Investigation of avenacin-deficient mutants of Avena strigosa.
The biosynthesis of cyclic triterpenoids in ten saponin-deficient (sad) mutant varieties of the diploid oat Avena strigosa is reported. Two mutants were found to be deficient in 2,3-oxidosqualene:beta-amyrin cyclase (OSbetaAC) (EC 5.4.99) and thus unable to produce the beta-amyrin necessary for the production of avenacins. The other mutants studied had post beta-amyrin lesions. 2,3-Oxidosqualene:cycloartenol cyclase (OSCC) (EC 5.4.99.8) needed for sterol formation was present in all ten mutants.
Trojanowska
M R
MR
Department of Biological Sciences, University of Hull, UK. miranda.trojanowska@bbsrc.ac.uk
Osbourn
A E
AE
Daniels
M J
MJ
Threlfall
D R
DR
D018554:Avena / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics; D002250:Carbon Radioisotopes; D002851:Chromatography, High Pressure Liquid; D019751:Intramolecular Transferases / Q000378:metabolism; D008798:Mevalonic Acid / Q000737:chemistry; D009154:Mutation; D012503:Saponins / Q000235:genetics*
D002250:Carbon Radioisotopes; D012503:Saponins; C091460:avenacin A 1; D019751:Intramolecular Transferases; C039924:2,3-oxidosqualene-beta-amyrin-cyclase; C021923:cycloartenol synthase; D008798:Mevalonic Acid