Comparison of IsoCode STIX and FTA Gene Guard collection matrices as whole-blood storage and processing devices for diagnosis of malaria by PCR.
We compared two collection devices, IsoCode and FTA, with whole blood for the diagnosis of malaria by PCR (n = 100). Using whole blood as the reference standard, both devices were sensitive for the detection of single-species malaria infections by PCR (> or =96%). However, the detection of mixed infections was suboptimal (IsoCode was 42% sensitive, and FTA was 63% sensitive).
Centre for Travel and Tropical Medicine, Division of Infectious Diseases, Department of Medicine, Toronto General Hospital and the University of Toronto, 200 Elizabeth St., M5G 2C4 Toronto, Ontario, Canada.
K J
KJ
Zhong
C J
CJ
Salas
R
R
Shafer
A
A
Gubanov
R A
RA
Gasser
A J
AJ
Magill
J R
JR
Forney
K C
KC
Kain
D000818:Animals; D001800:Blood Specimen Collection / Q000295:instrumentation* / Q000379:methods; D016054:DNA, Protozoan / Q000097:blood*; D005374:Filtration; D006801:Humans; D016778:Malaria, Falciparum / Q000150:complications / Q000175:diagnosis* / Q000469:parasitology; D016780:Malaria, Vivax / Q000150:complications / Q000175:diagnosis* / Q000469:parasitology; D010963:Plasmodium falciparum / Q000235:genetics / Q000302:isolation & purification; D010966:Plasmodium vivax / Q000235:genetics / Q000302:isolation & purification; D012680:Sensitivity and Specificity
D016054:DNA, Protozoan