Determination of vitamins A and E in milk samples by fluorescence in micellar media.
The fat-soluble vitamins A and E in milk samples were determined by fluorescence at room temperature in an aqueous media of micellar solutions. Different types of surfactants were studied; the cationic hexadecyltrimethylammonium bromide (CTAB), the anionic sodium dodecylsulfate (SDS) and the non-ionic polyoxyethylene(23)laurylether (Brij 35). The detection limits ranged between 50 and 90 ng.L-1 for both vitamins in CTAB and Brij 35. The method has been applied to the determination of vitamins A and E in milk samples.
Ramos-Lledó
P
P
Dpto. Química Analítica, Facultad de Química, Universidad de Alcalá, 28871 Alcalá de Henares, Spain.
Vera
S
S
San Andrés
M P
MP
D000818:Animals; D000077286:Cetrimonium; D002593:Cetrimonium Compounds; D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel; D007202:Indicators and Reagents; D008823:Micelles; D008892:Milk / Q000737:chemistry*; D000077423:Polidocanol; D011092:Polyethylene Glycols; D013050:Spectrometry, Fluorescence; D014801:Vitamin A / Q000032:analysis*; D014810:Vitamin E / Q000032:analysis*
D002593:Cetrimonium Compounds; D003902:Detergents; D007202:Indicators and Reagents; D008823:Micelles; D000077423:Polidocanol; D014801:Vitamin A; D014810:Vitamin E; D011092:Polyethylene Glycols; D000077286:Cetrimonium