[Nucleoprotein complexes of yeast and their biological effect on T-lymphocytes].
Complexes of nucleic acids and acid nuclear proteins that are active toward human T-lymphocytes were isolated from cells of bakers' yeast Saccharomyces cerevisiae. The conditions of isolation of nucleoprotein complexes by acid extraction followed by microfiltration for concentration of macromolecular components were optimized. Gel filtration and electrophoresis were used to study the composition and molecular weights of components of the preparations obtained. It was shown that nucleoprotein complex had a molecular weight of 1430 kDa. However, only one zone was determined by electrophoresis of the protein component with a molecular weight of 30 kDa.
Sinitskaia
N S
NS
Institute of Macromolecular Compounds, Russian Academy of Sciences, St. Petersburg, 199053 Russia.
Shataeva
L K
LK
Potekhina
T S
TS
Kas'ianov
A D
AD
D005656:Fungal Proteins / Q000276:immunology / Q000494:pharmacology*; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects; D009698:Nucleoproteins / Q000276:immunology / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology
D005656:Fungal Proteins; D009698:Nucleoproteins