Structural analysis reflects the evolutionary relationship between the human desmocollin gene family members.
Desmocollins, members of the desmosomal cadherin family, are known to play an important role in desmosomal intercellular adhesion. The human desmosomal cadherin cluster is located on chromosome 18q12, and consists of three desmoglein and three desmocollin genes. The cDNAs of all six of these genes have been cloned and sequenced, however, the exon-intron organization was reported for only one human desmocollin gene, DSC2. We elucidated the exon-intron structures of the DSC1 and DSC3 genes using PCR amplification of genomic DNA and direct sequencing of BAC clones. The results suggest a strong evolutionary conservation between the genomic organization of the desmocollin genes.
Department of Dermatology, Columbia University, 630 W. 168th Street VC-1526, New York, NY 10032, USA.
P B
PB
Cserhalmi-Friedman
J A
JA
Frank
W
W
Ahmad
A A
AA
Panteleyev
V M
VM
Aita
A M
AM
Christiano
D000328:Adult; D000818:Animals; D002448:Cell Adhesion; D002887:Chromosomes, Human, Pair 18 / Q000235:genetics*; D003598:Cytoskeletal Proteins / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D051187:Desmocollins; D051182:Desmogleins; D051180:Desmoplakins; D003896:Desmosomes / Q000737:chemistry; D019143:Evolution, Molecular; D005091:Exons / Q000235:genetics; D005786:Gene Expression Regulation; D005796:Genes; D006801:Humans; D007438:Introns / Q000235:genetics; D008562:Membrane Glycoproteins / Q000235:genetics; D051379:Mice; D023421:Models, Animal; D005810:Multigene Family; D016133:Polymerase Chain Reaction
D003598:Cytoskeletal Proteins; D018076:DNA, Complementary; C081560:DSC1 protein, human; C081561:DSC2 protein, human; C490356:DSC3 protein, human; D051187:Desmocollins; D051182:Desmogleins; D051180:Desmoplakins; C489910:Dsc1 protein, mouse; C489912:Dsc2 protein, mouse; D008562:Membrane Glycoproteins; C090171:Dsc3 protein, mouse