Calcium inhibits willardiine-induced responses in kainate receptor GluR6(Q)/KA-2.
A number of studies have demonstrated that willardiine [(S)-1-(2-amino-2-carboxyethyl) pyrimidine-2,4-dione] is a useful agonist for the activation of AMPA/kainate receptors. Here we examine the effect of extracellular calcium on currents evoked by willardiine in HEK 293 cells expressing the GluR6(Q)/KA-2 kainate receptor subunits. At a concentration of 1.8 mM, Ca2+ inhibited the currents induced by 100 microM willardiine by approximately 50%. When extracellular Na+ ions were replaced with Ca2+ ions there were no measurable inward currents. We conclude that Ca2+ inhibition of the willardiine-induced response is concentration dependent.
Fukushima
T
T
Department of Information Science, Iwate University, Morioka Japan.
Marshall
J
J
Sakata
K
K
Shingai
R
R
D000409:Alanine / Q000031:analogs & derivatives / Q000494:pharmacology*; D002122:Calcium Chloride / Q000494:pharmacology*; D002478:Cells, Cultured / Q000187:drug effects; D006801:Humans; D007668:Kidney; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D011744:Pyrimidinones; D018092:Kainic Acid Receptors / Q000187:drug effects* / Q000502:physiology; D014498:Uracil; D000097806:GluK2 Kainate Receptor
D000409:Alanine; D002122:Calcium Chloride; D011744:Pyrimidinones; D018092:Kainic Acid Receptors; D014498:Uracil; D000097806:GluK2 Kainate Receptor; C002872:willardiine