Infection of human lung fibroblasts with Epstein-Barr virus causes increased IL-1beta and bFGF production.
An association between Epstein-Barr virus (EBV) infection and fibroblast proliferation in the interstitial spaces of the lung has been suggested in idiopathic interstitial pneumonia. In this study we show that EBV can infect human lung fibroblasts in vitro. A primary-cultured human lung fibroblast cell line, designated CCD-32Lu, expressed EBV nuclear antigen 1 after coculture with lethally irradiated EBV producing cells. The infection further induced CCD-32Lu cells to produce the fibrogenic cytokines basic fibroblast growth factor (bFGF) and interleukin-1beta. These findings indicate that lung fibroblasts may be a target for EBV infection and suggest that EBV may play a role in increased production of these cytokines and induce fibroblast proliferation in idiopathic interstitial pneumonia.
Adachi
H
H
5th Department of Internal Medicine, Tokyo Medical University, Ibaraki, Japan. ha0723@sb3:so-net.ne.jp
Saito
I
I
Horiuchi
M
M
Ishii
J
J
Nagata
Y
Y
Mizuno
F
F
Nakamura
H
H
Yagyu
H
H
Takahashi
K
K
Matsuoka
T
T
D019138:Antibodies, Blocking / Q000494:pharmacology; D000914:Antibodies, Viral / Q000276:immunology; D015153:Blotting, Western; D002455:Cell Division; D002460:Cell Line; D018920:Coculture Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D020031:Epstein-Barr Virus Infections / Q000378:metabolism*; D019309:Epstein-Barr Virus Nuclear Antigens / Q000032:analysis / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis*; D005347:Fibroblasts / Q000821:virology*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D004854:Herpesvirus 4, Human / Q000502:physiology*; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis*; D008168:Lung / Q000166:cytology; D017563:Lung Diseases, Interstitial / Q000209:etiology / Q000378:metabolism / Q000821:virology; D009500:Neutralization Tests; D016212:Transforming Growth Factor beta / Q000096:biosynthesis; D053773:Transforming Growth Factor beta1
D019138:Antibodies, Blocking; D000914:Antibodies, Viral; D019309:Epstein-Barr Virus Nuclear Antigens; D007375:Interleukin-1; C508960:TGFB1 protein, human; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D016222:Fibroblast Growth Factor 2