Identification of novel genes differentially expressed in PMA-induced HL-60 cells using cDNA microarrays.
Identification of normal growth and differentiation-inducing proteins and their interaction in normal development have made it possible to elucidate the molecular basis of normal development and the mechanisms uncoupling growth and differentiation during tumor development. The development of cancer and the experimental reversal of tumorigenicity are accompanied by complex changes in patterns of gene expression. cDNA microarrays provide a powerful tool for studying these phenomena. In the present study, a high-density microarray of human cDNA elements was used to search for differences in gene expression associated with differentiation of human promyelic leukemia HL-60 cells. Microarrays containing 3,063 human cDNAs were printed on glass slides with high-speed robotics. These DNA 'chips' were used to quantitatively monitor differential expression of the cognate human genes using a highly sensitive two-color hybridization assay. The identification of known and novel phorbol ester-regulated genes in hematopoietic progenitor cells demonstrates the sensitivity of the assay.
Seo
J
J
Department of Immunology, Kyungpook National University School of Medicine, Deagu, Korea.
Kim
M
M
Kim
J
J
D001483:Base Sequence; D002273:Carcinogens / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D020869:Gene Expression Profiling; D018922:HL-60 Cells / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D006412:Hematopoietic Stem Cells / Q000378:metabolism; D006801:Humans; D007951:Leukemia, Myeloid / Q000378:metabolism / Q000473:pathology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020543:Proteome / Q000032:analysis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D002273:Carcinogens; D020543:Proteome; D013755:Tetradecanoylphorbol Acetate