Prolonged stability of endogenous cardiotrophin-1 in whole blood.
Cardiotrophin-1 (CT-1) is a recently identified cytokine of the interleukin-6 (IL-6) family that signals through the gp130 signalling pathway. CT-1 may be of central importance to the pathogenesis of ventricular remodelling in patients with acute myocardial infarction (AMI) and therefore have clinical value in the identification of patients with impaired ventricular function. Central to the clinical use of CT-1 is in the in vitro stability of the peptide. Twelve subjects were recruited. A total of 25 mL of peripheral venous blood was collected into chilled polypropylene tubes containing EDTA and aprotinin and divided into 5 aliquots. One sample was spun in a prerefrigerated centrifuge (4 degrees C) at 3,000 rpm for 10 minutes and plasma separated and frozen at -70 degrees C immediately. Remaining samples were stored for 24 and 48 hours at room temperature or on ice. CT-1 in extracted plasma specimens was measured with a competitive chemiluminescent assay. The concentration of CT-1 in samples stored optimally was 43.1 +/- 6.05 fmol/mL. CT-1 levels for storage at room temperature compared with ice at the remaining time points were as follows: 24 hours, 41.5 +/- 5.76 v 37.5 +/- 8.66; and 48 hours, 42.6 +/- 6.28 v 41.0 +/- 5.42 fmol/mL. There were no significant changes in concentrations of CT-1 stored optimally or kept for up to 48 hours in aliquots of whole blood at room temperature or on ice. We conclude that CT-1 is stable in specimens of whole blood treated with EDTA and aprotinin and stored for up to 48 hours at room temperature or on ice, hence permitting its development in the routine clinical investigation of patients with heart failure.
Department of Medicine and Therapeutics, University of Leicester, UK.
P F
PF
Downie
S
S
Talwar
I B
IB
Squire
J E
JE
Davies
L L
LL
Ng
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000595:Amino Acid Sequence; D000789:Angina, Unstable / Q000097:blood; D007611:Aprotinin; D001798:Blood Proteins / Q000032:analysis / Q000378:metabolism*; D001800:Blood Specimen Collection / Q000379:methods*; D016207:Cytokines / Q000032:analysis / Q000097:blood* / Q000737:chemistry / Q000378:metabolism*; D004492:Edetic Acid; D005260:Female; D006352:Heart Ventricles / Q000473:pathology; D006801:Humans; D007053:Ice; D007118:Immunoassay; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009203:Myocardial Infarction / Q000097:blood; D010446:Peptide Fragments / Q000032:analysis / Q000097:blood / Q000737:chemistry / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
D001798:Blood Proteins; D016207:Cytokines; D007053:Ice; D010446:Peptide Fragments; D007611:Aprotinin; D004492:Edetic Acid; C092073:cardiotrophin 1