Expression of specific markers and particle transport in a new human intestinal M-cell model.
The aim of this work was to establish a new, simplified in vitro model of the human M-cell. Cocultures of physically separated human intestinal epithelial Caco-2 cells and B-cell lymphoma Raji cells were established. The cocultures were characterized under the criteria of morphology, integrity, expression of M-cell markers and cell adhesion molecules (CAMs), and altered particle transport. Using this construct, the epithelial cells were transformed to cells with an M-cell-like morphology and had altered expression of potential human M-cell markers (alkaline phosphatase down-regulation and Sialyl Lewis A antigen up-regulation). The expression of intercellular adhesion molecule-1 and vascular cell adhesion molecule was altered and there was an increased binding of lectins wheat germ agglutinin and peanut agglutinin with a 40-fold increase in microparticle transport. The particle transport was size-dependent and could be inhibited at 4 degrees C or by replacing the Raji B-cells with Jurkat T-cells. This new coculture model will enable controlled studies of M-cell development and function in vitro.
Gullberg
E
E
Division of Pharmaceutics, Uppsala University, Uppsala, Sweden.
Leonard
M
M
Karlsson
J
J
Hopkins
A M
AM
Brayden
D
D
Baird
A W
AW
Artursson
P
P
D000469:Alkaline Phosphatase / Q000096:biosynthesis*; D000951:Antigens, Neoplasm / Q000096:biosynthesis*; D001692:Biological Transport / Q000502:physiology; D018938:Caco-2 Cells; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D048429:Cell Size; D003106:Colon / Q000166:cytology*; D006801:Humans; D008954:Models, Biological
D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; C042317:DU-PAN-2 antigen, human; D000469:Alkaline Phosphatase