title: The role of lipid peroxidation in aluminium toxicity in soybean cell suspension cultures.
abstract: The primary reactions leading to Al toxicity in plant cells have not yet been elucidated. We used soybean (Glycine max [L.] Merr.) cell suspension cultures to address the question whether lipid peroxidation plays an important role in Al toxicity. Upon transfer to an Al-containing culture medium with a calculated Al3+ activity of 15 microM soybean cells showed a distinct and longtime increase in lipid peroxidation within 4 h. At the same time a drastic loss of cell viability was observed. Butylated hydroxyanisole (BHA) and N,N'-diphenyl-p-phenylenediamine (DPPD), two lipophilic antioxidants, were able to almost completely suppress lipid peroxidation in Al-treated cells at a concentration of 20 microM. This effect was dose-dependent for DPPD and was observed at minimum concentrations of 1-2 microM. When lipid peroxidation was suppressed by DPPD or BHA cell viability remained high even in the presence of toxic Al concentrations. These results suggest that Al-induced enhancement of lipid peroxidation is a decisive factor for Al toxicity in suspension cultured soybean cells.
authors.affiliation: Institut für Biochemie und Biotechnologie der Pflanzen, Westfälische Wilhelms-Universität Münster, Germany.
authors.forename: I
authors.initials: I
authors.lastname: Rath
authors.forename: W
authors.initials: W
authors.lastname: Barz
mesh_terms: D000535:Aluminum / Q000633:toxicity*; D000975:Antioxidants / Q000494:pharmacology*; D002083:Butylated Hydroxyanisole / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D007700:Kinetics; D015227:Lipid Peroxidation / Q000187:drug effects / Q000502:physiology*; D010655:Phenylenediamines / Q000494:pharmacology; D013025:Glycine max / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D013997:Time Factors
chemical_list: D000975:Antioxidants; D010655:Phenylenediamines; D002083:Butylated Hydroxyanisole; D000535:Aluminum; C004845:N,N'-diphenyl-4-phenylenediamine; D004121:Dimethyl Sulfoxide