Detection of cell membrane proteins that interact with virulent infectious bursal disease virus.
To detect the molecules that interact with infectious bursal disease virus (IBDV), the chicken B lymphoblastoid cell line, LSCC-BK3, which is permissive for virulent IBDV infection was investigated. The sodium dodecyl sulfate-solubilized plasma membrane fraction from the cells was subjected to a virus overlay protein binding assay. The IBDV specifically bound to proteins in LSCC-BK3 plasma membranes with molecular weights of 70, 82 and 110 kDa. This is the first report to demonstrate cellular molecules that interact with virulent IBDV.
Setiyono
A
A
Department of Veterinary Microbiology, Faculty of Agriculture, Gifu University, Japan.
Hayashi
T
T
Yamaguchi
T
T
Fukushi
H
H
Hirai
K
K
D000818:Animals; D018175:Birnaviridae Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D002462:Cell Membrane / Q000821:virology; D002478:Cells, Cultured; D002645:Chickens; D007243:Infectious bursal disease virus / Q000276:immunology / Q000378:metabolism / Q000472:pathogenicity*; D008565:Membrane Proteins / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D008970:Molecular Weight; D011201:Poultry Diseases / Q000276:immunology / Q000821:virology*; D011485:Protein Binding / Q000276:immunology
D008565:Membrane Proteins