Complete genome comparison of porcine reproductive and respiratory syndrome virus parental and attenuated strains.
Two full-length porcine reproductive and respiratory syndrome virus (PRRSV) genomes, strain VR-2332 and its cell culture passaged descendent RespPRRS vaccine strain, were compared and analyzed in order to identify possible sites of attenuation. Of the 44 nucleotide changes, 13 resulted in conservative changes and 18 produced non-conservative changes. The results suggest that key amino acids in ORF1 may contribute to the phenotype of RespPRRS, which includes increased growth rate on MA-104 cells and decreased virulence in swine. The results provide a genetic basis for future manipulation of a PRRSV reverse genetics system.
Department of Veterinary PathoBiology, University of Minnesota, St. Paul, MN 55108, USA.
S
S
Yuan
D
D
Mickelson
M P
MP
Murtaugh
K S
KS
Faaberg
D020121:5' Untranslated Regions; D019943:Amino Acid Substitution; D000818:Animals; D001483:Base Sequence; D018929:Cell Culture Techniques; D004252:DNA Mutational Analysis; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009154:Mutation; D016366:Open Reading Frames / Q000235:genetics; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification / Q000472:pathogenicity; D012367:RNA, Viral; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017421:Sequence Analysis; D012692:Serial Passage; D013045:Species Specificity; D013552:Swine; D014613:Vaccines, Attenuated / Q000235:genetics / Q000302:isolation & purification; D015678:Viral Structural Proteins / Q000235:genetics; D014765:Viral Vaccines / Q000235:genetics* / Q000302:isolation & purification
D020121:5' Untranslated Regions; D012367:RNA, Viral; D014613:Vaccines, Attenuated; D015678:Viral Structural Proteins; D014765:Viral Vaccines