Restoration of vancomycin susceptibility in Enterococcus faecalis by antiresistance determinant gene transfer.
We assessed the ability of gene transfer to reverse vancomycin resistance in class A (VanA) glycopeptide-resistant Enterococcus faecalis. Recombinant shuttle vectors containing a vanH promoter-vanA antisense gene cassette fully restored vancomycin susceptibility through a combined transcriptional activator binding domain decoy and inducible vanA antisense RNA effect.
Torres Viera
C
C
Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA.
Tsiodras
S
S
Gold
H S
HS
Coakley
E P
EP
Wennersten
C
C
Eliopoulos
G M
GM
Moellering
R C
RC
Inouye
R T
RT
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors / Q000235:genetics; D019729:Carbon-Oxygen Ligases / Q000037:antagonists & inhibitors / Q000235:genetics; D004347:Drug Interactions; D013293:Enterococcus faecalis / Q000187:drug effects / Q000235:genetics*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000235:genetics / Q000494:pharmacology*; D014169:Transformation, Bacterial; D014640:Vancomycin / Q000494:pharmacology*; D020713:Vancomycin Resistance / Q000235:genetics*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D016376:Oligonucleotides, Antisense; C071437:VanA ligase, Bacteria; D014640:Vancomycin; D019729:Carbon-Oxygen Ligases