Phenotypic distinction and functional characterization of pro-B cells in adult mouse bone marrow.
A lymphoid-committed progenitor population was isolated from mouse bone marrow based on the cell surface phenotype Thy-1.1(neg)Sca-1(pos)c-Kit(low)Lin(neg). These cells were CD43(pos)CD24(pos) on isolation and proliferated in response to the cytokine combination of steel factor, IL-7, and Flt3 ligand. Lymphoid-committed progenitors could be segregated into more primitive and more differentiated subsets based on expression of AA4.1. The more differentiated subset generated only B lymphoid cells in 92% of total colonies assayed, lacked T lineage potential, and expressed Pax5. These studies have therefore defined and isolated a B lymphoid-committed progenitor population at a developmental stage corresponding to the initial expression of CD45R.
Mojica
M P
MP
Department of Human Genetics, University of Utah, Salt Lake City, UT 84132, USA.
Perry
S S
SS
Searles
A E
AE
Elenitoba-Johnson
K S
KS
Pierce
L J
LJ
Wiesmann
A
A
Slayton
W B
WB
Spangrude
G J
GJ
D000375:Aging / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte / Q000096:biosynthesis; D016175:B-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D019070:Cell Lineage / Q000235:genetics / Q000276:immunology; D002469:Cell Separation; D002478:Cells, Cultured; D018507:Gene Expression Regulation, Developmental / Q000276:immunology; D018960:Hyaluronan Receptors; D016130:Immunophenotyping; D008562:Membrane Glycoproteins; D051379:Mice; D008806:Mice, Inbred AKR; D008810:Mice, Inbred C57BL; D024101:Mitochondrial Proteins; D022423:Myeloid Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D019009:Proto-Oncogene Proteins c-kit / Q000096:biosynthesis; D011951:Receptors, Complement / Q000096:biosynthesis; D013234:Stem Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D018800:Thy-1 Antigens / Q000096:biosynthesis
D000944:Antigens, Differentiation, B-Lymphocyte; C490193:C1qbp protein, mouse; D018960:Hyaluronan Receptors; D008562:Membrane Glycoproteins; D024101:Mitochondrial Proteins; D011951:Receptors, Complement; D018800:Thy-1 Antigens; C032402:complement 1q receptor; D019009:Proto-Oncogene Proteins c-kit