Monitoring the production yields of vincristine and vinblastine in Catharanthus roseus from somatic embryogenesis. Semiquantitative determination by flow-injection electrospray ionization mass spectrometry.
A semiquantitative determination of two bis-indole antitumor alkaloids, vincristine and vinblastine, has been performed by flow-injection electrospray ionization mass spectrometric analysis of the extracts of Catharanthus roseus. Leaves and flowers of two different phenotypes (pink flower and white flower) obtained from somatic embryogenesis were thus examined and compared with the field-grown mother plant. Different amounts of vincristine and vinblastine were detected depending on the examined samples.
Favretto
D
D
Centro di Studio sulla Chimica e Tecnologia dei Composti Organometallici degli Elementi di Transizione, Via Marzolo 9, I-35100 Padua, Italy. favretto@pdadr1.pd.cnr.it
Piovan
A
A
Filippini
R
R
Caniato
R
R
D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis* / Q000096:biosynthesis; D002138:Calibration; D017022:Flow Injection Analysis; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry* / Q000196:embryology / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D014747:Vinblastine / Q000032:analysis* / Q000096:biosynthesis; D014750:Vincristine / Q000032:analysis* / Q000096:biosynthesis
D000972:Antineoplastic Agents, Phytogenic; D014750:Vincristine; D014747:Vinblastine