A comparison between human, crustacean and bivalve isolates of Microsporidia.
Identification of Microsporidia detected in diarrhoeic stool of human and from press preparation of crabs, and bivalves muscles was done by staining with Giemsa, modified trichrome and H & E stains respectively. Positive samples were isolated & used for infection of 3 groups of mice viz group A human isolate, group B crab & group C bivalve isolates. Parasitological and histopathological studies using light and transmission electron microscopy (TEM) were done for samples from each group. The results clarified that human, crab & bivalve Microsporidia isolates showed common features. They were found in the supranuclear region of enterocytes. The stages observed were meronts, sporonts and free spores. Each spore was 1-2 um in length containing a single nucleus, a vacuole and a polar filament. Further genetical and biochemical studies will be undertaken to confirm the similarity or differences between these isolates.
Awadalla
H N
HN
Department Parasitology, Faculty of Medicine, Alexandria University, Alexandria, Egypt.
Khalifa
A M
AM
Sadaka
H A
HA
Helal
S M
SM
D000818:Animals; D003445:Crustacea / Q000469:parasitology*; D003967:Diarrhea / Q000469:parasitology; D005243:Feces / Q000469:parasitology; D006801:Humans; D051379:Mice; D008854:Microscopy, Electron; D016814:Microsporidia / Q000302:isolation & purification* / Q000648:ultrastructure