Functional study and regional mapping of 44 hormono-regulated genes isolated from a porcine granulosa cell library.
cDNA clones from a pig granulosa cell cDNA library were isolated by (differential hybridisation for follicle stimulating hormone (FSH) regulation in granulosa cells in a previous study. The clones that did not match any known sequence were studied for their expression in granulosa cells (treated or not by FSH) and in fresh isolated ovarian follicles mainly by comparative RT-PCR analysis. These results give functional data on genes that may be implicated in follicular growing. These ESTs have been localised on the porcine genome, using a somatic cell hybrid panel, providing new type I markers on the porcine map and information on the comparative map between humans and pigs.
Laboratoire de génétique cellulaire, Institut national de la recherche agronomique, Castanet-Tolosan, France. tosser@toulouse.inra.fr
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Tosser-Klopp
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Bonnet
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Yerle
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Hatey
D000818:Animals; D015152:Blotting, Northern; D002478:Cells, Cultured; D002874:Chromosome Mapping; D020224:Expressed Sequence Tags; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D005786:Gene Expression Regulation; D015723:Gene Library; D016678:Genome; D006107:Granulosa Cells / Q000187:drug effects*; D006801:Humans; D006080:Ovarian Follicle / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012689:Sequence Homology, Nucleic Acid; D013552:Swine / Q000235:genetics*
D005640:Follicle Stimulating Hormone