Muscle glycogen stores and meat quality as affected by strategic finishing feeding of slaughter pigs.
The aim of the present study was to investigate whether muscle glycogen stores in slaughter pigs could be decreased through strategic finishing feeding before slaughter. Moreover, preliminary meat quality traits were measured to see whether such a regulation of muscle glycogen stores affected ultimate pH, color, and tenderness in the meat. The strategic finishing feeding was carried out the last 3 wk prior to slaughter. Seven experimental groups with eight animals per group were fed diets low in digestible carbohydrates. A control group with four animals was fed a traditional grower-finishing diet. The muscle glycogen stores were reduced in longissimus muscle (LM) 11 to 26% at the time of slaughter in pigs that were fed the experimental diets compared with the control group. Meat quality measured as ultimate pH and color on LM muscle in half the pigs 24 h postmortem showed that ultimate pH in LM was not affected by the reduction in glycogen stores in the muscles from pigs fed any of the experimental diets. However, the meat from pigs fed the experimental diets was darker than the meat from pigs that were fed the control diet with two of the experimental diets, resulting in significantly lower L* values. Activities of key enzymes in the glycolytic pathway, glycogen phoshorylase a and b, phosphofructokinase, and the fatty acid oxidative pathway, beta-hydrozyacyl-CoA-dehydrogenase, were not affected by the strategic feeding. In contrast, the activity of the proteolytic enzyme calpain as well as its inhibitor calpastatin was influenced by the strategic feeding. Lower activity of mu-calpain and greater activity of calpastatin in the muscle samples from the strategically fed pigs indicate a lesser muscle protein degradation in the muscles compared with muscles of control animals. The present study showed that the muscle glycogen stores in slaughter pigs can be reduced at the time of slaughter through strategic finishing feeding with diets low in digestible carbohydrate without compromising growth rate.
Rosenvold
K
K
Department of Animal Product Quality, Danish Institute of Agricultural Sciences, Tjele.
Petersen
J S
JS
Lwerke
H N
HN
Jensen
S K
SK
Therkildsen
M
M
Karlsson
A H
AH
Møller
H S
HS
Andersen
H J
HJ
D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000032:analysis; D000821:Animal Feed; D000818:Animals; D001706:Biopsy / Q000662:veterinary; D002135:Calcium-Binding Proteins / Q000032:analysis; D002154:Calpain / Q000032:analysis; D003116:Color; D015853:Cysteine Proteinase Inhibitors / Q000032:analysis; D004040:Dietary Carbohydrates / Q000378:metabolism / Q000494:pharmacology; D005260:Female; D006003:Glycogen / Q000032:analysis / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008460:Meat / Q000592:standards*; D018482:Muscle, Skeletal / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism*; D009211:Myoglobin / Q000032:analysis; D010732:Phosphofructokinase-1 / Q000032:analysis; D006005:Phosphorylases / Q000032:analysis; D013552:Swine / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*
D002135:Calcium-Binding Proteins; D015853:Cysteine Proteinase Inhibitors; D004040:Dietary Carbohydrates; D009211:Myoglobin; C033668:calpastatin; D006003:Glycogen; D015094:3-Hydroxyacyl CoA Dehydrogenases; D006005:Phosphorylases; D010732:Phosphofructokinase-1; D002154:Calpain