Rapid identification of Candida glabrata based on trehalose and sucrose assimilation using Rosco diagnostic tablets.
We developed a simple method for the identification of Candida glabrata on the basis of the ability of this species to rapidly assimilate trehalose but not sucrose. After incubation of yeasts with Rosco diagnostic tablets containing sucrose or trehalose, identification of C. glabrata was achieved in 4 h with 100% sensitivity and specificity.
Laboratoire de Parasitologie et Mycologie, Hôpital du Bocage, 21034 Dijon Cedex, France.
J
J
Lopez
F
F
Dalle
P
P
Mantelin
P
P
Moiroux
A C
AC
Nierlich
A
A
Pacot
B
B
Cuisenier
O
O
Vagner
A
A
Bonnin
D002175:Candida / Q000145:classification* / Q000378:metabolism*; D002177:Candidiasis / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D016533:Mycological Typing Techniques; D012680:Sensitivity and Specificity; D013395:Sucrose / Q000378:metabolism*; D013607:Tablets; D014199:Trehalose / Q000378:metabolism*
D013607:Tablets; D013395:Sucrose; D014199:Trehalose