Alkyl diol silica: restricted access pre-column packings for fast liquid chromatography-integrated sample preparation of biological fluids.
A silica-based pre-column packed with a 'restricted access material' was used for fully automated solid-phase extraction of drugs and metabolites from untreated biological fluids via a simple valve-switching method. The bifunctional sorbent employed, alkyl diol silica, is characterised by a different chemical modification of the outer surface (diol groups) and the pore surface of the particles (alkyl chains: C4, C8 or C18). This allows for a fast and efficient clean-up of complex biological samples, such as blood, serum, urine or milk, with exclusion of the macromolecular matrix (proteins, nucleic acids) in less than 1.5 min.
Schäfer
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C
Merck KGaA, Darmstadt, Germany. chris.schaefer@merck.de
Lubda
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D000818:Animals; D001322:Autoanalysis; D001774:Blood Chemical Analysis / Q000379:methods; D001826:Body Fluids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D008892:Milk / Q000737:chemistry; D008895:Milk, Human / Q000737:chemistry; D012822:Silicon Dioxide; D014556:Urine / Q000737:chemistry
D012822:Silicon Dioxide