Survival of Lactobacillus helveticus strain CP53 in the human gastrointestinal tract.
A strain of Lactobacillus helveticus carrying the cryptic plasmid pCP53 was used for a detailed analysis of the survival and persistence of this organism in the faeces of volunteers administered oral doses of the strain. The CP53 strain had high affinity for Caco-2 cells, but displayed low bile acid resistance in vitro. Rifampicin-resistant colonies could be reisolated from the faeces of four of seven subjects fed with the rifampicin-resistant CP53 derivative strain. Moreover, the recovery of cells, as estimated by plasmid analysis, was higher than that estimated by measuring the number of rifampicin-resistant cells. From these results, we conclude that the CP53 strain can survive passage through the gastrointestinal tract.
Shinoda
T
T
R & D Center, Calpis Co. Ltd, Kanagawa, Japan.
Kusuda
D
D
Ishida
Y
Y
Ikeda
N
N
Kaneko
K
K
Masuda
O
O
Yamamoto
N
N
D001647:Bile Acids and Salts / Q000494:pharmacology; D015139:Blotting, Southern; D018938:Caco-2 Cells; D004064:Digestive System / Q000382:microbiology*; D004352:Drug Resistance, Microbial; D005243:Feces / Q000382:microbiology; D006801:Humans; D007778:Lactobacillus / Q000187:drug effects / Q000302:isolation & purification / Q000502:physiology*; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012293:Rifampin / Q000494:pharmacology
D001647:Bile Acids and Salts; D012293:Rifampin