title: Immunization of cross-bred cattle against Hyalomma anatolicum anatolicum by purified antigens.
abstract: Extracts prepared from unfed larvae of Hyalomma anatolicum anatolicum were purified by immunoaffinity chromatography using anti-gut IgG as ligand. Affinity purified antigen (Aff-GHLAg) was used to immunize cross-bred (Bos taurus x B. indicus) calves of 6-7 months of age. Immunized calves rejected 70.6% larvae, 54.5% nymphs and 61.9% adults. No significant changes in the engorged weight of females was observed; however, significant decrease in the engorgement weight of larvae and nymphs was recorded. There was a significant decrease in the emerging nymphs (p < 0.05) and adults (p < 0.01) of the tick stages fed on immunized animals. SDS-PAGE analysis revealed three antigenic proteins of 100, 59.4 and 37 kDa responsible for induction of resistance in the host.
authors.affiliation: Entomology Laboratory, Division of Parasitology, Indian Veterinary Research Institute, Uttar Pradesh.
authors.forename: G
authors.initials: G
authors.lastname: Das
authors.forename: S
authors.initials: S
authors.lastname: Ghosh
authors.forename: M H
authors.initials: MH
authors.lastname: Khan
authors.forename: J K
authors.initials: JK
authors.lastname: Sharma
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology / Q000302:isolation & purification; D001947:Breeding; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology / Q000517:prevention & control*; D002846:Chromatography, Affinity / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005247:Feeding Behavior; D009758:Nymph / Q000502:physiology; D011817:Rabbits; D012098:Reproduction; D012967:Sodium Dodecyl Sulfate; D013984:Tick Infestations / Q000517:prevention & control / Q000662:veterinary*; D013987:Ticks / Q000276:immunology* / Q000502:physiology; D014611:Vaccination / Q000379:methods
chemical_list: D000941:Antigens; D012967:Sodium Dodecyl Sulfate