A method for large-scale multiplication of Curculigo orchioides through bulbil formation from leaf explant in shake flask culture.
An efficient method has been developed for large-scale multiplication of Curculigo orchioides (Hypoxidaceae), an endangered medicinal plant, through direct bulbil formation from leaf explants in shake flask cultures. Leaf-segments (7 x 10 mm) were cultured in B5 liquid medium containing KNO3 (200 mgNL-1), (NH4)2SO4 (50 mgNL-1), benzyl adenosine (2.2 microM), adenine (0.11 mM), indole butyric acid (1.0 microM) and polyvinyl pyrrolidone (250 mgL-1). About 95% of explants produced maximum number of bulbils (546/flask at 6 weeks growth) in the medium. Shake flask cultures yielded 2737 bulbils/L medium whereas static cultures yielded 624 bulbils/L medium. Germination of bulbils was maximum (90.62%) on agar-gelled B5 medium containing benzyl adenosine (2.2 microM) and gibberellic acid (3.5 microM). Plantlets developed in vitro were successfully transferred to soil with a high rate of survivability (90%) and were comparable to natural population in growth and vigour.
Suri
S S
SS
Laboratory of Bio-Molecular Technology, Department of Botany, M L Sukhadia University, Udaipur 313 001, India.
Arora
D K
DK
Ramawat
K G
KG
D001901:Botany / Q000379:methods; D018525:Germination; D019684:Magnoliopsida / Q000254:growth & development*; D018515:Plant Leaves / Q000254:growth & development; D010946:Plants, Medicinal / Q000254:growth & development*