title: Intratesticular transplantation of testicular cells from leukemic rats causes transmission of leukemia.
abstract: A rat T-cell leukemia model was used to study the safety of germ cell transplantation as a mean of preventing infertility in males undergoing gonadotoxic cancer treatment. Donor germ cells were harvested from the testes of terminally ill leukemic rats and were either used directly or cryopreserved and thawed before transplantation by rete testis microinjection. All rats transplanted with testicular cells from leukemic donors developed signs of terminal rat T-cell leukemia, whereas control animals remained healthy. Cryopreservation of the donor germ cells caused a 3- to 6-day delay in the terminal phase of leukemia. When a known number of leukemic cells were mixed with germ cells and microinjected into the testis, the rate of appearance of terminal leukemia was directly related to the number of transferred leukemic lymphoblasts. As few as 20 leukemic cells were able to cause a cancer relapse resulting in terminal leukemia 21 days after transplantation in three of five transplanted animals. Our results demonstrate that germ cell transplantation with the presently used techniques is not safe enough for clinical use. Improved methods for purging testicular specimens of cancer cells or totally new approaches with transient xenogenetic host models to detect contamination of malignant cells must be developed before this technique can be offered to patients without fear of disease relapse.
authors.affiliation: Pediatric Endocrinology Unit, Karolinska Institute, Karolinska Hospital, Stockholm, Sweden. kirsi.Jahnukainen@hus.fi
authors.forename: K
authors.initials: K
authors.lastname: Jahnukainen
authors.forename: M
authors.initials: M
authors.lastname: Hou
authors.forename: C
authors.initials: C
authors.lastname: Petersen
authors.forename: B
authors.initials: B
authors.lastname: Setchell
authors.forename: O
authors.initials: O
authors.lastname: Söder
mesh_terms: D000818:Animals; D017690:Cell Transplantation / Q000009:adverse effects; D015925:Cryopreservation; D015458:Leukemia, T-Cell / Q000209:etiology / Q000401:mortality / Q000473:pathology*; D008297:Male; D051381:Rats; D013093:Spermatogonia / Q000166:cytology / Q000637:transplantation; D013094:Spermatozoa / Q000166:cytology / Q000637:transplantation; D015996:Survival Rate; D013737:Testis / Q000166:cytology / Q000637:transplantation*