[An experimental study on the capability of laminin receptor antibody and protein kinase C inhibitor to inhibit tumor metastasis in vivo].
OBJECTIVE
To study methods for blocking tumor metastasis.


METHODS
Endogenous laminin (LN), protein kinase C (PKC) inhibitor staurosporine (SP), anti-LN receptor (LN-R) were used to act on murine fibrosarcoma cells which were strongly positive for endogenous LN and LN-R, then infused into the caudal veins of 77 C57 mice once, to initiate lung tumor formation. Before infusion, the cells were all measured for surface LN-R quantity with flow cytometry.


RESULTS
The positive rate of LN-R on tumor cell surface in the control group, LN group, SP group, anti-LN-R group and anti-LN group being 56.5%, 76.3%, 50.2%, 31.9% and 20.6% respectively. The average volume of the tumors in the lungs of mice being 76 mm3, 110 mm3, 14 mm3, 5 mm3 and 20 mm3 respectively. The rate of tumor embolus formation in lymphatic and blood vessels being 81%, 94%, 36%, 43% and 68% respectively.


CONCLUSIONS
The pathological changes and metastasis in the LN group are more svere than that in control group. But all other groups are less severe than the control group. The level of LN-R on tumor cell surface is positively correlated with tumor volume and rat of tumor embolus formation. The lipositol conduction system in tumor cells is also closely related with size of tumor foci and rate of tumor embolus formation.
Department of Pathology, Jinan University Medical College, Guangzhou 510632.
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D000818:Animals; D005260:Female; D005354:Fibrosarcoma / Q000378:metabolism* / Q000473:pathology; D007797:Laminin / Q000494:pharmacology*; D008175:Lung Neoplasms / Q000378:metabolism / Q000556:secondary; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D011897:Random Allocation; D017474:Receptors, Laminin / Q000378:metabolism*; D019311:Staurosporine / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D007797:Laminin; D017474:Receptors, Laminin; D011493:Protein Kinase C; D019311:Staurosporine