Expression and characterization of a magnetosome-associated protein, TPR-containing MAM22, in Escherichia coli.
A magnetosome-associated protein, MAM22, contains a TPR domain (five TPR motifs and one putative TPR motif) that has been known to mediate protein-protein interactions. We expressed the mam22 gene in Escherichia coli and found that the purified MAM22 was reversibly self-aggregated by NaCl. The structural model of MAM22 which has been proposed on the basis of the crystal structure of the N-terminal TPR domain of a human Ser/Thr protein phosphatase suggests the novel hydrophobic colloidal features of MAM22 with TPR motifs.
Y
Y
Okuda
Y
Y
Fukumori
D020816:Amino Acid Motifs / Q000235:genetics*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D008280:Magnetics; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D008958:Models, Molecular; D008969:Molecular Sequence Data; D028861:Nuclear Pore Complex Proteins; D011485:Protein Binding / Q000187:drug effects; D011487:Protein Conformation; D011518:Proto-Oncogene Proteins / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D012244:Rhodospirillaceae / Q000235:genetics*; D020539:Sequence Analysis, Protein; D012965:Sodium Chloride / Q000494:pharmacology; D014170:Transformation, Genetic
D001426:Bacterial Proteins; C099987:MAM22 protein, Magnetospirillum magnetotacticum; D008565:Membrane Proteins; D028861:Nuclear Pore Complex Proteins; D011518:Proto-Oncogene Proteins; D011993:Recombinant Fusion Proteins; C073597:TPR protein, human; D012965:Sodium Chloride