Fluoride uptake in human teeth from fluoride-releasing restorative material in vivo and in vitro: two-dimensional mapping by EPMA-WDX.
Class V cavities were prepared in the upper and lower left second premolars from an individual under infiltration anesthesia. The cavities were filled with fluoride- releasing resin (TF). One month later, the teeth were extracted. As a control (in vitro), the upper and lower right second premolars were extracted at the time of the cavity preparation in vivo. Immediately after extraction, class V cavities were prepared and filled with TF, and immersed in normal saline solution for 1 month at 37 degrees C. All four premolars were bisected longitudinally and the fluoride uptake around the cavity wall on the cut surface was measured using an electron probe microanalyzer-wavelength dispersive X-ray method. The fluoride uptake was given in the form of a two-dimensional map. Comparison of the observed values of each corresponding part of the tooth in vivo and in vitro revealed no characteristic differences. The maps were quite analogous as a whole.
Department of Restorative Dentistry and Endodontology, Division of Oral Infections and Disease Control, Osaka University Graduate School of Dentistry, Osaka, Japan. yhiroko@dent.osaka.u.ac.jp
H
H
Yamamoto
Y
Y
Iwami
T
T
Unezaki
Y
Y
Tomii
S
S
Ebisu
D000328:Adult; D001641:Bicuspid / Q000378:metabolism* / Q000473:pathology; D002327:Cariostatic Agents / Q000737:chemistry / Q000493:pharmacokinetics*; D003737:Dental Cavity Preparation / Q000145:classification; D003764:Dental Materials / Q000737:chemistry*; D003793:Dental Restoration, Permanent; D004577:Electron Probe Microanalysis; D005260:Female; D005459:Fluorides / Q000737:chemistry / Q000493:pharmacokinetics*; D005500:Follow-Up Studies; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008855:Microscopy, Electron, Scanning; D018709:Statistics, Nonparametric
D002327:Cariostatic Agents; D003764:Dental Materials; D005459:Fluorides