Is there longitudinal dissociation in the undamaged his bundle? In vitro studies in the normal canine heart.
We investigated the concept of longitudinal dissociation in the His-Purkinje system in vitro. Hearts were excised from the eleven anesthetized dogs and a septal preparation containing the exposed His bundle and the entire right bundle branch and left bundle branch were displayed in a two-dimensional arrangement pinned to the bottom of a superfusion chamber. Tyrodes solution, gassed with 95% O2 and 5% CO2, at 37 degrees C was continuously passed over the preparation. Pacing was performed over a wide range of heart rates (30 to 180/min) from the proximal His bundle and by moving bipolar electrodes we monitored activation at various sites along the right and left bundle branch. The earliest site of muscle activation (27+/-2 msec) on the left septum was a relatively large area in the midposterior septal region; whereas, on the right septum the earliest site of activation (27+/-3 msec) was a relatively small zone at the base of the anterior papillary muscle, (p, N.S). The larger area of early activation on the left compared to the right is consonant with a left to right septal vector accounting for the Q wave in the standard bipolar leads in the normal heart and the loss of Q waves in left bundle branch block. We conclude that His-Purkinje and ventricular muscle activation is remarkably synchronous on both sides of the heart and accounts for optimal contractile function during His bundle or biventricular pacing compared to standard site ventricular pacing, particularly in patients with left ventricular dysfunction.
Cardiac Arrhythmia Research Institute at the University of Oklahoma Health Sciences Center, Oklahoma City 73104, USA. benjamin-scherlag@ouhsc.edu
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Lazzara
D000818:Animals; D002036:Bundle of His / Q000503:physiopathology*; D002304:Cardiac Pacing, Artificial; D004285:Dogs; D004562:Electrocardiography; D066298:In Vitro Techniques; D011690:Purkinje Fibers / Q000503:physiopathology