Microtubule-independent motility and nuclear targeting of adenoviruses with fluorescently labeled genomes.
A novel adenovirus system for analyzing the adenovirus entry pathway has been developed that contains green fluorescent protein bound to the encapsidated viral DNA (AdLite viruses). AdLite viruses enter host cells and accumulate around the nuclei and near the microtubule organizing centers (MTOC). In live cells, individual AdLite particles were observed trafficking both toward and away from the nucleus. Depolymerization of microtubules during infection prevented AdLite accumulation around the MTOC; however, it did not abolish perinuclear localization of AdLite particles. Furthermore, depolymerization of microtubules did not affect AdLite motility and did not affect gene expression from wild-type adenovirus and adenovirus-derived vectors. These data revealed that adenovirus intracellular motility and nuclear targeting can be supported by a mechanism that does not rely on the microtubule network.
Glotzer
J B
JB
Institute for Molecular Pathology, 1030 Vienna, Austria.
Michou
A I
AI
Baker
A
A
Saltik
M
M
Cotten
M
M
D000258:Adenovirus Infections, Human / Q000821:virology; D000260:Adenoviruses, Human / Q000235:genetics* / Q000472:pathogenicity* / Q000502:physiology; D002460:Cell Line; D002467:Cell Nucleus / Q000821:virology*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D016679:Genome, Viral; D049452:Green Fluorescent Proteins; D006367:HeLa Cells; D006801:Humans; D017403:In Situ Hybridization; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D018715:Microscopy, Video; D022101:Microtubule-Organizing Center; D008870:Microtubules / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000378:metabolism
D004268:DNA-Binding Proteins; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins