Histamine prevents polyamine accumulation in mouse C57.1 mast cell cultures.
The effects of histamine on polyamine uptake and metabolism was studied in a mouse mast cell line (C57.1), as a cell model in which both biogenic amines are important for maintaining cell function and viability. Results obtained after incubations with exogenous histamine indicated that histamine prevents polyamine accumulation by affecting polyamine uptake. A plasma membrane transport system for polyamines has been also studied in mast cells. It seems to be a Na(+)-dependent uptake with high affinity for both spermine and spermidine and lower affinity for putrescine and agmatine. Polyamine uptake was reduced in both cells treated with exogenous histamine and histamine-preloaded cells. However, ornithine decarboxylase activity and cell proliferation were not affected by histamine. Incubation with histamine enhanced the spermidine/spermine acetyl transferase induction caused by N(1)-ethyl-N(11)-[(cyclopropyl)methyl]-4,8-diazaundecane, suggesting that polyamine acetylation could be another mechanism by which histamine prevents polyamine accumulation in C57.1 mast cells.
Fajardo
I
I
Department of Molecular Biology and Biochemistry, Faculty of Sciences, University of Málaga, Campus of Teatinos, Spain.
Urdiales
J L
JL
Paz
J C
JC
Chavarría
T
T
Sánchez-Jiménez
F
F
Medina
M A
MA
D000123:Acetyltransferases / Q000378:metabolism; D000376:Agmatine / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport; D001854:Bone Marrow Cells / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006632:Histamine / Q000494:pharmacology*; D007700:Kinetics; D008407:Mast Cells / Q000737:chemistry*; D051379:Mice; D008810:Mice, Inbred C57BL; D009955:Ornithine Decarboxylase / Q000378:metabolism; D011073:Polyamines / Q000378:metabolism* / Q000493:pharmacokinetics; D011700:Putrescine / Q000378:metabolism / Q000494:pharmacology; D012701:Serotonin / Q000494:pharmacology; D013095:Spermidine / Q000378:metabolism / Q000494:pharmacology; D013096:Spermine / Q000378:metabolism / Q000494:pharmacology; D013997:Time Factors
D011073:Polyamines; D013096:Spermine; D012701:Serotonin; D000376:Agmatine; D006632:Histamine; D000123:Acetyltransferases; D009955:Ornithine Decarboxylase; D013095:Spermidine; D011700:Putrescine