Brown Norway chromosome 13 confers protection from high salt to consomic Dahl S rat.
Consomic rats (SS.BN13), in which chromosome 13 from normotensive inbred Brown Norway rats from a colony maintained at the Medical College of Wisconsin (BN/Mcw) was introgressed into the background of Dahl salt-sensitive (SS/Mcw) rats, also maintained in a colony at the Medical College of Wisconsin, were bred. The present studies determined the mean arterial pressure (MAP) responses to salt and renal and peripheral vascular responses to norepinephrine and angiotensin II; 24-hour protein excretion and histological analyses were used to assess renal pathology in rats that received a high salt (4% NaCl) diet for 4 weeks. MAP of rats measured daily during the fourth week averaged 170+/-3.3 mm Hg in SS/Mcw rats, 119+/-2.1 mm Hg in SS.BN13 rats, and 103+/-1.3 mm Hg in BN/Mcw rats. After salt depletion, MAP fell an average of 27+/-4.5 mm Hg in SS/Mcw rats, 9+/-2.6 mm Hg in SS.BN13 rats, and 11+/-3.0 mm Hg in BN/Mcw rats. Protein excretion of SS/Mcw rats on a high salt diet averaged 189+/-30 mg/24 h, 63+/-18 mg/24 h in SS.BN13 rats, and 40+/-6.4 mg/24 h in BN/Mcw rats. Compared with SS.BN13 and BN/Mcw rats, SS/Mcw rats exhibited significantly greater increases of renal vascular resistance in response to intravenous norepinephrine and angiotensin II. Severe medullary interstitial fibrosis and tubular necrosis after a high salt diet were found consistently in SS/Mcw rat kidneys but were largely absent in the SS.BN13 and BN/Mcw rat kidneys. A similar degree of glomerular sclerosis was found in both SS/Mcw and SS.BN13 rats. In rats fed a 0.4% salt diet, the glomerular filtration rate of SS/Mcw rats was significantly less than that of BN/Mcw and SS.BN13 rats. These results reveal a powerful gene, or set of genes, within chromosome 13 of BN/Mcw rats that confers protection from the detrimental effects of high salt to the SS/Mcw rats.
Department of Physiology, Medical College of Wisconsin, Milwaukee, USA. cowley@mcw.edu
A W
AW
Cowley
R J
RJ
Roman
M L
ML
Kaldunski
P
P
Dumas
J G
JG
Dickhout
A S
AS
Greene
H J
HJ
Jacob
D000804:Angiotensin II; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D005355:Fibrosis; D005665:Furosemide; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005838:Genotype; D006973:Hypertension / Q000235:genetics / Q000628:therapy* / Q000652:urine; D007678:Kidney Glomerulus / Q000187:drug effects / Q000473:pathology; D007679:Kidney Medulla / Q000187:drug effects / Q000473:pathology; D007684:Kidney Tubules / Q000187:drug effects / Q000473:pathology; D009336:Necrosis; D009638:Norepinephrine; D011507:Proteinuria / Q000652:urine; D051381:Rats; D011914:Rats, Inbred BN / Q000235:genetics*; D020303:Rats, Inbred Dahl / Q000235:genetics*; D012077:Renal Artery / Q000187:drug effects; D012083:Renin / Q000235:genetics*; D012598:Sclerosis; D012965:Sodium Chloride / Q000008:administration & dosage / Q000633:toxicity; D012982:Sodium, Dietary / Q000008:administration & dosage / Q000633:toxicity*; D014655:Vascular Resistance / Q000187:drug effects
D012982:Sodium, Dietary; D000804:Angiotensin II; D012965:Sodium Chloride; D005665:Furosemide; D012083:Renin; D009638:Norepinephrine