title: Expression of adhesion and activation molecules in human buccal epithelial cell lines and normal human buccal epithelium in situ.
abstract: An in vitro culture system may be used to analyse interactions between T cells and epithelium. We have analysed two human buccal squamous epithelial cell lines: SqCC/Y1, derived from a buccal carcinoma, and SVpgC2a, an SV-transformed healthy buccal squamous epithelium. Under serum-free culture conditions, the cell lines resemble normal buccal squamous epithelium in situ in their expression of MHC class I, CD29 (beta1 integrin), CD40, CD44, CD54 (ICAM-1), CD58 (LFA-3), CD95 (Fas), and E-cadherin. Furthermore, when the SVpgC2a cell line was treated with T-cell derived cytokines, upregulation of CD40 expression was observed when the cells were treated with IL-4, whereas IFN-gamma caused upregulation in expression of CD40, CD54 and MHC class II. These buccal squamous epithelial cell lines, therefore, provide a good tool for analysing interactions between epithelium and T cells in vitro.
authors.affiliation: Department of Oral Medicine, Leeds Dental Institute, UK.
authors.forename: I
authors.initials: I
authors.lastname: Farmer
authors.forename: J
authors.initials: J
authors.lastname: Freysdottir
authors.forename: A M
authors.initials: AM
authors.lastname: Dalghous
authors.forename: F
authors.initials: F
authors.lastname: Fortune
mesh_terms: D019013:CD40 Antigens / Q000032:analysis; D018968:CD58 Antigens / Q000032:analysis; D015820:Cadherins / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000032:analysis* / Q000235:genetics; D002460:Cell Line; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D016895:Culture Media, Serum-Free; D016207:Cytokines / Q000494:pharmacology; D004847:Epithelial Cells / Q000166:cytology / Q000473:pathology; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D015395:Histocompatibility Antigens Class I / Q000032:analysis; D006801:Humans; D018960:Hyaluronan Receptors / Q000032:analysis; D019012:Integrin beta1 / Q000032:analysis; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D007371:Interferon-gamma / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology; D009061:Mouth Mucosa / Q000166:cytology* / Q000473:pathology; D009062:Mouth Neoplasms / Q000473:pathology; D013539:Simian virus 40; D014407:Tumor Cells, Cultured; D015854:Up-Regulation; D019014:fas Receptor / Q000032:analysis
chemical_list: D019013:CD40 Antigens; D018968:CD58 Antigens; D015820:Cadherins; D015815:Cell Adhesion Molecules; D016895:Culture Media, Serum-Free; D016207:Cytokines; D015395:Histocompatibility Antigens Class I; D018960:Hyaluronan Receptors; D019012:Integrin beta1; D019014:fas Receptor; D018799:Intercellular Adhesion Molecule-1; D015847:Interleukin-4; D007371:Interferon-gamma