Proteome analysis of activated murine B-lymphocytes.
Proteins extracted from murine B-lymphocytes after in vitro stimulation by lipopolysaccharide were separated by two-dimensional (2-D) polyacrylamide gel electrophoresis and analyzed by matrix assisted laser desorption/ionization mass spectrometry. Structural information on the protein entities from 153 spots was obtained. Since many of these spots occur as members of spot families, a smaller number --98 genes-- was found to be coding for the identified spots. The elucidated proteins belong to groups of functional categories; we found 26 enzymes, 36 regulatory proteins, 15 chaperones, 15 structural proteins, 4 immunoglobulins, 1 ribosomal and 1 histone protein. A comparison between expected and observed molecular masses yields a good correlation for the majority of the compared spot entities. This set of proteins now identified in the context of a lymphocyte 2-D gel pattern should advance further studies on lymphocyte functions.
Basel Institute for Immunology, Switzerland. frey@bii.ch
J R
JR
Frey
M
M
Fountoulakis
I
I
Lefkovits
D000818:Animals; D001402:B-Lymphocytes / Q000737:chemistry* / Q000187:drug effects; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005260:Female; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D008934:Mitogens / Q000494:pharmacology; D011506:Proteins / Q000032:analysis* / Q000145:classification; D020543:Proteome
D008070:Lipopolysaccharides; D008934:Mitogens; D011506:Proteins; D020543:Proteome