Visualizing filamentous actin on lipid bilayers by atomic force microscopy in solution.
The surface structure of actin filaments (F-actin) was visualized at high resolution, by atomic force microscopy (AFM) in aqueous solution, in large paracrystals prepared on positively charged lipid monolayers. The increased stability of these closely packed specimens allowed us to show that both the long pitch (38 nm) and the monomer (5.8 nm) can be directly resolved by AFM in the contact mode. The right-handed helical surface, distinguishable in high resolution images, was compared with reconstructed models based on electron microscopy. The height of the rafts, a measure of the actin filament diameter, was 10 +/- 1 nm, whereas the smaller inter-filament distance, 8 +/- 1 nm, was consistent with interdigitation of the filaments. The 10 +/- 1 nm F-actin diameter is in good agreement with the results of fibre X-ray diffraction. As such specimens are relatively easy to prepare without specialized equipment, this method may allow the study of the thin filaments in which F-actin-associated proteins are also present.
Department of Molecular Physiology & Biological Physics, University of Virginia School of Medicine, Box 800736, Charlottesville, VA 22908-0736, U.S.A.
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D008841:Actin Cytoskeleton / Q000648:ultrastructure*; D003460:Crystallization; D008051:Lipid Bilayers; D018625:Microscopy, Atomic Force / Q000379:methods*; D012996:Solutions
D008051:Lipid Bilayers; D012996:Solutions