Accuracy of t-PA, u-PA, PAI-1 and PAI-2 estimation in human bile by ELISA kits.
The aim of this study was to establish the accuracy of determination of fibrinolytic proteins in human bile using commercial kits.


METHODS
Gall bladder bile was obtained from patient undergoing laparoscopic cholecystectomy. The concentrations of tissue plasminogen activator (tPA), urokinase (uPA) and plasminogen activator inhibitor-1 (PAI-1) and inhibitor-2 (PAI-2) in bile were measured by enzyme linked immunoassay. Afterwards we conducted so called 'recovery test'.


RESULTS
Recovery percents of t-PA, u-PA, PAI-1, PAI 2 were very high i.e. 91.94, 94.07, 93.95, 91.2. We concluded that human fibrinolytic proteins in bile could be detected very accurately using commercial kits.
Kondera-Anasz
Z
Z
Department of Immunology and Serology, Silesian Medical University, Katowice, Poland.
Michalski
A
A
Gil
D
D
Gil
B
B
Starzewski
J
J
Gonciarz
Z
Z
D001646:Bile / Q000737:chemistry* / Q000201:enzymology; D017081:Cholecystectomy, Laparoscopic; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D017395:Plasminogen Activator Inhibitor 1 / Q000032:analysis*; D017396:Plasminogen Activator Inhibitor 2 / Q000032:analysis*; D011933:Reagent Kits, Diagnostic / Q000592:standards; D015203:Reproducibility of Results; D010959:Tissue Plasminogen Activator / Q000032:analysis*; D014568:Urokinase-Type Plasminogen Activator / Q000032:analysis*
D017395:Plasminogen Activator Inhibitor 1; D017396:Plasminogen Activator Inhibitor 2; D011933:Reagent Kits, Diagnostic; D010959:Tissue Plasminogen Activator; D014568:Urokinase-Type Plasminogen Activator