Gene expression of angiogenesis related factors in glioma.
Angiogenesis plays an important role in growth and proliferation of cancer. Various angiogenic and angiostatic factors regulate angiogenesis. In this study, we examined gene expression of the angiopoietin family including angiopoietin 1 (Ang1) and angiopoietin 2 (Ang2) in 39 gliomas and 5 glioma-xenografts by RT-PCR. Ang1 and Ang2 genes were expressed in 54%, and 77% of gliomas, respectively. The expression of Ang1 was significantly correlated with the expression of Ang2. Both Ang1 and Ang2 were shown to be expressed in the glioma cells. Ang2 gene expression was correlated with VEGF gene expression. Angiopoietin molecules may synergistically cooperate in growth and vascularization in glioma.
Osada
H
H
Department of Pathology, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259-1193, Japan.
Tokunaga
T
T
Hatanaka
H
H
Kawakami
T
T
Tsuchida
T
T
Abe
Y
Y
Tsugu
A
A
Kijima
H
H
Yamazaki
H
H
Shima
K
K
Osamura
Y
Y
Ueyama
Y
Y
Nakamura
M
M
D042683:Angiopoietin-1; D042702:Angiopoietin-2; D000818:Animals; D016009:Chi-Square Distribution; D016228:Endothelial Growth Factors / Q000378:metabolism; D015870:Gene Expression / Q000502:physiology*; D005909:Glioblastoma / Q000098:blood supply / Q000378:metabolism; D005910:Glioma / Q000098:blood supply / Q000378:metabolism*; D006801:Humans; D008222:Lymphokines / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism*; D051379:Mice; D009363:Neoplasm Proteins / Q000378:metabolism*; D009389:Neovascularization, Pathologic / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D011518:Proto-Oncogene Proteins; D014183:Transplantation, Heterologous; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D042683:Angiopoietin-1; D042702:Angiopoietin-2; C467628:Angpt1 protein, mouse; D016228:Endothelial Growth Factors; D008222:Lymphokines; C105373:MEN1 protein, human; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins; D011506:Proteins; D011518:Proto-Oncogene Proteins; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors