Ontogenesis of protein kinase C betaII and its anchoring protein RACK1 in the maturation of alveolar macrophage functional responses.
Alveolar macrophages are the resident airway cells primarily responsible for the protection of the lungs against inhaled toxins and other biologically active materials. The purpose of this study was to investigate the maturation with age of alveolar macrophage functional responses. We characterised the ontogenesis of PKC betaII and its anchoring protein RACK1 in correlation with PKC-dependent immune functions, such as TNF-alpha, hydrogen peroxide production and lysozyme release in resident alveolar macrophages obtained from rats 2, 4 and 12 weeks old. Our results show an age-associated increase in the expression of PKC betaII and RACK1, which correlated with a maturation of alveolar macrophage functional responses.
Corsini
E
E
Laboratory of Toxicology, Institute of Pharmacological Sciences, University of Milan, Via Balzaretti 9, 20133, Milan, Italy. emanuela.corsini@unimi.it
Viviani
B
B
Lucchi
L
L
Marinovich
M
M
Racchi
M
M
Galli
C L
CL
D000375:Aging / Q000276:immunology*; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D006861:Hydrogen Peroxide / Q000378:metabolism; D007371:Interferon-gamma / Q000494:pharmacology; D007527:Isoenzymes / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology; D016676:Macrophages, Alveolar / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D008297:Male; D010455:Peptides / Q000378:metabolism*; D011493:Protein Kinase C / Q000096:biosynthesis*; D064546:Protein Kinase C beta; D051381:Rats; D017207:Rats, Sprague-Dawley; D000075370:Receptors for Activated C Kinase; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D007527:Isoenzymes; D008070:Lipopolysaccharides; D010455:Peptides; D000075370:Receptors for Activated C Kinase; D014409:Tumor Necrosis Factor-alpha; C070301:peptide I; D007371:Interferon-gamma; D006861:Hydrogen Peroxide; D011493:Protein Kinase C; D064546:Protein Kinase C beta; D013755:Tetradecanoylphorbol Acetate