[Detection of mycobacterium tuberculosis by AmpliSensor-PCR technique and its clinical application].
OBJECTIVE
To explore the value of Amplisensor-PCR method in diagnosis of tuberculosis.


METHODS
The samples from 784 patients with tuberculosis, 160 patients with lung cancer were detected by using AmpliSensor-PCR, PCR, smear and culture methods, and the results were compared.


RESULTS
The sensitivity of AmpliSensor-PCR technique was obviously higher than that of smear and culture (P < 0.01). The specificity of AmpliSensor-PCR method was higher than that of PCR method.


CONCLUSIONS
Amplisensor-PCR method can be determined to detect the lower limit by standard curve, through which the original amount of target-DNA in samples can be calculated. AmpliSensor-PCR method shows higher specificity and sensitivity. It is of clinical value in diagnosis of pulmonary tuberculosis and particularly of extrapulmonary tuberculosis.
Pulmonary Disease Institute, Guangzhou Chest Hospital, Guangzhou 510095.
Y
Y
Tan
Y
Y
Li
S
S
Tan
D004269:DNA, Bacterial / Q000032:analysis*; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods; D014390:Tuberculosis, Meningeal / Q000382:microbiology; D014396:Tuberculosis, Pleural / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000382:microbiology*
D004269:DNA, Bacterial