[The dynamic change of extracellular matrix in human coronary atherogenesis].
OBJECTIVE
To investigate the expression of type I, III procollagen mRNAs and chondroitin sulfate proteoglycan (CSPG) genes, as well as the distribution of type I, III collagen fibers and CSPG in human coronary atherogenesis.


METHODS
Staining for HE, picrosirius red, immunohistochemistry and hybridization in situ technique were included.


RESULTS
(1) Along with the development of atherosclerotic lesion, collagen type I, III and CSPG were increased simultaneously in the coronary wall; The expressions of type I, III procollagen, biglycan, and decorin mRNAs were noticed to be increased in the plaques. (2) beta-lipoprotein was seen coexistently with CSPG in the AS plaques of the coronary arteries. (3) There were more SMC proliferation, predominant augmentation of type III collagen fibers and accumulation of CSPG in the early plaques of most young subjects; but less SMC, amount of type I collagen fibers and deposition of CSPG in the advanced plaques of most aged people.


CONCLUSIONS
Over-expression of type I, III procollagen, biglycan and decorin mRNAs was related to the increase of type I, III collagen and CSPG in the atherosclerotic lesions. Proliferation of SMC, augmentation of type I, III collagen and CSPG and change of the proportion between collagen and CSPG are considered to play an important role in the development of coronary atherosclerosis.
Cardiovascular Institute and Fu Wai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037.
F
F
Yang
P
P
Zhao
M
M
She
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002455:Cell Division; D011508:Chondroitin Sulfate Proteoglycans / Q000378:metabolism*; D003094:Collagen / Q000378:metabolism; D003324:Coronary Artery Disease / Q000209:etiology* / Q000378:metabolism; D003331:Coronary Vessels / Q000378:metabolism; D058575:Decorin; D016326:Extracellular Matrix Proteins; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009131:Muscle, Smooth, Vascular / Q000473:pathology; D011347:Procollagen / Q000096:biosynthesis* / Q000235:genetics; D011509:Proteoglycans / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
D011508:Chondroitin Sulfate Proteoglycans; C546878:DCN protein, human; D058575:Decorin; D016326:Extracellular Matrix Proteins; D011347:Procollagen; D011509:Proteoglycans; D012333:RNA, Messenger; D003094:Collagen