Optical mapping of activation patterns in an animal model of congenital heart block.
Congenital heart block (CHB) is associated with high mortality and affects children of mothers with autoantibodies (IgG) to ribonucleoproteins SSB/La and SSA/Ro. IgG from mothers of children with CHB (positive IgG) was used to assess activation patterns in both the right atrium (RA) and right ventricle (RV) of Langendorff-perfused young rabbit hearts. Optical action potentials (AP) were obtained by using a 124-site photodiode array with 4-[-[2-(di-n-butylamino)-6-naphthyl]vinyl]pyridinium. Optical APs were recorded to simultaneously image activation patterns from the RA and RV. Perfusion of positive IgG (800--1,200 micro resulted in sinus bradycardia and varying degrees of heart block. Activation maps revealed marked conduction delay at the sinoatrial junction but only minor changes in overall atrial and ventricular activation patterns. No conduction disturbances were seen in the presence of IgG from mothers with healthy children. In conclusion, besides atrioventricular (AV) block, positive IgG induces sinus bradycardia. These results establish that the sequelae of CHB are associated with impaired intrasinus and/or sinoatrial conduction. The findings raise the possibility that sinus bradycardia in the developing heart may indicate the potential for AV conduction disturbances.
Restivo
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Molecular and Cellular Cardiology Program, Veterans Affairs New York Harbor Health Care System, Brooklyn, New York 11209, USA. mrestivo@bigfoot.com
Kozhevnikov
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Boutjdir
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D000200:Action Potentials; D000818:Animals; D016280:Atrial Function, Right / Q000502:physiology*; D001323:Autoantibodies / Q000097:blood; D001919:Bradycardia / Q000276:immunology / Q000503:physiopathology; D004195:Disease Models, Animal; D004562:Electrocardiography / Q000187:drug effects; D005260:Female; D006321:Heart / Q000502:physiology / Q000503:physiopathology*; D006327:Heart Block / Q000151:congenital / Q000276:immunology / Q000503:physiopathology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D010477:Perfusion; D011247:Pregnancy; D011817:Rabbits; D012016:Reference Values; D012261:Ribonucleoproteins / Q000276:immunology; D012849:Sinoatrial Node / Q000503:physiopathology; D016278:Ventricular Function, Right / Q000502:physiology*
D001323:Autoantibodies; D007074:Immunoglobulin G; D012261:Ribonucleoproteins