ELISA-based method for determining the affinity of bivalent antibodies of two specificities in a mixture.
The problem of the evaluation of the affinity for two types of bivalent antibodies in a mixture is considered. It is shown that the binding curve in appropriate coordinates can be used to compose either a system of four equations with four unknowns or a system of two equations with two unknown variables. The numerical solution of these equation systems yields affinity constants for both antibodies and the relationship between concentrations of antibodies studied in the mixture.
Bobrovnik
S A
SA
Gulbenkian Institute of Science, Rua da Quinta Granda, 6, Apartado 14, 2780-901 Oeiras, Portugal. sergiy@igc.gulbenKian.pt
D018033:Antibodies, Bispecific / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000915:Antibody Affinity; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*
D018033:Antibodies, Bispecific; D000911:Antibodies, Monoclonal