In vivo microdialysis in the visual cortex of awake cat. III: histological verification.
In vivo microdialysis sampling extracellular excitatory and inhibitory amino acids from the striate cortex of mammals will provide important information for visual system research. To facilitate the interpretation of microdialysis results, this protocol critically examines: (1) the location of probe implantation in the visual cortex using Nissl staining; (2) the morphological changes after probe implantation by visualization of neurons containing glutamate; (3) the morphological changes after probe implantation by visualization of gliosis using glial fibrillary acidic protein (GFAP) immunocytochemistry; (4) the implantation of the probe in sensory-deprived versus non-deprived cortical regions by visualization of neurons containing c-Fos protein after limited retinal lesion. The histochemical and immunocytochemical methods of Glu, GFAP and c-Fos used are described.
Laboratory of Neuroendocrinology and Immunological Biotechnology, Zoological Institute, Katholieke Universiteit Leuven, B-3000, Leuven, Belgium. quying@mail.nih.gov
Y
Y
Qu
E
E
Van der Gucht
A
A
Massie
E
E
Vandenbussche
F
F
Vandesande
L
L
Arckens
D000818:Animals; D048588:Benzoxazines; D002415:Cats; D004396:Coloring Agents; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D017551:Microdialysis / Q000379:methods*; D010078:Oxazines; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D012683:Sensory Deprivation / Q000502:physiology; D013194:Staining and Labeling; D014785:Vision, Ocular / Q000502:physiology; D014793:Visual Cortex / Q000378:metabolism* / Q000473:pathology
D048588:Benzoxazines; D004396:Coloring Agents; D005904:Glial Fibrillary Acidic Protein; D010078:Oxazines; D016760:Proto-Oncogene Proteins c-fos; C028911:cresyl violet; D018698:Glutamic Acid