A rapid method for the separation and analysis of carrageenan oligosaccharides released by iota- and kappa -carrageenase.
Based on the improved performances in speed of chromatographic separation on Superdex-type materials (Pharmacia) compared to conventional media such as Sephadex and Bio Gel-type, a rapid size-exclusion chromatography (SEC) method was developed for the separation and analysis of carrageenan oligosaccharides. It was used to evaluate the elution profiles of hydrolysates produced by carrageenases specific for kappa- and iota-carrageenans. Oligosaccharide peaks ranging from di- to dodeca-saccharides were obtained in about 20 min on an analytical scale, whereas preparative runs were completed in a few hours. The method may also be used to monitor polysaccharide degradation.
Institute of Chemistry and Biotechnology, Agricultural University of Norway, As.
S H
SH
Knutsen
M
M
Sletmoen
T
T
Kristensen
T
T
Barbeyron
B
B
Kloareg
P
P
Potin
D001426:Bacterial Proteins; D002351:Carrageenan / Q000737:chemistry* / Q000378:metabolism; D002850:Chromatography, Gel / Q000379:methods; D006026:Glycoside Hydrolases / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism; D019906:Nuclear Magnetic Resonance, Biomolecular; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D011549:Pseudomonas / Q000201:enzymology; D021241:Spectrometry, Mass, Electrospray Ionization
D001426:Bacterial Proteins; D007527:Isoenzymes; D009844:Oligosaccharides; D002351:Carrageenan; D006026:Glycoside Hydrolases; C019846:kappa-carrageenase protein, Alteromonadaceae