Accumulation of barley stripe mosaic virus is significantly reduced in transgenic wheat plants expressing a bacterial ribonuclease.
An rnc70 gene encoding a mutant bacterial ribonuclease III (RNase III) was introduced into wheat (Triticum aestivum cv. Bobwhite) by microprojectile bombardment. T1, T2, and T3 plants regenerated from three transgenic callus lines were challenged with barley stripe mosaic virus. Plants expressing RNase III exhibited a high level of resistance to the virus infection. This resistance was evidenced by the absence of virus symptoms and reduced accumulation of virions in these plants. The result demonstrates that this pathogen-targeted resistance strategy can be effectively employed in conferring resistance to viral diseases of cereal crops.
Department of Plant Pathology and Center for Biotechnology, University of Nebraska, Lincoln 68583-0722, USA.
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Zhang
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French
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Langenberg
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Mitra
D015153:Blotting, Western; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D004722:Endoribonucleases / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D001467:Hordeum / Q000821:virology*; D009029:Mosaic Viruses / Q000235:genetics / Q000502:physiology*; D009154:Mutation; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D043244:Ribonuclease III; D014170:Transformation, Genetic; D014908:Triticum / Q000201:enzymology / Q000821:virology*; D014779:Virus Replication
D017931:DNA Primers; D012367:RNA, Viral; D004722:Endoribonucleases; D043244:Ribonuclease III