Tetrodotoxin-resistant conductivity and spinal effects of cutaneous C-fibre afferents in the rat.
The effect of the sodium channel blocking agent tetrodotoxin (TTX) on signal processing in afferent fibres of dorsal roots was tested in Sprague-Dawley rats. TTX applied to the dorsal roots L4-L6 blocked the fast afferent volleys from the sural nerve, which was stimulated electrically with supramaximal strength for A-fibres. Afferent C-fibre compound action potentials (CAPs) elicited by electrical stimulation of the dorsal root L5 peripherally from the TTX block or by electrical stimulation of the sural nerve likewise disappeared from the recording. Cord dorsum potentials (CDPs) recorded at the dorsal root entry zone of L4 were blocked completely if elicited by A-fibre volleys. In contrast, CDPs elicited by C-fibre stimulation persisted with longer latency and reduced amplitude in the first part of the CDP. During TTX block, C-fibre potentials could also be recorded from dorsal root filaments after stimulation of the sural nerve or the dorsal root L5 peripherally of the TTX-block. The results suggest that in the axonal membrane of cutaneous C-afferents, both TTX sensitive and TTX-resistant voltage gated sodium channels exist, the latter being responsible for the propagation of signals in a portion of C-fibres after TTX application. The TTX-resistant portion of the afferent potential does not seem to contribute much to the afferent C-fibre CAP before TTX application, but its central effects seem to be overproportionally strong.
Institut für Anatomie und Zellbiologie, Universität Heidelberg, Im Neuenheimer Feld 307, D-69120, Heidelberg, Germany. heinz.steffens@urz.uni-heidelberg.de
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Steffens
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Hoheisel
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Eek
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Mense
D000200:Action Potentials / Q000187:drug effects* / Q000502:physiology; D000818:Animals; D004305:Dose-Response Relationship, Drug; D008297:Male; D009412:Nerve Fibers / Q000187:drug effects* / Q000502:physiology; D009413:Nerve Fibers, Myelinated / Q000187:drug effects / Q000502:physiology; D009431:Neural Conduction / Q000187:drug effects* / Q000502:physiology; D009475:Neurons, Afferent / Q000187:drug effects* / Q000502:physiology; D009619:Nociceptors / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011930:Reaction Time / Q000187:drug effects / Q000502:physiology; D012867:Skin / Q000294:innervation; D015222:Sodium Channels / Q000187:drug effects / Q000378:metabolism; D013116:Spinal Cord / Q000187:drug effects* / Q000502:physiology; D013126:Spinal Nerve Roots / Q000187:drug effects* / Q000502:physiology; D013497:Sural Nerve / Q000187:drug effects / Q000502:physiology; D013779:Tetrodotoxin / Q000494:pharmacology*
D015222:Sodium Channels; D013779:Tetrodotoxin