Synthesis and characterization of fluorescent cobalamin (CobalaFluor) derivatives for imaging.
Fluorescent derivatives of cobalamin have been prepared by linking fluorophores to cobalamin through a propylamide spacer. Fluorescein, naphthofluorescein, and Oregon Green derivatives have been prepared in good yield by reaction of the fluorophore NHS-ester with beta-(3-aminopropyl)cobalamin to form fluorescent cobalamin conjugates (CobalaFluors) that are potentially suitable for the in vitro and in vivo imaging of transcobalamin receptors on cancer cells.
Smeltzer
C C
CC
Department of Chemistry, University of Utah, 315 S. 1400 E., Salt Lake City, Utah 84112-0850, USA.
Cannon
M J
MJ
Pinson
P R
PR
Munger
J D
JD
West
F G
FG
Grissom
C B
CB
D001896:Boron Compounds; D005452:Fluoresceins / Q000138:chemical synthesis* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D007202:Indicators and Reagents; D008968:Molecular Conformation; D015394:Molecular Structure; D009369:Neoplasms / Q000175:diagnosis; D011956:Receptors, Cell Surface / Q000032:analysis; D013053:Spectrophotometry; D014805:Vitamin B 12 / Q000138:chemical synthesis* / Q000737:chemistry
D001896:Boron Compounds; D005452:Fluoresceins; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D011956:Receptors, Cell Surface; C426407:n-fluoroacyl oxazaborolidine; C000601316:naphthofluorescein; C028176:transcobalamin receptor; D014805:Vitamin B 12