Microdetermination of proteins using m-carboxychlorophosphonazo as detection probe by enhanced resonance light scattering spectroscopy.
Proteins can be determined using a common spectrofluorometer to detect the intensity of resonance light scattering (RLS). Under acidic conditions, the reaction between m-carboxychlorophosphonazo (CPA-mK) and proteins enhances the weak light scattering of CPA-mK drastically. This enhanced intensity is proportional to the concentration of proteins. The linear ranges for human serum albumin are 0.5-35.0 microg/mL, with detection limits of 0.104 microg/mL. The method yields results comparable to those of the calorimetric method using Coomassie Brilliant Blue G-250 (CBB) with relative standard deviations of 0.72-2.10% (n = 10). There is almost no interference by amino acids and most of the metal ions.
Department of Chemistry, Lanzhou University, PR China.
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Li
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Chen
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Zhang
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Xue
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Fan
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Hu
D000465:Algorithms; D001391:Azo Compounds / Q000737:chemistry*; D002138:Calibration; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008027:Light; D011506:Proteins / Q000032:analysis*; D012542:Scattering, Radiation; D012709:Serum Albumin / Q000737:chemistry; D012996:Solutions
D001391:Azo Compounds; D007202:Indicators and Reagents; D011506:Proteins; D012709:Serum Albumin; D012996:Solutions; C423510:m-carboxychlorophosphonazo