Restricted growth of ent(-) and tonB mutants of Salmonella enterica serovar Typhi in human Mono Mac 6 monocytic cells.
Monocytes and macrophages are an important host defense in humans infected with Salmonella enterica serovar Typhi. Bacterial ability to survive in these cells is therefore a crucial virulence characteristic of this pathogen. In this study, we demonstrate that growth of a Salmonella enterica serovar Typhi enterochelin synthesis mutant and a tonB mutant in the human monocyte cell line Mono Mac 6 is restricted compared to that of the parental wild-type Ty2 strain. These results suggest that enterochelin- and TonB-mediated iron uptake plays a role in S. enterica serovar Typhi pathogenesis, and also suggest that mutations in iron uptake may attenuate S. enterica serovar Typhi strains for human beings.
Gorbacheva
V Y
VY
Department of Microbiology and Immunology, New York Medical College, Valhalla, NY 10595-1690, USA.
Faundez
G
G
Godfrey
H P
HP
Cabello
F C
FC
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport; D002460:Cell Line; D004251:DNA Transposable Elements; D004758:Enterobactin / Q000378:metabolism*; D005798:Genes, Bacterial; D006801:Humans; D007501:Iron / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009000:Monocytes / Q000382:microbiology*; D009154:Mutation; D012485:Salmonella typhi / Q000235:genetics* / Q000254:growth & development* / Q000472:pathogenicity; D014774:Virulence
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D008565:Membrane Proteins; C057616:tonB protein, Bacteria; D004758:Enterobactin; D007501:Iron