Selectivity in citric acid production by Yarrowia lipolytica.
This experimental study reports about production selectivity in the fermentation of glucose to citric acid by Yarrowia lipolytica as a function of substrate concentration. Batch runs featuring biomass growth and one or two citric acid production phases were carried out in a 15-l stirred tank fermentor. The presented results demonstrate that working at high initial substrate concentration in the production phase is beneficial both in terms of a higher production rate of citric acid, the desired metabolite (reaching 0.077 h(-1)) and of a higher utilization degree of the employed carbon source (yield up to 0.384 g(c.a.)/g(glucose)). The production rate of isocitric acid, the major undesired metabolite, was found to be practically constant over the tested initial substrate concentration range.
Antonucci
S
S
Dipartimento di Ingegneria Chimica, Università di Roma "La Sapienza", Italy
Bravi
M
M
Bubbico
R
R
Di Michele
A
A
Verdone
N
N