[Detection of Mycoplasma genitalium in throat by nested polymerase chain reaction and analysis of DNA sequencing in pediatric patients with acute upper respiratory tract infections].
OBJECTIVE
To explore the relation between Mycoplasma genitalium (Mg) and acute upper respiratory tract infection in pediatric patients by using nested polymerase chain reaction(nPCR) and analysis of DNA sequencing techniques.


METHODS
The specimens of throat swab were obtained in 62 acute upper respiratory tract infections children and 80 health children. The two kinds of Mg species-specific, in which the sequences of I-III and V-VII variable regions of Mg 16Sr RNA gene were detected by using nPCR technique. DNA sequences of positive product were detected and analysed.


RESULTS
The Positive rate of health children is 5% (4/80), while the acute upper respiratory tract infection children is 21% (13/62). The difference is significant (P < 0.001). The DNA sequences of positive product shows that: there are two point mutation(T-->C) in the sequences of V-VII variable regions of throat swab of one pediatric patient, other sequences are same as those Mg type strain(G-37T).


CONCLUSIONS
Acute upper respiratory tract infection of pediatric patients have relation with Mg. There are Mg mutant strain in China.
Mi
Z
Z
Wuxi Clone Gen-Tech Institute, Wuxi, 214026 China.
Lu
Y
Y
Qin
L
L
D018829:Adhesins, Bacterial / Q000032:analysis; D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D006801:Humans; D007223:Infant; D009174:Mycoplasma / Q000235:genetics / Q000302:isolation & purification*; D009175:Mycoplasma Infections / Q000175:diagnosis*; D010614:Pharynx / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012141:Respiratory Tract Infections / Q000175:diagnosis*; D017422:Sequence Analysis, DNA
D018829:Adhesins, Bacterial; C073268:adhesin, Mycoplasma genitalium