Protein phosphatase 2A: identification in Oryza sativa of the gene encoding the regulatory A subunit.
A 2225 bp cDNA, designated RPA1, was isolated from an Oryza sativa cDNA library. Analysis revealed a 1761 bp coding sequence with 15 non-identical repeat units. The ORF encoded the A regulatory subunit of protein phosphatase 2A (PP2A-A) as ascertained by complementation of the yeast tpd3 mutant defective in this gene. The corresponding genomic DNA from a rice genome BAC library revealed that the gene contains eleven introns. The rice genome contains only a single copy of this gene as judged by Southern blot analysis. The PP2A protein is highly conserved in nature; the rice protein shows 88% amino acid identity with its counterparts in Arabidopsis or Nicotiana tabacum.
Yu
S
S
National Center For Gene Research, Chinese Academy of Sciences, Shanghai.
Lei
H
H
Chang
W
W
Söll
D
D
Hong
G
G
D000595:Amino Acid Sequence; D004247:DNA / Q000737:chemistry / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005091:Exons; D017343:Genes, Plant / Q000235:genetics*; D005816:Genetic Complementation Test; D007438:Introns; D008969:Molecular Sequence Data; D009154:Mutation; D012275:Oryza / Q000201:enzymology / Q000235:genetics*; D010749:Phosphoprotein Phosphatases; D010940:Plant Proteins / Q000235:genetics*; D054648:Protein Phosphatase 2; D012441:Saccharomyces cerevisiae / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D010940:Plant Proteins; D004247:DNA; D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2