Effect of 17-beta-estradiol and ginsenoside Rg1 on reactive microglia induced by beta-amyloid peptides.
The reactive microglias induced by 25 micromol of beta-amyloid peptides (Abeta25-35) and/or IFN-gamma can initiate the microglial respiratory burst and release NO, respectively. Oxidative stress and inflammatory function have been implicated in Alzheimer's disease (AD). We showed that 10 micromol 17-beta-estradiol (E2) and 1-10 micromol ginsenoside Rg1 (Rg1) could prevent the toxicity of Abeta25-35 and/or IFN-gamma to microglias, inhibit the microglial respiratory burst activity and decrease the accumulation of NO. These results demonstrated the protectional effect of E2 or Rg1 on neuron from damaging by reactive microglias in AD.
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking, Union Medical College, Beijing.
Y S
YS
Gong
J T
JT
Zhang
D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors / Q000633:toxicity*; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002491:Central Nervous System Agents / Q000494:pharmacology*; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects; D004347:Drug Interactions; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004958:Estradiol / Q000494:pharmacology*; D036145:Ginsenosides; D007371:Interferon-gamma / Q000037:antagonists & inhibitors / Q000633:toxicity; D017628:Microglia / Q000187:drug effects* / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D018697:Nootropic Agents / Q000494:pharmacology*; D010446:Peptide Fragments / Q000037:antagonists & inhibitors / Q000633:toxicity*; D051381:Rats; D016897:Respiratory Burst / Q000187:drug effects; D012503:Saponins / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000037:antagonists & inhibitors / Q000633:toxicity
D016229:Amyloid beta-Peptides; D002491:Central Nervous System Agents; D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; D009573:Nitrites; D018697:Nootropic Agents; D010446:Peptide Fragments; D012503:Saponins; C074636:amyloid beta-protein (25-35); D004958:Estradiol; D007371:Interferon-gamma; D013755:Tetradecanoylphorbol Acetate; C035054:ginsenoside Rg1