[Nuclear protein e(y)2 from Drosophila melanogaster participates in transcription control].
Molecular analysis of a new evolutionarily conserved transcription factor, e(y)2, was carried out. The protein was detected in a complex of approximately 700 kDa contained in a Drosophila melanogaster transcription nuclear extract. The e(y)2 protein was shown to interact with components of the preinitiation transcription complex TFIID. Addition of e(y)2 to a transcription extract of HeLa cells increased transcription 4-5 times when chromatin, but not free DNA, was used as a template. Genetic analysis showed that the C-terminal amino acid residues of transcription factor TAFII40 are important for its interaction with e(y)2.
Georgieva
S G
SG
Institute of Gene Biology, Russian Academy of Sciences, Moscow, 117334 Russia. tanjal@mx.ibg.relarn.ru
Nabirochkina
E N
EN
Ladygina
N G
NG
Georgiev
P G
PG
Soldatov
A V
AV
D000818:Animals; D004331:Drosophila melanogaster / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism / Q000502:physiology*; D011485:Protein Binding; D035362:Transcription Factor TFIID; D019473:Transcription Factors, TFII / Q000378:metabolism; D014158:Transcription, Genetic
D009687:Nuclear Proteins; D035362:Transcription Factor TFIID; D019473:Transcription Factors, TFII