Studies on cytotoxicity of nickel ions using C3H10T1/2 fibroblast cells.
Cytotoxicity of Ni ions were examined using C3H mouse derived 10T1/2 fibroblast cells. It became evident that Ni ions had dose-dependent cytotoxicity. The cell number incubated in DME medium containing 0.04 mM/L Ni ion for 6 days was reduced to half that in control DME medium without Ni. The cell totally disappeared in DME medium containing 2 mM/L Ni ion. The dissolution of Ni ions from Ni-containing metallic restorations must be lower than these concentration levels so that oral tissues might not be damaged. No neoplastic transformation was found on all cells examined.
Taira
M
M
Department of Dental Materials Science and Technology, Osaka University Faculty of Dentistry, Osaka, Japan. mtaira@iwate-med.ac.jp
Toguchi
M S
MS
Hamada
Y
Y
Okazaki
M
M
Takahashi
J
J
Ito
R
R
Toyosawa
S
S
Ijyuin
N
N
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects*; D007477:Ions; D007928:Lethal Dose 50; D051379:Mice; D008809:Mice, Inbred C3H; D009532:Nickel / Q000633:toxicity*; D017942:Nitric Acid / Q000737:chemistry; D017693:Sodium Bicarbonate / Q000737:chemistry
D003470:Culture Media; D007477:Ions; D017942:Nitric Acid; D009532:Nickel; D017693:Sodium Bicarbonate