[Retinoic acid inducing Fas expression in HL-60 cells].
OBJECTIVE
To investigate the effect of all-trans retinoic acid (ATRA) and 9-cis retinoic acid (9-cis RA) on the expression of Fas and the sensitivity to anti-Fas induced apoptosis in HL-60 cells.


METHODS
Fas expression was detected by flow cytometry. Anti-Fas induced apoptosis (AFIA) was determined by morphological features, DNA fragment electrophoresis, and flow cytometric cell cycle analysis.


RESULTS
Fas antigen was weakly expressed on HL-60 cells. After incubation of HL-60 cells with 10(-6) mol/L ATRA or 9-cis RA for 4 days, both the Fas expression and AFIA were significantly enhanced. 9-cis RA was more potent than ATRA in increasing the sensitivity to AFIA.


CONCLUSIONS
RA might upregulate Fas expression. Further elucidation of its molecular mechanisms might provide a rationale for new therapeutic strategies of malignant diseases.
Department of Hematology, Beijing Tongren Hospital, Capital University of Medical Sciences, Beijing.
H
H
Zeng
X
X
Zhao
Y
Y
Ma
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis; D005434:Flow Cytometry; D018922:HL-60 Cells / Q000378:metabolism; D006801:Humans; D014212:Tretinoin / Q000494:pharmacology*; D019014:fas Receptor / Q000096:biosynthesis*
D000970:Antineoplastic Agents; D019014:fas Receptor; D014212:Tretinoin