Immunodetection by quartz crystal microbalance. A new approach for direct detection of rabbit IgG and peroxidase.
Biodetection is one of the most important challenges for the twenty-first century: many fields are concerned, mainly environmental and medical. The quartz crystal microbalance (QCM) may offer great possibilities for this purpose: a direct response signal, which characterizes the binding event between a sensitive layer, immobilized onto the surface transducer, and the analyte to be detected, can be obtained. However, for the detection of small biomolecules such as antigens, it is quite difficult to obtain an observable signal that corresponds directly to the binding event. In general, this is owing to the lack of mass sensitivity of the commonly used QCM, with 5- to 10-MHz quartz crystals. For improving this mass sensitivity, a 27-MHz quartz resonator was developed and incorporated in a flow-through microcell. Two biospecies, IgG rabbit and peroxidase enzyme, were studied with this ultra-sensitive QCM in terms of specificity, detection limit, and calibration curve.
Bizet
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K
UPR15 du CNRS, Physique des Liquides et Electrochimie, Université Pierre et Marie Curie, Tour 22, 4, Place Jussieu, 75252 Paris, France. perrot@ccr.jussieu.fr
Gabrielli
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C
Perrot
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H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation*; D002138:Calibration; D007074:Immunoglobulin G / Q000032:analysis* / Q000737:chemistry*; D051379:Mice; D015233:Models, Statistical; D009195:Peroxidase / Q000032:analysis* / Q000737:chemistry*; D011791:Quartz; D011817:Rabbits; D012680:Sensitivity and Specificity; D013997:Time Factors
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D011791:Quartz; D009195:Peroxidase