Crystallization and preliminary crystallographic studies of a phospholipase A2 from the venom of the Brazilian snake Bothrops moojeni.
A phospholipase A(2) purified from the venom of the snake Bothrops moojeni has been crystallized by vapour-diffusion techniques in hanging drops at 291 K. The crystals, which were grown in the absence of Ca(2+), belong to the cubic system, space group P432, with unit-cell parameters a = b = c = 91.86 A, and contain one molecule in the asymmetric unit (V(M) = 2.71 A(3) Da(-1)). X-ray diffraction experiments provide data to 2.35 A resolution collected on a rotating-anode home source at cryogenic temperatures. The structure has been solved via molecular-replacement techniques using a single monomer of the crystallographic structure of the phospholipase from the Western diamondback rattlesnake (Crotalus atrox) as a search model.
Instituto de Física de São Carlos, Universidade de São Paulo, CP 369, 13560-970 São Carlos SP, Brazil. mcn22@cornell.edu
M C
MC
Nonato
R C
RC
Garratt
Y P
YP
Mascarenhas
W D
WD
Jesus
M T
MT
Assakura
S M
SM
Serrano
G
G
Oliva
D000818:Animals; D017837:Bothrops; D003435:Crotalid Venoms / Q000201:enzymology*; D017839:Crotalus; D003460:Crystallization; D010741:Phospholipases A / Q000737:chemistry*; D054467:Phospholipases A2; D014961:X-Ray Diffraction
D003435:Crotalid Venoms; D010741:Phospholipases A; D054467:Phospholipases A2