A single neuron response to photodynamic effect of various aluminum and zinc phthalocyanines.
The photodynamic effects of sulphonated zinc and aluminum phthalocyanine derivatives as well as phosphonated aluminum phthalOcyanine on the firing of isolated crayfish mechanoreceptor neurons were studied. After 30 min staining neurons were irradiated with He-Ne laser (632.8 nm, 0.3 W/cm2) and changes in neuron firing frequency were recorded. Neuron firing was found to be very sensitive to photodynamic effect and could serve as a sensitive indicator of cell photodamage. It changed the firing level and then died at nanomolar concentrations of phthalocyanines. The dynamics of the neuron responses to photodynamic effects included stages of firing activation and/or inhibition prior to irreversible firing abolition. The order of these stages depended on photosensitizer type and concentration. The comparison of the dependencies of neuron lifetime on photosensitizer concentrations showed ZnPcS2 to be the most effective photosensitizer.
Uzdensky
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AB
Rostov University, Department of Biophysics, Rostov-on-Don, Russia. uzd@krinc.ru
Derkacheva
V M
VM
Dergacheva
O Y
OY
Zhavoronkova
A A
AA
D000535:Aluminum / Q000494:pharmacology; D000818:Animals; D003400:Astacoidea; D066298:In Vitro Techniques; D007211:Indoles / Q000494:pharmacology*; D054833:Isoindoles; D007834:Lasers; D008465:Mechanoreceptors / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D009942:Organometallic Compounds / Q000494:pharmacology*; D017319:Photosensitizing Agents / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D015032:Zinc / Q000494:pharmacology; D017967:Zinc Compounds
D007211:Indoles; D054833:Isoindoles; D009942:Organometallic Compounds; D017319:Photosensitizing Agents; D017967:Zinc Compounds; C052159:Zn(II)-phthalocyanine; C060570:aluminum phthalocyanine; D000535:Aluminum; D015032:Zinc