title: [Effect of T-lymphocytes on hematopoietic progenitors in patients with paroxysmal nocturnal hemoglobinuria].
abstract: OBJECTIVE
  To study the effect of T-lymphocytes on hematopoietic progenitors in patients with paroxysmal nocturnal hemoglobinuria (PNH).
  
  
  METHODS
  Phytohemagglutinin-stimulating T-lymphocytes conditioned medium(PHA-TCM) prepared from PNH(PHA-TCM-PNH) and normal blood(PHA-TCM-N) was added to the cultures of PNH or normal CFU-GM and BFU-E.
  
  
  RESULTS
  In cultures containing PHA-TCM-PNH prepared from newly diagnosed PNH patients, the yields of CFU-GM and BFU-E from either PNH or normal bone marrow were reduced more than they did in cultures containing PHA-TCM-N; but in cultures containing PHA-TCM-PNH prepared from PNH patients in remission, they were normal.
  
  
  CONCLUSIONS
  T-lymphocytes from newly diagnosed PNH patients exerts an inhibitory effect on the in vitro growth of CFU-GM and BFU-E.
authors.affiliation: Department of Hematology, First Affiliated Hospital, Hubei Medical University, Wuhan 430060.
authors.forename: Q
authors.initials: Q
authors.lastname: Gao
authors.forename: H
authors.initials: H
authors.lastname: Xia
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000293:Adolescent; D000328:Adult; D018957:CD59 Antigens / Q000378:metabolism; D002478:Cells, Cultured; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D006457:Hemoglobinuria, Paroxysmal / Q000473:pathology*; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D018957:CD59 Antigens; D007376:Interleukin-2; D016178:Granulocyte-Macrophage Colony-Stimulating Factor