[Inhibition of human glioma growth in nude mice by local secretion of angiostatin K(1-3)].
OBJECTIVE
To discuss the feasibility of human glioma therapy by the local secretion of angiostatin K(1-3)[AK(1-3)].


METHODS
AK(1-3) cDNA with secretive signal was inserted into polylinker sites of eukaryotic expression vector pcDNA3 to construct pcDNA-SAK(1-3); The vector was transfected into human glioma SHG44 cells by lipofectamine and the positive clone was screened by G418. The biological characters of glioma cells were examined with electron microscopy and FCM. The activity of AK(1-3) protein expressed by the SHG44 cells was examined by the endotheliocyte inhibition assay and immunofluorescence assay. When the tumor cells were implanted into nude mice, the tumor necrosis and micrangium was calculated by immunohistochemistry and electron microscopy in order to determine the influence of AK(1-3) protein to the human glioma growth.


RESULTS
The pcDNA-SAK(1-3) vector was successfully constructed and transfected into glioma cells that could express AK(1-3) protein. The tumorigenesis and angiogenesis of glioma cells in nude mice were greatly reduced (tumour end volume, experiment group 170 mm3, control group 8 120 mm3, P < 0.01; Blood vessel counting, experimental group 5.4, control group 12.2, P < 0.01).


CONCLUSIONS
The human glioma angiogenesis and growth were inhibited by the local secretion of AK(1-3). It can be further used in the treatment of other solid tumors.
Wu
J
J
Neurosurgical Institute of PLA, Xijing Hospital, Fourth Military Medical University, Xi' an 710032, China.
Zhang
X
X
Gao
D
D
D043168:Angiostatins; D000818:Animals; D001932:Brain Neoplasms / Q000473:pathology / Q000628:therapy*; D005240:Feasibility Studies; D005910:Glioma / Q000473:pathology / Q000628:therapy*; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D010446:Peptide Fragments / Q000235:genetics / Q000502:physiology*; D010958:Plasminogen / Q000235:genetics / Q000502:physiology*
D010446:Peptide Fragments; D043168:Angiostatins; D010958:Plasminogen