Effects of enamel matrix derivative on gene expression of primary osteoblasts.
OBJECTIVE
The aim of this study was to investigate the effects of enamel matrix derivative (EMD) on gene expression of collagen alpha1 (I), osteocalcin, prostaglandin G/H synthase 2 (PGHS-2), interleukin-6, and insulin-like growth factor I in primary mouse osteoblasts.


METHODS
Primary osteoblasts were digested from 6- to 8-day-old mouse calvaria. Cells were divided into 4 groups and cultured for 24, 48, and 72 hours with a serum-free modified Eagle medium as negative control, modified Eagle medium with 25 microg/mL EMD, modified Eagle medium with 100 microg/mL EMD, and modified Eagle medium plus 10% fetal bovine serum as positive control. Gene expression was determined by Northern blot and reverse transcription polymerase chain reaction analysis.


RESULTS
EMD enhanced collagen I, interleukin-6, and PGHS-2 expression and did not stimulate the expression of osteocalcin and IGF-I.


CONCLUSIONS
These results indicate that EMD might regulate certain gene expression during periodontal tissue regeneration.
Jiang
J
J
Department of Endodontology, School of Dental Medicine, University of Connecticut Health Center, Farmington, USA.
Fouad
A F
AF
Safavi
K E
KE
Spångberg
L S
LS
Zhu
Q
Q
D000818:Animals; D015152:Blotting, Northern; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D003746:Dental Enamel Proteins / Q000494:pharmacology*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D015675:Osteocalcin / Q000096:biosynthesis; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D003746:Dental Enamel Proteins; D015850:Interleukin-6; C109069:enamel matrix proteins; D015675:Osteocalcin; D007334:Insulin-Like Growth Factor I; D003094:Collagen; D011451:Prostaglandin-Endoperoxide Synthases