AP-PCR assay of DNA alterations in the progeny of male mice exposed to low-level gamma-radiation.
By comparative analysis of fingerprints of arbitrarily primed polymerase chain reaction (AP-PCR) products, DNA alterations in somatic cells of the progeny (F1 generation) of male mice chronically exposed to low-doses of gamma-radiation was investigated. Male BALB/c mice exposed to 10-50 cGy were mated with unirradiated females 15 days after irradiation. DNA was isolated from biopsies taken from tail tips of 2-month-old progeny. Preliminary AP-PCRs were carried out with 17 primers representing core sequences of micro- and/or minisatellites or their flanking oligonucleotides. Best quantitatively reproduced AP-PCR fingerprints of genomic DNA were obtained with one of these primers, a 20-mer oligonucleotide flanking the micro-satellite locus Atplb2 on mouse chromosome 11. Comparative analysis of individual fingerprints of AP-PCR products obtained on DNA templates from the progeny of irradiated and intact males revealed an increased variability of micro-satellite-associated sequences and an increased frequency of "non-parental bands" in DNA-fingerprints from the progeny of males chronically exposed to gamma-radiation 15 days before mating (at the postmeiotic stage of spermatogenesis). The results show that increased micro-satellite instability can be initiated by irradiation of the male parent to subsequently arise or be transmitted to the soma of the F1 generations.
Vasil'eva
G V
GV
Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences, Pushchino, 142290, Moscow, Russia.
Bezlepkin
V G
VG
Lomaeva
M G
MG
Sirota
N P
NP
Gaziev
A I
AI
D000818:Animals; D002875:Chromosomes / Q000235:genetics; D016172:DNA Fingerprinting / Q000379:methods*; D004252:DNA Mutational Analysis / Q000379:methods*; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005720:Gamma Rays / Q000009:adverse effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018895:Microsatellite Repeats / Q000235:genetics / Q000528:radiation effects*; D009154:Mutation; D018812:Paternal Exposure; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results