The alpha/beta fold uracil DNA glycosylases: a common origin with diverse fates.
BACKGROUND
Uracil DNA glycosylases (UDGs) are major repair enzymes that protect DNA from mutational damage caused by uracil incorporated as a result of a polymerase error or deamination of cytosine. Four distinct families of UDGs have been identified, which show very limited sequence similarity to each other, although two of them have been shown to possess the same structural fold. The structural and evolutionary relationships between the rest of the UDGs remain uncertain.


RESULTS
Using sequence profile searches, multiple alignment analysis and protein structure comparisons, we show here that all known UDGs possess the same fold and must have evolved from a common ancestor. Although all UDGs catalyze essentially the same reaction, significant changes in the configuration of the catalytic residues were detected within their common fold, which probably results in differences in the biochemistry of these enzymes. The extreme sequence divergence of the UDGs, which is unusual for enzymes with the same principal activity, is probably due to the major role of the uracil-flipping caused by the conformational strain enacted by the enzyme on uracil-containing DNA, as compared with the catalytic action of individual polar residues. We predict two previously undetected families of UDGs and delineate a hypothetical scenario for their evolution.


CONCLUSIONS
UDGs form a single protein superfamily with a distinct structural fold and a common evolutionary origin. Differences in the catalytic mechanism of the different families combined with the construction of the catalytic pocket have, however, resulted in extreme sequence divergence of these enzymes.
Aravind
L
L
National Center for Biotechnology Information, National Institutes of Health, Bethesda, MD 20894, USA. aravind@ncbi.nlm.nih.gov
Koonin
E V
EV
D000595:Amino Acid Sequence; D000818:Animals; D001105:Archaea / Q000201:enzymology; D001419:Bacteria / Q000201:enzymology; D001665:Binding Sites; D002384:Catalysis; D019295:Computational Biology; D003198:Computer Simulation; D017124:Conserved Sequence; D045647:DNA Glycosylases; D019992:Databases as Topic; D019143:Evolution, Molecular; D020224:Expressed Sequence Tags; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009699:N-Glycosyl Hydrolases / Q000737:chemistry* / Q000145:classification / Q000378:metabolism*; D010802:Phylogeny; D017510:Protein Folding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D012984:Software; D051981:Uracil-DNA Glycosidase; D014780:Viruses / Q000201:enzymology
D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D051981:Uracil-DNA Glycosidase