Quantification and in situ localization of MCP-1 mRNA and its relation to the immune response of renal cell carcinoma.
Malignant tumours are usually accompanied by an immune response. Chemokines such as MCP-1 have been claimed to be potent inducers of such tumour-associated reactions. In the present study MCP-1 mRNA was quantified by competitive reverse transcription polymerase reaction and localised by in situ hybridisation in renal cell carcinoma tissue in comparison to tumour-free tissue of the same nephrectomy specimen. MCP-1 mRNA levels were correlated with the immune cell infiltrate, the density of CD31(+)microvessels, and the endothelial expression of ICAM-1, VCAM-1, E-, and P-selectin. In only seven of 19 cases, MCP-1 mRNA levels in carcinoma tissue were increased in comparison to tumour-free tissue. Within tumour tissue, mRNA transcripts could be localised in tumour cells, microvessel endothelia, and in tumour-associated macrophages. A correlation between MCP-1 mRNA levels and the density of immune cells, especially macrophages, the microvessel density, and the expression of adhesion molecules could not be observed. Therefore, MCP-1 seems to be of minor importance for the induction of an immune response in renal cell carcinomas regarding at least the parameters analysed in this study.
Hemmerlein
B
B
Department of Pathology, University of Göttingen, Robert-Koch-Str. 40, D-37075 Göttingen, Germany. hemmer@med.uni-goettingen.de
Johanns
U
U
Kugler
A
A
Reffelmann
M
M
Radzun
H J
HJ
D002292:Carcinoma, Renal Cell / Q000098:blood supply / Q000737:chemistry / Q000276:immunology* / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000096:biosynthesis; D018932:Chemokine CCL2 / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D017403:In Situ Hybridization; D007668:Kidney / Q000098:blood supply / Q000737:chemistry / Q000276:immunology / Q000473:pathology; D007680:Kidney Neoplasms / Q000098:blood supply / Q000737:chemistry / Q000276:immunology* / Q000473:pathology; D008833:Microcirculation / Q000276:immunology / Q000473:pathology; D020556:Neutrophil Infiltration / Q000276:immunology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism*
D015815:Cell Adhesion Molecules; D018932:Chemokine CCL2; D012333:RNA, Messenger