Isolation, characterization, and mapping of a novel human KRAB zinc finger protein encoding gene ZNF463.
A novel human KRAB (Krüppel associated box) type zinc finger protein encoding gene, ZNF463, was obtained by mRNA differential display and RACE. It consists of 1904 nucleotides and encodes a protein of 463 amino acids with an amino-terminal KRAB domain and 12 carboxy-terminal C2H2 zinc finger units. The gene is mapped to chromosome 19q13.3 approximately 4 by FISH. As from Northern blot analysis ZNF463 is only expressed in testis, RT-PCR indicates that ZNF463 is expressed more highly in normal fertile adults than in fetus and azoospermic patients suggesting that it may play a role in human spermatogenesis.
Wu
H
H
Department of Medical Genetics, First Affiliated Hospital, West China University of Medical Sciences, Chengdu, Sichuan 610041, PR China.
Zhang
S
S
Qiu
W
W
Zhang
G
G
Xia
Q
Q
Xiao
C
C
Huang
X
X
Huang
M
M
Agen
P
P
Fan
T
T
Yang
J
J
Milunsky
A
A
D000328:Adult; D000595:Amino Acid Sequence; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods; D002888:Chromosomes, Human, Pair 19; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D020869:Gene Expression Profiling; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008297:Male; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D009845:Oligospermia / Q000235:genetics; D013091:Spermatogenesis / Q000235:genetics*; D013737:Testis / Q000378:metabolism* / Q000473:pathology; D016335:Zinc Fingers
D002352:Carrier Proteins; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D009363:Neoplasm Proteins; C403666:ZNF331 protein, human