[Rapid assay for the assessment of a potential of chemical biocides to microbial destructors of industrial materials].
A colorimetric rapid assay for estimating the biocide potential of various chemicals towards metal biocorrosive and petroleum product degrading microbes was developed based on the reducing potential of live microbial cell. A water-soluble organic redox indicator, blue in the oxidized form and pink in the reduced form, was used as an indicator of the reducing potential of microbial cells. Once added to a suspension of vital microbial cells, it was reduced and changed in color. A good correlation between the results of this assay and viability control was obtained by employing surfactants and heavy metal ions.
State Research Center for Applied Microbiology, Obolensk, Moscow Oblast, 142279 Russia.
I A
IA
Novikov
B N
BN
Gurov
G V
GV
Shtuchnaia
V M
VM
Fomchenkov
V P
VP
Kholodenko
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D001681:Biological Assay; D002151:Calorimetry / Q000379:methods; D003343:Corrosion; D008670:Metals; D012680:Sensitivity and Specificity
D000900:Anti-Bacterial Agents; D008670:Metals