Molecular characterization of Salmonella weltevreden isolated from poultry: evidence of conjugal transfer of plasmid and antibiotic resistance.
Ten strains of Salmonella weltevreden isolated from poultry sources were examined and found to contain plasmid DNA ranging in size from 1.8 to 68.5 MD. All isolates were susceptible to carbenicillin, cephalothin, ceftriazone, gentamicin, kanamycin and nalidixic acid, but resistance to bacitracin (100%), penicillin G (100%), rifampicin (100%), sulphamethoxazole (100%), cefuroxime (80%) and tetracycline (60%) was recorded. The 55 MD plasmid of strain SW5 determined resistance to penicillin G and tetracycline, which was transmissible to the E. coli K12 recipient at a frequency of 3.52 x 10(-5) transconjugants per input donor cell. The results of arbitrarily primed polymerase chain reaction (AP-PCR), using two 10-mer oligonucleotides and PCR-ribotyping to differentiate between the ten strains of S. weltevreden were compared. The strains were separated into ten different genome types by AP-PCR but were indistinguishable by PCR-ribotyping. These results suggest that poultry may constitute a reservoir for disseminating antibiotic resistance and that AP-PCR may be a valuable tool for epidemiological studies.
Department of Biotechnology, Faculty of Food Science and Biotechnology, University Putra Malaysia, Selangor.
S
S
Radu
S A
SA
Mutalib
G
G
Rusul
Z
Z
Hassan
L K
LK
Yeang
D000818:Animals; D003227:Conjugation, Genetic / Q000235:genetics*; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry; D004352:Drug Resistance, Microbial / Q000235:genetics; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011200:Poultry; D011201:Poultry Diseases / Q000175:diagnosis / Q000382:microbiology*; D011815:R Factors / Q000737:chemistry / Q000235:genetics*; D021521:Ribotyping / Q000662:veterinary; D012475:Salmonella / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D012481:Salmonella Infections, Animal / Q000175:diagnosis / Q000382:microbiology*
D017931:DNA Primers; D004269:DNA, Bacterial