Determination of paracetamol (acetaminophen) in different body fluids and organ samples after solid-phase extraction using HPLC and an immunological method.
A solid-phase extraction method routinely used for serum samples was improved and applied to the qualitative and quantitative determination of paracetamol in different body fluids, e.g. blood, urine, cerebrospinal fluid, synovial fluid, vitreous humor, and in tissue samples. A very simple method showed best results: Body fluids were mixed with phenacetine as internal standard and phosphate buffer (pH 6.8). Then protein was precipitated using acetonitrile. After strong centrifugation the supematant was transferred to a preconditioned Bakerbond C18-SPE-column. Elution with methanol without a prior washing step showed best recovery rates. The extracts were investigated using high-performance liquid chromatography with ultraviolet detection, a photometrical and an immunochemical method.
Katedra i Zaklad Medycyny Sadowej, Akademia Medyczna im. Ludwika Rydygiera, Bydgoszcz, Poland.
E
E
Pufal
M
M
Sykutera
G
G
Rochholz
H W
HW
Schütz
K
K
Sliwka
H J
HJ
Kaatsch
D000082:Acetaminophen / Q000032:analysis* / Q000097:blood / Q000652:urine; D001826:Body Fluids / Q000737:chemistry; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007202:Indicators and Reagents; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D011041:Poisoning / Q000175:diagnosis; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013582:Synovial Fluid / Q000737:chemistry; D014822:Vitreous Body / Q000737:chemistry
D007202:Indicators and Reagents; D000082:Acetaminophen