Autophagy-related vacuoles in mouse gallbladder epithelium.
The mouse gallbladder epithelial cells contain very heterogeneous vacuolar population. In an attempt to classify these vacuoles we identified NADPase and TPPase activity as well as the location of HRP which is used as the endocytotic marker. The results of the present study show that the vacuoles can be classified into three categories: (1) the vacuoles predominantly containing loose membrane coils related to the nascent autophagic vacuoles, (2) vacuoles containing densely packed membranes and exhibiting a positive HRP reaction, indicating the convergence of endocytotic and autophagic pathway, and (3) vacuoles composed of degraded membrane structures and containing the reaction product of NADPase activity, showing that the fusion of the lysosomes with the autophagosome-endosome took place. The highly developed cis, medial and trans Golgi compartments reflect the biosynthetic and endocytotic activity of the gallbladder epithelium.
Institute of Cell Biology, Medical Faculty, University of Ljubljana, Slovenia. psenicnik@ibmi.mf.uni-lj.si
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D000818:Animals; D001343:Autophagy / Q000502:physiology*; D004705:Endocytosis / Q000502:physiology; D004847:Epithelial Cells / Q000648:ultrastructure*; D005089:Exocytosis / Q000502:physiology; D005260:Female; D005704:Gallbladder / Q000166:cytology*; D006056:Golgi Apparatus / Q000648:ultrastructure; D006735:Horseradish Peroxidase; D008247:Lysosomes / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D009708:Nucleotidases / Q000032:analysis; D013834:Thiamine Pyrophosphatase / Q000032:analysis; D014617:Vacuoles / Q000201:enzymology / Q000648:ultrastructure*
D006735:Horseradish Peroxidase; C021896:2'-nucleotidase; D009708:Nucleotidases; D013834:Thiamine Pyrophosphatase