title: Comparison between c-ELISA and CFT in detecting antibodies to Mycoplasma mycoides mycoides biotype SC in cattle affected by CBPP in Botswana.
abstract: Contagious bovine pleuropneumonia (CBPP), caused by Mycoplasma mycoides mycoides biotype small colony (SC) (MmmSC) appears to be making a serious comeback in Africa after successful control programs in many parts of the continent during the 1960s and 1970s. Botswana, a country that has been free from the disease for more than 50 years, was affected in 1995. An eradication policy was adopted by the Government of Botswana in which 320,000 cattle in the affected district of Ngamiland, Northwestern Botswana were slaughtered. This was followed by a restocking exercise in which 70,000 cattle were sent to the outbreak areas as replacement stock. It became necessary to carry out serosurveillance in order to ensure that the disease did not reenter Botswana and to ensure that the replacement stock remained free from the disease. The specificity and sensitivity of the complement fixation test (CFT) in Botswana was assessed in 82 cattle affected by the disease and held in a double fenced quarantine camp. The newly developed competitive ELISA was made available to the National Veterinary Laboratory (NVL) through the FAO/IAEA Joint Division in Vienna, Austria. Using postmortem lesions as the gold standard and a 2 x 2 contingency table, the two tests were compared in terms of their sensitivity and specificity in detecting antibodies to MmmSC. The CFT was found to be slightly more sensitive than the c-ELISA, and this could be related to the stage of the disease. A long-term study comparing the progression of the disease with the two tests is, therefore, essential.
authors.affiliation: National Veterinary Laboratory, P/Bag 0035, Gaborone, Botswana. wamanfu@gov.bw
authors.forename: W
authors.initials: W
authors.lastname: Amanfu
authors.forename: S
authors.initials: S
authors.lastname: Sediadie
authors.forename: K V
authors.initials: KV
authors.lastname: Masupu
authors.forename: M V
authors.initials: MV
authors.lastname: Raborokgwe
authors.forename: A
authors.initials: A
authors.lastname: Benkirane
authors.forename: R
authors.initials: R
authors.lastname: Geiger
authors.forename: F
authors.initials: F
authors.lastname: Thiaucourt
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D001902:Botswana; D002417:Cattle; D003168:Complement Fixation Tests / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D009176:Mycoplasma mycoides / Q000145:classification* / Q000302:isolation & purification; D011002:Pleuropneumonia, Contagious / Q000175:diagnosis* / Q000276:immunology / Q000517:prevention & control; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012703:Serotyping
chemical_list: D000907:Antibodies, Bacterial