title: Mitochondrial DNA analysis of Sporothrix schenckii in South Africa and Australia.
abstract: Clinical isolates of Sporothrix schenckii were investigated for mitochondrial DNA (mtDNA) types using restriction fragment length polymorphism patterns with HaeIII. The 62isolates in South Africa comprised Types 3 (9.7%), 4 (1.6%), 11 (9.7%), 17 (77.4%) and 23 (1.6%) while the 23 Australian isolates comprised Types 3 (26.0%), 4 (56.5%), 7 (8.6%) and 21 (8.6%). In a phylogenetic tree based on the sequence divergence of mtDNA, the mtDNA types were clustered into two groups, A and B. The results suggested that isolates in South Africa mainly belong to Group A and isolates in Australia mainly belong to Group B.
authors.affiliation: Department of Dermatology, Kanazawa Medical University, Ishikawa, Japan. i64-3397@kanazawa-med.ac.jp
authors.forename: H
authors.initials: H
authors.lastname: Ishizaki
authors.forename: M
authors.initials: M
authors.lastname: Kawasaki
authors.forename: M
authors.initials: M
authors.lastname: Aoki
authors.forename: H
authors.initials: H
authors.lastname: Vismer
authors.forename: D
authors.initials: D
authors.lastname: Muir
mesh_terms: D001315:Australia / Q000453:epidemiology; D004271:DNA, Fungal / Q000032:analysis*; D004272:DNA, Mitochondrial / Q000032:analysis*; D015252:Deoxyribonucleases, Type II Site-Specific; D006801:Humans; D017720:Molecular Epidemiology; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D013019:South Africa / Q000453:epidemiology; D013175:Sporothrix / Q000145:classification / Q000235:genetics*; D013174:Sporotrichosis / Q000453:epidemiology
chemical_list: D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases