Improved renal preservation with PB-3 flush solution during 72 h of cold storage.
Previously, PB-2 flush solution has been found to be superior to Collins 2 solution (C-2) in extending renal viability in the dog. To further characterize preservation mechanisms, we studied mitochondrial oxidative function during 72 h of cold storage comparing PB-3, UW-1, and C-2 flush storage solutions. Complex 1 dependent mitochondrial oxidative phosphorylation (MOP) was found to be significantly less (P < 0.001) at 5 h and 72 h of cold storage (P < 0.001) for the C-2 compared to the other flush groups. Complex 2 dependent MOP had parallel results, having significantly less function (P < 0.002) at 5 h and 72 h (P < 0.02) of cold storage in the C-2 group compared to the other groups. PB-3 and UW-1 solutions were noted to be comparable, suggesting possible equivalent preservation efficacy. Nevertheless the components of PB-3 at the level of MOP contributed significantly to better preservation compared to C-2 solution.
USCF Transplants, San Francisco, CA 94143, USA.
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Bretan
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Sharma
D000227:Adenine Nucleotides; D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D003080:Cold Temperature; D007668:Kidney / Q000378:metabolism; D008297:Male; D008353:Mannitol; D008928:Mitochondria / Q000378:metabolism; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D010085:Oxidative Phosphorylation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014700:Verapamil
D000227:Adenine Nucleotides; D019188:Organ Preservation Solutions; C422852:PB-3 flush solution; D008353:Mannitol; D000493:Allopurinol; D014700:Verapamil; D000241:Adenosine