Effects of leuprolide acetate on adhesion formation and wound healing in the rat model.
OBJECTIVE
To assess the effects of a hypoestrogenic state on adhesion formation and reformation and on wound healing in the rat model.


METHODS
Prospective, randomized study (Canadian Task Force classification I).


METHODS
Forty-eight female Sprague-Dawley rats.


METHODS
Rats were injected with gonadotropin-hormone releasing hormone (GnRH) agonist and control rats with normal saline. Two weeks later (day zero) laparotomy was performed to create adhesions and a full-thickness wound on the flank. On day 14 the adhesions were scored and liberated. The rats were sacrificed on day 28 and adhesion reformation was evaluated. The wound surface area was measured serially until complete closure.


RESULTS
Mean adhesion scores on day 14 after adhesion formation were not significantly different between GnRH agonist and control groups. Preadhesiolysis and postadhesiolysis scores were not significantly different. There was no significant difference in size of wounds between groups on days zero, 7, or 14.


CONCLUSIONS
Administration of a GnRH agonist does not seem to influence postoperative adhesion formation or wound healing in the rat model. (J Am Assoc Gynecol Laparosc 8(1):124-128, 2001)
Roberts
J E
JE
Department of Obstetrics and Gynecology, Women's Pavilion, Royal Victoria Hospital, 2058 Jeanne Mance, Montreal, Quebec.
Watkin
K L
KL
Tulandi
T
T
D000818:Animals; D005260:Female; D005300:Fertility Agents, Female / Q000494:pharmacology*; D005831:Genital Diseases, Female / Q000503:physiopathology / Q000517:prevention & control; D013509:Gynecologic Surgical Procedures; D016729:Leuprolide / Q000494:pharmacology*; D011184:Postoperative Period; D051381:Rats; D017207:Rats, Sprague-Dawley; D000267:Tissue Adhesions / Q000503:physiopathology / Q000517:prevention & control; D014945:Wound Healing / Q000187:drug effects*
D005300:Fertility Agents, Female; D016729:Leuprolide