Subtyping of cytomegalovirus strains obtained from immunocompetent children.
We analyzed 33 clinical isolates of cytomegalovirus (CMV) obtained from immunocompetent Japanese children. DNA was extracted from infected MRC-5 cells and we amplified CMV glycoprotein-B (gB), major IE (MIE), DNA polymerase and glycoprotein-H (gH) genes using PCR. Among the 33 clinical isolates, 24 were identified as gB group 1, one as group 2, 6 as group 3, and 2 as simultaneous infections with group 1 and group 3 strains. Clinical isolates of CMV have also been classified into 18 MIE, 8 DNA polymerase and 15 gH genotype patterns by restriction fragment length polymorphism (RFLP). RFLP analysis is useful in molecular epidemiological studies of CMV infection in immunocompetent individuals.
Numazaki
K
K
Department of Pediatrics, Sapporo Medical University School of Medicine, Sapporo, 060-8543, Japan. numazaki@sapmed.ac.jp
Ikehata
M
M
Chiba
S
S
D003587:Cytomegalovirus / Q000145:classification* / Q000235:genetics*; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000276:immunology; D004279:DNA, Viral / Q000032:analysis; D004259:DNA-Directed DNA Polymerase / Q000235:genetics; D017781:Genes, Immediate-Early; D005838:Genotype; D006801:Humans; D007121:Immunocompetence; D007223:Infant; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D014759:Viral Envelope Proteins / Q000235:genetics
D004279:DNA, Viral; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus; C071440:glycoprotein H, Cytomegalovirus; C499358:glycoprotein H, Human cytomegalovirus; D004259:DNA-Directed DNA Polymerase