Platelet serotonin transporters and the  transporter gene in control subjects, unipolar patients and bipolar patients.
OBJECTIVE
The purpose of the present study was to relate the number of platelet serotonin transporters in unipolar and bipolar patients and in control subjects to two polymorphisms in the serotonin transporter gene: a VNTR in intron 2 and a deletion/insertion in the promoter region.


METHODS
Density of platelet serotonin transporters was determined by radioligand binding analysis. Genotyping was performed by PCR amplification of polymorphic regions followed by size determination of the obtained fragments.


RESULTS
The control subjects and the two groups of patients were similar with respect to the genotype and allele distribution belonging to the two polymorphisms in the serotonin transporter gene for. An interaction between status (control, unipolar- or bipolar patient) and VNTR genotype regarding the number of platelet serotonin transporters was observed; unipolar patients with the genotype 12/10 had more platelet serotonin transporters than bipolar patients and controls with this genotype. No association related to the polymorphism was found in the promoter region of the serotonin transporter gene.


CONCLUSIONS
An association was observed between the polymorphism in intron 2 of the serotonin transporter gene and the number of platelet serotonin transporters. Unipolar patients with a particular genotype had more platelet serotonin transporters than the corresponding controls and bipolar patients.
Department of Psychiatry, Rigshospitalet, Copenhagen, Denmark.
E
E
Mellerup
B
B
Bennike
T
T
Bolwig
H
H
Dam
L
L
Hasholt
M B
MB
Jørgensen
P
P
Plenge
S A
SA
Sørensen
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D001692:Biological Transport / Q000502:physiology; D001714:Bipolar Disorder / Q000097:blood / Q000235:genetics; D002352:Carrier Proteins / Q000097:blood* / Q000235:genetics*; D003866:Depressive Disorder / Q000097:blood* / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics; D008875:Middle Aged; D018598:Minisatellite Repeats / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000235:genetics; D011110:Polymorphism, Genetic / Q000235:genetics; D012701:Serotonin / Q000097:blood* / Q000235:genetics*
D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D012701:Serotonin