[Zymographic analysis of MMPs in human carcinoma cell lines with different metastasis potential].
OBJECTIVE
To investigate the correlation between matrix metalloproteinases (MMPs) activities and metastatic potential of several groups of human carcinoma cells with different metastatic potential.


METHODS
Several groups of human carcinoma cell lines (human lung carcinoma, human prostatic carcinoma and melanoma) with different metastatic potential were selected. By using cell culture, collection and concentration of conditioned media and zymographic analysis method the difference of MMPs production and activity among those cell lines were detected.


RESULTS
The MMPs production capabilities of carcinoma cells rose following the increase of their invasive and metastatic potentials: that of PG is much higher than PAa's, and BE1 is higher also than CL3 and LH7. Advanced stage melanoma cell WM983a and WM451 product MMP-9, but primary stage cells don't. There are MMP-9 in the conditioned medium of prostatic carcinoma cell PC-3M, the metastatic clone of PC-3 which don't express MMP-9.


CONCLUSIONS
The expression of MMPs especially MMP-9 of carcinoma cells is closely correlated to the metastatic and invasive potential.
Department of Pathology, Beijing Medical University, Beijing 100083.
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H
Li
W
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Fang
J
J
Zheng
D000230:Adenocarcinoma / Q000201:enzymology / Q000556:secondary; D000818:Animals; D018286:Carcinoma, Giant Cell / Q000201:enzymology / Q000556:secondary*; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology* / Q000473:pathology*; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D008545:Melanoma / Q000201:enzymology / Q000556:secondary; D051379:Mice; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D011471:Prostatic Neoplasms / Q000201:enzymology / Q000473:pathology; D014407:Tumor Cells, Cultured
D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9