title: Polymorphism in the regulatory sequence of the human hsp70-1 gene does not affect heat shock factor binding or heat shock protein synthesis.
abstract: A bi-allelic polymorphism found in the regulatory region of the human heat shock (HS) protein (HSP) hsp70-1 gene, which comprises an A-->C transversion, 3 bp upstream of the HS element (HSE), has been associated with extended HLA haplotypes. In view of the chaperoning and protective functions of Hsp70, we investigated whether this hsp70-1 bi-allelic polymorphism could modulate the stress response, which may relate to enhanced resistance or susceptibility to certain diseases. We compared the basal and HS-induced HS factor (HSF)-binding activity of the two polymorphic HSEs, hsp70-1 mRNA accumulation and HSP expression in two human Epstein Barr virus (EBV)-transformed B cell lines typed for hsp70-1 promoter alleles. Our results suggest that hsp70-1 promoter polymorphism does not influence HSF-binding activity, hsp70 mRNA accumulation or synthesis in human EBV-transformed B cell lines.
authors.affiliation: Laboratoire de Biologie, Physiologie et Pathologie de la Respiration, UFR Cochin Port-Royal, Saint-Jacques, Université René Descartes, Paris, France. Florence.Favatier@cochin.univ-paris5.fr
authors.forename: F
authors.initials: F
authors.lastname: Favatier
authors.forename: M R
authors.initials: MR
authors.lastname: Jacquier-Sarlin
authors.forename: E
authors.initials: E
authors.lastname: Swierczewski
authors.forename: B S
authors.initials: BS
authors.lastname: Polla
mesh_terms: D000483:Alleles; D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism; D001483:Base Sequence; D001667:Binding, Competitive; D002461:Cell Line, Transformed; D017124:Conserved Sequence / Q000235:genetics; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D006680:HLA Antigens / Q000235:genetics; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D006239:Haplotypes; D000076249:Heat Shock Transcription Factors; D018869:Heat-Shock Response / Q000235:genetics*; D006801:Humans; D011110:Polymorphism, Genetic / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020218:Response Elements / Q000235:genetics*; D014157:Transcription Factors; D020298:U937 Cells
chemical_list: D004268:DNA-Binding Proteins; D006680:HLA Antigens; D018840:HSP70 Heat-Shock Proteins; D000076249:Heat Shock Transcription Factors; D012333:RNA, Messenger; D014157:Transcription Factors; D004247:DNA