The role of Helicobacter pylori virulence factors in interleukin production by monocytic cells.
Helicobacter pylori infection results in chronic gastritis, which is initiated by the release of cytokines like interleukin (IL)-12 and IL-8 from mononuclear cells, and IL-8 from gastric epithelial cells. The severity of gastritis is influenced both by host factors and by bacterial factors such as the Cag proteins and the vacuolating cytotoxin VacA. Amounts of IL-12 and IL-8 produced by monocytic THP-1 cells differed considerably between the eight H. pylori isolates tested, but in contrast to H. pylori-induced IL-8 production by gastric epithelial cells, did not correlate to the Cag and VacA types of the strains. Apparently, in addition to Cag and VacA, other bacterial factors determine the extent in which H. pylori induced IL production in monocytes.
de Jonge
R
R
Department of Gastroenterology, Academic Hospital, Vrije Universiteit, Amsterdam, The Netherlands.
Kusters
J G
JG
Timmer
M S
MS
Gimmel
V
V
Appelmelk
B J
BJ
Bereswill
S
S
van Vliet
A H
AH
Meuwissen
S G
SG
Kist
M
M
Vandenbroucke-Grauls
C M
CM
Kuipers
E J
EJ
D000942:Antigens, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology / Q000378:metabolism / Q000502:physiology; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000378:metabolism; D005753:Gastric Mucosa / Q000166:cytology / Q000276:immunology; D006681:HLA-D Antigens; D016480:Helicobacter pylori / Q000235:genetics / Q000276:immunology*; D006801:Humans; D018928:Immunity, Mucosal; D018664:Interleukin-12 / Q000032:analysis / Q000096:biosynthesis*; D016209:Interleukin-8 / Q000032:analysis / Q000096:biosynthesis*; D009000:Monocytes / Q000276:immunology*; D014774:Virulence / Q000235:genetics
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D006681:HLA-D Antigens; D016209:Interleukin-8; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori; D018664:Interleukin-12