[Study on the cellular immune function and cytokines in aplastic anemia patients].
OBJECTIVE
To evaluate the effects of cellular immune function and cytokines on the pathogenesis of aplastic anemia (AA) and its clinical significance.


METHODS
T lymphocyte subsets and HLA-DR antigen expression in the peripheral blood cells were assayed, and the levels of G-CSF, IL-6, TNF alpha, IFN alpha and IL-8 in the PBMNC culture supernatants were determined in 38 AA patients and 20 normal control with APAAP and ELISA methods.


RESULTS
CD4+ cells, CD4+/CD8+ cells and G-CSF level were lower, and CD8+ cells, HLA-DR+ cells and IL-6, TNF alpha, IFN alpha and IL-8 levels were higher in AA patients than in normal controls. The level of G-CSF was positively correlated with CD4+ cells and CD4+/CD8+ cells and negatively with IFN alpha level. IL-6 level was negatively correlated with WBC count and CD4+ cells. TNF alpha level was positively correlated with CD8+ cells and negatively with CD4+/CD8+ cells. IL-8 level was positively correlated with CD8+ cells and HLA-DR+ cells.


CONCLUSIONS
The cellular immune dysfunction and cytokine aberration participate in the pathogenesis of AA.
Affiliated Hospital of Shandong Medical University, Jinan 250012.
X
X
Wang
M
M
Zhang
S
S
Song
D000328:Adult; D000741:Anemia, Aplastic / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000276:immunology*; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology; D016209:Interleukin-8 / Q000276:immunology*; D008297:Male; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D006684:HLA-DR Antigens; D016209:Interleukin-8; D016179:Granulocyte Colony-Stimulating Factor