title: Homogenous implant in rat tibias of matrix preserved in 98% glycerin: histomorphologic study.
abstract: As a chemical medium for preservation of tissues, glycerin has shown good results because it maintains the cellular integrity despite the tissue dehydration it causes. Taking advantage of the osteoinducing properties of the osseous matrix and glycerin as a proper medium for tissue preservation, osseous matrix was implanted in rat tibias. Twenty-four rats were used, each receiving two surgical wounds. In one of the wounds an osseous matrix preserved in 98% glycerin was implanted and the other received a matrix without preservatives. Six animals were sacrificed on days 10, 20, 30 and 60 post-implant. After routine histological processing, the specimens were stained in hematoxylin-eosin and Masson's trichrome. The results showed that the matrixes preserved in glycerin presented faster resorption with replacement by newly formed tissue.
authors.affiliation: Disciplina de Cirurgia e Traumatologia Buco-Maxilo-Facial, Faculdade de Odontologia, UNESP, Araçatuba, SP, Brasil.
authors.forename: T
authors.initials: T
authors.lastname: Okamoto
authors.forename: M E
authors.initials: ME
authors.lastname: Kira
authors.forename: R
authors.initials: R
authors.lastname: Okamoto
authors.forename: I R
authors.initials: IR
authors.lastname: Garcia Júnior
authors.forename: W D
authors.initials: WD
authors.lastname: Niccoli Filho
mesh_terms: D000818:Animals; D001391:Azo Compounds; D001857:Bone Matrix / Q000473:pathology / Q000637:transplantation*; D001862:Bone Resorption / Q000473:pathology; D003094:Collagen; D004396:Coloring Agents; D003238:Connective Tissue / Q000473:pathology; D004801:Eosine Yellowish-(YS); D005347:Fibroblasts / Q000473:pathology; D005456:Fluorescent Dyes; D005500:Follow-Up Studies; D005990:Glycerol / Q000627:therapeutic use*; D006416:Hematoxylin; D008297:Male; D008739:Methyl Green; D010006:Osteoblasts / Q000473:pathology; D010012:Osteogenesis / Q000502:physiology; D011310:Preservatives, Pharmaceutical / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D013977:Tibia / Q000601:surgery; D014021:Tissue Preservation; D014184:Transplantation, Homologous
chemical_list: D001391:Azo Compounds; D004396:Coloring Agents; D005456:Fluorescent Dyes; D011310:Preservatives, Pharmaceutical; C017768:trichrome stain; D008739:Methyl Green; D003094:Collagen; D005990:Glycerol; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin