Interdomain interactions in oligomeric enzymes: creation of asymmetry in homo-oligomers and role in metabolite channeling between active centers of hetero-oligomers.
Interdomain interactions play an important role in the structural organization of many enzymes and the conformational flexibility of their molecules. In this review, the role of intrasubunit and intersubunit domain-domain interactions in the origins of pre-existent asymmetry of homo-oligomeric D-glyceraldehyde-3-phosphate dehydrogenase and tryptophanyl-tRNA synthetase is discussed on the basis of recent X-ray data and other available information about the properties of these and related enzymes. In addition, a novel key function of interdomain interactions is considered: their potential contribution to intramolecular channeling of intermediates between active centers located on different subunits of a hetero-oligomeric enzyme (alpha,beta-heterodimeric carbamoyl phosphate synthetase).
Nagradova
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A.N. Belozerski Institute of Physico-Chemical Biology, Moscow State University, 119899, Moscow, Russia. serene@cityline.ru
D000818:Animals; D001665:Binding Sites; D002222:Carbamoyl-Phosphate Synthase (Ammonia) / Q000737:chemistry / Q000378:metabolism; D004798:Enzymes / Q000737:chemistry* / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D001411:Geobacillus stearothermophilus / Q000201:enzymology; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000737:chemistry / Q000378:metabolism; D066298:In Vitro Techniques; D008958:Models, Molecular; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D011817:Rabbits; D014369:Tryptophan-tRNA Ligase / Q000737:chemistry / Q000378:metabolism; D014448:Tyrosine-tRNA Ligase / Q000737:chemistry / Q000378:metabolism
D004798:Enzymes; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D014448:Tyrosine-tRNA Ligase; D014369:Tryptophan-tRNA Ligase; D002222:Carbamoyl-Phosphate Synthase (Ammonia)