Induction of class II molecules by cytomegalovirus in rat heart endothelial cells is inhibited by ganciclovir.
Cytomegalovirus (CMV) has been demonstrated to induce class II antigen expression in endothelial cells. To study whether ganciclovir (DHPG) has an effect on CMV-induced class II expression, cultured rat heart endothelial cells were infected with rat CMV (RCMV) and treated with different DHPG concentrations. Class II antigens in endothelial cells were detected by a monoclonal antibody and immunoperoxidase technique. Control cells did not express class II antigen, but during RCMV infection 92% of cells were class II-positive. DHPG treatment (1, 10, 100 and 1000 microg/ml) decreased RCMV-induced class II expression from 73% to 59%, 6% and 0%, respectively. As DHPG inhibits CMV DNA polymerase, our present results suggest that DHPG affects RCMV-induced class II expression via the inhibition of RCMV DNA replication.
Transplantation Laboratory, University of Helsinki, Finland.
J
J
Ustinov
T
T
Lahtinen
C
C
Bruggeman
P
P
Häyry
I
I
Lautenschlager
D000818:Animals; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000187:drug effects / Q000276:immunology* / Q000502:physiology; D004261:DNA Replication / Q000187:drug effects; D004730:Endothelium, Vascular / Q000166:cytology / Q000276:immunology* / Q000821:virology*; D015774:Ganciclovir / Q000494:pharmacology*; D005802:Genes, MHC Class II / Q000187:drug effects / Q000276:immunology*; D006321:Heart; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D019384:Nucleic Acid Synthesis Inhibitors; D051381:Rats; D011919:Rats, Inbred Strains
D000949:Histocompatibility Antigens Class II; D019384:Nucleic Acid Synthesis Inhibitors; D015774:Ganciclovir