Inhibition of formation of protease-resistant prion protein by Trypan Blue, Sirius Red and other Congo Red analogs.
Five compounds related to Congo Red were found to inhibit generation of protease-resistant prion protein in a cell-free system. In this assay Trypan Blue, Evans Blue, Sirius Red F3B, Primuline and Thioflavin-S were all more inhibitory than Congo Red itself. In scrapie-infected mouse neuroblastoma cells one compound, Sirius Red F3B, was capable of blocking the formation of protease-resistant prion protein to a similar extent as Congo Red; however, the other four compounds were less effective. Some of these compounds should be considered for testing in TSE disease models in live animals.
Demaimay
R
R
RML, NIAID, NIH, Hamilton, Montana 59840, USA.
Chesebro
B
B
Caughey
B
B
D000818:Animals; D001391:Azo Compounds / Q000494:pharmacology; D015153:Blotting, Western; D002474:Cell-Free System; D003224:Congo Red / Q000031:analogs & derivatives* / Q000494:pharmacology*; D006224:Cricetinae; D051379:Mice; D009447:Neuroblastoma; D009474:Neurons / Q000737:chemistry; D018621:PrPC Proteins / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D018620:PrPSc Proteins / Q000737:chemistry* / Q000187:drug effects* / Q000378:metabolism; D012608:Scrapie / Q000378:metabolism; D013844:Thiazoles / Q000494:pharmacology; D014343:Trypan Blue / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D001391:Azo Compounds; D018621:PrPC Proteins; D018620:PrPSc Proteins; D013844:Thiazoles; C009798:C.I. direct red 80; D003224:Congo Red; C035646:primuline; D014343:Trypan Blue