Angelica sinensis modulates migration and proliferation of gastric epithelial cells.
A crude extract from Angelica sinensis (ASCE), which mainly consists of polysaccharides, prevents ethanol- or indomethacin-induced gastric mucosal damage and promotes ulcer healing. The aim of this study was to test the hypothesis that ASCE has a direct stimulating effect on gastric epithelial cells for wound healing. We found that ASCE significantly promoted the migration of epithelial cells over an artificial wound on the surface of an RGM-1 monolayer. The extract also stimulated DNA synthesis in a dose-dependent manner and concomitantly increased EGF mRNA expression. Co-incubation of ASCE with anti-EGF antibody reduced the speed of migration and the DNA synthesis, which however were still higher than the control without ASCE. These results strongly suggest that ASCE has a direct wound healing effect on gastric mucosa, and this is acting partially through an EGF-mediated pathway.
Ye
Y N
YN
Department of Pharmacology, Zhejiang University, Hanzhou, China.
Koo
M W
MW
Li
Y
Y
Matsui
H
H
Cho
C H
CH
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D018929:Cell Culture Techniques; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004815:Epidermal Growth Factor / Q000276:immunology; D004847:Epithelial Cells / Q000187:drug effects / Q000473:pathology; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D005765:Gastrointestinal Agents / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011134:Polysaccharides / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis; D013276:Stomach Ulcer / Q000188:drug therapy / Q000473:pathology; D013936:Thymidine / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects*
D000906:Antibodies; D005765:Gastrointestinal Agents; D010936:Plant Extracts; D011134:Polysaccharides; D012333:RNA, Messenger; D004815:Epidermal Growth Factor; D013936:Thymidine