Stability, toxicity and cytotoxicity of a cupric complex towards cultured CaCo-2 cells.
The human colon adenocarcinoma-derived cell line CaCo-2 was used as a model system to study the effects of copper (II), tetradentate N-alkyl ligand (H2L) and their complex. The stimulatory effect of the complex was obtained with lower concentrations from 10(-7) to 10(-17) mol.L-1, while inhibiting effects occur from 10(-4) to 10(-6) mol.L-1. According to this study, copper (II) and free ligand were toxic for cultured cells. Our data suggested that the complex, according to our toxicity assays in mice, could be used as an antimitotic agent.
Thanh
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Laboratoire de Physiologie (UPRES EA 2170), Facultés de Pharmacie et de Médecine, 16 boulevard Daviers, F-49100 Angers, France.
Djebbar-Sid
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Benali-Baïtich
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Pehu
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Khan
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MA
Bouet
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D000818:Animals; D018938:Caco-2 Cells / Q000187:drug effects*; D003300:Copper / Q000494:pharmacology* / Q000633:toxicity; D003497:Cyclic N-Oxides / Q000737:chemistry / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D008297:Male; D051379:Mice; D009942:Organometallic Compounds / Q000494:pharmacology / Q000633:toxicity
D003497:Cyclic N-Oxides; D009942:Organometallic Compounds; C420460:diaqua(N,N'-bis(2-hydroxyacetophenyl)-1,2-diaminoethane)copper(II); C420482:tempicol 3; D003300:Copper; C029892:cupric chloride