Telomerase activity in long-standing ulcerative colitis.
Telomerase activity is frequently associated with neoplasia. It is a ribonucleoprotein capable of replacing telomeric DNA sequences that are lost at each cell division. Neoplastic progression in chronic ulcerative colitis is characterized by the development of epithelial dysplasia which is accompanied by genetic alterations. Therefore we tested telomerase activity in 128 biopsy samples of four colectomy specimens with long-standing ulcerative pancolitis by using the Telomerase PCR ELISA System. In three patients with multiple dysplastic or carcinomatous lesions, telomerase activity was detected in 22 samples with a regional association to dysplastic or carcinomatous areas. 15 of the samples with telomerase activity (68%) were found in dysplastic/carcinomatous samples or in the direct vicinity of dysplastic areas, 4 (18%), 2 positions (about 4 cm) and the remaining three (14%) not more than 3 positions away from such areas. In the fourth patient, resected because of clinical deterioration despite medical treatment and who had no dysplastic lesions, no telomerase activity was detected. These results show that telomerase activity might be used as a complementary marker to histology for the identification of patients with ulcerative colitis who are at an increased risk for neoplastic progression.
Department of Gastroenterology, University of Tuebingen, Germany. karlheinz.holzmann@uni-tuebingen.de
K
K
Holzmann
B
B
Klump
M
M
Weis-Klemm
C J
CJ
Hsieh
F
F
Borchard
M
M
Gregor
R
R
Porschen
D014408:Biomarkers, Tumor / Q000032:analysis*; D001706:Biopsy; D003082:Colectomy; D003093:Colitis, Ulcerative / Q000150:complications / Q000473:pathology* / Q000601:surgery; D003106:Colon / Q000473:pathology; D003110:Colonic Neoplasms / Q000201:enzymology / Q000209:etiology / Q000473:pathology*; D018450:Disease Progression; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D016133:Polymerase Chain Reaction; D016737:Proctocolectomy, Restorative; D019098:Telomerase / Q000032:analysis* / Q000235:genetics; D013997:Time Factors
D014408:Biomarkers, Tumor; D019098:Telomerase