Beta-VLDL protects against A beta(1-42) and apoE toxicity in human SH-SY5Y neuroblastoma cells.
The toxic effects of beta-amyloid (A beta) (1-42), apolipoprotein E (apoE) isoforms, and apoE/A beta complexes were studied in human SH-SY5Y neuroblastoma cells and fibroblasts using MTT reduction. In SH-SY5Y cells, A beta(1-42) gave time-dependent toxicity over 2-48 h, which was reduced by co-incubation with rabbit beta-very low density lipoproteins (beta-VLDL). Human recombinant apoE3 and E4 isoforms were also toxic by themselves and also potentiated A beta effects when used alone, but not when associated with beta-VLDL. None of the treatments were toxic to human fibroblasts. These results suggest that beta-VLDL has a protective role on A beta-induced neurotoxicity and that the status of apoE or the conformation of lipoprotein containing apoE particles may be important for determining the contribution of apoE to neurodegeneration.
Cedazo-Mínguez
A
A
Karolinska Institutet, NEUROTEC, Section for Geriatric Medicine, NOVUM, Huddinge, Sweden.
Hüttinger
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M
Cowburn
R F
RF
D016229:Amyloid beta-Peptides / Q000633:toxicity*; D001057:Apolipoproteins E / Q000633:toxicity*; D004396:Coloring Agents; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D008079:Lipoproteins, VLDL / Q000494:pharmacology*; D009447:Neuroblastoma; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000494:pharmacology; D010446:Peptide Fragments / Q000633:toxicity*; D012867:Skin / Q000166:cytology; D013778:Tetrazolium Salts; D013844:Thiazoles; D014407:Tumor Cells, Cultured
D016229:Amyloid beta-Peptides; D001057:Apolipoproteins E; D004396:Coloring Agents; D008079:Lipoproteins, VLDL; D018696:Neuroprotective Agents; D010446:Peptide Fragments; D013778:Tetrazolium Salts; D013844:Thiazoles; C075222:amyloid beta-protein (1-42); C022616:thiazolyl blue