In vivo microdialysis in the visual cortex of awake cat. I: surgery, animal training and sampling.
Sampling and monitoring release of excitatory and inhibitory amino acids in the striate cortex of mammals will provide important information for visual system research. A method allowing repeated microdialysis in the cortical layers of area 17 of the awake cat is described. Under visual control through a surgical microscope and using a stereotactic instrument, four probe guides are permanently implanted in area 17 of one hemisphere of the anesthetized animal and two fixation bars are mounted on the skull to allow fixation of the cat in a stereotactic frame. The implantation of four probe guides in the same hemisphere allows simultaneous sampling from different cortical regions subserving different parts of the visual field. A removable transparent cover protects the probe guides. After recovery from surgery the awake cats are trained to adapt to a fixation in a stereotaxic apparatus. Once adapted to that situation, the cats are used for 5 h in vivo microdialysis experiments without anesthesia.
Laboratory of Neuroendocrinology and Immunological Biotechnology, Zoological Institute, Katholieke Universiteit Leuven, B-3000, Leuven, Belgium. quying@mail.nih.gov
Y
Y
Qu
L
L
Arckens
F
F
Vandesande
E
E
Vandenbussche
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002415:Cats; D005110:Extracellular Space / Q000378:metabolism; D005260:Female; D008297:Male; D017551:Microdialysis / Q000379:methods*; D012016:Reference Values; D012683:Sensory Deprivation / Q000502:physiology; D013238:Stereotaxic Techniques; D015349:Vision, Monocular / Q000502:physiology; D014793:Visual Cortex / Q000378:metabolism*
D000596:Amino Acids