title: Crystallization of full-length CysB of Klebsiella aerogenes, a LysR-type transcriptional regulator.
abstract: CysB is a positive regulator of transcription of genes involved in cysteine biosynthesis in Gram-negative bacteria and belongs to the large family of LysR-type transcriptional regulators. The full-length protein from Klebsiella aerogenes has been crystallized from solutions containing PEG 8000 in the presence and in the absence of the inducer N-acetylserine by the method of vapour diffusion in hanging drops. For the complexed protein different crystal forms appear in the same drops.
authors.affiliation: York Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, York YO1 5DD, England. koen@ysbl.york.ac.uk
authors.forename: K H
authors.initials: KH
authors.lastname: Verschueren
authors.forename: C
authors.initials: C
authors.lastname: Addy
authors.forename: E J
authors.initials: EJ
authors.lastname: Dodson
authors.forename: A J
authors.initials: AJ
authors.lastname: Wilkinson
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000302:isolation & purification; D019281:Dimerization; D021902:Enterobacter aerogenes / Q000235:genetics; D011092:Polyethylene Glycols; D011487:Protein Conformation; D012694:Serine / Q000031:analogs & derivatives*; D014157:Transcription Factors / Q000737:chemistry; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; C062886:CysB protein, Bacteria; D004268:DNA-Binding Proteins; D014157:Transcription Factors; D011092:Polyethylene Glycols; D012694:Serine; C073470:LysR protein, Bacteria; C012162:N-acetylserine