[A new thermostable proteinase from Thermoactinomyces SP.27a. Cloning and gene expression].
A library of Thermoactinomyces sp. 27a, producer of thermostable proteases of different groups, has been created. Gene coding for thermostable neutral proteinase was cloned and expressed in Bac. subtilis cells. Restriction map for cloned DNA fragment was created and physicochemical parameters of recombinant proteinase were characterized. The thermostability and optimum of proteolytic activity of the enzyme was lower than in the natural Thermoactinomyces sp. strain, which can be due to heterologous expression of the gene coding for thermostable protein in the mesophilic host.
Zabolotskaia
M V
MV
Institute of Molecular Genetics, Russian Academy of Sciences, Moscow.
Nosovskaia
E A
EA
Kaplun
M A
MA
Tsaplina
I A
IA
Akimkina
T V
TV
D003001:Cloning, Molecular; D010450:Endopeptidases / Q000235:genetics* / Q000378:metabolism; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008848:Micromonosporaceae / Q000201:enzymology*; D015183:Restriction Mapping
D010450:Endopeptidases