Standardized flow cytometry gating in veterinary medicine.
Gating in flow cytometry is used to select subpopulations of cells for analysis. The technique is critical for subsequent analysis in order to select the population, free of debris and unrelated cells. Accurately quantifying subpopulations in clinical cases is necessary for correct diagnosis. Human lymphocytes are selected by backgating on populations of CD45+high CD14- cells. These reagents are not available widely across species. In veterinary medicine, markers to identify lymphocytes are usually limited to T-lymphocyte, CD4, CD8, and B-lymphocyte surface antigens. A standardized gating technique using a T-lymphocyte antibody is described and is applicable across species where limited phenotype markers are available.
Byrne
K M
KM
Department of Animal Sciences, Washington State University, P.O. Box 646351, Pullman, WA 99164-6351, USA. kbyrne@wsu.edu
Reinhart
G A
GA
Hayek
M G
MG
D000818:Animals; D000961:Antilymphocyte Serum / Q000032:analysis*; D002415:Cats; D004285:Dogs; D005434:Flow Cytometry / Q000379:methods* / Q000662:veterinary; D016131:Lymphocyte Subsets / Q000145:classification / Q000166:cytology*; D012015:Reference Standards; D013601:T-Lymphocytes / Q000166:cytology*; D014730:Veterinary Medicine
D000961:Antilymphocyte Serum