DNA-protein cross-linking in nuclei of immature and mature chicken erythrocytes.
DNA-protein cross-linkages were formed in isolated nuclei from immature and mature chicken erythrocytes by reaction with cis-diammine dichloroplatinum. On the basis of electrophoretic behaviour, the most abundant proteins involved in the cross-linking appeared to be present also in preparations of nuclear matrix. The maturation of the erythrocyte, which is accompanied by transcriptional inactivation, leads to a decrease in the amount of DNA-interacting proteins, to a loss of proteins capable of a specific recognition of DNA sequences and, unexpectedly, to the appearence of some new DNA-protein interactions. At least three cross-linked proteins were found predominantly or exclusively in nuclei of immature cells, and three others in those of mature ones. The three DNA-bound proteins, typical of mature erythrocytes, were not found among the components of a high-salt preparation of nuclear matrix. The results obtained suggest that, in addition to the well-known histone H5 and MENT protein, these newly identified DNA-bound proteins contribute to the formation of the condensed, inactive chromatin characteristic of mature erythrocyte.
Eufemi
M
M
Department of Biochemical Sciences Alessandro Rossi Fanelli and Center of Molecular Biology of CNR, University La Sapienza, Rome, Italy.
Ferraro
A
A
Altieri
F
F
Cervoni
L
L
Turano
C
C
D000818:Animals; D034961:Antigens, Nuclear; D000970:Antineoplastic Agents / Q000378:metabolism; D015153:Blotting, Western; D002467:Cell Nucleus / Q000737:chemistry; D002645:Chickens / Q000097:blood*; D002843:Chromatin / Q000737:chemistry; D002945:Cisplatin / Q000378:metabolism; D003432:Cross-Linking Reagents / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000032:analysis* / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D004912:Erythrocytes / Q000737:chemistry* / Q000648:ultrastructure; D009687:Nuclear Proteins / Q000032:analysis / Q000378:metabolism
D034961:Antigens, Nuclear; D000970:Antineoplastic Agents; D002843:Chromatin; D003432:Cross-Linking Reagents; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D004247:DNA; D002945:Cisplatin