Analysis of phytochelatins in plant matrices by pre-column derivatization, high-performance liquid chromatography and fluorescence-detection.
A sensitive method for the determination of phytochelatins in plant matrices by pre-column derivatization with monobromobimane (mBrB) and high-performance liquid chromatography (HPLC) on reversed phases and fluorescence-detection has been developed and applied to cucumber sprouts (Cucumis sativus) treated with cadmium and to the water moss Fontinalis antipyretica (Cd in environmentally-relevant concentrations). Whereas phytochelatins were found in the Cd-treated sprouts, no phytochelatins were detected in Fontinalis anitipyretica.
Döring
S
S
Internationales Hochschulinstitut Zittau, Lehrstuhl Umweltverfahrenstechnik, Zittau, Germany.
Korhammer
S
S
Oetken
M
M
Markert
B
B
D019068:Bryopsida / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D018553:Cucumis sativus / Q000737:chemistry*; D005978:Glutathione; D008667:Metalloproteins / Q000032:analysis*; D008670:Metals / Q000032:analysis; D054811:Phytochelatins; D010940:Plant Proteins / Q000032:analysis*; D010944:Plants / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods / Q000592:standards; D014131:Trace Elements / Q000032:analysis
D008667:Metalloproteins; D008670:Metals; D010940:Plant Proteins; D014131:Trace Elements; D054811:Phytochelatins; D005978:Glutathione