Prediction of affinity and kinetics in biomolecular interactions by affinity chromatography.
Computer simulation of affinity chromatography is a valuable tool for accurate prediction of column performance. In our study affinity pairs based on lectin and antibody interactions with carbohydrates have been used as model systems. In this well-characterized system we have demonstrated the usefulness of the simulation approach for determination of affinity and kinetics. These properties are typically difficult to obtain for many weakly interacting molecular species (i.e., when dissociation constants (K(D)) are greater than 10(-5) M). The influence of affinity and kinetics on peak broadening in affinity chromatography has also been investigated.
Department of Chemistry and Biomedical Sciences, University of Kalmar, Kalmar, SE-391 82, Sweden. Lisa.Leickt@hik.se
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Leickt
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Månsson
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Ohlson
D000117:Acetylglucosamine / Q000032:analysis* / Q000737:chemistry; D000911:Antibodies, Monoclonal / Q000737:chemistry; D001665:Binding Sites; D002846:Chromatography, Affinity / Q000379:methods*; D003198:Computer Simulation; D007700:Kinetics; D008320:Maltose / Q000032:analysis* / Q000737:chemistry; D014909:Wheat Germ Agglutinins / Q000737:chemistry
D000911:Antibodies, Monoclonal; D014909:Wheat Germ Agglutinins; D008320:Maltose; D000117:Acetylglucosamine