Identification of mouth part antigens of Fasciola gigantica and Toxocara vitulorum and its molecular targets recognized by homologous and heterologous adult anti-sera against adult.
Sodium dodecyl sulphate poly-acrylamide gel electrophoresis (SDS-PAGE) fractionation of whole-worm and mouth part antigens of F. gigantica and T. vitulorum showed obvious qualitative and quantitative differences. Three different anti-sera, raised in rabbits against adult extracts of F. gigantica, T. vitulorum and Monieza expansa, were utilized in immunoblotting for identification of mouth part antigens that cross-react with adult worm of the same species or of different species. 7 and 8 poly peptides were recognized in F. gigantica and T. vitulorum mouth parts respectively by their homologous rabbits anti-adult anti-sera. The cross reactive antigens in F. gigantica which recognized by T. vitulorum anti-sera was 234 KD, while two components of M.wt. 113 and 93 KD were detected by M. expansa serum in the same extract. Furthermore, T. vitulorum antigens which cross-reacted with F. gigantica anti-serum 150 and 65 KD and with M. expansa were 65 and 49 KD.
Hassanain
M A
MA
Department of Parasitology and Animal Diseases, National Research Center, Dokki, Giza, Egypt.
Mahmoud
M S
MS
el-Ezz
N M
NM
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis*; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D005212:Fasciolidae / Q000276:immunology*; D007106:Immune Sera; D008970:Molecular Weight; D009055:Mouth; D014119:Toxocara / Q000276:immunology*
D000947:Antigens, Helminth; D007106:Immune Sera