Flow cytometric detection of host cell apoptosis induced by bacterial infection.
We detail two methods for detection of cell death induced by infection of a human monocytic cell line with invasive Campylobacter bacteria. Staining with a natural ligand for exposed phosphatidylserine residues coupled with propidum iodide discriminated between apoptosis and necrosis. Additionally, cells infected with a bacterial strain expressing green fluorescent protein stained with dye sensitive to mitochondrial membrane potential demonstrated a direct association of bacteria with dying cells. Analyses of cells stained by these methods employing flow cytometry enumerated proportions of cell populations undergoing either apoptosis or necrosis after bacterial infection in vitro.
Konkel
M E
ME
Program in Microbiology, School of Molecular Biosciences, Washington State University, Pullman, WA 99164-4233 USA.
Mixter
P F
PF
D017209:Apoptosis; D002169:Campylobacter Infections / Q000382:microbiology*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D066298:In Vitro Techniques; D009000:Monocytes / Q000382:microbiology*; D009336:Necrosis; D013194:Staining and Labeling