title: A fluorescent-labeled oligopeptide for monitoring PKA-mediated phosphorylation.
abstract: A fluorescent-labeled oligopeptide (DACM-CLRRASLK-fluorescein), containing a consensus amino acid sequence (RRXSL) of cyclic AMP (cAMP) dependent protein kinase A (PKA) substrate-proteins, was designed. The fluorescent peptide was a good substrate of PKA, and the phosphorylation of its serin residue caused an intensive change in fluorescent intensity. We expect that the peptide will be useful as a fluorescent indicator for monitoring PKA activity in living cells.
authors.affiliation: Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Ohuchi
authors.forename: Y
authors.initials: Y
authors.lastname: Katayama
authors.forename: M
authors.initials: M
authors.lastname: Maeda
mesh_terms: D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000302:isolation & purification*; D009842:Oligopeptides / Q000302:isolation & purification*; D010766:Phosphorylation; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D009842:Oligopeptides; D017868:Cyclic AMP-Dependent Protein Kinases