Ca2+-Sensitization of contraction in the h1 calponin-deficient smooth muscle.
Role of h1 calponin on Ca2+-sensitivity of smooth muscle contraction was investigated using h1 calponin gene-deficient mice (CP -/-) and wild type mice (CP +/+). PGF2. induced a comparable force in intact aorta of CP +/+ and CP -/-. DPB showed similar effects to PGF2alpha. In membrane-permeabilized ileal smooth muscle, PDBu enhanced Ca2+-sensitivity of contraction comparably in CP +/+ and CP -/-. GTPgamma-S showed similar effects. Our results suggest that h1 calponin does not regulate Ca2+-sensitivity in the contractile mechanism of smooth muscle.
Yoshimoto
R
R
Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Japan.
Hori
M
M
Takahashi
K
K
Taniguchi
S I
SI
Katsuki
M
M
Ozaki
H
H
Karaki
H
H
D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000502:physiology; D002122:Calcium Chloride / Q000378:metabolism / Q000494:pharmacology*; D002135:Calcium-Binding Proteins / Q000172:deficiency / Q000502:physiology*; D015237:Dinoprost / Q000494:pharmacology; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology; D007082:Ileum; D051379:Mice; D008840:Microfilament Proteins; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology*; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology*; D010120:Oxytocics / Q000494:pharmacology; D010703:Phorbol Esters / Q000494:pharmacology; D000096985:Calponins
D002122:Calcium Chloride; D002135:Calcium-Binding Proteins; D015237:Dinoprost; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D008840:Microfilament Proteins; D010120:Oxytocics; D010703:Phorbol Esters; D000096985:Calponins; C039067:12-deoxyphorbol 13-isobutyrate