Measles virus and canine distemper virus target proteins into a TAP-independent MHC class I-restricted antigen-processing pathway.
After infection of CEM174.T2 cells [deficient for the transporter of antigen presentation (TAP)] with measles virus (MV) the nucleocapsid protein is recognized by L(d)-restricted cytotoxic T cells in a TAP-independent, chloroquine-sensitive fashion. Presentation via the TAP-independent pathway requires virus replication. During MV infection of the cell the nucleocapsid as well as the matrix protein enter the endolysosomal compartment as indicated by colocalization with the lysosomal-associated membrane protein 1 (LAMP-1). Similarly, the nucleocapsid protein of canine distemper virus (CDV) is recognized in a TAP-independent fashion. In addition, a recombinant MV expressing bacterial beta-galactosidase protein is able to introduce the recombinant antigen into the TAP-independent pathway whereas a vaccinia virus expressing beta-galactosidase is not. These data and a report about TAP-independent recognition of parainfluenza virus type 1 suggest that members of the Paramyxoviridae family regularly introduce viral proteins into the TAP-independent antigen-processing pathway.
Neumeister
Claudia
C
Institute of Virology and Immunobiology, University of Würzburg, Versbacher Str. 7, 97078 Würzburg, Germany1.
Nanan
Ralph
R
Children Hospital, University of Würzburg, Josef-Schneider-Str. 2, 97080 Würzburg, Germany3.
Cornu
Tatjana I
TI
Institute of Molecular Biology, University of Zürich, Winterthurer Str. 190, 8057 Zürich, Switzerland2.
Lüder
Carsten G K
CGK
Institute of Hygiene and Microbiology, University of Würzburg, Josef-Schneider-Str. 2, 97080 Würzburg, Germany4.
Ter Meulen
Volker
V
Institute of Virology and Immunobiology, University of Würzburg, Versbacher Str. 7, 97078 Würzburg, Germany1.
Naim
Hussein
H
Institute of Molecular Biology, University of Zürich, Winterthurer Str. 190, 8057 Zürich, Switzerland2.
Niewiesk
Stefan
S
Institute of Virology and Immunobiology, University of Würzburg, Versbacher Str. 7, 97078 Würzburg, Germany1.
D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D015703:Antigens, CD / Q000378:metabolism; D000956:Antigens, Viral / Q000276:immunology / Q000378:metabolism; D002460:Cell Line; D002478:Cells, Cultured; D002738:Chloroquine / Q000494:pharmacology; D004217:Distemper Virus, Canine / Q000235:genetics / Q000276:immunology*; D011992:Endosomes / Q000187:drug effects / Q000378:metabolism; D000939:Epitopes / Q000276:immunology; D005822:Genetic Vectors / Q000235:genetics; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D007739:L Cells; D051907:Lysosomal Membrane Proteins; D008247:Lysosomes / Q000187:drug effects / Q000378:metabolism; D008459:Measles virus / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D019251:Nucleocapsid / Q000276:immunology / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014616:Vaccinia virus / Q000235:genetics / Q000276:immunology; D014763:Viral Matrix Proteins / Q000276:immunology / Q000378:metabolism; D014779:Virus Replication
D015703:Antigens, CD; D000956:Antigens, Viral; D000939:Epitopes; D015395:Histocompatibility Antigens Class I; D051907:Lysosomal Membrane Proteins; D008562:Membrane Glycoproteins; D011993:Recombinant Fusion Proteins; D014763:Viral Matrix Proteins; D002738:Chloroquine