Evaluation of iron status in anemic patients with rheumatoid arthritis using an automated immunoturbidimetric assay for transferrin receptor.
We have evaluated a newly introduced immunoturbidimetric transferrin receptor assay (IdeA TfR-IT, Orion Diagnostica, Finland) in healthy subjects and in a study population consisting of patients with rheumatoid arthritis and juvenile chronic arthritis. The IdeA TfR-IT assay was found to provide reproducible results which were in good agreement with the ELISA assays from Orion Diagnostica (IDeA-ELISA, correlation R2=0.8, n=102) and R&D systems (Quantikine TfR ELISA assay, correlation R2=0.95, n=39). The analysis of the patient samples suggested that, on the basis of serum transferrin receptor and ferritin concentrations, in approximately one third of patients with rheumatoid arthritis anemia is due to the depletion of iron stores. Apparently, in all patients with rheumatoid arthritis iron deficiency must be considered as a potential cause of the anemia. Now, that assays which are suitable for automated analyzers have become available for the measurement of serum transferrin receptor, this analyte has the potential to become a part of the routine evaluation of iron status.
Punnonen
K
K
Department of Clinical Chemistry, Kuopio University Hospital, Finland. kari.punnonen@kuh.fi
Kaipiainen-Seppänen
O
O
Riittinen
L
L
Tuomisto
T
T
Hongisto
T
T
Penttilä
L
L
D000293:Adolescent; D000328:Adult; D000740:Anemia / Q000097:blood*; D001171:Arthritis, Juvenile / Q000097:blood; D001172:Arthritis, Rheumatoid / Q000097:blood*; D001331:Automation; D002648:Child; D002675:Child, Preschool; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005293:Ferritins / Q000494:pharmacology; D006801:Humans; D007223:Infant; D007501:Iron / Q000378:metabolism*; D008297:Male; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D011990:Receptors, Transferrin / Q000097:blood*; D012016:Reference Values; D015203:Reproducibility of Results
D011990:Receptors, Transferrin; D005293:Ferritins; D007501:Iron