[Noninvasive screening for genital chlamydiosis in adolescents in Brno].
OBJECTIVE
To run a screening for genital chlamydiosis in adolescents living in the town Brno as the first action of this type in the Czech Republic and to use the results of the screening for the elaboration of recommendations for running similar actions on the national scale.


METHODS
Prospective epidemiological study.


METHODS
Veterinary Research Institute, Brno; Institute of Clinical Biochemistry, 1st Faculty of Medicine, Charles University and General Faculty Hospital, Prague; Section of Public Health, Municipal Authorities of Brno; Bioplus Ltd., Brno; Regional Hygienic Services, Brno; Faculty Hospital Brno; with technical assistance of teachers and students of two Medical Assistant Schools in Brno.


METHODS
Students (337 females and 15 males) of two Medical Assistant Schools, older than 18 years, were used as probands within the study. Sediments of the first portions of urine collected from the individual probands were tested for the presence of Chlamydia trachomatis using the direct fluorescent test, ELISA, and the ligase chain reaction.


RESULTS
Positive reactions in any of the three tests were found in 31 of the 352 probands (8.8%). Positive and doubtful reactions in the direct fluorescent antibody test were obtained in 11 (35.5%) and 3 (9.6%) of the 31 reactors, respectively. The corresponding values for ELISA were 9 (23.0%) and 8 (25.8%), respectively, and those for the ligase chain reaction 3 (16.6%) and 3 (16.6%), respectively. The overall prevalence of 8.8% is higher than the European mean.


CONCLUSIONS
The first limited screening for genital Chlamydia infections in the Czech Republic was run in Brno. Urinary samples were collected from 337 females and 15 males aged approximately 18 years. The presence of Chlamydia trachomatis in the urinary sediment was demonstrated by the direct fluorescent test, ELISA, and the ligase chain reaction. The established prevalence of 8.8% exceeded the European mean (3.9%).
Výzkumný ústav veterinárního lékarství Brno.
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Pospísil
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Bucek
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Diblíková
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Drazd'áková
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Medková
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Pejcoch
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Prosková
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Unzeitig
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Vĕzník
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Zralý
D000293:Adolescent; D002690:Chlamydia Infections / Q000175:diagnosis* / Q000453:epidemiology; D002692:Chlamydia trachomatis; D018153:Czech Republic / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005831:Genital Diseases, Female / Q000175:diagnosis / Q000453:epidemiology; D005832:Genital Diseases, Male / Q000175:diagnosis / Q000453:epidemiology; D006801:Humans; D008297:Male; D008403:Mass Screening; D015995:Prevalence; D015231:Sexually Transmitted Diseases, Bacterial / Q000175:diagnosis* / Q000453:epidemiology