Differences in TNF-alpha producing activity from murine peritoneal macrophages induced by lipopolysaccharides of Prevotella heparinolytica and Porphyromonas gingivalis.
We compared the effects of LPSs from P. gingivalis and P. heparinolytica on the induction of tumor necrosis factor-alpha (TNF-alpha) production by murine peritoneal macrophages. The lipopolysaccharide (LPS) preparation from P. gingivalis showed a typical ladder pattern in SDS-PAGE, whereas that from P. heparinolytica formed several stained bands without a ladder pattern. When the macrophages from C3H/HeJ mice were incubated with P. gingivalis LPS, the level of TNF-alpha released in the culture supernatants was significantly higher than that with P. heparinolytica. All tested reagents except genistein strongly inhibited the production of TNF-alpha by the macrophages after induction by either LPS. These results suggested the following possibilities, i) the induction level of TNF-alpha by P. heparinolytica is similar to that of Salmonella minnesota, ii) tyrosine phosphorylation is not the only pathway for the TNF-alpha production induced by these LPSs, iii) different regulatory mechanisms are involved in TNF-alpha production in P. gingivalis LPS stimulated and P. heparinolytica LPS stimulated cells.
Hirai
K
K
Department of Oral Microbiology, Matsumoto Dental University, 1780, Hirooka-Gobara, Shiojiri-shi, Nagano 399-0781, Japan.
Fujimura
S
S
Shibata
Y
Y
Ishihara
K
K
Kato
T
T
Okuda
K
K
Nakamura
T
T
D000818:Animals; D003840:Deoxycholic Acid; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000494:pharmacology; D019833:Genistein / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000032:analysis / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D008815:Mice, Inbred Strains; D010766:Phosphorylation; D016966:Porphyromonas gingivalis / Q000378:metabolism*; D018720:Prevotella / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D012475:Salmonella / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D014443:Tyrosine / Q000378:metabolism
D004791:Enzyme Inhibitors; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D003840:Deoxycholic Acid; D012967:Sodium Dodecyl Sulfate; D014443:Tyrosine; D019833:Genistein; D011505:Protein-Tyrosine Kinases