In vivo chromatin remodeling by yeast ISWI homologs Isw1p and Isw2p.
Isw1p and Isw2p are budding yeast homologs of the Drosophila ISWI chromatin-remodeling ATPase. Using indirect-end-label and chromatin immunoprecipitation analysis, we show both independent and cooperative Isw1p- and Isw2p-mediated positioning of short nucleosome arrays in gene-regulatory elements at a variety of transcription units in vivo. We present evidence that both yeast ISWI complexes regulate developmental responses to starvation and that for Isw2p, recruitment by different DNA-binding proteins controls meiosis and haploid invasive growth.
Kent
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NA
Division of Molecular Genetics, Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK. nakent@molbiol.ox.ac.uk
Karabetsou
N
N
Politis
P K
PK
Mellor
J
J
D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000378:metabolism*; D015152:Blotting, Northern; D002843:Chromatin / Q000737:chemistry / Q000378:metabolism*; D018983:DNA Footprinting; D004271:DNA, Fungal / Q000032:analysis; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism*; D008540:Meiosis; D008836:Micrococcal Nuclease; D009693:Nucleic Acid Hybridization; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000254:growth & development; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*
D002843:Chromatin; D004271:DNA, Fungal; D005656:Fungal Proteins; C086468:ISWI protein; D014157:Transcription Factors; D008836:Micrococcal Nuclease; D000251:Adenosine Triphosphatases