Effect of cysteamine during in vitro maturation on buffalo embryo development.
The purpose of this study was to evaluate whether the addition of cysteamine during in vitro maturation (IVM) of buffalo oocytes enhances embryo development. Cumulus-oocyte complexes (COC) from slaughterhouse ovaries were matured in vitro in TCM 199 supplemented with 10% fetal calf serum (FCS), 0.5 microg mL(-1) FSH, 5 microg mL(-1) LH, 1 microg mL(-1) 17 beta estradiol and 0 (control), 50, 100 or 200 micromol L(-1) of cysteamine for 24 hours. The matured oocytes then were fertilized and cultured for 7 days. No beneficial effect on maturation and cleavage rate was related to the addition of cysteamine. However, the percentage of embryos that developed to compact morula and blastocyst stage was significantly higher (P < or = 0.01) for oocytes matured in medium containing 50 micromol L(-1) of cysteamine than it was for oocytes matured with 0, 100 and 200 micromol L(-1) cysteamine (22.6% vs 14.9%, 15.7% and 13% respectively); moreover, the addition of 50 micromol L(-1) of cysteamine during IVM significantly (P < or = 0.01) increased the proportion of transferable quality (Grades 1 and 2) embryos (19.3% vs 11.3%, 11.6% and 11.2% respectively). The present study showed that adding a thiol compound (such as cysteamine) to the IVM medium improves buffalo in vitro embryo production (IVEP) efficiency, which so far has been unsatisfactory.
Gasparrini
B
B
Department of Scienze Zootecniche ed Ispezione degli Alimenti, Faculty of Veterinary Medicine, Napoli, Italy. bgasparr@unina.it
Neglia
G
G
Palo
R D
RD
Campanile
G
G
Zicarelli
L
L
D000818:Animals; D001755:Blastocyst / Q000502:physiology; D002020:Buffaloes / Q000196:embryology*; D046508:Culture Techniques; D003543:Cysteamine / Q000494:pharmacology*; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary*; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D007986:Luteinizing Hormone / Q000494:pharmacology; D009028:Morula / Q000502:physiology
D004958:Estradiol; D003543:Cysteamine; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone