title: Kinetics of allantoin metabolism in sheep.
abstract: The kinetics of allantoin metabolism were studied in rumen-cannulated sheep by means of a single intravenous injection of [4,5-(14)C]allantoin. The decline in the specific radioactivity of allantoin in plasma following the injection of tracer was best described by a double exponential function, indicating that allantoin moves in and out of two or more kinetically distinct compartments. Sequestering of tracer in secondary or tertiary compartments in the body water appears likely to have resulted in overestimation of net flux of allantoin through the blood in the present study. In future studies, sampling of blood for several days after administration of tracer should alleviate this problem. About 80 % of the [(14)C]allantoin injected was recovered as [(14)C]allantoin in urine during the 12 h after tracer injection, increasing to 94 % after 4 d. Allantoin-C also passed through the blood bicarbonate pool, suggesting that allantoin is degraded in the gastrointestinal tract. A small amount of allantoin-C (4 % of the net flux of allantoin through the blood pool) was apparently degraded to form bicarbonate-C in the rumen and postruminally, and subsequently appeared in blood bicarbonate-C. Transfer of allantoin-C into the rumen via saliva was insignificant. In view of these findings, the net flux of allantoin through the blood should be a better predictor of rumen microbial outflow than urinary allantoin excretion, because urinary excretion of purine derivatives must be adjusted for conversion of allantoin-C to blood bicarbonate when used to predict the flow of microbial protein from the rumen.
authors.affiliation: School of Rural Science and Natural Resources, University of New England, Armidale, NSW 2351, Australia. lkahn2@metz.une.edu.au
authors.forename: L P
authors.initials: LP
authors.lastname: Kahn
authors.forename: J V
authors.initials: JV
authors.lastname: Nolan
mesh_terms: D000481:Allantoin / Q000097:blood / Q000378:metabolism*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D007275:Injections, Intravenous; D008297:Male; D011849:Radioactive Tracers; D012756:Sheep / Q000378:metabolism*
chemical_list: D011849:Radioactive Tracers; D000481:Allantoin