Intradermal delivery of interleukin-12 plasmid DNA by in vivo electroporation.
Gene therapy depends on safe and efficient gene delivery. The skin is an attractive target for gene delivery because of its accessibility. Recently, in vivo electroporation has been shown to enhance expression after injection of plasmid DNA. In this study, we examined the use of electroporation to deliver plasmid DNA to cells of the skin in order to demonstrate that localized delivery can result in increased serum concentrations of a specific protein. Intradermal injection of a plasmid encoding luciferase resulted in low levels of expression. However, when injection was combined with electroporation, expression was significantly increased. When performing this procedure with a plasmid encoding interleukin-12, the induced serum concentrations of gamma-interferon were as much as 10 fold higher when electroporation was used. The results presented here demonstrate that electroporation can be used to augment the efficiency of direct injection of plasmid DNA to skin.
Heller
R
R
Department of Surgery, University of South Florida, Tampa, Florida 33612-4799, USA. rheller@hsc.usf.edu
Schultz
J
J
Lucas
M L
ML
Jaroszeski
M J
MJ
Heller
L C
LC
Gilbert
R A
RA
Moelling
K
K
Nicolau
C
C
D000818:Animals; D004247:DNA / Q000008:administration & dosage*; D018274:Electroporation / Q000379:methods*; D005069:Evaluation Studies as Topic; D005260:Female; D015316:Genetic Therapy; D007271:Injections, Intradermal; D018664:Interleukin-12 / Q000235:genetics*; D008156:Luciferases / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D010957:Plasmids
D018664:Interleukin-12; D004247:DNA; D008156:Luciferases