Exercise training and energy restriction decrease neutrophil phagocytic activity in judoists.
OBJECTIVE
To investigate the effects of weight reduction as the result of exercise training and energy restriction on neutrophil function.


METHODS
Eighteen male competitive college judoists participated in the study. In a whole blood assay, oxidative burst activity, phagocytic activity, expressions of Fc gamma receptor 3 (CD16), and complement receptor 3 (CD11b) of neutrophils were measured on a per cell basis by flow cytometry at day 20, 5, and 1 before and at day 7 after the competition.


RESULTS
The rate of neutrophil producing reactive oxygen species decreased before the competition, whereas the oxidative burst activity per cell increased significantly in all subjects, which resulted in a significant increase of the total oxidative burst activity. However, there were no significant effect of energy restriction on oxidative burst activity. The rate of neutrophils incorporating opsonized zymosan decreased significantly with energy restriction. The total phagocytic activity of 10,000 neutrophils and the phagocytic activity per cell also decreased significantly by severe energy restriction. The surface antigen expressions of CD11b and CD16 were unaffected by weight reduction.


CONCLUSIONS
The results suggest that with respect to the management of health conditions, weight reduction for judoists should be composed of exercise training and energy restriction should be moderate.
Kowatari
K
K
Department of Hygiene, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki, Aomori 036-8562, Japan. kenji@cc.hirosaki-u.ac.jp
Umeda
T
T
Shimoyama
T
T
Nakaji
S
S
Yamamoto
Y
Y
Sugawara
K
K
D000328:Adult; D000704:Analysis of Variance; D001772:Blood Cell Count; D001823:Body Composition; D015444:Exercise / Q000502:physiology*; D005434:Flow Cytometry; D006801:Humans; D016177:Macrophage-1 Antigen / Q000378:metabolism; D008297:Male; D016139:Martial Arts / Q000502:physiology*; D009504:Neutrophils / Q000378:metabolism / Q000502:physiology*; D010587:Phagocytosis; D017382:Reactive Oxygen Species / Q000378:metabolism; D017452:Receptors, IgG / Q000378:metabolism; D015431:Weight Loss / Q000502:physiology
D016177:Macrophage-1 Antigen; D017382:Reactive Oxygen Species; D017452:Receptors, IgG