Thyrotropin-releasing hormone and its receptor in glia.
The presence of thyrotropin-releasing hormone (Thyroliberin, TRH) and its receptor (TRH-R) in frozen coronal sections of the adult rat spinal cord and neonatal rat astroglial cultures was investigated by means of immunocytochemistry and Western blot using polyclonal antibodies generated against the hormone and monoclonal antibodies originated against discrete sequences of the type 1 rat TRH receptor (TRH-R1). TRH-R1 and TRH are present both in astroglial cells from adult rats and in cultured cells from newborn animals. The localization of TRH and TRH-R1 in nonneuronal cells in the central nervous system may reflect that some of the neurotrophic actions of TRH upon the central nervous system are mediated by glial cells.
Departamento de Fisiología, Facultad de Medicina, Universidad de Alcalá, Campus Universitario, Carretera de Barcelona km. 33, 28871 Alcalá de Henares, Madrid, Spain. t.fernandez@cs.urjc.es
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Fernández-Agulló
D000818:Animals; D000911:Antibodies, Monoclonal; D001253:Astrocytes / Q000737:chemistry* / Q000166:cytology; D015153:Blotting, Western; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000737:chemistry / Q000166:cytology; D007150:Immunohistochemistry; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D018025:Receptors, Thyrotropin-Releasing Hormone / Q000032:analysis* / Q000276:immunology; D013116:Spinal Cord / Q000737:chemistry / Q000166:cytology; D013973:Thyrotropin-Releasing Hormone / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D018025:Receptors, Thyrotropin-Releasing Hormone; D013973:Thyrotropin-Releasing Hormone