Purification and characterization of the main allergen of Plantago lanceolata pollen, Pla l 1.
English plantain (Plantago lanceolata) pollen is an important cause of pollinosis in the temperate regions of North America, Australia and Europe. However, very little is known about its allergen composition. The aim of this study was to identify plantain allergens, and to isolate and characterize a major allergen. Allergens were identified by immunoblotting with individual allergic patients' sera. Isolation of the major allergen was achieved by sequential reverse-phase and size-exclusion HPLC. Allergenic characterization was performed by ELISA and immunoblotting after SDS-PAGE with sera from plantain-allergic patients. N-terminal amino acid sequence was established by Edman degradation. Allergograms showed that 13 out of the 14 sera assayed had IgE to a group of proteins with a molecular weight in the range of 16-20 kd, that turned out to be different isoforms or variants of the major allergen Pla l l. Eighteen amino acid residues from the N-terminal end of one of the isoforms, and 10 of three others, were sequenced, and a partial sequence identity with Ole e 1 was found. Prevalence of specific IgE to purified Pla l 1 in plantain allergic patients was 86%, and represents about 80% of the total IgE-binding capacity of the plantain extract. The most relevant allergen from P.lanceolata pollen, Pla l 1, has been purified and characterized. This contributes to a greater knowledge of the allergen composition of this important weed, and clears the way for the standardization of plantain allergen products in terms of major allergen content.
Research and Development Department, ALK-Abelló, C/Miguel Fleta 19, E-28037 Madrid, Spain.
B
B
Calabozo
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D
Barber
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F
Polo
D000485:Allergens / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000276:immunology; D008969:Molecular Sequence Data; D010943:Plantago / Q000276:immunology*; D010946:Plants, Medicinal; D011058:Pollen / Q000737:chemistry / Q000276:immunology*; D020033:Protein Isoforms / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology; D017386:Sequence Homology, Amino Acid
D000485:Allergens; D020033:Protein Isoforms; D007073:Immunoglobulin E