title: Synthesis and properties of 2'-deoxycytidine triphosphate carrying c-myc tag sequence.
abstract: The synthesis of 2'-deoxycytidine triphosphate carrying mercaptoethyl groups at position 4 of cytosine is described. This nucleoside triphosphate was reacted with a maleimido-peptide carrying the c-myc tag-sequence to yield a peptide-nucleoside triphosphate chimera. Primer extension studies showed that the nucleoside triphosphate modified with the peptide sequence is incorporated by DNA polymerases opposite guanine.
authors.affiliation: Universität Ulm, Sektion Polymere, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Hinz
authors.forename: D
authors.initials: D
authors.lastname: Gottschling
authors.forename: R
authors.initials: R
authors.lastname: Eritja
authors.forename: H
authors.initials: H
authors.lastname: Seliger
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D003845:Deoxycytosine Nucleotides / Q000138:chemical synthesis* / Q000737:chemistry* / Q000378:metabolism; D004795:Enzyme Stability; D016259:Genes, myc
chemical_list: D017931:DNA Primers; D003845:Deoxycytosine Nucleotides; C024107:2'-deoxycytidine 5'-triphosphate; D004259:DNA-Directed DNA Polymerase