The lethal interaction and formation of a lipophilic ternary complex between 2,4,5-trichlorophenol and the Cu(II)-bis(1,10-phenanthroline) complex.
When nonlethal levels of 2,4,5-trichlorophenol (0.2 mM) and the Cu(II)-bis(1,10-phenanthroline) complex [Cu(II)(OP)2] (0.1 microM) were combined, a remarkable synergistic cytotoxicity was observed as measured by the extent of bacterial inactivation. In contrast, no such synergism was observed for the combination of 2,4,5-trichlorophenol with the Cu(II)-bis(bathophenanthroline disulfonate) complex [Cu(II)(BPS)2] which has a chemical structure similar to Cu(II)(OP)2, except for the net charge. The synergism observed for 2,4,5-trichlorophenol and Cu(II)(OP)2 was found to be due to the neutralization of their opposite charge and formation of a lipophilic ternary complex which facilitated copper transport into the bacterial cells.
Zhu
B Z
BZ
Department of Cellular Biochemistry, Hebrew University-Hadassah Schools of Medicine and Dental Medicine, Jerusalem 91120, Israel. benzhan.zhu@orst.edu
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002733:Chlorophenols / Q000737:chemistry / Q000633:toxicity*; D003114:Colony-Forming Units Assay; D003300:Copper / Q000378:metabolism; D004357:Drug Synergism; D004578:Electron Spin Resonance Spectroscopy; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism; D005110:Extracellular Space / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology; D010618:Phenanthrolines / Q000737:chemistry / Q000633:toxicity*; D013056:Spectrophotometry, Ultraviolet
D002733:Chlorophenols; D016166:Free Radical Scavengers; D010618:Phenanthrolines; C118336:copper(II)(1,10-phenanthroline)3; C009534:2,4,5-trichlorophenol; D003300:Copper