Prevention of adhesions by surfactants and cellulose derivatives in mice.
OBJECTIVE
To evaluate the efficacy of hydrophobically modified ethyl (hydroxyethyl) cellulose (cellulose), sodium hyaluronate (hyaluronate) and phosphatidylglycerol, in the reduction of adhesion formation.


METHODS
Controlled study.


METHODS
Experimental academic unit, Sweden.


METHODS
NMRI mice. Solutions: (1) cellulose, (2) hyaluronate, (3) phosphatidylglycerol, (4) phosphatidylglycerol and cellulose, and (5) phosphatidylglycerol, cellulose and hyaluronate.


METHODS
A standard lesion was created in the parietal peritoneum in mice. One of the viscous solutions to be tested, or saline, was given intraperitoneally.


METHODS
Amount of adhesions found one week postoperatively.


RESULTS
Cellulose; phosphatidylglycerol and cellulose; and phosphatidylglycerol, cellulose and hyaluronate all significantly reduced the amount of adhesions (p=0.0002, p=0.002, p < 0.0001), as did the hyaluronate alone (p < 0.05). Phosphatidylglycerol alone did not reduce the amount of adhesions. Combining cellulose with phosphatidylglycerol, or with hyaluronate, did not improve efficacy.


CONCLUSIONS
Cellulose and hyaluronate were effective in reducing the formation of adhesions. Combining cellulose with hyaluronate or phosphatidylglycerol or both did not improve efficacy.
Falk
K
K
Department of Surgery, Sahlgrenska University Hospital, Göteborg University, Sweden.
Lindman
B
B
Bengmark
S
S
Larsson
K
K
Holmdahl
L
L
D000818:Animals; D002482:Cellulose / Q000494:pharmacology*; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D006821:Hyaluronoglucosaminidase / Q000494:pharmacology*; D007274:Injections, Intraperitoneal; D007813:Laparotomy / Q000009:adverse effects* / Q000379:methods; D051379:Mice; D008815:Mice, Inbred Strains; D010715:Phosphatidylglycerols / Q000494:pharmacology*; D011183:Postoperative Complications / Q000517:prevention & control; D011336:Probability; D012016:Reference Values; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric; D013501:Surface-Active Agents / Q000494:pharmacology*; D000267:Tissue Adhesions / Q000517:prevention & control*
D010715:Phosphatidylglycerols; D013501:Surface-Active Agents; D002482:Cellulose; D006821:Hyaluronoglucosaminidase