Preferential loss of 5q14-21 in intestinal-type gastric cancer with DNA aneuploidy.
BACKGROUND
Little is known about the genetic changes associated with DNA ploidy in gastric cancer (GC). The aim of this study was to identify recurrent or specific chromosomal regions of DNA sequence copy number aberrations (DSCNAs) that might harbor genes associated with DNA aneuploidy in GC.


METHODS
We analyzed DSCNAs with comparative genomic hybridization and DNA ploidy by laser scanning cytometry in 16 primary intestinal-type GCs.


RESULTS
All GCs examined showed at least one DSCNA (loss or gain); eight were DNA diploid (DD) tumors and eight were DNA aneuploid (DA) tumors. The frequent (>30%) DSCNAs were loss of 5q14-21 and gains of 7p11-14, 8q, 20q, and Xq25-26. Recurrent amplifications (>10%) were detected at chromosomal regions 6p, 7p, and 13q. The overall number of DSCNAs was significantly greater in DA than in DD tumors (P = 0.006). Furthermore, the number of aberrations was clearly greater with 5q loss than without 5q loss (P = 0.002). Losses of 5q14-21, 9p21-pter, 16q, and 18q21-qter were preferentially detected in DA tumors.


CONCLUSIONS
The present observations indicate that there is a close relationship between DSCNA and DNA ploidy in intestinal-type GC and that gene(s) at 5q14-21, 9p21-pter, 16q, and/or 18q21-qter may play important roles in acquisition of DNA aneuploidy.
Department of Pathology, Yamaguchi University School of Medicine, Ube, Yamaguchi, Japan.
A
A
Oga
G
G
Kong
Y
Y
Ishii
H
H
Izumi
C Y
CY
Park
K
K
Sasaki
D000328:Adult; D000368:Aged; D000782:Aneuploidy; D002872:Chromosome Deletion; D002895:Chromosomes, Human, Pair 5; D004273:DNA, Neoplasm / Q000032:analysis* / Q000235:genetics; D005260:Female; D006801:Humans; D019044:Image Cytometry; D007834:Lasers; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D013274:Stomach Neoplasms / Q000235:genetics*
D004273:DNA, Neoplasm