Protein-based chiral stationary phases for high-performance liquid chromatography enantioseparations.
The enantioseparations of various compounds using proteins as the chiral selectors in high-performance liquid chromatography (HPLC) are considered in this review. The proteins used include albumins such as bovine serum albumin and human serum albumin, glycoproteins such as alpha1-acid glycoprotein, ovomucoid, ovoglycoprotein, avidin and riboflavin binding protein, enzymes such as trypsin, alpha-chymotrypsin, cellobiohydrolase I, lysozyme, pepsin and amyloglucosidase, and other proteins such as ovotransferrin and beta-lactoglobulin. This review deals with the properties of HPLC chiral stationary phases based on proteins, and the enantioselective properties and chiral recognition mechanisms of these stationary phases.
Haginaka
J
J
Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Hyogo, Japan. haginaka@mwu.mukogawa-u.ac.jp
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D011506:Proteins / Q000737:chemistry*; D013237:Stereoisomerism
D011506:Proteins