Photodynamic effect of deuteroporphyrin IX and hematoporphyrin derivatives on single neuron.
The photodynamic effects of 6 new deuteroporphyrin IX derivatives with different amphiphilicity and lipophilicity, as well as effects of known hematoporphyrin derivatives Photofrin II and Photoheme on isolated crayfish mechanoreceptor neurons were studied. After 30 min photosensitization, neurons were irradiated with He-Ne laser (632.8 nm, 0.3 W/cm(2)), and changes in their firing frequency were recorded. Neuron firing was shown to be very sensitive to photodynamic effect of the studied deuteroporphyrin IX derivatives causing irreversible firing abolition at pikomolar concentrations while Photoheme and Photofrin II were effective in the nanomolar range. The most effective sensitizers were 4-(1-methyl-3-hydroxybutyl)- and 4-(1-methyl-2-acetyl-3-oxobutyl)-deuteroporphyrins. Extinction and amphiphilicity were shown to be the most important properties determining photodynamic efficiency of the studied photosensitizers.
Uzdensky
A B
AB
Rostov State University, Rostov-on-Don, 344090, Russia.
Dergacheva
O Y
OY
Zhavoronkova
A A
AA
Ivanov
A V
AV
Reshetnikov
A V
AV
Ponomarev
G V
GV
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D003400:Astacoidea; D016923:Cell Death; D003905:Deuteroporphyrins / Q000737:chemistry / Q000494:pharmacology*; D017323:Dihematoporphyrin Ether / Q000737:chemistry / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006415:Hematoporphyrins / Q000737:chemistry / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D009924:Organ Culture Techniques; D017319:Photosensitizing Agents / Q000737:chemistry / Q000494:pharmacology*
D003905:Deuteroporphyrins; D006415:Hematoporphyrins; D017319:Photosensitizing Agents; C028839:deuteroporphyrin-IX; D017323:Dihematoporphyrin Ether