Detection of Bartonella henselae and Afipia felis DNA by polymerase chain reaction in specimens from patients with cat scratch disease.
Polymerase chain reaction (PCR) amplification and colorimetric identification of amplicons were performed to detect Bartonella henselae and Afipia felis DNA in specimens from patients who were clinically and histologically suspected of having cat scratch disease. PCR products were revealed using 2% ethidium bromide agarose-gel electrophoresis and identified with specific probes in a commercial colorimetric hybridization assay (DEIA) (GEN-ETI-K; DiaSorin, Italy). Six paraffin-embedded lymph node biopsies from 18 patients as well as 18 samples of peripheral whole blood and 18 sera were investigated. Bartonella henselae DNA was recovered from the whole blood of four patients, and Bartonella henselae and Afipia felis DNA were detected in one patient's lymph node biopsy. This study suggests that PCR-DEIA is sufficiently sensitive to be considered feasible for the molecular diagnosis of cat scratch disease.
Del Prete
R
R
Dipartimento di Clinica Medica, Immunologia e Malattie Infettive, Università degli Studi di Bari, Italy. rdelprete@midim.uniba.it
Fumarola
D
D
Fumarola
L
L
Miragliotta
G
G
D000293:Adolescent; D000328:Adult; D020622:Afipia / Q000235:genetics / Q000302:isolation & purification*; D018416:Bartonella henselae / Q000235:genetics / Q000302:isolation & purification*; D002372:Cat-Scratch Disease / Q000175:diagnosis* / Q000382:microbiology; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis / Q000097:blood*; D005260:Female; D006801:Humans; D008198:Lymph Nodes / Q000382:microbiology; D008297:Male; D009693:Nucleic Acid Hybridization / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*
D004269:DNA, Bacterial