Accumulation of LOX-1 ligand in plasma and atherosclerotic lesions of Watanabe heritable hyperlipidemic rabbits: identification by a novel enzyme immunoassay.
LOX-1 (lectin-like oxidized LDL receptor) is a newly identified cell surface receptor for oxidized LDL mainly expressed in endothelial cells. Recombinant soluble LOX-1(LOX-Fc) was generated by fusing the extracellular domain of LOX-1 with the Fc portion of IgG. A novel sandwich enzyme immunoassay specific for LOX-1 ligand is designed, using LOX-Fc and anti-apoB antibody. This immunoassay was used to determine LOX-1 ligand activity in normal and Watanabe heritable hyperlipidemic (WHHL) rabbit plasma. LOX-Fc was further applied for staining of atherosclerotic lesions of WHHL rabbits. LOX-1 ligand levels were significantly elevated in the plasma of hyperlipidemic rabbits compared with controls. Furthermore, LOX-1 ligand activity was detected in the atherosclerotic lesions in situ. These results support the potential roles of LOX-1 interacting with its ligand in the pathogenesis of atherosclerosis, which is enhanced in hyperlipidemia.
Kakutani
M
M
Department of Bioscience, National Cardiovascular Center Research Institute, Suita, 565-8565, Japan.
Ueda
M
M
Naruko
T
T
Masaki
T
T
Sawamura
T
T
D000818:Animals; D001161:Arteriosclerosis / Q000378:metabolism*; D020022:Genetic Predisposition to Disease; D006949:Hyperlipidemias / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D007124:Immunoenzyme Techniques / Q000379:methods*; D007150:Immunohistochemistry; D008024:Ligands; D008077:Lipoproteins, LDL / Q000097:blood / Q000378:metabolism*; D011817:Rabbits; D011973:Receptors, LDL / Q000378:metabolism*; D051131:Receptors, Oxidized LDL
D008024:Ligands; D008077:Lipoproteins, LDL; D011973:Receptors, LDL; D051131:Receptors, Oxidized LDL; C099252:oxidized low density lipoprotein