Cell migration from the corticostriatal angle to the basal telencephalon in rat embryos.
Lateral cortical stream (LCS) has been described as a flow of cell migration found in the lateral cortex of the embryonic telencephalon of mammals. The destinations of the cell migration were reported as the ventrolateral neocortex, the pyriform cortex, endopyriform nucleus and the claustrum. At the same time, however, other destinations of LCS have been suggested in the ventral telencephalon. Therefore, we investigated the additional destinations of the LCS using a combination of several molecular biological techniques. Using an expression vector of modified green fluorescent protein (GFP) introduced into the ventricular zone (VZ) around the corticostriatal angle, both tangential and radial cell migration was revealed in the LCS. The radial cell migration in the LCS supplied cells to the ventro-lateral neocortex, pyriform cortex and to the level of the lateral olfactory tract. In the second experiment, we injected COS-1 cells transfected with a Sema3A expression vector into one side of the neocortex. The cell supply to the destination of the LCS ceased due to the formation of a large necrosis in the LCS, which was triggered by the Sema3A-COS cell injection, and the dense cell layer in the olfactory tubercle shrunk on the side where COS cells were injected. These data indicated that the majority of neurons in the dense cell layer of the olfactory tubercle reached this point through the LCS. One of the origins of the cells in the LCS would be in the corticostriatal angle.
Department of Morphological Brain Science, Graduate School of Medicine, Kyoto University, Japan.
N
N
Tamamaki
K
K
Nakamura
T
T
Kaneko
D000818:Animals; D019556:COS Cells; D002352:Carrier Proteins / Q000235:genetics; D002465:Cell Movement / Q000502:physiology*; D002540:Cerebral Cortex / Q000166:cytology* / Q000196:embryology*; D003342:Corpus Striatum / Q000166:cytology / Q000196:embryology; D005260:Female; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D007202:Indicators and Reagents / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000235:genetics; D009457:Neuroglia / Q000166:cytology; D039942:Neuropilin-1; D009833:Olfactory Pathways / Q000166:cytology / Q000196:embryology; D011247:Pregnancy; D051381:Rats; D040121:Semaphorin-3A; D014162:Transfection
D002352:Carrier Proteins; D007202:Indicators and Reagents; D008164:Luminescent Proteins; D009419:Nerve Tissue Proteins; C488218:Sema3a protein, rat; D040121:Semaphorin-3A; D039942:Neuropilin-1; D049452:Green Fluorescent Proteins