[Bianticollagen membrane: preparation and analysis of properties].
This study was undertaken to assess the antiplaque and anticollagenase properties of bianticollagen membrane (BACM) for use in the guided tissue regeneration (GTR). First, preparing cross-linked collagen membrane (GLCM) from bone collagen by glutaraldehyde and ultraviolet irradiation. Then, the GLCM was coated with tetracycline (TC) delivery device, which is BACM. BACM's properties are as follows: under scan electron microscope (SEM), it is a three dimensional structure with small pores; the modulus of elasticity in low strain regions and swelling ratio are 20.4 g/mm2 and 0.141 respectively; immediate type and delayed type hypersensitivity are negative; BACM with TC 150 micrograms placed into pockets, the average intrasulcular TC concentration measured at the end of the 7-days is 46.76 +/- 5.69 micromol/L, which is 2 times higher than MIC of TC; the period against collagenase (100 u) digestion is over 30 days in vitro; 1 mg GLCM and BACM implanted in mice may maintain about 4 weeks and 7 weeks respectively, and BACM and GLCM placed onto patient's root surface in flap operation and taken at 7th day the bacteria on BACM are significantly less than that on GLCM under SEM. These results indicate that BACM has antiplaque and stronger antidegradation effects than GLCM.
Li
C
C
Stomatological College, Hubei Medical University, Wuhan 430070.
Fan
M
M
D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D003094:Collagen; D017364:Collagenases / Q000378:metabolism; D003692:Delayed-Action Preparations; D016557:Guided Tissue Regeneration, Periodontal; D006801:Humans; D008567:Membranes, Artificial; D051379:Mice; D010514:Periodontal Pocket / Q000378:metabolism; D013752:Tetracycline / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D003692:Delayed-Action Preparations; D008567:Membranes, Artificial; D003094:Collagen; D017364:Collagenases; D013752:Tetracycline