Isogenic transgenic homozygous fish induced by artificial parthenogenesis.
As a model system for vertebrate transgenesis, fish have many attractive advantages, especially with respect to the characteristics of eggs, allowing us to produce isogenic, transgenic, homozygous vertebrates by combining with chromosome-set manipulation. Here, we describe the large-scale production of isogenic transgenic homozygous animals using our experimental organism, the mud loach Misgurnus mizolepis, by the simple process of artificial parthenogenesis in a single generation. These isogenic fish have retained transgenic homozygous status in a stable manner during the subsequent 5 years, and exhibited increased levels of transgene expression. Furthermore, their isogenic nature was confirmed by cloned transgenic homozygous offspring produced via another step of parthenogenic reproduction of the isogenic homozygous transgenic fish. These results demonstrate that a combination of transgenesis and artificial parthenogenesis will make the rapid utilization of genetically pure homozygous transgenic system in vertebrate transgenesis possible.
Nam
Y K
YK
Research Center for Ocean and Industrial Development, Pukyong National University, Pusan, South Korea.
Cho
Y S
YS
Kim
D S
DS
D000818:Animals; D030801:Animals, Genetically Modified; D015139:Blotting, Southern; D003531:Cypriniformes / Q000196:embryology / Q000235:genetics* / Q000502:physiology; D004625:Embryo, Nonmammalian; D005260:Female; D006720:Homozygote; D010312:Parthenogenesis / Q000235:genetics*; D012099:Reproductive Techniques