Hydrolase and sialyltransferase activities of trypanosoma cruzi trans-sialidase towards NeuAc-alpha-2,3-gal-Gal-beta-O-PNP.
NeuAc-alpha-2,3-Gal-beta-O-PNP has been synthesised and its ability to act as a substrate for the hydrolase and transferase activities of Trypanosoma cruzi trans-sialidase have been investigated. The turn-over of this compound shows marked differences from the behaviour of NeuAc-MU. In addition, distinct differences in the action of T. cruzi trans-sialidase and Clostridium perfringens neuraminidase on NeuAc-alpha-2,3-Gal-beta-O-PNP were apparent.
Harrison
J A
JA
School of Chemistry, University of St. Andrews, UK.
Kartha
K P
KP
Turnbull
W B
WB
Scheuerl
S L
SL
Naismith
J H
JH
Schenkman
S
S
Field
R A
RA
D003016:Clostridium perfringens; D004187:Disaccharides / Q000737:chemistry / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D006867:Hydrolases / Q000737:chemistry / Q000378:metabolism; D015335:Molecular Probes / Q000138:chemical synthesis / Q000737:chemistry; D019158:N-Acetylneuraminic Acid / Q000737:chemistry / Q000378:metabolism; D009439:Neuraminidase / Q000378:metabolism*; D009596:Nitrophenols / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D013057:Spectrum Analysis / Q000379:methods; D013379:Substrate Specificity; D014166:Transferases / Q000737:chemistry / Q000378:metabolism
D004187:Disaccharides; D006023:Glycoproteins; D015335:Molecular Probes; C425225:NeuAc-2,3-Gal-O-PNP; D009596:Nitrophenols; D014166:Transferases; D006867:Hydrolases; C069715:trans-sialidase; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid