Determination of carvedilol in human cardiac tissue by high-performance liquid chromatography.
A new high-performance liquid chromatographic method has been developed for the determination of the beta-receptor blocker carvedilol in human cardiac tissue. After homogenizing tissue samples in a microdismembrator, carvedilol and the internal standard naftopidil are extracted with acetone. The extract is evaporated to dryness and reconstituted in a potassium acetate buffer of pH 3.5. Samples are cleaned up with solid-phase extraction columns. Carvedilol and the internal standard show recoveries of 69.8 +/- 12.2% and 63.9 +/- 9.34%, respectively. The linearity range for carvedilol is 0.01-0.35 ng/mg (parts per billion) tissue (wet weight), and the limit of quantitation is 0.01 ng/mg. The percentage coefficient of variation of the intra-assay varies between 1.45 and 5.38% and the interassay between 4.25 and 6.96%. To use as an application of the assay, the cardiac carvedilol tissue level in a patient on oral carvedilol therapy for congestive heart failure is reported.
Institut für Experimentelle und Klinische Pharmakologie und Toxikologie, Abteilung für Pharmakologie, Universitätsklinikum Hamburg-Eppendorf, Hamburg, Germany.
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D000319:Adrenergic beta-Antagonists / Q000032:analysis*; D002227:Carbazoles / Q000032:analysis*; D000077261:Carvedilol; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D009206:Myocardium / Q000737:chemistry*; D011412:Propanolamines / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results
D000319:Adrenergic beta-Antagonists; D002227:Carbazoles; D011412:Propanolamines; D000077261:Carvedilol