Expression of Semliki Forest virus E1 protein in Escherichia coli. Low pH-induced pore formation.
Exposure of Semliki Forest virus 1 to mildly acidic conditions results in conformational changes of the viral spike proteins, which in turn leads to a pore formation across its membrane. The ability to form a pore has been ascribed to the ectodomain of the Semliki Forest virus (SFV) E1 spike protein. To elucidate whether the E1 protein per se is sufficient for low pH-dependent pore formation, we expressed E1 in Escherichia coli in an inducible manner using the pET11c expression system. The data obtained clearly showed that the E1 protein was expressed in the bacterial cell membrane and that exposure of E. coli expressing the SFV E1 protein to low pH (<6.2) resulted in a permeability change of the membrane. Thus, we conclude that the E1 protein of SFV per se is sufficient to promote pore formation under mildly acidic conditions.
Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, 3012 Bern, Switzerland.
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Nyfeler
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Senn
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Kempf
D002463:Cell Membrane Permeability; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics* / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D010957:Plasmids; D012672:Semliki forest virus / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
D014764:Viral Proteins