title: Crystallization and preliminary X-ray analysis of the sporulation factor SpoIIAA in its native and phosphorylated forms.
abstract: Sporulation in Bacillus begins with an asymmetric cell division producing two progeny with identical chromosomes but different developmental fates. As such, it is a simple example of cellular differentiation. The establishment of cell type is controlled by a series of alternate RNA polymerase sigma subunits. The first compartment-specific sigma factor is sigma(F), whose activity is controlled by SpoIIAB, an anti-sigma factor, and SpoIIAA, an anti-sigma factor antagonist which is phosphorylated by the kinase activity of SpoIIAB. Here, the preliminary crystallographic analysis of SpoIIAA and phosphorylated SpoIIAA from B. sphaericus in forms suitable for high-resolution structure determination are reported.
authors.affiliation: Structural Biology Laboratory, Department of Chemistry, University of York, York YO10 5DD, England.
authors.forename: P R
authors.initials: PR
authors.lastname: Seavers
authors.forename: R J
authors.initials: RJ
authors.lastname: Lewis
authors.forename: J A
authors.initials: JA
authors.lastname: Brannigan
authors.forename: A J
authors.initials: AJ
authors.lastname: WilkinsonJ
mesh_terms: D001407:Bacillus / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000302:isolation & purification; D001411:Geobacillus stearothermophilus / Q000235:genetics; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D012808:Sigma Factor; D014157:Transcription Factors
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D011994:Recombinant Proteins; D012808:Sigma Factor; D014157:Transcription Factors; C092468:spoIIR protein, Bacillus subtilis; C030069:spore-specific proteins, Bacillus