Tracking Cryptosporidium parvum by sequence analysis of small double-stranded RNA.
We sequenced a 173-nucleotide fragment of the small double-stranded viruslike RNA of Cryptosporidium parvum isolates from 23 calves and 38 humans. Sequence diversity was detected at 17 sites. Isolates from the same outbreak had identical double-stranded RNA sequences, suggesting that this technique may be useful for tracking Cryptosporidium infection sources.
Division of Parasitic Diseases, Centers for Disease Control and Prevention, Mail Stop F12, Atlanta, GA 30333, USA. lax0@cdc.gov
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Xiao
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Limor
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Bern
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D000818:Animals; D001483:Base Sequence; D002417:Cattle; D016785:Cryptosporidium parvum / Q000145:classification* / Q000235:genetics; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D012330:RNA, Double-Stranded / Q000737:chemistry*; D016053:RNA, Protozoan / Q000737:chemistry*
D012330:RNA, Double-Stranded; D016053:RNA, Protozoan