title: Degradation of insulin-like growth factors in small intestine of suckling rats.
abstract: Insulin-like growth factors (IFGs), IGF-I and IGF-II, present in mammalian milk, play an important role during gastrointestinal tract development. In this study we identified and localized the activities of the common intestinal proteolytic enzymes and investigated their degradation effect on IGFs. Results indicated that the enzymatic activities of chymotrypsin, trypsin, and elastase progressed from the lowest in the duodenum, to the highest in the midjejunum, and declined in the ileum. Chymotrypsin exhibited the greatest IGFs degradation activities in neonatal intestinal lumen followed by elastase. These data furnish a potential strategic design to supplement IGFs into milk formulas.
authors.affiliation: Department of Pediatrics and Steele Memorial Children's Research Center, University of Arizona, 1501 N. Campbell Avenue, PO Box 245073, Tucson, AZ 85724, USA.
authors.forename: A M
authors.initials: AM
authors.lastname: Fellah
authors.forename: A F
authors.initials: AF
authors.lastname: Philipps
authors.forename: T J
authors.initials: TJ
authors.lastname: Gillespie
authors.forename: J R
authors.initials: JR
authors.lastname: Galo
authors.forename: B
authors.initials: B
authors.lastname: Dvorák
mesh_terms: D000818:Animals; D000833:Animals, Suckling; D002918:Chymotrypsin / Q000378:metabolism; D004798:Enzymes / Q000378:metabolism*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D007335:Insulin-Like Growth Factor II / Q000378:metabolism*; D007421:Intestine, Small / Q000378:metabolism*; D007457:Iodine Radioisotopes; D010196:Pancreatic Elastase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014357:Trypsin / Q000378:metabolism
chemical_list: D004798:Enzymes; D007457:Iodine Radioisotopes; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II; D002918:Chymotrypsin; D010196:Pancreatic Elastase; D014357:Trypsin