Virucidal efficacy of a combination of 0.2% peracetic acid and 80% (v/v) ethanol (PAA-ethanol) as a potential hand disinfectant.
The formulation PAA-ethanol, consisting of 0.2% paracetic acid (PAA) and 80% ethanol, was tested for its virucidal activity on the enveloped vaccinia virus and papova virus SV 40 and the non-enveloped adenovirus type 2 and poliovirus type 1. All test viruses were inactivated by PAA-ethanol within an exposure time of 1 minute, as measured by a log(10)reduction of 4 in virus titres. It was shown that poliovirus type 1 can be inactivated by PAA-ethanol within 1 min, whilst for the aqueous 0.2% PAA solution, an exposure time of 5 min is necessary. As shown by electron microscopic observation, the structure of virus particles was completely destroyed by 0.2% PAA within 15 min. The broad antimicrobial spectrum including a high virucidal activity, short exposure time and no toxic or allergenic decomposition products are favourable prerequisites for the medical use of PAA-ethanol and it warrants further investigation as a hand disinfectant.
Wutzler
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Institute for Antiviral Chemotherapy, Friedrich-Schiller University of Jena, Winzelaer Str. 10, 07745 Jena, Germany. wutzler@zmkh.ef.uni-jena.de
Sauerbrei
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D000260:Adenoviruses, Human / Q000187:drug effects* / Q000648:ultrastructure; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D003428:Cross Infection / Q000517:prevention & control / Q000821:virology; D004202:Disinfectants / Q000494:pharmacology*; D004338:Drug Combinations; D004353:Drug Evaluation, Preclinical; D000431:Ethanol / Q000494:pharmacology; D006225:Hand / Q000821:virology*; D006235:Hand Disinfection / Q000379:methods*; D006801:Humans; D008854:Microscopy, Electron; D027383:Papillomaviridae / Q000187:drug effects* / Q000648:ultrastructure; D010463:Peracetic Acid / Q000494:pharmacology*; D017955:Poliovirus / Q000187:drug effects* / Q000648:ultrastructure; D027382:Polyomaviridae; D013997:Time Factors; D014616:Vaccinia virus / Q000187:drug effects* / Q000648:ultrastructure
D000891:Anti-Infective Agents, Local; D004202:Disinfectants; D004338:Drug Combinations; D000431:Ethanol; D010463:Peracetic Acid