Mouse embryo toxicity of IL-6 in peritoneal fluids from women with or without endometriosis.
BACKGROUND
To determine whether there is a factor (or factors) in the peritoneal fluid of endometriosis patients that impairs embryo growth and embryo implantation.


METHODS
Growth and development of two-cell mouse embryos which were cultured in media with peritoneal fluid from women with or without endometriosis and interleukin-1-beta (IL-1beta), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) levels in conditioned media were measured.


RESULTS
The blastocyst rate in the non-endometriosis group was 46.4 +/- 31.1%, and that of the endometriosis group was 54.6 +/- 28.7%. Logistic regression analysis using the criteria of blastocyst development in 454 embryos, showed that the peritoneal fluid from endometriosis could promote (p=0.015) but IL-6 could arrest embryo growth to blastocyst (p=0.025). IL-1beta and TNF-alpha levels had no significant effect on blastocyst formation.


CONCLUSIONS
Peritoneal fluid from women with endometriosis was not toxic to mouse embryo development. However, IL-6 in the peritoneal fluid deteriorated the growth and development of mouse embryos.
Wu
M Y
MY
Department of Obstetrics and Gynecology, College of Medicine and the Hospital, National Taiwan University, Taipei.
Chen
S U
SU
Chao
K H
KH
Chen
C D
CD
Yang
Y S
YS
Ho
H N
HN
D000328:Adult; D000818:Animals; D001202:Ascitic Fluid / Q000737:chemistry; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D004715:Endometriosis / Q000150:complications / Q000503:physiopathology*; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000009:adverse effects* / Q000494:pharmacology; D051379:Mice; D008813:Mice, Inbred ICR; D011247:Pregnancy
D015850:Interleukin-6