title: [Purification and biochemical characterization of high-molecular-weight-glutenin subunits 14 and 15].
abstract: The high molecular weight glutenin subunits 14 and 15 were purified from cultivars of bread wheat (Triticum aestivum) Xiaoyan 6 by preparative sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) appled a new method for visualizing protein in gels. N-terminal amino acid sequences were homologous comparing with other High-Molecular-Weight glutenin subunits. The result suggested that they were basic protein analyzed by two-dimensional electrophoresis of IEF(Isoelectric Focussing) x SDS-PAGE and NEPHGE(Non-Equilibrium PI-gradient Electrophoresis) x SDS-PAGE.
authors.affiliation: Northwestern Agricultural-Forestry University of Science & Technical, Yangling Shaanxi 712100, China.
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Deng
authors.forename: H X
authors.initials: HX
authors.lastname: Zhao
authors.forename: S H
authors.initials: SH
authors.lastname: Fan
authors.forename: W Q
authors.initials: WQ
authors.lastname: Ji
authors.forename: A G
authors.initials: AG
authors.lastname: Guo
authors.forename: X Z
authors.initials: XZ
authors.lastname: Xue
mesh_terms: D000595:Amino Acid Sequence; D015180:Electrophoresis, Gel, Two-Dimensional; D005983:Glutens / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D008970:Molecular Weight; D021122:Protein Subunits
chemical_list: D021122:Protein Subunits; D005983:Glutens; C037302:glutenin