Development and validation of a high-performance liquid chromatographic method for the determination of clomazone residues in surface water.
A method is described for the determination of clomazone residues in surface water by high-performance liquid chromatography with UV detection. The method involves solid-phase extraction with C18 extraction tubes. Clomazone was separated on a C18 column with a mobile phase of methanol-water (65:35, v/v) at pH 4.0 and a flow-rate of 1.0 ml/min. After optimization of the extraction and separation conditions, the method was validated. The method developed can be used for determination of clomazone in surface water, at the limit of 0.1 mcirog/l set by the European Union drinking water directive, with a 400-fold preconcentration.
Zanella
R
R
Departamento de Química, Universidade Federal de Santa Maria, RS, Brazil.
Primel
E G
EG
Gonçalves
F F
FF
Martins
A F
AF
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007555:Isoxazoles / Q000032:analysis*; D023303:Oxazolidinones / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D014874:Water Pollutants, Chemical / Q000032:analysis*
D007555:Isoxazoles; D023303:Oxazolidinones; D010573:Pesticide Residues; D014874:Water Pollutants, Chemical; C095255:clomazone