Ellagic acid inhibited 2-aminofluorene and p-aminobenzoic acid acetylation by mononuclear leucocytes from Sprague-Dawley rats.
Following exposure of rats to the arylamine carcinogen 2-aminofluorene, DNA-carcinogen adducts were found in the liver and bladder target tissues, and also in circulating leucocytes. This work investigated the effect of ellagic acid on arylamine (2-aminofluorene and p-aminobenzoic acid) acetylations in rat leucocytes. Evidence is presented that rat mononuclear leucocytes are capable of acetylating 2-aminofluorene and p-aminobenzoic acid. Both lymphocytes and monocytes were able to acetylate arylamines during 18 h of culture. Cultured lymphocytes produced about twice as much N-acetyl-2-aminofluorene from 2-aminofluorene and 2.2-fold as much N-acetyl-p-aminobenzoic acid from p-aminobenzoic acid as monocytes. After cotreatment with ellagic acid the lymphocyte and monocyte cultures indicated that ellagic acid reduced 2-aminofluorene acetylation.
Ho
C C
CC
Department of Physiology, China Medical College, Taichung, Taiwan, Republic of China.
Tsai
H Y
HY
Lai
Y S
YS
Chung
J G
JG
D010129:4-Aminobenzoic Acid / Q000378:metabolism*; D000107:Acetylation / Q000187:drug effects; D000818:Animals; D001191:Arylamine N-Acetyltransferase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D004610:Ellagic Acid / Q000494:pharmacology*; D005449:Fluorenes / Q000378:metabolism*; D007962:Leukocytes / Q000378:metabolism*; D008214:Lymphocytes / Q000378:metabolism; D009000:Monocytes / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D005449:Fluorenes; D004610:Ellagic Acid; C012177:2-aminofluorene; D001191:Arylamine N-Acetyltransferase; D010129:4-Aminobenzoic Acid