title: Icodextrin effluent leads to a greater proliferation than glucose effluent of human mesothelial cells studied ex vivo.
abstract: OBJECTIVE
  To compare the effect of glucose (Glu) and icodextrin (Ico) dialysate on in vitro culture of mesothelial cells (MC) from peritoneal dialysis (PD) patients.
  
  
  METHODS
  Prospective, controlled comparative study on the effects of two PD solutions.
  
  
  METHODS
  A tertiary-care public university hospital.
  
  
  METHODS
  Sixteen PD patients regularly using Glu dialysate were asked to collect an 8-hour dwell peritoneal effluent on 2 different days, with an interval shorter than 7 days. In the first collection, 2.27% Glu solution and in the last, 7.5% Ico solution was infused. Human MC were isolated from the nocturnal peritoneal effluent bags and grown ex vivo.
  
  
  METHODS
  Mesothelial cell proliferative capacity ex vivo.
  
  
  RESULTS
  Mesothelial cells were present in all patient dialysates except that of a single patient's Glu dialysate. The number of MC drained was similar with both solutions. After the initial culture reached confluence, MC were identified in 14 and 12 patients receiving Ico and Glu, respectively. However, in 1 patient using Ico and in 2 using Glu, the MC count at this stage was so low that further subculture could not be performed. Cells from Ico-derived solutions exhibited a higher degree of proliferation than cells from Glu-derived solutions. The morphology of MC was also different. Cells from drained effluent were typical in 11 patients using Glu solution in contrast with 14 patients using Ico. At confluence, the percentages of typical appearance were 50% and 92.9% (p < 0.05) in Glu and Ico respectively.
  
  
  CONCLUSIONS
  Mesothelial cells taken from icodextrin effluent show a greater proliferation ex vivo than those taken from glucose effluent.
authors.affiliation: Servicio de Nefrología, Hospital Universitario La Paz, Madrid, Spain. mabajo@hulp.insalud.es
authors.forename: M A
authors.initials: MA
authors.lastname: Bajo
authors.forename: R
authors.initials: R
authors.lastname: Selgas
authors.forename: M A
authors.initials: MA
authors.lastname: Castro
authors.forename: G
authors.initials: G
authors.lastname: del Peso
authors.forename: C
authors.initials: C
authors.lastname: Diaz
authors.forename: J A
authors.initials: JA
authors.lastname: Sánchez-Tomero
authors.forename: M
authors.initials: M
authors.lastname: Fernandez de Castro
authors.forename: V
authors.initials: V
authors.lastname: Alvarez
authors.forename: A
authors.initials: A
authors.lastname: Corbí
mesh_terms: D002455:Cell Division / Q000187:drug effects; D015314:Dialysis Solutions / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects* / Q000502:physiology*; D005260:Female; D005936:Glucans / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D000077607:Icodextrin; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies
chemical_list: D015314:Dialysis Solutions; D005936:Glucans; D000077607:Icodextrin; D005947:Glucose