Expression of epidermal growth factor and platelet-derived growth factor receptors during cervical carcinogenesis.
Altered expression of epidermal growth factor receptor (EGFR) is common in a variety of epithelial malignancies, including cervical cancer. However, the prognostic significance of EGFR expression is controversial for cervical cancer. Platelet-derived growth factor receptor (PDGFR) expression status is unknown in cervical cancer. Our results demonstrated that expression of EGFR and PDGFR was greatly enhanced in vivo and in organotypic cultures of low-grade cervical dysplastic tissues, but levels were decreased in high-grade lesions. To our knowledge, this is the first report identifying the expression of PDGFR in human epithelium. When low-grade dysplastic organotypic culture tissues were induced to differentiate more completely, EGFR expression, but not PDGFR expression, was relocalized to the basal layer as seen in normal tissues. Differentiation also induced phosphorylation of EGFR but not PDGFR. Our results suggest a role for EGFR and PDGFR during the early stages of cervical carcinogenesis, and demonstrate the facility of organotypic cultures to study the role of these growth factors in the development of cervical cancer.
Mayer
T J
TJ
Department of Microbiology and Immunology, Pennsylvania State University College of Medicine. The Milton S. Hershey Medical Center, Hershey 17033, USA.
Frauenhoffer
E E
EE
Meyers
A C
AC
D015153:Blotting, Western; D002471:Cell Transformation, Neoplastic; D066246:ErbB Receptors / Q000378:metabolism* / Q000502:physiology; D005260:Female; D006801:Humans; D010766:Phosphorylation; D011233:Precipitin Tests; D017479:Receptors, Platelet-Derived Growth Factor / Q000378:metabolism* / Q000502:physiology; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000473:pathology
D066246:ErbB Receptors; D017479:Receptors, Platelet-Derived Growth Factor