[Studies on metabolite of beta-elemene in rat bile].
OBJECTIVE
To study the metabolite of beta-elemene in the bile of rat.


METHODS
After i.v. 100 mg.kg-1 beta-elemene, the metabolite in rat bile was extracted by ether. Mass spectrometry, nuclear magnetic resonance, infrared spectrometry and ultraviolet spectrometry were used to analyze the metabolite of i.v. beta-elemene in rat bile.


RESULTS
It was confirmed by nuclear magnetic resonance that the methyl of No. 11 carbon connecting with No. 10 carbon of a metabolite in rat bile was oxidized to be aldehyde. The molecular weight of the metabolite was 218 which detected by mass spectrometry. Infrared spectrometry and ultraviolet spectrometry proved that the aldehyde existed in the metabolite. The structure of the metabolite in bile of rat was identified, that was 1-methyl-1-ethenyl-2-isopropenyl-4-isopropenyl aldehydohexamethylene.


CONCLUSIONS
The biotransformation of beta-elemene exists in vivo.
Li
Z
Z
Dalian Institute of Pharmaceutical and Medical Science, Dalian 116013, China.
Wang
K
K
Chen
Y R
YR
Wu
X Y
XY
Su
C Y
CY
D000818:Animals; D001646:Bile / Q000737:chemistry*; D007267:Injections; D008297:Male; D009822:Oils, Volatile / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012717:Sesquiterpenes
D009822:Oils, Volatile; D012717:Sesquiterpenes; C038905:elemene