[Formation of C/EBP transcription factors and possible ways of regulation of their activity during learning in Helix].
Defensive conditioning in Helix is associated with activation of C/EBP family of transcription factors. The incubation of the CNS with serotonin that imitates the effects of conditioning or stimulation of adenylate cyclase with forscolin also increase the DNA-binding activity of C/EBP. The induction of protein kinase C by phorbol ester does not stimulate the formation of these transcription complexes. However, a slight increase in the cAMP-induced influence on the activation of C/EBP transcription factors was observed during a combined action of phorbol ester and serotonin. The more substantial cAMP-induced rise in DNA-binding activity of C/EBP family transcription factors was observed during increase in the intracellular Ca2+ concentration (incubation of the CNS with calcium ionophor A23187 and forscolin). Thus, the convergence of Ca2+ and cAMP-dependent regulating signals that reflect the interaction of different modal stimuli during learning can produce the intensification of formation of the C/EBP transcription complexes.
Pavlov Institute of Physiology, Russian Academy of Sciences, St.-Petersburg.
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Grinkevich
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D001362:Avoidance Learning; D022762:CCAAT-Enhancer-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D005576:Colforsin / Q000494:pharmacology; D004789:Enzyme Activation; D004790:Enzyme Induction; D017952:Ganglia, Invertebrate / Q000378:metabolism; D005786:Gene Expression Regulation; D006372:Helix, Snails; D066298:In Vitro Techniques; D007476:Ionophores / Q000494:pharmacology; D009841:Oligonucleotides; D011493:Protein Kinase C / Q000096:biosynthesis; D012701:Serotonin / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D022762:CCAAT-Enhancer-Binding Proteins; D007476:Ionophores; D009841:Oligonucleotides; D005576:Colforsin; D012701:Serotonin; D000001:Calcimycin; D011493:Protein Kinase C; D000262:Adenylyl Cyclases; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium