Identification of two proteins, S14 and UIP1, that interact with UCH37.
By the use of the yeast two-hybrid screen we have identified two proteins that interacted with UCH37: S14, which is a subunit of PA700 and a novel protein, UIP1 (UCH37 interacting protein 1). The interaction of UCH37 with S14 or UIP1 was confirmed by in vitro binding assay and in vivo co-immunoprecipitation analysis. The C-terminal extension of UCH37 is essential for interaction with S14 or UIP1 as shown by the yeast two-hybrid assay and the in vitro binding assay. Furthermore, UIP1 blocked the interaction between UCH37 and S14 in vitro.
Li
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T
Department of Biochemistry, Faculty of Medicine, National University of Singapore, 10 Kent Ridge Crescent, 119260, Singapore, Singapore.
Duan
W
W
Yang
H
H
Lee
M K
MK
Bte Mustafa
F
F
Lee
B H
BH
Teo
T S
TS
D000595:Amino Acid Sequence; D001665:Binding Sites; D001667:Binding, Competitive; D015153:Blotting, Western; D002268:Carboxypeptidases; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D006801:Humans; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D046988:Proteasome Endopeptidase Complex; D011485:Protein Binding; D021122:Protein Subunits; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D016415:Sequence Alignment; D013869:Thiolester Hydrolases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D043222:Ubiquitin Thiolesterase
D002352:Carrier Proteins; C085834:PA700 proteasome activator; D021122:Protein Subunits; D011506:Proteins; D012333:RNA, Messenger; D013869:Thiolester Hydrolases; D002268:Carboxypeptidases; C421245:UCHL5 protein, human; D043222:Ubiquitin Thiolesterase; D046988:Proteasome Endopeptidase Complex