Continuous determination of chloroquine in plasma by laser-induced photochemical reaction and fluorescence.
A flow injection fluorimetric method is proposed for the determination of chloroquine based on the photochemical derivatisation in an alkaline medium of the analyte and fluorescence generation after irradiation with a pulsed Nd:YAG laser operated at 355 nm. Chemical, hydrodynamic and laser variables were studied in order to obtain the best conditions for quantification. A linear range from 25 to 600 micrograms/L was achieved, with a correlation coefficient of 0.997 (n = 8), an RSD of 4.3% (n = 11) and a detection limit of 8 micrograms/L (3 sigma). The sample throughput was 10 h-1. The method was successfully applied to the determination of chloroquine in human plasma. The increase of sensitivity with respect to the method based on monitoring the intrinsic fluorescence of chloroquine itself was 1.7 times.
Analytical Chemistry Division, Faculty of Sciences, University of Córdoba, 14004-Córdoba, Spain.
J
J
Amador-Hernández
J M
JM
Fernández-Romero
M D
MD
Luque de Castro
D002738:Chloroquine / Q000032:analysis* / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D007834:Lasers; D010949:Plasma / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D002738:Chloroquine