Rapid PCR detection of Salmonella in horse faecal samples.
A rapid polymerase chain reaction (PCR) assay was developed for detecting Salmonella in faeces of horses and assessed on samples from horses admitted to a veterinary hospital. Direct detection was achieved by amplification of part of ompC after extraction of DNA from faeces using a spin column method to reduce the amount of inhibitory substances in samples. An internal positive control was included to detect false negative results. While the sensitivity of the PCR assay was less than culture when assessed on faeces inoculated with Salmonella, its sensitivity on faecal samples obtained from horses was much greater than culture. Salmonella DNA was detected in 40% of faecal samples using the PCR assay while Salmonella were cultured from only 2% of the samples. The PCR assay has potential for use in either routine diagnosis or for detection of the carrier status in animals.
Faculty of Veterinary Science, The University of Melbourne, Vic. 3010, Parkville, Australia.
P
P
Amavisit
G F
GF
Browning
D
D
Lightfoot
S
S
Church
G A
GA
Anderson
K G
KG
Whithear
P F
PF
Markham
D000818:Animals; D015169:Colony Count, Microbial / Q000662:veterinary; D005243:Feces / Q000382:microbiology*; D006736:Horses / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D018272:Porins / Q000235:genetics; D012475:Salmonella / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
C113724:OmpC protein; D018272:Porins