Inhibitory effect of dopamine on Ca(2+)-calmodulin-dependent protein kinase II activity in rat hippocampal slices.
OBJECTIVE
To study the effect of dopamine (DA) on Ca(2+)-calmodulin dependent protein kinase II (CCDPK II) activity in rat hippocampus.


METHODS
Using rat hippocampal slices as an in vitro model, the activity of CCDPK II was examined by the method of 32P-incorporation.


RESULTS
Exogenous DA reduced CCDPK II activity in hippocampal slices in a concentration- and time-dependent manner. Removal of extracellular calcium antagonized the DA-induced inhibition of CCDPK II activity, partially or completely. The activity of CCDPK II was markedly decreased by apomorphine (a nonselective DA receptor agonist), SKF38393 (a selective D1-like DA receptor agonist), or quinpirole (a selective D2-like DA receptor agonist). The inhibition of CCDPK II activity induced by exogenous DA was abolished by preincubation with Sch-23390, a selective D1-like DA receptor antagonist, or domperidone, a selective D2-like DA receptor antagonist.


CONCLUSIONS
DA has an inhibitory effect on CCDPK II activity in rat hippocampus, related to stimulation of D1-like and D2-like receptors and calcium influx.
Research Center of Biochemistry and Molecular Biology, Xuzhou Medical College, Xuzhou, Jiangsu 221002, China. xmcb@public.xz.js.cn
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Hou
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Zhang
D000818:Animals; D001058:Apomorphine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D004294:Domperidone / Q000494:pharmacology; D004298:Dopamine / Q000494:pharmacology*; D018491:Dopamine Agonists / Q000494:pharmacology; D018492:Dopamine Antagonists / Q000494:pharmacology; D006624:Hippocampus / Q000201:enzymology*; D066298:In Vitro Techniques; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D018491:Dopamine Agonists; D018492:Dopamine Antagonists; D004294:Domperidone; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D001058:Apomorphine; D002118:Calcium; D004298:Dopamine