Early commitment of adoptively transferred CD4+ T cells following particle-mediated DNA vaccination: implications for the study of immunomodulation.
The early responses of CD4+ T cells to particle-mediated DNA immunisation were investigated using OVA-specific TCR-transgenic CD4+ T cells. Following adoptive transfer of these cells, mice were immunised by delivery into the skin of a plasmid encoding ovalbumin. Transgenic T cells underwent a rapid and transient antigen-specific activation, followed by clonal expansion (up to approximately 6% of total lymphocytes). Immunisation with ovalbumin in CFA evoked similar responses with slightly faster kinetics. Numerous antigen-specific T cells synthesising IFN-gamma (Th1) and IL-4 (Th2) were detectable using both intracellular staining and ELISPOT assays. This study provides a quantitative analysis of both T cell proliferation and Th1/Th2 balance following particle-mediated DNA immunisation and establishes a robust and sensitive model in which to assess modulation of helper T cell responses in DNA vaccination.
Creusot
R J
RJ
Department of Immunology, Windeyer Institute of Medical Sciences, University College London, 46 Cleveland Street, W1P 6DB, London, UK.
Thomsen
L L
LL
van Wely
C A
CA
Topley
P
P
Tite
J P
JP
Chain
B M
BM
D019264:Adoptive Transfer; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000637:transplantation*; D002455:Cell Division; D002999:Clone Cells / Q000166:cytology / Q000276:immunology; D007371:Interferon-gamma / Q000032:analysis / Q000378:metabolism; D007376:Interleukin-2 / Q000096:biosynthesis / Q000276:immunology; D015847:Interleukin-4 / Q000032:analysis; D015848:Interleukin-5 / Q000032:analysis / Q000378:metabolism; D007700:Kinetics; D008198:Lymph Nodes / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D008863:Microspheres; D010047:Ovalbumin / Q000276:immunology; D015375:Receptors, Interleukin-2 / Q000276:immunology / Q000378:metabolism; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D014611:Vaccination; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology*
D015415:Biomarkers; D007376:Interleukin-2; D015848:Interleukin-5; D015375:Receptors, Interleukin-2; D019444:Vaccines, DNA; D015847:Interleukin-4; D007371:Interferon-gamma; D010047:Ovalbumin