Isolation and preliminary characterization of the medium-chain fatty acid:CoA ligase responsible for activation of short- and medium-chain fatty acids in colonic mucosa from swine.
The ATP-dependent activation of short- and medium-chain fatty acids to their respective CoA thioester adducts was investigated in the colonic mucosa from swine. Subcellular fractionation of a homogenate of the mucosa from the entire length of the colon revealed a predominantly mitochondrial localization for activity toward fatty acids ranging from propionate through laurate. These activities could be released from mitochondria in soluble form by freeze-thaw lysis. Purification of these activities revealed that they all appeared to reside with a single enzyme. This suggests that the entire colon contains a single form of medium-chain fatty acid:CoA ligase (MCFA:CoA ligase). The ligase also had activity toward benzoate and salicylate, although this activity was significantly lower than activity toward medium-chain fatty acids. The enzyme had the highest activity at Vmax with butyrate as substrate and had the lowest Km for octanoate. Butyrate and octanoate were mutually inhibitory. Activity toward both substrates was also efficiently inhibited by cyclohexane carboxylate. The molecular weight of the enzyme was estimated by gel filtration chromatography to be ca. 46,500. These data indicate that the colonic MCFA:CoA ligase is significantly different from the hepatic and kidney MCFA:CoA ligases.
Department of Veterans Affairs Medical Center, San Francisco, California 94121, USA.
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Vessey
D000106:Acetate-CoA Ligase / Q000737:chemistry / Q000302:isolation & purification* / Q000502:physiology*; D000818:Animals; D002458:Cell Fractionation; D002850:Chromatography, Gel; D003106:Colon / Q000378:metabolism*; D005232:Fatty Acids, Volatile / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism*; D008928:Mitochondria; D008970:Molecular Weight; D051381:Rats; D013379:Substrate Specificity; D013552:Swine
D005232:Fatty Acids, Volatile; D000106:Acetate-CoA Ligase