Composition and chirality of amino acids in aerosol/dust from laboratory and residential enclosures.
Initial results from the analyses of geological and anthropological samples for amino acids were difficult to accept because of the high enantiomeric purities of the analytes (i.e., predominantly L-amino acids). Consequently, sources of contamination had to be considered. All sources were eliminated except for direct atmospheric contamination. Essentially invisible, microscopic, aerosol/dust was found to rapidly contaminate the surface of samples and sample containers even after brief exposure times in clean laboratories. Contamination increased with exposure time. The aerosol/dust amino acids were contained predominantly in a proteinaceous material. Aerosol/dust from different locations can contain different percentages of proteinoid/amino acid material. However, the relative concentrations of the amino acids were similar for both laboratory and residential samples. The enantiomeric purity of the L-amino acids studied in aerosol/dust appears to be 99% or greater for the samples examined. Thus, even slight contamination of any sample with microscopic dust or aerosol particles can skew the results of trace amino acid analyses and amino acid e.e. determinations.
Iowa State University, Department of Chemistry, Gilman Hall, Ames, Iowa, USA.
D W
DW
Armstrong
J P
JP
Kullman
X
X
Chen
M
M
Rowe
D000336:Aerosols; D016902:Air Pollution, Indoor / Q000009:adverse effects*; D000596:Amino Acids / Q000737:chemistry* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D004391:Dust; D007753:Laboratories; D013237:Stereoisomerism
D000336:Aerosols; D000596:Amino Acids; D004391:Dust