SHV-27, a novel cefotaxime-hydrolysing beta-lactamase, identified in Klebsiella pneumoniae isolates from a Brazilian hospital.
From a collection of cefotaxime-resistant Klebsiella pneumoniae isolated from neonatal blood culture specimens in a maternity hospital in Aracaju, Brazil, two isolates (strains KPBRZ-842 and -843, indistinguishable by pulsed-field gel electrophoresis) were found to produce beta-lactamases with isoelectric points (pI) of 5.4 and 8.2, respectively. Using a gel overlay method, cefotaxime hydrolysis was shown to be associated with the pI 8.2 protein. Nucleotide sequencing of the gene encoding the pI 8.2 beta-lactamase revealed a bla(SHV-ESBL)-type gene differing from the gene encoding SHV-1 by three silent point mutations, and a fourth that resulted in an amino acid substitution, aspartate for glycine, at position 156. This novel SHV-type extended-spectrum beta-lactamase is designated SHV-27.
Royal Liverpool University Hospital, Department of Medical Microbiology, Liverpool L7 8XP, UK. jecmm@liverpool.ac.uk
J E
JE
Corkill
L E
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Cuevas
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Gurgel
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J
Greensill
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CA
Hart
D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000494:pharmacology; D001398:Aztreonam / Q000494:pharmacology; D001938:Brazil; D002439:Cefotaxime / Q000378:metabolism* / Q000494:pharmacology; D002442:Ceftazidime / Q000494:pharmacology; D004252:DNA Mutational Analysis; D004352:Drug Resistance, Microbial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D006761:Hospitals; D006801:Humans; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D017354:Point Mutation / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D001618:beta-Lactamases / Q000302:isolation & purification / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D002442:Ceftazidime; C430824:beta-lactamase SHV-27; D001618:beta-Lactamases; D001398:Aztreonam; D002439:Cefotaxime