Immunohistochemical evaluation of endomannosidase distribution in rat tissues: evidence for cell type-specific expression.
Asparagine-linked oligosaccharides of glycoproteins are subject to a series of trimming reactions by glucosidases and mannosidases in the endoplasmic reticulum which result in the removal of all three glucose residues and several of the nine mannose residues. At present, endomannosidase represents the only processing enzyme which cleaves internally and provides an alternate deglucosylation pathway. However, in contrast to the endoplasmic reticulum residential proteins glucosidase I and II, endomannosidase is primarily situated in the Golgi apparatus of rat liver hepatocytes and hepatocyte cell lines. We have performed a confocal immunohistochemical study to investigate endomannosidase in various rat tissues and used a monoclonal antibody against Golgi mannosidase II as a marker for the Golgi apparatus. Although immunofluorescence for both endomannosidase and Golgi mannosidase II was detectable in the epithelia of many tissues, renal proximal tubular cells, cortex and medulla of adrenal gland, gastric mucosa, and Leydig cells of testis were unreactive for endomannosidase. Furthermore, the endothelia in all studied tissues were unreactive for endomannosidase but positive for Golgi mannosidase II. It is concluded that by immunohistochemistry endomannosidase exhibits a cell type-specific expression in rat tissues.
Dong
Z
Z
Department of Pathology, University of Zürich, Switzerland.
Zuber
C
C
Spiro
M J
MJ
Spiro
R G
RG
Roth
J
J
D000311:Adrenal Glands / Q000201:enzymology; D000818:Animals; D000911:Antibodies, Monoclonal; D001921:Brain / Q000201:enzymology; D003106:Colon / Q000201:enzymology; D004727:Endothelium / Q000166:cytology / Q000201:enzymology; D004847:Epithelial Cells / Q000201:enzymology; D005455:Fluorescent Antibody Technique; D005753:Gastric Mucosa / Q000201:enzymology; D006056:Golgi Apparatus / Q000201:enzymology*; D007583:Jejunum / Q000201:enzymology; D007687:Kidney Tubules, Proximal / Q000201:enzymology; D008099:Liver / Q000201:enzymology*; D008297:Male; D008361:Mannosidases / Q000032:analysis* / Q000276:immunology; D010179:Pancreas / Q000201:enzymology; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000201:enzymology; D013961:Thyroid Gland / Q000201:enzymology
D000911:Antibodies, Monoclonal; D008361:Mannosidases; C051635:endo-alpha-D-mannosidase; C038829:mannosyl-oligosaccharide 1,3 - 1,6-alpha-mannosidase