title: CD4+Ki67+ lymphocytes in HIV-infected patients are effector T cells accumulated in the G1 phase of the cell cycle.
abstract: Entry into the cell cycle represents a fundamental step before generating an effector immune response. The relationship between the cell cycle and antigen-driven T cell response remains, however, poorly understood in virus infection, including HIV. We have identified a critical fraction of CD4+CD45RO+ memory T lymphocytes that express the Ki67 antigen in chronically HIV-infected patients. A high accumulation of Ki67 protein is detected in CD4+CD45RO+Ki67+ cells that are in the G1 phase of the cell cycle (92 ¿ 5 %) but not in S and G2 + M phases, in contrast to normal individuals. Of these cells, 20 - 60 % are spontaneously producing IL-2 and IFN-, unlike CD4+CD45RO+ that do not express Ki67. In addition, HIV-p24 antigen is detectable only in a small fraction CD4+Ki67+ cells. In conclusion, CD4+Ki67+ lymphocytes are circulating effector cells accumulated in the G1 phase of the cell cycle and may be involved in the immune surveillance during HIV infection.
authors.affiliation: H¿pital Piti|-Salp|tri¿re, Laboratoire d'Immunologie Cellulaire et Tissulaire Bat. CERVI, Paris, France.
authors.forename: B
authors.initials: B
authors.lastname: Combadère
authors.forename: C
authors.initials: C
authors.lastname: Blanc
authors.forename: T
authors.initials: T
authors.lastname: Li
authors.forename: G
authors.initials: G
authors.lastname: Carcelain
authors.forename: C
authors.initials: C
authors.lastname: Delaugerre
authors.forename: V
authors.initials: V
authors.lastname: Calvez
authors.forename: R
authors.initials: R
authors.lastname: Tubiana
authors.forename: P
authors.initials: P
authors.lastname: Debré
authors.forename: C
authors.initials: C
authors.lastname: Katlama
authors.forename: B
authors.initials: B
authors.lastname: Autran
mesh_terms: D023241:Antiretroviral Therapy, Highly Active; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology*; D005260:Female; D016193:G1 Phase; D015658:HIV Infections / Q000188:drug therapy / Q000276:immunology*; D006801:Humans; D019394:Ki-67 Antigen / Q000032:analysis*; D017493:Leukocyte Common Antigens / Q000032:analysis; D008297:Male
chemical_list: D019394:Ki-67 Antigen; D017493:Leukocyte Common Antigens