[Studies on the in vivo induction of differentiation of the erythroleukemia cells by the antigen-pulsed dendritic cells modified with GM-CSF gene].
OBJECTIVE
To investigate the function and mechanism of dendritic cells in the immunotherapy for tumors.


METHODS
C57BL/6 mice inoculated with erythroleukemia cells were used as models. Inhibition or induction of tumor cell differentiation was observed by fluorescence-activated cell sorting (FACS) and transmission electron microscopy.


RESULTS
After pulsed with FBL-3 antigen, the GM-CSF gene transfected dendritic cells inhibited the proliferation of the tumor cells. The expression of CD14 was increased and the expressions of MHC-II, B7-1, B7-2 and VCAM-1 were also up-regulated. Erythroleukemia cells in different differentiating stages were observed in tumor tissues. Under transmission electron microscope, the volumes of FBL-3 cells were reduced, the amount of lysosome and hetero-chromatin became plentiful, the nucleocytoplasmic ratio was decreased, and apoptosis was revealed in erythroleukemia cells. The CD8+ cells were increased in peripheral blood and tumor tissues.


CONCLUSIONS
The antigen-pulsed dendritic cells modified with GM-CSF gene could induce in vivo the erythroleukemia cells to differentiate into monocytes.
Second Military Medical University, Shanghai 200433.
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Zhao
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Cao
D000260:Adenoviruses, Human / Q000235:genetics; D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D003713:Dendritic Cells / Q000276:immunology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics*; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation
D016178:Granulocyte-Macrophage Colony-Stimulating Factor