Intraspecific divergence of Saccharomyces kluyveri as revealed by the nucleotide sequences of 18S-28S rRNA spacer regions and alpha-galactosidase MEL genes.
In the five strains classified as the yeast Saccharomyces kluyveri, several substitutions were observed in the two internal transcribed spacer regions between 18S and 28S rRNA. A PCR reaction with primers targeted to the MEL1 gene of Saccharomyces cerevisiae amplified fragments of the expected size, and those sequences showed significant divergence in the strains of S. kluyveri.
Oda
Y
Y
Upland Agriculture Research Center, Hokkaido National Agricultural Experiment Station, Kasai, Japan. yujioda@mmr.affrc.go.jp
Fujisawa
T
T
D015139:Blotting, Southern; D004271:DNA, Fungal / Q000032:analysis / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D010802:Phylogeny; D012440:Saccharomyces / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D000519:alpha-Galactosidase / Q000235:genetics*
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D000519:alpha-Galactosidase