[Effect of liposome-C-erbB2 antisense oligodeoxynucleotides on the growth and the chemotherapeutic drug sensitivity of human ovarian cancer transplanted in the omentum of nude mice].
OBJECTIVE
To investigate the effects of liposome-C-erbB2 antisense phosphorothioate oligodeoxynucleotides(S-ODNs) on the growth and the chemotherapeutic drug sensitivity of human ovarian cancer transplanted in the omentum of nude mice.


METHODS
Human ovarian cancer transplanted in the omentum of nude mice model was established, then divided into four groups: control group, experimental group, chemotherapeutic group, and experimental + chemotherapeutic group. Different treatments were given respectively. The weight of nude mice was observed and the morphology of tumor cells was observed by electromicroscope.


RESULTS
The growth inhibitory rate in the experimental group was 37%. There were more heterochromatins in the treated tumor cells. The growth inhibitory rate in the experimental + chemotherapeutic group was increased to 50%. There was no obvious alteration in the weight of experimental group.


CONCLUSIONS
The data in this study suggest that antisense therapy is an useful method of gene therapy in ovarian cancer; Moreover, it could enhance the effectiveness of antitumor drug.
Gynecology and Obstetrics Hospital, Shanghai Medical University, Shanghai 200011.
M
M
Shen
Y
Y
Feng
X
X
Zhou
D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use; D002945:Cisplatin / Q000627:therapeutic use; D005260:Female; D018734:Genes, erbB-2; D015316:Genetic Therapy; D006801:Humans; D008081:Liposomes; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*; D009852:Omentum; D010051:Ovarian Neoplasms / Q000188:drug therapy / Q000628:therapy*; D011897:Random Allocation; D014162:Transfection
D000970:Antineoplastic Agents; D008081:Liposomes; D020319:Oligodeoxyribonucleotides, Antisense; D002945:Cisplatin