Constitutive dechlorination of chlorinated ethenes by a methanol degrading methanogenic consortium.
The ability of granular methanogenic sludge to dechlorinate chloroethenes was investigated with unadapted sludge from an upflow anaerobic sludge blanket (UASB) reactor fed with methanol. The sludge degraded chlorinated ethenes, but the degradation rates were low. The addition of primary substrate was necessary to sustain dechlorination. The dechlorinating activity seemed to be constitutively present in the anaerobic bacteria. Usually, one chlorine atom was removed via reductive hydrogenolysis. Only trichloroethene (TCE) was converted to substantial amounts of vinylchloride (VC). 1,1-Dichloroethene (1,1DCE) was observed to be an important intermediate in the dechlorination by unadapted granular sludge, although previously this compound had not been commonly observed. Furthermore, the dechlorination of 1,1DCE was faster than the dechlorination of the other chloroethenes.
Department of Biomolecular Sciences, Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands. m.vaneekert@mep.tno.nl
M H
MH
van Eekert
T J
TJ
Schröder
A
A
van Rhee
A J
AJ
Stams
G
G
Schraa
J A
JA
Field
D000693:Anaerobiosis; D001421:Bacteria, Anaerobic / Q000378:metabolism*; D001711:Biotransformation; D002713:Chlorine / Q000378:metabolism; D005030:Ethylenes / Q000378:metabolism*; D019605:Euryarchaeota / Q000378:metabolism*; D006843:Hydrocarbons, Chlorinated / Q000378:metabolism*; D000432:Methanol / Q000378:metabolism; D012722:Sewage / Q000382:microbiology*; D012997:Solvents / Q000737:chemistry; D014241:Trichloroethylene / Q000737:chemistry / Q000378:metabolism
D005030:Ethylenes; D006843:Hydrocarbons, Chlorinated; D012722:Sewage; D012997:Solvents; D014241:Trichloroethylene; D002713:Chlorine; C036216:ethylene; D000432:Methanol