Aminoglycoside antibiotics, neamine and its derivatives as potent inhibitors for the RNA-protein interactions derived from HIV-1 activators.
Neamine derivatives which have an arginine (RN), a pyrene (PCN) and both pyrene and arginine (PRN) have been prepared and their binding toward the RNA fragments derived from HIV-1 activator region, TAR and RRE RNA were examined. Among them, PRN bound either TAR RNA or RRE RNA with equivalent binding affinities as Tat and Rev peptide, respectively.
Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohamna, Japan. khamasak@bio.titech.ac.jp
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Hamasaki
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Ueno
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001665:Binding Sites; D015694:Gene Products, rev / Q000378:metabolism*; D015696:Gene Products, tat / Q000378:metabolism*; D015497:HIV-1 / Q000187:drug effects* / Q000378:metabolism; D008969:Molecular Sequence Data; D009355:Neomycin / Q000494:pharmacology*; D012367:RNA, Viral / Q000378:metabolism*; D054321:rev Gene Products, Human Immunodeficiency Virus; D054322:tat Gene Products, Human Immunodeficiency Virus
D000900:Anti-Bacterial Agents; D015694:Gene Products, rev; D015696:Gene Products, tat; D012367:RNA, Viral; D054321:rev Gene Products, Human Immunodeficiency Virus; D054322:tat Gene Products, Human Immunodeficiency Virus; D009355:Neomycin