Staphylococcal surface display and its applications.
Novel surface proteins can be introduced onto the bacterial cell surface by recombinant means. Here, we describe the development of such display systems for two food-grade bacteria, Staphylococcus carnosus and Staphylococcus xylosus, and present how such engineered bacteria can be used in different applications. A study will be described in which such staphylococci were employed as vaccine delivery vehicles to elicit protective antibody responses to respiratory syncytial virus (RSV). The use of surface-engineered staphylococci as novel microbial biocatalysts, as a new type of whole-cell diagnostic devices or for adsorption of metal ions with potential environmental or biosensor applications, will also be discussed.
Ståhl
S
S
Department of Biotechnology, Kungliga Tekniska Högskolan, Stockholm, Sweden. stefans@biochem.kth.se
Robert
A
A
Gunneriusson
E
E
Wernérus
H
H
Cano
F
F
Liljeqvist
S
S
Hansson
M
M
Nguyen
T N
TN
Samuelson
P
P
D000818:Animals; D004269:DNA, Bacterial; D004333:Drug Administration Routes; D004798:Enzymes / Q000235:genetics / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics; D008670:Metals / Q000378:metabolism; D009092:Mucous Membrane; D010957:Plasmids; D011995:Recombination, Genetic; D013210:Staphylococcus / Q000235:genetics*; D014614:Vaccines, Synthetic
D004269:DNA, Bacterial; D004798:Enzymes; D008670:Metals; D014614:Vaccines, Synthetic