Determination of bromethalin in commercial rodenticides found in consumer product samples by HPLC-UV-vis spectrophotometry and HPLC-negative-ion APCI-MS.
A small amount of green particulate material is encountered in a consumer complaint sample. The green particulates in the sample are identified as a bromethalin-containing rodenticide using high-performance liquid chromatographic (HPLC)-UV-vis spectrophotometric and HPLC-negative-ion atmospheric pressure chemical ionization (APCI)-mass spectrometric (MS) approaches, which are commonly used for the detection and confirmation of bromethalin in grain-based rodenticides. The selective and sensitive nature of the MS detector makes it possible to determine bromethalin without extensive sample cleanup and preconcentration. The estimated detection limit with the UV-vis detector is 500 pg of bromethalin injected into the column. The extensive fragmentation of the bromethalin molecule under APCI conditions provides sufficient structural information for positive identification.
United States Food and Drug Administration, Forensic Chemistry Center, Cincinnati, OH 45237-3097, USA.
M Z
MZ
Mesmer
R A
RA
Flurer
D000814:Aniline Compounds / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005504:Food Analysis; D005506:Food Contamination; D013058:Mass Spectrometry / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000032:analysis; D012378:Rodenticides / Q000032:analysis*
D000814:Aniline Compounds; D004364:Pharmaceutical Preparations; D012378:Rodenticides; C040307:bromethalin