Enhanced macrophage functions and cytokine production of lymphocytes after ingestion of bon narine in female BALB/c mice.
The purpose of this investigation was to determine the effects of bon narine treatment on macrophage and lymphocyte functions in mice. Twelve week-old female inbred BALB/c mice were given bon narine p.o. at 30 mg/kg per day and sacrificed after three months. Glucose consumption of peritoneal macrophages in the bon narine treated group during incubation up to 72 h was significantly higher than that in the control group. Activities of acid phosphatase (APH), beta-glucuronidase (GLU) and lactate dehydrogenase (LDH) in the peritoneal macrophages in the bon narine treated group significantly increased compared to that in the control group. Macrophage production of nitric oxide stimulated by lipopolysaccharide (LPS) in the bon narine treated group was significantly increased. Interleukin-1beta (IL-1beta) production of peritoneal macrophages stimulated by LPS was significantly higher in the bon narine treated group. Stimulation indices in splenic lymphocytes by concanavalin A (Con A) in the bon narine treated group were significantly higher than that in the control group. Interleukin-2 (IL-2) and interferon-gamma (IFN-gamma) production stimulated by Con A were significantly increased in the bon narine treated mice. Interleukin-4 (IL-4) production of splenic lymphocytes stimulated by Con A was not different in the control group and the bon narine treated group. These findings might suggest that oral administration of bon narine effectively enhanced the macrophage function and lymphocyte responsiveness in mice.
Sugiura
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Department of Health and Physical Education, Gifu Pharmaceutical University, Japan. sugiura@gifu-pu.ac.jp
Sugiura
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Ueya
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Ueya
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Mirbod
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D000135:Acid Phosphatase / Q000378:metabolism; D000284:Administration, Oral; D000818:Animals; D002478:Cells, Cultured; D003208:Concanavalin A; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D005966:Glucuronidase / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000378:metabolism; D012016:Reference Values; D013154:Spleen / Q000276:immunology
D016207:Cytokines; D008070:Lipopolysaccharides; D003208:Concanavalin A; D009569:Nitric Oxide; D007770:L-Lactate Dehydrogenase; D000135:Acid Phosphatase; D005966:Glucuronidase