title: Localization of the Rh50-like protein to the contractile vacuole in Dictyostelium.
abstract: The human Rhesus (Rh) family consists of three polytopic membrane proteins present at the cell surface of red blood cells. Although Rh proteins are essential for the expression of the blood group system their biological function remains unclear. In this study, the gene encoding a protein homologous to Rh50 in Dictyostelium discoideum was sequenced. The Rh50-like protein was localized to the contractile vacuole, the organelle responsible for maintenance of osmotic equilibrium within the cell. However, Rh50-like-deficient mutants in which the Rh50-like gene was disrupted did not appear to exhibit a phenotype related to osmoregulation. Nevertheless, these mutants may provide a valuable tool for studying the function of the rhesus protein.
authors.affiliation: Université de Genève, Centre Médical Universitaire, Département de Morphologie, Switzerland.
authors.forename: M
authors.initials: M
authors.lastname: Benghezal
authors.forename: D
authors.initials: D
authors.lastname: Gotthardt
authors.forename: S
authors.initials: S
authors.lastname: Cornillon
authors.forename: P
authors.initials: P
authors.lastname: Cosson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001798:Blood Proteins / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D015153:Blotting, Western; D004023:Dictyostelium / Q000737:chemistry* / Q000166:cytology* / Q000235:genetics / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D017353:Gene Deletion; D006023:Glycoproteins / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D006801:Humans; D008562:Membrane Glycoproteins; D008969:Molecular Sequence Data; D009997:Osmotic Pressure; D010641:Phenotype; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014617:Vacuoles / Q000737:chemistry*
chemical_list: D001798:Blood Proteins; D006023:Glycoproteins; D008562:Membrane Glycoproteins; C498523:RHAG protein, human