Phosphorylation of 1-deoxy-D-xylulose by D-xylulokinase of Escherichia coli.
1-deoxy-D-xylulose 5-phosphate serves as a precursor for the biosynthesis of the vitamins thiamine and pyridoxal and for the formation of isopentenyl pyrophosphate and dimethylallyl pyrophosphate via the nonmevalonate pathway of terpenoid biosynthesis. Earlier studies had shown that Escherichia coli incorporates unphosphorylated 1-deoxy-D-xylulose into the terpenoid side chain of ubiquinones with high efficacy. We show that D-xylulokinase of E. coli (EC 2.7.1.17) catalyzes the phosphorylation of 1-deoxy-D-xylulose at the hydroxy group of C-5 at a rate of 1.6 micromol.mg min-1. This reaction constitutes a potential salvage pathway for the generation of 1-deoxy-D-xylulose 5-phosphate from exogenous or endogenous 1-deoxy-D-xylulose as starting material for the biosynthesis of terpenoids, thiamine and pyridoxal.
Wungsintaweekul
J
J
Biozentrum-Pharmazie, Universität Halle, Halle/Saale, Germany.
Herz
S
S
Hecht
S
S
Eisenreich
W
W
Feicht
R
R
Rohdich
F
F
Bacher
A
A
Zenk
M H
MH
D000595:Amino Acid Sequence; D002247:Carbon Isotopes; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D010766:Phosphorylation; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid; D014996:Xylulose / Q000031:analogs & derivatives / Q000378:metabolism*
D002247:Carbon Isotopes; D011994:Recombinant Proteins; D014996:Xylulose; C029724:1-deoxy-2-pentulose; D017853:Phosphotransferases (Alcohol Group Acceptor); C032700:xylulokinase