title: Genomic binding sites of the yeast cell-cycle transcription factors SBF and MBF.
abstract: Proteins interact with genomic DNA to bring the genome to life; and these interactions also define many functional features of the genome. SBF and MBF are sequence-specific transcription factors that activate gene expression during the G1/S transition of the cell cycle in yeast. SBF is a heterodimer of Swi4 and Swi6, and MBF is a heterodimer of Mbpl and Swi6 (refs 1, 3). The related Swi4 and Mbp1 proteins are the DNA-binding components of the respective factors, and Swi6 mayhave a regulatory function. A small number of SBF and MBF target genes have been identified. Here we define the genomic binding sites of the SBF and MBF transcription factors in vivo, by using DNA microarrays. In addition to the previously characterized targets, we have identified about 200 new putative targets. Our results support the hypothesis that SBF activated genes are predominantly involved in budding, and in membrane and cell-wall biosynthesis, whereas DNA replication and repair are the dominant functions among MBF activated genes. The functional specialization of these factors may provide a mechanism for independent regulation of distinct molecular processes that normally occur in synchrony during the mitotic cell cycle.
authors.affiliation: Department of Biochemistry, Stanford University Medical Center, California 94305, USA.
authors.forename: V R
authors.initials: VR
authors.lastname: Iyer
authors.forename: C E
authors.initials: CE
authors.lastname: Horak
authors.forename: C S
authors.initials: CS
authors.lastname: Scafe
authors.forename: D
authors.initials: D
authors.lastname: Botstein
authors.forename: M
authors.initials: M
authors.lastname: Snyder
authors.forename: P O
authors.initials: PO
authors.lastname: Brown
mesh_terms: D001665:Binding Sites; D002453:Cell Cycle; D004271:DNA, Fungal / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D020411:Oligonucleotide Array Sequence Analysis; D011233:Precipitin Tests; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; C083209:MBP1 protein, S cerevisiae; C081540:SBF protein, S cerevisiae; C075111:SWI6 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors