The physical maps for sequencing human chromosomes 1, 6, 9, 10, 13, 20 and X.
We constructed maps for eight chromosomes (1, 6, 9, 10, 13, 20, X and (previously) 22), representing one-third of the genome, by building landmark maps, isolating bacterial clones and assembling contigs. By this approach, we could establish the long-range organization of the maps early in the project, and all contig extension, gap closure and problem-solving was simplified by containment within local regions. The maps currently represent more than 94% of the euchromatic (gene-containing) regions of these chromosomes in 176 contigs, and contain 96% of the chromosome-specific markers in the human gene map. By measuring the remaining gaps, we can assess chromosome length and coverage in sequenced clones.
Bentley
D R
DR
The Sanger Centre, Hinxton, Cambridge, UK. drb@sanger.ac.uk
Deloukas
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P
Dunham
A
A
French
L
L
Gregory
S G
SG
Humphray
S J
SJ
Mungall
A J
AJ
Ross
M T
MT
Carter
N P
NP
Dunham
I
I
Scott
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Ashcroft
K J
KJ
Atkinson
A L
AL
Aubin
K
K
Beare
D M
DM
Bethel
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Brady
N
N
Brook
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JC
Burford
D C
DC
Burrill
W D
WD
Burrows
C
C
Butler
A P
AP
Carder
C
C
Catanese
J J
JJ
Clee
C M
CM
Clegg
S M
SM
Cobley
V
V
Coffey
A J
AJ
Cole
C G
CG
Collins
J E
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Conquer
J S
JS
Cooper
R A
RA
Culley
K M
KM
Dawson
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E
Dearden
F L
FL
Durbin
R M
RM
de Jong
P J
PJ
Dhami
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PD
Earthrowl
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ME
Edwards
C A
CA
Evans
R S
RS
Gillson
C J
CJ
Ghori
J
J
Green
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L
Gwilliam
R
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Halls
K S
KS
Hammond
S
S
Harper
G L
GL
Heathcott
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RW
Holden
J L
JL
Holloway
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E
Hopkins
B L
BL
Howard
P J
PJ
Howell
G R
GR
Huckle
E J
EJ
Hughes
J
J
Hunt
P J
PJ
Hunt
S E
SE
Izmajlowicz
M
M
Jones
C A
CA
Joseph
S S
SS
Laird
G
G
Langford
C F
CF
Lehvaslaiho
M H
MH
Leversha
M A
MA
McCann
O T
OT
McDonald
L M
LM
McDowall
J
J
Maslen
G L
GL
Mistry
D
D
Moschonas
N K
NK
Neocleous
V
V
Pearson
D M
DM
Phillips
K J
KJ
Porter
K M
KM
Prathalingam
S R
SR
Ramsey
Y H
YH
Ranby
S A
SA
Rice
C M
CM
Rogers
J
J
Rogers
L J
LJ
Sarafidou
T
T
Scott
D J
DJ
Sharp
G J
GJ
Shaw-Smith
C J
CJ
Smink
L J
LJ
Soderlund
C
C
Sotheran
E C
EC
Steingruber
H E
HE
Sulston
J E
JE
Taylor
A
A
Taylor
R G
RG
Thorpe
A A
AA
Tinsley
E
E
Warry
G L
GL
Whittaker
A
A
Whittaker
P
P
Williams
S H
SH
Wilmer
T E
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Wooster
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R
Wright
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D002878:Chromosomes, Human, Pair 1; D002879:Chromosomes, Human, Pair 10; D002882:Chromosomes, Human, Pair 13; D002890:Chromosomes, Human, Pair 20; D002896:Chromosomes, Human, Pair 6; D020451:Contig Mapping; D015894:Genome, Human; D006801:Humans; D014960:X Chromosome