Quantification of carbohydrate structures in size fractionated aquatic humic substances by two-dimensional nuclear magnetic resonance.
Two-dimensional phase sensitive C,H correlation spectra were successfully applied to the quantification of carbohydrate substructures in aquatic humic substance (HS) fractions obtained by tangential flow multistage ultrafiltration (TFMSTUF) of a selected bog water HS (HO13, German Research Program DFG-ROSIG) as well as a river HS (Suwannee River Fulvic Acid Reference of the International Humic Substances Society, IHSS). It turns out that after size fractionation the HS samples give very well resolved C,H-correlation spectra which offer a great potential for substructure quantification. Details of the combined substructure quantification technique, novel in HS characterization, are presented. The results of the combined procedure point out that carbohydrate moieties predominantly occur in higher molecular mass fractions (> 10 kDa) of isolated HS.
Institut für Spektrochemie und Angewandte Spektroskopie (ISAS), Postfach 10 13 52, 44 013 Dortmund, Germany.
S
S
Haiber
H
H
Herzog
P
P
Burba
B
B
Gosciniak
J
J
Lambert
D002241:Carbohydrates / Q000032:analysis*; D002244:Carbon / Q000032:analysis*; D002623:Chemistry Techniques, Analytical; D006812:Humic Substances / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation* / Q000379:methods*; D010599:Pharmacokinetics
D002241:Carbohydrates; D006812:Humic Substances; D002244:Carbon