Primary culture of chicken hepatocytes as an in vitro model for determining the influence of dioxin.
An easy method for primary culture of chicken hepatocytes was developed to study the influence of dioxin on birds. Chicken hepatocytes could maintain gene expression and protein secretion of albumin for a long period in serum-free medium with free atmosphere exchange at 37 degrees C. Moreover, the cells showed a sensitive response to 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD) by monitoring the expression of P450 1A, theta GST (theta-GST) and albumin genes.
Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University, Japan. hou@chem.agri.kagoshima-u.ac.jp
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Kunitake
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Kusuda
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Fujii
D000418:Albumins / Q000235:genetics / Q000378:metabolism; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D002645:Chickens; D016895:Culture Media, Serum-Free; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D004785:Environmental Pollutants / Q000494:pharmacology*; D005786:Gene Expression Regulation; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D008969:Molecular Sequence Data; D000072317:Polychlorinated Dibenzodioxins / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism
D000418:Albumins; D016895:Culture Media, Serum-Free; D004785:Environmental Pollutants; D000072317:Polychlorinated Dibenzodioxins; D012333:RNA, Messenger; D003577:Cytochrome P-450 Enzyme System; C413545:glutathione S-transferase T1; D005982:Glutathione Transferase