Relationship between allozyme genotype and sensitivity to stressors in the western mosquitofish Gambusia affinis detected for elevated temperature but not mercury.
Previous studies with Gambusia holbrooki have found associations of allozyme genotype with tolerance to metals, pesticides, heat, and salinity. To examine the generality of these relationships, we looked for similar associations of mercury and heat tolerance with allozyme genotype at the GPI-2, MDH-1 and MDH-2 loci in its sister species Gambusia affinis. This was done to assess if the loci themselves or closely linked loci were associated with mercury tolerance, because weaker linkage associations would be unlikely to persist across species boundaries. Moreover, the use of two very different types of stress allowed us to determine if the higher tolerance of particular allozyme genotypes is specific to a certain stress or reflects a higher tolerance to stress in general. Associations between genotype and tolerance to mercury and heat were determined in laboratory exposures of about 875 fish in each of two exposures where tolerance was measured as time-to-death, followed by electrophoresis on cellulose acetate gels. For none of the three loci did we find an association of genotype with tolerance to mercury. This contrasts with reports of such an association for GPI-2 and MDH-1 in G. holbrooki, so our results do not support the hypothesis that observed associations between allozyme genotypes and mercury tolerance are due to the allozymes or closely linked loci. However, our comparison was weakened by a scarcity of the GPI-2 genotypes reported to be mercury sensitive in G. holbrooki. Furthermore, rapid mortality in our mercury exposure may have affected the ability to detect genotypic differences in survival. The MDH-1 heterozygote showed higher tolerance to heat stress compared to homozygotes, although this difference was only significant for the most common homozygous genotype. No such relationship between MDH-1 and heat stress has been reported in G. holbrooki. We found no evidence that associations between allozyme genotype and tolerance are similar for different types of stresses, which could be an advantage for using allozymes as an indicator of exposure history to a stressor. Our study also showed that G. affinis sex and weight influence tolerance to mercury and heat.
Department of Biology, University of Louisiana at Lafayette, P.O. Box 42451, Lafayette, LA 70504, USA. lewisss@email.uc.edu
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D019747:Aldose-Ketose Isomerases / Q000235:genetics; D000483:Alleles; D000818:Animals; D003532:Cyprinodontiformes / Q000235:genetics*; D005260:Female; D005393:Fish Diseases / Q000201:enzymology* / Q000235:genetics*; D005649:Fumarate Hydratase / Q000235:genetics; D020022:Genetic Predisposition to Disease; D005838:Genotype; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics; D018882:Heat Stress Disorders / Q000201:enzymology / Q000235:genetics / Q000662:veterinary*; D007521:Isocitrate Dehydrogenase / Q000235:genetics; D007770:L-Lactate Dehydrogenase / Q000235:genetics; D008291:Malate Dehydrogenase / Q000235:genetics; D008297:Male; D008630:Mercury Poisoning / Q000201:enzymology / Q000235:genetics / Q000662:veterinary*; D010734:Phosphogluconate Dehydrogenase / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique / Q000662:veterinary
D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D010734:Phosphogluconate Dehydrogenase; D005649:Fumarate Hydratase; D019747:Aldose-Ketose Isomerases; C032728:mannose isomerase; D005956:Glucose-6-Phosphate Isomerase