[Human tumor xenografted into SCID mice and human immune function reconstitution].
OBJECTIVE
In order to observe (1) The behavior of growth and metastasis of PG and PGPTS7 in SCID mice and human immune function reconstituted mice; (2) The ability of interleukin-6 autosecreted from PGTS7 in enhancing the anti-tumor activity of the peripheral blood lymphocytes (PBL).


METHODS
Xenografting of PG and PGTS7 into the subepithelial space of the SCID mice, and in some of these animals, human PBL were administrated simultaneously into the peritoneal cavities. The latent period, taken rate, growth speed, volume of the grafted tumor, incidences of metastasis in lungs and lymph nodes and serum level of human immunoglobulin of the immunity reconstituted mice were examined.


RESULTS
Although tumor growth had been detected in all the experimental animals, the latent period of grafted PGTS7 was postponed and the volume of tumor mass as well as the incidence of lymph node metastasis all became lower in the immunity reconstituted mice accompanied simultaneously with a higher serum level of human immunoglobulin (HIg).


CONCLUSIONS
(1) The SCID mice are good as an appropriated hosts in studying the behavior of growth and metastasis of PG and PGTS7. (2) IL-6 autosecreted from PGTS7 stimulates the proliferation and promotes the activating of PBL. It seems also able to enhance the liberation of human immunoglobulin: to suppress the growth of tumor cells and to reduced the rate of lymph node metastasis.
Department of Pathology, Beijing Medical University, Beijing 100083.
S
S
Wang
J
J
Zheng
J
J
Fu
D000818:Animals; D018286:Carcinoma, Giant Cell / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007116:Immunization, Passive; D007136:Immunoglobulins / Q000097:blood; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D008175:Lung Neoplasms / Q000276:immunology* / Q000473:pathology; D008207:Lymphatic Metastasis; D017710:Lymphocyte Transfusion; D051379:Mice; D008819:Mice, Nude; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D014162:Transfection; D014407:Tumor Cells, Cultured
D007136:Immunoglobulins; D015850:Interleukin-6