Parietal endoderm cell line from a rat blastocyst.
We have derived a cell line, RE1, from a pre-implantation rat blastocyst, resembling morphologically the L2 cell line from a parietal yolk sac carcinoma of the rat, as well as parietal endoderm cell lines of the mouse. The sub-cellular organization and epithelial characteristics of RE1 cells are described. The cells express cytokeratins of simple epithelia, and vimentin; and demonstrate synthesis of proteins of the extracellular matrix, such as laminin and collagen IV. Extensive Reichert's-like basement membrane is formed by RE1 cells when grown in suspension as aggregates. Cells have a microvillous surface morphology and abundant, rough endoplasmic reticulum which is swollen with apparent secretory material. These morphological and cytochemical features are characteristic of parietal endoderm cells in vivo, and the RE1 cell line is deduced to be rat parietal endoderm. In addition, RE1 cells were examined for expression of stage-specific embryonic antigens: cells reacted with antibody against SSEA-1/TEC-1 and EMA-1, constituting the first observation of parietal endoderm cells expressing the respective epitopes. RE1-cell monolayers did not generate transepithelial resistances or potential differences in vitro, consistent with their formation of leaky epithelia. Our observations on RE1-cell morphology and ultrastructure are consistent with the occurrence of epithelial-mesenchyme transitions in culture.
Notarianni
E
E
Comparative Biology Centre, The Medical School, University of Newcastle, Newcastle upon Tyne NE2 4HH, UK. elena.notarianni@ncl.ac.uk
Fléchon
J
J
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D001755:Blastocyst / Q000166:cytology* / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D004553:Electric Conductivity; D047108:Embryonic Development; D005314:Embryonic and Fetal Development; D004707:Endoderm / Q000166:cytology* / Q000378:metabolism; D004847:Epithelial Cells / Q000502:physiology / Q000648:ultrastructure; D005260:Female; D007150:Immunohistochemistry; D008855:Microscopy, Electron, Scanning; D008871:Microvilli / Q000648:ultrastructure; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar
D015415:Biomarkers