Involvement of staphylococcal protein A and cytoskeletal actin in Staphylococcus aureus invasion of cultured human oral epithelial cells.
Following the coincidental discovery that beta-actin isolated from renal epithelial cells was precipitated by staphylococcal protein A (SPA), the possibility that SPA and cytoskeletal actin filaments may be involved in Staphylococcus aureus infection of epithelial cells was considered. Therefore, to clarify the potential role of SPA and actin filaments in S. aureus infection, the invasion efficiency of S. aureus was determined quantitatively by measuring the number of cfu of viable organisms recovered from cultured KB cells. S. aureus invasion was found to be time dependent (0-60 min) and increased linearly when increasing numbers of bacteria were added (10(4)-10(6) cfu/ml). However, significant variation in the level of invasion was noted in protein A-deficient S. aureus Wood 46. Cytochalasin B inhibited the invasion efficiency of S. aureus in a dose-dependent manner. The present study suggests that interaction of staphylococcal protein A and cytoskeletal actin filaments is involved in the S. aureus invasion of cultured KB cells, and this process may contribute, in part, to the intracellular movement, cell-to-cell spread and dissemination of S. aureus within human oral epithelial cells in vivo.
Jung
Kyu Yong
KY
*Department of Pharmacology, School of Medicine, †Department of Oral Microbiology, College of Dentistry, ‡Department of Histology, College of Oriental Medicine and §Center of Oriental Medicinal Science, Wonkwang University, Chonbuk 570-749 and ||Department of Dental Hygiene, Seoul Health College, Sungnam, Kyunggi 461-250, Korea.
Cha
Jeong Dan
JD
*Department of Pharmacology, School of Medicine, †Department of Oral Microbiology, College of Dentistry, ‡Department of Histology, College of Oriental Medicine and §Center of Oriental Medicinal Science, Wonkwang University, Chonbuk 570-749 and ||Department of Dental Hygiene, Seoul Health College, Sungnam, Kyunggi 461-250, Korea.
Lee
Seung Hyun
SH
*Department of Pharmacology, School of Medicine, †Department of Oral Microbiology, College of Dentistry, ‡Department of Histology, College of Oriental Medicine and §Center of Oriental Medicinal Science, Wonkwang University, Chonbuk 570-749 and ||Department of Dental Hygiene, Seoul Health College, Sungnam, Kyunggi 461-250, Korea.
Woo
Won Hong
WH
*Department of Pharmacology, School of Medicine, †Department of Oral Microbiology, College of Dentistry, ‡Department of Histology, College of Oriental Medicine and §Center of Oriental Medicinal Science, Wonkwang University, Chonbuk 570-749 and ||Department of Dental Hygiene, Seoul Health College, Sungnam, Kyunggi 461-250, Korea.
Lim
DO Seon
DS
Choi
Bong Kyu
BK
*Department of Pharmacology, School of Medicine, †Department of Oral Microbiology, College of Dentistry, ‡Department of Histology, College of Oriental Medicine and §Center of Oriental Medicinal Science, Wonkwang University, Chonbuk 570-749 and ||Department of Dental Hygiene, Seoul Health College, Sungnam, Kyunggi 461-250, Korea.
Kim
Kang Ju
KJ
*Department of Pharmacology, School of Medicine, †Department of Oral Microbiology, College of Dentistry, ‡Department of Histology, College of Oriental Medicine and §Center of Oriental Medicinal Science, Wonkwang University, Chonbuk 570-749 and ||Department of Dental Hygiene, Seoul Health College, Sungnam, Kyunggi 461-250, Korea.
D000199:Actins / Q000737:chemistry / Q000378:metabolism*; D000595:Amino Acid Sequence; D001422:Bacterial Adhesion; D015169:Colony Count, Microbial; D003571:Cytochalasin B / Q000494:pharmacology; D003599:Cytoskeleton / Q000378:metabolism; D004847:Epithelial Cells / Q000382:microbiology*; D006801:Humans; D007624:KB Cells; D008969:Molecular Sequence Data; D009061:Mouth Mucosa / Q000166:cytology / Q000382:microbiology*; D013205:Staphylococcal Protein A / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000472:pathogenicity* / Q000502:physiology
D000199:Actins; D013205:Staphylococcal Protein A; D003571:Cytochalasin B