Epidemiological typing of Candida albicans from bloodstream infections by restriction enzyme analysis.
Candida albicans blood culture isolates (n = 142) from 130 patients treated in 7 acute care hospitals in Southern Finland between 1986 and 1995 were typed by restriction enzyme analysis (REA) with EcoRI and MspI. REA revealed 118 distinct DNA types: 13 were detected in > 1 patient and 105 DNA types were unique. Patients with identical DNA types were not temporally or geographically clustered. Among 11 patients with more than 1 C. albicans isolate during 1 episode of fungemia the DNA type remained the same, except in 1 patient. These results indicate that REA is a powerful tool for the epidemiological typing of C. albicans infections.
Helsinki University Hospital, Internal Medicine Clinic, Finland.
E
E
Poikonen
J
J
Vuopio-Varkila
S S
SS
Kaukoranta-Tolvanen
A
A
Sivonen
E
E
Siren
P
P
Ruutu
D002176:Candida albicans / Q000145:classification / Q000235:genetics*; D002177:Candidiasis / Q000382:microbiology*; D004271:DNA, Fungal / Q000032:analysis*; D016469:Fungemia / Q000382:microbiology*; D006801:Humans; D000091402:Prohibitins; D015183:Restriction Mapping
D004271:DNA, Fungal; C000717331:PHB2 protein, human; D000091402:Prohibitins