Effects of lipopolysaccharide on interleukin-6 production in perfused human placental cotyledons.
OBJECTIVE
To determine if lipopolysaccharide (LPS) alters production of interleukin-6 (IL-6) or vascular tone in perfused placental cotyledons.


METHODS
Control and study cotyledons from nine placentas were perfused for 3 h. Study cotyledons received LPS in concentrations of 0.01 mcg/ml (n = 3), 0.1 mcg/ml (n = 3), or 1.0 mcg/ml (n = 3). Effluents were collected at 30, 60, 120, and 180 min following infusion with LPS. IL-6 concentrations were measured by enzyme-linked immunosorbant assay. Perfusion pressures were recorded at 10-min intervals. Data were analyzed using ANOVA for repeated measures.


RESULTS
IL-6 production significantly increased over time in both the study and control cotyledons (P = 0.002). LPS treatment did not affect IL-6 production (P = 0.85) and there were no observable dose effects (P = 0.13). Perfusion pressures did not differ (P = 0.16).


CONCLUSIONS
The isolated perfused placental cotyledon produces IL-6 and concentrations increase over time. LPS does not alter production of IL-6 or fetoplacental vascular tone.
Department of Obstetrics and Gynecology, Madigan Army Medical Center, Tacoma, Washington, USA. MajWagner@aol.com
R K
RK
Wagner
R M
RM
Hinson
C C
CC
Apodaca
N
N
Hoeldtke
T
T
Buchanan
R F
RF
Hume
B C
BC
Calhoun
D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis*; D007700:Kinetics; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D010477:Perfusion; D010920:Placenta / Q000098:blood supply* / Q000187:drug effects / Q000378:metabolism*; D011247:Pregnancy
D015850:Interleukin-6; D008070:Lipopolysaccharides