Matrix metalloproteinases expression in HL-60 promyelocytic leukemia cells during apoptosis.
Human promyelocytic leukemia HL-60 cells have been used as a model to study both the expression of matrix-metalloproteinases and the mechanisms of programmed cell death. In the present study we examined the expression of these proteases in HL-60 cells stimulated by different apoptotic triggers. As shown by zymography, HL-60 cells released three major isofroms of the matrix-degrading proteases; when the leukemic cells were grown in serum-free conditions, as well as after hyperthermia and methotrexate treatment, we found a significant loss of the constitutive production of the 92 kDa matrixmetalloprotease, with an unequivocable molecular and ultrastructural evidence of programmed cell death. These results suggest that in HL-60 cells the expression/release of matrix metalloproteases can be down-regulated in the presence of the apoptotic-induced alterations, and that the decreased matrix-degrading capacity of this leukemic cell line during apoptosis may reduce its invasive potential.
Istituto di Istologia ed Analisi di Laboratorio, Facoltà di Scienze MFN, Università degli Studi, Via E. Zeppi, 61029 Urbino, Italy.
G
G
Gazzanelli
F
F
Luchetti
S
S
Burattini
F
F
Mannello
E
E
Falcieri
S
S
Papa
D017209:Apoptosis / Q000502:physiology*; D048429:Cell Size; D016895:Culture Media, Serum-Free; D053938:DNA Fragmentation; D018922:HL-60 Cells; D006801:Humans; D020782:Matrix Metalloproteinases / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors
D016895:Culture Media, Serum-Free; D020782:Matrix Metalloproteinases