Detection of cell membrane proteins that interact with virulent infectious bursal disease virus.
To detect the molecules that interact with infectious bursal disease virus (IBDV), the chicken B lymphoblastoid cell line, LSCC-BK3, which is permissive for virulent IBDV infection was investigated. The sodium dodecyl sulfate-solubilized plasma membrane fraction from the cells was subjected to a virus overlay protein binding assay. The IBDV specifically bound to proteins in LSCC-BK3 plasma membranes with molecular weights of 70, 82 and 110 kDa. This is the first report to demonstrate cellular molecules that interact with virulent IBDV.
Department of Veterinary Microbiology, Faculty of Agriculture, Gifu University, Japan.
A
A
Setiyono
T
T
Hayashi
T
T
Yamaguchi
H
H
Fukushi
K
K
Hirai
D000818:Animals; D018175:Birnaviridae Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D002462:Cell Membrane / Q000821:virology; D002478:Cells, Cultured; D002645:Chickens; D007243:Infectious bursal disease virus / Q000276:immunology / Q000378:metabolism / Q000472:pathogenicity*; D008565:Membrane Proteins / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D008970:Molecular Weight; D011201:Poultry Diseases / Q000276:immunology / Q000821:virology*; D011485:Protein Binding / Q000276:immunology
D008565:Membrane Proteins