In vitro production of peroxynitrite by haemocytes from marine bivalves: C-ELISA determination of 3-nitrotyrosine level in plasma proteins from Mytilus galloprovincialis and Crassostrea gigas.
BACKGROUND
Peroxynitrite is increasingly proposed as a contributor to defence system in marine bivalve. It can be formed by combination of superoxide and nitric oxide, and can react with tyrosine residues of proteins giving rise to 3-nitrotyrosine.


RESULTS
The present article describes a competitive ELISA for the measurement of 3-nitrotyrosine contents of plasma proteins from marine bivalves by means of a monoclonal anti 3-nitrotyrosine antibody mouse IgG.


CONCLUSIONS
This assay is sensitive enough to determine the amounts of 3-nitrotyrosine in plasma proteins from one animal only. Using the C-ELISA, we have shown that the phagocytosis of zymosan particles increased the 3-nitrotyrosine levels of plasma proteins from mussel M. galloprovincialis and oyster C. gigas 5.8 and 7.5 times respectively.
UMR 5098, CP 80, Université Montpellier II, 2 place E. Bataillon, 34095-Montpellier Cedex 5, France. jtorreil@univ-montp2.fr
J
J
Torreilles
B
B
Romestand
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D049872:Bivalvia / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D001798:Blood Proteins / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards; D006434:Hemocytes / Q000378:metabolism*; D010125:Ostreidae / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D030421:Peroxynitrous Acid / Q000096:biosynthesis*; D010587:Phagocytosis; D012015:Reference Standards; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D001798:Blood Proteins; D030421:Peroxynitrous Acid; C002744:3-nitrotyrosine; D014443:Tyrosine