[Genetic instability of microsatellites Mfd41 and DCC in the evolution of chronic myelogenous leukemia].
OBJECTIVE
To explore the relationship between the genetic instability of microsatellite and the evolution of chronic myelogenous leukemia(CML).


METHODS
The loss of heterozygosity(LOH) and the microsatellite instability(MSI) of two polymorphic microsatellite markers, DCC and Mfd41, which located on chromosome 18q and 17p respectively, were assayed by standard PCR-silver staining analysis in bone marrow cells from 17 CML patients progressing from chronic phase to accelerated phase or blast crisis.


RESULTS
LOH or MSI of the two microsatellites were demonstrated in 8/17 (47.5%) patients in accelerated/blastic phases. For DCC, genetic instability was revealed in 2 of 9 patients in accelerated phase and in 4 of 8 in blast crisis. For Mfd41, genetic instability was revealed in 1 of 9 patients in accelerated and in 1 of 8 in blast crisis.


CONCLUSIONS
Genetic instability of DCC and Mfd41 may play a role in the evolution of CML.
Li
G
G
Institute of Hematology, CAMS and PUMC, Tianjin 300020.
Song
Y
Y
Qian
L
L
D000293:Adolescent; D000328:Adult; D000368:Aged; D005260:Female; D016163:Genes, DCC; D016313:Genes, abl / Q000235:genetics; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D019656:Loss of Heterozygosity; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D014178:Translocation, Genetic