Design, synthesis, and intracellular localization of a fluorescently labeled DNA binding polyamide related to the antibiotic distamycin.
The design and synthesis of the lipophilic (9) and fluorescent (10) conjugates of a structural analogue of distamycin and their in vitro cellular localization studies are reported. Confocal laser scanning microscopy (CLSM) indicates that 10 rapidly enters human ovarian adenocarcinoma (SKOV-3) cells with principal uptake in mitochondria and uniform cytoplasmic distribution.
Department of Chemistry, University of Alberta, Edmonton, Canada.
S K
SK
Sharma
A T
AT
Morrissey
G G
GG
Miller
W H
WH
Gmeiner
J W
JW
Lown
D000998:Antiviral Agents / Q000737:chemistry; D001692:Biological Transport; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D004214:Distamycins / Q000737:chemistry; D015195:Drug Design; D005260:Female; D005453:Fluorescence; D006801:Humans; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000378:metabolism; D009757:Nylons / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D010051:Ovarian Neoplasms; D014407:Tumor Cells, Cultured
D000998:Antiviral Agents; D004214:Distamycins; D009757:Nylons; C030000:stallimycin; D004247:DNA