Analysis of host responses of guinea pigs during Helicobacter pylori infection.
Host responses of guinea pigs infected with Helicobacter pylori were investigated. Passaged H. pylori colonised the stomach for up to 13 weeks after infection, but after 1 month the number of bacteria fell sharply. Specific antibodies, predominantly of the IgG2 subtype, were present from week 3 onwards. Antibodies to urease A and flagella were abundant. Severe inflammation of the gastric mucosa and damage to the stomach epithelium was seen. Infiltrates of mononuclear cells and eosinophils were found near the parietal glands. As infection progressed, inflammation and tissue damage became more localised and more variable between individual animals. These parameters can be used as markers for colonisation of the stomach by H. pylori.
Division of Bacteriology, National Institute for Biological Standards and Control, Potters Bar, UK. srijpkema@nibsc.ac.uk
S G
SG
Rijpkema
Z
Z
Durrani
G
G
Beavan
J R
JR
Gibson
J
J
Luck
R J
RJ
Owen
G R
GR
Auda
D000818:Animals; D004269:DNA, Bacterial / Q000032:analysis; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006168:Guinea Pigs; D016481:Helicobacter Infections / Q000145:classification / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D016480:Helicobacter pylori / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000032:analysis
D004269:DNA, Bacterial; D007074:Immunoglobulin G