Synthesis and incorporation of pyrrole carboxamide nucleoside triphosphates by DNA polymerases.
We have synthesised and examined the enzymatic incorporation properties of the 5'-triphosphates of 2'-deoxyribosyl pyrrole 3-monocarboxamide (dMTP) and 2'-deoxyribosyl pyrrole 3,4-dicarboxamide (dDTP). These analogues we had hoped would behave as ambivalent base analogues in that they can present two alternative hydrogen-bonding faces either by rotation about the carboxamide group or about the glycosidic bond. The two pyrrole derivatives, dMTP and dDTP, exhibit a preference for incorporation with Klenow polymerase. They are preferentially incorporated as either A or C.
Loakes
D
D
Medical Research Council, Laboratory of Molecular Biology, Cambridge, UK.
Guo
M J
MJ
Brown
D M
DM
Salisbury
S A
SA
Smith
C L
CL
Felix
I
I
Kumar
S
S
Nampalli
S
S
D001483:Base Sequence; D018360:Crystallography, X-Ray; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D009711:Nucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment
D009711:Nucleotides; D011758:Pyrroles; D004259:DNA-Directed DNA Polymerase