title: Contribution of mitochondria and peroxisomes to palmitate oxidation in pea tissues.
abstract: Total, mitochondrial and peroxisomal palmitate oxidation capacities were compared in pea, from the dry seed to 14 days after imbibition. Total beta-oxidation varied over the measured time period and showed four peaks of activity at day 2, days 5-6, day 10 and days 12-13. The contribution of peroxisomal and mitochondrial beta-oxidation to this overall beta-oxidation varied. Over the first 48 h of seed germination, peroxisomal beta-oxidation accounted for 80-100% of the total observed beta-oxidation. The larger peaks of beta-oxidation at days 5-6, day 10 and days 12-13 were due primarily to mitochondrial beta-oxidation activity, which accounted for 70-90% of the observed total beta-oxidation at these times. The peaks of activity are related to observed stages in seedling development.
authors.affiliation: Department of Biological and Nutritional Sciences, Newcastle University, Newcastle upon Tyne NE1 7RU, UK. christine.masterson@ncl.ac.uk
authors.forename: C
authors.initials: C
authors.lastname: Masterson
authors.forename: C
authors.initials: C
authors.lastname: Wood
mesh_terms: D002245:Carbon Dioxide / Q000378:metabolism; D002250:Carbon Radioisotopes; D007700:Kinetics; D008928:Mitochondria / Q000378:metabolism*; D010084:Oxidation-Reduction; D019308:Palmitic Acid / Q000378:metabolism*; D018532:Pisum sativum / Q000254:growth & development / Q000378:metabolism*; D020675:Peroxisomes / Q000378:metabolism*; D013997:Time Factors
chemical_list: D002250:Carbon Radioisotopes; D002245:Carbon Dioxide; D019308:Palmitic Acid