title: Thermostable alpha-amylase production by an extreme thermophile Bacillus thermooleovorans.
abstract: OBJECTIVE
  alpha-Amylase production by a newly isolated thermophile, Bacillus thermooleovorans, was studied under different cultivation conditions.
  
  
  RESULTS
  The influence of various carbon and nitrogen sources on alpha-amylase production was quantified in batch fermentation in shake flasks. Starch and tryptone were observed to be the ideal carbon and nitrogen sources, respectively. Cultivation of the organism in a chemically defined medium consisting of glucose, riboflavin, cysteine, MgSO4, K2HPO4 and NaCl led to a near twofold increase in the production of alpha-amylase in comparison with that in the complex medium. The increase in enzyme production was achieved using vitamins and amino acids. When the organism was grown in a laboratory fermenter in the optimized complex medium, the noticeable effects were the near abolition of the lag phase, a 2.2-fold increase in enzyme production and a reduction in optimal production time from 12 to 4-5 h.
  
  
  CONCLUSIONS
  Enhancement of amylase production was achieved under various cultivation conditions.
  
  
  CONCLUSIONS
  Bacillus thermooleovorans produces a calcium-independent and thermostable amylase which can find use in starch saccharification.
authors.affiliation: Department of Microbiology, University of Delhi, South Campus, New Delhi, India.
authors.forename: S
authors.initials: S
authors.lastname: Narang
authors.forename: T
authors.initials: T
authors.lastname: Satyanarayana
mesh_terms: D001407:Bacillus / Q000201:enzymology* / Q000254:growth & development; D002244:Carbon / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D004795:Enzyme Stability; D005285:Fermentation; D006358:Hot Temperature; D009584:Nitrogen / Q000378:metabolism; D013213:Starch / Q000378:metabolism; D000516:alpha-Amylases / Q000096:biosynthesis*
chemical_list: D003470:Culture Media; D002244:Carbon; D013213:Starch; D000516:alpha-Amylases; D009584:Nitrogen