title: Biochemical engineering of natural product biosynthesis pathways.
abstract: Metabolic engineering of natural products is a science that has been built on the goals of traditional strain improvement with the availability of modern molecular biological technologies. In the past 15 years, the state of the art in metabolic engineering of natural products has advanced from the first proof-of-principle experiment based on minimal known genetics to a commonplace event using highly specific and sophisticated gene manipulation methods. With the availability of genes, host organisms, vector systems, and standard molecular biological tools, it is expected that metabolic engineering will be translated into industrial reality.
authors.affiliation: Natural Products Drug Discovery-Microbiology, Merck Research Labs, Rahway, New Jersey 07065, USA. william_strohl@merck.com
authors.forename: W R
authors.initials: WR
authors.lastname: Strohl
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D001688:Biological Products / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D004274:DNA, Recombinant / Q000096:biosynthesis; D005658:Fungi / Q000235:genetics / Q000378:metabolism; D005818:Genetic Engineering / Q000379:methods* / Q000639:trends*; D008969:Molecular Sequence Data; D013379:Substrate Specificity
chemical_list: D001688:Biological Products; D004274:DNA, Recombinant