Bone formation at a rabbit skull defect by autologous bone marrow cells combined with gelatin microspheres containing TGF-beta1.
The objective of the present study is to investigate the addition effect of transforming growth factor (TGF)-beta1 on the bone formation at a rabbit skull defect induced by autologous bone marrow (BM). Following application of gelatin microspheres containing TGF-beta1, with or without BM cells to skull bone defects, bone formation at the defect was assessed by soft X-ray, dual energy X-ray absorptometry (DEXA), and histological examinations. After implantation for 6 weeks, gelatin microspheres containing 0.05 microg of TGF-beta1 plus 10(6) of BM cells induced bone formation at the 6 mm diameter bone defect. The defect was histologically closed by newly formed bone tissue, whilst both gelatin microspheres containing 0.05 microg of TGF-beta1, and 10(6) and 10(7) of BM cells were ineffective. A DEXA experiment revealed that combination of gelatin microspheres containing TGF-beta1 with BM cells enhanced the bone mineral density at the skull defect to a significantly greater extent than other agents. These findings indicate that a combination of gelatin microspheres containing TGF-beta1 enabled BM cells to enhance the osteoinductive ability, resulting in bone formation even at the cell number at which BM cells alone were ineffective.
Tabata
Y
Y
Institute for Frontier Medical Sciences, Kyoto University, Japan. yasuhiko@frontier.kyoto-u.ac.jp
Hong
L
L
Miyamoto
S
S
Miyao
M
M
Hashimoto
N
N
Ikada
Y
Y
D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism*; D001842:Bone and Bones / Q000737:chemistry* / Q000502:physiology*; D002455:Cell Division / Q000187:drug effects; D005780:Gelatin / Q000737:chemistry*; D008863:Microspheres; D011817:Rabbits; D012038:Regeneration / Q000187:drug effects; D012886:Skull / Q000378:metabolism* / Q000473:pathology / Q000502:physiology*; D013977:Tibia / Q000502:physiology; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000008:administration & dosage / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1; D014965:X-Rays
D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D005780:Gelatin