Transformation of Curvularia lunata IM 2901 with pAN7-1 influences selected physiological properties of the fungus.
Genetic analysis of Curvularia lunata IM 2901 transformants, previously obtained by electroporation with plasmid pAN7-1, was carried out. Isolates displayed several differences in hygromycin B resistance and their physiology. It was shown that plasmid pAN7-1 was integrated in different copy numbers and at different positions in the genome of the strains studied. Both the wild type and pAN7-1 isolates, when growing in liquid media, produced an extracellular emulsifying agent. The transformants differed in their growth kinetics, intensity of surfactant production and in the efficiency of cortexolone 11beta-hydroxylation, in comparison with the wild type. The micro-organisms varied in susceptibility to the lytic enzyme complex (Novozyme 234), which indicated the presence of differences in their cell wall composition and/or in architecture caused by an integrated plasmid pAN7-1.
Department of Industrial Microbiology, University of Lódź, Poland.
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Kanwal
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Paraszkiewicz
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Długoński
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015139:Blotting, Southern; D003350:Cortodoxone / Q000378:metabolism; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D015245:Deoxyribonuclease BamHI / Q000737:chemistry; D004352:Drug Resistance, Microbial; D004798:Enzymes; D005079:Excipients / Q000378:metabolism; D006921:Hygromycin B / Q000494:pharmacology; D003904:Mitosporic Fungi / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D010957:Plasmids / Q000235:genetics* / Q000502:physiology; D013252:Steroid 11-beta-Hydroxylase / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism; D013501:Surface-Active Agents / Q000378:metabolism; D014170:Transformation, Genetic / Q000502:physiology*
D000900:Anti-Bacterial Agents; D004271:DNA, Fungal; D004798:Enzymes; D005079:Excipients; D013501:Surface-Active Agents; C045154:novozym 234; D006921:Hygromycin B; D013252:Steroid 11-beta-Hydroxylase; D015245:Deoxyribonuclease BamHI; D003350:Cortodoxone