Ocular blood flow and retinal metabolism in abyssinian cats with hereditary retinal degeneration.
OBJECTIVE
To investigate if retinal blood flow decreases with progression of the disease in Abyssinian cats with progressive retinal atrophy (PRA), to examine if the choroidal blood flow was affected by the disease, and to determine the uptake of glucose and formation of lactate in the outer retina.


METHODS
Local blood flow in different parts of the eye was determined with radioactive microspheres, in 9 normal cats and in 10 cats at different stages of PRA. Three blood flow determinations were made in each animal, during control conditions, after IV administration of indomethacin and after subsequent administration of N(omega)-nitro-L-arginine (L-NA). Blood samples from a choroidal vein and a femoral artery were collected to determine the retinal formation of lactate and uptake of glucose.


RESULTS
In Abyssinian cats with PRA (n = 10), the retinal blood flow was significantly (P < or = 0.01) lower than in normal cats (n = 9) during control conditions, 6.4 +/- 1.7 compared with 14.1 +/- 1.9 g min(-1) x (100 g)(-1). The vascular resistance in the iris and ciliary body was significantly higher in the cats at a late stage of PRA, both compared with normal cats and to cats at an early stage of the disease, whereas the choroidal vascular resistance was not significantly affected. Indomethacin had no effect on ocular blood flows in normal cats, but in cats with PRA, iridal blood flow was more than doubled after indomethacin. The retinal formation of lactate was significantly (P < or = 0.001) lower in cats with PRA than in normal cats, 0.111 +/- 0.035 (n = 8) compared with 0.318 +/- 0.024 (n = 8) micromol x min(-1). The uptake of glucose was not significantly different in cats with PRA.


CONCLUSIONS
Retinal blood flow is severely decreased in Abyssinian cats at a late stage of retinal degeneration, whereas the choroidal microcirculation is not significantly affected by the disease. At a late stage of retinal degeneration, vascular resistance in the iris is significantly increased, which at least in part could be caused by cyxlooxygenase products.
Department of Physiology, Ophthalmology Division, University of Uppsala, Sweden. sivni@imv.liu.se
S F
SF
Nilsson
O
O
Mäepea
A
A
Alm
K
K
Narfström
D000818:Animals; D001783:Blood Flow Velocity; D001786:Blood Glucose / Q000378:metabolism; D002317:Cardiovascular Agents / Q000494:pharmacology; D002415:Cats; D002829:Choroid / Q000098:blood supply*; D004195:Disease Models, Animal; D018450:Disease Progression; D004791:Enzyme Inhibitors; D005260:Female; D006339:Heart Rate; D007213:Indomethacin / Q000494:pharmacology; D019344:Lactic Acid / Q000097:blood; D008297:Male; D019335:Nitroarginine / Q000494:pharmacology; D012039:Regional Blood Flow; D012160:Retina / Q000187:drug effects / Q000378:metabolism*; D012162:Retinal Degeneration / Q000235:genetics / Q000503:physiopathology*; D012171:Retinal Vessels / Q000502:physiology*
D001786:Blood Glucose; D002317:Cardiovascular Agents; D004791:Enzyme Inhibitors; D019335:Nitroarginine; D019344:Lactic Acid; D007213:Indomethacin