Characterization of hydrogen bond lengths in Watson-Crick base pairs by cross-correlated relaxation.
Hydrogen bond lengths in Watson-Crick base pairs can be characterized by cross-correlated relaxation between 1H chemical shift anisotropy and dipole-dipole coupling of 1H and its hydrogen bond acceptor 15N. As a reference, the cross-correlated relaxation between 1H chemical shift anisotropy and dipole-dipole coupling of 1H and its hydrogen bond donor 15N is used. With the two measured cross-correlated relaxation rates, an apparent hydrogen bond length can be determined, which is composed by the hydrogen bond length multiplied by a term representing the amplitude of inter-base motions. Data are presented for the 15N3-1H3...15N1 hydrogen bonds in A=T base pairs of the Antennapedia homeodomain-DNA complex with a correlation time of global rotational diffusion of 20 ns.
Institut für Molekularbiologie und Biophysik, Eidgenössische Technische Hochschule Hönggerberg, Zürich, CH-8093, Switzerland.
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D000595:Amino Acid Sequence; D016880:Anisotropy; D020029:Base Pairing; D006801:Humans; D006860:Hydrogen Bonding; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy; D009696:Nucleic Acids / Q000737:chemistry
D009696:Nucleic Acids