Infrequent occurrence of natural mutations in the pp65(495-503) epitope sequence presented by the HLA A*0201 allele among human cytomegalovirus isolates.
To determine if mutations of an immunodominant HLA-restricted cytomegalovirus (CMV) peptide sequence occur in nature, the sequence corresponding to the HLA A*0201-specific peptide CMVpp65(495-503) was determined in 50 human CMV isolates. Rare mutations were detected; 6 of 50 were silent mutations at the amino terminus of the peptide, while 3 of 50 were mutations of the native methionine residue to isoleucine (M499I). The observed M499I mutation in three isolates decreased cytolytic targeting.
Zaia
J A
JA
Department of Virology, Beckman Research Institute of the City of Hope, Duarte, California 91010, USA. jzaia@coh.org
Gallez-Hawkins
G
G
Li
X
X
Yao
Z Q
ZQ
Lomeli
N
N
Molinder
K
K
La Rosa
C
C
Diamond
D J
DJ
D000483:Alleles; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology; D003587:Cytomegalovirus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000821:virology; D000939:Epitopes / Q000235:genetics*; D015234:HLA-A Antigens / Q000235:genetics* / Q000276:immunology; D006801:Humans; D009154:Mutation; D010750:Phosphoproteins / Q000235:genetics* / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014763:Viral Matrix Proteins / Q000235:genetics* / Q000276:immunology
D000956:Antigens, Viral; D000939:Epitopes; D015234:HLA-A Antigens; D010750:Phosphoproteins; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa