title: Selective activation and expansion of high-affinity CD4+ T cells in resistant mice upon infection with Leishmania major.
abstract: Using multimers of MHC class II molecules linked to a peptide derived from the Leishmania LACK antigen, we have compared the fate of parasite-specific CD4+ T cells in resistant and susceptible mice transgenic for the beta chain of a LACK-specific TCR. Activated T cells were readily detected in the draining lymph nodes of infected animals. Although the kinetics of activation and expansion were similar in both strains, T cells from susceptible and resistant mice expressed low- and high-affinity TCR, respectively. As T cells from resistant mice produced more IFN-gamma and less IL-4 than those from susceptible animals, our results suggest that differences in TCR usage between MHC-matched animals may influence the development of the antiparasite immune response.
authors.affiliation: Centre National de la Recherche Scientifique, University of Nice-Sophia Antipolis, Valbonne, France.
authors.forename: L
authors.initials: L
authors.lastname: Malherbe
authors.forename: C
authors.initials: C
authors.lastname: Filippi
authors.forename: V
authors.initials: V
authors.lastname: Julia
authors.forename: G
authors.initials: G
authors.lastname: Foucras
authors.forename: M
authors.initials: M
authors.lastname: Moro
authors.forename: H
authors.initials: H
authors.lastname: Appel
authors.forename: K
authors.initials: K
authors.lastname: Wucherpfennig
authors.forename: J C
authors.initials: JC
authors.lastname: Guéry
authors.forename: N
authors.initials: N
authors.lastname: Glaichenhaus
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D016207:Cytokines / Q000378:metabolism; D019281:Dimerization; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007113:Immunity, Innate / Q000276:immunology; D007700:Kinetics; D018320:Leishmania major / Q000276:immunology*; D016773:Leishmaniasis, Cutaneous / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000276:immunology*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics / Q000276:immunology; D013205:Staphylococcal Protein A / Q000378:metabolism
chemical_list: D000953:Antigens, Protozoan; D016207:Cytokines; D000949:Histocompatibility Antigens Class II; C111389:I-Ad antigen; D015800:Protozoan Proteins; D016693:Receptors, Antigen, T-Cell, alpha-beta; D013205:Staphylococcal Protein A; C093139:LACK antigen, Leishmania