Effect of endobronchial challenge with Actinobacillus pleuropneumoniae serotype 9 of pigs vaccinated with a vaccine containing Apx toxins and transferrin-binding proteins.
The efficacy of a subunit vaccine containing the Apx toxins of Actinobacillus pleuropneumoniae and transferrin-binding proteins was determined. Ten pigs were vaccinated twice with the vaccine. Eight control animals were injected twice with a saline solution. Three weeks after the second vaccination, all pigs were endobronchially inoculated with 10(6.5) colony-forming units (CFU) of an A. pleuropneumoniae serotype 9 strain. In the vaccine group, none of the pigs died after inoculation. Only one pig of the control group survived challenge. Surviving pigs were killed at 7 days after challenge. The mean percentage of affected lung tissue was 64% in the control group and 17% in the vaccine group. Actinobacillus pleuropneumoniae was isolated from the lungs of all animals. The mean bacterial titres of the caudal lung lobes were 5.0 x 10(8) CFU/g in the control group and 3.0 x 10(6) CFU/g in the vaccine group. It was concluded that the vaccine induced partial protection against severe challenge.
Van Overbeke
I
I
Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, University of Gent, Merelbeke, Belgium. Ingrid.Vanoverbeke@rug.ac.be
Chiers
K
K
Ducatelle
R
R
Haesebrouck
F
F
D000189:Actinobacillus Infections / Q000517:prevention & control / Q000662:veterinary*; D016977:Actinobacillus pleuropneumoniae / Q000145:classification / Q000276:immunology*; D000818:Animals; D001428:Bacterial Vaccines; D002352:Carrier Proteins; D033862:Iron-Binding Proteins; D008168:Lung / Q000382:microbiology; D018410:Pneumonia, Bacterial / Q000517:prevention & control / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control*; D033863:Transferrin-Binding Proteins; D022223:Vaccines, Subunit
D001428:Bacterial Vaccines; D002352:Carrier Proteins; D033862:Iron-Binding Proteins; D033863:Transferrin-Binding Proteins; D022223:Vaccines, Subunit