title: Oxygen radical generation by emigrated, intra-abdominal, and circulating PMNLs during human secondary peritonitis.
abstract: The purpose of the study was to characterize oxygen radical generation by emigrated, intraabdominal and circulating polymorphonuclear leukocytes (ePMNLs and cPMNLs) during peritonitis, as well as to assess any differences between oxygen radical production in patients with low Mannheim peritonitis index (MPI < 26, group 1) or high Mannheim peritonitis index (MPI > or = 26, group 2). Lucigenin-enhanced chemiluminescence was used to determine spontaneous and stimulated (FMLP, PMA, and A23 187) oxygen radical generation by ePMNLs and cPMNLs. In group 1 spontaneous and stimulated oxygen radical generation by emigrated PMNLs was markedly enhanced compared to circulating PMNLs (e.g., spontaneous oxygen radical generation: 30.3 +/- 11.8 cpm/cPMNLs versus 107 +/- 46 cpm/ePMNLs, P < 0.05) . In group 2 oxygen radical generation by cPMNLs markedly increased within 48 h after diagnosis of peritonitis and surgery, contrary to radical generation by ePMNLs (e.g., A23 187-stimulated oxygen radical generation 993.7 +/- 350 cpm/cPMNLs versus 285.6 +/- 77 cpm/ePMNLs, P < 0.05. In conclusion, cPMNLs and ePMNLs exhibit marked polymorphism in their capacity to generate oxygen radicals in response to secondary peritonitis. Severe peritonitis (MPI - 26) was associated with a strong increase in oxygen radical generation by cPMNLs without a parallel activity being manifest by ePMNLs.
authors.affiliation: Department of General Surgery, Johann Wolfgang Goethe University, Frankfurt, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Holzer
authors.forename: A
authors.initials: A
authors.lastname: Richter
authors.forename: D
authors.initials: D
authors.lastname: Henrich
authors.forename: K
authors.initials: K
authors.lastname: Wilhelm
authors.forename: A
authors.initials: A
authors.lastname: Encke
mesh_terms: D000005:Abdomen / Q000601:surgery; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D002470:Cell Survival; D004731:Endotoxins / Q000097:blood; D005122:Exudates and Transudates / Q000378:metabolism; D005260:Female; D005609:Free Radicals; D006801:Humans; D016209:Interleukin-8 / Q000097:blood; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D010538:Peritonitis / Q000097:blood* / Q000401:mortality / Q000601:surgery; D011379:Prognosis; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D004731:Endotoxins; D005609:Free Radicals; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha; D010100:Oxygen