[Detection of T-cell receptor gamma chain gene rearrangement in midline T-cell lymphomas by PCR method].
OBJECTIVE
To investigate the clonality of midline T-cell lymphoma.


METHODS
Using polymerase chain reaction method and a panel of family specific primers for the variable regions of TCR-gamma gene, and totally 11 cases (22 samples) of midline T-cell lymphoma (midline malignant reticulosis) were studied.


RESULTS
Family specific monoclonal TCR-gamma gene rearrangements were found in 21 out of 22 samples (94.45%). There was no change of clonality during the course of disease.


CONCLUSIONS
There is monoclonal T-cell proliferation in midline T-cell lymphoma and the clonality is not transformed with the advance of the disease. This finding provides an evidence to support the hypothesis of monoclonal origin of neoplasm.
Chen
D
D
Department of Pathology, West China University of Medical Sciences, Chengdu 610041.
Liu
W
W
Liu
Y
Y
D000328:Adult; D000368:Aged; D005260:Female; D015334:Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor; D006801:Humans; D016399:Lymphoma, T-Cell / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009296:Nasal Cavity; D009303:Nasopharyngeal Neoplasms / Q000235:genetics; D009669:Nose Neoplasms / Q000235:genetics*; D016133:Polymerase Chain Reaction