title: Influence of protein composition on total urinary protein determined by pyrocatechol-violet (UPRO vitros) and pyrogallol red dye binding methods.
abstract: Influence of protein composition on total urinary protein assays was evaluated for pyrogallol red-molybdate both with and without sodium dodecyl sulfate (SDS) and pyrocatechol violet-molybdate complex (UPRO) techniques. Using mixtures of albumin and gamma-globulins (n = 8; albumin/globulin ratio, 0 to 10), mean recoveries were 79, 77, and 81% for pyrogallol red, pyrogallol red-SDS, and UPRO, respectively. Using diluted myeloma sera (n = 26; A/G ratio, 0.39 to 2.35), mean recovery by the UPRO method was 115% (vs. 63% for pyrogallol red and 83% pyrogallol red-SDS). Results positively correlated with A/G ratio for UPRO (r = 0.69; P < 0.001), pyrogallol red (r = 0.48; P < 0.05), but not pyrogallol red-SDS (r = 0.191; NS). The difference between UPRO and pyrogallol red assays correlated with the A/G ratio (r = 0.82; P < 0.001). In light chain proteinuria (n = 10), no significant difference (< 15%) was observed between techniques, whereas in glomerular selective proteinuria (n = 10), values were significantly higher with the UPRO assay (2.20 +/- 1.61 vs. 1.43 +/- 1.10 g/L; P < 0.02). Our results support the idea that screening for renal diseases can be performed with UPRO or pyrogallol red assays. However, since A/G ratios may vary with renal disease evolution, follow-up of patients with positive proteinuria should be performed using the same assay, preferably the pyrogallol red-SDS.
authors.affiliation: Service de Biochimie, Hôpital Tenon, Assistance Publique, 4 rue de la Chine, Paris, France. guillame.lefevre@tnn.ap-hop-paris.fr
authors.forename: G
authors.initials: G
authors.lastname: Lefèvre
authors.forename: S
authors.initials: S
authors.lastname: Bloch
authors.forename: T
authors.initials: T
authors.lastname: Le Bricon
authors.forename: S
authors.initials: S
authors.lastname: Billier
authors.forename: S
authors.initials: S
authors.lastname: Arien
authors.forename: J
authors.initials: J
authors.lastname: Capeau
mesh_terms: D001536:Bence Jones Protein / Q000652:urine; D001557:Benzenesulfonates; D004396:Coloring Agents; D006801:Humans; D008982:Molybdenum; D009101:Multiple Myeloma / Q000097:blood; D011507:Proteinuria / Q000652:urine*; D011748:Pyrogallol / Q000031:analogs & derivatives*; D012680:Sensitivity and Specificity; D012709:Serum Albumin / Q000032:analysis; D012710:Serum Albumin, Bovine / Q000032:analysis; D012967:Sodium Dodecyl Sulfate; D012996:Solutions; D005719:gamma-Globulins / Q000032:analysis
chemical_list: D001557:Benzenesulfonates; D004396:Coloring Agents; D012709:Serum Albumin; D012996:Solutions; D005719:gamma-Globulins; D011748:Pyrogallol; C009134:pyrocatechol violet; C044659:molybdate; D012710:Serum Albumin, Bovine; C058731:pyrogallol sulfonphthalein; D012967:Sodium Dodecyl Sulfate; D008982:Molybdenum; D001536:Bence Jones Protein