Effect of batroxobin against dog heart ischemia/reperfusion injury.
OBJECTIVE
To study the effect of batroxobin(Bat) on dog heart ischemia/reperfusion (I/R) injury.


METHODS
Dog heart I/R injury was induced by occluding the left anterior descending coronary artery for 30 min and restoring blood perfusion for 90 min. Bat was intravenously injected before heart ischemia and 15 min before reperfusion. Plasma creatine kinase (CK), lactate dehydrogenase (LDH), and myocardial malondiaedehyde (MDA) concentrations were measured. The pathologic changes of I/R myocardium were observed.


RESULTS
Bat reduced the mortality rate of I/R dog (I/R group 65.0% vs Bat-I group 30.0% and Bat-II group 28.6%, P < 0.05). Myocytes of I/R heart showed intracellular edema, damaged mitochondria, and concentrated nucleus. Bat decreased these changes. In Bat-I and Bat-II group, plasma CK and LDH level were reduced, the +dp/dtmax and -dp/dtmax at 30 min after ischemia and 90 min after reperfusion were elevated, and left ventricular end dilation pressure (LVEDP) was lowered. The myocardial MDA contents were decreased by 42.3% and 38.1% (P < 0.01) in Bat-I and Bat-II group, respectively.


CONCLUSIONS
Bat may exert an apparent role against dog heart ischemia/reperfusion injury and improve myocardial function.
Institute of Cardiovascular Basic Research, Beijing Medical University, Beijing 100083, China. zsjiang@public.bta.net.cn
Z S
ZS
Jiang
C F
CF
Xia
Q P
QP
Tian
M G
MG
Fu
X H
XH
Wang
Y Z
YZ
Pang
C S
CS
Tang
N K
NK
Liu
D000818:Animals; D012103:Batroxobin / Q000494:pharmacology*; D003402:Creatine Kinase / Q000097:blood; D004285:Dogs; D005343:Fibrinolytic Agents / Q000494:pharmacology; D006334:Heart Function Tests; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D009206:Myocardium / Q000378:metabolism / Q000648:ultrastructure*
D005343:Fibrinolytic Agents; D008315:Malondialdehyde; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase; D012103:Batroxobin