The potential of nucleic acid repair in functional genomics.
Chimeric RNA/DNA oligonucleotides have been used successfully to correct point and frameshift mutations in cells as well as in animal and plant models. This approach is one of several nucleic acid repair technologies that will help elucidate the function of newly discovered genes. Understanding the mechanisms by which these different technologies direct gene alteration is essential for progress in their application to functional genomics.
Department of Biological Science, University of Delaware, Newark, DE 19716, USA.
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D004247:DNA / Q000737:chemistry; D004249:DNA Damage; D004260:DNA Repair; D016678:Genome; D008957:Models, Genetic; D009696:Nucleic Acids / Q000737:chemistry*; D009841:Oligonucleotides / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D012313:RNA / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000378:metabolism
D009696:Nucleic Acids; D009841:Oligonucleotides; D011993:Recombinant Fusion Proteins; D012313:RNA; D004247:DNA