Activation of Ty transposition by mutagens.
The induction of Ty1 transposition by mutagens (MMS and 4NQO) in asynchronous cultures and cells blocked in G1 and G2/M suggested G1 dependence of activation of Ty1 element by DNA damage. Northern blot analysis revealed immediate five-fold increase in levels of Ty1 transcript after 20min incubation of cells with 1 microg/ml 4NQO and four-fold increase in Ty1 RNA after treatment the cells with 0.1% MMS. Western blot analysis showed no difference in TyA protein in treated and untreated with mutagen cells. Quantitative mutagenicity assay and Northern blot analysis demonstrated dependence of induction of Ty1 element by DNA-damaging agents on the function of RAD9 gene and independence on DUN1 gene.
Department of Cell Biology, Room 698, School of Medicine, 550 First Avenue, New York University, New York, NY 10016, USA. stale101@popmail.med.nyu.edu
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Staleva Staleva
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Venkov
D015112:4-Nitroquinoline-1-oxide / Q000633:toxicity*; D001483:Base Sequence; D017931:DNA Primers; D004251:DNA Transposable Elements; D016193:G1 Phase; D015151:Immunoblotting; D008741:Methyl Methanesulfonate / Q000633:toxicity*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects / Q000235:genetics
D017931:DNA Primers; D004251:DNA Transposable Elements; D009153:Mutagens; D015112:4-Nitroquinoline-1-oxide; D008741:Methyl Methanesulfonate