Complete sequences and phylogenetic analyses of a Singapore isolate of broad bean wilt fabavirus.
The complete nucleotide (nt) sequence of a Singapore isolate of broad bean wilt fabavirus from Megakepasma erythrochlamys L., designated BBWV-ME, was determined. Its bipartite genome consisted of two positive-sense single-stranded ribonucleic acids (RNA). RNA1 (5951 nt in length) encoded a putative protease cofactor, nucleotide triphosphate (NTP)-binding domain (helicase), viral genome-linked protein (VPg), protease and RNA-dependent RNA polymerase (RdRp). RNA2 (3607 nt in length) encoded a putative movement protein (MP) and coat proteins (CP). Genome organization of BBWV-ME was similar to other viruses in the Comoviridae family. Phylogenetic analyses showed that fabaviruses were more closely related to the comoviruses than the nepoviruses.
Department of Biological Sciences, The National University of Singapore.
L H
LH
Koh
J I
JI
Cooper
S M
SM
Wong
D001483:Base Sequence; D002213:Capsid / Q000235:genetics; D003001:Cloning, Molecular; D010450:Endopeptidases / Q000235:genetics; D007887:Fabaceae / Q000821:virology*; D019181:Fabavirus / Q000145:classification / Q000235:genetics*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D053830:Plant Viral Movement Proteins; D010946:Plants, Medicinal; D020365:RNA Helicases / Q000235:genetics; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012846:Singapore; D014764:Viral Proteins / Q000235:genetics
D053830:Plant Viral Movement Proteins; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase; D010450:Endopeptidases; D020365:RNA Helicases