Establishment of gene-vaccinated skin grafting against Toxoplasma gondii infection in mice.
Vaccine effects of in vivo gene-vaccinated skin graft were evaluated against Toxoplasma gondii (T. gondii) infection. By using a gene gun, cDNA coding T. gondii SAG1 molecule was intracutaneously vaccinated into C57BL/6 (B6; a susceptible strain), BALB/c (a resistant strain) and (C57BL/6 x BALB/c) F1 (CBF1) mice, and the gene-vaccinated skin of these strains was transplanted to CBF1 mice. Regarding the antibody production against SAG1, CBF1-recipient mice transplanted with the SAG1 gene-vaccinated B6 skin were high responders, whereas CBF1 mice skin grafted with vaccinated skin of both BALB/c and CBF1 mice were low responders. The donor-derived LC/DC migrated to the draining lymph nodes of the recipients from the skin graft within 3 days. The vaccine effect against T. gondii challenge infection was obtained in CBF1 mice which received the skin graft of the SAG1 gene-vaccinated BALB/c mice.
Saito
S
S
Department of Plastic and Reconstructive Surgery, Chiba University Hospital, Chiba University School of Medicine, 260-8670, Chiba, Japan.
Aosai
F
F
Rikihisa
N
N
Mun
H S
HS
Norose
K
K
Chen
M
M
Kuroki
T
T
Asano
T
T
Ochiai
T
T
Hata
H
H
Ichinose
M
M
Yano
A
A
D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics; D019470:Biolistics; D002465:Cell Movement / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology; D005260:Female; D007801:Langerhans Cells / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology; D016052:Protozoan Vaccines / Q000008:administration & dosage* / Q000235:genetics; D016038:Skin Transplantation / Q000276:immunology; D014122:Toxoplasma / Q000235:genetics* / Q000276:immunology*; D014123:Toxoplasmosis / Q000276:immunology* / Q000517:prevention & control*; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000235:genetics
D000953:Antigens, Protozoan; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; C047168:SAG1 antigen, Toxoplasma; D019444:Vaccines, DNA