title: Lipase activity in germinating sunflower seedlings.
abstract: Studying lipase in germinating sunflower seedlings, we looked for an activator of the lipolytic activity. In the presence of 1.25 mM ATP, the enzyme activity increased 2-fold. Lipid-body lipase solubilization was realized using two detergents: Tween 80 and CHAPS. Lipolytic activity was increased 10-fold in the presence of 2% (w/v) CHAPS, showing the probable 'complexity' of the enzyme. Looking for the possible lipolytic activity of the 10000 g pellet we detected the presence of the enzyme. The pellet extract was mixed, in a range of concentrations, with the oil-body fraction. The resulting lipolytic activity was 4-fold higher. These results give clues as to the subcellular distribution of lipase and its intracellular transport.
authors.affiliation: Faculté des Sciences de Tunis, Département de Biologie, Campus Universitaire 1060, Tunis, Tunisia. sellama.bahri@fst.znu.tn
authors.forename: S
authors.initials: S
authors.lastname: Bahri
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D002793:Cholic Acids; D018548:Cotyledon / Q000201:enzymology; D003902:Detergents; D006368:Helianthus / Q000201:enzymology* / Q000254:growth & development; D007700:Kinetics; D008049:Lipase / Q000302:isolation & purification / Q000378:metabolism*; D008066:Lipolysis; D015388:Organelles / Q000201:enzymology; D011136:Polysorbates
chemical_list: D002793:Cholic Acids; D003902:Detergents; D011136:Polysorbates; D000255:Adenosine Triphosphate; D008049:Lipase; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate