Characterization of outer membrane proteins in Chlamydia trachomatis LGV serovar L2.
We used a photoactivatable, lipophilic reagent, 3'-(trifluoromethyl)-3-(m-[125I]iodophenyl)diazirine, to label proteins in the outer membrane of elementary bodies of Chlamydia trachomatis LGV serovar L2 and mass spectrometry to identify the labeled proteins. The identified proteins were polymorphic outer membrane proteins E, G, and H, which were made late in the developmental cycle, the major outer membrane protein, and a mixture of 46-kDa proteins consisting of the open reading frame 623 protein and possibly a modified form of the major outer membrane protein.
Department of Molecular Sciences, University of Tennessee Center for Health Sciences, Memphis, Tennessee 38163, USA.
R J
RJ
Tanzer
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TP
Hatch
D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry*; D002692:Chlamydia trachomatis / Q000737:chemistry* / Q000145:classification / Q000254:growth & development / Q000378:metabolism; D003978:Diazomethane / Q000031:analogs & derivatives / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D007457:Iodine Radioisotopes / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods; D018272:Porins
D001425:Bacterial Outer Membrane Proteins; D007457:Iodine Radioisotopes; D018272:Porins; C071677:omp1 protein, Chlamydia trachomatis; D003978:Diazomethane