title: [Comparison of various PCR methods for the detection of Chlamydia trachomatis, Ureaplasma urealyticum, and Mycoplasma hominis in women with impaired reproductive function].
abstract: The comparative evaluation of the PCR test "Polimik" (Research and Production Firm "Litekh", Moscow) and the PCR test of the Novosibirsk Institute of Bioorganic Chemistry (NIBC) was carried out. The results obtained with the use of the PCR test "Polimik" and the PCR test of the NIBC of the detection of C. trachomatis and M. hominis coincided in 97.8% and 97.4% of cases. For U. urealyticum, the coincidence of the results of both PCR tests was 81.2%. Among women who visited gynecologists for reproductive function disturbances, the use of the PCR tests made it possible to detect C. trachomatis in 19 (5.5%) out of 343 cases, U. urealyticum in 96 (39.0%) out of 246 cases and M. hominis in 25 (16.9%) out of 148 cases. The results of the investigation revealed that the occurrence of C. trachomatis infection in Novosibirsk was comparable with that in other regions of the world among the low-risk groups of the population. The detection frequency of M. hominis and U. urealyticum with the use of the PCR tests showed that the occurrence of infections caused by these causative agents coincided with the data obtained in other countries.
authors.affiliation: Institute of Bioorganic Chemistry, Novosibirsk, Russia.
authors.forename: E G
authors.initials: EG
authors.lastname: Rempel'
authors.forename: V A
authors.initials: VA
authors.lastname: Shamanin
mesh_terms: D002692:Chlamydia trachomatis / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis*; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000150:complications*; D019535:Mycoplasma hominis / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D015231:Sexually Transmitted Diseases, Bacterial / Q000150:complications / Q000382:microbiology*; D016990:Ureaplasma urealyticum / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial
vernacular_title: Sravnenie raznykh PTsR-testov dlia vyiavleniia Chlamydia trachomatis, Ureaplasma urealyticum i Mycoplasma hominis.