The use of dominant negative mutants to study the function of mitotic motors in the in vitro spindle assembly assay in Xenopus egg extracts.
Boleti
H
H
Institut Pasteur, Unité de Biologie des Interactions Cellulaires, Paris, France.
Karsenti
E
E
Vernos
I
I
D000818:Animals; D015153:Blotting, Western; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D002474:Cell-Free System; D005260:Female; D005307:Fertilization in Vitro; D005799:Genes, Dominant; D005978:Glutathione / Q000235:genetics; D016547:Kinesins / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D008297:Male; D008677:Metaphase; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000737:chemistry / Q000502:physiology; D008938:Mitosis; D020409:Molecular Motor Proteins / Q000235:genetics / Q000502:physiology*; D009068:Movement; D009865:Oocytes / Q000648:ultrastructure; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D013048:Specimen Handling / Q000379:methods; D008941:Spindle Apparatus / Q000502:physiology*; D029867:Xenopus Proteins; D014982:Xenopus laevis
D018797:Cell Cycle Proteins; C097723:KIF15 protein, Xenopus; D020409:Molecular Motor Proteins; D011993:Recombinant Fusion Proteins; D029867:Xenopus Proteins; D016547:Kinesins; D005978:Glutathione