Increase in hypoxanthine-guanine phosphoribosyl transferase gene mutations by exposure to electric field.
Previously, we reported that exposure to extremely low frequency magnetic field (400 mT) increased in hypoxanthine-guanine phosphoribosyl transferase (HPRT) gene mutations. However, it is unclear these mutations were induced by magnetic field (MF), electric field (EF), or both. To explore this question, a new exposure apparatus for EF was manufactured. We observed an increase in HPRT gene mutations in Chinese hamster ovary (CHO) cells after exposure to EF (10 V/m, 60 Hz) for 10 h. The mutant frequency by EF-exposure was an approximate 2-fold of that by sham-exposure. Our data suggest that the mutations induced by exposure of cells to the variable magnetic field at 400 mT may be, in part, due to the induced EF.
Ding
G R
GR
Department of Radiation Medicine, The Fourth Military Medical University, Xi'an, China.
Wake
K
K
Taki
M
M
Miyakoshi
J
J
D000818:Animals; D016466:CHO Cells / Q000201:enzymology / Q000528:radiation effects; D006224:Cricetinae; D004560:Electricity; D004574:Electromagnetic Fields / Q000009:adverse effects*; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D009152:Mutagenicity Tests / Q000295:instrumentation / Q000379:methods*
D007041:Hypoxanthine Phosphoribosyltransferase