A simple approach to identify the first rice mutants blocked in carotenoid biosynthesis.
Mutations blocking carotenoid biosynthesis, never before described for rice, are valuable for pathway manipulation and study. Similar to defects in ABA biosynthesis, mutations blocking the carotenoid pathway confer vivipary, but in addition also confer an albino seedling phenotype. Pigments extracted from rice mutants exhibiting the double mutant phenotype were analysed by HPLC (high pressure liquid chromatography); these results led to the identification of the first rice mutant accumulating an intermediate of the carotenoid biosynthetic pathway, phytoene, and a second mutant with almost no detectable carotenoids.
Wurtzel
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Department of Biological Sciences, Lehman College and The Graduate Center, The City University of New York, 250 Bedford Park Blvd. West, Bronx, New York 10468, USA. etwlc@cunyvm.cuny.edu
Luo
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Yatou
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D000040:Abscisic Acid / Q000096:biosynthesis; D002338:Carotenoids / Q000096:biosynthesis*; D002851:Chromatography, High Pressure Liquid; D009154:Mutation; D012275:Oryza / Q000235:genetics* / Q000378:metabolism*; D010641:Phenotype; D010860:Pigments, Biological / Q000235:genetics / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D012639:Seeds / Q000378:metabolism
D010860:Pigments, Biological; D002338:Carotenoids; D000040:Abscisic Acid; C100185:(all-E) phytoene