title: Different classes of retrotransposons in coniferous spruce species.
abstract: We have used the conservation of reverse transcriptase and integrase domains among retroelements to PCR-amplify three well-known types of these mobile genetic elements. Reverse transcriptase sequences from Ty1-copia were identified in spruce in this way, as well as integrase sequences from the Ty3-gypsy group. Using these sequences as probes against a Picea glauca genomic bank, individual members from the LTR (long terminal direct repeat) groups were obtained. A partial Ty1-copia-type element named Spcl was isolated along with a Ty3-gypsy-type element named Spdl. Genomic Southern hybridizations revealed the complexity and high copy number of LTR retrotransposons in black and white spruce.
authors.affiliation: Centre de recherche en biologie forestière, Université Laval, Sainte-Foy, QC, Canada.
authors.forename: Y
authors.initials: Y
authors.lastname: L'Homme
authors.forename: A
authors.initials: A
authors.lastname: Séguin
authors.forename: F M
authors.initials: FM
authors.lastname: Tremblay
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D032462:Cycadopsida / Q000145:classification / Q000235:genetics*; D018744:DNA, Plant / Q000032:analysis; D014644:Genetic Variation; D018745:Genome, Plant; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D018626:Retroelements / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D020079:Terminal Repeat Sequences / Q000235:genetics
chemical_list: D018744:DNA, Plant; D018626:Retroelements