Ferrocenecarboxaldehyde labeled DNA probe for the study on DNA damage and protection.
A ferrocenecarboxaldehyde (FCA) labeled DNA probe is used for the first time in the study of DNA damage and protection. The electrochemically active reagent FCA was labeled successfully on to a denatured calf-thymus DNA by 1-ethyl-3- (3-dimethyl-aminopropyl) carbodiimide (EDC). The FCA labeled DNA probe was used to hybridize with the sample DNA sequence accumulated on the surface of a graphite electrode. The anodic peaks of the FCA bound to the double-stranded DNA (dsDNA) by differential pulse voltammetry (DPV) were used for the detection of DNA damage and protection. Thiourea, sodium benzoic acid and isopropanol can decrease DNA damage by hydroxyl radicals, and their protection efficiencies are discussed.
Xu
C
C
Department of Chemistry, East China Normal University, Shanghai, P.R. China.
Cai
H
H
He
P
P
Fang
Y
Y
D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D004249:DNA Damage; D015342:DNA Probes; D004563:Electrochemistry / Q000379:methods; D005022:Ethyldimethylaminopropyl Carbodiimide; D005296:Ferrous Compounds; D007202:Indicators and Reagents; D009691:Nucleic Acid Denaturation; D011199:Potentiometry; D013950:Thymus Gland
D015342:DNA Probes; D005296:Ferrous Compounds; D007202:Indicators and Reagents; C422706:ferrocenecarboxaldehyde; D004247:DNA; D005022:Ethyldimethylaminopropyl Carbodiimide