Detection of a mixed-backbone oligonucleotide (GEM 231) in liver and tumor tissues by capillary electrophoresis.
A simple, rapid and sensitive method has been developed and validated for the analysis of a mixed-backbone oligonucleotide (GEM 231) in tumor tissues. The analysis was performed using a capillary electrophoresis (CE) system with UV detection. An extended light path (bubble cell) capillary column of 64.5 cm (effective length 56 cm) x 50 microm I.D. is used as the separation column. The optimized chromatographic conditions were background electrolyte: sodium borate buffer (60 mM, pH 9.1), electrokinetic injection: 10 s, applied voltage: 30 kV, detection at lambda = 210 nm. A linear relationship was observed between the peak area and the amount of GEM 231 in the range of 1.0-1000 microg/ml. The lower detection limit of the drug was 100 pg with an average recovery of about 75 +/- 5%. The inter-day and intra-day relative standard deviations were <10%. Assay validation studies revealed that CE method is reproducible and specific for the determination of GEM 231 in tissue homogenates with a run time of less than 5 min.
Bansal
R
R
Department of Pharmacology, Georgetown University Medical Center, Washington, DC 20007, USA.
Chen
H X
HX
Marshall
J L
JL
Tan
J
J
Glazer
R I
RI
Wainer
I W
IW
D000818:Animals; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D008099:Liver / Q000737:chemistry*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000737:chemistry*; D009841:Oligonucleotides / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
C425328:GEM231; D009841:Oligonucleotides