[The expression of CD15 mRNA CD44v6 mRNA and nm23H1 mRNA in breast cancer and their clinical significance].
OBJECTIVE
To study the relationship of the expression of CD15 mRNA and its protein, CD44v6 mRNA and nm23H1 mRNA with the clinical pathology parameter and prognosis of breast cancer, and to investigate the correlation of the expression of CD15 mRNA with CD44v6 mRNA and nm23H1 mRNA.


METHODS
Catalyzed signal amplification (CSA) method of in situ hybridization and immunohistochemistry were used to detect the expression of CD15 mRNA and its protein, CD44v6 mRNA and nm23H1 mRNA in 94 cases of breast cancer.


RESULTS
The overexpression of CD15 mRNA and its protein and CD44v6 mRNA and the low expression nm23H1 mRNA were correlated with the grading, clinical stage, lymph node metastasis, recurrence and prognosis of breast cancer. Patients who had overexpression of CD15 mRNA and CD44v6 mRNA and low expression of nm23H1 mRNA had a higher lymph node metastatic rate and a lower survival rate.


CONCLUSIONS
The expression of CD15 mRNA has a synergistic action in positive and negative regulation with that of CD44v6 mRNA and nm23H1 mRNA. Combining detection of the expression of these three mRNA is a reliable index to evaluate the metastasis, recurrence and prognosis of breast cancer.
Gu
H
H
Department of Pathology, Chinese PLA 251 Hospital, Zhangjiakou 075000, China.
Ni
C
C
Zhan
R
R
D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D018960:Hyaluronan Receptors / Q000096:biosynthesis* / Q000235:genetics; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D016256:Lewis X Antigen / Q000096:biosynthesis* / Q000235:genetics; D020559:Monomeric GTP-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D054778:NM23 Nucleoside Diphosphate Kinases; D009701:Nucleoside-Diphosphate Kinase; D012333:RNA, Messenger / Q000096:biosynthesis; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics
D018960:Hyaluronan Receptors; D016256:Lewis X Antigen; D054778:NM23 Nucleoside Diphosphate Kinases; D012333:RNA, Messenger; D014157:Transcription Factors; D009701:Nucleoside-Diphosphate Kinase; D020559:Monomeric GTP-Binding Proteins