A comparison of protocols for the optimisation of detection of bacteria using a surface acoustic wave (SAW) biosensor.
A dual channel surface acoustic wave (SAW) device has been used as a biosensor to detect two different microorganisms, Legionella and Escherichia coli, simultaneously. A series of experiments was conducted to optimise the use of the SAW for bacterial detection using a novel protocol of coating bacteria on the sensor surface prior to addition of the antibody. Results were compared with an experiment in which a conventional protocol was utilised, where antibody was coated on the sensor surface prior to exposure to bacteria. The concentration of bacteria that attached to the surface of the SAW device was related to the antibody that specifically bound to it and therefore to frequency in a dose dependent fashion. Unlike conventional microbiological techniques quantitative results can be obtained for Legionella and E. coli down to 10(6) cells per ml within 3 h. In addition E. coli was detected down to 10(5) cells per ml in a modified protocol using sheep IgG as a blocking agent.
Howe
E
E
Food Science Australia, North Ryde. effie.howe@foddscience.afisc.csiro.au
Harding
G
G
D001419:Bacteria / Q000302:isolation & purification*; D015374:Biosensing Techniques; D004926:Escherichia coli / Q000302:isolation & purification; D007118:Immunoassay; D007875:Legionella / Q000302:isolation & purification