Negative energy balance does not decrease expression of leukocyte adhesion or antigen-presenting molecules in cattle.
Sixteen yearling Holstein steers were fed for 210 or 60% of maintenance requirements to impose positive or negative energy balance, respectively. Blood was collected and analyzed for serum concentration of nonesterified fatty acids (NEFA), and leukocytes were isolated and counted. Isolated leukocytes were then analyzed for expression of the adhesion molecules L-selectin (CD62L), Mac-1 (CD11b and CD18), and major histocompatability complex (MHC) class I and class II molecules with immunostaining and flow cytometric analysis. Negative energy balance increased the concentration of NEFA in serum (P < 0.0001). Expression of CD62L on neutrophils was increased 14% during negative energy balance (P = 0.03). Energy balance did not affect expression of CD62L on any other cell types or expression of CD11b or CD18. Negative energy balance did not affect MHC class I expression but resulted in a small but significant increase in the expression of MHC class II (P = 0.03). The results of this study provide little evidence that nutritionally created negative energy balance impairs expression of CD62L, CD11b, and CD18 or expression of MHC class I or MHC class II molecules by resting bovine blood leukocytes.
Perkins
K H
KH
Department of Animal Science, Michigan State University, East Lansing 48824, USA.
VandeHaar
M J
MJ
Tempelman
R J
RJ
Burton
J L
JL
D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D018821:CD18 Antigens / Q000032:analysis; D002417:Cattle / Q000097:blood*; D004032:Diet; D004734:Energy Metabolism; D005230:Fatty Acids, Nonesterified / Q000032:analysis / Q000097:blood*; D005434:Flow Cytometry; D015870:Gene Expression; D007150:Immunohistochemistry; D007962:Leukocytes / Q000276:immunology*; D016177:Macrophage-1 Antigen / Q000032:analysis; D008285:Major Histocompatibility Complex; D008297:Male; D015233:Models, Statistical
D018821:CD18 Antigens; D005230:Fatty Acids, Nonesterified; D016177:Macrophage-1 Antigen