title: In vitro evaluation of the biofunctionality of osteoblasts cultured on DegraPol-foam.
abstract: The biofunctionality of osteoblasts cultured on DegraPol-foam, a biodegradable, elastic, and highly porous polyesterurethane-foam, was determined here to examine the possible use of this structure as bone repair material. Osteoblasts from rat tibia and from the cell line (MC3T3-E1) exhibited relatively high attachment and low doubling time that result in a confluent cell multilayer on the surface of the foam. They produced high concentrations of collagen type I and osteocalcin, and expressed increasing alkaline phosphatase activity. Exposure to 1,25-dihydroxy vitamin D (Vit. D) increased dose- and time-dependent alkaline phosphatase activity and osteocalcin concentration, and decreased the level of collagen type I and cell density. Maximal effects of Vit. D on alkaline phosphatase activity (2.2 fold), osteocalcin (1.5 fold), collagen type I (50% reduction), and on cell density (35% reduction) were found at 100 ng Vit. D ml(-1). Osteoblasts cultured on DegraPol-foam in the presence of Vit. D exhibited more spreading and less spindle-like morphology than cells cultured in the absence of Vit. D. Cell ingrowth into the pores of the foam was not affected by Vit. D treatment. Taken collectively, the osteoblasts, capability of responding to Vit. D confirms the osteoblast compatibility of DegraPol-foam and the possible use of this scaffold in the bone healing process.
authors.affiliation: Research Division, Department of Surgery, University Hospital, Zurich, Switzerland.
authors.forename: B
authors.initials: B
authors.lastname: Saad
authors.forename: M
authors.initials: M
authors.lastname: Casotti
authors.forename: T
authors.initials: T
authors.lastname: Huber
authors.forename: P
authors.initials: P
authors.lastname: Schmutz
authors.forename: M
authors.initials: M
authors.lastname: Welti
authors.forename: G K
authors.initials: GK
authors.lastname: Uhlschmid
authors.forename: P
authors.initials: P
authors.lastname: Neuenschwander
authors.forename: U W
authors.initials: UW
authors.lastname: Suter
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D018786:Bone Substitutes / Q000737:chemistry / Q000494:pharmacology*; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003094:Collagen / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D019084:Fluorescent Antibody Technique, Indirect; D008297:Male; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology / Q000648:ultrastructure; D015675:Osteocalcin / Q000096:biosynthesis; D010641:Phenotype; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology*; D011140:Polyurethanes / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014807:Vitamin D / Q000494:pharmacology
chemical_list: D018786:Bone Substitutes; C104154:DegraPol; D011091:Polyesters; D011140:Polyurethanes; D015675:Osteocalcin; D014807:Vitamin D; D003094:Collagen; D000469:Alkaline Phosphatase