title: [Fusion and expression of the gene encoding human Mn-SOD to anti-CEA single-chain antibody in Escherichia coli].
abstract: The gene encoding human manganese-superoxide dismutase (Mn-SOD) was fused to anti-carcinoembryonic antigen single-chain antibody gene to construct the fusion gene, then was ligated into prokaryotic expression vector pET-22b(+), The fusion gene was expressed in E. coli at high level, accounting for 24% of the total bacteria soluble protein; and was characterized by SDS-PAGE and Western-blot analysis; the expression product had the CEA-binding ability in RIA, and also had the SOD activity by pyrogallol autoxidation assay. So, the Mn-SOD moiety retains substantial enzymatic activity, where the ScFv moiety can deliver the fusion protein to tumor, Mn-SOD is a potential tumor-suppressor gene, maybe the fusion protein can provide a new pathway to tumor therapy.
authors.affiliation: Institute of Biochemistry, East China University of Science and Technology, Shanghai 200237.
authors.forename: H J
authors.initials: HJ
authors.lastname: He
authors.forename: W D
authors.initials: WD
authors.lastname: Yang
authors.forename: Y N
authors.initials: YN
authors.lastname: Chang
authors.forename: H J
authors.initials: HJ
authors.lastname: Shi
authors.forename: G Z
authors.initials: GZ
authors.lastname: Yang
authors.forename: X F
authors.initials: XF
authors.lastname: Wu
authors.forename: Q S
authors.initials: QS
authors.lastname: Yuan
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002272:Carcinoembryonic Antigen / Q000276:immunology*; D004926:Escherichia coli / Q000235:genetics*; D006801:Humans; D007128:Immunoglobulin Fragments / Q000235:genetics*; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*; D013482:Superoxide Dismutase / Q000235:genetics*
chemical_list: D002272:Carcinoembryonic Antigen; D007128:Immunoglobulin Fragments; D011993:Recombinant Fusion Proteins; C055966:immunoglobulin Fv; D013482:Superoxide Dismutase