Deletion of the gene encoding H-FABP/MDGI has no overt effects in the mammary gland.
Heart fatty acid binding protein (H-FABP) is expressed abundantly in the mammary gland. A number of in vitro studies have shown that H-FABP is functionally indistinguishable from a factor isolated from this organ, termed mammary derived growth inhibitor (MDGI), which specifically inhibits the proliferation of mammary tissue. We have previously shown that over-expression of H-FABP/MDGI in the mammary gland of transgenic mice has no discernable effects on cell proliferation or differentiation. In this report we describe knockout mouse in which the H-FABP/MDGI gene has been specifically disrupted. The mice exhibit no overt phenotype in the mammary gland, and we conclude that this gene does not play a specific role in regulating the normal development or function of this tissue.
Clark
A J
AJ
Department of Gene Expression and Development, Roslin Institute, Midlothian, Scotland, UK. john.clark@bbsrc.ac.uk
Neil
C
C
Gusterson
B
B
McWhir
J
J
Binas
B
B
D000818:Animals; D002352:Carrier Proteins / Q000235:genetics*; D000074026:Fatty Acid Binding Protein 3; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D008321:Mammary Glands, Animal / Q000033:anatomy & histology / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D008892:Milk / Q000737:chemistry*; D008894:Milk Proteins / Q000032:analysis
D002352:Carrier Proteins; C493409:Fabp3 protein, mouse; D000074026:Fatty Acid Binding Protein 3; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D008894:Milk Proteins