Sensitive detection of a plant virus by electrochemical enzyme-linked immunoassay.
The electrochemical enzyme-linked immunoassay increases the sensitivity of the detection of cucumber mosaic virus (CMV) by 5-fold compared with the spectrophotometric o-phenylenediamine (OPD) enzyme-linked immunosorbent assay (ELISA). The detection limit for the purified CMV is 1.0 ng/mL and the highest dilution ratio of the infected leaf sap is 1:5.0 x 10(4). The method is based on coupling the oxidation reaction of o-aminophenol (OAP)-H2O2 catalyzed by HRP-IgG conjugate with the electro-reduction of the enzymatic product. The enzymatic product 2-aminophenoxazine-3-one exhibits a sensitive second order derivative linear-sweep voltammetric response at the potential of -0.65 V (vs. Ag/AgCl) in pH 8.0 Britton-Robinson (B-R) buffer solution. So it can be applied to the detection of the plant virus with highly improved sensitivity.
Jiao
K
K
Department of Applied Chemistry, Qingdao Institute of Chemical Technology, Shandong, PR China. Jiaokui@public.qd.sd.cn
Sun
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W
Zhang
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SS
D017799:Cucumovirus / Q000302:isolation & purification*; D004563:Electrochemistry; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006735:Horseradish Peroxidase / Q000378:metabolism
D006735:Horseradish Peroxidase