Morphological and metabolic changes in transgenic wheat with altered glycerol-3-phosphate acyltransferase or acyl-acyl carrier protein (ACP) thioesterase activities.
We have transformed varieties of wheat with a Pisum sativum glycerol-3-phosphate acyltransferase gene, and also with an Arabidopsis thaliana acyl-ACP thioesterase gene. Morphological (growth, organelle development) and metabolic changes (fatty acid labelling of chloroplast and non-chloroplast lipids) have been observed in transgenics with altered gene expression for either enzyme.
Edlin
D A
DA
School of Biosciences, Cardiff University, Cardiff CF10 3US, UK.
Kille
P
P
Wilkinson
M D
MD
Jones
H D
HD
Harwood
J L
JL
D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D002736:Chloroplasts / Q000502:physiology; D005227:Fatty Acids / Q000096:biosynthesis*; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D005992:Glycerol-3-Phosphate O-Acyltransferase / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D008928:Mitochondria / Q000502:physiology; D018532:Pisum sativum / Q000201:enzymology* / Q000235:genetics; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D013869:Thiolester Hydrolases / Q000235:genetics / Q000378:metabolism*; D014908:Triticum / Q000201:enzymology / Q000502:physiology*
D005227:Fatty Acids; D005992:Glycerol-3-Phosphate O-Acyltransferase; D013869:Thiolester Hydrolases; C018748:oleoyl-(acyl-carrier-protein) hydrolase