[Clinico-diagnostic evaluation of changes in ATPase activity, lipid peroxidation and stability of red cell membranes in patients suffering from hemorrhagic fever with renal syndrome].
OBJECTIVE
To examine morphofunctional and metabolic features of erythrocytes affecting blood rheology in patients with hemorrhagic fever with renal syndrome (HFRS) for assessment of the disease severity.


METHODS
130 HFRS patients were examined using clinical, laboratory, serological tests and fluorescent antibody test. Activity of transport ATPase and content of lipid peroxidation (LPO) products in erythrocyte membranes were measured. These membranes stability was assessed by osmotic and acid resistance in different disease periods.


RESULTS
Inhibition of Na+, K+, Ca+ active ATPase of erythrocyte membrane occurred in all the examinees, LPO products rose. The membrane stability was more disturbed in moderate and severe HFRS, especially in polyuretic period.


CONCLUSIONS
Depression of ATPase activity, growth of LPO content in erythrocytes, their relationships can be used as indicators of red cell metabolic disorders, abnormal blood rheology, and eventually, in the disease prognosis. Early membrane defects detected by osmotic and acid resistance can improve the disease diagnosis and provide data on the condition's severity.
Ibragimova
L A
LA
Fazlyeva
R M
RM
D000251:Adenosine Triphosphatases / Q000097:blood*; D000328:Adult; D015415:Biomarkers / Q000097:blood; D001809:Blood Viscosity; D004910:Erythrocyte Membrane / Q000378:metabolism*; D005260:Female; D006480:Hemorrhagic Fever with Renal Syndrome / Q000097:blood* / Q000175:diagnosis; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D012720:Severity of Illness Index
D015415:Biomarkers; D000251:Adenosine Triphosphatases