Identification and molecular characterization of an efflux system involved in Pseudomonas putida S12 multidrug resistance.
The authors previously described srpABC, an operon involved in proton-dependent solvent efflux in the solvent-tolerant Pseudomonas putida S12. Recently, it was shown that organic solvents and not antibiotics induce this operon. In the present study, the authors characterize a new efflux pump, designated ArpABC, on the basis of two isolated chloramphenicol-sensitive transposon mutants. The arpABC operon is involved in the active efflux of multiple antibiotics, such as tetracycline, chloramphenicol, carbenicillin, streptomycin, erythromycin and novobiocin. The deduced amino acid sequences encoded by the three genes involved show a striking resemblance to proteins of the resistance/nodulation/cell division family, which are involved in both organic solvent and multiple drug efflux. These findings demonstrate that ArpABC is highly homologous to the MepABC and TtgABC efflux systems for organic solvents and multiple antibiotics. However, ArpABC does not contribute to organic solvent tolerance in P. putida S12 but is solely involved in multidrug resistance.
Kieboom
J
J
Division of Industrial Microbiology, Department of Food Technology and Nutritional Sciences, Wageningen Agricultural University, PO Box 8129, 6700 EV Wageningen, The Netherlands.
de Bont
J
J
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D001555:Benzene Derivatives / Q000494:pharmacology; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002701:Chloramphenicol / Q000494:pharmacology*; D002702:Chloramphenicol Resistance / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D018432:Drug Resistance, Multiple / Q000235:genetics; D005816:Genetic Complementation Test; D026901:Membrane Transport Proteins; D008826:Microbial Sensitivity Tests / Q000379:methods; D008969:Molecular Sequence Data; D009154:Mutation; D011401:Promoter Regions, Genetic; D016958:Pseudomonas putida / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D012997:Solvents / Q000494:pharmacology
D000900:Anti-Bacterial Agents; C435183:ArpA protein, Pseudomonas putida; C435184:ArpB protein, Pseudomonas putida; C435185:ArpC protein, Pseudomonas putida; D001426:Bacterial Proteins; D001555:Benzene Derivatives; D002352:Carrier Proteins; D004251:DNA Transposable Elements; D026901:Membrane Transport Proteins; D012997:Solvents; D002701:Chloramphenicol