[The regulating mechanism of anti-fungicides on mouse oocyte development].
OBJECTIVE
To study the mechanism of the effect of gonadotropin-induced oocyte maturation.


METHODS
Mouse oocytes were cultured in HX-medium, and the effects of amphotericin B and ketoconazole on resumption of meiosis of mouse oocyte were examined.


RESULTS
1. FSH(10-200 IU/L) induced a dose-dependent manner of oocytes maturation in CEO. A maximum increase in GVBD was observed with 25-50 IU/L FSH. 2. Amphotericin tericim B (0.025-2.5 micrograms/L) caused significant increases in GVBD in CEO, which mimicked the function of FSH. 3. Ketoconazole (10(-7)-10(-3) mol/L) inhibited the effect of FSH on resumption of meiosis, but no effect on oocyte spontaneous maturation.


CONCLUSIONS
Amphotericin B and ketoconazole are able to affect mouse oocyte maturation, and indicates that they have a regulation on FSH-induced synthesis of meiosis-activating sterol.
College of Biological Sciences, China Agricultural University, Beijing 100094.
Z X
ZX
Lu
G L
GL
Xia
H B
HB
Wang
Y
Y
Cheng
M L
ML
Li
D000666:Amphotericin B / Q000494:pharmacology; D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000502:physiology; D066298:In Vitro Techniques; D007654:Ketoconazole / Q000494:pharmacology; D008540:Meiosis / Q000187:drug effects; D051379:Mice; D009865:Oocytes / Q000166:cytology / Q000187:drug effects*
D000935:Antifungal Agents; D000666:Amphotericin B; D005640:Follicle Stimulating Hormone; D007654:Ketoconazole