Requirement of a centrosomal activity for cell cycle progression through G1 into S phase.
Centrosomes were microsurgically removed from BSC-1 African green monkey kidney cells before the completion of S phase. Karyoplasts (acentrosomal cells) entered and completed mitosis. However, postmitotic karyoplasts arrested before S phase, whereas adjacent control cells divided repeatedly. Postmitotic karyoplasts assembled a microtubule-organizing center containing gamma-tubulin and pericentrin, but did not regenerate centrioles. These observations reveal the existence of an activity associated with core centrosomal structures-distinct from elements of the microtubule-organizing center-that is required for the somatic cell cycle to progress through G1 into S phase. Once the cell is in S phase, these core structures are not needed for the G2-M phase transition.
Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01605, USA., Laboratory of Cell Regulation, Wadsworth Center, Albany, NY 12201, USA.
E H
EH
Hinchcliffe
F J
FJ
Miller
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M
Cham
A
A
Khodjakov
G
G
Sluder
D000818:Animals; D000941:Antigens / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002502:Centrioles / Q000502:physiology; D018385:Centrosome / Q000502:physiology*; D002522:Chlorocebus aethiops; D003594:Cytoplasmic Granules / Q000502:physiology / Q000648:ultrastructure; D016193:G1 Phase; D007399:Interphase; D018715:Microscopy, Video; D022101:Microtubule-Organizing Center / Q000502:physiology; D008870:Microtubules / Q000502:physiology / Q000648:ultrastructure; D008938:Mitosis; D017239:Paclitaxel / Q000494:pharmacology; D016196:S Phase; D008941:Spindle Apparatus / Q000502:physiology / Q000648:ultrastructure; D014404:Tubulin / Q000378:metabolism
D000941:Antigens; D014404:Tubulin; C086178:pericentrin; D017239:Paclitaxel