Burn injuries in rats upregulate the gene expression of the ubiquitin-conjugating enzyme E2(14k) in skeletal muscle.
Burn injuries are associated with muscle cachexia, which mainly reflects protein breakdown in the ubiquitin-proteasome pathway. Ubiquitination of proteins degraded by this mechanism is regulated by multiple enzymes, including the 14-kd ubiquitin-conjugating enzyme, E2(14k). In this study, burn injuries in rats resulted in increased levels of the 1.2 kilobase E2(14k) transcript in the white, fast-twitch extensor digitorum longus muscle with no changes or only minor changes in the red, slow-twitch soleus muscle, liver, and kidney. The results provide the first evidence that burn injuries upregulate the gene expression of E2(14k) in skeletal muscle and suggest that ubiquitin-proteasome-dependent muscle protein breakdown after thermal injuries may, at least in part, be regulated by E2(14k).
Fang
C H
CH
Shriners Hospitals for Children, Cincinnati, Ohio, USA.
Sun
X
X
Li
B G
BG
Fischer
D R
DR
Pritts
T A
TA
Penner
G
G
Hasselgren
P O
PO
D000818:Animals; D002056:Burns / Q000150:complications*; D002100:Cachexia / Q000503:physiopathology; D005786:Gene Expression Regulation; D008025:Ligases / Q000096:biosynthesis* / Q000378:metabolism; D008297:Male; D018482:Muscle, Skeletal / Q000201:enzymology / Q000473:pathology*; D011506:Proteins / Q000378:metabolism; D051381:Rats; D044763:Ubiquitin-Conjugating Enzymes; D015854:Up-Regulation
D011506:Proteins; D044763:Ubiquitin-Conjugating Enzymes; D008025:Ligases