A calcium-binding protein with four EF-hand motifs in Streptomyces ambofaciens.
A gene (cabA) encoding a calcium-binding protein was cloned from Streptomyces ambofaciens. CabA was 180 amino acid residues long and contained four typical EF-hand motifs bearing high sequence similarity to the calcium-binding sites in calmodulin. Consistent with this, CabA showed distinct calcium-binding activity, comparable to bovine brain calmodulin. cabA was transcribed throughout growth, as found by S1 nuclease mapping. Southern hybridization experiments showed that a single copy of cabA was present in various Streptomyces species. A hypothetical relationship between CabA and aerial mycelium formation in this strain was examined, since S. ambofaciens showed calcium-dependent aerial mycelium formation. However, disruption of cabA or overexpression of cabA in S. ambofaciens caused no detectable phenotypic changes.
Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Japan.
T
T
Yonekawa
Y
Y
Ohnishi
S
S
Horinouchi
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins; D015139:Blotting, Southern; D002118:Calcium / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D002147:Calmodulin / Q000235:genetics / Q000378:metabolism; D002417:Cattle; D020832:EF Hand Motifs / Q000235:genetics; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism; D013302:Streptomyces / Q000737:chemistry* / Q000235:genetics
D001426:Bacterial Proteins; C432589:CabA protein, Streptomyces ambofaciens; D002135:Calcium-Binding Proteins; D002147:Calmodulin; D015719:Single-Strand Specific DNA and RNA Endonucleases; D002118:Calcium