Non-specific activation of the epithelial sodium channel by the CFTR chloride channel.
The genetic disease cystic fibrosis is caused by mutation of the gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR). Controversial studies reported regulation of the epithelial sodium channel (ENaC) by CFTR. We found that uptake of (22)Na(+) through ENaC is modulated by activation of CFTR in oocytes, coexpressing CFTR and ENaC, depending on extracellular chloride concentration. Furthermore we found that the effect of CFTR activation could be mimicked by other chloride channels. Voltage- and patch-clamp measurements, however, showed neither stimulation nor inhibition of ENaC-mediated conductance by activated CFTR. We conclude that the observed modulation of (22)Na(+) uptake by activated CFTR is due to the effect of CFTR-mediated chloride conductance on the membrane potential. These findings argue against the notion of a specific influence of CFTR on ENaC and emphasize the chloride channel function of CFTR.
Nagel
G
G
Max-Planck-Institute of Biophysics, Kennedyallee 70, D-60596 Frankfurt/M., Germany. Georg.Nagel@mpibp-frankfurt.mpg.de
Szellas
T
T
Riordan
J R
JR
Friedrich
T
T
Hartung
K
K
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics / Q000378:metabolism*; D004566:Electrodes; D053503:Epithelial Sodium Channels; D004848:Epithelium / Q000378:metabolism; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D017136:Ion Transport; D009865:Oocytes / Q000378:metabolism; D018408:Patch-Clamp Techniques; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D015222:Sodium Channels / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis
C505032:CFTR protein, human; D053503:Epithelial Sodium Channels; D011994:Recombinant Proteins; D015222:Sodium Channels; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D012964:Sodium