title: Volume-dependent compliance and ventilation-perfusion mismatch in surfactant-depleted isolated rabbit lungs.
abstract: OBJECTIVE
  Volume-dependent alterations of lung compliance are usually studied over a very large volume range. However, the course of compliance within the comparably small tidal volume (intratidal compliance-volume curve) may also provide relevant information about the impact of mechanical ventilation on pulmonary gas exchange. Consequently, we determined the association of the distribution of ventilation and perfusion with the intratidal compliance-volume curve after modification of positive end-expiratory pressure (PEEP).
  
  
  METHODS
  Repeated measurements in randomized order.
  
  
  METHODS
  An animal laboratory.
  
  
  METHODS
  Isolated perfused rabbit lungs (n = 14).
  
  
  METHODS
  Surfactant was removed by bronchoalveolar lavage. The lungs were ventilated thereafter with a constant tidal volume (10 mL/kg body weight). Five levels of PEEP (0-4 cm H2O) were applied in random order for 20 mins each.
  
  
  RESULTS
  The intratidal compliance-volume curve was determined with the slice method for each PEEP level. Concurrently, pulmonary gas exchange was assessed by the multiple inert gas elimination technique. At a PEEP of 0-1 cm H2O, the intratidal compliance-volume curve was formed a bow with downward concavity. At a PEEP of 2 cm H2O, concavity was minimal or compliance was almost constant, whereas higher PEEP levels (3-4 cm H2O) resulted in a decrease of compliance within tidal inflation. Pulmonary gas exchange did not differ between PEEP levels of of 0, 1, and 2 cm H2O. Pulmonary shunt was lowest and perfusion of alveoli with a normal ventilation-perfusion was highest at a PEEP of 3-4 cm H2O. Deadspace ventilation did not change significantly but tended to increase with PEEP.
  
  
  CONCLUSIONS
  An increase of compliance at the very beginning of tidal inflation was associated with impaired pulmonary gas exchange, indicating insufficient alveolar recruitment by the PEEP level. Consequently, the lowest PEEP level preventing alveolar atelectasis could be detected by analyzing the course of compliance within tidal volume without the need for total lung inflation.
authors.affiliation: Department of Anesthesiology and Critical Care Medicine, University of Freiburg, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Mols
authors.forename: G
authors.initials: G
authors.lastname: Hermle
authors.forename: J
authors.initials: J
authors.lastname: Schubert
authors.forename: W
authors.initials: W
authors.lastname: Miekisch
authors.forename: A
authors.initials: A
authors.lastname: Benzing
authors.forename: M
authors.initials: M
authors.lastname: Lichtwarck-Aschoff
authors.forename: K
authors.initials: K
authors.lastname: Geiger
authors.forename: D
authors.initials: D
authors.lastname: Walmrath
authors.forename: J
authors.initials: J
authors.lastname: Guttmann
mesh_terms: D000818:Animals; D018893:Bronchoalveolar Lavage; D004195:Disease Models, Animal; D008170:Lung Compliance; D016555:Matched-Pair Analysis; D011175:Positive-Pressure Respiration / Q000379:methods*; D011659:Pulmonary Gas Exchange; D011817:Rabbits; D011897:Random Allocation; D012128:Respiratory Distress Syndrome / Q000628:therapy*; D015656:Respiratory Mechanics; D018709:Statistics, Nonparametric; D013990:Tidal Volume; D014692:Ventilation-Perfusion Ratio