The circumoval precipitin reaction in Fasciola. Detection of shell pores by scanning electron microscopy.
Precipitation reaction was detected around Fasciola eggs containing mature miracidia when they were incubated for 20 minutes with the sera of acute and chronic fasciolosis patients. The reaction was of the bleb type. The amount of blebs formed around the eggs was greater in case of acute than chronic fasciolosis. Scanning electron microscopy illustrated pores in the shell of Fasciola eggs. It is probable that the metabolic antigen passes through these pores leading to the positive circumoval precipitin reaction in cases of Fasciola infection. Antibodies specific to Fasciola excretory/secretory (E/S) egg antigens were not detected in the sera of Schistosoma cases.
el-Sayad
M H
MH
Department of Parasitology, Medical Research Institute, Alexandria University, Alexandria, Egypt.
Allam
A F
AF
Shehab
A Y
AY
D000818:Animals; D005209:Fasciola / Q000302:isolation & purification / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning; D011233:Precipitin Tests