Monofunctional derivatives of coproporphyrins for phosphorescent labeling of proteins and binding assays.
p-Isothiocyanatophenyl derivatives of Pt(II)- and Pd(II)-coproporphyrin I are described as stable monofunctional reagents which enable simple covalent labeling of proteins and other biomolecules under mild conditions in aqueous solutions. Labeling procedure was optimized for antibodies, avidin, and neutravidin. Photophysical properties of resulting conjugates important for their use in binding assays based on time-resolved phosphorescence detection were studied. The functional activity and long-term storage stability of antibody conjugates were assessed in comparison with unmodified proteins. The new labels and their conjugates were evaluated in the solid-phase immunoassays using commercial time-resolved phosphorescence readers Victor(2) and Arcus-1230 (Wallac). Potential applications of these reagents in in vitro diagnostics are discussed.
O'Riordan
T C
TC
Biochemistry Department, National University of Ireland, Cork, Lee Maltings, Prospect Row, Cork, Ireland.
Soini
A E
AE
Papkovsky
D B
DB
D001360:Avidin / Q000032:analysis / Q000737:chemistry; D003306:Coproporphyrins / Q000032:analysis* / Q000737:chemistry; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis / Q000737:chemistry; D007700:Kinetics; D008163:Luminescent Measurements; D008665:Metalloporphyrins / Q000737:chemistry; D019809:Streptavidin / Q000032:analysis / Q000737:chemistry; D013997:Time Factors
D003306:Coproporphyrins; D007074:Immunoglobulin G; D008665:Metalloporphyrins; C093483:neutravidin; D001360:Avidin; D019809:Streptavidin