title: Indirect fluorescence detection of phenolic compounds by capillary electrophoresis on a glass device.
abstract: A micromachined capillary electrophoresis system has been fabricated on a glass device for the separation and indirect fluorescence detection of phenols. Using this device two phenols viz., 2,4-dichlorophenol and pentachlorophenol, were separated within 12 s compared to under 19 min on a conventional capillary electrophoresis system using direct ultraviolet detection. The precision of the glass device ranged from 12.7%-16.7% compared to 0.42%-4.9% for the conventional system. Both systems showed good linearity in the concentration range of 0.8-6.38 mM for the glass device and 5-130 microM for the conventional system. The relationship between temperature and high voltage with baseline drift was also investigated. These results provide a foundation for the development of a miniaturised chemical analysis system for the on-line analysis of phenols in water.
authors.affiliation: Zeneca/Smithkline Beecham Centre for Analytical Sciences, Imperial College of Science, Technology & Medicine, London, United Kingdom.
authors.forename: M
authors.initials: M
authors.lastname: Arundell
authors.forename: P D
authors.initials: PD
authors.lastname: Whalley
authors.forename: A
authors.initials: A
authors.lastname: Manz