title: The complete nucleotide sequences of L3 and S7 segments of Ibaraki virus encoding for the major inner capsid proteins, VP3 and VP7.
abstract: The complete nucleotide sequences of the genes encoding two of the major inner capsid proteins of Ibaraki virus (IBAV), belonging to epizootic hemorrhagic disease virus serotype 2 (EHDV-2) were determined. The L3 RNA segment is 2768 nucleotides in length which encodes VP3 polypeptides of 899 amino acid residues (M.W. 103 kDa). The S7 RNA segment, which encodes the VP7 core protein, is 1162 nucleotides in length and encodes 349 amino acids (M.W. 38 kDa). These RNA segments had the characteristic consensus motifs of Orbivirus RNA segments in termini, namely 5'-GUUAAA... and ...ACUUAC-3'. The comparison of the IBAV L3 and S7 sequences with those of other two EHDV-2 isolates revealed the higher homologies of 93% and 92% against EHDV-2 Australia isolate (EHDV-2AUS) and lower homologies of 80% and 81% against EHDV-2 North America isolate, respectively. The phylogenetic analysis based on L3 and S7 genes also indicated close relationships between IBAV and EHDV-2AUS.
authors.affiliation: Department of Veterinary Hygiene, Faculty of Agriculture, Yamaguchi University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Iwata
authors.forename: S
authors.initials: S
authors.lastname: Manabe
authors.forename: A
authors.initials: A
authors.lastname: Yoshida
authors.forename: E M
authors.initials: EM
authors.lastname: Pereira
authors.forename: T
authors.initials: T
authors.lastname: Inoue
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002213:Capsid / Q000737:chemistry*; D036022:Capsid Proteins; D002460:Cell Line; D019143:Evolution, Molecular; D018164:Hemorrhagic Disease Virus, Epizootic / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D012703:Serotyping; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics*
chemical_list: D036022:Capsid Proteins; D012367:RNA, Viral; C095283:VP3 protein, Epizootic hemorrhagic disease virus 2; C427245:VP7 protein, epizootic hemorrhagic disease virus-2; D014764:Viral Proteins