Preparation and inhibitory activity on hyaluronidase of fully O-sulfated hyaluro-oligosaccharides.
Hyaluronan was partially depolymerized on a large-scale quantity using bacterial hyaluronidase (E.C. 4.2.2.1) for preparation of chemically fully O-sulfated oligosaccharides. The hyaluro-oligosaccharide (HAoligo) mixture obtained by partial digestion was repeatedly applied to low pressure gel permeation chromatographic separation to purify the size-unified oligosaccharide ranged from 4- to 20-mer. The purity and size of each HAoligo was confirmed by using proton nuclear magnetic resonance ((1)H NMR) spectroscopy, capillary electrophoresis (CE) on normal polarity mode, and a newly established separation method by normal phase chromatography with Amide-80 column. The purified HAoligos ranged 4- to 20-mer were applied to chemically fully O-sulfation. Characterization of chemically fully O-sulfated HAoligos was performed by both chemical compositional analyses after hydrolysis and (1)H NMR spectroscopy. While the anti-factor IIa activity of 4- to 20-mer O-sulfated HAoligos was less than 3.1 units/mg, the inhibitory action for hyaluronidase (bovine testicular hyaluronidase (E.C.3.2.1.35)) of the oligosaccharides ranged 16- to 20-mer were corresponding to 79% of that shown by fully O-sulfated hyaluronan (MW 100 kDa) through both competitive and noncompetitive effects.
Suzuki
A
A
Faculty of Pharmaceutical Sciences, Chiba University, 1-33, Yayoi, Inage, Chiba 263-8522, Japan.
Toyoda
H
H
Toida
T
T
Imanari
T
T
D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006820:Hyaluronic Acid / Q000737:chemistry*; D006821:Hyaluronoglucosaminidase / Q000037:antagonists & inhibitors*; D009844:Oligosaccharides / Q000737:chemistry / Q000494:pharmacology*; D013464:Sulfuric Acids / Q000737:chemistry
D004791:Enzyme Inhibitors; D009844:Oligosaccharides; D013464:Sulfuric Acids; D006820:Hyaluronic Acid; D006821:Hyaluronoglucosaminidase