title: Distribution of peptidergic nerve fibres in the guinea pig trabecular meshwork.
abstract: A quantitative analysis of peptidergic nerve fibres located in the trabecular meshwork of the guinea pig has been performed. Our results confirm that this structure contains VIP-, NPY- and substance P-like immunoreactivity as major neurotransmitters. These findings were obtained using immunohistochemical techniques. For this purpose serial sections of the eye were stained by immunohistochemistry for each of three neurotransmitters and stained sections were analysed by quantitative image analysis. Our findings demonstrate that SP-positive, NPY-positive and VIP-positive nerve fibres occupy 11.2, 4.9 and 2.4%, respectively, of the observed area (expressed as conventional units, C.U.) in the trabecular meshwork of the guinea pig eye. It is relevant to emphasize that the area containing these three types of peptidergic nerve fibres appears to be large (18.5 +/- 6.6 C.U.) in proportion to the total observed area. The innervation of the drainage angle of the guinea pig eye has been well described by many authors. This is the first study to report quantitative measurements of three types of peptidergic nerve fibres identified and measured in this area. The presence of these three neurotransmitters in the trabecular meshwork of guinea pig eye suggests their possible participation in aqueous humor regulation.
authors.affiliation: Department of Cardiovascular and Respiratory Sciences, University of Rome La Sapienza, Rome, Italy. cavallotti@uniroma1.it
authors.forename: C
authors.initials: C
authors.lastname: Cavallotti
authors.forename: M
authors.initials: M
authors.lastname: Artico
authors.forename: N
authors.initials: N
authors.lastname: Pescosolido
authors.forename: F M
authors.initials: FM
authors.lastname: Tranquilli Leali
authors.forename: E
authors.initials: E
authors.lastname: Pacella
mesh_terms: D000818:Animals; D006168:Guinea Pigs / Q000033:anatomy & histology*; D007150:Immunohistochemistry; D009412:Nerve Fibers / Q000648:ultrastructure*; D014129:Trabecular Meshwork / Q000033:anatomy & histology / Q000294:innervation*