[Effect of preparations of protein Yop encoded by Yersinia pestis calcium-dependence plasmid on mice].
The impact of the preparations of Y. pestis secreted proteins Yop (YopH-M, YopB, YopD-N, YopE) on mice immunized with 3 s.c. injections was studied. Though these proteins failed to protect the animals from plague, they stimulated the immunobiological transformation in the immunized animals. YopB and YopD-N were found to have the highest immunobiological activity with respect to mice. The preparation of YopB induced the production of the highest titers of specific antibodies and stimulated cell-mediated immune response. The injection of YopD-N to mice led to a considerable decrease in the proliferative capacity of splenocytes in vitro in response to stimulation with nonspecific mitogen ConA, as well as to pathological changes in the kidneys.
Mikerov
A N
AN
Research Institute for Plague Control Microbe, Saratov, Russia.
Emel'ianova
N V
NV
Nazarova
L S
LS
Vidiaeva
N A
NA
Kutyrev
V V
VV
D000818:Animals; D000917:Antibody Formation; D001425:Bacterial Outer Membrane Proteins / Q000009:adverse effects / Q000235:genetics / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D007111:Immunity, Cellular; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D010930:Plague / Q000276:immunology / Q000401:mortality; D010957:Plasmids; D017027:Protein Tyrosine Phosphatases / Q000009:adverse effects / Q000235:genetics / Q000494:pharmacology; D013154:Spleen / Q000187:drug effects / Q000473:pathology; D015010:Yersinia pestis / Q000235:genetics / Q000378:metabolism*
D001425:Bacterial Outer Membrane Proteins; C078909:YopB protein, Yersinia; C078910:YopD protein, Yersinia; C060772:yopM protein, Yersinia; D017027:Protein Tyrosine Phosphatases; C067571:yopH protein, Yersinia; D002118:Calcium
Deĭstvie na mysheĭ preparatov belkov Yop, kodiruemykh plazmidoĭ kal'tsiĭzavisimosti Yersinia pestis.