Terminal differentiation of human promyelocytic leukaemia cells, HL-60, during the G1 period.
Human promyelocytic leukaemic cells, HL-60, arrested in mitosis by nocodazole were released in the presence of 1alpha,25-dihydroxyvitamin D3 and thymidine or hydroxyurea. Cells moved from early G1 period to the G1/S boundary and differentiated. Furthermore, cells arrested at the G1/S boundary by double thymidine block were released, with 1alpha,25-dihydroxyvitamin D3 being added at the end of DNA synthesis. Under the latter conditions, differentiated cells developed, indicating that DNA synthesis is not required for cell differentiation.
Matsuhisa
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Department of Cell Biology, The Osaka Medical Center for Cancer and Cardiovascular Diseases, 1-3-3, Nakamichi, Higashinari, Osaka, 537-8511, Japan.
D002117:Calcitriol / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D004273:DNA, Neoplasm / Q000096:biosynthesis; D016193:G1 Phase; D018922:HL-60 Cells; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology; D015473:Leukemia, Promyelocytic, Acute / Q000378:metabolism / Q000473:pathology*; D015739:Nocodazole / Q000494:pharmacology; D016196:S Phase; D013936:Thymidine / Q000494:pharmacology
D004273:DNA, Neoplasm; D002117:Calcitriol; D015739:Nocodazole; D013936:Thymidine; D006918:Hydroxyurea