Arginine vasopressin inhibits apoptosis of rat glomerular mesangial cells via V1a receptors.
Arginine vasopressin (AVP) promotes proliferation of glomerular mesangial cells. We examined whether AVP modulates an apoptosis of cultured rat glomerular mesangial cells at 3-17th passages. The agarose gel electrophoresis demonstrated that AVP attenuated a ladder formation stimulated by the serum deprivation. The quantitation of oligonucleosomes by ELISA also showed that AVP suppressed the serum deprivation-induced apoptosis. Such an antiapoptotic effect of AVP was dose-dependent. An AVP V1a receptor antagonist, d(CH2)5Tyr(Me)AVP, abolished the antiapoptotic effect of AVP. The inhibitory effect of AVP on the apoptosis was reduced by staurosporine and mimicked by phorbol-12-myristate-13-acetate. These results suggest that AVP inhibits serum deprivation-induced apoptosis of glomerular mesangial cells via V1a receptor-protein kinase C pathway.
Department of Medicine, Jichi Medical School, Tochigi, Japan.
M
M
Higashiyama
S
S
Ishikawa
T
T
Saito
T
T
Nakamura
I
I
Kusaka
S
S
Nagasaka
K
K
Honda
T
T
Saito
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001127:Arginine Vasopressin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D002478:Cells, Cultured; D004587:Electrophoresis, Agar Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005920:Glomerular Mesangium / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D007678:Kidney Glomerulus; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D017483:Receptors, Vasopressin / Q000378:metabolism*; D019311:Staurosporine / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D017483:Receptors, Vasopressin; D001127:Arginine Vasopressin; C025043:vasopressin, 1-(1-mercaptocyclohexaneacetic acid)-2-(O- methyl-L-tyrosine)-8-L-arginine-; C068622:argipressin,(1-mercaptocyclohexaneacetic acid)(1)-O-ethyl-Tyr(2)-; D019311:Staurosporine; D013755:Tetradecanoylphorbol Acetate