[Lymph node mapping and axillary sentinel lymph node biopsy in 243 invasive breast cancers with no palpable nodes. The south Lyon hospital center experience].
OBJECTIVE
To evaluate the effect of intraoperative lymph node mapping and sentinel lymph node dissection (SLND) on the axillary staging of patients with N0 breast carcinoma. Two techniques were used: blue dye alone (Evans Blue and Patent Blue) and combined technique (blue dye and isotope).


METHODS
The incidence of axillary node metastasis in axillary lymph node dissection (ALND) and SLND was compared prospectively. Multiple sections of each SLN were examined by HPS staining and immunohistochemical techniques. Two sections of each non sentinel node in ALND specimens were examined by routine HPS staining.


RESULTS
243 patients underwent ALND after SLN biopsy. The SLN detection rate was 225/243 cases (92.59%): 89.94% with blue dye alone and 100% with the combined technique. The false-negative rate was less than 2%.


CONCLUSIONS
SN biopsy is an accurate staging technique for N0 breast cancer. SLN biopsy with multiple sections and immunohistochemical staining of the SLN can identify significantly more patients with lymph node metastases than ALND with routine HPS staining.
Bobin
J Y
JY
Département de chirurgie oncologique, centre hospitalier Lyon-Sud, 69495 Pierre-Bénite, France. bobin@chu-lyon.fr
Spirito
C
C
Isaac
S
S
Zinzindohoue
C
C
Joualee
A
A
Khaled
M
M
Perrin-Fayolle
O
O
D001706:Biopsy; D001943:Breast Neoplasms / Q000473:pathology* / Q000601:surgery; D004396:Coloring Agents; D005070:Evans Blue; D005188:False Negative Reactions; D005260:Female; D005602:France; D006801:Humans; D007150:Immunohistochemistry; D007430:Intraoperative Care / Q000379:methods* / Q000592:standards; D008408:Mastectomy; D009367:Neoplasm Staging / Q000379:methods* / Q000592:standards; D011446:Prospective Studies; D012394:Rosaniline Dyes; D021701:Sentinel Lymph Node Biopsy / Q000379:methods* / Q000592:standards
D004396:Coloring Agents; D012394:Rosaniline Dyes; D005070:Evans Blue; C008769:sulfan blue