Cyanide-induced free radical production and lipid peroxidation in rat brain homogenate is reduced by aspirin.
The neuroprotective properties of aspirin were investigated using cyanide-induced neurotoxicity as model. Cyanide, a known neurotoxic agent significantly increased lipid peroxidation and superoxide anion levels in rat brain homogenate in a concentration-dependent manner (0.25-1.0 mM). When homogenate, containing 1.0 mM KCN was co-treated with aspirin (1.0 mM) there was a significant decrease in lipid peroxidation. Aspirin (0.5 mM and 1.0 mM) also significantly reduced KCN-induced superoxide anion generation. The results of the present report therefore indicate a neuroprotective role for aspirin.
Daya
S
S
Faculty of Pharmacy, Rhodes University, Grahamstown, South Africa. s.daya@ru.ac.za
Walker
R B
RB
Anoopkumar-Dukie
S
S
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001241:Aspirin / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects*; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D003486:Cyanides / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005609:Free Radicals / Q000378:metabolism*; D007202:Indicators and Reagents / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology; D009580:Nitroblue Tetrazolium / Q000494:pharmacology; D011190:Potassium Cyanide / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013481:Superoxides / Q000378:metabolism
D000894:Anti-Inflammatory Agents, Non-Steroidal; D003486:Cyanides; D005609:Free Radicals; D007202:Indicators and Reagents; D018696:Neuroprotective Agents; D013481:Superoxides; D009580:Nitroblue Tetrazolium; D011190:Potassium Cyanide; D001241:Aspirin