The beauty of the yeast: live cell microscopy at the limits of optical resolution.
The yeast Saccharomyces cerevisiae is a very powerful system for cell biological research. Recent advances in electronic light microscopy together with the application of green fluorescent protein and other in vivo staining techniques have allowed novel and exciting insights into structural organization and dynamics of cells as small as yeast. Methods for staining yeast for microscopic inspection and for introducing tags for localization studies of proteins in living or fixed cells are summarized. Electronic light microscopy, video/deconvolution methods, and confocal laser scanning microscopy as novel tools for structural analyses, and their practical applications in yeast, are discussed.
Kohlwein
S D
SD
SFB Biomembrane Research Center, Department of Biochemistry, Technical University Graz, Petersgasse 12, A-8010 Graz, Austria. kohlwein@ftugax.tu-graz.ac.at
D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D018613:Microscopy, Confocal / Q000379:methods; D018715:Microscopy, Video / Q000379:methods; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure*
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins