[Effect of the bidistilled modified water on bovine serum albumin conformation. Fluorescent spectroscopy data].
It was shown that bidistilled modified water induces a marked decrease in the intensity of intrinsic fluorescence of bovine serum albumin and increases the binding of this protein to the fluorescent probe 1.8 ANS. These effects can be interpreted as a denaturing action of bidistilled modified water on the protein and a change in its conformational state, which is probably caused by changes in the microenvironment of the protein molecule. In addition, a substantial increase in the intrinsic fluorescence of bidistilled modified water, as compared with that of distilled water, was found.
Kuvichkin
V V
VV
Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290 Russia.
Novikov
V V
VV
Aliushev
F K
FK
Eremin
S M
SM
Markov
I A
IA
Ten
Iu A
IuA
D004572:Electrolysis; D011487:Protein Conformation; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D055672:Static Electricity; D014867:Water / Q000737:chemistry*
D014867:Water; D012710:Serum Albumin, Bovine