Suppression of decidual cell response induced by tributyltin chloride in pseudopregnant rats: a cause of early embryonic loss.
In our previous studies, tributyltin chloride (TBTCl) at doses of 16.3 mg/kg and above caused implantation failure (preimplantation embryonic loss) and postimplantation embryonic loss in rats following administration on gestational day (GD) 0 through GD 3 and GD 4 through GD 7, respectively. This study was designed to assess the effects of TBTCl on uterine function as a cause of early embryonic loss in pseudopregnant rats. TBTCl was given orally to pseudopregnant rats at doses of 4.1, 8.1, 16.3 and 32.5 mg/kg on pseudopregnant day (PPD) 0 to PPD 3 or 8.1, 16.3, 32.5 and 65.1 mg/kg on PPD 4 to PPD 7. The decidual cell response was induced by bilateral scratch trauma on PPD 4. The uterine weight on PPD 9 served as an index of uterine decidualization. Uterine weight and serum progesterone levels on PPD 9 were significantly decreased after administration of TBTCl at doses of 16.3 mg/kg and above on PPD 0 to PPD 3 or PPD 4 to PPD 7. Administration of TBTCl at doses of 8.1 mg/kg and above on PPD 0 to 3 also significantly decreased serum progesterone levels on PPD 4. TBTCl had no effect on ovarian weight and number of corpora lutea. It can be concluded that TBTCl suppresses the uterine decidual cell response and decreases progesterone levels, and these effects are responsible for early embryonic loss due to TBTCl exposure.
Harazono
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National Institute of Health Sciences, Osaka Branch, Japan. harazono@nihs.go.jp
Ema
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D000818:Animals; D003338:Corpus Luteum / Q000187:drug effects / Q000473:pathology; D004435:Eating / Q000187:drug effects; D010064:Embryo Implantation / Q000187:drug effects* / Q000502:physiology; D020964:Embryo Loss / Q000139:chemically induced / Q000209:etiology*; D004958:Estradiol / Q000097:blood; D005260:Female; D005659:Fungicides, Industrial / Q000633:toxicity*; D009929:Organ Size / Q000187:drug effects; D010053:Ovary / Q000187:drug effects / Q000473:pathology; D011374:Progesterone / Q000097:blood; D011555:Pseudopregnancy / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D014220:Trialkyltin Compounds / Q000633:toxicity*; D014599:Uterus / Q000187:drug effects / Q000473:pathology
D005659:Fungicides, Industrial; D014220:Trialkyltin Compounds; D011374:Progesterone; D004958:Estradiol; C011559:tributyltin