Tsukamurella strandjordae sp. nov., a proposed new species causing sepsis.
We have isolated a gram-positive, weakly acid-alcohol-fast, irregular rod-shaped bacterium from cultures of blood from a 5-year-old girl with acute myelogenous leukemia. This isolate was compared with 14 other strains including reference strains of Tsukamurella species by a polyphasic approach based on physiological and biochemical properties, whole-cell short-chain fatty acid and mycolic acid analyses, DNA-DNA hybridization, and sequencing of the 16S rRNA gene. This isolate represents a new taxon within the genus Tsukamurella for which we propose the name Tsukamurella strandjordae sp. nov. Our study also revealed that Tsukamurella paurometabola ATCC 25938 represents a misnamed Tsukamurella inchonensis isolate and confirms that Tsukamurella wratislaviensis belongs to the genus Rhodococcus.
Kattar
M M
MM
Department of Laboratory Medicine, Division of Infectious Disease, University of Washington, Seattle, Washington, USA.
Cookson
B T
BT
Carlson
L D
LD
Stiglich
S K
SK
Schwartz
M A
MA
Nguyen
T T
TT
Daza
R
R
Wallis
C K
CK
Yarfitz
S L
SL
Coyle
M B
MB
D000192:Actinomycetales / Q000737:chemistry / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification / Q000502:physiology; D000193:Actinomycetales Infections / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D002675:Child, Preschool; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D020459:Genes, rRNA; D006801:Humans; D008969:Molecular Sequence Data; D009171:Mycolic Acids / Q000032:analysis; D009693:Nucleic Acid Hybridization; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012015:Reference Standards; D018805:Sepsis / Q000382:microbiology*; D017422:Sequence Analysis, DNA
D005227:Fatty Acids; D009171:Mycolic Acids; D012336:RNA, Ribosomal, 16S