title: Nucleosomes positioned by ORC facilitate the initiation of DNA replication.
abstract: The packaging of eukaryotic DNA into nucleosomes is a critical regulator of nuclear events. To address the interplay between chromatin and replication initiation, we have assessed the determinants and function of the nucleosomal configuration of S. cerevisiae replication origins. Using in vitro and in vivo assays, we demonstrate that the yeast initiator, the origin recognition complex (ORC), is required to maintain the nucleosomal configuration adjacent to origins. Disruption of the ORC-directed nucleosomal arrangement at an origin interferes with initiation of replication, but does not alter the association of ORC with the origin. Instead, the nucleosomes positioned by ORC are important for prereplicative complex formation. These findings suggest that origin-proximal nucleosomes facilitate replication initiation, and that local chromatin structure affects origin function.
authors.affiliation: Department of Biology, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: J R
authors.initials: JR
authors.lastname: Lipford
authors.forename: S P
authors.initials: SP
authors.lastname: Bell
mesh_terms: D002843:Chromatin / Q000235:genetics; D004261:DNA Replication / Q000502:physiology*; D004271:DNA, Fungal / Q000502:physiology; D004268:DNA-Binding Proteins / Q000502:physiology; D005656:Fungal Proteins / Q000502:physiology; D066298:In Vitro Techniques; D009707:Nucleosomes / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D018741:Replication Origin / Q000235:genetics*; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors / Q000502:physiology; D014158:Transcription, Genetic / Q000502:physiology
chemical_list: C061886:ABF1 protein, S cerevisiae; D002843:Chromatin; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D009707:Nucleosomes; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors