Structure and expression of a CTP: phosphocholine cytidylyltransferase gene from Arabidopsis thaliana.
A genomic clone, which includes the CTP: phosphocholine cytidylyltransferase (CCT) open reading frame and its 5'- and 3'- flanking non-coding regions, has been isolated from Arabidopsis thaliana and sequenced. The CCT gene is approximately 3.0 kb in length and contains 8 exons interrupted by 7 introns, which range from 74 to 626 nucleotides. All nucleotide sequences for the intron 3' splice sites are consistent with the consensus AG sequence of plant pre-mRNA processing, while the major GT consensus sequence for the 5' splice site is conserved in 5 of 7 introns. Introns 5 and 6 have a minor GC consensus sequence instead. In the 5'-flanking region there are two sequences related to a cold-responsive element found in the cold-inducible promoter of the A. thaliana cor15a gene, plus one gibberellin-response element. The results from reverse transcriptase-PCR indicate that the expression of A. thaliana CCT is regulated by temperature. The expression level of CCT increased after a 30-min treatment at 5 degrees C. When the plants were returned to 22 degrees C, the expression of CCT also decreased to the original level.
Department of Biology and Institute of Molecular Cell Biology, Inha University, Inchon, Korea.
Y H
YH
Choi
J K
JK
Lee
S H
SH
Cho
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics*; D001483:Base Sequence; D019916:Choline-Phosphate Cytidylyltransferase / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D003080:Cold Temperature; D016384:Consensus Sequence; D005091:Exons; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D007438:Introns; D008969:Molecular Sequence Data; D010713:Phosphatidylcholines / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D012322:RNA Precursors / Q000235:genetics; D022821:RNA Splice Sites / Q000235:genetics
D010713:Phosphatidylcholines; D010940:Plant Proteins; D012322:RNA Precursors; D022821:RNA Splice Sites; D019916:Choline-Phosphate Cytidylyltransferase