Centrosome protein centrin 2/caltractin 1 is part of the xeroderma pigmentosum group C complex that initiates global genome nucleotide excision repair.
Nucleotide excision repair (NER) is carried out by xeroderma pigmentosum (XP) factors. Before the excision reaction, DNA damage is recognized by a complex originally thought to contain the XP group C responsible gene product (XPC) and the human homologue of Rad23 B (HR23B). Here, we show that centrin 2/caltractin 1 (CEN2) is also a component of the XPC repair complex. We demonstrate that nearly all XPC complexes contain CEN2, that CEN2 interacts directly with XPC, and that CEN2, in cooperation with HR23B, stabilizes XPC, which stimulates XPC NER activity in vitro. CEN2 has been shown to play an important role in centrosome duplication. Thus, those findings suggest that the XPC-CEN2 interaction may reflect coupling of cell division and NER.
Institute for Molecular and Cellular Biology and The Graduate School of Pharmaceutical Sciences, Osaka University and CREST, Japan.
M
M
Araki
C
C
Masutani
M
M
Takemura
A
A
Uchida
K
K
Sugasawa
J
J
Kondoh
Y
Y
Ohkuma
F
F
Hanaoka
D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000502:physiology*; D002460:Cell Line; D002474:Cell-Free System; D002478:Cells, Cultured; D018385:Centrosome / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone; D004249:DNA Damage; D004260:DNA Repair; D045643:DNA Repair Enzymes; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D006358:Hot Temperature; D006801:Humans; D011233:Precipitin Tests; D011485:Protein Binding; D013696:Temperature; D013997:Time Factors
D002135:Calcium-Binding Proteins; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; C056608:caltractin; C086985:RAD23A protein, human; C086984:XPC protein, human; D045643:DNA Repair Enzymes