Poly(L-lysine)-GRGDS as a biomimetic surface modifier for poly(lactic acid).
The immobilization of adhesion peptide sequences (such as RGD) at the surfaces of poly(alpha-hydroxyacid)s, including poly(lactic acid) (PLA), is complicated by an absence of functional groups to support covalent attachment. We demonstrate a method to overcome this problem, by attaching the peptide to poly(L-lysine) (PLL), which immobilizes the sequence through adsorption at the poly(alpha-hydroxyacid) surface. When coated using a 0.01% w/v solution of PLL-GRGDS, bovine aortic endothelial cells seeded upon the modified PLA showed a marked increase in spreading over unmodified PLA. However, inhibition of the cell-spreading effect occurred when using higher concentrations of PLL-GRGDS, which we attribute to the PLL component. This inhibitory effect can be challenged by increasing the amount of GRGDS attached to each PLL molecule. Potentially, this is a flexible method of surface modification that can engineer many different types of tissue engineering scaffolds with a variety of biomolecules, thus allowing initial cell adhesion to be controlled.
School of Pharmaceutical Sciences, The University of Nottingham, University Park, UK.
R A
RA
Quirk
W C
WC
Chan
M C
MC
Davies
S J
SJ
Tendler
K M
KM
Shakesheff
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002417:Cattle; D002448:Cell Adhesion; D002465:Cell Movement; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology; D019344:Lactic Acid / Q000737:chemistry; D008422:Materials Testing; D009842:Oligopeptides / Q000737:chemistry; D011091:Polyesters; D011107:Polylysine / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D013499:Surface Properties
D001672:Biocompatible Materials; D009842:Oligopeptides; D011091:Polyesters; D011108:Polymers; D011107:Polylysine; D019344:Lactic Acid; C033616:poly(lactide); C049554:glycyl-arginyl-glycyl-aspartyl-serine