Complete sequences and phylogenetic analyses of a Singapore isolate of broad bean wilt fabavirus.
The complete nucleotide (nt) sequence of a Singapore isolate of broad bean wilt fabavirus from Megakepasma erythrochlamys L., designated BBWV-ME, was determined. Its bipartite genome consisted of two positive-sense single-stranded ribonucleic acids (RNA). RNA1 (5951 nt in length) encoded a putative protease cofactor, nucleotide triphosphate (NTP)-binding domain (helicase), viral genome-linked protein (VPg), protease and RNA-dependent RNA polymerase (RdRp). RNA2 (3607 nt in length) encoded a putative movement protein (MP) and coat proteins (CP). Genome organization of BBWV-ME was similar to other viruses in the Comoviridae family. Phylogenetic analyses showed that fabaviruses were more closely related to the comoviruses than the nepoviruses.
Koh
L H
LH
Department of Biological Sciences, The National University of Singapore.
Cooper
J I
JI
Wong
S M
SM
D001483:Base Sequence; D002213:Capsid / Q000235:genetics; D003001:Cloning, Molecular; D010450:Endopeptidases / Q000235:genetics; D007887:Fabaceae / Q000821:virology*; D019181:Fabavirus / Q000145:classification / Q000235:genetics*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D053830:Plant Viral Movement Proteins; D010946:Plants, Medicinal; D020365:RNA Helicases / Q000235:genetics; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012846:Singapore; D014764:Viral Proteins / Q000235:genetics
D053830:Plant Viral Movement Proteins; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase; D010450:Endopeptidases; D020365:RNA Helicases