The effect of aminoguanidine on urinary heparan sulphate levels in experimental diabetes.
In the present study, we investigated whether the ameliorating effect of aminoguanidine on diabetes-related proteinuria and nephropathy is associated with glomerular basement membrane heparan sulphate contents. STZ-induced diabetic rats developed proteinuria (at the tenth week: diabetic rats, 713 +/- 418 mg protein per millimole creatinine; control rats, <30) and increased urinary heparan sulphate excretion (diabetic rats, 1,400 +/- 83 microg/mmol creatinine; control rats, 41 +/- 13; p < 0.001), suggesting loss of glomerular basement membrane charge. Aminoguanidine treatment of diabetic rats diminished urinary heparan sulphate levels (196 +/- 52), suggesting high incorporation of heparan sulphate-associated charge into glomerular basement membrane. Aminoguanidine administration to diabetic rats also relatively improved proteinuria (456 +/- 255). It is concluded that aminoguanidine treatment has a relative beneficial effect by restoring the diabetes-induced change in renal basement membrane heparan sulphate levels.
Unlüçerçi
Y M
YM
Department of Biochemistry, Istanbul Faculty of Medicine, Istanbul University, Capa, Turkey.
Bekpinar
S
S
Seferoglu
G
G
D000818:Animals; D001485:Basement Membrane / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000652:urine*; D017127:Glycation End Products, Advanced / Q000378:metabolism; D006146:Guanidines / Q000494:pharmacology*; D006497:Heparan Sulfate / Q000652:urine*; D006909:Hydroxyproline / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008297:Male; D011507:Proteinuria / Q000652:urine; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values
D017127:Glycation End Products, Advanced; D006146:Guanidines; D006497:Heparan Sulfate; D006909:Hydroxyproline; C004479:pimagedine