Characterization of the mac-1 gene encoding a putative ABC transporter from Myxococcus xanthus.
The mac-1 gene of Myxococcus xanthus TA, an antibiotic TA producer, encoded a protein with strong sequence similarity to the antibiotic ATP-binding cassette (ABC) transporter for macrolide antibiotics. The mac-1 gene encoding protein (Mac-1) had two ATP-binding domains containing Walker A and B motifs, and no hydrophobic transmembrane regions. Insertional inactivation of mac-1 caused enhanced sensitivity to oleandomycin, a macrolide antibiotic, while the mac-1 mutant showed normal export of antibiotic TA into the extracellular fluid. The mac-1 mutant could form mounds, but was unable to form fruiting bodies or sporulate under nutrient starvation. A primary role for Mac-1 in M. xanthus may be as a transporter which exports or imports a molecule required for the sporulation process.
Kimura
Y
Y
Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa 761-0795, Japan. kimura@ag.kagawa-u.ac.jp
Yamanishi
Y
Y
Tokumasu
Y
Y
Terasaka
H
H
Yoshinobu
J
J
D018528:ATP-Binding Cassette Transporters / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D001692:Biological Transport; D003001:Cloning, Molecular; D005798:Genes, Bacterial / Q000235:genetics; D018942:Macrolides; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D016941:Myxococcus xanthus / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D009827:Oleandomycin / Q000494:pharmacology; D017354:Point Mutation / Q000235:genetics; D017434:Protein Structure, Tertiary; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D013171:Spores, Bacterial / Q000502:physiology; D013329:Structure-Activity Relationship
D018528:ATP-Binding Cassette Transporters; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D018942:Macrolides; D012329:RNA, Bacterial; D012333:RNA, Messenger; C035536:Myxococcus xanthus antibiotic TA; D000255:Adenosine Triphosphate; D009827:Oleandomycin