Spermine increases the active and passive transport across the alveolar epithelium in situ: effect of thiol reagents.
An intact alveolar epithelial barrier is thought to be important for alveolar liquid absorption. However, polycations increase alveolar permeability without affecting alveolar liquid absorption (Saumon et al., Am J Physiol 1995: 269:L185-L194). We have reconsidered this issue using polyamines. The polyamine spermine (10(-3) mol/l) produced a large (up to 20-fold), sustained increase in the permeability of the alveolar barrier to mannitol (PAMan) and in alveolar liquid absorption (Jw, twofold) in isolated rat lungs. These increases were inhibited by 5 x 10(-3) mol/l putrescine and 2 x 10(-3) mol/l spermidine. Because spermine is known to affect the phosphoinositide/Ca2+ signalling pathway, we evaluated the effects of thiol reagents known to interfere with this pathway in different ways. Thimerosal, a thiol reagent which sensitizes the inositol 1,4,5-trisphosphate (IP3) receptor, inhibited the spermine-induced increase in PA(Man) and, to a lesser extent, that of Jw. Mersalyl, a thiol reagent which blocks IP3-gated Ca2+ channels, enhanced spermine's effect, whereas N-ethylmaleimide, a non-specific thiol reagent, had no effect. These observations show that large increases in permeability may coexist with increases in Jw. They also suggest that the phosphoinositide/Ca2+ second messenger pathway is involved in modulating the tightness of the alveolar barrier and alveolar liquid absorption.
Saumon
G
G
INSERM U82, Faculté Xavier Bichat, Paris, France. saumon@bichat.inserm.fr
Martet
G
G
D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D001693:Biological Transport, Active / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000494:pharmacology; D015220:Calcium Channels / Q000187:drug effects / Q000502:physiology; D002463:Cell Membrane Permeability / Q000187:drug effects; D005033:Ethylmaleimide / Q000494:pharmacology; D007202:Indicators and Reagents; D015544:Inositol 1,4,5-Trisphosphate / Q000494:pharmacology; D053496:Inositol 1,4,5-Trisphosphate Receptors; D015640:Ion Channel Gating / Q000187:drug effects; D008353:Mannitol / Q000378:metabolism; D008634:Mersalyl / Q000494:pharmacology; D010716:Phosphatidylinositols / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000187:drug effects* / Q000378:metabolism*; D011700:Putrescine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D018160:Receptors, Cytoplasmic and Nuclear / Q000187:drug effects / Q000502:physiology; D015290:Second Messenger Systems; D013095:Spermidine / Q000494:pharmacology; D013096:Spermine / Q000494:pharmacology*; D013438:Sulfhydryl Compounds / Q000494:pharmacology*; D013849:Thimerosal / Q000494:pharmacology
D002121:Calcium Channel Blockers; D015220:Calcium Channels; D007202:Indicators and Reagents; D053496:Inositol 1,4,5-Trisphosphate Receptors; D010716:Phosphatidylinositols; D018160:Receptors, Cytoplasmic and Nuclear; D013438:Sulfhydryl Compounds; D013849:Thimerosal; D013096:Spermine; D008353:Mannitol; D008634:Mersalyl; D000584:Amiloride; D015544:Inositol 1,4,5-Trisphosphate; D005033:Ethylmaleimide; D002118:Calcium; D013095:Spermidine; D011700:Putrescine