title: A putative sodium-dependent inorganic phosphate co-transporter from Drosophila melanogaster.
abstract: We have isolated and sequenced a cDNA encoding a predicted 524 amino acid protein from a Drosophila melanogaster ovarian library. Sequence comparisons suggest that this protein encodes a sodium-dependent inorganic phosphate co-transporter similar to a sequence isolated from a rat brain library. In situ hybridisation to messenger RNA in ovaries shows strong expression in germarium at stage 2 of oogenesis. Expression is then weak in follicle cells until stage 10, when high transcript levels are seen in the nurse cells and transferred to the oocyte. This presumably reflects functions in oogenesis and the production of stored mRNAs for use in embryogenesis.
authors.affiliation: Institute of Cell and Molecular Biology, University of Edinburgh, King's Buildings, Mayfield Road, Edinburgh EH9 3JR, UK.
authors.forename: B
authors.initials: B
authors.lastname: MacIver
authors.forename: A
authors.initials: A
authors.lastname: McCahill
authors.forename: S
authors.initials: S
authors.lastname: Pathirana
authors.forename: K
authors.initials: K
authors.lastname: Leaper
authors.forename: M
authors.initials: M
authors.lastname: Bownes
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification*; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D008969:Molecular Sequence Data; D010053:Ovary / Q000378:metabolism; D051381:Rats; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D050608:Sodium-Phosphate Cotransporter Proteins; D027981:Symporters
chemical_list: D002352:Carrier Proteins; D050608:Sodium-Phosphate Cotransporter Proteins; D027981:Symporters