Inhibition of 15-lipoxygenase leads to delayed organelle degradation in the reticulocyte.
Mammalian cells are characterized by an endomembrane system. Nevertheless, some cells lose these membranes during their terminal differentiation, e.g. red blood cells and lens fiber cells of the eye. 15-Lipoxygenase is believed to be critical for this membrane degradation. Here we use cultivated rabbit reticulocytes in the presence or absence of a lipoxygenase inhibitor to provide further evidence for the importance of 15-lipoxygenase for the in vivo degradation of mitochondria. We find that inhibitor treatment retarded mitochondrial degradation, as shown by persistence of marker proteins and by direct visualization of mitochondria by electron microscopy.
Molecular Pharmacology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.
C
C
Grüllich
R M
RM
Duvoisin
M
M
Wiedmann
K
K
van Leyen
D000818:Animals; D001093:Arachidonate 15-Lipoxygenase / Q000378:metabolism*; D001095:Arachidonic Acids / Q000494:pharmacology; D015153:Blotting, Western; D048429:Cell Size / Q000187:drug effects; D003600:Cytosol / Q000201:enzymology / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects; D007425:Intracellular Membranes / Q000378:metabolism; D016859:Lipoxygenase Inhibitors; D015388:Organelles / Q000187:drug effects / Q000378:metabolism*; D011817:Rabbits; D012156:Reticulocytes / Q000187:drug effects / Q000201:enzymology*
D001095:Arachidonic Acids; D004791:Enzyme Inhibitors; D016859:Lipoxygenase Inhibitors; D001093:Arachidonate 15-Lipoxygenase