Successful xenografting of cryopreserved primary pancreatic cancers.
In order to assess the suitability of cryopreserved neoplastic tissues for xenografting into nude (nu/nu) mice, we compared the take rate in 28 samples of pancreatic ductal carcinoma. Eleven fresh samples were implanted in nu/nu mice, and 17 were frozen in cryopreserving solution and implanted at a later time. All samples were examined for the presence of neoplastic tissue in cryostat sections. A total of 15 tumors grew in the animals; five from the freshly implanted samples and ten from those cryopreserved. Ten xenografted tumors were characterized for alterations in p53, K-ras, and p16 genes, which were found in six, eight, and nine cases, respectively. Our results demonstrate that the take rate for xenografting is comparable between cryopreserved and fresh tissue samples. The procedure allows for the exchange of tumor material between institutions and permits the establishment of centralized facilities for the storage of an array of different primary tumor samples suitable for the production of in vivo models of cancers.
Department of Pathology, Università di Verona, Italy.
C
C
Sorio
A
A
Bonora
S
S
Orlandini
P S
PS
Moore
P
P
Capelli
P
P
Cristofori
G
G
Dal Negro
P
P
Marchiori
G
G
Gaviraghi
M
M
Falconi
P
P
Pederzoli
G
G
Zamboni
A
A
Scarpa
D000328:Adult; D000368:Aged; D000818:Animals; D018273:Carcinoma, Islet Cell / Q000235:genetics / Q000473:pathology*; D015925:Cryopreservation; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D017353:Gene Deletion; D019942:Genes, p16 / Q000235:genetics; D016158:Genes, p53 / Q000235:genetics; D011905:Genes, ras / Q000235:genetics; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D009368:Neoplasm Transplantation; D010183:Pancreatic Ducts / Q000473:pathology*; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000473:pathology*; D016133:Polymerase Chain Reaction; D014183:Transplantation, Heterologous
D004273:DNA, Neoplasm