Epigenetic change in IGF2R is associated with fetal overgrowth after sheep embryo culture.
Manipulation or non-physiological embryo culture environments can lead to defective fetal programming in livestock. Our demonstration of reduced fetal methylation and expression of ovine IGF2R suggests pre-implantation embryo procedures may be vulnerable to epigenetic alterations in imprinted genes. This highlights the potential benefits of epigenetic diagnostic screening in developing embryo procedures.
Young
L E
LE
Roslin Institute (Edinburgh), Roslin, Midlothian, Scotland, UK. Lorraine.Young@bbsrc.ac.uk
Fernandes
K
K
McEvoy
T G
TG
Butterwith
S C
SC
Gutierrez
C G
CG
Carolan
C
C
Broadbent
P J
PJ
Robinson
J J
JJ
Wilmut
I
I
Sinclair
K D
KD
D000015:Abnormalities, Multiple / Q000662:veterinary; D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D019976:Cloning, Organism / Q000662:veterinary*; D000013:Congenital Abnormalities / Q000662:veterinary; D019175:DNA Methylation; D005314:Embryonic and Fetal Development; D005260:Female; D005820:Genetic Testing; D018392:Genomic Imprinting; D011247:Pregnancy; D017527:Receptor, IGF Type 2 / Q000235:genetics*; D012756:Sheep
D017527:Receptor, IGF Type 2