Diversity of Helicobacter pylori among Chinese persons with H. pylori infection.
To determine whether there is diversity among clinical isolates of Helicobacter pylori in Chinese patients with peptic ulcer disease, 40 strains of H. pylori were isolated from antral biopsy specimens obtained at the gastroenterology clinic of Xiangya Hospital from January 1996 to June 1998. Total protein profile by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and DNA diversity by polymerase chain reaction-random amplified polymorphic DNA (PCR-RAPD) fingerprinting were performed with these isolates. All the isolates from peptic ulcer disease were relatively homogeneous in protein profiles, but they showed a great DNA sequence diversity by PCR-RAPD fingerprinting. In Chinese patients H. pylori demonstrated an enormous diversity. The diversity among clinical isolates of H. pylori could be distinctly demonstrated and this observation will be helpful in the management of intrafamilial and recurrent H. pylori infection. PCR-RAPD fingerprinting is an efficient method of distinguishing between clinical isolates of H. pylori.
Yakoob
J
J
Department of Infectious Diseases, Xiangya Hospital, Hunan Medical University, Changsha, PR China. yakoobjaved@hotmail.com
Hu
G L
GL
Fan
X G
XG
Yang
H X
HX
Liu
S H
SH
Tan
D M
DM
Li
T G
TG
Zhang
Z
Z
D000328:Adult; D001426:Bacterial Proteins / Q000032:analysis; D002681:China; D016172:DNA Fingerprinting; D004381:Duodenal Ulcer / Q000382:microbiology* / Q000473:pathology; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005260:Female; D005773:Gastroscopy / Q000379:methods; D014644:Genetic Variation; D016481:Helicobacter Infections / Q000175:diagnosis / Q000382:microbiology* / Q000473:pathology; D016480:Helicobacter pylori / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D019105:Random Amplified Polymorphic DNA Technique; D015203:Reproducibility of Results; D012967:Sodium Dodecyl Sulfate; D013276:Stomach Ulcer / Q000382:microbiology* / Q000473:pathology
D001426:Bacterial Proteins; D012967:Sodium Dodecyl Sulfate