Characterization of mouse GBP28 and its induction by exposure to cold.
OBJECTIVE
To investigate whether the expression of the novel adipose tissue-specific protein GBP28 in adipose tissue and serum are altered in mice under a variety of conditions.


METHODS
Mice were fed a high-fat diet for 4 weeks, fasted for 48 h or exposed at 4 degrees C.


METHODS
C57BL/6J mouse, male, 4--6 weeks old.


METHODS
GBP28 mRNA, GBP28 protein, blood glucose, insulin and fad pad weight of the mice.


RESULTS
We first confirmed that the mouse has GBP28 and its characteristics are the same as human GBP28. Serum concentration and mRNA levels of GBP28 significantly increased in the mice exposed to cold.


CONCLUSIONS
GBP28 may play a role in homeostasis, regulating body temperature and basal metabolic rate in response to changing environmental conditions. International Journal of Obesity (2001) 25, 75-83
Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
M
M
Yoda
Y
Y
Nakano
T
T
Tobe
S
S
Shioda
N H
NH
Choi-Miura
M
M
Tomita
D052242:Adiponectin; D000273:Adipose Tissue / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001481:Basal Metabolism; D001786:Blood Glucose / Q000032:analysis; D015152:Blotting, Northern; D015153:Blotting, Western; D001833:Body Temperature Regulation / Q000235:genetics*; D003080:Cold Temperature; D004041:Dietary Fats / Q000008:administration & dosage*; D004591:Electrophoresis, Polyacrylamide Gel; D005215:Fasting; D005508:Food Deprivation / Q000502:physiology; D006706:Homeostasis; D007150:Immunohistochemistry; D007328:Insulin / Q000097:blood; D036341:Intercellular Signaling Peptides and Proteins; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000378:metabolism; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics* / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis
D052242:Adiponectin; D001786:Blood Glucose; D004041:Dietary Fats; D007328:Insulin; D036341:Intercellular Signaling Peptides and Proteins; D011506:Proteins; D012333:RNA, Messenger