title: Activity of artichoke leaf extract on reactive oxygen species in human leukocytes.
abstract: Artichoke leaf extract was studied in human leukocytes for activity against oxidative stress using flow cytometry and dichlorofluorescin diacetate as a fluorescence probe. It produces a concentration-dependent inhibition of oxidative stress when cells are stimulated with agents that generate reactive oxygen species (ROS): hydrogen peroxide, phorbol-12-myristate-13-acetate (PMA), and N-formyl-methionyl-leucyl-phenylalanine (FMLP). Cynarin, caffeic acid, chlorogenic acid, and luteolin, constituents of artichoke leaf extract, also show a concentration-dependent inhibitory activity in the above models, contributing to the antioxidant activity of the extract in human neutrophils.
authors.affiliation: Untitat de Farmacologia i Farmacognòsia, Facultat de Farmàcia, Universitat de Barcelona, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Pérez-García
authors.forename: T
authors.initials: T
authors.lastname: Adzet
authors.forename: S
authors.initials: S
authors.lastname: Cañigueral
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D019659:Asteraceae; D002109:Caffeic Acids / Q000494:pharmacology; D002726:Chlorogenic Acid / Q000494:pharmacology; D002934:Cinnamates / Q000494:pharmacology; D005419:Flavonoids / Q000494:pharmacology; D005434:Flow Cytometry; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D066298:In Vitro Techniques; D047311:Luteolin; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D018375:Neutrophil Activation; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate
chemical_list: D000975:Antioxidants; D002109:Caffeic Acids; D002934:Cinnamates; D005419:Flavonoids; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D002726:Chlorogenic Acid; D009240:N-Formylmethionine Leucyl-Phenylalanine; C100257:cynarine; D006861:Hydrogen Peroxide; D047311:Luteolin; D013755:Tetradecanoylphorbol Acetate; C040048:caffeic acid