title: [Both alpha1- and beta-adrenergic receptors participate in generation of a Ca2+-response to noradrenaline in murine brown preadipocytes].
abstract: It has been shown that in Ca(2+)-signal generation, at the addition of norepinephrine (NE) to a suspension of freshly isolated brown preadipocytes, beta-receptors participate alongside with alpha 1-adrenoreceptors. The amplitude of cell response to 10(-5)-10(-8) M NE is approximately equal to the sum of effects of alpha 1-specific agonists (oxymetazoline or cirazoline) and beta-selective agonist isoproterenol. beta-Selective antagonist nadolol practically completely prevented the effect of NE on the intracellular Ca2+ level, while phentolamine, an antagonist of alpha-receptors, caused only a approximately 25% inhibition of the cellular response.
authors.affiliation: Institute of Cell Biophysics RAS, Pushchino, Moscow region.
authors.forename: L P
authors.initials: LP
authors.lastname: Dolgacheva
authors.forename: E N
authors.initials: EN
authors.lastname: Galitovskaia
authors.forename: A S
authors.initials: AS
authors.lastname: Baumuratov
authors.forename: V P
authors.initials: VP
authors.lastname: Zinchenko
authors.forename: G E
authors.initials: GE
authors.lastname: Bronnikov
mesh_terms: D017667:Adipocytes / Q000187:drug effects / Q000502:physiology*; D002001:Adipose Tissue, Brown / Q000187:drug effects / Q000502:physiology*; D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000502:physiology*; D008297:Male; D051379:Mice; D009638:Norepinephrine / Q000502:physiology*; D018340:Receptors, Adrenergic, alpha-1 / Q000502:physiology*; D011943:Receptors, Adrenergic, beta / Q000502:physiology*
chemical_list: D000317:Adrenergic alpha-Antagonists; D000319:Adrenergic beta-Antagonists; D018340:Receptors, Adrenergic, alpha-1; D011943:Receptors, Adrenergic, beta; D002118:Calcium; D009638:Norepinephrine
vernacular_title: V generatsii Ca2+-otveta na noradrenalin v burykh preadipotsitakh myshi uchastvuiut kak alpha1-, tak i beta-adrenoretseptory.