Synthesis and in vitro evaluation of 123I-labelled human recombinant annexin V.
Annexin V was radiolabelled with iodine-123 in order to develop a SPECT-ligand for imaging atherosclerosis and apoptosis. Iodination by means of electrophylic substitution resulted in radiochemical yields up to 70% and specific activities of 7.4-92.5 MBq/microg protein. Binding experiments with blood platelets indicated that 123I-labelled annexin V remained its biological activity.
Lahorte
C
C
Department of Radiopharmacy, Faculty of Pharmaceutical Sciences, Harelbekestraat 72, B-9000, Ghent, Belgium. christophe.lahorte@rug.ac.be
Slegers
G
G
Philippé
J
J
Van de Wiele
C
C
Dierckx
R A
RA
D017304:Annexin A5 / Q000138:chemical synthesis*; D001792:Blood Platelets / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007457:Iodine Radioisotopes / Q000737:chemistry; D008024:Ligands; D011485:Protein Binding; D011994:Recombinant Proteins / Q000138:chemical synthesis*; D013997:Time Factors; D015899:Tomography, Emission-Computed, Single-Photon / Q000379:methods
D017304:Annexin A5; D007457:Iodine Radioisotopes; D008024:Ligands; D011994:Recombinant Proteins