title: Integrin-mediated interactions between human bone marrow stromal precursor cells and the extracellular matrix.
abstract: To date, the precise interactions between bone marrow stromal cells and the extracellular matrix that govern stromal cell development remain unclear. The integrin super-family of cell-surface adhesion molecules represents a major pathway used by virtually all cell types to interact with different extracellular matrix components. In this study, purified populations of stromal precursor cells were isolated from the STRO-1-positive fraction of normal human marrow, by fluoresence-activated cell sorting, and then assayed for their ability to initiate clonogenic growth in the presence of various integrin ligands. Bone marrow-derived stromal progenitors displayed differential growth to fibronectin, vitronectin, and laminin, over collagen types I and III, but showed a similar affinity for collagen type IV. The integrin heterodimers alpha1beta1, alpha2beta1, alpha5beta1, alpha6beta1, alpha(v)beta3, and alpha(v)beta5 were found to coexpress with the STRO-1 antigen on the cell surface of CFU-F, using dual-color analysis. Furthermore, only a proportion of stromal precursors expressed the integrin alpha4beta1, while no measurable levels of the integrin alpha3beta1 could be detected. Subsequent adhesion studies using functional blocking antibodies to different integrin alpha/beta heterodimers showed that stromal cell growth on collagen, laminin, and fibronectin was mediated by multiple beta1 integrins. In contrast, cloning efficiency in the presence of vitronectin was mediated in part by alpha(v)beta3. When human marrow stromal cells were cultured under osteoinductive conditions, their ability to form a mineralized matrix in vitro was significantly diminished in the presence of a functional blocking monoclonal antibody to the beta1 integrin subunit. The results of this study indicate that beta1 integrins appear to be the predominant adhesion receptor subfamily utilized by stromal precursor cells to adhere and proliferate utilizing matrix glycoproteins commonly found in the bone marrow microenvironment and bone surfaces. Furthermore, these data suggest a possible role for the beta1 integrin subfamily during the development of stromal precursor cells into functional osteoblast-like cells.
authors.affiliation: Craniofacial & Skeletal Diseases Branch, National Institute of Dental & Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA. sgronthos@dir.nidcr.nih.gov
authors.forename: S
authors.initials: S
authors.lastname: Gronthos
authors.forename: P J
authors.initials: PJ
authors.lastname: Simmons
authors.forename: S E
authors.initials: SE
authors.lastname: Graves
authors.forename: P G
authors.initials: PG
authors.lastname: Robey
mesh_terms: D000911:Antibodies, Monoclonal; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000187:drug effects; D003094:Collagen / Q000494:pharmacology; D005109:Extracellular Matrix / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000494:pharmacology; D005353:Fibronectins / Q000494:pharmacology; D005434:Flow Cytometry; D006801:Humans; D039222:Integrin alpha1beta1; D039121:Integrin alpha6beta1; D016023:Integrins / Q000276:immunology / Q000378:metabolism*; D007797:Laminin / Q000494:pharmacology; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D038981:Receptors, Collagen; D017469:Receptors, Fibronectin / Q000276:immunology / Q000378:metabolism; D019030:Receptors, Vitronectin / Q000276:immunology / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism*; D019096:Vitronectin / Q000494:pharmacology
chemical_list: D000911:Antibodies, Monoclonal; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D039222:Integrin alpha1beta1; D039121:Integrin alpha6beta1; D016023:Integrins; D007797:Laminin; D038981:Receptors, Collagen; D017469:Receptors, Fibronectin; D019030:Receptors, Vitronectin; D019096:Vitronectin; C064660:integrin alphaVbeta5; D003094:Collagen