The evaluation of interdigitated array electrodes for measurement of catecholamines and indoleamines.
The use of Interdigitated Array (IDA) Microelectrodes for detection of low levels of biogenic amines has been demonstrated in stationary solutions and flow systems [M. Morita, et al., Electrochemica Acta 42 (20--21) (1997) 3177--3183]. This technique is highly sensitive. We have evaluated this technology as applied to High Pressure Liquid Chromatography with Electrochemical Detection (HPLC-EC) for analysis of microdialysate and tissue samples. With this new technology we demonstrated a x 10 fold increase in sensitivity in comparison to our existing technology. We are now able to detect dopamine at a level of 53 x 10(-18) moles on column and serotonin at 26 x 10(-18) moles on column. This technology now permits analysis of biogenic amines in samples from brain areas not previously amenable to this type of experiment.
SmithKline Beecham Pharmaceuticals R&D, New Frontiers Science Park, Third Avenue, Harlow, Essex CM19 5AW, UK. john_p_senior@sbphrd.com
J
J
Senior
A
A
Shah
C
C
Monteux
V
V
De Biasi
D001923:Brain Chemistry; D002395:Catecholamines / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D004566:Electrodes; D007211:Indoles / Q000032:analysis*; D010084:Oxidation-Reduction; D012680:Sensitivity and Specificity
D002395:Catecholamines; D007211:Indoles