Small volume bead assay for ovalbumin with electrochemical detection.
A bead based sandwich enzyme immunoassay coupled to electrochemical detection for ovalbumin has been developed. The enzyme label alkaline phosphatase was used to convert the substrate 4-aminophenyl phosphate to electroactive product 4-aminophenol. The detection was done in a microdrop by continuously monitoring the enzyme turnover with a rotating disk electrode. This reduces dilution of the enzyme product, a key to achieving low detection limits. The assay developed has a detection limit of 0.1 ng ml-1. Assay sensitivity in complex matrices such as food and serum was compared.
Purushothama
S
S
Department of Chemistry, University of Cincinnati, P.O. Box 210172, Cincinnati, OH 45221-0172, USA.
Kradtap
S
S
Wijayawardhana
C A
CA
Halsall
H B
HB
Heineman
W R
WR
D000818:Animals; D002417:Cattle; D004563:Electrochemistry; D005079:Excipients / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D007054:Ice Cream / Q000032:analysis*; D007124:Immunoenzyme Techniques; D008832:Microchemistry; D010047:Ovalbumin / Q000032:analysis* / Q000097:blood; D051381:Rats; D012680:Sensitivity and Specificity
D005079:Excipients; D010047:Ovalbumin