[Correlation study of TGF beta expression in diethylnitrosamine-induced rat liver cancer and mast cells in its vicinity].
OBJECTIVE
To study the correlation between tumor cell expression of TGF beta and the amount of surrounding mast cells in hepatocarcinogenesis.


METHODS
Hepatocarcinoma was induced by diethylnitrosamine (DENA) in rats. The expression of TGF beta was examined immunohistochemically, and mast cells were identified by Alcian blue staining. The results were undergone cell imaging analysis.


RESULTS
Positive expression of TGF beta was observed in the cytoplasm of the hepatocarcinoma cells. The intensity of expression differd in various groups of experiment. There was a positive correlation between the intensity of the TGF beta expression in hepatocarcinoma cells and the amount of mast cells surrounding the tumor nodules (P < 0.01).


CONCLUSIONS
Mast cell infiltration in DENA-induced rat hepatoma may be due to the chemotactic effect of TGF beta expressed in tumor cells.
Department of Pathology, Jiujiang Medical College, Jiujiang 332000, China.
M
M
Zheng
Y
Y
Ruan
Z
Z
Wu
D000818:Animals; D002455:Cell Division; D004052:Diethylnitrosamine / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced* / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D016212:Transforming Growth Factor beta / Q000096:biosynthesis*
D016212:Transforming Growth Factor beta; D004052:Diethylnitrosamine