title: Phenotypic and functional differences between rat alveolar, pleural, and peritoneal macrophages.
abstract: Tissue macrophages (M phi) play a central and essential role in modulating the initiation and perpetuation of the inflammatory response. Phenotypical and functional differences among alveolar M phi (AM) and peritoneal M phi (PM) have been reported, but less is known about pleural M phi (PLM) and their ability and capacity to release biologically active substances. Therefore, the aim of this study was to determine the production of superoxide anion, nitric oxide (NO), and tumor necrosis factor alpha (TNF-alpha) by PLM in comparison to AM and PM in vitro. M phi from rats were isolated by lavage of the respective body compartment and characterized by evaluating the expression of the surface antigens MHC class II molecules, CD11b, and ED2-like antigen. Upon activation, AM produced significantly higher amounts of superoxide anion, NO, and TNF-alpha compared to PM and PLM. Taken together, the findings of this study demonstrate that rat PLM resemble PM more than AM in terms of production of key inflammatory mediators.
authors.affiliation: Institute for Surgical Research, Klinikum Grosshadern, University of Munich, Munich, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Dörger
authors.forename: S
authors.initials: S
authors.lastname: Münzing
authors.forename: A M
authors.initials: AM
authors.lastname: Allmeling
authors.forename: K
authors.initials: K
authors.lastname: Messmer
authors.forename: F
authors.initials: F
authors.lastname: Krombach
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002452:Cell Count; D008264:Macrophages / Q000166:cytology / Q000502:physiology*; D016676:Macrophages, Alveolar / Q000166:cytology / Q000502:physiology*; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000502:physiology; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D010641:Phenotype; D010994:Pleura / Q000166:cytology*; D051381:Rats; D011919:Rats, Inbred Strains; D013481:Superoxides / Q000378:metabolism; D007507:Therapeutic Irrigation; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000954:Antigens, Surface; D014409:Tumor Necrosis Factor-alpha; D013481:Superoxides; D009569:Nitric Oxide