Morphological correlates of functionally defined synaptic vesicle populations.
By combining photoconversion of FM1-43-stained vesicles and electron microscopy of hippocampal synapses, we find evidence that the population of morphologically docked synaptic vesicles corresponds to the release-ready neurotransmitter quanta. Furthermore, those synaptic vesicles that are participating in cycles of exo- and endocytosis tend to be closer to the active zone than vesicles that are being held in reserve.
Schikorski
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Howard Hughes Medical Institute and Molecular Neurobiology Lab, The Salk Institute, 10010 N. Torrey Pines Road, La Jolla, California 92037, USA.
Stevens
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CF
D000818:Animals; D002478:Cells, Cultured; D005456:Fluorescent Dyes / Q000032:analysis; D006624:Hippocampus / Q000737:chemistry / Q000166:cytology* / Q000648:ultrastructure; D007091:Image Processing, Computer-Assisted; D009474:Neurons / Q000737:chemistry* / Q000648:ultrastructure; D018377:Neurotransmitter Agents / Q000032:analysis*; D011726:Pyridinium Compounds / Q000032:analysis; D000644:Quaternary Ammonium Compounds / Q000032:analysis; D051381:Rats; D012044:Regression Analysis; D013569:Synapses / Q000648:ultrastructure; D013572:Synaptic Vesicles / Q000737:chemistry* / Q000648:ultrastructure*
C073804:FM1 43; D005456:Fluorescent Dyes; D018377:Neurotransmitter Agents; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds