Insect silk contains both a Kunitz-type and a unique Kazal-type proteinase inhibitor.
Insect silk is made up of structural fibrous (fibroins) and sticky (sericins) proteins, and contains a few small peptides of hitherto unknown functions. We demonstrate that two of these peptides inhibit bacterial and fungal proteinases (subtilisin, proteinase K and pronase). These 'silk proteinase inhibitors' 1 and 2 (SPI 1 and 2) are produced in the middle section of the silk-secreting glands prior to cocoon spinning and their production is controlled at transcription level. The full length cDNA of pre-SPI 1 contains 443 nucleotides and encodes a peptide of 76 amino-acid residues, of which 20 make up a signal sequence. The mature SPI 1 (6056.7 Da, 56 residues) is a typical thermostable Kunitz-type proteinase inhibitor with Arg in P1 position. The cDNA of pre-SPI 2 consists of 260 nucleotides and yields a putative secretory peptide of 58 amino-acid residues. The functional SPI 2 (3993 Da, 36 residues) is a single-domain Kazal-type proteinase inhibitor with unique structural features: free segment of the N-terminus is reduced to a single amino-acid residue, lack of CysI and CysV precludes formation of the A-ring and provides increased flexibility to the C-ring, and absence of several residues around the normal position of CysV shortens and changes the alpha helix segment of the protein. The structure reveals that the length and arrangement of the B-ring, including exposure of the P1 residue, and the position of the C-terminus relative to the B-loop, are essential for the activity of the Kazal-type inhibitors.
Entomological Institute, Academy of Sciences, and the Faculty of Biological Sciences, University of South Bohemia, Branisovská, Ceské Budejovice, Czech Republic.
X
X
Nirmala
D
D
Kodrík
M
M
Zurovec
F
F
Sehnal
D000595:Amino Acid Sequence; D000818:Animals; D007611:Aprotinin / Q000737:chemistry; D015152:Blotting, Northern; D002851:Chromatography, High Pressure Liquid; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D019476:Insect Proteins / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009036:Moths / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D047011:Silk; D014359:Trypsin Inhibitor, Kazal Pancreatic / Q000737:chemistry; D014361:Trypsin Inhibitors / Q000302:isolation & purification*
D018076:DNA, Complementary; D019476:Insect Proteins; D047011:Silk; D014361:Trypsin Inhibitors; C425549:silk proteinase inhibitor 1, Galleria mellonella; C425550:silk proteinase inhibitor 2, Galleria mellonella; D014359:Trypsin Inhibitor, Kazal Pancreatic; D007611:Aprotinin