Direct and indirect inactivation of Microcystis aeruginosa by UV-radiation.
Excessive algal growth in drinking water sources like lakes and reservoirs is responsible for filter-clogging, undesirable taste and odor, disinfection-by-product formation and toxin generation. Although various methods are currently being used to control algal bloom, their successes are limited. Some water utilities routinely use copper sulfate to control excessive algal growth. But there is a growing concern against its use mainly because it is non-specific to target algae and kills many non-target species. In this study, the scope of using UV-radiation to control algal growth was assessed using Microcystis aeruginosa as test species. A UV-dose of 75 mW s cm(-2) was found to be lethal to M. aeruginosa. A smaller dose of 37 mW s cm(-2) prevented growth for about 7 days. It was found that UV-radiation may increase the specific gravity of the cells and thus may adversely affect the ability of the cells to remain in suspension. Three days after a UV-dose of 75 mW s cm(-2), almost all the cells settled to the bottom of the incubation tubes, whereas all the unirradiated cells remained in suspension. It was also observed that UV-radiation on algal extracellular products has a significant residual effect and can contribute to algal growth control. The extent of residual effect depends on the UV-dose and can continue even for 7 days. UV-radiation was found to produce H2O2 in the microM level concentration. But at such level, H2O2 itself is not likely to cause the residual effect that was found in this study.
Alam
Z B
ZB
Department of Urban Engineering, The University of Tokyo, Bunkyo, Japan. alam@env.t.u-tokyo.ac.jp
Otaki
M
M
Furumai
H
H
Ohgaki
S
S
D005618:Fresh Water / Q000032:analysis / Q000382:microbiology; D006861:Hydrogen Peroxide / Q000032:analysis; D046931:Microcystis / Q000254:growth & development / Q000528:radiation effects*; D014466:Ultraviolet Rays; D014871:Water Microbiology; D018508:Water Purification / Q000379:methods; D014881:Water Supply / Q000032:analysis
D006861:Hydrogen Peroxide