Rapid discrimination between BRO beta-lactamases from clinical isolates of Moraxella catarrhalis using restriction endonuclease analysis.
An important feature of Moraxella catarrhalis is the production of beta-lactamases, which causes resistance to the penicillins. Restriction enzyme analysis was able to distinguish between the bro-1 and bro-2 beta-lactamase-encoding genes from 89 clinical isolates of M. catarrhalis. This is a rapid, simple and cost effective method of characterizing these genes.
du Plessis
M
M
South African Institute for Medical Research, Pneumococcal Diseases Research Unit, de Korte St, Hillbrow, Jhb, P. O. Box 1038, 2000, Johannesburg, South Africa. mignondp@hotmail.com
D003365:Costs and Cost Analysis; D017931:DNA Primers; D004352:Drug Resistance, Microbial / Q000235:genetics; D005784:Gene Amplification; D005798:Genes, Bacterial; D001936:Moraxella catarrhalis / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping / Q000379:methods*; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
D017931:DNA Primers; D001618:beta-Lactamases