GcpE is involved in the 2-C-methyl-D-erythritol 4-phosphate pathway of isoprenoid biosynthesis in Escherichia coli.
In a variety of organisms, including plants and several eubacteria, isoprenoids are synthesized by the mevalonate-independent 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway. Although different enzymes of this pathway have been described, the terminal biosynthetic steps of the MEP pathway have not been fully elucidated. In this work, we demonstrate that the gcpE gene of Escherichia coli is involved in this pathway. E. coli cells were genetically engineered to utilize exogenously provided mevalonate for isoprenoid biosynthesis by the mevalonate pathway. These cells were then deleted for the essential gcpE gene and were viable only if the medium was supplemented with mevalonate or the cells were complemented with an episomal copy of gcpE.
Institute of Biochemistry, Academic Hospital Centre, Justus Liebig University, D-35392 Giessen, Germany. Boran.Altincicek@biochemie.med.uni-giessen.de
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Kollas
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Sanderbrand
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Wiesner
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Hintz
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D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D004798:Enzymes; D004896:Erythritol / Q000031:analogs & derivatives / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D017353:Gene Deletion; D005816:Genetic Complementation Test; D045782:Hemiterpenes; D008798:Mevalonic Acid / Q000378:metabolism; D008969:Molecular Sequence Data; D009943:Organophosphorus Compounds / Q000378:metabolism*; D013403:Sugar Phosphates / Q000378:metabolism*
C114232:2-C-methylerythritol 4-phosphate; D001426:Bacterial Proteins; D004798:Enzymes; D045782:Hemiterpenes; D009943:Organophosphorus Compounds; D013403:Sugar Phosphates; C421242:hydroxymethylbutenyl 4-diphosphate synthase; C004809:isopentenyl pyrophosphate; C043060:3,3-dimethylallyl pyrophosphate; D004896:Erythritol; D008798:Mevalonic Acid