Rapid typing of Borrelia burgdorferi sensu lato species in specimens from patients with different manifestations of Lyme borreliosis.
To further investigate the pathogenic potential of different Borrelia burgdorferi genospecies, specimens from 27 patients with different manifestations of Lyme borreliosis were analyzed by PCR and reverse line blotting (RLB). In samples from Lyme arthritis patients, B. burgdorferi sensu stricto was predominantly identified, while in patients with neuroborreliosis or acrodermatitis, Borrelia garinii and Borrelia afzelii, respectively, were exclusively detected. The results demonstrate that PCR-RLB is a valuable tool for epidemiological and pathogenetic studies of Lyme borreliosis.
Department of Medicine, Rheumatology and Clinical Immunology, Charité University Hospital, 10098 Berlin, Germany.
J D
JD
Lünemann
S
S
Zarmas
S
S
Priem
J
J
Franz
R
R
Zschenderlein
E
E
Aberer
R
R
Klein
L
L
Schouls
G R
GR
Burmester
A
A
Krause
D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000097:blood; D015153:Blotting, Western; D015748:Borrelia burgdorferi Group / Q000145:classification* / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D002648:Child; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D006801:Humans; D008193:Lyme Disease / Q000382:microbiology* / Q000503:physiopathology*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D012867:Skin / Q000382:microbiology; D013582:Synovial Fluid / Q000382:microbiology; D014556:Urine / Q000382:microbiology
D000907:Antibodies, Bacterial; D004269:DNA, Bacterial