[Signal transduction and biological function of IL-6 in a human myeloma cell line-XG-7].
OBJECTIVE
To investigate the relationship between IL-6 signal transduction and its biological function on a human IL-6-dependent myeloma cell line-XG-7.


METHODS
Electrophoretic mobility shift (EMSA) and immunopeipitation were used respectively to detect the activation of transcription factors (STAT3 and NF-IL-6) and protein kinases (JAK1 and ERK) in the two IL-6 signal transduction pathways by IL-6 in XG-7 cells. Then the cells were transfected with the sense or anti-sense expression plasmids of the transcription factors, which could up- or down-regulate the activation of the signal transduction pathways specially, the changes of the biological function of IL-6 on XG-7 cells was shown by cell number counting and MTT.


RESULTS
IL-6 can promote the proliferation of XG-7 cells and activate one of the IL-6 signal transduction pathway-Ras/NF-IL-6; but Jak/STAT signal transduction pathway was not activated at the same conditions. When the activation of Ras/NF-IL-6 pathway was up- or down-regulated, the growth effect of IL-6 on XG-7 cells was strongthened or weakened.


CONCLUSIONS
The proliferation of XG-7 cells in the presence of IL-6 is mediated by the activation of Ras/NF-IL-6 signal transduction pathway.
Song
L
L
Institute of Basic Medical Science, Academy of Military Medical Science, Beijing, 100850, China.
Shen
B
B
D002455:Cell Division / Q000187:drug effects; D006801:Humans; D015850:Interleukin-6 / Q000502:physiology*; D009101:Multiple Myeloma / Q000473:pathology / Q000503:physiopathology*; D015398:Signal Transduction / Q000502:physiology*; D014407:Tumor Cells, Cultured
D015850:Interleukin-6