Polymorphism of the D12S66 system in the Japanese population and its detection using degraded DNA.
The short tandem repeat (STR) polymorphism of the locus D12S66 was amplified by PCR and analyzed by denaturing polyacrylamide gel electrophoresis followed by silver staining. Among 190 DNA samples from the Japanese population, six alleles were observed. The genotypic distribution meets Hardy-Weinberg expectations, and the heterozygosity was 52.7%. When sequences of the allelic products were compared, each allelic segment was 153-173 bp in size, and contained 9 to 14 GATA tetranucleotide repeat motifs. Amplification of the locus using 27 tooth and blood stain samples as sources of degraded DNA resulted in low backgrounds and reproducible patterns, suggesting the usefulness of the application of this locus for material examination.
Minaguchi
K
K
Department of Forensic Odontology, Tokyo Dental College, 1-2-2 Masago, Mihama-ku, Chiba 261-8502, Japan.
Haga
T
T
D000225:Adenine; D000483:Alleles; D020029:Base Pairing; D001769:Blood; D002874:Chromosome Mapping; D004396:Coloring Agents; D004247:DNA / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D006580:Genetic Carrier Screening; D005838:Genotype; D006147:Guanine; D006801:Humans; D007564:Japan; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D015203:Reproducibility of Results; D012835:Silver Nitrate; D020080:Tandem Repeat Sequences / Q000235:genetics; D013941:Thymine; D014070:Tooth / Q000378:metabolism
D004396:Coloring Agents; D006147:Guanine; D004247:DNA; D012835:Silver Nitrate; D000225:Adenine; D013941:Thymine