The crystals of a mannose-specific jacalin-related lectin from Morus nigra are merohedrally twinned.
MornigaM, a lectin from Morus nigra, belongs to the mannose-binding subgroup of the family of jacalin-related plant lectins. It was crystallized in the P6(5) space group, with unit-cell parameters a = b = 110.74, c = 159.28 A. The partially merohedrally twinned crystals could be detwinned and a subsequent molecular-replacement solution could be found using the coordinates of jacalin. Preliminary analysis clearly shows the tetrameric assembly of this protein. Furthermore, data from MornigaM crystals soaked in a mannose solution were collected.
Laboratorium voor Analytische Chemie en Medicinale Fysicochemie, Faculteit Farmaceutische Wetenschappen, K. U. Leuven, Van Evenstraat 4, B-3000 Leuven, Belgium.
A
A
Rabijns
C
C
Verboven
H
H
Novoa de Armas
E J
EJ
Van Damme
W J
WJ
Peumans
C J
CJ
De Ranter
D003460:Crystallization; D007369:Interferon Inducers / Q000737:chemistry; D037102:Lectins / Q000737:chemistry* / Q000378:metabolism; D019684:Magnoliopsida / Q000737:chemistry*; D008358:Mannose / Q000378:metabolism*; D037121:Plant Lectins; D020836:Protein Structure, Quaternary; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D014961:X-Ray Diffraction
D007369:Interferon Inducers; D037102:Lectins; D037121:Plant Lectins; C043696:jacalin; D008358:Mannose