Morphological correlates of functionally defined synaptic vesicle populations.
By combining photoconversion of FM1-43-stained vesicles and electron microscopy of hippocampal synapses, we find evidence that the population of morphologically docked synaptic vesicles corresponds to the release-ready neurotransmitter quanta. Furthermore, those synaptic vesicles that are participating in cycles of exo- and endocytosis tend to be closer to the active zone than vesicles that are being held in reserve.
Howard Hughes Medical Institute and Molecular Neurobiology Lab, The Salk Institute, 10010 N. Torrey Pines Road, La Jolla, California 92037, USA.
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Stevens
D000818:Animals; D002478:Cells, Cultured; D005456:Fluorescent Dyes / Q000032:analysis; D006624:Hippocampus / Q000737:chemistry / Q000166:cytology* / Q000648:ultrastructure; D007091:Image Processing, Computer-Assisted; D009474:Neurons / Q000737:chemistry* / Q000648:ultrastructure; D018377:Neurotransmitter Agents / Q000032:analysis*; D011726:Pyridinium Compounds / Q000032:analysis; D000644:Quaternary Ammonium Compounds / Q000032:analysis; D051381:Rats; D012044:Regression Analysis; D013569:Synapses / Q000648:ultrastructure; D013572:Synaptic Vesicles / Q000737:chemistry* / Q000648:ultrastructure*
C073804:FM1 43; D005456:Fluorescent Dyes; D018377:Neurotransmitter Agents; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds