A fluorescent-labeled oligopeptide for monitoring PKA-mediated phosphorylation.
A fluorescent-labeled oligopeptide (DACM-CLRRASLK-fluorescein), containing a consensus amino acid sequence (RRXSL) of cyclic AMP (cAMP) dependent protein kinase A (PKA) substrate-proteins, was designed. The fluorescent peptide was a good substrate of PKA, and the phosphorylation of its serin residue caused an intensive change in fluorescent intensity. We expect that the peptide will be useful as a fluorescent indicator for monitoring PKA activity in living cells.
Ohuchi
Y
Y
Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
Katayama
Y
Y
Maeda
M
M
D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000302:isolation & purification*; D009842:Oligopeptides / Q000302:isolation & purification*; D010766:Phosphorylation; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D009842:Oligopeptides; D017868:Cyclic AMP-Dependent Protein Kinases