Stress-induced increment on total bronchoalveolar cell count in OVA-sensitized rats.
The influence of stress on total leukocyte count from bronchoalveolar lavage (BAL) was investigated in rats sensitized and challenged with ovalbumin (OVA). The animals were injected intraperitoneally with a suspension of OVA plus aluminum hydroxide in 0.9% NaCl (Day 0) and boosted at Day 7 with an identical OVA solution, administered subcutaneously. From the first to the 13th day after sensitization, rats were placed individually in a shuttle box where they received 50 escapable footshocks per day, always preceded by a sound signal (S); the responses that occurred during both S and shocks canceled the stressful stimulation. On Day 14, animals were submitted to a single session of 50 inescapable footshocks, preceded by the same S; immediately after, the animals were submitted to a 1% OVA-inhalation challenge. Results showed high levels of stress in the shocked animals as detected through both ultrasonic vocalizations (UVs) and social interaction test in an open field. Total leukocyte count in BAL from stressed animals (24 h post-OVA challenge) revealed a significant increase in the number of inflammatory cells in comparison to that measured in sensitized, nonstressed challenged rats. These data demonstrate that stress plays a relevant and important role on total bronchoalveolar cell count in OVA-sensitized rats.
Applied Pharmacology and Toxicology Laboratory, School of Veterinary Medicine, University of São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, no. 87, CEP 05508-900, Cidade Universitária, São Paulo, SP, Brazil.
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D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D002452:Cell Count; D004597:Electroshock; D006967:Hypersensitivity / Q000473:pathology* / Q000523:psychology; D007398:Interpersonal Relations; D007958:Leukocyte Count; D008297:Male; D010047:Ovalbumin / Q000276:immunology*; D051381:Rats; D017208:Rats, Wistar; D013315:Stress, Psychological / Q000473:pathology* / Q000523:psychology; D014828:Vocalization, Animal
D010047:Ovalbumin