Experimental small bowel transplantation using newborn intestine in rats: III. Long-term cryopreservation of rat newborn intestine.
BACKGROUND
If long-term organ cryopreservation can be attained, a significant achievement will have been made to address the problem for donor shortage. Fetal intestine has been known to revascularize naturally without vascular anastmosis. The authors have confirmed previously that the newborn intestine also could develop to maturity in the host omentum. Here, the authors examined whether the cryopreserved newborn intestine could revascularize in the syngeneic combination using the 2 different solutions and whether cryopreservation affect their antigenicity in the allogeneic combination.


METHODS
Inbred rat strains of LEW (MHC haplotype; RT1(l)) and BN (RT1(n)) were used. LEW newborn intestinal grafts were stored in RPMI-1640 or University of Wisconsin solution with 10% DEMSO (n = 10 in each group). The grafts were placed into a cold (4 degrees C) preservation solution for 30 minutes and then placed into a freezing chamber and cooled to -80 degrees C at -1 degrees C/min after 12 hours quenched to -180 degrees C in liquid nitrogen for longer than 30 days. Then, the cryopreserved grafts under the 2 different solutions were transplanted syngenicaly (LEW to LEW). The cryopreserved BN grafts also were implanted into the LEW omentum pouch. The allotransplantation was received with a 14-day high-dose course of tacrolimus (0.64 mg/kg, intramuscularly). The grafts were evaluated histologically at 4 weeks after transplantation. Fresh newborn intestines implanted in this syngeneic and allogeneic combination were evaluated as each control group.


RESULTS
In the syngeneic combination, more than 90% of the mature intestine were obtained. There was no significant difference among the different solution and the fresh group. However, in the allogeneic combination, both fresh and cryopreserved grafts were histologically poor.


CONCLUSIONS
This is the first report showing that long-term cryopreservation was not harmful for neovascularization of newborn intestine. Long-term cryopreservation did not reduce the antigenicity of the newborn intestine. J Pediatr Surg 36:602-604.
Tahara
K
K
Division of Organ Replacement Research, Center for Molecular Medicine, Jichi Medical School, 3-1-1, Yakushiji, Minamikawachi, Kawachi, Tochigi 332-0498, Japan.
Uchida
H
H
Kawarasaki
H
H
Hasizume
K
K
Kobayashi
E
E
D000818:Animals; D000831:Animals, Newborn; D015925:Cryopreservation / Q000379:methods*; D005260:Female; D006084:Graft Rejection; D006085:Graft Survival; D007421:Intestine, Small / Q000473:pathology* / Q000637:transplantation*; D008297:Male; D023421:Models, Animal; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D016377:Organ Transplantation / Q000379:methods*; D051381:Rats; D011917:Rats, Inbred Lew; D012016:Reference Values; D012680:Sensitivity and Specificity; D016559:Tacrolimus / Q000494:pharmacology*
D019188:Organ Preservation Solutions; D016559:Tacrolimus