title: Identification and characterization of a novel gene, hos2+, the function of which is necessary for growth under high osmotic stress in fission yeast.
abstract: hos2 mutants of the fission yeast Schizosaccharomyces pombe showed the phenotype of high osmolarity sensitivity for growth. An S. pombe strain carrying the hos2-M10 allele cannot form colonies on agar plates containing 2 M glucose, but the parental strain can do so very well, as demonstrated previously. In this study, the hos2+ gene was identified as one that encodes a small protein of 94 amino acids, which shows no sequence similarity to any other proteins in the current databases. The hos2-M10 mutation resulted in Gln-62 to TAG-termination codon. A Hos2-defective (hos2delta) strain, which we then constructed, showed the phenotype of high osmolarity sensitivity, as in the case of the original hos2-M10 mutant. For this hos2delta mutant, three multicopy suppressor genes were isolated and one of which was identified as the pgk1+ gene, encoding a phosphoglycerate kinase.
authors.affiliation: Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Nakamichi
authors.forename: E
authors.initials: E
authors.lastname: Yamamoto
authors.forename: H
authors.initials: H
authors.lastname: Yamada
authors.forename: H
authors.initials: H
authors.lastname: Aiba
authors.forename: T
authors.initials: T
authors.lastname: Mizuno
mesh_terms: D000595:Amino Acid Sequence; D018076:DNA, Complementary / Q000032:analysis; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009997:Osmotic Pressure; D012568:Schizosaccharomyces / Q000235:genetics* / Q000254:growth & development; D029702:Schizosaccharomyces pombe Proteins; D014882:Water-Electrolyte Balance / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D005656:Fungal Proteins; C421189:Hos2 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins