[Protein databank for several tissues derived from five instar of silkworm].
We attempted to construct protein databank of silkworm (Bombyx mori L.) for clarifying the gene expression and post-translational modification. Proteins from silkworm body wall, fat body and middle intestines were separated by two-dimensional electrophoresis. The N-terminal amino acid sequences of 40 proteins and their homology to proteins from other organisms were determined. 58.5% proteins were high homologous to the already reported proteins in D. melanogaster and 36.5% to those in other organisms. Only 5% of the proteins were homologous to the known protein in silkworm. The N-terminal sequences of 27 proteins were first found in silkworm, and all these data were registered in SWISS-PROT through the Internet. The results suggested that the information of gene expression and post-translational modification could be obtained by the information of protein databank.
Department of Sericulture and Apiculture Science, College of Animal Sciences, Zhejiang University, Hangzhou 310029, China.
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D000595:Amino Acid Sequence; D000818:Animals; D012831:Bombyx / Q000737:chemistry*; D016208:Databases, Factual; D015870:Gene Expression; D019476:Insect Proteins / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D008969:Molecular Sequence Data; D011499:Protein Processing, Post-Translational; D017385:Sequence Homology
D019476:Insect Proteins