Topography of lacUV5 initiation complexes.
Formation of a transcriptionally competent open complex is a highly regulated multistep process involving at least two intermediates. The rate of formation and stability of the intermediate complexes often determine promoter strength. However, the detailed mechanism of formation of the open complex and the high resolution structures of these intermediates are not known. In this study the structures of the open and intermediate complexes formed on the lacUV5 promoter by Escherichia coli RNA polymerase were analyzed using 'zero length' DNA-protein cross-linking. In both the open and the intermediate complexes the core subunits (ss' and ss) interact with lacUV5 DNA in a similar way, forming DNA-protein contacts flanking the initiation site. At the same time, the recognition (sigma(70)) subunit closely interacts with the promoter only in the open complex. In combination with our previous results, the data suggest that during promoter recognition contacts of the sigma subunit with core RNA polymerase and promoter DNA are rearranged in concert. These rearrangements constitute a landmark of transition from the intermediate to the open complex, identifying the sigma subunit as a key player directing formation of the open complex.
Studitsky
V
V
Department of Biochemistry and Molecular Biology, Room 5123, Wayne State University School of Medicine, 540 East Canfield Avenue, Detroit, MI 48201, USA. vstudit@wayne.med.edu
Brodolin
K
K
Liu
Y
Y
Mirzabekov
A
A
D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D007763:Lac Operon / Q000235:genetics*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D021122:Protein Subunits; D013696:Temperature
D021122:Protein Subunits; D004247:DNA; D012321:DNA-Directed RNA Polymerases