Random amplified polymorphic DNA assay as a rapid tool in screening for Neisseria meningitidis serogroup C isolates of electrophoretic type 24.
Neisseria meningitidis serogroup C (NMSC) isolates of electrophoretic type 24 (ET-24), as identified by multilocus enzyme electrophoresis, are the main cause of serogroup C meningococcal disease outbreaks and sporadic meningococcal disease in the United States. We evaluated a random amplified polymorphic DNA assay as a rapid tool to screen for isolates of ET-24 by testing 199 NMSC isolates of 51 different ETs. A sensitivity of 88% and a specificity of 87% was achieved in identification of ET-24 isolates when the patterns obtained by two primers, P1 and P5, were analyzed together.
Meningitis and Special Pathogens Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA. zma6@cdc.gov
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S
Schmink
M W
MW
Reeves
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B
Plikaytis
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T
Popovic
D004269:DNA, Bacterial / Q000032:analysis*; D004586:Electrophoresis / Q000379:methods; D006801:Humans; D008403:Mass Screening; D008589:Meningococcal Infections / Q000453:epidemiology* / Q000382:microbiology; D009345:Neisseria meningitidis / Q000145:classification* / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D012680:Sensitivity and Specificity; D012703:Serotyping; D013997:Time Factors
D004269:DNA, Bacterial