Effect of thiol drugs on the oxidative hemolysis in human erythrocytes.
The effect of different thiol drugs and 2-methyl-thiazolidine-2,4-dicarboxylic acid on the oxidative stress, induced by hydrogen peroxide, was examined in human erythrocytes. The results indicated that captopril (CA), methimazole, N-acetylcysteine (NAC), penicillamine and precursor of L-cysteine 2-methyl-thiazolidine-2,4-dicarboxylic acid (CP) might protect the erythrocyte membrane against lipid peroxidation in the experimental conditions. Captopril, methimazole and penicillamine had the strongest antioxidative properties at the concentration level of 0.5 mM. The protective effects gradually decreased at higher and lower concentrations of these drugs. Contrary, the antioxidative properties of N-acetylcysteine increased with its levels growing in the reaction mixture, and only N-acetylpenicillamine did not protect erythrocytes against oxidative damages. The effect of 2-methyl-thiazolidine-2,4-dicarboxylic acid showed in these in vitro experimental conditions that it could act as an antioxidant at the concentration as high as 5 mM and higher.
Institute of Medical Biochemistry, Jagiellonian University Collegium Medicum, Cracow, Poland.
M
M
Iciek
M
M
Polak
L
L
Włodek
D000111:Acetylcysteine / Q000494:pharmacology; D002216:Captopril / Q000494:pharmacology; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D008713:Methimazole / Q000494:pharmacology; D010396:Penicillamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D017382:Reactive Oxygen Species; D013438:Sulfhydryl Compounds / Q000494:pharmacology*
D017382:Reactive Oxygen Species; D013438:Sulfhydryl Compounds; D008713:Methimazole; C010005:N-acetylpenicillamine; D002216:Captopril; D010396:Penicillamine; D000111:Acetylcysteine