Alternate adenovirus type-pairs for a possible circumvention of host immune response to recombinant adenovirus vectors.
With the help of monoclonal antibodies the existence of at least 18 different earlier not known intertype (IT) specific epitopes were demonstrated in different numbers and combinations on the hexons of different adenovirus serotypes. The IT specific epitopes play an important role in the experimental gene therapy and in the recombinant adenovirus vaccination because of the harmful immune response of the recipient organisms directed against the many different epitopes of the adenovirus vector. For the elimination of harmful effect the authors suggest the use of multiple vectors, each prepared from different adenovirus serotypes showing the loosest antigenic relationship to each other. The vectors would be used sequentially when second or multiple administration is needed. For this purpose the authors determined and described 31 such adenovirus type-pairs, which are probably the best alternates for sequential use in experimental gene therapy.
Institute of Medical Microbiology, Faculty of General Medicine, Semmelweis University Budapest, Nagyvárad tér 4, H-1089 Budapest, Hungary.
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Lengyel
D000260:Adenoviruses, Human / Q000145:classification / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D002213:Capsid / Q000276:immunology; D036022:Capsid Proteins; D002417:Cattle; D000939:Epitopes / Q000276:immunology*; D005822:Genetic Vectors; D006801:Humans; D017921:Mastadenovirus / Q000145:classification / Q000235:genetics / Q000276:immunology*; D011995:Recombination, Genetic; D012703:Serotyping
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D036022:Capsid Proteins; D000939:Epitopes; C018315:hexon capsid protein, Adenovirus