[Use of toxic fraction isolated from Algerian Androctonus australis hector scorpion venom for the assessment of anti-venom serum].
Today, serotherapy is the only specific treatment used against the scorpionique envenomation. This treatment was associated with other drugs as a symptomatic and supportive care. The anti-venom against scorpion stings is still prepared using the conventional manufacturing production. To evaluate the efficacy of the anti-venom, different protocols are used: The immunization of animals (rabbit and horse) with a whole toxic fraction prepared by molecular filtration was the first step to improve the anti-venom. Some observations also show the need for the quality control of this preparation of anti-venom. In this study, we describe the use the toxic fraction isolated from Androctonus australis Hector in the new protocol of anti-venom production. The quality control of the product is evaluated by using a combination of experimental immunological assay systems (ELISA, Hemaglutination, in vivo assessment of the neutralization of the lethal effect of the venom).
Laraba-Djebari
F
F
Laboratoire de Recherche et Développement sur les venins, Institut Pasteur d'Algérie.
Hammoudi
D
D
D000462:Algeria; D000818:Animals; D000997:Antivenins / Q000276:immunology*; D012604:Scorpion Venoms / Q000276:immunology*; D013045:Species Specificity
D000997:Antivenins; D012604:Scorpion Venoms