Supercritical fluid extraction of polycyclic aromatic hydrocarbons from liver samples and determination by HPLC-FL.
An extraction/clean-up procedure by SFE was developed for isolating PAHs from liver samples for subsequent HPLC-FL determination of ten PAHs in the enriched extract. Recoveries (90-115%) and RSD % (< or =7.7) were satisfactory. When applied to 11 samples of bird of prey (Tyto alba) protected species and classified of special interest, from the Galicia (Northwest to Spain), benzo[ghi]perylene and indeno[1,2,3-cd]pyrene were undetectable; chrysene and benzo[a]pyrene are only detected in one sample; benzo[a]anthracene and benzo[k]fluoranthene are only quantified in one sample and benzo[b]fluoranthene in two samples. The other PAHs, anthracene, fluoranthene and pyrene are present in almost all the samples.
González Amigo
S
S
Department of Analytical Chemistry, Nutrition and Bromatology, University of Santiago de Compostela, Spain.
García Falcón
M S
MS
Lage Yusty
M A
MA
Simal Lozano
J
J
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008099:Liver / Q000737:chemistry*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis* / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D013030:Spain; D020305:Strigiformes
D011084:Polycyclic Aromatic Hydrocarbons