Structure and conformation of a novel genetically engineered polysaccharide P2.
A new exocellular polysaccharide (P2) has been produced by the manipulation of a glycosyl transferase gene (aceP) involved in the biosynthesis of the polysaccharide acetan by the bacterium Acetobacter xylinum strain CKE5. The P2 polysaccharide has been studied by methylation analysis, reductive cleavage, and 1H and 13C NMR spectroscopy. The data are consistent with the structure predicted when the aceP gene is deactivated: [Molecular structure: see text]. The effect of cooling on proton NMR line width indicates a coil-helix transition in P2 at about 70 degrees C.
Colquhoun
I J
IJ
Institute of Food Research, Norwich Research Park, Colney, UK.
Jay
A J
AJ
Eagles
J
J
Morris
V J
VJ
Edwards
K J
KJ
Griffin
A M
AM
Gasson
M J
MJ
D001395:Azotobacter / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D016695:Glycosyltransferases / Q000235:genetics* / Q000378:metabolism*; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D008745:Methylation; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis / Q000737:chemistry*
D001426:Bacterial Proteins; D007202:Indicators and Reagents; D011135:Polysaccharides, Bacterial; C120750:AceP protein, Acetobacter xylinum; D016695:Glycosyltransferases