[Use of laser flow-type fluorescence aerosol particle counter to evaluate the concentration of microbes in the surface air under high dust content].
The paper deals with the use of a laser flow-type fluorescence aerosol particle counter to evaluate the concentrations of microbes in the surface air under high dust content. Various circuits of flow-type optic aerosol recorders are analyzed. Flow spectral luminescence analysis of some particles flow while exciting the fourth harmonics of a pulse laser on yttrium-aluminium garnet with neodymium by ultraviolet radiation is shown to be the most optimum method for indication of individual aerosol particles. Experiments were conducted on the authors' model of a pilot plant based on this method. The model of a laser flow-type optic analyzer was developed for experimental studies that give a clear display of biological aerosols in complex aerosols. The laser flow-type analyzer-based unit developed may provide a fluorescence signal of aerosol particles in the flow of a sample and that light diffusion signal from them at an exciting light wavelength of 266 nm. Experiments with BVC aerosols and soil dust particles were conducted in different regions of Russia. They showed it possible to detect and to rapidly calculate soil microorganisms by laser flow-type fluorescence assay of individual particles when excited by ultraviolet radiation.
Kalinin
Iu T
IuT
Vorob'ev
S A
SA
Khramov
E N
EN
Vorob'eva
E A
EA
Kuznetsov
A P
AP
Kiselev
O S
OS
D000336:Aerosols / Q000032:analysis*; D000391:Air Microbiology; D000393:Air Pollutants / Q000737:chemistry*; D001419:Bacteria / Q000302:isolation & purification*; D004391:Dust / Q000032:analysis*; D005434:Flow Cytometry / Q000295:instrumentation*; D006801:Humans; D007834:Lasers; D015203:Reproducibility of Results
D000336:Aerosols; D000393:Air Pollutants; D004391:Dust