Experimental thromboembolic stroke in cynomolgus monkey.
To develop an experimental model of thromboembolic stroke without intracranial surgery, an autologous blood clot was delivered to the middle cerebral artery (MCA) via the internal carotid artery in cynomolgus monkeys. Male cynomolgus monkeys, in which a chronic catheter had been earlier implanted in the left internal carotid artery, were used. The clot was flushed into the internal carotid artery under sevofluorane anesthesia. A neurologic deficit score was assigned after MCA embolization. After 24 h, cerebral infarct size and location were determined by the TTC staining method. Cerebral blood flow (CBF) was measured prior to and after MCA embolization, using positron emission tomography (PET). After embolization, long-lasting and profound extensor hypotonia of the contralateral upper and lower limbs, and mild to severe incoordination were observed. Contralateral hemiplegia was observed over the following 24 h. In gross morphologic observation of the brain, the lesions involved mostly the caudate nucleus, putamen, globus pallidus and insular cortex. CBF was maximally reduced in the left MCA territory, but not in the right MCA territory. This model is relevant to thromboembolic stroke in human in neurologic dysfunction and histopathologic brain damage.
Kito
G
G
Shin Nippon Biomedical Laboratories (SNBL) Ltd., 2438 Miyanoura, Yoshida, 891-1394, Kagoshima, Japan. kito@snbl.co.jp
Nishimura
A
A
Susumu
T
T
Nagata
R
R
Kuge
Y
Y
Yokota
C
C
Minematsu
K
K
D000818:Animals; D001921:Brain / Q000473:pathology / Q000503:physiopathology*; D002560:Cerebrovascular Circulation / Q000502:physiology; D004195:Disease Models, Animal; D020244:Infarction, Middle Cerebral Artery / Q000473:pathology / Q000503:physiopathology*; D008251:Macaca / Q000033:anatomy & histology / Q000293:injuries* / Q000502:physiology; D008297:Male; D020768:Middle Cerebral Artery / Q000473:pathology / Q000503:physiopathology / Q000601:surgery; D013923:Thromboembolism / Q000473:pathology / Q000503:physiopathology*; D014656:Vascular Surgical Procedures / Q000379:methods*