Synthesis of Hemolymph High-Density Lipoprotein beta-Glucan Binding Protein by Penaeus vannamei Shrimp Hepatopancreas.
It was recently shown that the shrimp high-density lipoprotein (HDL) and the beta-1,3-glucan binding protein (BGBP) are identical, implying dual functions for the same protein: lipid transport and involvement in the defense system. Because this protein is present in plasma, and the hepatopancreas is a major lipid storage gland, we investigated the presence of the HDL/BGBP polypeptide and its messenger RNA in this tissue using a monospecific antibody against HDL/BGBP. Hepatopancreas crude protein extracts, as well as polypeptides produced by poly(A)(+) RNA in vitro translation, were recognized by the anti-HDL/BGBP. Furthermore, a specific pattern was revealed in hepatopancreas thin sections by immunodetection. Strong recognition was seen in the epithelial cells of hepatopancreatic tubules, probably related to the secretion process of this protein.
Centro de Investigación en Alimentación y Desarrollo, P.O. Box 1735, Hermosillo, Sonora 83,000, Mexico
Gloria
G
Yepiz-Plascencia
Teresa Gollas
TG
Galván
Francisco
F
Vargas-Albores
Mónica
M
García-Bañuelos