Quantification of beta-trace protein and detection of transferrin isoforms in mixtures of cerebrospinal fluid and blood serum as models of rhinorrhea and otorrhea diagnosis.
Different mixtures from a serum pool and a cerebrospinal fluid (CSF) pool were used as models to study CSF contamination in secretions by determining two CSF specific proteins: beta-trace protein (beta-TP) and the asialo-transferrin (a-Tf) band which was detected by isoelectric focusing (IEF) with Tf specific immunofixation. Beta-TP and total Tf were measured immunonephelometrically. Secretion/serum ratios of beta-TP content > 2.0 indicated CSF contaminations with > or = 5% (v/v) CSF; this was confirmed by detecting the a-Tf band by IEF. Reliable a-Tf bands were only revealed with secretion/serum rations of Tf contents < 0.1, indicating an interference of major sialo-Tf fractions with the a-TF band detection in the sample. For CSF detection in rhinorrhea and otorrhea, complementary use of beta-TP assay and a-Tf assay is recommended. Preanalytically, dilution or concentration of the sample as well as denaturation of Tf and beta-TP should be prevented by optimizing sample collection.
Kleine
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Med. Zentrum für Nervenheilkunde, Funktionsbereich Neurochemie, Universität Marburg, Germany.
Damm
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Althaus
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D001212:Asialoglycoproteins / Q000097:blood* / Q000134:cerebrospinal fluid*; D001798:Blood Proteins / Q000032:analysis; D002490:Central Nervous System / Q000473:pathology; D002558:Cerebrospinal Fluid Otorrhea / Q000097:blood / Q000134:cerebrospinal fluid / Q000175:diagnosis*; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis; D002559:Cerebrospinal Fluid Rhinorrhea / Q000097:blood / Q000134:cerebrospinal fluid / Q000175:diagnosis*; D006259:Craniocerebral Trauma / Q000473:pathology; D006801:Humans; D019746:Intramolecular Oxidoreductases / Q000097:blood* / Q000134:cerebrospinal fluid*; D007525:Isoelectric Focusing; D054834:Lipocalins; D020033:Protein Isoforms / Q000097:blood / Q000134:cerebrospinal fluid; D013048:Specimen Handling; D014168:Transferrin / Q000031:analogs & derivatives* / Q000134:cerebrospinal fluid*
D001212:Asialoglycoproteins; D001798:Blood Proteins; D002556:Cerebrospinal Fluid Proteins; D054834:Lipocalins; D020033:Protein Isoforms; D014168:Transferrin; C017031:asialotransferrins; D019746:Intramolecular Oxidoreductases; C022466:prostaglandin R2 D-isomerase