Selection of continuous epitope sequences and their incorporation into poly(ethylene glycol)-peptide conjugates for use in serodiagnostic immunoassays: application to Lyme disease.
Continuous epitope sequences were selected from immunogenic Bb proteins by epitope mapping. The identified epitope sequences were synthesized by solid phase peptide synthesis and purified by high performance liquid chromatography. Each epitope was conjugated individually to a multifunctional poly(ethylene glycol) (PEG) carrier. The result PEG-peptide conjugates were used as antigens in ELISA for diagnosis of Lyme disease. The results showed that the defined epitope peptides were Lyme disease specific and could be used in a format of PEG-peptide conjugate as the antigen to achieve improved sensitivity and specificity.
Qiu
B
B
Center for Advanced Biotechnology and Medicine, 679 Hoes Lane, Piscataway, NJ 08854, USA.
Brunner
M
M
Zhang
G
G
Sigal
L
L
Stein
S
S
D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000737:chemistry / Q000235:genetics; D015748:Borrelia burgdorferi Group / Q000235:genetics / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D018796:Immunoconjugates / Q000737:chemistry; D008193:Lyme Disease / Q000175:diagnosis* / Q000276:immunology*; D008969:Molecular Sequence Data; D011092:Polyethylene Glycols; D012698:Serologic Tests
D000942:Antigens, Bacterial; D000939:Epitopes; D018796:Immunoconjugates; D011092:Polyethylene Glycols