Fate of mat1 DNA strands during mating-type switching in fission yeast.
The mating-type switching of the fission yeast, Schizosaccharomyces pombe, is highly regulated. Two consecutive asymmetric divisions are required to produce one mating-type switched cell among the four progeny. Using DNA density-gradient centrifugation we demonstrate that one-fourth of the mat1 DNA is not replicated by the conventional semi-conservative mode, but instead both DNA strands are synthesized de novo. Our data are consistent with a gene conversion event, initiated by a site- and strand-specific DNA break (SSB). We further demonstrate that the virgin switched mat1-containing chromatid no longer contained the nick, while it is reintroduced during the lagging strand synthesis of the mat1 locus on the sister chromatid. This finding establishes at the molecular level a firm experimental link between the phenotype and genotype in the process of asymmetric mating-type switching during mitotic divisions.
Arcangioli
B
B
Unité des Virus Oncogènes, URA 1644 du CNRS, Département des Biotechnologies, Institut Pasteur, Paris, France. barcan@pasteur.fr
D015139:Blotting, Southern; D002499:Centrifugation, Density Gradient; D004261:DNA Replication; D004271:DNA, Fungal / Q000096:biosynthesis*; D005785:Gene Conversion; D005800:Genes, Fungal; D049770:Genes, Mating Type, Fungal; D005838:Genotype; D008938:Mitosis / Q000502:physiology*; D009693:Nucleic Acid Hybridization; D010375:Pedigree; D010641:Phenotype; D012568:Schizosaccharomyces / Q000235:genetics* / Q000502:physiology
D004271:DNA, Fungal