A simple colorimetric method to screen drug cytotoxicity against Leishmania using the dye Alamar Blue.
A quantitative colorimetric assay using the oxidation-reduction indicator Alamar Blue was developed to measure cytotoxicity of compounds against the protozoan parasite Leishmania. Absorbance increased linearly with the plating density of promastigotes of L. major MRHO/IR/76 vaccine strain up to at least 2.5 x 10(6) cells/ml when parasites were incubated for 72 h in the presence of 10% Alamar Blue. The 50% effective dose values of common drugs (amphotericin B, pentostam and paromomycin) obtained by this assay were in the same range as previously determined by other methods. The Alamar Blue assay permits a simple, reproducible and reliable method for screening antileishmanial drugs.
Mikus
J
J
Abteilung Parasitologie, Hygiene-Institut der Ruprecht-Karls-Universität, Im Neuenheimer Feld 324, D-69120 Heidelberg, Germany.
Steverding
D
D
D000666:Amphotericin B / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000967:Antimony Sodium Gluconate / Q000494:pharmacology; D000981:Antiprotozoal Agents / Q000494:pharmacology; D003124:Colorimetry / Q000379:methods*; D004396:Coloring Agents / Q000737:chemistry*; D018320:Leishmania major / Q000737:chemistry / Q000187:drug effects*; D016773:Leishmaniasis, Cutaneous / Q000188:drug therapy / Q000469:parasitology; D010078:Oxazines; D010084:Oxidation-Reduction; D021261:Parasitic Sensitivity Tests / Q000379:methods*; D010303:Paromomycin / Q000494:pharmacology; D015203:Reproducibility of Results; D014966:Xanthenes
D000900:Anti-Bacterial Agents; D000981:Antiprotozoal Agents; D004396:Coloring Agents; D010078:Oxazines; D014966:Xanthenes; C005843:resazurin; D010303:Paromomycin; D000666:Amphotericin B; D000967:Antimony Sodium Gluconate