High-performance liquid chromatographic determination of oxolinic acid and flumequine in the live fish feed artemia.
A high-performance liquid chromatography (HPLC) analytical method for the determination of oxolinic acid and flumequine in Artemia nauplii is described. The samples were extracted and cleaned up by a solid-phase extraction (SPE) procedure using SPE C18 cartridges. Oxolinic acid and flumequine were determined by reversed-phase HPLC using a mobile phase of methanol-0.1 M phosphate buffer, pH 3 (45:55, v/v) and a UV detection wavelength of 254 nm. Calibration curves were linear for oxolinic acid in the range of 0.2-50 microg/g (r2=0.9998) and for flumequine in the range of 0.3-50 microg/g (r2=0.9994). Mean recoveries amounted to 100.8% and 98.4% for oxolinic acid and flumequine, respectively. The quantification limit was 0.2 microg/g for oxolinic acid and 0.3 microg/g for flumequine. Quantitative data from an in vivo feeding study indicated excellent uptake of both drugs by Artemia nauplii.
Touraki
M
M
Department of Genetics, Development and Molecular Biology, School of Biology, Faculty of Sciences, Aristotle University of Thessaloniki, Greece. touraki@bio.auth.gr
Ladoukakis
M
M
Prokopiou
C
C
D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis*; D001156:Artemia / Q000737:chemistry*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D024841:Fluoroquinolones; D010093:Oxolinic Acid / Q000032:analysis*; D011807:Quinolizines / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D011807:Quinolizines; D010093:Oxolinic Acid; C012976:flumequine