title: Hydroxypropyl-distarch phosphate from Tapioca starch reduces zinc and iron absorption, but not calcium and magnesium absorption, in rats.
abstract: Male rats were fed a fiber-free, purified diet containing either gelatinized tapioca starch that was not modified chemically (TS, 50 g/kg diet) or gelatinized chemically modified tapioca starch (CMS, 50 g/kg) for 21 d. TS was used as the control. The six kinds of gelatinized hydroxypropyl distarch phosphate (HDP) from tapioca with two different degrees of substitution (DS) and three different degrees of cross-linking (DC) were used as CMS sources. The wet weight and moisture of fecal output of the rats fed HDP with higher DS were 100 and 20% greater than that in the control rats, respectively. The weights of cecal wall and cecal contents were also 30 and 50% higher in the rats fed HDP with higher DS than those in the control rats. The pH of the cecal contents was more acidic in the rats fed HDP with higher DS than that in the control rats. Fecal excretion of bile acids was 40% higher in the rats fed HDP with higher DS than in the control rats. These effects of HDP were only slightly affected by the DC. The plasma cholesterol concentration was 16% lower in the rats fed HDP with higher DS and highest DC than in the control rats. The concentrations of liver lipids and plasma triglycerides and the cecal pool of organic acids were not affected by diet. The apparent absorptions of Ca and Mg were not affected by diet, but those of Zn and Fe were 75 and 70% lower in the rats fed HDP with higher DS than in the control rats. These results suggest that the physiological effects of HDP depend on the DS but not on the DC.
authors.affiliation: Department of Biological Resources, Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama 790, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kishida
authors.forename: Y
authors.initials: Y
authors.lastname: Nakai
authors.forename: K
authors.initials: K
authors.lastname: Ebihara
mesh_terms: D000818:Animals; D002118:Calcium / Q000493:pharmacokinetics; D002432:Cecum / Q000378:metabolism; D005243:Feces / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D006895:Hydroxyethyl Starch Derivatives / Q000031:analogs & derivatives / Q000494:pharmacology*; D007408:Intestinal Absorption / Q000187:drug effects*; D007501:Iron / Q000493:pharmacokinetics*; D008274:Magnesium / Q000493:pharmacokinetics; D008297:Male; D002365:Manihot / Q000737:chemistry; D009929:Organ Size; D051381:Rats; D017208:Rats, Wistar; D015032:Zinc / Q000493:pharmacokinetics*
chemical_list: D006895:Hydroxyethyl Starch Derivatives; C050767:hydroxypropyl distarch phosphate; D007501:Iron; D008274:Magnesium; D015032:Zinc; D002118:Calcium