Molecular cloning and characterization of a cDNA for a novel ethylene receptor, NT-ERS1, of tobacco (Nicotiana tabacum L.).
The cDNA encoding a novel member (NT-ERS1) of ethylene receptor family of tobacco (Nicotiana tabacum L.) was obtained by a combination of RT-PCR and 5'-/3'-RACE cloning. The cDNA was 2,092 nucleotides long and had an open reading frame of 1,911 bp encoding 637 amino acids. The deduced polypeptide lacked a response regulator domain, indicating that the ethylene receptor belongs to an ERS-group. The amino acid sequence was similar to respective members of the tobacco ethylene receptor family: 67.8% to NT-ETR1, 39.1% to NTHK1 and 31.1% to NTHK2. Comparison of amino acid sequence suggested that NT-ERS1 is the counterpart of Nr in the ethylene receptor family of tomato, which belongs to Solanaceae as does tobacco. Northern blot analysis showed that mRNA of NT-ERS1 was present in leaf, shoot and root tissues, and accumulated in leaves treated with exogenous ethylene. A mutated NT-ERS1 cDNA transgene, obtained by introducing one nucleotide substitution into NT-ETR1 cDNA, conferred ethylene insensitivity in tobacco plants, indicating that the translation product of the cDNA actually functioned in the plants.
Laboratory of Bio-adaptation, Graduate School of Agricultural Sciences, Tohoku University, Tsutsumidori-amamiyamachi 1-1, Aoba-ku, Sendai, 981-8555 Japan.
Y
Y
Terajima
H
H
Nukui
A
A
Kobayashi
S
S
Fujimoto
S
S
Hase
T
T
Yoshioka
T
T
Hashiba
S
S
Satoh
D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005030:Ethylenes / Q000378:metabolism*; D016296:Mutagenesis; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D012333:RNA, Messenger; D018749:RNA, Plant; D011956:Receptors, Cell Surface / Q000235:genetics*; D012639:Seeds; D014026:Nicotiana / Q000235:genetics*; D014170:Transformation, Genetic; D019076:Transgenes
D018076:DNA, Complementary; D005030:Ethylenes; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D011956:Receptors, Cell Surface; C083750:ethylene receptors, plant