title: The influence of the phase of the estrous cycle on sheep endometrial tissue response to lipopolysaccharide.
abstract: The effect of lipopolysaccharide on ovine endometrial tissue was examined at estrus (follicular phase) and during the luteal phase. Endometrial tissues were cultured with 0, 1, or 10 microg/mL lipopolysaccharide. After 24 h, culture supernates were harvested and analyzed for PGF2alpha, PGE2, 6-keto-PGF1alpha, thromboxane B2 (TXB2), and cysteinyl-leukotrienes (leukotrienes) using EIA. Homogenates of endometrial tissue were analyzed for prostaglandin endoperoxidase-1 (PTGS-1), and -2 (PTGS-2) as well as Type-I, -II, and -III nitric oxide synthase (NOS) by Western analysis. Follicular phase tissue produced more PGF2alpha (P < 0.001), TXB2 (P < 0.001), and leukotrienes (P < 0.02) than luteal tissue. Lipopolysaccharide increased PGE2 (P < 0.001) and TXB2 (P < 0.02) production by endometrial tissue. Elevations in these eicosanoids were likely due to the measured increases in PTGS-2 (P = 0.002) because no changes in PTGS-1 (P = 0.54) or Type-I, -II, or -III NOS (P > or = 0.20) occurred in endometrial tissue following lipopolysaccharide exposure. These data suggest that the phase of the estrous cycle regulates prostaglandin production by immune-challenged endometrial tissue.
authors.affiliation: Department of Animal, Dairy, and Veterinary Sciences, Utah State University, Logan 84322, USA. kvagnoni@sherbtel.net
authors.forename: K E
authors.initials: KE
authors.lastname: Vagnoni
authors.forename: S B
authors.initials: SB
authors.lastname: Abbruzzese
authors.forename: N D
authors.initials: ND
authors.lastname: Christiansen
authors.forename: G R
authors.initials: GR
authors.lastname: Holyoak
mesh_terms: D015121:6-Ketoprostaglandin F1 alpha / Q000032:analysis / Q000096:biosynthesis; D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D015237:Dinoprost / Q000032:analysis / Q000096:biosynthesis / Q000494:pharmacology; D015232:Dinoprostone / Q000032:analysis / Q000096:biosynthesis; D004717:Endometrium / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology*; D004971:Estrus / Q000502:physiology*; D004973:Estrus Synchronization / Q000187:drug effects; D005260:Female; D007091:Image Processing, Computer-Assisted; D015289:Leukotrienes / Q000032:analysis / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000494:pharmacology*; D019001:Nitric Oxide Synthase / Q000032:analysis; D052248:Nitric Oxide Synthase Type I; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; D011451:Prostaglandin-Endoperoxide Synthases / Q000032:analysis; D012756:Sheep / Q000502:physiology*; D013929:Thromboxane B2 / Q000032:analysis / Q000096:biosynthesis
chemical_list: D015289:Leukotrienes; D008070:Lipopolysaccharides; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D015237:Dinoprost; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; D011451:Prostaglandin-Endoperoxide Synthases; D015232:Dinoprostone