Advances in the selection of transgenic plants using non-antibiotic marker genes.
Production of transgenic plants started more than a decade ago, but it is still a time-consuming operation. One of the critical points is the selection procedure used for the recovery of transgenic shoots after transformation. Moreover, as more transgenic traits are to be incorporated into crops that already have been transformed, it is clear that there is a need for new methods with higher efficiencies. In this article, recently developed selection systems are reviewed. They differ from conventional selection techniques as they are based on supplementing the transgenic cells with a metabolic advantage rather than killing the non-transgenic cells. In many cases, these new selection systems have been found to be superior to conventional methods.
Danisco Biotechnology, Langebrogade 1, DK-1001 Copenhagen K, Denmark.
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Joersbo