Rapid liquid chromatographic determination of residual penicillin G in milk.
A simple and rapid high-performance liquid chromatographic (HPLC) method for determination of residual penicillin G (benzylpenicillin, PCG) in milk was developed. The sample preparation was performed by stirring with ethanol and reacting with 5 M 1,2,4-triazole-mercury (II) chloride solution at 65 degrees C for 10 min followed by an ultra centrifugation step. The HPLC separation was carried out using a Mightysil RP-4GP column, a mobile phase of acetonitrile and 0.1 M phosphate buffer (pH 6.5) (35:65, v/v) and a photo-diode array detector. The average recoveries from spiked PCG (0.004, 0.01, 0.05 and 0.1 microgram/mL) were above 86% with coefficients of variation between 1.2 and 4.5%. The limit of detection was 0.004 microgram/mL. This value corresponds to the maximum residue limit (MRL) in milk (0.004 microgram/mL, EU and Japan). The total time required for the analysis of one sample was below 40 min.
Faculty of Human Life Science, Osaka City University, Osaka 558-8585, Japan. furusawa@life.osaka-cu.ac.jp
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D000818:Animals; D002138:Calibration; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004350:Drug Residues / Q000032:analysis*; D007202:Indicators and Reagents; D008892:Milk / Q000737:chemistry*; D010400:Penicillin G / Q000032:analysis*; D010406:Penicillins / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D007202:Indicators and Reagents; D010406:Penicillins; D010400:Penicillin G