A reduction of tumor necrosis factor-alpha in paw exudate of lipopolysaccharide treated rats by nimesulide.
TNF-alpha is considered to play a pivotal role in many inflammatory and illness states. In our previous study we found that nimesulide, a COX-2 selective inhibitor, prevents the lipopolysaccharide-induced elevation in plasma TNF-alpha in rats. The present study was undertaken to elucidate the effect of nimesulide on TNF-alpha levels in the paw exudate of rats pretreated with lipopolysaccharide (LPS). Rats were injected (subplantar) with LPS (100 microg/paw) in the right hind, which resulted in a prominent increase in paw exudate TNF-alpha levels, peaked at one hour post injection. In rats pretreated with nimesulide (30 mg/kg, i.p.), the elevation in TNF-alpha in the paw exudate was significantly reduced, as compared to the LPS treated group. These results further stress the multiple antiinflammatory effects of nimesulide.
Department of Clinical Pharmacology, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
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Azab
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Kaplanski
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002351:Carrageenan; D015232:Dinoprostone / Q000378:metabolism; D004487:Edema / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D005122:Exudates and Transudates / Q000187:drug effects / Q000378:metabolism*; D007213:Indomethacin / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013449:Sulfonamides / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D008070:Lipopolysaccharides; D013449:Sulfonamides; D014409:Tumor Necrosis Factor-alpha; D002351:Carrageenan; D015232:Dinoprostone; C012655:nimesulide; D007213:Indomethacin