Expression and function of ganglioside 9-O-acetyl GD3 in postmitotic granule cell development.
We have shown previously that the Jones monoclonal antibody (Jones mAb) recognizes 9-O-acetyl GD3 expressed during periods of neuronal migration and neurite outgrowth in the developing rat nervous system. In the present study we investigated the expression of this ganglioside in the developing cerebellum and correlated this expression with granule cell migration. Electron microscopic immunocytochemistry revealed that around the peak of cerebellar neuronal migration (7-day-old rat), 9-O-acetyl GD3 was localized at the contact sites between migrating granule cells and radial glia in the external granular layer and prospective molecular layer. In addition, using microexplant and slice cultures of the postnatal rat cerebellum, we tested whether the ganglioside detected by our antibody contribute to the regulation of neuronal migration in the cerebellar cortex. We have shown that the Jones mAb blocks the migration of neurons in a dose-dependent manner. These findings suggest strongly that 9-O-acetyl GD3 is involved in granule cell migration in the developing cerebellum.
Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-590, Brazil.
M F
MF
Santiago
M
M
Berredo-Pinho
M R
MR
Costa
M
M
Gandra
L A
LA
Cavalcante
R
R
Mendez-Otero
D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002465:Cell Movement / Q000502:physiology; D002531:Cerebellum / Q000166:cytology* / Q000254:growth & development; D005732:Gangliosides / Q000032:analysis* / Q000096:biosynthesis* / Q000276:immunology; D008938:Mitosis; D009474:Neurons / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism*; D009500:Neutralization Tests; D009924:Organ Culture Techniques; D051381:Rats; D011919:Rats, Inbred Strains
D000911:Antibodies, Monoclonal; D005732:Gangliosides; C049132:9-O-acetyl-GD3 ganglioside