The effect of attenuated varicella-zoster virus on replication of HBV.
OBJECTIVE
To observe the effect of attenuated varicella-zoster virus (VZV) on replication of HBV in vitro and vivo.


METHODS
The attenuated VZV were inoculated into the ducks infected with DHBV and into the cultural HepG(2) 2.2.15 cells, respectively. DHBV DNA, HBsAg and HBeAg were detected from the duck serum and cell cultural fluid by dot-blot hybridization and EIA.


RESULTS
The decline of DHBV DNA in duck serum was found in two dosage groups. Compared with pre-treatment, the level of AD value in 200 pfu/kg group was significantly decreased from 1.17+/-0.29 to 0.59+/-0.45 (10 day after VZV treatment) and 0.21+/-0.21 (5 day after withdrawal of VZV)(t =3.51, 7.54, P<0.001). The level in 400 pfu/kg group was from 0.70+/-0.25 to 0.32+/-0.17 on the 5th day of withdrawal of VZV (t =3.58, P<0.01), respectively after treatment. The inhibitory rates of HBeAg and HBsAg were 61% and 33%, respectively. The effect on HBeAg seems to be more obvious than on HBsAg.


CONCLUSIONS
Attenuated VZV could significantly decrease serum DHBV DNA in DHBV-infected ducks and directly inhibit the HBeAg and HBsAg secreted by 2.2.15 cells, suggesting that VZV seems to interfere or inhibit the replication of DHBV.
Guangzhou Eighth People's Hospital, Guangzhou 510060, China.
Z
Z
Yang
X
X
Deng
F
F
Zhang
W
W
Wu
C
C
Lei
Y
Y
Zhu
D000818:Animals; D004279:DNA, Viral / Q000097:blood; D004372:Ducks; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis; D015611:Hepatitis B Virus, Duck / Q000502:physiology*; D006513:Hepatitis B e Antigens / Q000096:biosynthesis; D014645:Herpesvirus 3, Human / Q000502:physiology*; D008297:Male; D014762:Viral Interference; D014779:Virus Replication
D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens