Chromosomal aberrations in lymphocytes of employees in transformer and generator production exposed to electromagnetic fields and mineral oil.
The objective was to study the risk of cytogenetic damage among high voltage laboratory workers exposed to electromagnetic fields and mineral oil. This is a cross sectional study of 24 exposed and 24 matched controls in a Norwegian transformer factory. The exposure group included employees in the high voltage laboratory and in the generator soldering department. Electric and magnetic fields and oil mist and vapor were measured. Blood samples were analyzed for chromosomal aberrations in cultured lymphocytes. In addition to conventional cultures, the lymphocytes were also treated with hydroxyurea and caffeine. This procedure inhibits DNA synthesis and repair in vitro, revealing in vivo genotoxic lesions that are repaired during conventional culturing. In conventional cultures, the exposure group and the controls showed similar values for all cytogenetic parameters. In the DNA synthesis- and repair-inhibited cultures, generator welders showed no differences compared to controls. Among high voltage laboratory testers, compared to the controls, the median number of chromatid breaks was doubled (5 vs. 2.5 per 50 cells; P<0.05) the median number of chromosome breaks was 2 vs. 0.5 (P>0.05) and the median number of aberrant cells was 5 vs. 3.5 (P<0.05). Further analysis of the inhibited culture data from this and a previous study indicated that years of exposure and smoking increase the risk of aberrations. We conclude that there was no increase in cytogenetic damage among exposed workers compared to controls in the conventional lymphocyte assay. In inhibited cultures, however, there were indications that electromagnetic fields in combination with mineral oil exposure may produce chromosomal aberrations.
Department of Occupational Medicine, National Institute of Occupational Health, Oslo, Norway. Knut.Skyberg@stami.no
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Skyberg
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Hansteen
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Vistnes
D000328:Adult; D000428:Alcohol Drinking; D002842:Chromatids / Q000187:drug effects / Q000528:radiation effects; D002869:Chromosome Aberrations; D003069:Coffee; D003430:Cross-Sectional Studies; D004260:DNA Repair / Q000187:drug effects / Q000528:radiation effects; D004261:DNA Replication / Q000187:drug effects / Q000528:radiation effects; D004574:Electromagnetic Fields; D004781:Environmental Exposure; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects*; D008875:Middle Aged; D008899:Mineral Oil / Q000633:toxicity*; D009664:Norway; D016273:Occupational Exposure; D012016:Reference Values; D012189:Retrospective Studies; D012907:Smoking; D011795:Surveys and Questionnaires
D003069:Coffee; D008899:Mineral Oil