title: Developmental expression of vascular endothelial growth factor in the masseter muscle of rats.
abstract: Developmental changes in vascular endothelial growth factor (VEGF) in rat masseter after birth were investigated. VEGF was extracted efficiently and reproducibly from muscle homogenate with low concentrations of non-ionic detergents, such as Triton X-100, Nonidet P-40, and Tween 20. The amount of VEGF measured by enzyme-linked immunosorbent assay (ELISA) increased markedly by approximately 9-fold, from day 8 to 35 after birth. The increase in VEGF was closely correlated with the development of the capillary network, as shown by the capillary to muscle fibre ratio (C/F ratio). Immunoblotting revealed that the predominant molecular species of VEGF concentrated with heparin-sepharose beads was VEGF(188). These results suggest that VEGF plays an important part in the development and maintenance of the capillary network in the rat masseter.
authors.affiliation: Department of Oral Physiology, School of Dentistry, Health Sciences University of Hokkaido, Tobetsu, Hokkaido 061-0293, Japan. hisayosh@hoku-iryo-u.ac.jp
authors.forename: H
authors.initials: H
authors.lastname: Ishii
authors.forename: I
authors.initials: I
authors.lastname: Oota
authors.forename: T
authors.initials: T
authors.lastname: Takuma
authors.forename: K
authors.initials: K
authors.lastname: Inomata
mesh_terms: D000818:Animals; D002196:Capillaries / Q000254:growth & development*; D003902:Detergents; D016228:Endothelial Growth Factors / Q000096:biosynthesis* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D018507:Gene Expression Regulation, Developmental; D006651:Histocytochemistry; D015151:Immunoblotting; D008222:Lymphokines / Q000096:biosynthesis* / Q000302:isolation & purification; D008406:Masseter Muscle / Q000098:blood supply* / Q000378:metabolism; D017830:Octoxynol; D051381:Rats; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
chemical_list: D003902:Detergents; D016228:Endothelial Growth Factors; D008222:Lymphokines; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D017830:Octoxynol