Enterocin P selectively dissipates the membrane potential of Enterococcus faecium T136.
Enterocin P is a pediocin-like, broad-spectrum bacteriocin which displays a strong inhibitory activity against Listeria monocytogenes. The bacteriocin was purified from the culture supernatant of Enterococcus faecium P13, and its molecular mechanism of action against the sensitive strain E. faecium T136 was evaluated. Although enterocin P caused significant reduction of the membrane potential (DeltaPsi) and the intracellular ATP pool of the indicator organism, the pH gradient (DeltapH) component of the proton motive force (Deltap) was not dissipated. By contrast, enterocin P caused carboxyfluorescein efflux from E. faecium T136-derived liposomes.
Departmento de Nutrición y Bromatología III, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.
C
C
Herranz
Y
Y
Chen
H J
HJ
Chung
L M
LM
Cintas
P E
PE
Hernández
T J
TJ
Montville
M L
ML
Chikindas
D000255:Adenosine Triphosphate / Q000378:metabolism; D001430:Bacteriocins / Q000378:metabolism / Q000494:pharmacology*; D016984:Enterococcus faecium / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology*; D005452:Fluoresceins / Q000378:metabolism; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D008081:Liposomes / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects
D001430:Bacteriocins; D005452:Fluoresceins; D008081:Liposomes; C417717:enterocin P; C024098:6-carboxyfluorescein; D000255:Adenosine Triphosphate