Development of an immunoassay for rapid detection of ganglioside GM(1) mimicry in Campylobacter jejuni strains.
Mimicry of peripheral nerve gangliosides by Campylobacter jejuni lipopolysaccharides (LPSs) has been proposed to induce cross-reacting antiganglioside antibodies in Guillain-Barré syndrome (GBS). Because current methods for LPS characterization are labor-intensive and inhibit the screening of large numbers of strains, a rapid GM(1) epitope screening assay was developed. Biomass from two agar plates of confluent growth yielded sufficient LPS using a novel phenol-water and ether extraction procedure. Extracts of LPS were reacted with cholera toxin (GM(1) ligand), peanut agglutinin (Gal beta1-->3GalNAc ligand), and anti-GM(1) antibodies. After the assay was validated, 12 of 59 (20%) C. jejuni serostrains, including four serotypes that have not previously been associated with GBS, reacted with two or more anti-GM(1) ganglioside reagents. Subsequently, LPS extracts from 5 of 7 (71%) C. jejuni isolates and 2 of 3 (67%) C. jejuni culture collection strains bore GM(1) structures. Overall, the assay system was reliable, efficient, and reproducible and may be adapted for large-scale epidemiological studies.
Department of Microbiology, National University of Ireland, Galway, Ireland.
M M
MM
Prendergast
T U
TU
Kosunen
A P
AP
Moran
D016123:Campylobacter jejuni / Q000145:classification / Q000254:growth & development / Q000276:immunology*; D002855:Chromatography, Thin Layer; D000939:Epitopes / Q000276:immunology*; D005677:G(M1) Ganglioside / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D008070:Lipopolysaccharides / Q000737:chemistry / Q000276:immunology*; D018716:Molecular Mimicry
D000939:Epitopes; D008070:Lipopolysaccharides; D005677:G(M1) Ganglioside