A patatin-like protein with galactolipase activity is induced by drought stress in Vigna unguiculata leaves.
This paper reports the cloning of a cDNA (Vupat1) expressed in Vigna unguiculata leaves coding for a protein with 48% sequence homology to patatin, the major protein from potato tuber which has lipolytic acylhydrolase activity. Two cultivars differing in drought tolerance were examined in Northern-blot analyses. Expression of Vupat1 is stimulated by drought stress, especially in the drought-sensitive cultivar. Vupat1 was expressed in the baculovirus system as a fusion protein secreted in the culture medium. The recombinant protein displays lipolytic activity towards monogalactosyldiacylglycerol, digalactosyldiacylglycerol and sulphoquinovosyldiacylglycerols.
Matos
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AR
Laboratoire de Biochimie et Physiologie de l'Adaptation Végétale, Université Paris 7 Denis Diderot, case 7019, 2 Place Jussieu, 75251 Paris Cedex 05, France. matos@jussieu.fr
d'Arcy-Lameta
A
A
França
M
M
Zuily-Fodil
Y
Y
Pham-Thi
A T
AT
D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000096:biosynthesis / Q000378:metabolism*; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004075:Diglycerides / Q000378:metabolism; D004190:Disasters; D004790:Enzyme Induction; D007887:Fabaceae / Q000201:enzymology / Q000502:physiology*; D008066:Lipolysis; D018515:Plant Leaves / Q000201:enzymology; D010940:Plant Proteins / Q000096:biosynthesis / Q000378:metabolism*; D010946:Plants, Medicinal; D011994:Recombinant Proteins / Q000378:metabolism; D018411:Spodoptera; D013379:Substrate Specificity; D014162:Transfection
D018076:DNA, Complementary; D004075:Diglycerides; D010940:Plant Proteins; D011994:Recombinant Proteins; C043222:patatin protein, Solanum tuberosum; D002265:Carboxylic Ester Hydrolases; C019262:galactolipase