Imaging of the lectin-labeled cell surface of human lymphocytes by the use of atomic force microscope.
The atomic force microscope (AFM) is a new useful tool to examine the surface structure of specimens with a higher resolution than the conventional scanning electron microscope. In the present study, we used the AFM to observe the surface of paraformaldehyde-fixed human lymphocytes processed for histochemistry using a biotinylated lectin, wheat germ agglutinin, followed by colloidal gold and silver-enhancement method. Before the treatment, no particles were attached to the cell surface. After treatment, many particles about 100 to 150 nm in diameter were visualized on it. Since we could observe the same cells on the slide glass before and after treatment, the AFM has the advantage to enable us the repeated imaging of samples treated with various kinds of histochemistries.
Department of Veterinary Anatomy, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
M
M
Sakaue
K
K
Taniguchi
D019921:Biotinylation; D002462:Cell Membrane / Q000737:chemistry; D006651:Histocytochemistry; D006801:Humans; D008214:Lymphocytes / Q000737:chemistry / Q000648:ultrastructure*; D018625:Microscopy, Atomic Force; D016622:Silver Staining; D013499:Surface Properties; D014909:Wheat Germ Agglutinins / Q000737:chemistry
D014909:Wheat Germ Agglutinins