[Proliferative activity and chromosomal alterations of smooth muscle cells in atherosclerosis].
Atherosclerosis is the most frequent cause of death in industrialized countries. Lesions are characterized by lipid deposits, focal thickening of the arterial wall with proliferation of smooth muscle cells (SMC), mononuclear infiltrates and neoformed vessels. In this paper, we studied the proliferative characteristics and cytogenetic alterations of SMC. These cells, expressing specific muscular actin, were diploid with an increased proliferative index for PCNA. A high percentage of SMC showed intense expression of p53. There were signs of chromosomal instability, being the most frequent findings chromosome 7 trisomy and chromosome 11 monosomy. Additionally, the gene for FGF-3 showed a marked amplification. These findings strongly suggest that SMC proliferation is active, and is related to the accumulation or mutation of the p53 oncoprotein. It also presents specific chromosomal alterations in close relation with growth factors. According to these findings SMC hyperplasia in the atherosclerosis plaque may be considered as a cellular clonal expansion.
Fernández Alonso
G
G
Diagnóstico Maipú, Facultad de Medicina, Universidad del Salvador, Argentina.
Grana
D R
DR
Turconi
P
P
Colombo
B
B
Lavezzi
A M
AM
Milei
J
J
Matturri
L
L
D001161:Arteriosclerosis / Q000235:genetics* / Q000473:pathology; D002906:Chromosomes, Human, 6-12 and X / Q000235:genetics*; D002880:Chromosomes, Human, Pair 11 / Q000378:metabolism; D002897:Chromosomes, Human, Pair 7 / Q000235:genetics; D051518:Fibroblast Growth Factor 3; D005346:Fibroblast Growth Factors / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D009130:Muscle, Smooth / Q000473:pathology; D009687:Nuclear Proteins / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics; D014314:Trisomy; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
C496444:FGF3 protein, human; D051518:Fibroblast Growth Factor 3; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; D016159:Tumor Suppressor Protein p53; D005346:Fibroblast Growth Factors