Use of zwitterionic micelles in the eluent: a new approach for ion chromatographic (IC) analysis of ions in biological fluids with direct sample injection.
The problem of column performance degradation due to irreversible binding of proteins encountered in ion chromatographic (IC) analysis of ions in protein-containing samples was overcome by using zwitterionic micelles (e.g., Zwittergent-3-14) as a portion of the eluent. A zwitterionic micellar eluent showed high ability for solubilization of proteins, and, hence, the protein-containing samples could be analyzed without need for deproteinization. On the other hand, the zwitterionic micelle was insensitive to conductivity but interacted with the analyte ions, due mainly to its unique configuration of charges (namely, the zwitterionic micelle containing both positively and negatively charged groups but carrying no net charge). Using a zwitterionic micellar eluent, the analyte ions could be detected selectively and sensitively, and moreover, the selectivity for the analyte ions was unique. A conventional anion-exchange column conditioned with a Zwittergent-3-14 micellar eluent was applied for the analysis of real biological samples (serum and urine) with direct sample injection. The results of the successful detection of inorganic anions (Cl-, SO4(2-), NO2-, Br-, and NO3-) have demonstrated the usefulness of this new IC approach for the analysis of biological samples.
Hu
W
W
Division of Chemistry, Graduate School of Science, Hokkaido University, Sapporo, Japan. hu@ees.hokudai.ac.jp
Hasebe
K
K
Tanaka
K
K
D000818:Animals; D000838:Anions / Q000032:analysis* / Q000378:metabolism; D001798:Blood Proteins / Q000378:metabolism; D002417:Cattle; D002852:Chromatography, Ion Exchange / Q000379:methods*; D003902:Detergents / Q000378:metabolism*; D006801:Humans; D008823:Micelles; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D015203:Reproducibility of Results; D014556:Urine / Q000737:chemistry
D000838:Anions; D001798:Blood Proteins; D003902:Detergents; D008823:Micelles; D011506:Proteins