Enhanced strand break induction of DNA by resonant metal-innershell photoabsorption.
We determined the number of single and double strand breaks (ssb and dsb) in a DNA-chloroterpyridine platinum complex induced by resonant photoabsorption in the L(III) innershell of a platinum atom. The number of ssb and dsb were measured in supercoiled plasmids (AG30) versus the chloroterpyridine platinum concentration, i.e., the ratio of intercalated molecules to the number of phosphate sites in DNA. A significant increase in the number of ssb and dsb was observed when the DNA contained intercalated molecules. This technique is an efficient way to induce ssb and dsb triggered by the atomic Auger effect.
Laboratoire des Collisions Atomiques et Moléculaires (URA 281), Orsay, France. lesech@lcam.u-psud.fr
C
C
Le Sech
K
K
Takakura
C
C
Saint-Marc
H
H
Frohlich
M
M
Charlier
N
N
Usami
K
K
Kobayashi
D000465:Algorithms; D004249:DNA Damage / Q000528:radiation effects*; D004278:DNA, Superhelical / Q000528:radiation effects; D004587:Electrophoresis, Agar Gel; D007364:Intercalating Agents; D008670:Metals; D010755:Organophosphates / Q000528:radiation effects; D009944:Organoplatinum Compounds; D017785:Photons; D010957:Plasmids / Q000528:radiation effects
D004278:DNA, Superhelical; D007364:Intercalating Agents; D008670:Metals; D010755:Organophosphates; D009944:Organoplatinum Compounds; C021975:chloroterpyridineplatinum(II)