[Study of experimental mixed bone marrow transplantation].
OBJECTIVE
To explore strategies for preventing graft versus host disease (GVHD) while preserving graft versus leukemia (GVL) effects in allogeneic bone marrow transplantation(BMT).


METHODS
Allogeneic bone marrow and spleen cells mixed with syngeneic spleen/bone marrow cells were transplanted into lethally irradiated inbred mice. The mortality of the recipient mice from GVHD, the number of CFU-S, the origin of the bone marrow cells responsible for the reconstitution at different time after transplantation and the proliferative reaction against the stimulating cells from the syngeneic or allogeneic mice were observed and compared with those in the conventional syngeneic and allogeneic BMT. L615 leukemia model was used to evaluate the GVL effect of mixed BMT.


RESULTS
Lethal GVHD was effectively prevented by the syngeneic spleen cells which showed no harmful effect on the CFU-S reconstitution. The medium survival time of the mixed transplanted L615 mice was significantly prolonged.


CONCLUSIONS
The grafts composed of allogeneic bone marrow and spleen cells mixed with syngeneic spleen cells, make the recipient mice have transient GVL effects resulted from the MHC mismatched immunocompetent cells and alleviated the severe GVHD of allogeneic BMT.
Luo
H
H
Department of Hematology and Oncology, Naval General Hospital, Beijing 100037.
Yang
P
P
He
F
F
D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods*; D017690:Cell Transplantation / Q000379:methods*; D005260:Female; D006086:Graft vs Host Disease / Q000517:prevention & control; D007938:Leukemia / Q000628:therapy*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology*; D014916:Whole-Body Irradiation