Effect of medium-chain fatty acid positional distribution in dietary triacylglycerol on lymphatic lipid transport and chylomicron composition in rats.
The present study was carried out to examine if the positional distribution of medium-chain fatty acid (MCF) in dietary synthetic fat influences lymphatic transport of dietary fat and the chemical composition of chylomicrons in rats with permanent cannulation of thoracic duct. Four types of synthetic triacylglycerol were prepared: (i) sn-1 (3) MCF-sn 2 linoleic acid, (ii) interesterified sn-1(3) MCF-sn 2 linoleic acid, (iii) sn-2 MCF-sn-1(3) linoleic acid, and (iv) interesterified sn-2 MCF-sn-1(3) linoleic acid. A purified diet composed of equal amounts of the synthetic fat and cocoa butter was given to rats with permanent lymph duct cannulation. The positional distribution of MCF in the dietary fat had no significant effect on the lymph flow, triacylglycerol output, phospholipid output, lipid composition of chylomicrons, or the particle size. The positional distribution of MCF in the synthetic triacylglycerol was maintained in the chylomicron triacylglycerol. These results showed that MCF in the dietary triacylglycerol is transported into lymphatics and the positional distribution is well preserved in chylomicron triacylglycerol.
Carvajal
O
O
Division of Bioresource and Bioenvironmental Sciences, Graduate School, Kyushu University, Fukuoka, Japan.
Nakayama
M
M
Kishi
T
T
Sato
M
M
Ikeda
I
I
Sugano
M
M
Imaizumi
K
K
D000818:Animals; D001692:Biological Transport; D001835:Body Weight; D002914:Chylomicrons / Q000378:metabolism*; D004032:Diet; D004041:Dietary Fats / Q000494:pharmacology; D005227:Fatty Acids / Q000737:chemistry* / Q000378:metabolism*; D019787:Linoleic Acid / Q000737:chemistry; D050356:Lipid Metabolism; D008196:Lymph / Q000378:metabolism*; D008198:Lymph Nodes / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014280:Triglycerides / Q000737:chemistry* / Q000378:metabolism
D002914:Chylomicrons; D004041:Dietary Fats; D005227:Fatty Acids; D014280:Triglycerides; C052387:cocoa butter; D019787:Linoleic Acid