Expression study of genes involved in iron metabolism in human tissues.
Iron is required in all organisms for crucial functions, as a number of proteins need iron for activity. Mutations of the genes encoding proteins involved in iron uptake, transport, and utilization result in various human disorders or animal models with very different clinical presentations and organ involvement. However, little is known concerning the expression of iron metabolism genes in various human tissues and their eventual concerted regulation. We therefore examined the expression levels of various genes involved in iron uptake, reduction, and storage, in Fe-S protein biogenesis, in mitochondrial electron transport chain, plus the two SOD genes, in human adult tissues by Northern blot analysis. We observed that most of these genes were ubiquitously expressed, but that their transcript showed strongly different levels in the various tissues investigated denoting different mechanisms for iron utilization in various organs. However, surprisingly, no correlation could be made between expression pattern of these genes and the clinical presentation resulting in their mutations.
Rötig
A
A
INSERM U393, Hôpital Necker-Enfants Malades, 149 rue de Sèvres, Paris, 75015, France. roetig@necker.fr
Chantrel-Groussard
K
K
Munnich
A
A
Rustin
P
P
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D020869:Gene Expression Profiling; D006801:Humans; D007501:Iron / Q000378:metabolism*; D051379:Mice; D008928:Mitochondria / Q000378:metabolism; D011506:Proteins / Q000235:genetics*
D000975:Antioxidants; D011506:Proteins; D007501:Iron