Tracking Cryptosporidium parvum by sequence analysis of small double-stranded RNA.
We sequenced a 173-nucleotide fragment of the small double-stranded viruslike RNA of Cryptosporidium parvum isolates from 23 calves and 38 humans. Sequence diversity was detected at 17 sites. Isolates from the same outbreak had identical double-stranded RNA sequences, suggesting that this technique may be useful for tracking Cryptosporidium infection sources.
Xiao
L
L
Division of Parasitic Diseases, Centers for Disease Control and Prevention, Mail Stop F12, Atlanta, GA 30333, USA. lax0@cdc.gov
Limor
J
J
Bern
C
C
Lal
A A
AA
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D016785:Cryptosporidium parvum / Q000145:classification* / Q000235:genetics; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D012330:RNA, Double-Stranded / Q000737:chemistry*; D016053:RNA, Protozoan / Q000737:chemistry*
D012330:RNA, Double-Stranded; D016053:RNA, Protozoan