In vivo near-infrared monitoring of nitric oxide production and tissue oxygen sufficiency in rat liver allografts during the acute rejection reaction.
We established a new technique of in vivo near-infrared (NIR) spectroscopy that can estimate both nitric oxide (NO) production and tissue oxygen sufficiency in living organs during the alloimmune response. The present study was aimed at evaluating the potential of this technique for monitoring the rejection response utilizing the rat model of orthotopic liver transplantation without arterialization. The relative changes of nitrosyl-hemoglobin, oxyhemoglobin and oxidized-cytochrome oxidase in the graft livers were quantified by use of this method. Nitrosyl-hemoglobin in the allogenic grafts was elevated at the onset of the rejection response and was suppressed when the rejection reaction was treated by the administration of 15-deoxyspergualin. Oxy-hemoglobin and oxidized-cytochrome oxidase were decreased in accordance with parenchymal disorder determined histologically. These results demonstrated that the new technique of in vivo NIR spectroscopy can assess simultaneously both the immune response and graft function after liver transplantation.
Ohdan
H
H
Second Department of Surgery, Hiroshima University, School of Medicine, Japan.
Suzuki
S
S
Kanashiro
M
M
Amemiya
H
H
Fukuda
Y
Y
Dohi
K
K
D000818:Animals; D003576:Electron Transport Complex IV / Q000032:analysis; D006084:Graft Rejection / Q000175:diagnosis* / Q000473:pathology; D006146:Guanidines / Q000627:therapeutic use; D006454:Hemoglobins / Q000032:analysis*; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D016031:Liver Transplantation / Q000473:pathology / Q000502:physiology*; D008297:Male; D008991:Monitoring, Physiologic / Q000379:methods; D009566:Nitrates / Q000097:blood*; D009569:Nitric Oxide / Q000378:metabolism*; D010108:Oxyhemoglobins / Q000032:analysis; D051381:Rats; D011912:Rats, Inbred ACI; D011917:Rats, Inbred Lew; D013055:Spectrophotometry, Infrared / Q000295:instrumentation / Q000379:methods; D014184:Transplantation, Homologous; D014185:Transplantation, Isogeneic
D006146:Guanidines; D006454:Hemoglobins; D007166:Immunosuppressive Agents; D009566:Nitrates; D010108:Oxyhemoglobins; C008657:nitrosyl hemoglobin; D009569:Nitric Oxide; D003576:Electron Transport Complex IV; C037258:gusperimus