Modulation of a lectin insecticidal activity by carbohydrates.
Lectins from plants present an insecticidal activity most probably through their carbohydrate binding properties; as a consequence, their toxicity should vary with the presence of a competitive sugar in the ingested food. In order to test this hypothesis, we performed competition experiments between insecticidal activity and carbohydrate binding. For this purpose, we used a lectin from Lathyrus ochrus and the specific carbohydrate for this protein, glucose. In toxicological tests with Drosophila melanogaster, we observed a decrease of lectin toxicity when glucose was added to the larva-rearing medium. This result suggests that the toxicity of the lectin is correlated to its ability to bind sugar in the insect digestive tract and stresses the importance of sugar composition of the nutriment used for toxicological testing of lectins or in genetically modified plants.
Université Paul Sabatier, Laboratoire de Synthèse et Physicochimie des Molécules d'Intérêt Biologique, UMR CNRS 5068, Groupe de Biochimie des Protéines, Toulouse, France.
V
V
Triguéros
M
M
Wang
D
D
Père
L
L
Paquereau
L
L
Chavant
D
D
Fournier
D000818:Animals; D050260:Carbohydrate Metabolism; D004331:Drosophila melanogaster / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D007306:Insecticides / Q000302:isolation & purification / Q000378:metabolism*; D037102:Lectins / Q000302:isolation & purification / Q000378:metabolism*; D037121:Plant Lectins
D007306:Insecticides; D037102:Lectins; D037121:Plant Lectins; C039412:lathyrus lectin; D005947:Glucose