Neurotrophic and migratory properties of an olfactory ensheathing cell line.
Olfactory ensheathing cells (OECs) are a unique type of macroglia required for normal olfactory axonal regeneration throughout the lifetime of an individual. Recent evidence in the literature suggests that OECs transplanted into injured spinal cords may facilitate axonal regeneration. In this study, we evaluated the neurotrophic properties of OECs using a homogeneous clonal cell line (nOEC), which does not contain contaminating cell types found in all primary OEC cultures. The results indicate that nOECs express mRNA for NGF, BDNF, NT-4/5, and neuregulins, but not for NT-3 or CNTF. In addition, nOECs secrete NGF, BDNF, and neuregulin, but retain NT-4/5 intracellularly. Finally, prelabeled nOECs derived from rat survived transplantation into a dorsal hemisected region of the hamster spinal cord and migrated only in the injured, dorsal portion of the spinal cord. This migratory pattern suggests that the nOECs are viable in vivo and respond to signals originating from the injured neuronal cells and their processes.
Boruch
A V
AV
Department of Cell Biology, Neurobiology, and Anatomy, Stritch School of Medicine, Loyola University Medical Center, 2160 South First Avenue, Maywood, IL 60153, USA.
Conners
J J
JJ
Pipitone
M
M
Deadwyler
G
G
Storer
P D
PD
Devries
G H
GH
Jones
K J
KJ
D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology*; D006224:Cricetinae; D015870:Gene Expression / Q000502:physiology; D008647:Mesocricetus; D020932:Nerve Growth Factor / Q000235:genetics; D009414:Nerve Growth Factors / Q000235:genetics*; D009416:Nerve Regeneration / Q000502:physiology; D020880:Neuregulins / Q000235:genetics; D009457:Neuroglia / Q000166:cytology* / Q000502:physiology / Q000637:transplantation*; D009831:Olfactory Mucosa / Q000166:cytology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D013119:Spinal Cord Injuries / Q000473:pathology; D014183:Transplantation, Heterologous
D019208:Brain-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D020880:Neuregulins; D012333:RNA, Messenger; C071951:neurotrophin 5; D020932:Nerve Growth Factor; C073922:neurotrophin 4