title: Purification and characterization of a lectin from the mushroom Mycoleptodonoides aitchisonii.
abstract: A lectin was isolated from the mushroom Mycoleptodonoides aitchisonii by means of affinity chromatography on bovine submaxillary mucin (BSM)-Toyopearl and gel filtration on Superose 12 HR10/30 using a FPLC system. This lectin is composed of four identical 16 kDa subunits and the molecular mass of the intact lectin was estimated to be 64 kDa by gel filtration. In a hemagglutination inhibition assay, it exhibited strong sugar-binding specificity towards asialo-BSM among the mono- or oligo-saccharides and glycoproteins tested. The binding specificity of the lectin was also examined by surface plasmon resonance analysis.
authors.affiliation: Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Japan. achkawa@agr.shizuoka.ac.jp
authors.forename: H
authors.initials: H
authors.lastname: Kawagishi
authors.forename: J
authors.initials: J
authors.lastname: Takagi
authors.forename: T
authors.initials: T
authors.lastname: Taira
authors.forename: T
authors.initials: T
authors.lastname: Murata
authors.forename: T
authors.initials: T
authors.lastname: Usui
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000596:Amino Acids / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification*; D020349:Surface Plasmon Resonance
chemical_list: D000596:Amino Acids; D037102:Lectins