title: [Detection of impurities in DNA samples by using the convolution spectrum method].
abstract: OBJECTIVE
  To detect the presence of phenol, amino acids and endotoxin in DNA preparations.
  
  
  METHODS
  By using the convolution curve transformation technology, correlation coefficient discriminant which was put forward by spectrum space theory and computer information process technology, impurities in DNA samples were detected.
  
  
  RESULTS
  The convolution spectrum method compared with A260/A280 ratio method and second-derivative absorption spectrum method could be used for the semiquantitative determinations of impurities in DNA samples and the results were satisfactory.
  
  
  CONCLUSIONS
  The method was simple, convenient, fast and valuable to the biochemical analysis.
authors.affiliation: Department of Pharmaceutical Analysis, College of Pharmacy, Second Military Medical University, Shanghai 200433, China.
authors.forename: H
authors.initials: H
authors.lastname: Zheng
authors.forename: Y T
authors.initials: YT
authors.lastname: Wu
authors.forename: H S
authors.initials: HS
authors.lastname: Fang
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D004247:DNA / Q000032:analysis*; D004731:Endotoxins / Q000032:analysis*; D019800:Phenol / Q000032:analysis*; D013057:Spectrum Analysis / Q000379:methods
chemical_list: D000596:Amino Acids; D004731:Endotoxins; D019800:Phenol; D004247:DNA