Mechanisms for contractile effect of Dai-kenchu-to in isolated guinea pig ileum.
The mechanisms by which Dai-kenchu-to (TJ-100), a kampo medicine, enhances gastrointestinal motility was investigated using isolated guinea pig ileum. TJ-100 induced contractions accompanied by autonomous contraction at a concentration of more than 3 x 10(-4) g/ml in a dose-related manner. The TJ-100-induced ileal contraction was suppressed by atropine and tetrodotoxin, but not by hexamethonium. This effect was partially suppressed in the presence of high concentrations of ICS 205-930, a serotonin 4 (5-HT4) receptor antagonist. In addition, TJ-100 showed an acetylcholine (ACh)-releasing action in the smooth muscle tissues of ileum. These results suggest that contractile response induced by TJ-100 is partially mediated by ACh released from the cholinergic nerve endings and that 5-HT4 receptors would be involved in the effect of TJ-100.
Satoh
K
K
Kampo & Pharmacognosy Laboratory, R & D Division, Tsumura & Co, Yoshiwara, Ibaraki, Japan.
Hayakawa
T
T
Kase
Y
Y
Ishige
A
A
Sasaki
H
H
Nishikawa
S
S
Kurosawa
S
S
Yakabi
K
K
Nakamura
T
T
D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D005769:Gastrointestinal Motility / Q000187:drug effects*; D006168:Guinea Pigs; D018738:Hexamethonium / Q000494:pharmacology; D007082:Ileum / Q000187:drug effects*; D007211:Indoles / Q000494:pharmacology; D007418:Intestinal Pseudo-Obstruction / Q000188:drug therapy* / Q000503:physiopathology; D008297:Male; D020835:Medicine, Kampo; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects*; D018733:Nicotinic Antagonists / Q000494:pharmacology; D005894:Panax; D010276:Parasympatholytics / Q000494:pharmacology; D004364:Pharmaceutical Preparations; D010936:Plant Extracts / Q000037:antagonists & inhibitors / Q000494:pharmacology / Q000627:therapeutic use*; D012702:Serotonin Antagonists / Q000494:pharmacology; D013779:Tetrodotoxin / Q000494:pharmacology; D000077526:Tropisetron; D032106:Zanthoxylum; D030521:Zingiberaceae
D004365:Drugs, Chinese Herbal; D007211:Indoles; D018733:Nicotinic Antagonists; D010276:Parasympatholytics; D004364:Pharmaceutical Preparations; D010936:Plant Extracts; D012702:Serotonin Antagonists; C121414:dai-kenchu-to; D018738:Hexamethonium; D013779:Tetrodotoxin; D000077526:Tropisetron; D001285:Atropine; D000109:Acetylcholine