title: Effect of liquid chromatography separation of complex matrices on liquid chromatography-tandem mass spectrometry signal suppression.
abstract: The effect of liquid chromatography separation on liquid chromatography-tandem mass spectrometry (LC-MS-MS) signal response for the characterization of low-molecular-mass compounds in a complex matrix was investigated. Matrix induced signal suppression appears throughout the entire LC-MS-MS analysis of wheat forage extract, with greatest suppression occurring at early retention times. Experimental results show that co-elution of matrix components and analytes from the LC column may be most strongly attributed to column overloading rather than similar analyte and matrix retention behavior. As a result, two-dimensional (LC-LC) separation can be a highly effective approach to address signal suppression effects for the quantitative LC-MS-MS analysis of complex matrix samples.
authors.affiliation: Department of Chemistry, Vanderbilt University, Nashville, TN 37235, USA.
authors.forename: B K
authors.initials: BK
authors.lastname: Choi
authors.forename: D M
authors.initials: DM
authors.lastname: Hercules
authors.forename: A I
authors.initials: AI
authors.lastname: Gusev
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D014908:Triticum / Q000737:chemistry*