Significance of detection of Porphyromonas gingivalis, Bacteroides forsythus and Treponema denticola in periodontal pockets.
The relationship between the detection of Porphyromonas gingivalis, Bacteroides forsythus and Treponema denticola in subgingival plaque samples of periodontal pockets and periodontal status was evaluated using the polymerase chain reaction (PCR). A total of 165 sites in 60 periodontitis patients were examined, and the relationships between the detection of each of the three bacterial species and the pocket depth and bleeding on probing (BOP) were analyzed. The detection ratios of P. gingivalis, B. forsythus, and T. denticola in samples from adult periodontitis lesions were 75.5%, 69.8%, and 72.6%, respectively. It was found that all sites where all three microorganisms were detected were BOP positive and had greater pocket depths than those where only one or two species were found. The detection rate of B. forsythus and T. denticola decreased with age in the sites in which PD was less than 4 mm. The present study indicates that detection of a mixed infection by P. gingivalis, B. forsythus, and T. denticola strongly correlated with adult periodontitis.
Kasuga
Y
Y
Department of Microbiology, Tokyo Dental College, 1-2-2 Masago, Mihama-ku, Chiba 261-8502, Japan.
Ishihara
K
K
Okuda
K
K
D000328:Adult; D000367:Age Factors; D001439:Bacteroides / Q000145:classification* / Q000235:genetics; D016009:Chi-Square Distribution; D003773:Dental Plaque / Q000382:microbiology; D005884:Gingival Hemorrhage / Q000145:classification / Q000382:microbiology; D006801:Humans; D008875:Middle Aged; D010514:Periodontal Pocket / Q000145:classification / Q000382:microbiology*; D010518:Periodontitis / Q000382:microbiology; D016133:Polymerase Chain Reaction; D016966:Porphyromonas gingivalis / Q000145:classification* / Q000235:genetics; D018709:Statistics, Nonparametric; D014208:Treponema / Q000145:classification* / Q000235:genetics