Colorimetric method for the determination of lipoxygenase activity.
A colorimetic assay for lipoxygenase activity has been developed. The assay is based on the detection of the lipoxygenase reaction product, linoleic acid hydroperoxide, by the oxidative coupling of 3-methyl-2-benzothiazolinone (MBTH) with 3-(dimethylamino)benzoic acid (DMAB) in a hemoglobin-catalyzed reaction. This test reaction is rapid and sensitive, and it offers advantages over other methods for detecting lipoxygenase activity. The assay is capable of detecting activity in a number of crude vegetable homogenates and should be particularly useful where a rapid visual determination of lipoxygenase activity is desired.
Anthon
G E
GE
Department of Food Science And Technology, University of California, Davis, California 95616, USA. geanthon@ucdavis.edu
Barrett
D M
DM
D062365:Aminobenzoates / Q000737:chemistry; D052160:Benzothiazoles; D003124:Colorimetry / Q000379:methods*; D006454:Hemoglobins / Q000737:chemistry; D006835:Hydrazones; D007202:Indicators and Reagents; D008041:Linoleic Acids / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D008054:Lipid Peroxides / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D008084:Lipoxygenase / Q000032:analysis* / Q000378:metabolism; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000737:chemistry*; D012996:Solutions; D013844:Thiazoles / Q000737:chemistry; D014675:Vegetables / Q000201:enzymology; D062368:meta-Aminobenzoates
D062365:Aminobenzoates; D052160:Benzothiazoles; D006454:Hemoglobins; D006835:Hydrazones; D007202:Indicators and Reagents; D008041:Linoleic Acids; D008054:Lipid Peroxides; D010936:Plant Extracts; D012996:Solutions; D013844:Thiazoles; D062368:meta-Aminobenzoates; C008285:linoleic acid hydroperoxide; C028079:3-(dimethylamino)benzoic acid; D008084:Lipoxygenase; C002536:3-methyl-2-benzothiazolone hydrazone