Subcellular targeting analysis of SDR-type hydroxysteroid dehydrogenases.
Most mammalian hydroxysteroid dehydrogenases known thus far belong to the protein superfamilies of short-chain dehydrogenases/reductases (SDR) and aldo-keto reductases (AKR). Whereas members of the AKR family are soluble, cytoplasmic enzymes, SDR-type hydroxysteroid dehydrogenases are also located to other subcellular compartments, i.e. endoplasmic reticulum, mitochondria or peroxisomes. Differential localization might play an important role in influencing the reaction direction of hydroxy dehydrogenase/oxo reductase pathways by determining the available nucleotide cofactor pool. Targeting signals for different subcellular organelles in human hydroxysteroid dehydrogenases have been identified, however, in several enzymes localization signals remain to be determined.
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Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 171 77, Stockholm, Sweden.
Wu
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Shafqat
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Hult
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Mårtensson
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Shafqat
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Oppermann
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UC
D042842:11-beta-Hydroxysteroid Dehydrogenases; D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002462:Cell Membrane / Q000201:enzymology; D004721:Endoplasmic Reticulum / Q000201:enzymology; D049452:Green Fluorescent Proteins; D006801:Humans; D006913:Hydroxysteroid Dehydrogenases / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D007527:Isoenzymes / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D008164:Luminescent Proteins / Q000235:genetics; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000201:enzymology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis / Q000737:chemistry; D020675:Peroxisomes / Q000201:enzymology; D011993:Recombinant Fusion Proteins / Q000032:analysis; D016415:Sequence Alignment; D013347:Subcellular Fractions / Q000201:enzymology*; D014162:Transfection
D007527:Isoenzymes; D008164:Luminescent Proteins; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D006913:Hydroxysteroid Dehydrogenases; D042842:11-beta-Hydroxysteroid Dehydrogenases