Effects of serum from patients with type 1 diabetes on primary cerebellar granule cells.
Type 1 diabetes is an autoimmune disease of unknown etiology. Our previous work has shown that a factor present in serum from type 1 diabetic patients causes increased Ca2+ channel activity and apoptotic DNA fragmentation in pancreatic beta-cells. Here we examined the effects of type 1 diabetic serum on primary cerebellar granule cells (CGCs). In CGCs, exposure to type 1 diabetic serum did not cause increased apoptosis or changes in Ca2+ channel activity. However, patient serum did cause modulation of Ca2+ signals in a cell type with triangular soma that exhibited low voltage-gated Ca2+ currents. This cell was present primarily in cultures exposed to type 1 diabetic serum. The presence of low voltage-gated Ca2+ currents and long neuronal dendrites indicated that this unique cell was of neuronal origin and not of glial origin.
Division of Toxicology, Institute for Environmental Medicine, Rolf Luft Center for Diabetes Research, Stockholm, Sweden.
J
J
Chandra
S N
SN
Yang
M
M
Köhler
S
S
Zaitsev
L
L
Juntti-Berggren
P O
PO
Berggren
B
B
Zhivotovsky
S
S
Orrenius
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001323:Autoantibodies / Q000494:pharmacology*; D015220:Calcium Channels / Q000502:physiology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood* / Q000276:immunology; D006098:Granulocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D051381:Rats
D001323:Autoantibodies; D015220:Calcium Channels