Secretion of hen egg white lysozyme from Kluyveromyces lactis.
Hen egg white (HEW) lysozyme was correctly processed and efficiently secreted from an alternative yeast, Kluyveromyces lactis. We constructed secretion vectors using PHO5, PGK, and LAC4 promoters, and found that the highest secretion was obtained under the direction of the PGK promoter in non-selective rich medium. K. lactis secreted HEW lysozyme with two-fold higher efficiency than S. cerevisiae, estimated by using a K. lactis-S. cerevisiae shuttle vector.
Tanaka
R
R
Laboratory of Applied and Molecular Microbiology, Faculty of Agriculture, Kagoshima University, Japan.
Ishibashi
M
M
Tokunaga
H
H
Tokunaga
M
M
D000818:Animals; D002645:Chickens; D004531:Eggs; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D005822:Genetic Vectors / Q000235:genetics; D007716:Kluyveromyces / Q000235:genetics* / Q000378:metabolism; D008837:Micrococcus / Q000378:metabolism; D009113:Muramidase / Q000235:genetics / Q000378:metabolism*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics
D011994:Recombinant Proteins; D009113:Muramidase