New insight into RNase P RNA structure from comparative analysis of the archaeal RNA.
A detailed comparative analysis of archaeal RNase P RNA structure and a comparison of the resulting structural information with that of the bacterial RNA reveals that the archaeal RNase P RNAs are strikingly similar to those of Bacteria. The differences between the secondary structure models of archaeal and bacterial RNase P RNA have largely disappeared, and even variation in the sequence and structure of the RNAs are similar in extent and type. The structure of the cruciform (P7-11) has been reevaluated on the basis of a total of 321 bacterial and archaeal sequences, leading to a model for the structure of this region of the RNA that includes an extension to P11 that consistently organizes the cruciform and adjacent highly-conserved sequences.
Department of Microbiology, North Carolina State University, Raleigh 27695, USA.
J K
JK
Harris
E S
ES
Haas
D
D
Williams
D N
DN
Frank
J W
JW
Brown
D001483:Base Sequence; D002478:Cells, Cultured; D017931:DNA Primers / Q000737:chemistry; D004722:Endoribonucleases / Q000737:chemistry*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D010957:Plasmids; D016133:Polymerase Chain Reaction; D019642:RNA, Archaeal / Q000145:classification / Q000235:genetics*; D012329:RNA, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D016337:RNA, Catalytic / Q000737:chemistry* / Q000302:isolation & purification; D043262:Ribonuclease P; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D029968:Escherichia coli Proteins; D019642:RNA, Archaeal; D012329:RNA, Bacterial; D016337:RNA, Catalytic; D004722:Endoribonucleases; D043262:Ribonuclease P; C470865:ribonuclease P, E coli