Somatotropin has no effect on the quantity of guanine nucleotide binding proteins Gqalpha/G11alpha in goat adipose tissue in vivo.
The decapeptide QLNLKEYNLV corresponding to the C-terminus of Gq/G11alpha guanine nucleotide-binding proteins (G-proteins) was synthesized by the solid-phase method and conjugated to keyhole limpet hemocyanin. The rabbits were immunized with these conjugates and an antiserum that reacted specifically with the alpha subunit of Gq/G11 proteins was used in this study. The antiserum exhibited no cross-reactivity with the alpha subunits of stimulatory (Gs) or inhibitory (Gi) G-proteins associated with adenylate cyclase. Immunoblots with the antiserum showed that it specifically recognized the Gq/G11 alpha-proteins in cholate extracts of adipose tissue membranes of goats. Treatment of young castrated male goats with bST had no effect on the quantity of Gq/G11 alpha subunits in adipose tissue and the results thus obtained did not support the idea that the bST signal in adipose tissue is transmitted via Gq/G11 alpha-proteins.
Krbecek
V
V
Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Prague.
Kovarů
H
H
Skarda
J
J
Barth
T
T
Velek
J
J
Zizkovský
V
V
D000273:Adipose Tissue / Q000737:chemistry* / Q000187:drug effects* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D015153:Blotting, Western; D006041:Goats / Q000378:metabolism*; D013006:Growth Hormone / Q000494:pharmacology*; D020962:Heterotrimeric GTP-Binding Proteins / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D007106:Immune Sera / Q000096:biosynthesis / Q000276:immunology; D008297:Male; D009919:Orchiectomy; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D011817:Rabbits; D010738:Type C Phospholipases / Q000378:metabolism
D007106:Immune Sera; D010446:Peptide Fragments; D013006:Growth Hormone; D010738:Type C Phospholipases; D020962:Heterotrimeric GTP-Binding Proteins