Restoration of fertility in young obese (Lep(ob) Lep(ob)) male mice with low dose recombinant mouse leptin treatment.
OBJECTIVE
We investigated the effects of low-dose leptin treatment on restoration of fertility in young adult male leptin deficient obese mice.


RESULTS
MMTV-TGF-alpha Lep(ob) Lep(ob) mice (8--10 weeks old) were treated with recombinant mouse leptin. In experiment 1, four mice (5 microg/g body weight leptin followed by 2.5 microg/g) lost weight and impregnated females (number of pregnancies/number of females, 3/6, 5/6, 5/10, 4/10). In experiment 2, Leptin-Obese (2.5 microg/g) and Control-Lean mice weighed significantly less than Control-Obese mice. Epididymal pad weights of Control-Obese mice were the heaviest, followed by those of Leptin-Obese mice, and Control-Lean mice were the lightest. Testes weight was greater in Control-Lean vs Control-Obese mice. Leptin-Obese mice had testes weight not significantly different from either control group. Four of five Leptin-Obese mice impregnated females (4/10, 5/10, 2/10, 5/12, 0/10).


CONCLUSIONS
These results indicate that low-dosage mouse recombinant leptin treatment restored fertility to young Lep(ob) Lep(ob) male mice. Although body weights of Leptin-Obese mice were similar to those of lean age-matched mice, epididymal fat pad weights were heavier. International Journal of Obesity (2001) 25, 95-97
Cleary
M P
MP
The Hormel Institute, University of Minnesota, Austin, Minnesota 55912, USA. mpcleary@smig.net
Bergstrom
H M
HM
Dodge
T L
TL
Getzin
S C
SC
Jacobson
M K
MK
Phillips
F C
FC
D000818:Animals; D005260:Female; D005298:Fertility / Q000187:drug effects*; D020738:Leptin / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D009765:Obesity / Q000188:drug therapy* / Q000503:physiopathology; D009929:Organ Size; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D015431:Weight Loss / Q000187:drug effects*
D020738:Leptin; D011994:Recombinant Proteins