DNA of nonhuman primates harbors hepatitis C-virus-specific sequences of its 5'-non-coding region (5'-NCR).
The DNA from PBMCs of both hepatitis C virus (HCV)-positive patients and healthy HCV-negative human individuals tested thus far contains essential parts--up to 272/341 nucleotides--of the HCV 5'-non-coding region (5'-NCR). These findings bring up the question of the possible evolutionary background of these sequences. Therefore, using the same methodology, we looked for the same sequences in animals closely related to man, i.e., in nonhuman primates (two chimpanzees, one orang-utan, one Debrazza monkey, two New World monkey species and a prosimian). The DNA from PBMCs of the studied animals belonging to nonhuman primates contains essential parts--up to 272/341 nucleotides--of the HCV 5'-non-coding region (5'-NCR). A common sequence of 82 nucleotides is contained in the DNA of all the tested animals but only the chimpanzee's DNA harbors the same, longer sequence region of 272/341 nucleotides of the 5'-NCR found in human DNA. The results may provide a clue as to the possible origin of parts of the IRES containing sequence area of the HCV.
Dennin
R H
RH
Institute of Medical Microbiology and Hygiene, Medical University, Luebeck, Germany.   dennin@hygiene.mu-luebeck.de
Wo
J
J
Chen
Z
Z
Roos
C
C
D000818:Animals; D002427:Cebidae; D002520:Cercopithecidae; D004279:DNA, Viral / Q000235:genetics*; D019143:Evolution, Molecular; D016174:Hepacivirus / Q000235:genetics*; D006526:Hepatitis C / Q000821:virology*; D006801:Humans; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D022661:RNA, Untranslated / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D004279:DNA, Viral; D022661:RNA, Untranslated