title: New polymorphisms in the interleukin-10 gene--relationships to myocardial infarction.
abstract: BACKGROUND
  Interleukin-10 (IL-10) is a cytokine with anti-inflammatory and B-cell-stimulating activity. IL-10 is expressed in human atherosclerotic plaques and recent studies have shown the involvement of IL-10 in the atherosclerotic process. Therefore, we hypothesized that polymorphisms in the IL-10 gene might be associated with a predisposition to coronary heart disease.
  
  
  METHODS
  To identify new polymorphisms in the human IL-10 gene, the entire coding sequence and the 3' flanking sequence of the gene were screened by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCR) followed by sequencing. The polymorphisms identified, and three others which have been previously described in the promoter region of the IL-10 gene (G-1082A, C-819T, C-592A), were then investigated in the ECTIM Study, a large population-based case-control study of myocardial infarction.
  
  
  RESULTS
  Four new polymorphisms were identified: one in exon 1 (G+78/ex1A), which predicts a Glycine to Arginine change at position 15 in the putative signal peptide of the protein, two in the intron 3 (C+19/in3T, T+953/in3C) and one in the 3' flanking region (C+117T). All the IL-10 polymorphisms were in complete or nearly complete pairwise linkage disequilibrium. No case-control difference was found in genotype or allele frequencies for any of the polymorphisms.
  
  
  CONCLUSIONS
  Our results suggest that IL-10 polymorphisms are not associated with an increased risk of myocardial infarction.
authors.affiliation: INSERM U525, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Donger
authors.forename: J L
authors.initials: JL
authors.lastname: Georges
authors.forename: V
authors.initials: V
authors.lastname: Nicaud
authors.forename: C
authors.initials: C
authors.lastname: Morrison
authors.forename: A
authors.initials: A
authors.lastname: Evans
authors.forename: F
authors.initials: F
authors.lastname: Kee
authors.forename: D
authors.initials: D
authors.lastname: Arveiler
authors.forename: L
authors.initials: L
authors.lastname: Tiret
authors.forename: F
authors.initials: F
authors.lastname: Cambien
mesh_terms: D000328:Adult; D000368:Aged; D003327:Coronary Disease / Q000453:epidemiology / Q000235:genetics / Q000276:immunology; D017931:DNA Primers; D005091:Exons; D005260:Female; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D007438:Introns; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000453:epidemiology / Q000235:genetics* / Q000276:immunology*; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D012307:Risk Factors
chemical_list: D017931:DNA Primers; D016753:Interleukin-10