[Analysis of the functional role in behavior of the neuromediator receptor by antisense knockdown of its gene expression].
Now available nucleotide sequences of neurotransmitter receptor genes enable to apply oligonucleotides targeted to mRNAs of these genes for highly selective inactivation of their expression (antisense-knockdown) and for function determination of single receptor subtype by this experimental approach. The antisense-knockdown may be of special importance in case of receptor families members of which are pharmacologically similar. Advantages of the antisense technology for investigation into the brain neurotransmitter receptor function in regulation of behaviour, are discussed.
Dygalo
N N
NN
Institute of Cytology and Genetics of the Russian Acad. Sci., Siberian Branch, Russia, 630090, Novosibirsk.
Kalinina
T S
TS
Shishkina
G T
GT
D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D001921:Brain / Q000378:metabolism*; D015870:Gene Expression; D016372:RNA, Antisense / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics*; D017981:Receptors, Neurotransmitter / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology
D016372:RNA, Antisense; D012333:RNA, Messenger; D017981:Receptors, Neurotransmitter
Analiz povedencheskikh éffektov retseptora neĭromediatora putem antisens-nokdauna ékspressii ego gena.