The role of copper in topa quinone biogenesis and catalysis, as probed by azide inhibition of a copper amine oxidase from yeast.
All known copper amine oxidases (CAOs) contain 2,4,5-trihydroxyphenylalanine quinone (TPQ) as a redox cofactor. TPQ is derived posttranslationally from a specific tyrosine residue within the protein itself, and is utilized by the enzyme to oxidize amines to aldehydes. Several oxidative mechanisms for both turnover and the biogenesis of the cofactor have been proposed in recent years, which differ mainly in the nature of the interaction of oxygen with the enzyme. In this study, azide is used to probe the role of copper in catalysis and biogenesis, especially with respect to potential interactions between the metal and oxygen. During turnover, it is found that azide is a noncompetitive inhibitor with respect to O(2), most consistent mechanistically with oxygen binding off the metal prior to reaction. During biogenesis, it is found that azide likely prohibits ligation of the precursor tyrosine to the copper, thus preventing the formation of this key intermediate. This result is consistent with previous proposals, where the copper-tyrosine unit is the species that undergoes reaction with O(2). In addition, it is found that oxygen consumption is kinetically uncoupled from TPQ formation; this leads to an expanded kinetic model for biogenesis, with important implications for previous results.
Schwartz
B
B
Departments of Chemistry and of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA.
Olgin
A K
AK
Klinman
J P
JP
D006631:Amine Oxidase (Copper-Containing) / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000693:Anaerobiosis; D002384:Catalysis; D003067:Coenzymes / Q000096:biosynthesis; D003300:Copper / Q000737:chemistry*; D004295:Dihydroxyphenylalanine / Q000031:analogs & derivatives / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000737:chemistry*; D004792:Enzyme Precursors / Q000378:metabolism; D020035:Holoenzymes / Q000378:metabolism; D007700:Kinetics; D010100:Oxygen / Q000037:antagonists & inhibitors / Q000378:metabolism; D010101:Oxygen Consumption; D010843:Pichia / Q000201:enzymology; D019810:Sodium Azide / Q000737:chemistry*; D017186:Titrimetry
C086758:2,4,5-trihydroxyphenylalanine quinone; D003067:Coenzymes; D004791:Enzyme Inhibitors; D004792:Enzyme Precursors; D020035:Holoenzymes; D004295:Dihydroxyphenylalanine; C067626:6-hydroxydopa quinone; D003300:Copper; D019810:Sodium Azide; D006631:Amine Oxidase (Copper-Containing); D010100:Oxygen