Matrix metalloproteinases expression in HL-60 promyelocytic leukemia cells during apoptosis.
Human promyelocytic leukemia HL-60 cells have been used as a model to study both the expression of matrix-metalloproteinases and the mechanisms of programmed cell death. In the present study we examined the expression of these proteases in HL-60 cells stimulated by different apoptotic triggers. As shown by zymography, HL-60 cells released three major isofroms of the matrix-degrading proteases; when the leukemic cells were grown in serum-free conditions, as well as after hyperthermia and methotrexate treatment, we found a significant loss of the constitutive production of the 92 kDa matrixmetalloprotease, with an unequivocable molecular and ultrastructural evidence of programmed cell death. These results suggest that in HL-60 cells the expression/release of matrix metalloproteases can be down-regulated in the presence of the apoptotic-induced alterations, and that the decreased matrix-degrading capacity of this leukemic cell line during apoptosis may reduce its invasive potential.
Gazzanelli
G
G
Istituto di Istologia ed Analisi di Laboratorio, Facoltà di Scienze MFN, Università degli Studi, Via E. Zeppi, 61029 Urbino, Italy.
Luchetti
F
F
Burattini
S
S
Mannello
F
F
Falcieri
E
E
Papa
S
S
D017209:Apoptosis / Q000502:physiology*; D048429:Cell Size; D016895:Culture Media, Serum-Free; D053938:DNA Fragmentation; D018922:HL-60 Cells; D006801:Humans; D020782:Matrix Metalloproteinases / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors
D016895:Culture Media, Serum-Free; D020782:Matrix Metalloproteinases