[Comparative studies of mitochondrial DNA of T-type cytoplasmic male sterile lines and their maintainers in wheat].
mtDNAs of T type wheat cytoplasmic male sterile lines 75-3369A and their maintainers 75-3369B were isolated and digested completely with restriction endonuclease BamH I, EcoR I, Hind III. The mitochondrial genomic difference between CMS line and maintainers were revealed by Southern hybridization with probes of atp6 from Oenothera and nad3/rps12, cox I from wheat mitochondria. Southern hybridization showed the differences between these two lines. Discussion was made on the change of the DNA composition and the male sterile cytoplasm.
Qiu
Y G
YG
Hebei Normal University, Life and Science Department, Shijiazhuang 050016, China.
Shen
Y Z
YZ
Huang
Z J
ZJ
Zhao
B C
BC
He
C F
CF
Bi
C L
CL
D015139:Blotting, Southern; D004272:DNA, Mitochondrial / Q000235:genetics*; D005298:Fertility; D014908:Triticum / Q000235:genetics*
D004272:DNA, Mitochondrial