title: Succinic dehydrogenase histochemistry as an early marker for hair cell pathology.
abstract: Density measurements of succinic dehydrogenase (SDH) activity were obtained from the inner and outer hair cells on surface preparations obtained from the guinea pig cochlea. Guinea pigs were exposed to noise (3.85 kHz, 120 dB SPL, 22.5 min) and sacrificed 0, 4 or 24 h after the exposure. By 4 h after exposure, the first- and second-row outer hair cells already demonstrated an altered SDH activity. By 24 h after exposure, a significant decrease in SDH staining in both the inner and outer hair cells at a distance of 10-12 mm from the cochlear apex was demonstrated. After a 1-month recovery period, scanning electron microscopy confirmed the main lesion site to be at a distance of 10-12 mm. In addition, Hensen's cells (supporting cells) at a distance of 10-12 mm from the apex were intensely stained by SDH after noise exposure, indicating an increase in oxidative metabolism. SDH staining in the Hensen's cells from the unexposed cochleae was not found. In conclusion, our findings suggest that the early use of SDH histochemistry can predict later permanent damage to the organ of Corti.
authors.affiliation: ENT Department, Danderyd Hospital, Danderyd, Sweden.
authors.forename: L
authors.initials: L
authors.lastname: Fredelius
authors.forename: A
authors.initials: A
authors.lastname: Viberg
authors.forename: B
authors.initials: B
authors.lastname: Canlon
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D005260:Female; D006168:Guinea Pigs; D006199:Hair Cells, Auditory, Inner / Q000473:pathology*; D018072:Hair Cells, Auditory, Outer / Q000473:pathology*; D006317:Hearing Loss, Noise-Induced / Q000201:enzymology / Q000473:pathology*; D006651:Histocytochemistry; D008855:Microscopy, Electron, Scanning; D018384:Oxidative Stress; D013385:Succinate Dehydrogenase / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D013385:Succinate Dehydrogenase