The GemA protein of phage Mu and the GyrB gyrase subunit of Escherichia coli: the search for targets and interactions leading to the reversion of Mu-induced mutations.
The mutant bacteriophage Mugem2(Ts), known to synchronize the division of infected cells, to relax DNA supercoiling and, as prophage, to give rise to precisely excised revertants, has been thought to overexpress the gemA-mor operon, and genetic evidence suggests that the B subunit of DNA gyrase (GyrB) is the target of action of GemA. In two different double hybrid tests presented here, we find no evidence of GemA-GyrB protein-protein interaction. We do observe a GemA-GemA interaction, however, indicating that GemA can dimerize. In lacZ::Mu lysogens, overexpression of the gemA-mor operon from a plasmid, under control of the L-arabinose inducible p(araBAD) promoter, does not permit the recovery of Lac(+) revertants. These observations suggest that GyrB is not the direct target of GemA action and that the various phenotypes of Mugem2(Ts) are not caused by overexpression of the gemA-mor operon.
Abbes
C
C
Institut Jacques-Monod, CNRS, Université Paris 6, Université Paris 7, 75251 Paris cedex 05, France.
Joseleau-Petit
D
D
Liébart
J C
JC
D'Ari
R
R
Sezonov
G
G
D001426:Bacterial Proteins / Q000378:metabolism*; D010583:Bacteriophage mu / Q000235:genetics / Q000378:metabolism*; D027081:DNA Gyrase; D017931:DNA Primers / Q000737:chemistry; D004250:DNA Topoisomerases, Type II / Q000378:metabolism*; D019281:Dimerization; D004926:Escherichia coli / Q000201:enzymology*; D009154:Mutation; D009876:Operon; D010957:Plasmids; D011485:Protein Binding; D014161:Transduction, Genetic; D014764:Viral Proteins / Q000378:metabolism*
D001426:Bacterial Proteins; D017931:DNA Primers; D014764:Viral Proteins; D027081:DNA Gyrase; D004250:DNA Topoisomerases, Type II