Coffee and fitness-coffee suppresses lipopolysaccharide-induced liver injury in rats.
To clarify the relationship between coffee and fitness, we investigated the effect of coffee on weight gain and total cholesterol as well as production of cytokines and activities of GOT (aspartate aminotransferase; EC 2.6.1.1.) and GPT (alanine aminotransferase; EC 2.6.1.2.) as injected lipopolysaccharides. Forty-eight male Wistar rats were divided into three dietary groups (n=16), which were fed a stock diet (control group), the diet supplemented with freeze-dried coffee of 6.2 g/kg (0.62% coffee group), and the diet supplemented with freeze-dried coffee of 13.6 g/kg (1.36% coffee group). It was confirmed by HPLC analysis that the serum caffeine concentrations in both coffee groups became significantly higher in 140 days after the start of feeding. No significant differences in body weight and serum cholesterol were found between the coffee groups and control group, though the coffee groups tended to be somewhat high at cholesterol level. Activities of serum GOT and GPT increased at 2 h after LPS injection, but in the coffee groups were significantly suppressed (p<0.05). However, the coffee feeding could not suppress the increases of serum cytokine (TNF-alpha and IL-6) levels. These results suggest that coffee may serve as a preventive against liver injury.
Department of Biochemistry, Hokkaido University, Sapporo, Japan.
W
W
Sakamoto
J
J
Nishihira
K
K
Fujie
S
S
Mizuno
M
M
Ozaki
S
S
Yukawa
D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D002110:Caffeine / Q000097:blood; D002784:Cholesterol / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D003069:Coffee / Q000009:adverse effects / Q000378:metabolism; D016207:Cytokines / Q000097:blood; D004032:Diet; D020738:Leptin / Q000097:blood; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000633:toxicity*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D008297:Male; D010809:Physical Fitness; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D015430:Weight Gain / Q000187:drug effects
D003069:Coffee; D016207:Cytokines; D020738:Leptin; D008070:Lipopolysaccharides; D002110:Caffeine; D002784:Cholesterol; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase