Cytotoxic effect of methyl methacrylate on 4 cultured fibroblasts.
OBJECTIVE
The objective of this study was to examine the cytotoxic effect of methyl methacrylate (MMA) monomer on 4 mammalian fibroblasts.


METHODS
Four cells--C3H10T1/2, L929, Balb/3T3 clone A31, and MC3T3-E1--were incubated for 6 days in 24-well microplates filled with 1 mL of Dulbecco's Modified Eagle medium containing MMA monomer that ranged from 0 to 200 mM/L. Cytotoxicity was evaluated by direct cell number count.


RESULTS
L929 possessed the largest TC50 value (the amount of MMA that caused 50% cell death) (34 mM/L), and Balb/3T3 clone A31 had the least value (1 mM/L); C3H10T1/2 and MC3T3-E1 had intermediate values (25 mM/L and 16 mM/L, respectively).


CONCLUSIONS
This study showed that the MMA monomer had dose-dependent cytotoxicity, which varied among the cells tested.
Taira
M
M
Department of Dental Materials Science and Technology, Iwate Medical University School of Dentistry, 1-3-27 Chuo-dori, Morioka 020-8505, Japan. mtaira@iwate-med.ac.jp
Nakao
H
H
Matsumoto
T
T
Takahashi
J
J
D016475:3T3 Cells / Q000187:drug effects; D000818:Animals; D002452:Cell Count; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects*; D007739:L Cells / Q000187:drug effects; D007928:Lethal Dose 50; D020366:Methylmethacrylate / Q000633:toxicity*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H
D020366:Methylmethacrylate