Isolation and characterisation of genomic and cDNA clones coding for a serine-, alanine-, and proline-rich protein of Trypanosoma cruzi.
We report here the isolation and characterisation of genomic and cDNA clones encoding a Serine-, Alanine-, and Proline-rich protein (SAP) of Trypanosoma cruzi metacyclic trypomastigotes. The deduced peptides translated from these clones were characterised by a high content of residues of alanine, proline, serine, glycine, valine, and threonine distributed in several repeats: P(2-4), S(2-3), A(2-3), AS, SA, PA, AP, SP, PS, and TP. The repeats are partially homologous to the serine-, alanine-, and proline-containing motifs of Leishmania major and Leishmania mexicana proteophosphoglycans. Genes coding for SAP are part of a polymorphic family whose members are linked to members of gp85/sialidase and mucin-like gene families. This is consistent with the hypothesis that this genetic organisation could be a means by which T. cruzi co-ordinates the expression of major surface proteins.
Escola Paulista de Medicina, UNIFESP, Departamento de Microbiologia, Imunologia e Parasitologia, R. Botucatu, 862, CEP 04023-062, S. Paulo, Brazil.
M S
MS
Carmo
M R
MR
Santos
L M
LM
Cummings
J E
JE
Araya
L M
LM
Yamauchi
N
N
Yoshida
R A
RA
Mortara
J
J
Franco da Silveira
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D018503:Genome, Protozoan; D008969:Molecular Sequence Data; D005810:Multigene Family; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014349:Trypanosoma cruzi / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
D018076:DNA, Complementary; D015800:Protozoan Proteins; C431649:SAP protein, Trypanosoma cruzi