Hydrophobin gene expression affects hyphal wall composition in Schizophyllum commune.
Disruption of the SC3 hydrophobin gene of Schizophyllum commune (DeltaSC3 strain) affected the composition of the cell wall. Compared to a wild-type strain the amount of mucilage (i.e., water-soluble (1-3)beta-glucan with single glucose residues attached by (1-6)beta-linkages) increased considerably, while the amount of alkali-resistant glucan (linked to chitin) decreased. Reintroduction of the SC3 gene or other hydrophobins genes expressed behind the SC3 promotor restored wild-type cell wall composition. However, addition of purified SC3 protein to the medium or growing the DeltaSC3 strain in spent medium of the wild-type strain had no effect. In young cultures of wild-type strains of S.commune, not yet expressing SC3, the amount of mucilage was also relatively high. These data show that hydrophobins not only function at hydrophilic/hydrophobic interfaces, as shown previously, but also affect wall composition.
van Wetter
M A
MA
Molecular Biology of Plants, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Kerklaan 30, Haren, 9715 NN, The Netherlands.
Wösten
H A
HA
Sietsma
J H
JH
Wessels
J G
JG
D002473:Cell Wall / Q000737:chemistry*; D003470:Culture Media; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism*; D012567:Schizophyllum / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
D003470:Culture Media; D005656:Fungal Proteins