[Both alpha1- and beta-adrenergic receptors participate in generation of a Ca2+-response to noradrenaline in murine brown preadipocytes].
It has been shown that in Ca(2+)-signal generation, at the addition of norepinephrine (NE) to a suspension of freshly isolated brown preadipocytes, beta-receptors participate alongside with alpha 1-adrenoreceptors. The amplitude of cell response to 10(-5)-10(-8) M NE is approximately equal to the sum of effects of alpha 1-specific agonists (oxymetazoline or cirazoline) and beta-selective agonist isoproterenol. beta-Selective antagonist nadolol practically completely prevented the effect of NE on the intracellular Ca2+ level, while phentolamine, an antagonist of alpha-receptors, caused only a approximately 25% inhibition of the cellular response.
Dolgacheva
L P
LP
Institute of Cell Biophysics RAS, Pushchino, Moscow region.
Galitovskaia
E N
EN
Baumuratov
A S
AS
Zinchenko
V P
VP
Bronnikov
G E
GE
D017667:Adipocytes / Q000187:drug effects / Q000502:physiology*; D002001:Adipose Tissue, Brown / Q000187:drug effects / Q000502:physiology*; D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000502:physiology*; D008297:Male; D051379:Mice; D009638:Norepinephrine / Q000502:physiology*; D018340:Receptors, Adrenergic, alpha-1 / Q000502:physiology*; D011943:Receptors, Adrenergic, beta / Q000502:physiology*
D000317:Adrenergic alpha-Antagonists; D000319:Adrenergic beta-Antagonists; D018340:Receptors, Adrenergic, alpha-1; D011943:Receptors, Adrenergic, beta; D002118:Calcium; D009638:Norepinephrine
V generatsii Ca2+-otveta na noradrenalin v burykh preadipotsitakh myshi uchastvuiut kak alpha1-, tak i beta-adrenoretseptory.