Neuroprotective constituents from Hedyotis diffusa.
In a bioassay-guided search for neuroprotective compounds from medicinal plants, a MeOH extract of whole plants of Hedoytis diffusa yielded five flavonol glycosides, kaempferol 3-O-[2-O-(6-O-E-feruloyl)-beta-D-glucopyranosyl]-beta-D-galactopyranoside (1), quercetin 3-O-[2-O-(6-O-E-feruloyl)-beta-D-glucopyranosyl]-beta-D-galactopyranoside (2), quercetin 3-O-[2-O-(6-O-E-feruloyl)-beta-D-glucopyranosyl]-beta-D-glucopyranoside (3), kaempferol 3-O-(2-O-beta-D-glucopyranosyl)-beta-D-galactopyranoside (4), and quercetin 3-O-(2-O-beta-D-glucopyranosyl)-beta-D-galactopyranoside (5), and four O-acylated iridoid glycosides (6-9). Compounds 1 and 2 are previously unreported natural products, and all nine compounds exhibited significant neuroprotective activity in primary cultures of rat cortical cells damaged by L-glutamate.
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College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 151-742, Korea.
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D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D044949:Kaempferols; D009682:Magnetic Resonance Spectroscopy; D009474:Neurons / Q000187:drug effects; D018696:Neuroprotective Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry*; D011794:Quercetin / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D019666:Rubiaceae / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013056:Spectrophotometry, Ultraviolet
D005419:Flavonoids; D006027:Glycosides; D044949:Kaempferols; D018696:Neuroprotective Agents; C006552:kaempferol; D011794:Quercetin