A morphological study of the expression of the small G protein RhoA in resting and activated MDCK cells.
The small G protein Rho subfamily controls several cellular events such as growth, movement, proliferation and differentiation by rearranging actin and cytoskeleton proteins. Most of these effects are mediated by the activation of growth factor and extracellular matrix molecule receptors, suggesting a role for Rho molecules in the transduction pathway of these receptors. Despite the importance of Rho peptides in fundamental cellular events, data on their subcellular immunolocalisation are sparse: here we investigated the expression and subcellular localisation of RhoA in resting (cultured on plastic) and activated (Matri-cell or hepatocyte growth factor) MDCK cells by immunoperoxidase and immunogold techniques. Resting MDCK cells contain detectable amounts of RhoA mainly localised in the cytoplasm; RhoA expression is significantly enhanced by Matri-cell substrates that promote translocation of RhoA at the membrane level. This enhancing effect is reduced after exposure to hepatocyte growth factor.
Mattii
L
L
Dipartimento di Morfologia Umana e Biologia Applicata, Sezione di Istologia ed Embriologia Medica, Facoltà di Medicina e Chirurgia, Università di Pisa, Italy.
Bianchi
F
F
Pellegrini
S
S
Dolfi
A
A
Bernardini
N
N
D000818:Animals; D015153:Blotting, Western; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D048429:Cell Size; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D004285:Dogs; D017228:Hepatocyte Growth Factor / Q000494:pharmacology; D007091:Image Processing, Computer-Assisted; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron; D021381:Protein Transport / Q000187:drug effects; D020742:rhoA GTP-Binding Protein / Q000378:metabolism*
D017228:Hepatocyte Growth Factor; D020742:rhoA GTP-Binding Protein