Relationship between antigen release and antibody response of infectious coryza water-in-oil-in-water emulsion vaccines.
The relationship between the antigen release from formulations in vitro and the antibody response after administration of water-in-oil-in-water (W/O/W) emulsion vaccines containing Haemophilus paragallinarum (Hpg) was studied in chickens. Increases of sorbitan sesquioleate volume in the formulation led to slower antigen release and tended to induce higher hemagglutination-inhibition (HI) antibody titers. In addition, the vaccines prepared with internal aqueous phase:oil phase:external aqueous phase (A:O:A) ratios of 3:4:3 and 3:3:4 also showed slower release of antigen and higher HI antibody titer compared with those of an A:O:A ratio of 3:2:5. Vaccines prepared with polyoxyethylene (POE)(10) hydrogenated castor oil or POE(40) hydrogenated castor oil instead of sorbitan sesquioleate showed higher release and lower antibody HI titers. As a result, HI antibody titers at 6 wk after vaccination were inversely related to antigen release, as determined by the release test. The correlation coefficient was 0.942. In infectious coryza W/O/W emulsion vaccines, the slow release of antigen from the formulation induced and maintained high HI antibody titers of Hpg.
Fukanoki
S
S
Aburahi Laboratories Shionogi and Co., Ltd., Shiga, Japan.
Matsumoto
K
K
Mori
H
H
Takeda
R
R
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology* / Q000378:metabolism; D000942:Antigens, Bacterial / Q000276:immunology* / Q000378:metabolism; D001428:Bacterial Vaccines / Q000276:immunology*; D002645:Chickens; D004655:Emulsions; D006190:Haemophilus / Q000276:immunology*; D006601:Hexoses / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000494:pharmacology; D015164:Vaccines, Inactivated / Q000276:immunology; D014867:Water
D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D004655:Emulsions; D006601:Hexoses; D015164:Vaccines, Inactivated; D014867:Water; C058854:sorbitan sesquioleate; D011092:Polyethylene Glycols