An amperometric cellobiose dehydrogenase-based biosensor can be used for measurement of cellulase activity.
The hemoflavoenzyme cellobiose dehydrogenase (CDH, EC 1.1.99.18) from Phanerochaete chrysosporium has been used in an amperometric redox polymer-based biosensor. Used in conjugation with a FIA system this biosensor can replace colorimetric assays for measuring cellobiose liberated from cellulose in a series of cellulase-containing samples. The biosensor gave the same result as the Somogyi-Nelson method in a less time-consuming and laborious manner. The two methods showed about the same precision.
Hildén
L
L
Department of Biochemistry, Uppsala University, SE-751 23 Uppsala, Sweden. lars.hilden@biokem.uu.se
Eng
L
L
Johansson
G
G
Lindqvist
S E
SE
Pettersson
G
G
D015374:Biosensing Techniques / Q000379:methods*; D002237:Carbohydrate Dehydrogenases / Q000737:chemistry*; D002480:Cellulase / Q000032:analysis*; D005069:Evaluation Studies as Topic; D020075:Phanerochaete / Q000201:enzymology; D011786:Quality Control; D015203:Reproducibility of Results; D013997:Time Factors
D002237:Carbohydrate Dehydrogenases; C019859:cellobiose-quinone oxidoreductase; D002480:Cellulase