Cytotoxic effect of four root filling materials.
The aim of this study was to evaluate the cytotoxic effect of four root canal sealers: AH26, AH Plus, Diaket and Apexit. In the experiment two cell lines, human cervical carcinoma (HeLa) cells and mouse skin fibroblasts (L929), were used. Under aseptic conditions, the sealers were prepared according to the manufacturers' directions, and 0.01 mL of each material was placed in a 24-well plate. The sealers were covered with cell suspension. The cytotoxicity was estimated by determining the number of viable cells by a light microscope, as well as the total number of cells 24 h, 48 h and 120 h after the treatment with mentioned materials. The results obtained in this study showed the high cytotoxcity of the new AH Plus root canal sealer, which was shown to be equally or more toxic to the standard AH26 and Diaket materials. Apexit was the least toxic sealer.
Miletić
I
I
Department of Dental Pathology, School of Dentistry, University of Zagreb, Zagreb, Croatia.
Anić
I
I
Karlović
Z
Z
Marsan
T
T
Pezelj-Ribarić
S
S
Osmak
M
M
D000818:Animals; D001729:Bismuth / Q000633:toxicity; D002126:Calcium Hydroxide / Q000633:toxicity; D002452:Cell Count; D002460:Cell Line; D048429:Cell Size / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004338:Drug Combinations; D004853:Epoxy Resins / Q000633:toxicity; D005347:Fibroblasts / Q000187:drug effects; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D008709:Methenamine / Q000633:toxicity; D051379:Mice; D011145:Polyvinyls / Q000633:toxicity; D012387:Root Canal Filling Materials / Q000633:toxicity*; D012834:Silver / Q000633:toxicity; D012867:Skin / Q000166:cytology / Q000187:drug effects; D013223:Statistics as Topic; D013997:Time Factors; D014025:Titanium / Q000633:toxicity; D014407:Tumor Cells, Cultured; D015034:Zinc Oxide / Q000633:toxicity
C074418:Apexit; D004338:Drug Combinations; D004853:Epoxy Resins; D011145:Polyvinyls; D012387:Root Canal Filling Materials; C534916:epoxy resin-based root canal sealer; D012834:Silver; C028044:epoxy resin AH-26; C014457:bismuth phosphate, copolymers of vinyl acetate, dichlorophen, propionyl-acetophenone, triethanolamine, vinyl chloride, vinylisobutyl ether, zinc oxide drug combination; D014025:Titanium; D008709:Methenamine; D002126:Calcium Hydroxide; D015034:Zinc Oxide; D001729:Bismuth