Changes in detrusor and urinary growth factors according to detrusor function after partial bladder outlet obstruction in the rat.
OBJECTIVE
To investigate the changes in detrusor and urinary growth factors according to detrusor function after partial bladder outlet obstruction in the rat. Growth factors have been reported to play a role in the remodeling of the bladder after obstruction.


METHODS
Female rats (n = 40) were used and divided into three groups: sham-operated control, 2-week mild obstruction, and 2-week severe obstruction. The voided urine was collected and the detrusor contractility to electrical stimulation recorded. mRNA expression and the urinary concentration of transforming growth factor beta-1 (TGF-beta1) and basic fibroblast growth factor (bFGF) were analyzed.


RESULTS
The contractile response to electrical stimulation was increased in the mild obstruction group (P <0.05) but decreased in the severe obstruction group (P <0.05). The detrusor mRNA expression of TGF-beta1 in the mild obstruction group was not different from that in the control group. However, in the severe obstruction group, the mRNA expression of TGF-beta1 increased. The detrusor mRNA expression of bFGF was gradually increased in the mild and severe obstruction groups. The urinary concentration of TGF-beta1 in the mild obstruction group was not significantly different from that in the control group. However, in the severe obstruction group, the concentration of TGF-beta1 was significantly increased (P <0.05). The concentration of urinary bFGF was too small to compare the results in each group.


CONCLUSIONS
Growth factors such as TGF-beta1 and bFGF change in mRNA expression according to bladder function, and urinary TGF-beta1 reflects the change of detrusor mRNA. Thus, changes in TGF-beta1 may be a marker to evaluate detrusor function.
Chul Kim
J
J
Department of Urology, Catholic University of Korea College of Medicine, Seoul, South Korea.
Il Seo
S
S
Hyun Park
Y
Y
Kon Hwang
T A
TA
D000818:Animals; D004558:Electric Stimulation; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000652:urine*; D009119:Muscle Contraction / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D016212:Transforming Growth Factor beta / Q000652:urine*; D053773:Transforming Growth Factor beta1; D001748:Urinary Bladder Neck Obstruction / Q000503:physiopathology* / Q000652:urine
D012333:RNA, Messenger; C508962:Tgfb1 protein, rat; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D016222:Fibroblast Growth Factor 2