Lipoxygenases in early Brassica napus germination.
We have identified transcripts of two lipoxygenase (LOXs) from 3-day-germinated etiolated cotyledons. One encodes a soluble and the other encodes a plastid LOX. They are 2520 bp and 2676 bp in size, respectively. Antibodies raised against the C-terminal 500 amino acids of the plastid LOX recognize a 90 kDa LOX in 5-day-old etiolated and green cotyledons. In mature leaves a LOX of 95 kDa is detected. LOX activity and protein was mainly located in the cytosol.
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Department of Physiology, Carlsberg Laboratory, Gamle Carlsbergvej 10, DK-2500 Valby, Denmark.
Brandt
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D001937:Brassica / Q000201:enzymology* / Q000254:growth & development; D018548:Cotyledon / Q000201:enzymology*; D003624:Darkness; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D006358:Hot Temperature; D007527:Isoenzymes / Q000235:genetics; D008027:Light; D008084:Lipoxygenase / Q000235:genetics*; D018515:Plant Leaves / Q000201:enzymology; D012333:RNA, Messenger / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
D007527:Isoenzymes; D012333:RNA, Messenger; D008084:Lipoxygenase