Costimulatory effect of Fas in mouse T lymphocytes.
To induce proper immune responses, T lymphocytes require two types of stimuli, antigen-specific and costimulatory signals. Among costimulatory molecules, CD28-engagement promotes the survival and proliferation of both naive and memory T cells. In addition, it is now believed that Fas may play a role in T cell activation in the human system. It is, however, controversial whether Fas can act as a costimulatory signal in the murine system. Thus, we investigated fundamental differences in the capacity to induce proliferation of T cells between Fas and CD28 in mice. Fas-mediated T cell proliferation was observed only with a full mitogenic dose of anti-CD3 antibodies, whereas CD28 engagement was able to enhance T cell proliferation in the presence of a suboptimal level of anti-CD3 antibody. Furthermore, Fas-engaged T cells showed faster response in the upregulation of CD25 and CD69 expression than CD28-engaged ones. Here, we report that Fas might play a role in mature T cell activation in the mouse system through a different mechanism from that in CD28 costimulation.
Chun
D H
DH
Department of Pathology, Seoul National University School of Medicine, Korea.
Jung
K C
KC
Park
W S
WS
Lee
I S
IS
Choi
W J
WJ
Kim
C J
CJ
Park
S H
SH
Bae
Y
Y
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D015703:Antigens, CD / Q000494:pharmacology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000494:pharmacology; D018106:CD28 Antigens / Q000494:pharmacology; D017252:CD3 Complex / Q000276:immunology / Q000502:physiology; D018929:Cell Culture Techniques; D005260:Female; D016130:Immunophenotyping; D037181:Lectins, C-Type; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice / Q000276:immunology*; D008810:Mice, Inbred C57BL; D015375:Receptors, Interleukin-2 / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013601:T-Lymphocytes / Q000276:immunology*; D015854:Up-Regulation / Q000187:drug effects; D019014:fas Receptor / Q000494:pharmacology*; D000099192:CD69 Antigens
D000906:Antibodies; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D018106:CD28 Antigens; D017252:CD3 Complex; D037181:Lectins, C-Type; D015375:Receptors, Interleukin-2; D019014:fas Receptor; D000099192:CD69 Antigens