Production of free radicals measured by spin trapping during operations for stenosis of the carotid artery.
OBJECTIVE
To analyse production of free radicals during operations for stenosis of the internal carotid artery.


METHODS
University hospital, Sweden.


METHODS
10 patients operated on for carotid artery stenosis.


METHODS
Blood samples were repeatedly drawn from the sigmoid sinus through a catheter in the internal jugular vein on the same side, before, during, and after clamping of the internal carotid artery. OXANOH was added to the blood samples in vitro and the radical production calculated from the amount oxidised to OXANO as electron spin resonance.


METHODS
The relation between radical production and certain clinical variables investigated by partial least squares regression analysis.


RESULTS
There were several significant relations. High systolic blood pressure and advanced age were associated with low, and severe degree of stenosis with increased, free radical production. Certain anaesthetic drugs as well as blood variables also influenced the production of radicals.


CONCLUSIONS
The technique used seems to offer the possibility to find and study methods to reduce free radical production during this kind of operation.
Holm
J
J
Department of Surgery, Sahlgrenska University Hospital, Göteborg, Sweden.
Nilsson
U
U
Waters
N
N
Waters
S
S
Jonsson
O
O
D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000493:Allopurinol / Q000627:therapeutic use; D016893:Carotid Stenosis / Q000601:surgery*; D004791:Enzyme Inhibitors / Q000627:therapeutic use; D005260:Female; D005609:Free Radicals / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008991:Monitoring, Physiologic / Q000379:methods; D015999:Multivariate Analysis; D012044:Regression Analysis; D019097:Spin Trapping / Q000379:methods; D014656:Vascular Surgical Procedures / Q000379:methods; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors
D004791:Enzyme Inhibitors; D005609:Free Radicals; D000493:Allopurinol; D014969:Xanthine Oxidase