Ingrowth of sympathetic innervation occurs concomitantly with a decrease of ANP in the growing rat cardiac ventricles.
The relationship between the immunohistochemical expression of atrial natriuretic peptide and the occurrence of sympathetic nerve fibres, as visualized by staining for tyrosine hydroxylase, in the growing rat heart was evaluated. Rats were investigated at four different stages from birth to 21 days postnatally. The effects of chemical destruction of sympathetic nerve terminals in neonatal rats on the cardiac atrial natriuretic peptide content were furthermore examined by use of radioimmunoassay. There was in principle a reciprocal pattern of immunoreaction for atrial natriuretic peptide and tyrosine hydroxylase positive innervation in the ventricular myocardium, atrial natriuretic peptide reaction becoming less and less pronounced with the ingrowth of innervation positive for tyrosine hydroxylase. Furthermore, in the peripheral Purkinje fibre network, there was a marked atrial natriuretic peptide immunoexpression and scarce or no nerve fibres throughout the examination period. The radioimmunoassay measurements showed that chemical sympathectomy lead to elevated cardiac levels of atrial natriuretic peptide. The study shows that sympathetic innervation grows into the ventricular parts concomitantly with the occurrence of a decline in atrial natriuretic peptide expression during development of the heart. Furthermore, it is shown that a reversion of the in growth of sympathetic innervation by destruction of cardiac sympathetic nerves at an early stage leads to increased levels of atrial natriuretic peptide in the heart. The results give new evidence to the phenomenon that the atrial natriuretic peptide levels in the ventricular myocardium and the peripheral parts of the conduction system are under influence of the presence of sympathetic innervation.
Hansson
M
M
Department of Integrative Medical Biology, Umeå University, Sweden. magnus.hansson@anatomy.umu.se
Kjörell
U
U
Forsgren
S
S
D000818:Animals; D000831:Animals, Newborn; D020517:Atrial Appendage / Q000254:growth & development / Q000294:innervation / Q000378:metabolism; D009320:Atrial Natriuretic Factor / Q000378:metabolism*; D001369:Axons / Q000378:metabolism* / Q000648:ultrastructure; D005260:Female; D006329:Heart Conduction System / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D006352:Heart Ventricles / Q000254:growth & development* / Q000294:innervation* / Q000378:metabolism; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D016627:Oxidopamine; D011247:Pregnancy; D011863:Radioimmunoassay; D051381:Rats; D017207:Rats, Sprague-Dawley; D013563:Sympathectomy, Chemical / Q000009:adverse effects; D013564:Sympathetic Nervous System / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D009320:Atrial Natriuretic Factor; D016627:Oxidopamine; D014446:Tyrosine 3-Monooxygenase