Crystallization of Clonorchis sinensis 26 kDa glutathione S-transferase and its fusion proteins with peptides of different lengths.
A Clonorchis sinensis 26 kDa glutathione S-transferase (CsGST) and its fusion proteins containing 14 and 48 amino-acid peptides at the N-terminus have been crystallized using polyethylene glycol monomethylether 550 as a precipitant. Crystals of the three proteins show very similar crystal properties: they diffract to at least 2.3 A resolution and belong to the orthorhombic space group P2(1)2(1)2(1). The unit-cell parameters of CsGST crystals were a = 66.64 (1), b = 68.91 (1), c = 123.41 (2) A, which are very close to those of the crystals of the two fusion proteins. In addition, CsGST fusion proteins containing varying extents of N-terminal-extended peptides are incorporated into a crystal, indicating that the extended peptides have little effect on crystal packing. These results suggest that the crystallization system of CsGST/peptide fusion protein may be generally applicable to obtain crystals of small peptides.
Han
Y H
YH
School of Biological Sciences, Chungbuk National University, Cheongju 360-763, South Korea.
Chung
Y H
YH
Kim
T Y
TY
Hong
S J
SJ
Choi
J D
JD
Chung
Y J
YJ
D000595:Amino Acid Sequence; D000818:Animals; D003004:Clonorchis sinensis / Q000201:enzymology; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D005982:Glutathione Transferase / Q000737:chemistry*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry*; D011993:Recombinant Fusion Proteins / Q000737:chemistry*
D010455:Peptides; D011993:Recombinant Fusion Proteins; D005982:Glutathione Transferase