LIF is more potent than BDNF in promoting neurite outgrowth of mammalian auditory neurons in vitro.
Neurotrophic factors are known to play a crucial role in the elongation and guidance of auditory nerve fibres to their targets within the organ of Corti. Maintenance of these neural connections following deafness would clearly influence the efficacy of therapies for hearing recovery. The growth factors leukaemia inhibitory factor (LIF), brain-derived neurotrophic factor (BDNF) and transforming growth factor-beta 5 (TGF-beta5) were tested for their efficacy in promoting neurite outgrowth from dissociated cultures of early postnatal rat auditory neurons. Our results indicate that while BDNF enhances neurite outgrowth in a strong fashion, LIF is more potent; moreover, the combined administration of both factors has even greater neuritogenic capacities. TGF-beta5, although neurotrophic, has no neuritogenic activity on cultured auditory neurons. LIF and BDNF may therefore be potential candidates when developing pharmacological therapies for hearing recovery.
Gillespie
L N
LN
Department of Otolaryngology, The University of Melbourne, Royal Victorian Eye and Ear Hospital, East Melbourne, Australia.
Clark
G M
GM
Bartlett
P F
PF
Marzella
P L
PL
D000818:Animals; D001306:Auditory Pathways / Q000166:cytology*; D001369:Axons / Q000187:drug effects / Q000502:physiology; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology*; D002478:Cells, Cultured; D066298:In Vitro Techniques; D008222:Lymphokines / Q000494:pharmacology*; D008322:Mammals; D016501:Neurites / Q000187:drug effects* / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley
D019208:Brain-Derived Neurotrophic Factor; D008222:Lymphokines; C010598:leukocyte inhibitory factor