Sensitive and rapid method for the simultaneous quantification of the HIV-protease inhibitors indinavir, nelfinavir, ritonavir, and saquinavir in human plasma by reversed-phase liquid chromatography.
A rapid, sensitive, and specific liquid chromatography method for the simultaneous determination of four protease inhibitors (indinavir, nelfinavir, ritonavir, and saquinavir) in human plasma is described. After a liquid-liquid extraction with terbutyl methyl ether and a sequential washing of the reconstituted sample with hexane, protease inhibitors are separated on a phenyl column using a simple binary mobile phase of ammonium acetate buffer:acetonitrile (48:52) (pH = 7.5) with an ultraviolet detection at 260 nm. The standard curves are linear in the range 0.025-1 microg/mL for saquinavir, 0.1-4 microg/mL for indinavir and nelfinavir, and 0.25-10 microg/mL for ritonavir, with an average recovery ranging from 79% to 99%, and with both low interday and intraday coefficients of variation (<15%). This assay is simple, rapid (15-minute interval between runs) , and useful for therapeutic monitoring of the protease inhibitors on a routine basis.
Bouley
M
M
Department of Pharmacotoxicology, Avicenne University Hospital, Bobigny, France.
Briere
C
C
Padoin
C
C
Petitjean
O
O
Tod
M
M
D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D016903:Drug Monitoring; D017320:HIV Protease Inhibitors / Q000097:blood*; D006801:Humans; D007202:Indicators and Reagents; D019469:Indinavir / Q000097:blood; D019888:Nelfinavir / Q000097:blood; D012015:Reference Standards; D019438:Ritonavir / Q000097:blood; D019258:Saquinavir / Q000097:blood; D013056:Spectrophotometry, Ultraviolet
D017320:HIV Protease Inhibitors; D007202:Indicators and Reagents; D019469:Indinavir; D019888:Nelfinavir; D019258:Saquinavir; D019438:Ritonavir