Expression of neural cell adhesion molecule in spinal cords following a complete transection.
Neural cell adhesion molecule (NCAM) regulates tissue organization during development and in the adult. NCAM upregulation occurs after an injury to brains and sciatic nerves. However, little is known about NCAM expression after spinal cord injury (SCI). By using a complete spinal cord transection with a 5 mm tissue removal, an increase in the NCAM level is detected in spinal cord stumps proximal and distal to the transection site at 1 d and 3 d post injury, while its expression at 8 d is declined to a lower level than that observed in sham-operated spinal cords. The strong NCAM expression is present in motor neurons at 3 d post transection whereas the intensive NCAM immunostaining is localized in dorsal sensory and corticospinal fiber tracts at 8 d following injury. Collectively, NCAM level is elevated and strongly expressed in dorsal fiber tracts after SCI, implying that the endogenous process for spinal cord regeneration may take place after SCI.
Tzeng
S F
SF
Department of Research and Education, Taichung Veterans General Hospital, Taiwan. stzeng@vghtc.vghtc.gov.tw
Cheng
H
H
Lee
Y S
YS
Wu
J P
JP
Hoffer
B J
BJ
Kuo
J S
JS
D000818:Animals; D015153:Blotting, Western; D004195:Disease Models, Animal; D005260:Female; D007150:Immunohistochemistry; D009416:Nerve Regeneration; D019006:Neural Cell Adhesion Molecules / Q000096:biosynthesis*; D020033:Protein Isoforms / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000378:metabolism* / Q000502:physiology; D013119:Spinal Cord Injuries / Q000378:metabolism*
D019006:Neural Cell Adhesion Molecules; D020033:Protein Isoforms