title: [Detection of minimal residual disease in Ewing's sarcoma using RT-PCR].
abstract: BACKGROUND
  More than 90% of Ewing's sarcomas (ES) contain a fusion of the EWS and FLI-1 genes, due to the t(11;22)(q23;q12) translocation. At the molecular level, the EWS-FLI-1 rearrangement shows great diversity. Specifically, many different combinations of exons from EWS-FLI-1 encode in-frame fusion transcripts and result in differences in length and composition of the chimeric protein, which function as an oncogenic aberrant transcription factor. The finding of this translocation gives evidence for the presence of ES cells. The aim of this prospective study was to verify applicability of the RT-PCR method for the detection of minimal residual disease in patients with ES.
  
  
  RESULTS
  Conditions for the detection of Ewing's sarcoma cells by means of the reverse-transcriptase polymerase chain reaction (RT-PCR) at fusion transcripts in peripheral blood, bone marrow (BM) and autologous hematopoietic stem cell grafts in patients with ES were appointed. 31 samples of BM, 5 samples of blood and 7 peripheral blood grafts obtained from 23 patients were investigated. Presence of tumor cells was identified in 7 BM samples from 7 different patients (31 samples from 16 patients), all the peripheral blood and graft samples were negative.
  
  
  CONCLUSIONS
  The high sensitivity of RT-PCR method in detection of cells bearing t(11;22)(q23;q12) was demonstrated in the experimental model and clinical samples. Likewise the literary statements, the RT-PCR method was found to be more sensitive than cytology.
authors.affiliation: Klinika dĕtské onkologie 2. LF UK a FNM, Praha.
authors.forename: A
authors.initials: A
authors.lastname: Vícha
authors.forename: D
authors.initials: D
authors.lastname: Sumerauer
authors.forename: T
authors.initials: T
authors.lastname: Eckschlager
authors.forename: J
authors.initials: J
authors.lastname: Koutecký
authors.forename: P
authors.initials: P
authors.lastname: Kavan
authors.forename: V
authors.initials: V
authors.lastname: Smelhaus
authors.forename: J
authors.initials: J
authors.lastname: Stanková
authors.forename: P
authors.initials: P
authors.lastname: Gajdos
authors.forename: E
authors.initials: E
authors.lastname: Stejskalová
mesh_terms: D000293:Adolescent; D001859:Bone Neoplasms / Q000473:pathology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D008297:Male; D018365:Neoplasm, Residual; D012512:Sarcoma, Ewing / Q000175:diagnosis* / Q000235:genetics / Q000556:secondary*; D012680:Sensitivity and Specificity