CD 68 positivity of the so-called meconium corpuscles in human foetal intestine.
CD 68 antibodies are most frequently used as a reliable paraffin marker of histiocytes. The CD 68 clusters of antibodies recognize a 110-kDa glycoprotein associated with lysosomes and therefore these antibodies show visible reactivity with cells of the monocyte/macrophage lineage. We observed distinct CD 68 positivity of granular intraepithelial inclusions in the small intestine of 12-week-old human foetuses. These PAS positive inclusions corresponded to the so-called meconium particles, whose lysosomal origin was confirmed by electron microscopy. Our new observation shows that CD 68 antibodies are specific mainly for organelles and not for a cell type and therefore the staining of epithelial cells containing lysosomes is possible.
Ustav patologie 1. LF UKa VFN, Katedra patologické anatomie IPVZ, Praha.
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D015703:Antigens, CD / Q000032:analysis*; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis*; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D005333:Fetus / Q000276:immunology / Q000648:ultrastructure*; D006801:Humans; D007150:Immunohistochemistry; D002479:Inclusion Bodies / Q000276:immunology* / Q000648:ultrastructure; D007413:Intestinal Mucosa / Q000648:ultrastructure*; D008247:Lysosomes / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D008470:Meconium; D000097382:CD68 Molecule
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; C067980:CD68 antigen, human; D000097382:CD68 Molecule