[Influence of eosin y and orthovanadate on the steady-state of Ca(+)2 entry into secretory cells of exocrine glands and their spontaneous secretion].
Ca(2+)-pump blocators in low concentrations (eozyn Y up to 5 mM and ortovanadate up to 40 mM) essentially increases of Ca2+ content in salivary gland of Chironomus plumosus larvae's and spontaneous protein secretion. It was shown that eozyn Y much more effectively suppresses of plasma membrane Ca(2+)-pump then ortovanadate. Eozyn Y and ortovanadate in higher concentrations essentially decrease of Ca2+ content in glands and spontaneous protein secretion. The former is evoked by suppression of endoplasm reticulum Ca(2+)-pump, decreasing of Ca2+ influx in cells following by diminishing of Ca2+ transmembrane gradient. Therefore, energydependent Ca2+ transporting systems of plasma membrane and endoplasm reticulum effectively regulate steady-state Ca2+ entry in secretory cells of Chironomus plumosus salivary glands and maintain relatively low level of spontaneous secretion.
Fedirko
N V
NV
Ivan Franko National University, Lviv, Ukraine.
Klevets'
M Iu
MIu
D000818:Animals; D002118:Calcium / Q000032:analysis; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002683:Chironomidae; D004801:Eosine Yellowish-(YS) / Q000494:pharmacology*; D007814:Larva; D012469:Salivary Glands / Q000187:drug effects* / Q000378:metabolism; D014638:Vanadates / Q000494:pharmacology*
D002121:Calcium Channel Blockers; D014638:Vanadates; D002118:Calcium; D004801:Eosine Yellowish-(YS)