Purification of the RelB and RelE proteins of Escherichia coli: RelE binds to RelB and to ribosomes.
The direct interaction of the Escherichia coli cytotoxin RelE with its specific antidote, RelB, was demonstrated in two ways: (i) copurification of the two proteins and (ii) a positive yeast two-hybrid assay involving the relB and relE genes. In addition, the purified RelE protein exhibited ribosome-binding activity in an in vitro assay, supporting previous observations suggesting that it is an inhibitor of translation.
Galvani
C
C
Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia V8W 3P6, Canada.
Terry
J
J
Ishiguro
E E
EE
D000992:Antitoxins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D001427:Bacterial Toxins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism* / Q000633:toxicity; D003603:Cytotoxins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism / Q000633:toxicity; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*; D051841:Transcription Factor RelB; D014157:Transcription Factors / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D000992:Antitoxins; D001427:Bacterial Toxins; D003603:Cytotoxins; D011518:Proto-Oncogene Proteins; D014157:Transcription Factors; D051841:Transcription Factor RelB