Human phosphoglucose isomerase: expression, purification, crystallization and preliminary crystallographic analysis.
Phosphoglucose isomerase (PGI) is the second enzyme in the glycolytic pathway and catalyzes an aldose-ketose isomerization. Outside the cell, PGI has been found to function as both a cytokine and as a growth factor. The human pgi gene was cloned and the expressed enzyme was purified to homogeneity. Isomorphous crystals were obtained under two conditions and belong to the P2(1)2(1)2(1) space group, with unit-cell parameters a = 80.37, b = 107.54, c = 270.33 A. A 94.7% complete data set was obtained and processed to a limiting resolution of 2.6 A. The asymmetric unit contains two hPGI dimers according to density calculations, a self-rotation function map and molecular-replacement solution.
Laboratory of Protein Crystallography and Structural Biology, Physics Institute of São Carlos, University of São Paulo - USP, Av. Trabalhador Sãocarlense 400, PO Box 369, 13566-590 São Carlos - SP, Brazil.
A T
AT
Cordeiro
P H
PH
Godoi
L F
LF
Delboni
G
G
Oliva
O H
OH
Thiemann
D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli; D005956:Glucose-6-Phosphate Isomerase / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008958:Models, Molecular; D009097:Multienzyme Complexes / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification
D009097:Multienzyme Complexes; D011994:Recombinant Proteins; D005956:Glucose-6-Phosphate Isomerase