title: Neonatal and adult human heart tissues from normal subjects and patients with ischemic, dilated or hypertrophic cardiomyopathy express insulin-like growth factor binding protein-3 (IGFBP-3).
abstract: In the human fetus IGFBP-3 mRNA expression is most abundant in the skin, muscle and heart but circulating IGFBP-3 levels show age-related variations. In human heart tissues from controls and patients with either ischemic, dilated or hypertrophic cardiomyopathy (no.: 20, age-range from fetuses to elderly subjects) we determined the expression of cardiac IGFBP-3 mRNA by reverse transcriptase polymerase chain reaction (RT-PCR) and the protein by Western blotting. The same parameters were also determined in human livers. We detected IGFBP-3 mRNA in neonatal and adult as well as in fetal human heart tissues in both ventricles. Western blotting revealed the presence of IGFBP-3 in all the examined cardiac tissues. IGFBP-3 appeared to be more abundant in the heart than in the liver and in the failing hearts from patients with ischemic heart disease than in those with hypertrophic cardiomyopathy. Thus both normal and pathological human heart tissues express IGFBP-3 across lifespan and IGFBP-3 could play IGF-dependent and/or -independent actions at the myocardial level.
authors.affiliation: Department of Internal Medicine, University of Turin, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Granata
authors.forename: F
authors.initials: F
authors.lastname: Broglio
authors.forename: D
authors.initials: D
authors.lastname: Migliorino
authors.forename: S
authors.initials: S
authors.lastname: Cutrupi
authors.forename: G
authors.initials: G
authors.lastname: Baldanzi
authors.forename: M
authors.initials: M
authors.lastname: Sireno
authors.forename: A
authors.initials: A
authors.lastname: Fubini
authors.forename: A
authors.initials: A
authors.lastname: Grazian
authors.forename: E
authors.initials: E
authors.lastname: Ghigo
authors.forename: A
authors.initials: A
authors.lastname: Pucci
mesh_terms: D000328:Adult; D015153:Blotting, Western; D006332:Cardiomegaly / Q000378:metabolism*; D002311:Cardiomyopathy, Dilated / Q000378:metabolism*; D015870:Gene Expression; D006321:Heart / Q000196:embryology; D006801:Humans; D007231:Infant, Newborn; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000235:genetics*; D017202:Myocardial Ischemia / Q000378:metabolism*; D009206:Myocardium / Q000737:chemistry*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018972:Insulin-Like Growth Factor Binding Protein 3; D012333:RNA, Messenger