A cis-dominant cyclic nucleotide-dependent regulatory domain in the 3'-untranslated region of Na(+)/glucose cotransporter (SGLT1) mRNA.
A 122 nt uridine-rich sequence (URE) in the Na(+)/glucose cotransporter (SGLT1) mRNA 3'-untranslated region is critical for cAMP-dependent message stabilization. Its function was investigated in LLC-PK(1) cells stably expressing beta-globin reporter transcripts. Insertion of the SGLT1 URE downstream from an unrelated destabilizing sequence, the c-fos ARE, evoked cAMP-dependent message stabilization. Stabilization was blocked by a substitution mutation within the SLGT1 URE. These observations indicate that the SGLT1 URE is sufficient to transmit cAMP-dependent, cis-dominant mRNA stabilization in the presence of appropriate trans-acting factors and appears to function independently of the nature of the destabilizing domain.
Department of Biochemistry and Molecular Biology, University of Texas-Houston Medical School, P.O. Box 20708, Houston, TX 77225, USA.
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Loflin
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Lever
D020413:3' Untranslated Regions / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism*; D005786:Gene Expression Regulation; D008562:Membrane Glycoproteins / Q000235:genetics*; D009004:Monosaccharide Transport Proteins / Q000235:genetics*; D009690:Nucleic Acid Conformation; D020871:RNA Stability / Q000502:physiology*; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism; D051269:Sodium-Glucose Transporter 1; D013552:Swine; D014529:Uridine / Q000737:chemistry
D020413:3' Untranslated Regions; D008562:Membrane Glycoproteins; D009004:Monosaccharide Transport Proteins; D012333:RNA, Messenger; D051269:Sodium-Glucose Transporter 1; D000242:Cyclic AMP; D014529:Uridine