title: Adhesion of human osteoblasts on root-end filling materials.
abstract: Adhesion of human osteoblasts to root-end filling materials (mineral trioxide aggregate (MTA), IRM, composite, and amalgam) was observed by scanning electron microscopy. Root-end filling materials were inserted into 96-well flat-bottomed plates and condensed to disks of approximately 1 mm thick and the same diameter as the wells. After the disks were set, they were placed in the bottom of Nunc four-well culture plates at one disk per well. Then human osteoblasts were seeded into the wells at 1.5 x 10(5) cells per well. After 1 day in culture the disks of root-end filling materials along with cells grown on their surface were examined with a scanning electron microscopy. Results showed that osteoblasts attached and spread on MTA and composite by forming a monolayer. Osteoblasts also attached on amalgam, but with few cells spreading. In the presence of IRM, osteoblasts appeared rounded with no spreading. These results indicate that osteoblasts have a favorable response to MTA and composite resin compared with IRM and amalgam.
authors.affiliation: Department of Endodontology, School of Dental Medicine, University of Connecticut Health Center 06030-1715, USA.
authors.forename: Q
authors.initials: Q
authors.lastname: Zhu
authors.forename: R
authors.initials: R
authors.lastname: Haglund
authors.forename: K E
authors.initials: KE
authors.lastname: Safavi
authors.forename: L S
authors.initials: LS
authors.lastname: Spangberg
mesh_terms: D017607:Aluminum Compounds / Q000494:pharmacology; D017610:Calcium Compounds / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects* / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D003723:Dental Amalgam / Q000494:pharmacology; D004338:Drug Combinations; D006801:Humans; D008422:Materials Testing; D008768:Methylmethacrylates / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000187:drug effects* / Q000502:physiology; D010087:Oxides / Q000494:pharmacology; D012184:Retrograde Obturation; D012387:Root Canal Filling Materials / Q000737:chemistry / Q000494:pharmacology*; D017640:Silicates / Q000494:pharmacology; D013499:Surface Properties; D015035:Zinc Oxide-Eugenol Cement / Q000494:pharmacology
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D004338:Drug Combinations; D008768:Methylmethacrylates; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; D015035:Zinc Oxide-Eugenol Cement; C086631:mineral trioxide aggregate; C031667:IRM cement; D003723:Dental Amalgam