[Correction of measurement errors in the ELISA test].
The authors followed the influence of the arrangement of measured and control samples on microtitration plate on the ELISA test results by quantitative evaluation of rabies antibodies titres in human blood serum. They performed the test under identical conditions in all microtitration plate wells. They calculated the result according to five calibration curves--four of them were obtained by different positioning of the control positive and negative sera and the fifth curve was obtained from average values. The results from the four various positional calibration curves were significantly statistically different from the average. However, the average calibration curve--obtained from four measurements--led to identical value of rabies antibodies in cases with different dilutions of sera. The authors propose to follow some principles of measured and control samples arrangement on microtitration plates in order to minimalize the errors, caused by their dishomogeneity. (Tab. 2, Fig. 3, Ref. 10.)
Suli
J
J
Department of Infectology and Tropical Animal Medicine, University of Animal Medicine, Kosice. janos@dodo.sk
Benisek
Z
Z
Svrcek
S
S
Ondrejkova
A
A
Ondrejka
R
R
D000914:Antibodies, Viral / Q000097:blood*; D002138:Calibration; D003951:Diagnostic Errors; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards; D006801:Humans; D011820:Rabies virus / Q000276:immunology*
D000914:Antibodies, Viral
Korekcia chýb merania v teste ELISA.