Sequence analysis and overexpression of a pectin lyase gene (pel1) from Aspergillus oryzae KBN616.
A gene (pel1) encoding pectin lyase (Pel1) was isolated from a shoyu koji mold, Aspergillus oryzae KBN616, and characterized. The structural gene comprised 1,196 bp with a single intron. The ORF encoded 381 amino acids with a signal peptide of 20 amino acids. The deduced amino acid sequence showed high similarity to those of Aspergillus niger pectin lyases and Glomerella cingulata PnlA. The pel1 gene was successfully overexpressed under the promoter of the A. oryzae TEF1 gene. The molecular mass of the recombinant pectin lyase substantially coincided with that calculated based on nucleotide sequence.
Food Research Institute, Aichi Prefectural Government, Nagoya, Japan. kn-afri@aichi-iic.or.jp
N
N
Kitamoto
S
S
Yoshino-Yasuda
K
K
Ohmiya
N
N
Tsukagoshi
D000595:Amino Acid Sequence; D001236:Aspergillus oryzae / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D005800:Genes, Fungal / Q000235:genetics*; D008969:Molecular Sequence Data; D011133:Polysaccharide-Lyases / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D017422:Sequence Analysis, DNA
D011133:Polysaccharide-Lyases; C027349:pectin lyase