title: Development of an ex vivo model to investigate the effects of altered haemodynamics on human bypass grafts.
abstract: The insertion of vein grafts into the arterial circulation may contribute to vessel wall thickening and accelerated atherosclerosis, a common feature of late vein graft failure. We aimed to develop a model suitable for investigation of the effects of altered haemodynamics on human saphenous vein following its implantation into the arterial circulation. Segments of human saphenous vein obtained from patients undergoing coronary artery bypass surgery were sutured at each end to PTFE and placed into a flow system. Pressure and flow rates to stimulate the arterial and venous systems were achieved. A theoretical model of the flow chamber was created and computational fluid dynamics software (FLOTRAN, Swanson Analysis Systems) was used to determine the flow profile within the model. In summary, a flow model has been developed to investigate the effect of altered haemodynamics on the molecular and pathological changes that occur in vein grafts incorporated into the arterial circulation.
authors.affiliation: Cardiovascular Research Group, University of Sheffield Division of Clinical Sciences, Northern General Hospital, Sheffield, UK.
authors.forename: J
authors.initials: J
authors.lastname: Armstrong
authors.forename: A J
authors.initials: AJ
authors.lastname: Narracott
authors.forename: R
authors.initials: R
authors.lastname: Milton
authors.forename: J
authors.initials: J
authors.lastname: Galea
authors.forename: G J
authors.initials: GJ
authors.lastname: Cooper
authors.forename: P V
authors.initials: PV
authors.lastname: Lawford
authors.forename: D R
authors.initials: DR
authors.lastname: Hose
authors.forename: D C
authors.initials: DC
authors.lastname: Cumberland
authors.forename: C M
authors.initials: CM
authors.lastname: Holt
mesh_terms: D001783:Blood Flow Velocity; D001794:Blood Pressure; D002404:Catheterization / Q000295:instrumentation / Q000379:methods; D001026:Coronary Artery Bypass / Q000295:instrumentation* / Q000379:methods*; D006439:Hemodynamics; D006801:Humans; D066298:In Vitro Techniques; D008955:Models, Cardiovascular; D010477:Perfusion / Q000295:instrumentation / Q000379:methods; D011138:Polytetrafluoroethylene; D012501:Saphenous Vein / Q000503:physiopathology; D013314:Stress, Mechanical
chemical_list: D011138:Polytetrafluoroethylene