Effects of batroxobin on spatial learning and memory disorder of rats with temporal ischemia and the expression of HSP32 and HSP70.
The effect of Batroxobin on spatial memory disorder of left temporal ischemic rats and the expression of HSP32 and HSP70 were investigated with Morri's water maze and immunohistochemistry methods. The results showed that the mean reaction time and distance of temporal ischemic rats in searching a goal were significantly longer than those of the sham-operated rats and at the same time HSP32 and HSP70 expression of left temporal ischemic region in rats was significantly increased as compared with the sham-operated rats. However, the mean reaction time and distance of the Batroxobin-treated rats were shorter and they used normal strategies more often and earlier than those of ischemic rats. The number of HSP32 and HSP70 immune reactive cells of Batroxobin-treated rats was also less than that of the ischemic group. In conclusion, Batroxobin can improve spatial memory disorder of temporal ischemic rats; and the down-regulation of the expression of HSP32 and HSP70 is probably related to the attenuation of ischemic injury.
Neurotransmitter Research Laboratory, Department of Neurology, Chinese PLA General Hospital, Postgraduate Military Medical School, Beijing 100853.
W
W
Wu
P
P
Kuang
S
S
Jiang
X
X
Zhang
J
J
Yang
N
N
Sui
C
C
Albert
P
P
Kuang
D000818:Animals; D012103:Batroxobin / Q000627:therapeutic use*; D002545:Brain Ischemia / Q000150:complications / Q000188:drug therapy* / Q000378:metabolism; D015536:Down-Regulation; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis*; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006419:Heme Oxygenase (Decyclizing); D007859:Learning Disabilities / Q000188:drug therapy* / Q000209:etiology; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D008569:Memory Disorders / Q000188:drug therapy* / Q000209:etiology; D010105:Oxygenases; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012910:Snake Venoms / Q000201:enzymology / Q000627:therapeutic use*; D013037:Spatial Behavior / Q000187:drug effects; D013702:Temporal Lobe / Q000098:blood supply
D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D012910:Snake Venoms; D010105:Oxygenases; D006419:Heme Oxygenase (Decyclizing); C491523:Hmox1 protein, rat; D012103:Batroxobin