Spectrophotometric determination of benazepril in tablets.
A simple and sensitive spectrophotometric method has been developed for the determination of benazepril HCl in pharmaceutical formulations. The method is based on the reaction of the drug with potassium permanganate in the presence of sodium hydroxide to produce a bluish-green colored species measurable at 609.4 nm. The absorbance-concentration plot is linear over the range 1-8 microg ml(-1) with minimum detectability of 0.1 microg ml(-1) (2.17 x 10(-7) M). The molar absorptivity was 4.07 x 10(4) l mol(-1) cm(-1) with correlation coefficient (n = 6) of 0.9991. The different experimental parameters affecting the development and stability of the color were studied carefully and optimized. The proposed method was applied successfully to the determination of benazepril in its dosage forms, the percentage recoveries +/- SD (n = 9) were 99.79 +/- 1.40 and 100.50 +/- 1.48 for tablets containing 10 and 20 mg, respectively. The results obtained were in good agreement with those obtained using a reference spectrophotometric method. The proposed method could be applied to the determination of benazepril in presence of the co-formulated drug, hydrochlorothiazide. A proposal of the reaction pathway was presented.
Belal
F
F
Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia. ffbelal@ksu.edu.sa
Al-Zaagi
I A
IA
Abounassif
M A
MA
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000032:analysis*; D001552:Benzazepines / Q000032:analysis*; D002138:Calibration; D007202:Indicators and Reagents; D011196:Potassium Permanganate; D013056:Spectrophotometry, Ultraviolet; D013607:Tablets; D013696:Temperature
D000806:Angiotensin-Converting Enzyme Inhibitors; D001552:Benzazepines; D007202:Indicators and Reagents; D013607:Tablets; D011196:Potassium Permanganate; C044946:benazepril