Peptidomics of the pars intercerebralis-corpus cardiacum complex of the migratory locust, Locusta migratoria.
The pars intercerebralis-corpora cardiaca system (PI-CC) of insects is the endocrinological equivalent of the hypothalamus-pituitary system of vertebrates. Peptide profiles of the pars intercerebralis and the corpora cardiaca were characterized using simple sampling protocols in combination with MALDI-TOF and electrospray ionization double quadrupole time of flight (ESI-Qq-TOF) mass spectrometric technologies. The results were compared with earlier results of conventional sequencing methods and immunocytochemical methods. In addition to many known peptides, several m/z signals corresponding to putative novel peptides were observed in the corpora cardiaca and/or pars intercerebralis. Furthermore, for a number of peptides evidence was provided about their localization and MALDI-TOF analysis of the released material from the corpora cardiaca yielded information on the hormonal status of particular brain peptides.
Zoological Institute, Catholic University Leuven, Belgium. Elke.Clynen@bio.kuleven.ac.be
E
E
Clynen
G
G
Baggerman
D
D
Veelaert
A
A
Cerstiaens
D
D
Van der Horst
L
L
Harthoorn
R
R
Derua
E
E
Waelkens
A
A
De Loof
L
L
Schoofs
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D006110:Grasshoppers / Q000502:physiology*; D007030:Hypothalamo-Hypophyseal System / Q000502:physiology; D019476:Insect Proteins / Q000737:chemistry*; D018716:Molecular Mimicry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000662:veterinary
D019476:Insect Proteins