The rat beta (b miny)-globin promoter: nuclear protein factors and erythroid-specific induction of transcription.
We show that the rat adult beta (b miny)-globin gene is transcriptionally active. The - 100-bp promoter region contains control elements that are important for the induction of transcription in rat erythroleukemia (REL) cells. By using DNaseI footprinting and gel mobility shift assays, we have shown that the CCAAT box, a regulatory element from an analyzed promoter region, binds NF-Y and GATA-1 transcription factors. Another regulatory element from this region, betaDRE, binds erythroid-specific and ubiquitous factors from REL cells. Although both the CCAAT box region and betaDRE element bind the same protein factors before and after induction of REL cells, we show by South-Western blot analysis that the concentrations of 150-kDa, 70-kDa and 60-kDa factors binding to the betaDRE are increased in DMSO-induced REL cells.
Pavlović
S
S
Laboratory for Molecular Hematology, Institute of Molecular Genetics and Genetic Engineering, Belgrade, Yugoslavia. sonya@sezampro.yu
Mitrović
T
T
Nikcević
G
G
Grujicić
N
N
Lazić
D
D
Glisin
V
V
Popović
Z
Z
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics / Q000378:metabolism; D018983:DNA Footprinting; D004268:DNA-Binding Proteins / Q000378:metabolism; D003850:Deoxyribonuclease I / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism*; D017930:Genes, Reporter / Q000235:genetics; D005914:Globins / Q000145:classification / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D051379:Mice; D009687:Nuclear Proteins / Q000378:metabolism*; D009928:Organ Specificity; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D020218:Response Elements / Q000235:genetics; D012156:Reticulocytes / Q000378:metabolism; D015534:Trans-Activators / Q000378:metabolism; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D014407:Tumor Cells, Cultured
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D015534:Trans-Activators; D005914:Globins; D004247:DNA; D003850:Deoxyribonuclease I