Carboproteins: a 4-alpha-helix bundle protein model assembled on a D-galactopyranoside template.
We have recently introduced the concept of monosaccharides as templates for de novo design of protein models and described the synthesis of a model 'carbopeptide'. Here, we report the synthesis of a 64 amino acid (AA) 'carboprotein' by chemoselective ligation of a C-terminal hexadecapeptide aldehyde to a tetra-aminooxy functionalized methyl alpha-D-galactopyranoside (D-Galp) template. Biophysical characterizations by CD spectroscopy and NMR amide H-D exchange experiments indicated that the four-stranded carboprotein forms a 4-alpha-helix bundle structure.
Brask
J
J
Department of Organic Chemistry, Technical University of Denmark, Lyngby.
Jensen
K J
KJ
D002942:Circular Dichroism; D005690:Galactose / Q000737:chemistry*; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D011487:Protein Conformation; D013698:Templates, Genetic
D010455:Peptides; D005690:Galactose