pRb2/p130 gene overexpression induces astrocyte differentiation.
There are many data on the activity of the RB gene in neural differentiation and apoptosis, but the role of pRb2/p130 in neuronal and glial maturation has been far less investigated. To elucidate the role of pRb2/p130 in astrocyte development we overexpressed this protein in astrocytoma and normal astrocyte cultures by adenoviral-mediated gene transfer. In astrocytoma cells, p130/RB2 overexpression resulted in a significant reduction of cell growth and in an increased G(0)/G(1) cell population. We did not observe any induction of programmed cell death as determined by TUNEL reaction. Interestingly, pRb2/p130 overexpression induced astrocyte differentiation. Astrocyte cell cycle arrest and differentiation seemed to proceed through a way distinct from the p53 pathway.
Galderisi
U
U
Department of Experimental Medicine, Section of Pharmacology, CRISCEB, Second University of Naples, Naples, Italy. umberto.galderisi@unina2.it
Melone
M A
MA
Jori
F P
FP
Piegari
E
E
Di Bernardo
G
G
Cipollaro
M
M
Cascino
A
A
Peluso
G
G
Claudio
P P
PP
Giordano
A
A
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001253:Astrocytes / Q000737:chemistry / Q000166:cytology* / Q000502:physiology*; D001254:Astrocytoma; D018797:Cell Cycle Proteins; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D048429:Cell Size / Q000502:physiology; D050760:Cyclin-Dependent Kinase Inhibitor p27; D015870:Gene Expression / Q000502:physiology; D018014:Gene Transfer Techniques; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D020287:In Situ Nick-End Labeling; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics*; D010751:Phosphopyruvate Hydratase / Q000032:analysis; D011506:Proteins; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D016160:Retinoblastoma Protein / Q000235:genetics*; D050717:Retinoblastoma-Like Protein p130; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D025521:Tumor Suppressor Proteins; D014746:Vimentin / Q000032:analysis
C493962:Cdkn1b protein, rat; D018797:Cell Cycle Proteins; D005904:Glial Fibrillary Acidic Protein; D008869:Microtubule-Associated Proteins; D010750:Phosphoproteins; D011506:Proteins; D012333:RNA, Messenger; C493839:Rbl2 protein, rat; D016160:Retinoblastoma Protein; D050717:Retinoblastoma-Like Protein p130; D016159:Tumor Suppressor Protein p53; D025521:Tumor Suppressor Proteins; D014746:Vimentin; D050760:Cyclin-Dependent Kinase Inhibitor p27; D010751:Phosphopyruvate Hydratase