[Analysis of the primary structure of hantavirus RNA, detected in organs of patients that died from HFKS in the Republic of Bashkortostan].
Fragments of the minor S and middle M segments of Hantavirus genome RNA were isolated by reverse transcription-polymerase chain reaction (RT-PCR) from the organs of patients who died from hemorrhagic fever with the renal syndrome (HFRS) during an epidemic outbreak of this infection in the Bashkortostan Republic in 1997-1998. The sizes of the resultant PCR fragments were 317 p. n. for S segment and 416 p. n. for M segment. Their primary structures (GENBANK registration numbers AJ133581 and AJ133582) were determined and they were compared with the nucleotide sequences of the respective genome sites of Hantavirus strain CG1820/Ufa-83 isolated from Clethrionomys in Bashkortostan in 1983. The homologies of nucleotide sequences of the corresponding sites of S and M segments of RNA isolate of 1997 and CG1820/Ufa-83 were 87.7 and 96.6%, respectively. The authors discuss the probability of circulation of hantaviruses differing by individual features of primary structure of genome RNA in Bashkortostan.
Nikonorov
Iu M
IuM
Institute of Biochemistry and Genetics, Ufa Research Center, Russian Academy of Sciences, Ufa.
Khasanova
S S
SS
Filatova
O V
OV
Ben'kovskaia
G V
GV
Chemeris
A V
AV
Vakhitov
V A
VA
Tkachenko
E A
EA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002522:Chlorocebus aethiops; D004279:DNA, Viral; D006476:Orthohantavirus / Q000235:genetics*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000737:chemistry* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012426:Russia; D014709:Vero Cells
D004279:DNA, Viral; D012367:RNA, Viral