The stability between two HIV-1 RNA measurements one year apart and the relationship with HIV subtype in rural Uganda.
We compared HIV-1 RNA levels using the nucleic acid sequenced based amplification (NASBA) test kit in 2 samples taken one year apart from participants infected with env subtype A or D in a population-based cohort in Uganda. Fifty participants were infected with subtype A and 70 with subtype D. HIV-1 RNA levels were significantly higher in subtype D unadjusted (P=0.001), and after adjusting for age, gender, and CD4 count (P<0.001). Eighty-six participants had HIV-1 RNA measurements in both years and 67 (78%) were within one log10 of their result a year before. There was no relationship between the difference in log viral load and proportion of CD4 change. Individuals infected with subtype D had a higher average increase in viral load and this was statistically significant if adjusted for baseline levels and CD4 count (P=0.015).
Medical Research Council Programme on AIDS, Uganda Virus Research Institute, Entebbe. mrc@starcom.co.ug
D
D
Morgan
P
P
Kaleebu
J
J
Whitworth
D
D
Yirrell
A
A
Rutebemberwa
R
R
Shier
B
B
Sebabi
J
J
Gilmour
H
H
Eotu
N
N
Omoding
F
F
Gotch
D000293:Adolescent; D000328:Adult; D018791:CD4 Lymphocyte Count; D005260:Female; D005784:Gene Amplification; D015658:HIV Infections / Q000453:epidemiology / Q000821:virology*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D021141:Nucleic Acid Amplification Techniques; D012367:RNA, Viral / Q000032:analysis*; D011933:Reagent Kits, Diagnostic; D012424:Rural Population; D014454:Uganda / Q000453:epidemiology; D019562:Viral Load
D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic