Unusual lipid composition of a Bacillus sp. isolated from Lake Pomorie in Bulgaria.
The lipid composition of a Bacillus sp., isolated from Lake Pomorie in Bulgaria, was unusual and consisted of 26 different fatty acids between C12 and C26, with anteiso C15-C17 saturated fatty acids predominating. The furan fatty acid, 10,13-epoxy-11-methyloctadeca-10,12-dienoic acid, was also identified, a new finding for this genus. The hydrocarbons consisted of 30 different monounsaturated hydrocarbons, between C25 and C30, with the iso-iso, iso-anteiso, anteiso-anteiso, iso-normal, and anteiso-normal methyl branching for odd-numbered chains, and the iso-iso, iso-anteiso, iso-normal, and anteiso-normal methyl branching for even-numbered chains. The double bond positions in these hydrocarbons were determined by dimethyl disulfide derivatization followed by GC-MS, and the double-bond cis configuration was confirmed by infrared spectroscopy. Some previously unknown hydrocarbons in bacteria, such as (Z)-3,21-dimethyl-9-tricosene, (Z)-3,21-dimethyl-10-tricosene, (Z)-2,24-dimethyl-11-pentacosene, and (Z)-2,25-dimethyl-13-hexacosene were identified. Sterols were detected and were based on the sitosterol nucleus.
Carballeira
N M
NM
Department of Chemistry, University of Puerto Rico, San Juan 00931, USA. ncarball@upracd.upr.clu.edu
Guzmán
A
A
Nechev
J T
JT
Lahtchev
K
K
Ivanova
A
A
Stefanov
K
K
D001407:Bacillus / Q000737:chemistry*; D002031:Bulgaria; D004220:Disulfides / Q000737:chemistry; D005227:Fatty Acids / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D006838:Hydrocarbons / Q000737:chemistry; D008041:Linoleic Acids / Q000145:classification / Q000378:metabolism; D008055:Lipids / Q000737:chemistry*; D013055:Spectrophotometry, Infrared
D004220:Disulfides; D005227:Fatty Acids; D006838:Hydrocarbons; D008041:Linoleic Acids; D008055:Lipids; C096139:10,13-epoxy-11-methyloctadeca-10,12-dienoic acid