Determination of idarubicin in human urine by capillary zone electrophoresis with amperometric detection.
A simple, reliable and reproducible method, based on capillary zone electrophoresis with amperometric detection, has been developed for the determination of idarubicin in human urine. A carbon disk electrode was used as working electrode. The optimal conditions of separation and detection were pH 5.6 phosphate buffer (0.20 mol/L), 22 kV for the separation voltage and 1.00 V (vs. Ag/AgCl, 3 mol/L KCl) for the detection potential. The linear range was from 4.0 x 10(-7) to 2.0 x 10(-5) mol/L with a regression coefficient of 0.9986, and the detection limit was 8.0 x 10(-8) mol/L. The method was directly applied to the determination of idarubicin in spiked human urine without any other sample pretreatment except filtration, and the assay results were satisfactory.
Department of Chemistry, East China Normal University, Shang hai. yuzhi@online.sh.cn
Q
Q
Hu
T
T
Zhou
L
L
Zhang
H
H
Li
Y
Y
Fang
D000903:Antibiotics, Antineoplastic / Q000652:urine*; D004563:Electrochemistry; D004566:Electrodes; D019075:Electrophoresis, Capillary; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015255:Idarubicin / Q000652:urine*; D012044:Regression Analysis
D000903:Antibiotics, Antineoplastic; D015255:Idarubicin