Deoxynucleoside anabolic enzyme levels in acute myelocytic leukemia and chronic lymphocytic leukemia cells.
The deoxynucleoside kinase reaction is often rate-limiting in the anabolism of pharmacologically active anti-cancer nucleosides. The levels of thymidine kinase (TK), deoxycytidine kinase, deoxyguanosine kinase (dGK), and thymidylate kinase were determined in leukocyte extracts from patients with chronic lymphocytic leukemia (CLL) and acute myelocytic leukemia (AML). The extracts from AML patients showed significantly higher TK activity than the ones from CLL patients. There were no differences in the levels of the other three kinases. In the case of dGK, the determinations were carried out with both an immunoblotting assay and selective enzyme activity measurements.
Department of Infectious Diseases, Huddinge Hospital, SE-141 86, Huddinge, Sweden.
B
B
Jacobsson
F
F
Albertioni
S
S
Eriksson
D015153:Blotting, Western; D003842:Deoxycytidine Kinase / Q000096:biosynthesis; D003853:Deoxyribonucleosides / Q000378:metabolism; D006801:Humans; D007945:Leukemia, Lymphoid / Q000201:enzymology*; D015470:Leukemia, Myeloid, Acute / Q000201:enzymology*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D009703:Nucleoside-Phosphate Kinase / Q000096:biosynthesis*; D010770:Phosphotransferases / Q000096:biosynthesis*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000096:biosynthesis; D013937:Thymidine Kinase / Q000096:biosynthesis
D003853:Deoxyribonucleosides; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C019165:deoxyguanosine kinase; D013937:Thymidine Kinase; D003842:Deoxycytidine Kinase; D009703:Nucleoside-Phosphate Kinase; C020093:dTMP kinase