[Effect of the herpes simplex virus I-thymidine kinase gene-ganciclovir system on the transplant of human ovarian cancer on the omentum of nude mice].
OBJECTIVE
To investigate the therapeutic effect of the herpes simplex virus I-thymidine kinase gene (HSV1-tk)/ganciclovir (GCV) system on human ovarian cancer.


METHODS
The nude mice tumors were formed by injection of human ovarian cancer cell line AO and AO/HSV1-tkc (AO cells carried with HSV1-tk gene which was constructed in China) subcutaneously, and then were transplanted to the omentum of nude mice. The filter-passing culture fluid of VPC/HSV1-tkc was injected daily into the peritoneum of the nude mice in the group of in vivo. Finally, all of the three groups of nude mice (control, in vivo, ex vivo) accepted the treatment of GCV.


RESULTS
The inhibitory rate of the RV/HSV1-tkc/GCV to AO tumors were 46.8%, and, only could the residual microscopic tumors be seen in most of the nude mice omentum transplanted with AO/HSV1-tkc tumor after GCV treatment.


CONCLUSIONS
GCV could more effectively inhibit the growth of the human ovarian tumors carried with HSV1-tk gene which was transplanted onto the nude mice omentum than which transplanted subcutaneously; The application of the HSV1-tk/GCV system would be very promising in the gene therapy of ovarian cancer.
Xu
C
C
Gynecology and Obstetrics Hospital of Shanghai Medical University, Shanghai, 200011.
Zhang
X
X
Jin
Z
Z
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D005260:Female; D015774:Ganciclovir / Q000494:pharmacology; D015316:Genetic Therapy; D018259:Herpesvirus 1, Human / Q000201:enzymology* / Q000235:genetics; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009852:Omentum; D010051:Ovarian Neoplasms / Q000628:therapy*; D013937:Thymidine Kinase / Q000235:genetics*; D014162:Transfection
D000998:Antiviral Agents; D013937:Thymidine Kinase; D015774:Ganciclovir