title: The secreted effector protein of Salmonella dublin, SopA, is translocated into eukaryotic cells and influences the induction of enteritis.
abstract: Salmonella-induced enteritis is associated with the induction of an acute intestinal inflammatory response and net fluid secretion into the lumen of infected mucosa. Proteins secreted by the Inv/Spa type III secretion system of Salmonella play a key role in the induction of these responses. We have demonstrated recently that the Inv/Spa-secreted SopB and SopD effector proteins are translocated into eukaryotic cells via a Sip-dependent pathway and act in concert to mediate inflammation and fluid secretion in infected ileal mucosa. Mutations of both sopB and sopD significantly reduced, but did not abrogate, the enteropathogenic phenotype. This indicated that other virulence factors are involved in the induction of enteritis. In this work, we characterize SopA, a secreted protein belonging to the family of Sop effectors of Salmonella dublin. We demonstrate that SopA is translocated into eukaryotic cells and provide evidence suggesting that SopA has a role in the induction of enteritis.
authors.affiliation: Institute for Animal Health, Compton, Berkshire, UK.
authors.forename: M W
authors.initials: MW
authors.lastname: Wood
authors.forename: M A
authors.initials: MA
authors.lastname: Jones
authors.forename: P R
authors.initials: PR
authors.lastname: Watson
authors.forename: A M
authors.initials: AM
authors.lastname: Siber
authors.forename: B A
authors.initials: BA
authors.lastname: McCormick
authors.forename: S
authors.initials: S
authors.lastname: Hedges
authors.forename: R
authors.initials: R
authors.lastname: Rosqvist
authors.forename: T S
authors.initials: TS
authors.lastname: Wallis
authors.forename: E E
authors.initials: EE
authors.lastname: Galyov
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D002417:Cattle; D002460:Cell Line; D004751:Enteritis / Q000382:microbiology*; D005057:Eukaryotic Cells / Q000382:microbiology*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D012475:Salmonella / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D016415:Sequence Alignment; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; C060971:SopA protein, Bacteria