title: The chicken stathmin gene and its expression in the embryo.
abstract: Stathmin, which functions as an intracellular relay in signal transduction pathways, has been suggested as a potential indicator of pluripotent cells in the early mouse embryo. In this study, chicken stathmin cDNA and genomic DNA were analyzed. In mammals stathmin consists of five exons and four introns; exons 3, 4, and 5 in the mammalian stathmin gene are equivalent to one relatively large exon in the chicken stathmin gene. Introns equivalent to introns 3 and 4 in the mammalian stathmin gene are not present in the counterpart gene in chickens and, although intron 2 was shown to be present in both mammals and birds, it is smaller in the chicken stathmin gene. Despite differences in the genomic organization of the gene and its smaller size in chickens compared with that in humans and mice, similarities in the coding sequences and in the expression of the chicken and mouse stathmin genes at certain stages of embryo development, as determined by whole-mount in situ hybridization experiments, suggest that their products are functional homologues. The argument is thus substantiated for further investigations into the use of regulatory regions of the stathmin gene in a system for the establishment of long-term cultures of germline competent chicken embryonic stem (ES) cells by the selective ablation of differentiated cells in culture using drug selection.
authors.affiliation: Department of Animal and Poultry Science, University of Guelph, ON, Canada.
authors.forename: S
authors.initials: S
authors.lastname: Soodeen-Karamath
authors.forename: A M
authors.initials: AM
authors.lastname: Gibbins
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002642:Chick Embryo / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015870:Gene Expression; D020869:Gene Expression Profiling; D015698:Genomic Library; D017403:In Situ Hybridization; D051379:Mice; D008868:Microtubule Proteins; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction; D050777:Stathmin
chemical_list: D018076:DNA, Complementary; D008868:Microtubule Proteins; D010750:Phosphoproteins; D050777:Stathmin; C493994:Stmn1 protein, mouse