Cloning and Expression of Guanylin from the European eel (Anguilla anguilla).
Extracts of intestinal epithelia from the European eel (Anguilla anguilla) stimulated cGMP production in the T84 human colon carcinoma cell line which suggested the presence of a guanylin-like peptide in this teleost fish. Degenerate oligonucleotide primers were subsequently used in RT-PCR resulting in the amplification, cloning, and sequencing of two cDNAs which represent possible 5' spliceoforms of an eel homologue of the mammalian peptide, guanylin. Northern blotting indicated that the main site of expression of the eel peptide is in the intestine with much lower signals also detected in the kidney. Intestinal expression of guanylin mRNA is up-regulated in both nonmigratory "yellow" and the more sexually mature, migratory "silver" eels following acclimation to the seawater environment. These results suggest that this peptide signalling system may play a role in osmoregulation in euryhaline teleost fish during migration between the marine and freshwater environments.
School of Biology, University of St. Andrews, Bute Medical Buildings, St. Andrews, Fife, United Kingdom.
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Comrie
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Cutler
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D000064:Acclimatization; D000595:Amino Acid Sequence; D000811:Anguilla / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D006152:Cyclic GMP / Q000096:biosynthesis; D005768:Gastrointestinal Hormones; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008969:Molecular Sequence Data; D045265:Natriuretic Peptides; D010455:Peptides / Q000235:genetics* / Q000378:metabolism; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D012623:Seawater; D017386:Sequence Homology, Amino Acid; D014020:Tissue Extracts / Q000494:pharmacology; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D005768:Gastrointestinal Hormones; D045265:Natriuretic Peptides; D010455:Peptides; D011506:Proteins; D012333:RNA, Messenger; D014020:Tissue Extracts; C424130:guanylin, European eel; C072685:guanylin; D006152:Cyclic GMP