Protection of dopaminergic antagonists against anoxia-induced inhibition of Ca(2+)-calmodulin dependent protein kinase II activity in rat brain.
OBJECTIVE
To study the effect of dopamine receptor antagonists on anoxia-induced inhibition of Ca(2+)-calmodulin dependent protein kinase II (CCDPK II) activity in rat hippocampus and striatum.


METHODS
Using the rat hippocampal and striatal slices under 95% N2 + 5% CO2, the activity of CCDPK II was examined by 32P-incorporation.


RESULTS
Under anoxia for 30 min, the CCDPK II activity decreased to 29.2% and 27.0% of the control in rat hippocampal and striatal slices, respectively. Preincubation with Sch-23390 (a specific D1-like dopamine receptor antagonist), or domperidone (a specific D2-like dopamine receptor antagonist), resulted in a concentration-dependent attenuation of the anoxia-induced inhibition of CCDPK II activity which was preserved up to about 60%.


CONCLUSIONS
Dopamine receptor stimulation is involved in anoxia-induced inhibition of CCDPK II activity in rat hippocampus and striatum.
Research Center of Biochemistry and Molecular Biology, Xuzhou Medical College, Xuzhou 221002, China.
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Hou
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Zhang
D000818:Animals; D001552:Benzazepines / Q000494:pharmacology; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D015687:Cell Hypoxia; D003342:Corpus Striatum / Q000201:enzymology*; D004294:Domperidone / Q000494:pharmacology; D018492:Dopamine Antagonists / Q000494:pharmacology*; D065127:Dopamine D2 Receptor Antagonists; D006624:Hippocampus / Q000201:enzymology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D017447:Receptors, Dopamine D1 / Q000037:antagonists & inhibitors
D001552:Benzazepines; D018492:Dopamine Antagonists; D065127:Dopamine D2 Receptor Antagonists; D017447:Receptors, Dopamine D1; D004294:Domperidone; D017871:Calcium-Calmodulin-Dependent Protein Kinases