CD8+ T cells have an essential role in pulmonary clearance of nontypeable Haemophilus influenzae following mucosal immunization.
A rodent respiratory experimental model has proved useful for investigating the immune mechanisms responsible for clearance of bacteria from the lungs. Immunohistochemical studies in immune and nonimmune rats have identified the cellular kinetics of response to bacterial pulmonary infection for CD8+, CD4+, and gammadelta+ T cells; B cells; and the expression of major histocompatibility complex class II (MHC-II). During the course of bacterial clearance, there was no apparent proliferation or extravasation of lymphocytes, nor was there increased expression of MHC-II in nonimmune animals despite an influx of polymorphonuclear leukocytes, whereas in immunized animals there was an early influx of CD8+ and gammadelta+ T cells, followed by enhanced expression of the MHC-II marker, cellular infiltration by polymorphonuclear leukocytes, and finally an increased number of CD4+ T cells. Depletion of CD8+ T cells confirmed their vital contribution in the preprimed immune response to pulmonary infection by significantly decreasing the animals' ability to clear bacteria following challenge.
Division of Science and Design, Gadi Research Centre for Human and Biomedical Sciences, University of Canberra, Australian Capital Territory, Australia. foxwell@scides.canberra.edu.au
A R
AR
Foxwell
J M
JM
Kyd
G
G
Karupiah
A W
AW
Cripps
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology; D018414:CD8-Positive T-Lymphocytes / Q000502:physiology*; D018073:Haemophilus Vaccines / Q000276:immunology*; D006193:Haemophilus influenzae / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis; D018928:Immunity, Mucosal; D007114:Immunization; D007150:Immunohistochemistry; D008168:Lung / Q000276:immunology* / Q000382:microbiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D017208:Rats, Wistar; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000502:physiology
D018073:Haemophilus Vaccines; D000949:Histocompatibility Antigens Class II; D016692:Receptors, Antigen, T-Cell, gamma-delta