Inhibition of a glucose-limited sequencing fed-batch culture of Salmonella enterica serovar enteritidis by volatile fatty acids representative of the ceca of broiler chickens.
The effects of concentrations of volatile fatty acids on an anaerobic, glucose-limited, and pH-controlled growing culture of Salmonella enterica serovar Enteritidis were studied. Suddenly increasing volatile fatty acids to the concentrations representative of the ceca of 15-day-old broiler chickens caused washout of serovar Enteritidis. In contrast, a sudden increase to the volatile fatty acid concentrations representative of the ceca of younger broiler chickens caused a reduction in the biomass but not washout. Gradually increasing volatile fatty acids caused a gradual decrease in the biomass of serovar Enteritidis. We conclude that the concentrations of volatile fatty acids present in the ceca of broilers with a mature microflora can cause washout of serovar Enteritidis in an in vitro system mimicking cecal ecophysiology.
Centre for Veterinary Public Health and Environmental Protection, Faculty of Veterinary Medicine, Utrecht University, P.O. Box 80175, 3508 TD Utrecht, The Netherlands. wielen@vvdo.vet.uu.nl
P W
PW
van der Wielen
S
S
Biesterveld
L J
LJ
Lipman
F
F
van Knapen
D000693:Anaerobiosis; D000818:Animals; D002432:Cecum / Q000737:chemistry*; D002645:Chickens; D003470:Culture Media; D005232:Fatty Acids, Volatile / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000378:metabolism; D012477:Salmonella enteritidis / Q000187:drug effects* / Q000254:growth & development*
D003470:Culture Media; D005232:Fatty Acids, Volatile; D007773:Lactates; D005947:Glucose