Grabbing the cat by the tail: manipulating molecules one by one.
Methods for manipulating single molecules are yielding new information about both the forces that hold biomolecules together and the mechanics of molecular motors. We describe here the physical principles behind these methods, and discuss their capabilities and current limitations.
Howard Hughes Medical Institute, Berkeley, California, USA. carlos@alice.berkeley.edu
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Bustamante
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Macosko
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Wuite
D004247:DNA / Q000737:chemistry / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000378:metabolism; D008832:Microchemistry / Q000379:methods; D018625:Microscopy, Atomic Force / Q000379:methods; D008958:Models, Molecular; D008967:Molecular Biology / Q000379:methods*; D020409:Molecular Motor Proteins / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure; D011506:Proteins / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure
D020409:Molecular Motor Proteins; D011506:Proteins; D004247:DNA; D012321:DNA-Directed RNA Polymerases