A new family of small, palmitoylated, membrane-associated proteins, characterized by the presence of a cysteine-rich hydrophobic motif.
We recently cloned the CHIC2 gene (previously BTL) by virtue of its involvement in a chromosomal translocation t(4;12)(q11;p13) occurring in acute myeloid leukemias. In this study we show that CHIC2 is a member of a highly conserved family of proteins characterized by the presence of a striking cysteine-rich hydrophobic (CHIC) motif. Our data illustrate that cysteines in this central CHIC motif are palmitoylated and that CHIC2 is associated with vesicular structures and the plasma membrane. The CHIC proteins thus resemble the cysteine string proteins, which function in regulated exocytosis.
The Human Genome Laboratory, Center for Human Genetics, University of Leuven, Flanders Interuniversity Institute for Biotechnology (VIB), Herestraat 49, B-3000, Leuven, Belgium.
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Cools
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Mentens
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Marynen
D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D003545:Cysteine / Q000737:chemistry* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D049452:Green Fluorescent Proteins; D006801:Humans; D020014:K562 Cells; D008164:Luminescent Proteins / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry* / Q000145:classification / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D051381:Rats; D017386:Sequence Homology, Amino Acid; D013347:Subcellular Fractions; D014157:Transcription Factors / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D022161:Transport Vesicles / Q000378:metabolism
C121401:CHIC2 protein, human; D004268:DNA-Binding Proteins; D008164:Luminescent Proteins; D008565:Membrane Proteins; D016271:Proto-Oncogene Proteins c-myc; D014157:Transcription Factors; D049452:Green Fluorescent Proteins; D003545:Cysteine