title: Interaction of coxsackievirus A21 with its cellular receptor, ICAM-1.
abstract: Coxsackievirus A21 (CAV21), like human rhinoviruses (HRVs), is a causative agent of the common cold. It uses the same cellular receptor, intercellular adhesion molecule 1 (ICAM-1), as does the major group of HRVs; unlike HRVs, however, it is stable at acid pH. The cryoelectron microscopy (cryoEM) image reconstruction of CAV21 is consistent with the highly homologous crystal structure of poliovirus 1; like other enteroviruses and HRVs, CAV21 has a canyon-like depression around each of the 12 fivefold vertices. A cryoEM reconstruction of CAV21 complexed with ICAM-1 shows all five domains of the extracellular component of ICAM-1. The known atomic structure of the ICAM-1 amino-terminal domains D1 and D2 has been fitted into the cryoEM density of the complex. The site of ICAM-1 binding within the canyon of CAV21 overlaps the site of receptor recognition utilized by rhinoviruses and polioviruses. Interactions within this common region may be essential for triggering viral destabilization after attachment to susceptible cells.
authors.affiliation: Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907-1392, USA.
authors.forename: C
authors.initials: C
authors.lastname: Xiao
authors.forename: C M
authors.initials: CM
authors.lastname: Bator
authors.forename: V D
authors.initials: VD
authors.lastname: Bowman
authors.forename: E
authors.initials: E
authors.lastname: Rieder
authors.forename: Y
authors.initials: Y
authors.lastname: He
authors.forename: B
authors.initials: B
authors.lastname: Hébert
authors.forename: J
authors.initials: J
authors.lastname: Bella
authors.forename: T S
authors.initials: TS
authors.lastname: Baker
authors.forename: E
authors.initials: E
authors.lastname: Wimmer
authors.forename: R J
authors.initials: RJ
authors.lastname: Kuhn
authors.forename: M G
authors.initials: MG
authors.lastname: Rossmann
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D020285:Cryoelectron Microscopy; D004770:Enterovirus / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D018799:Intercellular Adhesion Molecule-1 / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D011991:Receptors, Virus / Q000737:chemistry / Q000378:metabolism*
chemical_list: D011991:Receptors, Virus; D018799:Intercellular Adhesion Molecule-1