Evaluation of 8-hydroxydeoxyguanosine in human oral cells: the importance of tobacco smoke and urban environment.
The DNA adduct 8-hydroxydeoxyguanosine (8-OHdG) has been widely used as a sensitive biomarker for oxidative damage. To investigate the role of environmental factors on oxidative DNA damage formation, the level of 8-OHdG was determined in oral cells from 109 healthy volunteers by an immunohistochemical method. A statistically significantly higher content of 8-OHdG was detected in oral cells from smokers (111 +/- 55, n = 38) compared with non smokers (78 +/- 48, n = 71), (p < 0.01). Moreover, subjects living in an urban area showed a higher level of oxidative damage with respect to those living in a countryside-suburban area (99 +/- 53, n = 58 vs. 78 +/- 51, n = 51), (p = 0.03). No significant association was detected between 8-OHdG in oral cells and other variables such as passive smoke, oral infections, alcohol or vitamin intake and grilled food consumption. This work suggests that tobacco smoke and environmental exposure to pollutants lead to a measurable increase of oxidative damage in oral cells and confirms that the immunoperoxidase method is an appropriate approach for epidemiological analyses.
Giovanni XXIII Cancer Research Center, Institute of General Pathology, Catholic University School of Medicine, L.go F.Vito, 1, 00168 Rome, Italy. crogxxiii@rm.unicatt.it
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Romano
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Sgambato
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Flamini
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Boninsegna
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Milito
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Ardito
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Cittadini
D000080242:8-Hydroxy-2'-Deoxyguanosine / Q000031:analogs & derivatives; D000293:Adolescent; D000328:Adult; D000428:Alcohol Drinking; D015415:Biomarkers / Q000032:analysis; D004249:DNA Damage; D004032:Diet; D004777:Environment; D005260:Female; D006147:Guanine / Q000031:analogs & derivatives* / Q000032:analysis*; D006801:Humans; D007558:Italy; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000166:cytology* / Q000473:pathology; D012424:Rural Population; D012907:Smoking / Q000473:pathology*; D013383:Suburban Population; D014028:Tobacco Smoke Pollution; D014505:Urban Population; D014815:Vitamins
D015415:Biomarkers; D014028:Tobacco Smoke Pollution; D014815:Vitamins; D006147:Guanine; C078307:8-oxo-7,8-dihydrodeoxyguanine; D000080242:8-Hydroxy-2'-Deoxyguanosine