Crystallization and preliminary X-ray analysis of the glycerol-3-phosphate 1-acyltransferase from squash (Cucurbita moschata).
Glycerol-3-phosphate 1-acyltransferase (E.C. 2.3.1.15; G3PAT) catalyses the incorporation of an acyl group from either acyl-acyl carrier proteins (acylACPs) or acylCoAs into the sn-1 position of glycerol 3-phosphate to yield 1-acylglycerol 3-phosphate. Crystals of squash G3PAT have been obtained by the hanging-drop method of vapour diffusion using PEG 4000 as the precipitant. These crystals are most likely to belong to space group P2(1)2(1)2(1), with approximate unit-cell parameters a = 61.1, b = 65.1, c = 103.3 A, alpha = beta = gamma = 90 degrees and a monomer in the asymmetric unit. X-ray diffraction data to 1.9 A resolution have been collected in-house using a MAR 345 imaging-plate system.
Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, The University of Sheffield, Western Bank, Sheffield S10 2TN, England.
A P
AP
Turnbull
J B
JB
Rafferty
S E
SE
Sedelnikova
A R
AR
Slabas
T P
TP
Schierer
J T
JT
Kroon
I
I
Nishida
N
N
Murata
J W
JW
Simon
D W
DW
Rice
D000217:Acyltransferases / Q000737:chemistry; D003460:Crystallization; D018360:Crystallography, X-Ray; D019662:Cucurbitaceae / Q000201:enzymology*; D005992:Glycerol-3-Phosphate O-Acyltransferase / Q000737:chemistry*; D011487:Protein Conformation
D000217:Acyltransferases; D005992:Glycerol-3-Phosphate O-Acyltransferase