[Identification of respiratory syncytial virus and adenovirus in children with chronic serous otitis].
OBJECTIVE
To study the presence of respiratory syncytial virus (RSV) and adenovirus in the effusions of the middle ear and adenoid tissue from patients with chronic otitis media with effusion by use of the polymerase chain reaction.


METHODS
The effusions and adenoid samples were collected from 32 children undergoing myringotomy and ventilation tube placement for chronic otitis media with effusion. The samples were separated for viral culture, immunofluorescence and PCR analysis.


RESULTS
One (3%) effusion sample was positive for adenovirus by the PCR. None of samples (effusion and adenoid) were positives for RSV. The results of viral culture and immunofluorescence were all negative.


CONCLUSIONS
Our results can not support that respiratory viruses play a role in the pathogenesis of chronic otitis media with effusion.
Departamento de ORL, Hospital Central de Asturias, Celestino Villamil s/n. 33006 Oviedo.
E
E
Maseda
J L
JL
Llorente
S
S
Melón
M
M
Oña
M
M
Moro
C
C
Manrique
C
C
Suarez
D000256:Adenoviridae / Q000302:isolation & purification*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D008297:Male; D010034:Otitis Media with Effusion / Q000821:virology*; D016133:Polymerase Chain Reaction; D018113:Respiratory Syncytial Virus, Human / Q000302:isolation & purification*