Generation and characterization of a defined mutant of Streptococcus suis lacking suilysin.
A defined allelic-replacement mutant of the sly gene, encoding a thiol-activated cytolysin, from a European isolate of Streptococcus suis serotype 2 was generated and characterized. Unlike the parental strain, it is nonhemolytic, noncytotoxic for cultured macrophage-like cells, avirulent in a mouse infection model, yet only slightly attenuated in a porcine model of systemic infection.
Allen
A G
AG
Centre for Veterinary Science, Department of Clinical Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES, United Kingdom.
Bolitho
S
S
Lindsay
H
H
Khan
S
S
Bryant
C
C
Norton
P
P
Ward
P
P
Leigh
J
J
Morgan
J
J
Riches
H
H
Eastty
S
S
Maskell
D
D
D000818:Animals; D002460:Cell Line; D005260:Female; D006460:Hemolysin Proteins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D009930:Organic Chemicals; D010641:Phenotype; D017011:Streptococcus suis / Q000235:genetics / Q000472:pathogenicity*; D013552:Swine
D006460:Hemolysin Proteins; D009930:Organic Chemicals; C104760:suilysin