Evaluation of a group-specific 16S ribosomal DNA-based PCR for detection of Helicobacter bizzozeronii, Helicobacter felis, and Helicobacter salomonis in fresh and paraffin-embedded gastric biopsy specimens.
A new specific and sensitive 16S ribosomal DNA-based PCR assay was developed. The assay targets a 78-bp DNA fragment unique to Helicobacter bizzozeronii, Helicobacter felis, and Helicobacter salomonis and can be used with freshly frozen and formalin-fixed paraffin-embedded gastric biopsy specimens.
De Groote
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Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium. dominic.degroote@rug.ac.be
Haesebrouck
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van Doorn
L J
LJ
Vandamme
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Ducatelle
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R
D000818:Animals; D001706:Biopsy; D002415:Cats; D002417:Cattle; D004275:DNA, Ribosomal / Q000032:analysis; D004285:Dogs; D016998:Helicobacter / Q000235:genetics / Q000302:isolation & purification*; D016481:Helicobacter Infections / Q000382:microbiology*; D006801:Humans; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D013045:Species Specificity; D013270:Stomach / Q000382:microbiology*
D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S