Expression of intercellular adhesion molecule-1 induced by high glucose concentrations in human aortic endothelial cells.
We examined the effects of high glucose concentrations on the expression of adhesion molecules in human aortic endothelial cells. Expression levels of both mRNA and protein of intercellular adhesion molecule-1 (ICAM-1) were increased after incubation of endothelial cells with 30 mM glucose for 24 h. The effect of glucose on ICAM-1 was concentration dependent, partially attributable to osmolarity, and enhanced by glycated-collagen. Staurosporine (10 nM), epalrestat (10 microM) suppressed the expression of ICAM-1 mRNA and protein induced by high glucose to variable extents. Aminoguanidine (50 mM) suppressed the expression of ICAM-1 protein. It is thought that soluble ICAM-1 protein is produced by shedding in human aortic endothelial cells because RNA for the soluble form of ICAM-1 formed by variant splicing has not been detected. These results show that glucose is an important determinant of ICAM-1 expression in endothelial cells, and suggest that ICAM-1 molecules induced by hyperglycemia may contribute to the development of atherosclerosis in diabetes mellitus.
Kado
S
S
Department of Internal Medicine 5, Tokyo Medical University, Inashiki, Ibaraki, Japan. s-kado@tokyo-med.ac.jp
Wakatsuki
T
T
Yamamoto
M
M
Nagata
N
N
D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors; D001011:Aorta / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D005947:Glucose / Q000494:pharmacology*; D006146:Guanidines / Q000494:pharmacology; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D052250:Nitric Oxide Synthase Type III; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012236:Rhodanine / Q000031:analogs & derivatives* / Q000494:pharmacology; D019311:Staurosporine / Q000494:pharmacology; D053778:Thiazolidines
D004791:Enzyme Inhibitors; D006146:Guanidines; D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; D053778:Thiazolidines; D018799:Intercellular Adhesion Molecule-1; C038131:epalrestat; D012236:Rhodanine; D003094:Collagen; D000449:Aldehyde Reductase; C496314:NOS3 protein, human; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III; D019311:Staurosporine; D005947:Glucose; C004479:pimagedine