Activity and mRNA abundance of Delta-5 and Delta-6 fatty acid desaturases in two human cell lines.
We analyzed fatty acid biosynthesis in Chang and ZR-75-1 cells. Both cell lines could desaturate and further elongate substrates for Delta-5 desaturase. ZR-75-1 but not Chang cells showed Delta-6 desaturation of 18:2n-6, 18:3n-3, 24:4n-6 and 24:5n-3. In both cell lines, the mRNA abundance can be related to Delta-5 or Delta-6 fatty acid desaturase activities. These results suggest that desaturase genes could have, at least in part, independent control mechanisms and that Delta-6 desaturase impairment is not specific to any particular step of the fatty acid metabolic pathways, which may diminish the rationale for the existence of at least two distinct enzymes.
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R J
RJ
de Antueno
S J
SJ
Allen
A
A
Ponton
M D
MD
Winther
D001943:Breast Neoplasms / Q000201:enzymology*; D002458:Cell Fractionation; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D000090943:Delta-5 Fatty Acid Desaturase; D044943:Fatty Acid Desaturases / Q000235:genetics / Q000378:metabolism*; D015525:Fatty Acids, Omega-3 / Q000378:metabolism; D043371:Fatty Acids, Omega-6; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D050602:Linoleoyl-CoA Desaturase; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D012333:RNA, Messenger / Q000378:metabolism*
D000090943:Delta-5 Fatty Acid Desaturase; D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6; D005231:Fatty Acids, Unsaturated; D012333:RNA, Messenger; D044943:Fatty Acid Desaturases; D050602:Linoleoyl-CoA Desaturase