title: [Facultative and obligate aerobic methylobacteria synthesize cytokinins].
abstract: The presence and expression of genes controlling the synthesis and secretion of cytokinins by the pink-pigmented facultative methylotroph Methylobacterium mesophilicum VKM B-2143 with the serine pathway and nonpigmented obligate methylotroph Methylovorus mays VKM B-2221 with the ribulose monophosphate pathway of C1 metabolism were shown using the polymerase chain reaction (PCR) and reverse transcription-PCR methods. The presence of the corresponding mRNA in M. mesophilicum cells grown on methanol or succinate suggests that the expression of these genes is constitutive. The cytokinin activity of culture liquid and its fractions was determined by a biotest with Amarantus caudatus L. seedlings. Using enzyme-linked immunosorbent analysis, we detected zeatin (riboside) in the culture liquid of both bacteria studied. The data obtained show that the aerobic methylobacteria are phytosymbionts that are able to utilize the single- and polycarbon compounds secreted by symbiotic plants and to synthesize cytokinins.
authors.affiliation: Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, Pushchino, Moscow oblast, 142290 Russia.
authors.forename: E G
authors.initials: EG
authors.lastname: Ivanova
authors.forename: N V
authors.initials: NV
authors.lastname: Doronina
authors.forename: A O
authors.initials: AO
authors.lastname: Shepeliakovskaia
authors.forename: A G
authors.initials: AG
authors.lastname: Laman
authors.forename: F A
authors.initials: FA
authors.lastname: Brovko
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Trotsenko
mesh_terms: D001483:Base Sequence; D003470:Culture Media; D003583:Cytokinins / Q000096:biosynthesis*; D017931:DNA Primers; D020580:Methylobacterium / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D003470:Culture Media; D003583:Cytokinins; D017931:DNA Primers; D012329:RNA, Bacterial; D012333:RNA, Messenger
vernacular_title: Fakul'tativnye i obligatnye aérobnye metilobakterii sinteziruiut tsitokininy.