Stress-induced DNA duplex destabilization in transcriptional initiation.
Stress-induced destabilization of the DNA double helix (SIDD) is involved in several mechanisms by which transcription is regulated. This paper describes a computational method for predicting the locations and extents of destabilization as functions of DNA sequence and imposed superhelical stress. This method is used to investigate several transcriptional regulatory events. These include IHF-mediated activation of gene expression in E. coli, the bimodal control of the initiation of transcription from the human c-myc gene, and the determination of the minimal requirements for transcriptional activity in yeast. Collaborations with experimental groups have established the central role of SIDD in each of these processes.
Benham
C J
CJ
Department of Biomathematical Sciences, Box 1023, Mount Sinai School of Medicine, 1 Gustave Levy Place, NY, NY 10029, USA.
D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D004278:DNA, Superhelical / Q000737:chemistry / Q000235:genetics; D004355:Drug Stability; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D016259:Genes, myc; D006801:Humans; D008956:Models, Chemical; D009690:Nucleic Acid Conformation; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013816:Thermodynamics; D014158:Transcription, Genetic
D004278:DNA, Superhelical; D004247:DNA