title: Development of a quality control material for the measurement of 8-oxo-7,8-dihydro-2'-deoxyguanosine, an in vivo marker of oxidative stress, and comparison of results from different laboratories.
abstract: The measurement of 8-oxo-7,8-dihydro-2'-deoxyguanosine is an increasingly popular marker of in vivo oxidative damage to DNA. A random-sequence 21-mer oligonucleotide 5'-TCA GXC GTA CGT GAT CTC AGT-3' in which X was 8-oxo-guanine (8-oxo-G) was purified and accurate determination of the oxidised base was confirmed by a 32P-end labelling strategy. The lyophilised material was analysed for its absolute content of 8-oxo-dG by several major laboratories in Europe and one in Japan. Most laboratories using HPLC-ECD underestimated, while GC-MS-SIM overestimated the level of the lesion. HPLC-ECD measured the target value with greatest accuracy. The results also suggest that none of the procedures can accurately quantitate levels of 1 in 10(6) 8-oxo-(d)G in DNA.
authors.affiliation: MRC Centre for Mechanisms in Human Toxicity, Leicester, UK. jl20@le.ac.uk
authors.forename: J
authors.initials: J
authors.lastname: Lunec
authors.forename: K E
authors.initials: KE
authors.lastname: Herbert
authors.lastname: Jones GDD
authors.forename: L
authors.initials: L
authors.lastname: Dickinson
authors.forename: M
authors.initials: M
authors.lastname: Evans
authors.forename: N
authors.initials: N
authors.lastname: Mistry
authors.forename: P
authors.initials: P
authors.lastname: Mistry
authors.forename: D
authors.initials: D
authors.lastname: Chauhan
authors.forename: G
authors.initials: G
authors.lastname: Capper
authors.forename: Q
authors.initials: Q
authors.lastname: Zheng
mesh_terms: D015415:Biomarkers / Q000032:analysis*; D002853:Chromatography, Liquid; D019411:Clinical Laboratory Techniques; D003848:Deoxyguanine Nucleotides / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D009841:Oligonucleotides / Q000737:chemistry; D018384:Oxidative Stress; D011786:Quality Control; D017422:Sequence Analysis, DNA; D013056:Spectrophotometry, Ultraviolet
chemical_list: D015415:Biomarkers; D003848:Deoxyguanine Nucleotides; D009841:Oligonucleotides; C078206:8-oxodeoxyguanosine triphosphate