Effects of a membrane-metallopeptidase blocking agent thiorphan in long-term cultures of human bone marrow.
Thiorphan [(DL-3-mercapto-2-benzylpropanoyl)-glycine], a drug blocking the activity of membrane metalloendopeptidase EC 3.4.24.11 (CD10, CALLA), was added to long-term cultures of human bone marrow. Progression of the cultures was assessed by cell counts, cytology and clonogenic (GM-CFU) ability of the non-adherent cells in the supernatant and by morphology of the adherent stromal layer. A stimulatory effect on hematopoiesis was noted.
Stanović Janda
S
S
Ruder Bosković Institute, Department of Molecular Medicine, Zagreb, Croatia. stanovic@rudjer.irb.hr
Boranić
M
M
Sucić
M
M
Petrovecki
M
M
Golubić-Cepulić
B
B
Aurer
I
I
Labar
B
B
D001854:Bone Marrow Cells / Q000187:drug effects* / Q000473:pathology*; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D015260:Neprilysin / Q000037:antagonists & inhibitors*; D011480:Protease Inhibitors / Q000494:pharmacology*; D015244:Thiorphan / Q000494:pharmacology*
D011480:Protease Inhibitors; D015244:Thiorphan; D015260:Neprilysin