Variations in cartilage catabolism in different equine joints in response to interleukin-1 in vitro.
An explant system was used to investigate the hypothesis that cartilage from different equine joints might respond differently to challenge with interleukin-1alpha (IL-1alpha). Pairs of normal cartilage samples were taken from the metacarpophalangeal, proximal interphalangeal and distal interphalangeal joints of six horses. One of each pair was stimulated with 10 ng/ml human recombinant IL-1alpha for three days, and the supernatants and remaining cartilage explants were analysed for their total content of glycosaminoglycans. A significantly higher percentage of glycosaminoglycans was released from the cartilage of the proximal and distal interphalangeal joints than from the metacarpophalangeal joint.
Fuller
C J
CJ
Department of Clinical Veterinary Science, University of Bristol, Langford.
Barr
A R
AR
Dieppe
P A
PA
D000818:Animals; D002358:Cartilage, Articular / Q000187:drug effects / Q000378:metabolism*; D046508:Culture Techniques; D006025:Glycosaminoglycans / Q000096:biosynthesis* / Q000378:metabolism; D006736:Horses / Q000378:metabolism*; D007375:Interleukin-1 / Q000494:pharmacology / Q000502:physiology*; D007700:Kinetics; D011994:Recombinant Proteins / Q000494:pharmacology
D006025:Glycosaminoglycans; D007375:Interleukin-1; D011994:Recombinant Proteins; C020341:glucosaminoglycans