Synthesis and LC characterization of clenbuterol molecularly imprinted polymers.
Highly selective non-covalent clenbuterol (CL) imprinted polymers were prepared using methacrylic acid as monomer and ethylene glycol dimethacrylate as cross-linking agent. HPLC experiments with columns packed with this material showed that CL are selectively recognised with respect to all other adrenergic substances studied using a phosphate buffer/acetonitrile eluent. The separation was strongly dependent on pH and the organic/aqueous phase ratio. An important contribution to the recognition mechanism from hydrophobic interactions was found at higher water content. These results demonstrate that a novel family of absorbents with high selectivity for CL was obtained which can be exploited in solid phase extractions or as recognition elements for selective sensors.
Masci
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Department of Chemistry, University of Rome La Sapienza, P.le A. Moro 5, 00185 Rome, Italy. giancarlo.masci@uniroma1.it
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D001993:Bronchodilator Agents / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D002976:Clenbuterol / Q000737:chemistry*; D003432:Cross-Linking Reagents; D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D008689:Methacrylates; D011108:Polymers; D013056:Spectrophotometry, Ultraviolet
D001993:Bronchodilator Agents; D003432:Cross-Linking Reagents; D008689:Methacrylates; D011108:Polymers; C004919:ethylene dimethacrylate; D002976:Clenbuterol