Simple method for clinical determination of 13 carotenoids in human plasma using an isocratic high-performance liquid chromatographic method.
We report a reversed-phase high-performance liquid chromatography method which resolves 13 identified carotenoids and nine unknown carotenoids from human plasma. A Nucleosil C18 column and a Vydac C18 column in series are used with an isocratic solvent system of acetonitrile-methanol containing 50 mM acetate ammonium-dichloromethane-water (70:15:10:5, v/v/v/v) as mobile phase at a flow-rate of 2 ml/min. The intra-day (4.5-8.3%) and inter-day (1.3-12.7%) coefficients of variation are suitable for routine clinical determinations.
Unité des Maladies Métaboliques et Micronutriments, INRA-CRNH Centre de Clermont-Ferrand/Theix, St-Genes-Champanelle, France.
B
B
Lyan
V
V
Azaïs-Braesco
N
N
Cardinault
V
V
Tyssandier
P
P
Borel
M C
MC
Alexandre-Gouabau
P
P
Grolier
D002138:Calibration; D002338:Carotenoids / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results
D002338:Carotenoids