[Use of PCR for the identification of representatives of Lactobacillus and Streptococcus].
REP- and ERIC-PCR genome analysis of lactic acid bacteria of genera Lactobacillus and Streptococcus have shown the presence of REP- and ERIC-repetitive sequences with high degree of homology. Amplification products which separation in agar gel results in formation of a specific fingerprint are obtained under REP- and ERIC-PCR. It is shown that REP- and ERIC-specific primers can be used for PCR identification of both enterobacteria and lactic acid bacteria isolated from different ecological niches.
Kovalenko
N K
NK
Institute of Microbiology and Virology, Ukrainian National Academy of Sciences, 154 Zabolotny St., Kyiv, 03143, Ukraine.
Bur'ianovskiĭ
L N
LN
Podgorskiĭ
V S
VS
D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid; D013291:Streptococcus / Q000145:classification* / Q000235:genetics
D004269:DNA, Bacterial