title: Physicochemical characterisation of the pertussis vaccine.
abstract: The characterisation of an acellular pertussis vaccine composed of a genetically modified pertussis toxin, filamentous haemagglutinin and pertactin is described. The three antigens are submitted to a mild treatment with formaldehyde in the presence of lysine before their use in vaccine formulation. Characterisation is performed by amino acid analysis, SDS-PAGE, analytical size exclusion chromatography and, in the case of pertactin, isoelectrofocusing. The effect of some variables on pertactin formaldehyde treatment has been studied by means of isoelectrofocusing and mouse immunogenicity.
authors.affiliation: Chiron Vaccines, Siena, Italy
authors.forename: C
authors.initials: C
authors.lastname: Ceccarini
authors.forename: M
authors.initials: M
authors.lastname: Contorni
authors.forename: P
authors.initials: P
authors.lastname: Costantino
authors.forename: S
authors.initials: S
authors.lastname: D'Ascenzi
authors.forename: E
authors.initials: E
authors.lastname: Gallo
authors.forename: M
authors.initials: M
authors.lastname: Maffei
authors.forename: D
authors.initials: D
authors.lastname: Mannucci
authors.forename: I
authors.initials: I
authors.lastname: Marsili
authors.forename: C
authors.initials: C
authors.lastname: Magagnoli
authors.forename: S
authors.initials: S
authors.lastname: Peppoloni
authors.forename: R
authors.initials: R
authors.lastname: Rappuoli
authors.forename: N
authors.initials: N
authors.lastname: Ravenscroft
authors.forename: S
authors.initials: S
authors.lastname: Ricci
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D015153:Blotting, Western; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005557:Formaldehyde / Q000737:chemistry; D007525:Isoelectric Focusing; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D037342:Pertussis Toxin; D010567:Pertussis Vaccine / Q000737:chemistry* / Q000276:immunology; D010566:Virulence Factors, Bordetella / Q000737:chemistry / Q000235:genetics / Q000276:immunology
chemical_list: D000596:Amino Acids; D010567:Pertussis Vaccine; D010566:Virulence Factors, Bordetella; D005557:Formaldehyde; D037342:Pertussis Toxin