Expression of heparin/heparan sulfate interacting protein/ribosomal protein l29 during the estrous cycle and early pregnancy in the mouse.
Using a variety of approaches, we have examined the expression of the heparin/heparan sulfate (Hp/HS) interacting protein/ribosomal protein L29 (HIP/RPL29) in mouse uteri during the estrous cycle and early pregnancy. HIP/RPL29 selectively binds heparin and HS and may promote HS-dependent embryo adhesion. HIP/RPL29 was prominently expressed in both luminal and glandular epithelia under almost all conditions, including the phase of embryo attachment. In contrast, differences were noted in HIP/RPL29 expression in the stromal compartment both during the estrous cycle and during early pregnancy. Most notably, HIP/RPL29 accumulated in decidua, where it displayed a pattern complementary to that of pericellular deposition of the HS proteoglycan, perlecan. HIP/RPL29 protein was detected in implanted embryos at both initial and later stages of implantation; however, embryonic HIP/RPL29 mRNA accumulation was more pronounced at later stages (Day 7.5 postcoitum). In situ hybridization revealed similar spatial changes for HIP/RPL29 mRNA during these different physiological states. Whereas differences in the spatial pattern of HIP/RPL29 protein and mRNA expression were demonstrable, little change was detected in the level of HIP/RPL29 mRNA or protein in total endometrial extracts. Mouse blastocysts attached, but did not outgrow, on surfaces coated with recombinant murine HIP/RPL29. Surprisingly, soluble glycosaminoglycans including heparin, low molecular weight heparin, or chondroitin sulfate were not able to inhibit embryo attachment to HIP/RPL29-coated surfaces. These latter observations indicate that embryonic cell surface components other than HS proteoglycans can promote binding to HIP/RPL29 expressed by uterine cells.
Department of Biological Sciences, University of Delaware, Newark 19707, USA.
J
J
Julian
S K
SK
Das
S K
SK
Dey
D
D
Baraniak
V T
VT
Ta
D D
DD
Carson
D000818:Animals; D001755:Blastocyst / Q000378:metabolism; D003656:Decidua / Q000737:chemistry / Q000378:metabolism; D010064:Embryo Implantation; D004622:Embryo, Mammalian / Q000378:metabolism; D004971:Estrus / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006497:Heparan Sulfate / Q000378:metabolism; D017403:In Situ Hybridization; D051379:Mice; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D011994:Recombinant Proteins / Q000378:metabolism; D012269:Ribosomal Proteins / Q000235:genetics* / Q000378:metabolism; D014599:Uterus / Q000737:chemistry / Q000378:metabolism*
D006497:Heparan Sulfate; D012333:RNA, Messenger; D011994:Recombinant Proteins; D012269:Ribosomal Proteins; C035078:ribosomal protein L29