Effects of ouabain on the growth and DNA synthesis of PC12 cells.
We investigated the effects of ouabain and serum from salt-loaded Dahl salt-sensitive (S) rats, which contain abundant ouabain-like compounds, on the growth and DNA synthesis of rat pheochromocytoma PC12 cells. Ouabain decreased the growth of PC12 cells, as evaluated by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay, in a concentration-dependent fashion. A moderate concentration (10(-7) M) of ouabain increased DNA synthesis, as measured by 5-bromo-2'-deoxyuridine incorporation, and induced transcription of the proto-oncogenes c-myc and c-fos. Serum from salt-loaded Dahl S rats also enhanced DNA synthesis, but serum from Dahl salt-resistant rats did not. Thus ouabain-like compounds may modify the growth or differentiation of neural tissues. This effect may contribute to the development of salt-induced hypertension in Dahl S rats.
Department of Internal Medicine, University of Tokyo, Japan. katsuatky@umin.ac.jp
K
K
Ando
N
N
Omi
T
T
Shimosawa
K
K
Takahashi
T
T
Fujita
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D016259:Genes, myc; D006973:Hypertension / Q000209:etiology*; D020932:Nerve Growth Factor / Q000096:biosynthesis; D010042:Ouabain / Q000494:pharmacology*; D016716:PC12 Cells; D051381:Rats; D020303:Rats, Inbred Dahl; D017673:Sodium Chloride, Dietary / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*
D004791:Enzyme Inhibitors; D017673:Sodium Chloride, Dietary; D010042:Ouabain; D004247:DNA; D020932:Nerve Growth Factor; D000254:Sodium-Potassium-Exchanging ATPase