Defined sequence segments of the small heat shock proteins HSP25 and alphaB-crystallin inhibit actin polymerization.
The interaction of small heat shock proteins (sHSPs) with the actin cytoskeleton has been described and some members of this family, e.g. chicken and murine HSP25 (HSP27), inhibit the polymerization of actin in vitro. To analyse the molecular basis of this interaction, we synthesized a set of overlapping peptides covering the complete sequence of murine HSP25 and tested the effect of these peptides on actin polymerization in vitro by fluorescence spectroscopy and electron microscopy. Two peptides comprising the sequences W43 to R57 (peptide 6) and I92 to N106 (peptide 11) of HSP25 were found to be potent inhibitors of actin polymerization. Phosphorylation of N-terminally extended peptide 11 at serine residues known to be phosphorylated in vivo resulted in decline of their inhibitory activity. Interestingly, peptides derived from the homologous peptide 11 sequence of murine alphaB-crystallin showed the same behaviour. The results suggest that both HSP25 and alphaB-crystallin have the potential to inhibit actin polymerization and that this activity is regulated by phosphorylation.
Max Delbrück Center of Molecular Medicine, Berlin, Germany.
M
M
Wieske
R
R
Benndorf
J
J
Behlke
R
R
Dölling
G
G
Grelle
H
H
Bielka
G
G
Lutsch
D000199:Actins / Q000037:antagonists & inhibitors*; D000595:Amino Acid Sequence; D000818:Animals; D016384:Consensus Sequence; D017124:Conserved Sequence; D003459:Crystallins / Q000737:chemistry / Q000494:pharmacology*; D006360:Heat-Shock Proteins; D051379:Mice; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D018832:Molecular Chaperones; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000737:chemistry / Q000494:pharmacology* / Q000648:ultrastructure; D010446:Peptide Fragments / Q000494:pharmacology; D010766:Phosphorylation; D011108:Polymers; D016415:Sequence Alignment; D012694:Serine / Q000378:metabolism; D013329:Structure-Activity Relationship
D000199:Actins; D003459:Crystallins; D006360:Heat-Shock Proteins; C486113:Hsbp1 protein, mouse; D018832:Molecular Chaperones; D009363:Neoplasm Proteins; D010446:Peptide Fragments; D011108:Polymers; D012694:Serine