[Studies of multidrug resistance gene expression and apoptosis in stomach carcinoma].
OBJECTIVE
To investigate the influence of multidrug resistance gene (MDR) expression and apoptosis on stomach cancer.


METHODS
A total of 80 cases of paraffin embedded stomach cancer tissues was stained immunohistochemically for P-gp, GST-pi and TOPO II using monoclonal antibodies and apoptosis (in terms of apoptotic index, AI) in tissues was musured by the TUNEL method. The results were correlated with tumor type, clinical stage and prognosis.


RESULTS
Normal stomach tissue expression of P-gp was associated with better survival, but higher level of P-gp expression in tumor tissues was associated with poor prognosis. The expression of GST-pi didnot show a relationship with cell differentiation of stomach cancer and clinical stage of the disease, but positive correlation did exist between intensity of expression and survival time. Although the expression of TOPO II was associated with tumor cell differentiation, it did not affect prognosis. Apoptosis was demonstrated in 73% of the cases and was related to clinical stage of stomach carcinoma.


CONCLUSIONS
While P-gp expression in tumor cells is indicative of poor prognosis, its expression in normal tissue has the opposite effect. Positive correlation exits between GST-pi expression intensity in tumor cells and survival. MDR and apoptosis are related to the biologic behavior of the tumor.
Department of Oncology, Fujian Provincial Hospital, Fuzhou 350001, China.
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D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000096:biosynthesis; D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000235:genetics*; D004250:DNA Topoisomerases, Type II / Q000096:biosynthesis; D005260:Female; D019450:Genes, MDR / Q000235:genetics*; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase / Q000096:biosynthesis; D006801:Humans; D020287:In Situ Nick-End Labeling; D007527:Isoenzymes / Q000096:biosynthesis; D008297:Male; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D007527:Isoenzymes; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; D004250:DNA Topoisomerases, Type II