Immunofluorescence analysis of T-cell responses in health and disease.
The use of flow cytometry to study the functional responses of T cells by immunofluorescent staining for intracellular cytokines and other markers is a growing field of clinical interest. In this article, we describe methods for the rapid evaluation of T-cell responses to mitogens and specific antigens and explore how these assays might be valuable in various clinical settings.
Maecker
H T
HT
BD Immunocytometry Systems, San Jose, California 95131, USA.
Maino
V C
VC
Picker
L J
LJ
D001327:Autoimmune Diseases / Q000276:immunology; D016207:Cytokines / Q000032:analysis; D005434:Flow Cytometry / Q000379:methods*; D005455:Fluorescent Antibody Technique / Q000379:methods*; D006678:HIV / Q000276:immunology; D006801:Humans; D007154:Immune System Diseases / Q000276:immunology; D008213:Lymphocyte Activation; D016377:Organ Transplantation; D012680:Sensitivity and Specificity; D013601:T-Lymphocytes / Q000276:immunology*; D014612:Vaccines / Q000276:immunology
D016207:Cytokines; D014612:Vaccines