The tumour promoter TPA does not affect mutation to ouabain resistance in L5178Y mouse lymphoma cells.
Following treatment with ultraviolet light or ethyl methane-sulphonate mouse lymphoma L5178Y cells were cultured for different periods in the presence of non-toxic levels of the tumour promoter 12-O-tetra-decanoylphorbol-13-acetate (TPA). There were no significant changes in either the spontaneous or induced frequencies of ouabain-resistant variants recovered after TPA treatment. These results confirm that any effect of TPA on mutagenesis may be by modifying metabolic cooperation and the recovery of resistant cells.
Lasne
C
C
MRC Cell Mutation Unit and Centre for Medical Research, University of Sussex, Brighton, UK.
Cole
J
J
Arlett
C F
CF
D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D004351:Drug Resistance / Q000235:genetics; D005020:Ethyl Methanesulfonate / Q000633:toxicity; D007940:Leukemia L5178 / Q000235:genetics*; D051379:Mice; D016296:Mutagenesis / Q000187:drug effects*; D009153:Mutagens / Q000633:toxicity; D009154:Mutation; D010042:Ouabain / Q000633:toxicity*; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000528:radiation effects; D014466:Ultraviolet Rays
D002273:Carcinogens; D009153:Mutagens; D010042:Ouabain; D005020:Ethyl Methanesulfonate; D013755:Tetradecanoylphorbol Acetate