Control of cystic fibrosis transmembrane conductance regulator expression by BAP31.
Expression of the cystic fibrosis transmembrane conductance regulator (CFTR) is stringently controlled by molecular chaperones participating in formation of the quality control system. It has been shown that about 75% of all CFTR protein and close to 100% of the [DeltaPhe(508)] CFTR variant are rapidly degraded before leaving the endoplasmic reticulum (ER). B cell antigen receptor-associated proteins (BAPs) are ubiquitously expressed integral membrane proteins that may control association with the cytoskeleton, vesicular transport, or retrograde transport from the cis Golgi to the ER. The present study delivers evidence for cytosolic co-localization of both BAP31 and CFTR and for the control of expression of recombinant CFTR in Chinese hamster ovary (CHO) cells and Xenopus oocytes by BAP31. Antisense inhibition of BAP31 in various cell types increased expression of both wild-type CFTR and [DeltaPhe(508)]CFTR and enabled cAMP-activated Cl(-) currents in [DeltaPhe(508)]CFTR-expressing CHO cells. Coexpression of CFTR together with BAP31 attenuated cAMP-activated Cl(-) currents in Xenopus oocytes. These data therefore suggest association of BAP31 with CFTR that may control maturation or trafficking of CFTR and thus expression in the plasma membrane.
Lambert
G
G
Physiologisches Institut, Universität Zürich Irchel, CH-8057 Zürich, Switzerland.
Becker
B
B
Schreiber
R
R
Boucherot
A
A
Reth
M
M
Kunzelmann
K
K
D000818:Animals; D016466:CHO Cells; D018118:Chloride Channels / Q000502:physiology; D002712:Chlorides / Q000378:metabolism; D006224:Cricetinae; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics*; D005786:Gene Expression Regulation / Q000502:physiology*; D017136:Ion Transport; D008565:Membrane Proteins; D011506:Proteins / Q000502:physiology*; D014981:Xenopus
D018118:Chloride Channels; D002712:Chlorides; D008565:Membrane Proteins; D011506:Proteins; D019005:Cystic Fibrosis Transmembrane Conductance Regulator