cDNA cloning of the Cry1Aa receptor variants from Bombyx mori and their expression in mammalian cells.
We cloned cDNA of three variants of BtR175, a putative Bombyx mori receptor for Bacillus thuringiensis Cry1Aa delta-endotoxin by PCR. These variants were likely to be allelic to BtR175. cDNA of BtR175b, the most distant variant from BtR175, was introduced into mammalian cells. BtR175b protein was expressed in the plasma membrane of the cells and showed binding activity to Cry1Aa.
Ikawa
S
S
Department of Applied Biochemistry, Osaka Prefecture University, Sakai, Japan.
Tsuda
Y
Y
Fukada
T
T
Sugimoto
K
K
Himeno
M
M
D017398:Alternative Splicing; D019943:Amino Acid Substitution; D000818:Animals; D001426:Bacterial Proteins; D012831:Bombyx / Q000235:genetics*; D019556:COS Cells; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary; D014644:Genetic Variation; D006801:Humans; D019476:Insect Proteins; D008322:Mammals; D008969:Molecular Sequence Data; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; C095416:Cry toxin receptors; D018076:DNA, Complementary; D019476:Insect Proteins; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins