title: Cloning and characterization of a cDNA encoding serine palmitoyltransferase in Arabidopsis thaliana.
abstract: The first and committed step in de novo sphingolipid synthesis is catalysed by serine palmitoyltransferase (EC 2.3.1.50), which condenses serine and palmitoyl-CoA to form 3-ketosphinganine in a pyridoxal-5'-phosphate-dependent reaction. We have isolated and characterized a cDNA clone from Arabidopsis thaliana that is homologous to yeast and mammalian LCB2. For a functional identification, the A. thaliana homologous cDNA was expressed in Escherichia coli, which resulted in significant production of new sphinganine in E. coli cells.
authors.affiliation: Department of Biology, Faculty of Science, Konan University, Kobe 658-8501, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Tamura
authors.forename: H
authors.initials: H
authors.lastname: Nishiura
authors.forename: J
authors.initials: J
authors.lastname: Mori
authors.forename: H
authors.initials: H
authors.lastname: Imai
mesh_terms: D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D008322:Mammals; D012333:RNA, Messenger / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D051102:Serine C-Palmitoyltransferase; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D011994:Recombinant Proteins; D000217:Acyltransferases; D051102:Serine C-Palmitoyltransferase; D013110:Sphingosine; C005682:safingol