title: Selective reaction to a flipping cytidine of the duplex DNA mediated by triple helix formation.
abstract: A new nucleoside derivative (2) with a butyl spacer between the sugar part and the 2-amino-6-vinylpurine motif has been synthesized. The triplex-forming oligodeoxynucleotide incorporating 2 has achieved strand- and cytidine-selective cross-linking reaction to the G-C target site mediated by triple helix formation. It has been suggested that 2 reacts with a flipping cytidine at the target site.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: F
authors.initials: F
authors.lastname: Nagatsugi
authors.forename: D
authors.initials: D
authors.lastname: Usui
authors.forename: T
authors.initials: T
authors.lastname: Kawasaki
authors.forename: M
authors.initials: M
authors.lastname: Maeda
authors.forename: S
authors.initials: S
authors.lastname: Sasaki
mesh_terms: D020137:Base Pair Mismatch; D020029:Base Pairing; D003432:Cross-Linking Reagents / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D003562:Cytidine / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D004586:Electrophoresis; D009690:Nucleic Acid Conformation; D009705:Nucleosides / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D013329:Structure-Activity Relationship; D013696:Temperature
chemical_list: D003432:Cross-Linking Reagents; D009705:Nucleosides; D009838:Oligodeoxyribonucleotides; D003562:Cytidine; D004247:DNA