Prooxidant role of histidine in hypoxic stressed mice and Fe(3+)-induced lipid peroxidation.
An attempt was made to study the effect of histidine on reactive oxygen species in a rodent model of hypoxic stress and in Fe(3+)-ascorbic acid-induced lipid peroxidation in mouse brain homogenates. The latency for onset of hypoxic stress-induced convulsions was decreased in histidine-treated animals with a concomitant rise in brain lipid peroxidation levels. In vitro, histidine potentiated Fe(3+)-ascorbic acid-induced lipid peroxidation in mouse brain homogenates while other antioxidants like B-HT and U-74500A inhibited the same. Moreover, Fe(3+)-histidine-induced lipid peroxidation could not be inhibited by preincubation of the system with high concentrations of ascorbic acid. Thus, it is concluded that histidine acts as a strong prooxidant potentiating the genesis of reactive oxygen species during hypoxic stress as well as during Fe(3+)-ascorbic acid-induced lipid peroxidation.
Paul
V N
VN
Pharmacology Division, University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh, India.
Chopra
K
K
Kulkarni
S K
SK
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D002712:Chlorides; D005290:Ferric Compounds / Q000494:pharmacology*; D006639:Histidine / Q000494:pharmacology*; D002534:Hypoxia, Brain / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects* / Q000502:physiology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D017382:Reactive Oxygen Species / Q000502:physiology; D013312:Stress, Physiological / Q000378:metabolism / Q000503:physiopathology
D000975:Antioxidants; D002712:Chlorides; D005290:Ferric Compounds; D017382:Reactive Oxygen Species; D006639:Histidine; D008315:Malondialdehyde; D001205:Ascorbic Acid; C024555:ferric chloride