Bilobalide promotes expression of glial cell line-derived neurotrophic factor and vascular endothelial growth factor in rat astrocytes.
OBJECTIVE
To study the effects of bilobalide on the expression of glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF) in rat astrocytes in vitro.


METHODS
Semiquantification polymerase chain reaction (SQ-PCR) was used to investigate GDNF and VEGF mRNA expression in the astrocytes after bilobalide (5, 15, 50, 100 mumol.L-1) treatment. Immunohistochemistry method was used to detect GDNF and VEGF protein expression in cells treated with bilobalide 50 mumol.L-1 for 24 h.


RESULTS
GDNF and VEGF mRNA increased markedly after astrocytes were treated with bilobalide 50 mumol.L-1 for 12 h. GDNF and VEGF protein were detected in the cytoplasm of astrocytes after the cells were treated with bilobalide 50 mumol.L-1 for 24 h.


CONCLUSIONS
Bilobalide induced GDNF and VEGF expression in the cultured astrocytes.
Zheng
S X
SX
Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 200031, China.
Zhou
L J
LJ
Chen
Z L
ZL
Yin
M L
ML
Zhu
X Z
XZ
D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D002460:Cell Line; D003517:Cyclopentanes / Q000302:isolation & purification / Q000494:pharmacology*; D004224:Diterpenes; D016228:Endothelial Growth Factors / Q000096:biosynthesis* / Q000235:genetics; D005663:Furans / Q000302:isolation & purification / Q000494:pharmacology*; D020441:Ginkgo biloba / Q000737:chemistry; D046934:Ginkgolides; D008222:Lymphokines / Q000096:biosynthesis* / Q000235:genetics; D020890:Neuregulin-1 / Q000096:biosynthesis* / Q000235:genetics; D010946:Plants, Medicinal; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D003517:Cyclopentanes; D004224:Diterpenes; D016228:Endothelial Growth Factors; D005663:Furans; D046934:Ginkgolides; D008222:Lymphokines; D020890:Neuregulin-1; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; C073710:bilobalide