Cytokine characteristics of jaundice in mouse liver.
OBJECTIVE
The aim of this study is to clarify the perioperative cytokine changes and their mechanism in jaundiced liver.


METHODS
Obstructive jaundice was induced using a common bile duct ligation (CBDL) and a two-thirds hepatectomy (HEP) was performed in six- to seven-week-old male C3H/HeN mice. When hepatectomy was added to CBDL, it was carried out 2 to 5 days after CBDL. The serum interleukin 6 (IL-6) levels and heat shock protein (HSP)-70 expression were evaluated. One mg per mouse of methylprednisolone (MPL) was intraperitonealy administered in some mice of CBDL+HEP group.


RESULTS
The post-hepatectomy IL-6 values at 2 and 3 days after CBDL were significantly lower than those in the HEP group, while those at 5 days after CBDL were significantly higher than those in HEP group. The serum IL-6 value of the steroid group was significantly lower than that of non-steroid group in HEP group. However, no steroid effects were recognized on post-hepatectomy IL-6 values at 3 days after CBDL, steroid inhibited post-hepatectomy IL-6 production at 5 days after CBDL. No expression of HSP70 protein was observed in the control group, but HSP70 protein was expressed in both the hepatocytes and Kupffer cells 3 days after CBDL, then at 5 days after CBDL, no HSP70 protein was expressed in the Kupffer cells.


CONCLUSIONS
In the early period of obstructive jaundice, the IL-6 level after hepatectomy did not increase in comparison to HEP group, and steroid had no effect on IL-6 level. According to the progression of obstructive jaundice, the IL-6 level after hepatectomy increased to a higher level than before, and the effect of MPL was restored. HSP70 is thus suggested to have an important role in cytokine production.
Fujiwara
Y
Y
Second Department of Surgery, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Fukuoka, Higashi-ku, 812-8582, Japan.
Shimada
M
M
Yamashita
Y
Y
Adachi
E
E
Shirabe
K
K
Takenaka
K
K
Sugimachi
K
K
D000818:Animals; D001651:Cholestasis, Extrahepatic / Q000209:etiology / Q000276:immunology* / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis; D006498:Hepatectomy; D015850:Interleukin-6 / Q000096:biosynthesis / Q000097:blood*; D008026:Ligation; D008099:Liver / Q000276:immunology* / Q000378:metabolism*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D011183:Postoperative Complications / Q000276:immunology / Q000378:metabolism
D018840:HSP70 Heat-Shock Proteins; D015850:Interleukin-6