Antibodies to N-hydroxy-2-aminofluorene modified DNA as probes in the study of DNA reacted with derivatives of 2-acetylaminofluorene.
Antibodies were elicited in rabbits immunized with a mixture of methylated bovine serum albumin and N-hydroxy-2-aminofluorene reacted DNA (DNA-AF). These antibodies were used in competition radioimmunoassays (RIA) with [3H]DNA-AF as radioactive antigen and different N-acetoxy-2-acetylaminofluorene (N-AcO-AAF) and N-hydroxy-2aminofluorene (N-OH-AF) reacted DNAs, deoxyguanosine, dGMP, dGpA and dApG as inhibitors. Based on the results of RIA it is concluded that the binding sites of the two residues, -AF and -AAF, to guanosine in DNA, are essentially the same. Moreover, the same sites seem to be reactive also in the direct reaction of N-AcO-AAF with dGMP. Circular dichroism measurements of modified DNA confirm the RIA results.
Spodheim-Maurizot
M
M
Centre de Biophysique Moléculaire, CNRS, Orléans, France.
Saint-Ruf
G
G
Leng
M
M
D000099:Acetoxyacetylaminofluorene / Q000378:metabolism* / Q000633:toxicity; D000477:Alkylating Agents / Q000378:metabolism* / Q000633:toxicity; D000818:Animals; D000906:Antibodies; D001667:Binding, Competitive; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000187:drug effects / Q000378:metabolism*; D018736:DNA Adducts / Q000276:immunology* / Q000378:metabolism; D003848:Deoxyguanine Nucleotides / Q000378:metabolism / Q000494:pharmacology; D005449:Fluorenes / Q000276:immunology* / Q000378:metabolism* / Q000633:toxicity; D011233:Precipitin Tests; D011817:Rabbits; D011863:Radioimmunoassay
C052668:2-aminofluorene-DNA complex; D000477:Alkylating Agents; D000906:Antibodies; D018736:DNA Adducts; D003848:Deoxyguanine Nucleotides; D005449:Fluorenes; D000099:Acetoxyacetylaminofluorene; C001368:N-hydroxy-2-aminofluorene; D004247:DNA