Changes in quantitative profile of extracellular matrix components in the kidneys of rats with adriamycin-induced nephropathy.
Extracellular matrix components (ECMs) in histological sections of the kidney cortex from the rats with adriamycin (ADR)-induced nephropathy (5 mg/kg, i.v.) were quantified by an immunohistochemical micromethod. Changes in kidney histopathology and urine and blood biochemistry were investigated. Enlarged kidneys were granular on the surface and pale in color in ADR-treated rats, and these rats had kidneys with glomeruli with expanded mesangial area and with capillary aneurysm. Severe albuminuria, hypoalbuminemia, hypercholesterolemia and disorders in other nephrotic parameters were observed in ADR-treated rats. Type I and IV collagens, fibronectin and laminin contents in the renal cortex of ADR-treated rats at 10 weeks were 329, 317, 263 and 295%, respectively, higher than in each vehicle control, and those at 28 weeks were 1,211, 930, 1,057 and 1,012%, respectively. The glomerular sclerotic abnormalities progressed in a time-dependent manner. Moreover, there was a strong correlation between the ECM levels and serum creatinine and blood urea nitrogen levels. In conclusion, microquantification provided useful information for accurate diagnosis and prognosis of nephrotic lesions and is a good tool to assess the advancement of renal disorders in patients with nephropathy.
Department of Animal Sciences, Kyoto University, Japan.
N
N
Manabe
A
A
Kinoshita
M
M
Yamaguchi
Y
Y
Furuya
N
N
Nagano
K
K
Yamada-Uchio
N
N
Akashi
K
K
Miyamoto-Kuramitsu
H
H
Miyamoto
D000419:Albuminuria / Q000652:urine; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000633:toxicity*; D001806:Blood Urea Nitrogen; D002784:Cholesterol / Q000097:blood; D003094:Collagen / Q000378:metabolism; D003404:Creatinine / Q000097:blood; D004317:Doxorubicin / Q000633:toxicity*; D005109:Extracellular Matrix / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D005923:Glomerulosclerosis, Focal Segmental / Q000139:chemically induced / Q000473:pathology / Q000662:veterinary*; D007150:Immunohistochemistry / Q000662:veterinary; D007797:Laminin / Q000378:metabolism; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D012376:Rodent Diseases / Q000139:chemically induced / Q000473:pathology*; D012709:Serum Albumin / Q000032:analysis; D018709:Statistics, Nonparametric
D000903:Antibiotics, Antineoplastic; D005353:Fibronectins; D007797:Laminin; D012709:Serum Albumin; D004317:Doxorubicin; D003094:Collagen; D002784:Cholesterol; D003404:Creatinine