Factors influencing the cellular location of proteolytic enzymes of Bacillus intermedius.
Thiol-dependent serine proteinase and glutamylendopeptidase of Bacillus intermedius 3-19 being prevailing enzymes in the total pool of extracellular proteinases (95%) of this microorganism in catalytic active form were detected on the membrane of the cells. Production of these enzymes was maximum on the medium containing inorganic phosphate and gelatin and decreased 2-4-fold on the medium with glucose and lactate. The level of the activity of extracellular enzymes correlated with that of corresponding membrane-bound proteins. The addition of CoCl2 (2 mM) into the medium caused essential increase in extracellular glutamylendopeptidase activity and promoted the release of membrane-bound enzyme into cultural fluid. Proteolytic activity was detected in cytoplasm also. Proteinases localized in cytoplasm were shown to differ in properties from those secreted.
Sharipova
M R
MR
Department of Microbiology, Kazan State University, Kremlievskaya str, 18, Kazan 420008, Russia. Margarita.Sharipova@ksu.ru
Shakirov
E V
EV
Gabdrakhmanova
L A
LA
Balaban
N P
NP
Kalacheva
N V
NV
Rudenskaya
G N
GN
Leshchinskaya
I B
IB
D001407:Bacillus / Q000201:enzymology* / Q000254:growth & development; D002364:Caseins; D002462:Cell Membrane / Q000201:enzymology; D003470:Culture Media; D003593:Cytoplasm / Q000201:enzymology; D010450:Endopeptidases / Q000302:isolation & purification / Q000378:metabolism*; D011523:Protoplasts / Q000201:enzymology; D012697:Serine Endopeptidases / Q000302:isolation & purification / Q000378:metabolism; D013379:Substrate Specificity
D002364:Caseins; D003470:Culture Media; D010450:Endopeptidases; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase