Cord blood banking: volume reduction using "Procord" Terumo filter.
Cryopreserved cord blood (CB) banking, space storage, and ABO major incompatibility transfusion as well as potential progenitor cell loss during processing, are the subjects of this study. We evaluate processing of fresh and thawed CB on "Procord" (Terumo Corp., Japan). On 16 freshed CBs, mean NC, CD34, CFU-GM yields were, respectively, 54% (SD +/- 20), 75% (SD +/- 25), and 171% (SD +/- 168) in a final volume of 20 ml. Final product was enriched in mononuclear cells (mean 69% granulocytes depletion) with reproducible erythrocyte and platelet depletions means of 97% (SD +/- 1.5) and 93% (SD +/- 8). On seven previous whole frozen CB units, Procord gave comparable red blood cell (98%) depletion with 53% (SD +/- 30) mean CD34 recovery. Procord is an efficient method for erythrocyte depletion of CB, and recoveries of NC and progenitor cells are comparable to those obtained with similar processing. Nevertheless, as all existing methods, it is associated with cell and progenitor cell loss.
Dal Cortivo
L
L
Laboratory of Cellular Therapy, Hopital St. Louis, 1, Avenue Claude, Vellefaux 75010 Paris, France. l.dalcortivo@chu-stlouis.fr
Robert
I
I
Mangin
C
C
Sameshima
T
T
Kora
S
S
Gluckman
E
E
Benbunan
M
M
Marolleau
J P
JP
D018952:Antigens, CD34 / Q000097:blood; D000094902:Blood Banking / Q000379:methods*; D001793:Blood Preservation / Q000379:methods* / Q000592:standards; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods / Q000592:standards; D015925:Cryopreservation / Q000379:methods / Q000592:standards; D005312:Fetal Blood / Q000166:cytology*; D005374:Filtration / Q000295:instrumentation; D006801:Humans; D016130:Immunophenotyping; D007937:Leukapheresis / Q000379:methods / Q000592:standards
D018952:Antigens, CD34