Expression of aldose reductase and sorbitol dehydrogenase genes in Schwann cells isolated from rat: effects of high glucose and osmotic stress.
To investigate the polyol pathway activity in Schwann cells, we determined the mRNA levels of aldose reductase (AR) and sorbitol dehydrogenase (SDH) in cultured cells under hyperglycemic or hyperosmotic conditions using competitive RT-PCR technique. The expressions of AR and SDH mRNAs in Schwann cells were unaltered by high (30 mM) glucose content in the medium. On the other hand, osmotic stress elicited significant increases in AR mRNA without any effect on SDH mRNA expression. The levels of AR mRNA determined by this RT-PCR system were significantly correlated with AR activity, as well as the levels of sorbitol accumulated in Schwann cells cultured under hyperosmotic conditions. These findings suggest that in contrast to the induction of AR expression by osmotic stress, high glucose per se does not up-regulate expression of the enzymes constituting the polyol pathway in Schwann cells. The RT-PCR system developed in this study may be a useful tool in ascertaining the relative contributions of AR and SDH to the metabolic derangements leading to diabetic complications.
Maekawa
K
K
Division of Drug Evaluation, National Institute of Health Sciences, 1-1-43 Hoenzaka, Chuo-ku, Osaka 540-0006, Japan. maekawa@nihs.go.jp
Tanimoto
T
T
Okada
S
S
Suzuki
T
T
Suzuki
T
T
Yabe-Nishimura
C
C
D000449:Aldehyde Reductase / Q000235:genetics*; D000818:Animals; D002478:Cells, Cultured; D003929:Diabetic Neuropathies / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology*; D007064:L-Iditol 2-Dehydrogenase / Q000235:genetics*; D008297:Male; D009997:Osmotic Pressure; D011108:Polymers / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D011887:Raffinose / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012583:Schwann Cells / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*
D011108:Polymers; D012333:RNA, Messenger; C024617:polyol; D007064:L-Iditol 2-Dehydrogenase; D000449:Aldehyde Reductase; D005947:Glucose; D011887:Raffinose