Detection and quantification of depurinated benzo[a]pyrene-adducted DNA bases in the urine of cigarette smokers and women exposed to household coal smoke.
Polycyclic aromatic hydrocarbons (PAH) are metabolized to electrophiles that can bind to DNA bases and destabilize the N-glycosyl bond, causing rapid depurination of the adducted bases. Recent studies support depurination of DNA as a mechanism central to the genesis of H-ras mutations in PAH-treated mouse skin. Depurinating adducts account for 71% of all DNA adducts formed in mouse skin treated with benzo[a]pyrene (BP). This study analyzed urine of cigarette smokers, coal smoke-exposed women, and nonexposed controls for the presence and quantities of the depurinated BP-adducted DNA bases, 7-(benzo[a]pyren-6-yl)guanine (BP-6-N7Gua) and 7-(benzo[a]pyren-6-yl)adenine (BP-6-N7Ade). Since these adducted bases originate from reaction of the BP radical cation with double-stranded DNA and not with RNA or denatured DNA, their presence in urine is indicative of DNA damage. Urine samples were fractionated by a combination of SepPak extraction and reverse-phase HPLC, and then analyzed by tandem mass spectrometry and capillary electrophoresis with laser-induced fluorescence. BP-adducted bases were detected in the urine from three of seven cigarette smokers and three of seven women exposed to coal smoke, but were not detected in urine from the 13 control subjects. Concentrations were estimated to be 60-340 and 0.1-0.6 fmol/mg of creatinine equivalent of urine for coal smoke-exposed women (maximum possible BP intake of ca. 23 000 ng/day) and cigarette smokers (BP intake of ca. 800 ng/day), respectively, exhibiting a sensitive response to BP exposures. BP-6-N7Gua was present at ca. 20-300 times the concentration of BP-6-N7Ade in the urine of coal smoke-exposed women, but was not detected in the urine of cigarette smokers. This difference may be due to the remarkably different BP exposures experienced by the two groups of PAH-exposed individuals. These results justify more extensive studies of depurinated BP-adducted DNA bases as potential biomarkers of PAH-associated cancer risk.
Casale
G P
GP
Eppley Institute for Research in Cancer, 986805, University of Nebraska Medical Center, Omaha, Nebraska 68198-6805, USA.
Singhal
M
M
Bhattacharya
S
S
RamaNathan
R
R
Roberts
K P
KP
Barbacci
D C
DC
Zhao
J
J
Jankowiak
R
R
Gross
M L
ML
Cavalieri
E L
EL
Small
G J
GJ
Rennard
S I
SI
Mumford
J L
JL
Shen
M
M
D000328:Adult; D016902:Air Pollution, Indoor / Q000009:adverse effects*; D001564:Benzo(a)pyrene / Q000633:toxicity*; D002851:Chromatography, High Pressure Liquid; D003031:Coal; D018736:DNA Adducts / Q000737:chemistry / Q000652:urine*; D019075:Electrophoresis, Capillary; D005260:Female; D006801:Humans; D013058:Mass Spectrometry; D008875:Middle Aged; D012906:Smoke / Q000009:adverse effects*; D012907:Smoking / Q000652:urine*; D013050:Spectrometry, Fluorescence
D003031:Coal; D018736:DNA Adducts; D012906:Smoke; D001564:Benzo(a)pyrene