TRP-PLIK, a bifunctional protein with kinase and ion channel activities.
We cloned and characterized a protein kinase and ion channel, TRP-PLIK. As part of the long transient receptor potential channel subfamily implicated in control of cell division, it is a protein that is both an ion channel and a protein kinase. TRP-PLIK phosphorylated itself, displayed a wide tissue distribution, and, when expressed in CHO-K1 cells, constituted a nonselective, calcium-permeant, 105-picosiemen, steeply outwardly rectifying conductance. The zinc finger containing alpha-kinase domain was functional. Inactivation of the kinase activity by site-directed mutagenesis and the channel's dependence on intracellular adenosine triphosphate (ATP) demonstrated that the channel's kinase activity is essential for channel function.
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Howard Hughes Medical Institute, Department of Cardiology, Department of Neurobiology, Harvard Medical School, 1309 Enders Building, 320 Longwood Avenue, Children's Hospital, Boston, MA 02115, USA.
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Clapham
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D000255:Adenosine Triphosphate / Q000378:metabolism; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells; D002118:Calcium / Q000378:metabolism; D020134:Catalytic Domain; D002412:Cations / Q000378:metabolism; D002460:Cell Line; D006224:Cricetinae; D018076:DNA, Complementary; D004553:Electric Conductivity; D006801:Humans; D007473:Ion Channels / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D008565:Membrane Proteins; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D004676:Myelin Basic Protein / Q000378:metabolism; D018408:Patch-Clamp Techniques; D010766:Phosphorylation; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D050053:TRPM Cation Channels; D014162:Transfection; D020798:Two-Hybrid System Techniques; D010738:Type C Phospholipases / Q000378:metabolism
D002412:Cations; D018076:DNA, Complementary; D007473:Ion Channels; D008565:Membrane Proteins; D004676:Myelin Basic Protein; D011993:Recombinant Fusion Proteins; D050053:TRPM Cation Channels; D000255:Adenosine Triphosphate; D011494:Protein Kinases; C421903:Trpm7 protein, mouse; D017346:Protein Serine-Threonine Kinases; C486583:TRPM7 protein, human; C486584:Trpm7 protein, rat; D010738:Type C Phospholipases; D002118:Calcium