Genetic and physical mapping of two centromere-proximal regions of chromosome IV in Aspergillus nidulans.
Chromosome IV is the smallest chromosome of Aspergillus nidulans. The centromere-proximal portion of the chromosome was mapped physically using overlapping clones of a cosmid genomic library. Two contiguous segments of a physical map, based on restriction mapping of cosmid clones, were generated, together covering more than 0.4 Mb DNA. A reverse genetic mapping approach was used to establish a correlation between physical and genetic maps; i.e., marker genes were integrated into physically mapped segments and subsequently mapped by mitotic and meiotic recombination. The resulting data, together with additional classical genetic mapping, lead to a substantial revision of the genetic map of the chromosome, including the position of the centromere. Comparison of physical and genetic maps indicates that meiotic recombination is low in subcentromeric DNA, its frequency being reduced from 1 crossover per 0.8 Mb to approximately 1 crossover per 5 Mb per meiosis. The portion of the chromosome containing the functional centromere was not mapped because repeat-rich regions hindered further chromosome walking. The size of the missing segment was estimated to be between 70 and 400 kb.
Center for Process Biotechnology, Technical University of Denmark, Lyngby, 2800, Denmark. aal@ibt.dtu.dk
A
A
Aleksenko
M L
ML
Nielsen
A J
AJ
Clutterbuck
D001233:Aspergillus nidulans / Q000235:genetics*; D002503:Centromere / Q000235:genetics*; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D020451:Contig Mapping; D003360:Cosmids / Q000235:genetics; D003434:Crossing Over, Genetic; D008040:Genetic Linkage; D005838:Genotype; D008540:Meiosis / Q000235:genetics; D011995:Recombination, Genetic; D015183:Restriction Mapping