Decreased CRH mRNA expression in the fetal guinea pig hypothalamus following maternal nutrient restriction.
The regulation of corticotropin-releasing hormone (CRH) mRNA expression following maternal nutrient restriction was examined in the fetal hypothalamus. Pregnant guinea pigs were food restricted for 48 h or fed normally during late gestation. After nutrient restriction, CRH mRNA levels in the hypothalamic paraventricular nucleus of the fetus were determined using in situ hybridization and were found to be significantly decreased (P<0.0001) compared to controls. In conclusion, we have successfully sequenced the coding sequence of the guinea pig CRH gene, and have shown that a short period (48 h) of maternal nutrient restriction inhibits CRH mRNA expression in the fetal hypothalamus.
Department of Physiology, Faculty of Medicine, University of Toronto, Medical Sciences Building, 1 King's College Circle, Ont., M5S 1A8, Toronto, Canada.
K S
KS
Go
R
R
Lingas
M B
MB
Wheeler
D M
DM
Irwin
S G
SG
Matthews
D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry / Q000235:genetics; D003346:Corticotropin-Releasing Hormone / Q000235:genetics*; D005260:Female; D005333:Fetus / Q000502:physiology; D005508:Food Deprivation / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D006168:Guinea Pigs; D007031:Hypothalamus / Q000196:embryology* / Q000502:physiology; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis
D012333:RNA, Messenger; D003346:Corticotropin-Releasing Hormone