Genomic diversity of Bartonella henselae isolates from domestic cats from Japan, the USA and France by pulsed-field gel electrophoresis.
The genomic DNA diversity of 27 Bartonella henselae and three B. clarridgeiae isolates from 18 domestic cats from Japan, the USA and France was investigated by pulsed-field gel electrophoresis (PFGE) with NotI, AscI and SmaI restriction enzymes. A great diversity of genomic patterns was found for all B. henselae, but none for B. clarridgeiae isolates. The DNA size of B. henselae and B. clarridgeiae isolates were 1.7-2.9 and 1.7Mbp, respectively. All 13 Japanese cat isolates were identified as B. henselae type I. Furthermore, three of the four Japanese cats harbored genetically different B. henselae type I isolates, suggesting for the first time co-infection with various type I isolates. One French cat and one American cat were co-infected with B. henselae and B. clarridgeiae. B. henselae type I and type II were mainly grouped in two different clusters by PFGE using SmaI endonuclease in the dendrogram.
Maruyama
S
S
Laboratory of Veterinary Public Health, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, 252-8510, Kanagawa, Japan. maruyama@brs.nihon-u.ac.jp
Kasten
R W
RW
Boulouis
H J
HJ
Gurfield
N A
NA
Katsube
Y
Y
Chomel
B B
BB
D000818:Animals; D018416:Bartonella henselae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D002371:Cat Diseases / Q000382:microbiology*; D002372:Cat-Scratch Disease / Q000382:microbiology / Q000662:veterinary*; D002415:Cats; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D005602:France; D014644:Genetic Variation / Q000235:genetics; D016680:Genome, Bacterial; D007564:Japan; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D014481:United States
D004269:DNA, Bacterial