title: Deregulated expression of c-mos in non-small cell lung carcinomas: relationship with p53 status, genomic instability, and tumor kinetics.
abstract: Little is known about the status of the mitogen-activating protein kinase pathways in lung cancer. One of the key molecules taking part in these pathways is the product of the c-mos proto-oncogene, which plays an important role in oocyte maturation. In vitro investigations in somatic cells have shown that c-mos expression has opposing effects on the cell cycle, which suggests that this proto-oncogene may represent an important determinant of aberrant cell function (genomic instability and altered kinetics). A recent study suggests that these effects may be p53 dependent. In view of the apparent link between c-mos and p53, we investigated in a series of 56 non-small cell lung carcinomas: a) the status of c-mos; b) its relationship to genomic instability (aneuploidy) and two kinetic parameters of the tumors, proliferation and apoptotic indexes (AI); and c) its association with p53 alterations and their concomitant relationship with the above parameters. We found c-mos overexpression in 27% of the tumors. Expression was higher in stages II/III (34%) than in stage I (17%; P = 0.018). Complete concordance was observed between c-mos overexpression and elevated c-mos mRNA levels. Because c-mos gene amplification was not detected, its deregulated expression may be attributable to increased transcription. Of the c-mos positive [c-mos(P)] cases, 77% were associated with aneuploidy. Sequencing showed two silent mutations and one missense (R-->L) at codon 22, located in a region critical for c-mos stability. In contrast to the findings of some in vitro studies, c-mos(P) tumors had a lower mean AI score than the c-mos negative [c-mos(N)] tumors had, implying that induction of apoptosis may have been defective. Indeed, 86% of the tumors overexpressing c-mos showed p53 alterations. The carcinomas with concomitant alterations of c-mos and p53 [c-mos(P)/p53 positive] had significantly lower AI values (P < 0.001) and were more frequently associated with aneuploidy (P = 0.015) than the c-mos(N)/p53 negative tumors but not the c-mos(N)/p53 positive tumors, which suggests that p53 status is the main determinant of ploidy status and apoptosis in our series. This finding also strengthens the concept that wild-type p53 plays a "safeguard" role in preventing oncogene-mediated activation.
authors.affiliation: Department of Histology and Embryology, School of Medicine, University of Athens, Greece. histoclub@ath.forthnet.gr
authors.forename: V G
authors.initials: VG
authors.lastname: Gorgoulis
authors.forename: P
authors.initials: P
authors.lastname: Zacharatos
authors.forename: G
authors.initials: G
authors.lastname: Mariatos
authors.forename: T
authors.initials: T
authors.lastname: Liloglou
authors.forename: S
authors.initials: S
authors.lastname: Kokotas
authors.forename: N
authors.initials: N
authors.lastname: Kastrinakis
authors.forename: A
authors.initials: A
authors.lastname: Kotsinas
authors.forename: A
authors.initials: A
authors.lastname: Athanasiou
authors.forename: P
authors.initials: P
authors.lastname: Foukas
authors.forename: V
authors.initials: V
authors.lastname: Zoumpourlis
authors.forename: D
authors.initials: D
authors.lastname: Kletsas
authors.forename: J
authors.initials: J
authors.lastname: Ikonomopoulos
authors.forename: P J
authors.initials: PJ
authors.lastname: Asimacopoulos
authors.forename: C
authors.initials: C
authors.lastname: Kittas
authors.forename: J K
authors.initials: JK
authors.lastname: Field
mesh_terms: D000368:Aged; D000782:Aneuploidy; D017209:Apoptosis; D001483:Base Sequence; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002455:Cell Division; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000737:chemistry / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009154:Mutation; D009367:Neoplasm Staging; D010766:Phosphorylation; D018807:Polymorphism, Single-Stranded Conformational; D000090063:Proto-Oncogene Mas; D017231:Proto-Oncogene Proteins c-mos / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016019:Survival Analysis; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism
chemical_list: D004273:DNA, Neoplasm; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53; D017231:Proto-Oncogene Proteins c-mos; D020928:Mitogen-Activated Protein Kinases