Homodimeric monomethine cyanine dyes as fluorescent probes of biopolymers.
The fluorescence properties of newly synthesized homodimeric monomethine cyanine dyes in the presence of biopolymers are investigated. They do not fluoresce in TE buffer and bidistilled water but become strongly fluorescent (Q(F)=0.3-0.9) in the region 530-650 nm when bound to dsDNA and ssDNA. The detection limit of dsDNA is about 1.7 ng/ml. Some of dyes studied are able to distinguish between dsDNA and ssDNA, RNA, BSA in solution and gel electrophoresis. The influence of different factors (temperature, pH and viscosity of the medium, presence of histone) on the formation of the dye-biopolymer complexes is investigated. The results of steady-state and dynamic fluorescence measurements concerning the different types of binding between dyes and biopolymers show that the new dyes are applicable in molecular biology as highly sensitive fluorescence labels.
Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Sofia, Bulgaria. iltim@orgchm.bas.bg
I
I
Timtcheva
V
V
Maximova
T
T
Deligeorgiev
N
N
Gadjev
K H
KH
Drexhage
I
I
Petkova
D000818:Animals; D001704:Biopolymers / Q000737:chemistry*; D002232:Carbocyanines / Q000737:chemistry; D004247:DNA / Q000032:analysis; D004277:DNA, Single-Stranded / Q000032:analysis; D005456:Fluorescent Dyes / Q000737:chemistry*; D010777:Photochemistry; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry
D001704:Biopolymers; D002232:Carbocyanines; D004277:DNA, Single-Stranded; D005456:Fluorescent Dyes; D004247:DNA