Transfer of platelet-derived growth factor-BB gene by gene gun increases contraction of collagen lattice by fibroblasts in diabetic and non-diabetic human skin.
We have used an in vitro model of wound contraction, the fibroblast-populated collagen lattice, to examine the effect of platelet-derived growth factor BB (PDGF-BB) and PDGF-BB gene transfer by gene gun on the contraction of lattices composed of either diabetic or non-diabetic human fibroblasts. The area of collagen lattice and DNA synthesis were measured in 12 specimens. There were significant increases in lattice contraction with increasing doses of PDGF-BB and fibroblasts transfected with the PDGF-BB gene compared with control (p < 0.01). DNA synthesis of the non-diabetic and diabetic fibroblast lattices showed significantly increased incorporation of tritiated thymidine with increasing doses of PDGF-BB and fibroblasts transfected with the PDGF-BB compared with controls (p < 0.05). The effect of PDGF-BB gene transfer on diabetic and non-diabetic fibroblasts was similar to that of 20 ng/ml or less of PDGF-BB.
Kuwahara
H
H
Department of Plastic and Reconstructive Surgery, Hokkaido University, Sapporo, Japan.
Mitchell
A T
AT
Macklin
M D
MD
Zhao
J
J
Listengarten
D
D
Phillips
L G
LG
D000077214:Becaplermin; D019470:Biolistics; D002478:Cells, Cultured; D003094:Collagen / Q000648:ultrastructure*; D004247:DNA / Q000096:biosynthesis; D003924:Diabetes Mellitus, Type 2 / Q000473:pathology*; D005347:Fibroblasts / Q000502:physiology*; D006801:Humans; D010982:Platelet-Derived Growth Factor / Q000235:genetics*; D020574:Proto-Oncogene Proteins c-sis; D012867:Skin / Q000166:cytology*; D014162:Transfection; D014945:Wound Healing / Q000502:physiology*
D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D003094:Collagen; D004247:DNA