Identification of an organic anion transport system in the human colon carcinoma cell line HT29 clone 19A.
The human colon carcinoma cell line HT29 c1.19A was studied for organic anion transporter activity by determining intracellular fluo-3 and fura-red accumulation and by measuring fluo-3 efflux. Modulators of organic anion transport systems were used to identify the transporters that are involved in dye extrusion. Addition of probenecid to the dye-loading medium, containing 10 microM fluo-3/AM and fura-red/AM, resulted in a dose-dependent increase in fluo-3 and fura-red accumulation in the cells. The increase in fluo-3 accumulation in the cells in the presence of probenecid was explained by the inhibitory effect of this compound on fluo-3 efflux. Fluo-3 efflux from the cells was also inhibited by sulfinpyrazone, another inhibitor of organic anion transport. Substrates of renal probenecid-sensitive organic anion exchange mechanisms as well as modulators of multidrug resistance associated protein (MRP) activity did not influence fluo-3 extrusion rates. However, reducing intracellular ATP contents completely blocked fluo-3 extrusion. Moreover, MK571, an inhibitor of MRP, significantly stimulated dye accumulation, whereas inhibitors of the multidrug resistance gene (MDR1) product Pglycoprotein, cyclosporin A and verapamil, did not. As probenecid inhibits fluo-3 efflux across the apical membrane of cells grown on permeable supports, we conclude that a probenecid-sensitive organic anion transporter is present in the apical membrane of HT29 c1.19A cells. This organic anion transport system differs from MDRI and MRP2.
Abrahamse
S L
SL
Surgical Laboratory, Academic Medical Center, Amsterdam, The Netherlands. s.l.abrahamse@amc.uva.nl
Rechkemmer
G
G
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000255:Adenosine Triphosphate / Q000378:metabolism; D000814:Aniline Compounds / Q000378:metabolism; D000838:Anions; D001572:Benzofurans / Q000378:metabolism; D001692:Biological Transport / Q000187:drug effects; D002352:Carrier Proteins / Q000378:metabolism*; D017097:Electric Impedance; D005456:Fluorescent Dyes / Q000378:metabolism; D019073:HT29 Cells; D006801:Humans; D007093:Imidazoles / Q000378:metabolism; D008564:Membrane Potentials; D008856:Microscopy, Fluorescence; D011339:Probenecid / Q000494:pharmacology; D014966:Xanthenes / Q000378:metabolism
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000814:Aniline Compounds; D000838:Anions; D001572:Benzofurans; D002352:Carrier Proteins; D005456:Fluorescent Dyes; D007093:Imidazoles; D014966:Xanthenes; C082877:fura red; C059715:Fluo-3; D000255:Adenosine Triphosphate; D011339:Probenecid