[The role of human papillomavirus in cyto-histological practice: distribution and prevalenceof hig-risk strains (16, 18, 31, 33, and 35) in intraepithelial lesions and neoplasia of the uterine cervix].
BACKGROUND
Many studies have already shown the association of persistent infection of human high risk papillomavirus (HPV) with the development of pre-invasive and invasive cervical disease.


METHODS
We evaluated the use of high risk HPV testing in a study of about 1908 women, aged 29-78, who attending, from 1996 to 1998, the Sant'Anna Hospital in Turin for routine, second level smears and histopathological diagnosis. We considered all cervical lesions: ASCUS, LSIL, HSIL, squamous and adeno invasive cancers. HPV testing was performed by polymerase chain reaction (PCR) using L1 consensus primers which can detect almost all infections (high and low risk types). The most important high risk HPV types (16, 18, 31, 33 and 35) were tested using specific primers.


RESULTS
The prevalence of high risk HPV was: ASCUS 42.2%, LSIL 39%, HSIL 73.5%, squamous invasive cancers 98.3% and adeno 100%. In addition HPV 16 is the most represented type in all lesions: ASCUS 40%, LSIL 62%, HSIL 71.2% squamous invasive cancers 73.3% and adeno 50.6%. In addition we study the mean age of cervical cancer onset compared with the different high risk HPV types. We found that HPV 18 related cancer occurs in younger women (mean age 41 years; range 39-42).


CONCLUSIONS
The addition of high risk HPV testing to cytology may improve early identification of women at risk for cervical cancer.
Servizio di Anatomia ed Istologia Patologica e Citodiagnostica, Laboratorio di Patologia Molecolare, Ospedale Sant'Anna, Torino.
G
G
Voglino
F
F
Poso
S
S
Privitera
F
F
Parisio
B
B
Ghiringhello
G
G
Gordini
G
G
Chiara
M
M
Massobrio
L
L
Fessia
D000230:Adenocarcinoma / Q000453:epidemiology / Q000821:virology*; D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000453:epidemiology / Q000821:virology*; D016384:Consensus Sequence; D017931:DNA Primers; D015344:DNA Probes, HPV; D004273:DNA, Neoplasm / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D007558:Italy / Q000453:epidemiology; D008875:Middle Aged; D027383:Papillomaviridae / Q000145:classification / Q000302:isolation & purification / Q000472:pathogenicity*; D030361:Papillomavirus Infections / Q000453:epidemiology / Q000821:virology*; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012306:Risk; D012703:Serotyping; D013045:Species Specificity; D013048:Specimen Handling; D014412:Tumor Virus Infections / Q000453:epidemiology / Q000821:virology*; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology / Q000821:virology*; D016662:Virus Integration; D002578:Uterine Cervical Dysplasia / Q000453:epidemiology / Q000821:virology*
D017931:DNA Primers; D015344:DNA Probes, HPV; D004273:DNA, Neoplasm; D004279:DNA, Viral