Non-invasive approach for diagnosing atrophic gastritis using the 13C-bicarbonate breath test.
The experiments presented here were done to evaluate whether the levels of CO2 in respiratory air during the 13C-bicarbonate breath test (13C-BBT) may be used as a marker of non-invasive diagnosis of the levels of atrophic gastritis. Twenty-eight patients with chronic gastritis and five healthy volunteers were enrolled in the study. Moreover, experimental gastritis was induced in rats by N-methy-N-nitro-N-nitrosoguanidine. In human, the levels of atrophic gastritis were evaluated from the vascular pattern of the gastric fornix. Total delta 13CO2 calculated from the 13C-BBT and the mucosal thickness ratio (MTR) were measured in rats with experimental gastritis. The levels of 13CO2 were significantly higher from patients with a vascular pattern at the fornix than in those without a vascular pattern (p<0.01). There was a good correlation between MTR and the levels of 13CO2, in rats with experimental gastritis (p<0.01). These findings indicate that the levels of 13CO2 during 13C-BBT reflect the levels of atrophic gastritis and show its clinical significance for non-invasive evaluation of atrophic gastritis. This has important clinical implications in selecting Helicobacter pylori-positive cases for therapy and follow-up.
Kubo
Y
Y
Third Department of Internal Medicine, Ehime University School of Medicine, Shitsukawa, Shigenobu-cho, Onsen-gun, Ehime 791-0295, Japan. akbar@m.ehime-u.ac.jp
Matsui
H
H
Ninomiya
T
T
Mizukami
Y
Y
Onji
M
M
D000367:Age Factors; D000818:Animals; D001827:Body Height / Q000502:physiology; D001835:Body Weight / Q000502:physiology; D001944:Breath Tests / Q000379:methods; D002021:Buffers; D002245:Carbon Dioxide / Q000032:analysis; D002247:Carbon Isotopes; D004195:Disease Models, Animal; D005753:Gastric Mucosa / Q000187:drug effects / Q000473:pathology; D005757:Gastritis, Atrophic / Q000139:chemically induced / Q000175:diagnosis* / Q000473:pathology; D006801:Humans; D008297:Male; D008769:Methylnitronitrosoguanidine / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D012737:Sex Factors; D017693:Sodium Bicarbonate
D002021:Buffers; D002247:Carbon Isotopes; D008769:Methylnitronitrosoguanidine; D002245:Carbon Dioxide; D017693:Sodium Bicarbonate