Detection of chlamydial antibody by fetal serology--an aid to the diagnosis of ovine abortion.
Two serological tests (indirect immunofluorescence and enzyme-linked immunosorbent assay) were developed for the detection of fetal antibody to Chlamydia psittaci. Fetal blood and thoracic fluid from 126 field cases of suspected ovine chlamydial abortion were examined using both tests. Placenta and fetal tissues (lung, liver, and kidney) from the same animals were also examined by the following conventional diagnostic methods: isolation in McCoy cells, detection of chlamydial lipopolysaccharide (LPS), modified Ziehl-Nielsen staining, and direct fluorescent antibody staining of chlamydia in frozen cryostat sections. Seventy cases were positive by fetal serology, and of these, 68 were also positive by isolation and/or LPS detection. The remaining 56 cases had negative fetal serology, and of these, 39 were positive by isolation and/or LPS detection. Results indicate that fetal serology, although less sensitive than either isolation in McCoy cells or detection of chlamydial LPS antigen, may be of particular use when placenta is not available.
Kennedy
H E
HE
Department of Veterinary Science, The Queen's University of Belfast, Stormont, Northern Ireland.
McCullough
S J
SJ
Graham
D
D
Cassidy
J
J
Malone
F E
FE
Ellis
W A
WA
D000034:Abortion, Veterinary / Q000382:microbiology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D002691:Chlamydophila psittaci / Q000276:immunology / Q000302:isolation & purification*; D003937:Diagnosis, Differential; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005333:Fetus / Q000382:microbiology; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D009956:Psittacosis / Q000150:complications / Q000175:diagnosis / Q000662:veterinary*; D012680:Sensitivity and Specificity; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*
D000907:Antibodies, Bacterial