[Homogenous low-density lipoprotein assay in type III hyperlipoproteinemia].
To investigate whether homogenous analysis of serum low-density lipoprotein cholesterol(LDL-C) was applicable in type III hyperlipoproteinemic subjects, 3 reagents were used to estimate LDL-C in 2 cases with apoE 2/2 phenotype. Each measurements were compared to LDL-C levels by ultracentrifugation(density: 1.019-1.063). LDL-C levels by homogenous analysis with any of the reagents were higher than those by ultracentrifugation, and the difference was varied between the reagents: +14-24% by LDL-EX, +29-55% by Cholestest LDL, and +89-115% by Determiner LDL-C. The cross-reaction by the reagents to intermediate density lipoprotein(IDL) and very low density lipoprotein(VLDL) cholesterol was studied in a case; 22% of cholesterol in these fractions was measured by LDL-EX, 32% by Cholestest LDL, and 110% by Determiner LDL-C. A part of the sample serum was stored at -60 degrees C for several days and melted for re-analysis of homogenous LDL-C levels. After the freezing process, IDL fraction pattern on electrophoresis had been changed and homogenous LDL-C levels by all reagents were different from the measurements of native serum. These results indicated that using reagents with low cross-reaction to IDL and VLDL fractions and careful sample handling are important in homogenous LDL-C analysis in type III hyperlipoproteinemia.
Waki
M
M
Internal Medicine, Shimada Municipal Hospital, Shimada 427-8502.
Amano
T
T
D000368:Aged; D001774:Blood Chemical Analysis / Q000379:methods*; D008078:Cholesterol, LDL / Q000097:blood*; D005260:Female; D006801:Humans; D006952:Hyperlipoproteinemia Type III / Q000097:blood*; D007202:Indicators and Reagents; D008297:Male; D008875:Middle Aged; D013048:Specimen Handling
D008078:Cholesterol, LDL; D007202:Indicators and Reagents