Preparative isolation and purification of lutein from the microalga chlorella vulgaris by high-speed counter-current chromatography.
High-speed counter-current chromatography (HSCCC) was applied to the isolation and purification of lutein from microalgae. Analytical HSCCC was used for the preliminary selection of a suitable solvent system composed of n-hexane-ethanol-water (4:3:1, v/v). Using the above solvent system, preparative HSCCC was successfully performed yielding lutein at 98% purity from 200 mg of the crude extract in a one-step separation.
Li
H B
HB
Department of Botany, The University of Hong Kong, China.
Chen
F
F
Zhang
T Y
TY
Yang
F Q
FQ
Xu
G Q
GQ
D002708:Chlorella / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D014975:Lutein / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
D014975:Lutein