title: The influence of storage solution on dentin bond durability of resin cement.
abstract: OBJECTIVE
  This study was conducted to determine the influence of storage solution on the bond durability of three resin cements to bovine dentin over the period of 1 year.
  
  
  METHODS
  Ten bovine dentin specimens were tested for shear bond strength with each material (Panavia 21, Kuraray Co.; BISTITE, Tokuyama Co; MASA Bond, Sun Medical Co.) and storage mode, listed below. Four storage environments were studied as follows: water changed every day for 1 year; water unchanged for 1 year; Phosphate Buffered Saline (PBS) changed every week over 1 year; PBS unchanged for 1 year. Ten teeth were also tested for each material at 1 day as a control. The mode of failure was classified after fracture of the bonds by SEM observation. The means of the bond strengths were compared statistically by two-way ANOVA and Fisher's PLSD test (p < 0.05). Results for the mode of fracture were analyzed using the Mann-Whitney U test.
  
  
  RESULTS
  Although there was no statistical difference in the mean bond strengths between the water and PBS storage solutions (p > 0.05) in all cements, the results for the shear bond strengths in the changed storage solution groups were significantly lower than those where the storage solution remained unchanged (p < 0.05). There were statistical differences between the 1 day results and the changed water groups among all cements (p < 0.05).
  
  
  CONCLUSIONS
  The storage condition influenced the long-term durability of dentin bonding with resin cements.
authors.affiliation: Department of Operative Dentistry, Faculty of Dentistry, Tokyo Medical and Dental University, Tokyo, Japan. y.kitasako.odnt@dent.tmd.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Kitasako
authors.forename: M F
authors.initials: MF
authors.lastname: Burrow
authors.forename: T
authors.initials: T
authors.lastname: Nikaido
authors.forename: J
authors.initials: J
authors.lastname: Tagami
mesh_terms: D000268:Adhesiveness; D000704:Analysis of Variance; D000818:Animals; D002417:Cattle; D001840:Dental Bonding; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D004356:Drug Storage; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D019279:Resin Cements / Q000737:chemistry*; D012965:Sodium Chloride; D012996:Solutions / Q000737:chemistry; D018709:Statistics, Nonparametric; D013718:Tensile Strength; D014867:Water
chemical_list: D017220:Dentin-Bonding Agents; D019279:Resin Cements; D012996:Solutions; D014867:Water; D012965:Sodium Chloride