Substrate selectivity of a 3-nitrophenol-induced metabolic system in Pseudomonas putida 2NP8 transforming nitroaromatic compounds into ammonia under aerobic conditions.
The 3-nitrophenol-induced enzyme system in cells of Pseudomonas putida 2NP8 manifested a wide substrate range in transforming nitroaromatic compounds through to ammonia production. All of the 30 mono- or dinitroaromatic substrates except 4-nitrophenol, 2,4-dinitrophenol, 2,4,6-trinitrophenol, 3-nitroaniline, 2-nitrobenzoic acid, and 2-nitrofuran were quickly transformed. Ammonia production from most nitroaromatic substrates appeared to be stoichiometric.
Department of Biology, University of Waterloo, Waterloo, Ontario, Canada.
J S
JS
Zhao
O P
OP
Ward
D000332:Aerobiosis; D000641:Ammonia / Q000378:metabolism*; D006841:Hydrocarbons, Aromatic / Q000737:chemistry / Q000378:metabolism; D009578:Nitrobenzenes / Q000737:chemistry / Q000378:metabolism*; D009596:Nitrophenols / Q000378:metabolism*; D009601:Nitroreductases / Q000378:metabolism*; D016958:Pseudomonas putida / Q000201:enzymology* / Q000254:growth & development; D013379:Substrate Specificity
D006841:Hydrocarbons, Aromatic; D009578:Nitrobenzenes; D009596:Nitrophenols; D000641:Ammonia; D009601:Nitroreductases; C049017:3-nitrophenol