Phase-variable expression of an operon encoding extracellular alkaline protease, a serine protease homolog, and lipase in Pseudomonas brassicacearum.
The rhizobacterium Pseudomonas brassicacearum forms phenotypic variants which do not show extracellular protease and lipase activity. The operon encoding these enzymes, a serine protease homolog, and a type I secretion machinery was characterized. Transcriptional lacZ gene fusions revealed that the expression of the operon is under the control of phase variation.
Chabeaud
P
P
CEA/Cadarache, DSV-DEVM, Laboratoire d'Ecologie Microbienne de la Rhizosphère, UMR 163 CNRS-CEA, F-13108 Saint-Paul-lez-Durance, France.
de Groot
A
A
Bitter
W
W
Tommassen
J
J
Heulin
T
T
Achouak
W
W
D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003360:Cosmids; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D008049:Lipase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D009876:Operon; D011401:Promoter Regions, Genetic; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics* / Q000502:physiology; D011994:Recombinant Proteins / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013381:Subtilisins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D018528:ATP-Binding Cassette Transporters; C088073:AprD protein, Pseudomonas fluorescens; C088074:AprE protein, Bacteria; C088076:AprF protein, Pseudomonas; D001426:Bacterial Proteins; C079999:LipA protein, Bacteria; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D011994:Recombinant Proteins; D008049:Lipase; D013381:Subtilisins