Physical and genetic characterization of the genome of Magnetospirillum magnetotacticum, strain MS-1.
Pulsed-field gel analysis of Magnetospirillum magnetotacticum, strain MS-1, indicates that the genome is a single, circular structure of about 4.3 mb. A few genes, identified by sequence similarity, have been localized and arranged in a map with dnaA, indicating the presumed origin of replication. There are at least two rRNA operons. In addition, rRNA genes are found on a 40 kb, possibly extrachromosomal, structure. The genes thought to be involved in magnetite synthesis, bfr and magA, are located in the same 17% of the genome. A one base pair-overlap seen in the bfr genes of MS-1 is found also in the closely related magnetic strain AMB-1, but not in the non-magnetic relative A. itersonii.
Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA. lebert@cco.caltech.edu
L E
LE
Bertani
J
J
Weko
K V
KV
Phillips
R F
RF
Gray
J L
JL
Kirschvink
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics; D027682:Cation Transport Proteins; D003573:Cytochrome b Group / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004270:DNA, Circular / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005293:Ferritins / Q000235:genetics; D023061:Gene Order; D016680:Genome, Bacterial; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D021122:Protein Subunits; D015183:Restriction Mapping; D012244:Rhodospirillaceae / Q000235:genetics*; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D002352:Carrier Proteins; D027682:Cation Transport Proteins; D003573:Cytochrome b Group; D004269:DNA, Bacterial; D004270:DNA, Circular; C097085:MagA protein, Magnetospirillum; D008565:Membrane Proteins; D021122:Protein Subunits; D005293:Ferritins; C018032:bacterioferritin