Effect of genetic cross on the detection of quantitative trait loci and a novel approach to mapping QTLs.
A genome-wide scan was conducted in two F(2) intercrosses, C57BL/6J (B6)xDBA/2J (D2) and BALB/cJ (C)xLP/J (LP), for three different phenotypes: basal locomotor activity, ethanol-induced locomotor activity, and haloperidol-induced catalepsy. For basal activity, significant quantitative trait loci (QTLs, LOD> or =4.3) were detected on chromosomes 9 and 19 for the CxLP intercross and chromosome 1 for the B6xD2 intercross. Significant QTLs for ethanol-induced activation were detected on chromosome 6 for the CxLP intercross, and on chromosomes 1 and 2 for the B6xD2 intercross. For haloperidol-induced catalepsy, significant QTLs were detected on chromosome 14 (two different QTLs) in the CxLP intercross, and chromosomes 1 and 9 in the B6xD2 intercross. These data illustrate the importance of the genetic cross for QTL detection. Finally, the data reported here, and elsewhere, are also used to demonstrate a novel approach to QTL detection and localization.
Hitzemann
R
R
Departments of Psychiatry, Psychology, and Neuroscience, SUNY at Stony Brook, Stony Brook, NY 11794-8101, USA.
Demarest
K
K
Koyner
J
J
Cipp
L
L
Patel
N
N
Rasmussen
E
E
McCaughran
J
J
D000818:Animals; D002375:Catalepsy / Q000139:chemically induced / Q000235:genetics*; D002492:Central Nervous System Depressants / Q000494:pharmacology; D003433:Crosses, Genetic; D018492:Dopamine Antagonists / Q000009:adverse effects; D000431:Ethanol / Q000494:pharmacology; D005260:Female; D006220:Haloperidol / Q000009:adverse effects; D008126:Lod Score; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009043:Motor Activity / Q000187:drug effects / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*; D019655:Quantitative Trait, Heritable
D002492:Central Nervous System Depressants; D018492:Dopamine Antagonists; D000431:Ethanol; D006220:Haloperidol