[DNA ploidy determination with flow cytometry, Ki-67 index, and overexpression of p53 protein in 121 T1 superficial bladder carcinoma. Retrospective study. Correlation with classic variables].
OBJECTIVE
Observe the correlation between Ki-67 label index, p53 expression and flow cytometry-DNA ploidy with the classic variables (grade, lymphatic permeation, multiplicity, volume, primary).


METHODS
121 superficial bladder tumors T1. 10% Cut-off level for Ki-67 and p53. Aneuploidy is defined as a tumor with DNA index different of 1 or more than 20% in G2-M phase.


RESULTS
Statistical correlation with grade and lymphatic permeation. Ki-67 label index and p53 expression can distinguish between G1, G2 vs G3 and Lx, L0 vs. L1. The volume correlates with positivity to p53.


CONCLUSIONS
Aneuploidy and positivity to Ki-67 and p53 increase with grade and lymphatic permeation.
Moyano Calvo
J L
JL
Servicio de Urología, Hospital Universitario Virgen Macarena, Sevilla.
de Miguel Rodríguez
M
M
Ortiz Gamiz
A
A
Poyato Galán
J M
JM
Sánchez Sánchez
E
E
Blanco Palenciano
E
E
Arribas Rodríguez
J M
JM
Galera Davidson
H
H
Castiñeiras Fernández
J
J
D000368:Aged; D005260:Female; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D019394:Ki-67 Antigen / Q000032:analysis*; D008297:Male; D011003:Ploidies; D012189:Retrospective Studies; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*; D001749:Urinary Bladder Neoplasms / Q000235:genetics*
D019394:Ki-67 Antigen; D016159:Tumor Suppressor Protein p53
Determinación de ploidía de ADN mediante citometría de flujo, índice Ki-67 y sobreexpresión de proteína P53 en 121 carcinomas superficiales de vejiga T1. Estudio retrospectivo. Correlación con las variables clásicas.