[Sulforodamine 101: novel effective marker in liposomal immunoassay].
The new fluorescence marker Sulforodamine 101 that allows one to avoid a labour-consuming stage of purification of a marker substance (for example, calceine, other fluorescein derivatives) from hydrophobic admixtures that reduce the storage of a liposomal reagent was used in liposomal immunoassay. Sulforodamine 101 shows the maximum increase in a fluorescence signal when liposomes are destroyed with encapsulated marker, which is 45 times greater than does Sulforodamine B (8 times) and comparable with calceine (30 times). The comparative studies using liposomes containing calceine and Sulforodamine 101 have indicated that they are similar storage and sensitivity (50 ng/ml) of liposomal test system as determined by the concentration of F. tularensis polysaccharides in solutions.
Skopinskaia
S N
SN
Iarkov
S P
SP
Khramov
E N
EN
Zlobin
V N
VN
D015415:Biomarkers / Q000032:analysis; D004396:Coloring Agents / Q000032:analysis; D005452:Fluoresceins / Q000032:analysis; D005456:Fluorescent Dyes / Q000032:analysis; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008081:Liposomes / Q000737:chemistry*; D012235:Rhodamines / Q000032:analysis
D015415:Biomarkers; D004396:Coloring Agents; D005452:Fluoresceins; D005456:Fluorescent Dyes; D008081:Liposomes; D012235:Rhodamines; C050829:sulforhodamine 101; C007740:fluorexon