[Molecular mechanisms of mutations induced by DNA lesions].
Oligodeoxyribonucleotides containing a DNA lesion were synthesized and inserted into vector DNAs to introduce the lesion at a predetermined site. The manipulated DNAs were transfected into living mammalian cells and the mutants induced by the DNA lesion were collected and analyzed. This approach was applied to various DNA lesions produced in cells by reactive oxygen species, chemicals, and ultraviolet light. In addition, the mutations induced by damaged DNA precursors were studied by the use of chemically synthesized nucleoside triphosphates. In this review article, the author summarizes the results obtained by these types of experiments, focusing on two oxidatively damaged compounds, 8-hydroxyguanine (7,8-dihydro-8-oxoguanine) and 2-hydroxyadenine (1,2-dihydro-2-oxoadenine).
Kamiya
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Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan.
D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D004249:DNA Damage / Q000235:genetics*; D011905:Genes, ras; D005822:Genetic Vectors; D006147:Guanine / Q000031:analogs & derivatives*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D014170:Transformation, Genetic
C024829:8-hydroxyguanine; D006147:Guanine; C008183:isoguanine