Characteristics of a recent isolate of Sarcocystis neurona (SN7) from a horse and loss of pathogenicity of isolates SN6 and SN7 by passages in cell culture.
An isolate of Sarcocystis neurona (SN7) was obtained from the spinal cord of a horse with neurologic signs. The parasite was isolated in cultures of bovine monocytes and equine spleen cells. The organism divided by endopolygeny and completed at least one asexual cycle in cell cultures in 3 days. The parasite was maintained by subpassages in bovine monocytes for 10 months when it was found to be non-pathogenic to gamma interferon knockout (KO) mice. Revival of a low passage (10th passage) of the initial isolate stored in liquid nitrogen for 18 months retained its pathogenicity for KO mice. Merozoites (10(6)) of the late passage (22nd passage) were infective to only one of four KO mice inoculated. Similar results were obtained with SN6 isolate of S. neurona. No differences were found in Western blot patterns using antigens from the low and high passage merozoites of the SN7 and SN6 isolates. These results suggest that prolonged passage in cell culture may affect the pathogenicity of some isolates of S. neurona.
Dubey
J P
JP
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Beltsville Agricultural Research Center, MD 20705-2350, USA. jdubey@anri.barc.usda.gov
Mattson
D E
DE
Speer
C A
CA
Hamir
A N
AN
Lindsay
D S
DS
Rosenthal
B M
BM
Kwok
O C
OC
Baker
R J
RJ
Mulrooney
D M
DM
Tornquist
S J
SJ
Gerros
T C
TC
D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis; D015153:Blotting, Western / Q000662:veterinary; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D006734:Horse Diseases / Q000469:parasitology*; D006736:Horses; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D012522:Sarcocystis / Q000302:isolation & purification* / Q000472:pathogenicity*; D012523:Sarcocystosis / Q000276:immunology / Q000662:veterinary*; D013116:Spinal Cord / Q000469:parasitology
D000913:Antibodies, Protozoan