Differentiation in murine mastocytoma induced by macrophage gangliosides.
In the membrane of mouse macrophages two gangliosides were detected which inhibit the division of murine mastocytoma P815 tumor cells. The two gangliosides were incorporated into the cytoplasmatic membrane of mastocytoma cells. The concentration necessary to achieve a complete inhibition of P815 tumor cell division is about 1 microM for both effective gangliosides. Macrophage ganglioside-induced inhibition of cell division is accompanied by morphological changes of the mastocytoma cells. While the cells are rounding, their diameter increases and serotonin and granules appear in the cytoplasm of the enlarged cells. Our findings suggest that macrophage gangliosides may differentiate mastocytoma cells into mast cells.
Schaade
L
L
Department of Medical Microbiology, University Hospital, RWTH Aachen, Germany.
Thomssen
R
R
Ritter
K
K
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D048429:Cell Size / Q000187:drug effects / Q000502:physiology; D005732:Gangliosides / Q000494:pharmacology / Q000502:physiology*; D007700:Kinetics; D017737:Macrophages, Peritoneal / Q000502:physiology*; D012515:Mast-Cell Sarcoma / Q000473:pathology*; D051379:Mice; D008811:Mice, Inbred DBA; D008815:Mice, Inbred Strains; D014407:Tumor Cells, Cultured
D005732:Gangliosides