Physicochemical characterisation of the pertussis vaccine.
The characterisation of an acellular pertussis vaccine composed of a genetically modified pertussis toxin, filamentous haemagglutinin and pertactin is described. The three antigens are submitted to a mild treatment with formaldehyde in the presence of lysine before their use in vaccine formulation. Characterisation is performed by amino acid analysis, SDS-PAGE, analytical size exclusion chromatography and, in the case of pertactin, isoelectrofocusing. The effect of some variables on pertactin formaldehyde treatment has been studied by means of isoelectrofocusing and mouse immunogenicity.
Ceccarini
C
C
Chiron Vaccines, Siena, Italy
Contorni
M
M
Costantino
P
P
D'Ascenzi
S
S
Gallo
E
E
Maffei
M
M
Mannucci
D
D
Marsili
I
I
Magagnoli
C
C
Peppoloni
S
S
Rappuoli
R
R
Ravenscroft
N
N
Ricci
S
S
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D015153:Blotting, Western; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005557:Formaldehyde / Q000737:chemistry; D007525:Isoelectric Focusing; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D037342:Pertussis Toxin; D010567:Pertussis Vaccine / Q000737:chemistry* / Q000276:immunology; D010566:Virulence Factors, Bordetella / Q000737:chemistry / Q000235:genetics / Q000276:immunology
D000596:Amino Acids; D010567:Pertussis Vaccine; D010566:Virulence Factors, Bordetella; D005557:Formaldehyde; D037342:Pertussis Toxin