title: Different transendothelial migration behaviour pattern of blood monocytes derived from patients with benign and malignant diseases of the breast.
abstract: BACKGROUND
  In this study we compared the expression of selected monocyte surface antigens with the potential to transmigrate through an endothelial layer before and after surgery from breast cancer patients (CA) and patients with benign disease of the breast (BE).
  
  
  METHODS
  Transmigration capacity of mononuclear cells was determined after isolation by Ficoll density gradient, layered over human umbilical vein endothelial cells and cultured in a two chamber plate added with fMLP as a chemotactic stimulus. We determined monocyte phenotye (HLA-DR, FcgRI/CD64, CR1/CD11b and LFA-1/CD11a) and the phagocytosis of E. coli by flow cytometry.
  
  
  RESULTS
  Before surgery blood monocytes had an equal expression of the measured surface antigens, but were different in regard to their interaction with endothelial cells. Monocytes derived from CA had a higher transmigration potency than those of BE. Moreover, the migration through the endothelial cell layer created different populations of monocytes. Surgical stress modified transmigrated monocytes of BE into the direction of monocytes from CA. Phagocytic capacity of peripheral blood monocytes from CA was significantly diminished and was further reduced after surgery when measured in transmigrated cells.
  
  
  CONCLUSIONS
  Our study shows that monocytes from CA and BE can be discriminated in regard to their interaction with endothelial cells.
authors.affiliation: Department of Surgery, University of Vienna, Vienna, Austria.
authors.forename: B
authors.initials: B
authors.lastname: Gebhard
authors.forename: M
authors.initials: M
authors.lastname: Gnant
authors.forename: G
authors.initials: G
authors.lastname: Schütz
authors.forename: S
authors.initials: S
authors.lastname: Roka
authors.forename: G
authors.initials: G
authors.lastname: Weigel
authors.forename: D
authors.initials: D
authors.lastname: Kandioler
authors.forename: S
authors.initials: S
authors.lastname: Taucher
authors.forename: T
authors.initials: T
authors.lastname: Grünberger
authors.forename: E
authors.initials: E
authors.lastname: Roth
authors.forename: R
authors.initials: R
authors.lastname: Jakesz
authors.forename: A
authors.initials: A
authors.lastname: Spittler
mesh_terms: D000328:Adult; D000368:Aged; D000954:Antigens, Surface / Q000032:analysis*; D015415:Biomarkers / Q000032:analysis; D001943:Breast Neoplasms / Q000276:immunology* / Q000503:physiopathology / Q000601:surgery; D018270:Carcinoma, Ductal, Breast / Q000276:immunology* / Q000503:physiopathology / Q000601:surgery; D018275:Carcinoma, Lobular / Q000276:immunology* / Q000503:physiopathology / Q000601:surgery; D002465:Cell Movement / Q000502:physiology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology*; D005260:Female; D018226:Fibroadenoma / Q000276:immunology* / Q000503:physiopathology / Q000601:surgery; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D016169:Lymphocyte Function-Associated Antigen-1 / Q000032:analysis; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology / Q000276:immunology / Q000502:physiology*; D010641:Phenotype; D017463:Receptors, Complement 3b / Q000032:analysis; D017452:Receptors, IgG / Q000032:analysis
chemical_list: D000954:Antigens, Surface; D015415:Biomarkers; D006684:HLA-DR Antigens; D016169:Lymphocyte Function-Associated Antigen-1; D017463:Receptors, Complement 3b; D017452:Receptors, IgG