Identification of barley genome segments introgressed into wheat using PCR markers.
Barley has several important traits that might be used in the genetic improvement of wheat. For this report, we have produced wheat-barley recombinants involving barley chromosomes 4 (4H) and 7 (5H). Wheat-barley disomic addition lines were crossed with 'Chinese Spring' wheat carrying the phlb mutation to promote homoeologous pairing. Selection was performed using polymerase chain reaction (PCR) markers to identify lines with the barley chromosome in the ph1b background. These lines were self pollinated, and recombinants were identified using sequence-tagged-site (STS) primer sets that allowed differentiation between barley and wheat chromosomes. Several recombinant lines were isolated that involved different STS-PCR markers. Recombination was confirmed by allowing the lines to self pollinate and rescreening the progeny via STS-PCR. Progeny testing confirmed 9 recombinants involving barley chromosome 4 (4H) and 11 recombinants involving barley chromosome 7 (5H). Some recombinants were observed cytologically to eliminate the possibility of broken chromosomes. Since transmission of the recombinant chromosomes was lower than expected and since seed set was reduced in recombinant lines, the utility of producing recombinants with this method is uncertain.
Sherman
J D
JD
Department of Plant Sciences, Montana State University, Bozeman 59717, USA. jsherman@montana.edu
Smith
L Y
LY
Blake
T K
TK
Talbert
L E
LE
D018744:DNA, Plant; D004591:Electrophoresis, Polyacrylamide Gel; D005819:Genetic Markers; D018745:Genome, Plant; D001467:Hordeum / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D016324:Sequence Tagged Sites; D014908:Triticum / Q000235:genetics*
D018744:DNA, Plant; D005819:Genetic Markers