Serum withdrawal causes apoptosis in SHSY 5Y cells.
A proportion of differentiated SH-SY5Y cells undergo cell death in response to withdrawal of serum. This death manifests the hallmark features of apoptosis including changes in nuclear morphology, processing and activation of caspase 3 and cleavage of the caspase 3 substrates acetyl-Asp-Glu-Val-Asp-aminomethylcoumarin and poly(ADP-ribose) polymerase. These findings represent the first demonstration of serum withdrawal induced apoptosis in SH-SY5Y cells. The reduction in viability induced by serum deprivation and assessed using an inhibitor of mitochondrial respiration can be partially inhibited by FK506, but FK506 does not prevent caspase 3 processing or cleavage of caspase 3 substrates. FK506 is also able to promote the viability of a small proportion of embryonic mouse sensory neurons following nerve growth factor-withdrawal induced apoptosis. FK506 did not promote viability in either cell type in the absence of serum- or nerve growth factor-withdrawal. These observations are consistent with a survival-promoting effect of FK506 in cultured neurons.
Macleod
M R
MR
Department of Pharmacology and Fujisawa Institute of Neurosciences in Edinburgh, University of Edinburgh, Scotland, Edinburgh, UK. malcolm.macleod@ed.ac.uk
Allsopp
T E
TE
McLuckie
J
J
Kelly
J S
JS
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D015153:Blotting, Western; D002470:Cell Survival / Q000187:drug effects; D003374:Coumarins; D016895:Culture Media, Serum-Free; D005456:Fluorescent Dyes; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology; D051379:Mice; D009474:Neurons / Q000502:physiology*; D009475:Neurons, Afferent / Q000187:drug effects; D009842:Oligopeptides; D016559:Tacrolimus / Q000494:pharmacology; D012668:Trigeminal Ganglion / Q000166:cytology / Q000187:drug effects
D003374:Coumarins; D016895:Culture Media, Serum-Free; D005456:Fluorescent Dyes; D007166:Immunosuppressive Agents; D009842:Oligopeptides; C102580:acetyl-aspartyl-glutamyl-valyl-aspartyl-amino-4-methylcoumarin; D016559:Tacrolimus