Alteration of GLUR2 expression in the rat brain following absence seizures induced by gamma-hydroxybutyric acid.
We explored the involvement of the glutamate receptor subunit B (GluR2) in the mechanism of absence seizures induced by gamma-hydroxybutyric acid (GHB). The expression and distribution of GluR2 protein in rat brain were examined during and after GHB-induced absence seizures. The data indicate that GluR2 protein expression significantly decreases following the onset of absence seizures. The suppression of GluR2 expression was prolonged and it outlasted the duration of the continuous absence seizure activity. The alteration of GluR2 protein levels was accompanied by a re-distribution of GluR2 expression from laminae V to IV in cerebral cortex. We also analyzed the duration and latency of absence seizures induced by GHB 72 h following an initial GHB-induced absence seizure, a time when suppression of GluR2 protein was maximal. The second absence seizure was significantly more prolonged than the first. These data may indicate that the putative down-regulation of GluR2 following GHB-induced absence seizure could have contributed to the potentiation of subsequent seizures in animals. A related hypothesis posed by the data is that down-regulation of GluR2 is involved in the mechanisms of the maintenance of recurrent absence seizure activity once it is initiated and therefore, may contribute to the chronicity of seizures in absence epilepsy.
Department of Pediatrics, Faculty of Medicine, University of Toronto, Toronto, Ont., Canada.
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Hu
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Cortez
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Man
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Wang
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Snead
D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism* / Q000503:physiopathology; D004569:Electroencephalography; D004832:Epilepsy, Absence / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology; D006885:Hydroxybutyrates; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D018091:Receptors, AMPA / Q000378:metabolism*; D013997:Time Factors
D006885:Hydroxybutyrates; D018091:Receptors, AMPA; C111420:4-hydroxybutyric acid; C104722:glutamate receptor ionotropic, AMPA 2