TBP-interacting protein TIP120A is a new global transcription activator with bipartite functional domains.
BACKGROUND
We previously identified a TBP (TATA-binding protein)-interacting protein 120A (TIP120A) from rat liver nuclear extracts. TIP120A is thought to be a unique global transcription factor that can interact with TBP and can stimulate all classes of eukaryotic transcription.


RESULTS
We produced various truncation proteins of TIP120A to delineate its functional domains. TIP120A binds to TBP in the acidic amino acid-rich N-terminal region and in the leucine-rich C-terminal region. These regions exhibited an ability to stimulate basal transcription in vitro. In addition, these two regions overlap with domains that facilitate nonspecific DNA-binding of RNA polymerase II. The sequences of these two regions are significantly conserved among TIP120A homologues of eukaryotes.


CONCLUSIONS
TIP120A is a bipartite transcription factor, and both N-terminal and C-terminal regions exhibit TBP-binding activity and stimulate the basal transcription ability.
Department of Biology, Faculty of Science, Chiba University, Japan.
K
K
Kayukawa
Y
Y
Kitajima
T
T
Tamura
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D012319:RNA Polymerase II / Q000378:metabolism; D051381:Rats; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid; D035181:TATA-Box Binding Protein; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
C486317:CAND1 protein, human; C102896:Cand1 protein, rat; D004268:DNA-Binding Proteins; D035181:TATA-Box Binding Protein; D015534:Trans-Activators; D014157:Transcription Factors; D012319:RNA Polymerase II