Androstenetriol and androstenediol. Protection against lethal radiation and restoration of immunity after radiation injury.
Androstenetriol (AET) and Androstenediol (AED) upregulate host immunity, leading to increased resistance against infections. AET augments IL-2, IL-3, IFN gamma levels, and counteracts hydrocortisone immune suppression. AET and AED at a dose of 0.75 mg/- and 8.0 mg/25-g mouse, protected 60 and 70%, respectively, of C57/BL/6J mice irradiated with a lethal dose. These hormones also protected mice irradiated with 6 Gy and infected with a coxsackievirus B4 LD50. AET significantly increased spleen lymphocyte numbers at 7, 14, and 21 days after a 6-Gy exposure. Fluorescent activated cell-sorter analysis of irradiated mice, spleen, and bone marrow showed that AET significantly augmented the myeloid precursor markers, CD11b/Mac-1, and B220 (pan B), as well as the absolute numbers of CD4+/CD8+ cells over the 21 days of testing. Overall, the data are consistent with AET/AED inducing a more rapid recovery of all hematopoietic precursors from the small number of surviving stem cells.
Loria
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RM
Department of Microbiology, Immunology and Pathology, Commonwealth University of Virginia, Medical College of Virginia, Richmond, VA 23298-0678, USA. Loria@hsc.vcu.edu
Conrad
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Huff
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Carter
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Ben-Nathan
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D045930:Anabolic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D015114:Androstenediol / Q000276:immunology / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D007109:Immunity / Q000187:drug effects*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015213:Neuroimmunomodulation; D011832:Radiation Injuries / Q000276:immunology* / Q000517:prevention & control*
D045930:Anabolic Agents; D015114:Androstenediol