Lack of tyrosine protein kinase regulation of L-type Ca2+ channel current in transfected cells stably expressing alpha1C-b Subunit.
Tyrosine protein kinase (Tyr-PK) regulation of L-type Ca2+ channel (CaL) current was studied in COS-7 cells expressing vascular smooth muscle-type alpha1C-b with no auxiliary subunit by using a whole-cell voltage clamp. The averaged peak amplitude of CaL currents was -0.33 +/- 0.03 at holding potential of -60 mV. Na(3)VO(4), genistein and phosphorylated p60(c-src) peptide had no effect on the current. Thus the alpha1C-b subunit may not be involved in Tyr-PK regulation of CaL current.
Department of Molecular and Cellular Physiology, College of Medicine, University of Cincinnati, Cincinnati, Ohio 45267-0576, USA. sunagam@med.u-ryukyu.ac.jp.
M
M
Sunagawa
M
M
Nakamura
T
T
Kosugi
F
F
Hofmann
N
N
Sperelakis
D000818:Animals; D020746:Calcium Channels, L-Type / Q000502:physiology*; D018929:Cell Culture Techniques; D002522:Chlorocebus aethiops; D004594:Electrophysiology; D007668:Kidney / Q000166:cytology; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000502:physiology; D018408:Patch-Clamp Techniques; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D014162:Transfection
D020746:Calcium Channels, L-Type; D011505:Protein-Tyrosine Kinases