Using in silico transcriptomics to search for tumor-associated antigens for immunotherapy.
Immunotherapy approaches to fight cancer are based on the principle of mounting an immune response against a self-antigen expressed by the tumor cells. In order to reduce potential autoimmunity side-effects, the antigens used should be as tumor-specific as possible. A complementary approach to experimental tumor antigen discovery is to screen the human genome in silico, particularly the databases of "Expressed Sequence Tags" (ESTs), in search of tumor-specific and tumor-associated antigens. The public databases currently provide a massive amount of ESTs from several hundreds of cDNA tissue libraries, including tumoral tissues from various types. We describe a novel method of EST database screening that allows new potential tumor-associated genes to be efficiently selected. The resulting list of candidates is enriched in known genes, described as being expressed in tumor cells.
Vinals
C
C
Glaxo SmithKline Biologicals, 89 rue de l'Institut, 1330, Rixensart, Belgium. carla.vinals@sbbio.be
Gaulis
S
S
Coche
T
T
D000951:Antigens, Neoplasm / Q000235:genetics* / Q000302:isolation & purification*; D001943:Breast Neoplasms / Q000235:genetics / Q000276:immunology; D019496:Cancer Vaccines / Q000235:genetics* / Q000302:isolation & purification; D003110:Colonic Neoplasms / Q000235:genetics / Q000276:immunology; D016208:Databases, Factual; D020224:Expressed Sequence Tags; D005260:Female; D005820:Genetic Testing; D015894:Genome, Human; D006801:Humans; D007167:Immunotherapy / Q000379:methods*; D008297:Male; D009369:Neoplasms / Q000235:genetics / Q000276:immunology / Q000628:therapy*; D009375:Neoplasms, Glandular and Epithelial / Q000235:genetics / Q000276:immunology; D009857:Oncogenes; D011471:Prostatic Neoplasms / Q000235:genetics / Q000276:immunology
D000951:Antigens, Neoplasm; D019496:Cancer Vaccines