Interleukin 1beta, interleukin-6, and tumor necrosis factor-alpha in endometriotic tissue and in endometrium.
OBJECTIVE
To compare the levels of IL-1beta, IL-6, and TNFalpha in endometriotic tissue and in endometrium from women with endometriosis and healthy controls.


METHODS
Open.


METHODS
Department of Obstetrics and Gynecology at a university hospital.


METHODS
Twenty-six women with endometriosis and 22 controls operated on for clinical indications.


METHODS
ELISA in homogenized tissue samples collected during surgery.


METHODS
Levels of IL-1beta, IL-6, and TNFalpha in tissue homogenates.


RESULTS
The three types of tissue differed significantly with respect to all three cytokines. Endometriotic tissue had significantly higher concentrations of IL-1beta than endometrium from both patients with endometriosis and healthy controls. Both endometriotic tissue and endometrium from patients had significantly higher concentrations of IL-6, and endometriotic tissue had significantly lower concentration of TNFalpha than did endometrium from controls. IL-1beta showed a cycle phase dependence that was significant in endometrium from patients, being higher in the secretory than in the follicular phase. IL-1beta was significantly higher in endometrioma than in lesions of other localizations. Concentrations of IL-1beta and IL-6 were positively correlated in endometriotic tissue and in endometrium from controls. No other significant correlations were found.


CONCLUSIONS
This study has shown a significant production of IL-1beta, IL-6, and TNFalpha in endometriotic tissue and endometrium, with significant differences between the tissue types, indicating a deviating cytokine pattern in both endometriotic tissue and endometrium from women with endometriosis compared with that from healthy controls.
Department of Obstetrics and Gynecology, Huddinge University Hospital, and Karolinska Institutet, Stockhom, Sweden. agneta.bergqvist@obgyn.hs.sll.se
A
A
Bergqvist
C
C
Bruse
M
M
Carlberg
K
K
Carlström
D004715:Endometriosis / Q000378:metabolism*; D004717:Endometrium / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis*; D015850:Interleukin-6 / Q000032:analysis*; D008597:Menstrual Cycle; D010049:Ovarian Diseases / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*
D007375:Interleukin-1; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha