title: In vitro and in vivo infectivity and pathogenicity of the lymphoid cell-derived woodchuck hepatitis virus.
abstract: Woodchuck hepatitis virus (WHV) and human hepatitis B virus are closely related, highly hepatotropic mammalian DNA viruses that also replicate in the lymphatic system. The infectivity and pathogenicity of hepadnaviruses propagating in lymphoid cells are under debate. In this study, hepato- and lymphotropism of WHV produced by naturally infected lymphoid cells was examined in specifically established woodchuck hepatocyte and lymphoid cell cultures and coculture systems, and virus pathogenicity was tested in susceptible animals. Applying PCR-based assays discriminating between the total pool of WHV genomes and covalently closed circular DNA (cccDNA), combined with enzymatic elimination of extracellular viral sequences potentially associated with the cell surface, our study documents that virus replicating in woodchuck lymphoid cells is infectious to homologous hepatocytes and lymphoid cells in vitro. The productive replication of WHV from lymphoid cells in cultured hepatocytes was evidenced by the appearance of virus-specific DNA, cccDNA, and antigens, transmissibility of the virus through multiple passages in hepatocyte cultures, and the ability of the passaged virus to infect virus-naive animals. The data also revealed that WHV from lymphoid cells can initiate classical acute viral hepatitis in susceptible animals, albeit small quantities (approximately 10(3) virions) caused immunovirologically undetectable (occult) WHV infection that engaged the lymphatic system but not the liver. Our results provide direct in vitro and in vivo evidence that lymphoid cells in the infected host support propagation of infectious hepadnavirus that has the potential to induce hepatitis. They also emphasize a principal role of the lymphatic system in the maintenance and dissemination of hepadnavirus infection, particularly when infection is induced by low virus doses.
authors.affiliation: Molecular Virology and Hepatology Research, Division of Basic Medical Science, Faculty of Medicine, Health Sciences Centre, Memorial University of Newfoundland, St. John's, Newfoundland A1B 3V6, Canada.
authors.forename: Y Y
authors.initials: YY
authors.lastname: Lew
authors.forename: T I
authors.initials: TI
authors.lastname: Michalak
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004270:DNA, Circular / Q000032:analysis / Q000097:blood; D004279:DNA, Viral / Q000032:analysis / Q000097:blood; D006509:Hepatitis B / Q000635:transmission / Q000821:virology*; D017919:Hepatitis B Virus, Woodchuck / Q000472:pathogenicity* / Q000502:physiology; D022781:Hepatocytes / Q000166:cytology / Q000821:virology; D008214:Lymphocytes / Q000821:virology*; D008392:Marmota; D012692:Serial Passage; D014779:Virus Replication
chemical_list: D004270:DNA, Circular; D004279:DNA, Viral