Miniaturized tests for computer-assisted identification of motile Aeromonas species with an improved probability matrix.
OBJECTIVE
To develop miniaturized tests for the phenotypic identification of motile Aeromonas species using an improved probability matrix.


RESULTS
Conventional tests were miniaturized for use in 96-well plates, and their performance assessed using 60 aeromonads comprising type and reference strains as well as clinical, fish and water isolates. A revised probability matrix for Aeromonas hybridization groups 1-14, including A. allosaccharophila, A. bestiarum, A. encheleia and A. popoffii, was developed. Using 26 tests, all the reference strains were correctly identified with the revised probability matrix, and 80% of the isolates were correctly identified at a Willcox probability level of 95%.


CONCLUSIONS
The compact test format, coupled with a robust identification matrix, provides a convenient basis for identifying motile aeromonads.


CONCLUSIONS
The identification system for identifying aeromonads will be of use to medical and veterinary laboratories undertaking disease diagnosis.
Carson
J
J
Fish Health Unit, Department of Primary Industries, Water & Environment, PO Box 46, Kings Meadows, Launceton, Tasmania 7249, Australia. jeremy.carson@dpiwe.tas.gov.au
Wagner
T
T
Wilson
T
T
Donachie
L
L
D000333:Aeromonas / Q000145:classification* / Q000302:isolation & purification / Q000502:physiology; D000818:Animals; D015373:Bacterial Typing Techniques; D003198:Computer Simulation; D003470:Culture Media; D005399:Fishes / Q000382:microbiology; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D006801:Humans; D008962:Models, Theoretical; D009068:Movement / Q000502:physiology; D011336:Probability; D015203:Reproducibility of Results
D003470:Culture Media