[The effect of c-myc antisense oligodeoxynucleotides on proliferation of pulmonary arterial smooth muscle cells stimulated by hypoxic endothelial cells conditioned medium].
OBJECTIVE
To study the effect of c-myc antisense oligodeoxynucleotides on proliferation of pulmonary arterial smooth muscle cells (SMC) stimulated by hypoxic endothelial cells conditioned medium (HECCM).


METHODS
After pulmonary arterial SMC were stimulated by HECCM, Northern blot technique was used for detection of expression of c-myc, and 3H-TdR incorporation and cell growth assay for detection of proliferation of SMC.


RESULTS
HECCM promoted the expression of c-myc and proliferation of SMC significantly. Antisense-ODNs significantly inhibited the expression of c-myc and proliferation of SMC stimulated by HECCM, whereas sense ODNs didn't affect the expression of c-myc and proliferation of SMC.


CONCLUSIONS
HECCM promote the proliferation of SMC by increasing the expression of c-myc, antisense ODNs inhibits the proliferation of SMC by downregulating the expression of c-myc gene.
Tongji Hospital, Tongji Medical University, Wuhan 430030.
Q
Q
Tao
Z
Z
Zhang
D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D015687:Cell Hypoxia; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology; D015536:Down-Regulation; D004730:Endothelium, Vascular / Q000166:cytology*; D015870:Gene Expression / Q000187:drug effects; D016259:Genes, myc; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*; D011651:Pulmonary Artery / Q000166:cytology
D017077:Culture Media, Conditioned; D020319:Oligodeoxyribonucleotides, Antisense