Production of human type I collagen in yeast reveals unexpected new insights into the molecular assembly of collagen trimers.
Substantial evidence supports the role of the procollagen C-propeptide in the initial association of procollagen polypeptides and for triple helix formation. To evaluate the role of the propeptide domains on triple helix formation, human recombinant type I procollagen, pN-collagen (procollagen without the C-propeptides), pC-collagen (procollagen without the N-propeptides), and collagen (minus both propeptide domains) heterotrimers were expressed in Saccharomyces cerevisiae. Deletion of the N- or C-propeptide, or both propeptide domains, from both proalpha-chains resulted in correctly aligned triple helical type I collagen. Protease digestion assays demonstrated folding of the triple helix in the absence of the N- and C-propeptides from both proalpha-chains. This result suggests that sequences required for folding of the triple helix are located in the helical/telopeptide domains of the collagen molecule. Using a strain that does not contain prolyl hydroxylase, the same folding mechanism was shown to be operative in the absence of prolyl hydroxylase. Normal collagen fibrils were generated showing the characteristic banding pattern using this recombinant collagen. This system offers new opportunities for the study of collagen expression and maturation.
Olsen
D R
DR
Cohesion Technologies Inc., Palo Alto, California 94303, USA.
Leigh
S D
SD
Chang
R
R
McMullin
H
H
Ong
W
W
Tai
E
E
Chisholm
G
G
Birk
D E
DE
Berg
R A
RA
Hitzeman
R A
RA
Toman
P D
PD
D002942:Circular Dichroism; D003094:Collagen / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D017364:Collagenases / Q000737:chemistry; D010450:Endopeptidases / Q000737:chemistry; D006801:Humans; D008854:Microscopy, Electron; D011347:Procollagen / Q000235:genetics; D017510:Protein Folding; D020836:Protein Structure, Quaternary; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017384:Sequence Deletion
D011347:Procollagen; D003094:Collagen; D010450:Endopeptidases; D017364:Collagenases