Correlation between the spermatozoal characteristics and sperm penetration distance in polyacrylamide gel and bovine cervical mucus.
Correlation between the spermatozoal characteristics and the sperm penetration distance in polyacrylamide gel was assessed, utilizing frozen thawed semen samples obtained from 6 bulls, and it was compared with the correlation between sperm penetration in bovine cervical mucus and spermatozoal characteristics. In vitro sperm penetration tests were performed with mucus and gel. The sperm penetration in gel and mucus was significantly and positively correlated with post-thaw motility (r=0.81; r=0.89:P<0.01) and acrosome integrity (r=0.88; r=0.94:P<0.01). A significant negative correlation with abnormal spermatozoa (r=-0.84;r=0.83:P<0.01) was observed. Both sperm concentration and post-thaw live spermatozoa were not significantly correlated. A significant multiple regression between sperm penetration and the spermatozoal characteristics both in gel (R2=0.87; F=40.27; P<0.01) and mucus (R2=0.91; F=60.48; P<0.01) was observed. The major spermatozoal characteristics determining the capacity of spermatozoa to penetrate gel were post-thaw motility, percentage of abnormal spermatozoa and acrosome integrity. The acrosome integrity has a more significant contribution. The correlation established with sperm penetration in gel was very similar to that of sperm penetration in mucus. The utility of gel as a mucus substitute in in vitro sperm penetration tests was discussed.
Department of Obstetrics and Gynaecology, Madras Veterinary College, Chennai, India.
R
R
Anilkumar
T G
TG
Devanathan
S R
SR
Pattabiraman
M J
MJ
Edwin
D000177:Acrosome / Q000502:physiology; D000180:Acrylic Resins; D000818:Animals; D001399:Azure Stains / Q000737:chemistry; D002417:Cattle / Q000502:physiology*; D002582:Cervix Mucus / Q000502:physiology*; D005260:Female; D008297:Male; D008858:Microscopy, Phase-Contrast / Q000662:veterinary; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary; D013081:Sperm Motility / Q000502:physiology; D013084:Sperm-Ovum Interactions / Q000502:physiology*; D013094:Spermatozoa / Q000737:chemistry / Q000502:physiology*
D000180:Acrylic Resins; D001399:Azure Stains; C016680:polyacrylamide gels