[Detection of impurities in DNA samples by using the convolution spectrum method].
OBJECTIVE
To detect the presence of phenol, amino acids and endotoxin in DNA preparations.


METHODS
By using the convolution curve transformation technology, correlation coefficient discriminant which was put forward by spectrum space theory and computer information process technology, impurities in DNA samples were detected.


RESULTS
The convolution spectrum method compared with A260/A280 ratio method and second-derivative absorption spectrum method could be used for the semiquantitative determinations of impurities in DNA samples and the results were satisfactory.


CONCLUSIONS
The method was simple, convenient, fast and valuable to the biochemical analysis.
Zheng
H
H
Department of Pharmaceutical Analysis, College of Pharmacy, Second Military Medical University, Shanghai 200433, China.
Wu
Y T
YT
Fang
H S
HS
D000596:Amino Acids / Q000032:analysis*; D004247:DNA / Q000032:analysis*; D004731:Endotoxins / Q000032:analysis*; D019800:Phenol / Q000032:analysis*; D013057:Spectrum Analysis / Q000379:methods
D000596:Amino Acids; D004731:Endotoxins; D019800:Phenol; D004247:DNA