Histologic evaluation of skin damage after overlapping and nonoverlapping flashlamp pumped pulsed dye laser pulses: A study on normal human skin as a model for port wine stains.
OBJECTIVE
In the treatment of port wine stains (PWS) with the flashlamp pumped pulsed dye laser (FPPDL), no consensus exists about overlapping of pulses. The advantage of overlapping pulses is homogeneous lightening of the PWS; the risk is redundant tissue damage. The aim of this study was to determine the histopathologic effect on human skin of pulsed dye laser pulses with various degrees of overlap, with normal human skin as a model for PWS.


METHODS
Eighteen healthy white volunteers were irradiated with pulsed dye laser pulses with increasing radiant exposure and with different degrees of overlap. Biopsy samples were taken and histologically analysed.


RESULTS
Overlapping of pulses on normal human skin enhances depth of vascular damage with approximately 30%. Adjacent pulses also show this effect. We found no histologic signs of serious damage to epidermis or dermal connective tissue by using radiant exposure levels of 6-8 J/cm(2), regardless of pulse application.


CONCLUSIONS
Reasoning that the mechanism of tissue injury is comparable for normal and PWS skin, we conclude that it is safe to treat PWS with overlapping FPPDL pulses to achieve homogeneous lightening.
Department of Plastic, Reconstructive, and Hand Surgery, Academic Medical Centre, University of Amsterdam, 1105 AZ, The Netherlands.
P H
PH
Koster
C M
CM
van der Horst
M J
MJ
van Gemert
A C
AC
van der Wal
D000005:Abdomen; D000328:Adult; D001707:Biopsy, Needle; D001940:Breast / Q000601:surgery; D005260:Female; D006801:Humans; D053685:Laser Therapy; D007834:Lasers / Q000009:adverse effects*; D019339:Port-Wine Stain / Q000473:pathology / Q000532:radiotherapy; D011829:Radiation Dosage; D019651:Plastic Surgery Procedures / Q000379:methods; D012016:Reference Values; D012680:Sensitivity and Specificity; D012867:Skin / Q000473:pathology* / Q000528:radiation effects*