HPLC enantiomeric resolution of nebivolol on normal and reversed amylose based chiral phases.
Racemic nebivolol, a beta-adrenergic blocker showing very promising beta-adrenergic antagonist properties in comparison to other beta-adrenergic blockers has been resolved by HPLC under normal and reversed phase modes. The columns used were Chiralpak AD and Chiralpak AD-RH containing amylose tris (3,5-dimethyl phenyl carbamate) as the chiral selector. The mobile phases used were pure ethanol and 1-propanol. The flow rates used were 0.5, 1.0 and 1.5 ml/min. The best resolution was achieved at 0.5 ml/min. flow rate with ethanol and 1-propanol on both Chiralpak AD and Chiralpak AD-RH stationary phases. The values of infinity for both alcohols on Chiralpak AD were 1.38 while on Chiralpak AD-RH these values were 1.41 and 1.38 respectively. The values of Rs for ethanol and 1-propanol were 2.63 and 1.71 on Chiralpak AD and 1.73 and 1.76 on Chiralpak AD-RH respectively.
Aboul-Enein
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Pharmaceutical Analysis Laboratory, Biological and Medical Research Department (MBC-03), King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia. enein@kfshrc.edu.sa
Ali
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D000319:Adrenergic beta-Antagonists / Q000737:chemistry / Q000302:isolation & purification*; D000688:Amylose; D001578:Benzopyrans / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D004983:Ethanolamines / Q000737:chemistry / Q000302:isolation & purification*; D000068577:Nebivolol; D013237:Stereoisomerism
D000319:Adrenergic beta-Antagonists; D001578:Benzopyrans; D004983:Ethanolamines; D000068577:Nebivolol; D000688:Amylose