Quantitation of porcine cytomegalovirus in pig tissues by PCR.
A quantitative-competitive PCR for the quantification of porcine cytomegalovirus (PCMV) was developed. The virus was detected in a variety of pig organs (including potential xenotransplant donations), with viral loads ranging from <10 to 97 genome copies/microg of DNA. This assay will have significant utility for studying the activation and replication of PCMV and in swine models for allo- and xenotransplantation.
Fryer
J F
JF
Department of Virology, Royal Free and University College Medical School of UCL, Rowland Hill Street, London NW3 2PF, United Kingdom.
Griffiths
P D
PD
Fishman
J A
JA
Emery
V C
VC
Clark
D A
DA
D000818:Animals; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000662:veterinary* / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000821:virology*; D014183:Transplantation, Heterologous
D004279:DNA, Viral