[Growth of Escherichia coli cell culture and frequency of mutation, induced by N-methyl-N'-nitro-N-nitrosoguanidine].
The rate of Escherichia coli mutation with N-methyl-N'-nitro-N-nitrosoguanidine was found to depend on the age of the culture and the pH value of the incubation medium. The mutability of late-logarithmic cells was higher than that of mid-logarithmic cells. Rifampicin, a transcription inhibitor, enhanced the mutagenic action of MNNG on cells from the late logarithmic and early stationary phases suspended in a medium with a pH of 7.4, and on cells from the mid-logarithmic phase suspended in a medium with a pH of 6.5. Conversely, streptomycin decreased the frequency of induced mutations in cells from the late logarithmic and early stationary phases suspended in the medium with a pH of 7.4, and in cells from the mid-logarithmic and early stationary phases suspended in the medium with a pH of 6.5. These data were interpreted as showing that the repair of premutational DNA lesions induced by MNNG depends on the level of DNA transcription and translation.
Goliasnaia
N V
NV
Institute of Ecology and Genetics of Microorganisms, Ural Division, Russian Academy of Sciences, ul. Pushkina 1-57, Perm, 614000 Russia.
Tiurina
N A
NA
D003470:Culture Media; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D006863:Hydrogen-Ion Concentration; D008769:Methylnitronitrosoguanidine / Q000633:toxicity*; D009153:Mutagens / Q000633:toxicity*; D009154:Mutation; D012293:Rifampin / Q000494:pharmacology
D003470:Culture Media; D009153:Mutagens; D008769:Methylnitronitrosoguanidine; D012293:Rifampin
Vozrast kul'tury kletok Escherichia coli i chastota mutatsiĭ, indutsirovannykh N-metil-N'-nitro-N-nitrozoguanidinom.