Multi-beam polarized photometric detector for high-performance liquid chromatography.
A novel multi-beam polarized photometric detector (PPD) for high-performance liquid chromatography (HPLC) is described. By pairing a polarizing prism with a thin quartz plate as a retarder, many linear polarized beams are produced at every 1/2 wavelength of the plate, and the polarizing axes of the adjacent beams intersect each other. The addition of another prism inclining its polarizing axis by pi/4 against the first one enables the simultaneous measurement of optical rotations based on the PPD at many wavelengths. The combination of these optics with a photo-diode array detector can be used to construct a modulated type polarimeter. This detector is designed to measure the optical rotation of an analyte at its absorption band. The spline function connecting the points at 1/4 wavelengths of the plate was used as a baseline to extract the PPD waves. The use of the similarity factor as a noise filter gave high sensitivity. Application of the proposed technique to an analyte carrying the Cotton absorption band provided good results.
Toyama Institute of Health, Japan. lee07664@nifty.ne.jp
A
A
Yamamoto
S
S
Kodama
A
A
Matsunaga
K
K
Hayakawa
Y
Y
Yasui
M
M
Kitaoka
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013053:Spectrophotometry / Q000295:instrumentation*