title: [Development of an alternative method to the monkey neurovirulence test for oral poliovirus vaccines]
abstract: Each oral poliovirus vaccine lot contains attenuated and small amounts of wildtype viruses. Vaccines containing more than a certain limit of wildtype viruses may cause a vaccine associated poliomyelitis. To provide safe vaccines for humans, each newly manufactured vaccine lot is tested in the monkey neurovirulence test. A certain point mutation on the poliovirus genome has been shown to be responsible for the attenuated phenotype of the vaccine virus. We developed a quantitative PCR method to determine the wildtype proportion at position 472 of poliovirus type 3 genome. This method possibly can be used as an alternative for the monkey neurovirulence test.
authors.affiliation: Paul-Ehrlich-Institut, D-Langen.
authors.forename: Arthur
authors.initials: A
authors.lastname: Kaul
authors.forename: Micha
authors.initials: M
authors.lastname: Nübling
authors.forename: Martina
authors.initials: M
authors.lastname: Kempfer
authors.forename: Ivo
authors.lastname: Schmidt
authors.forename: Johannes
authors.initials: J
authors.lastname: Löwer