Preparation of desmopressin-containing liposomes for intranasal delivery.
The loading and leakage characteristics of the desmopressin-containing liposomes and the effect of liposomes on the nasal mucosa permeation and antidiuresis of desmopressin were investigated. Higher loading efficiency of desmopressin for positively charged liposomes than negatively charged liposomes was obtained, and neutral liposomes resulted in a similar loading efficiency as that of positively charged liposomes. Greater leakage of desmopressin from negatively charged liposomes than from positively charged and neutral liposomes was shown. The increase of permeability of desmopressin through the nasal mucosa indicated positively charged liposomes>negatively charged liposomes>solution. It was suggested that the enhanced contact time of positively charged liposomes with negatively charged nasal mucosa led to a high local desmopressin concentration on the penetration site to promote an effective penetration of desmopressin through the nasal mucosa. The desmopressin antidiuresis result after intranasal administration was in good agreement with the permeability result in the order of positively charged liposomes>negatively charged liposomes>solution. One of the mechanisms for the explanation of the best result on the enhancement of antidiuresis for positively charged liposomes may be the bioadhesive effect of the liposomes on the negatively charged nasal mucosa.
Law
S L
SL
Pharmaceutics Research Laboratory, Department of Medical Research and Education, Veterans General Hospital-Taipei, Shih-Pai, Taipei, Taiwan, ROC.
Huang
K J
KJ
Chou
H Y
HY
D000281:Administration, Intranasal; D000818:Animals; D003894:Deamino Arginine Vasopressin / Q000008:administration & dosage*; D004337:Drug Carriers; D005260:Female; D006863:Hydrogen-Ion Concentration; D008081:Liposomes / Q000008:administration & dosage*; D011817:Rabbits
D004337:Drug Carriers; D008081:Liposomes; D003894:Deamino Arginine Vasopressin