Enzyme-linked immunosorbent assays of canine apolipoproteins B-100 and A-I.
An enzyme-linked immunosorbent assay (ELISA) for canine blood apolipoprotein (apo) B-100 and A-I was developed. The working range for the assay was 1.8 to 28.7 ng/well for apoB-100 and it was 50 to 410 ng/well for apoA-I. The intra- and inter-assay coefficients of variation for the assay for apoB-100 were 5.4 and 6.9%, respectively, and for apoA-I they were 5.8 and 10.6%, respectively. The average concentrations of apoB-100 and A-I in 25 beagles (males, aged 3-4 years) were 0.084 +/- 0.028 (mean +/- SD) mg/ ml and 6.29 +/- 1.55 mg/ml, respectively. The ratios of canine (C) apoB-100 to CapoA-I were 1.41 +/- 0.58%. The respective concentrations in one case of hyperlipidemia with systemic atherosclerosis were 0.454 mg/ml and 11.28 mg/ml (a ratio of 4.03%). These values were larger than those of the controls. These results suggest that the measurements of CapoB-100 and A-I concentrations by this newly developed ELISA are helpful for diagnosis of lipidosis.
Miyoshi
K
K
Department of Veterinary Internal Medicine II, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan.
Uchida
E
E
Niiyama
M
M
D000818:Animals; D016632:Apolipoprotein A-I / Q000097:blood*; D053299:Apolipoprotein B-100; D001055:Apolipoproteins B / Q000097:blood*; D001161:Arteriosclerosis / Q000175:diagnosis / Q000662:veterinary*; D015153:Blotting, Western / Q000662:veterinary; D002784:Cholesterol / Q000097:blood; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D006949:Hyperlipidemias / Q000662:veterinary; D008297:Male; D014280:Triglycerides / Q000097:blood
D016632:Apolipoprotein A-I; D053299:Apolipoprotein B-100; D001055:Apolipoproteins B; D014280:Triglycerides; D002784:Cholesterol