Nanoengineered structures for holding and manipulating liposomes and cells.
We describe the fabrication of nanoengineered holding pipets with concave seating surfaces and fine pressure control. These pipets were shown to exhibit exceptional stability in capturing, transporting, and releasing single cells and liposomes 1-12 microm in diameter, which opens previously inaccessible avenues of research. Three specific examples demonstrated the utility and versatility of this manipulation system. In the first, carboxyrhodamine was selectively incorporated into individual cells by electroporation, after which nearly all the medium (hundreds of microliters) surrounding the docked and tagged cells was rapidly exchanged (in seconds) and the cells were subsequently probed by laser-induced fluorescence (LIF). In the second study, a single liposome containing carboxyrhodamine was transported to a dye-free solution using a transfer pipet, docked to a holding pipet, and held firmly during physical agitation and interrogation by LIF. In the third study, pairs of liposomes were positioned between two microelectrodes, held in contact, and selectively electrofused and the resulting liposomes undocked intact.
Department of Chemistry, Stanford University, California 94305-5080, USA.
C F
CF
Wilson
G J
GJ
Simpson
D T
DT
Chiu
A
A
Strömberg
O
O
Orwar
N
N
Rodriguez
R N
RN
Zare
D002477:Cells / Q000737:chemistry*; D018274:Electroporation; D005453:Fluorescence; D007834:Lasers; D008081:Liposomes / Q000737:chemistry*; D008846:Micromanipulation / Q000295:instrumentation*
D008081:Liposomes