Antibody-induced activation of beta1 integrin receptors stimulates cAMP-dependent migration of breast cells on laminin-5.
The beta1 integrin-stimulating antibody TS2/16 induces cAMP-dependent migration of MCF-10A breast cells on the extracellular matrix protein laminin-5. TS2/16 stimulates a rise in intracellular cAMP within 20 min after plating. Pertussis toxin, which inhibits both antibody-induced migration and cAMP accumulation, targets the Galphai3 subunit of heterotrimeric G proteins in these cells, suggesting that Galphai3 may link integrin activation and migration via a cAMP signaling pathway.
Plopper
G E
GE
Department of Biological Sciences, University of Nevada, Las Vegas, Nevada 89154, USA. plopper@nye.nscee.edu
Huff
J L
JL
Rust
W L
WL
Schwartz
M A
MA
Quaranta
V
V
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000473:pathology*; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism / Q000494:pharmacology*; D017931:DNA Primers; D005260:Female; D020962:Heterotrimeric GTP-Binding Proteins / Q000378:metabolism*; D006801:Humans; D039201:Integrin alpha3beta1; D016023:Integrins / Q000378:metabolism*; D037342:Pertussis Toxin; D011233:Precipitin Tests; D017474:Receptors, Laminin / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D014407:Tumor Cells, Cultured; D010566:Virulence Factors, Bordetella / Q000494:pharmacology; D000097794:Kalinin
D000246:Adenosine Diphosphate Ribose; D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D000242:Cyclic AMP; D017931:DNA Primers; D020962:Heterotrimeric GTP-Binding Proteins; D039201:Integrin alpha3beta1; D016023:Integrins; D037342:Pertussis Toxin; D017474:Receptors, Laminin; D010566:Virulence Factors, Bordetella; D000097794:Kalinin