Duration of sample storage dramatically alters expression of the human immunodeficiency virus coreceptors CXCR4 and CCR5.
Expression of the chemokine receptors CXCR4 and CCR5 was monitored using EDTA-anticoagulated whole blood held for different time periods prior to fluorescent-antibody staining. When left overnight CXCR4 expression on leukocytes was substantially increased, whereas CCR5 expression was reduced. The results were similar when heparin and acid-citrate-dextrose were used as anticoagulants.
Shalekoff
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AIDS Virus Research Unit, National Institute for Virology, and Department of Virology, University of the Witwatersrand, Johannesburg, South Africa.
Tiemessen
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CT
D000163:Acquired Immunodeficiency Syndrome / Q000175:diagnosis / Q000276:immunology*; D000925:Anticoagulants; D015496:CD4-Positive T-Lymphocytes / Q000737:chemistry / Q000378:metabolism* / Q000821:virology; D018414:CD8-Positive T-Lymphocytes / Q000737:chemistry / Q000378:metabolism / Q000821:virology; D015331:Cohort Studies; D004492:Edetic Acid; D005434:Flow Cytometry / Q000592:standards; D006801:Humans; D008137:Longitudinal Studies; D019713:Receptors, CCR5 / Q000032:analysis / Q000378:metabolism*; D019718:Receptors, CXCR4 / Q000032:analysis / Q000378:metabolism*; D013048:Specimen Handling / Q000592:standards*; D013997:Time Factors; D015854:Up-Regulation / Q000276:immunology
D000925:Anticoagulants; D019713:Receptors, CCR5; D019718:Receptors, CXCR4; D004492:Edetic Acid