The effect of high-fat diet on the development of obesity and serum leptin level in rats.
Ten male Wistar-albino rats were overfed with high-fat diet (margarine at the dose 40 g/kg body weight/day in addition to standard rat diet) during six months from the age of between 8-12 weeks. Ten male rats received a standard chow for the same period. The body weights of the overfed rats increased time dependently (basal 171.5 +/- 5, at sixth month 268 +/- 19 g), whereas those of the rats fed with standard chow did not increase significantly (basal 177 +/- 6.4, at sixth month 220 +/- 10 g). At the sixth month, mean body fat percentages were 36.3 +/- 6.7% and 24.2 +/- 5.4% respectively. Both total cholesterol-triglyceride levels and mean serum leptin levels were also higher than in the overfed rats (p < 0.01 and p < 0.05 respectively). The livers of all rats were histopathologically normal.


CONCLUSIONS
High-fat diet resulting in an increased body fat percentage in rats is associated with hyperleptinemia, hypercholesterolemia and hypertriglyceridemia.
University of Dicle, School of Medicine, Departments of Endocrinology and Metabolism, Diyarbakir, Turkey.
M
M
Bahceci
A
A
Tuzcu
M
M
Akkus
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M
Yaldiz
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Ozbay
D000818:Animals; D001823:Body Composition / Q000502:physiology; D001835:Body Weight / Q000502:physiology; D002784:Cholesterol / Q000097:blood; D004041:Dietary Fats / Q000008:administration & dosage*; D002149:Energy Intake / Q000502:physiology; D020738:Leptin / Q000097:blood*; D008297:Male; D009765:Obesity / Q000097:blood / Q000209:etiology*; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000097:blood
D004041:Dietary Fats; D020738:Leptin; D014280:Triglycerides; D002784:Cholesterol