title: Determination of acetaldehyde in tobacco smoke using N-methyl-4-hydrazino-7-nitrobenzofurazan and liquid chromatography/mass spectrometry.
abstract: A liquid chromatography/mass spectrometry (LC/MS) method for the determination of carbonyl compounds based on derivatization with N-methyl-4-hydrazino-7-nitrobenzofurazan (MNBDH) has been developed. Atmospheric pressure chemical ionization (APCI) in the positive mode proved the most versatile ionization technique for MNBD-hydrazones. APCI/MS spectra were recorded and the detection limits were determined for [M+H]+, 13C2 acetaldehyde MNBD-hydrazone has been synthesized and characterized. It is applied as internal standard for the quantification of acetaldehyde. Tobacco smoke has been investigated concerning its carbonyl content. Acetaldehyde was identified as main product and quantified by LC/MS using internal standardization. The result is in good agreement to quantification data obtained with UV/vis detection.
authors.affiliation: Westfälische Wilhelms-Universität Münster, Anorganisch-Chemisches Institut, Abteilung Analytische Chemie, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Zurek
authors.forename: A
authors.initials: A
authors.lastname: Büldt
authors.forename: U
authors.initials: U
authors.lastname: Karst
mesh_terms: D000079:Acetaldehyde / Q000032:analysis* / Q000378:metabolism; D002247:Carbon Isotopes; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006834:Hydrazines / Q000378:metabolism*; D006835:Hydrazones / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D010069:Oxadiazoles / Q000378:metabolism*; D012015:Reference Standards; D012907:Smoking; D014028:Tobacco Smoke Pollution / Q000032:analysis*
chemical_list: D002247:Carbon Isotopes; D006834:Hydrazines; D006835:Hydrazones; C121458:N-methyl-4-hydrazino-7-nitrobenzofurazan; D010069:Oxadiazoles; D014028:Tobacco Smoke Pollution; D000079:Acetaldehyde