Isolation of two putative acyl-acyl carrier protein desaturase enzymes from Kochia scoparia.
5-Hexadecenoic acid can be used to produce a semichemical method to control the disease-carrying mosquito Culex quinquefasciatus. This unusual fatty acid is produced in the seed of Kochia scoparia. We have isolated two acyl-acyl carrier protein desaturases from this species and expressed them in Escherichia coli to facilitate functional characterisation.
Whitney
H
H
IACR-Long Ashton Research Station, University of Bristol, Long Ashton, Bristol BS41 9AF, UK. heather.whitney@bbsrc.ac.uk
Sayanova
O
O
Lewis
M J
MJ
Pickett
J
J
Napier
J A
JA
D000818:Animals; D003001:Cloning, Molecular; D003465:Culex; D004926:Escherichia coli; D005227:Fatty Acids / Q000378:metabolism*; D005784:Gene Amplification; D007527:Isoenzymes / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D019684:Magnoliopsida / Q000201:enzymology* / Q000235:genetics; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D010169:Palmitic Acids / Q000378:metabolism*; D010572:Pest Control, Biological; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D012639:Seeds / Q000201:enzymology
D005227:Fatty Acids; D007527:Isoenzymes; D010169:Palmitic Acids; D011994:Recombinant Proteins; C093821:hexadecenoic acid; D006899:Mixed Function Oxygenases; C018747:acyl-(acyl-carrier-protein)desaturase