Reconstitution of Epstein-Barr virus-based plasmid partitioning in budding yeast.
The EBNA1 protein of Epstein-Barr virus (EBV) mediates the partitioning of EBV episomes and EBV-based plasmids during cell division by a mechanism that appears to involve binding to the cellular EBP2 protein on human chromosomes. We have investigated the ability of EBNA1 and the EBV segregation element (FR) to mediate plasmid partitioning in Saccharomyces cerevisiae. EBNA1 expression alone did not enable the stable segregation of FR-containing plasmids in yeast, but segregation was rescued by human EBP2. The reconstituted segregation system required EBNA1, human EBP2 and the FR element, and functionally replaced a CEN element. An EBP2 binding mutant of EBNA1 and an EBNA1 binding mutant of EBP2 each failed to support FR-plasmid partitioning, indicating that an EBNA1-EBP2 interaction is required. The results provide direct evidence of the role of hEBP2 in EBNA1-mediated segregation and demonstrate that heterologous segregation systems can be reconstituted in yeast.
Department of Medical Genetics and Microbiology, University of Toronto, 1 Kings College Circle, Toronto, Canada M5S 1A8. lori.frappier@utoronto.ca
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D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002877:Chromosomes, Human; D003001:Cloning, Molecular; D019309:Epstein-Barr Virus Nuclear Antigens / Q000235:genetics / Q000502:physiology*; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D009687:Nuclear Proteins; D010957:Plasmids; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins
D002352:Carrier Proteins; C432687:EBNA1BP2 protein, human; C499008:EBP2 protein, S cerevisiae; D019309:Epstein-Barr Virus Nuclear Antigens; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; C417029:EBV-encoded nuclear antigen 1