[Plasminogen activation by antiplasminogen monoclonal antibody IV-1C. Properties and mechanism of reaction].
In review the results of investigation of plasminogen(Pg) activation by antiplasminogen monoclonal antibody IV-1c have been presented. Antigenic determinant of IV-1c was localized in Val709-Gly718 site of Pg protease domain. IV-1c completely inhibited the Pg activation by streptokinase, but increased the rate of Pg activation by t-PA and urokinase. Catalytic properties of plasmin in complex with IV-1c were studied. It was found that IV-1c induced catalytic activity in Pg-IV-1c complex. It was shown that Pg and IV-1c interacts in complex by two-centre mechanism: IV-1c binds with Pg by paratope and by N-terminal lysine of gamma-chain and Pg binds to IV-1c by one of the lysine binding sites and by V709-G718 site of protease domain. The influence of pH, temperature, 1.5 mM Ca2+, Mg2+, Sr2+, Ba2+, Co2+, Ni2+ cations and 10 mM Cl-, F-, Ac-, SO4(2-), HPO4(2-) anions on lag and fast phases of Pg activation by VI-1c was investigated. It was revealed that Val709-Gly718 site was determining in Pg activation by IV-1c and streptokinase.
Makohonenko
Ie M
IeM
Palladine Institute of Biochemistry, NAS of Ukraine, Kyiv. emm@biochem.kiev.ua
Iakovlev
S O
SO
Slomins'kyĭ
O Iu
OIu
Korol'chuk
V I
VI
Hrynenko
T V
TV
Sokolovs'ka
L A
LA
Druzhyna
N M
NM
Kolesnykova
I M
IM
Chernyshov
V I
VI
Sederkhol'm-Vil'iams
S A
SA
D000595:Amino Acid Sequence; D000838:Anions; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology*; D002384:Catalysis; D002413:Cations, Divalent; D000939:Epitopes / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010958:Plasminogen / Q000276:immunology* / Q000378:metabolism*
D000838:Anions; D000911:Antibodies, Monoclonal; D002413:Cations, Divalent; D000939:Epitopes; D010958:Plasminogen
Aktyvatsiia plazminhenu antyplazminohenovym monoklonal;nym antytilom IV-1c. Vlastyvosti ta mekhanizm reaktsiï