Monoamine release by neurons of a primitive nervous system: an amperometric study.
We measured monoamine release from dissociated neurons of the sea pansy Renilla koellikeri, a representative of the most evolutionarily ancient animals with nervous systems, by real-time monitoring of exocytosis using the amperometric method with carbon-fiber microelectrodes. Depolarization-induced, as well as spontaneously active, neurons exhibited calcium-dependent exocytotic events at both the soma and the terminal bulb of neuritic processes. All spontaneously active neurons exhibited a bursting activity pattern in which amplitudes of exocytotic events appeared to be distributed in a quantal-like fashion. Fast Fourier transform analysis of bursting activity in 20 such neurons revealed burst harmonics with a major frequency of 8 Hz and a dominant rate of 95 Hz for individual exocytotic events within bursts. The results suggest that exocytotic transmitter release is as ancient as neurons and that endogenously bursting neurons in the sea pansy are as complex as those of higher animals. In addition, the observation that both soma and neuritic terminals of the same neuron can release transmitter suggests that local release sites in these cnidarian neurons are not critical for nerve net function.
Département de sciences biologiques and Centre de recherches en sciences neurologiques, Université de Montréal, Montréal, Canada.
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D000818:Animals; D015306:Biogenic Monoamines / Q000378:metabolism*; D002478:Cells, Cultured; D003063:Cnidaria / Q000502:physiology*; D004594:Electrophysiology / Q000379:methods; D005089:Exocytosis / Q000187:drug effects; D005583:Fourier Analysis; D015759:Ionomycin / Q000494:pharmacology; D007700:Kinetics; D008163:Luminescent Measurements; D008564:Membrane Potentials / Q000187:drug effects; D008839:Microelectrodes; D009424:Nervous System Physiological Phenomena; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009479:Neuropeptides / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D014701:Veratridine / Q000494:pharmacology
D015306:Biogenic Monoamines; D009479:Neuropeptides; C060288:4-pyroglutamyl-glycyl-arginyl-phenylalaninamide; D015759:Ionomycin; D011189:Potassium Chloride; D014701:Veratridine; D011761:Pyrrolidonecarboxylic Acid