Synthesis and properties of 2'-deoxycytidine triphosphate carrying c-myc tag sequence.
The synthesis of 2'-deoxycytidine triphosphate carrying mercaptoethyl groups at position 4 of cytosine is described. This nucleoside triphosphate was reacted with a maleimido-peptide carrying the c-myc tag-sequence to yield a peptide-nucleoside triphosphate chimera. Primer extension studies showed that the nucleoside triphosphate modified with the peptide sequence is incorporated by DNA polymerases opposite guanine.
Hinz
M
M
Universität Ulm, Sektion Polymere, Germany.
Gottschling
D
D
Eritja
R
R
Seliger
H
H
D000595:Amino Acid Sequence; D001483:Base Sequence; D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D003845:Deoxycytosine Nucleotides / Q000138:chemical synthesis* / Q000737:chemistry* / Q000378:metabolism; D004795:Enzyme Stability; D016259:Genes, myc
D017931:DNA Primers; D003845:Deoxycytosine Nucleotides; C024107:2'-deoxycytidine 5'-triphosphate; D004259:DNA-Directed DNA Polymerase