Synthesis of non-nucleoside triphosphate analogues, a new type of substrates for terminal deoxynucleotidyl transferase.
A series of non-nucleoside triphosphate analogues were synthesized. In place of the nucleoside fragment, substituents bearing aromatic groups were introduced; the triphosphate component was replaced at alpha, beta, or gamma-positions by phosphonates. Alpha-[2-N-(9-Fluorenylmethoxycarbonyl)aminoethylphosphonyl]-beta,gamma-difluoromethylenediphosphonate (IIc) revealed the best substrate properties toward terminal deoxynucleotidyl transferase.
Arzumanov
A A
AA
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia Federation.
Victorova
L S
LS
Jasko
M V
MV
D004253:DNA Nucleotidylexotransferase / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D009943:Organophosphorus Compounds / Q000138:chemical synthesis* / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D013379:Substrate Specificity
D009943:Organophosphorus Compounds; D004253:DNA Nucleotidylexotransferase