Apoptosis-inducing activity of a driselase digest fraction of green tea residue.
We enzymatically digested green tea residue with Driselase, a crude preparation containing cellulase, pectinase and proteases, in order to examine the potential usefulness of the residue. A fraction of the digest soluble in 70% ethanol was found to induce the death of U937 human histiocytic lymphoma cells by apoptosis. Other enzyme preparations gave similar products with cell death-inducing activity of varing potency. The green tea residue may therefore be a useful source of potential agents with anti-cancer activity.
School of Food and Nutritional Sciences, University of Shizuoka, Yada, Japan.
Y
Y
Katsuno
Y
Y
Koyama
K
K
Saeki
M
M
Sazuka
K
K
Ookawa
M
M
Isemura
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis; D053938:DNA Fragmentation; D004354:Drug Screening Assays, Antitumor; D005656:Fungal Proteins; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006801:Humans; D008223:Lymphoma; D013662:Tea / Q000737:chemistry*; D014407:Tumor Cells, Cultured
D000972:Antineoplastic Agents, Phytogenic; D005656:Fungal Proteins; D013662:Tea; D006026:Glycoside Hydrolases; C035212:driselase