Apparent intracellular Mg2+ buffering in neurons of the leech Hirudo medicinalis.
The apparent intracellular Mg2+ buffering, or muffling (sum of processes that damp changes in the free intracellular Mg2+ concentration, [Mg2+](i), e.g., buffering, extrusion, and sequestration), was investigated in Retzius neurons of the leech Hirudo medicinalis by iontophoretic injection of H+, OH-, or Mg2+. Simultaneously, changes in intracellular pH and the intracellular Mg2+, Na+, or K+ concentration were recorded with triple-barreled ion-selective microelectrodes. Cell volume changes were monitored measuring the tetramethylammonium (TMA) concentration in TMA-loaded neurons. Control measurements were carried out in electrolyte droplets (diameter 100-200 microm) placed on a silver wire under paraffin oil. Droplets with or without ATP, the presumed major intracellular Mg2+ buffer, were used to quantify the pH dependence of Mg2+ buffering and to determine the transport index of Mg2+ during iontophoretic injection. The observed pH dependence of [Mg2+](i) corresponded to what would be expected from Mg2+ buffering through ATP. The quantity of Mg2+ muffling, however, was considerably larger than what would be expected if ATP were the sole Mg2+ buffer. From the decrease in Mg2+ muffling in the nominal absence of extracellular Na+ it was estimated that almost 50% of the ATP-independent muffling is due to the action of Na+/Mg2+ antiport.
Günzel
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Institut für Neurobiologie, Heinrich-Heine-Universität Düsseldorf, D-40225 Düsseldorf, Germany. guenzel@uni-duesseldorf.de
Zimmermann
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F
Durry
S
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Schlue
W R
WR
D000255:Adenosine Triphosphate / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D017920:Antiporters / Q000378:metabolism; D002021:Buffers; D048429:Cell Size / Q000187:drug effects; D017952:Ganglia, Invertebrate / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007424:Intracellular Fluid / Q000502:physiology; D007700:Kinetics; D007865:Leeches; D008274:Magnesium / Q000494:pharmacology / Q000502:physiology*; D008956:Models, Chemical; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D010042:Ouabain / Q000494:pharmacology; D000644:Quaternary Ammonium Compounds / Q000493:pharmacokinetics / Q000494:pharmacology
D017920:Antiporters; D002021:Buffers; D000644:Quaternary Ammonium Compounds; C046250:sodium-magnesium antiporter; D010042:Ouabain; D000255:Adenosine Triphosphate; C027917:tetramethylammonium; D008274:Magnesium