Molecular confinement influences protein structure and enhances thermal protein stability.
The sol-gel method of encapsulating proteins in a silica matrix was investigated as a potential experimental system for testing the effects of molecular confinement on the structure and stability of proteins. We demonstrate that silica entrapment (1) is fully compatible with structure analysis by circular dichroism, (2) allows conformational studies in contact with solvents that would otherwise promote aggregation in solution, and (3) generally enhances thermal protein stability. Lysozyme, alpha-lactalbumin, and metmyoglobin retained native-like solution structures following sol-gel encapsulation, but apomyoglobin was found to be largely unfolded within the silica matrix under control buffer conditions. The secondary structure of encapsulated apomyoglobin was unaltered by changes in pH and ionic strength of KCl. Intriguingly, the addition of other neutral salts resulted in an increase in the alpha-helical content of encapsulated apomyoglobin in accordance with the Hofmeister ion series. We hypothesize that protein conformation is influenced directly by the properties of confined water in the pores of the silica. Further work is needed to differentiate the steric effects of the silica matrix from the solvent effects of confined water on protein structure and to determine the extent to which this experimental system mimics the effects of crowding and confinement on the function of macromolecules in vivo.
Eggers
D K
DK
Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095-1569, USA. eggers@chem.ucla.edu
Valentine
J S
JS
D000818:Animals; D001059:Apoproteins / Q000737:chemistry*; D002118:Calcium / Q000494:pharmacology; D002417:Cattle; D002645:Chickens; D002942:Circular Dichroism; D004229:Dithiothreitol / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D006736:Horses; D006863:Hydrogen-Ion Concentration; D007477:Ions; D007768:Lactalbumin / Q000737:chemistry*; D008786:Metmyoglobin / Q000737:chemistry*; D009113:Muramidase / Q000737:chemistry*; D009211:Myoglobin / Q000737:chemistry*; D011189:Potassium Chloride / Q000494:pharmacology; D011485:Protein Binding; D011487:Protein Conformation; D011489:Protein Denaturation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D058428:Silica Gel; D012822:Silicon Dioxide / Q000737:chemistry*; D013696:Temperature; D014466:Ultraviolet Rays
D001059:Apoproteins; D007477:Ions; D009211:Myoglobin; C010857:apomyoglobin; D008786:Metmyoglobin; D058428:Silica Gel; D011189:Potassium Chloride; D012822:Silicon Dioxide; D007768:Lactalbumin; D004492:Edetic Acid; D009113:Muramidase; D002118:Calcium; D004229:Dithiothreitol