Langat virus M protein is structurally homologous to prM.
Langat (LGT) virus M protein has been generated in a recombinant system. Antiserum raised against the LGT virus M protein neutralizes tick-borne encephalitis serocomplex flaviviruses but not mosquito-borne flaviviruses, indicating that the M protein is exposed on the surface of virions. The antiserum recognizes intracellular LGT virus prM/M and binds to prM and M in Western blots of whole-cell lysates and purified virus, respectively. These data suggest that the prM and M proteins are structurally similar under native conditions and support the hypothesis that the "pr" portion of prM facilitates proper folding of the M protein for expression on the virion surface.
Center for Tropical Diseases and Department of Pathology, University of Texas Medical Branch, Galveston, Texas 77555-0609, USA.
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Holbrook
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Wang
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Barrett
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology; D015153:Blotting, Western; D002522:Chlorocebus aethiops; D003429:Cross Reactions / Q000276:immunology; D003593:Cytoplasm / Q000821:virology; D004669:Encephalitis Viruses, Tick-Borne / Q000737:chemistry / Q000276:immunology*; D005416:Flavivirus / Q000276:immunology; D007106:Immune Sera / Q000276:immunology; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D016415:Sequence Alignment; D012699:Serology; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000276:immunology*
D000914:Antibodies, Viral; D007106:Immune Sera; D014759:Viral Envelope Proteins; C074932:prM protein, Flavivirus