Stimulation of neutrophil activation during coronary artery bypass grafting: comparison of crystalloid and blood cardioplegia.
BACKGROUND
During myocardial ischemia, activation of polymorphonuclear neutrophils (PMNs) results in the production of free oxygen radicals, which increase myocardial injury. It has been shown that PMNs also produce nitric oxide. It is not clear whether PMNs become activated as a result of their direct contact with ischemic/reperfused myocardium or if PMN activation and free oxygen radical production are effects of specific stimuli released during coronary artery bypass grafting (CABG). The aim of the current study was to evaluate plasma-mediated neutrophil stimulation and production of superoxide anion (O2) and nitric oxide in patients undergoing CABG, and to verify whether crystalloid and blood cardioplegia can modify such stimulation.


METHODS
Coronary sinus, peripheral arterial, and venous plasma samples were collected from 50 patients who underwent CABG and were divided into 2 equal groups which received either crystalloid or blood cardioplegia: directly before myocardial ischemia and aortic cross-clamping; at the beginning of reperfusion after aortic clamp release; and 30 minutes after reperfusion. O2 and nitric oxide production by PMN was evaluated by standard methods.


RESULTS
There was a significant (p < 0.05) increase in O2 production by PMN incubated with plasma obtained from the coronary sinus immediately after reperfusion in patients receiving crystalloid cardioplegia compared to blood cardioplegia. No difference was observed in plasma stimulation of nitric oxide production by PMN in the 2 groups of patients at different times during the procedure.


CONCLUSIONS
Cardioplegia may affect release of neutrophil-oriented stimuli from ischemic myocardium and modify neutrophil activation during coronary artery bypass grafting.
Department of Cardiosurgery, J Strus Hospital Poznań, Poland.
R
R
Kalawski
M
M
Baliński
P
P
Bugajski
H
H
Wysocki
R
R
Olszewski
T
T
Siminiak
D000328:Adult; D000368:Aged; D001026:Coronary Artery Bypass; D000077324:Crystalloid Solutions; D005260:Female; D006324:Heart Arrest, Induced / Q000379:methods; D006801:Humans; D007432:Intraoperative Period; D007552:Isotonic Solutions; D008297:Male; D008875:Middle Aged; D018375:Neutrophil Activation; D009569:Nitric Oxide / Q000097:blood; D010952:Plasma Substitutes; D013481:Superoxides / Q000097:blood
D000077324:Crystalloid Solutions; D007552:Isotonic Solutions; D010952:Plasma Substitutes; D013481:Superoxides; D009569:Nitric Oxide