A rapid and highly sensitive method for diagnosis of equine influenza by antigen detection using immuno-PCR.
A rapid and highly sensitive method for diagnosis of influenza by detecting viral antigens using immuno-PCR has been developed. The sensitivity of the immuno-PCR for the detection of the nonstructural protein 1 (NS 1) antigenically common among influenza A viruses was 10(1.0-2.0) times higher than that of RT-PCR for the detection of the viral genome. For the detection of the hemagglutinin (HA) subtype-specific antigen, this assay using anti-HA monoclonal antibodies attained a sensitivity of up to 10(7.0-8.0) times higher than those by virus isolation or by RT-PCR.
Department of Disease Control, Hokkaido University Graduate School of Veterinary Medicine, Sapporo 060-0818, Japan.
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Ozaki
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Sugita
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Kida
D000818:Animals; D002478:Cells, Cultured; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006734:Horse Diseases / Q000175:diagnosis* / Q000821:virology; D006736:Horses; D009980:Influenza A virus / Q000235:genetics; D009976:Orthomyxoviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012680:Sensitivity and Specificity