title: Spleen antibacterial peptides: high levels of PR-39 and presence of two forms of NK-lysin.
abstract: Antibacterial peptides were isolated from porcine spleen by acetic acid extraction, ion exchange chromatography and reverse-phase high-performance liquid chromatography. C-terminal ladder sequence analysis of a bioactive peptide with matrix-assisted laser desorption/ionization mass spectrometry after digestion with carboxypeptidases P and Y showed that it is identical to the antibacterial proline/arginine-rich intestinal peptide PR-39. It is present at high levels in granulocytes of the spleen, and peptides with C-terminal proline amide and internal adjacent Pro residues can be analyzed with this method. In addition, two forms of NK-lysin (NKL) were found. One, NKLi, is identical to that isolated from pig intestine, and the other, NKLbw, to a mature peptide deduced from a clone from a porcine bone marrow cDNA library.
authors.affiliation: Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
authors.forename: V
authors.initials: V
authors.lastname: Bonetto
authors.forename: M
authors.initials: M
authors.lastname: Andersson
authors.forename: T
authors.initials: T
authors.lastname: Bergman
authors.forename: R
authors.initials: R
authors.lastname: Sillard
authors.forename: A
authors.initials: A
authors.lastname: Norberg
authors.forename: V
authors.initials: V
authors.lastname: Mutt
authors.forename: H
authors.initials: H
authors.lastname: Jornvall
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry; D023181:Antimicrobial Cationic Peptides / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004797:Enzyme-Linked Immunosorbent Assay; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D011510:Proteolipids / Q000737:chemistry; D011663:Pulmonary Surfactants / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013154:Spleen / Q000378:metabolism*; D013552:Swine
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; C093238:NK-lysin; D010455:Peptides; D011510:Proteolipids; D011663:Pulmonary Surfactants; C072212:PR 39