Imaging the activity of nitrate reductase by means of a scanning electrochemical microscope.
Scanning electrochemical microscopy (SECM) was used to characterize immobilized nitrate reductase (NaR) from Pseudonomonas stutzeri (E.C. 1.7.99.4). Nitrate reductase with membrane fragment was embedded in a polyurethane hydrogel in a capillary and solubilized NaR without membrane fragment was covalently coupled to a diaminoethyl-cellulose-carbamitate film on glass. After systematic studies of possible mediators, SECM feedback imaging of both forms of immobilized NaR was accomplished with methylviologen as redox mediator.
Wilhelm-Ostwald-Institute of Physical and Theoretical Chemistry, University of Leipzig, Germany.
J
J
Zaumseil
G
G
Wittstock
S
S
Bahrs
P
P
Steinrücke
D004800:Enzymes, Immobilized / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D008853:Microscopy / Q000379:methods*; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000737:chemistry / Q000378:metabolism*; D011549:Pseudomonas / Q000201:enzymology
D004800:Enzymes, Immobilized; D008565:Membrane Proteins; D009565:Nitrate Reductases; D050901:Nitrate Reductase