title: Molecular cloning of a novel ubiquitin-like protein, UBIN, that binds to ER targeting signal sequences.
abstract: To identify proteins that interact with HSP47, an endoplasmic reticulum (ER)-resident molecular chaperone, a yeast two-hybrid screening was performed using mouse full-length HSP47 including an N-terminal signal sequence as a bait. Analysis of several positive clones led to the identification and cloning of a novel gene, ubin, encoding a ubiquitin-like protein. Unlike other ubiquitin-like proteins, UBIN was shown to interact with signal sequences of various secretory and ER-luminal proteins, including HSP47, but not interact with signal sequences of mitochondrial targeting in two-hybrid system. The possible function of UBIN will be discussed with regards to novel characteristics of binding to signal sequences for ER targeting.
authors.affiliation: Department of Molecular and Cellular Biology, Kyoto University, Sakyo-ku, Kyoto, 606-8397, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Matsuda
authors.forename: T
authors.initials: T
authors.lastname: Koide
authors.forename: T
authors.initials: T
authors.lastname: Yorihuzi
authors.forename: N
authors.initials: N
authors.lastname: Hosokawa
authors.forename: K
authors.initials: K
authors.lastname: Nagata
mesh_terms: D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D000071183:Autophagy-Related Proteins; D001665:Binding Sites; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D020869:Gene Expression Profiling; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D011485:Protein Binding; D021382:Protein Sorting Signals / Q000235:genetics / Q000502:physiology*; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017384:Sequence Deletion / Q000235:genetics; D014407:Tumor Cells, Cultured; D020798:Two-Hybrid System Techniques; D014452:Ubiquitins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
chemical_list: D000071183:Autophagy-Related Proteins; D002352:Carrier Proteins; D009687:Nuclear Proteins; D021382:Protein Sorting Signals; D012333:RNA, Messenger; C486020:UBIN protein, mouse; C421907:UBQLN4 protein, human; D014452:Ubiquitins