Solution structure of human immunodeficiency virus type 1 Vpr(13-33) peptide in micelles.
Human immunodeficiency virus type 1 protein R (HIV-1 Vpr) promotes nuclear entry of viral nucleic acids in nondividing cells, causes G2 cell cycle arrest and is involved in cellular differentiation and cell death. Also, Vpr subcellular localization is as variable as its functions. It is known that, consistent with its role in nuclear transport, Vpr localizes to the nuclear envelope of human cells. Further, a reported ion channel activity of Vpr obviously is dependent on its localization in or at membranes. We focused our structural studies on the secondary structure of a peptide consisting of residues 13-33 of HIV-1 Vpr in micelles. Employing nuclear magnetic resonance and circular dichroism spectroscopy we found this part of Vpr, known to be essential for nuclear localization, to be almost completely alpha helical. Our results provide structural data suggesting residues 13-33 of Vpr to form an amphipathic, leucine-zipper-like alpha helix that serves as a basis for interactions with a variety of viral and cellular factors.
Engler
A
A
Lehrstuhl für Biopolymere, Universität Bayreuth, Germany.
Stangler
T
T
Willbold
D
D
D000595:Amino Acid Sequence; D002942:Circular Dichroism; D016336:Gene Products, vpr / Q000737:chemistry*; D015497:HIV-1 / Q000737:chemistry*; D008823:Micelles; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D010446:Peptide Fragments / Q000737:chemistry*; D010767:Phosphorylcholine / Q000031:analogs & derivatives*; D017433:Protein Structure, Secondary; D012996:Solutions; D054325:vpr Gene Products, Human Immunodeficiency Virus
D016336:Gene Products, vpr; D008823:Micelles; D010446:Peptide Fragments; D012996:Solutions; D054325:vpr Gene Products, Human Immunodeficiency Virus; C422457:vpr peptide (13-33), Human immunodeficiency virus 1; D010767:Phosphorylcholine; C028810:dodecylphosphocholine