title: Localization of the Drosophila MAGUK protein Polychaetoid is controlled by alternative splicing.
abstract: The Drosophila membrane-associated guanylate kinase (MAGUK) protein Polychaetoid (Pyd) is required for dorsal closure of the embryo, sensory organ patterning, and cell fate specification in the developing eye. We demonstrate that pyd is alternatively spliced resulting in two isoforms that differ by the presence or absence of exon 6. To determine the role of alternative splicing in Pyd function, we generated antibodies specific for each isoform. We find that the exon 6(+) form of Pyd is localized at adherens junctions of embryonic and imaginal epithelia, while the exon 6(-) form is distributed broadly along the lateral membrane. These results suggest that localization of Pyd is controlled by alternative splicing and raise the possibility that exon 6 represents a distinct protein-protein interaction domain.
authors.affiliation: Department of Biology, Emory University, Atlanta, GA 30322, USA.
authors.forename: X
authors.initials: X
authors.lastname: Wei
authors.forename: H M
authors.initials: HM
authors.lastname: Ellis
mesh_terms: D022005:Adherens Junctions / Q000378:metabolism; D000483:Alleles; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000378:metabolism; D015153:Blotting, Western; D019070:Cell Lineage; D002462:Cell Membrane / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D004330:Drosophila; D029721:Drosophila Proteins; D005091:Exons; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D051528:Guanylate Kinases; D006358:Hot Temperature; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009703:Nucleoside-Phosphate Kinase / Q000737:chemistry*; D010641:Phenotype; D010750:Phosphoproteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D011485:Protein Binding; D020033:Protein Isoforms; D017434:Protein Structure, Tertiary; D012313:RNA / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D062725:Tight Junction Proteins; D013997:Time Factors; D019076:Transgenes; D062826:Zonula Occludens-1 Protein
chemical_list: D000906:Antibodies; D018076:DNA, Complementary; D029721:Drosophila Proteins; D008565:Membrane Proteins; D010750:Phosphoproteins; D020033:Protein Isoforms; D062725:Tight Junction Proteins; D062826:Zonula Occludens-1 Protein; C569041:pyd protein, Drosophila; D012313:RNA; D009703:Nucleoside-Phosphate Kinase; D051528:Guanylate Kinases