title: Characterization of a bovine herpesvirus type 4 isolated from the spinal cord of a cow with astasia.
abstract: A bovine herpesvirus (BHV) strain designated B11-41, was recently isolated from the spinal cord of a cow with astasia. By BHV type specific polymerase chain reaction (PCR), a BHV-4 specific fragment was amplified from the DNA of strain B11-41. The nucleotide sequence of the PCR product revealed high homologies (98.4% and 99.8%) with those of two BHV-4 strains (Movar 33/63 and 86-068). The BamHI restriction endonuclease cleavage patterns of the B11-41 were more similar to those of BHV-4 than those of other types of BHV. BHV-4 is divided into two groups, European and American. The EcoRI, and BamHI, and HindIII restriction endonuclease analysis demonstrated that strain B11-41 was closely related to the American type of BHV-4. It was concluded that B11-41 was identified as a BHV-4 that belongs to the American group. Additionally, the results of this study may indicate that the nervous system is one of the sites of viral latency in natural infection.
authors.affiliation: National Institute of Animal Health, Ibaraki, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: K
authors.initials: K
authors.lastname: Murakami
authors.forename: Y
authors.initials: Y
authors.lastname: Inoshima
authors.forename: T
authors.initials: T
authors.lastname: Nakane
authors.forename: K
authors.initials: K
authors.lastname: Saika
authors.forename: H
authors.initials: H
authors.lastname: Sentsui
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D002460:Cell Line; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004279:DNA, Viral / Q000032:analysis; D018142:Gammaherpesvirinae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D006566:Herpesviridae Infections / Q000662:veterinary* / Q000821:virology; D008969:Molecular Sequence Data; D009422:Nervous System Diseases / Q000662:veterinary* / Q000821:virology; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013116:Spinal Cord / Q000821:virology*
chemical_list: D004279:DNA, Viral; D004262:DNA Restriction Enzymes