B-Cell epitope mapping of the VapA protein of Rhodococcus equi: implications for early detection of R. equi disease in foals.
Linear B-cell epitopes of the Rhodococcus equi virulence-associated protein (VapA) were mapped using a synthetic peptide bank in this study. The peptides were screened in an enzyme-linked immunosorbent assay (ELISA) with a total of 70 sera from foals with current R. equi disease (51 sera), as well as from foals that had either recovered from R. equi infection 10 months previously (3 sera) or that had no known history of R. equi disease (16 sera). An epitope with the sequence NLQKDEPNGRA was identified and was universally recognized by all 51 sera from foals with R. equi disease and was not recognized by any of the other sera. There was poor reactivity between all sera and peptides relating to other areas of the VapA protein. It is proposed that an ELISA based upon a defined peptide epitope may be used in an improved serological diagnostic test for R. equi infection in foals.
School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, Australia.
T
T
Vanniasinkam
M D
MD
Barton
M W
MW
Heuzenroeder
D000193:Actinomycetales Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping; D018985:Epitopes, B-Lymphocyte; D006734:Horse Diseases / Q000175:diagnosis* / Q000382:microbiology; D006736:Horses; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology*; D009842:Oligopeptides / Q000276:immunology; D016929:Rhodococcus equi / Q000276:immunology* / Q000472:pathogenicity; D014774:Virulence; D037521:Virulence Factors
D001426:Bacterial Proteins; D018985:Epitopes, B-Lymphocyte; D008562:Membrane Glycoproteins; D009842:Oligopeptides; D037521:Virulence Factors