Different mechanisms of radiation-induced loss of heterozygosity in two human lymphoid cell lines from a single donor.
Allelic loss is an important mutational mechanism in human carcinogenesis. Loss of heterozygosity (LOH) at an autosomal locus is one outcome of the repair of DNA double-strand breaks (DSBs) and can occur by deletion or by mitotic recombination. We report that mitotic recombination between homologous chromosomes occurred in human lymphoid cells exposed to densely ionizing radiation. We used cells derived from the same donor that express either normal TP53 (TK6 cells) or homozygous mutant TP53 (WTK1 cells) to assess the influence of TP53 on radiation-induced mutagenesis. Expression of mutant TP53 (Met 237 Ile) was associated with a small increase in mutation frequencies at the hemizygous HPRT (hypoxanthine phosphoribosyl transferase) locus, but the mutation spectra were unaffected at this locus. In contrast, WTK1 cells (mutant TP53) were 30-fold more susceptible than TK6 cells (wild-type TP53) to radiation-induced mutagenesis at the TK1 (thymidine kinase) locus. Gene dosage analysis combined with microsatellite marker analysis showed that the increase in TK1 mutagenesis in WTK1 cells could be attributed, in part, to mitotic recombination. The microsatellite marker analysis over a 64-cM region on chromosome 17q indicated that the recombinational events could initiate at different positions between the TK1 locus and the centromere. Virtually all of the recombinational LOH events extended beyond the TK1 locus to the most telomeric marker. In general, longer LOH tracts were observed in mutants from WTK1 cells than in mutants from TK6 cells. Taken together, the results demonstrate that the incidence of radi-ation-induced mutations is dependent on the genetic background of the cell at risk, on the locus examined, and on the mechanisms for mutation available at the locus of interest.
Wiese
C
C
Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720 USA.
Gauny
S S
SS
Liu
W C
WC
Cherbonnel-Lasserre
C L
CL
Kronenberg
A
A
D000483:Alleles; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004252:DNA Mutational Analysis; D020450:Heavy Ions; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D007501:Iron; D019656:Loss of Heterozygosity / Q000528:radiation effects*; D008214:Lymphocytes / Q000166:cytology / Q000502:physiology / Q000528:radiation effects*; D008938:Mitosis / Q000235:genetics / Q000528:radiation effects; D016296:Mutagenesis / Q000528:radiation effects; D011995:Recombination, Genetic / Q000528:radiation effects*; D013937:Thymidine Kinase / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000502:physiology
D016159:Tumor Suppressor Protein p53; D004247:DNA; D007501:Iron; D007041:Hypoxanthine Phosphoribosyltransferase; D013937:Thymidine Kinase; C092717:thymidine kinase 1