Cloning and sequencing of cynomolgus macaque CCR3, GPR15, and STRL33: potential coreceptors for HIV type 1, HIV type 2, and SIV.
The characterization of several seven-transmembrane G protein-coupled receptors, which function as coreceptors for HIV-1, HIV-2, and/or SIV, has opened up a whole new area of AIDS research. Animal models that have played a central role in the understanding of lentivirus pathogenesis and the design of novel vaccine strategies may also be invaluable in studying the role of these secondary receptors in infection and disease progression. However, since it is known that minor species-specific sequence changes in CCR3 and STRL33 affect their ability to act as coreceptors for HIV-1, HIV-2, and/or SIV, it is important to ascertain whether the relevant receptors function as expected in the animal model of choice. Many studies have been performed on the function of rhesus macaque receptors, but not on the cynomolgus macaque equivalents. Both species are used as animal models for lentivirus pathogenesis, but since there are differences in their susceptibility to viral infection, we felt it was important for information to be available for both rhesus and cynomolgus macaque receptors. The sequence of three cynomolgus macaque receptors, CCR3, GPR15, and STRL33, are presented in this sequence note. These sequences are compared with already published human and rhesus macaque homologs. Functional studies are currently being performed on these three cynomolgus macaque receptors to determine their ability to function as coreceptors for HIV-2, SIV, and/or SHIV isolates.
National Institute for Biological Standards and Control, South Mimms, Potters Bar, Herts, EN6 3QG UK. awadeevans@nibsc.ac.uk
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Wade-Evans
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Russell
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Jenkins
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Javan
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D015658:HIV Infections / Q000821:virology; D015497:HIV-1 / Q000378:metabolism; D015498:HIV-2 / Q000378:metabolism; D006801:Humans; D008252:Macaca fascicularis; D008969:Molecular Sequence Data; D054397:Receptors, CCR3; D000075744:Receptors, CXCR6; D019707:Receptors, Chemokine / Q000235:genetics* / Q000378:metabolism; D018121:Receptors, Cytokine / Q000235:genetics* / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled; D015728:Receptors, HIV / Q000378:metabolism; D018000:Receptors, Peptide / Q000235:genetics* / Q000378:metabolism; D011991:Receptors, Virus; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D016097:Simian Acquired Immunodeficiency Syndrome / Q000821:virology; D015302:Simian Immunodeficiency Virus / Q000378:metabolism
C515350:CCR3 protein, human; C106330:CXCR6 protein, human; C103539:GPR15 protein, human; D054397:Receptors, CCR3; D000075744:Receptors, CXCR6; D019707:Receptors, Chemokine; D018121:Receptors, Cytokine; D043562:Receptors, G-Protein-Coupled; D015728:Receptors, HIV; D018000:Receptors, Peptide; D011991:Receptors, Virus