Antibacterial action of tryptanthrin and kaempferol, isolated from the indigo plant (Polygonum tinctorium Lour.), against Helicobacter pylori-infected Mongolian gerbils.
We evaluated the effect of tryptanthrin and kaempferol, both isolated from Polygonum tinctorium Lour., against Helicobacter pylori colony formation in vitro and in H. pylori-infected Mongolian gerbils. H. pylori suspension was mixed with solution of tryptanthrin and/or kaempferol and placed onto agar plates. These plates were incubated at 37 degrees C, under 10% CO2 for 5 days, and the H. pylori colonies were counted. For the in vivo experiment, Mongolian gerbils were inoculated with H. pylori ATCC 43504 orally. After 4 weeks, the infected gerbils were given tryptanthrin and/or kaempferol, administered orally, twice a day for 10 days. The animals were killed and the number of live H. pylori in their stomachs was determined. In vitro both tryptanthrin and kaempferol significantly decreased the numbers of H. pylori colonies a dose-dependent manner. An additive effect on colony formation was observed with the combined use. In the in vivo experiment, oral administration of tryptanthrin and/or kaempferol significantly decreased the numbers of colonies in the gerbils' stomachs. We concluded that tryptanthrin and kaempferol were effective against H. pylori in vivo.
Kataoka
M
M
Fujisaki Institute, Hayashibara Biochemical Laboratories, Okayama, Japan.
Hirata
K
K
Kunikata
T
T
Ushio
S
S
Iwaki
K
K
Ohashi
K
K
Ikeda
M
M
Kurimoto
M
M
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005419:Flavonoids; D005849:Gerbillinae; D016481:Helicobacter Infections / Q000188:drug therapy*; D016480:Helicobacter pylori; D044949:Kaempferols; D008297:Male; D019664:Polygonaceae; D011794:Quercetin / Q000031:analogs & derivatives / Q000627:therapeutic use*; D011799:Quinazolines / Q000627:therapeutic use*
D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D044949:Kaempferols; D011799:Quinazolines; C046243:tryptanthrine; C006552:kaempferol; D011794:Quercetin