-dependent stimulatory effects on epithelial cell proliferation and elongation at 10-100 microg ml(-1)compared with the control experiments. Furthermore, the peptide also exhibited a significant wound healing activity for the epithelial cells in an in situ experiment using mechanically injured rabbit cornea, while the higher concentration of the peptide (100 microg ml(-1)) showed greater efficient results than a previously known agent, sodium hyaluronate (0.3%). In addition, no pyrogenic activity of this peptide was detected by the previously established pyrogenicity test using rabbits. These results suggest that the tetrapeptide (Val-Leu-Leu-Lys) is a promising agent for wound healing in the case of injured corneal epithelium.
Hara
Y
Y
Department of Ophthalmology, Nara Medical University, Nara, Kashihara-Shi, 630-8522, Japan.
Matsuura
T
T
Tsukamoto
M
M
Ishizaka
S
S
Saishin
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001831:Body Temperature / Q000187:drug effects; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D019573:Epithelium, Corneal / Q000166:cytology / Q000187:drug effects / Q000293:injuries*; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics* / Q000627:therapeutic use*; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000627:therapeutic use; D011817:Rabbits; D013268:Stimulation, Chemical; D014945:Wound Healing
D007375:Interleukin-1; D010446:Peptide Fragments