Protein kinase D regulates the fission of cell surface destined transport carriers from the trans-Golgi network.
When a kinase inactive form of Protein Kinase D (PKD-K618N) was expressed in HeLa cells, it localized to the trans-Golgi network (TGN) and caused extensive tubulation. Cargo that was destined for the plasma membrane was found in PKD-K618N-containing tubes but the tubes did not detach from the TGN. As a result, the transfer of cargo from TGN to the plasma membrane was inhibited. We have also demonstrated the formation and subsequent detachment of cargo-containing tubes from the TGN in cells stably expressing low levels of PKD-K618N. Our results suggest that PKD regulates the fission from the TGN of transport carriers that are en route to the cell surface.
Liljedahl
M
M
Biology Department, University of California, San Diego, La Jolla, CA 92093, USA.
Maeda
Y
Y
Colanzi
A
A
Ayala
I
I
Van Lint
J
J
Malhotra
V
V
D001692:Biological Transport; D015704:CD4 Antigens / Q000378:metabolism; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology* / Q000378:metabolism; D002966:Clathrin / Q000378:metabolism; D020756:Coatomer Protein / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004721:Endoplasmic Reticulum / Q000378:metabolism; D011992:Endosomes / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005982:Glutathione Transferase / Q000378:metabolism; D006023:Glycoproteins; D006367:HeLa Cells; D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism; D008565:Membrane Proteins; D010766:Phosphorylation; D011233:Precipitin Tests; D011493:Protein Kinase C / Q000235:genetics / Q000502:physiology*; D021381:Protein Transport; D011994:Recombinant Proteins / Q000378:metabolism; D013696:Temperature; D013997:Time Factors; D014162:Transfection; D014759:Viral Envelope Proteins / Q000378:metabolism; D021601:trans-Golgi Network / Q000378:metabolism*
D015704:CD4 Antigens; D002966:Clathrin; D020756:Coatomer Protein; C028391:G protein, vesicular stomatitis virus; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D011994:Recombinant Proteins; C072237:TGOLN2 protein, human; D014759:Viral Envelope Proteins; D005982:Glutathione Transferase; C089212:protein kinase D; D011493:Protein Kinase C