Isolation and characterization of a black-pigmented Corynebacterium sp. from a woman with spontaneous abortion.
An unusual black-pigmented coryneform bacterium was isolated from the urogenital tract of a woman who experienced a spontaneous abortion during month 6 of pregnancy. Biochemical and chemotaxonomic analyses demonstrated that the unknown bacterium belonged to the genus Corynebacterium. Phylogenetic analysis based on 16S rRNA sequences (GenBank accession no. AF220220) revealed that the organism was a member of a distinct subline which includes uncultured Corynebacterium MTcory 1P (GenBank accession no. AF115934), derived from prostatic fluid, and Corynebacterium CDC B8037 (GenBank accession no. AF033314), an uncharacterized black-pigmented coryneform bacterium. On the basis of chemotaxonomic and phylogenetic evidence, this organism probably represents a new species and is most closely related to the uncharacterized Centers for Disease Control and Prevention group 4 coryneforms. Our strain is designated CN-1 (ATCC 700975).
Shukla
S K
SK
Department of Clinical Research, Marshfield Medical Research Foundation, 1000 North Oak Ave., Marshfield, Wisconsin 54449, USA. shuklas@mfldclin.edu
Vevea
D N
DN
Frank
D N
DN
Pace
N R
NR
Reed
K D
KD
D000022:Abortion, Spontaneous; D000328:Adult; D003352:Corynebacterium / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D003354:Corynebacterium Infections / Q000382:microbiology*; D004275:DNA, Ribosomal / Q000032:analysis; D005260:Female; D020459:Genes, rRNA / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D010860:Pigments, Biological / Q000378:metabolism*; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014621:Vagina / Q000382:microbiology
D004275:DNA, Ribosomal; D010860:Pigments, Biological; D012336:RNA, Ribosomal, 16S