title: Genome-wide expression-monitoring of jasmonate-responsive genes of Arabidopsis using cDNA arrays.
abstract: Jasmonates are generally considered to mediate signalling, such as defence responses, flowering and senescence. However, factors involved in the jasmonate signal-transduction pathway remain unclear. To clarify the functions and signalling mechanisms of jasmonates on a genome-wide level, we adopted a cDNA macroarray technique. We prepared nylon filters of a cDNA macroarray on which 2880 independent expressed sequence tag clones of Arabidopsis were blotted, and hybridized (33)P-labelled single-strand DNAs synthesized from mRNAs of methyl jasmonate (MeJA)-treated and untreated Arabidopsis plants to the nylon filters. By analysing the data from the cDNA macroarray, we identified many function-known and unknown genes as MeJA-responsive genes, and confirmed that the profiles of the expression showed good agreement with Northern-blot analysis. These results demonstrate the efficiency of the cDNA macroarray for systematically analysing jasmonate-responsive genes on a genome-wide scale.
authors.affiliation: Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama 226-8501, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Sasaki
authors.forename: E
authors.initials: E
authors.lastname: Asamizu
authors.forename: D
authors.initials: D
authors.lastname: Shibata
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: T
authors.initials: T
authors.lastname: Kaneko
authors.forename: K
authors.initials: K
authors.lastname: Awai
authors.forename: T
authors.initials: T
authors.lastname: Masuda
authors.forename: H
authors.initials: H
authors.lastname: Shimada
authors.forename: K
authors.initials: K
authors.lastname: Takamiya
authors.forename: S
authors.initials: S
authors.lastname: Tabata
authors.forename: H
authors.initials: H
authors.lastname: Ohta
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics*; D003517:Cyclopentanes / Q000494:pharmacology*; D020224:Expressed Sequence Tags; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000502:physiology*; D018745:Genome, Plant; D020411:Oligonucleotide Array Sequence Analysis; D054883:Oxylipins; D010937:Plant Growth Regulators / Q000494:pharmacology*; D015398:Signal Transduction
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; C072239:methyl jasmonate