[The role of bcl-2 gene family in taxol-mediated apoptosis in BJAB B cell lymphoma cell line].
OBJECTIVE
To study the apoptosis-inducing effect of taxol on BJAB B cell lymphoma cell line and the role of bcl-2 gene family.


METHODS
BJAB cells were treated with taxol and examined for morphologic changes under light and electron microscopes. Flow cytometry assays and DNA gel electrophoresis were used to demonstrate changes in nuclear DNA contents and DNA fragmentations. Changes in bcl-2 gene family at mRNA and protein levels were studied by immunohistochemistry and semi-quantitative RT-PCR technique.


RESULTS
Taxol inhibited growth of BJAB cells. The cells initially showed G2/M arrest, followed by apoptosis, in a time and dose dependent manner. The expression of bcl-2 at mRNA and protion level was decreased while that of bcl-xs transcription was increased after taxol treatment.


CONCLUSIONS
Taxol induces apoptosis of BJAB cells. Bcl-2 and bcl-xs are involved in the regulation of taxol-induced apoptosis.
Laboratory of Molecular Pathology, Cancer Hospital, Shanghai Medical University, Shanghai 200032, China.
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D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D015973:Gene Expression Regulation, Leukemic; D019254:Genes, bcl-2 / Q000502:physiology*; D006801:Humans; D016393:Lymphoma, B-Cell / Q000235:genetics* / Q000473:pathology; D017239:Paclitaxel / Q000008:administration & dosage / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000972:Antineoplastic Agents, Phytogenic; D017239:Paclitaxel