Set of vectors for the expression of histidine-tagged proteins in vaccinia virus recombinants.
Vaccinia virus expression vectors are widely used to direct the expression of proteins in eukaryotic cells. Here, we describe a new set of plasmid vectors designed for the expression of histidine-tagged proteins in the vaccinia system. To facilitate the rapid isolation of virus recombinants, the plasmids contain a viral gene (F13L) that serves as an efficient selection marker based on virus plaque phenotype. Histidine codons and restriction sites derived from pET-16b bacterial expression plasmid were included, thus facilitating the transfer of genes between E. coli and vaccinia expression plasmids. Plasmids in which the gene is placed downstream of either a strong vaccinia virus or a T7 promoter were constructed, allowing for constitutive or conditional expression, respectively, of the foreign protein.
Galindo
I
I
Departamento de Mejora genética y biotecnología, INIA, Ctra. La Coruña km 7, E-28040 Madrid, Spain.
Lorenzo
M M
MM
Blasco
R
R
D000595:Amino Acid Sequence; D001483:Base Sequence; D005822:Genetic Vectors; D006639:Histidine / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis*; D014616:Vaccinia virus / Q000235:genetics*
D011994:Recombinant Proteins; D006639:Histidine