Effects of serum from patients with type 1 diabetes on primary cerebellar granule cells.
Type 1 diabetes is an autoimmune disease of unknown etiology. Our previous work has shown that a factor present in serum from type 1 diabetic patients causes increased Ca2+ channel activity and apoptotic DNA fragmentation in pancreatic beta-cells. Here we examined the effects of type 1 diabetic serum on primary cerebellar granule cells (CGCs). In CGCs, exposure to type 1 diabetic serum did not cause increased apoptosis or changes in Ca2+ channel activity. However, patient serum did cause modulation of Ca2+ signals in a cell type with triangular soma that exhibited low voltage-gated Ca2+ currents. This cell was present primarily in cultures exposed to type 1 diabetic serum. The presence of low voltage-gated Ca2+ currents and long neuronal dendrites indicated that this unique cell was of neuronal origin and not of glial origin.
Chandra
J
J
Division of Toxicology, Institute for Environmental Medicine, Rolf Luft Center for Diabetes Research, Stockholm, Sweden.
Yang
S N
SN
Köhler
M
M
Zaitsev
S
S
Juntti-Berggren
L
L
Berggren
P O
PO
Zhivotovsky
B
B
Orrenius
S
S
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001323:Autoantibodies / Q000494:pharmacology*; D015220:Calcium Channels / Q000502:physiology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood* / Q000276:immunology; D006098:Granulocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D051381:Rats
D001323:Autoantibodies; D015220:Calcium Channels