[The effect of tissue inhibitor of metalloproteinase-2 gene (TIMP-2 gene) on gastric carcinoma cells in vitro and in vivo].
OBJECTIVE
To study the effect of TIMP-2 gene on the invasive behavior of gastric carcinoma cells and to explore the possibility of blocking tumor metastasis.


METHODS
TIMP-2 gene was transferred into human gastric cancer cell line SGC-7901 by lipofection technology. The ability of tumor cell invasion was detected by Boyden chamber in vitro and tumor invasion animal model in vivo. Type IV collagen and type IV collagenase were detected by immunohistochemical technique.


RESULTS
The invasive ability of gastric cancer cells SGC-7901 transfected with TIMP-2 gene was significantly inhibited in vivo and in vitro. The expression of type IV collagenase in the untransfected group was higher than in the transfected group.


CONCLUSIONS
TIMP-2 gene transfection can reduce the ability of gastric cancer cell invasion in vitro and in vivo.
Li
N
N
Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing 400038.
Xu
C
C
Liu
W
W
D000230:Adenocarcinoma / Q000473:pathology*; D000818:Animals; D003094:Collagen / Q000378:metabolism; D018014:Gene Transfer Techniques; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D013274:Stomach Neoplasms / Q000473:pathology*; D013370:Subrenal Capsule Assay; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000096:biosynthesis / Q000235:genetics*; D014407:Tumor Cells, Cultured
D019716:Tissue Inhibitor of Metalloproteinase-2; D003094:Collagen; D020780:Matrix Metalloproteinase 9