Cloning and DNA sequencing of the surface protein antigen I/II (PAa) of Streptococcus cricetus.
We have cloned and sequenced the gene encoding the surface protein antigen PAa (antigen I/II family) from Streptococcus cricetus E49 (serotype a) using degenerate PCR. The deduced amino acid sequence of PAa reveals two repeating regions (A region; alanine-rich region, P region; proline-rich region). Two additional tandem repeats were found in the A region and part of the P region was deleted compared to antigen I/II. Homology and phylogenetic analyses reveal that PAa is homologous to Streptococcus sobrinus PAg rather than Streptococcus mutans PAc. Using degenerate PCR a gene homologous to PAc was identified in Streptococcus intermedius, but not found in Streptococcus rattus or Streptococcus anginosus.
Department of Pharmacology, School of Dentistry, Iwate Medical University, 020-8505, Morioka, Japan.
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Tamura
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Kikuchi
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Shirato
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Kato
D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics; D000954:Antigens, Surface / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D006224:Cricetinae; D017931:DNA Primers; D005798:Genes, Bacterial; D006801:Humans; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D051381:Rats; D013291:Streptococcus / Q000145:classification / Q000235:genetics* / Q000276:immunology
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001426:Bacterial Proteins; D017931:DNA Primers; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; C055764:S-layer proteins