Properties of an adrenal medullary protein immunorelated to steroid acute regulatory (StAR) protein.
Immunohistochemistry using a StAR peptide antiserum had previously revealed strong staining in rat and bovine adrenal medulla, suggesting the presence of a protein immunogenically related to StAR. Western blots of bovine medulla tissue homogenates showed the principal adrenal medullary immuno-reactive species to have a higher molecular weight (50 kDa) compared to StAR protein (30 kDa). Subcellular fractionation localised the 50 kDa species principally to the medulla cytosol. StAR peptide antiserum binding to both the 30 kDa and 50 kDa species could be specifically competed by the peptide antigen. These data suggest that the adrenal medullary immuno-reactive species and StAR protein are distinct entities, which share some features in common.
Lo
Y C
YC
Department of Reproductive & Developmental Sciences, University of Edinburgh, Royal Infirmary, UK.
MacKenzie
S M
SM
Howie
A F
AF
Apps
D K
DK
Mason
J I
JI
Williams
B C
BC
Morley
S D
SD
D000313:Adrenal Medulla / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D003600:Cytosol / Q000378:metabolism; D007106:Immune Sera; D007150:Immunohistochemistry / Q000379:methods; D008970:Molecular Weight; D010750:Phosphoproteins / Q000737:chemistry / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D014018:Tissue Distribution; D000098682:Steroidogenic Acute Regulatory Protein
D007106:Immune Sera; D010750:Phosphoproteins; D011506:Proteins; D000098682:Steroidogenic Acute Regulatory Protein