Amplification methods for the immunolocalization of rare molecules in cells and tissues.
The needs to precisely assign macromolecules to specific locations and domains within tissues and cells and to reveal antigens which are present in low or even in trace amounts, led to the elaboration of a wide spectrum of immunocytochemical amplification procedures. These arise from the successive improvements of tissue preparation techniques, of antigen retrieval procedures and of immunological or non-immunological detection systems. Improvement of detection systems may be the most active in the development of amplification techniques. Since the early work of Coons, in which by the introduction of the indirect technique has started amplifying the signal, different systems have succeeded in increasing the sensitivity of antigens detection. Indeed, amplification techniques such as the multiple antibody layers, the multiple bridges, the enzyme complexes, the avidin-biotin, the silver intensification, and the numerous variations and combinations among these have increased the sensitivity for the detection of scarce tissue antigens. However, as shown by the recent progress carried out with new approaches such as the catalyzed reporter deposition (CARD) and the enhanced polymer one-step staining (EPOS), more efficient methods are still needed. In electron microscopy, few techniques have reached the resolution afforded by the post-embedding immunogold approach. In spite of this and in order to further increase its sensitivity, new probes and novel approaches are allowing combination of the gold marker with the amplification capacity of enzymes afforded by the CARD technique. Immunogold amplification strategies, such as the multiple incubations with the primary antibody and the use of an anti-protein A antibody have also led to enhanced signals displaying the advantages in terms of resolution and possibilities of quantification inherent to the colloidal gold marker.
Mayer
G
G
Département de Pathologie et Biologie Cellulaire, Université de Montréal C.P. 6128, Succ. Centre-ville, Montréal, Quebec H3C 3J7, Canada. moise.bendyan@unmontreal.ca
Bendayan
M
M
D000818:Animals; D000941:Antigens / Q000032:analysis; D001360:Avidin; D001710:Biotin; D005404:Fixatives; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry / Q000379:methods*; D008854:Microscopy, Electron; D012116:Resins, Plant; D016610:Tissue Embedding / Q000379:methods
D000941:Antigens; D005404:Fixatives; D012116:Resins, Plant; D001360:Avidin; D001710:Biotin