Fast enantioseparations of basic analytes by high-performance liquid chromatography using cellulose tris(3,5-dimethylphenylcarbamate)-coated zirconia stationary phases.
In this work, we study the influence of the mobile phase and column temperature on the enantioresolution of basic compounds on microparticulate porous zirconia coated with cellulose tris(3,5-dimethylphenylcarbamate) (CDMPC). The chiral analytes are amino compounds, including a number of beta-blockers. Analytes are eluted with hexane-alcohol mobile phases. We investigated the effect of alcohol (type and concentration), basic eluent additives, and column temperature on the parameters that control resolution (column efficiency, retention and selectivity). Conditions for achieving an adequate separation in the least time have been determined for numerous racemic mixtures. For most solutes, baseline resolution of the enantiomeric pair was achieved in less than 1 min; 12 of 13 pairs were separated in less than 2 min.
Castells
C B
CB
Department of Chemistry, University of Minnesota, Minneapolis 55455, USA.
Carr
P W
PW
D002219:Carbamates / Q000737:chemistry*; D002482:Cellulose / Q000031:analogs & derivatives* / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D048448:Phenylcarbamates; D012997:Solvents; D013237:Stereoisomerism; D013696:Temperature; D015040:Zirconium / Q000737:chemistry*
D002219:Carbamates; D048448:Phenylcarbamates; D012997:Solvents; C065943:cellulose tris-3,5-dimethylphenyl-carbamate; D002482:Cellulose; D015040:Zirconium; C028541:zirconium oxide