Lysis of Gymnodinium breve by cultures of the green alga Nannochloris eucaryotum.
Laboratory cultures of Florida's red tide organism, Gymnodinium breve, were killed by the green alga Nannochloris eucaryotum. Studies involved organism-organism interaction as well as organism-cell-free culture (N. eucaryotum) interaction. Both studies demonstrated that N. eucaryotum adversely affected Florida's red tide organism. The lysis has been attributed to compounds called APONINs (apparent oceanic naturally occurring cytolins). N. eucaryotum crude APONIN was extracted from cell-free cultures, partially purified and fractionated. The fractions were bioassayed against G. breve, and 'fingerprints' of the deleterious fractions were obtained.
Pérez
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Department of Chemistry, University of South Florida, Tampa 33620, USA.
Sawyers
W G
WG
Martin
D F
DF
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D000460:Chlorophyta / Q000737:chemistry* / Q000166:cytology; D018920:Coculture Techniques; D004141:Dinoflagellida / Q000166:cytology / Q000187:drug effects*; D008401:Gas Chromatography-Mass Spectrometry; D010936:Plant Extracts / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology
D010936:Plant Extracts; C014340:aponin