Detection of hepatitis C virus RNA sequences in hepatic portal cholangiocarcinoma tissue by reverse transcription polymerase chain reaction.
OBJECTIVE
To detect hepatitis C virus (HCV) RNA sequences in the hepatic portal cholangiocarcinoma tissues and their relationship.


METHODS
RNA was extracted from paraffin-embedded cholangiocarcinoma tissues of 6 patients by guanidinium method, subjected to reverse transcription and then amplified by double PCR technique using nested primers from the highly conserved 5' noncoding region of HCV genome.


RESULTS
HCV RNA of 5' NT sequences was found in the hepatic portal cholangiocarcinoma tissues of 5 out of 6 (83%) patients.


CONCLUSIONS
HCV RNA sequences present with high infectious rate in cholangiocarcinoma, and reverse transcription polymerase chain reaction (RT-PCR) assay using primers derived from 5' NT region of HCV sequence is most useful in detecting HCV infection. The development of cholangiocarcinoma awaits further studies.
Department of Surgery, Fujian Medical University Union Hospital, Inistitute of Hepatobiliary Surgery, Fuzhou 350001.
F
F
Yin
B
B
Chen
D000230:Adenocarcinoma / Q000821:virology; D000231:Adenocarcinoma, Papillary / Q000821:virology; D000328:Adult; D001650:Bile Duct Neoplasms / Q000821:virology*; D001653:Bile Ducts, Intrahepatic / Q000821:virology*; D018281:Cholangiocarcinoma / Q000821:virology*; D005260:Female; D016174:Hepacivirus / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017423:Sequence Analysis, RNA
D012367:RNA, Viral