Enhanced effect of sulfonylurea (SU) in copolymer comprising a sugar moiety and SU derivative as double ligands on insulin secretion from MIN6 cells.
Copolymers composed of sulfonylurea (SU) as an antagonist of ATP-sensitive K+ channel and sugar moieties as double ligands were synthesized. Insulin secretion from MIN6 cells (insulinoma cell line) in contact with the copolymers was evaluated. MIN6 cells attached to the poly(N-p-vinylbenzyl-D-maltonamide-co-SU) [P(VMA-co-SU)]-coated dishes were in the more aggregated form as compared to other polymer-coated surfaces. By introducing SU into the sugar bearing homopolymer, an enhanced effect on insulin secretion from MIN6 cells was observed due to the specific interaction between SU ligands and SU receptors on the beta-cell membrane. P(VMA-co-SU) composed of SU and non-reducing glucose moieties demonstrated enhanced insulin secretion from MIN6 cells and faster proliferation of MIN6 cells as compared to poly(N-p-vinylbenzyl-D-lactonamide-co-SU) [P(VLA-co-SU)] probably owing to the glucose transporters presence on the MIN6 cell membrane. Insulin secretion from MIN6 cells pretreated with diazoxide as an agonist of ATP-sensitive K+ channel was suppressed.
Park
K
K
Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
Goto
M
M
Takei
R
R
Maruyama
A
A
Kobayashi
K
K
Miyazaki
J
J
Cho
C
C
Akaike
T
T
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002241:Carbohydrates / Q000737:chemistry*; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003981:Diazoxide / Q000494:pharmacology; D005905:Glyburide / Q000737:chemistry; D007004:Hypoglycemic Agents / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007785:Lactose / Q000031:analogs & derivatives* / Q000737:chemistry; D008024:Ligands; D008320:Maltose / Q000138:chemical synthesis; D051379:Mice; D008858:Microscopy, Phase-Contrast; D008956:Models, Chemical; D011108:Polymers / Q000737:chemistry*; D011137:Polystyrenes / Q000138:chemical synthesis / Q000737:chemistry; D026902:Potassium Channel Blockers; D013453:Sulfonylurea Compounds / Q000494:pharmacology*; D013997:Time Factors; D014665:Vasodilator Agents / Q000494:pharmacology
D002241:Carbohydrates; D007004:Hypoglycemic Agents; D007328:Insulin; D008024:Ligands; D011108:Polymers; D011137:Polystyrenes; D026902:Potassium Channel Blockers; D013453:Sulfonylurea Compounds; D014665:Vasodilator Agents; C423202:poly(N-p-vinylbenzyl-maltonamide); D008320:Maltose; D000255:Adenosine Triphosphate; C074112:poly-N-4-vinylbenzyllactonamide; D007785:Lactose; D003981:Diazoxide; D005905:Glyburide