Enzymatic preparation of genuine prosapogenin, 20(S)-ginsenoside Rh1, from ginsenosides Re and Rg1.
It was found that a lactase preparation from Penicillium sp. nearly quantitatively hydrolyzed ginsenosides Re and Rg1, which are major saponins in roots of Panax ginseng, to a minor saponin, 20(S)-ginsenoside Rh1 [6-O-beta-D-glucopyranosyl-20(S)-protopanaxatriol]. This is the first report on the enzymatic preparation of ginsenoside Rh1 with a high efficiency. This enzyme also readily hydrolyzed ginsenoside Rg2 to ginsenoside Rh1.
Ko
S R
SR
Korea Ginseng and Tobacco Research Institute, Taejon.
Suzuki
Y
Y
Choi
K J
KJ
Kim
Y H
YH
D001671:Biochemistry / Q000379:methods; D036145:Ginsenosides; D006868:Hydrolysis; D043322:Lactase; D012503:Saponins / Q000138:chemical synthesis* / Q000737:chemistry* / Q000378:metabolism; D001616:beta-Galactosidase / Q000737:chemistry* / Q000378:metabolism
D036145:Ginsenosides; D012503:Saponins; C049864:ginsenoside Re; C026474:ginsenoside Rg2; C425564:ginsenoside Rh1; D043322:Lactase; D001616:beta-Galactosidase; C035054:ginsenoside Rg1