Evaluation of PCR methods for rapid identification and differentiation of Streptococcus uberis and Streptococcus parauberis.
Streptococcus uberis and Streptococcus parauberis reference strains and isolates obtained from routine diagnostics were investigated by PCR with oligonucleotide primers designed according to species-specific parts of the 16S rRNA gene, the 23S rRNA gene, and the 16S-23S rRNA intergenic spacer region of both species. All three primer pairs allowed an identification of 67 isolates as S. uberis and 4 isolates as S. parauberis.
Institut für Tierärztliche Nahrungsmittelkunde, Bakteriologie und Hygiene der Milch, der Justus-Liebig-Universität Giessen, Giessen, Germany.
A A
AA
Hassan
I U
IU
Khan
A
A
Abdulmawjood
C
C
Lämmler
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D020459:Genes, rRNA; D008414:Mastitis, Bovine / Q000382:microbiology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013290:Streptococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013291:Streptococcus / Q000145:classification* / Q000235:genetics; D013997:Time Factors
D021903:DNA, Ribosomal Spacer; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S