Peripheral neuroglial death induced by cisplatin administration in newborn rats.
To determine the target of cytotoxicity of cisplatin (CDDP), we injected newborn rats with 2 mg/kg CDDP and examined the trigeminal ganglion for possible cell death. A nick translation method for DNA fragmentation revealed CDDP-induced glial cell death. DNA fragmentation was detected in both Schwann cells and satellite cells. Satellite cell death was observed as early as 0.5 day after injection, most frequent at 1-3 days and subsided thereafter. The incidence of neuronal death was very low and comparable to that observed in vehicle control rats. CDDP has selective toxicity to peripheral glial cells, though the damage did not culminate in cell death in adults. The glial toxicity may contribute to clinical symptoms of CDDP neuropathy.
Sugimoto
T
T
Department of Oral Anatomy, Okayama University Dental School, Japan.
Takeyama
A
A
Fujita
M
M
Ichikawa
H
H
Takano-Yamamoto
T
T
D000818:Animals; D000831:Animals, Newborn; D000970:Antineoplastic Agents / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D002945:Cisplatin / Q000494:pharmacology*; D053938:DNA Fragmentation; D005260:Female; D008297:Male; D009457:Neuroglia / Q000187:drug effects / Q000473:pathology; D009474:Neurons / Q000187:drug effects*; D009836:Oligodendroglia / Q000187:drug effects* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000187:drug effects* / Q000473:pathology; D012668:Trigeminal Ganglion / Q000187:drug effects / Q000473:pathology
D000970:Antineoplastic Agents; D002945:Cisplatin