Serum-free recombinant production of adenovirus using a hollow fiber capillary system.
A novel method for the production of adenoviral vectors on a scale sufficient to support most research applications and early phase clinical trials is presented. This method utilizes serum-free cell culture medium and a hollow fiber cell culture apparatus. Significantly less time and space are required than in conventional methods, and the resulting adenovirus is collected in a much smaller volume, simplifying the purification steps. The protocol described is a reproducible, convenient, biologically safe, and environmentally sound method for the production of adenoviral vectors for laboratory use and has the potential to scale-up the adenovirus production for clinical use.
University of Virginia Health Sciences Center, Charlottesville, VA, USA.
T A
TA
Gardner
S C
SC
Ko
L
L
Yang
J J
JJ
Cadwell
L W
LW
Chung
C
C
Kao
D000256:Adenoviridae / Q000235:genetics / Q000254:growth & development*; D018929:Cell Culture Techniques; D016895:Culture Media, Serum-Free; D005822:Genetic Vectors; D011995:Recombination, Genetic
D016895:Culture Media, Serum-Free