Induction of heme oxygenase 1 in liver of spontaneously diabetic rats.
It has been suggested that diabetes induces an increase in oxidative stress; the increased expression of heme-oxygenase 1 (HO-1) in liver is believed to be a sensitive marker of the stress response. The aim of this study was to examine whether diabetes is able to induce HO-1 expression in liver. The specific mRNA was amplified by RT/PCR and calibrated with amplified beta-actin mRNA. The mRNA HO-1 levels in the liver of spontaneously diabetic rats were increased by 1.8 fold compared with non diabetics; this supports the hypothesis of weak but significant oxidative damage due to chronic hyperglycaemia. This work represents the first in vivo study exploring the semi-quantitative expression of HO-1 in the liver of spontaneously diabetic rats.
Department of Experimental Medicine (DIMES), Section of General Pathology, University of Genova, Via L.B. Alberti 2, I-16132 Genoa, Italy. lcosso@hotmail.com
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Cosso
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Maineri
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Traverso
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Rosatto
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Pronzato
D
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Cottalasso
U M
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Marinari
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Odetti
D000818:Animals; D003922:Diabetes Mellitus, Type 1 / Q000201:enzymology*; D004195:Disease Models, Animal; D004790:Enzyme Induction; D006943:Hyperglycemia / Q000201:enzymology; D008099:Liver / Q000201:enzymology*; D008297:Male; D018384:Oxidative Stress; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011913:Rats, Inbred BB; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger