Inhibition of a glucose-limited sequencing fed-batch culture of Salmonella enterica serovar enteritidis by volatile fatty acids representative of the ceca of broiler chickens.
The effects of concentrations of volatile fatty acids on an anaerobic, glucose-limited, and pH-controlled growing culture of Salmonella enterica serovar Enteritidis were studied. Suddenly increasing volatile fatty acids to the concentrations representative of the ceca of 15-day-old broiler chickens caused washout of serovar Enteritidis. In contrast, a sudden increase to the volatile fatty acid concentrations representative of the ceca of younger broiler chickens caused a reduction in the biomass but not washout. Gradually increasing volatile fatty acids caused a gradual decrease in the biomass of serovar Enteritidis. We conclude that the concentrations of volatile fatty acids present in the ceca of broilers with a mature microflora can cause washout of serovar Enteritidis in an in vitro system mimicking cecal ecophysiology.
van der Wielen
P W
PW
Centre for Veterinary Public Health and Environmental Protection, Faculty of Veterinary Medicine, Utrecht University, P.O. Box 80175, 3508 TD Utrecht, The Netherlands. wielen@vvdo.vet.uu.nl
Biesterveld
S
S
Lipman
L J
LJ
van Knapen
F
F
D000693:Anaerobiosis; D000818:Animals; D002432:Cecum / Q000737:chemistry*; D002645:Chickens; D003470:Culture Media; D005232:Fatty Acids, Volatile / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000378:metabolism; D012477:Salmonella enteritidis / Q000187:drug effects* / Q000254:growth & development*
D003470:Culture Media; D005232:Fatty Acids, Volatile; D007773:Lactates; D005947:Glucose