A simple method for the measurement of sjTREC levels in blood.
We have developed a relatively rapid, safe and simple method for the quantification of sjTREC levels in samples of peripheral blood. The assay uses an image analysis package to measure the brightness of PCR product bands on an image of the standard agarose gel. Comparison of the brightness of the band with that obtained from a standard curve provides a read-out of the amount of sjTRECs in the sample. We have compared the sjTREC levels we obtained with this method with those obtained from real time analysis PCR using a Lightcycler and found that they are comparable.
Aspinall
R
R
Department of Immunology, Imperial College School of Medicine, Chelsea and Westminister Hospital, 369 Fulham Road, London SW10 9NH, UK. r.aspinall@ic.ac.uk
Pido
J
J
Andrew
D
D
D000328:Adult; D000375:Aging / Q000097:blood; D001769:Blood / Q000378:metabolism*; D004260:DNA Repair; D005260:Female; D015321:Gene Rearrangement; D019672:Genes, T-Cell Receptor; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007156:Immunologic Memory; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D013601:T-Lymphocytes / Q000378:metabolism / Q000502:physiology
D016693:Receptors, Antigen, T-Cell, alpha-beta