Disruption of differentiated functions during viral infection in vivo. V. Mapping of a locus involved in susceptibility of mice to growth hormone deficiency due to persistent lymphocytic choriomeningitis virus infection.
Lymphocytic choriomeningitis virus (LCMV) Armstrong strain selectively and persistently infects the majority of growth hormone (GH) producing cells in the anterior lobe of pituitary glands of C3H/St mice but negligibly infects GH producing cells of BALB/WEHI mice (Oldstone et al., Virology 142, 175--182, 1985; Oldstone et al., Science 218, 1125--1127, 1982). Although infected GH cells remain free of structural damage, disrupted initiation of GH transcription (Klavinskis and Oldstone, J. Gen. Virol. 68, 1867--1873, 1989; Valsamakis et al., Virology 156, 214--220, 1987) occurs with a resultant decrease in the synthesis of GH, leading to a failure of growth and development (Oldstone et al., Science 218, 1125--1127, 1982). Microsatellite mapping of DNA obtained from 101 individual C3H/St x BALB/WEHI F1 x F1 mice shows that the growth failure correlates with host genes linked (P value 0.0008) on chromosome 17 just outside of the H-2D MHC site between D17 Mit24 and D17 Mit51, a distance of 2.5 cM. The genetic mapping done here excludes alpha-dystroglycan (alpha-DG), a known receptor for LCMV (Cao et al., Science 282, 2079--2081, 1998) in pathogenesis of GH disease, as alpha-DG is encoded in the mouse by a gene residing on chromosome 9 (Yotsumoto et al., Hum. Mol. Genet. 5, 1259--1267, 1996).
Bureau
J F
JF
Unite des Virus Lents, URA 1930 CNRS, Institut Pasteur, 75724 Paris Cedex 15, France.
Le Goff
S
S
Thomas
D
D
Parlow
A F
AF
de la Torre
J C
JC
Homann
D
D
Brahic
M
M
Oldstone
M B
MB
D000818:Animals; D001835:Body Weight; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D003598:Cytoskeletal Proteins / Q000235:genetics; D004393:Dwarfism, Pituitary / Q000150:complications* / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D049030:Dystroglycans; D005260:Female; D008040:Genetic Linkage / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D013006:Growth Hormone / Q000032:analysis / Q000172:deficiency*; D008126:Lod Score; D008216:Lymphocytic Choriomeningitis / Q000150:complications* / Q000503:physiopathology / Q000821:virology; D008217:Lymphocytic choriomeningitis virus / Q000502:physiology*; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D018895:Microsatellite Repeats / Q000235:genetics; D010902:Pituitary Gland / Q000737:chemistry; D013223:Statistics as Topic; D013577:Syndrome
D003598:Cytoskeletal Proteins; D008562:Membrane Glycoproteins; D049030:Dystroglycans; D013006:Growth Hormone