title: The mechanism of DNA repair by uracil-DNA glycosylase: studies using nucleotide analogues.
abstract: 2',4'-Dideoxy-4'-methyleneuridine incorporated into oligodeoxynucleotides forms regular B-DNA duplexes as shown by Tm and CD measurements. Such oligomers are not cleaved by the DNA repair enzyme, UDG, which cleaves the glycosylic bond in dU but not in dT nor in dC nucleosides in single stranded and double stranded DNA. Differential binding of oligomers containing carbadU, 4'-thiodU, and dU residues to wild type and mutant UDG proteins identify an essential role for the furanose 4'-oxygen in recognition and cleavage of dU residues in DNA.
authors.affiliation: Krebs Institute, Department of Chemistry, Sheffield University, UK.
authors.forename: A
authors.initials: A
authors.lastname: Rösler
authors.forename: G
authors.initials: G
authors.lastname: Panayotou
authors.forename: D P
authors.initials: DP
authors.lastname: Hornby
authors.forename: T
authors.initials: T
authors.lastname: Barlow
authors.forename: T
authors.initials: T
authors.lastname: Brown
authors.forename: L H
authors.initials: LH
authors.lastname: Pearl
authors.forename: R
authors.initials: R
authors.lastname: Savva
authors.forename: G M
authors.initials: GM
authors.lastname: Blackburn
mesh_terms: D001483:Base Sequence; D045647:DNA Glycosylases; D004260:DNA Repair; D018259:Herpesvirus 1, Human / Q000201:enzymology; D009682:Magnetic Resonance Spectroscopy; D009699:N-Glycosyl Hydrolases / Q000378:metabolism*; D009711:Nucleotides / Q000378:metabolism*; D020349:Surface Plasmon Resonance; D051981:Uracil-DNA Glycosidase
chemical_list: D009711:Nucleotides; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D051981:Uracil-DNA Glycosidase