title: [Construction and nucleotide sequence assay of human anti-HBs variable region single-chain antibody gene].
abstract: The VH and Vk gene which were acquired using phage display technology have been combined by a linker encoding a flexible peptide (Gly4 Ser)3 to form a single-chain. Its nucleotide sequence was assayed after cloning the scFv gene into middle plasmid. The successful construction of single-chain gene lays the foundations for the subsequent expression and production of double functional antibody.
authors.affiliation: Central Research Institution of Infectious Disease, 458th Hospital of PLA, Guangzhou 510602.
authors.forename: X
authors.initials: X
authors.lastname: Ren
authors.forename: H
authors.initials: H
authors.lastname: Gao
authors.forename: K
authors.initials: K
authors.lastname: Su
authors.forename: W
authors.initials: W
authors.lastname: Chen
authors.forename: A
authors.initials: A
authors.lastname: Yang
authors.forename: Z
authors.initials: Z
authors.lastname: Yu
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D006510:Hepatitis B Antibodies / Q000235:genetics*; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
chemical_list: D006510:Hepatitis B Antibodies; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D007145:Immunoglobulin kappa-Chains