[Study on telomerase activity in hepatocellular carcinoma and chronic hepatitic disease].
OBJECTIVE
To compare telomerase activity of hepatocellular carcinoma (HCC) with that of chronic liver disease to analyze the significance of telomerase activity in diagnosis of malignancy.


METHODS
Telomerase activity was detected in 38 cases of HCC and corresponding non-tumor liver tissue with different chronic disease. That is 21 cases of hepatic cirrhosis, 2 cases of mild fibrosis, 2 cases of chronic viral hepatitis and 7 cases of non-tumor liver with no significant histopathological changes using TRAP assay. 4 cases of biliary atresia were also detected and 4 cases of normal liver tissue were the control.


RESULTS
Telomerase activity was detected in 32 of 38 (86.8%) cases of HCC. Telomerase activity in HCC was not related with tumor cell differentiation types, tumor size and HBV infection, but expression of telomerase was highly correlated with serum alpha-fetal protein (AFP) level of patients. Telomerase negative HCC group has statistically significant lower level of AFP (P < 0.01) when compared with telomerase positive HCC groups. Telomerase activity was not present in normal liver tissue (0/4), biliary atresia (0/4), mild fibrosis (0/2), chronic viral hepatitis (0/4) and no significant changes of non-tumor liver tissue (0/7). Weak telomerase activity was detected in 4 of 21 hepatic cirrhosis.


CONCLUSIONS
Telomerase activity was only detected in most of HCC and few cases of hepatic cirrhosis, which may play a crucial role in hepatocarcinogenesis and may be useful for the diagnosis of malignancy.
Department of Anatomical and Cellular Patholoty, Chinese University of Hong Kong.
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Wu
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Liew
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Li
D006528:Carcinoma, Hepatocellular / Q000201:enzymology*; D006525:Hepatitis, Viral, Human / Q000201:enzymology; D006801:Humans; D008103:Liver Cirrhosis / Q000201:enzymology; D008113:Liver Neoplasms / Q000201:enzymology*; D019098:Telomerase / Q000378:metabolism*; D000509:alpha-Fetoproteins / Q000378:metabolism
D000509:alpha-Fetoproteins; D019098:Telomerase