Identification of mouse Jun dimerization protein 2 as a novel repressor of ATF-2.
A mouse cDNA that encodes a DNA-binding protein was identified by yeast two-hybrid screening, using activating transcription factor-2 (ATF-2) as the bait. The protein contained a bZIP (basic amino acid-leucine zipper region) domain and its amino acid sequence was almost identical to that of rat Jun dimerization protein 2 (JDP2). Mouse JDP2 interacted with ATF-2 both in vitro and in vivo via its bZIP domain. It was encoded by a single gene and various transcripts were expressed in all tested tissues of adult mice, as well as in embryos, albeit at different levels in various tissues. Furthermore, mouse JDP2 bound to the cAMP-response element (CRE) as a homodimer or as a heterodimer with ATF-2, and repressed CRE-dependent transcription that was mediated by ATF-2. JDP2 was identified as a novel repressor protein that affects ATF-2-mediated transcription.
RIKEN, The Institute of Physical and Chemical Research, Tsukuba Institute, Tsukuba, Japan.
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Ugai
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Murata
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Horikoshi
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Yokoyama
D016475:3T3 Cells; D051698:Activating Transcription Factor 2; D000595:Amino Acid Sequence; D000818:Animals; D017362:Cyclic AMP Response Element-Binding Protein / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D015870:Gene Expression; D020868:Gene Silencing; D051379:Mice; D008969:Molecular Sequence Data; D012097:Repressor Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D020798:Two-Hybrid System Techniques
D051698:Activating Transcription Factor 2; C497106:Atf2 protein, mouse; D017362:Cyclic AMP Response Element-Binding Protein; C491386:Jundp2 protein, mouse; D012097:Repressor Proteins; D014157:Transcription Factors