Nucleotides LXIV[1]: synthesis hydridization and enzymatic degradation studies of 2'-O-methyloligoribonucleotides and 2'-O-methyl/deoxy gapmers.
2'-O-Methyloligoribonucleotides, deoxyoligonucleotides and 2'-O-methyl/deoxy gapmers were synthesized using solid phase phosphoramidite chemistry employing the 2-(4-nitrophenyl)ethyl (npe) protection strategy. Melting temperatures of the synthesized oligonucleotides as well as their stability against degradation by several different nucleases were determined. 2'-O-Methyloligoribonucleotides showed the highest melting temperatures (Tm's) whereas 2'-O-methyl/deoxy gapmers revealed either slightly higher or surprizingly no thermal stabilities compared with their deoxy analogs when using self-complementary sequences. Gapmers with four 2'-O-methyl nucleotides on both ends showed about the same stability as all 2'-O-methyloligoribonucleotides against micrococal nuclease, nuclease S1, and snake venom phosphodiesterase.
Cramer
H
H
Fakultät für Chemie, Universität Konstanz, Germany.
Pfleiderer
W
W
D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D006868:Hydrolysis; D008836:Micrococcal Nuclease / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism*; D013696:Temperature
D009841:Oligonucleotides; D015719:Single-Strand Specific DNA and RNA Endonucleases; D008836:Micrococcal Nuclease; D010727:Phosphoric Diester Hydrolases