Mapping of 13 horse genes by fluorescence in-situ hybridization (FISH) and somatic cell hybrid analysis.
We report fluorescence in-situ hybridization (FISH) and somatic cell hybrid mapping data for 13 different horse genes (ANP, CD2, CLU, CRISP3, CYP17, FGG, IL1RN, IL10, MMP13, PRM1, PTGS2, TNFA and TP53). Primers for PCR amplification of intronic or untranslated regions were designed from horse-specific DNA or mRNA sequences in GenBank. Two different horse bacterial artificial chromosome (BAC) libraries were screened with PCR for clones containing these 13 Type I loci, nine of which were found in the libraries. BAC clones were used as probes in dual colour FISH to confirm their precise chromosomal origin. The remaining four genes were mapped in a somatic cell hybrid panel. All chromosomal assignments except one were in agreement with human-horse ZOO-FISH data and revealed new and more detailed information on the equine comparative map. CLU was mapped by synteny to ECA2 while human-horse ZOO-FISH data predicted that CLU would be located on ECA9. The assignment of IL1RN permitted analysis of gene order conservation between HSA2 and ECA15, which identified that an event of inversion had occurred during the evolution of these two homologous chromosomes.
Lindgren
G
G
Department of Evolutionary Biology, Norbyvägen, Uppsala University, Sweden. gabriella.lindgren@ebc.uu.se
Breen
M
M
Godard
S
S
Bowling
A
A
Murray
J
J
Scavone
M
M
Skow
L
L
Sandberg
K
K
Guérin
G
G
Binns
M
M
Ellegren
H
H
D000818:Animals; D002874:Chromosome Mapping; D015723:Gene Library; D006736:Horses / Q000235:genetics*; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D008677:Metaphase; D016133:Polymerase Chain Reaction