The rat H3 receptor: gene organization and multiple isoforms.
Genomic DNA analysis revealed that the coding region of the rat histamine H3 receptor comprises three exons interrupted by two introns of approximately 1 kb each. Several H3 receptor mRNA variants were identified by PCR and cDNA cloning and sequencing. Four variants generated by pseudo-intron retention/deletion at the level of the third intracellular loop were designated H3(445), H3(413), H3(410), and H3(397), according to the length of their deduced amino acid sequence and display differential tissue expression. When expressed in CHO-K1 or Cos-1 cells, the H3(445), H3(413), and H3(397) were found to generate specific 125I iodoproxyfan binding of similar pharmacological profile. In addition, we identified two short variants, termed H3(nf1) and H3(nf2), which correspond to frame shift and stop codon interposition, respectively, and are presumably nonfunctional, among which H3(nf2) displays brain expression similar to that of the longer isoforms.
Morisset
S
S
Unité de Neurobiologie et Pharmacologie Moléculaire (U109) de l'INSERM, Centre Paul Broca, 2 ter rue d'Alésia, Paris, 75014, France.
Sasse
A
A
Gbahou
F
F
Héron
A
A
Ligneau
X
X
Tardivel-Lacombe
J
J
Schwartz
J C
JC
Arrang
J M
JM
D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000378:metabolism*; D016466:CHO Cells; D002460:Cell Line; D003001:Cloning, Molecular; D006224:Cricetinae; D016368:Frameshift Mutation; D014644:Genetic Variation; D017403:In Situ Hybridization; D007438:Introns; D007457:Iodine Radioisotopes; D009928:Organ Specificity; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D011869:Radioligand Assay; D051381:Rats; D018100:Receptors, Histamine H3 / Q000235:genetics* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D017384:Sequence Deletion; D014158:Transcription, Genetic; D014162:Transfection
D007457:Iodine Radioisotopes; D020033:Protein Isoforms; D018100:Receptors, Histamine H3; D011994:Recombinant Proteins