title: [Diagnostic possibilities of an immunoenzyme test-system for determining the spectrum of antibodies to structural and nonstructural hepatitis C virus antigens].
abstract: A new enzyme immunoassay EIA-HCV-Spectr test system constructed on the base of recombinant proteins and synthetic peptides allows separate detection of antibodies to E1/E2, core, HS3, NS4, and NS5 antigens of hepatitis C virus (HCV). The system is highly specific and more sensitive than the test systems used in screening studies, which allows its use as a final test for antiHCV antibodies. Antibodies to various HCV antigens were analyzed using this test system in patients with acute and chronic hepatitis C and asymptomatic donors with antiHCV. In acute hepatitis C during the first-second week after clinical manifestation, antibodies to nonstructural virus proteins are detected 3-4 times less often than in chronic hepatitis C. Acute hepatitis C is characterized by the presence of antibodies only to core antigen (66%). In chronic condition combinations of antibodies to structural and nonstructural HCV antigens predominate: core + NS4, core + NS3 + NS4, core + NS3 + NS5, core + NS4 + NS5, and core + NS3 + NS4 + NS5. In asymptomatic donors with antiHCV and in patients with chronic hepatitis C the spectra of antibodies were similar in 45.7% cases.
authors.forename: V K
authors.initials: VK
authors.lastname: Pimenov
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Afanas'ev
authors.forename: A A
authors.initials: AA
authors.lastname: Kolobov
authors.forename: S V
authors.initials: SV
authors.lastname: Zubov
authors.forename: N A
authors.initials: NA
authors.lastname: Dobrotina
authors.forename: V V
authors.initials: VV
authors.lastname: Novikov
mesh_terms: D006508:Hepatitis Antibodies / Q000032:analysis* / Q000276:immunology; D006526:Hepatitis C / Q000175:diagnosis*; D006801:Humans; D007124:Immunoenzyme Techniques; D011994:Recombinant Proteins / Q000276:immunology; D017361:Viral Nonstructural Proteins / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology*
chemical_list: D006508:Hepatitis Antibodies; D011994:Recombinant Proteins; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins
vernacular_title: Diagnosticheskie vozmozhnosti immunofermentnoĭ test-sistemy dilia opredeleniia spektra antitela k strukturnym i nestrukturnym antigenam virusa gepatita C.