[Aneuploidy and histopathological classification, according to the Musculoskeletal Tumour Society and WHO, of osteosarcoma in children and adolescents].
A flow cytometry analysis in children and adolescents with osteosarcoma was carried out. Investigation concerned 32 fresh surgical specimens (from patients after preoperative chemotherapy) and 18 paraffin-embedded tumour tissues (archive material). The correlation of aneuploidy population parameters to malignancy grading acc. to WHO (GI-GIII) and MSC histopathological classification, was investigated. It was shown that all specimens with aneuploid populations correlated with the histophatological diagnosis of osteosarcoma. Thus cytometry analysis can be a useful and rapid complementary method to conventional histophtatological diagnosis. On the contrary, no correlation was found between the aneuploid parameters and the low grade and high-grade osteosarcoma acc. to WHO and MSC classification. The Multicycle computer system is useful in retrospective analysis of paraffin-embedded tumour tissues. It is suggested that for this kind of investigation fresh diagnostic specimens (before preoperative chemotherapy) would be more adequate.
Samodzielna Pracownia Cytometrii, Instytut Matki i Dziecka w Warszawie, ul. Kasprzaka 17a, 01-211 Warszawa, Polska. cytometr@imid.med.pl
K
K
Rytwinski
T
T
Klepacka
A
A
Wnuk
E
E
Michalak
D000293:Adolescent; D000782:Aneuploidy; D001859:Bone Neoplasms / Q000145:classification / Q000235:genetics* / Q000473:pathology*; D002648:Child; D004273:DNA, Neoplasm; D005434:Flow Cytometry; D006801:Humans; D012516:Osteosarcoma / Q000145:classification / Q000235:genetics* / Q000473:pathology*; D012955:Societies, Medical; D014944:World Health Organization
D004273:DNA, Neoplasm