Characterization of apo and partially saturated states of calerythrin, an EF-hand protein from S. erythraea: a molten globule when deprived of Ca(2+).
Calerythrin, a four-EF-hand calcium-binding protein from Saccharopolyspora erythraea, exists in an equilibrium between ordered and less ordered states with slow exchange kinetics when deprived of Ca(2+) and at low temperatures, as observed by NMR. As the temperature is raised, signal dispersion in NMR spectra reduces, and intensity of near-UV CD bands decreases. Yet far-UV CD spectra indicate only a small decrease in the amount of secondary structure, and SAXS data show that no significant change occurs in the overall size and shape of the protein. Thus, at elevated temperatures, the equilibrium is shifted toward a state with characteristics of a molten globule. The fully structured state is reached by Ca(2+)-titration. Calcium first binds cooperatively to the C-terminal sites 3 and 4 and then to the N-terminal site 1, which is paired with an atypical, nonbinding site 2. EF-hand 2 still folds together with the C-terminal half of the protein, as deduced from the order of appearance of backbone amide cross peaks in the NMR spectra of partially Ca(2+)-saturated states.
Institute of Biotechnology/NMR laboratory, FIN-00014 University of Helsinki, Helsinki, Finland. helena.aitio@helsinki.fi
H
H
Aitio
T
T
Laakso
T
T
Pihlajamaa
M
M
Torkkeli
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I
Kilpeläinen
T
T
Drakenberg
R
R
Serimaa
A
A
Annila
D001426:Bacterial Proteins; D002118:Calcium / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D002942:Circular Dichroism; D018360:Crystallography, X-Ray; D020832:EF Hand Motifs; D009682:Magnetic Resonance Spectroscopy; D016930:Saccharopolyspora / Q000737:chemistry* / Q000378:metabolism; D017186:Titrimetry
D001426:Bacterial Proteins; D002135:Calcium-Binding Proteins; C090958:calerythrin protein, Saccharopolyspora erythraea; D002118:Calcium