[Transfer of cry1C gene into Bacillus thuringiensis by electroporation to construct strain with broader insecticidal activity].
Three Transformants were selected by transferring cry1C into Bacillus thuringiensis strain YBT1535. Plasmid profiles, PCR and Southern blot result all proved that cry1C had been transferred into strain YBT1535. Bioassay results showed that the transformants of strain YBT1535 displayed significantly higher toxicity against Spodoptera exigua than strain YBT1535, but the toxicities against Heliothis armigera and Plutella xylostella did not rise except transformant YBT1535-2.
Lu
S Q
SQ
Department of Microbial Science and Technology, Huazhong Agricultural University, Key Laboratory of Agro-microbiology, Ministry of Agriculture, Wuhan 430070.
Liu
Z D
ZD
Yu
Z N
ZN
D001413:Bacillus thuringiensis / Q000235:genetics*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics* / Q000494:pharmacology; D001427:Bacterial Toxins; D018274:Electroporation; D004731:Endotoxins / Q000235:genetics* / Q000494:pharmacology; D018014:Gene Transfer Techniques; D006460:Hemolysin Proteins; D007306:Insecticides / Q000494:pharmacology*
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; C030956:insecticidal crystal protein, Bacillus Thuringiensis