[Artificial reconstituted pulmonary surfactant used for prevention and treatment of adult respiratory distress syndrome. II. Bioluminescence method for optimizing formulation].
Based on optimizing the formulations of artificial reconstituted pulmonary surfactant (APS) used for the prevention and treatment of adult respiratory distress syndrome (ARDS) by a surface chemical method, a quartz lung animal model to obtain active alveolar macrophages (AMs) was established, using a bioluminescence technique to optimize APS's formulation according to the free-radical-scavenging abilities of APS. An optimal formulation was obtained, which showed good surface properties and free-radical-scavenging abilities. The APS preparation will be used in ARDS animal model test.
Division of Biopharmaceutics, School of Pharmacy, Shanghai Medical University (SMU), Shanghai 200032.
Z Q
ZQ
Jiang
T X
TX
Hu
Y M
YM
Ma
N Z
NZ
Xi
D000818:Animals; D008163:Luminescent Measurements; D016676:Macrophages, Alveolar / Q000502:physiology*; D008297:Male; D011663:Pulmonary Surfactants / Q000494:pharmacology*; D011791:Quartz; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000494:pharmacology; D012128:Respiratory Distress Syndrome / Q000188:drug therapy; D012829:Silicosis / Q000209:etiology / Q000473:pathology*
D011663:Pulmonary Surfactants; D011994:Recombinant Proteins; D011791:Quartz