Compositional and functional changes of pulmonary surfactant in a guinea-pig model of chronic asthma.
Recent studies have found that severe surfactant dysfunction occurs during an asthma attack, but the changes in surfactant in a guinea-pig model of chronic asthma have not been studied. We therefore analysed the surfactant recovered from guinea-pigs after repeated inhalation of ovalbumin to see if the surfactant recovered from chronic asthmatic lungs would be intrinsically altered. Guinea pigs immunized through repeated inhalation of aerosolized ovalbumin (OA) were exposed to the antigen once a week for a month. Twenty-four hours after the last challenge the alveolar wash was recovered. We calculated saturated phosphatidylcholine (Sat-PC) and total protein (TP) pool sizes in alveolar spaces. Surfactant subtype conversion of large aggregate surfactant (LA) to small aggregate surfactant was studied in vitro by means of the surface area cycling technique. The phospholipid composition of LA was analysed by thin layer chromatography and the surface activity of LA was also determined. We found decreased surfactant pool sizes, decreased ratio of Sat-PC to TP in alveolar lavages in asthma groups, and surface activity of the surfactant recovered from asthmatic lungs to be inferior to that of the controls. Accelerated surfactant subtype conversion in vitro was also noted in the lungs of asthmatic animal models. In addition, the changes in phospholipid compositions which were similar to the pattern of acute lung injury suggested that alveolar inflammation might be involved in the pathogenesis of chronic asthma. These results indicate that surfactant is intrinsically abnormal in chronically asthmatic lungs.
Cheng
G
G
Department of Pulmonary Medicine and Clinical Immunology, Dokkyo University School of Medicine, Japan.
Ueda
T
T
Sugiyama
K
K
Toda
M
M
Fukuda
T
T
D000704:Analysis of Variance; D000818:Animals; D001249:Asthma / Q000209:etiology / Q000378:metabolism*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002855:Chromatography, Thin Layer; D005260:Female; D006168:Guinea Pigs; D010047:Ovalbumin / Q000009:adverse effects; D010713:Phosphatidylcholines / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D011506:Proteins / Q000032:analysis; D011663:Pulmonary Surfactants / Q000737:chemistry / Q000502:physiology*
D010713:Phosphatidylcholines; D010743:Phospholipids; D011506:Proteins; D011663:Pulmonary Surfactants; D010047:Ovalbumin