The isochronic band hypothesis and climbing fibre regulation of motricity: an experimental study.
The dynamic organization of the olivocerebellar afferent input to Purkinje cells was examined in rat cerebellar cortex. The distribution of synchronous Purkinje cell complex spike activity was characterized, bilaterally, utilizing multiple electrode recordings in crus IIa folium under ketamine anaesthesia. The results confirmed the existence of rostrocaudal complex spike isochronicity bands with a mediolateral width of 500 microm. For a given band, no finer spatial submicrostructures could be discerned at a first-order approximation (two-dimensional projection). Closer analysis determined that isochronicity between bands is not continuous in space but demonstrates discrete discontinuities at the mediolateral boundaries. Principal component multivariate analysis revealed that the first principal component of the spatio-temporal variance is synchronicity along the rostrocaudal band with a decreased level of coupling in the mediolateral direction at the band boundary. Furthermore, this discrete banding isochronicity is organized by the distribution of feedback inhibition from the cerebellar nuclei on to the inferior olive nucleus. The usual multiple band structure can be dynamically altered to a single wide-band dynamic architecture, or to other patterns of activity, as may be required by movement coordination.
Fukuda
M
M
Department of Behavioural Sciences, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Sugitani, Toyama 930-0194, Japan.
Yamamoto
T
T
Llinás
R
R
D000818:Animals; D002529:Cerebellar Nuclei / Q000166:cytology* / Q000502:physiology*; D004594:Electrophysiology; D005246:Feedback / Q000502:physiology; D005260:Female; D008959:Models, Neurological; D009068:Movement / Q000502:physiology*; D009433:Neural Inhibition / Q000502:physiology; D009434:Neural Pathways / Q000502:physiology; D009847:Olivary Nucleus / Q000166:cytology* / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors