[Construction and nucleotide sequence assay of human anti-HBs variable region single-chain antibody gene].
The VH and Vk gene which were acquired using phage display technology have been combined by a linker encoding a flexible peptide (Gly4 Ser)3 to form a single-chain. Its nucleotide sequence was assayed after cloning the scFv gene into middle plasmid. The successful construction of single-chain gene lays the foundations for the subsequent expression and production of double functional antibody.
Ren
X
X
Central Research Institution of Infectious Disease, 458th Hospital of PLA, Guangzhou 510602.
Gao
H
H
Su
K
K
Chen
W
W
Yang
A
A
Yu
Z
Z
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D006510:Hepatitis B Antibodies / Q000235:genetics*; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
D006510:Hepatitis B Antibodies; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D007145:Immunoglobulin kappa-Chains