Expression of nerve growth factor and its receptors in the somatosensory-motor cortex of Macaca nemestrina.
The present study was designed to examine the nerve growth factor (NGF) system (ligand and receptor-expressing neurons) in the somatosensory (areas 1, 3a, and 3b) and motor (area 4) cortices of the mature macaque. Light and electron microscope immunohistochemistry was used to assess the distribution and identity of NGF-, p75-, and trk-expressing elements. In each cortical area examined, NGF-positive neuronal somata were distributed through all laminae; most immunolabeled neurons were in layers II, III, and V. Based upon light microscope criteria (e.g., the morphology of proximal dendrites), both pyramidal and stellate neurons expressed NGF. Of the identifiable NGF- immunoreactive cells, 92% were pyramidal neurons and the remainder was stellate neurons. The electron microscope study showed that most (88%) NGF-positive somata formed symmetric synapses, whereas the others formed both symmetric and asymmetric synapses. As the somata of pyramidal neurons form only symmetric synapses and those of inhibitory stellate neurons form both symmetric and asymmetric somatic synapses, the ultrastructural data support the light microscopic analyses. In contrast, neurotrophin receptors, p75 and trk, were expressed chiefly by the cell bodies of layer V pyramidal neurons and the supragranular neuropil. At the ultrastructural level, receptor-positive profiles were post-synaptic elements (e.g., dendritic shafts and spines) and the concentration of immunoreactivity was greatest in the vicinity of post-synaptic densities. Thus, NGF regulatory systems parallel excitatory and inhibitory neurotransmitter systems. Cortex contains the morphological framework by which pyramidal and/or inhibitory stellate neurons can affect the activity of post-synaptic pyramidal neurons via anterograde and autocrine/paracrine NGF systems.
Research Service, Veterans Affairs Medical Center, Iowa City IA 52246-2208, USA.
M W
MW
Miller
D000544:Alzheimer Disease; D000818:Animals; D019898:Autocrine Communication; D001370:Axonal Transport; D007150:Immunohistochemistry; D008254:Macaca nemestrina / Q000378:metabolism*; D008854:Microscopy, Electron; D009044:Motor Cortex / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D020932:Nerve Growth Factor / Q000276:immunology / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism / Q000648:ultrastructure; D019899:Paracrine Communication; D020794:Receptor Protein-Tyrosine Kinases / Q000276:immunology / Q000378:metabolism; D017475:Receptors, Nerve Growth Factor / Q000276:immunology / Q000378:metabolism*; D013003:Somatosensory Cortex / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure
D017475:Receptors, Nerve Growth Factor; D020932:Nerve Growth Factor; D020794:Receptor Protein-Tyrosine Kinases