Activity and mRNA abundance of Delta-5 and Delta-6 fatty acid desaturases in two human cell lines.
We analyzed fatty acid biosynthesis in Chang and ZR-75-1 cells. Both cell lines could desaturate and further elongate substrates for Delta-5 desaturase. ZR-75-1 but not Chang cells showed Delta-6 desaturation of 18:2n-6, 18:3n-3, 24:4n-6 and 24:5n-3. In both cell lines, the mRNA abundance can be related to Delta-5 or Delta-6 fatty acid desaturase activities. These results suggest that desaturase genes could have, at least in part, independent control mechanisms and that Delta-6 desaturase impairment is not specific to any particular step of the fatty acid metabolic pathways, which may diminish the rationale for the existence of at least two distinct enzymes.
de Antueno
R J
RJ
QuantaNova Canada Ltd., P.O. Box 818, B4N 4H8, Kentville, N.S., Canada. r.deantueno@mailexcite.com
Allen
S J
SJ
Ponton
A
A
Winther
M D
MD
D001943:Breast Neoplasms / Q000201:enzymology*; D002458:Cell Fractionation; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D000090943:Delta-5 Fatty Acid Desaturase; D044943:Fatty Acid Desaturases / Q000235:genetics / Q000378:metabolism*; D015525:Fatty Acids, Omega-3 / Q000378:metabolism; D043371:Fatty Acids, Omega-6; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D050602:Linoleoyl-CoA Desaturase; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D012333:RNA, Messenger / Q000378:metabolism*
D000090943:Delta-5 Fatty Acid Desaturase; D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6; D005231:Fatty Acids, Unsaturated; D012333:RNA, Messenger; D044943:Fatty Acid Desaturases; D050602:Linoleoyl-CoA Desaturase