Interfacing matrix-assisted laser desorption/ionization mass spectrometry with column and planar separations.
This paper presents a critical review of off-line and on-line coupling of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry to liquid column separations (e.g. HPLC, GPC and CE) and planar separations (e.g. PAGE and TLC). Off-line MALDI analysis of fractions collected from HPLC, GPC and CE or spots scraped and extracted from TLC and PAGE has already become a routine practice for many laboratories. MALDI has also been used to obtain mass spectra directly from TLC plates and PAGE. The direct analysis methods range from dot-blotted samples to two-dimensional scanning of the entire gels/plates. Various combinations of on-line coupling of MALDI with column separations are also reviewed. The review discusses the strengths and limitations associated with different off-line and on-line coupling approaches.
Rohm and Haas Company, Research Laboratories, Spring House, PA 19477-0904, USA. arkady.gusev@rohmhaas.com
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D000818:Animals; D002850:Chromatography, Gel / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D002855:Chromatography, Thin Layer / Q000295:instrumentation / Q000379:methods; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods