title: [Conformational changes in Lassa viral proteins when exposed to antibodies and complement].
abstract: Addition of the complement to the antigen in Lassa virus ELISA stimulated the sensitivity and specificity of the assay. The effect depended on the mouse Ig subclasses. ELISA plate sensitization with IgG2a and IgG2b increased the sensitivity of ELISA 8-16 times. Protein footprinting data suggest that the complement C1q component stimulates antibody-induced conformation changes of the antigen.
authors.forename: A S
authors.initials: AS
authors.lastname: Vladyko
authors.forename: T V
authors.initials: TV
authors.lastname: Shkolina
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D003165:Complement System Proteins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D007836:Lassa virus / Q000737:chemistry* / Q000276:immunology; D051379:Mice; D011487:Protein Conformation; D023302:Protein Footprinting; D014764:Viral Proteins / Q000737:chemistry* / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D014764:Viral Proteins; D003165:Complement System Proteins
vernacular_title: Konformatsionnye izmeneniia v belkakh virusa Lassa pod deĭstviem antitel i komplementa.