Identification and typing of Streptomyces strains: evaluation of interspecific, intraspecific and intraclonal differences by RAPD fingerprinting.
The suitability of random amplified polymorphic DNA PCR for the detection of differences between Streptomyces species and strains was evaluated. For this purpose, a protocol of RAPD specific for Streptomyces DNA, i.e. suitable for DNA presenting a high G+C content, was developed using S. ambofaciens ATCC23877. Among the 30 primers tested, all containing 80% G+C, 17 gave a pattern with this strain. Six oligonucleotides were chosen to compare 12 strains belonging to six species of Streptomyces. These oligonucleotides were then used to determine whether these strains could be differentiated at the DNA level with this method. All fingerprints obtained with six primers differed from one species to another. We showed that the RAPD method could be used to reveal intraspecific and intraclonal polymorphisms. Thus, RAPD allows for the rapid, sensitive and specific detection of genetic diversity among species and strains of Streptomyces.
Martin
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P
Laboratoire de génétique et microbiologie, associé à l'institut national de la recherche agronomique, UA 952, Faculté des sciences de l'université Henri Poincaré Nancy 1, Vandoeuvre-lès-Nancy, France.
Dary
A
A
André
A
A
Decaris
B
B
D001482:Base Composition; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D011110:Polymorphism, Genetic / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013302:Streptomyces / Q000145:classification*
D017931:DNA Primers; D004269:DNA, Bacterial