title: Miniaturized tests for computer-assisted identification of motile Aeromonas species with an improved probability matrix.
abstract: OBJECTIVE
  To develop miniaturized tests for the phenotypic identification of motile Aeromonas species using an improved probability matrix.
  
  
  RESULTS
  Conventional tests were miniaturized for use in 96-well plates, and their performance assessed using 60 aeromonads comprising type and reference strains as well as clinical, fish and water isolates. A revised probability matrix for Aeromonas hybridization groups 1-14, including A. allosaccharophila, A. bestiarum, A. encheleia and A. popoffii, was developed. Using 26 tests, all the reference strains were correctly identified with the revised probability matrix, and 80% of the isolates were correctly identified at a Willcox probability level of 95%.
  
  
  CONCLUSIONS
  The compact test format, coupled with a robust identification matrix, provides a convenient basis for identifying motile aeromonads.
  
  
  CONCLUSIONS
  The identification system for identifying aeromonads will be of use to medical and veterinary laboratories undertaking disease diagnosis.
authors.affiliation: Fish Health Unit, Department of Primary Industries, Water & Environment, PO Box 46, Kings Meadows, Launceton, Tasmania 7249, Australia. jeremy.carson@dpiwe.tas.gov.au
authors.forename: J
authors.initials: J
authors.lastname: Carson
authors.forename: T
authors.initials: T
authors.lastname: Wagner
authors.forename: T
authors.initials: T
authors.lastname: Wilson
authors.forename: L
authors.initials: L
authors.lastname: Donachie
mesh_terms: D000333:Aeromonas / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology; D000818:Animals; D015373:Bacterial Typing Techniques; D003198:Computer Simulation; D003470:Culture Media; D005399:Fishes / Q000382:microbiology; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D006801:Humans; D008962:Models, Theoretical; D009068:Movement / Q000502:physiology; D011336:Probability; D015203:Reproducibility of Results
chemical_list: D003470:Culture Media