title: Synthesis and incorporation of pyrrole carboxamide nucleoside triphosphates by DNA polymerases.
abstract: We have synthesised and examined the enzymatic incorporation properties of the 5'-triphosphates of 2'-deoxyribosyl pyrrole 3-monocarboxamide (dMTP) and 2'-deoxyribosyl pyrrole 3,4-dicarboxamide (dDTP). These analogues we had hoped would behave as ambivalent base analogues in that they can present two alternative hydrogen-bonding faces either by rotation about the carboxamide group or about the glycosidic bond. The two pyrrole derivatives, dMTP and dDTP, exhibit a preference for incorporation with Klenow polymerase. They are preferentially incorporated as either A or C.
authors.affiliation: Medical Research Council, Laboratory of Molecular Biology, Cambridge, UK.
authors.forename: D
authors.initials: D
authors.lastname: Loakes
authors.forename: M J
authors.initials: MJ
authors.lastname: Guo
authors.forename: D M
authors.initials: DM
authors.lastname: Brown
authors.forename: S A
authors.initials: SA
authors.lastname: Salisbury
authors.forename: C L
authors.initials: CL
authors.lastname: Smith
authors.forename: I
authors.initials: I
authors.lastname: Felix
authors.forename: S
authors.initials: S
authors.lastname: Kumar
authors.forename: S
authors.initials: S
authors.lastname: Nampalli
mesh_terms: D001483:Base Sequence; D018360:Crystallography, X-Ray; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D009711:Nucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment
chemical_list: D009711:Nucleotides; D011758:Pyrroles; D004259:DNA-Directed DNA Polymerase