title: Intracerebroventricular infusion of fibroblast growth factor-1 increases Fos immunoreactivity in periventricular astrocytes in rat hypothalamus.
abstract: When released into the third ventricle of the brain, fibroblast growth factor (FGF)-1 acts as a feeding suppressor. To identify the cells that respond to FGF-1 in this process, we investigated the effects of intracerebroventricular administration of FGF-1 on Fos protein expression in the rat hypothalamus. Two hours after infusion of FGF-1, significantly more Fos signals were observed in the periventricular zone of the third ventricle and its surroundings. Double immunohistochemical studies using antibodies against Fos, glial fibrillary acidic protein, and neuronal nuclei revealed that the FGF-1-induced Fos signals in the periventricular zone were in astrocytes, not in neurons. FGF-1-induced Fos expression was not found in neurons in such hypothalamic nuclei as the lateral hypothalamic area, paraventricular nucleus, ventromedial hypothalamic nucleus, dorsomedial hypothalamic nucleus, or arcuate nucleus. These results suggest a possible involvement of periventricular astrocytes in the early stages of FGF-1-induced feeding suppression.
authors.affiliation: Biosignaling Department, National Institute of Bioscience and Human Technology, AIST, 1-1 Higashi, Ibaraki 305-8566, Tsukuba, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Suzuki
authors.forename: A J
authors.initials: AJ
authors.lastname: Li
authors.forename: T
authors.initials: T
authors.lastname: Akaike
authors.forename: T
authors.initials: T
authors.lastname: Imamura
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D016220:Fibroblast Growth Factor 1; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage / Q000494:pharmacology*; D005456:Fluorescent Dyes; D007150:Immunohistochemistry; D007276:Injections, Intraventricular; D008297:Male; D010286:Paraventricular Hypothalamic Nucleus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005456:Fluorescent Dyes; D016760:Proto-Oncogene Proteins c-fos; D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1