A new approach to the synthesis of APTRA indicators.
We have devised a general synthesis of Mg(2+) indicators which is based on the aminophenol triacetic acid (APTRA) structure. The key step is a palladium-catalyzed coupling reaction of a precursor of the APTRA ligand with a fluorescent group. This strategy resulted in new ratioable fluorescent APTRA indicators and the finding that the fluorescence response of these indicators is different for Mg(2+) and Ca(2+) in some cases. We believe that this represents a generally useful approach for combining fluorophore and chelator functionalities.
Otten
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National Institute of Environmental Health Sciences, Laboratory of Structural Biology, P.O. Box 12233, MR-01, Research Triangle Park, North Carolina 27709, USA.
London
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Levy
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D000085:Acetates / Q000737:chemistry*; D002118:Calcium / Q000737:chemistry; D002384:Catalysis; D002614:Chelating Agents / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis; D007202:Indicators and Reagents; D007502:Iron Chelating Agents / Q000138:chemical synthesis; D008274:Magnesium / Q000737:chemistry*; D010165:Palladium / Q000737:chemistry; D010636:Phenols / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D000085:Acetates; D002614:Chelating Agents; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D007502:Iron Chelating Agents; D010636:Phenols; C424764:aminophenol triacetic acid; D010165:Palladium; D008274:Magnesium; D002118:Calcium