title: Effect of rotavirus infection on lipid composition and glucose uptake in infant mouse intestine.
abstract: OBJECTIVE
  To study the effect of rotavirus infection on lipid composition and glucose uptake in small intestine of infant mice.
  
  
  METHODS
  Thirty six 7-day old balb/c mice were given 50 mL (100 ID50) rotavirus suspension orally; 36 control animals received only normal saline. Body weight of animals was recorded. Six animals in each group were sacrificed on days 0, 1, 3, 5, 7 and 10 post-inoculation (pi). Intestines were removed, everted and homogenized in ice-cold saline. Cholesterol and phospholipid content, glucose uptake and alkaline phosphatase in the jejunum and ileum were determined.
  
  
  RESULTS
  Rotavirus infection led to a significant decrease in body weight with on days 3 and 5 pi as compared to controls, and to an increase in cholesterol and phospholipid content of the intestine. Uptake of glucose also increased significantly. Alkaline phosphatase activity was significantly reduced on days 3 and 5 pi as compared to controls.
  
  
  CONCLUSIONS
  This study provides characterization of the lipid composition and uptake of glucose in infant mice small intestinal segments during rotavirus infection.
authors.affiliation: Department of Gastroenterology, Postgraduate Institute of Medical Education and Research, Chandigarh.
authors.forename: R
authors.initials: R
authors.lastname: Katyal
authors.forename: S
authors.initials: S
authors.lastname: Rana
authors.forename: K
authors.initials: K
authors.lastname: Vaiphei
authors.forename: S
authors.initials: S
authors.lastname: Ojha
authors.forename: V
authors.initials: V
authors.lastname: Singh
authors.forename: K
authors.initials: K
authors.lastname: Singh
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D004195:Disease Models, Animal; D005260:Female; D005947:Glucose / Q000378:metabolism*; D007408:Intestinal Absorption / Q000502:physiology*; D007583:Jejunum / Q000378:metabolism* / Q000821:virology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010743:Phospholipids / Q000378:metabolism*; D011336:Probability; D012016:Reference Values; D012400:Rotavirus Infections / Q000378:metabolism*
chemical_list: D010743:Phospholipids; D005947:Glucose