title: Cloning and sequencing of columbid circovirus (coCV), a new circovirus from pigeons.
abstract: The complete nucleotide sequence of columbid circovirus (CoCV) isolated from pigeons is described. CoCV was amplified using a consensus primer PCR approach directed against conserved sequences within the rep genes of vertebrate circoviruses. The genome of CoCV is circular and 2037 nt in size. It displays 55% homology to the genome of psittacine beak and feather disease virus and is more distantly related (< 40% homology) to porcine circovirus type 1 and 2. Two major open reading frames were identified, encoding the replicase and the putative capsid protein of CoCV. A region similar to the origin of replication of other circoviruses was found: it encompasses a stem-loop structure with the nonamer 5'-TAGTATTAC, conserved in circo-, nano- and geminiviruses. Phylogenetic analyses suggest classification of CoCV as member of the genus Circovirus of the virus family Circoviridae.
authors.affiliation: P24 (Xenotransplantation), Robert Koch-Institut, Berlin, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Mankertz
authors.forename: K
authors.initials: K
authors.lastname: Hattermann
authors.forename: B
authors.initials: B
authors.lastname: Ehlers
authors.forename: D
authors.initials: D
authors.lastname: Soike
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002213:Capsid / Q000235:genetics; D017925:Circovirus / Q000145:classification / Q000235:genetics*; D003001:Cloning, Molecular; D010856:Columbidae / Q000821:virology*; D004265:DNA Helicases / Q000235:genetics; D017931:DNA Primers; D004268:DNA-Binding Proteins; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D016415:Sequence Alignment; D015534:Trans-Activators / Q000235:genetics
chemical_list: D017931:DNA Primers; D004268:DNA-Binding Proteins; D015534:Trans-Activators; C045264:replication initiator protein; D004265:DNA Helicases