Evaluation of a recombinant Listeria monocytogenes expressing an HIV protein that protects mice against viral challenge.
Vaccine strategies that utilize cell mediated immunity to control infection will be a necessary component of human immunodeficiency virus (HIV) vaccines. In previous studies we have shown that a Listeria monocytogenes recombinant expressing HIV-Gag elicits strong CD8+ and CD4+ T cell responses against HIV Gag in addition to its own secreted proteins. Here, we show that Lm-Gag can protect mice against a viral challenge with a recombinant vaccinia virus expressing Gag, in an antigen specific manner, and that this protection is T cell mediated. These results further support the use of L. monocytogenes as a vaccine approach for HIV through the induction of T cell immunity.
Mata
M
M
Department of Microbiology, University of Pennsylvania Medical School, 323 Johnson Pavilion, 3610 Hamilton Walk, Philadelphia, PA 19104-6076, USA.
Yao
Z J
ZJ
Zubair
A
A
Syres
K
K
Paterson
Y
Y
D016915:AIDS Vaccines / Q000235:genetics / Q000302:isolation & purification; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D017931:DNA Primers / Q000235:genetics; D005260:Female; D015683:Gene Products, gag / Q000235:genetics* / Q000276:immunology*; D006678:HIV / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D007114:Immunization; D008089:Listeria monocytogenes / Q000235:genetics* / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000276:immunology; D027383:Papillomaviridae / Q000235:genetics / Q000276:immunology; D011995:Recombination, Genetic
D016915:AIDS Vaccines; D017931:DNA Primers; D015683:Gene Products, gag; D009856:Oncogene Proteins, Viral