title: Comparative sensitivity of six serological tests and diagnostic value of ELISA using purified antigen in hydatidosis.
abstract: Most serodiagnostic techniques have been evaluated for diagnosis of cystic hydatid disease caused by Echinococcus granulosus. Each, to varying degrees, has been shown to give false results, with considerable variation between laboratories. The comparative study was made concerning the sensitivity of the immunodiagnostic methods based on 58 sera from hydatid disease with different cyst locations. Latex agglutination, immunoelectrophoresis (IEP), and specific IgE, IgG enzyme-linked immunosorbent assay (ELISA) tests were studied. Specific IgG ELISA AgB (antigen B-rich fraction) was the most sensitive test (96.5%) and the least sensitive tests were specific IgE ELISA (24.1%) and IEP (25.8%). The low sensitivity of these two tests was due partly to the low reactivity detected in the sera of patients with lung hydatidosis. Initial laboratory studies showed purified antigens to be preferable to crude cyst fluid, regardless of the type of test used. For this reason, we evaluated the sensitivity and specificity of ELISA by using the purified antigen-B-rich fraction. In all, 117 sera were examined: 78 sera from patients with hydatidosis surgically confirmed, 15 sera from healthy control subjects, and 24 sera from patients with diseases other than hydatidosis. The method gave good results: 93.5% sensitivity, 89.7% specificity, and 92.3% diagnostic efficacy.
authors.affiliation: Instituto de Biotecnología, Facultad de Ciencias, Universidad de Granada, Granada, Spain.
authors.forename: Y
authors.initials: Y
authors.lastname: Sbihi
authors.forename: A
authors.initials: A
authors.lastname: Rmiqui
authors.forename: M N
authors.initials: MN
authors.lastname: Rodriguez-Cabezas
authors.forename: A
authors.initials: A
authors.lastname: Orduña
authors.forename: A
authors.initials: A
authors.lastname: Rodriguez-Torres
authors.forename: A
authors.initials: A
authors.lastname: Osuna
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000276:immunology*; D020420:Cyst Fluid / Q000276:immunology / Q000469:parasitology; D004443:Echinococcosis / Q000175:diagnosis*; D004444:Echinococcosis, Hepatic / Q000175:diagnosis; D004445:Echinococcosis, Pulmonary / Q000175:diagnosis; D004446:Echinococcus / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005188:False Negative Reactions; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G