LC separation of ortho and meta isomers of celecoxib in bulk and formulations using a chiral column.
A normal phase, isocratic LC method was developed for the separation of positional isomers of celecoxib (I) using a chiral column, Chiralpak-AD. The method is useful for the quantification of ortho (II) and meta (III) forms in bulk drugs and formulation samples of celecoxib. The method has been completely validated and proven to be rugged. The limit of detection (LOD) and limit of quantitation (LOQ) of ortho and meta forms were found to be 38 ng and 116 ng respectively. The active pharmaceutical ingredient was extracted from its finished dosage form (capsule) using ethanol. The percentage recoveries of ortho isomer was found to be 99.8--102.7 and 97.8--103.2 and the percentage recoveries of meta isomer was found to be 99.3--102.6 and 99.7--104 in spiked bulk and formulation samples of celecoxib respectively.
Analytical Research, Dr.Reddy's Research Foundation, Miyapur, Hyderabad500050, India.
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D
Sreenivas Rao
M K
MK
Srinivasu
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Lakshmi Narayana
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Om Reddy
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis* / Q000302:isolation & purification; D000068579:Celecoxib; D002626:Chemistry, Pharmaceutical; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D020033:Protein Isoforms / Q000032:analysis / Q000302:isolation & purification; D011720:Pyrazoles; D011786:Quality Control; D013449:Sulfonamides / Q000032:analysis* / Q000302:isolation & purification
D000894:Anti-Inflammatory Agents, Non-Steroidal; D020033:Protein Isoforms; D011720:Pyrazoles; D013449:Sulfonamides; D000068579:Celecoxib