title: Involvement of CD1 in peripheral deletion of T lymphocytes is independent of NK T cells.
abstract: During peripheral T cell deletion, lymphocytes accumulate in nonlymphoid organs including the liver, a tissue that expresses the nonclassical, MHC-like molecule, CD1. Injection of anti-CD3 Ab results in T cell activation, which in normal mice is followed by peripheral T cell deletion. However, in CD1-deficient mice, the deletion of the activated T cells from the lymph nodes was impaired. This defect in peripheral T cell deletion was accompanied by attenuated accumulation of CD8(+) T cells in the liver. In tetra-parental bone marrow chimeras, expression of CD1 on the T cells themselves was not required for T cell deletion, suggesting a role for CD1 on other cells with which the T cells interact. We tested whether this role was dependent on the Ag receptor-invariant, CD1-reactive subset of NK T cells using two other mutant mouse lines that lack most NK T cells, due to deletion of the genes encoding either beta(2)-microglobulin or the TCR element J alpha 281. However, these mice had no abnormality of peripheral T cell deletion. These findings indicate a novel role for CD1 in T cell deletion, and show that CD1 functions in this process through mechanisms that does not involve the major, TCR-invariant set of NK T cells.
authors.affiliation: Immunobiology Section, Yale University School of Medicine, New Haven, CT 06510, USA.
authors.forename: T
authors.initials: T
authors.lastname: Dao
authors.forename: M
authors.initials: M
authors.lastname: Exley
authors.forename: W Z
authors.initials: WZ
authors.lastname: Mehal
authors.forename: S M
authors.initials: SM
authors.lastname: Tahir
authors.forename: S
authors.initials: S
authors.lastname: Snapper
authors.forename: M
authors.initials: M
authors.lastname: Taniguchi
authors.forename: S P
authors.initials: SP
authors.lastname: Balk
authors.forename: I N
authors.initials: IN
authors.lastname: Crispe
mesh_terms: D000818:Animals; D018949:Antigens, CD1 / Q000235:genetics / Q000502:physiology*; D017252:CD3 Complex / Q000276:immunology; D016516:CD4-CD8 Ratio; D016923:Cell Death / Q000276:immunology; D002465:Cell Movement / Q000276:immunology; D003602:Cytotoxicity, Immunologic / Q000235:genetics; D015536:Down-Regulation / Q000276:immunology; D007106:Immune Sera / Q000008:administration & dosage; D007274:Injections, Intraperitoneal; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D008099:Liver / Q000276:immunology / Q000473:pathology; D008213:Lymphocyte Activation / Q000235:genetics; D008231:Lymphopenia / Q000235:genetics / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D011948:Receptors, Antigen, T-Cell / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology* / Q000378:metabolism / Q000473:pathology
chemical_list: D018949:Antigens, CD1; D017252:CD3 Complex; D007106:Immune Sera; D011948:Receptors, Antigen, T-Cell