Identification and characterisation of the Drosophila homologue of the yeast Uba2 gene.
We have identified the Drosophila uba2 protein (dUba2). Analysis of the amino acid composition reveals similarity with both the mammalian (47% identity) and yeast (31% identity) homologues. dUba2 is present throughout the Drosophila life cycle but is most abundant during stages of proliferation. The protein is nucleoplasmic throughout much of the cell cycle, however it is lost from the nucleus during mitosis. The DUba2 localisation in the nucleoplasm is not uniform but is observed as concentrated patches reminiscent of the staining patterns seen for other proteins from this group. The nature of these sites is not clear, however the failure of dUba2 to localise to the sites of chorion amplification in ovaries suggests that they are not sites of ongoing DNA replication.
St Georges Hospital Medical School, Cranmer Terrace, SW17 0RE, London, UK.
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D000595:Amino Acid Sequence; D000818:Animals; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D017344:Genes, Insect; D006801:Humans; D019476:Insect Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D044764:Ubiquitin-Activating Enzymes
D029721:Drosophila Proteins; D019476:Insect Proteins; D009687:Nuclear Proteins; D044764:Ubiquitin-Activating Enzymes; C429037:uba2 protein, Drosophila