Enhanced acid sensitivity of pressure-damaged Escherichia coli O157 cells.
Pressure-damaged Escherichia coli O157 cells were more acid sensitive than native cells and were impaired in pH homeostasis. However differences in acid sensitivity were not related to differences in cytoplasmic pH (pH(i)). Cellular beta-galactosidase was more acid labile in damaged cells. Sensitization to acid may thus involve loss of protective or repair functions.
Pagán
R
R
Food Microbial Sciences Unit, School of Food Biosciences, University of Reading, Whiteknights, Reading RG6 6AP, United Kingdom.
Jordan
S
S
Benito
A
A
Mackey
B
B
D015169:Colony Count, Microbial; D019453:Escherichia coli O157 / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development*; D006851:Hydrochloric Acid / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D006874:Hydrostatic Pressure; D001616:beta-Galactosidase / Q000378:metabolism
D001616:beta-Galactosidase; D006851:Hydrochloric Acid