[TNF-alpha production and antitumor effect of rhIL-2-activated bone marrow cells].
OBJECTIVE
To explore the mechanism of the cytotoxic effects of bone marrow cells activated with recombinant human interleukin-2(rhIL-2).


METHODS
The cytotoxicity of rhIL-2-activated bone marrow (ABM) and the TNF-alpha level in the supernant of ABM culture were measured after activated for 24 and 48 hours.


RESULTS
The cytotoxic activity against HL-60 cells and the level of TNF-alpha were significantly increased in ABM as compared with that in non-ABM(P < 0.01). Meanwhile, there was a significantly positive relationship between the TNF-alpha levels in supernant and cytotoxicities of ABM.


CONCLUSIONS
TNF-alpha produced by rhIL-2 activated bone marrow cells involved in the antitumor effect of ABM.
Zhu
B
B
Xijing Hospital, Fourth Military Medical University, Xian 710032.
Yang
P
P
Sun
B
B
D001854:Bone Marrow Cells / Q000166:cytology*; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D018922:HL-60 Cells / Q000378:metabolism; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D011994:Recombinant Proteins / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D007376:Interleukin-2; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha