Fine-mapping and construction of a bovine contig spanning the ovine callipyge locus.
The callipyge (CLPG) gene was fine-mapped by linkage analysis to a 4.6-cM chromosome interval on distal ovine OAR18q, flanked by microsatellite markers IDVGA30 and OY3. The OAR18q linkage map and human HSA14q transcript map were aligned by genotyping two bovine-hamster whole-genome radiation hybrid panels with the microsatellite markers, as well as with sequences corresponding to HSA 14q genes. Using Type I loci mapping to the IDVGA30-OY3 interval as anchor points, we have constructed a 1.4-Mb bovine BAC contig containing the IDVGA30-OY3 interval. We demonstrate that the IDVGA30-OY3 interval spans approximately 770 kb and contains at least four genes: YY1, WARS, DLK1, and GTL2.
Shay
T L
TL
Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan 84322-4700, USA.
Berghmans
S
S
Segers
K
K
Meyers
S
S
Beever
J E
JE
Womack
J E
JE
Georges
M
M
Charlier
C
C
Cockett
N E
NE
D000818:Animals; D002417:Cattle; D003001:Cloning, Molecular; D020451:Contig Mapping; D006224:Cricetinae; D003433:Crosses, Genetic; D005838:Genotype; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D024510:Muscle Development; D018656:Muscle Fibers, Fast-Twitch / Q000378:metabolism; D018482:Muscle, Skeletal / Q000254:growth & development / Q000378:metabolism; D011506:Proteins / Q000235:genetics*; D021101:Radiation Hybrid Mapping; D012756:Sheep
D011506:Proteins