Determination of underivatized amino acids by high-performance liquid chromatography and electrochemical detection at an amino acid oxidase immobilized CuPtCI6 modified electrode.
An amperometric sensor for amino acids based on the immobilization of amino acid oxidase on the surface of a CuPtCl(6)/GC is described. The amperometric current is due to the oxidation of H2O2 liberated during the enzyme reaction on the surface of the enzyme electrode. The electrode response characteristics as well as kinetic parameters have been evaluated. The enzyme electrode was characterized as an electrochemical biosensor, which was used as detector in high performance liquid chromatography (HPLC) for the determination of a mixture of amino acids with satisfactory results.
Pei
J
J
Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon.
Li
X
X
D000594:Amino Acid Oxidoreductases / Q000737:chemistry*; D000596:Amino Acids / Q000032:analysis*; D002138:Calibration; D002712:Chlorides / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D017671:Platinum Compounds / Q000737:chemistry*; D013696:Temperature
D000596:Amino Acids; D002712:Chlorides; D004800:Enzymes, Immobilized; D017671:Platinum Compounds; C422063:copper platinum chloride; D000594:Amino Acid Oxidoreductases