Evaluation of avian-specific probiotic and Salmonella enteritidis-, Salmonella typhimurium-, and Salmonella heidelberg-specific antibodies on cecal colonization and organ invasion of Salmonella enteritidis in broilers.
Salmonella Enteritidis colonizes the intestinal tract of poultry and causes foodborne illness in humans. Reduction of Salmonella Enteritidis colonization in the intestinal tract of poultry reduces potential carcass contamination during slaughter. The purpose of this study was to determine the effect of an avian-specific probiotic combined with Salmonella Enteritidis-, Salmonella Typhimurium-, and Salmonella Heidelberg-specific antibodies on the cecal colonization and organ invasion of Salmonella Enteritidis in broiler as well as on body weights. The treatment group was defined as chicks spray-vaccinated with Avian Pac Plus at the hatchery and given Avian Pac Plus for the first 3 days after placement. An intermediate treatment was given at 10 and 14 days, 2 days prior to vaccination and 2 days postvaccination. All birds were vaccinated with Newcastle disease vaccine, La Sota virus (one drop/eye) at 12 days of age. A final treatment was given 3 days preslaughter. The control group was defined as chicks not given Avian Pac Plus at any time. Six hours after oral administration of the probiotic suspension (treatment group) or water (control group) at placement, the chicks were challenged with Salmonella Enteritidis. All chickens were orally inoculated with 0.25 ml of Salmonella Enteritidis that contained 4 x 10(7) CFU/1.0 ml. Cecal colonization and organ invasion were evaluated for Salmonella Enteritidis on days 0, 1, 3, 7, 10, 17, 24, 31, 38, and 41. The probiotic-treated group had a significantly lower concentration of Salmonella Enteritidis cecal colonization at days 3, 7, 10, 17, 24, 31, 38, and 41 when compared to the nontreated, control group (P < 0.05). Similarly, there was a significant difference (P < 0.05) in the isolation of Salmonella Enteritidis from the internal organs (liver and spleen) when probiotic-treated and nonprobiotic-treated groups were compared. There was no significant difference (P > 0.05) in the mean body weight between the two experimental groups at each collection period. These results indicated that a combination of Lactobacillus acidophilus, Streptococcus faecium, and Salmonella Enteritidis, Salmonella Typhimurium, and Salmonella Heidelberg-Specific antibodies have a beneficial effect in reducing the colonization of Salmonella Enteritidis in market-aged broilers.
Tellez
G
G
Departamento de Produccion Animal Aves, Facultad de Medicina Veterinaria y Zootecnia, UNAM Mexico DF. isaias@servidor.unam.mx
Petrone
V M
VM
Escorcia
M
M
Morishita
T Y
TY
Cobb
C W
CW
Villaseñor
L
L
Promsopone
B
B
D000818:Animals; D000907:Antibodies, Bacterial / Q000008:administration & dosage*; D001835:Body Weight; D002432:Cecum / Q000382:microbiology; D002645:Chickens / Q000382:microbiology*; D015169:Colony Count, Microbial; D005766:Gastrointestinal Contents / Q000382:microbiology; D007116:Immunization, Passive; D008099:Liver / Q000382:microbiology; D011201:Poultry Diseases / Q000382:microbiology / Q000517:prevention & control*; D019936:Probiotics / Q000008:administration & dosage*; D012475:Salmonella / Q000276:immunology*; D012481:Salmonella Infections, Animal / Q000382:microbiology / Q000517:prevention & control*; D012477:Salmonella enteritidis / Q000254:growth & development* / Q000276:immunology / Q000472:pathogenicity; D013154:Spleen / Q000382:microbiology; D013997:Time Factors
D000907:Antibodies, Bacterial