Capillary electrophoretic study of carboplatin and analogues with nucleoside monophosphates, di- and trinucleotides.
Carboplatin (cis-diammine-1,1-cyclobutane-dicarboxylatoplatinum(II)) is the only cisplatin (cis-diammine-dichloroplatinum(II)) derivative currently available for the treatment of cancer worldwide. The higher stability of the carboxylate ligand compared to the coordinated chloride in cisplatin results in a reduced reactivity of the molecule. Capillary electrophoresis has been applied for investigating the adduct formation of carboplatin and analogues with nucleoside monophosphates, di- and trinucleotides. Adduct formation results in a significant shift of the absorption maximum to lower energy compared to free nucleotides. Therefore, characterization of the analytes was performed by UV additionally to NMR spectroscopy. A preference for GMP- and AMP-coordination was found. The ability of separating all four common nucleotides and their major platinum adducts in a single run demonstrates the suitability of CE for this kind of investigations.
Institute of Inorganic Chemistry, University of Vienna, Austria.
A
A
Küng
A
A
Zenker
Mathea S
MS
Galanski
B K
BK
Keppler
D000249:Adenosine Monophosphate / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000737:chemistry*; D016190:Carboplatin / Q000737:chemistry*; D003568:Cytidine Monophosphate / Q000737:chemistry*; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D019075:Electrophoresis, Capillary / Q000379:methods*; D006157:Guanosine Monophosphate / Q000737:chemistry*; D006801:Humans; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D009841:Oligonucleotides / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013938:Thymidine Monophosphate / Q000737:chemistry*
D000970:Antineoplastic Agents; D008024:Ligands; D009841:Oligonucleotides; D013938:Thymidine Monophosphate; D000249:Adenosine Monophosphate; D006157:Guanosine Monophosphate; D004247:DNA; D016190:Carboplatin; D003568:Cytidine Monophosphate