Identification of hTLR10: a novel human Toll-like receptor preferentially expressed in immune cells.
Herein we describe the isolation of a cDNA encoding a novel human Toll-like receptor (hTLR) that we term hTLR10. Human TLR10 contains 811 amino acid residues. Deduced amino acid sequence analysis reveals that like the other known hTLRs (hTLR1-9) it is characterized by a signal peptide followed by multiple leucine-rich repeats (LRRs), a cysteine-rich domain, a transmembrane sequence and a cytoplasmic domain homologous to that of the human interleukin-1 receptor. Phylogenetic analysis indicates that among all the hTLRs, hTLR10 is most closely related to hTLR1 and hTLR6; the overall amino acid identity is 50% and 49%, respectively. hTLR10 mRNA is most highly expressed in lymphoid tissues such as spleen, lymph node, thymus, and tonsil. Expression analysis of cell lines indicates a predominance in a variety of immune cell types. Thus, hTLR10 is preferentially expressed in tissues and cells involved in immune responses.
Department of Immunology, The Scripps Research Institute, 10550 North Torrey Pines Road, 92037, La Jolla, CA, USA.
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D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D018076:DNA, Complementary; D015870:Gene Expression; D006801:Humans; D008198:Lymph Nodes / Q000378:metabolism; D008969:Molecular Sequence Data; D014066:Palatine Tonsil / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013154:Spleen / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism; D051201:Toll-Like Receptor 10
D018076:DNA, Complementary; D011956:Receptors, Cell Surface; C475955:TLR10 protein, human; D051201:Toll-Like Receptor 10