Prevention of colon carcinogenesis and carcinoma metastasis by orally administered bovine lactoferrin in animals.
Bovine lactoferrin (bLF), a milk protein known to have bacteriostatic properties was examined for its preventive effects on colon and other organ carcinogenesis and experimental metastasis. (Experiment 1) The influence on colon carcinogenesis was investigated in male rats treated with azoxymethane (AOM), then received 2 or 0.2% bLF for 36 weeks. Significant reduction in the incidence (27% and 46% of the control, respectively) and number of adenocarcinomas of the large intestine was observed. (Experiment 2) In BALB/c mice bearing subcutaneous (s.c.) implants of colon carcinoma 26 (Co 26Lu). bLF demonstrated significant inhibition of spontaneous lung metastasis (approximately 43% of the control). Number of cytotoxic asialoGM1+ and CD8+ cells in white blood cells increased (171% and 122% of control, respectively) after treatment. Results of those experiments indicate that bLF remarkably prevents colon carcinogenesis and lung metastasis of colon carcinoma cells, possibly due to increasing cytotoxic cells in the peripheral blood.
Tsuda
H
H
Experimental Pathology and Chemotherapy Division, National Cancer Center Research Institute, Tokyo, Japan. htsuda@gan2.ncc.go.jp
Sekine
K
K
Takasuka
N
N
Toriyama-Baba
H
H
Iigo
M
M
D000818:Animals; D001397:Azoxymethane; D015496:CD4-Positive T-Lymphocytes; D018414:CD8-Positive T-Lymphocytes; D002417:Cattle; D018920:Coculture Techniques; D003110:Colonic Neoplasms / Q000209:etiology / Q000517:prevention & control*; D005677:G(M1) Ganglioside / Q000032:analysis; D007781:Lactoferrin / Q000008:administration & dosage / Q000627:therapeutic use*; D007962:Leukocytes / Q000276:immunology; D008175:Lung Neoplasms / Q000517:prevention & control / Q000556:secondary; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009362:Neoplasm Metastasis / Q000517:prevention & control*; D009368:Neoplasm Transplantation; D051381:Rats; D011916:Rats, Inbred F344; D014407:Tumor Cells, Cultured
D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside; D007781:Lactoferrin; D001397:Azoxymethane