[Effect of HHV-6 on expression of M-CSF in patients with hematological disorders].
OBJECTIVE
To study the relationship between HHV-6 infection and abnormal expression of M-CSF in bone marrow mononucleated cells (MNC) from patients with hematological disorders.


METHODS
M-CSF expression level were detected in 30 specimens of MNC from patients with hematological diseases by ABC immunocytochemical staining and reverse transcription polymerase chain reaction methods.


RESULTS
The percentage and score of M-CSF antigen positive cells, and percentage of M-CSF mRNA expressing samples derived from HHV-6 infected group were 36.9% +/- 24.9%, 45.9 +/- 31.4 and 83.3%, respectively, which were significantly higher than those of control (20.3% +/- 19.4%, 25.3 +/- 24.2 and 50.0%, respectively) (P < 0.001). HHV-6 could promote M-CSF production to a large extent in MNC from leukemic patients than that from patients with benign hematological disorders. M-CSF existed mainly on membrane or in cytosol of granulocytes and monocytes.


CONCLUSIONS
HHV-6 infection in vitro could promote M-CSF production in MNC from patients with hematological diseases.
Institute of Hematology, CAMS and PUMC, Tianjin 300020.
W
W
Yang
K
K
Wu
Y
Y
Song
D000328:Adult; D005260:Female; D006566:Herpesviridae Infections / Q000378:metabolism*; D015654:Herpesvirus 6, Human; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000821:virology*; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D016173:Macrophage Colony-Stimulating Factor / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged
D016173:Macrophage Colony-Stimulating Factor