A small-conductance Ca(2+)-activated K+ current and Cl- current in rat dental pulp cells.
We characterized a voltage-dependent ionic current in dental pulp cells on dental pulp slices using a nystatin perforated-patch recording configuration. The outward currents in dental pulp cells were inhibited by the following channel blockers: 1) Ca(2+)-free extracellular solution containing 10 mM Ba2+, 2) extracellular 400 nM apamin and 3) extracellular 300 nM 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS). On the other hand, 15 mM tetraethylammonium (TEA) did not inhibit the outward currents. The inhibitory effects of Ca(2+)-free extracellular solution, apamin and DIDS had voltage-dependency. These results indicated that dental pulp cells expressed a small-conductance Ca(2+)-activated K+ current (SK current) and a DIDS-sensitive Cl- current. The functional significance of these channels is discussed.
Shibukawa
Y
Y
Department of Physiology, Tokyo Dental College, 1-2-2, Masago, Mihama-ku, Chiba 261-8502, Japan.
Suzuki
T
T
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000494:pharmacology; D000703:Analog-Digital Conversion; D000818:Animals; D001030:Apamin / Q000494:pharmacology; D001464:Barium / Q000494:pharmacology; D002121:Calcium Channel Blockers / Q000494:pharmacology; D015220:Calcium Channels / Q000187:drug effects / Q000502:physiology*; D018118:Chloride Channels / Q000037:antagonists & inhibitors / Q000502:physiology*; D003782:Dental Pulp / Q000166:cytology* / Q000187:drug effects; D004553:Electric Conductivity; D005260:Female; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology*; D009761:Nystatin; D009804:Odontoblasts / Q000187:drug effects; D018408:Patch-Clamp Techniques; D026902:Potassium Channel Blockers; D015221:Potassium Channels / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012815:Signal Processing, Computer-Assisted; D013223:Statistics as Topic; D019789:Tetraethylammonium / Q000494:pharmacology
D002121:Calcium Channel Blockers; D015220:Calcium Channels; D018118:Chloride Channels; D026902:Potassium Channel Blockers; D015221:Potassium Channels; D009761:Nystatin; D001030:Apamin; D001464:Barium; D019789:Tetraethylammonium; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid