Production and physiological function of granulocyte colony-stimulating factor in non-pregnant human endometrial stromal cells.
Effects of granulocyte colony-stimulating factor (G-CSF) on proliferation and differentiation of normal human endometrial stromal cells were investigated in an in vitro decidualization culture of stromal cells. Unstimulated stromal cells secreted little prolactin and G-CSF, whereas 8-Br-cAMP-stimulated stromal cells secreted higher levels. There was no relationship, however, between the levels of prolactin and G-CSF secreted from the stimulated cells. Detectable levels of prolactin secretion were not found in two of six stromal cell cultures stimulated with 8-Br-cAMP; however, these two culture supernatants contained high concentrations of G-CSF. Co-stimulation of the stromal cells with 8-Br-cAMP and G-CSF enhanced prolactin secretion from the stimulated cells in a G-CSF concentration-dependent manner without any change in viable cell numbers. However, G-CSF did not affect prolactin secretion or viable cell numbers of 8-Br-cAMP-stimulated decidualized cells. These results indicate that G-CSF enhances cAMP-mediated decidualization of human endometrial stromal cells in an autocrine or paracrine fashion.
Department of Obstetrics and Gynecology, Osaka City University Medical School, Osaka, 1-4-3 Asahi-machi, Abeno-ku, Japan.
T
T
Tanaka
M
M
Miyama
M
M
Masuda
K
K
Mizuno
T
T
Sakamoto
N
N
Umesaki
S
S
Ogita
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D004717:Endometrium / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D016179:Granulocyte Colony-Stimulating Factor / Q000032:analysis / Q000096:biosynthesis / Q000502:physiology*; D006801:Humans; D007375:Interleukin-1 / Q000502:physiology; D011388:Prolactin / Q000378:metabolism / Q000502:physiology; D019713:Receptors, CCR5 / Q000378:metabolism; D016188:Receptors, Granulocyte Colony-Stimulating Factor / Q000378:metabolism; D017472:Receptors, Interleukin-1 / Q000378:metabolism; D017154:Stromal Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology
D007375:Interleukin-1; D019713:Receptors, CCR5; D016188:Receptors, Granulocyte Colony-Stimulating Factor; D017472:Receptors, Interleukin-1; D016179:Granulocyte Colony-Stimulating Factor; D015124:8-Bromo Cyclic Adenosine Monophosphate; D011388:Prolactin