Effects of transfusion on gastrointestinal anastomotic wound healing and leukocyte function in rats.
BACKGROUND
This study was done to investigate the effects of blood transfusion on gastrointestinal anastomotic wound healing and leukocyte function.


METHODS
Closed-colony male Wistar rats were divided into five groups in a group-mixed fashion and underwent laparotomy (the CONT and WB groups) or gastrectomy and gastroduodenostomy (the GAST, GAST/WB and GAST/LDRWB groups). Immediately, they received 3 ml of saline (the CONT and GAST groups) or blood from Wistar donors (the WB and GAST/WB groups, whole blood; the GAST/LDRWB group, a transfusion of irradiated and leukocyte-depleted whole blood). The animals were killed 7 days after surgery.


RESULTS
The GAST/WB group had a higher incidence of anastomotic abscess, a lower level of factor XIII and a lower maximum breaking strength than the GAST group. Gastrectomy decreased L-DCL (luminol-dependent chemiluminescence) more than whole-blood transfusion. In the GAST/WB group, L-DCL was severely reduced, while in the GAST/LDRWB group it was roughly equivalent to the level in the GAST group.


CONCLUSIONS
Whole-blood transfusion increased the incidence of anastomotic abscess and impaired anastomotic wound healing, and these might relate to the reduction of L-DCL. Irradiated and leukocyte-depleted transfusion can improve these effects.
Ohwada
S
S
Second Department of Surgery, Faculty of Medicine, Gunma University, Maebashi, Japan. sohwada@sb.gumma-u.ac.jp
Sato
Y
Y
Sato
N
N
Toyama
Y
Y
Okano
T
T
Nakasone
Y
Y
Ogawa
T
T
Morishita
Y
Y
D000714:Anastomosis, Surgical; D000818:Animals; D001770:Blood Bactericidal Activity; D001777:Blood Coagulation; D004385:Duodenostomy; D005176:Factor XIII / Q000378:metabolism; D005774:Gastrostomy; D006400:Hematocrit; D007202:Indicators and Reagents; D007962:Leukocytes / Q000502:physiology*; D008163:Luminescent Measurements; D008297:Male; D009930:Organic Chemicals; D051381:Rats; D017208:Rats, Wistar; D065227:Transfusion Reaction; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
D007202:Indicators and Reagents; D009930:Organic Chemicals; C013014:hepaplastin; D005176:Factor XIII