Validation of liquid-liquid extraction followed by flow-injection negative ion electrospray mass spectrometry assay to Topiramate in human plasma.
This paper describes the development and validation of a method for the quantitative analysis of Topiramate (2,3:4,5-bis-O-(1-methylethylidene)-beta-D-fructopyranose sulfamate), a new antiepileptic drug, in human plasma using liquid-liquid extraction followed by flow-injection negative ion electrospray mass spectrometry. Using Prednisone (1,4-pregnadiene-17-alpha,21-diol-3,11,20-trione [10 microg/mL]) as an internal standard, calibration curves for Topiramate were linear over a range of 1 to 30 microg/mL in human plasma and were highly reliable (r(2) = 0.9991). The lower limit of quantitation of the assay was 2 microg/mL in human plasma. Precision (%CV <15%) and accuracy (<20%) for both intra- and inter-day validations were satisfactory. The method has been used in clinical pharmacology research.
Chen
S
S
Department of Pharmacology, Rush Medical College, Chicago, Illinois, USA. schen@rush.edu
Carvey
P
P
D000927:Anticonvulsants / Q000097:blood*; D005632:Fructose / Q000031:analogs & derivatives* / Q000097:blood*; D013058:Mass Spectrometry / Q000379:methods*; D011241:Prednisone / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D000077236:Topiramate
D000927:Anticonvulsants; D000077236:Topiramate; D005632:Fructose; D011241:Prednisone