Transgenic oil palm: production and projection.
Oil palm is an important economic crop for Malaysia. Genetic engineering could be applied to produce transgenic oil palms with high value-added fatty acids and novel products to ensure the sustainability of the palm oil industry. Establishment of a reliable transformation and regeneration system is essential for genetic engineering. Biolistic was initially chosen as the method for oil palm transformation as it has been the most successful method for monocotyledons to date. Optimization of physical and biological parameters, including testing of promoters and selective agents, was carried out as a prerequisite for stable transformation. This has resulted in the successful transfer of reporter genes into oil palm and the regeneration of transgenic oil palm, thus making it possible to improve the oil palm through genetic engineering. Besides application of the Biolistics method, studies on transformation mediated by Agrobacterium and utilization of the green fluorescent protein gene as a selectable marker gene have been initiated. Upon the development of a reliable transformation system, a number of useful targets are being projected for oil palm improvement. Among these targets are high-oleate and high-stearate oils, and the production of industrial feedstock such as biodegradable plastics. The efforts in oil palm genetic engineering are thus not targeted as commodity palm oil. Due to the long life cycle of the palm and the time taken to regenerate plants in tissue culture, it is envisaged that commercial planting of transgenic palms will not occur any earlier than the year 2020.
Parveez
G K
GK
Malaysian Palm Oil Board (MPOB), P.O. Box 10620, 50720 Kuala Lumpur, Malaysia. parveez@mpob.gov.my
Masri
M M
MM
Zainal
A
A
Majid
N A
NA
Yunus
A M
AM
Fadilah
H H
HH
Rasid
O
O
Cheah
S C
SC
D019470:Biolistics / Q000379:methods; D005819:Genetic Markers; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000032:analysis / Q000235:genetics; D019301:Oleic Acid / Q000032:analysis*; D000073878:Palm Oil; D010938:Plant Oils / Q000737:chemistry; D010945:Plants, Edible / Q000235:genetics* / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000378:metabolism; D012231:Rhizobium; D013229:Stearic Acids / Q000032:analysis*
D005819:Genetic Markers; D008164:Luminescent Proteins; D010938:Plant Oils; D013229:Stearic Acids; D049452:Green Fluorescent Proteins; D019301:Oleic Acid; C031183:stearic acid; D000073878:Palm Oil