Secretory leukocyte protease inhibitor in ovarian endometriomas following GnRH agonist therapy.
OBJECTIVE
To determine whether expression of secretory leukocyte protease inhibitor is affected in tissue and peritoneal fluid of women with ovarian endometriomas treated with GnRH analogues.


METHODS
In 32 women with endometriomas (17 untreated and 15 treated with GnRH analogue) and 21 with ovarian cystadenomas, we examined the expression of secretory leukocyte protease inhibitor messenger RNA (mRNA) by Northern blot analysis; protein distribution was measured immunohistochemically. Concentrations of secretory leukocyte protease inhibitor in peritoneal fluid were measured by enzyme-linked immunosorbent assay. Expression of secretory leukocyte protease inhibitor in endometrioma explants in vitro was also studied with and without the GnRH analogue treatment.


RESULTS
Secretory leukocyte protease inhibitor mRNA expression was identified only in untreated endometriomas. In the GnRH agonist-treated endometriomas, the semiquantitative H-score for secretory leukocyte protease inhibitor immunostaining was significantly lower than that for untreated endometriomas (P <.001). The peritoneal fluid of the GnRH agonist-treated women also contained significantly lower concentrations of secretory leukocyte protease inhibitor (median 76 ng/mL, interquartile range 51-131 ng/mL; P <.001) than untreated women (124 ng/mL, 70-186 ng/mL). Secretory leukocyte protease inhibitor in endometrioma explants in vitro was significantly inhibited by the GnRH analogue (P <.05).


CONCLUSIONS
Expression of secretory leukocyte protease inhibitor in tissue and peritoneal fluid of women with ovarian endometriomas was decreased by GnRH agonist treatment.
Department of Obstetrics and Gynecology, Nagoya City University Medical School, Nagoya, Japan. suzumori@bcm.tmc.edu
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Suzumori
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Sato
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Ikuta
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Suzumori
D000328:Adult; D001202:Ascitic Fluid / Q000378:metabolism; D015152:Blotting, Northern; D016022:Case-Control Studies; D004715:Endometriosis / Q000188:drug therapy / Q000378:metabolism*; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000819:agonists* / Q000031:analogs & derivatives*; D006801:Humans; D007150:Immunohistochemistry; D016729:Leuprolide / Q000494:pharmacology* / Q000627:therapeutic use; D008875:Middle Aged; D010049:Ovarian Diseases / Q000188:drug therapy / Q000378:metabolism*; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins / Q000187:drug effects* / Q000235:genetics
D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins; D007987:Gonadotropin-Releasing Hormone; D016729:Leuprolide