Leukocyte-specific gene 1 protein (LSP1) is involved in chemokine KC-activated cytoskeletal reorganization in murine neutrophils in vitro.
Leukocyte-specific gene 1 protein (LSP1) is a cytoskeletal-associated protein of leukocytes that in vitro cross-links F-actin into extensively branched bundles of mixed polarity. In this study, we examined chemotaxis and superoxide production in neutrophils prepared from wild-type (WT) and Lsp1 knockout mice. Compared to WT neutrophils, Lsp1-/- neutrophils showed impairment in both migration speed and chemotaxis direction during chemokine KC-directed chemotaxis. When examined by confocal microscopy, chemotaxing Lsp1-/- neutrophils showed abnormal morphologies. They had discontinuous primary actin-rich cortexes and large membrane protrusions. When stimulated by phorbol 12-myristate 13-acetate (PMA), Lsp1-/- peritoneal neutrophils produce more superoxide than WT. The data presented suggest that LSP1 plays important roles in the regulation of neutrophil morphology, motility, and superoxide production.
Hannigan
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Department of Pathology, University of Connecticut Health Center, Farmington 06030-3105, USA. michaelhanniga@snet.net
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D000199:Actins / Q000378:metabolism; D000818:Animals; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology; D054360:Chemokine CXCL1; D018925:Chemokines; D019743:Chemokines, CXC; D002634:Chemotaxis, Leukocyte / Q000235:genetics / Q000502:physiology; D016207:Cytokines / Q000502:physiology*; D003595:Cytoplasmic Streaming / Q000502:physiology; D003599:Cytoskeleton / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D008840:Microfilament Proteins; D018613:Microscopy, Confocal; D009504:Neutrophils / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D013481:Superoxides / Q000378:metabolism
D000199:Actins; D002135:Calcium-Binding Proteins; D054360:Chemokine CXCL1; D018925:Chemokines; D019743:Chemokines, CXC; C475895:Cxcl1 protein, mouse; D016207:Cytokines; C057701:Lsp1 protein, mouse; D008840:Microfilament Proteins; D013481:Superoxides; C074302:keratinocyte-derived chemokines