Expression of the Hoxa-13 gene correlates to hepatitis B and C virus associated HCC.
To study the Hoxa-13 gene in the liver, we examined its expression by RT-PCR in various liver cell lines, rat livers under different conditions, and human primary hepatocellular carcinomas (HCCs). The gene was found to be expressed in cell lines originating from liver stem-like cells, but not in cell lines originating from hepatocytes and bile duct epithelia. Expression was induced in rat livers after treatment with d-galactosamine, which is known to induce oval cell proliferation, but not after a two-thirds partial hepatectomy (2/3 PH) where induction of oval cell proliferation is thought not to occur. Expression of the gene correlated with human HCC samples associated with Hepatitis B or C virus infection in this small series. These results suggest that the Hoxa-13 gene may provide a potentially useful tool for elucidation of mechanisms involved in lineage-specific differentiation and carcinogenesis of liver stem cells.
Department of Molecular Biology, Beckman Research Institute of City of Hope, 1450 East Duarte Road, Duarte, California 91010, USA.
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T
Huang
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Chesnokov
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Yokoyama
B I
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Carr
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Itakura
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology / Q000821:virology; D002460:Cell Line; D005688:Galactosamine / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic; D016174:Hepacivirus; D006498:Hepatectomy; D006515:Hepatitis B virus; D006525:Hepatitis, Viral, Human / Q000235:genetics / Q000821:virology; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology / Q000821:virology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D018398:Homeodomain Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; C099959:homeobox protein HOXA13; D005688:Galactosamine