Regulation of interleukin 1 beta RNA expression in the common carp, Cyprinus carpio L.
The intron-exon organisation of the carp IL-1beta gene consists of 2455bp and comprises seven exons. Three IL-1beta RNA transcripts have been found in carp: (1) a fully spliced product; (2) exon 1-7 with introns 5 and 6; and (3) exon 1-7 with intron 5 only. The intron-containing products probably represent partially spliced transcripts. IL-1beta mRNA expression in carp was semi-quantitatively analysed by RT-PCR in multiple organs, including brain and pituitary. Constitutive expression of the IL-1beta mRNA was found in these organs with a predominant expression in the immune organs head kidney and spleen. Furthermore, a scattered distribution of IL-1beta producing cells was shown by in situ hybridisations of head kidney tissue. Administration of phorbol-myristate-acetate (PMA), lipopolysaccharide (LPS) or retinoic acid (RA), to phagocytes isolated from the head kidney, resulted in expression of IL-1beta intron-containing transcripts. Of these, only PMA and LPS were stimulators that induced the fully spliced transcript. A role for the nuclear factor (NF)-kappaB pathway in carp IL-1beta expression was shown with suppression of the LPS-induced IL-1beta expression by NF-kappaB inhibitor pyrrolidine dithiocarbamate (PDTC). Cortisol was able to inhibit in vitro constitutive expression of IL-1beta transcripts. Addition of cortisol simultaneously with LPS could not substantially inhibit transcription.
Engelsma
M Y
MY
Cell Biology and Immunology Group, Wageningen Institute of Animal Sciences, Wageningen University, PO Box 338, 6700 AH, Wageningen, The Netherlands.
Stet
R J
RJ
Schipper
H
H
Verburg-van Kemenade
B M
BM
D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D005091:Exons; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D017403:In Situ Hybridization; D007375:Interleukin-1 / Q000235:genetics*; D007438:Introns; D007668:Kidney / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014018:Tissue Distribution
D017931:DNA Primers; D007375:Interleukin-1; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D013755:Tetradecanoylphorbol Acetate