Effectiveness of propofol pretreatment on the extent of deranged cerebral mitochondrial oxidative enzyme system after incomplete forebrain ischemia/reperfusion in rats.
It has been suggested that propofol has the protective effect on cerebral ischemia-reperfusion injury. The aim of this study is to evaluate the effect of propofol pretreatment on incomplete forebrain ischemia-reperfusion injury in rats. Thirty Sprague-Dawley rats were anesthetized with isoflurane in oxygen and randomly allocated into propofol group (n=13) and saline group (n=17). In propofol group, propofol was pretreated in a step-down scheme before inducing forebrain ischemia by occlusion of both common carotid arteries and arterial hypotension. After ischemia (20 min) and reperfusion (30 min), rats were decapitated. Brain was sliced to obtain coronal slices of 4-12 mm from frontal pole, which were reacted with 2% 2,3,5-triphenyl-2H-tetrazolium chloride (TTC) for 10 min to differentiate the damaged tissues from normal tissues. Median (interquartile range) values of the average percent infarct area were 0.0 (8.6)% and 20.1 (41.2)% in propofol and saline groups, respectively. There was significant difference between the groups. In conclusion, propofol may have a protective effect on incomplete forebrain ischemia-reperfusion injury.
Lee
Y
Y
Department of Anesthesiology, Inje University, Sanggye Paik Hospital, Seoul, Korea. ylee@sanggyepaik.or.kr
Chung
C
C
Oh
Y S
YS
D000818:Animals; D002545:Brain Ischemia / Q000473:pathology / Q000517:prevention & control*; D002544:Cerebral Infarction / Q000473:pathology / Q000517:prevention & control; D004195:Disease Models, Animal; D016166:Free Radical Scavengers / Q000494:pharmacology*; D008928:Mitochondria / Q000201:enzymology*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010085:Oxidative Phosphorylation; D015742:Propofol / Q000494:pharmacology*; D016548:Prosencephalon / Q000187:drug effects* / Q000293:injuries / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000473:pathology / Q000517:prevention & control*; D013778:Tetrazolium Salts
D016166:Free Radical Scavengers; D018696:Neuroprotective Agents; D013778:Tetrazolium Salts; C009591:triphenyltetrazolium; D015742:Propofol