Detection of fungi in the nasal mucosa using polymerase chain reaction.
OBJECTIVE
Fungi have been increasingly recognized as important pathogens in sinusitis. However, detection of fungus with conventional culture techniques is insensitive and unreliable. Polymerase chain reaction (PCR) is an exquisitely sensitive assay that can detect the DNA of 10 or less fungal elements. The aim of this study was to compare the sensitivity of conventional culture techniques using PCR analysis.


METHODS
Nasal swabs and DNA samples were collected from the nasal cavities of control subjects and patients with chronic sinusitis. Fungal-specific PCR analysis and standard cultures were performed on every sample. chi2 analysis was used to test for statistical differences between groups.


RESULTS
PCR analysis detected fungal DNA in 42% and 40% of control subjects and patients with chronic sinusitis while standard cultures were positive in 7% and 0%, respectively. There was no statistically significant difference in the prevalence of fungi in the normal volunteers and patients with chronic rhinosinusitis.


CONCLUSIONS
PCR is significantly more sensitive than nasal swab cultures in detecting the presence of fungi in nasal mucosa. In addition, our study suggests that the presence of fungi alone is insufficient to implicate it as the pathogen in chronic sinusitis.
Laboratory of Molecular Otology, University of California-San Francisco, San Francisco, CA 94143-0342, USA.
M D
MD
Catten
A H
AH
Murr
J A
JA
Goldstein
A N
AN
Mhatre
A K
AK
Lalwani
D002908:Chronic Disease; D004271:DNA, Fungal / Q000032:analysis*; D005658:Fungi / Q000302:isolation & purification*; D006801:Humans; D009297:Nasal Mucosa / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012852:Sinusitis / Q000382:microbiology
D004271:DNA, Fungal