High-performance liquid chromatographic determination of oxolinic acid and flumequine in the live fish feed artemia.
A high-performance liquid chromatography (HPLC) analytical method for the determination of oxolinic acid and flumequine in Artemia nauplii is described. The samples were extracted and cleaned up by a solid-phase extraction (SPE) procedure using SPE C18 cartridges. Oxolinic acid and flumequine were determined by reversed-phase HPLC using a mobile phase of methanol-0.1 M phosphate buffer, pH 3 (45:55, v/v) and a UV detection wavelength of 254 nm. Calibration curves were linear for oxolinic acid in the range of 0.2-50 microg/g (r2=0.9998) and for flumequine in the range of 0.3-50 microg/g (r2=0.9994). Mean recoveries amounted to 100.8% and 98.4% for oxolinic acid and flumequine, respectively. The quantification limit was 0.2 microg/g for oxolinic acid and 0.3 microg/g for flumequine. Quantitative data from an in vivo feeding study indicated excellent uptake of both drugs by Artemia nauplii.
Department of Genetics, Development and Molecular Biology, School of Biology, Faculty of Sciences, Aristotle University of Thessaloniki, Greece. touraki@bio.auth.gr
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Touraki
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Ladoukakis
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Prokopiou
D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis*; D001156:Artemia / Q000737:chemistry*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D024841:Fluoroquinolones; D010093:Oxolinic Acid / Q000032:analysis*; D011807:Quinolizines / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D011807:Quinolizines; D010093:Oxolinic Acid; C012976:flumequine