[The morphological and functional changes of pulmonary intravascular macrophages induced by LPS].
OBJECTIVE
To study the roles of pulmonary intravascular macrophages (PIMs) on infective acute lung injury (ALI).


METHODS
Porcine pulmonary blood vessels were flushed by modified Morton's method, PIMs were isolated by adhesion method and identified with the features at both light and electron microscopic level. The activity of IL-1 beta, and content of IL-6, IL-8 and TNF alpha in the culture supernatants were measured by thymocyte proliferation or ELISA, respectively.


RESULTS
Enlarged and increased pseudopods, and increased amount of lysosomes and phagosomes were found in the PIM stimulated with lipopolysaccharide (LPS, 10 micrograms/ml); the releases of TNF alpha, IL-1 beta, IL-6 and IL-8 were increased significantly as compared with the level of pre-stimulation of LPS (P < 0.01), and reaching their peaks at 1 h, 2 h, 4 h and 6 h after LPS stimulation, respectively.


CONCLUSIONS
The isolation of porcine PIMs can be completed with modified Morton's method; the phagocytosis and secretion of PIMs are more active after LPS stimulation. In the pathogenesis of ALI, TNF alpha and IL-1 beta may play an important role at its early stage; however, the IL-6 and IL-8 may be associated with the pathophysiological changes at the later stage of ALI.
Institute of Respiratory Diseases, Xingqiao Hospital, Third Military Medical University, Chongqing 400037.
S
S
Li
Z
Z
Chen
J
J
Jin
D000818:Animals; D002478:Cells, Cultured; D007375:Interleukin-1 / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008168:Lung / Q000098:blood supply*; D016676:Macrophages, Alveolar / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure*; D013552:Swine; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D007375:Interleukin-1; D015850:Interleukin-6; D016209:Interleukin-8; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha