[The suppressing effect of TIMP-3 gene transfection on the invasion and metastasis of human lung carcinoma cells line].
OBJECTIVE
The purpose of this study is to determine if increasing levels of tissue inhibitor of metalloproteinase 3 (TIMP-3) expression could suppress the malignant phenotype of human cancer cells.


METHODS
The recombinant expression vector, which contains full length cDNA of human TIMP-3, was constructed and transfected into BE1 cell line by lipofectin technique. The invasive and spontaneous metastatic potential was examined.


RESULTS
TIMP-3 mRNA expression in TIMP-3 gene transfected BE1 cells was upregulated as detected by Northern blot. The invasion of TIMP-3 gene-transfected cells across matrigel-coated filters was significantly decreased when compared with controls. Following subcutaneous injection into nude mice, the TIMP-3 transfected cells suppressed primary tumor growth, as characterized by reduced tumor incidence (9/12 vs 6/6), longer latency and reduced metastatic potential to the lungs (1/12 vs 5/6) and lymph nodes (5/12 vs 6/6).


CONCLUSIONS
The results suggest that upregulation of TIMP-3 expression in BE1 cells resulted in suppression of the invasive potential of BE1 cells in vitro as well as tumorigenic and metastatic potential in nude mice.
Niu
G
G
Department of Pathology, Beijing Medical University, Beijing 100083.
Fang
W
W
Zheng
J
J
D000818:Animals; D018286:Carcinoma, Giant Cell / Q000556:secondary*; D005786:Gene Expression Regulation; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D019717:Tissue Inhibitor of Metalloproteinase-3 / Q000096:biosynthesis / Q000235:genetics*; D014162:Transfection
D012333:RNA, Messenger; D019717:Tissue Inhibitor of Metalloproteinase-3