In vivo titration of mitomycin C action by four Escherichia coli genomic regions on multicopy plasmids.
Mitomycin C (MMC), a DNA-damaging agent, is a potent inducer of the bacterial SOS response; surprisingly, it has not been used to select resistant mutants from wild-type Escherichia coli. MMC resistance is caused by the presence of any of four distinct E. coli genes (mdfA, gyrl, rob, and sdiA) on high-copy-number vectors. mdfA encodes a membrane efflux pump whose overexpression results in broad-spectrum chemical resistance. The gyrI (also called sbmC) gene product inhibits DNA gyrase activity in vitro, while the rob protein appears to function in transcriptional activation of efflux pumps. SdiA is a transcriptional activator of ftsQAZ genes involved in cell division.
Biochemical Science and Engineering, Central Research and Development, DuPont Company, Wilmington, Delaware 19880-0173, USA.
Y
Y
Wei
A C
AC
Vollmer
R A
RA
LaRossa
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000502:physiology; D004249:DNA Damage; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics; D029968:Escherichia coli Proteins; D018628:Gene Dosage; D016680:Genome, Bacterial; D016685:Mitomycin / Q000494:pharmacology*; D010957:Plasmids; D012697:Serine Endopeptidases / Q000502:physiology; D015534:Trans-Activators / Q000235:genetics; D000099160:LexA Repressor Protein
D000903:Antibiotics, Antineoplastic; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D016685:Mitomycin; D012697:Serine Endopeptidases; D015534:Trans-Activators; D000099160:LexA Repressor Protein; C097686:SdiA protein, bacteria; C490806:sdiA protein, E coli