Morphologic evaluation and integrin expression profile of renal tubular cells cultured from percutaneous renal biopsy specimen.
Kidney biopsy is an indispensible procedure for making a pathologic diagnosis of renal diseases by fixing and staining the biopsy specimen. However, it is not a routine procedure to culture the cells from a renal biopsy specimen directly, or to utilize the cultured cells for any kind of diagnostic or functional evaluation. In this study, primary culture of the renal tubular epithelial cells was tried from a piece of percutaneous kidney biopsy specimen. Successive passages of the cells were possible until fourth passage. With these cells, morphologic characteristics of the cultured cells and integrin expression profiles were investigated. On light and electron microscopy, these cells were characterized by the cobblestone-like growth, presence of microvilli and tight junction, and the preservation of polarity. Immunohistochemical studies demonstrated the epithelial nature of these cells and particularly their differentiation from renal tubular epithelial cells, of either proximal or distal nephronic segment. The integrin profile confirms the epithelial nature of the cell. We hope that our results facilitate the understanding of pathophysiology of renal tubular cells from the patient directly.
Department of Pediatrics, School of Medicine, Samsung Medical Center, Samsung Biomedical Research Center, Seoul, Korea.
J S
JS
Kim
H Z
HZ
Hwang
S H
SH
Yeo
S W
SW
Ko
S M
SM
Song
Y K
YK
Kim
D J
DJ
Kim
H Y
HY
Oh
H Y
HY
Choi
M K
MK
Kim
S
S
Toru
T
T
Kohsaka
Y
Y
Kim
D K
DK
Jin
D001706:Biopsy; D015152:Blotting, Northern; D002455:Cell Division; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology; D005434:Flow Cytometry; D006801:Humans; D016023:Integrins / Q000096:biosynthesis*; D007668:Kidney / Q000473:pathology; D007684:Kidney Tubules / Q000166:cytology* / Q000378:metabolism; D008854:Microscopy, Electron
D016023:Integrins