Human lung fructose-1,6-bisphosphatase is localized in pneumocytes II.
The localization of fructose-1,6-bisphosphatase (Fru-1,6-Pase EC 3.1.3.11) in human alveolar epithelium was determined immunohistochemically using a polyclonal antibody raised against the enzyme purified from human liver. The immunohistochemical analysis revealed that the Fru-1,6-Pase was localized in pneumocytes II and was absent in pneumocytes I. Hypothetically Fru-1,6-Pase participating in glucose-6-phosphate synthesis from noncarbohydrate precursors increases NADPH level which is used for surfactant synthesis and for glutathione redox cycle.
Gizak
A
A
Department of Animal Physiology, Institute of Zoology, Wroclaw University, Poland.
Rakus
D
D
Kolodziej
J
J
Zabel
M
M
Ogorzalek
A
A
Dzugaj
A
A
D000818:Animals; D006597:Fructose-Bisphosphatase / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008168:Lung / Q000166:cytology* / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D016612:Paraffin Embedding; D011650:Pulmonary Alveoli / Q000166:cytology / Q000201:enzymology; D011817:Rabbits / Q000276:immunology; D016707:Tissue Fixation
D006597:Fructose-Bisphosphatase