Expression of p75NTR and its associated protein NADE in the rat cochlea.
OBJECTIVE
To investigate the expression of the low-affinity neurotrophin receptor p75 (p75NTR) and its associated protein NADE in the cochlea of the developing and the adult rat. Studies such as this one will help to predict the functional role of p75NTR and NADE in cochlear development.


METHODS
Histochemical evaluation of p75NTR and NADE in the rat cochlea was performed.


METHODS
Immunohistochemical analysis was used to localize p75NTR and NADE in the rat cochlea at postnatal (PN) days PN0, PN2, PN4, PN6, PN8, PN10, and PN13 and in the adult. Confocal laser scanning microscopy was used to analyze whole-mount specimens.


RESULTS
Immunoreactivity of both p75NTR and NADE was observed in pillar cells. However, these proteins displayed reciprocal expression patterns. Expression of p75NTR was detected at PN0 and PN2, but disappeared after PN4. In contrast, NADE expression was initially detected at PN2 and persisted into adulthood.


CONCLUSIONS
The neurotrophin receptor p75NTR and NADE have distinct and independent roles in developing and mature cochlea.
Department of Otolaryngology, School of Medicine, Kitasato University, Sagamihara, Japan.
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Sano
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Mukai
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Monoo
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D000367:Age Factors; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D051017:Apoptosis Regulatory Proteins; D003051:Cochlea / Q000033:anatomy & histology*; D007760:Labyrinth Supporting Cells / Q000648:ultrastructure; D009414:Nerve Growth Factors / Q000032:analysis; D009925:Organ of Corti / Q000033:anatomy & histology; D011506:Proteins / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D020800:Receptor, Nerve Growth Factor; D017475:Receptors, Nerve Growth Factor / Q000032:analysis*; D012016:Reference Values
D051017:Apoptosis Regulatory Proteins; C485843:Bex3 protein, rat; D009414:Nerve Growth Factors; D011506:Proteins; D020800:Receptor, Nerve Growth Factor; D017475:Receptors, Nerve Growth Factor