title: A major conformational change in p97 AAA ATPase upon ATP binding.
abstract: AAA ATPases play central roles in cellular activities. The ATPase p97, a prototype of this superfamily, participates in organelle membrane fusion. Cryoelectron microscopy and single-particle analysis revealed that a major conformational change of p97 during the ATPase cycle occurred upon nucleotide binding and not during hydrolysis as previously hypothesized. Furthermore, our study indicates that six p47 adaptor molecules bind to the periphery of the ring-shaped p97 hexamer. Taken together, these results provide a revised model of how this and possibly other AAA ATPases can translate nucleotide binding into conformational changes of associated binding partners.
authors.affiliation: Department of Cell Biology, Scripps Research Institute, La Jolla, CA 92037, USA.
authors.forename: I
authors.initials: I
authors.lastname: Rouiller
authors.forename: V M
authors.initials: VM
authors.lastname: Butel
authors.forename: M
authors.initials: M
authors.lastname: Latterich
authors.forename: R A
authors.initials: RA
authors.lastname: Milligan
authors.forename: E M
authors.initials: EM
authors.lastname: Wilson-Kubalek
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000737:chemistry* / Q000378:metabolism* / Q000648:ultrastructure; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000266:Adenylyl Imidodiphosphate / Q000378:metabolism; D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry / Q000648:ultrastructure; D002417:Cattle; D020285:Cryoelectron Microscopy; D007091:Image Processing, Computer-Assisted; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008958:Models, Molecular; D052066:N-Ethylmaleimide-Sensitive Proteins; D009687:Nuclear Proteins / Q000737:chemistry* / Q000378:metabolism* / Q000648:ultrastructure; D011485:Protein Binding; D011487:Protein Conformation; D050600:SNARE Proteins; D033921:Vesicular Transport Proteins
chemical_list: D002352:Carrier Proteins; D008565:Membrane Proteins; D009687:Nuclear Proteins; D050600:SNARE Proteins; D033921:Vesicular Transport Proteins; D000266:Adenylyl Imidodiphosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; C404942:p97 ATPase; D052066:N-Ethylmaleimide-Sensitive Proteins; C498733:Nsf protein, mouse