Structural changes in rat Pneumocystis carinii surface antigens after terbinafine administration in experimental P. carinii pneumonia.
Terbinafine is a synthetic antifungal agent which has recently been found to be highly effective against Pneumocystis carinii. This study evaluated the efficacy of terbinafine on rat P. carinii antigenic profile and the immune response by Western blot analysis, in comparison with atovaquone and co-trimoxazole in rats with pneumocystosis. Terbinafine was shown to target two specific major antigens, particularly those of 116 and 35-40 kDa. Antibodies reactive against these moieties were found in all rats treated with atovaquone and co-trimoxazole, but not in those treated with terbinafine. These surface antigen modifications could be related to disease severity and could provide additional information for monitoring the efficacy of this treatment.
Section of Infectious Diseases, Department of Clinical and Experimental Medicine, University of Ferrara, via Fossato di Mortara 23, I-44100 Ferrara, Italy. cnc@ifeuniv.unife.it
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Angelici
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Canipari
D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D000946:Antigens, Fungal / Q000737:chemistry* / Q000187:drug effects; D053626:Atovaquone; D001769:Blood / Q000276:immunology / Q000382:microbiology; D015153:Blotting, Western; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology / Q000382:microbiology; D004195:Disease Models, Animal; D004586:Electrophoresis / Q000379:methods; D007165:Immunosuppression Therapy; D008297:Male; D009281:Naphthalenes / Q000494:pharmacology*; D009285:Naphthoquinones / Q000494:pharmacology; D011010:Pneumocystis / Q000187:drug effects / Q000276:immunology*; D011020:Pneumonia, Pneumocystis / Q000188:drug therapy* / Q000276:immunology / Q000401:mortality; D051381:Rats; D017207:Rats, Sprague-Dawley; D015996:Survival Rate; D000077291:Terbinafine; D015662:Trimethoprim, Sulfamethoxazole Drug Combination / Q000494:pharmacology
D000935:Antifungal Agents; D000946:Antigens, Fungal; D009281:Naphthalenes; D009285:Naphthoquinones; D015662:Trimethoprim, Sulfamethoxazole Drug Combination; D000077291:Terbinafine; D053626:Atovaquone