Surface plasmon resonance based pesticide assay on a renewable biosensing surface using the reversible concanavalin A monosaccharide interaction.
A competitive immunoassay based on surface plasmon resonance (SPR) for the detection of the pesticide 2,4-dichlorophenoxyacetic acid (2,4-D) is reported. The novelty of the assay is based on the regeneration of the chip surface by the reversible interaction between monosaccharide (D-glucose) and lectin (Concanavalin A). Concanavalin A-2,4-D conjugate was chemically synthesized, purified and used for binding to the SPR chip modified with covalently bound alpha-D-glucose. The interaction between anti-2,4-D antibody and the surface-bound concanavalin A-2,4-D conjugate was monitored by surface plasmon resonance and the response was used for the quantification of 2,4-D. The dynamic range of the calibration curve was between 3 and 100 ng/ml. The demonstrated principle of surface regeneration based on the reversible sugar-lectin interaction may be of more general applicability in immunoassays.
Svitel
J
J
Department of Pure and Applied Biochemistry, Center for Chemistry and Chemical Engineering, Lund University, Sweden.
Dzgoev
A
A
Ramanathan
K
K
Danielsson
B
B
D015084:2,4-Dichlorophenoxyacetic Acid / Q000032:analysis*; D015374:Biosensing Techniques; D003208:Concanavalin A / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D006540:Herbicides / Q000032:analysis*; D007118:Immunoassay; D020349:Surface Plasmon Resonance
D006540:Herbicides; D003208:Concanavalin A; D015084:2,4-Dichlorophenoxyacetic Acid; D005947:Glucose