[Detection of MDM 2 and p 53 genes in rhabdomyosarcoma by in situ hybridization].
OBJECTIVE
To observe the correlation between MDM2, p53 genes and the pathogenesis, clinical pathology and prognosis in rhabdomyosarcoma (RMS).


METHODS
Thirty one cases of MRS with follow-up history were covered for this study. MDM2 and p53 genes were detected by digoxigenin labeling in situ hybridization-technique.


RESULTS
The positive rates for MDM2 and p53 genes were 77.4% (24/31) and 66.7% (21/31) respectively, regardless of age, sex and the histological typing of RMS, but there was remarkable difference in the positive rates and intensity between the well and poorly differentiated cases, metastatic and non-metastatic cases as well as one year and three years survival period (P < 0.05).


CONCLUSIONS
The detection of MDM2 and p53 gene is considered valuable in evaluating the malignant degree, predictable metastasis and prognosis of RMS.
Department of Pathology, Guangzhou General Hospital of PIA, Guangzhou 510010.
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R
Lai
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Z
Wang
X
X
Zhe
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D005260:Female; D005500:Follow-Up Studies; D015870:Gene Expression; D016158:Genes, p53; D006801:Humans; D017403:In Situ Hybridization; D007223:Infant; D008207:Lymphatic Metastasis; D008297:Male; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D009687:Nuclear Proteins; D011379:Prognosis; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis*; D051736:Proto-Oncogene Proteins c-mdm2; D012208:Rhabdomyosarcoma / Q000235:genetics* / Q000378:metabolism / Q000556:secondary
D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; C497206:MDM2 protein, human; D051736:Proto-Oncogene Proteins c-mdm2