Deletion of chromosomal region 13q14.3 in childhood acute lymphoblastic leukemia.
Deletion of the 13q14 chromosomal region is frequent in B cell chronic lymphocytic leukemia (B-CLL) and is believed to inactivate a tumor supressor gene (TSG) next to RB1. We studied microsatellite markers spanning the 13q14 chromosomal region in 138 children with acute lymphoblastic leukemia (ALL). Allelic loss was demonstrated in six cases (4.3%). Deletion did not include RB1 in two cases. In five patients, the deleted region overlapped that described in B-CLL. A sixth patient harbored a smaller deletion, slightly more telomeric than minimal deleted regions reported in B-CLL. Apparent differences in the delineation of the minimal deleted region could be due to the fact that the putative TSG is a very large gene, with some deletions affecting only a part of it. Our present findings suggest that at least some of its exons lie within a region of less than 100 kb more telomeric that previously thought.
Laboratoire de Biochimie Génétique, H pital Robert Debré, INSERM U409, Faculté de Médecine Xavier Bichat, Paris, France.
H
H
Cavé
H
H
Avet-Loiseau
I
I
Devaux
G
G
Rondeau
P
P
Boutard
E
E
Lebrun
F
F
Méchinaud
E
E
Vilmer
B
B
Grandchamp
D000293:Adolescent; D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D002872:Chromosome Deletion; D002882:Chromosomes, Human, Pair 13; D017931:DNA Primers; D005838:Genotype; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007223:Infant; D016133:Polymerase Chain Reaction; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*
D017931:DNA Primers