title: Crystallization and preliminary crystallographic study of the periplasmic domain of the Escherichia coli TolR protein.
abstract: The TolR protein from Escherichia coli is part of the Tol-Pal multiprotein complex used by group A colicins to penetrate and kill cells. All genes of the Tol-Pal system are conserved in Gram-negative bacteria and this system is thought to play a role in the maintenance of the bacterial envelope integrity, although its exact function is not known. The TolR protein comprises 142 amino acids. The periplasmic domain of the TolR protein has been expressed, purified and crystallized. The crystals belong to the tetragonal space group P4(1)22, with unit-cell parameters a = 46.3, c = 178.0 A. There are one or two molecules in the asymmetric unit. Frozen crystals diffract to at least 3.2 A resolution using synchrotron radiation. Selenomethionine-substituted periplasmic TolR protein is currently being produced in order to use multiwavelength anomalous dispersion (MAD) for phasing.
authors.affiliation: Information Génétique et Structurale, UMR1889 CNRS-AVENTIS, 31 Chemin Joseph Aiguier, 13402 Marseille, CEDEX 20, France. chantal@igs.cnrs-mrs.fr
authors.forename: C
authors.initials: C
authors.lastname: Abergel
authors.forename: L
authors.initials: L
authors.lastname: Journet
authors.forename: S
authors.initials: S
authors.lastname: Chenivesse
authors.forename: M
authors.initials: M
authors.lastname: Gavioli
authors.forename: R
authors.initials: R
authors.lastname: Lloubès
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry* / Q000302:isolation & purification; D010446:Peptide Fragments / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; D010446:Peptide Fragments; D011994:Recombinant Proteins; C092770:TodR protein, Pseudomonas putida