[New full length cDNA cloned from normal pituitary and pituitary tumors].
OBJECTIVE
To clone new full length cDNA from normal pituitary and pituitary tumors.


METHODS
cDNA libraries of human normal pituitary and pituitary tumors were constructed, and large-scale sequencing and bioinformatics were used. Silico cloning and rapid amplification of cDNA ends (RACE) were also used.


RESULTS
67 new full length cDNAs were cloned: 41 were cloned from normal pituitary, and 26 from pituitary tumors. Of the 67 cDNAs, 16 were related with endocrine function; at least 2 with a classical signal peptide were secretory protein, and 3 with classical transmembrane domains were transmembrane protein. Among the 5 cloned new full length cDNAs involved in signal transduction pathway of hormone, at least 2 participated in signal transduction pathway of receptors with tyrosine kinase activition.


CONCLUSIONS
During short time, 67 new full length cDNA were cloned from normal pituitary and pituitary tumors by large-scale producing expressed sequence tags. Some of them may play an important role in the regulation of physiological and pathological processes of pituitary.
Shanghai Institute of Endocrinology & Shanghai Institute of Hematology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025 China.
H
H
Song
Y
Y
Peng
R
R
Hu
D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D030561:Databases, Nucleic Acid; D006801:Humans; D010902:Pituitary Gland / Q000502:physiology*; D010911:Pituitary Neoplasms / Q000235:genetics*
D018076:DNA, Complementary