Methemoglobinemia in aluminium phosphide poisoning in rats.
Aluminium phosphide (AlP) a grain fumigant is the leading cause of intentional poisoning in North India. The mechanisms involved in toxicity are not known and there is no antidote till date. The present study was carried out to investigate the oxygen free radical generation, methemoglobinemia and effect of methylene blue treatment on survival time in rat model of AlP poisoning. AlP (50 mg/kg, intragastric) was administered in one group and the other group received AlP + Methylene Blue (MB) (0.1%, 1 mg/kg/5 min, i.v.). Malonyldialdehyde (MDA) and methemoglobin (MeHb) levels were measured at 10 and 30 min intervals. Blood MDA levels increased at 10 and 30 min after AlP exposure with simultaneous rise in MeHb levels suggesting methemoglobinemia could be due to increased oxygen free radical generation. Methylene blue caused a significant fall in both the parameters with prolongation of survival time. It is concluded that AlP causes methemoglobinemia responding to methylene blue treatment.
Department of Pharmacology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, India.
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Lall
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Peshin
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Mitra
D017607:Aluminum Compounds / Q000506:poisoning*; D000818:Animals; D005260:Female; D005609:Free Radicals; D005659:Fungicides, Industrial / Q000506:poisoning*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008708:Methemoglobinemia / Q000139:chemically induced* / Q000188:drug therapy; D008751:Methylene Blue / Q000627:therapeutic use; D009569:Nitric Oxide / Q000378:metabolism; D010084:Oxidation-Reduction; D010720:Phosphines / Q000506:poisoning*; D051381:Rats; D017382:Reactive Oxygen Species; D019163:Reducing Agents / Q000627:therapeutic use
D017607:Aluminum Compounds; D005609:Free Radicals; D005659:Fungicides, Industrial; D010720:Phosphines; D017382:Reactive Oxygen Species; D019163:Reducing Agents; D009569:Nitric Oxide; D008315:Malondialdehyde; C001864:aluminum phosphide; D008751:Methylene Blue