Monoclonal antibodies to Hammondia hammondi allowing immunological differentiation from Toxoplasma gondii.
Five murine monoclonal antibodies (MAbs) were developed against purified sporozoites of Hammondia hammondi. Despite a large antigenic similarity between the 2 closely related coccidia, H. hammondi and Toxoplasma gondii, these MAbs only reacted with H. hammondi. Three MAbs, ID3, 3F2, and 4C9-7, recognized antigens of 38 kDa localized in rhoptries (1D3), in rhoptries and in oocyst and cyst walls (3F2), and in rhoptries and the apical region (4C9-7). Another MAb, 4C9-10, reacted with a 27-kDa antigen in dense granules of sporozoites and tachyzoites, and MAB 11B3 labeled an antigen of >94 kDa located in the pellicular membrane of the 3 stages of the parasite. These MAbs could be used for a rapid discrimination of the 2 coccidia in epidemiological studies or for diagnostic purposes in tissues.
Riahi
H
H
Service de Parasitologie-Mycologie, CHU Dupuyten, Limoges, France.
Leboutet
M J
MJ
Labrousse
F
F
Bouteille
B
B
Dardé
M L
ML
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D015153:Blotting, Western / Q000662:veterinary; D002371:Cat Diseases / Q000175:diagnosis* / Q000469:parasitology; D002415:Cats; D003048:Coccidiosis / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*; D003937:Diagnosis, Differential; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D006825:Hybridomas; D007150:Immunohistochemistry / Q000662:veterinary; D051379:Mice; D008807:Mice, Inbred BALB C; D016253:Microscopy, Immunoelectron / Q000662:veterinary; D021745:Sarcocystidae / Q000276:immunology / Q000302:isolation & purification*; D014122:Toxoplasma / Q000276:immunology / Q000302:isolation & purification*; D014124:Toxoplasmosis, Animal / Q000175:diagnosis
D000911:Antibodies, Monoclonal