title: Comparative FISH mapping of the ancestral fusion point of human chromosome 2.
abstract: It is known that human chromosome 2 originated from the fusion of two ancestral primate chromosomes. This has been confirmed by chromosome banding and fluorescence in-situ hybridization (FISH) with human chromosome-2-specific DNA libraries. In this study, the order of 38 cosmid clones derived from the human chromosome region 2q12-q14 was exactly determined by high-resolution FISH in human chromosome 2 and its homologous chromosomes in chimpanzees (Pan trogrodydes, 2n=48) and cynomolgus monkeys (Macacafascicularis, 2n = 42). This region includes the telomere-to-telomere fusion point of two ancestral ape-type chromosomes. As a result of comparative mapping, human chromosome region 2q12-q14 was found to correspond to the short arms of chimpanzee chromosomes 12 and 13 and cynomolgus monkey chromosomes 9 and 15. It is noted that no difference was detected in the relative order of the cosmid clones between human and chimpanzee chromosomes. This suggests that two ancestral ape-type chromosomes fused tandemly at telomeres to form human chromosome 2, and the genomic organization of this region is thought to be considerably conserved. In the cynomolgus monkey, however, the order of clones in each homologue was inverted. In addition to cosmid mapping, two chromosome-2-specific yeast artificial chromosome (YAC) clones containing the fusion point were identified by FISH.
authors.affiliation: Department of Biological Sciences, Graduate School of Science, University of Tokyo, Japan.
authors.forename: F
authors.initials: F
authors.lastname: Kasai
authors.forename: E
authors.initials: E
authors.lastname: Takahashi
authors.forename: K
authors.initials: K
authors.lastname: Koyama
authors.forename: K
authors.initials: K
authors.lastname: Terao
authors.forename: Y
authors.initials: Y
authors.lastname: Suto
authors.forename: K
authors.initials: K
authors.lastname: Tokunaga
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: M
authors.initials: M
authors.lastname: Hirai
mesh_terms: D000818:Animals; D007446:Chromosome Inversion; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics*; D002999:Clone Cells; D003360:Cosmids / Q000235:genetics; D019143:Evolution, Molecular; D015723:Gene Library; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008252:Macaca fascicularis / Q000235:genetics; D002679:Pan troglodytes / Q000235:genetics; D020161:Physical Chromosome Mapping / Q000379:methods*; D017422:Sequence Analysis, DNA; D016615:Telomere / Q000235:genetics