title: 25 Hz electromagnetic field exposure has no effect on cell cycle distribution and apoptosis in U-937 and HCA-2/1cch cells.
abstract: It is reported that exposure to 50 Hz extremely low-frequency electromagnetic field (ELF-EMF) can produce apoptosis and small variations in cell cycle distribution on different cell lines. In order to study the effect of ELF-EMF on tumoral cells in vitro, two cell lines (U-937, from a histiocytic lymphoma, and HCA-2/1cch, from a human colon adenocarcinoma) were exposed to 25 Hz, 1.5 mT, for 2 h and 45 min. Cell cycle distribution, apoptosis (spontaneous and dexamethasone-induced) and cell growth were evaluated. Neither significant alteration in cell cycle phases nor induction of apoptosis was observed. Nevertheless, the relative cell number was found to decrease to 55.84+/-7.35% (p <0.05, Student's t-test) for HCA-2/1cch cells after exposure to EMF in the presence of dexamethasone. The presence of dexamethasone during the EMF exposure could probably produce a decrease in the cell growth of this cell line.
authors.affiliation: Laboratorio de Radiobiología, Departamento de Radiologia y Medicina Fisica, Facultad de Medicina, Universidad de Malaga, Spain.
authors.forename: M J
authors.initials: MJ
authors.lastname: Ruiz Gómez
authors.forename: L
authors.initials: L
authors.lastname: De la Peña
authors.forename: J M
authors.initials: JM
authors.lastname: Pastor
authors.forename: M
authors.initials: M
authors.lastname: Martínez Morillo
authors.forename: L
authors.initials: L
authors.lastname: Gil
mesh_terms: D017209:Apoptosis / Q000528:radiation effects*; D002453:Cell Cycle / Q000528:radiation effects*; D002455:Cell Division / Q000528:radiation effects; D003907:Dexamethasone / Q000494:pharmacology; D004574:Electromagnetic Fields / Q000009:adverse effects*; D006801:Humans; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000528:radiation effects; D020298:U937 Cells / Q000528:radiation effects
chemical_list: D003907:Dexamethasone