title: Organization of the rat PACAP gene.
abstract: The rat PACAP gene has been isolated, cloned, and sequenced. The gene organization is very similar to that of the mouse and human PACAP genes. An analysis of the 5'-UTR in proPACAP mRNAs indicates that multiple transcripts are generated by using different splice sites within exons 1A, 1B, and 2 to form the 5'-UTR. Analysis of PACAP promoter activity in PC12 cells suggests that forskolin responsiveness of the gene is dependent on elements located between -77 and -413 bp from the transcription start site.
authors.affiliation: Department of Anatomy and Neurobiology, University of Vermont College of Medicine, Burlington, Vermont 05405, USA. slw@salus.uvm.edu
authors.forename: S L
authors.initials: SL
authors.lastname: White
authors.forename: V
authors.initials: V
authors.lastname: May
authors.forename: K M
authors.initials: KM
authors.lastname: Braas
mesh_terms: D020121:5' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons; D006801:Humans; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics*; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D011498:Protein Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D012689:Sequence Homology, Nucleic Acid
chemical_list: D020121:5' Untranslated Regions; C495401:ADCYAP1 protein, human; C495402:Adcyap1 protein, mouse; C495403:Adcyap1 protein, rat; D018076:DNA, Complementary; D009479:Neuropeptides; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D011498:Protein Precursors; D012333:RNA, Messenger