Resveratrol inhibits the formation of phosphatidic acid and diglyceride in chemotactic peptide- or phorbol ester-stimulated human neutrophils.
Resveratrol (trans-3,5,4'-trihydroxystilbene, Res) is a naturally occurring antioxidant found in grape berry skins and red wine. It has anti-inflammatory effects. In this study, we examined the effect of Res on the formation of phosphatidic acid (PA) and diglyceride (DG), in human neutrophils stimulated by formyl-methionyl-leucyl-phenylalanine (fMLP) or by phorbol 12-myristate 13-acetate (PMA). We measured the masses of PA and DG by using a nonradioactive method. Our results showed that Res inhibited the formation of PA in a concentration dependent manner with an IC(50) value of 42.4 and 60.9 microM in fMLP- and PMA-stimulated cells, respectively. Res also suppressed the formation of phosphatidylethanol (PEt), thereby implying inhibition of phospholipase D (PLD) activity. In addition, Res inhibited the formation of both diacylglycerol (DAG) and ether-linked acylglycerol (EAG) induced by fMLP and by PMA. Our results suggest that Res inhibition of PLD activity may contribute to its anti-inflammatory effects.
Department of Biochemistry, Tulane University Health Sciences Center, SL 43, 1430 Tulane Avenue, 70112, New Orleans, LA, USA. jtou@tulane.edu
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D002794:Choline / Q000378:metabolism; D002855:Chromatography, Thin Layer; D004075:Diglycerides / Q000378:metabolism*; D004347:Drug Interactions; D000431:Ethanol / Q000378:metabolism; D019856:Ethanolamine / Q000378:metabolism; D005994:Glycerophosphates / Q000378:metabolism; D020404:Glycerophospholipids / Q000037:antagonists & inhibitors*; D006801:Humans; D066298:In Vitro Techniques; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D010712:Phosphatidic Acids / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000031:analogs & derivatives / Q000494:pharmacology*; D013997:Time Factors
D004075:Diglycerides; D005994:Glycerophosphates; D020404:Glycerophospholipids; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D013267:Stilbenes; C051521:phosphatidylethanol; D000431:Ethanol; C020922:4-O-methyl-12-O-tetradecanoylphorbol 13-acetate; D009240:N-Formylmethionine Leucyl-Phenylalanine; D019856:Ethanolamine; D002794:Choline; D013755:Tetradecanoylphorbol Acetate; D000077185:Resveratrol