Stereotactic core biopsy breast and blood cell by-products: a source of material for molecular genetics research--initial experience.
Stereotactic core biopsy washings and blood drop samples, routinely discarded by-products, provide satisfactory fresh cellular material for flow cytometry and molecular genetics microsatellite polymerase chain reaction (PCR) analysis for detection of loss of DNA alleles (loss of heterozygosity). Cytokeratin-positive (epithelial) cells from the core biopsy washings were sorted by means of flow cytometry prior to PCR analysis. DNA allele loss was detected in benign breast epithelial cells in three (20%) of 15 patients.
Departments of Diagnostic Imaging, Roswell Park Cancer Institute, School of Medicine and Biomedical Sciences, SUNY, Elm and Carlton Sts, Buffalo, NY 14263, USA. Paul.Stomper@roswellpark.org
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Stomper
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Stewart
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Stoler
D000328:Adult; D000368:Aged; D001706:Biopsy; D001773:Blood Cells; D001940:Breast / Q000473:pathology*; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007633:Keratins / Q000032:analysis; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D013238:Stereotaxic Techniques
D007633:Keratins