Enhanced tolerance against salt-stress and freezing-stress of Escherichia coli cells expressing algal bbc1 gene.
The expression of the eukaryotic bbc1 (breast basic conserved) gene (the bbc1 gene of the marine green alga Chlamydomonas sp. W-80 strain) enhanced the tolerance against salt-stress and freezing-stress in E. coli cells. The expression of the BBC1 protein in the E. coli cells carrying the algal bbc1 gene and that in the Chlamydomonas W-80 cells were examined by Western blotting analysis. The result suggests that the eukaryotic BBC1 protein expressed in the E. coli cells has a protective function against the cellular dehydration.
Tanaka
S
S
The Kansai Electric Power Co, Technical Research Center, Amagasaki, Hyogo, Japan.
Ikeda
K
K
Miyasaka
H
H
D000220:Adaptation, Biological / Q000502:physiology*; D020418:Algal Proteins / Q000235:genetics / Q000502:physiology*; D000595:Amino Acid Sequence; D000818:Animals; D002696:Chlamydomonas / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D018118:Chloride Channels / Q000502:physiology; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development; D005615:Freezing; D015964:Gene Expression Regulation, Bacterial; D007038:Hypotonic Solutions; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D010957:Plasmids; D015800:Protozoan Proteins / Q000235:genetics / Q000502:physiology*; D011994:Recombinant Proteins; D012965:Sodium Chloride
D020418:Algal Proteins; D018118:Chloride Channels; D018076:DNA, Complementary; D007038:Hypotonic Solutions; D009363:Neoplasm Proteins; D015800:Protozoan Proteins; D011994:Recombinant Proteins; D012965:Sodium Chloride