A novel patatin-like gene stimulated by drought stress encodes a galactolipid acyl hydrolase.
A cDNA (Vupat1) encoding a predicted 43 kDa protein was isolated from drought-stressed cowpea (Vigna unguiculata) leaves. It has homology with patatin, a potato tuber storage protein with lipolytic acyl hydrolase activity. The recombinant protein VUPAT1 expressed in the baculovirus system displays preferentially galactolipid acyl hydrolase activity. Phospholipids are very slowly hydrolyzed and apparently triacylglycerols are not deacylated. Vupat1 promoter contains putative drought-inducible sequences. Northern blots showed that gene expression is stimulated by drought stress and is more pronounced in a drought-sensitive cultivar than in a drought-tolerant one. An involvement in drought-induced galactolipid degradation is proposed for VUPAT1.
Laboratoire de Biochimie et Physiologie de l'Adaptation Végétale, Université Denis Diderot, Paris, France. matos@ccr.jussieu.fr
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AR
Matos
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d'Arcy-Lameta
M
M
França
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S
Pêtres
L
L
Edelman
J
J
Kader
Y
Y
Zuily-Fodil
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AT
Pham-Thi
D016367:Baculoviridae / Q000235:genetics; D015152:Blotting, Northern; D002265:Carboxylic Ester Hydrolases / Q000235:genetics*; D003001:Cloning, Molecular; D004190:Disasters; D038983:Galactolipids; D006017:Glycolipids / Q000378:metabolism*; D018532:Pisum sativum / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D038983:Galactolipids; D006017:Glycolipids; D010940:Plant Proteins; D011994:Recombinant Proteins; C043222:patatin protein, Solanum tuberosum; D002265:Carboxylic Ester Hydrolases