[Use of atomic force microscopy to study the morphology and structure of viruses].
The paper describes AFM methodic approaches in the investigation of viruses (TMV, phage T4). A possibility is shown to use this method to obtain high-quality three-dimensional image of objects without additional treatment with salts of heavy metals. The phage T4 was also a model to study the object density, using the phase shift of the AFM. Such researches can determine the internal structure of a virion and folding of nucleic acid inside a virion.
Polishchuk
V P
VP
Taras Shevchenko Kyiv National University, 64 Vladimirskaya St., Kyiv, 01033, Ukraine.
Tyvonchuk
T P
TP
Rengevich
Ie V
IeV
Beketov
G V
GV
Budzanivskaia
I G
IG
Boĭko
A L
AL
D017122:Bacteriophage T4 / Q000648:ultrastructure*; D021621:Imaging, Three-Dimensional; D018625:Microscopy, Atomic Force / Q000379:methods*; D014027:Tobacco Mosaic Virus / Q000648:ultrastructure*