Evidence for the binding of a human sperm component with diaphanous protein.
The YWK-II component of human sperm membrane is related to the betaA4-amyloid precursor protein (APP) of Alzheimer's disease. A yeast 2-hybrid system was used to screen a mouse testis cDNA expression library for potential ligands capable of interacting with the extracellular domain of the YWK-II component. One of the bound proteins was identified as hDIA1, which has 96% identity with p140mDia. These proteins are members of the formin homology family and participate in cytokinesis and organization of the actin cytoskeleton. By interacting with these diaphanous proteins, the YWK-II component may be involved in germ cell differentiation and in the structural formation of the acrosome.
Zhang
S M
SM
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China. wanglf@cdm.imicams.ac.cn
Miao
S Y
SY
Wang
L F
LF
Koide
S S
SS
D000595:Amino Acid Sequence; D016564:Amyloid beta-Protein Precursor; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D015723:Gene Library; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D011485:Protein Binding; D012441:Saccharomyces cerevisiae; D017386:Sequence Homology, Amino Acid; D013094:Spermatozoa / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
C489697:APLP1 protein, human; C084104:APLP2 protein, human; D016564:Amyloid beta-Protein Precursor; C078201:Aplp1 protein, mouse; C492952:Aplp2 protein, mouse; D002352:Carrier Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins