Hair cell regeneration and recovery of auditory thresholds following aminoglycoside ototoxicity in Bengalese finches.
Birds regenerate auditory hair cells when original hair cells are lost. Regenerated hair cells become innervated and restore hearing function. Functional recovery during hair cell regeneration is particularly interesting in animals that depend on hearing for vocal communication. Bengalese finches are songbirds that depend on auditory feedback for normal song learning and maintenance. We examined the structural and functional recovery of the Bengalese finch basilar papilla after aminoglycoside ototoxicity. Birds were treated with the ototoxic aminoglycoside, amikacin, daily for 1 week. Treatment resulted in hair cell loss across the basal half of the basilar papilla and corresponding high frequency hearing loss. Hair cell regeneration and recovery of auditory brainstem responses were compared in the same animals. Survival times following treatment were between 1 day and 12 weeks. Analysis of structural recovery at weekly intervals indicated that hair cells in the Bengalese finch papilla require a maximum of 1 week to regenerate and appear with immature morphology at the epithelial surface. An additional 6 days are required for adult-like morphology to develop. Repopulation of the damaged region was complete by 8 weeks. Recovery of auditory thresholds began 1 week after treatment and reached asymptote by 4 weeks. Slight residual threshold shifts at 2.0 kHz and above were observed up to 12 weeks after treatment. Direct comparison of structural and functional recovery indicates that auditory thresholds recover maximally before a full complement of hair cells has regenerated.
Woolley
S M
SM
Neurobiology and Behavior Program, Department of Otolaryngology-HNS and Virginia Merrill Bloedel Hearing Research Center, P.O. Box 357923, University of Washington, Seattle, WA 98195, USA.
Wissman
A M
AM
Rubel
E W
EW
D000583:Amikacin / Q000633:toxicity*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D001309:Auditory Threshold / Q000187:drug effects; D006198:Hair Cells, Auditory / Q000187:drug effects* / Q000502:physiology* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D012038:Regeneration; D020308:Songbirds / Q000502:physiology*; D013997:Time Factors; D014828:Vocalization, Animal / Q000502:physiology
D000900:Anti-Bacterial Agents; D000583:Amikacin