Aging exacerbates hydrogen peroxide-induced alteration of vascular reactivity in rats.
Reactive oxygen species (ROS) such as superoxide anion (O2-*) and hydrogen peroxide (H2O2) can be produced by vascular endothelium and smooth muscle cells under diverse physiological and pathophysiological situations. These species are known to exert various deleterious effects by which they might induce changes in vascular reactivity. The aim of the present study was to evaluate the evolution of vascular susceptibility to H2O2 during aging in rats. Catalase activity was assessed in aortas from young adult (4 months) and aged (24 months) Wistar rats. In parallel experiments, isolated rings from both age groups were exposed to increasing doses of H2O2 (0, 0.1, 1, 5, or 10 mM) for 20 min and the residual vascular response to phenylephrine (PE = 10(-6) M) and acetylcholine (ACh = 10(-6) M) was evaluated. Our results indicate that aging increases aortic catalase activity (4 months: 0.20 +/- 0.02 IU/mg prot versus 24 months: 0.46 +/- 0.06 IU/mg prot, p < 0.001) while it exacerbates vascular sensitivity to H2O2. These results suggest that the observed increased H2O2-induced alterations of vascular reactivity during aging in rats might be due to increased sensitivity of the vasculature to ROS rather than to a decrease in the defense systems against these species.
Laboratoire Stress Cardiovasculaires et Pathologies Associées, Université Joseph Fourier, Grenoble, France.
S
S
Tanguy
F
F
Boucher
M C
MC
Toufektsian
S
S
Besse
J
J
de Leiris
D000109:Acetylcholine / Q000494:pharmacology; D000367:Age Factors; D000375:Aging; D000818:Animals; D001011:Aorta / Q000201:enzymology; D002374:Catalase / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000201:enzymology / Q000502:physiology*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008297:Male; D010656:Phenylephrine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species; D013997:Time Factors
D017382:Reactive Oxygen Species; D010656:Phenylephrine; D006861:Hydrogen Peroxide; D002374:Catalase; D000109:Acetylcholine