title: Mimotopes for tumor-specific T lymphocytes in human cancer determined with combinatorial peptide libraries.
abstract: Mimotopes of a tumor-associated T cell epitope were determined using randomized and combinatorial peptide libraries and a CD8(+) T cell clone specific for the cutaneous T cell lymphoma cell line MyLa. Antigen recognition by this clone was found to be HLA-B8 restricted. More than 80 % of HLA-matched patients with cutaneous T cell lymphoma had mimotope-specific CD8(+) T cells in their peripheral blood. Mimotope-specific T cells isolated and expanded from a patient lysed MyLa cells in in vitro assays thus demonstrating their cytolytic capacity and tumor specificity. Mimotope vaccination of a patient without detectable mimotope-specific T cells induced frequencies of these cells of up to 1.82 % of the peripheral blood CD8(+) T cells.
authors.affiliation: Department of Dermatology and Allergy, Charité, Humboldt University, Berlin, Germany. shvdburg@worldonline.nl
authors.forename: T
authors.initials: T
authors.lastname: Linnemann
authors.forename: S
authors.initials: S
authors.lastname: Tumenjargal
authors.forename: S
authors.initials: S
authors.lastname: Gellrich
authors.forename: K
authors.initials: K
authors.lastname: Wiesmüller
authors.forename: K
authors.initials: K
authors.lastname: Kaltoft
authors.forename: W
authors.initials: W
authors.lastname: Sterry
authors.forename: P
authors.initials: P
authors.lastname: Walden
mesh_terms: D000595:Amino Acid Sequence; D000951:Antigens, Neoplasm / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D020650:Combinatorial Chemistry Techniques; D018984:Epitopes, T-Lymphocyte; D015788:HLA-A1 Antigen / Q000235:genetics; D015795:HLA-B8 Antigen / Q000235:genetics; D006801:Humans; D016410:Lymphoma, T-Cell, Cutaneous / Q000276:immunology*; D008969:Molecular Sequence Data
chemical_list: D000951:Antigens, Neoplasm; D018984:Epitopes, T-Lymphocyte; D015788:HLA-A1 Antigen; D015795:HLA-B8 Antigen