cDNA cloning and molecular analysis of papaya small GTP-binding protein, pgp1.
In the course of papaya EST collection, one clone (pRA4-3) encoding partial sequence of papaya small GTP-binding protein gene, pgp1, was obtained. Based on the sequence information of pRA4-3, the entire coding region of pgp1 was cloned using the 3'RACE PCR technique. ORF of pgp1 is 636bp long and deduced molecular weight of the protein is 23,311. Phylogenetic analysis showed that PGP1 belongs to YPT/RAB group of the small GTP-binding protein and is a homologue of RAB2. Southern analysis showed that there are several pgp1-related genes in papaya genome. Northern analysis showed that pgp1 was expressed equally in stems of seedlings that were grown under light and dark conditions. This result shows that PGP1 is not involved in the phytochrome-mediated signal transduction as an auxin signal transducer in stems of papaya seedlings.
Iwate Biotechnology Research Center, Kitakami, Japan. nurasaki@sv02.ac.pref.iwate.jp
N
N
Urasaki
M
M
Tokumoto
R
R
Terauchi
K
K
Tarora
I
I
Chinen
Y
Y
Ban
T
T
Kayano
H
H
Tanaka
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism*; D020224:Expressed Sequence Tags; D019204:GTP-Binding Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010940:Plant Proteins; D016133:Polymerase Chain Reaction; D019685:Rosales / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D020693:rab2 GTP-Binding Protein / Q000737:chemistry
D018076:DNA, Complementary; C424085:PGP1 protein, Carica papaya; D010940:Plant Proteins; D019204:GTP-Binding Proteins; D020693:rab2 GTP-Binding Protein