Isolation and expression analysis of gibberellin 20-oxidase homologous gene in apple.
To characterize the gibberellin (GA) 20-oxidase gene in apple, the genomic and cDNA clone from "Fuji" apple (accession no. AB037114) was isolated. The deduced amino acid sequence of this cDNA showed 71% and 66% identity to those of GA 20-oxidase cloned from French bean and Arabidopsis, respectively. The transcript of this gene was detected mainly in immature seeds between 1-3 months after full bloom. These results suggested that this apple GA 20-oxidase gene might be involved in GA biosynthesis in developing apple seed.
Department of Pomology, National Institute of Fruit Tree Science, 2-1 Fujimoto, Tsukuba, Ibaraki 305-8605, Japan. kusa@affrc.go.jp
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Kusaba
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Honda
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Kano-Murakami
D000595:Amino Acid Sequence; D001483:Base Sequence; D015870:Gene Expression; D017343:Genes, Plant; D005875:Gibberellins / Q000096:biosynthesis; D006899:Mixed Function Oxygenases / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D010940:Plant Proteins; D012333:RNA, Messenger / Q000378:metabolism; D019685:Rosales / Q000201:enzymology* / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D005875:Gibberellins; D010940:Plant Proteins; D012333:RNA, Messenger; D006899:Mixed Function Oxygenases; C089210:gibberellin, 2-oxoglutarate-oxygen oxidoreductase (20-hydroxylating, oxidizing)