Electric light scattering from single-stranded DNA in linear polyacrylamide solutions.
The electric light scattering (ELS) of ssDNA (calf thymus, 10 kbp, 55 micrograms/mL) in denaturing polyacrylamide (PAA) solutions was studied as a function of applied sinusoidal electric field and polymer concentration. Electric fields of strengths up to 300 V/cm and of frequencies between 100 and 5000 Hz were applied. It was found that the ELS effect increases with the field strength and decreases at high frequencies. The dependence of the ELS effect of ssDNA on polymer concentration passes through a maximum at 1% PAA. The relaxation times of decay of the ELS effect increase with increasing polymer concentrations. It was demonstrated that ELS is a useful method for investigation of ssDNA behavior in the course of pulse-field electrophoresis in polymer solutions.
Todorov
R
R
Institute of Physical Chemistry, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Starchev
K
K
Stoylov
S P
SP
D000180:Acrylic Resins; D000818:Animals; D002417:Cattle; D004277:DNA, Single-Stranded / Q000032:analysis* / Q000737:chemistry; D004553:Electric Conductivity; D019075:Electrophoresis, Capillary / Q000379:methods; D012542:Scattering, Radiation; D012996:Solutions; D000099166:Polyacrylamides
D000180:Acrylic Resins; D004277:DNA, Single-Stranded; D012996:Solutions; D000099166:Polyacrylamides