Binding profile of spiramycin to oviducal proteins of laying hens.
In vitro protein binding of spiramycin (SP) in the plasma and oviducts of laying hens was studied. The data for SP were compared with those for oxytetracycline (OTC), sulphadimidine (SDD), sulphamonomethoxine (SMM) and sulphaquinoxaline (SQ). The two oviduct segments, magnum (M) and isthmus plus shell gland (IS), were collected. The soluble (cell sap) fractions from the magnum (M-S9) and the isthmus plus shell gland (IS-S9) were used as samples. Plasma protein binding was highest for SQ (81.4%) (P < 0.01), and lowest for SDD (30.9%) (P < 0.01). No M-S9 protein binding of OTC was found. The IS-S9 protein binding of SP (60.4%) was very much higher than those of OTC (0.8%), SDD (4.1%), SMM (4.0%) and SQ (12.3%) (P < 0.01). Biological half-lives of these drugs in egg albumen were directly correlated to the extent of their binding to IS proteins. Of plasma, M-S9 and IS-S9, variation in SP concentration in the ranges from 1 to 20 micrograms/ml did not alter the binding properties of the drug.
Furusawa
N
N
Faculty of Human Life Science, Osaka City University, Osaka, 558-8585, Japan.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000493:pharmacokinetics*; D000890:Anti-Infective Agents / Q000493:pharmacokinetics; D002645:Chickens / Q000378:metabolism*; D005260:Female; D009961:Orosomucoid / Q000378:metabolism; D010047:Ovalbumin / Q000378:metabolism; D010057:Oviducts / Q000378:metabolism*; D010118:Oxytetracycline / Q000493:pharmacokinetics; D011485:Protein Binding; D015572:Spiramycin / Q000493:pharmacokinetics*; D013418:Sulfamethazine / Q000493:pharmacokinetics; D013422:Sulfamonomethoxine / Q000493:pharmacokinetics; D013428:Sulfaquinoxaline / Q000493:pharmacokinetics
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D009961:Orosomucoid; D013418:Sulfamethazine; D015572:Spiramycin; D010047:Ovalbumin; D013422:Sulfamonomethoxine; D013428:Sulfaquinoxaline; D010118:Oxytetracycline