Sensitive measurement of polyethylene glycol-modified proteins.
An IgM monoclonal antibody (AGP3) against polyethylene glycol (PEG) was used to assay PEG-modified proteins by ELISA. PEG-modified beta-glucuronidase could be measured at concentrations as low as 15 ng/mL, corresponding to 750 pg (1.8 fmol) of conjugate. This ELISA should be generally applicable to all PEG-modified proteins because AGP3 binds the backbone of the PEG chain independent of the linker used for PEG attachment.
Tsai
N M
NM
Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan.
Cheng
T L
TL
Roffler
S R
SR
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D015151:Immunoblotting; D007075:Immunoglobulin M / Q000276:immunology; D051379:Mice; D011092:Polyethylene Glycols / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D007075:Immunoglobulin M; D011506:Proteins; D011092:Polyethylene Glycols