High molecular weight dextran sulphate enhances the activity of NF-kappaB-regulated promoter in monocytic cell line.
It has been known that lipopolysaccharides (LPS) as well as superantigens increase activity of transcription factor NF-kappaB in monocyte-derived macrophages and monocytic cell lines. To examine the mitogenic effect of high molecular weight dextran sulphate (HMDS), the monocytic cell line THP-1 was transfected with a plasmid vector containing chloramphenicol acetyl transferase (CAT) reporter gene linked to the HIV-1 long terminal repeat (LTR) promoter, that is regulated by the transcription factor NF-kappaB. We have demonstrated that HMDS, similar to bacterial LPS, increases the expression of CAT reporter gene, indicating increased activity of NF-kappaB.
Department of Biochemistry and Molecular Biology, University of Medical Sciences, Poznań, Poland. pjagodzi@mail.usoms.poznan.pl
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Trzeciak
D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D016264:Dextran Sulfate / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D017930:Genes, Reporter; D015497:HIV-1 / Q000235:genetics; D006801:Humans; D009000:Monocytes / Q000166:cytology / Q000502:physiology* / Q000821:virology; D016328:NF-kappa B / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000187:drug effects*; D014162:Transfection
D000998:Antiviral Agents; D016328:NF-kappa B; D016264:Dextran Sulfate; D015500:Chloramphenicol O-Acetyltransferase