[Study on changing of intracellular free Ca2+ induced by ACh in outer hair cells of guinea pig using Fura-2 microfluorimetry].
The method of studying intracellular free Ca2+ ([Ca2+]i) in outer hair cells (OHC) of guinea pig using digital imaging microscopy and Ca(2+)-sensitive fluorescent dye Fura-2 was introduced. [Ca2+]i can be calculated from fluorescence intensity ratios with excitation lights of two different wave-lengths, using Fura-2 loaded in OHC. The data showed that acetylcholine (ACh) can induce [Ca2+]i elevation of OHC in the solution with calcium. Mechanism of ACh inducing [Ca2+]i elevation and application of calcium imaging microfluorometry for studying cochlea hair cell were discussed.
Yang
J
J
Department of Otorhinolaryngology, Union Hospital, Tongji Medical University, Wuhan 430022.
Wang
J
J
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D003592:Cytophotometry; D016257:Fura-2; D006168:Guinea Pigs; D018072:Hair Cells, Auditory, Outer / Q000378:metabolism*
D000109:Acetylcholine; D002118:Calcium; D016257:Fura-2