[Reverse transcriptase polymerase chain reaction and immunocytochemistry of bone marrow aspiration specimen and peripheral blood for detection of microdisseminated prostatic carcinoma. A comparative analysis].
Reverse transcriptase-polymerase chain reaction (RT-PCR) assay for prostate-specific antigen and immunocytochemistry for cytokeratin-18 (CK-18) are tests for the detection of microdisseminated carcinoma of the prostate. Bone marrow aspirates and peripheral venous blood from 50 patients with clinically organ-confined prostate cancer were examined. The rate of positive results was independent of the pT stage, serum PSA, and previous antiandrogen treatment. RT-PCR and immunocytochemistry have to be tested under standardized conditions in prospective trials, and the results have to be compared to the serum PSA follow-up.
Borchers
H
H
Urologische Klinik, Universitätsklinikum, RWTH Aachen.
Wolff
J M
JM
Brehmer
B
B
Beniers
A J
AJ
Jakse
G
G
D000368:Aged; D001707:Biopsy, Needle; D001853:Bone Marrow / Q000473:pathology; D019046:Bone Marrow Neoplasms / Q000473:pathology / Q000556:secondary*; D006801:Humans; D007633:Keratins / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D009360:Neoplastic Cells, Circulating; D011237:Predictive Value of Tests; D011379:Prognosis; D011471:Prostatic Neoplasms / Q000473:pathology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D007633:Keratins
Reverse Transkriptase-Polymerase-Kettenreaktion und Immunozytochemie an Knochenmarkaspiraten und peripherem Blut zum Nachweis mikrodisseminierter Prostatakarzinome. Eine vergleichende Analyse.