Determination of 226Ra in biological samples by adsorption on specific adsorbers.
The determination of 226Ra in biological samples, such as milk and grass, was studied. 226Ra analysis of cow's milk was studied starting from de-fatted milk. The proteins were eliminated by coagulation of the colloidal phase with trichloroacetic acid. Phosphorus was then removed by precipitating it as molybdophosphate and finally adsorption was carried out by using two different adsorbers in order to concentrate and purify radium. Lead rhodizonate (LEHRO) adsorbed on charcoal and partially reduced tin dioxide (PRTD) were utilised. A method for the determination of 226Ra in grass ashes was also investigated. The main interference, due to magnesium, hinders the use of LERHO, so the proposed procedure is based on adsorption of radium on PRTD at pH 9.5. The magnesium concentration was depleted by precipitating barium (carrier) and radium with calcium carbonate at pH 8 before the adsorption step. The high phosphorus concentration in grass also interferes in the determination of 226Ra; phosphorus was eliminated as above via molybdophosphate precipitation. The radium was carried by barium and spiked with 133Ba. The yield of the chemical procedure was evaluated on the basis of 133Ba activity. Radium samples were alpha-counted and the activity was evaluated with a suitable calibration curve. Both exchangers in the milk analysis and PRTD in grass analysis were shown to be helpful in order to set up an easily performed procedure, which allows many samples to be processed simultaneously. All the methods adopted were shown to be very sensitive. Under the experimental conditions used, with 1 L of milk or 5 g of grass ashes, the limit was about 3 mBq 226Ra L-1 milk and < 1 mBq 226Ra g-1 grass ashes.
Dipartimento de Chimica Generale, Università di Pavia, Viale Taramelli 12, 27100 Pavia, Italy.
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Crespi Caramella
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Valentini Ganzerli
D000327:Adsorption; D000818:Animals; D008892:Milk / Q000737:chemistry*; D006109:Poaceae / Q000737:chemistry*; D011848:Radioactive Pollutants / Q000032:analysis*; D011883:Radium / Q000032:analysis*; D012680:Sensitivity and Specificity
D011848:Radioactive Pollutants; D011883:Radium