Magnetic coupling between water and creatine protons in human brain and skeletal muscle, as measured using inversion transfer (1)H-MRS.
Using the inversion transfer technique, the possible magnetic coupling between water protons and the protons of low-molecular weight metabolites was investigated in human brain and skeletal muscle at 1.5 T. The localized (1)H-MR spectra were recorded at different times after selective inversion of the water resonance. Water inversion led to a significant transient reduction in the signal intensity of the methyl protons of creatine/phosphocreatine, in both tissues. This is indicative of magnetic coupling between the protons of water and those of creatine/phosphocreatine. Neither the choline and N-acetylaspartate protons in brain nor the protons of the trimethylammonium pool in skeletal muscle showed a significant magnetic coupling to mobile water.
Department of Experimental in vivo NMR, Image Sciences Institute, University Medical Center Utrecht, Bolognalaan 50, NL-3584, CJ Utrecht, The Netherlands.
M J
MJ
Kruiskamp
R A
RA
de Graaf
J
J
van der Grond
R
R
Lamerichs
K
K
Nicolay
D001923:Brain Chemistry; D003401:Creatine / Q000737:chemistry*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D018482:Muscle, Skeletal / Q000737:chemistry*; D010725:Phosphocreatine / Q000737:chemistry; D011522:Protons; D014867:Water / Q000737:chemistry*
D011522:Protons; D010725:Phosphocreatine; D014867:Water; D003401:Creatine