Profiles of cytokine expression in radicular cyst-lining epithelium examined by RT-PCR.
Levels of messenger RNA (mRNA) extracted from five samples of radicular cyst-lining epithelium were analyzed for cytokines, growth factors and epithelial cell growth-related receptors by RT-PCR. All five samples expressed IL-1alpha, -1beta, IL-6, IL-8, IL-11, TGF-beta1, PDGF-A and aFGF, and receptors for EGF (c-erbB), KGF, HGF (c-met) and IL-6. Some of the specimens expressed MIP-1alpha, RANTES, GM-CSF, M-CSF, TNF-alpha, PDGF-B and bFGF, but no expression of IL-2, IL-4, IFN-gamma, IGF-I, EGF and KGF was detected. These results indicate that radicular cyst-lining epithelium, which is considered to be identical to the cell rests of Malassez, may play a role in periodontal pocket formation or apical cyst formation by interaction with surrounding connective tissue or hematopoietic cells through the expression of various cytokines.
Department of Biochemistry, Nihon University School of Dentistry, Tokyo, Japan.
M
M
Ohshima
T
T
Nishiyama
K
K
Tokunaga
S
S
Sato
M
M
Maeno
K
K
Otsuka
D016207:Cytokines / Q000096:biosynthesis*; D004847:Epithelial Cells / Q000378:metabolism*; D006133:Growth Substances / Q000096:biosynthesis*; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis; D011842:Radicular Cyst / Q000378:metabolism*; D017978:Receptors, Growth Factor / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D016207:Cytokines; D006133:Growth Substances; D012333:RNA, Messenger; D017978:Receptors, Growth Factor