p300-mediated acetylation of human transcriptional coactivator PC4 is inhibited by phosphorylation.
The human positive coactivator 4 (PC4) acts as a general coactivator for activator-dependent transcription, the activity of which is regulated negatively by phosphorylation. We report here that PC4 can be acetylated specifically by another coactivator, p300. Interestingly, phosphorylation of PC4 by casein kinase II inhibits the p300-mediated acetylation. Mass spectral analysis revealed that there are at least two lysine residues acetylated in PC4, as a result of which its DNA binding activity is stimulated.
Transcription and Disease Laboratory, Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur, Bangalore-560 064, India.
B R
BR
Kumar
V
V
Swaminathan
S
S
Banerjee
T K
TK
Kundu
D000107:Acetylation; D000123:Acetyltransferases / Q000378:metabolism*; D000818:Animals; D047390:Casein Kinase II; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D006367:HeLa Cells; D051548:Histone Acetyltransferases; D006657:Histones / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D017874:Immediate-Early Proteins; D008239:Lysine; D008565:Membrane Proteins; D051379:Mice; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D012097:Repressor Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D014157:Transcription Factors; D014158:Transcription, Genetic; D050880:p300-CBP Transcription Factors; D000099224:p300-CBP-Associated Factor
D000123:Acetyltransferases; D047390:Casein Kinase II; D018797:Cell Cycle Proteins; D051548:Histone Acetyltransferases; D006657:Histones; D017874:Immediate-Early Proteins; D008239:Lysine; D008565:Membrane Proteins; D017346:Protein Serine-Threonine Kinases; D011994:Recombinant Proteins; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D050880:p300-CBP Transcription Factors; D000099224:p300-CBP-Associated Factor; C490043:IFRD2 protein, human; C490042:Ifrd2 protein, mouse