Isolation of Encephalitozoon cuniculi using primary tissue culture techniques from a rabbit in a colony showing encephalitozoonosis.
Encephalitozoon spores were isolated in a primary tissue culture of the kidneys from an encephalitozoonosis-suspected rabbit in a municipal zoo in Hokkaido. The isolated spores were morphologically characteristic of microsporidial ones in chromotrope stain, and proven to be E. cuniculi by a polymerase chain reaction (PCR) with a species-specific primer set and by direct DNA sequencing of the PCR products.
Furuya
K
K
Hokkaido Institute of Public Health, Sapporo, Japan.
Fukui
D
D
Yamaguchi
M
M
Nakaoka
Y
Y
Bando
G
G
Kosuge
M
M
D000818:Animals; D001483:Base Sequence; D046508:Culture Techniques; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D009670:Encephalitozoon cuniculi / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D016890:Encephalitozoonosis / Q000175:diagnosis / Q000469:parasitology*; D007668:Kidney / Q000469:parasitology / Q000473:pathology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011817:Rabbits / Q000469:parasitology*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D016054:DNA, Protozoan