title: Deamidation of human beta B1 alters the elongated structure of the dimer.
abstract: The purpose of these experiments was to determine if truncation and deamidation alter the structure of a human lens protein, beta B1-crystallin. Recombinant wild type and a deamidated form of recombinant beta B1 were expressed in Escherichia coli. Wild type beta B1 was also enzymatically cleaved to generate a physiologically-relevant truncated beta B1. Purity and size of the expressed proteins were confirmed by SDS-PAGE and electrospray ionization mass spectrometry. Size exclusion chromatography and light scattering were used to determine aggregation states of beta B1. Protein conformations were predicted from sedimentation velocity analysis. Molecular weights of 49,000 and 54,000 Da were obtained for wild type beta B1 by sedimentation equilibrium and light scattering, respectively. A sedimentation coefficient of 2.7 S was determined for wild type beta B1. Molecular weights of 54,000 and 60,000 Da were determined for deamidated beta B1 by sedimentation equilibrium and light scattering, respectively. However, deamidated beta B1 eluted earlier than wild type beta B1 on size exclusion chromatography, with an estimated molecular weight between 78,000 and 116,000 Da. Loss of the extensions of beta B1 caused abnormal association of the protein with the stationary phase during size exclusion chromatography. Wild type beta B1 was predicted to form a dimer with an elongated structure. The earlier elution of the deamidated beta B1 dimer on size exclusion chromatography suggested the dimer was less compact. Truncation caused abnormal column interactions suggesting an altered conformation. These changes are important because truncation and deamidation occur extensively in aging human lenses and may be important for senile cataract formation.
authors.affiliation: Department of Oral Molecular Biology, Oregon Health Sciences University, Portland, OR 97201, USA. lampik@ohsu.edu
authors.forename: K J
authors.initials: KJ
authors.lastname: Lampi
authors.forename: J T
authors.initials: JT
authors.lastname: Oxford
authors.forename: H P
authors.initials: HP
authors.lastname: Bachinger
authors.forename: T R
authors.initials: TR
authors.lastname: Shearer
authors.forename: L L
authors.initials: LL
authors.lastname: David
authors.forename: D M
authors.initials: DM
authors.lastname: Kapfer
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D003459:Crystallins / Q000737:chemistry*; D003641:Deamination; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D006801:Humans; D007223:Infant; D008027:Light; D013058:Mass Spectrometry; D008970:Molecular Weight; D011487:Protein Conformation; D012542:Scattering, Radiation
chemical_list: D003459:Crystallins