An improved method for extraction of beta-carotene from Blakeslea trispora.
An improved method for the extraction of beta-carotene from Blakeslea trispora is described. The fermentation broth was steamed at 121 degrees C for 15 min, and the liquid was centrifuged at 5000 g for 20 min. beta-Carotene was removed from the biomass by extraction with absolute ethanol at a ratio of 1:100 at 30 degrees C for 2 h in a rotary shaker incubator at 300 rpm. The carotenoid pigment was completely removed from the cells after three repeated extractions. The removal of beta-carotene from B. trispora was higher during the first stage (75%) whereas in the other stages it was very slow.
Department of Food Science and Technology, Aristotle University of Thessaloniki, Box 250, 540 06, Thessaloniki, Greece. roukas@agro.auth.gr
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Roukas
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Mantzouridou
D019149:Bioreactors; D000431:Ethanol; D005285:Fermentation; D005658:Fungi / Q000737:chemistry* / Q000254:growth & development; D006358:Hot Temperature; D012997:Solvents; D013997:Time Factors; D019207:beta Carotene / Q000302:isolation & purification*
D012997:Solvents; D019207:beta Carotene; D000431:Ethanol