title: Molecular composition of mitochondrial ATP-sensitive potassium channels probed by viral Kir gene transfer.
abstract: Heart cells contain ATP-sensitive potassium (KATP) channels in both the sarcolemma and the inner mitochondrial membrane. The sarcolemmal channels are believed to be heteromultimeric complexes of sulfonylurea receptors (SUR) and potassium inward rectifier (Kir) gene products, but the molecular identity of mitochondrial KATP (mitoKATP) channels remains unclear. To probe the molecular composition of KATP channels, we used adenoviral gene transfer to express wild-type (WT) and dominant-negative (AFA) constructs of Kir6.1 and 6.2 in rabbit ventricular myocytes. None of the Kir6.1 or 6.2 constructs affected mitoKATPchannel activity as assayed by confocal imaging of flavoprotein fluorescence, contradicting the proposal, based on subcellular antibody localization, that Kir6.1 forms part of mitoKATP channels. As previously reported, dominant-negative Kir6.2 gene transfer suppressed sarcolemmal KATP current, while Kir6.1 constructs had no effect on sarcolemmal activity. Immunohistochemistry with an anti-Kir6.1 antibody revealed expression of this protein in heart but no apparent co-localization with mitochondria. Thus, the available evidence indicates that both Kir6.1 and 6.2 are expressed in ventricular myocytes, but neither plays a discernible functional role in the mitoKATP channel.
authors.affiliation: Department of Medicine, Institute of Molecular Cardiobiology, Johns Hopkins University, Baltimore, MD 21205, USA.
authors.forename: J
authors.initials: J
authors.lastname: Seharaseyon
authors.forename: A
authors.initials: A
authors.lastname: Ohler
authors.forename: N
authors.initials: N
authors.lastname: Sasaki
authors.forename: H
authors.initials: H
authors.lastname: Fraser
authors.forename: T
authors.initials: T
authors.lastname: Sato
authors.forename: D C
authors.initials: DC
authors.lastname: Johns
authors.forename: B
authors.initials: B
authors.lastname: O'Rourke
authors.forename: E
authors.initials: E
authors.lastname: Marbán
mesh_terms: D000255:Adenosine Triphosphate / Q000502:physiology*; D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D002478:Cells, Cultured; D003981:Diazoxide / Q000494:pharmacology; D005420:Flavoproteins / Q000032:analysis; D005799:Genes, Dominant; D005822:Genetic Vectors / Q000235:genetics; D006352:Heart Ventricles / Q000166:cytology; D017136:Ion Transport; D008564:Membrane Potentials; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008929:Mitochondria, Heart / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D009206:Myocardium / Q000378:metabolism; D010084:Oxidation-Reduction; D011188:Potassium / Q000378:metabolism*; D015221:Potassium Channels / Q000235:genetics*; D024661:Potassium Channels, Inwardly Rectifying; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012508:Sarcolemma / Q000378:metabolism; D014162:Transfection
chemical_list: D005420:Flavoproteins; D015221:Potassium Channels; D024661:Potassium Channels, Inwardly Rectifying; D011993:Recombinant Fusion Proteins; D000255:Adenosine Triphosphate; D003981:Diazoxide; D011188:Potassium