title: Influence of fatty alcohol and other fatty acid derivatives on fatty acid uptake into rat intestinal epithelial cells.
abstract: We investigated the influence of various substrates on the uptake of long-chain fatty acid into IEC-6, rat intestinal epithelial cell line. The uptake of [3H]oleic acid into IEC-6 cells was a saturable function of the oleic acid concentration. Long-chain fatty acids significantly inhibited the oleic acid uptake into IEC-6 cells and shorter-chain fatty acids had little or no effect. Various fatty acid esters suppressed the oleic acid uptake into IEC-6. Fatty alcohols also inhibited oleic acid uptake into IEC-6 and the length of the carbon chain played an important role. These results suggest that long-chain fatty acid uptake was inhibited by the substrates which had a structure similar to long-chain fatty acids, especially those with a long carbon chain. At least two molecules, fatty acid translocase and fatty acid transport protein type 4, which are considered to be involved in the long-chain fatty acid transport into the cell, were expressed on IEC-6 cells, supporting the existence of the carrier-mediated system of long-chain fatty acid transport on IEC-6 cells.
authors.affiliation: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Murota
authors.forename: N
authors.initials: N
authors.lastname: Matsui
authors.forename: T
authors.initials: T
authors.lastname: Kawada
authors.forename: N
authors.initials: N
authors.lastname: Takahashi
authors.forename: T
authors.initials: T
authors.lastname: Shintani
authors.forename: K
authors.initials: K
authors.lastname: Sasaki
authors.forename: T
authors.initials: T
authors.lastname: Fushiki
mesh_terms: D000818:Animals; D018955:CD36 Antigens; D002352:Carrier Proteins / Q000235:genetics; D002460:Cell Line; D004847:Epithelial Cells / Q000737:chemistry / Q000378:metabolism; D050612:Fatty Acid Transport Proteins; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D005233:Fatty Alcohols / Q000737:chemistry / Q000494:pharmacology*; D015870:Gene Expression; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000737:chemistry; D008562:Membrane Glycoproteins / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics; D026901:Membrane Transport Proteins; D019301:Oleic Acid / Q000378:metabolism; D027361:Organic Anion Transporters; D012313:RNA / Q000032:analysis; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013329:Structure-Activity Relationship; D014316:Tritium
chemical_list: D018955:CD36 Antigens; D002352:Carrier Proteins; D050612:Fatty Acid Transport Proteins; D005227:Fatty Acids; D005233:Fatty Alcohols; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D027361:Organic Anion Transporters; D014316:Tritium; D019301:Oleic Acid; D012313:RNA