Different susceptibility to oxidation of proline and arginine residues of apolipoprotein B-100 among subspecies of low density lipoproteins.
gamma-Glutamyl semialdehyde is a primary oxidation product of apolipoprotein (apo) B-100 proline (Pro) and arginine (Arg) side chain residues. By reduction gamma-glutamyl semialdehyde forms 5-hydroxy-2-aminovaleric acid (HAVA). Here we describe the application of sensitive and specific HAVA measurement to characterize the formation of gamma-glutamyl semialdehyde in several domains of apoB-100 in LDL(1) (S(f) 7-12) and LDL(2) (S(f) 0-7) subfractions subjected to oxidative damage in the presence of iron in vitro. Results suggest that susceptibility of apoB-100 Pro and Arg residues toward oxygen radicals drastically changes along the lipoprotein metabolic cascade.
Pietzsch
J
J
Institute and Policlinic of Clinical Metabolic Research, Medical Faculty Carl Gustav Carus, Technical University, Fetscherstrasse 74, D-01307 Dresden, Germany. jens.pietzsch@mailbox.tu-dresden.de
Julius
U
U
D000328:Adult; D000596:Amino Acids / Q000737:chemistry; D053299:Apolipoprotein B-100; D001055:Apolipoproteins B / Q000097:blood / Q000737:chemistry*; D001120:Arginine / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D005971:Glutamates / Q000737:chemistry; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood / Q000737:chemistry*; D008297:Male; D010084:Oxidation-Reduction; D011392:Proline / Q000737:chemistry*; D017382:Reactive Oxygen Species
D000596:Amino Acids; D053299:Apolipoprotein B-100; D001055:Apolipoproteins B; D005971:Glutamates; D008077:Lipoproteins, LDL; D017382:Reactive Oxygen Species; C021199:glutamic acid gamma-semialdehyde; C004387:2-amino-5-hydroxyvaleric acid; D001120:Arginine; D011392:Proline