Synthesis and P2Y receptor activity of a series of uridine dinucleoside 5'-polyphosphates.
A series of dinucleoside 5-polyphosphates UpnU (n = 2-7) was synthesized. Their relative potencies as agonists at the G-protein-coupled receptors P2Y1, P2Y2, P2Y4, and P2Y6 were determined by intracellular calcium measurements using fluorescent imaging techniques. The correlation of phosphate chain length to activities at these receptors is discussed.
Pendergast
W
W
Inspire Pharmaceuticals, Inc., Durham, NC 27703, USA.
Yerxa
B R
BR
Douglass
J G
JG
Shaver
S R
SR
Dougherty
R W
RW
Redick
C C
CC
Sims
I F
IF
Rideout
J L
JL
D002118:Calcium / Q000378:metabolism; D015226:Dinucleoside Phosphates / Q000138:chemical synthesis / Q000378:metabolism*; D006801:Humans; D011485:Protein Binding; D058910:Purinergic P2 Receptor Agonists; D018048:Receptors, Purinergic P2 / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship; D014161:Transduction, Genetic; D014407:Tumor Cells, Cultured; D014500:Uracil Nucleotides / Q000138:chemical synthesis / Q000378:metabolism*
D015226:Dinucleoside Phosphates; D058910:Purinergic P2 Receptor Agonists; D018048:Receptors, Purinergic P2; D014500:Uracil Nucleotides; D002118:Calcium