Differentiation of Ly49s-positive or -negative natural killer cells is inhibited by anti-H-2b monoclonal antibodies acting at the level of bone marrow progenitors from B6 mice.
To investigate the role of MHC class I on in vitro differentiation of natural killer (NK) cells, a CD44low/-CD2-classlow population was isolated from mouse bone marrow. This population, which lacked expression of NK-1.1, Ly49A, Ly49C/I, and Ly49G, generated populations of NK-1.1+ NK cells expressing Ly49A, Ly49C/I, or Ly49G when cocultured for 13 days with syngeneic supportive stromal cells in the presence of interleukin 2. Ly49A and Ly49C/I were absent on the progeny of progenitors tested after 7 days of culture but were expressed as a late event together with low-level expression of NK-1.1, from day 8 of culture. The addition of anti-H-2b monoclonal antibody to cultures at day 0 inhibited proliferation of progenitors supported by either syngeneic, allogeneic, or H-2b-deficient stromal cells, thus suggesting that the effect was not exerted on stromal cells. Additional analyses demonstrated that class Ilow progenitors generated class I+ cells on which the anti-H-2b monoclonal antibody exerted its inhibitory effect.
Delfino
D V
DV
Department of Clinical and Experimental Medicine, Section of Pharmacology, University of Perugia, Italy. farmaco@unipg.it
Salcedo
M
M
Marco
B D
BD
Ayroldi
E
E
Nocentini
G
G
Bruscoli
S
S
Brunetti
L
L
Ljunggren
H G
HG
Riccardi
C
C
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D000950:Antigens, Ly; D001854:Bone Marrow Cells / Q000378:metabolism*; D002454:Cell Differentiation; D002478:Cells, Cultured; D018920:Coculture Techniques; D005434:Flow Cytometry; D005805:Genes, MHC Class I; D007376:Interleukin-2 / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000378:metabolism*; D037181:Lectins, C-Type; D008280:Magnetics; D008562:Membrane Glycoproteins / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D055650:NK Cell Lectin-Like Receptor Subfamily A; D010641:Phenotype; D055630:Receptors, NK Cell Lectin-Like; D013997:Time Factors
D000911:Antibodies, Monoclonal; D000950:Antigens, Ly; D007376:Interleukin-2; C527088:Klra1 protein, mouse; C527091:Klra3 protein, mouse; C527095:Klra7 protein, mouse; D037181:Lectins, C-Type; D008562:Membrane Glycoproteins; D055650:NK Cell Lectin-Like Receptor Subfamily A; D055630:Receptors, NK Cell Lectin-Like