Photoinduced transformation of 14-F-bacteriorhodopsin gelatin films based on both wild type and D96N mutant.
Spectral and kinetic transformations were studied in gelatin films made with 14-F wild type (WT) bacteriorhodopsin (BR) and 14-F D96N mutant BR. Unlike the recent study of water suspensions of the same pigments, where a red shifted species at 660 nm was shown to form under the light in 14-F WT only, there are no drastic differences in photoinduced behavior between gelatin films based on 14-F WT and 14-F D96N. It is not observed any photoinduced formation of red shifted species at 660 nm for both types of films as it is observed for corresponding pigments in water suspension. The observed results are explained in a terms of relationship between the rates of two photoinduced processes that occur in suspensions and films of corresponding pigments. Kinetic characteristics of the photoinduced processes for the films with chemical additives suggest that there are no advantages in using 14-F D96N films when compared to films based on 14-F WT.
Druzhko
A B
AB
Institute of Theoretical and Experimental Biophysics Russian Academy of Sciences, Pushchino, Moscow region 142290, Russia. druzhko@fluor.iteb.serpukhov.su
Shakhbazian
V Y
VY
Alvarez
R
R
de Lera
A R
AR
Weetall
H H
HH
D001436:Bacteriorhodopsins / Q000031:analogs & derivatives* / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects*; D005780:Gelatin; D007700:Kinetics; D010777:Photochemistry; D017354:Point Mutation; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet
C065195:14-fluorobacteriorhodopsin; D001436:Bacteriorhodopsins; D005780:Gelatin