Growth and recombination of phage lambda in the presence of exonuclease V from Bacillus subtilis.
When expressed in Escherichia coli, the AddAB exonuclease/recombinase from Bacillus subtilis blocks the growth of phage lambda. Mutants of lambda that are deleted for ea47, a gene of unknown function which is expressed early in the lytic cycle, are not blocked for growth. The blocked-growth phenotype of lambda ea47+ in the presence of AddAB is expressed only when phage DNA replication is permitted.
Stahl
F
F
Institute of Molecular Biology, University of Oregon, Eugene 97403-1229, USA. fstahl@morel.uoregon.edu
Bowers
R
R
Mooney
D
D
Myers
R
R
Stahl
M
M
Thomason
L
L
D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics; D010582:Bacteriophage lambda / Q000235:genetics* / Q000502:physiology*; D004247:DNA / Q000378:metabolism; D004261:DNA Replication; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D043211:Exodeoxyribonuclease V; D005090:Exodeoxyribonucleases / Q000235:genetics* / Q000502:physiology*; D017353:Gene Deletion; D009154:Mutation; D010641:Phenotype; D010957:Plasmids / Q000378:metabolism; D011938:Rec A Recombinases / Q000235:genetics; D011995:Recombination, Genetic; D013997:Time Factors
D004247:DNA; D011938:Rec A Recombinases; D005090:Exodeoxyribonucleases; C407424:AddAB enzyme; D043211:Exodeoxyribonuclease V