[Function analysis of regulative region of transforming gene Tx related to human nasopharyngeal carcinoma].
Tx gene is the DNA sequence with transforming activity which was cloned from the epithelial cell line (CNE2) of human nasopharyngeal carcinoma (NPC). In order to understand the role of Tx in carcinogenesis of NPC, the promoter function of potential promoter region in Tx2. 8 was detected using CAT reporter assay. The results showed that the region has no promoter function. It suggested that Tx2. 8 kb fragment homogenous to IGKC might be regulated by the specific mechanism of immunoglobulin gene.
Peng
B
B
Institute of Cancer Research, Hunan Medical University, Changsha 410078.
Zhou
M
M
Cao
L
L
Li
G
G
Yao
K
K
D015500:Chloramphenicol O-Acetyltransferase; D004273:DNA, Neoplasm / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D006801:Humans; D007145:Immunoglobulin kappa-Chains / Q000235:genetics; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology; D009857:Oncogenes / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D016385:TATA Box; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D007145:Immunoglobulin kappa-Chains; D015500:Chloramphenicol O-Acetyltransferase