[Effect of hyperbaric oxygen on the expression of proteins Bcl-2 and Bax in the gerbil hippocampus CA1 following forebrain ischemia reperfusion].
OBJECTIVE
To study the mechanisms of how hyperbaric oxygen treatment (HBOT) reduced neuronal apoptosis following forebrain ischemia reperfusion.


METHODS
Changes of the expression of protein Bcl-2 or Bax were observed in CA1 region of gerbil hippocampus following HBOT on ischemia reperfusion 3 days using the method of labelled streptavidin biotin (LSAB) immunohistochemistry staining. For this purpose gerbils were exposed to the 0.15 MPa and 0.25 MPa HBO 60 min every day for 3 successive days.


RESULTS
The expression of protein Bcl-2 in hippocampus CA1 was significantly increased in HBOT groups (P < 0.01), and changes in 0.25 MPa groups were greater than those in 0.15 MPa groups (P < 0.01). The expression of protein Bax in hippocampus CA1 was not changed significantly in HBOT groups.


CONCLUSIONS
HBO can induce the expression of Bcl-2, which is the mechanism of neuronal protecting effect of HBOT.
Naval Medical Research Institute of CPLA, Shanghai 200433.
J G
JG
Zhou
J C
JC
Liu
Y Q
YQ
Fang
D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism* / Q000628:therapy; D005260:Female; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D006931:Hyperbaric Oxygenation; D008297:Male; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis*; D051381:Rats; D015427:Reperfusion Injury / Q000378:metabolism* / Q000628:therapy; D051028:bcl-2-Associated X Protein
C494828:Bax protein, rat; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein