title: Fine-mapping and construction of a bovine contig spanning the ovine callipyge locus.
abstract: The callipyge (CLPG) gene was fine-mapped by linkage analysis to a 4.6-cM chromosome interval on distal ovine OAR18q, flanked by microsatellite markers IDVGA30 and OY3. The OAR18q linkage map and human HSA14q transcript map were aligned by genotyping two bovine-hamster whole-genome radiation hybrid panels with the microsatellite markers, as well as with sequences corresponding to HSA 14q genes. Using Type I loci mapping to the IDVGA30-OY3 interval as anchor points, we have constructed a 1.4-Mb bovine BAC contig containing the IDVGA30-OY3 interval. We demonstrate that the IDVGA30-OY3 interval spans approximately 770 kb and contains at least four genes: YY1, WARS, DLK1, and GTL2.
authors.affiliation: Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan 84322-4700, USA.
authors.forename: T L
authors.initials: TL
authors.lastname: Shay
authors.forename: S
authors.initials: S
authors.lastname: Berghmans
authors.forename: K
authors.initials: K
authors.lastname: Segers
authors.forename: S
authors.initials: S
authors.lastname: Meyers
authors.forename: J E
authors.initials: JE
authors.lastname: Beever
authors.forename: J E
authors.initials: JE
authors.lastname: Womack
authors.forename: M
authors.initials: M
authors.lastname: Georges
authors.forename: C
authors.initials: C
authors.lastname: Charlier
authors.forename: N E
authors.initials: NE
authors.lastname: Cockett
mesh_terms: D000818:Animals; D002417:Cattle; D003001:Cloning, Molecular; D020451:Contig Mapping; D006224:Cricetinae; D003433:Crosses, Genetic; D005838:Genotype; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D024510:Muscle Development; D018656:Muscle Fibers, Fast-Twitch / Q000378:metabolism; D018482:Muscle, Skeletal / Q000254:growth & development / Q000378:metabolism; D011506:Proteins / Q000235:genetics*; D021101:Radiation Hybrid Mapping; D012756:Sheep
chemical_list: D011506:Proteins