Compartmentalization of Peyer's patch anlagen before lymphocyte entry.
We have shown that Peyer's patch (PP) first develops as a simple and even cell aggregation during embryogenesis. To investigate when and how such a simple cell aggregation forms the complex PP architecture, we analyzed the distribution of cells expressing IL-7R alpha (PP inducer cells), VCAM-1 (mesenchymal cells), CD11c (dendritic cells), and mature lymphocytes by whole-mount immunostaining of 17.5 days post coitus to 2 days postpartum mouse gut. Our results show that compartmentalization of PP anlagen commences at day 18.5 of gestation by clustering and subsequent follicle formation of IL-7R alpha(+), VCAM-1(+), and CD11c(+) cells. This process adds the primitive architecture of PP anlage with several follicles in which IL-7R alpha(+) cells localize in the center, while VCAM-1(+) and CD11c(+) cells localize at the fringe. This follicle formation is accompanied by the establishment of PP-specific vascular network expressing mucosal addressin cellular adhesion molecule-1. Mature B and T lymphocytes entering in the PP anlage are distributed promptly to their own target zones; B cells to the follicle and T cells to nonfollicular zones. Our analysis of scid/scid mouse indicate that the initial processes including formation of PP-specific vascular network occur in the absence of lymphocytes. These observations indicate that the basic architecture of PP is formed by a set of cell lineages assembled during the initial phase of induction of PP anlagen before entry of mature lymphocytes.
Hashi
H
H
Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto, Japan. has@kuhp.kyoto-u.ac.jp
Yoshida
H
H
Honda
K
K
Fraser
S
S
Kubo
H
H
Awane
M
M
Takabayashi
A
A
Nakano
H
H
Yamaoka
Y
Y
Nishikawa
S
S
D000818:Animals; D000831:Animals, Newborn / Q000276:immunology; D015815:Cell Adhesion Molecules; D002449:Cell Aggregation / Q000276:immunology; D002454:Cell Differentiation / Q000276:immunology; D002465:Cell Movement / Q000276:immunology*; D004729:Endothelium, Lymphatic / Q000098:blood supply / Q000196:embryology / Q000276:immunology / Q000378:metabolism; D005260:Female; D007136:Immunoglobulins / Q000096:biosynthesis; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis; D017493:Leukocyte Common Antigens / Q000096:biosynthesis; D016131:Lymphocyte Subsets / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016513:Mice, SCID; D009088:Mucoproteins / Q000096:biosynthesis; D010581:Peyer's Patches / Q000098:blood supply / Q000166:cytology* / Q000196:embryology* / Q000276:immunology; D019707:Receptors, Chemokine / Q000096:biosynthesis; D020395:Receptors, Interleukin-7 / Q000096:biosynthesis; D016201:Receptors, Lymphocyte Homing / Q000096:biosynthesis; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis; D014699:Venules
D015815:Cell Adhesion Molecules; D007136:Immunoglobulins; C489082:Madcam1 protein, mouse; D009088:Mucoproteins; D019707:Receptors, Chemokine; D020395:Receptors, Interleukin-7; D016201:Receptors, Lymphocyte Homing; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D017493:Leukocyte Common Antigens