Evaluation of two concentration methods for detecting Giardia and Cryptosporidium in water.
The cartridge filtration method and membrane filtration method based on the fluorescent antibody procedure were evaluated for their recovery efficiencies and detection limits of Giardia and Cryptosporidium. We assessed the performances of the two concentration methods for water samples collected from Taiwan water treatment plants. The membrane filtration method was characterized by higher recovery rate and detection limit comparing with the cartridge filtration method. The occurrences of both parasites, and the relationships of parasite concentrations with indicator microorganisms show inconsistency between the two methods. It was discovered that water turbidity reduced the recovery efficiencies, and raised the detection limits for both parasites regardless of the method used.
Hsu
B M
BM
Institute of Environmental Engineering, National Chiao Tung University, 75, Po-Ai Street, Hsinchu, Taiwan, ROC.
Huang
C
C
Hsu
Y F
YF
Jiang
G Y
GY
Hsu
C L
CL
D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis; D003458:Cryptosporidium / Q000302:isolation & purification*; D005374:Filtration / Q000379:methods; D005455:Fluorescent Antibody Technique; D005872:Giardia / Q000302:isolation & purification*; D005873:Giardiasis / Q000517:prevention & control; D009391:Nephelometry and Turbidimetry; D018709:Statistics, Nonparametric; D013624:Taiwan; D014867:Water / Q000469:parasitology*; D018508:Water Purification / Q000379:methods*
D000913:Antibodies, Protozoan; D014867:Water