[Detection and significance of HPV16, 18 infection, P53 overexpression and telomerase activity in patients with lung cancer].
OBJECTIVE
To investigate if HPV16, 18 infection, P53 expression and telomerase activity have the differences among different ethnic patient groups with lung cancer, and their significance.


METHODS
HPV16, 18-DNA, P53 protein overexpression in 110 specimens of lung cancer were detected by PCR and immunohistochemistry, respectively. And telomerase activity in 22 specimens of fresh lung cancer tissues was detected by PCR-TRAP.


RESULTS
The positive rate of HPV16, 18-DNA in the lung cancer group was higher than the normal tissues group and inflammatory lesions group (all P < 0.01). The positive rate of P53 protein overexpression was 57% in the lung cancer group, negative in the normal tissues group (P < 0.01). The differences of HPV16, 18 infection and P53 overexpression have no statistic significance among different ethnic groups (all P > 0.05). The telomerase activity was higher in the cancer tissues (86%) compared with the adjacent normal tissues (22%, P < 0.01).


CONCLUSIONS
HPV16, 18 infection might have etiologic significance for lung cancer and make p53 gene overexpression to cause carcinogenesis, but each might have its own carcinogenic process. The high telomerase activity could be a marker of biomolecular change in lung cancer. The different ethnic background of HPV16, 18 infection and P53 overexpression of lung cancer need to do further study with a large number of specimens in the future.
Department of Radio-therapy, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830053, China.
H
H
Niyaz
C
C
Zhao
Y
Y
Li
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000821:virology; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism* / Q000821:virology; D008297:Male; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D019098:Telomerase / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis*; D014412:Tumor Virus Infections / Q000378:metabolism*
D016159:Tumor Suppressor Protein p53; D019098:Telomerase