title: Cytotoxicity of mineral trioxide aggregate using human periodontal ligament fibroblasts.
abstract: The purpose of the present study was to compare the cytotoxicity of mineral trioxide aggregate (MTA) to other commonly used retrofilling materials, Super-EBA and amalgam. This was accomplished using a cell viability assay for mitochondrial dehydrogenase activity in human periodontal ligament fibroblasts after 24-hr exposure to extracts of varying concentrations of the test materials, in both freshly mixed and 24-hr set states. Methyl methacrylate 2% (vol/vol) served as the positive control, and complete culture medium served as the negative control. Differences in mean cell viability values were assessed by ANOVA (p < 0.05). In the freshly mixed state, the sequence of toxicity was amalgam > Super-EBA > MTA. In the 24-hr set state the sequence of toxicity at a low extract concentration was Super-EBA > MTA, amalgam, and Super-EBA > amalgam > MTA at a higher extract concentration. This study supports the use of MTA in the root-end environment.
authors.affiliation: Department of Biologic and Diagnostic Sciences, Division of Endodontics, University of Tennessee College of Dentistry, 875 Union Avenue, Memphis, TN 38163, USA.
authors.forename: K
authors.initials: K
authors.lastname: Keiser
authors.forename: C C
authors.initials: CC
authors.lastname: Johnson
authors.forename: D A
authors.initials: DA
authors.lastname: Tipton
mesh_terms: D017607:Aluminum Compounds / Q000633:toxicity*; D000704:Analysis of Variance; D017610:Calcium Compounds / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media; D003723:Dental Amalgam / Q000633:toxicity; D017220:Dentin-Bonding Agents / Q000633:toxicity; D004338:Drug Combinations; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D020366:Methylmethacrylate / Q000633:toxicity; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology; D010087:Oxides / Q000633:toxicity*; D010088:Oxidoreductases / Q000187:drug effects; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects*; D012184:Retrograde Obturation; D012387:Root Canal Filling Materials / Q000633:toxicity*; D017640:Silicates / Q000633:toxicity*; D013997:Time Factors
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D003470:Culture Media; D017220:Dentin-Bonding Agents; D004338:Drug Combinations; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate; D020366:Methylmethacrylate; D003723:Dental Amalgam; C084074:Super EBA; D010088:Oxidoreductases