New method for high-performance liquid chromatographic separation and fluorescence detection of ginsenosides.
A novel pre-column derivatization method for the quantitative determination of ginsenosides by HPLC with fluorescence detection was established. The double bond at the C24-C25 position of ginsenoside was converted into an aldehyde group by means of ozonolysis. Then the aldehyde group reacts with FMOC-hydrazine forming the ginsenoside FMOC-hydrazone. The derivatized products were separated by RP-HPLC with gradient elution. The detection limits of ginsenosides Rg1 and Rb1 were 2.0 ng (about 2.5 pmol) and 1.0 ng (about 0.9 pmol), respectively. This method can be used for all ginsenosides having the C24-C25 double bond.
Shangguan
D
D
Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing.
Han
H
H
Zhao
R
R
Zhao
Y
Y
Xiong
S
S
Liu
G
G
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D036145:Ginsenosides; D012044:Regression Analysis; D012503:Saponins / Q000032:analysis / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
D036145:Ginsenosides; D012503:Saponins