A simple isolation method for the major catechins in green tea using high-speed countercurrent chromatography.
An efficient protocol for the preparative purification of the major tea catechins (-)-epicatechin gallate (3) and epigallocatechin gallate (5) has been developed, employing liquid-liquid partitioning, high-speed countercurrent chromatography, and gel chromatography for final purification. The method is suitable for scale-up to significantly larger quantities.
Baumann
D
D
Institut für Pharmazie, Friedrich-Schiller-Universität Jena, Semmelweisstrasse 10, D-07743 Jena, Germany.
Adler
S
S
Hamburger
M
M
D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D013662:Tea / Q000737:chemistry*
D013662:Tea; D002392:Catechin; C057580:gallocatechol