Splice isoforms of transcription factor Elf-1 affecting its regulatory function in transcription-molecular cloning of rat Elf-1.
To elucidate the role of Elf-1 in Fc epsilonRI alpha chain expression, rat Elf-1 cDNAs were isolated and characterized. The rat Elf-1 cDNA of 2744 bp contained an open reading frame of 1848 bp. In addition to the full length rat Elf-1 cDNA (named type 1), two splice isoforms were isolated. One of the two isoforms lacked the amino acid residues from 85th to 120th (type 2), and the other from 85th to 175th (type 3). Similar isoforms were also observed in human tissue. Overexpression of rat Elf-1 (type 1) using a transient coexpression system inhibited of the alpha chain promoter activity. The inhibition activity was different between the isoforms; the inhibition activity of type 2 was lower than that of type 1, and type 3 did not have an inhibitory effect. This observation suggested that each Elf-1 isoform played a different role in the gene expression under its control.
Nishiyama
C
C
Foods & Pharmaceuticals Research & Development Laboratory, Asahi Breweries, Ltd., Kitasoma-gun, Ibaraki, Japan. chinishi@med.juntendo.ac.jp
Takahashi
K
K
Nishiyama
M
M
Okumura
K
K
Ra
C
C
Ohtake
Y
Y
Yokota
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D018076:DNA, Complementary; D036383:Ephrin-A2; D017353:Gene Deletion; D005786:Gene Expression Regulation; D006801:Humans; D008969:Molecular Sequence Data; D019154:Protein Splicing; D051381:Rats; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D014158:Transcription, Genetic
D018076:DNA, Complementary; D036383:Ephrin-A2; D011994:Recombinant Proteins; D014157:Transcription Factors; D004247:DNA