ASCUS, mature metaplastic type. Cytologic diagnosis and follow-up.
OBJECTIVE
To study the cytologic criteria for follow-up of mature metaplastic cells within the atypical squamous cells of undetermined significance (ASCUS) category.


METHODS
Squamous epithelial abnormalities between January 1994 and June 1997 at our institution totaled 2,632 and included squamous carcinoma (1), high grade squamous intraepithelial lesions (278), low grade squamous intraepithelial lesions (875) and ASCUS (1,478). From the ASCUS group, 134 (9.06%) were metaplastic; 89 were selected for review. Criteria for case selection were follow-up with tissue biopsy or at least two Pap smears and no previous epithelial abnormality. Patients ranged from 27 to 70 years of age. Parameters tabulated included number of abnormal cells per slide, their architecture, cell size, shape, cytoplasmic hue and texture, nuclear size and contour, chromatin pattern and nucleoli. Additionally, specimens were reviewed for hormonal status and inflammation. The findings were correlated with follow-up data.


RESULTS
Cells generally appeared single or in loose, monolayered sheets of three to seven cells per group. The cells were well demarcated, polygonal or oval and ranged from 11 to 30 microns with cyanophilic or eosinophilic thickened cytoplasm. The round to oval nuclei with slight irregularity showed a minimally increased nuclear/cytoplasmic ratio with stippled chromatin. Upon review, 69 smears were confirmed as ASCUS-M. Follow-up revealed 42 with benign findings, 9 with persistent ASCUS/ASCUS-M and 18 with low grade squamous intraepithelial lesions.


CONCLUSIONS
In mature metaplastic cells with minimal atypia in patients with no previous or concurrent dysplasia, the follow-up details were similar to those described for ASCUS-superficial/immediate squamous cells. These patients could be followed conservatively.
Gupta
D
D
Department of Pathology, Magee Women's Hospital and University of Pittsburgh, Pittsburgh, Pennsylvania, USA. dgupta@mail.magee.edu
Kannan
V
V
Komaromy-Hiller
G
G
Kline
T S
TS
D000328:Adult; D000368:Aged; D002467:Cell Nucleus / Q000648:ultrastructure; D048429:Cell Size; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008679:Metaplasia / Q000473:pathology*; D008875:Middle Aged; D018307:Neoplasms, Squamous Cell / Q000473:pathology* / Q000648:ultrastructure; D065006:Papanicolaou Test; D012189:Retrospective Studies; D002583:Uterine Cervical Neoplasms / Q000473:pathology* / Q000648:ultrastructure; D014626:Vaginal Smears