Involvement of Ca2+ in dopamine release in striatal rat slices by PF9601N and L-deprenyl.
Rat striatal slices were incubated 1 hour in Krebs buffer with carbogen by continuous perfusion. When both MAO-B inhibitors PF9601N and l-deprenyl were added in the incubation medium, at 200 microM concentration, a diminution in the dopamine content was observed. The decrease in dopamine content was partially calcium dependent and showed a different mechanism between both compounds. When 1 mM concentration EGTA was added at the Krebs incubation medium without calcium, dopamine content was partially recovered, being not affected in case of l-deprenyl. When 50 microM dantrolene (a calcium vesicular release inhibitor) was added in the Krebs incubation medium without calcium, dopamine was partially recovered for l-deprenyl, being not affected in case of PF9601N. These data indicate that dopamine release is extracellular calcium dependent in case of PF9601N, whereas l-deprenyl depends on calcium intracellular origin.
Departament de Bioquímica i de Biologia Molecular, Facultat de Medicina, Universitat Autònoma de Barcelona, 08193 Bellalterra, Spain.
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D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism*; D007211:Indoles / Q000494:pharmacology; D008744:Methylamines / Q000494:pharmacology; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012642:Selegiline / Q000494:pharmacology*
D007211:Indoles; D008744:Methylamines; D008996:Monoamine Oxidase Inhibitors; C470996:N-(2-propynyl)-2-(5-benzyloxy-indolyl) methylamine; D012642:Selegiline; D002118:Calcium; D004298:Dopamine