ELF magnetic fields initiate protein tyrosine phosphorylation of the T cell receptor complex.
The human T cell line Jurkat registers a sinusoidal extremely low frequency (ELF), 0.10 mT magnetic fields (MFs) at the level of the plasma membrane. In this study, the protein tyrosine phosphorylation (PY) of two membrane-associated proteins in Jurkat cells were examined following a short-term MFs exposure, the zeta chains and the Src kinases p56lck. These proteins are interesting to study since the earliest biochemical event upon T cell receptor (TcR) activation is PY of the zeta chains. These signalling chains in the TcR complex was assessed using Western blotting and the activation of the p56lck kinase was analysed by in vitro kinase assay. The MFs exposure of Jurkat for 5 min activated p56lck and resulted in PY of zeta. These findings are in line with earlier reports on how MFs exposure affects signal transduction in Jurkat.
Lindström
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E
National Institute for Working Life, Umeå, Sweden. Ewa.Lindstrom@mtc.ki.se
Still
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M
Mattsson
M O
MO
Mild
K H
KH
Luben
R A
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D015153:Blotting, Western; D006801:Humans; D019169:Jurkat Cells / Q000528:radiation effects; D007700:Kinetics; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck) / Q000378:metabolism / Q000528:radiation effects*; D008280:Magnetics; D008565:Membrane Proteins / Q000528:radiation effects; D010766:Phosphorylation / Q000528:radiation effects; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism / Q000528:radiation effects*; D015398:Signal Transduction / Q000528:radiation effects
D008565:Membrane Proteins; D011948:Receptors, Antigen, T-Cell; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck)