Gestational age-dependent effects of lipopolysaccharide on prostaglandin production by murine decidual caps.
We sought to determine whether the gestational age of the pregnant mouse had any relationship with its lipopolysaccharide (LPS) responsiveness. Murine decidual caps from days 13, 15 and 17 of gestation (term is day 20) were dissected out, placed in inserts and equilibrated in media overnight. The following day, media were removed, replaced with fresh media (+/-LPS at 10 microg/mL). After LPS stimulation (24 h), prostaglandin (PG)E2 production by decidual caps from days 13 and 15 increased by 80-fold and 5-fold, respectively. PGF2alpha, 6-keto-PGF1a and TxB2 production also increased. Day 17 decidual caps were unaffected by LPS, pregnant mice inoculated i.p. with LPS (50 microg) at day 13 of gestation induced 100% delivery within 24 h. However, mice treated at days 15 and 17 had an equal occurrence of premature delivery or fetal resorption. This change in LPS responsiveness may indicate changes in the fetal-maternal immune system in late pregnancy.
Liggins Institute and Division of Pharmacology and Clinical Pharmacology, Faculty of Medical and Health Sciences, University of Auckland, Auckland, New Zealand. t.sato@auckland.ac.nz
T A
TA
Sato
J A
JA
Keelan
D K
DK
Gupta
M D
MD
Mitchell
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D000818:Animals; D046508:Culture Techniques; D003656:Decidua / Q000378:metabolism; D004622:Embryo, Mammalian / Q000378:metabolism*; D005260:Female; D005865:Gestational Age; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D008809:Mice, Inbred C3H; D011453:Prostaglandins / Q000096:biosynthesis*; D011863:Radioimmunoassay; D013997:Time Factors
D008070:Lipopolysaccharides; D011453:Prostaglandins; D015121:6-Ketoprostaglandin F1 alpha