Detection and differentiation of CAV-1 and CAV-2 by polymerase chain reaction.
Canine adenovirus type 1 (CAV-1) and type 2 (CAV-2) can be categorized in the laboratory by haemagglutination and neutralization tests, but they are difficult to differentiate from each other in specimens, especially when infection occurs in the digestive tract. The object of this study was to develop a simple method of detecting and differentiating them. One pair of common primers was designed and synthesized according to the sequences of the E3 and flanking regions and a polymerase chain reaction (PCR) assay was established using these two primers to amplify the virus-specific DNA fragment from clinical specimens as well as from cell cultures. After elecctrophoresis, under the same amplification conditions, 508 bp and 1030 bp PCR products were observed for CAV-1 and CAV-2, respectively. These were further shown to be adenovirus specific by dot hybridization and sequencing. As only one pair of primers was involved in the PCR procedure, it was faster and easier to perform than any of the other assays used for detecting canine adenovirus, making it applicable in the rapid confirmation of diagnosis and differentiation of the two types of canine adenoviruses.
Hu
R L
RL
Laboratory of Virology, Veterinary Institute, Changchun University of Agriculture and Animal Sciences, China.
Huang
G
G
Qiu
W
W
Zhong
Z H
ZH
Xia
X Z
XZ
Yin
Z
Z
D000257:Adenoviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D006516:Adenoviruses, Canine / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D003588:Cytopathogenic Effect, Viral; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000737:chemistry / Q000302:isolation & purification; D003937:Diagnosis, Differential; D004283:Dog Diseases / Q000145:classification / Q000175:diagnosis / Q000821:virology*; D004285:Dogs; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D006522:Hepatitis, Infectious Canine / Q000175:diagnosis / Q000821:virology; D007827:Laryngitis / Q000175:diagnosis / Q000662:veterinary / Q000821:virology; D008854:Microscopy, Electron / Q000662:veterinary; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D017422:Sequence Analysis, DNA; D014136:Tracheitis / Q000175:diagnosis / Q000662:veterinary / Q000821:virology
D017931:DNA Primers; D004279:DNA, Viral