cDNA detection and analysis.
In the continuing search for a full-length cDNA cloning method, there is no clear winner. Perfecting these techniques may require the re-engineering of reverse transcriptase. There now exist two reasonably linear methods for deriving expression signatures from small amounts of biological material, but advances in serial analysis of gene expression provide a quantitative, if expensive, alternative to these methods.
Bashiardes
S
S
Department of Genetics, Washington University School of Medicine, Campus Box 8232, 4566 Scott Avenue, St Louis, Missouri 63110-1093, USA.
Lovett
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M
D000818:Animals; D003001:Cloning, Molecular / Q000379:methods*; D018076:DNA, Complementary / Q000032:analysis / Q000138:chemical synthesis*; D020224:Expressed Sequence Tags; D006801:Humans
D018076:DNA, Complementary