Uteroglobin gene transcription: what's the RUSH?
Prolactin enhances progesterone-dependent transcription of the rabbit uteroglobin gene. RUSH transcription factors are implicated in the signal transduction pathway. The RUSH acronym identifies key features of these nuclear phosphoproteins, that is, RING-finger motif, binds the uteroglobin promoter, structurally related to the SWI/SNF family of transcription factors, and helicase-like. Cloned by recognition site screening, RUSH proteins bind to an 85-bp region (-170/-85) of the uteroglobin promoter that was subsequently identified as a novel prolactin-responsive region by promoter deletion analysis. Gel shift and linker-scanning assays further reduced the RUSH target site to -160/-110. A hexameric core of MCWTDK was identified as the RUSH-specific DNA-binding site (-126/-121) by CASTing. This site overlaps authentic HNF3 beta and OCT-1 binding sites. A unique Type IV P-type ATPase that is embedded in the inner nuclear membrane binds the RING domain of RUSH. The conformationally flexible loop portion of this RING-finger binding protein (RFBP) extends into the nucleoplasm to contact euchromatin. The physical association of RFBP with transcriptionally active chromatin supports the speculation that RFBP targets RUSH transcription factors to the active uteroglobin promoter.
Chilton
B S
BS
Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, 3601 4th Street, Lubbock, TX 79430, USA. beverly.chilton@ttmc.ttuhsc.edu
Hewetson
A
A
Devine
J
J
Hendrix
E
E
Mansharamani
M
M
D000818:Animals; D001665:Binding Sites / Q000187:drug effects / Q000235:genetics; D004268:DNA-Binding Proteins / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D004717:Endometrium / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D008954:Models, Biological; D063150:Polycomb Repressive Complex 1; D011374:Progesterone / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D011388:Prolactin / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D011401:Promoter Regions, Genetic / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000502:physiology; D014157:Transcription Factors; D014158:Transcription, Genetic / Q000502:physiology*; D014598:Uteroglobin / Q000235:genetics* / Q000378:metabolism; D016335:Zinc Fingers / Q000187:drug effects / Q000235:genetics
D004268:DNA-Binding Proteins; C104270:SMARCA3 protein, Oryctolagus cuniculus; D014157:Transcription Factors; D011374:Progesterone; D011388:Prolactin; D014598:Uteroglobin; D063150:Polycomb Repressive Complex 1; C080355:RING1 protein, human