[An analysis of the clinical and laboratory data of 53 cases of non-Hodgkin's lymphoma].
OBJECTIVE
To analyse the influence of factors on the prognoses of non-Hodgkin's lymphoma (NHL) and the clinical usage of IgH and T cell receptor(TCR) gene rearrangement for NHL typing.


METHODS
Immunological phenotyping was carried out by SABC, and IgH(FR2A, FR3A) and TCR(beta, gamma) detection by PCR.


RESULTS
The age curve of NHL was increased parallel with the patient's age increasing, hazard ratio was increased 1.039n annually. The incidence of B cell NHL(B-NHL) was 66.7%, T cell NHL(T-NHL) was 31.1%, low grade NHL was 56%, middle and high grade NHL were 44%. The positivity of IgH and TCR gene rearrangement in NHL patients were 75% by PCR detection, T and B classification was same as phenotyping. The 3 and 5 years survival ratio: HD were 83.3% and 62.5%, stage I-II of NHL were 88.9% and 66.7%, stage III-IV of NHL were 39.9% and 33.3%, Low grade NHL were 65.1% and 48.8%, middle and high grade NHL were 47.6% and 39.6%. The survival time of APBSCT group was longer than that of the conventional therapy group.


CONCLUSIONS
Age, T,B classification, grading and staging are the important factors which affect on NHL prognoses. APBCST can improve NHL prognoses, especially for those of stage III-IV patients. Molecular biological methods can help T/B classification when it couldn't be confirmed by phenotyping.
Third Hospital, Beijing Medical University, Beijing 100083, China.
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X
Ke
Y
Y
Yang
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Z
Gao
D000328:Adult; D000367:Age Factors; D000368:Aged; D005260:Female; D015329:Gene Rearrangement, T-Lymphocyte / Q000235:genetics; D006801:Humans; D008228:Lymphoma, Non-Hodgkin / Q000175:diagnosis* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D011379:Prognosis; D016019:Survival Analysis