Studies on cytotoxicity of nickel ions using C3H10T1/2 fibroblast cells.
Cytotoxicity of Ni ions were examined using C3H mouse derived 10T1/2 fibroblast cells. It became evident that Ni ions had dose-dependent cytotoxicity. The cell number incubated in DME medium containing 0.04 mM/L Ni ion for 6 days was reduced to half that in control DME medium without Ni. The cell totally disappeared in DME medium containing 2 mM/L Ni ion. The dissolution of Ni ions from Ni-containing metallic restorations must be lower than these concentration levels so that oral tissues might not be damaged. No neoplastic transformation was found on all cells examined.
Department of Dental Materials Science and Technology, Osaka University Faculty of Dentistry, Osaka, Japan. mtaira@iwate-med.ac.jp
M
M
Taira
M S
MS
Toguchi
Y
Y
Hamada
M
M
Okazaki
J
J
Takahashi
R
R
Ito
S
S
Toyosawa
N
N
Ijyuin
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects*; D007477:Ions; D007928:Lethal Dose 50; D051379:Mice; D008809:Mice, Inbred C3H; D009532:Nickel / Q000633:toxicity*; D017942:Nitric Acid / Q000737:chemistry; D017693:Sodium Bicarbonate / Q000737:chemistry
D003470:Culture Media; D007477:Ions; D017942:Nitric Acid; D009532:Nickel; D017693:Sodium Bicarbonate