Different susceptibility to oxidation of proline and arginine residues of apolipoprotein B-100 among subspecies of low density lipoproteins.
gamma-Glutamyl semialdehyde is a primary oxidation product of apolipoprotein (apo) B-100 proline (Pro) and arginine (Arg) side chain residues. By reduction gamma-glutamyl semialdehyde forms 5-hydroxy-2-aminovaleric acid (HAVA). Here we describe the application of sensitive and specific HAVA measurement to characterize the formation of gamma-glutamyl semialdehyde in several domains of apoB-100 in LDL(1) (S(f) 7-12) and LDL(2) (S(f) 0-7) subfractions subjected to oxidative damage in the presence of iron in vitro. Results suggest that susceptibility of apoB-100 Pro and Arg residues toward oxygen radicals drastically changes along the lipoprotein metabolic cascade.
Institute and Policlinic of Clinical Metabolic Research, Medical Faculty Carl Gustav Carus, Technical University, Fetscherstrasse 74, D-01307 Dresden, Germany. jens.pietzsch@mailbox.tu-dresden.de
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Julius
D000328:Adult; D000596:Amino Acids / Q000737:chemistry; D053299:Apolipoprotein B-100; D001055:Apolipoproteins B / Q000097:blood / Q000737:chemistry*; D001120:Arginine / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D005971:Glutamates / Q000737:chemistry; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood / Q000737:chemistry*; D008297:Male; D010084:Oxidation-Reduction; D011392:Proline / Q000737:chemistry*; D017382:Reactive Oxygen Species
D000596:Amino Acids; D053299:Apolipoprotein B-100; D001055:Apolipoproteins B; D005971:Glutamates; D008077:Lipoproteins, LDL; D017382:Reactive Oxygen Species; C021199:glutamic acid gamma-semialdehyde; C004387:2-amino-5-hydroxyvaleric acid; D001120:Arginine; D011392:Proline