Propagation of rat parvovirus in thymic lymphoma cell line C58(NT)d and subsequent appearance of a resistant cell clone after lytic infection.
Rat parvovirus (RPV) is nonpathogenic in rats but causes persistent lymphocytotropic infection. We found that RPV was propagated in rat thymic lymphoma cell line C58(NT)D and induced apoptosis. Interestingly, a resistant subclone, C58(NT)D/R, from surviving cells after lytic infection had differentiated phenotypic modifications, such as increased cell adherence, resistance to apoptosis, and suppressed tumorigenicity.
Institute of Basic Medical Sciences and Laboratory Animal Research Center, University of Tsukuba, Tsukuba, Ibaraki 305-8575, Japan.
Y
Y
Ueno
T
T
Harada
H
H
Iseki
T
T
Ohshima
F
F
Sugiyama
K
K
Yagami
D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D002448:Cell Adhesion; D002454:Cell Differentiation; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002999:Clone Cells / Q000473:pathology / Q000528:radiation effects / Q000637:transplantation / Q000821:virology; D008223:Lymphoma / Q000473:pathology / Q000821:virology*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D017992:Parvovirus / Q000472:pathogenicity / Q000502:physiology*; D010641:Phenotype; D051381:Rats; D012692:Serial Passage; D013950:Thymus Gland / Q000473:pathology / Q000528:radiation effects / Q000637:transplantation / Q000821:virology*; D013997:Time Factors; D014407:Tumor Cells, Cultured; D014779:Virus Replication