Biocompatibility and osseointegration in osteoporotic bone.
We implanted nails made of titanium (Ti6Al4V) and of two types of glass ceramic material (RKKP and AP40) into healthy and osteopenic rats. After two months, a histomorphometric analysis was performed and the affinity index calculated. In addition, osteoblasts from normal and osteopenic bone were cultured and the biomaterials were evaluated in vitro. In normal bone the rate of osseointegration was similar for all materials tested (p < 0.5) while in osteopenic bone AP40 did not osseointegrate (p < 0.0005). In vitro, no differences were observed for all biomaterials when cultured in normal bone-derived cells whereas in osteopenic-bone-derived cells there was a significant difference in some of the tested parameters when using AP40. Our findings suggest that osteopenic models may be used in vivo in the preclinical evaluation of orthopaedic biomaterials. We suggest that primary cell cultures from pathological models could be used as an experimental model in vitro.
Experimental Surgery Department, Rizzoli Orthopaedic Institute, Bologna, Italy.
M
M
Fini
G
G
Giavaresi
P
P
Torricelli
A
A
Krajewski
A
A
Ravaglioli
M M
MM
Belmonte
G
G
Biagini
R
R
Giardino
D000497:Alloys; D000818:Animals; D001858:Bone Nails; D002516:Ceramics; D005260:Female; D005269:Femur / Q000473:pathology; D008422:Materials Testing; D016348:Osseointegration / Q000502:physiology*; D010024:Osteoporosis / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014025:Titanium
D000497:Alloys; C031462:titanium alloy (TiAl6V4); C031323:bio-vitroceramic AP40; D014025:Titanium