Helicobacter pylori genotypes, host factors, and gastric mucosal histopathology in peptic ulcer disease.
From 183 patients undergoing upper gastrointestinal endoscopy, we used antral and corpus gastric biopsies for bacterial culture and histopathologic examination, blood samples to detect immunoglobulin G antibodies against Helicobacter pylori, and H pylori genomic DNA to analyze cytotoxin-associated gene A (cagA) and vacuolating cytotoxin (vacA) genotypes. As expected, among H pylori biopsy-positive patients, those with duodenal ulcer (DU) (n = 34) had significantly more severe chronic and acute inflammation (P <.001) and epithelial degeneration (P =.004) in the gastric antrum than in the gastric corpus. Each of those 3 parameters and H pylori density were significantly higher in the antrum of patients with DU than in patients with gastric ulcer (GU) or no ulcer. Colonization with vacA s1/cagA-positive strains of H pylori was associated with inflammation and epithelial degeneration in gastric mucosa and increased risk for peptic ulcer disease (PUD), whereas colonization with vacA s2m2/cagA-negative strains was associated with mild gastric histopathology and was not associated with any significant risk for PUD. The predominant H pylori strains in African Americans were vacA s1bm1/cagA-positive, whereas all genotypes were well represented in non-Hispanic-Caucasians. By multivariate analysis, H pylori colonization was significantly associated with DU (Adjusted odds ratio [AdjOR] = 3.2 [1.4-7.2]) and nonsteroidal anti-inflammatory drugs (NSAID) use was inversely associated (AdjOR = 0.3 [0.2-0.7]). NSAID use (AdjOR = 4.3 [1.02-18.5]) and African-American ethnicity (AdjOR = 10.9 [2.6-50]) were significantly associated with GU. Smoking and age were not significantly associated with either DU or GU. These data indicate that DU is associated with an antral-dominant gastritis, and H pylori genotype and NSAID use independently contribute to the pathogenesis of PUD. HUM PATHOL 32:264-273. This is a US Government work. There are no restrictions on its use.
Tham
K T
KT
Department of Pathology, Vanderbilt University School of Medicine, Nashville, TN, USA.
Peek
R M
RM
Atherton
J C
JC
Cover
T L
TL
Perez-Perez
G I
GI
Shyr
Y
Y
Blaser
M J
MJ
D000368:Aged; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use; D000907:Antibodies, Bacterial / Q000097:blood; D001706:Biopsy; D044383:Black People; D004381:Duodenal Ulcer / Q000175:diagnosis / Q000382:microbiology / Q000473:pathology; D004848:Epithelium / Q000473:pathology; D005006:Ethnicity; D005753:Gastric Mucosa / Q000473:pathology*; D005838:Genotype; D016481:Helicobacter Infections / Q000175:diagnosis / Q000382:microbiology* / Q000473:pathology*; D016480:Helicobacter pylori / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D008875:Middle Aged; D015999:Multivariate Analysis; D010437:Peptic Ulcer / Q000175:diagnosis / Q000382:microbiology* / Q000473:pathology; D011706:Pyloric Antrum / Q000382:microbiology / Q000473:pathology; D012907:Smoking; D013270:Stomach / Q000382:microbiology / Q000473:pathology; D013276:Stomach Ulcer / Q000175:diagnosis / Q000382:microbiology / Q000473:pathology; D044465:White People; D001741:Black or African American
D000894:Anti-Inflammatory Agents, Non-Steroidal; D000907:Antibodies, Bacterial