Microsatellite marker C04107 as a diagnostic marker for copper toxicosis in the Danish population of Bedlington terriers.
The linkage phase of marker C04107 was evaluated before implementation of the marker in a diagnostic test. Blood samples from 68 dogs were collected and genotyped by PCR. Two alleles were detected with sizes of 160 bp and 164 bp and allele frequencies of 0.45 and 0.55 respectively. Genotyping revealed that 35 dogs were heterozygous (51.5%), 22 dogs were homozygous for the normal allele (32.3%) and 11 dogs were homozygous for the disease allele (16.2%). Liver biopsies were taken from 14 selected dogs and the copper content was evaluated histologically. Biopsies from 8 dogs homozygous for the disease allele showed many copper granules along with single cell necrosis, haemosiderosis and cellular infiltration. In liver biopsies from 6 dogs genotyped to be heterozygous or homozygous for the normal allele, copper granules were absent or moderate in number and no lesions were present. The survey demonstrates that the linkage phase of marker C04107 in the Danish population of Bedlington terriers is similar to the linkage phase detected in other countries. Thus, the marker can be used in a diagnostic test for copper toxicosis in Denmark.
Department of Animal Science and Health, The Royal Veterinary and Agricultural University, Frederiksberg, Denmark. hfp@kvl.dk
H F
HF
Proschowsky
B
B
Jepsen
H E
HE
Jensen
A L
AL
Jensen
M
M
Fredholm
D000818:Animals; D003300:Copper / Q000032:analysis / Q000633:toxicity*; D004247:DNA / Q000032:analysis / Q000302:isolation & purification; D003718:Denmark; D004283:Dog Diseases / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D004285:Dogs; D005260:Female; D008040:Genetic Linkage; D005819:Genetic Markers; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008297:Male; D008664:Metal Metabolism, Inborn Errors / Q000175:diagnosis / Q000235:genetics / Q000662:veterinary*; D018895:Microsatellite Repeats; D010375:Pedigree; D016133:Polymerase Chain Reaction / Q000662:veterinary
D005819:Genetic Markers; D003300:Copper; D004247:DNA