Spontaneous inflammatory disease in HLA-B27 transgenic mice does not require transporter of antigenic peptides.
HLA-B27 is strongly linked with a group of human diseases called spondyloarthropathies. Even though HLA-B27 as an MHC class I molecule would be expected to present endogenously processed peptides such as cytosolic or viral proteins, many of the B27-linked diseases begin after an infection with an enterobacteria, an exogenous antigen. In our previous studies, we have described development of spontaneous inflammatory disease in HLA-B27 transgenic mice expressing beta(2)m free heavy chains on the cell surface. In order to address the role of endogenous versus exogenous antigens and a role for Tap genes in the development of spontaneous diseases, mice lacking Tap-1 (knockout) were mated to HLA-B27/human beta(2)m transgenic mice. B27(+)/human beta(2)m(+) double-transgenic mice (without mouse beta(2)m) lacking the Tap-1 gene developed spontaneous inflammatory disease similar to wild-type Tap-1 gene-expressing counterparts. Our data demonstrate that peptide transporters (Tap) were not involved in the development of spontaneous inflammatory disease in B27(+)/human beta(2)m transgenic animals.
Khare
S D
SD
Department of Immunology, Mayo Clinic and Medical School, Rochester, Minnesota, 55905, USA.
Lee
S
S
Bull
M J
MJ
Hanson
J
J
Luthra
H S
HS
David
C S
CS
D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D018528:ATP-Binding Cassette Transporters; D000818:Animals; D016326:Extracellular Matrix Proteins / Q000502:physiology*; D015796:HLA-B27 Antigen / Q000502:physiology*; D007249:Inflammation / Q000209:etiology*; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000502:physiology*; D011110:Polymorphism, Genetic; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis; D001613:beta 2-Microglobulin / Q000032:analysis
D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D018528:ATP-Binding Cassette Transporters; D016326:Extracellular Matrix Proteins; D015796:HLA-B27 Antigen; D009419:Nerve Tissue Proteins; D016693:Receptors, Antigen, T-Cell, alpha-beta; C073119:TAP1 protein, human; D001613:beta 2-Microglobulin