Use of PFGE typing for tracing contamination with Listeria monocytogenes in three cold-smoked salmon processing plants.
The sites of Listeria monocytogenes contamination in three cold-smoked salmon (Salmo salar) processing plants were detected by sampling salmon and the plant's environment and equipment at different production stages. Of the 141 samples collected from three processing plants, 59 (42%) were contaminated with L. monocytogenes. The rates of contamination varied as to the plant and the sample source. L. monocytogenes isolates from 17 various contaminated seafood products (fresh, frozen and smoked fishes, cooked mussels) were also studied. A total of 155 isolates from the three plants and the various seafoods were characterized by genomic macrorestriction using ApaI and SmaI with pulsed-field gel electrophoresis (PFGE) and 82 isolates were serotyped. Macrorestriction yielded 20 pulsotypes and serotyping yielded four serovars: 1/2a, 1/2b, 1/2c, 4b (or e), with 77 (93%) belonging to serovar 1/2a. One clone of L. monocvtogenes predominated and persisted in plant I and was the only pulsotype detected in the final product although it was not isolated from raw salmon. No L. monocytogenes was detected in the smoked skinned salmon processed in plant II, even though 87% of the raw salmon was contaminated. All the smoked salmon samples collected in plant III were contaminated with a unique clone of L. monocytogenes, which may have occurred during slicing. In the three plants, the contamination of final products did not seem to originate from the L. monocytogenes present on raw salmon, but from the processing environment.
Agence Française de Sécurité Sanitaire des Aliments, Laboratoire d'Etude des Produits de la Mer, Boulogne sur Mer, France. g.dauphin@afssa.fr
G
G
Dauphin
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Ragimbeau
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P
Malle
D000818:Animals; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D005506:Food Contamination; D005511:Food Handling; D005525:Food-Processing Industry; D008089:Listeria monocytogenes / Q000235:genetics / Q000302:isolation & purification*; D012474:Salmon / Q000382:microbiology*; D012703:Serotyping
D004269:DNA, Bacterial