Interleukin 1beta, interleukin-6, and tumor necrosis factor-alpha in endometriotic tissue and in endometrium.
OBJECTIVE
To compare the levels of IL-1beta, IL-6, and TNFalpha in endometriotic tissue and in endometrium from women with endometriosis and healthy controls.


METHODS
Open.


METHODS
Department of Obstetrics and Gynecology at a university hospital.


METHODS
Twenty-six women with endometriosis and 22 controls operated on for clinical indications.


METHODS
ELISA in homogenized tissue samples collected during surgery.


METHODS
Levels of IL-1beta, IL-6, and TNFalpha in tissue homogenates.


RESULTS
The three types of tissue differed significantly with respect to all three cytokines. Endometriotic tissue had significantly higher concentrations of IL-1beta than endometrium from both patients with endometriosis and healthy controls. Both endometriotic tissue and endometrium from patients had significantly higher concentrations of IL-6, and endometriotic tissue had significantly lower concentration of TNFalpha than did endometrium from controls. IL-1beta showed a cycle phase dependence that was significant in endometrium from patients, being higher in the secretory than in the follicular phase. IL-1beta was significantly higher in endometrioma than in lesions of other localizations. Concentrations of IL-1beta and IL-6 were positively correlated in endometriotic tissue and in endometrium from controls. No other significant correlations were found.


CONCLUSIONS
This study has shown a significant production of IL-1beta, IL-6, and TNFalpha in endometriotic tissue and endometrium, with significant differences between the tissue types, indicating a deviating cytokine pattern in both endometriotic tissue and endometrium from women with endometriosis compared with that from healthy controls.
Bergqvist
A
A
Department of Obstetrics and Gynecology, Huddinge University Hospital, and Karolinska Institutet, Stockhom, Sweden. agneta.bergqvist@obgyn.hs.sll.se
Bruse
C
C
Carlberg
M
M
Carlström
K
K
D004715:Endometriosis / Q000378:metabolism*; D004717:Endometrium / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis*; D015850:Interleukin-6 / Q000032:analysis*; D008597:Menstrual Cycle; D010049:Ovarian Diseases / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*
D007375:Interleukin-1; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha