Malignant transformation by the DNA-protein crosslinking agent trans-Pt(II) diamminedichloride.
Trans-Pt(II) diamminedichloride (trans-Pt) produced high levels of DNA-protein crosslinks in mouse C3H-10T1/2 cells and mouse 3T3 cells. Trans-Pt induced malignant transformation in these cell types at all doses tested. In 10T1/2 cells trans-Pt induced moderate levels of transformation similar to that induced by equitoxic doses of X-rays. Trans-Pt did not act as a tumor promoter and enhance the transformation frequency seen with X-rays when cells were irradiated and then treated with this agent.
Harvard School of Public Health, Boston, MA 02115, USA.
A J
AJ
Fornace
J B
JB
Little
D016475:3T3 Cells / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced* / Q000528:radiation effects; D002945:Cisplatin / Q000633:toxicity*; D003432:Cross-Linking Reagents / Q000633:toxicity*; D016895:Culture Media, Serum-Free; D004247:DNA / Q000187:drug effects; D018736:DNA Adducts; D004249:DNA Damage; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D007939:Leukemia L1210 / Q000473:pathology; D051379:Mice; D008809:Mice, Inbred C3H; D011506:Proteins / Q000187:drug effects; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects
D003432:Cross-Linking Reagents; D016895:Culture Media, Serum-Free; D018736:DNA Adducts; D011506:Proteins; C034697:transplatin; D004247:DNA; D002945:Cisplatin