Behavior and activities in the central nervous system after activation of L-type calcium channels in mice.
The distribution of the immediate early gene c-fos expression in the mouse central nervous system after subcutaneous injection of Bay K 8644 was observed immunohistochemically. Half an hour after injection, c-fos protein (FOS) was expressed in the piriform cortex, sensorimotor cortex, caudate putamen, thalamic paraventricular nucleus and striate cortex, etc. Intense FOS immunoreactive (FOS-ir) cells were seen during 2-4 h after injection. The results suggested that the distribution of FOS-ir cells after subcutaneous injection of Bay K 8644 was coincident with that of L-type calcium channels in the different areas of the CNS. After Bay K 8644 injection, mice appeared seizure-like behavior. The percentage of cells double-labelled by FOS and CaBP immunoreactivities in the observed regions was about 60.2-72.8% in CaBP-ir cells. It suggested that most CaBP-ir cells may have L-type calcium channels.
Beijing Institute for Neuroscience, Capital University of Medical Sciences, Beijing 100054.
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D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002120:Calcium Channel Agonists / Q000494:pharmacology; D020746:Calcium Channels, L-Type / Q000502:physiology*; D005260:Female; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis*; D012640:Seizures / Q000139:chemically induced*
D002120:Calcium Channel Agonists; D020746:Calcium Channels, L-Type; D016760:Proto-Oncogene Proteins c-fos; D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester