On-line, continuous and automatic monitoring of extracellular malondialdehyde concentration in anesthetized rat brain cortex.
An assay for in vivo, continuous and automatic monitoring of extracellular malondialdehyde concentrations in anesthetized rat brain cortex was developed. This method involved the use of microdialysis perfusion, on-line derivatization and on-line high-performance liquid chromatographic analysis. Microdialysate from an implanted microdialysis probe was on-line reacted with thiobarbituric acid at 80 degrees C for 10 min prior to on-line collection and automatic injection into a HPLC system equipped with a fluorescence detector. This method gave a linear response between the concentrations of the malondialdehyde in the microdialysates and the TEP solution where the microdialysis probe was placed. This method was used to observe the increased extracellular malondialdehyde production following elevated extracellular glutamate levels, which were achieved by perfusion of L-trans-pyrrolidine-2,4-dicarboxylate, a competitive inhibitor of glutamate uptake transporter.
Yang
C S
CS
Department of Education and Research, Taichung Veterans General Hospital, Taiwan. csymdr@vghtc.vghtc.gov.tw
Tsai
P J
PJ
Chen
W Y
WY
Kuo
J S
JS
D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003998:Dicarboxylic Acids / Q000737:chemistry; D005110:Extracellular Space / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism*; D017551:Microdialysis; D011759:Pyrrolidines / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D013050:Spectrometry, Fluorescence
D003998:Dicarboxylic Acids; D011759:Pyrrolidines; C103758:pyrrolidine-2,3-dicarboxylic acid; D018698:Glutamic Acid; D008315:Malondialdehyde