title: Characterization of the MHC class I region of the Japanese pufferfish (Fugu rubripes).
abstract: A BAC map of the Japanese pufferfish (Fugu) MHC class I region was constructed using a mixture of sequence scanning and sequence-tagged site mapping methodologies. The Fugu MHC class Ia genes are linked to genes which are found within the human classical MHC class II and extended class II regions, a situation which has been found in the MHC of all teleosts mapped so far. The 300-kb contig comprises 24 MHC-related genes and is bounded by six non-MHC genes, which are thought to represent an evolutionary breakpoint within the region. Comparative analysis with both human and zebrafish MHC maps indicates two blocks of genes (KNSL2, ZNF297, DAXX, TAPBP, FLOTILLIN; and PSMB8, PSMB10, PSMB9, ABCB3, FABGL, BRD2, COL11A2, RXRB) which have remained linked over 400 million years and may represent an ancestral arrangement of the vertebrate MHC. Zebrafish and Fugu diverged between 100-200 million years ago and differences exist between these two fish species. The position and number of MHC class Ia genes is not conserved between species, there is an inversion of a block of nine genes centering on the PSMB cluster, and additional genes are present in zebrafish coding for a transport-associated protein and a beta proteasome subunit. The extent of these differences has implications for the extrapolation of fish model organism data to commercial aquaculture species. The data presented here represent the most extensive analysis of a fish MHC class Ia region described so far and clearly delimit the extent of this region in Fugu and, potentially, all teleosts.
authors.affiliation: Fugu Genomics, HGMP Resource Centre,  Hinxton, Cambridge, UK. mclark@hgmp.mrc.ac.uk
authors.forename: M S
authors.initials: MS
authors.lastname: Clark
authors.forename: L
authors.initials: L
authors.lastname: Shaw
authors.forename: A
authors.initials: A
authors.lastname: Kelly
authors.forename: P
authors.initials: P
authors.lastname: Snell
authors.forename: G
authors.initials: G
authors.lastname: Elgar
mesh_terms: D000818:Animals; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics; D020451:Contig Mapping; D019143:Evolution, Molecular; D005399:Fishes / Q000235:genetics* / Q000276:immunology*; D008040:Genetic Linkage / Q000235:genetics; D015698:Genomic Library; D015395:Histocompatibility Antigens Class I / Q000235:genetics* / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology; D006801:Humans; D007564:Japan; D008285:Major Histocompatibility Complex / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016324:Sequence Tagged Sites; D009626:Terminology as Topic
chemical_list: D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II