Gordonan, an acidic polysaccharide with cell aggregation-inducing activity in insect BM-N4 cells, produced by Gordonia sp.
An acidic polysaccharide, termed gordonan, was isolated from the culture medium of Gordonia sp. as an inducer of cell aggregation in an insect cell line, BM-N4. Gordonan had an average molecular weight of 5 x 10(6) and its structure was identified as -->3)-4-O-(1-carboxyethyl)-beta-D-Manp-(1-->4)-beta-D-GlcAp-(1-->4)-beta-D-Glcp-(1--> mainly by acid hydrolysis experiments and NMR analysis. It induces cell aggregation at the concentration of 4 microg/ml. A partially hydrolyzed polysaccharide derived from gordonan with a molecular weight of 5 x 10(5) showed weak activity, while any fragment molecules with lower molecular weights prepared from gordonan showed no activity.
Kondo
T
T
Department of Applied Biological Chemistry, The University of Tokyo, Japan.
Yamamoto
D
D
Yokota
A
A
Suzuki
A
A
Nagasawa
H
H
Sakuda
S
S
D000192:Actinomycetales / Q000737:chemistry*; D000818:Animals; D002449:Cell Aggregation / Q000187:drug effects; D002478:Cells, Cultured; D005285:Fermentation; D007313:Insecta; D009682:Magnetic Resonance Spectroscopy; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; C421183:gordonan