Hexokinase 'binding sites' of normal and tumoral human brain mitochondria.
Interaction of type I hexokinase (HK-I) with the mitochondria obtained from the biopsy specimens of normal and tumoral human brain tissues was studied in the present investigation. This effort was undertaken with the aim of exploring possible differences in the mode of association of the enzyme with the outer mitochondrial membrane in the described sources. Results indicate that the two 'sites' for binding of HK-I suggested in the literature, based on extensive studies carried out on rat brain mitochondria, are similarly present in the human brain mitochondria. Differences in the microenvironments of HK binding, as reflected by the presented data, are suggested to be of importance in regulation of the catalytic potential of the bound enzyme. The real metabolic significance of this association in relation to cancer and its practical importance would need further investigation.
Golestani
A
A
Institute of Biochemisty and Biophysics, University of Tehran, Iran.
Nemat-Gorgani
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000201:enzymology* / Q000378:metabolism; D001932:Brain Neoplasms / Q000201:enzymology* / Q000378:metabolism; D020134:Catalytic Domain; D019298:Glucose-6-Phosphate / Q000378:metabolism; D006593:Hexokinase / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology* / Q000378:metabolism; D008954:Models, Biological; D008969:Molecular Sequence Data; D011485:Protein Binding; D051381:Rats; D017386:Sequence Homology, Amino Acid; D013861:Thiocyanates / Q000494:pharmacology; D014018:Tissue Distribution
D013861:Thiocyanates; D019298:Glucose-6-Phosphate; D006593:Hexokinase; C009941:potassium thiocyanate