[Mechanism of transformation in Pichia methanolica yeast: transforming and nontransforming genes].
Two types of genes were found in the study of transformation in yeast Pichia methanolica: transforming (Trg) and nontransforming (Ntg) genes. Transforming genes (P-ADE7,4 and S-LEU2), as linear DNA molecules, can transform competent cells with high efficiency inversely proportional to the molecule size. Nontransforming genes (P-ADE5 and H-LEU2) transform P. methanolica cells at an extremely low rate even when they are combined with transforming genes. The analysis showed that linear DNA molecules with Trg and Ntg can be either rearranged and integrated in random sites of the recipient genome or form circular plasmids, which are capable of autonomous replication irrespective of the presence of specific replicative elements.
State Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, 113545 Russia.
M G
MG
Tarutina
I I
II
Tolstorukov
D017353:Gene Deletion; D005800:Genes, Fungal; D010843:Pichia / Q000235:genetics*; D014170:Transformation, Genetic