Toxoplasma gondii: characterization of temperature-sensitive tachyzoite cell cycle mutants.
We mutagenized RH delta hxgprt strain tachyzoites of Toxoplasma gondii using N-nitroso-N-ethylurea and analyzed 40 clonal isolates (of 3680 ENU mutants) that were unable to grow in cell culture at 40 degrees C. These isolates grew normally at 34 degrees C, but showed variable growth at temperatures between 34 and 39 degrees C. The inability to grow at 40 degrees C was also correlated with a loss of virulence in mice for those mutants examined. We further characterized the temperature-sensitive (ts) isolates using flow cytometry and propidium iodide staining and identified three types of cell cycle-related mutations. Regardless of temperature, in the isolates ts1C12, ts7B4, and ts7B10, the distribution of parasites with a haploid DNA content was substantially higher (congruent with 85%) than that observed for RH delta hxgprt (congruent with 60%). Four other isolates, ts4F6, ts6C11, ts8G10, and ts11F5, contained G1-related mutations, and in each case, the DNA distribution among parasites at the permissive temperature was similar to that of the parental strain, but at 40 degrees C only a single population containing a 1N nuclear DNA complement was evident. Furthermore, there was no evidence of nuclear division or cytokinesis at 40 degrees C, and these parasites demonstrated a distended cytoplasm typical of G1 arrest in other cell types. Finally, parasites of the ts11C9 mutant arrested in two near-equal populations with either 1N or 2N complements of nuclear DNA. All arrested ts11C9 parasites contained a single nucleus, and a major subfraction of the 2N population contained abnormal and incompletely formed daughters-indicating that the initiation of daughter formation can occur in the absence of nuclear division.
Radke
J R
JR
Department of Veterinary Molecular Biology, Montana State University, Bozeman, Montana 59717, USA.
Guerini
M N
MN
White
M W
MW
D000818:Animals; D002453:Cell Cycle / Q000235:genetics*; D002460:Cell Line; D016054:DNA, Protozoan / Q000032:analysis; D005038:Ethylnitrosourea; D005260:Female; D005347:Fibroblasts / Q000469:parasitology; D005434:Flow Cytometry; D006801:Humans; D051379:Mice; D016296:Mutagenesis; D013696:Temperature; D014122:Toxoplasma / Q000166:cytology / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D014124:Toxoplasmosis, Animal / Q000469:parasitology; D014774:Virulence
D016054:DNA, Protozoan; D005038:Ethylnitrosourea