Role of cytomegalovirus replication in alopecia areata pathogenesis.
BACKGROUND
Cytomegalovirus (CMV) infection has been correlated with various autoimmune disorders. Using molecular biology techniques, DNA sequences of CMV have been reported in paraffin sections of alopecia areata (AA) lesions. Reactivation of the CMV infection has been postulated as one of the pathogenic mechanisms in AA. Other studies, using different techniques however have demonstrated no correlation between CMV and AA.


OBJECTIVE
This study was to clarify the role of CMV infection and to demonstrate the absence of replication of other autoimmune diseases-related herpes virus (EBV) in the pathogenesis of AA.


METHODS
After extraction of mRNA from tissue samples of a patient with active patchy AA, reverse transcriptase-polymerase chain reaction was carried out using primers specific for some viral members of the beta-herpes viridae family (CMV, EBV, HSV).


RESULTS
No replication of the CMV or other beta-herpes viridae has been detected in any of the samples collected.


CONCLUSIONS
The results strongly support the hypothesis that CMV is not the triggering factor in AA, neither as a re-activator of the immune response nor as a trigger of the autoimmunity. No other herpes virus is implicated in the pathogenesis of this disease.
Offidani
A
A
Department of Dermatology, University of Ancona, Ancona, Italy.
Amerio
P
P
Bernardini
M L
ML
Feliciani
C
C
Bossi
G
G
D000328:Adult; D000506:Alopecia Areata / Q000209:etiology* / Q000821:virology; D001706:Biopsy; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D003586:Cytomegalovirus Infections / Q000150:complications*; D017931:DNA Primers; D018076:DNA, Complementary / Q000235:genetics; D004279:DNA, Viral / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D005260:Female; D006564:Herpesviridae / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D006801:Humans; D008297:Male; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000821:virology; D014779:Virus Replication
D017931:DNA Primers; D018076:DNA, Complementary; D004279:DNA, Viral