title: NADP-malic enzyme from plants: a ubiquitous enzyme involved in different metabolic pathways.
abstract: NADP-malic enzyme (NADP-ME) is a widely distributed enzyme that catalyzes the oxidative decarboxylation of L-malate. Photosynthetic NADP-MEs are found in C4 bundle sheath chloroplasts and in the cytosol of CAM plants, while non-photosynthetic NADP-MEs are either plastidic or cytosolic in various plants. We propose a classification of plant NADP-MEs based on their physiological function and localization and we describe recent advances in the characterization of each isoform. Based on the alignment of amino acid sequences of plant NADP-MEs, we identify putative binding sites for the substrates and analyze the phylogenetic origin of each isoform, revealing several features of the molecular evolution of this ubiquitous enzyme.
authors.affiliation: Centro de Estudios Fotosintéticos y Bioquímicos, Universidad Nacional de Rosario, CONICET, Suipacha 531, 2000, Rosario, Argentina.
authors.forename: M F
authors.initials: MF
authors.lastname: Drincovich
authors.forename: P
authors.initials: P
authors.lastname: Casati
authors.forename: C S
authors.initials: CS
authors.lastname: Andreo
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D019143:Evolution, Molecular; D007700:Kinetics; D008291:Malate Dehydrogenase / Q000737:chemistry* / Q000145:classification; D008969:Molecular Sequence Data; D010802:Phylogeny; D010944:Plants / Q000201:enzymology*; D020033:Protein Isoforms
chemical_list: D020033:Protein Isoforms; D008291:Malate Dehydrogenase; C031917:malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)