Lactobacillus plantarum phytase activity is due to non-specific acid phosphatase.
Microbial phytases suitable for food fermentations could be obtained from lactic acid bacteria isolated from natural vegetable fermentations. Phytase activity was evaluated for six lactic acid bacteria cultures. Although the highest activity was found for Lactobacillus plantarum, the phytase activity was very low. Further characterization of the enzyme with phytate-degrading activity showed a molecular weight of 52 kDa and an optimum activity at pH 5.5 and 65 degrees C. Enzyme activity was due to a non-specific acid phosphatase which had a higher hydrolysis rate with monophosphorylated compounds such as acetyl phosphate that could explain the low phytase activity.
Facultad de Ingeniería Química, Universidad Autónoma de Yucatán, Mérida, Yucatán, México. zmaya@tunku.uady.mx
M
M
Zamudio
A
A
González
J A
JA
Medina
D010832:6-Phytase / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D000135:Acid Phosphatase / Q000378:metabolism*; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus / Q000201:enzymology*; D008970:Molecular Weight; D013379:Substrate Specificity; D013696:Temperature
D000135:Acid Phosphatase; D010832:6-Phytase