Marine corrinoid-binding proteins for the direct determination of vitamin B12 by radioassay.
Under conditions of vitamin B12 deficiency the marine phytoplankton Thalassiosira pseudonana secretes into the growth medium a protein (Gm protein) that binds B12 specifically with an affinity constant of 2 x 10(11) M(-1). When Gm protein was used as a B12-specific binder in radioassays for the direct determination of the vitamin, the detection limit of the technique approached 5 pg B12/mL. In its natural state, Gm protein is oligomeric having molecular weight of over 400 kDa. Modification of the solution environment of this protein produces unique changes in its tertiary and quaternary structure. The amino acid and submit composition of the protein is reported.
Sahni
M K
MK
Departments of Chemistry and Biology, William Paterson University, Wayne, New Jersey 07470, USA.
Spanos
S
S
Wahrman
M Z
MZ
Sharma
G M
GM
D001667:Binding, Competitive; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D045728:Corrinoids; D004591:Electrophoresis, Polyacrylamide Gel; D010839:Phytoplankton / Q000737:chemistry*; D011166:Porphyrins / Q000378:metabolism*; D012680:Sensitivity and Specificity; D014805:Vitamin B 12 / Q000032:analysis*
D002352:Carrier Proteins; D045728:Corrinoids; D011166:Porphyrins; D014805:Vitamin B 12