title: [Culture of human corneal epithelial cells].
abstract: OBJECTIVE
  To select culture technique of human epithelial cells for grafts.
  
  
  METHODS
  Epithelial cells of limbus were collected by improved enzymatic disaggregation or explant technique growth curves and to calculate doubling time. DNA synthesis of cells were measured with 3H-thymidine incorporation and liquid scintillation techniques.
  
  
  RESULTS
  Doubling time of cells which were collected by enzymatic disaggregation and by explant technique were 54.15 +/- 4.28 h and 67.88 +/- 1.96 h (P < 0.01). Cellular DNA synthesis of the former was more active (P < 0.01).
  
  
  CONCLUSIONS
  Epithelial cells which were collected by improved enzymatic disaggregation from limbus include stem cells of corneal epithelium. These cells show more active cellulde proliferation and are fitter for use as grafts.
authors.affiliation: Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China.
authors.forename: X
authors.initials: X
authors.lastname: Zhong
authors.forename: X
authors.initials: X
authors.lastname: Gong
authors.forename: Z
authors.initials: Z
authors.lastname: Dai
authors.forename: H
authors.initials: H
authors.lastname: Zheng
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods; D004247:DNA / Q000096:biosynthesis; D019573:Epithelium, Corneal / Q000166:cytology*; D006801:Humans; D016850:Limbus Corneae / Q000166:cytology
chemical_list: D004247:DNA