Functional analysis of the PsbX protein by deletion of the corresponding gene in Synechocystis sp. PCC 6803.
The psbX gene (sml0002) coding for a 4.1 kDa protein in Photosystem II of plants and cyanobacteria was deleted in both wild type and in a Photosystem I-less mutant of the cyanobacterium Synechocystis sp. PCC 6803. Polymerase chain reaction and sequencing analysis showed that the mutants had completely segregated. Deletion of the PsbX protein does not seem to influence growth rate, electron transport or water oxidation ability. Whereas a high light induction of the psbX mRNA could be observed in wild type, deletion of the gene did not lead to high light sensibility. Light saturation measurements and 77K fluorescence measurements indicated a minor disconnection of the antenna in the deletion mutant. Furthermore, fluorescence induction measurements as well as immuno-staining of the D1 protein showed that the amount of Photosystem II complexes in the mutants was reduced by 30%. Therefore, PsbX does not seem to be necessary for the Photosystem II electron transport, but directly or indirectly involved in the regulation of the amount of functionally active Photosystem II centres in Synechocystis sp. PCC 6803.
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Department of Biochemistry, Stockholm University, Sweden. christiane@biokemi.su.se
D020418:Algal Proteins / Q000235:genetics / Q000502:physiology; D015152:Blotting, Northern; D000458:Cyanobacteria / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D017353:Gene Deletion; D015151:Immunoblotting; D008027:Light; D009154:Mutation; D010100:Oxygen / Q000378:metabolism; D010777:Photochemistry; D010788:Photosynthesis / Q000235:genetics / Q000502:physiology; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics* / Q000378:metabolism; D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D012313:RNA / Q000235:genetics / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D014158:Transcription, Genetic
D020418:Algal Proteins; D045322:Photosynthetic Reaction Center Complex Proteins; D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; C113691:photosystem II, PSII-X subunit; D012313:RNA; D010100:Oxygen