[PTA1 monoclonal antibody induces human platelet aggregation and intra-cytoplasmic Ca2+ elevation].
OBJECTIVE
To explore the mechanism of PTA1 monoclonal antibody (McAb) induced human platelet aggregation and its effect on intra-cytoplasmic Ca2+ level.


METHODS
Platelet aggregation, ATP releasing assay and Pollock's test were used.


RESULTS
PTA1 McAb induced human platelet aggregation in vitro, which could be completely inhibited by EGTA and PGI2. F(ab')2 of PTA1 McAb had no effect on CD9 or CD41 induced platelet activation and aggregation. PTA1 McAb enhanced platelet intra-cytoplasmic Ca2+ elevation.


CONCLUSIONS
PTA1 McAb inducing platelet aggregation is related to platelet Fc receptor and CD41/CD61 (II b/III a) complex, and the induced platelet intra-cytoplasmic Ca2+ elevation is resulted from Ca2+ influx and releasing of intracytoplasmic Ca2+ storage.
Sun
C
C
Department of Immunology, Fourth Military Medical University, Xian 710032.
Jin
B
B
Liu
X
X
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics* / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D006801:Humans; D010974:Platelet Aggregation / Q000187:drug effects*; D011961:Receptors, Fc / Q000378:metabolism; D000098862:T Lineage-Specific Activation Antigen 1
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000098862:T Lineage-Specific Activation Antigen 1; D011961:Receptors, Fc; D002118:Calcium