New TEM variant (TEM-92) produced by Proteus mirabilis and Providencia stuartii isolates.
The sequences of the bla(TEM) genes encoding TEM-92 in Proteus mirabilis and Providencia stuartii isolates were determined and were found to be identical. Except for positions 218 (Lys-6) and 512 (Lys-104), the nucleotide sequence of bla(TEM-92) was identical to that of bla(TEM-20), including the sequence of the promoter region harboring a 135-bp deletion combined with a G-162-->T substitution. The deduced amino acid sequence of TEM-92 differed from that of TEM-52 by the presence of a substitution (Gln-6-->Lys) in the peptide signal.
de Champs
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Laboratoire de Bactériologie, Faculté de Médecine, 63001 Clermont-Ferrand Cedex, France. Christophe.DESCHAMPS@u-clermont1.fr
Monne
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Bonnet
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Sougakoff
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Sirot
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Chanal
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Sirot
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D019943:Amino Acid Substitution; D004756:Enterobacteriaceae Infections / Q000188:drug therapy / Q000382:microbiology; D005798:Genes, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests; D011401:Promoter Regions, Genetic; D011512:Proteus Infections / Q000188:drug therapy / Q000382:microbiology; D011513:Proteus mirabilis / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D011532:Providencia / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D017422:Sequence Analysis, DNA; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000235:genetics*
C434286:beta-lactamase TEM-92; D001618:beta-Lactamases