Arrayed transposase-binding sequences on the ends of transposon Tn5090/Tn402.
The transposon Tn5090/Tn402 encodes a 559 amino acid transposase, TniA, with a DDE motif. Gel mobility shifting and cleavage protection analysis with DNase I and hydroxyl radical probes revealed that TniA binds to multiple repeat sequences on either terminus of Tn5090/Tn402. Four of these TniA-binding 19mers occurred on the left-hand (t) end and two on the right-hand (i) end. Hydroxyl radical cleavage protection demonstrated the presence of 3-6 bp contact sequences on one face of the DNA helix. The binding pattern and organisation of repeats suggested parallels between Tn5090/Tn402 and Mu, which controls its transpositional activity in the assembly step of a higher order transpososome complex. The complex terminal structure and genes of transposase and nucleotide-binding proteins in tandem are hallmarks of the handful of Mu-like elements that are known to date.
Kamali-Moghaddam
M
M
Department of Pharmaceutical Biosciences, Division of Microbiology, Uppsala University, PO Box 581 BMC, S-751 23 Uppsala, Sweden.
Sundström
L
L
D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D018983:DNA Footprinting; D004251:DNA Transposable Elements / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000378:metabolism; D017665:Hydroxyl Radical / Q000378:metabolism; D008957:Models, Genetic; D008969:Molecular Sequence Data; D011485:Protein Binding; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D019895:Transposases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; D017665:Hydroxyl Radical; D004247:DNA; D019895:Transposases; D003850:Deoxyribonuclease I