[Usefulness of chromosome alteration analysis in cell lines derived from laryngeal tumors for evaluation of laryngeal neoplasms].
A classical cytogenetic analysis was applied to analyse karyotypes of 9 cell lines derived from laryngeal cancer. The most frequent aberration was a deletion of the whole sex chromosome Y. An abundance of somatic chromosome alterations was further analysed to find correlation with tumour staging and grading. A conventional cytogenetic analysis seems to be not sufficient to recognize chromosome alterations specific for a given tumor stage. On the other hand, an analysis in respect to histologic grading has indicated for an association between rearrangement of 9 chromosome and a high tumor aggressiveness. It seems that a combination of conventional cytogenetics with molecular methods (FISH, CGH) would be helpful in diagnosing of laryngeal cancer.
Jarmuz
M
M
Zakład Genetyki Człowieka PAN w Poznaniu. maljar@yahoo.com
Szyfter
K
K
Grenman
R
R
Golusinski
W
W
Szyfter
W
W
D002460:Cell Line; D002869:Chromosome Aberrations / Q000235:genetics*; D005820:Genetic Testing; D006801:Humans; D007621:Karyotyping; D007822:Laryngeal Neoplasms / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D009367:Neoplasm Staging; D014998:Y Chromosome / Q000235:genetics
Przydatność analizy uszkodzeń chromosomów w liniach komórkowych wyprowadzonych z nowotworów krtani w ocenie przebiegu raka krtani.