Sequence analysis, gene expression, and chromosomal assignment of mouse Borg4 gene and its human orthologue.
The Borg (binder of Rho GTPases) family proteins interact with CDC42 and TC10 in a guanosine triphosphate (GTP)-dependent manner. We have isolated a full-length cDNA of the mouse Borg4 gene, which is a member of this family. Sequence analysis revealed that this gene encoded a putative 349-amino acid protein. By reverse transcription--coupled polymerase chain reaction (RT-PCR) analysis, we observed that Borg4 was expressed ubiquitously in adult tissues. Additionally, we determined the entire cDNA sequence of the putative human Borg4 orthologue. By fluorescence in situ hybridization, mouse Borg4 and the putative human orthologue have been assigned to mouse chromosome 11E and human chromosome 17q24-25, which has been described as syntenic to the mouse region.
Osada
N
N
Division of Genetic Resources, National Institute of Infectious Diseases, Tokyo, Japan. osada@nih.go.jp
Kusuda
J
J
Suzuki
Y
Y
Sugano
S
S
Hashimoto
K
K
D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D001798:Blood Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002352:Carrier Proteins; D002874:Chromosome Mapping; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics*; D003598:Cytoskeletal Proteins; D020549:GTP Phosphohydrolase Activators; D020558:GTP Phosphohydrolases; D020555:GTP-Binding Protein Regulators; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D051379:Mice; D008969:Molecular Sequence Data; D016601:RNA-Binding Proteins; D017422:Sequence Analysis, DNA; D020764:cdc42 GTP-Binding Protein; D020741:rho GTP-Binding Proteins
D048868:Adaptor Proteins, Signal Transducing; D001798:Blood Proteins; C422984:CDC42EP2 protein, human; C484093:CDC42EP3 protein, human; C422985:CDC42EP4 protein, human; C484094:CDC42EP5 protein, human; D002352:Carrier Proteins; C484092:Cdc42ep5 protein, mouse; D003598:Cytoskeletal Proteins; D020549:GTP Phosphohydrolase Activators; D020555:GTP-Binding Protein Regulators; D016601:RNA-Binding Proteins; C075598:CDC42EP1 protein, human; D020558:GTP Phosphohydrolases; D020764:cdc42 GTP-Binding Protein; D020741:rho GTP-Binding Proteins