Dextran, gelatin, and hydroxyethyl starch do not affect permeability for albumin in cat skeletal muscle.
OBJECTIVE
To evaluate the effects of the three commercially available colloid solutions, 6% dextran 70, 6% hydroxyethyl starch (HES) 200/0.5, and 3.5% urea-linked gelatin on permeability for human albumin in a skeletal muscle in vivo model by evaluating their effects on the reflection coefficient for albumin.


METHODS
Controlled laboratory study.


METHODS
University research laboratory.


METHODS
Eighteen adult cats.


METHODS
The autoperfused and denervated calf muscles of the cat hindlimb were placed in a plethysmograph. The transvascular fluid absorption induced by an increase in the colloid osmotic pressure following a fixed intravenous bolus of human albumin was analyzed, first before start of, and then during an intra-arterial infusion to, the muscle preparation of the synthetic colloid to be analyzed. Capillary filtration coefficient as a measure of microvascular fluid permeability (conductance) was analyzed before and after start of the synthetic colloid.


RESULTS
Arterial blood flow, arterial and venous blood pressures, total vascular resistance, tissue volume changes, capillary filtration coefficient, and plasma volume were measured before and during the colloid infusion. According to the Starling fluid equilibrium, the ratio between the reflection coefficients for albumin on two occasions (before and after infusion of the synthetic colloid) can be calculated from the maximum osmotic absorption rates induced by a fixed intravenous bolus infusion of albumin and from the capillary filtration coefficients. Obtained data were adjusted for different plasma volume at the two occasions. We found that none of the three synthetic colloids analyzed had any significant effect on the reflection coefficient for albumin.


CONCLUSIONS
An effect on albumin microvascular permeability of the synthetic colloids dextran 70, HES 200/0.5, and urea-linked gelatin could not be shown by a method analyzing their effect on the reflection coefficient for albumin.
Holbeck
S
S
Department of Anaesthesia and Intensive Care, University Hospital of Lund, Sweden. staffan.holbeck@usa.net
Bentzer
P
P
Wikstrand
C
C
Grände
P O
PO
D000704:Analysis of Variance; D000818:Animals; D001783:Blood Flow Velocity / Q000187:drug effects; D002199:Capillary Permeability / Q000187:drug effects*; D002415:Cats; D003911:Dextrans / Q000494:pharmacology*; D005780:Gelatin / Q000494:pharmacology*; D006895:Hydroxyethyl Starch Derivatives / Q000494:pharmacology*; D018482:Muscle, Skeletal / Q000098:blood supply / Q000187:drug effects; D009997:Osmotic Pressure / Q000187:drug effects; D010952:Plasma Substitutes / Q000494:pharmacology*; D010953:Plasma Volume / Q000187:drug effects; D012709:Serum Albumin / Q000502:physiology*; D018709:Statistics, Nonparametric
D003911:Dextrans; D006895:Hydroxyethyl Starch Derivatives; D010952:Plasma Substitutes; D012709:Serum Albumin; D005780:Gelatin