Determination of 24(R)-pseudoginsenoside F(11) in North American ginseng using high performance liquid chromatography with evaporative light scattering detection.
A gradient liquid chromatographic method with evaporative light scattering detection (ELSD) for the determination of 24(R)-pseudoginsenoside F(11) in North American ginseng is described. Samples are analyzed by means of a reverse-phase column (Waters Spherisorb ODS-2, C(18)) using acetonitrile and water under gradient conditions as the mobile phase over 20 min. The evaporative light scattering detector (ELSD) used, was set at an evaporating temperature of 35 degrees C and nitrogen gas pressure of 3.4 bar. The detection limit (S/N>5) of 24(R)-pseudoginsenoside F(11) is 53 ng on column.
Functional Foods for Health (FFH) Core Analytical Laboratory, Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, 833 South Wood Street, Chicago, IL 60612, USA.
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Li
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Fitzloff
D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D036145:Ginsenosides; D007202:Indicators and Reagents; D008027:Light; D005894:Panax / Q000737:chemistry*; D010946:Plants, Medicinal; D012015:Reference Standards; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis*; D012542:Scattering, Radiation; D013056:Spectrophotometry, Ultraviolet
D036145:Ginsenosides; D007202:Indicators and Reagents; D012503:Saponins; C413985:pseudoginsenoside F11