Needle-type glucose microbiosensor based on glucose oxidase immobilised in an overoxidised polypyrrole film (an in-vitro study).
A fast response, needle-type glucose microbiosensor has been fabricated by a one-step electrochemical immobilisation of glucose oxidase in a polypyrrole film. The sensor shows a remarkable rejection of electroactive interferences, especially paracetamol. The maximum bias observed in the worst situation never exceeded the value of 6%. The fabrication procedure delivered very reproducible devices and the sensitivity of a newly prepared biosensor was typically 650 nA mM(-1) cm(-2). The kinetic parameters, obtained from an existing model, permitted to understand the sensor behaviour.
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Dipartimento di Chimica, Università degli Studi di Bari, Italy.
Losito
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Palmisano
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Zambonin
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CG
D001234:Aspergillus niger / Q000201:enzymology; D015374:Biosensing Techniques; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000378:metabolism*; D010084:Oxidation-Reduction; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*
D004800:Enzymes, Immobilized; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D005949:Glucose Oxidase; D005947:Glucose