High-performance liquid chromatographic separation of enantiomers of synthetic amino acids on a ristocetin A chiral stationary phase.
A macrocyclic glycopeptide, ristocetin A, was used as chiral stationary phase for the high-performance liquid chromatographic separation of enantiomers of 28 unnatural amino acids, such as analogues of phenylalanine, tyrosine and tryptophan, and analogues containing 1,2,3,4-tetrahydroisoquinoline, tetraline or 1,2,3,4-tetrahydro-2-carboline skeletons. Excellent resolutions were achieved for most of the investigated compounds by using reversed-phase or a new polar-organic mobile phase system. The conditions of separation were optimized by variation of the mobile phase composition, temperature and flow-rate.
Péter
A
A
Department of Inorganic and Analytical Chemistry, University of Szeged, Hungary. apeter@chem.u-szeged.hu
Török
G
G
Armstrong
D W
DW
Tóth
G
G
Tourwé
D
D
D000596:Amino Acids / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D012310:Ristocetin / Q000737:chemistry*; D013237:Stereoisomerism
D000596:Amino Acids; D012310:Ristocetin; C016719:ristocetin A