title: Expression of hemin receptor molecule ChuA is influenced by RfaH in uropathogenic Escherichia coli strain 536.
abstract: The outer membrane protein ChuA responsible for hemin utilization has been recently identified in several pathogenic Escherichia coli strains. We report that the regulatory protein RfaH influences ChuA expression in the uropathogenic E. coli strain 536. In an rfaH mutant, the chuA transcript as well as the ChuA protein levels were significantly decreased in comparison with those in the wild-type strain. Within the chuA gene, a consensus motif known as the JUMPStart (just upstream of many polysaccharide associated gene starts) sequence was found, which is shared by RfaH-affected operons. Furthermore, the presence of two different subclasses of the chuA determinant and their distribution in E. coli pathogroups are described.
authors.affiliation: Institut für Molekulare Infektionsbiologie, Universität Würzburg, 97070 Würzburg, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Nagy
authors.forename: U
authors.initials: U
authors.lastname: Dobrindt
authors.forename: M
authors.initials: M
authors.lastname: Kupfer
authors.forename: L
authors.initials: L
authors.lastname: Emödy
authors.forename: H
authors.initials: H
authors.lastname: Karch
authors.forename: J
authors.initials: J
authors.lastname: Hacker
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics / Q000472:pathogenicity*; D029968:Escherichia coli Proteins; D019143:Evolution, Molecular; D006427:Hemin / Q000378:metabolism*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009876:Operon; D010445:Peptide Elongation Factors / Q000235:genetics*; D011704:Pyelonephritis / Q000382:microbiology*; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000235:genetics; D017422:Sequence Analysis, DNA; D015534:Trans-Activators / Q000235:genetics*
chemical_list: D001425:Bacterial Outer Membrane Proteins; C423457:ChuA protein, E coli; D029968:Escherichia coli Proteins; D010445:Peptide Elongation Factors; D011956:Receptors, Cell Surface; C106401:RfaH protein, E coli; D015534:Trans-Activators; D006427:Hemin