title: Identification of RAPD markers tightly linked to wheat powdery mildew resistance gene Pm6.l.
abstract: Pm6 transferred from Triticum timopheevii L. to common wheat, is an effective resistance gene to powdery mildew disease caused by Erysiphe graminis f. sp. tritici. The RAPD technique, employing a total of 700 decamer primers, was used to identify polymorphic markers between resistant (IGVI463) and susceptible (Prins) near-isogenic lines. Primer OPV20 produced a 2,000 base pair (bp) reproducible fragment only in the resistant near-isogenic line. The 2,000-bp DNA fragment was present in all other introgression lines containing Pm6. Using the F2 mapping population from a cross of IGVI-463 (PI170914/7*Prins) x Prins, Pm6 was shown to be closely linked to the marker OPV20-2000 at a genetic distance of 3.0 +/- 2.2 cM. The marker was successfully used in detecting the presence of Pm6 in different genetic backgrounds.
authors.affiliation: Key Open Lab of Agri. Ministry for Crop Cytogenetics, Nanjing Agri. Univ., Nanjing 210095, China.
authors.forename: X Y
authors.initials: XY
authors.lastname: Wang
authors.forename: Z J
authors.initials: ZJ
authors.lastname: Qi
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Ma
authors.forename: P D
authors.initials: PD
authors.lastname: Chen
authors.forename: D J
authors.initials: DJ
authors.lastname: Liu
mesh_terms: D001947:Breeding; D017343:Genes, Plant; D010935:Plant Diseases / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique; D014908:Triticum / Q000235:genetics*