Genome-wide expression-monitoring of jasmonate-responsive genes of Arabidopsis using cDNA arrays.
Jasmonates are generally considered to mediate signalling, such as defence responses, flowering and senescence. However, factors involved in the jasmonate signal-transduction pathway remain unclear. To clarify the functions and signalling mechanisms of jasmonates on a genome-wide level, we adopted a cDNA macroarray technique. We prepared nylon filters of a cDNA macroarray on which 2880 independent expressed sequence tag clones of Arabidopsis were blotted, and hybridized (33)P-labelled single-strand DNAs synthesized from mRNAs of methyl jasmonate (MeJA)-treated and untreated Arabidopsis plants to the nylon filters. By analysing the data from the cDNA macroarray, we identified many function-known and unknown genes as MeJA-responsive genes, and confirmed that the profiles of the expression showed good agreement with Northern-blot analysis. These results demonstrate the efficiency of the cDNA macroarray for systematically analysing jasmonate-responsive genes on a genome-wide scale.
Sasaki
Y
Y
Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama 226-8501, Japan.
Asamizu
E
E
Shibata
D
D
Nakamura
Y
Y
Kaneko
T
T
Awai
K
K
Masuda
T
T
Shimada
H
H
Takamiya
K
K
Tabata
S
S
Ohta
H
H
D000085:Acetates / Q000494:pharmacology*; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics*; D003517:Cyclopentanes / Q000494:pharmacology*; D020224:Expressed Sequence Tags; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000502:physiology*; D018745:Genome, Plant; D020411:Oligonucleotide Array Sequence Analysis; D054883:Oxylipins; D010937:Plant Growth Regulators / Q000494:pharmacology*; D015398:Signal Transduction
D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; C072239:methyl jasmonate