[Preliminary studies on outer membrane protein (OMP) patterns of Escherichia coli O1, O2 and O78 isolates of chicken origin].
Omp patterns of twenty-five isolates of Escherichia coli serotype O1, O2 and O78 of chicken origin from Jiangsu area were determined. The outer membrane proteins of these isolates were isolated with the improved N-lauroylsarcosine method and analyzed by SDS-PAGE. Major outer membrane proteins bands contributed for OMP patterns were visualized with Coomassie brilliant blue staining. Two OMP patterns were divided in eight of O1 isolates and nine O2 isolates respectively. Among these OMP patterns, 1 OMP pattern was shared by these 2 different O serotypes isolates, and eight of O78 isolates also shared this OMP pattern. These results indicated that the OMP patterns of avian pathogenic Escherichia coli isolates of O1, O2 and O78 from this area were heterogeneous, and the same OMP patterns was presented in these 3 serotypes.
Gao
S
S
Department of Animal Medicine, Agricultural College of Yangzhou University, Yanngzhou 225009.
Liu
X
X
Zhang
R
R
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification*; D002645:Chickens / Q000382:microbiology*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000737:chemistry* / Q000302:isolation & purification; D018740:Genetic Heterogeneity; D008970:Molecular Weight
D001425:Bacterial Outer Membrane Proteins