Adhesion of adenosine diphosphate-activated platelets to human brain microvascular endothelial cells under flow in vitro is mediated via GPIIb/IIIa.
Employing video-enhanced contrast (VEC) microscopy, we examined whether TAK-029 (GPIIb/IIIa antagonist) inhibits the adhesion of activated platelets to human brain microvascular endothelial cells (HBEC) in vitro. HBECs were cultured on a coverglass and put in the observation chamber of VEC microscopy. Then, activated platelets by adenosine diphosphate (ADP) (2 microM) were perfused over HBEC at a low shear rate of 10 s(-1) for 30 min and washed out. Platelets adhered directly to HBEC. However, platelet adhesion to HBEC was suppressed when platelet rich plasma with ADP (2 microM) plus TAK-029 (GPIIb/IIIa antagonist; 1 microM) was perfused over HBEC for 30 min and washed out. Anti-GPIbalpha antibody (GUR20-5) did not inhibit adhesion of ADP-activated platelets to HBEC. The above results showed adhesion of ADP-activated platelets to HBEC under flow in vitro is mediated via GPIIb/IIIa
Department of Neurology, School of Medicine, 35 Shinanomachi, Shinjuku-ku, 160-8582, Tokyo, Japan. tanahasi@med.keio.ac.jp
N
N
Tanahashi
Y
Y
Fukuuchi
M
M
Tomita
Y
Y
Tomita
K
K
Inoue
H
H
Satoh
T
T
Abe
D000244:Adenosine Diphosphate / Q000494:pharmacology; D001921:Brain / Q000098:blood supply*; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000502:physiology; D005343:Fibrinolytic Agents / Q000494:pharmacology*; D006146:Guanidines / Q000494:pharmacology*; D006801:Humans; D008833:Microcirculation; D010973:Platelet Adhesiveness / Q000187:drug effects* / Q000502:physiology; D010974:Platelet Aggregation / Q000187:drug effects / Q000502:physiology; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000037:antagonists & inhibitors* / Q000502:physiology; D011719:Pyrazines / Q000494:pharmacology*
D005343:Fibrinolytic Agents; D006146:Guanidines; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D011719:Pyrazines; D000244:Adenosine Diphosphate; C099142:TAK 029