Enzymic activities and gene expression of enzymes of the acyl-CoA elongase during rapeseed development.
Enzymic activities and gene expression of oleoyl-CoA elongase were studied during seed development using two different rapeseed cultivars, high-erucic-acid rapeseed (HEAR) and low-erucic-acid rapeseed (LEAR). The overall elongase activities were maximal in HEAR between the fourth and eighth weeks after pollination (WAP) and absent in LEAR. The 3-ketoacyl-CoA synthase (condensing enzyme, CE) mRNA levels and the developmental profiles in the two cultivars were different since maximal expression levels were detected in HEAR and LEAR at WAP 4 and WAP 6, respectively. Anti-CE antibodies revealed two proteins of 60 and 67 kDa in both cultivars and an additional reacting protein of 57 kDa in HEAR.
Puyaubert
J
J
Laboratoire de Biogenèse membranaire UMR 5544 CNRS Université V. Segalen Bordeaux 2, 146 rue Léo Saignat 33076 Bordeaux Cedex, France.
Garbay
B
B
Dieryck
W
W
Costaglioli
P
P
Roscoe
T
T
Cassagne
C
C
Lessire
R
R
D051080:Acyl-Carrier Protein S-Malonyltransferase; D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism*; D001937:Brassica / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012639:Seeds / Q000201:enzymology
D000217:Acyltransferases; D051080:Acyl-Carrier Protein S-Malonyltransferase