IGF1 activates PKC alpha-dependent protein synthesis in adult rat cardiomyocytes.
Acute exposure to interleukin 1 beta (IL1beta) or insulin-like growth factor 1 (IGF1) promoted the translocation of PKC alpha from the cytosol to the membrane of adult rat cardiomyocytes. Western analysis demonstrated that membranal localization of PKC alpha was increased from 23% in the control to 49% after exposure to IGF1, and it was increased to 42% after exposure to IL1beta. Activation of Erk1/Erk2 by IGF1 and IL1beta was studied using a phosphorylation-specific antibody. IGF1-induced activation of p44/p42 MAP kinase was blocked by preincubation with the PKC inhibitors, bisindolylmaleimide and Gö6976, as well as the tyrosine kinase inhibitor, genistein. IGF1 increased the rate of protein synthesis, indicated by the increase in L-[(14)C(U)] phenylalanine incorporation over time, and this effect was inhibited by Gö6976.
Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, JAH 260, 1020 Locust Street, Philadelphia, Pennsylvania, 19107, USA.
A
A
Pecherskaya
M
M
Solem
D000818:Animals; D020747:Calcium Channels, T-Type / Q000187:drug effects / Q000502:physiology; D002227:Carbazoles / Q000494:pharmacology; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D019833:Genistein / Q000494:pharmacology; D015151:Immunoblotting; D007211:Indoles / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D007375:Interleukin-1 / Q000494:pharmacology*; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000378:metabolism*; D020935:MAP Kinase Signaling System / Q000187:drug effects* / Q000502:physiology; D008297:Male; D008301:Maleimides / Q000494:pharmacology; D008345:Manganese / Q000378:metabolism; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009206:Myocardium / Q000166:cytology* / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051571:Protein Kinase C-alpha; D021381:Protein Transport / Q000187:drug effects; D051381:Rats
D020747:Calcium Channels, T-Type; D002227:Carbazoles; D004791:Enzyme Inhibitors; D007211:Indoles; D007375:Interleukin-1; D007527:Isoenzymes; D008301:Maleimides; C081021:Go 6976; D008345:Manganese; D007334:Insulin-Like Growth Factor I; D019833:Genistein; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases; C088060:bisindolylmaleimide