title: Influence of albumin and collagen on the cell mortality evoked by zinc oxide-eugenol in vitro.
abstract: Zinc oxide-eugenol (ZOE) is cytotoxic in vitro but does not cause pulp damage. Various dentin proteins may play a protective role in vivo. We investigated the influence of bovine serum albumin (BSA) and collagen on ZOE cytotoxicity in the Millipore filter test and a dentin barrier test. Agar or agarose with 1% or 5% BSA or calf collagen (1% and 2%) were introduced between L-929 fibroblasts and test specimens. ZOE was severely toxic in the absence of BSA. However BSA (1% and 3%) reduced cell damage to 50% and 35%. In contrast, collagen did not provide protection from ZOE cytotoxicity. When dentin was loaded with 3% BSA and the "pulpal" side of a dentin barrier test chamber was perfused with cell culture medium containing 3% BSA, cytotoxicity of ZOE was completely inhibited. Therefore we conclude that albumin may be one factor responsible for the absence of ZOE toxicity in pulp studies.
authors.affiliation: Clinic of Operative Dentistry and Periodontology, University of Regensburg, Regensburg, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Schmalz
authors.forename: M
authors.initials: M
authors.lastname: Hoffmann
authors.forename: K
authors.initials: K
authors.lastname: Weis
authors.forename: H
authors.initials: H
authors.lastname: Schweikl
mesh_terms: D000362:Agar; D000818:Animals; D002417:Cattle; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology*; D003470:Culture Media; D019610:Cytoprotection; D003804:Dentin / Q000502:physiology; D015194:Diffusion Chambers, Culture; D005347:Fibroblasts / Q000187:drug effects; D051379:Mice; D008851:Micropore Filters; D020011:Protective Agents / Q000494:pharmacology*; D012685:Sepharose; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D015035:Zinc Oxide-Eugenol Cement / Q000633:toxicity*
chemical_list: D003470:Culture Media; D020011:Protective Agents; D015035:Zinc Oxide-Eugenol Cement; D012710:Serum Albumin, Bovine; D000362:Agar; D003094:Collagen; D012685:Sepharose