Amine oxidase-like activity of polyphenols. Mechanism and properties.
Polyphenols in several oxidation systems gained amine oxidase-like activity, probably due to the formation of the corresponding quinones. In the presence of Cu(II), o- and p-phenolic compounds exhibited amine oxidase-like activity, whereas only the o-phenolic compounds showed the activity in the presence of 1,1-diphenyl-2-picrylhydrazyl radical. The activity was determined by measuring the conversion of benzylamine to benzaldehyde by HPLC. Moreover, gallic acid, chlorogenic acid, and caffeic acid, which are plant polyphenols, converted the lysine residue of bovine serum albumin to alpha-amino-adipic semialdehyde residue, indicating lysyl oxidase-like activity. We also characterized the activity of pyrocatechol, hydroquinone, and pyrogallol in the presence of Cu(II). The oxidative deamination was accelerated at a higher pH, and required O2 and transition metal ions. Furthermore, EDTA markedly inhibited the reaction but not beta-aminopropionitrile, which is a specific inhibitor of lysyl oxidase. Catalase significantly inhibited the oxidation, implying the participation of hydroxyl radical in the reaction, but superoxide dismutase stimulated the oxidation, probably due to its radical formation activity. We discussed the mechanism of the oxidative deamination by polyphenols and the possible significance of the activity for biological systems.
Department of Applied Bioorganic Chemistry, Division of Life Science, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
M
M
Akagawa
K
K
Suyama
D006631:Amine Oxidase (Copper-Containing) / Q000737:chemistry / Q000378:metabolism*; D000629:Aminopropionitrile / Q000494:pharmacology; D001596:Benzylamines / Q000378:metabolism; D002109:Caffeic Acids / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002396:Catechols / Q000378:metabolism; D002614:Chelating Agents / Q000494:pharmacology; D002726:Chlorogenic Acid / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003300:Copper / Q000378:metabolism; D004492:Edetic Acid / Q000494:pharmacology; D005419:Flavonoids; D005609:Free Radicals; D005707:Gallic Acid / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006873:Hydroquinones / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D008956:Models, Chemical; D010636:Phenols / Q000737:chemistry / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry / Q000378:metabolism*; D008249:Protein-Lysine 6-Oxidase / Q000378:metabolism; D011748:Pyrogallol / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013329:Structure-Activity Relationship; D013482:Superoxide Dismutase / Q000378:metabolism
D001596:Benzylamines; D002109:Caffeic Acids; D002396:Catechols; D002614:Chelating Agents; D005419:Flavonoids; D005609:Free Radicals; D006873:Hydroquinones; D010636:Phenols; D011108:Polymers; D011748:Pyrogallol; D000629:Aminopropionitrile; D012710:Serum Albumin, Bovine; D002726:Chlorogenic Acid; D005707:Gallic Acid; D003300:Copper; D004492:Edetic Acid; D002374:Catalase; D013482:Superoxide Dismutase; D008249:Protein-Lysine 6-Oxidase; D006631:Amine Oxidase (Copper-Containing); D008239:Lysine; C034221:catechol; C040048:caffeic acid; C031927:hydroquinone