[Membranotropic effects of electromagnetic radiation of extremely high frequency on Escherichia coli].
It was found that "sound" electromagnetic radiations of extremely high frequencies (53.5-68 GHz) or millimeter waves (wavelength range of 4.2-5.6 mm) of low intensity (power density 0.01 mW) have a bactericidal effect on Escherichia coli bacteria. It was shown that exposure to irradiation of extremely high frequencies increases the electrokinetic potential and surface change density of bacteria and decreases of membrane potential. The total secretion of hydrogen ions was suppressed, the H+ flux from the cytoplasm to medium decreased, and the flux of N,N'-dicyclohexylcarbodiimide-sensitive potassium ions increased, which was accompanied by changes in the stoichiometry of these fluxes and an increase in the sensitivity of H+ ions to N,N'-dicyclohexylcarbodiimide. The effects depended on duration of exposure: as the time of exposure increased, the bactericidal effect increased, whereas the membranotropic effects decreased. The effects also depended on growth phase of bacteria: the irradiation affected the cells in the stationary but not in the logarithmic phase. It is assumed that the H(+)-ATPase complex F0F1 is involved in membranotropic effects of electromagnetic radiation of extremely high frequencies. Presumably, there are some compensatory mechanisms that eliminate the membranotropic effects.
Yerevan State University, ul. A. Manukyana 1, Yerevan, 375049 Armenia.
A
A
Trchunian
E
E
Ogandzhanian
E
E
Sarkisian
S
S
Gonian
A
A
Oganesian
S
S
Oganesian
D002118:Calcium / Q000378:metabolism; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology; D004560:Electricity; D004926:Escherichia coli / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects*; D005947:Glucose / Q000494:pharmacology; D017136:Ion Transport; D008564:Membrane Potentials; D008872:Microwaves; D009997:Osmotic Pressure; D011188:Potassium / Q000378:metabolism; D013395:Sucrose / Q000494:pharmacology
D004024:Dicyclohexylcarbodiimide; D013395:Sucrose; D005947:Glucose; D011188:Potassium; D002118:Calcium