Determination of idarubicin in human urine by capillary zone electrophoresis with amperometric detection.
A simple, reliable and reproducible method, based on capillary zone electrophoresis with amperometric detection, has been developed for the determination of idarubicin in human urine. A carbon disk electrode was used as working electrode. The optimal conditions of separation and detection were pH 5.6 phosphate buffer (0.20 mol/L), 22 kV for the separation voltage and 1.00 V (vs. Ag/AgCl, 3 mol/L KCl) for the detection potential. The linear range was from 4.0 x 10(-7) to 2.0 x 10(-5) mol/L with a regression coefficient of 0.9986, and the detection limit was 8.0 x 10(-8) mol/L. The method was directly applied to the determination of idarubicin in spiked human urine without any other sample pretreatment except filtration, and the assay results were satisfactory.
Hu
Q
Q
Department of Chemistry, East China Normal University, Shang hai. yuzhi@online.sh.cn
Zhou
T
T
Zhang
L
L
Li
H
H
Fang
Y
Y
D000903:Antibiotics, Antineoplastic / Q000652:urine*; D004563:Electrochemistry; D004566:Electrodes; D019075:Electrophoresis, Capillary; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015255:Idarubicin / Q000652:urine*; D012044:Regression Analysis
D000903:Antibiotics, Antineoplastic; D015255:Idarubicin