Development of a computer software for analysis of SDS-PAGE protein fingerprints of bacterial isolates.
Protein fingerprinting is a widely used technique in epidemiological studies for typing bacterial strains. This study reports the development of a computer based gel analysis system. The system has the capability to analyse SDS-PAGE whole-cell protein profiles using digital image processing techniques. The software incorporates spatial and frequency domain operators for image enhancement, support for geometric correction of images and new algorithms for identification of strain tracks and protein bands. The system also provides facilities for correcting imaging defects for inter-gel comparison, similarity analysis, clustering and pictorial representation of results as a dendrogram. The software is highly interactive, user-friendly and can produce accurate results for differentiation of bacterial strains with minimal overhead of time.
Saxena
S K
SK
Department of Electrical Engineering, Indian Institute of Technology, New Delhi, 110 016.
Ibrahim
A N
AN
Chaudhury
S
S
Thukral
S S
SS
D000465:Algorithms; D001419:Bacteria / Q000737:chemistry* / Q000145:classification*; D001426:Bacterial Proteins / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D004812:Epidemiologic Methods; D006801:Humans; D007711:Klebsiella pneumoniae / Q000737:chemistry / Q000302:isolation & purification; D010449:Peptide Mapping / Q000379:methods* / Q000706:statistics & numerical data; D012967:Sodium Dodecyl Sulfate; D012984:Software
D001426:Bacterial Proteins; D012967:Sodium Dodecyl Sulfate