Extremophilic orgainisms as and unexplored source fo antifungal compounds.
Extracts of the biomasses and fermentation broths of 217 extremophilic microorganisms isolated from a number of locales were screened for antifungal activity using whole-cell and mechanism-based in vitro assays. Importantly, eleven broth extracts had activity against several Candida species and Aspergillus fumigatus in whole-cell in vitro assays. One broth specifically inhibited (1,3)beta-glucan synthase activity and four specifically inhibited ketol-isomerase activity, suggesting a mode of action of the antifungal compound(s) present in these extracts. The extract from one thermophile, a novel species of Pseudomonas, was fractionated, an active compound purified and its structure determined. The compound was identified as pyochelin, a previously identified iron-binding compound with heretofore undescribed antifungal activity. To our knowledge, this is the first report demonstrating that extremophiles synthesize compounds that have antifungal activity.
Waters Corporation, Milford, MA 01757-3696, USA.
C H
CH
Phoebe
J
J
Combie
F G
FG
Albert
K
K
Van Tran
J
J
Cabrera
H J
HJ
Correira
Y
Y
Guo
J
J
Lindermuth
N
N
Rauert
W
W
Galbraith
C P
CP
Selitrennikoff
D000935:Antifungal Agents / Q000378:metabolism* / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects; D002687:Chitin Synthase / Q000037:antagonists & inhibitors; D002851:Chromatography, High Pressure Liquid; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005285:Fermentation; D005964:Glucosyltransferases / Q000037:antagonists & inhibitors; D005945:Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing) / Q000037:antagonists & inhibitors; D008565:Membrane Proteins; D008826:Microbial Sensitivity Tests; D010636:Phenols / Q000032:analysis / Q000494:pharmacology; D029702:Schizosaccharomyces pombe Proteins; D013844:Thiazoles
D000935:Antifungal Agents; D004791:Enzyme Inhibitors; D008565:Membrane Proteins; D010636:Phenols; D029702:Schizosaccharomyces pombe Proteins; D013844:Thiazoles; C025316:pyochelin; D005964:Glucosyltransferases; D002687:Chitin Synthase; C020570:1,3-beta-glucan synthase; D005945:Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing)