title: Lactate dehydrogenase leakage of hepatocytes with AH26 and AH Plus sealer treatments.
abstract: Numerous root canals filling materials are available in the field of dentistry, based on various formulas that contain a variety of different and partly mutagenic components, such as epoxy resin sealers, Ca(OH)2-based materials, and zinc oxide-eugenol cements. AH Plus root canal sealer will not release formaldehyde according to the manufacturer, although AH26 does. The purpose of this study was to analyze the leakage of lactate dehydrogenase (LDH) from rat hepatocytes after treatment with AH26 and AH Plus root canal sealers in vitro. Hepatocytes from male Sprague-Dawley rats were used to test the cytotoxicity of AH26 and AH Plus. The root canal sealers were mixed and then dissolved in the dimethyl sulfoxide to final concentrations of 0.01%, 0.04%, and 0.1% (wt/vol), with a dimethyl sulfoxide concentration of < 0.05%. Dosage-dependent and time-dependent lactate dehydrogenase leakage values were measured and tested by one-way ANOVA. The results showed that both AH26 and AH Plus are toxic to rat hepatocytes. At a low concentration, AH26 had a higher toxicity than AH Plus to rat hepatocytes.
authors.affiliation: Dental Department, Chung Shan Medical and Dental College, Taichung, Taiwan, Republic of China.
authors.forename: T H
authors.initials: TH
authors.lastname: Huang
authors.forename: C K
authors.initials: CK
authors.lastname: Lii
authors.forename: M Y
authors.initials: MY
authors.lastname: Chou
authors.forename: C T
authors.initials: CT
authors.lastname: Kao
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001729:Bismuth / Q000633:toxicity*; D016923:Cell Death; D002478:Cells, Cultured / Q000187:drug effects / Q000201:enzymology; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004853:Epoxy Resins / Q000633:toxicity*; D022781:Hepatocytes / Q000187:drug effects* / Q000201:enzymology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D008709:Methenamine / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012387:Root Canal Filling Materials / Q000633:toxicity*; D012834:Silver / Q000633:toxicity*; D013997:Time Factors; D014025:Titanium / Q000633:toxicity*; D018675:Toxicity Tests
chemical_list: D004338:Drug Combinations; D004853:Epoxy Resins; D012387:Root Canal Filling Materials; C534916:epoxy resin-based root canal sealer; D012834:Silver; C028044:epoxy resin AH-26; D014025:Titanium; D007770:L-Lactate Dehydrogenase; D008709:Methenamine; D001729:Bismuth