Detection of Treponema denticola in atherosclerotic lesions.
We examined 26 atherosclerotic lesions and 14 nondiseased aorta specimens to detect the periodontopathogenic part of the bacterial 16S rRNA locus by PCR. Treponema denticola sequence of the 16S rRNA locus was found in 6 out of 26 DNA samples (23.1%) from the formalin-fixed, paraffin-embeded atherosclerotic lesions obtained during surgery but not in any of the 14 nondiseased aorta samples from deceased persons. Utilizing immunofluorescence microscopy, we observed aggregated antigenic particles reacting with rabbit antiserum against T. denticola in thin sections of the PCR-positive samples, but we could not detect any reacting particles in the PCR-negative thin sections.
Okuda
K
K
Oral Health Science Center, Tokyo Dental College, 1-2-2, Masago, Mihama-ku, Chiba, Japan. kokuda@tdc.ac.jp
Ishihara
K
K
Nakagawa
T
T
Hirayama
A
A
Inayama
Y
Y
Okuda
K
K
D000328:Adult; D000368:Aged; D001011:Aorta / Q000382:microbiology; D001161:Arteriosclerosis / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis*; D004275:DNA, Ribosomal / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014208:Treponema / Q000235:genetics / Q000302:isolation & purification*; D014211:Treponemal Infections / Q000382:microbiology*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S