Crystallization and preliminary crystallographic studies of an antimicrobial protein from Pharbitis nil.
An antimicrobial protein from seeds of Pharbitis nil (Pn-AMP) which shows an antifungal activity towards several agriculturally important plant pathogens has been crystallized in the presence of equimolar N-acetylglucosamine with sodium citrate as precipitant. The crystal belongs to the hexagonal space group P6(1)22 (or P6(5)22), with unit-cell parameters a = b = 29.33 (5), c = 133.44 (12) A. Native data were collected using a crystal at 100 K to a resolution of 1.78 A.
Ha
S C
SC
Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Chinju 660-701, South Korea.
Min
K
K
Koo
J C
JC
Kim
Y
Y
Yun
D J
DJ
Cho
M J
MJ
Kim
K K
KK
D000595:Amino Acid Sequence; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000302:isolation & purification; D000935:Antifungal Agents / Q000737:chemistry; D003460:Crystallization; D018360:Crystallography, X-Ray; D019684:Magnoliopsida / Q000737:chemistry*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification; D012639:Seeds / Q000737:chemistry; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D000890:Anti-Infective Agents; D000935:Antifungal Agents; D010940:Plant Proteins