Altered gene expression in lymphocytes of patients with normal-tension glaucoma.
BACKGROUND
In glaucoma there is a loss of retinal ganglion cells. There is evidence that this loss can occur by apoptosis. The signal transduction leading to retinal ganglion cell apoptosis in glaucoma is not yet clear. The present study compares the gene expression in lymphocytes of normal tension glaucoma patients (NTG-patients) with the one of healthy controls.


METHODS
Subtractive hybridization was used to compare mRNA in lymphocytes of six vasospastic NTG-patients with six age and sex matched healthy subjects.


RESULTS
Genes coding for p53-protein, NTP (neural thread protein) and 20 S proteasome subunit XAPC7 were overexpressed, whereas those coding for XPGC (Xeroderma pigmentosum gene), the survivin protein as well as one type of ABC transport protein were underexpressed.


CONCLUSIONS
In comparison to healthy controls, patients with vasospastic NTG seem to over- as well as under-express certain genes in their lymphocytes.
Department of Ophthalmology, University of Basel, Basel, Switzerland.
O
O
Golubnitschaja-Labudova
R
R
Liu
C
C
Decker
P
P
Zhu
I O
IO
Haefliger
J
J
Flammer
D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D001483:Base Sequence; D003546:Cysteine Endopeptidases / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D004720:Endonucleases; D005136:Eye Proteins / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D005902:Glaucoma, Open-Angle / Q000378:metabolism*; D006801:Humans; D017403:In Situ Hybridization; D051034:Inhibitor of Apoptosis Proteins; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D008869:Microtubule-Associated Proteins; D008875:Middle Aged; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins / Q000235:genetics; D009687:Nuclear Proteins; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism*; D000077022:Survivin; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D015854:Up-Regulation; D000099250:DNA Excision Repair Protein ERCC-5
D018528:ATP-Binding Cassette Transporters; D003546:Cysteine Endopeptidases; D004268:DNA-Binding Proteins; D004720:Endonucleases; D005136:Eye Proteins; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D011506:Proteins; D012333:RNA, Messenger; D000077022:Survivin; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53; D000099250:DNA Excision Repair Protein ERCC-5; C109804:AD7c-NTP; C107489:BIRC5 protein, human