Voltammetric trace analysis of DNA and RNA.
A voltammetric determination of DNA/RNA is described. The new aspect is the use of the extracellular endonuclease Serratia marcescens in the sample preparation. Using this enzyme it is possible to determine DNA/ RNA with a detection limit of 2-5 pg/mL. This satisfies the requirements of the WHO and the FDA.
Technical University of Clausthal, Institute of Inorganic and Analytical Chemistry, Clausthal-Zellerfeld, Germany.
S
S
Reher
Y
Y
Lepka
G
G
Schwedt
D000818:Animals; D002138:Calibration; D002417:Cattle; D004247:DNA / Q000032:analysis*; D004563:Electrochemistry; D004720:Endonucleases; D005399:Fishes; D007202:Indicators and Reagents; D008099:Liver / Q000737:chemistry; D008297:Male; D012313:RNA / Q000032:analysis*; D012706:Serratia marcescens / Q000737:chemistry*; D012996:Solutions; D013094:Spermatozoa / Q000737:chemistry; D013950:Thymus Gland / Q000737:chemistry
D007202:Indicators and Reagents; D012996:Solutions; D012313:RNA; D004247:DNA; D004720:Endonucleases