Chromosomal location and evolution of a satellite DNA family in seven sturgeon species.
The Hind III satellite DNA family, isolated from the Acipenser naccarii genome, was used as a probe for fluorescent in-situ hybridization (FISH) on the karyotype of seven sturgeon species, six belonging to the genus Acipenser and one to Huso. All species except one (A. sturio) exhibit from 8 to 80 chromosome hybridization signals, mainly localized at the pericentromeric regions. Eight chromosomes with weak hybridization signals are present in H. huso and A. ruthenus, which are characterized by a karyotype with about 120 chromosomes. The species with 240-260 chromosomes, A. transmontanus, A. naccarii, A. gueldenstaedtii, and A. baerii, show from 50 to 80 signals, prevalently localized around centromeres. Moreover, A. transmontanus and A. gueldenstaedtii show from 4 to 8 chromosomes with a double signal. The phylogenetic and evolutionary relationships among sturgeon species are discussed on the basis of number and morphology of signal-bearing chromosomes and on the localization of signals.
Lanfredi
M
M
Department of Biology, University of Ferrara, Italy.
Congiu
L
L
Garrido-Ramos
M A
MA
de la Herrán
R
R
Leis
M
M
Chicca
M
M
Rossi
R
R
Tagliavini
J
J
Ruiz Rejón
C
C
Ruiz Rejón
M
M
Fontana
F
F
D000818:Animals; D002503:Centromere; D002874:Chromosome Mapping; D004276:DNA, Satellite / Q000235:genetics*; D019143:Evolution, Molecular; D005399:Fishes / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008677:Metaphase; D010802:Phylogeny; D011003:Ploidies; D013045:Species Specificity
D004276:DNA, Satellite