[Both myeloma cells and bone marrow stromal cells are involved in the overproduction of interleukin 6 in multiple myeloma].
OBJECTIVE
To investigate the mechanism of interleukin 6 (IL-6) overexpression in multiple myeloma(MM).


METHODS
Human myeloma cells(KM3) and bone marrow stromal cells(BMSCs) were fixed by paraformaldehyde. KM3 cells and BMSCs (fixed and non-fixed) were co-cultured in 10%FCS-RPMI1640. IL-6 activity in the supernatant was determined by B9 cell proliferation test.


RESULTS
Both non-fixed myeloma cells and BMSCs could automatically secret IL-6, while the fixed cells did not. Co-cultures of fixed one with non-fixed the other of either kind of the cells could significantly increase the IL-6 activity in the supernatants. The promoting effect on IL-6 production was greater in BMSCs from MM patients than that in BMSCs from normal controls.


CONCLUSIONS
There exists dual mechanism of IL-6 overproduction in MM, i.e. both autocrine and paracrine of IL-6 are involved in the pathogenesis of MM.
Changzheng Hospital, 2nd Military Medical University, Shanghai 200003.
S
S
Ding
J
J
Hou
A
A
Sun
D001854:Bone Marrow Cells / Q000378:metabolism*; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000378:metabolism*; D017154:Stromal Cells / Q000378:metabolism
D015850:Interleukin-6