title: Quantitative analysis of mucin and lectin in maxillary sinus fluids in patients with acute and chronic sinusitis.
abstract: OBJECTIVE
  Sinusitis is characterized by quantitative and qualitative changes in mucus biosynthesis that contribute to sinus disease. In general, patients with acute sinusitis complain of purulent rhinorrhea, whereas those with chronic sinusitis complain of mucoid or mucopurulent rhinorrhea Locally produced mucin largely contributes to the high viscoelasticity of mucus in sinusitis. In this study, the authors attempt to quantify the concentrations of mucin and lectin in the maxillary sinus fluids from these patients.
  
  
  METHODS
  To assess the concentrations of mucin and lectin in aspirates of 11 acutely and 11 chronically inflamed maxillary sinuses, the concentration of mucin was measured by enzyme-linked immunosorbent assay (ELISA) and that of lectin by sandwiched enzyme-linked lectin assay (ELLA).
  
  
  RESULTS
  The concentrations of mucin and lectin that were measured using monoclonal antibodies (HCS 4, HCS 14, and HCS 18) and Ulex europaeus agglutinin-1 (UEA-1) in chronic sinusitis were higher than those in acute sinusitis. The concentration of total protein in chronic sinusitis was lower than that in acute sinusitis. Each mucin and lectin versus total protein ratio in chronic sinusitis was higher than that in acute sinusitis.
  
  
  CONCLUSIONS
  These data suggest that hypersecretion in chronic sinusitis may result from locally increased mucin production. Comprehension of this mechanism may be a strategy to prevent the viscous cycle of paranasal sinus fluids in chronic sinusitis.
authors.affiliation: Department of Otorhinolaryngology, Mie University School of Medicine, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Shinogi
authors.forename: T
authors.initials: T
authors.lastname: Harada
authors.forename: T
authors.initials: T
authors.lastname: Nonoyama
authors.forename: C
authors.initials: C
authors.lastname: Kishioka
authors.forename: Y
authors.initials: Y
authors.lastname: Sakakura
authors.forename: Y
authors.initials: Y
authors.lastname: Majima
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D001424:Bacterial Infections / Q000175:diagnosis*; D002908:Chronic Disease; D005260:Female; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D008297:Male; D015523:Maxillary Sinusitis / Q000175:diagnosis*; D008875:Middle Aged; D009077:Mucins / Q000378:metabolism*
chemical_list: D037102:Lectins; D009077:Mucins