Antigens of monoclonal antibody NB3C4 are novel markers for oligodendrocytes.
We produced NB3C4, a novel monoclonal antibody specific for oligodendrocytes, using human neuroblastoma IMR-32 cells. NB3C4 specifically recognized oligodendrocytes in the CNS, although it bound to neuroblastoma IMR-32 cells and oligodendrocytes in vitro. Double immunofluorescence staining of rat brain using NB3C4 and anti-GST-pi, anti-glial fibrillary acidic protein (GFAP), or anti-neurofilament 200 (NF) antibody revealed that anti-GST-pi antibody identified an oligodendrocyte marker recognizing NB3C4-positive cells, while both anti-GFAP and anti-NF antibody did not. Western blotting of rat brain homogenates showed that NB3C4 bound three proteins of 22-28 kDa, while the anti-GST-pi recognized a 27 kDa protein. Therefore, antigens recognized by NB3C4 could be novel markers for oligodendrocytes.
Yoshimura
K
K
Department of Physiology, Saitama Medical School, Iruma-gun, Japan.
Kametani
F
F
Shimoda
Y
Y
Fujimaki
K
K
Sakurai
Y
Y
Kitamura
K
K
Asou
H
H
Nomura
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D000918:Antibody Specificity; D015415:Biomarkers; D001921:Brain / Q000166:cytology; D005260:Female; D006801:Humans; D007114:Immunization; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009447:Neuroblastoma; D009836:Oligodendroglia / Q000276:immunology*; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D015415:Biomarkers