A DNA marker for the duplicated cytosolic PGI genes in sheep's fescue (Festuca ovina L.).
Active duplicate PgiC genes in sheep's fescue, Festuca ovina, are associated with a PCR marker of specific length (about 370 bp, of which 231 are in an intron). Using this marker, the frequency of plants with duplicate genes is estimated to be about 10% in a population from southern Sweden. The close molecular similarity between the electrophoretically different duplicated genes is in accordance with the conclusion reached earlier that they are indeed alleles at the same locus.
Ghatnekar
L
L
Department of Genetics, Lund University, Sölvegatan 29, SE-223 62 Lund, Sweden.
Bengtsson
B O
BO
D000483:Alleles; D003600:Cytosol; D017931:DNA Primers / Q000737:chemistry; D018744:DNA, Plant / Q000032:analysis; D004171:Diploidy; D020440:Gene Duplication; D017343:Genes, Plant / Q000235:genetics*; D005819:Genetic Markers; D014644:Genetic Variation; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics*; D007438:Introns; D006109:Poaceae / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers; D005956:Glucose-6-Phosphate Isomerase