Evolutionary dynamics of the T-cell receptor VB gene family as inferred from the human and mouse genomic sequences.
The diversity of T-cell receptors is generated primarily by the variable-region gene families, each of which is composed of a large number of member genes. The entire genomic sequence of the variable region (VB) of the T- cell receptor beta chain from humans and mice has become available. To understand the evolutionary dynamics of the VB gene family, we conducted a phylogenetic analysis of all VB genes from humans and mice, as well as a detailed analysis of internal DNA duplications in the human genomic VB region. The phylogenetic tree obtained shows that human and mouse VB genes intermingle extensively rather than forming two separate clusters and that many gene duplications occurred both before and after the divergence between primates and rodents. Analyzing the genomic maps of transposable elements (e.g., LINEs and SINEs) and relic VB genes in the VB gene region, we present evidence that a 20-kb VB region duplicated tandemly four times in the human lineage during the last 32 Myr, and 6 out of the 15 VB genes in this region have become nonfunctional during this period. Our results show that the VB gene family is subject to evolution by a birth-and-death process rather than to concerted evolution.
Institute of Molecular Evolutionary Genetics and Department of Biology, Pennsylvania State University, USA. su_chen@lilly.com
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D000595:Amino Acid Sequence; D000818:Animals; D020451:Contig Mapping; D019143:Evolution, Molecular; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000145:classification / Q000235:genetics*; D012641:Selection, Genetic; D016415:Sequence Alignment
D016693:Receptors, Antigen, T-Cell, alpha-beta