Laboratory methods in ophthalmic genetics: obtaining DNA from patients.
DNA samples are the fundamental research substrate in genetics. Although methodology and cost-effectiveness in molecular biology have improved dramatically, collecting biological samples and extracting DNA continue to be expensive and time-consuming steps of genetic research. This article reviews the issues surrounding the choice of biological samples for methods of DNA extraction as well as the storage and transport of biological and DNA samples for genetic studies.
Dickinson
J L
JL
Menzies Centre for Population Health Research, University of Tasmania, Hobart, Tasmania, Australia.
Sale
M M
MM
Craig
J E
JE
Mackey
D A
DA
D004247:DNA / Q000302:isolation & purification*; D005128:Eye Diseases / Q000235:genetics* / Q000473:pathology; D007258:Informed Consent; D008967:Molecular Biology / Q000191:economics / Q000379:methods*; D009885:Ophthalmology / Q000191:economics / Q000379:methods*; D013048:Specimen Handling
D004247:DNA