The use of flow cytometry as a tool for monitoring filament formation of fungi.
Flow cytometry (FC) has the ability to discriminate a variety of cell parameters including cell size and complexity, and fluorescence intensity. As yeast cells or fungal spores germinate they undergo a morphological transformation from round oval shaped cells to elongate filamentous forms. To date, monitoring these events has been performed using microscopic examination. Microscopic examination is a labor intensive process that examines a very small percentage of the total cell population. We have developed a method using FC that is rapid, simple to perform, and reproducible. The major advantages of FC include analysis of a larger number of cells, increased objectivity due to nonselective measurements of all cells in the population studied, and the computer related data analysis capability of the flow cytometer.
Clinical Microbiology and Immunology Laboratories UNC Hospitals, Chapel Hill, NC 27514, USA. rhopfer@unch.unc.edu
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D002175:Candida / Q000254:growth & development*; D005434:Flow Cytometry / Q000379:methods; D015203:Reproducibility of Results; D013194:Staining and Labeling