121R protein from the E3 region of bovine adenovirus-3 inhibits cytolysis of mouse cells by human tumor necrosis factor.
Based on the DNA sequence and known mRNA structures, the early region 3 (E3) of bovine adenovirus (BAV)-3 has the potential to encode four proteins. One of them (121R) is produced as a 14.5-kD protein throughout infection. Analysis of the 121R protein showed limited homology to a 14.7K protein of human adenovirus (HAV)-5. Interestingly, both anti-14.7K and anti-121R sera immunoprecipitated a 14.5-kD protein from cells infected with BAV-3. To determine if 121R is functionally related to 14.7K, we constructed a recombinant E3-deleted HAV-5 (AdKV121) expressing the BAV-3 E3 121R protein. Mouse C3HA cells infected with HAV-5 mutant dl 758 (deletion of 14.7K) were sensitive to TNF lysis. However, wild-type HAV-5- or recombinant AdKV121-infected cells were resistant to TNF-induced cytolysis. Our result show that the BAV-3 E3 121R protein is serologically and functionally related to the 14.7K protein encoded by the E3 region of HAV-5.
Veterinary Infectious Disease Organization, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
A N
AN
Zakhartchouk
D L
DL
Godson
L A
LA
Babiuk
S K
SK
Tikoo
D000256:Adenoviridae / Q000737:chemistry* / Q000502:physiology; D017503:Adenovirus E3 Proteins / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002470:Cell Survival; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D017353:Gene Deletion; D006801:Humans; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D016415:Sequence Alignment; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000494:pharmacology
D017503:Adenovirus E3 Proteins; D014409:Tumor Necrosis Factor-alpha