Effect of steroid hormones on membrane profiles of HeLa S3 cells.
The cervical carcinoma cell line, HeLa S3, was exposed to oestradiol and progesterone separately and combined in ratios similar to those of the luteal and follicular phases of the menstrual cycle. After 48 h of exposure, membrane proteins were extracted from the plasma membrane and analyzed. The lipid composition of the cells and lectin binding patterns to the cells were also assessed. The hormones had a profound effect on the HeLa S3 membrane protein profiles. Progesterone inhibited the synthesis of a wide variety of proteins while oestradiol negated this effect. Less variation between lipid profiles was noted although progesterone reduced the amount of cholesterol present. Differences in lectin binding profiles between the various treatments were noted, indicating an hormonal effect on glycosylation. Such hormonal effects on membrane composition may have implications in cervical susceptible to microbial infections.
Department of Anatomical Sciences, University of the Witwatersrand Medical School, 7 York Road, Parktown, 2193, South Africa.
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Sommer
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Cowley
D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D002845:Chromatography; D015536:Down-Regulation; D004958:Estradiol / Q000494:pharmacology*; D006031:Glycosylation; D006367:HeLa Cells; D006801:Humans; D037102:Lectins / Q000378:metabolism; D050356:Lipid Metabolism; D011374:Progesterone / Q000494:pharmacology*; D013997:Time Factors; D015854:Up-Regulation
D037102:Lectins; D011374:Progesterone; D004958:Estradiol