Persistent expression of a newly characterized Hyposoter didymator polydnavirus gene in long-term infected lepidopteran cell lines.
An Hyposoter didymator ichnovirus (HdIV) gene was stably maintained and efficiently transcribed in lepidopteran cell lines more than 3 years after HdIV infection. This K-gene had two introns and the fully spliced cDNA, named K19, comprised a short open reading frame and a long 3'-untranslated region with 13 imperfectly repeated sequences (44 to 102 nt). Transcripts related to the K-gene were detected in several long-term infected cell lines (Sf9, Spodoptera littoralis haemocytes, Trichoplusia ni). Conversely, no transcripts related to seven other viral cDNAs were detected, suggesting that the K-related DNA is selectively retained in long-term infected Sf9 cells. The function of the K-gene product and its association with stably transformed insect cell lines remains to be investigated.
Laboratoire de Recherches de Pathologie Comparée, INRA-CNRS, 30380 Saint-Christol-les-Alès, France1.
Anne-Nathalie
AN
Volkoff
Laboratoire de Recherches de Pathologie Comparée, INRA-CNRS, 30380 Saint-Christol-les-Alès, France1.
Janick
J
Rocher
Laboratoire de Recherches de Pathologie Comparée, INRA-CNRS, 30380 Saint-Christol-les-Alès, France1.
Pierre
P
Cérutti
Laboratoire de Recherches de Pathologie Comparée, INRA-CNRS, 30380 Saint-Christol-les-Alès, France1.
Marc C P
MCP
Ohresser
CNRS, Centre de Génétique Moléculaire, 91198 Gif-sur-Yvette, France2.
Yves
Y
d'Aubenton-Carafa
Laboratoire de Recherches de Pathologie Comparée, INRA-CNRS, 30380 Saint-Christol-les-Alès, France1.
Gérard
G
Devauchelle
Laboratoire de Recherches de Pathologie Comparée, INRA-CNRS, 30380 Saint-Christol-les-Alès, France1.
Martine
M
Duonor-Cérutti
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis; D005814:Genes, Viral; D007915:Lepidoptera / Q000821:virology*; D008969:Molecular Sequence Data; D018071:Polydnaviridae / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D004279:DNA, Viral