BglII and MunI: what a difference a base makes.
Restriction endonucleases are resilient to alterations in their DNA-binding specificities. Structures of the BglII and MunI endonucleases bound to their palindromic DNA sites, which differ by only their outer base pairs from the recognition sequences of BamHI and EcoRI, respectively, have recently been determined. A comparison of these complexes reveals surprising differences and similarities in structure, and provides a basis for understanding the immutability of restriction endonucleases.
Lukacs
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CM
Hoffman-La Roche Inc, 340 Kingsland Street, Nutley, NJ 07110, USA. Christine.lukacs@roche.com
Aggarwal
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AK
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D013379:Substrate Specificity
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C059776:endodeoxyribonuclease MfeI; C024404:BglII endonuclease; D015252:Deoxyribonucleases, Type II Site-Specific