Distal airspace enlargement in the fawn-hooded rat: influences of aging and alveolar wall destruction.
BACKGROUND
A recent study has revealed that the peripheral airspace in the lungs of the fawn-hooded rat (FHR) is enlarged. However, morphological and functional factors of the FHR lung have not been fully investigated.


OBJECTIVE
The purpose of our study was to examine the structural and functional changes in the FHR lung and to investigate the influence of aging on this process. Furthermore, morphological and functional measurements of the lungs of FHRs (4-48 weeks of age) were performed and the results compared with those of age-matched Wistar control rats (WCRs).


METHODS
All animals were studied under controlled conditions, and morphological and functional changes of the lungs were examined. Measurements of body and lung weights were recorded, and the lungs were subjected to morphological evaluation. Morphological measurements: mean linear intercept (MLI) and destructive index were determined. Functional evaluation of the lungs: total lung capacity, pressure-volume curve, and exponential constant (K) which describes the shape of the curve were analyzed. In addition, right ventricular hypertrophy measurements were performed to assess the severity of pulmonary hypertension. Statistical analysis was performed using the unpaired t test, analysis of variance, and the Fisher post hoc test (p < 0.05).


RESULTS
Morphological analysis revealed a significant increase in airspace size (MLI) in all FHRs as compared with the WCRs which was evident from an early age (4 weeks). The increase in MLI did not progress age dependently in the FHR, whereas a tendency for an age-dependent increase in MLI was observed in the WCR. The destructive index measurements revealed that the increase in MLI of FHR was not accompanied by alveolar wall destruction. Concerning the functional examination, a leftward and upward shift of the pressure-volume curve was observed in the FHRs as compared with the WCRs at all ages. As compared with the WCRs, a higher K value was observed in all FHRs which was evident from an early age (4 weeks). Age-dependent changes similar to those in MLI were observed in the K in both rat strains.


CONCLUSIONS
The results of our study suggest that FHRs manifest characteristics of distal airspace enlargement accompanied by increased lung distensibility without alveolar wall destruction at an early age and that the changes do not progress age dependently. Neither an accelerated aging process nor destruction of the alveolar walls appears to be the mechanism responsible for the enlarged airspace in this rat strain.
Morio
Y
Y
Department of Respiratory Medicine, Juntendo University School of Medicine, Tokyo, Japan.
Muramatsu
M
M
Takahashi
K
K
Teramoto
S
S
Oka
T
T
Fukuchi
Y
Y
D000375:Aging / Q000473:pathology*; D000403:Airway Resistance; D000818:Animals; D000831:Animals, Newborn; D046508:Culture Techniques; D005260:Female; D006976:Hypertension, Pulmonary / Q000473:pathology; D017380:Hypertrophy, Right Ventricular; D008168:Lung / Q000254:growth & development* / Q000473:pathology*; D008297:Male; D023421:Models, Animal; D009929:Organ Size; D011650:Pulmonary Alveoli / Q000473:pathology*; D051381:Rats; D011919:Rats, Inbred Strains; D017208:Rats, Wistar; D012016:Reference Values