Gas chromatography/combustion/isotope-ratio-monitoring mass spectrometric analysis of methylboronic derivatives of monosaccharides: a new method for determining natural 13C abundances of carbohydrates.
Monosaccharides were derivatized using methylboronic acid and N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA), and the delta13C values of these derivatives measured by gas chromatography/combustion/isotope-ratio-monitoring mass spectrometry to determine the original 13C-content of the monosaccharides. Comparison with the measured off-line delta13 values of the monosaccharides shows that no fractionation in 13C takes place during derivatization. The methylboronic derivatization method has proven to be a new method for natural abundance isotopic analysis of intact monosaccharides (arabinose, xylose, fucose, fructose and glucose). The method is rapid, does not involve isotopic fractionation during derivatization, and gives more precise delta13C values than other methods reported. The method was successfully applied to determine the delta13C value of glucose of the freshwater alga Scenedesmus communis.
Department of Marine Biogeochemistry and Toxicology, Netherlands Institute for Sea Research (NIOZ), PO Box 59, 1790 AB Den Burg, Texel, The Netherlands. dongen@nioz.nl
B E
BE
van Dongen
S
S
Schouten
J S
JS
Damsté
D001089:Arabinose / Q000032:analysis; D001896:Boron Compounds / Q000737:chemistry; D002247:Carbon Isotopes / Q000032:analysis*; D000460:Chlorophyta / Q000737:chemistry*; D005632:Fructose / Q000032:analysis; D005643:Fucose / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D005947:Glucose / Q000032:analysis; D015394:Molecular Structure; D009005:Monosaccharides / Q000032:analysis* / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry; D014297:Trimethylsilyl Compounds / Q000737:chemistry; D014994:Xylose / Q000032:analysis
D001896:Boron Compounds; D002247:Carbon Isotopes; D009005:Monosaccharides; D011134:Polysaccharides; D014297:Trimethylsilyl Compounds; D005643:Fucose; D005632:Fructose; C047270:N,N-bis(trimethylsilyl)-2,2,2-trifluoroacetamide; C116999:methaneboronic acid; D014994:Xylose; D001089:Arabinose; D005947:Glucose