title: Plasma protein carbonyls in nonpregnant, healthy pregnant and preeclamptic women.
abstract: Increased reactive oxygen species (ROS) and lipid peroxidation may be implicated in the pathogenesis of preeclampsia by causing cell (membrane) damage and impaired endothelial function. Carbonyl derivatives of proteins, or protein carbonyls, may be sensitive biomarkers of ROS-mediated damage. The aim of the study was to compare levels of protein carbonyls in plasma of preeclamptic, healthy pregnant and healthy nonpregnant women. Plasma protein carbonyls were measured in 47 preeclamptic, 45 healthy pregnant and 22 healthy nonpregnant women by using a sensitive ELISA-method. ANOVA, the unpaired t-test and Pearson's correlation were used for statistical analysis. Preeclamptic women had significantly higher plasma protein carbonyl levels than healthy pregnant women (P < 0.0001). Healthy pregnant women showed significantly higher protein carbonyl levels (P < 0.001) as compared to nonpregnant controls. The higher levels of protein carbonyls as compared to nonpregnant controls suggest that increased oxygen free radical damage occurs in normal pregnancy and to a much higher extent in preeclampsia.
authors.affiliation: Department of Obstetrics and Gynaecology, University Hospital Nijmegen, The Netherlands.
authors.forename: P L
authors.initials: PL
authors.lastname: Zusterzeel
authors.forename: T P
authors.initials: TP
authors.lastname: Mulder
authors.forename: W H
authors.initials: WH
authors.lastname: Peters
authors.forename: S A
authors.initials: SA
authors.lastname: Wiseman
authors.forename: E A
authors.initials: EA
authors.lastname: Steegers
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D017359:HELLP Syndrome / Q000097:blood; D006801:Humans; D015227:Lipid Peroxidation; D011225:Pre-Eclampsia / Q000097:blood*; D011247:Pregnancy; D011506:Proteins / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012720:Severity of Illness Index
chemical_list: D015415:Biomarkers; D011506:Proteins; D017382:Reactive Oxygen Species