Development and application of the serological assay for humoral immune response against duck hepatitis B virus.
OBJECTIVE
To develop a simple and specific assay for detection of humoral immune response in duck hepatitis B virus (DHBV) infected ducks.


METHODS
Eighty serum samples were detected by ELISA for DHBsAg with prepared anti-preS 1H1 ascitic fluid and by PCR or dot blot hybridization for DHBV DNA. Thirty-two serum samples from experimentally infected 1-day-old ducks were assayed by ELISA for DHBcAb and DHBsAb.


RESULTS
Of 66 PCR positive samples, 58 were positive for DHBsAg and 62 were positive for DHBV dot blot hybridization. Sensitivities of the two methods were 87.9% and 93.9%, respectively. Anti-DHBc was developed in 2/8 infected ducks at day 21 but negative after day 28. Anti-DHBs antibodies were negative throughout the infectious period.


CONCLUSIONS
The application of DHBV infection and serological assay will be helpful to the study of kinetics of DHBV and humoral immune response of ducks against the virus.
The Research Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China.
N
N
Tang
A
A
Huang
S
S
Guo
D
D
Zhang
D000818:Animals; D004279:DNA, Viral / Q000032:analysis; D004372:Ducks; D004797:Enzyme-Linked Immunosorbent Assay; D006510:Hepatitis B Antibodies / Q000097:blood*; D006514:Hepatitis B Surface Antigens / Q000276:immunology; D015611:Hepatitis B Virus, Duck / Q000276:immunology*; D016133:Polymerase Chain Reaction
D004279:DNA, Viral; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens