In situ derivatisation using pressurized liquid extraction to determine phenols, sterols and carboxylic acids in environmental samples and microbial biomasses.
Pressurized liquid extraction was combined with in-situ derivatisation to extract polar analytes such as phenols (including chlorophenols) sterols and carboxylic acids from environmental and microbial samples. This one-step protocol uses acetic anhydride as an acetylation agent, N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) as an silylation agent, and boron trifluoride-methanol, phenyltrimethyl ammoniumhydroxide and trimethyl sulfoniumhydroxide as methylation agents. It results in faster extraction rates and better or comparable extraction efficiencies when compared to classical approaches. The addition of a silylation agent also facilitates the extraction kinetics of analytes not accessible to silylation (e.g. polycyclic aromatic hydrocarbons or alkylbenzenes). This may be attributed to a dissociative action of the agent to weaken analyte-matrix interactions.
Pörschman
J
J
Centre for Environmental Research Leipzig-Halle (UFZ), Department of Remediation Research, Germany. poerschm@san.ufz.de
Plugge
J
J
Toth
R
R
D000107:Acetylation; D018533:Biomass; D002264:Carboxylic Acids / Q000032:analysis*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D004785:Environmental Pollutants / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D008745:Methylation; D010636:Phenols / Q000032:analysis*; D011312:Pressure; D012821:Silanes / Q000737:chemistry; D012987:Soil / Q000032:analysis; D013261:Sterols / Q000032:analysis*
D002264:Carboxylic Acids; D004785:Environmental Pollutants; D010636:Phenols; D012821:Silanes; D012987:Soil; D013261:Sterols