title: Characterization and pathogenetic role of proteinase from Acanthamoeba castellanii.
abstract: A secreted proteinase was purified from the culture supernatant of Acanthamoeba castellanii with several chromatographic steps. The purified proteinase was a chymotrypsin-like serine proteinase. Its molecular weight was approximately 12 kDa on SDS-PAGE, and its native molecular weight was 12 kDa when determined by molecular sieve chromatography. It showed a broad temperature optimum ranging 30-55 degrees C with an optimal at 55 degrees C and an optimal pH of 8.5. It could degrade various protein substrates, such as collagen, fibronectin, laminin, secretory immunoglobulin A, immunoglobulin G, plasminogen, fibrinogen, haemoglobin and rabbit corneal proteins. It showed strong cytopathic effects in cultured cells, including HEp2 and HEK cells. The corneal lesions, induced by both the purified proteinase and A. castellanii, displayed similar clinical results for both cases, in which the stromal infiltration and opacity with the epithelial defect were revealed. These results suggest that the enzyme was highly associated with the pathogenesis of Acanthamoeba. The fact that cytopathic effects and development of corneal lesions caused by the proteinase of Acanthamoeba were inhibited by the proteinase inhibitor suggest that the proteinase inhibitor might be useful as a therapeutic agent.
authors.affiliation: Department of Biology, Chung-Ang University, Seoul, 156-756, Korea.
authors.forename: B K
authors.initials: BK
authors.lastname: Na
authors.forename: J C
authors.initials: JC
authors.lastname: Kim
authors.forename: C Y
authors.initials: CY
authors.lastname: Song
mesh_terms: D000048:Acanthamoeba / Q000201:enzymology* / Q000472:pathogenicity*; D015823:Acanthamoeba Keratitis / Q000469:parasitology / Q000473:pathology*; D000818:Animals; D002478:Cells, Cultured; D003315:Cornea / Q000469:parasitology / Q000473:pathology; D010450:Endopeptidases / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D011480:Protease Inhibitors / Q000494:pharmacology; D011817:Rabbits; D013379:Substrate Specificity; D013696:Temperature; D014774:Virulence
chemical_list: D011480:Protease Inhibitors; D010450:Endopeptidases