MR microscopy of cobalt-labeled nerve cells and pathways in an invertebrate brain (Sepia officinalis, Cephalopoda).
This article describes a novel application of contrast-enhanced MR microscopy to trace nerve cells and pathways through small invertebrate brains. Using the cuttlefish Sepia officinalis (Cephalopoda) as a model, the cells and pathways of one of the brain nerves were labeled with paramagnetic cobalt(II) ions by conventional centripetal cobalt iontophoresis. In MR microscopy, the cobalt-labeled cell bodies and pathways became hypointense in 9.4 T spin echo images. Their course and distribution were identical with those seen with conventional histological techniques after cobalt sulphide precipitation (with or without subsequent silver intensification). Magn Reson Med 45:575-579, 2001.
Quast
M J
MJ
Marine Biomedical Institute, University of Texas Medical Branch, Galveston, Texas, USA. mjquast@utmb.edu
Neumeister
H
H
Ezell
E L
EL
Budelmann
B U
BU
D000818:Animals; D001921:Brain / Q000166:cytology; D003035:Cobalt; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D023421:Models, Animal; D008974:Mollusca; D009434:Neural Pathways / Q000166:cytology; D009474:Neurons / Q000166:cytology*
D003035:Cobalt