Prolactin activation of IRF-1 transcription involves changes in histone acetylation.
In response to prolactin (PRL) signaling, transcription of the interferon regulatory factor-1 gene (IRF-1) is rapidly induced during early G(1), declines in mid G(1), and rises again over the G(1)/S transition phase of the cell cycle in Nb2 T cells. Using chromatin immunoprecipitation assays, we show that histone H4 acetylation increases over a 1.7 kb region of the IRF-1 promoter in early G(1) and again at the G(1)/S transition in response to PRL stimulation. These results demonstrate a correlation between histone H4 hyperacetylation at the IRF-1 promoter and biphasic transcription of IRF-1 in response to PRL signaling in vivo.
Book McAlexander
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Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA.
Yu-Lee
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L
D000107:Acetylation / Q000187:drug effects; D000123:Acetyltransferases / Q000378:metabolism; D000818:Animals; D002843:Chromatin / Q000737:chemistry / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics*; D016193:G1 Phase / Q000187:drug effects; D051548:Histone Acetyltransferases; D006657:Histones / Q000737:chemistry* / Q000378:metabolism*; D050856:Interferon Regulatory Factor-1; D010750:Phosphoproteins / Q000235:genetics*; D011233:Precipitin Tests; D011388:Prolactin / Q000494:pharmacology*; D011401:Promoter Regions, Genetic / Q000235:genetics; D051381:Rats; D016196:S Phase / Q000187:drug effects; D029701:Saccharomyces cerevisiae Proteins; D015398:Signal Transduction / Q000187:drug effects; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects; D015533:Transcriptional Activation / Q000187:drug effects*; D014407:Tumor Cells, Cultured
D002843:Chromatin; D004268:DNA-Binding Proteins; D006657:Histones; D050856:Interferon Regulatory Factor-1; C494259:Irf1 protein, rat; D010750:Phosphoproteins; D029701:Saccharomyces cerevisiae Proteins; D011388:Prolactin; D000123:Acetyltransferases; D051548:Histone Acetyltransferases