[Analysis of the primary structure of hantavirus RNA, detected in organs of patients that died from HFKS in the Republic of Bashkortostan].
Fragments of the minor S and middle M segments of Hantavirus genome RNA were isolated by reverse transcription-polymerase chain reaction (RT-PCR) from the organs of patients who died from hemorrhagic fever with the renal syndrome (HFRS) during an epidemic outbreak of this infection in the Bashkortostan Republic in 1997-1998. The sizes of the resultant PCR fragments were 317 p. n. for S segment and 416 p. n. for M segment. Their primary structures (GENBANK registration numbers AJ133581 and AJ133582) were determined and they were compared with the nucleotide sequences of the respective genome sites of Hantavirus strain CG1820/Ufa-83 isolated from Clethrionomys in Bashkortostan in 1983. The homologies of nucleotide sequences of the corresponding sites of S and M segments of RNA isolate of 1997 and CG1820/Ufa-83 were 87.7 and 96.6%, respectively. The authors discuss the probability of circulation of hantaviruses differing by individual features of primary structure of genome RNA in Bashkortostan.
Institute of Biochemistry and Genetics, Ufa Research Center, Russian Academy of Sciences, Ufa.
Iu M
IuM
Nikonorov
S S
SS
Khasanova
O V
OV
Filatova
G V
GV
Ben'kovskaia
A V
AV
Chemeris
V A
VA
Vakhitov
E A
EA
Tkachenko
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002522:Chlorocebus aethiops; D004279:DNA, Viral; D006476:Orthohantavirus / Q000235:genetics*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000737:chemistry* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012426:Russia; D014709:Vero Cells
D004279:DNA, Viral; D012367:RNA, Viral