A heart-specific CD4+ T-cell line obtained from a chronic chagasic mouse induces carditis in heart-immunized mice and rejection of normal heart transplants in the absence of Trypanosoma cruzi.
To study the role of autoreactive T cells in the pathogenesis of cardiomyopathy in Chagas' disease, we generated a cell line by repeated in vitro antigenic stimulation of purified splenic CD4+ T lymphocytes from a chronically Trypanosoma cruzi-infected mouse. Cells from this line were confirmed to be CD4+ CD8- and proliferated upon stimulation with soluble heart antigens from different animal species, as well as with T. cruzi antigen, in the presence of syngeneic feeder cells. In vitro antigen stimulation of the cell line produced a Th1 cytokine profile, with high levels of IFNgamma and IL-2 and absence of IL-4, IL-5 and IL-10. The cell line also terminated the beating of fetal heart clusters in vitro when cocultured with irradiated syngeneic normal spleen cells. In situ injection of the cell line into well established heart transplants also induced the cessation of heart beating. Finally, adoptive transfer of the cell line to heart-immunized or T. cruzi-infected BALB/c nude mice caused intense heart inflammation.
Centro de Pesquisas Gonçalo Moniz, FIOCRUZ, Bahia, Brazil. rrsantos@e-net.com.br
R
R
Ribeiro-Dos-Santos
J O
JO
Mengel
E
E
Postol
R A
RA
Soares
E
E
Ferreira-Fernandez
M B
MB
Soares
L C
LC
Pontes-De-Carvalho
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D014355:Chagas Disease / Q000276:immunology*; D002908:Chronic Disease; D018920:Coculture Techniques; D004195:Disease Models, Animal; D006084:Graft Rejection; D016027:Heart Transplantation; D007371:Interferon-gamma / Q000032:analysis; D007376:Interleukin-2 / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008819:Mice, Nude; D009205:Myocarditis / Q000276:immunology; D009206:Myocardium / Q000166:cytology / Q000276:immunology; D011817:Rabbits; D051381:Rats; D013154:Spleen / Q000276:immunology; D014349:Trypanosoma cruzi
D007376:Interleukin-2; D007371:Interferon-gamma