Characterisation of stage-specific proteins of Oesophagostomum dentatum by preparative isoelectric focusing and lectin blotting.
The nodular worm of pigs, Oesophagostomum dentatum, has previously been shown to undergo distinct biochemical changes during its life cycle. This phenomenon was studied in more detail for the early parasitic stages. Differences between infective third-stage larvae (L3), parasitic fourth-stage larvae cultivated in vitro (L4c), and pre-adult larvae recovered from the intestinal contents of pigs (L4p) were compared with respect to their protein and glycoprotein patterns by solubility-based protein fractionation and preparative isoelectric focusing followed by SDS-PAGE or by Western blotting with various lectins. While differences between the L4 were only minor (only three bands were specific for either L4c or L4p), L3 displayed distinctly different protein patterns with four L3-specific and nine L4-specific bands. Concanavalin A bound to a variety of glycoproteins, partly in a stage-specific manner, while Ricinus communis Agglutinin 120, Wheat Germ Agglutinin, Peanut Agglutinin and Soybean Agglutinin bound to fewer, partly stage-specific, molecules.
Institut für Parasitologie, Tierärztliche Hochschule Hannover, Bünteweg 17, D-30559, Hannover, Germany. baerbel.ruttkowski@tiho-hannover.de
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Joachim
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Ruttkowski
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Daugschies
D000818:Animals; D015153:Blotting, Western / Q000379:methods; D015801:Helminth Proteins / Q000032:analysis* / Q000378:metabolism; D007525:Isoelectric Focusing; D037102:Lectins / Q000378:metabolism*; D009814:Oesophagostomiasis / Q000662:veterinary; D009815:Oesophagostomum / Q000737:chemistry* / Q000254:growth & development*; D013552:Swine; D013553:Swine Diseases / Q000469:parasitology
D015801:Helminth Proteins; D037102:Lectins