Partial characterization of SUVi, a new mammalian gene induced by UV-C and expressed during the S phase of the cell cycle.
By using a lacZ-based gene-trap approach, we identified a mammalian gene induced by UV-C in a Chinese hamster ovary cell clone (Menichini P et al. [1997]: Nucleic Acids Res 25:4803-4807). The activity of the encoded protein fused to a bacterial beta-galactosidase was followed through the hydrolysis of different beta-galactosidase substrates. In this study we describe how the expression of this gene is modulated during the cell cycle and in response to UV-irradiation. We show that the beta-galactosidase activity was virtually undetectable in quiescent cells (G[0]), started to increase when cells progressed in G(1), and reached a maximum in mid-S phase, indicating a possible role of the endogenous protein during DNA synthesis. Following UV-irradiation, besides a delay of the progression through the S phase, a twofold increase of the reporter protein activity in all phases of the cell cycle was observed. The partial sequence analysis showed that this gene, here named SUVi (for S phase UV-inducible), contains a domain that is highly conserved among different helicases. Together, these data suggest that the SUVi gene could be involved in DNA synthesis, a process that takes place both in the S phase and in the processing of UV-induced damage.
Viaggi
S
S
Department of Oncology, Biology, and Genetics, University of Genoa, Italy.
Gallerani
E
E
Molina
F
F
Nuesse
M
M
Fronza
G
G
Ottaggio
L
L
Campomenosi
P
P
Abbondandolo
A
A
Menichini
P
P
D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002453:Cell Cycle / Q000235:genetics*; D018797:Cell Cycle Proteins; D002460:Cell Line; D002999:Clone Cells / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D003001:Cloning, Molecular; D006224:Cricetinae; D004265:DNA Helicases / Q000096:biosynthesis / Q000235:genetics*; D004260:DNA Repair; D017930:Genes, Reporter / Q000235:genetics / Q000528:radiation effects; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017434:Protein Structure, Tertiary / Q000235:genetics; D012313:RNA / Q000235:genetics; D016196:S Phase / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014466:Ultraviolet Rays; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects
D018797:Cell Cycle Proteins; C421222:SUVi protein, Cricetulus griseus; D012313:RNA; D001616:beta-Galactosidase; D004265:DNA Helicases