Genomic diversity of Bartonella henselae isolates from domestic cats from Japan, the USA and France by pulsed-field gel electrophoresis.
The genomic DNA diversity of 27 Bartonella henselae and three B. clarridgeiae isolates from 18 domestic cats from Japan, the USA and France was investigated by pulsed-field gel electrophoresis (PFGE) with NotI, AscI and SmaI restriction enzymes. A great diversity of genomic patterns was found for all B. henselae, but none for B. clarridgeiae isolates. The DNA size of B. henselae and B. clarridgeiae isolates were 1.7-2.9 and 1.7Mbp, respectively. All 13 Japanese cat isolates were identified as B. henselae type I. Furthermore, three of the four Japanese cats harbored genetically different B. henselae type I isolates, suggesting for the first time co-infection with various type I isolates. One French cat and one American cat were co-infected with B. henselae and B. clarridgeiae. B. henselae type I and type II were mainly grouped in two different clusters by PFGE using SmaI endonuclease in the dendrogram.
Laboratory of Veterinary Public Health, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, 252-8510, Kanagawa, Japan. maruyama@brs.nihon-u.ac.jp
S
S
Maruyama
R W
RW
Kasten
H J
HJ
Boulouis
N A
NA
Gurfield
Y
Y
Katsube
B B
BB
Chomel
D000818:Animals; D018416:Bartonella henselae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D002371:Cat Diseases / Q000382:microbiology*; D002372:Cat-Scratch Disease / Q000382:microbiology / Q000662:veterinary*; D002415:Cats; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D005602:France; D014644:Genetic Variation / Q000235:genetics; D016680:Genome, Bacterial; D007564:Japan; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D014481:United States
D004269:DNA, Bacterial