[The influences of interleukin-1 beta converting enzyme expression on the biologic characteristics of ovarian cancer cells].
OBJECTIVE
To investigate the effects of interleukin-1 beta converting enzyme (ICE) gene on the biologic characteristics of ovarian cancer cells.


METHODS
A 1.3 Kilobase human ICE cDNA with 5' and 3' untranslated sequences was cloned into the EcoR I site of pLXSN retrovirus vector in sense orientation. After that, the recombinant plasmid pLXSN-ICE was transferred to virus-packing cell PA317 by electroporation method. And then the retrovirus containing human ICE cDNA generated by these PA317 cells were used to transfect human ovarian cancer cell lines, AO and 3AO.


RESULTS
After being transfected with these retrovirus, the number of the clones obtained after G418 resistance selection is far less than that of controlled groups with plasmid pLXSN. The growth rate of these transfected cells was significantly suppressed in comparison with the parental cell line. Apoptosis was also found in these cells. After the transfection cancer cells were inoculated subcutaneously in nude mice, the tumor growth was significantly inhibited.


CONCLUSIONS
It suggest that human ICE gene can suppress the phenotype and tumorigenicity in nude mice of ovarian cancer cell lines AO and 3AO.
Second Military Medical University, Changhai Hospital, Shanghai 200433.
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Li
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X
Kong
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Qian
D000818:Animals; D017209:Apoptosis; D020170:Caspase 1 / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010051:Ovarian Neoplasms / Q000473:pathology*; D010641:Phenotype; D012190:Retroviridae / Q000235:genetics; D014162:Transfection; D014407:Tumor Cells, Cultured
D020170:Caspase 1