Bowman-Birk protease inhibitor and its palmitic acid conjugate prevent 7,12-dimethylbenz[a]anthracene-induced transformation in cultured mouse mammary glands.
The chemopreventive effects of Bowman-Birk protease inhibitor (BBI), a soybean polypeptide, and its palmitic acid conjugate, Pal-BBI, on 7,12-dimethylbenz[a]anthracene (DMBA)-induced transformation were investigated by using an in vitro whole organ culture system of mouse mammary glands. The transformation incidence was measured as the number of glands containing nodule-like alveolar lesions (NLAL). Both BBI and Pal-BBI were effective in preventing DMBA-induced transformation, especially when added to the medium during the developing period after the exposure of mammary glands to DMBA, i.e. 35.9 and 53.4% prevention, respectively. However, when only present in the medium before the promotion period, Pal-BBI was effective in decreasing the transformation incidence (32.2%) while BBI was not (10.3%). This difference could possibly be due to the high lipophilicity and tissue retention of Pal-BBI in mammary gland cultures.
Du
X
X
Department of Pharmaceutical Sciences, School of Pharmacy, University of Southern California, 1985 Zonal Avenue, Los Angeles, CA 90089-9121, USA.
Beloussow
K
K
Shen
W C
WC
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D001940:Breast / Q000166:cytology*; D002273:Carcinogens; D002478:Cells, Cultured; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D009369:Neoplasms / Q000517:prevention & control; D009924:Organ Culture Techniques; D019308:Palmitic Acid / Q000494:pharmacology*; D011650:Pulmonary Alveoli / Q000378:metabolism; D014358:Trypsin Inhibitor, Bowman-Birk Soybean / Q000494:pharmacology*
D002273:Carcinogens; D014358:Trypsin Inhibitor, Bowman-Birk Soybean; D019308:Palmitic Acid; D015127:9,10-Dimethyl-1,2-benzanthracene