Garcinia extract inhibits lipid droplet accumulation without affecting adipose conversion in 3T3-L1 cells.
Garcinia extract was isolated from the fruit of the Garcinia cambogia and was used as a potential antiobesity agent. In week 3 of culture with insulin, the fat cells exhibited more numerous and larger intracytoplasmic lipid droplets (i.e. 30-40 microm(2)). When Garcinia extract and insulin were added simultaneously, the accumulation of lipid droplets was inhibited and the peak droplet area shifted to become smaller (10-20 microm(2)). The activities of glycerophosphate dehydrogenase, a marker of adipose differentiation, were not significantly inhibited by the Garcinia extract. These findings suggest that the Garcinia extract inhibits lipid droplet accumulation in fat cells without affecting adipose conversion.
Hasegawa
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Department of Food and Nutrition, Nagoya Bunri College, 2-1 Sasazuka-cho, Nishi-ku, Nagoya 451-0077, Japan. hsgwn@nagoya-bunri.ac.jp
D016475:3T3 Cells; D017667:Adipocytes / Q000187:drug effects*; D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology*; D050356:Lipid Metabolism; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology; D010946:Plants, Medicinal; D019685:Rosales
D019440:Anti-Obesity Agents; D010936:Plant Extracts