Enhanced production of vascular endothelial growth factor by human monocytic cells stimulated with endotoxin through transcription factor SP-1.
The effect of endotoxin on the regulation of vascular endothelial growth factor (VEGF) mRNA expression in human monocytic (THP-1) cells was examined. Endotoxic lipopolysaccharide (LPS) from Escherichia coli and synthetic E. coli-type lipid A (LA-15-PP) enhanced VEGF mRNA expression. LPS-induced VEGF mRNA accumulation was regulated, at least in part, at the transcriptional level. Enhancement of VEGF gene expression by LPS was shown by gel shift analysis and use of transcription factor inhibitors to be mediated via the activation of SP-1.
Tetsuya
T
Sakuta
Kenji
K
Matsushita
Department of Operative Dentistry and Endodontology, Kagoshima University Dental School, Kagoshima 890-8544, *Department of Preventive Dentistry, Kyushu University Faculty of Dentistry, Fukuoka 812-8582, †Department of Clinical Laboratory Medicine, Kagoshima University Medical School, Kagoshima 890-8520 and ‡Department of Microbiology and Immunology, Tohoku University School of Dentistry, Sendai 980-8575, Japan.
Noboru
N
Yamaguchi
Tohru
T
Oyama
Rie
R
Motani
Department of Operative Dentistry and Endodontology, Kagoshima University Dental School, Kagoshima 890-8544, *Department of Preventive Dentistry, Kyushu University Faculty of Dentistry, Fukuoka 812-8582, †Department of Clinical Laboratory Medicine, Kagoshima University Medical School, Kagoshima 890-8520 and ‡Department of Microbiology and Immunology, Tohoku University School of Dentistry, Sendai 980-8575, Japan.
Toshihiko
T
Koga
Shigetaka
S
Nagaoka
Department of Operative Dentistry and Endodontology, Kagoshima University Dental School, Kagoshima 890-8544, *Department of Preventive Dentistry, Kyushu University Faculty of Dentistry, Fukuoka 812-8582, †Department of Clinical Laboratory Medicine, Kagoshima University Medical School, Kagoshima 890-8520 and ‡Department of Microbiology and Immunology, Tohoku University School of Dentistry, Sendai 980-8575, Japan.
Kazuhiro
K
Abeyama
Department of Operative Dentistry and Endodontology, Kagoshima University Dental School, Kagoshima 890-8544, *Department of Preventive Dentistry, Kyushu University Faculty of Dentistry, Fukuoka 812-8582, †Department of Clinical Laboratory Medicine, Kagoshima University Medical School, Kagoshima 890-8520 and ‡Department of Microbiology and Immunology, Tohoku University School of Dentistry, Sendai 980-8575, Japan.
Ikuro
I
Maruyama
Department of Operative Dentistry and Endodontology, Kagoshima University Dental School, Kagoshima 890-8544, *Department of Preventive Dentistry, Kyushu University Faculty of Dentistry, Fukuoka 812-8582, †Department of Clinical Laboratory Medicine, Kagoshima University Medical School, Kagoshima 890-8520 and ‡Department of Microbiology and Immunology, Tohoku University School of Dentistry, Sendai 980-8575, Japan.
Haruhiko
H
Takada
Mitsuo
M
Torii
D002478:Cells, Cultured; D016228:Endothelial Growth Factors / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D008050:Lipid A / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008222:Lymphokines / Q000096:biosynthesis* / Q000235:genetics; D009000:Monocytes / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D016329:Sp1 Transcription Factor / Q000502:physiology*; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D016228:Endothelial Growth Factors; D008050:Lipid A; D008070:Lipopolysaccharides; D008222:Lymphokines; D012333:RNA, Messenger; D016329:Sp1 Transcription Factor; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors