[Enhancement of apoptotic sensitivity induced by UV irradiation on the p53-transducted K562 cells].
OBJECTIVE
To answer whether wild-type p53 can sensitize K562 cell apoptosis induced by UV irradiation.


METHODS
K562 cells transducted with retrovirus encoding wild-type p53 were irradiated by ultraviolet for different time and then cultured for different time. Apoptosis was detected by TDT end labelling technique, DNA fragmentation and MTT assay.


RESULTS
TDT end labelling and DNA fragmentation showed that apoptosis induced by 8 min UV-irradiation differed significantly between K562-neo and K562-p53 cells.


CONCLUSIONS
Wild-type p53 can enhance apoptosis induced by UV-irradiation on K562 cells.
Central Laboratory, People's Hospital, Beijing Medical University, Beijing 100044.
D
D
Chen
S
S
Wang
G
G
Cao
D017209:Apoptosis / Q000235:genetics / Q000528:radiation effects; D016158:Genes, p53; D006801:Humans; D020014:K562 Cells / Q000473:pathology; D012190:Retroviridae / Q000235:genetics; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000494:pharmacology*; D014466:Ultraviolet Rays
D016159:Tumor Suppressor Protein p53