title: Release of WBC-derived IL-1 receptor antagonist into supernatants of RBCs: influence of storage time and filtration.
abstract: BACKGROUND
  Transfusion-associated immunodepression may be related to the transfer of immunoinhibitory cytokines with blood components.
  
  
  METHODS
  After evidence of increasing concentrations of IL-1 receptor antagonist (IL-1RA) but not of IL-10 was obtained in supernatants of stored RBC units that were WBC-reduced by centrifugation (C-RBCs) in a pilot study, IL-1RA concentrations were determined weekly in supernatants of C-RBCs and in units that underwent prestorage WBC reduction by in-line filtration (F-RBCs) over a 49-day storage. For assessing total IL-1RA content, complete cell lysis by repeated freezing and thawing was done. The results were related to the changes in WBC count during storage. The dependency of IL-1RA content on preparation procedures was assessed.
  
  
  RESULTS
  The prestorage IL-1RA concentration in C-RBCs (859 +/- 218 pg/mL) was significantly higher than in F-RBC (75 +/- 13 pg/mL). Whereas no changes were seen in F-RBCs during storage, IL-1RA levels in C-RBC supernatants drastically increased to levels about 50 times those in normal plasma (16,327 +/- 2,686 pg/mL on Day 49). Follow-up analysis revealed stringent correlation between IL-1RA release into supernatants and the current loss of WBCs (r = 0.79, n = 42; p<0.001). The total IL-1RA content did not change during storage and was directly dependent on prestorage WBC count. Preparation procedures altered the IL-1RA content only by WBC reduction.
  
  
  CONCLUSIONS
  The immunosuppressive cytokine IL-1RA is transmitted by RBCs in relation to WBC content and storage time.
authors.affiliation: Institutes of Transfusion Medicine and Medical Immunology, Medical Faculty, Charité University Clinic, Humboldt University of Berlin, D-10098 Berlin, Germany.
authors.forename: I
authors.initials: I
authors.lastname: Büttnerova
authors.forename: H
authors.initials: H
authors.lastname: Bäumler
authors.forename: F
authors.initials: F
authors.lastname: Kern
authors.forename: H
authors.initials: H
authors.lastname: Radtke
authors.forename: H D
authors.initials: HD
authors.lastname: Volk
authors.forename: H
authors.initials: H
authors.lastname: Kiesewetter
authors.forename: W D
authors.initials: WD
authors.lastname: Döcke
mesh_terms: D001793:Blood Preservation; D002498:Centrifugation; D004912:Erythrocytes / Q000378:metabolism*; D006440:Hemofiltration; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein; D007958:Leukocyte Count; D007962:Leukocytes / Q000378:metabolism*; D009994:Osmolar Concentration; D010865:Pilot Projects; D011446:Prospective Studies; D012016:Reference Values; D012795:Sialoglycoproteins / Q000378:metabolism*; D013997:Time Factors
chemical_list: C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D012795:Sialoglycoproteins