Fast temperature programming in routine analysis of multiple pesticide residues in food matrices.
Flash gas chromatographic (GC) analysis of 15 organophosphorus pesticides commonly occurring in food crops was performed using the Thermedics Detection EZ Flash upgrade kit installed in the oven of a HP 5890 Series II Plus gas chromatograph. The temperature program and splitless time period were the main parameters to be optimized. In the first set of experiments wheat matrix-matched standards were analyzed both by: (i) the flash GC technique (resistive heating of a 5 m capillary column), and (ii) the conventional GC technique (moderate oven temperature programming of a 30 m capillary column). Using the flash GC technique, the analysis time was reduced by a factor of more than 10 compared to the conventional GC technique. Dramatically improved detectability of analytes was achieved due to much narrower peak widths. The flash GC technique was compared with another approach to faster GC analysis employing a 5 m column and fast temperature programming with a conventional GC oven. In comparison with this alternative, in the case of flash GC significantly better retention time repeatability was observed. The other superiority of resistive heating is very rapid cooling down (i.e., equilibration to the initial conditions) which contributes to the increased sample throughput.
Mastovská
K
K
Institute of Chemical Technology, Department of Food Chemistry and Analysis, Technická, Prague, Czech Republic.
Hajslová
J
J
Godula
M
M
Krivánková
J
J
Kocourek
V
V
D002849:Chromatography, Gas / Q000379:methods*; D018556:Crops, Agricultural / Q000737:chemistry*; D005506:Food Contamination / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*; D012015:Reference Standards; D013696:Temperature
D010573:Pesticide Residues