Cytogenetic analysis from DNA by comparative genomic hybridization.
Comparative genomic hybridization (CGH) is a modified in situ hybridization technique which allows detection and mapping of DNA sequence copy differences between two genomes in a single experiment. In CGH analysis, two differentially labelled genomic DNA (study and reference) are co-hybridized to normal metaphase spreads. Chromosomal locations of copy number changes in the DNA segments of the study genome are revealed by a variable fluorescence intensity ratio along each target chromosome. Since its development, CGH has been applied mostly as a research tool in the field of cancer cytogenetics to identify genetic changes in many previously unknown regions. CGH may also have a role in clinical cytogenetics for detection and identification of unbalanced chromosomal abnormalities.
Tachdjian
G
G
Service de biologie du développement et de la reproduction-cytogénétique, hôpital Antoine-Béclère, 92140 Clamart, France. gerard.tachdjian@abc.ap-hop-paris.fr
Aboura
A
A
Lapierre
J M
JM
Viguié
F
F
D002478:Cells, Cultured; D002869:Chromosome Aberrations; D002874:Chromosome Mapping / Q000379:methods*; D003582:Cytogenetics / Q000379:methods*; D004247:DNA / Q000097:blood / Q000235:genetics* / Q000302:isolation & purification; D003627:Data Interpretation, Statistical; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D007621:Karyotyping
D004247:DNA