Nonresonant MALDI of oligonucleotides: mechanism of ion desorption.
Oligonucleotide ions have been detected using matrix-assisted laser desorption/ionization (MALDI) under nonresonant laser irradiation of the sample. When mass resolution was not limited by adduct attachment to the analyte ions, the nonresonant MALDI spectra demonstrated better resolution than the spectra acquired with resonant ultraviolet irradiation. We found that preparation of thin-film samples on absorbing substrate surfaces was critical for the success of NR-MALDI. The possible acoustic mechanisms of ion formation and desorption are discussed.
Oak Ridge National Laboratory, Tennessee 37831-6378, USA.
V V
VV
Golovlev
S H
SH
Lee
S L
SL
Allman
N I
NI
Taranenko
N R
NR
Isola
C H
CH
Chen
D009841:Oligonucleotides / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013056:Spectrophotometry, Ultraviolet
D009841:Oligonucleotides