How enteral feeding options influence corticosterone patterns in rats.
Even with all the nutritional research conducted to date, it is not clear which enteral nutrition delivery and composition options are most physiologically sound. Glucocorticoid temporal patterns are reported to be shifted or disrupted with restricted feeding schedules, but because of intermittent sampling, temporal patterns have not been completely depicted. The purpose of this study was to characterize corticosterone temporal patterns while systematically varying selected enteral feeding options in a well-established nutritional animal model. A 2 x 2 x 2 x 2 randomized block experimental design was used in which enteral feeding schedules, delivery methods, kilocalorie levels (kcal), and fiber contents were systematically varied in rats (n = 80), and plasma corticosterone was measured by 125I radioimmunoassay. Blood samples were drawn hourly over 24 h. With cosinor analysis, 24-h and 12-h corticosterone rhythmic components were tested in each feeding group. Five of 16 feeding groups had a significant (p < or = 0.05) 24-h rhythmic component, and 3 more showed a trend (p > 0.05 < 0.10); 7 of these groups were on 24-h feeding schedules. When rhythmic components were detectable, groups receiving high-fiber formula displayed more uniform rhythm characteristics than did no-fiber groups. Only 1 group had a significant 12-h rhythmic component, and 1 showed a trend. Both were on 12-h, high-fiber restricted kcal feedings. In this small animal sample, no one enteral feeding option guaranteed a 24-h corticosterone pattern. The option coming closest was formula delivered on a 24-h schedule. This temporal pattern is one aspect to consider in enteral nutrition. The underlying mechanisms have yet to be elucidated.
Westfall
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Department of Population Based Nursing, Gerontology Division, Oregon Health Sciences University, USA.
D000704:Analysis of Variance; D000818:Animals; D001835:Body Weight; D002940:Circadian Rhythm; D003345:Corticosterone / Q000097:blood*; D004038:Diet, Reducing; D004043:Dietary Fiber / Q000008:administration & dosage; D002149:Energy Intake; D004750:Enteral Nutrition / Q000009:adverse effects / Q000379:methods*; D005526:Food, Formulated / Q000009:adverse effects / Q000032:analysis*; D008297:Male; D023421:Models, Animal; D011863:Radioimmunoassay; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D004043:Dietary Fiber; D003345:Corticosterone