Vocal cord augmentation with cultured autologous fibroblasts.
Bovine collagen is an acceptable agent for vocal cord medialization; however, it produces only a temporary effect. As a foreign protein bovine collagen is susceptible to host collagenase and can induce immune response. Autologous collagen has become recently available, but it is less effective as a medialization agent. The study examines human skin fibroblasts growing in culture. Human skin bioptates were taken from the retroauricular area. Fibroblasts in culture were tested for scar contractility and ability to produce type I collagen (by flow cytometry with labeled antibodies). After five passages in culture the cells produced normal type I collagen, exhibited normal contractility, and did not induce no tumors in nude mice.
Berke
G
G
Laboratory of Head and Neck Surgery, University of California, Los Angeles School of Medicine, Los Angeles, California. gberke@mednet.usla.edu
Blumin
J
J
Sebastian
J
J
Keller
G
G
Revazova
E
E
D000818:Animals; D002417:Cattle; D017690:Cell Transplantation / Q000009:adverse effects / Q000379:methods; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D005347:Fibroblasts / Q000378:metabolism / Q000637:transplantation*; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D012449:Safety; D014182:Transplantation, Autologous; D014183:Transplantation, Heterologous; D014827:Vocal Cords / Q000601:surgery*
D003094:Collagen