[Development and identification of T. aestivum-S. cereale-H. villosa double translocation line 1RS.1BL, 6VS.6AL via chromosome C-banding and dual color FISH].
In order to enhance the disease resistance of wheat-rye 1BL.1RS translocation line and broaden its genetic bases, a 1RS.1BL line was crossed to 6VS.6AL and from its F2 progenies, a double translocation line designated as 1RS.1BL, 6VS.6AL (2n = 42) was identified by (root tip cell) RTC chromosome C-banding. Its mean chromosome configuration at PMC MI was 19.14II + 1.86[symbol: see text] II indicating genetic stability. Rye and H. villosa genomic DNA labelled by biotin- and digoxingenin-11-dUTP respectively were used as probes simultaneously for dual color FISH identification. The results confirmed the C-banding results. Rye and H. villosa chromatin after FISH expressed green or red signals respectively in both RTC and PMC of the double translocation line. This line showed normal fertility, desirable agronomic traits and resistance to powdery mildew, and might be of interest for wheat improvement.
Qi
Z J
ZJ
Key Laboratory of Crop Cytogenetics of Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China.
Liu
D J
DJ
Chen
P D
PD
D002871:Chromosome Banding; D003433:Crosses, Genetic; D017404:In Situ Hybridization, Fluorescence; D010935:Plant Diseases / Q000235:genetics*; D012434:Secale / Q000235:genetics*; D014178:Translocation, Genetic; D014908:Triticum / Q000235:genetics*