Detoxification effect of iron-encaging zeolite-processed water in tributyltin-intoxicated Euglena gracilis Z.
In our previous paper, we reported the restoration promoting effects of mineral-encaging zeolite-processed water, especially of a Fe-encaging one, on tributyltin chloride (TBTCl)-intoxicated Euglena gracilis. This present study extends the investigation on the behavior of TBTCl and a xenobiotic enzyme, cytochrome P-450, in Euglena cells incubated with or without Fe-encaging zeolite-processed water (FeZW). Subcellular fractionation of TBTCl-intoxicated Euglena cells, atomic absorption spectrophotometry, and GC analyses showed that TBTCl was rapidly incorporated into the cells to halt cell motility. GC-MS showed that FeZW promoted conversion of TBTCl to dibutyltin (DBT) as the major metabolite in the microsomal fraction of the cells. An in vitro incubation system with heat-treated microsomes did not convert TBTCl to DBT. The contribution of cytochrome P-450 in the microsomal fraction was suggested by an immunochemical method. The results suggest that the improvement of detoxification by FeZW in the TBT-intoxicated Euglena cells should be due to activation of biotransformation system of the Euglena cells by FeZW.
Department of Marine Bioresources, Faculty of Fisheries, Hokkaido University, Hakodate, Japan.
M
M
Ohta
K
K
Nakamura
T
T
Kubo
T
T
Suzuki
D000818:Animals; D001711:Biotransformation; D015153:Blotting, Western; D002458:Cell Fractionation; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005056:Euglena gracilis / Q000737:chemistry / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D007501:Iron / Q000737:chemistry*; D008861:Microsomes / Q000378:metabolism; D051381:Rats; D014220:Trialkyltin Compounds / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D014867:Water / Q000737:chemistry*; D017641:Zeolites / Q000737:chemistry*
D014220:Trialkyltin Compounds; D014867:Water; D017641:Zeolites; C011559:tributyltin; D003577:Cytochrome P-450 Enzyme System; D007501:Iron