Follow-up study of intrahost HIV type 2 variability reveals discontinuous evolution of C2V3 sequences.
Sequence change dynamics in the highly polymorphic C2V3 region of the envelope glycoprotein gp105 coding sequence of HIV-2 was studied for two HIV-2 seropositive Guinean individuals over a 5-year period. Proviral DNA was amplified by PCR from uncultured peripheral blood mononuclear cells (PBMC), cloned, and sequenced. Amino acid sequence alignment and phylogenetic analysis showed distinct viral populations for the three collected samples (1992, 1995, and 1997) for both individuals. A discontinuous replacement of sequences suggests activation of latent viruses present in reservoirs, a process that has been documented for the HIV-1 infection but unreported so far for HIV-2. The nucleotide sequences presented in this work have been assigned accession numbers AJ400276 to AJ400319.
Esteves
A
A
Unidade de Virologia, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, P-1349-008 Lisboa, Portugal.
Piedade
J
J
Santos
C
C
Venenno
T
T
Canas-Ferreira
W F
WF
Parreira
R
R
D000595:Amino Acid Sequence; D004279:DNA, Viral / Q000032:analysis; D019143:Evolution, Molecular; D015686:Gene Products, env / Q000737:chemistry / Q000235:genetics*; D015752:Genes, env / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics*; D015699:HIV Envelope Protein gp120 / Q000235:genetics; D015658:HIV Infections / Q000821:virology*; D015498:HIV-2 / Q000145:classification / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics; D010802:Phylogeny; D011533:Proviruses; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D054299:env Gene Products, Human Immunodeficiency Virus
D004279:DNA, Viral; D015686:Gene Products, env; D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D010446:Peptide Fragments; D054299:env Gene Products, Human Immunodeficiency Virus; C069790:gp105 envelope protein, Human immunodeficiency virus 2