Increased expression of Fas (APO-1, CD95) on CD4+ and CD8+ T lymphocytes during total body irradiation.
Fas/APO-1 (CD95) is a cell surface molecule that can transduce apoptotic signals into cells. We examined the expression of Fas antigen on CD4+ and CD8+ T cells of patients who received total body irradiation (TBI) as a preparative regimen for allogeneic bone marrow transplantation. Numbers of peripheral blood lymphocytes were significantly reduced after TBI. Cytofluorometric analysis revealed a significantly higher expression of Fas on CD4+ and CD8+ T cells after TBI. Serum soluble Fas concentrations were significantly elevated after TBI. Changes in the Fas system were therefore accompanied by TBI-induced lymphocytopenia, suggesting that Fas plays a role in irradiation-induced apoptosis in vivo.
Second Department of Internal Medicine, Hyogo College of Medicine, 1-1 Mukogawa-cho, Nishinomiya, Hyogo 663-8501, Japan. fuji-y@hyo-med.ac.jp
Y
Y
Fujimori
K
K
Saheki
H
H
Itoi
T
T
Okamamoto
E
E
Kakishita
D000328:Adult; D017209:Apoptosis / Q000528:radiation effects; D016026:Bone Marrow Transplantation; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism / Q000528:radiation effects; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism / Q000528:radiation effects; D005260:Female; D005434:Flow Cytometry; D019337:Hematologic Neoplasms / Q000150:complications / Q000628:therapy; D006801:Humans; D018655:Lymphocyte Count; D008231:Lymphopenia / Q000209:etiology; D008297:Male; D013601:T-Lymphocytes / Q000378:metabolism*; D014184:Transplantation, Homologous; D015854:Up-Regulation; D014916:Whole-Body Irradiation; D019014:fas Receptor / Q000097:blood / Q000378:metabolism* / Q000528:radiation effects
D019014:fas Receptor