Role of the phosphoinositide pathway in the light-dependent C4 phosphoenolpyruvate carboxylase phosphorylation cascade in Digitaria sanguinalis protoplasts.
Stimulus-response coupling in animal cells frequently involves the hydrolysis of PtdIns(4,5)P(2) which is catalysed by phosphoinositide-specific phospholipase C (PI-PLC). There is an increasing body of evidence for PI-PLC-based signalling in plant cells; however, the physiological role of this system remains poorly documented in plants. Our data provide the first evidence that a PI-PLC-based signalling system is a committed step in the transduction chain controlling the phosphorylation state of C(4) phosphoenolpyruvate carboxylase (PEPC), the regulation of which is central to the assimilation of atmospheric CO(2) in C(4) plants.
Coursol
S
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Institut de Biotechnologie des Plantes, UMR 8618, Université Paris XI, Bâtiment 630, 91405 Orsay Cedex, France.
Pierre
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JN
Vidal
J
J
D002118:Calcium / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D008027:Light; D019684:Magnoliopsida / Q000201:enzymology / Q000502:physiology* / Q000528:radiation effects; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010730:Phosphoenolpyruvate Carboxylase / Q000378:metabolism*; D054801:Phosphoinositide Phospholipase C; D010766:Phosphorylation; D011523:Protoplasts / Q000201:enzymology*; D015398:Signal Transduction; D010738:Type C Phospholipases / Q000378:metabolism*
D002245:Carbon Dioxide; D010738:Type C Phospholipases; D054801:Phosphoinositide Phospholipase C; D010730:Phosphoenolpyruvate Carboxylase; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D002118:Calcium