Inhibitory effects of 8-(N,N-diethylamino)-n-octyl-3,4,5-trimethoxybenzoate (TMB-8) on intracellular Ca2+ elevated by neurotransmitters in brain cells.
OBJECTIVE
To study the effects of TMB-8 on [Ca2+]i elevation induced by neurotransmitters in dissociated brain cells.


METHODS
The brain cell suspension was made using a gentle trituration for 1 min with a polished pipette. The changes of [Ca2+]i were detected by the fluorescent indicator, Fura 2-AM.


RESULTS
In the presence of extracellular Ca2+ 1.3 mmol.L-1, sodium glutamate (Glu), histamine (His), and serotonin (5-HT) markedly increased the [Ca2+]i which were reduced by TMB-8 30 mumol.L-1. TMB-8 3 mumol.L-1 produced inhibitory effects on the increase of [Ca2+]i by His and 5-HT in a Ca(2+)-free Hanks' solution. The increase of [Ca2+]i by His and 5-HT was reduced to control level by TMB-8 10 mumol.L-1.


CONCLUSIONS
TMB-8 inhibited the [Ca2+]i elevation induced by Glu, 5-HT, and His in brain cells.
Department of Pharmacology, Nanjing Medical University, Nanjing 210029, China.
X Q
XQ
Zhang
B
B
Wang
M Y
MY
Zhang
J G
JG
Xiao
D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002478:Cells, Cultured; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology*; D006632:Histamine; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin; D012970:Sodium Glutamate / Q000037:antagonists & inhibitors
D002121:Calcium Channel Blockers; D012701:Serotonin; C006014:8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate; D005707:Gallic Acid; D006632:Histamine; D002118:Calcium; D012970:Sodium Glutamate