A new technique for the analysis of endogenous mediators released following thermal injury.
Few techniques today enable us to measure the complex processes taking place inside a burn wound in vivo. The present in vivo technique was based on a standardised burn model in rat skin. A partial- or full-thickness burn was induced and resulted in a gelatinous oedema located between the skin and the underlying rectus muscle. The oedema has distinct borders to the surrounding connective tissue and is separated and removed easily for further analysis. Myeloperoxidase (MPO) activity used as indicator of neutrofil infiltration was increased significantly in the burn oedema versus non-burned skin. Leukocyte metabolic activity was high as shown by significantly higher free radical formation (ESR) in the oedema than in surrounding burned and non-burned tissue. Leukocyte viability measured by Trypan blue stain was 70% in the oedema of full-thickness burns. In order to decide whether processes taking place in the oedema communicate freely with systemic circulation, we conducted a number of experiments. Results show in burned animals in vivo that intravenous administration of indomethacin induced a strong inhibition of PGE(2) in the burn oedema as compared with saline but, as expected, had no significant effect on LTB(4) synthesis. In conclusion, the present technique allows us to analyse the processes taking place inside the burn wound in vivo and to evaluate the effects of various agents on these processes.
Yregård
L
L
Department of Physiology and Pharmacology, Göteborg University, Göteborg, Sweden.
Löwhagen
P H
PH
Cassuto
J
J
Nilsson
U
U
Lindblom
L
L
Räntfors
J
J
Tarnow
P
P
D000418:Albumins / Q000378:metabolism; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology / Q000627:therapeutic use; D002056:Burns / Q000188:drug therapy / Q000503:physiopathology*; D015232:Dinoprostone / Q000096:biosynthesis; D004195:Disease Models, Animal; D004487:Edema / Q000209:etiology / Q000503:physiopathology*; D005070:Evans Blue; D007213:Indomethacin / Q000494:pharmacology / Q000627:therapeutic use; D007975:Leukotriene B4 / Q000096:biosynthesis; D008297:Male; D009504:Neutrophils / Q000502:physiology; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D000418:Albumins; D000894:Anti-Inflammatory Agents, Non-Steroidal; D007975:Leukotriene B4; D005070:Evans Blue; D009195:Peroxidase; D015232:Dinoprostone; D007213:Indomethacin