Expression of authentic substance P receptors in murine and human dendritic cells.
Recent studies from our laboratory have shown that substance P can elicit transcription factor activation in dendritic cells. In the present study, we extend these findings by demonstrating the presence of authentic substance P (NK-1) receptors on both normal murine and human dendritic cells. Specifically, we demonstrate the presence of mRNA encoding NK-1 tachykinin receptors and have utilized specific antibodies to detect the expression of NK-1 receptor protein in dendritic cells by Western blot analysis and flow cytometry. These data provide a crucial first step in determining the potential of substance P to modulate dendritic cell function.
Marriott
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Department of Biology, 9201 University City Boulevard, University of North Carolina at Charlotte, Charlotte, NC 28223, USA.
Bost
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D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000737:chemistry* / Q000166:cytology / Q000276:immunology*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D015870:Gene Expression / Q000276:immunology; D006801:Humans; D016167:Integrin alphaXbeta2 / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010179:Pancreas / Q000166:cytology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D018040:Receptors, Neurokinin-1 / Q000032:analysis* / Q000235:genetics* / Q000276:immunology; D014158:Transcription, Genetic / Q000276:immunology
D016167:Integrin alphaXbeta2; D012333:RNA, Messenger; D018040:Receptors, Neurokinin-1