title: Successful reinfection of chronically infected mice by a different Toxoplasma gondii genotype.
abstract: It is generally assumed that primary infection by Toxoplasma gondii protects from reinfection. A recent study using a murine model has questioned this dogma using indirect procedures to detect the reinfecting strain. We have reinvestigated this issue using a transfected strain of T. gondii (Prugniaud beta galactosidase: Pru beta gal) which expresses Escherichia coli beta-galactosidase. Detection of enzyme activity on fixed parasites allows a direct distinction between transfected and untransfected strains. We have found that in OF1 mice primary infection with the 76 K strain of T. gondii fully protects mice against tissue cyst production upon reinfection with the Pru beta gal T. gondii strain whereas primary infection with the Pru beta gal T. gondii strain does not impair tissue cyst formation upon reinfection with the Ned strain of T. gondii, which belongs to another T. gondii genotype. These results suggest that the immune protection conferred by one strain of T. gondii can be breached by reinfection with a strain belonging to another genotype; which can have significant consequences in human or veterinary medicine.
authors.affiliation: Service de Parasitologie-Mycologie, CHU Brabois-Nancy, Allée du Morvan, 54511 Vandoeuvre les Nancy., Cedex, France. anne.dao@medecine.u-nancy.fr
authors.forename: A
authors.initials: A
authors.lastname: Dao
authors.forename: B
authors.initials: B
authors.lastname: Fortier
authors.forename: M
authors.initials: M
authors.lastname: Soete
authors.forename: F
authors.initials: F
authors.lastname: Plenat
authors.forename: J F
authors.initials: JF
authors.lastname: Dubremetz
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000201:enzymology; D005838:Genotype; D008297:Male; D051379:Mice; D012008:Recurrence; D012376:Rodent Diseases / Q000276:immunology*; D013045:Species Specificity; D014122:Toxoplasma / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity*; D014124:Toxoplasmosis, Animal / Q000276:immunology*; D014162:Transfection; D001616:beta-Galactosidase / Q000096:biosynthesis
chemical_list: D001616:beta-Galactosidase