[Low-molecular-weight glycoprotein from cattle blood serum: structure and properties].
Amino acid composition, structure, and physicochemical properties of a low-molecular-weight glycoprotein from cattle blood serum (SGP) were studied. The content of carbohydrates (represented by mannose-rich oligosaccharides) amounted to 45-50 wt %. The value of specific partial heat of SGP, measured by differential scanning calorimetry (DSC), equaled 1.8 J/g.K, which is characteristic of unfolded proteins. Circular dichroic (CD) spectra of SGP led us to conclude that it is not highly structured and that it occurs in the shape of a statistical globule. The protein was deglycated using anhydrous trifluoromethane sulfonate (TFMS), after which its amino acid composition and the sequence of a fragment were determined. The results indicate that SGP is a protein not studied previously.
Iamskov
I A
IA
Nesmeyanov Institute of Organoelemental Compounds of the Russian Academy of Sciences, Moscow, 117813 Russia.
Vinogradov
A A
AA
Danilenko
A N
AN
Maslova
L A
LA
Rybakova
E Iu
EIu
Iamskova
V P
VP
D000818:Animals; D001798:Blood Proteins / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification; D002152:Calorimetry, Differential Scanning; D002417:Cattle; D006023:Glycoproteins / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D011487:Protein Conformation; D017421:Sequence Analysis
D001798:Blood Proteins; D006023:Glycoproteins