Dynamic responses of Pseudomonas fluorescens DF57 to nitrogen or carbon source addition.
Cultures of Pseudomonas fluorescens DF57 were grown on different carbon and nitrogen sources. Glucose, succinate and acetate were used as carbon source and pulsed to an aerobic steady-state cultivation of P. fluorescens DF57 at D = 0.1 h(-1) with citrate as limiting carbon source. Glucose was utilised with the fastest uptake rate (19.4 C mmol l(-1) h(-1)) compared to succinate (8.8 C mmol l(-1) h(-1)) and acetate (4.3 C mmol l(-1) h(-1)). Acetate triggered an inhibition of cellular metabolism, which resulted in 2-h long growth arrest after its addition to the steady-state culture. The influence of the nitrogen source was investigated in an aerobic cultivation on a mixture of ammonium and nitrate as limiting nitrogen sources and citrate as non-limiting carbon source. When ammonia and nitrate were pulsed to the steady-state culture, they were mainly assimilated into biomass with a maximum uptake rate of 111 and 33 mg N l(-1) h(-1), respectively. Nitrate uptake was never complete as the residual concentration in the chemostat cultivation was 30 mg N l(-1) nitrate. A pulse of nitrite in the cultivation broth resulted in an inhibition of the growth but not of the primary metabolism, as nitrite was taken up at 38 mg N l(-1) h(-1), citrate was consumed and cofactors were produced continuously. In all experiments, oxygen was used as electron acceptor.
Department of Biotechnology, Center for Process Biotechnology, Building 223, Technical University of Denmark, DK-2800, Lyngby, Denmark.
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D019342:Acetic Acid / Q000008:administration & dosage / Q000378:metabolism; D000332:Aerobiosis; D002244:Carbon / Q000008:administration & dosage / Q000378:metabolism*; D005453:Fluorescence; D005947:Glucose / Q000008:administration & dosage / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D009566:Nitrates / Q000008:administration & dosage / Q000378:metabolism; D009584:Nitrogen / Q000008:administration & dosage / Q000378:metabolism*; D010101:Oxygen Consumption; D011551:Pseudomonas fluorescens / Q000254:growth & development*; D000644:Quaternary Ammonium Compounds / Q000008:administration & dosage / Q000378:metabolism; D019802:Succinic Acid / Q000008:administration & dosage / Q000378:metabolism
D009566:Nitrates; D000644:Quaternary Ammonium Compounds; D009243:NAD; D009249:NADP; D002244:Carbon; D019802:Succinic Acid; D005947:Glucose; D009584:Nitrogen; D019342:Acetic Acid