[PCR test systems for genetic identification of enterohemorrhagic Escherichia coli].
The analysis of modern data on the development of amplification test systems for the gene indication of enterohemorrhagic E. coli (EHEC) is presented. In this work the emphasis is laid on the importance of using specific primers whose nucleotide sequence is closely linked with genes controlling the key factors of EHEC pathogenicity; these factors include the determinants of the synthesis of adhesins and invasins (bfp, eae, tir), shiga-like toxins (stx1, stx2), enterohemolysin (ehx), serine protease (epsA) and specific LPS of O-antigen (rfb). The problem of using primers whose sequence is not linked with virulence genes, but which may also be used for the gene indication of E. coli O157:H7 (uid, fliC) is discussed.
Vorob'ev
A A
AA
Sechenov Medical Academy, Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia.
Bondarenko
V M
VM
Shabanova
N A
NA
Fialkina
S V
SV
D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D019453:Escherichia coli O157 / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods
D017931:DNA Primers; D004269:DNA, Bacterial
PTsR test-sistemy dlia genoindikatsii énterogemorragicheskikh ésherikhiĭ.