SPIN90 (SH3 protein interacting with Nck, 90 kDa), an adaptor protein that is developmentally regulated during cardiac myocyte differentiation.
In the yeast two-hybrid screening, we have isolated a cDNA clone from a human heart library using Nck Src homology 3 (SH3) domains as bait. The full-length cDNA, which encoded 722 amino acids, was identified as a VIP54-related gene containing an SH3 domain, proline-rich motifs, a serine/threonine-rich region, and a long C-terminal hydrophobic region. We refer to this protein as SPIN90 (SH3 Protein Interacting with Nck, 90 kDa). The amino acid sequence of the SH3 domain has the highest homology with those of Fyn, Yes, and c-Src. SPIN90 was broadly expressed in human tissues; in particular, it was highly expressed in heart, brain, and skeletal muscle, and its expression was developmentally regulated during cardiac myocyte differentiation. SPIN90 is able to bind to the first and third SH3 domains of Nck, in vitro, and is colocalized with Nck at sarcomere Z-discs within cardiac myocytes. Moreover, treatment with antisera raised against SPIN90 disrupted sarcomere structure, suggesting that this protein may play an important role in the maintenance of sarcomere structure and/or in the assembly of myofibrils into sarcomeres.
Department of Life Science, Kwangju Institute of Science and Technology, Kwangju 500-712, Korea.
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D048868:Adaptor Proteins, Signal Transducing; D000328:Adult; D000375:Aging; D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D001426:Bacterial Proteins; D001483:Base Sequence; D002454:Cell Differentiation; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D006321:Heart / Q000502:physiology*; D006801:Humans; D007381:Intermediate Filament Proteins / Q000737:chemistry; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D009206:Myocardium / Q000166:cytology*; D015513:Oncogene Proteins / Q000378:metabolism*; D009928:Organ Specificity; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012518:Sarcomeres / Q000502:physiology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D018909:src Homology Domains
D048868:Adaptor Proteins, Signal Transducing; D001426:Bacterial Proteins; D007381:Intermediate Filament Proteins; D009124:Muscle Proteins; C486062:NCKIPSD protein, human; C078332:Nck protein; D015513:Oncogene Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; C404157:VIP54 protein, Helicobacter pylori