title: Quantitative analysis by liquid chromatography-tandem mass spectrometry of deuterium-labeled and unlabeled vitamin E in biological samples.
abstract: A method for quantification of unlabeled alpha-tocopherol and the deuterated tocopherols, RRR-alpha-5-(CD(3))-tocopherol (d(3)RRR) and all rac-alpha-5,7-(CD(3))(2) tocopherol (d(6)all-rac) in plasma by HPLC-tandem mass spectrometry (LC-MS/MS) has been developed. Deuterated and unlabeled alpha-tocopherols were separated by HPLC and were detected by positive ion multiple-reaction monitoring using a triple-quadrupole mass spectrometer equipped with a heated nebulizer-atmospheric pressure chemical ionization interface, following routine extraction of vitamin E from plasma. The accuracy and precision were evaluated by replicate analysis of standards and samples. Human plasma samples, which were obtained at different times after the subject had consumed a capsule containing 1:1 ratio of d(3)RRR and d(6)all rac-alpha-tocopheryl acetates, were analyzed with this method. Plasma deuterated alpha-tocopherols measured by LC-MS/MS followed the same pattern as previously demonstrated by GC-MS measurement, without requiring an extra derivitization step. The detection limit was 10 pmol for each form of alpha-tocopherol injected.
authors.affiliation: Department of Animal Nutrition and Physiology, Danish Institute of Agricultural Sciences, Research Centre Foulum.
authors.forename: C
authors.initials: C
authors.lastname: Lauridsen
authors.forename: S W
authors.initials: SW
authors.lastname: Leonard
authors.forename: D A
authors.initials: DA
authors.lastname: Griffin
authors.forename: D C
authors.initials: DC
authors.lastname: Liebler
authors.forename: T D
authors.initials: TD
authors.lastname: McClure
authors.forename: M G
authors.initials: MG
authors.lastname: Traber
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003903:Deuterium; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results; D013552:Swine; D014810:Vitamin E / Q000097:blood*
chemical_list: D014810:Vitamin E; D003903:Deuterium