Qualitative gene profiling: a novel tool in genomics and in pharmacogenomics that deciphers messenger RNA isoforms diversity.
RNA splicing is a tightly regulated process. It is essential for gene expression and, therefore, intervenes in every biological phenomenon in mammals. RNA splicing regulation is cell type-specific in such a way that a cellular situation can be characterised by its repertoire of spliced events, the spliceome. Comparison of the splicing repertoire of two situations identifies alternative exons and introns. This regulation involves cis-acting sequences and transacting factors. Mutations, as well as modifications of signalling pathways, can alter the accuracy of splicing. Since deletion of exons or retention of introns within coding sequences modifies the corresponding proteins and functional domains of proteins are encoded by contiguous exons, identifying changes in the spliceome pinpoints functional domains, which are specifically regulated at the level of RNA splicing. We have developed a new method of gene profiling, qualitative gene profiling, that allows the comparative study of the repertoires of spliced events that characterise distinct physiopathological situations. We present in this review the different uses of this new genomic technique that can help each step of the R&D process in the pharmaceutical industry, and that represents a short cut towards functional genomics and pharmacogenomics.
Schweighoffer
F
F
ExonHit Therapeutics SA, Paris, France. fabien.schweighoffer@exonhit.com
Ait-Ikhlef
A
A
Resink
A L
AL
Brinkman
B
B
Melle-Milovanovic
D
D
Laurent-Puig
P
P
Kearsey
J
J
Bracco
L
L
D000818:Animals; D006801:Humans; D008967:Molecular Biology / Q000379:methods*; D010597:Pharmacogenetics / Q000379:methods*; D020033:Protein Isoforms; D012326:RNA Splicing; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics*
D020033:Protein Isoforms; D012333:RNA, Messenger