Prevention of entrance into G2 cell cycle phase by mimosine decreases locomotion of cells from the tumor cell line SW480.
Cellular proliferation of tumor cells is thought to impede migratory activity. Using continuous single cell migration analysis of the colon carcinoma cell line SW480 for up to 72 h, we were able to show that cells locomote constantly and stop only for actual cell division. These findings indicate that proliferation (from G1 phase to early mitosis) and migration do occur simultaneously. The presence of the cell cycle marker Ki-67 in individual migrating cells substantiated this observation. Inhibition of cell cycle progression by mimosine (MIM), a reversible cell cycle blocker, reduced the percentage of migrating cells; release from MIM block restored migratory capacity. The corresponding cell cycle phase distributions were confirmed by flow cytometry. In our test system cell cycle events and migration were shown to occur at the same time. Interference with cell cycle progression reduced migratory activity indicating that migration depends on an unhampered cell cycle.
Kubens
B S
BS
Institute of Immunology, University Witten/Herdecke, Stockumer Strasse, 10, 58448 Witten, Germany. bks@uni-wh.de
Niggemann
B
B
Zänker
K S
KS
D002453:Cell Cycle / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D005434:Flow Cytometry; D016195:G2 Phase / Q000187:drug effects*; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis; D018613:Microscopy, Confocal; D018715:Microscopy, Video; D008898:Mimosine / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D019394:Ki-67 Antigen; D008898:Mimosine; D004247:DNA