title: B-type natriuretic peptide (BNP)--validation of an immediate response assay.
abstract: B-type natriuretic peptide, a proteohormone secreted by the left ventricle in response to wall-tension, is a promising laboratory parameter for the detection and follow-up of heart failure. In this report analytical validation data of a non-isotopic point-of-care testing system for the quantitative determination of BNP (Triage BNP, Biosite, USA) are given. Despite a very short turn-around time of about 10 minutes the assay proved to be reproducible (interassay coefficient of variation (n=10) of 8.4% and 8.0% at concentrations of 19.3 ng/l and 392 ng/l, respectively), linear (r=0.998, from 5 ng/l to 818 ng/l), and rugged with respect to common interferents; compared to the widely used SHIONORIA BNP assay (CIS, France) higher results were found (Triage-BNP = 1.52 x SHIONORIA BNP - 7.0 ng/l; n=70) with a relatively close correlation of the results (r=0.935). It is concluded that the Triage BNP assay meets the analytical requirements for further clinical validation and may allow a more widespread clinical use of BNP determination in contrast to competing assays with long turn-around times.
authors.affiliation: Institute of Clinical Chemistry, Ludwig-Maximilians-Universitat Munich-Grosshadern, Germany. Michael.Vogeser@klch.med.uni-muenchen.de
authors.forename: M
authors.initials: M
authors.lastname: Vogeser
authors.forename: K
authors.initials: K
authors.lastname: Jacob
mesh_terms: D009320:Atrial Natriuretic Factor / Q000097:blood*; D002303:Cardiac Output, Low / Q000097:blood / Q000175:diagnosis; D006801:Humans; D016014:Linear Models; D020097:Natriuretic Peptide, Brain; D019095:Point-of-Care Systems; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D020097:Natriuretic Peptide, Brain; D009320:Atrial Natriuretic Factor