title: Role of IgE in primary murine Schistosomiasis mansoni.
abstract: Schistosoma mansoni infection proceeds in normal mice in the absence of detectable levels of polyclonal or specific immunoglobulin (Ig)E until worms mature and deposit eggs. Hence, the course of a primary S. mansoni infection is not expected to vary appreciably in mice with defects in the IgE production. Experimental increase of IgE production early after infection may, however, influence worm development. In the first approach towards this goal, BALB/c mice were injected with interleukin(IL)4 to raise the level of endogenously synthesized IgE. A significant increase in serum polyclonal IgE and antischistosome IgG1 during the prepatent period was not associated with significant changes in worm and egg burden or liver pathology. During the second approach, mice were injected with IgE which was affinity purified from serum of BALB/c mice infected for 16 weeks with S. mansoni. The purified IgE bound to carbohydrate-independent epitopes of soluble antigens from 3 h larvae, adult worms and eggs and recognized the schistosomular surface membrane. No differences in worm and egg load or granuloma number and size were noted between untreated and exogenous IgE-injected mice. Together, the data demonstrate that by itself IgE does not influence the outcome of infection in primary murine S. mansoni.
authors.affiliation: Zoology Department, Faculty of Science, Cairo University, Egypt. Rashika@FRCU.EUN.Eg
authors.forename: R
authors.initials: R
authors.lastname: El Ridi
authors.forename: S
authors.initials: S
authors.lastname: Ragab
authors.forename: S
authors.initials: S
authors.lastname: Lewis
authors.forename: A
authors.initials: A
authors.lastname: Afifi
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology; D000947:Antigens, Helminth / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology; D004527:Egg Proteins / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006099:Granuloma / Q000209:etiology / Q000276:immunology; D007116:Immunization, Passive; D017578:Immunoglobulin Class Switching; D007073:Immunoglobulin E / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000494:pharmacology; D012550:Schistosoma mansoni / Q000254:growth & development / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000276:immunology* / Q000473:pathology; D012995:Solubility; D013268:Stimulation, Chemical
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D000954:Antigens, Surface; D004527:Egg Proteins; D000939:Epitopes; D011994:Recombinant Proteins; D015847:Interleukin-4; D007073:Immunoglobulin E