[Evaluation of the diagnostic reagents which detect rotavirus and adenovirus with the immunochromatographical method].
Dipstick 'Eiken' Rota and Dipstick 'Eiken' Adeno were the reagents used for the diagnosis of viral acute gastroenteritis using the immunochromatographical method. Dipstick 'Eiken' Rota and Dipstick 'Eiken' Adeno were evaluated for their sensitivity and specificity. Dipstick 'Eiken' Rota had a capacity to detect a purified rotavirus antigen in a concentration of 50 ng/ml. The sensitivity of Dipstick 'Eiken' Rota was 10 times higher than the sensitivity of the latex agglutination tests (Serodirect 'Eiken' Rota and ROTALEX DRY) and was almost the same as the immunochromatography test (Immu- noCard ST ROTAVIRUS) and the simplified EIA (TESTPACK ROTAVIRUS). With a concentration of 25 ng/ml, Dipstick 'Eiken' Adeno detected a purified adenovirus antigen. Dipstick 'Eiken' Adeno also detected adenovirus antigen in 64 higher dilutions than the latex agglutination test (ADENOLEX DRY). When Dipstick 'Eiken' Rota was compared with Serodirect 'Eiken' Rota among 100 fecal specimens of healthy children and children with viral acute gastroenteritis, the sensitivity was 100.0%, the specificity was 98.0%, and the agreement was 97.0%. When Dipstick 'Eiken' Adeno was compared with ADENOLEX DRY among 55 fecal specimens of healthy children and children with viral acute gastroenteritis, the sensitivity was 100.0  , the specificity was 93.8%, and the agreement was 96.4%. Dipstick 'Eiken' Rota and Dipstick 'Eiken' Adeno are found to be useful diagnostic assays for viral acute gastroenteritis in the clinical laboratories.
Kojima
T
T
Department of Research and Development, Eiken Chemical Co., Ltd., 1-33-8 Hongo, Bunkyo-ku, Tokyo 113-8408, Japan.
Arai
M
M
Sadamoto
S
S
Ikeda
M
M
Araki
K
K
D000256:Adenoviridae / Q000276:immunology / Q000302:isolation & purification*; D000257:Adenoviridae Infections / Q000175:diagnosis; D000956:Antigens, Viral / Q000032:analysis*; D002648:Child; D002845:Chromatography / Q000379:methods*; D005759:Gastroenteritis / Q000175:diagnosis; D006801:Humans; D007124:Immunoenzyme Techniques; D007158:Immunologic Techniques; D007841:Latex Fixation Tests; D012401:Rotavirus / Q000276:immunology / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000175:diagnosis; D012680:Sensitivity and Specificity
D000956:Antigens, Viral