Tumor-derived exosomes are a source of shared tumor rejection antigens for CTL cross-priming.
The initiation of T-cell-mediated antitumor immune responses requires the uptake and processing of tumor antigens by dendritic cells and their presentation on MHC-I molecules. Here we show in a human in vitro model system that exosomes, a population of small membrane vesicles secreted by living tumor cells, contain and transfer tumor antigens to dendritic cells. After mouse tumor exosome uptake, dendritic cells induce potent CD8+ T-cell-dependent antitumor effects on syngeneic and allogeneic established mouse tumors. Therefore, exosomes represent a novel source of tumor-rejection antigens for T-cell cross priming, relevant for immunointerventions.
Immunology Unit, Department of Clinical Biology, Institut Gustave Roussy, Villejuif, France.
J
J
Wolfers
A
A
Lozier
G
G
Raposo
A
A
Regnault
C
C
Théry
C
C
Masurier
C
C
Flament
S
S
Pouzieux
F
F
Faure
T
T
Tursz
E
E
Angevin
S
S
Amigorena
L
L
Zitvogel
D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology; D006801:Humans; D008325:Mammary Neoplasms, Experimental / Q000276:immunology* / Q000648:ultrastructure; D051379:Mice; D016253:Microscopy, Immunoelectron; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm