Cellular distribution of platelet-derived growth factor, transforming growth factor-beta, basic fibroblast growth factor, and their receptors in normal bone marrow.
We performed an immunohistochemical study on 5 normal marrow samples and 3 fibrotic marrow samples to investigate the cellular distribution of various isoforms of platelet-derived growth factor (PDGF), transforming growth factor-beta (TGF-beta), basic fibroblast growth factor (bFGF), and their corresponding receptors. Immature hematopoietic precursors strongly expressed PDGF-B, all the TGF-beta isoforms, PDGFRbeta, TGFbetaRI and II, and FGFR1, 3, and 4. Megakaryocytes stained primarily for PDGF-B, TGF-beta2-3, and PDGFRbeta. Histiocytes displayed intense TGF-beta1, bFGF, and FGFR2 expression. Fibroblasts and endothelial cells carried receptors for all the aforementioned cytokines. The last 2 cell types also expressed the ligand cytokines to varied degrees.
Division of Hematology and Internal Medicine, Mayo Clinic and Mayo Foundation, 200 First Street SW, Rochester, MN 55905, USA.
S Y
SY
Yoon
A
A
Tefferi
C Y
CY
Li
D001854:Bone Marrow Cells / Q000737:chemistry*; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D006133:Growth Substances / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D055728:Primary Myelofibrosis / Q000378:metabolism / Q000473:pathology; D020033:Protein Isoforms / Q000378:metabolism; D017978:Receptors, Growth Factor / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000378:metabolism
D006133:Growth Substances; D010982:Platelet-Derived Growth Factor; D020033:Protein Isoforms; D017978:Receptors, Growth Factor; D016212:Transforming Growth Factor beta; D016222:Fibroblast Growth Factor 2