[The rapid detection kit based on neuraminidase activity of influenza virus].
The ZstatFlu test(ZymeTx, USA) is a rapid detection kit for influenza types A and B virus. This test is based upon the reaction between viral neuraminidase from influenza viruses and chromogenic substrate. The positive specimen of influenza type A or B virus cleave the substrate and produce a blue colored product. The ZstatFlu was evaluated by a prototype viruses, isolated viruses and clinical specimens. At result, this kit was reactive for all human influenza type A and B virus. No cross reactivity was detected with other respiratory viruses, including parainfluenza type 1, 2, 3 and mumps viruses with neuraminidase activity. Throat swabs were used for the test. By comparison with cell culture and RT-PCR. The sensitivity and the specificity was 77.0% and 90.2% respectively. The ZstatFlu should be useful for the rapid diagnosis of influenza virus infection.
Kawasaki City Institute of Public Health.
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Shimizu
D006801:Humans; D009980:Influenza A virus / Q000201:enzymology / Q000302:isolation & purification*; D009981:Influenza B virus / Q000201:enzymology / Q000302:isolation & purification*; D009439:Neuraminidase / Q000032:analysis*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
D011933:Reagent Kits, Diagnostic; D009439:Neuraminidase