Studies on the energy release of rice mitochondria under different conditions by means of microcalorimetry.
The thermodynamic and kinetic behaviors of energy release of mitochondria isolated from rice (Oryza sative L.) were studied by using a LKB 2277 Bioactivity Monitor under different conditions. The thermogenesis curves of energy release of the rice mitochondria (which had been kept at 0-3 degrees C for 15 h and 40 day before the determination) were determined respectively at 25 and 30 degrees C, and the difference in shape of the thermogenesis curves and thermodynamic and kinetic characteristics were compared. The thermodynamic and kinetic parameters of energy release of the mitochondria in the thermogenesis increasing stage have been calculated, and the experimental thermokinetic equations of the thermogenesis have been established. The results indicated that the lower the temperature, the slower the energy release of the rice mitochondria. Both the thermogenesis and the energy release rate of the rice mitochondria increased after the mitochondria was kept at lower temperature for 40 days. One can use the methods to characterize the ability of the rice mitochondria to release energy under different conditions.
College of Chemistry and Environmental Sciences, Wuhan University, 430072, Wuhan, China.
P J
PJ
Zhou
H T
HT
Zhou
Y
Y
Liu
S S
SS
Qu
Y G
YG
Zhu
Z B
ZB
Wu
D002151:Calorimetry / Q000295:instrumentation / Q000379:methods*; D004734:Energy Metabolism; D007700:Kinetics; D008928:Mitochondria / Q000378:metabolism*; D012275:Oryza / Q000378:metabolism*; D013816:Thermodynamics