Analysis of trimethylsilyl derivatization products of phosphatidylethanol by gas chromatography-mass spectrometry.
For the detection of rare phospholipid, phosphatidylethanol (PEt), GC-MS analysis method was adopted for the detection of derivatization products of PEt by N,O-bis (trimethylsilyl) trifluroacetamide (BSTFA). A re-structured molecule derived from PEt, ethyl bis (trimethylsilyl)-phosphate was found from search of Wiley database. This molecule can be used as a marker for PEt analysis. The molecular formula was C8H23O4PSi2 and weight of the formula was 270.09.
Yon
C
C
Institute of Biomedical Science and Department of Biochemistry, College of Medicine, Hanyang University, Seoul, Korea.
Han
J S
JS
D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D020404:Glycerophospholipids / Q000737:chemistry*; D014297:Trimethylsilyl Compounds / Q000737:chemistry*
D020404:Glycerophospholipids; D014297:Trimethylsilyl Compounds; C051521:phosphatidylethanol; C047270:N,N-bis(trimethylsilyl)-2,2,2-trifluoroacetamide