Dehydrogenases and transaminases in asymmetric synthesis.
Improved stereoselectivity in dehydrogenase-mediated reductions has been achieved by rationally designed gene overexpression and knockouts in Saccharomyces cerevisiae cells and by isolating and characterizing novel dehydrogenases from other organisms. Transaminases have been used to prepare unnatural amines and amino acids in good yields, particularly when the equilibria are shifted by selective product removal.
Stewart
J D
JD
Department of Chemistry, University of Florida, Gainesville 32611, USA. jds2@chem.ufl.edu
D000588:Amines / Q000138:chemical synthesis; D000596:Amino Acids / Q000138:chemical synthesis; D029721:Drosophila Proteins; D015870:Gene Expression / Q000235:genetics; D009124:Muscle Proteins / Q000235:genetics; D009930:Organic Chemicals / Q000138:chemical synthesis*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics*; D013237:Stereoisomerism; D013379:Substrate Specificity / Q000235:genetics / Q000502:physiology; D000637:Transaminases / Q000737:chemistry / Q000378:metabolism*
D000588:Amines; D000596:Amino Acids; D029721:Drosophila Proteins; D009124:Muscle Proteins; D009930:Organic Chemicals; C109229:ko protein, Drosophila; D010088:Oxidoreductases; D000637:Transaminases