Identification and characterization of a novel gene, hos2+, the function of which is necessary for growth under high osmotic stress in fission yeast.
hos2 mutants of the fission yeast Schizosaccharomyces pombe showed the phenotype of high osmolarity sensitivity for growth. An S. pombe strain carrying the hos2-M10 allele cannot form colonies on agar plates containing 2 M glucose, but the parental strain can do so very well, as demonstrated previously. In this study, the hos2+ gene was identified as one that encodes a small protein of 94 amino acids, which shows no sequence similarity to any other proteins in the current databases. The hos2-M10 mutation resulted in Gln-62 to TAG-termination codon. A Hos2-defective (hos2delta) strain, which we then constructed, showed the phenotype of high osmolarity sensitivity, as in the case of the original hos2-M10 mutant. For this hos2delta mutant, three multicopy suppressor genes were isolated and one of which was identified as the pgk1+ gene, encoding a phosphoglycerate kinase.
Nakamichi
N
N
Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Japan.
Yamamoto
E
E
Yamada
H
H
Aiba
H
H
Mizuno
T
T
D000595:Amino Acid Sequence; D018076:DNA, Complementary / Q000032:analysis; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009997:Osmotic Pressure; D012568:Schizosaccharomyces / Q000235:genetics* / Q000254:growth & development; D029702:Schizosaccharomyces pombe Proteins; D014882:Water-Electrolyte Balance / Q000235:genetics
D018076:DNA, Complementary; D005656:Fungal Proteins; C421189:Hos2 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins