[Effect of WT1 gene expression on cell growth and proliferation in myeloid leukemia cell lines].
OBJECTIVE
To explore the effect of WT1 antisense oligonucleotide(AS-oligo) on cell proliferation and apoptosis in myeloid leukemia cell lines.


METHODS
K562 and HL-60 cells were cultivated with WT1 AS-oligo. The cell proliferation, apoptosis, cell cycle and gene expression were examined by MTT colorimetry, FACS and RT-PCR.


RESULTS
WT1 AS-oligo could inhibit the proliferation of K562 cell and induce apoptosis of K562 and HL-60 cells. On the contrary, the growth of HL-60 cells and the expression of WT1, mdm2 and bcl-2 genes were unaffected.


CONCLUSIONS
WT1 gene is related to the proliferation and apoptosis of leukemic cells. WT1 gene could suppress cell apoptosis independent of status of p53 and bcl-2 genes. It might play an role in leukemogenesis.
Institute of Hematology and Blood Diseases Hospital, CAMS and PUMC, Tianjin 300020.
Y
Y
Mi
L
L
Wang
S
S
Bian
D017209:Apoptosis; D002455:Cell Division; D004268:DNA-Binding Proteins / Q000235:genetics*; D015870:Gene Expression; D016162:Genes, Wilms Tumor; D016158:Genes, p53; D018922:HL-60 Cells; D006801:Humans; D020014:K562 Cells; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D014157:Transcription Factors / Q000235:genetics*; D025721:WT1 Proteins
D004268:DNA-Binding Proteins; D016376:Oligonucleotides, Antisense; D014157:Transcription Factors; D025721:WT1 Proteins