An experimental study evaluating the effect of Mitomycin C on the prevention of postoperative intraabdominal adhesions.
BACKGROUND
Fibroblast proliferation is one of the well-known mechanisms for postoperative intraabdominal adhesion formation. Inhibition of fibroblast proliferation is an attractive field of investigation in the prevention of adhesions. Mitomycin C (MMC) is a cytotoxic agent that alkylates and crosslinks DNA and also inhibits fibroblast proliferation up to a few weeks. We aimed to determine the effect of MMC on the prevention of adhesions.


METHODS
Generation of adhesions in rats by brushing a 1-cm(2) area of the cecum and the peritoneum on the right side of the abdominal wall was followed by intraperitoneal administration of saline, 1 mg/kg MMC, and 0.5 mg/kg MMC in saline. After 45 days, formation of adhesions was graded.


RESULTS
The average adhesion scores of the control, and MMC (1 mg/kg), MMC (0.5 mg/kg) groups were 3.2 +/- 0.7, 0.8 +/- 0.6, and 0.7 +/- 0.8, respectively. Adhesion scores of the two MMC-treated groups were significantly lower than that of the control group (P < 0.001). There was no difference between the two MMC groups (P > 0.05). No side effect of MMC was observed.


CONCLUSIONS
MMC was found to be very effective in the prevention of postoperative intraabdominal adhesions.
Department of General Surgery, Kocaeli University Medical School, Kocaeli, Turkey.
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Cubukçu
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Alponat
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Gönüllü
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Ozkan
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Erçin
D000818:Animals; D004341:Drug Evaluation; D005260:Female; D007410:Intestinal Diseases / Q000473:pathology / Q000517:prevention & control*; D016685:Mitomycin / Q000494:pharmacology*; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology*; D011183:Postoperative Complications / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar; D000267:Tissue Adhesions / Q000473:pathology / Q000517:prevention & control
D019384:Nucleic Acid Synthesis Inhibitors; D016685:Mitomycin