Mechanism of suppression of cloned human suppressor T cells.
We report the mechanism of suppression of suppressor T cell clone III-1-C5 using helper T cell clone III-1-B6, mitogen responses and rIL-2. Clone III-1-C5 suppressed the mixed lymphocyte reaction (MLR) by secreting alloantigen non-specific, MHC non-restricted suppressor factor(s). Clone III-1-C5 did not suppress mitogen (PHA, Con A, PWM) response nor proliferation by exogeneous rIL-2. Clone III-1-C5 suppressed proliferation by clone III-1-B6, which augments proliferation by direct cell to cell contact with responder cells and not by soluble factors. These results indicated that suppressor T cells exhibit suppressive effects not only by inhibiting IL-2 synthesis but by inhibiting the direct effects of helper T-cells.
Second Department of Surgery, Hiroshima University School of Medicine, Japan.
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Yahata
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Fukada
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Hayamizu
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Okimoto
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Ishikawa
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Asahara
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Ono
K
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Dohi
D000818:Animals; D002999:Clone Cells; D003165:Complement System Proteins / Q000494:pharmacology; D016572:Cyclosporine / Q000494:pharmacology; D006801:Humans; D007108:Immune Tolerance; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed; D010835:Phytohemagglutinins; D011817:Rabbits; D011994:Recombinant Proteins / Q000494:pharmacology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000528:radiation effects; D013936:Thymidine / Q000378:metabolism; D014965:X-Rays
D007376:Interleukin-2; D010835:Phytohemagglutinins; D011994:Recombinant Proteins; D016572:Cyclosporine; D003165:Complement System Proteins; D013936:Thymidine