A simple and rapid method for purifying staphylococcal exfoliative toxin A.
A rapid and efficient method of purification of staphylococcal exfoliative toxin A, the causative agent of staphylococcal scalded skin syndrome (SSSS), has been developed. It is based on ammonium sulfate precipitation of the culture supernatant and hydrophobic interaction chromatography on Phenyl-Sepharose CL-4B. This procedure results in 87-fold purification of this toxin, which appears as a single band in sodium dodecyl sulfate-polyacrylamide gels.
Yamazaki
T
T
Department of Molecular Genetics, Kohno Clinical Medicine Research Institute, Tokyo, Japan.
Sakurai
S
S
Matsuo
N
N
Machida
K
K
Kohno
M
M
D000645:Ammonium Sulfate; D000818:Animals; D002021:Buffers; D004591:Electrophoresis, Polyacrylamide Gel; D004768:Enterotoxins / Q000302:isolation & purification*; D005083:Exfoliatins / Q000302:isolation & purification*; D017889:Exfoliation Syndrome / Q000209:etiology; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008813:Mice, Inbred ICR; D010710:Phosphates; D017680:Potassium Compounds; D012685:Sepharose / Q000031:analogs & derivatives; D013211:Staphylococcus aureus; D013997:Time Factors
D002021:Buffers; D004768:Enterotoxins; D005083:Exfoliatins; D010710:Phosphates; D017680:Potassium Compounds; C026066:enterotoxin A, Staphylococcal; C020927:phenyl-sepharose; D012685:Sepharose; C013216:potassium phosphate; C018279:sodium phosphate; D000645:Ammonium Sulfate