Mode of antiviral activity of water soluble components isolated from Elfvingia applanata on vesicular stomatitis virus.
A preparation of water soluble components (EA) was made from carpophores of Elfvingia applanata (Pers.) Karst and its in vitro antiviral activity on vesicular stomatitis virus [(Indiana serotype, VSV(IND)] was investigated by plaque reduction assay. EA exhibited potent antiviral activity on VSV(IND) growth and negligible cytotoxicity on Vero cells, 50% effective concentration (EC50) of 104 microg/ml and 50% cytotoxic concentration (CC50) of 3,793 microg/ml, respectively. Selectivity index (SI, CC50/EC50) of EA on Vero cell and VSV(IND) was about 36.5. EA did not display either a direct virucidal effect on VSV(IND) or induction of antiviral substance by Vero cells upon its treatment. Thus, the mode of antiviral activity of EA was studied at steps of viral adsorption onto cell. When both EA and virus were added to cell monolayers, titer of cell-free virus in culture supernatant increased in ca. 30-40% compared with that of control group and titer of cell-associated virus was 60-100% higher than that of control group. These results suggested that antiviral activity of EA on VSV(IND) might be due to the hindrance of viral entry to cells at either endocytosis or loss of envelope.
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College of Pharmacy, College of Natural Sciences, Chungbuk National University, Cheongju, Korea.
Kim
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Oh
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Lee
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Lee
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Han
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D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002522:Chlorocebus aethiops; D004705:Endocytosis / Q000187:drug effects* / Q000502:physiology; D016898:Interferon-alpha / Q000494:pharmacology; D011124:Polyporaceae / Q000737:chemistry; D012995:Solubility; D014709:Vero Cells; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects* / Q000502:physiology; D014759:Viral Envelope Proteins / Q000187:drug effects* / Q000502:physiology
D000998:Antiviral Agents; D016898:Interferon-alpha; D014759:Viral Envelope Proteins