Sol-gel-derived prussian blue-silicate amperometric glucose biosensor.
A new type of inorganic biosensor is introduced. The sensor comprises glucose oxidase enzymes encapsulated in a sol-gel-derived Prussian blue-silicate hybrid network. Glucose is detected by the biocatalytic reduction of oxygen followed by catalytic reduction of hydrogen peroxide by the Prussian blue catalyst. The sol-gel silicate entails a rigid encapsulating matrix, the Prussian blue provides chemical catalysis and charge mediation from the reduction site to the supporting electrode, and the enzyme is responsible for the biocatalysis. The feasibility of a dual optical/electrochemical mode of analysis is also demonstrated.
Bharathi
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Fredy and Nadine Herrmann School of Applied Sciences, Hebrew University of Jerusalem, Jerusalem, 91904 Israel. ovadia@vms.hvji.ac.il
Lev
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D015374:Biosensing Techniques / Q000295:instrumentation*; D002138:Calibration; D002384:Catalysis; D004396:Coloring Agents / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D004566:Electrodes; D005295:Ferrocyanides / Q000737:chemistry*; D005782:Gels / Q000737:chemistry*; D005947:Glucose / Q000032:analysis; D005949:Glucose Oxidase / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D008956:Models, Chemical; D055095:Optics and Photonics; D017640:Silicates / Q000737:chemistry*; D012984:Software; D013997:Time Factors
D004396:Coloring Agents; D005295:Ferrocyanides; D005782:Gels; D017640:Silicates; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005947:Glucose; C000170:ferric ferrocyanide