Speciation of antimony(III) and antimony(V) in cell extracts by anion chromatography/inductively coupled plasma mass spectrometry.
An analytical method for the separation and quantification of Sb(III) and Sb(V) using anion chromatography with ICP-MS is presented. The optimum conditions for the separation of the antimony species were established with 15 mmol/L nitric acid at pH 6 as eluent system on a PRP-X100 column. The retention times for antimony(V) and antimony(III) were 85 s and 300 s with detection limits of 0.06 microg/L and 0.29 microg/L, respectively. The proposed method was applied to cell extracts of Leishmania donovani, which were incubated with antimony(III) and antimony(V). Some metabolism seemed to occur within the cells.
Ulrich
N
N
University of Hannover, Department of Inorganic Chemistry, Germany.
Shaked
P
P
Zilberstein
D
D
D000818:Animals; D000965:Antimony / Q000032:analysis* / Q000378:metabolism / Q000627:therapeutic use; D002457:Cell Extracts / Q000737:chemistry; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004351:Drug Resistance; D006863:Hydrogen-Ion Concentration; D007893:Leishmania donovani / Q000737:chemistry* / Q000166:cytology / Q000254:growth & development; D007896:Leishmaniasis / Q000188:drug therapy / Q000469:parasitology; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D017942:Nitric Acid; D010795:Phthalic Acids
D002457:Cell Extracts; D010795:Phthalic Acids; D017942:Nitric Acid; D000965:Antimony