Risk of infection by reprocessed and resterilized virus-contaminated catheters; an in-vitro study.
OBJECTIVE
In spite of increasing reuse of disposable catheters, there are few scientific data on potential viral transmission and infection after reuse. To determine the theoretical risk of virus transmission during reuse of catheters an in vitro study was performed using an RNA virus (echovirus-11) and a DNA virus (adenovirus-2).


RESULTS
After deliberate contamination of the catheters, reprocessing and reuse of the cleaned and glutaraldehyde sterilized catheters was simulated. The presence of residual virus was determined by cell culture and by polymerase chain reaction (PCR). After the sterilization step, infectious enterovirus was detectable in one (10%) of the samples, whereas two (20%) contained detectable enterovirus RNA. After simulated reuse, enterovirus was cultured from one (10%) of the catheters, but no less than six (60%) of the samples were enterovirus PCR positive and one (10%) contained detectable adenovirus DNA. After sonification of the catheter tips no infectious virus could be detected, but enterovirus RNA was detected in two (20%) and adenovirus DNA in three (30%) of the samples.


CONCLUSIONS
It has been clearly demonstrated in this in vitro study that, even after rigorous cleaning and sterilization, virus was still present in the catheter. Reuse of catheters, labelled for single-use only, is dangerous and should be prevented.
Luijt
D S
DS
Regional Public Health Laboratory, Groningen, The Netherlands.
Schirm
J
J
Savelkoul
P H
PH
Hoekstra
A
A
D002404:Catheterization / Q000295:instrumentation*; D018929:Cell Culture Techniques / Q000379:methods; D003428:Cross Infection / Q000635:transmission* / Q000821:virology*; D004267:DNA Viruses / Q000302:isolation & purification; D004209:Disposable Equipment; D004866:Equipment Contamination; D017743:Equipment Reuse; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D012328:RNA Viruses / Q000302:isolation & purification; D012307:Risk Factors; D013242:Sterilization; D014777:Virus Diseases / Q000635:transmission*