Voltammetric determination of N,N'-dinitrosopiperazine in simulated gastric juice.
A voltammetric method has been developed for the determination of N,N'-dinitrosopiperazine (DNPZ) in simulated gastric juice. The method is based on measuring the differential pulse polarographic peak produced in pH 3 Britton Robinson buffer. A well defined, diffusion-controlled cathodic wave was obtained at -0.77 V versus Ag/AgCl over the range 0.4-24 microg/ml with minimum detectability (S/N = 2) of 0.072 microg/ml (5 x 10(-7) M). The proposed method was successfully applied to study the possible in vivo production of the nitroso-derivatives of piperazine under the standard nitrosation reaction conditions recommended by WHO. The method has some distinct advantages over the reported GC methods.
Belal
F
F
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, King Saud University, Riyadh, Saudi Arabia. ffbelal@ksu.edu.sa
Walash
M I
MI
Ibrahim
F
F
Hefnawy
M
M
Eid
M
M
D004583:Electrons; D005750:Gastric Juice / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D009602:Nitrosamines / Q000032:analysis*; D011048:Polarography; D012015:Reference Standards; D012996:Solutions
D007202:Indicators and Reagents; D009602:Nitrosamines; D012996:Solutions; C026156:N,N'-dinitrosopiperazine