[Culture of Plasmodium berghei with the short life span in mouse erythrocytes: evaluation of chloroquine resistance in three strains of the malaria parasite].
This comparative study was made to examine chroloquine inhibition of C14-hypoxanthine incorporation (preferably nucleic acid precursor for plasmodium) in the short-living red blood cell cultures in mice infected with P. berghei strains differently sensitive to the agent 24 hours after incubation. These included 1) chloroquine-sensitive H strain; 2) LNK-65 strain having a spontaneously 2-3-fold decreased sensitivity to the agent; 3) strain LNK-65 ChR selected for high resistance to chloroquine. IC50 (chloroquine concentrations that ensure 50% inhibition of the incorporation of nucleic acid precursor into the cells of the parasite) was estimated for 3 P. berghei strains that were differently sensitive to chloroquine. These in vitro values adequately reflect the sensitivity of these strains to the agent in vivo.
Pankova
T G
TG
Maĭer
T V
TV
Igonina
T M
TM
Rabinovich
S A
SA
Morozov
E N
EN
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D002478:Cells, Cultured; D002738:Chloroquine / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004351:Drug Resistance; D004912:Erythrocytes / Q000469:parasitology*; D008136:Longevity; D051379:Mice; D010962:Plasmodium berghei / Q000187:drug effects* / Q000302:isolation & purification
D000962:Antimalarials; D002738:Chloroquine
Korotkozhivushchaia kul'tura Plasmodium berghei v éritrotsitakh mysheĭ: opredelenie ustoĭchivosti k khlorokhinu u trekh shtammov maliariĭnogo parazita.