Expression of the 17beta-hydroxysteroid dehydrogenase type 5 mRNA in the human brain.
An enzyme-mediated metabolism of androgens and estrogens including 17beta-HSD activity in the brain of vertebrates was discovered approximately 30 years ago. Mainly 5alpha-reductase and aromatase have been studied in detail. Recently we could demonstrate reductive and oxidative 17beta-HSD activity as well as considerable mRNA expression of the 17beta-HSD types 3 and 4 in the human brain. In the present study, we report on 17beta-HSD type 5 mRNA expression in brain tissue of women and men. Data analysis did not reveal sex specific differences, but we determined a significantly higher mRNA concentration in the subcortical white matter (SC) than in the cerebral cortex (CX). Investigation of reductive 17beta-HSD in vitro activity with 2 microM androstenedione as the substrate revealed no sex specific differences. Testosterone formation was significantly higher in SC than in CX. Moreover, enzyme activity was significantly higher in brain tissue of adults compared to that of children.
Steckelbroeck
S
S
Department of Clinical Biochemistry, University of Bonn, Sigmund-Freud-Strasse 25, D-53127, Bonn, Germany. st_steckelbroeck@hotmail.com
Watzka
M
M
Stoffel-Wagner
B
B
Hans
V H
VH
Redel
L
L
Clusmann
H
H
Elger
C E
CE
Bidlingmaier
F
F
Klingmüller
D
D
D015067:17-Hydroxysteroid Dehydrogenases / Q000235:genetics* / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D000368:Aged; D000735:Androstenedione / Q000378:metabolism; D001921:Brain / Q000201:enzymology*; D002540:Cerebral Cortex / Q000201:enzymology; D002648:Child; D002675:Child, Preschool; D005260:Female; D015870:Gene Expression; D006801:Humans; D007223:Infant; D007527:Isoenzymes / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009249:NADP / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis*; D012727:Sex Characteristics; D013702:Temporal Lobe / Q000201:enzymology; D013739:Testosterone / Q000378:metabolism; D014018:Tissue Distribution
D007527:Isoenzymes; D012333:RNA, Messenger; D013739:Testosterone; D000735:Androstenedione; D009249:NADP; D015067:17-Hydroxysteroid Dehydrogenases