Mimotopes for tumor-specific T lymphocytes in human cancer determined with combinatorial peptide libraries.
Mimotopes of a tumor-associated T cell epitope were determined using randomized and combinatorial peptide libraries and a CD8(+) T cell clone specific for the cutaneous T cell lymphoma cell line MyLa. Antigen recognition by this clone was found to be HLA-B8 restricted. More than 80 % of HLA-matched patients with cutaneous T cell lymphoma had mimotope-specific CD8(+) T cells in their peripheral blood. Mimotope-specific T cells isolated and expanded from a patient lysed MyLa cells in in vitro assays thus demonstrating their cytolytic capacity and tumor specificity. Mimotope vaccination of a patient without detectable mimotope-specific T cells induced frequencies of these cells of up to 1.82 % of the peripheral blood CD8(+) T cells.
Linnemann
T
T
Department of Dermatology and Allergy, Charité, Humboldt University, Berlin, Germany. shvdburg@worldonline.nl
Tumenjargal
S
S
Gellrich
S
S
Wiesmüller
K
K
Kaltoft
K
K
Sterry
W
W
Walden
P
P
D000595:Amino Acid Sequence; D000951:Antigens, Neoplasm / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D020650:Combinatorial Chemistry Techniques; D018984:Epitopes, T-Lymphocyte; D015788:HLA-A1 Antigen / Q000235:genetics; D015795:HLA-B8 Antigen / Q000235:genetics; D006801:Humans; D016410:Lymphoma, T-Cell, Cutaneous / Q000276:immunology*; D008969:Molecular Sequence Data
D000951:Antigens, Neoplasm; D018984:Epitopes, T-Lymphocyte; D015788:HLA-A1 Antigen; D015795:HLA-B8 Antigen