[Monomorphic species specific DNA, detected by polymerase chain reaction with random primers].
Intra- and interspecific variability of total DNA isolated from haploid megagametophytes of coniferous species was examined using polymerase chain reaction with random primers. Based on this technique, one can with certainty detect heterozygosity at gene loci carrying null alleles and thus reveal cryptic intraspecific genetic variation. Large population samples were used. Along with random amplified polymorphic DNA, i.e., widely known fragments (amplicons) polymorphic within a species, we found invariant loci lacking individual or geographic variability but differentiating species within genera and other taxa. This DNA was termed RAMD (random amplified monomorphic DNA) to distinguish it from polymorphic DNA. Our findings suggest that genetic monomorphism of species and the dual structure of the eukaryotic genome can be detected at the DNA level as was previously shown for protein gene markers.
Altukhov
Iu P
IuP
Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 117809 Russia. yualt@vigg.ru
Abramova
A B
AB
D017931:DNA Primers; D018744:DNA, Plant / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006579:Heterozygote; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D013045:Species Specificity
D017931:DNA Primers; D018744:DNA, Plant