title: Overexpression of the calcium-dependent protein kinase OsCDPK2 in transgenic rice is repressed by light in leaves and disrupts seed development.
abstract: Independent transgenic rice lines overexpressing the rice CDPK isoform OsCDPK2 were generated by particle bombardment. High levels of OsCDPK2 were detected in leaves removed from etiolated plants, as well as in stems and flowers. However, there was no overexpression in green leaves that had been exposed to light, confirming that OsCDPK2 protein stability was subject to light regulation. The morphological phenotype of transgenic plants producing high levels of recombinant OsCDPK2 was normal until the onset of seed development. Flowers developed normally, producing well-shaped ovaries and stigmas, and mature anthers filled with pollen grains. However, seed formation in these plants was strongly inhibited, with only 3-7% of the flowers producing seeds. Seed development was arrested at an early stage. We discuss these data with respect to the possible requirement for specific CDPK isoforms during rice seed development.
authors.affiliation: Department of Botany, University of Milan, Milano, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Morello
authors.forename: M
authors.initials: M
authors.lastname: Frattini
authors.forename: S
authors.initials: S
authors.lastname: Gianì
authors.forename: P
authors.initials: P
authors.lastname: Christou
authors.forename: D
authors.initials: D
authors.lastname: Breviario
mesh_terms: D002135:Calcium-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D008027:Light; D012275:Oryza / Q000201:enzymology / Q000235:genetics*; D018515:Plant Leaves / Q000201:enzymology* / Q000235:genetics; D010940:Plant Proteins; D030821:Plants, Genetically Modified; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D012639:Seeds / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*
chemical_list: D002135:Calcium-Binding Proteins; D010940:Plant Proteins; D011494:Protein Kinases