A novel PCR-RFLP assay for the detection of a polymorphism in the 3' of STAT6 gene.
We have identified a novel single nucleotide polymorphism at position 2964 (G/A) in the 3' untranslated region of the human STAT6 gene (GeneBank Accession No. U16031). A novel PCR-RFLP method has been devised for this polymorphism using the amplification-created restriction site (ACRS) method. Allele frequencies in the UK Caucasian population were found to be 0.33 for allele A and 0.67 for allele G.
Amoli
M
M
ARC Epidemiology Unit, Manchester Medical School, Manchester University, Oxford Road, Manchester M13 9PT, UK.
Ollier
W E
WE
Hajeer
A H
AH
D020413:3' Untranslated Regions; D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D005787:Gene Frequency; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D050800:STAT6 Transcription Factor; D015534:Trans-Activators / Q000235:genetics*; D006113:United Kingdom
D020413:3' Untranslated Regions; D017931:DNA Primers; D050800:STAT6 Transcription Factor; C494101:STAT6 protein, human; D015534:Trans-Activators