Toxic shock syndrome toxin-1 accelerated collagen-induced arthritis in mice.
The aim of this study was to explore the roles of toxic shock syndrome toxin-1 (TSST-1) in collagen-induced arthritis (CIA). DBA/1 mice were immunized with type II collagen (CII) and treated with TSST-1. Intraperitoneal and intravenous injections of TSST-1 aggravated CIA, enhancing its incidence and severity. CIA was accompanied by an increase in anti-CII IgG Ab levels. Intraperitoneal administration with TSST-1 enhanced IFN-gamma, TNF-alpha, and IL-4 production in DBA/1 mice. We discovered the mRNA expressions of IFN-gamma, IL-2, TNF-alpha, IL-1beta, and iNOS in spleen cells stimulated with TSST-1 in vitro. However, IL-12 and IL-4 mRNA expression were seen constitutively without stimulation. Only a little increase of IL-12 and IL-4 mRNA expression was seen at 2-3 h after treatment with TSST-1. Our experiments demonstrated that CIA was aggravated by the treatment with TSST-1, which may have induced various proinflammatory cytokines and the production of both Th1 and Th2 cytokines.
Department of Orthopedic Surgery, Hamamatsu University School of Medicine, Hamamatsu, Japan.
Y
Y
Kageyama
Y
Y
Koide
T
T
Nagata
M
M
Uchijima
A
A
Yoshida
T
T
Arai
T
T
Miura
C
C
Miyamoto
A
A
Nagano
D000818:Animals; D000917:Antibody Formation; D001172:Arthritis, Rheumatoid / Q000453:epidemiology / Q000276:immunology / Q000503:physiopathology*; D001427:Bacterial Toxins; D003094:Collagen / Q000276:immunology*; D016207:Cytokines / Q000235:genetics; D004195:Disease Models, Animal; D004768:Enterotoxins / Q000008:administration & dosage / Q000276:immunology*; D005260:Female; D015870:Gene Expression; D007074:Immunoglobulin G / Q000096:biosynthesis; D015994:Incidence; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics; D015847:Interleukin-4 / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008811:Mice, Inbred DBA; D019001:Nitric Oxide Synthase / Q000235:genetics; D052247:Nitric Oxide Synthase Type II; D012333:RNA, Messenger; D018089:Superantigens; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics
D001427:Bacterial Toxins; D016207:Cytokines; D004768:Enterotoxins; D007074:Immunoglobulin G; D012333:RNA, Messenger; D018089:Superantigens; D014409:Tumor Necrosis Factor-alpha; C030107:enterotoxin F, Staphylococcal; D015847:Interleukin-4; D007371:Interferon-gamma; D003094:Collagen; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse