Fission yeast Prp4p kinase regulates pre-mRNA splicing by phosphorylating a non-SR-splicing factor.
We provide evidence that Prp4p kinase activity is required for pre-mRNA splicing in vivo and show that loss of activity impairs G1-S and G2-M progression in the cell cycle. Prp4p interacts genetically with the non-SR (serine/arginine) splicing factors Prp1p and Prp5p. Bacterially produced Prp1p is phosphorylated by Prp4p in vitro. Prp4p and Prp1p also interact in the yeast two-hybrid system. In vivo labelling studies using a strain with a mutant allele of the prp4 gene in the genetic background indicate a change in phosphorylation of the Prp1p protein. These results are consistent with the notion that Prp4p kinase is involved in the control of the formation of active spliceosomes, targeting non-SR splicing factors.
Institute of Genetics, Technical University of Braunschweig, Germany.
W
W
Schwelnus
K
K
Richert
F
F
Opitz
T
T
Gross
Y
Y
Habara
T
T
Tani
N F
NF
Käufer
D000483:Alleles; D053487:DEAD-box RNA Helicases; D005656:Fungal Proteins / Q000378:metabolism; D016193:G1 Phase; D016195:G2 Phase; D016296:Mutagenesis; D009154:Mutation; D010641:Phenotype; D010766:Phosphorylation; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism / Q000502:physiology*; D020365:RNA Helicases / Q000378:metabolism; D012326:RNA Splicing; D000072260:RNA Splicing Factors; D012333:RNA, Messenger / Q000378:metabolism*; D017414:Ribonucleoprotein, U4-U6 Small Nuclear / Q000378:metabolism / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins; D012568:Schizosaccharomyces / Q000201:enzymology* / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins; D017381:Spliceosomes / Q000378:metabolism; D013696:Temperature; D013997:Time Factors; D020798:Two-Hybrid System Techniques
D005656:Fungal Proteins; C109024:PRP1 protein, S pombe; D000072260:RNA Splicing Factors; D012333:RNA, Messenger; D017414:Ribonucleoprotein, U4-U6 Small Nuclear; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D017346:Protein Serine-Threonine Kinases; C489981:prp4 protein, S pombe; C064447:PRP5 protein, S cerevisiae; D053487:DEAD-box RNA Helicases; D020365:RNA Helicases