Oligonucleotide arrays for high resolution HLA typing.
The methodology and applications of oligonucleotide array technology are reviewed. An oligonucleotide array system for typing HLA-B alleles is described. Oligonucleotide probes representing all known polymorphisms in exons 2 and 3 of HLA-B are immobilized on glass slides. Exons 2 and 3 are amplified from human genomic DNA using fluorescent-labeled primers and subsequently allowed to hybridize to an oligonucleotide array on glass supports. The presence of positive hybridization is detected by fluorescence scanning. HLA-B alleles are assigned by quantitative analysis of hybridization patterns. Proof of principle has been established in blinded testing of heterozygous DNA samples.
Department of Molecular Biotechnology, University of Washington, Seattle, USA.
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Guo
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Hood
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Petersdorf
D006680:HLA Antigens / Q000235:genetics*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis
D006680:HLA Antigens