Molecular cloning and characterization of a novel mammalian endo-apyrase (LALP1).
Here we describe the cloning, localization, and characterization of a novel mammalian endo-apyrase (LALP1) in human and mouse. The predicted human LALP1 gene encodes a 604-amino acid protein, whereas the mouse Lalp1 gene encodes a 606-amino acid protein. The human and mouse genes have 88% amino acid sequence identity. These genes share considerable homologies with hLALP70, a recently discovered mammalian lysosomal endo-apyrase. The human LALP1 gene resides on chromosome 10q23-q24 and contains 12 exons and 11 introns covering a genomic region of approximately 46 kilobase pairs. The subcellular localization and enzymatic activity of LALP1 indicated that LALP1 is indeed an endo-apyrase with substrate preference for nucleoside triphosphates UTP, GTP, and CTP.
Department of Pathology, Immunology and Laboratory Medicine, Center for Mammalian Genetics and Diabetes Center of Excellence, College of Medicine, University of Florida, Gainesville, Florida 32610, USA.
J D
JD
Shi
T
T
Kukar
C Y
CY
Wang
Q Z
QZ
Li
P E
PE
Cruz
A
A
Davoodi-Semiromi
P
P
Yang
Y
Y
Gu
W
W
Lian
D H
DH
Wu
J X
JX
She
D000595:Amino Acid Sequence; D000818:Animals; D001081:Apyrase / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10; D003001:Cloning, Molecular; D003570:Cytidine Triphosphate / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008297:Male; D008322:Mammals; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011755:Pyrophosphatases / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D014162:Transfection; D014544:Uridine Triphosphate / Q000378:metabolism
D011994:Recombinant Proteins; D003570:Cytidine Triphosphate; D006160:Guanosine Triphosphate; D011755:Pyrophosphatases; D001081:Apyrase; C429540:ENTPD7 protein, human; C486105:Lysal2 protein, mouse; D014544:Uridine Triphosphate