Tributyrin specifically induces a lipase with a preference for the sn-2 position of triglyceride in Geotrichum sp. FO401B.
The extracellular Lipases A and C produced by Geotrichum sp. FO401B have a preference for the sn-1,3 and sn-2 positions of triglyceride, respectively. Total production of these lipases was increased by plant oils and tributyrin. Butyl Toyopearl column chromatography demonstrated that only Lipase C was produced in the presence of tributyrin. Lipase C hydrolysed natural fats except sardine oil preferentially at the sn-2 position, but it showed little stereoselectivity for triolein.
Ota
Y
Y
Department of Applied Biochemistry, Faculty of Applied Biological Science, Hiroshima University, Higashi-Hiroshima, Japan. yasota@hiroshima-u.ac.jp
Sawamoto
T
T
Hasuo
M
M
D004790:Enzyme Induction; D005848:Geotrichum / Q000187:drug effects* / Q000201:enzymology; D008049:Lipase / Q000096:biosynthesis / Q000378:metabolism*; D013379:Substrate Specificity; D014280:Triglycerides / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*
D014280:Triglycerides; D008049:Lipase; C005830:tributyrin