[DNA sequencing of variable regions of anti-idiotypic monoclonal antibody SM6 to B-CLL].
OBJECTIVE
To analyze the variable region sequences of an anti-idiotypic monoclonal antibody to human chronic B cell leukemia (B-CLL).


METHODS
The variable region sequences of light and heavy chains (VL, VH) of anti-idiotypic McAb SM6 to B-CLL were analyzed by their cDNA cloning and sequencing. Total RNA was extracted from a hybridoma cell line producing SM6. The VH and VL genes were amplified by RT-PCR with specific primers. The PCR products were cloned into pGEM-T vectors, then transfected into JM109.


CONCLUSIONS
It was confirmed by DNASIS7 sequence analysis that the full-length and potentially functional genes were successfully cloned. The VH gene expressed in SM6 belongs to Q52, the JH region using the JH3. The VL gene belongs to VK19, the JK region using JK2.
Su
N
N
Department of Immunology, Tongji Medical University, Wuhan 430030.
Yang
J
J
Wang
X
X
D000888:Antibodies, Anti-Idiotypic / Q000235:genetics*; D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm / Q000235:genetics*; D001483:Base Sequence; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D007135:Immunoglobulin Variable Region