Molecular heterogeneity of bla(VIM-2)-containing integrons from Pseudomonas aeruginosa plasmids encoding the VIM-2 metallo-beta-lactamase.
A bla(VIM-2) metallo-beta-lactamase determinant, identical to that previously identified in Pseudomonas aeruginosa COL-1 isolate from a French hospital, was detected on a 28-kb plasmid carried by a nosocomial isolate of P. aeruginosa from Verona, Italy. In this plasmid the bla(VIM-2) determinant was inserted into a class 1 integron of original structure, named In72, that contains a partially deleted intI1 integrase gene and two gene cassettes. The first cassette carries an aacA4 aminoglycoside acetyl transferase determinant. The second cassette carries a bla(VIM-2) determinant followed by a partially deleted attC site. The structure of In72 was notably different from that of In56, the bla(VIM-2)-containing integron found in the COL-1 isolate, revealing the existence of molecular heterogeneity among bla(VIM-2)-containing integrons in clinical isolates of P. aeruginosa from Europe.
Pallecchi
L
L
Dipartimento di Biologia Moleculare, Sezione di Microbiologia, Università di Sienna, Italy.
Riccio
M L
ML
Docquier
J D
JD
Fontana
R
R
Rossolini
G M
GM
D000123:Acetyltransferases / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D004251:DNA Transposable Elements; D019426:Integrases / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D011995:Recombination, Genetic; D001618:beta-Lactamases / Q000235:genetics*
D004251:DNA Transposable Elements; D000123:Acetyltransferases; C019681:aminoglycoside acetyltransferase; D019426:Integrases; C115006:integron integrase IntI1; C425108:beta-lactamase bla(vim-2); D001618:beta-Lactamases