title: Denaturing high-performance liquid chromatography (DHPLC) is a highly sensitive, semi-automated method for identifying mutations in the TSC1 gene.
abstract: Sensitive and automated methods for the detection of DNA sequence variation are required for a wide variety of genetic studies. Diagnostic testing in human genetic disorders is one application of such methods. Tuberous sclerosis complex (TSC) is an autosomal dominant familial tumor syndrome characterized by the development of benign tumors (hamartomas) in multiple organs (OMIM # 19110, #191092). There is a high frequency of sporadic cases and significant demand from patients and families for genetic testing information. Two TSC genes have been identified (TSC1 and TSC2) and together account for all cases [1,2]. Here we report our methods for DHPLC analysis of the TSC1 gene and demonstrate the high sensitivity of this method in a blinded analysis of 21 TSC patients with known TSC1 mutations. In this series, DHPLC detected 27/28 (96%) known TSC1 sequence variations. The only sequence variation not identified by DHPLC in this study is a mosaic case.
authors.affiliation: Division of Hematology, Brigham and Women's Hospital, LMRC 301, Boston, MA 02115, USA.
authors.forename: P S
authors.initials: PS
authors.lastname: Roberts
authors.forename: S
authors.initials: S
authors.lastname: Jozwiak
authors.forename: D J
authors.initials: DJ
authors.lastname: Kwiatkowski
authors.forename: S L
authors.initials: SL
authors.lastname: Dabora
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D004252:DNA Mutational Analysis / Q000379:methods* / Q000706:statistics & numerical data; D006579:Heterozygote; D006801:Humans; D009154:Mutation; D009691:Nucleic Acid Denaturation; D011110:Polymorphism, Genetic; D011506:Proteins / Q000235:genetics*; D012680:Sensitivity and Specificity; D014402:Tuberous Sclerosis / Q000235:genetics*; D000077004:Tuberous Sclerosis Complex 1 Protein; D025521:Tumor Suppressor Proteins
chemical_list: D011506:Proteins; C000624650:TSC1 protein, human; D000077004:Tuberous Sclerosis Complex 1 Protein; D025521:Tumor Suppressor Proteins