Modulation of P-CREB and expression of c-fos in cochlear nucleus and superior olive following electrical intracochlear stimulation.
Investigating activity-dependent plasticity in the auditory brain stem of the adult rat, we observed that electrical intracochlear stimulation led to a tonotopically localized modulation of the phosphorylation of the cAMP response element binding protein (CREB) and an equally localized expression of the immediate early gene product c-Fos in cochlear nucleus and superior olive. As P-CREB is thought to act as transcription factor on one promoter site of the c-fos gene, we compared immunolabeling for P-CREB and c-fos in adjacent brain sections. Following 2h sustained stimulation in previously deafened animals, labelling for P-CREB declined in regions where c-Fos labelling increased. This suggests that the level or state of P-CREB (e.g. whether it is phosphorylated or not) are affected by intracochlear stimulation in a process that appears to be linked to the stimulation-dependent expression of c-Fos in auditory brain stem nuclei.
Department of Otorhinolaryngology, University of Freiburg, Germany.
R B
RB
Illing
S A
SA
Michler
D000818:Animals; D000906:Antibodies; D003054:Cochlear Implants; D017626:Cochlear Nucleus / Q000502:physiology*; D017362:Cyclic AMP Response Element-Binding Protein / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D004558:Electric Stimulation; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D009473:Neuronal Plasticity / Q000502:physiology; D009847:Olivary Nucleus / Q000502:physiology*; D010766:Phosphorylation; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar
D000906:Antibodies; D017362:Cyclic AMP Response Element-Binding Protein; D016760:Proto-Oncogene Proteins c-fos