title: [A study of reduction of NF 2 protein, mRNA and loss of 22 q in sporadic meningiomas].
abstract: Neurofibromatosis 2(NF 2) is one of the tumor suppressor genes, and its disorder has been reported in sporadic meningiomas. We investigated some reduction of expression of NF 2 protein and mRNA in 33 sporadic meningiomas, using immunohistochemistry and in situ hybridization, respectively. Seventeen of 33 (51.5%) meningiomas demonstrated significantly reduced or absent NF 2 protein expression. Its mRNA was also reduced or absent in 11 of 24(45.8%) meningiomas, and some statistically significant correlation was shown between reductions of NF 2 protein and its mRNA. Furthermore, we studied about loss of 22 q in 5 meningiomas with fluorescence in situ hybridization. In the cases with reduction of NF 2 protein and/or mRNA, 22 q telomere signals were observed as loss of heterozygosity. On the other hand, in the cases with expression of NF 2 protein and mRNA, 22 q telomere signals were normal. In conclusion, reduction or absence of NF 2 protein and mRNA may play an important role in the tumorigenesis of about half number of sporadic meningiomas.
authors.affiliation: Department of Pathology, Tokyo Medical Dental University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Ikeda
authors.forename: H
authors.initials: H
authors.lastname: Kimura
authors.forename: E
authors.initials: E
authors.lastname: Honma
authors.forename: T
authors.initials: T
authors.lastname: Shibata
authors.forename: S
authors.initials: S
authors.lastname: Yamazaki
authors.forename: K
authors.initials: K
authors.lastname: Hashimoto
authors.forename: Y
authors.initials: Y
authors.lastname: Eishi
mesh_terms: D002892:Chromosomes, Human, Pair 22 / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D019656:Loss of Heterozygosity; D008565:Membrane Proteins / Q000235:genetics*; D008579:Meningioma / Q000235:genetics*; D025581:Neurofibromin 2; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics
chemical_list: D008565:Membrane Proteins; D025581:Neurofibromin 2; D012333:RNA, Messenger