Inhibition of the antibody responses to rat blood transfusion antigens in mice by boar seminal immunosuppressive fraction.
OBJECTIVE
Immunosuppressive fraction of boar seminal vesicle fluid (ISF) was tested to muffle primary and secondary antibody responses to xenotranfusions. Contemporaneously, heparin non-binding fraction of seminal plasma (H- fraction), presumed to be identical to ISF, was used to support the results.


METHODS
To study their similarity, ISF and H- fraction were analyzed by high-performance liquid chromatography and the separated proteins by N-terminal sequencing. In sera of mice treated with ISF or H- fraction, the productions of antibodies against rat erythrocytes and blood serum were evaluated by enzyme-linked immunosorbent assay (ELISA). The productions of IgM, IgA, and IgG subclasses were followed by sandwich ELISA.


RESULTS
ISF and H- fraction were proved to be equal complexes of porcine seminal plasma (PSP) proteins PSP I and PSP II. Both inhibited antibody responses to rat erythrocytes and serum and the concentrations of IgM, IgG, IgG1 and IgG2 after the first transfusion with a long-lasting effect. Both suppressed the secondary antibody production if applied before the second transfusion. IgA and IgG3 stayed uninfluenced. ISF and H- fraction had an equal immunosuppressive effect.


CONCLUSIONS
ISF was characterized biochemically, found to be identical to H- fraction, and determined to be powerful in overcoming unwanted exaggerated antibody responses to xenotransfusion.
Veselský
L
L
Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague. veselsky@img.cas.cz
Jonáková
V
V
Dostál
J
J
Holán
V
V
Voburka
Z
Z
Drahorád
J
J
Manásková
P
P
Zelezná
B
B
D000818:Animals; D000910:Antibodies, Heterophile / Q000097:blood*; D015478:Antigens, Heterophile / Q000276:immunology*; D001769:Blood / Q000276:immunology*; D001803:Blood Transfusion; D005260:Female; D007114:Immunization; D007132:Immunoglobulin Isotypes / Q000097:blood; D007166:Immunosuppressive Agents / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011506:Proteins / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012661:Semen / Q000276:immunology*; D029589:Seminal Plasma Proteins; D013552:Swine
D000910:Antibodies, Heterophile; D015478:Antigens, Heterophile; D007132:Immunoglobulin Isotypes; D007166:Immunosuppressive Agents; D011506:Proteins; D029589:Seminal Plasma Proteins