Molecular cloning of Na(+)-ATPase cDNA from a marine alga, Heterosigma akashiwo.
We cloned novel Na(+)-ATPase (HANA) cDNA from marine alga Heterosigma akashiwo. The full-length HANA cDNA was 4467 bp long and coded for a 1330 amino acid protein with a molecular weight of 146,306. The deduced product exhibited around 40% identity in amino acids with Na(+)/K(+)-ATPase alpha-subunits. A hydrophilic sequence of 285 amino acid residues that showed no homology with any sequence listed in databases existed in the M7--M8 junction of HANA. This is the first report on the primary structure of putative Na(+)-transporting ATPase from plant cells.
Japanese International Research Center for Agricultural Sciences-Okinawa Subtropical Station, Ishigaki, Japan. mshono@jircas-os.affrc.go.jp
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Shono
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Wada
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Hara
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Fujii
D000251:Adenosine Triphosphatases / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D027682:Cation Transport Proteins; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis; D056890:Eukaryota / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010802:Phylogeny; D016415:Sequence Alignment; D014871:Water Microbiology
D027682:Cation Transport Proteins; D018076:DNA, Complementary; D000251:Adenosine Triphosphatases; C021838:sodium-translocating ATPase