[Studies on arachidonic acid production by Mortierella].
The effects of the incubation temperature, initial pH of the medium, carbon source and nitrogen source on the production of arachidonic acid by Mortierella sp. M10 were studied. Thought orthogonal experiments, the optimum culture medium was obtained (g/L): glucose, 100; yeast extract, 10; KNO3, 4.0; KH2PO4, 2.0; CaCl2.2H2O, 0.1; MgSO4.7H2O, 0.5; FeCl3.6H2O, 0.015; ZnSO4.7H2O, 0.0075; CuSO4.5H2O, 0.0005. Under the optimum culture conditions, the dry cell weight and arachidonic acid was 33.51 g/L and 0.827 g/L, respectively. The flask culture process was analysed.
Bao
S
S
Dept. of Bioengineering, South China University of Technology, Guangzhou 510641.
Zhu
F
F
Lin
W
W
Yao
R
R
D016718:Arachidonic Acid / Q000096:biosynthesis*; D003470:Culture Media; D005285:Fermentation; D020094:Mortierella / Q000378:metabolism*
D003470:Culture Media; D016718:Arachidonic Acid