Molecular cloning and characterization of a novel mammalian endo-apyrase (LALP1).
Here we describe the cloning, localization, and characterization of a novel mammalian endo-apyrase (LALP1) in human and mouse. The predicted human LALP1 gene encodes a 604-amino acid protein, whereas the mouse Lalp1 gene encodes a 606-amino acid protein. The human and mouse genes have 88% amino acid sequence identity. These genes share considerable homologies with hLALP70, a recently discovered mammalian lysosomal endo-apyrase. The human LALP1 gene resides on chromosome 10q23-q24 and contains 12 exons and 11 introns covering a genomic region of approximately 46 kilobase pairs. The subcellular localization and enzymatic activity of LALP1 indicated that LALP1 is indeed an endo-apyrase with substrate preference for nucleoside triphosphates UTP, GTP, and CTP.
Shi
J D
JD
Department of Pathology, Immunology and Laboratory Medicine, Center for Mammalian Genetics and Diabetes Center of Excellence, College of Medicine, University of Florida, Gainesville, Florida 32610, USA.
Kukar
T
T
Wang
C Y
CY
Li
Q Z
QZ
Cruz
P E
PE
Davoodi-Semiromi
A
A
Yang
P
P
Gu
Y
Y
Lian
W
W
Wu
D H
DH
She
J X
JX
D000595:Amino Acid Sequence; D000818:Animals; D001081:Apyrase / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10; D003001:Cloning, Molecular; D003570:Cytidine Triphosphate / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008297:Male; D008322:Mammals; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011755:Pyrophosphatases / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D014162:Transfection; D014544:Uridine Triphosphate / Q000378:metabolism
D011994:Recombinant Proteins; D003570:Cytidine Triphosphate; D006160:Guanosine Triphosphate; D011755:Pyrophosphatases; D001081:Apyrase; C429540:ENTPD7 protein, human; C486105:Lysal2 protein, mouse; D014544:Uridine Triphosphate