Determination of urinary methylated purine pattern by high-performance liquid chromatography.
We describe the group selective separation and quantification of unmodified and modified purines in human urine by high-performance reverse phase liquid chromatography. The pattern of oxypurines and methylated purines: hypoxanthine (Hx), xanthine (X), 1-methyl hypoxanthine (1-MHx), 1-methyl guanine (1-MG), 3-methyl guanine (3-MG), 7-methyl guanine (7-MG), 1-methyl xanthine (1-MX), 3-methyl xanthine (3-MX), 7-methyl xanthine (7-MX), 1,7-dimethyl guanine (1,7-dMG), 1,3-dimethyl xanthine (1,3-dMX), 1,7-dimethyl xanthine (3,7-dMX) and 1,3,7-trimethyl xanthine (1,3,7-tMX) were determined in a single run in urine of a healthy subject and a gout patient before and after treatment with allopurinol. This method may be useful to investigate the urinary pattern of methylated bases in diseases involving purine metabolism.
Institute of Biochemistry and Enzymology, University of Siena, Italy.
M C
MC
Di Pietro
D
D
Vannoni
R
R
Leoncini
G
G
Liso
R
R
Guerranti
E
E
Marinello
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006073:Gout / Q000378:metabolism; D006801:Humans; D008745:Methylation; D011687:Purines / Q000652:urine*
D011687:Purines