[Linkage analysis of vWD family by multi-PCR assay of microsatellite DNA in vWF gene].
OBJECTIVE
To set up a convenient method for analysis of the amplified fragment length polymorphism (amp-FLP) of two microsatellite DNA in vWF gene.


METHODS
Multi-PCR followed by denatured PAGE and silver stain were used to analyze the amp-FLP of two locus (A and B) on one gel. DNA samples from 112 normal people and two vWD families in the Shanghai area were assayed.


RESULTS
1. seven and 5 types of amp-FLP were identified on loci A and B, respectively. The heterozygote rates were 75% and 74%, respectively. 2. Haplotypes A2/B3 and A4/B3 were found to link with defective vWF genes in the two vWD families, respectively.


CONCLUSIONS
1. Multi-PCR is a fast and practical method to carry out family analysis of inherited diseases. 2. nt1980-1990 and nt2215-2380 of vWF gene are two ideal genetic labels in linkage study and hereditary consultation of vWD family.
Shanghai Institute of Hematology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025.
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Wang
D002648:Child; D002675:Child, Preschool; D004247:DNA / Q000235:genetics; D005260:Female; D008040:Genetic Linkage; D006801:Humans; D018895:Microsatellite Repeats; D010375:Pedigree; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D014842:von Willebrand Diseases / Q000235:genetics*; D014841:von Willebrand Factor / Q000235:genetics*
D014841:von Willebrand Factor; D004247:DNA