title: Genetic analysis of full-length genomes and subgenomic sequences of TT virus-like mini virus human isolates.
abstract: The phylogenetic relationship between the complete genomic sequences of ten Japanese and one French isolate of TT virus-like mini virus (TLMV) was investigated. Analysis of the variability of the nucleotide sequences and the detection of signature patterns for overlapping genes suggested that ORFs 1 and 2 are probably functional. However, this was not the case for a putative third ORF, ORF3. Throughout the viral genome, several nucleotide or amino acid motifs that are conserved in circoviruses such as TT virus (TTV) and chicken anaemia virus were identified. Phylogenetic analysis distinguished three main groups of TLMV and allowed the identification of putative recombination breakpoints in the untranslated region. TLMV genomes were detected by PCR in the plasma of 38/50 French blood donors tested and were also identified in peripheral blood mononuclear cells, faeces and saliva. A phylogenetic study of 37 TLMV strains originating from France, Japan and Brazil showed that groupings were not related to geographical origin.
authors.affiliation: Unité des Virus Emergents, EA 871, Laboratoire de Virologie Moléculaire, Établissement Français du Sang 'Alpes-Méditerranée', 149 Boulevard Baille, 13005 Marseille, France1.
authors.forename: Philippe
authors.initials: P
authors.lastname: Biagini
authors.affiliation: Unité des Virus Emergents, EA 871, Laboratoire de Virologie Moléculaire, Établissement Français du Sang 'Alpes-Méditerranée', 149 Boulevard Baille, 13005 Marseille, France1.
authors.forename: Pierre
authors.initials: P
authors.lastname: Gallian
authors.affiliation: Unité des Virus Emergents, EA 871, Laboratoire de Virologie Moléculaire, Établissement Français du Sang 'Alpes-Méditerranée', 149 Boulevard Baille, 13005 Marseille, France1.
authors.forename: Houssam
authors.initials: H
authors.lastname: Attoui
authors.affiliation: Unité des Virus Emergents, EA 871, Laboratoire de Virologie Moléculaire, Établissement Français du Sang 'Alpes-Méditerranée', 149 Boulevard Baille, 13005 Marseille, France1.
authors.forename: Mhammed
authors.initials: M
authors.lastname: Touinssi
authors.affiliation: Unité des Virus Emergents, EA 871, Laboratoire de Virologie Moléculaire, Établissement Français du Sang 'Alpes-Méditerranée', 149 Boulevard Baille, 13005 Marseille, France1.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Cantaloube
authors.affiliation: Unité des Virus Emergents, EA 871, Laboratoire de Virologie Moléculaire, Tropicale et Transfusionnelle, Faculté de Médecine, 27 Boulevard Jean Moulin, 13005 Marseille, France2.|Unité des Virus Emergents, EA 871, Laboratoire de Virologie Moléculaire, Établissement Français du Sang 'Alpes-Méditerranée', 149 Boulevard Baille, 13005 Marseille, France1.
authors.forename: Philippe
authors.initials: P
authors.lastname: de Micco
authors.affiliation: Unité des Virus Emergents, EA 871, Laboratoire de Virologie Moléculaire, Tropicale et Transfusionnelle, Faculté de Médecine, 27 Boulevard Jean Moulin, 13005 Marseille, France2.
authors.forename: Xavier
authors.initials: X
authors.lastname: de Lamballerie
mesh_terms: D001482:Base Composition; D001938:Brazil / Q000453:epidemiology; D018173:Circoviridae Infections / Q000453:epidemiology / Q000821:virology; D017925:Circovirus / Q000737:chemistry / Q000235:genetics*; D017124:Conserved Sequence / Q000235:genetics; D004266:DNA Virus Infections / Q000453:epidemiology / Q000821:virology*; D004279:DNA, Viral / Q000097:blood / Q000235:genetics; D005243:Feces / Q000821:virology; D005602:France / Q000453:epidemiology; D016679:Genome, Viral; D006801:Humans; D007564:Japan / Q000453:epidemiology; D007963:Leukocytes, Mononuclear / Q000821:virology; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D011995:Recombination, Genetic / Q000235:genetics; D012463:Saliva / Q000821:virology; D022783:Torque teno virus / Q000737:chemistry / Q000235:genetics*
chemical_list: D004279:DNA, Viral