Decolourisation of synthetic and spentwash melanoidins using the white-rot fungus Phanerochaete chrysosporium JAG-40.
Phanerochaete chrysosporium JAG-40 was isolated from the soil samples saturated with spilled molasses collected from a sugar mill. This isolate decolourised synthetic and natural melanoidins present in spentwash in liquid fermentation; up to 80% in 6 days at 30 degrees C under aerobic conditions. A large inoculum size stimulated fungal biomass production, but this gave less decolourisation of pigment; 5% w/v (dry weight) mycelial suspension was found optimum for maximum decolourisation in melanoidin medium supplemented with glucose and peptone. Gel-filtration chromatography showed that larger molecular weight fractions of melanoidin were decolourised more rapidly than small molecular weight fractions.
Novopharm Fermentation, Winnipeg, Manitoba, Canada.
J
J
Dahiya
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D
Singh
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P
Nigam
D000434:Alcoholic Beverages; D001673:Biodegradation, Environmental; D002850:Chromatography, Gel; D004787:Environmental Pollution; D000431:Ethanol; D005285:Fermentation; D008965:Molasses; D020075:Phanerochaete / Q000302:isolation & purification / Q000378:metabolism*; D010860:Pigments, Biological / Q000378:metabolism; D011108:Polymers / Q000138:chemical synthesis / Q000378:metabolism*; D012988:Soil Microbiology; D018505:Waste Management
D010860:Pigments, Biological; D011108:Polymers; C011908:melanoidin polymers; D000431:Ethanol