Interpretation of the detection of Sarcocystis neurona antibodies in the serum of young horses.
Horses that are exposed to Sarcocystis neurona, a causative agent of equine protozoal myeloencephalitis, produce antibodies that are detectable in serum by western blot (WB). A positive test is indicative of exposure to the organism. Positive tests in young horses can be complicated by the presence of maternal antibodies. Passive transfer of maternal antibodies to S. neurona from seropositive mares to their foals was evaluated. Foals were sampled at birth (presuckle), at 24h of age (postsuckle), and at monthly intervals. All foals sampled before suckling were seronegative. Thirty-three foals from 33 seropositive mares became seropositive with colostrum ingestion at 24h of age, confirming that passive transfer of S. neurona maternal antibodies occurs. Thirty-one of the 33 foals became seronegative by 9 months of age, with a mean seronegative conversion time of 4.2 months. These results indicate that evaluation of exposure to S. neurona by WB analysis of serum may be misleading in young horses.
Cook
A G
AG
Department of Large Animal Clinical Sciences, Virginia-Maryland College of Veterinary Medicine, Duck Pond Drive Phase II, Blacksburg, VA 24061, USA. ancook2@vt.edu
Buechner-Maxwell
V
V
Morrow
J K
JK
Ward
D L
DL
Parker
N A
NA
Dascanio
J J
JJ
Ley
W B
WB
Cooper
W
W
D000367:Age Factors; D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis*; D015153:Blotting, Western / Q000662:veterinary; D003126:Colostrum / Q000276:immunology; D005260:Female; D006734:Horse Diseases / Q000175:diagnosis* / Q000469:parasitology; D006736:Horses; D007112:Immunity, Maternally-Acquired; D007074:Immunoglobulin G / Q000032:analysis; D008297:Male; D012522:Sarcocystis / Q000276:immunology / Q000302:isolation & purification*; D012523:Sarcocystosis / Q000175:diagnosis / Q000662:veterinary*; D013997:Time Factors
D000913:Antibodies, Protozoan; D007074:Immunoglobulin G