Syk protein-tyrosine kinase is involved in neuron-like differentiation of embryonal carcinoma P19 cells.
Syk has been implicated in activated immunoreceptors to downstream signaling events in hematopoietic cells. Here we report that Syk is expressed in neuron-like cells and involved in neuron-like differentiation of embryonal carcinoma P19 cells. Immunoblot, RT-PCR, and Northern analysis indicated that Syk is expressed in mouse brain, PC12 and P19 cells. In addition, Syk was found to be tyrosine phosphorylated during neuron-like differentiation of P19 cells. Furthermore, adenovirus-mediated overexpression of Syk induced supernumerary neurite formation and extracellular signal-regulated kinase (ERK) activation in P19 cells. These results suggest that Syk plays an important role in signaling steps leading to ERK activation in P19 cells.
Tsujimura
T
T
Department of Biochemistry, Kobe University School of Medicine, Chuo-ku, 650-0017, Kobe, Japan.
Yanagi
S
S
Inatome
R
R
Takano
T
T
Ishihara
I
I
Mitsui
N
N
Takahashi
S
S
Yamamura
H
H
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D001921:Brain / Q000166:cytology / Q000201:enzymology; D018236:Carcinoma, Embryonal / Q000201:enzymology* / Q000473:pathology; D002454:Cell Differentiation; D004274:DNA, Recombinant; D004789:Enzyme Activation; D004792:Enzyme Precursors / Q000235:genetics / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D016501:Neurites / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000201:enzymology; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000072377:Syk Kinase; D014407:Tumor Cells, Cultured; D014443:Tyrosine / Q000378:metabolism
D004274:DNA, Recombinant; D004792:Enzyme Precursors; D047908:Intracellular Signaling Peptides and Proteins; D012333:RNA, Messenger; D014443:Tyrosine; D011505:Protein-Tyrosine Kinases; D000072377:Syk Kinase; C000606897:Syk protein, mouse; D020928:Mitogen-Activated Protein Kinases