Application of ACC method to synchronous luminiscence: determination of alpha-tocopherol and alpha-tocopheryl acetate in beverages.
A new method based on the Q parameter, that permits the determination of the C(compound A)/C(compound B) ratio without preparing calibration graphs of the two compounds, is proposed. This method has been applied to signals obtained by synchronous luminiscence. Simultaneous determination of alpha-tocopherol and alpha-tocopheryl acetate in beverages using synchronous fluorescence has been carried out. To isolate the compounds from samples, liquid extraction with n-hexane as the organic phase was employed. The presence of interferences was tested using the apparent content curves (ACC) method and the C(alpha-tocopherol)/C(alpha-tocopheryl acetate) ratio was calculated using the Q parameter. The reproducibility and detection limit for the determination of alpha-tocopherol and alpha-tocopheryl acetate were 6.6% and 0.016 mg/L and 1.8% and 0.017 mg/L, respectively.
Pastor-Ferrer
O
O
Departament de Química Analítica, Universitat de València Estudi General, Burjassot, Spain.
Maurí-Aucejo
A R
AR
Llobat-Estellés
M
M
D000085:Acetates; D001628:Beverages / Q000032:analysis*; D006586:Hexanes; D008163:Luminescent Measurements; D008956:Models, Chemical; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012997:Solvents; D013050:Spectrometry, Fluorescence / Q000379:methods / Q000592:standards; D014810:Vitamin E / Q000032:analysis*
D000085:Acetates; D006586:Hexanes; D012997:Solvents; D014810:Vitamin E; C026385:n-hexane