Exploiting molecular mimicry to broaden the immune response to carbohydrate antigens for vaccine development.
Peptide mimetics of carbohydrates represent an alternative approach to induce anti-carbohydrate responses. Depending on their formulation, peptide mimetics can mediate T-independent or T-dependent responses. Multivalent peptide mimeotopes can induce high IgM/IgG ratios, as non-conjugated carbohydrates do. Here we observe that immunization with multivalent peptide mimeotope conjugated to BSA enhances carbohydrate reactive antibodies in Balb/c mice and xid mice, with IgG1 greater than IgG2a, in xid mice. These results suggest that mimeotope-conjugate formulations might augment carbohydrate-specific immune responses in immuno-compromised hosts.
Department of Pathology and Laboratory Medicine, Philadelphia, PA 19104-6082, USA.
G
G
Cunto-Amesty
P
P
Luo
B
B
Monzavi-Karbassi
A
A
Lees
T
T
Kieber-Emmons
D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000737:chemistry*; D001402:B-Lymphocytes / Q000276:immunology; D002241:Carbohydrates / Q000737:chemistry / Q000276:immunology*; D003429:Cross Reactions; D005260:Female; D007114:Immunization; D007983:Lewis Blood Group Antigens / Q000737:chemistry / Q000235:genetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D008819:Mice, Nude; D008954:Models, Biological; D018716:Molecular Mimicry; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014612:Vaccines / Q000276:immunology*; D018074:Vaccines, Conjugate / Q000494:pharmacology
D000941:Antigens; D002241:Carbohydrates; D007983:Lewis Blood Group Antigens; C094162:Lewis Y antigen; D010455:Peptides; D014612:Vaccines; D018074:Vaccines, Conjugate