Polysaccharides of green Arabica and Robusta coffee beans.
Two independent procedures for the quantitative determination of the polysaccharide content of Arabica Caturra (Coffea arabica var. Caturra) and Robusta ROM (Coffea canephora var. ROM) green coffee beans showed that they both contained identical amounts of polysaccharide. Cell wall material (CWM) was prepared from the beans and partial solubilisation of component polysaccharides was effected by sequential extraction with water, 1 M KOH, 0.3% NaClO2, 4 M KOH and 8 M KOH. The monosaccharide compositions of the CWMs were similar, although Arabica beans contained slightly more mannose than Robusta. In the latter, more arabinogalactan was solubilised during preparation of the CWM and the water-soluble fraction of the CWM contained higher amounts of galactomannan than in Arabica. Linkage analysis indicated that the galactomannans possessed unbranched to branched mannose ratios between 14:1 and 30:1 which is higher than previously reported. No major difference in the structural features of the galactomannans between species was found. The arabinogalactans were heterogeneous both with regard to the degree of branching and the degree of polymerisation of their arabinan side-chains. Compared to Arabica, Robusta appeared to contain greater amounts of arabinogalactans with longer side chains. It is concluded that there was no detectable difference between the Arabica and Robusta varieties of this study in their absolute polysaccharide content or in the gross structural features of their galactomannans. Differences were apparent both in the structural features and ease of solubility of the arabinogalactans but a more detailed study of several varieties of Arabica and Robusta will be required to determine whether these differences occur consistently between species.
Fischer
M
M
Nestle Research Center, Vers-Chez-les-Blancs, Lausanne, Switzerland. monica.fischer@rdls.nestle.com
Reimann
S
S
Trovato
V
V
Redgwell
R J
RJ
D002236:Carbohydrate Conformation; D002850:Chromatography, Gel; D003069:Coffee / Q000737:chemistry*; D005685:Galactans / Q000032:analysis; D005690:Galactose / Q000031:analogs & derivatives; D008351:Mannans / Q000032:analysis; D008358:Mannose / Q000032:analysis; D009005:Monosaccharides / Q000032:analysis; D011134:Polysaccharides / Q000032:analysis* / Q000302:isolation & purification; D012995:Solubility
D003069:Coffee; D005685:Galactans; D008351:Mannans; D009005:Monosaccharides; D011134:Polysaccharides; C012990:galactomannan; D008358:Mannose; C005653:arabinogalactan; D005690:Galactose