Rapid determination of PAHs in soil samples by HPLC with fluorimetric detection following sonication extraction.
A rapid method for the determination of PAHs in soil samples based on their extraction with methylene chloride by sonication and subsequent separation by HPLC with fluorimetric detection is proposed. A Hypersil Green PAH column was used with a gradient of acetonitrile/water as the mobile phase, together with a program of nine excitation and emission wavelength pairs. Recoveries were in the range 70-98%, except for acenaphthene and naphthalene, at concentration levels 1.08-442 microg/kg with relative standard deviations in the range 2-15% (n = 4). Total PAHs found in soil samples were in the range 15-282 microg/kg. The results were compared with those obtained by applying the 3540 EPA method for two samples.
Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, Spain.
M N
MN
Kayali-Sayadi
S
S
Rubio-Barroso
C A
CA
Díaz-Díaz
L M
LM
Polo-Díez
D002138:Calibration; D002273:Carcinogens / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis*; D012015:Reference Standards; D012989:Soil Pollutants / Q000032:analysis*; D013010:Sonication; D013050:Spectrometry, Fluorescence
D002273:Carcinogens; D011084:Polycyclic Aromatic Hydrocarbons; D012989:Soil Pollutants