Differences between predicted and observed sequences in Saccharomyces cerevisiae.
We recently studied the protein composition of a Saccharomyces cerevisiae wine yeast strain (K310) of enological interest. About 2,500 spots of 8-250 kDa observed molecular mass were resolved by two-dimensional gel electrophoresis. Experimental molecular masses and isoelectric points were calculated for most of them. Twenty-seven proteins were subjected to Edman microsequencing. N-terminal sequences of 12/27 proteins were determined, whereas internal sequences of 6/27 proteins were obtained following in situ proteolysis. Comparison between the experimental data and those reported in the SWISS-PROT database revealed some differences between genotypic and phenotypic sequences. These are indicative of the changes a protein can undergo with respect to the primary structure coded by the genomic DNA. Our results highlight the need to complement genomic analysis with detailed proteomics in order to refine the vast amount of information provided by DNA sequencing and to find an exact correlation between genome and proteome.
Santucci
A
A
Dipartimento di Biologia Molecolare, Università degli Studi di Siena, Italy. santucci@unisi.it
Trabalzini
L
L
Bovalini
L
L
Ferro
E
E
Neri
P
P
Martelli
P
P
D016208:Databases, Factual; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005656:Fungal Proteins / Q000032:analysis*; D012441:Saccharomyces cerevisiae / Q000737:chemistry*
D005656:Fungal Proteins