[The growth effect of granulocyte colony-stimulating factors on ovarian carcinoma cell lines].
OBJECTIVE
To evaluate the growth effects of granulocyte colony-stimulating factors (G-CSF) on ovarian carcinoma cell lines.


METHODS
We investigated the possible growth effects of G-CSF on three ovarian carcinoma cell lines (SKOV3, 3AO, and CAOV3). Cell proliferation and viability was measured by XTT colorimetric assay. Flow cytometer (FCM) and G-CSF receptor mRNA were measured for response cell lines, the response cell lines were xenotransplanted into athymic mice, to investigated in vivo growth under systemic subcutaneous infusion of G-CSF.


RESULTS
G-CSF showed no growth-stimulating effects in 3AO and CAOV3 cell lines. In SKOV3, an increase in growth (< 5%) was seen by XTT assay at 100 ng/ml and 1000 ng/ml after 24 hours treatment, but no significant proliferation was observed by FCM. No G-CSF receptor mRNA was detected by RT-polymerase chain reaction, and no significant alteration of the tumor growth was by G-CSF.


CONCLUSIONS
Although, the above results suggest that G-CSF may act as growth factors in a few ovarian carcinoma cell lines, yet the worry about tumor growth stimulation by G-CSF as potential hazards for their clinical application in ovarian carcinoma patients as an adjuvant with cytotoxic chemotherapy is not necessary.
General Hospital of Beijing Military Area, Beijing 100700.
N
N
Wu
H
H
Cui
H
H
Qian
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D018283:Cystadenocarcinoma, Papillary / Q000473:pathology*; D018284:Cystadenocarcinoma, Serous / Q000473:pathology; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010051:Ovarian Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured
D016179:Granulocyte Colony-Stimulating Factor