An engineered lox sequence containing part of a long terminal repeat of HIV-1 permits Cre recombinase-mediated DNA excision.
In our previous report, one 34-bp sequence from a long terminal repeat (LTR) of human immunodeficiency virus type 1 (HIV-1) clone, loxLTR-1, was proposed as a target site for site-specific excision by modified Cre recombinase. To support this suggestion, an engineered lox sequence, designated loxIL1, was made. This variant lox has the corresponding sequence of loxLTR-1 at the spacer region and the last two bases of inverted repeat sequence. Through in vitro recombination assay, loxIL1 also allowed the wild-type Cre to specifically recombine the sequence. An in vitro DNA binding experiment with mutants CreK244R and CreK244L revealed that lysine 244 of Cre plays an important role in interaction with the engineered lox. This result suggests that loxLTR-1 would be a candidate for antiviral strategy using site-specific recombinase.
Lee
Y S
YS
Department of Chemistry, Seoul National University, Korea.
Kim
S T
ST
Kim
G W
GW
Lee
M
M
Park
J S
JS
D021901:DNA, Intergenic / Q000235:genetics; D004279:DNA, Viral / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005818:Genetic Engineering; D016325:HIV Long Terminal Repeat / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics*; D019426:Integrases / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D011533:Proviruses / Q000378:metabolism; D011995:Recombination, Genetic; D014764:Viral Proteins
D021901:DNA, Intergenic; D004279:DNA, Viral; D014764:Viral Proteins; C045073:Cre recombinase; D019426:Integrases