The porcine gonadotropin-releasing hormone receptor gene (GNRHR): genomic organization, polymorphisms, and association with the number of corpora lutea.
The interaction of gonadotropin-releasing hormone (GNRH) and its receptor (GNRHR) is critical in the endocrine regulation of reproduction. The gene (GNRHR) encoding the receptor has been mapped to porcine chromosome 8. There is evidence for three quantitative trait loci (QTL) influencing ovulation rate on this chromosome. We obtained an almost complete sequence (3993 bp, excluding intron 1) of the porcine GNRHR gene using PCR-based comparative genomic walking and inverse genomic walking approaches. Twelve polymorphisms were detected by sequencing of pooled DNA of Chinese Taihu and European Large White pigs, including 7 base substitutions and 5 insertions-deletions (indels). A F2 population of Meishan x European Large White pigs was genotyped for a TG indel in the promoter region, and a C/G substitution in the 3' UTR (untranslated region). A significant association of the C/G substitution with number of corpora lutea at first parity was observed.
Jiang
Z
Z
Centre for Genetic Improvement of Livestock, Department of Animal and Poultry Science, University of Guelph, Canada. zjiang@lsd.uoguelph.ca
Gibson
J P
JP
Archibald
A L
AL
Haley
C S
CS
D020413:3' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D003338:Corpus Luteum / Q000166:cytology*; D003433:Crosses, Genetic; D017931:DNA Primers; D005260:Female; D016678:Genome; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D019655:Quantitative Trait, Heritable; D011966:Receptors, LHRH / Q000235:genetics*; D017384:Sequence Deletion; D013552:Swine
D020413:3' Untranslated Regions; D017931:DNA Primers; D011966:Receptors, LHRH