Monitoring of Lactobacillus fermentation process by using ion chromatography with a series piezoelectric quartz crystal detector.
A new method monitoring Lactobacillus fermentation process, which combines ion chromatography (IC) with a series piezoelectric quartz crystal (SPQC) technique, is presented in this paper. Monitoring of the fermentation process was realized by examining the rate of production of lactic acid. An automatic membrane dialyser was used for the pretreatment of the sample in on-line monitoring. A mixture of p-hydroxybenzoic acid and N,N-diethylethanolamine was adopted as mobile phase and its flow rate was 0.8 ml/min. The effects of some fermentation conditions were also discussed in detail. Accordingly, the optimal fermentation conditions were obtained. This method is simple and convenient while the results obtained are accurate and reliable.
Zhang
J
J
College of Chemistry and Chemical Engineering, Hunan University, 410082, Changsha, People's Republic of China.
Xie
Y
Y
Dai
X
X
Wei
W
W
D002264:Carboxylic Acids / Q000378:metabolism*; D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods; D003470:Culture Media / Q000737:chemistry; D004566:Electrodes; D005285:Fermentation; D007778:Lactobacillus / Q000378:metabolism*; D011791:Quartz
D002264:Carboxylic Acids; D003470:Culture Media; D011791:Quartz