title: [Reverse transcriptase polymerase chain reaction and immunocytochemistry of bone marrow aspiration specimen and peripheral blood for detection of microdisseminated prostatic carcinoma. A comparative analysis].
abstract: Reverse transcriptase-polymerase chain reaction (RT-PCR) assay for prostate-specific antigen and immunocytochemistry for cytokeratin-18 (CK-18) are tests for the detection of microdisseminated carcinoma of the prostate. Bone marrow aspirates and peripheral venous blood from 50 patients with clinically organ-confined prostate cancer were examined. The rate of positive results was independent of the pT stage, serum PSA, and previous antiandrogen treatment. RT-PCR and immunocytochemistry have to be tested under standardized conditions in prospective trials, and the results have to be compared to the serum PSA follow-up.
authors.affiliation: Urologische Klinik, Universitätsklinikum, RWTH Aachen.
authors.forename: H
authors.initials: H
authors.lastname: Borchers
authors.forename: J M
authors.initials: JM
authors.lastname: Wolff
authors.forename: B
authors.initials: B
authors.lastname: Brehmer
authors.forename: A J
authors.initials: AJ
authors.lastname: Beniers
authors.forename: G
authors.initials: G
authors.lastname: Jakse
mesh_terms: D000368:Aged; D001707:Biopsy, Needle; D001853:Bone Marrow / Q000473:pathology; D019046:Bone Marrow Neoplasms / Q000473:pathology / Q000556:secondary*; D006801:Humans; D007633:Keratins / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D009360:Neoplastic Cells, Circulating; D011237:Predictive Value of Tests; D011379:Prognosis; D011471:Prostatic Neoplasms / Q000473:pathology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D007633:Keratins
vernacular_title: Reverse Transkriptase-Polymerase-Kettenreaktion und Immunozytochemie an Knochenmarkaspiraten und peripherem Blut zum Nachweis mikrodisseminierter Prostatakarzinome. Eine vergleichende Analyse.