title: Translocation of Bacillus anthracis lethal and oedema factors across endosome membranes.
abstract: The two exotoxins of Bacillus anthracis, the causative agent of anthrax, are the oedema toxin (PA-EF) and the lethal toxin (PA-LF). They exert their catalytic activities within the cytosol. The internalization process requires receptor-mediated endocytosis and passage through acidic vesicles. We investigated the translocation of EF and LF enzymatic moieties across the target cell membrane. By selective permeabilization of the plasma membrane with Clostridium perfringens delta-toxin, we observed free full-size lethal factor (LF) within the cytosol, resulting from specific translocation from early endosomes. In contrast, oedema factor (EF) remained associated with the membranes of vesicles.
authors.affiliation: Unité Toxines et Pathogénie Bactériennes, CNRS URA1858, Institut Pasteur, Paris, France. rontani@pasteur.fr
authors.forename: C
authors.initials: C
authors.lastname: Guidi-Rontani
authors.forename: M
authors.initials: M
authors.lastname: Weber-Levy
authors.forename: M
authors.initials: M
authors.lastname: Mock
authors.forename: V
authors.initials: V
authors.lastname: Cabiaux
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial; D001408:Bacillus anthracis / Q000378:metabolism*; D001427:Bacterial Toxins / Q000378:metabolism*; D002352:Carrier Proteins / Q000502:physiology*; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology*; D003600:Cytosol / Q000378:metabolism; D011992:Endosomes / Q000502:physiology*; D008264:Macrophages / Q000382:microbiology; D051379:Mice
chemical_list: D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D002352:Carrier Proteins; C030325:anthrax toxin