Sequence and peptide-binding motif for a variant of HLA-A*0214 (A*02142) in an HIV-1-resistant individual from the Nairobi Sex Worker cohort.
As part of the ongoing study of natural HIV-1 resistance in the women of the Nairobi Sex Workers' study, we have examined a resistance-associated HLA class I allele at the molecular level. Typing by polymerase chain reaction using sequence-specific primers determined that this molecule is closely related to HLA-A*0214, one of a family of HLA-A2 supertype alleles which correlate with HIV-1 resistance in this population. Direct nucleotide sequencing shows that this molecule differs from A*0214, having a silent nucleotide substitution. We therefore propose to designate it HLA-A*02142. We have determined the peptide-binding motif of HLA-A*0214/02142 by peptide elution and bulk Edman degradative sequencing. The resulting motif, X-[Q,V]-X-X-X-K-X-X-[V,L], includes lysine as an anchor at position 6. The data complement available information on the peptide-binding characteristics of this molecule, and will be of use in identifying antigenic peptides from HIV-1 and other pathogens.
Luscher
M A
MA
Department of Medicine, University of Toronto, Canada.
MacDonald
K S
KS
Bwayo
J J
JJ
Plummer
F A
FA
Barber
B H
BH
D000595:Amino Acid Sequence; D001665:Binding Sites / Q000235:genetics; D015331:Cohort Studies; D005260:Female; D015658:HIV Infections / Q000276:immunology*; D018023:HIV Seronegativity / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D015789:HLA-A2 Antigen / Q000235:genetics*; D006684:HLA-DR Antigens; D006801:Humans; D007630:Kenya; D008969:Molecular Sequence Data; D009154:Mutation; D011477:Sex Work
C423265:HLA-A*02:14 antigen; D015789:HLA-A2 Antigen; D006684:HLA-DR Antigens