UCP3 expressed in yeast is primarily localized in extramitochondrial particles.
Previously it was concluded (1) that, differently from UCP1, on expression in Saccharomyces cerevisiae, UCP3, and UCP3 short (UCP3s) are in a deranged state, allowing for unregulated uncoupling. Here we show that the bulk of UCP3 and UCP3s is in extramitochondrial aggregates whether expressed with high or medium expression vectors. The evidence is based on the insolubility of most UCP3 and UCP3s in nonionic detergents such as Triton X100, in contrast to UCP1. Using very high expression vector, macroscopic evidence for extramitochondrial UCP3 containing particles is a viscous white sediment surrounding the mitochondrial fraction which contains UCP3 as inclusion body type aggregate. Together with the previous data it is concluded that uncoupling due to small amounts of incorporated, deranged, and nucleotide insensitive UCP3 prevents incorporation of the bulk of UCP3 into mitochondria. This finding also provides a simple and stringent assay for the state of heterologously expressed in mitochondrial membrane proteins.
Winkler
E
E
Institute for Physical Biochemistry, University of Munich, Schillerstrasse 44, Munich, 80336, Germany.
Heidkaemper
D
D
Klingenberg
M
M
Liu
Q
Q
Caskey
T
T
D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D007473:Ion Channels; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013347:Subcellular Fractions / Q000378:metabolism*; D000071247:Uncoupling Protein 3
D002352:Carrier Proteins; D007473:Ion Channels; D024101:Mitochondrial Proteins; D000071247:Uncoupling Protein 3