[The use of polymerase chain reaction in the diagnosis of diphtheria infection].
624 Corynebacterium diphtheriae strains, newly isolated from patients and carriers, were studied with the use of the methods of gel immunodiffusion (Elek's test) and polymerase chain reaction (PCR). In the evaluation of 388 C. diptheriae strains, found to be toxigenic in PCR, the results of Elek's test coincided with those of PCR on 98% of cases. In 38 out of 143 strains (26.5%), nontoxigenic according to the results of Elek's test, the presence of the A-fragment of the tox-gene was established. Subculturing in nutrient media made it possible to determine the presence of toxin in 19 out of 38 of these strains; the remaining strains, isolated mainly from carriers, were found to have the "silent" gene. The advantage of using PCR for the detection of toxigenic and nontoxigenic C. diphtheriae strains of different origin was shown.
Tseneva
G Ia
GIa
Pasteur Research Institute of Epidemiology and Microbiology, St. Retersburg.
Shchederkina
E E
EE
D003353:Corynebacterium diphtheriae / Q000302:isolation & purification*; D004165:Diphtheria / Q000175:diagnosis*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
Primenenie polimeraznoĭ tsepnoĭ reaktsii v diagnostike difteriĭnoĭ infektsii.