Evaluation of two commercial systems and a new identification scheme based on solid substrates for identifying coagulase-negative staphylococci from bovine mastitis.
Coagulase-negative staphylococci (CNS), mainly isolated from bovine mastitis (n = 89, representing 11 different species), were used to evaluate two commercial identification systems: ID 32 Staph and Staph-Zym. The level of agreement between the ID 32 Staph and Staph-Zym systems and conventional methods was 77 and 94%, respectively. An alternative method, based on solid biochemical substrates, is also presented. This can be used for identifying novobiocin-sensitive CNS strains from bovine mastitis.
Swedish University of Agricultural Sciences, Department of Food Hygiene, Box 7009, S-75007 Uppsala, Sweden. Britt-Marie.Thorberg@lmhyg.slu.se
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Thorberg
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Brändström
D000818:Animals; D002417:Cattle; D003030:Coagulase; D005260:Female; D008403:Mass Screening / Q000592:standards / Q000662:veterinary*; D008414:Mastitis, Bovine / Q000175:diagnosis* / Q000382:microbiology; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D013210:Staphylococcus / Q000145:classification* / Q000302:isolation & purification*
D003030:Coagulase