Selective differentiation of mammalian bone marrow stromal cells cultured on three-dimensional polymer foams.
Bone marrow stromal cells (BMSC) are pluripotent progenitor cells that can regenerate different skeletal tissues in response to environmental signals. In this study, we used highly porous, structurally stable three-dimensional polymer foams in conjunction with specific regulatory molecules to selectively differentiate mammalian BMSC into either cartilaginous or bone-like tissues. Bovine BMSC were expanded in monolayers and cultured on 5-mm-diameter, 2-mm-thick foams made of poly(lactic-co-glycolic acid) and poly(ethylene glycol). Constructs maintained their original size and shape for up to 4 weeks of culture and supported BMSC growth and production of extracellular matrix (ECM). By proper use of chondrogenic (dexamethasone, insulin, transforming growth factor-beta1) or osteogenic (dexamethasone, beta-glycerophosphate) medium supplements, we could control whether the generated ECM was cartilaginous (containing collagen type II and sulfated glycosaminoglycans) or bone-like (containing osteocalcin, osteonectin, and mineralized foci). After 4 weeks of cultivation, cartilaginous and bone-like ECM were uniformly distributed throughout the construct volume and respectively represented 34.2 +/- 9.3% and 12.6 +/- 3.2% of the total available area. BMSC culture on poly(lactic-co-glycolic acid)/poly(ethylene glycol) foams provides a three-dimensional model system to study the development of mesenchymal tissues in vitro and has potential applications in engineering autologous grafts for skeletal tissue repair.
Martin
I
I
Division of Health Sciences & Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Shastri
V P
VP
Padera
R F
RF
Yang
J
J
Mackay
A J
AJ
Langer
R
R
Vunjak-Novakovic
G
G
Freed
L E
LE
D000818:Animals; D001672:Biocompatible Materials; D001698:Biomedical Engineering; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects; D002356:Cartilage / Q000254:growth & development; D002417:Cattle; D002454:Cell Differentiation; D002478:Cells, Cultured; D046508:Culture Techniques; D003907:Dexamethasone / Q000494:pharmacology; D005994:Glycerophosphates / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis; D011092:Polyethylene Glycols; D011098:Polyglactin 910; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D001672:Biocompatible Materials; D005994:Glycerophosphates; D007328:Insulin; D016212:Transforming Growth Factor beta; C102418:poly(lactic-glycolic acid)-poly(ethyleneglycol) copolymer; D011098:Polyglactin 910; D011092:Polyethylene Glycols; D003907:Dexamethasone; C031463:beta-glycerophosphoric acid