Genomic instability in Down syndrome and Fanconi anemia assessed by micronucleus analysis and single-cell gel electrophoresis.
Cytokinesis-block micronucleus (CB-MN) assay and single-cell gel electrophoresis (SCGE) were employed to analyze leukocytes from 14 Fanconi anemia (FA) patients, 30 Down syndrome (DS) patients, and 30 control individuals, to examine the sensitivity of these techniques to detect genomic instability in these 2 diseases. The DS patients presented increased DNA damage as measured by SCGE in relation to controls. The frequencies of micronuclei and dicentric bridges were similar to those of controls. Micronucleus frequency, dicentric bridge frequencies, and DNA damage were higher in FA patients than in controls. The high frequency of micronuclei observed in FA patients seems to be due to clastogenic events, because an increase in the frequency of dicentric bridges was also observed. Micronuclei are expressed mutations and need cell division to appear. The damage detected by SCGE is repairable, and does not require cell division. Under alkaline conditions, SCGE assesses double- and single-strand breaks and alkali-labile sites. The 2 methods are efficient for monitoring mutagenic events in exposed populations or in individuals with genetic instability. While the damage measured by micronucleus analysis is accumulated over a long period of time, DNA damage measured by SCGE reflects recent, unrepaired events.
Maluf
S W
SW
Medical Genetics Service, Hospital de Clínicas, Rua Ramiro Barcelos, 2350, 90035-003, Porto Alegre, Brazil. sharbel@vol.com.br
Erdtmann
B
B
D000293:Adolescent; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D020552:Comet Assay; D004314:Down Syndrome / Q000235:genetics*; D005199:Fanconi Anemia / Q000235:genetics*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D015162:Micronucleus Tests; D012680:Sensitivity and Specificity