HLA class I haplotypes in families of children with coeliac disease.
The purpose of the study was the analysis of HLA-AB haplotypes frequency in the families of children with coeliac disease. Haplotypes present in 46 probands' families including 69 affected children, 49 healthy siblings and 91 parents were verified. HLA antigens were typed by Terasaki and McClelland's routine two-step-microcytotoxic assay in NIH modification. Among 138 haplotypes, the following were significantly more frequent in affected children: HLA-A1-B8 (3116 x 10(4)), HLA-A3-B8 (290 x 10(4)) and HLA-A2-B8 (217 x 10(4)). Total frequency of haplotypes including HLA-B8 antigen in comparison to control population equalled 3986 x 10(4) vs. 763 x 10(4). HLA-A1-B8 haplotype frequency was twice lower in probands' healthy siblings and parents, equalling 1837 x 10(4) and 1868 x 10(4), respectively. Highly significantly more frequent HLA-A1-B8 haplotype found in probands' families may indicate the correlation between inherited gene products and increased risk of coeliac disease incidence.
Kedzierska
A
A
Independent Laboratory of Clinical Immunology, Faculty of Medicine, Collegium Medicum, Jagiellonian University, ul. Grzegórzecka 16, 31-531 Cracow, Poland.
Turowski
G
G
D016022:Case-Control Studies; D002446:Celiac Disease / Q000235:genetics* / Q000276:immunology*; D002648:Child; D005260:Female; D005787:Gene Frequency; D005805:Genes, MHC Class I; D015234:HLA-A Antigens / Q000235:genetics*; D015235:HLA-B Antigens / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D008297:Male; D009679:Nuclear Family; D010290:Parents
D015234:HLA-A Antigens; D015235:HLA-B Antigens