Immune complex processing in C1q-deficient mice.
Complement and Fcgamma receptors are known to mediate the processing of immune complexes (IC), and abnormalities in these mechanisms may predispose to the development of lupus. We explored the processing of IC in mice deficient in complement component C1q. 125I-labelled IC comprising Hepatitis B surface antigen (HBsAg)/human anti-HBsAg (HBsAg/Ab) were injected intravenously and the sites of IC clearance determined by direct counting of organ uptake at various time points. The liver and spleen were the main sites of IC uptake in all mice. The splenic uptake of IC was significantly reduced in the C1q-deficient mice compared with the control mice. C1q-deficient mice also exhibited an initial accelerated hepatic uptake of IC similar to that seen in human subjects with hypocomplementaemia. The hepatic localization of IC at later time points was similar in both groups of mice. These data in mice are consistent with previous observations in humans that confirm that the classical pathway of complement plays an important role in the appropriate processing of IC in vivo.
Nash
J T
JT
Rheumatology Section, Division of Medicine, Imperial College School of Medicine, London, UK.
Taylor
P R
PR
Botto
M
M
Norsworthy
P J
PJ
Davies
K A
KA
Walport
M J
MJ
D000818:Animals; D000936:Antigen-Antibody Complex / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D015922:Complement C1q / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D017353:Gene Deletion; D005786:Gene Expression Regulation / Q000276:immunology; D006801:Humans; D008177:Lupus Vulgaris / Q000209:etiology / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D051379:Mice
D000936:Antigen-Antibody Complex; D015922:Complement C1q