title: [Avian Enzephalomyelitis of poultry: Contribution on the reduction of animals during vaccine testing]
abstract: The testing of live avian enzephalomyelitis (AE) virus vaccines according to the prescriptions of the European Pharmacopþia (EP) requires a number of animal trials. The purpose of these trials presented is the replacement respectively of two of the animal trials. The first test to be replaced is the virus titration in eggs which includes the observation of the hatching and survival capacity of infected embryos. Therefore a non-competitive ELISA was developed which allows the quantitative determination of AE-virus preparations. The second test to be refined is the efficacy testing of AE-vaccines. The challenge described in the European Pharmacopþia shall be replaced by the quantification of the serological response of the chickens and their progeny after vaccination. Therefore the minimum amount of protecting humoral antibodies has to be defined. First trials compared the pathogenicity of two challenge strains and evaluated the kinetic of maternal antibodies in chickens derived from vaccinated hens.
authors.affiliation: Paul-Ehrlich-Institut, D-Langen.
authors.forename: Anja
authors.initials: A
authors.lastname: Langhardt
authors.forename: Laura
authors.initials: L
authors.lastname: Frank
authors.forename: Brigitte
authors.initials: B
authors.lastname: Küchler
authors.forename: Carmen
authors.initials: C
authors.lastname: Jungbäck