Dietary beta- and gamma-cyclodextrins stimulation of hepatic metallothionein gene expression in rats.
This study investigated whether hepatic metallothionein gene expression is affected by dietary cyclodextrins. Young male Wistar rats were fed a basal diet or cyclodextrin-supplemented (50 g of cyclodextrin per kg diet) diets for 7 d. Copper content in the liver did not show any significant changes among rats fed the basal, beta- and gamma-cyclodextrin diets. There were no differences in liver or serum zinc among groups. Copper content in serum was markedly decreased in rats fed the gamma-cyclodextrin-supplemented diet. Liver metallothionein mRNA levels were significantly elevated in both beta- and gamma-cyclodextrins-fed rats, but not in alpha-cyclodextrin-fed rats. Thus, the increase in hepatic metallothionein mRNA levels might be due to this mechanism except for the contents of copper and zinc in the liver.
Kaewprasert
S
S
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan.
Okada
M
M
Aoyama
Y
Y
D000818:Animals; D015152:Blotting, Northern; D003300:Copper / Q000378:metabolism; D003505:Cyclodextrins / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D008297:Male; D008668:Metallothionein / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015032:Zinc / Q000378:metabolism; D047392:beta-Cyclodextrins; D047408:gamma-Cyclodextrins
D003505:Cyclodextrins; D012333:RNA, Messenger; D047392:beta-Cyclodextrins; D047408:gamma-Cyclodextrins; D003300:Copper; D008668:Metallothionein; D015032:Zinc; C031215:betadex; C023792:gamma-cyclodextrin