title: [Signal transduction and biological function of IL-6 in a human myeloma cell line-XG-7].
abstract: OBJECTIVE
  To investigate the relationship between IL-6 signal transduction and its biological function on a human IL-6-dependent myeloma cell line-XG-7.
  
  
  METHODS
  Electrophoretic mobility shift (EMSA) and immunopeipitation were used respectively to detect the activation of transcription factors (STAT3 and NF-IL-6) and protein kinases (JAK1 and ERK) in the two IL-6 signal transduction pathways by IL-6 in XG-7 cells. Then the cells were transfected with the sense or anti-sense expression plasmids of the transcription factors, which could up- or down-regulate the activation of the signal transduction pathways specially, the changes of the biological function of IL-6 on XG-7 cells was shown by cell number counting and MTT.
  
  
  RESULTS
  IL-6 can promote the proliferation of XG-7 cells and activate one of the IL-6 signal transduction pathway-Ras/NF-IL-6; but Jak/STAT signal transduction pathway was not activated at the same conditions. When the activation of Ras/NF-IL-6 pathway was up- or down-regulated, the growth effect of IL-6 on XG-7 cells was strongthened or weakened.
  
  
  CONCLUSIONS
  The proliferation of XG-7 cells in the presence of IL-6 is mediated by the activation of Ras/NF-IL-6 signal transduction pathway.
authors.affiliation: Institute of Basic Medical Science, Academy of Military Medical Science, Beijing, 100850, China.
authors.forename: L
authors.initials: L
authors.lastname: Song
authors.forename: B
authors.initials: B
authors.lastname: Shen
mesh_terms: D002455:Cell Division / Q000187:drug effects; D006801:Humans; D015850:Interleukin-6 / Q000502:physiology*; D009101:Multiple Myeloma / Q000473:pathology / Q000503:physiopathology*; D015398:Signal Transduction / Q000502:physiology*; D014407:Tumor Cells, Cultured
chemical_list: D015850:Interleukin-6