DNA electrochemical biosensors.
Disposable electrochemical DNA-based biosensors are reviewed; they have been used for the determination of low-molecular weight compounds with affinity for nucleic acids and for the detection of the hybridisation reaction. The first application is related to the molecular interaction between surface-linked DNA and the target pollutants or drugs, in order to develop a simple device for rapid screening of toxic or similar compounds. The determination of such compounds was measured by their effect on the oxidation signal of the guanine peak of calf thymus DNA immobilised on the electrode surface and investigated by chronopotentiometric analysis. The DNA biosensor is able to detect known intercalating compounds, such as daunomycin, polychlorinated biphenyls (PCBs), aflatoxin B1, and aromatic amines. Applicability to river and waste water samples is also demonstrated. Disposable electrochemical sensors for the detection of a specific sequence of DNA were realised by immobilising synthetic single-stranded oligonucleotides onto a graphite screen-printed electrode. The probes became hybridised with different concentrations of complementary sequences present in the sample. The hybrids formed on the electrode surface were evaluated by chronopotentiometric analysis using daunomycin as indicator of the hybridisation reaction. The hybridisation was also performed using real samples. Application to apolipoprotein E (ApoE) is described, in this case samples have to be amplified by PCR and then analysed by DNA biosensor. The extension of such procedures to samples of environmental interest or to contamination of food is discussed.
Mascini
M
M
Dipartimento di Sanità Pubblica, Epidemiologia e Chimica Analitica Ambientale, Università di Firenze, Via G. Capponi, 9, 50121, Firenze, Italy. mascini@unifi.it
Palchetti
I
I
Marrazza
G
G
D001057:Apolipoproteins E / Q000097:blood / Q000235:genetics; D015374:Biosensing Techniques; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D005618:Fresh Water / Q000032:analysis; D006801:Humans; D007220:Industrial Waste / Q000032:analysis; D009153:Mutagens / Q000032:analysis; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D001057:Apolipoproteins E; D007220:Industrial Waste; D009153:Mutagens; D009841:Oligonucleotides; D004247:DNA