Cloning of a cDNA encoding EIN3-like protein (DC-EIL1) and decrease in its mRNA level during senescence in carnation flower tissues.
A cDNA clone encoding a putative EIN3-like protein (DC-EIL1) was obtained from total RNA isolated from senescing carnation (Dianthus caryophyllus L.) petals using RT-PCR and RACE techniques. The cDNA (2382 bp) contained an open reading frame of 1986 bp corresponding to 662 amino acids. The amino acid sequence of the N-terminal half of the protein, ranging from 80-300 amino acid residues, had 84% identity with that of the corresponding regions of Arabidopsis EIN3 and tobacco TEIL, although the overall identity was 49% and 52%, respectively. Northern blot analysis revealed that the amount of mRNA corresponding to DC-EIL1 decreased in flower tissues, especially in petals, during natural senescence and senescence induced by exogenously applied ethylene or ABA.
Waki
K
K
Laboratory of Bioadaptation, Graduate School of Agricultural Science, Tohoku University, Tsutsumidori-amamiyamachi 1-1, Aoba-ku, Sendai 981-8555, Japan.
Shibuya
K
K
Yoshioka
T
T
Hashiba
T
T
Satoh
S
S
D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins; D015152:Blotting, Northern; D016922:Cellular Senescence / Q000235:genetics / Q000502:physiology*; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000235:genetics; D004268:DNA-Binding Proteins; D019684:Magnoliopsida / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins; D018547:Plant Stems / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D018749:RNA, Plant / Q000378:metabolism; D016415:Sequence Alignment; D014157:Transcription Factors
D029681:Arabidopsis Proteins; D018744:DNA, Plant; D004268:DNA-Binding Proteins; C106987:EIN3 protein, Arabidopsis; D009687:Nuclear Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D014157:Transcription Factors