Biosensor for determination of glucose and sucrose in fruit juices by flow injection analysis.
Glucose and sucrose were measured with an amperometric method by using the flow injection analysis technique. A carbon paste electrode with a renewable surface containing glucose oxidase, horseradish peroxidase, and ferrocene was used in combination with the soluble enzymes invertase and mutarotase. The effect of invertase, mutarotase, and ascorbic acid on the electrode response was examined. Glucose and sucrose concentrations were determined with < 3% errors. The proposed method for glucose and sucrose measurements was validated in real samples of fruit juices. The results were also compared with those obtained with the ultraviolet method.
Guémas
Y
Y
Groupe Electrochimie-Laboratoire d'Analyse Isotopique et Electrochimique de Métabolismes (UPRSA-CNRS 6006), Faculté des Sciences et des Techniques, 2, rue de la Houssinière, B.P. 92208 44322 Nantes, France.
Boujtita
M
M
el Murr
N
N
D001205:Ascorbic Acid / Q000737:chemistry; D001628:Beverages / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation*; D002138:Calibration; D002238:Carbohydrate Epimerases / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D005296:Ferrous Compounds / Q000737:chemistry; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry; D006026:Glycoside Hydrolases / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000737:chemistry; D000075163:Metallocenes; D008956:Models, Chemical; D013395:Sucrose / Q000032:analysis*; D013997:Time Factors; D014466:Ultraviolet Rays; D043324:beta-Fructofuranosidase
D005296:Ferrous Compounds; D000075163:Metallocenes; D013395:Sucrose; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase; D002238:Carbohydrate Epimerases; C019646:aldose 1-epimerase; D005947:Glucose; D001205:Ascorbic Acid; C004998:ferrocene