Additive effect of mPer1 and mPer2 antisense oligonucleotides on light-induced phase shift.
It is well known that light induces both mPer1 and mPer2 mRNA in the suprachiasmatic nucleus. We have reported that mPer1 antisense oligonucleotides (ODNs) inhibited the light-induced phase delays of mouse locomotor rhythm. In this study, we asked whether both or either mPer1 or mPer2 expression is necessary to induce the phase shift. We examined the effects of inhibition of mRNA expression on light-induced phase delays of mouse circadian behavior rhythm. Light-induced phase delays were moderately attenuated by microinjection of mPer1 or mPer2 antisense ODN, but not by mPer3 antisense or mPer1, mPer2 scrambled ODNs, whereas following simultaneous injection of both mPer1 and mPer2 antisense ODNs they disappeared. The present results suggest that acute induction of mPer1 and mPer2 gene play an additive effect on photic entrainment.
Wakamatsu
H
H
Department of Pharmacology and Brain Science, School of Human Sciences, Waseda University, Tokorozawa, Saitama, Japan.
Takahashi
S
S
Moriya
T
T
Inouye
S T
ST
Okamura
H
H
Akiyama
M
M
Shibata
S
S
D000818:Animals; D018797:Cell Cycle Proteins; D002940:Circadian Rhythm / Q000187:drug effects* / Q000502:physiology; D008027:Light; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009043:Motor Activity / Q000187:drug effects* / Q000502:physiology; D009687:Nuclear Proteins / Q000378:metabolism / Q000494:pharmacology*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D056950:Period Circadian Proteins; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D013493:Suprachiasmatic Nucleus / Q000187:drug effects* / Q000378:metabolism; D014157:Transcription Factors
D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; D016376:Oligonucleotides, Antisense; C539022:PER1 protein, human; C539023:Per1 protein, mouse; C513312:Per2 protein, mouse; D056950:Period Circadian Proteins; D012333:RNA, Messenger; D014157:Transcription Factors