title: Anticancer activity of hydrophobic peptides from soy proteins.
abstract: An anticancer peptide from soy protein was purified and isolated. Defatted soy protein was hydrolyzed with thermoase and hydrophobic peptides were extracted with ethanol. The peptide extract was fractionated by XAD-2 hydrophobic, gel filtration chromatography, and different C18 HPLCs. Anticancer activity of each fraction was assayed by measuring in vitro cytotoxicity on P388D1, a mouse monocyte macrophage cell line. IC50 value of a peptide fraction from Sephadex G-25 chromatography was 0.16 mg/ml. This peptide fraction at 1 mg/ml significantly affected cell cycle progression by arresting P388D1 at G2/M phases. Finally purified peptide from analytical C18 HPLC was nonapeptide of which molecular weight was 1157 Da and the sequence was X-Met-Leu-Pro-Ser-Tye-Ser-Pro-Tyr.
authors.affiliation: Department of Food Science and Technology, Seoul National University, Suwon, Korea.
authors.forename: S E
authors.initials: SE
authors.lastname: Kim
authors.forename: H H
authors.initials: HH
authors.lastname: Kim
authors.forename: J Y
authors.initials: JY
authors.lastname: Kim
authors.forename: Y I
authors.initials: YI
authors.lastname: Kang
authors.forename: H J
authors.initials: HJ
authors.lastname: Woo
authors.forename: H J
authors.initials: HJ
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D016195:G2 Phase / Q000187:drug effects; D006868:Hydrolysis; D007941:Leukemia P388 / Q000473:pathology; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D008938:Mitosis / Q000187:drug effects; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D030262:Soybean Proteins / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D010455:Peptides; D030262:Soybean Proteins