Towards a protein interaction map of potyviruses: protein interaction matrixes of two potyviruses based on the yeast two-hybrid system.
A map for the interactions of the major proteins from Potato virus A (PVA) and Pea seed-borne mosaic virus (PSbMV) (members of the genus POTYVIRUS:, family POTYVIRIDAE:) was generated using the yeast two-hybrid system (YTHS). Interactions were readily detected with five PVA protein combinations (HC-HC, HC-CI, VPg-VPg, NIa-NIb and CP-CP) and weak but reproducible interactions were detected for seven additional combinations (P1-CI, P3-NIb, NIaPro-NIb, VPg-NIa, VPg-NIaPro, NIaPro-NIa and NIa-NIa). In PSbMV, readily detectable interactions were found in five protein combinations (HC-HC, VPg-VPg, VPg-NIa, NIa-NIa and NIa-NIb) and weaker but reproducible interactions were detected for three additional combinations (P3-NIa, NIa-NIaPro and CP-CP). The self-interactions of HC, VPg, NIa and CP and the interactions of VPg-NIa, NIa-NIaPro and NIa-NIb were, therefore, common for the two potyviruses. The multiple protein interactions revealed in this study shed light on the co-ordinated functions of potyviral proteins involved in virus movement and replication.
Guo
Deyin
D
Institute of Biotechnology, PO Box 56 (Viikinkaari 9), Viikki Biocenter, FIN-00014 University of Helsinki, Finland1.
Rajamäki
Minna-Liisa
ML
Department of Plant Biology, Genetics Centre, SLU, S-75007 Uppsala, Sweden2.|Institute of Biotechnology, PO Box 56 (Viikinkaari 9), Viikki Biocenter, FIN-00014 University of Helsinki, Finland1.
Saarma
Mart
M
Institute of Biotechnology, PO Box 56 (Viikinkaari 9), Viikki Biocenter, FIN-00014 University of Helsinki, Finland1.
Valkonen
Jari P T
JPT
Department of Plant Biology, Genetics Centre, SLU, S-75007 Uppsala, Sweden2.|Institute of Biotechnology, PO Box 56 (Viikinkaari 9), Viikki Biocenter, FIN-00014 University of Helsinki, Finland1.
D006824:Hybridization, Genetic; D017800:Potyvirus / Q000737:chemistry*; D014764:Viral Proteins / Q000737:chemistry* / Q000502:physiology; D014779:Virus Replication; D015003:Yeasts / Q000235:genetics
D014764:Viral Proteins