A potential double role of anti-Lewis X antibodies in Helicobacter pylori-associated gastroduodenal diseases.
In this study, we found Lewis X (Le(x)) determinants on 68% of Helicobacter pylori isolates from patients with chronic gastroduodenal diseases. Anti-Le(x) IgG were detected more frequently in the sera from dyspeptic children and adults (45 and 46%), with or without proved (culture) H. pylori infection, than in the sera from healthy individuals (14% and 25%). In contrast, the prevalence of anti-Le(x) IgM was higher in the groups of healthy individuals than in the groups of dyspeptic patients. Moreover, anti-Le(x) monoclonal antibody of IgM class enhanced the uptake of Le(x)(+) but not Le(x)(-) H. pylori isolates by phagocytes. In the sera from some dyspeptic patients, we detected Le(x)-anti-Le(x) IgG immune complexes (Le(x) ICs). There was a great difference between children and adults as regards the presence of Le(x) ICs. The immune complexes were found in the sera from nine out of 29 (27%) H. pylori-infected and three out of eight (37%) uninfected adult dyspeptic patients. In comparison, Le(x)-anti-Le(x) IgG ICs were detected only for two out of 18 (11%) H. pylori-infected children. Le(x) ICs were not found in the sera from healthy individuals. Our results suggest that anti-Le(x) IgM may play a protective role in H. pylori infections. In contrast, anti-Le(x) IgG and particularly Le(x)-anti-Le(x) IgG ICs might contribute to the pathogenesis of chronic H. pylori infections.
Department of Infectious Biology, Institute for Microbiology and Immunology, University of Lodz, Poland. rudw@biol.uni.lodz.pl
W
W
Rudnicka
E
E
Czkwianianc
I
I
Płaneta-Małecka
M
M
Jurkiewicz
M
M
Wiśniewska
T
T
Cieślikowski
B
B
Rózalska
T
T
Wadström
M
M
Chmiela
D000293:Adolescent; D000328:Adult; D000368:Aged; D000942:Antigens, Bacterial; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000276:immunology; D002648:Child; D004415:Dyspepsia / Q000276:immunology; D016481:Helicobacter Infections / Q000150:complications / Q000276:immunology*; D016480:Helicobacter pylori / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D016256:Lewis X Antigen / Q000096:biosynthesis / Q000276:immunology*; D008875:Middle Aged; D010437:Peptic Ulcer / Q000150:complications / Q000276:immunology*; D010587:Phagocytosis / Q000276:immunology
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007074:Immunoglobulin G; D016256:Lewis X Antigen; C080866:cagA protein, Helicobacter pylori