Genomic relatedness within five common Finnish Campylobacter jejuni pulsed-field gel electrophoresis genotypes studied by amplified fragment length polymorphism analysis, ribotyping, and serotyping.
Thirty-five Finnish Campylobacter jejuni strains with five SmaI/SacII pulsed-field gel electrophoresis (PFGE) genotypes selected among human and chicken isolates from 1997 and 1998 were used for comparison of their PFGE patterns, amplified fragment length polymorphism (AFLP) patterns, HaeIII ribotypes, and heat-stable (HS) serotypes. The discriminatory power of PFGE, AFLP, and ribotyping with HaeIII were shown to be at the same level for this selected set of strains, and these methods assigned the strains into the same groups. The PFGE and AFLP patterns within a genotype were highly similar, indicating genetic relatedness. The same HS serotypes were distributed among different genotypes, and different serotypes were identified within one genotype. HS serotype 12 was only associated with the combined genotype G1 (PFGE-AFLP-ribotype). These studies using polyphasic genotyping methods suggested that common Finnish C. jejuni genotypes form genetic lineages which colonize both humans and chickens.
Department of Food and Environmental Hygiene, Faculty of Veterinary Medicine, P.O. Box 57, University of Helsinki, FIN-00014 Helsinki, Finland. marja-liisa.hanninen@helsinki.fi
M L
ML
Hänninen
P
P
Perko-Mäkelä
H
H
Rautelin
B
B
Duim
J A
JA
Wagenaar
D000818:Animals; D015373:Bacterial Typing Techniques; D002169:Campylobacter Infections / Q000382:microbiology*; D016123:Campylobacter jejuni / Q000145:classification* / Q000235:genetics*; D002645:Chickens; D016521:Electrophoresis, Gel, Pulsed-Field; D005387:Finland; D005838:Genotype; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length; D011201:Poultry Diseases / Q000382:microbiology; D021521:Ribotyping; D012703:Serotyping