Expression of the chemokine RANTES in pulmonary Wegener's granulomatosis.
Wegener's granulomatosis (WG) is an inflammatory, destructive, angiotropic lesion. The inflammatory process involves accumulation of macrophages, lymphocytes, and polymorphonuclear neutrophils. We studied 6 lung biopsy specimens from patients with WG to characterize the cellular infiltrate and to analyze the mechanism of immune cell recruitment. We show that lymphocytes accumulating in WG lesions are mostly memory CD4(+)CD45RO(+) T lymphocytes and, although less numerous, CD8(+)CD45RO(+) T lymphocytes. Few if any B lymphocytes or natural killer cells are present within lesions. The chemokine RANTES (regulated upon activation in normal T cells, expressed and secreted) has been reported to recruit memory T lymphocytes and macrophages selectively. We used reverse-transcription polymerase chain reaction, in situ hybridization, and immunohistochemistry to study its production in WG. RANTES was expressed at a higher level in WG lungs than in normal controls, especially around microabscesses. As visualized immunohistochemically in serial sections with anti-RANTES monoclonal antibody, RANTES production was produced mainly by macrophages. Expression of the gene coding for interferon-gamma (IFN-gamma), a potent RANTES inducer, was also studied. Its expression was also much stronger in WG than in controls. Our observations are consistent with a cascade of events leading to the recruitment of immune cells in WG, sequentially involving production of IFN-gamma by T lymphocytes and RANTES production by macrophages, leading to the homing of memory T-helper lymphocytes and macrophages. HUM PATHOL 32:320-326.
INSERM U131, Institut Paris Sud sur les Cytokines, Clamart, France.
A
A
Coulomb-L'Hermine
F
F
Capron
W
W
Zou
F
F
Piard
F
F
Galateau
P
P
Laurent
M C
MC
Crevon
P
P
Galanaud
D
D
Emilie
D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000737:chemistry / Q000473:pathology; D001706:Biopsy; D015496:CD4-Positive T-Lymphocytes / Q000737:chemistry / Q000473:pathology; D018946:Chemokine CCL5 / Q000032:analysis / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D014890:Granulomatosis with Polyangiitis / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007371:Interferon-gamma / Q000235:genetics; D017493:Leukocyte Common Antigens / Q000032:analysis; D008171:Lung Diseases / Q000378:metabolism* / Q000473:pathology; D008264:Macrophages / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000737:chemistry / Q000473:pathology
D000911:Antibodies, Monoclonal; D018946:Chemokine CCL5; D007371:Interferon-gamma; D017493:Leukocyte Common Antigens