Novel methods for biocatalyst screening.
There have been a number of recent advances in catalysis assays applicable for screening biocatalyst libraries in high-throughput format. These include instrumental assays such as high-performance liquid chromatography, mass spectrometry, capillary electrophoresis and IR-thermography, reagent-based assays producing spectroscopic signals (UV/VIS or fluorescence) in response to reaction progress, and assays based on fluorogenic or chromogenic substrates. These fluorogenic substrates enable the assaying of a variety of enzymes in enantioselective and stereoselective manner, including alcohol dehydrogenases, aldolases, lipases, amidases, epoxide hydrolases and phosphatases.
Wahler
D
D
Departement für Chemie und Biochemie, Universität Bern, Switzerland.
Reymond
J L
JL
D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002863:Chromogenic Compounds / Q000737:chemistry; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D004353:Drug Evaluation, Preclinical / Q000379:methods / Q000639:trends; D019075:Electrophoresis, Capillary / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004798:Enzymes / Q000235:genetics / Q000378:metabolism*; D005470:Fluorometry / Q000379:methods; D015723:Gene Library; D013058:Mass Spectrometry / Q000379:methods; D012641:Selection, Genetic; D013379:Substrate Specificity
D002352:Carrier Proteins; D002863:Chromogenic Compounds; D004798:Enzymes