Stimulation of Hyaluronan synthetase by platelet-derived growth factor bb in human prostate smooth muscle cells.
Hyaluronic acid (HA) is an important component of the extracellular matrix of prostate cells. Platelet-derived growth factor bb (PDGFbb) has a mitogenic effect on prostate stromal cells. The effect of PDGFbb on HA production by smooth muscle cells from normal and benign prostatic hyperplasia (BPH) tissue was studied to elucidate the role of these two factors in BPH. The basal level of HA released into the cell media was greater in BPH cells. PDGFbb increases HA production in normal and BPH smooth muscle cells. This is partly due to an increase in HA synthetase II mRNA expression. In prostate disease, PDGFbb may have a role that involves HA production.
Pullen
M
M
Department of Renal Pharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia, PA, USA. Mark_A_Pullen@sbphrd.com
Thomas
K
K
Wu
H
H
Nambi
P
P
D000925:Anticoagulants / Q000494:pharmacology*; D000077214:Becaplermin; D002478:Cells, Cultured; D014453:Glucuronosyltransferase / Q000187:drug effects* / Q000378:metabolism; D016695:Glycosyltransferases; D006801:Humans; D000076002:Hyaluronan Synthases; D008297:Male; D008565:Membrane Proteins; D009130:Muscle, Smooth / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D011467:Prostate / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D011470:Prostatic Hyperplasia / Q000201:enzymology*; D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D014166:Transferases; D029867:Xenopus Proteins
D000925:Anticoagulants; D008565:Membrane Proteins; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger; D029867:Xenopus Proteins; D000077214:Becaplermin; D014166:Transferases; D016695:Glycosyltransferases; D014453:Glucuronosyltransferase; C056944:HAS1 protein, Xenopus; C101754:HAS2 protein, human; C491096:Has2 protein, Xenopus; D000076002:Hyaluronan Synthases