A calcium-binding protein with four EF-hand motifs in Streptomyces ambofaciens.
A gene (cabA) encoding a calcium-binding protein was cloned from Streptomyces ambofaciens. CabA was 180 amino acid residues long and contained four typical EF-hand motifs bearing high sequence similarity to the calcium-binding sites in calmodulin. Consistent with this, CabA showed distinct calcium-binding activity, comparable to bovine brain calmodulin. cabA was transcribed throughout growth, as found by S1 nuclease mapping. Southern hybridization experiments showed that a single copy of cabA was present in various Streptomyces species. A hypothetical relationship between CabA and aerial mycelium formation in this strain was examined, since S. ambofaciens showed calcium-dependent aerial mycelium formation. However, disruption of cabA or overexpression of cabA in S. ambofaciens caused no detectable phenotypic changes.
Yonekawa
T
T
Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Japan.
Ohnishi
Y
Y
Horinouchi
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins; D015139:Blotting, Southern; D002118:Calcium / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D002147:Calmodulin / Q000235:genetics / Q000378:metabolism; D002417:Cattle; D020832:EF Hand Motifs / Q000235:genetics; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism; D013302:Streptomyces / Q000737:chemistry* / Q000235:genetics
D001426:Bacterial Proteins; C432589:CabA protein, Streptomyces ambofaciens; D002135:Calcium-Binding Proteins; D002147:Calmodulin; D015719:Single-Strand Specific DNA and RNA Endonucleases; D002118:Calcium