Intracellular transport of phosphatidic acid and phosphatidylcholine into lipid bodies: use of fluorescent lipids to study lipid-body formation in an oleaginous fungus.
Fluorescent phosphatidic acid and phosphatidylcholine were used to characterize lipid-transport pathways into lipid bodies in an oleaginous fungus, Mortierella ramanniana var. angulispora. Several characteristics of the lipid transport such as temperature dependence and ATP dependence were evaluated. The transport depicted by these fluorescent lipids was consistent with metabolism of radiolabelled lipids, indicating that fluorescent lipids are useful to study lipid-body formation in this fungus. The results dissect lipid transport of phosphatidic acid and phosphatidylcholine into lipid bodies and reveal regulatory steps for lipid-body formation in this fungus.
Kamisaka
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Y
National Institute of Bioscience & Human Technology, Tsukuba, Ibaraki 305-8566, Japan. kamisaka@nibh.go.jp
Noda
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N
D000255:Adenosine Triphosphate / Q000378:metabolism; D001896:Boron Compounds; D002250:Carbon Radioisotopes; D005227:Fatty Acids / Q000378:metabolism; D005456:Fluorescent Dyes; D007700:Kinetics; D019787:Linoleic Acid / Q000378:metabolism; D050356:Lipid Metabolism; D009090:Mucorales / Q000378:metabolism*; D019301:Oleic Acid / Q000378:metabolism; D015388:Organelles / Q000378:metabolism*; D010712:Phosphatidic Acids / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000378:metabolism*; D013816:Thermodynamics
C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D002250:Carbon Radioisotopes; D005227:Fatty Acids; D005456:Fluorescent Dyes; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D019301:Oleic Acid; D000255:Adenosine Triphosphate; D019787:Linoleic Acid