System for cleavable Fc fusion proteins using tobacco etch virus (TEV) protease.
We describe a novel Fc fusion protein system that can be cleaved by tobacco etch virus (TEV) protease. This system is desirable because it takes advantage of the high specificity of TEV protease and its activity at 4 degrees C. We produced two TEV-Fc fusion proteins that contain the first three Ig domains and all six Ig domains of the cell adhesion molecule L1. Both proteins were efficiently cleaved by TEV protease at 4 degrees C. Functional analysis of the cleavage products in neurite outgrowth assays showed they had similar activities to their parental Fc fusion proteins. Therefore, TEV-Fc fusion proteins may increase the utility and flexibility of the Fc fusion protein system.
Haspel
J
J
Rutgers State University of New Jersey, Piscataway, NJ, USA.
Blanco
C
C
Jacob
J
J
Grumet
M
M
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics*; D002460:Cell Line; D004274:DNA, Recombinant; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000378:metabolism*; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000235:genetics / Q000378:metabolism*; D039841:Leukocyte L1 Antigen Complex; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D051379:Mice; D008969:Molecular Sequence Data; D019006:Neural Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D016501:Neurites / Q000187:drug effects / Q000502:physiology; D009841:Oligonucleotides / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology
D004274:DNA, Recombinant; D007141:Immunoglobulin Fc Fragments; D039841:Leukocyte L1 Antigen Complex; D008562:Membrane Glycoproteins; D019006:Neural Cell Adhesion Molecules; D009841:Oligonucleotides; D011993:Recombinant Fusion Proteins; D010450:Endopeptidases; C099488:TEV protease