The ins and outs of nucleosome assembly.
De novo nucleosome assembly coupled to DNA replication and repair in vitro involves the histone chaperone chromatin assembly factor 1 (CAF-1). Recent studies support a model in which CAF-1 can be targeted to newly synthesized DNA through a direct interaction with proliferating cell nuclear antigen (PCNA) and can act synergistically with a newly identified histone chaperone. Insights have also been obtained into mechanisms by which this CAF-1-dependent pathway can establish a repressed chromatin state.
Institut Curie, Research section, UMR 218 du Centre National de la Recherche Scientifique (CNRS), 75248 Paris 05, Cedex, France. jill.mello@curie.fr
J A
JA
Mello
G
G
Almouzni
D000107:Acetylation; D000818:Animals; D056504:Chromatin Assembly Factor-1; D002868:Chromosomal Proteins, Non-Histone; D004247:DNA / Q000378:metabolism*; D004260:DNA Repair; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D008954:Models, Biological; D018832:Molecular Chaperones / Q000378:metabolism; D009707:Nucleosomes / Q000737:chemistry / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism
D056504:Chromatin Assembly Factor-1; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D006657:Histones; D018832:Molecular Chaperones; D009707:Nucleosomes; D018809:Proliferating Cell Nuclear Antigen; D004247:DNA