A simple and easy method for the monitoring of environmental pollutants using oligotrophic bacteria.
Sphingomonas paucimobilis KPS01, an oligotrophic bacterium isolated from soil, may be a useful tool for monitoring heavy metals. Previous methods relying on counting of viable cells require a relatively long time and some skill; we have developed a method based on optical density (O.D.) measurements which is significantly faster and does not require skilled personnel. The results of the O.D. and viable count methods were consistent; both methods detected heavy metals at concentrations ranging from 10-3 to 10-5 mmol l-1 and identified heavy metal contamination in 13 of 18 river water samples. Our results demonstrate that biological detection using this O.D. method and S. paucimobilis KPS01 may be useful for routine environmental monitoring of heavy metals, particularly in water sources.
Tada
Y
Y
Department of Bioengineering, Faculty of Biology Oriented Science & Technology, Kinki University, Wakayama, Japan. tada@bio.waka.kindai.ac.jp
Kobata
T
T
Nakaoka
C
C
D015169:Colony Count, Microbial; D004784:Environmental Monitoring / Q000379:methods; D005618:Fresh Water; D019216:Metals, Heavy / Q000032:analysis* / Q000494:pharmacology*; D013054:Spectrophotometry, Atomic / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D020578:Sphingomonas / Q000187:drug effects* / Q000254:growth & development*; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000494:pharmacology
D019216:Metals, Heavy; D014874:Water Pollutants, Chemical