title: Estimation of microsatellite mutation rates in Drosophila melanogaster.
abstract: Microsatellite mutations were studied in a set of 175 mutation accumulation lines, all of them independently derived from a completely homozygous population of Drosophila melanogaster and maintained under strong inbreeding during 80 generations. We assayed 28 microsatellites and detected two mutations. One mutation consisted of a single addition of a dinucleotide repeat and the other was a deletion of five trinucleotide repeats. The average mutation rate was 5.1 x 10(-6), in full agreement with previous estimates from two different sets of mutation accumulation lines.
authors.affiliation: Area de Genética, Departamento de Biología Funcional, Universidad de Oviedo, 33071 Oviedo, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Fernando Vázquez
authors.forename: T
authors.initials: T
authors.lastname: Pérez
authors.forename: J
authors.initials: J
authors.lastname: Albornoz
authors.forename: A
authors.initials: A
authors.lastname: Domínguez
mesh_terms: D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000032:analysis; D004331:Drosophila melanogaster / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D009154:Mutation / Q000235:genetics*; D010957:Plasmids; D016133:Polymerase Chain Reaction
chemical_list: D004247:DNA