[Study of IL-6 signal transduction pathway in a human multiple myeloma cell line--Sko-007].
OBJECTIVE
To investigate the IL-6 signal transduction pathway in a human multiple myeloma (MM) cell line--Sko-007.


METHODS
The electrophoretic mobility shift assay (EMSA) and immunoprecipitation were used to detect the activation of IL-6-related transcription factors(TFs)--STAT3, NF-IL-6, NF-kB and protein kinase ERK, Jak1 in Sko-007 cells stimulated by IL-6.


RESULTS
Both the transcription factors-STAT3, NF-IL-6 and protein kinases-Jak1, ERK were activated by IL-6 in Sko-007 cells.


CONCLUSIONS
The biological function of IL-6 on Sko-007 cells is mediated by the activation of Jak/STAT and Ras/NF-IL-6 signal transduction pathways.
Institute of Basic Medical Science, Academy of Military Medical Science, Beijing 100850, China.
L
L
Song
Y
Y
Li
B
B
Shen
D006801:Humans; D015850:Interleukin-6 / Q000502:physiology*; D009101:Multiple Myeloma / Q000473:pathology / Q000503:physiopathology*; D015398:Signal Transduction; D014407:Tumor Cells, Cultured
D015850:Interleukin-6