title: Immunohistochemical observation of actin filaments in epithelial cells encircling the taste pore cavity of rat fungiform papillae.
abstract: Epithelial cells are connected to each other around taste pores in rat fungiform papillae. Cytoskeletal components are responsible for the maintenance of intracellular adhesion, and we investigated the identification and localization of actin filaments around taste pores. On the basis of observations made by immunohistochemical transmission electron microscopy comparing with confocal laser scanning microscopy using actin-lectin double staining, actin filaments were found to be localized, encircling the squeezed taste pore cavity, in epithelial cells a few micrometers below the papilla surface. In addition, these observations suggest that the organization of actin filaments around taste pores might be involved in the constriction of taste pores.
authors.affiliation: Department of Oral Physiology, Hiroshima University School of Dentistry, Japan. fvgn7971@mb.infoweb.ne.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Ohishi
authors.forename: S
authors.initials: S
authors.lastname: Komiyama
authors.forename: K
authors.initials: K
authors.lastname: Wakida
authors.forename: T
authors.initials: T
authors.lastname: Uchida
authors.forename: Y
authors.initials: Y
authors.lastname: Shiba
mesh_terms: D008841:Actin Cytoskeleton / Q000648:ultrastructure*; D000199:Actins / Q000378:metabolism; D000818:Animals; D004847:Epithelial Cells / Q000648:ultrastructure; D016650:Fluorescein-5-isothiocyanate; D019084:Fluorescent Antibody Technique, Indirect; D005456:Fluorescent Dyes; D037102:Lectins / Q000378:metabolism; D008297:Male; D008855:Microscopy, Electron, Scanning / Q000379:methods; D008867:Microtomy; D010590:Phalloidine; D037121:Plant Lectins; D051381:Rats; D017208:Rats, Wistar; D012235:Rhodamines; D013194:Staining and Labeling / Q000379:methods; D013650:Taste Buds / Q000648:ultrastructure*
chemical_list: D000199:Actins; D005456:Fluorescent Dyes; D037102:Lectins; D037121:Plant Lectins; D012235:Rhodamines; C036850:Ulex europaeus lectins; D010590:Phalloidine; D016650:Fluorescein-5-isothiocyanate