Central CRH suppresses specific antibody responses: effects of beta-adrenoceptor antagonism and adrenalectomy.
Central corticotropin releasing hormone (CRH)-induced activation of the sympathetic nervous system and/or the pituitary-adrenal axis is hypothesized to mediate suppression of in vivo specific antibody responses. To test whether beta-adrenergic receptor activation is involved in the immunosuppressive effects of central CRH, rats were pretreated with propranolol or saline before intracerebroventricular infusion of CRH and immunization with keyhole limpet hemocyanin (KLH). KLH (3 microg/kg) immunization induced significant increases in circulating levels of antigen-specific IgM and IgG. Central infusion of CRH (200 pmol) suppressed both IgM and IgG responses. Pretreatment with propranolol (20 mg/kg IP) reversed CRH-induced suppression of IgG responses but had no effect on IgM levels. To test whether adrenal activation also plays a role in the effects of KLH on specific antibody responses, a separate group of animals underwent adrenalectomy prior to CRH infusion and immunization with KLH. As compared to nonadrenalectomized control rats, adrenalectomized rats showed a reduction of antibody responses, and CRH failed to induce a further suppression of IgM or IgG responses in adrenalectomized animals. Collectively, these data suggest that beta-adrenoceptors mediate the suppression of primary antibody responses induced by central CRH. Moreover, the adrenals may promote optimal primary antibody responses after exposure to physiological levels of antigen.
Department of Psychiatry, University of California, San Diego, USA.
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Friedman
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Irwin
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000315:Adrenalectomy; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology*; D000818:Animals; D003346:Corticotropin-Releasing Hormone / Q000276:immunology* / Q000378:metabolism; D006433:Hemocyanins / Q000494:pharmacology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007075:Immunoglobulin M / Q000097:blood / Q000276:immunology; D008297:Male; D015213:Neuroimmunomodulation / Q000187:drug effects / Q000276:immunology; D011433:Propranolol / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D011943:Receptors, Adrenergic, beta / Q000276:immunology* / Q000378:metabolism; D013312:Stress, Physiological / Q000276:immunology / Q000378:metabolism; D013564:Sympathetic Nervous System / Q000276:immunology / Q000378:metabolism
D000276:Adjuvants, Immunologic; D000319:Adrenergic beta-Antagonists; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011943:Receptors, Adrenergic, beta; D006433:Hemocyanins; D003346:Corticotropin-Releasing Hormone; D011433:Propranolol; C032808:keyhole-limpet hemocyanin