title: Cytogenetic analysis of cryopreserved bone marrow cells.
abstract: We studied the results of culturing and karyotyping of fresh BM and defrosted BM cells from 11 patients with cytogenetic abnormalities with the diagnosis of acute myeloid leukemia (AML). In 8 of 11 patients the cytogenetic results from the fresh and defrosted BM were comparable with respect to the amount and quality of abnormal metaphases. In only one patient one abnormal cell-line (out of two) re-appeared in the frozen BM, probably due to selection, and in three patients we found a distinct decrease of amount and quality. In conclusion, cytogenetic analysis of cryopreserved BM cells can be a reliable alternative to analysis of fresh cells.
authors.affiliation: Department of Medical Genetics, University of Groningen, Ant. Deusinglaan 4, 9713 AW, Groningen, The Netherlands. a.g.bosga-bouwer@medgen.azg.nl
authors.forename: A G
authors.initials: AG
authors.lastname: Bosga-Bouwer
authors.forename: D
authors.initials: D
authors.lastname: Hendriks
authors.forename: E
authors.initials: E
authors.lastname: Vellenga
authors.forename: H
authors.initials: H
authors.lastname: Zorgdrager
authors.forename: E
authors.initials: E
authors.lastname: van den Berg
mesh_terms: D001854:Bone Marrow Cells / Q000502:physiology*; D015925:Cryopreservation / Q000379:methods*; D003582:Cytogenetics / Q000379:methods*; D006801:Humans; D007621:Karyotyping; D007951:Leukemia, Myeloid / Q000235:genetics; D008677:Metaphase / Q000235:genetics