Gene cloning of immunogenic antigens overexpressed in pancreatic cancer.
The serological analysis of recombinant cDNA expression libraries (SEREX) by utilizing a library derived from a human pancreatic adenocarcinoma cell line and IgG antibodies from an allogeneic patient serum led to the identification of 18 genes: 13 of these were known genes, and 5 were unknown genes. In Northern and RT-PCR analyses, we found that the expression of mRNA of 14 genes was elevated in pancreatic cancer cell lines compared with the levels in normal pancreatic tissues. In addition, the expression of mRNA of hsp105 in colon cancer was greater than that in normal colon tissue. Immunohistochemical analysis using anti-hsp105 antibody revealed that an increased expression of hsp105 is a characteristic feature of pancreatic ductal and colon adenocarcinoma. Furthermore, hsp105 immunoreactivity in some cases of gastric, esophageal, and hepatocellular carcinoma was much stronger than that in normal corresponding tissues. These molecules identified may provide good diagnostic markers for cancer cells.
Nakatsura
T
T
Division of Immunogenetics, Department of Neuroscience and Immunology, Kumamoto University Graduate School of Medical Sciences, 2-2-1 Honjo, Kumamoto, 860-0811, Japan.
Senju
S
S
Yamada
K
K
Jotsuka
T
T
Ogawa
M
M
Nishimura
Y
Y
D000199:Actins / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis / Q000235:genetics*; D015152:Blotting, Northern; D021441:Carcinoma, Pancreatic Ductal / Q000235:genetics / Q000276:immunology / Q000473:pathology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000276:immunology; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D050957:HSP110 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis / Q000235:genetics; D006360:Heat-Shock Proteins / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured
D000199:Actins; D000951:Antigens, Neoplasm; D018076:DNA, Complementary; D050957:HSP110 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; C121993:HSPA4 protein, human; C097205:HSPH1 protein, human; D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D007633:Keratins