Antinociceptive pattern of flavone and its mechanism as tested by formalin assay.
Flavone, dextrose and long swim stress exhibited antinociception. Degree of antinociception was greater with long swim stress as compared to flavone or dextrose. Combination of these treatments resulted in potentiation of antinociception. Naloxone (opioid antagonist; 5 mg/kg i.p.) antagonised flavone or long stress induced antinociception showing opioid medicated mechanism, however, failed to reverse the potentiated antinociceptive component recorded in long stressed animals which received flavone and dextrose. Antinociceptive activity of flavone, dextrose and long swim stress which was documented by acetic acid assay has been confirmed in the present study. Role for opioid system in this action has been demonstrated. Therefore, formalin test can also be considered as an useful assay procedure for testing flavonoids. However, like acetic acid assay this assay procedure also has the limitation that it is unable to detect minor changes in the degree of antinociception produced by physiological interventions such as long swim and dextrose.
Rajendran
N N
NN
Medicinal Chemistry Research Centre, Madras Medical College and Research Institute, Chennai 600 003, India.
Thirugnanasambandam
P
P
Viswanathan
S
S
Parvathavarthini
S
S
Ramaswamy
S
S
D000700:Analgesics / Q000494:pharmacology*; D000818:Animals; D047309:Flavones; D005419:Flavonoids / Q000494:pharmacology*; D005557:Formaldehyde; D005947:Glucose / Q000494:pharmacology; D008297:Male; D051379:Mice; D018847:Opioid Peptides / Q000502:physiology; D010146:Pain / Q000188:drug therapy / Q000503:physiopathology; D013312:Stress, Physiological / Q000503:physiopathology; D013550:Swimming
D000700:Analgesics; D047309:Flavones; D005419:Flavonoids; D018847:Opioid Peptides; D005557:Formaldehyde; D005947:Glucose; C043562:flavone