DnaA protein dependent denaturation of negative supercoiled oriC DNA minicircles.
The DnaA protein binds specifically and tightly to oriC supercoiled 641 bp minicircle DNA. The binding of the initiator promoted a partial unwinding of the superhelical oriC minicircle (Mc-oriC). Open complexes are detected either by a change in the linking number or by the sensitivity to the attack of a single strand specific Bal 31 nuclease. The open complex is found only in the presence of the DnaA protein.
Département de Biologie, Laboratoire de Biochimie des Proteines, Faculté des Sciences de Tunis, Campus Universitaire, 1060, Tunis, Tunisia.
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Landoulsi
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Kohiyama
D000255:Adenosine Triphosphate / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004264:DNA Topoisomerases, Type I / Q000235:genetics / Q000378:metabolism*; D004278:DNA, Superhelical / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D018741:Replication Origin / Q000235:genetics*
D001426:Bacterial Proteins; D004278:DNA, Superhelical; D004268:DNA-Binding Proteins; C066312:DnaA protein, Bacteria; D000255:Adenosine Triphosphate; D004264:DNA Topoisomerases, Type I