Identification of hTLR10: a novel human Toll-like receptor preferentially expressed in immune cells.
Herein we describe the isolation of a cDNA encoding a novel human Toll-like receptor (hTLR) that we term hTLR10. Human TLR10 contains 811 amino acid residues. Deduced amino acid sequence analysis reveals that like the other known hTLRs (hTLR1-9) it is characterized by a signal peptide followed by multiple leucine-rich repeats (LRRs), a cysteine-rich domain, a transmembrane sequence and a cytoplasmic domain homologous to that of the human interleukin-1 receptor. Phylogenetic analysis indicates that among all the hTLRs, hTLR10 is most closely related to hTLR1 and hTLR6; the overall amino acid identity is 50% and 49%, respectively. hTLR10 mRNA is most highly expressed in lymphoid tissues such as spleen, lymph node, thymus, and tonsil. Expression analysis of cell lines indicates a predominance in a variety of immune cell types. Thus, hTLR10 is preferentially expressed in tissues and cells involved in immune responses.
Chuang
T
T
Department of Immunology, The Scripps Research Institute, 10550 North Torrey Pines Road, 92037, La Jolla, CA, USA.
Ulevitch
R J
RJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D018076:DNA, Complementary; D015870:Gene Expression; D006801:Humans; D008198:Lymph Nodes / Q000378:metabolism; D008969:Molecular Sequence Data; D014066:Palatine Tonsil / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013154:Spleen / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism; D051201:Toll-Like Receptor 10
D018076:DNA, Complementary; D011956:Receptors, Cell Surface; C475955:TLR10 protein, human; D051201:Toll-Like Receptor 10