Structure and expression of a CTP: phosphocholine cytidylyltransferase gene from Arabidopsis thaliana.
A genomic clone, which includes the CTP: phosphocholine cytidylyltransferase (CCT) open reading frame and its 5'- and 3'- flanking non-coding regions, has been isolated from Arabidopsis thaliana and sequenced. The CCT gene is approximately 3.0 kb in length and contains 8 exons interrupted by 7 introns, which range from 74 to 626 nucleotides. All nucleotide sequences for the intron 3' splice sites are consistent with the consensus AG sequence of plant pre-mRNA processing, while the major GT consensus sequence for the 5' splice site is conserved in 5 of 7 introns. Introns 5 and 6 have a minor GC consensus sequence instead. In the 5'-flanking region there are two sequences related to a cold-responsive element found in the cold-inducible promoter of the A. thaliana cor15a gene, plus one gibberellin-response element. The results from reverse transcriptase-PCR indicate that the expression of A. thaliana CCT is regulated by temperature. The expression level of CCT increased after a 30-min treatment at 5 degrees C. When the plants were returned to 22 degrees C, the expression of CCT also decreased to the original level.
Choi
Y H
YH
Department of Biology and Institute of Molecular Cell Biology, Inha University, Inchon, Korea.
Lee
J K
JK
Cho
S H
SH
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics*; D001483:Base Sequence; D019916:Choline-Phosphate Cytidylyltransferase / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D003080:Cold Temperature; D016384:Consensus Sequence; D005091:Exons; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D007438:Introns; D008969:Molecular Sequence Data; D010713:Phosphatidylcholines / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D012322:RNA Precursors / Q000235:genetics; D022821:RNA Splice Sites / Q000235:genetics
D010713:Phosphatidylcholines; D010940:Plant Proteins; D012322:RNA Precursors; D022821:RNA Splice Sites; D019916:Choline-Phosphate Cytidylyltransferase