High level excretion of Norwalk-like virus following resolution of clinical illness.
We report the case of an elderly woman excreting high levels (about 5 x 10(5) virions per gram of faeces) of Norwalk-like virus (NLV) in the absence of any clinical symptoms of gastroenteritis. Analysis by reverse transcription, polymerase chain reaction and DNA sequencing was carried out on a 342-nucleotide region of open reading frame 1. This indicated that the NLV belonged to genogroup 2 and was more closely related to the Camberwell subgroup, the most common circulating in southeast Australia at present, than to the Norwalk and Mexico viruses.
Marshall
J A
JA
Victorian Infectious Diseases Reference Laboratory, Carlton South, Australia. john.marshall@mh.org.au
Salamone
S
S
Yuen
L
L
Catton
M G
MG
Wright
J P
JP
D000368:Aged; D017250:Caliciviridae Infections / Q000821:virology*; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis; D005243:Feces / Q000821:virology*; D005260:Female; D005759:Gastroenteritis / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D009663:Norwalk virus / Q000145:classification / Q000302:isolation & purification* / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017201:Virus Shedding
D017931:DNA Primers; D004279:DNA, Viral