Swallowing reflex and brain stem neurons activated by superior laryngeal nerve stimulation in the mouse.
The purpose of the present study was to identify vagal subnuclei that participate in reflex swallowing in response to electrical stimulation of the left superior laryngeal nerve (SLN). SLN stimulation at 10 Hz evoked primary peristalsis, including oropharyngeal and esophageal peristalsis, and LES relaxation. It also induced c-fos expression in interneurons in the interstitial (SolI), intermediate (SolIM), central (SolCe), dorsomedial (SolDM) and commissural (SolC) solitary subnuclei. Neurons in parvicellular reticular nucleus (PCRt) and area postrema (AP) and motoneurons in the semicompact (NAsc), loose (NAl), and compact (NAc) formations of the nucleus ambiguus and both rostral (DMVr) and caudal (DMVc) parts of the dorsal motor nucleus of vagus were also activated. The activated neurons represent all neurons concerned with afferent SLN-mediated reflexes, including the swallowing-related neurons. SLN stimulation at 5 Hz elicited oropharyngeal and LES but not esophageal responses and evoked c-fos expression in neurons in SolI, SolIM, SolDM, PCRt, AP, NAsc, NAl, and DMVc but not in SolCe, NAc, or DMVr. These data are consistent with the role of SolI, SolIM, SolDM, NAsc, NAl, and DMVc circuit in oropharyngeal peristalsis and LES relaxation and SolCe, NAc, DMVc, and DMVr in esophageal peristalsis and LES responses.
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Center for Swallowing and Motility Disorders, West Roxbury Division of Veterans Affairs Boston Healthcare System, West Roxbury, Massachusetts 02132, USA.
Goyal
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D000818:Animals; D001933:Brain Stem / Q000166:cytology / Q000502:physiology*; D003679:Deglutition / Q000502:physiology*; D004558:Electric Stimulation; D004943:Esophagogastric Junction / Q000502:physiology; D004947:Esophagus / Q000502:physiology; D007823:Laryngeal Nerves / Q000502:physiology*; D008297:Male; D051379:Mice; D009474:Neurons / Q000502:physiology*; D010528:Peristalsis / Q000502:physiology; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D012018:Reflex / Q000502:physiology*; D017552:Solitary Nucleus / Q000502:physiology
D016760:Proto-Oncogene Proteins c-fos