Baboon cytochrome P450c11 is encoded by more than one gene.
Two P450c11 genes, sharing 99% homology, were cloned from Cape baboon adrenal tissue using RT-PCR. The cDNAs showed 96% and 94.6% sequence identity to human P450c11 and aldosterone synthase, respectively. One of the gene sequences contained a termination codon in exon one. The cloned cDNAs were expressed in COS 1 cells and the metabolism of deoxycorticosterone to corticosterone investigated. The expressed enzyme exhibited 1 beta hydroxylase activity, but no aldosterone synthase activity.
Swart
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AC
Department of Biochemistry, University of Stellenbosch, South Africa.
Brown
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N
Kolar
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Swart
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P
D000311:Adrenal Glands / Q000737:chemistry; D000818:Animals; D019556:COS Cells; D003001:Cloning, Molecular; D003062:Codon / Q000235:genetics; D003345:Corticosterone / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D003900:Desoxycorticosterone / Q000378:metabolism; D005091:Exons / Q000235:genetics; D015870:Gene Expression; D006899:Mixed Function Oxygenases / Q000737:chemistry / Q000235:genetics*; D010215:Papio / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013252:Steroid 11-beta-Hydroxylase / Q000378:metabolism
D003062:Codon; D018076:DNA, Complementary; D003900:Desoxycorticosterone; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; C421925:cytochrome P450 C11; D013252:Steroid 11-beta-Hydroxylase; D003345:Corticosterone