The use of a recombinant lentiviral vector for ex vivo gene transfer into the rat CNS.
A major obstacle in ex vivo gene transfer has been the loss of transgene expression soon after implantation of the grafted transduced cells. Recently, a lentiviral vector system has been developed which has proven to express high levels of transgenes in vivo after direct injection into the tissue. In this study, we have investigated the use of such a vector for ex vivo gene transfer to the brain. A number of neural cell types were found to be permissive to transduction by the lentiviral vector in vitro and a majority of them expressed the transgene after transplantation to the rat brain. Transgene expression was detected up to 8 weeks post-grafting. These findings suggest that recombinant lentiviral vectors may be used for further development of ex vivo gene therapy protocols to the CNS.
Englund
U
U
Wallenberg Neuroscience Center, Division of Neurobiology, Lund University, Sweden.
Ericson
C
C
Rosenblad
C
C
Mandel
R J
RJ
Trono
D
D
Wictorin
K
K
Lundberg
C
C
D000818:Animals; D001921:Brain / Q000821:virology*; D016380:Brain Tissue Transplantation; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured / Q000166:cytology / Q000378:metabolism / Q000637:transplantation; D004274:DNA, Recombinant / Q000235:genetics; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000639:trends; D005822:Genetic Vectors / Q000235:genetics*; D049452:Green Fluorescent Proteins; D006801:Humans; D016086:Lentivirus / Q000235:genetics*; D008164:Luminescent Proteins / Q000235:genetics; D051379:Mice; D051381:Rats; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D014161:Transduction, Genetic; D019076:Transgenes / Q000235:genetics*
D004274:DNA, Recombinant; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins