Ultrasonic depolymerization of the chitin-glucan complex from Aspergillus niger and antimutagenic activity of its product.
Sonication was effective for the depolymerization of carboxymethylated chitin-glucan complex (CM-CG) isolated from the cell wall of Aspergillus niger. After 10 min of sonication and subsequent gel filtration, two samples (CM-CG(I) and CM CG(II)) with significantly distinct molecular weights (660 kDa and 19 kDa, respectively) and different nitrogen contents (3.02 and 1.69%) were obtained. CM-CG(II) with lower Mw was also effective against cyclophosphamide mutagenicity by oral administration in mice.
Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovak Republic. machova@savba.sk
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E
Machová
G
G
Kogan
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D
Chorvatovicová
J
J
Sandula
D000818:Animals; D016587:Antimutagenic Agents / Q000138:chemical synthesis* / Q000494:pharmacology*; D001234:Aspergillus niger / Q000737:chemistry*; D002473:Cell Wall / Q000737:chemistry; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002686:Chitin / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003520:Cyclophosphamide / Q000037:antagonists & inhibitors / Q000633:toxicity; D005260:Female; D005936:Glucans / Q000737:chemistry*; D051379:Mice; D008813:Mice, Inbred ICR; D009153:Mutagens / Q000633:toxicity; D014462:Ultrafiltration; D014465:Ultrasonics
D016587:Antimutagenic Agents; D005936:Glucans; D009153:Mutagens; D002686:Chitin; D003520:Cyclophosphamide