Relative potency of cellular and humoral immune responses induced by DNA vaccination.
DNA vaccines can prime broad-based immune responses in small animal models. In the present study, we sought to evaluate the relative ability of DNA vaccines to induce humoral and cellular immune responses. Using a DNA vaccine encoding HIV gag in mice, we observed that CD8+ T cell responses were primed more readily than were antibody responses, particularly at low doses of DNA. These CD8+ T cell responses were detected in spleen cells, as well as at local sites such as the lung and draining lymph nodes. The potency of the HIV gag DNA vaccine used was sufficient to prime strong CTL responses in macaques, but only low to undetectable antibody responses. Therefore, DNA vaccines appear able to prime strong, broad CTL but only modest antibody responses. These results may have implications on the development of vaccines against infectious diseases where both CTL and antibody responses are desired, such as HIV.
Otten
G R
GR
Vaccines Research, Chiron Corporation, Emeryville, California 94608, USA.
Doe
B
B
Schaefer
M
M
Chen
M
M
Selby
M J
MJ
Goldbeck
C
C
Hong
M
M
Xu
F
F
Ulmer
J B
JB
D016915:AIDS Vaccines / Q000235:genetics / Q000276:immunology*; D000818:Animals; D005260:Female; D015683:Gene Products, gag / Q000235:genetics / Q000276:immunology*; D015483:HIV Antibodies / Q000097:blood*; D015658:HIV Infections / Q000517:prevention & control; D015497:HIV-1 / Q000276:immunology; D008253:Macaca mulatta; D051379:Mice; D011498:Protein Precursors / Q000235:genetics / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014611:Vaccination; D019444:Vaccines, DNA / Q000276:immunology*
D016915:AIDS Vaccines; D015683:Gene Products, gag; D015483:HIV Antibodies; D011498:Protein Precursors; D019444:Vaccines, DNA; C059767:p55 gag precursor protein, Human immunodeficiency virus 1