Involvement of histone H4 gene transcription factor 1 in downregulation of vimentin gene expression during skeletal muscle differentiation.
Upon in vitro myogenesis, the intermediate filament protein vimentin is replaced by desmin, the switch in gene expression occurring essentially at the transcriptional level. Trying to elucidate the molecular mechanisms of this genetic control, we show here that the vimentin promoter is specifically recognized and activated by a protein most probably identical to H4TF-1, and that this factor is present in proliferating myoblasts but disappears upon fusion of these cells into multinucleated myotubes. Our results suggest that H4TF-1 is a differentiation stage-specific factor involved in the downregulation of vimentin gene expression during myogenesis.
CNRS UMR 8532, Institut Gustave Roussy, 39 rue Camille Desmoulins, 94800 Villejuif, France. kryszke@igr.fr
M H
MH
Kryszke
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V
Moura-Neto
A
A
Lilienbaum
D
D
Paulin
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C
Auclair
D020816:Amino Acid Motifs; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D015536:Down-Regulation; D017930:Genes, Reporter; D006801:Humans; D051379:Mice; D024510:Muscle Development; D009124:Muscle Proteins / Q000235:genetics* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000166:cytology* / Q000254:growth & development; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000378:metabolism*; D014162:Transfection; D014746:Vimentin / Q000235:genetics* / Q000378:metabolism
C059062:H4TF-1 protein, human; D009124:Muscle Proteins; D014157:Transcription Factors; D014746:Vimentin