PCR-based identification of Bacillus thuringiensis isolated from soil samples in Nigeria.
Six isolates of Bacillus thuringiensis isolated from soil samples confirmed to be toxic to mosquito larvae were differentiated using a PCR-Based technique. Three of these isolates initially identified using a serological technique were further differentiated with the PCR amplification of the delta-endotoxin target sequences. Using the total DNA of isolates as template, at least four isolates yielded amplicons one or all the crystal protein genes, cryI a, b, c, or II with sizes ranging from 238-1070 bp. None of these isolates yielded an amplicon for any of Cry IV A, B and D tested. Of the four isolates identified by PCR technique one isolate remained unidentified by serology.
Ogunjimi
A A
AA
Department of Biochemistry, College of Medicine of the University of Lagos, Nigeria.
Gbenle
G O
GO
Olukoya
D K
DK
Akinrimisi
E O
EO
D000818:Animals; D001413:Bacillus thuringiensis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins; D009033:Culicidae; D017931:DNA Primers; D004731:Endotoxins / Q000235:genetics; D006460:Hemolysin Proteins; D007814:Larva; D009549:Nigeria; D010572:Pest Control, Biological; D016133:Polymerase Chain Reaction / Q000379:methods; D012988:Soil Microbiology
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D017931:DNA Primers; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis