Cytokines and vascular cell adhesion molecule-1 in the blood of patients undergoing HPC mobilization.
BACKGROUND
The mechanism of HPC mobilization in humans is unclear. In this study, the relationship between PBPC mobilization and blood levels of G-CSF, endogenous cytokines (IL-8, SCF, thrombopoietin [TPO]), and the vascular cell adhesion molecule-1 (VCAM-1) was analyzed in patients with malignancy who were undergoing a PBPC mobilization regimen.


METHODS
Fifty-four patients with multiple myeloma (MM) and 29 with breast cancer (BC) underwent a mobilization regimen combining conventional chemotherapy and G-CSF up to the last day of PBPC collection. The CD34+ cell count was determined on each day when leukapheresis was scheduled. Venous blood samples (n = 117) were drawn before apheresis for CD34+ cell count (flow cytometry) and cytokine (G-CSF, IL-8, SCF, TPO) and VCAM-1 measurements (ELISA).


RESULTS
In multiple regression analysis, SCF was a significant determinant of CD34+ cell levels in BC patients (R = 0.50, p = 0.03) and of VCAM-1 levels in MM patients (R = 0.32, p = 0.02). SCF was negatively correlated with CD34+ cell count in patients with BC. SCF and VCAM-1 blood levels were correlated in MM and BC patients.


CONCLUSIONS
SCF and VCAM-1 could play a role in PBPC mobilization in patients and could be useful measures by which to study patients undergoing a mobilization regimen.
Cell Therapy Unit and the Departments of Hematology and Oncology, Saint-Louis Hospital, Paris. France. dosquet@chu-stlouis.fr
C
C
Dosquet
Y
Y
Chen
J
J
Makke
J M
JM
Miclea
M C
MC
Coudert
J P
JP
Marolleau
J P
JP
Fermand
P
P
Cottu
J P
JP
Lotz
M
M
Benbunan
D000328:Adult; D018952:Antigens, CD34 / Q000097:blood; D001943:Breast Neoplasms / Q000097:blood / Q000276:immunology; D016207:Cytokines / Q000097:blood*; D005260:Female; D019650:Hematopoietic Stem Cell Mobilization; D006412:Hematopoietic Stem Cells / Q000276:immunology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000097:blood / Q000276:immunology; D019010:Vascular Cell Adhesion Molecule-1 / Q000097:blood*
D018952:Antigens, CD34; D016207:Cytokines; D019010:Vascular Cell Adhesion Molecule-1