Determination of urinary methylated purine pattern by high-performance liquid chromatography.
We describe the group selective separation and quantification of unmodified and modified purines in human urine by high-performance reverse phase liquid chromatography. The pattern of oxypurines and methylated purines: hypoxanthine (Hx), xanthine (X), 1-methyl hypoxanthine (1-MHx), 1-methyl guanine (1-MG), 3-methyl guanine (3-MG), 7-methyl guanine (7-MG), 1-methyl xanthine (1-MX), 3-methyl xanthine (3-MX), 7-methyl xanthine (7-MX), 1,7-dimethyl guanine (1,7-dMG), 1,3-dimethyl xanthine (1,3-dMX), 1,7-dimethyl xanthine (3,7-dMX) and 1,3,7-trimethyl xanthine (1,3,7-tMX) were determined in a single run in urine of a healthy subject and a gout patient before and after treatment with allopurinol. This method may be useful to investigate the urinary pattern of methylated bases in diseases involving purine metabolism.
Di Pietro
M C
MC
Institute of Biochemistry and Enzymology, University of Siena, Italy.
Vannoni
D
D
Leoncini
R
R
Liso
G
G
Guerranti
R
R
Marinello
E
E
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006073:Gout / Q000378:metabolism; D006801:Humans; D008745:Methylation; D011687:Purines / Q000652:urine*
D011687:Purines