[Cloning and function of sanJ--a gene involved in nikkomycins biosynthesis of Streptomyces ansochromogenes].
A 7.5 kb DNA fragment was identified from the cosmid library of Streptomyces ansochromogenesus using a partial DNA fragment involved in the nikkomycin biosynthesis as a probe. It was cloned into pBluescript M13- and the resulting recombinant plasmid was designated as pNL2200. The nucleotide sequence of 2.3 kb Sal I -BamH I DNA fragment was determined and analysed. The result indicated that the fragment contains one complete open reading frame (ORF); The start codon is GTG at 271 position and the stop codon is TGA at 1,954 position. It contains 1,686 base pairs and encodes a protein with 561 amino acids. The deduced product has 44% identity with the ligase belong to the superfamily of adenylate-forming enzyme. The experiment of gene disruption showed that the sanJ gene was closely related to nikkomycins biosynthesis of Streptomyces ansochromogenes. It was designated as sanJ.
Nie
L P
LP
Institute of Microbiology, The Chinese Academy of Sciences, Beijing 100080, China.
Tian
Y Q
YQ
Tan
H R
HR
D000595:Amino Acid Sequence; D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D000935:Antifungal Agents / Q000096:biosynthesis*; D001483:Base Sequence; D003001:Cloning, Molecular; D003066:Coenzyme A Ligases; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D013302:Streptomyces / Q000235:genetics*
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; C011952:nikkomycin; D003066:Coenzyme A Ligases; C512544:SanJ protein, Streptomyces ansochromogenes