Dichelobacter nodosus serotype M fimbrial subunit gene: implications for serological classification.
Dichelobacter nodosus fimbrial subunit gene (fimA) from a serotype M strain (M-SPAHL) was investigated in this study. A primer set targeting the relatively conserved fimA regions and based on the published sequence from Nepalese serogroup M isolates (Nepalese M), failed to amplify the fimA of M-SPAHL. However, when the downstream primer was substituted with a primer that is specific for other serogroups of D. nodosus, the fimA was successfully amplified. Cloning followed by DNA sequencing, revealed that the M-SPAHL fimA was different to the Nepalese M fimA. The predicted amino acid sequence of the M-SPAHL fimA did not show homology to any known serogroups or serotypes. The most similar sequence was from serotype F1, and not Nepalese M. The consequences of serological relatedness and sequence dissimilarity are discussed.
Zhou
H
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Animal and Food Sciences Division, Lincoln University, Canterbury, New Zealand.
Hickford
J G
JG
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D020376:Dichelobacter nodosus / Q000145:classification / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D036781:Fimbriae Proteins; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D001426:Bacterial Proteins; D004269:DNA, Bacterial; C063055:fimbrillin; D036781:Fimbriae Proteins