Three new phenolic compounds from a manipulated plant cell culture, Mirabilis jalapa.
Bioassay-guided fractionation of an organic extract of the cell mass from a manipulated plant cell culture of Mirabilis jalapa led to the isolation and subsequent identification of three new phenolic compounds, 1, 2, and 3. The isoflavone 1 and dehydrorotenoid 2 were identified as the principal antifungal principles from this plant cell culture with IC(50)'s of 25 and 48 microg/mL, respectively, against the test organism, Candida albicans DSY1024. The rotenoid 3 was inactive at 200 microg/mL in this assay.
Phytera, Inc., 377 Plantation Street, Worcester, MA 01605, USA. syang@phytera.com
S W
SW
Yang
R
R
Ubillas
J
J
McAlpine
A
A
Stafford
D M
DM
Ecker
M K
MK
Talbot
B
B
Rogers
D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification; D002176:Candida albicans / Q000187:drug effects; D002478:Cells, Cultured; D009682:Magnetic Resonance Spectroscopy; D019684:Magnoliopsida / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification*
D000935:Antifungal Agents; D010636:Phenols