[Dose-dependent effects of the combined beta/gamma emitter 186-Rhenium on the growth of human vessel wall cells].
OBJECTIVE
The aim of this study was to evaluate the capability of human aortic smooth musc e cells (HaSMC) and endothelial cells (EC) to recover after incubation with the combined beta/gamma emitter 186rhenium.


METHODS
Two days after plating, HaSMC and EC were incubated for five days with 186Re (total doses applied 4 Gy-32 Gy). Cell counts were performed for a period of 30 days (haSMC) and 22 days (EC). To detect possible growth recovery, colony formation assays were plated for both cell types on day 5, 10, and 20 (and lay 30 for haSMC).


RESULTS
Both cell types presented a dose-dependent growth inhibition which was maximum at a dose of 32 Gy. Human endothelial cells presented with total growth recovery at 4 and 8 Gy, and a partial growth recovery at 16 Gy. Smooth muscle cells only presented partial growth recovery at 4 and 8 Gy. At 16 Gy and more no recovery was detected.


CONCLUSIONS
HaSMC as well as EC growth can be modulated effectively with 186Re over a period of 30 days in vitro. Compared to smooth muscle cells human endothelial cellls seem to possess a higher potential to recover at doses of 8 to 16 Gy. 186Re may be a valuable radionuclide to prevent restenosis.
Wiskirchen
J
J
Abteilung für Radiologische Diagnostik, Eberhard-Karls-Universität, Tübingen. jakub.wiskirchen@uni-tuebingen.de
Dittmann
H
H
Gebert
R
R
Burgbacher
B
B
Betsch
A
A
Schöber
W
W
Johst
U
U
Dohmen
B M
BM
Kehlbach
R
R
Rodemann
H P
HP
Bares
R
R
Duda
S H
SH
D000800:Angioplasty, Balloon; D015906:Angioplasty, Balloon, Coronary; D001011:Aorta / Q000166:cytology; D002452:Cell Count; D002455:Cell Division; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology* / Q000528:radiation effects*; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000528:radiation effects*; D011829:Radiation Dosage; D011868:Radioisotopes / Q000494:pharmacology*; D012008:Recurrence; D012211:Rhenium / Q000494:pharmacology*; D013997:Time Factors
D011868:Radioisotopes; D012211:Rhenium