Effects of gamma radiation on the allergenic and antigenic properties of milk proteins.
This study was carried out to evaluate the application of food irradiation technology as a method for reducing milk allergies. Bovine alpha-casein (ACA) and beta-lactoglobulin (BLG) were used as milk proteins. Using milk-hypersensitive patients' immunoglobulin E (IgE) and rabbit IgGs individually produced to ACA and BLG, the changes of allergenicity and antigenicity of irradiated proteins were observed by competitive indirect enzyme-linked immunosorbent assay. Allergenicity and antigenicity of the irradiated proteins were changed with different slopes of the inhibition curves. The disappearance of the band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and increase of the turbidity showed that solubility of the proteins decreased by radiation, and this decrease might be caused by agglomeration of the proteins. These results indicated that epitopes on milk allergens were structurally altered by gamma irradiation.
Lee
J W
JW
The Team for Radiation Food Science and Biotechnology, Korea Atomic Energy Research Institute, Yusong, Taejon.
Kim
J H
JH
Yook
H S
HS
Kang
K O
KO
Lee
S Y
SY
Hwang
H J
HJ
Byun
M W
MW
D000485:Allergens / Q000737:chemistry / Q000276:immunology / Q000528:radiation effects*; D000818:Animals; D002364:Caseins / Q000276:immunology / Q000528:radiation effects; D002417:Cattle; D004307:Dose-Response Relationship, Radiation; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005514:Food Irradiation; D005720:Gamma Rays; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology / Q000378:metabolism / Q000528:radiation effects; D007074:Immunoglobulin G / Q000378:metabolism / Q000528:radiation effects; D007782:Lactoglobulins / Q000276:immunology / Q000528:radiation effects; D016269:Milk Hypersensitivity / Q000517:prevention & control*; D008894:Milk Proteins / Q000276:immunology / Q000378:metabolism / Q000528:radiation effects*; D011485:Protein Binding / Q000528:radiation effects; D011817:Rabbits
D000485:Allergens; D002364:Caseins; D007074:Immunoglobulin G; D007782:Lactoglobulins; D008894:Milk Proteins; D007073:Immunoglobulin E