Genetic diversity of Bradyrhizobium strains isolated from Arachis hypogaea.
Rhizobia are used exclusively in agricultural systems for enhancing the ability of legumes to fix atmospheric nitrogen. Knowledge about the indigenous population is necessary for the selection and application of inoculant strains. In this study, we have assessed the genetic diversity of Bradyrhizobium strains isolated from the host plant, Arachis hypogaea along the coastline of Tamil Nadu. Different populations collected from varying environmental conditions were analysed for salt and pH tolerance. Genetic diversity among the strains was studied using RAPD markers and PCR-RFLP of 16S rDNA and nifD genes. The approaches used in this study yielded consistent results, which revealed a high degree of heterogeneity among strains and detection of two distinct genetic groups.
Saleena
L M
LM
M.S. Swaminathan Research Foundation, Chennai, India.
Loganathan
P
P
Rangarajan
S
S
Nair
S
S
D010367:Arachis / Q000382:microbiology*; D020369:Bradyrhizobium / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005798:Genes, Bacterial; D014644:Genetic Variation; D009586:Nitrogen Fixation / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000032:analysis*; D019105:Random Amplified Polymorphic DNA Technique
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S