title: Characterization of proteases in the skin mucus of Atlantic salmon (Salmo salar) infected with the salmon louse (Lepeophtheirus salmonis) and in whole-body louse homogenate.
abstract: As part of an investigation of the biochemical interactions between the salmon louse Lepeophtheirus salmonis and Atlantic salmon Salmo salar, we characterized protease activity in the skin mucus of noninfected Atlantic salmon and Atlantic salmon infected with L. salmonis and in an L. salmonis whole-body homogenate. Zymography revealed that mucus from infected salmon contained a series of low-molecular-mass (17-22 kDa) serine proteases that were not present in the mucus of noninfected salmon. Based on molecular mass, inhibition studies, and affinity chromatography, the series of proteases was identified as being trypsin-like. Similar proteases were observed in the L. salmonis homogenate and in mucus from noninfected Atlantic salmon following a 1-hr incubation with live L. salmonis. An antibody raised against Atlantic salmon trypsin failed to recognize any proteases in the mucus of noninfected salmon or infected salmon or in the L. salmonis homogenate. Collectively, these findings suggest that the trypsin-like proteases present in the mucus of infected Atlantic salmon were produced by L. salmonis, possibly to aid in feeding and evasion of host immune responses.
authors.affiliation: Institute for Marine Biosciences, National Research Council of Canada, Halifax, Nova Scotia.
authors.forename: K J
authors.initials: KJ
authors.lastname: Firth
authors.forename: S C
authors.initials: SC
authors.lastname: Johnson
authors.forename: N W
authors.initials: NW
authors.lastname: Ross
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D003445:Crustacea / Q000201:enzymology*; D004478:Ectoparasitic Infestations / Q000201:enzymology / Q000469:parasitology / Q000662:veterinary*; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D010450:Endopeptidases / Q000737:chemistry*; D005393:Fish Diseases / Q000201:enzymology* / Q000469:parasitology; D006790:Host-Parasite Interactions; D008970:Molecular Weight; D009093:Mucus / Q000201:enzymology*; D011480:Protease Inhibitors / Q000494:pharmacology; D020317:Salmo salar / Q000469:parasitology*; D012867:Skin / Q000201:enzymology / Q000469:parasitology
chemical_list: D011480:Protease Inhibitors; D010450:Endopeptidases