A preliminary study of beta-cyclodextrin/metoprolol tartrate inclusion complex for potential enantiomeric separation.
The inclusion complex formation between Metoprolol tartarata (MeT) and beta-cyclodextrin (beta-CD) has been investigated using hyperchromic shift at lambda(max) 274.4 nm of MeT. Different parameters such as stirring time, solvent composition (aqueous and aqueous/methanol solutions with methanol content up to 50%), pH values 4.0 and 8.0 were established for optimal inclusion complex formation and confirmed two stoichiometric compositions 1:1 and 1:2. Preliminary data on usage of MeT/beta-CD complex in reversed-phase HPLC indicate the potential application of this complex as a kind of pre-column derivatization for enantiomeric separation of beta(1)-blockers.
Radulovic
D M
DM
Faculty of Pharmacy, Institute of Pharmaceutical Chemistry and Drug Analysis, University of Belgrade, Vojvode Stepe 450, 11221 Belgrade, Yugoslavia.
Karljikovic-Rajic
K D
KD
Lucic
B M
BM
Vujic
Z B
ZB
D002851:Chromatography, High Pressure Liquid; D003505:Cyclodextrins / Q000737:chemistry* / Q000302:isolation & purification; D008790:Metoprolol / Q000737:chemistry* / Q000302:isolation & purification; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D047392:beta-Cyclodextrins
D003505:Cyclodextrins; D047392:beta-Cyclodextrins; D008790:Metoprolol; C031215:betadex