[Use of toxic fraction isolated from Algerian Androctonus australis hector scorpion venom for the assessment of anti-venom serum].
Today, serotherapy is the only specific treatment used against the scorpionique envenomation. This treatment was associated with other drugs as a symptomatic and supportive care. The anti-venom against scorpion stings is still prepared using the conventional manufacturing production. To evaluate the efficacy of the anti-venom, different protocols are used: The immunization of animals (rabbit and horse) with a whole toxic fraction prepared by molecular filtration was the first step to improve the anti-venom. Some observations also show the need for the quality control of this preparation of anti-venom. In this study, we describe the use the toxic fraction isolated from Androctonus australis Hector in the new protocol of anti-venom production. The quality control of the product is evaluated by using a combination of experimental immunological assay systems (ELISA, Hemaglutination, in vivo assessment of the neutralization of the lethal effect of the venom).
Laboratoire de Recherche et Développement sur les venins, Institut Pasteur d'Algérie.
F
F
Laraba-Djebari
D
D
Hammoudi
D000462:Algeria; D000818:Animals; D000997:Antivenins / Q000276:immunology*; D012604:Scorpion Venoms / Q000276:immunology*; D013045:Species Specificity
D000997:Antivenins; D012604:Scorpion Venoms