The adaptive regulation of amino acid transport system A is associated to changes in ATA2 expression.
The activity of transport system A for neutral amino acids is adaptively stimulated upon amino acid starvation. In cultured human fibroblasts this treatment causes an increase in the expression of the ATA2 system A transporter gene. ATA2 mRNA increase and transport stimulation are suppressed by system A substrates, but they are unaffected by other amino acids. Supplementation of amino acid-starved cells with substrates of system A causes a decrease in both ATA2 mRNA and system A transport activity. These results suggest a direct relationship between ATA2 expression and system A transport activity.
Gazzola
R F
RF
Department of Experimental Medicine, University of Parma, Via Volturno 39, 43100 Parma, Italy.
Sala
R
R
Bussolati
O
O
Visigalli
R
R
Dall'Asta
V
V
Ganapathy
V
V
Gazzola
G C
GC
D026905:Amino Acid Transport Systems; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000096:biosynthesis*; D002460:Cell Line; D003470:Culture Media / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D011392:Proline / Q000378:metabolism; D021381:Protein Transport; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors
D026905:Amino Acid Transport Systems; D002352:Carrier Proteins; D003470:Culture Media; D012333:RNA, Messenger; D011392:Proline