Genetic analysis of ETS genes in C. elegans.
The recent completion of the Caenorhabditis elegans genome has revealed that this nematode worm has 10 members of the ETS gene family. Isolation and analysis of C. elegans mutants and subsequent screens to identify interacting genes can proceed very quickly in this model organism. Molecular genetic analysis of the receptor tyrosine kinase-Ras-MAP kinase signaling pathway in C. elegans identified the ETS family transcription factor Lin-1 as a nuclear effector of this evolutionarily conserved signal transduction pathway. Here we review classical genetic approaches used to discover the role of Lin-1 in the Ras-MAP kinase signaling pathway and describe new technologies that can be applied to the analyses of signaling pathways and transcription factor regulatory networks in C. elegans.
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Program of Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario, Canada M5G 1X5.
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Bernstein
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D000595:Amino Acid Sequence; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins; D019070:Cell Lineage; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology; D019281:Dimerization; D012734:Disorders of Sex Development / Q000235:genetics; D019143:Evolution, Molecular; D005260:Female; D005544:Forecasting; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D018390:Gene Targeting; D017238:Genes, Helminth; D005821:Genetic Techniques; D015801:Helminth Proteins / Q000235:genetics / Q000502:physiology; D006801:Humans; D007814:Larva; D020935:MAP Kinase Signaling System / Q000235:genetics*; D008297:Male; D051379:Mice; D023421:Models, Animal; D008969:Molecular Sequence Data; D009024:Morphogenesis; D005810:Multigene Family; D016297:Mutagenesis, Site-Directed; D015513:Oncogene Proteins / Q000235:genetics; D010802:Phylogeny; D020543:Proteome; D051789:Proto-Oncogene Protein c-fli-1; D011518:Proto-Oncogene Proteins; D020794:Receptor Protein-Tyrosine Kinases / Q000502:physiology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015534:Trans-Activators / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics* / Q000502:physiology; D000071230:Transcriptional Regulator ERG; D014844:Vulva / Q000166:cytology / Q000254:growth & development; D018631:ras Proteins / Q000502:physiology
D029742:Caenorhabditis elegans Proteins; D004268:DNA-Binding Proteins; C072312:ERG protein, human; C497426:Fli1 protein, mouse; D015801:Helminth Proteins; C097533:Lin-1 protein, C elegans; D015513:Oncogene Proteins; D020543:Proteome; D051789:Proto-Oncogene Protein c-fli-1; D011518:Proto-Oncogene Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D000071230:Transcriptional Regulator ERG; C081464:lin-31 protein, C elegans; D020794:Receptor Protein-Tyrosine Kinases; D018631:ras Proteins