[Detection of human papillomavirus in Chinese laryngeal carcinoma with digoxigenin labelled probe prepared by PCR].
The studies described here detect HPV DNA in 124 cases of fresh tissue samples with different lesions of larynx using a nonradioactive Digoxigenin Labelled Probe prepared by polymerase chain reaction (PCR). The labelled probe of HPV consensus primers was obtained, which can detect nine types of HPVs DNAs such as HPV 6, 11, 16, 18, 31, 33, 35, 42 and 58. The results showed that the rate of positive cases with HPV infection was 45.6% (31/68) in the group of laryngeal carcinoma, 20.0% (3/15) for the group of neck metastatic lymph node, 11.8% (2/17) for precarcinous lesions group and 6.3% (1/16) for the group of vocal cord polypus, respectively. Fifteen cases of normal laryngeal tissue adjacent to the carcinoma and 15 cases adjacent to the carcinoma were HPV DNA negative. The carcinogenic action of HPV in laryngeal carcinoma and the sensitivity and specificity of the probe prepared by PCR are discussed in this paper.
Zhao
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Department of Otorhinolaryngology, Changzheng Hospital, Second Millitary Medical University, Shanghai 200003.
Lu
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S
Fei
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D004279:DNA, Viral / Q000032:analysis; D004076:Digoxigenin; D006801:Humans; D007822:Laryngeal Neoplasms / Q000821:virology*; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections; D016133:Polymerase Chain Reaction; D011230:Precancerous Conditions / Q000821:virology; D014412:Tumor Virus Infections
D004279:DNA, Viral; D004076:Digoxigenin