Double lesions are produced in DNA oligomer by ionizing radiation and by metal-catalyzed H2O2 reactions.
It was demonstrated previously that double lesions are produced in DNA by ionizing radiation. These double lesions consist of adjacent nucleotides each bearing a modified base. The goal of the present investigation was to determine whether Fenton chemistry can generate the same kind of lesions. DNA oligomers were exposed to metal-catalyzed H(2)O(2) reactions, and the products were characterized by chromatography and by mass spectrometry. Double lesions are produced by this treatment in which deoxyguanosine is oxidized to 8-oxo-7,8-dihydrodeoxyguanosine and an adjacent pyrimidine nucleoside is degraded to a formamido remnant.
Patrzyc
H B
HB
Department of Molecular and Cellular Biophysics, Roswell Park Cancer Institute, Carlton and Elm Streets, Buffalo, NY 14263, USA.
Dawidzik
J B
JB
Budzinski
E E
EE
Iijima
H
H
Box
H C
HC
D000080242:8-Hydroxy-2'-Deoxyguanosine; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D003300:Copper / Q000494:pharmacology; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000528:radiation effects; D005609:Free Radicals; D006861:Hydrogen Peroxide / Q000494:pharmacology / Q000633:toxicity*; D007501:Iron / Q000494:pharmacology; D013058:Mass Spectrometry; D009571:Nitrilotriacetic Acid / Q000494:pharmacology; D009838:Oligodeoxyribonucleotides / Q000528:radiation effects*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011743:Pyrimidines / Q000528:radiation effects; D017382:Reactive Oxygen Species
C045076:Fenton's reagent; D005609:Free Radicals; D009838:Oligodeoxyribonucleotides; D011743:Pyrimidines; D017382:Reactive Oxygen Species; D003300:Copper; D000080242:8-Hydroxy-2'-Deoxyguanosine; D006861:Hydrogen Peroxide; D007501:Iron; D003849:Deoxyguanosine; D009571:Nitrilotriacetic Acid