Immunotoxicity of cupravit and previcur fungicides in mice.
Female mice received either cupravit or previcur at dose levels of 300 ppm and 1000 ppm, respectively, for 8 weeks. Humoral immune response was evaluated by measuring the plaque-forming cell (PFC) response and hemolysing antibody titers against sheep red blood cells (SRBCs), a T lymphocyte-dependent antigen. In addition, the hemagglutinating antibody titers against Escherichia coli lipopolysaccharide (LPS), a T cell-independent antigen, were determined. Both cupravit and previcur suppressed the PFC response by 74% and 78% of control values, respectively. The primary hemolysin antibody titers were reduced to 62% and 76% of control values, respectively. The hemagglutinating antibody titers were decreased to 66% and 77% of control values, respectively. Memory function was reduced as evidenced by inhibition of secondary hemolysin titers to SRBCs. Cell-mediated immunity was inhibited by both cupravit and previcur as evidenced by reduced delayed-type hypersensitivity (DTH) reaction to tuberculin to 77% and 76% of control values, respectively. Decreases in total leukocytic and lymphocytic counts were recorded together with histopathological changes in spleen and thymus gland of intoxicated animals.
Elsabbagh
H S
HS
Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Cairo University, Egypt.
El-Tawil
O S
OS
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000917:Antibody Formation / Q000187:drug effects; D002219:Carbamates / Q000633:toxicity*; D005260:Female; D005659:Fungicides, Industrial / Q000633:toxicity*; D007109:Immunity / Q000187:drug effects*; D007111:Immunity, Cellular / Q000187:drug effects; D008070:Lipopolysaccharides / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D012756:Sheep; D013154:Spleen / Q000187:drug effects / Q000276:immunology; D013950:Thymus Gland / Q000187:drug effects / Q000276:immunology
D000907:Antibodies, Bacterial; D002219:Carbamates; D005659:Fungicides, Industrial; D008070:Lipopolysaccharides; C033205:propamocarb