Vascular cell adhesion molecule-1 induced by human T-cell leukaemia virus type 1 Tax protein in T-cells stimulates proliferation of human T-lymphocytes.
Human T-cell leukaemia/lymphotropic virus type 1 (HTLV-1), aetiologically linked to lymphoproliferative as well as inflammatory diseases, infects and activates CD4(+) helper T-cells and thus alters immunoregulatory pathways. The viral regulatory Tax protein has been shown previously to induce the expression of vascular cell adhesion molecule-1 (VCAM-1) by T-cells. To determine the functional role of this adhesion molecule, Jurkat T-cells stably expressing either Tax or both Tax and Rex (another viral regulatory protein) were used in binding and coculture assays performed with either control Jurkat cells or primary human T-lymphocytes. Evidence was provided that VCAM-1 acting in synergy with leucocyte function-associated antigen-3 promotes T-cell-T-cell interactions and increases T-cell proliferation. Interestingly, Rex was found to modulate these events. These data establish that VCAM-1 induced by Tax on T-cells thus contributes to the immunopathological process triggered by HTLV-1 infection.
Immunobiologie Fondamentale et Clinique, INSERM U503, 21 Avenue Tony Garnier, 69365 Lyon Cedex 07, France2.|Virologie et Pathogénèse Virale (UMR5537), CNRS-UCB Lyon 1, Faculté de Médecine Laennec, Rue Guillaume Paradin, 69372 Lyon Cedex 08, France1.
Hélène
H
Valentin
Virologie et Pathogénèse Virale (UMR5537), CNRS-UCB Lyon 1, Faculté de Médecine Laennec, Rue Guillaume Paradin, 69372 Lyon Cedex 08, France1.
Samir
S
Hamaia
Virologie et Pathogénèse Virale (UMR5537), CNRS-UCB Lyon 1, Faculté de Médecine Laennec, Rue Guillaume Paradin, 69372 Lyon Cedex 08, France1.
Stéphane
S
König
Virologie et Pathogénèse Virale (UMR5537), CNRS-UCB Lyon 1, Faculté de Médecine Laennec, Rue Guillaume Paradin, 69372 Lyon Cedex 08, France1.
Louis
L
Gazzolo
D018968:CD58 Antigens / Q000502:physiology; D016370:Gene Products, rex / Q000502:physiology; D016356:Gene Products, tax / Q000502:physiology*; D015368:Human T-lymphotropic virus 1 / Q000502:physiology*; D006801:Humans; D019169:Jurkat Cells; D008213:Lymphocyte Activation; D013601:T-Lymphocytes / Q000276:immunology* / Q000821:virology; D019010:Vascular Cell Adhesion Molecule-1 / Q000502:physiology*
D018968:CD58 Antigens; D016370:Gene Products, rex; D016356:Gene Products, tax; D019010:Vascular Cell Adhesion Molecule-1