[Primed in situ labelling of human metaphase chromosomes].
Primed in situ labelling (PRINS) contributes to the elucidation of the organization of eukaryotic chromosomes and provides an alternative way to localize gene on chromosomes. Here we present the results of random primer and SOX degenerate primer PRINS on human metaphase chromosomes. The particular band pattern of dark and light was observed by using random primer PRINS. SOX degenerate primer PRINS revealed more SOX gene loci and proved further that SOX genes were not clustered on human chromosomes.
College of Life Sciences, Wuhan University, Wuhan 430072, China.
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YQ
Guo
H H
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Cheng
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Huang
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RJ
Zhou
D002874:Chromosome Mapping; D004268:DNA-Binding Proteins / Q000235:genetics; D006609:High Mobility Group Proteins / Q000235:genetics*; D006801:Humans; D008677:Metaphase; D009419:Nerve Tissue Proteins / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics; D055748:SOXB1 Transcription Factors; D055751:SOXC Transcription Factors; D055755:SOXD Transcription Factors; D015534:Trans-Activators / Q000235:genetics; D014157:Transcription Factors
D004268:DNA-Binding Proteins; D006609:High Mobility Group Proteins; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; C490906:SOX1 protein, human; C107423:SOX12 protein, human; C083431:SOX4 protein, human; C490301:SOX5 protein, human; D055748:SOXB1 Transcription Factors; D055751:SOXC Transcription Factors; D055755:SOXD Transcription Factors; C489990:Sox4 protein, mouse; D015534:Trans-Activators; D014157:Transcription Factors