Regulation of the expression of endopolygalacturonase gene PGU1 in Saccharomyces.
Previous work in our laboratory has shown that Saccharomyces bayanus strain SCPP is the only reported yeast expressing the three types of pectolytic enzymes: pectin esterases, pectin lyases and polygalacturonases. One of these enzymes, the endopolygalacturonase (endoPG), hydrolyses plant-specific polysaccharide pectin. The endoPG encoding gene (PGU1) is also present in Saccharomyces cerevisiae. It has been shown that this endoPG is required for the development of pseudohyphae. Using genomic DNA, the PGU1-1 and PGU1-2 promoters of these strains have been amplified and used to construct gene fusions with the beta-galactosidase gene. On the basis of beta-galactosidase measurements, we compared the expression of both promoters in different environmental conditions in order to identify their modulation. We have shown that the PGU1 gene is upregulated by the presence of the pectin and the product resulting from endopolygalacturonase activity. Moreover, expression of the PGU1 is also enhanced under respiratory and filament formation conditions.
Université de Reims, Faculté des Sciences, Laboratoire de Microbiologie Générale et Moléculaire, Europol'Agro, B.P. 1039, 51687 Reims cedex 2, France.
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Gognies
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Simon
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Belarbi
D001483:Base Sequence; D004271:DNA, Fungal / Q000737:chemistry; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D008969:Molecular Sequence Data; D010368:Pectins / Q000737:chemistry; D011096:Polygalacturonase / Q000096:biosynthesis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012440:Saccharomyces / Q000201:enzymology* / Q000235:genetics*; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
D004271:DNA, Fungal; D010368:Pectins; D011096:Polygalacturonase