Age-related changes in the distribution of Kv1.1 and Kv1.2 channel subunits in the rat cerebellum.
We have revealed age-related changes in the expression patterns of Kv1.1 and Kv1.2 in the rat cerebellum for the first time. In the aged rat, immunoreactivity for Kv1.1 was increased in the cell bodies of Purkinje cells, while the staining intensity was significantly decreased in the granule cells. The cell bodies of cerebellar output neurons showed strong Kv1.1 immunoreactivity in the nucleus medialis, interpositus and lateralis of the aged rat. Kv1.2 immunoreactivity was found in some interneurons with their processes in this region of the aged rat. Image analysis demonstrated that immunoreactivities for Kv1.1 and Kv1.2 were increased specifically in the cell bodies of cerebellar output neurons of the aged rat. This study may provide useful data for future investigations on the channels that cause brain diseases and age-related disorders.
Department of Anatomy, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-Gu, 110-799, Seoul, South Korea.
Y H
YH
Chung
C M
CM
Shin
M J
MJ
Kim
B K
BK
Lee
C I
CI
Cha
D000375:Aging / Q000378:metabolism*; D000818:Animals; D002531:Cerebellum / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D007395:Interneurons / Q000737:chemistry / Q000378:metabolism; D051662:Kv1.1 Potassium Channel; D051663:Kv1.2 Potassium Channel; D015221:Potassium Channels / Q000032:analysis / Q000378:metabolism*; D024642:Potassium Channels, Voltage-Gated; D051381:Rats; D017207:Rats, Sprague-Dawley
C496972:Kcna2 protein, rat; D051663:Kv1.2 Potassium Channel; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D051662:Kv1.1 Potassium Channel