Characterization of long cDNA clones from human adult spleen.
As an extension of our analysis of long cDNAs, we here report the characterization of cDNA clones from human adult spleen. From 2000 cDNA clones randomly sampled from a size-fractionated human spleen cDNA library (average size 4.5 kb), 97 clones were selected for sequencing according to their ability to code for protein at the 5'-end sequences and the novelty of their end sequences. The sequence data of these clones demonstrated that 87 out of 97 cDNA clones were derived from independent human genes. The average sizes of the inserts and corresponding open reading frames of these 87 cDNAs reached 4.5 kb and 1.4 kb (corresponding to 468 amino acid residues), respectively. In addition to these sequence analyses in silico, the expression profiles of the genes were also studied in ten human adult tissues by reverse transcription-coupled polymerase chain reaction, products of which were quantified by enzyme-linked immunosorbent assay. The results indicated that spleen could be used as an additional source of human long cDNAs to complement the list of human genes.
Hattori
A
A
Kazusa DNA Research Institute, Kisarazu, Chiba, Japan.
Okumura
K
K
Nagase
T
T
Kikuno
R
R
Hirosawa
M
M
Ohara
O
O
D017398:Alternative Splicing; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D015723:Gene Library; D006801:Humans; D008957:Models, Genetic; D016366:Open Reading Frames; D020161:Physical Chromosome Mapping; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000378:metabolism*; D014018:Tissue Distribution
D018076:DNA, Complementary