Effect of gonadotrophins on the ovarian interstitial tissue of the wood mouse, Apodemus sylvaticus.
The ovarian interstitial tissue of the wood mouse (Apodemus sylvaticus) is extensively developed. The effect of equine chorionic gonadotrophin (eCG) and human chorionic gonadotrophin (hCG) on ovarian interstitial tissue was investigated in wood mice from a laboratory stock. The tissue was assessed by measuring the relative size of the cells, cell nucleus diameter and (in adults) nuclear shape. Hormone-treated wood mice had larger interstitial cells and larger cell nuclei than untreated animals. In addition, the cell nuclei of adult hormone-treated wood mice had a smooth round or oval outline, whereas those of untreated animals had an irregular outline with spiky projections. Electron microscopy showed that the irregular spiky outline of the cell nuclei in untreated wood mice was caused by distortions of the nuclear membrane by a large number of intracellular lipid droplets; the droplets were less abundant in the hormone-treated animals. These experiments indicate that the cells of the interstitial tissue of the wood mouse are under the control of gonadotrophins, and that these cells are likely to be a site of the synthesis and release of steroid hormones. The methods used in this study to assess the state of the cells could be applied to animals from the field to investigate the role of interstitial tissue in the reproductive biology of wood mice.
Clarke
J
J
Department of Zoology, Oxford University, South Parks Road, Oxford OX1 3PS, UK. john.clarke@zoo.ox.ac.uk
Brook
F
F
D000375:Aging; D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D005260:Female; D008854:Microscopy, Electron; D009115:Muridae; D009685:Nuclear Envelope / Q000187:drug effects / Q000648:ultrastructure; D010053:Ovary / Q000187:drug effects* / Q000648:ultrastructure
D006063:Chorionic Gonadotropin