title: The design of a hyperstable mutant of the Abp1p SH3 domain by sequence alignment analysis.
abstract: We have characterized the thermodynamic stability of the SH3 domain from the Saccharomyces cerevisiae Abp1p protein and found it to be relatively low compared to most other SH3 domains, with a Tm of 60 degrees C and a deltaGu of 3.08 kcal/mol. Analysis of a large alignment of SH3 domains led to the identification of atypical residues at eight positions in the wild-type Abp1p SH3 domain sequence that were subsequently replaced by the residue seen most frequently at that position in the alignment. Three of the eight mutants constructed in this way displayed increases in Tm ranging from 8 to 15 degrees C with concomitant increases in deltaGu of up to 1.4 kcal/mol. The effects of these substitutions on folding thermodynamics and kinetics were entirely additive, and a mutant containing all three was dramatically stabilized with a Tm greater than 90 degrees C and a deltaGu more than double that of the wild-type domain. The folding rate of this hyperstable mutant was 10-fold faster than wild-type, while its unfolding rate was fivefold slower. All of the stabilized mutants were still able to bind a target peptide with wild-type affinity. We have analyzed the stabilizing amino acid substitutions isolated in this study and several other similar sequence alignment based studies. In approximately 25% of cases, increased stability can be explained by enhanced propensity of the substituted residue for the local backbone conformation at the mutagenized site.
authors.affiliation: Department of Biochemistry, University of Toronto, Ontario, Canada.
authors.forename: A
authors.initials: A
authors.lastname: Rath
authors.forename: A R
authors.initials: AR
authors.lastname: Davidson
mesh_terms: D000595:Amino Acid Sequence; D016384:Consensus Sequence; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics; D007700:Kinetics; D008840:Microfilament Proteins; D009154:Mutation; D011485:Protein Binding; D011489:Protein Denaturation; D015202:Protein Engineering; D017510:Protein Folding; D017433:Protein Structure, Secondary; D012441:Saccharomyces cerevisiae / Q000737:chemistry; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D013696:Temperature; D013816:Thermodynamics; D018909:src Homology Domains / Q000235:genetics*
chemical_list: C062612:ABP1 protein, S cerevisiae; D005656:Fungal Proteins; D008840:Microfilament Proteins; D029701:Saccharomyces cerevisiae Proteins