title: Recent advances in large-scale transposon mutagenesis.
abstract: Transposons were identified as mobile genetic elements over fifty years ago and subsequently became powerful tools for molecular-genetic studies. Recently, transposon-mutagenesis strategies have been developed to identify essential and pathogenicity-related genes in pathogenic microorganisms. Also, a number of in vitro transposition systems have been used to facilitate genome sequence analysis. Finally, transposon mutagenesis of yeast and complex eukaryotes has provided valuable functional genomic information to complement genome-sequencing projects.
authors.affiliation: Paradigm Genetics Inc., 104 Alexander Drive, Building 2, Research Triangle Park, NC 27709, USA.
authors.forename: L
authors.initials: L
authors.lastname: Hamer
authors.forename: T M
authors.initials: TM
authors.lastname: DeZwaan
authors.forename: M V
authors.initials: MV
authors.lastname: Montenegro-Chamorro
authors.forename: S A
authors.initials: SA
authors.lastname: Frank
authors.forename: J E
authors.initials: JE
authors.lastname: Hamer
mesh_terms: D000818:Animals; D004251:DNA Transposable Elements / Q000235:genetics*; D018390:Gene Targeting / Q000379:methods; D006801:Humans; D016297:Mutagenesis, Site-Directed
chemical_list: D004251:DNA Transposable Elements