Stress response to surgical procedures in the submandibular region and its influence on salivary secretion in mice.
Saliva stimulated by pilocarpine was collected from the mouths of adult ICR male mice after either submandibular-sublingual gland (SM-SL) exposure and SM-SL removal under pentobarbital anaesthesia. Salivary flow rates decreased and salivary protein concentrations increased after both surgical procedures. Serum cortisol was elevated after the operations. Salivary protein separation patterns on sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE) from surgically treated animals were very similar to those from mice given an alpha 1-adrenergic agonist (phenylephrine), but differed significantly from those given a beta-adrenergic agonist (isoproterenol). When the SM-SL was completely removed (i.e. parotid saliva), protein concentrations also dramatically increased. The SDS-PAGE protein separation pattern in the mice whose SM-SL had been removed was very similar to that in those whose SM-SL had been exposed. Hence, a surgical insult in the submandibular region in mice enhances the secretion of salivary protein, mainly in response to alpha 1-adrenergic receptor stimulation in the parotid gland. This study suggests the usefulness of alpha 1-adrenergic receptor-stimulated parotid salivary protein as a marker of surgical insult.
Second Department of Oral and Maxillofacial Surgery, Tsurumi University School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama, 230-8501 Japan.
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Ishii
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Nakagawa
D000316:Adrenergic alpha-Agonists / Q000494:pharmacology; D000818:Animals; D015415:Biomarkers; D004591:Electrophoresis, Polyacrylamide Gel; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010306:Parotid Gland / Q000378:metabolism*; D011942:Receptors, Adrenergic, alpha / Q000502:physiology; D012471:Salivary Proteins and Peptides / Q000096:biosynthesis*; D018709:Statistics, Nonparametric; D013312:Stress, Physiological / Q000378:metabolism*; D013361:Sublingual Gland / Q000601:surgery*; D013363:Submandibular Gland / Q000601:surgery*
D000316:Adrenergic alpha-Agonists; D015415:Biomarkers; D011942:Receptors, Adrenergic, alpha; D012471:Salivary Proteins and Peptides; D007545:Isoproterenol