Prevention of glucoprivic stimulation of corticosterone secretion by leptin does not restore high frequency luteinizing hormone pulses in rats.
We have previously determined that exogenous leptin prevents the inhibition of pulsatile luteinizing hormone (LH) release in the fasting rodent. The present study tested the hypothesis that the mechanism by which leptin facilitates high LH secretion is through an attenuation of the stress response produced by a deficit in energy. Because hypogonadotropism is associated with activation of the hypothalamic-pituitary-adrenal (HPA) axis during both metabolic stress and nonmetabolic stress, our approach included a comparison of whether exogenous leptin could prevent the rise in corticosterone produced by a nonmetabolic stress (immobilization for 2 h), as well as by a widely used metabolic stress (transient glucoprivation by 2-deoxyglucose, 2DG; 400 mg/kg, b.w., i.v.). Each stressor was applied to well-fed ovariectomized rats (n = 4-6 per group), 2 h after leptin (3 microg/g, b.w., i.p.) or vehicle administration. Blood samples were collected through an indwelling atrial cannula every 6 min for 1 h before and for 2 h after the stress treatment to measure LH, leptin and corticosterone. During metabolic stress (acute glucoprivation), circulating leptin decreased, corticosterone increased and LH decreased; leptin administration abolished the increase in corticosterone, but pulsatile LH secretion remained inhibited. In contrast, during nonmetabolic stress (immobilization), leptin secretion was unaffected, but circulating corticosterone increased and LH decreased; leptin treatment did not prevent either the increase in corticosterone or the decrease in LH secretion. An important overall finding is that leptin can differentially alter the HPA axis depending upon the type of stress. In addition, whether the pattern of leptin is altered depends upon the type of stress. Although a glucoprivic-induced decrease in endogenous leptin can be a stressor responsible for the increase in corticosterone secretion, a nonmetabolic stress-induced increase in corticosterone is not mediated by leptin. Moreover, our results reveal that the depression of LH secretion when leptin is low during reduced energy availability is not due to activation of the HPA axis. During an energy deficit, exogenous leptin could not restore high frequency LH secretion when HPA function was restored to normal. Finally, the inability of leptin to increase LH secretion in the face of 2DG supports the notion that the action of leptin is dependent upon the degree of glucose availability.
Nagatani
S
S
Reproductive Sciences Program, University of Michigan, Ann Arbor, MI 48109-0404, USA.
Thompson
R C
RC
Foster
D L
DL
D000818:Animals; D003345:Corticosterone / Q000097:blood*; D003847:Deoxyglucose / Q000494:pharmacology; D005260:Female; D005947:Glucose / Q000037:antagonists & inhibitors* / Q000378:metabolism; D007030:Hypothalamo-Hypophyseal System / Q000187:drug effects / Q000378:metabolism; D020738:Leptin / Q000378:metabolism* / Q000494:pharmacology; D007986:Luteinizing Hormone / Q000097:blood*; D010052:Ovariectomy; D010913:Pituitary-Adrenal System / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012149:Restraint, Physical; D013312:Stress, Physiological / Q000097:blood
D020738:Leptin; D007986:Luteinizing Hormone; D003847:Deoxyglucose; D005947:Glucose; D003345:Corticosterone