title: Schistosoma mansoni egg specific antibodies and circulating antigens: assessment of their validity in immunodiagnosis of schistosomiasis.
abstract: A total of 127 individuals of different age and sex; 92 from Kafr-Sendewa, Qualyobia Governorate, Egypt, in comparison to 23 cases with hydatidosis and fascioliasis as a parasitic control group, and 12 healthy control group from non-endemic area. All cases were screened by clinical examination, urine, stool, rectal snip, abdominal ultrasonographic examination and indirect haemagglutination test (IHAT). Accordingly, they were grouped into active intestinal schistosomiasis group, seropositive group by (IHAT), normal control group from the same endemic area, parasitic control group and normal control group. All cases were subjected to detection of IgG, IgM, IgG4, anti-soluble egg antigen (SEA) and anti-excretory-secretory egg antigen (ESEA) by ELISA tests; and circulating egg antigens by double-sandwich ELISA techniques. The results showed that IgG4 anti-SEA is the best diagnostic test, as it gave the best diagnostic efficacy (90%). Also, it is a good screening test which can be used in endemic area as it gave significant difference between the active intestinal schistosomiasis cases with each of the endemic control group (P < 0.001) and the seropositive cases (P < 0.05). Other valid diagnostic egg specific antibodies tests were IgM anti-SEA and IgG anti-SEA as their diagnostic efficacy were 80% and 76.6% respectively (P < 0.05). The diagnostic efficacy of circulating antigen detection (C.Ag) test was 70% (P < 0.05). In addition, it was the most specific test with 100% specificity. IG4-anti-ESEA gave the least cross-reaction with other parasites (17.3%). The mean optical density (OD) level of circulating antigen detection test was significantly higher in the organomegalic (hepatosplenomegaly) cases than the non-organomegalic cases (P < 0.05).
authors.affiliation: Department of Tropical Medicine, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt.
authors.forename: A A
authors.initials: AA
authors.lastname: Massoud
authors.forename: H M
authors.initials: HM
authors.lastname: Hussein
authors.forename: M A
authors.initials: MA
authors.lastname: Reda
authors.forename: H S
authors.initials: HS
authors.lastname: el-Wakil
authors.forename: K M
authors.initials: KM
authors.lastname: Maher
authors.forename: F S
authors.initials: FS
authors.lastname: Mahmoud
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis*; D000947:Antigens, Helminth / Q000032:analysis*; D002648:Child; D004443:Echinococcosis / Q000175:diagnosis; D004534:Egypt; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005211:Fascioliasis / Q000175:diagnosis; D005260:Female; D006386:Hemagglutination Tests; D006801:Humans; D008297:Male; D008875:Middle Aged; D012550:Schistosoma mansoni / Q000276:immunology*; D012552:Schistosomiasis / Q000175:diagnosis*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth