Characterization of four isolates of Bombyx mori nucleopolyhedrovirus.
Bombyx mori nucleopolyhedroviruses (BmNPVs), isolated from a sericultural Korean farm, were purified and characterized by their DNA restriction pattern, virus replication, polyhedra production and gene structures. The EcoR I and Sal I fragments showed similar overall patterns with minor difference but distinguishable patterns in each isolate. There was no significant difference in the virus replication pattern, yield of total polyhedra production and polyhedra morphology, but the yield of released polyhedra by BmNPV-K1 in Bm5 cells was 2 to 5 times higher than that of other isolates. In comparative studies of p10 gene, BmNPV-K1 and K3 had same structure and they encoded a protein consisting of 94 amino acids. Although BmNPV-K2 encoded the same length of amino acids with BmNPV-K1 and K3, it had different structure, and BmNPV-K4 had the p10 gene encoding 70 amino acids.
Hong
H K
HK
Division of Applied Biology and Chemistry, College of Agriculture and Life Sciences, Seoul National University, Suwon, Korea.
Woo
S D
SD
Choi
J Y
JY
Lee
H K
HK
Kim
M H
MH
Je
Y H
YH
Kang
S K
SK
D000595:Amino Acid Sequence; D000818:Animals; D012831:Bombyx / Q000821:virology*; D002478:Cells, Cultured; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D000077183:Occlusion Body Matrix Proteins; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000648:ultrastructure; D015678:Viral Structural Proteins; D014779:Virus Replication
D000077183:Occlusion Body Matrix Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins; C009829:polyhedrin protein, Nucleopolyhedrovirus; C078072:p10 protein, Baculovirus; D015246:Deoxyribonuclease EcoRI; D015252:Deoxyribonucleases, Type II Site-Specific; C029792:GTCGAC-specific type II deoxyribonucleases