AC133 expression on acute myeloid leukemia blasts: correlation to FAB and to CD34 expression and possible implications for peripheral blood progenitor cell purging in AML.
AC133 is an antigen expressed on CD34+ hematopoietic progenitor cells. In acute myeloid leukemia (AML) it is expressed on leukemic blasts of most FAB subtypes. However, few data are available regarding coexpression of other surface antigens. We measured AC133 expression on AML blasts from 28 consecutive patients at initial diagnosis (n=26) or at diagnosis of first relapse (n=2) and on 26 leukapheresis products from 14 patients. In AML AC133 correlated with CD34 expression (Spearman r=0.4711, P=0.0114) and even stronger with combined CD34/CD33 expression (Spearman r=0.5083, P=0.0068). In leukapheresis products AC133 expression correlated with CD34 expression (Spearman r=0.7495, P=0.002) and the yield of the obtained amount of CD34+ cells (Spearman r=0.6484, P=0.0121). In conclusion AC133 expression is closely related to CD34 expression in AML. In leukapheresis products AC133 provides an additional marker for selection of PBPC autografts in AC133- AML.
Department of Hematology, Klinikum der Johann Wolfgang Goethe Universität, Medizinische Klinik III-Hämatologie/Onkologie, Knochenmarktransplantation, Theodor-Stern-Kai 7, 60590 Frankfurt, Germany. f.fauth@em.uni.frankfurt.de
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F
Fauth
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Weidmann
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H
Martin
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B
Schneider
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S
Sonnhoff
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D
Hoelzer
D000071916:AC133 Antigen; D000208:Acute Disease; D000328:Adult; D000368:Aged; D015703:Antigens, CD; D018952:Antigens, CD34 / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000378:metabolism; D016465:Bone Marrow Purging; D005260:Female; D006023:Glycoproteins / Q000096:biosynthesis*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000378:metabolism; D016130:Immunophenotyping; D007937:Leukapheresis; D007951:Leukemia, Myeloid / Q000175:diagnosis* / Q000378:metabolism / Q000628:therapy; D008297:Male; D008875:Middle Aged; D010455:Peptides; D018709:Statistics, Nonparametric; D014182:Transplantation, Autologous
D000071916:AC133 Antigen; D015703:Antigens, CD; D018952:Antigens, CD34; D014408:Biomarkers, Tumor; D006023:Glycoproteins; D007140:Immunoglobulin Fab Fragments; C000606023:PROM1 protein, human; D010455:Peptides