title: Versican accumulation in human prostatic fibroblast cultures is enhanced by prostate cancer cell-derived transforming growth factor beta1.
abstract: We have previously demonstrated that peritumoral stromal matrix derived from prostate cancer patients who relapse after radical surgery contains elevated levels of versican. The purpose of this study was to determine whether prostate cancer cells control stromal cell secretion of versican. Serum-free conditioned medium from three prostate adenocarcinoma cell lines, LNCaP, PC3, and DU145, was added to cultures of fibroblasts established from prostatic tissue of patients with benign prostatic hyperplasia, and the medium was harvested at 24, 48, and 72 h. Immunoblotting with an antiversican core protein antibody revealed that prostatic fibroblast medium harvested at 72 h contained increased levels of versican after treatment with either LNCaP-, PC3- or DU145-conditioned medium (2.5-, 4.5-, and 5-fold, respectively) compared with control cultures. This increase in versican in the culture medium was not observed after coincubation with transforming growth factor beta1-neutralizing antisera. The results of this study suggest that prostate tumor cells induce host stromal cells to secrete increased versican levels via a paracrine mechanism mediated by transforming growth factor beta1.
authors.affiliation: Flinders Cancer Centre, Department of Surgery, Flinders University School of Medicine, Adelaide, South Australia, Australia.
authors.forename: A J
authors.initials: AJ
authors.lastname: Sakko
authors.forename: C
authors.initials: C
authors.lastname: Ricciardelli
authors.forename: K
authors.initials: K
authors.lastname: Mayne
authors.forename: W D
authors.initials: WD
authors.lastname: Tilley
authors.forename: R G
authors.initials: RG
authors.lastname: Lebaron
authors.forename: D J
authors.initials: DJ
authors.lastname: Horsfall
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D002450:Cell Communication / Q000502:physiology; D002455:Cell Division; D002478:Cells, Cultured; D011508:Chondroitin Sulfate Proteoglycans / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D018920:Coculture Techniques; D017077:Culture Media, Conditioned; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D037181:Lectins, C-Type; D008297:Male; D011467:Prostate / Q000166:cytology / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000096:biosynthesis / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1; D053675:Versicans
chemical_list: D011508:Chondroitin Sulfate Proteoglycans; D017077:Culture Media, Conditioned; D037181:Lectins, C-Type; D012333:RNA, Messenger; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; C508322:VCAN protein, human; D053675:Versicans