Gene expression profiling of malignant mesothelioma cell lines: cDNA array study.
To reveal genes relevant for malignant mesothelioma (MM), we carried out cDNA array experiments on 4 MM cell lines and 2 primary mesothelial cell cultures established from pleural fluid of non-cancer patients. Human cancer gene filters including 588 genes were used for the cDNA array experiments. Our study revealed 26 over-expressed genes that play a role in the regulation of cell fate, cell cycle, cell growth and DNA damage repair and 13 under-expressed genes encoding growth factors, receptors and proteins involved in cell adhesion, motility and invasion to be common to 3 or 4 MM cell lines. We confirmed the cDNA array results using RT-PCR for 5 of the over-expressed genes and for 3 of the under-expressed genes. Our study presents gene expression profiles in MM cell lines and shows the involvement of several genes, such as those encoding JAGGED1, ser/thr protein kinase NIK, Ku80 and cyclin D2, novel in MM.
Department of Medical Genetics, Haartman Institute and Helsinki University Central Hospital, University of Helsinki, FIN-00029 HUCH, Helsinki, Finland.
E
E
Kettunen
A M
AM
Nissén
T
T
Ollikainen
M
M
Taavitsainen
J
J
Tapper
K
K
Mattson
K
K
Linnainmaa
S
S
Knuutila
W
W
El-Rifai
D018076:DNA, Complementary / Q000378:metabolism*; D015536:Down-Regulation; D004587:Electrophoresis, Agar Gel; D004848:Epithelium / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008654:Mesothelioma / Q000235:genetics* / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
D018076:DNA, Complementary