Embryonic, fetal, and neonatal tongue myoblasts exhibit molecular heterogeneity in vitro.
Variable gene expression patterns have been shown to exist between embryonic, fetal, and neonatal lineages of limb skeletal myoblasts in vitro and in vivo. In this study, we examined the molecular phenotype of embryonic, fetal, and neonatal tongue myoblasts in primary culture for comparison with in vivo developmental tongue myoblasts. Myogenic regulatory factor (MRF) and myosin heavy chain (MHC) gene expression were determined in culture during both growth and differentiation conditions by PCR, immunoblotting, and immunohistochemistry. Unlike their in vivo tongue myoblast equivalents, developmental tongue myoblast cultures featured the expression of MyoD when kept in growth conditions. Differentiation conditions in vitro induced myogenic tongue lineages to maintain characteristics of their in vivo morphologic and contractile gene phenotype. Both in vivo and in vitro, embryonic tongue lineages predominantly expressed MHC-embryonic isoforms, while fetal and neonatal tongue lineages predominantly expressed fast and perinatal isoforms of contractile genes. A notable difference from the in vivo condition that was observed in differentiated tongue myotubes in vitro was the presence of the MHC-slow protein. It was previously demonstrated that MHC-slow protein was undetectable during the in vivo development of the tongue musculature despite the abundance of slow isoform transcripts. The present characterization of primary tongue myogenic cultures indicates that murine myoblast heterogeneity exists primarily between developmental lineages at the level of contractile gene expression. Outside their native surroundings, developmental myogenic tongue populations are unable to recapitulate the determination and differentiation molecular profiles that occur in vivo.
Dalrymple
K R
KR
Center for Craniofacial Molecular Biology, School of Dentistry, University of Southern California, 2250 Alcazar, CSA 103, Los Angeles, CA 90033, USA.
Prigozy
T I
TI
Shuler
C F
CF
D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D019070:Cell Lineage; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005260:Female; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D009119:Muscle Contraction / Q000235:genetics; D024510:Muscle Development; D018656:Muscle Fibers, Fast-Twitch / Q000378:metabolism / Q000502:physiology; D018657:Muscle Fibers, Slow-Twitch / Q000378:metabolism / Q000502:physiology; D018482:Muscle, Skeletal / Q000166:cytology / Q000254:growth & development* / Q000502:physiology; D018006:Myogenic Regulatory Factors / Q000096:biosynthesis / Q000235:genetics; D018995:Myosin Heavy Chains / Q000096:biosynthesis / Q000235:genetics; D010641:Phenotype; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014059:Tongue / Q000166:cytology / Q000254:growth & development* / Q000502:physiology
D018006:Myogenic Regulatory Factors; D018995:Myosin Heavy Chains