Induction of a virus-specific antibody response to foot and mouth disease virus using the structural protein VP1 expressed in transgenic potato plants.
We have recently communicated the oral and parental immunogenicity of the structural protein VP1 of foot and mouth disease virus (FMDV) expressed in different transgenic plants. Those results clearly indicated the necessity of increasing the expression of the foreign genes in the transgenic plant to avoid additional steps toward the purification and/or concentration of the antigen of interest. Here, we report the production of transgenic potatoes plants containing the VP1 gene cloned under the regulatory activity of either a single (pRok2) or a double (pRok3) copy of the S35 cauliflower mosaic virus (CaMV 35S) promoter, as a strategy for increasing the level of VP1 gene expression. The presence of the VP1 gene in the plants was confirmed by polymerase chain reaction (PCR) and its specific transcription activity was demonstrated by reverse transcriptase-polymerase chain reaction (RT-PCR). The results showed that, although the immunized animals presented a FMDV VP1 specific antibody response and protection against the experimental challenge, no significant differences were demonstrated in the immunizing activity of plant extracts obtained from the pRok2 or pRok3 transformed plants. These results confirm those previously obtained using other plant species allowing the possibility of using plants as antigen expression vectors, and demonstrated that at least in the potato system, the use of double CaMV 35S promoter does not cause a significant increase in the level of the VP1 expressed.
Carrillo
C
C
Instituto de Virología, CICV, INTA-Castelar, Buenos Aires, Argentina.
Wigdorovitz
A
A
Trono
K
K
Dus Santos
M J
MJ
Castañón
S
S
Sadir
A M
AM
Ordas
R
R
Escribano
J M
JM
Borca
M V
MV
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D005537:Aphthovirus / Q000276:immunology*; D002213:Capsid / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D036022:Capsid Proteins; D005536:Foot-and-Mouth Disease / Q000276:immunology* / Q000517:prevention & control; D005814:Genes, Viral; D007114:Immunization; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D030821:Plants, Genetically Modified / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D011994:Recombinant Proteins / Q000276:immunology; D011198:Solanum tuberosum / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D014170:Transformation, Genetic; D014765:Viral Vaccines
D000914:Antibodies, Viral; D036022:Capsid Proteins; D011994:Recombinant Proteins; C442501:VP1 protein, Foot-and-mouth disease virus; D014765:Viral Vaccines