Activation of antigen-specific T cells by artificial cell constructs having immobilized multimeric peptide-class I complexes and recombinant B7-Fc proteins.
T cell activation results from the engagement of multiple receptors on T cells by their respective ligands on antigen presenting cells. Studies using artificial cell surface constructs have demonstrated that effective T cell response requires that antigen be presented on a solid surface with dimensions that approximate those of an intact cell. In this report, we describe the cloning and expression of recombinant B7-1-Fc and B7-2-Fc proteins and their incorporation onto 5-microm latex microspheres along with renatured peptide-MHC. These microspheres provide a simple and effective method for the in vitro or in vivo stimulation of antigen-specific T cells under precisely controlled antigen and costimulation conditions.
Center for Immunology and Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN 55455, USA.
E L
EL
Tham
P L
PL
Jensen
M F
MF
Mescher
D000818:Animals; D017951:Antigen Presentation; D018122:B7-1 Antigen / Q000235:genetics / Q000276:immunology*; D018914:Cells, Immobilized / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D007141:Immunoglobulin Fc Fragments / Q000235:genetics / Q000276:immunology*; D007158:Immunologic Techniques; D008213:Lymphocyte Activation; D051379:Mice; D008822:Mice, Transgenic; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D018122:B7-1 Antigen; D015395:Histocompatibility Antigens Class I; D007141:Immunoglobulin Fc Fragments; D011993:Recombinant Fusion Proteins