[Effect of hydroxylamine hydrochloride on lipopolysaccharide fatty acid composition in Shigella dysenteriae 1].
Changes in the fatty acid composition of S. dysenteriae 1 lipid A after the treatment of lipopolysaccharide (LPS) with hydrosylamine hydrochloride (HH) and 4 degrees C, 37 degrees C and 56 degrees C were studied with the use of gas-liquid chromatographicmass-spectrometry. The treatment with HH led to a decrease in the toxicity of LPS, but produced no changes in the content of the main fatty acid components of lipid A (lauric, myristic, oxymyristic and palmitic acids). At the same time the total number of minor fatty acid derivatives decreased from 11 (in the original LPS) to 5 in LPS treated with HH at 56 degrees C.
Zakharova
N E
NE
Mechnikov Research Institute for Vaccines and Sera, Research Institute of Immunology, Moscow, Russia.
L'vov
V L
VL
Vaneeva
N P
NP
D000942:Antigens, Bacterial / Q000737:chemistry*; D005227:Fatty Acids / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D019811:Hydroxylamine / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000737:chemistry*; D012762:Shigella dysenteriae / Q000737:chemistry / Q000187:drug effects*
D000942:Antigens, Bacterial; D005227:Fatty Acids; D008070:Lipopolysaccharides; D019811:Hydroxylamine
Vliianie obrabotki solianokislym gidroksilaminom na zhirnokislotnyĭ sostav lipopolisakharida Shigella dysenteriae 1.