title: Clinical correlation of cyclin D2 mRNA expression in human luteinized granulosa cells.
abstract: OBJECTIVE
  The aim was to evaluate whether the expression of cyclin D2 mRNA in human luteinized granulosa cells correlates with the clinical parameters of women undergoing in vitro fertilization-embryo transfer (IVF-ET) programs.
  
  
  METHODS
  Using reverse transcriptase-polymerase chain reaction (RT-PCR) techniques, the cyclin D2 mRNA in human luteinized granulosa cells of large follicles was measured. Correlation analysis between cyclin D2 mRNA expression and clinical parameters [age; day 3 follicle-stimulating hormone (FSH), luteinizing hormone (LH), and estradiol (E2); E2 and P4 at the day of human chorionic gonadotropin (hCG); total amount of gonadotropin and the number of days of gonadotropin administration; pregnancy outcome] were performed.
  
  
  RESULTS
  Cyclin D2 mRNA expression showed negative correlation with patient's age (R = -0.60, P < 0.005) and total amount of gonadotropins (R = -0.45, P < 0.03), and also showed positive correlation with serum E2 levels at the day of hCG administration (R = 0.55, P < 0.02). Other clinical parameters showed no statistical significance. Cyclin D2 mRNA expression of pregnant group was higher than that of nonpregnant group (P < 0.03).
  
  
  CONCLUSIONS
  Cyclin D2 mRNA expression in human luteinized granulosa cells positively correlates with clinical parameters of ovarian function and may be a novel marker for the health of human ovarian follicle.
authors.affiliation: Department of Obstetrics and Gynecology, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50 Ilwan-dong, Kangnam-ku, Seoul, Korea 135-710.
authors.forename: D
authors.initials: D
authors.lastname: Choi
authors.forename: E Y
authors.initials: EY
authors.lastname: Lee
authors.forename: S
authors.initials: S
authors.lastname: Yoon
authors.forename: S
authors.initials: S
authors.lastname: Hwang
authors.forename: B K
authors.initials: BK
authors.lastname: Yoon
authors.forename: J H
authors.initials: JH
authors.lastname: Lee
mesh_terms: D000328:Adult; D000367:Age Factors; D006063:Chorionic Gonadotropin / Q000097:blood / Q000627:therapeutic use; D056742:Cyclin D2; D016213:Cyclins / Q000235:genetics / Q000378:metabolism*; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D005640:Follicle Stimulating Hormone / Q000097:blood / Q000627:therapeutic use; D006107:Granulosa Cells / Q000378:metabolism*; D006801:Humans; D007986:Luteinizing Hormone / Q000097:blood / Q000378:metabolism*; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C080336:CCND2 protein, human; D006063:Chorionic Gonadotropin; D056742:Cyclin D2; D016213:Cyclins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone