[Expression of CD44V6 and its effects on cytoskeleton in human colorectal carcinoma cell lines].
OBJECTIVE
To observe the expression of CD44V6 and its effects on cytoskeleton in human colorectal carcinoma(HCC) HT29 and LoVo cell lines.


METHODS
Expression of CD44V6 variant in HCC HT29 and LoVo cell lines was investigated by in situ hybridization, immunohistochemistry and quantitative immunofluorescence technique. The effects of CD44V6 variants on cytoskeleton was studied by immunofluorescence and confocal 3 dimension reconstruction technique.


RESULTS
CD44V6 was expressed at the mRNA and protein levels in both HT29 and LoVo cell lines, but the expression was higher in LoVo cells than in HT29 cells. When the HT29 cells and LoVo cells were blocked by monoclonal antibodies, it was found that changes developed in the cytoskeleton of these cells.


CONCLUSIONS
The expression of CD44V6 is related to the metastatic potentiality of human colorectal carcinoma cells. CD44V6 may affect the distribution, polymerization and depolymerization of actin of HCC cells and promote HCC cell metastasis.
Department of Pathology, First Military Medical University, Guangzhou 510515.
Z
Z
Li
Y
Y
Ding
Y
Y
Deng
D000199:Actins / Q000378:metabolism; D000230:Adenocarcinoma / Q000378:metabolism*; D003110:Colonic Neoplasms / Q000378:metabolism*; D003599:Cytoskeleton / Q000378:metabolism* / Q000473:pathology; D006023:Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D018960:Hyaluronan Receptors / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014407:Tumor Cells, Cultured
D000199:Actins; C112697:CD44v6 antigen; D006023:Glycoproteins; D018960:Hyaluronan Receptors; D012333:RNA, Messenger