Strategies for prokaryotic expression of eukaryotic membrane proteins.
High-level heterologous expression of integral membrane proteins at full-length is a useful tool for their structural and functional characterization. Here, systems that have previously been used for efficient bacterial expression of eukaryotic membrane proteins are reviewed and novel vectors consisting of a modular fusion moiety based on nuclease A from Staphylococcus aureus are presented.
Laage
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Universität Heidelberg, Neurobiology Department, Im Neuenheimer Feld 364, 69120 Heidelberg, Germany.
Langosch
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D
D000595:Amino Acid Sequence; D000818:Animals; D001419:Bacteria / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D015870:Gene Expression; D005821:Genetic Techniques; D005822:Genetic Vectors; D015151:Immunoblotting; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D050683:R-SNARE Proteins; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics; D050600:SNARE Proteins; D033921:Vesicular Transport Proteins
D008565:Membrane Proteins; D050683:R-SNARE Proteins; D011993:Recombinant Fusion Proteins; D050600:SNARE Proteins; D033921:Vesicular Transport Proteins