[Features of fibronectin-dependent activation of ribosomal protein S6 kinase (S6K1 and S6K2)].
Integrin family of adhesion receptors play an important role in organizing the actin cytoskeleton and in signal transduction from the extracellular matrix. The previous studies have shown that exposure of fibroblast cells to extracellular matrix proteins activates ribosomal S6 kinase 1 (S6K1) pathway in a ligand dependent manner. Recently, a new, highly homologous ribosomal S6 kinase, termed S6K2, was identified. It has 70% amino acid identity in the overall sequence with S6K1, and the potential phosphorylation sites of S6K1 are conserved in S6K2. However, the N- and C-terminal domains of S6K2 are quite different from those of S6K1. In this study we have examined dynamics of fibronectin-induced activation of these two kinases, transiently expressed in human HEK 293 cells. Differences between profiles of activation of S6K1 and S6K2 were observed in the early period of fibronectin stimulation. Fibronectin-induced changes in S6K2 activity were closely correlated with phosphorylation at Ser423, which is homologues to Ser 434 of S6K1. Although we didn't observe considerable changes in phosphorylation of S6K1 at Ser434, suggesting potential differences in the regulation of these homologous kinases upon fibronectin stimulation.
Val'ovka
T I
TI
Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kyiv, Ukraine.
Filonenko
V V
VV
Velykyï
M M
MM
Drobot
L B
LB
Voterfill
M
M
Matsuka
H Kh
HKh
Hut
I T
IT
D002460:Cell Line; D004789:Enzyme Activation; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D010766:Phosphorylation; D019893:Ribosomal Protein S6 Kinases / Q000378:metabolism*; D015398:Signal Transduction
D005353:Fibronectins; D019893:Ribosomal Protein S6 Kinases
Osoblyvosti fibronektynzalezhnoï aktivatsiï kinaz rybosomnoho bilka S6 (S6K1 i S6K2).