Microparticle-mediated RNA immunization against bovine viral diarrhea virus.
Infectious transcripts from the full-length infectious clone of the NADL strain of bovine viral diarrhea virus (BVDV) were used to vaccinate cattle and sheep against BVDV. In vitro synthesized RNA delivered by microparticle bombardment with a Helios Gene Gun initiated replication of BVDV and consequently induced humoral immunity against type I BVDV (serum neutralization titers, SNT > 2(12)) and type II BVDV (SNT > 2(7)). The quality and long-term stability of the RNA-carrier complexes was assessed by microparticle bombardment of tissue culture monolayers. The RNA cartridges were found to be stable for at least 8 months upon storage. This is the first report on successful RNA vaccination of large ruminants.
Department of Veterinary and Biomedical Sciences, University of Nebraska, Lincoln 68583-0905, USA.
V B
VB
Vassilev
L H
LH
Gil
R O
RO
Donis
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D019470:Biolistics; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000276:immunology / Q000517:prevention & control; D002417:Cattle; D002478:Cells, Cultured; D001908:Diarrhea Viruses, Bovine Viral / Q000235:genetics* / Q000276:immunology*; D008863:Microspheres; D020871:RNA Stability; D012367:RNA, Viral / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology*; D012756:Sheep; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology*
D000914:Antibodies, Viral; D012367:RNA, Viral; D014614:Vaccines, Synthetic; D014765:Viral Vaccines