Determination of allicin, S-allylcysteine and volatile metabolites of garlic in breath, plasma or simulated gastric fluids.
Various components of garlic and aged garlic extract, including allicin, S-allylcysteine (SAC) and volatile metabolites of allicin were determined in breath, plasma and simulated gastric fluids by HPLC, gas chromatography (GC) or HPLC- and GC-mass spectrometry (MS). Data indicate that allicin decomposes in stomach acid to release allyl sulfides, disulfides and other volatiles that are postulated to be metabolized by glutathione and/or S-adenosylmethionine to form allyl methyl sulfide. SAC can be absorbed by the body and can be determined in plasma by HPLC or HPLC-MS using atmospheric pressure chemical ionization (APCI)-MS.
Rosen
R T
RT
Center for Advanced Food Technology, Cook Campus, Rutgers University, New Brunswick, NJ 08901, USA. rosen@aesop.rutgers.edu
Hiserodt
R D
RD
Fukuda
E K
EK
Ruiz
R J
RJ
Zhou
Z
Z
Lech
J
J
Rosen
S L
SL
Hartman
T G
TG
D000498:Allyl Compounds / Q000378:metabolism; D001944:Breath Tests; D002849:Chromatography, Gas / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003545:Cysteine / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism*; D004220:Disulfides; D005737:Garlic / Q000737:chemistry / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D005766:Gastrointestinal Contents / Q000737:chemistry*; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry / Q000378:metabolism; D010946:Plants, Medicinal; D012436:S-Adenosylmethionine / Q000378:metabolism; D013440:Sulfides / Q000378:metabolism; D013441:Sulfinic Acids / Q000032:analysis / Q000378:metabolism*
D000498:Allyl Compounds; D004220:Disulfides; D010936:Plant Extracts; D013440:Sulfides; D013441:Sulfinic Acids; C006452:allicin; C038491:allyl sulfide; D012436:S-Adenosylmethionine; C065299:S-allylcysteine; D005978:Glutathione; D003545:Cysteine