Detection of coxsackie B virus infection using a rapid screening method.
A Western blot method (WB) was adapted for rapid screening of antibodies against coxsackie virus B1-B6 in sera from patients with newly diagnosed insulin-dependent diabetes mellitus, myocarditis or febrile syndrome of suspected coxsackie viral aetiology. The use of a mixture of all 6 coxsackie virus B serotypes as the common antigen permitted a very rapid and inexpensive detection of antibody-positive sera for preliminary diagnosis and further detailed assay. Comparison of the results with those obtained in parallel run virus-neutralization tests showed a higher sensitivity and comparable specificity of WB.
Bopegamage
S A
SA
Institute of Preventive and Clinical Medicine, 833 01 Bratislava, Slovakia.
Petrovicová
A
A
Stará
M
M
D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000276:immunology; D015153:Blotting, Western; D003384:Coxsackievirus Infections / Q000097:blood / Q000821:virology*; D029822:Enterovirus B, Human / Q000276:immunology*; D006801:Humans; D009500:Neutralization Tests; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral; D000956:Antigens, Viral