Synthesis and characterization of fluorescent cobalamin (CobalaFluor) derivatives for imaging.
Fluorescent derivatives of cobalamin have been prepared by linking fluorophores to cobalamin through a propylamide spacer. Fluorescein, naphthofluorescein, and Oregon Green derivatives have been prepared in good yield by reaction of the fluorophore NHS-ester with beta-(3-aminopropyl)cobalamin to form fluorescent cobalamin conjugates (CobalaFluors) that are potentially suitable for the in vitro and in vivo imaging of transcobalamin receptors on cancer cells.
Department of Chemistry, University of Utah, 315 S. 1400 E., Salt Lake City, Utah 84112-0850, USA.
C C
CC
Smeltzer
M J
MJ
Cannon
P R
PR
Pinson
J D
JD
Munger
F G
FG
West
C B
CB
Grissom
D001896:Boron Compounds; D005452:Fluoresceins / Q000138:chemical synthesis* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D007202:Indicators and Reagents; D008968:Molecular Conformation; D015394:Molecular Structure; D009369:Neoplasms / Q000175:diagnosis; D011956:Receptors, Cell Surface / Q000032:analysis; D013053:Spectrophotometry; D014805:Vitamin B 12 / Q000138:chemical synthesis* / Q000737:chemistry
D001896:Boron Compounds; D005452:Fluoresceins; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D011956:Receptors, Cell Surface; C426407:n-fluoroacyl oxazaborolidine; C000601316:naphthofluorescein; C028176:transcobalamin receptor; D014805:Vitamin B 12