Anti-alpha B-crystallin immunoreactivity in inflammatory nervous system diseases.
alpha B-Crystallin, a small heat shock protein, is an immunodominant antigen with increased tissue expression in demyelination. To investigate the humoral response against alpha B-crystallin, the sera and CSF samples of patients with multiple sclerosis (MS), Guillain-Barré syndrome (GBS), neuro-Behçet's disease (NBD) and other non-inflammatory neurological diseases (NIND) were screened by enzyme-linked immunosorbent assay for anti-alpha B-crystallin IgG and IgM antibodies. Serum and CSF IgG antibody responses to alpha B-crystallin were significantly elevated only in NBD patients (serum IgG, NBD 1.29 +/- 0.49 vs. NIND 0.95 +/- 0.39, P = 0.01; CSF IgG, NBD 1.22 +/- 0.64 vs. NIND 0.81 +/- 0.35, P = 0.01). Similarly, high serum IgM antibody titres were also detected in NBD (1.83 +/- 0.72 vs. 1.16 +/- 0.49, P = 0.0005) and in MS (1.57 +/- 1.07, P = 0.046), whereas elevated CSF IgM responses were observed only in GBS (2.09 +/- 1.09 vs. 1.41 +/- 0.7, P = 0.007). Humoral responses against alpha B-crystallin are increased in NBD and GBS, which may implicate this central nervous system antigen in the causation and pathogenesis of these inflammatory nervous system disorders.
Celet
B
B
Department of Physiology, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey.
Akman-Demir
G
G
Serdaroğlu
P
P
Yentür
S P
SP
Taşci
B
B
van Noort
J M
JM
Eraksoy
M
M
Saruhan-Direskeneli
G
G
D000328:Adult; D001323:Autoantibodies / Q000097:blood / Q000134:cerebrospinal fluid; D001426:Bacterial Proteins; D001528:Behcet Syndrome / Q000276:immunology* / Q000378:metabolism; D018834:Chaperonin 60; D018833:Chaperonins / Q000032:analysis / Q000276:immunology; D003459:Crystallins / Q000032:analysis* / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D020275:Guillain-Barre Syndrome / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000134:cerebrospinal fluid; D007075:Immunoglobulin M / Q000097:blood / Q000134:cerebrospinal fluid; D008297:Male; D009103:Multiple Sclerosis / Q000276:immunology* / Q000378:metabolism
D001323:Autoantibodies; D001426:Bacterial Proteins; D018834:Chaperonin 60; D003459:Crystallins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; C074119:heat-shock protein 65, Mycobacterium; D018833:Chaperonins