Depth of immersion of fluorescent chromophores in biomembranes studied by quenching with nitroxide radical.
A novel method has been developed for measuring depth of immersion of a fluorescent chromophore in biological matrices, such as biomembranes. The method is based on dynamic quenching of chromophore fluorescence by a nitroxide probe freely diffused in solution. Theoretical considerations and experimental evidences relating to the method are discussed. The proposed method was applied to investigation of lecithin liposomes and membranes from Bacillus subtilis modified by the photochrome-fluorescent probe 4,4'-dimethylaminocyanostilbene.
Department of Chemistry, Ben-Gurion University of Negev, P.O. Box 84105, Beer-Sheva, Israel.
N V
NV
Strashnikova
N
N
Medvedeva
G I
GI
Likhtenshtein
D001412:Bacillus subtilis / Q000166:cytology / Q000378:metabolism; D001703:Biophysics / Q000379:methods*; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D003497:Cyclic N-Oxides / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D016166:Free Radical Scavengers / Q000737:chemistry; D008081:Liposomes; D008962:Models, Theoretical; D009570:Nitriles / Q000737:chemistry*; D009589:Nitrogen Oxides / Q000737:chemistry*; D012996:Solutions; D013113:Spin Labels; D013267:Stilbenes / Q000737:chemistry*
C431544:4,4'-dimethylaminocyanostilbene; D003497:Cyclic N-Oxides; D005456:Fluorescent Dyes; D016166:Free Radical Scavengers; D008081:Liposomes; D009570:Nitriles; D009589:Nitrogen Oxides; D012996:Solutions; D013113:Spin Labels; D013267:Stilbenes; C039900:nitroxyl; C001803:tempol