Protein analysis of an individual Caenorhabditis elegans single-cell embryo by capillary electrophoresis.
We present a simple one-dimensional electrophoretic map of the expressed proteins in a Caenorhabditis elegans embryo. The embryo was taken from an adult nematode, injected into a 50-microm I.D. capillary, and lysed. The proteins were fluorescently labeled and then separated by capillary electrophoresis and detected by laser-induced fluorescence. Over 20 components were resolved in the 22-min separation. The dynamic range was outstanding for this separation, noise in the baseline was less than 0.01% the amplitude of the largest component.
Department of Chemistry, University of Alberta, Edmonton, Canada.
S
S
Hu
R
R
Lee
Z
Z
Zhang
S N
SN
Krylov
N J
NJ
Dovichi
D000818:Animals; D017173:Caenorhabditis elegans / Q000196:embryology*; D019075:Electrophoresis, Capillary / Q000379:methods*; D004625:Embryo, Nonmammalian / Q000737:chemistry*; D005453:Fluorescence; D015801:Helminth Proteins / Q000032:analysis*
D015801:Helminth Proteins