Stability indicating HPTLC determination of Trimetazidine as bulk drug and in pharmaceutical formulations.
A simple, selective, precise and stability-indicating high-performance thin-layer chromatographic method of analysis of trimetazidine hydrochloride both as a bulk drug and in formulations is reported. The mobile phase composition was n-butanol-water-methanol-ammonia (20%) (14:0.2:0.2:2, v/v/v/v). Densitometric analysis of trimetazidine hydrochloride was carried out in the absorbance mode at 254 nm. the calibration curve of trimetazidine hydrochloride in methanol was linear in the range 400 -- 2400 ng. The mean value of correlation coefficient, slope and intercept were 0.99815 and #61617;0.001, 0.4849 and #61617;0.001 and 31.633 and #61617;5.996 respectively. The limits of detection and quantitation were 50 and 80 ng respectively. The recovery of trimetazidine hydrochloride was about 98 -- 100%. This method was utilized to analyze trimetazidine hydrochloride from conventional tablets and controlled release pellets in the presence if commonly used excipients.
Pharmaceutical Division, Department of Chemical Technology (Autonomous), University of Mumbai, Nathalal Parikh Marg, Matunga, Mumbai 400 019, India.
S O
SO
Thoppil
R M
RM
Cardoza
P D
PD
Amin
D002138:Calibration; D002626:Chemistry, Pharmaceutical; D002855:Chromatography, Thin Layer / Q000379:methods*; D011786:Quality Control; D015203:Reproducibility of Results; D014292:Trimetazidine / Q000032:analysis*; D014665:Vasodilator Agents / Q000032:analysis*
D014665:Vasodilator Agents; D014292:Trimetazidine