title: Microbiological and biochemical study of coffee fermentation.
abstract: The coffee fermentation microflora were rich and mainly constituted of aerobic Gram-negative bacilli, with Erwinia and Klebsiella genuses at the highest frequencies. The best population increase was observed with lactic acid bacteria and yeasts, whereas those microorganisms that counted on a pectin medium remained constant during the fermentation step. Qualitatively, lactic acid bacteria belonged mainly to Leuconostoc mesenteroides species but the others microflora were relatively heterogeneous. The microorganisms isolated on pectin medium were Enterobacteriaceae, identified as Erwinia herbicola and Klebsiella pneumoniae, not reported as strong pectolytic strains. Throughout coffee fermentation, 60% of the simple sugars were degraded by the total microflora and not specifically by pectolytic microorganisms.
authors.affiliation: USTL F-34095, GBSA-MBI CC 23 Université de Montpellier II, Place Eugène Bataillon, 34095 Montpellier Cedex 5, France.
authors.forename: S
authors.initials: S
authors.lastname: Avallone
authors.forename: B
authors.initials: B
authors.lastname: Guyot
authors.forename: J M
authors.initials: JM
authors.lastname: Brillouet
authors.forename: E
authors.initials: E
authors.lastname: Olguin
authors.forename: J P
authors.initials: JP
authors.lastname: Guiraud
mesh_terms: D002241:Carbohydrates / Q000032:analysis; D002849:Chromatography, Gas; D002852:Chromatography, Ion Exchange; D003069:Coffee / Q000737:chemistry / Q000382:microbiology*; D015169:Colony Count, Microbial; D004885:Erwinia / Q000302:isolation & purification; D000431:Ethanol / Q000032:analysis; D005285:Fermentation; D007709:Klebsiella / Q000302:isolation & purification; D007934:Leuconostoc / Q000302:isolation & purification; D015003:Yeasts / Q000302:isolation & purification
chemical_list: D002241:Carbohydrates; D003069:Coffee; D000431:Ethanol