[Glutamate causes release of nitric oxide from chicken spinal cord in vitro].
Nitric oxide (NO) has been identified to have profound effects on many systems, especially the neural tissues. Many studies suggest that NO mediate the neurotoxicity of glutamate (Glu). In order to determine in vitro whether Glu causes release of NO from chicken spinal cord, different concentrations of Glu (0.1, 0.5, 1.0 mmol.L-1) were added to the primary cultured chicken spinal cord cells, and the quantity of NO- (the metabolism product of NO) in medium was detected. The result shows that Glu can enhance obviously the concentration of NO in primary cell cultures (212% compared with the control). If the spinal cord cells were pretreated with NO synthases (NOS) inhibitor--L-NOARG, the [NO-] was decreased compared with those treated with Glu only, and, on the contrary, the viability of cells was increased. All of the results above indicate that NO may play an important role in the neurotoxicity of Glu on nervous cells. It might be that Glu can instigate the activity of NO synthases, then induce a series of changes within cell and finally lead to the toxic effect on cells.
School of Medicine, Nanjing University, Nanjing 210093.
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Zhou
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Tang
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Zhang
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Zhang
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D018698:Glutamic Acid / Q000494:pharmacology*; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D052248:Nitric Oxide Synthase Type I; D019335:Nitroarginine / Q000494:pharmacology; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism*
D019335:Nitroarginine; D009569:Nitric Oxide; D018698:Glutamic Acid; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I