Activity of platelet in patients with high level of LDL and the effect of LDL on platelet glycoproteins.
OBJECTIVE
To investigate the activity of platelet in patients with high level of low density lipoproteins (LDL) and the effect of LDL on platelet glycoproteins (GP).


METHODS
Platelet glycoproteins, whose platelet were activated or unactivated, were measured by flow cytometry.


RESULTS
The amount of the platelet membrane GP II b/III a in patients was not significantly different from that of the control when platelet was unactivated (P > 0.05); when platelet was activated by adenosine diphosphate (ADP), the amount of patients' platelet GP II b/III a was increased markedly in comparison with that in the control (P < 0.01). Having been preincubated with LDL, the platelets were activated by ADP and the amounts of GP II b/III a of patients group and the control were all increased obviously as compared with those in the platelets which were not incubated with LDL (P < 0.01; P < 0.01). There was no significant difference of the amount of granule membrane protein-140 (GMP-140) between patients and the control when platelet was activated by thrombin (P > 0.05). Having been preincubated with LDL, platelet was activated by thrombin, the amounts of GMP-140 on patients' and control platelet were all increased markedly (P < 0.01, P < 0.01).


CONCLUSIONS
The activity of platelet in patients with high level of LDL is increased significantly. LDL can increase the expression of platelet glycoproteins.
Zhang
Y
Y
Department of Hematology, Union Hospital, Tongji Medical University, Wuhan 430022, China.
Shen
D
D
Zou
P
P
Wei
W
W
Wang
A
A
Yang
L
L
D000328:Adult; D000368:Aged; D004244:Dizziness / Q000097:blood*; D005260:Female; D006801:Humans; D006951:Hyperlipoproteinemias / Q000097:blood*; D008077:Lipoproteins, LDL / Q000097:blood*; D008297:Male; D008875:Middle Aged; D019007:P-Selectin / Q000097:blood*; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000378:metabolism*
D008077:Lipoproteins, LDL; D019007:P-Selectin; D019039:Platelet Glycoprotein GPIIb-IIIa Complex