Induction of peroxiredoxins in transplanted livers and demonstration of their in vitro cytoprotection activity.
Peroxiredoxin (Prx)-I and -II belong to a new class of antioxidants. Here, we report that they are induced by ischemia/reperfusion (I/R) in transplanted livers. Hypothesizing that Prxs are induced to protect liver from oxidative damage, we transduced these human genes into murine NIH-3T3 cells. The overexpressed Prxs made the cells more resistant to t-butylhydroperoxide-induced apoptosis. These results indicate that Prx-I and Prx-II are induced by the transplantation process and can protect cells against oxidant damage in tissue culture. Thus, proper genetic manipulations of Prxs may be useful in increasing the success rate of organ transplantation.
Division of Surgical Oncology, UCLA School of Medicine, Los Angeles, CA 90095-1782, USA. hshau@ucla.edu
H
H
Shau
A
A
Merino
L
L
Chen
C C
CC
Shih
S D
SD
Colquhoun
D016475:3T3 Cells; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D017209:Apoptosis; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000378:metabolism; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins; D006801:Humans; D015151:Immunoblotting; D008099:Liver / Q000378:metabolism; D016031:Liver Transplantation; D008164:Luminescent Proteins / Q000378:metabolism; D051379:Mice; D018384:Oxidative Stress; D010544:Peroxidases / Q000235:genetics / Q000378:metabolism*; D054464:Peroxiredoxins; D010957:Plasmids / Q000378:metabolism; D015427:Reperfusion Injury; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014161:Transduction, Genetic
D000975:Antioxidants; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins; D010544:Peroxidases; D054464:Peroxiredoxins