Histochemical localisation of versican, aggrecan and hyaluronan in the developing condylar cartilage of the fetal rat mandible.
We investigated the histochemical localisation of versican, aggrecan and hyaluronan in the developing condylar cartilage of the fetal rat mandible at d 15-17 of gestation. At d 15 of gestation, immunostaining for versican was detected in the anlage of the future condylar process (condylar anlage), although the staining intensity showed a considerable regional variation. At d 16 of gestation, a metachromatically stained matrix firstly appeared in the condylar anlage. Aggrecan, hyaluronan and versican were simultaneously detected in this newly formed condylar cartilage. At d 17 of gestation, immunostaining for versican became restricted to the perichondrium and was barely detected in the cartilage. Colocalisation of versican and aggrecan was also seen in the cranial base cartilage at d 14 of gestation. These results indicate that although versican is replaced by aggrecan during the transition from prechondrogenic tissue to cartilage, both molecules were temporally colocalised in the newly formed cartilage. A hyaluronan-rich, low-versican area was identified in the posterior end of the condylar anlage during d 15-17 of gestation. The existence of this area is a unique structural feature of the developing condylar cartilage.
Shibata
S
S
Maxillofacial Anatomy, Department of Maxillofacial Biology, Graduate School, Tokyo Medical and Dental University, Japan. S.Shibata.oan1@dent.tmd.ac.jp
Fukada
K
K
Suzuki
S
S
Ogawa
T
T
Yamashita
Y
Y
D053674:Aggrecans; D000818:Animals; D002356:Cartilage / Q000737:chemistry* / Q000196:embryology*; D011508:Chondroitin Sulfate Proteoglycans / Q000032:analysis; D016326:Extracellular Matrix Proteins; D005865:Gestational Age; D006025:Glycosaminoglycans / Q000032:analysis*; D006651:Histocytochemistry / Q000379:methods; D006820:Hyaluronic Acid / Q000032:analysis; D037181:Lectins, C-Type; D008334:Mandible / Q000196:embryology*; D011509:Proteoglycans / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D053675:Versicans
C508321:Acan protein, rat; D053674:Aggrecans; D011508:Chondroitin Sulfate Proteoglycans; D016326:Extracellular Matrix Proteins; D006025:Glycosaminoglycans; D037181:Lectins, C-Type; D011509:Proteoglycans; C508324:Vcan protein, rat; D053675:Versicans; D006820:Hyaluronic Acid