title: High-resolution genetic mapping of the saccharin preference locus (Sac) and the putative sweet taste receptor (T1R1) gene (Gpr70) to mouse distal Chromosome 4.
abstract: The Sac (saccharin preference) locus affecting mouse behavioral and neural responsiveness to sweeteners has been mapped to distal Chr 4. A putative sweet taste receptor, T1R1, has been recently cloned, and the gene encoding it, Gpr70, has also been mapped to mouse distal Chr 4. To assess Gpr70 as a candidate gene for Sac, we compared the Gpr70 sequences of C57BL/6ByJ and 129P3/J mouse strains with different alleles of Sac. Using Gpr70 sequence variation between the C57BL/6ByJ and 129P3/J strains, we conducted a high-resolution analysis of the chromosomal localization of the Gpr70 and Sac loci in the F2 hybrids and 129.B6-Sac partially congenic mice originating from these two strains. The Gpr70 gene maps proximal to Sac, which demonstrates that they are different loci.
authors.affiliation: Monell Chemical Senses Center, Philadelphia, Pennsylvania 19104, USA.
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: M
authors.initials: M
authors.lastname: Inoue
authors.forename: D R
authors.initials: DR
authors.lastname: Reed
authors.forename: T
authors.initials: T
authors.lastname: Huque
authors.forename: R B
authors.initials: RB
authors.lastname: Puchalski
authors.forename: M G
authors.initials: MG
authors.lastname: Tordoff
authors.forename: Y
authors.initials: Y
authors.lastname: Ninomiya
authors.forename: G K
authors.initials: GK
authors.lastname: Beauchamp
authors.forename: A A
authors.initials: AA
authors.lastname: Bachmanov
mesh_terms: D000818:Animals; D001483:Base Sequence; D002814:Chorda Tympani Nerve / Q000502:physiology; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D005260:Female; D008040:Genetic Linkage; D006239:Haplotypes; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D011956:Receptors, Cell Surface / Q000235:genetics*; D043562:Receptors, G-Protein-Coupled; D012439:Saccharin; D016415:Sequence Alignment; D013649:Taste / Q000235:genetics*
chemical_list: D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; C431926:taste receptors, type 1; D012439:Saccharin