title: DNA methylation contributes to tissue- and allele-specific expression of the T-cell differentiation marker RT6.
abstract: We investigated the role of DNA methylation in gene regulation of the rat T-cell differentiation marker RT6. Analysis of the methylation status of various tissues revealed that the RT6 promoter was hypomethylated in RT6-expressing tissues, and methylated in nonexpressing ones. Remarkably, among RT6-nonexpressing tissues, the extent of methylated regions varied greatly between lymphatic tissues, where regions larger than 23 kb were methylated, and nonlymphatic tissues, where methylation was restricted to a 3- to 4-kb region surrounding the promoter. We have previously shown that cis-regulatory elements determine differential expression of the two RT6 alleles in a subpopulation of T cells. We now show that the RT6 alleles in these cells differed in their methylation status. The promoter region of the silent allele was methylated, while that of the transcribed allele was not. Upon treatment of RT6-nonexpressing thymoma cells with the methyltransferase inhibitor 5-azacytidine, RT6 expression was induced. In RT6 heterozygous hybridoma cells, expressing only one RT6 allele, induction of the silent, methylated RT6 allele was observed. Sensitivity of the RT6 promoter to DNA methylation was demonstrated by promoter-specific in vitro methylation, which inhibited RT6 promoter activity, while that of the SV40 promoter was not influenced. Our findings indicate that DNA methylation plays an important role in the control of monoallelic and tissue-specific RT6 expression.
authors.affiliation: Institute for Immunology, University Hospital Eppendorf, Hamburg, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Rothenburg
authors.forename: F
authors.initials: F
authors.lastname: Koch-Nolte
authors.forename: H G
authors.initials: HG
authors.lastname: Thiele
authors.forename: F
authors.initials: F
authors.lastname: Haag
mesh_terms: D036002:ADP Ribose Transferases / Q000235:genetics*; D000483:Alleles; D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics*; D001374:Azacitidine / Q000494:pharmacology; D001402:B-Lymphocytes / Q000378:metabolism; D002478:Cells, Cultured; D019175:DNA Methylation; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000187:drug effects; D020868:Gene Silencing / Q000187:drug effects; D008562:Membrane Glycoproteins / Q000235:genetics*; D009928:Organ Specificity; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015533:Transcriptional Activation / Q000187:drug effects; D014162:Transfection
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D036002:ADP Ribose Transferases; C090163:Art2b protein, rat; D001374:Azacitidine