title: Effects of transfusion on gastrointestinal anastomotic wound healing and leukocyte function in rats.
abstract: BACKGROUND
  This study was done to investigate the effects of blood transfusion on gastrointestinal anastomotic wound healing and leukocyte function.
  
  
  METHODS
  Closed-colony male Wistar rats were divided into five groups in a group-mixed fashion and underwent laparotomy (the CONT and WB groups) or gastrectomy and gastroduodenostomy (the GAST, GAST/WB and GAST/LDRWB groups). Immediately, they received 3 ml of saline (the CONT and GAST groups) or blood from Wistar donors (the WB and GAST/WB groups, whole blood; the GAST/LDRWB group, a transfusion of irradiated and leukocyte-depleted whole blood). The animals were killed 7 days after surgery.
  
  
  RESULTS
  The GAST/WB group had a higher incidence of anastomotic abscess, a lower level of factor XIII and a lower maximum breaking strength than the GAST group. Gastrectomy decreased L-DCL (luminol-dependent chemiluminescence) more than whole-blood transfusion. In the GAST/WB group, L-DCL was severely reduced, while in the GAST/LDRWB group it was roughly equivalent to the level in the GAST group.
  
  
  CONCLUSIONS
  Whole-blood transfusion increased the incidence of anastomotic abscess and impaired anastomotic wound healing, and these might relate to the reduction of L-DCL. Irradiated and leukocyte-depleted transfusion can improve these effects.
authors.affiliation: Second Department of Surgery, Faculty of Medicine, Gunma University, Maebashi, Japan. sohwada@sb.gumma-u.ac.jp
authors.forename: S
authors.initials: S
authors.lastname: Ohwada
authors.forename: Y
authors.initials: Y
authors.lastname: Sato
authors.forename: N
authors.initials: N
authors.lastname: Sato
authors.forename: Y
authors.initials: Y
authors.lastname: Toyama
authors.forename: T
authors.initials: T
authors.lastname: Okano
authors.forename: Y
authors.initials: Y
authors.lastname: Nakasone
authors.forename: T
authors.initials: T
authors.lastname: Ogawa
authors.forename: Y
authors.initials: Y
authors.lastname: Morishita
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D001770:Blood Bactericidal Activity; D001777:Blood Coagulation; D004385:Duodenostomy; D005176:Factor XIII / Q000378:metabolism; D005774:Gastrostomy; D006400:Hematocrit; D007202:Indicators and Reagents; D007962:Leukocytes / Q000502:physiology*; D008163:Luminescent Measurements; D008297:Male; D009930:Organic Chemicals; D051381:Rats; D017208:Rats, Wistar; D065227:Transfusion Reaction; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
chemical_list: D007202:Indicators and Reagents; D009930:Organic Chemicals; C013014:hepaplastin; D005176:Factor XIII