title: The role of the Golgi apparatus during terminal differentiation of mouse urothelial surface cells.
abstract: The development of the Golgi apparatus in the surface cells of mouse urinary bladder during embryonic development was investigated by electronmicroscopic cytochemistry. The distributions of NADPase and TPPase activities were studied in the urinary bladder during day 15 to day 18 of gestation. At the early embryonic stage, the products of the NADPase and TPPase reactions were visible exclusively in 1 to 2 medial and/or trans Golgi saccules. The strongest increment of NADPase and TPPase positive Golgi cisternae was detected at day 17 when the activity of the urothelial cells was very prominent. At this age, NADPase activity was detected also in lysosomes and on the apical surface of the urothelial cells. The highest distribution pattern of NADPase and TPPase activities observed at this stage rapidly decreases at day 18 of fetal life. The results suggest that the organization of the Golgi apparatus reflected the intensity of the processes occuring in the urothelial cells during gestation.
authors.affiliation: Institute of Cell Biology, Medical Faculty, Ljubljana, Slovenia. Psenicnik@IBMI.MF.UNI-LJ.SI
authors.forename: M
authors.initials: M
authors.lastname: Psenicnik
authors.forename: K
authors.initials: K
authors.lastname: Jezernik
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D005314:Embryonic and Fetal Development; D005260:Female; D006056:Golgi Apparatus / Q000502:physiology* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D009708:Nucleotidases / Q000378:metabolism; D013834:Thiamine Pyrophosphatase / Q000378:metabolism; D001743:Urinary Bladder / Q000196:embryology* / Q000378:metabolism / Q000648:ultrastructure; D019459:Urothelium / Q000166:cytology
chemical_list: C021896:2'-nucleotidase; D009708:Nucleotidases; D013834:Thiamine Pyrophosphatase