Characterization of the Aspergillus nidulans septin (asp) gene family.
Members of the septin gene family are involved in cytokinesis and the organization of new growth in organisms as diverse as yeast, fruit fly, worm, mouse, and human. Five septin genes have been cloned and sequenced from the model filamentous fungus A. nidulans. As expected, the A. nidulans septins contain the highly conserved GTP binding and coiled-coil domains seen in other septins. On the basis of hybridization of clones to a chromosome-specific library and correlation with an A. nidulans physical map, the septins are not clustered but are scattered throughout the genome. In phylogenetic analysis most fungal septins could be grouped with one of the prototypical S. cerevisiae septins, Cdc3, Cdc10, Cdc11, and Cdc12. Intron-exon structure was conserved within septin classes. The results of this study suggest that most fungal septins belong to one of four orthologous classes.
Momany
M
M
Department of Botany, University of Georgia, Athens, Georgia 30602, USA. momany@dogwood.botany.uga.edu
Zhao
J
J
Lindsey
R
R
Westfall
P J
PJ
D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000235:genetics* / Q000254:growth & development; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000235:genetics*; D018797:Cell Cycle Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D003598:Cytoskeletal Proteins / Q000235:genetics* / Q000502:physiology; D018076:DNA, Complementary / Q000378:metabolism; D016208:Databases, Factual; D005091:Exons; D005656:Fungal Proteins / Q000235:genetics*; D006160:Guanosine Triphosphate / Q000378:metabolism; D007438:Introns; D008954:Models, Biological; D008957:Models, Genetic; D008969:Molecular Sequence Data; D005810:Multigene Family; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D020161:Physical Chromosome Mapping; D051304:Profilins; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013997:Time Factors
C105655:AspB protein, Aspergillus nidulans; C099502:CDC11 protein, S cerevisiae; C099504:CDC12 protein, S cerevisiae; C086662:CDC3 protein, S cerevisiae; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D003598:Cytoskeletal Proteins; D018076:DNA, Complementary; D005656:Fungal Proteins; D051304:Profilins; D029701:Saccharomyces cerevisiae Proteins; D006160:Guanosine Triphosphate