Frequent cytolytic T-cell responses to peptide MAGE-A10(254-262) in melanoma.
MAGE genes encode tumor-specific shared antigens that are among the most interesting candidates for cancer vaccines. Despite extensive studies, however, CD8+ T-cell responses to MAGE-derived epitopes have been detected only occasionally in cancer patients, even after vaccination. In contrast with these findings, we report here that HLA-A2 melanoma patients respond frequently to the recently identified peptide MAGE-A10(254-262). Indeed, as assessed by staining with fluorescent HLA-A2/peptide MAGE-A10(254-262) tetramers, CD8+ T cells directed against this peptide were readily detectable in a large proportion of HLA-A2+ melanoma patients. These results provide new insight into the immunogenicity of MAGE antigens and underline the potential usefulness of MAGE-A10 peptide-based cancer vaccines.
Valmori
D
D
Division of Clinical Onco-Immunology, Ludwig Institute for Cancer Research, University Hospital, Lausanne, Switzerland.
Dutoit
V
V
Rubio-Godoy
V
V
Chambaz
C
C
Liénard
D
D
Guillaume
P
P
Romero
P
P
Cerottini
J C
JC
Rimoldi
D
D
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000951:Antigens, Neoplasm; D019556:COS Cells; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D004274:DNA, Recombinant; D004305:Dose-Response Relationship, Drug; D015789:HLA-A2 Antigen / Q000235:genetics / Q000276:immunology; D006801:Humans; D008545:Melanoma / Q000276:immunology*; D009363:Neoplasm Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D009842:Oligopeptides / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology; D010957:Plasmids / Q000235:genetics; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D004274:DNA, Recombinant; D015789:HLA-A2 Antigen; C119317:MAGE-A10 antigen; D009363:Neoplasm Proteins; D009842:Oligopeptides; D014409:Tumor Necrosis Factor-alpha