In utero radiation-induced apoptosis and p53 gene expression in the developing rat brain.
OBJECTIVE
This study addressed the question of the role of the p53 gene in prenatal low-dose radiation-induced apoptosis in the neuroepithelium, in an effort to elucidate molecular mechanisms involved in the extreme radiosensitivity of the developing brain.


METHODS
Pregnant Wistar rats were exposed to a single dose of 10, 20 or 40 cGy of X-rays on day 15 or 17 of gestation. Animals were sacrificed 4 or 24h after exposure. Apoptosis was studied by gel electrophoresis of isolated DNA and in situ by the TUNEL reaction. Expression of the p53 gene was studied by immunocytochemistry and Western analysis, as well as Northern analysis, for the detection of the protein and mRNA respectively.


RESULTS
In utero low-dose irradiation led to apoptosis and an increase of p53 gene expression in the developing rat brain. Apoptotic as well as p53 immunopositive cells were detected among proliferating, migratory and post-mitotic neurones in the developing neuroepithelium following prenatal irradiation, even after only l0 cGy. In addition to the p53 protein, p53 mRNA brain levels were also increased following prenatal irradiation.


CONCLUSIONS
Low-dose prenatal irradiation of the developing brain led to p53 induction and cell death by apoptosis.
Bolaris
S
S
Laboratory of Biology-Biochemistry, Faculty of Nursing, University of Athens, Greece.
Bozas
E
E
Benekou
A
A
Philippidis
H
H
Stylianopoulou
F
F
D000818:Animals; D017209:Apoptosis / Q000528:radiation effects*; D015152:Blotting, Northern; D015153:Blotting, Western; D001921:Brain / Q000196:embryology* / Q000378:metabolism / Q000528:radiation effects*; D002453:Cell Cycle / Q000528:radiation effects; D053938:DNA Fragmentation / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D004622:Embryo, Mammalian / Q000528:radiation effects*; D004848:Epithelium / Q000196:embryology; D005260:Female; D016158:Genes, p53 / Q000528:radiation effects*; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008856:Microscopy, Fluorescence; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000528:radiation effects*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis / Q000528:radiation effects*
D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53