Polysaccharide hydrolases of Aureobasidium pullulans.
The polysaccharide hydrolase activity of a group of selected strains of the genus Aureobasidium pullulans was investigated using a new gel testing assay. A total of 31 strains were tested for alpha-amylase, alpha-glucosidase and glucoamylase, beta-glucosidase, lichenase, cellulase, xylanase and xylosidase, mannanase and mannosidase production during growth of microorganisms on respective meshed polysaccharide gels. Attempts were made to increase the polysaccharide hydrolase activity through selection of some A. pullulans strains by passaging them on the respective modified xylanase- and cellulase-containing gels. The individual saccharide degradation cleavage products were investigated by chromatography.
Augustín
J
J
Department of Milk, Fats and Food Hygiene, Faculty of Chemical Technology, Slovak Technical University, 812 37 Bratislava, Slovakia.
D002480:Cellulase / Q000378:metabolism; D002850:Chromatography, Gel; D006867:Hydrolases / Q000378:metabolism*; D003904:Mitosporic Fungi / Q000201:enzymology*; D011134:Polysaccharides / Q000378:metabolism*; D043325:Xylan Endo-1,3-beta-Xylosidase; D014995:Xylosidases / Q000378:metabolism; D000516:alpha-Amylases / Q000378:metabolism
D011134:Polysaccharides; D006867:Hydrolases; D014995:Xylosidases; D000516:alpha-Amylases; D043325:Xylan Endo-1,3-beta-Xylosidase; D002480:Cellulase