title: The protein-protein interaction map of Helicobacter pylori.
abstract: With the availability of complete DNA sequences for many prokaryotic and eukaryotic genomes, and soon for the human genome itself, it is important to develop reliable proteome-wide approaches for a better understanding of protein function. As elementary constituents of cellular protein complexes and pathways, protein-protein interactions are key determinants of protein function. Here we have built a large-scale protein-protein interaction map of the human gastric pathogen Helicobacter pylori. We have used a high-throughput strategy of the yeast two-hybrid assay to screen 261 H. pylori proteins against a highly complex library of genome-encoded polypeptides. Over 1,200 interactions were identified between H. pylori proteins, connecting 46.6% of the proteome. The determination of a reliability score for every single protein-protein interaction and the identification of the actual interacting domains permitted the assignment of unannotated proteins to biological pathways.
authors.affiliation: Hybrigenics SA, Paris, France.
authors.forename: J C
authors.initials: JC
authors.lastname: Rain
authors.forename: L
authors.initials: L
authors.lastname: Selig
authors.forename: H
authors.initials: H
authors.lastname: De Reuse
authors.forename: V
authors.initials: V
authors.lastname: Battaglia
authors.forename: C
authors.initials: C
authors.lastname: Reverdy
authors.forename: S
authors.initials: S
authors.lastname: Simon
authors.forename: G
authors.initials: G
authors.lastname: Lenzen
authors.forename: F
authors.initials: F
authors.lastname: Petel
authors.forename: J
authors.initials: J
authors.lastname: Wojcik
authors.forename: V
authors.initials: V
authors.lastname: Schächter
authors.forename: Y
authors.initials: Y
authors.lastname: Chemama
authors.forename: A
authors.initials: A
authors.lastname: Labigne
authors.forename: P
authors.initials: P
authors.lastname: Legrain
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000378:metabolism*; D001665:Binding Sites; D016208:Databases, Factual; D004926:Escherichia coli / Q000235:genetics; D015723:Gene Library; D016480:Helicobacter pylori / Q000378:metabolism*; D006801:Humans; D020407:Internet; D008969:Molecular Sequence Data; D011485:Protein Binding; D020543:Proteome; D016415:Sequence Alignment; D012984:Software; D014510:Urease / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; D014510:Urease