[A study on the relationship among activity of PDGFBB autocrine loop, cell proliferation and apoptosis in human glioma cells].
OBJECTIVE
To investigate the relationship between activity of autocrine loop of platelet-derived growth factor BB (PDGFBB), cell proliferation and apoptosis in gliomas.


METHODS
73 human gliomas with different degrees of malignancy were studied using in situ hybridization, in situ cell death detection (TUNEL method) and immunohistochemistry.


RESULTS
The activity of PDGFBB autocrine loop and cell proliferation in gliomas correlated positively with each other and increased with the degree of tumor malignancy. Apoptosis decreased with the increase of malignancy and correlated negatively with the activity of PDGFBB autocrine loop as well as proliferation in glioma cells.


CONCLUSIONS
These results suggest that all the above parameters have referential value in the evaluation of biological behavior of gliomas. Abnormal increase of activity of PDGFBB autocrine loop in glioma cells may be an important factor which stimulates cell proliferation and inhibits cell apoptosis and plays an important role in the development and progression of gliomas.
Department of Neuropathology, Neurology Institute, Tianjin Medical University Hospital, Tianjin 300052.
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D000293:Adolescent; D000328:Adult; D017209:Apoptosis; D001254:Astrocytoma / Q000378:metabolism / Q000473:pathology; D019898:Autocrine Communication; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D002455:Cell Division; D002648:Child; D002675:Child, Preschool; D005260:Female; D005909:Glioblastoma / Q000378:metabolism / Q000473:pathology; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D020574:Proto-Oncogene Proteins c-sis / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000378:metabolism; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000378:metabolism
D020574:Proto-Oncogene Proteins c-sis; D012333:RNA, Messenger; D020796:Receptor, Platelet-Derived Growth Factor alpha; D020797:Receptor, Platelet-Derived Growth Factor beta