title: Expression of intercellular adhesion molecule-1 induced by high glucose concentrations in human aortic endothelial cells.
abstract: We examined the effects of high glucose concentrations on the expression of adhesion molecules in human aortic endothelial cells. Expression levels of both mRNA and protein of intercellular adhesion molecule-1 (ICAM-1) were increased after incubation of endothelial cells with 30 mM glucose for 24 h. The effect of glucose on ICAM-1 was concentration dependent, partially attributable to osmolarity, and enhanced by glycated-collagen. Staurosporine (10 nM), epalrestat (10 microM) suppressed the expression of ICAM-1 mRNA and protein induced by high glucose to variable extents. Aminoguanidine (50 mM) suppressed the expression of ICAM-1 protein. It is thought that soluble ICAM-1 protein is produced by shedding in human aortic endothelial cells because RNA for the soluble form of ICAM-1 formed by variant splicing has not been detected. These results show that glucose is an important determinant of ICAM-1 expression in endothelial cells, and suggest that ICAM-1 molecules induced by hyperglycemia may contribute to the development of atherosclerosis in diabetes mellitus.
authors.affiliation: Department of Internal Medicine 5, Tokyo Medical University, Inashiki, Ibaraki, Japan. s-kado@tokyo-med.ac.jp
authors.forename: S
authors.initials: S
authors.lastname: Kado
authors.forename: T
authors.initials: T
authors.lastname: Wakatsuki
authors.forename: M
authors.initials: M
authors.lastname: Yamamoto
authors.forename: N
authors.initials: N
authors.lastname: Nagata
mesh_terms: D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors; D001011:Aorta / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D005947:Glucose / Q000494:pharmacology*; D006146:Guanidines / Q000494:pharmacology; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D052250:Nitric Oxide Synthase Type III; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012236:Rhodanine / Q000031:analogs & derivatives* / Q000494:pharmacology; D019311:Staurosporine / Q000494:pharmacology; D053778:Thiazolidines
chemical_list: D004791:Enzyme Inhibitors; D006146:Guanidines; D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; D053778:Thiazolidines; D018799:Intercellular Adhesion Molecule-1; C038131:epalrestat; D012236:Rhodanine; D003094:Collagen; D000449:Aldehyde Reductase; C496314:NOS3 protein, human; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III; D019311:Staurosporine; D005947:Glucose; C004479:pimagedine