Inhibition of gap junctional intercellular communication in rat liver epithelial cells with transforming RNA.
Previous studies indicated that transforming RNA, derived from the 3' half of the U5 small nuclear RNA first stem structure, suppressed the secretory protein translation in vitro. Gap junctions facilitate homeostatic control of cell growth and differentiation and their dysfunction has been correlated with carcinogenesis. Here, we reported that transforming RNA directly suppressed the gap junction protein, connexin 43, translation and thereby inhibited functional gap junction function in rat epithelial cells. Together with previous data, this implies that altered expression of transforming RNA itself is a potential mechanism in inhibiting gap junction function during carcinogenesis.
Department of Pediatrics/Human Development, Institute of Environmental Toxicology and the Center of MSU, Michigan State University, East Lansing 48824, USA.
T
T
Hayashi
J E
JE
Trosko
K
K
Hamada
D000818:Animals; D015153:Blotting, Western; D002450:Cell Communication / Q000187:drug effects / Q000502:physiology*; D002471:Cell Transformation, Neoplastic / Q000378:metabolism; D018031:Connexin 43 / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D017629:Gap Junctions / Q000187:drug effects / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D014176:Protein Biosynthesis / Q000187:drug effects; D012342:RNA, Small Nuclear / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D051381:Rats; D014162:Transfection
D018031:Connexin 43; D012342:RNA, Small Nuclear; C405485:U5 small nuclear RNA