Effect of hydrogen peroxide on guinea pig nasal mucosa vasculature.
The effect of hydrogen peroxide (H2O2) on guinea pig nasal mucosa vasculature was studied by in vitro assay. H2O2 elicited relaxation of guinea pig nasal mucosa strips precontracted with phenylephrine in a concentration-dependent manner. The relaxant response to H2O2 was abolished in the presence of catalase. Preincubation of the strips with N(G)-nitro-L-arginine methyl ester or methylene blue significantly attenuated the relaxant responses elicited by H2O2. Fluorescence caused by DAF-2 DA, a fluorescence indicator for nitric oxide, was observed along the nasal mucosa vasculature in response to H2O2. These results suggest that H2O2 induced relaxation of the guinea pig nasal mucosa vasculature and that this relaxation is mediated by the NO/cGMP pathway.
Hirai
T
T
Department of Otorhinolaryngology, Hiroshima University School of Medicine, Japan.
Tsuru
H
H
Tanimitsu
N
N
Takumida
M
M
Watanabe
H
H
Yajin
K
K
Sasa
M
M
D000818:Animals; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006168:Guinea Pigs; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008297:Male; D008751:Methylene Blue / Q000494:pharmacology; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009297:Nasal Mucosa / Q000098:blood supply* / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism*; D016877:Oxidants / Q000494:pharmacology*; D014664:Vasodilation / Q000187:drug effects* / Q000502:physiology
D004791:Enzyme Inhibitors; D016877:Oxidants; D009569:Nitric Oxide; D006861:Hydrogen Peroxide; D008751:Methylene Blue; D019331:NG-Nitroarginine Methyl Ester