Electrostatic surface plasmon resonance: direct electric field-induced hybridization and denaturation in monolayer nucleic acid films and label-free discrimination of base mismatches.
We demonstrate that in situ optical surface plasmon resonance spectroscopy can be used to monitor hybridization kinetics for unlabeled DNA in tethered monolayer nucleic acid films on gold in the presence of an applied electrostatic field. The dc field can enhance or retard hybridization and can also denature surface-immobilized DNA duplexes. Discrimination between matched and mismatched hybrids is achieved by simple adjustment of the electrode potential. Although the electric field at the interface is extremely large, the tethered single-stranded DNA thiol probes remain bound and can be reused for subsequent hybridization reactions without loss of efficiency. Only capacitive charging currents are drawn; redox reactions are avoided by maintaining the gold electrode potential within the ideally polarizable region. Because of potential-induced changes in the shape of the surface plasmon resonance curve, we account for the full curve rather than simply the shift in the resonance minimum.
Heaton
R J
RJ
Department of Chemistry, Boston University, 590 Commonwealth Avenue, Boston, MA 02215, USA.
Peterson
A W
AW
Georgiadis
R M
RM
D020137:Base Pair Mismatch; D004277:DNA, Single-Stranded / Q000138:chemical synthesis / Q000737:chemistry; D004563:Electrochemistry; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000379:methods; D013873:Thionucleotides / Q000138:chemical synthesis / Q000737:chemistry
D004277:DNA, Single-Stranded; D009841:Oligonucleotides; D013873:Thionucleotides