title: Structure-specific abnormalities associated with mutations in a DNA replication accessory factor in Drosophila.
abstract: We have phenotypically and molecularly analyzed the cutlet locus in Drosophila. Homozygous cutlet flies exhibit abnormal development of a subset of adult tissues, including the eye, wing, and ovary. We show that abnormal development of these tissues is due to a defect in normal cell growth. Surprisingly, cell growth is affected in all developing precursor tissues in cutlet mutant animals, including those that give rise to phenotypically wild-type adult structures. The cutlet gene encodes a Drosophila homologue of yeast CHL12 and has similarity to mammalian replication factor C. In addition, cutlet genetically interacts with multiple subunits of Drosophila replication factor C. Our results suggest that the cutlet gene product acts as an accessory factor for DNA replication and has different requirements for the formation of various adult structures during Drosophila development.
authors.affiliation: Cell and Molecular Biology Graduate Group, University of Pennsylvania School of Medicine, Philadelphia 19104, USA.
authors.forename: A B
authors.initials: AB
authors.lastname: Jaffe
authors.forename: T A
authors.initials: TA
authors.lastname: Jongens
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002455:Cell Division; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development*; D005123:Eye / Q000166:cytology; D005260:Female; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics; D018398:Homeodomain Proteins; D006720:Homozygote; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015778:Minor Histocompatibility Antigens; D008938:Mitosis; D008969:Molecular Sequence Data; D009154:Mutation; D010053:Ovary / Q000166:cytology; D010641:Phenotype; D021122:Protein Subunits; D019253:Proto-Oncogene Proteins c-bcl-2; D051818:Replication Protein C; D012097:Repressor Proteins; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014921:Wings, Animal / Q000166:cytology
chemical_list: C096816:BCL2-related protein A1; C092693:CTF18 protein, S cerevisiae; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D005656:Fungal Proteins; D018398:Homeodomain Proteins; D019476:Insect Proteins; C096063:MATA1 protein, S cerevisiae; D015778:Minor Histocompatibility Antigens; D021122:Protein Subunits; D019253:Proto-Oncogene Proteins c-bcl-2; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; C422905:cutlet protein, Drosophila; D051818:Replication Protein C