The effect of pregnancy on renal clearance of boron in rats given boric acid orally.
Boric acid (H(3)BO(3)) has been shown to cause developmental abnormalities in the offspring of pregnant rats. Comparative data on the renal clearance of boron (B) in rats and humans, both pregnant and nonpregnant, exposed to boric acid (BA) would reduce uncertainty in interspecies extrapolation from rats to humans. The purpose of this study was to evaluate the effect of pregnancy on the plasma half-life and renal clearance of boron in Sprague-Dawley rats given a single oral dose of boric acid. For the half-life study, nonpregnant and pregnant (gestation day 16) rats were given a single dose of 30 mg/kg of boric acid by gavage, and plasma samples were collected at 2-3 h intervals. The plasma half-life of boron was determined to be 2.9 +/- 0.2 and 3.2 +/- 0.3 h in nonpregnant and pregnant rats, respectively. In the clearance study, nonpregnant and pregnant (GD 16) rats were given a single gavage dose of 0.3, 3, or 30 mg/kg of boric acid. Boron clearance was slightly higher in pregnant rats (3.3 +/- 0.6, 3.2 +/- 0.5, and 3.4 +/- 0.5 ml/min/kg, respectively) compared to nonpregnant rats (3.1 +/- 0.8, 3.0 +/- 0.6, and 3.2 +/- 0.5 ml/min/kg, respectively), but the difference was not statistically significant and not dose-related. Boron clearance was less than creatinine clearance, suggesting tubular reabsorption in both groups. In conclusion, pregnancy did not appear to significantly alter the renal clearance or the plasma half-life of boron in Sprague-Dawley rats under the conditions of this study.
Division of Nephrology and Hypertension, Department of Medicine, University of California, Irvine, California 92697, USA. ndvaziri@uci.edu
N D
ND
Vaziri
F
F
Oveisi
B D
BD
Culver
M V
MV
Pahl
M E
ME
Andersen
P L
PL
Strong
F J
FJ
Murray
D000284:Administration, Oral; D000818:Animals; D019540:Area Under Curve; D001888:Boric Acids / Q000008:administration & dosage / Q000493:pharmacokinetics*; D001895:Boron / Q000493:pharmacokinetics* / Q000652:urine; D004305:Dose-Response Relationship, Drug; D005260:Female; D006207:Half-Life; D007668:Kidney / Q000378:metabolism*; D008657:Metabolic Clearance Rate; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000652:urine*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014508:Urea / Q000652:urine
D001888:Boric Acids; D014508:Urea; D001895:Boron; C032688:boric acid