Defective stem cell factor expression in c-myb null fetal liver stroma.
High levels of c-Myb are observed in immature precursor myeloid and lymphoid cells, while downregulation of c-myb accompanies terminal differentiation to a mature phenotype. This has established c-Myb as a crucial transcription factor for hematopoiesis. Further evidence for this is the embryonic death of the c-myb homozygous mutant mouse at ED15 due to defective fetal liver erythropoiesis. Cells from fetal liver of wild-type and c-myb-/- embryos were examined in detail for their hematopoietic potential and the capacity of the stroma to support wild-type hematopoiesis. The c-myb-/- fetal liver was shown to harbor sevenfold fewer spleen focus-forming cells and a similarly lower number of cells with long-term repopulating capacity (high proliferative potential cells). However, shorter term repopulating cells were not substantially reduced. c-myb-/- stromal cells were unable to support the proliferation of wild-type bone marrow lineage-negative cells. This was found to be partly due to a decrease in stem cell factor (SCF) expression while partial rescue of the stromal cell cultures was achieved through the addition of exogenous SCF. DNA binding studies for two sites within the SCF promoter demonstrated an in vitro interaction between the SCF promoter and c-Myb and transient transfection studies demonstrated that c-Myb could substantially transactivate the SCF promoter in HEK293 cells. These data explain why the c-myb-/- embryos are so impaired in their ability to establish hematopoiesis.
Peter MacCallum Cancer Institute, Melbourne, 8006, Australia.
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Sicurella
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Freeman
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Micallef
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ML
Mucenski
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Bertoncello
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Ramsay
D000818:Animals; D005786:Gene Expression Regulation; D020570:Genes, myb; D008099:Liver / Q000196:embryology / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D019089:Stem Cell Factor / Q000096:biosynthesis / Q000235:genetics*; D017154:Stromal Cells / Q000378:metabolism
D019089:Stem Cell Factor