N-isobutyloxycarbonylation for improved detection of 3'-hydroxystanozolol and its 17-epimer in doping control.
An improved screening method was developed for 3'-hydroxystanozolol and its 17-epimer in human urine involving gas chromatography-mass spectrometry (GC-MS) with N-isobutyloxycarbonyl (isoBOC) and O-trimethylsilyl (TMS) derivatization. A procedure was reported previously for the pentane extraction of many steroids from urine in doping control, but it was not suitable for the detection of stanozolol metabolites. Compared with the n-pentane extraction method, which gave a poor recovery (< 10%), isoBOC extraction resulted in a good recovery (> 80%). The sensitivity and specificity of mixed N-isoBOC-O-TMS derivatization were adequate for the detection of 3'-hydroxystanozolol and its 17-epimer when 3 ml of urine was used with spiking at a level of 2 ng ml-1. When applied to a stanozolol-positive urine sample, the proposed method allowed rapid and sensitive screening for the detection of 3'-hydroxystanozolol and its 17-epimer.
Choi
M H
MH
Bioanalysis and Biotransformation Research Center, KIST, P.O. Box 131, Cheongryang, Seoul 130-650, Korea.
Chung
B C
BC
D045930:Anabolic Agents; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D013197:Stanozolol / Q000031:analogs & derivatives / Q000652:urine; D015813:Substance Abuse Detection / Q000379:methods*
C425490:3'-hydroxy-17-epistanozolol; D045930:Anabolic Agents; C425489:3'-hydroxystanozolol; D013197:Stanozolol