Sporocyst size of isolates of Sarcocystis shed by the Virginia opossum (Didelphis virginiana).
The Virginia opossum (Didelphis virginiana) is a definitive host for multiple Sarcocystis species including Sarcocystis neurona, one of the causative agents of equine protozoal myeloencephalitis (EPM), a severe, neuromuscular disease of horses. Size and morphologic characteristics of isolates of Sarcocystis shed by the opossum were examined to determine if differences were useful in discriminating between the isolates and/or species. Collections of sporocysts from 17 opossums were molecularly characterized and measured using an ocular micrometer. The mean sporocyst size of isolates of S. neurona was 10.7 microm x 7.0 microm, Sarcocystis falcatula 11.0 microm x 7.1 microm, Sarcocystis speeri 12.2 microm x 8.8 microm, 1085-like isolate 10.9 microm x 6.8 microm, and 3344-like isolate 19.4 microm x 10.5 microm. The length and width of S. speeri were statistically different (p < 0.05) from the sporocysts of other types. The length of S. neurona and S. falcatula sporocysts were statistically different (p < 0.05) from each other and the width of S. falcatula and 1085 differed (p < 0.05). The fifth sporocyst type (3344) was observed, but due to pronounced morphological characteristics, statistical analysis was not performed. There was no consistent difference between the taxa based on internal structure of the sporocyst.
Department of Pathobiology, College of Veterinary Medicine, University of Florida, Gainesville 32610-0880, USA. cheadlem@mailvetmed.ufl.edu
M A
MA
Cheadle
J B
JB
Dame
E C
EC
Greiner
D000818:Animals; D016054:DNA, Protozoan / Q000737:chemistry; D005243:Feces / Q000469:parasitology; D005819:Genetic Markers; D007422:Intestines / Q000469:parasitology; D008853:Microscopy / Q000379:methods / Q000662:veterinary*; D009893:Opossums / Q000469:parasitology*; D012522:Sarcocystis / Q000145:classification / Q000302:isolation & purification* / Q000648:ultrastructure; D014768:Virginia
D016054:DNA, Protozoan; D005819:Genetic Markers