beta-Conglycinin and glycinin in high-protein soybean seeds.
The agronomic performance and storage proteins of high seed protein lines of soybeans [Glycine max L. (Merr.)] were investigated to determine if the two major storage proteins, beta-conglycinin and glycinin, contribute to the increased protein content of high seed protein lines. Subunits of these two major storage proteins were estimated by scanning SDS-PAGE gels by scanning densitometry. The relative rankings of the lines with respect to seed size and protein content were not different between years in one environment over 5 years, but oil and total protein and oil contents and the ratio of protein to oil differed. The alpha', alpha, and beta subunits of beta-conglycinin were significantly higher in the high-protein lines except CX797-115, CX804-108, CX804-3, D81-8498, and NC-2-62. The acidic A(3) polypeptide of glycinin was significantly higher in high-protein lines except 76-48773, CX804-108, CX804-3, D81-8498, and NC-2-62, whereas the acidic polypeptides A(1,2,4) of glycinin were significantly higher in all of the high-protein lines. The basic polypeptides of glycinin were significantly higher in all high seed protein lines except D81-8259. In conclusion, high-protein lines appear to contain more beta-conglycinin and glycinin than normal-protein soybean lines, and the amounts of subunits and polypeptides differ among lines.
Yaklich
R W
RW
Soybean and Alfalfa Research Laboratory, Building 006, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705, USA.
D052179:Antigens, Plant; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005916:Globulins / Q000032:analysis*; D010940:Plant Proteins / Q000032:analysis; D021122:Protein Subunits; D055314:Seed Storage Proteins; D012639:Seeds / Q000737:chemistry*; D013024:Soybean Oil / Q000737:chemistry; D030262:Soybean Proteins; D013025:Glycine max / Q000737:chemistry*; D013045:Species Specificity
D052179:Antigens, Plant; D005916:Globulins; D010940:Plant Proteins; D021122:Protein Subunits; D055314:Seed Storage Proteins; D030262:Soybean Proteins; C013894:beta-conglycinin protein, Glycine max; D013024:Soybean Oil; C011474:glycinin