Spatially and temporally regulated expression of myosin heavy chain alternative exons during Drosophila embryogenesis.
We used alternative exon-specific probes to determine the accumulation of transcripts encoding myosin heavy chain (MHC) isoforms in Drosophila melanogaster embryos. Six isoforms accumulate in body wall muscles. Transverse (external) muscles express a different major form than intermediate and internal muscles, suggesting different physiological properties. Cardioblasts express one of the somatic muscle transcripts; visceral muscles express at least two transcript types. The pharyngeal muscle accumulates a unique Mhc transcript, suggesting unique contractile abilities. Mhc transcription begins in stage 12 in visceral and somatic muscles, but as late as stage 15 in cardioblasts. This is the first study of myosin isoform localization during insect embryogenesis, and forms the basis for transgenic and biochemical experiments designed to determine how MHC domains regulate muscle physiology.
Zhang
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Department of Biology and Molecular Biology Institute, San Diego State University, San Diego, CA 92182-4614, USA.
Bernstein
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D017398:Alternative Splicing; D000818:Animals; D004330:Drosophila / Q000196:embryology*; D005091:Exons; D017403:In Situ Hybridization; D008957:Models, Genetic; D009132:Muscles / Q000378:metabolism; D009206:Myocardium / Q000166:cytology; D018995:Myosin Heavy Chains / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D010609:Pharyngeal Muscles / Q000166:cytology; D020033:Protein Isoforms; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors; D014158:Transcription, Genetic; D019076:Transgenes
D020033:Protein Isoforms; D012333:RNA, Messenger; D018995:Myosin Heavy Chains