Poly(ADP-ribose) polymerase-1 is required for efficient HIV-1 integration.
Poly(ADP-ribose) polymerase-1 (PARP-1; EC ) is an abundant nuclear enzyme, activated by DNA strand breaks to attach up to 200 ADP-ribose groups to nuclear proteins. As retroviral infection requires integrase-catalyzed DNA strand breaks, we examined infection of pseudotyped HIV type I in fibroblasts from mice with a targeted deletion of PARP-1. Viral infection is almost totally abolished in PARP-1 knockout fibroblasts. This protection from infection reflects prevention of viral integration into the host genome. These findings suggest a potential for PARP inhibitors in therapy of HIV type I infection.
Department of Neuroscience, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
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Juluri
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Zhou
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Leung
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Hermankova
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Snyder
D000818:Animals; D002460:Cell Line; D049452:Green Fluorescent Proteins; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics / Q000502:physiology*; D016662:Virus Integration
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins; D011065:Poly(ADP-ribose) Polymerases