[Use of in situ hybridization and reverse transcriptase polymerase chain reaction in situ methods for studying infection caused by the hepatitis C virus].
Low level of hepatitis C virus (HCV) replication in hepatocytes does not provide reliable morphological or ultrastructural criteria for detecting an HCV infection by optic and electron microscopy. On the other hand, detection of cellular and subcellular location and tropism of HCV and comparison of virus replication in infected tissues with the level of viremia in patients with hepatitis C require modern methods, combining morphological and molecular biological aspects of diagnosis, such as in situ hybridization (ISH) and reverse transcription polymerase chain reaction in situ (RT-PCR in situ). The target for ISH is HCV RNA, while for RT-PCR in situ it is amplified HCV cDNA, which notably improves the sensitivity of hybridization.
Severova
A A
AA
Manykin
A A
AA
D006526:Hepatitis C / Q000175:diagnosis*; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
Primenenie metodov gibridizatsii in situ i obratnotranskriptaznoĭ polimeraznoĭ tsepnoĭ reaktsii in situ dlia izucheniia infektsii, vyzvannoĭ virusom gepatita C.