[Study on the effects of DNA damage induced by cigarette smoke in male mice testicular cells using comet assay].
The mainstream whole smoke was collected using atmosphere collector-U shape glass plate absorption tube with poly-hole, and both dimethyl sulfoxide(DMSO) and phosphate buffer solution (PBS) were used as absorbents respectively. Comet assay was used to detect the effects of DNA damage of the two solutions of cigarette smoke in male mice testicular cells. The results showed that both the two solutions of cigarette smoke contained some DNA damage agents that could induce the DNA single strand breakage in male mice testicular cells. Besides, the solution of cigarette smoke absorbed by DMSO had stronger cytotoxicity and genotoxicity than the solution of cigarette smoke absorbed by PBS. The research also suggested that collecting cigarette smoke like this paper and detecting DNA damage effects using comet assay might be an ideal measure in studying male reproducible toxicity.
Department of Environment Health, West China University of Medical Sciences, Chengdu 610041, China.
Z
Z
Zhang
Z
Z
Heng
A
A
Li
R
R
Zhao
D000818:Animals; D002478:Cells, Cultured; D004249:DNA Damage / Q000187:drug effects*; D008297:Male; D051381:Rats; D012906:Smoke / Q000009:adverse effects*; D013737:Testis / Q000166:cytology* / Q000187:drug effects; D014026:Nicotiana / Q000009:adverse effects*
D012906:Smoke