Characterization of the X-linked murine centrin Cetn2 gene.
A multi-gene family (Cetn1, Cetn2, and Cetn3) encodes the calcium-binding protein, centrin, in the mouse. This work characterizes the Cetn2 gene. Structurally, Cetn2 consists of five exons and four introns, and contains a classical TATA-less promoter. Cetn2 has two alternate transcription start sites, and a single length 3' untranslated region. Fluorescence in situ hybridization demonstrates that Cetn2 is an X-linked gene whose alleles replicate asynchronously during S-phase. Cetn2 encodes a 172 amino acid protein, with a predicted molecular mass of 19,795 Da (pI=4.71), that contains all of the defining characteristics of centrin. Northern blot analysis indicates that Cetn2 is ubiquitously expressed in the tissues of adult mice. RT-PCR shows that Cetn2 and Cetn3, but not Cetn1, are expressed in NIH 3T3 cells. Immunofluorescence microscopy demonstrates that mouse centrin 2 protein localizes to the region immediately surrounding the centrioles in the centrosome of NIH 3T3 cells.
Hart
P E
PE
Tumor Biology Program, Mayo Foundation, Rochester, MN 55905, USA.
Poynter
G M
GM
Whitehead
C M
CM
Orth
J D
JD
Glantz
J N
JN
Busby
R C
RC
Barrett
S L
SL
Salisbury
J L
JL
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000235:genetics*; D002868:Chromosomal Proteins, Non-Histone; D004247:DNA / Q000737:chemistry / Q000235:genetics; D005091:Exons; D005260:Female; D005796:Genes / Q000235:genetics*; D008040:Genetic Linkage; D017404:In Situ Hybridization, Fluorescence; D007438:Introns; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014960:X Chromosome / Q000235:genetics*
D002135:Calcium-Binding Proteins; C424436:Cetn2 protein, mouse; D002868:Chromosomal Proteins, Non-Histone; D012333:RNA, Messenger; C056608:caltractin; D004247:DNA