Evidence of cross-reactivity between porcine pancreatic and rapeseed (Brassica napus L.) lipases.
A cross-reactivity between animal and plant lipases was determined, using immunological techniques. It was shown by ELISA and dot-blotting that these antibodies react with lipases in the rapeseed crude extract and in the different cellular fractions obtained by differential centrifugation. Pre-incubation of the antiserum with the rapeseed crude extract affects the amount of antibodies binding to the porcine pancreatic lipase. Antibodies were able to precipitate lipase activity from 3-day-old rapeseed crude extract. These epitopes seem to be located in the catalytic site, suggesting that a consensus sequence exists in oleaginous lipases and that it will be universal.
Belguith
H
H
Departement des Sciences Biologiques, Laboratoire de Biochimie des Protéines, Faculté des Sciences de Tunis, 1060 Tunis, Tunisia. belguith@excite.com
Jridi
T
T
Ben Hamida
J
J
D000818:Animals; D000937:Antigen-Antibody Reactions; D001937:Brassica / Q000276:immunology*; D018548:Cotyledon / Q000201:enzymology; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D007700:Kinetics; D008049:Lipase / Q000276:immunology*; D010179:Pancreas / Q000201:enzymology*; D013552:Swine
D008049:Lipase