Cloning and characterization of a bean UDP-glucosyltransferase cDNA expressed during plant-bacterial interactions.
A cDNA clone, which corresponds to an RNA transcript that accumulates in bean during the hypersensitive reaction, was isolated and designated Hra25 (for hypersensitive reaction associated). Hra25 is predicted to encode a UDP-glucosyltransferase. Northern analysis was used to study Hra25 transcript accumulation in bean in response to incompatible and compatible strains of Pseudomonas syringae, an Hrp- mutant, and wounding. Our data suggest that the Hra25 transcript is activated in response to specific avr-derived signals as well as non-avr, general signals.
Sullivan
T A
TA
Department of Plant Pathology, North Carolina State University, Raleigh 27695-7616, USA.
Jakobek
J L
JL
Lindgren
P B
PB
D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D007887:Fabaceae / Q000201:enzymology* / Q000235:genetics / Q000382:microbiology*; D015870:Gene Expression; D017343:Genes, Plant; D005964:Glucosyltransferases / Q000235:genetics*; D008969:Molecular Sequence Data; D010946:Plants, Medicinal; D011549:Pseudomonas / Q000472:pathogenicity; D014158:Transcription, Genetic
D018076:DNA, Complementary; D018744:DNA, Plant; D005964:Glucosyltransferases