Comparative genomic hybridization (CGH) analysis of stage 4 neuroblastoma reveals high frequency of 11q deletion in tumors lacking MYCN amplification.
We have studied the occurrence and association of 11q deletions with other chromosomal imbalances in Stage 4 neuroblastomas. To this purpose we have performed comparative genomic hybridization (CGH) analysis on 50 Stage 4 neuroblastomas and these data were analyzed together with those from 33 previously published cases. We observed a high incidence of 11q deletion in Stage 4 neuroblastoma without MYCN amplification (59%) whereas 11q loss was only observed in 15% of neuroblastomas with MYCN-amplification (p = 0.0002) or 11% of cases with 1p deletion detected by CGH (p = 0.0001). In addition, 11q loss showed significant positive correlation with 3p loss (p = 0.0002). Event-free survival was poor and not significantly different for patients with or without 11q deletion. Our study provides further evidence that Stage 4 neuroblastomas with 11q deletions represent a distinct genetic subgroup that typically shows no MYCN-amplification nor 1p deletion. Moreover, it shows that neuroblastomas with 11q deletion also often present 3p deletion. This genetic subgroup shows a similar poor prognosis as MYCN amplified 4 neuroblastomas.
Plantaz
D
D
Department of Paediatrics, and Laboratory of Cancer Biology. University Hospital Centre of Grenoble, Grenoble, France. dominique.plantaz@ujf-grenoble.fr
Vandesompele
J
J
Van Roy
N
N
Lastowska
M
M
Bown
N
N
Combaret
V
V
Favrot
M C
MC
Delattre
O
O
Michon
J
J
Bénard
J
J
Hartmann
O
O
Nicholson
J C
JC
Ross
F M
FM
Brinkschmidt
C
C
Laureys
G
G
Caron
H
H
Matthay
K K
KK
Feuerstein
B G
BG
Speleman
F
F
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D002869:Chromosome Aberrations; D002872:Chromosome Deletion; D002878:Chromosomes, Human, Pair 1; D002880:Chromosomes, Human, Pair 11; D002893:Chromosomes, Human, Pair 3; D018572:Disease-Free Survival; D005260:Female; D015894:Genome, Human; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007223:Infant; D008297:Male; D008957:Models, Genetic; D015337:Multicenter Studies as Topic; D009154:Mutation; D009362:Neoplasm Metastasis; D009447:Neuroblastoma / Q000175:diagnosis / Q000235:genetics* / Q000401:mortality; D009693:Nucleic Acid Hybridization; D011379:Prognosis; D013997:Time Factors; D014407:Tumor Cells, Cultured