Mismatch between BDNF mRNA and protein expression in the developing visual cortex: the role of visual experience.
Brain-derived neurotrophic factor (BDNF) messenger RNA (mRNA) expression in the rat visual cortex of young and postnatal day 90 (P90) animals is developmentally regulated and influenced by visual experience. In the present paper we compared the expression of BDNF mRNA to the actual changes of BDNF protein occurring during postnatal development and verified whether BDNF protein distribution is controlled by visual activity. To achieve this aim we analysed BDNF mRNA and/or BDNF protein cellular distribution in the rat visual cortex at different postnatal ages by using immunohistochemistry and highly sensitive in situ hybridization. We found that before eye opening (P13), in all cortical layers a large number of visual cortical neurons contain BDNF mRNA with no detectable amount of BDNF protein. At later ages (P23 and P90), the number of BDNF-immunostained cells increases; most neurons are double labelled for BDNF mRNA and protein, and a small group of neurons is labelled only for BDNF protein. The cellular increase of BDNF immunolabelling is blocked in animals deprived of visual experience from birth (dark rearing), with a large population of neurons containing BDNF mRNA but not BDNF protein. This is similar to what is observed before eye opening. Exposure of dark-reared rats to a brief period (2 h) of light restores a good match between BDNF mRNA and BDNF protein cellular expression. We propose that visual experience controls the neuronal content of BDNF mRNA and BDNF protein in developing visual cortex.
Tropea
D
D
Neuroscience Program, International School for Advanced Studies (SISSA), Via Beirut 2-4, 34014 Trieste, Italy.
Capsoni
S
S
Tongiorgi
E
E
Giannotta
S
S
Cattaneo
A
A
Domenici
L
L
D000818:Animals; D015153:Blotting, Western; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis* / Q000235:genetics; D002452:Cell Count; D003078:Colchicine / Q000008:administration & dosage / Q000494:pharmacology; D003624:Darkness; D018507:Gene Expression Regulation, Developmental; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D007276:Injections, Intraventricular; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D009473:Neuronal Plasticity; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D010320:Parvalbumins / Q000032:analysis; D010775:Photic Stimulation; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D012683:Sensory Deprivation; D016037:Single-Blind Method; D014793:Visual Cortex / Q000254:growth & development / Q000378:metabolism*
D019208:Brain-Derived Neurotrophic Factor; D009419:Nerve Tissue Proteins; D010320:Parvalbumins; D012333:RNA, Messenger; D003078:Colchicine