[Effects of methyl mercury chloride on nuclear factor-kappa B DNA binding activities of nuclear protein extracts from developing rat cerebra and cerebella].
The effects of methyl mercury chloride (MMC) on DNA binding activities of nuclear factor kappa B(NF-kB) in developing rat cerebella and cerebra were investigated with electrophoretic mobility shift assays(EMSAs). The bindings of NF-kB in nuclei of rat cerebra and cerebella to kB probes showed two bands on gel shift in both control and experiment groups. NF-kB I and NF-kB II DNA binding activities of nuclear protein extracts from rat cerebra exposed to MMC in uterus, was lower than control groups on postnatal day 3 and 7, while that from rat cerebella was higher than control groups. The results suggested that the reactive abilities of neural cell to MMC between cerebra and cerebella were different. In binding reaction mixture, the quantities of MMC increased with the increase of NF-kB DNA binding activities of nuclear protein extracts.
Department of Toxicology, Norman Berthun University of Medical Science, Changchun 130021, China.
L
L
Dong
Z
Z
Li
X
X
Bi
L
L
Ling
D000818:Animals; D001921:Brain / Q000254:growth & development / Q000378:metabolism*; D002531:Cerebellum / Q000254:growth & development / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005260:Female; D008767:Methylmercury Compounds / Q000633:toxicity*; D016328:NF-kappa B / Q000378:metabolism*; D009687:Nuclear Proteins / Q000302:isolation & purification / Q000378:metabolism; D011247:Pregnancy; D011485:Protein Binding / Q000187:drug effects; D051381:Rats
D008767:Methylmercury Compounds; D016328:NF-kappa B; D009687:Nuclear Proteins; D004247:DNA; C004925:methylmercuric chloride