[Association of glucokinase gene with gestational diabetes mellitus in Chinese].
OBJECTIVE
To evaluate the role of glucokinase(GCK) gene in the pathogenesis of gestational diabetes mellitus (GDM) in Chinese.


METHODS
Two microsatellite polymorphisms, GCK1 and GCK2 which located at approximately 10 Kb 3' and 6 Kb 5', respectively, of the human glucokinase gene on chromosome 7p13, were genotyped in 40 unrelated gestational diabetics and 43 controls.


RESULTS
Four alleles (A, B, C, D) and seven genotypes were identified at the GCK1 locus. There was no significant difference in allele and genotype frequency between GDM and control groups at the GCK1 locus. For GCK2, four alleles(1,2,3,4) and eight genotypes were detected. When compared with control subjects, the GDM group had a much less frequency of the allele 2 (51.3% vs 69.8%, chi 2 = 5.965, P = 0.015), and a much more frequency of allele 3(31.3% vs 17.4%, chi 2 = 4.321, P = 0.038). Nine haplotypes of GCK1 and GCK2 were observed and haplotype B/2 was much less in GDM group(1.9% vs 19.4%, RR = 0.078 6, P = 0.003). Among the GDM patients, comparing with carriers of other alleles, the carriers of allele 3 of GCK2 locus had significantly elevated fasting and 2 hour's blood glucose levels during OGTT, and their insulin levels at 2nd and 3rd hour during the OGTT test were significantly decreased. Our results suggest that GCK gene was associated with Chinese GDM, and haplotype of GCK1/GCK2 B/2 was a protective factor for GDM.
Department of Obstetrics, People's Hospital, Beijing Medical University, Beijing 100044.
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H
Han
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S
Wang
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L
Ji
D000328:Adult; D000483:Alleles; D016640:Diabetes, Gestational / Q000209:etiology / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005941:Glucokinase / Q000235:genetics*; D006801:Humans; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D011247:Pregnancy
D005941:Glucokinase