Differential absorption and distribution of epidermal growth factor and insulin-like growth factor in diabetic NOD mice.
Previous studies have shown that absorption of growth factors occurs through the gastrointestinal tract and the oral cavity. The non-obese diabetic (NOD) mouse, a model for spontaneous development of type 1 insulin-dependent diabetes (IDDM), was evaluated for the absorption and systemic distribution of growth factors. Radiolabeled epidermal growth factor (EGF) and insulin-like growth factor, type I (IGF-I), were administered by gavage into the stomach or by lozenge into the sublingual vasculature of either diabetic or nondiabetic mice. After a time-dependent uptake, the levels of absorption and distribution through the tissues were measured. A similar time course of EGF absorption following gavage administration was determined for NOD and C57BL/6 mice, with a maximum tissue distribution by 30-min post infusion. Diabetic NOD mice showed similar levels of IGF uptake and tissue distribution compared with nondiabetic NOD and normal healthy C57BL/6 mice, whether administered by gavage or sublingual lozenge. On the other hand, gavage uptake and tissue distribution of EGF was significantly higher in diabetic mice when compared to sublingual administration in nondiabetic NOD or C57BL/6 healthy control mice. These findings suggest that the overall potential uptake and distribution of saliva-derived growth factors in systemic wound-healing processes is retained with diabetes onset, and may offer a new avenue to treating this complication of diabetes.
Department of Oral Biology, University of Florida, P.O. Box 100424, Gainesville, FL 32610, USA.
M Y
MY
Clarke
J
J
Brayer
K
K
Heintz
H
H
Nagashima
S
S
Cha
G E
GE
Oxford
J M
JM
Nanni
A B
AB
Peck
T
T
Zelles
M G
MG
Humphreys-Beher
D000284:Administration, Oral; D000286:Administration, Sublingual; D000818:Animals; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D007334:Insulin-Like Growth Factor I / Q000008:administration & dosage / Q000493:pharmacokinetics*; D007408:Intestinal Absorption; D007457:Iodine Radioisotopes; D051379:Mice; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D012463:Saliva / Q000378:metabolism; D013045:Species Specificity; D014018:Tissue Distribution
D007457:Iodine Radioisotopes; D004815:Epidermal Growth Factor; D007334:Insulin-Like Growth Factor I