Biomarkers of DNA damage in patients with end-stage renal disease: mitochondrial DNA mutation in hair follicles.
BACKGROUND
DNA damage was noted in patients with end-stage renal disease (ESRD). Mitochondrial DNA (mtDNA) mutations have been proposed as a genomic biomarker in the process of human ageing, degenerative diseases and carcinogenesis.


METHODS
Polymerase chain reaction (PCR) techniques were applied to detect mtDNA deletions in hair follicles, an appendage of skin, from 162 patients with ESRD.


RESULTS
The incidences of the 4977 bp deletion of mtDNA in hair follicles were found to increase with age in normal control and ESRD patients. As compared with normal subjects, ESRD patients had 3.5, 2.3, 2.7, 2.3 and 1.4 times higher incidences of the 4977 bp deletion of mtDNA in the age groups of 20-30, 31-40, 41-50, 51-60 and 61-70 years, respectively. Moreover, the difference in the proportion of mtDNA with the 4977 bp deletion was statistically significant between ESRD patients and normal subjects >50 years of age.


CONCLUSIONS
We suggest that the 4977 bp deletion of mtDNA in hair follicles may serve as one of the tissue biomarkers of genetic instability of the mitochondrial genome in ESRD patients.
Department of Internal Medicine, Kuang Tien General Hospital, Taichung, Taiwan.
C S
CS
Liu
L Y
LY
Ko
P S
PS
Lim
S H
SH
Kao
Y H
YH
Wei
D000328:Adult; D000368:Aged; D015415:Biomarkers; D004249:DNA Damage; D004272:DNA, Mitochondrial / Q000235:genetics*; D017353:Gene Deletion; D018859:Hair Follicle / Q000503:physiopathology*; D006801:Humans; D007676:Kidney Failure, Chronic / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D009154:Mutation
D015415:Biomarkers; D004272:DNA, Mitochondrial