Identification of a novel HLA-B allele--HLA-B*4902.
We report herein the identification of HLA-B*4902. This new allele was identified in a Caucasian individual serologically typed as B49. The allele codifying for this antigen was not clearly detectable with polymerase chain reaction using sequence-specific primers (PCR-SSP) because of an atypical amplification pattern. DNA sequencing demonstrated the presence of a new variant due to two nucleotide substitutions (from G to C and from T to C) in exon 2 at nucleotides 309 and 311 respectively. These substitutions would result in a silent mutation and in one amino acid substitution from Ile to Thr, respectively.
Centro Trasfusionale e di Immunologia dei Trapianti, Ospedale Maggiore Policlinico IRCCS, Milan, Italy. nitpmi@polic.cilea.it
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Poli
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Longhi
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Frison
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Scalamogna
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Crespiatico
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Bianchi
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Sirchia
D000483:Alleles; D001483:Base Sequence; D005260:Female; D015235:HLA-B Antigens / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D017354:Point Mutation; D044465:White People / Q000235:genetics
D015235:HLA-B Antigens; C436267:HLA-B*49:02 antigen