Complete genomic sequence of the lytic bacteriophage phiYeO3-12 of Yersinia enterocolitica serotype O:3.
phiYeO3-12 is a T3-related lytic bacteriophage of Yersinia enterocolitica serotype O:3. The nucleotide sequence of the 39,600-bp linear double-stranded DNA (dsDNA) genome was determined. The phage genome has direct terminal repeats of 232 bp, a GC content of 50.6%, and 54 putative genes, which are all transcribed from the same DNA strand. Functions were assigned to 30 genes based on the similarity of the predicted products to known proteins. A striking feature of the phiYeO3-12 genome is its extensive similarity to the coliphage T3 and T7 genomes; most of the predicted phiYeO3-12 gene products were >70% identical to those of T3, and the overall organizations of the genomes were similar. In addition to an identical promoter specificity, phiYeO3-12 shares several common features with T3, nonsubjectibility to F exclusion and growth on Shigella sonnei D(2)371-48 (M. Pajunen, S. Kiljunen, and M. Skurnik, J. Bacteriol. 182:5114-5120, 2000). These findings indicate that phiYeO3-12 is a T3-like phage that has adapted to Y. enterocolitica O:3 or vice versa. This is the first dsDNA yersiniophage genome sequence to be reported.
Pajunen
M I
MI
Department of Medical Biochemistry, Institute of Biomedicine, University of Turku, Turku, Finland.
Kiljunen
S J
SJ
Söderholm
M E
ME
Skurnik
M
M
D000595:Amino Acid Sequence; D001433:Bacteriolysis / Q000235:genetics; D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D018965:Frameshifting, Ribosomal; D017353:Gene Deletion; D005814:Genes, Viral; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009154:Mutation; D010442:Peptide Chain Initiation, Translational; D011401:Promoter Regions, Genetic; D018741:Replication Origin; D012260:Ribonucleases / Q000378:metabolism; D017422:Sequence Analysis, DNA; D012703:Serotyping; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D015008:Yersinia enterocolitica / Q000145:classification / Q000821:virology*
D004279:DNA, Viral; D014764:Viral Proteins; D012260:Ribonucleases