A physical map of human chromosome 14.
We report the construction of a tiling path of around 650 clones covering more than 99% of human chromosome 14. Clone overlap information to assemble the map was derived by comparing fully sequenced clones with a database of clone end sequences (sequence tag connector strategy). We selected homogeneously distributed seed points using an auxiliary high-resolution radiation hybrid map comprising 1,895 distinct positions. The high long-range continuity and low redundancy of the tiling path indicates that the sequence tag connector approach compares favourably with alternative mapping strategies.
Genoscope and CNRS UMR 8030, Evry, France.
T
T
Brüls
G
G
Gyapay
J L
JL
Petit
F
F
Artiguenave
V
V
Vico
S
S
Qin
A M
AM
Tin-Wollam
C
C
Da Silva
D
D
Muselet
D
D
Mavel
E
E
Pelletier
M
M
Levy
A
A
Fujiyama
F
F
Matsuda
R
R
Wilson
L
L
Rowen
L
L
Hood
J
J
Weissenbach
W
W
Saurin
R
R
Heilig
D022202:Chromosomes, Artificial, Bacterial; D002883:Chromosomes, Human, Pair 14; D003001:Cloning, Molecular; D004926:Escherichia coli; D006801:Humans; D020161:Physical Chromosome Mapping; D021101:Radiation Hybrid Mapping; D016324:Sequence Tagged Sites