title: High-yield expression of a viral peptide vaccine in transgenic plants.
abstract: A high-yield production of a peptide vaccine in transgenic plants is described here. A 21-mer peptide, which confers protection to dogs against challenge with virulent canine parvovirus, has been expressed in transgenic plants as an amino-terminal translational fusion with the GUS gene. Transformants were selected on the basis of their GUS activities, showing expression levels of the recombinant protein up to 3% of the total leaf soluble protein, a production yield comparable to that obtained with the same epitope expressed by chimeric plant viruses. The immunogenicity of the plant-derived peptide was demonstrated in mice immunized either intraperitoneally or orally with transgenic plant extracts, providing the suitability of the GUS fusions approach for low-cost production of peptide vaccines.
authors.affiliation: Departamento de Mejora Genética y Biotecnología, Madrid, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Gil
authors.forename: A
authors.initials: A
authors.lastname: Brun
authors.forename: A
authors.initials: A
authors.lastname: Wigdorovitz
authors.forename: R
authors.initials: R
authors.lastname: Catalá
authors.forename: J L
authors.initials: JL
authors.lastname: Martínez-Torrecuadrada
authors.forename: I
authors.initials: I
authors.lastname: Casal
authors.forename: J
authors.initials: J
authors.lastname: Salinas
authors.forename: M V
authors.initials: MV
authors.lastname: Borca
authors.forename: J M
authors.initials: JM
authors.lastname: Escribano
mesh_terms: D000284:Administration, Oral; D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000276:immunology; D001483:Base Sequence; D004285:Dogs; D000939:Epitopes / Q000276:immunology; D017930:Genes, Reporter; D005818:Genetic Engineering; D005822:Genetic Vectors / Q000235:genetics; D007274:Injections, Intraperitoneal; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D017992:Parvovirus / Q000276:immunology*; D010455:Peptides / Q000276:immunology; D010944:Plants / Q000235:genetics*; D030821:Plants, Genetically Modified; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000096:biosynthesis / Q000276:immunology; D014170:Transformation, Genetic; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D000939:Epitopes; D010455:Peptides; D011993:Recombinant Fusion Proteins; D022223:Vaccines, Subunit; D014765:Viral Vaccines