The structural basis for the action of the antibiotics tetracycline, pactamycin, and hygromycin B on the 30S ribosomal subunit.
We have used the recently determined atomic structure of the 30S ribosomal subunit to determine the structures of its complexes with the antibiotics tetracycline, pactamycin, and hygromycin B. The antibiotics bind to discrete sites on the 30S subunit in a manner consistent with much but not all biochemical data. For each of these antibiotics, interactions with the 30S subunit suggest a mechanism for its effects on ribosome function.
Brodersen
D E
DE
MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom.
Clemons
W M
WM
Carter
A P
AP
Morgan-Warren
R J
RJ
Wimberly
B T
BT
Ramakrishnan
V
V
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000737:chemistry; D001665:Binding Sites; D018360:Crystallography, X-Ray; D006921:Hygromycin B / Q000494:pharmacology*; D018716:Molecular Mimicry; D010142:Pactamycin / Q000494:pharmacology*; D017434:Protein Structure, Tertiary; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D012333:RNA, Messenger / Q000737:chemistry; D012343:RNA, Transfer / Q000737:chemistry; D012269:Ribosomal Proteins / Q000737:chemistry; D012270:Ribosomes / Q000737:chemistry / Q000187:drug effects*; D013329:Structure-Activity Relationship; D013752:Tetracycline / Q000494:pharmacology*; D016963:Thermus thermophilus
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; D012269:Ribosomal Proteins; D010142:Pactamycin; D006921:Hygromycin B; D012343:RNA, Transfer; D013752:Tetracycline