title: [Clinical utility of transcription mediated amplification-hybridization protection method(TMA-HPA) for the detection of HBV DNA: a comparison with the branched DNA probe assay].
abstract: The clinical utility of the TMA-HPA method for the detection of HBV DNA was evaluated by comparing results with the branched DNA probe(b-DNA) assay. Sera from 135 biopsy-confirmed HBV carriers were included in the study. Dilution tests with two representative sera revealed the sensitivity of the TMA-HPA method to be 100-fold higher than that of the b-DNA assay. Among the 135 test sera, HBV DNA measurements obtained by the two assays showed a high degree of correlation(r = 0.861, p < 0.0001). Of the 72 sera which tested positive for HBe antigen, 71 and 67 were positive for HBV DNA when tested by TMA-HPA and b-DNA assay, respectively. In contrast, of the 63 which tested negative for HBe antigen, 42 were positive for HBV DNA when tested by TMA-HPA, and only 19 were positive when tested by b-DNA assay(p < 0.05). Among HBe antigen-positive HBV carriers, HBV DNA levels as determined both TMA-HPA and b-DNA assay showed an inverse relationship with pathological stage, but among HBe antigen-negative HBV carriers, no such tendency was observed. Based on these results, it is felt that the detection of HBV DNA by TMA-HPA is useful for the evaluating anti-viral therapeutic effect in HBV carriers, and in estimating a prognosis.
authors.affiliation: Hepatitis Research Institute, Suginami-ku, Tokyo 167-0033.
authors.forename: H
authors.initials: H
authors.lastname: Hoshino
authors.forename: K
authors.initials: K
authors.lastname: Hino
authors.forename: S
authors.initials: S
authors.lastname: Sainokami
authors.forename: H
authors.initials: H
authors.lastname: Miyakawa
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D021121:Branched DNA Signal Amplification Assay; D002353:Carrier State / Q000175:diagnosis*; D015342:DNA Probes; D004279:DNA, Viral / Q000097:blood*; D005260:Female; D006509:Hepatitis B / Q000175:diagnosis*; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D021141:Nucleic Acid Amplification Techniques; D009693:Nucleic Acid Hybridization / Q000379:methods*; D014158:Transcription, Genetic
chemical_list: D015415:Biomarkers; D015342:DNA Probes; D004279:DNA, Viral