Selective reaction to a flipping cytidine of the duplex DNA mediated by triple helix formation.
A new nucleoside derivative (2) with a butyl spacer between the sugar part and the 2-amino-6-vinylpurine motif has been synthesized. The triplex-forming oligodeoxynucleotide incorporating 2 has achieved strand- and cytidine-selective cross-linking reaction to the G-C target site mediated by triple helix formation. It has been suggested that 2 reacts with a flipping cytidine at the target site.
Nagatsugi
F
F
Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Usui
D
D
Kawasaki
T
T
Maeda
M
M
Sasaki
S
S
D020137:Base Pair Mismatch; D020029:Base Pairing; D003432:Cross-Linking Reagents / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D003562:Cytidine / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D004586:Electrophoresis; D009690:Nucleic Acid Conformation; D009705:Nucleosides / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D013329:Structure-Activity Relationship; D013696:Temperature
D003432:Cross-Linking Reagents; D009705:Nucleosides; D009838:Oligodeoxyribonucleotides; D003562:Cytidine; D004247:DNA