Modulation of functional activities of chicken heterophils by recombinant chicken IFN-gamma.
The objective of the present studies was to examine the in vitro effects of recombinant chicken interferon-gamma (rChIFN-gamma) on shape change, phagocytosis, and the oxidative/nonoxidative killing activities of day-old chicken heterophils. Heterophils (4 x 10(6)/ml) were incubated with various concentrations of recombinant ChIFN-gamma from both Escherichia coli and transfected Cos cells for 2 h at 39 degrees C. The incubation of the neonatal heterophils with rChIFN-gamma resulted in significantly greater numbers of cells with membrane shape change when compared with the mock-treated heterophils. Both Cos cell-derived and E. coli-derived ChIFN-gamma significantly increased (p < 0.01) the phagocytosis of opsonized or nonopsonized Salmonella enteritidis by the neonatal heterophils in a concentration-dependent manner. Incubation with ChIFN-gamma induced no direct stimulation of the respiratory burst by the chicken heterophils but did prime the heterophils for a significantly strengthened respiratory burst to subsequent stimulation with opsonized zymosan (OZ). Lastly, incubation of the heterophils with ChIFN-gamma primed the cells for a significant increase in the release of beta-D-glucuronidase following stimulation with OZ. These results show that neonatal avian heterophils can respond to cytokine modulation with enhanced functional competence, suggesting that ChIFN-gamma can enhance the immune competence of the innate defenses of chickens during the first week of life.
Kogut
M H
MH
USDA-Agricultural Research Service, Southern Plains Agricultural Research Center, College Station, TX 77845, USA. kogut@ffsru.tamu.edu
Pishko
E J
EJ
Kaspers
B
B
Weining
K C
KC
D000818:Animals; D000831:Animals, Newborn; D019556:COS Cells; D015550:Cell Degranulation / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D002645:Chickens; D004926:Escherichia coli / Q000235:genetics; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000235:genetics / Q000494:pharmacology*; D007962:Leukocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D008163:Luminescent Measurements; D010084:Oxidation-Reduction; D010587:Phagocytosis / Q000187:drug effects; D011994:Recombinant Proteins; D016897:Respiratory Burst / Q000187:drug effects; D014162:Transfection; D015054:Zymosan / Q000494:pharmacology
D011994:Recombinant Proteins; D007371:Interferon-gamma; D015054:Zymosan