Characterization of PBZ1, a probenazole-inducible gene, in suspension-cultured rice cells.
Probenazole (PBZ) induces non-race specific resistance in rice plants against rice blast fungus and PBZ1 was identified as a PBZ-inducible gene from rice. The induction of PBZ1 expression in suspension-cultured rice cells was investigated. Northern blot analysis indicated that PBZ1 was induced by PBZ in a dose-dependent manner. Enzyme-linked immunosorbent assay (ELISA) showed a dose and time-dependent accumulation of PBZ1 protein. Both mRNA and protein analysis showed that PBZ1 was not induced by salicylic acid or an active metabolite, 1,2-benzisothiazole-1,1-dioxide.
RIKEN, The Institute of Physical and Chemical Research, Wako, Saitama, Japan.
H
H
Nakashita
K
K
Yoshioka
M
M
Takayama
R
R
Kuga
N
N
Midoh
R
R
Usami
K
K
Horikoshi
K
K
Yoneyama
I
I
Yamaguchi
D002460:Cell Line; D018506:Gene Expression Regulation, Plant; D012275:Oryza / Q000737:chemistry / Q000187:drug effects / Q000235:genetics*; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D020156:Salicylic Acid / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology*
C099062:PBZ1 protein, Oryza sativa; D010940:Plant Proteins; D013844:Thiazoles; C099063:oryzemate; D020156:Salicylic Acid