Schizosaccharomyces pombe taf1+ is required for nitrogen starvation-induced sexual development and for entering the dormant GO state.
Environmental change, such as nutritional starvation, induces physiological and morphological alterations that enable fission yeast cells to survive. We isolated a novel gene, taf1+, required for the response to nitrogen starvation in the fission yeast Schizosaccharomyces pombe. taf1 disruptants could not mate upon nitrogen starvation, but could upon carbon starvation. taf1 disruptants had a defect in inducing stell+ expression under nitrogen starvation conditions. Furthermore, they lost viability quickly in nitrogen-depleted medium. Unlike wild-type cells, starved taf1-cells had nuclear chromatin that were flat and adhered to the cell periphery. These results indicate that tqf1+ is required for nitrogen starvation-induced sexual development and entering the dormant G0 state.
Ueno
M
M
Department of Chemistry, Faculty of Sciences, Shizuoka University, Japan. scmueno@ipc.shizuoka.ac.jp
Kurokawa
R
R
Renauld
H
H
Watanabe
K
K
Ushimaru
T
T
Uritani
M
M
Yoshinaga
K
K
Hiraoka
Y
Y
D000595:Amino Acid Sequence; D000071183:Autophagy-Related Proteins; D002868:Chromosomal Proteins, Non-Histone; D005656:Fungal Proteins / Q000235:genetics; D017353:Gene Deletion; D015870:Gene Expression; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism*; D009586:Nitrogen Fixation / Q000235:genetics / Q000502:physiology*; D009687:Nuclear Proteins / Q000235:genetics*; D016192:Resting Phase, Cell Cycle; D012568:Schizosaccharomyces / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors / Q000235:genetics
D000071183:Autophagy-Related Proteins; D002868:Chromosomal Proteins, Non-Histone; D005656:Fungal Proteins; D009687:Nuclear Proteins; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors; C513475:atg11 protein, S pombe; C071596:ste11 protein, S pombe; D009584:Nitrogen