Interphase movements of a DNA chromosome region modulated by VP16 transcriptional activator.
We examined changes in intranuclear chromosome positioning induced by a transcriptional activator in a simple experimental system. Targeting the VP16 acidic activation domain (AAD) to an engineered chromosome site resulted in its transcriptional activation and redistribution from a predominantly peripheral to a more interior nuclear localization. Direct visualization in vivo revealed that the chromosome site normally moves into the nuclear interior transiently in early G1 and again in early S phase. In contrast, VP16 AAD targeting induced this site's permanent interior localization in early G1. A single transcriptional activator therefore can modify the cell-cycle-dependent programme of intranuclear positioning of chromosome loci.
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Department of Cell and Structural Biology, University of Illinois at Urbana-Champaign, Room B107 C&LSL, 601 South Goodwin Avenue, Urbana, Illinois 61801, USA.
Belmont
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D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism*; D002875:Chromosomes / Q000235:genetics* / Q000378:metabolism; D006224:Cricetinae; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D017930:Genes, Reporter / Q000235:genetics; D017405:Herpes Simplex Virus Protein Vmw65 / Q000235:genetics* / Q000378:metabolism; D007399:Interphase; D008856:Microscopy, Fluorescence; D017434:Protein Structure, Tertiary; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D015533:Transcriptional Activation / Q000235:genetics
D017405:Herpes Simplex Virus Protein Vmw65; D011993:Recombinant Fusion Proteins; D004247:DNA