Fission yeast Pom1p kinase activity is cell cycle regulated and essential for cellular symmetry during growth and division.
Schizosaccharomyces pombe cells grow from both ends during most of interphase and divide symmetrically into two daughter cells. The pom1 gene, encoding a member of the Dyrk family of protein kinases, has been identified through a mutant showing abnormal cellular morphogenesis. Here we show that Pom1p kinase activity is cell cycle regulated in correlation with the state of cellular symmetry: the activity is high during symmetrical growth and division, but lower when cells grow at just one end. Point mutations in the catalytic domain lead to asymmetry during both cell growth and division, whilst cells overexpressing Pom1p form additional growing ends. Manipulations of kinase activity indicate a negative role for Pom1p in microtubule growth at cell ends. Pom1p is present in a large protein complex and requires its non-catalytic domain to localize to the cell periphery and its kinase activity to localize to cell ends. These data establish that Pom1p kinase activity plays an important role in generating cellular symmetry and suggest that there may be related roles of homologous protein kinases ubiquitously present in all eukaryotes.
Bähler
J
J
Imperial Cancer Research Fund, Cell Cycle Laboratory, 44 Lincoln's Inn Fields, London WC2A 3PX, UK. jurg@sanger.ac.uk
Nurse
P
P
D015153:Blotting, Western; D020134:Catalytic Domain; D002453:Cell Cycle; D002455:Cell Division; D003599:Cytoskeleton / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D046911:Macromolecular Substances; D009154:Mutation; D011233:Precipitin Tests; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport; D011993:Recombinant Fusion Proteins; D012568:Schizosaccharomyces / Q000166:cytology* / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D029702:Schizosaccharomyces pombe Proteins
D046911:Macromolecular Substances; D011993:Recombinant Fusion Proteins; D029702:Schizosaccharomyces pombe Proteins; D011494:Protein Kinases; C112212:Pom1 protein, S pombe