Supercritical fluid extraction of polycyclic aromatic hydrocarbons from liver samples and determination by HPLC-FL.
An extraction/clean-up procedure by SFE was developed for isolating PAHs from liver samples for subsequent HPLC-FL determination of ten PAHs in the enriched extract. Recoveries (90-115%) and RSD % (< or =7.7) were satisfactory. When applied to 11 samples of bird of prey (Tyto alba) protected species and classified of special interest, from the Galicia (Northwest to Spain), benzo[ghi]perylene and indeno[1,2,3-cd]pyrene were undetectable; chrysene and benzo[a]pyrene are only detected in one sample; benzo[a]anthracene and benzo[k]fluoranthene are only quantified in one sample and benzo[b]fluoranthene in two samples. The other PAHs, anthracene, fluoranthene and pyrene are present in almost all the samples.
Department of Analytical Chemistry, Nutrition and Bromatology, University of Santiago de Compostela, Spain.
S
S
González Amigo
M S
MS
García Falcón
M A
MA
Lage Yusty
J
J
Simal Lozano
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008099:Liver / Q000737:chemistry*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis* / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D013030:Spain; D020305:Strigiformes
D011084:Polycyclic Aromatic Hydrocarbons