Chiroptical detection during liquid chromatography 7. The rotation angle/absorbance ratio of chiral molecules. Its possible use for on-line analysis during preparative separations of enantiomers.
The rotation angle/absorbance ratios C+ = alpha+/A+ and C- = a-/A-, determined via detection by a polarimeter and a photometer, were checked for the first time with reference to their use for on-line analysis during preparative separations. For this purpose, (+)-, (-)- and (+/-)-carvones were investigated by liquid chromatography (LC) on microcrystalline tribenzoylcellulose. It turned out that the ratios C+ and C- depend only slightly upon concentration (Table 1). Overlapped peaks of enantiomers were successfully submitted to computer deconvolution (e.g. Fig. 2, bottom). A procedure for on-line analysis during preparative LC is proposed.
Institut für Organische Chemie, Universität Regensburg, Germany.
F
F
Brandl
N
N
Pustet
A
A
Mannschreck
D002853:Chromatography, Liquid / Q000379:methods*; D000081005:Cyclohexane Monoterpenes; D004563:Electrochemistry; D039821:Monoterpenes; D055095:Optics and Photonics; D013237:Stereoisomerism; D013729:Terpenes / Q000032:analysis*
D000081005:Cyclohexane Monoterpenes; D039821:Monoterpenes; D013729:Terpenes; C006923:carvone