Synthesis and properties of peptide nucleic acids containing a psoralen unit.
We prepared the psoralen PNA unit from 8-methoxypsoralen and synthesized various PNAs containing psoralen by a typical (t)()Boc method. PNAs containing psoralen (P-PNA) at strand end formed a stable duplex with complementary DNA. The hybridization of P-PNA with complementary DNA resulted in a considerable decrease of the psoralen fluorescence.
Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, CREST, Japan Science and Technology Corporation (JST), Kyoto 606-8501, Japan.
A
A
Okamoto
K
K
Tanabe
I
I
Saito
D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry; D011564:Furocoumarins; D007202:Indicators and Reagents; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis* / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D018076:DNA, Complementary; D011564:Furocoumarins; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D020135:Peptide Nucleic Acids