Quantitative determination of L-arginine by enzymatic end-point analysis.
An enzymatic end-point method for the quantitative determination of L-arginine was evaluated with samples of synthetic wine and natural grape juice. The enzymes arginase, urease, and glutamate dehydrogenase were used in this simple assay, similar to those described for many metabolites by Boehringer-Mannheim. In synthetic wine, recovery of L-arginine ranged between 98.3 and 104.4% and the precision as coefficient of variation was between 0.4 and 1.47% in the concentration range of the method, 0-100 mg/L L-arginine. The recovery of L-arginine in a grape juice with added L-arginine after clarification with polyvinylpolypyrrolidone ranged between 100 and 101.3%, and the coefficient of variation was 0.6%. The method has low material costs of approximately 0.43 U.S.$ per assay, and the time course of the reaction facilitates measurement of several samples concurrently. The results of this evaluation indicate that the enzymatic assay is a preferred method over colorimetric methods for the manual determination of L-arginine.
Institute of Molecular BioSciences, Massey University, Private Bag 11222, Palmerston North, New Zealand. r.mira@massey.ac.nz
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Mira de Orduña
D001119:Arginase; D001120:Arginine / Q000032:analysis*; D001628:Beverages / Q000032:analysis*; D003365:Costs and Cost Analysis; D005969:Glutamate Dehydrogenase; D009520:New Zealand; D015203:Reproducibility of Results; D019685:Rosales; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000191:economics / Q000379:methods; D014481:United States; D014510:Urease; D014920:Wine / Q000032:analysis*
D001120:Arginine; D005969:Glutamate Dehydrogenase; D014510:Urease; D001119:Arginase