Coeliac- and enteropathy-associated autoantibodies in Spanish insulin-dependent diabetes mellitus patients and their relation to HLA antigens.
The frequency of reticulin (ARA), endomysium (EmA), and gut epithelial cell (GECA) autoantibodies, and gliadin antibodies (AGA), was investigated in 86 Spanish diabetic patients by indirect immunofluorescence (IFI) and ELISA, along with their HLA phenotype. Four patients (5%) showed ARA-IgG (R1 pattern), eight (9%) showed AGA-IgG, and eight (9%) showed AGA-IgA. No EmA or GECA-positive patients were found. In diabetic patients, HLA-DR7 is increased in ARA-IgG+ vs. ARA-IgG- (though not significantly), and HLA-DR6 and HLA-DQ1 are significantly increased in the AGA-IgG+ group vs. the AGA-IgG- group. Comparison with a non-diabetic coeliac group showed that HLA-DR4 and HLA-DQ3 are significantly increased in the AGA-IgA+ group, whereas HLA-DQ2 shows a significant decrease in the AGA-IgG+ and AGA-IgA+ patients. Finally, when compared to the healthy group, HLA-DR7 frequency is decreased in the ARA-IgG- group, while HLA-DQ3 is significantly increased and HLA-DR6 and HLA-DQ1 significantly decreased in the AGA-IgG- group.Altogether, these data suggest that the genetic background leading to the appearance of coeliac-specific autoantibodies in Spanish diabetic patients differ depending on the autoantibody produced and is also different to the genetic background leading to diabetes in Spain.
Departament of Immunology, Hospital 12 de Octubre, Universidad Complutense de Madrid, 28041 Madrid, Spain.
J M
JM
Martín-Villa
J C
JC
López-Suárez
M
M
Pérez-Blas
J
J
Martínez-Laso
S
S
Ferre-López
C
C
García-Torre
G
G
Lledó
J
J
Manzanares
A
A
Arnaiz-Villena
D000293:Adolescent; D000483:Alleles; D001323:Autoantibodies / Q000097:blood*; D002446:Celiac Disease / Q000276:immunology*; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000235:genetics / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D005903:Gliadin / Q000276:immunology; D006683:HLA-DQ Antigens / Q000097:blood* / Q000235:genetics; D006684:HLA-DR Antigens / Q000097:blood* / Q000235:genetics; D006801:Humans; D007413:Intestinal Mucosa / Q000276:immunology*; D009077:Mucins / Q000276:immunology; D010641:Phenotype; D012155:Reticulin / Q000276:immunology; D052116:Sialomucins; D013030:Spain
D001323:Autoantibodies; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D009077:Mucins; D012155:Reticulin; D052116:Sialomucins; D005903:Gliadin