Nitric oxide-related experiments on peritoneal solute transport in the rabbit.
BACKGROUND
It is unclear whether nitric oxide (NO) is important in regulating peritoneal transport during non-infected peritoneal dialysis.


METHODS
In 13 rabbits, 250 mg/l L-arginine, a substrate for NO synthesis, was added to a 3.86% glucose dialysis solution. N:(G)-monomethyl-L-arginine (L-NMMA) 25 mg/1, an inhibitor of NO synthase, was added to the dialysate in 10 rabbits. Standard peritoneal permeability analyses in rabbits were used to analyse the effects of these interventions on solute transport during 1-h dwells. The addition of 4.5 mg/l nitroprusside to the dialysate in five rabbits was used for validation of this model.


RESULTS
Nitroprusside caused an 86% (48-233%) increase in albumin clearance, which is similar to the nitroprusside-induced increase found in humans (70%). Contrary to human studies, no effect was found on the mass transfer area coefficient (MTAC) of urea and creatinine, or on glucose absorption. L-Arginine did not affect either the MTAC of urea and creatinine, or the absorption of glucose. Peritoneal albumin clearance increased 18% (-24 to 609%). This resembles the NO-mediated effects of nitroprusside. Addition of L-NMMA caused no change in the solute transport rate.


CONCLUSIONS
The rabbit dialysis model can be used for analysing the effects of interventions on peritoneal permeability characteristics, although the rabbit peritoneal membrane is probably less sensitive to NO compared with that of humans. L-Arginine-induced effects are similar to those of nitroprusside, which suggests that these effects possibly are mediated by NO. As L-NMMA did not affect peritoneal transport, it is unlikely that NO is involved in the regulation of peritoneal permeability in rabbits.
Department of Nephrology, Academic Medical Center, University of Amsterdam. Dianet Foundation, Utrecht, The Netherlands.
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DG
Struijk
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Douma
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Krediet
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Zweers
D000818:Animals; D001120:Arginine / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects / Q000502:physiology; D007274:Injections, Intraperitoneal; D009569:Nitric Oxide / Q000502:physiology*; D009599:Nitroprusside / Q000494:pharmacology; D010537:Peritoneum / Q000378:metabolism*; D011817:Rabbits; D012709:Serum Albumin / Q000378:metabolism
D012709:Serum Albumin; D009599:Nitroprusside; D009569:Nitric Oxide; D001120:Arginine