[Features of formation of phases of dual-phase polymeric system in the presence of collagen I, laminin I, and their mixtures].
A study was made of the phase formation kinetics of a two-phase aqueous polymer system, consisting of 7.8% (w/w) dextran-500 and 4.6% (w/w) polyethylene glycol-6000, in the presence of various concentrations of collagen (0.07-0.20 mg/ml), laminin I (0.01-0.03 mg/ml) and their mixture. The phase formation was evaluated by registration of its optical density on a spectrophotometer. The obtained two-phase polymer system optical density curves and also the partitioning coefficients for the studied objects depend on surface properties of these objects. It has been shown that the surface properties of collagen I and laminin I differ according to differences in their molecules conformations, and that the phase formation kinetics points to their interaction during a mutual partitioning of these proteins in the system. The authors made a conclusion that collagen I and laminin I in the two-phase polymer system conditions could make complexes.
Voronkina
I V
IV
Institute of Cytology RAS, St. Petersburg.
Kukhareva
L V
LV
Petrov
Iu P
IuP
Pinaev
G P
GP
D001704:Biopolymers / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D007797:Laminin / Q000737:chemistry*
D001704:Biopolymers; D007797:Laminin; D003094:Collagen
Osobennosti formirovaniia faz dvukhfaznoĭ polimernoĭ sistemy v prisutstvii kollagena I, lamnina I i ikh smesi.