Impaired restriction endonuclease cleavage of DNA modified with N-methyl-N-nitrosourea.
This paper examines the ability of several restriction enzymes to cleave lambda DNA treated in vitro with N-methyl-N-nitrosourea. An impaired digestion was observed with all tested enzymes suggesting that the sequence specific restriction endonucleases are either unable to recognize carcinogen-modified sequences or to insert endonucleolytic cuts at such sites.
Zentrum der Biologischen Chemie, Universität Frankfurt, GFR.
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Boehm
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Drahovsky
D010582:Bacteriophage lambda / Q000235:genetics; D002273:Carcinogens / Q000494:pharmacology*; D018736:DNA Adducts / Q000737:chemistry; D004249:DNA Damage; D004262:DNA Restriction Enzymes / Q000494:pharmacology*; D004279:DNA, Viral / Q000187:drug effects*; D004587:Electrophoresis, Agar Gel; D008770:Methylnitrosourea / Q000494:pharmacology*
D002273:Carcinogens; D018736:DNA Adducts; D004279:DNA, Viral; D008770:Methylnitrosourea; D004262:DNA Restriction Enzymes