Terminal differentiation of human promyelocytic leukaemia cells, HL-60, during the G1 period.
Human promyelocytic leukaemic cells, HL-60, arrested in mitosis by nocodazole were released in the presence of 1alpha,25-dihydroxyvitamin D3 and thymidine or hydroxyurea. Cells moved from early G1 period to the G1/S boundary and differentiated. Furthermore, cells arrested at the G1/S boundary by double thymidine block were released, with 1alpha,25-dihydroxyvitamin D3 being added at the end of DNA synthesis. Under the latter conditions, differentiated cells developed, indicating that DNA synthesis is not required for cell differentiation.
Department of Cell Biology, The Osaka Medical Center for Cancer and Cardiovascular Diseases, 1-3-3, Nakamichi, Higashinari, Osaka, 537-8511, Japan.
T
T
Matsuhisa
D002117:Calcitriol / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D004273:DNA, Neoplasm / Q000096:biosynthesis; D016193:G1 Phase; D018922:HL-60 Cells; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology; D015473:Leukemia, Promyelocytic, Acute / Q000378:metabolism / Q000473:pathology*; D015739:Nocodazole / Q000494:pharmacology; D016196:S Phase; D013936:Thymidine / Q000494:pharmacology
D004273:DNA, Neoplasm; D002117:Calcitriol; D015739:Nocodazole; D013936:Thymidine; D006918:Hydroxyurea