title: Modeling the growth boundary of Staphylococcus aureus for risk assessment purposes.
abstract: Knowing the precise boundary for growth of Staphylococcus aureus is critical for food safety risk assessment, especially in the formulation of safe, shelf-stable foods with intermediate relative humidity (RH) values. To date, most studies and resulting models have led to the presumption that S. aureus is osmotolerant. However, most studies and resulting models have focused on growth kinetics using NaCl as the humectant. In this study, glycerol was used to investigate the effects of a glass-forming nonionic humectant to avoid specific metabolic aspects of membrane ion transport. The experiments were designed to produce a growth boundary model as a tool for risk assessment. The statistical effects and interactions of RH (84 to 95% adjusted by glycerol), initial pH (4.5 to 7.0 adjusted by HC1), and potassium sorbate (0, 500, or 1,000 ppm) or calcium propionate (0, 500, or 1,000 ppm) on the aerobic growth of a five-strain S. aureus cocktail in brain heart infusion broth were explored. Inoculated broths were distributed into microtiter plates and incubated at 37 degrees C over appropriate saturated salt slurries to maintain RH. Growth was monitored by turbidity during a 24-week period. Toxin production was explored by enterotoxin assay. The 1,280 generated data points were analyzed by SAS LIFEREG procedures, which showed all studied parameters significantly affected the growth responses of S. aureus with interactions between RH and pH. The resulting growth/no growth boundary is presented.
authors.affiliation: Nabisco, Inc., East Hanover, New Jersey 07936, USA. stewartc@nabisco.com
authors.forename: C M
authors.initials: CM
authors.lastname: Stewart
authors.forename: M B
authors.initials: MB
authors.lastname: Cole
authors.forename: J D
authors.initials: JD
authors.lastname: Legan
authors.forename: L
authors.initials: L
authors.lastname: Slade
authors.forename: M H
authors.initials: MH
authors.lastname: Vandeven
authors.forename: D W
authors.initials: DW
authors.lastname: Schaffner
mesh_terms: D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D003470:Culture Media / Q000737:chemistry; D005516:Food Microbiology; D005990:Glycerol / Q000494:pharmacology*; D006813:Humidity; D006863:Hydrogen-Ion Concentration; D008954:Models, Biological; D011422:Propionates / Q000494:pharmacology*; D018570:Risk Assessment; D013011:Sorbic Acid / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development*
chemical_list: D003470:Culture Media; D011422:Propionates; C514136:calcium propionate; C029658:propionic acid; D005990:Glycerol; D013011:Sorbic Acid