NRG1 is required for glucose repression of the SUC2 and GAL genes of Saccharomyces cerevisiae.
BACKGROUND
Glucose repression of transcription in the yeast, Saccharomyces cerevisiae, has been shown to be controlled by several factors, including two repressors called Mig1 and Mig2. Past results suggest that other repressors may be involved in glucose repression.


RESULTS
By a screen for factors that control transcription of the glucose-repressible SUC2 gene of S. cerevisiae, the NRG1 gene was identified. Analysis of an nrg1Delta mutant has demonstrated that mRNA levels are elevated at both the SUC2 and of the GAL genes of S. cerevisiae when cells are grown under normally glucose-repressing conditions. In addition, genetic interactions have been detected between nrg1Delta and other factors that control SUC2 transcription.


CONCLUSIONS
The analysis of nrg1Delta demonstrates that Nrg1 plays a role in glucose repression of the SUC2 and GAL genes of S. cerevisiae. Thus, three repressors, Nrg1, Mig1, and Mig2, are involved as the downstream targets of the glucose signaling in S. cerevisiae.
Zhou
H
H
Department of Genetics Harvard Medical School 200 Longwood Avenue Boston, MA 02115, USA. hzhou_2000@yahoo.com
Winston
F
F
D000251:Adenosine Triphosphatases; D004268:DNA-Binding Proteins / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D016153:Genes, Suppressor; D005947:Glucose / Q000494:pharmacology*; D006026:Glycoside Hydrolases / Q000096:biosynthesis / Q000235:genetics*; D009154:Mutation; D009687:Nuclear Proteins; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D012331:RNA, Fungal / Q000096:biosynthesis; D012097:Repressor Proteins / Q000235:genetics / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic; D043324:beta-Fructofuranosidase
D004268:DNA-Binding Proteins; C085976:MIG1 protein, S cerevisiae; C117881:NRG1 protein, S cerevisiae; D009687:Nuclear Proteins; D012331:RNA, Fungal; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C071570:SNF1-related protein kinases; D017346:Protein Serine-Threonine Kinases; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase; D000251:Adenosine Triphosphatases; C499333:SNF2 protein, S cerevisiae; D005947:Glucose