title: Large-scale solid-phase preparation of 3'-unprotected trinucleotide phosphotriesters--precursors for synthesis of trinucleotide phosphoramidites.
abstract: The approach to large-scale solid-phase synthesis of 3'-unprotected trinucleotide phosphotriesters has been developed. The trinucleotides have been synthesized in 5 g scale by phosphotriester approach using CPG with pore size 70A. Total yield of target products was 75-90%. The molar extinctions of trinucleotides at various wave-lengths were calculated; the experimental UV-spectra of trinucleotides show a good agreement with theoretical ones. The trinucleotides synthesized were used for synthesis of trinucleotide phosphoramidites - synthons for generation of DNA/peptide libraries.
authors.affiliation: Shemiakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow. kaushin@ibch.siobc.ras.ru
authors.forename: A
authors.initials: A
authors.lastname: Kayushin
authors.forename: M
authors.initials: M
authors.lastname: Korosteleva
authors.forename: A
authors.initials: A
authors.lastname: Miroshnikov
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004952:Esters; D009943:Organophosphorus Compounds / Q000737:chemistry; D013873:Thionucleotides / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D004952:Esters; D009943:Organophosphorus Compounds; D013873:Thionucleotides