Profiles of cytokine expression in radicular cyst-lining epithelium examined by RT-PCR.
Levels of messenger RNA (mRNA) extracted from five samples of radicular cyst-lining epithelium were analyzed for cytokines, growth factors and epithelial cell growth-related receptors by RT-PCR. All five samples expressed IL-1alpha, -1beta, IL-6, IL-8, IL-11, TGF-beta1, PDGF-A and aFGF, and receptors for EGF (c-erbB), KGF, HGF (c-met) and IL-6. Some of the specimens expressed MIP-1alpha, RANTES, GM-CSF, M-CSF, TNF-alpha, PDGF-B and bFGF, but no expression of IL-2, IL-4, IFN-gamma, IGF-I, EGF and KGF was detected. These results indicate that radicular cyst-lining epithelium, which is considered to be identical to the cell rests of Malassez, may play a role in periodontal pocket formation or apical cyst formation by interaction with surrounding connective tissue or hematopoietic cells through the expression of various cytokines.
Ohshima
M
M
Department of Biochemistry, Nihon University School of Dentistry, Tokyo, Japan.
Nishiyama
T
T
Tokunaga
K
K
Sato
S
S
Maeno
M
M
Otsuka
K
K
D016207:Cytokines / Q000096:biosynthesis*; D004847:Epithelial Cells / Q000378:metabolism*; D006133:Growth Substances / Q000096:biosynthesis*; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis; D011842:Radicular Cyst / Q000378:metabolism*; D017978:Receptors, Growth Factor / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D016207:Cytokines; D006133:Growth Substances; D012333:RNA, Messenger; D017978:Receptors, Growth Factor