title: Human anti-DNA secretory immunglobulins A possess endonuclease activity and they are able to cause the destruction of nuclear chromatin in vitro.
abstract: sIgA possessing ability to hydrolyse plasmid DNA to linear forms was purified from human milk by sequential chromatography on protein A-sepharose, DEAE-Fractogel and DNA-cellulose. It was discovered that incubation of sIgA with nuclei of porcine embryo kidney cells permeabilized by Triton X-100 causes formation of electrophoretically mobile forms of nuclear nucleic acids and inhibition of phosphorylation of nuclear proteins. We suppose that sIgA possessing affinity to DNA and endonuclease activity can cause degradation of cell nuclear chromatin.
authors.affiliation: Division of Regulatory Cell Systems O.V. Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Lviv.Kit@biochem.Iviv.ua
authors.forename: YuYa
authors.initials: Y
authors.lastname: Kit
authors.forename: E E
authors.initials: EE
authors.lastname: Mitrofanova
authors.forename: O E
authors.initials: OE
authors.lastname: Shestova
authors.forename: E V
authors.initials: EV
authors.lastname: Kuligina
authors.forename: I V
authors.initials: IV
authors.lastname: Romannikova
authors.forename: V A
authors.initials: VA
authors.lastname: Richter
mesh_terms: D000974:Antibodies, Antinuclear / Q000276:immunology*; D002467:Cell Nucleus / Q000201:enzymology; D002843:Chromatin / Q000378:metabolism*; D002852:Chromatography, Ion Exchange; D004247:DNA / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology*; D066298:In Vitro Techniques; D008895:Milk, Human / Q000276:immunology; D011494:Protein Kinases / Q000378:metabolism
chemical_list: D000974:Antibodies, Antinuclear; D002843:Chromatin; D007070:Immunoglobulin A; D004247:DNA; D011494:Protein Kinases