title: DNA cytofluorometric analysis of benign and malignant nerve sheath tumors.
abstract: The present study was undertaken to examine the ploidy patterns of benign and malignant nerve sheath tumors using PI-DNA cytofluorometry and to clarify the relationship between the ploidy patterns and pathological findings for these lesions. The benign nerve sheath tumors (schwannomas and neurofibromas) were found to be dipolid or to be euploid-polyploidization, with a few DNA synthetic cells. The number of polyploid cells increased with increase in the frequency of cells with large and atypical nuclei in histology, regardless of the natures of cellular arrangements. Malignant schwannomas were composed of many polyploid and aneuploid cells with DNA synthetic cells, indicating their active cell proliferation. Thus, benign nerve sheath tumors exhibited low-proliferative ploidy patterns that were clearly different from those of malignant nerve sheath tumors. As Takeshita has already stated, it is difficult to ascertain why some of the neurogenic tumors show euploid-polyploidization (19). Further investigations will be needed in this direction. Finally, it is clear from the results of this study that the cytofluorometric analysis mentioned above is useful for assessing malignancy and that it may be useful for predicting prognosis of nerve sheath tumors.
authors.affiliation: Department of Orthopaedic Surgery, Seika Hospital, Kyoto 619-0241, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Tsuji
authors.forename: K
authors.initials: K
authors.lastname: Kusuzaki
authors.forename: A
authors.initials: A
authors.lastname: Kuzuhara
authors.forename: H
authors.initials: H
authors.lastname: Murata
authors.forename: H
authors.initials: H
authors.lastname: Takeshita
authors.forename: M
authors.initials: M
authors.lastname: Hirata
authors.forename: S
authors.initials: S
authors.lastname: Hashiguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Hirasawa
authors.forename: T
authors.initials: T
authors.lastname: Ashihara
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000782:Aneuploidy; D002455:Cell Division; D002648:Child; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D018317:Nerve Sheath Neoplasms / Q000235:genetics* / Q000473:pathology; D009442:Neurilemmoma / Q000235:genetics / Q000473:pathology; D009455:Neurofibroma / Q000235:genetics / Q000473:pathology; D017253:Neurofibromatoses / Q000235:genetics / Q000473:pathology; D011003:Ploidies; D011123:Polyploidy
chemical_list: D004273:DNA, Neoplasm