Redox control of Epstein-Barr virus replication by human thioredoxin/ATL-derived factor: differential regulation of lytic and latent infection.
Human thioredoxin (hTRX)/adult T-cell leukemia (ATL)-derived factor (ADF) was originally reported as an interleukin-2 (IL-2) receptor-alpha-inducing factor produced by human T-cell lymphotropic virus-1-positive (HTLV-1+) cell lines. Growing evidence indicates that hTRX/ADF plays important roles in cellular responses against oxidative stress and is involved in a variety of cellular functions. A high level of hTRX/ADF expression is also observed in other human virus-infected cell lines including those of Epstein-Barr virus (EBV) and human papillomavirus. In this report, we analyzed the effect of hTRX/ADF on lytic amplification and persistent replication of EBV as a model for lytic versus latent phase of viral replication in host cells. Addition of hTRX/ADF clearly suppressed lytic replication of EBV in Raji cells and B95-8 cells induced to the lytic phase of 12-O-tetradecanoylphorbol-13-acetate (TPA), and it prevented the death of these cells evoked by the lytic induction. In contrast, hTRX/ADF did not have any effect on persistent replication in the latent phase. These data indicated that hTRX/ADF prevents EBV-transformed cells from proceeding into the lytic phase and regulates cohabitation of EBV and its host cells.
Sono
H
H
Department of Biological Responses, Institute for Virus Research, Kyoto University, Kyoto, Japan.
Teshigawara
K
K
Sasada
T
T
Takagi
Y
Y
Nishiyama
A
A
Ohkubo
Y
Y
Maeda
Y
Y
Tatsumi
E
E
Kanamaru
A
A
Yodoi
J
J
D002273:Carcinogens / Q000494:pharmacology; D016923:Cell Death / Q000187:drug effects; D002461:Cell Line, Transformed / Q000821:virology; D002472:Cell Transformation, Viral / Q000235:genetics; D016207:Cytokines / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D004261:DNA Replication / Q000235:genetics; D004279:DNA, Viral / Q000037:antagonists & inhibitors / Q000235:genetics; D005784:Gene Amplification; D015967:Gene Expression Regulation, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity* / Q000502:physiology; D006801:Humans; D007244:Infectious Mononucleosis / Q000235:genetics / Q000378:metabolism* / Q000821:virology*; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000821:virology; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D010084:Oxidation-Reduction; D013489:Suppression, Genetic; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013879:Thioredoxins / Q000494:pharmacology*; D014779:Virus Replication / Q000235:genetics
D002273:Carcinogens; D016207:Cytokines; D004279:DNA, Viral; D009363:Neoplasm Proteins; C055533:adult T cell leukemia-derived factor; D013879:Thioredoxins; D013755:Tetradecanoylphorbol Acetate