A bacterial artificial chromosome library of Lotus japonicus constructed in an Agrobacterium tumefaciens-transformable vector.
We constructed a BAC library of the model legume Lotus japonicus with a 6-to 7-fold genome coverage. We used vector PCLD04541, which allows direct plant transformation by BACs. The average insert size is 94 kb. Clones were stable in Escherichia coli and Agrobacterium tumefaciens.
Department of Botany, University of Queensland, Brisbane, Australia. a.men@botany.uq.edu.au
A E
AE
Men
K
K
Meksem
M A
MA
Kassem
D
D
Lohar
J
J
Stiller
D
D
Lightfoot
P M
PM
Gresshoff
D016960:Agrobacterium tumefaciens / Q000235:genetics*; D015139:Blotting, Southern; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D018745:Genome, Plant; D019684:Magnoliopsida / Q000235:genetics*; D016133:Polymerase Chain Reaction; D014170:Transformation, Genetic
D018744:DNA, Plant