Protein kinase D regulates the fission of cell surface destined transport carriers from the trans-Golgi network.
When a kinase inactive form of Protein Kinase D (PKD-K618N) was expressed in HeLa cells, it localized to the trans-Golgi network (TGN) and caused extensive tubulation. Cargo that was destined for the plasma membrane was found in PKD-K618N-containing tubes but the tubes did not detach from the TGN. As a result, the transfer of cargo from TGN to the plasma membrane was inhibited. We have also demonstrated the formation and subsequent detachment of cargo-containing tubes from the TGN in cells stably expressing low levels of PKD-K618N. Our results suggest that PKD regulates the fission from the TGN of transport carriers that are en route to the cell surface.
Biology Department, University of California, San Diego, La Jolla, CA 92093, USA.
M
M
Liljedahl
Y
Y
Maeda
A
A
Colanzi
I
I
Ayala
J
J
Van Lint
V
V
Malhotra
D001692:Biological Transport; D015704:CD4 Antigens / Q000378:metabolism; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology* / Q000378:metabolism; D002966:Clathrin / Q000378:metabolism; D020756:Coatomer Protein / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004721:Endoplasmic Reticulum / Q000378:metabolism; D011992:Endosomes / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005982:Glutathione Transferase / Q000378:metabolism; D006023:Glycoproteins; D006367:HeLa Cells; D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism; D008565:Membrane Proteins; D010766:Phosphorylation; D011233:Precipitin Tests; D011493:Protein Kinase C / Q000235:genetics / Q000502:physiology*; D021381:Protein Transport; D011994:Recombinant Proteins / Q000378:metabolism; D013696:Temperature; D013997:Time Factors; D014162:Transfection; D014759:Viral Envelope Proteins / Q000378:metabolism; D021601:trans-Golgi Network / Q000378:metabolism*
D015704:CD4 Antigens; D002966:Clathrin; D020756:Coatomer Protein; C028391:G protein, vesicular stomatitis virus; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D011994:Recombinant Proteins; C072237:TGOLN2 protein, human; D014759:Viral Envelope Proteins; D005982:Glutathione Transferase; C089212:protein kinase D; D011493:Protein Kinase C