[Studies of biochemical markers of four isonucleus-alloplasmic lines of common wheat].
The peroxidase isozyme and the soluble peoteins of four isonucleus-alloplasmic lines of common wheat--D2-CA8057 (fertile, BC14), CMS line msD2-CA8057(BC11), CMS line msA-CA8057(BC12), CA8057 (nucleus donor) in different developmental stages were studies by polyacrylamide gel electrophoresis (PAGE) and gradient SDS-PAGE respectively. The peroxidase isozyme of bionucleus-stage anther, milk-stage endosperm and the soluble proteins of bionucleus-stage anther, leaf (the beginning and ending overwinter), dry-seed endosperm among fertile line, msD2-CA8057 and msA-CA8057 were evidently different (but few differences between CA8057 and D2-CA8057). These differences resulted from the pecially-regulated expression of (male-sterile) cytoplasmic genes to nuclear genes and could be used to identify the four isonucleus-alloplasmic lines. These results showed that msD2-CA8057 and msA-CA8057 were two different kinds of new CMS pattern.
Liu
C G
CG
State Key Laboratory of Plant Cell Chromosome Engineering, Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China.
Wu
Y W
YW
Hou
N
N
Zhang
C L
CL
Zhang
Y
Y
Li
X M
XM
Li
Z C
ZC
Yao
R
R
D015415:Biomarkers; D007527:Isoenzymes / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism*; D010940:Plant Proteins / Q000032:analysis*; D012098:Reproduction; D014908:Triticum / Q000235:genetics / Q000378:metabolism*
D015415:Biomarkers; D007527:Isoenzymes; D010940:Plant Proteins; D010544:Peroxidases