[Effect of S-adenosyl-L-methionine and its analogs on site-specific binding DNA-(adenine-N6)-methyltransferase from T4 phage to the oligonucleotide substrate].
Using fluorescence of 2-aminopurine-substituted oligonucleotide duplexes, "flipping" of the target base in the process of interaction of T4 DNA-(adenine-N6)-methyltransferase (EC 2.1.1.72) with the substrate double-stranded DNA was revealed. It was shown that S-adenosyl-L-methionine, the methyl group donor, induces the reorientation of the enzyme relative to the asymmetrically modified recognition site.
Evdokimov
A A
AA
Zinov'ev
V V
VV
Malygin
E G
EG
D017122:Bacteriophage T4 / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D009841:Oligonucleotides; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)
Vliianie S-adenozil-L-metionina i ego analogov na saĭt-spetsificheskoe sviazyvanie DNK-(adenin-N6)-metiltransferazy faga T4 s oligonukleotidnym substratom.