title: Viral pathogenesis in chicken embryos and tumor induction in chickens after in ovo exposure to serotype 1 Marek's disease virus.
abstract: We examined the susceptibility of late-stage chicken embryos to infection with oncogenic serotype 1 Marek's disease virus (MDV 1). Intravenous inoculation of MDV 1 at embryonic day (ED) 16 resulted in significant replication of the virus in embryonic tissues. Within 5 days of virus exposure, pp38 viral antigen (pp38) was detected in embryonic bursae and MDV 1 was isolated by plaque assay from the spleens, thymuses, and bursae of embryos. The pathogenesis of MDV 1 after intravenous inoculation at ED 16 was similar to that in chicks exposed to MDV 1 after hatching. In contrast to the response of the embryo to intravenous inoculation, embryos exposed to MDV 1 by the amniotic route did not develop detectable pp38, nor could the virus be isolated from the embryonic tissues by plaque assay. These results show that the route of inoculation of MDV 1 in the embryos is critical for allowing the virus to come in contact with target cells.
authors.affiliation: Department of Veterinary PathoBiology, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108, USA.
authors.forename: C A
authors.initials: CA
authors.lastname: St  Hill
authors.forename: J M
authors.initials: JM
authors.lastname: Sharma
mesh_terms: D000653:Amniotic Fluid; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D017209:Apoptosis; D001835:Body Weight; D002642:Chick Embryo; D002645:Chickens; D005260:Female; D016679:Genome, Viral; D008381:Herpesvirus 2, Gallid / Q000235:genetics / Q000502:physiology*; D015994:Incidence; D009929:Organ Size; D010053:Ovary / Q000821:virology; D010750:Phosphoproteins / Q000032:analysis; D011201:Poultry Diseases / Q000196:embryology / Q000401:mortality / Q000473:pathology / Q000635:transmission / Q000821:virology*; D014412:Tumor Virus Infections / Q000196:embryology / Q000401:mortality / Q000473:pathology / Q000635:transmission / Q000662:veterinary* / Q000821:virology; D010948:Viral Plaque Assay; D014779:Virus Replication
chemical_list: D000956:Antigens, Viral; D010750:Phosphoproteins; C071698:phosphoprotein pp38, Marek's disease virus