Environmental and nutritional factors affecting geosmin synthesis by Anabaena sp.
A cyanobacterium isolated from a source-water reservoir during a spring odor and taste episode and identified as Anabaena sp. consistently produced geosmin during laboratory culture on modified BG-11 liquid medium. Maximal geosmin/biomass occurred at 20 degrees C and a light intensity of 17 microE/m2/s; geosmin/chla values directly correlated with increasing light intensity (r2 = 0.95, P < 0.01). It was concluded that at 20 degrees C, increasing light intensity favors less chla synthesis and higher geosmin synthesis; at 17 microE/m2/s, increasing temperature stimulates chla production (to 25 degrees C) while repressing geosmin synthesis (above 20 degrees C). Nutritional factors promoting biomass, chla, and geosmin synthesis by Anabaena sp. were also investigated. For cultures grown at 17 microE/m2/s and 20 degrees C for 20 days, both ammonium-N and nitrate-N generally enhanced the growth of Anabaena sp. Nitrate-N promoted more chla production (r2 = 0.99) than ammonium-N. Geosmin synthesis was directly correlated with ammonium-N concentrations (r2 = 0.89), with low nitrate-N (123.5 micrograms/l) favoring maximal geosmin production (2.8 micrograms/l). Increasing nitrate-N concentrations promoted a three-fold increase in chla content with geosmin synthesis decreased by two-fold. Geosmin/mg biomass was directly related to ammonium-N concentration; high nitrate-N levels suppressed geosmin production. No geosmin was detected at or below 118 micrograms phosphate-phosphorus/l. Geosmin, dry weight biomass, and chla production were correlated with increasing phosphorus (P) concentration (r2 = 0.76, 0.96 and 0.98, respectively). No geosmin was detected when copper was present in growth media at or above 6.92 micrograms Cu2+/l (CuSO4.5H2O). Dry weight biomass and chla production were negatively correlated with Cu2+ ion concentrations.
Department of Biological Sciences, Jordan University of Science and Technology, P.O. Box 3030, Irbid-22110, Jordan. issadoun@just.edu.jo
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D017033:Anabaena / Q000254:growth & development / Q000378:metabolism*; D001673:Biodegradation, Environmental; D018533:Biomass; D002734:Chlorophyll / Q000378:metabolism; D000077194:Chlorophyll A; D003300:Copper / Q000378:metabolism; D007700:Kinetics; D008027:Light; D009284:Naphthols / Q000378:metabolism*; D009566:Nitrates / Q000378:metabolism; D009812:Odorants; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D012621:Seasons; D013649:Taste; D014871:Water Microbiology; D014881:Water Supply / Q000592:standards*
D009284:Naphthols; D009566:Nitrates; D000644:Quaternary Ammonium Compounds; D002734:Chlorophyll; D003300:Copper; C001278:geosmin; D000077194:Chlorophyll A