Genetic reidentification of the pectinolytic yeast strain SCPP as Saccharomyces bayanus var. uvarum.
Using genetic hybridization analysis, pulsed-field gel electrophoresis of chromosomal DNA and PCR/RFLP analysis of the MET2 gene, we reidentified 11 Champagne yeast strains. Two of them, SCPP and SC4, were found to belong to Saccharomyces bayanus var. uvarum and the remaining strains to S. cerevisiae. Strain
State Institute for Genetics and Selection of Industrial Microorganisms, Moscow, Russia. gnaumov@yahoo.com
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Naumov
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Naumova
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Aigle
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Masneuf
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Belarbi
D004271:DNA, Fungal / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D005656:Fungal Proteins / Q000235:genetics; D016533:Mycological Typing Techniques; D009693:Nucleic Acid Hybridization / Q000379:methods; D010368:Pectins / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D012440:Saccharomyces / Q000145:classification* / Q000235:genetics* / Q000502:physiology; D014920:Wine / Q000382:microbiology*
D004271:DNA, Fungal; D005656:Fungal Proteins; D010368:Pectins