A light-responsive and periodic NADH oxidase activity of the cell surface of Tetrahymena and of human buffy coat cells.
Oxidation of external NADH (NADH is an impermeant substrate) by cells of Tetrahymena pyriformis oscillated with a period of 24-26 min. The period length in darkness (25.6 min) appeared to be slightly longer than the period in light (approximately 24 min). When Tetrahymena were placed in darkness for 30-50 min and then returned to light, a new maximum in the rate of NADH oxidation was observed 36-38 min (13 + 24) min after the beginning of the light treatment. The cell-surface NADH oxidase of human buffy coats (a mixture of white cells and platelets) also was periodic and light responsive.
Peter
A D
AD
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907-1333, USA.
Morré
D J
DJ
Morré
D M
DM
D000818:Animals; D001792:Blood Platelets / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism*; D003624:Darkness; D006801:Humans; D007962:Leukocytes / Q000378:metabolism; D008027:Light; D009097:Multienzyme Complexes / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D013053:Spectrophotometry; D013769:Tetrahymena pyriformis / Q000378:metabolism*; D013997:Time Factors
D009097:Multienzyme Complexes; D009243:NAD; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases; D010100:Oxygen