Isolation of monoclonal antibodies that inhibit the binding of infectious bursal disease virus to LSCC-BK3 cells.
Three hybridoma cell lines producing monoclonal antibodies (MAbs) against LSCC-BK3 cells which are susceptible to infectious bursal disease virus (IBDV) infection were produced and characterized. The MAbs, designated T7, Q11 and Q13, inhibited the attachment of IBDV to LSCC-BK3 cells. Furthermore, these MAbs bound to LSCC-BK3 but not to nonpermissive cells in flow cytometry. MAb T7 detected a 110-kDa membrane protein of LSCC-BK3 cells, whereas Q11 and Q13 reacted with membrane proteins of molecular weights 58-, 85-, 90- and 110-kDa. These observations imply that the 110-kDa protein recognized by all the MAbs is associated with IBDV binding. The MAbs established in this study are useful for studying the interaction between IBDV and its target cell.
Setiyono
A
A
Department of Veterinary Microbiology, Faculty of Agriculture, Gifu University, Japan.
Yamaguchi
T
T
Ogawa
M
M
Fukushi
H
H
Hirai
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification; D000956:Antigens, Viral / Q000276:immunology; D018175:Birnaviridae Infections / Q000276:immunology / Q000662:veterinary*; D015153:Blotting, Western / Q000662:veterinary; D002478:Cells, Cultured; D002645:Chickens; D005434:Flow Cytometry / Q000662:veterinary; D006825:Hybridomas / Q000276:immunology / Q000378:metabolism; D007243:Infectious bursal disease virus / Q000276:immunology* / Q000378:metabolism; D008565:Membrane Proteins / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D011201:Poultry Diseases / Q000276:immunology* / Q000821:virology*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D008565:Membrane Proteins