Metabolic activation of benzo[a]pyrene by a human hepatoma cell line.
The liver-derived human cell line, Hep G2, has high benzo[a]pyrene-metabolizing activity and converts benzo[a]pyrene to intermediates that are mutagenic and that bind to DNA. This cell line will be useful for studying metabolic activation of polycyclic aromatic hydrocarbons and other xenobiotics by human tissue and as an activation system in short-term screening assays for identifying compounds with carcinogenic potential for humans.
Diamond
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L
The Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104, USA.
Kruszewski
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F
Aden
D P
DP
Knowles
B B
BB
Baird
W M
WM
D000818:Animals; D001564:Benzo(a)pyrene / Q000302:isolation & purification / Q000378:metabolism / Q000493:pharmacokinetics* / Q000633:toxicity; D001711:Biotransformation; D002273:Carcinogens / Q000378:metabolism / Q000493:pharmacokinetics* / Q000633:toxicity; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D018920:Coculture Techniques; D006224:Cricetinae; D003412:Cricetulus; D018736:DNA Adducts / Q000096:biosynthesis / Q000302:isolation & purification; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism*; D008168:Lung / Q000166:cytology / Q000187:drug effects; D016296:Mutagenesis / Q000187:drug effects; D009152:Mutagenicity Tests; D009153:Mutagens / Q000378:metabolism / Q000493:pharmacokinetics / Q000633:toxicity; D014407:Tumor Cells, Cultured
D002273:Carcinogens; D018736:DNA Adducts; D009153:Mutagens; C056612:benzo(a)pyrene-DNA adduct; D001564:Benzo(a)pyrene