title: Effects of chronic alcohol consumption on the broad phospholipid signal in human brain: an in vivo 31P MRS study.
abstract: BACKGROUND
  Phosphorus magnetic resonance spectroscopy (31P MRS) allows for the measurement of phospholipids and their breakdown products in the human brain. Fairly mobile membrane phospholipids give rise to a broad signal that co-resonates with metabolic phosphodiesters. Chronic alcohol exposure increases the rigidity of isolated brain membranes and, thus, may affect the amount and transverse relaxation times (T2) of MRS-detectable phospholipids. We tested the hypothesis that subjects who were heavy drinkers have stiffer membranes than controls who were light drinkers, as reflected in a smaller broad signal component and a shorter T2 of the broad signal in 31P MR spectra of the brain.
  
  
  METHODS
  Thirteen alcohol-dependent heavy drinkers (mean age 44 years) were studied by localized 31P MRS in the centrum semiovale and compared with 17 nondependent light drinkers of similar age. The broad component signal was separated from the metabolite signal by convolution difference, which is based on the large difference in line widths of these two signals. Longitudinal and T2 relaxation times were measured using standard methods.
  
  
  RESULTS
  The broad component integral was 13% lower in the brain of heavy drinkers compared with light drinkers (p < 0.001) and remained significantly smaller after corrections for both longitudinal and transverse relaxations (p < 0.01). The T2 distribution of the broad component consistently showed two resolvable components in both groups. The fast relaxing component had the same T2 in both groups (T2 = 1.9 msec). The slower relaxing component T2 was 0.6 msec shorter in heavy drinkers compared with light drinkers (p = 0.08).
  
  
  CONCLUSIONS
  These results, observed in the absence of white matter volume loss, are consistent with biochemical alterations and higher rigidity of white matter phospholipids associated with long-term chronic alcohol abuse. The observed smaller broad signal component in these relatively young heavy drinkers is a sensitive measure of white matter phospholipid damage.
authors.affiliation: Department of Radiology, MR Unit DVA Medical Center, University of California San Francisco, 94121, USA.
authors.forename: M R
authors.initials: MR
authors.lastname: Estilaei
authors.forename: G B
authors.initials: GB
authors.lastname: Matson
authors.forename: G S
authors.initials: GS
authors.lastname: Payne
authors.forename: M O
authors.initials: MO
authors.lastname: Leach
authors.forename: G
authors.initials: G
authors.lastname: Fein
authors.forename: D J
authors.initials: DJ
authors.lastname: Meyerhoff
mesh_terms: D000328:Adult; D000437:Alcoholism / Q000378:metabolism*; D000704:Analysis of Variance; D001921:Brain / Q000378:metabolism*; D005260:Female; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008875:Middle Aged; D010743:Phospholipids / Q000378:metabolism*; D010761:Phosphorus Radioisotopes
chemical_list: D010743:Phospholipids; D010761:Phosphorus Radioisotopes