Acetyl-seryl-aspartyl-lysyl-proline is a novel natural cell cycle regulator of renal cells.
A natural tetrapeptide, acetyl-seryl-aspartyl-lysyl-proline (AcSDKP) is a physiological negative regulator of hematopoiesis. The precursor of AcSDKP, thymocin beta 4, is expressed in many tissues including kidney. The present study examined the antiproliferative effect of AcSDKP in two renal cell lines, namely, renal interstitial fibloblasts cell line (NRK 49F) and renal proximal tubular epitherial cells (LLC-PK1). An addition of AcSDKP for 48 hours in theses cells resulted in a concentration-dependent attenuation in the proliferation rate (significant difference to non-treated cells was observed at 10(-9) to 10(-5) M AcSDKP) determined by a colorimetry of alamer blue oxidation. The cell cycle analysis of NRK 49F cells treated with AcSDKP showed that AcSDKP significantly reduced the ratio of S-phase to G2/M-phases. Thus, physiological concentrations of AcSDKP is capable of altering cell cycle to inhibit the proliferation of renal cells.
Iwamoto
N
N
Department of Pediatric Nephrology, Tokyo Women's Medical University, School of Medicine, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan.
Xano
H J
HJ
Yoshioka
T
T
Shiraga
H
H
Nitta
K
K
Muraki
T
T
Ito
K
K
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D005347:Fibroblasts / Q000187:drug effects; D005355:Fibrosis; D005434:Flow Cytometry; D006131:Growth Inhibitors / Q000494:pharmacology* / Q000633:toxicity; D007668:Kidney / Q000166:cytology* / Q000187:drug effects / Q000473:pathology; D007684:Kidney Tubules / Q000166:cytology / Q000187:drug effects; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D018374:LLC-PK1 Cells; D009842:Oligopeptides / Q000494:pharmacology* / Q000633:toxicity; D013552:Swine
D006131:Growth Inhibitors; D009842:Oligopeptides; D007770:L-Lactate Dehydrogenase; C058504:goralatide