[Isolation and characterization of polysaccharides from Tansy].
Using extraction with 0.75% aqueous ammonium oxalate, the following polysaccharide fractions were isolated: tanacetans TVF, TVS, and TVR from floscules, sprouts, and roots, respectively, of Tanacetum vulgare L., spread throughout the European North of Russia. The sugar chain of tanacetan TVF consists of D-galacturonic acid (61.4%), arabinose (14.7%), galactose (10.2%), and rhamnose (3.7%) as the main constituents as well as xylose, glucose, mannose, apiose, and 2-O-methylxylose in trace amounts. Tanacetans TVS and TVR were shown to differ in the sugar quantitative composition. They contain 67 and 28% galacturonic acid, respectively. A partial acid hydrolysis of the tanacetan TVF gave a polysaccharide fragment TVF1, alpha-1,4-D-galacturonan (GalA 98.2%). Digestion with pectinase (alpha-1,4-D-polygalacturonase) resulted in fragment TVF3, containing residues of arabinose (27.1%) and galactose (17.3%). NMR spectroscopy allowed detection of the terminal residues of alpha-Araf and beta-Galp as well as of the residues of alpha-Araf substituted in 3,5- and 5-positions. Thus, tanacetan TVF was proved to be a pectic polysaccharide.
Institute of Physiology, Komi Science Centre, Urals Branch, Russian Academy of Sciences, ul. Pervomaiskaya 50, Syktyvkar, 167982 Russia.
A Ia
AIa
Polle
R G
RG
Ovodova
A S
AS
Shashkov
Iu S
IuS
Ovodov
D019659:Asteraceae; D009682:Magnetic Resonance Spectroscopy; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*
D011134:Polysaccharides