title: Epigenetic change in IGF2R is associated with fetal overgrowth after sheep embryo culture.
abstract: Manipulation or non-physiological embryo culture environments can lead to defective fetal programming in livestock. Our demonstration of reduced fetal methylation and expression of ovine IGF2R suggests pre-implantation embryo procedures may be vulnerable to epigenetic alterations in imprinted genes. This highlights the potential benefits of epigenetic diagnostic screening in developing embryo procedures.
authors.affiliation: Roslin Institute (Edinburgh), Roslin, Midlothian, Scotland, UK. Lorraine.Young@bbsrc.ac.uk
authors.forename: L E
authors.initials: LE
authors.lastname: Young
authors.forename: K
authors.initials: K
authors.lastname: Fernandes
authors.forename: T G
authors.initials: TG
authors.lastname: McEvoy
authors.forename: S C
authors.initials: SC
authors.lastname: Butterwith
authors.forename: C G
authors.initials: CG
authors.lastname: Gutierrez
authors.forename: C
authors.initials: C
authors.lastname: Carolan
authors.forename: P J
authors.initials: PJ
authors.lastname: Broadbent
authors.forename: J J
authors.initials: JJ
authors.lastname: Robinson
authors.forename: I
authors.initials: I
authors.lastname: Wilmut
authors.forename: K D
authors.initials: KD
authors.lastname: Sinclair
mesh_terms: D000015:Abnormalities, Multiple / Q000662:veterinary; D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D019976:Cloning, Organism / Q000662:veterinary*; D000013:Congenital Abnormalities / Q000662:veterinary; D019175:DNA Methylation; D005314:Embryonic and Fetal Development; D005260:Female; D005820:Genetic Testing; D018392:Genomic Imprinting; D011247:Pregnancy; D017527:Receptor, IGF Type 2 / Q000235:genetics*; D012756:Sheep
chemical_list: D017527:Receptor, IGF Type 2