[Platelet-derived growth factor (PDGF) mRNA expression of porcine pulmonary artery smooth muscle cells induced by hypoxic endothelial cells conditioned medium].
OBJECTIVE
To study the effect of hypoxic endothelial cell conditioned medium on the autocrine of PDGF in porcine pulmonary artery smooth muscle cells (PASMC).


METHODS
Experimental groups included serum-free medium group (control group), normoxic endothelial cell conditioned medium (NECCM) group and hypoxic endothelial cell conditioned medium (HECCM) group. The expression of PDGF-A and -B chain mRNA was determined by in situ hybridization and image analysis.


RESULTS
A weak expression for PDGF-A and PDGF-B mRNA was observed in PASMC cultured in serum free medium (control group); HECCM markedly enhanced the expression of PDGF-A and PDGF-B mRNA in PASMC, both being about 1.8-fold and 1.73-fold of the control group respectively, and 1.4-fold and 1.5-fold of the NECCM group respectively (P < 0.01).


CONCLUSIONS
PDGF may be an autocrine growth factor of PASMC induced by HECCM and may play an important role in vascular structural remodeling of hypoxic pulmonary hypertension.
Key Laboratory of Cell Biology, Ministry of Public Health of China, China Medical University, Shenyang 110001.
F
F
Li
Y
Y
Zhang
D
D
Che
D000818:Animals; D015687:Cell Hypoxia; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D004730:Endothelium, Vascular / Q000166:cytology*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis* / Q000235:genetics; D020574:Proto-Oncogene Proteins c-sis / Q000096:biosynthesis* / Q000235:genetics; D011651:Pulmonary Artery / Q000166:cytology / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D013552:Swine
D017077:Culture Media, Conditioned; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger; C075278:platelet-derived growth factor A