Simultaneous quantitative determination of norgestrel and progesterone in human serum by high-performance liquid chromatography-tandem mass spectrometry with atmospheric pressure chemical ionization.
A selective, reliable and rapid method for the simultaneous determination of progesterone and norgestrel concentrations in human serum after taking oral contraceptive tablet has been developed using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS-MS) with atmospheric pressure chemical ionization (APCI) interface. The norethisterone was used as the internal standard. Selected transitions of m/z 313/245 for norgestrel, m/z 315/97 for progesterone and m/z 299/109 for norethisterone were monitored using multiple reaction monitoring (MRM) mode for quantitation. The assay was linear over the concentration range of 0.2-50 ng mL-1 for norgestrel and progesterone. The lower level of quantitation in human serum was obtained at 0.2 ng mL-1 for both norgestrel and progesterone using optimum tuning parameters. The intra-assay precision and inter-assay precision do not exceed 10 and 9%, respectively. The method has been applied to the determination of norgestrel and progesterone in serum of female volunteers.
Wu
Z
Z
Department of Chemistry, Tsinghua University, Beijing 100084, China.
Zhang
C
C
Yang
C
C
Zhang
X
X
Wu
E
E
D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003280:Contraceptives, Oral, Synthetic / Q000097:blood*; D005260:Female; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D009644:Norgestrel / Q000097:blood*; D011374:Progesterone / Q000097:blood*; D012680:Sensitivity and Specificity
D003280:Contraceptives, Oral, Synthetic; D009644:Norgestrel; D011374:Progesterone