Apoptotic activity in gestational trophoblastic disease correlates with clinical outcome: assessment by the caspase-related M30 CytoDeath antibody.
OBJECTIVE
The objective of this study was to assess apoptotic activity in gestational trophoblastic disease (GTD) and its prognostic value in hydatidiform mole (HM).


RESULTS
Expression of the specific caspase cleavage site within cytokeratin 18 was assessed immunohistochemically using the monoclonal antibody M30 CytoDeath in 12 spontaneous abortions, 22 partial and 57 complete HM, eight choriocarcinoma (CCA) and 28 normal placentas. The M30 immunoreactivity occurred predominantly in the syncytiotrophoblasts. A significantly higher M30 index in HM and CCA was found when compared with normal placentas and spontaneous abortions (P < 0.001). The M30 index of those HM which spontaneously regressed was significantly higher than those HM which developed persistent disease requiring chemotherapy (P < 0.001). The M30 index correlated with another apoptotic index previously detected by TdT-mediated dUTP nick-end labelling (TUNEL) (P = 0.007) and the proliferation index assessed by the Ki67 antigen (P = 0.034).


CONCLUSIONS
We conclude that apoptosis is important in the pathogenesis of GTD. Assessment of apoptotic activity in HM by the M30 index may be considered as an alternative prognostic indicator for predicting the clinical behaviour.
Department of Pathology, The University of Hong Kong, Queen Mary Hospital, Pokfulam Road, Hong Kong.
P M
PM
Chiu
Y S
YS
Ngan
U S
US
Khoo
A N
AN
Cheung
D000022:Abortion, Spontaneous / Q000378:metabolism; D017209:Apoptosis; D020169:Caspases / Q000378:metabolism*; D002822:Choriocarcinoma / Q000378:metabolism; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D006828:Hydatidiform Mole / Q000378:metabolism / Q000473:pathology; D020287:In Situ Nick-End Labeling; D007633:Keratins / Q000378:metabolism*; D011247:Pregnancy; D011379:Prognosis; D014328:Trophoblastic Neoplasms / Q000378:metabolism / Q000473:pathology*; D018245:Trophoblastic Tumor, Placental Site / Q000378:metabolism / Q000473:pathology; D014594:Uterine Neoplasms / Q000378:metabolism / Q000473:pathology*
D007633:Keratins; D020169:Caspases