Does CDP-choline modulate phospholipase activities after transient forebrain ischemia?
Ten min forebrain ischemia/1-day reperfusion resulted in significant decreases in total phosphatidylcholine (PtdCho), phosphatidylinositol (PtdIns), and cardiolipin in gerbil hippocampus. CDP-choline restored cardiolipin levels, arachidonic acid content of PtdCho, partially but significantly restored total PtdCho, and had no effect on PtdIns. These data suggest that CDP-choline prevented the activation of phospholipase A(2) (rather than inhibiting phospholipase A(2) activity) but did not affect activities of PtdCho-phospholipases C and/or D, or phosphoinositide-phospholipase C. CDP-choline also provided significant protection for hippocampal CA(1) neurons.
Department of Neurological Surgery, H4-330, Clinical Science Center, 600 Highland Avenue, University of Wisconsin-Madison, Madison, WI 53792-3232, USA. adibhatl@neurosurg.wisc.edu
A M
AM
Rao
J F
JF
Hatcher
R J
RJ
Dempsey
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D002308:Cardiolipins / Q000737:chemistry / Q000378:metabolism; D003566:Cytidine Diphosphate Choline / Q000008:administration & dosage*; D004195:Disease Models, Animal; D004789:Enzyme Activation / Q000187:drug effects; D005227:Fatty Acids / Q000032:analysis / Q000378:metabolism; D005849:Gerbillinae; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D007274:Injections, Intraperitoneal; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D018697:Nootropic Agents / Q000008:administration & dosage*; D010713:Phosphatidylcholines / Q000737:chemistry / Q000378:metabolism; D010716:Phosphatidylinositols / Q000737:chemistry / Q000378:metabolism; D010739:Phospholipase D / Q000378:metabolism; D010740:Phospholipases / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism; D016548:Prosencephalon / Q000098:blood supply / Q000378:metabolism*; D010738:Type C Phospholipases / Q000378:metabolism
D002308:Cardiolipins; D005227:Fatty Acids; D007527:Isoenzymes; D018697:Nootropic Agents; D010713:Phosphatidylcholines; D010716:Phosphatidylinositols; D016718:Arachidonic Acid; D003566:Cytidine Diphosphate Choline; D010740:Phospholipases; D010741:Phospholipases A; D010738:Type C Phospholipases; D010739:Phospholipase D