Assessment of viability by normal mouse foot-pad and bacillary ATP bioluminescence assay in multibacillary cases treated with an MDT regimen using conventional as well as newer drugs like minocycline and ofloxacin.
The therapeutic effect of a drug regimen of conventional drugs as well as newer drugs like ofloxacin and minocycline in smear-positive multibacillary (MB) leprosy cases was assessed by mouse foot-pad and ATP bioluminiscence methods. Biopsies were taken before starting treatment and after one year of treatment. They were processed for viability assessment by normal mouse foot-pad inoculation and bacillary ATP assay techniques. The test regimen was quite effective in its anti-bacterial effect as it was found to result in loss of bacillary viability in all the cases, as assessed by both methods.
Gupta
U D
UD
Central JALMA Institute for Leprosy, Tajganj, Agra 282 001.
Katoch
K
K
Singh
H B
HB
Natrajan
M
M
Sharma
V D
VD
Katoch
V M
VM
D000255:Adenosine Triphosphate / Q000032:analysis*; D000818:Animals; D005528:Foot / Q000382:microbiology; D006801:Humans; D007917:Leprostatic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D007918:Leprosy / Q000188:drug therapy* / Q000382:microbiology; D008163:Luminescent Measurements; D051379:Mice; D009166:Mycobacterium leprae / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D016896:Treatment Outcome
D007917:Leprostatic Agents; D000255:Adenosine Triphosphate