title: A role for the EphA family in the topographic targeting of vomeronasal axons.
abstract: We have investigated the role of the Eph family of receptor tyrosine kinases and their ligands in the establishment of the vomeronasal projection in the mouse. Our data show intriguing differential expression patterns of ephrin-A5 on vomeronasal axons and of EphA6 in the accessory olfactory bulb (AOB), such that axons with high ligand concentration project onto regions of the AOB with high receptor concentration and vice versa. These data suggest a mechanism for development of this projection that is the opposite of the repellent interaction between Eph receptors and ligands observed in other systems. In support of this idea, when given the choice of whether to grow on lanes containing EphA-F(c)/laminin or F(c)/laminin protein (in the stripe assay), vomeronasal axons prefer to grow on EphA-F(c)/laminin. Analysis of ephrin-A5 mutant mice revealed a disturbance of the topographic targeting of vomeronasal axons to the AOB. In summary, these data, which are derived from in vitro and in vivo experiments, indicate an important role of the EphA family in setting up the vomeronasal projection.
authors.affiliation: Department of Physical Biology, Max-Planck-Institute for Developmental Biology, Spemannstrasse 35 / I, 72076 Tübingen, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Knöll
authors.forename: K
authors.initials: K
authors.lastname: Zarbalis
authors.forename: W
authors.initials: W
authors.lastname: Wurst
authors.forename: U
authors.initials: U
authors.lastname: Drescher
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology*; D005314:Embryonic and Fetal Development / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D007797:Laminin / Q000235:genetics / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D009830:Olfactory Bulb / Q000196:embryology*; D009833:Olfactory Pathways / Q000196:embryology*; D020794:Receptor Protein-Tyrosine Kinases / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D036123:Receptor, EphA5; D036141:Receptor, EphA7; D019147:Vomeronasal Organ / Q000166:cytology / Q000196:embryology*; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D007797:Laminin; D020794:Receptor Protein-Tyrosine Kinases; D036123:Receptor, EphA5; D036141:Receptor, EphA7; D001616:beta-Galactosidase