title: Purification method for the isolation of monophosphate nucleotides from Champagne wine and their identification by mass spectrometry.
abstract: Monophosphate nucleotides are difficult to identify in Champagne wine because they are present in small concentrations in a complex mixture. A method for the isolation, separation and identification of reference compounds, which achieved on average 79% recovery (except for cytidine derivatives), was developed and applied to wine. Some monophosphate nucleotides were then isolated from a Champagne wine aged on lees for 8 years, by ultrafiltration followed by a semi-preparative HPLC step using a strong anion-exchange column. The fraction obtained was subjected to HPLC in a reversed-phase column to remove the salt previously introduced, before identification of compounds by HPLC coupled to a mass spectrometer. For the first time in wine, 5'-IMP, 5'-AMP, 5'-CMP, 5'-GMP, 5'-UMP and the 3'- and/or 2'-isomers of the four latter compounds were identified by comparing their HPLC and electrospray ionization mass spectrometry data with those of reference nucleotides.
authors.affiliation: Université de Bourgogne, Institut Universitaire de la Vigne et du Vin, Dijon, France.
authors.forename: J
authors.initials: J
authors.lastname: Aussenac
authors.forename: D
authors.initials: D
authors.lastname: Chassagn
authors.forename: C
authors.initials: C
authors.lastname: Claparols
authors.forename: M
authors.initials: M
authors.lastname: Charpentier
authors.forename: B
authors.initials: B
authors.lastname: Duteurtre
authors.forename: M
authors.initials: M
authors.lastname: Feuillat
authors.forename: C
authors.initials: C
authors.lastname: Charpentier
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D009711:Nucleotides / Q000737:chemistry / Q000302:isolation & purification*; D014920:Wine / Q000032:analysis*
chemical_list: D009711:Nucleotides