Photochemistry. Twist and fluoresce.
Catalytic antibodies, or artificial enzymes, act as catalysts by lowering the energy of the transition state of a reaction. In his Perspective, Brauman highlights the study by Simeonov et al., who have applied similar principles to photochemical processes and shown that intense fluorescence can be achieved. The approach provides a way of measuring internal molecular motion within the antibody on a very fast time scale. Fluorescent antibodies may find applications in immunochemistry, histological assays, and genomic studies.
Brauman
J I
JI
Department of Chemistry, Stanford University, Stanford, CA 94305-5080, USA. brauman@stanford.edu
D017151:Antibodies, Catalytic / Q000737:chemistry*; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D000936:Antigen-Antibody Complex / Q000737:chemistry; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D005453:Fluorescence; D006241:Haptens; D010777:Photochemistry; D017785:Photons; D013237:Stereoisomerism; D013267:Stilbenes / Q000737:chemistry* / Q000276:immunology*; D013696:Temperature
D017151:Antibodies, Catalytic; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D006241:Haptens; D013267:Stilbenes