Enhanced green fluorescent protein as a useful tag for rapid identification of homozygous transgenic mice.
We developed a technique that simplifies the process of confirming homozygous transgenics at preimplantation stages, which are the earliest stages used in test breeding, using enhanced green fluorescent protein as a tag. All the blastocysts obtained by mating with the combination of Tg/Tg male (homozygous for transgene) x +/+ female exhibited fluorescence.
Molecular Medicine Research Center, The Institute of Medical Sciences, Tokai University, Bohseidai, Isehara, 259-1193, Kanagawa, Japan. masasato@is.icc.u-tokai.ac.jp
M
M
Sato
T
T
Watanabe
A
A
Oshida
A
A
Nagashima
J
J
Miyazaki
M
M
Kimura
D000818:Animals; D001755:Blastocyst / Q000378:metabolism; D005260:Female; D005456:Fluorescent Dyes / Q000378:metabolism*; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins; D006720:Homozygote; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D019076:Transgenes / Q000235:genetics
D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins