Effects of zinc ions on Ca2+ uptake during histamine-induced contraction in guinea-pig taenia coli.
The present study was undertaken to clarify the effects of zinc ions (Zn2+) on Ca2+ uptake during histamine-induced contraction in guinea-pig taenia coli. Zn2+ (0.3 mM) had smaller effect on the initial phasic response to 10(-5) M histamine accompanied with decreased Ca uptake at the high affinity sites, while it inhibited the tonic response by inhibiting Ca2+ uptake at the low affinity sites. After the first stimulation with histamine in the presence of Zn2+, the Ca2+ binding at high affinity sites was not significantly recovered in the presence of Zn2+. A second stimulation with histamine in the presence of Zn2+ did not elicit any phasic response. Furthermore, after beta-escin treatment of the fibres, which leaves receptor-coupled signal transduction including the Ca2+ storage sites intact, the contraction due to 10(-5) M histamine was not so affected by 0.3 mM Zn2+, but, after resuspension in a solution of pCa 5, 10(-5) M histamine of second application did not elicit the contraction in the presence of Zn2+. These results suggested that Zn2+ does not affect the histamine-induced Ca2+ release from the intracellular storage sites in taenia coli. However, when Zn2+ is present, Ca2+ is not supplied to the storage sites for the phasic response due to second stimulation with histamine. In addition, Zn2+ reduced the tonic response to histamine mainly by inhibiting Ca2+ influx through receptor-operated and/or voltage-dependent Ca2+ channels.
Nasu
T
T
Department of Veterinary Pharmacology, Faculty of Agriculture, Yamaguchi University, Japan. nasu@agr.yamaguchi-u.ac.jp
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002712:Chlorides / Q000494:pharmacology*; D003106:Colon / Q000187:drug effects / Q000502:physiology*; D004594:Electrophysiology; D004928:Escin / Q000494:pharmacology; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology*; D007811:Lanthanum / Q000494:pharmacology; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology*; D017967:Zinc Compounds / Q000494:pharmacology*
D002712:Chlorides; D017967:Zinc Compounds; D004928:Escin; D007811:Lanthanum; D006632:Histamine; C016837:zinc chloride; D002118:Calcium