Transcriptome analysis of channel catfish (Ictalurus punctatus): genes and expression profile from the brain.
Expressed sequence tag (EST) analysis was conducted using a complementary DNA (cDNA) library made from the brain mRNA of channel catfish (Ictalurus punctatus). As part of our transcriptome analysis in catfish to develop molecular reagents for comparative functional genomics, here we report analysis of 1201 brain cDNA clones. Of the 1201 clones, 595 clones (49.5%) were identified as known genes by BLAST searches and 606 clones (50.5%) as unknown genes. The 595 clones of known gene products represent transcripts of 251 genes. These known genes were categorized into 15 groups according to their biological functions. The largest group of known genes was the genes involved in translational machinery (21.4%) followed by mitochondrial genes (6.2%), structural genes (3.1%), genes homologous to sequences of unknown functions (2.3%), enzymes (2.7%), hormone and regulatory proteins (2.5%), genes involved in immune systems (2.1%), genes involved in sorting, transport, and metal metabolism (1.8%), transcriptional factors and DNA repair proteins (1.6%), proto-oncogenes (1.2%), lipid binding proteins (1.2%), stress-induced genes (0.7%), genes homologous to human genes involved in mental diseases (0.6%), and development or differentiation-related genes (0.3%). The number of genes represented by the 606 clones of unknown genes is not known at present, but the high percentage of clones showing no homology to any known genes in the GenBank databases may indicate that a great number of novel genes exist in teleost brain.
Ju
Z
Z
The Fish Molecular Genetics and Biotechnology Laboratory, 203 Swingle Hall, Department of Fisheries and Allied Aquacultures and Program of Cell and Molecular Biosciences, Auburn University, AL, Auburn 36849, USA.
Karsi
A
A
Kocabas
A
A
Patterson
A
A
Li
P
P
Cao
D
D
Dunham
R
R
Liu
Z
Z
D000818:Animals; D001921:Brain / Q000378:metabolism; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015723:Gene Library; D007059:Ictaluridae / Q000235:genetics*; D008969:Molecular Sequence Data; D012366:RNA, Transfer, Val / Q000235:genetics; D012269:Ribosomal Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
D018076:DNA, Complementary; D004272:DNA, Mitochondrial; D012366:RNA, Transfer, Val; D012269:Ribosomal Proteins