The new hematology analyzer Sysmex XE-2100: performance evaluation of a novel white blood cell differential technology.
BACKGROUND
The new hematology analyzer Sysmex XE-2100 (TOA Medical Electronics, Kobe, Japan) has a novel, combined, white blood cell differential technology and a special reagent system to enumerate nucleated red blood cells.


METHODS
Performance evaluation of both technologies of the Sysmex XE-2100 according to the H20-A protocol of the National Committee for Clinical and Laboratory Standards and comparison of the results with those for the hematology analyzer Sysmex NE-8000 (TOA Medical Electronics).


METHODS
Five hundred forty-four blood samples randomly chosen from various inpatient and outpatient departments of the Vienna University hospital.


RESULTS
Five-part white blood cell differential counts on the XE-2100 revealed excellent correlation with the manual reference method for neutrophils, lymphocytes, and eosinophils (r =.925,.922, and.877, respectively) and good correlation for monocytes and basophils (r =.756 and.763, respectively). The efficiency rates of flagging for the presence of >/=1% abnormal white blood cells were 83% (XE-2100) and 66% (NE-8000). The correlation of automated and microscopic nucleated red blood cell counts was excellent (r =.97).


CONCLUSIONS
From the present evaluation and our former experience with other types of Sysmex analyzers, we conclude that the new white blood cell differential technology of the XE-2100 represents a further development toward more efficient flagging of abnormal white blood cells.
Ruzicka
K
K
Department of Laboratory Medicine, University of Vienna, Austria.
Veitl
M
M
Thalhammer-Scherrer
R
R
Schwarzinger
I
I
D001322:Autoanalysis / Q000592:standards*; D004900:Erythroblasts / Q000166:cytology; D004906:Erythrocyte Count / Q000295:instrumentation; D005188:False Negative Reactions; D005189:False Positive Reactions; D006405:Hematology / Q000295:instrumentation* / Q000592:standards; D006801:Humans; D007958:Leukocyte Count / Q000295:instrumentation / Q000592:standards*; D007962:Leukocytes / Q000473:pathology; D012680:Sensitivity and Specificity