[Msp I and Rsa I RFLPs of vWF gene in Chinese Han people].
OBJECTIVE
To study the Msp I and Rsa I restriction fragment length polymorphisms (RFLPs) in the 5' region of human von Willebrand factor gene in Chinese Han people.


METHODS
A 226 bp amplified fragment of human vWF gene from 52 unrelated individuals and a 322 bp amplified fragment from 48 individuals were analyzed by Msp I or Rsa I digestion.


RESULTS
Complete linkage disequilibrium was observed at both Msp I and Rsa I sites in vWF gene in Chinese Han people. The frequencies at the two sites were 0.20/0.80 for Msp I+/Msp I- and 0.93/0.07 for Rsa I+/Rsa I-. The theoretical heterozygous rates were 36% and 10%, respectively.


CONCLUSIONS
The Msp I and Rsa I RELPs of vWF gene are useful for genetic counseling and prenatal diagnosis of vWD in Chinese Han people.
Li
Z
Z
Thrombosis and Haemostasis Research Unit, Jiangsu Institute of Hematology, First Affiliated Hospital, Suzhou Medical College, Suzhou 215004.
Wan
H
H
Tai
H
H
D000483:Alleles; D044466:Asian People; D005006:Ethnicity; D006801:Humans; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D014842:von Willebrand Diseases / Q000235:genetics*; D014841:von Willebrand Factor / Q000235:genetics*
D014841:von Willebrand Factor