[Protein tyrosine kinase (PTK) activities during the induction of apoptosis by arsenic trioxide (As2O3)].
OBJECTIVE
To explore the mechanism of arsenic trioxide (As2O3) induced apoptosis in K562 cell line.


METHODS
Immunoprecipitation was used to obtain BCR/ABL and ABL proteins. The activities of PTK and tyrosine phosphorylation were measured by biochemical method and Western blot, respectively.


RESULTS
As2O3 reduced the PTK activities of cytosol and membrane proteins and BCR/ABL and ABL proteins. The tyrosine phosphorylation of 180,000 and 125,000 proteins declined.


CONCLUSIONS
As2O3 may interfere the signal transduction of BCR/ABL protein by reducing its PTK activity and induce K562 cell apoptosis.
Shanghai Institute of Hematology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025.
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Xiao
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Sun
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Wu
D017209:Apoptosis; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology*; D016044:Fusion Proteins, bcr-abl / Q000378:metabolism*; D006801:Humans; D020014:K562 Cells / Q000201:enzymology / Q000473:pathology; D016374:Oncogene Proteins v-abl / Q000378:metabolism; D010087:Oxides / Q000494:pharmacology*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*
D001152:Arsenicals; D016374:Oncogene Proteins v-abl; D010087:Oxides; D011505:Protein-Tyrosine Kinases; D016044:Fusion Proteins, bcr-abl; D000077237:Arsenic Trioxide