Determination of 4-hydroxytamoxifen in mouse plasma in the pg/mL range by gradient capillary liquid chromatography/tandem mass spectrometry.
Capillary high-performance liquid chromatography (HPLC; 300 microm i.d.) coupled to tandem mass spectrometry has been used to determine the concentration of 4-hydroxytamoxifen in mouse plasma in the pg/mL range following the administration of Tamoxifen. A limit of quantification (LOQ) of 100 pg/mL was achieved using only 25 microL of plasma. The on-column sensitivity was determined to be 100 fg. The column performance was determined isocratically before and after the assay and showed only a 15% reduction in performance after 70 injections of plasma extract. No significant peak band broadening was observed due to the mass spectrometer interface using a standard TurboIonspray source.
Glaxo Wellcome Research and Development, BioMet Division, Park Road, Ware, Herts SG12 ODP, UK.
R S
RS
Plumb
H
H
Warwick
D
D
Higton
G J
GJ
Dear
D N
DN
Mallett
D000284:Administration, Oral; D000818:Animals; D002138:Calibration; D002197:Capillary Action; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D051379:Mice; D012680:Sensitivity and Specificity; D013629:Tamoxifen / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood* / Q000493:pharmacokinetics*
D013629:Tamoxifen; C016601:afimoxifene