Distribution of 17beta-hydroxysteroid dehydrogenases in human osteoblast-like cells.
In bone androgens and estrogens exert profound osteoprotective effects. Cultured human osteoblast (hOB)-like cells are able to metabolize circulating androgens or androgen precursors, such as testosterone and androstenedione, respectively, by aromatization (aromatase), 5alpha-reduction (5alpha-reductase) and reduction/oxidation at the 17beta-position (17-beta-hydroxysteroid dehydrogenases, 17beta-HSDs). In this study it was demonstrated that cultured normal human osteoblast-like cells as well as the osteosarcoma cell lines HOS and MG 63 express 17beta-HSDs types 1, 2, 3 and 4.
Feix
M
M
Endocrinology, Department of Internal Medicine, University of Bonn, Wilhelmstr. 35-37, D53111, Bonn, Germany. m.feix@uni-bonn.de
Wolf
L
L
Schweikert
H U
HU
D015067:17-Hydroxysteroid Dehydrogenases / Q000032:analysis* / Q000378:metabolism; D000735:Androstenedione / Q000378:metabolism; D002478:Cells, Cultured; D006801:Humans; D007527:Isoenzymes / Q000032:analysis / Q000378:metabolism; D010006:Osteoblasts / Q000201:enzymology*; D012516:Osteosarcoma / Q000201:enzymology; D016133:Polymerase Chain Reaction; D013739:Testosterone / Q000378:metabolism; D014407:Tumor Cells, Cultured
D007527:Isoenzymes; D013739:Testosterone; D000735:Androstenedione; D015067:17-Hydroxysteroid Dehydrogenases