title: Bovine herpesvirus 1 glycoprotein G is required for prevention of apoptosis and efficient viral growth in rabbit kidney cells.
abstract: In rabbit kidney (RK13) cells, gG-negative BHV-1 exhibited significant defects in plaque formation and growth compared to that of gG-positive BHV-1. RK13 cells infected with gG-negative BHV-1 exhibited a distinctive CPE and contained a larger number of cells stained with trypan blue dye compared to those infected with gG-positive strains, suggesting that gG-negative BHV-1 inflicted more damage to the infected cells than gG-positive BHV-1. Apoptotic cell death was induced in RK13 cells infected with gG-negative BHV-1 within 8 h. In contrast, the onset of apoptosis in gG-positive BHV-1-infected RK13 cells was around 12-16 h postinfection. In the presence of caspase inhibitor Z-Asp-CH2-DCB, multiplication of gG-negative minus BHV-1 was significantly increased. These results demonstrate that BHV-1 gG is involved in stabilizing the cell structure, postponing apoptotic process, and efficient BHV-1 replication in infected RK13 cells.
authors.affiliation: Department of Global Agricultural Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Tokyo, Bunkyo-ku, 113-8657, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nakamichi
authors.forename: D
authors.initials: D
authors.lastname: Kuroki
authors.forename: Y
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: H
authors.initials: H
authors.lastname: Otsuka
mesh_terms: D000818:Animals; D017209:Apoptosis; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D061945:Caspase Inhibitors; D002417:Cattle; D002460:Cell Line; D007242:Herpesvirus 1, Bovine / Q000502:physiology*; D007668:Kidney / Q000473:pathology / Q000821:virology; D009154:Mutation; D011480:Protease Inhibitors / Q000494:pharmacology; D011817:Rabbits; D013997:Time Factors; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000502:physiology*
chemical_list: D061945:Caspase Inhibitors; D011480:Protease Inhibitors; D014764:Viral Proteins; C093260:benzyloxycarbonyl-Asp-CH2OC(O)-2,6-dichlorobenzene; C046998:bovine herpesvirus type-1 glycoproteins; D001224:Aspartic Acid