Cytotoxicity of dental composite components and mercury compounds in pulmonary cells.
The cytotoxic potentials of the dental composite components triethyleneglycoldimethacrylate (TEGDMA) and 2-hydroxy-ethylmethacrylate (HEMA) as well as mercuric chloride (HgCl2) and methyl mercury chloride (MeHgCl) were investigated. Proliferating A549 and L2 cell monolayers were cultured in the absence or presence of composite components or mercurials. Twenty-four hours later the tetrazolium salt XTT (sodium 3'-[1-phenyl-aminocarbonyl)-3,4-tetrazolium]bis(4-methoxy-6-nitro)benzenesulphonic acid) was added. Formazan formation was quantified using a microtiter plate reader. EC50 values were obtained as half-maximum-effect concentrations from fitted curves. EC50 values were in A549 cells (mean values +/- standard deviation; n = 12; micromol/l); HEMA 8854+/-1882; TEGDMA 1821+/-529; HgCl2 41+/-7 and MeHgCl 27+/-3. EC50 values in L2 cells were: HEMA 191+/-28; TEGDMA 112+/-16; HgCl2 25+/-6 and MeHgCl 8+/-6. All tested substances induced a dose-dependent loss of viability in A549 and L2 cells after 24 h. The EC50 values of both mercurials were significantly (p < 0.05) lower compared to the values of both composite components. TEGDMA was about 5-fold (A549 cells) and about 2-fold (L2 cells) more toxic compared to HEMA. It is to be assumed that the risk of lung cell damage by dental composite components is even more unlikely.
Kehe
K
K
Walther-Straub Institute of Pharmacology and Toxicology, Ludwig-Maximilians University of Munich, Germany. kai.kehe@lrz.uni-muenchen.de
Reichl
F X
FX
Durner
J
J
Walther
U
U
Hickel
R
R
Forth
W
W
D000180:Acrylic Resins / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D003188:Composite Resins / Q000494:pharmacology*; D006801:Humans; D008168:Lung / Q000166:cytology / Q000187:drug effects*; D017669:Mercury Compounds / Q000494:pharmacology*; D008689:Methacrylates / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000494:pharmacology*; D011109:Polymethacrylic Acids / Q000494:pharmacology*; D011140:Polyurethanes / Q000494:pharmacology*; D051381:Rats; D014407:Tumor Cells, Cultured
D000180:Acrylic Resins; C029012:Composite Dental Resin; D003188:Composite Resins; D017669:Mercury Compounds; D008689:Methacrylates; D011109:Polymethacrylic Acids; D011140:Polyurethanes; C020946:triethylene glycol dimethacrylate; D011092:Polyethylene Glycols; C005044:hydroxyethyl methacrylate