Effect of cold aerobic perfusion on nonparenchymal cell viability of rat livers.
We investigated the effect of oxygen supply on hepatic cellular viability during cold perfusion storage of rat livers. A perfluoro-N-methyldecahydroisoquinoline (FMIQ) emulsion is used as an oxygen carrier. The composition of the perfusate containing 20 w/v% FMIQ is essentially the same as the University of Wisconsin (UW) solution except for the exclusion of hydroxyethyl starch. Rat livers were perfused at 4 degrees C for up to 24 h with either UW solution (group I, oxygenated; group II, unoxygenated) or FMIQ solution (group III, oxygenated; group IV, unoxygenated). After perfusion storage, the livers were reperfused with warm (37 degrees C) oxygenated or cold (4 degrees C) unoxygenated Krebs-Henseleit bicarbonate buffer, and nuclear trypan blue uptake was measured as the index of cell death. With warm oxygenated reperfusion, there remained less than 2% noviable parenchymal cells up to 24 h, regardless of perfusate or oxygenation. In UW-perfused livers, the proportion of nonviable nonprenchymal cells (NPC) increased progressively regardless of oxygenation, the values in groups I and II in the periportal field at 24 h being 39.9 +/- 4.7% (mean +/- SD) and 36.5 +/- 4.2%, respectively. By contrast, in FMIQ-perfused livers, dye uptake by NPC was significantly reduced with oxygenation (16.9 +/- 5.7% and 39.4% +/- 9.1% at 24 h in groups III and IV; P < 0.001). With cold unoxygenated reperfusion, livers in groups I, II, and IV showed a significant decrease of nonviable NPC, while those in group III showed no significant changes. These data indicate that oxygen supply during perfusion storage of the liver may ameliorate lethal injury to NPC precipitated during reperfusion.
Ku
Y
Y
First Department of Surgery, Kobe University School of Medicine, Japan.
Fukumoto
T
T
Samizo
M
M
Maekawa
Y
Y
Nishida
T
T
Shiki
H
H
Tominaga
M
M
Kuroda
Y
Y
Saitoh
Y
Y
D000241:Adenosine / Q000494:pharmacology; D000332:Aerobiosis; D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D001802:Blood Substitutes / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D005466:Fluorocarbons / Q000494:pharmacology*; D005978:Glutathione / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D007546:Isoquinolines / Q000494:pharmacology*; D008099:Liver / Q000166:cytology* / Q000187:drug effects; D008297:Male; D019188:Organ Preservation Solutions / Q000494:pharmacology; D010477:Perfusion / Q000379:methods; D011887:Raffinose / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015424:Reperfusion; D014343:Trypan Blue / Q000493:pharmacokinetics
D001802:Blood Substitutes; D005466:Fluorocarbons; D007328:Insulin; D007546:Isoquinolines; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; C065081:perfluoro-N-methyldecahydroisoquinoline; D005978:Glutathione; D014343:Trypan Blue; D000241:Adenosine; D011887:Raffinose