Sarcocystis neurona and Sarcocystis falcatula: monitoring of schizogony in cell culture using fluorescent nuclear labeling.
The nuclei of merozoites of Sarcocystis neurona and Sarcocystis falcatula were labeled with the fluorescent marker Syto21. It was shown that the marker would label the parasites and that they would retain the marker throughout schizogony. Thus, there was sufficient marker in the daughter merozoites to make them easily visible with fluorescence microscopy. This technique will be helpful in studying the developmental biology of these parasites in vitro.
Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA. ddb3@cornell.edu
D D
DD
Bowman
M A
MA
Iordanescu
H H
HH
Chou
K H
KH
Horton
D000818:Animals; D002417:Cattle; D005456:Fluorescent Dyes; D008856:Microscopy, Fluorescence / Q000662:veterinary*; D009930:Organic Chemicals; D012522:Sarcocystis / Q000648:ultrastructure*; D013045:Species Specificity
D005456:Fluorescent Dyes; D009930:Organic Chemicals; C428827:SYTO 21