title: Antibody-induced activation of beta1 integrin receptors stimulates cAMP-dependent migration of breast cells on laminin-5.
abstract: The beta1 integrin-stimulating antibody TS2/16 induces cAMP-dependent migration of MCF-10A breast cells on the extracellular matrix protein laminin-5. TS2/16 stimulates a rise in intracellular cAMP within 20 min after plating. Pertussis toxin, which inhibits both antibody-induced migration and cAMP accumulation, targets the Galphai3 subunit of heterotrimeric G proteins in these cells, suggesting that Galphai3 may link integrin activation and migration via a cAMP signaling pathway.
authors.affiliation: Department of Biological Sciences, University of Nevada, Las Vegas, Nevada 89154, USA. plopper@nye.nscee.edu
authors.forename: G E
authors.initials: GE
authors.lastname: Plopper
authors.forename: J L
authors.initials: JL
authors.lastname: Huff
authors.forename: W L
authors.initials: WL
authors.lastname: Rust
authors.forename: M A
authors.initials: MA
authors.lastname: Schwartz
authors.forename: V
authors.initials: V
authors.lastname: Quaranta
mesh_terms: D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000473:pathology*; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism / Q000494:pharmacology*; D017931:DNA Primers; D005260:Female; D020962:Heterotrimeric GTP-Binding Proteins / Q000378:metabolism*; D006801:Humans; D039201:Integrin alpha3beta1; D016023:Integrins / Q000378:metabolism*; D037342:Pertussis Toxin; D011233:Precipitin Tests; D017474:Receptors, Laminin / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D014407:Tumor Cells, Cultured; D010566:Virulence Factors, Bordetella / Q000494:pharmacology; D000097794:Kalinin
chemical_list: D000246:Adenosine Diphosphate Ribose; D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D000242:Cyclic AMP; D017931:DNA Primers; D020962:Heterotrimeric GTP-Binding Proteins; D039201:Integrin alpha3beta1; D016023:Integrins; D037342:Pertussis Toxin; D017474:Receptors, Laminin; D010566:Virulence Factors, Bordetella; D000097794:Kalinin