title: Selectivity in citric acid production by Yarrowia lipolytica.
abstract: This experimental study reports about production selectivity in the fermentation of glucose to citric acid by Yarrowia lipolytica as a function of substrate concentration. Batch runs featuring biomass growth and one or two citric acid production phases were carried out in a 15-l stirred tank fermentor. The presented results demonstrate that working at high initial substrate concentration in the production phase is beneficial both in terms of a higher production rate of citric acid, the desired metabolite (reaching 0.077 h(-1)) and of a higher utilization degree of the employed carbon source (yield up to 0.384 g(c.a.)/g(glucose)). The production rate of isocitric acid, the major undesired metabolite, was found to be practically constant over the tested initial substrate concentration range.
authors.affiliation: Dipartimento di Ingegneria Chimica, Università di Roma "La Sapienza", Italy
authors.forename: S
authors.initials: S
authors.lastname: Antonucci
authors.forename: M
authors.initials: M
authors.lastname: Bravi
authors.forename: R
authors.initials: R
authors.lastname: Bubbico
authors.forename: A
authors.initials: A
authors.lastname: Di Michele
authors.forename: N
authors.initials: N
authors.lastname: Verdone