title: Distribution of transcripts for the brain-specific GDP/GTP exchange factor collybistin in the developing mouse brain.
abstract: The dbl-like GDP/GTP exchange factor, collybistin, binds to the receptor anchoring protein gephyrin and activates the Rho-like GTPase Cdc42. Collybistin was found in two splice variants I and II, both of which share a tandem Dbl homology/pleckstrin homology (DH/PH) domain. In heterologous expression systems, collybistin II induces the formation of submembraneous gephyrin aggregates and therefore has been implicated in inhibitory synapse formation. Expression of collybistin is restricted to neuronal tissues and is predominantly found in brain. Here, we investigated the spatio-temporal distribution of collybistin transcripts in the embryonic mouse brain and compared it to gephyrin and glycine receptor mRNA patterns. Our data show that collybistin expression is upregulated when neurons become postmitotic and start to differentiate.
authors.affiliation: Department of Neurochemistry, Max-Planck-Institute for Brain Research, Deutschordenstrasse 46, D-60528 Frankfurt/Main, Germany. kneussel@mpih-frankfurt.mpg.de
authors.forename: M
authors.initials: M
authors.lastname: Kneussel
authors.forename: D
authors.initials: D
authors.lastname: Engelkamp
authors.forename: H
authors.initials: H
authors.lastname: Betz
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D002531:Cerebellum / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D002540:Cerebral Cortex / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D005727:Ganglia, Spinal / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics* / Q000378:metabolism; D006153:Guanosine Diphosphate / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D017403:In Situ Hybridization; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D009830:Olfactory Bulb / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D011963:Receptors, GABA-A / Q000378:metabolism; D018009:Receptors, Glycine / Q000235:genetics; D064067:Rho Guanine Nucleotide Exchange Factors; D013116:Spinal Cord / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology; D013569:Synapses / Q000378:metabolism; D018909:src Homology Domains / Q000502:physiology
chemical_list: C577403:Arhgef9 protein, mouse; D002352:Carrier Proteins; D020662:Guanine Nucleotide Exchange Factors; D008565:Membrane Proteins; D012333:RNA, Messenger; D011963:Receptors, GABA-A; D018009:Receptors, Glycine; D064067:Rho Guanine Nucleotide Exchange Factors; C073441:gephyrin; D006153:Guanosine Diphosphate; D006160:Guanosine Triphosphate