Graded contribution of retinal maturation to the development of oxygen-induced retinopathy in rats.
OBJECTIVE
Newborn rats exposed to hyperoxia during the first days of life have been shown to exhibit not only vasculopathy but also permanent changes in the structure and function of the retina. Given that the rat retina is immature at birth and that the maturation process continues until the opening of the eyes at 14 days of life, this study was conducted to investigate the susceptibility of the retina to oxygen toxicity as a function of the degree of retinal maturity reached at the time of oxygen exposure.


METHODS
Newborn rats were exposed to hyperoxia during selected postnatal day intervals. Scotopic electroretinograms were recorded at 30 and 60 days of age, and retinal histology was obtained at the end of the study.


RESULTS
There was a strong correlation between the duration of the hyperoxic event and the structural and functional consequences in the retina. However, the repercussions were significantly more profound when the exposure to oxygen occurred within the second week of life (6-14 days), compared with earlier (0-6 days) or later periods (14-28 days).


CONCLUSIONS
The results strongly suggest that the structural and functional retinal changes secondary to postnatal hyperoxia are not only the direct consequence of exposure to high levels of oxygen (i.e., free radicals), but also are determined by the level of retinal maturity reached at the time of oxygen exposure. The results also indicate that the structural anomalies precede the functional impairments.
Dembinska
O
O
Department of Neurology and Neurosurgery, McGill University, Montreal, Quebec, Canada.
Rojas
L M
LM
Varma
D R
DR
Chemtob
S
S
Lachapelle
P
P
D000818:Animals; D000831:Animals, Newborn; D004596:Electroretinography; D006801:Humans; D018496:Hyperoxia / Q000150:complications* / Q000473:pathology; D007231:Infant, Newborn; D010100:Oxygen / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000187:drug effects / Q000254:growth & development* / Q000503:physiopathology; D012178:Retinopathy of Prematurity / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology; D013997:Time Factors
D010100:Oxygen