Animal xenograft models for evaluation of gene transfer into human hematopoietic stem cells.
Animal xenograft models of gene therapy have become increasingly popular to study the effects of various transduction strategies on human hematopoietic stem cells (HSC). Xenograft models provide an in vivo setting in which to monitor the duration and effects of vector expression in the progeny of the transduced stem and progenitor cells. Also, the ability of HSC to home to the bone marrow and differentiate into multilineage progeny following ex vivo manipulation can only be tested in a transplantation system. The current review will discuss the murine xenograft models that have been used recently to determine optimized methods for gene transfer into normal human hematopoietic stem cells.
Division of Research Immunology/Bone Marrow Transplantation, Childrens Hospital Los Angeles, USA.
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Dao
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Tsark
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Nolta
D000818:Animals; D002453:Cell Cycle; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006085:Graft Survival; D006678:HIV / Q000235:genetics; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D008954:Models, Biological; D008979:Moloney murine leukemia virus / Q000235:genetics; D014183:Transplantation, Heterologous