The human cell line PER.C6 provides a new manufacturing system for the production of influenza vaccines.
Influenza viruses for vaccine production are currently grown on embryonated eggs. This manufacturing system conveys many major drawbacks such as inflexibility, cumbersome down stream processing, inability of some strains to replicate on eggs to high enough yields, and selection of receptor-binding variants with reduced antigenicity. These limitations emphasize the need for a cell line-based production system that could replace eggs in the production of influenza virus vaccines in a pandemic proof fashion. Here we present the efficient propagation of influenza A and B viruses on the fully characterized and standardized human cell line PER.C6.
Pau
M G
MG
IntroGene B.V.- Crucell Holland B.V., Archimedesweg 4, 2333 CN, Leiden, The Netherlands. m.g.pau@crucell.com
Ophorst
C
C
Koldijk
M H
MH
Schouten
G
G
Mehtali
M
M
Uytdehaag
F
F
D000818:Animals; D000956:Antigens, Viral / Q000302:isolation & purification; D019149:Bioreactors; D002460:Cell Line; D002645:Chickens; D004492:Edetic Acid; D006389:Hemagglutinins, Viral / Q000302:isolation & purification; D006801:Humans; D007252:Influenza Vaccines / Q000276:immunology / Q000302:isolation & purification*; D009975:Orthomyxoviridae / Q000276:immunology / Q000502:physiology; D014357:Trypsin; D014776:Virus Cultivation / Q000379:methods*; D014779:Virus Replication
D000956:Antigens, Viral; D006389:Hemagglutinins, Viral; D007252:Influenza Vaccines; D004492:Edetic Acid; D014357:Trypsin