Synthesis of of beta-amyloid precursor peptide and presenilin segments.
It seems likely that the beta-amyloid precursor protein (APP) and the presenilins (PS-1/2) play important roles in the development of Alzheimer's disease (AD). Attempts to mimic the biochemical actions of these proteins are often made by the application of fragments of these proteins. However, the synthesis of these segments by conventional methods of peptide synthesis is problematic. We have synthesized several C-terminal fragments of APP and PS-1/2 by solid-phase synthesis through combination of automatic and manual methods of synthesis. This permits solution of the 'difficult sequences' in the solid-phase synthesis of these peptides. Some details of the syntheses of nine segments are presented in this paper.
Baláspiri
L
L
Department of Neurochemistry and Neurotoxicology, Stockholm University, Sweden. balaspiri@mdche.szote.u-szeged.hu
Langel
U
U
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000138:chemical synthesis; D016564:Amyloid beta-Protein Precursor / Q000138:chemical synthesis* / Q000737:chemistry; D005449:Fluorenes / Q000138:chemical synthesis; D005563:Formic Acid Esters / Q000138:chemical synthesis; D006801:Humans; D008565:Membrane Proteins / Q000138:chemical synthesis* / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D053764:Presenilin-1; D053766:Presenilin-2; D012117:Resins, Synthetic / Q000138:chemical synthesis; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000596:Amino Acids; D016564:Amyloid beta-Protein Precursor; D005449:Fluorenes; D005563:Formic Acid Esters; D008565:Membrane Proteins; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids; C508853:PSEN1 protein, human; C508861:PSEN2 protein, human; D010455:Peptides; D053764:Presenilin-1; D053766:Presenilin-2; D012117:Resins, Synthetic; C075825:Rink amide resin; C000345:t-butyloxycarbonyl group