title: Vibrio cyclotrophicus sp. nov., a polycyclic aromatic hydrocarbon (PAH)-degrading marine bacterium.
abstract: Strain P-2P44T was isolated from creosote-contaminated marine sediments by using a most-probable number procedure in which phenanthrene was the sole carbon and energy source. Growth experiments showed that P-2P44T utilized several two- and three-ring polycyclic aromatic hydrocarbons (PAHs) as substrates, including naphthalene, 2-methylnaphthalene and phenanthrene. Additionally, gas-chromatography experiments showed that P-2P44T degraded several other PAHs, though it was unable to use them as sole sources of carbon and energy. Phylogenetic analyses confirmed that strain P-2P44T is a member of the genus Vibrio, most closely related to Vibrio splendidus. However, strain P-2P44T shared only 98.3% 16S rDNA identity and 35% DNA-DNA reassociation with the type strain of V. splendidus. Strain P-2P44T differed phenotypically from V. splendidus. Together, these differences indicated that strain P-2P44T represents a novel species in the genus Vibrio, for which the name Vibrio cyclotrophicus sp. nov. is proposed; the type strain is P-2P44T (= ATCC 700982T = PICC 106644T).
authors.forename: B P
authors.initials: BP
authors.lastname: Hedlund
authors.forename: J T
authors.initials: JT
authors.lastname: Staley
mesh_terms: D015373:Bacterial Typing Techniques; D001673:Biodegradation, Environmental; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D019015:Geologic Sediments / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology; D014733:Vibrio / Q000145:classification* / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D014877:Water Pollution, Chemical
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D011084:Polycyclic Aromatic Hydrocarbons; D012336:RNA, Ribosomal, 16S