[Induction of protective immune responses in rhesus monkey by immunization with recombinant plasmids of polyvalent epitopes of Plasmodium falciparum using cholera toxin B as adjuvant].
The immunogenicity and protective efficacy of the DNA vaccine which include cholera toxin B subunit (CTB) and polyvalent protective epitopes of Plasmodium falciparum (awte gene) was assessed using rhesus monkeys as animal models. Recombinant plasmids of pCMV-CTB-AWTE were given to five rhesus monkeys three times with two weeks intervals by intramuscle (i.m.) route, immunization dose was 500 micrograms per plasmid per animal. High levels of anti-CTB and anti-malaria epitopes antibodies and P. falciparum epitope specific CTL activity were elicited. The vaccinated groups was challenged with 1.25 x 10(8) of P. cynomolgi parasites. All monkeys of the control group was patent for at least 34 days, the DNA vaccinated groups wasn't infected during the 60 days we detected. The cocktail DNA vaccine which contains multi-stage and multi-epitope antigen gene shows excellent immunogenicity and protective efficacy, the results also suggests that DNA vaccine plays an important role against malaria infection.
Zhong
H
H
Molecular Genetics Key Laboratory, Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100850, China.
Cao
C
C
Li
P
P
Zhang
Y H
YH
Shi
Y L
YL
Ma
Q J
QJ
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D002772:Cholera Toxin / Q000276:immunology*; D007114:Immunization; D008253:Macaca mulatta; D017780:Malaria Vaccines / Q000276:immunology*; D016778:Malaria, Falciparum / Q000517:prevention & control; D010957:Plasmids; D010963:Plasmodium falciparum / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology*
D000913:Antibodies, Protozoan; D017780:Malaria Vaccines; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic; D002772:Cholera Toxin