Enzymes of Candida albicans cell-wall lytic system produced by Streptomyces thermodiastaticus.
The production of the enzymes of Candida albicans cell-wall lytic system by S. thermodiastaticus was found to be affected by some growth conditions and nutritional factors. The highest lytic activity was obtained after 18 h of incubation at pH 5.5 and an incubation temperature of 50 degrees C. The carbon source influenced the production of the enzymes of the yeast cell wall lytic system. Maximum lytic activity was obtained when Candida albicans cell-wall (1 g/100 ml) was used as the sole carbon source. NaNO3 at 0.1 g/100 ml level was the best nitrogen source for the biosynthesis of the enzymes of the yeast lytic system. From all phosphor sources, microelements, and growth factors tested, KH2PO4 (1 g/l), ZnSO4 (1 mg/l) and Tween 80 (0.1%), respectively were found to favour highest enzymes production of the lytic system. The Candida albicans cell-wall lytic system produced by S. thermodiastaticus mainly contained chitinolytic and proteolytic activities.
Department of Botany, Faculty of Science, Mansoura University, Mansoura, Egypt.
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FA
Mansour
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AH
Mohamedin
D018533:Biomass; D002176:Candida albicans / Q000378:metabolism*; D002364:Caseins / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism*; D002688:Chitinases / Q000378:metabolism; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D006867:Hydrolases / Q000378:metabolism*; D009566:Nitrates / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D011136:Polysorbates / Q000494:pharmacology; D017680:Potassium Compounds / Q000378:metabolism; D013302:Streptomyces / Q000201:enzymology* / Q000254:growth & development; D013379:Substrate Specificity; D013696:Temperature; D019287:Zinc Sulfate / Q000494:pharmacology
D002364:Caseins; D003470:Culture Media; D009566:Nitrates; D010710:Phosphates; D011136:Polysorbates; D017680:Potassium Compounds; D019287:Zinc Sulfate; C031618:sodium nitrate; C013216:potassium phosphate; D006867:Hydrolases; D002688:Chitinases