Convenient genotyping of six myostatin mutations causing double-muscling in cattle using a multiplex oligonucleotide ligation assay.
We herein describe a procedure that allows for simultaneous genotyping of six loss-of-function mutations in the bovine myostatin gene associated with the double-muscling phenotype. The proposed method relies on a multiplex oligonucleotide ligation assay and detection of the fluorescently labelled products using automatic sequencers.
Karim
L
L
Department of Genetics, Faculty of Veterinary Medicine, University of Liège, Belgium.
Coppieters
W
W
Grobet
L
L
Valentini
A
A
Georges
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D017931:DNA Primers; D005821:Genetic Techniques; D024510:Muscle Development; D009132:Muscles / Q000378:metabolism; D009154:Mutation; D055435:Myostatin; D009841:Oligonucleotides; D016212:Transforming Growth Factor beta / Q000235:genetics*
D017931:DNA Primers; D055435:Myostatin; D009841:Oligonucleotides; D016212:Transforming Growth Factor beta