title: Unilateral intrauterine horn insemination of frozen semen in cats.
abstract: Frozen feline semen was prepared using two types of extenders, egg yolk Tris-fructose citric acid (EYT-FC) and egg yolk sodium citrate solution (EYC), and the semen qualities after thawing and the conception rates obtained by unilateral intrauterine horn insemination (UIUI) were investigated. Cats used in the experiment were six males and 11 females aged 2-12 years (the number of experimental cases was 17). For preparation of frozen semen, semen collected by the artificial vagina method was adjusted to I x 10(8) sperm/m/ and 7% glycerol, put in 250 microl straws, and then frozen using a cell freezer. The mean sperm motility after thawing was 30.0+/-9.7 (SE) % in the semen prepared with EYT-FC and 30.0+/-3.3% in the semen prepared with EYC. Four of seven animals were fertilized by UIUI using two straws in both extenders, and the conception rate was 57.1%. The mean ratios of number of kits to the number of ovulations in the inseminated side were 61.1+/-24.5% and 30.5+/-3.4% for EYT-FC and EYC, respectively, showing that the ratio tended to be higher in the semen prepared with EYT-FC. The above findings, comparing the two extenders for preparation of frozen feline semen, showed that EYT-FC is slightly superior to EYC. To increase conception and fertility rates, it may be important to increase the sperm count for insemination and to inseminate both uterine horns.
authors.affiliation: Department of Reproduction, Nippon Veterinary and Animal Science University, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Tsutsui
authors.forename: A
authors.initials: A
authors.lastname: Tanaka
authors.forename: Y
authors.initials: Y
authors.lastname: Takagi
authors.forename: K
authors.initials: K
authors.lastname: Nakagawa
authors.forename: Y
authors.initials: Y
authors.lastname: Fujimoto
authors.forename: M
authors.initials: M
authors.lastname: Murai
authors.forename: M
authors.initials: M
authors.lastname: Anzai
authors.forename: T
authors.initials: T
authors.lastname: Hori
mesh_terms: D000818:Animals; D002415:Cats / Q000502:physiology*; D006063:Chorionic Gonadotropin / Q000008:administration & dosage; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D005260:Female; D005306:Fertilization / Q000502:physiology*; D007315:Insemination, Artificial / Q000379:methods / Q000662:veterinary*; D008098:Litter Size; D008297:Male; D006080:Ovarian Follicle / Q000502:physiology; D010062:Ovulation Induction / Q000662:veterinary; D011247:Pregnancy; D012661:Semen / Q000502:physiology; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility; D014463:Ultrasonography; D014599:Uterus / Q000000981:diagnostic imaging / Q000502:physiology
chemical_list: D006063:Chorionic Gonadotropin