[Comparative characteristics of protein G gene in Streptococci of groups C and G isolated from humans].
The treatment of streptococci, groups C and G, with bromocyanogen made it possible to isolate surface G protein, capable of binding human serum albumin (HSA) and polyclonal IgG. In this work the presence of G protein in all staphylococcal strains, groups C and G, is shown. The differences between the strains by the level of expression, molecular weight and functional activity of G protein, extracted from streptococci of groups C and G, permitted the identification of 3 groups of strains, containing the molecules of G protein with different numbers of IgG- and HSA-binding domains: with 3 IgG- and HSA-binding domains, with 2 IgG- and HSA-binding domains and with only 2 IgG-binding domains. Each strain under study expressed only one of the molecule of G protein. The work shows the possibility of the identification of streptococci, groups C and G, by the molecular characteristics of G proteins themselves and their respective coding genes.
Volchek
N A
NA
Research Institute of Experimental Medicine, St. Petersburg, Russia.
Gupalova
T V
TV
Totolian
A A
AA
D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D003488:Cyanogen Bromide; D006801:Humans; D008970:Molecular Weight; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism; D013291:Streptococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
D001426:Bacterial Proteins; C034284:IgG Fc-binding protein, Streptococcus; D012709:Serum Albumin; D003488:Cyanogen Bromide
Sravnitel'naia kharakteristika izoliatov streptokokkov grupp C i G, vydelennykh ot cheloveka, po genu G belka.