Induction of nitric oxide synthase activity in adrenal cells.
The induction of nitric oxide synthase (NOS) II by bacterial lypopolysaccharide (LPS) was studied in a steroidogenic mouse tumor adrenal cell line (Y1). Conditioned media from LPS-stimulated peritoneal macrophages induced an increase in NOS II expression as shown by western and northern blot analysis. Accordingly, in the presence of conditioned media an increase in nitrite levels was observed. In addition, steroid production was significantly decreased. In conclusion, NOS II expression could be induced in steroidogenic cells with a concomitant inhibition of steroid production.
Cymeryng
C B
CB
Department of Biochemistry, School of Medicine, University of Buenos Aires, Argentina. cymeryng@fmed.uba.ar
Lotito
S P
SP
Colonna
C
C
Cornejo
M F
MF
Podestá
E J
EJ
D000311:Adrenal Glands / Q000166:cytology / Q000201:enzymology*; D000818:Animals; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II; D009573:Nitrites / Q000378:metabolism; D051381:Rats; D013256:Steroids / Q000037:antagonists & inhibitors / Q000378:metabolism
D017077:Culture Media, Conditioned; D008070:Lipopolysaccharides; D009573:Nitrites; D013256:Steroids; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat