[The relation between DNA replication error and clinicopathological features of colorectal carcinoma].
OBJECTIVE
To study the relationship between DNA replication error and development of colorectal carcinoma (CRC).


METHODS
Silver staining PCR-SSCP and denatured polyacrylamide gel electrophoresis methods were used to detect microsatellites instability (MSI) at 4 loci on chromosome 2, 5, 17 in paraffin embedded specimens of 60 CRC and their paired normal tissue. If replication errors (RER) were found at 2 or more loci, then a diagnosis of RER+ was made.


RESULTS
RER+ was observed in 19/60 CRC, among which 7 cases had CRC family history. According to Amsterdam criteria, 4 were diagnosed as HNPCC(hereditary nonpolyposis colorectal cancer), of which 3 cases were RER+. The ratio of RER+ in HNPCC (75%) was significantly higher than that among sporadic CRC (28.5%). Most RER+ CRC have features of poorly differentiated adenocarcinoma (P < 0.01), tendency for right side involvement of the colon (P < 0.05) and a higher proportion with family history (P < 0.05). Rate of Dukes A and B stage higher than C and D stage (P < 0.05).


CONCLUSIONS
RER+ is a relatively common molecular event in CRC. RER+ CRC and RER- CRC have different clinicopathological features and behavior.
Department of Pathology, First Military Medical University, Guangzhou 510515.
N
N
Xu
H
H
Qiu
Y
Y
Ding
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D002288:Adenocarcinoma, Mucinous / Q000235:genetics / Q000473:pathology; D000231:Adenocarcinoma, Papillary / Q000235:genetics / Q000473:pathology; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology; D004261:DNA Replication; D004273:DNA, Neoplasm / Q000235:genetics; D006801:Humans; D018895:Microsatellite Repeats; D012004:Rectal Neoplasms / Q000235:genetics* / Q000473:pathology
D004273:DNA, Neoplasm