Application of magdala red as a fluorescence probe in the determination of nucleic acids.
A fluorescence quenching method was developed for the rapid determination of DNA and RNA using magdala red as fluorescence probe. In weakly acidic medium, the fluorescence of magdala red (lambdaex/lambdaem = 540/555 nm) can be largely quenched by DNA or RNA. The calibration graphs are linear over the range 0.01-1.2 microg/mL for both calf thymus DNA (CT DNA) and salmon DNA (SM DNA), and 0.015-1.0 microg/mL for yeast RNA, respectively. The corresponding detection limits are 6.0 ng/mL for CT DNA, 7.0 ng/mL for SM DNA and 15.0 ng/mL for yeast RNA, respectively. CT DNA could be determined in the presence of 20% (w/w) yeast RNA, and the relative standard deviation of six replicate measurements is 3.18% for 400 ng/mL of CT DNA. Interference from coexisting substances in the determination of DNA was also examined. Real samples were determined with satisfactory results.
Yang
H H
HH
Department of Chemistry, Xiamen University, P.R. China.
Zhu
Q Z
QZ
Chen
Q Y
QY
Li
D H
DH
Xu
J G
JG
D000225:Adenine; D000818:Animals; D002417:Cattle; D003596:Cytosine; D004247:DNA / Q000032:analysis*; D005456:Fluorescent Dyes; D006147:Guanine; D007202:Indicators and Reagents; D009281:Naphthalenes; D011506:Proteins; D011719:Pyrazines; D012313:RNA / Q000032:analysis*; D012331:RNA, Fungal / Q000032:analysis; D015203:Reproducibility of Results; D012441:Saccharomyces cerevisiae; D012474:Salmon; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D013941:Thymine; D013950:Thymus Gland
D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D009281:Naphthalenes; D011506:Proteins; D011719:Pyrazines; D012331:RNA, Fungal; C412337:magdala red; D006147:Guanine; D012313:RNA; D003596:Cytosine; D004247:DNA; D000225:Adenine; D013941:Thymine