Lidocaine and surgical modification reduces mortality in a rat model of cardiac failure induced by coronary artery ligation.
Coronary artery ligation in the rat provides a useful experimental model of cardiac failure; however, this procedure carries with it a high mortality rate (50%). In this study, we used lidocaine (10 mg/kg, i.m.) before coronary artery ligation and 2 h after surgery to minimise the incidence of ventricular fibrillation (VF) that leads to sudden death in this model. We found that coronary artery ligation, using lidocaine in conjunction with a modified surgical procedure, had a mortality rate of 15%, much lower than reported in previous studies using this model. These modifications allow for the production of larger infarcts with 29% of animals having an infarct size > 50% of the epicardial surface. Infarct size in our myocardial infarction (MI) group varied between 5% and 75% of the left ventricular (LV) surface area resulting in a mean infarct size of 41.3 +/- 1.3% for the epicardial surface and 40.0 +/- 1.3% for the endocardial surface.
Department of Pharmacology, Monash University, Victoria 3800, Australia. andrew.kompa@med.monash.edu.au
A R
AR
Kompa
R J
RJ
Summers
D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000627:therapeutic use*; D001835:Body Weight; D003331:Coronary Vessels / Q000502:physiology*; D006333:Heart Failure / Q000188:drug therapy / Q000601:surgery / Q000628:therapy*; D008012:Lidocaine / Q000627:therapeutic use*; D008026:Ligation; D008297:Male; D009203:Myocardial Infarction / Q000473:pathology; D009206:Myocardium / Q000473:pathology; D009929:Organ Size; D051381:Rats; D017207:Rats, Sprague-Dawley
D000889:Anti-Arrhythmia Agents; D008012:Lidocaine