Role of lycopene in recovery of radiation induced injury to mammalian cellular organelles.
Whole body exposure of male rats to 7 Gy gamma irradiation increased lipid peroxidation in the liver resulting in biomembrane damage of subcellular structures and release of their enzymes. This is evidenced by increase of thiobarbituric acid-reactive substances (TBARS) in mitochondria, lysosomes and microsomes. This was associated with a decrease in activity of the enzymes specific for each subcellular fraction; namely, mitochondrial glutamate dehydrogenase (GDH), lysosomal beta-glucuronidase and microsomal glucose 6-phosphatase. This was paralleled by an increased activity of these enzymes in the cytosol. Rats were supplemented with lycopene, a carotenoid present in tomatoes (5 mg/kg weight/day), by gavage, for 7 days before exposure to 7 Gy gamma irradiation. This resulted in diminishing amount of TBARS recorded for each subcellular structure in the liver of irradiated animals. Significant amelioration in the decrease recorded for the activity of mitochondrial glutamate dehydrogenase, lysosomal beta-glucuronidase and microsomal glucose 6-phosphatase was observed. This was associated with significant amelioration in the increase recorded for the activity of these enzymes in the cytosol. It is postulated that lycopene could play an important role in the recovery of the integrity of biological membranes of the liver after radiation injury.
Saada Helen
N
N
Radiation Biology Department, National Center for Radiation Research and Technology, Atomic Energy Authority, Egypt.
Azab Khaled
S
S
D000818:Animals; D002338:Carotenoids / Q000494:pharmacology*; D003600:Cytosol / Q000187:drug effects / Q000528:radiation effects; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D005966:Glucuronidase / Q000378:metabolism; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects; D000077276:Lycopene; D008297:Male; D008566:Membranes / Q000187:drug effects / Q000528:radiation effects; D008862:Microsomes, Liver / Q000187:drug effects / Q000528:radiation effects; D015388:Organelles / Q000187:drug effects* / Q000201:enzymology / Q000528:radiation effects*; D011832:Radiation Injuries / Q000188:drug therapy* / Q000201:enzymology; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D051381:Rats; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014916:Whole-Body Irradiation
D011837:Radiation-Protective Agents; D017392:Thiobarbituric Acid Reactive Substances; D002338:Carotenoids; D005969:Glutamate Dehydrogenase; D005952:Glucose-6-Phosphatase; D005966:Glucuronidase; D000077276:Lycopene