The expression of androgen receptor, cytochrome P450 aromatase and FSH receptor mRNA in the porcine ovary.
The aim of this study was to visualize the expression of androgen receptor, cytochrome P450 aromatase and FSH receptor mRNAs in various structures of porcine ovary. Porcine ovaries were frozen in liquid nitrogen, and 8 microm sections were prepared for in situ hybridization. In the small, medium and large antral follicles as well as in early, midluteal and regressing corpora lutea, mRNAs for androgen receptor, P450 aromatase and FSH receptor were detected. In small antral follicles high levels of mRNAs for androgen and FSH receptors were observed, mainly in the granulosa layer, while mRNA expression for P450 aromatase was negligible. As follicles grew, amount of mRNAs for androgen receptor and FSH receptor decreased, and that for P450 aromatase increased. Small amounts of androgen receptor mRNA were also present in corpora lutea at all examined stages. P450 aromatase mRNA was not detected in early and midluteal corpora lutea. However, regressing corpus luteum showed a weak expression of aromatase mRNA.
Słomczyńska
M
M
Laboratory of Animal Endocrinology and Tissue Culture, Institute of Zoology, Jagiellonian University, Kraków, Poland. slom@zuk.iz.uj.edu.pl
Duda
M
M
Sl zak
K
K
D000818:Animals; D001141:Aromatase / Q000235:genetics*; D003338:Corpus Luteum / Q000502:physiology*; D005260:Female; D015870:Gene Expression / Q000502:physiology; D017403:In Situ Hybridization; D006080:Ovarian Follicle / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D011944:Receptors, Androgen / Q000235:genetics*; D011962:Receptors, FSH / Q000235:genetics*; D013552:Swine
D012333:RNA, Messenger; D011944:Receptors, Androgen; D011962:Receptors, FSH; D001141:Aromatase