Study of cell-material interaction by estimating NF-kappaB activation in HeLa S3 cells adhered onto hydrophilic substrates.
In order to interpret how cells recognize biomaterials, nucleic factor-kappa B (NF-kappaB) activation in the attached HeLa S3 cells on various substrates was evaluated. As substrates, materials of hydrophilic nature (cellulose, poly(acrylamide)-grafted poly(ethylene) (PAAm-g-PE), and lipids films) were used. The contemporary assay method for NF-kappaB was modified to fit our system. As a result, NF-kappaB activation varied depending on the substrates. The NF-kappaB outcome was induced significantly in the HeLa S3 cells that had adhered onto the lipid films in a short time. On the other hand, high levels of NF-kappaB induction were observed in the HeLa cells adhered to the celluose and PAAm-g-PE after a 24 h incubation period. The induction of NF-kappaB by cell-material interaction is discussed from the point of view of biocompatibility.
Kishida
A
A
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, Korimoto, Japan. kishida@ri.ncvc.go.jp
Matsuyama
T
T
Kitajima
I
I
Maruyama
I
I
Akashi
M
M
D002448:Cell Adhesion; D006367:HeLa Cells; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism*
D016328:NF-kappa B