Heart allograft survival in rats following immunization with soluble peptide MHC class I donor antigens: evidence for the role of indirect recognition in rejection.
The current series of experiments addressed the question of whether indirect priming with donor MHC antigens affects heart allograft survival. LEW (RT-1(1)) rats were immunized with a mixture of two peptides corresponding to the variable region of MHC class I locus Aa antigen (alpha1 and alpha2 domain). The recipients were transplanted with a DA (RT1-1a) heart 1 month after immunization, and graft survival was closely monitored by ECG. All peptide-treated recipients presented with anti-peptide antibodies at the time of transplantation and developed a strongly accelerated graft rejection. These findings indicated that indirect recognition of MHC I donor antigens promotes heart allograft rejection.
Department of Transplantation Immunology, Institute of Immunology, Heidelberg, Germany.
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P
Terness
C
C
Dufter
S
S
Post
J
J
Thies
G
G
Otto
T
T
Koru
G
G
Opelz
D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D006084:Graft Rejection / Q000276:immunology*; D006085:Graft Survival / Q000276:immunology*; D016027:Heart Transplantation / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D007165:Immunosuppression Therapy / Q000379:methods*; D007518:Isoantibodies / Q000097:blood; D007519:Isoantigens / Q000276:immunology; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D011917:Rats, Inbred Lew; D011919:Rats, Inbred Strains; D013997:Time Factors; D014184:Transplantation, Homologous
D015395:Histocompatibility Antigens Class I; D007518:Isoantibodies; D007519:Isoantigens; D010446:Peptide Fragments