beta-Conglycinin and glycinin in high-protein soybean seeds.
The agronomic performance and storage proteins of high seed protein lines of soybeans [Glycine max L. (Merr.)] were investigated to determine if the two major storage proteins, beta-conglycinin and glycinin, contribute to the increased protein content of high seed protein lines. Subunits of these two major storage proteins were estimated by scanning SDS-PAGE gels by scanning densitometry. The relative rankings of the lines with respect to seed size and protein content were not different between years in one environment over 5 years, but oil and total protein and oil contents and the ratio of protein to oil differed. The alpha', alpha, and beta subunits of beta-conglycinin were significantly higher in the high-protein lines except CX797-115, CX804-108, CX804-3, D81-8498, and NC-2-62. The acidic A(3) polypeptide of glycinin was significantly higher in high-protein lines except 76-48773, CX804-108, CX804-3, D81-8498, and NC-2-62, whereas the acidic polypeptides A(1,2,4) of glycinin were significantly higher in all of the high-protein lines. The basic polypeptides of glycinin were significantly higher in all high seed protein lines except D81-8259. In conclusion, high-protein lines appear to contain more beta-conglycinin and glycinin than normal-protein soybean lines, and the amounts of subunits and polypeptides differ among lines.
Soybean and Alfalfa Research Laboratory, Building 006, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705, USA.
R W
RW
Yaklich
D052179:Antigens, Plant; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005916:Globulins / Q000032:analysis*; D010940:Plant Proteins / Q000032:analysis; D021122:Protein Subunits; D055314:Seed Storage Proteins; D012639:Seeds / Q000737:chemistry*; D013024:Soybean Oil / Q000737:chemistry; D030262:Soybean Proteins; D013025:Glycine max / Q000737:chemistry*; D013045:Species Specificity
D052179:Antigens, Plant; D005916:Globulins; D010940:Plant Proteins; D021122:Protein Subunits; D055314:Seed Storage Proteins; D030262:Soybean Proteins; C013894:beta-conglycinin protein, Glycine max; D013024:Soybean Oil; C011474:glycinin