title: Construction of a human cardiovascular cDNA microarray: portrait of the failing heart.
abstract: Identifying key genes that regulate the complex diseases of the cardiovascular system can be greatly facilitated with the use of microarrays. In an effort to obtain a global portrait of gene expression in the failing heart, we have constructed in-house a glass microscope slide cDNA microarray (termed "CardioChip") containing 10,368 redundant and randomly-selected sequenced expressed sequence tags (representing known genes, other matched ESTs, and novel, unmatched ESTs) derived from several human heart and artery cDNA libraries. From our preliminary data with Cy3- and Cy5-labeled probes, we have identified 38 transcripts showing a minimum twofold differential expression, among which are several novel or previously-uncharacterized genes. This array-representing what we believe to be the largest cardiovascular-based cDNA array to date-establishes a practical and invaluable platform for obtaining a global genetic portrait of complex cardiovascular diseases, particularly in the failing heart.
authors.affiliation: The Cardiovascular Genome Unit, Brigham and Women's Hospital, Boston, Massachusetts, USA.
authors.forename: J D
authors.initials: JD
authors.lastname: Barrans
authors.forename: D
authors.initials: D
authors.lastname: Stamatiou
authors.forename: C
authors.initials: C
authors.lastname: Liew
mesh_terms: D001158:Arteries / Q000378:metabolism; D002319:Cardiovascular System / Q000378:metabolism / Q000503:physiopathology*; D018076:DNA, Complementary / Q000378:metabolism; D020224:Expressed Sequence Tags; D015870:Gene Expression; D006321:Heart / Q000503:physiopathology*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D009206:Myocardium / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger