Expression profiling in reference bacteria: dreams and reality.
Profiling of gene expression in bacteria is now being used to uncover unknown genes expressed in particular genetic backgrounds or environmental conditions. Obtaining the best possible information from the expected avalanche of such experiments will require standardization of both experimental approach and statistical analysis. The first such experiments reveal challenges, pitfalls and reasonable solutions.
Danchin
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Pasteur Research Centre, Hong Kong University, 8 Sassoon Road, Pokfulam, Hong Kong. adanchin@hkucc.hku.hk
Sekowska
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D001412:Bacillus subtilis / Q000235:genetics / Q000502:physiology; D001419:Bacteria / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics / Q000502:physiology; D020869:Gene Expression Profiling; D016680:Genome, Bacterial; D008954:Models, Biological; D012329:RNA, Bacterial / Q000032:analysis / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics
D012329:RNA, Bacterial