Application of reinforced frontal affinity chromatography and advanced processing procedure to the study of the binding property of a Caenorhabditis elegans galectin.
Frontal affinity chromatography is a very useful and simple method to analyze molecular interactions between an analyte and an immobilized ligand by calculating the extent of "retardation" of the elution front. We developed a very simple and efficient data-processing procedure that enables the measurement of very small differences in retardation with precision. This procedure was successfully applied to comparison of the binding properties of recombinant C. elegans galectins for their ligand.
Arata
Y
Y
Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan. y-arata@pharm.teikyo-u.ac.jp
Hirabayashi
J
J
Kasai
K I
KI
D000818:Animals; D017173:Caenorhabditis elegans / Q000378:metabolism*; D002846:Chromatography, Affinity / Q000379:methods*; D037161:Galectins; D006388:Hemagglutinins / Q000378:metabolism*; D011485:Protein Binding; D011994:Recombinant Proteins / Q000378:metabolism
D037161:Galectins; D006388:Hemagglutinins; D011994:Recombinant Proteins