Recent developments in mucosal delivery of pDNA vaccines.
The development of DNA vaccination to mucosal surfaces has continued apace over the last 2 years, with the investigation of several novel delivery vehicles. There have been advances in the understanding of the basic immunological mechanisms behind the induction of immune responses by plasmid DNA. The mechanistic insights are paving the way for the design of a second generation of mucosally delivered DNA vaccines. This article reviews the recent progress in the field of microparticle, cationic lipid and bacterial delivery systems. All these mechanisms afford some protection from environmental degradation and facilitate DNA uptake. These methods have been compared with respect to transfection efficiency, ability to elicit a full range of immune responses and their relative safety for in vivo applications.
Department of Immunobiology, Guy's, King's and St Thomas' Medical School (GKT), St Thomas Street, London SE1 9RT, UK. linda.klavinskis@kcl.ac.uk
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Barnes
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Barnfield
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Brew
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Klavinskis
D000818:Animals; D001419:Bacteria / Q000235:genetics; D019470:Biolistics; D016503:Drug Delivery Systems; D005822:Genetic Vectors; D006801:Humans; D018928:Immunity, Mucosal; D008055:Lipids; D008863:Microspheres; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology
D008055:Lipids; D019444:Vaccines, DNA