Regulation of human endometrial stromal decidualization by macrophage colony-stimulating factor.
Decidualized human endometrial stromal cells and human decidual CD56+ cells are reported to produce macrophage colony-stimulating factor (M-CSF), although its physiological roles remain unclear. In this study, the effects of M-CSF on cell viability and 8-Br-cAMP-stimulated decidualization of normal human endometrial stromal cells are examined by using an in vitro decidualization activity assay system. M-CSF alone affected neither the viable cell numbers nor PRL release of nonstimulated stromal cells, while high concentrations of M-CSF strongly suppressed the viable cell numbers and PRL secretion of stromal cells costimulated with 8-Br-cAMP and M-CSF. However, M-CSF did not suppress the viable cell numbers and PRL secretion of 8-Br-cAMP-induced decidualized cells. These results indicate that high concentrations of M-CSF can suppress the cell viability of stromal cells in the decidualization process, and inhibit decidualization of endometrial stromal cells. Thus, nonpregnant decidual M-CSF may autoregulate decidualization and the viable cell numbers of endometrial stromal cells in a paracrine manner.
Department of Obstetrics & Gynecology, Osaka City University Medical School, Osaka, Japan.
T
T
Tanaka
T
T
Sakamoto
N
N
Umesaki
S
S
Ogita
D000704:Analysis of Variance; D017209:Apoptosis; D002478:Cells, Cultured; D003656:Decidua / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004717:Endometrium / Q000166:cytology*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology / Q000502:physiology*; D011247:Pregnancy; D011336:Probability; D011388:Prolactin / Q000187:drug effects / Q000378:metabolism*; D012016:Reference Values; D012680:Sensitivity and Specificity; D017154:Stromal Cells / Q000187:drug effects / Q000502:physiology
D016173:Macrophage Colony-Stimulating Factor; D011388:Prolactin