Development of a chemiluminescent optical fiber immunosensor to detect Streptococcus pneumoniae antipolysaccharide antibodies.
A chemiluminescent-based optical fiber immunosensor was developed for the detection of antipneumococcal antibodies. This was accomplished by developing a different chemical procedure utilizing 3-aminopropyl trimethoxysilane and cyanuric chloride to conjugate pneumococcal cell wall polysaccharides to the optical fiber tips, and by improving the sensitivity of the photodetection system. The lowest titer of antipneumococcal antibodies detected by the optical fiber was at a 1:819,200 dilution. The lowest corresponding value by standard enzyme-linked immunosorbent assay was at a 1:98,415 dilution. It was concluded that the optical immunosensor system is an accurate and sensitive method to detect antipneumococcal antibodies and may be an adequate tool to monitor antibodies in specimens such as saliva and urine.
Marks
R S
RS
Unit of Biotechnology, Soroka University, Beer-Sheva, Israel. Rsmarks@bgumail.bgu.ac.il
Margalit
A
A
Bychenko
A
A
Bassis
E
E
Porat
N
N
Dagan
R
R
D000906:Antibodies / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation*; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D005336:Fiber Optic Technology; D007118:Immunoassay; D008163:Luminescent Measurements; D008956:Models, Chemical; D055100:Optical Fibers; D011134:Polysaccharides / Q000737:chemistry* / Q000276:immunology; D011437:Propylamines / Q000737:chemistry; D012463:Saliva / Q000737:chemistry; D012680:Sensitivity and Specificity; D012821:Silanes / Q000737:chemistry; D013296:Streptococcus pneumoniae / Q000276:immunology*; D014227:Triazines / Q000737:chemistry; D014556:Urine / Q000737:chemistry
D000906:Antibodies; D011134:Polysaccharides; D011437:Propylamines; D012821:Silanes; D014227:Triazines; C088294:3-aminopropyltrimethoxysilane; C019309:cyanuric chloride