Treatment with 815-nm diode laser induces long-lasting expression of 72-kDa heat shock protein in normal rat skin.
BACKGROUND
We previously reported that skin closure is improved by photoirradiation of the wound margins with an 815-nm diode laser system.


OBJECTIVE
To determine whether the beneficial effects of laser treatment involve the overexpression of the inducible 72-kDa heat shock protein, Hsp70.


METHODS
Expression of Hsp70 was investigated by immunocytochemistry in normal hairless rat dorsal skin and compared with its expression after laser photoirradiation.


RESULTS
Constitutive expression of Hsp70 was mainly confined to the upper epidermal layer. Laser irradiation further increased epidermal expression of Hsp70 while inducing de novo synthesis of the protein in dermal structures, particularly around blood vessels, hair follicles and sebaceous glands. Laser-induced expression of Hsp70 was still present 7 days after photoirradiation.


CONCLUSIONS
Laser-induced expression of Hsp70 might contribute to improved tissue regeneration and wound healing.
Laboratoire de Physiologie Respiratoire et de Biologie Cellulaire, UFR Cochin Port-Royal, 24 rue du Faubourg Saint-Jacques, 75014 Paris, France.
E
E
Souil
A
A
Capon
S
S
Mordon
A T
AT
Dinh-Xuan
B S
BS
Polla
M
M
Bachelet
D000818:Animals; D004817:Epidermis / Q000378:metabolism / Q000528:radiation effects; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000378:metabolism; D053685:Laser Therapy; D008297:Male; D051381:Rats; D012867:Skin / Q000378:metabolism / Q000528:radiation effects*; D014945:Wound Healing / Q000502:physiology / Q000528:radiation effects
D018840:HSP70 Heat-Shock Proteins; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins