Predicting quantitative phytochemical markers in single Echinacea plants or clones from their DNA fingerprints.
Amplified restricted fragment length polymorphism (AFLP) data analysis was found to be a statistically significant predictor of phytochemical markers in cultivated Echinacea purpurea germplasm and some related wild species. Over 50 accessions grown under greenhouse conditions were subjected to AFLP analysis and the same assessed for content of tetraene and cichoric acid by high pressure liquid chromatography. The first and second canonical correlation of DNA variables and the phytochemical variables were significant. Individual regressions of cichoric acid and dodeca-2E, 4E, 8Z, 10E/Z-tetraenoic acid isobutyl amide predicted by DNA polymorphism analysis against actual HPLC determined values were nearly linear. Mantel's test showed that there was a weak correlation but a strong association of values of the phytochemical variables and the DNA polymorphism data.
Agriculture & Agri-Food Canada, Research Branch, Eastern Cereal and Oilseed Research Centre, Ottawa, ON. baumbr@em.agr.ca
B R
BR
Baum
S
S
Mechanda
J F
JF
Livesey
S E
SE
Binns
J T
JT
Arnason
D001095:Arachidonic Acids / Q000032:analysis*; D019659:Asteraceae / Q000737:chemistry / Q000235:genetics; D002109:Caffeic Acids; D002726:Chlorogenic Acid / Q000031:analogs & derivatives / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D016172:DNA Fingerprinting; D018744:DNA, Plant / Q000235:genetics; D020900:Echinacea / Q000737:chemistry* / Q000235:genetics*; D005819:Genetic Markers; D016018:Least-Squares Analysis; D010946:Plants, Medicinal; D011110:Polymorphism, Genetic; D012044:Regression Analysis; D013045:Species Specificity; D013386:Succinates; D013644:Tartrates / Q000032:analysis*
D001095:Arachidonic Acids; D002109:Caffeic Acids; D018744:DNA, Plant; D005819:Genetic Markers; D013386:Succinates; D013644:Tartrates; D002726:Chlorogenic Acid; C100435:chicoric acid