[Expression of single-chain Fv antibody for anti-beet necrotic yellow vein virus in Escherichia coli].
The heavy chains variable region gene (VH) of monoclonal antibody against beet necrotic yellow vein virus (BNYVV) was amplified from total DNA extracted from anti-BNYVV hybridoma cells by PCR. Sequencing showed that the VH belongs to mouse subgroup II(A) and contains 360 bp, which code one hundred and twenty amino acids. The VH and VL genes were inserted into a plasmid which contains a linker sequence for constructing scFv gene. The new vector named pTC scFv. The scFv was produced in Escherichia coli and appeared binding activity with BNYVV antigen by ELISA method.
Zeng
J Z
JZ
Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China.
Zhou
Z Y
ZY
Wu
Y Q
YQ
Liu
Z P
ZP
Wang
W X
WX
Huang
H L
HL
Cai
Z N
ZN
Yu
J L
JL
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D001483:Base Sequence; D019663:Chenopodiaceae / Q000821:virology*; D004926:Escherichia coli / Q000235:genetics*; D007128:Immunoglobulin Fragments / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D010942:Plant Viruses / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis*
D000911:Antibodies, Monoclonal; D007128:Immunoglobulin Fragments; D011994:Recombinant Proteins; C055966:immunoglobulin Fv