First isolation of Sarcocystis neurona from the South American opossum, Didelphis albiventris, from Brazil.
Sarcocystis neurona was isolated from sporocysts from two of eight South American opossums, Didelphis albiventris, from Brazil. Interferon gamma gene knock out (KO) mice fed sporocysts from two opossums developed neurologic sarcocystosis. S. neurona was demonstrated in the brains of infected KO mice by immunohistochemical staining with anti-S. neurona antibody. The parasite was cultivated in cell culture and S. neurona DNA was isolated from cultured merozoites. This is the first report of isolation of S. neurona from Brazil and the first report from its new host, D. albiventris.
United States Department of Agriculture, Agricultural Research Service, Animal and Natural Resources Institute, Beltsville Agricultural Research Center, Building 1040, MD 20705-2350, USA. jdubey@anri.barc.usda.gov
J P
JP
Dubey
D S
DS
Lindsay
C E
CE
Kerber
N
N
Kasai
H F
HF
Pena
S M
SM
Gennari
O C
OC
Kwok
S K
SK
Shen
B M
BM
Rosenthal
D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis; D001921:Brain / Q000469:parasitology; D001938:Brazil; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D007371:Interferon-gamma / Q000502:physiology*; D007422:Intestines / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D009893:Opossums / Q000469:parasitology*; D010274:Parasitology / Q000379:methods; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012522:Sarcocystis / Q000302:isolation & purification*
D000913:Antibodies, Protozoan; D016054:DNA, Protozoan; D007371:Interferon-gamma