Factors affecting the production of (-)-mitorubrinic acid by Penicillium funiculosum.
The optimum conditions for the production of (-)-mitorubrinic acid by mutant strain of Penicillium funiculosum CCM F-8080 were examined in shaken flask cultures. The highest production of this metabolite was achieved in a medium with glucose as the sole carbon source and (NH4)2HPO4 as the nitrogen source. The most suitable concentrations of the given sources for production of (-)-mitorubrinic acid are 80 g/l glucose and 2 g/l (NH4)2HPO4 (maximal production, 814.9 mg/l). We also observed the influence of concentration of L-phenylalanine and inorganic phosphate in culture medium on the production of (-)-mitorubrinic acid. The biological activity of mitorubrinic acid as trypsin inhibitor was determined (IC50 41.05 mumol/l).
Lesová
K
K
Department of Biochemical Technology, Faculty of Chemical Technology, Slovak University of Technology, Radlinského 9, 81237 Bratislava, Slovakia. lesova@chelin.chtf.stuba.sk
Sturdíková
M
M
Rosenberg
M
M
D001565:Benzoates / Q000138:chemical synthesis*; D002244:Carbon / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D009584:Nitrogen / Q000378:metabolism; D010407:Penicillium / Q000254:growth & development / Q000378:metabolism*; D010649:Phenylalanine / Q000378:metabolism
D001565:Benzoates; D003470:Culture Media; D010649:Phenylalanine; C433878:mitorubrinic acid; D002244:Carbon; D009584:Nitrogen