Cloning and analysis of valerophenone synthase gene expressed specifically in lupulin gland of hop (Humulus lupulus L.).
Resin and essential oil derived from hop (Humulus lupulus L.) cones are very important compounds for beer brewing, and they specifically accumulate in the lupulin gland of hop cones. In order to identify the genes responsible for the biosynthetic pathway of these compounds and use the identified genes for hop breeding using Marker Assisted Selection and transformation techniques, genes expressed specifically in the lupulin gland were cloned and sequenced. One of them was suggested to be similar to the chalcone synthase gene from the DNA sequence. The translation product of the gene had the activity of valerophenone synthase, which catalyzes a part of the synthesis reaction of alpha-acid and beta-acid. Northern analysis showed that the valerophenone synthase gene seemed to be expressed specifically in the lupulin gland.
Okada
Y
Y
Plant Bioengineering Research Laboratories, Sapporo Breweries Ltd., Gumma, Japan. yukio.okada@sapporobeer.co.jp
Ito
K
K
D000217:Acyltransferases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001515:Beer; D015152:Blotting, Northern; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D003001:Cloning, Molecular; D017343:Genes, Plant; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009822:Oils, Volatile / Q000737:chemistry / Q000378:metabolism*; D018514:Plant Structures / Q000737:chemistry / Q000201:enzymology / Q000235:genetics; D012116:Resins, Plant / Q000737:chemistry / Q000378:metabolism*; D019685:Rosales / Q000201:enzymology / Q000235:genetics* / Q000502:physiology; D016415:Sequence Alignment
D009822:Oils, Volatile; D012116:Resins, Plant; D000217:Acyltransferases; C432588:isovaleryl-CoA malonyl-CoA acyltransferase