Sister chromatid exchanges and chromosome aberrations in V79 cells induced by aflatoxin B1, B2, G1 and G2 with or without metabolic activation.
Cells from Chinese hamster cell line V79 were pulse treated for one hour with aflatoxin (AF) B1, B2, G1 and G2 at various doses with or without the metabolic activation system-S9 mix. With metabolic activation, all these toxins caused significant increases of frequencies of sister chromatid exchanges (SCE) in a dose-dependent manner. Based on the rate of SCE induction, the relative order of potency was established as AFB1 > G1 > G2 > B2. Without metabolic activation, all four toxins had no or very little effect over the same dose ranges used for SCE induction with activation. Induction of chromosome aberrations in V79 cells after a one hour pulse treatment with AFB1 and G1 plus S9 mix was also studied. A dose and time dependent increase of aberrations was observed for both compounds. AFB1 again is a more potent aberration inducer than AFG1.
Batt
T R
TR
Department of Experimental Biology, Roswell Park Memorial Institute, Buffalo, NY, USA.
Hsueh
J L
JL
Chen
H H
HH
Huang
C C
CC
D000348:Aflatoxins / Q000493:pharmacokinetics* / Q000633:toxicity*; D000818:Animals; D001711:Biotransformation; D001973:Bromodeoxyuridine / Q000378:metabolism; D002460:Cell Line; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D004305:Dose-Response Relationship, Drug; D008677:Metaphase / Q000187:drug effects; D008940:Mitotic Index; D012854:Sister Chromatid Exchange
D000348:Aflatoxins; D001973:Bromodeoxyuridine