Restricted growth of ent(-) and tonB mutants of Salmonella enterica serovar Typhi in human Mono Mac 6 monocytic cells.
Monocytes and macrophages are an important host defense in humans infected with Salmonella enterica serovar Typhi. Bacterial ability to survive in these cells is therefore a crucial virulence characteristic of this pathogen. In this study, we demonstrate that growth of a Salmonella enterica serovar Typhi enterochelin synthesis mutant and a tonB mutant in the human monocyte cell line Mono Mac 6 is restricted compared to that of the parental wild-type Ty2 strain. These results suggest that enterochelin- and TonB-mediated iron uptake plays a role in S. enterica serovar Typhi pathogenesis, and also suggest that mutations in iron uptake may attenuate S. enterica serovar Typhi strains for human beings.
Department of Microbiology and Immunology, New York Medical College, Valhalla, NY 10595-1690, USA.
V Y
VY
Gorbacheva
G
G
Faundez
H P
HP
Godfrey
F C
FC
Cabello
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport; D002460:Cell Line; D004251:DNA Transposable Elements; D004758:Enterobactin / Q000378:metabolism*; D005798:Genes, Bacterial; D006801:Humans; D007501:Iron / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009000:Monocytes / Q000382:microbiology*; D009154:Mutation; D012485:Salmonella typhi / Q000235:genetics* / Q000254:growth & development* / Q000472:pathogenicity; D014774:Virulence
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D008565:Membrane Proteins; C057616:tonB protein, Bacteria; D004758:Enterobactin; D007501:Iron