title: Postmortem absorption of dichloromethane: a case study and animal experiments.
abstract: A case of accidental death after occupational exposure to an atmosphere containing dichloromethane (DCM) is reported. The concentrations of DCM in the blood and tissues of a 40-year-old man who died while observing an industrial washing machine filled with DCM vapour were blood 1660 mg/l, urine 247 mg/l, brain 87 mg/kg, heart muscle 199 mg/kg and lungs 103 mg/kg which are 3-7 times higher than previously reported fatal levels. The body was left undiscovered in the machine filled with DCM vapour for about 20 h. The present study was designed to determine whether all the DCM detected in the tissues and body fluids had been inhaled while alive using rats as the experimental model. The concentrations of DCM in the tissues and body fluids of a rat that died from DCM poisoning and was left for 20 h in a box containing DCM vapour were the same as those in the tissues and body fluids of a rat that had died from an injected overdose of barbiturates and had then been placed in the DCM box in a similar manner. Moreover, the concentrations of DCM in the tissues and body fluids of the carcasses that were exposed to the DCM vapour increased gradually throughout the period of exposure. These findings imply that DCM is able to penetrate the tissues and body fluids of rat carcasses through a route other than inhalation such as through the skin.
authors.affiliation: Department of Legal Medicine, Gunma University School of Medicine, Maebashi, Gunma, 371-8511 Japan.
authors.forename: H
authors.initials: H
authors.lastname: Takeshita
authors.forename: K
authors.initials: K
authors.lastname: Mogi
authors.forename: T
authors.initials: T
authors.lastname: Yasuda
authors.forename: S
authors.initials: S
authors.lastname: Mori
authors.forename: Y
authors.initials: Y
authors.lastname: Nakashima
authors.forename: T
authors.initials: T
authors.lastname: Nakajima
authors.forename: H
authors.initials: H
authors.lastname: Akuzawa
authors.forename: S
authors.initials: S
authors.lastname: Nakajo
authors.forename: Y
authors.initials: Y
authors.lastname: Hirota
authors.forename: K
authors.initials: K
authors.lastname: Kishi
mesh_terms: D000328:Adult; D000818:Animals; D001344:Autopsy / Q000379:methods*; D002263:Carboxyhemoglobin / Q000378:metabolism; D004780:Environment, Controlled; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008297:Male; D008752:Methylene Chloride / Q000506:poisoning* / Q000633:toxicity*; D016273:Occupational Exposure / Q000032:analysis*; D011180:Postmortem Changes; D051381:Rats; D017208:Rats, Wistar; D012869:Skin Absorption; D014018:Tissue Distribution
chemical_list: D008752:Methylene Chloride; D002263:Carboxyhemoglobin