The use of Pseudomonas acyl-CoA synthetase to form acyl-CoAs from dicarboxylic fatty acids.
Pseudomonas acyl-CoA synthetase is shown to act on saturated dicarboxylic acids with a chain length of C10 or greater to produce conjugates containing a single CoA unit. The synthetase can, therefore, be used to generate novel acyl-CoA analogues for studies on proteins that utilise, bind to, or are modulated by acyl-CoAs.
Division of Biological Chemistry and Molecular Parasitology, The Wellcome Trust Biocentre, University of Dundee, DD1 5EH, Dundee, UK.
K G
KG
Milne
A
A
Mehlert
M A
MA
Ferguson
D000214:Acyl Coenzyme A / Q000138:chemical synthesis; D003066:Coenzyme A Ligases / Q000737:chemistry*; D003998:Dicarboxylic Acids / Q000737:chemistry*; D005227:Fatty Acids / Q000737:chemistry*; D011549:Pseudomonas / Q000201:enzymology*; D013045:Species Specificity
D000214:Acyl Coenzyme A; D003998:Dicarboxylic Acids; D005227:Fatty Acids; D003066:Coenzyme A Ligases