Glial cell line-derived neurotrophic factor in the substantia nigra from control and parkinsonian brains.
Glial cell line-derived neurotrophic factor (GDNF) was measured for the first time in the brain (substantia nigra, caudate nucleus, putamen, cerebellum, and frontal cortex) from control and parkinsonian patients by highly sensitive sandwich enzyme-linked immunosorbent assay. In both groups, the levels of GDNF in the various brain regions were lower (pg/mg protein) than those of brain-derived growth factor (ng/mg order), and were significantly higher in the nigro-striatal dopaminergic regions (substantia nigra, caudate nucleus, putamen) than in the cerebellum and frontal cortex (P < 0.05). However, the content of GDNF in the dopaminergic regions showed no significant difference between parkinsonian and control patients.
Department of Pharmacology, School of Dentistry, Aichi-Gakuin University, 464-8650, Nagoya, Japan.
M
M
Mogi
A
A
Togari
T
T
Kondo
Y
Y
Mizuno
O
O
Kogure
S
S
Kuno
H
H
Ichinose
T
T
Nagatsu
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019208:Brain-Derived Neurotrophic Factor; D002421:Caudate Nucleus / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D051100:Glial Cell Line-Derived Neurotrophic Factor; D006801:Humans; D008297:Male; D008875:Middle Aged; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010300:Parkinson Disease / Q000378:metabolism*; D011699:Putamen / Q000378:metabolism*; D018709:Statistics, Nonparametric; D013378:Substantia Nigra / Q000378:metabolism*
D019208:Brain-Derived Neurotrophic Factor; C495039:GDNF protein, human; D051100:Glial Cell Line-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; C019277:brain-derived growth factor