Inhibition of hBD-3, but not hBD-1 and hBD-2, mRNA expression by corticosteroids.
Intensive use of corticosteroids may be accompanied by increased susceptibility to infections; hence, we investigated the effects of dexamethasone on the expression of antimicrobial peptides, termed human beta-defensins (hBD), by cultured bronchial epithelial cells and mononuclear phagocytes. The results revealed that dexamethasone inhibited the (stimulated) expression of mRNA for hBD-3, but not hBD-1 and hBD-2 by these epithelial cells. Dexamethasone did not affect the (stimulated) mRNA expression of hBD-1 and hBD-2 by mononuclear phagocytes, whereas these cells did not express hBD-3 mRNA.
Duits
L A
LA
Department of Infectious Diseases, Leiden University Medical Center, Leiden, 2300 RC, The Netherlands.
Rademaker
M
M
Ravensbergen
B
B
van Sterkenburg
M A
MA
van Strijen
E
E
Hiemstra
P S
PS
Nibbering
P H
PH
D000305:Adrenal Cortex Hormones / Q000494:pharmacology*; D001980:Bronchi / Q000166:cytology; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D016209:Interleukin-8 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013296:Streptococcus pneumoniae / Q000502:physiology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D023083:beta-Defensins / Q000235:genetics*
D000305:Adrenal Cortex Hormones; C406407:DEFB1 protein, human; C106641:DEFB4A protein, human; D016209:Interleukin-8; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D023083:beta-Defensins; D003907:Dexamethasone; D007371:Interferon-gamma