title: Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex.
abstract: Accurate segregation of sister chromatids during mitosis is necessary to avoid the aneuploidy found in many cancers. The spindle checkpoint, which monitors the metaphase to anaphase transition, has been shown to be defective in cancers with chromosomal instability. This checkpoint regulates the anaphase-promoting complex or cyclosome (APC/C), a cell cycle ubiquitin ligase regulating among other things sister chromatid separation. We have previously investigated the mouse Apc1 protein (previously also called Tsg24), the largest subunit of the APC/C. We have now sequenced a full-length human APC1 cDNA, mapped its chromosomal location, and analysed its intron-exon boundaries. We have also investigated the RNA and protein expression of the Apc1 and other APC/C components in normal and cancer cells and the relative occurrence of expressed sequence tags (ESTs) representing APC subunits from different tissues. The different APC/C subunits are expressed in most tissues and cell types at fairly constant levels relative to each other, suggesting that they perform their functions as part of a complex. A difference from this pattern is however seen for the APC6, which in some cases is more strongly expressed, suggesting a special function for this protein in certain tissues and cell types.
authors.affiliation: Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: P M
authors.initials: PM
authors.lastname: Jörgensen
authors.forename: S
authors.initials: S
authors.lastname: Gräslund
authors.forename: R
authors.initials: R
authors.lastname: Betz
authors.forename: S
authors.initials: S
authors.lastname: Ståhl
authors.forename: C
authors.initials: C
authors.lastname: Larsson
authors.forename: C
authors.initials: C
authors.lastname: Höög
mesh_terms: D000595:Amino Acid Sequence; D064173:Anaphase-Promoting Complex-Cyclosome; D064189:Apc1 Subunit, Anaphase-Promoting Complex-Cyclosome; D001483:Base Sequence; D002889:Chromosomes, Human, Pair 2; D003001:Cloning, Molecular; D005091:Exons; D020224:Expressed Sequence Tags; D005786:Gene Expression Regulation; D006801:Humans; D007438:Introns; D008025:Ligases / Q000235:genetics*; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics*; D017421:Sequence Analysis; D014407:Tumor Cells, Cultured; D043743:Ubiquitin-Protein Ligase Complexes; D044767:Ubiquitin-Protein Ligases
chemical_list: C577335:ANAPC1 protein, human; C577336:Anapc1 protein, mouse; D064189:Apc1 Subunit, Anaphase-Promoting Complex-Cyclosome; D011506:Proteins; D043743:Ubiquitin-Protein Ligase Complexes; D064173:Anaphase-Promoting Complex-Cyclosome; D044767:Ubiquitin-Protein Ligases; D008025:Ligases