Molecular architecture of the amplified nucleoli of Xenopus oocytes.
An understanding of the functional organization of nucleoli, the sites of ribosome biosynthesis, is limited by the present uncertainty about the topological arrangement of the transcribing rRNA genes. Since studies with "standard" nucleoli from somatic cells produced conflicting results, we have examined the amplified nucleoli of Xenopus oocytes. These nucleoli are unique in that they contain high copy numbers of rRNA genes, are not attached to chromosomes, lack non-ribosomal DNA and can be examined in light microscopic spread preparations of nuclear contents. By immunostaining and confocal microscopy we show that in growing stage IV oocytes the sites of rDNA are surrounded by the dense fibrillar component. The rDNA is actively transcribed as revealed by BrUTP injection into oocytes and localization of components of the nucleolar transcription machinery (RNA polymerase I and the transcription factor UBF). At the ultrastructural level, the rDNA sites correlate with the fibrillar centers of amplified nucleoli fixed in situ. The results provide clear evidence that the transcriptionally active rRNA genes are confined to the fibrillar centers of the oocyte nucleoli and open the possibility to analyze the protein composition of almost native, transcriptionally highly active nucleolar chromatin by immunofluorescence microscopy.
Mais
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C
Department of Cell and Developmental Biology, Biocenter of the University of Würzburg, Am Hubland, 97074 Würzburg, Germany.
Scheer
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U
D000818:Animals; D002466:Cell Nucleolus / Q000648:ultrastructure*; D005260:Female; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D009865:Oocytes / Q000648:ultrastructure*; D012335:RNA, Ribosomal / Q000235:genetics / Q000648:ultrastructure; D014982:Xenopus laevis
D012335:RNA, Ribosomal