Cell-cycle research with synchronous cultures: an evaluation.
The baby-machine system, which produces new-born Escherichia coli cells from cultures immobilized on a membrane, was developed many years ago in an attempt to attain optimal synchrony with minimal disturbance of steady-state growth. In the present article, we put forward a model to describe the behaviour of cells produced by this method, and provide quantitative evaluation of the parameters involved, at each of four different growth rates. Considering the high level of selection achievable with this technique and the natural dispersion in interdivision times, we believe that the output of the baby machine is probably close to optimal in terms of both quality and persistence of synchrony. We show that considerable information on events in the cell cycle can be obtained from populations with age distributions very much broader than those achieved with the baby machine and differing only modestly from steady state. The data presented here, together with the long and fruitful history of findings employing the baby-machine technique, suggest that minimisation of stress on cells is the single most important factor for successful cell-cycle analysis.
Department of Biological Sciences, Florida Institute of Technology, Melbourne 32901, USA.
C E
CE
Helmstetter
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M
Thornton
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NB
Grover
D000596:Amino Acids / Q000378:metabolism; D002453:Cell Cycle; D002455:Cell Division; D018914:Cells, Immobilized; D003470:Culture Media / Q000737:chemistry; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D005947:Glucose / Q000378:metabolism; D008954:Models, Biological
D000596:Amino Acids; D003470:Culture Media; D005947:Glucose