Contrasting effect of interleukin-4 on the expression of cytosolic phospholipase A2, 5-lipoxygenase and 5-lipoxygenase-activating protein in peritoneal macrophages from control and ovalbumin-sensitized rats.
Interleukin-4 (IL-4), which has been widely described as an anti-inflammatory cytokine, can also exert proinflammatory effects. In this study, we extend these findings to demonstrate, in an allergic model, the dual effect of IL-4 on arachidonic acid (AA) metabolism in macrophages. In peritoneal macrophages from control rats (cPM), IL-4 had no effect on cPLA2 and 5-LO expression, but increased FLAP expression without affecting basal and A23187- or PMA-challenged arachidonic acid (AA) metabolism. In contrast, in peritoneal macrophages from ovalbumin-sensitized rats (sPM), IL-4 decreased cPLA2, 5-LO and FLAP expression and PMA-challenged eicosanoid production. A23187-challenged AA metabolism of sPM was not affected by IL-4 pretreatment. Thus, IL-4 acts differently on cPLA2, 5-LO and FLAP expression and AA metabolism in peritoneal macrophages depending on their resident or sensitization-induced differentiated status.
Université Paul-Sabatier UPRES EA 2405, Macrophages, Médiateurs de l'Inflammation et Interactions Cellulaires, INSERM IFR31, CHU Rangueil, 1, avenue Jean-Poulhès, 31403 Toulouse Cedex 4, France.
L
L
Escoubet-Lozach
C F
CF
M'Rini
A
A
Rey
M
M
Béraud
J C
JC
Lepert
J
J
Bernard
J R
JR
Mathieu
B
B
Pipy
D058927:5-Lipoxygenase-Activating Proteins; D000818:Animals; D001094:Arachidonate 5-Lipoxygenase / Q000235:genetics*; D016718:Arachidonic Acid / Q000378:metabolism; D000001:Calcimycin / Q000494:pharmacology; D002352:Carrier Proteins / Q000235:genetics*; D002788:Cholesterol Esters / Q000378:metabolism; D003600:Cytosol / Q000201:enzymology; D005786:Gene Expression Regulation / Q000187:drug effects*; D015847:Interleukin-4 / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D010047:Ovalbumin / Q000008:administration & dosage*; D010741:Phospholipases A / Q000235:genetics*; D054467:Phospholipases A2; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D058927:5-Lipoxygenase-Activating Proteins; D002352:Carrier Proteins; D002788:Cholesterol Esters; D008565:Membrane Proteins; D010743:Phospholipids; D015847:Interleukin-4; D016718:Arachidonic Acid; D000001:Calcimycin; D010047:Ovalbumin; D001094:Arachidonate 5-Lipoxygenase; D010741:Phospholipases A; D054467:Phospholipases A2; D013755:Tetradecanoylphorbol Acetate