Oxidative/antioxidative effects of different lignin preparations on DNA in hamster V79 cells.
Endogenous oxidative damage to DNA is thought to be an important etiologic factor in the development of chronic diseases such as cancer. Many products of the vegetable kingdom have been suggested to limit oxidative damage to DNA in humans. To this group belong lignins, polyphenols present in all plants (including edible plants). The aim of this study was to examine oxidative/antioxidative effects of different lignin preparations on mammalian DNA. In addition to a water-soluble sulfur-free lignin 1 which was obtained by fractionation of hardwood hydrolysate, we investigated lignin 2 (obtained by oxidation of lignin 1), lignin 3 (prepared by the extraction of lignin 2 with a mixture ethanol-water 3:1), lignin 4 (Na-salt of lignin 3) and lignin 5 (prepared by extraction of lignin 2 with diethylether). Our results showed that only the original lignin 1 did not increase substantially the level of DNA damage. Lignins 2, 3, 4 and 5 increased both the level of frank DNA strand breaks + alkali-labile sites and the level of FPG-sensitive sites representing oxidative damage to DNA. Lignin 1 was further tested for its antioxidative activity against DNA base modifications generated by visible light+photosensitizer. Obtained results confirmed the oxygen species-scavenging activity of lignin 1.
Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Slovak Republic. exonslam@savba.sk
D
D
Slamenová
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B
Kosíková
J
J
Lábaj
L
L
Ruzeková
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002460:Cell Line; D004396:Coloring Agents / Q000494:pharmacology; D020552:Comet Assay; D006224:Cricetinae; D004247:DNA / Q000378:metabolism*; D004249:DNA Damage; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D008027:Light; D008031:Lignin / Q000378:metabolism*; D008751:Methylene Blue / Q000494:pharmacology; D018384:Oxidative Stress
D000975:Antioxidants; D004396:Coloring Agents; D008031:Lignin; D004247:DNA; D008751:Methylene Blue