title: The localization of persistent foot and mouth disease virus in the epithelial cells of the soft palate and pharynx.
abstract: After contact with foot and mouth disease virus (FMDV), cattle may become persistently infected, regardless of their pre-existing immune status or whether they develop clinical disease. The cellular sites of FMDV persistence have not previously been determined. The use of in-situ hybridization in combination with tyramide signal amplification (TSA) provided the first direct evidence that FMDV RNA is localized within the epithelial cells of the soft palate and pharynx during persistent infection, indicating that these cells remain persistently infected after contact with FMDV.
authors.affiliation: Pirbright Laboratory, Institute for Animal Health, World Reference Laboratory for Foot and Mouth Disease, Ash Road, Pirbright, Surrey, GU24 0NF, UK.
authors.forename: Z D
authors.initials: ZD
authors.lastname: Zhang
authors.forename: R P
authors.initials: RP
authors.lastname: Kitching
mesh_terms: D000818:Animals; D005537:Aphthovirus / Q000235:genetics / Q000302:isolation & purification*; D001826:Body Fluids / Q000821:virology; D002417:Cattle; D002418:Cattle Diseases / Q000473:pathology / Q000821:virology*; D004847:Epithelial Cells / Q000473:pathology / Q000821:virology*; D005536:Foot-and-Mouth Disease / Q000473:pathology / Q000821:virology*; D017403:In Situ Hybridization / Q000662:veterinary; D015345:Oligonucleotide Probes / Q000737:chemistry; D010160:Palate, Soft / Q000473:pathology / Q000821:virology*; D010614:Pharynx / Q000473:pathology / Q000821:virology*; D012367:RNA, Viral / Q000032:analysis
chemical_list: D015345:Oligonucleotide Probes; D012367:RNA, Viral