[Alleviation of type I restriction in Escherichia coli K12 in the presence of the arsR gene from pKW301 of Acidiphilium multivorum AIU 301].
A study was made of the antirestriction activity of Acidiphilium multivorum AIU 301 ArsR, a repressor of the ars operon which confers resistance to arsenite and arsenate and is on pKW301. In Escherichia coli, arsR cloned under the control of Plac in a multi-copy vector alleviated restriction of nonmodified lambda DNA by a factor of 120, six times more efficiently than its analogs of conjugal plasmids R64 (incI1) and R773 (incFI). Amino acid sequence analysis showed that the three ArsR proteins have a homologous region of 38 residues, including the antirestriction motif, in their N domains, whereas the motif is in the C domain in the Ard proteins. The other regions are nonhomologous, and pKW301 ArsR is 33 residues shorter than R64 and R773 ArsRs. The total charge is -4 in pKW301 ArsR and +2 in R64 and R733 ArsRs. A total negative charge was assumed to contribute to the antirestriction activity.
S M
SM
Rastorguev
G B
GB
Zavil'gel'skiĭ
K
K
Suzuki
K
K
Sakka
D016947:Acetobacteraceae / Q000235:genetics*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D008969:Molecular Sequence Data; D009876:Operon; D010957:Plasmids; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics*
C489527:ArsR protein, E coli; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D015534:Trans-Activators