Isolation and characterization of an N-linked oligosaccharide that is significantly increased in sera from patients with non-small cell lung cancer.
The structures of N-linked oligosaccharides present in human sera from 12 healthy volunteers and from 14 patients with non-small cell lung cancer (NSCLC) were analyzed by our recently developed partially automated systematic method. Thirty different structures of oligosaccharides were deduced, and these accounted for 84.1% of the total N-linked oligosaccharides present in human sera. All of the quantified oligosaccharide levels in healthy human sera were within twice the standard deviation. The amount of a triantennary trigalactosylated structure with one outer arm fucosylation (A3G3Fo) was found to be markedly increased in NSCLC patients in comparison to that in healthy volunteers (p < 0.01). No significant positive correlation with other clinical data was found. Serum A3G3Fo levels can thus be a novel marker for the diagnosis of NSCLC.
Laboratory of Neural Information, National Institute for Physiological Sciences, Myodaiji-cho Nishigonaka, Okazaki 444-8585, Japan.
Y
Y
Otake
I
I
Fujimoto
F
F
Tanaka
T
T
Nakagawa
T
T
Ikeda
K K
KK
Menon
S
S
Hase
H
H
Wada
K
K
Ikenaka
D000328:Adult; D000368:Aged; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood* / Q000737:chemistry* / Q000175:diagnosis / Q000503:physiopathology; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005643:Fucose / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000503:physiopathology; D008099:Liver / Q000503:physiopathology; D008175:Lung Neoplasms; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009367:Neoplasm Staging; D009844:Oligosaccharides / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification*
D009844:Oligosaccharides; D005643:Fucose