De novo synthesis and recycling pathways of sphingomyelin in rat Sertoli cells.
Sertoli cells from 19-day-old rats have two molecular species of sphingomyelin (SM1 and SM2) with different kinetic characteristics and fatty acid composition. Here, we have studied the incorporation of [14C]-choline and [14C]-palmitic acid into SM in presence or absence of fumonisin B1, an inhibitor of ceramide synthesis, and beta-chloroalanine, an inhibitor of sphinganine synthesis. The contributions of de novo synthesis and recycling pathways were estimated by analysis of the inhibition caused by these drugs. SM1 was synthesized more by sphingosine recycling, and SM2 was synthesized principally by ceramide recycling than SM1. De novo synthesis seems to be important for the two SM types, but our results showed that this pathway is more extensively utilized by SM2. In conclusion, using Sertoli cell cultures, we have shown for the first time that in the same cell different molecular species of SM are synthesized by different pathways.
Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
A L
AL
Ziulkoski
A R
AR
Zimmer
F C
FC
Guma
D000818:Animals; D002250:Carbon Radioisotopes; D002264:Carboxylic Acids / Q000494:pharmacology; D002478:Cells, Cultured; D002794:Choline / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D037341:Fumonisins; D008297:Male; D008954:Models, Biological; D019308:Palmitic Acid / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012708:Sertoli Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D013109:Sphingomyelins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015091:beta-Alanine / Q000031:analogs & derivatives* / Q000494:pharmacology
D002250:Carbon Radioisotopes; D002264:Carboxylic Acids; D004791:Enzyme Inhibitors; D037341:Fumonisins; D013109:Sphingomyelins; D015091:beta-Alanine; D019308:Palmitic Acid; C006977:3-chloroalanine; C056933:fumonisin B1; D002794:Choline