Purification of the RelB and RelE proteins of Escherichia coli: RelE binds to RelB and to ribosomes.
The direct interaction of the Escherichia coli cytotoxin RelE with its specific antidote, RelB, was demonstrated in two ways: (i) copurification of the two proteins and (ii) a positive yeast two-hybrid assay involving the relB and relE genes. In addition, the purified RelE protein exhibited ribosome-binding activity in an in vitro assay, supporting previous observations suggesting that it is an inhibitor of translation.
Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia V8W 3P6, Canada.
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Galvani
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Terry
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Ishiguro
D000992:Antitoxins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D001427:Bacterial Toxins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism* / Q000633:toxicity; D003603:Cytotoxins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism / Q000633:toxicity; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*; D051841:Transcription Factor RelB; D014157:Transcription Factors / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D000992:Antitoxins; D001427:Bacterial Toxins; D003603:Cytotoxins; D011518:Proto-Oncogene Proteins; D014157:Transcription Factors; D051841:Transcription Factor RelB