Electron microscopy of the K2 killer effect of Saccharomyces cerevisiae T206 on a mesophilic wine yeast.
A mesophilic wine yeast, Saccharomyces cerevisiae CSIR Y217 K-R- was subjected to the K2 killer effect of Saccharomyces cerevisiae T206 K+R+ in a liquid grape medium. The lethal effect of the K2 mycoviral toxin was confirmed by methylene blue staining. Scanning electron microscopy of cells from challenge experiments revealed rippled cell surfaces, accompanied by cracks and pores, while those unaffected by the toxin, as in the control experiments, showed a smooth surface. Transmission electron microscopy revealed that the toxin damaged the cell wall structure and perturbed cytoplasmic membranes to a limited extent.
Vadasz
A S
AS
Department of Biochemistry, University of Durban-Westville, Durban, South Africa.
Jagganath
D B
DB
Pretorius
I S
IS
Gupthar
A S
AS
D000898:Antibiosis; D002473:Cell Wall / Q000648:ultrastructure; D004396:Coloring Agents; D003470:Culture Media; D005285:Fermentation; D005656:Fungal Proteins / Q000378:metabolism*; D007425:Intracellular Membranes / Q000648:ultrastructure; D055646:Killer Factors, Yeast; D008751:Methylene Blue; D008855:Microscopy, Electron, Scanning; D009183:Mycotoxins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000648:ultrastructure*; D029701:Saccharomyces cerevisiae Proteins; D014920:Wine / Q000382:microbiology*
D004396:Coloring Agents; D003470:Culture Media; D005656:Fungal Proteins; C073585:K2 killer toxin; D055646:Killer Factors, Yeast; D009183:Mycotoxins; D029701:Saccharomyces cerevisiae Proteins; D008751:Methylene Blue