Nanoscale fluorescent sensors for intracellular analysis.
There is a growing interest in the development of submicron optochemical sensing devices. Miniaturization of sensors to nano-dimensions decreases their typical response time down to the millisecond time scale. Their penetration volume is reduced to a few cubic micrometers and they exhibit a spatial resolution at the nanometer scale. In this review the fabrication of submicron optical fiber fluorescent sensors and particle-based fluorescent nanosensors is described. The functional characteristics of these exciting miniaturized fluorescent sensors and their applications for quantitative measurement of intracellular analytes are demonstrated.
Lu
J
J
University of New Orleans, Department of Chemistry, LA 70148, USA.
Rosenzweig
Z
Z
D000818:Animals; D015374:Biosensing Techniques; D005336:Fiber Optic Technology; D005456:Fluorescent Dyes / Q000737:chemistry*; D008264:Macrophages / Q000378:metabolism; D008904:Miniaturization; D055100:Optical Fibers; D010100:Oxygen / Q000032:analysis; D013194:Staining and Labeling / Q000379:methods*
D005456:Fluorescent Dyes; D010100:Oxygen