Amplification and analysis of DNA flanking known sequences of a novel herpesvirus from green turtles with fibropapilloma Brief report.
A 1,632-bp fragment, flanking the original 483-bp region of the DNA polymerase gene of a novel herpesvirus found in tissues of green turtles (Chelonia mydas) with fibropapilloma, was amplified from the circularized EcoRI-cut DNA extracted from tumor tissues by inverse PCR. The resultant 2,019-bp partial sequence of the DNA polymerase gene of the newfound herpesvirus, including the original 483-bp region, showed a high degree of homology at both the nucleotide and amino acid levels with that of other human and animal herpesviruses. Phylogenetic analysis confirmed that this novel herpesvirus belonged to the Alphaherpesvirinae subfamily.
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Retrovirology Research Laboratory, Pacific Biomedical Research Center, University of Hawaii at Manoa, Honolulu 96816, USA.
Lu
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Nerurkar
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Yanagihara
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D018138:Alphaherpesvirinae / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000032:analysis; D004259:DNA-Directed DNA Polymerase / Q000235:genetics; D005814:Genes, Viral; D006566:Herpesviridae Infections / Q000662:veterinary*; D008969:Molecular Sequence Data; D010212:Papilloma / Q000662:veterinary*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012878:Skin Neoplasms / Q000662:veterinary*; D014426:Turtles / Q000821:virology*
D004279:DNA, Viral; D004259:DNA-Directed DNA Polymerase