Two-dimensional crystallogenesis of transmembrane proteins.
Two-dimensional crystallogenesis is a crucial step in the long road that leads to the determination of macromolecules structure via electron crystallography. The necessity of having large and highly ordered samples can hold back the resolution of structural works for a long time, and this, despite improvements made in electron microscopes or image processing. Today, finding good conditions for growing two-dimensional crystals still rely on either "biocrystallo-cooks" or on lucky ones. The present review presents the field by first describing the different crystals that one can encounter and the different crystallisation methods used. Then, the effects of different components (such as protein, lipids, detergent, buffer, and temperature) and the different methods (dialysis, hydrophobic adsorption) are discussed. This discussion is punctuated by correspondences made to the world of three-dimensional crystallogenesis. Finally, a guide for setting up 2D crystallogenesis experiments, built on the discussion mentioned before, is proposed to the reader. More than giving recipes, this review is meant to open up the discussions in this field.
Mosser
G
G
LPCC, UMR168-CNRS, Institut Curie-Section de Recherche, 11 rue Pierre et Marie Curie, 75005 Paris, France. gervaise.mosser@lps.ens.fr
D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry; D002021:Buffers; D003461:Crystallography / Q000379:methods*; D003902:Detergents; D004583:Electrons; D006801:Humans; D008563:Membrane Lipids / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry*; D008958:Models, Molecular; D017410:Practice Guidelines as Topic; D013696:Temperature; D016707:Tissue Fixation / Q000379:methods
D001426:Bacterial Proteins; D002021:Buffers; D003902:Detergents; D008563:Membrane Lipids; D008565:Membrane Proteins