Identification and quantification of base and nucleoside markers in extracts of Ganoderma lucidum, Ganoderma japonicum and Ganoderma capsules by micellar electrokinetic chromatography.
The present paper describes the development of a micellar electrokinetic chromatographic method for the determination of nucleoside (adenosine, uridine) and base (uracil) markers in aqueous extracts of Ganoderma medicinal preparations. The markers were successfully separated within 10 min using an 80 mM borate buffer, with 25 mM sodium dodecyl sulfate adjusted to pH 9.0, an operating voltage of 22 kV, temperature of 20 degrees C and a hydrodynamic injection time of 5 s. Separations were carried out in a fused-silica capillary with peak detection by direct UV at 254 nm. Following semi-validation of the method, with each analyte showing a good linear relationship over a 0.2 to 20 ppm concentration range (correlation coefficients from 0.9986 to 0.9998), the amounts of the three markers in the various forms of Ganoderma were easily determined using a relatively simple extraction procedure.
Department of Biology and Chemistry, City University of Hong Kong, Kowloon Tong, Hong Kong. bhhonyun@cityu.edu.hk
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D002021:Buffers; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009705:Nucleosides / Q000737:chemistry / Q000302:isolation & purification*; D020940:Reishi; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
D002021:Buffers; D004365:Drugs, Chinese Herbal; D009705:Nucleosides