title: Association of mouse mammary tumor virus superantigen with MHC class II during biosynthesis.
abstract: Mouse mammary tumor viruses encode superantigens that interact with MHC class II proteins and stimulate T cells. We show here that presentation of mouse mammary tumor virus superantigen does not require DM. Furthermore, we have identified a strong class II peptide binding motif in the Mtv-7 superantigen, and we show that this motif is necessary for association with class II molecules in in vitro translation and in vivo functional assays. Our results suggest that endogenously synthesized viral superantigen can bind to MHC class II heterodimers during biosynthesis in the endoplasmic reticulum in a manner analogous to that used by the class II-associated invariant chain.
authors.affiliation: Program in Immunology, Department of Pathology, Tufts University School of Medicine, Boston, MA 02111, USA.
authors.forename: P N
authors.initials: PN
authors.lastname: Hsu
authors.forename: P
authors.initials: P
authors.lastname: Wolf Bryant
authors.forename: N
authors.initials: N
authors.lastname: Sutkowski
authors.forename: B
authors.initials: B
authors.lastname: McLellan
authors.forename: H L
authors.initials: HL
authors.lastname: Ploegh
authors.forename: B T
authors.initials: BT
authors.lastname: Huber
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D017951:Antigen Presentation / Q000235:genetics; D000944:Antigens, Differentiation, B-Lymphocyte / Q000235:genetics; D000956:Antigens, Viral; D002474:Cell-Free System / Q000276:immunology; D019281:Dimerization; D004285:Dogs; D006681:HLA-D Antigens / Q000378:metabolism*; D015798:HLA-DR1 Antigen / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008324:Mammary Tumor Virus, Mouse / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D018448:Models, Immunological; D009154:Mutation; D009842:Oligopeptides / Q000276:immunology / Q000378:metabolism; D011485:Protein Binding / Q000276:immunology; D014176:Protein Biosynthesis / Q000276:immunology; D011817:Rabbits; D018089:Superantigens / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*
chemical_list: D000944:Antigens, Differentiation, B-Lymphocyte; D000956:Antigens, Viral; D006681:HLA-D Antigens; C084245:HLA-DM antigens; D015798:HLA-DR1 Antigen; D000949:Histocompatibility Antigens Class II; D008562:Membrane Glycoproteins; C095378:Mtv-7 superantigen, Mouse mammary tumor virus; D009842:Oligopeptides; D018089:Superantigens; C045084:invariant chain