The effects of propofol on NMDA- or nitric oxide-mediated neurotoxicity in vitro.
Acute brain ischemia causes neurotoxic cascades including NMDA receptors and NO. Propofol, an i.v. anesthetic, is thought to have a neuroprotective effect. We investigated the influence of propofol on NMDA/NO neurotoxicity using Shibuta's established model of primary brain cultures. Cortical neurons prepared from E16 were used after 13-14 days in culture. The neurons were exposed to various concentrations of propofol with NMDA or NO-donor. The survival rates of neurons exposed to 30 microM NMDA with or without 300 microM propofol were 12.1 +/- 2.2% and 11.9 +/- 2.2%, respectively. The survival rates exposed to 30 microM NO-donor with or without 300 microM propofol were 11.2 +/- 4.2% and 14.0 +/- 3.9%, respectively. These results suggest that neuroprotective effect of propofol is limited and propofol does not offer advantages over thiopental against NMDA/NO-induced cytotoxicity.
Shibuta
S
S
Department of Anesthesiology, Osaka University Medical School, Suita-city, Japan.
Sriranganathan
V
V
Inoue
T
T
Shimizu
T
T
Tomi
K
K
Mashimo
T
T
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D018690:Excitatory Amino Acid Agonists / Q000633:toxicity*; D005260:Female; D005333:Fetus / Q000166:cytology; D016166:Free Radical Scavengers / Q000494:pharmacology*; D066298:In Vitro Techniques; D016202:N-Methylaspartate / Q000633:toxicity*; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D011247:Pregnancy; D015742:Propofol / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D014226:Triazenes / Q000494:pharmacology
C114535:1-hydroxy-2-oxo-3-(3-aminopropyl)-3-isopropyl-1-triazene; D018690:Excitatory Amino Acid Agonists; D016166:Free Radical Scavengers; D020030:Nitric Oxide Donors; D014226:Triazenes; D009569:Nitric Oxide; D016202:N-Methylaspartate; D015742:Propofol