Allosteric modulation of the GABA(A) receptor in rat hypothalamus by somatostatin is altered by stress.
1. This autoradiographic study was conducted to investigate somatostatin modulation of GABA(A) receptor binding in hypothalamic structures of immobilization-stressed rats. 2. GABA(A) receptor binding was labelled with [35S]-t- butylbicyclophosphorothionate (TBPS), which binds in or near the chloride channel. 3. Several structures of the rat hypothalamus (i.e. the peri- and paraventricular nuclei) display an increase in [35S]-TBPS binding as well as an alteration of the modulatory effect of somatostatin on the GABA(A) receptor complex under stress. Furthermore, these results demonstrate for the first time that somatostatin is particularly effective in modifying [35S]-TBPS binding to the GABA(A) receptor in rat hypothalamus.
Chigr
F
F
Unité Génie Biologique, Faculty of Sciences and Techniques, Beni-Mellal, Morocco.
M'Hamed
S B
SB
Najimi
M
M
D000494:Allosteric Regulation; D000818:Animals; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000378:metabolism; D003292:Convulsants / Q000378:metabolism; D006728:Hormones / Q000378:metabolism / Q000494:pharmacology; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism*; D007103:Immobilization; D010286:Paraventricular Hypothalamic Nucleus / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D011963:Receptors, GABA-A / Q000187:drug effects / Q000378:metabolism*; D013004:Somatostatin / Q000378:metabolism / Q000494:pharmacology; D013312:Stress, Physiological / Q000378:metabolism*
D019086:Bridged Bicyclo Compounds, Heterocyclic; D003292:Convulsants; D006728:Hormones; D011963:Receptors, GABA-A; D013004:Somatostatin; C037476:tert-butylbicyclophosphorothionate