Influence of batroxobin on cerebral ischemia-reperfusion injury in gerbils.
OBJECTIVE
To study the effects of batroxobin (Bat) on neurons survival, neurobehavioral test, ATP levels and hydroxyl radical outputs in hippocampus during forebrain ischemia-reperfusion in gerbils.


METHODS
The forebrain ischemia was induced by occluding the bilateral common carotid arteries for 10 min in gerbils, and ATP levels and 2, 3-dihydroxybenzoic acid (DHBA) outputs were assayed by HPLC. The neurons survival were assessed by histology, and behavioral tests of gerbils were assessed by open field test.


RESULTS
The number of neurons survival in Ir at d 7 postischemic insult were (7 +/- 4)% of sham-operated gerbils, much less than that in Bat (45 +/- 16)%. The levels of explore activities of ischemic gerbils was 175% and 159% of sham-operated gerbils at d 3 and d 6 postischemic insult, much more than that in Bat (120% d 3 and 140% d 6). Hippocampal ATP levels in Ir were 64% of sham-operated gerbils at reperfusion 60 min, much less than that in Bat I and II (82% and 89% respectively). The hippocampal 2,3-DHBA outputs in Ir increased by 4.5 folds of sham-operated gerbils at reperfusion 60 min, but the 2,3-DHBA outputs in Bat I and Bat II were only 2.6 and 2.4 folds respectively.


CONCLUSIONS
Bat possesses the inhibitory effects on DND and OH. production following cerebral ischemia-reperfusion in gerbils.
Chen
Q
Q
Department of Anesthesiology, Affiliated Hospital of Xuzhou Medical College, China. xmcfa@cumt.edu.cn
Zeng
Y M
YM
Xu
P C
PC
Fan
J W
JW
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D012103:Batroxobin / Q000494:pharmacology*; D002545:Brain Ischemia / Q000150:complications; D002470:Cell Survival / Q000187:drug effects; D005343:Fibrinolytic Agents / Q000494:pharmacology; D005849:Gerbillinae; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D062385:Hydroxybenzoates / Q000378:metabolism; D008297:Male; D018782:Maze Learning; D009474:Neurons / Q000187:drug effects; D015427:Reperfusion Injury / Q000209:etiology / Q000378:metabolism* / Q000473:pathology
D005343:Fibrinolytic Agents; D062385:Hydroxybenzoates; C009135:2,3-dihydroxybenzoic acid; D000255:Adenosine Triphosphate; D012103:Batroxobin