Cell culture and animal studies for intracerebral delivery of borocaptate in liposomal formulation.
The efficacy of a liposomal formulation for intracerebral delivery of borocaptate (BSH) to brain tumor cells has been investigated using cell culture to study BSH uptake and persistence and using tumor-bearing rats to determine BSH distribution in the brain. During a 16-hr incubation, cellular uptake of BSH solution or BSH liposomal formulation was similar. However, the cellular persistence of BSH greatly increased when BSH was present in liposome. The differences in cellular persistence for BSH solution and BSH-loaded liposomes were significant both in 12-hr and 24-hr incubation experiments (p < 0.05 and p < 0.01, respectively). For the studies involving tumor-bearing rats, BSH level in tumor tissue was significantly higher than that in normal brain tissue at 2 hr and 6 hr after intracerebral injection of BSH-loaded liposomes (p < 0.01). Our study indicated that the liposomal formulation enhanced cellular persistence of BSH in tumor cells and therefore favored the boron accumulation in the cells. With the prolonged physical retention of liposomes at the local injection site and the cellular retention of BSH enhanced by the liposomes, the intracerebral delivery of BSH using liposomal formulation may provide an effective boron delivery approach for boron neutron capture therapy of brain tumors.
College of Pharmacy, University of Georgia, Athens 30602, USA.
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Chen
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Halpern
D000818:Animals; D001894:Borohydrides / Q000493:pharmacokinetics* / Q000494:pharmacology; D016754:Boron Neutron Capture Therapy; D001921:Brain / Q000378:metabolism; D001932:Brain Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D016503:Drug Delivery Systems; D005910:Glioma / Q000188:drug therapy / Q000378:metabolism*; D007267:Injections; D007276:Injections, Intraventricular; D008081:Liposomes; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D013438:Sulfhydryl Compounds / Q000493:pharmacokinetics* / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects
D001894:Borohydrides; D008081:Liposomes; D013438:Sulfhydryl Compounds; C014651:mercaptoundecahydrododecaborate