title: Effect of retinoids on growth inhibition of two canine melanoma cell lines.
abstract: Two new canine melanoma cell lines (CMM1 and CMM2) were established from the patients with oral malignant melanomas. Histopathological type of both CMM1 and CMM2 was a mixed cell type consisted of spindle-shaped cells, polygonal cells, and oval cells. Doubling time of CMMI and CMM2 were 18.4 +/- 1.96 hr and 21.0 +/- 0.73 hr, respectively. The effect of two kinds of retinoids (all-trans retinoic acid and 9-cis retinoic acid) on the proliferation of these cells were examined by morphological changes, proliferation assay and apoptosis assay. However, the retinoids did not suppress growth rate of these cells. This result suggests that retinoids used in this study did not induce differentiation, apoptosis, and growth inhibition of the canine melanoma cell lines.
authors.affiliation: Division of Veterinary Medical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Ohashi
authors.forename: S H
authors.initials: SH
authors.lastname: Hong
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
authors.forename: T
authors.initials: T
authors.lastname: Nakagawa
authors.forename: M
authors.initials: M
authors.lastname: Mochizuki
authors.forename: R
authors.initials: R
authors.lastname: Nishimura
authors.forename: N
authors.initials: N
authors.lastname: Sasak
mesh_terms: D000077556:Alitretinoin; D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D008545:Melanoma / Q000473:pathology / Q000662:veterinary*; D009062:Mouth Neoplasms / Q000473:pathology / Q000662:veterinary; D012176:Retinoids / Q000633:toxicity*; D014212:Tretinoin / Q000633:toxicity; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D012176:Retinoids; D000077556:Alitretinoin; D014212:Tretinoin