Evidence for the involvement of the GTS1 gene product in the regulation of biological rhythms in the continuous culture of the yeast Saccharomyces cerevisiae.
In the yeast Saccharomyces cerevisiae, ultradian oscillations of energy metabolism have been observed in continuous cultures. Here, we found that the level of the GTS1 gene product oscillated in concert with the ultradian rhythm of energy metabolism. When GTS1 was inactivated by gene disruption, the metabolic oscillation was affected severely, mostly disappearing within a day, in the absence of synchronized stress-response oscillations throughout the continuous culture. The disappearance of biological rhythms in the GTS1-deleted mutant was substantially rescued by transformation with chimera plasmids carrying GTS1 under the control of GTS1's own promoter. On the other hand, this disappearance was not rescued by constitutive expression of GTS1 under the control of the triose phosphate isomerase promoter.
Department of Biochemistry, Yamanashi Medical University, 1110 Shimokato, Tamaho, Nakakoma, 409-3898, Yamanashi, Japan.
J
J
Wang
W
W
Liu
K
K
Mitsui
K
K
Tsurugi
D001683:Biological Clocks / Q000235:genetics* / Q000502:physiology; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D005786:Gene Expression Regulation; D020868:Gene Silencing; D011401:Promoter Regions, Genetic / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors
D005656:Fungal Proteins; C097841:GTS1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors