Oxidative modification of ion channel activity of ryanodine receptor.
The ryanodine receptor (RyR) is involved in the physiological Ca2+ release from the sarcoplasmic reticulum in both skeletal and cardiac muscles. The redox regulation is a plausible endogenous regulatory mechanism of the RyR. Sulfhydryl oxidation or S-nitrosylation of the cardiac RyR has been reported to activate the channel. Our laboratory demonstrated that hydroxyl radicals also activate the cardiac Ca2+-release channel activity, likely through the modification of sulfhydryl groups of the RyR.
Bioregulation Research Group, National Institute of Radiological Sciences, Chiba, Japan. anzai@nirs.go.jp
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Anzai
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Ogawa
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Ozawa
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Yamamoto
D000818:Animals; D020013:Calcium Signaling / Q000187:drug effects* / Q000502:physiology; D002352:Carrier Proteins; D003545:Cysteine / Q000378:metabolism; D003553:Cystine / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000494:pharmacology; D017136:Ion Transport / Q000187:drug effects; D008051:Lipid Bilayers; D008954:Models, Biological; D009124:Muscle Proteins / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism / Q000648:ultrastructure; D009206:Myocardium / Q000378:metabolism / Q000648:ultrastructure; D009569:Nitric Oxide / Q000494:pharmacology; D009603:Nitroso Compounds / Q000494:pharmacology; D016877:Oxidants / Q000494:pharmacology; D010084:Oxidation-Reduction; D011487:Protein Conformation / Q000187:drug effects; D011817:Rabbits; D019163:Reducing Agents / Q000494:pharmacology; D019837:Ryanodine Receptor Calcium Release Channel / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D012519:Sarcoplasmic Reticulum / Q000187:drug effects* / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000378:metabolism
D002352:Carrier Proteins; D008051:Lipid Bilayers; D009124:Muscle Proteins; D009603:Nitroso Compounds; D016877:Oxidants; D019163:Reducing Agents; D019837:Ryanodine Receptor Calcium Release Channel; D013438:Sulfhydryl Compounds; C070048:triadin; D009569:Nitric Oxide; D017665:Hydroxyl Radical; D003553:Cystine; D006861:Hydrogen Peroxide; D003545:Cysteine