Regulation of functional activity of bone marrow hemopoietic stem cells by erythroid cells in mice.
Transplantation of erythroid and bone marrow cells to irradiated mice stimulated exogenous colony formation. Pretreatment of erythroid cells with specific rabbit antiserum to erythroblasts abolished this effect. The reverse transcriptase polymerase chain reaction revealed the presence of mRNA for interleukin-1alpha, interleukin-1beta, interleukin-3, interleukin-6, and granulocyte-macrophage colony-stimulating factor in erythroid cells. Granulocyte-macrophage colony-stimulating factor was found in the conditioned medium from erythroid cells. Thus, erythroid cells stimulated colony-forming activity of bone marrow cells, which was probably mediated via cytokine synthesis (e.g., granulocyte-macrophage colony-stimulating factor).
Institute of Clinical Immunology, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk. ici@online.nsk.su
S V
SV
Sennikov
T V
TV
Inzhelevskaya
L V
LV
Eremina
V A
VA
Kozlov
D000818:Animals; D001483:Base Sequence; D003114:Colony-Forming Units Assay; D017077:Culture Media, Conditioned; D017931:DNA Primers / Q000235:genetics; D004900:Erythroblasts / Q000276:immunology / Q000502:physiology* / Q000637:transplantation; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics / Q000378:metabolism; D006410:Hematopoiesis / Q000235:genetics / Q000276:immunology / Q000502:physiology; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000276:immunology / Q000502:physiology*; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000235:genetics; D007377:Interleukin-3 / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits
D017077:Culture Media, Conditioned; D017931:DNA Primers; D007375:Interleukin-1; D007377:Interleukin-3; D015850:Interleukin-6; D012333:RNA, Messenger; D016178:Granulocyte-Macrophage Colony-Stimulating Factor