Highly sensitive and specific time-resolved fluoroimmunoassay (TR-FIA) of cyproterone acetate and free cyproterone.
We have developed a non-isotopic TR-FIA for Cyproterone acetate and Cyproterone in plasma. Synthesis of the biotinylated tracers, biotinylated Cyproterone acetate, and Cyproterone, as well as the preparation of anti-Cyproterone acetate and anti-Cyproterone antisera are reported. The specificity of anti-Cyproterone acetate antiserum resulting from the coupling of link bridge (link bridge between steroid and BSA), on the 3-position on the steroid skeleton, allowed to carry out the Cyproterone acetate assay directly on extracted plasma (without chromatography). On the other hand Cyproterone assays require a purification step, including extraction plus chromatography, because the plasma Cyproterone acetate concentrations in Cyproterone acetate-treated women are 200 times higher than for Cyproterone. Theses plasma TR-FIA of Cyproterone acetate and Cyproterone presented the advantage of needing only small doses of radioactivity for recovery controls, and better practicability related to the only existing RIA described to date.
Fiet
J
J
Laboratoire d'Hormonologie, Hopital Saint-Louis, 1 Avenue Claude Vellefauz, 75475 Cedex 10, Paris, France.
Giton
F
F
Boudi
A
A
Boudou
P
P
Vexiau
P
P
Galons
H
H
Raynaud
J P
JP
D000152:Acne Vulgaris / Q000097:blood / Q000188:drug therapy; D000818:Animals; D000918:Antibody Specificity; D001774:Blood Chemical Analysis / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D003534:Cyproterone / Q000097:blood* / Q000276:immunology; D017373:Cyproterone Acetate / Q000097:blood* / Q000276:immunology / Q000627:therapeutic use; D005260:Female; D015200:Fluoroimmunoassay / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D006628:Hirsutism / Q000097:blood / Q000188:drug therapy; D006801:Humans; D011817:Rabbits; D012015:Reference Standards; D012680:Sensitivity and Specificity
D017373:Cyproterone Acetate; D003534:Cyproterone