Transmission of Borrelia garinii OspA serotype 4 to BALB/c mice by Ixodes ricinus ticks collected in the field.
In Europe, Borrelia garinii OspA serotype 4 has been isolated from the cerebrospinal fluid of patients but, up to now, has never been identified among culture isolates from Ixodes ricinus ticks. This information raises the question of whether OspA serotype 4 is transmitted by I. ricinus in nature. In the present study, I. ricinus nymphs collected in an area of endemicity in southern Germany were allowed to feed on mice. Cultivation of ear biopsy specimens showed that six of seven B. garinii-infected mice were infected by OspA serotype 4. In contrast, very few B. garinii OspA serotype 4 organisms were isolated directly from the ticks which infected the mice; most isolates were B. afzelii. The infected mice transmitted mainly OspA serotype 4 to xenodiagnostic ticks, preferentially in combination with B. afzelii.
Institut de Zoologie, University of Neuchâtel, Emile Argand 11, 2007 Neuchâtel, Switzerland.
C M
CM
Hu
B
B
Wilske
V
V
Fingerle
Y
Y
Lobet
L
L
Gern
D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism*; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D001428:Bacterial Vaccines; D001898:Borrelia / Q000145:classification* / Q000235:genetics / Q000502:physiology*; D001899:Borrelia Infections / Q000382:microbiology* / Q000635:transmission*; D005260:Female; D018884:Ixodes / Q000382:microbiology* / Q000502:physiology; D008074:Lipoproteins; D022123:Lyme Disease Vaccines / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D012703:Serotyping
D000954:Antigens, Surface; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D008074:Lipoproteins; D022123:Lyme Disease Vaccines; C072045:OspA protein