Relatedness between the coagulase gene 3'-end region and coagulase serotypes among Staphylococcus aureus strains.
The 3'-end region of the coagulase gene from 22 strains of Staphylococcus aureus including 10 standard serotype strains was sequenced, and five subgroups with 4-8 tandem repeating units were distinguished among the tested strains. Phylogenetic analysis of the 3'-end region of the coagulase gene indicated that strains belonging to the same serotype were clustered in the same branch. A phylogenetic tree of the deduced amino acid sequences revealed that the C-terminal region might not be responsible for the epitope of the coagulase protein.
Department of Microbiology, St. Marianna University School of Medicine, Kawasaki, Kanagawa, Japan. k2kane@marianna-u.ac.jp
K
K
Kanemitsu
H
H
Yamamoto
H
H
Takemura
M
M
Kaku
J
J
Shimada
D001431:Bacteriological Techniques; D003030:Coagulase / Q000096:biosynthesis* / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis; D005798:Genes, Bacterial; D010802:Phylogeny; D017421:Sequence Analysis; D017422:Sequence Analysis, DNA; D012703:Serotyping; D013211:Staphylococcus aureus / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D020080:Tandem Repeat Sequences
D003030:Coagulase; D004269:DNA, Bacterial