The ameliorating effect of the water layer of Fructus Schisandrae on cycloheximide-induced amnesia in rats: interaction with drugs acting at neurotransmitter receptors.
Our previous study indicated that the water layer present in Fructus Schisandra(FS(w)) at 10 and 25 mg kg(-1)significantly counteracted cycloheximide (CXM)-induced amnesia. Therefore, the mechanism of action of the ameliorating effect of FS(w)on CXM-induced amnesia in the passive avoidance task was investigated in rats. The ameliorating effect of FS(w)on CXM-induced amnesia was depressed by scopolamine. The serotonin releaser, p -chloroamphetamine significantly antagonized the ameliorating effect of FS(w)on CXM-induced amnesia. Furthermore, the ameliorating effect was also inhibited by the 5-HT(1A)receptor agonist 8-OH-DPAT, but potentiated by the 5-HT(2)receptor antagonist ritanserin. Finally, the GABA(A)receptor antagonist bicuculline blocked the ameliorating effect of FS(w). These results suggest that the beneficial effect of FS(w)on CXM-induced amnesia is amplified by treatment with serotonergic 5-HT(2)receptor antagonists, but reduced by serotonergic 5-HT(1A)receptor agonists as well as GABA(A)and cholinergic receptor antagonists.
Hsieh
M T
MT
Institute of Chinese Pharmaceutical Sciences, China Medical College, 91 Hsueh Shih Road, Taichung, Taiwan, 40421, Republic of China. crw@mail.cmc.edu.tw
Wu
C R
CR
Wang
W H
WH
Lin
L W
LW
D000284:Administration, Oral; D000647:Amnesia / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D000818:Animals; D001640:Bicuculline / Q000008:administration & dosage / Q000378:metabolism; D003513:Cycloheximide / Q000494:pharmacology*; D034242:Cyclooctanes; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D017705:Lignans / Q000627:therapeutic use*; D008297:Male; D008517:Phytotherapy; D010946:Plants, Medicinal / Q000627:therapeutic use*; D011083:Polycyclic Compounds / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011930:Reaction Time / Q000187:drug effects; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D016713:Ritanserin / Q000008:administration & dosage / Q000378:metabolism; D012601:Scopolamine / Q000008:administration & dosage / Q000378:metabolism; D014867:Water / Q000737:chemistry; D010133:p-Chloroamphetamine / Q000008:administration & dosage / Q000378:metabolism
D034242:Cyclooctanes; D004365:Drugs, Chinese Herbal; D017705:Lignans; D011083:Polycyclic Compounds; D017981:Receptors, Neurotransmitter; D014867:Water; D016713:Ritanserin; D010133:p-Chloroamphetamine; D003513:Cycloheximide; D012601:Scopolamine; C011105:schizandrin; D001640:Bicuculline