Biochemical and molecular characterization of corn (Zea mays L.) root elongases.
Root surfaces are protected against the soil environment by the deposition of lignin and suberin. In order to obtain more insight into the regulation of root suberin biosynthesis, elongases from primary roots of corn (Zea mays L.) seedlings were characterized. Elongase activities (acyl-CoA and ATP-dependent) were located in the microsomal fraction of the root cells. C(20), C(22) and C(24) fatty acids were detected as primary products of elongases. Preferred substrates of the acyl-CoA elongases were C(18:0)-CoA and C(20:0)-CoA. Applying a molecular approach, using PCR and degenerate primers derived from the sequences of known leaf and seed 3-ketoacyl-CoA synthases (KCSs), catalysing the first step of very-long-chain fatty acid synthesis, the cDNA of a putative root KCS was obtained showing high homology to known leaf and seed KCSs at the DNA and amino acid levels. Thus, our approach provides the first direct evidence for the presence and the activity of root elongases in Z. mays. Ongoing research is focusing on the molecular analysis and the regulation of KCS expression in roots in reaction to different environmental stimuli.
Schreiber
L
L
Julius-von-Sachs-Institute, University of Wuerzburg, Julius-von-Sachs-Platz 3, 97082 Wuerzburg, Germany. lukas@botanik.uni-wuerzburg.de
Skrabs
M
M
Hartmann
K
K
Becker
D
D
Cassagne
C
C
Lessire
R
R
D051080:Acyl-Carrier Protein S-Malonyltransferase; D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism*; D007700:Kinetics; D008861:Microsomes / Q000201:enzymology; D005810:Multigene Family; D018515:Plant Leaves / Q000201:enzymology; D018517:Plant Roots / Q000166:cytology / Q000201:enzymology; D016133:Polymerase Chain Reaction; D012639:Seeds / Q000201:enzymology; D013379:Substrate Specificity; D003313:Zea mays / Q000201:enzymology* / Q000235:genetics
D000217:Acyltransferases; D051080:Acyl-Carrier Protein S-Malonyltransferase