Hibernated human fetal striatal tissue: successful transplantation in a rat model of Huntington's disease.
The use of fresh human fetal tissue in neural transplantation presents considerable logistical difficulties and limits the clinical applicability of this promising therapy. This study compared the survival of human fetal striatal tissue that had been stored for 24 h in a defined hibernation medium with that of fresh human fetal striatal tissue following xenotransplantation in a rat model of Huntington's disease (HD). Six to 7 weeks postgrafting, there was no significant difference between fresh and hibernated grafts in volume or in various striatal phenotypic markers, although there was a trend towards decreased graft volume. We conclude that short-term hibernation of this tissue is without significant adverse effects on the survival of grafted human fetal striatal tissue. This has important implications for the practical implementation of clinical neural transplant programs in HD.
Hurelbrink
C B
CB
Cambridge Centre for Brain Repair, University of Cambridge, UK. cbh20@cam.ac.uk
Armstrong
R J
RJ
Barker
R A
RA
Dunnett
S B
SB
Rosser
A E
AE
D000110:Acetylcholinesterase / Q000032:analysis; D000818:Animals; D016380:Brain Tissue Transplantation / Q000379:methods*; D002478:Cells, Cultured; D003342:Corpus Striatum / Q000637:transplantation*; D004195:Disease Models, Animal; D051937:Dopamine and cAMP-Regulated Phosphoprotein 32; D005260:Female; D016332:Fetal Tissue Transplantation / Q000379:methods*; D006085:Graft Survival; D006801:Humans; D006816:Huntington Disease / Q000601:surgery*; D009412:Nerve Fibers / Q000737:chemistry / Q000201:enzymology; D009419:Nerve Tissue Proteins; D009474:Neurons / Q000637:transplantation*; D010750:Phosphoproteins / Q000032:analysis; D051381:Rats; D011916:Rats, Inbred F344; D014021:Tissue Preservation / Q000379:methods*; D014183:Transplantation, Heterologous; D016875:tau Proteins / Q000032:analysis
D051937:Dopamine and cAMP-Regulated Phosphoprotein 32; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; D016875:tau Proteins; D000110:Acetylcholinesterase