Functional analysis and androgen-regulated expression of mouse organic anion transporting polypeptide 1 (Oatp1) in the kidney.
Mouse Oatp1 was recently identified as a new murine member of the organic anion transporting polypeptide (Oatp) family and suggested to represent the counterpart of rat Oatp1. Northern blot analysis detected expression of several mouse Oatp-transcripts predominantly in liver and kidney. In the present study we describe the strict androgen-dependent expression of mouse Oatp1 mRNA in kidney and obtained further information about its substrate specificity using Xenopus oocytes. In addition to the previously reported estrone-3-sulfate, we demonstrate that mouse Oatp1 mediates sodium-independent uptake of the anionic steroid conjugates dehydroepiandrosterone sulfate (K(m) approximately 8 microM) and estradiol-17-glucuronide (K(m) approximately 5 microM) and also of the prostaglandin PGE(2).
Isern
J
J
Centre d'Investigacions en Bioquimica i Biologia Molecular (CIBBIM), Hospital Universitari Vall d'Hebron, Pg Vall d'Hebron 119-129, 08035 Barcelona, Spain.
Hagenbuch
B
B
Stieger
B
B
Meier
P J
PJ
Meseguer
A
A
D020413:3' Untranslated Regions; D000818:Animals; D027321:Anion Transport Proteins; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary; D019314:Dehydroepiandrosterone Sulfate / Q000493:pharmacokinetics; D015232:Dinoprostone / Q000493:pharmacokinetics; D004958:Estradiol / Q000031:analogs & derivatives / Q000493:pharmacokinetics; D005260:Female; D005786:Gene Expression Regulation; D007668:Kidney / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D013739:Testosterone / Q000031:analogs & derivatives* / Q000378:metabolism* / Q000494:pharmacology; D014982:Xenopus laevis
D020413:3' Untranslated Regions; D027321:Anion Transport Proteins; D002352:Carrier Proteins; D018076:DNA, Complementary; C025483:estradiol-17 beta-glucuronide; D013739:Testosterone; D004958:Estradiol; D019314:Dehydroepiandrosterone Sulfate; C013915:boldenone; D015232:Dinoprostone