Population pharmacokinetics of antifibroblast activation protein monoclonal antibody F19 in cancer patients.
OBJECTIVE
The population pharmacokinetics of 131I-mAbF19, a radiolabelled murine monoclonal antibody against fibroblast activation protein and a potential antitumour stroma agent, were investigated during two phase I studies in cancer patients.


METHODS
131I-mAbF19 serum concentration-time data were obtained in 16 patients from two studies involving imaging and dosimetry in colorectal carcinoma and soft tissue sarcoma. Doses of 0.2, 1 and 2 mg antibody were administered as 60 min intravenous infusions. The data were analysed by nonlinear mixed effect modelling.


RESULTS
The data were described by a two-compartment model. Population mean values were 109 ml h(-1) for total serum clearance, 3.1 l for the volume of distribution of the central compartment, and 4.9 l for the volume of distribution at steady state. Mean terminal half-life was 38 h. Intersubject variability was high, but no patient covariates could be identified that further explained this variability. In particular, there was no influence of tumour type or mAbF19 dose.


CONCLUSIONS
The pharmacokinetics of antistromal mAbF19 were well defined in these two studies with different solid tumour types, and were comparable with those of other murine monoclonal antibodies that do not bind to normal tissue antigens or blood cells.
Tanswell
P
P
Department of Pharmacokinetics and Drug Metabolism, Boehringer Ingelheim Pharma KG, Biberach, Germany. paul.tanswell@bc.boehringer-ingelheim.com
Garin-Chesa
P
P
Rettig
W J
WJ
Welt
S
S
Divgi
C R
CR
Casper
E S
ES
Finn
R D
RD
Larson
S M
SM
Old
L J
LJ
Scott
A M
AM
D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal / Q000097:blood / Q000493:pharmacokinetics*; D000951:Antigens, Neoplasm; D000970:Antineoplastic Agents / Q000097:blood / Q000493:pharmacokinetics*; D014408:Biomarkers, Tumor; D010450:Endopeptidases; D005260:Female; D018093:Gelatinases; D006133:Growth Substances / Q000276:immunology*; D006801:Humans; D008297:Male; D008565:Membrane Proteins; D008875:Middle Aged; D009369:Neoplasms / Q000378:metabolism*; D012697:Serine Endopeptidases / Q000276:immunology*; D000099167:Fibroblast Activation Protein Alpha
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000970:Antineoplastic Agents; D014408:Biomarkers, Tumor; D010450:Endopeptidases; D018093:Gelatinases; D006133:Growth Substances; D008565:Membrane Proteins; D012697:Serine Endopeptidases; D000099167:Fibroblast Activation Protein Alpha