Determination of morpholine in air by derivatisation with 1-naphthylisothiocyanate and HPLC analysis.
A method for the determination of morpholine in air was developed. Samples were collected with adsorbent tubes containing XAD-2 resin coated with 1-naphthylisothiocyanate (NIT). The thiourea derivative formed was subsequently desorbed with acetonitrile and analysed by HPLC with UV detection. The recovery after gas phase spiking with morpholine (2.2-1570 micrograms) was 91% (86-100%) with a relative standard deviation of 5.5%. No effect on recovery from relative humidity or amount of morpholine was seen. The lowest level tested corresponded to 7 mg m-3 (1/10 threshold limit value) for a 15 min sampling period with a sampling rate of 20 ml min-1. Exposed NIT-coated XAD-2 tubes were stable at room temperature for at least 2 weeks.
Lindahl
R
R
National Institute for Working Life, POB 7654, S-907 13 Umeå, Sweden. lindahl@niwl.se
Wästerby
A
A
Levin
J O
JO
D015058:1-Naphthylisothiocyanate; D000395:Air Pollutants, Occupational / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D009025:Morpholines / Q000032:analysis*
D000395:Air Pollutants, Occupational; D009025:Morpholines; D015058:1-Naphthylisothiocyanate; C037574:morpholine