[Application of fluorescence in situ hybridization (FISH) in sputum cytologic diagnosis of lung cancer].
OBJECTIVE
To study the numerical chromosomal abnormalities of cells in sputum from patients with lung cancer by dual-fluorescence in situ hybridization (FISH).


METHODS
Thirty sputum samples from lung cancer patients were examined by FISH with centromere DNA probes of chromosome 7, 11, 17 and X.


RESULTS
In 23 positive sputum samples studied, the frequency of hyperdiploid of chromosome 7, 17, X and 11 was 65.2% (15/23), 60.9% (14/23), 52.2% (12/23) and 39.1% (9/23), respectively. Hyperdiploidy of chromosome 7 was found in 4 of 7 sputum samples which were cytologicaly suspicious of cancer cells.


CONCLUSIONS
FISH can detect aneuploid malignant cells in sputum and be used as a complementary technique to cytologic diagnosis of lung cancer.
Cancer Institute (Hospital), Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100021, China.
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D
Jia
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Z
Zhang
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Liu
D000230:Adenocarcinoma / Q000175:diagnosis* / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000235:genetics; D004171:Diploidy; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000235:genetics; D008297:Male; D013183:Sputum / Q000166:cytology*