title: PAF-mediated Ca2+ influx in human neutrophils occurs via store-operated mechanisms.
abstract: Many inflammatory mediators activate neutrophils (PMN) partly by increasing cytosolic calcium concentration ([Ca2+]i). Modulation of PMN [Ca2+]i might therefore be useful in regulating inflammation after shock or sepsis. The hemodynamic effects of traditional Ca2+ channel blockade, however, could endanger unstable patients. Store-operated calcium influx (SOCI) is known now to contribute to Ca2+ flux in "nonexcitable" cells. Therefore, we studied the role of SOCI in human PMN responses to the proinflammatory ligand PAF. PMN [Ca2+]i was studied by spectrofluorometry with and without external calcium. We studied the effects o
authors.affiliation: Department of Surgery, University of Medicine and Dentistry of New Jersey/New Jersey Medical School, Newark 07103, USA. hausercj@UMDNJ.edu
authors.forename: C J
authors.initials: CJ
authors.lastname: Hauser
authors.forename: Z
authors.initials: Z
authors.lastname: Fekete
authors.forename: J M
authors.initials: JM
authors.lastname: Adams
authors.forename: M
authors.initials: M
authors.lastname: Garced
authors.forename: D H
authors.initials: DH
authors.lastname: Livingston
authors.forename: E A
authors.initials: EA
authors.lastname: Deitch
mesh_terms: D002118:Calcium / Q000502:physiology*; D002478:Cells, Cultured; D006801:Humans; D017136:Ion Transport / Q000187:drug effects / Q000502:physiology; D009504:Neutrophils / Q000502:physiology*; D010972:Platelet Activating Factor / Q000494:pharmacology*; D015398:Signal Transduction
chemical_list: D010972:Platelet Activating Factor; D002118:Calcium