Roles of Se and NO in apoptosis of hepatoma cells.
Mice inoculated with hepatoma cell (H22) suspension subcutaneously at their right axilla were administered orally with kappa-selenocarrageenan (Se) solution, the inoculated hepatoma's growth was suppressed. Different concentrations of Se solution added in human hepatoma cell line culture could inhibit proliferation and induce apoptosis in hepatoma cells. Meanwhile Se solution could increase the activity of glutathione peroxidase (GSH-Px) in the mice's plasma and the content of NO in the mice's sera and the hepatoma cell culture supernatant as well. Therefore, apoptosis in hepatoma cell induced by Se solution may be associated with the increase in antioxidative activity, the suppression free radical's intervention, and the excessive release of NO by stress.
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Department of Biochemistry, Medical college of Fudan University, Shanghai, People's Republic of China. slliu@shmu.edu.cn
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Chen
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D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002351:Carrageenan / Q000494:pharmacology; D002452:Cell Count; D002847:Chromatography, Agarose; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D008114:Liver Neoplasms, Experimental / Q000473:pathology*; D051379:Mice; D009569:Nitric Oxide / Q000502:physiology*; D016566:Organoselenium Compounds / Q000494:pharmacology; D012643:Selenium / Q000502:physiology*; D014407:Tumor Cells, Cultured
D005456:Fluorescent Dyes; D016566:Organoselenium Compounds; C079969:kappa-selenocarrageenan; D009569:Nitric Oxide; D002351:Carrageenan; D005979:Glutathione Peroxidase; D012643:Selenium