Analysis of cellular adhesion by microarray expression profiling.
Microarrays of oligonucleotides or cDNAs can be used to establish the expression profiles of numerous genes in a single experiment. We have established a microarray platform to identify genes in a number of different pathological conditions, particularly those with an inflammation component. This platform utilised the output of an eosinophil sequencing project in which 1069 sequences were identified that were not represented in the public domain. An eosinophil model cell line, AML14.3D10, was used to investigate cell adhesion. The transcription profile of adhered and non-adhered AML 14.3D10 cells was shown to be both technically and biologically reproducible. A number of genes were found differentially expressed in the adhered vs. non-adhered populations. In the adhered population, the expression of these genes was restricted compared to brain, lung, kidney and especially bone marrow. However, the differentially regulated genes were not among those genes most restricted to eosinophils. We discuss the implications of transcription profiling on gene annotation and its potential utility for the identification of targets for drug intervention.
Aventis Pharmaceuticals, Rainham Road South, Dagenham, Essex RM10 7XS, UK.
V
V
Brenner
K
K
Lindauer
A
A
Parkar
J
J
Fordham
I
I
Hayes
M
M
Stow
R
R
Gama
K
K
Pollock
R
R
Jupp
D001483:Base Sequence; D002448:Cell Adhesion / Q000235:genetics*; D002460:Cell Line; D017931:DNA Primers / Q000235:genetics; D004804:Eosinophils / Q000166:cytology / Q000502:physiology; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling / Q000706:statistics & numerical data; D015723:Gene Library; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000706:statistics & numerical data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D014018:Tissue Distribution
D017931:DNA Primers; D012333:RNA, Messenger