title: In vitro effect of ethylenediaminetetraacetic acid-Tris on the efficacy of hatchery disinfectants.
abstract: Solutions of ethylenediaminetetraacetic acid (EDTA)-Tris showed synergistic or additive effects on gram-negative bacteria when combined with hatchery disinfectants consisting of phenol and detergent (Magnaphen-100), quaternary ammonium compound (QAC) and glutaraldehyde (Synergize), QAC (BioSentry 904), and hydrogen peroxide. The gram-positive bacteria reacted less favorably, with reaction mixtures showing all three levels of potentiation (synergistie, additive, and antagonistic). Combinations of EDTA-Tris and a commercial glutaraldehyde solution (Glutracide), when mixed with the test organisms, showed mostly antagonistic effects. Solutions of EDTA-Tris decreased the concentration of hatchery disinfectants required for bacterial killing by 75% in those situations in which synergistic potentiation occurred. EDTA-Tris is nontoxic to 12-day-old embryos. Serial passage of the test organisms in solutions of EDTA-Tris did not result in the development of resistant forms.
authors.affiliation: Department of Medical Microbiology and Parasitology, College of Veterinary Medicine, The University of Georgia, Athens 30602, USA.
authors.forename: R E
authors.initials: RE
authors.lastname: Wooley
authors.forename: J E
authors.initials: JE
authors.lastname: Sander
authors.forename: J J
authors.initials: JJ
authors.lastname: Maurer
authors.forename: P S
authors.initials: PS
authors.lastname: Gibbs
mesh_terms: D000822:Animal Husbandry; D000818:Animals; D000831:Animals, Newborn; D002645:Chickens; D004202:Disinfectants / Q000494:pharmacology* / Q000633:toxicity; D004352:Drug Resistance, Microbial / Q000235:genetics; D004357:Drug Synergism; D004492:Edetic Acid / Q000494:pharmacology* / Q000633:toxicity; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D009154:Mutation; D014325:Tromethamine
chemical_list: D004202:Disinfectants; D014325:Tromethamine; D004492:Edetic Acid