Inhibition of cutaneous UV light-induced tumor necrosis factor-alpha protein production by Allotrap 1258, a novel immunomodulatory peptide.
Peptides derived from the heavy chain of the HLA Class-I molecules have been shown to modulate immune responses both in vivo and in vitro. Using a computer-aided rational drug design approach, novel immunomodulatory peptides were designed based on peptide 2702.75-85, derived from HLA-B2702. Several peptides were identified which had increased immunomodulatory activity, including peptides RDP1258 and its D-isomer the peptide Allotrap 1258. The present study using Skh/hr hairless mouse skin model evaluated the in vivo effects of Allotrap 1258 on acute UVB-induced skin inflammation. Here we demonstrate that intraperitoneal administration of Allotrap 1258 1 h prior to UV exposure resulted in significantly diminished levels of UV-induced tumor necrosis factor (TNF)-alpha protein production in the epidermis but had no effect on other parameters of the acute UV-induced inflammatory response. By virtue of its ability to suppress TNF-alpha protein production, Allotrap 1258 could prove to be an effective modulator of inflammatory responses.
Oberyszyn
T M
TM
Department of Molecular Virology, Immunology & Medical Genetics, College of Medicine and Public Health, Ohio State University, Columbus, OH, USA.
Robertson
F M
FM
Tober
K L
KL
Ross
M S
MS
Parrett
M L
ML
Wilgus
T A
TA
Iyer
S
S
Woo
J
J
Buelow
R
R
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D005260:Female; D007150:Immunohistochemistry; D051379:Mice; D008812:Mice, Hairless; D010455:Peptides / Q000494:pharmacology; D012867:Skin / Q000276:immunology / Q000473:pathology / Q000528:radiation effects*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000235:genetics; D014466:Ultraviolet Rays / Q000009:adverse effects
D000276:Adjuvants, Immunologic; D010455:Peptides; D014409:Tumor Necrosis Factor-alpha; C092373:allotrap