Anti-tumor promoting activity of Asteracantha longifolia against experimental hepatocarcinogenesis in rats.
Vegetables, natural products of plant origin and numerous non-nutritive dietary constituents have been shown to play a salutary role in cancer chemoprevention. The present study aims to evaluate the chemopreventive efficacy of the methanol fraction of Asteracantha longifolia seed extract against development of 2-acetylaminofluorene (2-AAF)-selected gamma-glutamyl transpeptidase (gamma-GT)-positive foci following diethylnitrosamine (DEN) initiation. Treatment of rats with doses 200 and 400 mg/kg body weight of methanol extract of A. longifolia seeds on alternate days, subsequent to carcinogen treatment, for 6 weeks significantly reduced the incidence and size distribution of gamma-GT-positive foci and tumor formation. Administration of A. longifolia seeds significantly (P<0.001) ameliorated the activities of antioxidant enzymes, glutathione peroxidase (GPx) and catalase (CAT), in a dose-dependent manner. Prophylactic administration of seed extract simultaneous to 2-AAF in the diet, at same doses, significantly suppressed 2-AAF and partial hepatectomy (PH)-induced ornithine decarboxylase (ODC) activity and [(3)H]thymidine incorporation into hepatic DNA, in a dose-dependent manner. Assimilation of the quantitative foci data together with the findings of the modulation of tumor promoting markers give ample evidence to the anti-tumor promoting potential of A. longifolia seeds against chemically-induced hepatocarcinogenesis in Wistar rats.
Department of Medical Elementology & Toxicology, Faculty of Science, Jamia Hamdard (Hamdard University), 110 062, New Delhi, India. sahmed73@yahoo.com
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Ahmed
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Rahman
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Mathur
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Athar
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Sultana
D015073:2-Acetylaminofluorene / Q000008:administration & dosage; D000477:Alkylating Agents; D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002273:Carcinogens / Q000008:administration & dosage; D002374:Catalase / Q000378:metabolism; D004052:Diethylnitrosamine; D004305:Dose-Response Relationship, Drug; D005979:Glutathione Peroxidase / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000188:drug therapy / Q000517:prevention & control*; D008297:Male; D009955:Ornithine Decarboxylase / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds / Q000737:chemistry*; D013936:Thymidine; D013997:Time Factors; D005723:gamma-Glutamyltransferase / Q000187:drug effects / Q000378:metabolism
D000477:Alkylating Agents; D016588:Anticarcinogenic Agents; D002273:Carcinogens; D010936:Plant Extracts; D004052:Diethylnitrosamine; D015073:2-Acetylaminofluorene; D002374:Catalase; D005979:Glutathione Peroxidase; D005723:gamma-Glutamyltransferase; D009955:Ornithine Decarboxylase; D013936:Thymidine