Immunopotentiation of Staphylococcus aureus type 5 capsular polysaccharide co-entrapped in liposomes with alpha-toxin.
Immunopotentiation of Staphylococcus aureus type 5 capsular polysaccharide (CP5) by use of liposomes as an alternative to protein-polysaccharide conjugates was investigated. Mice were immunized twice with cationic liposomes containing CP5 alone or CP5 co-entrapped with alpha-toxin or heat-detoxified alpha-toxin. Immunogenicity of these different antigens was compared with CP5-alpha-toxin conjugates. Antibodies against CP5 were elicited in mice immunized with conjugates or liposomes containing co-entrapped CP5 and alpha-toxin. Liposomes containing CP5 alone or co-entrapped CP5 and alpha-toxoid failed to induce antibodies against CP5. All the preparations entailed an antibody response against alpha-toxin and highest antibody and neutralizing activity titers were obtained with liposomes. These results show that liposomes can be used to immunopotentiate CP5, however, a co-incorporated protein able to insert lipids bilayers is probably required.
Laboratoire de Pathologie Infectieuse et Immunologie, Institut National de la Recherche Agronomique, 37380, Nouzilly, France.
C
C
Burgeot
F B
FB
Gilbert
B
B
Poutrel
D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000097:blood; D016667:Bacterial Capsules / Q000008:administration & dosage* / Q000276:immunology*; D001427:Bacterial Toxins / Q000008:administration & dosage*; D001428:Bacterial Vaccines / Q000008:administration & dosage; D005260:Female; D006460:Hemolysin Proteins / Q000008:administration & dosage*; D006461:Hemolysis; D007114:Immunization; D008081:Liposomes; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D013211:Staphylococcus aureus / Q000276:immunology*; D018074:Vaccines, Conjugate / Q000008:administration & dosage
D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D006460:Hemolysin Proteins; D008081:Liposomes; D018074:Vaccines, Conjugate; C054106:capsular polysaccharide, type 5, Staphylococcus aureus; C021091:staphylococcal alpha-toxin