title: Dextran reduces surfactant inhibition by meconium.
abstract: We have previously shown that an addition of dextran to surfactant increases resistance to inactivation by albumin. The present study was designed to investigate whether dextran has a protective effect against inactivation of surfactant by meconium. The dynamic surface properties of various mixtures of modified natural surfactant (Curosurf), meconium and dextran were evaluated with the pulsating bubble technique. In the absence of meconium, an addition of dextran at 10 mg ml(-1) significantly shortened the surface adsorption time of Curosurf suspended in normal saline at a concentration of 5 mg ml(-1) from 1.6+/-1.3 (mean +/- SD) sec to 0.4+/-0.4 sec (p < 0.05). Meconium at 5 mg ml(-1) increased the mean minimum surface tension (gammamin) after 3 min of pulsation from 2.1 mN m(-1) to 24 mN m(-1) (p < 0.05). An addition of dextran at 5 and 10 mg ml(-1) to the meconium-surfactant mixture significantly reduced the gammamin to 6.4+/-4.5 mN m(-1) and 2.6+/-5.6 mN m(-1), respectively (p < 0.05 vs dextran 0 mg ml(-1)). Meconium also increased the maximum surface tension (gammamax) of Curosurf from about 33 mN m(-1) to 53 mN m(-1). An addition of dextran at 5 and 10 mg ml(-1) to the meconium-surfactant mixture reduced the gammamax to 41 and 39 mN m(-1), respectively (p < 0.05 vs dextran 0 mg ml(-1)). There were significant negative correlations between dextran concentration and both gammamin and gammamax. We conclude that dextran reduces surfactant inhibition by meconium in a dose-dependent manner.
authors.affiliation: Department of Woman and Child Health, Karolinska Hospital, Stockholm, Sweden.
authors.forename: K
authors.initials: K
authors.lastname: Tashiro
authors.forename: T
authors.initials: T
authors.lastname: Kobayashi
authors.forename: B
authors.initials: B
authors.lastname: Robertson
mesh_terms: D000327:Adsorption; D000704:Analysis of Variance; D000818:Animals; D001688:Biological Products; D003911:Dextrans / Q000627:therapeutic use*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007231:Infant, Newborn; D008470:Meconium / Q000187:drug effects*; D010743:Phospholipids; D011663:Pulmonary Surfactants / Q000187:drug effects* / Q000494:pharmacology*; D013500:Surface Tension; D013552:Swine
chemical_list: D001688:Biological Products; D003911:Dextrans; D010743:Phospholipids; D011663:Pulmonary Surfactants; C068291:poractant alfa