Particle-mediated gene therapy of wounds.
Gene therapy is becoming a reality, and it is a particularly attractive approach for wound healing, because the wound site is often exposed, the treatment and condition should be transient, and gene products such as growth factors and cytokines suffer from problems with bioavailability and stability. Among the techniques for gene delivery to the wound site, particle-mediated bombardment with a device called the gene gun has become an important developmental tool. This instrument has been used in numerous examples of wound gene therapy with growth factors or their receptors in the last decade. Among the advantages of particle-mediated bombardment are ease and speed of preparation of the delivery vehicle, the stability of the DNA preparation, the absence of (viral) antigens, the ability to target the projectiles to different tissue depths and areas, and the rapid shedding of both particles and DNA if they are targeted to the epidermis. Clinical application of the technology remains limited by the relatively low efficiency of the method, the potential tissue damage created by impact of the particles, and the coverage area. The gene gun can also be used to facilitate the discovery and validation of gene products as wound healing agents.
Davidson
J M
JM
Department of Pathology, Vanderbilt University School of Medicine, C-3321 Medical Center North, Nashville, TN 37232-2561, USA. jeff.davidson@vanderbilt.edu
Krieg
T
T
Eming
S A
SA
D004337:Drug Carriers; D004867:Equipment Design; D018014:Gene Transfer Techniques / Q000295:instrumentation*; D015316:Genetic Therapy / Q000379:methods*; D006133:Growth Substances / Q000008:administration & dosage* / Q000235:genetics*; D006801:Humans; D012680:Sensitivity and Specificity; D014945:Wound Healing / Q000235:genetics* / Q000502:physiology; D014947:Wounds and Injuries / Q000628:therapy*
D004337:Drug Carriers; D006133:Growth Substances