Postantibiotic effects of gentamicin and netilmicin on Serratia marcescens: effects on hydrophobicity and motility.
The impact of postantibiotic effect (PAE) of aminoglycosides (gentamicin, netilmicin) on cell-surface hydrophobicity and motility of a clinical isolate Serratia marcescens was evaluated. For the induction of PAE 2x and 4xMIC concentrations of both antibiotics were used. Gentamicin and netilmicin induced a PAE of similar duration after 2xMIC concentration (2.7 and 2.8 h, respectively). Both aminoglycosides demonstrated concentration-dependent PAE. At a concentration of 4xMIC they produced PAEs of 5.9 and 8.2 h, respectively. The evaluation of hydrophobic properties of S. marcescens after affecting PAE showed that both aminoglycosides inhibited adherence to xylene. This inhibition was also concentration-dependent. More expressive was netilmicin which inhibited the adhesion by 70.5% at 2xMIC and by 85.2% at 4xMIC. Netilmicin inhibited also the adhesion to nitrocellulose filter by 34.7% at 4xMIC. Exposure of the bacterial cells to suprainhibitory concentrations of both aminoglycosides resulted only in moderate inhibition of motility of strain tested compared to the unexposed cells.
Majtánová
L
L
Institute of Preventive and Clinical Medicine, 833 01 Bratislava 37, Slovakia.
Majtán
V
V
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005839:Gentamicins / Q000494:pharmacology*; D006801:Humans; D009068:Movement; D009428:Netilmicin / Q000494:pharmacology*; D012706:Serratia marcescens / Q000187:drug effects* / Q000502:physiology
D000900:Anti-Bacterial Agents; D005839:Gentamicins; D009428:Netilmicin