A novel method for the determination of total protein in human serum by near infrared fluorescence recovery.
A novel fluorometric method has been developed for the determination of total protein in human serum with a new near-IR reagent as a fluorescence probe, based on the fluorescence recovery of the cyanine-CTAB system in the presence of protein. Maximum fluorescence is produced with maximum excitation and emission wave-lengths at 765 and 812 nm, respectively. Under optimal conditions, the calibration graphs are linear over the range 0.4-12.0 microg/mL for protein. The detection limit is 70 ng/mL, and the relative standard deviation of six replicate measurements is 1.14% for 6.0 microg/mL protein. The results are satisfactory.
Zheng
H
H
Department of Chemistry, the Key Laboratory of Analytical Sciences of MOE, Xiamen University, PR China.
Zhu
C Q
CQ
Li
D H
DH
Chen
Q Y
QY
Yang
H H
HH
Chen
X L
XL
Xu
J G
JG
D000818:Animals; D001798:Blood Proteins / Q000032:analysis*; D002138:Calibration; D002232:Carbocyanines / Q000737:chemistry; D002417:Cattle; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007259:Infrared Rays; D015335:Molecular Probes; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000032:analysis; D013050:Spectrometry, Fluorescence / Q000379:methods / Q000592:standards; D013501:Surface-Active Agents / Q000737:chemistry
D001798:Blood Proteins; D002232:Carbocyanines; D002593:Cetrimonium Compounds; D005456:Fluorescent Dyes; D015335:Molecular Probes; D013501:Surface-Active Agents; D012710:Serum Albumin, Bovine; D000077286:Cetrimonium