Isolation and NMR characterisation of a (4-O-methyl-D-glucurono)-D-xylan from sugar beet pulp.
Stable aqueous suspensions of purified and homogenised sugar beet pulp (SBP) cellulose were subjected to various TFA treatments which induced a flocculation of the suspension and the release of a number of polysaccharides. Among these, a 4-O-methyl glucuronoxylan was isolated and characterized by 1H and 13C NMR spectroscopy. In this polysaccharide the molar proportions of D-Xyl and 4-O-Me-D-GlcA were found to be 7:1. The presence of a glucuronoxylan at the surface of the cellulose microfibrils is very likely involved in the stability of the suspensions. To our knowledge, the presence of a 4-O-methyl-glucuronoxylan in the sugar beet cells has not been described previously.
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Centre de Recherches sur les Macromolécules Végétales, CNRS, and Université Joseph Fourier, Grenoble, France.
Vignon
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D002482:Cellulose / Q000737:chemistry; D019663:Chenopodiaceae / Q000737:chemistry*; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D014269:Trifluoroacetic Acid; D014990:Xylans / Q000737:chemistry* / Q000302:isolation & purification
D014990:Xylans; D002482:Cellulose; C044967:4-O-methyl glucuronoxylan; D014269:Trifluoroacetic Acid