Identification of bovine-specific DNA in feedstuffs.
Considering the menace of transmission of bovine spongiform encephalopathy, feed components intended for cattle nutrition must be checked for the presence of bovine-derived materials. We have been using a method based on polymerase chain reaction for the identification of bovine-specific mitochondrial DNA sequences for this purpose. The specificity of the primers for polymerase chain reaction has been tested using samples of DNA of other vertebrate species, which may also be present in rendering plant products. The method allows the detection in concentrate mixtures of 0.125% of bovine-derived material. Bovine DNA at concentrations corresponding to less than 0.5% of bovine-derived material was detected in 3 of the 30 samples of concentrate mixtures collected from distributors' stores all over the Czech Republic. All 44 samples of fish meal collected from the same sources were free of bovine-derived material.
Krcmár
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P
Veterinary Research Institute, Brno, Czech Republic. krcmar@vri.cz
Rencová
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E
D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D001688:Biological Products; D002417:Cattle; D002645:Chickens; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000032:analysis*; D016643:Encephalopathy, Bovine Spongiform / Q000517:prevention & control* / Q000635:transmission; D005396:Fish Products / Q000032:analysis; D005506:Food Contamination / Q000032:analysis*; D006736:Horses; D008460:Meat; D008903:Minerals / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods; D011817:Rabbits; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D012756:Sheep; D013552:Swine
D001688:Biological Products; D017931:DNA Primers; D004272:DNA, Mitochondrial; D008903:Minerals; C027762:bone meal