Cloning and expression of novel mosaic serine proteases with and without a transmembrane domain from human lung.
Two cDNAs encoding novel mosaic proteins with a serine protease domain and potential regulatory modules, consisting of a protein kinase substrate and a low-density lipoprotein receptor, were cloned from a human lung cDNA library by PCR. One with a transmembrane domain (MSPL) and the other without one (MSPS) comprise 581 and 537 amino acids, respectively. Except for the C-terminal ends, the two isoforms had an identical serine protease domain exhibiting 42, 39 and 43% identity with those of plasma kallikrein, hepsin and transmembrane protease serine 2, respectively. Both genes were predominantly expressed in human lung, placenta, pancreas and prostate.
Kim
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Division of Enzyme Chemistry, Institute for Enzyme Research, The University of Tokushima, 770-8503, Tokushima, Japan.
Sharmin
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Inoue
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Kido
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H
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D002462:Cell Membrane / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015870:Gene Expression; D006801:Humans; D008168:Lung / Q000201:enzymology; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D011973:Receptors, LDL / Q000235:genetics*; D012697:Serine Endopeptidases / Q000235:genetics* / Q000378:metabolism; D014018:Tissue Distribution; D014357:Trypsin / Q000235:genetics*
D018076:DNA, Complementary; D008565:Membrane Proteins; D011973:Receptors, LDL; D012697:Serine Endopeptidases; C429035:TMPRSS13 protein, human; D014357:Trypsin