title: Maternal expression of functional lipoprotein lipase and effects on body fat mass and body condition scores of mature cats with lipoprotein lipase deficiency.
abstract: OBJECTIVE
  To assess effects of deficiency of lipoprotein lipase (LPL) on body condition scores and lean and fat body masses of adult cats.
  
  
  METHODS
  12 cats without LPL mutations and 23 cats that were heterozygous or homozygous carriers of the Gly412Arg LPL mutation.
  
  
  METHODS
  Lean and fat body masses were estimated by use of body condition scores and change in enrichment of serum after IV administration of deuterium oxide. Mass spectroscopy and infrared absorbance methods were used to determine deuterium enrichment.
  
  
  RESULTS
  Fat body mass (mean +/- SD; 0.2 +/- 0.1 kg) and percentage body fat (6.2 +/- 1.4%) of homozygotes were significantly less than those of clinically normal cats and heterozygotes (0.7 +/- 0.1 kg, 18.2 +/- 1.6% and 0.5 +/- 0.1 kg, 15.6 +/- 1.7%, respectively). Homozygous offspring of homozygous dams had significantly less fat body mass (0.1 +/- 0.1 kg) and percentage body fat (2.1 +/- 1.0%) than homozygous offspring of heterozygous dams (0.3 +/- 0.1 kg and 9.2 +/- 1.7%, respectively). Lean body mass did not differ significantly among groups. For all groups, percentage body fat was significantly correlated with body condition score (r= 0.65), and body condition scores supported findings for fat body mass.
  
  
  CONCLUSIONS
  Deficiency of LPL activity in cats diminishes stores of body fat. This is consistent with a low rate of de novo synthesis of fat. The effect of dam on body masses in mature LPL-deficient cats indicates nutrient programming of adipose formation during gestation or lactation.
authors.affiliation: Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis 95616, USA.
authors.forename: R C
authors.initials: RC
authors.lastname: Backus
authors.forename: D G
authors.initials: DG
authors.lastname: Ginzinger
authors.forename: K J
authors.initials: KJ
authors.lastname: Ashbourne Excoffon
authors.forename: S M
authors.initials: SM
authors.lastname: Clee
authors.forename: M R
authors.initials: MR
authors.lastname: Hayden
authors.forename: R H
authors.initials: RH
authors.lastname: Eckel
authors.forename: M A
authors.initials: MA
authors.lastname: Hickman
authors.forename: Q R
authors.initials: QR
authors.lastname: Rogers
mesh_terms: D000273:Adipose Tissue / Q000201:enzymology*; D000818:Animals; D000831:Animals, Newborn; D001823:Body Composition / Q000235:genetics; D002371:Cat Diseases / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D002415:Cats; D017666:Deuterium Oxide; D005260:Female; D015870:Gene Expression; D005838:Genotype; D008071:Lipoprotein Lipase / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism; D008297:Male
chemical_list: D008071:Lipoprotein Lipase; D017666:Deuterium Oxide