The fas locus of the phytopathogen Rhodococcus fascians affects mitosis of tobacco BY-2 cells.
The effect of Rhodococcus fascians, the causal agent of leafy gall disease, on the mitotic behavior of synchronized tobacco Bright Yellow-2 (BY-2) cells was investigated. Incubation of aphidicolin-synchronized BY-2 cells with R. fascians cells specifically resulted in a broader mitotic index peak, an effect that was linked to an intact and expressed fas virulence locus. The obtained results pointed towards an effect of R. fascians on the prophase of mitosis. The relevance of these results to the virulence of the bacterium is discussed.
Temmerman
W
W
Vakgroep Moleculaire Genetica and Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium.
Ritsema
T
T
Simón-Mateo
C
C
Van Montagu
M
M
Mironov
V
V
Inzé
D
D
Goethals
K
K
Holsters
M
M
D002453:Cell Cycle / Q000502:physiology; D002478:Cells, Cultured; D003583:Cytokinins / Q000494:pharmacology; D005798:Genes, Bacterial / Q000502:physiology; D008938:Mitosis / Q000502:physiology; D010947:Plants, Toxic; D011418:Prophase / Q000187:drug effects; D012240:Rhodococcus / Q000235:genetics / Q000502:physiology*; D014026:Nicotiana / Q000166:cytology / Q000382:microbiology*
D003583:Cytokinins