[Effect of berberine on intracellular free CA2+ concentration in cultured brain cells].
Intracellular free calcium concentration ([Ca2+]i) was measured with Ca(2+)-sensitive fluorescent indicator, Fura-2/AM, in cultured brain cells using AR-CM-MIC cation measurement system, and the effects of berberine (Ber) on the changes in [Ca2+]i induced by CaCl2, norepinephrine, KCl and H2O2 were studied. The results indicate that the resting [Ca2+]i was 35 +/- 8 nmol.L-1 in Ca(2+)-free Hank's solution. Ber showed no effect on the resting [Ca2+]i when the extracellular Ca2+ were 0.01-10 mmol.L-1. Ber 1-100 mumol.L-1 dose-dependently inhibited norepinephrine and H2O2 induced [Ca2+]i elevation. Ber at high concentration (10-100 mumol.L-1) inhibited K(+)-induced [Ca2+]i elevation. This suggests that the inhibitory effects of Ber on norepinephrine, K(+)-, and H2O2-induced [Ca2+]i elevation may be one of the mechanisms against cerebral ischemia.
Wu
J F
JF
Department of Pharmacology, Nanjing Medical University, Nanjing 210029.
Liu
T P
TP
Wang
J X
JX
Yang
S J
SJ
D000818:Animals; D001599:Berberine / Q000494:pharmacology*; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D051381:Rats; D017207:Rats, Sprague-Dawley
D001599:Berberine; D002118:Calcium