[Cloning and restriction analysis of saw1--a key gene for differentiation of Streptomyces ansochromogenes].
saw1--a essential gene for sporulation of Streptomyces ansochromogenes was cloned, 7 kb of DNA fragment in Streptomyces plasmid pIJ702 could recover the sporulation of saw1-deficient strain W51 which could not form spore with white phenotype of aerial hyphae. The recombinant plasmid was deleted by some restriction enzymes, the result showed that 2.5 kb DNA fragment in pIJ702 still has function to recover sporulation of W51. The sequencing and expression of saw1 gene is carrying out at present.
Tan
H
H
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080.
Wang
L
L
Tian
Y
Y
Yang
H
H
D003001:Cloning, Molecular; D053938:DNA Fragmentation; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D013302:Streptomyces / Q000235:genetics*
D004269:DNA, Bacterial