Visualization of detergent insoluble cyclic AMP-dependent protein kinase RIalpha aggregates in the rat brain.
Regulatory subunits of the cAMP dependent protein kinases are the most abundant receptor for cAMP in eukaryotic cells. Four isoforms of regulatory subunits (RIalpha and -beta, RIIalpha and -beta) have been distinguished. Distribution of the most abundant RII isoforms has been extensively studied in the brain, by immunohistochemistry and biochemical fractionation, while the least abundant RI isoforms have been neglected. In neurons most regulatory subunits are bound to the cytoskeleton. A protocol is presented that allows immunohistochemical and biochemical characterization of detergent-insoluble RI isoforms in the brain.
Dipartimento di Anatomia e Fisiologia Umana, Università di Padova, Via Marzolo 3, 35131 Padua, Italy. mugicnat@ux1.unipd.it
C
C
Mucignat-Caretta
A
A
Caretta
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002458:Cell Fractionation / Q000379:methods; D017868:Cyclic AMP-Dependent Protein Kinases / Q000032:analysis*; D003902:Detergents; D007150:Immunohistochemistry / Q000379:methods*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012995:Solubility
D003902:Detergents; D017868:Cyclic AMP-Dependent Protein Kinases