Rapid coliphage detection assay.
A rapid coliphage detection assay was developed, based on the phage-induced release of beta-galactosidase from cells of Escherichia coli. The assay could detect as few as five coliphage per sample without an overnight incubation period. The range of acceptable assay parameters was identified.
Stanek
J E
JE
Department of Biology, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061-0406, USA.
Falkinham
J O
JO
D003090:Coliphages / Q000302:isolation & purification*; D004926:Escherichia coli / Q000201:enzymology; D012680:Sensitivity and Specificity; D013997:Time Factors; D014871:Water Microbiology; D001616:beta-Galactosidase / Q000096:biosynthesis
D001616:beta-Galactosidase