title: Maf genes are involved in multiple stress response in human.
abstract: The Maf protein family consists of eight transcription factors containing a basic-leucine zipper (bZIP) domain. We have previously reported that the mRNA to one of these members, mafG/adapt66, is induced by oxidative stress in hamster HA-1 cells. It has subsequently been reported that mafG is induced bystress that activates the expression of genes under the control of the antioxidant/electrophile response element (ARE/EpRE), and that small Maf proteins are present in ARE/EpRE-protein complexes. Here we extend these studies to assess the effects of various types of stress on maf mRNA induction in human cells. The oxidative stressor cadmium, and the heavy metals cadmium, zinc, and arsenite induced mafG RNA levels within two hours, and maximally at five hours for cadmium and zinc. This induction was observed for multiple transcripts including two not normally associated with mafG, suggesting that these stress agents induced the expression of other related maf family RNAs. Modest induction of mafG mRNA was also observed with heat shock but not calcium elevation. These results suggest that mafG is a human stress-response gene induced by multiple stress, and that several maf (proto-)oncogene members play an important role in cellular stress response.
authors.affiliation: Department of Medicine, Lady Davis Institute for Medical Research, McGill University, 3755 Cote Ste-Catherine Road, Montreal, Quebec, H3T 1E2, Canada.
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
authors.forename: V
authors.initials: V
authors.lastname: Blank
authors.forename: J S
authors.initials: JS
authors.lastname: Sesay
authors.forename: D R
authors.initials: DR
authors.lastname: Crawford
mesh_terms: D000818:Animals; D018053:Arsenites / Q000494:pharmacology; D019256:Cadmium Chloride / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002712:Chlorides / Q000494:pharmacology; D006224:Cricetinae; D004268:DNA-Binding Proteins / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D006367:HeLa Cells; D006358:Hot Temperature; D006801:Humans; D007700:Kinetics; D051259:MafG Transcription Factor; D019216:Metals, Heavy / Q000494:pharmacology; D018384:Oxidative Stress; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics*; D017670:Sodium Compounds / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects / Q000502:physiology*; D017967:Zinc Compounds / Q000494:pharmacology
chemical_list: D018053:Arsenites; D002712:Chlorides; D004268:DNA-Binding Proteins; D018840:HSP70 Heat-Shock Proteins; C103944:MAFG protein, human; C000715470:MAS1 protein, human; D051259:MafG Transcription Factor; D019216:Metals, Heavy; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger; D012097:Repressor Proteins; D017670:Sodium Compounds; D017967:Zinc Compounds; D000001:Calcimycin; C017947:sodium arsenite; C016837:zinc chloride; D019256:Cadmium Chloride