title: Modified short-term guinea pig sensitization tests for detecting contact allergens as an alternative to the conventional test.
abstract: The conventional adjuvant and patch test (APT) method of guinea pig sensitization testing was modified in 2 ways, s-APT and s-APT(2), in order to shorten the test period. These short-term test methods consist of 72-h closed application of test material with intradermal injection of emulsified Freund's complete adjuvant (E-FCA) for 1st induction, 48-h closed application of test material with (s-APT) or without (s-APT(2)) intradermal injection of E-FCA on the 7th day for 2nd induction, and open application on the 14th day for challenge. They were compared with conventional APT by using 8 allergenic chemicals (formaldehyde, nickel sulfate, cobalt sulfate, ethyl-p-aminobenzoate (benzocaine), isoeugenol, 2-mercaptobenzothiazole, 2,4-dinitrochlorobenzene (DNCB) and 1-phenylazo-2-naphthol (Sudan I)). The short-term methods gave similar results to those of conventional APT in terms of mean response, sensitization rate and sensitization potency (challenge concentration that induces a mean response equal to 1.0). Thus, our short-term methods, which are capable of evaluating skin sensitization within 17 days, are sufficiently sensitive to detect potentially hazardous contact allergens.
authors.affiliation: Life Science Research Center, Shiseido Corporation, Ltd., Yokohama, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Yanagi
authors.forename: M
authors.initials: M
authors.lastname: Hoya
authors.forename: M
authors.initials: M
authors.lastname: Mori
authors.forename: Y
authors.initials: Y
authors.lastname: Katsumura
mesh_terms: D000485:Allergens; D000818:Animals; D017449:Dermatitis, Allergic Contact / Q000175:diagnosis*; D004195:Disease Models, Animal; D005260:Female; D006168:Guinea Pigs; D010328:Patch Tests / Q000379:methods
chemical_list: D000485:Allergens