[Molecular diagnosis and new gene engineering in hematological malignancies].
Advancement molecular diagnostics in hematological malignancy has provided us with a broad menu of new assays and techniques. By integrating the data gleaned from these techniques we can formulate a more rational broad-based diagnosis. Hematological malignancies have traditionally been classified by morphological criteria. However, molecular advances have provided new insights in the genetic backbone of chimeric genes. These malignancies have shown chromosome abnormalities of translocation with chimeric genes, and revealed the rearrangement of chimeric genes by PCR analysis and quantitative PCR system. This review summarized the recent technology for detecting chimeric genes along with concepts of laboratory performance.
Funato
T
T
Department of Molecular Diagnostics, Tohoku University, School of Medicine, Sendai 980-8574.
Fujiwara
J
J
Fujimaki
S
S
Kaku
M
M
D000328:Adult; D002869:Chromosome Aberrations; D025063:Chromosome Disorders; D019411:Clinical Laboratory Techniques; D005260:Female; D015321:Gene Rearrangement; D019337:Hematologic Neoplasms / Q000175:diagnosis* / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007755:Laboratories, Hospital; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000235:genetics
D011993:Recombinant Fusion Proteins