Preliminary study of cytogenetic damage in personnel exposed to anesthetic gases.
Occupational exposure to anesthetic gases is associated with various adverse health effects. Genetic material has been shown to be a sensitive target of numerous harmful agents. The aim of this study was to examine whether chromosomal damage could serve to indicate exposure to anesthetics. A group of 43 hospital workers of three professions (anesthesiologists, technicians and operating room nurses) and 26 control subjects were examined for chromosome aberrations, sister chromatid exchanges and micronucleus frequency. The exposed groups matched in duration of exposure to anesthetics, but not in age. An equal ratio between women and men was possible in all groups except nurses. Likewise, the ratio between smokers and non-smokers was also not comparable. An increase in chromosome damage was found in all exposed groups. While the increase in sister chromatid exchange frequency was not significant, chromosome aberrations and micronucleus frequency increased significantly, showing higher rates in women. The results suggest that the micronucleus test is the most sensitive indicator of changes caused by anesthetic gases. The observed difference between sexes with respect to exposure risk call for further, targeted investigations.
Mutagenesis Unit, Institute for Medical Research and Occupational Health, Ksaverska c.2, HR-10001 Zagreb, Croatia. rrozgaj@imi.hr
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D000328:Adult; D000776:Anesthesiology; D018685:Anesthetics, Inhalation / Q000009:adverse effects*; D020732:Cytogenetic Analysis / Q000379:methods*; D004249:DNA Damage; D005260:Female; D006801:Humans; D019570:Inhalation Exposure / Q000009:adverse effects*; D008297:Male; D015162:Micronucleus Tests; D008875:Middle Aged; D016273:Occupational Exposure / Q000009:adverse effects* / Q000032:analysis*; D009871:Operating Room Nursing; D009872:Operating Room Technicians; D010865:Pilot Projects; D016012:Poisson Distribution; D012854:Sister Chromatid Exchange
D018685:Anesthetics, Inhalation