Direct determination of 1-naphthoxylactic acid in biological fluids by non-protected fluid room temperature phosphorimetry.
A selective and sensitive room-temperature phosphorimetric method for the direct determination of 1-naphthoxylactic acid (NA) in biological fluids is described. It is based on obtaining a phosphorescence signal from NA using TlNO3 as a heavy atom perturber and Na2SO3 as a deoxygenator without a protective medium. This technique is named non-protected room-temperature phosphorescence (NP-RTP), which allows to determine analytes in complex matrices without the need for tedious prior separation. Optimization of the operational conditions resulted in a detection limit for NA of 9.6 ng/mL according to the error propagation theory. The repeatability and standard deviation were also determined. This method was successfully applied to the determination of NA in urine and human serum.
Murillo Pulgarín
J A
JA
Department of Analytical Chemistry and Food Technology, University Castilla-La Mancha, Ciudad Real, Spain. jamurill@qata-cr.uclm.es
Alañón Molina
A
A
D000319:Adrenergic beta-Antagonists / Q000097:blood / Q000652:urine; D005470:Fluorometry / Q000592:standards; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000032:analysis* / Q000097:blood / Q000652:urine; D008163:Luminescent Measurements; D011433:Propranolol / Q000097:blood / Q000652:urine; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000319:Adrenergic beta-Antagonists; D007773:Lactates; C002685:naphthoxylactic acid; D011433:Propranolol