New cationic liposomes for gene transfer into mammalian cells with high efficiency and low toxicity.
Novel cationic amphiphiles, based on hydrophobic cholesterol linked to L-lysinamide or L-ornithinamide, were designed and tested as nonviral gene transfer vectors. Each amide form of amino acid was conjugated to cholesterol by a carbamate ester bond to facilitate efficient degradation in animal cells. Cytotoxicity tests were performed for some cell lines. The transfection efficiency of the amphiphiles on different cell lines was evaluated as a liposomal solution in the presence of the fusogenic helper lipid, dioleoyl phosphatidylethanolamine (DOPE). The efficiency was also compared with other generally used gene carriers, such as lipofectin, 3 beta[N-(N'N'-dimethylaminoethane)-carbamoyl] cholesterol (DC-Chol) liposome, and polyethylenimine (PEI).
Choi
J S
JS
School of Chemistry & Molecular Engineering, Seoul National University, San 56-1, Shillim-dong, Kwanak-ku, Seoul, 151-742, Korea.
Lee
E J
EJ
Jang
H S
HS
Park
J S
JS
D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002784:Cholesterol / Q000031:analogs & derivatives / Q000737:chemistry* / Q000633:toxicity; D004587:Electrophoresis, Agar Gel; D018014:Gene Transfer Techniques; D006801:Humans; D007202:Indicators and Reagents; D008081:Liposomes / Q000737:chemistry* / Q000633:toxicity; D018625:Microscopy, Atomic Force; D010957:Plasmids; D014162:Transfection; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D007202:Indicators and Reagents; C424784:K-Chol cpd; D008081:Liposomes; C424785:O-Chol cpd; D002784:Cholesterol; D001616:beta-Galactosidase