Advanced technique for three-dimensional visualization of compound distributions in a rice kernel.
A three-dimensional (3D) visualization technique for the compound distribution in a rice kernel was developed. This technique is a combination of sectioning, staining, and digital image postprocessing. By using a special microtome system with adhesive tapes, a set of sequential sections of a rice kernel, which can be preserved with their own set of relative position data, was obtained. A single set of sequential sections was stained by various chemical techniques for the visualization of protein, starch, or lipid content. Each stained section was digitally captured using a CCD imaging device. As the stained areas represent areas containing dye-target complex, the distribution of each compound in the section was visualized in two dimensions. The digitally captured images of a single set of sequential sections were reconstructed to produce a 3D plotting image. As a result, the distributions of various compounds in a rice kernel could be visualized in a new 3D model.
National Food Research Institute, 2-1-2 Kannondai, Tsukuba, 305-8642 Japan. ogw@nfri.affrc.go.jp
Y
Y
Ogawa
J
J
Sugiyama
H
H
Kuensting
T
T
Ohtani
S
S
Hagiwara
X
X
Liu
M
M
Kokubo
A
A
Yamamoto
Kudoh Ki
T
T
Higuchi
D004396:Coloring Agents; D006652:Histological Techniques; D007091:Image Processing, Computer-Assisted / Q000379:methods; D012275:Oryza / Q000737:chemistry* / Q000166:cytology*; D012639:Seeds / Q000737:chemistry* / Q000166:cytology*
D004396:Coloring Agents