Prostaglandin biosynthesis by fat body from true armyworms, Pseudaletia unipuncta.
We describe prostaglandin (PG) biosynthesis by microsomal-enriched fractions of fat body prepared from true armyworms, Pseudaletia unipuncta. PG biosynthesis was sensitive to experimental conditions, including incubation time, temperature, pH, substrate and protein concentration. Optimal PG biosynthesis conditions included 1 mg of microsomal-enriched protein, incubated at 28 degrees C for 7.5 min at pH 8. These preparations yielded four major PGs: PGA(2), PGE(2), PGD(2) and PGF(2alpha). PGA(2) and PGE(2) were the predominant eicosanoids produced under these conditions. Two non-steroidal anti-inflammatory drugs, indomethacin and naproxen, effectively inhibited PG biosynthesis. Unlike other invertebrate PG biosynthetic systems studied so far, the true armyworm system appeared to be independent of the usual exogenous co-factors required by mammalian and other invertebrate systems. These findings are discussed with respect to PG biosynthesis in other invertebrate and vertebrate systems.
Insect Biochemical Physiology Laboratory, University of Nebraska - Lincoln, Lincoln, NE 68583-0816, USA.
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Tunaz
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Jurenka
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Stanley
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D016718:Arachidonic Acid / Q000378:metabolism; D015237:Dinoprost / Q000096:biosynthesis; D015232:Dinoprostone / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D005216:Fat Body / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D006863:Hydrogen-Ion Concentration; D007213:Indomethacin / Q000494:pharmacology; D008861:Microsomes / Q000378:metabolism; D009036:Moths / Q000378:metabolism*; D009288:Naproxen / Q000494:pharmacology; D015230:Prostaglandin D2 / Q000096:biosynthesis; D011453:Prostaglandins / Q000096:biosynthesis*; D011454:Prostaglandins A / Q000096:biosynthesis; D013696:Temperature
D000894:Anti-Inflammatory Agents, Non-Steroidal; D011453:Prostaglandins; D011454:Prostaglandins A; D016718:Arachidonic Acid; D009288:Naproxen; D015237:Dinoprost; C100008:prostaglandin A2; D015232:Dinoprostone; D015230:Prostaglandin D2; D007213:Indomethacin