Agmatine uptake by cultured hamster kidney cells.
Agmatine, the product of arginine decarboxylation, has been recently found in a wide variety of animal tissues. In spite of the emergent interest on agmatine in animals, the mechanism of agmatine uptake in mammalian cells has been scarcely studied. An analysis of radiolabeled agmatine uptake was carried out by using a classical, kinetic approach with BHK-21 hamster kidney cells in culture. A high affinity, temperature- and energy-dependent agmatine transport system in BHK-21 kidney cells is here kinetically characterized which seems to be a "general" transporter shared by di- and triamines and different to a highly specific carrier for the tetraamine spermine.
del Valle
A E
AE
Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, E-29071 Málaga, Spain.
Paz
J C
JC
Sánchez-Jiménez
F
F
Medina
M A
MA
D000376:Agmatine / Q000493:pharmacokinetics*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D002794:Choline / Q000494:pharmacology; D006224:Cricetinae; D007668:Kidney; D007700:Kinetics; D008353:Mannitol / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D013096:Spermine / Q000378:metabolism; D013379:Substrate Specificity; D014316:Tritium
D002352:Carrier Proteins; D014316:Tritium; D013096:Spermine; D008353:Mannitol; D000376:Agmatine; D002794:Choline; D011188:Potassium