Upregulation of ciliary neurotrophic factor (CNTF) and CNTF receptor alpha in rat kidney with ischemia-reperfusion injury.
Ciliary neurotrophic factor (CNTF) is presumed to play a role as a survival factor in neuronal cells, but little is known about its role in the kidney. To investigate this, the expression of CNTF and CNTF receptor alpha (CNTFR alpha) was analyzed in the ischemic rat kidney. An ischemia/reperfusion (I/R) injury was induced by clamping both renal arteries for 45 min. Animals were killed at 1, 2, 3, 5, 7, 14, and 28 d after ischemia. The expression of CNTF and CNTFR alpha was monitored by reverse transcription-PCR, in situ hybridization, immunoblotting, immunohistochemistry, and electron microscopy. In sham-operated rat kidneys, CNTF expression was weak and limited to the descending thin limb of the loop of Henle. With I/R injury, CNTF mRNA and protein expressions were strikingly increased as compared with the sham-operated rat kidney, and the immunoreactivity of CNTF was mainly observed in the regenerating proximal tubules. The expression of CNTFR alpha mRNA was also increased after I/R injury, and its location and expression patterns were similar to the expression of CNTF. These findings suggest a possible role of CNTF as a growth factor during renal tubular repair processes after I/R injury and an autocrine or paracrine function of CNTF acting against CNTFR alpha.
Yang
Chul Woo
CW
Division of Nephrology, Department of Internal Medicine, Kangnam St. Mary's Hospital, The Catholic University of Korea, Seoul, Korea.
Lim
Sun Woo
SW
Department of Anatomy, The College of Medicine, The Catholic University of Korea, Seoul, Korea.
Han
Ki Whan
KW
Department of Anatomy, The College of Medicine, The Catholic University of Korea, Seoul, Korea.
Ahn
Hee Jong
HJ
Division of Nephrology, Department of Internal Medicine, Kangnam St. Mary's Hospital, The Catholic University of Korea, Seoul, Korea.
Park
Jung Hee
JH
Division of Nephrology, Department of Internal Medicine, Kangnam St. Mary's Hospital, The Catholic University of Korea, Seoul, Korea.
Kim
Young Hee
YH
Department of Anatomy, The College of Medicine, The Catholic University of Korea, Seoul, Korea.
Kirsh
Matthias
M
Institute of Anatomy, University of Freiburg, Freiburg, Germany.
Cha
Jung Ho
JH
Department of Anatomy, The College of Medicine, The Catholic University of Korea, Seoul, Korea.
Park
Joo Hyun
JH
Division of Nephrology, Department of Internal Medicine, Kangnam St. Mary's Hospital, The Catholic University of Korea, Seoul, Korea.
Kim
Yong Soo
YS
Division of Nephrology, Department of Internal Medicine, Kangnam St. Mary's Hospital, The Catholic University of Korea, Seoul, Korea.
Kim
Jin
J
Department of Anatomy, The College of Medicine, The Catholic University of Korea, Seoul, Korea.
Bang
Byung Kee
BK
Division of Nephrology, Department of Internal Medicine, Kangnam St. Mary's Hospital, The Catholic University of Korea, Seoul, Korea.
D000818:Animals; D002455:Cell Division; D020934:Ciliary Neurotrophic Factor / Q000235:genetics / Q000378:metabolism*; D015151:Immunoblotting; D007150:Immunohistochemistry; D007511:Ischemia / Q000378:metabolism* / Q000473:pathology; D007668:Kidney / Q000378:metabolism* / Q000473:pathology; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D020033:Protein Isoforms / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020801:Receptor, Ciliary Neurotrophic Factor / Q000235:genetics / Q000378:metabolism*; D012016:Reference Values; D012079:Renal Circulation; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology; D014018:Tissue Distribution; D015854:Up-Regulation
D020934:Ciliary Neurotrophic Factor; D018809:Proliferating Cell Nuclear Antigen; D020033:Protein Isoforms; D012333:RNA, Messenger; D020801:Receptor, Ciliary Neurotrophic Factor