Protective responses against experimental Mycobacterium leprae infection in mice induced by recombinant Bacillus Calmette-Guérin over-producing three putative protective antigen candidates.
The components of Ag85 (Ag85A, Ag85B, and Ag85C) are putative protective antigen candidates against mycobacterial infection. A recombinant Mycobacterium bovis Bacillus Calmette-Guérin (rBCG) over-producing Ag85A, Ag85B, and MPB51 (rBCG/BA51) was constructed. rBCG/BA51 could secrete these antigens at levels more than five times higher than parental BCG. Immunization of C57BL/6 and BALB/c mice with this rBCG reduced the multiplication of Mycobacterium leprae in the foot pads of both strains of mice. The inhibition by rBCG/BA51 was more evident than that by parental BCG.
Nagasaki University School of Dentistry, Sakamoto 1-7-1, 852-8588, Nagasaki, Japan. oharan@net.nagasaki-u.ac.jp
N
N
Ohara
M
M
Matsuoka
H
H
Nomaguchi
M
M
Naito
T
T
Yamada
D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics; D001500:BCG Vaccine / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D007918:Leprosy / Q000276:immunology / Q000517:prevention & control; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009163:Mycobacterium bovis / Q000235:genetics / Q000276:immunology; D009166:Mycobacterium leprae / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D010957:Plasmids / Q000235:genetics; D013154:Spleen / Q000276:immunology; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology / Q000494:pharmacology
D000942:Antigens, Bacterial; D001500:BCG Vaccine; D017931:DNA Primers; D014614:Vaccines, Synthetic