A random amplified polymorphic DNA polymerase chain reaction technique that differentiates between Neospora species.
Neospora caninum is a recently described coccidial parasite that was first isolated from a dog in 1988 and has subsequently been shown to infect a wide range of mammals. Neospora hughesi, a new species of this genus, has recently been isolated from the spinal cord of horses showing clinical signs of equine protozoal myeloencephalitis. The random amplified polymorphic DNA polymerase chain reaction technique is capable of differentiating between N. caninum and N. hughesi.
Spencer
J A
JA
Department of Pathobiology, College of Veterinary Medicine, Auburn University, Alabama 36849, USA.
Witherow
A K
AK
Blagburn
B L
BL
D000818:Animals; D003048:Coccidiosis / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*; D017931:DNA Primers / Q000737:chemistry; D016054:DNA, Protozoan / Q000032:analysis; D003937:Diagnosis, Differential; D004285:Dogs; D004679:Encephalomyelitis / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*; D006734:Horse Diseases / Q000175:diagnosis / Q000469:parasitology*; D006736:Horses; D018688:Neospora / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D019105:Random Amplified Polymorphic DNA Technique / Q000662:veterinary*; D013116:Spinal Cord / Q000469:parasitology
D017931:DNA Primers; D016054:DNA, Protozoan