A redox-dependent interaction between two electron-transfer partners involved in photosynthesis.
Ferredoxin:NADP+:reductase (FNR) catalyzes one terminal step of the conversion of light energy into chemical energy during photosynthesis. FNR uses two high energy electrons photoproduced by photosystem I (PSI) and conveyed, one by one, by a ferredoxin (Fd), to reduce NADP+ to NADPH. The reducing power of NADPH is finally involved in carbon assimilation. The interaction between oxidized FNR and Fd was studied by crystallography at 2.4 A resolution leading to a three-dimensional picture of an Fd-FNR biologically relevant complex. This complex suggests that FNR and Fd specifically interact prior to each electron transfer and disassemble upon a redox-linked conformational change of the Fd.
LCCP, Institut de Biologie Structurale J.P. Ebel, CEA-CNRS, Grenoble, France.
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Morales
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Charon
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Kachalova
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Serre
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Medina
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Gómez-Moreno
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Frey
D017033:Anabaena / Q000737:chemistry / Q000201:enzymology; D002384:Catalysis; D003460:Crystallization; D018360:Crystallography, X-Ray; D004579:Electron Transport; D004583:Electrons; D005287:Ferredoxin-NADP Reductase / Q000737:chemistry* / Q000378:metabolism*; D005288:Ferredoxins / Q000737:chemistry* / Q000378:metabolism*; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism; D006860:Hydrogen Bonding; D007527:Isoenzymes / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D010788:Photosynthesis; D011487:Protein Conformation; D011522:Protons
D005288:Ferredoxins; D007527:Isoenzymes; D011522:Protons; D005182:Flavin-Adenine Dinucleotide; D009249:NADP; D005287:Ferredoxin-NADP Reductase