[A model for exercise-induced asthma in guinea pigs with lipopolysaccharide and metyrapone].
OBJECTIVE
To investigate the relationship between airway inflammation and exercise-induced asthma (EIA), an experimental model for EIA in guinea pigs was developed.


METHODS
27 guinea pigs was divided into 4 groups. A) LPS + MET exercise group (n = 7) had been pretreated with lipopolysaccharide (LPS 1 mg/kg, i.p.) and metyrapone (MET 50 mg/kg, i.p.) on the first day. Then lung resistance (RL) and dynamic compliance (Cdyn) were measured before and after exercise on the fourth day. B) LPS + MET non exercise group (n = 6) that pretreated with LPS and MET had nonexercise on the 4th day. C) NS exercise group (n = 7) pretreated with normal saline (NS 1 mg/kg, i.p.) on the 1st day. RL and Cdyn were measured before and after exercise on the 4th day. D) NS nonexercise group (n = 7) that pretreated with NS had no exercise on the 4th day.


RESULTS
In LPS+MET exercise group RL increased and Cdyn reduced significantly after exercise. In another 3 control groups there were no such changes either in RL or in Cdyn.


CONCLUSIONS
These observations suggest that the treatment with LPS and MET could make a model for exercise-induced asthma.
Zhongshan Hospital of Shanghai Medical University, Shanghai 200032.
Y
Y
Liang
Y
Y
Cai
L
L
Li
D000403:Airway Resistance; D000818:Animals; D001250:Asthma, Exercise-Induced / Q000139:chemically induced* / Q000503:physiopathology; D005260:Female; D006168:Guinea Pigs; D008070:Lipopolysaccharides; D008170:Lung Compliance; D008297:Male; D008797:Metyrapone
D008070:Lipopolysaccharides; D008797:Metyrapone