High-performance liquid chromatographic determination of the beta2-selective adrenergic agonist fenoterol in human plasma after fluorescence derivatization.
A sensitive high-performance liquid chromatographic method has been developed for the determination of the beta2-selective adrenergic agonist fenoterol in human plasma. To improve the sensitivity of the method, fenoterol was derivatized with N-(chloroformyl)-carbazole prior to HPLC analysis yielding highly fluorescent derivatives. The assay involves protein precipitation with acetonitrile, liquid-liquid-extraction of fenoterol from plasma with isobutanol under alkaline conditions followed by derivatization with N-(chloroformyl)-carbazole. Reversed-phase liquid chromatographic determination of the fenoterol derivative was performed using a column-switching system consisting of a LiChrospher 100 RP 18 and a LiChrospher RP-Select B column with acetonitrile, methanol and water as mobile phase. The limit of quantitation in human plasma was 376 pg fenoterol/ml. The method was successfully applied for the assay of fenoterol in patient plasma.
Kramer
S
S
Institute of Pharmaceutical Chemistry, University of Münster, Germany.
Blaschke
G
G
D058672:Adrenergic beta-2 Receptor Antagonists; D000318:Adrenergic beta-Agonists / Q000097:blood*; D002227:Carbazoles / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005280:Fenoterol / Q000097:blood*; D005453:Fluorescence; D005561:Formates / Q000737:chemistry; D006801:Humans; D012680:Sensitivity and Specificity
D058672:Adrenergic beta-2 Receptor Antagonists; D000318:Adrenergic beta-Agonists; D002227:Carbazoles; D005561:Formates; C427425:N-(chloroformyl)-carbazole; D005280:Fenoterol