title: The role of pyridoxal phosphate in the function of EspB, a protein secreted by enteropathogenic Escherichia coli.
abstract: The sequence of EspB, a secreted protein required for virulence of enteropathogenic Escherichia coli (EPEC), reveals a motif common to enzymes that bind pyridoxal phosphate. Pyridoxal phosphate was not found by fluorometry in concentrated supernatants of EPEC cultures that contain EspB. Plasmids containing cloned espB, in which the lysine residue conserved in the motif was substituted with either an arginine or methionine residue, remained capable of complementing an espB deletion mutant to restore EspB function. The results of these studies do not support a role for pyridoxal phosphate in EspB function.
authors.affiliation: Division of Infectious Diseases, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
authors.forename: K A
authors.initials: KA
authors.lastname: Taylor
authors.forename: C B
authors.initials: CB
authors.lastname: O'Connell
authors.forename: R
authors.initials: R
authors.lastname: Thompson
authors.forename: M S
authors.initials: MS
authors.lastname: Donnenberg
mesh_terms: D000199:Actins / Q000032:analysis; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D004926:Escherichia coli / Q000737:chemistry / Q000378:metabolism*; D029968:Escherichia coli Proteins; D005470:Fluorometry; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D011732:Pyridoxal Phosphate / Q000378:metabolism*; D013057:Spectrum Analysis; D013194:Staining and Labeling
chemical_list: D000199:Actins; D001425:Bacterial Outer Membrane Proteins; C087863:EaeB protein, E coli; D029968:Escherichia coli Proteins; D011732:Pyridoxal Phosphate