A comparison of the effects of DNA-damaging agents and biotic elicitors on the induction of plant defense genes, nuclear distortion, and cell death.
Pea (Pisum sativum L. cv Alcan) endocarp tissue challenged with an incompatible fungal pathogen, Fusarium solani f. sp. phaseoli or fungal elicitors results in the induction of pathogenesis-related (PR) genes and the accumulation of pisatin, a phytoalexin. Essentially the same response occurs in pea tissue exposed to DNA-specific agents that crosslink or intercalate DNA. In this study, the effects of DNA-damaging agents were assessed relative to the inducible expression of several pea PR genes: phenylalanine ammonia lyase, chalcone synthase, and DRR206. Mitomycin C and actinomycin D mimicked the biotic elicitors in enhancing the expression of all three PR genes. The activities of these PR gene promoters, isolated from different plants, were evaluated heterologously in transgenic tobacco. It is remarkable that beta-glucuronidase expression was induced when plants containing the heterologous phenylalanine ammonia lyase, chalcone synthase, and DRR206 promoter-beta-glucuronidase chimeric reporter genes were treated by DNA-damaging agents. Finally, cytological analyses indicated that many of these agents caused nuclear distortion and collapse of the treated pea cells. Yet we observed that cell death is not necessary for the induction of the PR gene promoters assessed in this study. Based on these observations and previously published results, we propose that DNA damage or the associated alteration of chromatin can signal the transcriptional activation of plant defense genes.
Choi
J J
JJ
Department of Plant Pathology, Washington State University, Pullman, Washington 99164-6430, USA.
Klosterman
S J
SJ
Hadwiger
L A
LA
D016923:Cell Death; D002467:Cell Nucleus / Q000648:ultrastructure*; D002686:Chitin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D048271:Chitosan; D002945:Cisplatin / Q000494:pharmacology; D004249:DNA Damage; D003609:Dactinomycin / Q000494:pharmacology*; D004742:Enhancer Elements, Genetic / Q000235:genetics; D008856:Microscopy, Fluorescence; D016685:Mitomycin / Q000494:pharmacology; D018532:Pisum sativum / Q000187:drug effects / Q000235:genetics* / Q000648:ultrastructure; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000235:genetics / Q000648:ultrastructure; D011401:Promoter Regions, Genetic / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics / Q000302:isolation & purification; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics / Q000648:ultrastructure
D018749:RNA, Plant; D002686:Chitin; D003609:Dactinomycin; D016685:Mitomycin; D048271:Chitosan; D002945:Cisplatin