Globosal basal cells are identified as proliferating cells in mouse olfactory epithelium.
Although anti-bromodeoxyuridine (BrdU) antibodies are used for detecting proliferating cells, they may stain proliferating cells in phases following the S-phase, except for G1. Anti-Ki67 antibodies are widely used for detecting proliferating cells in all phases of the cell cycle (G1-, S-, G2-, and M-phase), but not in resting cells (G0-phase). Anti-cyclin D1 antibodies are used to detect proliferating cells in the G1-phase. The present study investigated the olfactory epithelium of mice by double immunostaining using antibodies to BrdU, Ki67. cyclin D1, and cytokeratin 14 (CK14). The cells positive to the anti-BrdU antibody, the anti-Ki67 antibody, and the anti-cyclin D1 antibody differed from the cells positive to the anti-CK14 antibody. Thus, we confirmed that the proliferating cells in all the phases of the cell cycle, including the G1-phase, were globosal basal cells, which were the precursors of the olfactory cells.
Ohta
Y
Y
Department of Otolaryngology-Head and Neck Surgery, Jichi Medical School, Minamikawachi, Tochigi, Japan.
Ichimura
K
K
D000818:Animals; D000906:Antibodies; D001973:Bromodeoxyuridine / Q000032:analysis; D002453:Cell Cycle; D002455:Cell Division; D019938:Cyclin D1 / Q000032:analysis; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis; D019394:Ki-67 Antigen / Q000032:analysis; D051379:Mice; D009831:Olfactory Mucosa / Q000166:cytology*
D000906:Antibodies; D019394:Ki-67 Antigen; D019938:Cyclin D1; D007633:Keratins; D001973:Bromodeoxyuridine