[Significance and features of MLC reaction in histocompatibility determination of donor and recipient in allogenic bone marrow transplantation to patients with hematological malignancies].
OBJECTIVE
To characterize mixed lymphocyte culture (MLC) reaction used for determination of donor-recipient compatibility before bone marrow transplantation to patients with hematological malignancies and to assess the reaction significance.


METHODS
The analysis was made of compatibility testing in MLC standard reaction performed in 134 patients with hematological malignancies with HLA-A, -B identical donors-sibs and in 5 patients with haploid identical donors.


RESULTS
Out of 134 patients, 22(91%) appeared compatible to donor sibs in MLC reaction, 12(9%) patients were incompatible. Mean RR for MLC-compatible couples made up: in RvD direction 77 +/- 0.17%, DvR 2.61 +/- 0.32%. 93% of RR values ranged from +15 to -15%, the rest--from +25% to -25%. Bone marrow transplantation was made in 83 patients. Graft retention was observed in 77(93%) patients. Acute and chronic graft versus host reaction developed in 15 and 17 patients, respectively.


CONCLUSIONS
An optimal protocol is proposed for examination of compatibility donor-recipient in MLC reaction in patients with hematological malignancies. It is intended for allogenic bone marrow transplantation in hematological departments.
A P
AP
Shpakova
T I
TI
Bulycheva
L S
LS
Liubimova
L P
LP
Mendeleeva
D000293:Adolescent; D000328:Adult; D016026:Bone Marrow Transplantation / Q000276:immunology; D002648:Child; D005260:Female; D006086:Graft vs Host Disease / Q000276:immunology / Q000517:prevention & control; D015234:HLA-A Antigens / Q000276:immunology; D015235:HLA-B Antigens / Q000276:immunology; D019337:Hematologic Neoplasms / Q000276:immunology / Q000628:therapy*; D006648:Histocompatibility / Q000276:immunology*; D006801:Humans; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D008875:Middle Aged; D016747:Minor Lymphocyte Stimulatory Antigens / Q000276:immunology*; D011379:Prognosis; D012189:Retrospective Studies; D014019:Tissue Donors; D014184:Transplantation, Homologous
D015234:HLA-A Antigens; D015235:HLA-B Antigens; D016747:Minor Lymphocyte Stimulatory Antigens