EDTA-dependent platelet phagocytosis. A cytochemical, ultrastructural, and functional characterization.
Platelet satellitosis of polymorphonuclear cells is a phenomenon induced or enhanced by the anticoagulant EDTA. In contrast with previously reported studies, the subject in the present case did not demonstrate platelet satellitism but was profoundly pseudothrombocytopenic owing to platelet phagocytosis. Virtually all polymorphonuclear leukocytes and monocytes contained numerous ingested platelets in contrast with previous cases in which phagocytosis was observed only rarely and involved ingestion of single cells. The phenomenon was documented by immunocytochemical staining and transmission electron microscopy. Autoantibodies were detected in EDTA-anticoagulated blood. However, neither platelet antibody nor phagocytosis was present when heparin, acid-citrate dextrose, or citrate was used as an alternative anticoagulant. The antibody was not temperature dependent. Mixing studies showed the transfer of the phagocytosis phenomenon to healthy donors. Although platelet function assays are typically normal in EDTA-dependent platelet satellitism, this subject showed no secondary aggregation wave in response to adenosine diphosphate and depressed adenosine triphosphate release with collagen, adenosine diphosphate, and arachidonic acid.
Drug Safety and Evaluation, Pfizer Global Research & Development, 2800 Plymouth Rd, Ann Arbor, MI 48105, USA.
K A
KA
Criswell
M A
MA
Breider
M R
MR
Bleavins
D000328:Adult; D000925:Anticoagulants / Q000494:pharmacology*; D016477:Artifacts; D001323:Autoantibodies / Q000097:blood; D001792:Blood Platelets / Q000276:immunology* / Q000648:ultrastructure; D004492:Edetic Acid / Q000494:pharmacology*; D005434:Flow Cytometry; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D009000:Monocytes / Q000276:immunology; D009504:Neutrophils / Q000276:immunology / Q000648:ultrastructure; D010587:Phagocytosis; D010974:Platelet Aggregation; D013696:Temperature; D013921:Thrombocytopenia / Q000209:etiology*
D000925:Anticoagulants; D001323:Autoantibodies; D004492:Edetic Acid