Pan1b (17betaHSD11)-enzymatic activity and distribution in the lung.
We describe a new member of the 17beta-hydroxysteroid dehydrogenase group of enzymes. Human Pan1b displays greatest activity with 5alpha-androstan-3alpha,17beta-diol (3alpha-Diol) as substrate, suggesting that it may be important in androgen metabolism. Enzymic activity was non-saturable with 3alpha-Diol but saturable with retinoids, although retinoids were not metabolized. Immunohistochemical studies on 10% formalin fixed and paraffin embedded sections of human tissues showed that Pan1b was present in acini and ciliated epithelia of the lung. In the fetus immuno reactivity was present in ciliated epithelia throughout gestation and staining appeared to be stronger in the second half of pregnancy. Pan1b was also expressed in the nonpigmented epithelium of the ciliary body, and in adrenocortical tumor cells. Although 3alpha-Diol is generally considered a degradation product of androgen metabolism it could have its own biological function. Pan1b may be an important modulator of the endocrine, or intracrine activity of this steroid.
Brereton
P
P
Molecular Hypertension Laboratory, Baker Medical Research Institute, PO Box 6492, 8008, Melbourne, Australia.
Suzuki
T
T
Sasano
H
H
Li
K
K
Duarte
C
C
Obeyesekere
V
V
Haeseleer
F
F
Palczewski
K
K
Smith
I
I
Komesaroff
P
P
Krozowski
Z
Z
D015067:17-Hydroxysteroid Dehydrogenases / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D000429:Alcohol Oxidoreductases; D000445:Aldehyde Oxidoreductases; D000595:Amino Acid Sequence; D000728:Androgens / Q000378:metabolism; D015113:Androstane-3,17-diol / Q000378:metabolism; D000735:Androstenedione / Q000378:metabolism; D000738:Androsterone / Q000378:metabolism; D002229:Carbenoxolone / Q000378:metabolism; D002460:Cell Line; D002923:Cilia / Q000201:enzymology; D004848:Epithelium / Q000201:enzymology; D004958:Estradiol / Q000378:metabolism; D004970:Estrone / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007231:Infant, Newborn; D008168:Lung / Q000196:embryology / Q000201:enzymology*; D008969:Molecular Sequence Data; D012176:Retinoids / Q000378:metabolism; D013379:Substrate Specificity; D013739:Testosterone / Q000378:metabolism; D014018:Tissue Distribution; D014132:Trachea / Q000201:enzymology; D014162:Transfection
D000728:Androgens; D012176:Retinoids; D015113:Androstane-3,17-diol; D004970:Estrone; D013739:Testosterone; D000735:Androstenedione; D004958:Estradiol; D000738:Androsterone; D015067:17-Hydroxysteroid Dehydrogenases; D000429:Alcohol Oxidoreductases; C118226:HSD17B11 protein, human; D000445:Aldehyde Oxidoreductases; D002229:Carbenoxolone