Integrin pattern and effect on contraction in cultured testicular peritubular myoid cells.
OBJECTIVE
Neither the integrin pattern nor the biological functions of integrins have been extensively documented in human cultured testicular peritubular myoid cells (TPMC). The integrin pattern and the presence of some proteins of the immunoglobulin superfamily on human TPMC as well as the role of integrins in TPMC contraction were examined.


METHODS
Integrin expression was evaluated by immunofluorescence and FACS analysis. To assess the role of integrin in TPMC contraction, human and rat cells were added to a collagen gel system and exposed to contractile stimuli.


RESULTS
The immunofluorescence and cytofluorimetric analysis showed that human cultured TPMC express alpha1, alpha2, alpha3, alpha4, alpha5, alpha6, alphav, beta1, beta3, and beta4 integrin subunits, and significant amounts of intercellular adhesion molecule-1 (ICAM-1), whereas they do not present alpha4, beta2, beta7 subunits, nor intercellular adhesion molecule-2 (ICAM-2) and neural cell adhesion molecule (NCAM). The preincubation of human cells with an anti-beta1 mAb and of rat cells with a polyclonal anti-beta1 antibody inhibited TPMC contraction induced by different contractile stimuli.


CONCLUSIONS
Our investigation documented a broad integrin pattern on human cultured TPMC as well as a role for integrins in human and rat TPMC contraction.
Magnanti
M
M
Dipartimento di Medicina Sperimentale e Patologia,  Università La Sapienza, Roma, Italy.
Gismondi
A
A
Gandini
O
O
Rossi
F M
FM
Michetti
P M
PM
Santiemma
V
V
Morrone
S
S
D000328:Adult; D000818:Animals; D002478:Cells, Cultured; D003094:Collagen / Q000502:physiology; D006801:Humans; D016023:Integrins / Q000032:analysis* / Q000502:physiology; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis / Q000502:physiology; D008297:Male; D009119:Muscle Contraction; D051381:Rats; D017207:Rats, Sprague-Dawley; D012671:Seminiferous Tubules / Q000737:chemistry* / Q000166:cytology / Q000502:physiology
D016023:Integrins; D018799:Intercellular Adhesion Molecule-1; D003094:Collagen