Antibacterial activity of a pepsin-derived bovine hemoglobin fragment.
Peptic digestion of bovine hemoglobin yields a fragment with antibacterial activity. This peptide was purified to homogeneity by a two-step procedure including anion exchange chromatography and preparative reversed-phase HPLC. Mass determination and fragmentation indicated that this peptide corresponded to the 1-23 fragment of the alpha chain of hemoglobin. The minimum inhibitory concentration and mode of action of this peptide towards Micrococcus luteus strain A270 were determined. Hemolytic assay, interaction with liposomes, and study of its structure in solution were also performed.
Laboratoire de Technologie des Substances Naturelles, Institut Universitaire de Technologie, Département Génie Biologique, Université des Sciences et Technologie de Lille, PO Box 179, 59653 Villeneuve d'Ascq Cedex, France.
R
R
Froidevaux
F
F
Krier
N
N
Nedjar-Arroume
D
D
Vercaigne-Marko
E
E
Kosciarz
C
C
Ruckebusch
P
P
Dhulster
D
D
Guillochon
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D006454:Hemoglobins / Q000737:chemistry*; D006461:Hemolysis; D006868:Hydrolysis; D008081:Liposomes / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D016982:Micrococcus luteus / Q000254:growth & development; D008969:Molecular Sequence Data; D010434:Pepsin A / Q000494:pharmacology*; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification*; D017433:Protein Structure, Secondary; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000900:Anti-Bacterial Agents; D006454:Hemoglobins; D008081:Liposomes; D010446:Peptide Fragments; D010434:Pepsin A