Effects of exogenous testosterone on isolated rabbit corpus cavernosum penis.
OBJECTIVE
To study the effects of exogenous excess of testosterone on the constricting effect of phenylephrine and endothelium-dependent and -independent relaxing effects of different agonists in the corpus cavernosum penis (CCP).


METHODS
Specimens of the CCP were obtained from rabbits testosterone for 1 and 2 months and untreated for 2 months after testosterone-treatment for 2 months. Preparations were mounted between two parallel platinum electrodes in organ baths. Responses to phenylephrine, carbachol, and sodium nitroprusside were obtained by adding the reagent cumulatively to the bath.


RESULTS
The phenylephrine-induced contractions were decreased with no change in agonist potency (pD2 value) after both 1 and 2 month testosterone-treatment and did not return to control values in corpus cavernosum obtained from rabbits untreated for 2 months after testosterone-treatment for 2 months. Testosterone treatment for 1 or 2 months increased the endothelium-dependent relaxations induced by carbachol and decreased the relaxations elicited by electric stimulation but did not affect the relaxations induced by sodium nitroprusside. These relaxant responses to carbachol and electric stimulation did not return to control values in corpus cavernosum obtained from rabbits untreated for 2 months after testosterone-treatment for 2 months. There were no significant changes in the pD2 values calculated by agonist-induced relaxation responses in all testosterone-treatment groups compared with control group.


CONCLUSIONS
The exogenous excess of testosterone plays an important role in erectile function by a direct action on the relaxant and contractile responses of CCP.
Yildirim
S
S
Department of Pharmacology, Faculty of Medicine, Cumhuriyet University, 58140 Sivas, Turkey.
Utkan
T
T
Yildirim
K
K
Sarioglu
Y
Y
D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D004558:Electric Stimulation; D012739:Gonadal Steroid Hormones / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D009126:Muscle Relaxation / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects; D010410:Penile Erection / Q000187:drug effects*; D010413:Penis / Q000502:physiology*; D011817:Rabbits; D013739:Testosterone / Q000494:pharmacology*
D012739:Gonadal Steroid Hormones; D013739:Testosterone; D002217:Carbachol