[Parameters of binding of fluorescent probe pyrrone red with human serum albumin].
The constant of binding of a new fluorescent probe pyrrone red to human serum albumin (Kb = 4.7.10(5) M-1) and the number of binding sites (N = 2) were determined by the method of double fluorimetric titration at a Fex/Fem intensity ratio of 560/625 nm. The affinity of pyrrone red for albumin was by 20% lower than that of 8-anilinonaphthalenesulfonate and 2.4 times higher than that of another probe K35. Thus, pyrrone red is almost identical to amlinonaphthalenesulfonate in affinity for albumin and can be considered as a long-wavelength "red" analogue of anilinonaphthalenesulfonate.
Gavrilov
V B
VB
Institute of Photobiology, Belarussian Academy of Sciences, ul. Akademicheskaya 27, Minsk, 220072 Belarus.
D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D011714:Pyrans / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D011714:Pyrans; D012709:Serum Albumin