Stable carbon isotope fractionations of the hyperthermophilic crenarchaeon Metallosphaera sedula.
The stable carbon isotopic compositions of the inorganic carbon source, bulk cell material, and isoprenoid lipids of the hyperthermophilic crenarchaeon Metallosphaera sedula, which uses a 3-hydroxypropionate-like pathway for autotrophic carbon fixation, have been measured. Bulk cell material was approximately 3 per thousand enriched in 13C relative to the dissolved inorganic carbon, and 2 per thousand depleted in 13C relative to isoprenoid membrane lipids. The isotope data suggested that M. sedula uses mainly bicarbonate rather than CO(2) as inorganic carbon source, which is in accordance with a 3-hydroxypropionate-like carbon fixation pathway. To the best of our knowledge this is the first report of 13C fractionation effects of such a hyperthermophilic crenarchaeon.
Netherlands Institute for Sea Research (NIOZ), Department of Marine Biogeochemistry and Toxicology, Texel, The Netherlands. mmeer@nioz.nl
M T
MT
van der Meer
S
S
Schouten
W I
WI
Rijpstra
G
G
Fuchs
J S
JS
Sinninghe Damsté
D001639:Bicarbonates / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism*; D002247:Carbon Isotopes / Q000032:analysis*; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000031:analogs & derivatives* / Q000378:metabolism*; D008563:Membrane Lipids / Q000737:chemistry / Q000378:metabolism; D016943:Sulfolobaceae / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D013696:Temperature
D001639:Bicarbonates; D002247:Carbon Isotopes; D003470:Culture Media; D008563:Membrane Lipids; D002245:Carbon Dioxide; D019344:Lactic Acid; C031601:hydracrylic acid