Dietary oxidized oil influences the levels of type 2 T-helper cell-related antibody and inflammatory mediators in mice.
The aim of this present study was to investigate the effect of amount and degree of oxidation of dietary oil on type 2 T-helper cell (TH)-related immune responses. Four groups of BALB/c mice were fed either 50 g soyabean oil/kg (50-S), 50 g oxidized oil/kg (50-O), 150 g soyabean oil/kg (150-S) or 150 g oxidized oil/kg (150-O). After 14 weeks consuming the experimental diets, the mice were immunized with ovalbumin (OVA) plus Al and antigen-specific immunoglobulin (Ig)E, IgG1 and IgG2a, inflammatory mediators such as prostaglandin (PG) E2 and leukotriene (LT)B4 were determined. Higher hepatic microsomal cytochrome P450 was noted in mice fed 150 g oxidized oil/kg compared with those of other groups. OVA-specific IgG1 and IgE were higher in mice fed 150 g oxidized oil/kg compared with those of the other groups. The data suggested the interleukin (IL)-4: interferon (IFN)-gamma ratio was higher in mice fed 50 g dietary oxidized oil/kg compared with that of the 50-S group. The IL-5:IFN-gamma ratios were higher in the 150-S and 150-O groups than in the 50-S and 50-O groups. PGE2 and LTB4 produced by macrophages stimulated by lipopolysaccharide were highest in mice in the 150 g oxidized oil/kg group. The data suggested that an increased intake of oxidized oil might exert an unfavourable effect on the TH2 response involved in allergic disease.
Lin
B F
BF
Laboratory of Nutritional Chemistry, Department of Agricultural Chemistry, College of Agriculture, National Taiwan University, Taipei, Taiwan 10764, Republic of China.
Lai
C C
CC
Lin
K W
KW
Chiang
B L
BL
D000818:Animals; D001249:Asthma / Q000209:etiology; D002455:Cell Division / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis; D015232:Dinoprostone / Q000096:biosynthesis; D002149:Energy Intake; D005260:Female; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000096:biosynthesis; D018836:Inflammation Mediators / Q000378:metabolism*; D007975:Leukotriene B4 / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D010084:Oxidation-Reduction; D013024:Soybean Oil / Q000737:chemistry / Q000494:pharmacology*; D013154:Spleen / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000187:drug effects* / Q000276:immunology; D015430:Weight Gain / Q000187:drug effects
D016207:Cytokines; D007074:Immunoglobulin G; D018836:Inflammation Mediators; D007975:Leukotriene B4; D007073:Immunoglobulin E; D013024:Soybean Oil; D010047:Ovalbumin; D015232:Dinoprostone