Antiplatelet and anticancer isothiocyanates in Japanese domestic horseradish, wasabi.
6-Methylsulfinylhexyl isothiocyanate (MS-ITC) was isolated from wasabi (Wasabia japonica, Japanese domestic horseradish) as a potential inhibitor of human platelet aggregation in vitro through our extensive screening of vegetables and fruits. In the course of another screening for the induction of glutathione S-transferase (GST) activity in RL34 cells. MS-ITC was inadvertently isolated from wasabi as a potential inducer of GST. MS-ITC administered to rats or mice also showed both activities in vivo. As a result from elucidation of the platelet aggregation inhibition and the GST induction mechanisms of MS-ITC, the isothiocyanate moiety of MS-ITC plays an important role for antiplatelet and anticancer activities because of its highly reactivity with sulfhydryl (-SH) groups in biomolecules (GSH, cysteine residue in a certain protein, etc.).
Laboratory of Food and Biodynamics, Nagoya University Graduate School of Bioagricultural Sciences, Japan. monyan@cc.ocha.ac.jp
Y
Y
Morimitsu
K
K
Hayashi
Y
Y
Nakagawa
F
F
Horio
K
K
Uchida
T
T
Osawa
D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D019607:Brassicaceae / Q000737:chemistry*; D002460:Cell Line; D005638:Fruit / Q000737:chemistry*; D005982:Glutathione Transferase / Q000096:biosynthesis; D006801:Humans; D017879:Isothiocyanates / Q000302:isolation & purification / Q000494:pharmacology*; D007564:Japan; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D017365:Spices / Q000032:analysis*; D014675:Vegetables / Q000737:chemistry*
C111984:6-methylsulfinylhexyl isothiocyanate; D000972:Antineoplastic Agents, Phytogenic; D017879:Isothiocyanates; D010975:Platelet Aggregation Inhibitors; D005982:Glutathione Transferase