title: Continuous separation of green fluorescent protein by annular chromatography.
abstract: The concept of annular chromatography was tested by separation of a real protein solution used in biotechnology. Green fluorescent protein was expressed in S. cerevisiae and the extract was continuously separated by a pressurized annular chromatograph packed with a Superdex 200 prep grade size-exclusion chromatography medium. Purity was checked by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Western blotting and fluorescent intensity. The continuous mode was compared to batchwise operation. Under the assumption that equivalent fractions are collected, both modes are comparable.
authors.affiliation: Institute of Applied Microbiology, University of Agricultural Sciences, Vienna, Austria.
authors.forename: A
authors.initials: A
authors.lastname: Uretschläger
authors.forename: A
authors.initials: A
authors.lastname: Einhauer
authors.forename: A
authors.initials: A
authors.lastname: Jungbauer
mesh_terms: D002852:Chromatography, Ion Exchange / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000302:isolation & purification
chemical_list: D008164:Luminescent Proteins; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins