title: Probing the MVAI methyltransferase region that interacts with DNA: affinity labeling with the dialdehyde-containing DNA duplexes.
abstract: Affinity labeling of methyltransferase MvaI by DNA duplexes containing oxidized 2'-O-beta-D-ribofuranosylcytidine or 1-beta-D-galactopyranosyl)thymine residues was performed. Partial chemical hydrolysis of the covalently bound methylase in the conjugates with the dialdehyde-containing DNA allowed us to determine the amino acid region in the C terminus of methylase MvaI that interacts with DNA.
authors.affiliation: Department of Chemistry, Moscow State University, Russia.
authors.forename: O M
authors.initials: OM
authors.lastname: Gritsenko
authors.forename: S N
authors.initials: SN
authors.lastname: Mikhailov
authors.forename: E V
authors.initials: EV
authors.lastname: Efimtseva
authors.forename: A
authors.initials: A
authors.lastname: Van Aerschot
authors.forename: P
authors.initials: P
authors.lastname: Herdewijn
authors.forename: E S
authors.initials: ES
authors.lastname: Gromova
mesh_terms: D000345:Affinity Labels; D000447:Aldehydes / Q000737:chemistry*; D004247:DNA / Q000378:metabolism*; D019175:DNA Methylation; D015257:DNA-Cytosine Methylases / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D015335:Molecular Probes; D013379:Substrate Specificity
chemical_list: D000345:Affinity Labels; D000447:Aldehydes; D015335:Molecular Probes; D004247:DNA; C029528:DNA modification methylase EcoRII; D015257:DNA-Cytosine Methylases