title: The three-dimensional structure of cytosolic bovine retinal creatine kinase.
abstract: Creatine kinase (CK) catalyses the reversible transfer of the phosphate moiety from phosphocreatine (PCr) to ADP, generating creatine and ATP. The crystal structure of a cytosolic brain-type creatine kinase is reported at 2.3 A. The biological dimer sits on a crystallographic twofold axis. The N-terminal residues of both subunits come very close to the crystallographic twofold at the dimer interface. The electron density observed is consistent with two alternative conformations for the N-termini, as previously found for chicken brain-type creatine kinase.
authors.affiliation: Department of Crystallography, Birkbeck College, Malet Street, London WC1 7HX, England.
authors.forename: D
authors.initials: D
authors.lastname: Tisi
authors.forename: B
authors.initials: B
authors.lastname: Bax
authors.forename: A
authors.initials: A
authors.lastname: Loew
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000201:enzymology; D002417:Cattle; D002645:Chickens; D003402:Creatine Kinase / Q000737:chemistry*; D018360:Crystallography, X-Ray; D003600:Cytosol / Q000201:enzymology; D019281:Dimerization; D006801:Humans; D007527:Isoenzymes / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010786:Photoreceptor Cells / Q000201:enzymology; D017433:Protein Structure, Secondary; D021122:Protein Subunits; D012160:Retina / Q000201:enzymology*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D055672:Static Electricity
chemical_list: D007527:Isoenzymes; D021122:Protein Subunits; D003402:Creatine Kinase