title: Apoptosis and mitosis of parenchymal cells in the duct-ligated rat submandibular gland.
abstract: Apoptosis and proliferation of parenchymal cells during atrophy of rat submandibular gland induced by double duct ligation were investigated using immunohistochemistry for proliferating cell nuclear antigen (PCNA), terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-digoxigenin nick end labelling (TUNEL) and transmission electron microscopy (TEM). At 2 and 3 days after ligation, increased PCNA positive cells and mitoses were seen in ducts; thereafter PCNA positive cells decreased in number. At 3 and 4 days, the acinar cell population rapidly decreased, with many remaining TUNEL positive acinar cells. During this period, TEM showed typical apoptotic acinar cells that were phagocytosed by adjacent acinar cells or intraepithelial macrophages. After 7 days, most acinar cells had disappeared, leaving prominent residual ducts; a few acinar cells remained, especially at the lobule periphery. Submandibular gland duct ligation thus induced marked depletion of acinar cell by apoptosis and a concurrent short-lived cycle of duct cell proliferation.
authors.affiliation: Department of Oral Health Sciences, Hokkaido University Graduate School of Dentistry, Kita 13, Nishi 7, Kita-ku, Sapporo, 060-0813, Japan. tshigeru@den.hokudai.ac.jp
authors.forename: S
authors.initials: S
authors.lastname: Takahashi
authors.forename: S
authors.initials: S
authors.lastname: Nakamura
authors.forename: R
authors.initials: R
authors.lastname: Suzuki
authors.forename: N
authors.initials: N
authors.lastname: Islam
authors.forename: T
authors.initials: T
authors.lastname: Domon
authors.forename: T
authors.initials: T
authors.lastname: Yamamoto
authors.forename: M
authors.initials: M
authors.lastname: Wakita
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001284:Atrophy; D020287:In Situ Nick-End Labeling; D008026:Ligation; D008297:Male; D008854:Microscopy, Electron; D008938:Mitosis / Q000502:physiology*; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D018987:Salivary Ducts; D022142:Secretory Vesicles / Q000473:pathology; D013363:Submandibular Gland / Q000737:chemistry / Q000473:pathology* / Q000648:ultrastructure
chemical_list: D018809:Proliferating Cell Nuclear Antigen