[Correlation between methylation on SmaI locus of the CDKN2/p16 gene CpG island and lung cancer].
OBJECTIVE
To study the relationship between the status of methylation of the CDKN2/p16 gene 5'-CpG island and lung cancer.


METHODS
Genomic DNA was digested with methylation-sensitive enzyme SmaI and Southern blotting was applied to analyze the status of methylation of the CDKN2/p16 in 89 cases of lung cancer and 10 cases of normal lung tissue.


RESULTS
Of the 89 cases of lung cancer studied, the CDKN2/p16 gene was shown to be methylated in 15 cases (16.9%). It occurred in 12 of 42 p16 protein negative cases (28.6%), and in 3 of 47 p16 protein positive cases. The CDKN2/p16 gene was not shown to be methylated in 10 cases of normal lung tissue.


CONCLUSIONS
Methylation of the CDKN2/p16 gene 5'-CpG island may be an important mechanism for gene inactivation in lung cancer progression.
Cancer Center of PLA, Nanjing 81 Hospital, Nanjing 210002, China.
C
C
Su
Y
Y
Ye
D
D
Wang
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D018899:CpG Islands / Q000235:genetics*; D004262:DNA Restriction Enzymes; D005260:Female; D020868:Gene Silencing; D019942:Genes, p16; D006801:Humans; D006824:Hybridization, Genetic; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D008745:Methylation; D008875:Middle Aged
D004262:DNA Restriction Enzymes