[Analyses of population genetic structure by using dominant markers].
Dominant markers tend to under-estimate the amount of genetic diversity relative to codominant systems when applying in population genetics. In order to compare various existing methods for analysis of genetic structure, RAPD markers were used to detect genetic variability of 5 populations of Oryza granulata from China. The results indicated that both Shannon index of diversity and Nei gene diversity were superior to percentage of polymorphic bands (PPB) because the latter lacked of ability to describe frequency difference of polymorphic bands. Mantel test showed significant relation (r > 0.95, t > t0.01) among matrice of 17 different genetic similarities, which indicated that all of them could be used in analysis of genetic relations of individuals of Oryza granulata. Both AMOVA analysis based on phi st distance and analysis of Nei's distance showed consistent results in defining relationship among the 5 populations, and Lynch-Milligan pruning should be used to improve the estimation of population parameters. All of AMOVA, Gst and Shannon diversity analyses obtained similar results with majority of genetic variation occurring between Yunnan and Hainan, and low levels of genetic diversity resided within regions and populations.
Laboratory of Systematic and Evolutionary Botany, Chinese Academy of Sciences, Beijing 100093, China.
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D005819:Genetic Markers; D014644:Genetic Variation; D012275:Oryza / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique
D005819:Genetic Markers