Involvement of mitochondrial ferredoxin and Cox15p in hydroxylation of heme O.
Cox15p is essential for the biogenesis of cytochrome oxidase [Glerum et al., J. Biol. Chem. 272 (1997) 19088-19094]. We show here that cox15 mutants are blocked in heme A but not heme O biosynthesis. In Schizosaccharomyces pombe COX15 is fused to YAH1, the yeast gene for mitochondrial ferredoxin (adrenodoxin). A fusion of Cox15p and Yah1p in Saccharomyces cerevisiae rescued both cox15 and yah1 null mutants. This suggests that Yah1p functions in concert with Cox15p. We propose that Cox15p functions together with Yah1p and its putative reductase (Arh1p) in the hydroxylation of heme O.
Barros
M H
MH
Department of Biological Sciences, Columbia University, New York, NY 10027, USA.
Carlson
C G
CG
Glerum
D M
DM
Tzagoloff
A
A
D000325:Adrenodoxin; D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D003573:Cytochrome b Group / Q000737:chemistry; D005288:Ferredoxins / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D006418:Heme / Q000031:analogs & derivatives / Q000378:metabolism*; D006900:Hydroxylation; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; C107633:COX15 protein, S cerevisiae; C090098:CtaA protein, bacteria; D003573:Cytochrome b Group; D005288:Ferredoxins; D005656:Fungal Proteins; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; C120375:YAH1 protein, S cerevisiae; D000325:Adrenodoxin; C069808:heme O; C027728:heme a; D006418:Heme; D003577:Cytochrome P-450 Enzyme System