Expression of zebrafish Hoxa1a in neuronal cells of the midbrain and anterior hindbrain.
The expression pattern of zebrafish hoxa1a mRNA during embryonic development was studied. Herein, we show that hoxa1a mRNA is expressed in the ventral region of both the midbrain and anterior hindbrain during the developmental period from the pharyngula to the protruding-mouth stages via whole-mount in situ hybridization. Furthermore, double-labeling with anti-zHu antibody confirms that the zebrafish hoxa1a gene is expressed in neuronal cells. The observed temporal and spatial distributions of zebrafish hoxa1a mRNA differ greatly from the expression patterns of zebrafish hoxb1a and hoxb1b paralagous genes. In addition, in embryos injected with mouse ihh mRNA, hoxa1a-expressing cells increase in number with a dorsalized expression pattern in the midbrain.
Shih
L J
LJ
Institute of Zoology, Academia Sinica, Nankang 11529, Taiwan, Republic of, Taipei, China.
Tsay
H J
HJ
Lin
S C
SC
Hwang
S P
SP
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018398:Homeodomain Proteins / Q000096:biosynthesis*; D017403:In Situ Hybridization; D008636:Mesencephalon / Q000196:embryology*; D051379:Mice; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism; D012249:Rhombencephalon / Q000196:embryology*; D017386:Sequence Homology, Amino Acid; D013997:Time Factors; D014018:Tissue Distribution; D014157:Transcription Factors / Q000096:biosynthesis*; D015027:Zebrafish / Q000196:embryology*; D000099261:Homeobox A1 Protein
D018398:Homeodomain Proteins; D014157:Transcription Factors; D000099261:Homeobox A1 Protein