[Expression of thrombopoietin related genes in patients with idiopathic thrombocytopenic purpura].
OBJECTIVE
To explore the expression of thrombopoietin (Tpo) and its receptor c-mpl in bone marrow from patients with idiopathic thrombocytopenic purpura(ITP).


METHODS
Non-radioactive in situ hybridization was used to detect tpo, c-mpl mRNA. Immune cytochemistry was used to detect MPL on megakaryocyte.


RESULTS
The expression of tpo, c-mpl mRNA in bone marrow and MPL on megakaryocyte in patients with ITP was increased.


CONCLUSIONS
c-mpl mRNA was regulated at transcriptional level. A negative feedback loop was involved in the regulation of megakaryopoiesis in bone marrow. MPL may be involved in the regulation of circulating Tpo.
Institute of Hematology, Beijing Medical University, Beijing 100044.
X
X
Zhang
Y
Y
Yang
H
H
Shi
D001854:Bone Marrow Cells / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D008533:Megakaryocytes / Q000378:metabolism; D009363:Neoplasm Proteins; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D018121:Receptors, Cytokine; D053628:Receptors, Thrombopoietin; D013926:Thrombopoietin / Q000096:biosynthesis / Q000235:genetics*
D009363:Neoplasm Proteins; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D018121:Receptors, Cytokine; D053628:Receptors, Thrombopoietin; C082218:MPL protein, human; D013926:Thrombopoietin