Growth regulation of ovarian cancer cell line HO-8910 by transforming growth factor beta 1 in vitro.
OBJECTIVE
To further understand the role of growth regulation of human ovarian cancer cells by transforming growth factor (TGF) beta 1.


METHODS
The cell proliferation, cAMP synthesis, gene expression, and induction of programmed cell death (PCD) in human epithelial ovarian cancer cell line HO-8910 cells exposed to TGF beta 1 in vitro were studied.


RESULTS
TGF beta 1 inhibited cell growth and DNA synthesis, and induced G0/G1 arrest in cell cycle. It could also trigger PCD in cells. This induction of PCD may occur within G0/G1 phase. Meanwhile, the assay also showed that TGF beta 1 could inhibit the mRNA expression of c-myc, EGFR and TGF beta 1 genes in cells.


CONCLUSIONS
TGF beta 1 can not only act as an autocrine to inhibit cell proliferation, but also trigger PCD in HO-8910 cells. These functions may be fulfilled through some specific signal transduction pathways.
Cancer Research Institute, Zhejiang Medical University, Hangzhou 310006, China.
X
X
Chen
S
S
Zheng
Y
Y
Gao
H
H
Dai
H
H
Mou
H
H
Yang
D017209:Apoptosis / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D018283:Cystadenocarcinoma, Papillary / Q000473:pathology*; D005260:Female; D006801:Humans; D010051:Ovarian Neoplasms / Q000473:pathology*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D016212:Transforming Growth Factor beta