Extended photoreceptor viability by light stress in the RCS rats but not in the opsin P23H mutant rats.
OBJECTIVE
To determine the effect of light stress on retinal function and long-term photoreceptor viability in Royal College of Surgeons (RCS) rats and the applicability of the light treatment to the opsin P23H mutant rats.


METHODS
RCS rats at postnatal day (P)23 were illuminated with 120 foot-candles (fc) white light for 10 hours. Photoreceptor survival and basic fibroblast growth factor (bFGF) expression were measured at P60 and P83. Retinal function was evaluated by electroretinography. Opsin P23H transgenic rats were treated with light at P28 and analyzed at P70 for photoreceptor viability, ultrastructure, and bFGF expression.


RESULTS
Light-treated RCS rats at P60 had four to five rows of nuclei versus one to two rows in untreated littermates. The average amplitude of the ERG b-wave was 28 microV in treated rats, compared with 6 microV in untreated littermates. By P83 there was still significant preservation of the ONL in treated rats. Immunoblot analysis showed a high expression of bFGF in the treated retinas even 2 months after treatment. Illumination of P23H rats at P28 with 120 fc white light for 10 hours caused substantial photoreceptor cell death, although bFGF expression was upregulated. Lowered illumination dosages continued to cause photoreceptor damage until levels were reached that neither caused damage nor enhanced survival.


CONCLUSIONS
Although light stress promotes photoreceptor survival and function in the RCS rat, it elicits death signals in the P23H rats that may not be overcome by survival-promoting factors. Therefore, use of light stress to promote photoreceptor survival should be considered with regard to sensitivity of the mutation to light damage.
Department of Pharmacology, The University of Texas Health Science Center at San Antonio, TX, USA.
I
I
Nir
J M
JM
Harrison
C
C
Liu
R
R
Wen
D000818:Animals; D030801:Animals, Genetically Modified; D002470:Cell Survival; D004591:Electrophoresis, Polyacrylamide Gel; D004596:Electroretinography; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D015151:Immunoblotting; D008027:Light; D009154:Mutation; D020419:Photoreceptor Cells, Vertebrate / Q000378:metabolism / Q000528:radiation effects* / Q000648:ultrastructure; D011833:Radiation Injuries, Experimental / Q000235:genetics / Q000473:pathology / Q000517:prevention & control*; D051381:Rats; D011922:Rats, Mutant Strains; D012162:Retinal Degeneration / Q000235:genetics / Q000473:pathology / Q000517:prevention & control*; D017299:Rod Opsins / Q000235:genetics* / Q000378:metabolism; D013312:Stress, Physiological / Q000517:prevention & control*
D017299:Rod Opsins; D016222:Fibroblast Growth Factor 2