title: Enhancement of seed oil content by expression of glycerol-3-phosphate acyltransferase genes.
abstract: Arabidopsis thaliana was transformed with a plastidial safflower glycerol-3-phosphate acyltransferase (GPAT) and an Escherichia coli GPAT. The genes were used directly and in modified forms with, as applicable, the plastidial targeting sequence removed, and with an endoplasmic reticulum targeting sequence added. Seeds of plants transformed using only the vector were indistinguishable in oil content from wild-type control plants. All other gene constructs increased seed oil content. The unmodified safflower gene (spgpat) produced oil increases ranging from 10 to 21%. On average, the greatest increase (+22%) was observed in seeds of transformants carrying the spgpat with the targeting peptide removed. The E. coli plsB gene increased seed oil content by an average of 15%.
authors.affiliation: National Research Council of Canada, Plant Biotechnology Institute, 110 Gymnasium Place, Saskatoon, SK, Canada S7N 0W9.
authors.forename: R K
authors.initials: RK
authors.lastname: Jain
authors.forename: M
authors.initials: M
authors.lastname: Coffey
authors.forename: K
authors.initials: K
authors.lastname: Lai
authors.forename: A
authors.initials: A
authors.lastname: Kumar
authors.forename: S L
authors.initials: SL
authors.lastname: MacKenzie
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D019659:Asteraceae / Q000201:enzymology* / Q000235:genetics; D001709:Biotechnology / Q000379:methods; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods; D005992:Glycerol-3-Phosphate O-Acyltransferase / Q000235:genetics* / Q000378:metabolism*; D010938:Plant Oils / Q000737:chemistry; D030821:Plants, Genetically Modified / Q000201:enzymology; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012639:Seeds
chemical_list: D010938:Plant Oils; D011993:Recombinant Fusion Proteins; D005992:Glycerol-3-Phosphate O-Acyltransferase