Cloning and characterization of IL-1HY2, a novel interleukin-1 family member.
The interleukin-1 (IL-1) family members play an important role in the process of inflammation and host defense. We describe here the identification and characterization of a novel member of the IL-1 family, IL-1HY2. The human IL-1HY2 protein shares significant amino acid sequence similarity (37%) with the IL-1 receptor antagonist and has a predicted three-dimensional structure similar to that of the IL-1 receptor antagonist. The IL-1HY2 gene is located in close proximity to other IL-1 family genes on human chromosome 2, and the genomic organization of the IL-1HY2 gene is highly conserved with other IL-1 family members. IL-1HY2 protein is secreted from mammalian cells, and the purified recombinant IL-1HY2 protein binds soluble IL-1 receptor type I. IL-1HY2 is expressed in human skin, spleen, and tonsil. Immunohistochemical analysis showed that the IL-1HY2 protein is expressed in the basal epithelia of skin and in proliferating B cells of the tonsil. These data suggest that IL-1HY2 is a novel IL-1 family member and that it may participate in a network of IL-1 family members to regulate adapted and innate immune responses.
Lin
H
H
Functional Genomics Department, Hyseq Inc., Sunnyvale, California 94086, USA.
Ho
A S
AS
Haley-Vicente
D
D
Zhang
J
J
Bernal-Fussell
J
J
Pace
A M
AM
Hansen
D
D
Schweighofer
K
K
Mize
N K
NK
Ford
J E
JE
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006863:Hydrogen-Ion Concentration; D007375:Interleukin-1; D007378:Interleukins / Q000737:chemistry / Q000235:genetics*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; C430241:IL1F10 protein, human; D007375:Interleukin-1; D007378:Interleukins; D011994:Recombinant Proteins