title: Effect of hemoglobin E on glycosylated hemoglobin determinations using different commercial kits.
abstract: The effect of HbE, a hemoglobin variant, on the determination of HbA1/HbA1c using 4 commercial kits based on cation-exchange resin, cation-exchange column chromatography and specific antibody techniques was studied. Fifty-eight normal and 63 HbE heterozygous subjects were tested for HbA1 and HbA1c using 4 commercial kits i.e. Eagles Diagnostics, Boehringer Mannehim (BM), Diastat and Ames DCA 2000. Analyses of the samples by the 4 kits were done within one week and samples were stored at 4 degrees C before analysis. The results showed that HbE affects the determination of glycosylated hemoglobin using cation-exchange based and not kits based on specific antibody techniques.
authors.affiliation: Department of Chemical Pathology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan.
authors.forename: M
authors.initials: M
authors.lastname: Musalmah
authors.forename: J
authors.initials: J
authors.lastname: Normah
authors.forename: W B
authors.initials: WB
authors.lastname: Mohamad
authors.forename: O N
authors.initials: ON
authors.lastname: Salwah
authors.forename: H A
authors.initials: HA
authors.lastname: Fatah
authors.forename: N A
authors.initials: NA
authors.lastname: Zahari
mesh_terms: D000293:Adolescent; D000328:Adult; D002852:Chromatography, Ion Exchange; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis*; D006446:Hemoglobin E / Q000032:analysis*; D006801:Humans; D007158:Immunologic Techniques; D008297:Male; D008875:Middle Aged; D011933:Reagent Kits, Diagnostic
chemical_list: D006442:Glycated Hemoglobin; D006446:Hemoglobin E; D011933:Reagent Kits, Diagnostic