In vitro differentiation of human marrow stromal cells into early progenitors of neural cells by conditions that increase intracellular cyclic AMP.
Human marrow stromal cells (hMSCs) are multipotential stem cells that can be differentiated into bone, cartilage, fat, and muscle. In the experiments here, we found that undifferentiated cultures of hMSCs express some markers characteristic of neural cells such as microtubule-associated protein 1B (MAP1B), neuron-specific tubulin (TuJ-1), neuron-specific enolase (NSE), and vimentin. By treating hMSCs with 0.5 mM isobutylmethylxanthine (IBMX)/1 mM dibutyryl cyclic AMP (dbcAMP) for 6 days, about 25% of the hMSCs differentiated into cells with a typical neural cell morphology and with increased levels of both NSE and vimentin. The data suggested that the hMSCs may have been differentiated into early progenitors of neural cells in vitro under conditions that increase the intracellular level of cAMP.
Center for Gene Therapy, Tulane University Health Sciences Center, SL-99, 1430 Tulane Avenue, New Orleans, Louisiana, 70112, USA.
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W
Deng
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Obrocka
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I
Fischer
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DJ
Prockop
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000293:Adolescent; D000328:Adult; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D003994:Bucladesine / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000096:biosynthesis / Q000378:metabolism*; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D008875:Middle Aged; D009420:Nervous System / Q000166:cytology* / Q000378:metabolism; D010641:Phenotype; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D017154:Stromal Cells / Q000166:cytology* / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism
D008869:Microtubule-Associated Proteins; D014404:Tubulin; D014746:Vimentin; C085284:microtubule-associated protein 1B; D003994:Bucladesine; D000242:Cyclic AMP; D010751:Phosphopyruvate Hydratase; D015056:1-Methyl-3-isobutylxanthine