A phase I study of RSR13, a radiation-enhancing hemoglobin modifier: tolerance of repeated intravenous doses and correlation of pharmacokinetics with pharmacodynamics.
OBJECTIVE
Preclinical studies indicate that RSR13 oxygenates and radiosensitizes hypoxic solid tumors by decreasing the oxygen (O(2))-binding affinity of hemoglobin (Hb). A Phase I open-label, multicenter dose and frequency escalation study was conducted to assess the safety, tolerance, pharmacokinetics, and pharmacodynamic effect of daily RSR13 administration to cancer patients receiving concurrent palliative radiotherapy (RT).


METHODS
Eligibility criteria included the following: ECOG performance status < or =2; resting and exercise arterial oxygen saturation (SaO(2)) > or =90%; an indication for palliative RT, 20-40 Gy in 10-15 fractions. RSR13 was administered i.v. via central vein over 60 min immediately before RT. Patients received supplemental O(2) via nasal cannula at 4 L/min during RSR13 infusion and RT. Plasma, red blood cell (RBC), and urine RSR13 concentrations were assayed. The pharmacodynamic effect of RSR13 on Hb-O(2) binding affinity was quantified by multipoint tonometry and expressed as an increase in p50, defined as the partial pressure of O(2) that results in 50% SaO(2). The RSR13 dose in the first cohort was 75 mg/kg once a week for two doses; successive cohorts received higher, more frequent doses up to 100 mg/kg/day for 10 days during RT.


RESULTS
Twenty patients were enrolled in the study. Repeated daily doses of RSR13 were generally well tolerated. Two adverse events of note occurred: (1) A patient with pre-existing restrictive lung disease had transient persistent hypoxemia after the sixth RSR13 dose; (2) a patient with a recurrent glioma receiving high-dose corticosteroids had edema after the seventh RSR13 dose, likely due to the daily high-volume fluid infusions. Both patients recovered to baseline status with conservative management. Maximum pharmacodynamic effect occurred at the end of RSR13 infusion and was proportional to the RBC RSR13 concentration. After an RSR13 dose of 100 mg/kg, the peak increase in p50 averaged 8.1 mm Hg, consistent with the targeted physiologic effect, and then diminished with a half-life of approximately 5 h.


CONCLUSIONS
RSR13 was well tolerated in daily doses up to 100 mg/kg administered for 10 days during RT. The combined administration of RSR13 with 4 L/min supplemental O(2) yielded pharmacodynamic conditions in which hypoxic tumor radiosensitization can occur. Ongoing Phase II and Phase III studies are evaluating the combination of RT and RSR13 for selected indications, including primary brain tumors, brain metastases, and non-small-cell lung cancer.
Kavanagh
B D
BD
Department of Radiation Oncology, Medical College of Virginia of Virginia Commonwealth University, Richmond, VA 23298-0058, USA. bkavanag@hsc.vcu.edu
Khandelwal
S R
SR
Schmidt-Ullrich
R K
RK
Roberts
J D
JD
Shaw
E G
EG
Pearlman
A D
AD
Venitz
J
J
Dusenbery
K E
KE
Abraham
D J
DJ
Gerber
M J
MJ
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000814:Aniline Compounds; D015687:Cell Hypoxia / Q000187:drug effects* / Q000528:radiation effects; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D006441:Hemoglobin A / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000097:blood / Q000532:radiotherapy*; D010100:Oxygen / Q000097:blood*; D010313:Partial Pressure; D011422:Propionates / Q000008:administration & dosage / Q000009:adverse effects* / Q000493:pharmacokinetics; D011838:Radiation-Sensitizing Agents / Q000008:administration & dosage / Q000009:adverse effects* / Q000493:pharmacokinetics; D011879:Radiotherapy Dosage
D000814:Aniline Compounds; D011422:Propionates; D011838:Radiation-Sensitizing Agents; D006441:Hemoglobin A; C067396:efaproxiral; D010100:Oxygen