Mild postischemic hypothermia is neuroprotective in the immature rat neocortex slice.
Mild hypothermia as an intervention after perinatal asphyxia may prevent neurological damage in the newborn. We used stimulus-induced field potentials to monitor recovery from oxygen and glucose deprivation (OGD) in neocortex slices of 6-8-day-old wistar rats. OGD after a latency of 10.7+/-2.1 min (mean+/-S.E.) resulted in an anoxic depolarisation with an amplitude of 5.4+/-2.4 mV. Mild hypothermia of 31 degrees C (vs. 35 degrees C in the control group) was applied for 60 min after end of OGD. The 20, 40, 60 and 80% recovery of the field potential amplitude was significantly faster in the hypothermia group in comparison to the control group. These data indicate that mild postischemic hypothermia may have neuroprotective effects after perinatal asphyxia.
Department of Neonatology, Charité Campus Virchow-Klinikum, Augustenburger Platz 1, D-13353, Berlin, Germany. hae-kyung.ko@rz.hu-berlin.de
H
H
Ko
M
M
Zeller
S
S
Gabriel
J
J
Graulich
U
U
Heinemann
M
M
Obladen
D000818:Animals; D000831:Animals, Newborn; D001831:Body Temperature; D002545:Brain Ischemia / Q000503:physiopathology* / Q000628:therapy*; D005260:Female; D005947:Glucose / Q000494:pharmacology; D007036:Hypothermia, Induced; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D019579:Neocortex / Q000503:physiopathology*; D009924:Organ Culture Techniques; D010100:Oxygen / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D005947:Glucose; D010100:Oxygen