[Experimental study on mice scheduled-bone marrow transplantation].
OBJECTIVE
To enhance the grafting efficiency of bone marrow transplantation.


METHODS
Lethally irradiated recipient Kunming mice were transplanted with bone marrow cells from normal Kunming mice either in one large (10(7)) number (BMT group) or in four separate small (10(5)-10(6)) numbers (scheduled BMT group) and the survival rate, hematopoiesis reconstitution and acute graft versus host disease (aGVHD) were compared between the two groups.


RESULTS
Both BMT and SBMT (10(5) x 4) groups obtained the same survival rate of 30%. In SBMT (10(6) x 4) group, the peripheral WBC count, bone marrow nucleated cells and CFU-E, CFU-GM, CFU-S and CFU-F yields all returned to normal 17 days after irradiation. The degree of GVHD in SBMT group was less severe than that in BMT group, and the survival rate (60%) was significantly higher than that in BMT group (30%).


CONCLUSIONS
SBMT can significantly enhance the grafting efficiency.
Institute of Radiation Medicine, Second Military Medical University, Shanghai 200433.
Y
Y
Xiang
R
R
Yang
X
X
Meng
D000818:Animals; D002452:Cell Count; D006087:Graft vs Host Reaction; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods; D006412:Hematopoietic Stem Cells / Q000473:pathology; D051379:Mice; D019172:Transplantation Conditioning; D014916:Whole-Body Irradiation