FIA of sildenafil citrate using UV-detection.
A flow injection analysis (FIA) of sildenafil citrate (SLD) using UV detection is described in this study. The best solvent system was found to be consisting of 0.2 M phosphate buffer at pH 8 having 10% MeOH. A flow rate of 1 ml. min(-1) was pumped and active material was detected at 292 nm. The calibration equation was linear in the range of 1x10(-6)-5x10(-6) M. Limit of detection (LOD) and limit of quantitation (LOQ) were calculated to be 3x10(-7) and 8.9x10(-7) M with a R.S.D. 1.9 and 0.6% (n=7), respectively. The proposed method was applied to the determination of SLD in VIAGRA tablet, containing 50 mg active material. The results were compared with those obtained from UV-Spectrophotometry. The results showed that there is a good agreement between FIA method and the UV-Spectrophotometry. The validation studies were realised by the related applications and the results were evaluated statistically. According to the results, insignificant difference was observed between the methods.
Department of Analytical Chemistry, Faculty of Pharmacy, Anadolu University, 26470 Eskisehir, Turkey.
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Atkosar
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Tunçel
D017022:Flow Injection Analysis; D010879:Piperazines / Q000032:analysis*; D011687:Purines; D015203:Reproducibility of Results; D000068677:Sildenafil Citrate; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D013450:Sulfones; D013607:Tablets
D010879:Piperazines; D011687:Purines; D012996:Solutions; D013450:Sulfones; D013607:Tablets; D000068677:Sildenafil Citrate