Aspartic protease in leaves of common bean (Phaseolus vulgaris L.) and cowpea (Vigna unguiculata L. Walp): enzymatic activity, gene expression and relation to drought susceptibility.
Four cultivars of related species, common bean and cowpea, which exhibit different degrees of drought resistance, were submitted to water stress by withholding irrigation. Drought induced an increase in endoproteolytic activity, being higher in susceptible cultivars (bean) than in tolerant ones (cowpea). An aspartic protease activity was found to be strongly induced especially in bean. From a cowpea leaf cDNA library, a full length aspartic protease precursor cDNA was obtained. Transcript accumulation in response to water stress indicated that the expression of the gene was constitutive in cowpea and transcriptionally up-regulated in bean. The results showed that drought-tolerant and drought-susceptible bean plants differ regarding aspartic protease precursor gene expression.
Cruz de Carvalho
M H
MH
Laboratoire de Biochimie et Physiologie de l'Adaptation Végétale, Université Paris 7 Denis Diderot, France.
d'Arcy-Lameta
A
A
Roy-Macauley
H
H
Gareil
M
M
El Maarouf
H
H
Pham-Thi
A T
AT
Zuily-Fodil
Y
Y
D000040:Abscisic Acid / Q000494:pharmacology; D000222:Adaptation, Physiological; D016282:Aspartic Acid Endopeptidases / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis; D018744:DNA, Plant / Q000032:analysis; D007887:Fabaceae / Q000201:enzymology* / Q000502:physiology; D015870:Gene Expression; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000201:enzymology / Q000502:physiology; D010946:Plants, Medicinal; D012333:RNA, Messenger / Q000096:biosynthesis; D019685:Rosales / Q000201:enzymology* / Q000502:physiology
D018076:DNA, Complementary; D018744:DNA, Plant; D010937:Plant Growth Regulators; D012333:RNA, Messenger; D000040:Abscisic Acid; D016282:Aspartic Acid Endopeptidases