glnD and mviN are genes of an essential operon in Sinorhizobium meliloti.
To evaluate the role of uridylyl-transferase, the Sinorhizobium meliloti glnD gene was isolated by heterologous complementation in Azotobacter vinelandii. The glnD gene is cotranscribed with a gene homologous to Salmonella mviN. glnD1::Omega or mviN1::Omega mutants could not be isolated by a powerful sucrose counterselection procedure unless a complementing cosmid was provided, indicating that glnD and mviN are members of an indispensable operon in S. meliloti.
Laboratoire de Biologie Moléculaire des Relations Plantes-Microorganismes, INRA/CNRS, 31326 Castanet-Tolosan Cedex, France.
P A
PA
Rudnick
T
T
Arcondéguy
C K
CK
Kennedy
D
D
Kahn
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D005798:Genes, Bacterial; D020043:Genes, Essential; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009713:Nucleotidyltransferases / Q000235:genetics* / Q000378:metabolism; D009876:Operon; D051922:PII Nitrogen Regulatory Proteins; D010957:Plasmids; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D016962:Sinorhizobium meliloti / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D051922:PII Nitrogen Regulatory Proteins; D009713:Nucleotidyltransferases; C022515:regulatory protein uridylyltransferase