Development of the insulin-like growth factor-I assay system.
A high speed full automatic ELISA system for measurement of insulin-like growth factor-I (IGF-I) was established by using magnetic particle-linked monoclonal antibody and enzyme-labeled monoclonal antibody. A standard curve was obtained, and the effect of dilution on the assay system was investigated. An IGF-I spike recovery test of human serum samples and a study of the correlation with a radioimmunoassay system were performed, and good results were obtained from all studies. The assay range was 0.5-50 ng/ml, and the time required for the full automatic measurement was 15 minutes. This assay system will play a central role in the clinical approach to IGF-I.
Osaka City University Medical School, Japan.
T
T
Mine
I
I
Yano
N
N
Tatsumi
Y
Y
Terano
K
K
Yamamoto
T
T
Goda
Y
Y
Kosaka
K
K
Hishida
D000911:Antibodies, Monoclonal; D001331:Automation; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006735:Horseradish Peroxidase; D006801:Humans; D007158:Immunologic Techniques; D007334:Insulin-Like Growth Factor I / Q000032:analysis*; D011994:Recombinant Proteins / Q000032:analysis
D000911:Antibodies, Monoclonal; D011994:Recombinant Proteins; D007334:Insulin-Like Growth Factor I; D006735:Horseradish Peroxidase