title: Megakaryocytic differentiation is accompanied by a reduction in cell migratory potential.
abstract: Megakaryocytes (MKs) have been found in the peripheral circulation, suggesting that they can migrate out of the bone marrow. In order to evaluate if megakaryocytic differentiation confers a migratory phenotype, we investigated this property in the haematopoietic cell lines MO7e and UT-7/mpl and in CD34+ progenitor cells before and after induction of differentiation by thrombopoietin (TPO). Migration was studied using a bicompartmental culture system in the presence or absence of a bone marrow endothelial cell monolayer. Preincubation with TPO led to a significant reduction in stromal cell-derived factor-1 (SDF-1)-induced migration of MO7e cells (0.7% +/- 0.08% for TPO-treated vs. 2.6% +/- 0.3% for controls P < 0.05). A similar decreased migratory response was seen with UT-7/mpl cells (7.4% +/- 0.4% for TPO-treated vs. 11.1% +/- 0.01% for controls, P<0.05), although these cells did not migrate in response to SDF-1. CD34+ cells partially differentiated with TPO showed decreased migration following further TPO-induced maturation (13.9% +/- 1.8% for TPO-treated vs. 24.1% +/- 1.8% for untreated, P < 0.05). This reduction was more pronounced in the large MK (> or = 4n) fraction. These results demonstrate that megakaryocytic differentiation is accompanied by a partial suppression of the haematopoietic cell migratory phenotype.
authors.affiliation: Cell Biology Group, Centre for Cardiovascular Biology and Medicine, Department of Medicine, University College London, The Rayne Institute, London, UK.
authors.forename: A
authors.initials: A
authors.lastname: Mathur
authors.forename: Y
authors.initials: Y
authors.lastname: Hong
authors.forename: J
authors.initials: J
authors.lastname: Martin
authors.forename: J
authors.initials: J
authors.lastname: Erusalimsky
mesh_terms: D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002460:Cell Line; D002465:Cell Movement; D002478:Cells, Cultured; D018920:Coculture Techniques; D005434:Flow Cytometry; D006410:Hematopoiesis; D006801:Humans; D008533:Megakaryocytes / Q000166:cytology*; D013926:Thrombopoietin / Q000494:pharmacology
chemical_list: D013926:Thrombopoietin