Macrophage-like cell line (HS-P) from a rat histiocytic sarcoma.
With future exploration of macrophage properties in mind, we established a novel cell line (HS-P) from a transplantable histiocytic sarcoma, derived originally from a tumour in an aged F344 rat. HS-P was subjected to 70 serial passages, in which the mean doubling time was 15.7 h. The cells, which were round, oval or polygonal in shape, were arranged in a compact sheet. They reacted to varying degrees for lysosomal enzymes (acid phosphatase and non-specific esterase) and with the following antibodies: ED1/ED2 (rat macrophage/histiocyte-specific), OX6 (rat MHC class II-specific), lysozyme antibody and alpha1-antichymotrypsin antibody. Electron microscopically, HS-P cells showed lysosomes and prominent cell projections. These findings indicated that the cultured cells were macrophage-like. Syngeneic rats inoculated subcutaneously or intraperitoneally with HS-P cells invariably developed sarcomatous tumours consisting of monomorphic mononuclear cells, which exhibited cytochemical properties similar to those of cultured HS-P cells. Bioassay and reverse transcription-polymerase chain reaction methods revealed that tumour necrosis factor-alpha increased on addition of lipopolysaccharide (LPS), indicating that HS-P cells remained LPS-responsive. HS-P cells may prove to be a useful tool for in-vitro studies of macrophage function.
Yamate
J
J
Department of Veterinary Pathology, College of Agriculture, Osaka Prefecture University, Gakuencho 1-1, Sakai 599-8531, Osaka, Japan.
Maeda
M
M
Tsukamoto
Y
Y
Benn
S J
SJ
Laithwaite
J E
JE
Allan
A
A
Kannan
Y
Y
Ide
M
M
Kuwamura
M
M
Kotani
T
T
Sakuma
S
S
LaMarre
J
J
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D002452:Cell Count / Q000662:veterinary; D005260:Female; D018219:Histiocytoma, Benign Fibrous / Q000201:enzymology / Q000276:immunology / Q000473:pathology*; D008113:Liver Neoplasms / Q000201:enzymology / Q000276:immunology / Q000473:pathology*; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology; D008264:Macrophages / Q000201:enzymology / Q000276:immunology / Q000473:pathology*; D008297:Male; D009113:Muramidase / Q000378:metabolism; D009368:Neoplasm Transplantation; D015388:Organelles / Q000648:ultrastructure; D051381:Rats; D011916:Rats, Inbred F344; D012513:Sarcoma, Experimental / Q000201:enzymology / Q000276:immunology / Q000473:pathology*; D013047:Specific Pathogen-Free Organisms; D014407:Tumor Cells, Cultured / Q000201:enzymology / Q000276:immunology / Q000473:pathology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D000951:Antigens, Neoplasm; D014409:Tumor Necrosis Factor-alpha; C026656:oxindoleamine-62 lysozyme; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; D000135:Acid Phosphatase; D009113:Muramidase