Micelle-mediated extraction and preconcentration of ginsenosides from Chinese herbal medicine.
The feasibility of employing micelle-mediated extraction as an alternative and effective method for the solubilization, purification and/or preconcentration of active ingredients from herbal products is demonstrated for the first time using the root of American ginseng as a model. When compared to methanol and water, an aqueous surfactant solution containing 10% Triton X-100 yielded faster kinetics and higher recovery for the extraction of various ginsenosides. An experimental design approach (uniform design) was demonstrated as a novel and useful method for the optimization of experimental factors involved in the micelle-mediated extraction process. For the preconcentration of ginsenosides prior to chromatographic determination, a salting-out agent (sodium sulfate) was employed to make the efficient cloud point extraction of both hydrophobic and hydrophilic ginsenosides into the surfactant-rich phase possible, as well as to increase the preconcentration factor by reducing the volume of the surfactant-rich phase.
Fang
Q
Q
Department of Chemistry, Hong Kong Baptist University, Kowloon Tong.
Yeung
H W
HW
Leung
H W
HW
Huie
C W
CW
D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D036145:Ginsenosides; D008823:Micelles; D012503:Saponins / Q000032:analysis / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; D008823:Micelles; D012503:Saponins