Inhibition of concanavalin A-induced mice hepatitis by coumarin derivatives.
The effects of coumarin derivatives, osthole, imperatorin, Pd-Ia, Pd-II and Pd-III, on mice concanavalin A (Con A) (0.2 mg/mouse, i.v.)-induced hepatitis were studied. At the dose of 200 mg/kg (i.p.), these coumarins inhibited more than 90% of the Con A-induced elevation of plasma alanine aminotransferase activity, but glycyrrhizin (200 mg/kg, i.p.) caused only 45% inhibition. At the dose of 100 mg/kg (i.p.), osthole produced the strongest inhibition among these coumarins. The inhibitory activity of osthole is lost when its 7-methoxy group is replaced by a 7-hydroxy group to form osthenol. The present results showed that coumarin derivatives inhibited Con A-induced hepatitis, with osthole being the most inhibitory.
Okamoto
T
T
Research Laboratories, Nippon Chemiphar Co., Ltd., Misato, Saitama, Japan. ncokamot@green.ocn.ne.jp
Yoshida
S
S
Kobayashi
T
T
Okabe
S
S
D000410:Alanine Transaminase / Q000097:blood / Q000187:drug effects; D000818:Animals; D000925:Anticoagulants / Q000737:chemistry / Q000494:pharmacology*; D003208:Concanavalin A / Q000037:antagonists & inhibitors*; D003374:Coumarins / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D011564:Furocoumarins / Q000737:chemistry / Q000494:pharmacology; D019695:Glycyrrhizic Acid / Q000494:pharmacology; D006520:Hepatitis, Animal / Q000139:chemically induced* / Q000517:prevention & control; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C
D000925:Anticoagulants; D003374:Coumarins; D011564:Furocoumarins; D003208:Concanavalin A; D019695:Glycyrrhizic Acid; C089518:3'-angeloyloxy-4'-acetoxy-3',4'-dihydroseselin; D000410:Alanine Transaminase; C031534:imperatorin; C046627:osthol