Identification of lectin isoforms in juvenile freshwater prawns Macrobrachium rosenbergii (DeMan, 1879).
From the serum of juvenile freshwater prawns, we isolated by affinity chromatography on glutaraldehyde-fixed rat erythrocytes stroma, immobilized in Sephadex G-25, a sialic acid specific lectin of 9.6 kDa per subunit. Comparative analysis against adult organisms purified lectin, by chromatofocusing, showed that the lectin from juvenile specimens is composed by four main isoforms with a pI of 4.2, 4.6, 5.1, and 5.6, whereas the lectin from adults is eluted at pH 4.2. The amino acid composition of the lectin obtained from adult and juvenile stages suggest identity, but the compositions are not identical since a higher content of carbohydrates was found in the lectin from younger organisms. The freshwater prawn lectin showed specificity toward N-acetylated amino sugar residues such as GlcNAc, GalNAc, Neu5Ac and Neu5,9Ac; but in juvenile organisms the lectin showed three times less hemagglutinating activity than the lectin from adults. Both lectins agglutinated rat, rabbit and chicken erythrocytes, indicating that Neu5,9Ac in specific O-glycosydically linked glycans seems to be relevant for the interaction of M. rosenbergii lectins with their specific cellular receptor. Our results suggest that the physicochemical characteristics of the lectin from the freshwater prawn are regulated through maturation.
Zenteno
R
R
Departamento de Bioquímica, Instituto Nacional de Enfermedades Respiratorias, Calzada de Tlalpan, Mexico. ezenteno@servidor.unam.mx
Vázquez
L
L
Martínez-Cairo
S
S
Bouquelet
S
S
Agundis
C
C
Zenteno
E
E
D000372:Agglutination Tests; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000032:analysis; D002645:Chickens; D003445:Crustacea / Q000737:chemistry* / Q000254:growth & development; D004912:Erythrocytes / Q000187:drug effects; D005618:Fresh Water; D006458:Hemolymph / Q000737:chemistry; D006801:Humans; D037102:Lectins / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D011817:Rabbits; D051381:Rats; D013045:Species Specificity; D013379:Substrate Specificity
D000596:Amino Acids; D002241:Carbohydrates; D037102:Lectins