A transition metal enhanced luminol chemiluminescence in the presence of a chelator.
We have investigated the chemiluminescence signal of luminol and hydrogen peroxide in the presence of a transition metal (Co(II), Cu(I), Fe(II), Fe(III)) and of a chelator (EDTA, citric acid) in pH 8.5, 9 and 10 borate buffer solutions. We observed that the chemiluminescence intensities of these systems reached a plateau, where they remained stable for a period of 2-30 s. We also observed linearity between the intensity of chemiluminescence and the hydrogen peroxide concentration. The combination of Co(II) and EDTA at pH 9 was found to give the optimum signal with reference to time stability, intensity and reproducibility. Thus, compared to previous chemiluminescence applications, the present results permit us to propose a simple, enzyme-free and time-independent technique for the detection and quantification of hydrogen peroxide.
Parejo
I
I
Department of Natural Products, Faculty of Pharmacy, University of Barcelona, Avda. Diagonal 643, 08028, Catalonia, Barcelona, Spain.
Petrakis
C
C
Kefalas
P
P
D002021:Buffers; D002614:Chelating Agents / Q000737:chemistry*; D004492:Edetic Acid / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry*; D008670:Metals / Q000737:chemistry*; D012996:Solutions
D002021:Buffers; D002614:Chelating Agents; D007202:Indicators and Reagents; D008670:Metals; D012996:Solutions; D008165:Luminol; D004492:Edetic Acid; D006861:Hydrogen Peroxide