Constitutive expression of the 27-kDa heat-shock protein in neurons and satellite cells in the peripheral nervous system of the rat.
By use of reverse transcriptase-polymerase chain reaction, abundant expression of the mRNA of 27 kDa heat shock protein (Hsp27) was revealed in the sympathetic and parasympathetic ganglia as well as in the sensory ganglia of unstressed adult rats. In situ hybridization and immunohistochemistry further localized Hsp27 mRNA and protein to both neurons and satellite cells in all types of ganglia examined. Schwann cells in the ganglia and peripheral nerve fibers were devoid of Hsp27 signal. These results suggested that Hsp27 is constitutively expressed in neurons and satellite cells in the entire peripheral nervous system of the rat.
Yamamoto
M
M
Department of Anatomy, School of Medicine, Kanazawa University, Kanazawa, Japan. miyukiy@basic.m.kanazawa-u.ac.jp
Fan
L
L
Wakayama
T
T
Amano
O
O
Iseki
S
S
D000818:Animals; D005726:Ganglia, Parasympathetic / Q000378:metabolism / Q000648:ultrastructure; D017950:Ganglia, Sensory / Q000378:metabolism / Q000648:ultrastructure; D005728:Ganglia, Sympathetic / Q000378:metabolism / Q000648:ultrastructure; D055551:HSP27 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D009457:Neuroglia / Q000378:metabolism* / Q000648:ultrastructure; D009474:Neurons / Q000378:metabolism* / Q000648:ultrastructure; D017933:Peripheral Nervous System / Q000378:metabolism* / Q000648:ultrastructure; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D055551:HSP27 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C486114:Hspb1 protein, rat; D009363:Neoplasm Proteins; D012333:RNA, Messenger