Blocking enzyme-linked immunosorbent assay for detection of antibodies against Actinobacillus pleuropneumoniae serotype 6 in pig serum.
A blocking enzyme-linked immunosorbent assay (ELISA) detecting antibodies against Actinobacillus pleuropneumoniae (Ap) serotype 6 was developed. The blocking ELISA was based on the inhibition of a polyclonal antibody raised against Ap serotype 6. Purified lipopolysaccharide from Ap serotype 6 was used as antigen. The blocking ELISA was tested against sera from pigs experimentally infected with the 12 serotypes of Ap biotype 1. Cross-reaction with serotypes 3 and 8 but not with other serotypes was observed. The sensitivity and specificity of the test on a herd level were evaluated with sera from herds naturally infected with serotypes 2, 6, 8 or 12 and with sera from herds free of infection with any Ap serotype. The blocking ELISA showed a high herd sensitivity (1.00 (0.79-1.00)) and specificity (0.97 (0.93-0.99)).
Klausen
J
J
Danish Veterinary Laboratory, Bülowsvej 27, DK-1790, Copenhagen V, Denmark. jk@svs.dk
Andresen
L O
LO
Barfod
K
K
Sørensen
V
V
D000189:Actinobacillus Infections / Q000175:diagnosis / Q000662:veterinary*; D016977:Actinobacillus pleuropneumoniae / Q000145:classification* / Q000276:immunology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D007106:Immune Sera; D008070:Lipopolysaccharides / Q000276:immunology; D011817:Rabbits; D012680:Sensitivity and Specificity; D012703:Serotyping / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000382:microbiology
D000907:Antibodies, Bacterial; D007106:Immune Sera; D008070:Lipopolysaccharides