[Genetic polymorphism of eight STR loci in the Han population in Henan province].
OBJECTIVE
To investigate the allele frequencies of eight short tandem repeats(STR) loci: TH01, FES, D19S400, D7S820, D16S539, D20S161, D3S1545 and D5S818 in Han population in Henan province.


METHODS
DNA was extracted with phenol-chloroform from EDTA-blood samples of the unrelated individuals in Henan province and amplified with PCR technique. The PCR product was analyzed with the undenatured PAGE vertical electrophoresis and silver-stain.


RESULTS
The authors got the frequencies of the eight loci. The heterozygosities of the eight loci are 0.66, 0.67, 0.80, 0.76, 0.79, 0.79, 0.78 and 0.78; the discrimination powers are 0.83, 0.83, 0.94, 0.91, 0.93, 0.93, 0.92 and 0.92.


CONCLUSIONS
The heterozygosities of the eight loci are high and the frequencies are in good agreement with Hardy-Weinberg equilibrium, so the eight loci can be used in individual identification testing.
Hao
B
B
Medical Genetic Institute of Henan Province, the People's Hospital of Henan Province, Zhengzhou, Henan, 450003 P.R. China. mgihnp@public2.zz.ha.cn
Li
Y
Y
Yang
Y
Y
Wang
Y
Y
Huang
F
F
Liao
S
S
Wang
Z
Z
Si
Y
Y
Zhu
W
W
D000483:Alleles; D002681:China / Q000208:ethnology; D002874:Chromosome Mapping; D005787:Gene Frequency; D006579:Heterozygote; D006801:Humans; D011110:Polymorphism, Genetic; D020080:Tandem Repeat Sequences