Genetic screening for progressive retinal atrophy in the Australian population of Irish Setters.
OBJECTIVE
To determine whether the rcd-1 mutation causing progressive retinal atrophy (PRA) in Irish Setters is in the Australian breeding population.


METHODS
DNA samples were tested for the mutation using the Polymerase Chain Reaction and specific primer nucleotides to amplify the phosphodiesterase gene followed by restriction enzyme cleavage and fragment size determination.


RESULTS
No mutant alleles were found in 38 Irish Setters, representing over 80% of all major breeding stock in five Australian states.


CONCLUSIONS
It is likely that the Australian population of Irish Setters is free of the rcd-1 form of PRA.
Maroudas
P
P
National Vision Research Institute of Australia, 386 Cardigan Street, Carlton, Victoria 3053.
Jobling
A I
AI
Augusteyn
R C
RC
D000818:Animals; D001315:Australia / Q000453:epidemiology; D017931:DNA Primers; D004283:Dog Diseases / Q000453:epidemiology* / Q000235:genetics*; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D008403:Mass Screening / Q000662:veterinary; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012174:Retinitis Pigmentosa / Q000453:epidemiology / Q000235:genetics / Q000662:veterinary*
D017931:DNA Primers