Immunocytochemical and electrophysiological characterization of GABA receptors in the frog and turtle retina.
The expression of GABA receptors (GABARs) was studied in frog and turtle retinae. Using immunocytochemical methods, GABA(A)Rs and GABA(C)Rs were preferentially localized to the inner plexiform layer (IPL). Label in the IPL was punctate indicating a synaptic clustering of GABARs. Distinct, but weaker label was also present in the outer plexiform layer. GABA(A)R and GABA(C)R mediated effects were studied by recording electroretinograms (ERGs) and by the application of specific antagonists. Bicuculline, the GABA(A)R antagonist, produced a significant increase of the ERG. Picrotoxin, when co-applied with saturating doses of bicuculline, caused a further increase of the ERG due to blocking of GABA(C)Rs. The putative GABA(C)R antagonist Imidazole-4-acidic acid (I4AA) failed to antagonize GABA(C)R mediated inhibition and, in contrast, appeared rather as an agonist of GABARs.
Department of Physiology, Medical University, Sofia, Bulgaria.
L
L
Vitanova
P
P
Kupenova
S
S
Haverkamp
E
E
Popova
L
L
Mitova
H
H
Wässle
D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004594:Electrophysiology; D004596:Electroretinography / Q000187:drug effects; D018755:GABA Agonists / Q000494:pharmacology; D018756:GABA Antagonists / Q000494:pharmacology; D007093:Imidazoles / Q000494:pharmacology; D007150:Immunohistochemistry; D010852:Picrotoxin / Q000494:pharmacology; D011895:Rana ridibunda / Q000502:physiology*; D018079:Receptors, GABA / Q000502:physiology*; D012160:Retina / Q000502:physiology*; D014426:Turtles / Q000502:physiology*
D001640:Bicuculline; D018755:GABA Agonists; D018756:GABA Antagonists; D007093:Imidazoles; D010852:Picrotoxin; D018079:Receptors, GABA; C005954:imidazoleacetic acid