Quantification of C-polysaccharide in Streptococcus pneumoniae polysaccharides by high-performance anion-exchange chromatography with pulsed amperometric detection.
A sensitive method, using high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) has been developed for measurement of the C-polysaccharide contamination in Streptococcus pneumoniae capsular polysaccharides, which are part of the polysaccharides based vaccines for the prevention of pneumococcal infections. This method, based on the quantification by HPAEC-PAD of the ribitol released by aqueous hydrofluoric acid (HF) followed by trifluoro acid hydrolysis (TFA) of the pneumococcal polysaccharides is simple and provides both a qualitative and quantitative method for control of the polysaccharides.
Talaga
P
P
Aventis Pasteur, Marcy I'Etoile, France.
Moreau
M
M
D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D022242:Pneumococcal Vaccines / Q000032:analysis* / Q000276:immunology; D011134:Polysaccharides / Q000032:analysis* / Q000276:immunology*; D012255:Ribitol / Q000032:analysis; D013296:Streptococcus pneumoniae / Q000276:immunology*
D022242:Pneumococcal Vaccines; D011134:Polysaccharides; D012255:Ribitol