title: Preparative isolation and purification of lutein from the microalga chlorella vulgaris by high-speed counter-current chromatography.
abstract: High-speed counter-current chromatography (HSCCC) was applied to the isolation and purification of lutein from microalgae. Analytical HSCCC was used for the preliminary selection of a suitable solvent system composed of n-hexane-ethanol-water (4:3:1, v/v). Using the above solvent system, preparative HSCCC was successfully performed yielding lutein at 98% purity from 200 mg of the crude extract in a one-step separation.
authors.affiliation: Department of Botany, The University of Hong Kong, China.
authors.forename: H B
authors.initials: HB
authors.lastname: Li
authors.forename: F
authors.initials: F
authors.lastname: Chen
authors.forename: T Y
authors.initials: TY
authors.lastname: Zhang
authors.forename: F Q
authors.initials: FQ
authors.lastname: Yang
authors.forename: G Q
authors.initials: GQ
authors.lastname: Xu
mesh_terms: D002708:Chlorella / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D014975:Lutein / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D014975:Lutein