title: A comparison between human, crustacean and bivalve isolates of Microsporidia.
abstract: Identification of Microsporidia detected in diarrhoeic stool of human and from press preparation of crabs, and bivalves muscles was done by staining with Giemsa, modified trichrome and H & E stains respectively. Positive samples were isolated & used for infection of 3 groups of mice viz group A human isolate, group B crab & group C bivalve isolates. Parasitological and histopathological studies using light and transmission electron microscopy (TEM) were done for samples from each group. The results clarified that human, crab & bivalve Microsporidia isolates showed common features. They were found in the supranuclear region of enterocytes. The stages observed were meronts, sporonts and free spores. Each spore was 1-2 um in length containing a single nucleus, a vacuole and a polar filament. Further genetical and biochemical studies will be undertaken to confirm the similarity or differences between these isolates.
authors.affiliation: Department Parasitology, Faculty of Medicine, Alexandria University, Alexandria, Egypt.
authors.forename: H N
authors.initials: HN
authors.lastname: Awadalla
authors.forename: A M
authors.initials: AM
authors.lastname: Khalifa
authors.forename: H A
authors.initials: HA
authors.lastname: Sadaka
authors.forename: S M
authors.initials: SM
authors.lastname: Helal
mesh_terms: D000818:Animals; D003445:Crustacea / Q000469:parasitology*; D003967:Diarrhea / Q000469:parasitology; D005243:Feces / Q000469:parasitology; D006801:Humans; D051379:Mice; D008854:Microscopy, Electron; D016814:Microsporidia / Q000302:isolation & purification* / Q000648:ultrastructure