Tissue-specific expression of two metallothionein genes in common carp during cadmium exposure and temperature shock.
Two metallothionein cDNA isoforms (MT-1 and MT-2) were isolated from carp (Cyprinus carpio) by RT-PCR. Sequence analysis of the cDNAs revealed two amino acid differences between the coding regions and markedly different 3'-untranslated ends. Gene-specific primers were selected and used in RT-PCR reactions to measure the basal MT-1 and MT-2 mRNA levels and to follow the inducer-specific expression of MT genes in different tissues during in vivo studies. In the brain and muscle, the uninduced levels of the two MT mRNAs were similar. In the kidney and liver, the MT-1 gene product predominated, while in the heart the relative expression levels of the two genes were opposite. Both the MT-1 and MT-2 mRNA levels increased with Cd concentration in a time- and dose-dependent manner. The expression of MT-2, however, was more responsive to a high Cd concentration. In parallel with the induction of the MTs by Cd, we followed the accumulation of this metal in the kidney and liver. Although the Cd level was always higher in the kidney during treatment, the rate of accumulation was higher in the liver. Cold stress resulted in a significantly higher induction of MT-1 than of MT-2, while heat shock had no effect on the expression of either gene.
University of Szeged, Faculty of Science, Department of Biochemistry, P.O. Box 533, H-6701, Szeged, Hungary. hermesz@biocom.bio.u-szeged.hu
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D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry / Q000187:drug effects; D002104:Cadmium / Q000494:pharmacology*; D002347:Carps / Q000235:genetics*; D003080:Cold Temperature; D005786:Gene Expression Regulation; D007527:Isoenzymes; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008668:Metallothionein / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D012333:RNA, Messenger; D012680:Sensitivity and Specificity; D012689:Sequence Homology, Nucleic Acid; D013997:Time Factors
D007527:Isoenzymes; D012333:RNA, Messenger; D002104:Cadmium; D008668:Metallothionein