title: Use of arbitrarily primed polymerase chain reaction to investigate Mycoplasma bovis outbreaks.
abstract: Colony lineages from three Mycoplasma bovis outbreaks representing different husbandry conditions in the United States were characterized with arbitrarily primed polymerase chain reaction (AP-PCR). Cases studied included a closed beef herd, a dairy calf ranch, and a feedlot. The DNA was obtained from colony lineages and used for AP-PCR with primers REP1R-I and REP2-I. Case A and C lineages were uniform by AP-PCR analysis. Lineages from case B showed heterogeneity with AP-PCR. Outbreaks A and C were therefore both infected by one source, while the ranch (case B) was infected by multiple calf shipments. The AP-PCR typing method provides genotypic epidemiological information to successfully characterize M. bovis from sequential sampling of outbreaks and different husbandry conditions.
authors.affiliation: Veterinary Medical Research Institute, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011, USA.
authors.forename: J A
authors.initials: JA
authors.lastname: Butler
authors.forename: C C
authors.initials: CC
authors.lastname: Pinnow
authors.forename: J U
authors.initials: JU
authors.lastname: Thomson
authors.forename: S
authors.initials: S
authors.lastname: Levisohn
authors.forename: R F
authors.initials: RF
authors.lastname: Rosenbusch
mesh_terms: D000822:Animal Husbandry; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000382:microbiology*; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification; D004196:Disease Outbreaks; D005260:Female; D008297:Male; D009174:Mycoplasma / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D011019:Pneumonia, Mycoplasma / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D014481:United States / Q000453:epidemiology
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial