The Tctex1/Tctex2 class of dynein light chains. Dimerization, differential expression, and interaction with the LC8 protein family.
The Tctex1/Tctex2 family of dynein light chains associates with the intermediate chains at the base of the soluble dynein particle. These components are essential for dynein assembly and participate in specific motor-cargo interactions. To further address the role of these light chains in dynein activity, the structural and biochemical properties of several members of this polypeptide class were examined. Gel filtration chromatography and native gel electrophoresis indicate that recombinant Chlamydomonas flagellar Tctex1 exists as a dimer in solution. Furthermore, yeast two-hybrid analysis suggests that this association also occurs in vivo. In contrast, both murine and Chlamydomonas Tctex2 are monomeric. To investigate protein-protein interactions involving these light chains, outer arm dynein from Chlamydomonas flagella was cross-linked using dimethylpimelimidate. Immunoblot analysis of the resulting products revealed the interaction of LC2 (Tctex2) with LC6, which is closely related to the highly conserved LC8 protein found in many enzyme systems, including dynein. Northern dot blot analysis demonstrated that Tctex1/Tctex2 family light chains are differentially expressed both in a tissue-specific and developmentally regulated manner in humans. These data provide further support for the existence of functionally distinct populations of cytoplasmic dynein with differing light chain content.
DiBella
L M
LM
Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06030-3305, USA.
Benashski
S E
SE
Tedford
H W
HW
Harrison
A
A
Patel-King
R S
RS
King
S M
SM
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000737:chemistry*; D002696:Chlamydomonas / Q000737:chemistry; D002850:Chromatography, Gel; D002942:Circular Dichroism; D003432:Cross-Linking Reagents / Q000494:pharmacology; D003593:Cytoplasm / Q000378:metabolism; D019281:Dimerization; D029721:Drosophila Proteins; D004398:Dyneins / Q000737:chemistry* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D007096:Imidoesters / Q000494:pharmacology; D015151:Immunoblotting; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D008868:Microtubule Proteins / Q000737:chemistry*; D008869:Microtubule-Associated Proteins; D008954:Models, Biological; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry*; D010802:Phylogeny; D011485:Protein Binding; D011994:Recombinant Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D020798:Two-Hybrid System Techniques; D044767:Ubiquitin-Protein Ligases; D056427:t-Complex Genome Region
D002352:Carrier Proteins; D003432:Cross-Linking Reagents; D029721:Drosophila Proteins; C533386:Dynlt1b protein, mouse; D007096:Imidoesters; D047908:Intracellular Signaling Peptides and Proteins; D008868:Microtubule Proteins; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; D011994:Recombinant Proteins; C533389:Tcte3 protein, mouse; C029326:dimethyl pimelimidate; C116196:PPP1R11 protein, human; D044767:Ubiquitin-Protein Ligases; D004398:Dyneins