Gram-positive and Gram-negative bacteria as carrier systems for DNA vaccines.
Vaccination by intradermal or intramuscular injection of eukaryotic antigen expression vectors (so-called DNA vaccines) elicits strong cellular and humoral immune responses. A novel approach employs attenuated mutant strains of Gram-positive and Gram-negative intracellular bacteria as carriers for the delivery of DNA vaccines. This strategy allows the administration of the DNA vaccines via mucosal surfaces and a direct delivery of the plasmid DNA to professional antigen presenting cells (APC), such as macrophages and dendritic cells (DC). In this work, we have found that several Gram-negative bacteria are capable of delivering plasmid vectors to human DC. In addition, we tested the suitability of the Gram-positive bacterium Listeria monocytogenes as a vaccine carrier for the immunization of fish.
Department of Microbiology, University of Würzburg, Josef-Schneider-Str. 2, D-97074, Würzburg, Germany. gdietrich@hygiene.uni-wuerzburg.de
G
G
Dietrich
A
A
Kolb-Mäurer
S
S
Spreng
M
M
Schartl
W
W
Goebel
I
I
Gentschev
D000818:Animals; D002460:Cell Line; D003713:Dendritic Cells / Q000276:immunology; D004337:Drug Carriers; D005399:Fishes; D005822:Genetic Vectors; D006090:Gram-Negative Bacteria / Q000276:immunology*; D006094:Gram-Positive Bacteria / Q000276:immunology*; D006801:Humans; D008089:Listeria monocytogenes / Q000276:immunology; D010587:Phagocytosis; D010957:Plasmids / Q000235:genetics; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000235:genetics
D004337:Drug Carriers; D019444:Vaccines, DNA