Dot-immunobinding assay using indirect photoacoustic measurement.
Dot-immunobinding assay of hepatitis B surface antigen (HBsAg) was performed by a conventionally dot-ELISA technique with 4-chloro-1-naphthol staining, and the quantitative results were measured by an indirect photoacoustic method. In this method, a 2-mW helium-neon laser was applied to provide the excitation beam, the blue spots on the membrane were detected in a piezoelectric transducer-based photoacoustic cell. The operational conditions of measurement were optimized. A significant difference from the negative human serum was obtained for 50 pg in HBsAg detection. The method proposed provides a sensitive quantitative technique for dot-immunobinding assay.
Department of Chemistry, Wuhan University, China.
P
P
Zhou
Y
Y
Deng
Y
Y
Zeng
D004396:Coloring Agents; D006514:Hepatitis B Surface Antigens / Q000097:blood*; D006801:Humans; D015151:Immunoblotting / Q000295:instrumentation* / Q000379:methods / Q000592:standards*; D007834:Lasers; D009284:Naphthols; D012680:Sensitivity and Specificity
D004396:Coloring Agents; D006514:Hepatitis B Surface Antigens; D009284:Naphthols; C025917:4-chloro-1-naphthol