Characterization of single-stranded DNA on chitosan-modified electrode and its application to the sequence-specific DNA detection.
Single-strand DNA could bind with chitosan on a platinum electrode via forming a tight DNA-chitosan complex. The salt concentration of the ssDNA solution had an obvious effect on the surface coverage, the immobilization was remarkably reduced at high salt concentration. The sample ssDNA immobilized on the chitosan-modified electrode can hybridize efficiently with the complementary sequences and be successfully used for the sequence-specific DNA detection. The same results could be obtained using a gold or graphite electrode modified with chitosan. The stability of this electrode has been also discussed.
Department of Chemistry, East China Normal University, Shanghai, 200062, P.R. China.
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D001704:Biopolymers / Q000737:chemistry; D002686:Chitin / Q000031:analogs & derivatives / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000032:analysis / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D004586:Electrophoresis / Q000379:methods*; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D009994:Osmolar Concentration
D001704:Biopolymers; D004277:DNA, Single-Stranded; D002686:Chitin; D006046:Gold; D006108:Graphite