A liquid chromatography-tandem mass spectrometry method for fluazifop residue analysis in crops.
An LC-MS-MS assay is described for fluazifop residue analysis in crops. The residues are extracted with acidified organic solvent, the esters and conjugates are hydrolysed with 6 M hydrochloric acid, then the extracts are cleaned-up by solid phase extraction using C2(EC) and Si cartridges in tandem. Quantitative analysis is performed by gradient liquid chromatography coupled to triple quadrupole mass spectrometer using atmospheric pressure chemical ionisation. All fluazifop-P-butyl, free fluazifop-P and any conjugates are quantified as fluazifop-P. The limit of quantification is 0.01-0.05 mg/kg depending on crop matrices. The clean-up method is also suitable for LC-UV analysis with a compromise in higher limit of quantification 0.05-0.2 mg/kg.
Zeneca Agrochemicals, Jealott's Hill International Research Centre, Bracknell, UK. elek.e.bolygo@aguk.zeneca.com
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Bolygo
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Boseley
D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004095:Dihydropyridines / Q000032:analysis*; D006540:Herbicides / Q000032:analysis*; D006868:Hydrolysis; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D010573:Pesticide Residues / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D004095:Dihydropyridines; D006540:Herbicides; D007202:Indicators and Reagents; D010573:Pesticide Residues; C050582:fluazifop