Identification of methyl halide-utilizing genes in the methyl bromide-utilizing bacterial strain IMB-1 suggests a high degree of conservation of methyl halide-specific genes in gram-negative bacteria.
Strain IMB-1, an aerobic methylotrophic member of the alpha subgroup of the Proteobacteria, can grow with methyl bromide as a sole carbon and energy source. A single cmu gene cluster was identified in IMB-1 that contained six open reading frames: cmuC, cmuA, orf146, paaE, hutI, and partial metF. CmuA from IMB-1 has high sequence homology to the methyltransferase CmuA from Methylobacterium chloromethanicum and Hyphomicrobium chloromethanicum and contains a C-terminal corrinoid-binding motif and an N-terminal methyltransferase motif. However, cmuB, identified in M. chloromethanicum and H. chloromethanicum, was not detected in IMB-1.
Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, England.
C A
CA
Woodall
K L
KL
Warner
R S
RS
Oremland
J C
JC
Murrell
I R
IR
McDonald
D020561:Alphaproteobacteria / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D003470:Culture Media; D005798:Genes, Bacterial; D006090:Gram-Negative Bacteria / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D006842:Hydrocarbons, Brominated / Q000378:metabolism*; D008780:Methyltransferases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D003470:Culture Media; D006842:Hydrocarbons, Brominated; C005218:methyl bromide; C118740:CmuA protein, Methylobacterium chloromethanicum; D008780:Methyltransferases