Maltose-binding protein as a fusion tag for the localization and purification of cloned proteins in Dictyostelium.
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Adolf-Butenandt-Institut/Zellbiologie, Schillerstrasse 42, D-80336 Münich, Germany. Ralph.Graef@lrz.uni-muenchen.de
D000818:Animals; D001671:Biochemistry / Q000379:methods*; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism*; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D004023:Dictyostelium / Q000737:chemistry / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D019084:Fluorescent Antibody Technique, Indirect; D005821:Genetic Techniques; D005822:Genetic Vectors; D058619:Maltose-Binding Proteins; D010957:Plasmids; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000378:metabolism
D002352:Carrier Proteins; D058619:Maltose-Binding Proteins; D011993:Recombinant Fusion Proteins; D017346:Protein Serine-Threonine Kinases