The Plasmodium falciparum knob-associated PfEMP3 antigen is also expressed at pre-erythrocytic stages and induces antibodies which inhibit sporozoite invasion.
The expression of the pfemp3 gene and the corresponding PfEMP3 knob-associated protein in the pre-erythrocytic stages of Plasmodium falciparum was demonstrated by RT-PCR, Western blots, IFAT and IEM. The antigen was found on the surface of the sporozoite and in the cytoplasm of mature hepatic stage parasites. Immunological cross-reactivity was observed with sporozoites from the rodent malaria parasites Plasmodium yoelii yoelii and Plasmodium berghei and was exploited to assess a potential role of this protein at the pre-erythrocytic stages. Specific antibodies from immune individuals were found to inhibit P. yoelii yoelii and P. berghei sporozoite invasion of primary hepatocyte cultures. PfEMP3 should now be added to the small list of proteins expressed at the pre-erythrocytic stages of P. falciparum, and its vaccine potential now deserves to be investigated.
Unité de Parasitologie Biomédicale, Institut Pasteur, 25 and 28 Rue du Dr. Roux, Paris Cedex 15, 75724, France.
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AC
Grüner
K
K
Brahimi
W
W
Eling
R
R
Konings
J
J
Meis
M
M
Aikawa
P
P
Daubersies
C
C
Guérin-Marchand
S
S
Mellouk
G
G
Snounou
P
P
Druilhe
D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology* / Q000378:metabolism* / Q000648:ultrastructure; D015153:Blotting, Western; D003001:Cloning, Molecular; D017124:Conserved Sequence; D003429:Cross Reactions / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D004912:Erythrocytes / Q000469:parasitology; D019084:Fluorescent Antibody Technique, Indirect; D018507:Gene Expression Regulation, Developmental; D022781:Hepatocytes / Q000469:parasitology; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D008288:Malaria / Q000276:immunology / Q000469:parasitology; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism* / Q000648:ultrastructure; D016253:Microscopy, Immunoelectron; D010961:Plasmodium / Q000276:immunology; D010963:Plasmodium falciparum / Q000235:genetics / Q000254:growth & development* / Q000276:immunology* / Q000378:metabolism; D015800:Protozoan Proteins; D016053:RNA, Protozoan / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; C411566:EMP3 protein, Plasmodium falciparum; D000939:Epitopes; D007106:Immune Sera; D008565:Membrane Proteins; D015800:Protozoan Proteins; D016053:RNA, Protozoan; D011994:Recombinant Proteins