Lactate dehydrogenase from the northern krill Meganyctiphanes norvegica: comparison with LDH from the Antarctic krill Euphausia superba.
Electrophoretic polymorphism of lactate dehydrogenase (LDH, EC 1.1.1.27) from abdominal muscle is reported in the northern krill Meganyctiphanes norvegica. In the population, from the Gullmarsfjord (west coast of Sweden), LDH was encoded for by two different Ldh-A* and -B* loci. The isoenzymes were named according to their electrophoretic mobilities. Ldh-A* locus was polymorphic. The allelic frequencies were a=0.99, a'=0.002, a"=0.004, a"'=0.004. The level of LDH polymorphism is low. Most individuals possess the same amount of two LDH homopolymers (LDH-A*(4) and LDH-B*(4)). The Meganyctiphanes norvegica LDH-A*(4) and LDH-B*(4) isoenzymes and the predominant LDH-A*(4) isoenzyme from Euphausia superba were purified to specific activities of 294, 306 and 464 micromol NADH min(-1) mg(-1), respectively. In both species the LDH isoenzymes were separated by chromatofocusing. All three isoenzymes are L-specific tetramers with molecular weight of approximately 160 kDa. Northern krill LDH-A*(4) has higher affinity for pyruvate and lactate and is more thermostable than LDH-B*(4). Both isoenzymes are inhibited significantly by high concentration of pyruvate but not lactate. Antarctic krill isoenzyme exhibits high substrate affinities, high NAD inhibition, high inhibition at 10 mM pyruvate, lack of lactate inhibition, and high heat stability and resembles northern krill LDH-A*(4) isoenzyme.
Mulkiewicz
E
E
Gdańsk University Biological Station, 80-680 Gdańsk, Poland.
Zietara
M S
MS
Strömberg
J O
JO
Skorkowski
E F
EF
D000483:Alleles; D000818:Animals; D002850:Chromatography, Gel; D003445:Crustacea; D004591:Electrophoresis, Polyacrylamide Gel; D005787:Gene Frequency; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000737:chemistry; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000737:chemistry* / Q000235:genetics*; D019344:Lactic Acid / Q000494:pharmacology; D009132:Muscles / Q000201:enzymology; D011110:Polymorphism, Genetic; D011485:Protein Binding; D011773:Pyruvates / Q000494:pharmacology; D012685:Sepharose / Q000378:metabolism; D013045:Species Specificity; D013696:Temperature
D007527:Isoenzymes; D011773:Pyruvates; D019344:Lactic Acid; D012685:Sepharose; D007770:L-Lactate Dehydrogenase