Prostate cancer cell adhesion to quiescent endothelial cells is not mediated by beta-1 integrin subunit.
BACKGROUND
Previous studies have reported that tumor cells' adhesion to quiescent endothelial cell is mediated by beta-1 integrins. The aim of this study was to determine the role beta-1 integrins play in prostate cancer cell adhesion to human bone marrow endothelial cells (HBME) and human aortic endothelial cells (HAEC).


METHODS
A well described blocking antibody to beta-1 integrin subunit was used in adhesion assays to determine the role of beta-1 integrin subunit in the adhesion of PC-3 cells to both HBME cells and HAEC.


RESULTS
Antibody to the beta-1 integrin subunit failed to reduce PC-3 adhesion to HBME and HAEC, yet this same antibody significantly reduced adhesion of PC-3 cells to fibronectin coated wells.


CONCLUSIONS
The data suggest that metastasis of prostate cancer cells to bone may be mediated, in part, by preferential adhesion to HBME cells; but beta-1 integrins most likely are not involved in this interaction.
Cooper
C R
CR
University of Michigan Comprehensive Cancer Center, Department of Internal Medicine, Division of Hematology/Oncology, Ann Arbor, Michigan 48109, USA. cacooper@umich.edu
McLean
L
L
Mucci
N R
NR
Poncza
P
P
Pienta
K J
KJ
D000906:Antibodies / Q000494:pharmacology; D002448:Cell Adhesion / Q000502:physiology*; D004730:Endothelium, Vascular / Q000473:pathology*; D006801:Humans; D007074:Immunoglobulin G / Q000494:pharmacology; D019012:Integrin beta1 / Q000502:physiology*; D008297:Male; D011471:Prostatic Neoplasms / Q000473:pathology*
D000906:Antibodies; D007074:Immunoglobulin G; D019012:Integrin beta1