title: In vitro propagation of Acorus calamus Linn.--a medicinal plant.
abstract: High frequency in vitro propagation protocol was standardized from rhizome explants of A. calamus. Maximum shoot multiplication frequency was obtained on Murashige and Skoog's media supplemented with 4 mg/l 6-benzyl amino purine and 0.5 mg/l indole-3-acetic acid. Regenerated shoots were rooted in vitro or directly transferred to sterile soil and well developed roots were observed within two weeks. The rooted plants were successfully established in the field.
authors.affiliation: Centre for Plant Molecular Biology, Osmania University, Hyderabad 500 007, India.
authors.forename: A S
authors.initials: AS
authors.lastname: Rani
authors.forename: V V
authors.initials: VV
authors.lastname: Subhadra
authors.forename: V D
authors.initials: VD
authors.lastname: Reddy
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D001593:Benzyl Compounds; D001901:Botany / Q000379:methods; D007210:Indoleacetic Acids / Q000494:pharmacology; D007701:Kinetin; D019684:Magnoliopsida / Q000187:drug effects / Q000254:growth & development*; D018517:Plant Roots / Q000254:growth & development; D018520:Plant Shoots / Q000254:growth & development; D010946:Plants, Medicinal / Q000187:drug effects / Q000254:growth & development*; D011687:Purines
chemical_list: D001593:Benzyl Compounds; D007210:Indoleacetic Acids; D011687:Purines; C030737:indoleacetic acid; D000225:Adenine; C480551:benzylaminopurine; D007701:Kinetin