2D or not 2D. Two-dimensional gel electrophoresis.
2D gel electrophoresis is the technology that everyone loves to hate-it requires manual dexterity and precision to reproduce precisely and is thus not well-suited as a high-throughput technology. Although almost everyone would like to replace it, the resolution and sensitivity it offers are exquisite and unsurpassed if one wants a global view of cellular activity. There have been several recent developments, for example, the detection of low abundance proteins, and the resolution possible with narrow-range IPG gels.
Fey
S J
SJ
Centre for Proteome Analysis, University of Southern Denmark, International Science Park Odense, Forskerparken 10B, DK-5230, Odense M, Denmark. sjf@cpa.spo.dk
Larsen
P M
PM
D000818:Animals; D019295:Computational Biology; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods* / Q000592:standards* / Q000639:trends; D006801:Humans; D020543:Proteome / Q000032:analysis; D012680:Sensitivity and Specificity
D020543:Proteome