title: Frequent cytolytic T-cell responses to peptide MAGE-A10(254-262) in melanoma.
abstract: MAGE genes encode tumor-specific shared antigens that are among the most interesting candidates for cancer vaccines. Despite extensive studies, however, CD8+ T-cell responses to MAGE-derived epitopes have been detected only occasionally in cancer patients, even after vaccination. In contrast with these findings, we report here that HLA-A2 melanoma patients respond frequently to the recently identified peptide MAGE-A10(254-262). Indeed, as assessed by staining with fluorescent HLA-A2/peptide MAGE-A10(254-262) tetramers, CD8+ T cells directed against this peptide were readily detectable in a large proportion of HLA-A2+ melanoma patients. These results provide new insight into the immunogenicity of MAGE antigens and underline the potential usefulness of MAGE-A10 peptide-based cancer vaccines.
authors.affiliation: Division of Clinical Onco-Immunology, Ludwig Institute for Cancer Research, University Hospital, Lausanne, Switzerland.
authors.forename: D
authors.initials: D
authors.lastname: Valmori
authors.forename: V
authors.initials: V
authors.lastname: Dutoit
authors.forename: V
authors.initials: V
authors.lastname: Rubio-Godoy
authors.forename: C
authors.initials: C
authors.lastname: Chambaz
authors.forename: D
authors.initials: D
authors.lastname: Liénard
authors.forename: P
authors.initials: P
authors.lastname: Guillaume
authors.forename: P
authors.initials: P
authors.lastname: Romero
authors.forename: J C
authors.initials: JC
authors.lastname: Cerottini
authors.forename: D
authors.initials: D
authors.lastname: Rimoldi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000951:Antigens, Neoplasm; D019556:COS Cells; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D004274:DNA, Recombinant; D004305:Dose-Response Relationship, Drug; D015789:HLA-A2 Antigen / Q000235:genetics / Q000276:immunology; D006801:Humans; D008545:Melanoma / Q000276:immunology*; D009363:Neoplasm Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D009842:Oligopeptides / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology; D010957:Plasmids / Q000235:genetics; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D004274:DNA, Recombinant; D015789:HLA-A2 Antigen; C119317:MAGE-A10 antigen; D009363:Neoplasm Proteins; D009842:Oligopeptides; D014409:Tumor Necrosis Factor-alpha