Plant regeneration from leaf explants of mulberry: influence of sugar, genotype and 6-benzyladenine.
A protocol for plant regeneration from leaf explants was developed for tropical mulberry varieties. Effect of sugars, 6-benzyladenine and genotype on shoot regeneration was studied. Highest percentage of shoot regeneration (80 +/- 6) was obtained with genotype S799 on medium containing glucose and 8.9 microM 6-benzyladenine. Genotypes Mandalaya and MIHP, having thicker leaves with waxy cuticle, showed poorer regeneration ability than S799 and Sujanpur-5, which have thinner leaves and cuticle. Histological studies revealed that shoots regenerated from sub-epidermal cells.
Vijayan
K
K
Central Sericultural Research and Training Institute, Berhampore-742 101, India.
Chakraborti
S P
SP
Roy
B N
BN
D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D001593:Benzyl Compounds; D002241:Carbohydrates / Q000494:pharmacology; D005838:Genotype; D007701:Kinetin; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D011687:Purines; D012038:Regeneration / Q000187:drug effects; D019685:Rosales / Q000235:genetics / Q000254:growth & development / Q000502:physiology*
D001593:Benzyl Compounds; D002241:Carbohydrates; D010937:Plant Growth Regulators; D011687:Purines; D000225:Adenine; C480551:benzylaminopurine; D007701:Kinetin