Trimetazidine: stability indicating RPLC assay method.
An accurate, specific and reproducible reversed phase liquid chromatographic method for the determination of trimetazidine hydrochloride in presence of its degradation products is reported. The mobile phase consisted of water-acetonitrile-triethylamine (90:10:0.1, v/v/v) adjusted with o-phosphoric acid to a pH of 3.3. Chromatography was performed using C-18 column at a flow rate of 1.0 ml/min and the drug along with its degraded products was detected at 270 nm. The calibration curve of trimetazidine hydrochloride in methanol was linear in the range 500-3000 ng. The mean value of correlation coefficient, slope and intercept were 0.99859 &# 0.001, 17.7986 &# 0.0709 and 482.56 &# 147.03, respectively. The limits of detection and quantitation were 5 and 20 ng, respectively. The recovery of trimetazidine hydrochloride was about 99-100%. This method was utilized to analyze trimetazidine hydrochloride from conventional tablets and controlled release pellets in the presence of commonly used excipients.
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Department of Chemical Technology (Autonomous), Pharmaceutical Division, University of Mumbai, Nathalal Parikh Marg, Matunga, Mumbai 400 019, India.
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D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D015203:Reproducibility of Results; D012996:Solutions; D014292:Trimetazidine / Q000032:analysis*; D014665:Vasodilator Agents / Q000032:analysis*
D012996:Solutions; D014665:Vasodilator Agents; D014292:Trimetazidine