title: Utilization of a novel deuterostome model for the study of regeneration genetics: molecular cloning of genes that are differentially expressed during early stages of larval sea star regeneration.
abstract: Sea stars share many characteristics with vertebrates, including deuterostome type development. We previously reported that sea star larvae are capable of complete regeneration (with organogenesis) of missing body parts. Here we report the first application of whole-body cDNA subtractive hybridization for the identification of regeneration-specific gene expression in a deuterostome. We identified nine novel cDNAs from genes differentially expressed during early larval sea star regeneration, including a serine protease which may have a function similar to that of trypsin/plasmin-like proteases during vertebrate wound repair and regeneration. This study demonstrates that sea star larvae can provide a valuable new deuterostome model for the study of regeneration genetics, with potential applications in vertebrate regeneration.
authors.affiliation: Department of Biology, The University of Alabama at Birmingham, 35294-1170, USA. mvickery@uab.edu
authors.forename: M C
authors.initials: MC
authors.lastname: Vickery
authors.forename: M S
authors.initials: MS
authors.lastname: Vickery
authors.forename: J B
authors.initials: JB
authors.lastname: McClintock
authors.forename: C D
authors.initials: CD
authors.lastname: Amsler
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D007814:Larva; D008969:Molecular Sequence Data; D012038:Regeneration / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases / Q000235:genetics*; D013215:Starfish / Q000235:genetics* / Q000254:growth & development
chemical_list: C429748:SRAP protein, Luidia foliolata; D012697:Serine Endopeptidases