Molecular typing of Trichomonas vaginalis isolates by HSP70 restriction fragment length polymorphism.
Subtyping isolates of Trichomonas vaginalis is an essential tool for understanding the epidemiology of this common sexually-transmitted disease. Restriction fragment length polymorphism (RFLP) analysis employing a probe from the heat-inducible cytoplasmic HSP70 gene family hybridized with EcoR I-digested genomic DNA was used in the molecular typing of Trichomonas isolates. Analysis of five American Type Culture Collection (ATCC) reference strains and 31 Jackson, Mississippi, isolates from six male and 21 female patients, revealed 10 distinct RFLP pattern subtypes of Trichomonas. The subtypes were temporally stable and cosmopolitan. The RFLP profiles seen in Maryland, Ohio, Massachusetts, and New York ATCC strains were identical to those of some Mississippi isolates, even though the samples were isolated 10-35 years apart. There was no correlation between metronidazole resistance and RFLP subtype with resistant isolates from eight patients distributed among six different subtypes.
Stiles
J K
JK
Department of Medicine, University of Mississippi Medical Center, Jackson 39216-4505, USA.
Shah
P H
PH
Xue
L
L
Meade
J C
JC
Lushbaugh
W B
WB
Cleary
J D
JD
Finley
R W
RW
D000818:Animals; D000994:Antitrichomonal Agents / Q000494:pharmacology; D015139:Blotting, Southern; D004262:DNA Restriction Enzymes / Q000378:metabolism; D016054:DNA, Protozoan / Q000737:chemistry; D004351:Drug Resistance; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D008297:Male; D008795:Metronidazole / Q000494:pharmacology; D012150:Polymorphism, Restriction Fragment Length; D014245:Trichomonas Infections / Q000469:parasitology*; D014247:Trichomonas Vaginitis / Q000469:parasitology; D014246:Trichomonas vaginalis / Q000145:classification* / Q000187:drug effects / Q000235:genetics; D014526:Urethritis / Q000469:parasitology; D014555:Urination Disorders / Q000469:parasitology
D000994:Antitrichomonal Agents; D016054:DNA, Protozoan; D018840:HSP70 Heat-Shock Proteins; D008795:Metronidazole; D004262:DNA Restriction Enzymes