Use of functional proteomics to investigate PKC epsilon-mediated cardioprotection: the signaling module hypothesis.
The characterization of biological processes on the basis of alterations in the cellular proteins, or "proteomic" analysis, is a powerful approach that may be adopted to decipher the signaling mechanisms that underlie various pathophysiological conditions, such as ischemic heart disease. This review represents a prospectus for the implementation of proteomic analyses to delineate the myocardial intracellular signaling events that evoke cardioprotection against ischemic injury. In concert with this, the manifestation of a protective phenotype has recently been shown to involve dynamic modulation of protein kinase C-epsilon (PKC epsilon) signaling complexes (Ping P, Zhang J, Pierce WM Jr, and Bolli R. Circ Res 88: 59--62, 2001). Accordingly, "the signaling module hypothesis" is formulated as a plausible mechanism by which multipurpose stress-activated proteins and signaling kinases may function collectively to facilitate the genesis of cardioprotection.
Department of Physiology and Biophysics, University of Louisville and Department of Veterans Affairs, Louisville, Kentucky 40202-1783, USA.
T M
TM
Vondriska
J B
JB
Klein
P
P
Ping
D000818:Animals; D002316:Cardiotonic Agents; D006321:Heart / Q000502:physiology* / Q000503:physiopathology; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D008955:Models, Cardiovascular; D017202:Myocardial Ischemia / Q000503:physiopathology; D011493:Protein Kinase C / Q000378:metabolism*; D051744:Protein Kinase C-epsilon; D020543:Proteome / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*
D002316:Cardiotonic Agents; D007527:Isoenzymes; D020543:Proteome; C497255:PRKCE protein, human; D011493:Protein Kinase C; D051744:Protein Kinase C-epsilon