Effects of nicotinamide coenzymes on the stability of enzyme activities and proteins in niacin-deficient quail tissues against trypsin treatment.
The stability of liver and muscle enzymes and proteins in niacin-deficient quail towards trypsin treatment in the presence and absence of coenzymes, NAD or NADP, was characterized. The protection of liver dehydrogenases by coenzymes was low when they are subjected to trypsin digestion for 60 min. In contrast, in the muscle there was substantial protection against trypsin inactivation of glyceraldehyde-3-phosphate dehydrogenase by NAD and of 6-phosphogluconate dehydrogenase by NADP. Among all enzymes tested, glyceraldehyde-3-phosphate dehydrogenase showed the greatest protection against trypsin inactivation by NAD. SDS-polyacrylamide gel electrophoresis demonstrated that muscle proteins from the niacin-deficient group were more substantially protected compared to control and pair-fed groups when liver and muscle extracts were spiked with NAD and subjected to trypsin digestion. Overall results suggest that niacin deficiency exerted specific destabilizing effects on the stability of enzymes and proteins in muscle.
Park
I K
IK
Department of Biology, Faculty of Science, Dongguk University, 100-715, Seoul, South Korea.ikpark@dgu.ac.kr
Koh
Y H
YH
D000818:Animals; D003067:Coenzymes / Q000494:pharmacology*; D003370:Coturnix; D004591:Electrophoresis, Polyacrylamide Gel; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D009132:Muscles / Q000201:enzymology; D009243:NAD / Q000378:metabolism / Q000494:pharmacology; D009249:NADP / Q000378:metabolism / Q000494:pharmacology; D009525:Niacin / Q000172:deficiency*; D009536:Niacinamide / Q000494:pharmacology*; D010088:Oxidoreductases / Q000378:metabolism; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism; D013997:Time Factors; D014357:Trypsin / Q000494:pharmacology*
D003067:Coenzymes; D009243:NAD; D009536:Niacinamide; D009525:Niacin; D009249:NADP; D010088:Oxidoreductases; D010734:Phosphogluconate Dehydrogenase; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D014357:Trypsin