Laminin-5 promotes neurite outgrowth from central and peripheral chick embryonic neurons.
Laminin-5 (Ln-5) is an essential component of epithelial basal laminae that is also expressed in the developing nervous system. Here we use a convenient, simple and reproducible in vitro fluorescent assay to assess the neurite outgrowth promoting activity of purified Ln-5. Embryonic chick neurons from dorsal root ganglia, ciliary ganglia, and (to a lesser extent) retina extended neurites on Ln-5, but the neurite outgrowth promoting activity was not as great as that of Ln-1 or Ln-2. Neurons from diencephalon, telencephalon, and spinal cord did not respond to Ln-5.
Neuroscience Research Institute and Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, CA 93106, USA.
B
B
Culley
J
J
Murphy
J
J
Babaie
D
D
Nguyen
A
A
Pagel
P
P
Rousselle
D O
DO
Clegg
D000818:Animals; D001921:Brain / Q000166:cytology; D015815:Cell Adhesion Molecules / Q000494:pharmacology*; D002478:Cells, Cultured; D002642:Chick Embryo; D005109:Extracellular Matrix / Q000502:physiology; D005726:Ganglia, Parasympathetic / Q000166:cytology; D005727:Ganglia, Spinal / Q000166:cytology; D008856:Microscopy, Fluorescence / Q000379:methods; D016501:Neurites / Q000187:drug effects* / Q000502:physiology*; D012160:Retina / Q000166:cytology; D013116:Spinal Cord / Q000166:cytology; D000097794:Kalinin
D015815:Cell Adhesion Molecules; D000097794:Kalinin