Distinct signals generate repeating striped pattern in the embryonic parasegment.
How repeating striped patterns arise across cellular fields is unclear. To address this we examined the repeating pattern of Stripe (Sr) expression across the parasegment (PS) in Drosophila. This pattern is generated in two steps. First, the ligands Hedgehog (Hh) and Wingless (Wg) subdivide the PS into smaller territories. Second, the ligands Hh, Spitz (Spi), and Wg each emanate from a specific territory and induce Sr expression in an adjacent territory. We also show that the width of Sr expression is determined by signaling strength. Finally, an enhancer trap in the sr gene detects the response to Spi and Wg, but not to Hh, implying the existence of separable control elements in the sr gene. Thus, a distinct inductive event is used to initiate each element of the repeating striped pattern.
Hatini
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Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA. hatiniv@mail.med.upenn.edu
DiNardo
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D000818:Animals; D019521:Body Patterning / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D004330:Drosophila; D029721:Drosophila Proteins; D005677:G(M1) Ganglioside / Q000031:analogs & derivatives*; D018507:Gene Expression Regulation, Developmental; D053823:Hedgehog Proteins; D017403:In Situ Hybridization; D019476:Insect Proteins / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D015398:Signal Transduction / Q000235:genetics*; D013110:Sphingosine / Q000031:analogs & derivatives*; D014157:Transcription Factors / Q000235:genetics*; D051155:Wnt1 Protein
D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D053823:Hedgehog Proteins; D019476:Insect Proteins; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; D051155:Wnt1 Protein; C096359:sr protein, Drosophila; C074622:wg protein, Drosophila; C086919:LIGA 20; C077245:hh protein, Drosophila; D005677:G(M1) Ganglioside; D013110:Sphingosine