Nonenzymatic template-directed condensation of short-chained oligouridylates on a poly(A) template.
An oligouridylate with chain-length of more than three was condensed on a polyadenylate [poly(A)] template with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDAC) in imidazole buffer. The condensation reaction proceeds via the phosphorimidazolide of the oligouridylate as an intermediate. Pentauridylate [(pU)5] was converted to decauridylate [(pU)10] in 10% yield at 0 degree C for 7 days in the presence of the poly(A) template, while no coupling product was obtained in the absence of the poly(A) template. The resulting linkage of the (pU)10 was mainly 2'-5' linkage.
Sawai
H
H
Department of Chemistry, Faculty of Engineering, Gunma University, Kiryu, Gunma, 376-8515, Japan.
Wada
M
M
D001483:Base Sequence; D002234:Carbodiimides; D019418:Evolution, Chemical; D007202:Indicators and Reagents; D008956:Models, Chemical; D009843:Oligoribonucleotides / Q000737:chemistry*; D000069497:Origin of Life; D013698:Templates, Genetic; D014500:Uracil Nucleotides / Q000737:chemistry*
D002234:Carbodiimides; D007202:Indicators and Reagents; D009843:Oligoribonucleotides; D014500:Uracil Nucleotides; C027916:oligo(U); C074139:1-ethyl-3-(3-dimethylaminoethyl)carbodiimide