title: Ultrasound-induced formation of S-nitrosoglutathione and S-nitrosocysteine in aerobic aqueous solutions of glutathione and cysteine.
abstract: S-Nitrosocompounds are formed when aqueous solutions of cysteine or glutathione are exposed to ultrasound (880 kHz) in air. The yield of the S-nitrosocompounds was as high as 10% for glutathione and 4% for cysteine of the initial thiol concentrations (from 0.1 to 10 mM) in the aqueous solutions. In addition to the formation of S-nitrosocompounds, thiol oxidation to disulfide forms was observed. After the oxidation of over 70% of the sulfhydryl groups, formation of peroxide compounds as well as cysteic acid derivatives was recorded. The formation of the peroxide compounds and peroxide radicals in the ultrasound field reduced the yield of S-nitrosocompounds. S-Nitrosocompounds were not formed when exposing low-molecular-weight thiols to ultrasound in atmospheres of N(2) or CO. In neutral solutions, ultrasound-exposed cysteine or glutathione released NO due to spontaneous degradation of the S-nitrosocompounds. N(2)O(3), produced due to the spontaneous degradation of the S-nitrosocompounds in air, nitrosylated sulfhydryl groups of glutathione manifested in the appearance of new absorption bands at 330 and 540 nm. The nitrogen compounds formed in an ultrasound field modified the sulfhydryl groups of apohemoglobin and serum albumin. The main target for ultrasound-generated oxygen free radicals were cystine residues oxidized to cysteic acid residues.
authors.affiliation: Institute of Biochemistry, National Academy of Sciences of Belarus, Grodno, 230017 Belarus. stepuro@grsu.grodno.by
authors.forename: I I
authors.initials: II
authors.lastname: Stepuro
authors.forename: R I
authors.initials: RI
authors.lastname: Adamchuk
authors.forename: T P
authors.initials: TP
authors.lastname: Piletskaya
authors.forename: V I
authors.initials: VI
authors.lastname: Stepuro
authors.forename: S A
authors.initials: SA
authors.lastname: Maskevich
mesh_terms: D000388:Air; D003545:Cysteine / Q000031:analogs & derivatives* / Q000737:chemistry*; D005609:Free Radicals; D005978:Glutathione / Q000031:analogs & derivatives* / Q000737:chemistry*; D007700:Kinetics; D008956:Models, Chemical; D009569:Nitric Oxide / Q000378:metabolism; D009603:Nitroso Compounds / Q000737:chemistry*; D010100:Oxygen / Q000378:metabolism; D010545:Peroxides / Q000378:metabolism; D026422:S-Nitrosoglutathione; D026403:S-Nitrosothiols; D013997:Time Factors; D014463:Ultrasonography; D014867:Water / Q000378:metabolism*
chemical_list: D005609:Free Radicals; D009603:Nitroso Compounds; D010545:Peroxides; D026403:S-Nitrosothiols; D014867:Water; D009569:Nitric Oxide; D026422:S-Nitrosoglutathione; C021315:S-nitrosocysteine; D005978:Glutathione; D003545:Cysteine; D010100:Oxygen