Application of HPLC-NMR coupling using C30 phase in the separation and identification of flavonoids of taxonomic relevance.
The on-line separation and identification of two important taxonomic markers for plant species of the Paepalanthus genus, the flavonoids 6-methoxykaempferol-3-O-beta-D-glucopyranoside and 6-methoxykaempferol-3-O-beta-D-6"(p-coumaroyl)glucopyranoside, has been performed with an HPLC-NMR coupling using C30 phase. 1D spectra have been recorded in the stopped-flow mode for the two predominant chromatographic peaks. This is the first application of HPLC-NMR coupling using C30 phase to a taxonomic problem. The technique drastically reduces the required amount of sampling for structure determination.
Instituto de Química de Araraquara, SP, Brazil.
L C
LC
Santos
M
M
Dachtler
F D
FD
Andrade
K
K
Albert
W
W
Vilegas
D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000592:standards; D004867:Equipment Design; D005419:Flavonoids / Q000032:analysis* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy / Q000379:methods / Q000592:standards; D009862:Online Systems; D010946:Plants, Medicinal / Q000737:chemistry / Q000145:classification
D005419:Flavonoids