Inhibition by retinoids of antigen-induced IL-4 production in rat mast cell line RBL-2H3.
The retinoic acid receptor (RAR) agonists, Re80 and Am80, partially inhibited the antigen-induced IL-4 production by rat mast cell line RBL-2H3 in a concentration-dependent manner (0.1 to 1000 nM). Both Re80 and Am80 also reduced the antigen-induced increase in IL-4 mRNA levels. The RAR antagonist LE540 at 4 microM reversed Re80 (100 nM)- and Am80 (100 nM)-induced inhibition of IL-4 production. The retinoid X receptor agonist HX600 (1 microM) by itself did not affect IL-4 production, but enhanced the inhibitory effect of Re80 (10 nM) and of Am80 (10 nM). Cyclosporin A suppressed the antigen-induced IL-4 production almost completely at 0.3 microM. These findings indicated that the antigen-induced IL-4 production by RBL-2H3 cells is partially inhibited by retinoids via RAR-dependent mechanisms.
Laboratory of Pathophysiological Biochemistry, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan. hirasawa@mail.pharm.tohoku.ac.jp
N
N
Hirasawa
H
H
Kagechika
K
K
Shudo
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K
Ohuchi
D000818:Animals; D000941:Antigens / Q000276:immunology*; D001565:Benzoates / Q000494:pharmacology; D002460:Cell Line; D016572:Cyclosporine / Q000494:pharmacology; D003984:Dibenzazepines / Q000494:pharmacology; D007166:Immunosuppressive Agents / Q000494:pharmacology; D015847:Interleukin-4 / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology*; D051381:Rats; D018168:Receptors, Retinoic Acid / Q000819:agonists / Q000037:antagonists & inhibitors / Q000502:physiology*; D047488:Retinoid X Receptors; D012176:Retinoids / Q000494:pharmacology*; D013764:Tetrahydronaphthalenes / Q000494:pharmacology; D014157:Transcription Factors / Q000819:agonists / Q000502:physiology
D000941:Antigens; D001565:Benzoates; D003984:Dibenzazepines; D007166:Immunosuppressive Agents; C104183:LE 540; D018168:Receptors, Retinoic Acid; D047488:Retinoid X Receptors; D012176:Retinoids; D013764:Tetrahydronaphthalenes; D014157:Transcription Factors; C061133:tamibarotene; C085268:Re 80; C104181:HX 600; D015847:Interleukin-4; D016572:Cyclosporine