Herpes simplex virus type 1 promoter activity during latency establishment, maintenance, and reactivation in primary dorsal root neurons in vitro.
A neonatal rat dorsal root ganglion-derived neuronal culture system has been utilized to study herpes simplex virus (HSV) latency establishment, maintenance, and reactivation. We present our initial characterization of viral gene expression in neurons following infection with replication-defective HSV recombinants carrying beta-galactosidase and/or green fluorescent protein reporter genes under the control of lytic cycle- or latency-associated promoters. In this system lytic virus reporter promoter activity was detected in up to 58% of neurons 24 h after infection. Lytic cycle reporter promoters were shut down over time, and long-term survival of neurons harboring latent virus genomes was demonstrated. Latency-associated promoter-driven reporter gene expression was detected in neurons from early times postinfection and was stably maintained in up to 83% of neurons for at least 3 weeks. In latently infected cultures, silent lytic cycle promoters could be activated in up to 53% of neurons by nerve growth factor withdrawal or through inhibition of histone deacetylases by trichostatin A. We conclude that the use of recombinant viruses containing reporter genes, under the regulation of lytic and latency promoter control in neuronal cultures in which latency can be established and reactivation can be induced, is a potentially powerful system in which to study the molecular events that occur during HSV infection of neurons.
Division of Virology, Department of Pathology, University of Cambridge, Cambridge, United Kingdom. jla@mole.bio.cam.ac.uk
J L
JL
Arthur
C G
CG
Scarpini
V
V
Connor
R H
RH
Lachmann
A M
AM
Tolkovsky
S
S
Efstathiou
D000818:Animals; D000831:Animals, Newborn; D002460:Cell Line; D002470:Cell Survival; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics; D005727:Ganglia, Spinal / Q000187:drug effects / Q000821:virology*; D015967:Gene Expression Regulation, Viral / Q000187:drug effects; D017930:Genes, Reporter / Q000235:genetics; D018259:Herpesvirus 1, Human / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D006877:Hydroxamic Acids / Q000494:pharmacology; D020932:Nerve Growth Factor / Q000494:pharmacology; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000821:virology*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014775:Virus Activation / Q000187:drug effects / Q000235:genetics*; D017735:Virus Latency / Q000187:drug effects / Q000235:genetics*
D004274:DNA, Recombinant; D006877:Hydroxamic Acids; C012589:trichostatin A; D020932:Nerve Growth Factor