title: Gcn4 activator targets Gcn5 histone acetyltransferase to specific promoters independently of transcription.
abstract: Histone acetylation correlates well with transcriptional activity, and histone acetyltransferases (HATs) selectively regulate subsets of target genes by mechanisms that remain unclear. Here, we provide in vivo evidence that the yeast transcriptional activator Gcn4 recruits Gcn5 HAT complexes to selective promoters positioned in natural or ectopic locations, thereby creating local domains of histone H3 hyperacetylation and subsequent transcriptional activation. A significant portion of the Gcn4-targeted histone acetylation by Gcn5 is independent of transcriptional activity. These observations provide strong evidence for promoter-selective, targeted histone acetylation by Gcn5 that facilitates transcription in a causal fashion. In addition, Gcn5 also functions in an untargeted manner to acetylate H3 on a genome-wide scale.
authors.affiliation: Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824, USA.
authors.forename: M H
authors.initials: MH
authors.lastname: Kuo
authors.forename: E
authors.initials: E
authors.lastname: vom Baur
authors.forename: K
authors.initials: K
authors.lastname: Struhl
authors.forename: C D
authors.initials: CD
authors.lastname: Allis
mesh_terms: D000107:Acetylation; D000123:Acetyltransferases / Q000378:metabolism*; D002843:Chromatin / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal / Q000235:genetics; D016681:Genome, Fungal; D051548:Histone Acetyltransferases; D006657:Histones / Q000378:metabolism; D011233:Precipitin Tests; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011494:Protein Kinases / Q000737:chemistry / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D012331:RNA, Fungal / Q000032:analysis / Q000235:genetics; D020218:Response Elements / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D013379:Substrate Specificity; D015534:Trans-Activators / Q000378:metabolism*; D014158:Transcription, Genetic / Q000235:genetics; D015533:Transcriptional Activation; D015003:Yeasts / Q000201:enzymology / Q000235:genetics*; D000099224:p300-CBP-Associated Factor
chemical_list: D000123:Acetyltransferases; D002843:Chromatin; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D051548:Histone Acetyltransferases; D006657:Histones; D011494:Protein Kinases; D012331:RNA, Fungal; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; C501392:GCN5 protein, S cerevisiae; D000099224:p300-CBP-Associated Factor