Effect of different drugs on the level of DNA-hydrolyzing polyclonal IgG antibodies in sera of patients with Hashimoto's thyroiditis and nontoxic nodal goiter.
BACKGROUND
DNA-hydrolyzing IgG antibodies have been detected recently in the sera of patients with several autoimmune diseases.


METHODS
The relative activity of DNA-hydrolyzing IgG from the sera of patients with Hashimoto's thyroiditis and with non-toxic nodal goiter as a function of the patient's condition were measured. The effect of different drugs on the level of DNA-hydrolyzing IgG, functional activity of thyroid gland, and improvement of clinical condition of two groups of patients were analyzed.


RESULTS
We demonstrate here for the first time that IgG from peripheral blood of patients with Hashimoto's thyroiditis (65%) and non-toxic nodal goiter (38%) possesses DNAse activity. The relative level-specific activity of IgGs in hydrolysis of DNA increases with the enhancement of the relative amount of antibodies against thyroglobulin and all patients with hypothyroidism (namely a reduced concentration of thyroxine and triiodothyronine and enhanced level of thyrotropic hormone) are characterized by a high level of catalytic antibodies.


CONCLUSIONS
The very widely used therapy of patients with thyroxine led only to a temporary change of the hormone concentration in the blood but did not affect the level of DNA-hydrolyzing antibodies. However, treatment with an immunosuppressive drug plaquenil (7-chloro-4(beta-diethylamine-alpha-methylbutylamie)quinoline), significantly decreased the DNA-hydrolyzing activity of Abs, which correlated with enhancement of thyroid hormone concentrations, elevation of functional activity of the thyroid gland, and improvement of the clinical state of the patients.
Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of Russian Academy of Sciences, Novosibirsk, Russia. nevinsky@niboch.nsc.ru
G A
GA
Nevinsky
A A
AA
Breusov
A G
AG
Baranovskii
A V
AV
Prints
T G
TG
Kanyshkova
A V
AV
Galvita
V A
VA
Naumov
V N
VN
Buneva
D000974:Antibodies, Antinuclear / Q000097:blood* / Q000302:isolation & purification; D002850:Chromatography, Gel; D004247:DNA / Q000097:blood*; D004587:Electrophoresis, Agar Gel; D006044:Goiter, Nodular / Q000097:blood* / Q000188:drug therapy / Q000276:immunology; D006801:Humans; D006868:Hydrolysis; D006886:Hydroxychloroquine / Q000627:therapeutic use*; D007074:Immunoglobulin G / Q000276:immunology*; D013967:Thyroiditis, Autoimmune / Q000097:blood* / Q000188:drug therapy / Q000276:immunology; D013974:Thyroxine / Q000627:therapeutic use*
D000974:Antibodies, Antinuclear; D007074:Immunoglobulin G; D006886:Hydroxychloroquine; D004247:DNA; D013974:Thyroxine