Morphogenesis in short-term and long-term anther derived calli of Oenothera hookeri de vries.
Anther culture of O. hookeri on Murashige and Skoog (1962) medium supplemented with 2 mg l-1 2,4-dichlorophenoxyacetic acid and 2 mg-1 1-naphthaleneacetic acid produced callus formation. When subcultured onto medium lacking auxin, the callus regenerated through the organogenic pathway. Non-organogenic and organogenic callus was observed using histological methods after 2, 3 and 24 weeks in culture. Three types of calli were recognized: non-organogenic friable calli, organogenic friable calli with roots and organogenic hard calli with shoots. The microscopical sections showed striking differences in tissue organization among friable and compact calli. Vascular bundles were prominent in compact calli, but were not found in friable calli. Calli sections showed at light microscopy cells at two developmental stages; differentiated highly vacuolated cells and meristematic small isodiametric cells with densely stained cytoplasm. At electron microscopy level abnormal chloroplasts were present in non-organogenic calli, while chloroplasts were well developed in organogenic hard calli. Peroxisomes with paracrystalline protein bodies were abundant in both types of calli.
Martínez
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LD
Centro de Microscopía Electrónica, Universidad Católica de Córdoba, Obispo Trejo 323, (5000) Córdoba, Argentina. dormar50@hotmail.com
Noher de Halac
I
I
D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D002736:Chloroplasts / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D009280:Naphthaleneacetic Acids / Q000494:pharmacology; D018521:Plant Physiological Phenomena / Q000187:drug effects*; D010946:Plants, Medicinal / Q000187:drug effects* / Q000254:growth & development* / Q000648:ultrastructure; D012038:Regeneration / Q000187:drug effects / Q000502:physiology; D012639:Seeds / Q000187:drug effects* / Q000254:growth & development* / Q000648:ultrastructure
D009280:Naphthaleneacetic Acids; D015084:2,4-Dichlorophenoxyacetic Acid; C034182:1-naphthaleneacetic acid