A comparative study of Euro-Collins, low potassium University of Wisconsin and cold modified blood solutions in lung preservation in acute autotransplantations in the pig.
OBJECTIVE
The aim of the study was to assess the quality of lung preservation offered by Euro-Collins solution (EC), Cold Modified Blood solution (CMB) and low potassium University of Wisconsin solution (UWLP).


METHODS
Fifteen right lung auto-transplantations (five for each solution) in the pig (Large White) were performed after 2 h of cold ischaemic storage in physiological solution at 4 degrees C. Right lung biopsies were performed before ischaemia and 30 min after reperfusion, for histoenzymatic, histopathological and electron microscope studies.


RESULTS
After reperfusion, significant alterations were observed in the haemodynamics with only the right lung perfused; pulmonary arteriolar resistance increased by a factor of 5 in the EC group, by a factor of 4 in the CMB group and by a factor of 1.2 in the UWLP group; the right ventricular ejection fraction fell by 60% in the EC group, by 50% in the CMB group and by 31% in the UWLP group. Haemodynamic impairment was lower in the UWLP group (P<0.05; P<0.001) as was ischaemic-reperfusion injury (P<0.05). Oedema was observed in the EC group and extensive alveolar wall damage in the CMB group. Hypoxaemia was observed in all groups but the differences in the degree of hypoxaemia were not significant.


CONCLUSIONS
The authors concluded that UWLP solution was the most effective of the three in this transplant model.
Divisi
D
D
Department of Cardio-thoracic Surgery, Cardiologique Hospital, Claude Bernard-Lyon I University, 59 Boulevard Pinel, 69003, Lyon, France. dudivisi@tin.it
Montagna
P
P
Jegaden
O
O
Giusti
L
L
Berti
A
A
Coloni
G F
GF
Ricci
C
C
Mikaeloff
P
P
D000704:Analysis of Variance; D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D004195:Disease Models, Animal; D004638:Emergency Treatment; D005260:Female; D006439:Hemodynamics; D016040:Lung Transplantation / Q000379:methods*; D008297:Male; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D011336:Probability; D011897:Random Allocation; D015427:Reperfusion Injury / Q000453:epidemiology; D012129:Respiratory Function Tests; D018570:Risk Assessment; D012680:Sensitivity and Specificity; D013552:Swine; D014182:Transplantation, Autologous
D019188:Organ Preservation Solutions