A short peptide derived from the antisense homology box of Fas ligand induces apoptosis in anti-Fas antibody-insensitive human ovarian cancer cells.
We found that a short synthetic peptide corresponding to the "antisense homology box" of Fas ligand induced apoptotic cell death of Fas-expressing human ovarian cancer cell lines. The peptide was deduced from residues 256-265 of human Fas ligand, based on the hypothesis that it should contain a specific binding site to the corresponding Fas. Interestingly, the ovarian cancer cell line NOS4, which was sensitive to anti-Fas antibody induced apoptosis, was not affected by the peptide, whereas another cell line, SKOV-3, which was insensitive to anti-Fas antibody, was killed by the peptide. Thus, this short peptide was shown to have a unique activity to induce apoptosis in human ovarian cancer cells in a manner different from anti-Fas antibody.
Hayakawa
A
A
Department of Equipment for Research and Education, Nagoya University, School of Medicine, Japan.
Kojima
T
T
Yokoyama
I
I
Suzuki
H
H
Tajiri
H
H
Nakashima
I
I
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D058846:Antibodies, Monoclonal, Murine-Derived; D016375:Antisense Elements (Genetics); D017209:Apoptosis; D053938:DNA Fragmentation; D053222:Fas Ligand Protein; D005260:Female; D006801:Humans; D008562:Membrane Glycoproteins / Q000494:pharmacology*; D009707:Nucleosomes; D009842:Oligopeptides / Q000494:pharmacology*; D010051:Ovarian Neoplasms / Q000188:drug therapy*; D010446:Peptide Fragments / Q000494:pharmacology*; D017386:Sequence Homology, Amino Acid; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D058846:Antibodies, Monoclonal, Murine-Derived; D016375:Antisense Elements (Genetics); C505661:FASLG protein, human; D053222:Fas Ligand Protein; D008562:Membrane Glycoproteins; D009707:Nucleosomes; D009842:Oligopeptides; D010446:Peptide Fragments; C411023:anti-Fas monoclonal antibody