Investigation of human papillomavirus in transitional cell carcinomas of the urinary bladder in South Africa.
OBJECTIVE
To investigate the prevalence of human papillomavirus in transitional cell carcinoma of the urinary bladder in South Africa.


METHODS
Ninety-one archival samples of bladder transitional cell carcinoma were subjected to polymerase chain reaction (PCR) and non-isotopic in situ hybridisation (NISH) for the detection of human papillomavirus 6, 11, 16, 18, 31, and 33 genotypes.


RESULTS
HPV was detected in only one case with PCR. HPV was not detected in any of the cases subjected to the NISH system.


CONCLUSIONS
This study shows that although HPV has been shown to be associated with uterine cervical and esophageal squamous cell carcinomas in South Africa, this virus is not present in the transitional cell carcinoma of the urinary bladder in this geographical location. It is suggested that other factors, including nitrosamine exposure, p53 mutation, and additional unknown chromosomal events, may play a role in the carcinogenesis of this neoplasm in the bladder.
Department of Anatomical Pathology, University of the Witwatersrand, Johannesburg, South African Institute for Medical Research.
M
M
Sur
K
K
Cooper
U
U
Allard
D001609:Beta-Globulins / Q000032:analysis; D002295:Carcinoma, Transitional Cell / Q000737:chemistry / Q000235:genetics / Q000473:pathology / Q000821:virology*; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D030361:Papillomavirus Infections / Q000150:complications* / Q000473:pathology; D016133:Polymerase Chain Reaction; D013019:South Africa; D014412:Tumor Virus Infections / Q000150:complications* / Q000473:pathology; D001749:Urinary Bladder Neoplasms / Q000737:chemistry / Q000235:genetics / Q000473:pathology / Q000821:virology*
D001609:Beta-Globulins; D017931:DNA Primers; D004279:DNA, Viral