title: Improved determination of urinary oxalate with alkylamine glass bound barley oxalate oxidase.
abstract: The measurement of oxalate in urine was improved by employing barley oxalate oxidase immobilized on alkylamine glass beads affixed in a glass beaker. The minimum detection limit was 3.6 mg l(-1) urine. The recovery of added oxalate was 88.9+/-9.2%. Within- and between-assay coefficients of variation (CV) were <4.0 and <9.4%, respectively. The urinary oxalate values were obtained by a commercial kit method and the present method showed a good correlation (0.999). The method is free from tedious handling of glass beads and Cl- interference.
authors.affiliation: Biochemistry Research Laboratory, Department of Bio-Sciences, M.D. University, Rohtak-124001, Haryana, India.
authors.forename: P
authors.initials: P
authors.lastname: Chandran
authors.forename: M
authors.initials: M
authors.lastname: Thakur
authors.forename: C S
authors.initials: CS
authors.lastname: Pundir
mesh_terms: D000328:Adult; D000588:Amines / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry; D005898:Glass; D001467:Hordeum / Q000201:enzymology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D008863:Microspheres; D010070:Oxalates / Q000652:urine*; D010088:Oxidoreductases / Q000032:analysis*; D010940:Plant Proteins / Q000737:chemistry; D013696:Temperature; D013997:Time Factors
chemical_list: D000588:Amines; D004800:Enzymes, Immobilized; D010070:Oxalates; D010940:Plant Proteins; D010088:Oxidoreductases; C020598:oxalate oxidase