Cross reaction of recombinant equine infectious anemia virus antigen to heterologous strains and application for serological survey among horses in the field.
Cross reactivity of equine infectious anemia virus (EIAV) antigen prepared using a recombinant baculovirus containing the p26 gene of strain P337-V70 was examined by the agar gel immunodiffusion (AGID) test and enzyme-linked immunosorbent assay (ELISA). Serum samples serially collected from 13 horses experimentally infected with six different EIAV strains (two or three horses per strain) were subjected to the test. Positive reactions were observed in the AGID test and ELISA before or soon after the first feverish period and continued persistently in most of the horses. The results with recombinant antigens were essentially the same as those with the virion antigen prepared from horse cell cultures both in the AGID test and ELISA. The reactivities of the antigens were further compared using serum samples collected from horses in 1999 in certain districts of Mongolia where equine infectious anemia has been prevalent, and from horses in Japan in 1973 when EIA had not been eliminated completely from Japanese horses. These results were completely concurrent. Generally, recombinant antigens have high specificity but low cross reactivity to heterologous strains. However, the present study showed that the recombinant EIAV p26 antigen has cross reactivity to the heterologous strain and is useful for diagnosis of EIA in the field.
Sentsui
H
H
National Institute of Animal Health, Tsukuba, Ibaraki, Japan. sentsui@niah.affrc.go.jp
Inoshima
Y
Y
Murakami
K
K
Akashi
H
H
Purevtseren
B
B
Pagmajav
O
O
Sugiura
T
T
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000662:veterinary; D004859:Equine Infectious Anemia / Q000175:diagnosis* / Q000276:immunology; D006736:Horses; D005779:Immunodiffusion / Q000379:methods; D011994:Recombinant Proteins / Q000276:immunology*; D012680:Sensitivity and Specificity; D014758:Viral Core Proteins / Q000235:genetics / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D011994:Recombinant Proteins; D014758:Viral Core Proteins; C117895:equine infectious anemia virus, p26