Effects of pH variation and NaCl on in vitro digestibility of cow's milk proteins in commercially available infant formulas.
Allergenicity and antigenicity of food proteins are generally dependent on their heat-stability and resistance to digestive enzymes. Using the methods of SDS-PAGE and immunoblots, we assessed peptic-digestibility of major milk proteins (casein, beta-lactoglobulin and alpha-lactalbumin) in commercially available infant formulas in acidic pH range (pH 1.5 to 4.0), and we also investigated the effect of NaCl on peptic-digestibility of cow's milk proteins at pH 2.0. The profile of peptic-digestion of casein was similar at pH range from 1.5 to 3.5 where the intact protein of casein rapidly disappeared, whereas slow digestibility of the protein was found at pH 4.0. Beta-lactoglobulin and alpha-lactalbumin were digested at pH range from 1.5 to 2.5 and were almost entirely resistant to peptic digestion at over pH 3.0. In the presence of NaCl at pH 2.0, casein showed a good digestibility by pepsin similar to that in the absence of NaCl, in contrast to beta-lactoglobulin and alpha-lactalbumin both having their peptic-digestibility decreased in the presence of 0.2 M NaCl. As the state of the stomach in new born infants shows low amounts of secretary pepsin and out of optimum pH of peptic activity, low digestibility of beta-lactoglobulin and alpha-lactalbumin in cow's milk based infant formulas at over pH 3.0 is supposed to be responsible for their allergenicity.
Sakai
K
K
Department of Nutrition, School of Medicine, The University of Tokushima, Japan. sakai@nutr.med.tokushima-u.ac.jp
Yoshino
K
K
Satter
M A
MA
Ota
F
F
Nii
Y
Y
Fukuta
K
K
Ueda
N
N
Shimizu
Y
Y
Yamamoto
S
S
D000818:Animals; D002417:Cattle; D004063:Digestion; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015151:Immunoblotting; D007225:Infant Food; D007231:Infant, Newborn; D007768:Lactalbumin / Q000276:immunology / Q000378:metabolism; D007782:Lactoglobulins / Q000276:immunology / Q000378:metabolism; D008892:Milk / Q000378:metabolism; D008894:Milk Proteins / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D010434:Pepsin A / Q000378:metabolism; D012965:Sodium Chloride / Q000737:chemistry
D007782:Lactoglobulins; D008894:Milk Proteins; D012965:Sodium Chloride; D007768:Lactalbumin; D010434:Pepsin A