Effect of PKC-zeta mediating Ang II-stimulated activation of CCDPK on rat cardiac fibroblast proliferation.
OBJECTIVE
To investigate whether the effect of angiotensin (Ang) II or epidermal growth factor (EGF) on cardiac fibroblast proliferation involved in activation of extracellular signal-regulated kinase (ERK) 1/2 or Ca(2+)-calmodulin dependent protein kinase(CCDPK) mediated by protein kinase C (PKC)-zeta.


METHODS
Relative activity of CCDPK was measured by Western blotting. DNA synthesis was assayed by [3H]thymidine incorporation.


RESULTS
PDBU caused no decrease in Ang II- and 10% FCS-stimulated CCDPK activity and DNA synthesis. In contrary, 65% or 75% EGF- or tetradecanoylphorbol acetate (TDPA, formally called PMA)--stimulated CCDPK activity and 38% or 42% [3H]thymidine incorporation treated by PDBU were inhibited, respectively. Meanwhile 70% and 72% CCDPK activities induced by Ang II and EGF were inhibited by PD 98059, respectively.


CONCLUSIONS
PKC-zeta mediated Ang II-induced activation of CCDPK and cardiac fibroblast proliferation.
Ding
B
B
Laboratory of Molecular Pharmacology, Hu-nan Medical University, Changsha 410078, China. dingboxj@public.cs.hn.cn
Huang
S L
SL
Zhang
S Q
SQ
Li
Y X
YX
D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology; D005419:Flavonoids / Q000494:pharmacology; D007527:Isoenzymes / Q000378:metabolism; D020929:Mitogen-Activated Protein Kinase Kinases / Q000037:antagonists & inhibitors; D009206:Myocardium / Q000166:cytology*; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D013755:Tetradecanoylphorbol Acetate; D000099221:Protein Kinase C zeta
D000804:Angiotensin II; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D004815:Epidermal Growth Factor; D005419:Flavonoids; D007527:Isoenzymes; D020929:Mitogen-Activated Protein Kinase Kinases; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; D000099221:Protein Kinase C zeta; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one