The cycle of the seminiferous epithelium in the greater Japanese shrew mole, Urotrichus talpoides.
Spermatogenesis and acrosomal formation in the greater Japanese shrew mole, Urotrichus talpoides, were studied by light microscopy. On the basis of acrosomal changes, morphology of spermatid head, nuclear shape, appearance of meiotic figures, location of spermatid and period of spermiation, the cycle of the seminiferous epithelium was classified into 12 stages, and developing spermatids could be divided into 15 steps. The mean relative frequencies of stages from I to XII were 10.9, 8.7, 9.8, 7.3, 8.5, 10.3, 12.5, 8.7, 5.8, 5.4, 5.1 and 7.1%, respectively. Similar to the case in the musk shrew, the spermatid nucleus of the greater Japanese shrew mole remained in the middle region of the seminiferous epithelium and only the acrosome extended towards the basement membrane. The elongation of the acrosome, however, was not prominent. The proacrosomal vesicle first appeared in stage II and then one large and round granule was seen in stage III. The acrosomal vesicle became flattened on the surface of the nucleus in stage IV. Spreading of the acrosomic system has been recognized from stage VII. In stage VII, spermiation occurred. In stage IX, the spermatid nucleus began to elongate. Elongation and condensation of the nucleus were clearly observed in stage X. In stage XII, pachytene spermatocytes divided into diplotene spermatocytes. In stage XII, meiotic figures and secondary spermatocytes were observed.
Mizukam
T
T
Laboratory of Anatomy and Physiology, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan.
Kuwahara
S
S
Ohmura
M
M
Iinuma
Y
Y
Izumikubo
J
J
Hagiwara
M
M
Kurohmaru
M
M
Hayashi
Y
Y
Nishida
T
T
D000177:Acrosome / Q000648:ultrastructure; D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D004847:Epithelial Cells / Q000166:cytology / Q000502:physiology; D008297:Male; D008971:Moles / Q000502:physiology*; D012671:Seminiferous Tubules / Q000166:cytology* / Q000502:physiology*; D013045:Species Specificity; D013087:Spermatids / Q000166:cytology / Q000502:physiology; D013091:Spermatogenesis; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology*