Artemether administered together with haemin damages schistosomes in vitro.
We conducted experiments in vitro to assess the effect of artemether in combination with haemin on adult Schistosoma japonicum, S. mansoni and S. haematobium. When schistosomes were maintained in a medium containing artemether at concentrations of 20 micrograms/mL or less for 72 h, no apparent effect on the schistosomes was seen. When the medium contained 50 or 100 micrograms/mL haemin as well as artemether, the schistosomes showed decreased motor activity 2-24 h after exposure, which was followed by the staining of the whole worm body a reddish-yellow colour, dilatation of the intestine, and extensive vesiculation of the tegument. Some of the schistosomes died 24 h after exposure, and almost all died within 48-72 h. When schistosomes were exposed to the same concentrations of haemin alone, they were stained a light yellow colour but there was no apparent effect on their survival. Our findings suggest that artemether interacts with haemin to exert a toxic effect on the worms, which might be of importance in the further elucidation of the mechanism of action of artemether on schistosomes.
Xiao
S
S
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025, China.
Chollet
J
J
Utzinger
J
J
Matile
H
H
Mei
J
J
Tanner
M
M
D000818:Animals; D000981:Antiprotozoal Agents / Q000627:therapeutic use*; D000077549:Artemether; D037621:Artemisinins; D004338:Drug Combinations; D005260:Female; D006427:Hemin / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D021261:Parasitic Sensitivity Tests; D011092:Polyethylene Glycols / Q000627:therapeutic use; D012548:Schistosoma haematobium / Q000187:drug effects; D012549:Schistosoma japonicum / Q000187:drug effects; D012550:Schistosoma mansoni / Q000187:drug effects; D012553:Schistosomiasis haematobia / Q000188:drug therapy*; D012554:Schistosomiasis japonica / Q000188:drug therapy*; D012555:Schistosomiasis mansoni / Q000188:drug therapy*; D012717:Sesquiterpenes / Q000627:therapeutic use*
D000981:Antiprotozoal Agents; D037621:Artemisinins; D004338:Drug Combinations; D012717:Sesquiterpenes; D011092:Polyethylene Glycols; D006427:Hemin; D000077549:Artemether