Efficiency of recombination reactions catalyzed by class 1 integron integrase IntI1.
The class 1 integron integrase, IntI1, recognizes two distinct types of recombination sites, attI sites, found in integrons, and members of the 59-be family, found in gene cassettes. The efficiencies of the integrative version of the three possible reactions, i.e., between two 59-be, between attI1 and a 59-be, or between two attI1 sites, were compared. Recombination events involving two attI1 sites were significantly less efficient than the reactions in which a 59-be participated, and the attI1 x 59-be reaction was generally preferred over the 59-be x 59-be reaction. Recombination of attI1 with secondary sites was less efficient than the 59-be x secondary site reaction.
CSIRO Molecular Science, Sydney Laboratory, North Ryde, New South Wales 1670, Australia.
C M
CM
Collis
G D
GD
Recchia
M J
MJ
Kim
H W
HW
Stokes
R M
RM
Hall
D001287:Attachment Sites, Microbiological / Q000235:genetics; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D019426:Integrases / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D011995:Recombination, Genetic
D019426:Integrases; C115006:integron integrase IntI1