[Age determination using peptide mapping of non-collagenous proteins in human dentin].
The submitted paper deals with possibilities of assessment of human age based on findings of stereospecific breakdown of proteins containing D-forms of aspartic acid. The stereospecificity of enzymatic breakdown assumes that after enzymatic hydrolysis peptide breakdown products with different molecular weights--the so-called peptide map--will be formed, depending how many D-aspartyl residues the protein contains. The authors proved in the submitted preliminary study in subjects of different age the formation of breakdown products of different size in non-collagenous proteins of human dentin which was hydrolyzed by protease V8 from Staphylococcus aureus.
Ustav biochemie a mikrobiologie, Fakulta potravinárské a biochemické technologie, VSCHT, Praha.
J
J
Sajdok
A
A
Kotrbová-Kozak
A
A
Pilin
D000328:Adult; D000366:Age Determination by Teeth / Q000379:methods*; D000368:Aged; D002851:Chromatography, High Pressure Liquid; D003804:Dentin / Q000737:chemistry*; D006801:Humans; D010449:Peptide Mapping; D010750:Phosphoproteins / Q000032:analysis*
D010750:Phosphoproteins; C017630:phosphophoryn