Influence of dehydroepiandrosterone on platelet aggregation, superoxide dismutase activity and serum lipid peroxide concentrations in rabbits with induced hypercholesterolemia.
Special interest in the role of DHEA dates back to the finding of a correlation between low serum DHEA concentrations and a higher morbidity and mortality rate due to coronary diseases in humans. Animal studies with experimental atherosclerosis confirmed the anti-sclerotic effect of DHEA. The mechanism of DHEA action remains unclear. We determined the influence of dehydroepiandrosterone (DHEA) administration, a potential anti-atherogenic agent, on platelet aggregation, platelet superoxide dismutase (SOD) activity and serum lipid peroxide (LPO) levels in male rabbits fed on a normal and atherogenic diet. 44 adult male New Zealand white rabbits were divided into 4 groups: 1--control group fed on standard rabbit food, 2--fed on an atherogenic diet, 3--fed on an atherogenic diet with DHEA, 4--fed on standard food with DHEA. We detected blood platelet aggregation following (ADP) and collagen activation by means of photometry. Platelet SOD activity was detected by means of fluorometry determining the inhibition of adrenaline auto-oxidation. The serum LPO concentration was measured by means of the colorimetric method. The serum DHEA-S concentration was measured by means of RIA methods, and serum lipid levels were measured by means of Biomérieux manufactured kits. Results demonstrate that (1) elevated LPO concentrations in rabbits with hyperlipidemia did not decrease following DHEA administration. (2) In rabbits fed on a normal diet, DHEA caused a decrease of LPO, which emphasizes the positive influence of this steroid on the oxidative stress in healthy animals. Such a result was not seen in the group with severe hyperlipidemia. (3) Rabbits with hyperlipidemia demonstrated a significantly decreased SOD activity. (4) In healthy animals as well as in those with hyperlipidemia, DHEA administration caused an increase of platelet SOD activity, the main enzyme of the antioxidant defence system, which protects the organism against free radical damage (5) (DHEA had no influence on platelets aggregation in both tested groups).


CONCLUSIONS
DHEA administration improves platelet SOD activity, which protects cells against oxidative damage. The hypothesis that DHEA administration leads to an increase in antioxidant potency requires further investigations.
Bednarek-Tupikowska
G
G
Department of Endocrinology and Diabetology, Medical University, ul. Pasteura 4, 50-367 Wrocław, Poland.
Gosk
I
I
Szuba
A
A
Bohdanowicz-Pawlak
A
A
Kosowska
B
B
Bidzińska
B
B
Milewicz
A
A
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001161:Arteriosclerosis / Q000517:prevention & control; D001792:Blood Platelets / Q000187:drug effects / Q000201:enzymology; D003094:Collagen / Q000494:pharmacology; D003687:Dehydroepiandrosterone / Q000494:pharmacology*; D019314:Dehydroepiandrosterone Sulfate / Q000097:blood; D006937:Hypercholesterolemia / Q000097:blood* / Q000188:drug therapy*; D066298:In Vitro Techniques; D008054:Lipid Peroxides / Q000097:blood*; D008297:Male; D010974:Platelet Aggregation / Q000187:drug effects*; D011817:Rabbits; D013482:Superoxide Dismutase / Q000097:blood*
D000975:Antioxidants; D008054:Lipid Peroxides; D003687:Dehydroepiandrosterone; D019314:Dehydroepiandrosterone Sulfate; D000244:Adenosine Diphosphate; D003094:Collagen; D013482:Superoxide Dismutase