Vitamin requirements of the cultured flesh fly cells, Sarcophaga peregrina (Diptera, Sarcophagidae).
Essential vitamins for the growth of a cell line derived from the flesh fly, Sarcophaga peregrina, were determined. By examining the survivability of continuous passages of the cells in the chemically defined medium lacking one vitamin, thiamine, riboflavin, pantothenate and either niacin or niacinamide were found to be essential for the continuous growth of the flesh fly cells in vitro. [Originally published in Volume 37, Archives of Insect Biochemistry and Physiology, 37:283-286 (1998).]
Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan.
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Mitsuhashi
D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line; D004175:Diptera / Q000254:growth & development* / Q000378:metabolism; D009525:Niacin / Q000378:metabolism; D009536:Niacinamide / Q000378:metabolism; D010205:Pantothenic Acid / Q000378:metabolism; D012256:Riboflavin / Q000378:metabolism; D013831:Thiamine / Q000378:metabolism; D014815:Vitamins / Q000378:metabolism*
D014815:Vitamins; D010205:Pantothenic Acid; D009536:Niacinamide; D009525:Niacin; D012256:Riboflavin; D013831:Thiamine