Structure and characterization of the human insulin-like growth factor binding protein (IGFBP)-6 promoter: identification of a functional retinoid response element.
The 1.7 kb human insulin-like growth factor binding protein (IGFBP)-6 gene 5'-flanking region was sequenced and found to have promoter activity in human osteoblasts. The sequence contains four clustered transcription start sites and three retinoic acid response elements (RAREs) with widely spaced half-sites. Only the proximal DR15 RARE was functional. Retinoids increased IGFBP-6 promoter activity up to 3-fold.
Musculoskeletal Disease Center, J.L. Pettis Memorial Veterans' Medical Center, 11201 Benton Street, Loma Linda, CA 92357, USA.
Y P
YP
Dailly
Y
Y
Zhou
T A
TA
Linkhart
D J
DJ
Baylink
D D
DD
Strong
D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary; D006801:Humans; D018976:Insulin-Like Growth Factor Binding Protein 6 / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D010006:Osteoblasts; D011401:Promoter Regions, Genetic; D051381:Rats; D020218:Response Elements; D012176:Retinoids / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D018976:Insulin-Like Growth Factor Binding Protein 6; D012176:Retinoids