Hepatocyte isolation from pig livers after warm ischaemic injury.
Hepatocyte cultures have been used extensively for a wide variety of physiological, pharmacological and experimental studies. The warm ischaemic period before isolation is kept to a minimum to achieve a high yield of cells isolated and a good viability for culture. We have recently introduced a new concept of liver resuscitation after warm ischaemia that is based on a 3-h reperfusion period with an improved perfusate and simultaneous dialysis. In this study, we applied the new technique for hepatocyte isolation from livers subjected to 80 min of complete ischaemia at 37 degrees C. Cell yield was improved by a resuscitating perfusion from 58% to 73% and viability from 39% to 76%.
Schön
M R
MR
Department of Surgery, Rudolf Virchow Klinikum, Freie Universität Berlin, Germany.
Puhl
G
G
Gerlach
J
J
Frank
J
J
Neuhaus
P
P
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001646:Bile / Q000378:metabolism; D002469:Cell Separation; D002470:Cell Survival; D002478:Cells, Cultured; D003956:Dialysis; D005260:Female; D022781:Hepatocytes / Q000166:cytology* / Q000473:pathology; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008099:Liver / Q000098:blood supply* / Q000166:cytology*; D008297:Male; D010477:Perfusion; D015424:Reperfusion; D015427:Reperfusion Injury / Q000473:pathology*; D013552:Swine; D013696:Temperature; D013997:Time Factors; D014508:Urea / Q000378:metabolism
D014508:Urea; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase