title: The essential amino acid lysine acts as precursor of glutamate in the mammalian central nervous system.
abstract: Lysine has long been recognized as an essential amino acid for humans and the lack or low supply of this compound in the diet may lead to mental and physical handicaps. Since lysine is severely restricted in cereals, the most important staple food in the world, the understanding of its biological roles must be a major concern. Here we show that lysine is an important precursor for de novo synthesis of glutamate, the most significant excitatory neurotransmitter in the mammalian central nervous system. We also show that the synthesis of glutamate from lysine, which is carried out by the saccharopine pathway, is likely to take place in neurons.
authors.affiliation: Centro de Biologia Molecular e Eugenharia Genetica, Universidade Estadual de Campinas, SP, Brazil.
authors.forename: F
authors.initials: F
authors.lastname: Papes
authors.forename: M J
authors.initials: MJ
authors.lastname: Surpili
authors.forename: F
authors.initials: F
authors.lastname: Langone
authors.forename: J R
authors.initials: JR
authors.lastname: Trigo
authors.forename: P
authors.initials: P
authors.lastname: Arruda
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D002531:Cerebellum / Q000166:cytology / Q000201:enzymology / Q000378:metabolism; D002540:Cerebral Cortex / Q000166:cytology / Q000201:enzymology / Q000378:metabolism; D020869:Gene Expression Profiling; D018698:Glutamic Acid / Q000378:metabolism*; D017403:In Situ Hybridization; D008239:Lysine / Q000378:metabolism*; D051379:Mice; D009474:Neurons / Q000201:enzymology / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D012443:Saccharopine Dehydrogenases / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D018698:Glutamic Acid; D012443:Saccharopine Dehydrogenases; C029496:saccharopine dehydrogenase (NADP+, lysine-forming); D008239:Lysine