Changes in lung-specific molecular expression during differentiation of hamster embryonic M3E3/C3 cell line.
To clarify the differentiation mechanisms of bronchiolar epithelial cells, changes at the transcription level of epithelial cell-specific proteins were examined using M3E3/C3, a cell line derived from hamster fetal lung. During a 9-day incubation period with 24 microg/mL of retinol, the cells became attached to each other and formed large bud-like structures which could be detected with periodic acid-Schiff staining. During the incubation period, the mRNA level of surfactant-associated protein-B significantly increased 2.6- and 5.4-fold higher than cells incubated without retinol on days 3 and 9, respectively. The Clara cell-specific secretory protein mRNA level also increased and peaked at 5.1-fold (P < 0.05) on day 5 compared with control cells. In contrast, mRNA for surfactant-associated protein-C, an alveolar type II cell-specific protein, decreased. Moreover, the expression of the gene for hepatocyte nuclear factor 3alpha, a putative transactivating factor for lung-related genes, was up-regulated resulting in consistently higher levels (2.4- to 5.6-fold) compared with controls, while those for transmembrane-type mucin-1 and glyceraldehyde-3-phosphate dehydrogenase were constantly expressed during the incubation. The present study confirms that at the gene transcription level M3E3/C3 cells differentiate into Clara-like cells with mucus granules in the presence of retinol.
Yoshimi
T
T
Department of Environmental Molecular Physiology, School of Life Sciences,Tokyo University of Pharmacy and Life Sciences, Hachioji, Japan. yocchan@ls.toyaku.ac.jp
Takahashi
Y
Y
Takahashi
S
S
Miura
T
T
D000818:Animals; D001980:Bronchi / Q000166:cytology* / Q000378:metabolism*; D002454:Cell Differentiation; D002460:Cell Line; D006224:Cricetinae; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D004268:DNA-Binding Proteins / Q000235:genetics; D004622:Embryo, Mammalian; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D051541:Hepatocyte Nuclear Factor 3-alpha; D008647:Mesocricetus; D009687:Nuclear Proteins / Q000235:genetics; D011506:Proteins / Q000235:genetics; D011510:Proteolipids / Q000235:genetics; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020545:Respiratory Mucosa / Q000166:cytology* / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors / Q000235:genetics; D014598:Uteroglobin; D014801:Vitamin A / Q000494:pharmacology
D004268:DNA-Binding Proteins; D051541:Hepatocyte Nuclear Factor 3-alpha; D009687:Nuclear Proteins; D011506:Proteins; D011510:Proteolipids; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants; D012333:RNA, Messenger; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors; D014801:Vitamin A; D014598:Uteroglobin