[Analysis of HLA-DQB1 polymorphism by PCR-SSO in Yichu of Yunnan Province].
HLA-DQB1 genes from 76 individuals of Yichu ethnic group in Yunnan Province were investigated, using PCR-SSO genotyping method. Of the 38 DQB1 alleles detected, DQB1 * 0301 (gene frequency: 36.18%-36.84%) was the most common gene. The frequencies of DQB1 * 0502(10.53%-11.18%), DQB1 * 0401 (9.21%), DQB1 * 0302(8.55%-9.21%), DQB1 * 0601(7.89%), DQB1 * 05031(6.58%), and DQB1 * 03032(5.92%-6.58%) are more than 5%. While DQB1 * 0504, DQB1 * 0604, DQB1 * 06052, DQB1 * 0606, DQB1 * 0607, DQB1 * 0608, DQB1 * 06112, DQB1 * 0613, DQB1 * 0615, DQB1 * 0203, DQB1 * 0305, DQB1 * 0306, DQB1 * 0307, and DQB1 * 0308 were not observed. Comparison of HLA-DQB1 allele frequencies of Yichu with those of 13 other Chinese ethnic groups showed some significant differences, suggesting Yichu is unique in the distribution of HLA alleles.
Department of Biochemistry, School of Life Science, Yat-sen University, Guangzhou 510275, China. ls36@zsu.edu.cn
W M
WM
Chen
H L
HL
Huang
Z H
ZH
Liu
H
H
Tao
D J
DJ
Pan
J H
JH
Lin
A L
AL
Xu
D000483:Alleles; D002681:China / Q000208:ethnology; D005838:Genotype; D006683:HLA-DQ Antigens / Q000235:genetics*; D059866:HLA-DQ beta-Chains; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen