title: [Orthogonal design-direct analysis for PCR optimization].
abstract: Polymerase chain reaction (PCR) optimization is difficult but very important. It is hard to gain the optimal condition due to the confusion of too many factors influencing the experiment. We report a new method, orthogonal design-direct analysis, which has been confirmed to be scientific and easy to follow by many times of PCR.
authors.affiliation: Department of Neurosurgery, Xiangya Hospital, Hunan Medical University, Changsha 410008.
authors.forename: Z
authors.initials: Z
authors.lastname: He
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: L
authors.initials: L
authors.lastname: Chen
authors.forename: M
authors.initials: M
authors.lastname: Cao
authors.forename: J
authors.initials: J
authors.lastname: Xia
mesh_terms: D001932:Brain Neoplasms / Q000235:genetics*; D005091:Exons; D019942:Genes, p16; D005910:Glioma / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*