Adipsin and the glucose transporter GLUT4 traffic to the cell surface via independent pathways in adipocytes.
Insulin increases the exocytosis of many soluble and membrane proteins in adipocytes. This may reflect a general effect of insulin on protein export from the trans Golgi network. To test this hypothesis, we have compared the trafficking of the secreted serine protease adipsin and the integral membrane proteins GLUT4 and transferrin receptors in 3T3-L1 adipocytes. We show that adipsin is secreted from the trans Golgi network to the endosomal system, as ablation of endosomes using transferrin-HRP conjugates strongly inhibited adipsin secretion. Phospholipase D has been implicated in export from the trans Golgi network, and we show that insulin stimulates phospholipase D activity in these cells. Inhibition of phospholipase D action with butan-1-ol blocked adipsin secretion and resulted in accumulation of adipsin in trans Golgi network-derived vesicles. In contrast, butan-1-ol did not affect the insulin-stimulated movement of transferrin receptors to the plasma membrane, whereas this was abrogated following endosome ablation. GLUT4 trafficking to the cell surface does not utilise this pathway, as insulin-stimulated GLUT4 translocation is still observed after endosome ablation or inhibition of phospholipase D activity. Immunolabelling revealed that adipsin and GLUT4 are predominantly localised to distinct intracellular compartments. These data suggest that insulin stimulates the activity of the constitutive secretory pathway in adipocytes possibly by increasing the budding step at the TGN by a phospholipase D-dependent mechanism. This may have relevance for the secretion of other soluble molecules from these cells. This is not the pathway employed to deliver GLUT4 to the plasma membrane, arguing that insulin stimulates multiple pathways to the cell surface in adipocytes.
Millar
C A
CA
Division of Biochemistry and Molecular Biology, Davidson Building, University of Glasgow, Glasgow G12 8QQ, UK.
Meerloo
T
T
Martin
S
S
Hickson
G R
GR
Shimwell
N J
NJ
Wakelam
M J
MJ
James
D E
DE
Gould
G W
GW
D017667:Adipocytes / Q000378:metabolism*; D000818:Animals; D000440:Butanols / Q000494:pharmacology; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D011416:Complement Factor D; D003847:Deoxyglucose / Q000493:pharmacokinetics; D011992:Endosomes / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D051272:Glucose Transporter Type 1; D051275:Glucose Transporter Type 4; D006056:Golgi Apparatus / Q000378:metabolism; D007328:Insulin / Q000378:metabolism; D051379:Mice; D018613:Microscopy, Confocal; D016253:Microscopy, Immunoelectron; D008954:Models, Biological; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D009124:Muscle Proteins; D010739:Phospholipase D / Q000037:antagonists & inhibitors / Q000378:metabolism; D021381:Protein Transport; D011990:Receptors, Transferrin / Q000378:metabolism; D012697:Serine Endopeptidases / Q000378:metabolism*; D013347:Subcellular Fractions; D013997:Time Factors; D014168:Transferrin / Q000378:metabolism; D021601:trans-Golgi Network / Q000378:metabolism
D000440:Butanols; D051272:Glucose Transporter Type 1; D051275:Glucose Transporter Type 4; D007328:Insulin; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins; D011990:Receptors, Transferrin; C495693:Slc2a4 protein, mouse; D014168:Transferrin; D003847:Deoxyglucose; D010739:Phospholipase D; D012697:Serine Endopeptidases; D011416:Complement Factor D; C493491:complement factor D, mouse