[Structure and physiological activity of lemnan, Lemna minor L. pectin].
A pectic polysaccharide, lemnan, was isolated from freshly collected duckweed Lemna minor L. Its sugar chain was shown to be mainly composed of the residues of D-galacturonic acid (64%), galactose, arabinose, xylose, and D-apiose, a branched chain sugar. The high content of D-apiose (25%) indicated that lemnan is an apiogalacturonan type pectin similar to zosteran, a pectic polysaccharide from a sea phanerogam of the Zosteraceae family. The results of partial acidic hydrolysis, pectinase digestion, and NMR studies of lemnan demonstrated that its macromolecule contains regions of the linear alpha-1,4-D-galacturonan and branched apiogalacturonan. The side chains of apiogalacturonan were found to be formed of single and 1,5-linked residues of D-apiofuranose attached to 2- and 3-positions of the D-galacturonic acid residues of the apiogalacturonan backbone. Lemnan was shown to exhibit an immunomodulatory effect by activating the system of phagocytosis.
Ovodova
R G
RG
Institute of Physiology, Komi Science Center, Ural Branch, Russian Academy of Sciences, ul. Pervomaiskaya 50, Syktyvkar, 167982 Russia. ovoys@physiol.komisc.ru
Golovchenko
V V
VV
Shashkov
A S
AS
Popov
S V
SV
Ovodov
Iu S
IuS
D000276:Adjuvants, Immunologic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D066298:In Vitro Techniques; D007928:Lethal Dose 50; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051379:Mice; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D010368:Pectins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D010587:Phagocytosis; D010944:Plants / Q000737:chemistry*
D000276:Adjuvants, Immunologic; C423783:lemnan; D010368:Pectins
Strukturnoe issledovanie i fiziologicheskaia aktivnost' lemnana, pektina iz Lemna minor L..