title: NMR 15N relaxation of the insulin-like growth factor (IGF)-binding domain of IGF binding protein-5 (IGFBP-5) determined free in solution and in complex with IGF-II.
abstract: 15N NMR relaxation rates of mini-IGFBP-5, an N-terminal insulin-like growth factor binding domain of the insulin-like growth factor binding protein 5 (IGFBP-5), were analysed at three field strengths using the Lipari-Szabo procedure (see below) and reduced spectral density methods. Isotropic and anisotropic Lipari-Szabo models were analysed and an analytical formula for the overall correlation time for anisotropic molecules is presented. Mini-IGFBP-5 was found to be mainly rigid on fast ps time scales except for 11 unstructured flexible residues at the C-terminus. The insulin-like growth factor binding loop in the apo-protein exhibits small amounts of flexibility on fast time scales (ps to ns) but several loop residues show significant exchange broadening. These loop residues display no exchange broadening in the complex of IGF-II/mini-IGFBP-5. The isotropic overall tumbling time in solution at 31 degrees C of mini-IGFBP-5 complexed to IGF-II is tauc = 18.4 +/- 0.2 ns indicating a strong tendency for aggregation.
authors.affiliation: Max Planck Institute for Biochemistry, Martinsried, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Renner
authors.forename: T
authors.initials: T
authors.lastname: Holak
mesh_terms: D000818:Animals; D018975:Insulin-Like Growth Factor Binding Protein 5 / Q000737:chemistry / Q000378:metabolism*; D007335:Insulin-Like Growth Factor II / Q000378:metabolism*; D046911:Macromolecular Substances; D009682:Magnetic Resonance Spectroscopy; D008962:Models, Theoretical; D009587:Nitrogen Isotopes / Q000737:chemistry; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D013002:Somatomedins / Q000378:metabolism
chemical_list: D018975:Insulin-Like Growth Factor Binding Protein 5; D046911:Macromolecular Substances; D009587:Nitrogen Isotopes; D010455:Peptides; D013002:Somatomedins; D007335:Insulin-Like Growth Factor II