High level excretion of Norwalk-like virus following resolution of clinical illness.
We report the case of an elderly woman excreting high levels (about 5 x 10(5) virions per gram of faeces) of Norwalk-like virus (NLV) in the absence of any clinical symptoms of gastroenteritis. Analysis by reverse transcription, polymerase chain reaction and DNA sequencing was carried out on a 342-nucleotide region of open reading frame 1. This indicated that the NLV belonged to genogroup 2 and was more closely related to the Camberwell subgroup, the most common circulating in southeast Australia at present, than to the Norwalk and Mexico viruses.
Victorian Infectious Diseases Reference Laboratory, Carlton South, Australia. john.marshall@mh.org.au
J A
JA
Marshall
S
S
Salamone
L
L
Yuen
M G
MG
Catton
J P
JP
Wright
D000368:Aged; D017250:Caliciviridae Infections / Q000821:virology*; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis; D005243:Feces / Q000821:virology*; D005260:Female; D005759:Gastroenteritis / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D009663:Norwalk virus / Q000145:classification / Q000302:isolation & purification* / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017201:Virus Shedding
D017931:DNA Primers; D004279:DNA, Viral