Comparison of cyclin A and MIB-1 expression in astrocytic tumors using image-based cell analysis system.
Traditional prognostic indicators for astrocytic tumors include tumor size, type, and histologic grade. Data suggest that tumor growth fraction assessed by MIB-1 is an important predicator of survival. Cyclin A, like MIB-1, is a recently described specific marker of proliferation, detectable primarily in S phase of the cell cycle as it undergoes progression to G2 phase. Thirty-seven cases of astrocytic tumors--14 cases of World Health Organization grade 1 and 2 (low grade tumors), 8 cases of grade 3 (anaplastic astrocytoma), and 15 cases of grade 4 (glioblastoma multiforme)--were simultaneously evaluated using routine paraffin immunohistochemical methods with commercial antibodies against MIB-1 and cyclin A. The results were quantitated using a Cell Analysis System (CAS) 200 image analyzer. The mean percentage positive nuclear area for MIB-1 was 3.32% in grade 1 and 2, 19.27% in grade 3, and 24.00% in grade 4 astrocytic tumors. Cyclin A showed a similar pattern of positivity in the same cases: 2.84% in grade 1 and 2, 16.27% in grade 3, and 24.88% in grade 4 astrocytic tumors. The data suggest that both proliferation markers correlated significantly with histologic grade. Cyclin A appears to be as good an indicator of brain tumor proliferation as MIB-1. Because cyclin A is detectable primarily in the S phase of the cell cycle, the fraction of cells positive for cyclin A should allow for a more accurate indicator of tumor progression.
Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City 66160, USA.
Y
Y
Ren
P
P
Munyer
J J
JJ
Kepes
M
M
Cansler
J
J
Collins
B
B
Gayed
O
O
Tawfik
D034961:Antigens, Nuclear; D001254:Astrocytoma / Q000378:metabolism* / Q000473:pathology; D014408:Biomarkers, Tumor / Q000378:metabolism; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D002453:Cell Cycle; D019925:Cyclin A / Q000378:metabolism*; D005909:Glioblastoma / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D007150:Immunohistochemistry / Q000379:methods; D019394:Ki-67 Antigen; D009687:Nuclear Proteins / Q000378:metabolism*; D011379:Prognosis; D012189:Retrospective Studies
D034961:Antigens, Nuclear; D014408:Biomarkers, Tumor; D019925:Cyclin A; D019394:Ki-67 Antigen; D009687:Nuclear Proteins