title: Correlation of CD34+ cell counts with volume of bone marrow collected for allogeneic bone marrow harvests.
abstract: The quantity of bone marrow collected for allogeneic bone marrow transplantation is based on collecting 10 to 15 cc of bone marrow/kg of recipient weight. We hypothesized that the percentage of CD34+ cells collected during a bone marrow harvest decreased at the end of the harvest because of increasing amounts of peripheral blood contamination. We performed a prospective, blinded study in which we measured CD34+ percentages and cell counts at 200-cc intervals during bone marrow harvests from 11 consecutive human leukocyte antigen (HLA)-matched sibling bone marrow donors. We observed that the percentage of CD34+ cells in aspirated bone marrow did not vary significantly from the start to the end of the bone marrow harvest, and the total number of CD34+ cells/kg increased in a linear fashion, thus disproving our original hypothesis. In conclusion, the percentage of CD34+ cells in aspirated bone marrow will remain constant throughout a bone marrow harvest.
authors.affiliation: Wilford Hall Medical Center, Hematology and Oncology/MMIH, Fisher Bone Marrow Transplant Unit, 2200 Bergquist Ste.1, Lackland AFB, TX 78236-5300, USA. pshaughnessy@netscape.net
authors.forename: P J
authors.initials: PJ
authors.lastname: Shaughnessy
authors.forename: B
authors.initials: B
authors.lastname: Kissack
authors.forename: D J
authors.initials: DJ
authors.lastname: Bickford
authors.forename: D W
authors.initials: DW
authors.lastname: Ririe
authors.forename: D L
authors.initials: DL
authors.lastname: Ornstein
authors.forename: J
authors.initials: J
authors.lastname: Lopez
authors.forename: R E
authors.initials: RE
authors.lastname: Hensley
authors.forename: R
authors.initials: R
authors.lastname: Smith
authors.forename: M T
authors.initials: MT
authors.lastname: Schroeder
mesh_terms: D000293:Adolescent; D000328:Adult; D018952:Antigens, CD34 / Q000032:analysis*; D001781:Blood Component Removal / Q000379:methods / Q000592:standards; D001853:Bone Marrow; D016026:Bone Marrow Transplantation / Q000379:methods / Q000592:standards*; D002452:Cell Count; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D006648:Histocompatibility; D006801:Humans; D008297:Male; D008875:Middle Aged; D009679:Nuclear Family; D011446:Prospective Studies; D016037:Single-Blind Method; D014184:Transplantation, Homologous / Q000379:methods / Q000592:standards
chemical_list: D018952:Antigens, CD34