title: Identification of two proteins, S14 and UIP1, that interact with UCH37.
abstract: By the use of the yeast two-hybrid screen we have identified two proteins that interacted with UCH37: S14, which is a subunit of PA700 and a novel protein, UIP1 (UCH37 interacting protein 1). The interaction of UCH37 with S14 or UIP1 was confirmed by in vitro binding assay and in vivo co-immunoprecipitation analysis. The C-terminal extension of UCH37 is essential for interaction with S14 or UIP1 as shown by the yeast two-hybrid assay and the in vitro binding assay. Furthermore, UIP1 blocked the interaction between UCH37 and S14 in vitro.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, 119260, Singapore, Singapore.
authors.forename: T
authors.initials: T
authors.lastname: Li
authors.forename: W
authors.initials: W
authors.lastname: Duan
authors.forename: H
authors.initials: H
authors.lastname: Yang
authors.forename: M K
authors.initials: MK
authors.lastname: Lee
authors.forename: F
authors.initials: F
authors.lastname: Bte Mustafa
authors.forename: B H
authors.initials: BH
authors.lastname: Lee
authors.forename: T S
authors.initials: TS
authors.lastname: Teo
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D001667:Binding, Competitive; D015153:Blotting, Western; D002268:Carboxypeptidases; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D006801:Humans; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D046988:Proteasome Endopeptidase Complex; D011485:Protein Binding; D021122:Protein Subunits; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D016415:Sequence Alignment; D013869:Thiolester Hydrolases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D043222:Ubiquitin Thiolesterase
chemical_list: D002352:Carrier Proteins; C085834:PA700 proteasome activator; D021122:Protein Subunits; D011506:Proteins; D012333:RNA, Messenger; D013869:Thiolester Hydrolases; D002268:Carboxypeptidases; C421245:UCHL5 protein, human; D043222:Ubiquitin Thiolesterase; D046988:Proteasome Endopeptidase Complex