Fluorimetric determination of methylmercury as an ion-association complex with rhodamine B in the presence of iodide.
A fluorimetric method for the determination of methylmercury was established. The method was based on the formation of an ionic pair between iodide-methylmercury-rhodamine B in hydrochloric acid, which can be extracted with benzene. The fluorescence emission was measured at lambda(ex)/lambda(em) 575/590 nm, and the experimental variables and possible interference were studied. The linear calibration range was 4 x 10(-8) mol/L to approximately 5 x 10(-7) mol/L with a correlation coefficient of 0.9992. The detection limit was 1 x 10(-8) mol/L. The method was used to determine methylmercury in human hair. The recovery was in the range of 91% to 105% and the relative standard deviation was 2.8%. The results agreed with those obtained by gas chromatography with electron capture detection.
Li
H B
HB
Department of Botany, The University of Hong Kong, Hong Kong.
Chen
F
F
Xu
X R
XR
D002138:Calibration; D005470:Fluorometry / Q000379:methods / Q000592:standards; D006197:Hair / Q000737:chemistry; D006801:Humans; D008767:Methylmercury Compounds / Q000032:analysis* / Q000737:chemistry / Q000592:standards; D011193:Potassium Iodide / Q000737:chemistry / Q000494:pharmacology; D012015:Reference Standards; D012235:Rhodamines / Q000737:chemistry / Q000494:pharmacology
D008767:Methylmercury Compounds; D012235:Rhodamines; D011193:Potassium Iodide; C029773:rhodamine B; C004925:methylmercuric chloride