Synthesis and RNAse L binding and activation of a 2-5A-(5')-DNA-(3')-PNA chimera, a novel potential antisense molecule.
Fully automated solid-phase synthesis gave access to a hybrid in which 5'-phosphorylated-2'-5'-linked oligoadenylate (2-5A) is connected to the 5'-terminus of DNA which, in turn, is linked at the 3'-end to PNA [2-5A-(5')-DNA-(3')-PNA chimera]. This novel antisense molecule retains full RNase L activation potency while suffering only a slight reduction in binding affinity.
Verheijen
J C
JC
Leiden Institute of Chemistry, Gorlaeus Laboratories, The Netherlands.
Chen
L
L
Bayly
S F
SF
Torrence
P F
PF
van der Marel
G A
GA
van Boom
J H
JH
D002851:Chromatography, High Pressure Liquid; D004722:Endoribonucleases / Q000138:chemical synthesis* / Q000378:metabolism; D004789:Enzyme Activation; D006801:Humans; D016376:Oligonucleotides, Antisense / Q000378:metabolism*; D020135:Peptide Nucleic Acids; D011485:Protein Binding
D016376:Oligonucleotides, Antisense; D020135:Peptide Nucleic Acids; D004722:Endoribonucleases; C031956:2-5A-dependent ribonuclease