[Use of PCR for the identification of representatives of Lactobacillus and Streptococcus].
REP- and ERIC-PCR genome analysis of lactic acid bacteria of genera Lactobacillus and Streptococcus have shown the presence of REP- and ERIC-repetitive sequences with high degree of homology. Amplification products which separation in agar gel results in formation of a specific fingerprint are obtained under REP- and ERIC-PCR. It is shown that REP- and ERIC-specific primers can be used for PCR identification of both enterobacteria and lactic acid bacteria isolated from different ecological niches.
Institute of Microbiology and Virology, Ukrainian National Academy of Sciences, 154 Zabolotny St., Kyiv, 03143, Ukraine.
N K
NK
Kovalenko
L N
LN
Bur'ianovskiĭ
V S
VS
Podgorskiĭ
D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid; D013291:Streptococcus / Q000145:classification* / Q000235:genetics
D004269:DNA, Bacterial