Amperometric biosensor based on D-aminoacid oxidase for the R-perindopril assay.
A new amperometric biosensor based on D-aminoacid oxidase is described for the assay of R-perindopril. R-perindopril can be determined in the 400-20 nmol/L concentration range; the detection limit is 10 nmol/L. The selectivity was checked with S-perindopril, D- and L-proline, and polyvinylpyrrolidone. The main interfering species was D-proline. An automated system for the assay of R-perindopril based on the concept of flow injection with an amperometric biosensor (based on D-aminoacid oxidase) as detector is also described. The system is suitable for the on-line monitoring of R-perindopril at a sampling rate of 72 samples/h, in the linear range: 100 nmol/L -20 nmol/L with an RSD better than 0.09% (n = 10).
Department of Chemistry, University of Pretoria, South Africa. Koos.vanStaden@chem.up.ac.za
J F
JF
van Staden
R I
RI
Stefan
H Y
HY
Aboul-Enein
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000032:analysis*; D000818:Animals; D015374:Biosensing Techniques; D003605:D-Amino-Acid Oxidase / Q000378:metabolism; D004563:Electrochemistry / Q000379:methods; D004800:Enzymes, Immobilized / Q000378:metabolism; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D020913:Perindopril / Q000032:analysis*; D012680:Sensitivity and Specificity; D013237:Stereoisomerism; D013379:Substrate Specificity; D013552:Swine
D000806:Angiotensin-Converting Enzyme Inhibitors; D004800:Enzymes, Immobilized; D003605:D-Amino-Acid Oxidase; D020913:Perindopril