Growth factors and cytokines modulate gene expression of cell-surface proteoglycans in human periodontal ligament cells.
Cell-surface proteoglycans have been known to be involved in many functions including interactions with components of the extracellular microenvironment, and act as co-receptors which bind and modify the action of various growth factors and cytokines. The purpose of this study was to determine the regulation by growth factors and cytokines on cell-surface proteoglycan gene expression in cultured human periodontal ligament (PDL) cells. Subconfluent, quiescent PDL cells were treated with various concentrations of serum, bFGF, PDGF-BB, TGF-beta1, IL-beta1, and IFN-gamma. RT-PCR technique was used, complemented with Northern blot for syndecan-1, to examine the effects of these agents on the mRNA expression of five cell-surface proteoglycans (syndecan-1, syndecan-2, syndecan-4, glypican and betaglycan). Syndecan-1 mRNA levels increased in response to serum, bFGF and PDGF-BB, but decreased in response to TGF-beta1, IL-1beta and IFN-gamma. In contrast, syndecan-2 mRNA levels were upregulated by TGF-beta1 and IL-1beta stimulation, but remained unchanged with the other agents. Betaglycan gene expression decreased in response to serum, but was upregulated by TGF-beta1 and unchanged by the other stimulants. Additionally, syndecan-4 and glypican were not significantly altered in response to the regulator molecules studied, with the exception that glypican is decreased in response to IFN-gamma. These data demonstrate that the gene expression of the five cell-surface proteoglycans studied is differentially regulated in PDL cells lending support to the notion of distinct functions for these cell-surface proteoglycans.
Worapamorn
W
W
Connective Tissue Research Laboratory, School of Dentistry, The University of Queensland, Turbot Street, Brisbane, Qld 4000, Australia.
Haase
H R
HR
Li
H
H
Bartold
P M
PM
D000077214:Becaplermin; D002455:Cell Division / Q000187:drug effects; D002462:Cell Membrane / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000494:pharmacology*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D006133:Growth Substances / Q000494:pharmacology*; D019812:Heparan Sulfate Proteoglycans / Q000235:genetics; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology; D008562:Membrane Glycoproteins / Q000235:genetics; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D011509:Proteoglycans / Q000235:genetics*; D020574:Proto-Oncogene Proteins c-sis; D018125:Receptors, Transforming Growth Factor beta / Q000235:genetics; D011994:Recombinant Proteins / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D053668:Syndecan-1; D053669:Syndecan-2; D053671:Syndecan-4; D053667:Syndecans; D014158:Transcription, Genetic; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D001613:beta 2-Microglobulin / Q000235:genetics
D016207:Cytokines; D006133:Growth Substances; D019812:Heparan Sulfate Proteoglycans; D007375:Interleukin-1; D008562:Membrane Glycoproteins; D010982:Platelet-Derived Growth Factor; D011509:Proteoglycans; D020574:Proto-Oncogene Proteins c-sis; D018125:Receptors, Transforming Growth Factor beta; D011994:Recombinant Proteins; C508241:SDC1 protein, human; C508245:SDC2 protein, human; C508304:SDC4 protein, human; D053668:Syndecan-1; D053671:Syndecan-4; D053667:Syndecans; D016212:Transforming Growth Factor beta; D001613:beta 2-Microglobulin; D016222:Fibroblast Growth Factor 2; C071736:betaglycan; D053669:Syndecan-2; D000077214:Becaplermin; D007371:Interferon-gamma