[Detection of T-cell receptor gamma chain gene rearrangement in midline T-cell lymphomas by PCR method].
OBJECTIVE
To investigate the clonality of midline T-cell lymphoma.


METHODS
Using polymerase chain reaction method and a panel of family specific primers for the variable regions of TCR-gamma gene, and totally 11 cases (22 samples) of midline T-cell lymphoma (midline malignant reticulosis) were studied.


RESULTS
Family specific monoclonal TCR-gamma gene rearrangements were found in 21 out of 22 samples (94.45%). There was no change of clonality during the course of disease.


CONCLUSIONS
There is monoclonal T-cell proliferation in midline T-cell lymphoma and the clonality is not transformed with the advance of the disease. This finding provides an evidence to support the hypothesis of monoclonal origin of neoplasm.
Department of Pathology, West China University of Medical Sciences, Chengdu 610041.
D
D
Chen
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W
Liu
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Y
Liu
D000328:Adult; D000368:Aged; D005260:Female; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D006801:Humans; D016399:Lymphoma, T-Cell / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009296:Nasal Cavity; D009303:Nasopharyngeal Neoplasms / Q000235:genetics; D009669:Nose Neoplasms / Q000235:genetics*; D016133:Polymerase Chain Reaction