Detection of Salmonella and simultaneous detection of Salmonella and Shiga-like toxin-producing Escherichia coli using the magnetic capture hybridization polymerase chain reaction.
The magnetic capture hybridization polymerase chain reaction (MCH-PCR) was used to detect Salmonella and also to simultaneously detect Salmonella and Shiga-like toxin-producing Escherichia coli (SLTEC). Fifty-seven Salmonella and 41 SLTEC were included in the study. Salmonella were detected either individually by a single MCH-PCR assay targeting the inv gene or simultaneously with SLTEC by a multiplex MCH-PCR in which SLTEC were detected using primers for the slt genes. Both single and multiplex assays were found to be specific for tested pathogens. The results indicate that MCH-PCR can be used as means of detecting single or multiple bacterial pathogen(s).
Chen
J
J
Center for Food Safety and Quality Enhancement, Department of Food Science and Technology, The University of Georgia, Griffin, GA 30223-1797, USA. jchen@cfsqe.griffin.peachnet.edu
Griffiths
M W
MW
D018829:Adhesins, Bacterial; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D001710:Biotin; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D012475:Salmonella / Q000235:genetics / Q000302:isolation & purification*; D022603:Shiga Toxins / Q000235:genetics / Q000378:metabolism*
D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D015342:DNA Probes; D004269:DNA, Bacterial; D022603:Shiga Toxins; C053476:invasin, Yersinia; D001710:Biotin