Construction of promoter-probe shuttle vectors for Escherichia coli and corynebacteria on the basis of promoterless alpha-amylase gene.
We constructed new promoter-probe vectors for E. coli and corynebacteria based on the promoterless alpha-amylase gene originating from Bacillus subtilis. Vectors pJUPAE1 and pJUPAE2 are suitable for isolation of transcriptionally active fragments from plasmids, phages or genomic DNA. alpha-Amylase activity can be easily visually detected on agar plates containing a chromogenic substrate, or by direct measurement of alpha-amylase activity.
Institute of Molecular Biology, Slovak Academy of Sciences, 842 51 Bratislava, Slovakia.
J
J
Ugorcáková
G
G
Bukovská
J
J
Timko
D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics; D003352:Corynebacterium / Q000235:genetics*; D015342:DNA Probes; D004926:Escherichia coli / Q000235:genetics*; D005822:Genetic Vectors; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D014169:Transformation, Bacterial; D000516:alpha-Amylases / Q000235:genetics* / Q000378:metabolism
D015342:DNA Probes; D000516:alpha-Amylases