Quantitative determination of L-775,606, a selective 5-hydroxytryptamine 1D agonist, in rat plasma using automated sample preparation and detection by liquid chromatography-tandem mass spectrometry.
L-775,606 is under investigation as a selective 5-hydroxytryptamine 1D agonist for the treatment of migraine. A reliable and sensitive method for the analysis of L-775,606 in plasma was required in order to support preclinical evaluation of this compound. A semi-automated sample preparation method using the Beckman Biomek 2000 workstation to perform all liquid handling tasks has been established. The sample analysis was performed using HPLC-MS-MS with a cycle time of 3.5 min per sample. Intra- and inter-day assay accuracy and precision are excellent with a calibration range of 1-2000 ng/ml and a reproducible limit of quantification of 1 ng/ml.
Locker
K L
KL
Department of Medicinal Chemistry (Drug Metabolism and Pharmacokinetics Section), Merck Sharp and Dohme Research Laboratories, Essex, UK. karen_locker@merck.com
Morrison
D
D
Watt
A P
AP
D000818:Animals; D001331:Automation; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D007211:Indoles / Q000097:blood*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D010879:Piperazines / Q000097:blood*; D051381:Rats; D017207:Rats, Sprague-Dawley; D044346:Receptor, Serotonin, 5-HT1D; D011985:Receptors, Serotonin / Q000187:drug effects; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017366:Serotonin Receptor Agonists / Q000097:blood*
C117663:1-(3-(5-(1,2,4-triazol-4-yl)-1H-indol-3-yl)propyl)-4-(2-(3-fluorophenyl)ethyl)piperazine; D007211:Indoles; D010879:Piperazines; D044346:Receptor, Serotonin, 5-HT1D; D011985:Receptors, Serotonin; D017366:Serotonin Receptor Agonists