title: A VSV-G pseudotyped HIV vector mediates efficient transduction of human pulmonary artery smooth muscle cells.
abstract: Attempts were made to infect human vascular smooth muscle cells derived from the pulmonary artery (hPASMC) with two different human immunodeficiency virus (HIV) vector systems. ADA/Luc or HXB2/Luc were generated by cotransfection of luciferase reporter gene vector, pNL4-3-Luc-E- R-, and one of two envelope expressing vectors, pSMADA (R5) or pSMHXB2 (X4). The VSV-G/Luc or VSV-G/GFP were produced by a three-plasmid expression system which consisted of vesicular stomatitis virus G protein (VSV-G) expressing vector, packaging plasmid, and one of two reporter genes (pHR'-CMV-Luc or pHR'-CMV-GFP). We used hPASMC, U87.CD4.CCR5 and U87.CD4.CXCR4 for infection. Neither ADA/Luc nor HXB2/Luc could infect hPASMC, though they could infect U87.CD4 with corresponding coreceptors. On the other hand, the transduction of both VSV-G/Luc and VSV-G/GFP to hPASMC was remarkable. At day 3, the relative proportion of positive cells of hPASMC infected with VSV-G/GFP was 15%. The above finding indicates a direct role of HIV-1 infection in pulmonary hypertension 'a rare complication of HIV-1 infection' and HIV-based vectors could introduce foreign genes into hPASMC for gene therapy of pulmonary hypertension.
authors.affiliation: Division of Cardiovascular Medicine, Internal Medicine, Graduate School of Medicine, Tohoku University, Sendai, Miyagi, Japan.
authors.forename: S L
authors.initials: SL
authors.lastname: Li
authors.forename: X Y
authors.initials: XY
authors.lastname: Zhang
authors.forename: H
authors.initials: H
authors.lastname: Ling
authors.forename: J
authors.initials: J
authors.lastname: Ikeda
authors.forename: K
authors.initials: K
authors.lastname: Shirato
authors.forename: T
authors.initials: T
authors.lastname: Hattori
mesh_terms: D002460:Cell Line; D002461:Cell Line, Transformed; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000235:genetics / Q000502:physiology*; D049452:Green Fluorescent Proteins; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008156:Luciferases / Q000235:genetics; D008164:Luminescent Proteins / Q000235:genetics; D008562:Membrane Glycoproteins; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D011651:Pulmonary Artery / Q000166:cytology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000502:physiology*
chemical_list: C028391:G protein, vesicular stomatitis virus; D008164:Luminescent Proteins; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins; D049452:Green Fluorescent Proteins; D008156:Luciferases