Local inhibition of nitric oxide temporarily stimulates aldosterone secretion in conscious sheep in vivo.
The effect of localized blockage of endogenous nitric oxide (NO) on basal aldosterone secretion was studied in conscious sheep with autotransplanted adrenal glands. We have shown that infusion of the NO synthase (NOS) inhibitor N(G)-nitro-L-arginine methyl ester (L-NAME; 130 microg/l blood flow) significantly stimulated basal aldosterone secretion rate (ASR). This stimulatory effect was seen up to 4 h of infusion. Beyond this time point, however, the elevated ASR level was not sustained, and it was seen to drop markedly to lower than control values at 5 h. L-NAME had no effect on cortisol secretion rate (FSR) during the first 4 h of infusion, but a significant reduction in FSR was seen by the 8-h time point. Adrenal blood flow was consistently decreased in association with long L-NAME infusion. Additionally, L-NAME was shown to have no effect on aldosterone secretion when infused systemically. We conclude that the relationship between NO and aldosterone secretion is an inhibitory one, in which NO seems to have a negative effect on basal aldosterone secretion.
Howard Florey Institute of Experimental Physiology and Medicine, University of Melbourne, Parkville, Victoria 3052, Australia. r.salemi@hfi.unimelb.edu.au
R
R
Salemi
J G
JG
McDougall
K J
KJ
Hardy
E M
EM
Wintour
D000311:Adrenal Glands / Q000098:blood supply; D000450:Aldosterone / Q000378:metabolism*; D000818:Animals; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D006854:Hydrocortisone / Q000037:antagonists & inhibitors / Q000378:metabolism; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000037:antagonists & inhibitors*; D012039:Regional Blood Flow / Q000187:drug effects; D012756:Sheep; D013997:Time Factors
D004791:Enzyme Inhibitors; D009569:Nitric Oxide; D000450:Aldosterone; D019331:NG-Nitroarginine Methyl Ester; D006854:Hydrocortisone