A calmodulin binding protein from Arabidopsis is induced by ethylene and contains a DNA-binding motif.
Calmodulin (CaM), a key calcium sensor in all eukaryotes, regulates diverse cellular processes by interacting with other proteins. To isolate CaM binding proteins involved in ethylene signal transduction, we screened an expression library prepared from ethylene-treated Arabidopsis seedlings with 35S-labeled CaM. A cDNA clone, EICBP (Ethylene-Induced CaM Binding Protein), encoding a protein that interacts with activated CaM was isolated in this screening. The CaM binding domain in EICBP was mapped to the C-terminus of the protein. These results indicate that calcium, through CaM, could regulate the activity of EICBP. The EICBP is expressed in different tissues and its expression in seedlings is induced by ethylene. The EICBP contains, in addition to a CaM binding domain, several features that are typical of transcription factors. These include a DNA-binding domain at the N terminus, an acidic region at the C terminus, and nuclear localization signals. In database searches a partial cDNA (CG-1) encoding a DNA-binding motif from parsley and an ethylene up-regulated partial cDNA from tomato (ER66) showed significant similarity to EICBP. In addition, five hypothetical proteins in the Arabidopsis genome also showed a very high sequence similarity with EICBP, indicating that there are several EICBP-related proteins in Arabidopsis. The structural features of EICBP are conserved in all EICBP-related proteins in Arabidopsis, suggesting that they may constitute a new family of DNA binding proteins and are likely to be involved in modulating gene expression in the presence of ethylene.
Reddy
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AS
Department of Biology and Program in Cell and Molecular Biology, Colorado State University, Fort Collins, Colorado 80523, USA. reddy@lamar.colostate.edu
Reddy
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VS
Golovkin
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M
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects / Q000378:metabolism*; D002148:Calmodulin-Binding Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D018076:DNA, Complementary / Q000032:analysis; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D005030:Ethylenes / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010944:Plants / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D002148:Calmodulin-Binding Proteins; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D005030:Ethylenes; D010937:Plant Growth Regulators; C036216:ethylene