Construction of gene-targeting vectors: a rapid Mu in vitro DNA transposition-based strategy generating null, potentially hypomorphic, and conditional alleles.
Gene targeting into mammalian genomes by means of homologous recombination is a powerful technique for analyzing gene function through generation of transgenic animals. Hundreds of mouse strains carrying targeted alleles have already been created and recent modifications of the technology, in particular generation of conditional alleles, have extended the usefulness of the methodology for a variety of special purposes. Even though the standard protocols, including the construction of gene-targeting vector plasmids, are relatively straightforward, they typically involve time-consuming and laborious gene mapping and/or sequencing steps. To produce various types of gene-targeting constructions rapidly and with minimum effort, we developed a strategy, that utilizes a highly efficient in vitro transposition reaction of phage Mu, and tested it in a targeting of the mouse Kcc2 gene locus. A vast number and different types of targeting constructions can be generated simultaneously with little or no prior sequence knowledge of the gene locus of interest. This quick and efficient general strategy will facilitate easy generation of null, potentially hypomorphic, and conditional alleles. Especially useful it will be in the cases when effects of several exons within a given gene are to be studied, a task that necessarily will involve generation of multiple constructions. The strategy extends the use of diverse recombination reactions for advanced genome engineering and complements existing recombination-based approaches for generation of gene-targeting constructions.
Vilen
H
H
Institute of Biotechnology, Viikki Biocenter, University of Helsinki, Finland.
Eerikäinen
S
S
Tornberg
J
J
Airaksinen
M S
MS
Savilahti
H
H
D000483:Alleles; D000818:Animals; D010583:Bacteriophage mu / Q000235:genetics*; D015153:Blotting, Western; D002352:Carrier Proteins / Q000235:genetics* / Q000502:physiology; D002478:Cells, Cultured; D004254:DNA Nucleotidyltransferases / Q000235:genetics; D017931:DNA Primers / Q000737:chemistry; D004251:DNA Transposable Elements / Q000235:genetics*; D018274:Electroporation; D005786:Gene Expression Regulation; D018390:Gene Targeting; D005822:Genetic Vectors; D066298:In Vitro Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D015183:Restriction Mapping; D027981:Symporters; D000096922:K Cl- Cotransporters
D002352:Carrier Proteins; D004254:DNA Nucleotidyltransferases; D017931:DNA Primers; D004251:DNA Transposable Elements; D027981:Symporters; D000096922:K Cl- Cotransporters