Characterization of a Sarcocystis neurona isolate from a Missouri horse with equine protozoal myeloencephalitis.
Little information is available about antigenic variation of Sarcocystis neurona isolated from horses with equine protozoal myeloencephalitis, nor is there much information available on the specific antibody pattern to S. neurona antigens of horses from different geographic regions where S. neurona isolates have been obtained. This communication reports on the characterization of a new S. neurona isolate, SN-MU1. The isolate was obtained from a 3-year old Thoroughbred that had asymmetrical neurological signs and localized skeletal muscle atrophy. This S. neurona isolate is similar to other S. neurona isolates by molecular analysis of the internal transcribed spacer (ITS-1) region and a random-amplified polymorphic DNA marker, but is phenotypically distinct from the other S. neurona isolates examined. Evaluation of the antibodies from the affected horse and immunohistochemical results suggested that antigenic variation of S. neurona can result in variable antibody-antigen reactivity observed in the S. neurona immunoblot test.
Marsh
A E
AE
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Connaway Hall, 1600 East Rollins Dr., Columbia, MO 65211, USA. marshae@missouri.edu
Johnson
P J
PJ
Ramos-Vara
J
J
Johnson
G C
GC
D000818:Animals; D000940:Antigenic Variation; D015153:Blotting, Western / Q000662:veterinary; D021901:DNA, Intergenic / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004679:Encephalomyelitis / Q000469:parasitology / Q000662:veterinary*; D005819:Genetic Markers; D006734:Horse Diseases / Q000469:parasitology*; D006736:Horses; D008297:Male; D009133:Muscular Atrophy / Q000469:parasitology / Q000662:veterinary; D010641:Phenotype; D019105:Random Amplified Polymorphic DNA Technique / Q000662:veterinary; D012522:Sarcocystis / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D012523:Sarcocystosis / Q000469:parasitology / Q000662:veterinary*
D021901:DNA, Intergenic; D005819:Genetic Markers