A note on the analysis of ligand binding by the 'double-logarithmic' plot.
The double-logarithmic plot has been employed for the fluorometric analysis of ligand binding. This analysis is valid only for 1:1 binding of a non-aggregating ligand and if the plot is based on the free, and not the total, ligand concentration.
Grossweiner
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Wenske Laser Center, Advocate Ravenswood Hospital Medical Center, Chicago, IL 60640, USA. lgflash@aol.com
D000818:Animals; D001665:Binding Sites; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008024:Ligands; D046911:Macromolecular Substances; D008956:Models, Chemical; D010777:Photochemistry; D011166:Porphyrins / Q000737:chemistry / Q000378:metabolism; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism; D013050:Spectrometry, Fluorescence
D008024:Ligands; D046911:Macromolecular Substances; D011166:Porphyrins; D012709:Serum Albumin