Nucleotide sequence of the mouse VEGF 3'UTR and quantitative analysis of sites of polyadenylation.
Sequencing of rat and human vascular endothelial growth factor (VEGF) cDNA clones has previously identified a 3' untranslated region of approximately 1.9 kb, although the apparent site of polyadenylation differed in the two species, despite a high degree of sequence conservation in the region. Neither site is preceded by a canonical AAUAAA polyadenylation signal, a situation frequently found in genes that are subject to alternative polyadenylation. We have sequenced 2.25 kb of the 3' region of the mouse VEGF gene and mapped the usage of potential polyadenylation sites in fibroblasts cultured under both normoxic and hypoxic conditions. We find that two sites for polyadenylation are present in the mouse VEGF gene but the majority of transcripts contain the longer form of the 3'UTR and that their usage is not effected by environmental oxygen tension.
Division of Human Immunology, Hanson Centre for Cancer Research, IMVS, Frome Road, Adelaide, SA 5000, Australia.
J A
JA
Dibbens
S W
SW
Polyak
A
A
Damert
W
W
Risau
M A
MA
Vadas
G J
GJ
Goodall
D020413:3' Untranslated Regions; D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D016228:Endothelial Growth Factors / Q000235:genetics*; D006801:Humans; D008222:Lymphokines / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009711:Nucleotides; D011061:Poly A / Q000378:metabolism*; D051381:Rats; D017423:Sequence Analysis, RNA; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D020413:3' Untranslated Regions; D016228:Endothelial Growth Factors; D008222:Lymphokines; D009711:Nucleotides; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D011061:Poly A