Intrahepatic metastasis by orthotopic implantation of a fragment of murine hepatoma and its related molecules.
Intrahepatic metastasis is a major modality in the recurrence of hepatoma. Establishment of the intrahepatic metastasis model would be useful for evaluating new anticancer therapies and analyzing the molecular mechanisms of tumor metastasis. Orthotopic implantation of a fragment of CBO140C12 hepatoma into the liver resulted in the formation of a solitary tumor nodule and its intrahepatic metastasis. In contrast, implantation of ADras3 cancer cells did not show any metastasis on day 21. CBO140C12 cells showed enhancement of the invasive, adhesive and migratory capabilities, as compared with ADras3 cells. Furthermore, mRNA expression and gelatinolytic activity of MMP-9 were detected in CBO140C12 cells, and the expression of mRNA for MT1-MMP in CBO140C12 cells was greater than that in ADras3 cells. Thus, intrahepatic metastasis of CBO140C12 tumor might be involved in the enhancement of the invasiveness of tumor cells via marked expression of MMP-9 and MT1-MMP.
Department of Pathogenic Biochemistry, Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, Toyama, Japan.
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S
Sawada
K
K
Murakami
T
T
Yamaura
T
T
Sakamoto
K
K
Ogawa
K
K
Tsukada
I
I
Saiki
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D018093:Gelatinases / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000473:pathology* / Q000556:secondary; D053511:Matrix Metalloproteinase 14; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D053504:Matrix Metalloproteinases, Membrane-Associated; D008666:Metalloendopeptidases / Q000378:metabolism; D051379:Mice; D008809:Mice, Inbred C3H; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D012333:RNA, Messenger / Q000378:metabolism; D014407:Tumor Cells, Cultured
C507096:Mmp14 protein, mouse; D012333:RNA, Messenger; D018093:Gelatinases; D053504:Matrix Metalloproteinases, Membrane-Associated; D008666:Metalloendopeptidases; D020780:Matrix Metalloproteinase 9; D053511:Matrix Metalloproteinase 14