title: The balance between protective immunity and pathogenesis in tropical theileriosis: what we need to know to design effective vaccines for the future.
abstract: The tick-borne protozoan parasite, Theileria annulata, causes an overwhelming disease in Friesian cattle, imported to improve productivity, in a large area of the world. The parasite invades bovine macrophages and induces aberrant changes which seem pivotal in triggering disease in naïve susceptible animals: parasite infected cells acquire dendritic cell features and over-activate CD4+ and CD8+ T cells. Elevated levels of interferon-gamma (IFN-gamma) are induced and B cells are developmentally arrested in the light zone of germinal centres. Infected macrophages are refractory to the effects of IFN-gamma and indeed flourish in its presence. High levels of pro-inflammatory cytokines, as evinced by high acute phase protein responses, probably also play a role in pathology. However, animals can become immune to further challenge. Cellular immune responses involving macrophages, natural killer cells and CD8+ T cells play a major role in recovery and subsequent maintenance of immunity. The main target for immunity appears to be the parasite infected macrophage, as attenuated cell lines can protect and are used as vaccines. Cloned lines selected for low cytokine production protect with no associated pathological reactions. Theileria annulata causes a relatively mild disease in an indigenous breed of cattle, which is associated with lower acute phase protein responses (controlled by macrophage cytokines). Thus the initial host-parasite interactions must determine the balance between immunity and pathogenesis. New generation vaccines to T. annulata should both induce active immunity and suppress pathology.
authors.affiliation: Roslin Institute, Roslin, Midlothian, EH25 9PS, Scotland, UK.
authors.forename: E J
authors.initials: EJ
authors.lastname: Glass
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002417:Cattle; D007371:Interferon-gamma / Q000096:biosynthesis; D007694:Killer Cells, Natural / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D016052:Protozoan Vaccines / Q000138:chemical synthesis* / Q000276:immunology; D016796:Theileria annulata / Q000472:pathogenicity; D013801:Theileriasis / Q000276:immunology / Q000517:prevention & control*
chemical_list: D016052:Protozoan Vaccines; D007371:Interferon-gamma