[Therapeutic effects of combined suicide gene and cytokine gene therapy on erythroleukemia-bearing mice].
OBJECTIVE
To explore the antitumor effect of combined transfer of suicide gene and GM-CSF gene in erythroleukemia-bearing mice.


METHODS
Adenovirus harboring E. coli. cytosine deaminase (CD) gene (Ad-CD) and/or GM-CSF gene (Ad-GM-CSF) were used for the treatment of erythroleukemia-bearing mice. The mice were inoculated with FBL-3 erythroleukemia cells subcutaneously and 3 days later received Ad-CD followed by 5-fluorocytosine (5FC) treatment with or without Ad-GM-CSF.


RESULTS
The mice received Ad-CD/5FC and Ad-GM-CSF developed tumors more slowly and survived much longer than those received Ad-CD/5FC alone, Ad-GM-CSF alone, control virus Ad-LacZ/5FC or PBS. Combined transfer of CD gene and GM-CSF gene induced a higher specific CTL activity than control therapies did. Pathological examination illustrated that there were tumor necrosis and massive lymphocyte infiltration in the mice after the combined therapy.


CONCLUSIONS
Combined transfer of suicide gene and cytokine gene could synergistically inhibit the growth of erythroleukemia cells in the mice and induce tumor specific immunity of the host.
Department of Immunology, Second Military Medical University, Shanghai 200433.
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Ju
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Cao
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Wang
D000260:Adenoviruses, Human / Q000235:genetics; D000818:Animals; D043525:Cytosine Deaminase; D004926:Escherichia coli / Q000235:genetics; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics*; D004915:Leukemia, Erythroblastic, Acute / Q000628:therapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D009700:Nucleoside Deaminases / Q000235:genetics*; D011897:Random Allocation
D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D009700:Nucleoside Deaminases; D043525:Cytosine Deaminase