Effect of a leukocyte-depleting filter in an extracorporeal circuit used for low-flow ischemia and reperfusion of equine jejunum.
OBJECTIVE
To determine effect of leukocyte depletion on hematologic, morphologic, and metabolic variables of equine jejunum after induction of arterial low-flow ischemia and reperfusion by use of an extracorporeal circuit.


METHODS
14 healthy adult horses.


METHODS
A segment of jejunum was surgically removed and maintained in an isolated circuit for 3 hours (control group), arterial flow was reduced to 20% of baseline for 40 minutes followed by 1 hour of reperfusion (low-flow group), or leukocyte depletion was filter-induced, and low-flow ischemia and reperfusion were conducted as in the low-flow control group (filter-treated group). Various metabolic, hemodynamic, and histomorphologic variables were evaluated, including effects of electrical field stimulation and L-N-nitro-arginine-methyl-ester (L-NAME) on contractile activity.


RESULTS
The extracorporeal circuit appeared to maintain the jejunum within physiologic limits for an extended period. Low-flow ischemia with reperfusion induced significant differences in various measurements, compared with control specimens. Significant differences were not detected between the low-flow and filter-treated groups. Myeloperoxidase activity was greater in the low-flow group than the control group, whereas a difference was not detected between control and filter-treated groups.


CONCLUSIONS
The extracorporeal circuit maintained intestine for 3 hours in a physiologic state and may be used for simulation of tissue injury. Leukocyte depletion generally did not attenuate the effects of low-flow ischemia and reperfusion on equine small intestine.
Van Hoogmoed
L M
LM
Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis 95616, USA.
Snyder
J R
JR
Nieto
J G
JG
Harmon
F A
FA
Timmerman
B L
BL
D000818:Animals; D001783:Blood Flow Velocity; D001794:Blood Pressure; D003106:Colon / Q000166:cytology; D004558:Electric Stimulation; D005112:Extracorporeal Circulation / Q000295:instrumentation / Q000379:methods; D006736:Horses; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000098:blood supply / Q000502:physiology*; D007511:Ischemia / Q000503:physiopathology*; D007583:Jejunum / Q000098:blood supply / Q000187:drug effects / Q000502:physiology*; D007962:Leukocytes / Q000166:cytology / Q000502:physiology*; D008954:Models, Biological; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000098:blood supply / Q000187:drug effects / Q000502:physiology*; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009195:Peroxidase / Q000378:metabolism; D012039:Regional Blood Flow; D015424:Reperfusion
D009195:Peroxidase; D019331:NG-Nitroarginine Methyl Ester