Proteomics. Concepts and perspectives.
Within the last five years the field of proteomics has changed the understanding of molecular biology. Proteins manifest physiological as well as pathophysiological processes in a cell or an organism, and proteomics describes the complete protein inventory in dependence on in vivo parameters. Disease mechanism or drug effects both affect a protein profile and, vice versa, characterising protein profiles reveals information for the understanding of disease and therapy. Analytical methods for proteomics are based on conventional tools for protein characterisation. The technical challenge is the complete coverage of physico-chemical properties for thousands of proteins. Nucleic acids display a relative chemical homogeneity and therefore genomics was considered more promising in the past than proteomics. Further improvements in proteomics technologies will likely change this course with proteomics complementing genomics as a tool to study life sciences.
Preclinical R&D, Merck KGaA, Darmstadt, Germany. roland.kellner@merck.de
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D000818:Animals; D019295:Computational Biology; D019992:Databases as Topic; D015180:Electrophoresis, Gel, Two-Dimensional; D023281:Genomics; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D020543:Proteome / Q000737:chemistry / Q000235:genetics / Q000502:physiology
D020543:Proteome