Differences between coding and non-coding regions in the Trichomonas vaginalis genome: an actin gene as a locus model(1).
The sequence of a cloned genomic fragment of Trichomonas vaginalis containing a complete actin gene was determined. An uninterrupted open reading frame of 1128 nucleotides was found that codes for an actin gene. Two overlapped consensus promoter sequences for T. vaginalis were found 12 nucleotides upstream the actin initiation codon. In addition to actin, two incomplete open reading frames were found at the 5' and 3' ends of the clone. These two sequences are expressed and showed similarity to adenylate cyclase genes and a yeast hypothetical protein. The overall sequence showed a higher G+C content and a lower frequency of repeated sequences in the coding regions when compared with the non-coding regions. A similar unequal nucleotide distribution was found in various T. vaginalis genes retrieved from data bases.
Departamento de Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Apartado postal 70-228, CP 04510, México D.F., Mexico.
N
N
Espinosa
R
R
Hernández
L
L
López-Griego
R
R
Arroyo
I
I
López-Villaseñor
D000199:Actins / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D015342:DNA Probes / Q000737:chemistry; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D015723:Gene Library; D018503:Genome, Protozoan; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D016053:RNA, Protozoan / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D012091:Repetitive Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D014246:Trichomonas vaginalis / Q000737:chemistry / Q000235:genetics*
D000199:Actins; D015342:DNA Probes; D016054:DNA, Protozoan; D016053:RNA, Protozoan