Hematological studies on black cumin oil from the seeds of Nigella sativa L.
The methanol soluble portion of black cumin oil, which is prepared by compression of seeds of Nigella sativa L., showed inhibitory effects on arachidonic acid (AA)-induced platelet aggregation and blood coagulation. By bioactive assay of AA-induced platelet aggregation, the methanol soluble part was purified to isolate a new compound 2-(2-methoxypropyl)-5-methyl-1,4-benzenediol (1) and two known compounds, thymol (2), carvacrol (3), having very strong inhibitory activity. Further, we then examined the isolated compounds (1-3) and eight related compounds by the screening test for AA-induced platelet aggregation. Compounds possessing aromatic hydroxyl and acetoxyl group had more potent activity than aspirin, which is well known as a remedy for thrombosis.
Department of Natural Medicine and Phytochemistry, Meiji Pharmaceutical University, Tokyo, Japan.
S
S
Enomoto
R
R
Asano
Y
Y
Iwahori
T
T
Narui
Y
Y
Okada
A N
AN
Singab
T
T
Okuyama
D000818:Animals; D019661:Apiaceae / Q000737:chemistry*; D001777:Blood Coagulation / Q000187:drug effects; D005342:Fibrinolysis / Q000187:drug effects; D005343:Fibrinolytic Agents / Q000737:chemistry / Q000494:pharmacology*; D008401:Gas Chromatography-Mass Spectrometry; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D009904:Optical Rotation; D010946:Plants, Medicinal / Q000737:chemistry*; D010974:Platelet Aggregation / Q000187:drug effects; D011817:Rabbits; D012639:Seeds / Q000737:chemistry
D005343:Fibrinolytic Agents