title: [Cloning and endonuclease digestion of lon gene from Streptomyces ansochromogenes].
abstract: Streptomyces ansochromogenes is a nikkomycin producer which has typical life cycle of Streptomyces differentiation on glucose asparagine medium. The lon gene product is an ATP-dependent serine proteinase. The lon gene might have some connection with physiological differentiation and also with the morphological differentiation in S. coelicolor. The lon-like gene of S. ansochromogenes was identified by hybridization and the lon-like gene in 4.6 kb PstI fragment was cloned. The physical map of this fragment and the localization of the target gene were preliminarily studied.
authors.affiliation: Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080.
authors.forename: G
authors.initials: G
authors.lastname: Liu
authors.forename: H
authors.initials: H
authors.lastname: Tan
mesh_terms: D003001:Cloning, Molecular; D005798:Genes, Bacterial; D010957:Plasmids; D013302:Streptomyces / Q000235:genetics*