Acetobacterium tundrae sp nov, a new psychrophilic acetogenic bacterium from tundra soil.
A new psychrophilic, anaerobic, acetogenic bacterium from the tundra wetland soil of Polar Ural is described. The organism fermented H2/CO2, formate, methanol, and several sugars to acetate as the sole end-product. The temperature range for growth was 1-30 degrees C with an optimum at 20 degrees C. The bacterium showed no growth at 32 degrees C. Cells were gram-positive, oval-shaped, flagellated rods 0.7-1.l x 1.1-4.0 microm in size when grown at 1-20 degrees C. At 25-30 degrees C, the cell size increased up to 2-3 x 10-15 microm due to a defect in cell division. The DNA G+C content of the organism was 39.2 mol%. Based upon 16S rDNA analysis and DNA-DNA reassociation studies, the organism was classified in the genus Acetobacterium as a new species, for which the name Acetobacterium tundrae sp. nov. is proposed. The type strain is Z-4493 (=DSM 9173T).
Simankova
M V
MV
Institute of Microbiology of the Russian Academy of Sciences, Moscow. msimankova@mail.ru
Kotsyurbenko
O R
OR
Stackebrandt
E
E
Kostrikina
N A
NA
Lysenko
A M
AM
Osipov
G A
GA
Nozhevnikova
A N
AN
D000085:Acetates / Q000378:metabolism; D000693:Anaerobiosis; D001421:Bacteria, Anaerobic / Q000145:classification / Q000166:cytology / Q000302:isolation & purification / Q000502:physiology; D001482:Base Composition; D003081:Cold Climate; D003470:Culture Media; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D017753:Ecosystem; D006093:Gram-Positive Asporogenous Rods / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification* / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012426:Russia; D012988:Soil Microbiology; D013696:Temperature
D000085:Acetates; D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S