Sinorhizobium meliloti plasmid pRm1132f replicates by a rolling-circle mechanism.
pRm1132f isolated from Sinorhizobium meliloti is a group III rolling-circle-replicating (RCR) plasmid. At least seven of eight open reading frames in the nucleotide sequence represented coding regions. The minimal replicon contained a rep gene and single- and double-stranded origins of replication. Detection of single-stranded plasmid DNA confirmed that pRm1132f replicated via an RCR mechanism.
Barran
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LR
Soils and Crops Research and Development Centre, Agriculture and Agri-food Canada, Sainte-Foy, Quebec G1V 2J3, Canada. barranlr@em.agr.ca
Ritchot
N
N
Bromfield
E S
ES
D001483:Base Sequence; D004247:DNA / Q000235:genetics; D004261:DNA Replication; D018076:DNA, Complementary; D004277:DNA, Single-Stranded / Q000235:genetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016366:Open Reading Frames / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D018741:Replication Origin / Q000235:genetics; D017422:Sequence Analysis, DNA; D016962:Sinorhizobium meliloti / Q000235:genetics* / Q000254:growth & development
D018076:DNA, Complementary; D004277:DNA, Single-Stranded; D004247:DNA