title: Restoration of vancomycin susceptibility in Enterococcus faecalis by antiresistance determinant gene transfer.
abstract: We assessed the ability of gene transfer to reverse vancomycin resistance in class A (VanA) glycopeptide-resistant Enterococcus faecalis. Recombinant shuttle vectors containing a vanH promoter-vanA antisense gene cassette fully restored vancomycin susceptibility through a combined transcriptional activator binding domain decoy and inducible vanA antisense RNA effect.
authors.affiliation: Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA.
authors.forename: C
authors.initials: C
authors.lastname: Torres Viera
authors.forename: S
authors.initials: S
authors.lastname: Tsiodras
authors.forename: H S
authors.initials: HS
authors.lastname: Gold
authors.forename: E P
authors.initials: EP
authors.lastname: Coakley
authors.forename: C
authors.initials: C
authors.lastname: Wennersten
authors.forename: G M
authors.initials: GM
authors.lastname: Eliopoulos
authors.forename: R C
authors.initials: RC
authors.lastname: Moellering
authors.forename: R T
authors.initials: RT
authors.lastname: Inouye
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors / Q000235:genetics; D019729:Carbon-Oxygen Ligases / Q000037:antagonists & inhibitors / Q000235:genetics; D004347:Drug Interactions; D013293:Enterococcus faecalis / Q000187:drug effects / Q000235:genetics*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000235:genetics / Q000494:pharmacology*; D014169:Transformation, Bacterial; D014640:Vancomycin / Q000494:pharmacology*; D020713:Vancomycin Resistance / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D016376:Oligonucleotides, Antisense; C071437:VanA ligase, Bacteria; D014640:Vancomycin; D019729:Carbon-Oxygen Ligases