Mechanism of the photocatalytic inactivation of Lactobacillus casei phage PL-1 by titania thin film.
The mechanism of the inactivation of Lactobacillus casei phage PL-1 suspended in a phosphate buffer by black-light (BL) -catalytic titanium dioxide (TiO2) thin film was studied. Generation of both superoxide anions (O2-) and hydroxyl radicals (*OH) was confirmed in the aqueous medium in which TiO2 film was settled with BL irradiation under gentle shaking. With BL-irradiation alone without TiO2 film, only O2- was generated to some extent. The genome DNA inside the phage particles was found to be fragmented by the treatment of PL-1 phages with BL-catalytic TiO2 film. The phage inactivation by BL-catalytic TiO2 film was inhibited by the addition of albumin in a concentration-dependent manner. BL-catalytic TiO2 film was considered to cause primarily the damage to the capsid protein through the generation of active oxygen species such as *OH, followed by damage to the genome DNA inside the phage particles.
Kashige
N
N
Faculty of Pharmaceutical Sciences, Fukuoka University, Japan. kashige@fukuoka-u.ac.jp
Kakita
Y
Y
Nakashima
Y
Y
Miake
F
F
Watanabe
K
K
D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D002213:Capsid / Q000528:radiation effects; D002384:Catalysis; D004249:DNA Damage; D004279:DNA, Viral / Q000378:metabolism / Q000528:radiation effects; D004587:Electrophoresis, Agar Gel; D017665:Hydroxyl Radical / Q000378:metabolism; D007780:Lacticaseibacillus casei / Q000821:virology*; D010083:Oxidants, Photochemical; D010084:Oxidation-Reduction; D010777:Photochemistry; D017382:Reactive Oxygen Species / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D017906:Siphoviridae / Q000187:drug effects* / Q000235:genetics / Q000528:radiation effects / Q000648:ultrastructure; D013481:Superoxides / Q000378:metabolism; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology*; D014466:Ultraviolet Rays
D000998:Antiviral Agents; D004279:DNA, Viral; D010083:Oxidants, Photochemical; D017382:Reactive Oxygen Species; D013481:Superoxides; C009495:titanium dioxide; D012710:Serum Albumin, Bovine; D017665:Hydroxyl Radical; D014025:Titanium