In vitro reproductive toxicity of polychlorinated biphenyls: effects on oocyte maturation and developmental competence in cattle.
Polychlorinated biphenyls (PCBs) are one of the most persistent and widespread group of endocrine disrupting compounds in the ecosystem. High concentrations of these substances are known to be present in sewage sludge from industrial, agricultural, and domestic origin that is spread in increasing amounts on arable land and pasture as fertilizer and is found in water, representing an increasing risk for the reproductive health of farm animals. Objective of this study was to determine the impact of PCBs on maturation and developmental competence of cattle oocytes. Since PCBs are a family of 209 molecules present in the environment as a mixture, Aroclor-1254, a pool of more than 60 congeners, was used in these experiments as its composition is considered to be environmentally relevant. Cumulus-oocytes complexes were exposed during IVM to serial concentrations of Aroclor-1254 (between 1 microg/ml and 0.0001 microg/ml) and compared with control groups. Aroclor decreased the percentage of oocytes that reached metaphase II stage after 24 hr, at doses as low as 0.01 microg/ml. Groups treated with 0.001 microg/ml or above, showed an impaired fertilization rate and a dramatic increase of polyspermy. Moreover, exposure during maturation resulted in a reduced proportion of oocytes that cleaved and developed until blastocyst stage although no differences in embryo cell numbers were observed. The present study indicates that very low PCBs concentrations are sufficient to disrupt bovine oocyte maturation, its fertilization, and developmental competence. These results also provide a set of reference data for the assessment of the risk posed by these substances to animal reproductive health, though further work will be necessary to equate in vitro doses to in vivo exposures.
Pocar
P
P
Department of Anatomy of Domestic Animals, University of Milan, Italy.
Perazzoli
F
F
Luciano
A M
AM
Gandolfi
F
F
D000818:Animals; D001755:Blastocyst / Q000187:drug effects; D002417:Cattle; D002418:Cattle Diseases / Q000139:chemically induced*; D020111:Chlorodiphenyl (54% Chlorine) / Q000009:adverse effects / Q000494:pharmacology / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D005314:Embryonic and Fetal Development / Q000187:drug effects; D004781:Environmental Exposure; D005260:Female; D005307:Fertilization in Vitro; D007220:Industrial Waste; D007247:Infertility, Female / Q000139:chemically induced / Q000662:veterinary*; D008297:Male; D008540:Meiosis / Q000187:drug effects; D009865:Oocytes / Q000187:drug effects*; D009866:Oogenesis / Q000187:drug effects*; D011078:Polychlorinated Biphenyls / Q000009:adverse effects* / Q000494:pharmacology; D012722:Sewage; D012989:Soil Pollutants / Q000009:adverse effects* / Q000494:pharmacology; D014874:Water Pollutants, Chemical / Q000009:adverse effects* / Q000494:pharmacology; D015053:Zygote / Q000187:drug effects
D007220:Industrial Waste; D012722:Sewage; D012989:Soil Pollutants; D014874:Water Pollutants, Chemical; D020111:Chlorodiphenyl (54% Chlorine); D011078:Polychlorinated Biphenyls