Nested polymerase chain reaction for amplification of the Cryptosporidium oocyst wall protein gene.
We developed a sensitive nested polymerase chain reaction procedure for the Cryptosporidium oocyst wall protein (COWP) gene. Amplification and genotyping were successful in 95.2% of 1,680 fecal samples, 77.6% by the unnested and 17.6% by the nested COWP procedure. The COWP gene was amplified from 2,128 fecal samples: 71 from livestock animals and 2,057 from humans. This series included 706 cases from seven drinking water-associated outbreaks and 51 cases from five swimming pool-associated outbreaks, as well as 1,300 sporadic cases.
PHLS Central Public Health Laboratory, London, UK.
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Pedraza-Díaz
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Amar
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Nichols
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McLauchlin
D000818:Animals; D003458:Cryptosporidium / Q000254:growth & development; D005838:Genotype; D016133:Polymerase Chain Reaction; D015800:Protozoan Proteins / Q000235:genetics*; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D014867:Water / Q000469:parasitology; D014881:Water Supply
D015800:Protozoan Proteins; D012337:RNA, Ribosomal, 18S; D014867:Water