[Production of herbicide-resistant rice with transforming heterogene].
Using pAHC20 (containing Bar gene), pWRG1515 (containing GUS gene and hygromycin phosphotransferase gene), and pCAMBIA3300 RG with Bar gene and snowdrop lectin (GNA) gene as donor DNA, the micro-adventitious shoots and the calli induced from mature embryos of Oryza sativa 87203, Eyi105, Shangnong aromatic glutinous rice as recipients were transformed with particle bombardment and Agrobacterium tumefaciens strain LBA4404 containing pAL4404, respectively. After chosen with phosphinothricin and antibiotic, GUS detection and PCR analysis, The results showed that the foreign genes had been transformed microprojectile-mediated to Oryza sativa Eyi105, the regeneration plants were obtained, and, 5 transgenic calli of Oryza sativa Eyi105 were obtained with Agrobacterium-mediated transformation.
Wu
A Z
AZ
Agricultural College of Shanghai Jiaotong University, Shanghai 201101, China.
Tang
K X
KX
Pan
J S
JS
Cai
R
R
Shen
D L
DL
Pan
C G
CG
D017343:Genes, Plant; D006540:Herbicides / Q000494:pharmacology*; D012275:Oryza / Q000235:genetics*; D014170:Transformation, Genetic
D006540:Herbicides