Isolation and characterization of a Bombyx vasa-like gene.
AcDNA for a vasa-like gene (BmVLG) was isolated from Bombyx mori. As with vasa in Drosophila, the expression is restricted to the germline in the larval (and pupal and adult?) stage, and the maternal transcript exists. However, the maternal transcript shows localized (germ anlage) distribution patterns, and after degradation of the transcript during germband formation the transcript within cells clustering in the midst of the germ anlage, which eventually give rise to germ cells, remains and continues to be expressed during subsequent embryogenesis; this contrasts to the case of vasa in Drosophila. These results not only indicate the usefulness of BmVLG as a germ cell marker but also suggest the usefulness of this gene (family) in the study of insect germ anlage formation.
Department of Insect Genetics and Breeding, National Institute of Sericultural and Entomological Science, 1-2 Oowashi, Tsukuba 305-8634, Japan. nakaoh@nises.affrc.go.jp
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics*; D004247:DNA; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D020365:RNA Helicases / Q000737:chemistry / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D004247:DNA; D020365:RNA Helicases