Characterisation of the putative nucleoprotein gene of infectious salmon anaemia virus (ISAV).
A gene encoding the putative nucleoprotein (NP) of infectious salmon anaemia virus (ISAV), a commercially important salmonid Orthomyxovirus, has been identified. cDNA obtained from a subtractive cDNA library bound specifically to RNA extracted from ISAV-infected SHK-1 cell cultures. The 5' and 3' ends of the gene were amplified using RACE PCR and a full length open reading frame (ORF) of 1851 nt identified encoding a predicted protein of 616 amino acids. No significant homology of this sequence with any other orthomyxovirus nucleoprotein was identifiable using BLAST or FASTA-based database searches. The ISAV-protein was however identified as a nucleoprotein based on its characteristic amino-acid composition. Furthermore the conserved sequence 5' GCAAAGA 3' was identified preceding the ORF, as has been identified in all other ISAV-genes characterised to date.
Snow
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FRS Marine Laboratory, PO Box 101, Victoria Road, AB11 9DB, Aberdeen, UK. snowm@marlab.ac.uk
Cunningham
C O
CO
D000595:Amino Acid Sequence; D000740:Anemia / Q000662:veterinary / Q000821:virology; D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D017124:Conserved Sequence; D005393:Fish Diseases / Q000821:virology; D016679:Genome, Viral; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000235:genetics* / Q000302:isolation & purification; D009975:Orthomyxoviridae / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012327:RNA Virus Infections / Q000662:veterinary; D020317:Salmo salar; D017421:Sequence Analysis; D013045:Species Specificity; D014758:Viral Core Proteins / Q000235:genetics* / Q000302:isolation & purification
D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D014758:Viral Core Proteins