[Analysis of surface markers of long-term cultured human umbilical cord blood hemopoietic cells].
OBJECTIVE
To explore the optimal time for in vitro expansion and transplantation of umbilical cord blood hemopoietic cells.


METHODS
Hemopoietic cells from human umbilical cord blood were cultured with SCF, rIL-3, rIL-6 and rIL-1 beta in a long term culture system and changes of cellular surface markers were observed.


RESULTS
CD3+ and CD20+ lymphocytes increased at the 7th day, and gradually decreased to the lowest level after the 14th day. CD34 positive cells increased after the 7th day, and reached the highest level at the 14th day, then decreased gradually but maintained at a higher level than that at the 7th day. CD33 positive cells increased at the 7th day, and reached the highest level from the 14th to the 21st day, then decreased after the 28th day. CD42b positive cells began to increase at the 21st day, reached the peak at the 28th day, and still maintained higher level at the 35th day. HLA-DR positive cells increased from the 14th to the 21st day.


CONCLUSIONS
The culture system containing the four growth factors is able to expand hemopoietic cells from human umbilical cord blood and keeps them intact. From the 14th to the 21st day, the number of hemopoietic cells reached the highest level, and is suitable for transplantation.
Wen
Z
Z
Jiangxi Institute of Medical Sciences, Nanchang 330006.
Dai
Y
Y
Hu
A
A
D018951:Antigens, CD20 / Q000378:metabolism; D018952:Antigens, CD34 / Q000378:metabolism; D015415:Biomarkers; D017252:CD3 Complex / Q000378:metabolism*; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007231:Infant, Newborn
D018951:Antigens, CD20; D018952:Antigens, CD34; D015415:Biomarkers; D017252:CD3 Complex