Quartz glass pipette puller operating with a regulated oxy-hydrogen burner.
Quartz glass electrodes are superior to conventional glass electrodes for low-noise recording. They have better electrical characteristics and hydrophobic surfaces which resist creeping of salt solutions. We used oxy-hydrogen heating with program-controlled gas pressure to melt quartz glass capillaries. Usually, the relative wall thickness (the quotient of the outer and inner diameters do/di) of capillaries is, at best, maintained up to the electrode tip. If tips with thicker walls can be produced, coating and other surface treatments can be avoided. We found that programmed heating periods without pull allowed an fivefold increase of do/di in the tip region. Since do/di is inversely proportional to input capacity, the recording noise was minimized and became insignificant relative to amplifier and holder noise. A sample patch-clamp recording is shown.
Dudel
J
J
Institut für Physiologie, Technische Universität München, Munich, Germany. dudel@physiol.med.tu-muenchen.de
Hallermann
S
S
Heckmann
M
M
D000818:Animals; D004566:Electrodes; D005898:Glass; D006358:Hot Temperature; D006859:Hydrogen; D008564:Membrane Potentials; D051379:Mice; D009132:Muscles / Q000648:ultrastructure; D010100:Oxygen; D018408:Patch-Clamp Techniques / Q000295:instrumentation*; D011791:Quartz
D011791:Quartz; D006859:Hydrogen; D010100:Oxygen