Formation of myo-inositol phosphates by Aspergillus niger 3-phytase.
Kinetics of phytate hydrolysis by Aspergillus niger phytase and correlation between the amount of released phosphate and creation of lower myo-inositol phosphates were investigated. Phytase was able to hydrolyze myo-inositol hexakis-, pentakis-, tetrakis-, and trisphosphates. Finally, about 56% of total phosphate were released and myo-inositol bisphosphate was detected as the end-product.
Laboratory of Biotransformation, Institute of Microbiology, Academy of Sciences of the Czech Republic, 142 20 Prague, Czechia.
J
J
Dvoráková
J
J
Kopecký
V
V
Havlícek
V
V
Kren
D001234:Aspergillus niger / Q000201:enzymology*; D002851:Chromatography, High Pressure Liquid; D007295:Inositol Phosphates / Q000032:analysis / Q000378:metabolism*; D007700:Kinetics; D013058:Mass Spectrometry; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D010833:Phytic Acid / Q000378:metabolism
D007295:Inositol Phosphates; D010833:Phytic Acid; D010744:Phosphoric Monoester Hydrolases; C400321:3-phytase