Arginase I expression and activity in human mononuclear cells after injury.
To determine the effect of trauma on arginase, an arginine-metabolizing enzyme, in cells of the immune system in humans.



Arginase, classically considered an enzyme exclusive to the liver, is now known to exist in cells of the immune system. Arginase expression is induced in these cells by cytokines interleukin (IL) 4, IL-10, and transforming growth factor beta, corresponding to a T-helper 2 cytokine profile. In contrast, nitric oxide synthase expression is induced by IL-1, tumor necrosis factor, and gamma interferon, a T-helper 1 cytokine profile. Trauma is associated with a decrease in the production of nitric oxide metabolites and a state of immunosuppression characterized by an increase in the production of IL-4, IL-10, and transforming growth factor beta. This study tests the hypothesis that trauma increases arginase activity and expression in cells of the immune system.



Seventeen severely traumatized patients were prospectively followed up in the intensive care unit for 7 days. Twenty volunteers served as controls. Peripheral mononuclear cells were isolated and assayed for arginase activity and expression, and plasma was collected for evaluation of levels of arginine, citrulline, ornithine, nitrogen oxides, and IL-10.



Markedly increased mononuclear cell arginase activity was observed early after trauma and persisted throughout the intensive care unit stay. Increased arginase activity corresponded with increased arginase I expression. Increased arginase activity coincided with decreased plasma arginine concentration. Plasma arginine and citrulline levels were decreased throughout the study period. Ornithine levels decreased early after injury but recovered by postinjury day 3. Increased arginase activity correlated with the severity of trauma, early alterations in lactate level, and increased levels of circulating IL-10. Increased arginase activity was associated with an increase in length of stay. Plasma nitric oxide metabolites were decreased during this same period.



Markedly altered arginase expression and activity in cells of the human immune system after trauma have not been reported previously. Increased mononuclear cell arginase may partially explain the benefit of arginine supplementation for trauma patients. Arginase, rather than nitric oxide synthase, appears to be the dominant route for arginine metabolism in immune cells after trauma.
Department of Surgery, University of Kentucky College of Medicine, Lexington, Kentucky 40536-0084, USA. jochoa@pop.uky.edu
J B
JB
Ochoa
A C
AC
Bernard
W E
WE
O'Brien
M M
MM
Griffen
M E
ME
Maley
A K
AK
Rockich
B J
BJ
Tsuei
B R
BR
Boulanger
P A
PA
Kearney
S M
SM
Morris
D000328:Adult; D000368:Aged; D001119:Arginase / Q000097:blood*; D015415:Biomarkers; D016022:Case-Control Studies; D002956:Citrulline / Q000097:blood; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000097:blood; D009952:Ornithine / Q000097:blood; D011379:Prognosis; D011446:Prospective Studies; D018709:Statistics, Nonparametric; D015599:Trauma Severity Indices; D014947:Wounds and Injuries / Q000175:diagnosis / Q000276:immunology*
D015415:Biomarkers; D002956:Citrulline; D009569:Nitric Oxide; D009952:Ornithine; D001119:Arginase