Functional cooperation between topoisomerase I and single strand DNA-binding protein.
Protein-protein interactions play important role in cell biochemistry by favorably or adversely influencing major molecular events. In most documented cases, the interaction is direct between the partner molecules. Influence of activity in the absence of direct physical interaction between DNA transaction proteins is another important means of modulation. We show here that single strand binding protein stimulates DNA topoisomerase I activity without direct protein-protein interactions. The stimulation is specific to topoisomerase I, as DNA gyrase activity is unaffected by SSB. We propose that such cases of functional collaboration between DNA transaction proteins play important roles in vivo.
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, 560012, India.
D
D
Sikder
S
S
Unniraman
T
T
Bhaduri
V
V
Nagaraja
D019921:Biotinylation; D002850:Chromatography, Gel; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004264:DNA Topoisomerases, Type I / Q000378:metabolism*; D004250:DNA Topoisomerases, Type II / Q000378:metabolism; D004278:DNA, Superhelical / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004789:Enzyme Activation; D004926:Escherichia coli / Q000737:chemistry / Q000201:enzymology; D005976:Glutaral / Q000378:metabolism; D007700:Kinetics; D009161:Mycobacterium / Q000201:enzymology*; D009690:Nucleic Acid Conformation; D011233:Precipitin Tests; D011485:Protein Binding; D013379:Substrate Specificity; D020349:Surface Plasmon Resonance; D013816:Thermodynamics
D004278:DNA, Superhelical; D004268:DNA-Binding Proteins; D004247:DNA; D004264:DNA Topoisomerases, Type I; D004250:DNA Topoisomerases, Type II; D005976:Glutaral