Molecular and cell biology of skin wound healing in a pig model.
To define the pattern of change at the molecular and cellular levels during the healing of excisional skin wounds in the skeletally immature pig, mRNA levels for relevant molecules were assessed by semiquantitative RT-PCR using porcine specific primer sets and RNA isolated from normal skin and samples at various time post-wounding. Analysis of cellular change was assessed by DNA quantification and histology of tissue sections. The results demonstrated that the changes in the pattern of RNA and DNA content of the scar tissue were consistent with the observed increasing cellularity. The mRNA levels for collagen I, III, HSP47, IL-1, TGF-beta, MMP-1, -2 and -9, TIMP-1, -2, and-4, PAI-1, versican were significantly elevated during healing; levels for biglycan and fibromodulin were not significantly altered; and the mRNA levels for TIMP-3 were depressed. These findings suggest that skin wound healing is a series of complex matrix-cell interactions that involve cellular migration and inflammation, followed by proliferation of fibroblasts with new collagen synthesis, and lastly tissue remodeling of the scar.
McCaig Center for Joint Injury and Arthritis Research, Department of Microbiology and Infectious Disease, Faculty of Medicine, University of Calgary, Alberta, Canada T2N 4N1.
J F
JF
Wang
M E
ME
Olson
C R
CR
Reno
W
W
Kulyk
J B
JB
Wright
D A
DA
Hart
D000818:Animals; D003094:Collagen / Q000235:genetics; D004247:DNA / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression; D050958:HSP47 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000235:genetics; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics; D008666:Metalloendopeptidases / Q000235:genetics; D011509:Proteoglycans / Q000235:genetics; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012867:Skin / Q000166:cytology / Q000276:immunology / Q000293:injuries / Q000378:metabolism*; D013552:Swine; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000235:genetics; D014945:Wound Healing / Q000276:immunology / Q000502:physiology*
D050958:HSP47 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D007375:Interleukin-1; D011509:Proteoglycans; D012333:RNA, Messenger; C494660:SERPINH1 protein, human; D019715:Tissue Inhibitor of Metalloproteinase-1; D016212:Transforming Growth Factor beta; D012313:RNA; D003094:Collagen; D004247:DNA; D008666:Metalloendopeptidases