Compartmentalization of Peyer's patch anlagen before lymphocyte entry.
We have shown that Peyer's patch (PP) first develops as a simple and even cell aggregation during embryogenesis. To investigate when and how such a simple cell aggregation forms the complex PP architecture, we analyzed the distribution of cells expressing IL-7R alpha (PP inducer cells), VCAM-1 (mesenchymal cells), CD11c (dendritic cells), and mature lymphocytes by whole-mount immunostaining of 17.5 days post coitus to 2 days postpartum mouse gut. Our results show that compartmentalization of PP anlagen commences at day 18.5 of gestation by clustering and subsequent follicle formation of IL-7R alpha(+), VCAM-1(+), and CD11c(+) cells. This process adds the primitive architecture of PP anlage with several follicles in which IL-7R alpha(+) cells localize in the center, while VCAM-1(+) and CD11c(+) cells localize at the fringe. This follicle formation is accompanied by the establishment of PP-specific vascular network expressing mucosal addressin cellular adhesion molecule-1. Mature B and T lymphocytes entering in the PP anlage are distributed promptly to their own target zones; B cells to the follicle and T cells to nonfollicular zones. Our analysis of scid/scid mouse indicate that the initial processes including formation of PP-specific vascular network occur in the absence of lymphocytes. These observations indicate that the basic architecture of PP is formed by a set of cell lineages assembled during the initial phase of induction of PP anlagen before entry of mature lymphocytes.
Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto, Japan. has@kuhp.kyoto-u.ac.jp
H
H
Hashi
H
H
Yoshida
K
K
Honda
S
S
Fraser
H
H
Kubo
M
M
Awane
A
A
Takabayashi
H
H
Nakano
Y
Y
Yamaoka
S
S
Nishikawa
D000818:Animals; D000831:Animals, Newborn / Q000276:immunology; D015815:Cell Adhesion Molecules; D002449:Cell Aggregation / Q000276:immunology; D002454:Cell Differentiation / Q000276:immunology; D002465:Cell Movement / Q000276:immunology*; D004729:Endothelium, Lymphatic / Q000098:blood supply / Q000196:embryology / Q000276:immunology / Q000378:metabolism; D005260:Female; D007136:Immunoglobulins / Q000096:biosynthesis; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis; D017493:Leukocyte Common Antigens / Q000096:biosynthesis; D016131:Lymphocyte Subsets / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016513:Mice, SCID; D009088:Mucoproteins / Q000096:biosynthesis; D010581:Peyer's Patches / Q000098:blood supply / Q000166:cytology* / Q000196:embryology* / Q000276:immunology; D019707:Receptors, Chemokine / Q000096:biosynthesis; D020395:Receptors, Interleukin-7 / Q000096:biosynthesis; D016201:Receptors, Lymphocyte Homing / Q000096:biosynthesis; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis; D014699:Venules
D015815:Cell Adhesion Molecules; D007136:Immunoglobulins; C489082:Madcam1 protein, mouse; D009088:Mucoproteins; D019707:Receptors, Chemokine; D020395:Receptors, Interleukin-7; D016201:Receptors, Lymphocyte Homing; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D017493:Leukocyte Common Antigens