The sex steroid binding protein (SBP or SHBG) of human plasma: identification of Tyr-57 and Met-107 in the steroid binding site.
Tyrosine-57 (Y57) and methionine-107 (M107) have been identified in the binding site of the sex steroid binding protein (SBP) (or sex hormone binding globulin) of human plasma by replacing the two amino acids with a number of residues of varying structure. Replacement of Y57 with phenylalanine resulted in a fourfold increase in the K(d) of 5 alpha-dihydrotestosterone but left the K(d) of 17 beta-estradiol unchanged. Except in two cases, no further loss in binding took place when replacing Y57 with other residues, suggesting that the phenolic group of Y57 may form a hydrogen bond with the ligand. Replacement of M107 with isoleucine increased the 5 alpha-dihydrotestosterone K(d) fourfold to a value equal to that of rabbit SBP, which contains isoleucine at the corresponding position; however, the K(d) of 17 beta-estradiol remained unchanged. Replacement of M107 with threonine resulted in a tenfold decrease in 5 alpha-dihydrotestosterone binding affinity, whereas replacement with leucine left the K(d) unchanged. These data indicate that substitutions on the beta-carbon of the amino acid side-chain at position 107 causes significant loss of binding affinity but, as in the case of Y57, the activity was not totally eliminated. We conclude that Y57 and M107 form part of a structural motif within the steroid binding site and specifically contribute binding energy to ring A of 5 alpha-dihydrotestosterone but not to ring A of 17 beta-estradiol. We also propose that the integrated contribution of several side chains may be required to optimize the ligand affinity of the steroid binding site. This proposal may fit a 'lock and key' model where little movement of the side chains occurs during binding as might be expected for a rigid structure like the steroid nucleus.
Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.
P H
PH
Pétra
K T
KT
Woodcock
W R
WR
Orr
D K
DK
Nguyen
L M
LM
Sui
D019943:Amino Acid Substitution; D000818:Animals; D001665:Binding Sites; D019556:COS Cells; D016895:Culture Media, Serum-Free; D013196:Dihydrotestosterone / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D016297:Mutagenesis, Site-Directed; D011485:Protein Binding; D012738:Sex Hormone-Binding Globulin / Q000737:chemistry* / Q000378:metabolism*; D014162:Transfection
D016895:Culture Media, Serum-Free; D012738:Sex Hormone-Binding Globulin; D013196:Dihydrotestosterone; D004958:Estradiol