title: Systemic inflammation increases shear stress-induced platelet plug formation measured by the PFA-100.
abstract: The PFA-100 measures platelet plug formation under shear stress and is strongly dependent on von Willebrand Factor (VWF) levels in plasma. We therefore hypothesized that elevated VWF levels, possibly as a result of acute inflammation, adversely influence PFA-100 results. Healthy volunteers received either 2 ng/kg endotoxin or placebo in a randomized controlled trial. Four hours after endotoxin (but not placebo) infusion VWF levels increased by 85%, collagen epinephrine-induced closure time (CT) decreased by 47% and collagen ADP-CT decreased by 38% (P < 0.0001) respectively. In conclusion, systemic inflammation has a major impact on the results obtained by PFA-100 and may confound interpretation of platelet function.
authors.affiliation: Department of Clinical Pharmacology-TARGET, Vienna University Hospital School of Medicine, Vienna, Austria.
authors.forename: M
authors.initials: M
authors.lastname: Homoncik
authors.forename: A D
authors.initials: AD
authors.lastname: Blann
authors.forename: U
authors.initials: U
authors.lastname: Hollenstein
authors.forename: T
authors.initials: T
authors.lastname: Pernerstorfer
authors.forename: H G
authors.initials: HG
authors.lastname: Eichler
authors.forename: B
authors.initials: B
authors.lastname: Jilma
mesh_terms: D000208:Acute Disease; D000244:Adenosine Diphosphate; D000328:Adult; D004837:Epinephrine; D006801:Humans; D007249:Inflammation / Q000097:blood*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D010976:Platelet Count; D010979:Platelet Function Tests; D012680:Sensitivity and Specificity; D014841:von Willebrand Factor / Q000502:physiology*
chemical_list: D008070:Lipopolysaccharides; D014841:von Willebrand Factor; D000244:Adenosine Diphosphate; D004837:Epinephrine