Urinary F2-isoprostanes formation in kidney transplantation.
BACKGROUND
Oxygen free-radical mediated lipid peroxidation has been implicated in many diseases such as chronic renal failure, hemodialysis and chronic kidney transplant rejection. However, insight into the role of free radical generation in kidney transplantation has been constrained by the limitations of current indexes of oxidant stress in vivo. Isoprostaglandin F2alpha type-III (iPF2alpha-III, formerly known as 8-iso-prostaglandin F2alpha) is emerging as a reliable marker of oxidant stress in vivo. The purpose of our study was to investigate iPF2alpha-III formation as an index of lipid peroxidation in the 5 d following kidney transplantation.


METHODS
Urinary iPF2alpha-III measurements were performed by enzyme immunoassay from day I to 5 in 11 patients undergoing kidney transplantation. Results were compared with 11 healthy volunteers matched in sex, age and cigarette smoking.


RESULTS
Urinary excretion of iPF2alpha-III at day 1 did not significantly differ between control and transplant group (111 +/- 17 vs. 92 +/- 10 pM/ mM creatinine, respectively, NS). Urinary iPF2alpha-III levels did not differ between day 1 to 5, and were not correlated to cold ischaemia time.


CONCLUSIONS
Our study shows no evidence of enhanced lipid peroxidation in the first 5 d following kidney transplantation.
Cracowski
J L
JL
Laboratory of Pharmacology, Faculté de Médecine de Grenoble, La Tronche, France. Jean-Luc.Cracowski@ujf-grenoble.fr
Souvignet
C
C
Quirin
N
N
Grosbois
X
X
Bayle
F
F
Stanke-Labesque
F
F
Vialtel
P
P
Bessard
G
G
D000328:Adult; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D016030:Kidney Transplantation; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D011184:Postoperative Period; D011460:Prostaglandins F / Q000652:urine*; D012044:Regression Analysis
D011460:Prostaglandins F