Attachment of human gingival fibroblasts to periodontally involved root surface following scaling and/or etching procedures: a scanning electron microscopy study.
This study evaluated, in vitro, fibroblast attachment to periodontally involved root surfaces which were either root planed or acid/chelated by different agents. Specimens were divided into 3 groups of 12 specimens each. The root surfaces were root planed with a Gracey 7/8 curette, an EMS or an Amdent piezo-electric scaler and treated with saline, citric acid, tetracycline hydrochloride or EDTA to produce different surface textures. They were then cultured with fibroblasts for 72 h and examined by scanning electron microscopy. There was a significantly greater number of fibroblasts attached to specimens treated with citric acid, tetracycline and EDTA than to those root planed only. Furthermore, fibroblasts were more likely to attach to rough-surfaced than to smooth-surfaced specimens.
Babay
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Division of Periodontics, Department of Preventive Dental Sciences, King Saud University College of Dentistry, P.O. Box 60169, Riyadh 11545, Kingdom of Saudi Arabia. nbabay@ksu.edu.sa
D000134:Acid Etching, Dental; D002448:Cell Adhesion; D002478:Cells, Cultured; D002614:Chelating Agents / Q000008:administration & dosage; D019343:Citric Acid / Q000008:administration & dosage; D003094:Collagen / Q000648:ultrastructure; D003475:Curettage / Q000295:instrumentation; D012534:Dental Scaling / Q000295:instrumentation; D003804:Dentin / Q000473:pathology; D004492:Edetic Acid / Q000008:administration & dosage; D005347:Fibroblasts / Q000473:pathology*; D005881:Gingiva / Q000473:pathology*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010510:Periodontal Diseases / Q000473:pathology / Q000628:therapy*; D016745:Root Planing / Q000295:instrumentation; D012965:Sodium Chloride; D013752:Tetracycline / Q000008:administration & dosage; D014092:Tooth Root / Q000187:drug effects / Q000473:pathology*
D002614:Chelating Agents; D019343:Citric Acid; D012965:Sodium Chloride; D003094:Collagen; D004492:Edetic Acid; D013752:Tetracycline