[The ex vivo expansion of cord blood hematopoietic cells: 1. Cell growth behavior].
The population growth and metabolism of hematopoietic cells from cord blood were investigated without and with hematopoietic growth factors and the long-term growth kinetics of cells was studied. In static cultivation of hematopoietic cells, the population specific growth rate was 0.34 d-1, and the average double time 2 days. The greater part of glucose was consumed and 40 mmol/L lactate was formed during cell culture. For the long-term culture, the maximum output of CFU-GM appeared between the 2nd and 3rd weeks and that of BFU-E in the first week. With 50% medium exchange, the expansions of total cells, CFU-GM, and BFU-E came up to 14, 13, and 5 times, respectively.
Zhang
Y X
YX
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237.
Ying
X F
XF
Tan
W S
WS
D002455:Cell Division; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology*; D005947:Glucose / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D007231:Infant, Newborn; D019344:Lactic Acid / Q000378:metabolism
D019344:Lactic Acid; D005947:Glucose