title: Effects of exposure of lactating female rats to polychlorinated biphenyls (Pcbs) on testis weight, sperm production and sertoli cell numbers in the adult male offspring.
abstract: To investigate the effects of intermittent and continuous exposure of lactating rats to Aroclor 1242 (a PCB congener), testis weight, daily sperm production (DSP) and Sertoli cell number per testis were examined in the adult male offspring. Thyroxine (T4) was also measured because of the well-documented effects of polychlorinated biphenyls (PCBs) on this hormone. In experiment 1.3 groups of lactating female rats received daily subcutaneous injections of low (0.8 mg) or high (1.6 mg) doses of Aroclor 1242 in 0.1 ml corn oil from parturition to weaning of pups at 21 days. In experiment II, 3 groups of lactating rats received 2 subcutaneous injections per week of 0.8 or 1.6 mg Aroclor 1242, as in experiment 1. In both experiments, control rats received vehicle alone. Serum T4 was measured at 21 and 90 days of age, and testis weight, DSP and Sertoli cell numbers were examined at 90 days. In experiment I (continuous exposure), both the low (0.8 mg) and high (1.6 mg) doses suppressed T4 concentrations at 21 days of age. Testis weight was increased by 14.8% (LD) and 16.5% (HD) compared with controls. DSP was increased by 20.4% in the low dose and 25% in the high dose animals compared with controls. The number of Sertoli cells per testis was increased by 32.6 and 39.4% in low and high dose animals, respectively. A similar study in which the lactating females were only dosed twice per week (experiment 11) did not show any differences in these parameters. These results indicate that continuous exposure of lactating female rats to PCBs increases testis weight, sperm production and Sertoli cell numbers in the adult male offspring.
authors.affiliation: Department of Anatomy, College of Veterinary Medicine, Chonbuk National University, Chonju, Republic of Korea.
authors.forename: I S
authors.initials: IS
authors.lastname: Kim
mesh_terms: D000375:Aging; D000818:Animals; D001140:Aroclors / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D004785:Environmental Pollutants / Q000494:pharmacology*; D005260:Female; D007774:Lactation; D008297:Male; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D012708:Sertoli Cells / Q000166:cytology / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects / Q000502:physiology*; D013737:Testis / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development; D013974:Thyroxine / Q000097:blood
chemical_list: D001140:Aroclors; D004785:Environmental Pollutants; C027423:aroclor 1242; D013974:Thyroxine