Mutagenicity and genotoxicity of sorbic acid-amine reaction products.
Sorbic acid (E200) and its salts (potassium and calcium sorbate: E202 and E203) are allowed for use as preservatives in numerous processed foods. Sorbic acid had a conjugated system of double bonds which makes it susceptible to nucleophilic attack, sometimes giving mutagenic products. Under conditions typical of food processing (50-80 degrees C), we analysed the cyclic derivatives resulting from a double addition reaction between sorbic acid and various amines. Mutagenesis studies, involving Ames' test and genotoxicity studies with HeLa cells and plasmid DNA, showed that none of the products studied presented either mutagenic or genotoxic activities.
Laboratoire d'Analyse de la Valeur Biologique des Aliments, Departement Universitaire des Sciences d'Agen, Université Bordeaux 1, avenue Michel Serres, 47000 Agen, France. dusa@dusagen.u-bordeaux.fr
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Ferrand
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Marc
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Fritsch
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Cassand
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de Saint Blanquat
D000588:Amines / Q000032:analysis* / Q000737:chemistry; D002849:Chromatography, Gas; D005511:Food Handling / Q000379:methods*; D005520:Food Preservatives / Q000032:analysis*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D009152:Mutagenicity Tests; D009153:Mutagens / Q000032:analysis* / Q000737:chemistry; D013011:Sorbic Acid / Q000031:analogs & derivatives / Q000032:analysis* / Q000737:chemistry
D000588:Amines; D005520:Food Preservatives; D009153:Mutagens; D013011:Sorbic Acid