title: Diagnostic value of intraoperative swabs of heart valves in infective endocarditis.
abstract: OBJECTIVE
  Intraoperative swabs of heart valves are obtained regularly from patients undergoing heart valve surgery for infective endocarditis (IE) in order to confirm the preoperative diagnosis and to adjust the antibiotic regimen. The study aim was to assess the diagnostic value of intraoperative swabs of heart valves in IE.
  
  
  METHODS
  A total of 83 patients was referred for surgical treatment of active IE between October 1994 and May 1999. Preoperatively, microorganisms were isolated using a minimum of two positive blood cultures; results were compared with those obtained from intraoperative heart valve swab cultures.
  
  
  RESULTS
  Preoperatively, 73 patients (88%) had a positive blood culture, and 10 (12%) had culture-negative endocarditis. The intraoperative swab confirmed the preoperative diagnosis in 31 cases (37%). Bacteria were isolated in three of the ten patients with preoperative culture-negative IE. Despite positive histopathological findings in seven patients, no microorganisms were cultured either pre- or intraoperatively. Among the remaining 42 patients (51%) with active IE, 25 valve cultures were sterile and 17 valve swabs were presumed to be contaminated.
  
  
  CONCLUSIONS
  In patients with active IE in whom the causative agent could be isolated and identified before surgery, intraoperative valve swabs did not contribute further to patient management. In isolating contaminants, the risk of inappropriate modification of the antibiotic regimen is imminent. The diagnostic validity in culture-negative IE appears negligible.
authors.affiliation: Department of Internal Medicine/Cardiology, University of Leipzig, Heart Center GmbH, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Thiele
authors.forename: R
authors.initials: R
authors.lastname: Hambrecht
authors.forename: B
authors.initials: B
authors.lastname: Lauer
authors.forename: M
authors.initials: M
authors.lastname: Weinert
authors.forename: F W
authors.initials: FW
authors.lastname: Mohr
authors.forename: G
authors.initials: G
authors.lastname: Schuler
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016470:Bacteremia / Q000382:microbiology / Q000601:surgery; D001431:Bacteriological Techniques; D004697:Endocarditis, Bacterial / Q000382:microbiology* / Q000601:surgery; D005260:Female; D006351:Heart Valves / Q000382:microbiology* / Q000601:surgery; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D011237:Predictive Value of Tests