Control of cell proliferation via transduction of sPLA(2)-I activity and possible PPAR activation at the nuclear level.
Pancreatic phospholipase A2 (PLA(2)-I) stimulates U(III) cells proliferation, a rat uterine cell line, after binding to membrane receptors, internalization and translocation. Here, we demonstrate that during these steps of internalization, PLA(2)-I retains its hydrolytic activity and thus could exert its proliferative effect via nuclear phospholipids hydrolysis. Since fatty acids and eicosanoids released by such activity are known to be ligands of PPAR, we study the expression of these nuclear receptors and demonstrate that, in the experimental conditions where PLA(2)-I stimulates U(III) cells proliferation, PLA(2)-I also regulates PPAR expression indicating a possible mechanism of its proliferative effect.
Specty
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INSERM U352, Laboratoire de Biochimie et Pharmacologie, INSA-Lyon, 20 Avenue A. Einstein, 69621 Villeurbanne Cedex, France.
Pageaux
J F
JF
Dauça
M
M
Lagarde
M
M
Laugier
C
C
Fayard
J M
JM
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D015777:Eicosanoids / Q000378:metabolism; D004789:Enzyme Activation; D005260:Female; D054501:Group II Phospholipases A2; D006868:Hydrolysis; D008856:Microscopy, Fluorescence; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D013997:Time Factors; D014157:Transcription Factors / Q000378:metabolism*; D014161:Transduction, Genetic; D014599:Uterus / Q000378:metabolism
D015777:Eicosanoids; D010743:Phospholipids; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors; D010741:Phospholipases A; D054501:Group II Phospholipases A2; D054467:Phospholipases A2