Inhibition of caspase-1 induces cell death in pancreatic carcinoma cells and potentially modulates expression levels of bcl-2 family proteins.
Caspase-1 (interleukin-1beta-converting enzyme) is reported to play an important role in the regulation of apoptosis. We investigated the inhibition of caspase-1 by the cell permeable caspase-1 inhibitor Ac-AAVALLPAVLLALLAP-YVAD.CHO in pancreatic carcinoma cells. Inhibition of caspase-1 induced a non-apoptotic/"necrotic-like" cell death in AsPC-1, BxPC-3, MiaPaCa-2 and Panc-1 cells. Expression levels of bcl-2 and bax were up-regulated in caspase-1 inhibitor-treated cells while that of bcl-x(L) remained unaltered. Our observations support our previous findings that caspase-1 is potentially involved in anti-apoptotic processes in pancreatic carcinoma.
Division of Molecular Oncology, Department of General Surgery, University of Ulm, Steinhövelstrasse 9, 89075, Ulm, Germany.
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S
Schlosser
F
F
Gansauge
M
M
Ramadani
H G
HG
Beger
S
S
Gansauge
D017209:Apoptosis; D015153:Blotting, Western; D020170:Caspase 1 / Q000378:metabolism*; D061945:Caspase Inhibitors; D002453:Cell Cycle; D053938:DNA Fragmentation; D006801:Humans; D009336:Necrosis; D009842:Oligopeptides / Q000494:pharmacology; D010190:Pancreatic Neoplasms; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D015854:Up-Regulation; D051028:bcl-2-Associated X Protein
C423942:Ac-AAVALLPAVLLALLAPYVAD-CHO; C494826:BAX protein, human; D061945:Caspase Inhibitors; D009842:Oligopeptides; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D020170:Caspase 1