HLA-associated cellular response to GAD in type 2 diabetes with antibodies to GAD.
Proliferative response of peripheral blood mononuclear cells (PBMC) to glutamic acid decarboxylase (GAD), which has been reported in patients with type 1 diabetes, was measured in type 2 diabetes, especially in patients with antibodies to GAD initially diagnosed as having type 2 diabetes (anti-GAD+ type 2 diabetes). We studied 12 patients with type 1 diabetes, 22 with anti-GAD+ type 2 diabetes, 31 with type 2 diabetes who were negative for anti-GAD (anti-GAD+ type 2 diabetes), and 30 healthy control subjects for cellular responses in vitro to GAD. The mean stimulation index (SI) in response to GAD was significantly higher in type 1 diabetes than in anti-GAD+ type 2 diabetes or healthy controls (1.47+/-0.35 vs. 1.11+/-0.35, P<0.05, and 1.06+/-0.07, P<0.05, respectively). The mean
The First Department of Internal Medicine, Kyoto Prefectural University of Medicine, Japan.
M
M
Fukui
N
N
Nakamura
K
K
Nakano
S
S
Kajiyama
S
S
Matsuo
H
H
Obayashi
M
M
Ohta
M
M
Shigeta
H
H
Shigeta
Y
Y
Kitagawa
M
M
Kondo
D000328:Adult; D000368:Aged; D000483:Alleles; D001323:Autoantibodies / Q000097:blood*; D001324:Autoantigens / Q000276:immunology; D002455:Cell Division / Q000187:drug effects; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics / Q000276:immunology; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics* / Q000276:immunology*; D005260:Female; D020022:Genetic Predisposition to Disease; D005968:Glutamate Decarboxylase / Q000276:immunology*; D006680:HLA Antigens / Q000235:genetics*; D006684:HLA-DR Antigens / Q000235:genetics; D059811:HLA-DRB1 Chains; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction
D001323:Autoantibodies; D001324:Autoantigens; D006680:HLA Antigens; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D007328:Insulin; D005968:Glutamate Decarboxylase