Cloning and expression of novel mosaic serine proteases with and without a transmembrane domain from human lung.
Two cDNAs encoding novel mosaic proteins with a serine protease domain and potential regulatory modules, consisting of a protein kinase substrate and a low-density lipoprotein receptor, were cloned from a human lung cDNA library by PCR. One with a transmembrane domain (MSPL) and the other without one (MSPS) comprise 581 and 537 amino acids, respectively. Except for the C-terminal ends, the two isoforms had an identical serine protease domain exhibiting 42, 39 and 43% identity with those of plasma kallikrein, hepsin and transmembrane protease serine 2, respectively. Both genes were predominantly expressed in human lung, placenta, pancreas and prostate.
Division of Enzyme Chemistry, Institute for Enzyme Research, The University of Tokushima, 770-8503, Tokushima, Japan.
D R
DR
Kim
S
S
Sharmin
M
M
Inoue
H
H
Kido
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D002462:Cell Membrane / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015870:Gene Expression; D006801:Humans; D008168:Lung / Q000201:enzymology; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D011973:Receptors, LDL / Q000235:genetics*; D012697:Serine Endopeptidases / Q000235:genetics* / Q000378:metabolism; D014018:Tissue Distribution; D014357:Trypsin / Q000235:genetics*
D018076:DNA, Complementary; D008565:Membrane Proteins; D011973:Receptors, LDL; D012697:Serine Endopeptidases; C429035:TMPRSS13 protein, human; D014357:Trypsin