title: Characterization at the single-cell level of naive and primed CD8 T cell cytokine responses.
abstract: The aim of this study was to characterize differences between naive and primed CD8 T cells. Our results show that (i) naive and primed CD8 T cells display similar activation thresholds, with no direct evidence for a difference in their TCR signals, and (ii) primed cells differ mainly in their capacity to secrete IFN-gamma. A comparison of the two populations at the single-cell level demonstrated that the increased production of IFN-gamma by the primed cell subset is due to a larger proportion of single cells that are able to synthesize this cytokine early following activation. These results indicate that the intrinsic effector capabilities of individual CD8 T cells expressing the same TCR are heterogeneous and that cells with identical antigen specificity but increased effector capacities are generated or selected during the primary response.
authors.affiliation: Immunologie Apoptose, CERVI, INSERM U503, 21, Avenue Tony Garnies, 69007 Lyon, France.
authors.forename: T
authors.initials: T
authors.lastname: Walzer
authors.forename: G
authors.initials: G
authors.lastname: Joubert
authors.forename: P M
authors.initials: PM
authors.lastname: Dubois
authors.forename: M
authors.initials: M
authors.lastname: Tomkowiak
authors.forename: C
authors.initials: C
authors.lastname: Arpin
authors.forename: M
authors.initials: M
authors.lastname: Pihlgren
authors.forename: J
authors.initials: J
authors.lastname: Marvel
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D016827:CD8 Antigens / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism*; D020013:Calcium Signaling; D002478:Cells, Cultured; D005434:Flow Cytometry; D007114:Immunization; D007156:Immunologic Memory / Q000276:immunology*; D009980:Influenza A virus / Q000276:immunology; D007371:Interferon-gamma / Q000096:biosynthesis / Q000378:metabolism*; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000096:biosynthesis / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D020239:Receptor Cross-Talk; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*
chemical_list: D000956:Antigens, Viral; D016827:CD8 Antigens; D008222:Lymphokines; D011948:Receptors, Antigen, T-Cell; D007371:Interferon-gamma; D011505:Protein-Tyrosine Kinases