A histopathological and immunohistochemical study of dental pulp and pulpal nerve fibers in rats after the cavity preparation using Er:YAG laser.
The purpose of this study was to investigate histopathological changes in the dental pulp after Er:YAG laser irradiation compared with those after high-speed drill preparation. For evaluation, repair of nerve fibers was observed using an immunohistochemical technique. There was no significant difference between the remaining dentin thickness in either cases. (Mann-Whitney U test). In the Er:YAG laser group a marked fibroblast proliferation and the formation of reparative dentin were observed relative to the high-speed drill group. The time course of the increase and decrease in calcitonin gene-related peptide-immunoreactive fibers in the high-speed drill group was similar to that of previous reports. In the Er:YAG laser group an increase in calcitonin gene-related peptide-immunoreactive fibers was seen earlier than in the high-speed drill group, and 7 days after operation these fibers decreased to control level. The results suggested that the Er:YAG laser leads to pulpal repair earlier than the high-speed drill.
Takamori
K
K
Department of Pedodontics, Nihon University School of Dentistry at Matsudo, Matsudo, Japan.
D000538:Aluminum Silicates; D000704:Analysis of Variance; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis; D002455:Cell Division / Q000528:radiation effects; D003737:Dental Cavity Preparation / Q000145:classification / Q000295:instrumentation / Q000379:methods*; D003754:Dental High-Speed Equipment; D003782:Dental Pulp / Q000294:innervation / Q000528:radiation effects*; D003804:Dentin / Q000294:innervation / Q000473:pathology / Q000528:radiation effects; D003809:Dentin, Secondary / Q000473:pathology / Q000528:radiation effects; D004871:Erbium; D005347:Fibroblasts / Q000473:pathology / Q000528:radiation effects; D007150:Immunohistochemistry; D053685:Laser Therapy; D008297:Male; D009412:Nerve Fibers / Q000528:radiation effects* / Q000648:ultrastructure; D020078:Neurogenic Inflammation / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D018709:Statistics, Nonparametric; D013997:Time Factors; D014945:Wound Healing; D015019:Yttrium
D000538:Aluminum Silicates; D015019:Yttrium; D004871:Erbium; D015740:Calcitonin Gene-Related Peptide