The effect of zinc-crystallized glucagon-like peptide-1 on insulin secretion of macroencapsulated pancreatic islets.
This research investigates the use of an insulinotropic factor, glucagon-like peptide-1 (GLP-1), to enhance insulin secretion from islets within a macrocapsule. A zinc-crystallized form of GLP-1 was added to the macrocapsule device to have a longer and more controlled release of the bioactive monomer GLP-1. The type of macrocapsule device used for this study consisted of a hollow fiber (MWCO 100,000 and 1 mm inner diameter) containing rat islets and GLP-1 crystals within a poly(N-isopropylacrylamide-co-acrylic acid) (2 mol% acrylic acid) matrix. When incubating the system in media with a high glucose concentration (300 mg/dL), insulin secretion was enhanced with a >85% increase after an induction period. When the same type of system was used in a dynamic perfusion experiment, similar results were obtained. GLP-1 crystals can be an effective form to be entrapped in a bioartificial pancreas to enhance insulin secretion function, especially at high glucose concentrations.
Center for Controlled Chemical Delivery, Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Biomedical Polymer Research Building, Salt Lake City, Utah 84112, USA.
H
H
Gappa
M
M
Baudys
J J
JJ
Koh
S W
SW
Kim
Y H
YH
Bae
D000818:Animals; D002470:Cell Survival; D003460:Crystallization; D005934:Glucagon / Q000737:chemistry / Q000378:metabolism*; D052216:Glucagon-Like Peptide 1; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology / Q000378:metabolism*; D008297:Male; D019397:Pancreas, Artificial; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism*; D010477:Perfusion; D011108:Polymers / Q000737:chemistry; D011498:Protein Precursors / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015032:Zinc / Q000737:chemistry*
D007328:Insulin; D010446:Peptide Fragments; D011108:Polymers; D011498:Protein Precursors; D052216:Glucagon-Like Peptide 1; D005934:Glucagon; D015032:Zinc