An RNA ligase essential for RNA editing and survival of the bloodstream form of Trypanosoma brucei.
RNA editing in trypanosomes occurs by a series of enzymatic steps that are catalyzed by a macromolecular complex. The TbMP52 protein is shown to be a component of this complex, to have RNA ligase activity, and to be one of two adenylatable proteins in the complex. Regulated repression of TbMP52 blocks editing, which shows that it is a functional component of the editing complex. This repression is lethal in bloodforms of the parasite, indicating that editing is essential in the mammalian stage of the life cycle. The editing complex, which is present in all kinetoplastid parasites, may thus be a chemotherapeutic target.
Seattle Biomedical Research Institute, Seattle, WA 98109, USA.
A
A
Schnaufer
A K
AK
Panigrahi
B
B
Panicucci
R P
RP
Igo
E
E
Wirtz
R
R
Salavati
K
K
Stuart
D000595:Amino Acid Sequence; D000818:Animals; D015536:Down-Regulation; D018390:Gene Targeting; D017125:Genes, Protozoan; D008025:Ligases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D018512:Parasitemia / Q000469:parasitology; D019761:Phosphorus-Oxygen Lyases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017393:RNA Editing; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016053:RNA, Protozoan / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D014162:Transfection; D014346:Trypanosoma brucei brucei / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D014353:Trypanosomiasis, African / Q000188:drug therapy / Q000469:parasitology
D015800:Protozoan Proteins; D012333:RNA, Messenger; D016053:RNA, Protozoan; C418993:TbMP52 protein, Trypanosoma brucei; D019761:Phosphorus-Oxygen Lyases; D008025:Ligases; C418994:MP48 protein, Trypanosoma brucei