Successful 48-h liver preservation by controlling nutritional status of donor and recipient.
The nutritional status of the donor has been shown to affect the outcome of liver transplantation in the rat. It has been proposed that this may be due to inhibition of Kupffer cell induced injury to the reperfused organ, which leads to an inflammatory type response. In this study we investigated how altering the nutritional status of the recipient affects the outcome of liver transplantation after preservation of the liver for 44 or 48 h in the University of Wisconsin (UW) solution. The nutritional status of the rats was altered by either fasting or by feeding an essential fatty acid free diet (EFAD) for 2 months. This type of diet has been shown to reduce significantly the inflammatory response in rats. Survival after 44-h preservation of livers from fed donors (fed a standard laboratory diet) transplanted to fed recipients was 29% (2/7) but increased to 80% (4/5) when the recipient was fed the EFAD diet. After 48-h preservation, there were no survivors under either of these two dietary combinations. However, survival was 100% after 48-h preservation if the donor had been fasted for 4 days and the recipient was fed the EFAD. These results showed that the nutritional status of the donor and recipient are important factors in the outcome of liver transplantation. How nutritional factors affect liver preservation and transplantation are not clear but may be related to the inflammatory response regulated by Kupffer cells and circulating neutrophils in the liver, both of which are influenced by the diet of the animal.
Sumimoto
R
R
First Department of Surgery, Hiroshima Prefectual Hospital, Japan.
Fukuda
Y
Y
Gambiez
L
L
Oshiro
H
H
Dohi
K
K
Southard
J H
JH
Belzer
F O
FO
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D004041:Dietary Fats; D005215:Fasting; D005228:Fatty Acids, Essential; D005978:Glutathione; D006085:Graft Survival / Q000502:physiology; D007328:Insulin; D008099:Liver; D016031:Liver Transplantation / Q000379:methods* / Q000502:physiology; D009752:Nutritional Status; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D011887:Raffinose; D051381:Rats; D011914:Rats, Inbred BN; D013997:Time Factors
D004041:Dietary Fats; D005228:Fatty Acids, Essential; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose