title: Dermal fibroblasts do not enhance the graft take rate of autologous, cultured keratinocyte suspension on full-thickness wounds in rats.
abstract: Dermal fibroblasts are known to play an important role in wound healing. In this study, cultured autologous keratinocyte suspension was applied with fibrin glue to the full-thickness wounds in rats (N = 20). Histological analysis on day 14 showed regenerated epithelium in 10 wounds (50%). Keratinocytes were also premixed with allogeneic dermal fibroblasts in a ratio of 3:1 and 5:1 before application to other full-thickness wounds (N = 20) with fibrin glue. Regeneration of epithelium was observed in 10 (50%) and 9 (45%) wounds respectively. Acute inflammatory reaction and mild to moderate proliferation of fibroblasts in the subepithelial layer of the allogeneic fibroblasts were noted. The addition of dermal fibroblasts to keratinocytes/fibrin glue does not enhance the take rate of the cultured keratinocyte suspension.
authors.affiliation: Hong Kong Skin Bank, Department of Surgery, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, NT.
authors.forename: P K
authors.initials: PK
authors.lastname: Lam
authors.forename: E S
authors.initials: ES
authors.lastname: Chan
authors.forename: C T
authors.initials: CT
authors.lastname: Liew
authors.forename: R S
authors.initials: RS
authors.lastname: Yen
authors.forename: H C
authors.initials: HC
authors.lastname: Lau
authors.forename: W W
authors.initials: WW
authors.lastname: King
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D062109:Dermatologic Surgical Procedures; D015718:Fibrin Tissue Adhesive / Q000627:therapeutic use; D015603:Keratinocytes / Q000502:physiology* / Q000637:transplantation*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000166:cytology; D014182:Transplantation, Autologous; D014945:Wound Healing / Q000502:physiology
chemical_list: D015718:Fibrin Tissue Adhesive