Isolation of a gene encoding a putative polyamine transporter from Candida albicans, GPT1.
A gene encoding a transport protein from the pathogenic yeast, Candida albicans, has been isolated during a complementation experiment utilizing an ornithine decarboxylase-negative (spe1 Delta) strain of Saccharomyces cerevisiae. This gene restores gamma-aminobutyric acid (GABA) transport to a GABA transport-negative mutant of S. cerevisiae and encodes a protein which putatively allows transport of one or more of the polyamines. We have assigned the name GPT1 (GABA/polyamine transporter) to this gene.
McNemar
M D
MD
Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, PA 19140, USA.
Gorman
J A
JA
Buckley
H R
HR
D000595:Amino Acid Sequence; D001483:Base Sequence; D002176:Candida albicans / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D005656:Fungal Proteins; D050485:GABA Plasma Membrane Transport Proteins; D015723:Gene Library; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D027361:Organic Anion Transporters; D009955:Ornithine Decarboxylase / Q000235:genetics / Q000378:metabolism; D011073:Polyamines / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D005680:gamma-Aminobutyric Acid / Q000502:physiology
D002352:Carrier Proteins; D005656:Fungal Proteins; D050485:GABA Plasma Membrane Transport Proteins; C433885:GPT1 protein, Candida albicans; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D027361:Organic Anion Transporters; D011073:Polyamines; D029701:Saccharomyces cerevisiae Proteins; C513599:UGA4 protein, S cerevisiae; D005680:gamma-Aminobutyric Acid; D009955:Ornithine Decarboxylase