Fibrin stimulates microfilament reorganization and IL-1beta production in human monocytic THP-1 cells.
Fibrin plays important roles in the wound healing processes, including blood clotting and platelet aggregation. Additional activities of fibrin were found in this study, which utilizes human THP-1 cells treated 1,25-(OH)2 vitamin D3 and plasminolytic fragments derived from fibrin. Coated fibrin fragment E on culture plates induced cell adhesions and morphological changes of the THP-1 cells, being resembled to tissue macrophages. Morphological changes of the THP-1 cells were caused by microfilament reorganization. IL-1beta production was increased in the THP-1 cells by adherent fibrin fragment E, but not by fibrin fragment D or by fibrinogen fragment E. The elevation of IL-1beta production is caused by transcriptional activation. Incubation with cytochalacin D, an actin polymerization inhibitor, prevents both microfilament reorganization and morphological changes, but has no effect on the IL-1beta production stimulated by fibrin fragment E. This data suggests that the IL-1beta production in the THP-1 cells do not require microfilament reorganization and integrin aggregation. Taken together, these results indicate that fibrin matrix plays an additional role in the stimulation of monocytes for production of IL-1beta, morphological changes and cell adhesion, resulting in the facilitation of the wound healing processes.
Division of Science Education, Kangwon National University, Choonchun, Korea.
M E
ME
Lee
S M
SM
Kweon
K S
KS
Ha
S U
SU
Nham
D008841:Actin Cytoskeleton / Q000187:drug effects / Q000378:metabolism*; D000933:Antifibrinolytic Agents / Q000494:pharmacology*; D002117:Calcitriol / Q000494:pharmacology; D002120:Calcium Channel Agonists / Q000494:pharmacology; D002448:Cell Adhesion / Q000276:immunology; D002478:Cells, Cultured; D005338:Fibrin Fibrinogen Degradation Products / Q000494:pharmacology*; D015870:Gene Expression / Q000276:immunology; D006801:Humans; D016167:Integrin alphaXbeta2 / Q000235:genetics; D007375:Interleukin-1 / Q000096:biosynthesis*; D016177:Macrophage-1 Antigen / Q000235:genetics; D009000:Monocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis
D000933:Antifibrinolytic Agents; D002120:Calcium Channel Agonists; D005338:Fibrin Fibrinogen Degradation Products; D016167:Integrin alphaXbeta2; D007375:Interleukin-1; D016177:Macrophage-1 Antigen; D012333:RNA, Messenger; C036309:fibrin fragment D; D002117:Calcitriol