[Modulation of epidermal growth factor on the synthesis of pulmonary surfactant and its mechanism].
OBJECTIVE
To study the effect of EGF at physiological level on PS synthesis on cultured lung explants without serum.


METHODS
The total phospholipids and major phospholipid components in lung tissues were determined. The amount of 3H-choline incorporation into PC was measured.


RESULTS
1. 10(-9) mol/L EGF enhanced 3H-choline incorporation into PC at 8 h and reached the maximum at 16 h. 2. The synthesis of total phospholipids, PC and PG increased from lung tissues exposed to 10(-9) mol/L EGF, but membrane characteristic phospholipid showed no change. 3. TP 47, a PTK inhibitor and H7, a PKC inhibitor could block EGF-induced PS synthesis. The release of LDH from lung explants could not be changed by EGF, TP 47 and H7.


CONCLUSIONS
EGF at physiological level could enhance the adult rat PS synthesis, and its modulated mechanisms involved PTK and PKC pathways.
Li
J H
JH
Department of Physiology, Guangzhou Medical College, Guangzhou 510182.
Sun
X H
XH
Luo
Z Q
ZQ
D000818:Animals; D004815:Epidermal Growth Factor / Q000008:administration & dosage / Q000494:pharmacology*; D066298:In Vitro Techniques; D008168:Lung / Q000187:drug effects* / Q000378:metabolism*; D011663:Pulmonary Surfactants / Q000096:biosynthesis*; D051381:Rats
D011663:Pulmonary Surfactants; D004815:Epidermal Growth Factor