title: The effects of melatonin in glutamate-induced neurotoxicity of rat cerebellar granular cell culture.
abstract: In the present study, melatonin was tested in subsequent doses in glutamate induced neurotoxicity in cerebellar granular cell culture of rat pups. Glutamate at 10(-7) M was found to induce neuronal cell death. The dead cell score was 2.75+/-0.7 in the control, while it was found to be 35.12+/-1.8 in the glutamate-administered group (P<0.0001). Melatonin very potently blocked glutamate neurotoxicity at all doses tested, with 10(-3) M, the highest dose tested, being the most effective. Glutamate may exert a neuroprotective effect by blocking one or more steps of the oxidation cascade in neurons and this effect may be blocked by melatonin.
authors.affiliation: Department of Pharmacology, Atatürk University, Medical Faculty, Erzurum, Turkey. akcahan@atauni.edu.tr
authors.forename: A
authors.initials: A
authors.lastname: Gepdiremen
authors.forename: S
authors.initials: S
authors.lastname: Düzenli
authors.forename: A
authors.initials: A
authors.lastname: Hacimüftüoglu
authors.forename: D
authors.initials: D
authors.lastname: Bulucu
authors.forename: H
authors.initials: H
authors.lastname: Süleyman
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000975:Antioxidants / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D018698:Glutamic Acid / Q000494:pharmacology*; D008550:Melatonin / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000975:Antioxidants; D018698:Glutamic Acid; D008550:Melatonin