title: Heat shock response is associated with protection against acute interstitial pancreatitis in rats.
abstract: We recently reported that hyperthermia induces pancreatic expression of heat shock proteins (HSPs), particularly HSP70 isoforms, and protects against cerulein pancreatitis. We have now studied whether a double hyperthermia amplifies these effects and whether hyperthermia also protects against dibutyltin dichloride (DBTC)-induced pancreatitis. A further aim was to examine whether hyperthermia induces changes in transforming growth factor-beta1 (TGF-beta1). Following pretreatment without or with a single or double hyperthermia, pancreatitis was induced by application of cerulein or DBTC. Pancreatic HSP and TGF-beta1 expression were studied by immunoblotting. Pancreas injury was assessed by light microscopy and serum pancreatic enzyme activity. Hyperthermia as well as DBTC induced HSP72, whereas cerulein did not. A double hyperthermia led to a further increase in HSP72 compared to a single heat stress. In both models, hyperthermia significantly reduced pancreatic injury. Although a double hyperthermia slightly decreased the severity of cerulein pancreatitis compared to a single heat treatment, an improved pancreas protection against DBTC cytotoxicity was not achieved. We also found that hyperthermia induces the expression of TGF-beta1. In conclusion, hyperthermia preconditioning exerts protective effects against two pathophysiologically different types of pancreatitis by a mechanism that involves the up-regulation of HSP70 isoforms as well as TGF-beta1.
authors.affiliation: Institute of Clinical Chemistry, University of Rostock, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Weber
authors.forename: A C
authors.initials: AC
authors.lastname: Wagner
authors.forename: L
authors.initials: L
authors.lastname: Jonas
authors.forename: J
authors.initials: J
authors.lastname: Merkord
authors.forename: T
authors.initials: T
authors.lastname: Höfken
authors.forename: H
authors.initials: H
authors.lastname: Nizze
authors.forename: P
authors.initials: P
authors.lastname: Leitzmann
authors.forename: B
authors.initials: B
authors.lastname: Göke
authors.forename: P
authors.initials: P
authors.lastname: Schuff-Werner
mesh_terms: D000818:Animals; D002108:Ceruletide; D005260:Female; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006979:Hyperthermia, Induced; D009947:Organotin Compounds; D010179:Pancreas / Q000473:pathology; D019283:Pancreatitis, Acute Necrotizing / Q000139:chemically induced / Q000473:pathology*; D051381:Rats; D011917:Rats, Inbred Lew; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D009947:Organotin Compounds; D016212:Transforming Growth Factor beta; D002108:Ceruletide; C020735:dibutyldichlorotin