Chromosomal localization, expression pattern, and promoter analysis of the mouse gene encoding adipocyte-specific secretory protein Acrp30.
Acrp30 is an abundantly expressed secretory protein exclusively synthesized in adipose tissue. Due to the dysregulation in various forms of obesity in humans and mice and its strong structural similarity to TNFalpha, it is currently under study as an important molecule involved in whole body energy homeostasis. Here we describe the sequence of mouse Acrp30 locus, define the intron/exon boundaries and map the gene to the telomere of mouse chromosome 16, syntenic to the human chromosomal locus 3q27. We demonstrate that alternative polyadenylation gives rise to two distinct mRNA species. We also show that Acrp30 expression is induced only at the late stages of mouse embryonic development. Finally, we have characterized the mouse Acrp30 promoter in tissue culture cells. We propose that Acrp30 promoter has the potential to drive strong adipocyte specific heterologous gene expression in transgenic mice.
Das
K
K
Department of Cell Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York, 10461, USA.
Lin
Y
Y
Widen
E
E
Zhang
Y
Y
Scherer
P E
PE
D016475:3T3 Cells; D017667:Adipocytes / Q000378:metabolism; D052242:Adiponectin; D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D001798:Blood Proteins / Q000096:biosynthesis* / Q000235:genetics*; D015152:Blotting, Northern; D015139:Blotting, Southern; D019556:COS Cells; D002478:Cells, Cultured; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3; D017124:Conserved Sequence; D005091:Exons; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D036341:Intercellular Signaling Peptides and Proteins; D007438:Introns; D008156:Luciferases / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D008957:Models, Genetic; D016366:Open Reading Frames; D010957:Plasmids / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011506:Proteins; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D021101:Radiation Hybrid Mapping; D017422:Sequence Analysis, DNA; D013997:Time Factors; D014018:Tissue Distribution; D014162:Transfection
D052242:Adiponectin; D001798:Blood Proteins; D036341:Intercellular Signaling Peptides and Proteins; D011506:Proteins; D012333:RNA, Messenger; D012313:RNA; D008156:Luciferases