[Cloning and function of sanJ--a gene involved in nikkomycins biosynthesis of Streptomyces ansochromogenes].
A 7.5 kb DNA fragment was identified from the cosmid library of Streptomyces ansochromogenesus using a partial DNA fragment involved in the nikkomycin biosynthesis as a probe. It was cloned into pBluescript M13- and the resulting recombinant plasmid was designated as pNL2200. The nucleotide sequence of 2.3 kb Sal I -BamH I DNA fragment was determined and analysed. The result indicated that the fragment contains one complete open reading frame (ORF); The start codon is GTG at 271 position and the stop codon is TGA at 1,954 position. It contains 1,686 base pairs and encodes a protein with 561 amino acids. The deduced product has 44% identity with the ligase belong to the superfamily of adenylate-forming enzyme. The experiment of gene disruption showed that the sanJ gene was closely related to nikkomycins biosynthesis of Streptomyces ansochromogenes. It was designated as sanJ.
Institute of Microbiology, The Chinese Academy of Sciences, Beijing 100080, China.
L P
LP
Nie
Y Q
YQ
Tian
H R
HR
Tan
D000595:Amino Acid Sequence; D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D000935:Antifungal Agents / Q000096:biosynthesis*; D001483:Base Sequence; D003001:Cloning, Molecular; D003066:Coenzyme A Ligases; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D013302:Streptomyces / Q000235:genetics*
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; C011952:nikkomycin; D003066:Coenzyme A Ligases; C512544:SanJ protein, Streptomyces ansochromogenes