Determination of protein-protein interactions of ICIn by the yeast two-hybrid system.
ICln is a ubiquitously expressed eukaryotic protein. Expression of the protein in Xenopus laevis oocytes, the knocking-down of the protein in fibroblasts, or the reconstitution of the protein in lipid bilayer led to the assumption that this protein is an ionic channel or a significant part thereof. However, other possible roles for ICln in potential regulatory mechanisms have been postulated, as diverse as regulator of cell morphology by interacting with the Skb1 protein and/or interaction with core spliceosomal proteins. Here we show that ICln is able to interact with SnRNP core proteins SmD1, SmD2, SmD3, SmX5 and SmB/B'.
Department of Physiology, Institute for Medical Biology and Human Genetics, University of Innsbruck, Austria.
A
A
Schmarda
F
F
Fresser
M
M
Gschwentner
J
J
Fürst
M
M
Ritter
F
F
Lang
G
G
Baier
M
M
Paulmichl
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015723:Gene Library; D017930:Genes, Reporter / Q000235:genetics; D006801:Humans; D007473:Ion Channels; D008969:Molecular Sequence Data; D011485:Protein Binding; D011506:Proteins / Q000235:genetics* / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D017411:Ribonucleoproteins, Small Nuclear / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D020798:Two-Hybrid System Techniques
C410035:CLNS1A protein, human; D007473:Ion Channels; C108240:Lsm2 protein, mouse; D011506:Proteins; D011993:Recombinant Fusion Proteins; D017411:Ribonucleoproteins, Small Nuclear