Postembryonic proliferation in the spiny lobster antennular epithelium: rate of genesis of olfactory receptor neurons is dependent on molt stage.
Olfactory systems undergo continuous growth and turnover in many animals. Many decapod crustaceans, such as lobsters and crayfish, have indeterminate growth, and in these animals, turnover of both peripheral and central components of the olfactory system occurs continuously throughout life. In this study, we examine the dynamics of olfactory receptor neuron (ORN) proliferation in the antennule of the Caribbean spiny lobster, Panulirus argus, using in vivo incorporation of the cell proliferation marker BrdU. We show that addition of ORNs occurs in a "proximal proliferation zone" (PPZ), which exists on the proximo-lateral margin of the existing ORN population. The PPZ is spatially and temporally dynamic in that it travels as a wave in the proximal and lateral directions in the antennule. This wave results in continuous addition of ORNs throughout the molt cycle. The rate of proliferation, as measured by the size and shape of the PPZ, changes depending on the animal's molt stage. The rate is highest during premolt and lowest during intermolt. ORNs are the most prominent cell-type produced in the PPZ, but other cell types, including glia, are also produced. Patches of proliferating epithelial cells occur immediately proximal to the PPZ, suggesting that neuronal and glial precursors reside in this region. Possible mechanisms for peripheral and central modulation of ORN development are discussed.
Harrison
P J
PJ
Department of Biology, Georgia State University, P.O. Box 4010, Atlanta, GA 30302-4010, USA. pjh@gsu.edu
Cate
H S
HS
Swanson
E S
ES
Derby
C D
CD
D000818:Animals; D001973:Bromodeoxyuridine; D002455:Cell Division / Q000502:physiology; D005121:Extremities / Q000033:anatomy & histology / Q000294:innervation; D018978:Molting / Q000502:physiology; D008121:Nephropidae / Q000033:anatomy & histology / Q000254:growth & development / Q000502:physiology*; D009457:Neuroglia / Q000166:cytology; D009831:Olfactory Mucosa / Q000166:cytology / Q000294:innervation*; D018034:Olfactory Receptor Neurons / Q000166:cytology*
D001973:Bromodeoxyuridine