Sperm protein 17 is a novel cancer-testis antigen in multiple myeloma.
Various studies have demonstrated the aberrant expression of normal testicular proteins in neoplastic cells. These proteins collectively form the new class of tumor antigens called cancer-testis (CT) antigens. Their selective normal tissue expression makes them ideal antigens for immune targeting of the malignant disease. In this study, the expression of a spermatozoa protein, Sp17, in multiple myeloma was investigated. It was found that Sp17 is detectable in tumor cells from 12 of 47 (26%) myeloma patients. Reverse transcription polymerase chain reaction (RT-PCR) and Western blot analysis detected Sp17 transcripts and proteins, respectively. Northern blot analysis and RT-PCR demonstrated that Sp17 transcripts were detected only in normal testis, supporting its tissue specificity. Since a high proportion of normal individuals develop antibodies against Sp17 following vasectomy, Sp17 is likely to be a highly immunogenic protein in vivo. Sp17 is therefore a novel member of the CT antigen family and should be an ideal target for immunotherapy of multiple myeloma.
Center for Immunology and Microbial Disease and the Department of Medicine, Albany Medical College, Albany, NY 12208, USA. seah.lim@mail.amc.edu
S H
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Lim
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Wang
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Chiriva-Internati
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Y
Xue
D000951:Antigens, Neoplasm / Q000378:metabolism*; D000954:Antigens, Surface; D001324:Autoantigens / Q000378:metabolism; D001853:Bone Marrow / Q000378:metabolism / Q000473:pathology; D002148:Calmodulin-Binding Proteins; D002352:Carrier Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008565:Membrane Proteins; D009101:Multiple Myeloma / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D013737:Testis / Q000737:chemistry; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D001324:Autoantigens; D002148:Calmodulin-Binding Proteins; D002352:Carrier Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger; C090172:SPA17 protein, human