Caffeine and theobromine in epicuticular wax of Ilex paraguariensis A. St.-Hil.
Caffeine and theobromine were identified and quantified in leaf epicuticular waxes of Ilex paraguariensis A. St.-Hil. (Aquifoliaceae). The total epicuticular leaf wax content was ca. 0.5% on average of dry leaf weight. Epicuticular caffeine and theobromine contents varied from 0.16 to 127.6 microg/mg and from 0 to 9.5 microg/mg of wax, respectively. For some selected samples, the intracellular methylxanthine concentration was also determined. A positive correlation was found between inner and epicuticular caffeine contents.
Athayde
M L
ML
Departamento de Farmácia Industrial, Universidade Federal de Santa Maria, RS, Brazil.
Coelho
G C
GC
Schenkel
E P
EP
D002110:Caffeine / Q000737:chemistry / Q000302:isolation & purification*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D013056:Spectrophotometry, Ultraviolet; D013805:Theobromine / Q000737:chemistry / Q000302:isolation & purification*
D002110:Caffeine; D013805:Theobromine