Analysis of amplified variable number tandem repeat loci for evaluation of engraftment after hematopoietic stem cell transplantation.
Many methods have been used to evaluate engraftment of donor cells after hematopoietic stem cell transplantation in allogeneic recipients. The identification of variable number tandem repeat loci in the human genome and the development of methods for testing amplified DNA segments containing these polymorphic loci have provided a novel and highly convenient approach for assessing the success of hematopoietic stem cell transplantation. We discuss the advantages and limitations of this approach as it has been implemented in our laboratory. We summarize the reasons for testing genetic markers after hematopoietic stem cell transplantation, using illustrations from our own experience. This information is designed to help disseminate similar methods in laboratories that do not yet provide such services, to assist laboratory personnel in understanding the clinical and research needs of physicians who request such services and to assist clinicians who interpret the results of genetic marker studies.
Smith
A G
AG
Division of Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.
Martin
P J
PJ
D006085:Graft Survival / Q000235:genetics*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D020080:Tandem Repeat Sequences; D014184:Transplantation, Homologous