A simple method for preparation of immuno-magnetic liposomes.
A simple and readily manoeuverable method for preparing immuno-magnetic liposomes that indigenously contain binding sites for attaching other molecules like antibodies on their exterior surface is described. In this method magnetic unilamellar vesicles are prepared from a mixture of phosphatidylcholine, cholesterol, small amounts of a linear chain aldehyde and colloidal particles of magnetic iron oxide, using a reverse phase evaporation technique. The aldehyde (dedecanal) molecules align themselves among the lipid molecules in the bilayer with their aldehyde groups exposed to the aqueous phase, allowing straight attachment of antibody molecules (human-antimouse IgG-FITC in this case) in one single step. The success of this approach is confirmed by fluorescence microscopy as well as binding of the resulting immuno-magnetic liposomes to their corresponding target cells.
Elmi
M M
MM
Institute of Biochemistry and Biophysics, Tehran University, PO Box 13145-1384, Tehran, Iran.
Sarbolouki
M N
MN
D000447:Aldehydes / Q000737:chemistry; D000906:Antibodies / Q000737:chemistry; D002234:Carbodiimides / Q000737:chemistry; D004339:Drug Compounding; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D005976:Glutaral / Q000737:chemistry; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D066298:In Vitro Techniques; D008081:Liposomes / Q000138:chemical synthesis*; D008214:Lymphocytes / Q000276:immunology / Q000648:ultrastructure; D010316:Particle Size; D013056:Spectrophotometry, Ultraviolet
D000447:Aldehydes; D000906:Antibodies; D002234:Carbodiimides; D005456:Fluorescent Dyes; D008081:Liposomes; D016650:Fluorescein-5-isothiocyanate; D005976:Glutaral