[A novel in vivo 31P-nuclear magnetic resonance technique for assessment of teratogenicity induced by environmental endocrine disrupters in mice].
Novel in vivo 31P-nuclear magnetic resonance spectoroscopy(NMR) and NMR-imaging techniques for accurate and noninvasive assessment of teratogenicity induced by environmental endocrine disrupters(EDs) were developed. Mice with pregnancy were administered ED at extremely low dose, and then in vivo 31P-NMR spectra of embryos were acquired noninvasively and quantitatively to evaluate the energy metabolism. A significant decrease in embryo ATP level was seen, but no significant changes were detected by conventional histological and biochemical methods. In conclusion, in vivo NMR techniques are highly sensitive(at least 100-fold more sensitive than conventional methods) and are useful for toxicological assessment of environmental pollutants.
Manabe
N
N
Unit of Anatomy and Cell Biology, Department of Animal Sciences, Kyoto University.
Sugimoto
M
M
Morita
M
M
Tanaka
T
T
Yamaguchi
M
M
Miyamoto
H
H
D000014:Abnormalities, Drug-Induced / Q000175:diagnosis* / Q000196:embryology; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D004622:Embryo, Mammalian / Q000378:metabolism*; D004703:Endocrine System / Q000187:drug effects; D004734:Energy Metabolism; D004785:Environmental Pollutants / Q000633:toxicity*; D005260:Female; D006801:Humans; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D011247:Pregnancy; D012680:Sensitivity and Specificity
D004785:Environmental Pollutants; D000255:Adenosine Triphosphate