Unbalanced expression of the different subunits of elongation factor 1 in diabetic skeletal muscle.
In studies using subtraction cloning to screen for alterations in mRNA expression in skeletal muscle from humans with Type 2 diabetes mellitus and control subjects, one of the most prominent differences was in the mRNA for elongation factor (EF)-1alpha. With Northern blot analysis, EF-1alpha expression was enhanced by 2- to 6-fold in both Types 1 and 2 human diabetics. In contrast, no changes in expression of EF-1beta or -gamma were noted. We observed similar results in animal models of Type 1 diabetes. EF-1alpha expression, but not EF-1beta or -gamma expression, was also enhanced in streptozotocin-induced diabetic rats, and this effect was reversed by insulin treatment. An increased level of EF-1alpha mRNA was also observed in nonobese diabetic mice. This unbalanced regulation of the expression of the different subunits of EF-1 may contribute to alterations not only in protein synthesis but also in other cellular events observed in the diabetic state.
Reynet
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Research Division, Joslin Diabetes Center, and Department of Medicine, Harvard Medical School, Boston, MA 02215, USA.
Kahn
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D000818:Animals; D003001:Cloning, Molecular; D003921:Diabetes Mellitus, Experimental / Q000235:genetics* / Q000473:pathology; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics* / Q000473:pathology; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics* / Q000473:pathology; D015870:Gene Expression; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D018482:Muscle, Skeletal / Q000378:metabolism*; D020648:Peptide Elongation Factor 1 / Q000235:genetics*; D012333:RNA, Messenger; D051381:Rats; D017207:Rats, Sprague-Dawley
D020648:Peptide Elongation Factor 1; D012333:RNA, Messenger