Regulation of the CCAAT-Binding NF-Y subunits in Arabidopsis thaliana.
NF-Y is a CCAAT-specific binding factor composed of three distinct subunits. In vertebrates and fungi all three subunits are encoded by evolutionary conserved single copy genes. In this report we have cloned twenty-three NF-Y genes in A. thaliana, assessed their mRNA expression levels in a large number of tissues and confirmed that indeed multiple CCAAT-binding activities are present. Alignments of the genes coding for the three NF-Y subunits yield a considerable amount of information concerning the divergence/conservation of protein subdomains and of single residues within the conserved parts. Careful evaluation of mRNA expression levels by sensitive RT-PCR assays provide evidence that all three subunits have members that are ubiquitous and others that are tissue-specific and induced only after the switch to reproductive growth phase, in flowers and siliques.
Gusmaroli
G
G
Dipartimento di Genetica e Biologia dei Microrganismi, Università degli Studi di Milano, Via Celoria 26, 20133 Milan, Italy.
Tonelli
C
C
Mantovani
R
R
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics*; D023081:CCAAT-Binding Factor / Q000235:genetics*; D003001:Cloning, Molecular; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics; D020033:Protein Isoforms / Q000235:genetics; D021122:Protein Subunits; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D023081:CCAAT-Binding Factor; D010940:Plant Proteins; D020033:Protein Isoforms; D021122:Protein Subunits; D018749:RNA, Plant