title: Atomic resolution structure of a mutant of the spectrin SH3 domain.
abstract: The crystal structure of an alpha-spectrin Src-homology 3 (SH3) domain mutant has been refined at 1.12 A resolution. This X-ray structure is at the highest resolution achieved so far for an SH3 domain. The structure allows the identification of a complete set of specific interactions and is useful for the elucidation of relations between structure and pH-dependent thermodynamic stability in a series of SH3 domain mutants.
authors.affiliation: Centro di Studio di Biocristallografia, CNR and Dipartimento di Chimica, Università di Napoli Federico II, Via Mezzocannone 4, I-80134 Napoli, Italy. rberisio@chemistry.unina.it
authors.forename: R
authors.initials: R
authors.lastname: Berisio
authors.forename: A
authors.initials: A
authors.lastname: Viguera
authors.forename: L
authors.initials: L
authors.lastname: Serrano
authors.forename: M
authors.initials: M
authors.lastname: Wilmanns
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D006860:Hydrogen Bonding; D008958:Models, Molecular; D009154:Mutation; D017433:Protein Structure, Secondary; D012680:Sensitivity and Specificity; D013049:Spectrin / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D018909:src Homology Domains
chemical_list: D013049:Spectrin