A new subfamily of high molecular mass CDC2-related kinases with PITAI/VRE motifs.
Kinases of the CDC2 family play a key role in cell cycle regulation and gene expression. In the present work, we identified sea urchin and human cDNAs encoding homologues of a high molecular mass CDC2-like kinase (designated CDC2L5) sharing respectively a PITAVRE and PITAIRE motif. The human cDNA encodes the full-length amino acid sequence of the cholinesterase-related cell division controller (CHED) kinase, a previously published partial coding sequence. CDC2L5 overexpressed in mammalian cells is an approximately 170-kDa nuclear protein. The mRNA is present during the sea urchin early embryogenesis and is ubiquitously expressed in human tissues.
Marqués
F
F
Laboratoire Arago, CNRS-UMR 7628/Université Paris VI, BP 44, Banyuls-sur-Mer Cedex, F-66651, France.
Moreau
J L
JL
Peaucellier
G
G
Lozano
J C
JC
Schatt
P
P
Picard
A
A
Callebaut
I
I
Perret
E
E
Genevière
A M
AM
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D042846:CDC2-CDC28 Kinases; D002453:Cell Cycle / Q000502:physiology; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018844:Cyclin-Dependent Kinases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D018076:DNA, Complementary / Q000032:analysis; D004622:Embryo, Mammalian / Q000502:physiology; D004625:Embryo, Nonmammalian; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012617:Sea Urchins / Q000201:enzymology; D017386:Sequence Homology, Amino Acid; D014162:Transfection
D018076:DNA, Complementary; D042846:CDC2-CDC28 Kinases; D018844:Cyclin-Dependent Kinases