Detection of apolipoprotein A-I, B, and E immunoreactivity in the nuclei of various rat tissue cells.
Proteins with apolipoprotein A-I immunoreactivity were detected in the fraction of non-histone acidic proteins isolated from nuclei of various rat tissue cells. These proteins were detected in the brain, liver, kidney, lung, heart, skeletal muscle, testis, spleen, and bone marrow. In the same fraction from liver nuclei, proteins with apoB and apoE immunoreactivity were also detected. The composition of these proteins was studied by immunoblotting. ApoA-I immunoreactivity in the liver nuclei is due to two proteins. One 28-kD protein corresponds to the mature form of the plasma pool of apoA-I and another 14-kD protein is the product of limited proteolysis of apoA-I. The highest content of apoA-I immunoreactivity was detected in transcriptionally active chromatin and nuclear matrix. ApoB immunoreactivity is due to six proteins with molecular weights from 15 to 100 kD. ApoE immunoreactivity is due to a single protein corresponding to the 35-kD form of plasma apoE. Proteins with apoA-I, apoB, and apoE immunoreactivity may be involved in the regulation of transcriptional activity of chromatin.
Panin
L E
LE
Institute of Biochemistry, Siberian Branch of the Russian Academy of Medical Sciences, Novosibirsk, 630117 Russia. ibch@cyber.ma.nsc.ru
Russkikh
G S
GS
Polyakov
L M
LM
D000818:Animals; D016632:Apolipoprotein A-I / Q000096:biosynthesis*; D001055:Apolipoproteins B / Q000096:biosynthesis*; D001057:Apolipoproteins E / Q000096:biosynthesis*; D002467:Cell Nucleus / Q000378:metabolism*; D002843:Chromatin / Q000378:metabolism; D015151:Immunoblotting; D008099:Liver / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution; D014158:Transcription, Genetic
D016632:Apolipoprotein A-I; D001055:Apolipoproteins B; D001057:Apolipoproteins E; D002843:Chromatin