Identification of two rodent genes encoding homologues to seminal vesicle autoantigen: a gene family including the gene for prolactin-inducible protein.
We cloned two new paralogous genes that encode proteins homologous to seminal vesicle autoantigen (SVA) in rodents. The open reading frame of one mouse gene encodes a polypeptide consisting of 151 amino acid residues which has 43% identity to SVA. RT-PCR analysis showed selective expression in the colon, and thus the protein was tentatively named "SVA-like protein in the colon (SLP-C)". The other mouse gene has an open reading frame encoding 144 amino acid residues with 46 and 65% identity to SVA and SLP-C, respectively. Expression of this gene was detected in the mammary, submaxillary, parotid, and lacrimal glands, and this protein was named "SLP in the mammary gland (SLP-M)". Orthologs of both genes were also found in rats. The three homologous genes coding for SVA, SLP-C, and SLP-M may have been generated by gene duplication with divergence of tissue expression in the course of evolution. They comprise a unique structurally-related gene family. Moreover, these genes share significant sequence homology with that of another secretory glycoprotein, prolactin-inducible protein.
Yoshida
M
M
Department of Obstetrics and Gynecology, Yamagata University School of Medicine, Yamagata, 990-9585, Japan.
Kaneko
M
M
Kurachi
H
H
Osawa
M
M
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D017209:Apoptosis; D016282:Aspartic Acid Endopeptidases; D001324:Autoantigens / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D003106:Colon / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D005260:Female; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics*; D007765:Lacrimal Apparatus / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000378:metabolism; D051379:Mice; D005810:Multigene Family; D016366:Open Reading Frames; D010306:Parotid Gland / Q000378:metabolism; D010455:Peptides / Q000737:chemistry; D010802:Phylogeny; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029607:Seminal Vesicle Secretory Proteins; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013363:Submandibular Gland / Q000378:metabolism; D014018:Tissue Distribution
D000596:Amino Acids; D001324:Autoantigens; D002352:Carrier Proteins; D018076:DNA, Complementary; D006023:Glycoproteins; D010455:Peptides; D029607:Seminal Vesicle Secretory Proteins; C085073:seminal vesicle autoantigen; D016282:Aspartic Acid Endopeptidases; C423002:Sval1 protein, mouse; C486034:Sval1 protein, rat; C423003:Sval2 protein, mouse; C486035:Sval2 protein, rat