Stimulating effect of an arteriovenous shunt on the in vivo growth of isografted fibroblasts: a preliminary report.
Isogenous fibroblasts derived from the skin of inbred Sprague-Dawley rats were cultured in vitro, labeled with bisbenzamide (BB) or carboxyfluorescein diacetate (CFDA), and seeded into polycarbonate growth chambers. After 24 h incubation in vitro, the chambers, either empty or containing an arteriovenous (AV) shunt, were implanted subcutaneously into the inguinal region of Sprague-Dawley rats and examined by fluorescence microscopy 2 or 7 days later. The AV shunt remained patent in all experiments. The density of labeled cells on the chamber surface in all chambers decreased in the first 2 days after insertion. At 7 days, the cell density in the empty chambers had not altered from the 2-day level, but the density in the AV shunt containing chambers had increased to almost three times the day 2 level (p = 0.013). It appears that an AV shunt can induce a significant proliferation of fibroblasts implanted adjacent to it. For at least 7 days after labeling, BB and CFDA provide a simple and effective method of quantitative detection of implanted fibroblasts. It is concluded that nutrients from the AV shunt implanted in a growth chamber result in a significant increase in the number of viable, matrix-synthesizing cells, compared with AV shunt-free controls.
Bernard O'Brien Institute of Microsurgery, St. Vincent's Hospital Melbourne, Fitzroy, Victoria 3065, Australia.
R A
RA
Mian
K R
KR
Knight
A J
AJ
Penington
J V
JV
Hurley
A
A
Messina
R
R
Romeo
W A
WA
Morrison
D000818:Animals; D001166:Arteriovenous Shunt, Surgical; D001698:Biomedical Engineering / Q000379:methods*; D006690:Bisbenzimidazole / Q000378:metabolism; D002452:Cell Count; D002470:Cell Survival; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D005452:Fluoresceins / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D006801:Humans; D008297:Male; D008856:Microscopy, Fluorescence; D019736:Prostheses and Implants; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000166:cytology
D005452:Fluoresceins; D005456:Fluorescent Dyes; D006690:Bisbenzimidazole