Identification of Lhcb1/Lhcb2/Lhcb3 heterotrimers of the main light-harvesting chlorophyll a/b-protein complex of Photosystem II (LHC II).
Using non-denaturing isoelectric focusing in polyacrylamide vertical slab gel, we have purified to homogeneity three trimeric subcomplexes of LHC II from Arabidopsis thylakoid membranes. The polypeptide composition of the subcomplexes were studied by immunoblotting. Our results indicate the existence in vivo of LHC II heterotrimers containing Lhcb1, Lhcb2 and Lhcb3 gene products.
Jackowski
G
G
Department of Plant Physiology, Adam Mickiewicz University, Poznañ, Poland. grzesiek@main.amu.edu.pl
Kacprzak
K
K
Jansson
S
S
D001059:Apoproteins / Q000737:chemistry; D017360:Arabidopsis / Q000737:chemistry*; D029681:Arabidopsis Proteins / Q000737:chemistry*; D002942:Circular Dichroism; D007525:Isoelectric Focusing; D045342:Light-Harvesting Protein Complexes / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry*; D045332:Photosystem II Protein Complex; D020524:Thylakoids / Q000737:chemistry
D001059:Apoproteins; D029681:Arabidopsis Proteins; C000602509:Lhcb2 protein, Arabidopsis; D045342:Light-Harvesting Protein Complexes; D010455:Peptides; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex