title: [Kinetics and energy aspects of the effect of incubation medium dielectric permeability on the catalytic and transporting activity of Mg2+-ATP-dependent Ca2+ pump plasma membranes].
abstract: The results suggest that changes in polarity of the incubation medium markedly affect the activity of transport Ca2+, Mg(2+)-ATPase solubilized from smooth muscle cell plasma membranes and that the electrostatic interactions between the enzyme active center and specific ligands (MgADP-, in particular) significantly contribute to the energetics of ATP hydrolysis.
authors.affiliation: Palladine Institute of Biochemistry, NAS of Ukraine, Kyiv. kinet@biochem.kiev.ua
authors.forename: S O
authors.initials: SO
authors.lastname: Kosterin
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002384:Catalysis; D002462:Cell Membrane / Q000378:metabolism; D003470:Culture Media; D004553:Electric Conductivity; D004734:Energy Metabolism; D017136:Ion Transport; D007700:Kinetics
chemical_list: D003470:Culture Media; D000255:Adenosine Triphosphate; D002118:Calcium
vernacular_title: Kinetychni ta enerhetychni aspekty vplyvu dielektrychnoï proniknosti seredovyshcha inkubatsiï na katalitychnu ta transportnu aktyvnist' Mg2+,ATP-zalezhnoï kal'tsiievoï pompy plazmatychnoï membrany.