Altered expression of CD88 and associated impairment of complement 5a-induced neutrophil responses in human immunodeficiency virus type 1-infected patients with and without pulmonary tuberculosis.
The effect of infection with human immunodeficiency virus type 1 (HIV patient group), infection with Mycobacterium tuberculosis (TB patient group), and coinfection with both of these organisms (HIV/TB patient group) on the expression of CD88 on polymorphonuclear leukocytes (PMNL) was determined by using a receptor-specific monoclonal antibody and flow cytometry. A significant reduction in the fluorescence intensity of CD88 on PMNL was observed in the HIV and HIV/TB groups, compared with both the healthy donor (HD) and TB groups. Furthermore, when degranulation of PMNL was induced by ligation of CD88 by complement 5a (C5a), a large proportion of patients in the HIV and the HIV/TB groups was found to have reciprocal degranulation responses. Patients in the 2 HIV groups also were found to have significantly reduced C5a-induced chemotactic responses and significantly elevated peripheral levels of C5a des Arg, compared with the HD and TB groups. These differences may contribute to the increased susceptibility of HIV-1-infected individuals to secondary microbial infections.
Meddows-Taylor
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AIDS Virus Research Unit, National Institute for Virology, Sandringham, 2131, South Africa. stephent@niv.ac.za
Pendle
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Tiemessen
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D017088:AIDS-Related Opportunistic Infections / Q000209:etiology / Q000276:immunology; D000328:Adult; D015703:Antigens, CD / Q000378:metabolism*; D015550:Cell Degranulation; D002634:Chemotaxis, Leukocyte; D015936:Complement C5a / Q000276:immunology*; D015937:Complement C5a, des-Arginine / Q000032:analysis; D005434:Flow Cytometry; D005966:Glucuronidase / Q000378:metabolism; D015658:HIV Infections / Q000150:complications / Q000276:immunology* / Q000821:virology; D015497:HIV-1 / Q000302:isolation & purification; D006801:Humans; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000276:immunology; D009504:Neutrophils / Q000276:immunology*; D012367:RNA, Viral / Q000097:blood; D044087:Receptor, Anaphylatoxin C5a; D011951:Receptors, Complement / Q000378:metabolism*; D014397:Tuberculosis, Pulmonary / Q000150:complications / Q000276:immunology* / Q000382:microbiology
D015703:Antigens, CD; D015937:Complement C5a, des-Arginine; D012367:RNA, Viral; D044087:Receptor, Anaphylatoxin C5a; D011951:Receptors, Complement; D015936:Complement C5a; D005966:Glucuronidase