Beta-carotene antagonizes the effects of eicosapentaenoic acid on cell growth and lipid peroxidation in WiDr adenocarcinoma cells.
The effects of combinations between eicosapentaenoic acid (EPA) and beta-carotene on cell growth and lipid peroxidation were investigated in human WiDr colon adenocarcinoma cells. EPA alone was able to inhibit the growth of WiDr cells in a dose- and time-dependent manner. Such an inhibition involved fatty acid peroxidation, as shown by the remarkable increase in the levels of Malondialdehyde (MDA) in EPA-treated cells. Beta-carotene was capable of reducing the growth inhibitory effects of EPA and the levels of MDA in a dose- and a time-dependent manner. In addition, EPA increased beta-carotene consumption in WiDr cells. This study provides evidence that beta-carotene can antagonize the effects of EPA on colon cancer cell growth and lipid peroxidation.
Institute of General Pathology, Catholic University, Rome, Italy.
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Palozza
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Calviello
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Maggiano
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Lanza
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Ranelletti
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Bartoli
D000230:Adenocarcinoma; D002455:Cell Division / Q000187:drug effects* / Q000502:physiology; D003110:Colonic Neoplasms; D015118:Eicosapentaenoic Acid / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D015227:Lipid Peroxidation / Q000187:drug effects* / Q000502:physiology; D008315:Malondialdehyde / Q000032:analysis; D013997:Time Factors; D014407:Tumor Cells, Cultured; D019207:beta Carotene / Q000493:pharmacokinetics / Q000494:pharmacology*
D019207:beta Carotene; D008315:Malondialdehyde; D015118:Eicosapentaenoic Acid