Distribution and prognostic value of the fibroblast growth factor-2 low-affinity binding sites in human breast cancer.
We performed a competitive binding study with 125I-labelled FGF (fibroblast growth factor)-2 and unlabelled FGF-2 in an unselected series of two hundred and thirty human primary breast cancers. One hundred and ninety-two breast cancer biopsies possessed FGF-2 low-affinity binding sites (FGF-2 LABS). The median dissociation constant was 2.4 nM (range, 1.03-18) and the median concentration of membrane protein was 6187.5 fmol/mg (range, 831-90,000). FGF-2 LABS concentrations were positively correlated to the progesterone receptor level. Cox univariate analyses showed that the FGF-2 LABS (> or = upper quartile) was associated to a longer overall survival (p = 0.05; RR = 0.042); node involvement, estrogen receptor progesterone receptor and histoprognostic grading were also prognostic. In Cox multivariate analyses, only the progesterone receptor, estrogen receptor, node involvement and FGF-2 LABS were prognostic factors; the FGF-2 LABS were associated with a longer overall survival (p = 0.033; RR = 0.068). The present study showed that FGF-2 LABS have only a limited role as a prognostic factor in breast cancer.
Laboratoire d'Oncologie Moléculaire Humaine, Centre Oscar Lambert, rue F. Combemale, B.P. 307, 59020 Lille, France.
V D
VD
Blanckaert
L
L
Hornez
M
M
Hebbar
M M
MM
Louchez
H
H
Hondermarck
J P
JP
Peyrat
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000704:Analysis of Variance; D001665:Binding Sites; D001706:Biopsy; D001943:Breast Neoplasms / Q000401:mortality / Q000473:pathology* / Q000601:surgery; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D006801:Humans; D007457:Iodine Radioisotopes; D007700:Kinetics; D008207:Lymphatic Metastasis; D008875:Middle Aged; D011379:Prognosis; D011869:Radioligand Assay; D020794:Receptor Protein-Tyrosine Kinases / Q000032:analysis / Q000378:metabolism*; D051497:Receptor, Fibroblast Growth Factor, Type 2; D011960:Receptors, Estrogen / Q000032:analysis; D017468:Receptors, Fibroblast Growth Factor / Q000032:analysis / Q000378:metabolism*; D011980:Receptors, Progesterone / Q000032:analysis; D012189:Retrospective Studies
D007457:Iodine Radioisotopes; D011960:Receptors, Estrogen; D017468:Receptors, Fibroblast Growth Factor; D011980:Receptors, Progesterone; D016222:Fibroblast Growth Factor 2; C496353:FGFR2 protein, human; D020794:Receptor Protein-Tyrosine Kinases; D051497:Receptor, Fibroblast Growth Factor, Type 2