Effect of hemoglobin E on glycosylated hemoglobin determinations using different commercial kits.
The effect of HbE, a hemoglobin variant, on the determination of HbA1/HbA1c using 4 commercial kits based on cation-exchange resin, cation-exchange column chromatography and specific antibody techniques was studied. Fifty-eight normal and 63 HbE heterozygous subjects were tested for HbA1 and HbA1c using 4 commercial kits i.e. Eagles Diagnostics, Boehringer Mannehim (BM), Diastat and Ames DCA 2000. Analyses of the samples by the 4 kits were done within one week and samples were stored at 4 degrees C before analysis. The results showed that HbE affects the determination of glycosylated hemoglobin using cation-exchange based and not kits based on specific antibody techniques.
Musalmah
M
M
Department of Chemical Pathology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan.
Normah
J
J
Mohamad
W B
WB
Salwah
O N
ON
Fatah
H A
HA
Zahari
N A
NA
D000293:Adolescent; D000328:Adult; D002852:Chromatography, Ion Exchange; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis*; D006446:Hemoglobin E / Q000032:analysis*; D006801:Humans; D007158:Immunologic Techniques; D008297:Male; D008875:Middle Aged; D011933:Reagent Kits, Diagnostic
D006442:Glycated Hemoglobin; D006446:Hemoglobin E; D011933:Reagent Kits, Diagnostic