Disposition, accumulation and toxicity of iron fed as iron (II) sulfate or as sodium iron EDTA in rats.
A study was performed to provide data on the disposition, accumulation and toxicity of sodium iron EDTA in comparison with iron (II) sulfate in rats on administration via the diet for 31 and 61 days. Clinical signs, body weights, food consumption, food conversion efficiency, hematology, clinical chemistry and pathology of selected organs were used as criteria for disclosing possible harmful effects. Determination of iron and total iron binding capacity in blood plasma and non-heme iron analysis in liver, spleen and kidneys were used to assess the disposition and accumulation of iron originating from sodium iron EDTA or iron (II) sulfate. It was concluded that, under the conditions of the present study, iron is accumulated from the diet in liver, spleen and kidneys in a dose-dependent manner, and iron derived from FeEDTA is taken up and/or accumulated less efficiently in liver and spleen than iron from FeSO(4). Moreover, feeding iron up to 11.5 and 11.2 mg/kg body weight/day, derived from FeSO(4) and FeEDTA, respectively, did not result in tissue iron excess nor in any other toxicologically significant effects.
Appel
M J
MJ
Department of General Toxicology, TNO Nutrition and Food Research, PO Box 360, 3700, AJ Zeist, The Netherlands.
Kuper
C F
CF
Woutersen
R A
RA
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004396:Coloring Agents; D004032:Diet; D004435:Eating / Q000187:drug effects; D004492:Edetic Acid / Q000493:pharmacokinetics* / Q000633:toxicity*; D005290:Ferric Compounds / Q000493:pharmacokinetics* / Q000633:toxicity*; D005504:Food Analysis; D007501:Iron / Q000097:blood / Q000493:pharmacokinetics*; D008297:Male; D019159:Nonheme Iron Proteins / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014018:Tissue Distribution
D004396:Coloring Agents; D005290:Ferric Compounds; D019159:Nonheme Iron Proteins; C024823:ferric sulfate; D004492:Edetic Acid; D007501:Iron; C019179:Fe(III)-EDTA