Direct determination of lead in biological samples by electrothermal vaporization inductively coupled plasma mass spectrometry (ETV-ICP-MS) after furnace-fusion in the sample cuvette-tungsten boat furnace.
The newly conceived electrothermal vaporization (ETV) system using a tungsten boat furnace (TBF) sample cuvette was designed for the direct analysis of solid samples with detection by inductively coupled plasma mass spectrometry (ICP-MS). Into this small sample cuvette, a solid mixture of the biological samples and diammonium hydrogenphosphate powder as a fusion flux was placed and situated on a TBF. Tetramethylammonium hydroxide solution was added to the mixture. After the on-furnace digestion had been completed, the analyte in the cuvette was vaporized and introduced into the ICP mass spectrometer. The solid samples were analyzed by using a calibration curve prepared from the aqueous standard solutions. The detection limit was estimated to be 5.1 pg of lead, which corresponds to 10.2 ng g(-1) of lead in solid samples when a prepared sample amount of 1.0 mg was applied. The relative standard deviation for 8 replicate measurements obtained with 100 pg of lead was calculated to be 6.5%. The analytical results for various biological samples are described.
Department of Chemistry, Graduate School of Science, Hiroshima University, Higashihiroshima, Japan. Yasuaki.Okamoto@shi.hiroshima-u.ac.jp
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Okamoto
D000818:Animals; D002138:Calibration; D002417:Cattle; D004867:Equipment Design; D005506:Food Contamination / Q000032:analysis*; D005638:Fruit; D007854:Lead / Q000032:analysis*; D008099:Liver; D008974:Mollusca; D012275:Oryza; D018515:Plant Leaves; D000644:Quaternary Ammonium Compounds / Q000592:standards; D012015:Reference Standards; D012680:Sensitivity and Specificity; D012996:Solutions; D013054:Spectrophotometry, Atomic / Q000379:methods; D013696:Temperature
D000644:Quaternary Ammonium Compounds; D012996:Solutions; D007854:Lead; C027917:tetramethylammonium