Constitutive expression of soybean ferritin cDNA in transgenic wheat and rice results in increased iron levels in vegetative tissues but not in seeds.
We used particle bombardment to produce transgenic wheat and rice plants expressing recombinant soybean ferritin, a protein that can store large amounts of iron. The cDNA sequence was isolated from soybean by RT-PCR and expressed using the constitutive maize ubiquitin-1 promoter. The presence of ferritin mRNA and protein was confirmed in the vegetative tissues and seeds of transgenic wheat and rice plants by northern and western blot analysis, respectively. The levels of ferritin mRNA were similar in the vegetative tissues of both species, but ferritin protein levels were higher in rice. Both ferritin mRNA and protein levels were lower in wheat and rice seeds. ICAP spectrometry showed that iron levels increased only in vegetative tissues of transgenic plants, and not in the seeds. These data indicate that recombinant ferritin expression under the control of the maize ubiquitin promoter significantly increases iron levels in vegetative tissues, but that the levels of recombinant ferritin in seeds are not sufficient to increase iron levels significantly over those in the seeds of non-transgenic plants.
Drakakaki
G
G
Molecular Biotechnology Unit, John Innes Centre, Norwich, UK.
Christou
P
P
Stöger
E
E
D018076:DNA, Complementary; D005293:Ferritins / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D007501:Iron / Q000378:metabolism*; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D012639:Seeds / Q000378:metabolism; D030262:Soybean Proteins / Q000235:genetics*; D014908:Triticum / Q000235:genetics* / Q000378:metabolism; D014452:Ubiquitins / Q000235:genetics
D018076:DNA, Complementary; D030262:Soybean Proteins; D014452:Ubiquitins; D005293:Ferritins; D007501:Iron