Graft endothelium and thickness after penetrating keratoplasty, comparing mechanical and excimer laser trephination: a prospective randomised study.
OBJECTIVE
To assess the impact of nonmechanical trephination on the graft endothelium and thickness after penetrating keratoplasty (PK).


METHODS
Inclusion criteria for this prospective, randomised, cross-sectional, clinical study were: (1) Treatment between October 1992 and December 1997; (2) one surgeon (G.O.H.N.); (3) primary central PK; (4) Fuchs' dystrophy (diameter 7.5/7.6 mm) or keratoconus (diameter 8.0/8.1 mm); (5) graft oversize 0.1 mm; (6) no previous intraocular surgery; (7) 16-bite double-running diagonal suture. In 179 patients (mean age 51+/-18 years), PK was performed using either the 193-nm Meditec MEL60 excimer laser ("Excimer") along metal masks with eight "orientation teeth/notches" (53 keratoconus, 35 Fuchs' dystrophy) or motor trephination with the Mikrokeratron (Geuder) ("Control": 53 keratoconus, 38 Fuchs' dystrophy). For donor trephination from the epithelial side an artificial anterior chamber was used in both groups. In 27% of the excimer and 29% of the control group a triple procedure was performed. Specular microscopy (EM-1000, Tomey) and pachymetry (SP-2000, Tomey) were performed before removal of the first suture (0.4+/-0.2 years postoperatively), before (1.1+/-0.4 years) and after (1.7+/-0.6 years) removal of the second suture but before any additional surgical intervention.


RESULTS
Endothelial cell count: Neither "two-sutures-in" (1953+/-426/1804+/-385 cells/mm2, p=0.13), "one-suture-in" (1629+/-439/1765+/-440 cells/mm2, p=0.27), nor "all-sutures-out" (1259+/-493/1294+/-532 cells/mm2, p=0.83) differed significantly between Excimer and Control. Graft thickness: Neither "two-sutures-in" (527+/-58/524+/-16 mucrom, p=0.89), "one-suture-in" (537+/-72/551+/-40 microm, p=0.86), nor "all-sutures-out" (576+/-53/565+/-62 microm, p=0.38) differed significantly between Excimer and Control. Cell count and corneal thickness were not significantly different comparing Fuchs' dystrophy and keratoconus or comparing PK only and triple procedures. Graft thickness and endothelial cell count correlated highly significantly inversely with "all sutures out" (P<0.0001).


CONCLUSIONS
Excimer laser trephination from the epithelial side using an artificial anterior chamber in donors seems to have no disadvantages concerning the graft endothelium after PK. Endothelial cell loss was not increased in eyes with Fuchs' dystrophy compared with keratoconus or after triple procedures compared with PK only.
Seitz
B
B
Department of Ophthalmology, University of Erlangen-Nürnberg, Germany. berthold.seitz@augen.imed.uni-erlangen.de
Langenbucher
A
A
Nguyen
N X
NX
Kus
M M
MM
Küchle
M
M
Naumann
G O
GO
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000886:Anthropometry; D002452:Cell Count; D003430:Cross-Sectional Studies; D004728:Endothelium, Corneal / Q000033:anatomy & histology*; D005260:Female; D005642:Fuchs' Endothelial Dystrophy / Q000601:surgery*; D006801:Humans; D007640:Keratoconus / Q000601:surgery*; D015948:Keratoplasty, Penetrating / Q000379:methods*; D053685:Laser Therapy / Q000379:methods*; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D013536:Suture Techniques; D014019:Tissue Donors