Early infiltration of renal allografts with 27E10-positive macrophages and graft outcome.
Recently, we have demonstrated that acute cellular rejection is correlated with a massive infiltration of 27E10-positive macrophages. To examine the distribution of macrophage differentiation markers in the infiltrate in the very early post-transplantation period, two biopsies were taken intraoperatively, approximately 1 h following reperfusion, in each of 16 renal transplant recipients. One biopsy was taken for conventional histology and the other biopsy was snap-frozen. The sections were stained using an ABC indirect immunoperoxidase technique. A panel of monoclonal antibodies against three macrophage differentiation markers (27E10, 25F9 and RM3/1) was used to stain the sections. Using the early inflammation macrophage marker 27E10, there was an unexpected strong staining in 3 out of 16 biopsies. This severe infiltration of 27E10-positive macrophages with 10-20 macrophages per high power field (compared to 0-2 in others) was correlated in all cases with a poor outcome of the graft. All seven kidneys with no 27E10-positive infiltration showed a good function 6 weeks post-transplantation. The other macrophage markers, 25F9 and RM3/1, showed a less marked correlation with graft outcome. In conclusion, a massive infiltration of renal allografts with 27E10-positive macrophages 1 h post-transplantation may be a very early predictor of poor graft outcome.
Nephrology Division, Klinikum Grosshadern, University of Munich, Germany.
K
K
Burkhard
G O
GO
Hofmann
A
A
Bösnecker
G
G
Hillebrand
W D
WD
Illner
P
P
Petersen
W
W
Land
D000954:Antigens, Surface / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D001706:Biopsy; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007150:Immunohistochemistry; D007249:Inflammation; D016030:Kidney Transplantation / Q000276:immunology* / Q000473:pathology / Q000502:physiology; D039841:Leukocyte L1 Antigen Complex; D008264:Macrophages / Q000276:immunology* / Q000473:pathology; D008562:Membrane Glycoproteins / Q000032:analysis*; D019006:Neural Cell Adhesion Molecules / Q000032:analysis*; D016896:Treatment Outcome
D000954:Antigens, Surface; D015415:Biomarkers; D039841:Leukocyte L1 Antigen Complex; D008562:Membrane Glycoproteins; D019006:Neural Cell Adhesion Molecules