Location of the O-methyl groups in the O polysaccharide of Pseudomonas syringae pv. phaseolicola.
The O-methylation pattern of the O polysaccharide (OPS) of the lipopolysaccharide of Pseudomonas syringae pv. phaseolicola GSPB 1552 was revealed by methylation (CD3I) analysis, Smith degradation, and NMR spectroscopy. Together with the major O repeats consisting of D-rhamnopyranose (D-Rhap) and D-fucofuranose (D-Fucf), there are minor repeats (approximately 30%) containing 3-O-methyl-D-rhamnose (D-acofriose), which is 2-substituted in the interior repeats and occupies the terminal non-reducing end of the OPS. It was suggested that the methylated O repeats are linked to each other nearby the non-reducing end of the OPS and that the 'biological' O repeat of the OPS has the following structure: [molecular structure: see text].
Zdorovenko
E L
EL
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow.
Ovod
V V
VV
Zatonsky
G V
GV
Shashkov
A S
AS
Kocharova
N A
NA
Knirel
Y A
YA
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D008358:Mannose / Q000031:analogs & derivatives* / Q000737:chemistry; D008745:Methylation; D008969:Molecular Sequence Data; D008970:Molecular Weight; D019906:Nuclear Magnetic Resonance, Biomolecular; D019081:O Antigens / Q000737:chemistry*; D011549:Pseudomonas / Q000737:chemistry*; D012210:Rhamnose / Q000737:chemistry
D019081:O Antigens; C095976:O antigen, Pseudomonas; C006396:acofriose; D008358:Mannose; D012210:Rhamnose