Crystallization and preliminary X-ray crystallographic analysis of RNase HIII from Bacillus subtilis.
The genome of Bacillus subtilis contains three different genes encoding RNase H homologs: RNases HI, HII and HIII. RNase HIII from B. subtilis degrades RNA in RNA-DNA hybrids in an Mg(2+)-dependent manner like Escherichia coli RNase HI. However, they belong to different classes; the former belongs to the 'class II' or 'large' RNase H family, while the latter belongs to the 'class I' or 'small' RNase H family. RNase HIII of B. subtilis has been overexpressed in E. coli and crystallized at 296 K using sodium formate as a precipitant. The native X-ray diffraction data have been collected to 2.8 A resolution using synchrotron radiation. The crystals are hexagonal, belonging to the space group P6(1), with unit-cell parameters a = b = 86.89, c = 214.49 A, alpha = beta = 90.0, gamma = 120.0 degrees. A self-rotation function calculation indicated the presence of two monomers of the recombinant RNase HIII in the crystallographic asymmetric unit, giving a V(M) of 3.43 A(3) Da(-1) and a solvent content of 64.2%.
School of Chemistry and Molecular Engineering, Seoul National University, Seoul 151-742, South Korea.
J E
JE
Kwak
J Y
JY
Lee
B W
BW
Han
J H
JH
Moon
S H
SH
Sohn
I S
IS
Park
B G
BG
Kim
S W
SW
Suh
D001412:Bacillus subtilis / Q000737:chemistry*; D001426:Bacterial Proteins; D003460:Crystallization; D018360:Crystallography, X-Ray; D011487:Protein Conformation; D016914:Ribonuclease H / Q000737:chemistry*; D012260:Ribonucleases
D001426:Bacterial Proteins; D012260:Ribonucleases; C117079:ribonuclease HIII; D016914:Ribonuclease H