Acute changes in plasma levels of hepatocyte growth factor during low-density lipoprotein apheresis.
Hepatocyte growth factor (HGF) is a mesenchyme-derived pleiotropic factor, and angiogenesis is included in a variety of its functional effects. HGF levels were measured in 5 sessions of low-density lipoprotein (LDL) apheresis in 3 patients with severe hypercholesterolemia. Blood was collected at the start (T0) and at 1,000 ml (T1), 2,000 ml (T2), and 3,000 ml (T3) plasma treatments. During LDL apheresis, HGF levels increased from 1.59 +/- 0.78 (mean +/- SE, n = 5) ng/ml at T0 to 6.64 +/- 0.97 at T1, 6.28 +/- 0.97 at T2, and 5.20 +/- 0.94 at T3. In one apheresis session, HGF increased immediately at the 100 ml plasma treatment stage. HGF was adsorbed completely by a dextran-sulfate (DS) column. Despite the adsorption by the DS column, HGF in the patient blood increased to the levels with functional effects. The improvement of ischemic symptoms due to LDL apheresis may be related to the angiogenic activities of HGF.
Department of Clinical Research, Tohsei National Hospital, Sunto-gun, Shizuoka, Japan.
S
S
Kojima
M
M
Shida
K
K
Tanaka
H
H
Takano
H
H
Yokoyama
M
M
Kuramochi
D000704:Analysis of Variance; D001781:Blood Component Removal / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D017228:Hepatocyte Growth Factor / Q000097:blood*; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood / Q000628:therapy*; D008077:Lipoproteins, LDL / Q000302:isolation & purification*; D008297:Male; D008875:Middle Aged
D008077:Lipoproteins, LDL; D017228:Hepatocyte Growth Factor