Molecular cloning, mapping and characterization of the human neurocalcin delta gene (NCALD).
We identified a new human gene that encodes a cognate of the bovine neurocalcin delta from a human fetal brain cDNA library; hence we named it human neurocalcin delta (NCALD) gene. The deduced polypeptide product of the cDNA is 22 kDa in size, and its amino acid sequence is 100% and 99% identical to that of the bovine and chicken neurocalcin, respectively. Northern blots showed that the NCALD gene is more abundantly expressed in brain, testis, ovary and small intestine. Tissue in situ hybridization confirmed the existence of the NCALD mRNA in the adult human testis. Radiation hybrid panel mapping localized the gene to chromosome 8 between molecular markers D8S270 and D8S257.
The Institute of Genetics, School of Life Science, Fudan University, 200433, Shanghai, PR China.
W
W
Wang
Z
Z
Zhou
W
W
Zhao
Y
Y
Huang
R
R
Tang
K
K
Ying
Y
Y
Xie
Y
Y
Mao
D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000235:genetics*; D002417:Cattle; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8; D003001:Cloning, Molecular; D018076:DNA, Complementary; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D051596:Neurocalcin; D016366:Open Reading Frames; D044169:Receptors, Calcium-Sensing; D013737:Testis / Q000378:metabolism / Q000473:pathology; D014018:Tissue Distribution
D002135:Calcium-Binding Proteins; D018076:DNA, Complementary; C496685:NCALD protein, human; D009419:Nerve Tissue Proteins; D051596:Neurocalcin; D044169:Receptors, Calcium-Sensing