title: [Enhancement of Vp16 inducing apoptosis of leukemic cells by retrovirus-mediated bcl-2 anti-sense RNA].
abstract: OBJECTIVE
  To explore the effects of retrovirus-mediated bcl-2 antisense RNA on apoptosis of leukemic cells.
  
  
  METHODS
  Retrovirus was packaged in vitro with PA317 cells and Jurkat cell was transducted with collected virus; The expressions of bcl-2 mRNA and protein were assayed by RT-PCR and Western blotting, respectively. Apoptosis was assayed by flow cytometry and DNA "ladder".
  
  
  RESULTS
  Expression of intrinsic bcl-2 was decreased, and the sensitivity of leukemic cells to Vp16 and the apoptosis of leukemic cells line Jurkat cells were enhanced by transfected bcl-2 antisense RNA.
  
  
  CONCLUSIONS
  Antisense bcl-2 enhances Vp16 inducing apoptosis of leukemic cells. The results provide a useful experimental basis for leukemia therapy.
authors.affiliation: Central Laboratory, People's Hospital, Beijing Medical University, Beijing 100044.
authors.forename: G
authors.initials: G
authors.lastname: Cao
authors.forename: S
authors.initials: S
authors.lastname: Wang
authors.forename: D
authors.initials: D
authors.lastname: Chen
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D005047:Etoposide / Q000494:pharmacology*; D019254:Genes, bcl-2; D006801:Humans; D019169:Jurkat Cells; D012190:Retroviridae / Q000235:genetics
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D005047:Etoposide