A new human immunodeficiency virus type 1 circulating recombinant form from Tanzania.
It is becoming increasingly important to identify and to study human immunodeficiency virus type 1 (HIV-1) circulating recombinant forms (CRFs) with evidence of epidemic spread, since mosaic strains arise frequently, especially in populations where multiple subtypes cocirculate. We describe the almost complete nucleotide sequence of 3 subtype C and D recombinant viruses, selected from a pool of 13 D(gag)-D/C/D(env) perinatally infected infants from Dar es Salaam, Tanzania. All three genomes had cross-over points with approximately the same genomic localization. The subtype C-like sequences were located within pol, vif, vpr, vpu, the first exons of rev and tat, V3, and the U3-R regions of the LTR. Phylogenetic analyses of the full-length genomic sequences from these viruses showed the formation of a distinct subcluster on the HIV-1 subtype D branch. The pattern of recombination of genomes belonging to this new CRF, named CRF10_CD, might have resulted from independent recombination events occurring at high frequency or from a single source that originated earlier in this population. Future surveys will be needed to determine the potential of this CRF for epidemic spread.
Koulinska
I N
IN
Harvard AIDS Institute and Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts 02115, USA.
Ndung'u
T
T
Mwakagile
D
D
Msamanga
G
G
Kagoma
C
C
Fawzi
W
W
Essex
M
M
Renjifo
B
B
D016384:Consensus Sequence; D016679:Genome, Viral; D015658:HIV Infections / Q000151:congenital / Q000453:epidemiology / Q000821:virology*; D015497:HIV-1 / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007223:Infant; D010802:Phylogeny; D011995:Recombination, Genetic / Q000235:genetics*; D013636:Tanzania