title: Staphylococcal surface display and its applications.
abstract: Novel surface proteins can be introduced onto the bacterial cell surface by recombinant means. Here, we describe the development of such display systems for two food-grade bacteria, Staphylococcus carnosus and Staphylococcus xylosus, and present how such engineered bacteria can be used in different applications. A study will be described in which such staphylococci were employed as vaccine delivery vehicles to elicit protective antibody responses to respiratory syncytial virus (RSV). The use of surface-engineered staphylococci as novel microbial biocatalysts, as a new type of whole-cell diagnostic devices or for adsorption of metal ions with potential environmental or biosensor applications, will also be discussed.
authors.affiliation: Department of Biotechnology, Kungliga Tekniska Högskolan, Stockholm, Sweden. stefans@biochem.kth.se
authors.forename: S
authors.initials: S
authors.lastname: Ståhl
authors.forename: A
authors.initials: A
authors.lastname: Robert
authors.forename: E
authors.initials: E
authors.lastname: Gunneriusson
authors.forename: H
authors.initials: H
authors.lastname: Wernérus
authors.forename: F
authors.initials: F
authors.lastname: Cano
authors.forename: S
authors.initials: S
authors.lastname: Liljeqvist
authors.forename: M
authors.initials: M
authors.lastname: Hansson
authors.forename: T N
authors.initials: TN
authors.lastname: Nguyen
authors.forename: P
authors.initials: P
authors.lastname: Samuelson
mesh_terms: D000818:Animals; D004269:DNA, Bacterial; D004333:Drug Administration Routes; D004798:Enzymes / Q000235:genetics / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics; D008670:Metals / Q000378:metabolism; D009092:Mucous Membrane; D010957:Plasmids; D011995:Recombination, Genetic; D013210:Staphylococcus / Q000235:genetics*; D014614:Vaccines, Synthetic
chemical_list: D004269:DNA, Bacterial; D004798:Enzymes; D008670:Metals; D014614:Vaccines, Synthetic