Quantitative and qualitative effects of chemical peeling on photo-aged skin: an experimental study.
Chemical peel reverses the visible stigmata of photo aging in human skin. The qualitative and, in particular, the quantitative changes in the dermis that effect this transformation are unclear. This study used a recognized photo-aged animal model, the Skh:HR-1 hairless mouse, to quantify and qualify the changes that occurred in collagen and glycosaminoglycan content after chemical peel. One hundred Skh:HR-1 hairless mice were photo-aged by use of chronic ultraviolet B irradiation for 14 weeks. After irradiation the animals were randomly distributed into five groups of 20 mice each: group 1, control; group 2, 50% glycolic acid peel; group 3, 30% trichloroacetic acid peel; group 4, 50% trichloroacetic acid peel; group 5, phenol peel (Baker-Gordon formula). The respective peeling agent was applied to the dorsal skin of each animal while it was fully anesthetized. Punch biopsies were taken at several times after peel for histological and biochemical analysis. Glycosaminoglycan content was assessed at 14, 28, and 60 days using a colorimetric assay. Collagen content per unit volume increased initially 3 days after the procedure in all chemical peel groups, declining on day 7, and peaking again on day 28. Significant elevations (p < 0.04) were seen in the 30% trichloroacetic acid, 50% trichloroacetic acid, and phenol peels on days 3 and 28 in comparison with controls. This increase in collagen content was not maintained and returned to control values by 60 days. Glycosaminoglycan content per unit volume was elevated initially after peel with significant elevation (p < 0.02) in the 50% trichloroacetic acid and phenol groups on days 14 and 28. This increase in glycosaminoglycan content was not maintained beyond 28 days and declined to control values by day 60 in all groups. Histological examination demonstrated an increase in dermal thickness in the 50% trichloroacetic acid and phenol groups in comparison with controls by day 60. Under polarized light all chemical peel groups at day 60 demonstrated a reorganization of collagen in the reticular and papillary dermis. The elastotic masses that are pathognomonic of photo aging were present in the control group but were absent in the peel groups and demonstrated a reorganization of the elastic fibers in the dermis. This effect was deeper in the dermis in the deeper peel groups (50% trichloroacetic acid and phenol peel). The beneficial effects of chemical peel were due to a combination of two findings; a reorganization in dermal structural elements and an increase in dermal volume. These effects were more pronounced in the deeper peel groups.
Butler
P E
PE
Division of Plastic Surgery, Massachusetts General Hospital, Harvard Medical School, Boston 02114, USA.
Gonzalez
S
S
Randolph
M A
MA
Kim
J
J
Kollias
N
N
Yaremchuk
M J
MJ
D000818:Animals; D002615:Chemexfoliation; D003094:Collagen; D006016:Glycolates; D006025:Glycosaminoglycans / Q000032:analysis; D051379:Mice; D008812:Mice, Hairless; D019800:Phenol; D012867:Skin / Q000737:chemistry / Q000473:pathology*; D015595:Skin Aging / Q000187:drug effects / Q000473:pathology; D014238:Trichloroacetic Acid; D014466:Ultraviolet Rays
D006016:Glycolates; D006025:Glycosaminoglycans; C031149:glycolic acid; D019800:Phenol; D014238:Trichloroacetic Acid; D003094:Collagen