Effect of ultrasonic treatment on the molecular weight of carboxymethylated chitin-glucan complex from Aspergillus niger.
Different factors affecting the efficiency of the ultrasonic depolymerization of the high-molecular-weight carboxymethylated chitin-glucan prepared from the fungal mycelium of Aspergillus niger have been investigated. The influence of the following parameters was examined: concentration of the chitin-glucan complex, duration of ultrasonic irradiation, reaction temperature and volume of the ultrasonicated solution. The optimized conditions for the efficient ultrasonic depolymerization include: polysaccharide concentration--0.2 mg ml-1; volume of the sonicated solution--25 ml; duration of the sonication--10 min; and constant cooling of the sonicated sample in an ice-water bath.
Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 842 38 Bratislava, Slovak Republic.
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Machová
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Kvapilová
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Kogan
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J
Sandula
D001234:Aspergillus niger / Q000737:chemistry*; D002686:Chitin / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005936:Glucans / Q000737:chemistry*; D008970:Molecular Weight; D014465:Ultrasonics
D005936:Glucans; D002686:Chitin