[Bcl-2 anti-sense oligonucleotide sensitizes Fas-mediated apoptosis of gastric cancer cells].
OBJECTIVE
To study the effect of bcl-2 anti-sense oligonucleotide on the sensitivity of gastric cancer cells to Fas-mediated apoptosis.


METHODS
Gastric cancer cell line MKN45 was transfected with bcl-2 anti-sense oligonucleotide, and expression of bcl-2 was examined by Western blotting. Agonistic Fas antibody was used to induce apoptosis detected by TUNEL staining and flow cytometry.


RESULTS
Bcl-2 expression in MKN45 cells transfected with bcl-2 anti-sense oligonucleotide was markedly inhibited. When cultured with antibody apoptosis index of the anti-sense oligonucleotide-treated MKN45 cells was 55.6% +/- 4.7% (n = 5), which was significantly higher than that of the control (8.4% +/- 2.1%, n = 5).


CONCLUSIONS
Expression of bcl-2 in gastric cancer cells may antagonize Fas-mediated apoptosis.
Department of Surgery, People's Hospital of Taishan, Taishan 529200, China.
J
J
Cao
Y
Y
Qiao
J
J
Min
D017209:Apoptosis / Q000187:drug effects*; D019254:Genes, bcl-2 / Q000235:genetics*; D006801:Humans; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology*; D014162:Transfection; D014407:Tumor Cells, Cultured; D019014:fas Receptor / Q000276:immunology
D020319:Oligodeoxyribonucleotides, Antisense; D019014:fas Receptor