Expression of immediate early genes, HSP70, and COX-2 mRNAs in rat stomach following ethanol ingestion.
The aim of this study was to show the temporal and spatial molecular responses in the rat stomach that follow absolute ethanol-induced acute mucosal injury. Intense signals for immediate early genes (IEG)/transcriptional factors such as c-fos, c-jun, and nerve growth factor-induced gene-A (NGFI-A) mRNAs were observed in the superficial mucosa and in the blood vessels from 15 min to 6 hr after administration, peaking at 15-30 min. Signals for heat shock protein (HSP) 70 mRNA were also detected in the superficial mucosa, in the fibroblasts around gastric erosions, and in the blood vessels from 15 min to 6 hr (peak at 1-2 hr). The signals for cyclooxygenase-2 (COX-2) mRNA were up-regulated in the surface mucous cells that surround the erosions from 30 min to 6 hr (peak at 60-90 min). These findings suggest that IEG, HSP70, and COX-2 are involved in gastric mucosal restitution in different ways.
Department of Anatomy and Neurobiology, Wakayama Medical University, Japan.
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M
Saika
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T
Ueyama
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E
Senba
D000428:Alcohol Drinking / Q000009:adverse effects*; D000818:Animals; D051546:Cyclooxygenase 2; D005753:Gastric Mucosa / Q000737:chemistry*; D017781:Genes, Immediate-Early / Q000235:genetics*; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis / Q000235:genetics*; D007527:Isoenzymes / Q000032:analysis / Q000235:genetics*; D008297:Male; D011451:Prostaglandin-Endoperoxide Synthases / Q000032:analysis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D015854:Up-Regulation
D018840:HSP70 Heat-Shock Proteins; D007527:Isoenzymes; D012333:RNA, Messenger; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases