title: Calcium inhibits willardiine-induced responses in kainate receptor GluR6(Q)/KA-2.
abstract: A number of studies have demonstrated that willardiine [(S)-1-(2-amino-2-carboxyethyl) pyrimidine-2,4-dione] is a useful agonist for the activation of AMPA/kainate receptors. Here we examine the effect of extracellular calcium on currents evoked by willardiine in HEK 293 cells expressing the GluR6(Q)/KA-2 kainate receptor subunits. At a concentration of 1.8 mM, Ca2+ inhibited the currents induced by 100 microM willardiine by approximately 50%. When extracellular Na+ ions were replaced with Ca2+ ions there were no measurable inward currents. We conclude that Ca2+ inhibition of the willardiine-induced response is concentration dependent.
authors.affiliation: Department of Information Science, Iwate University, Morioka Japan.
authors.forename: T
authors.initials: T
authors.lastname: Fukushima
authors.forename: J
authors.initials: J
authors.lastname: Marshall
authors.forename: K
authors.initials: K
authors.lastname: Sakata
authors.forename: R
authors.initials: R
authors.lastname: Shingai
mesh_terms: D000409:Alanine / Q000031:analogs & derivatives / Q000494:pharmacology*; D002122:Calcium Chloride / Q000494:pharmacology*; D002478:Cells, Cultured / Q000187:drug effects; D006801:Humans; D007668:Kidney; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D011744:Pyrimidinones; D018092:Kainic Acid Receptors / Q000187:drug effects* / Q000502:physiology; D014498:Uracil; D000097806:GluK2 Kainate Receptor
chemical_list: D000409:Alanine; D002122:Calcium Chloride; D011744:Pyrimidinones; D018092:Kainic Acid Receptors; D014498:Uracil; D000097806:GluK2 Kainate Receptor; C002872:willardiine