Protective effects of (-)-epigallocatechin-3-gallate on UVA- and UVB-induced skin damage.
It has been known that green tea and its components possess significant chemopreventive effects against chemical carcinogens and photo-caused skin tumor formation. In this study, the protective effects of (-)-epigallocatechin-3-gallate (EGCG), a major green tea catechin, on the ultraviolet (UV)-induced skin damage (photoaging) were studied in guinea pigs, hairless mice and human dermal fibroblast cultures. The lipid peroxidation was significantly reduced in the EGCG-treated group. The amount of lipid peroxides produced in the control and EGCG treated group were 838 +/- 144 and 286 +/- 57 nmol/mg at 18 h after UV irradiation, respectively. UVB-induced erythema was also significantly reduced in the EGCG treated group. The erythema relative index of the control and the EGCG treated group were 311 +/- 45 and 191 +/- 49 at 16 h after UV irradiation, respectively. EGCG treatment reduced UVA-induced skin damage (roughness and sagginess) and protected from the decrease of dermal collagen in hairless mouse skin. EGCG treatment blocked the UV-induced increase of collagen secretion and collagenase mRNA level in fibroblast culture. The nuclear transcription factors NF-kappaB and AP-1 binding activities were also inhibited by EGCG treatment.
Kim
J
J
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejon, Korea.
Hwang
J S
JS
Cho
Y K
YK
Han
Y
Y
Jeon
Y J
YJ
Yang
K H
KH
D000818:Animals; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D004586:Electrophoresis; D004890:Erythema / Q000517:prevention & control; D005260:Female; D005347:Fibroblasts; D006168:Guinea Pigs; D007150:Immunohistochemistry; D015227:Lipid Peroxidation / Q000187:drug effects; D051379:Mice; D008812:Mice, Hairless; D016328:NF-kappa B; D012333:RNA, Messenger / Q000096:biosynthesis; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D014466:Ultraviolet Rays
D016328:NF-kappa B; D012333:RNA, Messenger; D011837:Radiation-Protective Agents; D002392:Catechin; D003094:Collagen; C045651:epigallocatechin gallate