Expression of CDY1 may identify complete spermatogenesis.
OBJECTIVE
To investigate the expression of deleted in azoospermia (DAZ), RNA-binding motif (RBM), and chromodomain y1 (CDY1) genes in the testes of men with azoospermia with variable histopathologies.


METHODS
Prospective study.


METHODS
Andrology laboratory of a university-affiliated maternity hospital.


METHODS
Sixty-six men with azoospermia.


METHODS
Testicular sperm extraction.


METHODS
The results of gene expression in testicular tissue tested by RT-PCR were correlated with those of histopathologically and microscopically examined minced testicular tissue. Y chromosome microdeletion testing and karyotyping were performed, as was direct sequencing of CDY1-PCR products.


RESULTS
CDY1-minor expression was detected in all biopsies in which mature spermatids/spermatozoa were observed by histological analysis and/or in the minced tissue. CDY1-minor expression was also detected in two biopsies with arrest at the spermatocyte stage during which no mature spermatids/spermatozoa were observed. A previously unreported CDY1-minor alternative splicing transcript was identified. DAZ and RBM gene expressions were detected in all biopsies in which at least a few germinal cells in early stages were found and in one biopsy histologically determined as Sertoli cell only.


CONCLUSIONS
Our preliminary results suggest that CDY1-minor expression might increase the prospect for complete spermatogenesis, while RBM and DAZ expression can only be indicative of the presence of germinal cells.
Kleiman
S E
SE
Institute for the Study of Fertility, Lis Maternity Hospital, Tel Aviv Sourasky Medical Center, The Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel. ser@tasmc.health.gov.il
Lagziel
A
A
Yogev
L
L
Botchan
A
A
Paz
G
G
Yavetz
H
H
D000328:Adult; D002878:Chromosomes, Human, Pair 1; D017353:Gene Deletion; D005786:Gene Expression Regulation / Q000235:genetics / Q000502:physiology; D006801:Humans; D007621:Karyotyping; D007713:Klinefelter Syndrome / Q000235:genetics / Q000473:pathology; D008297:Male; D009845:Oligospermia / Q000235:genetics* / Q000473:pathology; D012313:RNA / Q000032:analysis / Q000096:biosynthesis; D016601:RNA-Binding Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013087:Spermatids / Q000737:chemistry / Q000648:ultrastructure; D013091:Spermatogenesis / Q000235:genetics*; D013737:Testis / Q000166:cytology / Q000473:pathology
D016601:RNA-Binding Proteins; D012313:RNA