Simultaneous determination of inulin and p-aminohippuric acid in plasma and urine by reversed-phase high-performance liquid chromatography.
A simple, accurate and sensitive high-performance liquid chromatographic method with UV detection was carried out to measure simultaneously plasma and urine concentrations of both p-aminohippuric acid and inulin. Following a simplified acid hydrolysis of the sample, the separation was carried out in 4 min using a C18 reversed-phase column with a flow-rate of 1 ml/min, and monitoring the absorbance at 280 nm. Within the investigated concentration ranges of inulin (0.1-3.2 mg/ml) and p-aminohippuric acid (0.0097-0.3 mg/ml), good linearity (r>0.99) was obtained. Within-run RSD ranged from 2.9 to 6.1% and between-run RSD ranged from 6.4 to 10%. Analytical recoveries were 101-112%, with little differences between plasma and urine samples. The detection limit was 1 microg/ml for all the analytes studied. This method might be ideal for renal function studies where a rapid and reproducible assessment of both renal glomerular filtration rate and blood flow-rate is required.
Laboratory of Clinical Biochemistry, Children's Hospital and Research Institute Bambino Gesù, Rome, Italy.
A
A
Pastore
S
S
Bernardini
L
L
Dello Strologo
G
G
Rizzoni
C
C
Cortese
G
G
Federici
D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007328:Insulin / Q000032:analysis* / Q000097:blood / Q000652:urine; D007676:Kidney Failure, Chronic / Q000097:blood / Q000652:urine; D008297:Male; D012680:Sensitivity and Specificity; D010130:p-Aminohippuric Acid / Q000032:analysis* / Q000097:blood / Q000652:urine
D007328:Insulin; D010130:p-Aminohippuric Acid