Computer identification of Shigella species by rRNA gene restriction patterns.
We describe a MluI ribotyping scheme for Shigella which approaches correlation with serotyping. One hundred and seventeen reference strains and previously serotyped clinical isolates representing the 57 Shigella serotypes and biotypes were included in this study. A total of 51 distinct ribotypes were obtained and a database was built with them. The number of bands composing each ribotype varied from 9 to 15. The fragments ranged in size from 1.6 to 18.8 kbp. One hundred and eleven clinical isolates were successfully identified in a double blind study with standard biochemical/serologic methods, by automatic comparison of their ribotypes with our database using the software Taxotron.
Unité des entérobactéries, Inserm 389, Institut Pasteur, Paris, France.
R S
RS
Coimbra
G
G
Nicastro
P A
PA
Grimont
F
F
Grimont
D001426:Bacterial Proteins; D016208:Databases, Factual; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004405:Dysentery, Bacillary / Q000453:epidemiology / Q000382:microbiology*; D004926:Escherichia coli / Q000145:classification / Q000235:genetics; D020459:Genes, rRNA; D006801:Humans; D021521:Ribotyping; D012703:Serotyping; D012760:Shigella / Q000145:classification* / Q000235:genetics; D012984:Software
D001426:Bacterial Proteins; C035041:endodeoxyribonuclease MluI; D015252:Deoxyribonucleases, Type II Site-Specific