title: In addition to the high-density lipoprotein fraction, apolipoprotein C-III is detected in chylomicrons and the very low-density lipoprotein fraction from serum of normolipidemic cows.
abstract: Apolipoprotein (apo) C-III is a low-molecular-mass protein that is involved in the regulation of the triglyceride metabolism. Except for the hyperlipidemic calf, cattle apoC-III is mainly detected in the high-density lipoprotein (HDL) fraction, and the distribution in chylomicrons (CM) and the very low-density lipoprotein (VLDL) fraction has not yet been clarified. The purpose of the present study was to detect apoC-III in concentrated CM and VLDL fractions to examine whether apoC-III is distributed in the two fractions even in normolipidemic cattle. ApoC-III could be detected by immunoblot analysis in both concentrated cow CM and VLDL fractions, but not in the corresponding calf fractions. These results suggest that apoC-III is distributed in the CM and VLDL fractions, at least in cows, although the concentrations in these fractions are considerably lower than in the HDL fraction.
authors.affiliation: Hokkaido Research Station, National Institute of Animal Health, Sapporo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Katoh
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D053304:Apolipoprotein C-II; D001056:Apolipoproteins C / Q000097:blood*; D002417:Cattle / Q000097:blood*; D002914:Chylomicrons / Q000097:blood*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D015151:Immunoblotting; D007774:Lactation; D008075:Lipoproteins, HDL / Q000097:blood*; D008079:Lipoproteins, VLDL / Q000097:blood*; D008297:Male; D012016:Reference Values
chemical_list: D053304:Apolipoprotein C-II; D001056:Apolipoproteins C; D002914:Chylomicrons; D008075:Lipoproteins, HDL; D008079:Lipoproteins, VLDL