Use of mass spectrometry-derived data to annotate nucleotide and protein sequence databases.
Mass spectrometry-based proteomic methodologies can be used to annotate both nucleotide and protein sequence databases. Because such data have to be derived from proteins, they can be used to identify coding regions of the genome as well as provide the complete primary sequence of proteins and their expression patterns and post-translational modifications.
Mann
M
M
Protein Interaction Laboratory (PIL), Center for Experimental Bioinformatics, University of Southern Denmark, Campusvej 55, DK-5230, and MDS-Protana, Staermosegaardsvej 6, DK-5230, Odense M, Denmark. mann@bmb.sdu.dk
Pandey
A
A
D000818:Animals; D018387:Codon, Initiator; D016208:Databases, Factual; D005091:Exons; D020224:Expressed Sequence Tags; D005786:Gene Expression Regulation; D023281:Genomics / Q000379:methods*; D016045:Human Genome Project; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D016366:Open Reading Frames; D011499:Protein Processing, Post-Translational; D021382:Protein Sorting Signals / Q000235:genetics; D011506:Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA / Q000379:methods; D020539:Sequence Analysis, Protein / Q000379:methods
D018387:Codon, Initiator; D021382:Protein Sorting Signals; D011506:Proteins