[Rh system genotyping in amniotic fluid].
The aim of this work was to determine the presence of the RHD gene in fetal cells obtained from amniotic fluid (AF). We studied 65 samples of AF, 11 from RhD- mothers sensitized with anti-D. The fetal origin of the DNA was confirmed with the analysis of 1 VNTR locus and 3 STR loci in DNA samples from AF and maternal blood. The RHD genotyping was performed in non contaminated samples (n = 62) using a multiplex PCR strategy that yields 3 amplification products from RhD+ phenotypes and 1 DNA fragment from RhD- phenotypes. We genotyped 54 RhD+ fetuses (8 from RhD- sensitized mothers) and 8 RhD- fetuses (3 from RhD- sensitized mothers). Fetal DNA genotyping allows the diagnosis, from a single amniocentesis, of fetuses at real risk of hemolytic disease of the newborn. When the fetus is determined to be RhD- all invasive procedures can be avoided.
Area Inmunología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, 2000 Rosario, Argentina. ccottorru@fbioyf.unr.edu.ar
C
C
Cotorruelo
C
C
Biondi
S
S
García Borrás
R
R
Di Mónaco
W
W
Martino
A
A
Racca
D000653:Amniotic Fluid / Q000276:immunology*; D004247:DNA / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D004899:Erythroblastosis, Fetal / Q000175:diagnosis* / Q000235:genetics; D005260:Female; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012204:Rh-Hr Blood-Group System / Q000235:genetics*
D005819:Genetic Markers; D012204:Rh-Hr Blood-Group System; D004247:DNA