Comparison of the column performance of narrow-bore and standard-bore columns for the chromatographic determination of alpha-, beta-, gamma-, and delta-tocopherol.
A comparison of the performance of narrow-bore (2.1-mm i.d.) and standard-bore (4.6-mm i.d.) analytical silica columns having the same length is completed for the resolution of alpha-, beta-, gamma-, and delta-tocopherol. The studies are performed on high-performance liquid chromatographic equipment with minimum extracolumn contribution. Column permeabilities are 1.16 x 10(-9) and 2.48 x 10(-9) cm2 for narrow and standard bore, respectively. The narrow-bore column gives up to a 7 times increase in sensitivity compared with a standard-bore column at equivalent running times for the analytes. Approximately one-third solvent savings can be achieved with the narrow-bore column. Theoretical plates of the standard-bore column are higher than that of the narrow-bore column.
Ye
L
L
Department of Food Science and Technology, University of Georgia, Athens 30602, USA.
Landen
W O
WO
Eitenmiller
R R
RR
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D007536:Isomerism; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D014810:Vitamin E / Q000032:analysis* / Q000737:chemistry
D014810:Vitamin E