Molecular cloning of rat USF2 cDNA and characterization of splicing variants.
The complete nucleotide sequence of rat USF2 cDNA was determined. In addition to the full length clone (USF2FL), four isoforms (delta1, delta2, delta3, and delta4) suggested to be generated by alternative splicing were isolated. USF2delta1 and delta2 lacked 27 and 67 internal amino acid residues, respectively. USF2delta3 and delta4 lacked most of the entire sequence but encoded short peptides of an N-terminal portion of USF2FL. Overexpression of USF2FL increased the transcription of the human high affinity IgE receptor (FcepsilonRI) alpha chain gene through specific binding to the CAGCTG motif in the first intron. On the other hand, overexpression of USF2delta1 or delta2 reduced the transcription of the human FcepsilonRI alpha chain gene. Both USF2FL and USF2delta1 bound to CACGTG as well as CAGCTG, while USF2delta2 bound to CACGTG but not to CAGCTG. These results suggested the presence of a different and definitive role of each variant in the expression of the alpha chain gene.
Foods and Pharmaceuticals Research and Development Laboratory, Asahi Breweries Ltd., Ibaraki, Japan. kyoko.takahashi@asahibeer.co.jp
K
K
Takahashi
C
C
Nishiyama
K
K
Okumura
C
C
Ra
Y
Y
Ohtake
T
T
Yokota
D017398:Alternative Splicing / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D014644:Genetic Variation; D018257:Helix-Loop-Helix Motifs / Q000235:genetics; D006801:Humans; D016350:Leucine Zippers / Q000235:genetics; D008969:Molecular Sequence Data; D011485:Protein Binding; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics; D051381:Rats; D017455:Receptors, IgE / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation / Q000235:genetics; D051720:Upstream Stimulatory Factors
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D020033:Protein Isoforms; D017455:Receptors, IgE; D014157:Transcription Factors; C497184:USF2 protein, human; D051720:Upstream Stimulatory Factors; C497187:Usf2 protein, rat