Characterization of high-level fluoroquinolone resistance in Escherichia coli O78:K80 isolated from turkeys.
Fluoroquinolone resistance was characterized in Escherichia coli O78:K80 isolated from diseased turkeys. The level of resistance to fluoroquinolones of the isolates appeared closely correlated with substitutions in GyrA and ParC, but not with the production of the AcrAB efflux pump. Among isolates highly resistant to ciprofloxacin (MIC 8 mg/L) and harbouring identical substitutions (two in GyrA and one in ParC), two close but distinguishable ribotypes were identified. This indicated that at least two independent selection events may have occurred.
Giraud
E
E
Station de Pathologie Aviaire et de Parasitologie, Institut National de la Recherche Agronomique, Centre de Recherche de Tours-Nouzilly, 37380 Monnaie, France.
Leroy-Sétrin
S
S
Flaujac
G
G
Cloeckaert
A
A
Dho-Moulin
M
M
Chaslus-Dancla
E
E
D019943:Amino Acid Substitution; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D002352:Carrier Proteins; D002939:Ciprofloxacin / Q000494:pharmacology; D027081:DNA Gyrase; D027101:DNA Topoisomerase IV; D004250:DNA Topoisomerases, Type II / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D004352:Drug Resistance, Microbial; D000077422:Enrofloxacin; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D029968:Escherichia coli Proteins; D024841:Fluoroquinolones; D008074:Lipoproteins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D026901:Membrane Transport Proteins; D008826:Microbial Sensitivity Tests; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D009154:Mutation; D009268:Nalidixic Acid / Q000494:pharmacology; D011807:Quinolizines / Q000494:pharmacology; D015363:Quinolones / Q000494:pharmacology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D014422:Turkeys / Q000382:microbiology*
D000890:Anti-Infective Agents; D001426:Bacterial Proteins; D002352:Carrier Proteins; D002939:Ciprofloxacin; D027081:DNA Gyrase; D027101:DNA Topoisomerase IV; D004250:DNA Topoisomerases, Type II; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D000077422:Enrofloxacin; D029968:Escherichia coli Proteins; D024841:Fluoroquinolones; D008074:Lipoproteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D009268:Nalidixic Acid; D011807:Quinolizines; D015363:Quinolones; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; C083497:AcrA protein, E coli; C083496:AcrB protein, E coli; C012976:flumequine