The role of nitrergic system in lidocaine-induced convulsion in the mouse.
The effects of N-nitro-L-arginine-methyl ester (L-NAME) a nitric oxide (NO) synthase inhibitor and L-arginine, a NO precursor, were investigated on lidocaine-induced convulsions. In the first experiment, four groups of mice received physiological saline (0.9%), L-arginine (300 mg/kg, i.p.), L-NAME (100 mg/kg, i.p.) and diazepam (2 mg/kg), respectively. Thirty minutes after these injections, all mice received lidocaine (50 mg/kg, i.p.). In the second experiment, four groups of mice received similar treatment in the first experiment, and 30 min after these injections, all mice received a higher dose of lidocaine (80 mg/kg). L-NAME (100 mg/kg, i.p.) and diazepam (2 mg/kg) significantly decreased the incidence of lidocaine (50 mg/kg)-induced convulsions. In contrast, the L-arginine treatment increased the incidence of lidocaine (80 mg/kg, i.p.)-induced convulsions significantly. These results may suggest that NO is a proconvulsant mediator in lidocaine-induced convulsions.
Department of Pharmacology, Faculty of Medicine, Ondokuz Mayis University, Samsun, Turkey. mehmet.kurt@turkport.com
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D000779:Anesthetics, Local / Q000633:toxicity*; D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology; D001120:Arginine / Q000494:pharmacology; D003975:Diazepam / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D008012:Lidocaine / Q000633:toxicity*; D008297:Male; D051379:Mice; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology*; D009569:Nitric Oxide / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D012640:Seizures / Q000139:chemically induced / Q000503:physiopathology*
D000779:Anesthetics, Local; D000927:Anticonvulsants; D004791:Enzyme Inhibitors; D009569:Nitric Oxide; D001120:Arginine; D008012:Lidocaine; D019001:Nitric Oxide Synthase; D003975:Diazepam; D019331:NG-Nitroarginine Methyl Ester