FosB, a cysteine-dependent fosfomycin resistance protein under the control of sigma(W), an extracytoplasmic-function sigma factor in Bacillus subtilis.
We demonstrate that the Bacillus subtilis fosB(yndN) gene encodes a fosfomycin resistance protein. Expression of fosB requires sigma(W), and both fosB and sigW mutants are fosfomycin sensitive. FosB is a metallothiol transferase related to the FosA class of Mn(2+)-dependent glutathione transferases but with a preference for Mg(2+) and L-cysteine as cofactors.
Cao
M
M
Department of Microbiology, Cornell University, Ithaca, New York 14853-8101, USA.
Bernat
B A
BA
Wang
Z
Z
Armstrong
R N
RN
Helmann
J D
JD
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D003545:Cysteine / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects; D005578:Fosfomycin / Q000494:pharmacology*; D005982:Glutathione Transferase / Q000502:physiology; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D008969:Molecular Sequence Data; D012808:Sigma Factor / Q000502:physiology*; D014158:Transcription, Genetic
D001426:Bacterial Proteins; C413458:SigW protein, Bacillus subtilis; D012808:Sigma Factor; D005578:Fosfomycin; D008345:Manganese; D005982:Glutathione Transferase; D008274:Magnesium; D003545:Cysteine