Access to the inaccessible sequence of cpn 60.1 (195-217) by temporary oxazolidine protection of selected amide bonds.
The solid-phase peptide synthesis of a reportedly inaccessible peptide sequence of chaperonin 60.1 (195-219) is described using oxazolidine containing dipeptide building blocks ('pseudo-proline' dipeptide units). Two attempts at the synthesis of the chaperonin 60.1 sequence are outlined using one and two pseudo-proline units, respectively, and these results are compared with the outcome of an ordinary stepwise (double) coupling procedure. The only successful synthesis is that combining two pseudo-proline building blocks.
Imperial College Genetic Therapies Centre, Department of Chemistry, Imperial College of Science, Technology and Medicine, South Kensington, UK. mike.keller@ic.ac.uk
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Miller
D000577:Amides / Q000737:chemistry; D018834:Chaperonin 60 / Q000138:chemical synthesis* / Q000737:chemistry; D004151:Dipeptides / Q000737:chemistry; D010080:Oxazoles / Q000737:chemistry*; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry
D000577:Amides; D018834:Chaperonin 60; D004151:Dipeptides; D010080:Oxazoles; D010446:Peptide Fragments; C064210:oxazolidine