Cytochrome P450-2E1 and glutathione S-transferase mu polymorphisms among Caucasians and mulattoes from Brazil.
The variable interindividual ability to metabolize environmental toxicants, also known as metabolic polymorphism, may be of substantial importance in the modulation of cancer risk. The ethnic distribution of these polymorphisms could be interesting in order to establish an association with cancer risk or even to establish selective advantage of some genotypes. Cytochrome P450 2E1 (CYP2E1) is a secondary enzyme that can metabolize ethanol, and glutathione S-transferase (GSTM1) is thought to be involved in the detoxification of epoxides and polycyclic aromatic hydrocarbons. Mutation in these genes was investigated in a random sample of healthy subjects from São Paulo, Brazil, which included 206 Caucasians and 86 mulattoes. Pst I restriction fragment length polymorphism (RFLP) in the 5'-flanking region of the CYP2E1 gene has been identified in 10.2% of Caucasian individuals and in 11.6% of mulattoes. For GSTM1 the frequency of the null genotype was significantly higher in Caucasian individuals (60.2%) than in mulattoes (41.9%). Allele frequencies were (1) CYP2E1 locus: P = 0.949, q = 0.051, se(p) = se(q) = 0.011 among Caucasians; and p = 0.942; q = 0.058; se(P) = se'(q) = 0.018 among mulattoes; and (2) GSTM1 locus: p = 0.224, q = 0.776, se(p) = se(q) = 0.022 among Caucasians; and p = 0.353; q = 0.647; se(p) = se(q) = 0.041 among mulattoes.
Gattás
G J
GJ
Department of Legal Medicine, Ethics and Occupational Health, Medical School, University of São Paulo, Brazil. gfgattas@usp.br
Soares-Vieira
J A
JA
D000328:Adult; D044383:Black People; D001938:Brazil / Q000208:ethnology; D002274:Carcinogens, Environmental / Q000378:metabolism; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D008297:Male; D009369:Neoplasms / Q000235:genetics*; D011110:Polymorphism, Genetic; D044465:White People
D002274:Carcinogens, Environmental; D019392:Cytochrome P-450 CYP2E1; D005982:Glutathione Transferase