[Analysis of phenylethanoid glycosides in the extract of herba Cistanchis by LC/ESI-MS/MS].
OBJECTIVE
To analyze the phenylethanoid glycosides in Cistanche deserticola Y. D. Ma and its alternatives.


METHODS
An HPLC/MS/MS method has been developed for the analysis of seven kinds of phenylethanoid glycosides in Cistanche deserticola Y. D. Ma, C. salsa (C. A. Mey) G. Beck and C. tubulosa (Schenk) R. Wight. The [M - H]- ions were observed for five standards and Cistanche extracts. The glycosidic linkages, the core, and the attached sugar (s) of the phenylethanoid glycosides can be determined from the collision-induced dissociation spectra of the molecular.


RESULTS
Seven kinds of phenylethanoid glycosides (echinacoside, acteoside, cisacteoside, isoacteoside, 2'-acetylacteoside, cistanoside A, osmanthuside B) in Cistanche deserticola Y. D. Ma, six kinds (echinacoside, acteoside, cisacteoside, isoacteoside, 2'-acetylacteoside and cistanoside A) in C. salsa (C. A. Mey) G. Beck and five kinds (echinacoside, acteoside, cisacteoside, isoacteoside and 2'-acetylacteoside) in C. tubulosa (Schenk) R. Wight were detected.


CONCLUSIONS
The difference of the relative distribution of these phenylethanoid glycosides in each extract was found out. Phenylethanoid glycosides are the specific constituents in Cistanchis, which can be used to distinguish different species in Genus Cistanchis.
Wang
Y M
YM
Department of Chemistry, Tsinghua University, Beijing 100084, China.
Zhang
S J
SJ
Luo
G A
GA
Hu
Y N
YN
Hu
J P
JP
Liu
L
L
Zhu
Y
Y
Wang
H J
HJ
D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D006027:Glycosides / Q000032:analysis* / Q000737:chemistry; D010636:Phenols / Q000032:analysis* / Q000737:chemistry
D004365:Drugs, Chinese Herbal; D006027:Glycosides; D010636:Phenols