Genomic tools for gene and protein discovery in malaria: toward new vaccines.
Advances in malaria vaccine and drug development have been hindered in part by the complex multistage life cycle of the parasite, much of which is inaccessible to study, and by a large genome encoding over 5000 genes. Two human models of immunity to malaria, however, suggest that the development of an effective vaccine is within reach. We have outlined a strategy to identify the expression of hundreds to thousands of potential vaccine targets employing recently developed technologies for gene and protein expression. Combined with the exciting developments of malaria DNA vaccine technologies, these approaches form the basis for malaria subunit vaccines that may mimic the protective efficacy of our human model systems and provide the foundation for novel approaches to vaccine development for a range of pathogens.
Carucci
D J
DJ
Malaria Program, Naval Medical Research Center, 20910, Silver Spring, MD, USA
D000818:Animals; D016208:Databases, Factual; D017125:Genes, Protozoan; D006801:Humans; D008288:Malaria / Q000276:immunology / Q000517:prevention & control; D017780:Malaria Vaccines / Q000235:genetics* / Q000302:isolation & purification*; D008954:Models, Biological; D020411:Oligonucleotide Array Sequence Analysis; D010961:Plasmodium / Q000235:genetics* / Q000276:immunology*; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology; D019444:Vaccines, DNA / Q000235:genetics / Q000302:isolation & purification
D017780:Malaria Vaccines; D015800:Protozoan Proteins; D019444:Vaccines, DNA