[Investigations on the usefulness of the dry chemistry blood anaylsis system SPOTCHEM SP-4410in laboratory diagnosis of cattle].
The usefulness of the dry-chemistry blood analyzer, SPOTCHEM SP-4410, for analysis of bovine blood chemistry was studied in a veterinary clinic. The control serum Precipath-U, Boehringer-Mannheim, was used to measure precision within each run and between days. The coefficients of variation (CV) ranged between 1.54% and 4.86%, with the exception of albumin and creatine phosphokinase showing a CV of 6.3% and 10.03% for between-day precision. For methodological comparison bovine serum samples were assayed with both the SPOTCHEM SP-4410 and the automated blood analyzer HITACHI 705, which served as a wet-chemistry reference system. The following analytes were measured: glucose, urea, creatinine, total protein, albumin, total bilirubin and the enzymes AST, CPK and gamma-GT. For hemoglobin, which was measured in heparinized whole blood, the CO oximeter 855, CIBA-CORNING, was used as a reference system. The comparative analysis showed very good correlation in eight of ten parameters and their correlation coefficients (r) ranged between 0.962 and 0.998. Only the correlation coefficients of the analysis of total bilirubin (r = 0.903) and albumin (r = 0.771) were less satisfactory. The recovery test was carried out with the two parameters glucose and blood urea. The recovery of glucose was 93.7% and of urea 98.8%. The SPOTCHEM SP-4410 is easy to use and proved to be reliable and accurate, and therefore it seems to be useful for analysis of bovine blood samples.
II. Medizinischen Tierklinik (Lehrstuhl für Innere Medizin und Chirurgie der Wiederkäuer, Ludwig-Maximilians-Universität München.
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Lorenz
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Aigner
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Klee
D000818:Animals; D001322:Autoanalysis / Q000295:instrumentation / Q000379:methods; D001774:Blood Chemical Analysis / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*; D001786:Blood Glucose / Q000032:analysis; D001798:Blood Proteins / Q000032:analysis; D001800:Blood Specimen Collection / Q000379:methods / Q000662:veterinary; D002417:Cattle / Q000097:blood*; D004798:Enzymes / Q000097:blood; D006454:Hemoglobins / Q000032:analysis; D015203:Reproducibility of Results
D001786:Blood Glucose; D001798:Blood Proteins; D004798:Enzymes; D006454:Hemoglobins