Intragastric CO2 and nitric oxide participate in the regulation of peptone-induced gastrin release in humans.
BACKGROUND
Moderate acidification of the gastric lumen inhibits peptone-induced gastrin release. The aim of the present study was to investigate if the gastric acid neutralization products CO2 (from secreted HCO3) and NO (from reduced salivary nitrite) could act as intermediate messengers between luminal acidity and the inhibition of peptone-induced gastrin release.


METHODS
Fourteen healthy volunteers (mean age, 27 years; range, 20-39 years; 3 women) participated in the study. Intragastric perfusion with saline or peptone was performed on the healthy volunteers. Venous blood samples were analyzed for serum gastrin concentrations. Intragastric NO was measured by chemiluminescence.


RESULTS
Basal serum gastrin ranged between 11 and 23 pmol/l. Peptone in Sörensen's phosphated buffer (pH 6.9, PCO2 0 mmHg) increased serum gastrin by 83% +/- 23%, whereas acidified peptone (pH 2.0) did not stimulate gastrin release. Acidified peptone buffered with NaHCO3 to neutrality (pH 6.9, PCO2 approximately 600 mmHg) increased serum gastrin by 166% +/- 29%. Low intragastric NO levels were obtained by deviation of saliva. During such salivary depletion, acidified peptone (pH 2.0) stimulated gastrin release to a level of about 40% of the control response (pH 6.9). This peptone-induced gastrin response during salivary deviation was inhibited by addition of nitrite to the perfusate.


CONCLUSIONS
Acid-induced inhibition of peptone-stimulated gastrin release is partly dependent on intraluminal NO formed in the reaction between salivary nitrite and gastric acid. In addition, the gastric acid neutralization product CO2 seems to potentiate the effect of peptone on gastrin release.
Holm
M
M
Dept of Physiology, Göteborg University, Sweden.
Olbe
L
L
Fändriks
L
L
D000328:Adult; D002245:Carbon Dioxide / Q000378:metabolism / Q000494:pharmacology*; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism*; D005755:Gastrins / Q000097:blood* / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism / Q000494:pharmacology*; D010461:Peptones / Q000494:pharmacology; D012463:Saliva / Q000378:metabolism
D005755:Gastrins; D010461:Peptones; D002245:Carbon Dioxide; D009569:Nitric Oxide