OliI, a unique restriction endonuclease that recognizes the discontinuous sequence 5'-CACNN NGTG-3'.
A new type II restriction endonuclease designated OLI:I has been partially purified from the halophilic bacterium Oceanospirillum linum 4-5D. OLI:I recognizes the interrupted hexanucleotide palindrome 5'-CACNN NNGTG-3' and cleaves it in the center generating blunt-ended DNA fragments.
MBI Fermentas, Graiciuno 8, 2028 Vilnius, Lithuania and Institute of Biotechnology, Graiciuno 8, 2028 Vilnius, Lithuania.
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Padegimiene
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Maneliene
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Petruyte
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Janulaitis
D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D004262:DNA Restriction Enzymes / Q000302:isolation & purification / Q000378:metabolism*; D004795:Enzyme Stability / Q000187:drug effects; D020563:Gammaproteobacteria / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D011189:Potassium Chloride / Q000494:pharmacology; D013379:Substrate Specificity
D011189:Potassium Chloride; D004247:DNA; D004262:DNA Restriction Enzymes