The lymphoid-specific cofactor OBF-1 is essential for the expression of a V(H) promoter/HS1,2 enhancer-linked transgene in late B cell development.
Mice deficient for the lymphoid-specific cofactor OBF-1 display reduced levels of IgG, IgA and IgE. To examine whether the lowered immunoglobulin expression is linked to reduced activity of IgH cis-regulatory elements, OBF-1(-/-) mice were crossed with mice expressing transgenes driven by a V(H) or beta-globin promoter linked to the HS1,2 enhancer. Here we show that OBF-1 is essential for the induced expression of a V(H) promoter-linked transgene, in contrast to a beta-globin promoter-dependent transgene, in LPS/IL-4 or CD40-stimulated splenic B cells. Furthermore, impaired transgene expression is observed in OBF-1(-/-) peritoneal B cells. This deficiency may be linked to OBF-1, as peritoneal cells from normal mice express OBF-1 protein constitutively. Our data link OBF-1 to IgH gene expression in late B lymphoid development.
Center for Genomics Research, Karolinska Institutet, S-171 77, Stockholm, Sweden. tove.andersson@aventis.com
T
T
Andersson
A
A
Samuelsson
P
P
Matthias
S
S
Pettersson
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects; D019013:CD40 Antigens / Q000276:immunology; D002454:Cell Differentiation; D004742:Enhancer Elements, Genetic; D005260:Female; D015870:Gene Expression; D005914:Globins / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D015847:Interleukin-4 / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D013154:Spleen / Q000166:cytology; D015534:Trans-Activators / Q000378:metabolism*; D019076:Transgenes
D019013:CD40 Antigens; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D008070:Lipopolysaccharides; C091546:POU2AF1 protein, human; C490424:Pou2af1 protein, mouse; D015534:Trans-Activators; D015847:Interleukin-4; D005914:Globins