Possible effects of antioxidant status on increased platelet aggregation in childhood iron-deficiency anemia.
BACKGROUND
Alterations in platelet function and antioxidant status in children with iron-deficiency anemia (IDA) have been reported previously. The present study was performed to better understand possible interactions between these two systems.


METHODS
Erythrocyte superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activity and platelet function were evaluated in 15 children (aged 1 1/2-15 years) with IDA. The antioxidant enzyme activity was determined spectrophotometrically. Platelet aggregation and secretion studies were performed using impedance and bioluminescence methods, respectively. Ten age-matched healthy children were included as a control group.


RESULTS
There were no differences in SOD and CAT activities between patients and controls. However, GSH-Px activity was significantly lower in the iron-deficient children. Platelet aggregation responses to collagen and ADP were also significantly higher in iron-deficient children than in controls.


CONCLUSIONS
Decreased antioxidant defense in IDA may cause increased oxidant stress, which, in turn, may result in a tendency towards platelet aggregation.
Tekin
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D
Department of Physiology, Ankara University School of Medicine, Turkey. demet_tekin@hotmail.com
Yavuzer
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S
Tekin
M
M
Akar
N
N
Cin
S
S
D000293:Adolescent; D018798:Anemia, Iron-Deficiency / Q000097:blood / Q000201:enzymology / Q000378:metabolism*; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D007223:Infant; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D010974:Platelet Aggregation / Q000502:physiology*; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase