[Expression of cytotoxic-granule-associated protein TIA-1 in nasal NK/T cell lymphomas and lymphoid hyperplasia].
OBJECTIVE
27 cases of nasal NK/T cell lymphomas were studied for the expression of the cytotoxic-granule-associated protein TIA-1, its immunophenotype, genotype and Epstein-Barr virus infection status.


METHODS
Immunohistochemical staining for TIA-1, CD3, CD56, CD45RO, CD8, CD20; polymerase chain reaction (PCR) for TCR gamma chain gene and immunoglobulin JH chain gene rearrangement analysis; in situ hybridization for EBER 1/2 and double staining for TIA-1, CD8 with EBER 1/2. 10 cases of lymphoid hyperplasia were used for comparison.


RESULTS
(1) In the 27 cases of nasal NK/T cell lymphomas, most tumor cells expressed TIA-1, CD3, CD45RO and EBER 1/2; expression of CD56 was found in 26 cases; no CD8 or CD20 were detected in tumors cells of this series of cases. Double labelled staining showed that the TIA-1 positive tumor cells coexpress EBER 1/2. TCR gamma chain gene rearrangement was detected in only 1 of the 27 cases. (2) In the 10 cases of lymphoid hyperplasia of nasal pharynx, a small amount of TIA-1 positive cells were present in 8 cases and their distribution similar to that of CD8+ cells, in 4 of these cases a few EBER 1/2+ cells were detected, the number of CD45RO+ cells and CD20+ cells were similar in all 10 cases. Double labelled staining showed that the EBER 1/2+ cells did not coexpress TIA-1.


CONCLUSIONS
A high percentage of these tumor cells express TIA-1 and correlate with that of CD56, CD3 epsilon, CD45RO and EBER1/2 in nasal NK/T cell lymphomas. It is suggested that expression of TIA-1 in this tumor may be related to the tumor origin and may also be responsible for its special biologic behavior.
Department of Pathology, West China University of Medical Sciences, Chengdu 610041.
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W
Liu
A C
AC
Chan
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FC
Ho
D000293:Adolescent; D000328:Adult; D000368:Aged; D005260:Female; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D006801:Humans; D016399:Lymphoma, T-Cell / Q000737:chemistry* / Q000235:genetics; D008297:Male; D008565:Membrane Proteins / Q000032:analysis*; D008875:Middle Aged; D009302:Nasopharyngeal Diseases / Q000235:genetics / Q000378:metabolism; D009669:Nose Neoplasms / Q000737:chemistry* / Q000235:genetics; D039102:Poly(A)-Binding Proteins; D011506:Proteins; D019310:Pseudolymphoma / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000032:analysis*; D000074019:T-Cell Intracellular Antigen-1
D008565:Membrane Proteins; D039102:Poly(A)-Binding Proteins; D011506:Proteins; D016601:RNA-Binding Proteins; D000074019:T-Cell Intracellular Antigen-1; C071558:TIA1 protein, human