title: Typing of Acinetobacter baumannii isolated from hospital-acquired respiratory infections in a tertiary care centre in southern India.
abstract: In an attempt to define the epidemiology of Acinetobacter baumannii infection, 27 isolates, obtained from hospital-acquired respiratory infections, were typed using random amplified polymorphic DNA (RAPD) profile and antimicrobial susceptibility patterns. Ten different patterns were obtained with ERIC2 primer: 14 isolates had a similar profile representing a single strain. Within RAPD types, isolates could be further classified based on their antibiogram; however, strains of different types had similar antibiograms. This study showed that many different genetic types of A. baumannii are prevalent in our hospital. While antibiograms alone are not sufficiently discriminatory, RAPD typing helps in identifying outbreaks and in assessing infection control procedures within a hospital.
authors.affiliation: Department of Clinical Microbiology, Christian Medical College and Hospital, Vellore, Tamil Nadu, 632004, India.
authors.forename: E
authors.initials: E
authors.lastname: Mathai
authors.forename: M E
authors.initials: ME
authors.lastname: Kaufmann
authors.forename: V S
authors.initials: VS
authors.lastname: Richard
authors.forename: G
authors.initials: G
authors.lastname: John
authors.forename: K N
authors.initials: KN
authors.lastname: Brahmadathan
mesh_terms: D000046:Academic Medical Centers; D000150:Acinetobacter / Q000145:classification* / Q000235:genetics*; D000151:Acinetobacter Infections / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology* / Q000517:prevention & control; D003428:Cross Infection / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology* / Q000517:prevention & control; D016172:DNA Fingerprinting / Q000379:methods; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004196:Disease Outbreaks / Q000517:prevention & control / Q000706:statistics & numerical data*; D004352:Drug Resistance, Microbial; D006801:Humans; D007194:India / Q000453:epidemiology; D017053:Infection Control / Q000379:methods; D008826:Microbial Sensitivity Tests; D019105:Random Amplified Polymorphic DNA Technique; D012141:Respiratory Tract Infections / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology* / Q000517:prevention & control; D012703:Serotyping / Q000379:methods
chemical_list: D004269:DNA, Bacterial