Anti-apoptotic role of Bfl-1 in staurosporine-treated B-lymphoblastic cells.
To examine the relationship between the expression level of Bfl-1 and the susceptibility to staurosporine-induced apoptosis in B-lymphoblastic cells, we tested cell survival in 4 cell lines: 2 that are Bfl-1-nonexpressing (Reh and JM-1) and 2 that are Bfl-1-expressing (IM-9 and Wil2-NS). Reh and JM-1 showed apoptosis levels of 62% (Reh) and 30% (JM-1) as early as 3 hours after treatment with staurosporine, whereas IM-9 and Wil2-NS showed apoptosis levels of only 40% and 26%, respectively, even after 1 day of treatment with staurosporine. Either induced expression of Bfl-1 with 12-o-tetradecanoyl phorbol-13-acetate or exogenous expression of Bfl-1 by transfection in Reh cells promoted cell survival. These results suggest that expression of Bfl-1 contributes to regulating apoptosis in the B-cell lines we examined.
Shim
Y H
YH
Department of Pathology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.
Byun
E K
EK
Lee
M J
MJ
Huh
J
J
Kim
C W
CW
D017209:Apoptosis / Q000187:drug effects*; D001402:B-Lymphocytes / Q000187:drug effects* / Q000502:physiology; D015870:Gene Expression; D019254:Genes, bcl-2; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000502:physiology; D006801:Humans; D015778:Minor Histocompatibility Antigens; D005810:Multigene Family / Q000235:genetics; D011506:Proteins / Q000235:genetics / Q000494:pharmacology* / Q000502:physiology; D019253:Proto-Oncogene Proteins c-bcl-2; D019311:Staurosporine / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
C096816:BCL2-related protein A1; D015778:Minor Histocompatibility Antigens; D011506:Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D019311:Staurosporine