The use of bioluminescent biotests for study of natural and laboratory aquatic ecosystems.
A set of bioluminescent tests was developed to monitor water quality in natural and laboratory ecosystems. It consisted of four bioluminescent systems: luminous bacteria, coupled enzyme system NADH:FMN-oxidoreductase-luciferase and triplet enzyme systems with alcohol dehydrogenase and trypsin. The set of biotests was applied for a small forest pond (Siberia, Russia), laboratory microecosystems polluted with benzoquinone and a batch culture of blue-green algae. Thereby effects of natural water compared to those of models of heavy pollution and "bloom" of blue-greens on the bioluminescent tests were revealed. The set of biotests was not affected by a natural seasonal variability of water quality in the unpolluted pond, but responded to the heavy pollution and the "bloom" of blue-greens. The set of biotests could be recommended as the alarm test to control the acute toxicity of natural water bodies.
Kratasyuk
V A
VA
Krasnoyarsk State University, Russian Federation.
Esimbekova
E N
EN
Gladyshev
M I
MI
Khromichek
E B
EB
Kuznetsov
A M
AM
Ivanova
E A
EA
D016227:Benzoquinones / Q000009:adverse effects*; D001681:Biological Assay / Q000379:methods; D000458:Cyanobacteria; D017753:Ecosystem; D004784:Environmental Monitoring / Q000379:methods*; D005068:Eutrophication; D038181:FMN Reductase; D007202:Indicators and Reagents / Q000009:adverse effects*; D008163:Luminescent Measurements; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D014873:Water Pollutants / Q000032:analysis*
D016227:Benzoquinones; D007202:Indicators and Reagents; D014873:Water Pollutants; C004532:quinone; D038181:FMN Reductase; D009247:NADH, NADPH Oxidoreductases