Changes in glycogen and trehalose content of Streptomyces brasiliensis hyphae during growth in liquid cultures under sporulating and non-sporulating conditions.
Streptomyces brasiliensis ATCC 23727 showed extensive sporulation when cultured in a liquid medium containing galactose and glutamic acid as carbon and nitrogen sources. Under such conditions, glycogen and trehalose are accumulated in the hyphae coinciding with spore formation. The results reported here suggest that glycogen accumulated in sporogenic hyphae is converted into trehalose during the final period of spore maturation. Glycogen is also accumulated in the hyphae when S. brasiliensis is cultured under conditions which did not support sporulation. Under such conditions, however, glycogen degradation is not accompanied by accumulation of trehalose. This suggest that the conversion of glycogen into trehalose might be a sporulation-specific event in streptomycetes.
Rueda
B
B
Laboratorio de Microbiología, Facultad de Medicina, Universidad de Oviedo, Julián Clavería s/n, 33006, Oviedo, Spain.
Miguélez
E M
EM
Hardisson
C
C
Manzanal
M B
MB
D006003:Glycogen / Q000378:metabolism*; D013171:Spores, Bacterial; D013302:Streptomyces / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D014199:Trehalose / Q000378:metabolism*
D006003:Glycogen; D014199:Trehalose