Antibodies against IFN gamma-binding proteins recognize a member of IFN alpha R complex.
Recombinant human IFN alpha 2b coupled to a silica support was used for the purification of the IFN alpha-binding proteins from placental cell membrane extracts. The 100-kDa (p100) and 64-kDa (p64) proteins, which bind preferentially to an IFN alpha 2b-silica matrix, were identified. Using a monoclonal antibody (A6) against IFN-gammaR1, it was able to isolate p100 and p70, but only if IFN alpha 2b was present during chromatography. Similar interactions were observed using polyclonal antibody anti-IFN gamma binding proteins, as assayed in Western blot. These interactions were identified as conformation dependent. We speculate that IFN alpha 2b receptor complex shares an IFN gamma receptor complex epitope.
Bello
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Center for Biological Research, Havana, Cuba. iraldo.bello@cigb.edu.cu
Rodes
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Saura
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D000911:Antibodies, Monoclonal / Q000378:metabolism; D015153:Blotting, Western; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology*; D008565:Membrane Proteins; D010920:Placenta / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D053633:Receptor, Interferon alpha-beta; D017471:Receptors, Interferon / Q000737:chemistry* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism*; D012822:Silicon Dioxide / Q000737:chemistry
D000911:Antibodies, Monoclonal; D000939:Epitopes; D008565:Membrane Proteins; D017471:Receptors, Interferon; D011994:Recombinant Proteins; D053633:Receptor, Interferon alpha-beta; D012822:Silicon Dioxide; D007371:Interferon-gamma