Liquid chromatographic method with electrochemical detection for determination of cisapride in serum.
A sensitive liquid chromatographic (LC) method using electrochemical detection was developed for the identification and quantitation of cisapride in serum. The serum samples were deproteinized by a simple acetonitrile precipitation technique followed by n-hexane extraction. Cisapride in the deproteinized serum was separated by an isocratic elution with an ODS Hypersil LC column (150 x 4.6 mm) using a mobile phase consisting of 0.05M Na2HPO4-acetonitrile (60 + 40), pH 8.4. Cisapride eluted from the column was detected by a Coulochem II electrochemical detector. The precision of this assay method was determined by intra- and inter-day analyses of cisapride-free fetal bovine serum samples that were spiked with 25, 50, and 100 ng/mL cisapride. For the intra-day assay, recoveries were 94.3 +/- 1.4, 90.1 +/- 2.9, and 103.2 +/- 9.2%, respectively. This electrochemical detection LC method could be very useful in monitoring plasma levels of cisapride.
Central University of Venezuela, College of Pharmacy, Caracas.
M C
MC
de Condado
A
A
Malave
M A
MA
Dorantes-Hernandez
A
A
Rathinavelu
D002853:Chromatography, Liquid; D020117:Cisapride / Q000097:blood*; D005765:Gastrointestinal Agents / Q000097:blood*; D006801:Humans; D007202:Indicators and Reagents; D015203:Reproducibility of Results; D018490:Serotonin Agents / Q000097:blood*; D013056:Spectrophotometry, Ultraviolet
D005765:Gastrointestinal Agents; D007202:Indicators and Reagents; D018490:Serotonin Agents; D020117:Cisapride