Systematic analysis of oxidative degradation of polysaccharides using PAGE and HPLC--MS.
Oxidation of polysaccharides yields hydroxyaldehydes and hydroxycarboxylic acids. Aldehydes and carboxylic acids were separately conjugated to 8-aminonaphthalene-1,3,6-trisulfonic acid (ANTS) or tyrosine t-butyl ester (TBT). The ANTS-labeled derivatives were separated by molecular size on PAGE gels and detected by fluorescence. TBT-labeled derivatives were separated by reverse phase chromatography on a C18-HPLC column and analyzed by positive ion electrospray mass spectroscopy (HPLC--MS). This combination of procedures allowed a systematic analysis of carbohydrate oxidation products.
Ovalle
R
R
Department of Biology, Center for the Study of Gene Structure and Function, Hunter College of CUNY, New York, NY 10021, USA.
Soll
C E
CE
Lim
F
F
Flanagan
C
C
Rotunda
T
T
Lipke
P N
PN
D000447:Aldehydes / Q000737:chemistry / Q000378:metabolism; D002264:Carboxylic Acids / Q000737:chemistry / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006861:Hydrogen Peroxide / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D010084:Oxidation-Reduction; D011134:Polysaccharides / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013481:Superoxides
D000447:Aldehydes; D002264:Carboxylic Acids; C045076:Fenton's reagent; D011134:Polysaccharides; D013481:Superoxides; D006861:Hydrogen Peroxide; D007501:Iron