Design of a unique PCR primer for detection of oral HPV infection.
Human papillomavirus (HPV) has been known as a pathogen of oral dysplasia. However, suitable PCR primers for the detection of oral HPV infection have not been reported. The aim of this study was to design unique consensus primers. The consensus primers were designed by homologues analyses between subtypes 2, 6, 11, 13, 16, 18, 30, 32 and 58, which frequently infect the oral membrane. PCR, with our designed primer, detected HPV DNA subtypes 2, 6, 11, 16, 18 and 58, and also showed PCR product from a clinical papilloma sample. These results indicate that our designed consensus primer can be used for the study of the relationships between oral disease and HPV infection.
Department of Comprehensive Dentistry, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
T
T
Kuroki
O
O
Tsuzukibashi
M
M
Saito
S
S
Kuribayashi
Y
Y
Shimpuku
M
M
Makimura
D016384:Consensus Sequence; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D015151:Immunoblotting; D009059:Mouth Diseases / Q000175:diagnosis* / Q000821:virology; D009061:Mouth Mucosa / Q000821:virology; D030361:Papillomavirus Infections / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000379:methods
D015344:DNA Probes, HPV; D004279:DNA, Viral