Determination of bromethalin in commercial rodenticides found in consumer product samples by HPLC-UV-vis spectrophotometry and HPLC-negative-ion APCI-MS.
A small amount of green particulate material is encountered in a consumer complaint sample. The green particulates in the sample are identified as a bromethalin-containing rodenticide using high-performance liquid chromatographic (HPLC)-UV-vis spectrophotometric and HPLC-negative-ion atmospheric pressure chemical ionization (APCI)-mass spectrometric (MS) approaches, which are commonly used for the detection and confirmation of bromethalin in grain-based rodenticides. The selective and sensitive nature of the MS detector makes it possible to determine bromethalin without extensive sample cleanup and preconcentration. The estimated detection limit with the UV-vis detector is 500 pg of bromethalin injected into the column. The extensive fragmentation of the bromethalin molecule under APCI conditions provides sufficient structural information for positive identification.
Mesmer
M Z
MZ
United States Food and Drug Administration, Forensic Chemistry Center, Cincinnati, OH 45237-3097, USA.
Flurer
R A
RA
D000814:Aniline Compounds / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005504:Food Analysis; D005506:Food Contamination; D013058:Mass Spectrometry / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000032:analysis; D012378:Rodenticides / Q000032:analysis*
D000814:Aniline Compounds; D004364:Pharmaceutical Preparations; D012378:Rodenticides; C040307:bromethalin