title: Antioxidant activity levels and oxidative stress as blood markers of allergic response to drugs.
abstract: Antioxidant enzymes work together in human blood cells against toxic reactive oxygen species. Although their relationship with several pathophysiologic processes has been stated, not much is known about the connection between antioxidant defence and allergy. This study was designed to determine the enzymatic activities and the oxidative indices in the blood and serum proteins in patients suffering from allergy to drugs. We hypothesize that serum and blood reactions may serve as useful clinical marker for the allergic state. We used enzymatic antioxidant activities, thiobarbituric acid reactive substances, and carbonyl contents of proteins as suitable markers. We determined superoxide dismutases, glutathione peroxidase and catalase activities in each cell type. After antihistaminics plus steroids were given as part of a protocol treatment, enzymatic antioxidant activities, thiobarbituric acid reactive substance levels, and carbonyl contents were used as recovering markers for the disease. We found a relationship between antioxidant enzymatic activities, thiobarbituric acid reactive substance levels, and carbonyl contents for allergic reactions belonging to several type I and type IV allergies, as well as cross-reactive intolerance to nonsteroidal anti-inflammatory drugs and an anaphylactoid reaction to a radiocontrast media. A similar pattern also exists for analogous allergic manifestations and disease-like status.
authors.affiliation: Department of Molecular Biology and Biochemistry, Faculty of Sciences, University of Málaga, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Pérez-Gómez
authors.forename: J M
authors.initials: JM
authors.lastname: Segura
authors.forename: M
authors.initials: M
authors.lastname: Blanca
authors.forename: M
authors.initials: M
authors.lastname: Asenjo
authors.forename: J M
authors.initials: JM
authors.lastname: Mates
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000975:Antioxidants; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000032:analysis; D002374:Catalase / Q000097:blood; D004342:Drug Hypersensitivity / Q000097:blood* / Q000188:drug therapy; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood*; D006634:Histamine H1 Antagonists / Q000627:therapeutic use; D006801:Humans; D008297:Male; D016555:Matched-Pair Analysis; D008875:Middle Aged; D018384:Oxidative Stress; D010544:Peroxidases / Q000097:blood*; D017382:Reactive Oxygen Species; D013256:Steroids / Q000627:therapeutic use; D013482:Superoxide Dismutase / Q000097:blood; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D001798:Blood Proteins; D006634:Histamine H1 Antagonists; D017382:Reactive Oxygen Species; D013256:Steroids; D017392:Thiobarbituric Acid Reactive Substances; D010544:Peroxidases; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase