[Antifungal effect of three natural products on the genetic substance of Saccharomyces cerevisiae GL7 and Prototheca wickerhamii].
OBJECTIVE
To investigate the antifungal effect of three natural products on the genetic substance of Saccharomyces cerevisiae GL7 and Prototheca wickerhamii.


METHODS
The normal and treated cells were observed by confocal laser scanning microscope (CLSM) and image analysis to quantitatively described the cell morphology, area, DNA and RNA content.


RESULTS
The morphology, area, DNA and RNA contents were changed greatly in the treated cells.


CONCLUSIONS
Solasodine, 4'-hydroxy-3, 5-dimethoxystilbene and dictamnine directly or indirectly interfered the synthesis and function of genetic substance in S. cerevisiae and P. wickerhamii.
Wang
L D
LD
School of Pharmaceutical Sciences, Peking University, Beijing 100083, China.
Guo
D A
DA
Yuan
L
L
He
Q H
QH
Hu
Y Q
YQ
Tu
P F
PF
Zheng
J H
JH
D000470:Alkaloids / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology*; D004271:DNA, Fungal / Q000187:drug effects*; D011525:Prototheca / Q000187:drug effects* / Q000235:genetics; D011804:Quinolines; D012331:RNA, Fungal / Q000187:drug effects*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics; D012991:Solanaceous Alkaloids / Q000494:pharmacology
D000470:Alkaloids; D000935:Antifungal Agents; D004271:DNA, Fungal; D011804:Quinolines; D012331:RNA, Fungal; D012991:Solanaceous Alkaloids; C026398:dictamnine; C012037:solasodine