Apolipoprotein E4 stimulates sulfation of glycosaminoglycans in neural cells.
Apolipoprotein E externally added to neuroblastoma cells in culture stimulates [35S]sulfate incorporation on cell and extracellular matrix glycosaminoglycans (sGAG). This stimulation is mainly observed for ApoE4 compared to ApoE3. The increase in sulfation is not due to increased synthesis as there is no corresponding increase in the [3H]glucosamine incorporation. Since the presence of ApoE is a risk factor for Alzheimer's disease (AD) and the presence of sGAG could facilitate the assembly of the main components, beta-amyloid and tau proteins, of the aberrant structures found in AD, the present study indicates a possible relation between those factors.
Centro de Biología Molecular 'Severo Ochoa', Universidad Autónoma de Madrid, 28049, Madrid, Spain. pbonay@cbm.uam.es
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P
Bonay
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J
Avila
D000544:Alzheimer Disease / Q000378:metabolism*; D053327:Apolipoprotein E4; D001057:Apolipoproteins E / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D005944:Glucosamine / Q000737:chemistry; D006025:Glycosaminoglycans / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D012307:Risk Factors; D013431:Sulfates / Q000737:chemistry; D013462:Sulfur Radioisotopes; D014316:Tritium; D014407:Tumor Cells, Cultured
D053327:Apolipoprotein E4; D001057:Apolipoproteins E; D006025:Glycosaminoglycans; D013431:Sulfates; D013462:Sulfur Radioisotopes; D014316:Tritium; D005944:Glucosamine