Activation of alveolar phospholipase A2 after hydrochloric acid aspiration in rats.
OBJECTIVE
The present study was carried out to determine phospholipase A2 (PLA2) activity in the bronchoalveolar lavage fluid (BALF) in rats subjected to HCI aspiration.


METHODS
Rats were allocated into one of five groups. Groups H-1 and H-3 received instillation of HCI into lungs. Groups S-1 and S-3 received saline instead of HCI. Group C received no instillation. BAL was performed according to the protocol, that is, 1 hour after the instillation in groups H-1 and S-1, 3 hours after the instillation in groups H-3 and S-3, and arbitrarily in group C. Obtain BALF was analyzed for the protein concentration, PLA2 activity, and the molecular mass of PLA2.


RESULTS
The protein concentration in BALF showed an increase in groups H-1 and H-3. PLA2 activity decreased in group H-1, but increased in group H-3, compared with groups S-1 and S-3, respectively. PLA2 in groups C and H-1 revealed a high molecular mass (HM), but that in group H-3 revealed a low molecular mass (LM).


CONCLUSIONS
There is an increase in the alveolar LM-PLA2 at inflammatory phase after HCI aspiration, suggesting the pathophysiologic role of LM-PLA2 in the acute lung injury.
Department of Anesthesiology, Nagasaki University School of Medicine, Japan.
Y
Y
Terao
S
S
Haseba
H
H
Nakamura
H
H
Morooka
O
O
Shibata
K
K
Sumikawa
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid; D002850:Chromatography, Gel; D004789:Enzyme Activation; D006851:Hydrochloric Acid / Q000494:pharmacology / Q000633:toxicity*; D001239:Inhalation; D008297:Male; D010741:Phospholipases A / Q000302:isolation & purification / Q000378:metabolism*; D054467:Phospholipases A2; D011650:Pulmonary Alveoli / Q000187:drug effects* / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar
D010741:Phospholipases A; D054467:Phospholipases A2; D006851:Hydrochloric Acid