Recombinant vaccines against infectious hematopoietic necrosis virus: production by the Caulobacter crescentus S-layer protein secretion system and evaluation in laboratory trials.
We report the development of an IHNV vaccine produced by a new protein production system based on the bacterium Caulobacter crescentus. The subunit vaccines that were tested contain a 184 amino acid segment of the IHNV glycoprotein in different fusion arrangements with the C. crescentus S-layer protein. Relative percent survival of 26 to 34% was demonstrated in rainbow trout fry for a vaccine that contained the 184 amino acid segment of the IHNV glycoprotein fused to the C-terminal one-quarter of the S-layer protein. Inclusion of the universal mammalian T-cell epitopes developed from the measles fusion protein or the tetanus toxin protein did not increase the effectiveness of the IHNV-G/S-layer recombinant protein.
Department of Microbiology and the Center for Salmon Disease Research, Oregon State University, Corvallis 97331-3804, USA.
B
B
Simon
J
J
Nomellini
P
P
Chiou
W
W
Bingle
J
J
Thornton
J
J
Smit
J A
JA
Leong
D000276:Adjuvants, Immunologic / Q000627:therapeutic use; D000818:Animals; D017756:Aquaculture; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001426:Bacterial Proteins; D016935:Caulobacter crescentus / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D005393:Fish Diseases / Q000517:prevention & control*; D008562:Membrane Glycoproteins; D017686:Oncorhynchus mykiss; D011993:Recombinant Fusion Proteins / Q000276:immunology; D012209:Rhabdoviridae / Q000276:immunology*; D018353:Rhabdoviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D014759:Viral Envelope Proteins / Q000276:immunology*; D014765:Viral Vaccines / Q000096:biosynthesis* / Q000276:immunology
D000276:Adjuvants, Immunologic; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D018984:Epitopes, T-Lymphocyte; C104040:IHNVG protein, Infectious hematopoietic necrosis virus; D008562:Membrane Glycoproteins; D011993:Recombinant Fusion Proteins; C055764:S-layer proteins; D014759:Viral Envelope Proteins; D014765:Viral Vaccines