Identification of a mannitol transporter, AgMaT1, in celery phloem.
A celery petiole phloem cDNA library was constructed and used to identify a cDNA that gives Saccharomyces cerevisiae cells the ability to grow on mannitol and transport radiolabeled mannitol in a manner consistent with a proton symport mechanism. This cDNA was named AgMaT1 (Apium graveolens mannitol transporter 1). The expression profile in source leaves and phloem was in agreement with a role for mannitol in phloem loading in celery. The identification in eukaryotes of a mannitol transporter is important because mannitol is not only a primary photosynthetic product in species such as celery but is also considered a compatible solute and antioxidant implicated in resistance to biotic and abiotic stress.
Noiraud
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N
Laboratoire de Physiologie et Biochimie Végétales, Centre National de la Recherche Scientifique Unité Mixte de Recherches 6161, 40 Avenue du Recteur Pineau, F-86022 Poitiers Cedex, France.
Maurousset
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L
Lemoine
R
R
D000595:Amino Acid Sequence; D019661:Apiaceae / Q000378:metabolism*; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli; D005786:Gene Expression Regulation; D015723:Gene Library; D008353:Mannitol / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D030781:Organisms, Genetically Modified; D010802:Phylogeny; D010940:Plant Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D017385:Sequence Homology; D013379:Substrate Specificity
C432563:AgMat1 protein, Apium graveolens; D002352:Carrier Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D010940:Plant Proteins; D008353:Mannitol