[Construction of a bacterial artificial chromosome (BAC) contig encompassing the bacterial blight resistance gene Xa4 locus in rice].
The gene Xa4 confers dominantly resistance to rice bacterial blight, which has been finely mapped between RFLP markers G181 and L1044, and co-segregated with the resistance gene homologues sequence marker RS13. The three markers were used to screen a rice Bacterial Artificial Chromosome (BAC) library constructed from IRBB56, a Xa4-harborring indica variety, resulting in the detection of totally 128 positive clones. Of the 18 positive clones picked out by RS13, 4 and 6 clones were simultaneously detected by G181 and L1044, respectively. Based on their HindIII restriction patterns, 12 clones were selected out to construct a contig that spanned about 420 kb covering the Xa4 locus, which is a solid base for the isolation of Xa4 gene.
Jiang
G H
GH
Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China.
Wang
W M
WM
Xie
B
B
Zhai
W X
WX
Lu
R L
RL
Zhu
L H
LH
D022202:Chromosomes, Artificial, Bacterial; D012275:Oryza / Q000235:genetics*; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology