MHC-I-restricted presentation of HIV-1 virion antigens without viral replication.
Dendritic cells and macrophages can process extracellular antigens for presentation by MHC-I molecules. This exogenous pathway may have a crucial role in the activation of CD8+ cytotoxic T lymphocytes during human viral infections. We show here that HIV-1 epitopes derived from incoming virions are presented through the exogenous MHC-I pathway in primary human dendritic cells, and to a lower extent in macrophages, leading to cytotoxic T-lymphocyte activation in the absence of viral protein synthesis. Exogenous antigen presentation required adequate virus-receptor interactions and fusion of viral and cellular membranes. These results provide new insights into how anti-HIV cytotoxic T lymphocytes can be activated and have implications for anti-HIV vaccine design.
Laboratoire d'Immunopathologie Viralem, Institut Pasteur, Paris, France.
F
F
Buseyne
S
S
Le Gall
C
C
Boccaccio
J P
JP
Abastado
J D
JD
Lifson
L O
LO
Arthur
Y
Y
Rivière
J M
JM
Heard
O
O
Schwartz
D002460:Cell Line; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology; D015488:HIV Antigens / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology* / Q000502:physiology; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D006801:Humans; D014771:Virion / Q000276:immunology*; D014779:Virus Replication
D000939:Epitopes; D015488:HIV Antigens; D015395:Histocompatibility Antigens Class I