Generation of IgM anti-platelet autoantibody in dengue patients.
Dengue virus infection causes a wide range of diseases from dengue fever to life-threatening dengue hemorrhagic fever and dengue shock syndrome (DHF/DSS). The mechanisms involved in DHF/DSS pathogenesis remain unclear. Patient sera collected from an outbreak in southern Taiwan from November 1998 to January 1999 were studied. The presence of antibodies which cross-reacted with platelets could be detected in patient sera, and the isotype of these autoantibodies was IgM. The anti-platelet IgM levels were higher in DHF/DSS than in dengue fever patient sera in disease acute phase. These autoantibodies were still detectable in convalescent stage (1-3 weeks after acute phase) and even eight to nine months after illness. The platelet binding activity was not observed in other virus-infected patient sera tested. Further investigation showed that dengue patient sera caused platelet lysis in the presence of complement. The platelet cytotoxicity induced by DHF/DSS patient sera was higher than that by dengue fever sera. Dengue patient sera also inhibited platelet aggregation which, however, appeared to be not related to DHF/DSS development.
Lin
C F
CF
Department of Microbiology and Immunology, National Cheng Kung University Medical College, Tainan, Taiwan.
Lei
H Y
HY
Liu
C C
CC
Liu
H S
HS
Yeh
T M
TM
Wang
S T
ST
Yang
T I
TI
Sheu
F C
FC
Kuo
C F
CF
Lin
Y S
YS
D000208:Acute Disease; D000293:Adolescent; D001323:Autoantibodies / Q000097:blood*; D001792:Blood Platelets / Q000276:immunology*; D002648:Child; D003289:Convalescence; D003715:Dengue / Q000097:blood / Q000453:epidemiology / Q000276:immunology*; D004196:Disease Outbreaks; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood*; D008297:Male; D008875:Middle Aged; D013624:Taiwan / Q000453:epidemiology
D001323:Autoantibodies; D007075:Immunoglobulin M