Autoradiography with [3H]PK11195 of spinal tract degeneration in amyotrophic lateral sclerosis.
The diagnostic hallmarks of amyotrophic lateral sclerosis (ALS) are degeneration of upper and lower motor neurons and of corticospinal tracts. Here, we demonstrate the suitability of the gliosis marker [3H]PK11195 for quantitative evaluation of tract degeneration in ALS in vitro. Binding of [3H]PK11195 was increased in lateral and ventral white matter of ALS spinal cords but not in the anterior horn, in spite of a dramatic loss in muscarinic binding sites and a high level of oxidatively modified protein. Labeling of activated microglia with [11C]PK11195 may also allow tract degeneration in ALS to be visualized in vivo.
Institute of Biochemical Pharmacology, University of Vienna, Austria.
H H
HH
Sitte
J
J
Wanschitz
H
H
Budka
M L
ML
Berger
D000328:Adult; D000368:Aged; D000690:Amyotrophic Lateral Sclerosis / Q000473:pathology* / Q000503:physiopathology; D000970:Antineoplastic Agents; D001345:Autoradiography; D001665:Binding Sites / Q000187:drug effects / Q000502:physiology; D015415:Biomarkers / Q000032:analysis; D005911:Gliosis / Q000473:pathology* / Q000503:physiopathology; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D007546:Isoquinolines; D017628:Microglia / Q000187:drug effects / Q000378:metabolism; D008875:Middle Aged; D009410:Nerve Degeneration / Q000473:pathology* / Q000503:physiopathology; D018384:Oxidative Stress / Q000502:physiology; D011712:Pyramidal Tracts / Q000473:pathology* / Q000503:physiopathology; D011963:Receptors, GABA-A / Q000187:drug effects / Q000378:metabolism; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000378:metabolism; D014316:Tritium
D000970:Antineoplastic Agents; D015415:Biomarkers; D006684:HLA-DR Antigens; D007546:Isoquinolines; D011963:Receptors, GABA-A; D011976:Receptors, Muscarinic; D014316:Tritium; C037850:PK 11195