Chlamydia outer membrane protein discovery using genomics.
Outer membrane proteins of microbial pathogens serve essential roles in engaging the host environment and can be important immunotherapeutic targets. Because of the difficulty of growing large quantities of chlamydiae suitable for biochemical fractionation, little was known about their outer membrane protein composition prior to the recent sequencing of the C. trachomatis and C. pneumoniae genomes. Using bioinformatic approaches to characterize chlamydial open reading frames, novel outer membrane proteins were predicted. Several of the predicted outer membrane proteins recently have been shown to be translated and localized to the surface of the chlamydial outer membrane.
Stephens
R S
RS
Division of Infectious Diseases, School of Public Health, University of California, Berkeley, California 94720, USA.
Lammel
C J
CJ
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001426:Bacterial Proteins; D002689:Chlamydia / Q000235:genetics*; D002692:Chlamydia trachomatis / Q000235:genetics; D016993:Chlamydophila pneumoniae / Q000235:genetics; D016680:Genome, Bacterial; D011110:Polymorphism, Genetic; D018272:Porins / Q000235:genetics
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; C115176:Omp85 protein, Neisseria; C419549:PorB protein, Chlamydia trachomatis; D018272:Porins