[Msp I and Rsa I RFLPs of vWF gene in Chinese Han people].
OBJECTIVE
To study the Msp I and Rsa I restriction fragment length polymorphisms (RFLPs) in the 5' region of human von Willebrand factor gene in Chinese Han people.


METHODS
A 226 bp amplified fragment of human vWF gene from 52 unrelated individuals and a 322 bp amplified fragment from 48 individuals were analyzed by Msp I or Rsa I digestion.


RESULTS
Complete linkage disequilibrium was observed at both Msp I and Rsa I sites in vWF gene in Chinese Han people. The frequencies at the two sites were 0.20/0.80 for Msp I+/Msp I- and 0.93/0.07 for Rsa I+/Rsa I-. The theoretical heterozygous rates were 36% and 10%, respectively.


CONCLUSIONS
The Msp I and Rsa I RELPs of vWF gene are useful for genetic counseling and prenatal diagnosis of vWD in Chinese Han people.
Thrombosis and Haemostasis Research Unit, Jiangsu Institute of Hematology, First Affiliated Hospital, Suzhou Medical College, Suzhou 215004.
Z
Z
Li
H
H
Wan
H
H
Tai
D000483:Alleles; D044466:Asian People; D005006:Ethnicity; D006801:Humans; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D014842:von Willebrand Diseases / Q000235:genetics*; D014841:von Willebrand Factor / Q000235:genetics*
D014841:von Willebrand Factor