Gonadal regulation of PrRP mRNA expression in the nucleus tractus solitarius and ventral and lateral reticular nuclei of the rat.
We investigated the prolactin-releasing peptide (PrRP) gene expression quantitatively in the rat brain and the involvement of estrogen and progesterone using in situ hybridization. The strongest signals were observed in the nucleus tractus solitarius (NTS), which showed approximately 70% of total PrRP mRNA in the brain. Moderate expression was observed in the ventral and lateral reticular nuclei (VLRN) of the medulla oblongata. PrRP mRNA signals in the hypothalamic ventromedial- and dorsomedial nuclei showed only 5% of total signals. The PrRP mRNA expression among female rats showing normal gonadal cycle and male rats showed that the highest levels were in female rats in proestrus. Administration of estrogen or progesterone after ovariectomy induced an increase in PrRP mRNA expression in the NTS. PrRP mRNA content in the NTS increased with the progress of the pregnancy and reached a peak on the 14th day, the mid-period of pregnancy, when plasma progesterone increases. We also observed the colocalization of PrRP and estrogen receptor alpha in the neurons distributed in the NTS by double labeling immunocytochemistry. These findings indicate that PrRP gene expression is regulated by gonadal steroid hormones in the medulla oblongata, and parts of PrRP synthesizing neurons are considered to be directly influenced by estrogen in the NTS.
Kataoka
Y
Y
Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamikyo-ku, 602-8566, Kyoto, Japan.
Iijima
N
N
Yano
T
T
Kakihara
K
K
Hayashi
S
S
Hinuma
S
S
Honjo
H
H
Hayashi
S
S
Tanaka
M
M
Ibata
Y
Y
D000818:Animals; D004031:Diestrus / Q000502:physiology; D047628:Estrogen Receptor alpha; D004967:Estrogens / Q000502:physiology; D005260:Female; D015870:Gene Expression / Q000502:physiology; D007028:Hypothalamic Hormones / Q000235:genetics*; D017403:In Situ Hybridization; D007774:Lactation / Q000502:physiology; D008297:Male; D009474:Neurons / Q000737:chemistry / Q000502:physiology; D009479:Neuropeptides / Q000235:genetics*; D010052:Ovariectomy; D011247:Pregnancy; D011359:Proestrus / Q000502:physiology; D011374:Progesterone / Q000502:physiology; D056690:Prolactin-Releasing Hormone; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D011960:Receptors, Estrogen / Q000032:analysis; D012154:Reticular Formation / Q000166:cytology / Q000502:physiology*; D017552:Solitary Nucleus / Q000166:cytology / Q000502:physiology*
D047628:Estrogen Receptor alpha; D004967:Estrogens; D007028:Hypothalamic Hormones; D009479:Neuropeptides; C534379:Prlh protein, rat; D056690:Prolactin-Releasing Hormone; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D011374:Progesterone