Sulfated beta-(1-->4)-galacto-oligosaccharides and their effect on angiogenesis.
Sulfated beta-(1-->4)-galacto-oligosaccharides were prepared from an arabino-galacto-rhamno-galacturonan from Lupinus polyphyllus Lindl. by successive partial hydrolysis and SO3-pyridine sulfation in DMF. The resulting oligosaccharide polysulfates were analyzed by analytical GPC and the sulfate content was determined by ion chromatography. DP 5 and higher showed a pronounced antiangiogenic effect with scores of 0.9-1.2 for DP 7-9 using the CAM-assay. An interaction with the fibroblast growth factor FGF-2 was noticed for DP 4-12 depending on the degree of sulfation using the FGF-2-trypsin assay.
Institute of Pharmaceutical Biology, University of Regensburg, Germany.
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Käsbauer
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Paper
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Franz
D000482:Allantois / Q000098:blood supply; D020533:Angiogenesis Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002642:Chick Embryo; D002823:Chorion / Q000098:blood supply; D007887:Fabaceae / Q000737:chemistry; D008969:Molecular Sequence Data; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D010946:Plants, Medicinal; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification
D020533:Angiogenesis Inhibitors; D009844:Oligosaccharides; D011134:Polysaccharides