Immunolocalization of interleukin-8 and proliferating cell nuclear antigen in gingival keratinocytes in patients with periodontitis.
The aim of this study was to investigate the relation between local expression of IL-8 and the localization of neutrophilic granulocytes, using CD16 as a marker of neutrophils. We also investigated the correlation between IL-8 and epithelial proliferation using proliferating cell nuclear antigen (PCNA) as a marker of proliferation. The distribution of IL-8, CD16 and PCNA/cyclin was determined by immunocytochemical techniques. We used cryostat-cut sections from gingival biopsies harvested from 5 subjects with and 5 subjects without periodontitis. Our histological examination demonstrated that the localization of neutrophilic granulocytes in gingival tissue from patients with periodontitis did not correlate with the expression of IL-8. In all tissue sections from patients and controls, the inflammatory cells accumulated near the pocket epithelium and only a few leukocytes deviated from this pattern. In the patient group, keratinocytes not belonging to the pocket or junctional epithelium expressed IL-8 without any evidence of a chemoattractant effect on neutrophils. The marker of proliferation, PCNA/cyclin, was expressed in keratinocytes in the basal cell layer. The expression was less pronounced in the control group. Our finding that IL-8 was expressed in proliferating cells suggests that IL-8 may have a role in keratinocyte proliferation.
Jarnbring
F
F
Department of Periodontology and Center for Oral Biology, Institute of Odontology, Karolinska Institutet, Sweden. Fredrik.Jarnbring@ofa.ki.se
Gustafsson
A
A
Klinge
B
B
D000328:Adult; D000368:Aged; D015415:Biomarkers; D016022:Case-Control Studies; D002455:Cell Division; D005260:Female; D005881:Gingiva / Q000166:cytology / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D016209:Interleukin-8 / Q000096:biosynthesis*; D015603:Keratinocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D020556:Neutrophil Infiltration; D010518:Periodontitis / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis*; D017452:Receptors, IgG
D015415:Biomarkers; D016209:Interleukin-8; D018809:Proliferating Cell Nuclear Antigen; D017452:Receptors, IgG