Epigenetic regulation of carnitine metabolising enzymes in Proteus sp. under aerobic conditions.
Proteus sp. is able to catalyse the reversible transformation of crotonobetaine into L(-)-carnitine during aerobic growth. Contrary to other Enterobacteriaceae no reduction of crotonobetaine into gamma-butyrobetaine could be detected in the culture supernatants. Activities of L(-)-carnitine dehydratase, carnitine racemasing system and crotonobetaine reductase could be determined enzymatically in cell-free extracts of Proteus sp. Small amounts of gamma-butyrobetaine were found in cell-free extracts, indicating that it accumulates in the cell and inhibits the crotonobetaine reductase. Crotonobetaine and L(-)-carnitine were able to induce enzymes of carnitine metabolism. gamma-Butyrobetaine and glucose repress carnitine metabolism in Proteus sp. Other betaines are neither inducers nor repressors. Monoclonal antibodies against purified CaiA from Escherichia coli O44K74 recognise an analogous protein in cell-free extract of Proteus sp. No cross-reactivity could be detected with monoclonal antibodies against purified CaiB and CaiD from E. coli O44K74.
Engemann
C
C
Institut für Biochemie, Fakultät für Biowissenschaften, Pharmazie und Psychologie, Universität Leipzig, Talstr. 33, D-04103, Leipzig, Germany
Kleber
H P
HP
D000217:Acyltransferases; D000332:Aerobiosis; D001622:Betaine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D002331:Carnitine / Q000378:metabolism* / Q000494:pharmacology; D004790:Enzyme Induction; D004794:Enzyme Repression; D029968:Escherichia coli Proteins; D006836:Hydro-Lyases / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism*; D011511:Proteus / Q000201:enzymology* / Q000235:genetics; D019998:Racemases and Epimerases / Q000378:metabolism*
D029968:Escherichia coli Proteins; D009097:Multienzyme Complexes; D001622:Betaine; C002889:gamma-butyrobetaine; C025237:crotonic acid betaine; D010088:Oxidoreductases; C067153:crotonobetaine reductase; D000217:Acyltransferases; C060278:crotonobetainyl-CoA carnitine coenzyme A transferase; D006836:Hydro-Lyases; D019998:Racemases and Epimerases; C091404:caiD protein, E coli; D002331:Carnitine