title: A recombinant Salmonella typhimurium vaccine strain is taken up and survives within murine Peyer's patch dendritic cells.
abstract: The attenuated Salmonella typhimurium PhoPc strain is avirulent but immunogenic via the oral route in mice and is attenuated in survival in macrophage cell lines. In this study, the fate of PhoPc bacteria expressing green fluorescent protein was investigated in murine Peyer's patches. The survival of PhoPc was monitored after orogastric inoculation of BALB/c mice. Bacteria persisted for several weeks in the Peyer's patches and were also recovered from the mesenteric lymph nodes and spleen. Confocal microscopy analysis identified dendritic cells as the Peyer's patch cell type that internalized PhoPc expressing green fluorescent protein at early time points. In addition, live PhoPc were found in Peyer's patch dendritic cells and not in B cells 3 days after orogastric inoculation. Taken together, these results provide strong evidence that PhoPc is internalized and survives within Peyer's patch dendritic cells. As these cells are potent antigen-presenting cells, these data could explain the immunogenicity of S. typhimurium vaccine strains in vivo.
authors.affiliation: Swiss Institute for Cancer Research, Institute of Biochemistry, University of Lausanne, Epalinges, Switzerland.
authors.forename: S A
authors.initials: SA
authors.lastname: Hopkins
authors.forename: F
authors.initials: F
authors.lastname: Niedergang
authors.forename: I E
authors.initials: IE
authors.lastname: Corthesy-Theulaz
authors.forename: J P
authors.initials: JP
authors.lastname: Kraehenbuhl
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000382:microbiology; D015169:Colony Count, Microbial; D003713:Dendritic Cells / Q000382:microbiology*; D015870:Gene Expression; D049452:Green Fluorescent Proteins; D007202:Indicators and Reagents / Q000378:metabolism; D007413:Intestinal Mucosa / Q000382:microbiology; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008264:Macrophages / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D010581:Peyer's Patches / Q000382:microbiology* / Q000648:ultrastructure; D022562:Salmonella Vaccines / Q000276:immunology*; D012486:Salmonella typhimurium / Q000235:genetics / Q000254:growth & development / Q000276:immunology* / Q000648:ultrastructure; D014613:Vaccines, Attenuated / Q000276:immunology; D019444:Vaccines, DNA / Q000276:immunology
chemical_list: D007202:Indicators and Reagents; D008164:Luminescent Proteins; D022562:Salmonella Vaccines; D014613:Vaccines, Attenuated; D019444:Vaccines, DNA; D049452:Green Fluorescent Proteins