Increased ribonucleotide reductase activity in hydroxyurea-resistant mosquito cells.
Hydroxyurea-resistant Aedes albopictus mosquito cells were selected by incremental exposure of unmutagenized cells to hydroxyurea concentrations ranging from 0.1 to 8 mM. Clonal populations that had become 40-fold more resistant to hydroxyurea than wild-type cells varied in morphology, and their growth rate decreased to a;45 h doubling time, relative to an 18 h doubling time in unselected cells. At this level of resistance, the cells remained diploid, with a modal chromosome number of 6. When labelled with (35)S[methionine/cysteine], clone HU1062, which grew in the presence of 8 mM hydroxyurea, overproduced a labeled protein with the approximate size of the 45,000 dalton M2 subunit of ribonucleotide reductase. Consistent with this observation, ribonucleotide reductase activity in HU-1062 cells was approximately 10-fold higher than in wild-type control cells. This is the first example of an hydroxyurea-resistant insect cell line. [Originally published in Volume 34, Archives of Insect Biochemistry and Physiology, 34:31-41 (1997).]
Department of Entomology, University of Minnesota, St. Paul, MN 55108, USA.
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D000330:Aedes / Q000201:enzymology* / Q000502:physiology; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D004351:Drug Resistance / Q000235:genetics; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006918:Hydroxyurea / Q000494:pharmacology*; D007621:Karyotyping; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis; D012264:Ribonucleotide Reductases / Q000378:metabolism*; D012641:Selection, Genetic
D004791:Enzyme Inhibitors; D011506:Proteins; D012264:Ribonucleotide Reductases; D006918:Hydroxyurea