Induction of virus-specific cytotoxic T lymphocytes by in vivo electric administration of peptides.
Generally, major histocompatibility complex (MHC) class I presentation of peptide antigens only occur for proteins' which are actively synthesized and processed intracellularly, so that immunization with a cytotoxic T lymphocyte (CTL) target peptide does not usually elicit effective CTL responses. In the present study, we explored the use of epitope peptides by in vivo electroporation to introduce directly into the cytoplasm for the vaccine elicitation of virus-specific CTLs in a mouse system. BALB/c mice were immunized with human immunodeficiency virus (HIV) env (P18, residues 311-320) or hepatitis C virus (HCV) NS5 (P17, residues 2423-2434) with or without electric pulses. Effector cells against peptide-labeled target cells were elicited in mice immunized with peptides with electric administration but not without electric administration. Moreover, cytolytic activities of CTL against peptide-labeled target cells were enhanced by the addition of plasmid having the immunostimulatory sequence (ISS) or cDNA of the B7-1 molecule in electric administration of peptides. The results of the present study suggest that a peptide vaccine against a virus using electric administration is effective in eliciting virus specific CTLs.
Department of Bioregulation, Mie University School of Medicine, 2-174 Edobashi, Tsu 514-8507, Mie, Japan.
S
S
Uno-Furuta
S
S
Tamaki
Y
Y
Takebe
S
S
Takamura
A
A
Kamei
G
G
Kim
I
I
Kuromatsu
M
M
Kaito
Y
Y
Adachi
Y
Y
Yasutomi
D000595:Amino Acid Sequence; D000818:Animals; D017951:Antigen Presentation; D000956:Antigens, Viral / Q000008:administration & dosage* / Q000235:genetics; D018122:B7-1 Antigen / Q000235:genetics; D001483:Base Sequence; D018274:Electroporation; D015488:HIV Antigens / Q000008:administration & dosage / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010957:Plasmids / Q000008:administration & dosage / Q000235:genetics; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D017361:Viral Nonstructural Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014764:Viral Proteins / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology; D012324:RNA-Dependent RNA Polymerase
D000956:Antigens, Viral; D018122:B7-1 Antigen; D015488:HIV Antigens; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase