title: Inhibition of the antibody responses to rat blood transfusion antigens in mice by boar seminal immunosuppressive fraction.
abstract: OBJECTIVE
  Immunosuppressive fraction of boar seminal vesicle fluid (ISF) was tested to muffle primary and secondary antibody responses to xenotranfusions. Contemporaneously, heparin non-binding fraction of seminal plasma (H- fraction), presumed to be identical to ISF, was used to support the results.
  
  
  METHODS
  To study their similarity, ISF and H- fraction were analyzed by high-performance liquid chromatography and the separated proteins by N-terminal sequencing. In sera of mice treated with ISF or H- fraction, the productions of antibodies against rat erythrocytes and blood serum were evaluated by enzyme-linked immunosorbent assay (ELISA). The productions of IgM, IgA, and IgG subclasses were followed by sandwich ELISA.
  
  
  RESULTS
  ISF and H- fraction were proved to be equal complexes of porcine seminal plasma (PSP) proteins PSP I and PSP II. Both inhibited antibody responses to rat erythrocytes and serum and the concentrations of IgM, IgG, IgG1 and IgG2 after the first transfusion with a long-lasting effect. Both suppressed the secondary antibody production if applied before the second transfusion. IgA and IgG3 stayed uninfluenced. ISF and H- fraction had an equal immunosuppressive effect.
  
  
  CONCLUSIONS
  ISF was characterized biochemically, found to be identical to H- fraction, and determined to be powerful in overcoming unwanted exaggerated antibody responses to xenotransfusion.
authors.affiliation: Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague. veselsky@img.cas.cz
authors.forename: L
authors.initials: L
authors.lastname: Veselský
authors.forename: V
authors.initials: V
authors.lastname: Jonáková
authors.forename: J
authors.initials: J
authors.lastname: Dostál
authors.forename: V
authors.initials: V
authors.lastname: Holán
authors.forename: Z
authors.initials: Z
authors.lastname: Voburka
authors.forename: J
authors.initials: J
authors.lastname: Drahorád
authors.forename: P
authors.initials: P
authors.lastname: Manásková
authors.forename: B
authors.initials: B
authors.lastname: Zelezná
mesh_terms: D000818:Animals; D000910:Antibodies, Heterophile / Q000097:blood*; D015478:Antigens, Heterophile / Q000276:immunology*; D001769:Blood / Q000276:immunology*; D001803:Blood Transfusion; D005260:Female; D007114:Immunization; D007132:Immunoglobulin Isotypes / Q000097:blood; D007166:Immunosuppressive Agents / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011506:Proteins / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012661:Semen / Q000276:immunology*; D029589:Seminal Plasma Proteins; D013552:Swine
chemical_list: D000910:Antibodies, Heterophile; D015478:Antigens, Heterophile; D007132:Immunoglobulin Isotypes; D007166:Immunosuppressive Agents; D011506:Proteins; D029589:Seminal Plasma Proteins