Detection of genes for alkane and naphthalene catabolism in Rhodococcus sp. strain 1BN.
Rhodococcus sp. 1BN was isolated from a contaminated site and showed various biodegradative capabilities. Besides naphthalene, strain 1BN degraded medium- (C6) and long-chain alkanes (C16-C28), benzene and toluene, alone or when the hydrocarbons were mixed in equal proportions. The nucleotide sequence of an alk polymerase chain reaction (PCR) fragment revealed a 59% nucleotide homology to the Pseudomonas oleovorans alkB gene. The nar fragments were highly homologous to genes coding for large and small subunits of cis-naphthalene 1,2-dioxygenase (narAa and narAb) and to cis-naphthalene dihydrodiol dehydrogenase (narB) from other rhodococci. The oxidation of indene to cis-(1S,2R)-1,2-dihydroxyindan by toluene-induced cells allows to hypothesize that strain 1BN also carries a toluene dioxygenase-like system.
Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Università degli Studi di Milano, Milan, Italy. vincenza.andreoni@unimi.it
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Andreoni
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Bernasconi
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Colombo
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JB
van Beilen
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Cavalca
D000473:Alkanes / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D042926:Cytochrome P-450 CYP4A; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D005798:Genes, Bacterial; D007192:Indenes / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009281:Naphthalenes / Q000378:metabolism*; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000235:genetics / Q000378:metabolism; D010084:Oxidation-Reduction; D010105:Oxygenases / Q000378:metabolism; D021122:Protein Subunits; D011549:Pseudomonas / Q000235:genetics* / Q000378:metabolism; D012240:Rhodococcus / Q000235:genetics* / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D014050:Toluene / Q000378:metabolism
D000473:Alkanes; D001426:Bacterial Proteins; D007192:Indenes; D009281:Naphthalenes; D021122:Protein Subunits; C092057:narA protein, Bacillus subtilis; C031721:naphthalene; D014050:Toluene; C093581:indene; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D010105:Oxygenases; C023172:toluene dioxygenase; D042926:Cytochrome P-450 CYP4A; D009565:Nitrate Reductases; D050901:Nitrate Reductase