Fifty microsatellite markers for Japanese quail.
A Japanese quail genomic library enriched for (CA/GT)n simple sequence repeats was screened and positive clones were sequenced. Fifty original microsatellite sequences were isolated that consisted mainly of perfect repeats of the dinucleotide (CA/GT)n motif and a corresponding number of polymerase chain reaction (PCR) primer pairs complementary to unique DNA sequences flanking the microsatellite repeats were designed to detect the repeats. Forty-six percent (23 of 50) of the markers revealed polymorphism in two unrelated quail individuals (one male and one female) randomly sampled from a population of wild quail origin. All 50 primer pairs were tested in the PCR for their ability to amplify chicken genomic DNA. Amplification products were obtained for 14 (28.0%) of the markers at the annealing temperature optimized for quail. These results provide an opportunity to begin characterizing the quail genome for the development of a genetic map for this economically valuable species and the eventual construction of a comparative genetic map in Phasianidae, which comprises a number of agriculturally important species of poultry.
Kayang
B B
BB
United Graduate School of Agricultural Science, Gifu University, Japan.
Inoue-Murayama
M
M
Nomura
A
A
Kimura
K
K
Takahashi
H
H
Mizutani
M
M
Ito
S
S
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003370:Coturnix / Q000235:genetics*; D017931:DNA Primers; D018076:DNA, Complementary; D018900:Dinucleotide Repeats; D005260:Female; D015636:Magnesium Chloride; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods
D017931:DNA Primers; D018076:DNA, Complementary; D015636:Magnesium Chloride