Expression of p22-phox and gp91-phox, essential components of NADPH oxidase, increases after myocardial infarction.
Recent studies have shown that oxidative stress plays an important role in cardiovascular diseases. NADPH oxidase is one of the major sources of superoxide anions and a candidate for the initiation and development of atherosclerosis, which involves the remodeling of vasculature. However, the relevance of NADPH oxidase in ventricular remodeling has not been well-characterized. This is the first report showing that the expression of p22-phox and gp91-phox, essential components of NADPH oxidase, are increased in the infarcted sites after myocardial infarction. The levels of thiobarbituric acid reactive substance, which indicates the lipid peroxidation level, and nuclear factor-kappaB (NF-kappaB) DNA binding activity are also increased in infarcted sites. Our results suggest that the increased expression of NADPH oxidase may have an effect on left ventricular remodeling by increasing the redox-sensitive NF-kappaB DNA binding activity as well as the lipid peroxidation level.
Fukui
T
T
Department of Pharmacology, Kagawa Medical University, Kagawa, Japan.
Yoshiyama
M
M
Hanatani
A
A
Omura
T
T
Yoshikawa
J
J
Abe
Y
Y
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000096:biosynthesis / Q000235:genetics; D004247:DNA / Q000378:metabolism; D006352:Heart Ventricles / Q000378:metabolism; D015227:Lipid Peroxidation; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000235:genetics*; D026901:Membrane Transport Proteins; D009203:Myocardial Infarction / Q000378:metabolism*; D009252:NADPH Dehydrogenase / Q000096:biosynthesis / Q000235:genetics*; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D010750:Phosphoproteins / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D015533:Transcriptional Activation
D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D016328:NF-kappa B; D010750:Phosphoproteins; D012333:RNA, Messenger; D009320:Atrial Natriuretic Factor; D004247:DNA; C056558:CYBB protein, human; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases; C086986:CYBA protein, human; D009252:NADPH Dehydrogenase