Fluconazole disk diffusion test with methylene blue- and glucose-enriched Mueller-Hinton agar for determining susceptibility of Candida species.
A 25-microg fluconazole disk diffusion test using a Mueller-Hinton agar plate containing 2% glucose and 5 microg of methylene blue/ml (GM-MH) was compared to the macrodilution reference method for 210 Candida species. The GM-MH agar plate was read at 24 h. The predictive values of disks with susceptible, intermediate, and resistant results on the GM-MH agar plate at 24 h were 97.1, 56.3, and 76.5%, respectively.
Lee
S C
SC
Division of Infectious Diseases, Chang Gung Memorial Hospital, Keelung, Taipei, Taiwan, Republic of China. Ruby800@adm.cgmh.com.tw
Fung
C P
CP
Lee
N
N
See
L C
LC
Huang
J S
JS
Tsai
C J
CJ
Chen
K S
KS
Shieh
W B
WB
D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects*; D003470:Culture Media; D015725:Fluconazole / Q000494:pharmacology*; D005947:Glucose; D006801:Humans; D008751:Methylene Blue; D008826:Microbial Sensitivity Tests / Q000379:methods
D000935:Antifungal Agents; D003470:Culture Media; D015725:Fluconazole; D005947:Glucose; D008751:Methylene Blue