Occurrence of tetrodotoxin in the skin of a rhacophoridid frog Polypedates sp. from Bangladesh.
Twenty-three specimens of a tree-frog Polypedates sp. were collected from two locations (Mymensingh and Barisal) of Bangladesh in 1999, and assayed for their toxicity scores and toxin principle. Among the tissues, only the skin of the Mymensingh specimens was found to be toxic in mouse test, with the toxicity scores of 31-923MU/g. The toxin isolated from the skin was analyzed by high-performance liquid chromatography, electrospray ionization-time of flight mass spectrometry and proton nuclear magnetic resonance, and characterized as tetrodotoxin, a toxin principle.
School of Science and Technology, Nagasaki University, 1-14, Bunkyo-machi, 852-8521, Nagasaki, Japan.
M B
MB
Tanu
Y
Y
Mahmud
K
K
Tsuruda
O
O
Arakawa
T
T
Noguchi
D000818:Animals; D001459:Bangladesh; D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D011898:Ranidae / Q000502:physiology*; D012867:Skin / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013779:Tetrodotoxin / Q000032:analysis* / Q000302:isolation & purification / Q000633:toxicity
D013779:Tetrodotoxin