The three-dimensional structure of cytosolic bovine retinal creatine kinase.
Creatine kinase (CK) catalyses the reversible transfer of the phosphate moiety from phosphocreatine (PCr) to ADP, generating creatine and ATP. The crystal structure of a cytosolic brain-type creatine kinase is reported at 2.3 A. The biological dimer sits on a crystallographic twofold axis. The N-terminal residues of both subunits come very close to the crystallographic twofold at the dimer interface. The electron density observed is consistent with two alternative conformations for the N-termini, as previously found for chicken brain-type creatine kinase.
Tisi
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Department of Crystallography, Birkbeck College, Malet Street, London WC1 7HX, England.
Bax
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Loew
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D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000201:enzymology; D002417:Cattle; D002645:Chickens; D003402:Creatine Kinase / Q000737:chemistry*; D018360:Crystallography, X-Ray; D003600:Cytosol / Q000201:enzymology; D019281:Dimerization; D006801:Humans; D007527:Isoenzymes / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010786:Photoreceptor Cells / Q000201:enzymology; D017433:Protein Structure, Secondary; D021122:Protein Subunits; D012160:Retina / Q000201:enzymology*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D055672:Static Electricity
D007527:Isoenzymes; D021122:Protein Subunits; D003402:Creatine Kinase