Co-expression of GFAP, vimentin and cytokeratins in GL-15 glioblastoma cell line.
Glial fibrillary acidic protein (GFAP), vimentin (Vi) and cytokeratin (CK) intermediate filament (IF) proteins were studied in glioblastoma cell line GL-15. The immunofluorescence staining revealed strong positive staining for vimentin in all cultured cells. Approximately 20% of analyzed cells showed strong and 50% moderate intensity of staining for GFAP. About 3% of all cells were positively stained with a mixture of anti-CK monoclonal antibodies. The expression of all IF was not in relation to the cell density or days in vitro after passage. The double immunofluoresce revealed that all CK-positive cells express GFAP and vimentin. This study demonstrates the heterogeneity of the clonal GL-15 glioma cell line which consists in three immunocytochemically distinct cell types: Vi+/GFAP-/CK-, Vi+/GFAP+/CK-, and Vi+/GFAP+/CK+. These findings give further evidence about the expression of non-glial IF in cultured glioma cells.
Perzelcová
A
A
Department of Anatomy, School of Medicine, Comenius University, Bratislava, Slovak Republic. Perzelova@fmed.uniba.sk
Máciková
Tardy
M
M
Mráz
P
P
Steno
J
J
Bízik
I
I
D001932:Brain Neoplasms / Q000378:metabolism*; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis*; D005909:Glioblastoma / Q000378:metabolism*; D006801:Humans; D007633:Keratins / Q000096:biosynthesis*; D008856:Microscopy, Fluorescence; D014407:Tumor Cells, Cultured; D014746:Vimentin / Q000096:biosynthesis*
D005904:Glial Fibrillary Acidic Protein; D014746:Vimentin; D007633:Keratins