A simple, rapid, and highly efficient gene expression system for multiheme cytochromes c.
The genes of tetraheme cytochrome c3 and hexadecaheme high-molecular-weight cytochrome c from Desulfovibrio vulgaris could be overexpressed as holoproteins in Shewanella oneidensis TSP-C using pUC-type vectors of E. coli. Surprisingly, S. oneidensis was transformed directly by pUC-type vectors through electroporation. The yields of the recombinant proteins in this expression system were much higher than the previously reported ones.
Ozawa
K
K
Department of Chemistry and Biotechnology, Faculty of Engineering, Yokohama National University, Japan.
Yasukawa
F
F
Fujiwara
Y
Y
Akutsu
H
H
D003574:Cytochrome c Group / Q000235:genetics* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D009682:Magnetic Resonance Spectroscopy; D010084:Oxidation-Reduction; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D020592:Shewanella / Q000235:genetics* / Q000378:metabolism
D003574:Cytochrome c Group; D011994:Recombinant Proteins