Effect of mycobacterial phospholipids on interaction of Mycobacterium tuberculosis with macrophages.
This study demonstrates that pretreatment of macrophages with phosphatidylinositol, of either soya bean or mycobacterial origin, results in a down-regulation of the binding and uptake of Mycobacterium tuberculosis by the phagocytes. We also describe the novel observation that cardiolipin induces an increase in the binding and uptake of M. tuberculosis by macrophages. Neither phospholipid interacts with macrophages via the 2F8 epitope of scavenger receptor A, and treatment of macrophages with either phospholipid results in a down-regulation of CR3 function and tumor necrosis factor alpha production by the phagocyte. We have also shown that the ability of macrophages to interact with mycobacteria is greatly affected by an as yet unidentified product from the interaction of chloroform and polypropylene tubes.
Division of Infectious and Immunological Diseases, British Columbia's Children's Hospital, University of British Columbia, Vancouver, British Columbia, Canada.
L M
LM
Thorson
D
D
Doxsee
M G
MG
Scott
P
P
Wheeler
R W
RW
Stokes
D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects*; D002308:Cardiolipins / Q000494:pharmacology*; D005260:Female; D008264:Macrophages / Q000382:microbiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009169:Mycobacterium tuberculosis / Q000502:physiology*; D010716:Phosphatidylinositols / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D002308:Cardiolipins; D010716:Phosphatidylinositols; D014409:Tumor Necrosis Factor-alpha