title: Anti-interleukin 8 autoantibody: interleukin 8 complexes in the acute respiratory distress syndrome. Relationship between the complexes and clinical disease activity.
abstract: Increased levels of interleukin 8 (IL-8) are found in bronchoalveolar lavage (BAL) fluids from patients with the acute respiratory distress syndrome (ARDS). However, IL-8 is not an efficient predictor of the course of ARDS. Our prior studies demonstrated that IL-8 present in lung fluids from patients with ARDS is associated with anti-IL-8 autoantibodies (anti-IL-8:IL-8 complexes). These data led us to hypothesize that the complexes might better predict the development of acute lung injury. Accordingly, we measured concentrations of free and complexed IL-8 in BAL fluids from 19 patients at risk and 45 with established ARDS on Days 1, 3, 7, 14, and 21 after the onset of ARDS. The concentrations of anti-IL-8:IL-8 complexes in patients with ARDS on Day 1 were significantly higher than in patients at risk (p < 0.05). There was a significant association between anti-IL-8:IL-8 complex concentrations and the onset of ARDS (p = 0.03). Similarly, anti-IL-8:IL-8 complex concentrations were significantly higher in patients on Day 1 of ARDS who later died (p < 0.05), and the association between high anti-IL-8: IL-8 complex concentrations and the probability of dying was significant (p = 0.03). The presence of anti-IL-8:IL-8 complexes in BAL fluids of patients with ARDS is an important prognostic indicator for the development and outcome of ARDS.
authors.affiliation: University of Texas Health Center, Tyler, Texas 75708, USA. anna.kurdowska@utht.edu
authors.forename: A
authors.initials: A
authors.lastname: Kurdowska
authors.forename: J M
authors.initials: JM
authors.lastname: Noble
authors.forename: K P
authors.initials: KP
authors.lastname: Steinberg
authors.forename: J T
authors.initials: JT
authors.lastname: Ruzinski
authors.forename: L D
authors.initials: LD
authors.lastname: Hudson
authors.forename: T R
authors.initials: TR
authors.lastname: Martin
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000936:Antigen-Antibody Complex / Q000097:blood*; D001323:Autoantibodies / Q000097:blood*; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D005260:Female; D017052:Hospital Mortality; D006801:Humans; D016209:Interleukin-8 / Q000276:immunology*; D008168:Lung / Q000276:immunology; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D012128:Respiratory Distress Syndrome / Q000175:diagnosis / Q000276:immunology* / Q000401:mortality; D015996:Survival Rate
chemical_list: D000936:Antigen-Antibody Complex; D001323:Autoantibodies; D016209:Interleukin-8