An RNA polymerase II complex containing capping enzymes and viral oncoproteins.
The aim of this work was to identify proteins from Adenovirus 2-infected HeLa cell extracts that interact with the carboxyl-terminal domain of the largest subunit of RNA polymerase II. First, a mammalian RNA polymerase II complex was isolated from Adenovirus 2-infected HeLa cell extracts by affinity chromatography against the carboxyl-terminal domain of the largest subunit of RNA polymerase II, followed by chromatography on a Mono S fast protein liquid chromatographic column. Second, the isolated complex was further characterized by Western blot analysis, the formation of a GMP-protein complex, and transcriptional activity. The isolated complex contains general transcription factors, chromatin-remodeling factors, histone acetyltransferases, Srbs, capping enzymes, and E1A viral oncoproteins. The RNA polymerase II complex is active in transcription when supplemented with recombinant transcription factor IIB.
Cabrejos
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Programa de Biologia Celular Molecular, Instituto de Ciencias Biomedicas, Facultad de Medicina, Universidad de Chile, Santiago.
Maldonado
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D000123:Acetyltransferases / Q000737:chemistry; D000256:Adenoviridae / Q000378:metabolism*; D017500:Adenovirus E1A Proteins / Q000737:chemistry; D015153:Blotting, Western; D002843:Chromatin / Q000737:chemistry; D002846:Chromatography, Affinity; D002853:Chromatography, Liquid; D006367:HeLa Cells; D051548:Histone Acetyltransferases; D006801:Humans; D015151:Immunoblotting; D009713:Nucleotidyltransferases / Q000378:metabolism; D010455:Peptides / Q000737:chemistry; D012319:RNA Polymerase II / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D012568:Schizosaccharomyces / Q000201:enzymology; D014158:Transcription, Genetic
D017500:Adenovirus E1A Proteins; D002843:Chromatin; D010455:Peptides; D012333:RNA, Messenger; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D000123:Acetyltransferases; D051548:Histone Acetyltransferases; D009713:Nucleotidyltransferases; D012319:RNA Polymerase II; C020842:guanylyltransferase