[In vitro assessment of combining osteogenic cells with macroporous calcium-phosphate ceramics].
OBJECTIVE
Bone grafts or bone substitutes are required to fill bone defects resulting from trauma or surgical resection of tumors. Calcium-phosphate ceramics are synthetic bone substitutes which promote new bone formation by osteoconduction. These ceramics possess osteoconductive properties but have no intrinsic osteoinductive capacity. They are unable to induce new bone formation in extraossesous sites. One solution to develop bone substitutes with osteogenic properties would be to associate biomaterials with osteoprogenitors.


METHODS
We studied the in vitro osteogenic potential of human bone-marrow cells cultured on macroporous calcium phosphate (CaP) ceramic, examining stromal cell proliferation and differentiation. Osteogenic differentiation was evaluated in terms of alkaline phosphatase activity and immunological characterization of the extracellular fibrillar matrix formed by these cells. The specimens were examined by scanning and transmission electron microscopy.


RESULTS
Human bone-marrow cells proliferated on CaP ceramic. The proliferating bone-marrow cells expressed an osteoblastic phenotype as shown by alkaline phosphatase activity and synthesis in ceramic pores of an extracellular matrix composed of fibronectin, osteocalcin and collagen I. In addition, numerous microcrystals of apatite precipitated on the fibrillar matrix, producing a mineralized fibrillar network within the ceramic.


CONCLUSIONS
This study demonstrates that human bone-marrow cells cultured on macroporous CaP ceramic do not lose their osteoblastic phenotype even after 21 days of culture, and that they can induce osteogenesis in a CaP ceramic in vitro. This type of new "hybrid material" appears promising for the future.
Heymann
D
D
EE 99-01, Laboratoire de Physiopathologie de la Résorption Osseuse, Nantes, France. dominique.heymann@sante.univ-nantes.fr
Delécrin
J
J
Deschamps
C
C
Gouin
F
F
Padrines
M
M
Passuti
N
N
D001854:Bone Marrow Cells / Q000502:physiology* / Q000648:ultrastructure; D018786:Bone Substitutes / Q000627:therapeutic use*; D002130:Calcium Phosphates / Q000627:therapeutic use*; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division; D004353:Drug Evaluation, Preclinical; D006801:Humans; D006882:Hydroxyapatites / Q000627:therapeutic use*; D010006:Osteoblasts / Q000187:drug effects* / Q000502:physiology* / Q000648:ultrastructure; D010012:Osteogenesis / Q000502:physiology*
D018786:Bone Substitutes; D002130:Calcium Phosphates; D006882:Hydroxyapatites; C094280:hydroxyapatite-tricalciumphosphate composite