SPLASH (PLA2IID), a novel member of phospholipase A2 family, is associated with lymphotoxin deficiency.
Lymphotoxin (LT) deficient mice have profound defects in the splenic microarchitecture associated with defective expression on certain gene products, including chemokines. By using subtraction cloning of splenic cDNA from wild-type and LT alpha or TNF/LT alpha double deficient mice we isolated a novel murine gene encoding a secretory type phospholipase A2, called SPLASH. The two major alternative transcripts of SPLASH gene are predominantly expressed in lymphoid tissues, such as spleen and lymph nodes. SPLASH maps to the distal part of chromosome 4, to which several cancer-related loci have been also mapped.
Shakhov
A N
AN
Intramural Research Support Program, SAIC Frederick and Laboratory of Molecular Immunoregulation, Division of Basic Sciences, NCI-FCRDC, Frederick, MD 21702, USA. shakhova@mail.ncifcrf.gov
Rubtsov
A V
AV
Lyakhov
I G
IG
Tumanov
A V
AV
Nedospasov
S A
SA
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D054501:Group II Phospholipases A2; D006801:Humans; D008221:Lymphoid Tissue / Q000201:enzymology / Q000276:immunology; D008233:Lymphotoxin-alpha / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D010741:Phospholipases A / Q000235:genetics*; D054467:Phospholipases A2; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D017931:DNA Primers; D018076:DNA, Complementary; D008233:Lymphotoxin-alpha; D010741:Phospholipases A; D054501:Group II Phospholipases A2; C516293:PLA2G2D protein, human; D054467:Phospholipases A2; C516294:Pla2g2d protein, mouse