Identification of proacrosin binding protein sp32 precursor as a human cancer/testis antigen.
Serological expression cloning of antigens eliciting a humoral immune response to a syngeneic mouse sarcoma identified pem (mouse placenta and embryonic expression gene) as a new member of the cancer/testis family. To identify the human homologue of pem, mouse pem sequences and pem-related expressed sequence tags from human testis were used as PCR primers for amplification using human testis cDNA. However, rather than pem, another gene, designated OY-TES-1, was isolated and found to be the human homologue of proacrosin binding protein sp32 precursor originally identified in mouse, guinea pig, and pig. OY-TES-1 maps to chromosome 12p12-p13 and contains 10 exons. Southern blot analysis suggests the presence of two OY-TES-1-related genes in the human genome. In normal tissues, OY-TES-1 mRNA was expressed only in testis, whereas in malignant tissues, a variable proportion of a wide array of cancers, including bladder, breast, lung, liver, and colon cancers, expressed OY-TES-1. Serological survey of 362 cancer patients with a range of different cancers showed antibody to OY-TES-1 in 25 patients. No OY-TES-1 sera reactivity was found in 20 normal individuals. These findings indicate that OY-TES-1 is an additional member of the cancer/testis family of antigens and that OY-TES-1 is immunogenic in humans.
Ono
T
T
Department of Immunology, Okayama University Medical School, Okayama 700-8558, Japan. onot@med.okayama-u.ac.jp
Kurashige
T
T
Harada
N
N
Noguchi
Y
Y
Saika
T
T
Niikawa
N
N
Aoe
M
M
Nakamura
S
S
Higashi
T
T
Hiraki
A
A
Wada
H
H
Kumon
H
H
Old
L J
LJ
Nakayama
E
E
D000595:Amino Acid Sequence; D000818:Animals; D000912:Antibodies, Neoplasm / Q000097:blood / Q000276:immunology; D000951:Antigens, Neoplasm / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D015139:Blotting, Southern / Q000379:methods; D002352:Carrier Proteins / Q000235:genetics / Q000276:immunology*; D002874:Chromosome Mapping / Q000379:methods; D002881:Chromosomes, Human, Pair 12; D018076:DNA, Complementary; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000235:genetics / Q000276:immunology; D011498:Protein Precursors / Q000235:genetics / Q000276:immunology*; D017386:Sequence Homology, Amino Acid; D013552:Swine; D013737:Testis / Q000276:immunology*
C485651:ACRBP protein, human; D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D002352:Carrier Proteins; D018076:DNA, Complementary; D047908:Intracellular Signaling Peptides and Proteins; D011498:Protein Precursors; C121113:proacrosin-binding protein, Sus scrofa