title: The mutagenic potential of AH+ and AH26 by Salmonella/microsome assay.
abstract: The aim of this study was to examine the mutagenic potential of canal sealers AH+ and AH26 by Salmonella/microsome assay. The materials were tested immediately after mixing, 1 hr and 1 month later, respectively. The dimethyl sulfoxide extracts of sealers in amounts of 3.0, 1.5, and 0.75 microliters/plate were used. The plated bacterial strains of Salmonella were TA 98 and TA 100. The results showed that AH+ is mutagenic toward strain TA 100 1 hr after mixing. One month after mixing, mutagenic activity was expressed only in TA 98. Paste A showed strong mutagenicity toward TA 100. AH26 was more mutagenic to the TA 100 immediately after mixing, 1 hr later, and 1 month after it was polymerized. Also it was mutagenic toward TA 98 in the polymerized condition. Further examinations should be conducted to establish a definitive conclusion about mutagenic potential for these two endodontic materials.
authors.affiliation: Department of Dental Pathology, School of Dental Medicine, University of Zagreb, Gundulićeva 5, 10 000 Zagreb, Croatia.
authors.forename: S
authors.initials: S
authors.lastname: Jukić
authors.forename: I
authors.initials: I
authors.lastname: Miletić
authors.forename: I
authors.initials: I
authors.lastname: Anić
authors.forename: S
authors.initials: S
authors.lastname: Britvić
authors.forename: M
authors.initials: M
authors.lastname: Osmak
authors.forename: S
authors.initials: S
authors.lastname: Sistig
mesh_terms: D000818:Animals; D001729:Bismuth / Q000009:adverse effects*; D015169:Colony Count, Microbial; D004121:Dimethyl Sulfoxide; D004338:Drug Combinations; D004853:Epoxy Resins / Q000009:adverse effects*; D008297:Male; D008709:Methenamine / Q000009:adverse effects*; D008862:Microsomes, Liver / Q000187:drug effects*; D016296:Mutagenesis / Q000187:drug effects; D009153:Mutagens / Q000009:adverse effects*; D011108:Polymers / Q000009:adverse effects; D051381:Rats; D017208:Rats, Wistar; D012387:Root Canal Filling Materials / Q000009:adverse effects*; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000254:growth & development; D012834:Silver / Q000009:adverse effects*; D012997:Solvents; D013997:Time Factors; D014025:Titanium / Q000009:adverse effects*
chemical_list: D004338:Drug Combinations; D004853:Epoxy Resins; D009153:Mutagens; D011108:Polymers; D012387:Root Canal Filling Materials; D012997:Solvents; C534916:epoxy resin-based root canal sealer; D012834:Silver; C028044:epoxy resin AH-26; D014025:Titanium; D008709:Methenamine; D001729:Bismuth; D004121:Dimethyl Sulfoxide