VIP- and PACAP-induced salivary chromogranin A secretion in the isolated perfused submandibular gland of rats.
In the study reported in this paper, sensitive ELISA for rat CgA was developed using synthetic rat CgA(359-389) as antigen, N alpha-biotinylated glycylglycyl rat CgA(359-389), and antirat CgA(359-389) serum for the measurement of CgA-LI in rat saliva. CgA-LI in rat submandibular tissues and saliva was characterized by both immunohistochemical and immunochemical methods. Using isolated perfused rat submandibular gland. VIP at 0.1-1.0 nM in the presence of 0.1 microM ACh was found to cause CgA-LI secretion, whereas neither PACAP-27 nor PACAP-38 showed any effect on CgA secretion.
Yanaihara
N
N
Yanaihara Institute Inc., Fujinomiya-shi, 418-0011 Shizuoka, Japan.
Kanno
T
T
Asada
N
N
Iwanaga
T
T
Jun
L
L
Nagasawa
S
S
Yanaihara
C
C
D000595:Amino Acid Sequence; D000818:Animals; D053379:Chromogranin A; D002864:Chromogranins / Q000737:chemistry / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000378:metabolism / Q000494:pharmacology*; D010446:Peptide Fragments / Q000737:chemistry; D010477:Perfusion; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D051381:Rats; D012463:Saliva / Q000378:metabolism; D013363:Submandibular Gland / Q000187:drug effects* / Q000378:metabolism*; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism / Q000494:pharmacology*
C495403:Adcyap1 protein, rat; D053379:Chromogranin A; D002864:Chromogranins; D009479:Neuropeptides; D010446:Peptide Fragments; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; C506153:chromogranin A, rat; D014660:Vasoactive Intestinal Peptide