Liquid enteral diets induce bacterial translocation by increasing cecal flora without changing intestinal motility.
The aim of this study was to determine the contribution of intestinal motility and cecal bacterial overgrowth to liquid diet-induced bacterial translocation (BT). Three different commercially available liquid diets were offered to mice for 1 week. BT to the mesenteric lymph nodes (MLN), spleen, and liver were examined as well as cecal bacterial counts and populations, small bowel length and weight, and histopathologic changes in the ileal and jejunal mucosa. In addition, the effect of the various diets on intestinal motility was measured by the transit index of a charcoal mixture introduced into the stomach. The incidence of BT to the mesenteric lymph nodes was significantly and similarly increased (p < .05) in mice fed Vivonex (30%), Ensure (30%), and Osmolite (33%) compared with chow-fed controls (0%). Compared with chow-fed controls, all three liquid diets were associated with the development of cecal bacterial overgrowth (p < .01). There were no significant changes in the transit index for the three liquid diet groups compared with the chow-fed controls. BT to the MLN was induced by all three liquid diets tested, casting some doubts as to their role in preventing BT in clinical use. BT was associated with a statistically significant increase in cecal bacterial count but was not associated with gut motility changes in this model. In fact, no significant changes in intestinal motility were noted in all groups tested.
Haskel
Y
Y
Department of Surgery, Hadassah University Hospital, Mount Scopus, Jerusalem, Israel. yuvalhaskel@yahoo.com
Udassin
R
R
Freund
H R
HR
Zhang
J M
JM
Hanani
M
M
D000818:Animals; D018407:Bacterial Physiological Phenomena; D018988:Bacterial Translocation; D002432:Cecum / Q000382:microbiology*; D004750:Enteral Nutrition / Q000009:adverse effects; D005769:Gastrointestinal Motility; D005772:Gastrointestinal Transit; D008099:Liver / Q000382:microbiology; D008198:Lymph Nodes / Q000382:microbiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011897:Random Allocation; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000382:microbiology; D013997:Time Factors