title: Improved immune functions with administration of a low-fat diet in a burn animal model.
abstract: The purpose of this study was to characterize the impact of a low-fat (LF; 1% fat) diet, a high-fat (HF; 25% fat) diet, and a standard (SD; 5% fat) diet on immune and oxidative parameters in a 20% body surface area burn animal model fed ad libitum for 10 days postinjury. Although the mechanisms are poorly understood, the amount of dietary lipid in nutritional support has been shown to have immunomodulatory effects after burn injury. Burned mice fed the LF diet showed a normal response in activated splenocyte proliferation compared to burned animals that received the SD or HF diet. Animals fed the SD and HF diets presented increased production of nitric oxide and prostaglandin E2 response after burn injury, which is associated with inhibited splenocyte proliferation. The total thiol concentration in spleen cells from burned animals kept on the HF diet was significantly higher than that in unburned animals, while no increase in these oxidative parameters was observed in LF-fed burned animals. Moreover, the LF diet significantly reduced hepatic lipid peroxidation, as measured by malonaldehyde concentration, compared to the other two diets. These results suggest that the administration of a LF diet in mice after a burn injury prevents inhibition of in vitro splenocyte proliferation and reduces the intensity of oxidative stress.
authors.affiliation: Centre des Grands Brûlés, Centre Hospitalier de l'Université de Montréal (CHUM)-Hôtel-Dieu, Montréal, Quebec, Canada.
authors.forename: N
authors.initials: N
authors.lastname: Jobin
authors.forename: D R
authors.initials: DR
authors.lastname: Garrel
authors.forename: J
authors.initials: J
authors.lastname: Champoux
authors.forename: J
authors.initials: J
authors.lastname: Bernier
mesh_terms: D000821:Animal Feed; D000818:Animals; D002056:Burns / Q000178:diet therapy* / Q000276:immunology* / Q000378:metabolism; D016516:CD4-CD8 Ratio; D003208:Concanavalin A / Q000494:pharmacology; D003314:Corn Oil / Q000008:administration & dosage; D018752:Diet, Fat-Restricted; D004041:Dietary Fats / Q000008:administration & dosage; D015232:Dinoprostone / Q000096:biosynthesis; D004195:Disease Models, Animal; D004435:Eating / Q000276:immunology; D005260:Female; D007106:Immune Sera / Q000494:pharmacology; D016130:Immunophenotyping; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D051379:Mice; D008809:Mice, Inbred C3H; D009569:Nitric Oxide / Q000096:biosynthesis; D018384:Oxidative Stress / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D015430:Weight Gain / Q000276:immunology
chemical_list: D004041:Dietary Fats; D007106:Immune Sera; D008070:Lipopolysaccharides; D011948:Receptors, Antigen, T-Cell; D003208:Concanavalin A; D009569:Nitric Oxide; D003314:Corn Oil; D015232:Dinoprostone; C029310:lard