A simple, rapid, and highly efficient gene expression system for multiheme cytochromes c.
The genes of tetraheme cytochrome c3 and hexadecaheme high-molecular-weight cytochrome c from Desulfovibrio vulgaris could be overexpressed as holoproteins in Shewanella oneidensis TSP-C using pUC-type vectors of E. coli. Surprisingly, S. oneidensis was transformed directly by pUC-type vectors through electroporation. The yields of the recombinant proteins in this expression system were much higher than the previously reported ones.
Department of Chemistry and Biotechnology, Faculty of Engineering, Yokohama National University, Japan.
K
K
Ozawa
F
F
Yasukawa
Y
Y
Fujiwara
H
H
Akutsu
D003574:Cytochrome c Group / Q000235:genetics* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D009682:Magnetic Resonance Spectroscopy; D010084:Oxidation-Reduction; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D020592:Shewanella / Q000235:genetics* / Q000378:metabolism
D003574:Cytochrome c Group; D011994:Recombinant Proteins