A novel method for isolation of Brachyspira (Serpulina) hyodysenteriae from pigs with swine dysentery in Italy.
Brachyspira (Serpulina) hyodysenteriae was isolated from 10 of 11 pigs with clinically suspected swine dysentery in six herds in northern Italy. All strains were successfully isolated in the selective blood agar modified medium with spectinomycin and rifampin (BAM-SR) currently used in our laboratory to isolate B. (S.) pilosicoli of human origin, after pre-treatment of intestinal material with spectinomycin and rifampin in foetal calf serum. Isolates had phenotypic characteristics typical of B. (S.) hyodysenteriae.
Department of Pathology and Laboratory Medicine, Section of Microbiology, University of Parma, Viale A. Gramsci, 14-43100, Parma, Italy.
A
A
Calderaro
G
G
Merialdi
S
S
Perini
P
P
Ragni
R
R
Guégan
G
G
Dettori
C
C
Chezzi
D000818:Animals; D017401:Brachyspira hyodysenteriae / Q000302:isolation & purification*; D003470:Culture Media; D004403:Dysentery / Q000382:microbiology / Q000662:veterinary*; D007558:Italy; D010641:Phenotype; D012293:Rifampin; D000198:Spectinomycin; D013145:Spirochaetales Infections / Q000382:microbiology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
D003470:Culture Media; D000198:Spectinomycin; D012293:Rifampin