Inhibitory effects of sanguinarine on monoamine oxidase activity in mouse brain.
The effects of benzophenanthridine alkaloids, such as sanguinarine and chelidonine, on monoamine -oxidase (MAO) activity in mouse brain were investigated. Sanguinarine showed an inhibitory effect on MAO activity in a concentration dependent manner (53.4% inhibition at 25 microM). However, chelidonine did not inhibit MAO activity. The IC(50) value of sanguinarine was 24.5 microM. Sanguinarine inhibited non-competitively MAO activity using kynuramine as a substrate. The K(i) value for sanguinarine was 22.1 microM. These results suggest that sanguinarine partially contributes to the regulation of catecholamine content.
College of Pharmacy, Chungbuk National University, San 48, Kaeshin-Dong, Heungduk-Ku, Cheongju 361-763, Republic of Korea.
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D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D053119:Benzophenanthridines; D001600:Berberine Alkaloids; D001921:Brain / Q000187:drug effects*; D002492:Central Nervous System Depressants / Q000494:pharmacology*; D007546:Isoquinolines; D007735:Kynuramine / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008995:Monoamine Oxidase / Q000378:metabolism*; D010617:Phenanthridines / Q000494:pharmacology*; D010946:Plants, Medicinal
D000470:Alkaloids; D053119:Benzophenanthridines; D001600:Berberine Alkaloids; D002492:Central Nervous System Depressants; D007546:Isoquinolines; D010617:Phenanthridines; D007735:Kynuramine; C062047:chelidonine; C005705:sanguinarine; D008995:Monoamine Oxidase