Protective effects of green tea catechins against asbestos-induced cell injury.
Green tea extract was found to provide a strong protective effect against asbestos-induced injury of peritoneal macrophages and red blood cells in vitro. The main polyphenolic constituents of green tea extract, (-)-epicatechin gallate (ECG) and (-)-epigallocatechin gallate (EGCG), were also efficient in preventing injury of cells following exposure to asbestos fibers. The protective efficacies of EGCG and ECG expressed as IC50 values were, respectively, 10 microM and 12 microM if peritoneal macrophages were injured by chrysotile and 4 microM and 5 microM in the case of crocidolite-induced cell injury. Antiradical and chelating properties of ECG and EGCG were evaluated and it was concluded that the protective effect of catechins against asbestos-induced injury may be related to both scavenger properties towards to superoxide anion and the ability to chelate iron ions.
Kostyuk
V A
VA
Potapovich
A I
AI
Vladykovskaya
E N
EN
Hiramatsu
M
M
D000818:Animals; D001194:Asbestos / Q000633:toxicity*; D002392:Catechin / Q000494:pharmacology*; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects*; D066298:In Vitro Techniques; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000187:drug effects*; D051381:Rats; D013662:Tea / Q000737:chemistry*
D013662:Tea; D001194:Asbestos; D002392:Catechin