Functional activity of hepatocytes in tissue fragments in a new bioreactor biological artificial liver.
Functional activity of hepatocytes in a new bioreactor designed for culturing of liver tissue fragments under perfusion conditions was tested. Specific hepatic functions such as ammonium detoxification, urea and protein synthesis, and P-450-dependent metabolism of p-nitroanisole were maintained for 1.5 days. The bioreactor can be used as a bioartificial liver support apparatus.
Solov'ev
V V
VV
Laboratory of Cytotechnology, Institute of Theoretical and ExperimentalBiophysics, Russian Academy of Sciences, Pushchino. soloval@mail.ru
Akatov
V S
VS
Lezhnev
E I
EI
D000818:Animals; D019149:Bioreactors; D002470:Cell Survival; D002478:Cells, Cultured; D022781:Hepatocytes / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000737:chemistry / Q000378:metabolism*; D019164:Liver, Artificial; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014508:Urea / Q000378:metabolism
D000644:Quaternary Ammonium Compounds; D014508:Urea