title: Molecular biology of acetyl-CoA metabolism.
abstract: We have characterized the expression of potential acetyl-CoA-generating genes (acetyl-CoA synthetase, pyruvate decarboxylase, acetaldehyde dehydrogenase, plastidic pyruvate dehydrogenase complex and ATP-citrate lyase), and compared these with the expression of acetyl-CoA-metabolizing genes (heteromeric and homomeric acetyl-CoA carboxylase). These comparisons have led to the development of testable hypotheses as to how distinct pools of acetyl-CoA are generated and metabolized. These hypotheses are being tested by combined biochemical, genetic and molecular biological experiments, which is providing insights into how acetyl-CoA metabolism is regulated.
authors.affiliation: Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA 50011, USA. dimmas@iastate.edu
authors.forename: B J
authors.initials: BJ
authors.lastname: Nikolau
authors.forename: D J
authors.initials: DJ
authors.lastname: Oliver
authors.forename: P S
authors.initials: PS
authors.lastname: Schnable
authors.forename: E S
authors.initials: ES
authors.lastname: Wurtele
mesh_terms: D001275:ATP Citrate (pro-S)-Lyase / Q000235:genetics; D000106:Acetate-CoA Ligase / Q000235:genetics; D000105:Acetyl Coenzyme A / Q000378:metabolism*; D000445:Aldehyde Oxidoreductases / Q000235:genetics; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics*; D008967:Molecular Biology / Q000379:methods; D018087:Plastids / Q000201:enzymology; D011767:Pyruvate Decarboxylase / Q000235:genetics; D011768:Pyruvate Dehydrogenase Complex / Q000235:genetics
chemical_list: D011768:Pyruvate Dehydrogenase Complex; D000105:Acetyl Coenzyme A; D000445:Aldehyde Oxidoreductases; C018699:aldehyde dehydrogenase (NAD(P)+); D001275:ATP Citrate (pro-S)-Lyase; D011767:Pyruvate Decarboxylase; D000106:Acetate-CoA Ligase