Production of monoclonal antibodies to a tumor--associated antigen by spontaneous cell fusion.
Spontaneous cell fusion induced by the bacterium Haemophilus paragallinarum has been recently reported as an alternative technique to generate hybridomas producing monoclonal antibody (mAb). In order to investigate the advantages of this technique to produce anti-tumor monoclonal antibodies we performed comparative experiments between H. paragallinarum induced spontaneous cell fusion and polyethylene glycol (PEG) mediated fusion. Hybridomas producing monoclonal antibodies to an experimental murine lymphoma antigen, the Dalton's lymphoma associated antigen (DLAA) were generated and their sensitivity and specificity were ascertained. The spontaneous fusion yielded more number of stable and specific hybridomas than PEG mediated fusion. The results suggest the advantage of H. paragalinarum induced cell fusion for the simplified production of specific antitumor monoclonal antibodies.
Department of Biochemistry, Institute of Bioinorganic and Radiopharmaceutical Chemistry, Forschungszentrum Rossendorf, Dresden, Germany.
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Subbiah
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Ananthanarayanan
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000912:Antibodies, Neoplasm / Q000096:biosynthesis*; D000951:Antigens, Neoplasm / Q000096:biosynthesis*; D002459:Cell Fusion; D004797:Enzyme-Linked Immunosorbent Assay; D006190:Haemophilus; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D008223:Lymphoma / Q000276:immunology*; D051379:Mice; D008811:Mice, Inbred DBA; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D015395:Histocompatibility Antigens Class I; D007074:Immunoglobulin G; D007075:Immunoglobulin M; C039692:histocompatibility antigen DLA