In vitro assays of vesicular transport.
Movement of proteins and lipids between the various compartments of eukaryotic cells is fundamental to the maintenance of cellular homeostasis, and an understanding of the molecular mechanisms that govern these processes remains a key goal of cell biological research. This aim has been greatly facilitated by the development of assays that recapitulate specific events in vitro. In the following article we provide an overview of some of the currently used assays that measure the movement of proteins within the exocytic and endocytic pathways, and provide a starting point for those wishing to establish their own systems to study other vesicular transport steps.
Cook
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NR
Wellcome Trust Centre for Molecular Mechanisms in Disease, University of Cambridge, Wellcome Trust/MRC Building, Addenbrookes Hospital, Hills Road, Cambridge, CB2 2XY, UK.
Davidson
H W
HW
D000818:Animals; D001671:Biochemistry / Q000379:methods; D002021:Buffers; D002458:Cell Fractionation; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000737:chemistry / Q000378:metabolism; D008561:Membrane Fusion; D021381:Protein Transport; D022161:Transport Vesicles / Q000737:chemistry / Q000378:metabolism*
D002021:Buffers