Expression, purification, and biochemical properties of Ypt/Rab GTPase-activating proteins of Gyp family.
Will
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Department of Molecular Genetics, Max Planck Institute for Biophysical Chemistry, Göttingen D-37070, Germany.
Albert
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S
Gallwitz
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D020134:Catalytic Domain; D002846:Chromatography, Affinity / Q000379:methods; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004926:Escherichia coli; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D020690:GTPase-Activating Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D020691:rab GTP-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*
D005656:Fungal Proteins; D020690:GTPase-Activating Proteins; C416529:GYP1 protein, S cerevisiae; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D006160:Guanosine Triphosphate; C060755:YPT1 protein, S cerevisiae; D020691:rab GTP-Binding Proteins