Changes in dendritic cell subsets in the lymph nodes of rhesus macaques after application of glucocorticoids.
Corticosteroids are used therapeutically as potent immunosuppressive and antiinflammatory drugs for a broad spectrum of diseases. Although corticosteroids are known to inhibit the production of many cytokines in activated T cells, there is also evidence for increases in IL-4 and in some cases IFNgamma production. These conflicting results may be caused by contrary effects of corticosteroids on different cell types involved in immune regulation, for instance antigen presenting cells (APC) versus T cells. In the present study we simultaneously investigated the effect of local as well as systemic application of glucocorticoids (GCC) on the phenotype of APC in the skin as well as the lymph nodes in a model primate species, the rhesus macaque. Using a range of APC markers, including CD68, HAM56, HLA-DR, CD1a, p55, RFD-1, and costimulatory molecules CD40, CD80, and CD86 we document the close phenotypic resemblance of rhesus and human APC. We noted that topical GCC treatment specifically lead to a marked decrease in the number of CD1a expressing cells in the draining lymph nodes. However, the number of CD1a positive cells in peripheral lymph nodes was not affected by systemic GCC treatment. Importantly, by performing double staining of CD1a with RFD-1 we observed a shift in the expression pattern of these dendritic cell markers in the lymph nodes, with an increase in the number of RFD-1 single positive cells relative to CD1a single positive and CD1a/RFD-1 double positive cells. These findings suggest that GCC treatment results in the presence of phenotypically more mature APC.
Department of Virology, Biomedical Primate Research Centre, Rijswijk, The Netherlands. koopman@bprc.nl
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G
Koopman
A G
AG
Dalgleish
B S
BS
Bhogal
A G
AG
Haaksma
J L
JL
Heeney
D000287:Administration, Topical; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000276:immunology* / Q000494:pharmacology; D000938:Antigen-Presenting Cells / Q000145:classification / Q000276:immunology; D015703:Antigens, CD / Q000032:analysis; D001132:Arm; D002990:Clobetasol / Q000031:analogs & derivatives / Q000276:immunology* / Q000494:pharmacology; D003713:Dendritic Cells / Q000145:classification / Q000166:cytology / Q000276:immunology*; D003907:Dexamethasone / Q000276:immunology* / Q000494:pharmacology; D005938:Glucocorticoids / Q000276:immunology* / Q000494:pharmacology; D008198:Lymph Nodes / Q000166:cytology / Q000187:drug effects; D008214:Lymphocytes / Q000145:classification / Q000276:immunology; D008253:Macaca mulatta; D008297:Male; D013848:Thigh
D000893:Anti-Inflammatory Agents; D015703:Antigens, CD; D005938:Glucocorticoids; D003907:Dexamethasone; D002990:Clobetasol