A non-surgical rat model of foreign body-associated urinary tract infection with Pseudomonas aeruginosa.
This study established a rat model of foreign body-associated urinary tract infection. A spiral polyethylene tube (PT) was placed transurethrally into the bladder without surgical manipulation, followed by transurethral inoculation with Pseudomonas aeruginosa. The persistence of P. aeruginosa in the kidneys and bladder was significantly enhanced by placement of the PT, whereas the bacteria were eliminated rapidly from the urinary tract in the animals without the PT. Scanning electron microscopy revealed a thick biofilm on the surface of the PT from the early stage of infection. Histopathologically, acute pyelonephritis was followed by chronic renal inflammation as well as continuous and sporadic polymorphonuclear leukocyte accumulation and hemorrhage in the pelvis and adjacent tissues, suggesting continuous ascending introduction of the bacteria from the biofilm adhering to the PT. We believe our model simulates the pathophysiology of foreign body-associated urinary tract infection characterized by biofilm formation on the surface of a foreign body.
New Product Research Laboratories #1, Daiichi Pharmaceutical Co., Ltd., Tokyo, Japan. kuros56b@daiichipharm.co.jp
Y
Y
Kurosaka
Y
Y
Ishida
E
E
Yamamura
H
H
Takase
T
T
Otani
H
H
Kumon
D000818:Animals; D001437:Bacteriuria / Q000209:etiology; D004195:Disease Models, Animal; D005260:Female; D005547:Foreign Bodies; D007668:Kidney / Q000382:microbiology; D011552:Pseudomonas Infections / Q000209:etiology* / Q000473:pathology; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification; D051381:Rats; D013045:Species Specificity; D001743:Urinary Bladder / Q000648:ultrastructure; D014552:Urinary Tract Infections / Q000209:etiology* / Q000473:pathology