RcoA has pleiotropic effects on Aspergillus nidulans cellular development.
Aspergillus nidulans rcoA encodes a member of the WD repeat family of proteins. The RcoA protein shares sequence similarity with other members of this protein family, including the Saccharomyces cerevisiae Tup1p and Neurospora crassa RCO1. Tup1p is involved in negative regulation of an array of functions including carbon catabolite repression. RCO1 functions in regulating pleiotropic developmental processes, but not carbon catabolite repression. In A. nidulans, deletion of rcoA (DeltarcoA), a recessive mutation, resulted in gross defects in vegetative growth, asexual spore production and sterigmatocystin (ST) biosynthesis. Expression of the asexual and ST pathway-specific regulatory genes, brlA and aflR, respectively, but not the signal transduction genes (i.e. flbA, fluG or fadA) regulating brlA and aflR expression was delayed (brlA) or eliminated (aflR) in a DeltarcoA strain. Overexpression of aflR in a DeltarcoA strain could not rescue normal expression of downstream targets of AflR. CreA-dependent carbon catabolite repression of starch and ethanol utilization was only weakly affected in a DeltarcoA strain. The strong role of RcoA in development, vegetative growth and ST production, compared with a relatively weak role in carbon catabolite repression, is similar to the role of RCO1 in N. crassa.
Department of Plant Pathology and Microbiology, TAMUS 2132, Texas A&M University, College Station, TX 77843-2132, USA.
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J
Hicks
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Lockington
J
J
Strauss
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Dieringer
C P
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Kubicek
J
J
Kelly
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Keller
D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D019204:GTP-Binding Proteins; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D008969:Molecular Sequence Data; D020449:Repetitive Sequences, Amino Acid; D012097:Repressor Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D013241:Sterigmatocystin / Q000096:biosynthesis; D014157:Transcription Factors
C113837:AFLR protein, Aspergillus; C063497:BRLA protein, Emericella nidulans; D004268:DNA-Binding Proteins; C102142:FADA protein, Emericella nidulans; C089966:FLUG protein, Emericella nidulans; D005656:Fungal Proteins; C430110:RcoA protein, Aspergillus nidulans; D012097:Repressor Proteins; D014157:Transcription Factors; C091553:flbA protein, Aspergillus nidulans; D013241:Sterigmatocystin; C071237:CreA protein, Aspergillus nidulans; D002244:Carbon; D019204:GTP-Binding Proteins