Occurrence of ceramides and neutral glycolipids with unusual long-chain base composition in purified rat liver mitochondria.
The free ceramide content of rat liver mitochondria was found to be 1.7 nmol/mg protein and outer membranes contained a three-fold higher concentration than inner membranes. The mitochondrial content in neutral glycolipids was 0.6 nmol/mg protein. The long-chain bases found in free ceramides were d18:1 sphingosine, d18:0 3-ketosphinganine and t21:1 phytosphingosine in increasing order. In contrast, 3-ketosphinganine was the only base of glucosylceramide and lactosylceramide of inner membranes, whereas d18:1 sphingosine was the major long-chain base of glucosylceramide of outer membranes.
Ardail
D
D
INSERM U189, Faculty of Medicine Lyon-Sud, Ouillins, France.
Popa
I
I
Alcantara
K
K
Pons
A
A
Zanetta
J P
JP
Louisot
P
P
Thomas
L
L
Portoukalian
J
J
D000818:Animals; D015703:Antigens, CD; D002518:Ceramides / Q000032:analysis* / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D005963:Glucosylceramides / Q000032:analysis / Q000737:chemistry; D007425:Intracellular Membranes / Q000737:chemistry; D007790:Lactosylceramides / Q000032:analysis / Q000737:chemistry; D008930:Mitochondria, Liver / Q000737:chemistry*; D020383:Neutral Glycosphingolipids / Q000032:analysis* / Q000737:chemistry*; D051381:Rats; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000032:analysis
D015703:Antigens, CD; D002518:Ceramides; D005227:Fatty Acids; D005963:Glucosylceramides; D007790:Lactosylceramides; D020383:Neutral Glycosphingolipids; C002882:ketodihydrosphingosine; C009744:CDw17 antigen; C012491:phytosphingosine; D013110:Sphingosine