Interaction of metal ions with lupin seed conglutin gamma.
Various metal ions were capable of aggregating and precipitating conglutin gamma, an oligomeric glycoprotein purified from Lupinus albus seeds, at neutral pH values. The most effective metal ions, at 60-fold molar excess to the protein, were Zn2+, Hg2+ and Cu2+; a lower influence on the physical status of conglutin gamma was observed with Cr3+, Fe3+, Co2+, Ni2+, Cd2+, Sn2+, and Pb2+, while Mg2+, Ca2+ and Mn2+ had no effect at all. The insolubilisation of the protein with Zn2+, which is fully reversible, strictly depended on both metal concentration and pH. with middle points of the sharp transitions at three-fold molar excess and pH 6.5, respectively. Conglutin gamma is also fully retained on a metal affinity chromatography column at which Zn2+ and Ni2+ were complexed. A drop of pH below 6.0 and the use of chelating agents, such as EDTA and imidazole, fully desorbed the protein. A slightly lower binding to immobilised Cu2+ and Co2+ and no binding with Mg2+, Cd2+ and Mn2+ were observed. The role of the numerous histidine residues of conglutin gamma in the binding of Zn2+ is discussed.
Duranti
M
M
Dipartimento di Scienze Molecolari Agroalimentari, Università degli Studi di Milano, Italy. marcello.duranti@unimi.it
Scarafoni
A
A
Di Cataldo
A
A
Sessa
F
F
D002412:Cations / Q000737:chemistry; D002413:Cations, Divalent / Q000737:chemistry; D007887:Fabaceae / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008670:Metals / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D010946:Plants, Medicinal; D012639:Seeds / Q000737:chemistry*
D002412:Cations; D002413:Cations, Divalent; D008670:Metals; D010940:Plant Proteins; C079483:conglutin-gamma protein, Lupinus angustifolius