Localization of a new neutralizing epitope on the mumps virus hemagglutinin-neuraminidase protein.
Four protein fragments which span the entire hemagglutinin-neuraminidase protein (HN) of mumps virus were expressed in HeLa cells and cell extracts were tested for their capability to induce neutralizing antibodies in mice. Fragment HN3 (aa 213-372) was able to induce the production of hemagglutination-inhibiting and neutralizing antibodies. When a subfragment of HN3, the synthetic peptide NSTLGVKSAREF (aa 329-340 of HN) was used for immunization, hemagglutination-inhibiting and neutralizing antibodies against mumps wild type virus but not against the Urabe Am9 vaccine virus were raised. The peptide could, therefore, contain a new epitope, which may be critical for protective host humoral immune response.
Cusi
M G
MG
Department of Molecular Biology, Section of Microbiology, University of Siena, Via Laterina, 8-53100, Siena, Italy. cusi@unisi.it
Fischer
S
S
Sedlmeier
R
R
Valassina
M
M
Valensin
P E
PE
Donati
M
M
Neubert
W J
WJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral; D002460:Cell Line; D002522:Chlorocebus aethiops; D018604:Epitope Mapping; D018985:Epitopes, B-Lymphocyte / Q000032:analysis* / Q000276:immunology; D005260:Female; D015707:HN Protein / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009108:Mumps Vaccine; D009109:Mumps virus / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D009439:Neuraminidase / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D009500:Neutralization Tests; D010455:Peptides / Q000032:analysis / Q000138:chemical synthesis; D014709:Vero Cells
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D018985:Epitopes, B-Lymphocyte; D015707:HN Protein; D009108:Mumps Vaccine; D010455:Peptides; D009439:Neuraminidase