title: Covalent grafting of polythymidylic acid onto amine-containing polystyrene latex particles.
abstract: The covalent grafting of a 35-base poly(thymidylic) acid (dT35) onto aminated polystyrene latex has been studied. The oligonucleotide was activated by 1,4-phenylene diisothiocyanate (PDC) prior to grafting reaction. The influence of various experimental conditions related either to the medium (pH, ionic strength) or to the latex (nature and surface charge density, presence of adsorbed nonionic surfactant) was studied. Discrimination between covalent grafting and adsorption was achieved by desorbing the non-covalently grafted dT35 induced by consecutive washings with a suitable buffer solution. The amount of covalently grafted oligonucleotide directly correlated to the concentration and accessible functional groups at the particle surface.
authors.affiliation: Unité Mixte CNRS-bioMérieux ENS, Lyon, France.
authors.forename: F
authors.initials: F
authors.lastname: Ganachaud
authors.forename: A
authors.initials: A
authors.lastname: Elaissari
authors.forename: C
authors.initials: C
authors.lastname: Pichot
mesh_terms: D000327:Adsorption; D000588:Amines / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D019075:Electrophoresis, Capillary; D006863:Hydrogen-Ion Concentration; D007477:Ions; D007700:Kinetics; D007840:Latex / Q000737:chemistry*; D008956:Models, Chemical; D011071:Poly T / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry; D013997:Time Factors
chemical_list: D000588:Amines; D004277:DNA, Single-Stranded; D007477:Ions; D007840:Latex; D011137:Polystyrenes; D013501:Surface-Active Agents; D011071:Poly T