[Effects of hypoxic preconditioning on anoxic tolerance and IL-1 beta expression in cultured rat hippocampal neurons].
OBJECTIVE
To study the effects of hypoxic-preconditioning on anoxia-tolerance and IL-1 beta expression in cultured rat hippocampal neurons after anoxia.


METHODS
12 day cultured hippocampal neurons in control and hypoxic-preconditioning group were exposed to anoxic environment(0.9 L/LN2 + 0.1 L/LCO2) for 2, 4, 8 and 12 h. The neurons were immunocytochemically stained using the anti-serum against rhIL-1 beta, The number of survival neurons and the mean optical density (OD) of IL-1 beta expression were investigated.


RESULTS
The morphological changes and IL-1 beta expression induced by acute anoxia in hypoxic-preconditioning neurons were significantly less than those in control. The number of survival neurons was higher in the hypoxia-preconditioning group than that in control group after acute hypoxia.


CONCLUSIONS
Hypoxia-preconditioning can induce the development of anoxia-tolerance in cultured hippocampal neurons. The decreased IL-1 beta in hippocampal neurons may be an adaptive reaction to acute anoxia.
Department of Neurobiology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850.
A S
AS
Ding
F Z
FZ
Wang
M
M
Yu
M
M
Fan
D000818:Animals; D015687:Cell Hypoxia; D002478:Cells, Cultured; D006624:Hippocampus / Q000166:cytology*; D007375:Interleukin-1 / Q000096:biosynthesis*; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar
D007375:Interleukin-1