A new method for radioiodination of polysaccharides and its use in biodistribution studies of an immunomodulating glycoconjugate (Immunoferon).
A new method for the labeling of polysaccharides by using tyrosine derivatives after CNBr activation is presented, along with its use for pharmacokinetic studies of an orally active immunomodulating noncovalent glycoconjugate, which was administered labeled either in the polysaccharide or the protein moiety. The relationship between the obtained results and the mechanism of action are also discussed.
Research and Development Department, Industrial Farmacéutica Cantabria S.A., Madrid, Spain.
A
A
Guerrero
A
A
Brieva
J P
JP
Pivel
D000276:Adjuvants, Immunologic / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D000818:Animals; D002130:Calcium Phosphates / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D002850:Chromatography, Gel; D005772:Gastrointestinal Transit / Q000187:drug effects; D006020:Glycopeptides / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D007457:Iodine Radioisotopes; D007553:Isotope Labeling / Q000379:methods*; D008297:Male; D051379:Mice; D011134:Polysaccharides / Q000737:chemistry; D014018:Tissue Distribution; D014443:Tyrosine
D000276:Adjuvants, Immunologic; D002130:Calcium Phosphates; D006020:Glycopeptides; D007457:Iodine Radioisotopes; D011134:Polysaccharides; D014443:Tyrosine; C038773:Immunoferon