A highly sensitive fluorimetric method for the determination of fluoride in biological material with Al3+-calcein complex.
A highly sensitive fluorimetric method for the determination of fluoride was established. The method was based on quenching of the fluorescence of the Al3+-calcein complex in CCl3COOH-CH3COOK buffer solution by fluoride. The fluorescence emission was measured at lambdaex/lambdaem 480/503 nm, and the experimental variables and interference in this determination were studied. The linear calibration range was 0.8 ng/mL to 150 ng/mL and the detection limit was 0.2 ng/mL. The method was applied to determine fluoride in biological materials. The recovery was in the range of 96.3% to 104.7% and the relative standard deviation was 4.6%. The results obtained from the certified reference material by the present method agreed with the certified values.
Department of Botany, The University of Hong Kong, Hong Kong.
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Li
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Chen
D000535:Aluminum / Q000737:chemistry; D000818:Animals; D002138:Calibration; D004785:Environmental Pollutants / Q000032:analysis*; D005452:Fluoresceins / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D005459:Fluorides / Q000032:analysis*; D005470:Fluorometry / Q000379:methods / Q000592:standards; D006801:Humans; D006863:Hydrogen-Ion Concentration; D018515:Plant Leaves / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013696:Temperature
D004785:Environmental Pollutants; D005452:Fluoresceins; D005456:Fluorescent Dyes; D000535:Aluminum; D005459:Fluorides; C007740:fluorexon