Adherence of eosinophils from allergic and normal ponies to cultured equine endothelial cells.
OBJECTIVE
To compare adherence of stimulated and unstimulated eosinophils from allergic and normal ponies to cultured equine vascular endothelial cells (equine digital vein endothelial cells; EDVEC) and examine the effect of eosinophil-derived factor(s) on cell adherence.


METHODS
Eosinophil adherence to unstimulated EDVEC or EDVEC pretreated with IL-1beta or supernatants from stimulated eosinophils was measured. Supernatants were also assayed for TNFalpha and IL-1beta-like bioactivity.


RESULTS
Adherence of unstimulated and rhIL-5 (10 ng/ml)-stimulated eosinophils from allergic ponies to rhIL-1beta-treated EDVEC was significantly greater than that of cells from normal ponies. Pretreatment of EDVEC with supernatants from stimulated eosinophils from both groups of ponies significantly increased adherence of autologous cells and IL-1beta- and TNFalpha-like bioactivities were detected in the supernatants.


CONCLUSIONS
Mediator-induced activation of equine eosinophils may lead to further eosinophil recruitment by releasing cytokines that up-regulate endothelial cell adhesion molecule expression. Increased adherence of blood eosinophils from allergic ponies to stimulated endothelium could be explained by in vivo priming.
Bailey
S R
SR
Department of Veterinary Basic Sciences, The Royal Veterinary College, Hatfield, Hertfordshire, UK.
Cunningham
F M
FM
D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D004730:Endothelium, Vascular / Q000502:physiology*; D004804:Eosinophils / Q000502:physiology*; D006632:Histamine / Q000494:pharmacology; D006736:Horses; D006967:Hypersensitivity / Q000473:pathology*; D007375:Interleukin-1 / Q000097:blood / Q000494:pharmacology; D015848:Interleukin-5 / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology; D013373:Substance P / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D017077:Culture Media, Conditioned; D007375:Interleukin-1; D015848:Interleukin-5; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D013373:Substance P; D006632:Histamine