Glucose sensor with improved haemocompatibilty.
A new biocompatible copolymer has been synthesised and used in an electrochemical enzyme-based glucose sensor. The copolymer incorporates three segments including a monomer with an electrically neutral phosphorylcholine head group that is able to reject protein adsorption and two segments that increase the affinity to polyurethane substrate. Peel and solution circulation tests showed that this material has high attachment to polyurethane. With the new copolymer as the outermost layer and the polyurethane as the diffusion-limiting membrane, the sensor showed extended linearity up to 50 mM glucose and stable output in bovine serum for 70 h. During in vivo tests, the sensor exhibited a steady current signal and a rapid transient response when the glucose concentration was raised. These results imply that the haemocompatibility of the glucose sensor coated with the new copolymer has been improved, which is crucial for a sensor used for clinical real-time monitoring. The material may also be suitable for application to other implantable devices.
Yang
Y
Y
Centre for Science and Technology in Medicine, Keele University, Staffordshire, UK. bea00@keele.ac.uk
Zhang
S F
SF
Kingston
M A
MA
Jones
G
G
Wright
G
G
Spencer
S A
SA
D000818:Animals; D001672:Biocompatible Materials; D015374:Biosensing Techniques; D001786:Blood Glucose / Q000032:analysis; D005947:Glucose / Q000032:analysis*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010713:Phosphatidylcholines; D011108:Polymers; D011140:Polyurethanes; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D013696:Temperature
D001672:Biocompatible Materials; D001786:Blood Glucose; D010713:Phosphatidylcholines; D011108:Polymers; D011140:Polyurethanes; D005947:Glucose