title: Mapping epitopes of monoclonal antibodies against HIV-1 integrase with limited proteolysis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry.
abstract: Monoclonal antibodies (mAbs) have been used extensively in the biochemical analysis of proteins. Molecular identification of a specific epitope can enhance our understanding of the relationship between the structure and function of a protein. We recently developed a protein footprint technique for mapping mAb epitopes that employs limited proteolysis followed by peptide analysis with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Here we describe the rational for the technique and illustrate its use in mapping the epitopes of two mAbs that bind to the C-terminal domain of human immunodeficiency virus type-1 integrase. The results provide a plausible explanation for the fact that one mAb inhibits enzyme activity while the second does not.
authors.affiliation: Fox Chase Cancer Center, Institute for Cancer Research, 7701 Burholme Avenue, Philadelphia, PA 19111, USA.
authors.forename: J
authors.initials: J
authors.lastname: Yi
authors.forename: A M
authors.initials: AM
authors.lastname: Skalka
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D010450:Endopeptidases; D018604:Epitope Mapping; D015483:HIV Antibodies; D015488:HIV Antigens / Q000737:chemistry / Q000235:genetics; D019427:HIV Integrase / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000911:Antibodies, Monoclonal; D015483:HIV Antibodies; D015488:HIV Antigens; D019427:HIV Integrase; D010450:Endopeptidases