Polarity of human parainfluenza virus type 3 infection in polarized human lung epithelial A549 cells: role of microfilament and microtubule.
Human parainfluenza virus type 3 (HPIV-3) is an airborne pathogen that infects the epithelial cells of the respiratory tract. In the present study we investigated the interaction of HPIV-3 with the type II alveolar human lung polarized epithelial A549 cells. Although HPIV-3 entry and budding were bidirectional from both the apical and the basolateral domains, HPIV-3 exhibited preferential entry and release from the apical pole. While disruption of the cellular actin microfilament and microtubule by cytochalasin D and nocodazole, respectively, had no effect on virus entry, disruption of the microtubule but not the microfilament inhibited HPIV-3 release.
Bose
S
S
Department of Virology, Lerner Research Institute, The Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA.
Malur
A
A
Banerjee
A K
AK
D008841:Actin Cytoskeleton / Q000502:physiology*; D016764:Cell Polarity; D004847:Epithelial Cells / Q000821:virology*; D006801:Humans; D008870:Microtubules / Q000502:physiology*; D010224:Parainfluenza Virus 3, Human / Q000472:pathogenicity* / Q000502:physiology; D011650:Pulmonary Alveoli / Q000166:cytology*; D014407:Tumor Cells, Cultured