Quantification and in situ localization of MCP-1 mRNA and its relation to the immune response of renal cell carcinoma.
Malignant tumours are usually accompanied by an immune response. Chemokines such as MCP-1 have been claimed to be potent inducers of such tumour-associated reactions. In the present study MCP-1 mRNA was quantified by competitive reverse transcription polymerase reaction and localised by in situ hybridisation in renal cell carcinoma tissue in comparison to tumour-free tissue of the same nephrectomy specimen. MCP-1 mRNA levels were correlated with the immune cell infiltrate, the density of CD31(+)microvessels, and the endothelial expression of ICAM-1, VCAM-1, E-, and P-selectin. In only seven of 19 cases, MCP-1 mRNA levels in carcinoma tissue were increased in comparison to tumour-free tissue. Within tumour tissue, mRNA transcripts could be localised in tumour cells, microvessel endothelia, and in tumour-associated macrophages. A correlation between MCP-1 mRNA levels and the density of immune cells, especially macrophages, the microvessel density, and the expression of adhesion molecules could not be observed. Therefore, MCP-1 seems to be of minor importance for the induction of an immune response in renal cell carcinomas regarding at least the parameters analysed in this study.
Department of Pathology, University of Göttingen, Robert-Koch-Str. 40, D-37075 Göttingen, Germany. hemmer@med.uni-goettingen.de
B
B
Hemmerlein
U
U
Johanns
A
A
Kugler
M
M
Reffelmann
H J
HJ
Radzun
D002292:Carcinoma, Renal Cell / Q000098:blood supply / Q000737:chemistry / Q000276:immunology* / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000096:biosynthesis; D018932:Chemokine CCL2 / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D017403:In Situ Hybridization; D007668:Kidney / Q000098:blood supply / Q000737:chemistry / Q000276:immunology / Q000473:pathology; D007680:Kidney Neoplasms / Q000098:blood supply / Q000737:chemistry / Q000276:immunology* / Q000473:pathology; D008833:Microcirculation / Q000276:immunology / Q000473:pathology; D020556:Neutrophil Infiltration / Q000276:immunology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism*
D015815:Cell Adhesion Molecules; D018932:Chemokine CCL2; D012333:RNA, Messenger