[Psi(+)] prion generation in yeast: characterization of the 'strain' difference.
The yeast cytoplasmically-inherited nonsense suppressor [PSI(+)] determinant is presumed to be a manifestation of the aggregated prion-like state of the Sup35 protein. Overexpression of the Sup35 protein induces generation of [PSI(+)] determinants with various suppressor efficiency and mitotic stabilities. Here, we demonstrate that the relative frequency of appearance of [PSI(+)] with different properties depends on the SUP35 allele used to induce their generation. The difference in properties of [PSI(+)] determinants was preserved after their transmission from one yeast strain to another. This difference correlated with variation in properties of the Sup35 protein. A novel type of prion instability was observed: some [PSI(+)] with weak suppressor efficiency could convert spontaneously into strong suppressor determinants.
Institute of Experimental Cardiology, Cardiology Research Center, 121552 Moscow, Russia.
N V
NV
Kochneva-Pervukhova
M B
MB
Chechenova
I A
IA
Valouev
V V
VV
Kushnirov
V N
VN
Smirnov
M D
MD
Ter-Avanesyan
D000483:Alleles; D015153:Blotting, Western; D002449:Cell Aggregation / Q000235:genetics; D002453:Cell Cycle; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal / Q000235:genetics; D010454:Peptide Termination Factors; D010641:Phenotype; D010957:Plasmids; D011328:Prions / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D013045:Species Specificity; D013489:Suppression, Genetic / Q000235:genetics*
D005656:Fungal Proteins; D010454:Peptide Termination Factors; D011328:Prions; C068068:SUP35 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins