[Correlation between human papillomavirus type and grading of cervical intraepithelial neoplasia].
OBJECTIVE
To disclose the correlation between human papillomavirus (HPV) type and grade of cervical intraepithelial lesion (CIN).


METHODS
PCR and in situ hybridization (ISH) techniques were used to detect the presence of HPV 6B/11, 16, 18 in 61 cases of CIN and 12 cases of cervical squamous cell carcinoma (SCC).


RESULTS
PCR showed HPV 6, 11 distributing mainly in low-grade squamous intraepithelial lesion (LSIL, 61.9%) and partly in CINII (20%), but not in CINIII and SCC. The frequency of HPV 16, 18 increased corresponding to the increasing of CIN grading and up to 83.3% in SCC. ISH showed HPV 6b/11, 16, 18 DNA hybridization intranuclear signal were diffusely distributed in LSIL and granular in appearance in episomal HPV. Similar signal were also seen in CINII infected by HPV 6b/11 and partly by HPV 16, 18, anyhow, CINIII, SCC and partly CINII HPV 16, 18 DNA hybridization were nongranular in appearance.


CONCLUSIONS
low-grade SIL associated with "low-risk" types of HPV 6, 11 belonged to productive infection. CINIII and SCC associated with "high-risk" types of HPV 16, 18 belonged to integrated infection, while CINII belonged to either productive or integrated infection.
Department of Pathology, Beijing Friendship Hospital, Capital University of Medical Sciences, Beijing 100050.
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Huang
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Zhang
D002294:Carcinoma, Squamous Cell / Q000821:virology; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D027383:Papillomaviridae / Q000145:classification / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000821:virology; D002578:Uterine Cervical Dysplasia / Q000821:virology*