Black tea extract, thearubigin fraction, counteract the effects of botulinum neurotoxins in mice.
Botulinum neurotoxin type A (BoNT/A, 1.5 nM) completely inhibited indirectly evoked twitches in in vitro mouse phrenic nerve-diaphragm preparations within 40 - 45 min. Black tea extract, thearubigin fraction (TRB), mixed with BoNT/A blocked the inhibitory effect of the toxin. The protective effect of TRB extended to botulinum neurotoxins types B and E (BoNT/B and BoNT/E) and tetanus toxin, but not to tetrodotoxin. TRB was also effective against oral toxicity of BoNT/A, B and E. Thus, TRB may be of potential benefit in protecting the paralytic actions of botulinum neurotoxins (BoNTs), but its use is limited by mixing with the toxin.
Satoh
E
E
Department of Pharmacology, University of Obihiro School of Veterinary Medicine, Obihiro 080-8555, Japan.
Ishii
T
T
Shimizu
Y
Y
Sawamura
S
S
Nishimura
M
M
D000818:Animals; D019274:Botulinum Toxins, Type A / Q000633:toxicity*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D009469:Neuromuscular Junction / Q000187:drug effects / Q000502:physiology; D010636:Phenols / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology; D059808:Polyphenols; D013662:Tea
D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D013662:Tea; C086701:thearubigin; D002392:Catechin; D019274:Botulinum Toxins, Type A