title: Effect of incident angle of light on sensitivity and detection limit for layers of antibody with surface plasmon resonance spectroscopy.
abstract: The effect of the incident angle of light on sensitivity and the detection limit for surface-plasmon resonance spectroscopy were examined. The sensitivities and the detection limit were experimentally measured using an antibody as a modeled analyte in the incident angles of a light region of 66-76 degrees. The results showed that the sensitivity of a smaller incident angle was higher than that of a larger one. For instance, the sensitivity of a 66 degree incident angle was three times higher than that of a 76 degree incident angle. The detection limit with a 66 degree incident angle was one-tenth of that with a 76 degree incident angle. These sensitivities and detection limits were compared with those of a commercially produced surface-plasmon resonance instrument. This comparison demonstrated that a wavelength resolution of the order of less than 10(-2) nm was necessary to obtain satisfactory sensitivities and detection limits. In addition, the refractive index and thickness of the antibody layer formed on a sensor surface was proposed by experimental results and theoretical calculation.
authors.affiliation: Research Center for Advanced Science and Technology, The University of Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Akimoto
authors.forename: S
authors.initials: S
authors.lastname: Sasaki
authors.forename: K
authors.initials: K
authors.lastname: Ikebukuro
authors.forename: I
authors.initials: I
authors.lastname: Karube
mesh_terms: D000327:Adsorption; D000906:Antibodies / Q000032:analysis*; D008027:Light; D012031:Refractometry; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance
chemical_list: D000906:Antibodies