[Effect of enterogastric circulation of diazepam on its CNS inhibitory action in mice].
The present study was designed to examine whether disturbance of the enterogastric circulation of diazepam would obviously affect its inhibitory action on CNS. Intragastric administration of acidic liquid (pH 1) elicited a marked increase in the amount of diazepam in the gastric juice of mice 1 h after i.v. diazepam, as measured by HPLC method. The effects of ig acidic charcoal (2 g.kg-1, pH 1), neutral charcoal and normal saline on diazepam-induced CNS depression were compared by studying the duration of hypnotic action, inhibition of spontaneous activity, dropping rate in rotating rod test and mortality after i.v. diazepam (10-40 mg.kg-1) in mice. The results showed that the animals receiving ig acidic charcoal recovered more rapidly from CNS depression and exhibited lower mortality than the animals in neutral charcoal group and normal saline group.
Department of Pharmacology, Wannan Medical College, Wuhu 241001.
Y M
YM
Ma
N N
NN
Cheng
S P
SP
Li
R Y
RY
Sun
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D002606:Charcoal; D003864:Depression, Chemical; D003975:Diazepam / Q000493:pharmacokinetics / Q000494:pharmacology*; D005260:Female; D005744:Gastric Acid / Q000378:metabolism*; D007275:Injections, Intravenous; D008297:Male; D051379:Mice; D012890:Sleep / Q000187:drug effects*
D002606:Charcoal; D003975:Diazepam