Expression of the major heat shock proteins DnaK and GroEL in Streptococcus pyogenes: a comparison to Enterococcus faecalis and Staphylococcus aureus.
One of the outstanding problems in the field of heat shock response has been to elucidate the mechanism underlying the induction of heat shock proteins (HSPs). In this work, we initiate an analysis of the expression of heat shock groEL and dnaK genes and their promoters in S. pyogenes. The synthesis of total cellular proteins was studied upon transfer of a log-phase culture from 37 degrees C to 42 degrees C by performing 5-min pulse-labeling experiments with (35)S-Met. The heat shock responses in the pathogenic Gram-positive cocci, Enterococcus faecalis and Staphylococcus aureus, were also analyzed.
Laport
M S
MS
Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Departamento de Microbiologia Médica, CCS, Bloco I, Cidade Universitária, 21491-590, Rio de Janeiro, RJ, Brazil.
de Castro
A C
AC
Villardo
A
A
Lemos
J A
JA
Bastos
M C
MC
Giambiagi-deMarval
M
M
D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D001483:Base Sequence; D015153:Blotting, Western; D018834:Chaperonin 60 / Q000096:biosynthesis / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D013293:Enterococcus faecalis / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics*; D018869:Heat-Shock Response / Q000235:genetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D013211:Staphylococcus aureus / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D013297:Streptococcus pyogenes / Q000235:genetics* / Q000378:metabolism / Q000502:physiology
D001426:Bacterial Proteins; D018834:Chaperonin 60; D029968:Escherichia coli Proteins; D018840:HSP70 Heat-Shock Proteins; C063842:dnaK protein, E coli