Effect of perfusate albumin on organ viability and vascular responses in the in vitro dual-perfused rat liver.
Inclusion of albumin in the perfusate has been previously shown to be detrimental to liver function, but its effect on hepatic vascular reactivity remains unknown. The aim of this study was to determine the effects of albumin on hepatic arterial vascular reactivity and liver viability in the isolated dual-perfused rat liver. A total of 12 rat livers were perfused with Krebs-Bülbring buffer without (Group 1) and with (Group 2) addition of 1% bovine serum albumin (BSA) through the hepatic artery and portal vein for up to 5 h. Hepatic arterial responses to acetylcholine and sodium nitroprusside were studied at 30-min intervals. Liver viability was assessed by bile volume production, release of aspartate serine aminotransferase (AST) and lactic acid dehydrogenase (LDH), and histological examination. Hepatic arterial responses to acetylcholine were significantly attenuated in Group 2. No significant differences in sodium nitroprusside responses were noted. However, bile volume production in Group 2 was significantly decreased compared to Group 1. Effluent AST and LDH release increased significantly in Group 1 but not in Group 2. Histological results showed that sinusoidal endothelial cells and hepatocytes were well preserved without significant deterioration in either group, although there was a marked decrease in vasodilatation to acetylcholine in Group 2. This data suggested that the presence of albumin in the perfusate did not improve retention of smooth muscle reactivity and reduced endothelium-dependent vasodilatation and bile volume production during perfusion. However, improved liver parenchymal cell function was observed.
Liver Sciences Unit, Academic Department of Surgery, St. Thomas Hospital, Lambeth Palace Road, SE1 7EH, London, UK.
W
W
Yang
I S
IS
Benjamin
R
R
Sherwood
J
J
Salisbury
B
B
Alexander
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001646:Bile / Q000502:physiology; D006499:Hepatic Artery / Q000187:drug effects / Q000502:physiology; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000473:pathology / Q000502:physiology*; D008102:Liver Circulation / Q000187:drug effects*; D008111:Liver Function Tests; D008297:Male; D009599:Nitroprusside / Q000494:pharmacology; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D014665:Vasodilator Agents / Q000494:pharmacology
D014665:Vasodilator Agents; D009599:Nitroprusside; D012710:Serum Albumin, Bovine; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000109:Acetylcholine