[The change of vWF in vascular endothelial cells under different stress].
OBJECTIVE
To correlate the injury of vascular endothelial cells during various pathological conditions with the change of vWF (von Willebrand Factor) in different VEC lines.


METHODS
Flow cytometer(FCM) were used to defect the immunoflourescent stained vWF in pulmonary artery endothelial cells (PAEC) of pig and aortic endothelial cells(AEC) of rats.


RESULTS
The positive rates of vWF in PAEC of pigs is similar with that in AEC of rats under normal condition, but it decreased differently after hypoxic or cold injury. It was very interesting that the mean fluorescence intensity of positive PAEC or AEC exposed to hypoxia or cold elevated significantly compared with those of control.


CONCLUSIONS
The change of vWF in VEC can be used to evaluate the function of VEC under different stress.
Institute of Health and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050.
X
X
Hong
F Z
FZ
Li
Z Y
ZY
Yin
P H
PH
Yan
Y X
YX
He
D000818:Animals; D015687:Cell Hypoxia; D003080:Cold Temperature / Q000009:adverse effects; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000473:pathology; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D010446:Peptide Fragments / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013312:Stress, Physiological / Q000378:metabolism; D013552:Swine; D014841:von Willebrand Factor / Q000378:metabolism*
D010446:Peptide Fragments; D014841:von Willebrand Factor; C090953:von Willebrand factor (504-728)