[The expression, secretion and regulation of membrane-soluble syndecan-1 in human multiple myeloma cells].
OBJECTIVE
To analyze the expression, secretion and regulation of membrane-soluble syndecan-1 in human multiple myeloma cells, and to explore the relationship between syndecan-1 and the development of human multiple myeloma.


METHODS
Syndecan-1 expression on the surface of malignant plasma cells was analyzed by immuno-staining, the levels of soluble syndecan-1 in the supernatant of cultured tumor cells by ELISA.


RESULTS
1. Human multiple myeloma cells expressed high levels of syndecan-1. The density of syndecan-1 varied from 170,000 to 800,000 molecules per cell. The expression of syndecan-1 lost rapidly as the cells underwent apoptosis. 2. Most myeloma cells produced soluble syndecan-1, the rate of production did not correlate with the density of membrane syndecan-1. Moreover, the production of soluble syndecan-1 did not require serum. 3. The protein synthesis inhibitor cycloheximide inhibited the production of syndecan-1. The serine protease inhibitor antipain did not affect the secretion of syndecan-1.


CONCLUSIONS
As a receptor of many growth factors, cytokines and heparin-like growth factors, syndecan-1 might play an important role in tumor cell growth and homing in multiple myeloma development.
Li
X
X
Department of Immunology, Suzhou University, Suzhou 215007, China.
Lu
Z
Z
Klein
B
B
D006801:Humans; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000378:metabolism / Q000502:physiology*; D009101:Multiple Myeloma / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D011509:Proteoglycans / Q000096:biosynthesis / Q000378:metabolism / Q000502:physiology*; D053668:Syndecan-1; D053667:Syndecans; D014407:Tumor Cells, Cultured
D008562:Membrane Glycoproteins; D011509:Proteoglycans; C508241:SDC1 protein, human; D053668:Syndecan-1; D053667:Syndecans