Effects of distance and pollen competition on gene flow in the wind-pollinated grass Festuca pratensis Huds.
Pollen dispersal and gene flow in the grass meadow fescue (Festuca pratensis Huds.) were studied using two populations which were homozygous for different allozymes at the Gpi-2 locus. The populations were established in a concentric donor-acceptor field experiment. Gene flow was found mainly to be affected by the distance between the donor and acceptor plants. Analysing 21 132 acceptor plant progenies, gene flow was shown to decrease rapidly with distance to the donor field up to 75 m, and beyond this distance much more slowly. The ability of donor pollen to fertilize acceptor plants depended very much on the density of the acceptor plants. Pairs of acceptor plants produced more compatible pollen locally, and captured significantly less donor pollen than single-plants. Despite the higher seed production of acceptor plants planted in pairs, the absolute number of heterozygous seeds carrying the donor allele was always lower than for single-plants. Wind direction had only a slight effect upon the type of pollen captured. Because of pollen production within the two plant populations being continuous and overlapping, the time when anthesis occurred had little effect on gene flow between the populations. Vigorous and tall acceptor plants with many panicles, high seed yield and high 1000-seed weight were able to capture more donor pollen than shorter plants. The results may be used to assess the risk of gene flow and to develop strategies for monitoring the spread of transgenes from genetically modified grasses.
Rognli
O A
OA
Department of Chemistry and Biotechnology, Agricultural University of Norway, P.O. Box 5040, N-1432 As, Norway. odd-arne.rognli@ikb.nlh.no
Nilsson
N O
NO
Nurminiemi
M
M
D005787:Gene Frequency / Q000235:genetics; D017343:Genes, Plant / Q000235:genetics; D005828:Genetics, Population; D010641:Phenotype; D006109:Poaceae / Q000235:genetics* / Q000502:physiology; D011058:Pollen / Q000235:genetics* / Q000502:physiology; D014919:Wind