Plasmids with the Cre-recombinase and the dominant nat marker, suitable for use in prototrophic strains of Saccharomyces cerevisiae and Kluyveromyces lactis.
Two plasmids are described which can be used to remove the "loxP-markerMX-loxP" cassettes in strains lacking the ura3 mutation. Both contain the Cre-recombinase under control of the GAL1 promoter and the natMX cassette with the dominant marker nat, which gives yeasts resistance to the antibiotic ClonNat. pNatCre contains ARSH and CEN6 for maintenance in Saccharomyces cerevisiae. pKlNatCre has a Kluyveromyces lactis replication origin and centromere in addition.
Institute for Molecular Plant Sciences, Clusius Laboratory, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands. Steensma@RULBIM.Leiden.Univ.nl
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Ter Linde
D000123:Acetyltransferases / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D019426:Integrases / Q000235:genetics*; D007716:Kluyveromyces / Q000201:enzymology / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D014764:Viral Proteins
D005819:Genetic Markers; D014764:Viral Proteins; D000123:Acetyltransferases; C061313:protein N-terminal acetyltransferase; C045073:Cre recombinase; D019426:Integrases