Integration of the Aedes aegypti mosquito genetic linkage and physical maps.
Two approaches were used to correlate the Aedes aegypti genetic linkage map to the physical map. STS markers were developed for previously mapped RFLP-based genetic markers so that large genomic clones from cosmid libraries could be found and placed to the metaphase chromosome physical maps using standard FISH methods. Eight cosmids were identified that contained eight RFLP marker sequences, and these cosmids were located on the metaphase chromosomes. Twenty-one cDNAs were mapped directly to metaphase chromosomes using a FISH amplification procedure. The chromosome numbering schemes of the genetic linkage and physical maps corresponded directly and the orientations of the genetic linkage maps for chromosomes 2 and 3 were inverted relative to the physical maps. While the chromosome 2 linkage map represented essentially 100% of chromosome 2, approximately 65% of the chromosome 1 linkage map mapped to only 36% of the short p-arm and 83% of the chromosome 3 physical map contained the complete genetic linkage map. Since the genetic linkage map is a RFLP cDNA-based map, these data also provide a minimal estimate for the size of the euchromatic regions. The implications of these findings on positional cloning in A. aegypti are discussed.
Brown
S E
SE
Department of Bioagricultural Sciences and Pest Management, College of Agricultural Sciences, Colorado State University, Fort Collins, Colorado 80523, USA.
Severson
D W
DW
Smith
L A
LA
Knudson
D L
DL
D000330:Aedes / Q000235:genetics*; D000818:Animals; D002874:Chromosome Mapping; D003360:Cosmids; D018076:DNA, Complementary / Q000378:metabolism; D015723:Gene Library; D008040:Genetic Linkage; D005819:Genetic Markers; D017403:In Situ Hybridization; D017404:In Situ Hybridization, Fluorescence; D020161:Physical Chromosome Mapping; D012150:Polymorphism, Restriction Fragment Length; D016324:Sequence Tagged Sites
D018076:DNA, Complementary; D005819:Genetic Markers