A unipolarly located, cell-surface-associated agglutinin, RapA, belongs to a family of Rhizobium-adhering proteins (Rap) in Rhizobium leguminosarum bv. trifolii.
The phage-display cloning technique was used to find rhizobial proteins that bind to receptors located on the bacterial cell surface. The aim was to clone the gene(s) encoding rhicadhesin, a universal rhizobial adhesion protein, and/or other cell-surface-binding proteins. Four such Rhizobium-adhering proteins (Rap) were revealed in Rhizobium leguminosarum bv. trifolii strain R200. The binding is mediated by homologous Ra domains in these proteins. One member of the Rap protein family, named RapA1, is a secreted calcium-binding protein, which are also properties expected for rhicadhesin. However, the size of the protein (24 kDa instead of 14 kDa) and its distribution among different rhizobia (present in only Rhizobium leguminosarum biovars and R. etli instead of all members of Rhizobiaceae argue against RapA1 being rhicadhesin. Protein RapA1 consists of two homologous Ra domains and agglutinates R200 cells by binding to specific receptors located at one cell pole during exponential growth. Expression of these cell-surface receptors was detected only in rhizobia that produce the RapA proteins. The authors propose that the homologous Ra domains, found to be present also in other proteins with different structure, represent lectin domains, which confer upon these proteins the ability to recognize their cognate carbohydrate structures.
Ausmees
Nora
N
Swedish University of Agricultural Sciences, SLU, Department of Microbiology, Box 7025, S-75007 Uppsala, Sweden1.
Jacobsson
Karin
K
Swedish University of Agricultural Sciences, SLU, Department of Microbiology, Box 7025, S-75007 Uppsala, Sweden1.
Lindberg
Martin
M
Swedish University of Agricultural Sciences, SLU, Department of Microbiology, Box 7025, S-75007 Uppsala, Sweden1.
D018829:Adhesins, Bacterial / Q000235:genetics* / Q000378:metabolism*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005798:Genes, Bacterial; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D005810:Multigene Family; D019151:Peptide Library; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012231:Rhizobium / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D002135:Calcium-Binding Proteins; D019151:Peptide Library; D011956:Receptors, Cell Surface; C053477:adhesin, Rhizobium