Utility of the Arabidopsis FAE1 and yeast SLC1-1 genes for improvements in erucic acid and oil content in rapeseed.
High-erucic acid (HEA) Brassica napus cultivars are regaining interest in industrial contexts. Erucic acid and its derivatives are important renewable raw materials utilized in the manufacture of plastic films, in the synthesis of Nylon 13,13, and in the lubricant and emollient industries. Theoretically, the highest level of erucic acid that can be achieved by means of classical breeding is 66 mol%; however, using new approaches on the basis of genetic engineering, it might be possible to develop a B. napus cultivar containing levels of erucic acid significantly above 66 mol% (>80 mol%). In an attempt to increase the amounts of very-long-chain fatty acids (VLCFAs), and erucic acid in particular, in Canadian HEA B. napus cultivars, we have focused on two targets using a transgenic approach. We examined both the role/function of the Arabidopsis thaliana FAE1 (fatty acid elongase) gene by expressing it under the control of the seed-specific napin promoter in B. napus germplasm with analysis of the changes in VLCFA content in the seed oil of transgenic lines, and the performance of the yeast SLC1-1 (sphingolipid compensation mutant) in B. napus cv. Hero transgenic progeny in the field. Here, we report analyses of the contents of 22:1, total VLCFAand oil in the seed oil, as well as seed yield of the field-grown FAE1 and SLC1-1 B. napus cv. Hero progeny.
Katavic
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Saskatchewan Wheat Pool Agricultural Research and Development, 201-407 Downey Road, Saskatoon, SK, S7N 4L8, Canada. vesna.katavic@nrc.ca
Friesen
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Barton
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Gossen
K K
KK
Giblin
E M
EM
Luciw
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An
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Zou
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MacKenzie
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Keller
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Males
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Taylor
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D000123:Acetyltransferases / Q000235:genetics*; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics*; D001937:Brassica / Q000737:chemistry* / Q000254:growth & development*; D003094:Collagen / Q000235:genetics*; D004883:Erucic Acids / Q000032:analysis*; D000081031:Fatty Acid Elongases; D005227:Fatty Acids / Q000032:analysis*; D005818:Genetic Engineering / Q000379:methods; D010938:Plant Oils / Q000737:chemistry*; D030821:Plants, Genetically Modified / Q000737:chemistry / Q000254:growth & development*; D046988:Proteasome Endopeptidase Complex; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins
D004883:Erucic Acids; D005227:Fatty Acids; D010938:Plant Oils; D029701:Saccharomyces cerevisiae Proteins; C049811:erucic acid; D003094:Collagen; D000123:Acetyltransferases; D000081031:Fatty Acid Elongases; D046988:Proteasome Endopeptidase Complex; C499661:SCL1 protein, S cerevisiae