Neutrophil migration and aggregation induced by euphorbin, a lectin from the latex of Euphorbia milii, var. milii.
OBJECTIVE
To study the neutrophil migration and aggregation induced by euphorbin, a D-galactose binding lectin from Euphorbia milii var. milli latex.


METHODS
Euphorbin-induced neutrophil migration was evaluated in vivo and in vitro, in the absence or presence of soluble D-galactose. Neutrophil aggregation induced in vitro by euphorbin was determined by light microscopy.


RESULTS
The neutrophil migration inducing activity of euphorbin was dose-dependent and inhibited by soluble D-galactose. Neutrophil aggregation was rapidly reversed when provoked by 0.1 mg/ml euphorbin. In higher concentrations, euphorbin caused persistent and more extensive neutrophil aggregation.


CONCLUSIONS
Euphorbin induced neutrophil migration through its sugar recognition property. The transitory neutrophil aggregation, induced by a euphorbin quantity similar to that able to cause maximal chemotactic response, is characteristic of homotypic neutrophil adhesion, whereas persistent aggregation, provoked by higher euphorbin quantities, corresponds to cell agglutination by a multivalent lectin.
Dias-Baruffi
M
M
Department of Clinical Analysis, Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of Sao Paulo, Brazil.
Sakamoto
M
M
Rossetto
S
S
Vozári-Hampe
M M
MM
Roque-Barreira
M C
MC
D000818:Animals; D002449:Cell Aggregation / Q000187:drug effects; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D019665:Euphorbiaceae / Q000737:chemistry*; D005690:Galactose / Q000494:pharmacology; D007840:Latex / Q000737:chemistry; D037102:Lectins / Q000494:pharmacology*; D008297:Male; D009504:Neutrophils / Q000187:drug effects*; D010529:Peritoneal Cavity / Q000166:cytology; D037121:Plant Lectins; D051381:Rats; D017208:Rats, Wistar
D007840:Latex; D037102:Lectins; D037121:Plant Lectins; C425284:euphorbin; D005690:Galactose