New high-performance liquid chromatographic method for the detection of picolinic acid in biological fluids.
A HPLC method is described to quantify picolinic acid in milk, blood serum and tissue culture supernatant. The method requires very little sample preparation because acid precipitation allows total recovery of picolinic acid. High specificity and sensitivity were obtained using ion-pair chromatography on a C18 reversed-phase column with tetrabutylammonium hydrogen sulfate as ion pairing reagent. We describe the conditions for the automated testing of multiple samples and for the detection of L-tryptophan and L-kynurenine together with picolinic acid. This system will be utilized to elucidate the relationship between picolinic acid production and human disease. Furthermore, we provide the first evidence of picolinic acid in human blood serum.
Dazzi
C
C
Laboratory of Molecular Biology, G. Gaslini Institute, Genoa Quarto, Italy. molbiol@tin.it
Candiano
G
G
Massazza
S
S
Ponzetto
A
A
Varesio
L
L
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008895:Milk, Human / Q000737:chemistry; D010848:Picolinic Acids / Q000032:analysis* / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D014407:Tumor Cells, Cultured
D010848:Picolinic Acids; C030614:picolinic acid