Centrifuge polarizing microscope. II. Sample biological applications.
The rationale, design and general performance of the CPM (centrifuge polarizing microscope) were described in Part I of this study (Inoué et al. J. Microsc. 201 (2001) 341-356. In this second part, we describe observations on several biological samples that we have explored over the past two years using the CPM. As described in the first part of the study, although the CPM was basically designed as a high-extinction centrifuge polarizing microscope, it also allows observations of the specimen exposed to high centrifugal fields up to 10 500 x g (earth's gravitational acceleration) in fluorescence (532-nm excitation) and in DIC (differential interference or Nomarski contrast).
Inoué
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Marine Biological Laboratory, Woods Hole, MA 02543, U.S.A.
Goda
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Knudson
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D000755:Anemia, Sickle Cell / Q000473:pathology; D000818:Animals; D000845:Annelida / Q000254:growth & development; D002498:Centrifugation; D004023:Dictyostelium / Q000502:physiology; D004912:Erythrocytes / Q000502:physiology; D005306:Fertilization; D051379:Mice; D008859:Microscopy, Polarization / Q000295:instrumentation / Q000379:methods*; D010063:Ovum / Q000502:physiology; D012617:Sea Urchins / Q000254:growth & development