Colloidal gold as an electrochemical label of streptavidin-biotin interaction.
A new electrochemical method to monitor biotin-streptavidin interaction, based on the use of colloidal gold as an electrochemical label, is investigated. Biotinylated albumin is adsorbed on the pretreated surface of a carbon paste electrode (CPE). This modified electrode is immersed in colloidal gold-streptavidin labelled solutions. Adsorptive voltammetry is used to monitor colloidal gold bound to streptavidin, obtaining a good reproducibility of the analytical signal (R.S.D. = 3.3%). A linear relationship between peak current and streptavidin concentration from 2.5 x 10(-9) to 2.5 x 10(-5) M is obtained when a sequential competitive assay between streptavidin and colloidal gold-labelled streptavidin is carried out. On the other hand, the adsorption of streptavidin on the electrode surface was performed, followed by the reaction with biotinylated albumin labelled with colloidal gold. In this way, a linear relationship between peak current and colloidal gold labelled biotinylated albumin concentration is achieved with a limit of detection of 7.3 x 10(9) gold particles per ml (5.29 x 10(-9) M in biotin).
González-García
M B
MB
Departamento de Química Física y Analítica, Universidad de Oviedo, Asturias, Spain.
Fernández-Sánchez
C
C
Costa-García
A
A
D000818:Animals; D015374:Biosensing Techniques; D001710:Biotin; D002417:Cattle; D004563:Electrochemistry; D017979:Gold Colloid; D012710:Serum Albumin, Bovine / Q000032:analysis; D019809:Streptavidin
D017979:Gold Colloid; D012710:Serum Albumin, Bovine; D001710:Biotin; D019809:Streptavidin