Multidirectional approach to study peritoneal dialysis fluid biocompatibility in a chronic peritoneal dialysis model in the rat.
BACKGROUND
Peritoneal dialysis causes the functional and morphological changes in the peritoneum that result from the bioincompatibility of dialysis solutions. We present a model of chronic peritoneal dialysis in the rat that can be used for testing the biocompatibility of dialysis fluids. Methods and Results. Long-term exposure of the peritoneum to dialysis solutions can be performed in rats with implanted peritoneal catheters. Sampling of the dialysate allows the evaluation of intraperitoneal inflammation by examining cell differential and dialysate cytokine levels. Peritoneal permeability can be evaluated at designed time intervals with the peritoneal equilibration test (PET). At the end of dialysis, peritoneal histology is studied with light and electron microscopy.


CONCLUSIONS
Such a multidirectional approach is an effective way to test biocompatibility of dialysis solutions.
Wieczorowska-Tobis
K
K
Department of Pathophysiology, University of Medical Sciences, Poznań, Poland.
Polubinska
A
A
Wisniewska
J
J
Pawlaczyk
K
K
Kuzlan-Pawlaczyk
M
M
Filas
V
V
Breborowicz
A
A
Oreopoulos
D G
DG
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D002408:Catheters, Indwelling; D015314:Dialysis Solutions / Q000009:adverse effects / Q000494:pharmacology*; D008297:Male; D008854:Microscopy, Electron; D010530:Peritoneal Dialysis; D010537:Peritoneum / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D010538:Peritonitis / Q000139:chemically induced / Q000473:pathology; D010539:Permeability; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D001672:Biocompatible Materials; D015314:Dialysis Solutions