DnaA protein dependent denaturation of negative supercoiled oriC DNA minicircles.
The DnaA protein binds specifically and tightly to oriC supercoiled 641 bp minicircle DNA. The binding of the initiator promoted a partial unwinding of the superhelical oriC minicircle (Mc-oriC). Open complexes are detected either by a change in the linking number or by the sensitivity to the attack of a single strand specific Bal 31 nuclease. The open complex is found only in the presence of the DnaA protein.
Landoulsi
A
A
Département de Biologie, Laboratoire de Biochimie des Proteines, Faculté des Sciences de Tunis, Campus Universitaire, 1060, Tunis, Tunisia.
Kohiyama
M
M
D000255:Adenosine Triphosphate / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004264:DNA Topoisomerases, Type I / Q000235:genetics / Q000378:metabolism*; D004278:DNA, Superhelical / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D018741:Replication Origin / Q000235:genetics*
D001426:Bacterial Proteins; D004278:DNA, Superhelical; D004268:DNA-Binding Proteins; C066312:DnaA protein, Bacteria; D000255:Adenosine Triphosphate; D004264:DNA Topoisomerases, Type I