[The effect of cytokines on the activation of T cells induced by B7-1-transfected leukemia cells].
OBJECTIVE
To observe the effects of IL-12, IFN-gamma, IL-10 on the B7-1 expression of B7-1 transfected leukemia cells (K562 and HL-60) and on the IL-2 and IFN-gamma expression and production in the peripheral blood mononuclear cells(PBMC) induced by B7-1 transfected K562 and HL-60 cells.


METHODS
RT-PCR and ELISA techniques were used to assay the expression and production of IFN-gamma and IL-2.


RESULTS
IFN-gamma and IL-12 increased the expressions of B7-1, IL-2 and IFN-gamma, but IL-10 inhibited all the expressions.


CONCLUSIONS
IFN-gamma, IL-10 and IL-12 affected the expression and production of IFN-gamma and IL-2 possibly by affecting the B7-1 expression.
Children's Hospital, Chongqing University of Medical Sciences, Chongqing 400014.
G
G
Huang
C
C
Li
X
X
Yang
D018122:B7-1 Antigen; D015870:Gene Expression; D018922:HL-60 Cells / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D018664:Interleukin-12 / Q000494:pharmacology*; D007376:Interleukin-2 / Q000378:metabolism; D020014:K562 Cells / Q000378:metabolism; D007938:Leukemia / Q000235:genetics* / Q000276:immunology; D008213:Lymphocyte Activation; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection
D018122:B7-1 Antigen; D007376:Interleukin-2; D012333:RNA, Messenger; D018664:Interleukin-12; D007371:Interferon-gamma