Evaluation of carboxymethylcellulose, hydroxypropylmethylcellulose, and aluminum hydroxide as potential carriers for rhBMP-2.
Conventional iliac crest nonvascularized corticocancelous bone grafts and bone flaps have been used to treat bony defects. However, these treatments have some limitations, namely, the availability of donor tissue, donor site morbidity, difficulty to shape the bone flap to the defect, and complexity of the surgery. The bone morphogenetic protein (rhBMP-2) is osteoinductive. However, its implantation requires a matrix (carrier) in order to define the shape of the resulting bone and to retain the protein at the site for the time required for induction to occur. When the ideal carrier is found, an unlimited supply of material would be available for all applications where bone is needed. In this in vitro study, we evaluated the suitability of some potential carriers for rhBMP-2 by measuring the alkaline phosphatase (ALP) activity of fibroblast cultures. Either rhBMP-2 or sodium carboxymethylcellulose significantly increased the ALP activity, when used alone. When sodium carboxymethylcellulose was combined with rhBMP-2, there was an increase in the ALP activity, but lower than those obtained when the products were used alone. Hydroxypropylmethylcellulose alone did not affect ALP activity. However, the combination of rhBMP-2 with hydroxypropylmethylcellulose did not increase the ALP activity, despite the presence of rhBMP-2. Aluminium hydroxide proved to be an unsuitable rhBMP-2 adsorbent.
Plastic Surgery Department, Hospital de S. João, Alameda Prof. Hernâni Monteiro, 4202-451 Porto, Portugal. santacomba@mail.telepac.pt
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A
Santa-Comba
A
A
Pereira
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R
Lemos
D
D
Santos
J
J
Amarante
M
M
Pinto
P
P
Tavares
F
F
Bahia
D000469:Alkaline Phosphatase / Q000096:biosynthesis; D000536:Aluminum Hydroxide; D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000008:administration & dosage*; D016025:Bone Transplantation; D002266:Carboxymethylcellulose Sodium; D002478:Cells, Cultured; D004337:Drug Carriers; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D065347:Hypromellose Derivatives; D066298:In Vitro Techniques; D008422:Materials Testing; D008747:Methylcellulose / Q000031:analogs & derivatives*; D011994:Recombinant Proteins / Q000008:administration & dosage; D013524:Surgical Flaps; D016212:Transforming Growth Factor beta
C525718:BMP2 protein, human; D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D004337:Drug Carriers; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; D065347:Hypromellose Derivatives; D000536:Aluminum Hydroxide; D008747:Methylcellulose; D000469:Alkaline Phosphatase; D002266:Carboxymethylcellulose Sodium