Probing the MVAI methyltransferase region that interacts with DNA: affinity labeling with the dialdehyde-containing DNA duplexes.
Affinity labeling of methyltransferase MvaI by DNA duplexes containing oxidized 2'-O-beta-D-ribofuranosylcytidine or 1-beta-D-galactopyranosyl)thymine residues was performed. Partial chemical hydrolysis of the covalently bound methylase in the conjugates with the dialdehyde-containing DNA allowed us to determine the amino acid region in the C terminus of methylase MvaI that interacts with DNA.
Gritsenko
O M
OM
Department of Chemistry, Moscow State University, Russia.
Mikhailov
S N
SN
Efimtseva
E V
EV
Van Aerschot
A
A
Herdewijn
P
P
Gromova
E S
ES
D000345:Affinity Labels; D000447:Aldehydes / Q000737:chemistry*; D004247:DNA / Q000378:metabolism*; D019175:DNA Methylation; D015257:DNA-Cytosine Methylases / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D015335:Molecular Probes; D013379:Substrate Specificity
D000345:Affinity Labels; D000447:Aldehydes; D015335:Molecular Probes; D004247:DNA; C029528:DNA modification methylase EcoRII; D015257:DNA-Cytosine Methylases